LOCUS       DAWAOG010000001        18095 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_639_length_18095_cov_2.324612, whole genome shotgun sequence.
ACCESSION   DAWAOG010000001 DAWAOG010000000
VERSION     DAWAOG010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 18095)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18095)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18095
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_639_length_18095_cov_2.324612"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3296..3324
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4724..4733
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5460..5469
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17415..17424
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4366 a         4200 c         4150 g         5320 t
ORIGIN      
        1 tactgataga atgcggattt ttatcaaatc ccgacgatgc ggcaaatctt tccgacaggg
       61 actatcagcg caaggtcgct tatacaatat ataacggtat acttacatat cttacctcaa
      121 agcctgccga taacgcttcg ctgactaccg cacagcagac gactgttata ccgcagtcaa
      181 ccgctgaacc ggcgggcagt gttcccgaaa acagcggtga tatagcaagc gtggcacaat
      241 aagacaaacg gctttggaaa ggacgtataa atggcaaaat caaaatcagt atacatatgc
      301 agtgaatgcg ggcaggagta cccaaagtgg agtgggcgct gtaacgcctg cggagcatgg
      361 aacacgcttg aagaaacgct catacagcca aaatcacacg gacttgcaaa aggctcgggc
      421 gcaggcagat tgccgctcgc aagcataaat aatctttcgt tcaaggacga aacgcggtat
      481 gaaacaggaa tgtcagagct tgacagggtc ctcggcggag gtcttgtcaa aggctcgctg
      541 gtgctgctcg gcggcgaccc cggtatcggc aaatcgacgc ttctgttaca gatatgcggc
      601 tgtcttgcaa aggacaaaac cgtgctgtac atatcgggag aagagagtga acgacagata
      661 aagctccgcg cagacaggct cggagttgca aatgacggac tttatatcag cgcctgcacc
      721 gactgtgata ccataataga gggtgtgcgc gaaaacaagc ccgatgtagt gataatcgac
      781 tcgatacaga caatgcaatt atcggagctc cagtccgtac cgggaagtct tgttcaggta
      841 agggagtgca cctcggcatt tatgcagatg gcaaagagcc ttgaggtcgc agtgttcctt
      901 gtaggtcacg tcaacaagga cggcggcatt gcaggtccta aggtgcttga gcatatagta
      961 gacacggtgc tgtactttga gggcgacaag cagctcagct acagaatttt aagagccgca
     1021 aagaaccgtt tcggctcaac caacgaaata ggcgtgtttg agatgcagga caaaggactt
     1081 gaacaggtcg aaaacccgtc cgctatgctc ctgcttggca gacccgcagg tgtcagcggt
     1141 ataacagtac tgtgtacggt cgagggtaca cgtccgatac ttgcggaggt tcaggcgctt
     1201 gtcagcaaga cctcgttctc agccccgcgc agaatgacta cgggctttga ctacagcagg
     1261 ctgtgtgtgc ttcttgctgt acttgaaaag aggacaggct ataatttcgg cggatacgat
     1321 gtatatataa atgtgatcgg cggactgaaa atagacgagc cggcaagcga ccttgcgata
     1381 tcgcttgcac tgtattcggg actgcgcgac ataccgatag gagatgatgt cgcggtattc
     1441 ggcgaggtag gtcttggcgg agagataaga gcggtatcgc atataagaga gcgtgtgcgc
     1501 gaggcggcgc gaatgggatt tgaaaaatgt atcgtaccga aatcatcgct taaaaatctt
     1561 gacaagagcg aaaattacgg aataagcata tacggcgtgg ctaatcttca gcaggcgttc
     1621 aaggtgcttg agatgttcac aaaagaaagc gaagggaaca aagaatgaag cttaccgaca
     1681 tttcaacaat aagacagctt tttgaaaagc acggattttc atttacaaaa tcactcgggc
     1741 agaactttct gataaatccg actgtctgcc cgaaaatcgc cgaaatgggc ggagcgggtg
     1801 aaaatgtcgg cgctattgaa ataggcacag gtataggcgt gcttacaaga gagcttgcgg
     1861 agcgttgcga aaaggtcgtt gctatcgaaa tagacacggg gctgaagcct attcttgacg
     1921 aaacgcttga agaatacgac aatgttgaag tgatcttcgc ggacgttatg gaaaccgatc
     1981 ttaataaact tatagctgag aaattttccg gtatggacgt tatcgtctgc gctaatcttc
     2041 cctactacat tacttcaccc gtaataatga agcttcttga agaaaagctc ccgataaagg
     2101 cgataacggt aatggtacag cttgaggcgg cggacaggat atgcgccgcg gtaggcagtc
     2161 gtgagtgcgg agctatcacc gcatcgataa actattacgc aaagccgaaa aagcttttcc
     2221 gcgtaaaccg tggttcattt atgcccgcgc ccaatgttga cagtgcggtc ataagccttg
     2281 aacgatatga acagccgccg tacaaggtcg ataacgagcc gctttttttt gaggtaataa
     2341 agcaggcgtt tgctcagcgc agaaaacagc ttacgaaccc tgtttcggct tatttcaaga
     2401 tatcgaaagc cgcgcttgca aaaaagatga ctgaaagcgg tattaccgcg actgcaagag
     2461 cagaacagct tacgatggcg gagcttgtaa ggctgtatga gatagtgaat gagttgacaa
     2521 ctaaataaac ataaaagcca cgatgtaaaa atcgcggctt ttgctttttg ggtacttata
     2581 cgacaacaaa gcccgggtga tgatttcggt caacgcccgg gcttcgtgct attcaattaa
     2641 agcaaatcaa aagcttactt agctaacgtc tttccactca cacttccgta attaccataa
     2701 agtgccgtct tgccgctcat gtaataagcc ttgaccctga acttgtaagt cgtgttctta
     2761 gcaagtccgg ctttcctgaa cgttgtagta ttgccgtttg ttatcttggc aactcttacc
     2821 cacttaccgc ccttgtacat ttcaacaatg tagccttgcg cggaagcgtt cttagcccag
     2881 tttactctga gtgcgtcctt agccttgcca gctatcttaa cgcccttaac tattgacggg
     2941 ttagttctgg cagtaacagt agcgctgtag ttaccgtaaa gcgcggtctt accgctcatc
     3001 ttatatgctc ttactctgaa cttgtacaca gtcgaagcct taagtcctgc ttttctgaat
     3061 gtggttgtgc tgttgtttgt gatcttagcg gctcttaccc acttgccgcc cttgtacatt
     3121 tcaacaatgt atccgtcagc agatgtattc ttagtccagt tgatacggag tgcgtcagcc
     3181 gctctgccgg tgagtttagc gccctttata actgacggat ttgtccttac cgcaacagtc
     3241 gcgctgtacg cgccgtaaac cgcagtcttg ccgctcattt tatacgctct tatccnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnttgtaa gagccaccca tttctgacca ttccacttct
     3361 gtaactcata gcctgtagct gatgtattct tgttccactg aagtgttacg cttattgaag
     3421 tctttgactt tgttttaaat cctgtcaccg cagacggaag agtacatacg gttatctccg
     3481 caggtacgcc gtatataacc tccgtgccgt ccttgaaaca cagcactctg agtttatact
     3541 ctgtgctggg gtttagtcca ttcacctcag cagtggtctt gtttatacct gtattcttta
     3601 ccgctgtagt ccattttccg ttcttgtaga cctgtactgc atatccgtca gctgtattgt
     3661 tcttgctcca gctgagagtt accgacttat cggtacgctt tgtaagcttt acagataccg
     3721 gctttgtcgg cgctgttctt tcggagaata ttgtgctgta ttcactgtat acgggctttc
     3781 cgtccaccat aagatacgcc gcaaatctga actgataaac ggttgacgga gcgagcttat
     3841 ctgctgtgta acccgtaacg tcataagcaa ggtcagctat tgtttcccac ttgccgtcga
     3901 taaactgttc taccacatag ccgtccgcgt tatcggatac gttccagccg aatgtaaggc
     3961 tttcgcttgt acgtcctgtt ctctttacat tggtcagcgg atcgaccttg agcatatcca
     4021 caaagctgtc gttgtaagaa taaccgcata ccgagcattc gtgatgcgtg tagccgtccg
     4081 atgtatatgt aggcgctatt accgtatcaa caaatttgtg cttattcagc acgttggttt
     4141 cttcaagtcc acagtcggcg cagattcttg actcatagcc tatttcggtg caagtagact
     4201 cctttaccgt tttccacata ccgaatgagt gcttttcgca gagatagtca aaatcgagtt
     4261 tattcttttt ggcaaaatct tcggctatcg tgcctttata acaatatact gttgtattct
     4321 ttccgaaata acatccgttc ggaatttcgg tgagacttct tggcagtgct acggatttta
     4381 acttttcaca cctgctcaga tttgatttta ttactttcat actttcggga taagtaattt
     4441 tttcaattga atcggcccaa ctaaaagccc ccagctcttc ttctacgccg taaagcataa
     4501 ttactgtttt aagaccatag caattatcga atacacctga tgaaacagtc ttgacgctac
     4561 ccggtatgat tatttcttcc agaccgctac aggaagaaaa agcgcctttt tcaataattg
     4621 taaccgaatc cggtatgtta acatacttga gttttccggc acttgagcat gtgctttcac
     4681 atatttttgt cgttccttgt tcaaattcga cagttaccag accnnnnnnn nnnccgcttg
     4741 cgtcaaacgg agcataaccg attttcgtaa gagaagccgg taaagttatg ttatacaagc
     4801 tgtcacagct tgcaaacgcg tgatagccca gacttgtcag attttcaggc agcttaacgt
     4861 tattcagata atcgcaatta tagaaaacct gttcaccgat ggtcaatacc gtatcaggaa
     4921 tatttacctg ctgaatggtc ttgttggagt agaaagcctc atcacctata gctgtaaccc
     4981 ttgaaccttt gatttccgac gggatatcga ccgatgtttc cgagccgtta tatgccgtga
     5041 tcgtcagcgt gtcatcgtcg ttataagtgt acttataatc gccgtaggta gcggcaccgg
     5101 cacacactgc gcatattgcg gctgttgctg tgaatgtagc aatgcctaat aataactttg
     5161 ctattttcat aaaactcttc ctttcattga taaatactga tataatccta tcattatcta
     5221 tcagtttatc acattgaaag tgaattttct actgcatttt tccaaggaat attctcccga
     5281 atttttcgga tatacgcaaa aacgccccgc aaaagcgggg cgccagattg tagaaaaaat
     5341 ctgttttttc aaaaaagtgc ttgtcctcaa ttcctatccc caaacccctt tcccaagaaa
     5401 ggggctgagt gtggggctgc cgccctcacc aagcaaggac gcttgcagtc agccgcacan
     5461 nnnnnnnnng tcgcacacag tcgctgcacg atgcagcgac accaacgact gacaacccgc
     5521 attggggcta cgcccaaagc aaggacgctt tggttgcttg ccaaaagttt tgcacgatgc
     5581 aaaaccaaca tagcaagcaa cgctaaacac ccattttcaa aaattatata ggttttttcg
     5641 acatactgaa acgccccgca aaagcggggc gctattatcg ttattaagct gtcattaaga
     5701 atagtccaca acgtcaagcc atgtgaagag gagatccttc tcttccttgc ttgtaacacc
     5761 ctttgcgagt gtagaagccg ccatgatggg taaccattcc tggacatatg tctttgctgt
     5821 atttgtcttc ttgcagaaga ggttgagata cttctcggcc gtttctgtcg atgtcagagc
     5881 aagctttaag tatgtcttgg ctacatctgc actggcatta cctgcggaag cgcctatcca
     5941 gtcaactatg taaaccttgt tgtcatctgt aacaactata ttctcaggtc cgaagttgcc
     6001 gtggcagagc tttacgtgct tgggtgtgct gtcaagtctt gtgagaagat cgtatctctt
     6061 gatgtggtcg ataacgtcaa ggctctcgat ctctgctctc aggttatcct tgagctttga
     6121 aatgccggtg atcttcttcg cgtgaatgct gagctggagc tcaaccatat catcaagata
     6181 gccgtcgctg ttctcaggct cttcctgcat gatctgtgca agcgtcttac ccttgacaag
     6241 ctctgttgct attgcccatt tgccgtcgat aagttcaact tcttttatct tgggaacgtt
     6301 aagtccggtt tcttcaacct ttgaatccat gaacgcctca tagaaaacta ctgtcttggg
     6361 cgcatcgggt ttgaacacct ttactgcaag gtcgccgctc tcgtatacgt caacggtttt
     6421 accttcggag atctttttac ctaattttat ttcattcata cttaagtcct ttccgggagc
     6481 gctgtgcttt gtgctgcggc gtttcctttt aatgtgactt gattataaca cttttttgcc
     6541 cgtttgtcta tagtttgatt ggaaatttaa caaaaatgca acaaaatatt tttctcagtt
     6601 gtgcaatttg acgaaaaatg tagtaggaga atttacagta atttttcacc attatgatat
     6661 cacgtttggc tttagtatgc tgtctgtgat tacatcagtt cgtcgctgtc gaggattatc
     6721 gtgaacgggc cgtcattcag aagctcgacc ttcatatccg caccgaacct gcctgtctgc
     6781 acatcggcta tgctttcgca ccgtctgcac tgctctgtta tgtactcgta cagctcgttt
     6841 gccatatcgg gtgaacccgc accggtaaag gaaggacggt atcccttgcg gcaatcggca
     6901 tagagggtga actgcgaaat gagcagaagt tctcccccaa catctgccag tgaaaggttg
     6961 gtcttgccat tctcatcgtc aaagatgcga agccccgtca tcttgcggat aagcttgtcg
     7021 gcgatttcct ttgtatcggt ctgtgagatg ccgataagca ccataaaacc cttgccgatt
     7081 tttccaatta cctcgccgtc tacggtgacg cttgcgtggt tcacacgctg tattacaaat
     7141 ttcatatgaa aatcctttca aaagcgcata aataatgccg ccgagagtac ggcggcatta
     7201 taaaccgatc gctgtatttt ggtatagacc gtggtttgct taataagctc cgatatatct
     7261 cttgagatct tcggggttct ggcaacggct tcttgcaacc tcttccgtaa tttttcccgt
     7321 gcggacaagt cttgcaagat cctggtcgag cgagaacata ccgagagcct tagaggtctg
     7381 gatagagttc gggatctgga atatcttacc ttcacggata agcgagctga tagcgtctgt
     7441 acagctcata agctcgaggg cggcgatacg acccttgccg tcagctgtgg gaacgagcgt
     7501 ctgtgaaata acggctacca gggagttggc aagctgtgct ctgatctgac cctgtgagtg
     7561 agggggatat acgtctatga tacggtcgat ggtttctgcc gcgcctgtcg tatgcagtgt
     7621 agaaagcaca aggtgacctg tttcggcggc ggttactgcg gcgttgatcg tttcaaagtc
     7681 acgcatttct ccgacgagga taacatcggg gtcttcacgg agtgcggcac gcagtgagcc
     7741 tgcaaagctg tctacatcgg caccgatctc acgctggttt accatcgact gcttatgtgt
     7801 gtgaagatac tcgatagggt cttcgactgt gatgatatgg cagttcttgt tgcggttgat
     7861 atggtcgatc atagccgcaa gcgtcgtcga cttacctgaa cctgtaggac ctgttaccag
     7921 cacaagacct ctcgggcgga gcgcaaactc gcccattagt ccggggagca taagatcctg
     7981 caatgtaggg atatcgttac gcagaagtcg cattgctatc gcgtgatagc ccctctgtct
     8041 gtacacgttt actctgtgtc tgtgtcccga tgcggataca tatgaaaagt cggtgtcaag
     8101 acctttctga atagacgctt tgtgcttcat ggatgttatc tgatttatta tattgactat
     8161 gataggctct gtgcaatcgg gataaccgct aagctttttg atactgccgt gcagacgtac
     8221 aacgggaggg agccccgcag taaaatgtaa gtccgagcaa ccgagtcttc ttgcttcgtc
     8281 aagtattttg ttaatatcca cctttgtatt ctccttttaa aaagatatat tgctaacacc
     8341 gcatccgcat taaacggagt aggtggcttt taccagttca tcaagcgttg tagtgcctgc
     8401 aagcaccata tcggtaacgt tgctgcgaag cagctttgtt ccgttctttt cggcctgcgc
     8461 gcctatctgc tcggctgttg cgccgcttga gataagctcc tttatatcgt ttgagcttac
     8521 tattatctca tgtatagcgg ttctgccctt atagcccgtg ttgtggcatg agcggcatcc
     8581 gccgtaatga gccttgcata tttcgaccgg ctctgtcttt ccgacaagtc ttaagtcagc
     8641 gggatcggta agcattacct tctccttgca ctcggggcaa agcagcttta caagtcgctg
     8701 agctataact ccgacaagag atgacgcaac catgtaaggt gcaacaccca tatcgacaag
     8761 acggaggatc gtacctgcag catcgtttgt atggagggtc gaaagcacaa ggtgacctgt
     8821 gatagcggca cggatagcga tatcagccgt ttcaccgtca cggatctcac cgaccattac
     8881 gatatccggg tcctgacgga ggatagaacg cagagccgcc gcgaatgtaa gtcctgcctt
     8941 tgcgtttatc tgcacctgat ttacgccgtc tatcttcttt tcgcaagggt cctcaacggt
     9001 aacgatatta acgttaggct ttgaaagctc accgagcgtt gcgtacagtg ttgttgactt
     9061 acctgaacct gtaggtcctg taacgagcat aacgccgtgg gggcaacgga ttatctgctt
     9121 gaacatctca tagttataca gcgtcatacc aaggtcggtt atctttcttg tcttttcatc
     9181 tgccgttgaa agcagacgga taacgcactt ttcaccgtaa acggtgggaa tggtggatac
     9241 acggacatcg aggtttacgc cgtctatcgt cataccgaaa cgaccgtcaa gcggtattct
     9301 cttttcggca atgttcatac cgccgagtat ctttatacgg gttataagcg agttatgagc
     9361 ggcaggcttt accttcatct gctcgataag ctcaccgtca actctgagtc ttattcttgt
     9421 tctgtccttg aacggctcga tatggatatc cgatgcgttc attctgaatg aggtttcgac
     9481 tatcgtattt acaagcttaa cgataggtgc gttatcaaca cgctcgcccg actcggggtc
     9541 gtcctggata acagtgtccg cagcactgtt gtactcttta ttgaggttat ccataacgtt
     9601 gcttacggtc tgctgtgaat acgccttgtt tatcgtttcg ttgatagctg tacgggtagc
     9661 tatcattgcg tgtatctcca taccggagat aaccttgagt tcctcaaata tgtagaagtt
     9721 gataggatca tctgttgcaa ctgtaaggcg tgagtcggtc atctggaatg caacgcaaca
     9781 gttcttcttt gctatagcct cgggtatctt ctttaccgcc tcgatatcga tctttgttgt
     9841 cgtcagatcg atgaacggta ctttaagacg gatagacagt gcctgtgcaa gctgtgtttc
     9901 agaaacatag cccagctcca gcaacacgtc accgagcttt ttgcccggtt cggtacgttg
     9961 tttttcgaga gcttcgttca actgttcctc tttcagaaat ccgttctcaa caagaatctg
    10021 tcctatcggt atgttcttca ttaaactgtt ccccctgtga ttagtggtgt gcttgccgtc
    10081 tgtacttgaa aaaggcggcg atatgtggta aaatatcctt acgctgtttt acagcgttcc
    10141 aaaaaggaaa ggatggttat atatatgaca ggtatacttt ggtatgatac cgttatgtgc
    10201 atacttgtat ttgtcttcgg ttctgtcata ggcagttttc tgaatgtggt gatataccgc
    10261 actcctctgc atatgtctat cgtaaacggt ccttctcact gcttctcatg cggagagagg
    10321 ataaaaccgt acgatcttgt gccgatattc agctggatca ttttaggcgg aaaatgccgt
    10381 aaatgcaaag cgccgatctc ggtacgctac actatagtgg aggcgctcac aggatttatg
    10441 ttcctgctcg cctacataag attttcggca agtctgccga tggtggtcgc aatcgtattc
    10501 ttctcgctgc tgatagtcct cagctgtatt gatatcgacc atatggagat accctattgg
    10561 tgtactatat caattgctgt actcggtatc gccacgttct tcaccgagcc taatatgccc
    10621 tggtgggagc attttgcggg tgcggctgtt atcgcagttc cgtttgctat actcgcttta
    10681 ttcggcggta tgggcggcgg cgatgttcag cttatggcgg cagccggttt tgtgcttggc
    10741 tggaagatag ttccctcagc tgttatcggt gttgtggtgg gtgctgtcta cgggctaatc
    10801 gtgctttgcg tatcgtcacg gttcacaaag gagcattccg caaaaatcag cgaaaagctc
    10861 accgggtggt gtgagggcaa gactgtcgac agctcaagag atgttatcat aggtgaattt
    10921 gaacacggca agtgcaagat cgaccctgag ctttttgaag aaaaagcgtg gaatatttcg
    10981 ggcgatgagc ttaaagccgc aactgagaca ctcggcaacg aactgaacga agtaatgggc
    11041 gggcttcccg acagcaagga atatgtattc cgtgcaaacg tggaaaacgg caagataaca
    11101 aagataaagc tgcgccgccg tatcgctttc ggtcctgcac tgagcgtagg acttgcagtc
    11161 ggtatgctgt tcggtgaaac cattatcaag gcttatcttt caatgctgta aaataagcgt
    11221 ttatatttcc cccgccgatt aaggcggggg attttttgtt atcagccgaa aatctgtatt
    11281 ctgtagagaa ttactatatt cagatcgtcc ggataagtgt aatctgccaa ttcagaatca
    11341 cttgacggtg tcagcttaag aagcgacata ttaagcaact tgaacatctg aactcgctcg
    11401 cttaccttta tgtttccgtc agtgtcatag gtattgacac ccatacgaca ccatgcgccc
    11461 ttgtcgtttg atgtggtatt gaacgtgaac ttgtaacggg agtcattgta gtactcactg
    11521 ttgaacacag cgtaagcatt gctgtttacc attgctaccg ccttatcctt atagtctgca
    11581 acactgctga gcttaccgag atcatacagc tgatagccct ctgcagtgga gcgcaatacc
    11641 aatccgtaaa cagtttcttt gggatcctta tcggtaaaca taacattaac catctttgct
    11701 acactgccgt caacgtccga gttcttagtg agagcatcgg tcttgtagcc ttttatgcta
    11761 taagtagtag cgttcttcat cttatcggta acatagtttg ttatggtatc ggttacggca
    11821 ttggtgacag tatcctcgtc aagtgatttt acgatactgc tcaccgggtt gaacatcgac
    11881 attgccgcaa ctatcagtat taccatgatc gacattgcga ttatgacctc gatcagcgtg
    11941 aacgcctttt tctttaagag ctttctcatg gagtttcatc ctcctttaca ggtgttccgt
    12001 aaaggtgatt gtagataccg ttggtcgccg catcatcgaa tgaagccgat gtatctgtgc
    12061 ttgtgctctt cgggtctata aagtatttcg caaaagagcc gtaatcgctt tcaaacttat
    12121 cctcagggac agtgaatgtt atcgtatttg tatacgagct gtcagaatca ttggcttttt
    12181 ttgcgctgaa acgtacattt gtaccggata ccatactgta cagcatcttg gcatcgggag
    12241 taactactat gttttttgcg ctttcgggaa gtgcaatttt cagcgatttg ctttcagagc
    12301 cgttgagagc gtggtttgtt gtggttgtga tatcaaaact gagcttaatt ctgtaaacat
    12361 cgccgtcaag ctcacagctt tcttccttgg cgtatatttt ttcgataccc tttgtgccat
    12421 agatatggat atcatcggtt accataccgc cgctgttttt atccttatcc ttattgccgc
    12481 cgtcattctt gatggtagcg tcaattgtat tgatctcaag tctgccgtca ttatcggctt
    12541 cggtgctgtc cttgcgtatc ttgacatcgt tgatctgtaa attgtttgac gcgcctgtgg
    12601 ggctgacaaa cttcatcaca agcttgaccg tatctctttc aaccagcgcg ttttcctgaa
    12661 cgagattact tatctgcgtg tccatatcct tatccacgct gtcgttcttc ctgtgctgtg
    12721 ctatcgcaat agcaagtatc tgcattacga tagcggacat aagagcgaat atgactatgg
    12781 atacaagaac ctctgtcagc gtaaagcctt ttttactttt aaagcgttta aacagcttca
    12841 tgctatccgc tcctttcatc agttataacc gtactttctc catgaccttg agcttgatga
    12901 accaagcgtt ccgatctcac cgccggagtt ggatatcagc ttttccatat agtcaaagcg
    12961 tgcttcttcc ttttcatcat cgctcagtgt gctgtcgaca aatctgtcgt agtagtcata
    13021 ggggatagta gccatataca ctcttgaagt attcggcatc tcatagtcgg atacgataag
    13081 accgccgagc atcgcaaaat gtccttctgt tgacttacct gcaccaaacg tcatataagg
    13141 tgcatatata aatccacaga atgtattata tggcatatca aaattgacat cgctgttctt
    13201 gtttatcgca accacgaaca ggttattatg aattccgcct gagttcttgt acttatcaag
    13261 aatgttcttt ttaaacgcag tacgcaattc cggtttcgga tcggtcgttc gcattatagc
    13321 ttcaaccttt tctctggaga acttatctgt attggtgacc ttaccgtccg atgctatcgc
    13381 atcgggaagg atctctttaa gcagattgta gtgacccata aaaagacgtt gctgtgcaaa
    13441 atatgttaca ccgtcaccta taacgaatat tacactgccc ttacccttgg taagtacgct
    13501 gaaccaaccg gcaaactgac cttcgggctg attgttccag cggaagcagt tgaacttgtt
    13561 tttgtcctct gatttgttgg agaacttata tgtcggagcg ctccattcgt tggcaggagg
    13621 tatttcctgc atatagcagt ttgcggggag gtatacatac atatcgatat cgttgccgcc
    13681 gggcatagtg gtatcgaata ctatgttgta tccgtcccat gttcctgccg taatgcgcat
    13741 atcgcccatt ataacatact gaccgaactt ctctgcgctt acgataagct gatttttatt
    13801 gcttcctgtg cctgcaaatc ttgcgtcctg taccgcgggg tcggtagcat tgtcgaactc
    13861 gcagtttatc ggatctacgg tatacgtacc gtcatcattg ataaacttct tggtcatatt
    13921 ccagtttgta tattcagggt tgccgacttt ttcgtttatc ttcgccttga cattggtcag
    13981 agattcacaa atgccgctgt ctgagggcca tacatagttg tatgccgacg taccgcctgc
    14041 aagtgaacga gccttatcca tatttgcacg cagtgcattg tacgcgctgt tgttctcgcc
    14101 gtttttcgct tcaaaacgta ttgaagcatc ggaatattcc tttattctgc tgtcatcaac
    14161 gccgtgtgac tttatccatg tcttctgtgc accgaaatag cttgagcctg aacagaggta
    14221 gacattaccg ccgatcgtta ttccgccggg gaacttatcg ccggtgttta cctctttgtc
    14281 gatgtaaaca tcaccgttta caaaaacctt ggccgtatat gtttcgttac cgaagaatgc
    14341 ggcatcgccg ttgacatata gttcgcagtt gctgtcatcg ctgggtcgtc cgcttatgaa
    14401 atcaccgaca acataaacat cggtatcgtt cttgtagttg aacgatgtac cctgtatcag
    14461 gtttccaccg actcttatac gtccgccgtt caggttcatt ccagcctgac cgccgttata
    14521 cgtcataata aggttgttgc cgactgttat atcaaacgtc ttgtcaccgt ctgcgtttat
    14581 aggagcattg tttatcgaca gtgttcctgc gctgatgacc tcggctttta tattgatacc
    14641 tgagttaccg gactgcgaag agcctatctg gctgtactcg ttatcaagat acatagttga
    14701 ggttatttcc atacccgaca ttacaacgtc attcggtgca taacccgttg atgtgaaaaa
    14761 tctgtcgaat gaatacggct ttgaaaccag tgtgaactca cctattgagg tcactaccga
    14821 ggactcgccg tttaccacta cttcggtttc gacctttata gtctgctttt catcgccgct
    14881 gtcgccgctg agctttgtga ccgtcacctt gtaatcgcca agacctccgg caagctctgc
    14941 gaaggtatct gtgccgtcac cctttgtggt gagggactta ttgggtgaca gcgcgattat
    15001 agcatctacg aacgcatcgt ccgtctttgt gttgagatat tcggttacga ggtcgtttaa
    15061 cgatacggca ctctgatatg actgttcgtt gccgtatttt atctcgacct gtgcacgagc
    15121 ggaaatcgtg gaataataaa ccgcagatgc aagcactatc ataaccgtca ttatagcgat
    15181 aatgaagaat aaaacgctgc ccttgccgcc tttcagccgc aataacgccc ttttcatcat
    15241 taacgctttt ctgttatgtt tgttcatagc gtttcatcct ttcatttctt acttgtaagc
    15301 aaccgtttat tcggctgtgc tgttgttagc cgcaaaggga tctgctacgg gctgtatgca
    15361 gagcaggttt acggagaact ccatcgtgta atcgcctgcc gatgtgtttt cacccgactc
    15421 gccgcctgta gcagcgccgg cttccatctg gtagctgagt gacgctatgg aaactgcctt
    15481 gctcttgccg ttcttttcgt atacgcccga gtaaagcaga tctgcaaagt tgtgcagatt
    15541 ttcaagcttg tttgaatgag ctgtaaacga tactgttaca gagcctaccg ttacgggttc
    15601 gcttgccgca agcaatgtag cctggagctt ctttgcgtac ataacaagct ctctgggagt
    15661 taattttgac agatctgtct gagtatccga tggcgtttcg acaacggtat tcgcaaagtc
    15721 cttaagagga tatgtgacca ctgtaggagt gaataccgat acggaaagcg cctgcgttga
    15781 gaagggagat atcgtcagac cgtcaatcgt tatatccttg ccctgagaaa tgaacttacg
    15841 cataatttca tcggcttcgt attctgtgag gtcgttgaag aacttgtctg caaggttctt
    15901 accgtcctcg taagcctgac ttatttctgt gtcgatcgtg gtttcatgtt cgagctttgt
    15961 cagtatgtca gagtattctg cctttaccga tgtaagcttc ttgttgtttt cgcctatctt
    16021 attgaagttg ggaagtataa atacaaatat tcccacgccg atgatcgcgg ctactaaaaa
    16081 tacaactata aggattcgtt cctgtttact gatcttcata ttcgtttacc ctttcccgtt
    16141 actgtgccga tgcttcggag gtggattctg tcgaggactc ggcggatgag gtgttatcct
    16201 gtgcgccctg atcgccgttt tcattgttct cgattatgct gttcgcagaa tccttatcga
    16261 tcgtaatgtc attgcctgcc ttgagtaaca tcgaaaggct gaacgtaact gtttccttgt
    16321 tatcctcgcc cttttcgacc tcataaccgg tatatgagat gttctcgaaa tagccctgag
    16381 ctatcagcgc tcttacatac tgtgaaggct gcgcctgatt ggtacaggta aatgttgccg
    16441 aaacggtata tcccgatatt tcgaccgtat ctataagctt cataccgtta aggttcttgc
    16501 ggatatcatc gcttgtaaca ttttcgattt tatcctgctt tgctgtgaac ggttctctga
    16561 gcatcttata tacggttgtc gtacccttgg gcatatagtt atacatcagc tccgcatttg
    16621 cgatggagtt tttgtagctc tggaagtttt cgagtacgcc ttccttctgc tgtaaggtcg
    16681 taagtcttgc ctgaagctct gtgtcgttta tctgagcctg tacctttgtt atctcgccct
    16741 ttatgctgtt gttgataaca tctacaacaa catttgcgcc gatcactgca atcacactca
    16801 atgctgccgc tacgccaaga cctataaaga acgtattaag acccttgctc tcccttgcgg
    16861 ctgtcgttgc aaggaggttg atgtgctcga gctctttatt gactttatag agagcgccga
    16921 tcgcgtttgc atagactgta aattcaaagt ctgttcttga agctatagtt gtgggtgcgc
    16981 tgagtatgtg acacggtacg ttgaagccgg atacggtatt tgtaagtgct ataaagtcac
    17041 ctatctcgcc gtagaacatc atttccttta tgggcttcat atccttcctt gactggaaga
    17101 actggaacat tctgaacagg ttatcataga tagcctgatt tacatagtcc tctgcgttat
    17161 tatagtcagc gggatcgatc ttgaaatatc ttgagaatga aagaacattg tcttcataaa
    17221 ggttgatatt caggaactcc ttatcgatct gcaccagtaa gagcggcata tagcctgaca
    17281 tcttaggtgt gttctggata agacgcgaga tggcgttgtt tgaaatatta acagccttca
    17341 gcgaaagacc catatgagtg aacagcttta catagccgtc aacaagacgc tgggggcagg
    17401 ctgtcgcaag gaccnnnnnn nnnncgtgat gagcgggttc ttgttctcgt ctactgtttc
    17461 acctactata gtatatgaaa tgttgtagtc gcccgagata cccatattgc ttcctatcat
    17521 ggcctcgatg gcggccgtgt ttgaaagctt cttgggcttg gggagcataa gttccttata
    17581 gagtatcgag ctagagctta tcgatacaat ggtttctttt tcctttatcg ctctggtatt
    17641 gatcatatcg attatttctg ccgcaaccag cgggacatct gctatgtagc cgtttgaaac
    17701 aagacctttt tcgatctctc tgaaatcaac ttcatcgatc tttatctttg ctccctgtgc
    17761 cgcgccgcgg acgagcttga tttgtctgtc ggtaatatca atagataaca tatatcaaaa
    17821 attccttcct gttaattagg ttgagttttc gctgctcttt aagtattgcc cataccttcc
    17881 atacctgagt aaaggatcgg gaatacacct gcaagtaagc aacctaccat aatacccata
    17941 agtatgatca tgacaggctc cataagacct acaagtcgtg aaattgccga gtcggcttca
    18001 tcgtcatagt aatccgatga ctttgcaagt atcgtatcaa gagtacctga ttcctcaccg
    18061 acatagacga tggatacgaa cataccgtca aatac
//
LOCUS       DAWAOG010000002        16567 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_702_length_16567_cov_2.340480, whole genome shotgun sequence.
ACCESSION   DAWAOG010000002 DAWAOG010000000
VERSION     DAWAOG010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 16567)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16567)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16567
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_702_length_16567_cov_2.340480"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    256..265
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1355..1364
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6106..6115
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11030..11039
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13077..13135
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13477..13486
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13966..13975
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4522 a         3739 c         3750 g         4437 t
ORIGIN      
        1 tggcctatgc ggtaaggtcc tctgcactca atgtcctggt ttattgcgat aacttccttg
       61 ttagtcagta tctcctttgt cctgtcggtc atatgacgga tatcacagcc tatcataaga
      121 ggagagttca tcattgccca cagtgagaaa tgcgtcttgt actccgtgtc ggtacagccg
      181 ccaagaccct tgccttcctt gttgccgaac tggtcgtcgc tctcgccgtt tatgtactgg
      241 ttgtttgagc cgccgnnnnn nnnnnccgcc gtgcataccg acaacgagca tatctatatc
      301 attgtggcag aagcagccgc catagcattc gttacccatc tgtgacagcg caagacgctt
      361 tatcgactcc cagttatcct gaatatcgcc tgtcgagcgg aaaagatgag cgccgctttc
      421 tcttatccac ttgtatatat cgtcattgcc ccagttgcaa gcggagaaca ttatcggtct
      481 gccgcagttt ctgagagctg tcgacatacg cttgtagagt atctcgccgg gtatatgtac
      541 gggcttgtag cagtagtcgt atttcagata atcaacgccc cactcggcga atgtttctgc
      601 gtcctgaaat tcatgctcaa agcttccggg gtgacctgca caggtatgcg tgcccgcaca
      661 cgaatagata ccgaacttaa gaccctttga gtgtacatag tccgcaacag gcttgatacc
      721 gttcggaaac ttccaatgat cgggtacaag ttcgcccttt tcgtttctct gcttttcgct
      781 ccagcagtcg tcaattacaa cgtattcata gcctgcgtcc ttgagtccgt tttcgatgaa
      841 tgcgtcagcc gcctgcttta taagctcttc gtttatctcc caggtaaacg tgttccagct
      901 gttccagccc atagggggtg tcattccgag taacttttcc atttttgttt tcctttcaga
      961 tattattctt caaagctgtc ctcttcgttt cttgtgttcc acagctcgac caggcgctcc
     1021 atttcttctt ctgtgagcga aatgcccttg cccattctgc tgtggtcggg tgcccatgaa
     1081 cggatatcgt acttaggctc tctgtcgttc cagcttacca tattaagctc aatgttgtac
     1141 ttgccctcgc ttataacgcc gagctcctgt gttatctcat acttgaattc tgccatgtcc
     1201 ttagttcctt tcttatatac ggagttttta atttttatat taaagaggac ttattcctct
     1261 tctcctgcgt ttttatcctt ttttgacttc ttttcgggtt tttctttctt tgccttttcg
     1321 ggcttctgct ttttctgctt gggaagcctt gtatnnnnnn nnnngccttg tatcgtccgc
     1381 aagctctacc gcatcaaata tgcaacggta tcggggcggt atcagcttgt tgatatgcct
     1441 tttgccgaaa aaccacattc tgaactttgc cgtatcaagc accttgtcaa gatatgtatt
     1501 tatatgattg cggctggttg tgcctatgtc gatgaactct atcattcttg ccggcggctc
     1561 atagctgatt ataaaatccg cctcgttgcc gtgctgttca aggtcgtgaa gcccttcttc
     1621 aagctcctca tcggtcggaa gctcctttac ccacttctgc tcctcatccg gttcgagata
     1681 gctgttgttc tcctcgctct gaccgccgcc gaaagcaaat accgtttttt cggcgatcgt
     1741 gtagtactga cctctcatca gctgacgcag gttgcccgat atgagccttg tcttaccacc
     1801 gcaccactct tctaccgggt actcgctcag taaatcgaaa ttctcgtgta cgccctcgac
     1861 aaaaagtatg ttatatttct tcttgccgag ctttttgagc atactctttt cttttttact
     1921 gccgtcccat atacagccga agtcgcctgt tattatcagc gcgtcaccgc gtctgagctt
     1981 tttaagtatc ggcgctttaa atcgtgaaat atcgccgtgc aggtcgcctg taacgcatat
     2041 cataagctat atccttcttc cgtttttatt atccttactt acgttaagtg tatactccag
     2101 tctatcggct ctatgccgtt ttgctgtaaa aaagcattta cctttgagaa atggcggcaa
     2161 ccgaaaaatc cgttatacgc cgacagcggg cttgggtgtg ccgctgtgaa taccgcgtgc
     2221 ttcggatttg taataagcgg cattttcgac tttgcgttgc ttccccacag tatgaatgcc
     2281 gtaggcacat cgcgctcgtt cagaagctca attatcctgt cggtgagtat ctcccagcct
     2341 ttccccctgt ggcttcccgc cataccctca cggactgtaa gcgaggtgtt cagcatgaga
     2401 actccctgcc ttgcccagtc ggtaagctcg cccactgaca tatcaaagtc tatcccgagg
     2461 tcgttatgta tctcggtaaa gatatttttc agcgaggggg gcggcataac tccgcgcttt
     2521 accgaaaagc aaagtccgtg cgcctgtccc gcaccgtggt acggatcctg acctaagagc
     2581 accgccttta cctttgagta cggtgtatat ttcagtgcat tgaaaatgca gtccgcaggt
     2641 gggtatacat tgtatctgtt atattcatcg acaagaaaag ctctcagctt cttgtaatat
     2701 tcacttcgga actgatctgc aagcagctcg tcccattcgt tgcctatatt gaccattttg
     2761 tttctccgtc agatctcgtg tgttcttgca tagtcgaata aatactgccg tcgttgccct
     2821 ttatacattc cggcagaccg tccggatata atttctgctc gatacgcttt atccatacat
     2881 ctctcggaaa agcgtccgtc ttgtgatagc cgaaaagcag tacgcagtcc gcaaccttgt
     2941 ttccgacacc ctttatcttt ttcagctctt ctcttgctgt atcggtatcc atagcgttta
     3001 tgccgtcaag cgaaacctct gtgctattca gcttttctac cgcgtcaact atgtattttg
     3061 ctctgaagcc cgctctcagc ggtgctaagt cctcggcggt aacgccgaca agcttttcaa
     3121 gtgtcgggaa agcatatccg tgctccgtct tttccccgaa gctctcgcac agcctgtcaa
     3181 taataccggt gatacgcttt atattattat tctgcgagat tataaagctg atgagcgttt
     3241 caaaaggctc ttgccttagt actcttatac cggggttttc tttgcaggca aggcgcatat
     3301 gctcgtcctt tgagaaccgc tctatcagtg cgtcgtagtc tgtatcaagg tcaaagtatg
     3361 actgccagta agaaatatct tctttttcta tctcgtgtat ggtgtaagtg ttgtcgccgt
     3421 tgtcggttat gccggcgtat ttaccgcccg ctatgccgca gaatctgccg tcaagctctt
     3481 tccagcggaa agcctgtccg cagaaaagcg tgcgccttat atcaaagccc ttgcaagtga
     3541 atgtgtagtc catatgatca tctgccgtta tccgtttttt gaattatccg tttttgtcgg
     3601 gggaaaattt taaatttagt cttgattatt tccgttatat ataatacaat attaacagag
     3661 aaattacaag accgacatga aaaatttttc ttttgtccct cttataaacc gacacggatt
     3721 ttaaattctg cggtgttttc cgcatatatc aaattttttg aaaggaaacg aaaaaagtga
     3781 gagaaagcat actgactata ccggtaaacg aagtatttga gccccgtgag ggctgtccta
     3841 tctgccgtat gagagatacg gtggaggagc atatatgcga atatataatg ggggcggcta
     3901 tgatggagcc tgacgtaagg caggatacca acaggctcgg cttctgcttt acacactatc
     3961 agcagctgat gatgcagaac aaccgcttgt cactcggact tatgctcaac agtcatcttg
     4021 aagtgctcag aggtaatatt tttgaaaaga aaggcttgtt cgctcctaag gatgcaaaag
     4081 ctaaaaaagc aggtgcggta ggcgatacct gctttgtctg ctcaaaggta cagtggggcg
     4141 ttgaccatat gcttgaaacg gtattcacaa tgtttgccaa ggacggcaag ttcaaaaacc
     4201 tgttcaagtc gcaggaatgc ttctgtatgc cgcattatca ctatcttgta cagctgagcg
     4261 aaacgaagat ggcaaagaac gacagggccg agtttgtaaa ggtaatggat acgataatgc
     4321 aggaatatat tacaaagctc aacagcgatg taaacgactt ctgcaacagc tttgattacc
     4381 gtaacgccgg caagctccac agcgaagaga tggaacacgt gcgtacatct atcgaaagga
     4441 gcgtttcgtt ccttacggga agaaagccga catcgaaata aatctcatta tacgttagta
     4501 ttttaatatt taacattaca tcacagttca gcgatataat ccgaaaaacc tcaaaaaccg
     4561 cttgaaattt ttgaaggtaa gttgtataat gaagtataaa ccacttttac ggaggtgtcc
     4621 atgagcaaaa aaaacacagc ggctaccatt gctacaataa tcgttgccgt gcttgcaata
     4681 gcaacgactt tgttcctgct gtaccagact tcacaacagc agatacagga aaatcaatat
     4741 aattacattc cctcggacga ggtcaacgaa gaaatgaata tgaatgcggt aactcttatc
     4801 aagaacaact gcgagatatt cagaatttat cttcagtatg gtcttccgca tcaggcagag
     4861 ccgtataaca atgttcccga gaacggctat tataccgtaa agagcgacag ctacaagaca
     4921 atgtctgata ttgaaaagct tgtcaattca acatttgttg aaaaagaagc aaagcgtatc
     4981 ctcacagaca taaacggcga caatatcgct gtctatgccg aagaaacaga tgaggacggc
     5041 aacaagggca tcggtcttga tatgaatatg gttgacgaga acggcagatt taaggctctt
     5101 gactacggct acacatggtc aaacgccagg ttcacacttc accccaagtc aaacaccgag
     5161 tgcgatataa tcgttgagct caactctgcg gaggaaacct cctcagccga tacaacctct
     5221 tccggctcgg aaagcagcat caaaaaggtc accgccaata tgctcaaggt aaacggcgag
     5281 tggcggcttc agaagctggt ttattgataa atgagtaaaa aggacaatcc tcttaaagtc
     5341 tatgctgttg taagtcagat aggttttctg gtgataactc cgctgctgat attcatatgg
     5401 ggcggcagtg tactggcaga caagctttcg cttccgtcat gggttatgat cctgcttgtg
     5461 tttgcaggga taatcactat gatatcggga gtggggactt atcttaagaa ccttataaaa
     5521 atgtacggcg gaaaagacga caagaaaacc tataagtatc tttccgaccg ccgtgacaac
     5581 gactattacg acgataaccg ccgtgacagg aagctttaaa tatgaaaaag aaaattaccg
     5641 aaacgacatc attcaaggaa tttatggcgc tgttgccgta ttatatagga gcgcttgtat
     5701 tgcttgggat agtcagcgtt atcctgatga tttcgggaat aggcgactac acactgctta
     5761 cgggtgcggc agtcggtaca gcggtttcgg cactgaattt tgcgcttatg gcaatgagtg
     5821 cggaaaaagc cgttacgatg accgaaaaat ccgcaaagct tgctatgaac ggcagttacg
     5881 gtgtaagata tatctgcaca ttcataattc tcggtgtgct gatgtttttt aaaatcgtca
     5941 atcccgtcac agctgtactt ccgctgtttg tgccgaaaat cggctacacg gttaccgctt
     6001 tcaaggagaa aagcgatttc tgacagcaga atttacaatc gctgacttaa cattaaattt
     6061 atataaagat ataaaatatt cattacggga acgacccgta aaaatnnnnn nnnnnatgac
     6121 agaccttata aaacctatgg cggaattttc agtagaagga ccgcttaaat cgataagctt
     6181 cgatataggc agctacaatg tggtaatcag cgagagtatc gttgtacagt ggatagttat
     6241 gcttgtcctc ggcatcgtat tcttcatact cggcagaaac ctcaaggtga ggtcgacctc
     6301 acgcagacag atggcggctg agtatctggt aaacttcttc aacaatatgg tgcgtgacac
     6361 catgggcgaa aaatacagaa agtacacccc ctatataggc ggacttttct gcttctctat
     6421 tctttcgagc cttatgggac ttttcggctt cagatgtccg acctcggacc tttccgtaat
     6481 agccgcatgg ggcatcacga cattcgttct gacgctcagg aacaagctga aaacaggcgg
     6541 tatcaagggc tatttaaagg gctttatcga gcctatgcct tttatgcttc cgttcaatat
     6601 aatcggtgat attgcaaacc ccgtttcaca gacactgcgt cactttgcaa atatcctcgc
     6661 aggctctgta atcggcggac ttatctactt tgctttagga caggtcgcaa acggacttgc
     6721 atctatcggc gttcccgctg tatgctcgct gtactttgac ttgttctcgg cattcattca
     6781 ggcttatatc ttctgcacac tgacaatggc ttacgtttca atggcagaat gcggagatga
     6841 ttaaacagtt tacccccaca ggaaactgtg gtgtaataaa aacaataatc tacctacagg
     6901 tagaataaaa tgacggagga ctttgatatg gaaaacttat cacaggctat cgtactgggc
     6961 gcatctgcaa taggtgcagg tcttgctatg atcgcaggtc ttggccccgg tatcggtgaa
     7021 ggcttctgcg gcggtaaggc tgttgaagcg atcggcagac agcccgaggc ttcgggcgca
     7081 atcacgagaa caatgatcat cggtgacgct cttgcggaaa ccacaggtct ttactcactc
     7141 gttatcgctc tgctgctgat gttcgcaaat ccctttatca gtcagcttta agttctggca
     7201 gactacaaca cgaaaggatt gaagtgaaat gacggatatg ctgagaatag ccgaggaagc
     7261 ggctgcgcag gcaactgaac aggctaagtt ttctctggtc agcatagatc ttaacactat
     7321 agtcttcact ctgatcaaca cgctcattat cgttctgctt tatttcttct tcttgcacaa
     7381 gcccgtctgc aaaatacttg acgagcgcgc gaagacggta aacaaggata tggacgacgc
     7441 acagaaggca aaggaagagg ctgctgcagt aaaggcagac tacgaacagc gtcttgaaac
     7501 atcaaaggaa gaggcggccc gcattgtagc cgacgctaca aagaaggcgc aggcaagaga
     7561 ggacgagatc atctctgccg caaacgaaga agccgcttct atgaaacagc gtgcggaaga
     7621 atctatcgag cgtgaaaaga agcgtgcggt aaacgagata aaggaagaaa tttccgatat
     7681 ggtagttatg gcggcttcaa aggttgccga aaaggagata aacgcgcagg acaacgagaa
     7741 gataatcgac tctgttctgt cgcagatcgg aaaggagagc tgaaatggct gctgttgaca
     7801 agacatacgg cgacgcattg ttctctctaa taaccgaaga ggatgaaaag gcactgcccg
     7861 acattctcgg acagcttaag ggcgtgtcgg aggtttttgc cgccgagccc gattttataa
     7921 agctgcttcg tactcctact atccttatag aggaaaagac agggcttata aaagaagtct
     7981 ttgatggcaa ggtgcacagc ttcgtgctta actttctgtg catacttacc gaaaatgaca
     8041 gactgcccga ctttgaacgg atactggcat actttacagg gctgtataac gaaaagctcg
     8101 gtatagcaga tgttacggta acctctgcaa tgcctctttc ggatacggct gttgaaaaga
     8161 tcagagcaaa aatggctgat atcacgggca agaccgttaa tatgacgctg aaaaccgacg
     8221 ataagctcat cggcggcgtt gttatcagct acggcaatac gactatcgac ggaagcgtca
     8281 aagcaagact tgaacagctg aaggcggata ttgccggaac tatcgactga cataaagatc
     8341 gtcagccggt aaaaaaatac gaaaggaaga acctccaatg gatttacgcc cggatgaaat
     8401 aacaggcata attaaatccc ggatcaagga ctacggctca aagatccgtc ttgaagatac
     8461 aggtactgtg gtaactgtag gtgacggtat tgttcgtata cacggacttg aaaagtgtat
     8521 gatgaacgag ctgctggaat ttgagaacgg cgttcgctgc atggcgctca acttagagca
     8581 ggactttgtc ggtgcggtta tgctgggctc agatgccgag atcaaagagg gtgatacggt
     8641 aaagcgcaca ggttcagtcg tatctgtacc cgtaggcgag gaactgctcg gacgagtagt
     8701 aaacgcactg ggtcagccta tcgacggcaa gggcgctata ctcgcaaagg aaacacgccc
     8761 gattgaaaag atcgctccag gtatcattac gagaaaatct gttaacgttc cgcttcagac
     8821 aggtatcaag gctatcgact ccatgatacc gataggaaga ggtcagcgtg agctgatcat
     8881 cggcgacaga cagacaggta agactgccat tgcgattgat acgataataa atcagaagaa
     8941 aacagacgta atttgtatat acgttgctat aggtcagaag gcttctacag ttgctaacgt
     9001 tgtagaacag ctcaccgccg caggcgctat ggactacaca atagtagtat ctgctaccgc
     9061 aagtgagctt gcgcccttac agtacattgc accctattcg ggatgcacca tgggtgaata
     9121 cttcatggaa aagggcaagg acgcacttat aatatatgat gacctgtcca agcacgctgt
     9181 ggcttacaga gcactttctc tgctcctcaa gcgtccgccc ggacgtgagg cttaccccgg
     9241 cgatgtattc tatctgcatt caagactgct cgaaagagcc gcaaacttaa acgagaacta
     9301 tggcggcggt tcactcaccg cactgcctat aatcgaaaca caggcaggcg acgtttctgc
     9361 gtatatcccg accaacgtta tttcgataac cgacggtcag atattccttg aaacagagtt
     9421 gttccactcg ggtgtaagac ccgctgtaaa ccccggtatc tcggtatccc gtgtcggcgg
     9481 taatgcgcag ataaaggcta tgaagaaggt atccggtact cttaagctcg aatattcgca
     9541 gttcagagag ctgcagagct tctcacagtt cggttcggat cttgataagg atactaagga
     9601 gcgtcttgca aagggcgagc gtatcgttga gatattaaag cagggacaga atgctccggt
     9661 agctgttgaa aatcaggtca taatcatcta tgctgtaatc aacaactatc tgaagaacat
     9721 tccgcttgaa aaggtcagcg actttgagcg tgacctctat aagcatatgg aagaggctca
     9781 ccccgatatt acagcaagca taagagaaga aaaggtactc actcccgaaa acgaagaaaa
     9841 gcttaaagcc gctctcaagg atttttcgga gaagtacgtt taaaacttaa gatataagca
     9901 ttaagcgaaa ggaagaatag ttatgaaaaa ggcatcactt gtactgagca tcgttgcttt
     9961 gagcatatcc gcactcaccc ttgcgctcac ggttttagag ctgttctata aaaagacgac
    10021 ttatatcgac agtgacaata tccgataaag gaggctttgc atggcttcgg gaaatatgaa
    10081 ggacatcaag cgcagagtaa aaagcgttga gtccactatg cagataacga aggctatgga
    10141 gcttgttgcc tcttctaagc tgagaaaggc aaagcttcgt gccgaggatg cgcgtccgtt
    10201 ctttgatgca cagtattccc ttatgtgccg catagcaggc gaaacggaga acctgagcac
    10261 cgtatataca aggcacagag aggtaaagaa ccgactgttt attgtaatag cgggcgacag
    10321 aggtcttgca ggcggttaca actccaatat actgaagctg gtacagctaa cgcacggcga
    10381 tgaatatatg cctaagataa tagctgtcgg caaaaagtct gtggagttct ttacaaagca
    10441 cgaatacgat gtagtagcgc agttccccgg cattgcggag catatcaaaa cggcagacgc
    10501 tgccgatata gcgcagatag cgacagatct tttcacaaag ggagaggtcg atgagataga
    10561 gctgttcttc acacagttcg tatcccctct tgtacagacc cctacccgta tgcccgtact
    10621 ccccatagat gcaccgacag gcaaggcagt acccgaaaac aaggcaggta cgacctatga
    10681 cccaagtcct gaggcagttt tcaaccgcgt gataccaaag cttatcacaa gtcttatcat
    10741 gtgtgcggtc aacgaaagct atgcttctga gcttggcgca agacgaactg ctatggagaa
    10801 tgcaacggac aacgcagagg aaatgatcgc aaacctgtca cttatgtaca accgtgcaag
    10861 acaggaaaag ataacgaacg agctcaatga gatcgtatcg ggtgctaatg cgcttaatta
    10921 agttaagaaa ggacaggact gatctatgcc acagaatatc ggcacggttg tgcagatcat
    10981 cggtgcggta cttgatataa agtttccccc ggagcatctg cctaacctgn nnnnnnnnnt
    11041 agaagtagcg cagcatatcg gtgacgatac ggtgcgttgc atagcaatgg gaagcacgga
    11101 cggacttgta agaggacttc cggcagtcga tacgggcgcg tcaataaagg ttcccgtagg
    11161 cagacagacc ctcggcagaa tattcaacct gctcggcgag gctgttgata acaagcccca
    11221 gcccgaaacg gaagaaaaat gggagatcca tcgtcccgct ccgtcatttg aggagcagga
    11281 gggttcaaca caggtacttg aaacgggtat caaggtcgta gaccttatcg cgccttacct
    11341 taagggcggt aagataggtc tgttcggcgg tgcaggtgtc ggtaagaccg tacttattat
    11401 ggagcttatc aacaacatcg ctaaacagca cggcggtctt tcggtattca caggtgtcgg
    11461 tgagcgtacc cgtgagggta acgatctcta ctacgagatg aaggaatccg gcgttatcga
    11521 taagacaacg cttgtttacg gtcagatgaa cgagccgccc ggagcgagaa tgagagtagc
    11581 cctctcaggt cttacaatgg ctgaatactt ccgtgataag gaaggtcagg acgtgctgct
    11641 cttcatcgat aatatattca gatttacaca ggcaggctct gaggtttcag ctctgctcgg
    11701 acgtatgccg tcagccgttg gttatcagcc tacactggcg accgaaatgg gcgctttgca
    11761 ggagcgtatc acttctacaa agaagggctc tatcacatcg gtacaggcag tttacgttcc
    11821 tgcggacgac cttaccgacc ccgcacctgc tacaacattc gcacaccttg atgcaacaac
    11881 ggtactttca agaaacatct catcaatggg tattttcccc gctgttgacc ctcttgacag
    11941 tacatcaaga atactcactc ccgagatagt cggagaagag cattacgagg ttgcaagaca
    12001 ggtacagtcg atactccagc gttataacga gcttcaggat atcatcgcta tcctcggtat
    12061 ggacgagctt gacgacaacg acaagcttac cgtatcaaga gcaagaaaga tacagcgttt
    12121 cttatctcag ccgttctcgg ttgcagaaca gttcacaggt atgaagggta agtatgttcc
    12181 gctcaaggaa acgataagag gcttcaagga gatcatcgag ggcaaacacg acgatcttcc
    12241 cgagtctgca ttcctgtttg taggcactat cgacgaggct gttgaaaagg ctaagaatga
    12301 gaaggcgtaa taacaagggt tatttcagcc ctatgacgat aaccgcgctt ttactggcgc
    12361 taattataac aggactaccg tttataatga gcggacctgt aaaggacgat gatatattga
    12421 ctccattgcc gaccccggaa acaacggcgc aggaatcatc ggtcatcgtg actacaaaaa
    12481 agcgcacaga tgtatctccc gcaccgtcag ctgcagtaaa gcccggaaac gacattccga
    12541 gcggtattat aagcggtgaa acggtaacgg acgtacagga aaaagcaaaa gcgctcgaaa
    12601 gcgttgcact ttcgctgtac agcgaaaacg gcgacagcct taccgccgat atggtaaagc
    12661 agaaagccgg cgtacccgca gatgagcatt ttatcgttac tataagcgtt aaaaacggca
    12721 acgtaagtaa gatatcgtat tttaccgagg actattccgt ttcttacgca aacggcagtt
    12781 attccgcact tgcgaatatc gccgatgaaa actttgaaga tttggtattg ttcttataac
    12841 tcccgcggaa aggaaagatg atatgacaga attcaaactg aagatcctta ccccggaggg
    12901 agtagtatat gacggtatgg ctgaaagcgt tatcgtccgt actaccgtcg gtgacaaggg
    12961 catacttgca aagcacgagc catatgttgc ggcgctgaca ataggacagg cgaagatacg
    13021 cattgacgga gctgtgcgca taggcgcagt ttcttcgggt atagtcgagg tcacaannnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcaga
    13141 catcgatact cgctcagtcg tttgaatggg cagacgaaat agatgtcgag cgtgccgaaa
    13201 aggcaaaggc tcttgctgaa gagcgtatga agcagtacaa ggacgatcag cgtgctcttg
    13261 atattgctga gtacaagctc aagagagcaa taaacagaat aaatacggct aatataaagc
    13321 tgtaagacgt ataacacgac cccctgtctg ccgggtagca gacagggggt cgtgttatgc
    13381 ttacaaagat atttgaatga ttttctctgt agttaaaaat ctaatagata agttccatca
    13441 gcgtattttc taaatgtaac atacgtttta ccgtatnnnn nnnnnngtat aattcaagca
    13501 tctgtttggt aacatatcct ccttgtggag ctccgattct tttccttatc cattcaccta
    13561 atatagagtt attttcagca ctatgtagcg ctttatttcc ctgttgtgcc atacgcatat
    13621 gaaaagaacc gaaatctttt gttattactt taaagatggg acagttttcg tcgctcggag
    13681 atagccgatc aggaaaaaag cccggcattt tatctttgga gttatacggt atataggctt
    13741 gatttggatt tcttcttgtt ccgttttggc gaatgcccca gttgatacca gaaccatatc
    13801 cggtgtcgct tccatcggct ttaagcaaag aaattttcag tgttgcaata ggcgttttta
    13861 gatcataatc ttgatattca tctcctgtat tgtcgatatc ctctatattt tcaggggaat
    13921 tatttgagat gttttttaat ttgtcaaaaa cgaggggatc aaaacnnnnn nnnnngtatc
    13981 gggcaatagg cttgagtaat aattatacgc actgatagaa tcacaaatgc tgacagattc
    14041 tctcctttta taaaaagcgt tcatagtata atttaaagaa ccgcagtatg cgatatatgg
    14101 aatttcggaa ttattattgt gctgattcca tacatatatt tttgaatgaa cttcagcgcc
    14161 ggaacaaata tacttgcagg taactttcgg catagaattt gatttattca attgctcaag
    14221 tagtctgcaa atgttttgat gttttttaac tgataaactt gatttagtca tacccaaaat
    14281 tatatcaatc gtaatgccgg ataaaaattt atcacttttt atgccatctg caagagcaat
    14341 agcatgagtt gaaattcgtt cgcaatcggt aaaagcggtg actactttaa gagagttgca
    14401 aggatgttcg ctatttgatc ttgccggtgc gtataaaaca gcttcgtcaa agttagaaga
    14461 taaaaagtta ctttggctat tagtattttg catattagtc ctcctctata ttagctttaa
    14521 tccatgggta ttcgattccg gcaaaagtgt tcaatatagc tctgaatact atatttgata
    14581 atttacaagg tacagccata ccaatttgtt ttctaacact gctgtaactt ccgaaaaact
    14641 cataattatc aggaaaagtt tgtatccttg ctctctctcg attagttagc tccctgtccg
    14701 tccaatggta cataaaagtt cctccgccac cggcggctgt aactgtataa gcgggtttct
    14761 gcggatctaa tttacgataa atctgactga ttttagtgtt tgtatgtatt ttcaaatctt
    14821 caggaaaatt tgtgcctaaa cgttcttctg cctgccatat attttcaccc ggcttaatgt
    14881 aagataaacg cttgattact tttggactga gtttcctaat ttcattattc ggcgcatttg
    14941 aaggaatgtt tgctaaagcg gttttcgatg ttatgtcaat attattataa attgaggggt
    15001 caggaactct gaattttggt tgcccgtcac gtacgccaac gataattatt ctgtgtcgtg
    15061 tttgtggtac accgtattct tctgccttat atagattgac atttagtcta tacccggcgt
    15121 tgcgcatatc gtttagtatt gtatggaaat cccctgaacc tgcagagcgt attcctgaga
    15181 cattttccgc aagaaaccat tttggttggt acatttttaa gatctctatt ccataccaat
    15241 ataattgacc gaatttctcg ttttcaaagc ctttatgttc gccaacattt gagaaactgt
    15301 tacaaggaaa gccatagcag aatgcgtcaa ttttgcctaa tgatgataaa tttaaatgcc
    15361 ttacatctga gcagtgtacc gactccggtg atttagggca tatgttgtgt atatatgttt
    15421 tgcaagtatc taaatcataa tcgttagccc atttgtgctt tatcgtaaaa catccgtcat
    15481 cactttgggc gtgtaatgca ccatatgcaa taccaccggg accgcagaaa agttctccta
    15541 attcaaattc atgtttttta ttcataaaac ctctttagaa tttttatcct tttataagcc
    15601 ccaccagtcc cataacaaac acaagcatta caagcacagg caggaaatag gtcatatagc
    15661 ctttcataaa cttctttatc ttaagaccct tgccggtatt cgcttcattt acgaagttat
    15721 cccagcccca gccctttttc gtaacacaga acagtatgaa taccaatgag ccgagcggaa
    15781 gaataaagtt gctgacgata gtatcttcaa gatcgagtat tgtacttccc gcacccagcg
    15841 gctgaaagcc gctcagtatg ttaaagccaa gacagcaggg cactgacagc acaaacagca
    15901 gtatgccgtt tataaggcag gacttctttc tgctccagcc ccacagatcc atcgtgtttg
    15961 ctatgataca ttcaaatacg gcgagtaccg ttgacagtgc cgcaaacgac ataaacacga
    16021 agaaaagact gccccagagc ctgccgagcg gcagattgtt gaatatgttg ggcagcgtta
    16081 tgaataacag cttaggtccg cttgtaacat caactccgta tgccgagcag gcggggaaga
    16141 ttataagccc cgatgctatc gcaacaaacg tatcaagcag tgccacgttt acagattctc
    16201 cgagaagaga gcgatcctta ccgatgtaac tgccgaatat agccatactt cccataccga
    16261 tactgagcgt aaagaacgct tgcttcattg cgcttacaag gatattcgtt atacctacct
    16321 tttctattgc accgaagtcg ggtagaagat agaacgaaag tccttccgct cccccgggca
    16381 gtgtaaagct gtttatcgca agcactgcca taatagcaag cagtgcgatc atcattacct
    16441 ttgtgacctt ttcaagtccg ttctgaacgc cgataaagca tacgagaacg cctatcacaa
    16501 cgacgatccc catatagcct ataagcgaaa cagggtcacc gagaacatca atattgaagt
    16561 ggttttc
//
LOCUS       DAWAOG010000003        15927 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_721_length_15927_cov_2.656880, whole genome shotgun sequence.
ACCESSION   DAWAOG010000003 DAWAOG010000000
VERSION     DAWAOG010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 15927)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15927)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15927
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_721_length_15927_cov_2.656880"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    208..217
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2291..2300
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5072..5081
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5814..5823
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8276..8285
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8882..8891
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9230..9239
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4089 a         3708 c         3436 g         4624 t
ORIGIN      
        1 attcatgaga gtcctaattg acacttcacg cttatagctt tgctcaagtc ggtaaatttc
       61 ctcatatatc tctggaaagt gcacccgcaa atcattcaga atttccaaac cgcttatgta
      121 gcagtcgaca taaattccga cgacttcatt taaagcttca tcagcgtcgt caacaagcat
      181 gatagccttg atctttctgc gtatattnnn nnnnnnnctg cgatgtgtgt atagtcagac
      241 ggtttatctt ttttggcttt aattacttct tgacgtttgc tttcagcgtg tgcaagaatg
      301 cgagtgtcct tatattctgg aatattaaaa tgccactcaa caacaggcgg gactccgaga
      361 gctttaagtc tatcagcgtt tttctttagc ttttctatat ctgcagtaag cttgttgcgt
      421 tgatgctcat atagttcatt atcagaataa tctcgttccg ggcagtaaca ctggtggaca
      481 acacctttat atgtaaaacc ttctatacca gcatcacccc cttgggtagc aggaattgcg
      541 gtgtagttat ctttttgata gcgaatacga taacatcgaa cgcacaagtc ttcccatgca
      601 ttgccagtca tatatccttc aataaagctt ttgatgctac tcacagcatt ttccccccta
      661 atatttttaa cgaaattgaa tttgtaaagt ctggttcttg agataaggca caaacatcca
      721 cacaaaattc cttcatttcg tccaaatagc gtaccatagc aagtctaaat aatgtgtaca
      781 cctttattac tatcacctct ctatgcatca aaaatagcgt gtcacccatc tttggaggat
      841 tttgaatgct catttcaaaa aactcctttc ctatttgact acctccataa cgacgaatcg
      901 ttgattatca tcgtatacaa acgtaaccta ttctttaaaa atctccatta atgtcttcaa
      961 tattgcactc tagtcttagc ctttttctta gtcatttgtt tattcatggt gctcaacctc
     1021 acaccttgat atatctcgtt gtccggcctg atccgattcg ttttaccgaa ccggtcttga
     1081 ccatggcacc aaggacggct tcgacggttg tggggctgac atcggggaga atcttgcaga
     1141 tctcggcttt ggaaagcggg gtcaggctgt tcagcacggt cgcttccaca cgagcctttt
     1201 tcgtgatctt cttgccatgc accaccacaa atcgcttgtc cagctcctta tagcacatat
     1261 agagggtgga caggaaattc tcgataaagg ggaaatagga attttcgttt gtgtcccacc
     1321 cggcagagga ctgccgcaac gcctcataat agtacgcctt atagttgttg atctgctcct
     1381 caaatgacac atattttccg gcgtcaaagc cgtttttgta cagcaggagc agcgacagca
     1441 ggcgggacat tctgccgttg ccgtcccgga acgggtgaat gcagaggaag tccagtatca
     1501 cgcaggggat caaaagaagc tgattgatat ttgcatcgct tcgggcatcc agataggcaa
     1561 gctccaactg ctccattgcc tttggggttt cgcttgcagg tgtgggacgg aaacggacac
     1621 ggcggtttcc gtctgcgtca acttccaaaa tcacattgtc gtccgtcttg tactgcccgc
     1681 cgtactcata gcccgcaaag ctcatcatca tttcatgcag ccgcagaatg tcacgctgcc
     1741 ggaagtcaat atgttcatat cccaagtgga tctcattcag tgcatctctg tacccggcga
     1801 tttccgcctc gttgtggttc agcggggcgc tgttctgatt gacaatggcg gcgatacgct
     1861 cgtcgctggt tacaatgccc tcaatggcgt tggagctttt aatggactgc acctttgcga
     1921 cagcttccag ctccgtaaaa atctgcgtgt attcgtcctt gcgcacgcct gccatggttt
     1981 tgagcgctgc aatattggaa gtgaggttca caaggtttgc aggcagcaaa ccgttattca
     2041 aaaaggaata atcaaatttt ctcatactgc accaaccttt ctgcacataa ttatagcgcg
     2101 ctatgtgcag aaacgcaagc aatcatctga gttatatgca tattcttttc taataatatg
     2161 tgcagattag gctttgaaat atgcagcata ataaaaaacg gcacggagtt tccgtgccgt
     2221 ttgcgttagt tcgctttgat ttttgaaata ggccgcagca tcaatacttc cgcagaagta
     2281 aatgtgctgc nnnnnnnnnn ttctcatact gcaccaacct ttctgtacat aattatagca
     2341 tattatgtgc agaaaaacaa ggcgccattc gttttatctg catataaatt cttggattat
     2401 atgcagatat ccgctttgca taggcagtaa taagagaaaa cggcacgggg tttccgtgcc
     2461 gtctgcgtca gatcgctttg atttctttga ggtacgccac ggcatcggta tgtccgcgga
     2521 agtagatgtg ctgccgctcc atgtcctcca tttggcgggc aaggttcaga tagtccacat
     2581 acgcccgatg ctcatctgcg gtcacgcgga tctcgccgct gtcctcgttc attttgtcaa
     2641 tgacggggta ctgcgttttc agcttatcca tgcggtcgga aagcgcggcg tagtcctcgt
     2701 cggtattcag cagatccatc acaatatcac tgtcgatctc cgcaaaggcg tcctcgatgc
     2761 gggatggcag gtcaaaataa ttgtcgctca cggtgctcac ttccttatcg tttttattat
     2821 actataccca aaccgcaatg caatatcaaa ggtgcaaatt ttatcgttcg gcctgccgct
     2881 gcattgcgga acgaacagga acggcaggtg aactgatccg atattcttcc gggatgttct
     2941 caatgtcgcc gtcgtagatg ttcacgaagc ggccgccgtt tgaccgcaca tagccgttgt
     3001 caccgaaaat gccgtcttcc tccaagcgaa catcccgccc aaagtcacaa catcgggata
     3061 gggtgcaaaa agtttttcat agtgtgcgcg tgttttcata gcagtacctc tttttcggaa
     3121 aatgtgttgt caatggcaga ggaatacgta taatattgtt tgtgatttat gaatctattg
     3181 cgtgtgttgt tgtgctccgt tttggggtta catccctccg atgtccctcc aaaattcaaa
     3241 attcccgtca aaattggaaa gtgcggaaat gtcagacgaa acggagcttt ttcacacatc
     3301 agtatcaaaa cagccagtaa ttgcgagctt tttggtgtcc gacaacgacc tccacaggat
     3361 ttcaaattcg ctcatattcc gtatgaagca aataactcaa aatccgttgg tagcaatacc
     3421 gtgcgggttc gacccccgcc atcggcacca aactaaagct cgcagacctt tgatatcaaa
     3481 ggtctgcgag ctttagtttg gtatgtataa aatcgttaat aataaatttt atcggttgtt
     3541 taagttaata aaattataag caaatatttt tggcgttcgg acagttgttg catttacttt
     3601 tttcaggaca actatcacat ggaatccaat tcagtttgtc tgaaaacaaa ctacggttaa
     3661 aagtaaactg tcggtgaact tcttgcttta ttcttttaga taaatcacct gaaaaccgcg
     3721 gaccagccac aatttccatg gaatctataa aattgtccgg caattcaatt gccaatcctc
     3781 cacaatttat acttttattt actgtagcaa gcaccctgac ttcacgctca tattcccatg
     3841 ctatatcttt aatatagccc accaaatcgg gtaaatgaga cgtatttttt aataacttgt
     3901 tctccgcttt cccgcatacc aagatttcct cggatttaca ttgtgcggta tcggcattag
     3961 tatatgcaac acgcactatg gataatcttt catagggaat agctgatttt ccaaataccg
     4021 gctttttatt ttcaggataa aacaccggat aaattgtttt agtatttttt atccaattta
     4081 tgaatatctc aacaggtatg cgtataaaca cgccatcttc ccatggttga gcatacatcg
     4141 accacatagc gatactttcg cttgaatcgt acataaaact cgcaaagaac accgcgttgc
     4201 attgttgaga ttctgaaatg tttttaaatt cgagtccatc gttcatggtc tcgggggatt
     4261 ttagccacca agatctgctt ttaattattg atacgacatt ggataacttt gtatagtgat
     4321 atacatattg agcaccattt gacttttgct ttgttaaccg ctttttactg ttgctaagat
     4381 aactaatcaa atcctgcggt gagtctattt tactgaaatt tgttgctgat gtttttttgt
     4441 tcattttgta ttacctcttc ccaattcata gaaattcaat attcttcact caacaaaaaa
     4501 tccgccagat gaacaattct cacaccgttt atgttacctc ccgccaattc atcaagcgtt
     4561 accacatatt tcgggtaatg gtcattgatc tcaagcagat tggcaacctc tctgtccgat
     4621 tcttcgggga gattgcggca cacctgcaca tatatacgct catcgcgccg tacagccacg
     4681 aagtcaattt cctttgcggc gtttttgccg atgtacacat tgtagccttt ccgcctgagc
     4741 tcaaaataca cgatattttc aagcatcgcg gcaacggatt tcggattaaa gcccataatg
     4801 cagtatttca atgaggcatc ggcaagataa aatttttcct gcgttttcaa aatgctcttc
     4861 ccctgcatat catacctttg acaacggtag attacaaaag ctttttcaag ccacccaaga
     4921 tagttataca ccgactccac cgacaacgaa cgaccctcgc ccttgataaa ttttacgata
     4981 gaatttgcgg aaaaggtttt tccgacattt tcaacgatat attttaccac acggttaaac
     5041 aaatcaaagt ttgtaatgtt atgtcttctc gnnnnnnnnn ntcgtgatgt caccggtaat
     5101 gacggaattg taaatacctt ctacaatctg atatgatgaa cgctcatcaa aatttccgag
     5161 cgctacaata gggaaaccac ccatgcgtat atattcgttc aaaagctctt ttgtagtgcg
     5221 acattccttt ttcctgaact ctatatattc agcaaaagac aaagtaaata ccgggatcga
     5281 gatatagcgc cctgtcagat aggtcgatat ttcgcccgac atcagcttgg aatttgaacc
     5341 tgtcacatag atatcggtat cggtatcttc aagcagactg ttgaccgctc tttcccagcc
     5401 tgcgacctcc tgtacctcgt caagtaaaag ataataccgc ttgtcgtcag ttatcttatc
     5461 ttttatatca cggtacattt gctttgccgt catgtcattg tccagttctt ccgatgtata
     5521 gcgcaaataa atcacatggt cggcagaaat accgctaccg atcagctcct ctttcagcat
     5581 atcaagaatg gtcgattttc cgcaacggcg gataccggaa agtatcttta caagcggaac
     5641 atcacgatag gttttcagta tgttcaaata atgtggtctt acgatcataa caccgcctcc
     5701 ttttatttag tataacccga aactctataa aattcaatag tttttaccga aagtcagtaa
     5761 aaacttttaa aatcacacga ctttttcggt tcatataaat gaaatccgta cttnnnnnnn
     5821 nnnacccaca acttctctcg ggttcaagca tcaatttcca caattttcgc tatatctgtt
     5881 gacaaattac gacaaaatat tgcaaaatcc tcccgatttt gttatactat atcaaaaggg
     5941 cggtttaccg ctttgaaatg aggaaacaga tatgagcaca aaatacatat tatacaaccc
     6001 ccttgctggt aacggaaaag gtgaagagca ggcaaaaaag cttacggaaa catacaaaaa
     6061 tgacgagctg aaatattgta atatgacaca tataacaagc tatggcgcat tctttgacag
     6121 catttccccc aaaagcgcta ttataatctg cggcggcgac ggcacgctta accgctttat
     6181 aaatgacagc gagggctatc cgtatgaaaa cccgctttat ttctatgcga ccggaagcgg
     6241 taatgatttt atgcgcgacc tcggcaaaga gcccggctgc aagccgatac cgataaacga
     6301 atatgtcaag ggtctgccta ccgtaaccgt taacggtaaa agcactttat tcataaacgc
     6361 gatcggctac ggaatagacg gttactgctg tgaggtcggc gatgaactgc gaaaaaaatc
     6421 ggacaagccg ataaattata cttctatagc aatcaaaggg ctgctgtttc atttcaagcc
     6481 tgcaaatgcg gtaatcactg ttgacggcaa ggaatacagc ttcaaaaaag tgtggcttgc
     6541 cccgacaatg cacggcaggt tctacggcgg cggtatgatg accgcacccg cgcagacgag
     6601 gaacagtcct gacggcactt tatcggtcgt agtaatgcat ggaagcggta aactcaaaac
     6661 ccttatggtt ttcccatcga tcttcaaggg ggaacacata agtcatacag aaatggtaga
     6721 ggttattaca ggcaaggata tcaccgtacg ctttgaccgt cctaccgcgt tgcaaataga
     6781 cggtgaaacg ataaagaatg ttacggaata ctcggtaaag gcttacgata aagccgttat
     6841 tgcaaatcag ggtaatattt ctataaccga aaaagaaccc gaaacagtgt aaaagccgga
     6901 atacaacaca gtcccgcaga taaatcaaac ctgcgggact tactttttat tcgattttgc
     6961 tcttaagcgc ggaaagatag gttattgcac tgtgcagtgc ggtattgccc tcacctatgg
     7021 cttttgcgat ctgatacggc tttcctgtac agtcgccgca ggcgaaacag ccttttatgt
     7081 tggtcgacat atcacgatct accgcaatac tgccgttgct catttcaaga ccgccgagca
     7141 acacatctgc ggaaaccgac tgctttaaga agaatacgcc gtccacggca atatgcgcgc
     7201 cgtctttgag ggttattccg tctgcatggc gctcaccgtc aacacttacc gcagggtttg
     7261 tcagatgttc gacattttcc tttgaataat cggtcttgta cgatgggtaa aagtacacct
     7321 tccttgcaag ccccgccaga aaatcgacct cttcctcgct ctccttgtta tcgctgataa
     7381 ccgctatcgt tttatccttg tagaaattac cgtcgcaggt ggcacagtag ctcacacccc
     7441 tgcctatgag ctcccgctct cctttgatct cctttaccgt ctgcgcgcct gttgctaaga
     7501 tcacagcgtc cgccttgtat tccttttggt cggcaagcag cataaagtac tttcccatat
     7561 tatatacgcc cgttatacgc tcggctgtta tcacgatatc catctgcttt aaatgagcag
     7621 caagcctttc gttcagctcc ttgccgctga tctcgggcaa ggcgggatag tttgaaatga
     7681 gatgagagca ctctaccttt gcactgaggt tttcgcttcc gaaaaggata aatttcttct
     7741 ttcgtatcgc cgcgtttatc gccgctgata ttcctgcagg tcctgtaccg attatcgcta
     7801 tatcatatga attggtttcg tacatcataa gctccttatc cgaatgtgcc tattgcttag
     7861 ttttatcctg atgctttaca gtataccaca acaggtaaaa atcttcaata ttgagcttcg
     7921 cctgcgcccc gatatttttt tgcataaaaa aacacacccc gaaagcatag ccttcggggt
     7981 gcgtatcaat tcatatgtga taaggatcaa agctcgataa cgatatcatt ttcagccttt
     8041 aagatgctgt cgagaatgag cttgccctca atcttttcac cgtttacagt aacactctta
     8101 acgccgtgtt cactgccgtt cgggttcttt acggtaacat tcagcttctt gcctctgaat
     8161 gtcttcttca ttgtgtattc cttccactct gaggggatag aaggatcaat tacgataccc
     8221 tttgtttcgg ggttaagacc gaggataccc tctgtagtac ctaccataac ggtagnnnnn
     8281 nnnnntagaa gctgttcctg tcagccagtg tacatgagca cgaccggcga acgggctgtc
     8341 cttaccttca acgaactgac cgtaaacata gggctcaaga actctgtgct ctgcgttctc
     8401 gttgtaggtt gcgggagcgg cttccttcca gtacttatac gcgcggttgc cgtgaccgag
     8461 ctttgactct gcgagtatca gccaaccctg aggctgtgag aagatacctg cattttcctt
     8521 tgtgcacttg ttgaagcact gcataagagc gccgtcaaag ccgtgcttga cataaggagg
     8581 atacataacc atagcgccgt aaggagtgtt aagctctctt tctacgctgt ccatagcctt
     8641 ttcagcctgt tccttgcttg caaagccgga aatgactgac catgactggg gattgagcca
     8701 cattgaagcc tcgggatctg ttctctgacc gataacagtg ccgtcttcct tatagcctct
     8761 gatccatctg tcctcgttcc agcaagctga aagtatctca tcgagcttag cctttacttc
     8821 atcaaggtac tttgcgtact ctgcatcgtt cttttcaaga gcgtatgtct ttaagatctt
     8881 tnnnnnnnnn ntactgcgta aacgagctgg aatgctacga atgtagattc gcccttcttg
     8941 ccaagacgta agcagtcgtt ccagtcagcg tgaagtcctg cgggcatacc gtggttgccg
     9001 aggtggttca tcgagaagtc gatagctctc tttaagtgat cgtatactgt gcctgactcg
     9061 ccgtcgttag catatgagat aacctcatct aagaatgcgt ggtcgccgct ttcggagatg
     9121 tacttgtaaa ctgtcgggaa cagccacaga gcgtcatctg cacggtatga ggggtgacct
     9181 gtctgctttg cgtatacgct gttttcgtca ttttcgtcgg ggtggttttn nnnnnnnnna
     9241 tcgtacttaa cgaggggcag acctgcgccg tttgtaacct gagccgagag catgaatacg
     9301 atctgcttct tagccatttc gggagcaagg tggataacgc cctggatatc ctgtacagta
     9361 tcacggtagc cgaagccgtt tctcagaccg cagtactgga acgatgctgc tcttgaccag
     9421 atgaatgtga tgaagcagtt atatgcgttc catacattta ccatgttgtt gaagtcggca
     9481 tcgggagtat ttacctgcag attgtcgagc tttgagtgcc agtagttctt aagctctgtg
     9541 aggtcggcct ctacagcgcc atccttttca tacttagcga tgatagcctc aatttcggcc
     9601 tctgtcttct gaccgccgag aacataagtg agaacctttg tttcgccggg ctgtaaaacg
     9661 atgtcgctct ggagtgcgcc gcaggaattt gtattgtagt taaggtcgcc ctttaagcct
     9721 tcttcaatgc ccttggggtt gctgtaggtt ctgtaagagc ctacaaatgc gtcacggtcg
     9781 ccgcagtaag catcaacctt agctgaagca agacctaaga aacgtccgct gttggggttg
     9841 tatatctcat tctgcatctg gtggatatac ttgttcttga agtatgttct tgtgatgaac
     9901 agggtgtact ggaggtttac ctgatcctgc tcatagttgg gatcgtttac gaactcgcag
     9961 tagcctgtaa cgctcagctt acggggcttt gtatctgtgt ttgttacctt tgcagcccat
    10021 acttcataag tagcgtccat gggaacatag taagtaacct cagacttgat cttcttgtac
    10081 tcagatgtga tgacagtgta ggctgtaccg tgacggcact cgctcttgta ctggtctaag
    10141 ggcttgcata caggtgccca tgatgccgac cagaaatcgc cgtcctcagc gtccttgata
    10201 tatacatatc taccgggctg gtcgttggga acagcattga aacggtaacg gataacacgt
    10261 ccgtttgcac cggatttaac gaagctgtag ccgcctgcgt tgtttgaaat gatagcaccg
    10321 tattcgggtg agcctaagta gtttgcccat gccgagggag tatcgggtct tgtgatgaca
    10381 tattccttgt tttcaaggtc aaaatgtccg taattcatca gaaagtcctc cttgatatta
    10441 tattccccgt aaactagctg ttttacgggt tttaaattcc tttgcgtaac ttcattatat
    10501 cagtaaatcg ttttcatttc aagggcggta ttgtgcacaa atttagctgt ctgtttttgt
    10561 gcagtattgc catagtgtca aatcaacgcc cgtaacggat ttttggcggg caaaactcgg
    10621 aaatttccgc ataaaacccc attttacagt attttaacat tgattttacc gattttaagt
    10681 agtataatta ttcaaagaaa aataaaaaga acaaaagaca agaggtaaac ttatgaactg
    10741 ggcaattatc gagcaggtcg ttataatggc tctgctcgca ggtgtcggca tattatgtcg
    10801 aaagcttaaa ctgcttgacg atgcctgcac aaaaaaactt tcaaccgttg tacttaactt
    10861 tgcaacgcca atggttattc tggtatccta ccagaagccg ttcgaccccg aaacgctgaa
    10921 cacactgctt ttatcgttcc tcatagcgat aataagctat gtcatagcgt ttatccttgt
    10981 tccgctgata gtacgcagta aggacaggga gggacagctg atagagcgtt tcagctgtct
    11041 ttattcaaac tgtgccttta tgggcatacc gctgatacag ggcgtttacg gcacagaggg
    11101 tgtgttctgc cttacggcgt atgttacgat gttcaatctc tgctcatgga cgcacggagt
    11161 tgttatgatg aagggcggaa agcctaactt taagcagatg ataaagtcgc tgttatctcc
    11221 gagcatcatc gttacagtaa tcggtattat cctgtttttg tgcaacttca cgctcccgaa
    11281 cgttatcctt gaaacggcac agcaccttgc aaatatcaac acaccgctcg caatgataat
    11341 agcaggctca actatagcta cggtaaagct aatccctgca ctgaaaaagc caagacttta
    11401 ctatacctgc tttgtaaagc tgttcctgct tcctgcactg cttaccgtta tctgccttgt
    11461 atttaagata gatcagaccg ttgcgggcgt aaacatactc gctaccgcct gccctgccgc
    11521 tgctatgtgt acaatgtttg ccctgctcta tgacagggac gcgggctaca gcgccgaaat
    11581 atttgcggta agcaccatat tatctatggt gacacttccg cttaccctta tgtactacac
    11641 cgcacttgcg ggtattttca tgtaatacag atacataaaa acaatatata aaggagcatt
    11701 tctcatgaca gacagcatga taaaaaattg cgccgcatta ctcgatatac tgagggagaa
    11761 gaaccctctt gtacactgca taactaacta tgttaccgca aacaccgttg caaacggact
    11821 gcttgcggcg ggagcatctc ccgttatggc ggacagtccg cttgacgctg aggatataac
    11881 cgcaaaggcg tcggctgtgc ttttcaatat gggcacactc agcaaggaca gactccgcgc
    11941 tatgctgatt tccgcacaga aggctgcgga cacggcaaag cctatcgtgc ttgaccctgt
    12001 aggtgcaggc tcaaccgaca acagactgcg cagcacaatg cagctgctcc gtcagttcag
    12061 atttgccgca ataagaggta acgcgtccga aatatccgca ctgctttcca caagccccat
    12121 caccatgaaa gccgaaaaag gcgtagacag cgccgaaaca acgcttgagg ataagatcgc
    12181 acttgccaaa aaggcggcaa agcgtttcag ctgtaccgtt atggtgacgg gcagtaccga
    12241 tgtcgttgca ggcggcgata aggttgctct tgtatcaaac ggaataccta tgatgagcaa
    12301 gatcaccggt acaggctgtc ttgcttcggg gcttgtcgcg gcgctgtgcg gctgtaccgg
    12361 ggacagcttt gatgcggcgg taacggcggc ggcgcttatc ggcacagtag gcgagatagc
    12421 aggcgaaacc gcaaagggta caggctcgct gtatgtcggt atgcttgaca cgctgacgaa
    12481 tattactccc gatattttca cacaatacgc aaagatagaa aattacacag gagaataata
    12541 agatggaaga tctgaatgaa atccgtgagc ttttcgcaaa agaccgtttc gctacggaaa
    12601 acggcgcagt gatcgaagcg gtaggcaaaa acacggctac tgtcggtatg aagatagaac
    12661 cacgccatcg caatgcggca ggaacggtta tgggcggcgc gattttcacc cttgccgatt
    12721 ttgcctttgc ggtagcgtca aaccacgaga aaataggcac tgtatcgcta aacgcaaata
    12781 taacgttttt aagagcctcc aagggcgata agctcatagc aacggctgaa tgcgtccgtg
    12841 acggaagaac gacctgttat tatcgtgttt cggtcacgga taacaccggt gtgcttatag
    12901 ctgagattac aacaagcgga tataagacaa actgacttga gcaagaacgc agggcagttt
    12961 tcaaagtata atacttttta gcggaatatg cttaactcct cttaaatcct gattgccgtc
    13021 agtcataggt tttatgccgt ttcccgcctt ttttcgaccg ttatacgcca cactgttgac
    13081 aaaaaagcgt gagtatggta aaatgtgttt gtattacagt gtaaatacac actgttaata
    13141 tggttatatt ttaggctgat tgaataacag gaggatttta ttatgaacaa aactgcggca
    13201 atacttattg catttttcct tggcggactt ggcgttcatc gctttatggc aggaaaaata
    13261 ggaacaggca ttatctggct tcttacaggc ggttgttgcg gtataggctg gcttgtagac
    13321 ctgattcagg tttgcacagg caagtttact accaaagacg gcgcaccttg ggtaacagag
    13381 cagtaattta aaagaataac gccccggctt ccatttgaaa accggggcgt ttttgtccct
    13441 tttagctctg taacaaatgt cgaggaagcc aagagccaat acaaaatccg cccccgtttt
    13501 cacgggggcg aactcatcga tatttcttta agcggagttt ggggcgcagc cccaatgtgg
    13561 gttgtcagtc gttggtgtcg ctgcgtctgt gcattgacag ccatttgtgc cgttgcatct
    13621 gtgcattgac agccatttgt gccgttgcat ctgtgcatcg acagccattt gtgccgttgc
    13681 atcgtgcatc ggctttatgt ggctgtcttt atgcatcctt gcatagcgac tgtgtgcgac
    13741 tgactacaag cgtccttgct ttgtgagggc gacggcccca cttcccacaa aaataaaatc
    13801 cgcccccgtt ttcacggggg cgaactcatc aagtcattaa tttataaata ttaagccctg
    13861 aaactcacgc tgttctcaga ttactggagt aactggagaa tagcctgagg ctgctggtta
    13921 gcctgagcga gcatagcctg agatgcctgc tgaagaatgt tattcttagt gtagttgatc
    13981 atctctgtag ccatatctgt atctctgatc tgagattcag ctgctgtcag gttctcgttt
    14041 gttgttgtca ggttgttaac tgtgtactca agtctgttct gaagtgcacc aagacctgct
    14101 ctctgcatag atactgtctt aacagcagca tcgatcatat cgatagcagc atttgcagcg
    14161 tcctgagtag aaacgtcagc attagcaaca cctatagcag aagatgacat atcgttaacg
    14221 ttcagagtaa ctctctgatc ctcaacgccg ttagcaccga tctggaatgt aagaccctta
    14281 ccgttgttag gaactgaatc cttgccaaca ccttcaccgt ctgtgatagc tacttctgca
    14341 ttacccttaa cgttgaagta aatcttaccg tctgcatcgc catcagtatt cttggtatct
    14401 tccagcttta ctgagtagtc atatccagct tccttaagga tgttttcaat atccttttct
    14461 gtatatgtct tacctgttga aaggtggagc tttatttcag cagccttagc agggttacca
    14521 gcggttgcag cggtagcagg cgcttcaaca gatacatttt ctttacctgc tgcaacgtct
    14581 gtagatatag taatcttaga agccatacct gtatcatatg ccttgccgta tgagttagcg
    14641 gtaaatgtga tgctgtcaga tgaaccgtga gcaccgctct ttgtatcacc aacaagcttg
    14701 agcacatcgg cactatttga acctgtagca cgaagacctg cttttgtagc gtctgtttcg
    14761 cctgccttgg aagctgtcat aacaccgttt gcaagcttaa cagaaacttc tgcaggagcc
    14821 gttgtctgac cggcatcctt agactgtgta gcgttagcga tcaggttatc gatatccttc
    14881 tgagtgtaag tttcaccgga aacaaggttc atagtaagat tcttaccatc agctgaccaa
    14941 atggcatttt cgccgccctt acctgatgct gcataagctg ttgtaatttt aactcctgct
    15001 atattagatg tgattgtagc accgataaga tcagtatctg ctgttgaaac ggcaccgtac
    15061 ttagcaccgt attcgtttgt accctttgag ccgccgagtg aaccatcgag gagcttcatg
    15121 ccgttgtact ctgtagaaga agcgattctg tcgatttcgc tcttgagtgc gtcaacttcg
    15181 agctggatag cttcacggtc tgtatcatcc tggtatgtac cgttagctga ctgatctgaa
    15241 agttctctca ttctctgaag aattgagtga gtttcgttta agccgccttc agctgtctgg
    15301 ataagagaga taccgtcgtt ggcattcttt gtagcctgtg aaagaccacg gatctgtgaa
    15361 cgcatctttt cagatatagc cagacctgca gcgttatcgc ctgcacggtt gatctgataa
    15421 cctgaagata acttttcagt tgccttcttt gtacctgaaa cgttgatgtt catcttgttg
    15481 ttagcattga tcgcatttaa gttatgctgt actaccatac ctgccatttt gttttcctcc
    15541 ttgaatgtac tcccgaaatc cttttcggaa gtcgttgccc ttatagggac ttgtttttgt
    15601 tagtgtgacc ttgctttgcg ctaagcttcg gtcgattggt gtttgttttg caaaacactg
    15661 cggaactgcg gaatttatct ctgcctccgc acagggctct gtgaccctta catttattat
    15721 atcggcggta atttttctaa actttagcct ttttgaaaac ttttttataa tttttctgag
    15781 aaattttata tgcggtttgt gtaaaattta caatatgaac tgttcggaag aagatacagc
    15841 aaaaccgccc tgcgtcacag agcggtttca gattgtagaa aaagtctgtt tttttagaaa
    15901 tgtagcttat tctcaaaccc taacccc
//
LOCUS       DAWAOG010000004        15764 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_729_length_15764_cov_2.657585, whole genome shotgun sequence.
ACCESSION   DAWAOG010000004 DAWAOG010000000
VERSION     DAWAOG010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 15764)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15764)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15764
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_729_length_15764_cov_2.657585"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3425..3434
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4298..4307
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5798..5807
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4068 a         3670 c         3606 g         4390 t
ORIGIN      
        1 ggtgcggact tactgccgtg tctgcgttcc atacgataca caattacatt gataccaagg
       61 agcgcatact ccgcaagggc gcaatttccg caaagagcgg cgagcttgtg cttattccga
      121 taaatatgag ggacggaagc gtgcttgcga gaggaaaggg caatcccgac tggaactgtt
      181 ccgcaccgca cggcgcaggc aggattatgt cgagaacaaa ggctaaggaa acgctcgaca
      241 ttgagcagta taagaagagc atggagggca tatacacgac ctcggtcaat aattcaacgc
      301 ttgacgaagc gcctatggcg tataagtcac tgccggatat tattgatgtt atcagagagt
      361 cggtagatgt tattgaagtg ctgaaaccga tatataattt caaggcgtgt gagtaacctg
      421 ctctgcgccg cagaggagga aattacaatg cttgaatact taagagaaga ggcaaatatt
      481 acgctgaccg aaaacggagc aacgacctac ataagcacaa aatcgcactg ccttgatctg
      541 ttcgcttcaa taggcgctat ccgtaagtcc cccgaaaacg atataatcga ccgctttgta
      601 aaagcgttgg cagagaacag agatcttgcg ctgaaaacgc ttttctttgc gcgtgatgcc
      661 cgtgagggtc tgggcgaacg cagagtgttc cgtataatac tgaactatct tgcgaaatat
      721 gagcctgaga gcgtacgccg aaacatagaa tacatcgctg agtacggcag atatgacgat
      781 ctgctgtgcc ttatcggtac gccctgcgaa aaggacgctt tgcggattat agaaggtcag
      841 ctcaaaaagg atatagcgtc tgacacgggc gtgtcgcttc ttgcaaaatg gctcccttcc
      901 gtcaatgcat caaacaagga aacggtacgc acggctcgcc gtctggcgcg tctgctcgga
      961 atgagcgaaa tacagtaccg taaaacagtt gtcgcactca gaaaaaagat cgatatagtc
     1021 gaaaaccgcc tgcgtgtgca ggactatacc ttcgattact ccaaaatacc cgctcttgca
     1081 atgctgaaat accgtggtgc gttctatgag aacgactttg accgttactg tgagtatatc
     1141 gacaatgtaa aaaacggcaa agcaaaaatg cacacaggag tactgacacc atatgacgtt
     1201 atcgctccgt gtttcaattg gcgtttgaaa gaactttccg ccgaggagcg cagcgcaatg
     1261 gacgtgacat ggaacgccct tgaagatttc ggcaacgatg aaaacgcact tgccgtgatt
     1321 gacggctcgg gttcgatgta tccgagagcg atagctgttg cactgtcgct cgggatatac
     1381 tttgccgaaa ggaacaaggg taagttccgc aatcatttca tcacattctc aaaaagacct
     1441 cagcttgtcg aaataaaggg cagggatatt gtcgaaaagg taagatactg ctgtcagtat
     1501 ggcgaggtag cgaacacgaa tatatcggct gtgttcaagc tgttgctcaa aaccgccgta
     1561 aagtatgacc tcccgcagga agagctcccg aagaggctat atataatttc ggatatggaa
     1621 tttgacgagt gtgcggaaaa tgcaggtgga acaaacttcg agtgtgcaaa gagaaagtat
     1681 gccgaaaaag gatatacgct ccccgagata gtgttctgga acgtagagag cagaaataat
     1741 cagcagcctg tcacaatgaa cgagaacggt gtagcgctgg tttcgggctg ttcgccccgc
     1801 cttttttcga tgatggcaag cggtgaactt gacccgtata aagctatgct tgatgtgctt
     1861 atgtctgagc gttacgccaa aatatcagca taaatgaaac cgctgtcagg tgacagcggt
     1921 tttgtcatat aaacagcaac ggtatcggtt tgaccgatac cgttgtctga aggaaaagaa
     1981 ctccgccgca ataagagcgg cgggaagaaa aacaactgta aggagttgtt ggaattattc
     2041 gcttactgct gtagatgagc ctacttccat agtacccttc ttcataacgt attcgtaatc
     2101 gttaccgtca gagcccttaa ccttgaatgt gagcttgtca ttttcgtaag taacgctcat
     2161 atcgatctcg tccttgctgc ctaccttcat tacttctgca ccgttgctct tgtactgaac
     2221 attgcgctct tctgtagctg cggctgactt gattataacc ttgtcatctg tgaatgtcca
     2281 tacagatgcg aaagaagact cgtcaacacc gagctttttt gcatagtctg caacagtttc
     2341 gccggcgatc ttttcgtaga tccagtcacc cttcatcttg tcctttgccg agcctgagca
     2401 agctgcgagc gccattacca ttactgctgc cattgcaagt gcaatgaccg acttgattac
     2461 ttttttcata attattcctt tctttgcggt atgtccgcct ctgattatgt agtatcggtt
     2521 gtttttatca accttagcaa attatactca ccgaaatggt ttaattcaaa catctggcgt
     2581 taatcggtta aaaatgggct ttaatcggtc ttaaccgccg aagaaacttg taatactgaa
     2641 accggtcggc ataggcagtg taaacatata taccgtagca ataacaaatg caaccagcgc
     2701 tataataacc accgcatatt tgtttgcctt gtggttgaaa gaacctgcaa taccgagtaa
     2761 gaacagtaca acgctgtaga taaccgttac aagaccgaat gcgtcgccgt ttgaattatc
     2821 cttctgaccc tgttcaagta catcctgaga ctgtgcaaga agctcgtttg cttttgtgaa
     2881 atagctgttg acgtaattct cgtcaaagaa gggcgagttc atactgtttt ccttttcgag
     2941 ccacgcaagt atagttgcct gaggatcgct gctgctgttg tcagccgtgt tccagtcaat
     3001 ctttgccgcc attgtttcac tcacgttctc cgccatcttg tagaacagct ggttgcacag
     3061 ctttgcaagc tcagcctcgt tattattgtt ctgtgcaaag acgatttcaa tctgcaatga
     3121 gcttatatcg ttccagagta tcatatcctg attcatctgc tgaacgcctg cattatattc
     3181 cgagttgccc tcggaagcaa gattgttgct ggtggcgtag ttggtagcct gatttccgcc
     3241 gtgaagcgag cctatccagc ttgcccacgc tgtgagcagg gcggtaacgc ccagcattat
     3301 tactattata agctcggtga gcttgtccat atccggcttt ttctttgcat agctgtccgc
     3361 attgtttttg ttttccatag aaacctccgt atgatcgtat tattattttt attatatcaa
     3421 tcgtnnnnnn nnnntcggcg gtttttcggc agtaaaagac agttaaccat atgaaaatat
     3481 tgcaacatcc aagaaaatgt agtataattg tggtaagagc ataaaaaacg gaagatgccg
     3541 cttcaggcgg aaggtacata taataatgga actgagaata gcaataaccg atgacctcaa
     3601 ttcggagcgt gaaaagctgt catcggcaat agcagctccg tttgaaaaag tggggctggc
     3661 tatcgggaat atagacgaat ttacaagcgg cgaggctttg ctcaggcagt tttcggcagg
     3721 aaaatacgat ctgatatttc tagatatata tatgggcgga ataagcggag ttgaaaccgc
     3781 aaagcgtata aggtccatcg ataagaatgt tatgctcgta tttgttacga caagtaacga
     3841 gtttgcaagc gagagctatg cggtaaaggc aaacttcgat acgctcagac agcataaagc
     3901 agcttgccga aacgctggca cagcagatgc agaacacaag gagcgttgcg gcgctccccg
     3961 acggaacggt gataccgctt tcatcgcttg tgtatacatc atgctcgggt cattatgtaa
     4021 atgtatatct tgtcggaagt gaaccgctta agatccgtac aacgcataag ataatgtacg
     4081 aagcgctctc aaaatacgag gggataattt catgcaataa gggcgttctg gtttcgcttg
     4141 ccaatgttgc taagcttgac ggtgaaaatt ttactatgat aaacggcgat accgttccta
     4201 taagcagacg taagatgacg cagatcaaaa acgactatac acgtttcgtt ctgaatcgta
     4261 ttaacagcga atttgagaaa gagcaagggt gatgaatnnn nnnnnnnctt tatcaatgag
     4321 gctctatcct gccttgttct tgtacccgca acggcattgt gccttttccc gatgaaggac
     4381 agaataaaat atccgttgaa acaggtaatg ccgctgttcc tgggcatact tgtatcggtc
     4441 actgcgttag tttcctgcgc ggccgcactg ctccccatag accgcaagac ggtatgctat
     4501 ctgttgctga taccgttgtt tttcacttac agagttataa tagatacaga tatattcaaa
     4561 tgtctcgcat cgtttctgct gtcctttacg ataatgagct tttgtaagaa ttatgccata
     4621 atggtcgatg cggtcataca cccggagctc ggcactccgc agttaagcac tgacggagcg
     4681 ataatacagc ttggcatatc gtgtgcagtc acgtttatac ttgcgtatcc tatgatcaaa
     4741 ttcggctctc atcttataaa cgaattgccg tatacaaggg tgtggcttat atctatagtt
     4801 atgtcgctga tgtttatagg ctttaatttt gtcgtgcagc ctgttcatta tcaaacgctt
     4861 tatctcaacc gtgtatttct tgtatatatc attatcacta cgctgatgtt ccttatgctc
     4921 ctgatgatgt atacggtgtt ctattttatt tcatccgcgc tgttaaaagc gggcagggac
     4981 aaagagcata tgcgtgtgct tgaaatgcag aaaaagcaat atgattctca acagaaatat
     5041 cttgaggata cctcacgcat acgtcacgat ttcaagcatt ctatagttgc tatcaaaaca
     5101 cttgccgagg acggagatta cgaagagctg aaaagctatc tggataatta tattgacacg
     5161 ctcccgagca gagtaccgat acgctactgc ggaaactatg cgctcaacgc gcttttgtgt
     5221 cactttgaag aacgggctaa agataatgat attaagctca aaatcggagt cgatatcaac
     5281 gatagctccg tgccgctgtc agatgacgag ctttgctgca tagtaggcaa cataatagaa
     5341 aatgcagtaa acgcaagtct tatgctcccc gaaaacgagc gcagtatttc tgtttcggta
     5401 aaaaccgatg acgcgtccgg caacctatat gttgtggcgg aaaacaactt caatggcaag
     5461 ctgagatatg acggtacgca ttattattct accactaaag gcggcaacgg tatcgggctt
     5521 gaatcgatcg cgttcacagc ggaaaagtac ggaggaacag caagctttta tcataaggat
     5581 aatgtgttct gctccgatgt ggtcgtgccg ctaaatgtgt aaaagaataa aagtaaaagc
     5641 cgcagagcga tgtcagcatc actctgcggc ttttattttt taccggtctg atatttatgc
     5701 ttttttctca cttgtttgtc tgaacatcat attgataacg cctgtctttg caagcgcaaa
     5761 catcagaaca gatgcgatga gcgcaccgcc taccgtgnnn nnnnnnnctt acgccaaatg
     5821 ggataacgta agtgaaaagt gcggcttctt tacccataac aaatactgcg ataggataag
     5881 ccgccatagc accgaggata cctgtaccta tgatctcgcc taagaaagct atcggaatac
     5941 gctttgcaaa tgacagcttc ggaagaatgt atctgaatgc aagaccgcag cagagagcgc
     6001 ctaccatgct tccggggaat gcgagcagcg tacctgtacc cattataagt cttataagag
     6061 atgtgatgaa tgccataccc acggcatacc acggaccgag cagtgtgcct gctattacat
     6121 ttaccatatg ctgaatggga aaacattttg aagcacctat cgggatcgaa aatccgctta
     6181 acactacgct cagtgcaatg agaacgcctg ctatcgcaag cttctgaatg ctgacttttt
     6241 tattcatact aaaaatacct cttttctttt attcgtcaaa gaagctccca cactgtctgc
     6301 gggagctgat gtttttatgc gcactaaaac aaccgttccc tacgttggca ttacccaaat
     6361 caggtcataa ggtcggaatt gaaaattccc tctcagcccg tccacgagct ccctttattg
     6421 tgtataggat ctgtctgaca gtcacagtat ataacacatt tgcggcggtg tcaatgctgt
     6481 ttttaagata ccattgttat gtatatgtca aattattcca taagtaacat aacgtttgtc
     6541 ggtagcataa aatgtatcgt aaagccgaaa aaacacgaat gaaaggagtt tagctatgga
     6601 aaaagatgat tttttctgcg gcaataggga aagtatatcc gaaaaaaaga aagtcggata
     6661 tgcatatgtg ccgtttcagc ggctcggcaa gctctacgac ccggaaacgg cgatcaggac
     6721 gggaacaata ttccctgagc tcaacattac tatagacgaa tatgaaaggg ggctttacag
     6781 tggcgtataa tctgtcggca agagcgtcgc aactgctcag aaagctgtct gcggcagatt
     6841 tctatgcgca ggatttaaag cttttccttg acactcaccc aaccgataca aaggcgcttg
     6901 agctttaccg cgaggcggtg aaacagaccg aagcctgccg ctgtgcgttt gagaatgagt
     6961 tttatccgct cagagcctct tcggcaggag cggattgctg ctgggattgg ctaaatgggc
     7021 aatggatatt ataaggagga cgtatgttta tatttgaaaa acgaacacag ataccggtcg
     7081 tgataaagaa ccgtaacccg aagcttgcaa gctacattct ttctcagtac ggaggtccgg
     7141 acggtgagct cagcgcggcg ctgagatatc tttcacagcg cttttcacag accgataaga
     7201 gggcaattgc gatactgacc gatataggtg tatcgttgcg ggaatgatcc cctgcgggaa
     7261 tttatatatc aaatgttttg catatgtgaa aagataccgt attttttgcg ggtttacaaa
     7321 ttcaagtcat tgttcataac gtagtgcggt tgactttatg tatcattaca tagcgacctt
     7381 gtgcgaccgg tacaagtatc agtgccttac ctgcgtcagg atttaaacaa gtgtatttca
     7441 tattgcactg tataacggca aaaagcaaaa acgcagagtt gcctctgcgt ttaatattta
     7501 tatataaatc cggtcaatct tcctttgtgt tctgatacgt ctttaatata tcctctgcaa
     7561 cagctctctg tgtctttctc agatccatcg cctgcttttg tatatgccgg tgtgcctgct
     7621 cctcggtaag attaagatac tgcatcagca tcgactttgc acggtttact atcttcatat
     7681 cggataactg ctgtgacagc tgcctgttct cttcttcaag ctttgccatt ccgatatgtg
     7741 ctacttcggc aagctttatt gtttggacaa gcagagcctt gttgaatggt cgcggcagta
     7801 cgaaagctcc tgtaaatcgt atctttttct gcacttcgtc tgcaatttca cgctttgcaa
     7861 gcactactac accgtttttc gtatctttgg caatgtcagc aacaagatca agcccaaatt
     7921 cgtctgataa tggagtggaa attataacgc tgtcgaattt tgatatatca acacctctta
     7981 tgatagtgcc gctggtgaaa atcgagatat tgtcgcagtc aagctcaagc aacagctgag
     8041 ctatgctgtc gcatatttct tcggtcttgc ctgctatcag tatgttcatt gcggcaccct
     8101 ctttcgtggt attaaacgct ccgccgtcac gcctttatat cgacagcgcg ttgtatattt
     8161 atatctattc cgtttatcgt cagttatgcg tgtacagctg tttgtagggg cttgcagtat
     8221 tcggtgaaag cttataaaat ctgcccttta acagcgtatt cgtatcatat ccgaaattct
     8281 tcgcgaactc gtcaacatat tctctgatat cagccttgtc gctgtcgtca gcaacctcgc
     8341 atgaaacctg tacgggaaga cccttgggag cttcgtcact gcggatatac atatgaccgc
     8401 cgtgcattcc cgttccacag aacgagccta caggaacctt gccgtcacag ccgatgccga
     8461 gcaccacgat gatcccgccc gcctggtatt cgccgaggaa atctccgacc ttgccgccga
     8521 tgattatttt cgggtagaac tcgtgatatt ctttcatatg tattcctact cgatagcccg
     8581 catttccctt tacgaatatc ttgccggaac gcattgcata gcccgttgta tcgccgcagt
     8641 tgccgtgtat gaatatctca ccgttgttca ttgtatcgcc gatagcgtcc tgtccgttgc
     8701 cgtgtaccac aatggtgctt ccgtcaaggt atgcgcccag agcatttccg ggagttccct
     8761 ctatggtgat cgtcttgccc gaaacgccgc atcctatgta gcgctgaccc attgcatcgg
     8821 ttatcgttat atccttgtcg gcacattcaa ctatcttttt attaaggtcg taataaccta
     8881 tctcgttagc attaaatact gtcatggctt atcctccgtt attgcagaca ttacaggcat
     8941 catcagtgct ttttgcctgc gagcttttct tcgcgtcttg ccttgccgaa catcatcatc
     9001 tgcggctttt ctctcagaag atcaaggtca acctcggtaa gcggtatctg ctcctttatc
     9061 atcgaggtgt aaatacctgc tcttgcgaca tcgcccatca ggataaagcc cttcagcaga
     9121 ccgtccttga atacgagctt cttgtatatc tccttgtctg cgtcctctgt gatatcctca
     9181 tcgccgtcat agcttccggc tgttatgata tgcagaccgt agaagcctat ggcgttcatc
     9241 ggtatagcgt tcagataaag cgtttcctcg cctgccatat tcttgcctgc gacctcgccc
     9301 tgcataaatg cgttaggaag aagagcaagt atcctgtctg tatttgacga gcaatcgtgg
     9361 cttactgtgc agtcgcccgc ggcataaacg ttgtcgagcg ttgtctgctg gtgaagatcg
     9421 gttattatac ccttttctac cttgccgcct gcgtcgctta caagctctgt gttaggtctt
     9481 acgccgactg caacaacaag aatgtcaaag tcgaccgtgt cgccgtttgt cagctttgca
     9541 ctgttctttg agaactgctc aacgcttgtg ccgagtatga actttgtacc cttatcctcg
     9601 atatgcttct gcattatctc acctgcacgg atatcaagaa tactgggtag tatcctgtcc
     9661 gcaagatcaa ccacagtgat atcggcgttc aggtggtgca gagcctctgc gcacttaaga
     9721 ccgataagtc ccgcgcctat tataagcacc ttagtaccgg ctgttacctt ttcgcggata
     9781 gcctttgcgc tgtcccatgt cataaaggtg aacctgtcct ctacctcgtc aagtcctttc
     9841 atcggaggaa cgaacggttt tgaacccgtt gctaccaata acttatcata ctgtactttc
     9901 tctccgttgt caagtaccac acagccgttt tcgatttttt cggcacggac agagaagatg
     9961 acctttacat tgttcttctc gtagaaatca ggctctctgt agtatatctt gtcgtcggca
    10021 accttgccct caagccagta gcttataagc ggtcttgaat atgtgtgata tgcctcgtca
    10081 gtgattatcg tgatattgcc tgtcttatca accgagcgta taccctgaac tgcaccgata
    10141 gcggcggcgg agtttcctat gataacataa tttttcatct tcgctcctcc ttatctttct
    10201 tcaaggacga tcgccttgtt cggacaaccc tttacgcatg caggctcgcc gttattgttc
    10261 ttcatacaaa gatcgcactt ctgtattacc tttgaatttt cgcttgttac gatacagccg
    10321 taaggacagg caagtacgca ggtgtagcag cctacacaac ggctctcgtc acacgagata
    10381 acgccgtcct tttgtgataa agcgcctgtt atacagctct ttacgcaaag aggagtttcg
    10441 cagtggcggc actgtacggc aaaatttatg ccgctgccct cctctacctt tattctcggg
    10501 ataggcttat tgccgtttgc gaaagccttt atcatgcttt cagctccgct gtttgcggcg
    10561 gcacagtaat attcgcacag atggcacgca aggcaccagt cttcgttaac gtaaactctt
    10621 ttcattttac cttacctctc ctttatcact gacctgcgtg ctgtatgccg agtatctcaa
    10681 gttccttttc gttaagaccg atacctctga gcataagtct gttgcctctg agagcctcga
    10741 ctgagttgat acccatacca cccatcattt ccttgatctc gtgatcccac gcatgaacaa
    10801 ggtttacaag tcgctgatag ccgatgttcg ggttaaggcg ctttacaagg tcgggtcgct
    10861 gtgttgcgat accccagttg cacttgcctg tctgacagct tctgcaaagg tggcaaccga
    10921 gcgccagcag agcggcagta ccgatgtagc aagcgtccgc accgagcgcg atagccttta
    10981 caacgtccga gcttgaacgg atagaacctg caactactac agaaacctcg tttctgatac
    11041 cctcatcacg cagacgctgg tcaacgcttg caagggcaag ctctatcggg atacctacgt
    11101 tatctcttat tcttgtgggg gctgcacccg taccgcctct gtaaccgtct atagcgatta
    11161 tatccgcacc cgaacgtgca ataccggaag cgatagcggc aacgttatgc acggcggcga
    11221 tctttacgat gaccggcttt ttgtactctg tagcctcttt cagtgaaaga acaagctgac
    11281 gtaagtcctc tatcgaatat atatcatggt ggggagcagg tgatattgcg tctgttccca
    11341 tggggatcat tcttgttctt gaaacgtcct cgaggatctt tgctccggga aggtgaccgc
    11401 cgataccggg ctttgcaccc tgacccatct tgatctctat tgccgcgccc gtttcaagat
    11461 aatccttgaa tacaccgaaa cgtcccgatg cgacctgcac tattgtattc ggaccgtact
    11521 gatagaagtc cttgtgcaga ccgccctcgc ccgtgttgta gaatatgcca agctctgtag
    11581 ccgctcttgc aaggctctca tgagcgttac ggctgataga gccgtatgac atagccgaga
    11641 acataatagg cgttgaaagc tcaagtgtag gtgttgtttc cgtctttaca ctgccgtcct
    11701 cgttgaacga aacgtttttc ggcttctttc caaggaagac ctttgtttcc ataggctcac
    11761 gcagagggtc gataggcggg tttgttacct gagaagcgtt aagcagtatc ttatcccagt
    11821 aaacaggata ctccttcggg ttgcccattg cggaaagaag aacgccgcct gtttctgcct
    11881 gcttgtagac ctccgtcatt atctgctggc tccagttgga gttgtttctg aacttgttgg
    11941 ggttatctac tatcttcaat gcacctgtag gacagataca tacacatctc tggcagtcaa
    12001 cgcactgctg gctgtccgag atcatcatat cgagatcgct gtcatatctg tgtacctcgt
    12061 ttgcgcactg tcttgcacac gcctgacatt ttatgcaacg gtccttatct cttataacat
    12121 caaataaagg ctgaaaataa ttaagtgcca ctgttaccgc tccctttctt acttcttctc
    12181 gttcagctta acgatgatag gctcgccgcc tctgggtgac cagatacggt caacaacggg
    12241 gcatatagct cttatcgcac attcctcact tgcaaggtaa accatatcgc ccttttctgc
    12301 ggctaccatt gaacggagct taagtctgtc gttgagtgcc ataagaccgc cgtcaaagcc
    12361 gagaattatc gagaacggac ctgttatcag aaggcttgag aatacatttc tgaaatattt
    12421 cagcttctgt ctttcgctct cactgtactt atccgagtct atctcgctcc agaacggagc
    12481 ggcaattatc tttgctatat cttcaagaca cagcttctgc tttctgttga ggaagtctat
    12541 tatatatgtt atgacctctg tatctgtcag caggttgcac ttgtagccgt acatttcgat
    12601 gtatcttctg tttgcatcgt aggaagatat ctcaccgtta tgtacaatgg tataatccag
    12661 cagtgcgaac ggatgagcgc cgccccacca gccgggtgtg ttggtaggat atctgccgtg
    12721 cgccgtccat gcatagcctg cgtactcttc aagtctgtag aactctccga catcttccgg
    12781 gtatccgaca gccttgaata cgcccatgtt cttgccgctt gagaaaacgt aagaaccgtt
    12841 tatcttgtcg tttatgttga taacgcactg tgcaacgaac tctctctcgt caagctggct
    12901 ttctgtgagc tttgtcggca ggggatttac gaaatatctc cagatgagcg gctcgtttgt
    12961 gatgttgggg tgctttctgg taggaatacg agaaaggttt acaacatcaa aatgcttgtc
    13021 gaggaaacgc tcggtttcaa ctcttgcctc gttcgagtca tagaaaatgt ggaacgcgta
    13081 ctgatccttg tattcgggat aaatgccgta tcctgcgaaa ccgcctccca gaccgttaga
    13141 tctgtcgtgc atatatgata tcgactttac tatcttgtca ccgcatattc tgttgccgtc
    13201 cctgcttata aagcctgaaa tagcacatcc ggcgggtatt ctgacctgac cttctttttc
    13261 cagcataagc tcactcctgt tcggtatagg ttttgttttt tgtgttttga gcgttttaaa
    13321 tccgtcaaat ttcatattcg gggataaaaa cagcaggcaa cagatgattt atgccgtaaa
    13381 ttcgcggtat tttgtaattt tgccgtaaat ttaaggaata tttgcagtaa aatgaaactt
    13441 tcgtttcaat ttgactgtca tctgaaacag ctttgtttcg taattgcctg cagtcgtttt
    13501 gactacagtt gttattatat cacggcttat cgatgttgtc aatatgtttt tgcaagattt
    13561 ttaaatgaaa cttgcaaaat gttttcttgc aaatttgcgg ttcgattatg caaaatgaca
    13621 gcagatatac ggaatttggc gcagtgatgc cctttttgca agttatacga tttcggacaa
    13681 agtaataaaa ccttctctgc cgtcatatat ctgtactaaa aacgaaagca ggtagcggta
    13741 tggtaagacg cagaagttac aagtcggcga taaaaaagtt cagacctttt ataaaaggag
    13801 ctgtagccgg aacagtgaca gcggcggttt ttaccgttat cggtgcattt ttatttaata
    13861 ttgccgatgc tcccgagggc gcggatatca cattcggata catatcgctc ggtgcggcat
    13921 cgggcgtatg cggaatgttc tggggcggtg gaagaggtaa aaacggcttt gcgtggggaa
    13981 cgctgggcgg tgttacaata tttgcgctgt gttttatcgg agcgatctta tcgggaggtt
    14041 tttcgggtac ggcagtgatc tcaaagctgc ttacatctgc gctatgcggc tgtatcggag
    14101 gaataatcgg tgtcaatatt gtggctgagc gataaggact gccgatagaa tatttctata
    14161 gacaaacaaa gaaaaatgtg gtagaattac cttaaaccct gtagatttca gggtaattga
    14221 aagtcaaagg aagatttaag atgaaaaagt attcaataga aacgcttgcg gttcacgcag
    14281 gctacgaaac tgacgaagct acaatgtcgg taactccgcc cctgtacgaa acaaacgctt
    14341 atgtgttcaa ggacgcaaac catgcaagaa cactttttga gcttaaagag cccggcaaca
    14401 tctactcaag actacagaac cccacctgcg agatacttga acgtaaggtg gcggctatgg
    14461 acggcggtgt tgcggcactg tcgtttgcat caggtcacgc tgctatattc aacactataa
    14521 taaatctttg cagtgagggc gacgagcttg tttcgtccat aaacatctac ggcggtgcga
    14581 taaacctgct cggtatcacg ttaaagagaa tgggcatcac ggtaaagttt gtcgaccccg
    14641 atgacctttc cgcctgggaa aatgccgtaa cagacaaaac aagactgttt ttcaccgagc
    14701 ttatcggtaa ccccaacgca aacgttgccg atatcgaaga aatcggtaaa atagcgcaca
    14761 aacacggaat acctttcgtt gttgacagca catttacaac tccctatctt tgcagaccca
    14821 tagagtgggg cgctgacctt gttatccact ccgcaacaaa gtttttaggc ggccacggca
    14881 gtgtaatggc gggtgttgta gtcgacagcg gaaagttcac gttcaagggc aaccccagat
    14941 tcccgctttt caacgagcct gacgtatctt atcacggtgt tgtgtttgca gacctcggag
    15001 agctggcatt catatcaaga ctgcgtgcgc ttatcacaag agatatcggc gcctgccttt
    15061 ctgcgtctgc ggctcatacc tgccttacag gtatggaaac gcttgctctg cgtatgcagc
    15121 gccattgcga aaacgcgctt gcggtagcca aattccttga agcccaccct gccgttgaaa
    15181 aggtaaatta tcctgcactg ccctcaaaca agtattacga gctttgcaaa aagtattgtc
    15241 ccaagggcgc aggcggcgtg ttcacattcg ttatcaaggg cggcaagccc gcaggcgaga
    15301 agttcatgaa cagccttgag cttttacaga tagttgcaaa cgtcggcgat gtaagaagcc
    15361 aggttatcca ccccgcttcg acaacgcaca gccagctgtc gcccgaacag cttgtagccg
    15421 caggaataag cgagggtacg gtaagactta ctatcggcat tgaggatata aacgatatta
    15481 ttgcggacat ttctcaggcg ctcgataaag cgactaagta acataagatg aatatttaac
    15541 gggagcgggc actaacaacc cgcccccgtt ttttataaaa attttataaa accccttgac
    15601 aaactccttt cgatgtgcta aaatatatta gtaaatttac aagatatgcg ccaatagctc
    15661 agctggatag agtatctggc tacgaaccag agggtcgggg gttcgaatcc ctcttggcgt
    15721 gccaatttac attagttaca ctttcaggga tactacagta gtag
//
LOCUS       DAWAOG010000005        11950 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_944_length_11950_cov_2.348550, whole genome shotgun sequence.
ACCESSION   DAWAOG010000005 DAWAOG010000000
VERSION     DAWAOG010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 11950)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11950)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11950
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_944_length_11950_cov_2.348550"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    529..538
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3488..3497
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7800..7809
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3044 a         2844 c         2492 g         3540 t
ORIGIN      
        1 acagagcaac ggtgagagcg ttcttcgcca gcttcttttc tatcatttca gcttctttgg
       61 aaagcgcgtc ctctgtctgc ctgtcagacg gctcattcgg cagtgcgaca ggctctagct
      121 cgtatatttt aagcgtacag aacccaccga gccgcttttc atattccttg caggcatctc
      181 tgagatagct ttccttcagt ctgccgaggc aaattatatt tatgctctgc atcagcgttt
      241 cttagcgtag ctttcaaagc ggcgcttctc ctttgatgcc agctttctgc gcttcgagtt
      301 ctcgatagtt cttgtgacaa taaagcagat aagcagtatt acaattccgg ctattgaagc
      361 tttgagccac ggcttgtcga actcgtcctt tgctttttct atataatact ccacgtccga
      421 tcttgcaacg ttgttgtttg caacaaggtt tacggtagca atcgtttcac ccttgaattt
      481 aacgtccata acgccgagta tatcgccctt tttcacagga gcctgtatnn nnnnnnnntg
      541 gcataagcgc cgtgtagtcg ctttcgggag ttaatataac gtgtgtcgcg ctctctccca
      601 tctcaacctt gacctcggtt atctgctcgt ccttgttcac aacggtagcc atttcaaagt
      661 ttgtgaatgc ccacttgtag aggttgagcg cgtccaccat cgaataccag tcgtcatagt
      721 attctccgtt cttgtccttt accggtgcgc cgagcgtcac aagtatatag ttgtactgat
      781 ttttggcggc tgaaacaagg catcttccgg cttcatcggt agtacccgtc tttgtgccgt
      841 atgcgtattc atattcataa acactgccgg gacgcaggag agaaatagtg ctgtaaatag
      901 agtagccgtc ggggtttgcg tcattcttcg gcatttcgta ttcatatgtg cttgttattt
      961 ccttgaacag aggaaatttg tcataggcgt actttgtcat cttgtagaga tcttccgctg
     1021 tagtgtagtt gttgttatta tgcagaccgt gagcatttac aaagtttgtt ccggtacagc
     1081 caagctcctt cgccttttcg ttcatcattc cgataaactt tgatatgctt cctccgccta
     1141 tgttgtaggc aagaatattt gccgcttcgc aggctgacgg gagcataagt gcgtataagc
     1201 agtccttgta tgtcaggttg tcctgcaaag gctctatcgc cgcatttgac ggatcgctgt
     1261 agttcggatc ctcatagaag tcgttgaagc agtcataagg cacttcgacc ttctttttaa
     1321 gagtgccctc cgacagatgc tcaagcgcta ttatgcaggt cattattttt gtagtcgatg
     1381 ccggatacat cttcttgctt tcgttctttt taaagacaac atcaccggta tcggtgttta
     1441 ccatatatac agcctcgctg tatatcttat cggtattcgg atcaaaatcg tatacttcat
     1501 catcgccgtc ttcggcaaaa gcggtaaaag aaagcgagca taacagcatc gccccgcaaa
     1561 gcagagctga cgcaaaacgt aatatattct tcatgattta ttccattcct tttcatcgga
     1621 gatttttaaa tatgttcttt attattgtat cacatatcgt ctgaaaagaa aagctttttt
     1681 tcgtttttgc cgctttattt tgcatattct ccttgtcggt ttctttttta tgtgttaaaa
     1741 tagaataaga agtttcttac aattcggcaa gaaaaccata tcagccgaaa aagcggagta
     1801 aaatacaaag aggtgatagt atgaacggaa atgtgcggag aaatgccaaa acagcttttg
     1861 tctgtgtatt gtttataata attgtttctt tgttttttta tcttcttacc gcaggcacaa
     1921 tgccgaaata tgtcgatata ccgtccgctg acaaaatagc tgatgccgat ttgtcaaagg
     1981 agggtgtcgg tatagatata tcctccggcg aatactacac aggacggctg tatacctcgg
     2041 acgattttgc aaaaggcaac atatcacagg gcgatcccga tgataaatac gccacattcc
     2101 gcatagtgct gccccttgaa aaaggtatca cctacggact ttccggagaa acggcgacct
     2161 atgcgcaaca ggtttatatt gacggaaaac tgtcaagcgg ggtcggtaag gtgtccgaca
     2221 atgccgaaga atttatccct gcgacagaca gatttacact atattttacc gcataggcgg
     2281 ataccacaga aataataatc cgccacgcat ggttcaatca caaaacaggt acgatacaca
     2341 agctctgcct tgcaaaagcg ccgcttatat ccgcaaacga cagggcgcag accgtttgtg
     2401 acggactcat tgcaggaaca ctgcttgcta tggcgatatt ctttttcgga atgtttcttc
     2461 tcggaaacag aaaacgcagt atgatgtggt tttcactctc ctgtcttacg gcggctctgc
     2521 attatctgat atatgaaagc aagcagataa gggtgctttt accgcatctt gactggtata
     2581 cagagcatat aagctcgaat atatcactaa tatcctctat ttcgtgttta tactgcttta
     2641 cgcctttgct atgctcggat taagaccgcg taagcggttt gtggtgacct cggtatgtgc
     2701 ggtaggttcg atattactgt tctacatcat aacgccgtca accgtttaca cacattttac
     2761 cgtagtgaca ggcgctgtta tggcagtata tctgatcctt tcgtctgtat atatctgtat
     2821 tatgaccgta aagcacaggc tgatgggcta tatcgacagc attatagtat gctctaccgt
     2881 tgtacttacc actgccgtgt atctgataga agggcttaca tattttacaa aaatattcta
     2941 tatccgctcc tatgccacta tactgtttgt attctgcaat gcggtcatgc ttacgataaa
     3001 tctttccaga acggagcgcc gccttgacga gtcgaaacag cgcgaattgc aagttaccca
     3061 tatgaatgaa actctcgaaa agctcgaccg gctgaaaacc gattttatgc ataatatcgc
     3121 ccatgaaatg aaaactccgc tcactgtaat gagcggatac gcacagctta ccgaaaagca
     3181 gatagacaag aatatcgtga acagcgaaac gctttcaaac ctgaaaacca tttcaagcga
     3241 agctcaacgt ctgtccgata tggtaacaaa gctgctctcg gtatctcata acgaggcggc
     3301 tgcaatagaa ctgagccggt ttgccgcaaa ggacgttctt tcagatgcgg cggcggtgtg
     3361 cagacctata cttgccaaac acggcaaccg gcttgtcaca agggctgata actgccacga
     3421 tattatagca aaccgtgaaa tgcttctgca ggtgcttatt aacctggctg taaattcagg
     3481 caagcatnnn nnnnnnngtt tatagtatcg gataccggaa cgggtatatc ccgcgaggca
     3541 ctcatgcact tgtttgataa aggctacagt actgacggcg gtaacggtct ggggctttat
     3601 atctgccgtg atatcataga atcaacaggc ggagaaataa ctgcagacag cagtgaaaac
     3661 ggtgcggtat tccgctttac cgtaccatgc ggaaaggaag atgaaaagtg agcacgattt
     3721 tgcttgtgga ggataatccg catataatga agataaacag ctcgtgtctt tccatggagg
     3781 attacagtgt acttgaagcc gccgatgcgg cacagtgtcg caagctgctg tccgagaatg
     3841 atgtcgacct tgtgatactt gatattatgc tccctgacgg tgacggagta tcgctctgtc
     3901 gtgagataaa ggcaaaatac gatattccta tactgtttct gtccgcgctc agcgaaaacg
     3961 aggatattat aacagctctg agagcaggcg gcgatgacta cctgccgaag ccgtacgata
     4021 tccaggtgct tatagcaaga gtagaagcaa gactgcgcgc agtccggaaa gacaagcgat
     4081 atatcacttt cggcagcctg aagcttgata cgatatctat ggcggcatat tttgcagaca
     4141 acgacctgct tgcgacccat aaggagtttt cgctcctgct gatgcttgta cgcaatatag
     4201 gcagcacagt gcaaaaatcc gagctgtacg aaaacgtatg gggactgccg tattacgaca
     4261 acgcaaatgc actgttcacg gcagtttcac gactcaacaa aaagctggac gataataacg
     4321 cagatgtaac tattacctat gaaagaggta aaggctacgc acttgaaaag atataaagaa
     4381 aacaatgact tgatttttat atcagcgcaa tatgccgtat attgaagtgt tgccccataa
     4441 aagcgctatc gaaagccggc tgtcacagca aaagctcaaa caatataaca accggtcggt
     4501 ttttcggctg aagccatcag cccgacccgc ataccctctg ctctctcaat ctctctttga
     4561 taagctctgt aaattcatcg ggactgataa cctttacgca cggaccgaac gacagcacac
     4621 ggataacgat ctccgtttca tcggtcgcgt aatatttcag atgaagcaga tacttattat
     4681 cgtcaacacg ctccgcttgc ttttcaaagt gcgcaaagtg gagcattaca cgctcgagcg
     4741 cattccgctc gtcatttatc tgcagggtaa gctctcttat ctgcattgtt tccggctttt
     4801 cgttccacgg acctgtgcct gcgtacagct gacagtttat aattcttcca agatttatct
     4861 gtctgaactt acagccgtcc attattactc ttatcttatc gtccttttcg gaatattcaa
     4921 accctttggg aaaaaatctc agcgttacct ccttgccgta cctgttgtca aaccttgcga
     4981 ttatcggcag atcgttcttt atggcataat atatcagcct gaaccgaccg atatagccct
     5041 catcgccaaa atcatcaccg tccgagtatt tatcgtatac tttataatcg tcctcggtga
     5101 aaagcggctc gacatcggat aagtcgggaa actgtacatc gaacagcctc accctcttat
     5161 caagcgatac ggctttcagc cagcgtttct gaagcaatgt cagcggcatt gtcggctcgt
     5221 gctttaaaag cggatttaaa ctgcgatcaa gcagcttcca ttttccgctt tttagcgacg
     5281 gccttattgt aagcacgctt tcggaaaatg cgttttcgct gacgatacgg tcaaggtctt
     5341 tttcggtcgc gctttcgtca aatgcggcgg tgattatctt cgctaccgtg ttataatacg
     5401 ccgagtaaag ctcactgaat atcatcgctt tcacctccgc tgtcagccgt gttatcctct
     5461 atgccgtata tttcatacat tttctctata tcggctctga agctgttctg taccgtctta
     5521 tccgaaaaat cgagccttgt tatacggcag ataaacgtgc gtatccacgg tatcatttcg
     5581 gtagtgtcga atacatcggc ggagtatctg taatggttgt cgtcgatttt ttcaacctta
     5641 ccgcaccgct tttcacgctc aaggcggttt actatatact gctcgttgtc tgttattctt
     5701 atatcaaact caacgtgttc cgtgctgtcg gtatcaagcc tgccgcttac gccccacata
     5761 cagctttcag ccgcattcag cttctccctc agctcgtcaa acctttcgca cggctcgccg
     5821 attttcacat cggatatgta atcaagccga tacgaaaaca acctgttcag ctttatatta
     5881 taggctataa ggtgctgtct gccgttctgt gcgctgatat agatccggag cggtacaagc
     5941 cgtaccgaca tcggcttgtc gcagtgtctg ccgagggttt ccacggtgac acagcatctt
     6001 ttctgcattg cgtcaaaaag cacagccgct atatcgctgt caagcgcctg cgtgatatag
     6061 tggtgcttga atgtgaaaag cttttcgtgc ggcggtaatt tgtccgagat aaacgagccg
     6121 ataacaccgc acggtgcggt ttctccgaaa aagtcgacaa catcgcatag ctgtgacacg
     6181 tcaatatcgg gagaacggct gtaaagtacc tttctgccct gcttttcggt tatgataatg
     6241 ccctcctcgg tgtattcctt aagctttttt ctgagtgtcg attcgtcaaa tgtaaaatca
     6301 cccgacagca aagtatcgat ctcatctata agctccgaca gcgtcagctt tatatcggga
     6361 gtgtaaagta tatcgaatat cgcaaagtga agcgttatat ccccgtctgt aaagctcttc
     6421 gctttccacg ctttatagaa agggttgctg tcctcgttac ggctgtctac cgacagaaat
     6481 acattcttgc cctcggcggt cttggtaaag cgcatatagt ccccgagcca gctttctacc
     6541 ctgcggcgct catcgtcata tgagcgggcg cttttcctgt cgtattcgtt tctgctcttg
     6601 aagccgtata catagaactc cctcatatac gcgcgtattt tctcaaagtt ctttataagc
     6661 tcgctgtatg ccactttatc acctctgtca tatgtaagta atacccgact gctttcacag
     6721 tcgggtaatt ctgttctgtt ttatgcgcca agctccagca caggcgcagt gccgatagta
     6781 taaccgtctg cactcactcc cgctgtcgct ccgtcccacg cataggcggt gatctcatcg
     6841 ccgttgccat tgacccacgc cttgcttgtt ccaaaagcgg gaacatcgtt tatatcggag
     6901 atattcgtcg tatcgggcgt gtaccacgcc gatacgcaca cgccttttat gcagttggct
     6961 cttatcacgc cgtttttaag ctcgggaaag cagttaagca gatatgaacc caacagctct
     7021 tctccgtatt gcgatgtcat cgtcttgctg tccttgtcaa caacagcact gttgccccac
     7081 gttaccgtat caggcgaaaa cgcagacgga ttttgcacag cgtcaatatg ccattcgctg
     7141 tcaactaccg ttatagtgaa gacctcacgg caatcttccc ccataagcat atagttcttg
     7201 cggcttgcgt ccgccatagt cagaaaatag cctatctgct ttttcatttc tgcggcagtg
     7261 ctgttggctg agccgacatt agctcttatt gtaacaccta acagtacagg cacaagtatc
     7321 gcggcaagca caccgattat agccataacc acgacaagct caactatagt aaagccttta
     7381 ttctgatgta agcgccgaat agtattcatc atatttcctc cgtgttagct tattaagtta
     7441 ataataacac atttcacgca gaaaatcaac agatatataa taatattctc tgtttatatt
     7501 attgtgaatt taccccttgt ccgtttttat actcatgctg tgagtgcaca agcaggtact
     7561 cgtctatgca ttcgggcggc gtatcaagcc agcctatata ataggcggtt atgttggtta
     7621 tctcctgcgt tatacggtcg ggtaagcgct tgcccgtttc acggtcatag gttgagagca
     7681 taatgtcctg cggaacgttt ttcctgcaat gctcaataaa atctacagtt accccgtaat
     7741 ttatatcatt tgcatcaaac gcatacaggt caggcgcacc gtaaagatgt attatttcan
     7801 nnnnnnnnna tcatcatttt cggtatcatc atcatactcg tttataaaca agagatataa
     7861 aaagttgtca caattatact tatttttcag tgccgcactg tcgattttgc cgtctatata
     7921 ctcccaacat tctgtgttat cgggattttc tgttatatta catattatgc ccttaaaatc
     7981 ggcttcatag tacagatcac ttttttcatc agtcggatat acaagagaaa atgtcttatt
     8041 ataattatct gcctgtttgc cgagccactc ggcggcaatt ttcaacgcgg caaaatcacg
     8101 ctcacgcaac gctctgtcgt tttcgttacc gaaatcccag tttgattttt catcaccgca
     8161 aaatatagtt acaacaagcg aattgccgac cggcatatct gccgagccta aatcgtcaag
     8221 ctgtatcgtc ttttcctgct tcggcacagg tcgcgggaat attctgacaa cggcaaacct
     8281 atccagcata aaaatcactg caattgcaca tataattatt accgccacac cgattatcag
     8341 cgttatttta tcttttttct tcatttctta ccgtaataaa tatcccccga cagtcacccg
     8401 tcgggggata tcaaattcat ttagcaattc ttaagtaaaa tacttatgat gccttaccaa
     8461 gcttaagctc aggagctgta ccaaggatga taccatcaga tgtgatacct gctgtgttac
     8521 cgtcccaagt ataaacgccg ttcttgaaat cgtcttctgt aggcattgaa gaaggtgcat
     8581 cagtaccatc agcagagtat gcaacatact tgcatgaacc gcctacgcag tatgcgaata
     8641 cataagagct cttaacatca gggaatacat cagcaagagt tcttgtcata agctctgttg
     8701 catcttcgat gtcagactta ttaccttcgt tagccttaac gcctgttatt tcagtacccc
     8761 atgtctttgt gcccttgaat gcatcagaag cggcaggaga atatgagccc tctgataatg
     8821 ttgttcccca tgtaccatca tcagcgattt tgaatgtgaa gcttgctata gcggtgtttg
     8881 acttcttcat gccgtagcct gctgtatcag catctgttaa gaagttgttg atctgcttct
     8941 ggagttcagc tgctgttgtg ttagcagatg ttactcttga agaagtaaca aagcccagca
     9001 ttgtggggat caggatagct gcgagtacgc caatgatagc gataacaacg atcagctcga
     9061 ccagtgtgaa gcctttcttg gctttcagtt tctgtaattt ctttatcatg ggaaattacc
     9121 tccttgaaaa atgtaaaagt tttttatgtt taacggtctg cgccgttaaa tccaaaatcc
     9181 tccgagagtg tctgtcacct taagtctatg tcggaagctt cactcttagc actttacttt
     9241 aagctgcatt gccttaagct ctgccgtccg catctttaag atgtttccct tagcaccctt
     9301 ggtgttttct gtatcctaag gataccacaa cacattcaaa aaagcaatag ccaatttgct
     9361 aaattttgtg ctaatcttta aaattagtga attttagata taagcgattt ctttttgtgg
     9421 tgcattttgc acaaggattg tgtaactggg ttacacaaat atcggcttac gctcatatta
     9481 tgctgtcgtc aagcagaaaa tccactattc ctatattaag aactccgctt tcattatacc
     9541 aacgcttagt tatatcgtga cgtactatta tcttaggaaa atagtcaccc gtcagcgaaa
     9601 acggcttgtt ctcctgcatc gtcttttcct catcgggcaa cgcaaaggca gactggatat
     9661 acagcttttt ttctccgtca tttgctataa agtcgatttc cctcgcaacc tgctttgctt
     9721 ttccgttgtc gtctttctcg cttgaataca ccactccgac atctaccata cagtcactga
     9781 taatcagctc attatatatg atgttttcca taatgtgcgt catttcctgc tgtctgaaac
     9841 ctatccgtgc attcctcagc cctatatcct cgcaataata cttgttcgga taatcaaaat
     9901 agcctttacc tctgacatcg taccgcctgc attcgctgaa aagatacgcg tcggtcagat
     9961 tatcaatata ctgctttacc gtattattag cgactgagct ctcgcccttc agcttctgct
    10021 ttgaattaag gctgtttgca atgttggtcg ggtttgtgag cgaacctaca gacgagcata
    10081 acaaatcaag cactgccgac aaaatatcct cacgctttat tctcctgcgt tcaacaatat
    10141 ctttaatgta gacctccgaa aacagtgtac tcagatagtt catctttgct ttatcggtag
    10201 gtcttgacag cacaagcggc ataccgccgt aaaaagcata gttgtcaaac gcttcatact
    10261 tatctccgcc cacagcggaa taatactccg cgaagctcag cgggtgcacc cttatctcat
    10321 cgcttctgcc tctgaactgt gtgagtatat ccgccgacag catttttgaa ttgcttccgg
    10381 tgacgtatat atcaaggttc ggcattgaac gcaagtcgtt cagagcgtca tagaacgtaa
    10441 tctttttacc gtccttattg tatggattcg gcacttcgtc cgacatctgt atttcgtcca
    10501 caaacagata atactgctca ctgtccgtcc ttactctttc acgcacggct ctgacaagtt
    10561 caagagggtt tctgtatttt atatcacttg caaggtcaaa tgcgtaggaa aagatatttt
    10621 ccggctttgt tccgttttca agcaggtact ttctgaacag cgtaccgaga agatatgatt
    10681 tgccgcagcg gcgtattccc gttattactt tcacctgtcc gtcccacata tatgaaatta
    10741 tcttttcaag atacctgtca cgttttatct ccataataca cctccgctga aaacttacca
    10801 taaaaattgt gctacttttc aatatgatta tacaccgttt ttagcaaaaa ggcaaggggt
    10861 tgctttatct tcactgaaaa cttcgtcgaa aatacgagta agttttcaat aaaaccgctc
    10921 ttataaagca agaaccccct ccggcatatc gcctgagggg gttctctata ttagtttaaa
    10981 acattatgga gcttgcgatt aaactgtttc gtcaacgccg tagtaagcat tgaggtacat
    11041 ctgcttgatc tcgctcatca gcgggtatct ggggttagcg cctgtgcact ggtcgtcgaa
    11101 tgcctgctct gtcatagcgt caagtctgtc taagaagtcc ttctcatcaa taccgtagtc
    11161 cttgattgtc ttcttgatgc ctacagtttc cttgagctgt tcgatcttag cgatgaactt
    11221 ctcgaacttc tcttcgttgt tcttgcctgt tacgccgcag aattcagcaa cttcaacgta
    11281 tctctcaaga gtatgggggt gatcatactg tgagaatgta cccatctttg tggggttggg
    11341 aactgcgttg aaacgcataa cgtgtgtgat aagcagagcg tttgcaatac cgtgaggcag
    11401 gtggtggaaa gcaccgagct tgtgagccat tgagtggcag acaccgagga atgcgttagc
    11461 gaatgcgata cctgccatag cagaagcatt agccatcttc tctcttgctt caacatctgt
    11521 ctgaccttca gcgtaagctc tgggcagata ctcaaagatg ttccttgcag ccttgagagc
    11581 aagaccgtct gtgtagtctg tagccatgat cgatgcgtaa gcctcgaggc agtgtgtgag
    11641 tgcgtcaaca cctgaagcac ttgtcagacc cttaggctgt gtcatcatgt tgtctgtatc
    11701 aatgattgcc atgttgggca gtaactgata gtcagcaaga ggatacttaa cgcctgtctt
    11761 ttcgtcagtg ataactgcga aaggagtaca ctcagaacct gtacctgaag atgtggggat
    11821 agctacgaag taagcctttt cgcccatctt gggaaatgtg taaactctct tacggatatc
    11881 catgtatctc atagccatat cgaggaagtc aacatcggga tgctcgtaaa gtacccacat
    11941 gatcttacct
//
LOCUS       DAWAOG010000006        11856 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_948_length_11856_cov_2.370731, whole genome shotgun sequence.
ACCESSION   DAWAOG010000006 DAWAOG010000000
VERSION     DAWAOG010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 11856)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11856)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11856
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_948_length_11856_cov_2.370731"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1723..1732
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4918..4927
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5829..5838
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3397 a         2725 c         2924 g         2780 t
ORIGIN      
        1 aaatggactt tttctacaag ctgtgacaat atatttattg tcttgacttt tttataatat
       61 gttgcactgc aatttcaatg aagttccgat aaaataagga gctgtgatga aatttttgca
      121 tttcatcaca gctccgttta ttgtttactc aacctcagtc aaagcttgtt atctgaggat
      181 ttctgcaatg ctgaagaaga tacagtactt cttcgctttt tacaaagtac gactttgaat
      241 aagcatcgtc aacgtaaaag tcaagcagga acttatcctc ccactcgtgg agcgatacct
      301 tgagcttatc ctttatctca tcgtacttgt cgccaaacag agctctcagc cctctgcccc
      361 tgcgcaaaaa accgtatacg aacattgctc cgcgaaagca cggcagaagc tgtgagtaaa
      421 tcggtgagtc acggcggaac tcgatcacca ctttctgctc ggtaagtcca ccgggcgcat
      481 ctctgtcctc ggggagcact gtcctgaact tttcgggcgg cattattgag ccaagctcga
      541 taaattctgc attttcataa ggtgctgtca taatatatcc ttcctttcgt cttcatataa
      601 aactatcgat gtctttatta tatatgcgcc gtaccctttc ttcaatgagc ggacgcacaa
      661 tatacttaaa ccatagcaca gcttacagga actcttttat aaagaatgat agcatgagcc
      721 tgagcttatc ctcgtaattc agctcgcagc cgagtatttc ctttatgcgt cggagcttgt
      781 aatttaccgt gttgcggtga acgaatgtca gtcgcgcgac ctcctgtaca ctactgtcgt
      841 tttcaaggta gcatttgagc gtttgcatat agtccgtgcc gttcgcactg tcgaactcct
      901 cgagagtgcc gagcgtttcg tcatatatct catggagcac tgccgtatct tttatatgta
      961 ccagcagttt atatatgccc atatcgctgt agcctacgca tttgtcgttg tggagcgttg
     1021 ccaccttgag tgccataacc gctttgcgat atccgtcgga tattgcactg tagccctgcc
     1081 ttgacgggct tatgccgcaa gttgtctccg cgccgtatct gcctgcattg aaaatcgtat
     1141 caaacgaggc aaatatacgg cgtacatcgt cctccttaca gtcggggcaa acaacaacga
     1201 ggtttttatc ctggaagaaa aagctgaact gcctgccact tgtattgagt accttctgca
     1261 tatcgcttgt gaccgctttt ttaagctcat catcgcctag agttgtcttg gtgaagctgc
     1321 aaacaccaac gcaatattcg gatgcggaaa aataactgcg ccgttcaagc gtggatttgt
     1381 atcccgcttc attatcgggc gaaaatatga gattgcgcag agctgttgca agtgccgttt
     1441 ctatttcttc tccggtcact atcctgtggc agaagtcata ggtgatatca atgagcttga
     1501 cctcccacgg aacggtaaac aacggcaacg aattaaggtc gcaacagtcc ttgacgctct
     1561 gcggtatact ctttatatat ttaccggtat ttataacaag tgcactgcat cgcttttcga
     1621 taagctcttc tataaaggta agcagacggc tttcatcgct tatgcccact cctgtcataa
     1681 agaccatttc atttccatgc aggaacgggg acacatcgac gtnnnnnnnn nngagataga
     1741 gtatagcaca gttttgcata attgtcaaga gaaaattgca aaatccaact acagcacaaa
     1801 caaattatct ttctttaagc actttgcaca attcatctaa attgctattg caatgcgatt
     1861 ttaagcgtga tataatgcaa ttacaccgaa agaaaacagt gaaattaaac aaaaagaaac
     1921 ttgcaacggc gcagaaagag atttctgtgc cgtttcgttt tgcaggaaca aaaagataag
     1981 acttgcaaaa caggaaagaa aggagtgctt agatatgagt gaaaaagtaa acgcaccgga
     2041 gatttcggta aagaacgtca gcatggtgtt ctccgacagt aaaggaaacc cggtacaggc
     2101 acttaagaat gtaagcatcg acattcagaa aggtgaattc atctcgctgc tcggaccttc
     2161 cggttgcggt aaatccacac ttcttcgtat agtatcggat ctgctgacac cgacttcggg
     2221 cgaggttacg gtttgcggca agacgccgag agaaacaaga cttgaacaga agttcggcat
     2281 agtattccag aaccccgtac ttctcgactg gagaacagta agaaagaaca taagacttcc
     2341 tcttgagatg atgaaggttc cgaaagaaga gcaggcaagc cgtatcgaac aggtactgga
     2401 gcttgtcgga cttaaggagt tcggcaacag ctatcccgga cagctttcgg gcggtatgca
     2461 gcagagagtc ggtattgcga gagcgcttgc gatacagccc gacatactgc tgatggacga
     2521 accgttctcg gcacttgacg agttcacaag agaaaagctc aacgacgatg tactttcaat
     2581 atggagaaag acaaacaaga cggtgctttt cgtaacacac aacatatccg agtcggtata
     2641 tatgtccgac aggatctgcg ttttatcacc gcatcccggc agactttcgg caattgttga
     2701 tgtaaatctt cccagaccga gagttccgga tatgagaggt actcccgaat ttgccgctct
     2761 tgtagagaag gtaagaaaca gcttcgaggg tatataaaac cgaaaggagc gttaagaaat
     2821 gataaaaagg ataacgtcat ccaaaggcat agtaactgcc gtatgggtgc tcggacttat
     2881 agtaatctgg gagatatgcg ctttcattgt aggcgcaaca cagcgtacac ccgtaaacgt
     2941 tcttccacac ctgtatcaga taatcggctc gttctttgat acaaagtcga taaccggttc
     3001 gggcgatacg atagcaacac tggttttctc cagtgccgct gaaacgcttt caagggcgct
     3061 tatcggtttt ataatcggta tggtgctggg ttatgtactg gctcttctga tgcatatgtc
     3121 aaatatagtc gaaaagatag cgttcccgta tctgatgata atacagatga taccgatact
     3181 cggtatggca cctatcgtac tttcgataac aggcgatatt tcaagcgcac gaatcataat
     3241 cgccgcaata cttacattct atcccgtttc gacaaatacg ctggcaggct tcaagtcggt
     3301 cggtcgcgaa agacacgagc tgatgagatc ctacggcgcg aataaatttc aactttacac
     3361 aaagatgctt atcccgtcag caatgtccta cttcttcacg ggtcttaaga tttcagcgcc
     3421 tatggctatc acggcatcga tactcgttga cacgctccag ggcggcaacg gactcggctg
     3481 tatgctctcc cagtcgctca aaggctcaat gacacgattt gtattctggg atatagtaat
     3541 tttcagtgcc gtcataggtg tgctcagctt ctatctgatg ggtgtcatcg aaagagcgac
     3601 aaatccgaca aaacgtaaag taaagaaaaa agcgcagtaa ggaaaggact gatcgtactt
     3661 gaaaagcaaa gcaatcaaaa atgtactcac aacagtcggt ctgcccgtac tgttcggagc
     3721 attgatattc gcactgtggc agacaaaggt tctgcacgcg ctgttcggaa cggacgagat
     3781 aatccttccg ctcccgacaa gaataggctc gatcatcggc gataacttca caaatatgtt
     3841 accgcagata gcgtcaactc ttatagtggc gctcggagga cttgtactcg gctcgatact
     3901 cggctacttc atcggcgttg ccggtgcggt attcaagaag tggggcatgg gcggtctgac
     3961 catagtatcc gcattcaacg cgatcccgct cgtagcactt gctccggtta tcaacaactg
     4021 gacaagggat ctgtcaaacg tggtcgatat aagaagtacg gtggctaaaa taatcaccgt
     4081 tacgatattc tgcacagctt caatgagcgt aaacgcatac agaggtctga cggaggtaaa
     4141 accgttctcg gaggatctgt ttgcatcata cggttcaagc aaactgaaaa tgttcttcaa
     4201 gctgaggctt ccgaactcac tgccttatgt attcacagct ctcagagtaa gcgttccgtc
     4261 ctgcataatc ggtgcggtgg taagcgaata ctttgcagag caggtgatcg gtgtcggcag
     4321 acagataaga gagaacatag tcaagggtca gttcccgaca gcgtgggcat atataacgat
     4381 agcctgcgta atgggtatac tgatgtacat catactcctc tcagttgagg cgatcgtgct
     4441 gaaaaaccgc cgtgcaagat aaggcggttt cagatagaaa ggtgtagaca caccggctaa
     4501 aacctatttt taaaagacga gaaaggatga aacattatga aaaacaaaag agtgttagcg
     4561 gcattactcg cagcaagttg ccttgcaaca tcattcaccg cttgttccgg tgacagctca
     4621 tcaagcagca cagcaggaag ctcagccgca tcggcagatg cttcttcaag cgccgcagag
     4681 ggttcttcgg aaagcacagg tcttatgagc gatgaagaga tcatcaagaa ggcttcgagc
     4741 gaaaacaagg tcggcaactg gggtctcgga aacgagtacg aaattcaggc tctgctttca
     4801 aagtacggtc tgcccaccga ctacattaca atggacttca caatggatca gatagataag
     4861 gatactatca cactggcatc ggcaatgaca ttcaatgagc ttggtcttat caagaacnnn
     4921 nnnnnnngaa caaccccaac acagtaaagg catttgttgc cgcatcaatg aagggctgga
     4981 catatgcgtg cgagcatcct gatgaagccg cagaaatcgt attcaagtac ggctcatcgg
     5041 tatcggctga ccaccagaaa tatatggctt cagaggttgc aaagcttgta acgacagata
     5101 caaagggcaa tgcggtttcc gcttcggatg taggtaagat ggacgaagag gcaatgcagc
     5161 agactctcga ccttgctaag cagtacatca agctcgacga cgctacagcc gcagacaagc
     5221 tcactaagct tacacttgac gatatccgtt cttccgacta tctgacatat gacggcggag
     5281 cagttgagaa gtctgacctc aaggttcagc ttaaatggtt accgcaggct cagttcatgg
     5341 gctattatgt agcacttgac aagggctact acaaggataa cggccttaac gtagagatcg
     5401 taagcggcgg cggtgacgtt tctgaaacag ttgcagtaag caacggtact gttgacttcg
     5461 gtgtaacgtg ggtttctaac cttatcaacg ccaacgcagg cggtatggag cttcttgaag
     5521 tagcccaggt atatcagaga tcgggtcttg tactttgcta caagaagagc cagttcacta
     5581 agtaattcac ggtttcggat cactccggaa gctgaaaggc ggtatatatg agcgaaaaga
     5641 aaatacttgt aagcggttgc cttttcggct ggcattgccg ctatgatgac ggcgatacgc
     5701 cgtgtctgca cccacagttc cttaaatgga aggaggaagg cagactgata ccggtatgtc
     5761 ccgaagtgtt cggcggtctt ccgaccccac gtcccgactg ccagcgtgta ggcgacaagg
     5821 tgatgagcnn nnnnnnnngt gatgagctgc gtaggcggcg actgcaccga gcagtataca
     5881 aaaggcgcaa acgaggcgtt aagacttgca aaggaaaacg atgttatcct ttgcataatg
     5941 aaagaggaca gcccctcctg cggaagtacc cacatatatg acggcacctt cacggatact
     6001 atcatcgagg gacagggact tgcggtcgaa tatctcagaa acgcaggcta caaggtattc
     6061 agcgagaata tgatcgaaga tgtcataaag gcactcgcag agtaaacact acagcaactt
     6121 atacagtaag ttcagtatcg cacgagcagt taaataccgt tcgtgcgatc gggaaagacg
     6181 tcagggcgga cgtatcacaa catagcggtc gtttcgctcc ggcgtttttc ccgataaaaa
     6241 tacaaacttt atcaagagca gaaggaaaga gggtataaca gtggtattca gatcaccacc
     6301 aacgatcatt tacggcaagg gtacagttac ctcagtcgga gagcaggcgg cacgcttcgg
     6361 aaaaagggcg atgcttgtct gcggaaaagg ctcgctgaga aaaagcggtg tgcttgatat
     6421 catagtatca agccttgaaa aagcaggcgt ttcctgcgcg gtatacgacc gtgtaggctc
     6481 agacccgaca acatcggacg ttgacgaggg cgcggctttt gcaaaggaaa acggttgcga
     6541 tgtgatagta gcggcaggcg gaggaagtcc tcttgatgcg gcaaagggaa tcggaatgct
     6601 gctcacaaac ggcggcaagg tgaccgatta cgaaaagacc gctcccgaaa aggagtcgct
     6661 ccccattatc gcaataccga ccacagccgg cacaggcagt gaagcgacaa gatactcggt
     6721 aatcacagat accgacagaa atataaagat gctgttatca acagacggaa ttatcccgaa
     6781 ggtggcaatt ctcgattttg cgataacaaa atcattgccg tcatttatga cagcggcgac
     6841 cggaatggac gctctgaccc acgcaataga gagctacata aacgtcaatg catcgcctat
     6901 gacaaagatg tattcacttg aggcgataag gcttatatca aagagcctta taccggctgt
     6961 ccttgacggc agaaacgagc aggcaagaga ggatatgctg cttgccgctt tatatgcggg
     7021 aattgcaata tgcagtgcgc cgacagcgct tgtacactca atgtcaagac cgctcggagc
     7081 gtggtttcat attccgcacg gactggcaaa cgcaatgctg ttaagcacgg ttatggaata
     7141 taaccgcccg tcctgtcccg aaaagttccg cgacatcgca atagcgatgg gcgagaatgt
     7201 cgagggctta tcggtaagag aggcaagtat agcagctgtt gacgctatcg ccgcaatagc
     7261 ggacgaaacg ggactgccga agctgctttc ttctcaggga gtgacagagg acaagatacc
     7321 gactctcgca aaggacgcat tcgccgcagg aagtacggta aataacccga gagttcctac
     7381 ggtagaagaa atagaaacga tatataaaag cgtattctga taaaagaaag gaagaaataa
     7441 aatgagatac ggatttaaaa acgcagaaga ggtaaaggaa acctcactta agtacaacct
     7501 tcattcatgg agcgttcagg gcaagctgaa tcccgctgta gtagaaaaag cggagggtat
     7561 ttattactac accgcagacg gcaagaagat ggctgatatg tcaagtcagc tcgtaaacct
     7621 gaatgtcggc tacggcaaca aggatattat cgatgctatc aaggagcagg ctgaaaagct
     7681 cgcttacatc agccccgctt atgctatcga ctgccgttca aagctcgctg agatggtggt
     7741 aaaggtagcg ccaaagaata tgggcaaagt attcttcact ttaggcggcg cggacgctaa
     7801 cgaaaacgca ataaagatcg caaagcttgt aacaggcaga tacaagatat tctcaagata
     7861 ccgtgcatat cacggaagct cattcggcgc aggtaacctt accggcgaac caagaagata
     7921 cacacttgag cccggtatcc ccggcttcgt aaagttcaca gatccttatt tgtatcacgc
     7981 tccgttcccc tttgagagcg aggaacaggc tacagagtat tatctcggac agctccgcga
     8041 ccagatcata tacgaaaatc ccgatgcagt tgccgctatc gtaatggagt cggtaacagg
     8101 ctcaaacggt atcatcattc cgcccaaagg ctacttacag ggtgtacgca agatatgtga
     8161 cgagttcggt atcatgatgg tctgcgatga ggtaatggca ggctggggca gaacaggcga
     8221 atacttcgct tgcgaaaact ggggtatcga gcctgatatg ataaccttcg caaagggcgt
     8281 tacctgcgga tacgctcctc tcggcggcgt tatcgtaagc aagaagatag ctgagttctt
     8341 cgatacgcac aagctcgact gcggtcttac ttattcggct caccctctgg gctgtgccgc
     8401 aggtgttgcg tgccttaact tctatgaaaa agaacacatt atggataagg tcaaggaacg
     8461 cggcaagcag ttaggcacac tccttgagga tatcaaggca aagcacaaga gcgtcggcga
     8521 tgtacgttat atcggcctgt tctcctgcgt agagcttaca aaggataagg ctacacacga
     8581 gcctcttgtt cctttcggca aagaccccga gggtatcatg ggcaagatag taggaatgtt
     8641 aaaggcaaag ggcttcaata catattcaca cgaggcttgc atattcgttt gcccgcctct
     8701 tatcatcaca gaggaagagc ttgccgagaa tatggcaata ctcgatgaag ttctgacaga
     8761 agtagacaaa atggtctgat aaataacagc tattacggga aaggacggta agttatggat
     8821 ctcataatta aaaacggtac gatagtttcg ccaacggcta cattcaaagc cgatgtgtgc
     8881 gtggataacg gtaagatagt tgcaataacg gcagatgccg gcacagatgc cgacaaggtg
     8941 gtagacgcaa gcggcaagat ggtgctcccc ggcgctatcg atgcacatac ccaccttgca
     9001 atgcccttcg gcggcacgat ctcatcggac gactattacg caggcacaag agcggccgcc
     9061 tgcggcggta cgacaacggt attcgacttc atcttacagg atttcggtga aacgatggtc
     9121 gatgccatca agcgccgtga tgcattgtgc gctcccgatg cggctgtcga ttacgcattc
     9181 cacgttgccg taaaggacgt atcaaacgga cttatcgaca ctatagagga cgctgtaaac
     9241 tacggtgtat cttcgttcaa ggtatttatg gtctacgact tcggcgtaac agacggcgta
     9301 ttctataagg tgcttgaaaa ggcaaaatcc tgcggcgcta tgatctcggt acacgctgag
     9361 aacaagcagc tcatagacgt tctgacagag cgttatctgt cggagggcaa gacaagcgca
     9421 tggtatcact atatgagccg tcccgaattt gtcgagggcg aagcggacat cagagctatc
     9481 cagcttgcaa agagccttga ctctaagctg tacatcgtac atcttgcaaa taaggaaggc
     9541 gtgcaggcgg ttgaaagagc aaggaacgag ggctatcaga tatatgccga aacctgtccg
     9601 cagtatcttg aatttacctc cgaggtatac aagagggcag acggcagaaa ctttgtatgc
     9661 tcacctccga tgaagggcga ggaaagcaga cttgctctgt gggaggctat aaagaaaggt
     9721 cttatcacaa cgatagctac cgaccactgc ccgttccagt catacgaaaa ggactggggc
     9781 aaggacgatt tcacaaagat tcccaacggc tgtgcaggta tcgagaatat gtacccctat
     9841 atgctgtcag ccgcaaacga gggcaagata acattcaaca aggcagttga gctttgctcc
     9901 tacaatcccg caaagatttt cggctgtgac gcaaagggtg caattgaagt cggtaaggac
     9961 gcagatattg ttatctacga tcccacgaag gacttcacga tcactaatga caagatgcac
    10021 agcgactgcg accacaccat ctgggagggc atcaaggtca agggttatcc cgaagctacc
    10081 tactccagag gcaagctcgt attcaaggac ggcgagttct taggcgagcg cggctggggc
    10141 aagttcataa agagaagctc aagcggtaac ttataataaa caaaagccga tatataacag
    10201 gggagagcaa tatctcccct gtatctgtaa cagcacaaag gaaaaggaga gatacttatg
    10261 ccattcgtaa aaaatacagc tatagaagaa agcgctcgtt gccttttatg ccacgatgcg
    10321 ccatgtacaa aagcctgtcc gtgcggcgca aagcccgaca gatttatccg ctcattgcgt
    10381 ttcgataact taagcggcgc aaaagcgttt atgaaaaact gtgcagactg ctccgccccc
    10441 tgtatgaccg cctgcacaag agcgaaaata gacagaccgg tcgagataaa gcagacagcg
    10501 gagtatatcg cttctgccgc aaaggatacc gagccgaaag ccgacctgtc aatgaccttc
    10561 tgcggactta agtgcgaaaa cccatttttc ttatcctcat ctgtagtagc aagcagttat
    10621 gatatgtgtg caaaggctct tgatatgggc tgggcgggta tcgtatacaa gacgataggc
    10681 tttgcgaaaa tgaacgaagt atctccccgt ttcgatattc tgaacaagga aacaacaccc
    10741 tatataggct tcaagaacct agaacagatc tccgaccacc cgcttgagga aaaccttgct
    10801 atccttaaaa agctgaagga aaactatcca acaaagataa tagtatcctc aataatgggc
    10861 gaaagcgagg acgagtggac cgagcttgcg cggctgtcgg aagaagcagg cgttgacatg
    10921 atagagtgca acttctcatg tccgcagatg acaagtcata gtatgggcag tgacgtgggt
    10981 acaaatcccg aacttgtagc taaatatacc gccgccgtaa agcgcggaac aaagctccct
    11041 gtgctcgcta agatgacccc caatatcacc aatatggaga tccccgctat tgcggctgtt
    11101 gacgcgggcg ctgacggtat cgcggcaata aacaccgtca agagcatcac aaacgtcgat
    11161 cttgacagct tcgttcctac tcccgacatt aacggcaagt ccgctgtcgg cggatattcg
    11221 ggcaaggcgg taaagcctat cgctctgcgc tttatctctg atatgaaaag aaacgagcga
    11281 cttgaaagcg taccgataag cggtatgggc ggcatcgaaa catggcgtga cggtctggag
    11341 tttatactta tgggctgtac aaatctccag gtaacgacct cggtaatgca gtacggctac
    11401 agaataattg acgatatgct tgacggatta tcacgctggc tcactgccgc aggctatggc
    11461 agtgtatcgg aggtagtcgg ccttgcaaat aagaatatga ctgatgcggg cagtcttgac
    11521 cgtgacacag ttacatatcc tattttcgac aagaacaagt gtatcggttg cggcagatgc
    11581 tatgttgcgt gctatgacgc aggacatcag gctctcgact ttgacagtga aacaagaaag
    11641 cccgttttcc tcggctcaaa gtgcgtaggc tgtcacctct gcgctacagt ctgccctgta
    11701 ggctgtattt caaaggctaa gagggtcgca aaaagacgtt gattttttaa taaggaagtt
    11761 ttattaagag gctgtgacga aatgcaaaaa tttcatcaca gcttctcagt ttttcaaaac
    11821 ctcattaaaa ttacaatgca gtatattgta aaacgt
//
LOCUS       DAWAOG010000007        11287 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_990_length_11287_cov_2.520655, whole genome shotgun sequence.
ACCESSION   DAWAOG010000007 DAWAOG010000000
VERSION     DAWAOG010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11287)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_990_length_11287_cov_2.520655"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    627..636
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2882..2891
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3820..3829
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6652..6661
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9248..9257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2361 a         3069 c         2347 g         3460 t
ORIGIN      
        1 tagcacagcc gatggctgta atattcataa tttatatccc tctctcggga cgctgacgtc
       61 ttaacgattg tctgtattcc ttgatgagag aaagtatctc attcatcgac tgctcaacta
      121 tgtatgaatg acccgtactc gtggtaatta ccgtgtcggg attttccgtt actatttcaa
      181 taaagtcggg attaagcata aacttttccc cgttgagctt tgtaagtatt atcatacatt
      241 tccttccttt gcagactgct ttcgcttttt cattgatacc ggctgtctgt cacgccgaca
      301 gcttacacac ggcggtaaca gccgctttgc ataatataac gggcatcacg gcggagctga
      361 cgctccgcct gcccttataa ccttgaattt aaatcacaga accctgcaat ttctgctaaa
      421 ccgaggttac gtcaatcgca aactttcaga acaacgaact tgcaactgcc ctacacccac
      481 gcctttttaa taaccgccgc aggcgcacaa caattattca ttattcatca ttcattattc
      541 attaaggcaa cgcccccgcc cgccgtttcg ctatccgtcg ctacaccgca acgttattaa
      601 aggcggaatt tgccgcggca cattgcnnnn nnnnnngcac attgccgtcc gccggtaacg
      661 aaagttacgc cgctgtcaaa cctttagaac ggcggaactt gccgtgcccc acgggcactt
      721 agcgcttgag gtttactatt tcttcgagca ttgtgtccga agtggtgatt attcttgagt
      781 tagcctggaa gcctctctgc gtgatgatca tatcggaaaa ttcctgtgat aagtcaacgt
      841 tcgacatttc gagcgcacct gatactacag aagcagaacc gttttctccg gctatgttta
      901 cctggggctc accagatgaa agtgtttctc tccagtagga gctgccgacc tgctcaagac
      961 cgttggggtt gtcaaatgta acgatatcga ttctgccgag cattatgatg ccgtgtacgt
     1021 tgtgtacacc gtagatagtg ccatcctcat tgatatatac gctgtccaga tcctttaccg
     1081 tctgagtagc cgcaacacgt ccgtctgtca gcttgatgtt gcccagaagg tcgaccatat
     1141 cagagaagaa attgtttgag ccgcccgcaa gaccaacctt gccgtcagtt gcaagcagtg
     1201 tttcaagtgc accgttgcct gcatcccagt cggcactcgt aactttcatc tgatattttt
     1261 cgttgccgcc tgcagccttg tctatagccg cctgaatgtc agctaccgtt gcgccgggac
     1321 gaacacttat cgtaagaccg ttgtccgacc actttgccgt agtttctgcg gcattctttg
     1381 aatatcttaa gtttatcgtg tagttgtttg catattcacc cggatttacc gtagtgaatg
     1441 tgagcgaagc ctttgtcgtt ccgtcgtcca gcttcattgt gttggtagct gtcgttgcgg
     1501 gaatgtctgc cgcagaagga accgtatcaa gctcaaagtg gagcggaagt acgccctcaa
     1561 tgcttacacc gccgttttca cacgcacggg tcacagcatc ctcaagatcg ttaaggtttg
     1621 tataatcgtt cttttcgttc agctgtacaa caagcttatt gccgctcata taagcgtaat
     1681 cgctgtcggc ggtcgtaaat ccgatcgaga tatttccgtc cggagtcgcc gtgtcggcag
     1741 atacggtaag aggatatgta ccgcccttgt atgtgatcgt ctttgagtag tttgcctcat
     1801 tgtcgagaac ttcgggaact acaaatgtaa ttctgtttga agatgcgtca acgcctgtgg
     1861 catcgccgct tacgccgagc accatattac cgtttgagtc aacaaggttt cctgcgttat
     1921 cgacgctgaa tacgcctgct ctggaataga agatgttgcc cgcttcgtcc atgacctgga
     1981 agaagccgtc gcccgataaa gcgcagtcat aaacactgtc ggaatatgta aagcccgact
     2041 gtcccattac ctgaccgata gcgccgagct tggtaccgta acctacctga gaggggttta
     2101 caccaccctg gaggttagag cctgccgagc cgttcttctt tgtctggtaa tatacctcct
     2161 ggaaggtcgt tacgcttgtc ttgaagcctg ttgtatttac gtttgcgatg ttgttaccga
     2221 taacgtccat cttctgctga tgtgtcttta atcctgacac gcccgagaat aatgatctaa
     2281 ccatgatctg ttcctttctc catcgtgccg accgttctta cccgtctgtc attcggggta
     2341 aggaatactt cctttcggtc cggtattaca taattacggt cgagtcgatg ttcgtaaaaa
     2401 tatttccttt taaatcttcc tgtgacatag ttgtgataac cgtacggttt ttcacactta
     2461 ccacaaaagc agtcctgtca acgagtacaa gtgtttcgtt gctgcccttt tccgcggcta
     2521 tctcaatgcc gtcgttaagt ctttgcagca tatccgagtc aagtatctcg atatttctgc
     2581 tttctatacg cttcattgcg tgttttgaaa agcctaccgc cgagttcctc gcatcaagct
     2641 cactttgtaa agcctctgca aatgtttctc ccgactgctg tgcggccttt tcagcattcc
     2701 ctgcttttgc ggtgttgacg ttgcctgtcg aaatctgtag atttcgcagt ttcagttcat
     2761 ttatcaacat aacactgccg ccttttatta tctgttgccc tccgcattgc ttgccggagc
     2821 acctgttttg ttgtcagccg tacttcccgt accctcgctg acgtgtacta tctcgtcaat
     2881 gnnnnnnnnn nagataattg tcaatcacga tcttcgcgtc gccgtcctgc atctccgcag
     2941 actcgacctt gccctttgcg taatagaatt tgccgtcgtc gccaagcacc tttaccgtta
     3001 cttccttgcc cacatatccc agtgcctcgg tcattgtatg taacgtaccc ttgcttaccg
     3061 tatcgcttat cgcctttacc gccgataggt cgtactggtt gccgtcaact actaccttta
     3121 catcacttcc gctgatgttc actccggtgc atacgcccga taaaagcgtt ccgtcatcac
     3181 tctgtgcatt cattgtaagc gttttaccca caagtcctgc cgcatagttc gacatactgt
     3241 acttcagatt atcctgctgt gcctgtaatg ccgtgaactg tgccatctgc gacacgaact
     3301 cggtatttga tgtaggctca agcggatcct gattgctcat ttccgccatg agcaggttga
     3361 agaacgtatc cgtgcttatc gtatcattct tgcccgaatt tttgaaaaga tccttgtact
     3421 tttcgtgatt tgactgtaca ctctgaagac ttgaaatgtc tgccatctgt aatcctttca
     3481 tgtcagtccg actgataata catcagtcca ttgcctgtat ctcgcccaac agactgtcaa
     3541 agtcgtctgt gtcgtctttc tttcgatcgt tcgaccgctg tctgtaaggg ttcttgctgc
     3601 tgccgtcaaa atctctttgg aataattccg tctgcgaggt ttctaccttg tacgaggtaa
     3661 gctctacatt gtcatttctc aatgcatatg ccagctgttc ggacttcgcc tgtaaaagct
     3721 cctttgccgc ctgagtttcg gtaagtatct cgaccgtcag ctttccgctt tcgttcacca
     3781 tacggaccgt tatctttcca agcgtttcag gtgtaagcan nnnnnnnnnc ctgccggtac
     3841 tcttaggtgc gcgttcgctc acaaagcttg ccatatcgct tatcggcact cttaccgtct
     3901 gcgtaggctg accgttatcg tcaaagcgac tctcggttct tgcaaacaca tccgcacttc
     3961 ttgcactcat atcgcttacc gcattcttat cggtgacctt ctgcactgcg tcaagatcgc
     4021 tgtccgactt tgtaacctcg tttcccttgg ttatcgcctt aagctcttcg cttttgacct
     4081 cgatatttcg ctttgcataa gcaactctcg gtatattgtc cgcttgagcc gcggcttcct
     4141 gctttgcttc gcccgttaca ttgccggatg ttttctcaaa agtcatcttt acgatatccc
     4201 tctgaggttc tgcatctgtt tcagcgttgt aggtttttcc cgccgcgctt agatccacag
     4261 ccttgctttt gccgtccgct gcgacattgc cgtacctgtc gactgccgca ataccctccg
     4321 gctcgctctc aactgcgaca tctcccggat tactcatatc cgcgatatat acggactcac
     4381 ccgtgacagc attttcttcg gtttttccga tagcggtatg atctgccact gtgcttgcat
     4441 cgcctgctga accgacctgt cggtaagctg tagtcattat atcggtttcg gtaaaatgac
     4501 cgtttcttac agtttcttcc gttgccgtac cgatatctct gttccgaata ccttcgtcag
     4561 agatatatcc gatatcaatt gttccgcggc tcgaaacacc tccggataca ccgactgttt
     4621 cattaccgat atctgtattt tccgcaaata tcattcgcgc ggtttcaaca aagtcacttt
     4681 cgggagcttt gccttccata atttcactta actgtgaaat ctgcgccgag cttccaccga
     4741 cctgccgaac atcagtccga tctgtaccgt tttgtaccac agtctgtacc accgactgca
     4801 tactctcatt gtcggcagat acccgcataa actctgcgtt atccgctgcc ggagcatcat
     4861 tcgccgtctg taccactgcc gcctgcatat atcctgcaag ctccgctgtc ttcaacagct
     4921 gcttcagttc ctctgcgctt tgctcaagct ctgtaagccc gctgagtatc tcgccgggca
     4981 tagcagtctg tccttccgtg gctggtgtat ctgctgattt tccggcgctc tgccgactgc
     5041 ccgataccat agcccccagc atttccgaga agcctccggg cgcactgtta tcagcaattg
     5101 cagcatctga gattgtggcg gcattgcccg caaatgccat ggcggcacct gccggcaata
     5161 ctgctgttgc attcatcata tctcaccccc tttcagtgct attcatcatc gtaacaaatg
     5221 tatccgttac aacaactttt atatatttaa cagcatcaaa ccgttaaatt ttatgtaaat
     5281 tgcacagctt tccaccttgt attgtaccac atttcaagcg atatgtcaac aatttactcc
     5341 gttttactgc catttgaaga gccgttcgac acgaactcct caatgatctg ctcctgctcc
     5401 ttctgagcgg catatctgta ctcttcaagc tgtttttcct ccagcttttc aagctttgac
     5461 acttcctttg tagcgtcaat taccctctgt aattgctgtt ctacctcatc gcgcttttga
     5521 gccatacgtc cttttatgcg gtagatgtcc tgctgtagcg aggaaacata cttcttatgc
     5581 agtgacagcc gcattgccgt tgtcccgccc cgcacaagct cattgagctt gtctatgctg
     5641 agctcgtatt ctgcgttctt cgcgtcaagc tccgcctgca tttccgcaag ctccgctctc
     5701 agtctgccga gcaccgattt ctcgctgtcg agcatctgat cattgtactt tttcagcgac
     5761 tgcaacgtaa aattaaactt cttcataacg cactgttatc acgattttca cgacaccgcc
     5821 gccttgagca gctttatcgt atcctccatc gagaacgatt catacattcc ctgcataagg
     5881 aagttgttga ccttgtctat cttctttatc gcctcatcca gagcgggatt agttcccttt
     5941 ttgtaagctc cgatcgagat gaggtcggca ttgttattat ataaagccag caggtttctc
     6001 agcttgcccg ccgctttctt gtgctcagga tcggcaatca ccgacataag tcgtgatatg
     6061 ctcggcaata tgtcaatagc agggtagtgg ttctgcgccg ctatcttacg actgagaacg
     6121 atatgtccgt caatgatacc tcttacggta tcggatatag gctcgttcgt gtcatcgccc
     6181 tcaacgagca ctgcatatat acccgtgata cttccttttt caaagttgcc ggctctttcg
     6241 agcagcttcg gcattgcggc atatatagac ggggtgtagc ctcttgctac gggcggctcg
     6301 cctgtagata agcctatctc acgctgtgcc atcgcaaaac gcgtcaggtt atccatcatc
     6361 agcagtacat ccttgccctg tgcgcagaag tactcggcta ttgccgttgc cgtcatcgga
     6421 cacttattcc tcatcattgc cggctgatcc gacgtggcca ctaccacgac tgaccttgcc
     6481 attccctctt cgccgaggtc attctctata aactctctta cctctctgcc tcgctcgcct
     6541 acaagcgcta taacattgag gtcagccttt acctttcgtg cgatcatacc cataagcgtt
     6601 gatttgccga cacctgagcc cgcaaagata cccattctct gacccttacc cnnnnnnnnn
     6661 nggtgagagg gtttaccggt atgcccgcta taggtactgc atctgtatat tccacagggt
     6721 caccgccgtc aagcggattt cccagtgcat ctattattct tcctacaagc ttatcgccga
     6781 cccttacatt cagcttatcg cctgtattgt ccactatact gcccggacct ataccttcaa
     6841 gatcggtgta gggcataaga agcactttgt gctcgttaaa accgaccacc tcggcaggga
     6901 tatgcgactt catatccttt gtatagatac gacatacatc gcctatgctg caaagcgggc
     6961 cgcttgcctc aatcgtcatt ccgactatct tttcaatttt acccagatac ttatatgtat
     7021 cggtagtcct gagttctgtc agaaatccgc cgagatccat gtctaaagtc ctttcaataa
     7081 gggtgattat tcgcttgtct gttcagctcc gtcactgtcc ggaacggctt cggcagtttg
     7141 ctcttcctgt gccttagcct tttttcttga cggtgctctg cggtactttc ctctgccgag
     7201 ctcttctatc atcttgagct gtgtctgtat tcccgcatcg gttatctcac tgccgttatc
     7261 tataataaca gtgccgccgg gtgcatcttt aagcacctcg acttctacgt tttttacatt
     7321 ggtgaacagc tttttaaaat agtcggtatc cgccgccatt tcctcgctga catcggtctt
     7381 tgataatgtc agcttgacat attcgcttcc gcgaaagctc ttacccgctt ccttgatagt
     7441 cttcctgaca accgctttat ccttctgacc cagagagttc agcgttatgc ggttgactat
     7501 ctccataacc gtatcgaata tctcgacctc atagctgtcg aaaagctccg ccttgtcgct
     7561 taccatctgt tcgctgagct ttttaagctc tgtaagcata tcgcggcatt tatcaagtcc
     7621 tgcggcatag ccgtcctcgt gaccctttgc aaagccgtct gccttgctct tctcaaaaat
     7681 agcctccgcc tgctcctttg cctttgcggc gtaatcctgc gcctgctttt ctgtatcgga
     7741 gagtatcttc tgtgcacgtt cacgggcatc ggcaagcgcc ttttcgccct ctgcacggta
     7801 atgctgtttt accttttcaa tttcttcgtc tataagccgg ttgcgctgta atgccgcttc
     7861 atcgaccttt tcggtacttt ccgcagtttc agtgatctct tgtgtgtcat ctgcgatgtc
     7921 cggcggcgcg acaggcttaa tctttattga attgtcgatt acgacagtat cggttgtcgg
     7981 ctgaacaggg tagtgataac ctcttcctgc cttgaatacg cctgccaaaa tatattcctc
     8041 gctttcgtct tatgcgatga tctcatcttc cttgccaccc ttggagataa caagcgttcc
     8101 ctcatcttcc agtctgcgta tgatagatac aattctctgc tgagcctctt caacatcacg
     8161 catacggaca ttatgcagat actcaacgtc tgtctgtacg ctttcacgca tacgagtgga
     8221 catatttgca tagatgcact ccgcaacctc gggtgtcgaa cccttgatgg caacggcaag
     8281 atcctttgag tcgacctcgg ggataaatgc ctgtatagcc atattgtcga gtgtaacgat
     8341 atcctcgaat acgaacatct ttgaacgtat atcgtccgca agcttcggat ctctcagtgt
     8401 cagttcatca aatatatact tttctgtagc gcggtcgaca ttgttcatga cctctgctac
     8461 ataatcgata cctccgacct ctgtactgtc ggctgtaaca aggttatcaa atttcttttc
     8521 aagcgtacgc tctatagact tgacgacatc gggcgatgct ctgtccatct ttgccatacg
     8581 ctcaacgaca tctatccttg tcttcttcgg cagttcgctt aaaatggcag atgcctgatc
     8641 gctccttgcg taggatagta tcagcgctat agtctgcgga tgctcgttct ggaccatggc
     8701 aagcaggttt ttatagtccg ccttgcgtac gaacgcaaac gcttttgtct ggaattgctt
     8761 tgttatcttt tcaaacagag cctgtgcagt ctgcggtcca aacgcctttt caagcacatc
     8821 gcgcgcatac tccacaccgc cctcggaaat taccttctgg gtaaggcaaa gctgaggctc
     8881 aaagctgagt gccgcagatt cctgcgccgc ctgtgccgcg gctgcctgtg cggcaattgc
     8941 ttttgctttt gctcttgccc gctttttctt ctttgcattg cttgtgagta tagctacgat
     9001 aagaagcgcc actacaagaa gtcccagcag gattataata tataccgtcc agtcaacgtt
     9061 tccgcctctg tttatggtat tgccgttacc gttagcgccg ttgttgccgt ttataacgaa
     9121 cggataatta gctaccgtta cattcgctac atctgcgccg atcgcgaatg ctatcacctg
     9181 acggagctgt tccttgtcag cctcaagcat attcacctga tcgactgtca cagccgcagt
     9241 tatcttannn nnnnnnntat cgaccgagta gccgttcttt tcaagctgtc ttatctcagt
     9301 gtttacaaga cgttttacgg taatgctgtt ctgatagtaa tattcgttgt caccgtcacc
     9361 cttgatggta gggtagttcg gtgaaatatc gctgtttctt gtagtgccga ccacgcccga
     9421 cagtgaacct ataccgcccc agccctcatt cttggtggag ctctctatca taccgcccga
     9481 tgtgccatcc tcggcgatcg acggagtgta gattgtatat tccgactttg aattgtcgta
     9541 attcagcgtt acatttacgt ttatcgtata gtccttgtac agctttttca gtgtgtcgtt
     9601 gagctgactt ttcagcaggt tctgcatctg ctcctggaag ttcagccttg actgatacag
     9661 tacgtcctct tcgaccgttg tattttcgcc tgtcagaagc ttgccgtccg tatcaagtat
     9721 cgagatattg tcacgctcaa gtcccggcag ggaattgagt accaatgcat aaatgccctc
     9781 tacctgatcg cttgtaagct gtgcgtcctt cttcaggtca agctttacgc tgactctcgg
     9841 gtcttcactg tctgtcgaga taacatagtt gttgttctcg ggcattgtta ttataacata
     9901 tgagttcttg accgcgctta tggtatttat cgccttcata aggtgcgtct gcagctgctg
     9961 tatctggagc accttttttt cggtatcggt cgaccatata cctattccgt tgttccatac
    10021 gtcaaagttg aacgttgact taggataacc ctgaatactc agctgcattc ttacattatc
    10081 cgcctgatcg ctcggcacag aaatactgcc gtccgtcccc atcttgacat ctgttatccc
    10141 caactcattt atagcggaaa cgacctgcga gctctcttcg ctgtcaagtc ccgtataaat
    10201 agccgtgtag cctttctggt taagtactat cgcaagtacg atcgcggcaa ctacgactac
    10261 cgatactgcc gttattatga atatccgcag atttttcgcg agcgagttcc atttgttttt
    10321 aaaaccgttc cagaatgcgg aaagcttgtc cttcatctat atatgtatcc ttccctgata
    10381 atcggattaa acagtcatac gcataatctc attgtatgcg tcaacagcct tgttcttgac
    10441 tgtgacaaga agatcaacgg cagttgccgc cttttcaaga tttacctgta tctgcgcaag
    10501 gtcatctaca tcgcctagca taagatctat agaatcctgc tggacctgcg catcggtttc
    10561 cttgacatta tttacaaggg attcaaaaat atcgagaaaa gttgcatcgc cttctgtgct
    10621 gtcaccggtc tgctttttct gacctgcagc ttccattggg gtgatattgc ttgtaagcgg
    10681 tactataaac atctatatca gatcctttcg gttgtcttag ttatttccta tgctgagagc
    10741 ggtctgcgcc attgatttta cggcgttgag caccgcaagg ttagcctcat atgagcgggt
    10801 cgccgccata gcgtccatct gctcctccgc cacatcaacg ttcggcagat agtaatatcc
    10861 gttctcgtct gcatcggggt gggtcggatc atataccggt gtaagcggtg tttcgtcctc
    10921 gactatctcg gttacgagta cgcctttgtt tttggtcgcc gttaccgtat ttgcgggaac
    10981 accgcccgcg atcttctctt ttctctttgc tatcgtttcc tcaagcaccg agttgaagcc
    11041 cttgttttca ctgaacagca ctacctgtct tttgtaaggt gttccgtcct ctgttctggt
    11101 ggtttttgcg ttcgcaacat tctgcgatat tacatcaaga cgcagtcttt cggcagccat
    11161 acccgatgcg gctatattaa gcgagcttaa aaacgccatg caaaaaactc ctttctgtat
    11221 ctcaaaaaag cgtcgaaatc cgtttcactg tatataatcc attatattaa agcgtatgcc
    11281 ttaaatt
//
LOCUS       DAWAOG010000008        11206 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_993_length_11206_cov_2.157564, whole genome shotgun sequence.
ACCESSION   DAWAOG010000008 DAWAOG010000000
VERSION     DAWAOG010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 11206)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11206)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11206
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_993_length_11206_cov_2.157564"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1315..1377
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2386..2395
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3276..3285
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4484..4493
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5917..5926
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10626..10635
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3266 a         2223 c         2804 g         2800 t
ORIGIN      
        1 taagtcgata acattaccat tatcatcata tatcactttt gaaacaatga cactgtgatg
       61 agcccagcca tcaacgacgt actggataac atcaccgggc tgtgcgctga atatgttgcc
      121 gtcagtttcg gcaaccaagc ctttacctgt gttcttgttg cagtattcgt agaaatattc
      181 tgtccctgac catgagcccg acctgcctgt aggcgactcg gaaaggtcac cttcgtcatc
      241 aaaccatttc cattgctcgt ttccgctcca ttccatcggt attcccgccg cgtaaagcga
      301 ttgagaaaca taattctggc agttgccgcc gtagatcgca tagtcgctgt aatcggggtt
      361 tcttatgact ttttcaccgt cgacccattt atacgaatat gcgaccgccc tatcacggtc
      421 atattcgtgc tttgcggttt ttgaggtttt gaagctgtcg gggtctgcgt tatagtccgc
      481 cagcatcttc tcctgcccgg ttatgtcggt tccggcatcg gctttaagcg attttaatat
      541 gcctgcgtac cttttatcac aatcgagggt gtcggggatc ttcatatcct ttaaataata
      601 ctcttcatag tcatatccga ggtagtccat aacacgcttt tctataagca gattaacatc
      661 ggtatcctcc gcgagaatgt cgaatttgta tttgccgtct gtgcctttgg agatctgtgc
      721 ttcgacctcc attccgcagg actttgccgg ggtatcgcat attgcaaacg aaaccttttc
      781 gcttatggta tagtttatcg tgtaaatgcc cttttttacg gtcgcacttt ctacgtttat
      841 cacaaacgat gcttcatcat agcccagatc ggcagagcga cactgccttg catatatcag
      901 atattgcagt gaggtatcgc aaaagcctgc atacagcttg ccgtacgttt tgtcggcgtt
      961 gaaatacctg gagagcgact tgtattcaag ctcgcctgta tattcgtact tgtccgcagt
     1021 gtagtcaata agcagctgtt ttatctcatc gggaagcttt accttgtcct ctgtggtcac
     1081 ctcgccgctg acaagcgtta tcttttcggg ctcggctgtg tccgtgaccg gtaacgtttc
     1141 agcgtcagaa gatgtttctt cctgctcttg tgtgatgccg atatccgaag aggtatgctc
     1201 ctcgttgctg tgctttacaa aaccgagcag tgacagaaat gcgcctatta cgatcgccgc
     1261 cgcaatgcct atcatggcaa gccttttcag tttgggattt ctgtgcctgt atcgnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntac
     1381 aatgttattc tcctgttttt tctttcatca tatctattat atcgtttatt acggcggttc
     1441 gcaaggcgca atacgccgtt taccgactat tatgatatat ctccaacctt aaacagacct
     1501 tagttgacgg catttttgat ttgtcaaagt gcataggtat aaattgcgtt ttgatgaact
     1561 atgcacaaaa agtacgatag ttaattgact ttgatatgca attgtgataa aatcaatagt
     1621 ataaacagca gaataacgtt ttgctgtcgt aaaattgcat attgcataaa acaggagcag
     1681 acaaatgaat tactggaaca aagaaatcga atgtgcgccg agaagcgaga tagaggcgtt
     1741 acaatcctac agactttcac gcacaataag acgtgtatat gaaaacgttg cgccttaccg
     1801 caagaagatg gacgaggcgg gtgtaagacc tgaggatatc aagtctattg ctgacctgtc
     1861 aaagcttccg ttcacgacaa agcaggatct cagaaacaac tatccctacg gtatgtttgc
     1921 cgtaccgagc tccgaggtcg taagaataca cgcttcatcg ggtacaacag gtaagcagac
     1981 ggttgtcggc tatacggctc acgatattga cgtgtgggct gaatgtgccg caagagcact
     2041 taccaatatc ggaggaacaa aggacgatta cgttcaggtc tgctatggtt acggattgtt
     2101 cacaggcggt cttggtatgc actacggtac agaaaagctc ggtgctaccg ttattcccgc
     2161 atcggcaggc aacagcttac gtcagcttga aatattcagg gacttcggca cttctataat
     2221 ctgtatgacc ccttcatacg ctataagcct tatagagctt atgcacgaaa agggcttcaa
     2281 gaaggaggac ttcaatctta aggcaggtct gttcggtgct gagccgtgga cagacgagat
     2341 gagaaagcag attgagaacg gactcggaat caatgcctac gacatnnnnn nnnnntcctg
     2401 cgagtgcgaa tgccagtgcg gtatgcacat ctgcgaggat catttcatag cagaggtaat
     2461 cgaccccgat acgcttgagg tattacctgc aggtcagcag ggcgagcttg tattcacctg
     2521 tatcacaaag gaagcgttac cgctgataag atacaggaca agggatgtag ctacgcttat
     2581 ctatgacaag tgtgagtgcg gcagaactca tgttagaatg ctgaaacccc agggcagaac
     2641 tgatgatatg cttatcatca gaggtgtcaa cgtattcccg tcacagatcg aatcggctct
     2701 gctctcggtt gacgaaaagg caaccaacta cctgttggtc gttgacagag tgaataatct
     2761 tgataccctt gaaattcagc ttgaaacgcg tcccgatatg ttcggtgacg gtgtaaagac
     2821 actcgaaaat tacacgagaa gggtaaaaac ggcgatagat tccgctatcg gcgtaggaat
     2881 caatgtaaag ctgcttgaac cgaagaccct tgcgcgctca atgggcaagg ctgtaagagt
     2941 tcaggataac agggctataa agaataagtt tgaaaagaaa taatcagagg aggtacagta
     3001 tgtatattga tcagatctcg gtttttgtag aaaacaagac aggcagactc gcaggtatta
     3061 tggaggtgct caataaggcg ggtatcgaca taagggcgat gactatcgca gatacggctg
     3121 atttcggcat tctgcgtatg atagttgacg atactgacaa ggctatcgaa gcacttaagg
     3181 ctgacggctg tacagcggct gttacaaagg tcatcgcatt tactatcccg gatgtttcgg
     3241 gcgcattata taacgttatg ggcaagatac acgacnnnnn nnnnncacga ctgcggaata
     3301 aacgttgaat atatgtactc ggtaatgggt aaggtagcag gcagagcgga tatcgttata
     3361 cgcgttgaag atgttgaaaa ggcatcaaag gtgcttatcg acaacggcgt gacgcttatc
     3421 gaaaagaagg atctctgcaa gtaagcggta agcagaatat tttccgctcc ggtgcgtatt
     3481 gcccggggcg gatttttata tgaaaagggg taaaatatga gcgaatatat tattaaacac
     3541 aacgagctta cagcggagca atttttgtat ctgtgggaaa gcgtatgggg agaaggaccg
     3601 tcacctgagc agacacggtt cgctatggag catacactgt ttcgtgtttc ggtatttgac
     3661 agtgggaaga taataggaat ggcgagaatg ataggagata tggggcttga ttattacatc
     3721 aaggacgttg ttgtgatacc cggatatcaa ggcagaggta tcggacgtat gatgataaat
     3781 gagttgcttg attttataaa gcacaacgga gtaagcggca cgggtatatt tgttgagctg
     3841 tgcgccgctc ccgataaagt accgttttat gaaaagcttg gatttgatca taatgacgca
     3901 atacgaatga agatgatgtg tgtggcagag aaataggctt gtgtaaacgt gcgggaaaaa
     3961 ctgtcatata aattttacat ttccgctatt gacaaaaaca tatagctgtg ctatactcaa
     4021 accataggaa atggtggtta ccgctgacgg ataaccgtga atcaacacgg gtgcggtaat
     4081 agcctgcccg cctgatcaag cggacacctt atgccgaccg tctgggcagt ccgaataggg
     4141 ctgcattttg ttttgtaacg gaaaaatatt ccgctgaaaa gcagcaaacg tgcattgcgg
     4201 tcaatccgca ggaaggaaag gtaaaactat ggaaaacaca accaatctca gggttcgctt
     4261 caaggagtac tgggacggct tcaagcccgg tgaatggcag gagggtatca atacccgcga
     4321 ctttataatg acaaacttca cgccgtactc aggcggaccg gagttcttat gcggtcctac
     4381 cgagcgcaca aaagcgctta atgcaaagct tggcgagctt ttcaaaaaag agcgtgaaaa
     4441 cggcggtgta ctggatatta acactgaggt ggtatcatcg cttnnnnnnn nnntcgttgg
     4501 ctatcagacg gactctccgc ttcgcagagg tgtaaatcct ttcggcggta tcagaatggc
     4561 gagaggtgcg tgtgaggctt acggctacaa gctgtcgcag agcgttgaag aacactttga
     4621 gtatcgtaca acacacaatg acggcgtgtt cagagtatac acagatgaga tgaagcttgc
     4681 acgtcatata ggcataatca caggtctgcc cgacgcatac ggcagaggaa gaataatcgg
     4741 cgactaccgc agggtagcgc tttacggcgt tgactatctc atagagcaga aacagcttga
     4801 caagaaaaag gtcggcgaga atataatgga cgttgatact atccgtcttt ccgaggagct
     4861 tttccagcag atcagcttcc ttaagaagat gaaggaaatg gcggctatgt acggctatga
     4921 catcagtatg cccgcaaagg atacaagaga ggctatacag tggacatact ttgcttatct
     4981 tgcgtcaatc aaggagcaga acggtgcggc aatgtcgctc ggcagaacat cgaccttctt
     5041 tgatatctat gtcaatcgcg atatggaaag aggtatcctg acggaggaac aggcacagga
     5101 gcttatagac gactttgtaa tgaagctgcg cagtgcaaga catctgcgta cgcccgaata
     5161 caatgagctt ttcggcggcg acccgatgtg gataacagag agtgtcggcg gtgtgaacaa
     5221 cagcggcgtt ccgctggtta caaagggcag ctacagaatg ctgaacaccc tttataacct
     5281 cggttcttca cctgagccta accttactat actttggtcg gaaagactgc ccgaaccctt
     5341 caagaagttc tgcgcaaagc tgtctatcga caccgattcg atacagtatg agaatgacga
     5401 cctgatgcgt atggaatacg gcgatgatta tgctatcgcc tgctgtgtat cggctatgaa
     5461 ggtcggcaag cagatgcagt tcttcggtgc aagatttaac ctgccaaagc tcctgcttct
     5521 tgcgataaac ggcggctatg acaatgtaac cggtatgaag ataggtccgc agatggagcc
     5581 tttacagggc gacaagctcg attattatga ggtaagaggc agacttgacg tttaccgtga
     5641 gtggctgtgc aagctgtatg ttaatacgat gaacgttatc cactatatgc acgacaagta
     5701 cgcttacgaa aagacgcaga tggctctgca cgatacggac gttgacagaa tgatggcgtt
     5761 cggtatagcg ggactgtcgg taatggctga ctcgctgagc gctataaagt atgccgatgt
     5821 aaagcctatc cgcgatgaaa acggctatat aatcgacttt gatacaaagg gcgacttccc
     5881 gaagttcggc aatgacgaca acagagttga caagatnnnn nnnnnntact atcacatcaa
     5941 acgttgtgta cggcaagaag acaggctcaa ctcccgatgg aagaaagaag ggcgagcctt
     6001 ttgcacccgg cgcaaatccc atgcacaacc gtgaaacgaa cggtgctata gcatcactga
     6061 actctgtttc aaagcttcag tacgactact gccgtgacgg tatttcaaat acattctcaa
     6121 tagttcccga tgcgctcgga aagaccgagg agcagagaac ggcaaacctt gtgaccgtgc
     6181 ttgacggcta cttctcgaat tacgcacatc acctcaacgt aaatgttctt aataaggaaa
     6241 cgcttattga ggcttatgaa aaccccgagg cttatcccaa tcttactatc cgtgtatcag
     6301 gctacgctgt caacttccac aagctgacaa aggaacagca gagagaggtc atcagcagaa
     6361 cgttccacac ggtgatgtaa tatggagtgc agatacagtg caatacagac gctcggcacg
     6421 cttgacggcc cgagggtgcg gtttgtattg ttcctgcagg ggtgtccgct ccgctgcgga
     6481 tactgtcaca atcccgaaac aagagatgca agcggcggaa aaacagccac cgtcaaagat
     6541 gttatgcaaa gggtgctgag atgccgtaat tatttcggta aggacggcgg tatcactgta
     6601 tcgggcggag aaccgcttat gcaggcgaaa ttcgtaacag agctttttaa ggaatgtaaa
     6661 aggcagggaa taaatacctg ccttgacaca tcgggctgta ttatgaatga cgatgtaaca
     6721 gagcttttaa aagtgaccga cctttgtatg cttgacatca agatgacaaa cgatgaggat
     6781 tacagaaagc atatcggctg ctcccttgac gcaccgttga aatttctcga caagcttgcg
     6841 gaaatgaatg ttgaaacgtg gataagacag gtgacggttt gtggagtaaa tgacgatgat
     6901 acgaatatca aaaggcttaa cgatatagca aacaggtatg ataatgttac ttttgcggag
     6961 cttctcccgt tcaggaagct gtgcaagtca aaatatgacg atatgggcat tgagttcccg
     7021 tttgactgct acccggaaac ggggcaggag gtcatagaga ggctgaagcc ttcggtcgat
     7081 aagtttatat tctgacagat acccatcttt cacgagaatg aaggatgggt attttgaata
     7141 ttttttacag agatccgatt tttgttgact tttctgaaac aatatagtaa aattaaataa
     7201 gcgtgtataa tcatttccga tatacgattt aagcgagggg ggactgtgaa tatgagtaaa
     7261 accgttttat ttgtagcatc ggtaacaaaa aagcatattt tgcagttcca tctgccgtat
     7321 atgaagtggt tcaaggatca cggatacaat gtacacgttt gcgccggtga tgattttgaa
     7381 ccggatgaac caaagaatat accatattgt gataaatact atattatccc gttctaccgt
     7441 tcgccctttg caacgcagaa tttccgatct tacagagagc tcagaaagct gatagacgaa
     7501 aacgagtatg agcttgtgca ttgccatact cctgttgcgg cggcgatagc gcgctatgct
     7561 ttcaggacgg caagaaaaaa acgcggtaca actgtactct atactgcgca cggtttccat
     7621 ttctataaag gtgcgccaaa gataagcagg ctgtattaca ttgccgaaaa agcgctgata
     7681 aggtataccg acggaataat aacgataaac gaagaggact attcggcggc aaagaaaatg
     7741 tgccgcggca agaaatgtaa agcgttcaag atacccggaa taggcataga tctaaaccgt
     7801 atcagaaata ccataaggac aagggaagat atacgcgaag agttcgggat accgaacgat
     7861 gcctttctgg taatgtcaaa ctcggaaatc aatcaaaata agaatgttga gtgttctatt
     7921 accgctgtag ccgctaataa aggcgtgtat atgctgatat gcggtacggg aaagagcgcc
     7981 gaaaagtgca aggagcttgt aaaggagctg ggatgcggcg acaggatcat tttcgctgga
     8041 tacaggtatg atgctaagga gctgctgcat ggagcggacg cttttatatt cccgagccgt
     8101 cgtgagggac tgggacttgc ggcaatagag gctatggcta caggtctgcc gcttatcgta
     8161 tcggataaca gaggaacaag agagtatgcg gtaaacggag aaaatgcgat agtctgtgaa
     8221 tataacaata tatcacagtt tataaatgcg gtgcagcttt taagctctga cagggagctt
     8281 tgcaaaaagc tcggtcataa cggatatcat accgccgaaa gatacgggct tgataattcg
     8341 atagccgaaa tggcggcaat ataccgcggc tatcttcaaa ttccctcaga aaataaaacg
     8401 ggagtgttat cgggtgaaaa taagtaatta caaaaggata ctcggatatt atcccgggtt
     8461 gctttcggcg gcggaaaaag ccgcaaagaa aagtaaagaa aagttagcac aggtaacggc
     8521 atatgtcact gcacctgtgc tgttttgcta tgtaagctat gtcattgaaa acgctgaaaa
     8581 agagggtata aagaaaatat ttttcttggc gcgtgacgga tatatactca aaagcattgc
     8641 agatgtgatg ctaaaaagca aaaaatcaga tatagaaacg gattatcttt atgtttcgcg
     8701 ctatgcgctg agaaatgcga tgtattttaa atgtgatacg gtgcaggatt ttgagagcgc
     8761 gggttttttc ggacactgtg cggtgcagag tgcggagaat acacttaaaa gagcgggaat
     8821 tgatgacagt caacgtgtac gggtttacaa taaaatcggg tttgacggtg atgaaaaaag
     8881 aattatggac gatgcggagt atgatgattt ttgcaagctg ttaaaatcgg ataaagaatt
     8941 attttcggat attaaaaata attcgcggga gaaatacgaa aatattatcg ggtattttga
     9001 gcaaagcggt ctgtttgaaa ataaaagaat tgcgcttgtt gacagcgggt ggctcggctc
     9061 ggtgcagaga acgcttactg agcttattaa ggacaatacg gataccggca ggattacggg
     9121 atattatttt gggctttaca gaaaaaagag cgaacgctac aggtcttttt tgtttgatat
     9181 atcggacgct tatagatatg tgccgacgtt ctgcaacaat ctgttcgagt gcttctgctc
     9241 tgcgcctcac ggcatgactg tcggttatcg ccgcacggaa aacggcatac agcctgttac
     9301 ggcgaaagta aacgactata tagccgatgc cgcagaaata cagaacgata tcgctgtgag
     9361 atttgcggcg tatgcttgtg agagtgatgc ggtcattccc ggaaaaaagc gtgaaaggct
     9421 tacagcaagg ctcataaagg cgcttatgta taagccggat atggaggaag cggaaatcta
     9481 cggcgcgttt ccgttctgcg atgacgctac cgagatatct gtacagccgc ttgcgtgcga
     9541 cagcggaaag agcatacgca atattctttt tttgaacaga atatcacgaa aaagaaaagg
     9601 tcagaatata tatcccgaca agggtatgtt ctggctttac ggaagtataa gactgaccgg
     9661 atgtaagcct gcgtgggttt acagaagcag cgtcaggata tgggaaaaat taaggcttat
     9721 taaggaaaag aggaaacaat gccgctgata tcggttataa tgggcgtata caacagcgat
     9781 cccgtaaagc tcacggcggc tgtaaattcg gtactgaacc aatcggtcgg ggatatcgag
     9841 cttattatat ataatgacgg ctcggataac gtgaccggaa aaatactgaa aggatttacc
     9901 gaccgccgcg taaagatcat ctgttcgact gaaaataagg gtcttgcagt ggcactcaac
     9961 gcctgcattg atgcggcaga gggaaaatat atagcaagac aggacgatga cgatataagc
    10021 cttgaaggca ggtttgaaaa gcaagtgaaa tatcttgagg agcataagga tattgacttt
    10081 atcggtacgg cgtgtgagct ttattcggac agcgagggag tttacggtga gcgtataatg
    10141 cctgagcata tcgacaggga aagctttctg ttcaattcgc cgtttataca cggaagtatg
    10201 atgttcagac gggaagtttt ccgctgtcat cgctacagga cgctcggaaa gaaccgtaag
    10261 tatgaggatt acgacctgtt catgcgtctt tgcgcagacg gatacaaatc ggaaaatctc
    10321 actgacaagc tgtattgttt ttattatgat aaggcgttaa gagctgtttc attttctatg
    10381 cgttgtgatg agtataaagt gcgtatggaa tgtttcagaa tgctgggact tatgccaaag
    10441 gggattatat atgcgcttaa acctatcttg ctgggtttta ttccgcgcag ggcgactatg
    10501 aaaataaaag agaaaacaaa caaagtgtag cgtatttatg ttaatttact gacgcatttt
    10561 gcataactaa ccgttatatc gctttctttc tttggtgtat ttgcatattg taaagatgat
    10621 agaaannnnn nnnnnataga aatgtgttaa aatgaattat attttgtttg acagaataag
    10681 cgatataaca gcgaaagagg gttgaatata caattgagcg ataaattcag cattgatgat
    10741 atacttgccg aatttggcga agaaaagaac gaaaaaaatg cgcaaaagcc gcaggtcggc
    10801 agtaataaaa tacagtctgc aaagcaatca ccgggagcta ctatgatcct tcgttctatt
    10861 gacgaaaaga agagctcggc taaaagtaaa gccgatacca aagagatctt gactaaaagc
    10921 tctgcgaccg caaaagcaaa tacagttcag aacaatgcgg caaaaggtgc aagcactgaa
    10981 aaaagaaatg tttccgataa gcctgtttct tataaggggt tggacgaatt tgaagaaaag
    11041 aaaactgctg taaagagcaa atccgataag actgcggcgc ctaagaaaac ggcagctcag
    11101 tcgcacggta caggttcatc ggcagcaggc ggaaatatga agaaaagaag ctcggctgca
    11161 gatacagcag tcagaaagac ggaggtatca agacctgccg caaaga
//
LOCUS       DAWAOG010000009        10597 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1044_length_10597_cov_2.171315, whole genome shotgun sequence.
ACCESSION   DAWAOG010000009 DAWAOG010000000
VERSION     DAWAOG010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10597)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10597
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1044_length_10597_cov_2.171315"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2306..2315
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2960..2969
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2825 a         2350 c         2621 g         2781 t
ORIGIN      
        1 gtttcaaagc ctgccttcat taaggctgta acgccgccgc agagaacgca ctgttcgccg
       61 aagaggtctg tttctgtttc catcttgaat gttgtcataa gtacgccggc tcttgaacca
      121 ccgatacctg ccgcataagc aagacctgtg tcaagcgctc tgcctgtagc gtcctggtga
      181 actgcaacta agcagggaac gcctctgccg attgtgtact ctgaacgtac tgtatgaccc
      241 ggtcccttag gagcaaccat gataacgtca acatctttgg gaggtacgat ctgaccgaag
      301 tggatgttga agccgtgagc aaatgcgagt gtcttacctg ctgtaaggtt gggctcgatg
      361 ctctgcttgt aaaggtcagc ctgtctttca tcgttgatga ggatcatgat gatgtcagcc
      421 ttagctgctg cctctgccgc tgtgtatact tcaaaacctg cagcctcagc ctttgcccat
      481 gactttgagc cgttgtaaag accgatgatt accttaacgc cgctgtcctt taagttaaga
      541 gcgtgagcgt gtccctggct gccgtaaccg attattgcaa ctgtcttgcc atcgagcaga
      601 gaaaggttgc aatcttcctg atgataaagt tttgccattt taaagatctc ctttaaattt
      661 gttttcggag ttttgcctcc gtatattaaa gcgcggcttt tacagcttca tagaagcccc
      721 gcctttttgc attgctattg agcctgtacg gaccgtttcc tttattccgt acggacgtaa
      781 aagctcctca aacgtcttta tacggggaac gctgtcggat aattcaagcg tcatcgtatt
      841 taccgttata tcggaaattt ttgcttttgc tatctcggcg agcgaaataa gctctgccct
      901 ctgtgacggc tggcaagcta cctttataag tacgagctcc cttgataaaa gctcctctgc
      961 gggcaaatgg cgtaccttga taacgtctat cagcttgttg agctgttttt caatctgctc
     1021 taaggtataa gccgttccgt ctgcgataat tgttatacgg gagatttttt catcctcggt
     1081 aatacctacc gcaaggctgt ctatattgaa accgcgtctt gcgaaaagtc ccgcgaccct
     1141 tgcgagcaca ccggcgtggt tttctacaag tattgatata gtatgcttca taggctcacc
     1201 ctttacagta tagtcttttc gttttcggct actctgacct caagcagata acagcccttt
     1261 gacgagagca tctcgtcaat tgccttgtcc gcatcggcta tatcggtaac tctctgcgcg
     1321 tgtatgccgt agctgtctgc gagctttatg aagtcggggc ttccgtcaag gaataccgct
     1381 gtaagcctgt tgtcataacc gtttgtctga agctctctta ccatacctaa acgattgttc
     1441 gtcatgacgg ttacttttac cgcaatgtca tgctgtatca ttgttgcgag ctccatgaac
     1501 tgcatctgga agctaccgtc accgcatatc gcaatgacct cgctgtcggg gcacgctgcc
     1561 tttgcgccca gcgccgcagg aacggagtag cccattgttc ccatgccgcc cgatgtgata
     1621 aatctgccgt ttctgagtcc tatgttattg acagtccata tctggttctg tccgacatcg
     1681 gcgcttacca cggaattatc ggggagcttc tgaccgagaa gccttatgaa agccttagga
     1741 tttacacagc cctcgggctc ggcttcgatc ggcttgtcct gtgttttctt tacctcgttt
     1801 agctcctgct tccagtcgta atggtcgtaa ttctttgcgc attcaagcag ctgctcaagt
     1861 atgttcttta catcacctac aacgggtatt gttgcggcaa ggtttttgcc tatctctgcc
     1921 gggtcaacgt ctatgtgtat taccgttgca ccctcaagtc tgcttattgc agtgactgct
     1981 ctgtcaccga ctctcgcacc gagaagtatc aacagatcgc agttgttgat ggcatagttc
     2041 gcactcttta caccgtgcat acctatcatt ccgtagaaca acggattgtc ggcaggaaca
     2101 ctgctcattc ccatcatagt atttacaaca ggaatatctg taagctgcat aaactgctca
     2161 agcagccttt cggcatttgc ggtaaagata ccgccgccgg cacagataag tggctttttg
     2221 ctcttttcaa tagcggcgac agcgcgcttt atctgcagct tgttgccctg aacactcggc
     2281 ttgtaagagc ggatatccac tgtttnnnnn nnnnngcata tcaataagta caggacccgg
     2341 tctgcccgaa cctgcaatgt aaaatgcatt tttgaaaata accggcattt cctcgggatt
     2401 ttttacaagg tagctatgct ttacgaaagg ctcggcagca cctgtaatat ccgcctcctg
     2461 gaaaacatcc cgtcctatct gttcacagct tccctgccct gtgataagga ccataggtat
     2521 gctgtccatg tgagcggttg ctatagccgt tataaggttt gtcgcacccg gacccgatgt
     2581 agctattgcg actgcgggtt ttcttgtcat acgcgcataa ccgcttgcgg cgtgtccgcc
     2641 gttgacctcg tggcgcacaa gtatatggcg tatacctgag cttataagct catcataaaa
     2701 cggacatata gccgcacccg gataaccgaa tactgttgtt ataccctccg cctcgagagc
     2761 ttttaccata gctcttgcaa ccgtcattct ttctgacgcc atctatcgac ctaccccttt
     2821 gttaatctgt ttgtctttat accatatttt catatagcaa gccgcacggt aaaattcgtg
     2881 cttatcaata attataaccg ctttacaaaa aaaagtcaag atgagtttgc atataaatag
     2941 ctgtctgccg ggctgttttn nnnnnnnnng aacatctgtg cggtcaggtg atgactgaca
     3001 gcaacggaac gacttatata atatccgata cattttccgt gatatatccc ggtgatgcac
     3061 accccgatgt atacgaatgg ggcgatgttt catcggtaag gatagacaag ggaaatatag
     3121 agataacggc gggaaagcag acctatcata taccggaccg tgcatttacg ggacgttcgc
     3181 agtttacggc ggctaagaca ataatacttt ccgaaatatc gggcagcgat attgagtgcg
     3241 atacgcccgt tgagatactt cccgataaac ggttcttctc aaattacgat ataccggact
     3301 ctgcgatatt tgcaaagggt gaatacaatc cgaaagaaat acgttcatca ctgctgtcgc
     3361 ttgcgatagg taaggtcggc aaattcctgt ggtgcatagc cctggcggcc ggagtgcttg
     3421 cggcggtatt atttcatatg cttataggct ttgccgagga taactggtgg tatctcggaa
     3481 tgggtatatt cttctgtgcc gtaggcgcag tggcagttgc gtatcttata atggtagcag
     3541 tgacgaagct gaaatattcg gctatgataa agagctgtac ggacagcgat gaaacggtca
     3601 catttgcgat atgtccggtc ggaatatcag cggcagaaga cagtgtatac agtccgcacg
     3661 aaattatccg tttcggaatg aacgacaact acatcgaaac ctcctccatg tttattgtga
     3721 caaggagaaa agtgccgctt gtatggatac cgaagtcgtt gtttgacagt agcgcgcttg
     3781 ataaaataga acagtacctt gcgctcggaa cgcaggataa atgagtgaaa ggggcgaagg
     3841 taaaaatatg agtgaaaaga tacctcaggt agtatgcgag catatcaata taaatgacag
     3901 cgaggttatg gttttctcgg taaaggacgg tcttgccaag ataggcatat gccgcataaa
     3961 ttccgaatac gacaagtatt gtctttattc ccacgatgct ccggttatgg tcataagcgg
     4021 cggtatgaat gatgacgatg cggataaggt catcagtgag tatggcagtg cctcaagcgg
     4081 ctctataata gcagagagca tagacggtgc atacgcaaga cttgcgaaag gcggctcacc
     4141 ggagtatgcg ctcgagccgt ttatgcgtct gctccctgat ggactgtatg ctgtgacgta
     4201 cgttgaaaca tatcccactg caggcgaaag tatgtttttc tggtccggtt acggcgtgtc
     4261 aaagcaattg cgcaacagca gcagaaacat aagtgtgata ggggataagg cgtacagcgc
     4321 accttttctt ataccgaccg aaaaaccgtc cgcatatgac ccgtcactta ttaaaaaggc
     4381 ttctgccgag gggctcagaa acggagatct gttcggtatc tcattgcatt tctccgggct
     4441 ttattcggta ttgctcaaag gtcactatgc ggcttgtgcg gcggcaatag agggcaagcc
     4501 gttctatacc gtgcagatac agccgctcaa tgagatctgg aagataaccg atgagaacgg
     4561 cgaaaagaaa accataggct tttcttctgc ttctttccgt gttccgtttg agtcgctgac
     4621 aaaagaacag ttgcgtatcg gacttacatc gagaaaatac gttacaccgg acacttatga
     4681 cgctgtaaag cagaagctcg gtgtagtgcc gaagtccgga cagagacgct tgccgaaaca
     4741 gctggattca ttctccgaac gctacccgga cgctgatatg gtggcgtgtg cacagggcgt
     4801 tgaagaactt acaaagccta tgcttgacgc attgctcgca ggtcagacag agcttgacgg
     4861 taaagtaata atttccgaga attattataa cagtgttaca gccgcgtgta actatctgcg
     4921 ttatcacaac aaggacgatt ttatcgattt cgctttgaat gttgtgcgta acccggagct
     4981 ttccgcaaca tatacctatg ttgcatcttg tcttgcaaag gtacacgatg acaggatctg
     5041 tcaatttttt acagaggtca aaaattcagg tgatgcggga tattcaaaga taataggaac
     5101 tgcccgttct tatatcgata attatgataa gcatgttgag gtgtcatcgg aggacttctt
     5161 caactctctt cccgaaccta aaaagggcag cgaacgcaag gagatcatac accttacggg
     5221 cgacaataaa acatccgctc agctcagcgg cgaaaccgca aaactgatgg cggcacattt
     5281 ggcttctatg atagatgcca agaaggagta attgcattcc gatacaaaag taaagggtac
     5341 tgagaatttt tcacagtacc cttatcagca tttttataat gtttatatcc gccgatggca
     5401 taattcatgc attgattatc gctttgttaa ttcccaccgt tttgctatcc tgagataagc
     5461 cgagttgtta taaaaggcgg aatttgcagc cgcaacggcg aatttgacgc ggctcacagc
     5521 cgtctgttta cttgaaataa cggtttaaaa gaccttcaaa tgccttgccg tgtcttgctt
     5581 cgtcctttgc catctcgtga acagtgtcat ggatagcgtc aagatttaac tgcttagctc
     5641 tctttgcaag gtcgagcttg ccctgtgtag caccgtgttc tgcggcaact ctcttttcga
     5701 ggttctcctt tgtcgatgcg cttacaacat cgcctaaaag ctctgcgaac tttgcagcgt
     5761 gttcagcctc ttcccatgca gccttttcat agtaaagacc tacctcggga tagccctctc
     5821 tgtgagctgc tcttgccata gcaagataca tgcctacctc tgtgcattca ccctcaaagt
     5881 tagcctttaa acctgcaaga atttcttcat cctcaactgc ctttgcaacg ccaaccttgt
     5941 gctcatctgc gaatacaagc ttctcgctct ccttgagctc tgtgaacttg ctgccgggaa
     6001 ccttgcactg ggggcagaac tcgggaggtg tatctccctc atgaacgtaa ccgcataccg
     6061 gacataccca ctttttcata gtaaattacc tatcctttca taaaataaaa tgatttgttt
     6121 cttgtcaatt ttaacaaaat cagtaacgtt tactgatttg atgatttaat tataaccgat
     6181 actttgacgt ttgtcaatag gtttaactaa attttttttg taaaaattag gttagcgtgg
     6241 gcttacttga ggcggtaacg cagaatagca cagaggagta tcttcaggcg agtatgatag
     6301 tgcagttttt tgaaaggctt ggtaaagcgc tgtcaccttt ggcaagggcg gcaagaatgc
     6361 ggtgcaggac aagggaaaac taccgtttca agccgattaa acgccgtttg tgccttttcg
     6421 ttgcaattgc cgatgaaaaa gattataata tgtgagaata tggttataat tccgattaag
     6481 agaaaaggag agtttatgag aaaaacgaac gaaaagatac tgcgtacatt gcttgcattt
     6541 tctgtacttg caacggcact tctttcaagc ggttgccttg acgatgagga agaggtttca
     6601 gacagcacgt caagtgataa taggcagaca agcgagagca aaaattcgga cggtggcgaa
     6661 agcggcggca ataagtatga cggcgaggtc aatggcagcc gcgcgtcaaa gctgatgttc
     6721 agcgatttgg acggaataag cattacaaga aaacagcgtg aaaccgaaaa gcctatgggc
     6781 gaggacggca cacagacggt attcatctat atgtgcggca gcgaccttga aagcgagaac
     6841 gggcttgcaa gcggcgacat tgaggaaatg gtcgcagggt cacaaagcga gaacgtgaag
     6901 tttgtcattc agacgggcgg cgcgggtgca tgggctgata catacgggat ctctgccgga
     6961 aagacacagc gttatgttgt aacgggcgga gaaataagcc ttatcgaaga aaaagagtct
     7021 gtcaatatgg gcaaggagga tgtgcttgtc gattttctca gctggggtat agaaaactat
     7081 gcggcggcta aaatgggggt tatcttctgg aaccacggcg gcggaagtat aagcggtgtg
     7141 tgctttgacg agctgaatga aaacgattcg ctgtcgcttg aggaaataga cacagcactt
     7201 acctcaatat acgataagat gacggataag tttgcgttca tcggctttga tgcttgcctt
     7261 atggcaacag tagaaactgc aaatatgctt gttcctcatg cggactatat gtttgcgtcc
     7321 gaggaaaccg aacccggtta cggctgggat tataccgaga tagcaggctt tatggaaagt
     7381 aatcctacag cggatacagc cgaactcgga aagacggtcg ctgacagctt tatggcatcg
     7441 tgcgaggcta tcggcgcagg cggtgaggcg actctgtcaa ttacggatct cagccgaata
     7501 gatgaacttg taaaagcggt aaatgatgcc gcttctgaaa tgaacgcagg ttcagccgat
     7561 tctgcaatgc ttgcagaaat gataagaagc atctatgggg taagatccta cggcagcaat
     7621 aatgaaaccg agggctatac caatatggtt gatctcggct ctatgataag cgcgacagtt
     7681 cccggtgaca aggcagacaa ggttatgtcg gcgctggaaa aggcagtcgt gtataatatc
     7741 tacggcgcgg acaagtacgg atctacaggc ctttcgacct actatccttt cggtgtttcc
     7801 ggctctgccg agcttgcaat attcggtaag atatgtgtaa gtccgtacta tatgaccttc
     7861 gttgacagaa tagcctacgg cgcacaaaat ggcagtacgg acggatacag cggtgaaaat
     7921 acatggctcg ctgacggtgc ggtgtactgg agcgacggtg attattccga ctatgaaagc
     7981 aactgggagc attacaataa tcagaacgcc aatatggatt tctgcggcga taagagcagt
     8041 gtgcagatag atgtcggacc tgtgcttgac gatgagggaa cattctattt caccgtaacg
     8101 gacgatacaa taaataatct tgcaagtgtc aattgctcgg tttaccgtgt ggacgatgaa
     8161 acaggcgaac tgcttgaatt tggtgttgac cagagtacaa aggtagattt ctcttcggga
     8221 cttgttgaag acactttcag cggtcagtgg tacatgatcg aggataacct cattcctatg
     8281 tatatagtag aagaaaaagg caacagctcg gtttacactt cacctataaa gctcaacggc
     8341 aaggaaacca acctgcgtat agctatggag cgggacggca atgcgtatga cataacggta
     8401 ctcggaacat gggacggtgt aaacgaaaac ggcgagtcgg caaggagtgt tgttccgctt
     8461 aaagagggcg atgtcatagt gccgatattc aacacctacg acagcgaggg taactttgca
     8521 ggcaaggcgg agggtgatga atgcacctat agcggtgaca atatgataga gtttgtcaat
     8581 ctgcctgcag gcgactacag atacagcttt gttataaacg atatttacgg aaacgtgtgc
     8641 tatacaggct tcacggtgtt tacgacagat gaaaacggcg atgtttactt caatccggag
     8701 gaataagcag gacagcaagc tgtatcgatc acgcattttt cttttacaaa aaaactaaga
     8761 ggagaacccc tatgccaaac cggatcatca cagatatttc agattatatc tttgtgtcag
     8821 gagaacctca aaaggtcgat gcgatttttc tgcctggcgg ctcacacccc gaacagcccg
     8881 aatatgccgc agaattatac cgacaaggtt tgactgattg gatagtcccg tcaggaggag
     8941 taagcgttaa agaggaaaaa tggcacggcg tccgctcaaa agcggaaatt tatagtggcg
     9001 attaccggtc agactgcgag ttttttacag atgtactcgt aaaaaacggc gttccgcaga
     9061 ctgcgatcat tgaagaaagc aagtcggggc atacccgtga taacgcattt ctctctcgta
     9121 aagccgttga tgaaaagggc ataatgataa aaagcggtat aattgtttgt aaagcctttc
     9181 atgcccgccg atgcttaatg ctgtatcaaa tggctttccc gaatgttgat atgacggtct
     9241 gccctgtaca ttgctataac attaccaaag aaaactggta tacgaccgaa aaaggaaccg
     9301 atcgagtgct cggtgagctt gcacggtgcg gaaatcagtt tgtcggcgat ctcaagactt
     9361 atcttgatct gatatgaaaa tactgtgctg ccttcagtaa aaacagcaag ccgtatcaag
     9421 tttcggtttt ctcgtttgtg ataaaatgaa tacagtcgaa aagatggaag aaagctgtgc
     9481 ggaggaaaac agatgaacgg gataaatgaa tggcttatga atattcagaa tatcgttgac
     9541 gacatcgaca gatgcatcaa aagcggcgag gacgagaaac tgacactggg caggctctcg
     9601 aagtcgctcg gatattcgga atattatgtt tcaagaaagt tcagcgagat atcgggaatg
     9661 aatctgcggg actatatgag ataccgcagg cttgcttttg cgctgaggga gctgagagat
     9721 accgacaagg gcatacttga tatagctctt gactacggct ttacatctca cgaggcgttc
     9781 acaagggcat tcaaagcggc atacggaatt actccgagcg cttaccgttc acaccctgtg
     9841 cctgtaattc tccgcacagc tataagacct ttcgactgct atcttttagg cataggagga
     9901 acaggtatgg cacagacaaa cagtgatatt aaggtgtatt tcgtaacgat cccggctcat
     9961 aaattcctgc atatcagaaa ctacgagagt atagggtatt tcgacttctg ggaaaagcag
    10021 agccgtattc ccggacagga ttgtgcaact atatgcggac tgcttgacag tatcaagggt
    10081 aagctcgacg atatgggcgg tgatgaggcg aacagcggaa gcggtcaggt catggcgtac
    10141 ataaacgagc ctgagggaag aatatgcagc tggggcattc cgctggctga ggcttacggt
    10201 gtaaggcttc ctgccgacta cagcggagag atgccaaagc agatgcagat catggacgtg
    10261 cctgagggcg agtatatcgt gtttgagcac ggaccgttcg actttcagac cgaaaatagt
    10321 gctgttgaag cgaaaatcga gcaggctatg aaagactttg actatgaaaa gtcgggctat
    10381 gagcttgaca aaacgcaggg cagagtgttc tatttctatc acgactgcaa gaggtactgg
    10441 aagtatataa gacctgtgag gaaaaccgta taaacgcaga accgcctttt aaacagggcg
    10501 gttcttcgga ttatgccggg cgatgcactt gacaccggct tgattttatg ttatattata
    10561 gatacaaaaa gagggaaacg acagacggtc gctccca
//
LOCUS       DAWAOG010000010        10360 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1066_length_10360_cov_2.609413, whole genome shotgun sequence.
ACCESSION   DAWAOG010000010 DAWAOG010000000
VERSION     DAWAOG010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10360)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1066_length_10360_cov_2.609413"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4503..4512
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8420..8429
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8853..8862
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2983 a         2312 c         2359 g         2676 t
ORIGIN      
        1 cgctttatga gtacaatgac ggtacatcga caggtgtata cggacttccc gtttcacaga
       61 cattcccgat gctgttctac agaacagacg tacttgagga gcttggcttt gaaaatccgc
      121 ccgaaacatg ggatgagctt accgatatgc tccccactct tcagagaaag taccttgatg
      181 taggtctgat acttcctcag aatatttctt caaatacatt cgattccggt aatacattca
      241 ttatgctgat gctccagacc ggacaggata tctacaatga agacctttat acaacagatt
      301 ataacagtat aaagacatct gatatcaagg gtctgaatct cacgaacttt atgactcagg
      361 actctataag agtgtttgag cagtggacaa agttctacac agtattcagc ttcgatcaga
      421 cattcgatgc gttctcacgt ttcagaaccg gtgagatgcc cctcgttgtt cagccgtata
      481 cattctacaa ccagctgtct gtagcagctc ccgagatcaa gggtctgtgg gacttcacgc
      541 tggtacccgg aacaaagcag gctgacggaa cgatcaacca cgctgcaaac tctgcaggaa
      601 gcggtgcggt aatattcaat aaggtttcca accaggctgc cgcttgggac ttcgtaaagt
      661 ggttcacaag cacggatata caggttgact acggtaagca gatagaagct cttatgggtc
      721 ctatgggacg ttttgataca gctaacgtag aggcacttga gcagctcccc tggtccacag
      781 cggagtacga gaagatctcg gctcagcaga gctaccttaa ggaagtaccg atcattcccg
      841 catcatatgc agttacaaga catataaaca acgcattcag aatggttgtt aacgatgccg
      901 gtaatcccag atatacgctt atgagttata atgatcagat aaagtctgag atcgtaagaa
      961 agtatcagga attgtcaagc gttaagaaat gatcttgaaa agccgtgagg gcttttgccc
     1021 tcacacgctt tgctgaatta tagaggaggt tatcggtttg tttaacaaag aaaaaacgca
     1081 gtatattgaa gacagcaaag tcagatatac actgcgagag atgaagaaga acggaagcgt
     1141 ttacgtactg ctcgctcctt acttcctact ctttacgatc tttacgatca taccggtgtt
     1201 gctgtcgttg cctatgggct ttacgaactt caacatggta cagtttcccc agttcgtggg
     1261 acttagcaat ttctattcgc tgatacttga ggacgaagtc ttcttaaagg cgatacagaa
     1321 cacacttgta tttgcgatta tcacaggtcc gttaagctat atcatgtgct ttatacttgc
     1381 atggcttatc aacgaaatgc ccagagcgtt aaagacgata ttcacattca tcttctatgc
     1441 gcctactctg gcaggtaaca tctatactac atggcagctc atcttctcag gtgatacata
     1501 cggtatagca aactcttatc tgcttgacct cggtatcatc aacagcgcac gtcagtggct
     1561 gacagatgct aactacatta tgggtgtagt tatcgtagtt cagctgtgga tgtcactggg
     1621 tgcgggcttc cttgctcttc gtgcaggtct tcagggtatt cctaaggaca gatacgaagc
     1681 aggtgctatc gaaggtatca agaaccgtac acagcagctt ctgctcgtta cggtacctgc
     1741 aatgggacct cagctgctgt tcgcagcggt tatgcagatc accgcttcct tcacagccgg
     1801 tgatgtaggt cgtttcctta ccgcattccc ttcaacagac tatgcggcac acacaattat
     1861 gacacacgca tatgactacg gttcaatacg ttatgagatg ggttattcat ctgcaatatg
     1921 tttcgtactg ttcgcagtaa tgcttcttgc caactggggc atcaaaaagc tcctcggcaa
     1981 gtatcttgac taatcggagg atcacattat gtatttttct ctaagaaagg aaggtagtta
     2041 tgaagataac taaaaggaga agaaaaagat atggcggttc tctcggcggc gacattttca
     2101 tatttgcagt gctcgttctg cttggtctgt tcatgctctt ccccatttat ctgtctgtca
     2161 tacagtcgtt aaagccggtc gaggaactgt tcctcttccc gcccagaatg tatgtacaga
     2221 acatcacgga tcagaacttt gctgatctgc ttgcaacggc atccaatatg tgggtaccgt
     2281 tctcaagata cgttttcaac tcggtattcg tagctgtagt agttacagtt gcacagctgc
     2341 tcttctcatc atcggcggca tatgcgcttt caaaggttgc gttccccggt gcgaaggcac
     2401 tcaataagat aatcgaagtt gcccttctgt tcacatctac cgtactttac atcatgcagt
     2461 acatggtaat ggctacactg ggcatgatcg atacatactt tgctatcatt ctgccctaca
     2521 tcgcaacacc tatgggtctg ttccttatgc gacagttcat gggacagctg cccgacagta
     2581 tgatagaagc cgcaaagctt gacggcgcaa gccacataag aatctgctgg cagatagtaa
     2641 tgccgaacgt aaaacccgct tggctgacac ttctgatatt cgctttccag ggcgcatggc
     2701 agattacagg ttattcattc atttataatg aggcattaaa acccattccg accgttatgc
     2761 agcagataag tgccgccggt atcgcccgtg cgggcgttgc cgcagcagca gttgtcatca
     2821 caatgctgcc gcctatcata gtattcgtat tctgccagag cagcgttatg gaaactatgg
     2881 ctaccagcgg tctgaaagac tgatctaata ttaaattata cagaaaggat gattttagtg
     2941 ccaaacatga agaaaatcgc tgcatcagcc gtttcggcta taatggcggt ttccatgctg
     3001 tgcagttcgg cgtttgccga tcagccgtat gaaggttata actacgactg gtggggcgat
     3061 cctgttccgt cacagaccgg atatgtcgtt gaccgagttg taacaggtct ggatttcggc
     3121 tccggcacaa gatataatac cgatgcccag ctgaaggaaa tatccgatca gacaggcagt
     3181 gaagtgacaa aagaaatcac tatcggcgag ctcaaagagc ctcaggatct gttcgttgac
     3241 cgcgaaacac agagcctgta catagttgac tcgggcaaca acagaatact tgttaccgat
     3301 gtaaactact caagaacatt ccaggttatg gattatttct ataaccccga aacaaagaaa
     3361 tatgaaacgt tgaaaaaccc tcacggcata tttgtaagac acgttgaaga cgctgcaaat
     3421 gaaaatgagt caacaatgat ctacattgcc gactatgaca acaaccgtgt actcggtgtc
     3481 tatgcagacg gtacagtctt ccagacattt gaaagacctg ccgctgagtt ctatgatgca
     3541 gatgttacat tccagcccaa caaggtcgtt gtagacgttg ccggcaacgt atatgtaaat
     3601 attctgtcta tcacagacgg tgcggtaatg ttcgcaccgg acggttcatt cagtaacttc
     3661 ttcggcgcta accgtgttga acagacagcg caggctatcg caaacaagtt ctggagaacg
     3721 atcctctcgc gtgaggctgc cgcagcattt atccaggcag ttcccatgga gttcaacaac
     3781 ttcgatatcg atgatgaagg cttcatctac actgtaacgg catcaaagag tgcaactaca
     3841 gatatcttca agaagatgaa ccccgccggc gaaaatatcc tgataaacct cggtttctcg
     3901 gattacacat acggcgatat ggcttcttac tattataaga accagacata cggctctcag
     3961 atcacggacg ttgacgttga tgagaacggt acgataagac ttctcgatta cgcaaacgga
     4021 cgtatatttg agtacacgga agaatgcgac ctgctgttca tctacggcgg caagggcaac
     4081 cagaagggtc tgttcacaaa cgtaaccgcg attgaggctt acaacagcat tgtatatgta
     4141 cttgacagcc gtaagaactc tatcacgaca ttcaagcgta cagagttcgg tgacatcgtt
     4201 cacgaggcaa tgggacttta caacctcggt aagtatgaag aggcaagcgg tccgtggcag
     4261 gaggttctga cacgcgacag caactattgg ttcgcttata tcggtcttgg taactcagag
     4321 ctttcgcagg gtaactacga acaggctatg gagtacttct accgtaacag cagagccggt
     4381 tacaacagag cgttcaagca gtacagaatg cagttcataa gagataactt caatatcttt
     4441 atgataatag ttcttgttat aatagttgcg cttgttgttc tcagcaaggt aagaaaacat
     4501 ttnnnnnnnn nngtagtaaa taatgagatt tgatttagat atgcccgctt ggaaatggcc
     4561 gttctacgtt gcaagacatc cttttgaagg atttgaggat ctgcgttgga agaaagctta
     4621 caacacaaag gtttcgatgg tgatcgtact ttgcttcttc ctgataacgg tctgccagca
     4681 agttatgaca ggcttcctgt tcaatacgaa ctacgtcaag atattcaaca tagtaccgct
     4741 tctcgttcag acgatcatcc tgttcttcac atgggtaatc ggtaactggt cactgtgtac
     4801 tctgttcgac ggcgaaggtt cgataaaagc aataacaagc gtcagtgcat acgcgctggt
     4861 gccctacctc atcactcaga tagttgtaat tatcgcaagt aatgtactgc ttaagagcga
     4921 aggtgctttc attgtgttct tccagtatct cggtatactg tggagcgttg ttctgatgat
     4981 atcgggtatc aagaccgtac atcagtactc tgtgcctaag actcttcttg ctatcgtatt
     5041 cacggttgcg gcaatggtag ttatactgtt cctgctcgtg ctactgctca gcttgttcca
     5101 gcaggtctac atcttcggtt tctcaattta taccgagctg atgtatcgat tcagtttgta
     5161 agcggaggtg cgacagaaaa tggtaaaaat cagaaaaaag atactcgcag ttctgctcac
     5221 agccgcaatg ctcgtatcaa tgagctctgt agtagcatgg tctgacgaac agcctgcaac
     5281 agacgaggga acttcacaga cagagcagac agcagcagac gataattctt ccgaggataa
     5341 ggaagataag tcagacaaga aaaacgataa ggacgacaag aagaagccca gaacagacgc
     5401 tgaaggtctt gctgagatga aagtaattgc cgagaacgac agctacaagc tttatctcaa
     5461 cgaggaaaca acaaactttg cgttacagtc aaagagcgac agctatgtat ggtgggcaac
     5521 tcctctgaat gcagataagg atgaaaacgc caagactgct cagaaaaaga atatgcagtc
     5581 gcctttctat atcgtatacg gcgatgtatc aacacataca agccagagaa tgaatgttta
     5641 cgacggttcg ataaagtccg gtgattttga gatccaggaa atcgaaaacg gtgtaaagat
     5701 catttatagg ataggtaaga tcgaagcgga aattccgatg acgatcacgc ttgaaaagaa
     5761 caatgtaaat gtttcgatca tttcatcgga aataaaggaa caggaagcta cagactcaaa
     5821 gggtcttgta cttcttgata tcggtctttt acagttcttc aatgcggcag gcatggagga
     5881 cgagggctat atggtagtac ctgacggttc aggcgctgtc atcaattaca acaaccagcg
     5941 tataaacgct caggcgtaca acaacgatgt atacggactt gacacatcga taggcttact
     6001 tacaagaccg tccataacag agcaggttta ccttcctgtt ataggtgctg ttactaacgg
     6061 cgaaaagaca aagcacggct atatggctat agctgagtca ggcgacacct gcgcttctgt
     6121 aaacgcaaca gtaagcggtc agaactcaac atcatataac aatacatggt ttgagttcaa
     6181 ggtaagagca gaagatactt actacatggg tactcgtaag cttacggttt acgaacaggg
     6241 caagataaac cagcccgacc ttacagtagg cttctatcct ctttcgggtg atgatctttc
     6301 atatgtagat attgctgaag catacagaaa ctacatgatc gaagaaaagg gcttcaccga
     6361 taagaccgat aatatcacaa acagctatta ccttgatttg tacggcggca ctgtaaaggc
     6421 acagtccatc gcaggcttcc ccgtaaacct tgaaacaccc gctaccacat atgagcaggc
     6481 acaggagata atcaaacagc tcaatgagct tggcgttgat gatttggttg taaattacaa
     6541 cgacttcaac ggagcaggta tcaagggact tatcacagca gatgtaaatt atgcaggtac
     6601 actcggcggc aaggatgcat ataaatctct tgctgagtat gtaaacggca tgaacggtaa
     6661 gcttttcgca agcgcgggca ttacttacat gaaggacagc ggaaatggct acagctacac
     6721 gctcaacgct tgtaaggcta caacaaaggc ttacgctacc acaaacaact gggatattgc
     6781 tttcggtatc ccgaatcagg taagactggt aacaaagacg accctgtcac cttattactg
     6841 gactgacctt tataagaagc tgataagctc attctccaat gagggaatca agactatcaa
     6901 cctcagtgat gcgacaacgt tgctctacag cgccttcagc cgcgagaact attccagaaa
     6961 cgatgcaatg aatatgctcg ttgacggata caagcagttc aaggacgcag gcttcacgct
     7021 tcacgcaagc ggagcaaacg cttatgcgct tccttatatt gattacctca ctgatgtacc
     7081 ggtaacatca agcaactttg acctgttcga ctatgatatc ccgttctatg agatcgttat
     7141 ccatggctat ctgccctata caacaaaggc agtcaacgca agtgcaaacg cagatgatac
     7201 tatcatgctt gcacttctca cagcaacacc tatccactat gatatgatgt atgctgaccc
     7261 caacgacttc acagacagcg attacgagac actcttctat tcaaactaca agggctggct
     7321 cgaaccctcg gctaaggtat acaagctcta tcaggatgag cttaaggact ttgctaatct
     7381 ccacatcaca aactacacaa gaatttccgg agatgagctt gagaccgagt ttgaaggcgg
     7441 taagacgatc aaggtaaata caagagaaat gactctcacc gtcaacggca aggacatcga
     7501 tcttgcacaa tatggactga agggagaaac agatgaataa ttcgaatttc aacccgaatg
     7561 aagaaattga agcaatgaag acgaccgatg ccgccgagac tcccgttaat gacgcaaccg
     7621 cagccgagca ggctgctcct gccgaaacgg tagctgatac aacagtatca gagcccgaaa
     7681 cagtgcagga aacggtcgaa gcttctgctg atgagccagc atccgcaaca gaaccggttg
     7741 cttataagtc ggctcacatg acagctgccg cggcagcgct caatgtagag ggcgaagagg
     7801 tggatatccg ccgcagacag cccagagtgc aaaaagagat caagaaatca cgcatctctt
     7861 acgaaaagaa gaagggactt tacggttacg gcttcattgc aatatggatc gtaggcgtta
     7921 tctatatgtt ccttatcccg atattccagt cggcatggta ttcgctttgc tttaccgagc
     7981 tcgtaacaac acccgagcag gcggctcaga gaggaatgtc ctcagccggt atttataccg
     8041 agtggaacaa tttcggaaac tatgaagagg cactcttcaa aaatcaggat tacctcccca
     8101 agcttaccga gtcactcggt tcgatggtac ctcaggtaat cgtagtaatg atattctcac
     8161 tgtttatcgc acttctgctt aaccagaagt tcagaggcag aacatttgca agagcggtat
     8221 tcttcttgcc ggttctcgtt gcaacaggtc ctgtacttgc ggtaatacga ggtgatattt
     8281 caaacaacgg tatttcgagc ggtgagcagt tcagcgcact gttccagacc gaccttgttg
     8341 accagctgct tgagttcctg ggtatttaca acctgaacga acagctgacg acaacgatcc
     8401 agacgatcac aagtgacatn nnnnnnnnng ttatggagtg ccggtatcca gatactgata
     8461 ttccttgccg cattacagca gatacctacc tccgcaaagg aagcggcatc tatggaaggc
     8521 gcaacaggat gggaattttt ctggaagata accttcccga tgataagccc gatgattttg
     8581 gcgaacttga tatacacggt catcgataca tttatcgatt ccgagaacgc cgtaatgaag
     8641 atcgtgcttg ctcagtcgag aaacttaaaa tacggccttt catcagcgat ggcttggatc
     8701 tacttcctca tagtagcagt tgcacttgca attatagtgg ctatcgtatc taagttcgta
     8761 ttctacgaaa atgaataagg aggtgtagtt gtgagcgaaa aagatttaga acagttaaat
     8821 gctgatgttc agcagactaa tgatgatgca gcnnnnnnnn nngtgttatc aagcctataa
     8881 ccgcttctaa gcccgaatca aagagagctt taaagaaagc tgaaaagaaa aaggctaagg
     8941 agctcaagaa tatctaccat gtaagcaact ggcagataat caagaattat cgtaactaca
     9001 atcagcagga aagaaaacat tacaagtaca tcttcagaag aagaattacc gacaaggtat
     9061 ggcctgtgtt ccgtttcctt attctgttcg gactcggttt cgtaattctg tatcctatgc
     9121 tctatatgct gtcaacggca ctcagacctc agtctgaaat gagcgaccct tcagtaatgt
     9181 ggatacctaa ggaagttatc ttctccaact tcgttgatgt atggatagca atggacttcc
     9241 cgaaggtgct cctacagacg atacttgtaa acgttatatg ctcgatgatt caggtcgtta
     9301 cctgcggtat caccggttac ggctttgcac gtttcaagtt caagggtaag ggacttatgt
     9361 tcggtatcgt aatcttacag atcatcgtcc ccgtacaggt aatacttatc ccactgtata
     9421 tgcagtgcag attctttgat gtgtttggtc ttataagact tattacaggc gatgcagtta
     9481 atctcgtaag tactcctatc gcactgtact tacaggcatt cttctgcaac ggtatcagag
     9541 caggtgtgtt catacttctg ttcagacagt tcttcagagg actgcccaag gatcttgaag
     9601 atgccgcata cctcgatggc tgtggaccct tcatgacgtt tatccgcgtt atggttccca
     9661 acgcaaagac ttcgttctta acagtattca tcttctcgat cgtatggtac tggaacgact
     9721 cttatgtaac gggtatgttc ttcacaaatc cctacacgat agcaatggaa attgaccgtc
     9781 tgggtacgac catgatgatt tatttcacag gtgaacagaa cggtatagcg agtgactatc
     9841 tggtatggat agaagcggga tgcttaatgt cacttcttcc gatacttatc atgtacatct
     9901 tcttacagaa gcagttcgtt gaaggtatcg aacgttcagg tcttgccaac taaatggctg
     9961 tgagccattg caaattcgcc ttttgaaaca ttggcgatat gtgcaggttc cgctgacggc
    10021 ggtttgaacg atagtgaaaa cataaattta agaggagtat cgaaaggtac tcctcttttt
    10081 atgtcgataa aagcaaagca gtaagttcgt attgcacaca ggcataagaa gtgctataat
    10141 agtaacaccc ggaggtagta atatggagct gtttgactta tacgatgatg aacgcaggtt
    10201 tacaggagaa actatagaac gcggaactcc gcttcccgaa agccgttacc atcttgttat
    10261 acatatatgc attttgggca gcgatggcaa aatgcttata caacagcgtc aaccgtttaa
    10321 aaagagtttc cccggtaaat gggacgtatc ggtcggagga
//
LOCUS       DAWAOG010000011         9278 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1179_length_9278_cov_1.949799, whole genome shotgun sequence.
ACCESSION   DAWAOG010000011 DAWAOG010000000
VERSION     DAWAOG010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1179_length_9278_cov_1.949799"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1435..1444
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4052..4061
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4700..4709
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5706..5715
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2246 a         2206 c         1992 g         2794 t
ORIGIN      
        1 ccgcaagggc tgatagtcac aagataatta caagtagatt atacactatc atgtgtaaaa
       61 atacaagcgt tcattaccgc tttacgggat ttttacagct gttatatact gaaaacaagt
      121 acaccgtaat caaacggtga aagcttgatc gtcttttcat cgctctcatg aggctttcgc
      181 aggaaacgag gattagtgta ttcgtccttc atatagtcgt caaggttgaa cgttctggga
      241 tatttcgact tatttaagaa tataacggcg gcttcacggt tgagcttgtc aattcttgca
      301 aatacgcact cctcaggcgt acagacgaga aacttcattt cgccctgtgc aaatgccttg
      361 cttgactttc ttatctgcgc gagctgcttt gtgaaatcta tcatatccag attttctttg
      421 ccccacggat aacagcgtct gttgaacggg tccttatatc cctcaagacc ggcttcatcg
      481 ccgtaataga tacacggtac gcccggcagg aagaattgca gtaccatagc gcacttaaga
      541 agtgacagac cgtatacata ctgctccgga gaaagatagc gctcagcctg ccattctctg
      601 tcgtgccagc ctacatcctc gcctgcaagt cttgtcagta tacgctcggt gtcgtgggtc
      661 gacaggaagt tcatcagcat atcggcactg ggcttagggt aatgctcaag tatagtcatt
      721 atgccgtcct caaagccctt tgagtcaccg tacttgcaat agtttataat aacctctttg
      781 aaaggatagt tcataacgct gtcaagctga ccgccgataa gataacgtct gcggacaccg
      841 tagctctcct tgtttgaagc gtcttcccac acctcgccgt agatgacctt atcattgccc
      901 ttggctttga ctgctttatt gagattgtct aagaactcat cggggagctc atcggcaaca
      961 tcaagtcgcc aaccccttgc gcctttgtct atccagcgtt gtaatatgcc gttttcaccg
     1021 cagatatatg cggtgtagtc agggttgttc tcgtttacgt taggcagagt agtgatgccc
     1081 caccatgatt cgtactcaat gggatagcgc gagaagcagt accagtttct gtagcgactg
     1141 tcgggatttt tatatgcacc ctcatgctcg ccgtacctgc cgaacttgtt gaaatagata
     1201 ctgtctgcac ctgtatgcga gaatacaccg tcaaggataa tatcaatgcc gtatgttttt
     1261 gcttccttgc aaaggtgctc aaagtcctca tcgctgccga gcatcggatc gatgtgctca
     1321 tagcaggcgg tcgagtagcg gtgattttcg tgcgactcaa agatcggatt taaatataag
     1381 caggtaacac ctaagctttt gagatagggg agtttgcttt cgacaccctt aaggnnnnnn
     1441 nnnnttgcgg ataatgcctt tgtcgtccgg gcggaagaaa ggcgtatcct cccagttctc
     1501 gtggattatt ctgtcagtcg gaacattttc gtgtttttca ccgctgcggc aaaatctgtc
     1561 ggggaatatc tgatacatta tcccgccttt tatccagtcg ggagccgcca tatccttgtc
     1621 aaaaacagta agctggaaca gctcgccgtt gtccagaacg ccaagccttg ctccgctgcg
     1681 ctttacatat cttctgttgc cgtcgatcat caatgagaag tagtagtgat gcagaccgat
     1741 atgcagaggg ttgaagttgc aggtaaatac gttgacatcg gtggtgctgt ctgttatctc
     1801 aagcggtaga aagcgttctt tttcgccggg acgaaacatt accagggtag ggtcggtaac
     1861 atgccattcc ttcgggaaac gcagggaaaa agtgctgggc tgatactgtc ttatcgctcc
     1921 gaacgggctt ttatagttcg ggtcaaagca gtcaaatata ggagtatcca ttaaatacat
     1981 tcctttcgga ttaaaatcgt gtgggtcggg atcgggttat atatacccga aaaagtccgt
     2041 tgctcacaac ggactttttg ctttatgtcg ggtttatcga tcaaaataat ttccagatgt
     2101 cgcgcgcata gtctgtaaca gcacggtcag ccgagaatat acctgcggaa gcgatatttg
     2161 taagggacat tttgttccac tgatacttat ccttatatgt ttcgcttgcc ttagcctgca
     2221 ttcctctgta gctgtcgaaa tccgcaaagc acatatatct gtctgcgtga cgcagtgaat
     2281 ttgctacttc ctcgaatgtt gcaccgttta tacccttgta catcttttcg aggacgctct
     2341 tgataactgc gtgtgagttg tatatctttt cggggttata tccctcagcc ttaagctgat
     2401 ttacctcggg agttgacata ccgaagatga agatgttgtc atcgcctacc gcttcgtgta
     2461 tctcaacatt cgcaccgtca taggtaccca ttgtaagagc accgttgagc ataagcttca
     2521 tattacctgt acctgaagcc tctgtaccgg cgagagatat ctgctcagag atctcacttg
     2581 cgggcataag tatctctgac agagttacct tgtagtcttc aaggaatata acagagagct
     2641 tttcgttaag cttcgggttc ctcttcagct cttcgccgat gcaccagatc atcttgatta
     2701 tctgctttgc catatagtaa ccgggagcag cctttgatgc gaatatatag gtcttgggaa
     2761 cgaagtccgc atcgggatta ttgagcagat aaagataatc tgcgataata ttcatcgcat
     2821 ttaactgctg acgcttgtat tcgtgcagac gctttacctg aacatcaaag ataccgttgc
     2881 agttgagctt tgtacctgtt gtgttgtata catactttgc aaaagcctgc ttgttagcaa
     2941 gctttacagc ggctaactta tcaagcactg ccttgttgtc cgcaaacttc ttgaagtctg
     3001 caagctttga cgcatccttc ttgaaaccgt cgcctatgca ctcggtgagg aggttcgtaa
     3061 gtccctgatt gcttgcaagt aaccaacggc ggtatgcgat accgtttgta acattcttga
     3121 atgcgtcggg agtaacagta tactgatcgt ggaatacgct gtcctttatg atctccgagt
     3181 ggagctttga aacgccgttt accgagctgc ttgccgcaca gcagagatta gccatcttaa
     3241 cacggttgtc gcggataatg gacattcttg ttaccttttc gctgtcgccg ttggtaactt
     3301 ccataaggtg agcacagtaa cggttgttta tctcaactat gatagcataa attctgggga
     3361 gtatcctctg catgagatca acgtcccatg tttcgagcgc ttctgccata accgtatggt
     3421 tggtatagtc aaatgtgttc ttgatgatgt tccatgcctt atcccagtca tatccgcagt
     3481 catcgagcag tattctcata agctcgggga tcgcaagagt agggtgcgta tcatttatat
     3541 ggatagctac cttttcgtga aggttttcaa gcgtaccgta aacattcata tgacgcatta
     3601 cgatatcgcc tacagatgcg gcactcataa agtactgctg acgcagacgc agagccttac
     3661 cctcaaggtg attatcgttg gggtaaagta cctttgtcag tgagtgtgca atgttgtttg
     3721 cgccgatagc ctttgcgtag tcgccctggt tgaacatatt catatcgaac gacatacttt
     3781 cggcactcca aaggcggagc tttgaaacac ccttgctgtc atatccggat acatacatat
     3841 cataaggaac agcgtttacc gttttgtagt tgacgtggct tatgctgtga taattttctt
     3901 cccacttctc gtggatctca ccgtcaaagt ggacctcaat agcctggtcg ggtacaggaa
     3961 cgagccatgc tctgccgccg ggtaaccatt catcgggcat ctctgtctgc cagccgtcta
     4021 tgatcctctg cttgaaaata ccgtattcat annnnnnnnn nattcataaa gaattgaata
     4081 tcctgttgcg ggaaatgcgc aggttgccag tccgtccaga tagcaggctg ccagtcttcc
     4141 aagaccgccg ttgccaagac ctgcatctgg ctcttcttca tatatatgct caatctttac
     4201 atcaagcttt ttgagagctt tcgcaacctc gtcctgcata ccgagattat agaggtttgt
     4261 ctttaatgaa cgtcccataa ggaattccat tgaaagatag tagacctgct ttcttccctt
     4321 tgaattgcag tctgtcataa acatcgagcg cttttccttg agatagttta caactatctt
     4381 tgataaagcc ttgtagatct gagtgtgcgt tgcctctgcg ggagatactc tgaaatcata
     4441 ttcaagcacc tccgtcagtt tgtgtgttat ctcattttct gtaaatggtg aagtcatatt
     4501 gttttccctt tctgtcgtcc gattttctta cagtctaatt atacagcata tgtagcttat
     4561 attcaaggct gaataaaata ttttgtaaat aataaggtga aattgcacta aatccgattg
     4621 ctgtttttat gcaatttcac aacaattatt ttctgattaa ggatttattt acgctgaatt
     4681 gacaaagatg gcgttacatn nnnnnnnnng cctttatctt aaacgccttt aaaatgtcgt
     4741 tcttgtcctt gatcttcgac aatatcataa gcttgtcgcc gacctccagc gtagtgtgac
     4801 cgcgtgggat tatcagttcg gtttcatttc ggataatcga gatgatattg agcgtcagcg
     4861 gtatttccat ttccatcagc ggaacattct gcaacgtaaa gctgtcgtca agcactatct
     4921 caaacacccc tgccttgccc gcgctgagcg gcagcagttc ttttatctta ccgctgtcta
     4981 tctcacgctc aagctgtcct gtgatgttgt cggtggagct tacaactata tccactccga
     5041 gttttttcag agcgtcaacg tttttggggt tgtttacctt tgctattgtc ttctttacct
     5101 gatacagcct ttttgcaagc tggcaggcaa caagattatc ctcatcgcgt ccagttacgg
     5161 cgataacgct gtctgcgtcg gcaacgcccg cctgctcaag tacggatacg cttgtaccgt
     5221 cgcctcttat aactgttata ttgaggtcgt ttgcaaactt ctggcaagct tccttttctt
     5281 tttcaactac cgatacatca tagccttttt ccatgaggac cttggtaagg tagtagccta
     5341 tcttacctcc gcctattatc agcgaatgca taactatagt tcctttctgt cagatagtct
     5401 ttgagaatat gagcttgtcg ccctctttca tgacgacatt gtagttattt actatctcca
     5461 ttgtgctgtc agctcttatt atagcgtaca gtgtttcgtt ttcctcatat tcgatatcca
     5521 ccggtgtcat accgataaac tctttaggta tctccattgt ggtgaaacgc accatatgag
     5581 agccgaagct gacatacttc tcttcaagca taggctttat ggcagagcag atagcctcaa
     5641 ccgtaagctt tgtcgggcat accgtttcaa gtccgaactg tgcgaagata tcgcctcttt
     5701 cagggnnnnn nnnnnatacg gctgacgacc ttctttacgc catacacctc ttttgcaagc
     5761 tgggatagca ttatgttggt gttgtcgtcg tctgtaacag cgcacacgac atcacagctt
     5821 tctattcccg cctttttcag gttgtcctga tctatcggaa ctccgcataa aagactgccc
     5881 ttgaaatcgg ggtcgagcat tttatctgct tctgccgatg ttctggctat tatagaaacg
     5941 tctatccctt cttcgccgag cgtatttgca agtcgtgagc cgaccttccc gcaaccgact
     6001 accagtacgt tcattaaaaa ctcaactcct ctccggctct caggtattgc aacctgtcgc
     6061 cgagtatatc tttcattata tcataacctt ttctgcctgt gttatggcag ggatatatac
     6121 tgcccgtgcg gagcgcggaa agctcttccg cacatttcgt tatgtatcct tgtgtgcata
     6181 tctgttttcc cgaggcattg cagtatccga agcaacccac caccgacagc accggtgcat
     6241 cccataattt ctgtacagtc cttacaatat tcgctatacc gcggtacgca ccacctgtta
     6301 tcacgacaaa tccgtcacgt cttctttttg gaaagcacac cgcaaagcat tcttccgaga
     6361 aatcatctgc aacataaaat ccttttttct ttatataata atgtctgtca ctctcggggc
     6421 tttttgcgtt cccgtctgca gctacaaggt aaaaatcctt gcacacctcg gtgaaacgct
     6481 caaatcttat cgtgttatac ttgccgctct tataaaagga ctgcggcagt ccgctgcgaa
     6541 ctctcagcaa cttttctttt tttgcgcagt cgcacgccgc cgcttcgcgt atgaaaatgc
     6601 ggagctcagg gcatttcttc gcggcgtttt ccgcaccgcc ggtgcagtcg tcggtgttta
     6661 taggcaatac taacgtatcc gttttatctg ccggcacacc gagccgctcg gcattgctca
     6721 taaacagtga cgaagcgccc gcgccgaaga gtattctgtt ttcgccgtgc tcgatataca
     6781 gacatatacc gtgttcggaa aacagcttcg gatcggctgc ttcatcatcg gacagggata
     6841 ctatcctcaa agcgcaacag tcctttcaat gtaattctat aatattctac cacatattta
     6901 ccataacttc aagcataata agttatccgg caaaaaaatg acggcagaac tttcattctg
     6961 ccgatttaat tcaaataaaa cgaaacaaac ttaagtaagg aataaaaatg aaaatcagat
     7021 attcaactgt cgccggtagc atcaggcaaa cagcgaataa gcataaagtt aaatcagctc
     7081 agtatctgga gcagtcgttc tataagcata cctatctggg gctttgagaa ctgttcgtcc
     7141 gcacctaccg aaagaccctt ctgatacatc tgctcgttta tgagagatga gaacaggaat
     7201 acgggtatct ttttgagtgt cgggtcggat tttatgagct ttgtaagatg atgaccgtcc
     7261 atctgcggca tctcgatatc ggtgattact gcggctacgc gttctgttac atcgccgttg
     7321 taatttgaaa actgtgatat gtagtcccat gcctctttac cgtttgggaa agagcgtatt
     7381 tccttaaagc ctatatcagc cagactgttc actatcatct tatttaagaa ttgtgaatct
     7441 tcggctatca ctatatgctt ttcagctgta gcggcaccgg ctttcacatt ttccgcacca
     7501 tttaagtcaa gccctgcagt cctattgata tcacttacta tcttctcaaa gtcgaggata
     7561 acaagaattt ttccgttaac tttcgctatg ccggtagcta tgccggagtc tgcgtcgcct
     7621 gaaacctgcg gaggcttatc aatggcggtc cagccgatac gctgaatgcc ttgtacagcg
     7681 gtaacatgga aacctatatc cataccgttg aattcacata ttatgaacag acctcttgcg
     7741 tctgcgggct tttccgtgcc gagcactttg tgcagatcta tgaccgtgat aagcttatca
     7801 cgcggcataa atacgccctc tatctcatcc ggtgcctggg gcatcggaac aagctcctgt
     7861 tccatcatta tttcgcgtac cttagcaatg tttatgccgt aggaattgcc gcaaatatta
     7921 aattccaaaa gttccaactc attagtaccg ctttcaagca gtatgctggt atcgtttgca
     7981 ttgtaagctg ccataaatac accttccgtt gcctctgtat tcttaattcg ttatttatta
     8041 tacaccatat ccacaaaaaa gtaaatagag aagtttcact tttagttaaa aacaaccaag
     8101 tttttgctta ctatttgtga aattgcacaa ctcacccaat aaattccttt atgagagagt
     8161 tttcgggtac cgaattttcc ggtacaggga ttatctgcga caggaactta ggcagttcgc
     8221 tgtagcggtt tacctgacgt atacattcat caagatcgtt atcacacagc cttgacagaa
     8281 cattccggta tactgaaagc tcatacggga aaaatgtatc tatatcaaag tctgcaagct
     8341 gtttatacgg cattatgtaa agactttcgc cgcgctgaac gctcgggaac tgctctatag
     8401 tcctgtggta gtcccgtcct ctgaagagct tgtcacgcga cagtctgcgg ataagacgta
     8461 tctttgaggg gtggagaagc tcaccttttt catcggctat tcttgtgcgc acactgacgt
     8521 agataaatgt ggcatgatcg tgcattctgc cggtcacatc ggggttcagt gcgtgtattc
     8581 cttcgatgat aacgacggta ttgccgtcac agcgtacttt attgccgcaa cgtcttgaat
     8641 tatcggcgaa attgaacttc ggatgctgca cctctccgcc ggaagccagt gtacgcagat
     8701 cctttttcag aagctcgcag tcgacccttg caggtgcttc aaggtcgact tttccgtttt
     8761 catcgggtgg tatcattccg ggtacaaaat agtcgtccat tgatatcact acagctttta
     8821 ttccctttgc cgtaagctcg gaggcgatgc gataagcagt agaggtcttt cccgaacctg
     8881 acggacctga aagcagtacc aacggttttt cactatgaca ctgtgctatt gtgtctgcgg
     8941 cggcttttat cttgtcggca tattgctgtt ccgatgtttc tataaattta tgtatatccg
     9001 atgcacggcg attgagttcg ccgatcgtta tcgttttgat ctgttttgtt gactgtgaca
     9061 taaaaaggtt accttccgtt atgagaattg ctagctatat cataccacaa cggaaggctc
     9121 ttttcaagat ttaattacag atcgtcggca tcctgcacag gtttttcgta aatgttgtca
     9181 catactcccg tgtagctgcc gtccgggtca aagtcggatg agcgcgtagc cgctacaccc
     9241 atagcttttc tcataactct tgccgtatcg gtataacc
//
LOCUS       DAWAOG010000012         8708 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1245_length_8708_cov_2.043106, whole genome shotgun sequence.
ACCESSION   DAWAOG010000012 DAWAOG010000000
VERSION     DAWAOG010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8708)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1245_length_8708_cov_2.043106"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1440..1449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5314..5323
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6489..6498
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6844..6853
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2702 a         1554 c         1890 g         2522 t
ORIGIN      
        1 caatatatct gaacggattt ggtgcgggtt tcagcgatga tgacataaac tctgtaaagg
       61 aaaaagcatt taatgaatgc aattgcgttg tgcttaacga gagcaactgt gatgtcaaca
      121 agaacaggtt tacaaaagaa gcttacaaaa attcagaatt taagcttctt ggtgcgtatt
      181 ttgctttaga gaatatttat gaaaacggcg gtatttatat tacaaagaat atagatattg
      241 agcgtccgtt caatagcctg agatatttca acgccttttt tgcatacaag aatataacag
      301 aattttctga agaaattttc ggcggcataa aaggaaatcc tgtgataaaa agcataatca
      361 atacatttgt tgaggaaaga tataaaaaca tttttatgcc gcttggtgag cgcattacta
      421 acataatatg cgtaaaatac ggggttaaaa tgaatggtac taccgatctg ttcagtaacg
      481 gatttgcgtt gctcggacct tcggtaacgg tggccgatac ctattacgga caggataatc
      541 ccggcaattt tcatgtttgt tcgcacaatt ataaggacgc agactcttcg gacaaacaat
      601 atataacgct cgaaaaagcg tctgttgccg ctttatcgtg cagcgcttcc ggaaacgtaa
      661 ttgtcaacag aaaagaagac tcacaggaat tgcagttcta caagaacaga gtatcgctta
      721 ttgaacagtc cggtgcttat cggttggcag tccttttagg aaggataggc aataagttaa
      781 aggtattcaa gtggctcgga aaaaaattat ttaaataatt aaacaaaaaa atcgacagcc
      841 gaaaagtgtg aatgatgagt atgtcggagt taaacactaa catggaaacg aataaagaac
      901 agctttatat ggagctttat aaaaagcagt caaagctggc atggtatgca agtgaaagag
      961 cgtccgcgac cctgcacaag gcgtgggaaa aatgctacgc attagcactg atggcagcgc
     1021 cgttaccgga gtgcgaagca aatataaatg agcttttcta tgcacgtgat aagcgcataa
     1081 aacgcatttt ccccgaaagc gtaaacattg catatgcgtg cagcgaacag tttgcgcctt
     1141 tctgcggagt atcggtattt tcgttgatag ctaattcgga taaatgtaag aattatgata
     1201 tactgatcat gcacgcaggc aatctatcgg agttaaacaa aaagcatctg tgtgctcttg
     1261 ccgctggaaa gcctaatatc agtatccgga taataaattg tgctcaggca tatatagtaa
     1321 aagaatgccg attaagaagc tatttttcag ctgaagttta cttcagatgc cttttaatga
     1381 cagaattttt tgccgaatac gacaggttcg tatatcttga ttccgacacg ataataaacn
     1441 nnnnnnnnnt tatataaccg taatatttca ggaaagtcga tagctgccgt gagcgacttg
     1501 tgtatgcaat atcttgttaa ttcaggagca aaccttgtgc ttaatggcac tttgctgagt
     1561 tataaatatt atatcagtcg gtttttgagc ctggataact catcgcagta cttcaattcc
     1621 ggagtaatgc tgatggatac aggcaagatc aggacagaag gcttatgcag caaagtattc
     1681 gcaatgcttc agagcgggga ttattattgc tttgagcaat gtgtgttaaa caaagttttt
     1741 aagaacagtg ttgaacgcat ttcgcaaata tggaatgtgc aggatattac caacgtgtat
     1801 ggaaattgta atgatcatat atccgaaaac acactatcgc atctgaaatg gtgtattccg
     1861 aggtataaga taatgcatta tataggtcct gataaaccgt ggtacaaacc ggatgccgaa
     1921 agcagtcggg tgtttaccgc atatgcatat ggaacagtat ggttcaggac aataatatat
     1981 gcttcgcttg cagacagagc ggaagagttt ttacagcatt accacggcag accaggctaa
     2041 tatggtttat cgttggaaat aggtcatata aacagaaagg aatttattac aatgaaagtc
     2101 gtacttttag caggaggttt tggcacaaga atatcggagg agagccatct gaagccaaag
     2161 ccgatgattg agataggtgg aatgccgata ctctggcata ttatgaagtc ctattcttat
     2221 tacggattta acgaatttgt gatatgctgt ggatataaac aacacgtgat aaaagagttt
     2281 ttctcgaatt attttcttca ccgttctgac atcacatacg attttacaga tcataacaaa
     2341 ataaagattc acaataacac gtctgaaccg tggaaggtaa ccgttgtaga taccggactc
     2401 aatacaatga ccggaggaag aataaagaga atacagaaat acatcggcga tgagccgttt
     2461 atgcttacat acggtgacgg cgtatgcgat atcgatgtaa acaagctttt agaatttcat
     2521 aaagaaaaca agcgttatgt aactatgaca gcaattcagc ccggtggacg cttcggtatg
     2581 cttaatatcg atgatacgga cggcgtgacg gcttttgctg aaaagaaaaa agaggatggc
     2641 ggttggataa acggcggatt tatggttgtt gaaccggaag tatttgccta tattgacggc
     2701 gattcaactg tatttgagcg tgagccgctc gagaagcttg cagaagaaaa tcagatttcc
     2761 gcctataagc attacggctt ctggcagtgc atggatacga tgagagataa agaatatctg
     2821 gagaagcttc tttccgaaaa caatgctccc tggaaaaagt gggactgaca tcaagcgtta
     2881 tgttttatag cacaggaaag gaattaaata acatggattt atcattttac aaaggcaaaa
     2941 atgtccttgt aacaggacat accggcttta aaggttcgtg gctttgtttt attctggaga
     3001 aactcggtgc taatgttacc ggatattctc tggaatcgcc tacagatcct tcacttttca
     3061 aggtgtgtgg gatagagaag aagatcacgt cggttatcgg agatatccgc gatctcgagt
     3121 tgcttaaaaa gacatttgaa aaatcacagc ctgaaatcgt gctccatctt gcggctcagc
     3181 ctatagtaag gacaagctat caaaatcctg tatatacata tgacactaac gttatgggta
     3241 ctgtaaacat tcttgaatgt gtacgccttt tcccttgcgt gaagtctttt cttaatgtaa
     3301 ctaccgataa ggtatacgaa aatcgcgaat gggaatgggg ttacagagaa aatgaaagac
     3361 ttgacggtta tgatccgtat tcaaacagta agtcatgctc ggaactcgtt acaagcagct
     3421 acaaaaagag ctttttcgct gatgatcgca ctgccatttc gacagcaaga gcaggcaatg
     3481 taataggcgg cggcgacttt tcagatgaca gaattatccc cgattgcgtg agagcacttg
     3541 aagcaaataa ggatattgcc atcagaaatc ctcattcaac aagaccgtat cagcatgttc
     3601 tcgaatcact ccatggatat cttttgatcg ctatgcgtca gtatcaggat atcagataca
     3661 gcggaaacta caatatcggt cccgatgaat gtgattgcgt tactacaggt gagcttgttc
     3721 agcaattctg tgataaatgc gacggaaagc tcagctggat aaactgcgca gagcaaaatg
     3781 caccgcatga agcgaatttt ctgaagctcg actgctccaa aatgaaatca gtatttggtt
     3841 ggaaacctgt atggcatata agcgatgcac ttgaaaaagt agcagagtgg actatggcat
     3901 atcttaacgg cgaagatgta aatgctgtta tgaataaaca gatagatgat tatcaggaga
     3961 aatttaatgt caaataatgt ttttctcgta aaaaacggag taaaacaatc agttccggaa
     4021 aattacttca agggacttga tataacgttc aatggcgacg ggaatactgt taccatagtt
     4081 gatgggtatt catttaacaa tttccacatt atggtttcgg gggagggtaa ctcaatctat
     4141 ataaatatag gtgctgatat taaaaacacc agtattttga tgagtacacc tgtaaataac
     4201 agacgagtaa acataggaaa gaatttttgc tgtaacggag ctatgctcac tttgcctgag
     4261 tcgggaaaca gaatagagat cggcaacaac tgtatgtttt cttttggcat acagctcagg
     4321 acagaagacg gtcacgctat ttacgatatt acaacaaaac aacttatcaa caaaggtgga
     4381 agctgtatta tcggaaacca tgtgtgggtt tgttgtaatg tcttatttat gaaaaataca
     4441 tttctaaatg ataatagcat tgcggcggca ggggctctca taacgaagaa gttcaccgaa
     4501 acaaatgtaa tagtcggtgg aaatccggct aaagtaataa agactaatgt caactgggac
     4561 agaaaatccg ccgagcaata tgcaaaagat tgtgaagcgc aaaatctaaa agggggtcat
     4621 atcattgaat aaagcaatag ttaccggtgc gaacggcttt gtaggaagtg cactggtaag
     4681 ggaattacta gcgaaaaata ttgaggtcgt tgctattgat ctcaatggct gttgcggaaa
     4741 cttaccggaa agcgatttgc tcacatttat tccttgcagc atagatagta tcgcggatat
     4801 agaatccgat gaacgcgtaa aaaacagtga tgtcttttat cattttgcat gggtaggttc
     4861 agcaggagag gcaagagcaa actgcagcct tcaactggaa aacgccaaat ggacgataga
     4921 ttgcctcaga tctgccaata agatcggctg tacatctttt gtgtgtgcag gtagcataat
     4981 ggaacaggaa actatcgctg ctgtttatac aaatggcaat aagccgggcg caggatacat
     5041 atacggaagc ggtaagcttg tggctcatac aatggcagcc agcgttgcaa ctcagcttgg
     5101 aataaagctt atatgggcga atattacaaa ttcatatgga cccggagagt tatctccaag
     5161 acttgtcaat acaactttgc gtaaaataat tgccggtgaa tccccctcgt ttacgtccgg
     5221 tgtgcagaat tatgatttta tttatattac cgatgtggca aaagctttcc gtcttttggg
     5281 tgaatacgga catacgcatt gcacatatgt aatnnnnnnn nnngtgcgaa tggttttgtc
     5341 ggcacctggc tggtgaaaaa actttgtttg cagaacgtgc aagttaccgc aataatcaag
     5401 gataccaatg aaaatataga tgatataaag caattaccgg ggcttaagat agtttattgt
     5461 gagttatctg aaatcgcaaa tcttgttgac cgcatcgatg agagaggttt tgattgtttt
     5521 tatcatcttg catgggtcgg ctcggcagga ccgctaagag caaattacga aacacaactt
     5581 cttaattcaa aatacagttg tgatgcggca aaagcggcga aagaactgga gtgcaaaaag
     5641 tttctttgtg caggcacaat aacagaacgc attattgata atgttgttga ggacagttcc
     5701 atcggcgtat ctcagaatat gatatacggc gcagcaaaga aaaatactcg tattttgctc
     5761 aacatattct gtcgctcaat aggaatgccg tttgtatgga tgcaattttc aaacatatac
     5821 ggtccgctca ataaatcggg aaatcttatt tcatatgctc tcggtgaatt aaaaaaagga
     5881 aatattccgg agttttccca aggaaaccaa ccatatgatt ttgtttatat ttccgatatt
     5941 gtgaatgcca tctatctgtt gggtgaaaaa aaggtcaatc atgattttta ttttctcgga
     6001 agcggcgcgc caagattgtt aaaggattat cttgtgcaaa ttccgaccgc aatgggggag
     6061 ggcgctgaaa ttgcgttagg aaagcgacct gatgatggaa ttgtttataa tatggagtgg
     6121 ttttccatcg atgaattgaa aaaagatacc ggctttaata atcagtattc ttttgaagaa
     6181 ggactcgctg aaacggtaaa atggataaag tccatataat tttaatattt gtaagatata
     6241 aaaacaacgc aaaaatcact atcattacag tatatataaa cagcgcacag caggtactcc
     6301 gctgtgcgct gtttttgtgc actgttatgc tattttcttt tcgttactgt agactgttac
     6361 ggtttcttta tgaccgttat aataagtgac agtaaagtca gacttaactc tgtatgtacc
     6421 gcttgttagc cctctcttcg cattggaaca tattgctgta ttgtcatacg ttgttaagtt
     6481 ccacttgcnn nnnnnnnntc ccagaaccac aggaaagagt gcttttcgag tgtctgatca
     6541 attctgacgg atttgatcga agtgtctgag tcgaaaaacc tgctggtaaa atcagctctg
     6601 cctgacgaga tcgtcaggac gcaggatttt gttgctgtgc tgagatcata ggtgtctgca
     6661 gcagcaaccg aggctgttac acatatgccc atcatgatgc atacggccaa aagaatggat
     6721 acaaattttt tcatacgctt attctccttg aattttaata ttagagagtt acccctctaa
     6781 tattagaacg tatgaaaagc gaaaatctta cataaaaatt tgaaaaaaat tattattcta
     6841 cttnnnnnnn nnngttctat tatagaatcg cccatttccc agataagcac tttatcatgc
     6901 ttgtaggtct caaaatagaa ggcatcttta ttgccgccga tttcgatccg ttcgggatac
     6961 gttatagcat tttccgtatt gagtcgtgct atcccatacg cattttttaa tgtttgtgaa
     7021 aaaataattt gccgttcgtt tgttttgctt ttatattgca cagcgtacat atattcgata
     7081 tcgccgataa tgtcttttgt atattgagag aagtccatta caaggcggta tttttcggca
     7141 aacgctatcg gtccatcctc agcatcttca acggtcatca tagagaaaat atcatactgt
     7201 tttattttga acataccgag attacgggtt atgactgtaa ttccgccggt aagcaccgcc
     7261 atagtgatta taataatggc ggcaatcagc agacggcgct taaagggtat aggtctgcga
     7321 ttttttgttt taaccgattc ggtattgtct gtgatcagcg ttgatatccg gcggtcgagc
     7381 tcgtgtgaga tcttatattg aggcagccgg tcgtaatctt ctatatcaga tatgagtacg
     7441 tttttaagca cctgtgatat tatgatgtct tcattcatat ttattttctc ctttcttcgc
     7501 tgccaaaatt cttcttgcgc gtgagatgcc ggcgtaaacg gcagaatcgc tcaactcgag
     7561 aatttcaccg atttcagacg ctgagtactg cattacaaaa tataaggtcg caatattttt
     7621 aagtaacggt gagaggtggt gtatattgtt cataaccgat tcatagtcca gtctgatctc
     7681 ggcttctttt tctgtatcgg cgacaatata ttctttcaga tcgctgtcat caaataaggt
     7741 ttcacggctt ccttttctga gcatatcata acagatattg cgtactatga tagcaacaaa
     7801 actctttgtt ttagtcgact caacatcttt tattttagac agatttttga taatccgtac
     7861 aaatgtttcg tgaactgcgt cttcagcgtc tgcgtaatta ttaaggatag acatacttat
     7921 actgaaaaga aatttcttat atgtcttgta aagccgggtt atggtgtcct tttcttcatc
     7981 agtaccgacc atagccagat atacggagag caaaactatt ccccctgtga caattcatat
     8041 cattatcata ttatttatgc tatttcaatt ttatcaataa ttattggaat tgtcaacact
     8101 cagataagaa aaatcgtggg gaatttttaa atatcaagct ttcgttgcgg tcacggtctg
     8161 aaaaaagctt gaaaatttat ctctctatat atataacgtt tgagaagcga aattcttaca
     8221 ctgaatttga aattttttta aaaaatttcc ccaagtgttg caaatacgaa aatctataaa
     8281 aaaacggtca tatctccgat ttaaggagaa tgaccgtttt ttatttattt gttagctata
     8341 aaagtgaagc tttacaatta cttcttgtca gcgatagctg cctgagctgc tgcaagtctt
     8401 gcgatcggta cacggaaggg tgagcatgaa acgtatgtca gaccgatctt gtggcagaac
     8461 tcaacagatg agggatcacc gccgtgttcg ccgcagatac cgatgtgcat atcgggacgt
     8521 gtagccttac cgagcttgat agccatttcc ataagcttgc caacgcctgt ctggtcgagc
     8581 ttagcgaaag gatcgttctc gaagatctta gcgtcatagt aagcgttaag gaacttacct
     8641 gcgtcatcac gagagaagcc gtatgtcatc tgtgtaaggt cgtttgtacc gaagcagaag
     8701 aactcagc
//
LOCUS       DAWAOG010000013         8508 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1273_length_8508_cov_3.072519, whole genome shotgun sequence.
ACCESSION   DAWAOG010000013 DAWAOG010000000
VERSION     DAWAOG010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 8508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8508
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1273_length_8508_cov_3.072519"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4365..4374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6525..6534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2498 a         1617 c         2143 g         2230 t
ORIGIN      
        1 gatcttgagg accttgaata tcagatgggt aacctgaagc ttctcggtca gaagggtact
       61 acagacacac aggcaagctt tatggagctt ttctgcggcg atgaagagaa gatagacaag
      121 cttgaacaga tgatagctga ggaaatgggc ttcaaggagt gcgttcccgt aagcggtcag
      181 acatattcaa gaaaggtcga cagcagaata gtaaatgtac tgggcggtat cgcacagagc
      241 tgctcgaagt tctcaaacga tctgcgtatc cttgcaaatt tcaaggagat ggaagagcct
      301 ttcgagaaga accagatagg ttcatcggct atgccctata agagaaatcc gatgagagcg
      361 gagagaataa catcgctttc aagatatgtg atgatagata ctcttaatcc tgcatttacg
      421 gcaggaacac aatggtttga gagaacactt gatgacagtg cgaacaagcg tatatctgta
      481 gcggaggcat tccttgcaac agatgctata ctgaatatca tgctcaatat aacaaagggc
      541 attgtggttt atcccaaggt catccgcgca agagttatga aggagctgcc tttcatggct
      601 accgaaaaca taatgatgga cgctgttatg aagggcggga acagacagga gcttcacgag
      661 aagataagac tgcacagcat tgccgcaggc aaggtgataa aagaggacgg cggagaaaac
      721 gaccttgtag acagaattgc cgcagatccc gactttatga cgacaaagga agaaataatg
      781 gcaaagcttg tccccgagaa ctataccggc aggagcgcga gccaggtaac ggcattcctt
      841 aacaaatgcg taaagcccgt tcttgaaaag aacaaggacg tactcggaga aaccgccgag
      901 ctttcggtat aacaaaccgt ttgtgaaatt tacaaaaatt cttgactaat ggaaatcgtt
      961 atagtataat attataatgt ataaaataac aggatgtgct gaggcacaga aacggagtga
     1021 aacatttgaa aagagtagca aagcctgtat tctttatagt gctggcagtt atactggcat
     1081 ttacgggcat agtatttttc ggtgttcata cccagtacgg cgatttcacg acaactgtaa
     1141 taagaggtgt cagtgatatt cgttggggta ttgatatcag aggcggtgtt gatgtaacgt
     1201 tcacatcacc ggcagattac gatgcgaccg atgaagaggt cgacgcggca aagtctattg
     1261 tagaaacaag acttgttgca aacaacataa cggactatga ggtctatgta gactacggta
     1321 acgataagat aatcgttcgt ttcccgtggc aggcagagga cgacagcttt gatcccgagg
     1381 cggctgtaaa ggagctcggt gctacggcgc tccttacgtt cagagaaggc tcggaaaaat
     1441 cgctttcgga cggtcagaca tatgaggatc ttccgcttat aatttccggc tcggacgttg
     1501 agaaggcttc tgccgtatat cagctcaaga gcggaaacag caaggaatat gagtatatgg
     1561 tgtcgctgaa gctgaaagac agcggcaagg aaaagtttgc ggaggctaca aagaagctct
     1621 catcgacttc tccgaagggc tatatttcca catatatgga cgataagtgc atatcgaccg
     1681 ctaccgtaaa cgaagagatc agcggcggcg aggctactat cagcggtaac tttacggctg
     1741 atgatgcaaa ggagcttgca gaccagataa acggcggtgc gcttcctttc aagttggaaa
     1801 catcaagctt cagtacgatt tctcctactc tcggtacagg cgctcgtgac gctatggcaa
     1861 ttgccggcgt tatcgcgttc ggtcttatta tgatatttat gatatcgctg tacagactgc
     1921 ccggtgtggt tgccgttatc ggtcttatcg gacaggttgc aggtacgttc tgtgcaataa
     1981 caggcttctt cccgttctca aacagcttta cgctgacaat acccggtatc gcaggtatta
     2041 tccttgcggt aggtatgggc gttgacgcga acgttattac cggtgagcgt ataaaggaag
     2101 agataaacag cggcaagacg atagacacgg cgatcgctac aggctataag agagcgttca
     2161 gcgcggtatt tgacggtaac ataacaatga tcatcgttgc catcatactt atgggtgcgt
     2221 tcggtactcc cgacagtctt gcgtctacta tactgaagcc cgtattcttc atgttcggcg
     2281 cttccacgga gggtactatc tactcgttcg gctatactct gatggtcggc gttatactga
     2341 acttcctgtt cggaatattt gcgacaagac ttatgacaat gtcgctttcc agattcaaga
     2401 tattcagaaa tcccgctctt tacggcggaa agtctgccaa gaagcgtgcc gctgaaaagg
     2461 cagcagcaac ggaaggcggt gcttcaaaat gaataataaa aaagtattag attttatcgg
     2521 caagagaaaa attctctttg ctataccgat agtaattgtt gcaattacga tcattttcaa
     2581 ctgcattttc ggagtacaga tggctatcga gttcaagggc ggtacgatgc tgacatacag
     2641 ccatatcggc gaaatcgata caaacgctgt aaagaaggtg gtcgagagcg agaactacgg
     2701 cgctgttacc gttactacgg gttcttcgat gaacagctcg cttgagaccg ttgagatatc
     2761 attctcttct tcagagggtc ttaccgcaga caaacagaca gctttgtcgg gtaagctgca
     2821 ggaagccttc aaggacaatg atcttacgct ggtaaacagc caggacgtta accctacatc
     2881 cggtactgag ttcttcttaa agtgccttgt tgcggtactg ttctcgttta ttctgcttgt
     2941 tatttatatt gcgttcagat tcaagaagat cggcggtctt tcagccggtg tgtttgctct
     3001 ggtcgcgctt gtgcatgact gcgttatggt ttatgcggtg tttgtgttct gccgcttccc
     3061 gatcgacgca aacttcatgg cggtcgttct taccgtactc ggtaactcga tcaacaatac
     3121 gatcgttgta tacgaccgta tcagggaaaa ccgtaaccta tacggcaact cgctttcact
     3181 taaagaactt gttaatatga gtatcactca gtcgataaca cgttctgtaa acacaaccgt
     3241 tacaactgct ttcgcagtac ttgcgatctg tgttgtatgc tctatatgcg gtgttacctc
     3301 gataatgaca tttgctatcc ctatgatggc gggtcttatc tgcggtacgt attcttcact
     3361 gtgcatctcg ggtcccttat gggtgctctg gcaggaaaag cacggcaaga actatacaaa
     3421 ttccggaaag agcgatgata agaaagatac aaaaaatctt gcttcaaaca cagcgataga
     3481 cgcaaagaag cttatggtgt aatcaccttc ggcttgtaac tcaatcgata ttaaaagccg
     3541 cacagtacat ttgtcctttg cggcttttga atttaaacat gatattctgt taaaaatacc
     3601 aaatgggaga gaaatatata ttcccggagg acgttttgaa aagagatatt acaaaaaaaa
     3661 ttacaacaca ttttaatact gtcatacaca gtccgaagct ttggcttacg cttataatac
     3721 ccccgatagc tgtaattata atagggttgg cggtgtttgc gggaaacggg ctgtatccgt
     3781 tcggcgacaa aacacttgcg tggtgtgata tgcatcagca ggtgttgccg ttgttgctgg
     3841 attttaaaga tatactcgag ggaaagggtg actttctgtt caactggcag aatgccggcg
     3901 gaatgaattt tctcggtgta ttcctgttct ttatatcaag cccgttttca cttcttgtag
     3961 tatttgtgga taaagcggat atgatgctgt ttatgaacat tatgacgctt atgaaaatgg
     4021 ctgtctgcgc aataacggca aacgcgtatt tccgcacctg ccataagaag cttgatgttg
     4081 tttattcggc actgttcagc gttatgtatg cgttcagcgg atattctatg cttttttatc
     4141 agaacacggt gtggcttgat gtaatgtact ttttcccgtt gctgctgata gcgttcaact
     4201 cgcttatcaa gcgcaaacgc ataggcggat ttatattctg ccttgtcggg atgctggtgc
     4261 tgaactatta ccttagctat atggtagtgc tgttcacgat actgtttttc ggcgtttatc
     4321 tgtttttcaa cagaaaaaag ggcagtacaa agggaatagc tccgnnnnnn nnnnttgttc
     4381 agtatctttc atcggcaaga ggcgcgaata tacttgaggg gctgtttgag tcgactttat
     4441 ttgcgggaat atatacaaat ctgccgctga tattctgtac cgggttttct gttgcggcgt
     4501 ttattgtgta ttttctgcga aggaaaagca agagtagtag gttttatgcc gttatggcgt
     4561 ttttgacgct gctcccggtc atatttgaac cgataaacaa gatgtggcat acaggcgatt
     4621 atatggcgtt cccgatgaga tacggatata tgactacgct tatgatgctg acctttgcgg
     4681 ctgtaaagct gtcgcacaca aggtcggagg attacgccga aaagagcaaa aaagggtatt
     4741 ttgccggaat acttgcggcg agtgttatat ttttcggatt tacggtatgg tattactacc
     4801 gatttaagaa tgggctggat tcatatgttt cgactttgtg gggaagcgct gagtctttct
     4861 gtctgctgat catcattttc tgcggattta cggcgctgta tgttatcacg atgtcgttcg
     4921 gagtgttcag aaagcttacg ttcagaacgg taggaacggt gcttgcggtg atagttgcgg
     4981 ctgagtgcgt gttcaatgca aacgtttata tcggtgcggc ggcatacacc ccggttaaat
     5041 acaataatgc gatggcgctg gagggcaaaa tcccacagga cgatgatttt tacagagtta
     5101 aactgtcgca atggcctaag aaatattttg acgctaatct tataggcgga ctcggatatc
     5161 cgactgttgc acactacacg tcgcttactt ccgagcatta tatgtatgct atgaaacggc
     5221 tcggctattc atcgtactgg atggaagtca caggtaacgg cggcactgca cttaccgatg
     5281 cggtgatgaa tatcaaatat tatatagaga agttcagtgc aagagagcct gttatatatc
     5341 gcgacaggaa ctattctata tatgagagtg aattttacct gccgctcggt atagtgaccg
     5401 acagcgatct gtcaaagtgt gaggaacttc ctgccgctga acgcatgatt atacaggagt
     5461 attttgcggg aaatctgctg aatacgggta atgatctgtt gtttacgaaa tacgagcata
     5521 ccggagaaaa gaatgtcagg tatacttacc gcgacggaat gtattatttt acgcctaaaa
     5581 gcgatgtcag aaccgcaaca ctcagctaca agctgaaaat agacgataaa cagtcgctgt
     5641 attttgattg tttcgatctc gttacacgca acattaagga gcatatcaac ggctcatttg
     5701 ctatatatgt caacgggatc aagaccgatc tgtcgtttcc cggacaggat tccaacggac
     5761 tgctgtatct cggaacattt gaaaatgaga gtgtaagcat agacgttgat gtaaacacaa
     5821 acacatattg ctcgagtttc ggtgtgatcg gattgtcgca tgataagctg gaaaaggcga
     5881 taagcaggac aaagacagcc gatcttactg ttgacggcgg aagcattagc ggtactgtag
     5941 aggatgccgg ggacggacag tggctgtttc tttcggttcc ctatgacagc ggtttttcgg
     6001 caaagataaa cggtcataag gctgagatct accgctgtat gacgggattt atggcgttaa
     6061 agctcgacga gggacataac gacatagcgg ttagctttct gccaaagggg cttaaggaag
     6121 gcggcgtatt gactgttggc ggtcttgtcg cattggcatt gtatataatt ttccgaagaa
     6181 agctagaggc tgtctatgca aaggttgacg gcatatgcag aataggtgtg tatgcactgc
     6241 tgtgcggagt gttgtcggta gtatatatac tgccgctcat tatctcaata atcggcaata
     6301 tatatacgac agtaaatcaa tgatcagaaa ggacgtaacg aaaatggata taggagtatc
     6361 aacagcgtgt ctgtatccgt tggaaacaga aaaggcactt tatgagcttg caaagcgtga
     6421 ggtcaaaaat gtcgagatat tcatcaactc ggtcgatgag cttgagggac aagtgcttgt
     6481 tgagctgaga aggatcatcg gggaatacgg aataagtgta aagtnnnnnn nnnncagata
     6541 tatggaacgt tatctccggc tataccgaat agcaaagcaa ttcggtgtta cggtcgcaca
     6601 ggaaaatata tgctactgca aaagcagcag tgtgaagttc attgaggata tgatcgcaca
     6661 actcggtgat gaggtaaggt ttgtggtaga tctgaagcag gcgagaagaa gcaacgttga
     6721 tcctttcagg ctgctggata taatcggaga taaagtagtt catatgcacg taagtgacgg
     6781 tgacgctggc tgtgattgtt tgccgatcgg caagggcgaa tttgatttca agcatctttt
     6841 caaggagatg gataagatct cttatgataa ttcgatgata gtagagcttt atcgtgaaaa
     6901 ttataagagc tatgacgaac ttgcaatgtg tgcaaaggat atgcaaaaac tgtataacaa
     6961 atacaaaacc ggagtataat agcaaaaaag cttgaaatag ggctgaaaat gtgttaaaat
     7021 acatagaaac caagcaaaag atcgaaagcg aggaaattgt atgaaatgcc cagagtgcgg
     7081 atatgaagac agcaaggtga tagacagcag acccgcggac aataaaatcc gcagaagacg
     7141 agaatgttta tcgtgtaaat gtcgctttac tacatatgaa atggttgaaa caatacctct
     7201 tatggtcgta aaaaaagata ataccataga accgttcgat cgggataagt tgataaacag
     7261 acttgcaagg gcaactgtca aacgtccggt tcagatagaa gatctggaaa agatggttga
     7321 agatattgtg caggaactga aaaatcagtt caggcgcgag gtgacctccg atgaaatagg
     7381 agagcttgtg cttcgcaggc ttaaggatat cgataaggtg gcatatataa ggtttgcttc
     7441 ggtgtaccgt gacttcaacg acattgatag ctttgtcagg ataatttctg agttgaacga
     7501 ggaaaaatag gatttttatc agtgccgtcc ttaaagggcg gcattgtttt ttacataacg
     7561 gaaagagcac acggtttttc aacatataga aacgcggctg ttgataacta aatattgata
     7621 gcagaaaact gtatattgtg gttgttcaca ttttgcacaa tcggggaata taatggttaa
     7681 aaaccatttg tgcaaaatta aattaaaaat atgttgacaa agctcgaaaa gcgtgatata
     7741 atagacaagc actcaaacga gagggcacga aaacagaggg tacttgagaa agcctgaaac
     7801 cgcttgaata gcgagaaaga gggcagtcga gggtacgaag aagagctgaa aaaaccagaa
     7861 ggaagaaact gcggaagcac ggtgaccgac tggtaaaaag ttcaaaactc tgaaaaaaga
     7921 aatttaaaaa atttcaaaaa agtgcttgac aaactcgaat gaatgtgata taataaataa
     7981 gctgtcgcga gagcggcgca aagttccttg aaaattgaac agtacagaat taaaaacatt
     8041 accttgagat tcttaagaga ataacaagaa attctggatg aacacaaaat aacgtgctaa
     8101 tagatagcaa gttagcgttc agcagagcaa tcaaactttg atatttaaag cgaaagcttg
     8161 aatataccat ttaacaaaga gtttgatcct ggctcaggac gaacgctggc ggcgcgccta
     8221 acacatgcaa gtcgaacggt gaagaggagc ttgctcctcg gatcagtggc ggacgggtga
     8281 gtaacacgtg agcaacctag ctctaagagg gggacaacag ttggaaacga ctgctaatac
     8341 cgcatgatgt atcgggatgg catcttcctg ataccaaaga ttttatcgct tagagatggg
     8401 ctcgcgtctg attagatagt tggcggggta acggcccacc aagtcgacga tcagtagccg
     8461 gactgagagg ttgaacggcc acattgggac tgagacacgg cccagact
//
LOCUS       DAWAOG010000014         8435 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1280_length_8435_cov_2.527566, whole genome shotgun sequence.
ACCESSION   DAWAOG010000014 DAWAOG010000000
VERSION     DAWAOG010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1280_length_8435_cov_2.527566"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1029..1038
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7118..7127
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7699..7708
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2449 a         1985 c         1735 g         2236 t
ORIGIN      
        1 acctacatct ttatggatcc cctgctccgc cttatcggcg caagcgacgc tactatcggc
       61 tatgccacag actacctctc ggtatacctt acaggcacgc tgttcgtaat gttcgcaacc
      121 ggacttaatt cgttcataaa cgcacagggc agacccggaa tttcaatgat agccgtaata
      181 atcggcgcag tgctgaacat cggacttgac ccgcttttta tctatgtgtt cggtatgggt
      241 gtaacgggcg ccgctctcgc aacggtaata tcgcaggctg taagcgcctt tgtaatagtc
      301 ggctttcttg tatccgacaa agcgactttg aaaataaagc cgaaatatct taagccggat
      361 ataaagataa tcggctcgct gttcgctctt ggcatcgccc cgtttataat ggcaagtacg
      421 gaaagtcttg tcggcttcgt cctcaacggc tcgctgagca catacggcga tatatacgtc
      481 agcaccctta cggtaatgca gagtgcaatg cagtttgccg ccgtgcctct gagcggcttt
      541 gcgcagggtt ttattccgat agtcagctac aattacggca agggcaacac cgaccgcgtc
      601 cgcctctgct tcaaatattc cgtaataatt atgttctcgt tcttcgccct gacaaacctg
      661 tttatgatca cctttcccga atttgtcgca ggaatgttca cagacgatac cgcacttata
      721 aaaacagtcg gcagaatgat gccgctgttc cttacaggta tgacgatatt cggacttcag
      781 cgtacctgcc agagtatgtt tgttgctctc ggtcaggcta aaatttcact gttcatagcc
      841 cttctgcgca aagttatact gcttatccct ctcgctcttt tcctcccgac tttcttcggt
      901 gtggagggcg tatacattgc cgagtcggta gccgatgcga cctcggcgat ctgctgtaca
      961 accattttcg ccgttacatt cccgaagata cttaaaaagc gtgagcagac gtaaattaaa
     1021 taatcaaann nnnnnnnntt ttgctttaca tttgtttaag tacttcaact tctttttcaa
     1081 actcacagtc gacccacgaa taaaggttct tatcctcata ttcacggtaa accttttcaa
     1141 ctatcatacc gagtcgttgc catatcttat atatctgtat gcggataagc tcgtctgtac
     1201 tgaactcagc cttgccgtac cctctcagaa aatccgcatt gatttccccg aagtcgtgaa
     1261 aatcgtggct cataaacgga tctccgtaaa gtatcgccgc aaagtcgatt attcccgaaa
     1321 actcaccgtc tttcaccata acgtttccgt tccatgtgtc ggtatgtata agacacggct
     1381 tatccgccgt atcaagctgt gcgcggaatt taccgataag cttaatcagc tcatcatgcg
     1441 atatgctttt cagcgctccg cctttttctt ctaaatcggc aagtagcttt tcaaaaagca
     1501 atatcacaaa atcggaattt ttattgcaga agctatcggt aatattcggt atgccgaaac
     1561 accgggcagg cataaccgtt atctgccttg tgagcttacc gacctcatat tttatgttgc
     1621 cgtactgctc tgcggtcaat ccctctgtat cattcagcgg tttgccgtct ataaaactca
     1681 tgaagaaata cggtacatcg cagatttccc cgctttcatc ataaaaaagc cgttcgggca
     1741 tagctatgtc tgtttttccg cctatcaatt tcagcatctg agcctcggtc gcaatatatg
     1801 ccgcttcgtg ccacattact ttaacgttgc tctttgtccc gaccttaagt acgaccttat
     1861 tcttgccgct ttctataaga taaacgctac tgcaaaagcc gcccgacaaa gccgtgattt
     1921 tcaactcccc acagtcggaa aatgctcggc gatgcatctt ttcaattgta tcattactca
     1981 gcttatattt agttgctgcg ttcattttat ccaccagtca ggtgtcaact ccccgagagt
     2041 tgacacctta tcattttcat agtcgagcat tatctcaatg tttttatatt cgcccgccat
     2101 aagctgtacc ataagctccc tgcacgctcc acacggcgca cccgttttac cgtccggcat
     2161 tatcgcaagc acccggttga ttttgtcgtc accgtttgtt atcatattga atatggcgtt
     2221 tctctccgca catattccga gagtggaaca ggtgtctatg caaactccgg tatagatttt
     2281 gcccgttgac gacagcacag cggcggctac gccgcctgcg gaaatatatc cgtttatatc
     2341 cctatcattc tgcaccgatt tagcctttgc gtacatttca ttccatatct tgtccatttt
     2401 tatctccgtg ttgattaaag caaatctgcg attttttcaa gtatagcatt aacatctttt
     2461 ttagtcaagc aactgtaaat tgattttcac aaacttttct cattcgttta aggttcatct
     2521 aagaaaaaat ttgtataaca cagataacaa cttaaaggag gttaagctaa atctatttca
     2581 aggcaactcc tccattttgc cgtatattac gaccgcccgc acatttttcg tgcaggcgat
     2641 gctttgcgtc tatttcagaa attccgccag tatctgaccg agtaccctgt actgctcatc
     2701 gctgagcctt tttcttactg tattgttgta ttcgtgaaaa gcgtctataa gcaagcgaac
     2761 acagtcagcc tgtccgtccg taagcccgat aagcgaaacc gttccggatt gctgtaagcc
     2821 gcacaattca tcgattgata cacataattc ttttgacaat gtgcagagcg aatctaaaga
     2881 cggactggca gtattgtttt catatttgct aatcatagaa ttactcacac caagaatttt
     2941 tgcaattctc tcctgtgtga tatttttttc attgcgatat ttcttcaacc ttgttccgaa
     3001 atcatacggt tcattcataa tcaacattcc tttaattcac tattttgatt caatcacttg
     3061 aatttaattc aagtttatgc taaaatagtt gcgttaaacg aaataatttt tgaaaaaagt
     3121 tgaattaaat tcagcttttt atctatggag caaaaataac gtgcaacaac aaataaaatc
     3181 cagacaacgt gtgaccgaac acggcgaggt tttcaccgcc gagcgtgaag taaaggcaat
     3241 gtgcgacctt gtaaaacagg aaaccgagcg tatcgacagc cgttttcttg aacccgcctg
     3301 cggaacgggc aatttcctta ccgaaattct cgcaagaaag ctgtcggtcg tgaagtctga
     3361 atacggtcgc agtcctcttg aatatgaaaa atactcgctc cttgcggtaa gcagtcttta
     3421 cggtgtcgat ataatggctg ataatgtcga aacctgccgt gaacggctgt ataagcagtg
     3481 ggacaagcaa tacagagccg tctgcaaaaa agaatgcaac gatgatacaa gaaaatccgt
     3541 ccgctttatc ctgtcaagaa atatcgtctg cggaaatgcc cttactctta agtgcgtaga
     3601 cgaaaactgc aacgataccg aacaggatat agtcttttcc gagtgggcat tcgttttcgg
     3661 cagtaatata cagcgcagcg attacagctt tgacgaactg gtaaacggca ataaggaaaa
     3721 agcttctcag ctcaccctca gcgacagcac agaaggcgag ggtacttttt tacggaaata
     3781 tatatgcaac tacaggaggt tatgggaaaa tggctgataa tatttcaacc ggaattttaa
     3841 gcggacttta cgaaaaggta tacaaccccg atgttctgtc ctgccttgcc aatctctcaa
     3901 gcgatgaggt tttcacacct ccggagatag taaacaaaat gctcgatatg ctcccgcagg
     3961 agtttttctc atctcccgat acaaagtttc tcgaccccgc cgtgaaaacg ggagttttcc
     4021 tgcgtgagat agcaaagcgt cttataaaag gccttgaagc cgttattccc gacagacagc
     4081 agcgaataga ccatattttt aaaaatcagc tttacggcat tgcgataacc gagcttacaa
     4141 gcctgctctc acgcagaagc ctttattgca gtaagtacgc aaacagtgct ttttctgtaa
     4201 cacactttga aaattcggac ggaaatatcc gtttcaagcg tatcccccac tcgttcgaca
     4261 agtccggaag atgcatcttc tgcggtgcgt ccgaaacgca gtacgaccgc ggcgaagaac
     4321 gtgaatatta tgcgtatgaa tggatacata cattacaccc cgaggagatt tttggcatga
     4381 agttcgatgt tattatttca aatccaccgt atcagttgag agatggtggt ggaacgggtt
     4441 ctagtgctat gcctatgtat catgagtttg tctatcaggc ttttaagatg aaacctcgtt
     4501 atttaacaat gatagttcct tctcgttggt ttgctggcgg taaaggactt gatgatttta
     4561 gaaatatgat gctcaattct gaaaaaatat ccgcgatagt tgattatgct aacagcgcgg
     4621 attgttttcc aggagtaact attgcgggcg gaatttgtta cttcctatgg ggctttgatt
     4681 ataatggcga atgtaaagta gtgaatatga gtgcaggcga acaaatatca agcactattc
     4741 gcaaattaaa tgaataccct atatttgtaa gagataatac agctgtacag attataagaa
     4801 aaataaaaag cgtttctaaa cagtatctcg ataatattgt tcattcaaga aattgcttca
     4861 atttgttcag caaggaaatc ggacataatg aatatataaa agaagattgt gtactgtatt
     4921 cgctgaatgg caaatcgtat atcaccaaga atgaaatatc agacagagat aatttattga
     4981 acaaatataa agtgataatg acaaaagcaa tgtcaggtgg caataaacca tccactgacg
     5041 gaacgtattt agtcatttca tcaacaatgc gtgtgcttaa acctaacgaa gtatgtacag
     5101 aaacctattt gtgtataggt tcttttgaaa acgaaataga agcaaataat ctttgtgact
     5161 atatgaaaac aaaatttttc agattcttac tgcttcaagc actgacatca atcaatattt
     5221 ctaaagataa atttttattc gtccccctcc aagacttctc aaaaccgtgg acagattccg
     5281 aactctacga aaaatacaat ctcacagatg aagaaataaa ctttatcgag tctatgataa
     5341 gaccgatgga tttaggcggt gacaacaatg gctgacgtat ttgaaaaata tgtgacccct
     5401 cgccccgagg taaacccgac tatctatgcc tacaggctga acggcgttac cactcacgac
     5461 ggctacataa aagtaggcta caccgaccgt gatgtaaagc agcgtataaa agaacagctc
     5521 catacaagtg cgataagcta caccgtgctt tgcgaggaaa cggcaatgcg tcctgacggc
     5581 tcgtgcttca atgaccatga cgtacacgcc gttcttaaac gtaacggctt tcaccgtctt
     5641 aacgaaaaca ccgacaagaa cgaatggtac aactgtaccg taaatgatgt ccgctccgcc
     5701 gtgcgtgagc ttcgcacagg cgaaaggacg gacgaatacc gcaccgccga ctttaaaatg
     5761 cgtccggaac agagtaaagc ggtagaccgc acaatcgaat actttgaaaa cattaaaaag
     5821 accgagcctg agcgcatacc aaaattcctg tggaatgcaa aaatgcgttt cggcaagacc
     5881 ttcgccgctt atgagctttg caagaagatg aatttcaagc atattcttat tctcaccttc
     5941 aagcctgccg tagagtcggc gtggagcgag gacttaagaa ctcacgttga ttttgagggc
     6001 tggcagtacg tttcaaacaa ggacgcacat aataacaagc agaatatcga cgaggcatat
     6061 tacaatgccg acagcacaag accgatagta gttttcggct cgtttcagga tttgctcggc
     6121 actaacgaaa acggcggtat aaaatacaag aacgagttta tccatactac caactgggat
     6181 atagtcatat ttgacgaata ccatttcgga gcgtggagag aaaacgcaaa gaagctgttt
     6241 gacgatccag acgatgaaga tatagacttt gacgcagaaa aatatcagaa ggaagaagca
     6301 ggaaatgccg taaacgaaac cttcctgccg ataacctcgt cacactacct ttatctgtcc
     6361 ggtaccccgt tcagagcaat gcacagcggt gagttcatag aagaacagat tttcaactgg
     6421 acttattccg atgaacagag cgcaaaagaa aactggaagg gcgaaaacaa cccatatctg
     6481 tcgcttcccc gtatggtaat gatgacctat aaaattcccg aaagtatacg ctctgtagcc
     6541 tctgagggag aatttgacgg cttcgatctg aatctgttct tttccgcaaa gggaaaaggc
     6601 gaggacgctc gcttcgtaaa cgagaacgaa gtgcagaaat ggctcgatct tatccgtggc
     6661 gcatatctcc ctgcaagcgt agacgacctt aaaattgcgg taagtcttgg tgttgacaag
     6721 cgtccgccta tgcccttctc agacacaaga cttcttaacg tactgtcgca caccctatgg
     6781 tttttgccga gcgtagcgtc ctgctttgcg atgtacaatc ttcttatgca aagccagaac
     6841 aactattaca atcagggcta taagataaac gtctgcgccg gtataaaagc gggcgtaggt
     6901 cttgatgcgg taaagcccgt tattgattct atgggcgatc ctctcgttac aaggacaata
     6961 accctcacct gcggaaagct gacaacaggc gttaccgtaa agccgtggac aggcatattt
     7021 atgctccgca atcttaaaag cccggaaacc tatttccagt cggctttccg tgtgcagtcg
     7081 ccctggacgg taaaaaatga gaagggcgaa gtagagannn nnnnnnntgt tttcgacttt
     7141 gcacttgacc gtgcgctccg ccagatatcc gactatgcct gcaatatgaa ttccgagaac
     7201 acaaaccccg aaaagaacgt cagcgagttt ataaacttcc tccccgtcct agcctatgac
     7261 ggctcgacta tggagcaggt aaacgcacag gctattcttg atattaccct tgccggaacc
     7321 tccgcaaccc tcctcgcaaa gcgctggcag tccgccctgc ttgttaacgt tgataacgat
     7381 acgttaaaac gtcttatggc aagcacagag gcaatgaccg cacttgaaaa tatcgaagct
     7441 ttccgcaatc tcaacaagga gcttgaggta atcatcaaca agtccgaggc ggtaaagaag
     7501 ctgaagaaag acggcgtgga aaacctcacc ccgaaagaaa agaaagagct taccgaagcc
     7561 gagaaggagt tcaagtcaaa gcgtaaggag gtacaggaaa agctgatgaa attcgcttca
     7621 agaattcccg tatttatgta ccttaccgac taccgtgaat cctgcctcaa ggacgttata
     7681 acccagcttg aacccggtnn nnnnnnnnaa aggatttcga ccttttgtgt tcgctcggcg
     7741 ttttcaactc cagccttatg aatcattcca ttttccagtt caaacgctac gaggacgcaa
     7801 gccttaccta taccggaatt gatatgcacc gtaacgataa ggagataggc ggttgggata
     7861 ccgccataag aaaagatgaa tataaaaagc tgttctataa tcagcaggaa tcaatgaacg
     7921 ctcctcagcc ctccgtaccc gacagggata tagcaatgct gaagaaagaa atcgcaaaaa
     7981 cgcctgcagg aaagcccggc gtccaaacct caggcacatc atcggcatat ccgtccgtac
     8041 ctccactcgt caccgaaaag ttcggcataa aatcaccaat tcccaaagcc gctttaccca
     8101 ttcccgacag aaaacgcagc aacacccccg attttgacga atcgtcaata acagtaggta
     8161 tgactgtaat acataaaaaa ttcggcgaag gaaaagtgca gtcaatcgct gataaacata
     8221 tatatgtaaa atttgctgac tgtcagaaaa atttcctgca cctgccgaaa taaccaaaat
     8281 caacagcccg actgaatcca gccgggctgt tgctgtatac aacaaaaaat cccgtgcaaa
     8341 atacacggga ttaaacttgg tacaccttca gggactcgaa cccaggaccc actgattaag
     8401 agtcagttgc tctaccaact gagctaatgg tgcat
//
LOCUS       DAWAOG010000015         8330 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1294_length_8330_cov_1.988157, whole genome shotgun sequence.
ACCESSION   DAWAOG010000015 DAWAOG010000000
VERSION     DAWAOG010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1294_length_8330_cov_1.988157"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    207..216
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    759..768
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1264..1273
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7365..7374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7891..7900
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2569 a         1659 c         2090 g         1962 t
ORIGIN      
        1 cacggtacag agtatgtcag cggcggaaac gaaataagtg acgcgtataa taacggcaca
       61 cttttatcaa ttgacggcat atcggcggac agtatgaagg agcatttcaa aaaggtgttc
      121 ccgtgcgagc cgcaggtcag cttctatgcg gactatatgt taggcgtggc tctggagtac
      181 ggcagctggc ttacgcttct cggtgcnnnn nnnnnngtaa ggtaaaagat aacgatatta
      241 aaaacgtagc tgttgatctg cgcagtaacg gcggcggaac aacagaagtc ataaacgaat
      301 ttctgcgcta tatagatatc agcgattata tgcttttcgg cggtgttgat atcagaaaag
      361 gaggaaacct tgtttcctac cgtgatgaga taacgccgaa caagcacgtt gaaaatgctt
      421 ttgacggcaa ggtgtatgtg ctcacatcgc aatacacgtt cagctcggca atggactttg
      481 caacggtgat acaggacaac ggcataggta aggtgatagg tgaaataccg ggaaatatgc
      541 cgaccgcata cggcgacaag ctgagttttc agctccctga gtcaaagctt gttctcagtg
      601 tatcgtacaa gaaattctac cgcgtggata taacaaagag catagaaccg cttattcccg
      661 attatacggt cgatgcagac aaggctgagg aaaagctggt cgaatatata aaaaagtagg
      721 agaaaatttt tcaatgggat tttttgaaaa gctgaaaann nnnnnnnnca actccatgct
      781 ccacgccttc accaaaatcg acgaggagct gtttgaggag cttgaagaaa cgcttatttt
      841 atcggatata ggtgctgtga caagcgcaaa tatctgtgag gctttaagaa aagaggtaaa
      901 ggctacaggc acaacagacc cgaatgaagt taagggactt cttaagaaga taatttccgg
      961 gatacttgat ggcgataatg agcttattct tgatacaaag ccgacagtaa tacttgttat
     1021 cggtgtaaac ggcgcaggca agacgaccac tataggaaag ctcgcatata accttaaaaa
     1081 cagcggcaaa aaggttgtcg ttgcggcggc ggatacgttc cgtgcggcgg ctatcgaaca
     1141 gcttgaggtg tggacacagc gtgccggtgt tgatattata aaacactccg agggaagcga
     1201 ccctgcggct gttgtgtttg acgctgtaaa ggcaggcatt gcaagagatg cggacgttat
     1261 tatnnnnnnn nnntattatc tgcgatacgg cgggcagact tcacaacaag aagaacctta
     1321 tggacgaact taagaagata gcgagaatag taaacaacaa tgcgcccgac agccgtgtcg
     1381 aaactctgct cgtgcttgac gcaacaacgg gacagaacgc ggtaaaccag gcaagactgt
     1441 tcaaggaaac tgcggatatt acgggtatcg ttcttaccaa gcttgacgga acggcaaaag
     1501 gtggtataat aatacctata aaagaagagc ttggtatacc tgtaaagctt gtcggtgtcg
     1561 gtgagaagat agatgatctt cagccgttta tacctaaaga ttatgtggaa gcactgtttg
     1621 aataacagca ccgtatacga aaggacaata tatgaaactg atcatcgcct ctaacaatgc
     1681 aggcaagata aaagaattta agaaaatgct gacgcctctc ggttatgagc ctgtttccat
     1741 gaaagaagca gggatagata cagaaatacc cgaggacggc acaacattca gcgagaacgc
     1801 gcatataaag gcaaaaacta tttatgatat tacccatact cccgtgcttg cggacgacag
     1861 cgggcttgct atagaatttt taggcggtgc accgggaata tattccgcac gatacgcagg
     1921 cgagaatgcc acaaacgagg acaggataaa caaagtcctt gatgagctga aaggtgttga
     1981 caagccgctc agaaacgcaa aattcgtgtg cgctctgtac tttatccgtg acgatgatta
     2041 tgaaatatgc gttacaggcg agtgcgaggg atttatcgga gaagagccta taggtaaaaa
     2101 cggcttcggt tatgacccga tattcatgct tgacgatgat acaagcatgg cttgcctttc
     2161 ggacgaagaa aagaacaaga taagccacag ggcaagagcg cttgaaaagc ttgcttacga
     2221 gctcaataaa aagtaatgga tatcaaggat atactgctat atctgctgtg ggtatacctc
     2281 ggcattgtaa atgtcgcagg ctttatactc cccgcggtag ataagagaag agcaaaaaag
     2341 gacagatggc gcataaggga aagcacgctt tttcttatat ctgcgctcgg cggctcggtc
     2401 gcaatgtata tttcaatgcg gctgttccac cacaagacaa agcataaacg atttatgata
     2461 gggataccgg taattattgt tttacagctc ggcgctgtat ttgctgtatg gtatttcttt
     2521 ataagataag aaacggagaa aataaatgga gataacaagc agacagagag caaagctgaa
     2581 aggcattgca agtaactatg aaactatctt tcagatagga aagaacgcaa tttcggacga
     2641 gcttataaag cagctttcgg acgcactgga ggcaagagag ctcatcaaga taagagttct
     2701 tgaaaactgc ggctacagcg caaaggagct tgcgggtgag attggtgaaa agacaaacag
     2761 catagttgta caggtgatcg gcacaaagat agtcctttac agacagaaca aggacgtaaa
     2821 gaaaaggaag ataagcttag atgattaaaa taggcgtatt cggcggggca ttcaacccca
     2881 ttcacaacgg acatataaat atggtgaaag aagctttcgc cgaccttaag cttcagaaga
     2941 tgctgataat ccccacttgc gtttcgccgc acaaaagcaa caagggactt atcgcttttg
     3001 aggacagggc taagatgtgt gagcttgcct ttgcagacga aatagcaagc ggcaagtttg
     3061 agataagtga tattgaaaag cgtatgggcg gtacaagcta cacgataaat actatccgtg
     3121 agcttaaaag gcagtacccc gatgacgcgg tattctatct gataatcggc ggcgatatgc
     3181 tgttctattt cgataaatgg tatcgctatg aggcacttct gggcgagtgc aaggtggttg
     3241 ccgccgcaag agaaaacagt gagtacagcg atatgtgcga atatgccgca gaaatgggca
     3301 gaataaaggt acttaacctg cacgtcactg aggtaagctc gacagagata agagaaaagc
     3361 tgaaaaacgc agagagcatt acaggacttg tccccgcaga tgttgaaagc tatataaagg
     3421 aacgtggtct gtatgtgtga gaaacagggc aaggacgaac agcgttatgc cgagaaatat
     3481 gcgaattatg taaagctcct taaagacaca ctttccaaaa agcgttttac acacagcgtg
     3541 aatgttgccg cgatgtgtct ggcgcttgct aaaaagcatg gcggtgacga ggaaaaggca
     3601 tatcttgcgg gacttcttca tgatataaaa aaggaagaaa cgccgaatgc catgaaatct
     3661 caggcgatac tcagcaatat ggatgtgagc gatgaagaat tatatactcc ggcgctctgg
     3721 cacggccccg cgggagcgta tcatatttca aaaaatctcg gaataaagga caacgatatt
     3781 ttaaacgcag tacgttatca cacagcaggc agagccgata tgtcgcttct tgaaaaaatc
     3841 gtttacttag gcgatctgac aagtgccgac agaagctata aggacgttga aaaataccgt
     3901 gaaatgagct ttgaaaatct tgacaacgct atgtacaatg cgttaaaata ttctataggc
     3961 gaaacgctcg gcaagggcgg agtaataccg ccgtgcacga tagcggggta taatttttac
     4021 accaagctca tgagagcaag aaaaagcgaa acagaaagga aaaatcctaa aaatgactga
     4081 tatagaaatt ctgaaagagg gcataaaggc tctcgactca aaaaaggcag atgatataaa
     4141 aatactcaag gtgcgcgacc ttacgatact tgcaaactat tttgtaattg caagcggttc
     4201 aagctcgacg caggtaaagg cacttgcaga tgaggtcgat ttcaagctcg gcgagaaggg
     4261 cgaagagcct aagtccattg acggcaaggc aagcggaaac tggatagttc tcgactacat
     4321 cgatgttgtt ttccacgttt tctataagga aacaagagat ttctatgacc ttgaaagact
     4381 ctggcaggac gctgaagagg ttgatatcaa ggagtttttg gactgaaaat cctgcatttg
     4441 agcaagaatt tttacgaaat tttcaaaaac gtattgacaa attcggtacg atattgtata
     4501 atactagcat acatctttat aaaatttgta tgagttataa cctgtgccat ttggcaaagg
     4561 gagggatata gatggcagaa gtacgtttag gcaaaaatga atctttagat acagctctca
     4621 agcgtttcaa gagatcatgc gccagagacg gcgtcctcgc agaggttcgt aaaagagaac
     4681 actacgaaaa accttcggta aagcgtaaga agaaatccga ggctgctcgt aagcgcaagt
     4741 ttaagtaatt atttacttac ggtaataaaa ggcggacaag gttagcctca tgctgacgct
     4801 ttgttcgtct ttttattttt gttatttttt gttaaaggat acggtatgat ttttaaaccg
     4861 cagatttgga taaattcagt tcttgacata gatgaggact ttcttgataa atataatata
     4921 aaagcactgg tgcttgatct tgataatacg ctgtcaatgc acggaaaccc tgtcgccgaa
     4981 agcggtgtac cggaatggct ccggcatatg aagagcatcg gcgtaccgat gcgtatagta
     5041 tcaaacaaca cgaataagcg cgtagcgccg ctcgcaaaga agctcggact tccgtttacc
     5101 gcaaacggct gtaagcccct tacattcggc atatcaaaag cgataaagat aatgggcgtg
     5161 ccgaaaaatc aggtagcagt agtcggagat cagatattta ccgatataat cgcaggaaat
     5221 atcaagggcg cggtatcggt gctggtcgag cctttccata tggagagcgc gtggacattc
     5281 agacttaaaa gaaaagccga gaacctgttt tttcacaggg attactcaaa gcttagatcc
     5341 aaggagaata agtaatggct gttaaattcg gaccgggcgg taattcgctg agcttcggca
     5401 agaagaaatt tcccgatgag ctgcccgcat atcttgcgtc aatggagctt gactgctatg
     5461 aggtcgagtg cggcagagga gtacgcattg cggcggcaac ctatgaaaag ctgcccgcac
     5521 ttgcccgtga taataatata accgtaacgc tccacgctcc gtacttcata tcgctttcaa
     5581 gcgttgaggc ggagaagcgt gacaacagcg tgaattatat tatggactca ctcagagccg
     5641 ccgacaagct cggagcgact aaggtcgtag tacattctgg ctcatgctca aagatgacaa
     5701 gggcagaagc gctgtacctt gcaaagcaga cgctttcaaa ggcggtcgcc gcacgaaaga
     5761 gcgagggact gaaggctatc atctgccccg aaactatggg taagataaat caactcggca
     5821 cacttgagga agtgatcgag ctgtgcacac ttgacgatga gatgctcccg tgtgtcgact
     5881 tcggtcacct taacgcgaga acgctcggtg cgataaagac tattgacgat tatgccgcga
     5941 tactcgacag cattgaaaac aagctcgggc acgacagact ttcgcagatg catatccact
     6001 tttcaaagat agaatatacc tccggcggtg aaaaacgtca tctgacgttt gaagacgagg
     6061 tttatggacc gcggtatgaa ccactgtgcg aactgttcgc gaagagaaac cttaactgca
     6121 cagtcatctg tgaatcggac ggaacacagg cggaggacgc atcaactatg aaaaaagctt
     6181 acctcggcta tctgaaataa ggacggaaaa taaaatgaaa atacttgtaa tgaacggacc
     6241 taatctaaac ctgctaggcg aacgtgaacc atcggtatac ggcgataatt cacttgcggc
     6301 aataaacgaa agacttaagg attgcgccga gaagaacggc gcacagatcg acttcttcca
     6361 gtcaaaccac gagggcgcgc tgatcgacgc tctccatgac gcaaggctta agtacgactg
     6421 cgttatcttc aatgccggcg catatacgca ctacagctac gctataaggg acgctatcgc
     6481 ggctataaag atacccgtaa tagaagtcca tatgtcaaat gttcacgcca gagaagagtt
     6541 cagacaccat tccgtgatat ctcctgtatg caagggtact atcgcgggct tcggtgaaaa
     6601 gagctatatg atggcgcttt tctacgcgct caacatggat tgattaagta tactggttga
     6661 ttttcggtta atatttaagt gttaaaatta tcttttgaaa aaatgcggtt tgccgcaacg
     6721 gaggaaatat ggttaagaat gcgggaaata tagcaaggct tgcggataat cttgcagatg
     6781 aaagacacgc ggcgcttata acgtcggata taagcagaag atatttcagt gggttcacat
     6841 catctgcggg tatgatactt gtaacaaaag aggcaagcta tctgcttatc gatttccgct
     6901 atttcgacaa ggctaaacaa cgtgttacag actgtgaagt agtgcttctt gaaaacgcaa
     6961 aaagccagct tatgagcctg ctgacaaagc acggcataac aactgtcagt atcgaaagca
     7021 gtgctatgac ggtaactgag cttgacagat acaaggcaaa ctactcgttc ataaagttcg
     7081 acagctcgcg tgagctgtcg gatatgattg agaaaatgcg tgttatcaaa acacccgaag
     7141 agatagagaa gatagtaaag gcgcagagaa tagcggaaaa ggctttttcc aggcttctgc
     7201 gcgacataaa gcccggacag acggaaaagc acgttgcagc actgcttgaa tactatatgg
     7261 cagagtacgg ctcagacggg aaatcgttcg ataccattgc ggcatcaggc gtaaattccg
     7321 cttcacctca tgcagtgcct accgataaaa agctcgaaaa cggannnnnn nnnncacttg
     7381 atttcggcgc gacctatgac ggctatcatt cggatatgac aagaacgata gctatcggca
     7441 aggtgacaga tgagatgaag agaatgtacg atgctgtgct tttcgcaaat cttgacgcta
     7501 tgaaggctat ccgtgccgat atcagcggca aggtggtaga cagtgtagca agaagtacgc
     7561 ttgatgcatg gggatacgga aagtatttcg gacatggact gggtcacggt gtaggacttg
     7621 agatacacga agcgcctacg gcttcaccgt catcgcagta tatgctcaaa gagggtatga
     7681 tactgacaga cgaacccggt gtgtatattt cgggtaaatt cggcgtaaga attgaagata
     7741 tggtggttgt gaccgaagac ggttgccgca atctaacgaa tacaacgaag gaacttatga
     7801 tcattaactg atacggaggg atacatatgc ttacaggctt gttttttgca atatcgggct
     7861 atctgctcgg aagtattctg tttgcaagca nnnnnnnnnn gggatttttg cctgtattta
     7921 tctatatgca cactgaaaat ccgtctgagg tcatgctccc tatagtgctt gttgcgccgg
     7981 taataggtca tatcctgcct attttttcaa agttcaaggg cggcaaggct atagctgtta
     8041 cattcggctc gcttgcgggt atgtggccta actggacaac ggtgctgacg ctgtgcttct
     8101 tctttatcct gtttatgacg ctgataaaga taacccccca ctgcttctgc actataattt
     8161 catattgctg tacgacgctt gtatcgttct tcatagagcc tgtcaaggcg gtatcggtag
     8221 gtatgctgat gatcaccatt gcctgctcga taaagatgct cagcgttccc gaagagcgcg
     8281 agaagttcga tgtcagatgg ctatggaagc actgatctta tcatgcagta
//
LOCUS       DAWAOG010000016         8040 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1328_length_8040_cov_2.512586, whole genome shotgun sequence.
ACCESSION   DAWAOG010000016 DAWAOG010000000
VERSION     DAWAOG010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8040)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8040
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1328_length_8040_cov_2.512586"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6074..6090
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7147..7156
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7664..7673
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2550 a         1458 c         2110 g         1885 t
ORIGIN      
        1 ccgctggtat gatcctgcgg actttgaagg gtttgacggg gaaaatatgt ttatcggaga
       61 atactatttt gccaatctag taagcaatat agaaagcgat aaaacgtatg acgatatata
      121 cagcgatatg acgcttgtga ccaaggacaa aaagataccg ggcggaacat acagaaagct
      181 gaataaggat tattacatag atgacgggta tttccttgtc tataatatat ccgggcaaaa
      241 caggagaatt attacgccgg acagcgactg ttccgaatat gatgagataa aagacgaaat
      301 aacaaacgcg tcggtaattg ataccgatgg tgcaaggctt gtaagatatt ccggcgaaag
      361 caacacgatg gcggaggata tattcggcaa ccgttttata ctgggcggtt cgtccgaaaa
      421 gaccggtatg tcggaatata catcctgtag tctgacggga aaagaatggc aaaaccaggt
      481 gatgatatat gacaacaggc tggagcccgt gcttacggtt tcttcgcgcg aaaatttcgg
      541 cgtaagggaa tatggcgata cgctgtatat ttcatcgtat aagtcaaagc ggcagaccga
      601 cgcaaaggtc aaatactatg cgctgatcga taagaacaac ggacacgttt ttgtgtatga
      661 cagcaaggtg ctgacaatgg taggaaatta tttttgcggt atcaagtcat cgggcattct
      721 gtcagactac tacacggctt acggaaaaaa gctcggcgag ttcatatacg caagagcata
      781 cggcgacaga ctgatgtgct ttgactatga taaatattat atctacgaca agtacggtca
      841 gctccttgca gagtatgaaa acaagccgag aataagcgaa aacagcggca gtgtggttgc
      901 gagaaacgca gacgaaagct tcacctgcta tgacagggat tttaatgtta tcctcaaaac
      961 aaagtacgac atacagccgc ttgaaaacgg atatatggct tactgcaatg ctcgcaacgg
     1021 caagatcggc tttatcgata aaaacggagg tgttgcgatt gaggcaaagg acgaatttgt
     1081 cagtgcaatg tcgccggacg gatattttgt gacaaagatc cgcagggatt ttgacgaaat
     1141 atatttggaa gtatacgcaa gaggtcttat tgatgtaatg atatatgacc gaaacggcaa
     1201 tgcggcaaca gaaaatatgg gcggaatgta caacgcggga aacagcgcaa caaacggata
     1261 ttcaccgctt gatttctatg cgggagcaaa gtactatgag aatacaccca ttatacggga
     1321 taatatacag tattacatag cagttgaccg aagcggcaaa gaagaccgat atatagatat
     1381 atacggaaat tatcattatg aggagtacgc agtaagcgat atcggcgatt cttatatgag
     1441 tgccggtata accgacaata cagctgtgac actcagcgtg cttatgtatg acgaaacggg
     1501 cagaaactca cggtcagtaa gctattatct taaccttgac ggcacgcctt ttataaaggg
     1561 cgacaggacg cttacacgac tgtcgggata tatcgcggga gaaagcgcag acggcaggta
     1621 caatatctat ggggaacacg gaaaggtgct tctcagcaat ataaaaatag agaaaagcaa
     1681 atatacagat tattggcaaa atgaaatata tattgaaaaa gcaggcaaca gtatgtcttt
     1741 taaagacgaa agcggcacaa gggtgtttga cctgacaaca caggaggtgc tgtttgaaga
     1801 tccgtacaga gatgtttact tgatagcggg aaatcttatt tattatataa taaacgacga
     1861 gtatacgaat gaaacggttt acagaaatct tgacaacggc aaagagtatc acactaatat
     1921 gtattcaata gattttatcg gcgttgatta tgaaaagccg caggaaagaa tagaatttcc
     1981 tttgaaatat gtaaagtgcg gagagaacgt atacggcgga actgatgctt atgaaacagg
     2041 ttatgattct ataagcgtgt atcagataga cgcaaatatg gaagagagcc ttgtgcatac
     2101 ctatacggcg ggtgagggcg agtgcatctt tgaactttac ggcgaaagag tgacggaagt
     2161 gtacggactg agaagtaact atgatgaaga gaacgaaacc gcgacctgga cggttttcaa
     2221 cgtaaagaac gataaaagcg taacagtgga taatgtaatt gaaatagaac gcttgcgtga
     2281 aaacaagatg tcgatatcca tatggaacgc cgagcatgag atagtaagat accaccttgg
     2341 aacagacgat atggtactta cggaagaaaa ttcgggagaa cagtgatatg agtagaatgt
     2401 gtccgaaatg taaacgtgaa atagacgaga atataaaatt ctgtccgttc tgtgcggcgt
     2461 ttacagggga taaaaacgat gaaaagaaga aaagccgcat aggcgttatt gtcggcagta
     2521 ttgcgggagc ggctgtgata tgcggcgcgg cggtcgcggt gtttgcgttt gatattttcg
     2581 gaatatttga gaatgagtcg gagacgcttt acggaagcgg cgagaagctg ttcaatatgt
     2641 cgctgtcgcc tgcgaagagc ggggataagt ggggatatat cggcaaggac ggaaagttta
     2701 agatagagcc gcagtttgac tgtgcttatc agtttgacga aaatgtaaac ggaaatgccg
     2761 ccgtcagcaa cgaaagcgga tacggcttta taaatgacaa gggtgagttt acggtcgagc
     2821 cgcgtttcag gcttgcgtca tattacagcg acaacggctt ttcggctgtg accgataatg
     2881 acggatatat gctgtatgtt gacagcaagg gcaggacggt gtatgacgag aagcatttta
     2941 catatgctca accctttgaa agcggagcgc cgtatacctt tgcatatacc gttatgacgc
     3001 aggacgctaa agatgataaa ggcgaaacgg tcggcgatat cacagatgaa tattatcttc
     3061 tgtcggcgga cggaaagacg gtgaccgggc ttcccgacag aatgggtata gactgcgtgt
     3121 tcggcgagta ctatgtcggg tttacaaaga acgtgaatga gggaagcaac gactttacca
     3181 aaagaagcta tgccgttttc tcggcagacg gtgagcagaa gagcgaatgg tgcgacagga
     3241 tattcgtgac ggacgagctt gtgctgatgt gcagaacgga caatgaaacg cggatatata
     3301 gagcggcagt atacgacaga gagctgaata agttaagcga tgagtattac tgcgacagaa
     3361 atcccgaatt tgccgacagc ggacttgtgc tgataaagcg tgacggtgag cgcttcagaa
     3421 aagtgcttgt gaataaaaac gaaggtaaac ttgacgaggt ttacactgca acagacggca
     3481 cggacataat aagcacattt gacaggagcg gcattgcttg cgtatacgaa aacggtttgt
     3541 actgcggtta tacggtcggc ggcaaggtct ttgagtgcga ctatgcgttt acggcgttca
     3601 acagcgggct tgcgccgtat tacgataacg caaagatcgg atatataaat gcgggcggcg
     3661 aggtcgtaat aaatgcgcag tatgatgcgg tgtcggagtt ctatgcggac gggtatgcct
     3721 atgtgcttgc taacggggaa tacagcatta tcgatactac cggtcagact gtagcgggaa
     3781 atctgagctt tgctccgaca aagctcacag acagtggtat aaaccacggt tggtatacac
     3841 cgaaagattt tgacggggca gagtattata aaaatgcaat aaattacggc acgttggcag
     3901 tgaagacgga cagcggcaac ccggactatt tttacgaaga ttatgtgaat aagcttgtat
     3961 atgcggctga cggagctgag gtaaaggaaa actccgaatt ggtgaagggt tacggcgagt
     4021 tcgaggacgg atatgccata ttggataaga acgggtatta tcttgttgac agccgggtaa
     4081 atgtgatacc gataccgtcg gacggaatgg ataagctgta tactacaaaa tatgcagacg
     4141 agtatttcta cggcgttgtg ggcagcggca gtaaggatcc gcttgctatg accgatgtca
     4201 acagaaacac gataacgctt tccgactatg acggagtgta tagcggcaat gactgcctgt
     4261 tcttaagcaa cagcaacagt gaaaacctgt atcacaaccg atacagggtg tataacggca
     4321 ggctttatcg accgctgaca atagaaacaa acaactacct gggatttaac ggaaatacga
     4381 agcatactgc aatgctgtca tgtacatatt acggtactgt tgatatgagc aatgatatcc
     4441 gccgcgttat tgacagcgaa accggagaat gcctgctcag ctataagcag gacggcggct
     4501 cgctcgaata tatgaatgaa tattttatct gtatcagctg tgatggaatg tcggactaca
     4561 gtgatgctga cggcaataca tatctggaaa agagcggaaa tgagtattat accgtatacg
     4621 gaaaaagaat aggtgtgttt atttcggctt ctgcctgtga tgataagctg ctggctgtaa
     4681 cagacaacgg aaaattcaga gtgtacagca ggtacggcga gctgctcggg gaatacggtt
     4741 atgcgtgcat gaacgaaaag tcgcagtata ttgttattga gcgggacgga aagtatatct
     4801 gccttgacag gagaatgaac gaggtgtttg acagcaagta cccattcgat ccgcctgtaa
     4861 acggctatgc ggcttatgcg gatatcaaaa gcggtaagat aggatatctc gatatgacgg
     4921 gcgaggtagt gatacctgcg tttacggagg tcgcggctga cttttcggct gacggatatg
     4981 caagggtcgc agaatacagg acaccggacg agtcggaaga ggcaggacgg tttaccgatg
     5041 aagtgctgat agacacaagc ggcaaaacag cttatgaaag caaaaataca gcggtcgggg
     5101 ctaacggctt tgcatatgac ggtgagtact tcaagactgt atattcgttt gcagggggct
     5161 atgagtattt tattaactac tgcaaggaga acaaagaaca gcttaagctg aacagctata
     5221 gcaacataat gcagtattgt catctgaaaa acgatggcag tattaccgac tatgcgggta
     5281 atgagccgta tcagttcgcg gtaatgaata agaagtcaaa attctctgcc gacatgtaca
     5341 gaatgtatga cggaacgccg atcgtgtata tttaccgctc aaggacgaat aatgaagtga
     5401 cgagttcata ttacggactg gacggaaaag agataaagtt tgacagatac ggtgacaatg
     5461 ggatttcact ggacggatat ttctggcagg agattcagga cgaagaaaca ggcaggaaaa
     5521 agctgtatat ctatgatact gacggcagtg aaaagttcaa tgtgactgca ccggagtatt
     5581 accattatgc cagtgcaaag gaagacggcg ggtatgtatt cgcattcaga tatcgttatg
     5641 atcctgatac tgagcttccg cccaaggtgt acaataatct tgttgcagac gcatattctt
     5701 ctgacgggaa gcttattgca cagtgtgaaa acgcttcgcc gtttgctacc ggcgctgttg
     5761 agggaaacaa ggacgagata cgctttgtct gccggacgga agatgaaaag tatgaggaaa
     5821 gaatatactc cgtatcgaaa ggtgaattta tcagtacaaa gcaggtcgaa atcggcgatt
     5881 atgtttatgc atagaaatga gcggcaactg atctgtgaca gcgatgctat gcgataaaaa
     5941 ccgcttgaca ggtaacaggc gttgtgatac acttaaaaca gttaagcgta aggatacagg
     6001 cttattatcc gtgggagata tattgatatc aagggcggtt acatattgat atcaggcaca
     6061 gctcacggtg ggcnnnnnnn nnnnnnnnnn tgcctttttg cgtctgagat cgaaatataa
     6121 ggagaaggtt ttatgaaaat gaaaaagtgg atgttggggc ttataacctt tgcggcgatg
     6181 gttgtgatct gtgcggtgtg cgcgggagcg gaaaatgaga atatggcata tatcgtgtat
     6241 gccgacagcg attggtatcc cttcgtattg gacggttcgg gatatacaga tagcggtata
     6301 ggaacggtta caacatggaa agccggaaac tgcgatattt ctgaaagcgg aacatatact
     6361 gtttctgtat ccgatatcaa aatttcgaca attgacgagg aaacaggcga agaaattatg
     6421 gagcccgtca acggagaagg tgctgttgta cttgtagtcg atatccaaga tatcagtgct
     6481 gtccttggtc tgggcaaagg cagcgaaggc tttgacagaa ttgcgacaag caagggtcgc
     6541 atggattttg caaagagtaa aggtgctgat gtaacagata tcaaaataac acaggtcaaa
     6601 gacggaaaag caactgatat tccggtagat attagtaaga tctacttcgg ggatattgaa
     6661 gacaacggaa aattcagaat agaaatatac agcgattacg gcgatactaa gtacgacccg
     6721 ccgatagaca ccgagaaaat cgactttaac gaaagtcttt cagttcagtt tacaattgtt
     6781 ttgccgaagg aaattcaaaa acctacatat gaatacgccg tacttccgga tgaaacaatt
     6841 gaaataatcg gatacaacgg aagtgaaagt aaagcagtta tccctgataa aatcgatggg
     6901 aagaaagtca caggtatagg cgattatgct tttgaaagtt gcgcaaatct tacaagtata
     6961 acgatacctg atagcgtgac gaatataggc gaatacgctt ttcaaagctg ctcaaatctt
     7021 acaagcataa caataccgga cagtgtaaca aatataggac aaagagcatt cttgttctgc
     7081 aaaagcatca aaaatataac gataccgaaa agcgtgcagg atatcggcga gtatgcgttc
     7141 cacagcnnnn nnnnnnctgt acaagtcttg aaaaaataaa tgttgcgaca ggcaatgaaa
     7201 attacgtttc tgtaaacgga attcttttca gcaaggataa aacaaaaatc gtatgctatc
     7261 ccgccaacaa gagcggtaca agttatagca tacctaacag cgttaaggtt attggcagtg
     7321 ccgcattccg tgattgctca catcttaaaa gtataacgat acccgacagc gtttcaacta
     7381 ttgaacatca cgcttttagt tgctgtacaa gccttacaaa ggttacaata cctgacggtg
     7441 ttacgatcat tgaaatgggc gtcttcgaca actgcagaag tcttacaagt gtaaggatac
     7501 ccgacagcgt gacagaaata ggacacagtg cttttaatgc ctgcatcagc cttaaaaata
     7561 taactgtacc ccgcagtgta acaaaaatag gcttctttgg cttaggagct gtcggaacgg
     7621 aaaacaaggt agccgacctt acgatctact gctatagtgg cacnnnnnnn nnnaagttgc
     7681 ctacgcttgc taaggtaaac ggagttaagc tcggcggcag ggcggctgac gcgctgagaa
     7741 taaactggac taaaaacaca aatgctgacg gatacatcgt tgagatgtat cagggcgaca
     7801 agtgggtaag agtcgctaag attacgaata acagtacaac tactttcagg aaggcagggc
     7861 ttaaggcagg cactgtttac aagttcagag ttaaggcata caagatgtat gacattactg
     7921 cggcttacag tgcatacagc gctacagtta ccgcaaggac aaatccgtct gttataaagg
     7981 gcgctaagct cggcggcagg gcggctgacg cgctccgtat caactggact aagaatacaa
//
LOCUS       DAWAOG010000017         7882 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1350_length_7882_cov_2.163025, whole genome shotgun sequence.
ACCESSION   DAWAOG010000017 DAWAOG010000000
VERSION     DAWAOG010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7882)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1350_length_7882_cov_2.163025"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3058..3067
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1916 a         1932 c         1709 g         2315 t
ORIGIN      
        1 gcccataata aagtaggcga ttaccgcttt tgtgccgtct gcggattttc ctatcacgat
       61 gcctctgccg tgataagcaa gagagttaag ctctttttca agtgttacaa cttccatata
      121 gcctcctgtt gattacagcg tgccgagata gcacaccgtc tgatataaca cggtcacatg
      181 accgtccgtt acaaattcgt caaacgcttt ttcaagctcc gataaaaatt ccctgtaatt
      241 gccgtcactg cttttcggtg cgtgtgaacg tgacagcgta tctccgataa actgttcctt
      301 tgtcataaga aacgggttat cgtatgtgaa aaccttgcag tcgcccaaat atacttcttt
      361 gaagaatttg tcgccctctt tttcctcatc aacgccgtta tgtccgacaa agacgtgccc
      421 cgtgtggaat gtttcgcagt accttgtaaa gatctcgtca cgccttgccc aaaaatcatt
      481 ccgcacacgt ctgttccata taacagcaag ttttccgttc ggtttcagta tacgtttaca
      541 ttcatttctg aacatatcct tatcaaacca gtgaaatgcc tgcgctgtgg tgattaggtc
      601 aaaagaactt tgcggcaatc ctgtgttttc ggcactgttt tgtacagccg tgatatccgg
      661 cagatttgct ctcagcaccg aatacatatc gccgtttggc tcaactgctg ttacattgcg
      721 gaattttccg agaagtgcct ttgtaaatat gcccgttcct gcgccgatat cggcaacacg
      781 gctgttttca tctccgtgta tttcattata aagaacgtta atcagtgccg tagggtatgt
      841 gggacggtac ttgtcgtata tttctgcttt accgctgaac ttttcttcgt tcattattcc
      901 tccactatag ttatgccgtc tttcacggtt atctttccct cgcagaaaag acgcgcggca
      961 tgggggagta tcttccactc cgcctgctcc ataacacgtt tctgaagaat ttcgggggtg
     1021 tcgccgtttt tgacctcaac tgccttttgc agaatgatag gtcctgcgtc acattccgct
     1081 gttacaaaat gaacggtagc gcctgttacc ttcacgccct ttttaagcgc ttcctcgtga
     1141 acgtgaagtc catagtagcc ctttccgcag aaagagggga taagggcagg gtgaacattt
     1201 atcatcttat acttgaacgc atcgcatacc tgcttgtcaa gtattgtcat aaagcctgcg
     1261 tagataacaa ggtcagcctg ttcttctttt aaagcgtcag ccatagcctt gctgtaagcg
     1321 gcaacatcgg cataatcacg ccttgcgagc actcttgtct ttatgccgtt atctgcggct
     1381 cttgtcagtg catatgcatc gggctttgac gcgataacgc aggtgatctt acctccgccg
     1441 agcccttcgg acttttcggc atcgataagt gcctgcaggt tagttccgcc gcccgataca
     1501 agtacaacga tatttttcat attactcaat tatgatgccc tcgtcaccct tgattatttc
     1561 accgatacag tagctttcaa cacctgcatt tctgagtatc tgtacagctt catcggcgtc
     1621 gtccttgtca actacaacgg tcatgccgat acccatattg aaagtattgt acatatcgtg
     1681 ttcggagatg tttccgactt cctgtaagtg cttgaagatg taaggtactt catagctgtg
     1741 ctttgtgatc tttgctgtga agccgtcagg gagtgaacgc ggtacgttct cgttaaagcc
     1801 gccgcctgtg atatgcgaga tagcctttac ctgcttcttt gagataagct ctaatatggg
     1861 cttaacgtag atctttgtag gtgtaaggag tgtttcaccg agagtttttc ctgtggggag
     1921 gacttcatcg agaacttcct tgctgtttat gttgaacacc tttcttacaa gagagaagcc
     1981 gtttgaatga acgcctgtgg aagcaaggcc gattatgatg tcgccttcct ttacggtgct
     2041 gttgtcgatt atctttgact tgtcaacgac acctgttgaa tagcctgcaa tgtcgtattc
     2101 gtcctcgggc atcataccgg ggtgctctgc ggtttcgccg cctataagcg cacagcctga
     2161 ctgtacacag ccgtctgcaa caccttttac tatagcggct accttttcag gtacgttctt
     2221 gcctatagcg atatagtcaa ggaagtagag gggctttgca ccgcagcaga ttatatcgtt
     2281 tacgcacata gcaactgcat ctataccgat agtatcatgc ttgtccatca gcatagcaag
     2341 ctttagcttt gtgccgacac cgtctgtacc cgaaacgagt acgggctcct tgatatccga
     2401 aacatcaagc tggaacagac cgccgaaacc gccgatacct gtaagtacac cggggatatt
     2461 ggtgcgttca acgtcctttt tcatcagacg aacgccctcg tagcctgcgg taacgtcaac
     2521 gcctgctgcc ttgtagcttt ctgaaaaact gttcttcata ataataattc cttccgtttg
     2581 gggacttttg tccttatgtg gtggcttctg atttgctttc gcttattttt ctgtcaaatt
     2641 tatccttata catttctgtc gggatatccg taggatattc tccgctgaag caagccgtgc
     2701 aatagccgca ctcacagccg tttgcatgta ccgcaagctt ctttgcgctt tcaacgctca
     2761 ggtatccgag tgaatttgta ccgattatat cgcttatctc ttcaaccgtg tggtgacaag
     2821 caatgagatg atcccttgaa tcaatatccg tgccgtaata gcatgggttc agaaacggcg
     2881 gagcggaaac tctcatatgg acttccgtag cgcccgcctc acgcagtaat tttacaattc
     2941 tgccgcttgt agtgcctctt acaatagagt cgtcaatcat tataactctt tttccgtcaa
     3001 ccgcttcgcg cattgcgttt aattttatcc tcagtgcaga ctcacgttct tcctgagnnn
     3061 nnnnnnnata gagtcgtcaa tcagcaccac acgcttgccc tttaccacgc ttgctactac
     3121 attaagcttt atccttacct tgtcctctct tgacgattgt ccgggagaga taaaggtcct
     3181 gccgatgtac ttgtttttga taaagccgat accgtagggt atacccgact ggtgagaata
     3241 gcctatggcg gcatctattc ccgagtcggg aacgccgatc actatatcag cctgcaccgg
     3301 gtgttcaagc gctaagaaag cacctgcacg cagtcttgcc tcgtgcaccg atgcaccctc
     3361 gatgaccgag tccggacggg caaagtagat atattcaaaa acgcacattg aatgcggacg
     3421 cttgccgcaa tggtctttta tagagcgtac tccgctctcg tcaaatacaa cgatctcgcc
     3481 ggggagaaga tcccttacga actttgcacc tactgagtca agcgcacagc tttcggaggc
     3541 tactatatat gttccctcct cggtcacacc gtaacaaagc ggtctgaaac cgttttcatc
     3601 acgggcggcg ataagctttg cgggtgacat gataaccagc gaatatgcac cgtttatcct
     3661 gttcatggca cggttgagag cctcttctat agacggggct tcaagccttt cctttgttat
     3721 aaggtagctt attacctctg tgtcgcttgt agtgtggaag atcgagccct gcttttcaag
     3781 ctcgtggcga agctcataag tatttacaag gttgccgttg tgagcaagcg ccattcttcc
     3841 tttatagtga tttacgagga taggctgagc attaaggcgg gcattggctc cggtagtgcc
     3901 gtaacgcaca tgacctactg ccatattacc ctgtcccagt ccgtcaagcc gctcgggagt
     3961 gaacacatcg ttcacaagtc ccgtatcctt gtaggagttg aacacaccgt catcgttcac
     4021 gacaatgccg cagctttcct gtcctctgtg ctgtaatgca aatagtccgt aatatgtggt
     4081 tgcggctaca ttggtctttt cttttgcaaa aacaccgaac acaccgcact cttcgtgcaa
     4141 accgtttgtc aatggagtca ttcctttccg ttccagcaat aagtgcaaag ctcacactgt
     4201 ggcttgccta ttgcccgtac agtaccctca agcgactgga actcaagcga tgtcagcttt
     4261 aatctgcgac atatctcctc gcggagcttc ctgcctctct ctgtgttggt gtcactgtac
     4321 tcgtcgataa atctgatacc atctgcaccc tcggcctcat caattatctc catctccgat
     4381 gtactgcggg agaagttgag atacttgcaa ccgtacatta tcggaggaca ggcactgcgc
     4441 atatgtactt ccttagcacc gttctcgtag agaaactcta ccgtttctct catctgtgtt
     4501 cctcttacta tgctgtcgtc aacgaacagc agcttcttgc cctcgataag ctcatggacc
     4561 gggataagct tcatcttagc gacctgattt ctcattgtct ggttggtcgg cataaagctt
     4621 cttggccatg tgggggtata ttttatgaaa ggacgagcaa acgggatacc gcttctgttt
     4681 gcgtagccta ttgcgtgagg tacgcccgaa tccggaatac cgcatacata gtcaacgtcc
     4741 ggcagcttac cgttctttat atcgttctct gccatgatct cgccgttgcg ggttcgcatc
     4801 gtttcaacgg tcacaccctc atataccgca ttcggatagc cgtaatatgt ccacatgaac
     4861 gagcatatat gcatcttgct ttcatcgccg tcgctgagca cctcacagcc gtccgcggtt
     4921 atcatatcta tctcgccggg acgaagctct ttatatgtgc tgtagccgag cttctggaac
     4981 gcgaacgatt cgagcgatac gcagtagccg tcacttcttt ttcctacaat gataggaagt
     5041 ctgccgtact gatcccttgc ggcgataatt ccgtcctttg tgagaatgat aagtgacatc
     5101 gtaccgtcga tcttgctctg ggcatacctt atgccatcgg taaaattgtc cttctgtgcg
     5161 ataagcgcac cgatgagcga gcctgagttg atacttccgc tgctcattgt ttcaaagttc
     5221 gcacagccgc tctcgagcag ctcggcagaa agctgttcag cgtttctgat aatgccgaca
     5281 ctgcatattg agtaaattcc gagctttgaa cgcaccagaa tgggctgagg gtcggtatcg
     5341 ctgatacagc cgatacacag ctttcctctc atctccgtag cgtcctgttc aaacttcgtt
     5401 ctgaacggtg agttttcaat actgtgtata gcacgctgaa agcctctgtc ggggctgtat
     5461 gcggtcatac cgcctctgcg tgttccgagg tgggagtgat agtctgttcc gaagaaaaca
     5521 tcggttatgc agtcgttgtt tgaagctgca ccgaaaaatc cacccatata ttatcctgcc
     5581 ttttcctata gattatttta ccggaagtga tcattcaccc ataagacgct tcatgatctc
     5641 tgcgtaagcg tcctcaacgc cgcccatatc gcgacggaaa cgatccttgt caagcttttc
     5701 gtgagttgtg ctgtcccaga aacggcaagt atcgggagag atctcatcag cgaggattat
     5761 tgtaccgtcc gctgtcttgc caaattcgat cttgaagtcg ataaggtcaa tgttgagctt
     5821 cttgaagaag tcaagcataa actcgtttac cttaaaagcg tactttgaga taaggtcgat
     5881 ctcttccttt gttgcaagac cgagtgcgag tgcatagtag tcgttgatga aaggatcgcc
     5941 cagatcatca ttcttgtagc tgaattcaag cgtggggcag aggagctttg tgccttcctc
     6001 aatgccgagc ttctttgaga agcttcctgc ggcaacgttt cttacgatta cttcgagggg
     6061 aactatagaa accttcttta cgagcgtttc tctgtcgtta agctcttcaa caagatgagt
     6121 cggaactcct gccttttcaa gaagcttaaa catatagttt gtcattctgt tattgataac
     6181 gcccttaccg gagatagtgc ctttcttaag gccgttgaat gccgttgcat cgtccttata
     6241 ggatacgata acgagattgg ggtcgtctgt cgcgaaaacc ttctttgctt ttccttcgta
     6301 gatctgttct ttcttttcca ttgtgtttat cctttccttc acacggctat gccgtaattt
     6361 tgttaaatcc tattatatat agtacaccga tcaaagctcg gcaagtacct gctccatttt
     6421 cttgtcggct tcaagaacct ttgcggtctg tgccgaacgg tatgctttca tcttgtccat
     6481 aagtgcatcg tcagataccg caagtatctg tacagcgagg aatgccgcat ttgcggcgcc
     6541 gtctatagct actgtagcta ccggcacgcc tgcgggcatc tgaactgttg acagaagtgc
     6601 gtcaataccc tcaagagcac ttgcttttat cggaatgccg ataacgggca ggggagtctg
     6661 tcctgcgata gcaccggcaa gatgagccgc tttgcctgct gcggcgatta taacgcctgt
     6721 gccgttcttt tcggcgtttc ttgcaaactc ggatacctct gcggcacaac ggtgagcgga
     6781 gagaacacga agctctgtag gaactccgaa gtccttaagc acctgagccg cttttttaac
     6841 gataggcaga tcagaatcgc tgcccattac tatggctact tttttcattt acgaacatcc
     6901 tttcagaaaa taaataaagc tgtgtaatca cattgcacag cttaaaaaat acacttattc
     6961 ttataatacg gcgtagaaaa agacatatat gaaaatttac cgaggcttat caaagctgtt
     7021 gtaagctgta ttttattcat agatctcatg ctttccctcc gtacttgtta ctatatcatt
     7081 ttaccttatt ttaataacaa attcaagtgc taaagcggaa aatctctcaa attgtgcttt
     7141 ttcaacaatt atctcatttt gtttttgtgc tatatgcaca tttattactt aataaacttt
     7201 ttgtgagtta atttagcgta aaatcgttta catctcgtta caaaatgaaa aatatcgatt
     7261 gtaatgcgaa aaatcagcaa aatcagtaat tgagttgaaa ctgatttgtt acaaagttgt
     7321 aactgaattt tgtgcaaaat cacgaaaaaa acttcaaaaa acgatttttg taaacgttta
     7381 cggtaaaaaa acacaacttc tctcccaaaa cgcttgcaaa tttgtctaat gtgtgatatc
     7441 attatgtcag cgaggaaaaa gtaaacaaaa cttttgatga gtatacgcgg tttttagtga
     7501 attatccatt gttctatatg aagttacata taacgccata taacagggag cttcaactga
     7561 aacggtacat tctcaaggcg ggttacactt cctcttaaac gcgaccgaaa ggtcaggcag
     7621 tgtttatagc aaagactata ttacacaaaa acgaatactt ctggaggaat taccaatgaa
     7681 gaaaagaatt ttagcagctg tattagcatc tgcatcaatt ctcggcgcag tagcaggctg
     7741 ctcaagcaac aacgctacaa gctcaacagg atcttctaca acagattcta gcaagactga
     7801 agactcaagc aagactgagg attcaagcaa gactgacgac agcaagactg aggcaacaga
     7861 gctcgaggat gacggcgaca ag
//
LOCUS       DAWAOG010000018         7766 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1368_length_7766_cov_2.396965, whole genome shotgun sequence.
ACCESSION   DAWAOG010000018 DAWAOG010000000
VERSION     DAWAOG010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 7766)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7766)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7766
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1368_length_7766_cov_2.396965"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3068..3077
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6549..6558
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7518..7533
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1985 a         2033 c         1563 g         2149 t
ORIGIN      
        1 gctcaacaag gctcagatcg cccggcatta agtccttgag cagatttctg tccgcttctt
       61 ctatactttt ttcgcttgta ctgttttcgt tatcggcgtt tccgttttca tgtgcatata
      121 caggtatgct tgttgagagt aatgagatcg ccaatagtaa aacagtgaat aaatgttttt
      181 ttcttttcat aagcacctcc gttttgtttt tgaaaaagaa acactttttc tattgttaga
      241 atatcattta tgctatgcaa aatcaaggct tgtcttgata ttttgtgaaa tatagccaac
      301 aaaaattgtc aaaataacca aacagcgact tgccctcgca agccgccgct tctcatctca
      361 cttaacctca ataacaacat ctgcacctat tcccgccgcc tttattactc cgcttttctc
      421 tgcggtgtcg ggctcatacg gttttccgtc ccttatataa gctataccgc actttctgcc
      481 gttaccgaga atatcgggga tattctccgc cgggatatgc acctttgcgg tcgctctcca
      541 caggttgata tcaagcactc cgtttatcct gtcagccgtt atgtccatat cggactttga
      601 ggtaagctct atgcttcccg agctgtcctc tataacaacg ctgtccgcct ccccgtcata
      661 ttcaagccgc cttatatcaa gcgagctcac acacagcagt cttgcctgtg cggatatttc
      721 acagcagtcg gtaaactgct tcggtagcag tatcttcacc gatacattct cctgcgcttc
      781 atacctgctc atcttatcct tgcgcaggca ctctacatca agcttgtttt tacggctgtc
      841 gagccttacc ttgaataccg agccgatatc ctccgccgtt tccgaagaga atataacgtg
      901 caacttctca tcatcaccgc cggatacggt gatatcacgc acgcttccgg catttatatc
      961 aaagtgtgtg ttgacatcta tatcatatgc cgtttcgctc gtgtactgag ccgcaccgcc
     1021 tttttccggc aaatttccgc ttacaagctc gtcaagtgtc accttgaata tctccgcaag
     1081 tatagtcata ttttcgatat ctggaagacc ctttccggtt tcccactttg tcaccgcctg
     1141 acgtgaaacg cctatacgct ccgcaagctc ttcctgcgag atgccctcgc tctttcttat
     1201 ctgccttagc ttttcactga aattcatata tgtttcctcc ttgatttgtc gtgtttttga
     1261 acagcccgat tataaccctt tttccgcagt agccgcaata aacatctgct tacaatcgtt
     1321 atttccgggc gctactttgc gttgcatacg aaaaatcggc atagcaaagc ggaatgtgaa
     1381 gccacattcc gccgtgatct ttattaaaac tgcaacagca tcacaaccat tgccaaaata
     1441 aacaccggtg aaagcaccgc caacatcttc ttatgcttat gctctcctcc gacaggcgta
     1501 agctttgccg ctccgcagaa cgcaagcaat accgccacaa acggcgccgc aaagtaggag
     1561 atgttcacga taatgtttcc gagcgccgcc gccgcatcta caagcccgtc ccacaatccg
     1621 ccgatgccag tgagctgctt ctgatacgtt atgaccgtca caaagaagtt gtttatcagg
     1681 tgcagtacca ttccggggat tatcgtgtcg tatcttaccg ccataagtcc aaatattata
     1741 ccgcttaaaa acgcatatgc aaactggtca aaattgccgt gcgccagtcc gaacaccagc
     1801 gccgatataa ttatcgcagg cgtatcgcct ataggcttaa gcgagcgcaa cagcagatga
     1861 cgatagatta tctcctcaaa tatcggcgca acaaacgccg taaagataag cgttaccacg
     1921 cccgcggaaa aactcgacgg agctattgct tccatcacat tctgtatctc tcccgcaccg
     1981 aacagcagct gtaatatata atttatacag gtcgttatat tgcttcccca cattgcaaac
     2041 gcaaacatag caggtatgaa tataagcgga tagtacgact tgttctcata ctgcgtgtca
     2101 agacgcttca gcggtctgta cttcttatac agcaccccga acgcaagcag cttcgggata
     2161 tatgtcgata tattgctgaa agtgtaatta agcacagtaa acatagtact gcctgtttca
     2221 agcccggcat tcacaaagcc cctcagcagg aacaccatcg caatgaatat aatcttttgc
     2281 agtaaaacgg atacgatcag cgcaactccg acatcaaggc atatcctgcg tatatccgca
     2341 ggcttaaggt cgtctttcgt ggtcagaata tcaaagctct gctgtctgta cctgtctgca
     2401 ataataccgg taaggctttt ctgcatttta acagagtcct ttcgttaaat aatcatcata
     2461 gctattatat acgccgtgca cagaataaat cccgcaataa acaaaggttc ttttgccaca
     2521 gcgatgaatt tctgcttttt ggtaagtgcg ggatagttgt ttaccggtcg gtaaaggcga
     2581 ttgttcttaa gctttcctat agccgccgca atgccgacta cagctattat cagtataaca
     2641 gcaaggtaaa tcatcagcgc tgtcataaac gcctgttcgc cgcctgtaag cccctcgccc
     2701 tgctgaatat gcgtaaacgc cgatataaaa gtttcactgc ttacagccac gatcgctatt
     2761 cctgcaatag agttgaatat cgcgtgcatt atcattgtcg gcacaagact gccggtcgcg
     2821 tatctgacat agcccagaca tatgcctact atcaccgtat agcagaactg atgtatattg
     2881 ccgtgcgcca ttccgaagca cagcgatgaa acgagtatcg caaagccgtt tccgtagggc
     2941 ataagcgagg actgtataac tcccctgaac cagtattcct caaacaccgg tgccagcacc
     3001 gccatttgta taaacagaaa agcaagatac cagccgccaa gcccaagccc ctttgacact
     3061 atcggtgnnn nnnnnnnatc ctgtactata aaaaaagata tcgccatcac cgcaatgttt
     3121 gccacctgac ccgctccgta cattggtacg aaccacgata cagccttgcc gagccttttg
     3181 ggcttcttgt aaagcctgcc gtcaaagtga tatccgagca gctttgatgt gaccgctatc
     3241 gccagcacat acagtataag tatacccgcg agcgcctgca tcgaagacgt tatctgcgcg
     3301 tcgcagtcag ccataagcgc cccgatgagc gcagttaccg catcagccgc acaccgtgcc
     3361 gcgaatatga ctgttaaagc cagtcccgcc tttttgcagg ctgtcctgaa atcagcggcg
     3421 cacatcgaga acttctcccg tttcgagcat ttcctttaca tagctcggga gcgcaaagca
     3481 gcccttgtgg agctccgtgt tatagtatct tgtcttaaga ccgagggagt tccagtgctt
     3541 tgcatcaagg tcgtttatcg gatcaagccc cttacttgca aagccgaaca gccagtgacc
     3601 actggcatac gtcgggatat gagcctgata aacagcgcat accgggaaca ccgattttat
     3661 gcgctcgtgc gccctgcaca tagcctttgc gtcattaaca tagaacgggc tttcatgctg
     3721 atttacaagc acgcccgtgt ctgtcagcgc attatagcag tttccgtaaa actcaggcgt
     3781 gaaaagaccc tctcccggtc cgaacgggtc ggtagaatca actattataa ggtcgtattc
     3841 cccctgctta tgtcttacat agcgcagtcc gtccgtaaaa tgcatatcaa gtctggggtc
     3901 gtgcatagag accgtcatct cggggaaata ctcctcgcag acctcaacca cctttttatc
     3961 tatctcgaca aggtcgatat gctctatcgt gctgtaacgg cacagctctc ttaccgtgcc
     4021 gccgtcgccc gcaccgataa caagtacgtt ctttatatcc ttctttaccg ccatcggaac
     4081 gtgtactatc atatcatgat agataaattc gtccttctgc gttatcatca catatccgtc
     4141 aagcgcaagc agtctgccaa attcaagcga ctgaaaaacg tctatcctct gtacagctgt
     4201 ttgtctactc ttatcgaaaa cttaacactg tcgctgtgat tttcagtaaa ccataaatcc
     4261 atattattac cgtcctttca atctgtaaac gcataagtct atgtggtacg gcgggcgatc
     4321 ttaatcgtcc agtatcataa tgttctttat ttcaaggtcc tgtgtacccg taagaaaaca
     4381 gcccttatcc cccgcatact ttatatatgc gagaatttca tcggttatct gctctccggg
     4441 cgcaagtatc ggtattccgg gcggatagca cattacaaat tcgccgctta tcttacccga
     4501 actgcgctct atcggcacac tcttcttgtt tccatagaat gcgtcctgag gcgacagctt
     4561 tactatagga ttgatatatt cgtgatcaag catacccgtc ttatcctttg aatagatacg
     4621 ctttatgtca ttgagcgcac cgataagcct gtcaagatac agctccctgt cgcctatcga
     4681 aacataagcg aggatatttc ctatatcgcc gaattctatc tgtattccgt agcagtcacg
     4741 gagaatatca tacacctcaa tgcccgcaag tccgatatct cttgtgtgta tcgacagctt
     4801 tgtaacatcg aaatcgtaga acgctcctcc gtcacacagc tccttcgaga atgcgtaata
     4861 gccgcctatc tcgtttatct catcacgcat atactgcgca tatgactgca cctttgcgaa
     4921 tatctccttg ccgtgaagcg caaggtttct tctcgatata tcaaggctcg acatcagaag
     4981 atagcttccg cttgtcgtct gcataaggtt tattatctgc cttacatagc cctcgttcac
     5041 tgtgtccgct gtaagcaaga agctgctctg cgtcagcgag ccgccgctct tgtgcatact
     5101 tactgcggca aagtccgcgc ccgctttcat tcctgcaagc ggcatattgt cgccaaagta
     5161 gaaatgcgtt ccgtgcgcct cgtccgcaag caagtacatt ccgtgagcgt gtgcaagatc
     5221 cgcaatacgc tttatatccg agcatactcc gtaataggtc gggttattta caaataccgc
     5281 cttggcatcg ggattttcaa gtatagcctt ttcaacatcc tcgaccgtca ttccgagcgg
     5341 tatgcccagc tccttgttta ctccggggtt tacatatacc gtcatgcatt ccctgcctaa
     5401 aaattccgtc agctcggggt tgcccctgcc catcttatga ccgggtacgt caaaaggcac
     5461 tatacgcttt gcccggtatt cctttaacgc ctcatatata ggcgcgcgct cctgtgagag
     5521 catatcatca gtcctttcgg aaaggaagta atgttgtcat agtatcacat tgatattttt
     5581 gcaggggagg ggcttgtccc tcccgcaaat ttattgatta cgtcttttgt cgatttatag
     5641 aacgaagtgt gaagttcact cacccgtaaa tattcattcc cgaatatatc tcgatcatct
     5701 ctcgccttaa gttctccgta attctcagtc gctccttagg cgggatatca tatatatccg
     5761 tattgaacag gtagtttttc agatccatct ctcgcaccat aagtcttgtg tggaatatgt
     5821 tcgcctgata cacgtttata tcggttgcgt catattttgt cagcgtataa tcgttgataa
     5881 aatcctggat cgaagttata tcgtggtcga gatacagctt tcttccgcct acatcacggg
     5941 taaagcctct caccctgtaa tctatcgtta tgatatcgga atcgaaactg ccgataaggt
     6001 aatccagcgc attaagcggc gagatctctc cgcaggtcga aacgtcaata tctaccctga
     6061 atgtagaaat actgttgttg gggtgatatt cggggtaagt gtgcaccgta acgtgcgact
     6121 tgtcaagatg cgccaccacc gtttcgggag ctgctgccgc accgcgctga gggtgaagcg
     6181 gctttccgtc caccattccc ctgttgcatg acttatctat ctccatgctg tcggttgcct
     6241 tttcacttat cagcagtgtt acgctcgcgc cctgcggatc gtagtcctgc tttgaaatat
     6301 taagcacatt cgccccgatt atatccgtaa catcacacaa tatacccgtc agacgctctg
     6361 agttatactg ctcatcgata tagtcaatat attctttttg ttctcttgcc cctttagcat
     6421 aacaaacatc ataaatgtta aagctgaggg tctttgtgag gttgttgaaa ccgtaaagtt
     6481 taagcttttt ttccaacaga cacacaacct ttcggaaaaa tatctgcata taaacacagc
     6541 tactataann nnnnnnnnaa agcatataag ttcattaaaa agccagccgc cgacggcttt
     6601 gatgctcatc ggcggctctg aatattattc tgttttaatt tgagattaca cgttatcctg
     6661 accgggaaca tactcaagac tgtcgtctga agttgtaacc tctgtagctt ccttagactt
     6721 tatttctccc gaagcatcat tcttatcctt gaatacaaac ttcagcagga taggagttac
     6781 tacagtagta ataacgacca taattactat aggaccgaaa agtccgctgc tcataagtcc
     6841 catattaagg cccttatccg caactatcag cgcaacctcg ccacgggaaa tcattcccac
     6901 gccgatttgc aaagcctctt tgtttgtgta tctgcatatc ttcgcaccaa gtccgcagcc
     6961 gacaaccttt gacagtatag cgactaccag cagtacaacg gcaaatatga tgattgtagg
     7021 tgtcatattt gtaagcgtta ccttaagacc tatgcttgcg aagaagatag gcgataacag
     7081 cagataagac atcgtgtcaa atctgtgtgc gatatacttt gtctttgacg tacttgaaat
     7141 tacaagacct gcaacgaacg aacctgtaat atcggcaacg ccgaaaaatt cttccgcaac
     7201 atacgcaaag agaaggcaga gcgcgaagct tacaataaca taacggcgct gattttcttc
     7261 cgttctctgc gtccacttgt tgaacacgaa gtggaagaat ataccgaaca gcaaagcaaa
     7321 tgcaaagaaa ccgagtatct tcagtattac tatacctatg tttcccgatt cggggtctgc
     7381 aagcgatgtg attacagtca gcgcaatgat accgagtaca tcatctataa gagccgcacc
     7441 gagtatcgcg ttacctgctc ttgtgttgag ttttccgagt tctttaagcg tttcaacagt
     7501 gatacttacc gatgttgnnn nnnnnnnnnn nnncctgtaa gaatgttgat gtcgaaagag
     7561 tgggatctgc cgtaccgaac aggaacgctg tagcggcacc gccgaccaga ggaacgataa
     7621 caccgataag agctattatc gagcttgcaa gtccgcactt tttcatctcg cggatatccg
     7681 tttcaagacc tgcgacgaac ataagcacga taacgcctat ctccgacaga ttgctgagta
     7741 tcggagcgtt gtcaagagat acaagg
//
LOCUS       DAWAOG010000019         7704 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1381_length_7704_cov_2.485161, whole genome shotgun sequence.
ACCESSION   DAWAOG010000019 DAWAOG010000000
VERSION     DAWAOG010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1381_length_7704_cov_2.485161"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2663..2672
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6888..6897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1990 a         1813 c         1565 g         2316 t
ORIGIN      
        1 gaccttgata gagataccga gcttcttgcc tgccttttcg agtgcctctg ccgcaatata
       61 ggtatgtgct atacctgtag gacaggcggt aacagcaagc acctttatcc tgtcggtgtt
      121 ttcaggtgct tcctttgctg tcggctcatc gggatacttt gccttttcgg ctgtgtctat
      181 taccgataag aactcgtctg tatcctttgc cgaaagaagc tttgctctga aatcctcgtc
      241 cataagcagt gtcataagac gtgaaagcac ctcaagatga acgtccgcct cagtatcggg
      301 tgcggctatc ataaacagta cgtctgacgg ctcatcgtca agcgcttcgt aatcaacgcc
      361 gcccggaacg gtcatagccg caagcgaggg ggcttttacc gcactgctct ttgcgtgggg
      421 gatcgctatg ccctctccta ccgctgtcga gcctttttcc tcacgggcta aaatgccctg
      481 cttgtatatc tctctgtcgg atatgttgcc gctcttcatc tgaagatcga ccagcatatc
      541 aatagcctct gtcttgcttt tcggtgcacc gtttaaaaga atgctgtcct tatttaataa
      601 gtcaactatt ctcatttttt tcctcctttt tcggtttaag ctaattgctc cataagctcg
      661 tctattgttt tcttttctgc aagtccatcg gaaaatgccg ttgcaccgcc ggacgctgta
      721 ccaagtttaa gcgcatattc atagtcgccg ttttcgcagc ctgcgataaa gcctgccacc
      781 attgaatcgc ctgcacctac ggaatttctt accttgccct tgcaaactcc gcagatgtga
      841 gttttgccgt gttcgtcaag aagtatagcc ccgtcgcctg ccatcgacac aagaacattt
      901 accgctccca tctctctgag ctttcctgcg tactttatta tatcctcatt ggttttaagc
      961 tctacaccga acatttcgcc aagctcaaag ttgtttggct taataaggaa cggcttgtac
     1021 ttgaggacgt taagaagaag atcctttgtt gcatcgacta caaaccttat tcttctgtcg
     1081 gcaagctgtg ccattattct ctcataaatg tccgatggaa gcgatgaggg gatactgcct
     1141 gcaagcacca aagtgtcacc cgactttagc ccattcagct ttatgaacag ctcattgatc
     1201 gcttcctgtt tgatttcggg tccctgtccg ttgagctctg tttcttcacc cgatttgatc
     1261 ttgacattta ttcttgaaaa tcctttatca aggtgaacaa agtccgcgtc tatgcccttt
     1321 tcggctatac ccttttcgat cgcctcgccg gtaaagcccg ctacaaagcc gagcgctctt
     1381 gacttcacac caagctcaga taacaccagc gaaacgttta ttccctttcc gccgaagtat
     1441 atctcctctc tgtccgagcg gttcaccgct cccgctgtca gctcctttgt gtgtacgata
     1501 taatcaacag cggggttgaa agttaccgta tataccattt atcctacctc cgttatatct
     1561 gccgcattgt tatactttct gttcggtatt ctgtctgtga ttatgacagc cttgccaagt
     1621 cccgcaaatg ttgctgagct tacctcatca aacttagaat ggtctgcaag aacgtaaacc
     1681 tcgcgactgc tctctatggc ggcagattta actctcgcct cattgacatc gggtgttgta
     1741 aaccctgctg tcagcgatat gccgttagta cccataaagc actttgtgaa gttgtagttc
     1801 ttaagagtga gtacgcattc cgccccgact atcgcctctg tgcttgcttt tatttctccg
     1861 ccggtgataa acaccttata gcccttacga gccagcttct tagcgtgaat aaagccgttc
     1921 gtaacaaatg tcacgtcctt cacattcaga tagtcgatca ttttctcggt cgttgtacct
     1981 gcatcaagga acacgaagtc accattttca ataagctctg cggcatattt tgcaattgtc
     2041 attttttctt cggtacagat agaagctttt tcttccacat tcttttcaac cgatgtaaaa
     2101 ctttcgttcc ttgacatcgc tccgcctcgt accttgatta ttttaccctc atttgcaaga
     2161 ataatcaggt cacgctttac cgttgatgcc gaaacgtcca gcatttcggc aagttctgcg
     2221 atagttacgc ttgcacgctc gtttaccgct ttgattatca ttgaatgacg ttcttctgtc
     2281 agcatattaa gacctcttat aaatatcatg atattacttg tttgagctgt tttaagctct
     2341 tatggactgt tgctctctgt ccttgagcta ataataacat acctaaagtt caaattcaag
     2401 cattttagct cagaaacatt caattctgcg tgatttttgt tgtgtttaac aacaaatgtg
     2461 agatgaattt gtgcacaatg aacaaattcg agcaattgcc aatcgctacg gttcgcatcc
     2521 gctcaatatt gacaaattca gctcaaatat ttatttttag ctttgcacca aatacacgcc
     2581 gaatttcaaa aatcgtttca tttttgagat aaaagtaccg ttttgagtac aaactttgta
     2641 aaaatgcgcc ttttttgtga agnnnnnnnn nngtatgaat atcggcgcgt aatttgcaaa
     2701 aatcgttgat ttttgtgaat aagtgtgcta caatcgtact ataataacaa aaaggggtgg
     2761 taatatggat aaaaagccgc ttaaaaagct ttttcacatt gataaagaag cttacaaaac
     2821 ggaatatgaa aagaaattca acgatgaaga tactatacat cttgatatag agatttcggg
     2881 caatcctgct ttcatatgtc agacaaaaga agtgttcggc agtatcatct ccatcgagcg
     2941 caccaatact gcaattgcaa aaatgtgctt cgagcttccg gaaaaagcgc ttatacaata
     3001 ccgcaaccgt tgccttacag atgagatcgt actcacaaac aacatagagg gcgtacacag
     3061 cacacgaaaa gaaattaacg agatactcac agacctttca aagaaagaca gaaaaaaacg
     3121 ttttgtcgga cttgtcaaaa aatatgcggc gctttcacgc aacgaggaaa tacctctgca
     3181 aaccagccgg gatataagaa gaatatatga cgatattttt tacgatgaga taaatgcaac
     3241 cgacccgggt aatcttcctg acggcgaaat attcagaaaa ggcgatgtaa gcgtatattc
     3301 gcctacacaa aaggttatcc atacaggtat tcacccggaa gcggaaataa tatcgataat
     3361 ggataaatca ttggattttc ttgaaaactc cgtctgtgat atcatgataa gaatagctgt
     3421 gtttcactat ctgttcggtt atattcaccc gttctatgac ggcaacggca gaacagaccg
     3481 ttttataagc agttaccttt tatcaaaaca tctcaatgac cttatcggtt atcacatttc
     3541 ttatacgata aaagaaaata tccataagta ctatgacgct tttgatatct gcaatcaccc
     3601 tctgaacaag ggcgacctga caccttttgc agagatgttt cttagccttg tcgacatttc
     3661 tatgaagcaa ttatatgacg agatcaaaag ccgacttgat aaactcaatt tctacagagg
     3721 tgtgtgtccg atcctgccca acgcagatca taaagatatt ttaacgctgt attatgtgct
     3781 gatacaggcg gctttgtttt ccgaggatgg tatatcccaa aaagcgctcg aagacatcct
     3841 gaccgcttcc tactcctctg tgcgcaacaa gctaagctgt ataccgggcg aattgctcat
     3901 caaaaacaca agggacaggc acgcgtatta tatgcttgac cttgataagg ttgatatgat
     3961 gttttcaaaa taagaacagc ggcagactgt ttaggcggtc tgccgctgtt cttatttacg
     4021 gttgttgaaa tcttcctcta tttcctcgct ccagttatcg tttatcctgc ccttactgcg
     4081 cattgcggtg agtggagcgg aaattgcatc atagatcacc tttgtacctt tcttatctgc
     4141 gtgataatta acggctctgg agtgttcgat gccaaaagat tcggctgtga ctattgcgtc
     4201 tatatttgct ccgataaaga gaaattccca gccgtatttt gatctctgac gctctatcat
     4261 acgttttata tcactcttgc tgtaatgacg gcttgcgttt tccagaccgt cggttgttat
     4321 cacgaatatg gtgtgagcgg gtatatcctc cggacgtgcg tacttgtgga tatttcctat
     4381 atggtttatt gctccgccta ttgcgtcaag taatgcggtg catccgcctg cggagtaatc
     4441 gtcagccgtc atcggcttaa cggcgttgag agccgctctg tcgtgaagta cggttgtcga
     4501 tgtgtcgaac agaacggtgg aaacgtatgc ttcgccggta tgctttttct gcttttctat
     4561 cattgcgttg aatccgccga tcgtgtcgga ttcaagacct gccattgaac cgctcctgtc
     4621 gagtatgaat accatctcgg tcgactgatt tttcttgttt gctcttgctg ttttaacatt
     4681 tatctttttc atgttttttc ctccgcttat taatacagat tcggggtttc ttatcttctg
     4741 tctgtatttt agcatataag ggaataaaaa tggtcgcatg aaaagcgaca aatgaaaaat
     4801 cctgcgattt gtatgtggat cgcaggatct gatttatgcg ccgagcaacg tctgatcgta
     4861 agagaacagc gtttcgttta ttgtgattat gtcgtatatg ccgtttttga taaaatactc
     4921 tattataatg tcaaacctgt tgctgtgcga cagggcatag cctgctttca tcagcagctc
     4981 tgcggtttcg gggagcgaca gaccgagcgc tatcgcaaaa gcaacggctg tgggctttga
     5041 cggcttataa tcaatattgc tgcggatctt tgaaaacagc ttgcggtcaa tgttagcctt
     5101 tttatagcac tccgaatcct tcataccgct ttcatctatc ttgcgcagga gcatctgcga
     5161 gaagctctca tctatactgc tcagtgcatc ctcaagcgta ctgccttgtt ccgtttctgc
     5221 ttcggcttcg agaacacaca tatcgtcaaa cgcttttttg cgtgcagttg cagccgtagg
     5281 aaagcaatgt gtggttgcta tgtcctgcag ccttccactg aataatctgc ggcgtttttc
     5341 agccggttca tcggtatggc tctgagcgta agtatcatca atgtagcttt ctatatccga
     5401 aaacagcttg tttgccctta gaaagctgtc actgtcaaaa atgacaatat agaccgtcat
     5461 atcgtcagcc tgttcgtgag tgccgagaaa atcgagaatc gtatctgacg caacacggaa
     5521 agcctgttct ttcggatagc cgtaagttcc tgctgaaatc atcggaaacg cgaccgtttc
     5581 acaaccattt atcaaagcga gcgacaagga atttctgtaa cagctttcaa gcagttgctt
     5641 ttcgcctgat tcgccgcccc gccatatagg acctaccgtg tgaattatat atctgcaagg
     5701 cattttacag ccgcttgtga ttttggcatc acccgtcctg caaccgccaa gtgtgagaca
     5761 ctccttgtaa agctcctcac ctgcggcacg gtgtattgcg ccgtccactc cgccgccccc
     5821 gagcaatgat tcattggcgg cattgactac cgcatcacaa ggaaactttg taatatctgc
     5881 acggactatc tttaacggca taattttctc ctttatttaa gaacggcttc gatcgcgctt
     5941 atctcctcat cagtaaaatc aagcttattt atacactcaa cgttttcaac tatctgtgcc
     6001 gggcggcttg ctcctataag tacggttgct accgccttat tatgaagcac ccacgaaagc
     6061 gccatctgcg agagcttctg tcctctgctc agcgcaattt cattcagagc attaagacgc
     6121 ttcagcatta tatcatcaag ctgtccctca agccacggcg cgccgccttt agcaagacgt
     6181 gaatcttcgg gaacgccgtg gagatactta tctgtaagaa agccctgata aagcggcgag
     6241 aacaccgcaa gactcagatt attgtcaagg cagtacttat ctagtccgtc acgctccacc
     6301 cagcggttca gcattgaata tgacggctgg tttactacaa agggtgtcct aagctccctg
     6361 aaaatcttct gtatttcctc tgtctgctgt ttattgtagt tcgatattcc tacatacaac
     6421 gctttaccct gtcttaccgc attgtcaagt gcgagtgcgg tttcttcaag aggtgtataa
     6481 ggatcaaaaa tgtgatgata gaatatatcg acatagtcta tcttcatacg tttaagactt
     6541 tggtcgagcg aagcagtaag atactttctt gaaccgttct tatcgccgta aggaccgggc
     6601 cacatttcat aaccggcttt tgttgaaata cacatttcat cacggtattc cctcataccg
     6661 cgatcaagta tcagcccaaa gttctcttct gcgcttccgt tgtaagggtg tccgtagtta
     6721 ttagcgagat caaaatgtgt tataccatgg tcgaatgcgg tatgtaccat tttttccgcg
     6781 ttggcataat ccgaaccgaa gccgaaattc tgccataaac ccagtgaaac tgccgaaact
     6841 ttaagtccgc tgtttcccag tcggttatat accatttttt cataacgnnn nnnnnnnccc
     6901 tgtaatatcg ggaatgcgat ccaaccgagg aaaggaatta taagaacgaa tacagaaagg
     6961 atagaaacaa cggttatctt aagagcctgt cttacatgga acattgtgta ctttgatgag
     7021 tgagttgcga ggagggcaac aatgatgccg ataaaatcca taaggtaaca aagcatagca
     7081 aaaaccttgt tgtcggatat atccttagga tcaaattcag ccgtatgatc atacggatca
     7141 tagcccggag cagccgcata cgctccctga ggaggaattg caccctgctg aggcggtata
     7201 gcattttgct ggggaggtac tgcattctgc tgagggggca ctgcaccctg aggttgcgca
     7261 ttgccgaact gtacaccaca agtcgtgcag aataaagcgc tgtcatccaa ctgtgttttg
     7321 caattaggac atgttttcat attcattctc cttgattacc gtgtcttaca gttattgttg
     7381 actttcctgt agacgaatat tgtaatttct tgctattatt atagcatttt tgttccggta
     7441 tgtcaatgaa atggtgtaaa aaatgaaaaa attcaactat caaaaattaa aatcaattag
     7501 atcagctcaa atcttcgata ttctcaattt caacaactaa ccacaatttt atcattatta
     7561 tatgcgaatt tacttttcac tatacgacct taccgttaaa acctaagcat ccatagcaac
     7621 aaaaaagcac tgcctgagca gtgcttgaat ggctccccct gttggacttg aaccaacggc
     7681 atcgtgatta acagtcacgc gctc
//
LOCUS       DAWAOG010000020         7610 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1403_length_7610_cov_2.414692, whole genome shotgun sequence.
ACCESSION   DAWAOG010000020 DAWAOG010000000
VERSION     DAWAOG010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7610)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1403_length_7610_cov_2.414692"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    484..493
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1029..1038
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6272..6281
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7297..7306
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1712 a         1894 c         1542 g         2422 t
ORIGIN      
        1 ccacgagctt atttttttcg acttcgagat ttatatcctt gagcgcatca acggtagtat
       61 tatcattgat cttgaagctc ctggatatat tctcagcctt taaaatcgtt tccatagtca
      121 gttccgatcc tcaaaaaagc acgcctgcgg ttaaacagcg cgtaacttat ttcatctgct
      181 ttttgtacca aatgcacaat aactacatac gattttgccg ttaaacacgg tgtatttaga
      241 aataatgtac aaagtggcat cagggtctat aacatagtaa ttatatcaaa ttatcaccgt
      301 actgtcaagg ttcataaaag aactttcaca tagtgtaact gattttgatt ttatctacaa
      361 atcgcatttg ctcttagcaa atagttacaa acttagctcg aaaattttgg taattatgac
      421 gaacgcggtt ttcattgaca caatatcttg atttttagtt ccccgacttt gctatttttt
      481 atgnnnnnnn nnntttatgt aaacgttttc cacattacga aaacttataa cttaaattag
      541 ctaattcgtt aatcgtctta acgaaacttt gttgattgtg cacaaagaat ttcgtcactt
      601 tttttaacta atcgcacaga taattactta atttttgatg taacttatga aaacgtttct
      661 atctgctcac tgagctccat ccactgagcc gtaagttcct cttctttttc tttcaaaaca
      721 gctatttctt cggaaagttt gcttgctttt tcatagtctg tagctatctc cggctttgac
      781 atctgctgtg caagctctgc tgtttttgtt tcgacatctt ctatcgcctg ttcgcagcgc
      841 ttcagtgcgc cattcagttt cctgagtgcg ctctcacgct ccttacgctc tttataatcg
      901 ttcttctttg cctcggtaac agctttcgcc tgaactgcca ggttagtctg ttccgcctgt
      961 tcctttgccg ccttcagttc aatatacctg tcatagtttc cgtcaatgtt gaccgtgccg
     1021 ttcttatcnn nnnnnnnngt tcttatcaag ccacactatc ctgtccgcaa gcttgtttat
     1081 gagataacga tcgtgtgaaa cgataagaag cgtaccgccg taatttgaaa gcgcattttc
     1141 aagcgcctct ctcgaatgta tatccaaatg gttagtcggc tcatcaagaa gcaacatatt
     1201 cgccttgccg agcataagct tcaggagcga cactctcgcc ttttctcctc cgctgagctt
     1261 ccccacattc ttgaatacat cttcaccctt gaacaaaaac tgtgccagtg ccgatcgtac
     1321 ttccgtctgt gtcattctcg gatacttatc ccacacctca tcgataaccg tctttgacgg
     1381 atcaaggtca gtctgcgcct gatcgtaata gccgcactgt atattgtttc cgtatttata
     1441 ctccccgcta tcaggctgat actgtcccat aaggatctta agcagtgtcg tctttccaca
     1501 gccgttctcg ccgagtataa acgtggtcgt attccttttt atatcaagct ctgcgttttg
     1561 gaaaaccgtc acgccgtcaa acgactttga cagagctttt gcaatcagca cgtcatttgc
     1621 tccgccctct ttcgctctga atgagaactt tatagcttca ggcagatctt cgggtctttc
     1681 aagcgtttct ttgagcctgt cagcctgttt ctgctttgat gccgctgtaa tgaaattatg
     1741 ttcctgtccc cagcgcttct gctgttcgac tataccctcg atacgattga tctcttttac
     1801 agtcctgtca taaactcttt gctgtgcttc tctcgcctcg gctttcagtt caaggtagcg
     1861 cgtatagtta cccttatatt catggagcgt cttattctcc atttcaaagg tggtattcgt
     1921 gaccttatca aggaaatagc ggtcatgcga tataacgata taagcaccct tatattccgt
     1981 gaggaacttt tccagccatt cacaagcggt gatatcaagg tggtttgtcg gctcatcaag
     2041 caaaagtaca ttgctagcgg aaagcaaaag ctttgcaagc tgcgccttgc ttttctgccc
     2101 tccgctcata acgcttatcg gcttattgaa atcatcttcc gaaaagccaa gaccgataag
     2161 tgcagaagct actcttgact tgtaagtaag tcctccgtca cgttcaaatc tttcctgtaa
     2221 gtgcatctgt tttgacagcg tctgttcgct gtgatcgccc gtatcaatgg caaccgctat
     2281 atccgcaagt tcttcttcca tgtctgtaag cggacggaat atatcaagca cctcactata
     2341 cagcgtcacg ctgtcgtcac ggataacata ctgctccatt gaaccgagct ttatacccgc
     2401 cgccttgttt atactcccgt tttcgggttc aagcttgccg tttatgacgt gcataagtgt
     2461 agtctttcca cagccgttga ctccgacaag acccgccctg ccgttttctt caaggcgaaa
     2521 attcacattg tgaaaaagcg tttctccgcc gaatgatacc gagatatcgt taccggacaa
     2581 tactatcatt cgagtacttt tcctttccga aaaatagcgg tcagagtttt tgctctgacc
     2641 ggtaattcca tatttacgtt tttgcttact tctttaagtt ttcgggcttg tacctgttca
     2701 gcgcgtcatt attaagatca agcgcatagt cctttgctgt ttcgttcagc atagcctcat
     2761 aatcgtcgtc tttcataacg tgaagcacgt tgctgtgatt ctccgtgagc cagtcctcac
     2821 gttccttgat atcgcgacgt acaaccgcat agtaaacggt atcgtcctca taaacgccgc
     2881 ccttgcctgt ttcaagctca aatgcccact ttacaaattc ttcgctgggt gaagtatccg
     2941 tcttgctgag caggaactcg tgatcgtgct cttcttcgtt gctgtcggat gctccgctgc
     3001 ttgttgtatc gtcagatgct gtttcacttg cagaagaaac agccgacgag ctttcggagg
     3061 aagcctttga agatgcgccc gagttgcttt cagcttcggc atctgccttt tctttcgcta
     3121 cctgatcctc gtattcgtga taaatggttt cgtaatcctc accggcaagc agtctgtcca
     3181 catagccctg agcctgttcc ttctttgcct tcttgccctc gtcggtcgtt atcttgtcgc
     3241 cgttgccctc tttaagagaa acctgtatta tctggaatct gccgtaattg tcatagaaat
     3301 aagtagctat atccttttcg tctgttgcgg atatgccgtt tttctcatag taagcgtcaa
     3361 ataccttttc gctcatcttg tttacaagct gaacagcctc gtatgacttc ttgcttattc
     3421 cgcctgcctc atagttctga ccgtaggttt ttcctgtgct gtagccgtaa tacgagatct
     3481 ccgtatccca catcttcttt acagaggtct ttacattatc cttttcctca tcggtaagag
     3541 taagcttcag ctcgtcaaac ttcttctgga tagcaacata ctgctttacc tgctctaatg
     3601 tacgatcctg aacatactgc gagtatgtct tgtcctgcac gttatagttg aaatagctga
     3661 aatcctctac ctttgaagaa tcagtaccca tagccttcag ctgatcgtcg acttcctcct
     3721 gagcctcgct tatagccgac tgttcgtaca gcagatatat gcctgcattg atggtctgat
     3781 catcgataga acctatagtt gatacatcag cacagcctgc aacgcttaat gtgattgtca
     3841 cggcgcatat tgccgctacc agctttttta aatgtttcat tgcttttctc cttactgttt
     3901 tctataaggg gctgtgcccc ggttattctg taatatttgc cgttatctta atgtaacgcc
     3961 gatagcttcc agcgccttaa gcacctttgc aacgaccgca tctgcctcat cgtctgtcag
     4021 cgtagcatct ttctttctga gaatgagctt gtaagaaagg ctcttcatac ctgcgggtac
     4081 ctgttcgccc ttatacatat cgaacaggcg taccgcttca agatgttttg cggtcttttc
     4141 gatcaccgca actatatcgc cgctcggcac gtcctcgttg cacacaaggc tcagatccct
     4201 gtatgttgag gggaacttgg gcagggcagt gtacttcaca tcgccgtctg cgttattgaa
     4261 cattacttcc atactgagtg tagcgcagta aacctcagta tttacgccga agtttgccgc
     4321 agcctcgggg tgaagctgac ctacatatcc gataagctcg ccgtttacag tgacctttgc
     4381 gcatcttccg gggtggaatg tggggttgtt cttttcggct gtgtatacaa cattcttcag
     4441 accgagcttt gccattactt cctctgcaac gcccttggct gtgaagaaat cttcaccgtt
     4501 gccgtagaga gcatagcaca gaacatctct ttcaacgggg agctgatttt ctcctgtcgg
     4561 gaaatattct cttgctatct cgaaaaatct gccggcaagg tttctgttgc tccagtttct
     4621 tcttacgacc tccatcatag aagtgagagc gcttgtacgc attacgcttg tatcctcacc
     4681 gagcggtcgt cttaaaacaa cactctttct tgactgctcg tccatacgga gcatttcgta
     4741 gtccttgggt gaaataaagc tgtatgtcat tacctcgtaa aatccgagcg caagcgcaag
     4801 cgaaattacc ttatcctcga atatctgctc aggtgttctc ttgccctgtg atgagagctt
     4861 gggtatagtt gagggaatcc tgttgtagcc gtaaatacgg gcaacttcct ctgcaacgtc
     4921 gcacgcacgg tgcatatcta tacgggtagg aggaactatt atctcgccgt tctcgtagcc
     4981 gaagccgagc ttctttaaaa tttctatctg ctcgttttcg ctgatatcag agcctaaatg
     5041 ctcatttacc cacttgtagt cgtgcttcaa tctttcggga acatactcgc tgtggttctc
     5101 gtcaatgata gtattcacta cctcgccgca acccagcatc tcgataagct gtaatgctct
     5161 cttgagcgcc ttttctgcat ttacggggtt gagtcccttt tcaaagcgtg agcttgcctc
     5221 tgtacgttct cctatctttc ttgcggtacg tcttaccgat acgccgtcaa agcaggcagc
     5281 ctcaaataca actgtagtag tatcgtccat gataccgctg tgctcgccgc ccataacgcc
     5341 tgctatggcg ataggcttct tgccgtctgc gattatcagc atttcgggag tcagctttac
     5401 aggctctgcc atttcatcga gcagctttaa tgtttctccc tcttttgcgt ttcttacaac
     5461 gattttgtta tcctcaacat atcttaagtc gaatgcgtgc atcgggtgac cgtattcaag
     5521 cattacaaag tttgttatat ctaccaaatt gttgataggt cttactccgc aagctctcag
     5581 tcttcttgcc atccacttgg gtgacggtcc tatctttacg ttctttacaa gcgccgccat
     5641 atagcgtgag caaagctttg tattctcaac gcttacgctg atttcctttg tgatatcacc
     5701 gtcaacgccc ttgaatgtcg gctcggtgta attcatcggg atatcgaatg ttgcgcttgc
     5761 ctcctgtgca agtccgagta cagaaaggca gtcgggacgg ttgtttgtga tctcgaactc
     5821 tacgatagta tcatctatct gcaaagcctc gcagatatcc atacccatct taaaagaatc
     5881 aacatcgggg tcgttgttca gtatgagtat tccgtcatct gcgacagggt agtcgaaatc
     5941 gtcctttgta agaccgagct ctgcgtatga gcacatcata ccgttgcttg caacgcctct
     6001 gagcttaccc ttagttatct ttgttccgtc ggctattgtg ctgttgtgca tacaagcggg
     6061 aacgatcgca ccctcaaaca ggttctgagc cgcagtaact atctgcacag gctcatctct
     6121 gcccgcgtca agctggcata tccagagctt ttcgctgtcg gggtgacgtt caagcttgag
     6181 cacctttgca actactacat tatttatagg gtcggacatc ttatgatagg tttcgacctt
     6241 tgaaccgctc atagtcatat ccgctacaaa tnnnnnnnnn ngccctcgcc cttacaaagc
     6301 gggcagccct tgccgccgca cttgaagcac tgtaagtcga cctcggctga cggctcggtg
     6361 tacgggaagt ggtgaggacg gaaacggagc tttacatcgt cgccgtaaag acgctttgca
     6421 aatgtttcga gcgtaccctt aaggtcgccg aatgttatat tcttgtcaat aacaagaccc
     6481 tcacactggt ggaatatagg agagtgcgtg ccgtcaacag catcgggacg ataaacgcgt
     6541 ccggggctta tgatagctat agggggcttg tttttcagca tcgttcttat ctgaacggaa
     6601 gaagtctgtg tacgcaggag tatatcatct gtgatgtaga atgtatcctg ttcatctctt
     6661 gcggggtgac cgtcctcggt taaaagcata tcaaatacat acttcgtttc ctcgacctcg
     6721 ggaccgtcaa cgaccgtata acccattccc ctgaatattt ccttaagctc atcaagaact
     6781 atattaagag ggtggcgaac gcccatagcc tgctgtttgc cgggcatagt aacatcaagc
     6841 gtttctttct ttaatctctg ctccataagc gcagatttaa gctgcgactg cttctgtgca
     6901 ataaaacttt caagcgactg ccttacatcg tttgcaagct gacccataac aggtctttct
     6961 tctgccgaca gctttcccat gctcttaagg atagctgtaa gctcaccctt cttgcccaga
     7021 tattttactc tcaggtcatc gagctgtttg acatcaacgc agtcaccgat agttttcagc
     7081 gcctgctcct ttatattctg taactgttct ttcatacctt ttattctttc cttattttta
     7141 atatccttaa cttaaagtcc gccttcaggc aaagacgatg gaaccgaata gttccactga
     7201 taacacgacc tttcacgcat ttttcagcgt agatgaaaat aatttatggt tatatagata
     7261 ttacttttta tggaaattta aatcaaagcc tataccnnnn nnnnnngatt acgacagggt
     7321 aaaataaaca cccgccgtga aagtggtgca ttgtgtagaa aagtctgtta tatggtgtac
     7381 ttcttgttat atcgttttgt atttactgtg attgttggct gccgatagtg cctttcgtgg
     7441 ggtagcactt ctcgggttcg ctattccgtg cgatgtgttt ctgttttttt gcttggtgga
     7501 aatggttggt gtttttgctg tgatagcagg ctaccgatag tgctttttgt cgggtagcac
     7561 ttctcgggtt cgctattctg tgcgataacg ttgctactat gtgcgaggga
//
LOCUS       DAWAOG010000021         7578 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1413_length_7578_cov_2.273827, whole genome shotgun sequence.
ACCESSION   DAWAOG010000021 DAWAOG010000000
VERSION     DAWAOG010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1413_length_7578_cov_2.273827"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2393..2402
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5314..5323
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6581..6590
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1995 a         1735 c         1665 g         2153 t
ORIGIN      
        1 gttataagtg acgcgccgat aaagaaaagt ataaaccacg gaaatatctt ctttatcgaa
       61 aaatccgcct tactgcctcc gcttttcttt gcttttgccg tgcggtacag tgcaaggata
      121 agggtaatag gtatgatcgc aagcgttctt gtgagcttta ccgttactgc ggccgatagt
      181 attccgttat agccgtaaat gccctcggca gtcgatgccg ccgcagttac cgatgaggta
      241 tcgtttaccg ctgtgcccgc aaagacagca aagccctctg tgccaagtcc tatagcgtgt
      301 ccgagcgtcg ggaatataag tgcggcaagc acattgaaca ggaatattac cgatatggag
      361 cgcgccacat cttcatcgtc cgcttcgata acgggcgcgg tagccgcaat agccgaaccg
      421 ccgcagatag atgagcctac tccgataagg caggcggttt tcggatcgac cttcattatc
      481 ttcatcatta taaatgcaac tatcagcgat attgaaattg taccgattat gacaggcaga
      541 ctttttgcac ctacctttgc tatagtgctc aggttcagcg aaaatccgag tataaccacc
      601 gcccattgca atatcttttt tgaggtgaat ttgatcccgc ccgacagtgc cgaaagaccg
      661 gcgagcttag gaaatataag tgtaagtatc ataccgagaa gtatcgctat cacaggcgca
      721 ccgataaggt cgaacgaaaa cccgccgatg tttattccgc ccgcaagcgt tgcaacagcg
      781 gcaataaccg cacacaccgc aatacccggt attattgatt taagcttctt cattttctct
      841 ccgtattgta aaccgacata aaatgagcca tcgtcaccga tggctcttaa tatttgctct
      901 tttaacgatt attcagcgtc gttttcagct gcttcttcct cttcctcagg catttcttcc
      961 ttgagtgcgc ggatcgaaag gcttactctg ttctgctctt cgttgatctc gattatcttt
     1021 actttaacct cgtcaccgat agaaagaacc tgagctacat tgttgatacg ctctgttgag
     1081 atctggctga tgtggataag accgtcaacg ccggggatga tctgtgcgaa tgcaccgaaa
     1141 ggagtgatgc ttacgatagt tacgtcaaca acatcgcctt cgtgatattc agcaacaaac
     1201 ttgttccagg ggttgtcctc ttccttcttg cagccgagtg atactctctt cttttcgggg
     1261 tcaaagctct ttacatatac atcgagcttg tcgccgatct taacgatctc tctggggtgc
     1321 ttgattctgt tccatgtgag ctcgcttgag tggaccatac cgtcaactcc gccgaggtct
     1381 acgaatacgc cgtagctttc gatagactta acctcacccg tgaacttctg acctacctca
     1441 actgtttccc agaacttagc cttagcagcg tcattagctt ccttcttagc ctgacggata
     1501 gaacctacaa ctctgcctct tgcttctgtt acttcgataa ccttgaaagg aactgtcttc
     1561 ttgagaagct cttcaagctt gccgtccttg ggaacacccg tctgtgaagc ggggataaat
     1621 actcttgtac cgttggagat aatgataacg ccgcccttaa ctaccgatga tacctcacct
     1681 tcgagcgttt catttgcttc catagcagcg gcgagctttt ctacgccgag cacactgtca
     1741 accttcttct tggaaagtgt gactgtacct tctgcatcgt ttacggctgt aacgatagct
     1801 tcgatctcat cgccaacctt aacaacatct gagggatctg cattgggatc ctgcgagagc
     1861 tcgcttgcag gaatgtagcc cgagtgcttt gtgcctaagt ctacaacagc ttcgttcttg
     1921 tttacagcta ctactaaagc cttgaccctg ttccctatat atactttttt gaaggtcttg
     1981 tcaacctccg ccatgaagtc gaatccttct tcattgttga gaatttcgct cataattcta
     2041 tgaacctcct ttattatata ggcaggtgtc gaagctcctg ccgttatacc gaccgtaccg
     2101 ccctgcaaag ggagcagttc cgatactgcg gctttatcaa tatcactgcc gtcctccgtg
     2161 aatattacgg ggcagtgttt ttgacttatc tgtgcaagct ttgctgtgtt gctggagttt
     2221 ttccctccga ctgtcaccat aagcaccgag cgttccgaaa gctcttctgt acggtgctga
     2281 cgttcagccg ttgcactaca tattgtatca aaaatcgcga tatttgtata gtgttttttt
     2341 gcaacttttt tgcaagattg ccattttgac gaattaaacg tcgtctgagc tannnnnnnn
     2401 nnacctcagg gtggtcgggg tcgcccgcca caataacatg accttccgct ttggaaacta
     2461 tatcatgtat ctttgcgaca aacggacagg tagcgtcaat gcagtttatg cccttatcgt
     2521 tgatatgatc tataactgac tgcgccactc cgtgggagcg gataataagc gttttaccgt
     2581 caggcagatc atcgggtgaa tttatcggat atacaccgag cctttcaagc cttgctatct
     2641 cgcttccgtt gtggataagc tctccgagcg tacagcagcc gccgttttca tcggcagtct
     2701 tttctgctat ctgtacggct cttttgacac cgaagcagaa gcccgcgccc tttgcgatct
     2761 caatattgta acggctcact tattatcctc atcggactgt ttttcgactt taggaacgta
     2821 ctgcttgggc agcggatctc cgtattcgct cataccgtcc ataagcatct ttatattatc
     2881 cattataagg tttgaaaccc gtctgagttc ggttctgtcg tcactgttca gtgcgatatg
     2941 ctcggcgggt atcatcttgc ctaccgagat ccatactttt ctgcggaagc cctttttccc
     3001 gtactttatg cacatcggaa ggacaggagc gtttgttttg cctgcgatga ccgcaattcc
     3061 tgattttgct ctgccgatat tgccgtcctt tgaacgtgtg ccttcgggga atattacgaa
     3121 agccctgttc ttctcaaggt cggctatagc gtggtcaatg gcactgccgt cgcctgcacc
     3181 tcttacaact ggaaatgctc cgcactttgt aataagccac gcgaagaatt tatttttctc
     3241 gaacagttcg atcttcgcca taaacgaaaa atgtgccttg ttcacagccg caataagcgg
     3301 aggatcaccg taatcctgat ggttgcaggc gattatatag ccgcccttta tgccgttctt
     3361 atacggtctg ttgtcaccgc cgattacctt gatcctgtat tttatgtgca ttgaaagaca
     3421 tactatcttt ctgaaaaact catacattat ctgatgtctg ccttttcttt gattattctg
     3481 acgagcaggt cgcacgagcc ttcaaaatca agctcggtcg tatccgcaag cactgcgtca
     3541 tcggcttgct tcagcggtgc tatttctctt gtcatatcgt tatgatcacg ttgctttaaa
     3601 tctgtaagca cattttcaaa cgacaccgcc gtgccctttg caataagctc gtcataacgg
     3661 cgctttgctc gtacctctgc gtctgcagtg agatatatct tcaccgttgc attaggaaga
     3721 actaccgttc ctatatctct gccgtccatt ataacgtcac ggctttctgc catatcacgc
     3781 tggaactgaa gaagaaacgc tcttacagcc gggacagcag aaaccttgga agccgccatg
     3841 cttatctcgg gtaagcgtat ctcttccgaa acgtcatatc cgttgagata tatgtgctgt
     3901 gtgccgtcct ttatcttaac atcaagagaa atatgcgaaa gtgccgcgct tactctcaca
     3961 gggtcgtcca tatcaacgcc gttacggtcg cagaaaagac cgatacagcg atacatagca
     4021 ccggtgtcaa cgtatataaa gcctaaacgc tttgcgcatt ctttggcaat ggagcttttg
     4081 cccgctccta cagggccgtc tatagctact gtaatactca tatttgttat tctccaattt
     4141 acatatacat taagtatacc ataaagacag ctcgaatgca acagggaaaa tgtgaaaatt
     4201 aagcgaaata aacagctttt aagcctgtag agcgaagaaa attccccgaa aaaccaaaat
     4261 gcttttcggg gaaatatgtc agtttgtttt tgaaagctgt tttttctgcg ctcttttaag
     4321 atacgcagga gaaagcagac gcagttgttt ttttacggtg tttctcacct cacggcagtc
     4381 ggagatatag ctgccaccgt ttcctttgcc tgccatatac tgtcttgcta tcattccgta
     4441 cagcgttata agcaccttta aggtcataac ggaaaccctt gtgcgacagc tgtaatagat
     4501 gccgttttcc gcaagatatt ccgacaagat gagatatgcc tcaagatagc tgtccaccat
     4561 ttttcttccc gcaagacccg ttatgctgtc gggacggtgg gcatagctgt aaagcacctt
     4621 tcgtgtgaag gcgacatttt ccgcacaggc aaacaggcgg ggcgttgtaa taacgtcctc
     4681 aaagcacttt ccgacagggt aggatatatt gttgtcggtg aagagcgaac gtctgtacaa
     4741 cttgttccat gcgtaatttc ttaccgaaac atcggaaatc agcttttcaa acgccttttt
     4801 tccgccgatt acaccgttac ggtggcaaag aagcacattt atactgcggt cggatttttc
     4861 aaagtaattg cagtatctgc actgtactat gtctgcgttg ttttttgcgg cgagcgtgta
     4921 catttcggaa atgtaaaagg gcgatacaaa gtcgtcgctg tcaacaaagg cgatatattc
     4981 gcctcttgcg tttgcaagac ctgtgtttct tgcggctgaa agacctgcat tttgctgagt
     5041 gattattttt atgaaatcat actcgcttgc aaacctgtcc gctatcgtca tagaattatc
     5101 ttttgttccg tcattcacaa tgattatttc gtatttgtat ccgaacttct gacggacaag
     5161 cgaatcaaga caacgttcaa tgtatttttc aacgttgtat acgggaacga caacactcac
     5221 ggcgaccgca cgctctcgca tattatcagt gttcatataa acctctcctg aatagaaaat
     5281 aattgcagat agaaaactcg gactatagca tatnnnnnnn nnnatagtat atcggggaaa
     5341 taaacaaatt ttactatgca aattatacaa ctaaaaaaaa tagcagtcaa tcggaaaaac
     5401 gtgtaaaagt ttgtaaaata aaaaaatacg gcagtttaac gaataatcat atgcttaacg
     5461 gctgataatt aaacaaaaag aacaaaagga gatgacacgg tgaaaagcgg taagatttat
     5521 gtaaccgccg caataggggc gggtatagtt tgcatagcgg caatgctggg tgtgttgagt
     5581 gcggctatat atgccgaaga tgaaaaaaat gacagctttt tagggctgat gtatcatcag
     5641 gtgctaaagg acgagagccg tgcgggtaaa tatataatta ctccttatga gcttgaagaa
     5701 gatctgaagt acctgtcgca aaacggatat gtttctatac tgccgtcaga gcttgaacag
     5761 ataaatgaca gcaagaaaaa gctgcccgaa aaggctgtga tgataacctt tgacgacggc
     5821 tatgaaaccg gtctgtatta tgttctgccg cttttggaaa aatatgatat gaaagcggtc
     5881 ataaatattg tcggtcgcta tacggacgaa tattcctgta tcaacgaaga gggaaagcac
     5941 ttgtcttatg cgtacctgac atggaacgag gtaaagaagc tgtccgacag cggctatgtt
     6001 gagataggca atcacaccta tgacctgcat tcaaacaccg gcgacagaaa cggctgtgca
     6061 caaaaggacg gcgaaaacac gtccgattat cgcaagactc tgtataatga tgtatccgcg
     6121 ctgtcggata agcttgaaga tgtgatcggt gtcagaccga gggcttttgc ctatcctttc
     6181 ggttctctta ccgatggaag ctcacaggtc atatccgggt gcgggataag ggtgtttatg
     6241 acctgccgtg aaataccgag taaaataaca ggcgggcaga taatagcagt taaccgctat
     6301 aaccgcgaaa gcggcagaag cgtacaggat atatgtgcac aatataacga tacgctgtaa
     6361 aaaatgacat agaaaaatca ggagcatcga caaattgccg atgctcccga aatttttccg
     6421 ttatttctga ttacttagac tcagaaacgt ttgttgagct gccaacgctt gaacctgatg
     6481 ttgtgttgtt gccgcaagct gttgccatag ctgctaccat agctactact gctactaaag
     6541 ctaaaatctt cttcattttt aaatctcctt acagtaagtt nnnnnnnnnn gtatcgatct
     6601 ataatgccga atctgtcgtt cgactgaatg aaattataca taaattttac gcggaggtca
     6661 agtgcctttt accttaattt gtttattact cttaattttc gttcagattg acaaaaaata
     6721 atgtaatcgt ttaaatgtaa ctggacattt tttagctgat agtgtataat aaatatgaaa
     6781 atcaaacgat tttgctgctt ttaagcaaaa tggttacgcg gttgtaactt tattggaaca
     6841 atatggatta tttgatttgc aaacggtacg aaagtataca taaattgtaa tcttttgaaa
     6901 aatattgcgc tgattttacc cggtgttaca gtcgcacacg ctgaaaacga gaatgtgaaa
     6961 ggaaaaattg ttatgtcaaa tgtaccaaca ccccatataa aagcgcagca gggagagatc
     7021 gcagagaaaa ttcttatgcc gggcgatccg ctgagagcga agtttatcgc agaaaactat
     7081 cttaccgatg ccgtttgcat aaacgacaca agaaatatgc tgggctacac cggactttat
     7141 aaaggcggaa gtattacggt aatgggaagc ggaatgggag tttcgtctat cgggatttac
     7201 agctatgagc tttataattt ttacaaagcc gacactatca taagaatagg cacggcggga
     7261 gcgcttgttc ccgaggttca gccgagggac atcatcattg cccagggagc ttgccatgac
     7321 acaaacgttg acagacagta cggcctgccg ggtacgtttg ccccgatagc cgatttcgga
     7381 ctggtgaaga gagcttatga gctggcgacc gagaaaaaaa taaaggctca tgtcggcaat
     7441 gtgcttaccg gcgaggtcta ttacgatgct tcaaacagcc tttcggcatg gtcaaagatg
     7501 ggtgttctgg ctgttgaaat ggaagcggca gcgctttata tgaatgccgc cgcggcaggt
     7561 aagaaggcgc tttgtata
//
LOCUS       DAWAOG010000022         7568 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1416_length_7568_cov_2.026887, whole genome shotgun sequence.
ACCESSION   DAWAOG010000022 DAWAOG010000000
VERSION     DAWAOG010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7568)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1416_length_7568_cov_2.026887"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2039..2048
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3164..3173
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6228..6237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2133 a         1595 c         1387 g         2423 t
ORIGIN      
        1 ttacacctcg tcttgttttc ttatttctgt tctaagtgat acgataactt ttttcagcct
       61 ttgattttca catttgagcc tgcggacctc ctttcttagt tcttctatct gaacagcaag
      121 agtatttatc tcgttggcag catcggcagc gtaacgattc cgtctgtcct cgattttctc
      181 tttccgaaga attgcccttg ctgtcatcat tgcttcctcg tcaagcacac gttcctctgc
      241 ggaattggct ttcatttttc ttgccagctc cgggtgtcgg cttaacattt tgtaaaactc
      301 aagcgttcgg aaattgttgt tcctgcaaaa ttcggagatg gaacaggatt tcttattctt
      361 tctcattgat ttgttgtcct ttcttttcaa gcgagtgctt gacaattgaa aaaaagcgtg
      421 tataatataa ttaaacacgt ttaataaggt gaggtgatac aatgtcagat aaaataaagc
      481 gtgaccttga agcaacaaga aatgctcttt taaattcaac tgcaaaactt atgacggatt
      541 gcagtgaacc ttcggaagtt acatctcgag ctattgcaaa agaaagcgga gtaaatcttg
      601 caatgattaa ttattgcttt ggttcacgag agggtttgct ttatgaggtg tttcgaaagc
      661 ttttgtcaga tgttcagaaa cacgatacgg aatttataaa gattatgtca tcgggaatgt
      721 cgcctaaaca gaagcttact gaattgcatt ttaaaatgat gaggcttatg attgcaaatt
      781 acaactattc gcaagcggtg acaaaatata tattgttcaa tcgcaatgat gattttggta
      841 tggaaagcct accatttatt gtggaacatt ttaacggaag aaaaaccata gaagattgca
      901 gattgattgc gtttgagctg acaagtatac acgaactttc agttctgcgt tatgagacaa
      961 taaaaagctc ctgtaatatc gacctcaccg atgataaaac acttaaatgg tatatttgtc
     1021 agaatataaa cagattttta gattaggaga aattaatatg tcaaacaaga aagcgcttat
     1081 agttgtagat atgcagaacg attatctttg gaatagaaga aaaaagatgt tttcctataa
     1141 cactcccgaa cttgtaaacg ctgtcaatag tcttattagc gagttcagtg aacggggtga
     1201 tgatgtgatt tatgttgggc aagtattccc caatattatc acaaataaat ggtttatagg
     1261 cttttctata aaaggtacat ctggtgctga aatttatcct gatgtggata tcgtatctga
     1321 taattacttt gaaaaaaatc tgcctaatag ttttacttcg cgaagcttca aaagctttgt
     1381 gactacaaaa gaatattcgg aaatcacagt atgcggactt gacctttgcg gttgtgtggg
     1441 agcaactgca aagggggcca ttaaaacagg aatggctgta aagctttcag aagcagctac
     1501 ggggtgtcgc ttcggaaaag aaaaagcaaa tagaatgaaa aaagatttga tttcattggg
     1561 tgtcaaaatt attaaataga tataaaagtg agccgtttta agcctgtgac gggtcattct
     1621 tcggcaaatc tcaaatacag ctgccacaag caaaagttca gccactttcc tcattccttt
     1681 caaaaatcca tatcatcata gcagcttcat acccatatcg tgttcaaagc ctgcaaaggt
     1741 ggagttcatc agcgtatata cggagtttaa cagcgatgtt gtcaggaagc ggtctatcct
     1801 gcttatcttg tcggggtaat tcagaaggct gtgcagaaca tactgaacgt gttcaaaggt
     1861 tatctttctg taagcagctt tcgccgtttc ctcggacaca cgtttcccct cggatattac
     1921 agtccgttct ccgcatagaa cgtccaccat caagtccttg atttcctccg aatcattaaa
     1981 atctctgttt ttcaaggaac ttatcagcat gtttatcagt tcctgtacga tcctagacnn
     2041 nnnnnnnnat cctagacggt tcaaacatat tcagatcaga taactgctta tctggtttca
     2101 atttctcaat agcattcaat tcgcgttttg ctatatcttc gtcctcccac actaaatatc
     2161 gcaaatgtga gcagatcaat tttaaaaatc tactgtcaaa tgatttagtg taaatgtcaa
     2221 cgtccttatt agccccactt ttattggtat atgaataaac gcgattgttt aagaatattt
     2281 ctgatacgct ttcataaatt attttgtaat taggaatatg cgaagcaacg ttttccagaa
     2341 gctccatcac catatcactg ccttttacga tctttctgac aagcacctcc caatgcgcct
     2401 ccgtagcatt gacccagaaa tcacgccaat cactgtagtg aaatgacgtg tcgcctttaa
     2461 aataataact tacgagacta tgatagacac tttggttaat gtgtggattg ttggaatgtg
     2521 catgacatgc cctgcaatta tcaacaaatc tgatgtgaga gctgtaggcg tatttgttat
     2581 tgacttttga atcttcgttt aggtaacatt tgagaaacgc cattccgcag tcatggtaca
     2641 gcacatacaa gcgtgtaaca tacagttcaa atgcctctcg aaatgaagat gggatcctat
     2701 tgatcattat atccgattcg cccaggaaga caaagctccg attacttgtc agcattttat
     2761 tgttcaaatc tcttgcagca catttttcca gaagcttagg tacaggaatt attttctcca
     2821 ctgctcacac ctccggatca ggatcaaaat ctcgcagttg cggaccacca agattgaacc
     2881 agatcatgaa atcctgatta atattcaaat tgtttcttct tatgtccctg taatttttga
     2941 tgctctcagg aacaaatatt ttcttctgcc cgacgatggc tgttttagtc ttattgttat
     3001 cgactatctc actgtcaatc tccgcagaca gcttgcccgg acggtttcct tctttgacgg
     3061 atacacgaaa acgccgcggt atgccttttt ctcccttctt ttcgggaccg cacaagcaga
     3121 tcaggcgaac cggactttca gaaaaatatc ggttcatatc ccannnnnnn nnngcataaa
     3181 tattcagtat gaaattcaag gaacgaatga taatgtcgtt atctcccgct tttgaaatcg
     3241 ccgttctgca gatctcagac atttcaaacc attcctcatt tttcaaagca ggatactgtt
     3301 cggtctccag cagaagatta ccggtataag acatccactt tgccttaaaa tacataagca
     3361 agcagcgata cgacatatcg tctggggtgg ggaattcgct tttcaggaca ttgatgatct
     3421 cttctgaggc tgttctcaga tccttcttta tattgcgaaa tacatccttg acggatcctt
     3481 tgaagccagt cgcatgataa tactcaaagt catcggcagc attttcattg agaaagaata
     3541 ttctaccaga caaatcacaa gcattgctat ccttgtctat gctttttctg tcaaacaatt
     3601 cgggtttttt ttccttatat ttcagcagta gcttgacaag aatctgcttt acacggaagt
     3661 atgcgtacga gtcattattg gggttgctga aatattcacg cagttcggcg acacttctct
     3721 cattgacttt ttcatagatc ttgttgatat tggagagcac gtttacatag tcatcgctgt
     3781 catcaattag gcttgattca ttatagaaca gcagatcgat atactcaagc gtatcgggga
     3841 tcatataatc atcgcaaaat tgaatggata tcacataatt cagccagatg tccagcagta
     3901 gggtaagaga aaagctgttg ttctgcttgg attctatgcc gtttttcact gcaaactcaa
     3961 aatatccctc gaactcatca gacagttctt tgattttttc ttcgtctctt gtatcatcat
     4021 atatcttcat ctgctggagc agatttctgc atatttctcc gtaaagcctg gaaagagtcg
     4081 gttttgcatg acactgctct gccgcgtctt tcattctgct gatagcatcc tgcagaaaat
     4141 caatggtgtt atctctgccg ttagtttcga tataatctct ggtaaaatga cccgagatca
     4201 gcatagcctc cggattgctt gacgcatact tattgaggca ctccacaatc tgcggccaga
     4261 attcggaata ttctctgtat tgtccgcgag acacggattg atcgtttacc tttccgtatg
     4321 aattagttcc gaatgaacgc acaaaagccg caacagcttg cgcttcctca tagttatccc
     4381 agcggcaagt ctcgataaga cactttacaa cttcgatctc tttgtacttt ctatccttgc
     4441 cgtaattata atccagataa gctatagcct cgctgggatg tctgaatgaa accgtgatat
     4501 taccggttga tgaattgcta tcgtattccg ccatactgtc ggtgcgcagg atcctgttca
     4561 gcttgttata gtctccggtg tactgttttc cttcggaaat ggcacgcatc agcacagaca
     4621 gcggtaattt gatggatttt ttgaactgat ttgccacagc gaggatcagg ttcatttgac
     4681 gaatcgcgtt gatcaaggga acgttacgtt tttgacgttc agaatctata tccataaatt
     4741 tcgattgtac tgaagagcca ataccgagac tgaaaacagt cttttttaca tcgttgaaac
     4801 tctgcacatt cttatcctta aatattttga acaggtcgtt tgagacattc tcggaggcct
     4861 tggcttcctt gtacaagcct gcgttaagga cattcctgac cttttcccat tctggtgagt
     4921 attgatactt tgcaaaattg atgagctttt caaaaaggta atcattgctt gaatttacac
     4981 ttccgctttg actacgccag ttcttgttga ttttatccaa gagagcgtca agcgctattt
     5041 tcgccttttt ttcaagcgtt gtatgcagcg gtttcagttc attgaaggca acaccctgta
     5101 caacagaaga atcgacatta acagattcaa ataaatagat actgccgatc agaacggcat
     5161 tacactcgtt aagcttgtta tacagatcga tgtaatcagt ctttttcttg gtgctgctat
     5221 agttatccca tataattata acatttctta tacggtctcc cttggtggtc cgttgagcat
     5281 agacatagtt cttaataaat tctatcagac tatcctgcca gtcgctttca cggttcgtag
     5341 gatcgccgct gataaaaagt acgatatact tgttgttttt tcctttagcg atgccacccc
     5401 ttgccgatgc ggcataccag gcaaaccaac tgagtgatgt agtcttacca ctgttggaat
     5461 ttcccttcaa aagtatgatc tcgcgcattt tattcttaga tttcagctgc tctgacacgg
     5521 tatttttcag atagtcttct acatcgcgcg ggacatggaa actcattttt tccttattgt
     5581 agatgatatt aaaataattc caacttttct tatcacgctg ctgaataaaa tccgcaaaag
     5641 ccctgtcctt atttttgtca tcaagtaagt aaggagtaaa gatctcatct ctcataagag
     5701 tgatacctat gctgtttaag cgggaacatt ccttacgcgg gatccatatc gtgtcgttgt
     5761 tcggcaagct gatgcgaacc tcgttctcat aattctcttc ctcgtcttcc tcgtcctcat
     5821 ctgcaagccc gacctcagta acggcatcct tcaggctcat ttcacacaag acgatctgac
     5881 cgttgtcaat atactcctcc aacagcatcc agtcatcttc gttcttgctc ttgcatattt
     5941 gctctgcctt ttgcttatcc atgccgaata tatagataca gccattcgga actttgtcaa
     6001 aattgctaag caatctgccg atatcaaacc agtcatcctc cgtcaatccg tcgatcacaa
     6061 gataacccca tacaaatagt atctgattaa agatcatgtc gatgaacccg gtcttatttt
     6121 tcagtttcaa gaggttcttt ctattctccg ggatagaatt ttcatctcca aaaagagaaa
     6181 taaaatgagg ctgttgttta ttgaaatatt tataaaacta tgaggatnnn nnnnnnnttg
     6241 cctgtcagtt cttcttgcgt ctggatattt aagctaaaat tgttgcctat actgtttttg
     6301 aaccaaagat tattaggtga tgaagaaaga ataatattcc acctgagtga gagtatcgct
     6361 ctcagccacg gctgatatga ggcatgctcc gcagcggaca ccatcgcctt gcccaaggta
     6421 tcgggagcct tttcccaaag actgttcaga ctcggcgttt catttttgat atccaactgt
     6481 ttcagaacag acgaatagta cggatcaacc ttaaagtatt cctgtccaag cagcaaggcc
     6541 gacttgccat ctctgatatc ataaaataag ttgttaatct gcttaaggct taatgacata
     6601 aaaataaacc tccctgtcac attaaaaatg tccaatcaat tactctataa ttaaattaga
     6661 tctatatcaa tatgtttgat cgtataacaa taaacgttag cgctagccaa tggatataac
     6721 ggaaaaacaa aatgtttcag cttttgacaa cgcaatgata ggcttcttca tttactaacg
     6781 attaaaatga aacatttaaa gagtaaataa gaaagccctt gtcgctcact ccaaatgagc
     6841 cgtacgatac ttcaatcatc atttaacatt gagattaact tttctccgtg tatattatac
     6901 atcattatta ggaaaaatgc aatcagtttt tttaataaaa tagacaactt attattgggc
     6961 aattgtttat tttattgtga aacgctgagg acaaaaattt tcgactttag ttaaagcggc
     7021 ttttcaggtt ctcgtaaaaa ggctatttcg caatgctcat tattatgcgg attattaaat
     7081 gaagctaata tattattttt gcttggtttt gcgcttttga acagcttcaa atatttctct
     7141 ctcgataatt gcctcatgat cattctcaat gaaatacatc gacaactccc cctcgttctt
     7201 actacgcttc ccagttaaga agtcctcggt aaagctcttt tgcataagaa catcaccgac
     7261 atacttttca ttggagagga ttcggtcgat tgttgaagtg ctccaaacat ctttgcctgt
     7321 cacggtctta attccgtttt cctcaaggaa cttctttatc ctacgaacac caaagccttt
     7381 gagatataac tcgaatatta ttctgacaat aacagcgtcg gactcaacaa cgacaagcct
     7441 accattttca tctttatcgt atcccaagaa ccgtgtacag tttaggcata ctttaccctc
     7501 gcgcattctt tcccgaatac cccacttgat atggtcgccc atgttcttgc tttcttcctg
     7561 tgcaaatc
//
LOCUS       DAWAOG010000023         7560 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1417_length_7560_cov_2.431179, whole genome shotgun sequence.
ACCESSION   DAWAOG010000023 DAWAOG010000000
VERSION     DAWAOG010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7560)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7560
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1417_length_7560_cov_2.431179"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2986..2995
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2048 a         1802 c         1584 g         2116 t
ORIGIN      
        1 cgcaaagaca gagcttcttg taacagcgct cagccctttg accgcaccgt atacagccga
       61 tgctgttatc accgctattc ccgatattgt tacaatattg ttcagaagcg tatctgtaac
      121 atagcgctgt acgctcaata cggtctgcac agctgtggcg gaaagaagca ccgacataat
      181 tatccctgcg gttataccga atgcccgatt tctgcgaaac gccgctgtta tcacgctgtc
      241 gcctttgaat ttcagcgtta tgaatataac cggcaggtac agcaatgcgg caatcagttt
      301 tgcaaacagt attaccgcaa atctgtcgaa gccgtagctt accatctctc tggggatcgt
      361 tatcatttcg gacgataatc tgcacgccag aaaggctatc acaagctgtc cgttacttat
      421 cctgctcatc tctgattgtc ctttcaccgc catgtttgct cttagccttg ccggacaact
      481 gctgtaccgt catatccgag ccggaaaggc gtttccagct tgctctgaaa aaggtatccc
      541 gcataatgct tttaatcgtt acaggtgcga aaggtgcggt atatggcact ccgtatgcgt
      601 tcttttcgca tatcctgaaa aatatgacag tcccgcatat ggttatcccg aacagcccga
      661 gcattccgcc cgcaacaata aacacaagcc ttaatatcat acagctgtcg tacagatccg
      721 gcacggcaaa cgagcacaat ccggtcgcgg ctattatcag cactaccggt gcgctcataa
      781 gtcctgcaga aaccgatatt tcgcctatta caagtccgcc gactatacta accgcgtgac
      841 ctacacttct cggcagcctt aatcccgcct cacgcattat ctcatacagg atatacatta
      901 tcagacactc gccgaaaacc ggataagccg ttgtctgaac tgatgagtag atgttgagca
      961 aaagcccggt acggaacatt tcagggtgga agttacacag tgcgacataa agtccgggga
     1021 gtacagccgc cgcaataaat gcaaaaaacc ttatagtacg cagaaatgcc gaaaagtatg
     1081 acttatgcga atagtcatcc attgttacaa agttctctgc aaaaaggcaa ggcaaaacaa
     1141 gcgcaaacgg cgttccgtcc actattatcc ctactctgcc ctcatatagc tttgacacaa
     1201 acacatcggg acgttcggtg acggatattt gtgaaaacag cgtactgccg ccgggctcta
     1261 aaaagctttg cagatactca ctgcttacta tggtgttaag cgggatattc ttaagtttgt
     1321 caataacggc gtttacagtg ttcacatccg ccttgtcggt aagatagcac acagaaacgt
     1381 ccgtattgct acggtcaccg atcgtcatca taacggtttt gagtgtgggg gatttcatac
     1441 ggcggcgcac catagacaca tttgttctaa ccacctctat aaacccttcg cgggaagctc
     1501 ttaaattcgt atgagtagac ggttcgtcta tgcttcggtg cgaatatccc tgtattccta
     1561 tcgctattcc gaacgaagcg ccgttacaaa gcaaaacggc aaatcccgac attatataaa
     1621 ggcagagatc tccgatatca tacatctgct tttgctcctc ggctataggc atatcggtgc
     1681 atactgcaca gaaaaagccg ttatcgtccg ctatttcggg aggagatgaa acaagcgggc
     1741 ggaatataag gtttgcaagg tcagccttgc ctatcatgcc ctcacaggta agcaatgcca
     1801 ttttatgacc gcctacggta aaatatttga ttataagatc cgagctgttg ttcataagca
     1861 ctcttacagc acagcagttt tcatcaagat ctgacgacag tgcggtatcg gatctgttta
     1921 cgggttcggt gttcttttcg gaaaaatccg gcttttttat attgctcatc acaagtcctc
     1981 gcataataaa aagtaatctg taaataatag cattgaaata aaatctatca attatctttc
     2041 ccgactatgg aggcaatatg ctgataataa tcattcgttc tgtacttttg tacattctta
     2101 tagtgtttgc gctcaggctt atgggcaaaa agcagctggc acagcttcag ccgtccgagc
     2161 ttgtaacgac aatacttata tccaatattg caacactgtc gctcgaggat tcaacagtac
     2221 cgatgatata cggcgttata ccgatactga tgatagtttg tcttgatgtg ttcatgtcat
     2281 ttattatgct gaaaagtcag cgctttcgtc ggcttgtcac cggaacaccg cagataatca
     2341 ttacaaacgg agttattgac cgcagagtga tgaagcacct gcgctacagc gttgacgagc
     2401 ttatggaggc aatgcgcgaa tgtatgatat tcgacatttc tcaggtacaa tacgctatag
     2461 tcgaaaccac cggcaagatc aacttctacc agaaaagctg ctacagaaac gtcgaaaacg
     2521 gtgatatgga aatcaaggtg cagaactgcg atccgccttg tctgcttatc aaagacggaa
     2581 agatcaacta tccgggtctg cgccgctgga acggtgacga gaaaaagctt cgcgatatga
     2641 taaaggatat gaggcttgat ataaaggata tattcctgct cacagacagt gtggacaagg
     2701 gcgtttacac cgtgcttaag tcggataaca aaatcggcac aaaatcaatg tgcatgcctc
     2761 aggagaaaca gtaatgggaa gaatggtaac ttgcgccgta atagttgcgg ctatggcagc
     2821 atttgcaata ttcggataca actactccga taaccgtgct aaagaaatat atatcacagc
     2881 cgatgagata acacaggcta tagataatga caacaaacaa aaagccgccg aaatggcggc
     2941 ttatgccgag gatatctggc gagagctttc acaggatact atcttnnnnn nnnnnataac
     3001 gaaatagcta tgtgtctgtc acggataagg gtatttgcag agaacggaga ggacgatatc
     3061 tattcggaat gtgcagtcct taaaaggctt atagagctat atataacaag gcagcagata
     3121 acgctttcaa gtgtgctgta atatacaaaa aatgccgacg gcaaaatacc gtcggcataa
     3181 tttttcaaat catcattcaa agcttaggaa acagtagcat tgagttcctt tattcttgct
     3241 tcgataacgc ctctgttttc ttcacgaaca gttgcaaatg agctaccgcc cattacaacg
     3301 tctgacgtta ggtgacctac agggttctca cctgttgaag agtcagcaat atcagcaccg
     3361 ataccgttct tgaactcgaa tactgccgag aacgttgtag gatccttcag cttatcaagt
     3421 ctgtcaagca gtgtgtctgt ccagtcaaac tcatctatag actgctgtct gcctgccttc
     3481 tttacttctt catccttgga agctaagagg ttagcataga tccatgcttt ataaccgtca
     3541 gcgttcttag aacctgaaac gaacataaat gcatcctgct tcatttcctg ataatatgta
     3601 tcagcaccgt ccatctgtgg gaaaggaacg aagttgatct catcatcatc ccacttctgc
     3661 tttttcttat acagtctcca tgtttcctca tatctccatg taccgtcttc aaggaagaga
     3721 gtgttaccgt ttacccactc gggatagctg ggtgcccagt tgttttcgat ctctctgggg
     3781 taacggagct gttcctgtgt atcatcaaat gtgcggagca tatccatgca cttttcaata
     3841 ttggcatcgt taaggttgct tacaagcttg ccgctctcaa gaccgatgag tggcttacct
     3901 gttgtagcga tgaatgcgtg gtcaagtccg taaccgtcaa atgcatactt ggcattatcg
     3961 ggatctgtga actttcttgc catctctatg cactttgacc atgtccattc acccttttca
     4021 aagagctccc agggatcgtc aaagccgttc tcttcgatta ttgactttct gtaccagaga
     4081 agtgttgcgg ggtttacatc aacgataggt gtgtagacct tgccgcccca gttgaacata
     4141 tcgataatat tcttatagtc tgcccagtca tctgtcgtgg tatcaataac gccatcgata
     4201 ggctggaaca ggttctggta aataccatag ggatagttag atgtttcaaa ggggaatgta
     4261 tcgggagaat catccgactg tacgagcttt gcgagatcaa catatctgtt tgcatagttt
     4321 gatactctta cgttaacgaa tacgttttcg tctgctacac tctcatagcc ttcgggcttg
     4381 ttcttgggtg tgccgtacat cgatttgaag agttctacgg aaggagctgc ctcctgaatg
     4441 tcataccatg ccataaactt aagcttggtg tccaccttga tattcttgta gtcctctgct
     4501 accgaaacgt tgtcgatcgc actcttgaca tcttcgtttg aagacattat cttctggctg
     4561 cctgtagctg ttgtgctctc tgcagaagaa tcagcaccgc ctgctgttcc gccgcttgtt
     4621 gctgtaccgc ctgaacaacc tgcgaacgct gtagcgccca ttaccagagc aagagctgct
     4681 gctgaaatct ttttggtcgt ttttttcatc atttacctcc tgtacggctt tacattgccg
     4741 cttaatcacc ctattattat aacaaatttt caggtttcaa tcaaccatta gttaaaccaa
     4801 acttaaaaaa tatttttgtt taattatgac aacgaattat gcgtttacac ggtgttttca
     4861 caaaatttaa gttaattatc tttatacatc tgagaataac ctaattgttt aaaaccgttt
     4921 ctcgcgtata gctttgccgc tctttcgttt tcaggctcgg tttcgagtcg tattcttgca
     4981 acattggcat attccttttc gatgaatttg aagaactctc cggttatgcc cttacagcga
     5041 tgctccggct ttacatatat ctcctcgagc cacaccacaa taccgcctgc ctcgtttgaa
     5101 taggtaagtg caagcagagc aaagccgtaa acctcttttt tatcgtcact gcggaacata
     5161 aagcagaccg tatacggtga gccttccata agcacatcga atgtaagctt atgattttca
     5221 tcgggaatag gatgaagcac agcgggagaa tgatagaaat catgctccat agcaagatat
     5281 tcttcacggt cggtttttct tattttttca aataccatat tacctcctgt catgcaagac
     5341 ctaacgggtt aataaacaga tacgcaaaat atcctgcgta agcaacgagc attataatgc
     5401 cgccgactct cgtaagctct tttttacgca ggacaagcag acccagcagt acagcagtag
     5461 ctatcgcaac tatagtatca atgataaatg cctcaccgaa ggtcagtacg ctcggggaga
     5521 atattgatgc cagacctact acgaacaaga tattaaaaat gttactgccg attatattgc
     5581 ctatagcaat atcggatttc ttcttgagcg aagccgcaat gcaggttata agctcaggca
     5641 gcgatgtacc gaatgctaca acggtcagcg atatggtcct gtcatccact ccgattacct
     5701 gagctatgct ctttgctgac gaaaccgtaa ggtcgcttcc gagtacgatg ctcacaagtc
     5761 ctataagagt gataagtatc agaacaggta tcttgtcctt ttcggtaagc tgcggcactt
     5821 catctgccga gctgtcgtca cccttcttgt aaagatagaa cagatatcct aagaatgccg
     5881 caaaaagcac cagcatgata acaccgtcta tcttgtcaag acctccgcct atccagccga
     5941 gaagcagtaa tacagcggtt ataaacacaa caaacggtat ctcgaattta agtgtgttcg
     6001 gctttatctt aaggtttgtt atcagagcgc tgacacctag tataagcagt atattcataa
     6061 tattactgcc gactacgttt cctatcgaaa tagccgccgc atcctgaaat gccgcactga
     6121 tactgattgc cgcctcggga gcggacgtac ccattgcaac tattgtaaga cctattacta
     6181 tcagcggaat tttaaattta gccgcaattt ttgacgcacc ctcaacaaac agatccgcac
     6241 cctttatcag cattacaaag ccgactatga gcactccgag cgagcatata acatttgcat
     6301 tgatattcac gccgaccatg ttcatcacat acagtgccgc tccgatcaca agcgcgataa
     6361 ttgctattaa catttttgtc ctctttcttg tttttcaact taattttttg tctttacacg
     6421 aatgacgggc agtaacgttc atgagaagcg actgcccgta catatatcat tttattaact
     6481 tactgctgac ccacaagctc ttcataaaga cctatgtaca gcttagccga ctgcttccag
     6541 ctgaagtctg ccttcatcgc tcttgtaacg agcttgttcc actcagtctt gttccagtag
     6601 cagtcctttg ctctcttgat agcataaagc atatcgtgag cgttgtacga ctggaatgtg
     6661 tagcctgtgc cgttctcatc accgcaatca attatgctgt ctgcaagacc gcccgttttt
     6721 cttactatcg ggatagtgcc gtaacgcagg gatatcatct gtgcaagtcc gcacggctcg
     6781 ctcttgctgg gcataaggaa catatttgca ccggcatata tcttcttggc aagatcgggg
     6841 atatatccgc aggtaaatgc tactctgtcg ggatatttcc agcgcatctc gtggaagaac
     6901 tgttcatact gagcctcgcc cgagcctaat ataacgacct tatagccaag atttatgata
     6961 tcgtcaaata cacactttac aaggtcaaag cccttgtgct caacaagtct tgataccata
     7021 ccgagaacag gctcatcgcc ctgaggcagt ccgacctctt caagcagctt ctgagtgcag
     7081 gttttctttc ctgctttctt tgtaacggta aagtttgctg ggatagcggg atctgttgcg
     7141 ggattataca tatcggtatc gataccgttg aggaaaccgc aggtcttgta ctgcttgtcc
     7201 gcaagcgctc tgtcaagacc gtgcgagaac cacggatcaa gtatttctct tgcatatgtg
     7261 ggagaaacgg tcgtgacctt atccgaaacc tctatcgccg ctttcataag gttgatatct
     7321 ctgtcatatt ccataagcga ggcgaaatgc ttcggtatgc cgagaacgtc ttcaagtatc
     7381 tccataccgt atctgccctg gtactgaata ttgtggatag tgaacacatt cttgatgccc
     7441 cagagcttct cgtcatttct gtagaaaata ctgtaataaa cggggatcaa tgcgcactgc
     7501 cagtcgttag aattgattat ctgcggcttg aaatcgatga ccttgagcat ctcaagaacc
//
LOCUS       DAWAOG010000024         7435 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1442_length_7435_cov_2.718970, whole genome shotgun sequence.
ACCESSION   DAWAOG010000024 DAWAOG010000000
VERSION     DAWAOG010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7435)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1442_length_7435_cov_2.718970"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    615..624
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2184..2193
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4986..4995
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6196..6205
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2113 a         1491 c         1756 g         2035 t
ORIGIN      
        1 agcaagggca gtcacagttt ttttgccatg accgccatat cattttcttt attactctgt
       61 gtgaccccgc cgttacgcta tctgttgcct atcgacaata tatccaaagg cggaattgtc
      121 agcggcgacc cgccgcatta gccgttgccg acttctacaa catagatatc aacatactgg
      181 agtccccatg cacaagcatc atcatagtgc tcttcggtac tgcccatata tacatcaaca
      241 gggatcgttc ctgccatcat accctcgcct gtatctgccg ctatagcata gccgtagcag
      301 atgttgtcgt actgaccgac aatgtacagc tttgagccat aagggatcac atcgggattt
      361 accgcaactg tgcctatctc ggcaagtctg ccgctggctg tgtatgctcc cgcgggagct
      421 gtgtaggctg tagctttgcc tgatactaca tactcatagt caacaggtct gccgttctta
      481 agtacgatag atgtatcatc aattttgcaa taaggagtta cgatcgccgc accctcggct
      541 ataacctcgt caaccttctt tgtaactacc tttgttgtct tcttgacatc ggtctttacg
      601 ccgtcgatgt aagtnnnnnn nnnncccttt gtgcgaacct cgctctgacc tatagccaga
      661 atgttggtcg taaccgttga ggtctgataa gggatcgtct tatcctgcgt ttcggtcttg
      721 atctcgactc ttgaaacctt tatctcggta ggctcggcga tctttgtttc aagctcctcg
      781 cttaccatat cgttcttgcc gagcgttact ccggctcttt tcaaaagctc ggaaaccgtg
      841 ttatcaagac actttacggt agttgtctta ccatcggcag ttaccttgac atcatacgct
      901 cttgtgatcg ctatcttctg accgtcttca agaaccgtgt ccttggaagg tgataccttg
      961 tcgtacttgc cgagctcaac gcctgcctgt tcaagaagct gtgctaccgt ggcatcaacg
     1021 ctgtgcactg tagctgtctt ctttccgtcc tcgagtacgt cggcatcaaa cgctctctca
     1081 atagtgagag tagcagttgc cacgttttcc tcgaagccgg taaactcaat cctgtcataa
     1141 gcgcccagag tgatatggtt ctctctgagt atctcgtaag gatcgtcact taccgtataa
     1201 acaatacgct tttcgttctc gtctattatc tgtacatcat ttctgaaata gagcagacct
     1261 gtcatcagga atgctccgaa gatactgccg aatatcagca gtattttaag cgctgcgttc
     1321 tttgatatgc gtattgtcag tccggtgttt ctgaatctgg gaaacatata tccgcctttc
     1381 gtcctcatat ctgtaggttt aagcagtacc catatgggca tcacggcatt aaatttacgg
     1441 ctatatccgt ctgtcaccac tgaccgtgta cgataagccc catacgggga aaaccgctgc
     1501 tacgtttatt attgggcttt tttcaagctc tagacaatta taatttccgt tttgcaaaat
     1561 gtcaaatagt ttcggtcagt catttttgtt gaaaatgcac aacaaatttt tgttaccgtt
     1621 ttgtaacctt tttgttaccg ttttgtaatc agtaaattat tggattatgt tgactgattt
     1681 tgtaatttaa tttgttaatc tgcccaaatt tgaagtttgg attttcattg tgtttttgca
     1741 caaatataat cgactttttc gtgtagtttt acagcgatta taatatgaaa tggtaattta
     1801 gtttcacggc agaaactgac aaaacggctt tgtatacagc aaaaatgccg tttattgcgt
     1861 gtctgtaggg tgactgagct gtgaatgtaa tcgtatactt gcaagacgat ttagggtcga
     1921 ttttatgtcg ggagcgtgtc ctttatatat tataatagta tagtcggttt ttgcggttca
     1981 aacggtggtt gattttgcgg cgtttttggt atgaacaatg gtacataaaa aatacagcct
     2041 ccggttcgag gctgtattcg ggtcatacta tatataatat aaagggtgga tcagcgtatg
     2101 gaggttttct ccgttgaagc gtttatattt gcaatgcaaa tattttgcct gctattatat
     2161 tagttgtttt tccagctgac aatnnnnnnn nnncaatttc tgctgagagt tgtttgtcaa
     2221 ggtcgacacg catagcttta cattcggcgg ggaatttttt agccgcggag aagctcatct
     2281 ttacaagcag atccgcaccg acaggcagag cctttttcag cacgctttcg ggaaacacaa
     2341 aatgtgtgcc gtgctcatag aggtaaatcg agtatttgca ggtatggggg cgggacttca
     2401 ggcgctcgtc cattcggcgt atgtactttg cggtatccca gagggagtcg tcttccgctc
     2461 ctacaagaac aagcttgccg gtgatgttct ctaccgggat aaaatgctct tccttaagca
     2521 gtccggcggc ttcggaatcg tcaaaaatgt cttttgaata tgtcgagtca ccatgctcct
     2581 tagacgcttt ggctattgtc tgccagtact cggggtgctt atagacaaag ggcatatagg
     2641 gtacgggctt gccgccgaca gacacgatgg attcgttctc gataggccac tccttacagc
     2701 cgtcaagatc gccctgggca aatccctgcc atacaaagtc gctcggtgta agggctagag
     2761 taagtgtgat gtcgtgatag cgagaggcgg cgataagcgc aacggtggcg gttgtagacg
     2821 cgccgactat gcctatcttc ttgttgccgt gggctttgag ccatttcaca gtcgcttcta
     2881 tacgctcgag gggatagtta tgatggctgt aattcttctt accgggggac attgacataa
     2941 cgttaattcc ataggtgtga agccacttgg cacatagcgg tatcggacgg ggttttacac
     3001 tcccggtatg cgccgtaaaa gccatcggtt tcgggttcaa aatgcagatg tttcatagtt
     3061 tactccgttt ctctttggtt agttttgcct aacgcaagta tagcaccaaa caggcagaat
     3121 gtcaagaaag aacggaatgc aggcacaatt ttacattgtc ggatcttaca cagcattaag
     3181 cccggcggca aattgcttga caaacaataa gcacaatgat atactttaag tacagttaag
     3241 cattaagttg cagtttatta tccgtgggcg atattttgat atcaatggcg gttacatatt
     3301 gatatcaggc acggctcgcg gagggttgtg cctttttgcg ttttagattt aagtataagg
     3361 agaggtctta tgaaagtgaa aaagctgact gccgggttac ttgcggttgc gatcacggta
     3421 agcttgatag cgccggaagc atttgcggaa attagcggtg ctaattctgt cggaaaggtt
     3481 actgttagcg ggctaacagt cgatacaccg ataaagtccg cgtccggtga tacaggtgaa
     3541 acaaaagaac caagcgaacc gagcgaacca agcgaggacg aaagcgtcta tacatacggt
     3601 gtgcttggcg atgggtctat agagattttt ggctataaag gtagtggaga gaatattgtt
     3661 attcccgcgg aaattgatgg gaagagtgtg acaatgattg gcagttatgc tttttcagat
     3721 cacactgagc ttacaagtat aacgataccg aatagcgtaa agagcataga cgatggcgct
     3781 ttttttcggt gctatcacct taaaagcata ataataccag acagtgtgac gagtataggc
     3841 aattttgcat tctgcggttg ctcagactta gcaagtataa cgataccgga tggtgtaaca
     3901 agtataggca accgtacttt tatgtggtgc agcagccttg aaagtataat gataccagac
     3961 ggtgtaaaaa gcataggtac agatgctttc aacagttgca aaagtgtttc gggtataacg
     4021 ataccgaaaa atgtgacaga gataggctta agggcttttg cagattgcgc aagtcttaca
     4081 gaaataaagg tttcagatga aaattcaaat tatgcttctg tggacggagt tctgtacaat
     4141 aaagataaaa cttctgttgt gtgttatccc gcaggaataa aggatagcaa ttataagatc
     4201 attgacggtg tgacgactat agaacacgaa tctttttatg attgtaccga actgacaagt
     4261 gttactgtgc ccgacagcgt gacttgtata tctggcgaag cattcaagag ctgtactaat
     4321 ctcacaagca taacattacc tgacagcgca atgtatatag acaactgggc attctataac
     4381 tgtacatcac tcaaagaagt tacaattcct gcaagtgtga cagctatcgg cgatgaagct
     4441 ttcggatatt gctatgatgg agatagctcg gaaacaaaga aggtcgatgg cttcaagata
     4501 aattatgcca agaatacata tggacactta tacgcggtta aaaacggttt ttccgatgaa
     4561 atatacctag ttaccaatga gcttgatgat ggtacgttga caatagccaa atacgcagga
     4621 aacagtgcga cttatgaaat ccccgctgaa attgacggca agaaggttac agatatttgc
     4681 aacggcgcat tcagcgactg caccgagctg acaaatgtaa cgataccaga tggtgtaaag
     4741 catataggat acgatgcgtt ttataactgt acatcgcttg aaacggttac aattcctgca
     4801 agtgtgacat atatttacga taaagcattc ttaaattgta catcgcttgc ggcagtttcc
     4861 ttgccggcaa gtgtgatatc tattggagat aaagcttttg gctattacca gggaaatacc
     4921 gctttccttg atagcgatag gagcgtgttc acgggaagaa ccgcagccga agttgaatcc
     4981 ggcaannnnn nnnnngatga taactatgaa ataaaggcag ttgatggatt taagataaac
     5041 tatatcaaga atacatgggg tcattggtat gcaaccagaa acggtttttc cgatgaaact
     5101 tgtcttgtta cctccgagct ttataacggt acattgcaaa tagatggata tgccggaaac
     5161 agtgcgacat atgtaattcc tgatgaaatt gacggcaaga cagttacgca aatcgttaac
     5221 aatgcattca atgactgcac atcactcaaa gaggttacaa ttcctgcaag cgtgacagat
     5281 atcggttatg gagctttcgg atattactat gatcgcgagt gcatggaaac aaagaaggtt
     5341 gacggcttca agataaacta cgtcaagaat acatggggac atctgtacgc tgttcagaac
     5401 ggatttaccg atgaagcttg tcttattact acagagcttg atgacggaac aatagaaata
     5461 agcaaatatt acggaactaa cgaaacatac atcattccgc ccgaaatcaa cggcaagaaa
     5521 gtaacggaga taggcgaaca ggcatttagg aataatactg cacttaccaa cgtaaccata
     5581 ccggacactg tgacaagaat aggacggctg acattctgtg gctgtaaagc attgacgagt
     5641 ataaagatcc cggatagtgt tatcgaaatt gaggattttg cattcgagga gtgtgattct
     5701 cttaagaatg tttcgatccc ggcaagcgtt accagcatcg gtatgtgcgc ttttggctat
     5761 acatatgacg agggcattaa ccgtaaaaag gttgatggtt tcacactgga atatgtaaaa
     5821 aatacggcag gacatttgta tgcggcactt aacgacttta ccgatgaaag ctattttgtc
     5881 acagaagagc ttgaagacgg aacattgtca ataagcagat actacgcaaa agatgaaaca
     5941 tatacaattc caccagaaat aaacggtaag aaagtaacgc agataaaaca atatgctttt
     6001 gcacgcaaca agtatctgaa aagcattgta atatccgaaa atgtaaaaga aataggacag
     6061 catgctttta gcggttgtac tgcgcttacc gatgtgagta taccggatag cgttacaaaa
     6121 atcgatgcac gagcttttgt taattgtaca tctattctga atgtttgtgt acctgcaaat
     6181 gttacttcta taggannnnn nnnnngagag caagctttcg gatatgagta cgatgattta
     6241 gggtatgaag gaaaaatcaa aggctttaat ataaaatgct ataaaggcac agcgggcgaa
     6301 cagtatgcaa aagataatgg ctttgattat gaaattcttg acagtgaaac gcagtgtact
     6361 cacagcaaca ctgagatacg taatgcagta agtgcggatt gcacgacaga aggttacaca
     6421 ggcgatacat attgtgctga ttgtgacgag ctgatttcaa gcggcgaggt tatacctgcg
     6481 acaggacata cagaagtgat cgacaaggcg gtagctgcaa cctgtaccga aaccggtctt
     6541 acagagggca agcattgctc cgtatgcaac actgtaatta aggcgcagga agttgtacct
     6601 gctaagggac ataagtacgt caacactgtt gtaaagccca cctacacagc aaagggctac
     6661 acacttcaca agtgcttagt ttgcggaaca agctataagg atacttacac ggctaagctc
     6721 acacttgcaa aggttacagg cgttaagctt gcaggtaggg cggctgacgc actgagaatt
     6781 aactggacaa agaatgcaaa tgccgacgga tatatcgttg agatgtatca gggcaacaag
     6841 tgggtgcgtg tcggcaagat tacgaataac agcacaacta ctttcagaaa agcgggactt
     6901 aaggcgggca ctgcttacaa gttcagagtt aaggcataca agatgtatga ccgtactgcg
     6961 gtttacggcg catacagtgc aactgtgtcg gctaggacaa acccgtccgt tataaagggt
     7021 gcaaagctcg gcggcagagc cgcagacgca ctgcgtatca actggagtaa gaatgcttcg
     7081 gctgacggat atattgttga gatgtatcag aatggtaagt ggacgcgtgt tgcgaagata
     7141 acaagcaaca gcactactac tttcagaaaa gcaggactta aggcttcgac tgtatataag
     7201 ttcagagtca gggcttataa gatgagcgga aagacggcgc tttatggtaa ctatagcgct
     7261 actgttacgg caaggacaaa tccgtcagta atgaaaggtg tcaagatagc gggtaaggct
     7321 aaggacgcac tcagagtaaa ctggactaag aatacttccg cgcaagggta tatcgttgag
     7381 atgtacaagg gcggaaagtg ggtaagagtt gctaagatca caaacggcaa cacga
//
LOCUS       DAWAOG010000025         7326 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1462_length_7326_cov_2.501444, whole genome shotgun sequence.
ACCESSION   DAWAOG010000025 DAWAOG010000000
VERSION     DAWAOG010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1462_length_7326_cov_2.501444"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4992..5001
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1924 a         1602 c         1673 g         2117 t
ORIGIN      
        1 gaagagaaag taatcactgt taagtgagta tttacttaaa ttaagtacac tatcccctcc
       61 ggaaacggag gggatttttg tagtaaaaac agcttacaat tatgtttaat ggtgcggaaa
      121 tcgaagaaaa gcggcattta ttatattgtt tcaccaaatc gaaaaaagtt ttttgtgaaa
      181 attgccaatt gcaaaacatc cggcaatata gtatgattat aaatagctaa tgagctaaca
      241 aaattttatg gaggacaaaa aaatgaagtt tacaaaaact ttagctgcaa tcgcagcagc
      301 ttcactcgca gtatcatcac ttgcagtaag cgcttttgca gcagcagcta tcacatcaaa
      361 cagtgctatt gatctcgctt cagacaacaa ggctgacgca gtaatcaagc tgtacgaaga
      421 agacaaggag cacaagcccg ttattgatct gttcacagaa aagaacattg ctcgttctga
      481 cgtttacggt gttaagcttt attttgatct tacagcagat caggtaaatg gtgaagattg
      541 gatcggcggc ggtgtcggcg ctaactctga gtctacaggt tggcttggtg ttgaatgggg
      601 tcaagaaggt ggcaagaagc ccatcatttt agccgaagac ggcacagtta catggcttca
      661 ggataagccc gtgtttgcag atacagacac atattgccag ttatgggcac agacatggag
      721 cattgacctt acacttgaaa agatggaagt tcttgacaag gacggcaagg ctatagaagc
      781 tatctctgat gttgaggctg atgagcccgc tgacacagat acaaagcccg ctgagcctaa
      841 gcctgttcag acaaaggcag aaatcgctgc tgttaactct aaggaacctt cttacaccta
      901 cacagttaag ggtgacattg acactcttaa ggttgatgta gttgtaagcc ctaatgaaga
      961 ggacaagtca tttgaatgga atgactggtg cggcgcaggc gttatcgtta agaaccccga
     1021 tggtacaact aagtactatc agtggggcgg tgctcaggta agctggggct gggatgcaac
     1081 tggtgataaa gagcccgatt ctatcgacgg cgttaaaggc gaaacatggg taggtacagt
     1141 aagcgcagaa gctagaagcg gtcagctttc tatccccgtt gttaagggct ctgtagttga
     1201 gttctactgc ttaagctggg ttgatgacgc aacaggcgaa gctaagtatg acaagacaca
     1261 gtacacatta acaatcaacg atgaagaagt tgcagctgac gacaacaaga ctcccgacga
     1321 cgatacaagc aaggaagatc ctgctaagcc cggcgattct accgttcccg gcgattctac
     1381 cgttcccggc gattctactg ttcccggcga aacagaagta cccgttgagc ccggtaagtc
     1441 tgacaagaag cccgctgatg acaacaagaa gcctgcaaac cccgatgccg gtgttgcagt
     1501 agcagttgtt cctacagcac ttgcagcagc agcagttgca gcagcaggta tcgttcttaa
     1561 gaagagaagc aagtaatcat tcgtgattag gtaaagcttt gaattaaaga gcatattgac
     1621 ccctccgctt tggcggaggg gtttttatat atgcggatat atcgcgatac cgctgtcgga
     1681 tgtaataaga gccgcctgtt ataagcaggc ggctcagttc gtttatatgt atttattctc
     1741 cgtggaacgg tctacgcttg ttttcaagca cagtctttac tattgcggag tatatttctt
     1801 ttcgagcgta attgcagtaa ctgtcaataa cggatttttc ctcgtcaggg gttactgtaa
     1861 ctatctttac cggaacttgt tcaagcacct tttctgaaat tatgcgcttg catttttcgc
     1921 agtgacaggt gttgaaacgg cacaacgcat catcaaggat ctcatcaatt atgatgcggt
     1981 tgatatcaat cgttatctga cctgtatgac gtgttacagg tctgctcaca gccgtttcac
     2041 tgctatgtga cgcgttatcg gcggttgcct cagggatatg aagcatcggg ttatgcttgg
     2101 gtctatgcgg cggttttggc gctgtagcac ctctctttga ccacgtgtag gtgtacagct
     2161 tgtcctctat cgtgctttca gcctttgctt tcttctcctt ttcctgctcg gcaggcggca
     2221 gaaaaggatt gacaccggac ggctttgcat ttattaaatc cattacttgt tttgtcttgc
     2281 ttgatttata ttcttctgcc attaggcggc tctcctttca ctttactttc tttcaggacg
     2341 tcttgtgggg cggtcactgc tttttttaga tgtgtttttc atactcacgc cggtaacttc
     2401 gcatatcagc tttttgtact cagccgcggc ttttgatcgg ggtgcatatt ccatgatgga
     2461 tatgccgtgt gcgggtgctt cggcgagtga aacatttgtg cttatacggg agttgagaat
     2521 ccttgtgccg agctgttcgg ctatcattcc cgcaagctcc tctacttcct ttgtgagcac
     2581 aaggcgggga ttgtatttgt ttagcagtat ccctgttatt tcaaggtctt tattgtaata
     2641 cttctttacg gcgaaaatcg tttctttaag ctgtgctata ccctgtaagc tgagtatttc
     2701 cgctatcatc gggattataa gcttgtcagc cgccgtatac gcgtttatag tgaggataga
     2761 cagcgcagga ggggtatcta tcattatgta gtcataatcg tctgctaccg ccgtcagagc
     2821 ctctctgaga agatattctc tgccgactcc ggtaagctcc agctcgcaac ccgacagcag
     2881 gatatttgcg ggtattacat cacagttgtc agtgctttgt atcgcttctt tcagtgtgca
     2941 gttgcccttg aaaacatcgt acatagtata gctttcctcg gaatctgcgc ctaaactgaa
     3001 gctgagattt ccctgcgggt cgagatctat cgcaagtacg ctctttccgc tttcggtgag
     3061 ccctccgcag aatgcggaac aggtggtggt cttgcctacg ccgcctttct gatttgttat
     3121 agctataacg actgccataa agtgcgtcct ttctttgtat tatcgggtaa gatttttaaa
     3181 tttcaaatta tttacacata aaacagtcat acccgcctga aaagcgggta tgtattgttc
     3241 ttgtgatatg cttagagtgt gtgctggaga tcctctatct gcgaatcttc gggctgataa
     3301 atatggtagt ttgccttgcc gttcttcttt acgaagtaca tagcctcatc ggctgcggct
     3361 actatcttgt tgctgtcgtc accgtgctcg gggaagaatg caataccgat acttgccttg
     3421 acatttatcg atacattggc aagctctgaa tgataacctt cgtagagctc gtcgataagg
     3481 tcaagtgaca ggctttcgat ctcctcgatc tgttttgcat cggtgataca gagtacaaac
     3541 tcatcgccgc cgaaacgtcc cgcaaaacca ctgcccttta cacgctgttt tatgcaacct
     3601 gcaacatatt tgataacctc atcacctaca gagtgaccga aacggtcgtt gatgaacttg
     3661 aagtcgtcta catctatgaa aagcacggct accttagcgt gtgcgcttcg ttcgatctcg
     3721 ctcttgagct cacgctcaaa tgtgcttctg ttatagagct gtgacaggaa gtcgtagctt
     3781 gcgcgctcgg aaagctgaag ctcgagcttc ttttcgctgt ctatatcggt tacgacaccg
     3841 ataacacgga gcggttcgcc aagacggtcg gtaacgcaaa gagcacgtat cgagaaccac
     3901 agcagactgc cgtttttagt agtcatctga tattcgccgc tgtgacctgt cttgttcttg
     3961 agtagtgtgc ttatgtcgcg cttgtatacc tctgcatatt catcgctcat aagcttgtca
     4021 aggaacttac cgtttgcaag ctgtgcttct ttcggatcga tatcaaactt ttctttgaag
     4081 ttatcggaga tatacatagc ttccttgtgg aaatcccact cgaacagcat attgtccgaa
     4141 atatcggata ttgtcctgtg cagttcttcc gacatactta cgtcatcaag catattgttg
     4201 aacagctcag aaatatcctt aaattcgttg tgcgttttaa tttcaagacg tttttcacgg
     4261 tcgccttcct gtatctcacg cgctactgta accatctggt tcatcggatt aactatagac
     4321 tttgtgacga ggataccgag cagtgcgcag ataaccgcga gcactaccat agagcctatc
     4381 gatattgcga gcggtatcat tatctgcgac atcgacatat tgtcggggta tacgctgacc
     4441 catttccaac cgcctgttga tgagcctgtg aagtaattgt agcagccgag cgatgagccg
     4501 gtgttatagt caatgttgtt ttcgctggaa gtcggcttta cggtcgattt taagacatct
     4561 ctcatactgc tgtcggtaac gtcatcgatc ttctgtaccg ctgttccgtt gtagttcaga
     4621 acgcagctgt tgccgtccga aaccgcaaga tagcctttgc cttcaccgta tgtagagttt
     4681 gaaaggatct ccgaaatgcg gctgatgtca atcacttcta tcgcaaagcc gtcaatgttg
     4741 ttgagcccat ccttgtcgat gctcttggca acgtagaata cgtcggcatc gtagtcctcg
     4801 ttattcgtat agatgttcga gatctttatt gagccttctg tatagttttc aagctcttca
     4861 aagccgaaaa agctctcctt cataccctgt atctcgttgt attcgtatac gatattaccc
     4921 gcagggtcgg tgataactat gttcttaaaa ccgctgtttc ttgtttcgat gttttctgag
     4981 agcaatgaga gnnnnnnnnn natttgactt tgcatcatcg ccctgaccgc tctgcgcaag
     5041 cactcctgcg gaattgctga acgaaagata agcaacatcg gatatgcact cgttcagata
     5101 ttggttgatt atttcctgct gtgcagcacc tgccgctctt gcaaactggc tgaccgaatt
     5161 ggcggcaaat cccgtaatgc tgaaatatcc gacaatgccg acaatcagca tagggataac
     5221 tgcaaaagcg gtagtaagta tgcgcaaagt cgttttgatt ttcaaagact gcatattgta
     5281 taacctccga agctcttact taaaacatat cacgtgtttc gcgtgatttc tgcaacattt
     5341 caaattgcag tttatagata tatcttgaaa tggtttcttc ctgtgaatgt gaaacaccga
     5401 taaagcgaca gccgtagcct gtatcagcct cttgcgatgt gatctcctgt tctctcagca
     5461 cttctgccgt aagctcaaga cgcgttccgg acagcccgac tatcatcagc agcctgtcgc
     5521 cctttgcaaa atgtctgcgt gtatcggaac atatgaagaa tacgccgccg acattgatat
     5581 ccttgatata gatctctgcg ggaggctcaa gcggaacggg cttttcgctg ccctcctgag
     5641 tcctcagtgt tataaacgcc cgttcgtttg tctttatctt gaaatacttt ctgcgctctt
     5701 caagcagctc tgccttgtcg ggccgcagca ggacgttaag ctgtcggtcg gtcgataccg
     5761 aaatattggt tttgtatctg tatctgtcgc cgtcgcgcat acgggcaata acatagacat
     5821 attccgatgt atcaagctcc gggagtctgc gcccctttat catcagcata ttgtcgataa
     5881 tgttatcgtt cgtatcgtac attaaaggaa agtcgaagtt gttttccgta tagaacagct
     5941 ttttaccgtc aagcgaagtt atttcaaaag aagtgaaatc ttcttttttg tagctcaaaa
     6001 acacaactcc ccctttcaaa gacacacggg gaaattggca taaactgcca cacccaacac
     6061 gcctataata tcatttaaca ttaagtatac aataaaatag ccaataaatc aagttaaatc
     6121 ggcggttttt tcgtcgacat ttaaaaattc tgcgaattgt ttaaaaaatg caggtcgttt
     6181 ttatacattt taattaagaa aagttcgttg acttttgtaa ggcggctata atataataag
     6241 tataaacaga agtttgcccg agaggaggtg caggtacgca gaattttttt cacgcagata
     6301 caaaaggata tcggaaaatg tgtacgaaag caagcatagc gatcggtgcg gtgcttgcct
     6361 tgcctgcact tctcagtctt atgtcgctga tacttcagac aagaaactgg cgcaccgaag
     6421 gctttgttgc ggcaagactt gccataccgg tcgtttgcgt tgccgcgtgg cttcttctca
     6481 gtcttgtgat atttcttgtc tgtgaatcaa gagcgcggca tataacccgc aacacttatt
     6541 ttgaaataca gaaagacgcg gctgtctaca gtagatacgg cggtcggata ttcagcgata
     6601 tcggcggcat cgagcgtaag ctttgggtga taccatatga cggactgaaa ctttccatga
     6661 aaaacggcag actgatattt acggggaaga taaggtatta tgagggcggc agtgacagac
     6721 tcacttaccg cataaggcaa ggaaagcctg aatttgacaa ctggtggctg aatgacaacg
     6781 gttttacaga agtgacagct gtcactttac cgccgtgctt tccgaagcag aaaaagatat
     6841 tcggatattg ccgtatagcg tttcagagat atatgagaaa caacgagaag aagcaggtgg
     6901 tgcaaagtcg ccccgtacat cgtccgaaaa tgatctacag gcaaaggaaa agaggtacat
     6961 atagcgaact gccgacattc gacagaaagt ggtaagctgt acgttgttgt gcagtctgcc
     7021 gaaaaaacac ggctgttgca catttaacgc ctatactttt attaaataag taataagtac
     7081 aaaaatcaac agttttttcc ttttagcctt gcaaaaaggc taaaaatgtt gtataatata
     7141 caattgagtt gttggcatta aaacaaaaaa attgttaaga taacaccaaa gctcatatta
     7201 tttttatata gctgaaatta cttttctata tattgtaatt ccgttaaagg gaatatacaa
     7261 aatacagaat tcgttaaacg cattaaccgt caggaaagac ctcagtctga aaaggaggac
     7321 atatgg
//
LOCUS       DAWAOG010000026         7264 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1473_length_7264_cov_2.553891, whole genome shotgun sequence.
ACCESSION   DAWAOG010000026 DAWAOG010000000
VERSION     DAWAOG010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7264)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1473_length_7264_cov_2.553891"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3105..3114
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6977..6986
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1615 a         1956 c         1543 g         2130 t
ORIGIN      
        1 ccgatcagcg cttctttgct ttcaactccg aagatcgcag ggtcgtactg cccggcatca
       61 agcttgtgga atatggggta ttcacggtaa acattaccct ttgcggcggc agttattatc
      121 cgctttgcta tatcgctttc aaagaatttt gcgatatccg ccgggtctat gctctttttc
      181 tcggctacag taaaaagatc tgccgccgtg ttttcgatat aagcctgtac catctctgcc
      241 gacatcacct tatctaacgg tatatgctcc ataagcttat ggtatgcatc gccgcggcgc
      301 tttccgctga gtctgccggt ctgcttttcg gtaaggaatg acggcttgcc tatataaagt
      361 ccgtagcttt caccgccata cctttctctg agatttgccg caagctctgt agcggtaaac
      421 tttgacggca taaccgtgag cggctcatac tcatagtgcc ggctcagcac ctcttttaca
      481 gcggctttga acccggtatc aacatcgtcg ttatcatcgg ctctttgtgc ttcgttcgat
      541 gcggtcacct cagaaacggc gcattcggga acggcggtct tgtcatcgcc gtcactgctt
      601 tcgcacagcg gatagaactc atatttcaca tctccgtatt ttataattcc ccgttttctg
      661 tcgtctaccc tgtcgcattc gcaggacagt acttcaagaa gccacagcaa atagtttgca
      721 tcgttacact tagctccctt acgtcccgca acggctatgt caagcttatc atattcctta
      781 tcggctatct ttttcgtacc cgtgaagata agcttgtttt cggctcttgt tgccgcaaca
      841 tacagcaggc gcatctcctc gctgaacagt cccgccttta tttcaagcga tgccgcgtga
      901 tatgcgggcg atggtatctt gctcattgtc gatatgtcaa cgctctttgt gcttatcccg
      961 tattcttcgc tgataatgat atccccgtta taatcattgg gattgaactt acgcgcacag
     1021 tcgctcacaa aaaccacagg gaactgcaag ccctttgacg cgtgcaccga cataatcttt
     1081 actccgtgac tgcttgcaaa agctgccgct ttcagcgaac cgcccatatt ctctatattt
     1141 tcaataaagt cacttatcgt tccgccgcct ctgaagtcac agtactcacg ggcatatgat
     1201 ataagcagtt ctaggtttgc ctgttttgat accggatcat cgcccaccga cagaagctcc
     1261 ggagcagata tagtgtcata tataatgcgt ataagctcat ggggcgaaga tgccgccgct
     1321 atatccctga acccgtcaag atcgcttaaa aaaccgatgc actttgcaga cggtgcgata
     1381 ttttcggtaa gagaagccaa caccggatat gccgatgtat cgtagccgta tgccgcaagt
     1441 cttaaacagc tgtacagcgg ctttttcttc atacatccgg gattatctct gtcacatgaa
     1501 taacgctcca gctcttctgg gcaggctttc tccaaagcag gtatatcaaa gccgaacgtt
     1561 cctgtcctga gtcttgccat ctcttctgcg gtaaagcagt acagcggcga cataagcacc
     1621 tttgcaagac ttatatcatc gtacgggtcg tttatcacct taagcagtga aagcataatc
     1681 cttatttccg ggcactcggt aaacacgtct ttctgcggga acgtcacgcc cagcccctgt
     1741 gattttaccg cctgtgcata tatttctgcc ttgtttgcgg gtgaacgcat tattatggca
     1801 aagtcgtctt cggtgcaagg tcttttttct ttgccgacct tatatccgct gtctatcata
     1861 ccgcgtatcc ttgacgcaac atattcggct tcgctctcgg cggcgacact cgtatacggt
     1921 gtgataacgc agacctcggt catatcatcg ggatctgtta cagctgtacc gtccttatca
     1981 agtccgcaga caagtctgcc gttcttctca tagtcaacgc cgcctgtctt ttcggtcatc
     2041 atacggtcga acagtgtgtt gataccgtcg ataacgctcc tgccgcttcg gaaattttcg
     2101 ttcagcggca gtacgtcaaa ccccggcgat gcacatatct cggagaaaac ctcaggctgt
     2161 gcgccccgaa agccgtatat cgactgcttt atatcgccga caaaatacag tccgtcaagc
     2221 ttatccgaca aatgcctgaa tatctcatac tgcaacctgt tgctgtcctg aaattcgtct
     2281 acaatgataa gctgtaagcc tattcttttt ccgacctcgg gattgtcgga aaggataccg
     2341 tacagctctc tttccgcgtc agaaaaatcg gcacagttgc gactgcgttt ttcctctgcg
     2401 taccttctgc cgaactttat cacaagctct ttgagcacag taacagcggg caggctctta
     2461 ctcatatcat cgtcaaacga tgatatttta agacaggtct tgtatatgtt atcctcgaaa
     2521 atgctctttg ccgcttttct gtgtgctccg actaatttct cattacttct gtcgggcggt
     2581 gctttatcga actttgtctg cgacagtgca gttatctctt ctgtaaagca acagttattc
     2641 tgaataagct catttatagc cttgccatag acgttttcca acgtgtccgc atatgccgcg
     2701 tgcttatccg cattactgcc ctcatagcat tcaatagctt ttcgcattgc ggaaatttct
     2761 tcatcaaaac gcttagcata gtatctgacc ttttcgataa gcttcagctt tatgttgttt
     2821 tgtatcccgg catcatcact gcgcgacagc caaccctcgg ggtcggtgtg attgagcgat
     2881 tttgcgtgca cgaaaaggat aatgtttctg agatcggcat cgtttttctg tatatagttt
     2941 tcaagaatga tatctttatc cgcactgtcg gcattcagat aaaaatcctc cagcacatca
     3001 tcaagcacag cattcttgag tgcacccgcg cgggtttcat ccataattga aaagcccgcc
     3061 gcaagatttt tagcaaggtc gatattctcc cttataagta taaannnnnn nnnnctttac
     3121 gcagtctgtt tctctgacgg cgtataaata acacgtcatc ggcatttgaa gcgtcagccg
     3181 cccgctcacg cataagcttg tcaagccttg ccctgagctc tcccgccgcc ttgtcagtga
     3241 atgtgaccac cgctatcttt gacgggtcga ggggatctgt tctgtcgcac accattctta
     3301 caacgcgctg tgtcagcacg gcggtctttc cgcttcctgc gcctgcggat actatagcgg
     3361 ggtgtctgcc ctgctctata gccttgttct gcgcatctgt ataattcatc ttcaatcctt
     3421 ccttacttgc tttcttcact tagcactata tcttcgctga atgcggcagc atcttcggaa
     3481 ttgacatctg tggcagacga tattcccgca ccgcatatat ccttatattc gcagtacttg
     3541 caggaaatag ccacgccgtt ttccgacagc ggctttgcat cgacacagcc gctctttact
     3601 ttacccgcat tttcctttat gacctttgct attctgcttt cgaggttcgc aagctttgtt
     3661 ttttcgatag ttgttgcact gtaaaggcat tcatacttct gcttaccgtt ttggtcaatg
     3721 tactttccgt tgtaattatc acgctcaagc tcctgttgtg tgccctcaac ggcaaagcct
     3781 gtttccctgt ggctgtcgag ccacattcct cttttaagct cttccgacgg gtcttttata
     3841 tccttaagcg gagtttttcc cgcaggataa tacatagccg cagacggctg acagccgtta
     3901 tccttattgt tatcgctcag tgtaagcaga tacagcagca gctgaagatc cagtccgtag
     3961 tagatacggc tcagactcgc ttcctttttt cctgtcttat agtctattac ccttatctga
     4021 cggcttccgt catcaccgta tgccatgtcc accctgtcaa ccgttccgcc gatagtcacc
     4081 gtctgtgtgc ttccgtcgtc aagtgttata tcaaagctgt acggcgatgc gtcaaattca
     4141 tcaacagatg aagcctttga cagcgtaagc tcaaaatatg tcggtacaaa gcggctgttt
     4201 tcaagctcgg ctgtcatata cttcagcaga tagaatgccg ctgttgaaag cgccctgtac
     4261 agggtgttga actttgcgct catttcactt tcgccgagca gatatttttc gcggtactcc
     4321 gtcatagcct ctgctacaag ctgttttatc gtgtcttcac cggcatgagg atttccgtta
     4381 tccttggcgt ttgcatacag cctttcaaag ctgtatttca ttatataatg cattatactt
     4441 ccgtagttta cggcgctcat tgttatatca aacgggcttc tcagctccat accgtactgt
     4501 aagaagtacg agaacttaca gccgttcagc ttttcgatag ccgtaggcga aagtgacgag
     4561 tacgggaaaa caagctttgc cgtttcgcct atcctgtgta tatccgcacc gtccgctctt
     4621 ctctcagccg ccgcatttat ccttgaaata gcggtatttt ccctgccgca ctctgtcagc
     4681 gcctcggtca ccgctctttt ctgcgatgct gtaagcgcag taccgttaag cacatactgt
     4741 ctgtctgccg actttgcact ttccataaga tacagcggag gaagctcatc gatatttaca
     4801 atatccgttc tgataagctt tgctgtctca ccgacgtatt ctgacgcatt ttcaggctct
     4861 cccgcaaatg tcacgaacag cttttctccc gcaagcgtca gcgctttata gcagtcaagg
     4921 accgcaagcg aatattcggc atcctcgtct tcgtttatat caagcccgct ctgtgcaaga
     4981 aattcattgt catcagctgt aaatatgccg ttgtcatctg ttcttatccc cggaaatgcg
     5041 ccatctgccg cacccataac gaacacggcg cgtacatctc tgctcctggt tcttgcagga
     5101 tcgccgaaaa gtacgctgtc gagcaccttg ggcgggcgcg aaaggtttat ctgagccgag
     5161 caggtacgga taagcgccgc aagctcgccg atcgtcatag gtgtatcgtc gagcgtttcg
     5221 tacagcgatg tcagcatatc cgacacattg cgccacaggt tgttatattc ctcggtaagc
     5281 tcctttacat aacggagctg tcttgtgctg ttgtcctgac attttccttg tattgccgcg
     5341 cttatatccg ccttgtcaaa caaaaattcc gtaaaacgcc ttacaagctc cttaccgccg
     5401 acatttcgca cagatttttc aaactccgtc agcggagaaa ctattttatc ccggatattt
     5461 tcgactgctt tttcggcatc gtcggtaata tgcgggaacg gtctgtccca tcttctgttt
     5521 ttcagatccc aacggtttgc gtattcttcg actatgtgct gatccctcag cgtcagcggc
     5581 acagtcttgc cttctttttt atccgagggt atacgcgcaa atccgcttct gatatatctt
     5641 aatactttta ttcctgtagg ctcttccgcc gcacataaaa gacaatccag ataacgtaac
     5701 agcggctttt cggacaccgg ctcaggaaga tccgcaaata tcgggacttc atatctgagc
     5761 gcagccgaac gtaccgcgtc cctgtacttt gccggagcgg cgcatatgac cgcgatatcc
     5821 gaatatctgt acccctcgca ccttacaagc ttccttatat ttgcacatac aaagtcggct
     5881 tccccgctca tattgcggga atatgcaagc gtcactccgt gcggatctgc gggcttttcg
     5941 gctttgccgc cctgcaagaa gtcgcgcagt actctgagcg tatcgttctt gtatcttacg
     6001 gcactgtcgc cgagccttgt atattctgtc ttctgcggct ttccgcagtc catggcgtat
     6061 tgattttcaa acagcgcttt tgtcttgtca cactctgaga atatctccgc cccgctgtcg
     6121 tttgtgcaga cggcaacggt aaaatccact cccttgtccg ccatagcgcg cagaaattcc
     6181 ttctgcgccc cggagaagct gtcaaagccg tctatgtata ctcttgtgtt ttcggggaag
     6241 caatcattct ccgctatagt ccttgccgcg agcagaaggt tgtcctgcct gtcgtcgtaa
     6301 agctctgtca gcatcttgtc ataaaggctg tagaaaagcg ctatatcgtg catcttgtcg
     6361 gtaaaatgcg ccggcgcaga gcttacagtg ctgtcttccg ctataatatc atacagcttt
     6421 tgcgaggata tcccggcact tttcagcgtt tcaacgcccg acagacacac cgatacaaag
     6481 ccgggctttt ttgcggcact gccgtagaag tcgaacgact ccgcaagcga ctttaccgct
     6541 ctgctcatta tggcgagctt tgccgtgctg tcggcataga tctttcgcgg acagtacttt
     6601 ttcaatattt tttctgccaa tgacataaat cccgtaacca taatcccgga ggtcttctca
     6661 tcaagctcat cggcaactct gcgttcgctc tcaaacacaa actggtcggg caccagcaat
     6721 actttatttc ctttgtcggt tttgagaaga gagtataaag ccgttgtctt gccgcttccc
     6781 gcagcacctg aaattatatg tgccatattg cttcctccgt atcaataatg ctatattcat
     6841 ataataccac attccttgcc ccttttccag tacactcacc gatattttcc ctgatttccg
     6901 ctcattttca acaaattctt tacatcacgg aaaaaatgta gtataatgat acagtaagct
     6961 gtgtgtcaga acggaannnn nnnnnncgga atacaacacg cggcagtttt taatcacaca
     7021 cgggacagcg tctgccgtcc ctgcattttt gaaaggaaac atcttatgtc tgaattaatg
     7081 ttaggtaatc aggcggttgc gaggggtctt tatgaggcgg gagtaagcgt tgtatcaagc
     7141 taccccggca cacccagtac cgagataacc gaattttcgt caaaataccc cgaggacgaa
     7201 atgtattgtg aatgggcacc caacgagaag gtagcctgcg aggtcgcaat aggcgcaagt
     7261 atgg
//
LOCUS       DAWAOG010000027         7217 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1485_length_7217_cov_2.646607, whole genome shotgun sequence.
ACCESSION   DAWAOG010000027 DAWAOG010000000
VERSION     DAWAOG010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1485_length_7217_cov_2.646607"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4182..4191
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4852..4861
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6950..6959
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2152 a         1600 c         1791 g         1644 t
ORIGIN      
        1 ttgcacacac gtacttgtgc tatagcacaa aacccataaa aaccggttga ccttacagct
       61 tttcggtagt acaatacagg taaagaaaaa caagtaccgc aacggcgcag gacattaaaa
      121 gttctgcgcc gttttgttta caaaggaaag gatgacgtac aaatgtatga atgcagttta
      181 gacagaatga aggataagat atccactttc agcaaattcg gtgatgcagg tcacggcggt
      241 atcactcgtt actcactctc acccgaggct attatggcgc gtaatgagtt catcaagcgt
      301 atgaaggcta tcggcgcgga gatagagata gatgatgttg cgaatatcta cgcaacgctc
      361 cccggcacag accctgatgc aaagcgtatc gtaatggctt cccactgcga ctcggtaaag
      421 aacggcggta actatgacgg tatcctcggc gttatgtcgg caatggaggt acttgaaaca
      481 gtacacgaaa agaacattcc ccacaagcat ccccttaccg ctatgatatg gacaaatgaa
      541 gagggttcgc tgtatccgcc cgctatgatg tgctccggta tcgtatgcta tgattatctg
      601 cctgaggaca tcagatgcaa gttcaggtat gaggatatga tgaactccaa gagtatcctt
      661 gacaatacat caacattcgg tgaagcgctt gacaagtcgg gcttcaaggg cgacaagaag
      721 taccgcctgt cacccgacag atatatgtat atgtttgaaa cacacatcga gcaaggccct
      781 atccttgaag atgcaggaaa cgatataggc gttgttgact gcgtactcgg tatgttcaac
      841 tacagactga agttctacgg tcagacgacc cacgcaggca cattcccgat gcccaagcgc
      901 cgtgacgcgt tctttgccgc atcgcaggca ctgtgctatc tgcatgagga gatcgacaag
      961 ctcggctaca gcgaccttgt ttatacaaca ggtgaggtcg tatgccaccc ttgcgtacat
     1021 acctgcgtac ccgacttctt tgacttctcg tttgactcac gtcacgagga ccccaaggta
     1081 cttgaaaagg tactggctat cgtaaagtcg tgcgaggaaa aaacatgggc aggctgtacc
     1141 tgcaaggtgg aaagagcttg gaaccgtgat acggtctact gggataagaa gctggtaagc
     1201 tatgtaaagg catcagcaga ggaatgcggt atcaagcacc agtatatcca ctcgggcgca
     1261 ggtcatgacg cacagtttgc cgcatatatg ctcccgacaa ctatgatatt cgtacagtcc
     1321 aaggacggtc tgtcacactg tgagcctgaa tattcaagcc cggagcactg caccgagggc
     1381 gcaacggtta tgctgaatgc tgtcctcaag gcagataatg attgattata atgacaacca
     1441 cttctttttt tggtatattt cctgactgat tttgctttat ctgatttact ttcacccgct
     1501 tttacggcgg gtgatttttt gcgccgtttt ccggcataat agctcatatt caaaaataac
     1561 ataataagtc ttgatttttt gtttaaacta tgttacaata atacagtatc agaaatagaa
     1621 cccgtgatta tgggtgtaag ccctgtgcaa gggagtcctg tgaaccatgt caggcgggga
     1681 accgagcagc attaagcagt catgacctgt gccgcagcaa gtttacactc ataatcatgg
     1741 gttctgtttt tatgtattga ttttgtcaca ggagggtcag tttatgcatc ttgcacttta
     1801 tcgtaagtat cgctctgcca cctttgacga agttatttca caggaacata taaccaccac
     1861 gctgaaaaac cagataaaat ccgggacacc cgctcacgct tatcttttca caggctcgag
     1921 aggtacgggc aagaccactt gcgcaaagct gatggcaaag gcggtaaact gcctttctcc
     1981 cattgacggc aacccttgcg gcgagtgtga aagctgtaaa gcgatagccg ccggctgccc
     2041 cgatataatc gaaatggatg ccgcatcaaa caacggtgtt gacgatgtaa gggcactgcg
     2101 cgatgaggtt atgtacgctc ctaccgtgtg taagtataag gtatacataa tagacgaggt
     2161 gcatatgctc tctgcatcgg cgttcaacgc tctgctgaaa acgatagaag agccgccgcc
     2221 gcacgttata ttcatacttg ccacgaccga gatccataaa gtgcctgcga ctatcgcgtc
     2281 aagatgtcag cagttccgct tttcaaggat cgatgtcgaa gagagtacaa aaaggctctg
     2341 tgagatagcc caaaaagaaa atgtaaagct taccgaggat gccgcaaggc tcatttccag
     2401 actgtcggac ggcggtatga gagatgcggt ctcactgctc gaccagtgca taagtgtaag
     2461 caatgatata gatgagcaaa cggtccgcac taccgcaggc atcgcgggaa cagaccacct
     2521 gttcgcactt gcggaatgcg tccgtgagca tagcgccgca gaagcgctta agataattga
     2581 cgaactgcat gatcagtcaa aagatcttat gcttttgctt gatgagctac tgtcgcattt
     2641 cagaaacctg tgtatgctga ctgcgacaaa taacgatttc tcactgatac ccgcaggaag
     2701 cggagcaaga aatgaccttg cacgtcaggc gggggagttt acactcggtg agataatgcg
     2761 ttgcatggat attttgcagg actgcatcgc aaaaactcct aaaaccgcaa aacgcaagac
     2821 ggttgcggaa atgtgcctta taagactgtg tacgccgcgt cttgacagcg acacatcggc
     2881 attgtcgtta aggcttgaaa ggcttgaaaa cagactggat aagctgtgtg acggcgaagt
     2941 aaaaattcag ccgagaagcg ccgccccggc agacgcggga tacgcagagc ccgtaagacc
     3001 cgcagacaag cctgttatac cgacagaaag caagcctcgg gacattccga aggctgcatt
     3061 aaataacaca gaaaataaag caaatacagc ggatataccg cccgcttcaa cagagccgcc
     3121 ggatgataag cccgactggc tgttcgaggg aacaggcggt gacgcttctc ccgtaaatag
     3181 cggcgacaac tcggaagctc ctccgtttga ccttgacgag ccgacagagc agacgcaatt
     3241 tgcaccacag accgatgaca gcaagcccga ttggctgttt gagggaacag gcggtgacgc
     3301 ttctccagta aatagcggcg ataaatcgga agctccgccg tttgatcttg atgagccgac
     3361 agcggcagat acacccgttg ttccgcttaa gccggagcaa gagcctgtaa cagaacagcg
     3421 accctctgcg gcaaagccgc aaggtgcagg tgttgccgac ccgcaggtag cagagatact
     3481 ggacagactt cccgttatac ttcaggctat actggcacag gttcgggtat cactcggtga
     3541 aagcaccgta aatatcagcg gatatcagaa gttccagtac gattttctga caacgggcga
     3601 ctcaaaggag cgccttgaaa aagccgccga agaagtgctc ggcagaaaag taaaaatggt
     3661 gtttgacggc gatgacggcg cagacacgca gacagctgaa agcgaccctg tttccgactt
     3721 ccttacaaag gcggaaagta tgggcgtaaa aataaaatac aaaaaatcaa aaaattaagt
     3781 cggctgactt aaagatacga aaggaaaaac cacaatgaaa gcaagattac ccaaggaata
     3841 tcagaacaga ggcgcaaaca atatgaacag catgataaag caggctcaga agatgcagga
     3901 agacattgag cgtgtccagg ctgaactcga ggcaagagat ttcacggctt ctgccgccgg
     3961 cggtatgatc gaagttacta tgagcggcgc aaaggtactc aaggaagtaa agcttaaccc
     4021 cgaggtcgtt gacaaggacg ctatcgagga tctccaggac gttatcgtgg caggcgtaaa
     4081 cgccgttatc acgcagatcg aagaagtaag cgcagcagag atggaaaagg taacaggcgg
     4141 agttaatatt ccgggtcttg tttaatggct gaatataatt cnnnnnnnnn naaagcttcc
     4201 cggcataggg ctaaagaccg cacagcgtct tgcgtattat atactaaacg ctcccgagga
     4261 agacgttgag cagtttgccg caggtatact ctcggcaagg cgcagtatga agatttgtaa
     4321 ggaatgccag aacatatgca gctccgacaa gtgcgatata tgctcaagcg accgcaggga
     4381 tcatagagtg atatgcgttg tcgaaagccc caaggacgtt tccgcaatag agcgtagcgg
     4441 cggctttgac ggagtatatc atgtactgca cgggctttta tcgccgatga acggcatagg
     4501 accagagcaa ttgaagataa aagagcttat ggcaagactt tctgatgcag atgaggttat
     4561 tatggctacc agtccgacta ttgagggaga agccacagcg acttatcttt cgcgaatgat
     4621 aaagcagctc ggagtaaagg taacaaggct cgcttacgga ctgcccgcgg gcgcttcgct
     4681 cgaatatgcc gatgacgtaa cgcttcagag agcactggaa aaccgtaacg agatctaagg
     4741 cagcatcgca tgattattat agcgcggttg tcttaaaatt cagaattact ttcggggaaa
     4801 ggaacacgat aatgacacag gaacagttaa acgcaattca ggcaaaaacc annnnnnnnn
     4861 naccaaaaag atcacaaacc atcagttact tgagatagta aaaagattga aaaaggcatc
     4921 ggatgaaaag gtaaatcccg agcctgagga gcaggcaaag tatatcgacg cactgatgga
     4981 agcaaagctt attgcaccgg tcactataac cgatatgaac ggcgaaacgg aaaatcagat
     5041 aaaggtgcag ttctccagcc ttacaaaccc tcagaaggaa agatacttta tggtattcac
     5101 cgacattgaa accatgcgca agaacataca ggataccgac aagatctttc tgatcgccgt
     5161 tacatacaag gatctcgcgg ctatgctcgc ttctcccaac tgtgctatga agggttttgt
     5221 tatcaatccc ttcacagaga atataatctg cggacctcag cagtcggagg ttatagcccg
     5281 ttatatcaat cagaagagat ttaacaacgg cgagcttact gtcatcaacg aagtaacagg
     5341 cgtacccgaa accgtaacaa agcctataat aaagtacttt gatgagcgca aggatgttaa
     5401 aaaggcatac ataatgaata tgcgcaaggt aaatcagctc aaccgtctta taattgttga
     5461 ttatgagggt gaggacgata agttcaacga tttcgcaagc gatttcacag acaaggtgct
     5521 caaggacata aatgatgaaa aagcaccgtt tatggtaatg aacttcaacc agcccgccgc
     5581 aaagcaggca accaaggaaa aagtaccgtt ctatgtaaaa gtgggataat gtacggctat
     5641 tgacccgaca agaaatgggt ttcacagaaa aatcaacaca aaaaaaagag gcgcaagcct
     5701 cttttttgtt tattctgcgg aatttttcgt taattttttg catttacttg acgaaaaagc
     5761 ggtaatgata tatcattata ctgtataaat gggttcagat acagaacagg agacagtatg
     5821 gatactatta tttcagcatc acttctggcg agtgatcttg caaatataga aaaagaaata
     5881 cgccgtacag aaaatgcagg agtagactgg cttcatattg acgttatgga cggagtattt
     5941 gtagataata taacttacgg aaacaatgtt gtgaccgcta tgcgcaaggt aagcgatata
     6001 tattttgaca cacatctgat ggtagcagac ccgacaaagc tgataccgtt gtttgcaaaa
     6061 gcagggagca atatggtgac catacacctt gaaagcaaag gtgatacaga agctaatctt
     6121 acggagataa gagaagcggg tatgcagtcg ggacttgcta taaagcccgc cacagactgg
     6181 aaagaggtta taccttatct tccgctttgt gatatggtgc ttgtaatgac tgtcgaaccg
     6241 ggttacggcg gacagggctt tattcccgaa tgtgccgata aaatcgcgaa gcttcgcaga
     6301 tactgcgaag agaacggctt cggaaaaatg aacattcagg tagacggcgg tataaacgcc
     6361 gctacagccg taacggtaaa aagcgccggt gcaaatgtgc tggtagcggg aacttatctt
     6421 ttcaaggcag atgatatgaa gaatgccgca gacgcaatac gttaaaagca ggacggcagg
     6481 atatcgagcg ctttatcggt aagcaatgaa taatactctt cagtgtcgca cacgatcagc
     6541 tcgtctgtat atgtcagcac ggaatattcc gcagatatgc ggagcacagc cgttatatcc
     6601 gatgtcgggt atacgaggag tcgatagctg ttgtctgtag tgcagtagta aagaagcgct
     6661 ttatgcgatg atcttttcaa tgctttgcac aaagcggcaa gctcgtttac cgatcctgta
     6721 catactattt cgttgttgcg cgatgatctg tcggtatttt tctgcgtccc cttaaggcaa
     6781 gaaatataca ggatacaccc gccgttgctt ttcgggaacg cttcgaccag caaacgttct
     6841 ccgcacagct ttaagccgtg gtcttcgctc aggcaagata taagtcttga cagcgcaagc
     6901 tccgcagaat gcgaatgccc ggatatatcc tcgctcttta tgtgatagcn nnnnnnnnna
     6961 gacacctccg ataaacataa ttacagacag ttttacggtc tgaaaggata aaaaaagtaa
     7021 tgaacacacc tacaaatgaa gctttgctta aaaagataga tgaagcgcct ttgcttatgt
     7081 caagggaggc ggcgcgaaaa aagctccgta agccgcgcag tatatacggg gatcagatac
     7141 tgtttatgct tgcgataacg gtaatagcga cctgctttta cggttggcgc gtagttgccg
     7201 tatgcgtatg ctcggtg
//
LOCUS       DAWAOG010000028         7141 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1496_length_7141_cov_2.212250, whole genome shotgun sequence.
ACCESSION   DAWAOG010000028 DAWAOG010000000
VERSION     DAWAOG010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1496_length_7141_cov_2.212250"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2692..2701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1838 a         1637 c         1649 g         2007 t
ORIGIN      
        1 ctgtacgagg agctcggcat aagccacgat ttttcacacg accgccctat gctgactgta
       61 cattttgagc gcgggtttga cgatgtttat gtaatacaca aggatatagc tgtgtccgag
      121 ctaaaattac aggctgagga agtgcaggcg gcaaagtggg cggacagaga tgaaatatat
      181 tcgcttatcg acagcggcgc ttttataccg tactacaaaa gctatattga tatgctgttc
      241 aactacagaa caaaaagagg taacttttca aaggagtaaa atatgaatga cgtatgccga
      301 atgataaaaa aacaaacgat aacgaacttt atcaatatgg aaacggcaat taaaacatac
      361 gacagaaacg caccggtctg cggttcgccc gcatggcgat atgtatatca cgccattcac
      421 tcggcggata agtggttttt taatcccgca aaatttacag aacccggttt tcacgaaaac
      481 ggaatggaca accccgataa tccgtgcaac aaggttttaa gcaatgagca gttgctcgat
      541 tatctgcata aagtgcgggc taaaacggaa aaatatcttg atgagctgag tgatgaagag
      601 cttgacgaaa agcccgacgg ttgtgaatat acaaggcttg agcttatcct gggacagttt
      661 cggcacattt cctcgcatat aggaatgata aacggtcaga ctatagagag aacgggaaaa
      721 ttcccgatat ttgcaggact tgacggaaag cagacagaca aattatttga cgaataagaa
      781 cgaaggaaca gaagatgtac attgattttc atatacacgc atacgccgat gaaatagccg
      841 acagggcggt acaaaagctg aaagatacag caagctgtga agcttacacc aatggcagaa
      901 cagacgacac aaggcagaag cttaaagagt ggggagtaga ttacggcgtt ttactgccgg
      961 ttgcgacaaa gcctacacag cagacgacta taaacaactg ggcgatagct cagaaccacg
     1021 gaaatataat atcgttcggc acagtgcacc ccgacagcga ggagcttgac agtgagcttg
     1081 aacgtatagc cgtgtccgga ctgcacggtg tcaagctgca cccggcgtat cagggacatt
     1141 ttatgtttga gccgtgtatg cagagaatat ataagaaatg cggcgagctc ggactgcctg
     1201 ttatacttca tatgggatat gacccgatat cgacaatgat atcttactca atgccgtgtg
     1261 accttgccga gatggctgaa aagtatcccg actgtacatt catcggcgct catatgggcg
     1321 gaatgatgaa ttgggagagg gttcttcact atataaagga cacaagtaac atttattttg
     1381 atacggcgta ctgcgcaagc tttatatctg atgaaatgct tatggaaatg ttcagggcgt
     1441 acggtgagga caggatatta ttcggcagtg acttaccgtg gagcgatccg cgcgatgaga
     1501 taaatatgat agacagaatg ccggtaagcg acagcgcaaa ggataagata ttctataaga
     1561 atgctgttga gctgttgaaa cttgatgtat aaaatagagg gcgtgactta aacagtcacg
     1621 cccttggttt ataattcgtt tattcggcat ctttacctgc cgccgcaagc gcgatagcgc
     1681 attctatacg ctttttctga agctcgcagg acagcttgtg gggcttcttt acatcaccct
     1741 catagataaa gctgttggcg ttgcaaccgc ccgagcagta aaatctcgcc cagcagttac
     1801 cgcagttttc cttgctgtag atatgtatac ccgcaaagta atccttaatt ttctgatcaa
     1861 ccttgcctgt gaagatatcg cccatcttcc attcctcaat tcctacgaac tggtggcatg
     1921 ggtagatatc gccgtcgggg gtaacagcaa catactcgtt accgcagccg caaccgcgca
     1981 gacgttttac agcgcaagga ccctgattta agtctatcat aaagtggaag aagttgaacc
     2041 tgcgcttgtt cgcaagcttc tgctcttcca tcagcttttc aagtctgtca tattcggcaa
     2101 atatcgtggg caggtcgctc tctgtaatag cgtaaggcat ctcgggatcg gtcacaacag
     2161 gctctaccga gagctgctca aagccgaggt cgcttatatg caaaacatcg tttgcaaagt
     2221 cgaggttata ctttgtgaac gtgcctctta cataatagtc ctttgtacct ctgcctgcta
     2281 ccagcttctg atatttcgga acgattatat catagcttcc cttgccgttc ggagtgggac
     2341 ggatattgtc atttacttct tttctgccgt caagcgataa tacacagttt gacatttcct
     2401 tattgatata gtcgatcttt tcatcatcaa ggagcatacc gtttgttgtt atagtgaaac
     2461 ggaagttctt gccgtgctgt ttttctatgc ttcttgcgta ctttacggtt tctactactg
     2521 tttcccatgc cattaaaggc tcaccgccga agaagtcgac ctcaaggttc tctctgttgg
     2581 cgctgtgctc aataaggaag tccatagcct tcttaccagt ttcgggcttc atcagcattc
     2641 tgtcgccgcc aaagtcgcct gtctgggcaa agcaatattt gcatctcagg tnnnnnnnnn
     2701 ngccttgata ggcgctttca ttgccattgc ggcgtactgc tcgtaatcgt cctcggtgaa
     2761 gagcatcttt gcgtcataca gctccttaag ctctgcgtag cattccttta catcttcctt
     2821 gtcgaagccg tccatctttg caagaacgtc atcgggacat acctcggcaa aaggcggagc
     2881 aacatagtca aggagcttat atgtcagctc gtcaacaacg tgaacgccgc cgctgcaaac
     2941 gtcaagtaca atgtactgac cgttctgctc atacttatgt atcatttctt tcattggtaa
     3001 attccttttc tgagttatat aaggcggtta tccgccgaaa tattcaaaca agccaaaagc
     3061 tcccgtattt gttcgggagc tttcaaagct tttctcttac ccgcggctta cttgtttaaa
     3121 gcctcgcact tctggttagc tactgtgcag cttgtcttac aagcggactg gcatgaagtc
     3181 tggcatctgc cgcaaccgcc cttagcggct gtttccttga ggttagcctt gttaacagtc
     3241 ttaatgtgct tcataaaaac acctccgttt atgttatcat tctaattgta tcataaagat
     3301 acggaaattt caatagttat tttgaaaaat tacatccagc cgcagtcgtc atactcgctc
     3361 catttgtcct caagctgttg ctgctgtgac gcaaatttca taacgatata ggcaacgcac
     3421 gctacaacag ctatgagagc gcctataacg ggaacaacgt aattcttttt cttcttttct
     3481 tccatagtat ttttcctttc ggtattatta aagcctgacg gcagttttac atattaagat
     3541 attctttata tatctccgac tttgagaacg gagtttttcc tgcgacctgt ttgcaggcat
     3601 cggacggttt tatcccgctg tcaacaagct ctttagccat aacggcggca tcggcaagag
     3661 aaatatcctc attttctttc tgcttttgtt tgccctcgac tacaagtaca tattcacctc
     3721 tcggcgtaag ctccttgtaa cgctctatca tatcggagat cgtacccttg attacttcct
     3781 cgtgtatctt ggtaagctct ctgcaaaggg ctatctgcct gtcaccgaga actctgtaca
     3841 gatcatcaag cgtgttttta agcttatgag gcgcttcata gaatataagc gtccttgaaa
     3901 agtccttgat ctcgtcaagg tgctcatcgc gttgcttttt ggtaacgctt aaaaagccct
     3961 cgaagctgaa gcgtgacgta tcaagcccgc tcatacaaag cgctgtgatc gcggcactgg
     4021 gtcccggtac actttcaatt tcaactccgt gttcgtgaca ctctcttaca agctcttcgc
     4081 ccgggtctga tatgcaaggc atacccgcat cggtcactat agcacaggtt tcaccgtcaa
     4141 gcaggcgggc ggttatatct tcgccgcgct tgtttccgct gaacttgtgg tagctcacca
     4201 taggcttgct tatagagaag tgggtaagca gtttctgagt tactcttgta tcctctgcgg
     4261 cgataaaatc gaccttgccg agtgtttcaa cagctctcgg cgacaggtcg gacagattgc
     4321 ctatcggagt gccgactata tatagttttc ctgccatgta ttgtgtcagc ctttctgcat
     4381 ttttgtgccg taataaattc tgttcatttc gttgctgtag ccgttttcac tgtagacctc
     4441 aaagtccgga agtatctcca tgcccggctt tccgcctttt ttaccgctta tcagtacaag
     4501 ccacggcttg ccgcccctgc ggttcaccac gttttgcata accttaggtt cgatcccgtg
     4561 tgaacgcata gagcagatca catctgcaag cctgagcggt atctggcata tcttaagact
     4621 tccgccatat tttaagagtg aagccgcggt tttcgtaaca tcgtcgatat tgcacagtat
     4681 ctcgtgcctt gccgccgcct gcgcctctga aaggcgttct tcacccgaac cctttttcca
     4741 gtacggagga tttacggtca ctatatcgaa atattcacgc ggaacatctt cagggtcttt
     4801 gaggtcgcac agtacaggct tcaccctgtc cgaaagatcg ttttccgcaa cgctttttct
     4861 gaacagttct accgcttccg gcattatctc aataccgtat atctctttcg ggggcttttt
     4921 ggatatgttt ctgcacatta taagcggaac tatgccgcag cctgtgcaaa ggtcgcacac
     4981 cctatggtgc ggtgcggggt cggcaaaatc cgccagcaga aatgcgtctg tgccgaagcg
     5041 gtggtcatct gaaacatata tctttgtacc ggaaaggtca aagctctcgt aattctccgc
     5101 catatcagcc taccagtata tttccctcgg gcaagatcct gttcttgtcc gagctgtgat
     5161 tttttcttat aacaagcgag gtcataagcg ttacaggacc tattcgacct gcgagcattg
     5221 tgaatatcat tatcagcttt gcgggaatat tcatctctgc agacgcacct acagagaagc
     5281 ctgtagtaga gaatgcggaa actacctcga atattgtttt ctgaagcgac ataccctcac
     5341 agcacataag tatgccggta aatgaaacgg ctattaaagt cagcgacaga acgataacgg
     5401 tgaatgtttt atatatggcg tcacgcttta tcctgtgacc caaaagatag gtatcctctc
     5461 tgcccttcag cacggaaatt accgtacaaa cgacaagtgc aacggtcgtt atctttatac
     5521 cgccggctgt ggatgcagga gcggcaccga tgaacataag gatggtgata attatttttg
     5581 taaagggcat aagatcgttc atatcaaagc aggggaagcc tgcggtacgg gagcttgcgc
     5641 ttaagaatat agatgtgagg attttttctc ctacgggcat ctgtccgagc gtgtcctgat
     5701 tgctgaattc tactataaat attatagcgg cgccgacaag cagcataact gcggccatta
     5761 taagaaccag ctttgtatgg agaagtagac gttttgtttt gttataatgt cttatgtttc
     5821 gccatacgat aaagcccaga cctccggtcg ttataagcac aatgagagtt atcataacta
     5881 aagggttatt tgtatattcg ctgagtccca cagctccggg tgttgctgtg agcaagtcaa
     5941 atcctgcgtt gcagaaagcg gaaacggaca ggaatatcgc ttcaaaaact ccgagtgtgc
     6001 cgaagtcggg aacaaagaca aacgaaagta tgacagcgcc gacaaattcg atggcaaacg
     6061 agtaaaggac aatttcatag aaaagtcttt tcggtgtggt ttcaagggca ttgtcggtaa
     6121 ggtcgcttgc ggctatcttt acggtcgacc agcccagctt tttgccgagt ataaggttga
     6181 tgaacgttac caaggtcaga aagccgagac cgccgacctg tataagagcg agtatgacac
     6241 cctgaccgaa gccgttgaaa taattgaagg tatccaaaac ggttattccg gtaacgcagg
     6301 ttgcgcttgt ggcggtgaac aggcagtcta tgaagctgct gaactgaccg cttcttgaag
     6361 atatgggcag gaaaagaagt gttgcacctg tcagtattat gatggaaaag cccagtacga
     6421 gcatccttga cggttgcatt tgtcgggttt tctttttgag tttaggcatt tttcgagttt
     6481 ctctccttgg tttctttgtg ttttattatt tgctttcgga aatgttgttg tcttcgggtc
     6541 tttcaggcag atcgtcttta tggtgtctga ctgtattcct accgatggca tatacattta
     6601 tgtcgtttat acgtccgata agtacgcacg cgccgtcagt ccttaccgaa tcggtgctga
     6661 taagatagca gtcggaagga atagcctgac cttcctttat atattttacc gttgaggact
     6721 gattatagta ttgtatgctc attgcggtca tacggtcttc atcataaacg gctatagttt
     6781 tattttccgt gccatccgaa taagcggtga tacattcgtt gattttgagc gtgtcggata
     6841 cattttcact gctttcctcc gcatagccga acagcccgct tgaggcggtc gctatgcttg
     6901 catatattat tgctcccgtg aaaatgaaag cggttatttt tgatgcgtgc atcttggtgc
     6961 aacacacgac aggtactgcc gccgcaaata cggtgaacag caggcagaca gggtacagca
     7021 tatccgagct tgtaagtccc gatgatgcgt cacagccgat aagcatagcc gtgataccgg
     7081 gtgtaataac gttgctgagt atctgagtgc ctgctttcga ggcgttgtaa agaagcagag
     7141 c
//
LOCUS       DAWAOG010000029         7121 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1499_length_7121_cov_1.866686, whole genome shotgun sequence.
ACCESSION   DAWAOG010000029 DAWAOG010000000
VERSION     DAWAOG010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1499_length_7121_cov_1.866686"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1701..1710
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4134..4143
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5061..5070
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5804..5813
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1877 a         1640 c         1500 g         2064 t
ORIGIN      
        1 gctgtatatc gtttgtgctt cgtgtgatga ggcttgccgt tgagaacttg tctatctcgg
       61 tgcttgagaa gccgactaca ttcttgaaca cccttgctcg taagtccctg ccgacctttg
      121 ccgctgtagt tgccgcaaaa aagcctacaa ttacagacac agccatcata agaaatgcta
      181 taagaagcat ttttccgcct gttgtaagca gatagctctg ctgtagggcg ttcatatcaa
      241 cgccgagaga tgaatattcg ttctttataa aaagcacagc cgattgttcg gtaatagttc
      301 ccggcatatc cgataatttg tcaagcgaag cggatagatc ggtattgtcc ataacgcctg
      361 tgccctcaag cgcgataaga acagtaagag cgttcacctt atccacatca atgccgagcg
      421 cttcgcctgc cagctcgtat gatgtaaacg gcaatgtaat accctgcttt tccaccatag
      481 ccgaaaactc ggcaaacgac aattcactca gcatttctgc cgctgtcata ggtgatttca
      541 ctgcatcgat aagagcttgc ttttcctcgt tatcaagatc attcagaaaa aacacatcgt
      601 tgctttttga agcgggatac atatttttct gttcatctgt accgttgcct gcgcttacgg
      661 gtttatagct tttttccagg acacttttct gttcatcgtt cataaatact ttcagcgcat
      721 tatagccgtc aactgtaagt gcacctgccg ccgcgtcttc gataccgccg ttttgtatac
      781 cgatatctac tatatccgat gtatactgcg gcaaggtaag atcgccccat gcctgaatta
      841 taagcaggac gataataagt aaaacctgtg gtatactgtt tttcagatac ccgaatattt
      901 taaacaaacc ttatgcttcc cttcactttg tcttgagttc acctgatttt tcataagcat
      961 cggcaaaacg caatatcagt tcaataagct tatccgtatc ttcttcgccg agcatctttg
     1021 caatattgtc aagtcgtgtg aatatctcgt catatttggc ttttacagta gctctaccgt
     1081 tttcggtaag cttaatatat gttacccttc tgtcatcctt taatacaaca cgttcgacat
     1141 agcctttatc ctcaagctta tttattgtct gagagagatt gggtctggtg aaattaagat
     1201 agcaattgag ctcgctgagt ccgaaaagct ctgtaccggg cttttccgcc actatctgcg
     1261 tcattgccca gaacacaccg gtttcgcttg cgttcagacc ctccattatt ctgtactgcc
     1321 ttggctgagc cgaaaacttg ccaagcgcaa ttataagtct cattattctg tcatccatct
     1381 taatatcctc ctgtgacaag cagattttcc atacgatctg catttaatca aacctcttgc
     1441 ttaacgaatg ataatatggt atacttttta tgttaacgga ttataaattt aattaacttc
     1501 tttgaacgga gcagaaatga aaacaattga taccgatata gcaattatcg gcggcggctc
     1561 atcaggactt gttgcaggta tcgccgcctc tcgttacggc tgtaataata tcaccatact
     1621 cgaaaaactg tccagagtcg gcaagaaaat attatctacc ggtaacggaa gatgcaacct
     1681 atcaaatact gcggtttcgg nnnnnnnnnn tgagcggtga tatagaaagc tcggaagcat
     1741 ttttctcttc tttgggtctt atctgcaaaa cagatgatac gggcagagtg tatccttaca
     1801 gcaatgcggc ttcatcggtg cttgacgcac tgcgttttgc caccgatttt gcgggtgtca
     1861 atacgatatg cgactgtcgt gtgaccgata taataaagac aaagcgcggc tttattctga
     1921 cagctggcga tacaaagata agcgcaaaac gtgtgatact tgccgcaggc ggaaaagcct
     1981 caccgtcact cggtacagac ggtgacggat acgagctgtg caaaaagctc ggtcattcga
     2041 taacgaagct ctaccctgcg ttagcaccga taagaaccga tgtcggactt gtaaactcag
     2101 taaagggtct gcgtattgcc gccgacgctt cactgattat agacggtaaa accatcggca
     2161 ggcagtcggg ggaagtgcag tttaccgatg gggcactgtc aggtatctgc atattcaatc
     2221 tgtcggcaga agccgcaaaa tatgtcaata aaggcgagat atcgcttgat acagcaccga
     2281 cactcggcaa agaagagctt tacggcataa taaagaacgc ggcggaaata agagcaactc
     2341 ttccctgtga ggagcttctt acaggtattt atcacaagcg tataggtcag gctctgctga
     2401 aaaaagtagg aatacccttt tcaaagacct gcggcgaaat aacgccaaag gaaatatctc
     2461 tgctgtgctc agccgcaaag gacagccgtt atcctataaa aggcgtatca gattggagtc
     2521 ttgcgcagat tacttgcggc ggcattccgg ccggcgaggt ttctgacacg cttgaatcga
     2581 tcaaagcaag tggattgtat ctatgtggtg agatactcaa tattcaggga aaatgcggag
     2641 gctttaatct tgattgggcg tggaaaagcg ggtataccgc aggaatgaac gcaagtcttt
     2701 ctttatcgga gtaagtgatg ataaaataca gcaatataaa aatgccgctt gaacacacaa
     2761 aagatgatat agcggcattt ataagaaaaa aattaaagct tgatacgctt aaatcggcag
     2821 acattaaaat aatcagacgt tctgtcgatg cccgtcacaa ggatgatata cattttgtct
     2881 acacagttgc tttcccatat gaaaacgaaa acggtatact aagaaagaac acaggaaata
     2941 aaagcgtttc cttatataaa gaaatactgt attcttttcc cggaaaagca aggctgtcaa
     3001 aacgtcccgt gattatcggc ttcggacctg cgggaatgtt tgcggcgctg tatcttgctc
     3061 agtgcggtgt acggccgata gtgcttgaac gcgggcttga cgtagacagc cgcaaggaaa
     3121 aagttcgctt attctgggag aaaggtatac ttgatacaga gtgcaacgta cagttcggtg
     3181 agggcggcgc aggtactttt tctgacggta agcttaacac aggcgtaaat gacccgctgt
     3241 caaggatagt gtttgaagag cttgtgcgtc acggtgcacc cgaagaaatc atatatgacg
     3301 caaaaccgca tataggaacg gataagctgt ctgaaacggt gaagagtatc agaaacgata
     3361 ttatatcact cggcggcgag gttatattcg gcgctaaatt cacaggatac gatgccgaaa
     3421 acggcttgct caggtcagtg acctatgaaa aagacggtac tgaatgcact ttagaaaccg
     3481 ataaagctat tctcgccatt ggacacagtg cgagagatgt gttctatatg ctgaaaaaac
     3541 gcaacgtgac tatgcaggca aaaaatttct cggtcggtgt caggattgag cacagacagt
     3601 gcgaccttga ccgcagtatg tacggcgata tgagcggaca ccctgcactt cctgccgcag
     3661 attataagct ttctgtgcat gatgaaacag gcagaggcat ttatacgttc tgtatgtgcc
     3721 caggcggcgt ggttacggct tcatcttctg aagcagacac tatcgtcacc aacggtatga
     3781 gctattatgc cagaaacggc gaaaacgcga acagcgccgt tcttgtagga attacacccg
     3841 atgattttga gaaaaacgat ataatgggtg ggatagagct gcaacagaaa atagagaaag
     3901 ccgcctacaa agcggccggc ggtaattata aagctcctgt cgccttagtc ggtgactttc
     3961 tcgataaacg cgtttccgat aaattcggca atgtaaatcc gacatatcct atcggtacag
     4021 cctttgtgct ccccgatgaa tatctgccgg attttgtctg caatgcgctg aggtatgcac
     4081 ttccgcgatt tgcaaagaaa atcagctgtt ttggcttacc tgatgccgtt atgnnnnnnn
     4141 nnntattaag ggatagcaca ctttcatctg tcagcgtgaa aggcttatac ccttgcggcg
     4201 agggtgcagg ttatgcaggc ggtattgtta ccgccgcaat ggacggactg aggtgtgcta
     4261 tggcgatact cggcagagat tttgcaaagc gctaactgca caggctttcc atatcctttt
     4321 taagccgttt tataatacgc ttttcaaggc gagaaatata actctgggag atcccgatca
     4381 tatcggcgac ctccttttgt gttttttcgg gacggttgtt cagcccgaaa cgcagttcca
     4441 ttatcagctt ttccctttcg tcaagctttt ccaccgcttc aagcagtaca cgtttttccg
     4501 cttcattttc aatattgacg cttacggtat catcatctgt gccgagaata tccgataaaa
     4561 gcaattcgtt accgtcccag tctacattta gcggttcgtc tatcgatacc tcaaatttct
     4621 gctgatttgt ttttctgaga tacataagta tttcattttc aatacacctt gacgcataag
     4681 ttgccagttt gatgttcttc tcgacgctga atgtgtttac tgccttgata agtccgatag
     4741 ttccgatcga tatcagatct tctatgccga tgccggtgct ctcaaatttc tttgctatgt
     4801 aaacaacaag ccgcagatta tgaacaataa gcgtttctct cgctttttca aagtcatttt
     4861 caagcattgc aagcgttttt gcttcttcct ctttggaaag cggaggaggt aaggtttctg
     4921 ctccgttgat atagtctatt tcgcattttc ctacaccgga catacgcagt ttttgtatca
     4981 gctcgccgat gatctcaatt attttcagca tactttacac ctcacacaag tattttcgga
     5041 ttgaatattg cttcatattc nnnnnnnnnn ctattaccac accgataagg acattaacag
     5101 gatacttttt ttgcctttca tcatttataa taacgctgtc ggctctgaat gtgcagataa
     5161 atccgtcctt tccgactgtt gaaaacggca taatgcgtat gcctttaatc atttccgcac
     5221 acagctccga caagtcctta ctgctgtcta tagccgcaac tgccggaggc gaaatatcgg
     5281 cgcatatatc cttcctgcat actattacag gcagcccgct gaagcagtct acaagtccgt
     5341 ttccgctgtc tgcaagtgcc gacagtatgc accgctttcc gttgttatga atttccaccg
     5401 tatatttccg atcggttctg ccgtttctgt caagtgtgaa ccttattatc ctcattaccg
     5461 tataagcggc acaggtgcat aaaataaccg tcattgtatc tataccgaaa tacacagtac
     5521 cgtttataaa aagcattttc atcggagcgg caaacagcca tacacacagc ataagaccgg
     5581 aaaacaaaaa attcacaaac atcaagaaca aaacactttt catatacttc aaaaagccgc
     5641 cgtaaccgaa cgcagtcagc gttataataa agcacagcgc tattttaagc attgtaagcg
     5701 tgatcaccat atcctgcggt atgaatatgc taagagatgc cgcacctcct attcctgcgg
     5761 atataagctt tctgtaccat atgattttgc ggtgagtaag aacnnnnnnn nnntgtatat
     5821 atttttacgc ttcgtttttt cttgtgagca gcatttccgc attttcaagc gatatcgggt
     5881 cgttttcgtc acccgatatg atccttgcta tctcgtgctt cctgccgtca aagtcgagct
     5941 ttgtaaccgc tgtatatgta cgcttgtcat cgcttgtttt cttgataaga agctgtgtgt
     6001 cagccatagc cgctatctgc gcaagatgag ttacacaaag tatctgtcgg ttattagctg
     6061 cctcgtgaag ctttataccg actttagtag ccgctctgcc gctgataccc gtatctatct
     6121 catcaaatat catagtatgg agcttatctg tatcggcaag gacattcttt atggcaagca
     6181 taattcgcga cagctcacca ccggatgcta ttttgctcat cggtttaagt tcttcaccct
     6241 tatttacgga aataagcatc tcgaccttat ccataccgct cagtgtgacc ttgtcctgcg
     6301 ttatatcaaa cacaagtctt atatctggca tatcaaggaa tgcaagctcg gcagatattt
     6361 tttcggcaag cttttcagcc gctttttttc ggctgtttgt aagctctgcg gcaaggtgtt
     6421 ttactttttc gcccgcctct tttttctttt cggtaagctc atcgattata tcatcgccgt
     6481 tatcgatctc ataaagctcc gtacgccatt tttcggcatt tttgagtatc tctgaaagtt
     6541 ccgtgccgta ctttctgcga agccttaata taacgctcag tctttcttcg taaatacccg
     6601 ctaaacctgc cgtatcttca tcttcatcag gcatatgctg acctagctct tctctgatat
     6661 cttccaactc tacaagcata tcggttattc gttccgataa tttttcaagc tcaggcataa
     6721 tatcaccgag cgatgaaata cagtcacgac agcgttcgag gagcgaatag gcaccatccg
     6781 tatcatcgtc accggatatt gcagcctgtg cattatacag atttccgctg atatattcca
     6841 tattacgcag ctgttctagc ttctgcctta tcgtttcttc ttcatcggcg gaaaaatcgt
     6901 atctgtcaag ctcttcaagt cgttctctga gcagctctgc tttttcaagc gactcagtct
     6961 tcttgcggct gacttccttt atcttacgcg acagcaccga aaattccttg aatgctgcac
     7021 cgtactcttt gagcagttca tcgtttttgc cgtaattatc aaggatttcc cgctgattat
     7081 cactgtccat cagaagacgg ttatcatgct gaccgtgtat g
//
LOCUS       DAWAOG010000030         7046 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1516_length_7046_cov_2.272636, whole genome shotgun sequence.
ACCESSION   DAWAOG010000030 DAWAOG010000000
VERSION     DAWAOG010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7046)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1516_length_7046_cov_2.272636"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3988..3997
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1636 a         1649 c         1448 g         2303 t
ORIGIN      
        1 agttattgct tgccttatcc accatgtagc atacgttgag aacttgtagc ccttagtgta
       61 atcaaacttc tcaactgctt tgataagacc gaggttgccc tcctgtataa ggtcaaggaa
      121 ctgcatacct ctgccgacat atctttttgc tatgctgact acaagtcgca ggttggcttc
      181 gctcagacgc tttcttgcgt acttatcgcc ctgagccata cgtgttgcaa gctcaatttc
      241 ttcttctgcg gtaagaagag gaacacgacc tgtttctttc aggtagattt taacagggtc
      301 gatgccttcg gacgaaagag atgattcaaa gtcatcaccg tcagtaaagt caagagcact
      361 gtcaagatcc tcatcggcgg agaagtcatc gactatttct atattgctgc tttcaaaatc
      421 atcgtacagc ttgtttattt catcggcatc aaagtcaagc tcttcgagta catcggttat
      481 ttcctttgtg gtgagcttgc cgttctgctt accgtgattt ataagttcgt ttattttgtt
      541 gtctgccata tttatctttc ctttcaggat ttatcgttat gattttttgt tctttatttt
      601 atcggcaagt tgccgtaatt cgtccgctga catatctgtc actttctttg tctgtttctt
      661 atctctgtat tttataagta cattcagata atcgttcagc ctgtccacat tgtacggcaa
      721 atcgctgttg tcgtgtatca gcttgtatat tgatgctact tcctccgcag tatatctttc
      781 ggagagtaca gcaacgtcat ttgacagccc gcacgagatc atcgcagtaa ggtcaatgaa
      841 cagacgcttg ttgaagtctg tcacgaaatc gtccgagctt atcttgtcgg tgaccttttt
      901 cagaaagtcg ggggagtgaa ataaaaatgc aattatgccg cgttccgcct gtgtttctac
      961 cggataacgc atagcgtccg gatttatctt atctcttgaa cgagtcccgc cgataatttc
     1021 tctgcggcgg tcgttttcct ttgccttata tcgcctgcga agtatctgat ttacggcaag
     1081 atcaacattt gagcggttca cgttacactc ttgcgctata gttgagatat aaacggcacg
     1141 gtcgatagag ctgtttatat tggcgataaa atccaccgct tttttcagat attcggattt
     1201 tccttccggc gtagtcagat caacagacga acgaagcttt ttaagttcgt attccatgcc
     1261 ggattcactg ttgcctatca ataattcaaa ggcttccttt ccgaaatttt ttatatattc
     1321 gtcaggatct ttagcgccct gaattgtcag gacgtttgcc gtaataccga ctttatcaag
     1381 aatgttcatc gctctggcgg tagctttctg accggcatca tcgctgtcat aagctaaaat
     1441 gaccttttta aagccgagat ttgctatcgt acgtgcgtga gcatctgtta ttgctgtacc
     1501 gcaggttgcg accgcgtttt tgaagcccgc ctggaacatc gcgataacgt ccaggtttcc
     1561 ttcgcacatt atcagcgagc ttcctcctgc gttttttgcg tggttcaggc tgtacaggaa
     1621 tgtactttta ttatatactg ctgtcgcttt agtattaagg tacttcctgt tatcatctcc
     1681 gcctataata cgccccgaaa atccgacaat gttacctctt atatcaataa acgggaacat
     1741 taccctgtat ttgaaaaaat caaagtaatt atcgcttttc gatgattttg aaagcaggga
     1801 agcttcagcc atctctgcat cgccgtatcc gagcgtatgc atatgatttc tgagcgtatc
     1861 ccagctgtcc ggagcataac caagaccata tgtccgtata gtatgatccg taagacctct
     1921 gccgtaaaga taatcaagac cttcattacc ggcgggggag tagagatatt tgtggaagaa
     1981 cctgcccgct tcacggttca tctcaagagt ccttgaacgc ttttttgcgc cgtcgtctcc
     2041 gccgtcctca ggcatcggta taccgaccct ctgtgcaaga aacttgacac tttctatgta
     2101 atcaagatgt tcgtaaagcc gtatgaaatt tataacatct cctgctgctc cgcaaccgaa
     2161 gcaataaaag ctctgcgttg cggtgtatat gtggcaggac ggagtttttt ccgaatggaa
     2221 aggacagtta cagacgtagt ccgtaccagt ttttttaagc ggtacatatg atgaaataac
     2281 atcatatata tcattgcttt ctttgactct taaaagaaag tcttccgata aacgcatcgt
     2341 ttttcctttc tttgcttaca ttgtatcaat acgaccagct cttcgggata caaagctcct
     2401 tgaagtagtc gaccgcaaag tcgtctgtca ttccgctgat aaagtcgcag actgcgcgct
     2461 ccttgccgta ctcctttgca aagccgatat aaagctccgg cattttctca ggagaattta
     2521 taaagtatct gtacagatac tcgactatat ggcacgcctt gcctttttct gcattggtac
     2581 tgggtgcgaa atacacatag tcaaacatga actttctcag ttcatcatgc gcctgttttg
     2641 ttacatcgtc atactgtata aaatccgtac tgttggcgat tatagactta ataagcgttg
     2701 ttatacgctg actctttgtt tcgccgagca cagatatagg acctttcgga agctcttcct
     2761 gcttgagtac tccgcctctt atcgcatctt cgatatcgtg atttatgtat gcgatcttat
     2821 cgctgaactt tacaacttga ccttcgagcg ttttagcggt tgcgttggtg tgacaaagaa
     2881 taccgtcaat gacttcgtct gtaaggttaa gacctttgcc attcttctcg ataacttcaa
     2941 ccacgcgctt actttgctca taatgtcgga aatgtccggg gataagctga tcaagcgtac
     3001 gttcgccgtc gtgaccgaac ggtgtatgac ctaaatcatg accaagcgct atcgcttcgg
     3061 taagatcctc gttgagtcgc aacgctcttg cgatcgtcct tgcaatctgt gatacttcaa
     3121 gcgtgtgtgt aaggcgcgtt ctgtaatgat cggatttcgg tataagaaaa acctgcgtct
     3181 tatgcttcag tcgtctgaaa gagttgcagt gaataattct gtcacgatcc ctctgaaagt
     3241 ctgttcggat atcgcaggga gtttcgtata cctttctgcc ttctgagtct gtgcacttgc
     3301 aggcatatgg cgagagcgtc atattttcta tttcctcggt aatcatacgg ggtaatttat
     3361 ccatactgaa cacacccttt catcactgta tgtgacaaat cgaagatgag cgataccgga
     3421 tcgttcatct tcataaataa tatactgagg atttttataa aagtccttta attttttaaa
     3481 aatatttaaa attcgacata gccgttgctg agttttttta tatccacctt accgttacat
     3541 ttgtcgataa gttttgaaat gagctctgcg gtgagttctg ccttgacatc tgcatagacg
     3601 catacaatgt cggaatactc ggtattgcgt ataattgctc cgtattccgg taggacagaa
     3661 cctattatac cgtacatggt gtagtcacag cttatcttaa gactgtcggc gacttccatt
     3721 gttactatat cagagtttgc aagggcagtt gacgcgcctt ttgaataggc atgaacaagt
     3781 ccccctgtac caagcaggat gccgccgaaa taacgggtaa caacgcatac aacgtcggta
     3841 atcccgtttt tggacagcac gtcataaatc ggcatacccg cagtgccgcc cggctcaccg
     3901 tcatcgctgt gcctgcttgt gttattatta cggagaatat aagcatagca gttgtgagtt
     3961 gcttttcgat gcatggctct tatctcgnnn nnnnnnntat ctcgtttata aaagctatgg
     4021 cttcttcctc tgttttggca ggggatatgt agccgatgaa ctcggacttc ttctcggaaa
     4081 acgaagactc ggcacgcttt cttattgtct tatagcccaa tattcgcccc tatcagcctt
     4141 ttgtgccttt taatgcggca cgggtctttc ttacatcggt cagattaccg gcaagaagtt
     4201 cgtcaactct gctgagcata ttttcaacat agtcgtttgt ggcgtttctg atctcctttg
     4261 acttggtctg tgccgcagtg agaatttcat gtgctctctg ctgagcctgt tttgtaataa
     4321 cctcttcgga aaccatgacc tttgcttttt cttcagcctt tcttataatg ctttctgcct
     4381 cagctttagc atcgtttatg ataactctgc ggtcatttac aagtgcctgt gcctgcttta
     4441 tctctccggg gagattaagt cttacttgct ttacaagctc tctgaactgc tcaacatcga
     4501 tgagagatct gcctcctgta aggggaatgt ttttcgacct gtcaagctca tcatccattg
     4561 cttcaagtat ttcttcaatg ctcattttaa aatcgtcctt tcatttataa catttgtttt
     4621 ttatgctcag tagcgtttca gtctttcatt tattctgtca agtatgcatt cgggaacaaa
     4681 atcacttata tctccgccga acatagcgat ctgctttacc atactggagg acagataggt
     4741 atattcgctc tttgatgtaa gaaaaatcgt ttcggctttg taacaaagct ttctgtttgc
     4801 ctgtgccatc tgaaattcat attcaaaatc cgatatggct cgcagacctt ttacgatcac
     4861 gtccgcattt ttttcattaa agtagtctat aagaagtccc tcgttgctgt caacctcaac
     4921 attaccgata ccttcggcaa ccacgctttc gcgcaggaac tgctttcttt cttctattgt
     4981 gaaactgcac ggcttgagcg gatttaccga cacaagtact attaccttat caaacagctt
     5041 tgatgctctt gaaataattt caaggtgtcc gtatgtcacg ggatcaaagc taccgggata
     5101 tatagctgtt ttcataattt attcctcttc ttccgtctgt ggttttctgt agaaagtaag
     5161 actgcttttg ccgtatttct tcgattttgt gataacaaat ccgtttactt cttcgggcag
     5221 tctgcaaccg ggctcgtgct cgcacacgat aacgcctgtg tcgttcatcc tttccgtgag
     5281 cgcgggaaga gagcgctgta tcaggctctt ctcatacggc gggtcaagca gggcgatgtc
     5341 aaatttctct cctgtggttc ttaagaatgc ggagttcggc atattgacaa tttcacactg
     5401 actgtcaaaa ccgacgtgtg atacattctg ctttatgaac tttatcgaat tctgactgtt
     5461 gtccacaaga tacgcctttt ttgcgccgcg gcttactgct tcaattccga gctgtcccga
     5521 gccggcaaac agatcaagta ccgtgcttcc ttcgatctca aactgtatag cgctgaatat
     5581 cgcttctttt acactgtcct ttgtcgggcg gacatcaatt ccctcgggcg cgatcagctt
     5641 tctgcccctg gctgtgcctg ttattactct cattgtattt cctttctttt gcttgatcaa
     5701 aaattctctt gtatataatt gtatatttct gttgcttttt cttcttttac ttttgcaact
     5761 gccgccagct catcgggaga agcgtcttta agcgcctgct ttgtcttgaa atgctttata
     5821 agcgcaagcg cttttgcttc gcctatgccc ttgatttttg taagctccag ctcgtatgtt
     5881 cttttcttgt gcgtctgttg ctggaacgat aacgagaagc gatgaacctc gtcctgaata
     5941 ttggtcataa gagagaaaag ctttttatta gcgtttatct gtatctcttc tccctgtgcc
     6001 gctatagctc ttgtgcggtg tttgtcgtcc tttacaagtc cgtacagcgg tattgaaacg
     6061 ttcatattat caagcacctg ctgaacagcg tgaacgtgac ccttaccacc gtcgagaaga
     6121 atcaggtcgg ggagcggaga gaagttttcg tcaccgtcaa gatagcgctg tatacgcctt
     6181 tgcaatacct cttgcataca agcgtagtcg ttctgtccga taacgttctt aatggcaaac
     6241 tttctgtagc cggctttata aggtctgccg ttgcggtata ctatcatacc gccgactctg
     6301 ctgtcctctc cgagatttga tatgtcgtag ctttcaataa ctgtcggtat cttagacaag
     6361 cccagtaatt ttgcaagatc ctctagcgcg tttatctcct taacggttct gcctaccttc
     6421 atcgacagat attcggatgc gttctgcttt gcaagaagta ctcttgacag tccctcgccg
     6481 cgttgagcgg cagtgatggc aactttatgc ccgcaaagag cggagaagta ctcttcaagt
     6541 accgcgcggt cttccatctc cttgtcaata tatatctgtt tcgggattgc atcggttctt
     6601 gccgaataat attctaccag aaaatcagag cgcatctgtc ccgggtcgta ttcatcaccg
     6661 atgaagaaat ttactttgtc ggtaattcgt cccccgcggt agcatatgac agctatactt
     6721 gcaagttcaa cattctgcgc cagtgcaaca acgtcaaaat cataggtctt gtcagaaaag
     6781 atatcctgac cgtctttcat cttctctatt gcatttatgc ggtcgcggag ctttgccgct
     6841 ttttcaaact caaggttatc ggacgcttcg tacatctgct ttgtaagctg ttcaacgctc
     6901 gctttactgc cgttttgcat atactctatt gcagagcgta tcatgctgtt gtactcatcc
     6961 tgttttatct tgcctgtgca tacgcccatg catcttttta tatgatagtt aaggcatgga
     7021 cgttcttttc tgaaatctct cggaaa
//
LOCUS       DAWAOG010000031         7009 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1525_length_7009_cov_4.170549, whole genome shotgun sequence.
ACCESSION   DAWAOG010000031 DAWAOG010000000
VERSION     DAWAOG010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7009)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7009
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1525_length_7009_cov_4.170549"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2884..2893
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1410 a         1693 c         1742 g         2154 t
ORIGIN      
        1 tgggcgcttt ttattagatt gaatctggtt aacccatatt aagactaatt ataaaaatta
       61 atataccata ttcagacaaa atatgcaatc ttgcctattc tttgagtttt cgtactttta
      121 tccacactcc cccgcactta aatcttatct tacggccatc cgtaacggac tacgcaagaa
      181 gctgtcatac acaccgatga cagcggcatt gtaaagcctg atggtttcaa agctgaaaaa
      241 aggctctcca acataagttg gggagcctga ttattgttct tttacttttt ctttctactt
      301 aaaacaactg ctactgccgc agccagaatt attaatgcta ttagtccggc taccgcagcg
      361 cctgtgacgg ggttggtttc gtcgttttcg gacttttcag catcaccgct ttcgttgtct
      421 gcggcgtggt cgttttcgtc aactattacc ctgccgccct cgtccttgat gaacggaagc
      481 tcagcgtcct caggcggaag cgtggttgtg gctgaggttt cggacggagt tgtagttgtt
      541 cccgactgag ccgaagtggt ttcggacgga gttgtggttg ttcccgtcag atccgaagta
      601 ggttcggacg gagttgtggt tgttcccgac ggagccgaag taggttcgga cggagttgtt
      661 gttgttgttc ccgacgaagc tgttgcagga ataaccgtgc ctcgcttgac aagttcattg
      721 cataccgtgc agtattcatc gcctgtgtag ccgttggctg tttcggtcgg ctcaactctg
      781 tttctgattt cggttgctcc gtgtcctgcc gcaggaataa caatgtcagc ttctgtcagc
      841 gccgttgcag cgttgctgtc ggcaaacatc ttgccgcagg agcatacata gtagccaata
      901 ttgccgtcct ttgtgcaggt aggcttaaca gcataaaccg gcataaggtt gttggcatga
      961 atgtgtccca gcatcggagc gatttcgtat ccgcagattg tgcaggtttc agcggcagtt
     1021 tcggttgcat gaccgcttga aacgtgcggc tctattttat ccacagagcc acagacatca
     1081 catttatgcc aatgcgagtc gctgtcgaat gtccatgacg tgaggtagcg gtgtcccttt
     1141 gccgcaatca ccgtatcggc gtaagttatt tcgtttttgc cgtcagcgtc tgtgaacagc
     1201 ttgctgcagt taccgcattt gtagtattca atgtttccgt cggttgtgca ggtttcaggg
     1261 acataatcga aatactgcat attgtgaccg agaggttctc cgtattcgta gccgcaggct
     1321 tcgcaaatac ccttatccgt gcaggtcgca ttaccgccaa ggtggttttg atagttaaac
     1381 tttacggtgc agaggtcgct gtttttgcag acataccagt gcgcggtttc gttgtagcga
     1441 tagccgttct caacatccgc ctcgcaaagc ttgaacactt taaataagtc gttgaagtgg
     1501 taatcaatct gtgttgtcgt gccgtctgcg tctgtgaatg ttaaagaatg gtcgcgatct
     1561 gcaagccata agtgaagtcc tgtgtcgttc gggtcggcgg caatattgtt ggcggcaatt
     1621 gtcataacgc cgtcaacgat gacttcatcg ccgttaggat tgtcaactgt cagcaggtaa
     1681 acaggttcgc ctgcgctgtt ctttatctgg ctgttctgag aaatatcggg gctgactgtg
     1741 cttgtcgagt acatagctac cgagctgttg tcgatataaa tttcgcttgc aacgccgtca
     1801 acgccgtttc ctatcatatc aggtccgcag gagcctttct gaaccagcga aacaacgctg
     1861 ttttcgatat agattttgtc gcctgttccg tcgacgccgc cgccgataat cgctgcgccg
     1921 gattggcttg ctgcgtaaag ggtcgagttt tctattctga tgtttttgcc gtttgcgttc
     1981 tgaccgccgc cgattgctgc gctgtaatcg tctgttacgg cttttacatt ggcgttcgca
     2041 attgtaatgt ccgaggcgct gctttcctta ccgccgccga ttgccgccgc gccgaagccg
     2101 ccgactgcgt tgatgttgcc gcctaaaatc tttatatttt cagcggttac agcttcggag
     2161 gagcttgaat ttttttcgcc gcttccgatt gccgctgaag agccgccgcc ggtggcgttg
     2221 atattgccgc ctgaaacagt gatattctct gcgcctgtgt aaataccgcc gccgattgct
     2281 gcgccgtgct tagaagccgc ggtgatattg ccgcccaata tcgtaatctt ttgagcagtt
     2341 tttccgttgt actgaccgct ggttgagccg attgcggagc cgtacatatt cgcctttgcg
     2401 ctgagcgtgc cgcagttttc gtcacacttg tgagaaaaat cgtttttagc cttttcgcaa
     2461 gttattgtca atgtgcctgt tccgtctttt gcaatgccgc agccgtacat actgtcagag
     2521 ataagagtgt tggtagttcc gtctgcaagc gtgattgacg cggtgttgtc attggctatg
     2581 cagagcgctg agctgtcggt ccacattgac gggtagctga tatttactcc cgcaagggta
     2641 acgtccgcat aggacgcatt gattacgatt atgtcctgtg ttgaggaaac gccgtctgcg
     2701 ttcttgatta caacaggagt atttgtgtaa acgttcagcg ttcctatatc ggcggatacc
     2761 tgcgtaaccg ggttcgttac agaggagata taatagtcgt aatcaacgcc cggggtaagc
     2821 gccgagccgt cagccgcagt gataacaagg tcgcttccta tggctgcaaa ggcgcgcatg
     2881 gcannnnnnn nnncatggca gcaatgtatt cgtatgtttt ttcgctgccg ccgaccttaa
     2941 cggtatgctt tgccgagcgg tcggcgtaaa tgtagaggtt agtgtcggaa tcgctgtggg
     3001 ttttgaatgg cagaagcttt ccgttaaagt aaacgtcctc attctgtggg ttggctattg
     3061 taataagagc aacctcagga tagctgccat ccgcattttc gctacaggaa acgggagctt
     3121 tgctgattgc gtttatgccg taggtctgtt cgccgtatgc cccgtttttg ccgcggatat
     3181 atacggaagc ggagctgtct atatgaacct cgccgtcagt gttgtttgtt gtagctaaag
     3241 cgtaatatct ctgattgctt tcaagggtgt aaacgccctt tgaaagggag aaatattttg
     3301 cgtttccgag agcggaggca ccctcgctct ttgcgtagat aaatccgcct gagaggatta
     3361 tttttccctc ctgatagccg tctattgcgt tttcatcgcc ggaaataacg gttatcctgc
     3421 cgcttttgaa ttcgatgaac ttatccggct cggaatcaag tatgccgata gcgggagcta
     3481 tgtcttcctg ctcgacataa agagtgccgc aggtttcatc gcagatgtga ccgtattcgg
     3541 cagagcctgc atagcagctt acagtcaggc tgcgtcctac tgagattgtt gtttcgctgt
     3601 ctattgctac cagcttgttt gtgctgccgc cgtcgattat aagattgcag gctacatatt
     3661 cattgcactc gattgcaggg tggtacgtac cgctgtcgct gtcgcaaggg gcgtaaatgt
     3721 ttacgccgtt aagggtaacg ttgacggttg acggcgcttc atcgctgccg atatagatac
     3781 ggttgtttgt gggaacgctt tcatcggcgt tctttatggt tacgctgccg gcgcagttgt
     3841 ttactgtaag tatgcccgtt ttggcagggt aagtgtattc taatccgtat tcaaggattt
     3901 cgttgtcgcc tctaataaca aggtcggatc ccatttcctc gaatattcct tcgttaagac
     3961 cgtaggaata ctgcttgtcg cctaccttta cgcttgcagt gcctgctata tccttgggaa
     4021 cgtaagcata aaaggtcata tcggagctgc tgaggaatct gtactgctga gggtcgccgt
     4081 ttatagtgat ttcctcgccg ttcgggttgc tgattgcggc aagatagacg tactcgccgt
     4141 tattgttttt gagtttagcg tcgctgctga acggcttcag gttcttagtc ttgtcattta
     4201 ccagataaac cataccgccg tttatttggt agttacgggt gttttcggaa tttgaccagc
     4261 cgccgagcgc gtagctgttg tagccgccct gggcggttac gataccgtcg tttataataa
     4321 tgttgtctgc ctgaccggtt gcgcctgaac cgatcgcgca gccgtaagcg ttattattgt
     4381 taaagtaggt tataacagcg ttgacaatac cgccgttaat gtaaatgccg tcagcgctgc
     4441 ctccctgacc gctgccgatg gctgcgccaa agacgctttc tgcgttgatt ataccgccgt
     4501 tgacggtaat attgtttgct ttagcgttat aaccgccgcc gatgcctgcg cccttgctgc
     4561 cttttgcatt gattctgccg ccgtttatga caatgttgct tgcggatctg ttatcatcgc
     4621 cgccgatagc cgcagggtag ttagagcttg ccgcattaag cccgccgcag ctactgccgc
     4681 agatatggcc ggcttctccg ctgtgctggc atgatatggt gagcgttccg cattcggcac
     4741 tgtctgagtt cttggacagt gcggcgcaat tataggttgt ggaaataata ttgtccatgt
     4801 tgtcggcaag gattatgttt acatttccgc tgctgttttc ggcaatttca acagcgctga
     4861 gttcattcag acctctttgg atatttattc ccgcaaaagt aatgtttgcg gaaacgccgc
     4921 tctcaatgta aatgtagttg ctgcacggct gagttgggtc aacatttttt atggtaatgc
     4981 ccttgtccga taaaattgtc agcgggatgc cgtcaatttt atagtctgtt ccgtagacaa
     5041 gtggtttatt gctgtctgtt gcatagaagt cgagcttcat atcttcagcg tttaccagac
     5101 cgctttcggt gtaaatatag tcggtggttg tattgcctac cgttacgctg tttctgccgc
     5161 ttttcggaat gaaaacgtag gactttttaa cctctgtcga attatcaaaa ccggtgttgt
     5221 atgtgctgtt tgacggatat gcttttctgt ttacggtaat gctttcattg cccgaattgt
     5281 ctatttccag taaagtcagc tctctgccgc ttgagtcctt cggcagggag gacataccta
     5341 ttttgtcgga atgtacggta cagtcgtcgc cgcacttcgg atataccgag ccgccgtcta
     5401 ttacgatgtc ggcagggtct gctccgctgt cggaaacgcc gattgcgtca gcgctgccgc
     5461 tgccaactgc gtaaacaacg ccgtcggaaa ttctgattat gcctgttctg tccgagttgc
     5521 agttgtttcc tgcgccgaca gcgactgcgc ctgtgccgtt gctgtcataa gcggtatttg
     5581 cgtagatagt gccgccgctt atgtttatgc tgtcaaatct gctgcagtaa gcagcgccta
     5641 ttgccgcgcc gtaaaagtgg ctgccgctgg aatttctgaa tgcactgccg tatgccgtaa
     5701 ttatgctgca gcttatgttt atagagccaa tgccgccgtt agtggcgctg ccgatgcctg
     5761 cggcgccatt atctccccat gcttcgatta tgccgcccga aatgttgatg tcggatacat
     5821 cggctaaatt gctgccgccg atgcctgcgc cgtcagtgcc gacagcttta atctgaccgc
     5881 cttttatgta tatgccgccg gtttcggttc cataattgca tcctgaagcg cctatgcccg
     5941 cgccgtgtcc cgcgctgcac cttgcgtcaa gcataccgca gttcatgtcg cactgatggt
     6001 tttcttcgtc tgcgtgttcg caggtgattg taagccttcc gttttcggac agcttttcaa
     6061 tgcctgccgc gttcacgccg ttcagatagt tggaagtccc acctttaagg gttatggtta
     6121 cgttcgcaga gctgtcgccc atggatatag cagcgccgtc agttgttctg atgcgcagag
     6181 agtcaagcgt aatattcgcg tcaacgccgc ttttaataaa gatttttgcg ttgacaaatt
     6241 gattgtttac gctctttatt gttattggtg tgcttttcag aatggtcagc aagctattat
     6301 tttcgtcata gctgtaatct ccgttcgcag cggttcctcc gcttataaga aatatgccac
     6361 ctacattttc tttagcttct ccagcgtctg cgctgacgga cagggttccg cctgctatca
     6421 ttgccgaaac agtaagggcg caagctgcga gcaatgccgc aatcttggat tttctaccat
     6481 gtttttgttt cattttgtta ccccccccta ttttttctct tttgccgcag ttttagtgcg
     6541 gaatttatat attaattacc aatttaatct tgccatgtaa cggcttgttt tgtcaatagc
     6601 aattcccttt gagttttcgt acttttatcc acactccccc gctttcacag gctctgctat
     6661 aaacaactgt gaactaccca ttgtcttaag ccaatgggct tcctgcttca atcgacctcg
     6721 taacctaata tctccacagg cgttaattcg ggcagtccct gccctattta ttattttttc
     6781 ttatgctatt tgtcttagtc cttctgcaag tatatttttt gcagcattga tatccctatc
     6841 atgtcctgct ccgcatttcg gacatatcca tgtcctcact gacaaatctt ttgtatctga
     6901 attctgatac ccacaaacag aacataactg actgctggca taaaatgtat ctatatctct
     6961 gtcctactga tactgccgtc attgtggcaa tcttcccgtg gcttctgtg
//
LOCUS       DAWAOG010000032         6995 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1532_length_6995_cov_1.970749, whole genome shotgun sequence.
ACCESSION   DAWAOG010000032 DAWAOG010000000
VERSION     DAWAOG010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1532_length_6995_cov_1.970749"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1108..1181
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5013..5022
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1887 a         1705 c         1401 g         1918 t
ORIGIN      
        1 atatcaacgc tcatatcggc gcaacttcac aggactgcca gaagggcaga cagctcgttg
       61 atgaaactgc cgaatatata ggagcggcga acgaaaagat gcaaaatctc acagccgcaa
      121 tgcaggagat aaatgatgct tctaccgaga taggtaaaat aatacagacc attgaagata
      181 ttgcgttcca gacgaatata cttgcgctta acgctgcagt cgaagccgca agagtcggcg
      241 aagcgggcaa aggctttgca gtagttgccg atgaggtcag aaacctcgca ggtaaatccg
      301 ccgatgcagc tcacgatacg accgttctta tcgaacgtgc ggttgccgca gttgaaaacg
      361 gaaacaatat cacaaacgaa acggctcagg ccgtatcaga ggttgaagca agatccggcg
      421 gtgtcagcga tatagtaaac aagatcgcag ccgcaagcct tgaacagtcg gatatggtaa
      481 agcaggtcaa catcggcgta gagcagatct caaatgttgt tcagacaaac tctgcaacag
      541 cagaagaaag cgccgccgct tcgcaagagc tttcagctca ggctcagaca ttgcaaaagc
      601 ttgtcagccg ctttacattc agagaagaat aaagcatcaa aaaacctccg gacgtatcgc
      661 agtccggagg tttcagcttg tagaaaacct ccggacatat cgcagtccgg aggtttttta
      721 tatattcata tactatgact ctgaaatgta tttagagagc gccgaaattc aagtgtttat
      781 aagctctcat accgatttca attttaatat cggttatcac ttccttaaca gcttcttata
      841 aagaaaacct gttatacagc tcgggattat aaacagaact atctgacctg cacacggaat
      901 aagaaaatcc gcaaagcttg aaaatcccat ttttcttatc ttttttctga ccgcgataaa
      961 gctcttggtg ttgcggaaat ttctgcggcg gttcaggttg cccggtgtaa ctctgtacct
     1021 tacaagtgcc tccggaatgt tagcggcttt agcgcccgcc atcagcatac gaaccataaa
     1081 gtcgtaatcc tcacaacgct caagctcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtatccgcc tattgataat
     1201 gcaagtgact ttctgtacac aagcgtttga ttattgaaag gggctctgcg ccttgcaaac
     1261 ttgcgtatct cgtcattttc gaccggcggg caacgcgttg ctatatcttc tcctgtgccg
     1321 ctgttgaact cctgaatata acagccgagg atagaaatat ccgggtgttc ggcaagatat
     1381 ctcatctgct tctctgctct gttatcaagg cagatatcat cggagtccat cttcattata
     1441 aactcatttt tgcaaagagc aagccctgtg tttgcacatt tggcagtacc gccgtggctt
     1501 tcaaggcgat taactaccag tcttccgcca aacttttcac tgtataccgc aataacatcg
     1561 ttaagctcct tatcaagagc accgtcacat acaagcacta cctcatcggc aggataagtc
     1621 tgtatataca gactttcaag gctctgctca aaaaatgcgg cattttcacc gccgtataca
     1681 gacataagga cgctgtacgg ctgtaattct gttaaatttt ccatacaaag cctccttaaa
     1741 tatcattctg tctgctgtaa acgcttacat agattttatc acacagcaaa gtaaaagtca
     1801 atcataaaca acatttttta tccggttttc tccgtattgt catattatca gtcgaaatac
     1861 aaagctttac ggtaatctat ctgacctgtg aatgggtcgt atattatccc ctcgcagttt
     1921 ttagtgctga taaaatactc agtgacatat gccaccggta taaaacgtcc gtctgttatc
     1981 agttgctttt cagcgttgac acaaagctcg tattcctcct ttgcatcctt tgcctgggtt
     2041 gcctgctgaa gcaaggcgga atatgtatct tccatcgcac tgtacttata tccgctgtcg
     2101 gtaaactgcc tgagatatgc ctccgggcgg ttgtagtcac cgtttattct tgcaagtgcc
     2161 agcgtataat ctccgcttgc gattttctca tcatattcgc tttcgctcac atatgagata
     2221 ctgcaataga agccaagctt tgcctgccac tgctgtaaaa taccgccgag cgattcgcct
     2281 atctcctcat cacggttctg cacagcaaga atgttgacgc tgtgaaggtc agccttgtcg
     2341 atattttcgc aggcgctctg atatgaggaa tacgccttta tttcatcggg ctttacagca
     2401 gttctgtttc ctgctgtatc gcgatagccg ccgagagaat ttttaaccga cggagctacg
     2461 atcccgtcag ctgcggaata gccgaacggc agactgagcg ttgattatcc gatgcaaaaa
     2521 gcaacgcata tcttagctta tcgctcttga agatcgactt cttcgtgttc atcataatgc
     2581 catatacggc ggtcgaatac tcgtcatata catagtcgtt tccgagataa ttctttgcgg
     2641 tgctacccga aagaacgatg ctctgcgaag ttccgtccga aaaatcagtc atatgatcat
     2701 cttcgacaaa gaagttaaga cccagcggcg ttacctcatc atgctcatcg tatttgtcgc
     2761 tgtagcgcag taccatattg ttattatttt tgctccacgc atcatagtac cacgtcttta
     2821 cataaaacgg accgtttgac ggggtacgct cagcgtcaag accgtacttg ccctgtgccg
     2881 ccgcgaagta atctttgttg cacggcattg ccgccggcat tgaaagcatc atttcaaatg
     2941 acggctcggt gtattcaagc tctattatga gtccgaactt tccgtccgcc ttaactccga
     3001 gctgtgatac atcggtaatt tcaccgctgt ttatcttctg cgcattcttt atgcagaaat
     3061 attcggacgc tttaggcgct ctggtttctg gcataaacag gcgctgaaaa ccgaaaacaa
     3121 aatcatctgc ggtcacctgt ttattgtatc cgttcacatc tgtccaatat ctgttttgtt
     3181 taagtgtaaa tgtatatgta agcccatcgt ccgatacggt acagctttca gccatagctt
     3241 tttcgacact gccgtcaccg gcggttctgt acaatccctc aaatatacag ccgattatga
     3301 gatacgactc cttatcggag gcatactgag gatcaagact tcccggattt gaggatatat
     3361 cgtatccgaa tacatatccc ttgccgtcat cttctttaca cgatgtcatt gtcaaacacc
     3421 cgatacacag cagtaaagcc gtaatacacg ctgtaagtct tttcttcatc aaagctcctt
     3481 aattacaaca ctgccgcttt caatcgactt ccgacagtcg attattctct gatttttgct
     3541 tcctttgaaa cgcagctcaa ggcttctctc cgaaaggacg aattttccgt caacgagtat
     3601 atctactacc gacagcagat ttttcacatc ttcgtctgtc tttgattttt caacaagctg
     3661 ttcaaaggta aagcctgtat atgacattat atcaagcttt gtgttttctt ttatcttcct
     3721 tgcaagctca tacaagggtg ccggctgaca gaacggttct ccgccagaaa aagtcacgcc
     3781 tttaagcagt ggattagcta ctatatgagc atatatctta tccgtatctg caagccttcc
     3841 gccgttaaaa tcatgcgttt ccgggttgtg acagccctcg caatgatgag gacagccctg
     3901 tgtaaatata gtatatctta ttcccggtcc gtctactata gattcgctga ccgtaccggc
     3961 tattctgatt aacattgtat cactttcctt tttgcattat cctatttatt atagccttat
     4021 tcagagaaaa aagcaataga ttgaaaacgt ttcccgaatt tttcacataa aaaacaggcg
     4081 gttgatttcc gcctgctata ttaccttgcc gttttcacgc cgactctccg ctttcctgct
     4141 tctctctgca ttcactgcaa atgcccttta tgcttatctc atactctgta acatcaaaat
     4201 catacttagt gcgtatcttg tcgttaaggc acatcatttc gccgagctca acgtcaaaaa
     4261 ccttttcgca gcaactgcat atcatatgaa aatgcggatc tgtcctgccg tcaaaacgca
     4321 cacttccata agaactgcat attttcttta atatgccgtg atcggcaagc tgatttaaat
     4381 tgcggtaaac agtaccaagg cttattttcg ggtaactgtg ccttgctata tcatatacct
     4441 gctctgctgt cgggtggata ggatttgaca caacgatatt cagtatacag tttctctgtt
     4501 gtgaatagtt catactgccc tcctatcagg tataactctc gttatccgtc acccaatgac
     4561 ggactctgag cggtatatca agcccctccg caagctcctt gcattgttcg atctgctcag
     4621 gcgttattat atctaccacc gtgaacataa ccttcggaac aatagtcttt accgtcttag
     4681 caaaattcag catagaattg aatgcgggaa gtccgaactt gggacgggtg acctcaagat
     4741 agctctcagg gtcgggagca ttaaggctga tagagacaca gtcaattaaa tagcgcatct
     4801 tgtatgcgtc aaaagcggga tttatgaagt caaccagtcc gtttgtattt atacggatct
     4861 tcatatccgt agtctgcttg acatactttg cgacctcgag cagaacgtcc gcacgctcca
     4921 taggctcgcc gtaaccgcag aacacaagct catgcagtcc tgtcttatca aaatcgtcaa
     4981 acgccttgat tatttcttca tagctcggct cannnnnnnn nntataacat aaagacaaaa
     5041 attagtcaat agaaaaaacg gcttttcaga gccgaaaaat aactctgtcg gcgattatcc
     5101 ccgcattctg ttgtatatcc cgtcggattt tacagtagta accgaaagag ggaaatatac
     5161 ctttgaattt ctgctttctt taatctgagc cagtgctgct ctgcacctcg gacgcaagtt
     5221 ctcattgtta tccccgagct tttcgatata aacagccgct ccctcggaca aggcagaaag
     5281 actgtaagta tcgatcttag ttatattgcc gctgagatag ctttcgacat tatactgtgc
     5341 tattctcgca tcggggtcgc aaaagcacag tgcgccgagc attgcggtaa acgacacaaa
     5401 aagtatcaac acggttttca tatcaggtaa tatctcatgc aatatcagca ctaagaacac
     5461 tataccgagc agtatcataa accatgatgt gtaaaatctg agtccggtaa gcccgtactc
     5521 accgatgtac attatcatct ttgacattgc actgcaaata agaaaaacgg tcagcaggct
     5581 gaacacagcc gaatacgctc ttacagccgc aggctttctc ccgctctcgt ttcttttagc
     5641 aagcactcct gcaaggaata tcacaaaaag atcaagcaca gccactccgc acagctcaaa
     5701 aaagccttgt ctggcatatc cgctgtagct gtagccgtcg ggaagaactc cgctgaatgc
     5761 actgagaaaa tatggtatct ggcaagcgaa gaatagaaga tatatagcta taagcggcgt
     5821 gagcgccgcg catacagccg cggcaggcac ctttgctgct ttcccgctgt cacctgtcat
     5881 tctgcgcttc cttccctttg cgccgctcat tattccaaaa agcagaaatg aaaaaggtat
     5941 gctgactgca aaaattataa tattactgaa aaagttgttg aaaaatccgc ctgtcataac
     6001 ggataacatt ctgccgaaat tatcatcggc gcttgaaagt gccacacccg caataagcgt
     6061 tgccggaagc gagcaaataa ttccgagcag gatatacagc gcggttttgc tcttgtgctc
     6121 cttttttgaa aacggcactg ctatgctctt aggcgcggct gtgaaatttc tgaacgatac
     6181 cgagaatacc gcttcgcata gctcccttgc aaatccgtcc gaaaacagcg gcttggttgt
     6241 agtaaagctg tatgcaaagt aacaaagcag gatcgcggca tacacccacg aaagaaagcg
     6301 cacaagtccc gttgacgaga atatcggaac agcacagaat attatcacac agccgaatat
     6361 cgcccactgt gaggctgtcg gctttttgcc gatatatatc accgccataa cagcaagagc
     6421 ggcaacaata atatgatacc agcccgatgc tataacctct gcaaacggaa atatctcgcc
     6481 ctcggtaaag aacagcttaa atgttataaa gccaagcaca gccgtaagcg cggcgaatat
     6541 aaaatcgcgc atattgtagg ttttctttgc ctcgggagga ccttgcaggc tgttatacat
     6601 cggtgcatat tgaccctctg ctctgccgta tacattcacc ggctgaacat tgccgtattg
     6661 ttccgcatta ttatactcat tcattttcgc caccgccttt ataacacagg atatcaccgg
     6721 gttgacactg taagacctca catatctttt caagcgttga aaagcggata gcctttgcct
     6781 tgcccgtttt cagcaccgac agatttgcgg gggttatgcc gatggcggcg gcaagctcat
     6841 tcgacgagat cttccgcata gccatcatta catcaagatt tatttcaatt gccatatcag
     6901 tacctcacaa atcagatagt aaagtcgttc tcttctttta taacgatcgc ctgctcaaag
     6961 acattcttga taacacggag tatcagcccg aagaa
//
LOCUS       DAWAOG010000033         6918 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1556_length_6918_cov_2.355967, whole genome shotgun sequence.
ACCESSION   DAWAOG010000033 DAWAOG010000000
VERSION     DAWAOG010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1556_length_6918_cov_2.355967"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2062..2071
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4709..4718
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5162..5171
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6583..6592
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1792 a         1609 c         1554 g         1923 t
ORIGIN      
        1 gcagacagca atggagctgc tcggcaaaat ggggcttgcc gacaaggcaa acgaatatcc
       61 gtgcaatctg tcgggcggac aacagcagcg tgtatcaatt gcaagagcgc ttgcactaag
      121 ccccgagctg ctgttctttg atgagcctac ctccgcgctt gaccccgagc ttacgggcga
      181 gatattaaag gtaataaaga gccttgcgga agaaaagatg actatggtta tcgtaactca
      241 cgaaatgcaa tttgcaagcg agatttccga ccgtgtaata tttatggacg gcggatatgt
      301 tattgagaac ggcactccca acgaggtatt caacaacagc cagaacgagc gtacacgtca
      361 gttccttgaa aggtatcatt cctgatatta cttgacgatg aaccgcaatt atgataaaat
      421 actttttata cgtttacaca gacagtaatt atatggaagg aaactaatat gaaggtactc
      481 ctgttttcgg aaggtatgaa ccttatcaag atatccgggc tcggcagagc gataatgcac
      541 cagcagagag ctatggagct taacggcata gaatatacgc tcgaccctaa ggacagctat
      601 gatgttgttc acataaacac gctcggactg aaatccaagc atcttgcaaa aaaagcaaaa
      661 aaggcaggca agagagtggt gtaccacgct cattcaactc aggaagattt ccgcaattcg
      721 ttcatcggct ctaatctcat ttcggggctt ttcaaaaagt ggatatgcag ctgctaccgc
      781 aggggcgatg ttatagtgac cccgactgag tattcaaagt cgctgataag aggctacggg
      841 ctgaccgcac cgatctatgc cgtatccaac ggtattgacc ttgcgagata cgagcctaaa
      901 gacggcgaca gggaagcgtt ccgtaaaaag ttcggcctta aagataacga taaggtcgtg
      961 atctcggtag gactttactt tgagcgtaag ggcttgcttg attttgtgca gatggcaaag
     1021 gatatgcccg actataagtt tatctggttc ggcaaaactc cgctttacag cgtaccgcac
     1081 aagataagaa aggcggtaaa gacaaagctg cctaacctca catttgcagg ctatgtacag
     1141 cccgatgagc tcaaacaggc atatgtcggc tgtgatgtgt atatattccc taccttagag
     1201 gaaacagagg gtatagtgct ccttgaagcg cttgcggcaa aggcaaatgt tattgtccgt
     1261 gatatacctg tattcgactg gttaaacggt ggtacggatt gctataaggc aaaggataag
     1321 gacgaattcg agcgtatgat aaaggagatt tacgagggca agctcccgtc actgcgtgag
     1381 aacggacgca aggccgttga aaatatggat ataaagaaga ccggcgaaaa gctggtgaag
     1441 atttataacg gcaccgccga gggcggagat tgataaaaag cgccaagata ctctattgca
     1501 taaaaaacgg acactcccaa aagagtatcc gtttttttat gctatttttt aatatcaaca
     1561 aacaccagct caggtcggtt gaacaccctc ggtatcgcga taagcgttga cggcttccac
     1621 agcccgcggc ttacaacatg ggcggtgtca ccgtgcatat aaacgcctgc cgtgtatttc
     1681 gggaatactc cctgaccggg agcaaaaacg ccgtcaataa gtccgggtat cctccactga
     1741 ccgccgtgag cgtgccccga cagaactata tcaaagttgc cctttaaata ttcgtctatc
     1801 tgttcgggga agtgagcaat aagcaccctt atacgcttgc tgtctttttc cgaagtcacg
     1861 gcatcaagct gatttacaag ctcgccgttt atttcatcat aggtgtgggc atcgtatatt
     1921 ccgcagagct caagctcatt atctttgata tcaataactg tgccgtttcc ttcaagcacg
     1981 ttaacaccaa aatcggacat ctgctgtttt atccttgcac tgtcacctct tgcattttcg
     2041 tggtttccga tagtgtagaa gnnnnnnnnn ntccgaaaca aatgcaatgc gtacaggcga
     2101 tgataccttc ccgcttttta tctcgtaggt tgttgtctgc agccgcatat cacaggcgac
     2161 aagtcctaaa gcggcaagag ccgctcctgc aacgcctgcg gcacaaccta tagctattct
     2221 tttctttgtc ttttttgtga gcgtaaaatc acctcttaat atccgcccga gccgtcaagg
     2281 ctggggtcat tttctatcca gctttcaaca ttgatgacct tgtattcgtt tgctgaggtg
     2341 cggataaata catcctttat tcctgcattt attataaggc gcttgcacat tgagcaaggc
     2401 tcaacctctc cgcagagcgt gccgcctgag gtcatatcgt gacaggcaag aaacagcgaa
     2461 gaacctatca tatcagtcct tgcggcgctt attatacagt ttgcctctgc gtgaaccgag
     2521 cggcaaagct cgtaacgctg acctttcgga acgttcagct tttcacgggt gcatatacca
     2581 aggtctgaac agtttttaag acctctcggc gcaccgttgt atcccgtggc gattatctca
     2641 tcgttgtgta ctattattgc accgaaattc ctgcgcaggc aggtgccgcg ctcgcatacc
     2701 gtttcggcta tgtcaaggta gtaatttatt ttatcgcgtc tttccatagt ttaacctttc
     2761 tgaaataggg ctgagcttac atcaagtcta atataacaca aataccgtct gtttgtcaat
     2821 taaaagtcgg gtgatgatat gaaaaaccgc cgtggaaaat cacggcggta aattttaatc
     2881 tgttttcttt gcatcgggga gcatatactt ctcactgaaa acagtgaact ttctgcggaa
     2941 ctcctcgttg tttgtagcct tgagatcgct tatatagctg tgtgtgtcaa agctgttgtc
     3001 gtgtatctgt atcatacaga cggcgatgtt ggagcagtgg tctgccactc tttcgtaatt
     3061 cgtgataagg tcggtcagca caaagcccat ttcaatggtg catttgcctt tacgcagacg
     3121 cttgacgtgt ctgtttctaa tctcggtacg cagattgtcg atcacctctt caagcggttc
     3181 taccgacttt gcctgctcaa tatctccatg ctcgaacgcc gtaatggcgg tatcgagtat
     3241 ttcgtttaaa gcaccgttga aaatgtgtat ttcctcaata gccttcttcg agaacgaaag
     3301 gcccttcgta tgcatttcct ttgcacattc actaatattg acagcgtgat ccgatatacg
     3361 ctcaaagtcg cttatgcagt gcaggagcat atttaccgtc tggctgtcgc cgggggtaag
     3421 gttcttggaa cccagctgta acaggtaact gccgagcttg tcctcataga catcgacttt
     3481 ttcctcgttc tcgtttacta tttcgacatc gcgatcgtta tatttggata taagccccat
     3541 tgagagcttc aatgtatctc ttgcacagtc tgccattctg acagctattg acttgcactg
     3601 ctccagagca actgagggag ttgcaaggaa acgggggtca agtatctgga actcgttttt
     3661 cggagcatta tcgtccttct tgtcacgcac tgagatatat gcgagctttt cgagcagctt
     3721 cataaacggc agccagatta tcgttgcgaa tatgttgaac acactgtgca ttatcgctat
     3781 gcctgcctgg tttgcagact gctcaaagaa ctcaaagggg aatatagcgt ttatgatata
     3841 gaacagtatc ataaatacta tagtgccgat gaggttgaag taaaggtgaa caagtgcggc
     3901 acgctttgcg ttcttgtttg cgccgatcgc ggaaagcaga gcggtgacgc atgtacctat
     3961 attctgtccc atgattatcg gcagggcggt gctgtaagta accgctcctg tagcgcacat
     4021 agcctgtagg ataccgaccg atgccgatga gctctgaata acggctgtaa gcacagcacc
     4081 tgcgacaacg ccaagcacgg gatttgagaa catagtgaga atgctcttga actgttcgtt
     4141 gtcagccagc ggcgcaacgg cggaactcat cgtttccata ccgaacataa gtatcgcaaa
     4201 acccataagg atagtaccta tatccttttt cttgctggat tttgacatca ttataaagaa
     4261 aatgccgatt attgccagta taggcgtgaa agatgtaggc tttagcagct gcataaatac
     4321 gctgtcaccc tgtattcctg tcagcgacaa cagccacgag gtcatcgtag tacctatatt
     4381 ggcacccata ataacaccga tagcctgcga cagcttcata atacccgagt ttacaaaacc
     4441 gactaccatt actgtggttg ccgatgatga ctggattacc gctgtgacta aagctccgag
     4501 catgatggct ttgaaagggt tggaggtcat tttttcgaga atttgctcaa ggcggcttcc
     4561 cgatagcttt tcaagtccct gtcccatggc gttcatgccg aacaggaaca tagcaagtcc
     4621 tccgaccata gtcagaatgc cgaaaatatc catagttttc ctttccagac ttgtcttctt
     4681 ttgatctgtt ttcttgccgt cggaaatgnn nnnnnnnnta acgttacatt tttgtatcgg
     4741 tgtatttacc gctttgttac agcttcttta ctcaaaacac gcagacttat ccccgtgaat
     4801 aaggcttccg tcttatacgg gggtccgctt tatataagat tatattactg ccaatgtgaa
     4861 tactaccgta ccgaaagtaa attctacagg tattgtaagc gtgaactttg attttatacc
     4921 gttcacggca tctgctgtag cggattcctg aggattaaga cctatttcct tgccggtcgt
     4981 ttcggaaata ttaacgttat aaagaccgtt gctgaggttt aagaattcgc tgatagcatc
     5041 ctttgcatag tccatatcgg ttatttcttc ttccgcaaaa cggcttgcaa atactgtggc
     5101 agtcttgttg tcggctgtta tggccgatac tgcgttgaaa tcgccgcaaa gctcctgtat
     5161 gnnnnnnnnn ngtctgtaac ggctgactgt aagggggtaa aatcatcgcc tacaaaacgt
     5221 ataaggttct tgaacagaac gtttacatac tccgagcaat aatccttgta ttcgccttct
     5281 atggcaaaga aagatgagat aacgttttct gttttttcat tatcctcatt gctgaagtcc
     5341 ttgtctgtaa gtccgttttc gctcttgtaa tcattaagta ccgtttcaaa ttcttcattc
     5401 gtaaggcaac ccatatctac gagcgcctgt ccgagtaaaa ggcaacctgt cttctgactt
     5461 gaaagaagct cttcaacctg agcctctgta agatagccca tatcaacggc aatatcgccg
     5521 atcctcttat caactgtcgc ctgcttctta tgtacggcat cgacctgttt tgccgtcata
     5581 tagcccgcat tgatggcaag tacgcccagc tttactcttg ttgtgtcctt cttttcgagg
     5641 gctacgcgca gattagcggc tgaaacgatc ttcctgttta aaagatagct accgaaaaac
     5701 tgtgtgaaca ttgcttaagc ctcctttttg aaatagttgt cgaggagctt gtcaagctgt
     5761 ttgacaatct cggaaatgtt gacgagaccg acggggtaac cacaagcgac gctcttatgg
     5821 ttcttcgtca acgggtttct gtaaaaagtc gtttgcaccg agttttatag cttcggagag
     5881 gtttccgacc gtacctaccg atgttgccat tattacaact gcatcggggg aatttgtttt
     5941 gataagctta agtgcttcaa ccccggaaag ttccggcatt acgatatcca taaatgtgat
     6001 atcgggaata atgtccgcaa acttttcaac tgcctgagca ccgtcattgg cttcgtaaat
     6061 ctgtgtgaag ccgtatgcag taagaagctt cttcatcttg ttcctgacca tcatggagtc
     6121 atcgcataca agtacttttg cttttgaaac atctttttcc ataattctgt tccttcctga
     6181 cctctgaaat ttatatcata ataatattta cccattacta agcgccgaac acaaacaggt
     6241 agctcagtgc aaaccattcc ctcacccagt agacagggat aagataccat ggtgttgcgg
     6301 cgctgacgca tttaacatca tatccgagac tttcggcttg cagtcttgcc cgccactggt
     6361 gaaagccgtc ggtaacaata gcgatcctta gcggcttgcc gttgtcttca agtatctgct
     6421 tggaaaacag cagattttca taagtgcttg atgatttgtc ctcttttgtg atacggcttt
     6481 gcgctatgcc gtcattcaca agtacctcat acatagcctc ggcttcgctg atatgctcgt
     6541 tatcgccctt gccgcccgaa acgatacaat cggctttttc gtnnnnnnnn nngactcgga
     6601 ccgtcacgtc tgacctgaca gccgagtacg ataaccgtgc gtgagccgtc cttttcgggt
     6661 gcgtcgttca tatttgcgac cataaaagcc gagagcgtta tcgcatatac cacaagcacg
     6721 ccatataaga tgagcacggc acggaagata tatcttaccg ctttttttac gttatttaac
     6781 agcttcgggg tgaatatacc gagaagcaca gttacgccgc ctatcacaaa cccgaaataa
     6841 gagcctgtgt taagcgttcc gctgaatagc ggtattatga acatcacaag cacagcaatg
     6901 ccgataacgg ctagcgta
//
LOCUS       DAWAOG010000034         6915 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1557_length_6915_cov_2.267201, whole genome shotgun sequence.
ACCESSION   DAWAOG010000034 DAWAOG010000000
VERSION     DAWAOG010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6915)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1557_length_6915_cov_2.267201"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1689..1698
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6642..6651
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1661 a         1828 c         1437 g         1969 t
ORIGIN      
        1 aaaatcatgc ttgttgttat acgccgagta cccctcgccg tactcctcat agatattgaa
       61 caagcctata gcaaatatct tccactcagc ctcttcatac agagtattga aggttatcgt
      121 ggggtgatcc ttgtagaact gcattgacgg cgcatcatag ctgtcataaa gcgttctcca
      181 gtattcgctc agacacgcaa agaacgtacc tacagccata ttgtgaccgt acagtacaag
      241 atttcccgag ttcccgtcgg gagaaaccac gcagttacag tccatcatta ccgagccgct
      301 cttgctgacc tcgcgataaa aatcgtgcgt cagataatag tcgttatccg agccctttac
      361 aacaggataa tctattgccg tgccctctat cctgagatat cccaccgtat cgggatttat
      421 ctcaaggagc ttcttgaacg acggcagaac ttccttgctg ctgccgttgt tgcctccgtc
      481 agggatatat atattcgata taccctgact tctccactgg tcgcggaaca tcagaaatat
      541 atcggaaacg agcggtacgg ctgttaccag aaaaacaata agcgcgaccg taaaaacgat
      601 ctttcttatt actacgccta cactgtcgcc tttccagggg ataatgccca tgatacgtct
      661 tttccaaaat ccgggctttt ttgcagccac gccgttacgg cgcggatact tctccatata
      721 tgttgtcatt attgctcctt agatcatcgg taacgctcag tcggttttat cggggaacga
      781 ataaccgtca cgctttgcgt cttcggctgt atagctgaga agctttcttc tgtcccatgt
      841 gctcataaac cagtcatagc cgttgcttac cttgtcatat acccactgcc aacgctttac
      901 ataggtattt acgaccgcct tgcttacatc aacgtccctg ctttcgccct cgcgtatctt
      961 tcttgcaaat accgcaagtc ttaccgtttt ctcactgccc tcatacggcc agcagcaggt
     1021 cgaaagcgtc agtatatcgt caccgtactt tatatcaaca tcggtgaaaa tatcgcttct
     1081 gtccattatg tttatgataa aatcgttgaa atcatcctcc gaggtgaagt cgtatttcag
     1141 attataacca tatacctcgc ctcttgactc ttcaacattg tatatgccaa agccaaatat
     1201 cttccattca gcctgctcat acagcgtatc gaacctgatt atcgggtgct ctttatagaa
     1261 ggacttactc ggctcgtcat acgaatcgta catggtgcgc cagtattcgt taagcggcgc
     1321 gaagaatgta cctaccgcca tattgtgacc gtagagtatc aaatttcccg agtgaccctg
     1381 cggagttacc ttgttgtggt aatccatcat aacagtgccg cttttgctct cattcttgta
     1441 gaaatcgtga gtgagataga agtcgttatc cgttcccttt acaacaggat aatcgacaag
     1501 cgtatcgttt atctttatat aaccgactgt gtccggattt atttcaagca gcttcttgaa
     1561 tgacggaaga acgtccatat tatcctccgg gacatcggtg tcatcaagct gatacatctg
     1621 tgatatacga ttacttacct gctggtcttt aagcatcgaa attacatcaa cgagtagagg
     1681 tatagctgnn nnnnnnnncc tttcgccatg cgataaaaaa tgttatgctt cttttcagcc
     1741 gccttgactt ctccgtcatt atccggttct tctgcgttct cttcatcatc ttcaaactct
     1801 gcggtgcttt cttcttcaaa agcctcctgc tcatccgatg cttcatccgc acccgaaagc
     1861 tcatccgata tgctttcaac atcttcagcg ccgtctgtat aagatgcctc ctcggacaag
     1921 acctctttat ccgacatatc gttttcgtct gtgccctctt caaggctctc ggttatcgta
     1981 tcttttctgt ctatatcggc gagtatctcg tctacggtct gtctatccgg tctttcgttg
     2041 aatttgttat cgttttccat gactgtctcc ttgcctgacc gggtttttac gcccgatcgt
     2101 gttgttcctg ttctgtctgt atctcttctg ccgcccatct gagtgcggca agcactgtgc
     2161 tttttctgat atattctctt gcattttcat cgccgctgag cgcaggatct gtatacacaa
     2221 gtcttgcctg cgtcctgtct tttgtactca gccctacata taccgttccg acaggtgtat
     2281 ccgctgtacc cccggtaggt cctgccactc ctgtgacaga tatgccaatg tcgcttcctg
     2341 cggtcttacg gacaccgtca gccatcgccc ttgcggtttc ggcgcttaca acgccgtatt
     2401 tttctatcgt ctgcggcggc acgcctataa acctttcctt ggcgcttacc gcataggtgc
     2461 agatactcat ctccataaca gacgatgagc cgctgacaga cgttacggcg gcgcttataa
     2521 gcccgcccgt acagctctcc gcgcaggcga ttttctcacc cttgccaact aataattgta
     2581 ctacattttc agcggttttg tcaataagcc gctgaagctc tccgatacgg ctcataaagc
     2641 cacatctcct tttttatcct ttgcggatta ttcctgttct tctttgcttt ctatcctgaa
     2701 cagctttacg gtagtaccct cgacagcctt ttcgataagc ggtgcaaagt cgaacgactg
     2761 ctcagccttt atcacctcat cgaataccgg acgtgtcttg gggtgcttcg gtgtgaatac
     2821 cgtacagcag tcctcataag ggagcgttga cgtttcaaac gtatctattt ttcttgctat
     2881 atctatgatt tccttcttgt ccataccgat aaccggacgg aatacgggat attctgcggc
     2941 ggcatcggta cactgtatag cctttaacgt ctggcttgcg acctgtccga cactttcacc
     3001 ggtgacaagc gcgccgtact cgtttctttc tgctattata ttagcgatct tcaccataag
     3061 tctgcgcatt atgatagtaa agaactcctc ggggcagttg tccctcagcg cctcctgtat
     3121 ctcggtgaac ggaacgcaat aaaatctgat atccccgcag tacggtgtca gcttctcaca
     3181 gagcgtttcg accttcataa gtgcacgctc agatgtgtac ggaggactta caaaatgtat
     3241 cccgtctact ataagtccgc gctttgccat catataaccc gcaaccgggc tgtcgatacc
     3301 gcccgacagc aaaagcagag ccttgcccga actgcctacc ggcataccgc ccgcgccgat
     3361 tatacgctcg gcgctgacat acgcaccctt gtccctgatc tcacacataa cagtaacctc
     3421 cgggttatga acatcgaccg acagatgagg atacgcttca agtatagccg caccaagctc
     3481 gacctgtatc tcaggagttt tcatcgggaa cgacttatcc gaacgctttg cgtcaacctt
     3541 gaatgtcttt gcgttcttca gggcattttc aagataaggg atactctgtg cctttatctg
     3601 ctcaaaatcc ttttcaaaca ccgcacagcg ctgtaatgca cctataccga atatgacctt
     3661 cagcttttca atgacctcgt caaggtcgca ttcgccgaca ggttctatgt agatagtgga
     3721 ctgtcttctc atgtactgga actttccgca gtggcgcagt cttcttctga tattcttcac
     3781 gagtatatcc tcaaaagtac ccttgttgag tcctttcagc gctatctcgc cgtattttgc
     3841 cattataagc tctttcatta tatgtttcct ttcctgcggc ttttctttgt acgtctgaag
     3901 cgtactatgc cgttttctat ttcattgcaa agcgtgtcta tatcttcttt tgtcgtttcc
     3961 atcgaaaagc ttacccttat tgtgctgtca gctctttctg cgttcattcc gaaagcgtcc
     4021 ggcacggagc ttatcttacc ctttgagcag gcagaaccgc tgctgacata tatctccttc
     4081 tcctcaagcg aatggagcat gatctcgctt cttaccccaa gcaccgagaa gctcagtata
     4141 ttgtcaacgc tgttgtccgg gctgtttata taaacttcct ccatccgcgc cagtctttca
     4201 cgcagatgtt cattcagctc cttataatgt gaaagatccg caggataagc ctttaccgcc
     4261 gcctcaaacg atgctataag ctcagtcggc tctgttcccg ggcgcagatt tttctgctgt
     4321 cctccaccat ataccagcgg cgtaaatcgc accttgtccg cgcagtaaag cgcacctatg
     4381 cccttaaatc cgtgtatctt gtgtcccgac aggctgataa ggtcgccttt cagcggcact
     4441 ttgcaaaacc cctgtacacc atctatatga acgatcgtgt cattgttcac gttttttaca
     4501 agcctgtaca gcttttcggt gtcgacctta aagccgttct cattatttac agccatgcac
     4561 gatacaagga tagtatccgc ggtaacgtta tcggcgatat gctgttcaaa gctgtctatc
     4621 gcgtccttag gtgacagacg tattatttca aagccctgct tttcgagata gtccatcgtt
     4681 ctcgtaactg acgggtgctc catagcagtg gtgactatcc tgtttcctct gcgtctatat
     4741 gcgtccgccg caccccttat cgcgaggttg ttgctttcgg tcgcgcctga ggtaaaatat
     4801 atttctccct gcgcgccgag ctttttcagc agtgtctttt tcgcgcttat cataagcttt
     4861 tccgactcta taccgagcct gtgcagtgac gatacattgc cgaaacagct tcgtgcggca
     4921 tcctcagccg cttttatcgc ctgctcacaa ggctttgtag tggcggcatt atccagatat
     4981 atcaaatttc tgtctccttt tcatatgggc tttgcccact tatctataag ctaaaggcta
     5041 cgccgcataa ttattgtcgt gcgatcgcgc agtcagattt ttcgtcaggt tgtataatga
     5101 ggacgacaca aggctgacgc cttgttagga cgaattgtgc aagatggcgg aaaatatgca
     5161 agcaagcgcg cgacaaaggc gttagccggt gattttgcgg cgttgcctta attatacacc
     5221 attttgcgat aaaaaaacag ggtatattct gaaattttta tgaaaaaata accttgtata
     5281 agcattaccg ccgattttat gaaaggaaga acatattatg tatatttcaa aacgctgttt
     5341 tgtgcggata atcagctttc ttaccgcagt cacagtcgca gcaggaattt ccgccgcgct
     5401 taatatgaac agctcagcgc agtataagcg cagctttgaa cagaatatga cccgaaatgt
     5461 cgaggacttg tccgcagaga tagacaatat caagaacacg ctttacaagg gtatgtatgc
     5521 aggcacaccg gagatgatga cacagctttc atctaagctc tggagcgatg cgtccaccgc
     5581 gaaatcctct ctcgcacagc ttcccgtagc cgggcttcat cttgaaaaca cctataaatt
     5641 cctctcgcag gtcggaaatt tctccaaaag ccttgcaaag cgttattccg agggcgaagc
     5701 actgagcgcg gacgacagaa aatcactgca aacgctcagc gaatatgcag atcgcctttc
     5761 cgaaaatatg tggaatgtcg aacagcgcat aacaaacggt gagttatcct ttgaaaaggc
     5821 aatagctgag gttcagggca cagaaaacaa cgaagaacca tcgtatatca ccgagggctt
     5881 taccgatttt gaagagggct atgacaacta tccgaccctt atctatgacg gacccttctc
     5941 cgaccacatt cttgaaaaag agcccgccat gctcaaaggc acaaccgttg taacgctctc
     6001 cgatgcactg aaaaaagccg aaaaggcttg cggcacaaca ggacttaccc acagcgatgc
     6061 cgatgacgag caaggcagaa tgccgtccta cgttttcaca aaggataatg ctaccgtgtc
     6121 ggtgacaaaa tacggcggat atctcagcta tatgatgcgc aacagagatg tacccacccg
     6181 ctcaatgacc gcactcgaag cagtaaacga gggcaaaaag taccttgaaa tgctcggtgt
     6241 caaccatatc acctacacct attacgagat aaacggaaat gtctgcacaa taaattttgc
     6301 ggcggtcgaa aacgatgtta caatgtacac cgaccttata aaagtcagcg tggcgatgga
     6361 taacggcgat atactcggct ttgacggcag aggatatatc accaaccaca caaagcgcga
     6421 tctgcccgag ccgaaaataa ccgccgagca ggcgcgtaaa aagatatcct ccgagcttac
     6481 cgtaaccgac acctcgcttg ctgtagtccc atcatcgggc acaaacgagc tttattgcta
     6541 tgaatttcac tgtacctctc ccgacggcag gcagatgctc gtatatatca acgccgatac
     6601 cggcaaggaa gaacagatac tgctgcttga aatcagcgaa annnnnnnnn nacaaaagac
     6661 tgcaaatctt aaatcggatt tgcagtcttt tttgtttatt ttcttgttat cagtttttgc
     6721 atagcaggta gcagaacgtt catatcgata ggtttcggaa catggtcgtt cattcctgcc
     6781 ttgattgcat tgtccttatc ctcggaaaac gcattggcag tgagcgctat tatcgggata
     6841 tcggcttttt cagggtcgtc tatcctgcgt atcttggttg cggcttcata accgttcatt
     6901 atcggcatct ggata
//
LOCUS       DAWAOG010000035         6900 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1563_length_6900_cov_1.867933, whole genome shotgun sequence.
ACCESSION   DAWAOG010000035 DAWAOG010000000
VERSION     DAWAOG010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6900)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1563_length_6900_cov_1.867933"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    404..413
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2032 a         1374 c         1721 g         1763 t
ORIGIN      
        1 gtacagagcg ttgatatatc gctccctgac cgcttgctcg actgtatgag caaatacggt
       61 atcacatcgg atttctttaa tcttgaaata accgaaaccg ccgctgtaga agccggcgag
      121 ctgcttgacc ttaatatgtc gcggctgaaa actatgggct gcggcttttc gatggacgat
      181 ttcggaacag gttattcaaa cctgtcgcag atggcaaata cgggatttga tatcataaag
      241 cttgataaaa gccttatctg gccttgcttt gataagaacg gcgaaaagcc acggacgatc
      301 cttgacagct gtatagcaat gataaacggt ctgggaattt cgatagtagc cgagggtgta
      361 gaaactaagg agcaggtgga tctgcttact tcaaagggcg ttgnnnnnnn nnngcctatg
      421 aatgaacatg attatttgaa ctttttgcaa aacaattgtt agcgtaattg ctcagccttt
      481 tgtttatgaa tactaaaata tttagattgc tttacaaact tgtcagcctg tgtgctttta
      541 caagttacat cgaacaaaaa cgtgagcttc attattatgt cgttaaaaat gttaataata
      601 ttatgaacag atttgtataa aaagcggcaa aactccctga attgttccgg aaattatttt
      661 tgaatgtgta tattttacga aaaagttaca aagtatattg acaattttat tttaatgtgt
      721 aataatatga agtaaagata aggaggtagt tatcttatga tgggaattaa agaaaacttt
      781 aaaagaggca cactcgagat gcttgttctt catctcttaa acggtgaaga aatgtacggc
      841 tatcagttga ctcaggaaat cggcaaaaga agcaacggca cattcagaat taccgaaggt
      901 tcaatgtacc ctacgcttta cagactctta gacaaagagc tcattgctga tcgtcttgaa
      961 cttgtcggaa gaagacgtac tcgtgtttat tatcacattg agcctaaggg tgttgaatat
     1021 cttgaaaagc tggaaaagga atatgaactt accagtgcag gtattgatgc cattatgagt
     1081 tatgttcctg aagacgaaga ataatcgttt tttcgttgca aacggcggtg tacgttaaga
     1141 ggtacaccgc cgttttatca catttacggc atattattaa ggagaatgat atgttaaatt
     1201 acagtaaata tataatagac gctcatgctc atatttttcc cgataaaatc gccgttaaag
     1261 cggctgacaa tatagggcga ttttatgatc tttatatgaa ctttgacggt acgacagata
     1321 cactgataaa gcagggcgat gaatgcggtg tttctaaatt tgtggtgcag tcggtcgcaa
     1381 ctgtaccgca tcaggtaaag cgaattaacg actttatcgt gtctgccgtt cagaaatatc
     1441 ccgacaggct gattggcttc ggctcgcttc acccggatat gaatggcatg gaagaggaga
     1501 tcgacaggat tatttcaatc ggacttcacg gaataaagct acacccggac tttcagaaat
     1561 tcgctataaa cgatgaaaat gcctgccgta tatatgatgc agtgggtgat aggcttccgt
     1621 ttcttattca tacgggcgat aagcgttata aatattcaaa tccctcgctt atggcagaga
     1681 tagcaaaaaa atatccgcac acccgcttta ttgccgcaca tttcggcggc tggtcggagt
     1741 gggaaaaggc ggagcaggag cttgtgggac ttgataatat atgggtagat accagtagct
     1801 cattctattc gatgactccg gaaaaagccg cagagcttat cacaaagttc ggcagtgaca
     1861 gggtgttctt cggcacggat tatcctatgt ggagagcgga caaggacctt gagtttctcg
     1921 acaaaattcc actcagcgag gatataaagg aaaaggtgct gtggaagaat ataaacagct
     1981 ttctcggatt ggggctttgc tgatgaagat atttcctctt atattcatat ccccgatcaa
     2041 gaaaaaagct gtaatcaaat cgggctatca cgattattcg caccgatgcg gaatgcttct
     2101 gctcgaaaaa gctttttcgt atatcggaaa accgttttcg gaaaacgaca tatcaaaaac
     2161 ttcaaacggc aagccgtatc tcagcgacaa ttcgctgtat ttcagcattt ctcattgtga
     2221 cggaatggcg gtgtgcgctt tatccgaaac acctgtcgga attgactgcg agagcatacg
     2281 gcgcaccggc acagccgtta tgaaacggtg ttacagcgca ggcgaaacgc agtatgttct
     2341 gacttcagca gacagcgata ctatgttttc gtgtctgtgg acattaaaag aatgctatgt
     2401 gaagatgacg ggtgaaggca tagcatcgga tttaaagacc aagtgctttg atatgaaaaa
     2461 cggaaggacg ctttttgaaa gcggtgcggt tttttatcat tatgaccgtc gctttaataa
     2521 tgaacattat tttgtaagcg tctgcattaa gacgattgat gaaaagttgg caaataccat
     2581 taaaaaggca gattttgaca ttgacaataa gcatattttg ttgtacaatg tatcagacaa
     2641 ctgaaaaaag cgaacgggag cttgtttgac aggctgagag ggaaatgata tttccgaccg
     2701 ataacttgat ttgggtaatg ccaacgtaag gatacatacg catacggtgg caatctgtat
     2761 gcgttttttt attgcccgaa aggagttagt atatgaatat aacagcaaag cagatgcttt
     2821 tgtatgctgt cagcgacagg gcatggagcg gcagcgacga ggaatttctg tcgcaggttg
     2881 aacaggcgat agacagcgga gtcacggtat ttcagcttcg cgagaagaat acagactatg
     2941 agcatttcaa ggatattgcc cttaagataa agccgatttg caaggctcac ggtgttccgc
     3001 ttattataaa tgacaacgtt aagcttgcaa aggaaattga tgctgacggc gtacatttag
     3061 gacagaatga ccttgacata aaagccgcaa gagaatatct cggtgcggat aaaataatcg
     3121 gagtttccgc tcacaacgta aaagaagcac aggcggcaga aagtggaggt gcagattatc
     3181 tgggaagcgg tgcggcattc gttacttcca caaagacgga cgcgggcgca attgatcaca
     3241 aggtgctgtc ggatgtagcc cattcggtca gaatacctgt agttgcgata ggcggtataa
     3301 ccggtggtaa tatatctcgc cttgagggac tcggacttga cggagtagcg gtcgtatcgg
     3361 cgatatttgc cgcaaaggat atcccctcgg cggtaaacga gcttaaggca aaggctgaaa
     3421 gagtagcgga aagctcgtct aaataagaaa ggatttgata atatgaaaaa gatacttaca
     3481 atagcaggct ctgactgtag cggcggcgca ggcatacagg ctgacataaa gacgataacc
     3541 atgcacggaa tgtacgctat gagcgcaata acagccctta cggcacagaa tacaacgggc
     3601 gtatacggca ttatgaacag ctccgctgat tttctgaaaa atcagataga ctgtattttt
     3661 aacgatatcc gtcccgatgc cgtaaaaata ggaatggtga gcgatgttga gataatcgag
     3721 gcgatcgctg aaaagctgaa agaatacaag gcggtaaata tagtagccga cccggtaatg
     3781 gtagctacca gcggaagccg ccttataagc gaggacgcta ccgatgcact tatgacaaag
     3841 ctcctccctc ttgcggatat catcacaccc aatcttgccg aggcagaggt gctcaccggt
     3901 atgaagatca caacaagaga agagatggag cgggcggcaa aggtgctttc cgataagctc
     3961 ccttcaacag tcggcatagt aatcaagggc ggacaccttg acgatacggc ggacgatctg
     4021 ctatatgtaa atggtacgaa ccactggata aacggcaagc gtatcgataa cccgaatacg
     4081 cacggcacag gctgtacatt ctcttctgcc atagcctgca accttgcgct gacttccgac
     4141 aaggtacagg ctgtcacaaa cgcaaaggaa tatcttgcgg gtgctatcgc cgcaaagctc
     4201 gatctcggtc acggcagcgg accgcttatg cacaactata agatagttga aaaggatata
     4261 taaaatgcct tttgtagagt ttaataatgt cacattcggc tataaggagg acgattatct
     4321 tcttcttgaa gatctgtcgt tttcggtcga aaagaatgat ataattaccg tgatcggtgc
     4381 ttccggttgc ggcaagagca cacttttccg ccttatgacc tgccttgaaa ccgcttacaa
     4441 gggcgagata catattaacg gcaggacacc gaaagaagca acaggcattg cggcattcat
     4501 gccgcagaag gatctgctta tgccgtggcg gaatatactt aagaatgtga ccttaccgct
     4561 tgaagcactc ccgatatcaa aatccgaacg tgtagctcag gcgagagagg cacttgaaaa
     4621 ggttgggttg gcaggttgtg aaagtaaacg tccgtccgag ctttccggcg gtatgcgtca
     4681 gcgtgtgtcg tttgcgagaa cgcttgtaca gctttggcac ggcagagagc ttatgctcct
     4741 tgacgagcct ttctcggcgc ttgattcgct gacaaggatc tcaatgcagg actggcttat
     4801 cgggcaggta aaggggctta acgccacagt tatgacagta acgcatgatg ttgaagaagc
     4861 gatgcttctc ggcaataaga tatttctgct cagcggcaga cctgtaaagc agataaaggt
     4921 gatcgatgta agtcatatcg gatgccgtga ggatctgtat acgccgtcat cggttgagct
     4981 taagaatgag cttgtgtcgg cgttccttac tgaaaaggca gagaggggag ataatatatg
     5041 aaaaaacatc ttcctgccgt actgacggga ataggactgc ttatattgtg gcagatgatc
     5101 gcaatgtgca taaacgcggc ttacattctg ccgtcaccga cacagatcgc ggtgaagata
     5161 tgggagcttc gtgacccgct gtttacggtg cacacccccg caacaatgct tgtaacgctt
     5221 atcggacttt tgatttcaat agttctcggt gtgggacttg ccattgtaat ggagcgtttt
     5281 gagtatgtcg aaagggcact atatcccgta atcgtcacaa cgcagacgat accggttacg
     5341 gcgatagctc cgctttttgt attgtggctc ggatacggta tatggagcaa ggtgcttgtt
     5401 gcggtgctta taacattctt cccgataact atatctgtac acgacggact taaatccgca
     5461 aaaagggaaa atgaggagct gctcaggact atgggaatgg gcagtacggc gatatttttc
     5521 aagctgaaat tccccgccgc attaccctca ttcttttcgg caataaaaat ggctataccg
     5581 ctcagtattc tgggagcggc aatgggagag tggctcggcg cacagagcgg actcggatat
     5641 ttcagcaagc gtatgatgac acagcttgac ggtgcgggag ttttcgcgcc tgtagtagtg
     5701 cttgctgtat gtgcaatgct ggttgtagct gttatcagtg caatagaaag aaaacttata
     5761 acatggagaa aggaaattta acatgaaaac caaaagattt gcggcatttg ccacagcggc
     5821 ggctattatg ctcggtgtgg caggctgtgc aggcaacacg acatcatcga cagaggaaag
     5881 cggcacggac gtttcttctg caactagcga aaccaaggag cttgaagagg tttcggtcgt
     5941 actcgactgg taccctaacg gctcacacgt ttttctgtat gacgctatag aagagggcta
     6001 ttatgcagag gagggactta aggtaaaggt tgagtttccc tcaaacacaa acgatgctat
     6061 ctcaatgact tccgcaggta aggctgatat cggcttttac tatatgcatg atgttatcca
     6121 ggcaaatgca aaccaggata tccccgttgt atctatcggt gcggtagtac aaaatcccgt
     6181 aaatgtgctt atgtcgctcg gtgataagaa cataaagaca gttgccgact taaagggaaa
     6241 gaagataggc tatggcggtt cggttctcaa cgaagctttt gtaaagacaa tgcttaagaa
     6301 cgcaggtctt aaggaggacg atgcagagct tattgatgtc ggatttgagc ttatgtcgtc
     6361 aatgacaaca ggacaggtcg acgctactat cggcggattt gtcagccacg aagtacccga
     6421 gcttgaaaat cagggcttca cggtaaacta tatcaagcct acagacaacg gcgtgcccga
     6481 ttataccgaa cttgtattcg taacatcgaa ggacaatgca gagaagaatg ccgacaagct
     6541 gacacgtttc ctccgtgcaa caaagaaggg ctatgaggac gtaaaggcta atcccgaaaa
     6601 gggcgttgaa aatctgctca agaatcagaa caccgaaaac ttcccgctca acaaggacgt
     6661 tgaaacaaag agtgtagcta ctatcctttc tcttgctgaa aaggacggtg cacctttctt
     6721 atcacagagc gaggaaacat ggacaaacaa catcaagtgg atgcttgaca caggaattat
     6781 cacaaagtcg gttgacgcta aggatatgat cgtaaagctg gttgactgat atatattaag
     6841 actgagccgt ttgcttgatg caggcggctt ttgttatata tgacaaaaat atatttattg
//
LOCUS       DAWAOG010000036         6760 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1591_length_6760_cov_2.036838, whole genome shotgun sequence.
ACCESSION   DAWAOG010000036 DAWAOG010000000
VERSION     DAWAOG010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1591_length_6760_cov_2.036838"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    503..512
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2506..2566
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4500..4509
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1775 a         1535 c         1347 g         2022 t
ORIGIN      
        1 ggattgagtt tttccgaatc gatccatatg ccctgaccca taaggaacgg ataattcttt
       61 actctcttct gtgactgaat cttaaatctg tagaggagct gatctataac aaggttcatt
      121 ctgtcctcaa gcatttcaaa gaactttacg atatccccgc ctgcaagtat acccagtctt
      181 ggcaggttga tagatgtgaa tgaaaggttt cctctgcccg tcactatctc tcttgtagga
      241 tcataaacat tggcaataac acgggtacgg caacccatat atgctatttc cgtgttataa
      301 tctccgggct tatagtactg gagattgaaa ggcgcatcta taaatgagaa gttcgggaac
      361 aacctctttg cagatactct gcacgcaagc ttgaacaggt catagttagg atcaccctca
      421 ttatagttca gaccgtcctt gaccttgaag atatgtatcg ggaaaatagg cgtttcaccg
      481 ttacccagac ctgcctctgt agnnnnnnnn nnacattctt tattaccatt cttccctcgg
      541 gagatgtatc agtaccgtag tttatagacg agaacggtat ctgagcaccc gcacggctgt
      601 tcatggtgtt aaggttgtgt acaagagcct ccatagcctg atatgtggct ctgtctgttt
      661 caacttcagc ctgttttaac gcaaacttct gtatcttttc tattgtttca tctgatacat
      721 cgggacaaat ctctttaagg catatacgct ctgcttcttg atagtcattg tcgttagcga
      781 gcaccggcgc aagctgcctt tcttcctcaa gcttcttagt gtaagccttt acatcggctt
      841 ctgctgttgc aaggtcttca ataccgccga taagttcaag tcctcttacg atattctgag
      901 cataacggtg cttgtatgtc tttcgtacac cgtccgccat tgaatagtca aagttaggta
      961 tagactgacc gccgtgctga tcattctggt tagactggat agcaatgcag gcaagcgccg
     1021 agtagctggt tatctcgttg ggctctctta aaaagccgtg accggttgaa aagccgttct
     1081 tgaacagctt tatcagatca atctgacagc aggtcgtagt cagcgtcaga aaatcaagat
     1141 catggatatg gatatcgccg tccctgtgcg cctttgaatg ctcgggagca agcacataca
     1201 tctcgttgaa ctgctttgcg ccctctgaac cgtacttaag cattgtgccc attgcggtat
     1261 cgccgtctat attagcattc tcacgcttga tatcacaatc ccttgcagcc ttaaatgtaa
     1321 gatcctcata gatcttcata aggctggtat tcatttctct tacccttgtt ctgtcggctc
     1381 tgtaaagaat atacgccttt gctgtctttg cgtgaccgct ctcgacaagc accttttcta
     1441 ctgcgtcctg tacttcttct accgtaggca gttctcctgt tttgttggcg tttgtttcaa
     1501 gataattgca aactttttct gccatttcac ttgcttcctg atagtcattg ccgccgacag
     1561 ctctcgctgc tttatatata gcatcagcta tcttctcaat gttgaacggc actgtgcggc
     1621 cgtccctctt ctggatttta gtcagcattt gtttctccct ctcgtcgaaa attccttgtc
     1681 gcaccacaat atgtagtggt gagcgaaata cagttaatat tatatagtgt cttgctcggt
     1741 ttgtcaatat gtcttttaag aataagtgta taaaaatccg actgtatttt tagacaattt
     1801 gtcaattgac tgtatgtaaa tgccgatgaa aacacgattt ataaaatata aaatactgcc
     1861 gagttgcata gcaaaacggc agtacaaaaa atcatccgat attaaaaact catatagaaa
     1921 ttaagcaata taagtccaag taccacattg aaaccgatgc cgacgcaaat atatggtatt
     1981 ctcatacggt tagttcgcag aaagcccgat gcgatgccta tccatacaca tgatgccata
     2041 actgcgagca gaagtatcat aaccgctaca ttcttcggta gttcatatcc taaaaaactt
     2101 gtacatataa agcttgacac gcaatttaca agtggcagta ttccgagcgg aattgtcgca
     2161 agcacccagc gtatcctgtc tgctcttgca aacgagaaca ctatagcgcc aagtataagg
     2221 gtaattacaa gacactctat agccataatg ctctcctttc aaaaatatta cttagctatc
     2281 gagttgaagt cttcaatagc ctttgcgtta tcggcacata tgataagtgc aacgtagttt
     2341 ccgcttgtta caacattatt tccgtctatc ttatacatct catcaggcgt ataatcttta
     2401 aatgttgctg tctgtgtttc aacacgcttt tcgagcatag ccttcacatc ggcaacatcg
     2461 tcagccgact ttaactggaa taccggaatt tcatcaggat aagcannnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncccg acatacgatc
     2581 ttcacctata ggtgccatac cggggaactt tacatcagcc tgtatcttat cagtgatctc
     2641 ggttgccttg ccggaagcct gttctgtgtc ggaagaagcg gcgctttctg aagtacttgt
     2701 aactccggat gatgctgcgc tatccgaagc ggacgactga gcgttaccgc ccgagcaacc
     2761 gcatatcgaa actgcaatca ttgtgcagat aattcccgcc attattcttt tagtaaagct
     2821 gttcattgtt cgtaattcct ttcttttatg cacgttatat taagattgtt tctctaagaa
     2881 actaagcata acctgatatg tcgaacccat aaaatgaata ccgtccattt cgtgatattc
     2941 ttcataataa tatccgtcag gagctgtcag cacagagtgt aggtcaagaa attttgcctt
     3001 tgtatcggag ataacattcc gcaacgcgtt attatattcg tctatcgccg ctgtcgtcag
     3061 agcattgtcg taatccgctt cggcataata ggtaacaggc ggtatagaca acacatacac
     3121 tttagcttca ctgacctttg aaatatgcct tacaagttct cccatacagc cggcaaggta
     3181 atcaccgtcc gaatatccga cactgttcgt accgagcatt atatatattt tttggggttt
     3241 atttgtcttg atcttactgt cgatcgtttc cccgtcaatt tcgctttcaa gcgctgttga
     3301 cggcgcaaga ccgagctttg cgtatacaag cgacggatca agatatccgt aaagataaag
     3361 tcctgttgta atgctgtccc ctatgaacaa tgcatcatca aagaacgatg tgtcaaaatc
     3421 tgttgaagcc gccggagctt ctgcctgcga tggctgctga gtgttactgc cttttgtatc
     3481 ctgctgttcc ggttcttttg cggtttgttc aggctctgtg ccggactcag actcagtctg
     3541 cggctgtgtc tgcggttcgg cagtcgtagt ttccgttgtt gtggcaactg tggtttctac
     3601 tgttacagcg gtagtgaacg ttgttgttgc cgcagctgtc gtttgggcag ttgtaacgga
     3661 agtcgttggc ttggttgtgg ttgtcgtaat actctgatcc gcagatgata tgttatcact
     3721 gtctgaggaa gacgttacat cggtacagcc gaaaagaata accgaaagca cacaacccag
     3781 cgacaataaa actgatttgc ctttatttgc cttactgtgt ttattttcag tgctgatact
     3841 atcagttcct ttcatagcag cattaagacg aatgcttctg caaatagctc aacatcactt
     3901 catatgtcgt acccataaag tgaataccgt ccatctcggc ataatccgca tcaaaatatc
     3961 catccgaatt tgatatcaca taaatctttg cctttgtaag acctgatatc ttctcaacca
     4021 gctcattcat acttgccgca agagtttcgt tatcggcata tccgacactg ttcgtgccga
     4081 gcataacgaa tatcttctta ggcttgaatg cagcgatctt atccgatagc gtaactccgt
     4141 ctatctcagc gtcaagtgct gtagaaggtg caagaccctg ctgagcaaaa acgttatcct
     4201 gatcaaggaa accataaaga ctaagacctg tcgagatact gtcaccgata aacagcgatg
     4261 atgagaaata ctccttatca aaatctattg taggttgatt atccgttgat gtatcagaag
     4321 ccgtgccgga tgactcttcg acagaagccg tgctttcggg ggcagagctt acatctgaat
     4381 ctgtagcggg agcttcagtt tcaggtggtt cggatgtaac tgctgtgaaa ggcggttctg
     4441 atgatgtgcc gtcttctccg ttaattatat tataggtaaa gaaacacact gtgcatatgn
     4501 nnnnnnnnng ttcataaata tcattgacgg tgttatcccg aacttatttt ttcctttttt
     4561 cttttgtgcc aaaacacaca cctcgcaatc atactgtatt attatacagc attttaagga
     4621 aataatcaat agataaaaac aaaaaaatcg atgcctgtta agcatcgatt ttttaagctg
     4681 tttataactt agtcagaaac ataaggaata agagctacct gtctagcctt cttgatagca
     4741 gttgtaagct ctctctggtg gtatgcgcag cagcctgtta ctctgcgggg cagaattttg
     4801 taacgctctg tcatgcactt cttgagcttt gctacatcct tataatcaat gaattcagct
     4861 ctgtcggcac agaatgcgca aacctttttt cttgtgcgct tcataggacg aactgttgac
     4921 ttttcattct tttctctttc agccattgta ttatcctcct tattttgatc agaacgggta
     4981 atcgtcatca tcggcagatt ctgtaaaatc tgcattgtta ccctgcgaca ctgtgggcgc
     5041 ggtgtttgtg ttttgtgcag gatgcgcagg tgctgaagcg acctgtgcgt ttccgggata
     5101 gctctggtaa ggcgatgaag cctgtccgct gttagccttt tcacccgtga acgtggaacg
     5161 atcaactatg atctcggtaa tatatgcggt atttccgttt ttatcggtat aagatctgtt
     5221 ctgaagctca ccttcgataa gtatcatacg acccttggaa aaatatcttg atataaattc
     5281 cgcttcgttg cgccaagcaa ctacattgaa gaaatccgac tttctctctt cgccctttgt
     5341 ctggaatctg cggtctactg ctattctgaa agaaagcacc gacgcgcccg aagtagtgga
     5401 tctcagttcg aggtcattga ctatacgacc catcataatt accttgttat acatatgtta
     5461 ccgctcctcc tgcatacttc tccaaacaaa ttacttgcgt accgttacga gcgaacggat
     5521 aacgccgtca gtgatattga tgatacgctc gaattccata gggaaatcgg gcttgcagtc
     5581 gaagctgtaa actacataat aaccgtcggt ttcgtagttt atctcataag cgagcttacg
     5641 agcgccccat tcatcaacgt caactaatgt gccgtgttcc ttgatcagat cagagaactt
     5701 agcaacgaga gcctttactg catcctcacc gttcttaagt gaaaatacaa caactgcctc
     5761 atacttttcg ctgagctttg ccattataag cacctccttg tggattttaa gcccgcactc
     5821 ttgtacgagc aagggatgtt gacaaagttg tatacttgtc aagctctgtt attatatcaa
     5881 acttcgatct gtttgtcaag agttttttta aattcttccg aatatcagca taaatatcac
     5941 catggctatc ggctgatgga gcacatatat ccacagcgta tagcgtcctg ccagcgccag
     6001 cggcttgcag tgtgtcttat aaaaaaacgc cggcatctta tcttcaatag catactttcc
     6061 tgtataagca ccggcaagaa aaacgaagaa ccacggcaga agcgggaaat aatccatcga
     6121 ctgaaagctt gccgacctga agccgagcgg aaaaagaagc cccgcattat aaatgaactc
     6181 cgggagcttt attccgaccc ctgcggcagg tcctatataa ccgtacatta ttcctcttgt
     6241 aagaataaaa agaacggcac agatcgcaat tccgacggga gcggagattt taagaaaata
     6301 tttatctcct atcgcataaa tcatcatcga cacacccata aagtgcagta tgccgaagag
     6361 tatcggcgca gtaggcgcga agaatgcaaa tataaaggtg attatcatac cgatgaagaa
     6421 gcactgtata ccgcgttttg cgttatttct cgaataacgg cagctcatac cggatatgaa
     6481 catgaacgca ccggcaaaag catcacgaac aatatcaaac cagccgtcaa acattatcgg
     6541 tatgtgtata ttatatgtgt aattcaaatc gaaacaaatg tgataaacca ccatacagag
     6601 tatgcagaag cctcttactt catccataaa gcctgcgcgt ctgtattttt ttacctgtgt
     6661 atttgatgat gtagctgtat tttccattta acactctctt ttttcttttt acgatttagt
     6721 cggacagaat tattatccca taaatcaact cactgtaagc
//
LOCUS       DAWAOG010000037         6677 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1608_length_6677_cov_2.088040, whole genome shotgun sequence.
ACCESSION   DAWAOG010000037 DAWAOG010000000
VERSION     DAWAOG010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6677)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1608_length_6677_cov_2.088040"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    864..873
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2146..2155
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3019..3028
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5922..5931
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1487 a         1682 c         1397 g         2071 t
ORIGIN      
        1 gtgtaaagtg atgccatatt gctctcggca tcatacagag caagattata tttattcatc
       61 gattccagct tttcgttagt ataagctaca tcaaaatcac agcagccgca actttgcccc
      121 tgtatcattg cgaaatggac acgcttatcc gacggctgct catcaccctt aagatatccg
      181 catgccgtgt cggcaacggt agcggcaagc tctttcggat ccatctgtgc ggtagttaat
      241 ctggtggtgg aataaatgcg ctcctggtat atagcgtcaa accctgtgac gataatatca
      301 tcgggaacct tcattccgtg ttcttcaagc gttttcatag tcgttattgc catagtgtca
      361 ttggcacata taattgcctg aggcattttt ctgccgctgt taataagccc ggaaacgaca
      421 ttttttgtcg gcgtatccca aaaatcacca tagccgagca tggtattttc attaaaatcg
      481 atcttgtggc gggcaagtac gcgcttgaat gcttcaattc tgtcttctga gaaccggttg
      541 ccttttgttc ctgcaataaa aaacaggtcg gtgcagttat gctcggttat aacgtgttct
      601 gttatctgtt caagtgagtg tgcatagtca tatattatat ttgtacagcc gttatgtctg
      661 ccgtcgagta taatcacagg tgtttttgtt gcgtgtgcat atcttatgat agtatcagtc
      721 acccattcgc tttttattgt ttcaggtata atgacaagaa catctataat atcaaagtta
      781 agcaggtggt atatgcttgc ttcgcctttt gaatacggtg tatcgtaata catattcgta
      841 aaagcgttga atatcatgac cttnnnnnnn nnncttgaag cctttttcca ccaaagcgtc
      901 ggaggttatt tttgcaatat gggctgtatg gtcatcctgc attcctacaa ggcatattcc
      961 ggctactgcg tatttgtgaa attttcctgt gtacaagccg ctcacctctt tttttcggtt
     1021 aatcaaatca atgtaatctt gtttaattat accataaaat agaattaaag tcaagataat
     1081 agtgaatgtt gcacaaaaac aggggctgtg acacaacagc ccctgtttga taataccatc
     1141 aatattttct tacgctctgt accgccgcca taaccagtgc cgccgcagtg ttgtaaagtg
     1201 ttattacgct gggtgtcagc aatgacatat gccttaacag caccattata agtacgagca
     1261 gaagtccgag cgcagatgtg ccaagcataa ccgcttttga aaccgagaag tcgcgcacaa
     1321 ggctttttgc tgccataacg cctcttgcaa aagatgtgaa tgtgccgcag caggagagcc
     1381 cgccgcttcc gcgtcatgta tactttgtgc tttcgctgta ttcgtcgtgt gcttcgtggg
     1441 gaagtacttt tataagtgaa gcgtcaagct caaacagatc tgcaaggttc tccaccgtta
     1501 ccagtgagtc tgtagtatgc acaagcaccg ttatcccctt tgactgaagc tcgctcagag
     1561 tagcctgtat ttccggatta gccgtcattc ccactacaaa catcgccacg atctcaccgc
     1621 tcaccgcaag ataaaccggc tcaagtccct ccgggcaata cttgcgttcg ttcttcttgt
     1681 tcggaaggtc tatgttgtgc atttccataa gcgccctgcc accgagcata acacgctttg
     1741 ttcctatcca gccggtaacg ccgcagttat cttcatatat gcagttgtcg accttcttta
     1801 aaaggtcctt gttgccgagc gtcatatcat agaatggata tgagagtatt ccgtccgtat
     1861 gtatcgcaag gcttgccgcc ataagtatcg cttcatcaac gcttgtttta acaactgagt
     1921 ttttcttctg ccagcgcttg agcttcttta cctgtacgct tcctgcgggg aagagcgcct
     1981 ttgcatcgac cataacggtg tttacatcgg aaaattggac tgccgcatca tatccgagga
     2041 gagcggcgtt gcactcgctg agtttctttg aagcgcgcgc aagcggtctg ttcacgacaa
     2101 gcagaagcga gaagggtgca accaccgaca cagttgccac tgccgnnnnn nnnnnccagt
     2161 atatcggatt ttcagcaccg gttgtcggga actcgctgcc gaacggattt aaaaacgcga
     2221 gtattcccgc tacaagtcct gccgccacag atgccactat aagcttcacc gacattctgt
     2281 cagcctcatc atcgcagtag cttgatttaa gaaagtccgt cagaaattca gtcttgcgca
     2341 ttgccgcaat tactggaaga cggtcaccga ctgctcttgt cagtgccgaa acgtgttctt
     2401 cgccgtcgat aacctcggcg taatatttct gactgtcacc tgatacaaac ttgaagttcc
     2461 gctttgcacg ggatatcata tatatttttc ctattgtgtt gaacataagc ccgacaagtg
     2521 ccgttgaaag atatataaac gaatgtccga gcttgaaggt ctgcacatcg accaatgcga
     2581 taactccgcc tataagagca agcgccgatg tcagtgtgca taccgagtcg cagtccgctt
     2641 ttcccgtaaa cagctttaca gcgccgttgc tgatagcggt cgagcatatt gcaaagccga
     2701 gtactcccag aacaagatat atatatataa gtgagtttac atctccggtc gtaccgttta
     2761 taaagttgaa gcttgcaccg agcccgagct ttgcaaacag gttgaaatcg ttgagtgcgc
     2821 tgataattac gcttacagct gtaatgacgg tcagtagtcc gagccttatt ttaagtccct
     2881 tgtggcttgc acacagatcc gcccatatct gtcctgtgct gtcatagtta tcaaactctt
     2941 cttcctgctc gggctcttct tcacgctcgg gtatctcgtc ttcaagtacg aaatccttta
     3001 gcttgtgctt gttcgccgnn nnnnnnnngt tcctgagcct tttgataggc ttccgcattt
     3061 gccgccgcaa gttcagcctg cttttcttct tcgatcgcag gattttctgc ggggagactg
     3121 ccggtagctt ctattatctg cttctgatag tcgatgttca gcgggtgggg aagtgtctgt
     3181 ctgtcgccgt ttatcgtgtt cagtgttatt gtacggtgag ctctgacatt ttcctttctc
     3241 tgttgagcaa ggtttttgtt gagcttttct ataaggttgt cggtagtttt cttgccgtga
     3301 tattccttta ctgtttcttc gccgctgtct tcgttatcag tcgtatgctc atcggcgatt
     3361 tttatgtccg cggtttcact tatcttttcg gcaatagcgg ttttctgcgg agctatcgcc
     3421 ctgaggtcgc cgcctgctat gccctgagtg tttcctgcgt acatattgct cagttcggct
     3481 accgacacaa gctccttttc cctgacctgc tcgtttacct tttcttttac ttcaagaggg
     3541 tttacaagcg ccagcatatc atcggggttt gtgttttcac gttccttttc gagcatcatt
     3601 ctgcgttttt cttcatcgct tacgatgtct ttttttatct catgcacagg ctctgcctgc
     3661 tttaccggtt tagccggaac ggggttttca gctacagccg cagcttttgc gttagccgtg
     3721 cgttcatcga tatctatctc actgctctca tcggatacaa caaagttgtc cgtttcgctg
     3781 agcaccttgt cgacatcgct cttttcgccc gttatttcat cgatatcaac atctgctgtc
     3841 ggagcgactt cgggctcggt atcgcgtgtt tccggctcgt ttatatactc tgcgtctacc
     3901 ttttcggtgt cgccgtcctc ttcacggata tatactccgt catcgctgcc gtcaagccct
     3961 tcaagtatcg ctgtagcgct tatatcgttg tgctttatcg ggttatcatc ggttttccgg
     4021 tcggcagaag aatcgctctt tttcgcgtca agctccgcta aaatatcgtc aattgaataa
     4081 tcagccatat caagcgtcct ttcttatatt tatttgcagg atcatctgtg ttttacgacc
     4141 tcatacatca atatgcctgc gcatactgac gcgttgagtg aatttacctt tcctaacatc
     4201 ggcagggata atacaacatc gcagttttct cttacgagct ttccgagacc gaagccctca
     4261 ctgcctatca caagagcgac cgcgcctgaa agatctgcct tatctaccgg cgttccgtca
     4321 gcttctgcac cgtataccca tacaccgtgc tttttcagcg tctttatggt gtctacaagg
     4381 tttgataccc ttgctatctt tacccacgcg gcagcccccg cagaggtttt gaacaccgtg
     4441 ggatttaccg ttgcactgcg ccttttcggg ataattacac cgtcagcgcc cgaagcctcg
     4501 gcggtacgga ttatagcgcc gagattatgc ggatcctgta tctcatcgca tattatgatg
     4561 aaaggcggct tgcccttact ttcggcgttt gcaagtatat cctccaccgt gcagtacgcc
     4621 acagcggata aagccgccgc aacgccgccg tgcttatcgc cgcacatgga cttaagcttg
     4681 tcttcgccga catcttttat aactatacct tgcttttttg caagagctat tatcttgttc
     4741 atgctctgtg agtttttaag cagatagact gtgtctatcg atctttcggc tttgagagcc
     4801 tccgtcacag cgtttttgcc gtatataatt tcctcttttg aagctgtttc cattatctta
     4861 cctctcgtat ctgcatacag atatgcagat taagtatacc acaagccccc tgccattgca
     4921 agcaaaacaa ggcccagccg ttatctttca caattttttc acttatgcgg gtgaatgatt
     4981 ttttacaccg taactgaggg aatgttgccg ccaacatcgc tattgacacg atcattgcca
     5041 aattttccct gcctgctcat tgcacctttg tccgcacatt gtacctttgc gatatgcacg
     5101 ttcacgccta tgcaaatcct atacctagcc cccaaacctt ttgccgataa agcgcggaga
     5161 ataccatata ggaaaaagca gtccgctgta cattacaacg gactgccatt tattgaagca
     5221 acagaaaatc tgctatcttt tgtgatttta cgtttaaaaa ttattacctt atttgcctga
     5281 ctatcaataa tgcggtatcg gttcaatacc tgttcccagt ttaggaggct ctttactttc
     5341 gacaacatat tcattgtaga tatagtagtt gcctgaggtt atgtaatagc cgttgtccgt
     5401 ttcaccgata atcgtcaacg gatcagcaaa ccaatattgt acctgaattt cgccgtacat
     5461 cggtatcgga tattttctgg cacgtaatat ttccgacttt gtccaaactt cttttggttt
     5521 atcaaagtat gattcattcc agacttcgtc catatgcgct gcaacgaatg tgtctatctt
     5581 ctccccccta ggcgtatact taacctcatc aacgcaatcc ttaccctcgg ttttaaagct
     5641 cttatagctc cagtttccgt aaactgtggt ttttcctata ttggcatatt tccttatgcg
     5701 gaacttatat gtagttgacg gcgaaaggtc gtttatcctc acgcgatcct tgctcttggc
     5761 aagctttgcg acctgcaccc acttgccgcc tttgtactgc tgtacaatat atcccgcagc
     5821 atcattactc atatcccagc cgaggttaag cattttgcct gtcttggagt tgagcctgaa
     5881 acggctgaca ttagtcggga tagtgttcgc gctcagcgtt gnnnnnnnnn naagcgccat
     5941 aaagcgcggt cttaccgctc atcttgtacg cctttaccct gaacttgtac gcagtgcaag
     6001 ccttaagacc tgcttttctg taagttgtag tgctgctgtt agctatctta gcaactctta
     6061 cccacttgcc gcccttgtac atctcaacaa tgtaaccatc ggcagaagca ttcttgctcc
     6121 agttgatacg gagcgcgtct gcggctctgc cgccgagctt agcacccttt ataacagacg
     6181 gatttgttct agccgtaaca gtcgcgctgt agttgccata aagtgccgtg ctaccgctca
     6241 tcttataagc tcttaccctg aacttataaa cagtcgaagc cttaagtcct gcttttctga
     6301 acgttgtggt gttaccgttt gttatctttg caactcttac ccacttgtta ccctgataca
     6361 tttcaacaat gtaaccatcg gcagaagcgt tcttgttcca gttaattctc agcgcgtctg
     6421 cggctctgcc ggaaagcttt actccgctta cctttgcaag cgtgggcttg tcaagccatt
     6481 catagtcaaa accgttgtct ttagcgtact gttctccggc agtgccgctg tagcagtata
     6541 ttttgaggtt gcttatcttc gtgtagccgt tatcataata gtagccgaaa gccttattgc
     6601 ctatcttcga cacactatca ggtatcgtga tactttcaag ctttgcacac cggctgaacg
     6661 caccgctgcc tattttt
//
LOCUS       DAWAOG010000038         6647 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1613_length_6647_cov_2.279126, whole genome shotgun sequence.
ACCESSION   DAWAOG010000038 DAWAOG010000000
VERSION     DAWAOG010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1613_length_6647_cov_2.279126"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1189..1198
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1566..1575
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6152..6161
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1971 a         1450 c         1510 g         1686 t
ORIGIN      
        1 atcctgccaa ggagatcgcc gaagcctgca agaacgcaaa gaatgttctg tgtgttgaga
       61 tgtcgatggg tcagatggta gacgatgtaa agatcgctct caactgctcg aagcccgttg
      121 agttctacgg cagaacaggc ggcgttatcc cgaaacccgc tgagatactt gaaaagttaa
      181 gagaaatggg aggtaagaac taatgcccga atttaaaaga cctcacgctc tggcagacgt
      241 gaccacccac tattgccccg gctgtactca cggtatagta cacagacttg tagccgaagt
      301 aatcgatgaa atgggcatcg agggcgatac tatcggcgta tgccctgtcg gctgctctgt
      361 tatggcttat gattattttg aatgcgatat gcttgaggct tcgcacggac gtgctcctgc
      421 ggtagctaca ggcatcaaga gagctaaccc cgataagttc gtatttacat atcagggcga
      481 cggcgacctt gcagctatcg gtactgccga gatcgttcac gccgctacaa gaggcgaaaa
      541 tatcaccgtt atattcatca acaacacgac ctatggtatg acgggcggtc agatggcacc
      601 taccacgctc cccggtcaga tatcacagac taccccctac ggcagaaacc ccgatgttga
      661 gggcttcccc gtaagagtat gcgaaatgct gtcgacctta agcggcgttg cgcttgcaga
      721 gcgtgtgacc gttatcgacc ccaagaatat aaatattgcc aaaaaggcta tcagaaaggg
      781 ctttgagttc cagaagaaca agcagggctt ctctataatc gaggttctct cgacctgtcc
      841 tacaaactgg ggcaagacac ctatcgaggc tcttgacaga gttcgtacag agatgatacc
      901 ttactatcct ttaggcgtat tcaaggaggg tgcaataaga tgacaaacga aattctgctt
      961 gcaggcttcg gcggacaggg tatactgttt atgggcaaaa tgctcgcata cttcggactt
     1021 tatgagaaca aggaggtttc gtggcttccg tcctatggtc ctgaaatgcg cggcggcacc
     1081 tgcaactgct cagtatgtat aagtgatgac ccgataggct cgccccttgt tgatacgccg
     1141 aatacgctta tcgttatgaa tacaccgagc tatgacaagt tcataaacnn nnnnnnnncc
     1201 gttgtacacg gcggtacggt tcttatcgac agcacacttg ttgacgcaaa gtgcgagagg
     1261 accgacatca atgtttatta tgttcccgca acggctcttg ctaacgatca tgatatgaag
     1321 ggtctggcaa atattatcct tgtaggaaag ctgttgaaag aaacaaatat cacatcgatg
     1381 gaaacggtaa agaaggctat ggcaaaggtt atcccgccta aaaaagcaca tcttatcgat
     1441 gccaatatca aggctattga gcttggtatg gcgcagtaat gccgactcag cggtatattg
     1501 cgaatttaat aaaacgaccc cttgtcgcag aattacctgc ggcaagggga tttgtgttat
     1561 tatatnnnnn nnnnngcttt accagcgaag cagataaatt tatcagcagt tgtttatcat
     1621 cgctactcaa ctttctgtag tttgagatta tctctctttc gtcggaagtc agactgtcag
     1681 tgttctcgat cccgaacaaa tagtccacag atacatcaag cttttttgca atctttttaa
     1741 caagttcaaa atcaggctgt cgcgtattct ttatatatcc gttaagagta gtaggagcaa
     1801 tattgagctg ccccgcaaac tccctttgtg aaatttctct gtcctcaagc actcttttaa
     1861 ggcaatcgcc aaaattcatg tcatcaccat tcctctctta tagaataccc gatatccatg
     1921 ttgaaattat acaacagcaa ttcggattat tcggagaaat tcacaaattg cacacttttc
     1981 tattgacata tacgcattat gagtatataa tctaattgtc gaaaaaagta gcgctatgaa
     2041 cttttgaatg tttataagac aaggaataaa gtaaattaag cagatcatgc ttaaaataat
     2101 attaaaaaag gtgatattta tgaacaacaa aggaaaactg attttttcaa cgaaatcttc
     2161 gtttgctgtc cgcaaggcgg taatcggagt atgtgaactg atcttcatta taatggcaat
     2221 tattatgata gtcaattacg aaggtatcaa atccgcattc ggcggcggaa gtacaggtgg
     2281 aataatcgct gtagttgcaa tcctgttctt cattctgtat cccctgataa tgtttgtgat
     2341 gctgtgcctt aaaagcgcat cgtactgtga agtctatgaa aattacatag tcgggaaaac
     2401 gttgtcgctt caggaatttg aaatacccat gaatgaagta atcaacgtgt cggaagaaaa
     2461 aatgacgctt atcatatatt cgcagtacac aaactacaag gtcatggcaa acacaaaccg
     2521 tgcagaagca ctcagggaga tacgttcgag aataggtaaa taaaataaag cacagcaaca
     2581 cggtttgcca ctgttgctgt gccggatttt gttaataccc tgcttattgc agggttgttt
     2641 ttctgtagga acggcattac agcccgcttt ttcacatgga ttacggaata ccgttttcca
     2701 aaataactgt tttaaagcat aattatatat agcaagaatc cgtattgcaa tttaaagatg
     2761 aaacgaggta aaacacatgg cttatgcgga attgtcatat gagaagtacc tgacgtattt
     2821 caaaacactt acgagtatag aattaaaaaa gtatgaagca acagaaatat gtaaaaaaat
     2881 cagcagtaaa atctacacac ttgaaaaaga taacgatgct atcagcacaa gctgtgtttc
     2941 ctgccgtaaa ccgttttacc aaagcgcgat cagtaatttt ttcaaagtca tcgggtctgc
     3001 tttgatttac ggcttgtacg gcggaattgt cggagtggct gtaggactta tagtcagaat
     3061 gatatattta tcaaataaaa gctactctat cctaagctac atatggaact tgctttttgg
     3121 ctcaattgac ggtttagaca tatcgctgtt acaattcttg ttattaggct ttgcaacagt
     3181 cggaattatc gcatttgcag taaaattcat acaacgtatt tcatacaaca actcgcttcc
     3241 cacgcaaaat gaagttttaa aaaagcaaca aacaaaaatt gcttataata aaatctgcat
     3301 aaaacgcctt aacgagcaga agcagtatta taaaaagact attgacgacc ttgaaaattg
     3361 tcttgttcga tactattctg tcggcttaat caaccgtgaa gcattcggcg gacttgttgc
     3421 cacaggaacg atatacgcct acttctcaaa caaaatctgt aatacgttgt acggggacaa
     3481 gggtgcattg tcgaggtatg actatgaggt gaaataccgt attatcattg ctaaactcaa
     3541 tgaaattata gacgctttac acactatcga aggcaaccag cggctgctcg ccgatatgct
     3601 tgaaaccggc aacagaaaca tctcaaaaat gtgcaatacc gttaatgatc ggttggaatc
     3661 cgctaattat aaactcgata caatcaacta ttgtcagaac gtaaataacg ataatatgaa
     3721 atatcttaca tggctcaatt ctggatattg gaaattctga gagccgttat ataaagagtt
     3781 ttctccggtt ttaacagata agtcaggcgc agtaactttt tgcatatcgg ttactgtgcc
     3841 cgactttttt aaagctcaat ataatgcgga atgatatcct caaactcgcc gttactaagc
     3901 ctgaagcccc tcggaatgat accgagctgt gtaaagccga gcgacttata cagtgccagc
     3961 gccgcagtgt tgcttgccac caccgcattg aactgtaaaa taccgaagcc cagctccctg
     4021 gcttttttga tacagtcggt cactaccgct ctgccaacac cctttccgcg ggcagaagag
     4081 cttaccgcat agcttgcgtt gcttatatga ccgcatctgc cgatattgtt agggtggagt
     4141 atgtagagtc cgtatatcct gccgctttca tcacaggcaa tgccggtgta gctctgtgag
     4201 cggaaaaatt cgtccgcttc tctgtcgttc atttcgttct cctgcgggaa cgcaacgcca
     4261 tcgcgtacta cctcgttcca tatttcagcc gcagcgtgtg cgtcctcgct tttgtattct
     4321 cttaccgtaa tgttcacagt atcactcttt tcgcttttat aaggacaatt atactcttaa
     4381 gacaggcaat tgtaaataat acggtaacac acttttcggc aaatgtgcaa tttgcacaaa
     4441 ttatagcgat atatttctac acccgtattt catttttcac gaaaaatccc ttgtattttt
     4501 tccaaaatcc gtatataatg tatttatccg aatttttgat gaaaggatat aaaggataaa
     4561 accatgaaaa cttacacttg caaaaacata cgaaacatag cccttgccgg acacggcgga
     4621 agcggaaaaa cttctgtagc ggagggtctg ctctacaagt gcggcgagat cgaaagaatg
     4681 ggtaaggtat cggacggcaa cagcgtctgc gactatgacc cggaagagat caaaagaaag
     4741 atctcgctcg gcacatcgct tgcttactgc cagtggaaag acgttaagat aaatattctc
     4801 gacacacccg gtcagtttga ctttgccgca ggactttatg agggtatcaa tgccgcagag
     4861 agcgttgtga ttgcgctccc cgcaaaggaa ggcgtacagg tcggcacgat aaaggcatac
     4921 agagaagcgg taaagcacgg caagacgaca atgttcgttg ttacccgtat ggaggaagaa
     4981 aacagcaact tctacaagtg ccttgaagag ctgaaaaccg agttcggacc ttctatatgc
     5041 ccgatagtcg ttccctttga caaatacggc acagttgaga gctacataaa cctgcttgaa
     5101 atgaaggcat acacttacga caacggcgta cctacagaag tgcccatgcc tgcatccgag
     5161 aaccgtctta agggtctgcg cgccgcaatg gctgaggcgg tagccgaaac ggacgaggag
     5221 ctgatgatgc gctactttga gaacgagggc gaggacttca cagaagcgga aatcgtaaaa
     5281 ggtcttcatg acggcattca taaaggctac ataacccctg ttgtttgctg ttcgggcgac
     5341 acgcttgcag gcatcgatat gatgctgaat actataattt atatgcttcc gagccccgat
     5401 gaaacaagcg gcgagctttg cgaggacggc tcaagagtcg gatatgcaag tcgcgacacg
     5461 cttgaagcaa aggtattcaa gacagtagcc gacccctttg tcggaaaatt aagctatata
     5521 aagatagtga acggcactct tgccgcagga acagaagtag ttaaccgcat tacgggaaat
     5581 cttgaaaagc ctggcaagct tctcagtatg cagggcaaga aaacggaaga tatgatagaa
     5641 gcatacgcag gtgacatagt cgccgcgaca aagctcagtg cgaataccgg tgatacgctc
     5701 acctcatcgg gtgacgaaac ggtatttgcc gcagaaaaat atcccgttcc ttgtttcttc
     5761 aaggctatct ccgcaaagga taagaatgac gagggcaaga taagcaacgc gataaagcgt
     5821 atggtcgagg aagataagac gctgagctac aaccacaacc acgaaacaca tcagcgtata
     5881 ttaggcggtc ttggcgaaca gcaccttgat gcggctattg caaagatgaa aacgaagttc
     5941 ggtgttgatg ttatagtatc cgacccgata atcgcatatc gcgagtcgat acgcaaaaag
     6001 gttgaagcag agggcaagca taagaagcag tcgggcggtc acggtcagta cggtcatgta
     6061 aagatccgtc tggagccgaa cgagagcggc aagggatatg agttccgcaa cgaagtagtg
     6121 ggcggcgcta tccccaagga gtatatcccc gnnnnnnnnn ngtcacggtc agtacggtca
     6181 tgtaaagatc gaatttgagc cttgcgacag cgatacgctc gtatttgaag aaaaggtatt
     6241 cggcggaagc gttccgaaaa actatttccc cgccgttgaa aagggacttg cggaatctac
     6301 cgcgcacggc gtacttgcag gctatcctgt agtaaacctg aaggctactc tgcttgacgg
     6361 ctcgtatcac cctgtagaca gctccgagca ggctttcaag atggcggctc acattgcata
     6421 caagcaggga cttacacagg cacagccgtg tattcttgag cctatctgcc ttgttaaggt
     6481 aacgcttgac gatgcaagca caggcgatat aatgactgtt atcaacaagc gcagaggtac
     6541 tgttctcggt atgaacccca gcgaggaaga acaaggtatg accgaacttg acgctcagat
     6601 gccgcagtcg gagatcaccg accttgcgac cgttatccgt cagataa
//
LOCUS       DAWAOG010000039         6622 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1624_length_6622_cov_2.132633, whole genome shotgun sequence.
ACCESSION   DAWAOG010000039 DAWAOG010000000
VERSION     DAWAOG010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1624_length_6622_cov_2.132633"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4336..4345
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5358..5367
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5661..5670
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1614 a         1567 c         1361 g         2050 t
ORIGIN      
        1 gacgaggtga acgtgttagt ggaggaaaat cttccggaag tggactttct gaagaaactg
       61 aacaatctga aacagaaagc aaattaaaat tttccctttc tgaacctgtc gaggaaaaga
      121 acgaccttat agctgtgcat aatatctatg cgaacaagat aagtaaatcg ttaaagcttg
      181 gtggtttccc aatgccctca atagcagtca caaaagctga tatgggacac gaaaattatg
      241 gtgaatgctc ctttgttttt aacaaatcaa cgattgatcc gaaagtcgat aaaagaaata
      301 aggtttacgg tggtgatgcg tggacaccga cttatcctag catagattat aaagtcaacg
      361 agaatgtaga agataaagca cgaaaaaaat actatgactt atacgaacag tacggagaaa
      421 aagtcaggtc tgcccaaaga tatcttgcat acctactcac ggcttgcccc tcttgcatgg
      481 aacatatcct gccgctatcg ccgcatcata atcattcgtg tagtccgtgt tttcttcgct
      541 catctgctta accgatgaac agcttgcacg gtgtatcttc atagtgtttg tattcagcac
      601 atacgaataa cttacagatt cagccgccgc aggcgcttgt gttgcaggcg caggtgttgt
      661 gactaccgta gttgtttcgg gcttgctctc acctgactgt gcaccgtctg ctgtggtcgg
      721 cgatacattc accgtaatat tcttgccgtc acacgtcatt acaatatcgc cctgcaaatc
      781 ggtacggtag atatttacgc ctgccttttt cagcttgtcg atcgtttcgg tatgaggatg
      841 tccgtatgaa ttacccgcgc cacaggatat caccgcatat tccggacttg ccgctgtgag
      901 aaatgcactg ccggtagacg tactgcttcc atggtgtccg accttaagca catcgcaatc
      961 tatatcggct gtaactgtgc gttcttcctc ttcctcagcg tccccggtaa acagaaattt
     1021 cctgtcacca taagtcagca ttatcataac ggatgaattg ttaaggttgc tgtagtcctc
     1081 cgttacaggt gcgatcacca cagccgacaa atcaccgtcg gagaacagct ccgtacctgc
     1141 agtaccaagc gttacctttg caccgctgtt gtcaatcgcg gtcagcatat tcgtatatgt
     1201 cttggtagtg tgcgttgcgg gacttaatat aacatttcca acgctgaacg aacctataac
     1261 gtctgccata ccgcctatat ggtcgctgtg cgggtgcgtt gccacaacat aatcaagcga
     1321 attatatccg caggaatata tatatgtagt tatcctgtcc gcatactctt tttccgatgc
     1381 gtcaataagc atagttttgt tatcgggcag ttctataaat atgctgtcac cctgtccgac
     1441 atcaagaaaa tgcaccttaa gctcattgcc tgtcgtgctg cttcccgaac tttcgctttc
     1501 ggtttcttct gccgatgacg gctcattact ttccgacacg attgttttat ccgacacagt
     1561 ctgttctgtg ctttctgccg ctgtcgtttt atcggatacg gtcggctctt cgctgtctga
     1621 agatctgtcg tcacccgggg acgattctgt tacagctgta ccagttatat tctctgctct
     1681 gctttcaggt gatgaattta cctcttttgc agagcagccc gacaacataa tcagagcaag
     1741 cacaagtgcg gttattttct ttttcgtatt atcactccct ttcctataat attatcacaa
     1801 attatcagct ttgtctacag ttaaaaatat atctgtacaa tttttcgtac actgatgtaa
     1861 aacgtgcttg taaatatgca aatcccctgt cgcgtttaat tcgacagggg atattttcat
     1921 tgaaaacgat ttattcactt ctgagtgcca gtacaggatc tttcttagcc gcatagtgtg
     1981 acggaataag tcctgcaata agggtaagga gcatactgat tattaccagt ataacagcgc
     2041 cctgccacgg cagtacgcac atacctgaaa tgccggtgag gttgtatatt attgcgtttg
     2101 ccgggataag cagaagcagc gttacaccta taccgatagc gcctgcggca aagccgacaa
     2161 taagcgtttc tgcgttgaac acacgggaaa tatctctctt gcttgcgccc atagcacgca
     2221 gtataccgat ctcctttgtt ctttcgagaa cggaaatata ggtgataata cctatcatta
     2281 ttgacgatac aacaagtgag atcgctacga atgcaataag gataacgctt atagcatcga
     2341 taatagtaga taccgaggtc atcatcagtc cgactatatc ggtatattct atcttgaggt
     2401 tttcgttgcc gtcagccttt actttgtcgt tgtagtcact tatgatctgc tcgatctttt
     2461 cctttgagtc gaaatccttg ggatagatat ttatacgact gggctcatca agcgaagcca
     2521 caccgaggag cgtcatattt ccatcgtagg ttgcgtcgga ctctgtgttc atctgctgcg
     2581 agaatgcatc gactatctgc tgttcggtca ttcccatctg ctgtgcctgc tgaagctgtg
     2641 ccattacctg agcctgctgt tcttgcggta atgcagctat atacgcctgt atatcttcca
     2701 ttgtgagggt tttcttgtcc tcgtcctttg caaattcctt tcctgtgaaa atatcggtgt
     2761 cgggagagtc tttctgtgcc tttactatct cgctgtcatt taccttgttt acgagatact
     2821 cggtaagagc gtgggtataa ccgatagctg aggaagagct tgacgacatt atgctgttct
     2881 cttcaggctt gatgatacct acaaccttta tcggttctgc gtcctcgaca acctttgtca
     2941 tataaatctt gtcatcgctc ttgtctgtcc agataccgtt ttccttttcg tagaattcgg
     3001 ggtttgtcac aagtctgaat tcaagtccga gaagctcgtc ataggtgtaa gtctgtaatt
     3061 tgcttgtgaa ttcgaccttt tcgttattgt tgctcatagc gctttttact gcattgataa
     3121 gctcttcgct gtccataagt cccatacagt aaaggctgta gtcggaaatc tcgttatcct
     3181 tgtttaccat tacgacaact tcattgtaag cactcggcat tcttcctgca agcaccttgt
     3241 actgctcgtt taacagctta tcattgtcaa gaagctcagt ccaggtgtca tagcttgtgc
     3301 ttacaacgtt gctcataagc tgtgagtcgt acattgcgtt agtcataccc atatcttcaa
     3361 acagggtagc ggggtttacc tgaattatgc ctttatcctt atcgtttctg aaaatattga
     3421 gcggagtgga atagccgtac ttgatatcgc ttacataatc gttgatttcc ttgccgtcat
     3481 tttcaaggta agttttgaag ctctttaaat cgttggattt aagctgagta agcatggtgt
     3541 ttaccatact gcccattatg ttattggagt gaacttttcc gtcgtcgttt ccgtcacttg
     3601 tgttgtcttt catcattgaa gtcatcatag acgacatatc catagcgttg cgttctatgg
     3661 caataggata gctggcgagc gtttcctcct cgaccgactt tatataaagc tgcgcaccgc
     3721 tcgatattga cagaatgagt gcaatgccga taatgcctat gcttcccgca aaggaagtca
     3781 taaaggttct gccctttttg gtcatcaggt tgtttaaact cagtgacagt gcagtaaagt
     3841 atgacatcga cggtttcttt atcttctttt ccttacccgc ctttttcttt tcgataggct
     3901 cgactacatt tctgtcgtaa ggattggtat cgtcaactac cctaccgtca agcagtctta
     3961 ttattctgtt ggaatatttc tcggcaagct cggggttatg agttaccata atgataagct
     4021 tatctttgga tatctccttc aaaagctcca ttatctgaac gctcgtttca ctgtcaagcg
     4081 cacctgtcgg ttcatcggca aggagtatat cgggttcgtt tacaagcgct cttgcaatag
     4141 caactctctg catctgaccg ccggacatct gattcggctt tttcttaagc tgatccccaa
     4201 gtcctacctt tacaagcgca tcctttgccc tctgtcttct ttcggttttt gatacgcccg
     4261 ataaagtcag tgcaagctca acgtttgcaa gtaccgtctg gtgcgggatc agattatagc
     4321 tctggaacac gaagcnnnnn nnnnngcgat atgtatccca gtcggcattt ttaaattcct
     4381 ttgtcgattt tccgcctatt ataaggtcgc cttccgtgta tctgtcaagt ccgccgataa
     4441 tatttaaaag cgttgtcttg ccgcagcccg actgacccag tattgataca aattcactct
     4501 cgcggaaatc tatgctgacc cctttaagtg cgtgtacctt ttcatcacca accttataat
     4561 ctttggttat gtttttcagt tgaagcatcg taatatcctc ttcataagaa ctttaaagtt
     4621 atatgtaaac ggcaaaagcc gctttatagt ctgctaaaca ccgtgattgt ttattttaat
     4681 ccggctgtgt taaccgcatt taaacttttc tttttcttat cttccttatc tttgtcctct
     4741 atcggaagat aaacggtaaa ctgcgtacct ttatcgggct cactctttac tcttatcctg
     4801 ccgccgcaaa gctcgacaat tcgctttacc atagcaaggc caagaccgtt tccgccggtt
     4861 gcgtgcgagc tgtcaccctg atagaacttt tcaaaaatac gttcggcggt agccttatcc
     4921 atgcctatgc cgttatcggt gatacgcact cttacttcct gttcattctt cggattgata
     4981 tcaagcttta cctcgatcgt accgccgtcc ggtgtatact ttacagcgtt gccgatgaca
     5041 ttcagccaca cctgtttaag cattccgctg ttgccgtaat aggtaatctc gcaaagatcc
     5101 ggaataacct cctgattttt ttctccccac tgcccctgta attgcagaac gcacctgcgc
     5161 agttcctcat cgagcgaaaa atctttcttg tcggtgatta tttcctggtt ttcgagctta
     5221 ctgagcataa gaatattgct tgacatattt atgagccgct gtgactcgtt tatgattatg
     5281 tcaatgtatt cctgctcctg ctccttagta agtccgcctt ttttaagctg ttttgcaaag
     5341 ccgtaaacag acaccatnnn nnnnnnnctc ctttaccatt atattgaagc tttcctgtat
     5401 atgcgaaagt tcgctgtttc cgttttcctc aagtcgtaca gtaaagtcac cctttgatac
     5461 tttaagcaga ccttccttga gatcattgag cggacggagc agtatacccg atagcatagc
     5521 cgacagtata ctgccgatta ttatacaggc gcacagcata ataaacggca gtagcatcgg
     5581 tactacctct atatgcttaa tcagtccgaa tgcgtcaagt atattgaata tcagcacgca
     5641 gataacgcct gccgatacca nnnnnnnnnn ccattatgaa gaatacgaac aatacgacat
     5701 aggtatttat ggagcgcagt cgcttttcct catcactgat ttttctgtgc atcagaatcc
     5761 tcctgacact taatgctgtt tcagcacagc cttgtagccc agtcctctga cagttactat
     5821 ctcaaaatcc gggttgtcgt gaaacttatc acgcaggcga tttatatgaa catctatagt
     5881 acgctcgtcc gtttctgttt ccatatccca cagctcatcc ataagctgac ggcgtgtgaa
     5941 tatcttgttc tgatagctga gaagcttgaa cagcagcata aactccttgt tcggaagctc
     6001 atatgtattg ccgccgcttg tcactgtgag cgcatcatag tcaagtaccg tgccgcctgc
     6061 gtggagcttg tgctcattcg ctatattact gcggcgaaga agtgccgata tacgaaggag
     6121 catttcctca tcgtcaacgg gcttcaccat atagtcgtca gttccggcac ggaaaccggc
     6181 gatcttatcg gcggaggttt cccttgccgt aaccataagg acaggcgtat tacagccgcc
     6241 ctcacggagc gttttgacaa actcatagcc gtccattctc ggcatcatta catcaagcag
     6301 tataaggtca acgtgctgtc tgtcgagtat atcgagcgca tcaacgccgt caacggcagg
     6361 tacaggctca tagccgtttt gcttaagcac cgtgcacatc agctttctgg tatttgtgtc
     6421 gtcctctact accattatat ggaacatatt atttcctccg ttttctttaa gcaaattgaa
     6481 ctgtatagat tatattctcc ttgatttaac cgaatattaa gtttatgtgg tatgaaccgc
     6541 ggtatagttg tataaaatgg ggttgtggca caacccgaaa aaagaaaaag tgagtcgaag
     6601 cgcaaaggat agtgcgtaag gc
//
LOCUS       DAWAOG010000040         6521 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1644_length_6521_cov_2.419734, whole genome shotgun sequence.
ACCESSION   DAWAOG010000040 DAWAOG010000000
VERSION     DAWAOG010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1644_length_6521_cov_2.419734"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1172..1181
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1857..1866
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4325..4334
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1864 a         1418 c         1462 g         1747 t
ORIGIN      
        1 aatctaacag ttgaacaaaa tgcggtaaaa cgatataata tataaagaaa ggaggttaat
       61 tatgtcaaaa ttgaaaaagg taattcaaaa ggtatcaacg attactttgg cagttgtgac
      121 attaagtgta tgtgcgatcg tagcacaagc tgagatcaat gtgtgaatat aaaccacaca
      181 ggaggttatc aatgcgacca aaatgcagat gcatttatca cgataacaaa agaaaaaata
      241 ggtacaatgg cttattgtta cagcaattct gtttatatca gagcaactac cacaacagga
      301 ggtattacat cgcaaggatt tacagcaagt ttgtccaacc ctaaacgttg tgacagaaaa
      361 ggaaatttca caaacggtgt tgttagcgaa aagattattc ttaaaggtga tgtgagcaga
      421 tatacggtaa ataccggatg gttttcaagc accgagtacc taaggattac aaacacaaat
      481 tctaacgatc agggaagcaa acaaactttc acaaaacgtt acaatgatca ttgcgcttga
      541 tattaaggag gtatttatat gaaaaaactt ataacgcttg cactggtaac aataatggcg
      601 cttatttgca ctgcgtgtgc aaatgttgca gctgataacg atgtgtcggt cgaaaagccg
      661 gaacttaaat atggcaggta ttatcttaat ggtgacaaag aaagcggtct gtatgtagat
      721 ctggatgaaa atttcatagt tatgggcgga gaaaacctaa gaaagcatca agaggatttt
      781 tgtcgccgat atgcaggaga tacttactcc aatgaggaac agctacagga taatgtagat
      841 gaaataatgc ttgcatatgg cagtaaaagc gagtataatt acacagttct tcaggtcggt
      901 tcagattatc acaattatat ctttttcgac tatttccatg atggctcgtt tgataaaagt
      961 aaagacagcg gtgctgctta tgagtatcct aaggtaggaa tgttgaccac ggataatgat
     1021 gatttttatc ttgtgcaata aaatcggaac atttgacaag tatgctgtta caggtattta
     1081 ataaagacgt tgaaaaaagg caaaatcaaa gttttagaaa aagaatttat tttggtttga
     1141 ttatccggaa tatttatatg cgccatgcgc cnnnnnnnnn ngcgttgttg tttaccacag
     1201 ccacattcaa gtgaaagatc cgtgaaattg tgcttataga caaaatccgc ttgaaatagt
     1261 gcggatttta tgttaaaata aaataagtat gttataggct acttgatagc agaaaagagg
     1321 tacaaattgg atacatttgg tgaacagctt gttaaaaagg ctacgacagg tgctgactgg
     1381 gcaaagagaa taggtcttac ggttttagga ctggttgttg ctaccgttct tatgtggctg
     1441 tcattcttta caggatttat ggtgctgacg cttctttcgg taggagcgct gttcggactg
     1501 gtatggcttc tgacaggaat gagctatgaa tatgagtata tacttacaaa tgacgatctt
     1561 gatatcgaca agataacagg caaaagaaaa aggaaacgtc ttatcacatt aaagatgaat
     1621 acggtagagg aatttggcat atatgacggc actaacggtg caaatgccga cgcgaccgtt
     1681 atcgcatcgg acggaactaa tataaatgcg tattatctta ttgcaaagca caagacgcac
     1741 ggcagaacaa tgctcatatt ttcacctgat aagcgtatgt cggaaatgat acttgaatca
     1801 cagccgtaca aagtaaagca ggaagcaaaa cagagtatag agtctataca gcaaagnnnn
     1861 nnnnnncaaa gcgcagaata attaactgtt tgtcggaagg actgatagca acggtacgca
     1921 ataagaaaaa taaaataaaa gctgcttttt gctgtatgct tacggtgtct gtcgttgcgg
     1981 taagcgcagg ttgcggtagc gtagacctca caatggacgg cagaaacagt aatgtacaag
     2041 gcggagccaa gcccgctgtt agtgttgtaa agccgataca gacaactgcc gaaacgacaa
     2101 caaccaccac tacaacgaca gcggcaacta cagaagctcc cgaacccgag ccggtctatg
     2161 gcgacgattt tgtaaactgc tatgcggcaa tatgttacga ccttacagaa ggtaagatca
     2221 tatattccaa gaatgccgat gagcttgtct accctgcaag caccacaaag ctgctcacag
     2281 cgctgacggc gatagaatat acatcacccg agtttatata ctatgtcgga agcgagcttg
     2341 agcttgttgc gcaggactcc agtatgtcgt ggatagcgca gggttcaact ctcagccgga
     2401 atgcgatact gaccgcaatg cttgccccct ccggtaatga cgcggcatat acgatagctg
     2461 tcaatgttgc aagaaaggtg tacggtgatg atatcagcga cagcgaggct gtgtcatatt
     2521 tcgcggtgct gatgaatgac tatgcaaaaa agctcggagc agagaacact catttcaccg
     2581 tccccgacgg ttatcacgat aacgatcatt acaccacagc gtccgatatg ctgaagcttg
     2641 ctatcgcggc aacgcagagc agtacaatat gcgatatcac ctgtcagccg cttgcgatag
     2701 tatatgacga agagggcggc gttcacagct gggacaacgg aaatattctg ctcacagata
     2761 caacaatacc gtatgaagta tatggcttta aaacaggctt taccgatgag gcgggcttct
     2821 gctttatcgg ttatgcaggt atgaacaata agaagatcat tacgctgaca tacggctgtg
     2881 aagtcgaaaa ccgctttgct gatacaaaga agcttatgga tctcggcttt gatatttatg
     2941 acgaaaacca cgactactac agtgtagccg aatactaagg aagacaaaat gaagatatta
     3001 tcgatagaaa gctcctgcga tgaaacggcc gcggcgataa ccgaggacgg cagaaaagta
     3061 ttatcctctg tgatagccac acagatagat gagcataaat tgtacggcgg agttgtcccc
     3121 gaaatagcat cgcgcagaca ttgtgagaac ataacgggcg tgtgcgcaga agcgcttgaa
     3181 aaggctgata tgacgcttga tgacgttgac gcagtagcag tgaccaacgc ccccggactt
     3241 ataggcgctc tgcttgtcgg tgtgaacttt gccaagggac ttgcacttgc aaagaacaag
     3301 ccgcttattg cggtgcacca cataaaaggt catattgcgg caaattacat aacccacccc
     3361 gagctcgagc cgccttatct gtgccttgtg gtatcgggcg gtcacagcca tattgtaaag
     3421 gtaaacagct acaccgagct tgaaacagtc ggcaagacta ccgatgacgc ggcaggcgaa
     3481 gcgttcgaca aagccgcaag ggcaatgggc tttccttatc ccggcggtgt acatatcgac
     3541 agagcggcaa agcagggtga cgcgaagatg tataagctcc cccgccccgc aacgaaaaat
     3601 ccgtatgact tcagcttttc gggacttaag accgcagtaa taaaccttat ccacaacaac
     3661 gagcagaagg gcatcaaaac cgatgtaaat tccctcgccg cttgttttca ggacactatc
     3721 acctctattc tggcggaaaa gtttatggcg gcggcaaatg agctaggcta tacaaagctt
     3781 gcgcttgcag gcggcgtagc ggcaaattcc atgctccgtg ataagctgtc ggagatggca
     3841 aatgcaagcg gaaagcagtt ctatatgccc gatatatcgc tttgcggcga caatgcggct
     3901 atgataggct gtcagggcta ttatgagtat cttgcgggaa atattgcgga tatgtcgctt
     3961 aacgcatatg ctacaaaaaa gctgcatatg ctttgattta aaacaaaaga atatgatctg
     4021 agccacgact atttgccgtg gctcgcagtc tgtcgaaaga cctatttaaa cattgaaatg
     4081 cggtgtcggg tgttgcttgc tctgccggtt ttgcaactct gcaaggtcag ctgtttgtgc
     4141 cgttgcatca tacaagtgct gttgttttgg ttagtttttt catggcattt ccctccattt
     4201 ttttataaga caagatttgt tatacaatga tattgaatcg cttatttttt cattgctgtt
     4261 tctcataatt gagtttggtg cgaagagccg aaatttgttg tactttcctt gctgtataaa
     4321 gatgnnnnnn nnnncttgct cctattatat tgctttagta aaagtgtact atacgaaaaa
     4381 agcgagacga gcgcactatt tttgcgcttc gactcacctt tccttttcca ggttgtgtca
     4441 cagccatata tatacctatc ctttttcctt ttccgggttg cgtcacagcc ccatatatac
     4501 ctatcctttt gttatataca gcctctcagt catcgtattt tcgcagaaat acctgtcatg
     4561 ctccgcaagg agcatagtcg gcgtattcac accgagtagc cgttcgagct gtatccttga
     4621 aataatatcg acaaagttca gcggctcgtc ccatatataa aggtttgcgg gcgtggcaag
     4681 actgcccgct ataagcacct tctttttctg tcccgcactt agattttcgg ttcgtccgat
     4741 aagctccttg cgagaaaaac cgagcttcgc gagtatcgca cggacaagct ccgggtcaac
     4801 accgagccgc tctgaaaatt cgtcaagcgt tcctgaaagc ccatttgtat cctgcggcaa
     4861 taccgatatt ttaagcccgg gcgcttttgc tatacttccg ctgtagctta tatccgcccc
     4921 tgcgatgatt ttaaggatcg tagattttcc gcagccgttt ttgcccgaca gacacagcct
     4981 ttccccgtaa gacagcgtaa atgaaacagg ctcgcataaa gcattgccgc aataatacgg
     5041 aacaaggttc tgcaccgaaa gcaccgttgc cgatttagcc ctgatcgagg ggattttcag
     5101 cacatcaata tattcgcggt ttttcagcag ctccgatttc tcgtttatcg ccgactgtct
     5161 gcggttttcg acagctttca tacgcttcat aagggctttc gccttagctc cctcaacagc
     5221 tctcctgtca ggcgactttt cggtttttgt cggatcgatc cctatctttc tgctctccgc
     5281 cgtatcagcc cactttttac tgcgttctgc ggcggctttg agatgcccta tctcactttt
     5341 cagcttcgcc acatcttcga tgatctcata agtctgtgtc tcccgcagtg tgaaatcata
     5401 ttttctcgca ttcagcttct tgtcacgcat aagctcgttt gcttcttttc tgtcgaagtt
     5461 ctctttccac acgctgtagt taccgtttat tatttcgata tcactgcggc tcagcgacag
     5521 tatatggtcg gtgcatttgt caagcagatc acggtcgtgt gatacaagta taaatccgct
     5581 ctttgctttg agatagtcgc ctatcacctc tcttgcgtca aggtcaaggc agttggtcgg
     5641 ctcgtctatc agcagaaaag cgttgtcgcg caaaaacagc aggcacagca gcagccttgt
     5701 ctgttcacca cccgacagcg tgttgaacgg tctgtacaga atttcgcagt ccgtacccat
     5761 ataagacagc tcacggataa cttcccattc ttcgactccg ggacatatca ccgcgataag
     5821 ctcactgcct gtcatatcct tgtcctctac atcaaaagga aagtacagga actcagcctt
     5881 tgtgctcacc gcaccgccgt tttcaagctc gcctgtcaac agcttcagaa gtgtggtctt
     5941 accccgtccg ttcctgccta taagtcctgt tttccacgtt gtgtcaagcg tgaatgacgc
     6001 attctcatac acggtttcat cacagccgtc atagtaatag gttaaattgt ttacactgat
     6061 tatagacata atatcatacc cctttctgta tcaaaaaagc cgcaggaaca tacgctccca
     6121 cggcttttta tcccgatctt cgcataaaca cacggcgaaa aagggcataa tacaagaccg
     6181 acagcaatac attcctgcat tgcagacacg aaaccaagcc cgttttatcg cccgtcttat
     6241 catatagggc ggcaagggct ataagggtat ttcgttctgc tcttgtcagg aatttgtacg
     6301 cattgcattc ggtcctttcg ttttgaattt agaatatcgg aacttccgat acaagcggat
     6361 tataacacag cttactttat ttgtcaagag gcaggcggga aatttaactc cggcattgag
     6421 cagcttacgc cagaatgtag atgagttgac aaaaaatatc taatatgcta taatttattt
     6481 tacagggata ttttgcctgt attttacatg gaagccgaat g
//
LOCUS       DAWAOG010000041         6502 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1648_length_6502_cov_2.019854, whole genome shotgun sequence.
ACCESSION   DAWAOG010000041 DAWAOG010000000
VERSION     DAWAOG010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6502)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1648_length_6502_cov_2.019854"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    690..699
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3439..3448
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5265..5274
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6095..6104
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1587 a         1609 c         1307 g         1959 t
ORIGIN      
        1 gatatacgca aggtgcgctc ttatctgatg cgtcctgcct gtgagcaggt cgacctttaa
       61 cagcgagttt tcgccgttgt acgctatgac cttgtacttt gtctttatcg tgagattgct
      121 ttttgtcttt ttgtcgctga tttttaccgt gttggtgtcg ctgttctttt caagatatgc
      181 ggtaagcgtt gcgctgtcgg gagttacctt accgcaggca atgcacagat acagctttgt
      241 aagctccctg tcccttatct tctgatttag tattctgagc gcctccacat ttttcgccgc
      301 gatcactatc ccgcaggtgt tgcggtctat gcggttgcag agcgcaggcg cgaaagacag
      361 ctcgctctca gggtaatatt cccccttgtg ataaagatag ctctttatgc ggtttataag
      421 cgtgtcgctg tctccgctct cgtcctcgtg cactaccatt ccgacaggct tatccacaag
      481 cagaatgttc tcgtcctcat agacaatgtc gatcttatca ctcaccgccg agaaatcatc
      541 gggatcagcc tttgctttcg gtatcagcat atcatcgggg ataaacagtt ttattatatc
      601 attttcctca atatgagttt cgggcttgca tttcttgccg tttagcttta tgcagttctt
      661 gcgtgccagc ttgcatatct gtccctgacn nnnnnnnnnc tgacccagcg gaaaagattt
      721 agacaaaaag cggtcaatac gctgaccgct gtcattcttt gttaccttta tttctttcat
      781 ctacggctca cttctgtatt cttgaaaagc cgtactgcga gaaatgctct atcgccttgt
      841 tgaagtaatc acctgagctt tgacaagccg ttgcaacatc ggtgagtttc gcttttgcta
      901 tctcccactt ttcctgcatt acctcgttat catcggcttc aagccagagc tggttttccc
      961 agataccgta tttaagattg attatatatg cttttttcat gtttctcctt ttttcctacc
     1021 ggatttttta tttcaggaaa gcctctgaat acgggagggc aagcccactc ccctacaatt
     1081 tttaggattc acagcaatta cagctttccg cccttagctc cgccgagctt accgcctgta
     1141 aaaccgtcaa gaatgttggt gtcaaagctg aatgtcagcg ccttattctc acgttcacgc
     1201 tttaatgcga gcgagatcat ctcgtctaaa agctcaggat atttcacgcc gacaggctcc
     1261 cacagataga acgcaaggct gcccggtatc gtgtttattt cgttcagata aacgttgccg
     1321 ttgtccatat ctatcataaa gtcaattctc gatgtgcccg atgcgccaag acacttgaac
     1381 gcctttaccg caagggtgcg tatctgctct cttgtttcat cggatatatc ggcagggatt
     1441 tttcttttca gcgttgccat accgcttgac ttaccgccct tatcgcccgc aatatactta
     1501 tccttgtagc ttaatatttc atcggtgttt accggttctt cacattccga tgcatgagcg
     1561 gtagcatagt cgccgcatac cgagcagttg atttccttga ggtttgttat agcaggctca
     1621 acaagcagct ttgaagcata ggtaaatgcg ttgtcaagcg cttcgtcaag ctgttcgtca
     1681 ttgtgtgcta tcataattcc gacactcgag ccgagattta tcggctttac tataagcgga
     1741 tacgcgatct taacctttat gtcatttttt actgcttccg catcctttgc gtagtcgctt
     1801 atatgaatta ccgtgccgtc aaggacaggt atgtcgttat ccttgaatac ggttttcata
     1861 acatacttat ccatacctac agcagatgat attacatcac agccgacata aggaacgccg
     1921 attgtctgta aatatccctg gaatacgcca tcctcaacgt tcgtaccgtg tactatcggg
     1981 aacgcaacgt ctatcgttgc atatacgttg ttgccgaact tctttccggg ataaagcaca
     2041 agctgtacct tcccgccgtc atttataagt atcaccctgt ggctttcctt gagaagcgac
     2101 ttcaaattcc tgtacgatgc gatgtttcct atcttttctc cgacatacat ttcattgttt
     2161 tttgtaatgt atacgggtat cacctcgtac ttatccttgt ttatggaatt gcaagcctga
     2221 agtgcggata tgatggatac ctcatgctca acgctttttc ctccgaaaaa tacacctact
     2281 ctgattttca tgattttatc ctttctttat gtcaattaag tattatctgt gcttgtttgt
     2341 gagctttccg tcctgtagct gagatctctc aggtcaatat ttaccctgac gtaatcgccg
     2401 tatgcaagta caacgctctt tgctttttcg ggaactatat agcagacata gccgtccgag
     2461 ccgagccccg cagagagtgc taatgatgtg ctaaagctct ttccttcgtc aaaaccccac
     2521 gctctgtcgc agcctatatc accgtcatag atgcagttaa ggtcattgga gagccacagc
     2581 ttttcatcgg ttgtgttctt gactttcatg tggaacgcaa catacatata gcccttatcc
     2641 gctccgccgt acataatatc tgttctgaaa acctcatcgc agataaagct gtattcggct
     2701 gtctgagcgg tttcgttgaa gtttacacgc tgtacatcct gagttgttga ggattcttca
     2761 gagacacttt cttctatgta atcgtagtcg acgttgtcaa aattgtcgcc gatcattgag
     2821 aaaattccgc cgaatatgga aataatgata cctattaccg taggcgcaaa gaccactatc
     2881 ataattacag tcgtaatcgt ctttgctatc ttctgtgtgg aggcattctg agagttgctt
     2941 acagtgctct gtgacggctg ttgtgaagcg ttatactcat tctgacgcat ctcttgtctg
     3001 cgtctgtatt cttcctcttt ccttttcttt tcctcacggt atgcctttac gcccgcattg
     3061 ttgtcaaacg gtgcaccgca ctgggtacag cagttctcgt gttctacatc cagctttgct
     3121 ccgcagtaat cgcagaatac gtccatagta tctccctcgt tttacgcata atatcaataa
     3181 ttatccggca gatcgttttc aagaaggact atcttctttt ccccgcccgt ctttatattt
     3241 tcaacctttg caagtgcctc gttaagatcc tttgcaacgt aagttttttc ctcaggatat
     3301 ccctttgctt ttaatccatc ggttatcggc acggcctgac gctcaccgac cgcgataaca
     3361 aagtcgcaaa tttcagccgc ctgctcacca aagcgcttgt tgagctcata ttccttttca
     3421 ccgagctcca ccattcccnn nnnnnnnncg tatcaagtgc cgcctttgca cctgtcggat
     3481 tggaattgaa agcgtcgtca attatagtct tatttgtgcc cttgattatc tgtagtctgt
     3541 gtggaacgca ttcaagacgc ttgacaggca taacaaggtc agcaaacgga acaccgagcg
     3601 tattagcaac ggctatcgca cctactatat tctgtacatt gtgactgccg ataagctttg
     3661 tcgcaaactg cttttcttca ttaccgttgt gaaccgtgaa ctgcgttccg ttctcgctga
     3721 cggaaatatt atccgcatag taatccgttc cgccaccgag cgaatatgtc accttgttct
     3781 tttcacagtc gtgctttgct atatattcgt tatcgtaatt gaggaatata attccgtcct
     3841 cgggcaggct gtccgcaagc tcgaattttg ttttaatgat attgtcaatt gacttgaagg
     3901 tttcaaggtg ctgaggacct atcgatgtga tgattccgtg cttcggcttt actatatcgc
     3961 agatctcctt tatctcgccg acattcttag cgcccatttc gcacaaaaat atctcgtgcg
     4021 ttgcattaag ctgtccgcgc actaccttta caacgcccat agtcgtgtta tagctctcag
     4081 gggtcataag cacattatat tttgcaccga ggagcttttc aagatagaac tttgtacttg
     4141 ttttgccgta gcttccggta agtcctacta cggtaagatc cggaagtccg tctattacac
     4201 gttttgcgtc attgatataa tgacggttta tcattgcttc aatcggctta ttgataaggt
     4261 ttgcaagcat cgccataacc agcgtcaaaa cctgcacacc cattgttatc gcaagcgaca
     4321 gataacccat accgagtgtt aagaacacga tcagcatcaa aagtatataa acgataagcg
     4381 atgttaccgt cattctgatt actctcggag tgaacacaag cttcttcttt gcctttttcg
     4441 gcgctgagaa aacgagatca aggcacagaa aagccgccgc aataaagcag gtagatgcct
     4501 tcatctcgcc gcatatcagg aatgtaagca ccgagcccaa agtaaatata tgacgcagta
     4561 tgacctcttt tatatttttt cctatccact tgaactgctc atcggaatga taggaattga
     4621 gctggaacat atgcatataa tgcacaaaat taagtccgca tgatgcaccg agcgccatac
     4681 cgagtacagc taccgcaatt gtctgtaaaa tatccaaaat aaactctccg ttctgtatta
     4741 ttttatatca agaaatgacg caagcaccct tgagaatgta aaccaaccct caatgaacgc
     4801 ataatgtccc gcaccctcta ttgttacaag tccgctgtca ggtataagct cgctcatttt
     4861 aacaccgtca gaaagcggtg tcgcatcgtc tttatcgccc cagataagca gggtcgactg
     4921 ctttatttct gataaaagct cggtcagatc ctcgtttaca acctttacaa gtgtagctct
     4981 cataacggga gaagccgcca gatagtctgc actgccgcgc ttttttctca tattttcaac
     5041 agcattcggg aaaagctttt tcataggtgc tgtgctcatt acagctttac ccatcttgta
     5101 aattctcgtt tttatttttg atgatgtgct ttgcttaggc tttattcccg cactgtctgt
     5161 aagtattatc ttttcggggg taagcacagg cttttcccgt gttaccgcct ttattataac
     5221 tcttccgcca aagctgtgac cgaggacaac agtcggatca atacnnnnnn nnnncccacc
     5281 gatgacggca gcgctgccat ccagtacggt gggccggcgc ttcatcatca gacagcagag
     5341 aatttcacac actccaccat tgtctattat attattttta cgctttgatg tcaagaaaaa
     5401 taactgtaaa ccggaagtaa aacttctgcg ggcaatacaa aaatcaccgc cccgaaaggc
     5461 ggtgacaaca acagcaaatt tactgttctt cgacctttgt gttctttccg acaagaactg
     5521 cgacagctgc gccgacaagc agcgcggcaa aacccgcaag cgtttcaagg ttaaatccga
     5581 agccatcggg gaacaccgca taaagcgagc cgagcacaag tcccataatt gcgctgtata
     5641 caagcagaga gtaacgcttc atcagtgcgg atatcagctt tgctccgaat acaatgccga
     5701 taaacacgcc gatagctgtc ggtatcagta tcataaagtc aaggctcttg atagcaccga
     5761 taacagtcgt gtatattccg agcagtacca taacaaacga gccgcttaca ccgggaatta
     5821 tcattgctat tgccgcaacg gtaacaccga gcagaagtgt tataaacagc acagggttca
     5881 ttttataggt gtaggtaaag ctgtcttgaa ctattgagaa tatatcggta tccgcgggca
     5941 gggtcaaagt gatgctctga ccctttggaa tttcgctgcc ttcagttccg acaagagtag
     6001 tcatatcggc agaggatttt tttacggcac agccggtaag ctctccgtct atcttttctg
     6061 cgaccttaag ctgccagtct gagttcatat catannnnnn nnnngcactt atctcaaaca
     6121 cctcattctg agggagcttg tttacaaatg tcataccgac aacaagcgca agtgccggga
     6181 taaatgcaat aaggcatttc ggcgtgatct ttctgccgcc gactgtcata ttgcggtaga
     6241 taagcgggat acttccgatt ataagtccca tgaagaacat attcgttgca acggggaagt
     6301 tatcaaacag ccacgttatc acaaacgaaa agccgagcac tccgacagcc gcgcctatac
     6361 cgaaaaaaat tatgaacttg atattctttt tgattgccga cagttttaat gtcagaacgt
     6421 ctaccatctt gtcatacacg ccgaaaacta ccatcatagt tccgccgctt acacctggga
     6481 tcacgtttgc aacaccgaat ac
//
LOCUS       DAWAOG010000042         6447 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1659_length_6447_cov_2.587766, whole genome shotgun sequence.
ACCESSION   DAWAOG010000042 DAWAOG010000000
VERSION     DAWAOG010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1659_length_6447_cov_2.587766"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    394..403
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1905 a         1351 c         1254 g         1927 t
ORIGIN      
        1 taattatgca ggctgtttaa ttctgtgatt gtgggattat cagtgtgctt atgaatgttt
       61 ttaaatgaga taagcccggg gtgtgatccg ggcttatttc ttttcctgat aaatctgata
      121 acctttgtac tatatgaatt gtccgcataa accttgctgt tgtcataacg cattttacgc
      181 tgtttacgta taagacgcag attgacaaga ttgctgatta ttttcatagc ggtatcacgc
      241 tttatgcaca gctcatcggc aaggtcgttg aagcttttaa agcagtaacc gagcttgctg
      301 tcaagcgatt taataataag gatatataag cgcaattcga tcgctgttaa gtttttgccg
      361 aacacatttc gttctattcg cgtatagtta gccnnnnnnn nnntattctt ttcgggattt
      421 aaaacatatg tatatgttcc cttgcgatta tcctttcggt aagccgctaa aatgtgtgta
      481 atgtagccct ttctcattaa gctatcggtt atcttggata ctgtctgcgt agactttata
      541 ccgcagtgtg aagctattgt ctgctgtctt acacgcttat attcgtgtcc tgctttgctt
      601 cggatatttg aataaacaga aagcaggtat gtataaactg caaactcgtt atgcgtcagc
      661 tcgtttgagg taataaccga gttagacagc agaaaataac ttttcatttt cacacatcct
      721 tgtattttgt tgtattttaa cgtttaagcg gttttgtcgc ttgtacagta ttcttctcat
      781 tcaacgctct gaacgccttt aaatcgtcgt taaagtcctt gtttaatggt atatcttttg
      841 aaacagcata tcctttcaga gcatacttgt cgctaagctg ctcagtagcg tgtttacctg
      901 catcatcatt gtcaaggcag aacgttattt cggcaatagg cttattcttt aaaaactcgt
      961 caagagcaac atctgatttg ccgccgagcg atagcatagt caactgctgc caagcgtcct
     1021 gtccgttgtt tatagcgtaa aaggacgcat aggacagcat atcaattaca gactcgaaaa
     1081 catacagctt attcttattt gtaccaagta aagaaaacga atatttttta tcgcttcccg
     1141 gcaaatcgac acggtatgta gagctatcag atacagaacg acaaaacgca tagcgtgaca
     1201 taataccgtc gccgtttacg gcgatcatat ttacatattt tttagtaatg ccacgctcga
     1261 taagctccag tatatgttca ccgctaaacc cttcacaaat atatttctca cctttttgtt
     1321 tctgtgcctg aagctctatg gtaacatatt ccggtgcgtt tgtgggtata tcgtttttaa
     1381 aacgataaaa cggagtgttc gaactgtcat atccgacaag ctgaagaccg ctgtaaccga
     1441 taccttgata taacttacgg tcatagatca gctgatttac tatgttatac tccagtccac
     1501 gggttttgca caaatatgcg ataacattgc tgtaatttcg ctgtccgttt tccttgtgat
     1561 cctgttcagg cagtataagt cgatgagctt cgattttttc ttctgacgat ttctttttcg
     1621 tattatattt gggtgaatat tccatttgaa tatcgccgca aagcgattta acggcttcgg
     1681 taaagctttg tccgtatatt ttcatacaaa acgcaatagc gttattacta tgtcaaggag
     1741 cttacagctc ccgaaggcta ctaccttgat acaaacgtac acgctgtaac tcttaaaaca
     1801 gcaaatgcta ctgtatcggt aagcaatgaa atcggaaaat ccgacttcaa cgaatatcct
     1861 tacggtcacg gagctataat caaggaatgg aaacttgaca acggcacagc agatatgaca
     1921 gccgatgaaa tcgcacagat gaaggcagag cttgaaaaga gcctgtattt caccgtaaag
     1981 gacagaaacg gcaagtatat caccgcaaca ggtgcagacg gaatatacac atataacggc
     2041 gacagagctg acatttacga atgtccgtga cacaggcaca ggcaagatta taaaggtatg
     2101 gaagcagttt gatacgatga cagccactga acaggctgaa atcgaaaaca atgtatactt
     2161 taccgtaaca gatagcaacg gagcgtatct caaggtaaag ggaaacagcg gtagctatat
     2221 ctactgcggc tcacagaaaa cagaaacaaa attcgttctg aaaaacggca catttgcaat
     2281 tgcagaactg cctacaggtg actacaccgt aactgagtac aacaatgcaa aggactacag
     2341 tccgaaacag cagactcaga aaatcaaagt agtcagaaat cagacagcaa tcctccgaca
     2401 ggtgcaggcg aggacggctt tatgcagacg gcggttatta tcatatcaac ggtaatgatg
     2461 agtgcggctg ttgtgcttgc attcccgtta tcgaagcgta agagaaaacg ctaagcagca
     2521 ttaaaatttt attgtcaccg ggcggcagaa aagctctgcc gcctttgtgg tggcttatgg
     2581 aggtgtgaaa tggcaaataa aaacaaattg cggcataaga acagtaaatc tgtcttaaaa
     2641 actgcaatta ttcttttcgg cataatcggt ataatcacat ttttgattat aacctatttt
     2701 atttactgcc tacagtgcta cgcttatgat aacagcatac catcagagca gctaagcttg
     2761 tcggtgctga taaaatcaat gccttactgg actaatccaa cattttctgc accttttgac
     2821 ttaggggcaa tattacaaat tcacggctct ctgtggtatg tttatatcgt tcttttgtcc
     2881 ctgttgttgc tttctttatt aggaaagaat tatgacgatt acaaaggcgt ggaagccgga
     2941 agtgccgatt gggcaactaa gcgtgacgaa aaggaaaaca gtgacacgac aggtatccct
     3001 atcgggaacg ggttctatgt aacggtcaac aatcctaaaa attgctatta tgaaccgcat
     3061 aatctcaatg aaatagttat aggcggtccg ggagcaggta aatcattccg caagatcaag
     3121 cctgacatca tgcagatgtt cggtagttat gtagtgacag atccgaaagg cgaattgtat
     3181 cgtgatacag caaaattgct tatggaaaac ggatataaag tgcgagtgtt taacttgatc
     3241 aacataaaga aaaccaacgc atataatccg tttgtttata tcaacgagga acaggacata
     3301 gtagtattgc tccgattata atcgtcgcct tagcaatatt aattatatgc gggctcgtaa
     3361 tgataaaact aatgctccgt acttttgagt tggcactata ttgcagtgta tcgcctgctt
     3421 tttttgcatg cctgttaggt gatgcaacaa agggatattt taagccatat actccgaagc
     3481 tgttattctg tagcttatgc tgttctttat agctctggag aacggaacaa cggtattcaa
     3541 tactttatca ccaaggttct ttttcattat ttcgagcaac tcacgatctg ctttgttgcc
     3601 tttatcatat ttcgatgcga agcaagcata ttttgttccg gcatttgcga ttttttgtgt
     3661 tacccttgca agtccctgaa ccgcaaatgt cccaagctca atagggacat agacataatc
     3721 acatactgtt agaacgtgtt ctgtaaagtc gttcattgcc ggcggcatat ctactattat
     3781 gtagtcataa tccgactgcc gaaattttat tatatttgtt tgcttttctt catcggacaa
     3841 gttggcgaaa tcatttgata ttgagttcat acgcaccgat gacgtgacta tatcaatgtt
     3901 gtcataacgt gtgcttttga tcagcggcat ttcttccgca ttgacgatgg cactttcaca
     3961 gccacattca tcatttgcta agtcagtgaa aaaacgtgag ctgttgcact gtccgtcaaa
     4021 gtcaaccaaa agaatacgat tttgacgacg ggatagtgta taagctatgt tgatcgccgt
     4081 ggaagttttt ccgactccgc ctttgttgtt gtaaaatgca atcattttca taacagtaaa
     4141 cctctttctg ctgcataaag cagctacaaa attaagcccc tttttaaaaa ataataagca
     4201 ccgggaaagg acggcaggct tacttttccg tcctcgatgt agctatcatc gcccgatgca
     4261 cttcggaaca caaatcattc ccatcataac actaacttgt gttccgaccg ttgagagccg
     4321 gcagagttgc actgccgcag gttgcttttg ctctcatata ggtgcgacac cgtatcgcac
     4381 aggacgttcc tgtcgcacca tattgttcta ttttcttgtg tcatagcctt ttttcttttc
     4441 ttcttcaagc attgcaacat actgtccgta actcatcttc ttatcgagag ccatcttctc
     4501 gacttctgat attctgattt cactttcggt ttttcgtttg tgcggcgcag taaaatcaat
     4561 tatctttgca ggcatcagct cacctccttt ttaaaagcag aaagcacgga cgagccgtgc
     4621 tttataatat ttgataaaca tctcaaatta tgatataata atcaaattaa ttggaaatgg
     4681 agaggattta atgaataata acaaaaatat cactgtaaaa caggcgatag atcgctatat
     4741 agaaagcaaa accgctgttt tatccccatc tactatctac ggttacaaaa aagcaaatca
     4801 gcgttatttg tatattttgc acgacgttaa gatagctaaa cttgaccagg aacttattca
     4861 gcgagcaatc aataaattat cttattgtta ttcacctaaa acggtacgta atgcgtatgg
     4921 tttattgcac gcctctttgc aaatgttcgc tcccgatttt gtctatcgta tatcactacc
     4981 gcaaaagata aaatcatcat tttatgtacc ggatgaaaac gaaataaaag taatatacaa
     5041 actaatcaaa aatacagagc ttgagattcc atttttatta gccagtcaat gtggattgcg
     5101 tccttcgaaa atatgtgcat tgacaaagga ttgcattacc gctgacggat tagaaattcg
     5161 caaagcagtt gtaataaatg agcatggcag agaagtatat aagccgccta agtcatacgc
     5221 cggatatagg acaataccaa tatcgccctt actgcgagat aaactcatga atactccgga
     5281 taaaaaacgg atttgcgtta tatcttcaaa taaaataagc cgtgattgga attcatttct
     5341 tcgtaaacac gggctacata tatttagatt ctattctttg cgacattatt acgccagtca
     5401 agcattattg cttggaatac cacagaaata tattgcggaa ttaatggggc attcatcgac
     5461 tgacatgatt gaaaaggtct atcagcacac ctttccatct gcgatcgaag aatttaaggt
     5521 tcgtattgct gataacatag aaaaactttt ggggtaataa gggttcgaat ccctcttggc
     5581 gtgccaaccg ctttgattgc caaagcggtt tttcttttgc atattatata cagaaaaggc
     5641 ggaacggttt tttatgctgt tccgccttat attattccgt tttaacaaat tcagataccc
     5701 attggatccc ccagaacacc gtaagatgaa tagccgggtc agacagcata agtaccgcac
     5761 gctccgtttt acttgaatca tcgtcctgca ctctgagtat ctgataagca atacccgcaa
     5821 ttccgatgaa cacggcagga agcatacttg tggcgaagct cattgcaacg ccgtataaaa
     5881 caaccagtcc gcatgtccct ataagccttt ttaaaaagta acgtgacgta tcgacctttc
     5941 cacagagtgt tataattatt gcaattacac tacatattat agctccgaag caaacatacc
     6001 gccacaaagc gtcaagccgt gtaaacgctg aaattccgcc cgcataaaga aaatcggtta
     6061 ccgttcgtgc aatcccccat ataataatta ttaccggaaa aaacagtgat tgtaaaagcg
     6121 ccgttctgtc atctttaaac ataatacacc tcgtttgtca aaagtacatc ttgaacgatg
     6181 ccgtttatca cttatgatac cttttccccg caataatact atacgccctg tagatctgct
     6241 ccagaagcat cacccttgca agctgatgcg gaaaagtcat cttcgacata gacagcttta
     6301 tatcagccat cgcctttatc ttgggagcaa gcccgaacga gcttccgatt atgaagttca
     6361 ccgccgactt tccctgcacc gccgtatcgc tcagcttagc tgcaagctcc tcgcttgaaa
     6421 gctgctttcc ctcaatgcac atagcaa
//
LOCUS       DAWAOG010000043         6384 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1672_length_6384_cov_2.166535, whole genome shotgun sequence.
ACCESSION   DAWAOG010000043 DAWAOG010000000
VERSION     DAWAOG010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1672_length_6384_cov_2.166535"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1156..1165
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5391..5400
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1976 a         1299 c         1616 g         1473 t
ORIGIN      
        1 attcaacagt gatggcggta ttctttgata catcgacatc agccgacgaa acaatggacg
       61 ctatcaacac gataagagat gtaacgaaca atcagtgttt cgtttccggt atgtccgcaa
      121 tggtaacaga cctcaagaac ctttgtgagc aggaagagcc gatatatgtc gctatcgcag
      181 tagttcttgc ggtagtagta atgatgctct ttatggattc gttcctgata ccgatcatct
      241 tcctcgcaag tatcggtatg gctattctct acaacctggg cagtaactac ttcttaggcg
      301 agataagcta cataacaaag gcactttccg ctatcttgca gcttggtgtt acaatggact
      361 actcgatatt cctctggcac agctataacg agcagaaggt aagatttgac ggcgataagg
      421 aacgtgcgat ggcacacgcc atttcaaaca gtatcatttc tgtagcaagc tcgtcgatca
      481 caacaattgc cggcttcctt tcaatgtgct ttatgagctt cacgcttggt gcggaccttg
      541 gtatagtaat ggctaaaggc gttgttttcg gacttatcgg ctgcgtaaca attcttccgt
      601 cacttatcct tgtattcgat aaggctatcg aaaagacaag acacaaacag cttatgccga
      661 agtttgacaa ggtggcaagg tttataacaa aacgttcatg gatattcctt ataatattcg
      721 ctgtacttat cggaccagcg gtttacggtt ataacaacac acagaaatat tacgatctga
      781 gccagacaat gcctcaggat atggacttcg ttatcgccaa cacaaagctg aaggacgatt
      841 tcggaatgtc gaacgttcat atgatactcg cagattcaaa gctgtcacag aaggacgcaa
      901 aggcgatgct tgatgaggtc ggaaaggttg acggtgttaa tcttgcagtc ggatttgatt
      961 cgcttatcgg ctcggctgtt ccctcagaga tgatccccga ttcaatatcc gaagtgctta
     1021 agggtgataa gtatcagctt atgcttgtag gcagtgagta cggtacggca acagacgaag
     1081 tcaataatca ggtaacagag ataaacaaca ttataaagaa gtatgacagc tcggcaatgc
     1141 ttatcggtga agctcnnnnn nnnnncgaca gggacttcaa tgtagttgac gttatttcaa
     1201 tagccgcaat cttccttatc attctggtaa ccttcaagtc ggtttcactc ccgctgatac
     1261 ttgtggcggt aatcgagttt gcaatcttcg taaacctcgg tattccttgt tacacaggta
     1321 cagagcttgc attcatcgca cctatctgta tcagtactat ccagctcggt gctaccgttg
     1381 actatgcgat cctgatgact acaagatata aaaaggaacg taataagggt aacgacaaga
     1441 atgaagcggt ttacacggct cttaaatctt caataccttc gatcatcgta agtgcgctcg
     1501 gattttttgc cgcaacattc ggtgtaggta tttattcaaa tgtagatatg ataagctcgc
     1561 tgtgtgctct tatggcaaga ggcgctatca tcagtatggt atcggtaatc ctgttattac
     1621 ccgctatgct caagctcttc gataagataa taatccacac gagccttggc ttcaaggaag
     1681 caagagaagc cgataagaaa acgaactgag aaaggacaac agcatgaaag agataagaaa
     1741 aaagatcata aagataattt caatagtact tgcagccgca atgataggca catcctcggg
     1801 tctgcttgca ttcgccgcac agagcggtaa cgatagcagt acatcgtcat cggtttcgga
     1861 cagcacggaa accggcagca gcgaaaagag cagctctgat acggacggca aggaagaaac
     1921 ggtatatatc attgcaaacg cagatggtac aacaaagaag gtcatcgtca gcgactggct
     1981 gaagaacggc tcacagagtg catcaatttc cgatgaaacc tcacttaaag atataaagaa
     2041 cgtcaagggt aacgagagct tcacaaacaa cggtgaaaac tacacatggg ctgccaacgg
     2101 tagcgatatc tactaccagg gcacaactga caaggcactt cccgttgata tgaagattac
     2161 atatactctg gacggcaagg cagtaactcc cgatgagata aagggcaaga gtggcaaagt
     2221 cacgatccgc tttgactata caaataatga aaagcgtacc gtaaaggttg acggcaagga
     2281 tatggaaatg tacgttccgt tccttatggt gacgggaaca gttctggata acgagaagtt
     2341 cacgaacatt aaggtcacaa acggtaagct tgtaaacgat ggcgacagat ccgcagttct
     2401 cggttttgca cttccgggta tgcaggaaaa cttaggactt gacaaggatg attatgaaat
     2461 cccctcatat gttgaaatca gcgcagatgt taagaatttc gagcttatga cctgcatgac
     2521 ggtaggtacg acagagctgt tcaacgaggt cgataccgat aagctcaata tggacgatat
     2581 agacggcact atcaaggaat ttacagacgc tgtagatcag ctttcagacg gctcttcaca
     2641 gctttatgac gggcttgtga cattatttga gaagtcaggt gagctagcgg acggcacaaa
     2701 gcagctcgca gacggtgcag gcacacttta tgacggaaca ggaacacttg taagcggtgt
     2761 aaatgatctt aaaacaggcg cagacgcgct gaatacgggt gcggtacagt taaaggacgg
     2821 tacggtttct ctggtagccg gcatcaagga tctgaagtca ggtgcggaag cacttgacag
     2881 cggtgcggga caattaaagg acggcacagg tacacttgta agtggcgtta atgacttaaa
     2941 gagcggcgca gacgcacttg acagcggtgc aggacagtta aaggacggta cgggaacgct
     3001 tgtaagcggt gcaaatgacc ttaaagacgg tgcgggcgcg cttgacagcg gcgcgggaca
     3061 gctaaaggac ggtacaggtt cacttgtaag cggtgtaaat gaccttaaga caggcgcaga
     3121 tgcactgaat acaggtgcag gacagctgaa ggacggtaca acagctcttg taagcggcgc
     3181 aaaggatctt atagccggcg cagatcagct taacagcgga gctgcttcac tcgacagcgg
     3241 cgcggcacag gttgaaagcg gcgcaggaca ggtaagcacg ggagcctctg atctcagcaa
     3301 gggactagga cagctttctt caaacagcga acagcttaat gcaggtgcaa agcaggtttt
     3361 tgaaacgtta ttatcaactg ccgaaacaca gataaaggca tcgggtctta ctgttaatac
     3421 gcttacgata aagaactaca agacagagct caacaagctt gtaagctcac ttgataaaga
     3481 taaggtatac gaccttgcat ataatacggc tcttaaaaag gttacagctg aggttgaaaa
     3541 aaataacgat gctataacag caggtgttac tcaggctgta caggcaaagg ttcttgaggc
     3601 ggttctcaag acagcaggtt atgagatgac cgctgagcag tacaatgccg cagtaaaggc
     3661 aggacaaatc ccggaagcgg tacagaagca ggtcgatgcc gcagtaacgg cacagatggc
     3721 aacagacaat gtaaaggctc agataagcac gaatgttgaa gctcagaaga aacagcttat
     3781 tgatcagaat atgaagagtg acacagttac aactcagata aatgaagcgg ttgcaaaggc
     3841 tgcggcaggt cagacaacga taaagaacct tatcactcag cttgactcct acaatcagtt
     3901 ctatacaggt cttacaacat atacagccgg tgttgacagt gcatacagcg gttcacagac
     3961 gttgacagca ggtgcatcac agctttacag cggtactaag gatcttcaca gcggtgctac
     4021 tcagctcaag aacggcactg agcaactcac aagcggcggc tcgacactta tagcaggtgt
     4081 aaatcagctt gacaagggag cgggcgactt aaaggacggt acggcaagcc ttgtcacagg
     4141 tgtcgataca cttcacagcg gcgcattaca gcttaataaa ggcgcaagcg atttgaagga
     4201 cggtacagca agccttgtga caggcgtagg cacactgcag agcggtgcaa ctgacctcaa
     4261 taagggcgca agcgatctga aagacggcac agcaagcctt gtagcaggtg taaacacact
     4321 ttacgatggt gctgttcagc ttgataacgg cgcaggtgat ttaaaggacg gtacagtaag
     4381 ccttgtagca ggtgtaggca cacttcacag cggtgcgtta cagcttcatg acggcgcagg
     4441 cgaccttaag gacggcgctg ataagcttaa tgacggtgta gcaactctta ttgacggtgt
     4501 aaagcagtta agggacggtg ctaaggaact gaatgacggt atggacgagt tcaatgaaaa
     4561 ggctgtcaag aagatagttg acgctgttga cggcgatatt gcgggactta tcgacaaact
     4621 gaaggctaca gtagccgcag gcaaggacta tgacacattc ggcggtaagt cggataagac
     4681 agaaggcact gtaaagttca tttacagaac agagagcatc tcagccgacg atgaataatc
     4741 aataacttaa taagtcaaag gctctgacgg gggtaacgct ccgtcagagc ttttttatta
     4801 caaatgtcat acaggaaaat atcgtttgta actggtgaaa tccgctttgc aggtgtataa
     4861 tcgttacagt aagataaata aacagccgaa aggggaatac ttatgatatt tgcaagcgta
     4921 aaaaatctag tcaagaacta tggcaatcat tgcgccgtag ataatctgag ctttgatgta
     4981 aatgagggtg agatattcgg acttataggt cctaacggag caggaaaatc cactacaatc
     5041 aatataatta caacccttga tgaaatgaca tcgggcagtg tgatgataga cggtaaggat
     5101 attgcaaagc aaagagcgga aataaggcgc ataataggca gtgtaccgca ggaaatagcc
     5161 gtgtatccat atcttacagc taaggaaaac gttgaatttt tcgcttcgct gtacggcttc
     5221 aagggcgagg agcttcataa gaatgcaatg actgcgcttg agtttgccgg gcttgccgat
     5281 aaagcggata tgaagccgaa gaaaatgtcg ggcggtatga agcgcagact gaatattgcc
     5341 tgcggtatag cgcacagacc gaagctgata gttatggacg agcctacagt nnnnnnnnnn
     5401 cagtaggagt tgatgcacaa tcaagagagc atataatgag ttcgataaag aagctgcgtg
     5461 aaagcggagc gacagtaata tatacctcgc actatatgca cgaggtagag gaaatctgtg
     5521 acagagtcgc tataatcgac aaaggtaggc ttgtggcaga gggtacacag caggagctta
     5581 taacaatgat aaccgatagc tgtacggtac atatcactac aaaaccctgt gacagcgaaa
     5641 caagaagcag agtgtcaagg gcaatatcca atcttcctga tgtcaacaga gtataccttg
     5701 aagaaaatgt gataagtgtt gatatgagta tcagctgtaa cgatatttca cacgttataa
     5761 gcgagcttgt aaagcacgat ctgcctgtaa tcgagataag cacggaaaag ccggatatgg
     5821 acgctgtgtt cctgtcgctt acaggacacg agatacgtta aggagcggaa atatgcttag
     5881 tttgataata aaacagatta gggagtattt cagaagcccc gcaaatgtat tcatggggct
     5941 tttcttcccg gtactgctgg tattctttct cggaacgatg cttcagaatt ttgatatagc
     6001 ggattatgac ataggagaaa ttaaactgca atacaatatt tcgcaaacag acgaacaaag
     6061 cggcaaggca tttgctgagt ttcttgacaa tatcagtatg ataagggcag aaaaggcaag
     6121 cgatgaaaaa accgcactga gccttaccga taagggtgat atagacgcat atatcgagct
     6181 tgaaaacggg gaaattctgt tatacagcgg cagaaacgaa attgcaaaca gggcacttgg
     6241 ctcggtgctg aattcttata ttgcggtatc cgatacatat tataaaatag cggcaaccga
     6301 cccgtcactg cttatgaata cagataccga taccggaaag tcgtttgtag agcaggacgg
     6361 acttggcgtt acacggagta tgat
//
LOCUS       DAWAOG010000044         6349 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1680_length_6349_cov_2.188275, whole genome shotgun sequence.
ACCESSION   DAWAOG010000044 DAWAOG010000000
VERSION     DAWAOG010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1680_length_6349_cov_2.188275"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1470..1479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2719..2728
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3827..3836
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1802 a         1493 c         1473 g         1551 t
ORIGIN      
        1 atcaacgacc attacggtta tgccgcaggt gattgtgtgc tgaaagcggc ctgcgactgt
       61 atcaaaaact gtataagaag cagtgatatt cttatacgtc ttggcggtga tgagtttgtg
      121 cttctgatga ccaatatccc cgaaattata ttcagacaga agctcaatga aataaagcaa
      181 agcatctcgg caataaaact caaagattat ccggatatta aatgcgctgc cgctatcggc
      241 ggtgtgtacg gcatacagcc gatagagaaa gcgttagccg aggcggataa gctgatgtat
      301 ttggataaaa acgcctcaaa ggagtataaa tgacacttga agaatgctat aaggcgctcg
      361 gaggaaacta tgccgatgtg ctttcaagac ttacaaatga taagatgata acaaaatatc
      421 tcggcaagtt caccgcagat acaagcttca atgatatttt cactgcactt gacagcaaag
      481 attatgaagc tgccttttgt gccgctcata cgctgaaagg tctttgtctt aacctcggga
      541 ttgaaaagct gtatagatcc gcatacaagg taaccgaggc gcttcgcaat aaaacagacg
      601 aaacgactcc cgaaatgctt gacgaaatga aatcaaatta caaaagcgct atccttgcga
      661 taaaacagct ttaattacag atccctgcca cacttcctca ctaaagtgtg gctttttact
      721 tgacgatgac cgcaaaaaaa ggtataatat tttcagacct ctgacccttt tgccttttgg
      781 caaaagacgg agcactgccg tttcgggcgg tgtcggtata atttacggta ttcagatata
      841 aaccggaatg acaaatccgc tttttaagaa agggacaaac aatgacagta agagaaatgc
      901 aggacgagat actgagaata aagaaagaaa aagatatatg cattctggca cacgcatatc
      961 agcgtcagga tatacttgat gttgccgatt tcgtaggtga ttcatttgca ctcagccgga
     1021 tggcggcaaa agcaccgcag agcacagtgc ttatgtgcgg agtgcgcttt atggcagaaa
     1081 cggtcaaaat actatctccg caaaagaaag taattttatc cgacagcaat gcgggttgcc
     1141 ctatggccga acaaatggat aaggagctca tctcacagct taaggaaatg tatcccgact
     1201 acaaggtagt agcttatata aatacgacct cagagcttaa gacagtgtgc gatgtatgcg
     1261 ttacttcatc aagtgcggtg aagatagtca aaaagcttga cagtgacaag atactcttca
     1321 tacccgactg caatctcggc gactttgttc agaagcagtg ccccgataag acgttcaagc
     1381 ttgtaagcgg cggctgtccc acccatctgc gtatgacggt caaagatgtt gaaaaggcaa
     1441 aggctcagca ccctgacgct ctactgctcn nnnnnnnnng aaaacagcat tgtcgaacac
     1501 ttaagctttg acgaacccgg caaaaagttc tacccattat caaaggattg cgtatgtcac
     1561 aatatgcacc ttacaaacct tgcaacggtt tacaactgtg tatgcggcac agcaggcgag
     1621 gtcatagagc ttgacgagga cgtaaggctc ggcgcgaaac gttgtataga cgaaatgtta
     1681 agactcggag attaacgacc gatatacggt ttataataaa actgtaataa ccacgtccgc
     1741 ttgacttttt acgcgacgtg aattataata gaataagcag aaatttccgc ccgaaaaggc
     1801 ggtgtttttg caaaaggcgt aactgtttac aatcgaaagg aataaaaatt tgaatattaa
     1861 aagaaaatta gcggcgatcc tcgcaggaat aacagcaata acagcattct caggctgttc
     1921 tatgctcggc ggtcagacaa caaacgcact cggaagctat gattttacag aaatgaatct
     1981 tgttcagctt gacaagcccg cagacggtca ggacgttgca gtaatagaaa ccaatgtcgg
     2041 tacaataaca gcggtactct atgccgagtt tgcccctaat acggtagcaa actttaagaa
     2101 gcgtgcggag gaaggcttct atgacggaaa gccgttcttc gctttgcaaa agggcattta
     2161 tgcaataaca ggcgcttcaa acgatgaagg tacggaaggc ataaccgatg acggcaagta
     2221 catcgaaaac gaatgctcag taaacctgtg gcctttcaag ggtgcgcttt taggatacag
     2281 cacacagcag ggctattcag acagccgttt cttctttaca ggcgctcttg agataaccga
     2341 agaaaacaaa aaggaactca gaggctatac aaatcaggaa acaagtgctc aggtaatacc
     2401 ggaagagctt ataacggcat ttgaaaaaag aggaagcgtt cccggttttg caggtgctta
     2461 caccgtattc ggtcaggtga taaacggctt tgacgtgctt gataagatac tctggaccga
     2521 ttcggacaag tcaaacatga agccgcttga agatctgaaa atagtaaaag taacgctcga
     2581 tacctataag gatggaaagt tcgcaataga agagccgaca tcggataagt atctttctgc
     2641 cgaagaaatt gctaaaatcc agaacgaatc aaacagcacg acaagcagtg taaccgagga
     2701 aacatcggca aactgatann nnnnnnnntg ataacaaatc gtaacggtga aaggaaccga
     2761 taaaatgagg tttaagtctt tattcaaggc tgcggcggcg gtaacgctgg cagcagctat
     2821 aataacagca agcggctgta gctcacagaa aaaggataac accgcagaga taactgtagg
     2881 tccgggcgac acacttgccg aaatgacaat acagataggc gaggaaacag gcaagatacg
     2941 cttcaagctt ttcccggagg tcgctcctaa gggcgttgac aactttgtaa agcttgctca
     3001 aagcggttat tatgacggca agactatcca cagagttatc aaggacttta tggtacaggg
     3061 cggctcactc agaggaaacg gcacaggcgg aactaccgcc gataagaccg caattccgcg
     3121 tgaaactaac gaccgcatga gagcgtatta cggagcactc ggatatgccg aaggcagtga
     3181 cggcatcaac agccagttct ttataataaa taacaagaca cctttcgaca tagcaaaaac
     3241 cgccgagaat atctcgtcag accttaacga atacaaggac agactgacgg aagatgacaa
     3301 aaagacatac accgactacc tttcaaagct caatgcgacc cccgatgatg taagggcaaa
     3361 atacctctca aacggcggtg tattcagcat cgacggaaac tacacaatat tcgggcaagc
     3421 tattgaggga cttgattttc ttgacaagat aagcgctgtt gaggtttctt caggaaacac
     3481 aatagacgat gaacaaggta ttgcgtcaaa gcccgccgtt tcggtgataa taaagaaaat
     3541 cgagattgcg actatcccga caataaccga aaccgaggct actaccacaa caagaaggag
     3601 aatcagacct tctgccgcaa cgacccccgc agaggtcacg acagcggctg aaactcccgc
     3661 tccccagtcc tcaacaacca agtcggaata agtacatagc gtaatatgac agacgaacgc
     3721 gccccctgat ttgaatgtaa atcagggagg cgcgttttgc tttgtaagct ccgcgtaaaa
     3781 gcagtctgca agcggttctg acggctttat ataagaagca ggtcgtnnnn nnnnnncaca
     3841 ttccgcacat cattccgtcg atcttaagtg ttgtctttac cattgtaaat tcctccgtta
     3901 ctttattgat attactttat agccgcagtt ctcaacggct tttttaagct gtttgtcagg
     3961 gacttcgccc gatagctctg ctatggcgat accgtctgtg tggctcgctt tggcagatat
     4021 tacctgaggt attgcttcaa gtgctgtctt tacatgatgc tcacacttct cgcacatcat
     4081 accttttact ctgatcgtcc tggtaatatt tgtgagattt ttggtttgta ccaccggtac
     4141 attttggtac actatcggtt tcgcctttct gtcatacttg taaatcttac agaaattgag
     4201 tcttagagca tttgaaacaa cgcagaagct tgacaagctc atcgccgccg caccgaacat
     4261 aggattaagt gtaagtccta aggaaacgaa cacacctgcg gcaagaggta tgccgataat
     4321 gttatagata aatgcccaga acagattttc gtgaatgtta cgcagtgacg cgcgggaaag
     4381 tcttattgcc gcgggtacat cggagagcct gctcttcatc aatacgacat ctgccgcatc
     4441 tatcgctacg tctgttcctg cgcctatggc gatacctgta tctgcggcgg taagtgcggg
     4501 agcatcgttt atgccgtcgc ctaccatggc gaccttaccc tgagctttaa gcgaacggat
     4561 aacagcctcc ttgccgtcgg ggagaacacc tgcgatgacc tcatcaacgc ctgctttatc
     4621 tccgatagcc cttgcggtgc gctcgttgtc gcctgtcagc ataacgacct ttatgcccat
     4681 atttctgagc tgacgtatag catcggggct gtcttccttt atcgtatcgg ctaccgcgat
     4741 cataccgagc gccttaccgt ttctactgaa caggagcgga gtcttgccct cgtttgaaag
     4801 gcgctctgct tcggcggtta ttgtatctga aagcttggca ttactgccga tgaacttaag
     4861 actgccgccg tagagggttt cgcctgaaac cgctgcggta agtccgttac cgctgagcgc
     4921 cttgaaattc tcaacattct gtgccttaat gccgtgctcg ctgccgtata cgtttattgc
     4981 ctttgcaagc gggtgctcac tctttgcttc aagcgataca gcaaggctta aaagctcgct
     5041 ttcggtcacg ttgtctgcgg ggataatatc cgttacctgg ggttcgccct ttgtgatagt
     5101 tcctgtcttg tcgagggcta ctatctgagt tttgcccgcc tgttcaagcg aaacggcggt
     5161 cttgaacaga atgccgttct ttgctccgac accgtttccg accatgatcg caacgggggt
     5221 cgcaagaccg agtgcacacg gacagcttat taccagcaca gaaattcctc ttgcaagggc
     5281 gtatgcaaaa tcctgaccga ggagcagcca tactatcatt gtaattattg aaattgtgat
     5341 aacggcaggt acgaatatac ccgatacctt gtcggctatc tttgcgatag gcgctttggt
     5401 agccgccgca tcgctcacca ttctgatgat ctgcgacagg gtcgtatctt cgccgaccct
     5461 tgatgcctta caggtgatga agcctgtctg atttatcgtt gccgccgaaa cattatcgcc
     5521 gactgtctta tcaacgggaa tactctcgcc tgtcagagcc gactcattta ctgcgctctc
     5581 accgtctgtc actacgccgt caacgggtat gctctcgccc ggacgtacta caaaaatatc
     5641 gtcctttacg acctcttctg cggggatagt ctgctcaata ccgcttctta tcacgtttgc
     5701 ggtttttggg gcaagcttca taagaccttt gagcgcatcg gtggttttgc cctttgagcg
     5761 cgcttcaagc atcttgccga ctgtgataag cgttaaaatc atagccgcgg actcaaaata
     5821 gaactcgtcc atatacatca tcacagcgtc cgcgttgccg tgaagctgtg cgtccgtcat
     5881 cataaacagg gcatacacac tccatacaaa tgacgcaccc gagccgagcg caacgagcgt
     5941 atccatattc ggcgagcggt tgataaagcc cttgaagccg tttacaaaga acttgctgtt
     6001 tataaccatt ataatgccgc tgagaagcag ctgtacaagt cccatagcca catggtttcc
     6061 gtcaaaccat gacggaagcg gaaatcccca catcatatgt cccattgaga agtacatcag
     6121 tatcacaagg aatatgacgg aggctataag ccttttgcga agcttaggtg tttcggtgtc
     6181 acggagtgaa tcggcgctat tatccgcttt ctttgcgcct gtgcctttaa gctttgcacc
     6241 gtagcccgct ttttcgactg cggaaattat atcctgcggc ggcgctgtgc cctcaactcc
     6301 cattgaattt gtcaacaggc tgacagagca cgaggtaacg ccttttacc
//
LOCUS       DAWAOG010000045         6234 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1709_length_6234_cov_2.109403, whole genome shotgun sequence.
ACCESSION   DAWAOG010000045 DAWAOG010000000
VERSION     DAWAOG010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1709_length_6234_cov_2.109403"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1442..1451
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5525..5534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1516 a         1455 c         1267 g         1976 t
ORIGIN      
        1 tataagcacg aaactatcaa tactttttaa ttatactaca atatctgccc gattacaagc
       61 attttatttc tttgccgaca caaaaatcac tgcctgacgc ttcggtaatt tgtacagtaa
      121 ttatatcctg cttttttgcg tcactgccgt agatgcgtac agggacatat tgcggagtaa
      181 gtccgtttgc atattcctcg ctttctctgc gctgaataag caccctttcc gtttttccca
      241 catttgagcg gaagtacgct tctttaagct cgttagcaag cttgctcata gcatgatatc
      301 tctttaccgc aacatcgtgc ggaacatggt ctgtgcgctt tgccgctgca gtaccctctc
      361 ttatagagta ggtaaagacg tgcattgccg caaagccgac ttttcttaca aactcgcacg
      421 actcctcaaa ttctttgtcc gtttcgcccg caaatcctac cataatatcg gtagttatag
      481 cacagtccgg gaacgctttt ctaagcctta cgactatatc gtaatattca tcgggcgtat
      541 agtgcctgtt catacgcttt aacgtagcgg cacttcctga ctggagcgac aggtgaaaat
      601 gcgggcagag cttcttctgt gccgccattc ttgctatgtc ctcatctgtc agtagctccg
      661 gttcgagcga tgacaaccgc acacgttcga caccgtcaac agagcatacc gcctcgatcg
      721 cgtccgcaag cctcaaaccg ttatcctgac cgtagcagga aagattgatg ccgacaagca
      781 ccatttcctt atgaccgcac gaaacctgaa accttgtttc ttcgattata tcctgcaaag
      841 ggcgtgacct tacagagcct ctcgctgtgg gaattatgca gtaactgcaa aacctgtcac
      901 agccgtcctc aatctttata aacgctctcg tcttgcccat atcggcgttt ttctgctttt
      961 catattgctt tttaactggt cttggcggta tggctacctg ttttacattg ttgccggtaa
     1021 atatgtctat catatcggca agactttcct tgtgctctgt acctgttacg atatctgccg
     1081 acagcttcgc cgcagtttcg ggaaaagcct gcgggaagca gcccgtcagc acgactgtct
     1141 tcatagggtc gcggcttttt atcctattta tcagctgttt tgctttttta tcgctgtttt
     1201 ccgtcaccga acaactgttt attataacga tatctgccgt aaaaatatcg tcggttttct
     1261 tgtatccgtg ttcgtccata gcttttgcta tcaaagcgct ttcgtattgg ttgactttac
     1321 agccgaaggt ctgtacggta tatctgataa tatcacttcc ataataaatt gtggtaagtt
     1381 tcactaaaag taaactcccg actgcttaat tatactttat tattacgaat tttgcaagtt
     1441 gnnnnnnnnn ntgacaataa tactcttgac cgctgtgtga aatgtgctat tatatagttg
     1501 taagaagcga taagctctta cataattaag gcggaaaaag ggaaatcagg ctaacggtca
     1561 cctctccgat tgaaaaattt aagtaaattc agtttaaaag caattgacaa ccgccttaca
     1621 ttttggtaaa atgaaatcaa tcggacagga aagccgaaaa atgaaaggta ggttttttgt
     1681 atgaaaacag ctaatatcag aatcaatacc attgaagacg ttaagaattt cgtaactaca
     1741 gttacaaagt gcaattacga tgttgatatc gtaagcggca gatatgcaat tgacgctaag
     1801 tcgataatgg gtatcttcag cttagatctt tcaaagcctc ttgagcttcg tgtgcacagc
     1861 gatgactgcg atgaacttat ggctgagctt gataagtata tcatcaagta atctttaatt
     1921 cttaatagca agagccgccg tgcagatttg cacggcggct taattttgtc ttattcagtt
     1981 acggttattg agtttgcgta ctttatcttg cagagaatgg tgtttttatc accctccaac
     2041 tttccgatac ggcttccgtt agaatagatt atatcgccgt tttcaagaag tctgtaatcc
     2101 attatacgct tttttataac agtttcgttt ccgctcttgt cacgtttaac aagctcccag
     2161 tcggacggga taacaccgtc gtccgcgttg ccgttaagct gtttttcagc ctttatcata
     2221 ttgccctcga taaacgcttc acgctcatcg gcagtcttac tctttgcggg attttttcca
     2281 ccggtgcgca aaggttctcc gccgaacgcc attgagaata cgcttaaaaa tccacctata
     2341 gccttgatta ttctgacagg gaacataagt atgtctataa gcaggttact tttcggtttt
     2401 gatgaaacgt attttctgcg tatgtagtat atattgccgt cagcgtcatc ctgtggcttt
     2461 atgcagtcat atccgtcctc ggctatatac tcatctatag taccgctccg ggtatcgtaa
     2521 agacatatcg tgcacggact tttttttgca acatttccgc ttctgtctgt tgcaaacccc
     2581 atagcagtat agtagattat atcgctgttc ctgcgtgaaa taaacgggtg agtttcgctg
     2641 gtgaaccctt ctgtaagctg ttcaaagctg ccgttgtcgg tgcttacaag cgcgatgtga
     2701 ttttcataat atccgtttcc cgcactgaca acgcattttc cgtcatagca gtcaatatca
     2761 ttgattattt cttcgcggtt tgcaaatatg tagttttctc catcgccgtc tttgaaatac
     2821 ataccgccga cattatccgc aaaggtagaa tatatcagct tgtcgccgca agacccgatg
     2881 cctctgagca tactgtacgt ttcttcctcg ttgtattcct ttggctcaac gcccataaat
     2941 ttagcaccct tgccggtggt tttccattcg tcgcgggctt tcatcttacg gatcacgtca
     3001 cggtagcttt ccacacgctc gcaaggaaca cggcgttcct tgccgttttc aatattataa
     3061 agttcgcctt ccgataggca gtaaatattc atattatccc cctgaagccg ttatttctgt
     3121 ccgtaatatg cgttagcgcc gtgctttctc agatagtgct tatcaagaag ctcctgctgc
     3181 ataggctcga tattcttgtc aagcaacaga ttgaacatag ccatctttgc cacgttttca
     3241 agtacgaccg cattgtgtac ggcttctgtt gcatccttgc cccacgcaaa cggaccgtga
     3301 gagtgcacgt ttacagcagg tacggcatcg ggatttatat tacgcttttt gaatgtgtca
     3361 attattattg tgccggtttc cttttcgtat tcgccgccga tagcttcggg tgtcatcttg
     3421 tctgtgcagg gtatagcacc gtaaaaatag tcgccgtgcg ttgtgccgag cgcgggaata
     3481 tcaagtccgc tctgtgcaaa ggtcgtactc cactgtgaat gtgtgtggac aataccgccg
     3541 atgttaggga atgccttgta aagttcaaga tgtgtcggtg tgtcgcttga cggtcttaaa
     3601 ttaccttcga cctgttcgcc gtcaagagtt acaactacaa ggtcatcgac cgtcataccg
     3661 tcatattcga cgcctgaggg tttaattacg acaagtcctc tttcacggtc gattcctgaa
     3721 acgttgcccc atgtgaatgt tataaggccg tatttcggga gcaaaaggtt agcatcaagc
     3781 acttgttttt ttaatttctt taacatattc ttgtttctcc tgtttttatc atttgcattt
     3841 tttaatgaac ctaccgtcca ttttctttat gaaataattt cttacattca gcgtttcctg
     3901 cggggtcagc tgatttttcg gcagacagaa cgcaaggtca tacctgaaat atatgataaa
     3961 acaattttcg atctcatata tcttgaaaat tgtgttccat tgtgcgttta tatccgttgc
     4021 tgttcttccg ccgaaaactt ttaccgatgc ggcatcgtaa ctgaaagtgc gctttgcgtt
     4081 gagtgctacc ttgccgtact ttatgccgtc cttagctttc ttggatataa gaagaatatc
     4141 ggcaaggatt accgcaacac aaataataca tacagcagcc gaaacataca gcgggatcat
     4201 tcccgcaagc ccgataacga gcgatgccac cgcaagtaca gccatagcga tgaacagaat
     4261 gttattggat tttttaagcg aatattccgt gaattttacc aatgtgcggt aatcgtccgc
     4321 agtagttgtt atttccttag aaaacattta ttattcttct ttctgatttt catttgtttc
     4381 agcttttttc tttcttacaa agctgtcatt ctgccttttc ggttcggtgt gtttgtcgct
     4441 ttccgcttta gtcgcgggtt caattttttc atcaaaaact ttatatatct cgtccttttt
     4501 ctggacaaac agaagctcat cttcacctgt aaccacatcg accttttcac cggactcaat
     4561 atcggtatgg tacacggata tcgcgtcaaa acggtagctt atcccctcgt attttacttt
     4621 tcccttgccg tagccgttcc cgcctatatt ctcaagcgcg gtggcttcat atccggtgag
     4681 gtcatcgggc ttcggcgctt taccgtttac aaagcgcttt acggcggggg agagaaaatg
     4741 tctaccgatg aagttcacga taaatgccat agctatatcg gcaggtacga taaagtacca
     4801 cggcagcgac agaggcagca gtaacagtcc gaaatatccg aacacagccg taaataccgt
     4861 atagccgtat attccttgcg gaataaactg ctcgatcggt atcgtcctgc ccttcggcag
     4921 tctgaagccg agatttattt tcagtctgaa gaacagcagc attacaagaa gtccgcatat
     4981 cccgacagta cccagtacgg cgcagactat aaacagtaca cgcatattat tccttatttt
     5041 cggtcttcgg aattacggta aggcgtacct ttactattct tctttcgctg actttaagaa
     5101 ccgttatcct gaaatcgtct gttgtgacac tttcgtttgt ttcgggtatt tttccgaaaa
     5161 gttcaagcac ccagccgccg actgtctttg cgttaccgtc gattttagga ttatctcccg
     5221 tgcggttatg ccagtagcgc ttgaaagcgg taagcgatac atcgccgttg acattgaact
     5281 cgttttcgga aacgaatgta acaggtgcga ctatctcatc attttcgtcc catatttcgc
     5341 cgacaagctg ttcaagtata tcttcaagcg taactatacc gagcgttccg ccgtactggt
     5401 cgagtacgac agccatatgc accttgtcct tctgcatctg tttcattata tcgcttatct
     5461 tcatcagatg aggaacatag tgaatctcct gcattatatc acgcagtaag aatgtttcat
     5521 catcnnnnnn nnnntaagat atttcttgaa aaagtccttc tggctgacaa agccgataat
     5581 gtggtcgata gagccttcgt aaatgggaag ccttgaatat tcctcgttga tgaaggtttc
     5641 ctttaccgtt tcgatatctt cgtttacatc aacagctatt acatcgacac ggtgagtgat
     5701 aatctcatcg actaccgttt cgtcaaattc aagtgctgat tttacaaggt cacgctcctg
     5761 ctcctcaagt acgccctcgt cctcgatctc atcgattata gccattagct cctgctctgt
     5821 tacagtaacc tcttttttat ttccgaacag cttgtttgca agctttttaa gcagtataaa
     5881 taaagctgac aggggagtga atataaatgt aaggaatgtg attattccgc tcgttgcgac
     5941 tgtcattcct tctgagtgat cttttgcaag gcttttcggc attacctcgc cgaatgtgag
     6001 tatcacaagc gtcagtacca ctgtcgatac gagcgccgca ttgtcaggac ctactattgc
     6061 tgtcgcaagt atagtaccga gtgaagatgc ggttatgttt actatgttgt tgccgataag
     6121 tattgttgtg ataaccttat cgtatttgtc aagcagcttg agtgccattt tagcgccctt
     6181 ggagccgttt tcagccatgc ttttcagtcg tatgcgatta gcaccggtaa gtgc
//
LOCUS       DAWAOG010000046         6212 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1714_length_6212_cov_2.098749, whole genome shotgun sequence.
ACCESSION   DAWAOG010000046 DAWAOG010000000
VERSION     DAWAOG010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6212)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1714_length_6212_cov_2.098749"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2782..2791
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3149..3158
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5731..5740
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1827 a         1261 c         1464 g         1630 t
ORIGIN      
        1 gaatatggcg atgcttatca tcatctgcat tgtctgcttt gtatgtgtgc tttgctgtta
       61 caagaaattc agggcgctgc cgctgattat aatgtggtgc gtaatgttta tccccgcttt
      121 tctttcggaa gacgcggatt attcggtata taccctgctt gcggtagccg catttttcgg
      181 gctttatgct atagcgtcat cgaacagctt ttattcaaaa atacctgcgg tcggcgctaa
      241 aagaaacgaa gtaaaaaatg atgaaaaggc tttccgcaaa aatctcagca aaaaaaatcc
      301 tgttcagaca gcaaagtccg agctttcccg ctacggaaga aacagcctgt gcggaatact
      361 tgcggcattg ataacattcg gagcggcatt cggctcacag aagcttttcc cggatatgac
      421 ttacatcgat accgaagagg tcatcaattc cacagtgaaa ttctttaccg atatcggcga
      481 atatttcagt cttgtttttt ccggcaacac aagcggactt ttcagcggat atttttcatc
      541 cgacaacttc tttatcaata acaatataga actcaatgcg cctccgcagt cggcaaatga
      601 tcctgtgctc aaggtcacat cttccggtga gaacggtatg taccttgtcg gtgatatcgg
      661 tgtggacttt gacggtcgga gctggatcgg cgttcagcga aaaatatcgc agaacgagct
      721 ttacagcggt gaatataata taagtgacag cttttcaccc gagcagatcg ttcagatgac
      781 gcgctatctt aatatgtccg gtttactgtt ggaaaagaac ctgtcggatc acgaatatcg
      841 gtatatcgat gctcattatg aaatgtctga ttacgatgat atgcgtgcgg cgataagcac
      901 gatggagggc atttctggcg gcgagcctgt gttcctgtac agctcacaga atgtgaatat
      961 agagtatctc aagaatacca atgttgtttt caagccgttc ctgccagata ataactcgta
     1021 tatgagcaat gagaacttca aatatttcgg agataccgtt ataaggatag cggacagaaa
     1081 aaactggatg aaaagctttg aatccaatgt gacattgcct gttaaccctg cattatttgc
     1141 ggtcacgctt gacagctccg atgacaatga aaagatatca ttgttacaga cagtcggata
     1201 cacatatgaa gaagcccgca gatatattga tgataaaaaa gagtatgacc gctatgtata
     1261 tgacacttat tcctctgttc ccaagagcga aaaggataat ataaagaggt ttatagctga
     1321 gtttgaaagc aaagacggtg acaatgcagg tattgtcgat aacaaaatgc tctatgccta
     1381 cggaatgtgc gaatacctga gaacgcacta tcagtattct cttaacgcag ataatacatc
     1441 cgatcgcagt aacaccatgc tcggtaactt cttattcaat acaaaacagg gacactgcgc
     1501 attatatgca agtactatgg tgcttgctct gcgggaaaaa ggaatacctg cacgctatat
     1561 cacaggattt acaacaggta agcttaactt caacgaatat gccggtgtgt acgaaaagac
     1621 cattcatgaa aattcgctcc acgcatgggt tgaggtatac agcgatgatt tcggctggat
     1681 accgtttgat ccgaccgggt atggcggtaa cagtaatttc ggaaacagta cagatccatc
     1741 acagacggaa gtcccgatta cttctgttcc ggaaactacc acaactacaa cagtgcagac
     1801 gaccacaact gtatcttccg ataccgaaca gactactgct ccggtgacat cgtcacccga
     1861 cagcgacatt tccggaggta cggatggtgt aaacgaaagc aaaatcgatt tatcggttgt
     1921 gctcaggatc gcaatagcgg tgttggcggt aactgtggca tttgccgctg tcatcatatt
     1981 tataaattcg gtaaagcgca gaaacgaaaa gcgtatgaga tatttccgca agtcgaaaaa
     2041 tacgtcagaa gcggtaaaag aaatgtacgg ctttataatg aaactgtttg ccgctacgga
     2101 tatagccccg atcggcacag agcttcccga agaatttgcg ctcagagccg ataaagaatt
     2161 gctccctctc ggcatggata taagtcttgt cgaaataatg aagataatag aaaaggcgga
     2221 attttcaaat tccgatataa ctgaagagga acgcgcaaag gtgtacaagt acactaagac
     2281 gctttacgtt ctgattatgg caaactcggg taaattgaag cgtatgtggc tgaaagtcac
     2341 tatttgacaa taaatcggta ttattgttgt aaaatttctt tttatctgct ataataagga
     2401 tagaaacaaa aaaactgcaa ttgcaatgcg gaaaggaaat ataatatgag acttattacc
     2461 agatcagatt ttgacggact tgcctgcggc gcattactgc tggctgcaaa agtcgtggac
     2521 agttggatgt ttgctcatcc gaaagatatc caggacggac ttgttcccgt tactgaaaat
     2581 gatgtacttg cgaacgtacc gtatgcaccc ggttgcggac tttggttcga ccaccattca
     2641 agcgaagagg aaaggcttaa gataaaagga aagtataagg gcgaaagcag gatatcgcca
     2701 agtgccgcaa gaataattta tgagtattac ggcggtaaag agtgcttccc gaatttcgac
     2761 gatcttatgc tggctgtcga tnnnnnnnnn ngcagaagag atccttgaac cgacaggctg
     2821 gatacttatc ggatttctga tggatcccag aaccggtttg ggtcgtttca gaaacttcac
     2881 catcagcaat tatcagttaa tggaaaaatt gctggtatgt tgcgcaacta tgtccactga
     2941 ggagattctt gaactccctg atatcaaaga aagaataaag ctatacaatg agcaaacaga
     3001 gcttttcaaa aagatgttgg gagaacatac cagggtagag ggagatgtta tcataaccga
     3061 tctgcgcaat gtcgatccta tctataccgg caacagattt atgatataca gcctttatcc
     3121 gcatcagaat atttccgctt ggtttgtgnn nnnnnnnnct gtagctgttg gttactcaat
     3181 actcaatcgt acaagtaacg ttgatgtcgg ctcacttatg cttaagtacg gcggcggcgg
     3241 acacagagcg gtaggtactt gccagttcaa agacgaggag atcgaagaaa aactgccgtt
     3301 gctgcttgac gagcttataa attacgacaa atatcattct gcggcgcaca tataaaaagg
     3361 attgcgttga aacaacgaaa ggtacatata tgacagttca ggaatatgcg gtaaaactcc
     3421 gcggcaatat ttcaaaaatc attaagggca aggacgacag gatcgatctt atcataatgg
     3481 cgcttttaag cggcggtcac gttctgcttg atgatgttcc cggaacaggt aagaccgtgc
     3541 ttgcaaaagc acttgcaaag agtatcgatg caggcttttc aagaatacaa ttcactcctg
     3601 atcttttgcc gtcggatatc acgggcatac acttcttcaa tatgaaggag caggagttta
     3661 tgttccgtgc gggtcccata ttcacgaata tactgcttgc tgatgagata aaccgtgcaa
     3721 ctccgagaac gcagtcggct ttacttcaat gtatggaaga aaagcaggca acaatagacg
     3781 gcgagttttt tatgatagac aagcccttta tcgttcttgc aacacagaac cctgtagaaa
     3841 cctccggaac ctatccgctt cccgaggcac agcttgaccg ctttatcata aagctgtcta
     3901 taggctattc cgacaaaaaa tccgagatgg agatacttga taactcaaga gctgttcacc
     3961 ctgtagagtc actaacggct gtaaccgata agtcagagct tctcgaaatg atgagcagag
     4021 tggaaagtat aaaggtaaac gaggctgtaa gaggatatat agccgatatc ggcaacgcaa
     4081 gccgtcataa cagcgagata aagctcgggc tgagtaccag aggacttatt gcacttaaaa
     4141 atatgtcaca ggcaagagcg gcgcttcgcg gaagggaatt tgtgactccc gatgacgtaa
     4201 aggcggtcgc accgtatgta tgtgttcaca ggcttatttg cagaagtacg gttatgagag
     4261 atcccgacag cgcaaagcag gatattcttg aaaagatgat aaaggatatc cccgttccta
     4321 cggagcagat ttaaatggag cttgcagcta tcgtttttgt aatagcggca gttgttgtcg
     4381 ctgaggtgct tgttttcgga aagtatgcac ttaaaggaat acattacagt gcgtcggtaa
     4441 acaagaccga ggtctacgag ggagatattc ttgagctgac cgaggtcgtt gagaaccgac
     4501 gttttgtcgc tttaccgtgg atcaaaacgg agctttccgc ttcacgttgg ctttcgttca
     4561 atcaaaagga ctcagccgct cagacaagcg gcggcgataa catattcata ccgggagttt
     4621 tctcgttaaa gccacgcagt aagtgtatcc gcgtaagaag gataaaatgt cgtaaaagag
     4681 gcgtattttc ttttgatgat acggtagtta ccgcaaccga tatgctcggg cttgtaagag
     4741 tgtcaaacag cataaaggtc ggaataagta taacggtgct ccctacagcg tcagacggag
     4801 aagatgctat gttgtctttt aacgaaccga taggcgagat acttgtaaga cggtttataa
     4861 atgaagatcc gtttatgata gcgggcgcaa aggagtatac aggcagagag ccgctcaacc
     4921 gcataaactg gaagtatacc gcaattaccg gaaaacttat ggctatgaat aacgaattta
     4981 caacatcgcg aaattcgctt attgttataa atatgcagag aaaaggcgtt gtgcctgttg
     5041 cttctgctga tatcagggat accgaggcgt ttatcaagct tgccgtaaag cttatgtggg
     5101 acagcgttga cagcggttgt agctgtgcat tcgcaacaaa cggcggaaag gcgggaggta
     5161 ctgcgtccgg ttttataaga acaagagagc agtgcgaggg cacactcaga atgctggcac
     5221 agctccctga cagctgtacc tatgaatttt caaagttcct ggatgcgctt aattacagca
     5281 gctttacaga tgtatttata ctgacaccgt atatagatga tgatatgact gcattcgcag
     5341 ataggatcag tgcttccgat ataagcacgg cgttctatac aacaggaaat atccctgaag
     5401 atactgacgg tcagtatctt cacgcaaaga aattttcgta tacattcaat acggatcgcg
     5461 aggaggacgc aaagtgaaaa agaactttcc gatgaaatgc gttgccgcac tggcaatgct
     5521 tcttctgcct cttcctatgc tgtttgtatt tgaagtcagt gcatttggcg gatttacctt
     5581 gtggcactat ttgctttatt ataccggtgc aggaattacg gtgggaactg cgtattttct
     5641 tatgtcgcta aagctaagcc ttcacagacc gaaagccata ttcgctgtaa atgcgatgac
     5701 tgtattcatc ggaattatct ttacggcggc nnnnnnnnnn gcagttcctg tagcatcaac
     5761 ggcggtcaat aacacacttg acgaaaacgg ctttattgtt ttttcgctgt gccttggact
     5821 tatgccgtcc gttatagttt ggtatctgct cggaatgagt cttagaaaaa acgcctttga
     5881 cgaagtattt acacctgtat ggctcgggat atacattgtc gaaacattct tttgttatat
     5941 tttctgcggt gctatggcag aggataaagc gtatctcaac gactcgaaga caaagatagc
     6001 ggtacttctt gtagtgatgg ctcttcttac tgtactgctt ataaatcagt cgaatataca
     6061 gtcacagatc gacaggagaa gaaacaccaa ccttatagta cctaagggac tcaaactgta
     6121 taatgcgaag cttataggta tagtcggcgg cataatcctt gcggcactgc ttctcaagga
     6181 ctatgttgcg gcggggctta catggcttgt gc
//
LOCUS       DAWAOG010000047         6202 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1720_length_6202_cov_1.963559, whole genome shotgun sequence.
ACCESSION   DAWAOG010000047 DAWAOG010000000
VERSION     DAWAOG010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1720_length_6202_cov_1.963559"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1328..1337
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4728..4737
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5697..5706
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1531 a         1452 c         1386 g         1803 t
ORIGIN      
        1 ttttgcaagt acaattactt gagcagggat tctgtagccg ctatctttac tgcaacgcag
       61 aatacctcca tagcctttgc aagctctgct tctgtaggat atgtcggagc aatacggata
      121 ttgttgtcgt gagggtcttt gccgtaagga taagtagcgc ctgcgcctgt cagaacaact
      181 cccgcctgct tgcaaagctc aacagtacgc tttgcagtgc cgtcaagacc gttgaatgag
      241 atgaaataac cgccgttggg cttgtgccat gagccgaagc ctgtgggagc aatctccttg
      301 tcgagcatat caagaacaat cttgaactta ggctcgatta gagctctgtg cttcttcata
      361 tgctccttga taccgtcaaa atccttgaag aaacgagcgt gtcttaactg gttgagctta
      421 tcgtagccga tagtctggaa tgtcatctgg ctcttgataa acttgatgtt ttccgcactg
      481 gcaccgatga atgcaagacc gccgccgggg aacgagatct tcgatgtgga cgcaaacata
      541 aataccatat cctgcttgcc tgtcttttta gcttcttcaa ggaggttcag cagagtgtcg
      601 ggagtatctg tgaggtgatg aacgcagtaa gcgttatccc agaatattct gaaatccttt
      661 gctttaggcg aaagatttgc aaaacgcttt accgtttcgt ccgagcaggt gataccttcg
      721 ggattgctgt acataggtac gcaccagata cccttgataa gctcgtcttc gcttaccagc
      781 ttttctacaa cgtccatatc gggaccatcg ggggtataag gaactgtaat catctcaatg
      841 ccgaggctct ggcagatagc aaagtgcctg tcgtaaccgg gggcggggca gaggaacttt
      901 accttctcgt tctttcccca gggcttgtcg ccgccgagta cgccgagcag catcgctctt
      961 gccattgtat cgtacataag ctgaaggctt gagttaccgc cgatgattat ctcgtcatga
     1021 gcaacaccga gcataggcat acaaagtctt tttgcttctt ctataccgtc aagtacaccg
     1081 tagttacggc agtcaacacc ggtttctgac atataatcac cgtcaaggca atgcgtcagc
     1141 atatcaagcg ataaatcaag ctgtgcagga gagggcttgc ctcttgacat atccagcttc
     1201 agaccctgct tgcaaagctc gtcatactgc tttttagctt cttcattcag ctttttcagt
     1261 tcattctttt ccattattgc aaactcctta tgataaagaa ttttaacgca gttcttcact
     1321 gcacccannn nnnnnnnatt tgcaagcgtt ttttcgtttt cttgcaaaga attatgcaat
     1381 tttgtcaaaa aaacgttgca cagtaacata attatggcga ttagcaacta taaaatatcc
     1441 attaaatgtt taatctgtgc aactcgaaat tttgattttt gcatcaagcg gcaaatgcgg
     1501 ctgtcacaat gccgttgttg tttgtgaatg taatgtcggt gctcaccgtc tgctcgtcaa
     1561 gccccgatag gttcagcgga acgctgcagc gtacattata cgtccattca aaagtcagcg
     1621 tcgtacttgt cttgccgtca agaaaagcaa ggctcatatc aaaatcggtg ctgtcgttca
     1681 gaagagccgt tgcggataat gcatcatcat atattaccgg gagggtaacg ttccggcggg
     1741 taagctctgt gggcatgatg ttgcgcattg aaatgagctt agaatttccg tccgtgtaaa
     1801 cattaagcgt aaggtcggca cagtagaagc ctacctgctt ttgaccgtca agcggtacat
     1861 cttctgcata tccctgtacg gaaaaataac ctgttccgta gtcggataat gcaatgctga
     1921 atgtagggtt gaataccgga gtgccctttg ccagtatagc gtcatatgcc gtcagtgcac
     1981 cctcgactcc gtcctgaaca tcaagcagag cggtttcgac ctctgtcttg tcaagtcccg
     2041 aataatagct tgcggatacg gcaaagcttg agccgtcgct tataatttca ctgttactgc
     2101 cggcatatac gggagttgtg cgtgtaagca gcatatacag taaaagtcct attcccgcag
     2161 taacaagtac aagcaggata acaagtacga tttttttcat ttatgctccc tttcagatgc
     2221 agtaaagatt catatcccat gcggggatat acggatgctg tcttaaatac agcttgtacg
     2281 cggggttaag ctctttgacc ttaagcggaa gagcaaacat atcctcggtt cgatgataaa
     2341 gagaaacaag gagtttaggc ttgtcccgct gtattactgt tttactgccg tcaagcgcct
     2401 cgctttcact gccctcaacg tcatatttta tataagttat gtgctcgtcc tttacaaagc
     2461 tgtcaagact gcgcatatct atttctttga ttcttgacgg atttatcggc ttgccctttt
     2521 cgtaagggat aagcgatgaa tttcttccgc cctttgatat gaactcgcat actgtgtcac
     2581 agctccatgc gcctgcattt accgtcctga aatcataagg gttcagcttg tagtgtatgc
     2641 gtttcatctt tgcgtaattc ttcccgtcag gctccattgc aaaaatcctg ttgaagcgcc
     2701 cgccggtgac tctcagaaac tcggatatgg tatcgccgtt gtacgcacca aggtctgcat
     2761 aggtttcgtt ttcaccgagc gcaaggatgc catagccgtc cgatttgtct gtctgattgc
     2821 ggataagcgg ttgtattctg cctgttatac ggtattcgag ccagccgtca aaaacctgct
     2881 ttgatttttc gtccgaaagc atcgagtaaa ctctttcgag ctcggttctg tgttcttcgg
     2941 catattcttt tgtgaataat ccgccgccta tcacgggaac atcaggggca tataattcgt
     3001 gtctgtccga aatggctatg atccgctgta tcacctcgtc aatatgcgtt ccgaacgcaa
     3061 cgagtatgat acagtcacca aactgctgtt caatatctga cagttttttt acctcaaagc
     3121 ccgcaaagct gtgacctctc acaaagtcat cgcttgccat aaagcccgat actttcacat
     3181 tcttttcggc gcagacatca agtattttct gtgcgccgtc acccatgccg tatattatta
     3241 tcggcttcga ggtcgcggct aagtgctccc aaaggcaaac cgcatttgtt atatcgtaca
     3301 tctgaacatt ccttttccgt gattattcga cagttaccga ttttgcaagg tttctggggt
     3361 ggtcaacgtc aaagttttta agtgccgcag tatagtatgc gataagctgc aaaggaacta
     3421 ctgccacaag cggcatcaga agatctctgg caagcggaag cataacggga taatcaacat
     3481 cgttttcgtc aaacctcata tcttcgctgt agaccattat tacagttgca cctcttgttt
     3541 ttacttcctt tatgttgctc atagtctttt cataaagagt gtgctgagtt gcgactgcaa
     3601 tcacaggcat tccatcttca ataagcgata tagtgccgtg cttcagctcg ccggcggcat
     3661 agctttcgga gtggatatat gagatctcct tgagcttaag gctgccctcc atgctgagtg
     3721 catagtcaag acctctgcct atatacagca ggctgtctgc actcataagc tttgaggcta
     3781 ccttctgata ttgcgtctgg tcgtcaacgc atttctgcac ttttgacgga atttcggcaa
     3841 gctccgatat cagctgtctg cactctgctt cgtctatggc atttcttgcg tatgctacgc
     3901 ggaaagccag cagatacata aatgctgtct gcaccatata tgcctttgtg gagcataccg
     3961 atatctcagg accggcaagc gtgtacatta ccatttttgc ttctcttgct atagatgaac
     4021 ctactacatt gacaatggca agcgtatccg ctccgataga atttgcaagc ttcaaagcgg
     4081 caagcgtatc tgcggtttcg cccgactgcg agattattac cacaagatca tcaggggcaa
     4141 gcagaggatc acgatagcgg aactccgatg cgatatccac aacaacagga gtgcgcgcga
     4201 gcttttctat gacgtatttt ccgaccatac cggcgtgcat agcagaaccg catcctacta
     4261 tatagatatg atggtagctt ctcagttttt catcggtaag accgcattcc gagaagtcgg
     4321 gcataccgtt tttgatccgg ggatttatag tttttttgag cgcatcgggc tgttcgcata
     4381 tttccttcag cataaaatgt gcgtaaccgc ccttttcggc tgccgaaacg tcccagtcgg
     4441 caatctgtat ctccttttct atgacatttt tgtgcatatc ataaaatgtt acaccgtctg
     4501 cagtgatatc cgcaacttct ccgggttcaa gcagatagta ttctcttgtg tattcaatta
     4561 tcgctgtgac atcggacgcg ataaacattt cgccctttcc cttgccgact atcagaggac
     4621 tttcttttct tgccgcgaat atgcggtttt tgtgttctct gaacattata ccgagcgaat
     4681 aagcgccctc tatatcagca attgtcctga ttatagtatc aatagggnnn nnnnnnncaa
     4741 tagggtcgcc gtcgtagttg tagtcaagca gctttgcgac agcttccgta tcggtctgac
     4801 tttcaaagcc gtagcccttt gatatgagaa attctttcag cttgcggtag ttctcgataa
     4861 taccgttatg aacaatggat actctgccgt ttcctatagg gtgagagttg atatcgctcg
     4921 gctcgccgtg cgttgcccag cgtgtatgac ctatgcctgc ggtagcagtg aagtgaggtt
     4981 cgttctcgag ctttttgata agaccgtcct tgagttttcc ttttgctttg acggttttta
     5041 tccaaatacg gctatgcctg cggagtcata gcccctgtat tcaagcttcg tgagcgaatt
     5101 tacaagtatt gcggtgcatt cttttgcacc gacatatcct actattccac acataaattt
     5161 attccgcctt tcgtaaaccg gagtctgtat atccgagata tacgctataa aatactattc
     5221 tgatttttct ttaataagta caaagccgtc ggcttataca aaatacagta taacacattt
     5281 gacgctaaaa ggcaagtgtg ccgatgcaat tgatgttgac acggtcaacg gttcgtgata
     5341 aaatatagta acacaaacga ttcgatttgt gagaaataat attatgaaaa aagcaagaaa
     5401 taatgttctt gaaggaggaa ttacgatggt ttcacaggca gagtacattt acgcatatct
     5461 gtcggaaaat atgttcgttc cgactgttat gttctgggtc gctatagcca tagccattac
     5521 atctgtagta tgttttatcc ttgcgccgct ttacagtaaa ggaaaacacg gtggtgtgaa
     5581 agtcttggcg cagatcatta caatactcga ctttatcggc ggtatcggtt gcatactggt
     5641 tttcgccggg gtatcagtgc tttcgcttct gtcagcagac ggtgcattcg ctgacgnnnn
     5701 nnnnnntaca ggaaaaaata ctcgggatac tgcttgctcc gacaaggttc ggtatgtcgc
     5761 ttgacagctt tgtatccgga gatctgtcgg cgggcactgt aatactcttc tacggacttt
     5821 atttcggcgt ttccggtttg cttggcataa tcacgtttat tgtcaacagt tcggcaagag
     5881 gtaagtataa cggcataaac ggaaagataa gaggtgcagc tgctcctcag ggagcgcccg
     5941 ggtataatcc ttatcagcca tatggtcaga acggacagaa tattccgcag ggctatcccc
     6001 agcagtatca gcagaatgta ccgcagggct atcctcagca gtatcagcag aatgtaccgc
     6061 agggctatcc tcagcagtat cagcagaatg tacagcaggg ctaccctcag cagtatcagc
     6121 agaatgtacc gcagggctac cctcagcagt atcagcataa tgtatcacag agttaccctc
     6181 agcagtatca gcataatgta tc
//
LOCUS       DAWAOG010000048         6197 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1722_length_6197_cov_2.451970, whole genome shotgun sequence.
ACCESSION   DAWAOG010000048 DAWAOG010000000
VERSION     DAWAOG010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1722_length_6197_cov_2.451970"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1526..1535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2132..2141
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5440..5449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1772 a         1345 c         1374 g         1676 t
ORIGIN      
        1 gcacagctgg gcaaaataac ttataactcg gtagataaca cgacgactat agatacagca
       61 gaaatcaatg cggcagatga ttttgacagt cttttctgca cggataagca gattcagagc
      121 gaagtgctta atctgttcga taatgcaatt gacatcacaa acaaatatat catccttccc
      181 gacagcaatg cggcggtagg catatcatcg gatatgagtg cgcaggaaat agaaagcttc
      241 aaaaacaaca gtgccaactg ctttgtcggc acgggttatt atgaaaacga acgtttttat
      301 tatgctgaca aagcgtattt cggttttgac acctcagagg aatttataaa ctccgtatca
      361 caggtatata cgggaaatta ctcagataat tttgcggaag atttttataa ttactgcccc
      421 gattacattc agccggcgga taaaaataac ctaaaatcga atttcggtat aaatatcggc
      481 aaagagcttc cgataacaga accctactcg gcgcacacac agtattgtgc ggctgtttat
      541 acagacgaaa gccataattc tttgatactg ttatcgctta ccgcaaaaga cggtgctaac
      601 gacagcaaaa cgtttgaact cagatatgta cccatgaaat atgttgacgg aaagcttcgt
      661 atagttgttg agatatataa cacgattttc atggacgtaa acgcattctc cgataatttt
      721 atgtacaatt gcggaatggc aagcaaaata gaggcagacg cgaaaaccgt tgatataacc
      781 tcagtaatga gcaacagtga cagctcatca aagcccgaag aaacaacagt gacgacgaca
      841 gttaccacaa cgacttcaac cacgaccgcc gcaacatcaa caactcccga aaccgaaagc
      901 acgaccgcac cgcagacaac cgccgcgccc gaaaccacaa ctgccgcacc cgaaaccaca
      961 actgccaagc ctgctcccga gccgacaggt actgcgacta acgtgacgct gagaatgaca
     1021 ggcgatgact cttatcttca gaaaggatat ctgtattatc tcaaggtcga cagcgaattt
     1081 ataaataact ccgatgttct tgaatacacc tcggacaaga tgagtgaata tcctatgttc
     1141 agcactgacg gaagcgatca cacgtttgac agcatagaat gcggcacata tattattgca
     1201 ggtccgtgga tctgtacgac aagcgacagc ggatatgttg cattcagcgt aacggaaagc
     1261 gacagcaacg gtgtaataaa tatcgatctt aacaacggaa cggttgccgc agataaaata
     1321 acggtcgtaa gcccaagcac aatgcccgat atcagcgagg aacagatcgc acttgcaaac
     1381 gagcctattg attacgaagg acatacttac atacctgttg agcttgtatc atataatgta
     1441 gggtgccgtt tagaccttga cgcgcaaacc gtgaacaatt acataaagac tgtaagcaaa
     1501 catgagatat tcggaagcaa agcaannnnn nnnnntggtt gacgggaaga tgataacggg
     1561 tgatgaaata aatgattatc ttctgtttgt aagtgtattc aaagttgaag gtgagagcca
     1621 ttttaatctt aacaccgtta tggtaaggca ggatcttgta agcaccgaaa agccaaagct
     1681 tattctgagc gacgactact accatatatc ttttcaatcc gactatctcc ccggtaacga
     1741 agacatagat atgatactca aagatgacaa cggaaagcct gtgaagaact gcgaaatata
     1801 ttttgtacat cttgatgatg tcggagcata taatcttgaa cacggcatcg cagatctcga
     1861 tcaccacggc gactactgca tagcaaacac ggattcggag ggaagagtca ctgcgtctac
     1921 cgcgctgtac tgcggaaaaa cctatctttc gataccggct gacgttatcg aaaaaggcta
     1981 tgtattcaat aacgatcgtt attatgacat tatcccaaaa gatcctgtgc caaagctgat
     2041 agcggaaata ccgattacaa acgaataagc gattattacg gcgacagcgc cccatcacaa
     2101 gacggcgcgc tgtcgcttta tatttataag gnnnnnnnnn natacagatg ataacacctg
     2161 cgaagatgat ctcccactgt gagccgtaag ggccgatgaa cttgtacagt gcagtggata
     2221 cgacctgaag tccgtcactg ggcatataca ggtttgctgt atagaagtcg ttgtatatgc
     2281 ttacaaactt tattacaagc acagttgcta ttgcgggtcg gagcatgggt aggataatgc
     2341 tccagtaaac acgcggatat gaacagccgt caagtatcgc gctctcgtca agggataccg
     2401 agatattgtt gaggaactgt atgaagatat agatagaaac tatatccgta ccgatataaa
     2461 gaactatcgg cgcggcaagc gtgtcgtaaa gtccgagtgc gtgcactacc tggaatactg
     2521 tgacctgcat tgagatgttg ggcagtaaag ccgcgataag gaatatattc ttgacaagct
     2581 ttgtgaacac cattgtaaaa cgctgtacga caaaggctgt cattgttcct gtgataattg
     2641 tgccggcgca cgagaacacg agtatgatag ctgtattgat aaagcccttt aatacattac
     2701 cctgaatgaa tgctgtcttg aagttttcaa gttccactac tttaggaagc tcgaaagctc
     2761 cgcttttaag gaagtcctgg tggcttttca gcgaacccag cagcacaact accagcggca
     2821 caagaaccat aaggcacgct acaaccagaa caacatattt cagtacgttt ataaaaactc
     2881 tgacaccgtg cggtgtttct ttgaaacggt acttttccat attgaacgag gtctgctctg
     2941 cgacttcgtt tgtcttgata ttgtctgtca tgagttcacc tacttttctt ctttgaagaa
     3001 catcttctgg atcaccgtga taactattat gataaacaga agcacgatag ccattgcgga
     3061 cgcaagtccg atcttctgat actggaagcc ggtttcgata gtcttgataa cgaatgttgt
     3121 agttcccatc ttgccgcccg tgacaatata cgggatctca aatacggaag ccgcaccctt
     3181 gatagcaagg ataagctgta atgcgatgat cgtcctgata ccggggaaaa ttatgtgcca
     3241 gaacttctgc catgcgttag caccgtcaag gtctgccgcc tcgtaaatgt caggcgagat
     3301 tgactggata gcgccgatga acatcaggat atcaaagcct atataacgcc atacagacgc
     3361 gaaaaccaga caccagttgt tgatagcgcc gtttgacagc cactttacgg gatctgcgcc
     3421 gaaaagggcg attatggagt tgagcgtacc gccgtcatca cctctgaaga agcgctggaa
     3481 aataagtgct accgcaacac cgttcataag atacgggaag aagataactc ccttgaacag
     3541 tcctgcgagc tttatcttcg agcaaagcag tgttgctatg aaaagcgcaa ggccaagctg
     3601 tatgaatgaa cctacaaaat agtacaagct gttgatgagc gttgagagat atgacatatc
     3661 ggtaaacagc gtcttataat tgtcaaaacc tacccactgc acattaacac caagctggtc
     3721 acgctgctgg aagctgaata tcaccatatt cacagcaggg ataattgtga aaagtatcag
     3781 cagagcgaca ggtatcgcca ggaacagcgc tgtagtcata tatttctgac cggtgtagcc
     3841 tttcagccac tccactaaag tacgccgttt ccgaactgcg gtaacgcctt gtgtagccat
     3901 aattaccgtc ctttcaagaa ataatttcta attttagcaa aaattaaatt actcccatat
     3961 aaacctgtac ggaaagactt tacgcctttc cgtacaggtt tgtgtgtttg gagtatctat
     4021 gaagaaaata cgatagcgtt gattagccgt tattcttgat ataagcagcg agatctttat
     4081 ttgcatctct tgtagctgcc catttctgat taacggagtc tgcgatagcc ttaaagccgt
     4141 catcgccctt gcctgcgaat gctgcttctg caagctggag cttgaagtta gccgagtcgc
     4201 cgtcccatat acctacttct gaatccttgt cgatagcgtt gaatacgcct gtcagaccat
     4261 cgggagctgc atttgctgtg aagaattcgc atccgtcaaa tgctgagagg taatcgggga
     4321 gcttactgcc ttcaagtgta ttgataccgc cttcagcctc tgagaagcct gattcagcga
     4381 tgaaccattc gatatacttc ttaccgagat ccttcttgtc atcggaagaa ttcttgttta
     4441 tgcccatgca gtagtcagcc gcatactgtg catactgctt gccgtcctta gagaagggag
     4501 cgggcatata accgatgttt tcgggatcaa gaccgttgtc cttagcgatc tgctggaact
     4561 gtgaaactgc ccatgaaccc attgtcattg tagcaacttc gccgttagcg agcataagct
     4621 ttgagccttc ccagtcagaa ctcatcgggt cgccctcgat aagagaagaa tctgaatata
     4681 tatcatacat cagcttgaat acgttgtagt aaggttgacc ctccttgaag aagtcttcct
     4741 tgtttacgat gaggtttgtt tcaaagtcag gatcgcctga tgaaggaaca acaaggcttg
     4801 accactgtgc agccgtccag cttgcttcct tgaagtttgt ataataagga ataacctcgg
     4861 ggaacttttc cttgatcttc tttaagtcag cgataaattc atcggctgtc ttgggcattt
     4921 ctgtaatgcc tgcttctgtc cataccttct tgttgtagca gataccgccg cttactgtgc
     4981 ctaagtgtgc aagtccgtat actgtaccct cgtacatctt ctggttagcc cacttgtact
     5041 tatccttgag ctcgtcgtac ttaccgagag gttcaaagaa gtttgaaaga tctgcgagct
     5101 ttactgtatc ggggatcatc agaacatcgc cgtactcctt agtgctcatc attgttgaaa
     5161 cgtcaccggc ataatccttg aatgactgat attcaaccgt gcagttgtac ttttcttcaa
     5221 aagcgtctgt catcttgtcg agtgagccgt cgtccttacg gtctgtacgg tgtgtcagaa
     5281 ccttaagtgt tatcttttcg cccgagggag ttgtcgaaga agcttcgtct gctgttgttg
     5341 catcgctctt gctttcttct tcctttgacg atgtagatga tgttgtgcct gttgatgatg
     5401 ttgaattgtt gttgctgcag gctgccagcg atactactgn nnnnnnnnnc tactgtgctt
     5461 gccgcaagaa tacctgcgag tatcttcttt gctttcatgt gataatttcc tttctggtcg
     5521 gacagtgctt aatcacttat ccttgatcgg taatttagct tatatttatt aagctgattt
     5581 agttcgctta attcacttac gattatacac cgttttagct aaaagtcaat aagcgtatag
     5641 gaaattcagc taaaacgcat aaaatgtatt tgtgctttat gtgcatacaa tacaaatatg
     5701 ctattaactt agctttgtta cacagtatag ttaagcaagt gtctgtggaa aacgttttca
     5761 ccgttacaaa ggtgtttagc taatttcaga ttaaattaag ttacattcaa ttcacattgt
     5821 tttaggccgg ttgttaatag gataactcga aataaaacag gtatacattt aatgtggata
     5881 attcaaaaga aagttgcggg tgtgggggaa gtgtggtata ataatgatat atggtttttt
     5941 tgtttttgat ttcggcttgg cggaaatgtt tgatgctttt actatggtag caggctaccg
     6001 atagttctgt ttgtggggta gcttgctacc gatagttctg tttgtcggat agcacttctc
     6061 gggttcgcta ttctatgcga taacgttgct gctatgaacg agggagacaa ccaaagggac
     6121 tagggtaagt ctgagccttg catacgcaag gctcgtgcac tccgctaaag ctccgttgga
     6181 cttggcagcc cagtgaa
//
LOCUS       DAWAOG010000049         6166 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1731_length_6166_cov_2.193094, whole genome shotgun sequence.
ACCESSION   DAWAOG010000049 DAWAOG010000000
VERSION     DAWAOG010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1731_length_6166_cov_2.193094"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1101..1110
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2695..2704
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1545 a         1616 c         1222 g         1763 t
ORIGIN      
        1 ttacagcact catcaacagt atcggtgagc ttgccctcat acagcttgtc aagacctgca
       61 atagtcgttc ctcccggaga agaaacctgc ttaataagct cgtccaccgt catgcccgag
      121 tctgtcagca tcttcgcaga gccgacaagc gactgtgcaa acagcttgag tgccgcatcg
      181 gcatcaatac ctacgctctt ggcgtaatct acaaagcctt ttgcaaacag atatataaaa
      241 gcgggagaac tgccgtttac ggcgattatt tctttcatct tatttatcgg tacttcctcg
      301 gcaataccac agctttcgat aactgacttt gcaaatgcaa attcttcatc ggttactccg
      361 tcggctcttg atattgcgct tgcaccgagc ccgagcatca tcggagtatt aggcataacg
      421 agcactacct tggcattcgg tattgtatga ctgcggataa agtcctcgga tattcccgca
      481 cagatagaaa taatgacctt attatgcgtt acttcatcgg cgatagtatc aagcacaccg
      541 ccaagcacct gcggctttac cgcaagcagt atgtaatcgc actttttgac aagttcacat
      601 tcacttgcac aaggcttaac cccaaatgcc gaaagtgctt caagctttgc cgtatcctta
      661 tcatacgccg aaacgcttac ctgcatatca gatgagcaga taccgcgcat tatcgcactg
      721 cccatattgc ctgcgcctaa aaatccaagt tcagtcatac tatatcctct tttcttgttt
      781 caagccgaac gcttttctct gaatgccgca cgatatgcca ccataacagg ataaagaacc
      841 agcagaagta tgaatacctc tataggcagc atcgtcagat ttttaactat tcgtgtcgct
      901 atcttgaccc agaatacttc aggcgcaaag tatcccatga taaccattgc aacggtattt
      961 aaaaatacat tgcagaaaag gtttacacag aactttgcca taaatattct gaacacctga
     1021 aaaccatttg tctttagccc gccaaaaaat gcggttctgc ttgaaaaatc aagcttaacg
     1081 ggatttagtc cgtacaggaa nnnnnnnnnn cggtaggctt tacaagaaag ccgattacgt
     1141 cggtgacaac gcctgcaagt cctgccacaa caggaccgta cagcatacct atagccgcaa
     1201 gaccgacaaa cgcaaaactg attttcagcg tttcactcgg cataatcata acgaatttca
     1261 gtgctaccga aacaccgata agaagtgccg ttacggtaag gcaacgaata gatttcagtt
     1321 cctcgaacga ctttttaaaa ttacgaaaaa acgccatatt tacctcctca atgacgcaaa
     1381 cttacgcgat cgcataatgt gggataaata cggcgattat ctcttatatg caacagcgag
     1441 atgcggaaaa cgtaccgcca cctttcagtg ttcgtcctga ggacaactcc ccgtccctca
     1501 ggcactttac gcacgttaac ctactctgtc ggctgtaccg ttaaaattcc ggcacagtct
     1561 ttatataacg gaaacccgtt atagtccttt taaaatcaga agcttatctc attagcgatc
     1621 ttgtacagat cgtgagggaa ttccttcttc atcttgagag cttcctgaat gtcatagttg
     1681 acaacttcat tatgctgcat acctactaca cgattaccga tgccctttga aagaagatct
     1741 accgcataat aaccaagctg gcttgccgct actctgtcct gaacagtggg gctgccgcct
     1801 ctctgaacgt gaccgagaat tgtagcacgg ctctcaacac ctgttgcctg ctcgatttcc
     1861 tttgcgagct gttcgcttcc gccgatacct tctgcaacaa taatgatgaa gtgatgcttg
     1921 ccgctcttct gacctttgat tatctttgag ataacatcgt ttactgtata gggctcttcc
     1981 ttagtgatga tgtatgtcgc accgcaagcg atacctgcat tgagtgcgat atagcctgca
     2041 tttctgccca tgacctcaac tacagagcat ctgtcgtgtg actgtgctgt atcacgcagc
     2101 ttgtcaacca tttcaacaac agtgttcata gctgtgtcat aaccgatcgt gtactcggtc
     2161 attgaaatat cattatcgat agtaccggga acgcctatgc tcaggatgcc tcttgcagaa
     2221 aggtcagccg cacctctgaa cgagccgtcg ccgcctacaa caacaagacc ctcaatacca
     2281 ttctcttcac aggtcttctt agccttctga agaccctctt catatctgaa ttccgcacat
     2341 cttgccgtgt aaagcattgt gccgcctctc tgaattatat cagatacact tcttgcatca
     2401 agagggataa tatcaccgtt gataagtccg ttgtatcctc tgcgaacacc tacgacttcc
     2461 attccctttg caatagcagc tcttgttacc gcacggataa cggcattcat gcccggtgca
     2521 tcgccgccgc ttgttaaaac gccaatcttt ttcataatat cgtacatcct tccttagagg
     2581 gcgccctcct agattattta cacacatctt agtttactcc taaaatgcaa tttagtcaag
     2641 gggtaaattc atttttttga cttattttat aattatattg tttttgccgc agatnnnnnn
     2701 nnnntcaaag catatcttag taagagtgtt acccttattt gctctgagca atgcctttac
     2761 tgcgggaata ttcgtatcat tccgtgacac aaagcgtata tacagtgtct ttttcccgct
     2821 ctgctttgcg ccctgtccgt tcatcgtttt acgaagcttg tcaagatcag gctcggttat
     2881 catatttatc agcagcttcg gatcgccctc gtcctcaacc gaaacagtcc cgtcaaaggc
     2941 atatactttt cccgtttcaa gcataaccga atactgttca tatagcttcg gaaacacaag
     3001 acattccatt tcgccgctca tatcctctat cgccgcaaag cacatagttt tatttgattt
     3061 tgtgatatgg gtgcgctttg aggtaagcaa tgccactatc tttacatttt gcttatcctt
     3121 cggatgctca agtatatttc cgatattagt gtatccgcag tttcttgcta ttttgtagta
     3181 tggctcggca ggatgacccg aaatatatat tccgagcatt tccttttcga gcgacaggag
     3241 ctgtgccgct gaatactcgt cggtcggctg tatcggatac cccgtcagat ttgctccatc
     3301 gctgtcgccg aacagatcca tctgaccttc aatatcacgc tgttgtgaaa gtgcgtcaag
     3361 catcagttca aatgacatca tcatttcacg gcggttgttg ccaagtccgt caagcgcacc
     3421 gcattttata agcgattcaa gcgcgcgctt attcatattc ttatccgaca ttctgtcgca
     3481 gaagtcaaac agcgatttaa acgggcgttt atttctctcc tctatgatat cggctatcat
     3541 atttgcgccg acattcctta ttgcaagcat accgaagcgt ataccgtcct tttcgcatga
     3601 aaagcctgtt tcactgtagt ttacatgagg tggcaggagc ttaagtccgt gctgtgcaag
     3661 gtcgcctata tattctgcgg tctttccgcc ccagcctatc acgcttgtaa gcagtgccac
     3721 catatattcc ttgtaataat gacacctgag ataagcggtg cgataagcaa gcagcgaata
     3781 tgccgccgca tgggatttgt tgaacgcata gctggcgaag ctcatcatat cgtcaaatat
     3841 ctcttctgcg acctgcctgt caacgttatt ttttacagca ccctcaacga atgtcgcacg
     3901 ctccttttcc ataacctcgg gctttttctt agccatagct ctgcggacta tatccgctct
     3961 gccgaatgaa tatcccgcaa gcacacggca tatcatcatt acctgctcct gatacacgat
     4021 acagccgtaa gtaacgtcaa gtatatcctt taaaagcggg tgcttgtatc gtatcctttc
     4081 ggggtggcgg cgattttcca gatatgtcgg tatagagtcc ataggaccgg gacgatacag
     4141 cgacagcgcc gcagtaagat cctctatcga gcatggcttc atacgcgata aaagcgaggt
     4201 catacccgca ctttcaaact ggaatattcc gagcgtacct cccgttgaca tcatatcata
     4261 ggtcgcactg tcgttttcgg gtatcttttc tatatcaaaa tcgggatcgt ttttctgcac
     4321 aagtttacag cagtcctgta ttacggtcag ataacgcagt ccgagaaagt ccattttcag
     4381 cagtccgagc cgctctatgg tagtcatcgt gtactgcgtc ataacttcgc cctcgccgtt
     4441 tctgtacagt ggtacataat cggctacggg gtcacgggtt ataactatac ctgcggcgtg
     4501 tattgaggta tttctcgcca ttccctcgat cttcctggcg gtttctataa gcagacttac
     4561 ctcattatcc gatgaagcca gcttcacaag ctccttttcg cttttcagcg caccgtcaat
     4621 ggttgagtgc atcgacatag gaataagctt tgcaaccgta tcgaccttgc cgtaaggcag
     4681 ccccattgcc ctgcctgcat cacggatagc acccctcgcc gccattgtac cgaacgtgat
     4741 tatctgcgcc acatgatccc tgccatactt tctgacaacg taatcaatta cctcctgccg
     4801 tcttatatag caaaagtcta catcaaaatc aggcatggtc acacgttcgg gatttaagaa
     4861 acgctcgaac agcagattaa atcgtatcgg gtcgatatct gtaatcccaa ggcagtacgc
     4921 gcagacgctt cctgcgcccg agcctctgcc cggacctact gcaatatcgt ttctttttgc
     4981 ataacctata aagtcggcta ctataaggaa gtagtcgata aaacccattt tctttataac
     5041 cgacatttca tattccgcac ggtcggacac ttcttccggc acagggtcgc cgtagcgttt
     5101 tttaagtcct tttcttacct gcccggtaaa atatatctcg ttgttctcat acccctccgc
     5161 cttgaaatac ggcagtattg tatgaccgaa cgtgaaggcc acattgcact tttccgctat
     5221 cttgacggta ttgtcatatg cagactgcgg aaagcctagc gctttcatct cgtcctcgct
     5281 tttgaggtaa aactcgtctg tcggaaactg catatcactc ttgtcgttaa gcgttgtatt
     5341 ggtcgctatg caggtaagca cacgctgtat atagctgtcg tccttttcga catagtgcgc
     5401 atcattggtg gcgacaagct gtgtaccggt atcccttgcc acacgcagaa gatacggcag
     5461 tatgcgttcc tgctcgctta taccgtggtt ttgaagctct atataataat cctcaccgaa
     5521 tatatcctta tattctttga caaggcttac agcgtccgca agtctgccgt caagcaacac
     5581 cctcggaagc tctcccgaaa ggcaggcaga aaggcagata agtccgtccg aatatcgtct
     5641 taaaagctcc ttatccactc tcggtttatt gtaaaagcct tcggtatatc cgatagtaac
     5701 aagctttgaa agatttttat atccctcgtt atctttacac agcagtataa gatggtgcgg
     5761 cttcatatca gcttttgtgc tcttgtcaaa gcgtgttctc ggggcgacat acacctcaca
     5821 accgatgatc ggctttattc cgttctcgat gcattcgtta taaaaatcga cacagccgta
     5881 cataacgccg tggtcggtca gtgcaacggc agtctgtccc atttccttta cacgccgaac
     5941 aagccctctt atacgacaag cgccgtcaag aaggctgtat tcactatgaa cgtgtaaatg
     6001 tacaaatccg ctcactttta ttctccccgg ttttcttctc ctctgagtgt gtccgctata
     6061 aggcttatct ttttcagcct gtgattgaca ccgctcctgc ttatcggcgg ttcaagcatt
     6121 ttcccgatat cgctcagcga caattcaagt gcggtaagac gtatct
//
LOCUS       DAWAOG010000050         6155 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1734_length_6155_cov_2.321475, whole genome shotgun sequence.
ACCESSION   DAWAOG010000050 DAWAOG010000000
VERSION     DAWAOG010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1734_length_6155_cov_2.321475"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3717..3726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4907..4916
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1684 a         1321 c         1517 g         1613 t
ORIGIN      
        1 tgtatactac acgataatct atattatgct cacgcaacat atcgaaaagc ggagcataca
       61 tttcaagaat ttgtcctgcg gctttggcgt taagccacac actctcgttt tccgaacagt
      121 cgggcggtgt cgggagcttg tcaaatacag gcatcatacg cttgatgatc ttctgctcgc
      181 ggtttgtaag gatacgtctg tggaatatca gcttcataaa ggcgactata tcgtattcac
      241 gttctttata cggtacgaac acacggatat atcttgcata aggttcgccg tcagagcgat
      301 gatcgtacag catatccggg cgttttttgc gggtcacttc aagtgcggtt tcttcctgcc
      361 acttcgcaac ggcatcgtgc gcgcctgtaa gcagaacctc aggcacggct ctgtcgtgcc
      421 aaacctcggg acgggtatac tgcgggtgct cgagaagccc gttatagtgg ctctctatgc
      481 tgtaagcatc gctgttaggg agcaccccgt cctgaagcct tgctatcgca tcggtaagta
      541 taagcgccgg gagctctcca cccgtcagta catagtcacc gacagatatc tcctcggtgc
      601 aaagttcgtc aagtacacgc tgatccacac cctcataatg tccgcagagt attatgagat
      661 tatcctcggc ggcaagctcc tttactatgc cttgcgtcag tactttgccc tgaggcgaca
      721 tatagactat gcgcgcagaa gagccgcttt cctgctttat tgcggttata cagtcgtata
      781 tcggctgtgc ctgcataacc atgccagtgc cgccgccgta aggcttatcg tcaacgcggt
      841 tgtgcttgtt gccggcatag tcgcggatat tgtggcagtg tatatctata tgacctgctt
      901 ttcttcctct gccgactatg ctttccgaca ggtacgattc gcacatatcg ggaaagaggg
      961 tagctatatc aatccttatc ataggttctc cgtttttatt cttcgtcaaa caagccgtca
     1021 agcggtctta taacgagata tccgtcgtct atattttttt caagcaggac ctcgggaatt
     1081 gcggggaaca taagctcttt accctccggg gttttaagct catagacatc tgccgcgccg
     1141 tgctgaaaga tatccgttac cttaccgtaa accttgccgc tgtctgcgtc acgcacttca
     1201 agtccgatga gatcctgtat gaagttcacg ccctcgggaa gctccacatc gtcacgatcc
     1261 atataaagca ttttgccgac tatggccttt gcccgctcaa cactgtctat gccctcaagc
     1321 ttcagcactg caagcgtttt gtgcggacgt gccttttcga cctcgagctc ctgcttgtcc
     1381 ttgcctaaaa acagcctgtc aaagctgcat ataacgtctg cgctgtcgca gtagtactgc
     1441 actctgacct cgccgccgag cccctgcgtt gctactatct tacctatttc aagatacttt
     1501 tttctcatat tttcctcatt tctctgttat gtcaagaata tcagaaggct tatgtgcaat
     1561 atattttgca ccgttatcgc aaagctcctt ttcggtgcgg aagccccaca gaacgccgca
     1621 cggggtaaag cctgcattta cggccgtctg catatcaacg ccgctgtcgc ctatgtaaag
     1681 tacttctgac ggctgtacgc cgagcttttc cgctacgtca agcgcgccgt ccgggcacgg
     1741 ctttgccttt atcctgtcat caagtcctct gatatagtcg aacacgccgt caccgaggaa
     1801 gtgcggaact acctgctgtg ctatgttatg cgctttgttg gtaatcacgc caagcttcat
     1861 acctttttcg cgggcggagc tcaaaagctc gggtatgccg tcataagcgg cggtcttgtc
     1921 ctcgctgtga agagcataat attccataaa catggcaagc gcctgttcct tatattcctg
     1981 cctgtcggac ggcagacagc gttctataag cttcggtata ccgttgccga caaaatattt
     2041 atattcatcg attgtgtggg taggaaagcc taatttgcac aaaatgtgat ttgcgctgtc
     2101 gccaaggtca tcgattgtgt tcagaagtgt accgtcaagg tcaaaaataa gtgccttata
     2161 tctcatagaa atacctcttt tcttgctact atctataata ttatatagct taacactgtt
     2221 tgtgtcaacc gagctgataa attaaaattt gaaattttgc acaaaaagtt attgattaag
     2281 agctgttatt atgctaaaat cggtacatac agcaagaaat gctgtatgta gaatattgca
     2341 caaaacggca cgaaaggaaa aagctatgag cacaaaaatg tacgtaaacg gcagtgacag
     2401 aaagggacat ataaacccgg agatctacgg tcatttttcg gagcatctgg gcagatgtat
     2461 ctataacggt atctatgtcg gggaggacag cagtatcccg aatactaacg gtataagaaa
     2521 cgatatagtt gaggctttta aaaatattaa gatgcctgtc ctgcgctggc cgggcggatg
     2581 ctttgcggac gaatatcact ggaaggacgg aatcggagaa aagtcacagc gcaagaagat
     2641 gataaatact cactggggcg gagtgacaga ggacaacagc ttcggcacgc atgagttctt
     2701 tgacttctgc gagatggtag gctgtgagcc gtatctgtcg ggcaatctcg gctcgggtac
     2761 tgtgcaggaa atgtcagagt ggatagaata tatcacctcg gacaacgatt cgcctatgac
     2821 gcaattaaga aagaagaacg gcagggaaaa gccgtggaag ctgaaatatc tcggagtcgg
     2881 aaacgagagc tggggctgtg gaggaaatat gtcgcccgag cagtattcag cactttacag
     2941 gcagtatcag acgtattgca gaaactacgg ggcaaatcag ctttacaagg tcgcctgcgg
     3001 accgagtgcg gatgattata actggacgaa ggttataaca gaaaatatca agccgtgtca
     3061 cactaatgcg gtgagcctgc actactatac tctgcctacg ggaaactggg gggataaggg
     3121 caacgctacc ggattttctg aggacgaata ctataagacg ctggaaaaaa cgtggtatat
     3181 ggaaacgctt gtcaagcgtc attgtgatat tcttaacgta aataatcccg aaaataacat
     3241 aaaactggtt gtagatgagt ggggaacgtg gtataatgta gaggaaggca ctaatccggg
     3301 atttttattc cagcagaata caatgcgtga cgctatggtg gcatcgatca atcttaatat
     3361 attcaacagc cactgtgaca ggatatctat ggcaaatctt gcgcaggcgg taaatgtgtt
     3421 gcaggcgctt attcttaccg aaggcgaaag aatgatactg acacctacat atcatgtgtt
     3481 tgatctgtat aagaatcatc agaatgcaga gctgtgctac agcagtatag cagatgaaag
     3541 gcaggaggga tatgaccttc ctgttatatc gcagagcgta tcggtaaact gcgacaacga
     3601 aatgacgata acggcttcaa actgctcgct ttcggaaagc aagactattg aagcggacat
     3661 atgcggattt gagttcaatt ctgtcagggc aaggctgctg agcggcaatg caggtgnnnn
     3721 nnnnnncgcg gtatcacatt cacgcttccg ccgtgcagtg tagcggagat aatccttgaa
     3781 tgaatgtctg caaaagatgt ctgctgagcg cgatgagtgc cgaggcgttc cggaatatca
     3841 aagagaatgt agagcttatt cccgatgata aaaaagccga tgaagcgctg tacaaaaaac
     3901 gtcttgaaat atgtacggag tgcgactgcc ttataaatgg tatgtgcagt aagtgcggtt
     3961 gttttgtgga gatgagggcg gcttatgctg tcagccgatg tccgcacgaa aacaagctct
     4021 ggtaagtgaa aggaaaaatg ttatggctaa tatgaaaaag cttcagcgcg attacggcga
     4081 tgttatctgg acaggcaaaa agtgcattat gggtatgcct atctcattta caagatatat
     4141 acttacatca agtgtgcttt atacgaaggt cggatttctg aatatcaagg aagatgaaat
     4201 agagctgtac aagatagttg ataaaaccat gaagctgtcg atcggtcaga gaatggtcgg
     4261 ttgcggtact atcgtggtca catcaaggga ctgcgacact ccgaacaagg agctgaagtc
     4321 tgtcaagcag cctagagagg taaagaaaat acttgacgag gcggtaaagc ttgagcgcga
     4381 caagtatatg gtaagaggca gagatatggt cggcgcaaac ctgcatgaac acggcgatat
     4441 gggtgaaact aatctgcata gcgaagattt tgacgaaatg gcggatatgt aattggataa
     4501 gataaaggaa atgttaaaaa tggctctgca cccatcacag tggaaggagc tttttacaag
     4561 ataccgtgaa ataataatgt atgtcatatt cggtgcactg acaacgcttg tatcaatagt
     4621 gacgtacttt ataatacgct gtatattccc gggcgaggag agcgtacctg cgtttttgaa
     4681 atggacatat cggctcaatt tcagcggcgg agattcgtca accgtactgc cgaatatcat
     4741 ttcatggata gtatcggtca catttgcgta tatcacaaac aggatctggg tgtttcaaag
     4801 caaggtaaag ggctttggca agaatatagt gcaggcgctg tcattctatg cggcaaggct
     4861 gttcacgctg tttgttgatc ttataataat gttcctgctt gtaaatnnnn nnnnnncgaa
     4921 tattgagaac gggctttatg aattctgcgc aaaggtgttc tcaaatatcg tagtgcttgt
     4981 gctgaactat attttcagca aggtgttcgt attcagaaaa gaaaagcaga aataaacagt
     5041 aaaaatcaca gcgtgtattc actgctgtga ttttgtttgt tatgaaactt cgggctaaag
     5101 gagcttttat gaaagaaaaa gaatatgtgt tttcgtattc tcttgacaac aagcgttatc
     5161 atactctgaa cttccataac aaaaccgttt acggcggcag ggtaggtaag gctgttatag
     5221 atgcgggctt tacctgtcct aataaggacg gaactaaggg tacgggcggc tgtatctact
     5281 gtatggacgg aagcggatat ttcaccgcgg cacatggcgg caatatctat gacagcgtac
     5341 agcgccagct ttcagccgaa aaggagcgta tctttaaaaa gtaccccgac agtatgataa
     5401 ttgcatattt tcaggctaat accaacacct atgcgcctgt tgaaacgctc aggatcgcgt
     5461 ttactgcggc tcttgatttc ggcgtaacag gtatatcgat aggtacaagg gcggactgcc
     5521 tgcccgatga tgttatcggg ttgctttcac aactgaacag caagacagac cttactgtag
     5581 agcttggctt gcagactgtt cacgacagaa ccgccgccgt tattaaccga tgctatgact
     5641 accgcgagtt cctttccggc tatgaaaagc tgaaaagcgc tgatatccgt acatgtctgc
     5701 atataattaa cggaccgccc ggggaaacaa aggatgatat gcttgacacg gcaagagagg
     5761 tcgcgcgact tcacccggat gctgtcaaaa tacatctact tcatgtcaac agtaacacag
     5821 ctctggaaag aatgtacctc ggcggagagt atagcccgct tacccgcgat gagtatattg
     5881 atatagtgat atcacagctt gaatacttgc cgcaggatat agtcatagag cgcattacag
     5941 gtgatgccga caagaaatat cttgttgcac cgaagtggag cgcggataag attgccgtac
     6001 tcggcggaat agacaagcaa atgaagctga ggggaactta tcaggggata aaagctgatt
     6061 ttatgtgatt ttatatctga atataagtga aaccgcagta tcgggtcagt ttttgatact
     6121 gcggtttgcg gtgctttttc tgctatattg tgcta
//
LOCUS       DAWAOG010000051         6112 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1746_length_6112_cov_2.451048, whole genome shotgun sequence.
ACCESSION   DAWAOG010000051 DAWAOG010000000
VERSION     DAWAOG010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1746_length_6112_cov_2.451048"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5434..5443
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1827 a         1295 c         1549 g         1431 t
ORIGIN      
        1 gtacgtcttg cgatatttgc aagaaaggta cgtccgggtg agagcactta tgttgacgtt
       61 gataaggcaa cggtaaatac ctatgtcaag cgctggcagt gggtatatga taacatcggc
      121 agcggctatg actggtcgca gagcaactgg gacagaagaa agctgctcag ctatactgcc
      181 gaggacgcac agagggacgg ttatacattc agtgatgata taaacgatca ggacggcgta
      241 tatgattata acaacggcga caattacgat tattattgat gtatggaatc aaattgtaaa
      301 cggaaggcgg tgcggcgttg gaagaggaac acaaggaaga gctgtctgtt acgccacaag
      361 ctctgaaaca gcctgtggta agaggcggaa tgatatgggt cgcactaggt gcctgcgcgg
      421 tatggtttat agcagtcggc gttatgttga tactaaagct taccgaagcg ccgcttcctc
      481 cgaatatgat agactatacc tgtacgctga acgtggatat acctgccgag cctgccgtgc
      541 ccgaaaagaa aaagtccgga atactgccgg aatacaggga actgtatgag caaaaccatg
      601 atatggtcgg ctggctgaag atagagggta cgaatgtcga ctatcccgta atgcagagca
      661 agggctttga tcctacaaag ccgatagatt acgatgtggt ttacgaaaaa aatatgtact
      721 atctgaagca tgacttcaac aaggagtaca gctattccgg aagcgtaacg gcagagtact
      781 gcgcacacat cggaagcgac taccgctcgg ctaatatcat tctttacggg cacaatatgg
      841 caaacggaac atttttccgg gatgtaaata agtttgatgc cttgtattac ggcagagaaa
      901 tgtacgacga gcatccggtg atacagtttg ataccatata cgaaaaggga ttgtacaaga
      961 tattcgccat gatgcaggta aatgtagagg aagaggacgg cgaggtcttt tattacaccg
     1021 ccacgtttga tttcaaaaat aagcaggaat ttatcgatta ttatgcgcaa gtgctggatc
     1081 gcagttttat cttcacgccg caggttgatc ttaagtacgg cgatgaagtg atagcgctgt
     1141 cgacctgtga ttttcctttc ggaaagagca agagcataag atatgttctt tttgcaagaa
     1201 gagtgaggga aggcgaagat ccgactgttg acgttacaaa aacagtgata aatcccgacc
     1261 ctctctatta cgattattgg tacagcgttt acggcggttc gtggggcgga cgcaaatggg
     1321 atcccgcgct tctgaaaggc tataaggcgc agaagaacca ctgaaaaata aaacgaaagc
     1381 acaaagcttc cgacaataca aaaacacaag gaaggttatg taatgcctga ttataaatct
     1441 gtcatagtat catcaaacaa gaataagaaa aagaagaaaa agaaaaagga aaacgcattc
     1501 gttgcgatat tcaaaggcct tataccgtgg aagggcgaca gtataggcga ggtaataagg
     1561 aagatcgtat ttctggtgtc gatagttgca ctgtgtgttg cggtgttgat aataatagac
     1621 acatatctct ttaagcccaa gcgcgaaagc gccgattatc agagcagtat attacagctc
     1681 ggcgagcatg aaccgacagc cgaggagatc aaaaagctcc ccgaaaaggc tataaacacc
     1741 gaatatgcgc cgttttatgc aataaacaat gattttgtgg gctggctcag cataaacggt
     1801 ataaatgtca actatcctgt agtacagggc aaagacaata ctcattatct ggaaactgat
     1861 ttctacggaa attacagcaa aaacggaact atatttgccg attacgaaaa cgagttcaag
     1921 aacacgggag aaatgtctgc aaatacgata ttgtacggtc ataatctgcg taccgaaaat
     1981 ttctttgccg aaataaccga gtacagaaga gttgatcttg acaagagcct tgagttttta
     2041 aaggctcacc ctgttatcga attcaatacg ctgtatgaaa aggcaaagta caagatattc
     2101 tcggtaatgc tgctgaacac aagagaagag tacggcgatg tgttcaacta tcccgcgctt
     2161 ctcaattttg caagcaggga cgagtttaat tacttcacat ccgaatgtct tgacagaagt
     2221 gaatttttta cgggcgttga tatgaagtac ggcgataagt tccttacgct gtcgacctgc
     2281 gagtttgagg tcggtcttta tgatatgcgt atcgtaattg ttgcaagaaa ggtgcgcgag
     2341 ggcgagtcgg acgagttcac ggcggacgag atagcaagct ttaagcgtaa tcccgatccc
     2401 aagctgtgta aaacgatgcg cgagctttat tactatgatg ccgagtggag cggaaggtat
     2461 tgggatcctg cgtggataga gggcttcaag aaggacagct catttatcac ggacgacagc
     2521 gataaagata cggataatac aactgaggcg gagagcaccg aataataact gttaaagtga
     2581 gggagtggta cagatgcaga gagcatctat aggaaagatt ttgctggcgc ttgtcatagc
     2641 ctgtatatat atatacctgt ttggaatagt ccaggtagtg catttaagcg cggacaatga
     2701 cagggagata gaagtggcta tacctgaggt gactgcttct gcacagacgc agaaagcccc
     2761 cgaaaccaca acgataaagc ttcagagcga caaaaagccg acgctcggcg accgcaaggc
     2821 gcagacaaca gccgcgacaa cggcgcaaac aactacgaca actataaaaa aacgtgtacc
     2881 tgcacctgca gcaacatcga tcgataatac agttgtagtt cccgaaacaa caacgatagc
     2941 tgaaactccc gcacctgaaa ccaaagcacc cgaaactaca acgacagcgc ctgaaactca
     3001 gtcggtcgtt cctgtgcctg aaacaacggt cgcgccggaa actacagcta caccgcaaac
     3061 aactcctgcg ccggaaacca cagccgccga agaaccgaca cccgataaca gcggagcgga
     3121 acagttcacg gtgtatgacc aggtaacggg aacgtataaa acaggtagcg caagagatat
     3181 aatcgcgggg gcggtagtcg gagagatatg gaacgagttc cccgatgagg cggttaaagc
     3241 tcaggcggta gcggaatata catatatcaa aaagcagaat gagatgggac tgcgtcccac
     3301 tgtatcgctt aagacggata tctatgacag gatatataag cttgttgatg aggtgctcgg
     3361 cgaagcgatc tactataatg gcgagatgat acagagcgta ttctatgcat cgtcctgcgg
     3421 atatacaaac agtgccgaga acgtatggaa tgtttactac ccatatctcg tgagcgttga
     3481 ttgtccgctg gacaagacga ccgaccccaa ctggggaagc actgacagct attcttcgga
     3541 ctatatgagg aatgcggtac agagcacgct cggaattacg cttacaggcg acccgtcaac
     3601 gtggttcaag ataaattcaa cacttgataa cagagagcac ggctgggtaa catcggtttc
     3661 tgtcggaggt atgacaaagg ctaacggcaa gacgatcgac ggcagagtga taagagaaac
     3721 agtgctcgga tactcgctca agagcgccgc ttttgagctg aagtatgacg caggcagtga
     3781 caagttcatc ttcacgacct acggtcacgg tcacggtgtg ggtatgtcgc agtatggcgc
     3841 aaaggctctt gcggaaaacg gctacagcta taagcagatc ttacagcact atttcacagg
     3901 tgtagagata cactgagaat gctttgaaag ggcaccgcaa ggtgtccttt atttctgcgg
     3961 ttttgcttgc attatccgaa attatatgct atactttaat ttatatggtt aaaatacgaa
     4021 cgaagatgta agaaaaagta aatgtgtaac aagaaaggaa acaaatatga taacagtatc
     4081 ggacgttagt ctttcattcg gcggacagat gttgttcaag gacgtaaacc tgctgttcac
     4141 agccggtaac tgctacggtg ttatcggcgc aaacggcgcg ggtaagtcca cattcttaaa
     4201 gatactttgc ggagcgctcg agcccacaac aggtacagtc acagtgcagg aaggtctgcg
     4261 tatgtccgtg cttaagcagg atcactacgc atatgacgaa tatacggtcc aggataccgt
     4321 aataatgggc aatccccgct tgtatgaggt aatgaagcag aaggacgctc tttatgcgaa
     4381 ggaggacttc accgaggagg acggcaatct cgcaagtgag cttgagggcg aatttgccga
     4441 gcttggcggc tgggaggctg aaagcgacgc atcaaagctt gtacagggtc ttggtcttga
     4501 cgagagcatt atgtattctc agatgagcag tcttgcaggt aatgaaaagg tcaaggtact
     4561 gctcgcacag gcacttttcg gaaaccccga cattatcctc atggacgagc ctaccaacca
     4621 ccttgatatt gacgctgtgg cttggcttga ggacttcctt gcggagtatt tcggtacggt
     4681 catagtcgta tcgcacgacc gtcacttcct taataatgtt tgtacccaca cggtagacat
     4741 cgactacaca aagataaaga tgtatgtcgg caactacgag ttctggtatg aatcttcaca
     4801 gcttatccag aagatgttga agcagcagaa caagaagaac gaggagaaga taaaggagtt
     4861 acagagcttt atccagcgct tctccgccaa caagtcaaag tcgaagcagg ctacatcgag
     4921 aaagaagctt atcgacaagc tgacagtaga agagctcccc gcgtcaagca gacgttatcc
     4981 gttcatcagc tttgatatgg agcgtgagat aggcaaggat attttgcagg tcgaagggct
     5041 ttcaaaaacg gttgacggcg ttaaggtgct cgaccatgta agctttactg tcggcagaaa
     5101 cgacaagatc gcttttgtag gcgacaacga gatcgcagtt actacactgt tcaagatact
     5161 gatgggtgag gaagagccgg acgagggctt tatcaagtgg ggaagcaccg caagcgtaag
     5221 ctactttcct caggacaaca gcgcgttctt caacaactgc aagctgtcca tccttgactg
     5281 gataagacag tattcaaagg acgaaaccga aacctatctg cgcggcttct taggtcgtat
     5341 gctgttctcg ggtgacgata tattcaagag cgttgacgtg ttatccggcg gtgagaaggt
     5401 acgttgtatg ttctcgcgta tgatgctgtt ccannnnnnn nnnagatagt acagacggtc
     5461 gcaaaccgta taatagaaat tgctgacaag ggctgctacg acagacaggg cacatatgaa
     5521 gaatatgttg actggagaaa ggcaaaccac ggcggcagct tcacactctg ataaaacagg
     5581 atatataaca aggcggactg tcctttttcg ggcagtccgc cgttttattt aatccttgct
     5641 gtaccaccag ctgccgtaat aatcgccgct gtttatcata attgatgtat tcttctcgtc
     5701 atctctgtgc caatacaatg cggcaacggt actgccgtta tctttttggc taagagtata
     5761 gccgtctgcc gatgcggtaa tatcatttat ctcaaatgtc tgctcaccga gctttaccgt
     5821 tccgttcgca ctgcacagtc ccgcgttatt cacggtaaca gttatgtcga taacggtatc
     5881 aagctttgtg ccctccggca tatcaccctg aacctcggca tcggcgatat gtgtctttac
     5941 aaacggattt tcaatatgta tcgtttcatt gcgcggtaaa aacagtataa gtcctgctac
     6001 aaccgctccc agtacgacta aaacacttat aataattaca atcgtctttt tcatcattgc
     6061 ctccggtttg atcactcagc cgagctttcc ttgagatact cgggaataaa ct
//
LOCUS       DAWAOG010000052         6107 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1750_length_6107_cov_2.703900, whole genome shotgun sequence.
ACCESSION   DAWAOG010000052 DAWAOG010000000
VERSION     DAWAOG010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1750_length_6107_cov_2.703900"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4535..4544
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5462..5544
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1491 a         1624 c         1079 g         1820 t
ORIGIN      
        1 acggatttgt ccttgccgca acagttgcac tgtatgcgcc gtaaaccgca gtcttaccac
       61 tcatcttgta ggctcttact ctgaacttat acacagtcga agcctttagt cctgcttttc
      121 tgaacgttgt cgtgctgttg ttggttatct tagcaactct tacccacttg tcgccctgat
      181 acatctcaac gatatatccg tcagcagaag catttttact ccagttgatt ctgagcgcgt
      241 cagctgctct gccggtaagc tttgcgccgg ttacttttga gagtgtgggt aacttgtcaa
      301 gaagtacata atcaaatccg ttgtctatag cgtacttttc accctttgtg ccgctgtagc
      361 agtagatttt gaagttgtct atcttcttgt agccgttatc attatagtaa ccgaaagcat
      421 gattgcgtat acgtgccaca ctgctaggaa tagtaatact ggtaaaattc atacaatcaa
      481 ggaaaacctc ataaccaatg aactctacac tgtccggtat cgttataccg gtaagacttg
      541 tgcaaccgct gaaagcacta tcgccgatac cggtaacact gtcaggtatc gttatatttg
      601 taaggtttgt gcaaccactg aaagcataaa cgcccatact cgtcacactg tcaggcatgt
      661 tataattttt atcttttttt ccggcaggat aacatattat agcagtttta tctttattgt
      721 acagaacgcc attcacagaa gcatattttt gatttccggt tgcaacattt atcgccgtaa
      781 ggcttgtgca acctctgaaa gcacaataac ctgtttctgt cacgctgctc ggtatcgtta
      841 tgcttgcaag acttgtgcaa tctttaaaag ccaaatttcc tatacttgtc acaccgtccg
      901 gtatcgttat acttgtaagg cttgtgcaac cgctgaaagc atcatcgggt ataaatgtca
      961 cactgttcgg tatcgttata cttgtaagac tcgtacaacc gctgaaagca ctactgccta
     1021 tccacgtcac accatcaagt atctcataat ttttatcttt ttttccggca ggataacata
     1081 ttatatcagt tttatcttta ttgtacagaa ctccgttcac tgaaacataa ttctgatttc
     1141 ctgttgcaac atttatcgta gtaagacttg tacagccttc aaaagcttca ccggttatca
     1201 ttctcacact atcaggtatc gttatacttg tcagacttga acaaccgtta aaaactccaa
     1261 catatatacc atccacacta tttggtatcg ttataccggt aagacttttg caatcttcaa
     1321 acgtccaatt tcctagactt ctcaccccgt ccggtatcgt tatgtttgta aggcttgcac
     1381 aacttctgaa agtacaatcg tctatatccg taacactgtc cggtatcgtt atgctcgcaa
     1441 ggcttgagca accttcaaaa gctctgctac ctatactcgt cacaccgtcc ggtatcgtta
     1501 tgcttgtaag acttgcgcaa cttctgaaag caccaccgtc tatactcgtc acactatctg
     1561 gtatcttata acttttatct tttttcccag caggataacg cattatagta gttttatctt
     1621 tattgtacag aactccgttc acagaagcat atttttgatt tccggttgca acatttatcg
     1681 ctgtaaggct tgtgcaaccg ctgaaagcac aataacctgt ttctgccaca ctgctcggta
     1741 ttgttacact tgcgagactt gtgcaatctt caaaagccca atttcctata cttgtcacac
     1801 tgcttggtat agttatactt gaaaggtttg cgcaacctcc gaaagcacta tcgcctatac
     1861 tcgtgatact gttcggtatc gcaacacttt taagacttgt gcaaccgctg aaagcatcat
     1921 cagatatata tgtcacactg ttcggtatcg ttatacttgt aaggcttgcg caaccttcga
     1981 aagcactatc gcctatacgc gtgatactgt tcggtatcgt tatactttta aggtttgtgc
     2041 aaccttcaaa agctctcctg cctatactcg tcacaccatc aggtatctta taatttttat
     2101 ctttttttcc agcaggataa catattaaaa cagttttatc tttattgtac agaacgccat
     2161 tcactggaac ataattctga ttttccgtag caacatttat cgctgtaaga cttgtacaac
     2221 cgttgaaaac gttatagctt atcttcgtca cacttttagg tatcgttaca cttataagac
     2281 tttcgcaacc gctgaaagca ccactgccta tctctttcac actgtccggt atcgttatac
     2341 ttttaaggct ttcgcaaccg ctgaaagcac caccgcctat ctctttcaca ctgtccggta
     2401 tcgttatact tttaaggctt tcgcaacctc tgaaagcaca accgtcaata cttgtcacac
     2461 tgcccggtat cgttacgctt ttaagacttg tgcagtgttc aaaagtgtaa ttgcatatct
     2521 ctgtcacacc gtccggtatc gttatacttt taaggcttgt gcaataccta aaagcataac
     2581 tgtatatact tgtcacactg tccggtattg ttatacttgt aatattacta cagtattcga
     2641 aagcattagc acctatcctc gtaacactct tcccatcaat ttcagcagga atatcaacct
     2701 tttccgcact tccgtaatac cttgcgatct ccaccgtacc gtcatcaagc actttgtaga
     2761 ataaatcttc tccatacatc tccgccaccg cgcacaccgc acagatcaca gccatcgccg
     2821 caaaggtcac aagccccaac agccactttt taactttcat attactctct cctttttgat
     2881 gaaattagaa tataacacaa aaaggcatac cccacccctc gtggactatg ccttaattac
     2941 catataaaat tttacacaga gatattgtgc attacttaca gaggcacttt tatagagccg
     3001 tcctctacac aaattgtcat tcgctgtttt gcgcatatct cccacccttt atttaatcga
     3061 ccttaacttt atactttcga tagcattagt atatcattat tgctataaac ttgtcaagcg
     3121 gttttggtta ttaaatgcta cttcaagtac atttgtttag cttcacacaa aagctactac
     3181 agcactccac catttctgcc atcacctcca accctcacta tccctcgccc cgcactacca
     3241 catgcttggc ttatgaacgc tctgttggtt ttgcatcctt gcaaaactcc gggcgttcaa
     3301 tatcgggcat ccttgcccgt cccttgccaa agggagctgg cttgcggcaa ggcttgctgg
     3361 tttcagatac cgccatactc aacgataccc gcaacgctca aatctcacaa accctatctg
     3421 ccgtcaagac tgagggatcg cctgcaccga acttttccgc ttccgccatc acctccgctg
     3481 acaatccctc agtcacagtg acgaaaagct ttgttctaca tcaaaacaac tttaatctga
     3541 tatataaaac aataccgaaa atgttatacc gcactgtgac agctcccttt acacaaggga
     3601 gccgggtgcg atagttcttt gccaaacttt catcagatta taatatcaca tttagtttta
     3661 acagaagcac aatctctatt ttcgcactgt cccaaaatca atatacgcgc cgcaggcgca
     3721 accacaatta ttcattattc atcattcatt atcttcacta ccgctcctca acgcttatct
     3781 catctcttgt aacggtagcc tcatgattat agaacttttt aagctcattt accgacatat
     3841 agttgcctgt aagctcgccc ttctcgttga taagatatct gccgttccac ggagcgaccc
     3901 atagccacac agcattatcc ctcgccataa tatcggggtc cggagctttc gcgcactccg
     3961 tcagcgcaag catacgcttg ttgagcgaga gcgcatcagt agcatacatc gccgcaagat
     4021 gagccgtatc ggtattgtcg tacacatcac agcctattat atcgacatag tcgtcaccgg
     4081 ggaacatctg cgggtcaatg tccgcagtat acacccacag cagatttgac aatccataca
     4141 gctcgtcaaa ccgtctgtgc atagtctgcc acagccactt atatgttttg ttgtccttct
     4201 cccaccagta ccgacccgca ctgtctgtag gaagaggagt aaacagcacc gtaacgccgc
     4261 tgtccttaag caccgtgagc tgttttgcca cagcgtccat atctgacagc accgcatagc
     4321 actcctcgga tatagtttca tctttaagga gaagcgccag cgactcctca ttcatgaccg
     4381 caatatcttc atagtcgctc atgaccgaat ttacatcaaa accgctctca ccaagataga
     4441 acgttgtctt tcctatcggc gcataccagt accatgtata cgacactatg ccgccgttcc
     4501 ttgcccactc ctgcgcaagc tgtatatcaa tattnnnnnn nnnngctctg tattcacgtc
     4561 cttgccggat atccacgttc ccgcatcact gtacctttta tcacttgccc tttcggtatt
     4621 ttttaccgtt atcttatcga tcgaaaccgt atttactacc gtctggagcg tcactctgtg
     4681 ctttccctgc gtcagataca cggggcacag cgaaatgctc tcaaacgtat cgctctgcac
     4741 cacgttatat gcgccgtaca gctcgccgtt tatgcgctta tatgctccgt tctcactgtc
     4801 gatctgcttt tcaccgtcaa ccgtcagcgt tatactgccg ccttctttcg acagcgcggt
     4861 tatcgaaata tcgtaaaact gcgtggacga aacctcaagc tcaaacgaca cctgatcata
     4921 cgcttctatc gttatatatc cgtcgccgct gtatttctta ctgtctgata taagctttgt
     4981 gtcaggcagc ttttccgctt caagcgtata gctgaatgcc gatctgttca gcttatattc
     5041 cattgtcgtg ctctcggtga cgatattcgc ctcaggcacg gagctttcct gcgccgcaga
     5101 ggtttccccg ctttgtgccg aagagatatc cgagtagtct atttccgtac agcctgctaa
     5161 aaccactgcc gcaattaccg ccgacagcgc ccgcaccgcc gttgttttcc ttatgtgcaa
     5221 gtcagtcctc ctgcttaatg accactatat cgcccgactg tatcttgtcc gtgaacttcg
     5281 cactgccgcc gtttacgctg agtacaagtc tgcgtgtggg attttcaatg tcgatatccg
     5341 ctctttcaaa caggtcgata agcatataag gtgtcttgct gtccttctcg ggaagatcgg
     5401 tcggaacgcc gttgaatatt attgatacaa aaccgccctg ttctccggca ggctcgggcc
     5461 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnntttatc aaagcccagt atatcaccgt ttttgagaat
     5581 atagctttcg ggctgaacct gtccgtttac atatacctgc gccgtttcgg tatctccgcc
     5641 gtactgcatc aggaatgcac cgagtgttct cgcgtcatgt atttctatgt tgtcgagcgg
     5701 ctgtatctca tagtccccgt cgacctccct gccgtttaca tatgcgcgct ttcccgcaac
     5761 cgcattttca ccgcagaata tgacccccgc cttggaaaga tcatctgcat agtcacggat
     5821 aaacgcgtgt gcattctcac cgttcaccgc aggcacaaga atgatatcat caccctgctt
     5881 tatctttgtc gtaagtgcgg cggacttgcc gttcaccgta attatcgcag gagtgaacgc
     5941 agtaccctta agtatctttg tttcaccgtt taatgtgaac ttaagtcccg cgccgctgtg
     6001 tcccattatc tgggatgtct tgtatcccgc cgagcccagc agctgaaaca cgctcagcga
     6061 cttcgtatcg aacactctca cctactcgcc gttgagcgtc acggtcg
//
LOCUS       DAWAOG010000053         6074 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1764_length_6074_cov_2.341253, whole genome shotgun sequence.
ACCESSION   DAWAOG010000053 DAWAOG010000000
VERSION     DAWAOG010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1764_length_6074_cov_2.341253"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1669 a         1396 c         1501 g         1508 t
ORIGIN      
        1 gcgtatatga cagtaccttt accggttttg aggtgttgtc tgacattccg aacatttact
       61 ttgaaagaag cttctttact ctctgcatag cttcagccgt gttttcgtga gtgccgaatg
      121 cagtgagtct gaaccagccg tcgccgttct tgccgaaacc tgcgccggga gtgccgacaa
      181 cctcagcttc gtttaaaagc ttgtcgaaga atgtccagct gtccattccg tcggggcatt
      241 tgaaccagat gtacgggctg ttcttgccgc ctgtatactt tacgccaagc tcgtcaaacg
      301 tttcgctcat aacctttgcg ttttgcatat agtaagctat agtagctctt gtctgcttgt
      361 agccctcttc tgtgaataca gcctctgcgg cacgctgaac gggatatgaa acgccgttga
      421 acttgcttcc ctgtcttctg caccataact gcataaagct tatctcttcg cctgtgggag
      481 tttcaagcat aagctcttcg gggataacgg tataaccgca tcttgtaccg gtaaagcctg
      541 ctgtcttgga aagtgagcat atctctatag cgcactttct tgctccttct atctggaaaa
      601 tcgagcgggg gagtgtttca tcggagatga aagcttcgta agccgcatcg aaaatgataa
      661 cggatctgtt ttttatggca aagtcgaccc ataccttgag ctgttccttt gtatataccg
      721 agcctgtggg gttgttgggt gagcaaagat atataagacc gggtttcatg gattcgtcgg
      781 gcatagcggc aaagccgttt tcttccgtgg agtttacata gtgaacttct cttccaccca
      841 ttacgtttga gtcaacatat acggggtatg cgggatctgt tacaataacg tcctcgtctt
      901 cgctgaagat gtcgccgatg ttgccgcagt cgctctttgc gccgtcgctt atcagcacct
      961 cttcgggaga aacggtcacg tcgaagctct tgtagtagcc tgcaaccgct tcacgaagga
     1021 aatcgtagcc ctttccgctg tcctcataac ctctgaatgt ttcctttacg cccatttcgt
     1081 cagcggcctt cttcatagcc tcgactacaa cgggagctaa aggctgtgta acatcgccta
     1141 tacccatctt tatgatcttg ttatcggggt gggcggctgt atactctctt actctcttgc
     1201 ctatttcaat aaaaagatag cttttcttaa gatttaaaaa gcccggattc atctttgcca
     1261 tttgcgttaa gtttcctttc tttctgtaat cgctgtgtcg cgatatcgac acagatatac
     1321 acaatatcat tatatccgct gtaacttcaa aagtcaagaa ataacggtac ttaaaactag
     1381 ccaaataata agggcgtttt acggcaattt tactcaaaat tcagaaaatg cgggcagaac
     1441 tacacttatt taatataatt tctgtgatac atcttgaaat tcctgcgtaa atagtgtata
     1501 attttgaatg tgtaacgttc ccgaaaagtt aatattacgt taatcggaac gtggcagaat
     1561 tacagtaaaa atcggcatat agccgtataa attcccggaa ggaaaagaac agatgttgca
     1621 tataggactt gacgtaggtt caactaccgt taaaattgcg gtgctggacg atgataataa
     1681 tgccgtatac aagaattatc aaaggcatta ttcggatata aaaaagacaa tagcagaggt
     1741 tctcggcgga tgtcttgaaa ccatagacga gaagaatgtg accgttgcgg taacaggctc
     1801 gggcggcatc tcggtttcgc actggctcgg aatacccttt gtacaggagg tttccgcagg
     1861 aacaacggca atacagacat ttatacctca gacggatgta gctatagagc ttggcggcga
     1921 ggacgcaaag attacatatc tcacaggcgg tatcgagcag cgtatgaacg gctcttgtgc
     1981 aggcggtaca ggcgcgttca ttgaccagat ggcttcgctt ctgaatacag atattacggg
     2041 aataaacgag cttgcaaagg actacacgac tatataccca atagcgtcgc gctgcggcgt
     2101 tttcgcaaag tcggatatac agcctctttt aaatgacggc gctaaaaaga gcgatgttgc
     2161 cgcatcaata ttccagtcgg tcgtaaacca gacgataagc gggcttgcct gcggtaagcc
     2221 gataagagga cacgttgctt ttctcggcgg accgcttcac tatctttccg agctcagaaa
     2281 gcgtttcatc gaaacattac agcttgatga tgagcatatc atattcccgc ccgatgcaaa
     2341 ccttttcgtt gccatgggcg cttcccttgc ggaaaaccgc gaggagcttt ctgtggacaa
     2401 gctgagggac gatcttaaag cgcttgctac agtagaggta cacgaggtag aaagacttgc
     2461 acccctgttc aaggacgagc aggagcttaa ggaattctat gacagacacg caaaggctgt
     2521 tattcccgta aaagagctgt ccgaagccga aggaccgtgc tatctcggca tagacgcagg
     2581 ctctaccaca acaaaagcta ctcttatcaa taaagacggc gatatacttt attcacatta
     2641 cggaaacaat atgggcgatc cgctcaaatc cgtaattgag atagtaaagg acatatattc
     2701 aaaaatgcct gaaaaggcat atatagcaaa gtcgaccgcc acaggctatg gcgagcacct
     2761 tatcaaggcg gctctcggtg tggatttcgg cgagatcgaa acaatggcgc actacaaggc
     2821 cgcagagaag attctgcccg gcgttgagtt tatcctcgac ataggcggtc aggatatgaa
     2881 atgtatgcgt gtaaaggacg gggaaataga gagcatactt cttaacgagg cgtggtcctc
     2941 gggttgcggc tcattcatac agaactttgc aaatgcgctc ggtatgcagc ctgaagaatt
     3001 tgcacagata ggcctttccg caaaaagccc ggttgacctc ggttctcgct gtacggtatt
     3061 tatgaacagc cgtgtaaagc aggctcagaa ggagggcgca agcgttgcgg atatatccgc
     3121 aggactttcc tattctgtag taaagaacgc tcttttcaag gttatcaaga tacgcgaccc
     3181 aaagcagatg ggcgaaaaga taatagtaca gggcggtaca ttccttaata actcggtact
     3241 gcgcgcattt gagcttacct gcggacgtga ggtagtacgt ccggataagg caggtcttat
     3301 gggcgcatac ggaagcgcgc ttgtcgctct gtcccgtgac gacagcaagg gcagtacgtt
     3361 agccccgctt gaaaagctcg aaaactttac tattcagaaa accaccgcac gctgcggcag
     3421 atgttcaaac aactgcctgc ttactatcag taagtttgcg gacggaacaa gatacataac
     3481 gaacaaccgc tgtgagcgcg gtgcagggct cggaagcaaa agcaaggagc ttttgcctaa
     3541 cctctttgac tacaagtaca agagattgtt cgactatgag ccgctcgacc ccgaaacagc
     3601 acccagaggt gttgtcggta tcccgcgtgt actcggactt tacgagaact atccgttctg
     3661 gttcacactg ttcacaaagc tcggctacag tgtgaagctg tcgcctaaat caagccgtga
     3721 gatatatgac agaggtatag aaacgatgcc atctgagtcg gtatgttatc ccgcaaagct
     3781 ctctcacggt catataatgt cgcttctgga cgatggcgtg aagttcatat tctaccccgc
     3841 actgccctat gaaatggata acggcggcgg tggagataac cactataact gccccgttgt
     3901 cgcaacatac agcgaggtca taaagaactc cgtacccgag cttcgtgcag acgatgtaac
     3961 atttatgaac ccgtttttac ctatatatga cgaggacaga atgcttgaac gtcttattga
     4021 ggagtttatg ccgctcggca taggcggtgc ggaaataggc gttgcgctca aagccgccta
     4081 tgaagaggac agacgcttca aggacgatat acgttcaaag ggcgaagaag tgctcagaat
     4141 gctcaaggaa aagggcaaga agggtattgt tcttgcgggc agaccttatc atgtagaccc
     4201 tgaaataaac catggtatac ctgagatgat aaacggttac ggctttgcgg tgcttacaga
     4261 ggatagtgta gcgcatcttg gcactgtagt acgtcctatc cgtgtagtag accagtggat
     4321 gtaccacaca agactttatg cggcggctac gcttgtaggt cagacccccg agcttgagct
     4381 tgtacagctc aactcattcg gctgcggact tgacgctatc acaacagacg aggtacagga
     4441 aattctccag ggctacggca gaatgtacac cgtactcaaa atagacgagg taaacaacct
     4501 cggcgcggca agaataagac tgcgttcgct gatatccgta atggaggagc gtgaaagaaa
     4561 cggcataaag cccgtaccga agtacaaggg ctatatccgt cagcctctgt tcacaaagga
     4621 aatgaagaag gactatacga tcatagctcc gcagatgtcg ccgtatcatt tcgaactgct
     4681 tgaacaggca ttccgctatt cgggatacaa tgttgagata cagaagaact attcaaagga
     4741 agtagtagac gagggactga agtacgtcaa caatgacgct tgctatcccg ctatcatcac
     4801 tataggtcag ctcctttatg cgctcaatca cggcaagtac gacaaggata aggtagctgt
     4861 actgataact cagacgggcg gcgcgtgtcg tgctacaaac tatgtcggta tgctgaaaaa
     4921 ggcgctcaag gacgcaggtc ttgacaatgt cccgctgatc tcgcttaacg ttgtaggaat
     4981 ggagaaaaat cccggcttta cactgacact tccgctcgtt taccgcgcgt tcatatcggt
     5041 tatttacggc gatctgctca gccgctgtgt ttaccgtgta cgtccttatg aggctgaaaa
     5101 gggcgcaacg aacaagatgt atgagaagtg gaacgaaaag ctgaaaggtg agataaagag
     5161 cctgtcgctt gcacgtttca acaagaatgt aaaggctatc gttgccgatt ttgattctat
     5221 acccacgctc gacattgaaa agccccgtgt cggtattgtc ggcgagatac tcgtaaaata
     5281 ccaccctgcc gcaaacaaca acgttgtaga gcttgtcgag gctgagggcg cagaggctgt
     5341 agtacccgac cttatgggat tcatctactt tatctgcgac cacgcaaatt cacgtcacga
     5401 gctgcttacc gtttccggcg caagatataa gctgagcaat atggcgataa agctgatcga
     5461 gtttatgcag aagtcgtaca agggtgctat tgagggcacg aagttcggct cgtttatgaa
     5521 gataagcgat atgagaaagc ttgtcaaggg cgttgtatcg acaggtaaca tagcaggtga
     5581 gggctggttc ttatcagcag agatgatcga gcttatccac agcggcgtaa acaatataat
     5641 ctgtatgcag ccgtttgcct gcctgccgaa ccatgttacc ggcaagggca ttatcggaga
     5701 gctgcgcaga caaaatcccg agtcgaatat catagccgtt gactatgatc ccggcgcatc
     5761 ggaggtaaac caggtcaacc gtatcaagct tatgcttacc caggcattca gacagctgcg
     5821 tgagaagaag ctcccgacag aggaaagcat tgtcgcaccc gaggctaagg ttaaaatcaa
     5881 cgataaatat tccgcactcg atgtttaagt gcagttaata accttgtgtt attctgtgaa
     5941 tgtaacataa acccgacaaa aagaaaggca aaattatggc agaaatgaaa ttcagcacaa
     6001 gctcaggtac atcggacagc aacggattta acggtaagaa ggcaggcaag atagcggtta
     6061 tcgctgtcat agcc
//
LOCUS       DAWAOG010000054         5957 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1791_length_5957_cov_2.370722, whole genome shotgun sequence.
ACCESSION   DAWAOG010000054 DAWAOG010000000
VERSION     DAWAOG010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1791_length_5957_cov_2.370722"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1421..1430
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1591 a         1208 c         1617 g         1531 t
ORIGIN      
        1 attcaatgtt gcggatataa tcggcaatta tacggagcgc gcggaaaagc tgacgcgtat
       61 attatggata cttcagatac cggctatggt aatgctggcg ttctatctgt ttatggtatc
      121 tcagctcaat gttgacagag agcgcaacga gattgccgtg ttcaagagcc gtggcgcaag
      181 ctcgtggcag atattcggta tgtatgccgc agagtcgggc atactcggta ttgtaacgct
      241 tgtggtcgca ccgtttatcg gactggtact gtgtcagttc ctcggtgtat cgaacggatt
      301 tctggagttt gtaaaccgta ccggaattgc cgcaaagata accggcatca gcgtactgta
      361 tgcgcttctt gcggtagtgg tattcttcct tactacgatg ataccgatca ttcccgcgtc
      421 aaagcttact atagtacagt acaagcagag caggacaaag gtcgtgaaga tgtcgctgtg
      481 ggaaaagtgc ggcgtcgata ttgtgcttct tgctgtggcg ttcggcttcc tttacttcta
      541 cacaactaat atcacaaatt ctatagctga gggtactttt gaagcgacag gcgagcttga
      601 cccgctgctc ttcatattct caacgcttat gatactgggc ttcggacttc tgtttatcag
      661 aatttatccg tatcttttaa ggcttgttta ttatgtgctg cgcccgttct cgacaccgtc
      721 gcagtatatg gctattacta ctgtttgtcg ttcacagggc ggcaaggagc gttttcttat
      781 gctgttcctt gttatgacgt tctcattcgg tctgttctcg gcgaacacgg caagagctat
      841 aaacaacaat atcagtgaca gaatctatta tgaaaacggt gctgacgttg tgctgaccga
      901 atatgcgctg tcgacctcgg aggagggcgg atcgagcagt tatgtcgaaa ccgatttctc
      961 tcgttatgag gcgcttgacg gcgtggatat cgctacaaga gtgcttgtaa acgacaacgt
     1021 gaagctgagg atcgcaaaca agaatgttgc ggatcttaca cttatggcga tagagcctga
     1081 caaattcgca cagactgcat ggttcaggaa cgacctgctt ccgatacact ggtggaacta
     1141 ctgcaacgct cttgtcgaga gccgttctgg tgtaataatt tcaagaggac ttgcggaaaa
     1201 agccaatgta tcgcagggtg acgtaatatc gctcaaatgg ggcggaaacg acagccttga
     1261 tacaacggta atggcgatag tagattattg gcccggtctt aaccctaata aatgtgactt
     1321 tgcggtcatg aactacaact atgtaagagc aagaacagag cttgagccgt atcagatatg
     1381 gctcaagatg tcggacggcg ctacaagcga agcactgtac nnnnnnnnnn gtacaagagc
     1441 attgaggacg ctatgctccc gcttgagagc ataaaggaca gctcacagat gctgataagc
     1501 gaaaagaccg atccgtcact ccagggcatg aacggtgcgc tgacgctcgg atttataatc
     1561 ataatggtaa tgactataat cggcttcctt atctactgga tactgtcgat aaagggcaga
     1621 acgctgcaat tcggtatttt aagggctatg ggtgtttctt tcggcgagat aataggaata
     1681 ctgggatatg agcagctgct tgtatcgggt gtttcgataa tattcgcatt tatcataggc
     1741 ggcgttacaa gcgacctgtt tgttccgctg ttccagtata tgtacaatgt aaatgagcag
     1801 attcctccat acagagtagc ggcaatgcag gcagactaca taaagatgta tgtgctgata
     1861 gccgtaatgc ttctcggcgg ctttgcaata ctcggtgcta tcatacgcaa gataaacatc
     1921 aacaaggcac tgaagctcgg cgaggattaa aaaatcgcgg tatgaagcct gtcaggactt
     1981 tgtatgcggg cttatgaagg aggaacattt tggagcttgc tctgggaata ttcaaaaaga
     2041 cacgcttttt gttcgcgctg atcgcggctg ccgcgggtgg tgtaggaatg gcaatgaaga
     2101 atgatacggc ttatgtcgtg tgtataatct tcattgcgct gacgatagcg gcagacgcaa
     2161 cggtgatggt gctcagcaga aagctgaaca taccgataaa gtaccgtgaa aactatttca
     2221 ggttcggcaa gaacaagaag gataaaaagt aatgcagaag atactcggat atatcagaaa
     2281 agccgtacag gagtttgacc tgatagaaaa gggcgataag atagctgtcg ggatatccgg
     2341 cggcaaggac agccttgtgt tgttgtccgg tcttgctctt atgaggcgct ttgccgagtt
     2401 tgactatgat atagtcgctg ttacgatcga cccgggattt aacggcgtgc cgaataacta
     2461 tgaggcggtg gcaaggcttt gtgaaaagct ggacgtgcct ttcagccttg tgcatacgca
     2521 gataggcgag atagtgttcg atgtcagaaa agagcctaat ccgtgctcgc tgtgtgcaaa
     2581 tatgagaaga ggcgcgcttc acgatgcggc aaaggcggca gggtgcaaca agctggcact
     2641 cggtcatcat aatgacgatg ttatcgaaac atttataatg aatttattta aagagggacg
     2701 gataggttgc ttttcgccga aaacacacct tgaccgcaag gatataacgg taatacgtcc
     2761 gcttgtgctt gcgcccgagt cgcaggtgat atccgcctgc aaccgcaaca gccttgaggt
     2821 cgtaaagtcg gtttgtcctg cggataagac tacgacaaga caacagacaa aagagttcct
     2881 tgccgatatg gagcgaaagg ataaaggctt caagctgaga atgttcacgg cgctgagaaa
     2941 gagcggtgtt gacggctggg gctacaagca gaaataaacg agaggtgact gtacttggct
     3001 gttaaaaggc agacgctgaa tatacccgaa caggctttgc aggttcttag agtgcttgaa
     3061 cgagcgggaa acaaggcata ttttgtcgga cagtgtgtag ccgaattgct gaggggcggc
     3121 tcgcctatgg actatgatat aatcacgact gccgactttg aagagatgct gtatgtgttc
     3181 agcgatatga gaatcgtttc gcagaatgag gacatgagca gtgttatggt ttcatcggtc
     3241 ggtcttgtta tacaggtgtc gagctattgc agtgaagtta aggacggcaa ggcggtgtgt
     3301 acggataact atctgtttga ccttgcaagg cgtgactttt cggcaaatgc tatagcatat
     3361 cacccgagaa aaggcttcag agatccgttt gacggtatgc aatgcatcaa ggacggaata
     3421 attacactga aagctgtcgg cgagtatcct gtcgtactgt ggcacgaaaa tgaccttgat
     3481 gaaaatgagc tgactttaaa gctggagccg aagtcaagtc ttgtcacaga cccgttaaat
     3541 atattgacgg cgctcgagct gctcggcgga gagaattatg agatcgacag cgttacggca
     3601 gacgcgataa aggcaaatac ggggcttctg tgtgcaatgc ccggaagaga gctgttcaga
     3661 cgttttacta aggtgcttct tacaaggaat atatctgtcg ttatgacgcg ctttcctgag
     3721 gtgtttgcag cgatcgcgcc ggagcttctg aaagcgcaga ggtataagga cagatatcat
     3781 gactgtacgc tgtgggagca tatttcaaaa tgcgttgagt tcgcacctcc gcaggcagac
     3841 atcagatatg ccgcactgtt ccataacgcg ggacttcctg attgcagatg ccacgactcg
     3901 cacggcaaca cttatttcta cgggcacgcg gagctcggaa ggataatagc ggcgaatttc
     3961 tttgaaaaat acagggcgga gcttaagctt cgcagtgata cggatatgct gatagaggcg
     4021 cacgatacgg tatttaccga ggacagagtg gcgctgaagc gtctgctttg cgactattca
     4081 catgatgagc ttaaaaagct tctcaaactg cggatagcgg acgatacggc aaatcctacg
     4141 gtacatgaag ggcagatcgc cgcatatcgt agggaagtga caatgcttga cgatataatc
     4201 gcatcgggag agtgctacag tgcaactcag cttgccataa aggaaaatga tcttattatg
     4261 cacaggcttg cggcgaacaa ggcgcaggca aaggctgtta taaattcgct gtatgaggcg
     4321 gtgctcgccg agccttcctt gaatgttgct ccggtactgc ttgatatggt gagaaaaagt
     4381 atgcatcgcc gctaaaatct gcaatgtgtg cttacgaaag gattgatgtt atgcagaatt
     4441 gttgtctttg gataggcgga gtgaaggtat ccgaccttga cggcattaaa gacaatttca
     4501 atatgaccga tgttaagggc tattatctcg gcggcagact tgcagactgg ctcagaatgg
     4561 gcggctatga tgccgaggca acaaaaatcg ataacattga caaaaacagc gatatagata
     4621 aggcgcttga agatatattt acaggcaatg atgaagaaac cgacagcaaa aatataagcg
     4681 gggagtacgg cggttatcgc tttcagactg taacgccgcc gtttttaaag cgtgacgcgg
     4741 gcacgatcgt tctgtcggct gacggctctt tcagagtacc tgtaataacc gacagctctt
     4801 ttgcgggatc gtatgccggt tcgtcttttg caggctcttt cggttctttc gactcattca
     4861 tgcacaggca tcagtatgaa tatgagtacg agtacggctc gggtatatca agcttcggct
     4921 cgtttaattt cgggagcttt gacatttcaa gctttatctc gggcagtttt tcgtatgatg
     4981 agctgtgcga gggaggaagc tttacaccgc aggcgctgat gctgttcttt tcgtccgaac
     5041 cgctcaaccg ctatgggtac gggatacatc ttatatgaac ggatttcatt ctttttcatc
     5101 ggcggctttt tttgccaaaa atccgggcaa aaagctcagt atgagccttc ccgacatata
     5161 ttgtacggta atttcggctt tgcaatggcg caaaaacggc aacagtataa ctatgctaac
     5221 cgacagcgca ggagcggagc ttttttcggc tcttgggata aacgatatat ggagtgatat
     5281 aagaattgta atacctgatg accttgacgg gatcgatccg gtaatgttct gggcgggggg
     5341 aatgcttatt gcgctgcagc agttttccgc tccgtgcgct atgatagata cggattttat
     5401 cgtgtgggaa gacccgccgt tcggggataa gataatagcg gcgcatgagg aggagcttat
     5461 gccgtctgtg taccctgatg tatcaacgtt caggctacgc ggcaaggtgc ttgacgaggg
     5521 gcttgattat agtatactgc cgctgaatac ggcgtttttg tatattcctg atgaggattt
     5581 caagcagtat tacacatcac gctcgatagc atttatgaag tcggcagtgt acggcggaga
     5641 ttatcttaca tatatggttt ttgccgaaca gagattgctc ccgatgcttg caaaaagatg
     5701 cggtattgaa tatacgacgc ttcttgacaa ggacaggctt ttcctgccgc aaagcaagta
     5761 tactcatctg tggggcgcaa agcagaaaat gcgtgatgat aaggaacagc ttgaaaagtt
     5821 ctgtgaacgg tgcagagagc ggataagaaa atcgtatccg aggtatgggt atattattga
     5881 taatattgaa aacagtgtga aataaaaggg gttgtgatac aacccggaaa agaaaaagtg
     5941 agtcgaagcg caaagat
//
LOCUS       DAWAOG010000055         5954 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1793_length_5954_cov_2.418715, whole genome shotgun sequence.
ACCESSION   DAWAOG010000055 DAWAOG010000000
VERSION     DAWAOG010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1793_length_5954_cov_2.418715"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    838..847
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4148..4157
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1445 a         1535 c         1262 g         1692 t
ORIGIN      
        1 tttttgatag tcggtgacag tgcagttgca ctgatttact cacgaaatta ttaacacctt
       61 gcaaacacgg aaaatgtttt taaagatgcc ggcactatat ttattgtttt gacgttttta
      121 caatatatta cattgtaatt ttagtaaatt tttggaagca agaaattgtg tcacaacccc
      181 attttgctat ccgcaatata tcatcatcac tcttcgtctg tattttcctc ttcctgcgta
      241 tccttacgct tcatttcaat ctcaagcaga ggtcttaaca gcgaatatct gagcatcttt
      301 ctgtcgttat ccaccatagt ctttacaagc tgttttgtgc ctatgactat tatcatcttc
      361 ttggctctgg taacacctgt gtagagcaga tttctgtaca gcagattttg cgtaaaaccc
      421 gttatcggaa tgatcacggc atcatactca ctgccctgac tcttgtgtat cgtgatagcg
      481 taggcgtgtt caagctttcg tagcatttcg gaggtgtata tcgccattct cccctcaaag
      541 tctatcgtca cattctgaga aaagcggtcg accgcacgga ttattccgat atcgccgttg
      601 aaaatcccac tgcccttttc attgtttttt gtccacagca cgtcatagtc gttctttgtc
      661 tgcattacct tatcgccggt gcggaatatt acatcaaaga acttcagctc tgctttgttc
      721 tgcgatgggg gattaagcgc aagctgaagc tgtttgttaa gctctctcgt tcccgccgct
      781 cccatttttg tcgggcacag tacctgaatg tcgtctatcg gcgaaaatcc gtaggtgnnn
      841 nnnnnnngtg tcgggcaggc gctgttttgt aagctgtatc acaagccccg ctatctcgct
      901 ctcattcggc ttgttcatga agaaaaagtc gttctgtctg tcgtcaagca ccggaaattc
      961 gccctttact atcctgtgtg cattggtgac gataaggctc tgcgccgcct gcctgaatat
     1021 ctctttcagt tcaacggacg ggatatagtg cgacgctata aggtctttca gcacatttcc
     1081 cgcgcctacg gacgggagct gatttgagtc gccgaccata atgaatttag atgtgggctt
     1141 tatcgccctt acaagcgaac acataagaag tgcgtccacc attgacattt catcggcaat
     1201 aacaacatcg gcagggagcg gatttgtttc gtttcgcttg aatttcagct caccctttgc
     1261 ggtataatca acctcaagca gtctgtgtat agtccttgca ggctcaccgg taaggtcgct
     1321 catacgcttt gccgctcttc ctgtcggtgc gcagagcaga atgcgcttct tatgcgcttt
     1381 cagtatcttg ataacgccgt tgagagtagt cgtcttgcct gtaccgggtc cacccgtcag
     1441 aataaacacc gagcctgtaa ggcaggcgtt tatagcggcg cgctgtaagt cctcatactg
     1501 tatattttcg gaaaattcta cccctcttat ctcatcggaa tagtcctttt cgtactgcgc
     1561 cgaggtgcgt atcataaacg caagcttctt agcaatatag gtttccgcaa ggtagtattc
     1621 tggcaggtac acaaactcgc gctttccgag tgtaagcctt gtcacccatt ccttttcctc
     1681 gcagtcgtca aggcaggact caaactgtct gcgttcgatc ccgaggttgt cgcataccga
     1741 ctccctgagc ttttcaacag gcaggcaggt atgacctgcg ttggcgttct cgtgcaagac
     1801 atacactata ccggcgcgca cacggtcgct gttctccgcg tcaaatccga gatctgccgc
     1861 catacggtcg accgaacgga aatctatatc tattccgctc gtacagagta tataggggtt
     1921 tgtttttatc tgctttatgc tgtcgtgctc aaatgccgac catacgctta ttgcgtgtgc
     1981 aggcccgaaa ttatattcac cgaggaactt tatgacctcg tttactcctg ttatcttatg
     2041 atactcattt gatatgtaca gtgccttatc cgaggatatt ccgtttatcg ccgtgagctt
     2101 catacagtca ttatctatta tatcaagtgc ttcagttccg aacgccttta ctatgcgctt
     2161 tgctatagcg ggacccagac cctttataac gcccgagccg aggtatgcca ctatctctgt
     2221 ttcggtctga ggcatagagc gctcacacat tgccgccttg aactgtctgc cgtacttcgg
     2281 acttgtgatc catccgccgc gcagatccag ccgttcgccc tcgttcacat cgccgagtgt
     2341 tccgactacc gggataagtc cctcatcaca gccgagattt ataaccgtat agccgttatc
     2401 ggcgttttta tatataacat cctccacaga gccgctgagc tctacggaaa tatcgacctt
     2461 ctgattatcc aattacattc cctcgttttt cttcttagcg ttacttcata cataccctcg
     2521 gacaatcggc agaaagcgca cggcatatag tgataaattc cgtgtcggct gtcaggcttt
     2581 ccggcactat tatcacaaca tcgccgtttg tcgccttaag cgcgctcctg acctttccct
     2641 caacgtcatc gcacccatcg ggtctgacat atatcggcgg cgcattggca gtacccagac
     2701 tgccgatgaa cagcacaacg ttcacagcgc ccataattcc gagaaaaatc ataccgaaaa
     2761 gcattatgta tgcaccactc ataagatttc actcctttta tagtttatct cattatatgg
     2821 tgcagacgtt tattctgtta cggattgcag gtacagagaa ttgcgttcca gccctctttt
     2881 tcagatacgc tttctaccct gatattattt tccttaagtg cggacagaac ctcgtcaagt
     2941 ctttctgtta tgatacccga tactataagc ttgccgttct tctttaaaaa tccgctgaaa
     3001 tacggcgaca ttgatataat tacatctgcc acgatattcg ccgtgataat gtcaaagcct
     3061 gtgcctatct tttctctgag cggttcgtca tcactgatat ttccgacata agccgacacc
     3121 atatcactgc cgaaaccgtt cttttcggta ttttcctttg cggtgcggac ggaattttca
     3181 aatatatcta ccattaccgc ctgctttgca ccgagcagaa gtcctgcgat ggacagtata
     3241 ccgcttcccg tgccgagatc gagcattctg tcgccctcat ttatctgctt ttcgagcagc
     3301 tccattacaa ggcgggtcgt gtgatgctga cctgtaccga atgacgaagc ggggtcgatc
     3361 tcaagtatct ttctgccgtc tgtgttctct acttcttccc agcttggctt tactatcagc
     3421 ttttcaccga ctgtaaaagg cttatagtac tgcttccagt tattagccca gtcctcttca
     3481 cgcacacagt ctatctccac atcaagacta ccgtaaaaat cgtccttatc gttagctttg
     3541 agctcaccaa gtgttccgct gagagccgac agcatttcag caccctgctc attatcctga
     3601 acatagcagg taatatgcgg ctttacggtt ttcagtccca taaggctgtc atctacatag
     3661 tcccagttgt actccttgtt ttcaaggaac tcctcaaagt ctgcggggtc gtggattata
     3721 aacccgttaa gtccaagctc cgataggcgc ataataagca cctgcaccgc ttcacttttc
     3781 gtatatatgt ctacctgtct gaactgcata gtaatacctc ttgttttctt atttgcccgc
     3841 cggcataaat atctattata tattatccta catttcgggc aaaaaagcaa gtgtttacaa
     3901 caaaaatgca ccgcccttat caaaagagcg gtgcatcaca ttcaaggaag aaccaatgct
     3961 aatttctgat ttttatcaat cgttgtcctg taaagcctca taagcctctt cgcctgtacg
     4021 aaccttgata acattctcaa cgtcgtatac gaatatctta ccgtcgccta tcttaccggt
     4081 gtaaagtgca ttctttgcaa cttcaacaac cttttcaaca ggaaccttgc atactacgat
     4141 ctcgaccnnn nnnnnnngtc ttgcgtctac aggaacgcca cggtagaact cagttgagcc
     4201 cttctgcata ccgcagccca ttacatgaga taccgtcata ccggtgatac cgatagatga
     4261 aagcgcgtgc ttgagaacct cgaacttctc ctgatttgcg atgatatcta cctttgtcat
     4321 ctttacatcg cttgcgctga taggagcttt tgcctttgaa acgtgctgta caggtacaga
     4381 tgtttcaaca ggagcagcct cagtaacaac tgcagtatct tctgcggaag aaccgctgta
     4441 tacgctgtcg cccgcgataa cgaagtctgc atatgagctg ggcagaccgt gttctgtcgt
     4501 atcaagaccc ttgagctctt cgtctcttga agctctcaga ccgatcgtgt gctttaagat
     4561 gtagaatgtc aggaacatcg ttactgctgt gaatgcgcaa acagcaacta cgccgagcgc
     4621 ctgtacgccg agctggctga aaccgccgcc gtagaacaga cccgtgatac cgttctgacc
     4681 cttacctgtt gcgaataagc ctactgcaag tgagccccat acgccgttga agaagtgtac
     4741 cgctacagca ccgacagggt catcgacttt gagcttgtaa tcgaggagcc aaacgccgaa
     4801 gcaaaccagg aagcctgaaa caataccaat tatcgttgcg ccgagtgcat ctgtatctgc
     4861 acagggagct gtaatagcta caagtcctgc gagtgaagcg ttaagacaca tcgaaacatc
     4921 gggcttgccg tttttgagcc atgtgaatat catcgttact accgtagcaa ctgcaggagc
     4981 aattgttgtg gtaaggaata tatttgcgag gtagtttaca tctgatgcgg cagcaccgtt
     5041 aaatccgtac caaccgaacc agagaatgaa tacgccgagc gcgccgaggg ggatagagtg
     5101 gcctaagata gctctgggct tgccgtcctt gctgaactta ccgatacgcg gtccgaccat
     5161 tgcggcaccg ataagagctg taagaccacc gaccatatga attgccgttg aacctgcgaa
     5221 gtcaatgaag ccaagctgtg cgagccagcc gccgccccag atccagtgag cctcgatagg
     5281 ataaacaaca aggcttataa tgaatgagta gatgcagtag ctgatgaact ttgtacgctc
     5341 tgccatagca cctgaaacta tcgttgcggc agtagcacag aatactaagt tgaatacgaa
     5401 ctctgcacat ctgtcaaagc tggaaagctg agtcagaacg tcaaggttcg gaacaccgat
     5461 aagtccgaag aatgtatctt caccgagcat cagaccgaaa ccgagaagta cgaatgcgat
     5521 cgtaccgagg cagaagtcca ttaagttttt cattataatg ttgccggcat tctttgctct
     5581 ggtgaaacct gtttcaacca ttgcgaaacc gcactgcata aagaatacca atgcggcacc
     5641 gataaggtac catataccgt tgccgtcgcc gacattgaac attgtggcgt taacggtttc
     5701 ctgaattttg tctgccgatt cggcagctag agttagcgtg gataagagat ccattgataa
     5761 ccgccccttt caaaaggtaa ttttctgact gcggctgtgc cgctttttcg ttaagtgagt
     5821 ctgaaaaaat cctatctgca cccctctgcg cctttgcaag atcttgcaga acttttgcaa
     5881 tctcactcaa aacaaaagga gccgcacgaa cttttcggtt cgtgtagctc cgttgcttac
     5941 aagtattatt atac
//
LOCUS       DAWAOG010000056         5928 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1800_length_5928_cov_2.507577, whole genome shotgun sequence.
ACCESSION   DAWAOG010000056 DAWAOG010000000
VERSION     DAWAOG010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1800_length_5928_cov_2.507577"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1982..1991
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4395..4404
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5210..5219
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1388 a         1476 c         1407 g         1627 t
ORIGIN      
        1 ccgatgatta ttatgattat aacggctttg ccgaaaacgg tgtattattt gccgtattct
       61 ctggcagcag agattgggcg gtaagcacta aaggctcggc aataaaaaca ttcggagatt
      121 atcgggacag catagtcggt acgataaaaa gtgagctgtc cgatggtaat tattttgatg
      181 cgtttgcggg atatgcggat aagtgcgatg agatgctggc ttacgaaagc gagcacggaa
      241 gtgcttatgt agcctctcag caaaaggatg cgtctgatta catcatagct gttgttatca
      301 gcgttgtagt gggaattatt ataggcttta tcggttccgg cataatgaag tccaagctga
      361 agagcgtgaa ctacaagtcg ggcgcaacag attatattgt tcctgaaagc ttcaaactcg
      421 ataattcccg tgatgtatat ctttataaca cgataacaag aacagagcgt gaaagcgaat
      481 caagctcatc atcggacggc ggttctcata cgagctcctc gggtagttcg cacggcggcg
      541 gttcgggtaa attctgataa aatacaaatg aaaaggaata cgggtatcgg cagtgccgat
      601 acccgtgtat ttttgatcgg tatctatttt cctacgcaaa aacgcgagaa tattctgtct
      661 attacctcct cgcttgcggt cttgccgctg agctcgtaca gactgctcag tgccgcttcg
      721 agctcaagtc ccgcaacgtc tgcggtcata ccgccgtaca gcgcgttata ggctctgttg
      781 actgccgcct gcgccgataa ggcacactgc cgctggcgct cgtttgcgag gaagcccgcc
      841 gccgggtcga gcttacttat accgcaacgt tgctttattg ccagttcaag catttcagca
      901 ccgtcacctg tctttgcgct gattattact gccttgccga gcctatcttc aatatatgcc
      961 gtgtccgctc ttgtttcaag gtcggacttg tttattatcg gaatgactgt cttgtccgaa
     1021 agcagcccta taagtcgctt gtcatcatcg gacagctctc tcgagccgtc aaataccgca
     1081 aggataagct gtgctgtgcc tatgcggtct atcatacgct gtacgccgat gttctctacc
     1141 gtatcgtctg tttctcttat gcctgcacag tcggctaact tcagcattat atcgccgagc
     1201 tgtacggtgt cctcgacaat atctcgggta gtaccttcaa tatcggtaac tatgctcttc
     1261 tgtacgcctg tcagcaggtt cataagcgtt gactttcctg cattgggctt tccgactatc
     1321 gcggcggcta ttccgtcacg cagtatcgcg ccgcagtcat aggtcgctat aaggctgtcg
     1381 atattttcct ttacggcggt cacgcttttc agccagtccg cctttgctat ttcgtcctcg
     1441 tcctcgctct catcgggata atctatccat acggctatct ccgaggtaat tgcaattatc
     1501 tgttcgcaaa gtgtttctat cctgcggtag agtgcgccgt cagtctgtgc ccttgcacag
     1561 gataacagct gatcgcttcc ggcgtttata aggtcgataa caccctctgc ttccgtgagc
     1621 gagagcttgc cgtttacaag cgcctgcttt gtaaattcgc ccgcctgagc cattcttgct
     1681 cctgccgcca gcaccgcttg cagaactctc cttgtaacgt gtatgccgcc gtggcaggtt
     1741 atctcggctg tgttttcgcc ggtatagctg tgcggtgcac ggaatattaa aagcacacca
     1801 tcgtcgagct tcgcgccgtc cgccgcgata tcaccgtatg cggcggtata gccgtccatc
     1861 ttttctatat cgtgcgcggg gtatttcggt gtgaatacct ttgccgcgac tgtaagcgcg
     1921 tccgtacccg atattcttat catcgaaacg ccgccgcttg ccataggcgt tgatattgcc
     1981 gnnnnnnnnn ngctatgcag tctgccatta tatcttaatc cttcttaccg aataattttg
     2041 caaaaaagcc tttcttcgcg ggcttttcgt ctgtctgttc ttccgtgctg ttttcctgct
     2101 tgtcatcgcc gttatcgtaa tagctctctt cttcctcctc ggaaagcgcc gtttcgataa
     2161 agctcttctt gagcagctcc tcgccgcttt cgttaagcgt atatgtccac agctctgtcg
     2221 gagtgtttat aacaaaatcg tctatattat caaggctgag aagcgtctga agccgctcgc
     2281 ccatagtatc tatcacctgc tcctccatac ccgagaaaaa ttcctcgcag gttatgttat
     2341 actcatcggc ggggttcttt ccgcctattc ttgtaaggtg gataccgtca cgcagatagg
     2401 aggtgtaatc gagataatcg ctccagtatt cgtttatcac ctgtagtatg acacgctttt
     2461 gcagagcatt gaccttgtcc tcgccgaact tttgtgcggc ggtgctgtaa tcatcagcaa
     2521 attcgctctg ccagatatcg actgtcgact cgcctgtcag gaatgctctt cttctgccga
     2581 ataccgcgtc acggtgcttt tcgcctatca tagtgaactt gagcagacgc ttacgcagct
     2641 cgagcgtatc gccctgtgct atacgctgaa tgcggtcgac ctcgcgcagt acgatcttat
     2701 cgtcaatcgg tctgcccgtt tcagcagtgg ggtagtgcct tttcggaata agcttcttta
     2761 tctcgtactt tgtcattatc tcgtcttcaa gtgagattat aaaacggctc tcgcctatgt
     2821 caccctgtct gccgcttctg ccgcgcagct gcatatcgcc gcgttcactc tcgctcataa
     2881 atgtgccgag tatcagcagt ccgcctgccg ctacagcctc gtccttctgc gattcgtcag
     2941 cgccgcccag ctttatgtca acgcctctgc ccgacatatt ggcagatatg gtgacagcgc
     3001 cgggtctgcc tgcttctttt attatctcgg cttcaagctc atcgttcttt gcgttgagta
     3061 cgtttgcggt gatgccctct gccgcaaggc gttcattcag ctcttcactc agctctatgc
     3121 ttgacgttcc gacaagcacg ggctgatttt ttgcgtgggc ggtttttata cattcgacaa
     3181 tagcgttatc cttagccgct tttgtgagat atacttcata agggtgatca acacgtctgc
     3241 aaggagtatg agttggtatg accgttacct taagatcgta taactgccag aactcgtcct
     3301 ccgaactttt cgcagtaccg gtcataccgc tgagaagcgg ataacggcgc agatagaact
     3361 gcataggcac aacgcccata ataacaccgc gacttgtgca ggtaatgcct tctttcagct
     3421 cgactgcagg ctgtaacgag ccgggatagc ggcggttctc agccgctctt ccggtaaact
     3481 cgtcaattat tcttatatta ccgtccttga cgatataatc aacgtccttt tttagcaaga
     3541 agcacgcttt aaggcaatct gtaatgcgta tcagaagctc gttgttctcc tcgtcataaa
     3601 gccccgagcc gtccgttata agctcctcac acttatcctc gcccttttcg gtaaggtaga
     3661 tagtgccaag ctcttcgttt atctcataca tactgctgtc aaaatccttg acaaactcgt
     3721 atacctcggg gagcttttca tccggcttta cggcggtttc acccgctata acaagcggta
     3781 ctcttgcctc gtctatcatc atgctgtcag cttcatcggc tatgcagaag tcgaaatccg
     3841 tctgcacgca gtcctcgggc gttttcgcaa cgaagtcacg caaaaaatca aagcctgcct
     3901 ctcttgcggt tatatatact atctgatttt tatacatctc ttttttatcc gcaatatctg
     3961 tcgaatgcag gatacaagcc aagcttatgc cgagtgcgtc atatatcggc ttcatccatt
     4021 cgctgtcacg ctttgcgaga taatcgttga aggtgagtat tctgacattg tgaccgtttt
     4081 tcgccatata gcttgcggca aatgcggcac acagcgtctt gccctcgccg gttttcatct
     4141 cggttattct gcctttggta agctctgcgg cggcggtaag ctgttcgtca taagggataa
     4201 gtccgaggta ttccttgctt gcgatagcgc aggcggcgaa tatctcgttc atatccgtac
     4261 tgtctttaag ctcgtctatt gtgccgacct cggcaagtga gcgtattttt tcgatggata
     4321 tataagtttc agccattttt gctgtccttt cagaataata atgtataaat aattatacaa
     4381 aacggggttt ggggnnnnnn nnnnggtttg gggcgtagcc ccaagcgggg tgagggcggc
     4441 agccccacac tcagcccggc acggacgctg ggcgtgtatg cccaaatgct ctgcacgatg
     4501 cagagccaac taagcataca cagtccttca ccacggggaa ggggtttggg gttagggatt
     4561 aagaagttgc tctttttgta aaaaataaac tttcttataa tcttaaacgg catttcaaag
     4621 ccgtctatat cgtttatttt accacagtga aatgaaaaaa gcaattattt ttaaaaagca
     4681 cttgaaaaat acggataaat gtgcgacaat aatatttata tagcggcgtt ctgccgcaaa
     4741 aaccgataaa ggaatgactt actttgaata atgcaaaata cgctgtaata cttgccgcag
     4801 gcgatggcaa gagaatgaaa tccgataagc cgaaggtgct tgccgaggtg ctgtttcagc
     4861 ccatgctcgg ctgggtgctt gacgctgtaa agagcgcagg cattgacaat accgcagtaa
     4921 ttgtcggcgc tcacaaggaa caggtaacct cttatcttga aacgcttggc gaatacccca
     4981 catttgagca gaaagaaagg ctcggcacag gtcacgcagt aatgcaggca aaacccttta
     5041 ttgaaaaggc acaggctgaa aatgcggata tacttgtagc ttgcggcgac gctccgttca
     5101 ttgacgcaga caccataaca ggctcttatg agtatcacaa gaacagcggc aacgatgtta
     5161 cggtagtatc cgcaattgta gatgacacca caggcaaagg cagaataatn nnnnnnnnnt
     5221 tttagataag ctcggcaaca acaatgccgc aggcgaatat tatcttaccg atactgttgc
     5281 cgttgccgaa aagaagggcg tatacacggc agacagcgca gacgttgtac tcggcgcaaa
     5341 cagccgcagt cagcttgccg ctttgaatga agtcgcaaga aagagaaagc tcgctgagct
     5401 tatggacgag ggtgtggata ttcctctgtc ggacgggatc ataatcggca aggacgttaa
     5461 gatcggtcac gatacagtta tacttccgaa tacgataatc aaaggaaata ctgttatcgg
     5521 taacggctgt gagataggtc ctaacagcta tatagataac tgcgttatag aggataacgt
     5581 tatcctcaac aacgtacagg cacacgaaag caaggttgac agcggagcaa aagcaggacc
     5641 ttttgtacat ctgcgcccga atacacatct gcatagcggc gtaaagatag gcgactttgt
     5701 agaggtgaag aactcggagg tcggcataaa cacctgtata gcgcacctga catatgtcgg
     5761 cgacagcgat gtgggcaagg gtgtaaactt cggctgtggc tgtgtaacgg caaactatga
     5821 cggtatcaag aagtaccgca ctacgatagg cgacaacgct tttatcggct gtaacacaaa
     5881 tatgatagcg cccgttacta tcggggataa tgcgactact gcggcagg
//
LOCUS       DAWAOG010000057         5878 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1819_length_5878_cov_2.336253, whole genome shotgun sequence.
ACCESSION   DAWAOG010000057 DAWAOG010000000
VERSION     DAWAOG010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1819_length_5878_cov_2.336253"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5224..5233
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1605 a         1198 c         1553 g         1512 t
ORIGIN      
        1 gaatatcaca tatgagagaa atccgggcgc tgctttcagc atacttgaag gcaagcagat
       61 gtttcttgtg atattcaccg cgataataat tgtcgctatg ctttatctga tgatatcaag
      121 aagggtcaag cgccctacct atatatggtc tatgtcgctc attgttgcgg ggggactcgg
      181 aaaccttatc gacagattgc tgagaggcga ggtagttgac tttattgatg taaggataat
      241 caactttgcc gtgttcaatg tggcggatat atgcgccgta ctcggtgcga taggattgct
      301 cttattcgtt gtaattgatg agataaagga acagaaaaac aagagatcgg caaagaagaa
      361 tgagaatgcc gatgataaca aggaacagga cactgccgtg acaaaagcag aaacggtaag
      421 tgaggaaaaa taacaccgct acggagggta atattgcatt acgaatttac ctgtgaaaca
      481 ggcggcgaaa gaacagacaa atttcttgca cagcaggata caggaataac ccgatcagca
      541 gccgccgctc ttattgagca gggctgctgt cttgtaaacg ggaaaaatgc cgccaagaac
      601 cttaagctga aagcgggaga tgttataacg cttgatgtgc ccgaggcaca gcctgccgac
      661 attctgccgg aaaacatacc gcttgacatt gtctatgagg ataatgatct gcttgtagtc
      721 aacaagccca agggtatggt ggtgcaccct gcgccggggc actacagcgg aacgcttgtc
      781 aacgcgctta tgtatcactg cggcgacagc ctttccggga taaacggggt catccgcccc
      841 gggatagtgc acaggataga cagggataca agcggtctgc tgatcgttgc caagaatgac
      901 acggcgcata atgtgcttgc cgcgcagata aaggtgcaca gcttcacaag agaatatatg
      961 gcagtggtgc agggcgttat caaagaggac ggtacggtca acgccccgat aggcaggcat
     1021 aagacagacc gcaagaagat gtgcgtcacg accgagaact cgcgggaagc ggtaacgcat
     1081 tactttgttg agcagactta caggaacaat acgcttgtaa gactgcggct tgaaaccgga
     1141 agaacgcatc agataagggt ccatatggcg tatataggtc acccggtgac aggtgatgaa
     1201 gtatacggaa acggcagtcc gaagtggctg tgcggtcagt gccttcacgc aaagaagata
     1261 ggatttgtcc acccgaacgg acagtatatg gagtttgaca gtgagcttcc cgactatttt
     1321 cagaagctgc tgaagaacct tgaaacacag aagacagaga tataaaacgg cataaacaaa
     1381 ggaaaagaag actatggatt tctcaaatgt aattataatg agcgaccttg acggtacact
     1441 gcttaatgat aagaaagaga tctcaccgcg tgatatggag tcgataaatg acttttgcgg
     1501 caagggcgga atgttcacga tagctaccgg cagaggctat tctatggcaa agccgatagc
     1561 ggaaaagctt aagctgagga tacctgcggt catcttcaac ggctcggcgg tgtacgattt
     1621 tgacaaggaa gagttcctgt ggcagtgcga ggtcacgcag aaggcaaggg attacataag
     1681 gcttgttatg gagcggttcc ccgatgtggg gatagaggtg ctgcacaagc acacggtata
     1741 cgtccctgcg ctgaacgatg tcgagagggc gcatatggcg cttgagaatg tgcacggcga
     1801 tgtatgcagt gttgacgata tcccgcatga gggctggctt aaggtgctgt tcgcgtaccc
     1861 ggaggacaag atgccggagc ttgcgaggtt catagacgag cactgcgcag aggggacgaa
     1921 cagggtgctt tccgcgccgt tcttctacga gctgctgccc gagggagtga gcaaggcata
     1981 cggatacaag cagttactga gggtgacagg cagcgagggc aggttcactg ttgcggcggg
     2041 cgactacccg aatgacgctg aaatggttcg cagtgcggat ctgggcgttg ccgtaagcaa
     2101 tgcgcatgac aatgtaaagg cggcggctga tatcataata ggctcaaata atgatgaccc
     2161 gatgacgcat attattgaga aaatcaaaca aatgtaatat ccggtacgaa ccggtggttc
     2221 attttggtac agttttggta tgacagataa aaaacaggtc gcaaatatgc gggttatccg
     2281 aaaagcacgc cggtatccaa ttactgtaaa caaagtcgat gcgaataagc accgtaaaat
     2341 ttgcagtgaa attatgcttt gatattttat gatatgcgga taatatcata gtgtgcgata
     2401 tttattgata aacgtaatga ttatataatt taaattcaga aaggaaaatt tatgaaacga
     2461 gagcctgatt acaagctcaa aaaacgcatg gaacgaattg agcatgaaaa gaagatgaac
     2521 aggacataca accgtaactg gatcatatgt atcgggtcgc ttgcgatcat tatattggcg
     2581 gtgtttgtgt tcggcggaaa gtaatatcgt gtaatacttg acgataagcg gtataggtga
     2641 tataatgata ttattataag cataatgaaa taatcttgaa atggtgagag atatgtgtgg
     2701 gatcgaaaaa tataaattgt gcacgttaag acttgtgaaa tcctatcggg tgaagtctgc
     2761 acaatatctc tgcgtaaaat tcaatattgt aattgaggca cagttcatgg ggtgaactgt
     2821 gcctttttat attatttaaa agaaagaggg tatattatga gatgaaggca tctttcccga
     2881 acctgtaaca ggtgaaacgg aaaacaacaa tgttgtcact gcaacggcag atgacagcgg
     2941 tcttgtctac ggtgacaatg ggactctgac aaaggcagag tggcttcatg acctagtaac
     3001 ggtgtttgac atgaaggttg aagagggtga cccgcccgac aactatttca ccgatgtggc
     3061 agatgacagc gaatattatg atgatataat gcttgctact gagttcggtg ttgtaaatgt
     3121 cgaagcaggc ggcgagctcg gacctgacga tacggttgac agagaatatg cgtctgtaac
     3181 gctcaacttc tgtcttggct ttcagcttga cgatgataca tatactttca gcgaaagcag
     3241 tgatgaagca tatgcagatg agctgacggc ttttcaggtt gcgttaaaca gaaactggtt
     3301 tgcgctttcc ggcggcgctt ttcttccgtc aaaagcgatt acctcagccg aagccaaagc
     3361 tatgctctca gacgcacagc aggtactggc tgacgctcag atcgacgata caaaggaagg
     3421 cacatttgag tttgcagact atgtaattga ctttacgccc tatcaggtgg ttgagttcaa
     3481 tgaagacaat actgtaacta tatatgcgcc cgacaaaaaa cttacagtcg gcgatacgtt
     3541 tgttgtatat gatcaggagc tcccttttgt atacactgca acggctatta aaacagacgg
     3601 cagtgacatt atagtaacaa ctacagatgc gcctgatgac gctattgtga actatgttgc
     3661 cgaaggtacg ataaaggcgg atatagctac ctttaatccg gctgacaatg tagatgtcac
     3721 ctatgtaaag gacgatgtaa cagctcttac catgagtaag gcgaggggcg ttacggttac
     3781 aaaagactca agcaatgcca caacgaccta tagagcaagc ggtcttaagg cttcgactgt
     3841 gtacaagttc agagtaagag cgtataagat gagtggtaag actgcggttt acagcgcgta
     3901 tagcactgag ttagccgcaa ggactaatcc gtctgttatg acaggtgtga agctcagcgg
     3961 cagagcggag taagggcata taagatgagc ggtagcacgg cgctttacgg caactacagc
     4021 gctactgtta cggcaaggac aaatccttcg attgttaagg gcgttaagat tggcggcaag
     4081 gctaaggacg ctttaagagt gaactggact aagaacgctt ccgcgcaggg ttacatcgtg
     4141 gagatgtaca agggcggaaa gtgggtgcgc gttgctaaga tcactaatag caatacgact
     4201 actttcagaa aggcaggact tgctaagaat actacttata agttcagagt aaaggcttac
     4261 tatatgagcg gaaagacggc gctttacggt aattacggta gtgtgagcgg gaagacagcg
     4321 gcaaaataac gctctgatta aaagcaaaaa tcaataatta cgaccgggta gcgatgggaa
     4381 aatcgttgcc cggttttttt ggtggggata ttgcggggtt ggcgatgttt gctggagtgg
     4441 acgcaatcgg tgcggcggga ggggcgatcc ctcagtcttg acggcagaaa aaatccactg
     4501 cgttgaagca gtggatctat tactgttgaa ttgatattgt gccgccgggc aattttacgt
     4561 ttgcttactt ggactcttca gcgtcgtgta ctgttctgtt ctcggcgatg ctgctcttta
     4621 agatacggat ctttgttctg tctgagcctg tttcgataag aactgtatca tctgtaactc
     4681 ttaatactct gccgataata ccgcctgttg ttactacctc gtctgctacc tcaaggttgt
     4741 tgagcatctc ctgcatcttc ttgccgcgct tcttctcggg tctgattatg aggaagtaga
     4801 atataactac catcagtgca agggggataa gcattgtgag aatgctgagg atatcgtttc
     4861 cgccctgtcc tgtggttgtt gccggctgtc cgcttgttaa taatgtcagt aaatctgtca
     4921 ttttgtgttc cctttctttt tcgtgcgaaa ttcgctattt atcttattat aacttattaa
     4981 acccacaatt gcaagcactt ttgtgtattt attcaaatct tatgtcggta aatccgagct
     5041 tttcgagcgc cgttttacaa tttttgatat catcggcgga gagagttgag ctgccggtaa
     5101 tgccgaccga gataccggta tcgcctgtag tcttctttac aagcgtgaat atctgcgtta
     5161 ctgcctttgt gatatccttt tcgactgttg tagtcttgtc ggctgatgtc tgaagctttg
     5221 tacnnnnnnn nnnccgagaa gcgatgtatc cattgttacg agcactcttg cgtctgccat
     5281 cttgtctttt gacgaccata tttcggctgt gccgtcataa gctgttgccg acattgtaag
     5341 cagtgcgtct gcctttatct caacggtcgc ggttgccttt gacgctgtag ctttctttgc
     5401 acacgcctcg gcaagtgccg caagcttttc gcttctgctt gcgttctgta attcctttga
     5461 caggagcgaa tagtcatatg actggaagtt cgggaagata gtattttcaa gctcgatatc
     5521 gacaaatcct gcctttgcaa gctcgccgca gatatcggca taataattta ccgcatcagc
     5581 cgagtagggc gttgtccacg gcttgccgcc gttgtctgcc gccgcatcaa gccagctcca
     5641 gttgccgtct gtgattatat atcccgcact gtcgaaaaga tacggagttt tgcggtcata
     5701 gagcgttgtt atagccgccg cgggggtcat gcctgccgcc ttgcaagcct ttactatctg
     5761 tgccgccgtc agcttgccct tgttcacgtc agtgttgtcc ttgaccgctt taagcgatga
     5821 tttatataac agctcgcctg tcgtgttttt gagcgtaaat acgactgtgt tacagccg
//
LOCUS       DAWAOG010000058         5740 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1869_length_5740_cov_1.952507, whole genome shotgun sequence.
ACCESSION   DAWAOG010000058 DAWAOG010000000
VERSION     DAWAOG010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1869_length_5740_cov_1.952507"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    472..481
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1815..1824
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2411..2420
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3559..3568
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5220..5229
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1830 a         1071 c         1432 g         1357 t
ORIGIN      
        1 atccagacta taggcagagc ggcaagaaac gcagagggca aggtcataat gtatgccgat
       61 acggttaccg acagtatgga aaaggcagtg accgaaacca accgccgccg tgagatccag
      121 atgaagtata acgaagaaca cggcattact cctaaaacta tcgtgaagga cgtaagggcg
      181 gtgctggaaa tatccacagg caaggataag gacaagaagc gaaagtacac aaaggcggag
      241 cgtgaggcgc ttatcaagca gtataccgct gagatgaaga atgcggcaaa gctgcttgac
      301 tttgagcacg ccgcatatct gcgtgataag ataaacgaac tgaaaggtca atgatatgga
      361 agaattaaag gatatccccc gacagcaggg cgggcaatac ggcgcaaggt acaaaaagcg
      421 ccgcagaagg cccgatataa aggcaatagc catatttatc ctgctgataa cnnnnnnnnn
      481 natctgcgtg ataagataaa ggagcttcag gaaagcaagt aggcaattaa cggagatgac
      541 aaaagaaaat gggaatagat aaaataatca tcaagggcgc aagagagcat aatctgaaga
      601 atattgatat agaactgccc cgtgacaagc tgatagttat gacgggactt tcgggatcgg
      661 gaaagtcatc gcttgcattt gatactattt atgcggacgg tcagagaaga tacattgaga
      721 gcctgtcatc atatgccaga atgttcctcg gacagatgga aaagcccgat gtcgacagca
      781 tagagggtct ttctcccgca atatcgatag accagaagac gacctcaaaa aacccccgtt
      841 caacggtcgg cacggtaaca gagatatacg actatctcag actgctttat gcaagagtgg
      901 gcatacctca ctgtcccgtg tgcggtaagg aaataaaaca gcagacggta gatcagatag
      961 tcgataaagt tatggaactg cccgaacgca caaagtttca gatcctatct ccgatagtaa
     1021 gaggcagaaa gggtgaatat cgcaaggagc ttgaggagct tgtaaagcag ggctatgcaa
     1081 gagtgcgtgt tgacggcata atctatgacc tttccgagca gataaagctc gataagaaca
     1141 aaaagcataa catagaggtc gttgtagacc gacttgtaat gaaagaggat atcaagtcgc
     1201 gtttatccga cagtatagag gtcgcgggaa atctctccgg cggacttatc cttatagatg
     1261 taattgacgg tgaggaactc agcttttcgc agaactatgc ctgccccgaa cacggagtaa
     1321 gtatggagga gcttgtgccg agaatgttct cgttcaacaa tcctttcggt gcgtgtccta
     1381 cctgcacggg tcttggcgtg ttcaagagaa ttgacattga ccttatactg cctaaccgcg
     1441 accttactat ccgcgaaaat gctataaagg caagcggctg gaagtatgcg gacggaacta
     1501 tagcgcagat gtattttgac gctgttgcaa acgaatacgg tttcagcgtg gatcagcccg
     1561 ttaaggagct gacaaaagag cagttagacg ctgtgcttta cggcacaggc gaaaagaaga
     1621 taaaggtcgt gcgcagtaaa gagcagggcg gcggagtata tatggcggca tttgagggcg
     1681 tagtaaacaa ccttgaaaga cgttataagg aaaccacaag catatattca agagaggata
     1741 tagctcagta tatgggcgat gccgtatgcc ccgactgtca cggtcagcga ctgaaaaagg
     1801 aagcgttgtg cgttnnnnnn nnnngcgtta ccgtttgcgg taagaatatc aatgaatttt
     1861 gtgatatgag cataaccgaa gcgcttgaat ttgtcaacag cataacgctc tccgaacgtg
     1921 atatgatgat agcaagtcag atactgaaag aaataaagga gcgcttaggc ttccttaaca
     1981 gtgtcggact tgaatatctc accttatcaa gaacagcagg tacgctttcg ggcggtgaaa
     2041 gtcagcgtat aaggcttgca acccagatag gaagcagtct tatgggtgtg ttgtatatcc
     2101 ttgacgagcc gagcataggt cttcatcaga gggataacga caagcttatc gcaacgctga
     2161 aaagactgcg tgaccttggc aacacgctta tagttgttga gcacgacgag gatactatga
     2221 gggcggcgga ttttatcgtt gacataggtc ccggtgcagg tgttcacggc ggtgaggtta
     2281 tctgtgcagg tacgcttgac gatataatga agtgcgagcg ctcaataaca gggcagtatc
     2341 tcacgggaaa attaaagata gaaatacccg aaaaacgcag aaagcccact aagaaatggc
     2401 ttaagataac nnnnnnnnnn gacgcgatgc tcaacggaca cggcctttat atgggtgtgt
     2461 ccggcagcgg caagagctcg cttgtaaacg agataatcta caagcacctt gtcgcaaagc
     2521 tgaaccgcgc gagaataaaa ccaggtgcgt tcgatacgtt cgagggtacg gagtacctcg
     2581 acaaggtaat agcgatagac cagtcgccga taggcagaac accacgaagc aaccccgcaa
     2641 cctatacggg tgtattcaac gatataaggg agcttttcgc atcgacaaat gatgccaaga
     2701 tgaggggcta tacgagcggc agattttcat tcaatgtaaa gggcggacgc tgtgaagcct
     2761 gcgagggtga cggtatcaac aagatagaaa tgcacttcct gcccgatatc tatgtaccgt
     2821 gtgaggtctg caagggtaag cgttacaacc gcgaaacgct cgaggttcac tacagaggta
     2881 aaaatattta cgatgtgctt gaaatgacgg tagaagaggg catagccttc tttgaaaacc
     2941 acgataagat aaagcgcaag ctacagactc tttacgatgt aggtcttggc tatgtaaaga
     3001 taggtcagcc cgcaacgacc ctttcgggcg gcgaggcgca gagagtaaag cttgcctccg
     3061 agctttcaaa gcgcgcaaca ggcaagactg tatatatcct tgacgagcct acgaccggac
     3121 ttcacaccgc agacgtgcac aagctgatag acgtgcttca gaagctcaca gacggcggaa
     3181 acacggtact cgttattgag cataacctcg atgtgattaa aacagccgac tatatcattg
     3241 atatgggacc tgagggcgga agcggcggcg gtacggtgat cgcgaccgga acgcccgaga
     3301 atgtagcggc aaaccctgta agctacacgg gcaggtattt agcacctatg ctcggagttg
     3361 aaagaatcga acaatcaact cttgaataaa agctgatttt cattagtgat atattcacgg
     3421 gaaacacttg acaacgatta actgtgatga tatactaact ataaagctaa gagatattgc
     3481 tgatgaaatg aaaaggtgag agatatgtgc aagacattaa ataccaattt atgtaaacgg
     3541 ggggggggct gagtaacann nnnnnnnnct tgtgaccttt gcggcgatgg ttgtgctgtg
     3601 cgcggtgtgc acgggggcgg agacttatag ggactatgag ttcagcgtac tcagtgacgg
     3661 tacggtggag attaccgatt ataacggcag tgcagaaaag gttgatattc cgaagacgat
     3721 tgctgggaag agcgtgacaa gtataggcga gtatgctttc tgcaattgca cgagctttac
     3781 aagtataaca ataccggaca gcgtgacgag tataggcaac gatgctttca acggttgcac
     3841 aagccttaca agtataacga taccaagcag tgtgacaagt atacgcacaa ggactttcaa
     3901 aggttgcgca aaccttacaa gtataacaat accggatagt gtgacgagta taggctggtc
     3961 tgctttcggc gattgtgcaa gtcttacaag tataacaata ccggacagtg tgacgagtat
     4021 aggtaatgaa gctttttata attgcacaag tattacaaaa gtgacaatac ccgacagcgt
     4081 agtgagcata ggtggattag ctttcagtga ttgcactagc ctaacaagca tcacaatacc
     4141 ggatagcgtt actattatta atgattttat gttttctggc tgtaaatcgc ttaatgatgt
     4201 taatataccg gatggagtta catatgttgg tgaaggagca tttagtagaa ctgctatatg
     4261 caataatcag accgaagagg taaaatatat cgataaatgg cttgtcgata gcgatagtag
     4321 tgttactgat gtcgctgtaa aggatggaac agtaggcata tcatgcggag cattttcgta
     4381 ctgcgggcaa ttaaaaaaca tcagtgttcc gaacagtgta aaatatgtgg gaggaaaagc
     4441 tttttataat tgcatttcat taacaaatgt agttttacca tacggcatta caaatattgg
     4501 ggtgagtaca tttagtggtt gtgaatcact cgccagcgta acaatacccg atagtgtgac
     4561 gagtataggt gagcatgctt tttttaatac tgcgttactg aaaaatcaaa caacaactga
     4621 aaaatatgta ggcaaatggg ttatagattg ggatagtgat gttaaaaatg tcacaattaa
     4681 aaatggcaca gtcggtatag ctgatcacgc atttgagagg agaacctcac tggaaagtat
     4741 aacaattcct gatagcgtgg aatatattgg tgtgtatgca tttcttgatt gttgggagtt
     4801 aacacaagta aatatgcctg aaagtatgac tactattgga gattgtgcat tttacggttg
     4861 tgattctata gaaagtgtaa tagttccaaa aggagtaact aaaataggtg attggttttt
     4921 ttatggttgt gcaagtcttg caagcgtatc tataccggat agcgttatag acatacaatt
     4981 aaatgctttt gagaaatgtg ttgcgctgaa tgaaataaac gttgatgaaa tgaataaagc
     5041 atataaaagc gattcaggtg ttttactaaa taaaaatgaa actgaattga ttttatatcc
     5101 ggcaggaaag aaagaaacta cttacaatat acctaccagt gtggttacta taaatcgcag
     5161 tgcttttgaa aatgttagtg cgctagaatc aataataatc ccaaatagcg ttgttaacan
     5221 nnnnnnnnnt ttacaattgc aaattactta caagcgtatc aattccgtta agcgtaaaaa
     5281 atatcggaga gtacgcattg gggtattatc aaggagaaca ctttgaaact tcaaagattt
     5341 cgaatttcaa aattgaatgt tacgctaaaa caattggtga gcaatatgca ttcgataatg
     5401 gatttgaata tacccttata ggacatacac ataattttaa taaatgggta attattagag
     5461 aaccaacctg tacggaaaac ggaataaata ctcgttcatg cgatggttgc cttaaatttg
     5521 aaacaaaaac aatcaatgcg ctcggtcatg attattcaac aacttggact acagatatgg
     5581 ctgctacctg cggtgcgtca ggctcaaaat cgcaccactg cacacgttgc agtgataaaa
     5641 aagatgtaac tgctatacct gcgacaggta accataaatt cggtgcatgg agtattattc
     5701 agtctgcaac ctgcacgaca acaggtacac agacaagaac
//
LOCUS       DAWAOG010000059         5603 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1916_length_5603_cov_2.566330, whole genome shotgun sequence.
ACCESSION   DAWAOG010000059 DAWAOG010000000
VERSION     DAWAOG010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1916_length_5603_cov_2.566330"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3509..3518
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1677 a         1183 c         1354 g         1379 t
ORIGIN      
        1 atcgagggca gtatagagtt cgtaagggct aaactgctgc ctgtgtttga aaaggcgctg
       61 aaagcaaatg aatgagataa acggcgttat atgcataaac aagccgcagg atttcacttc
      121 gtttgacgtt gtggcaatta tgagaagagc cgcaggaaca agaaagatcg ggcacggcgg
      181 aacgcttgac ccaatggcaa cgggcgtttt accgatatat atcggcagag cggcaaagac
      241 tgcggactta aaccctgtaa gcgacaagaa ataccgtgca gcattcaggc tcggcataac
      301 gaccgatacc gaagatgtat ggggcaaggt gcttacagag aataacaaga cggtgatact
      361 ttcggaaatg accgaggcgg taaagtcaat gcagggcgat atcatgcaga caccgcctat
      421 gtattcggcg gtcaaggtga acggcaagcg actttatgac cttgcaaggc agggcataga
      481 gatagagcgc gccgcgcgtc cggtcactgt ctattcgata ggattgtcgg attatgacac
      541 cgaaaaacgg acaggcattc ttgatataca ttgttcaaag ggcacatata tccgtacaat
      601 cataagtgat atcggaaaga agcttggcac gggtgcaata atgacctcgc tgtgccgtac
      661 tatggcggcg ggctttacgc ttgatgactg ccacgatatc gaggctctgc gcaatatacc
      721 gcctgaggat acagcgaagc ttgtgttgcc taccgagcgg gtgttctcat gctatgacga
      781 ggtacagctt gacgacaaac agaaaaagct gtttatgaac ggcatgatac ttgactgcgg
      841 aagaatggat atttcatacc cgcaggggac ttttctgcgc cttaagcacg gagatgtgtt
      901 cgtaggcgtt gcaaaggtga acgaggaaaa cggactaaaa agcgtctatc tcaatatatt
      961 atcttaaatt ttcccatgta atgcgggaca cggaggttta ttcttgattg acattaccaa
     1021 aaatataata cctatcccgc cgacagcgac agcgctcggt ttttttgacg gtctgcatct
     1081 gggacatatg aaggtcgtgg aggaatgctt caggcatgac ggactttccc ccgcaatgtt
     1141 cacgttccac agcgataccg cactgccgaa acgggaaaag atagaaaatc tgcttacaaa
     1201 cgaaatgaag cttgaaaagc ttgccgaagc gggtgtcaaa tatatctatt cgcccgattt
     1261 caactcggta tgtaattata ctgcacggga ttttattgag aaaatactcg ttagtataat
     1321 gaacgcaaag gttgttgtgt gcggatttga tttcagactc ggagcaggcg gcggaagcaa
     1381 cgcggacgag cttaaggaaa tgtgccgtga gtacggcatc gaagtcgtta ttataccgcc
     1441 gttcagtctt gacggttgtg tggtacattc aacggcgatc aaaaatctga taaaatcggg
     1501 agaaatagca aaggcaaata agctcctcgg atacgatttt tctatagagg gcaaagttat
     1561 agagggcaac cgcataggca gaaccatagg aagccccacg ataaaccagc tgtttcccga
     1621 taacatagtt atgccgaggt tcggtgtgta taagagtgtg acatatcttg acggcagagt
     1681 tatgccgtcc gttacaaatg taggcgtaaa gcctactgtc ggatataaga acagtcccct
     1741 tgccgaaaca catataatgg actatgacgg cgacctttac ggcaggacac tgcgtgtatc
     1801 gttgcttgat tttatccgtc cggaaagacg gtttgaaagc gtggacgagc ttaaaaatca
     1861 gcttgataag gacaaaataa cggcgaagca ataattacac cgtactgtca cacaggtttc
     1921 acctgtacaa tttataatat aacagatatg cgcagagggt atttctctgc cttaatactt
     1981 cacttgaagg agcagcaaat gatcgaagta aaaaatctga ctaagcgtta cggctctaaa
     2041 acggcagtca gcaatgtttc ctttaccgct gaggaaagcg agatactggg atttctcggt
     2101 cctaacggtg ctggaaaatc aactacgatg aatatcctta ccggttatct ttcctcaaca
     2161 gagggcgagg caaaaataaa cggtatagac attcttgaag agcctgtaaa agcaaaacaa
     2221 atgataggat atcttccgga gcagcctccg ttatatcttg atatgacggt aatggaatac
     2281 ttaaagttcg tttacggact taaaaagtgc aagcttccga taaactcaca tctcaaggag
     2341 atatgcaacc ttgtaaagat aaccgatgta aaggatcgtg ttatcaagaa cctgtcaaag
     2401 ggctacaagc agcgtgtagg tcttgcacag gcacttgtgg gcaatccgcc tgtacttatc
     2461 ctcgatgagc ctacggtcgg tcttgaccct aagcagataa tcgagataag agcgctgata
     2521 aagaagctcg gcaagaacca cacagtcatt ctttcaagcc acatactccc cgagatccag
     2581 gcggtatgcg acaagatagt tatcatcaac agaggtaagg tcgttgcaaa cgacagtgca
     2641 gacaaccttg cgagaaatat gtcggctgac cacaaactgg ttatccgctg tgagggtgct
     2701 cctgccgatg taaagaagct gctttcgact attcccggca tggacgaggt gttcgtcaac
     2761 agggagaatg ttgaagaggg tgtaagtgag tacttcctca atgcaaagcc cggtacggat
     2821 atccgccgtg aggtttcaaa gcgccttgcg tcaagaaatt acgctctgct tatgatgaag
     2881 tcgagcgagc ttacgcttga agaagtgttc cttaagatca ctacaggcga tatttacggc
     2941 aatgtggcag acagcgttga caagctgaag cttacaaagt gatcggaaag gcggaataag
     3001 aatgtttgca atattcaaaa gagaactgag agcatatttc acatcgccgc tgggctatgt
     3061 gttcctggct atattctatg cattttcggg actgttcttc tatatattcg cactgtctgt
     3121 aggctctacg gatatatcaa gcgtattcct gatgatgttt atggtgctta tgatatttgt
     3181 tcctctgctg actatgcgtc tgctttcgga ggataagaaa caaaagaccg atcagctgac
     3241 gctgactgcg cctgtaagtc ttttatcaat agttatgggc aagttccttg ccgcatatgc
     3301 tatatttgct atcggcgttg cagtaatgcc ggtctacggc tttgtaatgt cgacctttgc
     3361 cactgtatca tggctcccga tatggggcaa cacggtagga cttttactgc tgggcggtat
     3421 atttgtttct atcggactgt tcgtgtcatc gcttacggaa aatcagatga tcgcggctat
     3481 aggcggcttc ttcatcaacc tgatgatann nnnnnnnnct gatgatactg cttatgaaca
     3541 cactcacaag cgcactcccc tcaggatttt tccgggatgt actttcaagt atctccgtat
     3601 attcaagata ctccgagata accaacggaa tattcagtct ttcaagcctg atattcttca
     3661 taagtgttat cttcatcttc ctgttcttga ctgtaagagt gcttgaaaag cgccgctggg
     3721 cataagcatt gactacgaca aaggagaaaa cgcatgagta aaattaacga tgcggctgaa
     3781 aacgcaaagg aaacagcggc agaaacaata agctaaagta cggcggactg tcgatactct
     3841 ttacagttat atttatagtt gcggtcgtac ttgtaaatgt aataattaca ctgctcggcg
     3901 acagatttat gcctgccgca gaccttacgg actcaggcct ttacagcatt gagcagtcaa
     3961 cggtcgatta tatcaaaacg ataaccgacg aggtcacaat tacggtgaca agcgaggaat
     4021 ccgctttcac gggcggaagt tcatactact accagacaaa tgagatatta aagaagatcg
     4081 ccgcggcaaa ttcaaatatc acattacagt atatcgatgt agtatcaaac cccggcttta
     4141 tagcaaacta cacagaaacc ataacctcaa acgaaattat ggtcgagagc aagaacacaa
     4201 agcgtgtaaa ggttctgaca tatgaggact tcctcagcat aacctataat caacagtatc
     4261 ttaactatta tggagtaaaa cagcccgaaa aggttgaagc caacgcagag caggctgtag
     4321 tatccgctat tatgaacgtt accgatacag atcctgtaaa ggttgcggtt ttaacgggct
     4381 acggagaaac agagaacact gttttacaga atctgctcaa gacaaacagc tacgttatcg
     4441 aatcggtgaa cattacgctt accgacaaga taagcgagga ctacgatttt gtatttatgt
     4501 tcggacccga taaggattat tccgttgccg atatcagcaa gcttgataca tggcttgaca
     4561 acagcggcaa tttcggaaag aatttggtat atgtaagcaa tcccaagctc ggcgagtcac
     4621 cgaaccttga cggtctgctt gaccagtggg gcttaaaggt cgaaaaggga attacctatc
     4681 agacagatga gaattacaca tacagcggta tgaacacata tcaggtgctg tctgtgccgg
     4741 ataccgattt cagcaaattc acaaacaatt cgcctgttca cggctataat atgagtgctg
     4801 taacatcaaa gtgggagaat cagagcggta acggtaatat ttctatccag tcgctcctca
     4861 aaacatacga tggcgcggtt atcaagcctc aggacagcgg cgacaactgg acacccgaaa
     4921 gtgacgcaaa aagaacacag tattctgtta tcatgcaggc tgtaaagaca agatttgagg
     4981 gtacaacacc gttctcaagc cgtatagtgg caataggcgg tatggaattt ctcaacagct
     5041 cattcttaca gacagcgtct gtaaacaact cacagcttat catgaacatc tttaatgtca
     5101 gctgtaacaa ggaagaggga ataacactca ctcccaagtc ttataataca tctacatttg
     5161 agataacaga cgctcagaag aacggtcttg ttgtaggatt tgtaattgtt ctgcctgtac
     5221 ttctgatagt attcggcata atcatctggg taagacgtct gcataggtaa gcgaaaggtc
     5281 agcaatgaag aaaaatacaa aaataatact cggttcagtg atcggtctgg cggtactcgg
     5341 tgcggcaacg ctcgcattgg tgctgaccca gccgaaggat gatactgccg attctgatac
     5401 gtcaagcgat acatcggtca gcatcaccga ttacgaaacc gatgatattt caacgctcac
     5461 tgtaacaaat gagagcggag aatatacgat aaatcgttta ggtaaggaaa aatggggcat
     5521 tgattcgatt ccggaggcgc ttgcaaacag cagttcgtat tcaaacgcta tgagcagcgc
     5581 cgggggcatg agcgcaaagc agg
//
LOCUS       DAWAOG010000060         5544 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1931_length_5544_cov_2.018036, whole genome shotgun sequence.
ACCESSION   DAWAOG010000060 DAWAOG010000000
VERSION     DAWAOG010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1931_length_5544_cov_2.018036"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2288..2297
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4453..4462
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5074..5083
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1514 a         1153 c         1220 g         1627 t
ORIGIN      
        1 cctcatatac catccagccg aggttcgaat aacctgtgac ttcggggcgc gtaacagtgt
       61 agttttcgtc tatcgttatt gtaacgggat cgtctatctt tatctcgctc ttctgttgtt
      121 cgttccacga aaggaaatcg aatttcttgc cgtttacctc aacatctgcg gtgcagtttt
      181 ctttatcgca gttagcttca tatccgagaa gtgagcctgc actgcttccg tttaccattc
      241 cgctgacgtg gcagttttgg taatttccca taatagcctg acccgtgaga atgccgacat
      301 tgtcatcgcc tgttacaatg gcattttcaa tatcaagatc aaacacaccg cataccgttc
      361 cccagccgat aaagcctgcg ttgctgtagt ccgaatatat cgtcatatac gttatcttgt
      421 gattttcgcc gtatacacaa aatgaaaacg gatggtctga attgccgcct ccgctccagc
      481 ccataggcgc ccatttatag tcgtaaagat caatatcggc tgtaagcaca agcgaaatct
      541 gaccctgctc tacaggctga gtgttcacat agtaaacaaa gctcgcaagc tcctgcggcg
      601 ttcctgcggt aaaatatccg ttttggacgc acttgtcgat atgatcctta tccgcaaggg
      661 ataatatgtc gccgcattcg gcgttgttca gccagttatc gttatcggga taggcgctca
      721 gcagatcggc tttttctttt ttcagcttct gcatttcctt tgctcttttt atctcatcgc
      781 tgttttcacg gtagaagaga acctgcggat agcggtcgct gctgtctgcg aaaacggcaa
      841 gtatatcgct tccgtacata acgatactga acaaggtatc atcgcctgaa taaacattta
      901 taatatcgct cttgtatttt gcggatatgt cataggcctt tcccgaaagg ctgtcgaatt
      961 tttcgccaag cacctcttta aaagcattgc ctgttacggt gagcgaattt cctgcgaaga
     1021 atgtgagtgt tgtgttcagg tcgtcgggat ctacccatac gccgttaata gcagaacctg
     1081 catcggtcag ctcattgtat tcatcactgt cttttcttac aagttctacc gatgagatat
     1141 acttgccgta tacgttatac aatttaagct tttcgccatc aagtcttacg aacgcgttgg
     1201 catcttcaag taaagcagat cgcagcttta caccgtcaag tcctactgtg tatgcatcat
     1261 cgccgctctc acttttgagc attgagagaa aattgtattt ctttatattt atcgtctgcc
     1321 aataggttgt gttacacgtt acccattcac cgatgaccga gttttcaaaa ttttcggcaa
     1381 gctcttgccc tttaacgctg tagacgtgat atgctgtata gcgtataccg tcacagctga
     1441 tttctatcgt atctgaatct ttcgctgtga tacaggcttt ttttatttct tcatcggaag
     1501 aattgtctga ggttcggtcg ctgatctcga taatatccaa ttcgtcgaat attgccgttc
     1561 tgcctttatc ggtaaagttt gccacaaagc cgttttcatc gatccatgtc ccacttagaa
     1621 atttatcggt gtccgaaagc tctcccgacg gaatttctgt tacggtgcgt aaagactttt
     1681 tagtttcgga attatcggct ttgctttcgg tttcggacac ggagctgttt tgcacgttcg
     1741 agtcagggtt atctgtgctt gttgttctgc cggattggca cgaggtcaat aataaagatg
     1801 tgattattgc tgtggttatt gcggtaggta aaaacggatt tcttttcata tatattcctt
     1861 tctgaaatga aatgggtatt tcgtgtggtg atgttcattt gctatacata attttaccat
     1921 ataacaaaga ttattacaat agaaaagaag aaattttatt ataaaagttc aagcttgcga
     1981 ctgccttaaa atcgataaaa tctgagatat ccgtcattcc cgataaagca gtatacgcaa
     2041 ggattcaggt gtgatacctc aaagtaaaca aaagcatatc cgacaacaca cattttatat
     2101 actgctaagc ggaatatctt tgctaaattt acttttgcaa ttacttaaaa ttgctaaccg
     2161 cagcctctgg aatttactta aaccataact ccgagaaaat atttcaatta ttttaaaaaa
     2221 cccccttgac agcctgccga cattgtgcta aaatacccaa agcaaaaccg atttttccgt
     2281 gtaatatnnn nnnnnnnatc ggctttacaa aaagaaatag agcctgcgca gagcgtttga
     2341 aacacgattt tgcacagaaa tatgaaaacg attaccaagt tctgtcgaat gttaggtaaa
     2401 tatttttacc tttatgatga ccgacagcgg gaaaggtgtg gtgaaatgtt ccggtttaaa
     2461 tggatgctcg gaaagatgaa aggctaccgt gcggtttacg ccgtagcact gtgtctgtca
     2521 gtggtgatac agtcgttttt ccttacagga cctatgattg ttgagcagat aataagtata
     2581 tttatatctt ctccggacgc tttgcagaat attgaaaatc agcgcgatct gctgatactg
     2641 ctttgcgttg cgatgatctt attcgttttt gttcgtacct gtctgcaatt ccttgcaaaa
     2701 actctttatg agatagtatc acaaggcgtt ctgctaaaag tacgaaacga gctttttgag
     2761 cgtattcaaa atcaagatat gcacttcttc gacactcata caaagggcga tcttgtaaca
     2821 aggcttacgg gcgaccttga tatgatacgt cattctatcg catggataat tatgatggtg
     2881 gttgaaagcg gtgcattgtt cttattcacc gtgatctact tttttacgat tgactggctg
     2941 atgacgcttt gcatacttgc gcttactccg atactcttta tagtgactcg tatgtttgcg
     3001 aaaagagtag gaccgaaata tgtagtgctg cgtgaaaagc tctcacgact taacacaaag
     3061 gcgcaggaga atatcgccgc aaaccgcgtt gtaaaggctt ttgcccgtga ggattacgaa
     3121 aaagaggact ttgacaagta taacaaggat tactgcgagc ataatcagca ggcggctatg
     3181 gtatggctta cattccagcc ttacatagaa acactggcgc aggctctctg ggctgtgcag
     3241 atgcttgtcg gcggcatatt tgttatcaac ggaagaatta caattgctca gtacacagta
     3301 ttctccggac ttctgtggac aatagcaaat gctatgagaa atatcggtat gctgataaac
     3361 gatttacagc gtttctttgc tagtatttcc aagattatcg aggtatatta ttcacgcccg
     3421 aatatcgtga acaatcataa cagtgccgaa cgtccccgtc ttaaaggcga tatcgagttt
     3481 aagaatgtat cggcaaagtt cggtgatatt actgtccttc acaatatcga tcttaacata
     3541 aagcacggtg aaaccattgt aataatgggc tctacaggtt ccggcaaaac aacgcttatg
     3601 aaccttatcg ccaggtttta tgatgtgtcg gagggcggag tatatgttga cggaaagaac
     3661 gtaaaggatt acgaacccga tgtacttcgt gcgaatatcg gtatgactac tcaggaagtc
     3721 ctgctgttct ccgatacggt tgacggaaat atagcatacg gtaagtcgga tctgcctttt
     3781 gaagatgtaa agaactttgc tcatttagcg tccgcaacat ttatcgagaa gatgcctgag
     3841 cagtacgaaa cgataatagg cgagcgcggt gtaggtctgt cgggcggtca gaagcagaga
     3901 atagcgctgg cgcgtgctat ggctatccgt ccgccgatac tgatacttga cgatactaca
     3961 tctgcacttg atatggaaac agaggcagag atacaggaaa acctcaataa tcttgatttt
     4021 gagtgtacaa agctgattat agctcagcgt acatcctccg caagatacgc agacaggatc
     4081 attatccttg aggacggtcg tatcaaggag tgcggtacac acgatgagct gatgaagcaa
     4141 cgtggatatt actatgaaat atataccctc cagaacggta cagaagagga ggcggtttaa
     4201 atggcaagaa ataaattcga cgttgatgaa acacttgaaa caccatttga tttcaagcat
     4261 ttcaaacgtg caatggttta tgtaaagcgt tataagtgga agatgatact ggcactttcg
     4321 ctgtctgcaa tatcggcaat agttgcgctt atcggccctt tgcttacaaa agaggctgtc
     4381 gatgtggcga taccggacgg cgatattcct tatatagtat ttttgtcgat aggctttttg
     4441 ctgtctataa ttnnnnnnnn nnaattgtca gcgttgtgct cggaaatatc cgtcagcgca
     4501 ttatgacaaa agcgggtcag cttatgatat acgatataag aaaagacctg tttgcgcatt
     4561 tgcaggagct tccgttccaa tattatgacg atagaccgca gggcaagata cttgtccgtg
     4621 tcgtaaacta tgtaaataat gtatcggata tgctgacaaa cggcataatc aacttcattc
     4681 ttgaaatatt caaccttata ttcataacga tctttatgtt ctcggtcgat gcaagactgg
     4741 cgcttgttat ctttgcgggc gttcctgtat ttatcgttgt tatgataata ctgaaaaaca
     4801 agcagagaag ggcatggcag ggggtttcaa acaagaactc aaaccagacc gcataccttg
     4861 ccgaaagcat aaactgtatg cgtgtaacgc agagctttgc gagggagaac gaaaacctcg
     4921 gcatattccg cagactgtct accgcctaca gaaaggcgtg gatgaaagcg gtcacggtaa
     4981 acaactttgt cagcttctcg gtcgataata taagagcggt ggtagactcg gcactgtact
     5041 ttgtcggact gctcgttata ggctatcccg cggnnnnnnn nnnattcctc acgcttctgg
     5101 cagcctataa tcaatatcgc cagcctttat aataactttg taaatgcggt ggcttacctt
     5161 gagcgtatat ttgaaacgat ggacgagcct gttaacgtca aggatgccga ggacgcttac
     5221 gaaatgcctg atatcaatgg tgaagtcgaa taccgcaatg taacattcgc ttatgaagaa
     5281 ggtaaaaata tccttgaaaa tgtaagcttt aaggtaaagc cgggtgagag cgtagcgctt
     5341 gtaggaccta caggtgcggg taaaactact atagtaaacc tgctctcacg tttctatgac
     5401 cttacaggcg gcgcagtgta catagacggc aacgatataa gcaaggttac gcttcattcg
     5461 cttcgcgata agatgggcat gatgatgcag gacagcttca tattctccgg tactatagag
     5521 gataatatcc gttacggcaa gctt
//
LOCUS       DAWAOG010000061         5538 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1934_length_5538_cov_2.539850, whole genome shotgun sequence.
ACCESSION   DAWAOG010000061 DAWAOG010000000
VERSION     DAWAOG010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1934_length_5538_cov_2.539850"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2566..2575
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1779 a          923 c         1354 g         1472 t
ORIGIN      
        1 ataggtgagg gtttcaagct gtttcagaag aaatatcagt ttaagaaatc gctggttttc
       61 acgcttgttt atcttatagc gctggggctt ggcatagatt ttctgataag agatcatacc
      121 ggtatctacg gttatattct ggttggtctg tcgctcggta tgatattcta tacctggtac
      181 aagcctgtgc ttataagaaa aaagctgata gcgactataa gctcacttgc cgaagaaaca
      241 tatacggctg agttttatcg tgacaggata cagataacga ccgtaataga agaacctgtc
      301 atagcggaaa atgataccga aacggaagaa gaaaacgata ctcccgcaaa agatgaacct
      361 cacgaggagg aagaacagga agaagtagtt acaaatcttt attttggctc tgattattta
      421 gacgctatgg aaaatgagca gatgttttta ctctttgtaa acagacagaa tatttatatt
      481 ttctcaaaga aatgtctttc cgaagaagaa cagaacaagc taagagaaat atttaccgaa
      541 aaatcaatac tctgattgat ataaataaat cggaaaggaa atcggaatgg aaaaagacgt
      601 taaagaattc agtacagcca ctgaaaagct gatacaggta gacatagaaa aggaaatgcg
      661 tgaaagcttt ttgcagtatt ctatggcggt gctggtatca agagctttgc ctgatgtaag
      721 agatggtatg aagccggttc acagaagaat aatatacact atgaatgagg ctgacaatac
      781 atcaagcaag ccgtacagaa aatgtgctta cacagtcggt gaagttctcg gtaaatatca
      841 ccctcacggt gacgcatcgg tatatgatgc actggtaaga cttgcacagg atttctcaat
      901 gagatatccg cttatagacg gacacggaaa cttcggttcg gtagacggcg ccccgcctgc
      961 cgcttaccgt tatacagaag cgagaatggc aaagatagca tccgagcttc tgaaggatat
     1021 aaagaaagac actatcgact ggggcaagaa ctatgatgat aagctggacg agcctacggt
     1081 tcttccggta aagtttccta accttttagt aaatggctct gtgggtatag ctgtaggtat
     1141 ggctaccaac atacctccgc ataacctgaa tgaagtatgc gatgctatag ttgcaaggat
     1201 ggaaaatccc gaatgcggta ttgaagagat acttcagtat ataaagggac ctgattttcc
     1261 taccggcggt ataataatgg gctatagcgg aatacgctcc gcatattata caggtcgagg
     1321 caagatcacc ttaagaggta aagctgagat agttgaagag ggtaaccata caagaattat
     1381 cgttaccgaa attccttata tggtgaacaa gtcaaagctt cttgaagtga cgggacagct
     1441 tatgcgcgac aagcgtattg agggtatttc ggcgcttcgt gacgaaagtg acaaggacgg
     1501 tatgcgtata gtttacgagc tcaagagaga tgtaaatgcg caggtcgttc ttaataagct
     1561 gtattcattc acacagctcc aggatactgt cggtgtaatt atgatcgcac ttgttaacgg
     1621 cgagcctaag caactgacgc tgcttgaaat tctggataac tatattgctt ttcagaagca
     1681 gataatcaca agaagaacag catttgatct aaagaaggca agagagagag cacatatttt
     1741 acagggcttt ctgcttgcta ttgataacat tgacgaggtt atttccatcc tgcgttcaag
     1801 taagtctgta caagaaggta aagaacgtct gatggagcga tttaaggaag acgaccttgc
     1861 taagctgttg cagagagcta tgggagagaa ctataaggat gttcattttg aacacgagat
     1921 aggactttcc gaggagcagg cagatgccat cgtacagatg agattaggac agctgacagg
     1981 acttgaacgt gacaaggtta tttccgagct tgcagaaatt atggagaaga taaacgactt
     2041 ccttgatata ctttcaagcg atgagagagt taaggagata atcaaggaag aaatcacggc
     2101 aattaaggaa aaatacggtg atgagagaag aaccgctatt gagcctgtaa gtggtgaggt
     2161 tgatattgag gatcttattc ctgaagaaga gtgtgtactt acttatacta atatcggata
     2221 tatcaagcgt cagcctgtag atgtatataa tctgcagaag cgtggcggta agggcgtatc
     2281 tggtatgaag cagagggaag aagactatgt tgaggatatg ttcataagct ctacgcacga
     2341 caatatccta tttattacaa attatggtaa tatgtataaa ttaaagtgct acgaagtacc
     2401 tgagggttct aagcagagca ggggaacaaa catcgtaaat ctgttacagc ttgatgaggg
     2461 tgaacgtatt gcggcaatga tgaagacaag cgactttgcc gagaacaagt actttatctg
     2521 tgttacaaag tttggtaaga tcaagagaac accgctttat gatttnnnnn nnnnncaatt
     2581 acgcttgcag agggcgatga aatagcagcc gcacatctta ctgagggtga cagctcgatt
     2641 atagttgcta cacatcttgg tatggcaata catttcagtg aagataaaat aagaagtatg
     2701 ggaagacttg ccgcaggtgt aagagcaata aaactgcgtg aggacgacta tattgttggt
     2761 gctgccaagg tatacagtga tgatatgaga atacttaccg tgaccgacaa gggatacggc
     2821 aggctcagtg cactgagtga ttaccgtatg cagaacagag gcggtaacgg tcttaagaac
     2881 tacaagataa gagatgacag aggatatgtt tgcggtatac gttctataca ggctgacgat
     2941 gatgttatac ttatcagtgc agacggtgtt attatccgta tccgtgccaa cgatctcaga
     3001 gtaatgagga gaaccggttt aggtgtaaga gttatgaagc tgtctgacga ggatagagta
     3061 gtaacattta caagaaccga acatgatgaa acagcagata tagaagaagt tgaacaggca
     3121 agtgatgaag aaatagcaca ggcagaggct gaagctgctg ctgaagttat cgaagacgag
     3181 cctgaaacaa acgaagaaga ataaaatata tcagcctgtc ggcagaaatt ctgtgacagg
     3241 ctgattttct gaaaaaatac ctattgttcc acatagaaca tttttatgat cggggttatt
     3301 tatgtacgaa cttgattatt atgacgttat agttgtaggt gcaggtcatg ccggttgtga
     3361 ggcggcactc gccgccgcaa gacttggatg taagactgcc gtttttactt taagtcttga
     3421 cgctattgca aatatgcctt gtaatccgtg tataggaggt tctgccaaag gtcagcttgt
     3481 aagagagctt gactcgctcg gcggtgaaat gggtagggcg gctgatgcta cttttataca
     3541 gtcacgaatg cttaataagg gtaaaggtcc tgctgttcat tcgctcagag tacagagcga
     3601 cagagtaaag tatcatactt ttatgaaatc tgtccttgaa catactgaaa accttgatat
     3661 caaacaggct gaggttactg aggtttgtgc cgagaatggc aaaataacag gtgttaaaac
     3721 aaggctaggt gctttttatc ccgcgggttg tgttataatc actacaggta cttatctcgg
     3781 aggtaagata catataggtg agcttaatta tcaaagtgga ccggataatg tatccgcggc
     3841 tctgcaactg actgacagcc ttaaaaaatt gggtctttct atgagaagat ttaagacagg
     3901 aactccggcg agggttcata aaagatcaat tgatttttcg gttatggaag agcaggaggg
     3961 tgatgagtat atcactccgt tctgctttga caacacgttt aagcttgaaa ataaggtaaa
     4021 gtgttatgtt acttatacaa acgctgaaac acataggatc attcttgaca accttgacag
     4081 gtcgccgctt tatgcgggaa gaatacaggg tgtaggtccc agatactgtc ccagtattga
     4141 ggataagatc gtgaggttct ctgataagcc gcgacatcag ttgtttgttg agcctatggg
     4201 actcaataca gatgagtatt atctccaggg tatgtcaagc tcacttccgg aagatgtgca
     4261 gattaagttt ctcagaacaa ttaagggtct ggagcacgtt gaaataatgc gtccggctta
     4321 tgctatcgaa tatgactgtt gcgatccgct tgagctttat cctacacttg aatttaaaaa
     4381 gatatccgga cttttcggtg cgggtcagtt caactgtaca agcggatatg aagaagccgc
     4441 cgcgcaaggt cttattgccg gtatgaatgc ggcaatgaaa attaagtgca gagaacagta
     4501 tattcctgat agaacaactt ctttcattgg tacgttgatc gatgatcttg taacaaaagg
     4561 ttgtgttgaa ccttacagaa tgatgactag cagaagtgag tacaggcttt tattaagaca
     4621 agacaatgct aatgaaagac ttataccggt gggtcataaa ttcgggcttg tttcagatga
     4681 gcgctatcgg aaatttcttg acagaaaaga aatgcttgaa aaggaaaccg agagaataaa
     4741 gaaagctact gtttatccca gtgaagagct taacagtatg cttgaaagta aaggaacatc
     4801 acctattaca cagggtatca agtttattga attgttaaag cgtcctcaga tcgattacag
     4861 ggatctgata aggtttgatg aaaatgcacc ggagcttgag catgatataa tagataaact
     4921 tgaaatcaat atcaagtatg agggatatat taagacgcag actgaaaaaa ttgagcagat
     4981 gaaacgtctt gaagagaaga agttacctac ggatattgac tataagacaa tttcaggact
     5041 gagaatagag gctcaggaaa agctgaataa gtataagccg ctgaatatag gacaggccgg
     5101 cagaatttca ggtgtaaatc ctgccgatgt gtctgtactt ctgatatggc tgtcagggag
     5161 aaaacatgga gaaaattgaa tttatcctga atgaatttta taaatgtgat attaagctgt
     5221 ctactgagca ggcagagagg tttaacaagt tatatgaatt tctggttgag tacaacaaaa
     5281 acgtcaacct gactgctata accgatttta aagaaatcgt agttaagcac tttatagata
     5341 gtgttctacc attcaatatg attgatattg aagaaaatag cagttttatc gatgttggaa
     5401 ccggagcggg ttttccttca attccgctta tgatagtaag acctgatttg aaatgtacga
     5461 tgcttgaagc tctcaataag cgctgtgttt ttcttgaaaa ggcgtgtgag cttgtaggag
     5521 tcaaggcaac agtagtgc
//
LOCUS       DAWAOG010000062         5504 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1949_length_5504_cov_1.981281, whole genome shotgun sequence.
ACCESSION   DAWAOG010000062 DAWAOG010000000
VERSION     DAWAOG010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1949_length_5504_cov_1.981281"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    762..771
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2074..2083
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2889..2898
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3326..3375
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1589 a         1111 c         1219 g         1505 t
ORIGIN      
        1 ataaggctgt acatacccgt acctactctg aaatactaca aaaagcaacg gtgcaagatt
       61 gtttacagcc gcctggcata tataacctat ataacaggcg tttaccgtgg agcgatgtgt
      121 tatgttttta gtcattaaga aagtccttta aacgaaaaaa tccgctttat tataagcaat
      181 cgaccgagtt ttttcaatcc caaaaacaat aattataaaa acgattacac aaattcgctt
      241 ttttgcacaa ataacggctt attatattag tcaattctaa taaaatcact acaaacgcat
      301 ttactaaccg cttaaaatat gctataatat tcggagaatt agtatggcca tcgtccgcaa
      361 aggataggaa tataccgtaa aggcgaggga aaggttggtt attttttatg aagatcaaaa
      421 gcttattgat catagtgctt gcttgctttg tgatcattcc gtcggttgta tttgcgtctg
      481 ttgcaaatgt cgagatgaac aagctcgcac ttaagaacta caaacagact gcacagtcta
      541 tggctgaaaa tcaagcttac aacatatcgg agtatttccg aataatttcg gcaagcgcta
      601 aacagatagc cgacaacccg gatattcttg aatttaacag cgataaaagt cctacttcta
      661 cagacgcact tgacatctat tcaaaaatcg tgtcttctga caatgaaaat gtcagaattg
      721 accgtataat gatagtcaag aagagcgatt atcttcccgt annnnnnnnn ngtctttcaa
      781 aggatgatat actcagcaac acgcttaaga gcatatgtga gagaaaagac ggtgaggtaa
      841 cattctacac tcccgatgtg tacacgacct tcaaagaagg cgacaaggag cgtaatgacc
      901 cgcttttccc cggaaagtcc ggcgcaaatc ttcgtctggt agcaaagtat gctgtcggag
      961 attattatgt agtaacattt ttcaaccatt ctgcactgaa gtcatttgct acttcgtcac
     1021 agtttgccaa caacagtaaa ctcattctta tcgaccctaa cggttcgatc atgcatgaca
     1081 gctacatagg taatacatcg gataataaga acagcgctta ccttaagttt accgacaagg
     1141 cgcaggtagg ctctgttgct gaggtcgata actttaaggg tactgatagc ggcgttatcc
     1201 ctactatagg tttcgcttca aaattacccg ctataaatga gggagaagaa ggaaactgga
     1261 cacttgccat tgtagccgaa accgacaagg catatacctt atcaggtgaa gctatgggaa
     1321 gcataatcgg tatgatcgta tttgtttcga ttattctggt ggcagctgct gtagttcttg
     1381 tattcatact tactaagcca atcaaggtca ttgaagaaac gcttgtcaag gttcacagag
     1441 gtgaccatga agcgagaata aatgtaattg caaacaacga gtatggtcag atcgcccgta
     1501 cattcaacga tcttatcgac gatatcgttg taagtgaagg cagataccgc actatcattg
     1561 agatgtccga taatataatc tttgagtgga atttcaagac taacgatgta ttcttctcga
     1621 acaatttcaa taagaagttc agctatcgcg caccgtccga tcacttcggc gacagcttcc
     1681 tgcttaaggt aaaggttcat cccgaggata acgagcgtta tcacaaggat ctcgaaaagc
     1741 tctcaaaggg tgaagagttt gagggcaacg agtaccgttg gaagaacatc tacggtgact
     1801 atatctggat acttctccgt actgcaacta tccgtgacag agatggaaat attgctaaga
     1861 tagtgggcgt tatcgttgat atcgaccgtg ctaagaagag tgaaaagctc cttaccgaaa
     1921 gagcaagcta cgacagcctt acaggtcttt ataaccgtga atctatcgag cgtaccattg
     1981 ataacgaaat cgagcttatc aatgtccgca agagcgagtt tgctattctg ttcatagatg
     2041 ttgatgactt taagatatac aatgataaat acannnnnnn nnncgctaca ggtgaccagg
     2101 tgcttaagtt cgttgccaat acaataaact tcgttatcaa gggcttcggt acagccggac
     2161 gttatggcgg tgatgaattt gtcgcttgca taagaaatgt tgaaacaaat gatcccacaa
     2221 gagtagcccg tgatatcctg tcaggactta aggagggctt tacatcggat aacggtgaca
     2281 agctctcggt caatgcaagt atcggtattt cgatcatcaa ggattcttca atgcacgttg
     2341 acgagataat cggtatggct gatgacgcaa tgtacaagat caagaagaac ggtaagtcaa
     2401 acttcggtat actcaataag gaaacagtcg ctaagtctgc tcctgctgag gttgaagagg
     2461 aagatattat cggcggcgct gaagaaacga cagctccgac agaagatgct cctgtacagg
     2521 agtaaaaacg tgacaaaata aataagtaca agaggagtgc tgttttaacg gcactccttt
     2581 ttgaactaca gaacagcgag ggataatatg atacgggaag tgcgtgaaga tgaatacgaa
     2641 cagctgatga gcctttattg tcagctccac gaaacaagcg taccggaata taagggtgat
     2701 acaaaggagc tttggcaaag gcttgtggct aaccccgatt atcactttat tgtagccgag
     2761 gaggacggta aaatagtatc tacctgtacc tgtataataa tgccgaatat gacgcacgga
     2821 ccgcgtcctt atgcgtttgt cgagaatgta gtgacagaca gtgaatatcg tggcagaggg
     2881 cttgctacnn nnnnnnnnag gcaaaggaga tagcaaaggc tgaaaactgc tacaggctga
     2941 tacttatgac aggctcaaag cttgaaagta cactgaactt ttacagacgc gcagggtatg
     3001 atgataagga aaagacggga tttatactgc atctggatta acagaaacgc aaatgacctg
     3061 tgtactgaca caggtcattt tgcatataac aaacccctta tcttgcgata aggggtcaag
     3121 ctattcatta cggattaacc gaagatgtta agcagaacac ctgcggcaac ggctgagccg
     3181 ataacacctg caacattcgg tcccatagcg tgcatgagaa ggaagttaga ggggttttcc
     3241 ttagcgccga ccgtctgtga aacacgggct gccataggaa ctgcggatac accggctgaa
     3301 ccgataaggg ggtttatctt gccgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnngagct tgttcatgag ctttgcaagt aacagaccgc ctgctgtacc
     3421 gaagcagaaa gcgatgagtc cgagggcaac gatcataagt gtctttacag tcaggaagtt
     3481 ttcagcagtt gctgtagcac cgacaacagt accgagcatt atagtgataa tgttcatcaa
     3541 ctcgtttgat gctgtcttta caagtctgtc tacaacgccg ctctctctga agaggttacc
     3601 gagcatcagc atacctacaa gaggagttgc atcgggaacg ataaaagcaa cgataagcgt
     3661 aacagcgaca gggaatatga tcttttctct ttttgatacg ggacgtaagc cgtccattac
     3721 aatagcacgt tctttttttg ttgtaaggag cttcattata ggaggctgga ttacgggaac
     3781 gagcgccata taagaatacg cggcaaccgc tattgaaccc agcaattgcg gtgctagctt
     3841 tgatgtcagg aatattgcgg taggtccgtc agcaccgccg ataataccga tagatgctgc
     3901 ttctttgaat gtaaacgcta caccttcaat acctgtaagg ctgaatgcgc aagcaccaag
     3961 gaatgttacg aagataccga gctgtgcggc agcaccgagc agtaagctct tggggtttgc
     4021 tatcagagga ccgaagtctg tcatacagcc gatacctaag aatatcagcg gagggaatat
     4081 ctgaagctta acgccgagat acagaatatc gagcagaccg ccgttgtgaa gtacctcgcc
     4141 gtagtttaca ccgttctcgg caaagttcgc cgctattgcc ggatctgggc ttataccgaa
     4201 catttccggg tggaacatat tagcaccggg aatattggta agcatcatac cgaaagcgat
     4261 aggaagcaac agcagaggtt caaattttct tactatagca agatacataa gtaagaatga
     4321 aacagctatc ataagcccct gctgaggggt tatgttgcag aagcccgact tttgcgcaag
     4381 cgcgacaagg gcattccaga aaacgtctaa aatttccatc ttgttctcct tttatttagt
     4441 ctgagcctta aaacgaacgg gtattctcgt ttacgcccgc attgccccag cttgaacgag
     4501 gctgtttgtc ggcacgtttg attctcttga ttgcataggt cttgccgtcg cctgctgtgt
     4561 aagcagcgat agcagccgaa ataacagcta tgacctcatc gtcgttctcg tcctcttcga
     4621 taagttcaac aggagcggga gcgggtgcgg cttcagcagt cttttcagcg ggcttgtcat
     4681 cttttttctt cttgtttccc agattgcaga agaaggaaat gatgtaaagc agtcctataa
     4741 gcagtcctaa gatcaggaaa acaacaacca taccggtaat gaccgtagcc cacataccgc
     4801 ttatgttctg agcggcagac tcttcggtga ttatcgccat agtacgcagt aaattatccg
     4861 tcatgcatga ttctccattc ttaattgttc tcgctttgct gtgccgtttt ttactgttta
     4921 ctgataatgc ggcatagcat aaaactaata taattatacc ttatttcagg tgtaaattca
     4981 acgcttttct ttgtagattt tttaacaaat tttctttaat aattatgtgt atttttagcg
     5041 taaatttaga taaaatcaca aaacttaagt gaaaagtggg taggggactg ttaatggtga
     5101 tgaaagctta tgtatgtaaa ctaaaaggag ctgtcgtata caacagctcc tttgctcaaa
     5161 tcaatattta tcgttcacgt cttctttaaa ctctacgttt gtctgaactg cggtatcaaa
     5221 gtgcgtaata tcgctagctt tcaacttttt gttttcggcg ggtttgtcct gctgttttgt
     5281 ctgagttttc ttttcagctg tgaagtcggc tgctattggc tttatcgatg aatttgcggg
     5341 tttatcgaca ggctttgtat cagcgctttt aattctgttt tcctttacgg aagttttcgc
     5401 attttcatcg ttttttgttt tcttttccga tactgcaggc tttgcagctg cgcttttaat
     5461 tctgttttcc tttacggaag ttttcgcatt ttcatcgttt tttg
//
LOCUS       DAWAOG010000063         5447 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1974_length_5447_cov_2.037834, whole genome shotgun sequence.
ACCESSION   DAWAOG010000063 DAWAOG010000000
VERSION     DAWAOG010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1974_length_5447_cov_2.037834"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3370..3379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1391 a         1258 c         1138 g         1650 t
ORIGIN      
        1 gcctataata gtagatggtg aacagatagg ctcccttata ggcggtcagg tattgacgga
       61 agcacccgac cttgaaaagt tccgtgagat agcaaaggaa atcgacgttg acccggatga
      121 atatgtagag gccgtaaaga aagtaccgat agtttcggag gaaaaggtaa acaatgcggc
      181 agagcttctt ttcaagatgg ctcaggctct ttcacaggtc ggatacgaaa agtaccgcat
      241 cacagaagaa cacaaggata cttcggcaat gttcgatgag gtccaggaag actatgtagt
      301 tataaacgca aaggtcgaag aagtcaacac ggcaattgcg gcattatccg acgagtttga
      361 tcttcttcgc gaaaaagcaa atgagtctgc taaggcggtt gctcagacag actcgatcct
      421 gaaatacata cagaatgttg ccactcagat gaccttgctc ggcttcaatg cttcaataga
      481 agcaaagcac gtcggcgaag caggcgccgg cttcaatgta attgcacagg aagtacgtca
      541 gcttgcagaa cagacttcca atcagacacg cagtatcgaa gacgtgctcg gtagcgtacg
      601 ctcctcaatt tctgctatcg acaaggaaat tgcaaccgca gtaagcaaga tagaagccaa
      661 tgttgctaca gtaaaggagc tcagtgacaa gatcaccgaa acttccaaaa agatagatag
      721 tatcagcaag gcataaaaaa attcagataa gcaagcagct cgtcgcagga tattctgcgg
      781 cgagcttttt gcgtttatat ggcagcggat aaaaagcaac ggctctgcaa aagcagagcc
      841 gtcattatta ttaagatcaa gaattattca acagtcttgt tgagttctgc aagacgagcg
      901 tctatctgac cctgatactg ttcacggaaa gatgtgtaag attcacctgt catgtacgga
      961 ccctttgtga gctgttctac agggttatcc tggttgtctg tgcttgcgat atcctgaccg
     1021 ataccgttct taaagtcaaa tacgcctgtg aatgccgtgg gatccttcat tgtatcaagt
     1081 ctgtcaagca gtgtatctgt ccagtcatac tcagccttgg actgctctct gcctgccttc
     1141 tgtatttcgg gatcgtttga agcaactaag tttgcataga tccatgcctt gtaaccgtct
     1201 atgttcttag aacctgcaac gagcattata gaatcctgct tcatgctctg ataatactta
     1261 tctgaaccgt ccatctgagg gaagggaacg aagttgatct catcgtcatc ccacttctgt
     1321 tttttcttat acaatctcca ggtttcttcg tatctccatg taccgtcttc aaagaacagt
     1381 gtgttgccgt ttacccactc gggatagctg ggtgcccagt tgttatcgat atctctgggg
     1441 taacggagct gttcctgtgt gttatcgaat gaacgaatca tatcaaggca cttttcgatg
     1501 ttagcatcat taaggtgact tacaagctta ccgccttcaa gtgcgataag aggtgtacct
     1561 gtcgtgcaga caaagttgtt ttcggggttg taaccgtcaa gtacatactt gccgttatcg
     1621 ggatctgaga actttcttgc cattgtcagg aatgtttccc atgtccactc acccttttca
     1681 taaagctccc agggatcgtc aagacctgct tcttcaacag ttgacttttt gtaccacagg
     1741 aagcttgtgg gaacaagact cataataggg cagtagttct tgccgcccca gttgaacttg
     1801 tcgatagctt ctttataatc agcccaatca tctgttgtga aatcaatgat accgtcaagg
     1861 ggctggaaaa ggttctgata tacgctgtaa ggatagttgc agatctcaaa ggggaagcag
     1921 tcgggggaat catctgactg tacgagcttt gaaaggctgg tatatctgtc cgcatagctt
     1981 gaaaccttga cgtttacaaa tacgttttca tccgctacgc tctcatagcc tttaggcttg
     2041 ttctcgggtg tgccgtaaag cttcttgaat acctctactg cgggagaagc ctcctgaatg
     2101 tcccaccatg ccatccactt gatctttgtg tcaaccttga tgtccttata attttcgccg
     2161 gcagaaacat tgttaactgc gctctgaacg ccttcgttct cagacatctg cttctgagtt
     2221 tcgcctgttg tagaagcagc tgagctgtct gttgttccgc ttgttgttgc gggtgaaccg
     2281 ctgcctgaac aaccagaaaa agctgttacg gcagatacga gtgctaatgc gcctgcaata
     2341 gccttggtat attttctcat ctttaatcct cctgtagatt ttctgtacct gagtacagat
     2401 ttgcgtatta ttacaattac tattataaca aaattgctgt cacaccgtca ataaacattt
     2461 tgcagtaatt tagctaaaca attttgtgaa aatagcacaa tcgaagcatg ttaatttagg
     2521 ttaattagct aaatttcagc ttattctgct attcatatca ttcagatatg cctcaatggc
     2581 aggcacatac tcccctttca cttcatcctc cagattcttt ttgtaatcct gaccttccat
     2641 ataacaccag tcgtccatct cgtcgatata gctttcgccg cagtacaaag gattcggatt
     2701 gctgcatatc tgcttaaatt ctataaccgg tacaaatgtt gccggatcgt ttatcctgtc
     2761 cattctgtca agcaatctgt cggtccagcc atattcggat tttgcattct gacgtgcaat
     2821 gttcttttgc tctttgtcac tatttgccaa tgagccggca tagataagcg acttataccc
     2881 ctcggcattc tttgagcctt tcgggaacat aaacgaattg atgattatgc tctgataata
     2941 agtctgttca tcatcacagc gcgggaacgg aacaaagctt atctcatcat cgttccaatt
     3001 attcttattc ttgtagagct gccatgtttc atcatatctc caactgccgt cgttatagaa
     3061 caaagtgttg ccgttcgccc actcagtgta gctgggtgac caattattta caacctcccg
     3121 cggataacgg tagcctttct gagttgttgc aaactgacgg acaaaatcca tggcttttaa
     3181 tatcttttcg tttctgaaat tacccacgag ctttccgtcg ttcagcccga caagaggtac
     3241 gccggtcgtt gtccaaagat atatatctgc gctatagccg tctatagcat atttttcctt
     3301 ttccttgtct gtaaactttt ccgacatttc aatgaacttg ctccacgtcc attcgtcttt
     3361 ttcataaagn nnnnnnnnna agctcccacg ggtcttcaag accgttttct ttgatagtag
     3421 aagttttgta ccacaaatat tccgaaagct tagcgtcata cacaggggca aagttgctcc
     3481 tgccgatcgt tgtcttcttc tgcgcctgct catactgtgc aaattcggcg agtgaaaaat
     3541 caattatatc atcaaccgga gcaaacagta aattgctgtc atggtcaatc gaataatacg
     3601 ggaacattga ttcgcaaggg aaagcgtccg gcgaatcgtc tgactgtatg agctttgcaa
     3661 gatcaaaata cctttctgag taattgctca cctttatact tacaaacggc tcttcatcag
     3721 ccgtactccc cgaactgctc ttccggtatt taattttctc ataccctgag ggtatttttt
     3781 ccggcacacc gaaaagctcc ttatatgtat cggcaacatt gctgttatta aggtcatacc
     3841 agccgagaaa tttcaccttt gtgtcgacct tgatatcccc tataacgtcc ttaacggact
     3901 tgcttgcaga tacagtgtcg ttactgctgt cggtgctgtc ggtgctgtcg tctgtttccg
     3961 cattttccga agcggcgggg tcgctgtcct ttatttctgt actgctgtcg ggagatgtac
     4021 tgcccgagca ggcggtcgtc agcattaccg ctgacaggat aattgcggat agcttctttg
     4081 tgtgtttcat aaatagctcc tttgatgtag tgtgaaatga attttattgc tgagttacga
     4141 tattttctgc tgatttgttg atgctgtctg ttgctttatc aagctccttg ataatgtccg
     4201 gtctttcttc ggtaataaac gttctgtagt ccttgccgtg cacatacgag ctttctgaaa
     4261 gtctgtcaac aatgacatcg gtcaatgcgg taggcggtac ttcacggaaa taatcacggc
     4321 aatcaactac ccactcaaaa gaatcgggac ggtttatctt atccattctg tcaagaagcc
     4381 tgtcgttcca atcatattcg gttttctttt cctcactgag ccgtgtctgt gcagagctgt
     4441 cgttatataa gtatgccgcc gaatatacaa gcgccttata gccttcggta ttctttgagc
     4501 ctatcgggag catataagta tcttgagcgc agttatactc ctgataatac gactgctcat
     4561 catcgcaacg ggggaaagga atgaaattga tctcatcatc gctccattca ttctttttct
     4621 tataaagccg ccaggtttca tcatatctcc ataaaccgtc ttgataaaac agtgtgttgc
     4681 cgtttgccca ttcggaatag cttggcgacc agttattttc ttcttctttg ggatatcgta
     4741 gatttttctg agatgtggca aactgacgca aaaaatccat tacggagatt atcttctcat
     4801 catcggcatt gcttttaaga agaccgtctt ctataccgat aattcccttt ccgatagtgg
     4861 caagaatgcc gcgttcaaca aaatatccgt caacgccgta tttcttttct tcgctgtcgc
     4921 tgaagctttc gcacatactg aggaatttgc tccacgtcca ctcgttcttt tcgtaaagct
     4981 cccacgggtc ttcaagtcct gactctttta taacactgct tctgtaccac aaaagcgagt
     5041 gcggagtact gctgaatatc ggcacataat cgtttccgat atatttcaac gattgctgta
     5101 tcttgctgta tggagcaaat tcttcagttt cgaagtctat gatatcgcct ataggctgaa
     5161 aaagcttatc tgctccgaca gagcaaggca ttcttgatat ctcagccggg aaagcatcgg
     5221 gagaatctcc tgattttatc aatttttcaa gcgtataata tctgtcaaca taactgctca
     5281 gataaacatc tataaacggt tctgtatcgt taccggagtt gtctttttca taaccctccg
     5341 gatttttcgg gggcacaccg aaaagctctt tgtatgcgcc tgcaacatct ccgttattaa
     5401 ggtcatacca gccgagaaat ttcacctttg tgtcgacctt gatatcc
//
LOCUS       DAWAOG010000064         5421 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1985_length_5421_cov_2.440738, whole genome shotgun sequence.
ACCESSION   DAWAOG010000064 DAWAOG010000000
VERSION     DAWAOG010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1985_length_5421_cov_2.440738"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1179 a         1356 c         1102 g         1784 t
ORIGIN      
        1 agccttaccc gctatcttaa cgcctttcat tattgacggg tttgtccttg cggtaacagt
       61 agcactgtaa ttgccgtaaa gcgcagtctt accactcatc ttgtaagccc ttactctgaa
      121 cttgtacacg cttgaagcct taagtcctgc ttttctgaaa gtagtcgtgc tgttgtttgt
      181 aattttgcca acacgaaccc acttgttgcc ctgatacatt tcaatgatat atccgtcagc
      241 cgaagcattc ttagtccagt ttattctcag cgcgtctgcg gctctgccgc cgagctttgc
      301 gtcctttata acagacggat ttgtccttgc ggtaacagta gtgctgtagg cgctgtagac
      361 cgcagttgtg ccgcacatct tgtacgcctt aactctgaac ttgtaaacgg ttgaagcact
      421 gagtcctgtt tttctgaatg tcgtatcgaa attatctgtt atcttgccga cacgcgccca
      481 cttgccgtcc ctatacattt caattatgta gccatcggct gagctgttct gatcccagct
      541 tatacatagt gcgttggtaa ctctttcggt gagttttacg cctgccaatt tggcaagtgt
      601 caacttatcg gtgtaattat ccatgtagtt ttcaccgcag actgagcact tgtgaagtgt
      661 gtatcccttt gtggtgtagg taggctttac aacagtgtta atgtatatgt gagccgcttt
      721 tttgacagaa gcagtttcaa tctttccgca ggttgagcaa actcttgtct gagtgccgac
      781 tgtcgtgcag gttgcttgca cagtaatcgt ccaatcactg aaattatgat tttcacattt
      841 aagcagttca taatcaaaac ctttgtcatt ggcatacttt tcgccgactg tatccgagta
      901 acagcaaatt ttaaatccat caactttagt cgtttcaaag ttaccggaaa catattcttc
      961 atgataacct aatgcatttt cgccgaaata tgttacactt gcagggataa cagctgtttt
     1021 gagtgccgaa cagttaaaga atgccttatc gtcgatatat gtgacgctat ctggtatcgt
     1081 aatgcttgta agacttgagc aattatagaa agtgccatac ctaatatccg tcacgccatc
     1141 aggtatcgtt acatcggtaa ggctttcgca tccgctaaaa gccgaaccac caatcatcgc
     1201 cacactgtcc ggtatagtaa tatttgtaag gtttgtgcaa ctctcaaaag caaaagggta
     1261 tataatccat acgccgtccg gtatagtaat acttgtaaga cttgtgcagc cttcgaaaac
     1321 tgagctgtct ataatttcca agccgtcagg aatttttata tctgtaagac tctgacaacc
     1381 gtcaaaggcg ctctcaccta tacttgtcac actgtccggc attgctatac ttgtcaggag
     1441 gtagcagtct ctgaaagcca tacttcctat cgttgtaaca ccgtccggta tcgttacgct
     1501 tgacagcata tgatagctga aagctgaact ccctatcttt gtcacactca ttccgtcaat
     1561 cttcttcggg atatcaacat tttttgcact accgttataa tcggtaatct ccaccgtacc
     1621 gtcatcaagc acgtcatact caaaatcccc atacgtctcc gctcccgccg caatagcgca
     1681 cactactgcc gtaaccgcga aagctgcaat agccaaaagc cactttttca atttcatatt
     1741 acactctcct cgtattttaa tttcctgcgc aagcacagtt ataatttaat gatatactaa
     1801 tatctttatt tgtcaaccct tttttgtaaa aatcagcacc tttttcaaaa agcattgagc
     1861 ttaccgcaaa agtacgcgtc aaatgaaaag cgttgttctc ttgcctgtgc tgtctgaatt
     1921 taaaatccac gctgaaaaca acttgcgatc attagttaaa aacagccgct cctgtcaaat
     1981 cgcagaagcg gctgttttat attcccgtca cttgatcccg aagctgtctt tgagcaccct
     2041 cttaccgctt tcggttacaa atatcctgtc atacgcgttt ttcaccgcgt tcatcgatgc
     2101 tccgtcctcg tataggttga ctatgaaatc cgcctcgata agtatctgca gatccttgct
     2161 gtcaatatcg gtgtatgtgt gatggcgagc tataagatac atgacgcgct cttttacatc
     2221 gtcatagccg ccgacagctt ccattattcg ctttgcaata tccggcccta acttctgttg
     2281 tatttcgccg tcattcctgc cgtacagtct ttcgccctca tgtataccta tatcatgaag
     2341 tatcgccgcc gcttcaagcg ttttcatagc atcggcagac agaccctcgc agactccgat
     2401 gagccgggca aagctgtgca cttttataaa gtgctgtatt cttttcgggt cgccgcggtc
     2461 atattctata gccgcattta tcagcttttc gtgcatttct attcgccctt gctctctgcc
     2521 gtactttcag tcttgctttc ggtcttgctt tcggtcttgc tttcggtctt gctttcggtt
     2581 ttgctttcgg ttttactttc ggtcttgctc tcggtatcgg tgtccttttt ctcgactgtt
     2641 ttcagcgtag tgtcggatat cagctttacc ttgtccgcgc ttgcggttat aatgcggttg
     2701 tcccacattg tgagaaggta aggcttgtcg ccgcttgata tctcacagtc gagcgtttcg
     2761 tatttttcat catacagtgt tatgtcaccg agtgacgtaa gcttattgtt cgcatagctg
     2821 tagaatttgc atctgcttac ctgtgatatg ccgtcaaaat atactatcgg tatgccgatt
     2881 atcagctttc ctccgctctc gccgtatgca agtccggtta tatcatcgca gacaggactg
     2941 gtaagatatt tgttccagtc acccggagta ctgctctctg ctgtaattgt cacctcggct
     3001 tttttgtcgc cgccgaccgc aagtactatt cctgtcctgc tgccgtcctt gtcggttttg
     3061 agtgttacct ctgcggatat ctcatcattg agttttatgc tgtttgtgct ttcgggttct
     3121 tttacttatc catcatatta agatatgtga gcataactcc ggttgtaatc agcacgttgt
     3181 tgtttgtact tccgtctgaa ccgtccgttt gcattgctcc tacagccgcg aagccttctg
     3241 tcagcgcatt gataagatgt ggatttataa atgtatcgtc caatcccgaa gcggctgttc
     3301 tcagcgtatc tccaaaattc aaatagctga gcgttgtgta atatctgccg tcaaatatcc
     3361 ggctgtccgt gacatacata ccgttttgcg aaaccgcaca gcctgccgca tcgcctacaa
     3421 gtacgcttcg tacgatcaca tcgtttgttc cgcttgcatc tattgcggta gtcgtatagt
     3481 atgaagctgt attcactttt gaaggcagtt gtattctgtt catcggtata acggtagttt
     3541 cttttccgtg tttaaatgac ggaatgaaag aggtcagctt gtccgcagat gctttcatta
     3601 tgtcaaagtg atcataggtg acgacataag ctgaattacc gttcgtataa taatcgacaa
     3661 attctcctga aagtatatat tcgtcgactg cttttccgtt tatcataccg ctttcgtcat
     3721 atgtgcggat cacggtgccg ctaccgcttt tattgccgcc gataaccgta aagtattcac
     3781 cgcactttat tattcctgca ttttcaagcc ctgtatcaaa gctgaaattg ccgcttatct
     3841 tgccatcggc tacttttgct ccgagtatct ccgaaccatt aactgccgct acaaacgtat
     3901 ttccgtacac gtttttcggg agagatgaac gcttctgctc cgccgcaact aagctgtcgg
     3961 gcagggtata ggttattttg tcttttgccg cctgtgcctg ctcggtcaga gaggcgtagt
     4021 cggtaatgtt ttgctcgagc ttttcgggaa ctttgtaaat aagatcctgg ataattccgt
     4081 ttgagcggag aatattcagt gacagaccgc ccaatatgag cactgcggca ccgataaacg
     4141 ccgcaataag gcaacctttg ctgcgcttta tccgtatgca gtcatgctca tcatcgctga
     4201 catcatagct ttcgtcatca tcgttcataa gttcaccgat agctgaacct atatcctttg
     4261 cgttgacggc tatcctttcg tcatcggaaa tgacgccgtc aaagtcatcg acctcttcgg
     4321 tagcctgtat cttttcctcc tgcggaggtt cgggcaatga aaattcctgt gttatgctgt
     4381 tttcggtatt attttcgatt tcggcgttgt ctgcttcatc agcgttgctt ccggcagttt
     4441 cgttatcgcc gacagcttca ctctctgcga caatttccga tgaattatca ttactatctg
     4501 tataatcatc actgttttct acatttccga catagttcac cttatccagt tgttcctgta
     4561 tccgtgagat catagccttg gcggcttcgg ttctttcatc ggtcaggtcg ttttctgtgt
     4621 tttcggcggt atcaatcgca ttttcggcag tttccgacgc ttctgcgaca gatggaacat
     4681 tttcggcaac gccgttatca gccggctttt cttcctcggc tttttgcttg ctttttctgt
     4741 tggtcagctc ctcggtcatt ctgcgcagag tttcttcttc tcttcttttt ctctgctcgg
     4801 taagctcctt tcgctgtgtg gctaccgcaa ttatcatacg gtagtcatcg cctgtcagca
     4861 cagagttatc tagtatctgc aaaagctcat caaacttcag atgcgggttt tcctctattc
     4921 ggcgaaattc catattgcct atcttgctgt ttccgagcag ctccagaaaa tccgagccgc
     4981 ttattttaag ttccttgagc tttcgcagga atacgcctgc ggtcatatct ccgttgtctt
     5041 caagcagttt tcttatctct tccgggttca ccttgtcggg gatctcaacg gaagaaacgt
     5101 atgcttttat cttttgttcc agactatcca ttattccctc cgtcagatgc tctgttcaga
     5161 caggaggcga gccttcatag ttacctctgc attggtaagg cacacgccaa ctgtttcttc
     5221 tgtgtatccg catagtttcg cggttttttc tgcactgcag ccgcatactg tgctgacgct
     5281 gagtacaagg cgctctatat cgggcagttc gtccagtgcc tgtcttatct ctgtgccgcc
     5341 gtctgccttt gtgataagct caccgccgga tttatttaga tgccttatct ttgtgcagag
     5401 tatcttgacc agccactcta t
//
LOCUS       DAWAOG010000065         5413 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1989_length_5413_cov_2.416387, whole genome shotgun sequence.
ACCESSION   DAWAOG010000065 DAWAOG010000000
VERSION     DAWAOG010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_5413_cov_2.416387"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1363 a         1224 c         1250 g         1576 t
ORIGIN      
        1 ctttcattgc tgctctcggt aacgtcagag ctttcgggtg cagatactgt atccgatacc
       61 gttgtctgtt gcgacgaagg ttctgaaggc ttgctctcct gagtggttgg tgacgtgtct
      121 ggggtcgtgt tgtccgaaga tgttgtatct gagccgcatc cgctcattaa ggcggctgta
      181 gtgacagcca aagccataat cagcagagtg actaagcttt ttttcatttt ttacctccgt
      241 gcgacagcct gtgactgtcg tttgagatgt acaatcttat attttcttgc acaataatag
      301 tatatcccat cgtccacgaa aagtcaagcg aatttttacc attttatgct gttaaacagt
      361 aaaatagagt gtatctttgc tgaaagattg tggtgatagt gtgaaaaagg ggaagaagcc
      421 ccgcatgggc ggggcttcag tcataattac ttattgttgc cgttcttttt cttcttgctt
      481 acaacagcgg tgataacgcc gactgcaact acaaccacaa gagctactac gcagataatg
      541 atagcggtca tatcgaatgt aacgcctgtg tttgtgttac cgctgcttgc gcctactgtt
      601 gtttcggcaa agcaggtaat gccgagtaat attgacattg ctgcggctgt taccgcagaa
      661 gtgatctttt tcatcataaa atatcattcc tttctgtaag gactaatata ctacatttcc
      721 gctgaaaaag caagtggaaa tgtatacaaa gttattgcac aatatgccct gttattccgt
      781 aggtgcggca ggagttgctg tagcctttgc tccgtccatt atgcccttga ttatctcggg
      841 ctgtgttact gtgcactttg tgcggtcaaa cagaccgaag ccatacttgc cgttccaacc
      901 gttatcccag taaacggtca cgatgtttct cttgtgagcg gcatagttga catactcagc
      961 aaaataagca cggtatgtag tgttgcgctc gtccttgtgc gtcttgtcga tagaaccgta
     1021 ttcgccgata attacaccgt agccgttttt gatatacgca ttatagagct tgtcgaactg
     1081 cctgtcaacg agcgactcgc tctgtctggg ttttacctga cccttgaggt tatcgcccca
     1141 tctgaatatt atatccttac tttcatcgcc gcagaaatcc cacggtgcgt agtaatgaac
     1201 ggaaatcata actctgcctg tgctgtcatt cgggataaca aagttgcctg cgagggcata
     1261 gccgacatcg cctgtcgtat agtcgatatc tgtgttccag cccggtacga gaagataacg
     1321 tgagccgttg tttccgccgg catttcttac cgtatcaacg aaaatctgat tataatcgtt
     1381 tatgttgcca tagttttctg ctgtaggtcc gccggaatta ccgtcaaaga cttcgttcat
     1441 cgattcgtat acaagatgct cgtcataatt cttgaaacga tctgctatct gagcccatac
     1501 ctttttgaat ttttccttga tcactttctg atcggcttcg ggctcgttgc aaagaagcca
     1561 gctgccgcct accgaataat aaccgtcgcc gtggacattt atgatcgcgt aaagaccgtt
     1621 gtttacgcac atatcgacta cttcctgtac tctgtcgagc catgcggagt cgatcgtgta
     1681 atccggtccg tcacctatct tggaaagata tgaaacgggg atacggacag acttgaagcc
     1741 ctgagccttt acttccttta taagcttttc ggttatagta gggttgcccc atgccgtttc
     1801 gctcggagta ccgcctgaat ttgcttcaag ctggttgccg agattccagc cgggcataag
     1861 gttctgtgaa aattctaccg atgcgccgcc tgtcgtgacc tcaggagcgg gagcgggagc
     1921 ttctgtttca gcttcggttg tggtggctac agtggtttcg ggtgcggggg ttgtagcttc
     1981 tgttgtttcc ggagcggggg ttgtagcctc tgttgtggtt gctgcagtag ttgtttctgt
     2041 agttgttgcg gcggttgtcg tggttgtcgt ggtttctgcc gcggtcgtag ttgtggtggt
     2101 cgaagctgtt gttgttgccg agctgctgtc aacggacgaa tttgctccgc ccgagcaagc
     2161 agagataccg gtaaccacca ttataccgct gataagagcg gcgagtattc ttttcttcat
     2221 aagtaccttt cttttctgac ggcttatgcc gtttttgttg ttttaatcat attgtaatgg
     2281 ttttcacgga aaaagtcaat agcgtaaaag gactattcaa aaattttact aaaacgatta
     2341 cataagttgc cgttcgcaac aaaaaccgcc tttcggaaac agcgtccgaa aggcggtata
     2401 ctatttgata tataaagaga ttattcctga gggtttgttg aatctccggg agtcggctct
     2461 gtgttgctgt agtcggggta ataatcgtaa ctgttgctcg tgtcgatggc gctgaagtca
     2521 ttttctttaa aagtgtcttc cgtttctacg agataaacac catcgagcgc agtcagacgc
     2581 ttaaggaagt ttccggggca ataaaccttg actgccttta tatccttttt attttcatca
     2641 aaaagtgaaa cgatattgct gcttccgctt attgcagtac cgtccgagag atagacctcg
     2701 gtcacacgga tattaccctt gccgtctgcc tttgcaagta tattctgcat atcggtggga
     2761 gtttcgttct ccttgaatgt aacataaagg aaaacatctt ccgtattttc atcagctttg
     2821 ttgtatgctt ccattgccat tttgaaacgc tcttctgcgc ttatagatgt tgtgggagaa
     2881 acgacaacgg gacttcccgt gttcagaagt gcaacagtgc cgaaagttat ggcgatcgcc
     2941 gccgcggtac ttgccgccga gatccacacc gccttgagtc tgcttgtttt tttgcttacc
     3001 gacttaccca tagcggcttt tttgattttt tcatggtcaa aggaatactc gctcataagc
     3061 tctttgtaga actcattcgt attcatttga aagccccctt ccgtaagtca cagcttaaat
     3121 tttttctttt gtcgttctat tgtgcggtaa agcttgttct tgactgtcga aagcttgacg
     3181 tcaagagcct ttgcgacatc ttcaagcttc atatcgcacg caaaatgaag atagaatatc
     3241 tgacctgtca gctcatcggt cttcattatg tcatcgaata tctgttttgc aactatcttg
     3301 ttgcttataa cgtcctccag ccttggctga ggcttggaca tctcgctctc tatcgccacc
     3361 atctcttctt cggtgtagtc ggaaaaggac gaaaccttgt cgtttttctt tttacccgag
     3421 aagtatgtgc ggcactcgaa ctttgcaatg ttgataagga aagcctcggg gctttcgata
     3481 tcctcgtgac ccttctcgct gatacgcttg aagaagcggg tatatacatt ctgaataata
     3541 tcttccgcat cgtccatatt tccgcatttg ctgactacga accttgaaac cgatttgcag
     3601 gtttcattat atacacggtt gaaatattct tcgtgaccgc tgttgcccat tggctcacct
     3661 cgcttacgtc acctactttg tagtaggcgg aatttcagaa aaagtttcac tcacaaagaa
     3721 aatattgtgt atcggataag taaaatcttt cttatggttt ttgtgcagta tcacaacatc
     3781 gaatatcatc ggatatctga cggaaattcg gctgtgttta agtgttaaag ccgaccacag
     3841 caagttttac tattatattg tacattatcg ggggctgtta gtcaagaaaa acagtttaaa
     3901 tgtgtgcaaa tgcgctttaa ggacacagta aatattgaac gaaaaatgtg actgtattaa
     3961 ctgcatcgtt attgctttgg caaaaaccaa caaataacca tacaaatttt gtgcaatttg
     4021 ccaattgcaa agtattccga aatatagtag cattaaaaat agctaaaagc taaaaaattt
     4081 ttatggagga caaaaccaat gaagtttaca agaactttag caagtgttgc tgcagctgca
     4141 gttgcagttt cttcgcttgc agtaagcgca ttcgcagctt gggacggtgc tgacaacaac
     4201 ggtaacctcg tacttaacgt agctgacatc cttcccgaag gcgtagacat caacgatgta
     4261 tacggtgtaa gaatcaactt tacagacgca tcagctgatg tattagcaca gggtgcaggc
     4321 ggcggattta tcttcagcac agcatctaac aactggaatc agctccagtg gtgcaacggc
     4381 tgtgacacaa gtgagaagcc cgaagcacac aacattctgt gggatactaa aacaaattca
     4441 atcacaagaa tggaagaaaa gcctttcttc acagctgagg atatctcagg tgctgtcgac
     4501 acatacggtc agatcgctct ttcagagtgg tggggcggcg atatcgctta ctcaagcatc
     4561 gacttcctca acaaggacgg tgtagtactg cccattaaag agcccacacc cgttgaacct
     4621 gcagatcctg tagatcccgc agatccttca gatcctgtag cacccggtac atcagaagat
     4681 gttgaagaaa cagttgacat tgctatcaag aacgcaggta agactgttat cgacgaaggt
     4741 aaagttcgta caaacatcat caacgcatgg gcaggcgacg acgcttgcgt gatcgctgac
     4801 aaggctgatt ttgcaaatgc agttaaggtc aaggttaagt tcacagtttc tgactttaca
     4861 acacctttca aggcttggct ctcattcgct aacaacgatt ggtcagttca gtactggggt
     4921 gaaggtaaca aaggtaacct cagcgcaaac ggtactacag ttgaagtaac aggcaacggt
     4981 acatacgaag ttgaactgaa ttttgacagc aagatcgcag ctgctgagtt catcgcagtt
     5041 tgcacagacc ttgaagcagt tgaaggcgat gctattccta caatagctat cgacagtgtt
     5101 aagacagttg ttgataacac accttcaact cctgatgagc ccgcaaagcc cggcgattca
     5161 gataagcccg ttgagcccgg caaccctgac aagcccgcta agcccggcga ttcagataag
     5221 cccgttgagc ctgcaaagcc cggcgattca gataagcccg ttgagcccgc aaagcccggc
     5281 gattcagata agcccgttga gcccgcaaag cccgctgatg ataacaagaa gccttcaaat
     5341 gcagatacag gtgttgcagt agcagttgtt cccgcagcag ttgcagcagc agcagttgca
     5401 gcagcaggta tcg
//
LOCUS       DAWAOG010000066         5397 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1995_length_5397_cov_2.552789, whole genome shotgun sequence.
ACCESSION   DAWAOG010000066 DAWAOG010000000
VERSION     DAWAOG010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1995_length_5397_cov_2.552789"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    417..426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1251..1260
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1657 a         1243 c         1336 g         1141 t
ORIGIN      
        1 caccagtaag atttacctcc gagcttgacg ttatctcaaa ggacggatac gatacttata
       61 ttgagctcgg tcccggcaag gtactcacag gacttgtaaa gaaaacgctt gacggtgtta
      121 ccgccgtaaa cgtagaaaat cccgacacgc ttgaaaaggc ggcggcactc tgatgctgtt
      181 tgaaagcata acggcactcg acctgtcgat actcgatttt atccataaca cactttcaga
      241 tcccgttatg gatcttataa tgacctgcct tacctacagc atagagtacg gtgcaatggc
      301 tattcttgtg ttcatcgtta tgatgtgcat aaaaaatatg cgcaagacag gctttgcggt
      361 catgggcgca acactctcgg tactgctgtt cggtgagctg ataataaagc accttgnnnn
      421 nnnnnnatgt acctctatgt gcactatccg tccgacatta tcggaggaat agcgctcggc
      481 ataggctgcg gcataggttc ggcggcactg gtaaagctta tctgtaaaaa gatcgataag
      541 cataaagagc ttaaagcaga acaaaaggag caaatacaat gacagaaaac aaagtaaata
      601 cgttcggtat actgggacac cctctcggtc ataccctctc cccccagata cacacaaggc
      661 tttttgagct atcgggcgag caggctgagt ataagattct cgacacgccc cccgaaaagc
      721 tgacagacag ctttgattac ttcaaaagtc ttgcaggctt caacattaca attccctaca
      781 agatagatat aataaagatg tgcgacaagc ttgatgacac cgcaaagcgc tacaacagcg
      841 taaactgcat agctaacgta aacggcgttt cgacaggcta caacaccgac tgcacaggct
      901 ttacaaaggc tgtcggcgca atgggtatga cccttacaga caaggttctg cttctcggct
      961 gcggcggcgt aggcagaatg atcgctatcg aagctgtccg tgagggcggg gagctgacca
     1021 ttgccgtaag agaagccgat gttcccgttg caaaggcttt atgtgaagag ataaaacaga
     1081 cctacaagga cgcaaaggta agccactgcc ttctggcaga cgtaaaggga gattttgacc
     1141 ttatggtaaa cgctactccc gtaggtatgt accccaatat tgacgcaagc ccgcttacac
     1201 agcaggctct tgacaacata acccttaacg gcttctttga cgctatctac nnnnnnnnnn
     1261 aacaaagctt atgaaggcag tttccgacaa gggtgtaact aaggtcagcg gcggtatgca
     1321 gatgcttgta tggcaggcgg ctgtggcgca cactatatgg agcggcgcag aatacaccgc
     1381 agagcaggta caggctatct ccgatgagat gatgaggctt gtgtaatgag acctgtatat
     1441 ctctgcggct ttatgggctg cggcaaaagc catataggaa gaatgctggc aagagcttgc
     1501 acagccgtgt tcatcgacct tgacaggtac atagtcaata atcagaaaat gactatcccg
     1561 gaaatattcg ctcagaaggg cgagccgtat ttcagagatc ttgaagcccg atatataaag
     1621 aacttcaagg gcaggacgat aattgccaca ggcggcggag cgatcctccg tgacgaaacg
     1681 gcagagtttg caagagaaaa gggaatagtg gtattcctta atgcaggctt cgacacctgt
     1741 tacggtcgca taaagagcga caccaaccgc ccccttgtag tgaacaacac gaaagaacag
     1801 ctgaaagcta tctatgatac aagacttccg atatacagaa agaacagtac ctatgaggta
     1861 aacgcaaata catcggataa aacgatcgca aacgaaatta taaaactcat agagcttgac
     1921 acaaaggcgc ataacaagct gaaaggataa atatgtacat ttataacgca actgtcatca
     1981 caatggcgga gaaaaaccac gaaaacggat ttatccttat agaaaacgat aaaataaagg
     2041 ctgtcggcga tatgtcacag ttaaatgact tttcaccgtc cgacagcgat atagacgcaa
     2101 aaggcaaaac agtctatccc ggctttatag atgcccactc gcatataggc gcgtgggagg
     2161 acgggcttgc attcgagggc gatgacggaa acgaggacac cgacccatcg accccgaatc
     2221 ttcgtgcgac agatatgata aatccgctcg accactgctt tgaagaagcg gcgcaggcag
     2281 gcgttacctc ggtagtatgc ggaatgggaa gtgcaaatcc cataggcggc acattccttg
     2341 ccatgaagac cgcaggctca aaaagaattg ataaacggat aataaaagat cccgttgcgg
     2401 taaaattcgc actcggcgaa aacccgaaaa acgtatataa ggataaggat accgctcccg
     2461 ttacccgtat ggctaccgcc gcaataatcc gcgaacagct caacaaggca aagcgctatc
     2521 ttgatgatat gatagactat gaaagctcgg taggtacgga cgatgagggc gaccgccccg
     2581 agtatgacgc aaagtgcgta gcgctcatgc cgcttttaaa gcacgagata cccgcacact
     2641 tccacgccca cagagcagac gacatattca cggcgataag acttgccaag gagtttgaca
     2701 tagattatgt cattatccac gcgacagagg gacaccttat agcagatgag cttcttgagg
     2761 acaaagcacg cacgatagta ggacctataa tcagcgagcg ctgtaagccc gaacttcgta
     2821 atcatacgat agcaacggca ggggtactga ataaggcagg cgtaccgatc tgcatctgta
     2881 ccgaccaccc cgttgtaccc gagcagtatc ttccgctttc ggcagcactt gctgtaagag
     2941 gcggtcttga cagggaagaa gcgctgaaag caataacgat ataccccgca cagatagcag
     3001 gcatagccga cagagtcggc agcatcgagc cgggcaagga tgccgacata gttatctttt
     3061 cgggcgatcc gctgtcaata tgcgatacgc ccgacacggt gatcgtaaac ggcaaagtaa
     3121 tataaacgga ggaaatatat aatgagtgat atccgcgaga taagcgttga gcttgttaaa
     3181 aagacagttg aagagctttg cgtaaaggca aacctttatc tgcccgatgg tatgaaagag
     3241 aagataaatt catgtatcgg ctgtgagaaa agcccgctgt caaagcaggt gctcggcgat
     3301 atcgtaagga atatcgattg cgcaaaagag cttgaagtgc cgatatgtca ggacacggga
     3361 atggcaataa tattccttga ggtcggacag gacgtgcatt tcacaggcgg agatctcaca
     3421 gacgcgataa acgagggcgt tgcaagcgga tatgtaaacg gcaagctccg cttatcggca
     3481 gtcgaagatc cgctggagcg caaaaacaca aacaacaaca cccccgcaat agtacataca
     3541 actatcgtac ccggcgacaa ggtacatatc atggtagctc ccaagggctt cggcagcgag
     3601 aatatgagtg cgcttaaaat gttcaccccc tccgcgacaa aagacgatat agtgaacttt
     3661 gttgtcgata cggtatcaaa ggcaggaagc aacccgtgtc cgcctataac ggtgggtgtc
     3721 ggcataggcg gcgactttga gctgtccgcc atacttgcga aaaaggcgct ttgcagggat
     3781 ctgaaagtca gaaatccgaa accgctttac gccgatatgg aaagcgagat gctcgaaagg
     3841 ataaacaagc tcggaatagg ccctcagggc ttcggcggta cagtgacggc gctgtatgtc
     3901 aatatagaac agcacccgac acatatcgca ggacttcccg ttgcggtaaa tataggctgt
     3961 cacgtcacac gtcatgccga ggctgagctg taatgggcaa gggtaaggtc ataggcactg
     4021 ttacggcggc ggtggcagta cctgcggctg tggttatcgg tgctatatac atactgccga
     4081 tgctcggcat atcgtcaagc aacacgctgt attacttcgg caatgacacg tcaatttccg
     4141 cagacggcac gatatcgctc ggcaaggaag atgtactcgg ctacagctca aaatattccg
     4201 atacaatgtc gggatatttc aaatcacagc ttgacgagaa cgaaacgtat atctacaatg
     4261 cgtttatgta cgcaagcgaa aacggctaca gcgatatatt cctgccagaa gatgtattcg
     4321 acggcagtta tgacaggtta caggagcttg aatatgttat aaccttttta agctgtgaca
     4381 gtccgtttgt tgcacataat tacacaacaa acagcaagct gaccggaaac gttgaacaat
     4441 tcgcaggaaa aagctatcat catatacagc ttgaaacgct cggaatggaa tatacatccc
     4501 gccgtgaagc cgcctatgaa aaggcaaaat cggtagtagc gtcaattccg caggagtgta
     4561 atacagaaag aaagaaagca cagtatcttt ataattacgt tacggaaaac tcggtatata
     4621 tgaccgacgg ctacagcaca agaaatgttc cgatagccga tctgcttatt gacggcaagg
     4681 cgatatgtga cggatacgcg gatactgtca ctatgctctt caatatggca gggatagaca
     4741 ccgcatcggc aaacggcata aataacagca aaaacgaggg acacaccgta aactttgcaa
     4801 agctggacgg cgaatactac tactttgacg caagtgcaga cagcataata aacgaaaaag
     4861 gcttcaagcc cgctttctat ttcggtatgt cgtgggagga ggcttccgac agcttcacct
     4921 ttgccgccga atttgcaaac agatgcccga aagccgaaaa aagtctgctt tccgacagca
     4981 tagacatagt tataagcgac aattccgacg caacagtcga aaatatggca gagcttttga
     5041 aatcgggcgg agatacaggt ataatcacaa ggtttgacaa aagcatcacc gatgaagaac
     5101 gtaaaagtat aacaagcgat acagcggaaa aggtcggaga acagcttgtg tctttatccg
     5161 cggcaaacgg gctgtacgga ataaaaatct ataaggaatg atttttcgat ggaacttaat
     5221 attgtactcg ttgagccgca gataccgcag aacacgggca acatcgcccg cacctgcgcg
     5281 gtgacaggcg caagactgca tatagtaaag cctatgggct ttcagataga tgataaaaag
     5341 ctgaagcacg caggtcttga ctactggcat tatctcgaca taacctatta tgacggg
//
LOCUS       DAWAOG010000067         5396 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_1998_length_5396_cov_2.514510, whole genome shotgun sequence.
ACCESSION   DAWAOG010000067 DAWAOG010000000
VERSION     DAWAOG010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1998_length_5396_cov_2.514510"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2145..2154
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1282 a         1375 c         1025 g         1704 t
ORIGIN      
        1 atacccactc accgtacttg tgtccgagtg cgtttccgta ctcgaatgtt gcttcgttct
       61 tagtaccctc gcttgaaagt ccgcattctg tgcaggaagt atagtataca gccttttccg
      121 tgcaggtagc cgctgattta agatactcag gcgcctttat atgtgcatag ctgtgcggct
      181 tcgtttcgcc atactccgct ccgcatattt cgcactttgc cttattggtg caggtagcct
      241 ctcctccgga atgctcacat tcggttatcg tagccgttac aacgtccgac ttcatcgaaa
      301 ctcccgccgt tgtagcattg ctgtaggtaa tacggcagta gtacctgccc gttgtgagat
      361 tttcgagcga cagctttctg tctgtagcgc cctctatctc tgtttcgttg ccgttttcat
      421 caatgccgat ccactgataa gcaacttttg cgtcgacctc gcctgaatac cacggcactg
      481 cacttatttg aagtgacggt atatcgccgg gataatagcc attggtgtaa tatacattat
      541 tcctcggctg aattatctgc atcggacaca cacgtaccgt aaggccgctt gtcgatgacg
      601 ccgttgcctt attaagactg tatatatcta tccactcttt tgtttctctg tcctgtaccc
      661 agaagtcggg atcgatataa tcggctatca ccttatcact gaccgtagtt acgatttcgg
      721 caaacccacc tgacgaaatg gatacattac cgccgtttat agtaaggcaa tttatattgt
      781 ttgcggccat tgtaacattt ccgccgttta tggtcacatt gctgcctgta gcaagcccgt
      841 caaatgtgac atcaccgccc ctcactatga atgcgtcttg attctcaaac gctgtcaccg
      901 cacaacgtcc cgtaaccgtc agctttgcct tgccgtttat gatgatcgga cgtgagaacg
      961 ctgtacgaac tgcgtacttg agcgtgaagt tacctccggt tatgactaac tgaccgccgt
     1021 atccgttctt gttataaagc ggatataagg tacagccctc gttttcgctc ttctgagcgt
     1081 ctttcgcagc ctcgtcaaga ttgttataat acttgacatc gtcgcccttt gttatctttg
     1141 cattgaactt gctctcgcat atctcgcaat atgtcggcgt agtaatatta cctgcggttt
     1201 ccttgtccgc tgtaggatcg accacctgag aatgatcaca gtcaacaagg tctatactta
     1261 cagtgttcga ctttatttca tagccgtcac cggtagtaac tacgaagtat atctcggcaa
     1321 catcgctgtc gcctgcctta tcaattattt cgtctgccga caatttagcg tcagttacaa
     1381 gtgcgcttaa gctgctataa attatatctc cgcttacaca ggagtttata ccgtatctct
     1441 tacttacata cgagtcgttc aggttcgagc cgtgagcaag agcaaggttg aacttcagcg
     1501 tggggtttgc gttgctgttg cgatacagct ggtttatttc tgtgctaagc gttgcgctct
     1561 ttatcggcgc ttcttctaca tttaccggta tattatcgtt atccgagctg taatattctt
     1621 tctcagcacg ttctgcaatt gaaagccatg cgccgccatc ctttttataa gcataaccgt
     1681 cagccagaat agattcaaga ctgccgtttg ctatctcgat gttgtagaat ttaccgccgc
     1741 tgagttttgc gttaccgtta acgaccagat cttctataaa accgccgcct attactgcgt
     1801 caccgtcttt ataaatcatc accgtggcga tttcaccgcc gttgacggct aattttgcgt
     1861 tttcgttaac gataataggc accatcattt tgccatcgcc gtctacggta agcataccac
     1921 cgttggatac cactatagta agagaaacaa aactgtaacc cagtttttta ccgttcagtg
     1981 caagcgttac attgcccgta acggtaatgt accgtgtaag ttcgctgttt tcaatatcct
     2041 tatacagcgt tacagtagcc gtacctacct cattagccct ttcaaaagcg tcataaatat
     2101 cgctgaacag atctgtctgc tcgcttccgt cagccgtata gcttnnnnnn nnnngcttac
     2161 agttgcaaca atttcctcat tgcagtaagg acaggggttt ccgtcaagcg cataactgtc
     2221 gttttcgtcc ttatggtcgc acttttcaac aacaataaca ccggcattgc ccttgttctg
     2281 aatatcagca tatttgtaaa tctttccgcc atccgcatcg gtcaatctaa ttctgcttcc
     2341 gctcgctatt gcctttccga aagtatattc atcgaaaacg acgctttcga atctgccgct
     2401 tacgatagat atattaacgg acccattgcc atcctcgctt aataaaccgg tatcaacatc
     2461 attgatgacc atgtctccgc cttttatcgt tattgtgccg atattagcac catctacagt
     2521 aacctttccg gaatttacaa ctaaataatt caatgtggct ttactgtcaa ttaaaacatt
     2581 ggcattctcg ccagataccg ttacaccggt aaatgaaatt gaggcttttt tcagtgccgt
     2641 aaacgttata tctccgccct tgacattaaa tgcaaacggt ccgctgctgt catttttatt
     2701 taaatcaacg gtaaatctac ccttgcttat gtcaaatgtt tcattatctt taagccaact
     2761 gtagtttaac agctttaatg tacagccttc gctttcctgg gcagcgctga tagcgtcatt
     2821 ccagttgccg taatacgata acgtaccgtc agacagctct accgaagccg taagtgctct
     2881 gttgcatata ccgcatacat taccgttatc gtcacatttt acgctttcga cggggtgctg
     2941 acaggtagct actgtaagcg ttaccacatt cgacttatat tcatatccgt cataggtaaa
     3001 tatgcagtaa tattggattt ctccgtcttc tgccactgct ttatcaactt caccgctaaa
     3061 taaataatag cgattgcctg ataagtccgt tttttccttt attaccgttc cgtcaattaa
     3121 gtatccggta acatagagtg tttcattttc cgtatatgtc acatcggcag tgaaagatgc
     3181 agtctttcta ccgttacggt atattatcgg tgactcgtca ttagcggtaa ttgttgcact
     3241 ctttatgggg gcttccgcta cagttacatc agacaataaa ttcttttcac ggtcggcaat
     3301 tgaaagccat gtattgtcat ctgtgctctt ataagcatat cccttttcga gcagatctgc
     3361 cgcagttttg ccgccgccga caattatata ttcaaactcg ccgccgctga ttactgcggt
     3421 accgtcaact gttaaggctt tgattttacc gccctgaatt accgctttgc ctttgaaaac
     3481 atccaaatca tataattcgc cgtcgccttt aactgtaaat gtgccgcccc atactgttaa
     3541 tgtatattca tcttcgttgg aaacagtgag ctttttaccg ttaaggtcaa gggttatatt
     3601 acctacagac ttgatttccc gttcaagcgt acccgtaata tccctgcaca gcgttatagt
     3661 agccgtacct gcctcattag ccttgtcaaa agcgtcgcag atatcgccga acagatctgt
     3721 cttttcactg ccgtcagccg tatagcttac ggttgcaatg atctgtgaat tgcagtaata
     3781 gcaaacatag tcatcatcta ccgcaatgtt gccgtttgca tacttgtggt cgcacttttc
     3841 aacagtaatg tcagtagctt tggtcaggct ctcaatttcg ctgtattcat aaatgatacc
     3901 gctttcagtg ccgcgaagtc ttctttcgct tgcaagcgtc ttgactatgt tatagtcgct
     3961 gtcgtctgag caggttattg tatcgaagct tccgctttcg atcacaatgt tatgctccgt
     4021 cgcgtcggta gcttttttat ttatgtctaa cgaaccgaca gtaacatttc ttattgccag
     4081 atcgccgccc ttaaacacaa gcgtatcgat ttctgcaccg tctatatcag cctttccgcc
     4141 atttattgac agttctttaa tataaccctg ggcactgttt attttaagcg caccgcttgt
     4201 taatgttaca cttcccagct tgattttacc gtcaattgta accttagcgt cagttccgtt
     4261 aactgtgatt ccgcttatca gagtttctac atcggcttgt tttaccgaag aagtgaaagt
     4321 cacgtcaccg ccgttgacat cgaactgata aaataccttc ttaccgttaa agtcaacagt
     4381 aaacctgccg cctgtcacgg taatggactc agatactcct tccgacatca gctttacagt
     4441 gctgccctcg ttctcctgtg ccagctttat ggcactctct atatccgcat agcctaacga
     4501 cttgccgtca gcatctacta cctcggcttc tatcaatgca tcacatatac cgcagattac
     4561 aaaaccgtcg ttttcgtatg aaaagctatc ctcagggtgc gagcaggtcg caagtgtaag
     4621 cttaagcaca ttcgacttat attcgtatcc gttgcacttc agtacaatgt aatattcaac
     4681 ctcgccgtct ttcgccgcaa gcttaccgat ttcataacag tgtaccatat atttattgta
     4741 caaattataa tctttaatgc gattattgcc gtttacaaaa tccgaatagg ttgcacccct
     4801 tgaaccgacc gccaattcgt atgtaatatt gacgtataaa tatttctctc cgttacggta
     4861 tataacaggc gcttcttcgc ctacccacgc aatacttgca ctctttatcg gcgcttcctt
     4921 tacttctaca ttagctatat cctgcttttc acgctctgca atagtagacc agtagccggt
     4981 cttgtttaaa ctccaatagg catagccgtc cgcaagaagc tgtcttacgt ttctgccttc
     5041 gcctgaaacc tgcaagctgt agaattgacc gcctttaatt acagcgttac cgccgtcttt
     5101 aattatgaag tcagagaaag taccaccgtt gatttccgca ttgctgccgt tgcaaacggc
     5161 aacacgtttt attaagccac tgccgttaac ggtcagctta ccgctcgtta cttctatgga
     5221 ataccataca tcagtaccgg gttgtgagag ccttttaccg ttaagttcaa gcgtaacatt
     5281 gcctgtaaca gctatcgtat ctgtaatatc gtccgtgatg ttcttctgca gcgttacagt
     5341 agccgtacct gcctcattag ccttgtcaaa agcgtcataa atatcaccga acagct
//
LOCUS       DAWAOG010000068         5386 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2000_length_5386_cov_2.271431, whole genome shotgun sequence.
ACCESSION   DAWAOG010000068 DAWAOG010000000
VERSION     DAWAOG010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2000_length_5386_cov_2.271431"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1964..1973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4335..4344
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1481 a         1219 c         1107 g         1559 t
ORIGIN      
        1 gcataccagt catcaaggtt cttcggatat gtttcctcaa cagttttcat ttcttcttca
       61 gaaagctgat caagtcccaa ctcagcggct ttcttcaata tcagtttttc gtttatcaac
      121 atatcaatga tattttttct gtacgcctga gcatatgagg cataagccga atttgtttca
      181 tcaagtccgt aatttgcgat cgtaaacgca tattcggaat agaaatcctt gaatttgacc
      241 tgataatatt ccttatcctc tgccgacagc gaatcaaccg tggaccacgc cacaacatca
      301 tcatcgtttg cagtccagat tttttctgta cttgtagtag tgtcacccga tgatgtaaga
      361 atactgtcgc tgcagcccgt aaacatcatc gcacacgcga gtattgcggc tactgccgct
      421 gttcttttct tcatttctat acgatccttc cttaatttgt gcagtaggat atttgtgtaa
      481 tttgcataac atactgctgt gtctatataa gtatatcaca aatttgaaaa aaatcaaaac
      541 aatttttcaa aaaagaggat gaattttctg tgaaaatgtg ttatacttat attgaatatg
      601 atgagattgt tgtgcaacca tgcttgtaca ctctcaaaaa atctaccgga ggtacactca
      661 gttgaaaaaa ctttcagaat acataaaact tatcgcattt gccatggcgg cggtatttac
      721 actctgcgct tgttccggcg ataatgcaag ctcgtctgaa gcttcatccg tgccggaata
      781 cgttcaggac acttccgcca aggtcggata tgtctataac gaagaggttt cgcatgacaa
      841 tatgacatat atgtttgaaa aaagccgcaa ggatattgaa accgctctcg gacttgaaac
      901 ctgctacgtt gacggtgttg cggtatcaca gtttgaaaat gcagtgaagg cgcttaaaaa
      961 cgagggctgc aaaatcattg tttccgcttc ccatgttttc gcaaactccg ctctgagcta
     1021 tgcgaaaaaa gacaaggaag tatatattct gagctatgga ggtactgctt cgcttacaaa
     1081 tctgacaaca ttccgtccta agctttatca gccgtcattc atctgcggca ctgtagcggc
     1141 atggaactct tcatcgcaca agataggaat agtcgcagac gatctcatgt actgctcaaa
     1201 cggcgttata aacgcattta tacttggcat tcagcagata tacaaagagc gtgaaaccga
     1261 tgttgagatc atctatgcgg gaacaagagc gcagaccgaa aaagcggtca acacgcttga
     1321 gggaaaaggc tgcgatgtga ttttctccta tcagtcagac gattattgta tgtactattg
     1381 tgattctatc ggtatgaaat ctatcggctt tactaatgat accgcttatt ccgctcctaa
     1441 atacggactg gtcggctatt atcttaactg ggcgacatat atcacagaca ctgtaagaac
     1501 ctgcataaac gacaacttta tagccgaaga ctacataggc ggtttcagcg aagcgtttgt
     1561 caagcttacg ccgtactcag ccgcctgcaa aaaggaaacg ctgactatag ccgacacgct
     1621 ttatgattat gtcaaaaagg gcaaagcaat gatatttgag ggcgagatac gcgacaagga
     1681 cggtcttgca agagtaggtg cgggcgcaac actcaacgat atgcagatac ttgcaatgga
     1741 ttacctcgtg tacggcgtta cttatattga taacataatc gaccctgtac ctaatcccgt
     1801 aacatctgac ctgacagtca aaacagagta cgtttcataa aaaatgatat ttaaaataac
     1861 ggcatctcat atgccgttat ttttttgctt gacttttgtt ctattttgaa ctataataat
     1921 attgttcaaa atagaacaaa aaggagcttt ttatgacaat aaannnnnnn nnnagtttat
     1981 cctctcattg gagagcgagt acggctccta ccgcaaaaaa atcatgaaca gatttttcct
     2041 ttcggcggct gaaaccgaca tacttatgtt cctgtcaaac aatccacaat acgataccgg
     2101 cacggatatt tcaagaatac gcaaaatacc gaagtcgcaa gtatcgacct cggtaaaatc
     2161 actgtgtgaa aaaggctatc tctgcgccga ctataaagaa aacaaccgca aaagcataca
     2221 tcttagttta actaagagtg cattatcggt cactgactac ggaaaaacgg tgcagactga
     2281 atttgcggat ctgctgttct cgggcttttc ggaagaagaa aaatcggagt ttgacagact
     2341 gcatataaag atagccgata atatcacaag caaaaaggag ctttgaatat ggaatatgta
     2401 attactgtat tggtatgcct gtttgcagga gtcggagcgg gactcggtac aggctttgcg
     2461 ggtatgagtg cggcggctgt tataaccccg attctggtaa cctttctcgg cattgagcca
     2521 tacaccgcgg taggcatagc actcgcgtcc gatatacttg caagtgcggt ttctgcttat
     2581 acttacggca aaaacaagaa ccttgatatt aaaaacggca ttgtaatgat ggtcacggtg
     2641 cttgtattca cagttgtcgg aagctatgtt tcaagccttg tgccatcggt tgcaatgggt
     2701 aatttttcag tatttatgac ccttatcctc ggcataaagt tcatcgtaaa gccggtcatg
     2761 accacaaaag ccgatatggt tgccgtatcg gcaaagtcaa ggataatcaa gtcggttgtc
     2821 tgcggagctg ttgtcggctt tatctgtggc tttatcggag caggcggcgg catgatgatg
     2881 cttctgctgc ttacatcggc tatgggctat gagctcaaga ccgcagtcgg cacaagcgta
     2941 ttcattatga catttacggc actgacgggc tcggtatcgc actttgcgat aggaggtatg
     3001 cctgatgttt tgccgtttgt cctctgcatt atattcacgc ttatatttgc aagaatagcc
     3061 gcaaagatcg ccaacagggc gagcgcacgg acgcttaatc gcgttacagg catcatactt
     3121 gtgattttag gtgttgtcgt actgggcttt tcatttataa gctgaaagcc ggcttaccgt
     3181 tatatagcaa aagcggagcg cttctgagcg ttcacttaag ggttatcgaa aacaacgata
     3241 tcatttgagt gaccttctca agcagtaaca aaagaaacgc cacacaactg actacaatgt
     3301 cggttatgtg gcatttttgt gttgtttttg tcagataact tctatatata catttcacct
     3361 gaccggcaaa caaggttcga tccgaatcgg ctcatccttg ccataccagg tcagttccaa
     3421 tttattcgcc atcgtttacg cctccatcta cttttgcctc aggtagtttt cccgcttgtg
     3481 cagccaattt cttttcctga gatttgacgc gccgctccat ttttttgata tcttcggaag
     3541 gtggcagttc ttccggcttg attccacgct gacccagcat ttcacgcaca gaaagattat
     3601 tctgcacgtg ttcgccggtg atagcacgtt cgccctgtaa gtctttttct tccacattat
     3661 aattggtcat ttctgttgcc agattctttg ctgcaattgt cagtgttggt aaaaaatccg
     3721 ctaggggacg attatctttc acaccgaggc gtgctttcat ttccatcgtc gtgtgaccgc
     3781 caaacagagc ctgatcacct ttggaacgaa tacgaccaaa acctgcatca tcgacaccac
     3841 gctcatagat gttctgtgaa agacgcttct cggattcacg cagtctgccg cgagcctctg
     3901 tacgctcaat tagtgcgatg cgctcttcaa tcagctcctg ctttcgcgtt tgcacagcga
     3961 agtaactctg tgcaaaggca atttcttctt ttttaggatc tccgttctgt gcaatcaaat
     4021 aacaggcata acgggtaagc atatagtcct taacgggtct ttgtgcgcct ttgccggtct
     4081 tgatcatttt cgtgacctca cgaaaatgat cagaaacttc tataccaccg gtttcacagg
     4141 aatccattgc acgggagatg gctttagcaa aattttccca acgctcatag ccgagcaagg
     4201 gcatcaactc acgggcatac caaaattcaa tgttggcggt ttcgtcacta tgtataacaa
     4261 ggtcaaactg ttccttaatc tggctaactc tctttttatc cattattctt cctcctcaac
     4321 ttcaatacgc tttgnnnnnn nnnncctcca tattatccga aatattgctt tttgcatcaa
     4381 ctcggcattg gacatattct tttcaatcat caactgcaac agccttttat aacctactgc
     4441 tattctatac tctcctttaa ccgatatcat acatattata tcacagtttt gtgaaaattt
     4501 caatatattt caggcattgc tcatatttct atagcggtca ttcgtggctg cttatttttt
     4561 gtctttttct gattttccca accgaaatat ccttcatcgg agcagtcaaa ttgccaaaag
     4621 aatagaaaag tatcggcata gccggatttt ccaagctatg ccgatatttt taaaagtact
     4681 ataattccta tgtgaggcga ccttttgtcg ctccgctttg atctttattt ctttgtcttg
     4741 tgtgacactg cctgaggtgc tgaactgcat ccgtggcact gggcacagtg accgctacag
     4801 ccgcctacac agccgccgca ggtgaactta ccctttttct tgtcactcac aagcttgtga
     4861 atgatcaata tgatcactgc aagtaaaatt accgctacga ttatcgaacc tacgatagga
     4921 tccataaaga tcactccttt ttaatgaaca ataacttact ttgaaacctt aaccttaacg
     4981 ccgagcttct tactctcctt gtaaggacga acgagaaggt agataagagc ggcatggata
     5041 agcactgcaa cgataagacc gatgatatcg ccggcacctg aaagagcgcc tgtgaagagc
     5101 ttaccgaagt agtttatcat aagggcgatc acataagcga aaccgcactc ataaccaatc
     5161 gcgaaccaga accacttagc gttgttcatt tcacgtttga tagcacccat tgcggcaaag
     5221 cagggagcgc acagaaggtt gaatacgagg aatgacatac cggttactgc tgtgaagctt
     5281 gaagcgagtg tctgccatac tgtagcatcg ccgcttccgt aaaggatacc cattgtcgca
     5341 acgatatttt cctttgcaac aagacccgta acagccgcaa cagttg
//
LOCUS       DAWAOG010000069         5335 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2017_length_5335_cov_2.539205, whole genome shotgun sequence.
ACCESSION   DAWAOG010000069 DAWAOG010000000
VERSION     DAWAOG010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2017_length_5335_cov_2.539205"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    490..499
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1417..1426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3284..3293
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1362 a         1168 c         1325 g         1450 t
ORIGIN      
        1 gcgttacata tggtcgggag aatgctcagg gagatgtcat ttcgcagtgt gagaatagtg
       61 gcaggttcaa gcgaggagct ttacgccaaa gtctacaaaa aggaaaagga ttatgtagcg
      121 gtgtcgatgg tggtagttct gcgtgaatat cagggaaaag gatatatgaa aaagatactg
      181 gaacaccctt tttccgaagc ggcaagggag ggtataccct gcgtgcttga taccgacact
      241 gcacttaagg tagctaaata cactaaatgc ggaatgaaga ataccgctaa aaagcaactg
      301 aaatccggtc agtatctgta tacgatggag tacagggaag gctgacgggt tcggaaagca
      361 tattgaatac agatttttcc gtcccgcatc atcggggcgg aatttttggt tgcaagtgca
      421 ggaaatacgc ttttttacag cggttttaag ctgtaaacgc ttgacaacag agccgaaata
      481 atgtaccatn nnnnnnnnna tgctgtatta ttgccgcacc gataaccgaa gccaaaggta
      541 cgctgtggtc gctgtttccg ccggtcatag cgataggact tgcgcttatc acaaaggaag
      601 tgtattcctc gctgtttgtc ggaatattgt cgggcggaat aatctatgcg gcggcatcgg
      661 gaacgggatt tgagggaacg ttcaaggcgg tcgtgcagga cggacttata acaaatcttt
      721 caaacgcata caatgtcgga atactggtgt tccttgttgt actgggaata attgttgtac
      781 tgatgaataa ggcgggcggc agccgtgcct acggcgaatg ggcggcggct catatcaagg
      841 gcagacgggg agttgcactg tcaacgtttt tcctcggtgt tctgatattc gtctgacggt
      901 aggctcggta atgaggccga taaccgacaa gcacaacata tcacgttcaa agctcgctta
      961 tcttatagat tcgacagccg caccaatctg cattatcgca cctatctcat cgtgggcggc
     1021 ggcggtaagc ggtacggtcg agggcgtaaa cggaatatcg ctgtttataa acactatccc
     1081 gtataatctg tacgcatttt taacgatact tatggtaata ttcatatcaa taagcgatac
     1141 ggattacgga ccgatgaaaa tacacgagga caacgcaaag aacggtgata ttttcacgac
     1201 aaaaaacaat acttacgagc aggacgcaca gccggtgacc gaaagaggca gggttataga
     1261 ccttatactt ccggttgccg tgcttatcgt tttctgcgtt gtgggtatga tctatacggg
     1321 cggatttttc tcgggtacgg attttgtgac ggcatttgca aactgtgatg cggcttacgg
     1381 tctgtcactc ggctctatat cggcgctgat agttatnnnn nnnnnnatag ttataatcgc
     1441 atattatatg ctccgccgtg tgctgaagtt caacgaatgt atggattcta tcgccgcagg
     1501 atttaagcag atggtgcctg cgatacttat actgactttt gcgtggacgc ttaaaacaat
     1561 gacaaaccac cttgaagcag gtgcgtttgt ttcgggagtg gtacagggcg caacggcgct
     1621 cagcgtgctt ctgcctgtaa tactgttcgt ggtggctatc gggcttgctt ttgcaacggg
     1681 tacatcgtgg ggaacgttcg gcatacttat ccctatcgtt acgagcgtgt ttgaggcgga
     1741 gcttgcgaat gtttcgcaga cgggagaaat tccgtcaatg gtcatcatct gcatttcagc
     1801 ctgccttgcg ggtgcggtgt gcggcgacca ctgctcgcct atctcggaca ccaccattat
     1861 ggcatcgaca ggtgcgcagt gcgaccacgt caatcacgtt tcgacacagc tcccgtatgc
     1921 gctgacagtt gcggcggtgt gcgttgtcgg ctatctgctg tcgggctttg tgcataacgt
     1981 attcatagtg cttggcttct cggcggcgct tatgctggcg gtgctgtttg caataagatt
     2041 ttttgtaaag agaaaagaaa gcaggagctg agaaatggca acatcgcagg aatatatggc
     2101 aataccgcac aattaacggc tatcgccttt gccgcacgct tgcttgcata tttcccgtca
     2161 tcttgcacaa atttcgcttg acaggcgcaa gcctgcctgc actcaattta tacaatctga
     2221 cgaaaaatct gactgcgcga tcgcacgaca ataattatgc ggtattgcca tagaatttgt
     2281 ctgtgaacag cttgtcggta tcgaaaacgt gcggtataaa aagatgttcg gagaatatat
     2341 ggtttatgtc aatgataagc ctgtcctgct tgtctgcgac aacacggtat atgttaaaaa
     2401 gctccccgaa atcgaagagc ttatgtcgtg cgctgagtgc ggtgtgccgt atgacggcgc
     2461 aaaggagcat tatattcttg atatcgagaa cagggagctt acggcaaagg ctgctcaaat
     2521 tctcgagcag ataacgcctg tgcctaaaaa gaaaaataag aaaaaataat gtaaatgggt
     2581 tgtcagcata aataataacc gctgtgcgtt ggcacggcgg ttatttgcgt ttgctcttgt
     2641 ttgtcgggct ttttcatatt atttaatatt ttttatccag ccttcaagct ttgatttatc
     2701 ggtcataagc tttgcaccgt tgaagtgcag atcaagaccg ttttttacat ttgcgccatt
     2761 gcattcagcg gcgatatcct ttaatgtgtg accgagccag ccaccgtttg ttgcgaacgg
     2821 ataaattgtc tttcccgaaa ggtcatagct ttcgatgaat gtttttaccg caggcgcgaa
     2881 tgtgtaccac catacaggtg tgccgagtat tatagtgtcg tactgcgaca ggtcaacgtt
     2941 tatcggcttt atcgggggct tgtagccgtt tcttatctcg ttttgaccct gatcgacaac
     3001 ggcgttgtaa tctcccttat aaggtgtgac ggtttcaatg accgccgtgt cggcggttaa
     3061 ctcttttgct atatgcgcgg ctatatttgc ggtgtttccc gaataggaat aatatattac
     3121 gattgctttc attttacttt acctccgtat agattttctt cgaatagttg aaagctgccc
     3181 acgccttagg ccagcctgca tagaatgcaa tgtgagttat ggcctccgcc atttcttccg
     3241 cagtaattcc gttttctttt gcgtgcataa tgtggtattc aagnnnnnnn nnnagtatgc
     3301 cgcttccgat aagagcggct actgttacaa gacttctgtc acgggctgag agtttgtcct
     3361 ctcttgacca aacttcaccg aataatacgt cgtcgttaag ctcggcaaat ttcggtgcaa
     3421 attcgccgag tgcatctctt cctgctgttt gctttttcat aaatgagatt ctcctttatt
     3481 taaattacag tgcattgtat tctttgtccg aaacctgttc gcaccattcg cttgagcatt
     3541 cctcacccgg catttcaacc gctatatgag aaaaccagct gtccttcttt gcaccgtgcc
     3601 agtgctttac tccctcgggg ataacgatga ctgtaccggg tacaaggctt actgcgtcct
     3661 tgccgtattc ctgataccag ccctcgcctg cggtgcagat aagtagctga ccgccgcctt
     3721 ttttagcgtg gtggatatgc cagttattgc ggcagcccgg ctcaaatgtt acatttgcaa
     3781 ggaataatcc gtcctctgcc tttgtcaggg aatttaaaaa agaatccccg ataaaatatt
     3841 ttgcgtaagc cgtgttagcc gcgcctgtgc cgaacacgtt gagcttttca aattcttctt
     3901 ttgataatgt tttcatctgt gtttcctcct gttttttacg gttttcttta gctgtggcta
     3961 aattgtataa cttggagctt actccatgtc aataggacat agaaaacttt catcataatt
     4021 tcacaataca aattctgagc aattgtcgat attctccgcc tgccacgcat tttttcttga
     4081 ctatgcgcat tatttagtgt aaaataaaat tgtataacta aaaaacggga atgaatccgg
     4141 agggtataat ttatgctttt acatcaggta aagctgccac acttcaagca ttccgccgag
     4201 agcaaaacag taacgctccc actgcccgac aaggtagtga taagtatggc tcagcatctc
     4261 ggtgcaccgt gcaccccgac agtaaaggtc ggtgacaagg tgaagatagg cgatgtgata
     4321 ggcagcagcg aggcggctat gtccgcaccc gttcacagca gtatatcggg aacggtaacg
     4381 gcaataaccg atgtcctgca tatatcgggc agaaatatgc agtcggtagt tattgaaaat
     4441 gacggactgg aagaggtcag cgacagcgtc tgtcccccca ctgtagaaaa cagggagcag
     4501 tttttacagg cggtaagaaa cagcggctcc ataggtcttg gcggtgcagg tttcccaaca
     4561 tcgctgaagc tcggctttga ccccaaagaa aaacagcccg actttcttat tataaacggt
     4621 gcggagtgtg agccttatat cacctccgac tatcgctgct ttatggagga gggcgaggac
     4681 gttctcgagg gcgtgttgct tatgctgaaa tatctcggaa taccaaagtg cataatcgcc
     4741 attgaaaaga ataagccgaa agccatcgag aaaatgacaa ggctttgcga aaagcacgac
     4801 aacatcacag taaaggcgct taagtcaagc tatccgcagg gtgcggagaa aacgcttatt
     4861 ttcagcacga caggcagagt tgtaaaggaa ggcacactgc cccttgcaag cggctgtata
     4921 gtggtaaatt cctctacagc ggcatttatc gcacagtata taagaacagg tatgccgctt
     4981 gtaaaaagaa gaataaccgt tgacggaaat attgtgaaga agccgtcaaa cttcattgtt
     5041 cctgtcggaa tgtcgatagg cgagatagtg tattacgctg agctttccga caagcccgac
     5101 aggataatac tcggcggacc tatgatgggt gcgtgtgcat tcgaccctga ttatccgctt
     5161 tccaaaacaa acaacgcagt gcttatgttc gcagacagcc ctgtatatga gccgtctgag
     5221 tgcatacgct gcggcaagtg cgtgagagcc tgcagtatga atcttctgcc gacagagctt
     5281 gaacacgcat ttgatatgag ggatgccggc agacttgaaa agctgaaggt aaacc
//
LOCUS       DAWAOG010000070         5324 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2023_length_5324_cov_2.386980, whole genome shotgun sequence.
ACCESSION   DAWAOG010000070 DAWAOG010000000
VERSION     DAWAOG010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2023_length_5324_cov_2.386980"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1297..1306
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2562..2571
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3363..3372
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4880..4889
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1500 a         1113 c          904 g         1767 t
ORIGIN      
        1 cttgtaaaca acgggccgta cctcaggatt accaagttta ccctttgtct gaccttcaaa
       61 ctcacattca gggagcttta ccgagattac cgcatttata gcctctctta tagcctcacc
      121 gctcagcgga acaaattcct tgacctcgtc ctgcttcttc tttgactttt tattgttgtt
      181 tgcctgctgt tccttatact gctttacaaa gtcgtttatg accttgttca gtgcattctt
      241 aaaagcgatc tcatgcgtac cgccgtcaac cgtatgaatg ttgttagcaa acgatctgaa
      301 tgcttcactg ttgaagtctg tattgtactg gaacgcgatc tctactgata tattgtcaag
      361 cagaccctca atatatacaa catcgggatg aagagcctct gctcccctct tcttctgaag
      421 ccactctaca tagcttataa taccgccctc gtactgaagg acctcctgct taggcttgtc
      481 ctcatctctg aggtcgctta aaacgatttt aagtccggca ttcagaaaag cttgttctct
      541 gagtctgtca agcagtatat catatttgaa ctctgtgctg tggaagatag tgtgatcggg
      601 cttgaagcgt acctgtgtac ctgtcttatc ggtcgtacca ataaccttca tctgctcctt
      661 gtaatgaccg ccattttcaa aacgctggaa atgtatctcc ttgccgtcat ataccgtaag
      721 ctcaagccac tcggaaagcg catttacaac agacgcacca acgccgtgaa gaccgcccga
      781 taccttgtat ccgccgccgc cgaacttacc gcctgcgtga agtatagtat atacgactgt
      841 tgcggcgctt atcttttcct taggatgaat gccagtaggt ataccacgtc cgttatcggt
      901 tacttcaata acatctccag gcagtatctt aacgtttatt tcgttacaat agcccgccag
      961 cgcttcatct atagcattat ccacaatttc gtatacaagg tgatgcagac cgctttctcc
     1021 agtagaaccg atatacatac ccggtctttt tcttacagct tcaagtcctt caagtatctg
     1081 aatatcgtct gcaccataag cgtgttcgac ctgtgtatta ttctctatat cctttatttc
     1141 gccgtcaatc tcaggctcgc caacctctcc ggtattgacg tttttatcaa gaatttcttc
     1201 tgccatgctc tttcctttca tcatttccgc aatttctcac aatactatat ataattatat
     1261 cacatttaac tttcaaagtc aactttgaat taaagannnn nnnnnncaca ataatccaat
     1321 cagcagcgta aacaacagtt ccgcaatgaa ataagataga gttattcata acaaacctat
     1381 atttattcgc atttgaactt atattctcat ttgaaagcat atagaaatat tgtaaatatt
     1441 tatcatttta aaaaatcaca ataaacgtaa atgcagaaat ttatacgctt aatcaaattt
     1501 aaaatcgaaa gcgaacatac ggttttactt tatgaagcag ttaaaggtaa gtgtgtatga
     1561 agcttattac acgctgtaag cctgataaca aagccaaata cagaaaacaa gcagaataaa
     1621 ttttcattgt gttttctgta tttacaatct tatttcattt tctgcaaatc gccttttcag
     1681 cgtgaaaacc gacacatttg agatatatac atagtcatcg gtaacgatga agctctttgg
     1741 catatcgtag gaaacataat agatcttgcc ctgcttctga agcttcatca gatagtcgtt
     1801 tacatccttg tttaccgagg ttttctcaat gtcaaatata cccagtatct tgtcaaccgg
     1861 cagtgataca tcgccaccta tatgaatata catatatatc attcctccgt aaaagtgccg
     1921 ctttctacat tccagatttt accgttttca agttctttta cgtcatttat attacagcaa
     1981 gtaataaata cctgtgagtc cgtaatgtga ttcaggacga aatgttgtct gttgttgtca
     2041 agctcgctta aaacatcatc aagtatcatt atcggtgttt catctttttg tcggattatc
     2101 tctgcctcag caagctttaa agcaagcgct gtacttctct gttgtccctg actgccgaac
     2161 tccttgacat cgttgttatc gatatacagg ttcatatcgt cacgatgtac gccaagtacg
     2221 gtataacgca ttttcagctc acgctgaaag cttgcattga gtctttcaag atattcgttg
     2281 taaagtccgt ttatcgcgtc acatttaagc tcagtgctgt caaagatgct tgaataatac
     2341 cggagcttaa gctcctcgcc gcctgaaagc tcattatata gccgtgaagc tatcttttca
     2401 agctgagaaa agtacttcag cctaccataa gtaacattaa gcccttcggc ggctaaaacg
     2461 ctgttccagc tctctatctg aggacgtatt acagataaat catcgccaaa attgatacca
     2521 aacagtaaat tattcttatt ttttaacgct ttgttataac gnnnnnnnnn nagaaagctt
     2581 cttaagatgg acgggagtct gcatcatagc tacgctatcg aggtattctc gtctgccctc
     2641 gggtattcct ttgatcagat ttaggtgttc cggtataaaa acaacgtatt ttaacaatcc
     2701 gtataactct aatgcagatt ttatagcaag tccgttgtat tttatctctt tcttattatt
     2761 acatatagta aattctatca gattttcact atctgtatta aaagatgtgg taaaccacat
     2821 ctttataacg cacttttcct tagaattatc caaagaaaaa ggaatataag agctttgtct
     2881 ggaggtacgg aatgaaggtc ccatacacag agatatagcc tcgcagagat tagtcttacc
     2941 ctgtgcgttg ttgccgcaaa gtacatttac attctcatga aagcaaaact cctgctctct
     3001 tatattcctg aaattcttta tataaagccg ttttatctgc atcttacttc atacagcact
     3061 tatactgctg ctcgtcaagt tcgatagtat caccgtctac gattttcttg ccgcgcattg
     3121 tacatatttc tccgtttacc tgaacaaagc cgttctgaat ggcatttttg gcttcaccgc
     3181 ctgtttcgca agctccgctg aacttcagaa actgttcaag cttgataaaa ggaggtttta
     3241 catttacgtt tatatacatt ctaatcactc tttcttgtgt atttaagctc tgaaacgccg
     3301 ctctcatcgg gaatagtaag cgtcagagta tcaccttcaa gcttatatat catcttataa
     3361 tannnnnnnn nngggttctt atgagaagat gctgaccata ttgcaaacaa gacacacatt
     3421 ccgactatga gtaaaccaag tattacacag agtaatattc ttggtgcttt actctttttt
     3481 ctgactgagc gtccgtcact gtggcttatg ataaaatcaa caatatcaaa gtcttctttt
     3541 tgttcaatat gctgtgtacc aacatctgta ccattggtac attccggtac agtattggta
     3601 tatacgtttt cattttttgt tgcattattt acagtttcat ccataattta taccacccta
     3661 tgaagtatct caggactgtc gctgtcagac aaagatatga ttatctccga gccttttata
     3721 ctaatattat aatatttcgt ttcgttattt gattgtaatg caagtatatt atttttatat
     3781 gaatatccgt aaaattcagt atacgaattt acatttgaac taattgtaag tgtattatca
     3841 tcaaaggtat attttctaag cccgtcggaa gtttcccaag agccttttag aaaatttcct
     3901 gtcagcttcg gaataatgat tattgctata attacaattg taattattat aaaagcaata
     3961 taaatccatt ttctttgctt tttaaaaact gtcatattat cggcgtctta cagggcttac
     4021 aataaatgta aagctatcac cctcaagcgg aactatctta agtgccgtat tttgtacaaa
     4081 cagaaatttt accttatcgc agtaggtgtt tttaagtgca tcaagaatat atctgctgtt
     4141 aaatgaaatt ttgaactctt ctccgctgta gtcacacttg atcttctgat ccagcttacc
     4201 gatcgcagtt tcgcagaaaa ctctgagggt attgtcatta aaggtaaaat taacaggtga
     4261 ttttatctta tcgctgataa gtaaaagtgt tctgtctaca cattcgataa gctctcttac
     4321 atttaccaca gcttcaacat ttgaaggcat actgattatc tttgtataat cgaaaaaatc
     4381 accttcaagc agacgtgaaa aaacagtata tttttcaatg ctgaatatac cctgatttct
     4441 gtctacggaa atagttgcaa tcttgttctc atcatcgctg agaagtctta aaagctctga
     4501 taaagtcttt gcgggaacaa caaagtcaaa atcctcgtgc aacagacttt cttttcttat
     4561 tgctattctt atgccgtcta ccgatatcac attcagctca ttgttcttta tctcaaattt
     4621 tgagcccata agtacaggct ttgtatctat tgtggcaact gcgtaaagag tctgacttat
     4681 aacgcttttt aaaagcggtt cacttatctc aaagctttca cctcttgaaa gctcgggaag
     4741 aagaggatat tcttcactgc ttattcccat tatagaatag gttatatctt cgcatatcaa
     4801 attgagcttt aaaggctcat attcgtcaaa gatgatctca ccgctgggca tctttcttac
     4861 aatgtcgcaa ataagacggn nnnnnnnnng tgatacccag ttcaaggtca taaccggtca
     4921 ctgtcagcat atttcccgta agactcaagt atatgccctc aagggctgta ataaccgatt
     4981 ttgaagatgc tgcctttgct gttgtggtaa gggcatctaa aatgtcattt ttctgaacgc
     5041 tgaattttat cattggtatt cctttcattt taaggcttga tctgttttgg tctgaaaaga
     5101 cacaatgtca acaatttgtt tattatccgt gtgagtatag ttttaacatc cggttctgta
     5161 agaacagtta tcgccgcagg cgataaataa ataaaataat attaaatatt acggtggtga
     5221 cggtgggggc ggtttaatgt gtttaaagtc ggaaatttgt gataaaacct gtatgcgggg
     5281 gagtttgcgc cggttgaata tacggtgcgg taagagttaa gtgg
//
LOCUS       DAWAOG010000071         5308 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2030_length_5308_cov_2.119170, whole genome shotgun sequence.
ACCESSION   DAWAOG010000071 DAWAOG010000000
VERSION     DAWAOG010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2030_length_5308_cov_2.119170"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1171..1180
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3056..3065
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4450..4459
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1422 a         1256 c         1154 g         1446 t
ORIGIN      
        1 acgcatcact gccgctgata ttttcaagat acggtgcggt tttgcatacc ccgagatagt
       61 cctttatgaa atcacactca ccgctgtgaa tttctttgac cgtttccgcg ttctcacact
      121 cgctgtcaaa gttcatcgca ccgtttgccc cgaatcccga aaatgacggc tcgggagcac
      181 cgaaaagaag ctcaaaatag atattgtgct tcatatccgg gtggtgattt tcgtaatatc
      241 tgcggttaag cgatgacgag aagcaatacg gaataagctt tccgtcggca aaatacgttt
      301 cgctgaaatc actgtcaagc tgtactctgg tatttgtgcc cgcaagtacg cctgtcacat
      361 ttatatccat accgaatttg cggttcagaa gcgcatcaag cattatgctt ccgcttcctg
      421 cccaaccgca gtcgaccgtc aggatatttt tacgtccggc ggctttttcg tcaaaataat
      481 cctttgcact ttttatcata gcggagtagc tttcagctat gcgctgaata ttattgtaaa
      541 caatctcttc cacacgctta cggttggcat ttgtcagcat ttcatcgggc atcagcgagt
      601 aatctgtgca ggaaagctcc atagcagaga aaatctcttt gagggtatat cccctgccga
      661 ccttctgact tacaaaccgc cttacataat caaacgggaa tatatccgca cacagctttg
      721 tagcggctgt cctcgaccag tatatatact ctgtcgccac atcgggataa aggcggtcaa
      781 aaacctgctt taaaatatat ccgtcacgag caaagaaaaa tatcttatca gcctttttca
      841 ggagtgccgc tttatatata aagttgcaat atccgagtat aaggagtcct ccgcatttgt
      901 agccatactc ataagccttt gagcaatgcc cgccgcagta cagccttgca tttacaagcc
      961 ccgagtaaac cgagccgaca agatatgaca tatcgtgcgg tctgtactcc cccgcaatgc
     1021 tgttgatgtt gggatagtaa acagtttcaa agcctgcctt tgcggcattt ttaatatcgg
     1081 aatgccgatt gtcgccaata tggatataac gtttatttcc gtactgtttt tttattatat
     1141 catatatcct accgtcactt tttccacaac nnnnnnnnnn acatatacat atcggttgtg
     1201 ataatgacgg ttttccctgc cgacaatact tttttataca cttcgagcat aaacggattg
     1261 gcgcggcaaa gcgcaagctc ggtatcgcat tcggtcgtta tgccgagctg cggggatatc
     1321 cccgctttct ttcccacaag ctcatatata tcttcgattg ttatttccgt agtaccgtct
     1381 gtctgaagct tctgctttct cgctttgctt tcgcactttt tgcgcaacga acggaaatcg
     1441 ggtattccga gcttttctcc tattacatag aacaggtcgg cgggggcttc aaacggtcgt
     1501 aatatcagcg tatcaaaaac gtcaaaagat acaatatcag ccttgcaaag cacagcggcg
     1561 gtttcttctg ccgacatttt gaaagccgaa ccgctttcat ttcccgcttt ctgcttgtat
     1621 ttaagctctc tgtccggaat gtgcagaagc gtatatttta tattgagcca cacagcatat
     1681 ccccatgata taaaaggtgc ttttgcgtgc aaaccatcat gagtcatcac aaaatactca
     1741 tagtgatgct ttaccgtgcg gttacggttt ataagaagat tatacagctt tagttttgct
     1801 cccatttgaa agaactccgc ctttccttat tactctgtta ttttattata atatcataaa
     1861 acgggaagaa acacaagaac agccctcatc aagtttattg atttactctg ttcaatgtat
     1921 aaaaacagcc gcagtcagtg ccgcggctgt cgatatattc gcttgccgat atcagataat
     1981 atccctgata tcgatatttt cacctgtaag acctacataa gcgccctgct tagcctcatc
     2041 actttctgcg tgagcaagaa gccacttgtt gcaagcccag agtgtcttat cctcttcgta
     2101 tacgggagtg atgctgggct ttgcaccgtt tgttcccttg ggaagtgcaa taaccacctt
     2161 gaagcaaccg tccttagttg cattgcaggg agcgtaatgt aatgtagttg cataaacctc
     2221 gaccacatcg cctgcctttg ccttgaaagc cattacctta gatgtatcga gcttgccgtc
     2281 aactatatcg tcttcctttg ctacaagcag gataaaatcg tataagccga tgttgaactc
     2341 gctgtcacgg tgatattcaa ggcagttgag ctttgtgttg tgaccgttgc accagccgag
     2401 ctggataggc ataccgccgt aagcgttatt ctgtaaaagt ccgaacagct ttgtgttttc
     2461 aagcgccgcc tcgctcggaa catatcctac agcatccggt aacggtgtgc tcttgtcgag
     2521 cgccgaaagc agatcgcctg catcatagcc tgtatgtacc ttgccgtatg cggcaaattc
     2581 cttatcatta acagaatatg tcttcatttt taatactcct tttaaagcaa aacccggcac
     2641 tgccgtgtta acgttttcat aaaagctaaa gaaaacagcc ataagcttga aaactcatgg
     2701 ctgtccttat gttaatatta ttcctcagcg ggagcgccaa caggacatac acctgcgcaa
     2761 gcaccgcagc taacacaagt gtcagcgtcg ataacgtact tgccgtcacc ttcggaaatt
     2821 gcctcaacgg ggcactgagc agcgcaagcg ccgcaagcga tgcaatcatc agaaatttta
     2881 taagccatcg gaatgatcct ccttgtgata atttgtcata gagttatatg acttgcttat
     2941 attgtaacac actttcggaa aaaatcaagt ccttttgata aatttatatt gaaatatcgc
     3001 gcttttttct gaaattttac gtcattttca agttagcgta aagtaacctc aaaacnnnnn
     3061 nnnnnttgtc tatctgttta ccaccctgaa aagatattac gcagggtata gaatctatgc
     3121 cgaaattgac agcaagatcg cgctcctcat cggtattgac cttacagatc ttaatttccg
     3181 ggtgttcttc gccgagctcg tcaagcacgg gggcaagcat tctccacgga ccgcaccacg
     3241 gtgcccagaa gtccactaaa accggcttat cggactgtaa tacctcttcc ttgaaattct
     3301 cagctgttac ttctatatgg ctcatagctt gtacttcctt tcgttttcgt tgtttgttat
     3361 atcatcagga atatcgtgcg taattttatc gcacttattc tgattttgat tttaccatat
     3421 gtaaagggaa ttgtcaagat aatccgtaat aattactgat tttatttttt caaaaacaag
     3481 ttgcaaaagc cgccgggata tgatatactt tgaagtatgc ggaatgccat aatcggtgcg
     3541 gatttctgca ctgaatgacg gaaagacaaa aaggaaagga aactggaaaa tggcggaatt
     3601 ttcaacaaac gagcacgagc tcgtactcgt cattgacttc ggcggtcagt acaatcagct
     3661 tattgcccgc agagtcagag aacataacat atactgcgag gtcatctcgt acaaaacacc
     3721 tatagagcag ataaaagcga agagcccgaa gggtatcatc ttcaccggcg gtccgagaag
     3781 cgtatatctt gacgactcgc ccagaatgac aaacgaaata tttgaactcg gcgtaccgat
     3841 attcggtatc tgctacggcg cacagtttat ggtttacggc ctgggcggaa cgatcggcaa
     3901 ggctaacgaa aacgctgccg cagaatttgg cagaacagag tgcgaatttg acacaacctg
     3961 ccccgtattt gacggactta agaagaactc ggtcgtatgg atgagccaca acgactacat
     4021 cgaggttctc cccgagggct tcaaggctgt aggtcacagt gacaagtgtc cctatgccgc
     4081 aatcgagaac gttgaaaagg gattttacgc tacgcagttc caccccgagg taaaccacac
     4141 cgagcagggc tttgatatgc tcggaaactt cctgtacaag gtatgtcact gcaagggcga
     4201 ctggacaatg aagaactatg cggaagaagc tataaagcag atccgcgcaa aggtcggcga
     4261 cggcaaagtt ctccttgctc tttcgggcgg tgttgacagc tcggtagcct gcgctctgct
     4321 ttcaaaggca gtcggcagcc agctcacctg cgtgttcgtt gatcacggct ttatgagaaa
     4381 gaacgagggt gacgaggtcg agcaggcatt caaggactgg gacgtaaact tcatcagagt
     4441 aaatgccgcn nnnnnnnnnt ttatcggcaa gcttgagggc gtgtccgacc ccgaaaccaa
     4501 gagaaagacc atcggcgaag aatttatccg tgtattcgag gaagaggcaa agaagatcgg
     4561 caaggtcgac ttccttgtac agggtacgat ctaccccgat gttatcgaaa gcggcacagg
     4621 cgatgccgca gttatcaagt cgcaccacaa cgtaggcggt ctgcccgact atgtcgactt
     4681 caaggaaata atcgagccgc tccgtctttt attcaaggac gaggtaagaa agctcggtac
     4741 tgagctcggt ctgtcagacg tactcgtatg gcgccagccg ttccccggtc ccggtcttgc
     4801 gatcagaata atcggcgaga tcacccgcga aaagctcgca attcttcagg acgcagacta
     4861 catcttccgt gaagagatcg cgaacgcagg tttagaccgt ggtataaacc aatatttcgc
     4921 cgttctgaca aatatgcgtt ctgtaggcgt tatgggcgac gacagaactt acgactatac
     4981 tcttgcctta agaggcgtta ctacaagcga ctttatgacc gccgacttcg caagaatccc
     5041 ttacgacgtg cttgaaaaag caagcgtgag aattgtaaac gaggtcaaga atatcaatag
     5101 aatttgctat gatattacga gtaagccacc ggctactatt gagtgggagt aatacaagcc
     5161 gatgctaaga gccgccttcg ttaagatgta ggcagctctt tttgctaatc cctacaatgt
     5221 agcttatgtg ttgttgcact gagtgcttta aaaatcatct ttagatcatt ttcgggtatg
     5281 attatatcat aacatattaa ggcgtgat
//
LOCUS       DAWAOG010000072         5286 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2038_length_5286_cov_2.380424, whole genome shotgun sequence.
ACCESSION   DAWAOG010000072 DAWAOG010000000
VERSION     DAWAOG010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2038_length_5286_cov_2.380424"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4622..4631
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1447 a         1093 c         1416 g         1320 t
ORIGIN      
        1 ttgtttcctg cgttatggct ataatctata tggtgagggt aaatgcggat tataagctgt
       61 ttatccgtca gataaaggta cacagggata tggcgataag gataataaag gtaggtctgc
      121 ctacaggcat tcagaatatg gtcatctcct tttcaaacgt gcttgtacag gcaagcgtca
      181 acagcttcgg ctcggatgcg gtagcaggct tcggcgcata tcttaaggtg gacggcttca
      241 acatcctgcc ggtgcttagt ataagtatgg cggtgactac ctttgtcggt cagaactacg
      301 gtgcgaataa gcccgaacgt gttaaaaagg gtatgtggac agcactcggt atgggcgttg
      361 tttacactgt cataacaggc gtcctgctgc ttgtgtttgc cgacccgatt ataaggcttt
      421 tcacaaacga tgacgcagta gccgcctgcg gcgaaacagc aatgaaatac ttctgcccgt
      481 tctatttcct gctagcaata atgaatgtgc ttgcgggggg tgtaagaggc acgggcaaga
      541 gcattcctcc gatgctgata ttactgttcg caatgtgcat tttcagaata ttctggatat
      601 ggctcgtagt gccgcttttc ccgactattg acggcgttta tctgctgtac cctgtttcgt
      661 gggtaatagg cgttgtgctt atggcactgt atacctggaa gggtaaatgg atgccgaaaa
      721 tcgagtaaat atccgacttg aaatgaatac ggaataacgc agatatgctt aaaacggcac
      781 tgcgttattt ttgtgaaaat gcacaaaatg ctattgacga aaacgattac atatgctatg
      841 atagtgaaaa gtgtatattg cggtttgtgt aaaaagtcaa caactgcaag acggaggaga
      901 acatggaaaa caagtataga ttcagggata gctcgctgtc attttacgag cgagtaaagg
      961 accttttatc aaggctgaca atcaaggaaa aggcaggact tatgtcaagt catatggcgg
     1021 ctgtgcctcg ccttgatata ggcgagtggt atgtcggtgc ggaggttgca aggggatatg
     1081 tttcgagaaa ccccgatgag ccgacgaccg tattccccca gcccatcggg ctttccggaa
     1141 cgtttgacac ggaacttatg gaaaaggcgg gacttgcggc aggcaaggag gcacgggtgc
     1201 tgaacaagcg ccacccgagc ggtcacctta tgctgtgggg acctaccgtt gacctgtgca
     1261 gaaatccgct ctggggcaga aatgaagagg gctacggaga agatccgttt ctgacaggtg
     1321 aaatgtcggc ggcatatact aagggacttg cggacaggca tggtgagtat tatcagtcga
     1381 taccgacatt gaagcatttc tgtgcaaaca atacggaaaa tgagcgagga acggcatctt
     1441 ccgatgttga gccgagaacg cttaatgaat attattatgc ggcgtttgaa cgtcctataa
     1501 cctgcggagg agcgtactcc ataatggcgg cttataatga gctgtcgggt gtgcctgctg
     1561 tcataaaccc cgatatacag aatgtgctga aagataagtg ggggcttggc tttgtcgtaa
     1621 cggacggcgg agatttttca cagaatgttg tattccacgg ttacagcact tctcatgcgg
     1681 aaacgatagc acttgcaata aagaacggca cagatgttct gaccgactgc gaagatgttg
     1741 ttgcggcatc ggtgctggag gcggtaaaga gcggacttgt gtcggaaaag gatatcgaca
     1801 aggcgcttta caacagtatg cttgcgcgtt tcagactggg cgagtttgat gaaaagcacc
     1861 cgttctcgga tattgacgaa agtgtgcttg actgcgatga gcacaaaaag ctcaatcgcc
     1921 gtgcggcgct tgaacagacg gtgttactta aaaatgacgg tatattgccg cttgatacaa
     1981 acaagagtgt tgctgtgata ggtcttaacg gtaactgtaa tctgatggac tggtacacgg
     2041 gttattcgtc atacaatacg actatacttg acggcataag cggaaaattt gccggtgcga
     2101 tgtatgacaa cggctgcgat catgtagtca taaagtcggt gatgacagga aagtaccttg
     2161 gtgttgccga tgatgataca gtaagcgcaa tttacgaaaa ggacgacccg agggcgctgt
     2221 ttgaaaaggc tgaatacggt catgacgaaa ccacttaccg ctcgctttac aataacaggt
     2281 acataaccga aagttcctgc aagtgcgaca gtgagagcac ttaccgctgg tattcgcagg
     2341 aaatcatgaa gccgcagaag cacggagata aggtgctgta ccgcacatat ttcggtaagg
     2401 cgctcggcgt tgatgaaagc ggtaagctga cgcttgtaaa acagttcgga ctcagtgata
     2461 acaagatatt tagtgaagaa attatttcag acggtataag acgtgcggct aagcttgcag
     2521 aaaaggcaga ttatgcgata gtctgcatag gtaacgaccc gatgatagta gcgcgcgaaa
     2581 tgtacgacag gaaaacgctt gcgctccccg cacacgattc ggcacttgcg agagcggtct
     2641 atgatgctaa caataactgc gtactggcgc ttgtttcaag ctatccgttc gctatatgcg
     2701 aggagcagga gtatatgcct gcgataatct acacgaccca tgcaggtccg gagctcggca
     2761 atgcgtttgc aaaggttata ggcggcgaat attcacctgc gggcaggctg gcgcagacgt
     2821 ggtataaaag tgaatatgag cttgctccga tagaaagcta tgatattata gaaaacgaaa
     2881 tgacatatct ttattataag ggaaagccgc tttatccgtt cggctatgga ctgagctatg
     2941 ccaaatttga atattcggat tttgacgtaa gggaaaacgg cgatgttata agcgtgtcgc
     3001 ttgatgttgc aaatgtttct gaaacggaca gtgacgaggt agtacagata tatttccgta
     3061 tggaagatcc gtcggtcaag cgtccgctga aacagctcac agcctttacg cgtgtgcata
     3121 tagagggtgg gcagacactg cgcgttatgt tcgatataag caagtcggag cttcgcttct
     3181 atgatgtcag ccgtgaacgc tttgcaatag agcagggacg atatacattc atggcaggtg
     3241 cttcaagcga ggatatcagg ctcacaaaaa ctattgatgc aagcggtgaa aaaataccgc
     3301 cgagagagct ttgcaagggt atatcggcaa agaactacga cggtaagtat aacacgaaaa
     3361 tgcagtatag taagaagctt gaaatgcact atatgctcgg tggaggactt atctacaaca
     3421 actgtgtgct cggtgacgca acgaacatcg agatagtatg cggcagtcgg gtgaacagcg
     3481 gaaaaataac cgtaaaatac ggcggtaaaa cgctgtgcga ggctgaggta aagcccacgg
     3541 tggatatcac aaggttcgat acggtgacag tcaagcttga cggcgaggtg cttgaaagta
     3601 acgacaggga taagcctgcg gtgtttgagc tttggatgcc cgaacagatc tacatactga
     3661 gtttcagggc ttattgatct ttatcaagcc cgcagtggca tttaagcacg gtgaaggtgt
     3721 ttttatcgac ctttatgcgg ccttctttta caagctcgga cagtctggct gacattgcac
     3781 tgcgttctac gccgagataa tctgccatag agcggcggtc gagcggcaca gtgaaggtgt
     3841 cgcttccggc tttcttggct tcgtggctta agtacgccat tatcttttcc ttggtggtgc
     3901 gctttgagat gatctcgacc ttgcggttga gcataagtgc gttatcggaa acgactgtca
     3961 gcagattgct tacaaactgc ggataaaacg gatatgacga cagggaggac agcttgacgt
     4021 aatcaaggag cataacggta cagtcgcttg cggcttctac ggtcacaggg agaagtttta
     4081 cggcggagca ggcaaacact tcggcaaagc tcccgccctg ccttatcatg gcggtaaggt
     4141 cgcggttgcc gtaataatcc tcgcggacga cctgtacgct tccggaaaga acgataccta
     4201 tactgtcgga ggggtctccc tcgctcataa ttgtttcgcc ggactttacg gtgactgtcc
     4261 tgcctgaaag cagctttgcg cataacgcag tgttttctgt cgacatatta cggaataact
     4321 tatttttttg cagaagtgcg agtgtttttt cgtccataga acctcctgtt gaagcttaag
     4381 cggcgataaa gcatattctg ccgataatga tataacttat gcgcgaaaaa gtcaaatatt
     4441 tttgttttat ccgcagctgt tccgagctgt cggtatactt gacttattgc gaataatgta
     4501 atataatatt taagaacgac taaacgaaaa aaggcgaagc acagctttgc ccggaaagga
     4561 ccattaaaat ggatgcaact ttaaaaaagc agcttgagat aactgccacc aaggtcagaa
     4621 tnnnnnnnnn ncatcccggc ggatcgcttt cggtagctga tgtactcaca tatctctact
     4681 ttgcaaagat gaatgtagat cctaaaaatc ctcagatgcc cgagcgtgac agacttgtac
     4741 tgtcaaaggg acacacagct cccgctctgt actctgtact tgctaaccgc ggatttttcc
     4801 ctgtagaaga tttaaagaca ctccgtcata taggttcaaa gcttcagggt caccctgtta
     4861 tgaacaaagt ccccggcgtt gatatgagca caggctcact cggacagggc atttcagccg
     4921 cttgcggtat ggctctttcc ggtaagctgt caaatgaatt ttacaaggta tacgctgtac
     4981 tcggcgacgg cgaaattgaa gaaggtcagg tatgggaggc tgctatgttt gcggctcatt
     5041 accagcttga caatcttgta gctgtagttg acaacaacgg acttcagata gacggcagaa
     5101 taagcgatgt tatgtcaccc tatcctattg acgagaagtt cagggctttc ggctggcacg
     5161 ttataacaat agacgcacat gattttgacg ctatcgaaaa ggctttcgca gaggcagaaa
     5221 aaatctcggg acagcctacg gtgatcatac agaagagcgt aaagggcaag ggcgtttcct
     5281 ttatgg
//
LOCUS       DAWAOG010000073         5283 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2040_length_5283_cov_2.726664, whole genome shotgun sequence.
ACCESSION   DAWAOG010000073 DAWAOG010000000
VERSION     DAWAOG010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2040_length_5283_cov_2.726664"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4070..4079
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1322 a         1372 c         1129 g         1450 t
ORIGIN      
        1 gcctttatat tggcttctat ttcttcaatg gacagctctc tgcactttgc atagctgccg
       61 taatagccca tatcggcttt ttcggcctcc tgtgcctttc ttatttcgtc aagctcctcc
      121 gctgaacaga agcacggata tgcaagaccg cgctttacaa gatccttagc aaaggtctgg
      181 tatatctctt ttctcatact ctgtctgtac ggaccgtagc tgcccttttc aggctcgccg
      241 tcagccgccg caccctcgtc aaagcatacg ccgtactgtg caagagatgt gattatctcg
      301 tctgctccgt gttcgatctc acgctttttg tcggtatcct caagacgcag gtagaataca
      361 ccgtccgact gccttgccat acacattgaa ataagtgcgg catacagacc gcctatgtgc
      421 ataaagcctg tagggctggg agcaaatctt gtcacagccg ccttgtcggg aagtgttctt
      481 gcgggatatt ttgcaaacat atcctcgggt gttgtcttaa tgtttccgaa cagaagctcg
      541 gctaaataat tgttgtccat tttttcgccc ttcgtttatt ttatttttta atgtgcttct
      601 tagcgacctt gagtatcagc acgatagccg agctgagtac cagatttatc gcaagctcta
      661 caacaaagtt aagtcctaca aatgcggtca ccatatatac gccgacattt tcaaatcctg
      721 cgcctgctgc ccactgtgca atagtgtcat aaaagaacac aaggcagcct aaaaggaaaa
      781 tacccgtatt tactatcggg gacacaatgc ccgcgcctat tactccgagt atatcgttct
      841 tacggctgag cagactgtag ataagtcctg cggcggcacc tgcaagagtg ccctttaaaa
      901 gtactgtaat tatcgttccg gggacgttta tcgcaagaaa agctcctgcg tctgtaaaca
      961 gtacgaccac gccgaataca aagccgagcc atgcacccgc cttccagccg tagagagccg
     1021 caccgaccac tatcggaacg agtacgagcg taatgttgaa tacgccgaaa cggatcgcta
     1081 tagccatagc ctgtaatacg actactatag ctgtcagcag tcccattgtt gtgagcatca
     1141 tagttttgtt ttttgtagat gcaagtgtgt tgtttgcgtt tgacataaaa atctcctttg
     1201 ctgaagtccc attgtcagtg agtacctcgc agggttgtat attttacata tcatctgcaa
     1261 atcagaattt gatcacagcc ggtaaccgca gaaataacat ctgtaggttc cgtccacagt
     1321 gccgtttact tcttctgatt tttcttgtag ataatatgca gaccctctaa tacaaggtta
     1381 ggatcgaccg tcagctcgcg cctgcaaagc ggagctataa ggcttgcata acctcctgtt
     1441 gcgactattg tcgccttttc cccgagctct tcctcgtacc gctctatcat accgtctatc
     1501 atgcaggcaa ttccgccgat aactccggca cgcatactgt cggggctgtt tgtacctatc
     1561 atctttttga cggattccgc accgataagc ggcaaagatg ccgtcttgcc ggaaagcgcc
     1621 tcgaatgata atctcgggcc gggcgctatc gcaccgccta gaattgcgcc gctcttgtca
     1681 agcgcgaata tcttaaggca cgttcctaca tctatgaata tgcacggcaa gggatacttg
     1741 ttcttagccg ctacaccgcc gcatacaagg tcactgccga gtacggacgg atcgtctatc
     1801 ttaatgttaa gtcccgtttt tataccgggg gacacaacta taggctttac attacagagc
     1861 ttttctatac ctctttcaag aacaggcgta acaggtgata cgacagagga aatgatacag
     1921 ccggttatag ccgcattttc aaagccgtaa agggacagta tatccctgag ctttatcgca
     1981 tactggtctt ccattcttga aacctgtgtg ttgagccttg atataaagca cagctcacct
     2041 ttatcgtcaa agccgccgaa aactatattt gtattgccta catctactgc aagtatcatt
     2101 ctttgccctc cggtttttcg gatatatcgt tttctattat agcatcagtt tcgtcaatgt
     2161 caatatcttc agccttattt aacgctttcg taagctttac aggcttcttt atcttaagat
     2221 atatcagtat catacccaca aagatcgccg taccgactac tgtcagtata attcctgtgc
     2281 cgagtcctgc cgcgaagaat ttaaactcga caacgtgctc gccctcggtc agctctactg
     2341 tcataagtgc gtcattaagc gcagtgccgt aatccgtctt tacgccgtca acatacaccg
     2401 tccagccctt ttcggcgggg atagtcgtga acagcatatc gccgctctta gcgtttaccg
     2461 taaagcgtat atcggtctga cttacctttt cgaccgttgt atcggcgttc ttttcccaca
     2521 tctgtgctat tgccgctgtt tccgcgtcct cgttataaac tgcgaactgc ggctctctga
     2581 aataaagatc actctttttg agaacaagct ttacagtgac ggtttcgtcc ttttcaaacc
     2641 tgccgagatc cttaatatta tggttgtcgc tctcaaagca tataccctta tatgctccgt
     2701 ttacataaag cgcggcttcg cgctgataat cggtcggcag gaacatatac atctcgccgc
     2761 ttttcaaagc ggtgacggta tagcttacac ttgcttctcc cttagcttcc gtaaatccga
     2821 tatggctgtt ttcaaacgag cccttcttgc agttttcggc ataaggctcg ccgtctgcgg
     2881 agaacaccgt gaagaactcc ctgttgccgc ccgtcatggc gttaagcagt ttattctgat
     2941 tgtcgaatac gtcatacttt gacagctcaa gcgatgatac cttgctgttt acaagatagc
     3001 ctaccggcag agcgtccttg ttttcggtaa cggttatatc gtccgcagac cttatgtttg
     3061 cggtcgactc gccctgacag tcgaggatat actttacacc gaagatatcg cttgtcagcg
     3121 gagtgttacc cgaaaatctc gaatagtgtc cgttagaggt gaagccgaaa tatcccagca
     3181 tatctatagc ccttgcatta agcgtactgc ttgaatgcga cagtccgtac atatttaccg
     3241 ccatcgggtc gtttaccgaa cggaagaagt tcttttctat cctgtagaag ccgtcgtcct
     3301 gctcctttat ttcctcaacc ttttcacgca ggggaagtat aacgctcaag tagctgtccc
     3361 ttgtcgagaa tgttatatcc tggtgcatct ttgtaagcgt atttgtagtg ttaaagcaaa
     3421 gctctgcgca tatgaccgcc gttacaagga taacgcccgt ctttgatacg tttttcccgc
     3481 cgccgtaatg ccttaccgca aatacggtaa ttcccgcaac cgccataaat gctatcgccg
     3541 ggagcgcaac ggttatgccg tcaaatacct ctctgccctc actgccgagc gtggttatga
     3601 aagtatcctg tgcttctgtg tatattatga gagcaatata actcagtgta atcgctccta
     3661 cagtccttgc cttaacctct ttcagctgtt caaagcaatg cgccgcaagt gccactatca
     3721 caaacgacag cataaacgag tagcggtaag gaagccagtt cggcatctgt ccgccgtgcc
     3781 atatcatatc gacaggacgg atatacatac ttaccgtcag cagtgcgata agcgtaccgg
     3841 ccgcaagctt cttcttgaag tgatatgcct tacagcagaa aaatcccgcc gcgagcacaa
     3901 gcgccagcga cccacagaat ataaacggca acccttccat tcttaccgtg tcgtaagtat
     3961 tgggaaacag ctttcttgct atatctacaa ggttgaaatt attagcaagc gaatagtcag
     4021 gctcggtaaa gctgaacttg ccgttctgta atgagttgtc aacgggcagn nnnnnnnnna
     4081 atgcgctcat catagccgat actattccgc acaccccgaa gaacgcaccc tttgtgacaa
     4141 atttctttac tatgcccgct ccggtgctgc ctgtataggt ttcaagcgag aagtaacggc
     4201 agataaagta cagcaccgag aatatcgccg ccataaagcc tatgtagaag tttgacacaa
     4261 atgcatacac aagcgagccg ataagcagtc ggaagcggtt ttcctttatc agcgaatcta
     4321 tgccccagca gataagcggc agggcgataa cgcagtcaag ccacataggg ttcatggttt
     4381 gtaccaccat atacgcacac atggcataca ttggcgcaaa cacagccgca gtgatctttg
     4441 acagcttctg cgacttatgc gcaaacagtg caaaagcgac accgcacgca ccgaactttg
     4501 ccagtatcat tgtgagcaga ccctcggtta tcatactgcg cgggaatatc cacaccagta
     4561 tgttgaacgg gcttgcaagg tagtagccga tgatacccat aaactcgccc gacaggtttc
     4621 tgctccacga gtacatcagg ctttcaccgt tgcctattac gtcatgcaca tagtcatagt
     4681 agaaaacgta ctgcgcgttg aggtcaagcg acagcaccga cttttcaccg aaagggtata
     4741 taccgaatgt gacatatgct atacccatta gtatcacagg gataagaaat gccgcgaaca
     4801 gataaccgtt cttatagcac aagtgcctga atgacagcat tttttctctg aaagaagcca
     4861 ttatttttcg tcctcacatt tttgtctgtg ccccgactct tcacttacta tatatctcgg
     4921 gcgctgtttt atctcttcgt atatcttgct cagatagtat ccgataattc cgatgccgag
     4981 cataagtata ctgcctatta tcagaagcag gagtataacg gtcgaaaaac cgtccgccgc
     5041 tgtccccgta cagaagttga ctatcgtctg tactgcaagt atcacagcga ataagaagaa
     5101 tagtataccc gaccaggtga tgatgtgcat aggcacgttc gtaaagctgg ttatcgagct
     5161 taaagcatac ttgaacagct ttctgaacga ccacttggtt ttgccggcgt ttctcggtgc
     5221 aacgtcaaac tccacacgct ctgtcctgaa gcctacccag cttgacagcg cacggaagaa
     5281 tgt
//
LOCUS       DAWAOG010000074         5265 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2047_length_5265_cov_2.745298, whole genome shotgun sequence.
ACCESSION   DAWAOG010000074 DAWAOG010000000
VERSION     DAWAOG010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2047_length_5265_cov_2.745298"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    652..661
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    915..924
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4579..4588
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1296 a         1345 c          963 g         1631 t
ORIGIN      
        1 ctcggcttat gaacgctctg tttgttttgc gtcctgcaaa actctgggcg ttcaatatcg
       61 ggcatccttg cccgtccctt gccaaaggga gctggcttac ggcaaggttg acatttatca
      121 aatgcttcca ttcttcgcga cactcgcaac actcaaataa tacatacctt atctgccgtc
      181 aagactgagg gatcgcctgc atcgaacctt tccgcttctg ccatcacctc cgctaacaat
      241 ccctcagtca cagtatcaaa aagctcggtt ctgctctgca catctttaac ctgatactca
      301 cagcaatacc taaaatgttg taccgcactg tgacagctcc ctttacacaa gggagccaag
      361 tgtgatactt ctttttctca atttgcactt aaccccaaac ctctataaac ctcaatatca
      421 ttcaaaaaag aaacaacacg ctcagccaag cgctaattcc cggctctctt gccaaaggtg
      481 agcttctgtt gcttaagtct tgctcagcca actattgagg gatcgccgtc ccgattatct
      541 ccacttccac aaacaccgct aaccccgcac tatccccacc aaaaaaccgg gcaacgattt
      601 tcccatcgct acccggtctt attattaact tttattctgt agcaattact tnnnnnnnnn
      661 ntcttagccc agttcactct taaagcgtcc ttagccttac cgccgatctt aacacactta
      721 acaatcgagg gatttgtcct tgcggttact gtagcgctat aattaccgta aagcgctgtc
      781 ttaccgctca tcttatagac tctcactctg aacttgtata cagtcgaagc cttaagtccc
      841 gcttttctga aagtggttgt gttattgttt gttatcttgg caattcttac ccacttatta
      901 ccctgataca tctcnnnnnn nnnncaacta tatatccgtc agcgctcgca ttcttagtcc
      961 agtttatcct cagcgcatca gcggctcttc cgccgagctt tgcgcccttt ataacagacg
     1021 gatttgtcct tgcggtaact gtagcactgt acgcaccgta atccgcatca cgtccgtaca
     1081 tcttgtaggc tttgaccctg aacttataaa cggttgaagc cttaagtccc gcttttctga
     1141 atgttgttgt accgttgcct attattttgc caacgcgcgt ccatttgccg ttctgataca
     1201 tttcaacgat atagccggtt gcatttgcat ttttcatcca gtcgatacat agcgcgtctg
     1261 ccgctctgcc ggagagtttt gttcccatta cttcagcaag tataggtctg ataagcagtc
     1321 catatttaaa accgtatttt ttggcgtact tctcaccggc tgtgccattg tagcaataga
     1381 ttttgaagtt gtcgtatttg acactcccaa aatttttacc gtcataatga tagccaaaag
     1441 cttgttcggc tacatctata acactaccgg gtatcgttac acttgtaaga tttgcgcaac
     1501 cgaagaaaga atttccgcct atagtcgaca taccatccgg tattgttata cgtttaagac
     1561 ttgtgcaatc gctgaaagcg cgctcatata tgcttgtcac accgtcaggt attgtatagc
     1621 tttcatcttt ctttccagcg ggataacata tcagagcagt tttattctta gagaataaaa
     1681 tgccatttaa agaaacgaag tttggatttc cggcggctac tttaatttcc gtaagactta
     1741 tgcaactgca gaaagccaaa ctgcctatgt ctgttacggt tttcggtatt gttatacttg
     1801 taagacttgt gcaatcactg aaagcgcgcc aatatatgtt tgtcacaccg tcaggtatcg
     1861 tatagctttt atctttcttt ccggcgggat aacatatcag agtggtttta tccttagaga
     1921 ataaaacgcc gtttaaagaa acgaagtttg aatttgcggt ggcaacttta atttccgtaa
     1981 ggcttatgca tccgcggaaa gcctgatcgc ctatgtttgt tacgtctttc ggtattgtta
     2041 tacttgtaag gcttgcacaa ttgtaaaaag cactgtcgcc tatatgttcc acactatccg
     2101 gtatcgttat acttgtaaga tttgcactat ttttgaaagc ttcgcctgct ataatcgcca
     2161 caccgtcagg tatcttatag cttttatctt tttttcctgc gggatattgt attataacgg
     2221 ttttatcctt attgtataaa acgccgttta cagaaacata atttaaattt ttcgccgaaa
     2281 ctttaaatcc tgtaagactt gaacaaccgc tgaaagccca cgcccctata tcatttaatc
     2341 tttccggtat tgttacgctt gtaagacttt tgcaattgct gaaagcccac cagcctatat
     2401 tcgtcacgtt gtccggtatt tttatacttt taagacttgt acaattcata aaggcaaaat
     2461 agcctatact tttcacgctg tccggcagtg ttatacttga aagactcgtg caaccgctga
     2521 aagtgctttc ctgtatgctg gtcacaccgt ttggtatatt tatacttgtc aggctggagc
     2581 aaccgtagaa agcactcgat tctaaaactg tcacactatt tggcactgct atgcttgtca
     2641 agcttgtgca ttcggcaaaa gcaccgccga atatagcggt tacgctgtcc ggtattgaaa
     2701 cgcttgtcag gcttctgcaa ccgcagaaag tggtatacat tatttcggta ataccgtccg
     2761 gtatcgttac gcttgtaaga cttgcgcaat cttcgaaagc tccatcgccg atgctcttca
     2821 cactgtccgg aattgttata cttgcaagac ttgcacagcc gctgaaagcc tcattgccta
     2881 tacttgtcaa tccgttcggt atcgttatac ttgtaagatt tgaacaatct ctgaaagcat
     2941 cattgcatat attcttaaca ctattcggta tcgttacatt tacaagattt gtgtgacccc
     3001 caaaagcgtt agagccaatg ctcgtgacgc tctttccgtc cattttttcc ggtatatcaa
     3061 catttttttc cgtattgcca aagtaatacg caatctccac gctgccgtca gcaagctcgt
     3121 cgtactcata atccctgtac cactccgctc ccgctccaac ggcgcacacc accgccataa
     3181 ccgcgaaagc cgcaatagct aaaaaccact ttttcagttt cataaaacac cctccttata
     3241 ctttagtttc tgtgtgagca tggcttaatt taataatata ccaatatctt catttgtcaa
     3301 cccctttttg tgaaaccaac gacaattttg caaaaatagc ccaaataact ctcatataat
     3361 ctctcaaaaa ttaaaaacgg ctttgttgct cgcaccgttt tttaatctaa cgcagtcccg
     3421 attatcacta cttgtacata caccgccaat cccgcactat cccaatgatt ctgtttcaca
     3481 aatcgcactt gactaaacct ttatatctcg gtgagggtct gtgtgtgctt gtatggtttt
     3541 gcgtccgttg caatcgcggt cgtttgtggt cggtgtgtcc ctgccacgtc agccatttgt
     3601 gccgttgcac ctgttccctt tgattctttc cctcgttcat agcagcaaca ttattgcgca
     3661 gaatagcaaa cccgagaaat actacccgac caaaagaact atcgatagcc tgctatcaac
     3721 ctgaatttct aatacgatat aacagaattg cactaaagac agactttctc cacatatcaa
     3781 aaaagtgagc cgtgcgcact atcttttgcg cttcggctca ctttttcttt ttgggttatg
     3841 tcacaacccc tttttatatt catcttattt taagctcaat atactcaatc ctgctgagtc
     3901 gcgctcagct tatccgcata ttcccttata accttcctgt ccgagctgtt cactatcttg
     3961 tatgttgtcc tcgaatagaa atggctgttg gtatcccata tgaccggaac tataccaaga
     4021 tctgtgagct ttccgagtac gatcgacaga cactcggaag agtcggtgta ctccgcgtcc
     4081 tttgcaaagt tctcctgcgg atatgtagag gtcgtttcgc ccatgaatac ggggatatcg
     4141 ttgtcaagaa aagccttttt aagctcggca cactggctgt cgatgtactt cagccattcc
     4201 tctccgccga tcttgttcgc ccagtacata gagttgtcaa cataatgcac cgacaccata
     4261 aggcggtcgt ttaccagatc ctccggcatc tcaaaccttg atgaagttgt cttgtcgata
     4321 ttcgtccagt accccgaaac gataagcacg cgctgtgcat tatatccgcc agtgcctctc
     4381 accgcgtcta caaaagcctg attgagcgta ttcgtcatct tgtacgcgtt ggtatcggac
     4441 tgcgccttaa ggttgccgtt tgagtcgaac tgatcgccgc ccagatattc gttatatccc
     4501 tcgaaaacca gtgtataagg gtagtccgca aagtattcgg ctatctgcgt ccacagatgc
     4561 gtgaatatct ccttgcacnn nnnnnnnnat gataaactca tcgaagtggt ggatattgat
     4621 aacagaatac acgcctgcct tttcgcagta gtcaacgatc tccttaactc tcttaagata
     4681 ttccttgttt atcgtatatg taccgtcatt ctccatcata ttgccccaga acacaggaat
     4741 acgcacagtg ttgaagccct ctgccttcat tccgtcgatg atctcctgcg ttgtgacatc
     4801 tgctccccag caggtttcat agtcctgcgg ctcatttccg ccgacaacgg ggatccattc
     4861 gtaagacatc ttctcgcaat cggtagcctc atacgcttcc atagtgttgc caagacttat
     4921 gccgagtccc attttttccg ccatctcagt tgctgtgatt atctcatagc gagagtcgtc
     4981 cgtatctggt acaggtgctg taccgttgtt cgaaccatcg gatacattgc tttgtgcacc
     5041 ctgactgtct gtgcctgatg tgacattgcc cgcacagcct gtcagcataa gcgccattac
     5101 cgtaagtatt gccgttaatc tcttcttcat aagaatacct ctttgtacaa aaagagccgt
     5161 tacatagatc tatgcaacag ctcttatgtg tctatgactt atttcgcgta atcaatagct
     5221 ctgctttctc ttatcagatg tacctttata tgaccgggat attcc
//
LOCUS       DAWAOG010000075         5264 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2048_length_5264_cov_2.312920, whole genome shotgun sequence.
ACCESSION   DAWAOG010000075 DAWAOG010000000
VERSION     DAWAOG010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2048_length_5264_cov_2.312920"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1436..1445
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2091..2100
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2745..2754
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3158..3167
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1401 a         1084 c          928 g         1811 t
ORIGIN      
        1 atagctttat ccattacgtc atgcagttca acttcactta acagatactc ggtttgctct
       61 cttgctactg ctgtagaaat tcctttgaga gctgatatat acatcataaa ccgtcttgcg
      121 gtaaagttgt cataaagcgc ctgctgttgc ggcatatatc cgaccttacg gcgaaattta
      181 ctgccaagct tagttatgtc tgtgccgttc cagcaaagct gcgagcctaa gtctggtgca
      241 aggttatctg aaataatgtt cataagcgtt gattttcctg cgccgttggg gcctaaaagt
      301 ccgtaaaccc cgtttttaaa ggtcatctca aaatcaaaaa gagcctgttt tttaccgtaa
      361 tgtttattaa gatttttaat ttgaagcatt ttttacacct ctacttttta gcctgaacat
      421 tttccgcatt ttttaccgta aaggaaaacc tgtccagact atcgctttgc catataagct
      481 ttatatcgct gaccgttaat tttgctgtac caaacggttt atttctgaaa tcctttgtga
      541 gccactcgct gttatctgtg ttgcctgcat aaaaatatac acaatcgcca tatagcaata
      601 cacttgtttt agaagcattg gaggtaagat aactactctc tatatcaccg gaaaacctcg
      661 gtgaaaaacc aagtgcattt tttaagcttt cgggataagg aaaaaacaat atgctgtttt
      721 cttctgatat gtacagatct tcatcaagaa ctacatacaa tacgccttca agagtaacct
      781 ctccgcttaa tgtgatttct gtgtatactg tttgcgatat tcccggctcg gtagaatttg
      841 tttcccgatt gtattcgagt gtttctttaa cggacgaaac tgcagaggtg acggtaagcc
      901 cactttctgt aacatcaccc gatttcagca attttatcat ttttttgctg tcctgcggca
      961 cttcaccttt gtaattcata ttatcatcga atttatcagg agaagtaaaa ctgtcacagt
     1021 aaaaagtatc attaatttta cgcacagcgg caaatggtgc gttccatagc tttttattct
     1081 cgtcataggc agcaccttcc ttgactattt gcagaccgtt ttcgtcaaga ataaaatatc
     1141 cgtcgccatg atatatttct ccgacaagac ttgtaacact tttctctttg ttactacttt
     1201 gagagctttc tgttacatct gaacaacttg agattaacat aaccaccgca attgtcgcca
     1261 ctattaaagc tattctactt aatccttttg ccatatctta ctccactaga atatccgatt
     1321 ttgctacctt ttcttttgtt actgtttgtt cttcggcgtt atagacgctg ataatatagc
     1381 tgtcctcgta ctcggacaat acctgtgcat aaagcccttc gccatagctt atatcnnnnn
     1441 nnnnnatcat gccttgcggg agcttcatcc tttaaattat aataatacca ctgtgtattg
     1501 tcgcattgga acagataatc gcccaaaatg acagtttcgg acaatatcct gtcgttatga
     1561 ataacaatcg gttcagaggt cttcggagtt aacacttcaa tattgctgtc attgttatat
     1621 gcatagtaat catcgctaag tctgacagca ggcggctcgg acggcatagt gttttcacta
     1681 tactttcctg tcttaatgtc gtacaccgag ctgttgaaat aaaatttgtt gtcatagaca
     1741 cctaaaatct ttgttttatt gttttccgtt gcaccaaaat cggtaaattt tccggtttct
     1801 atgtcaagca ctccgaaatg attggtatta tcgcctgtaa tcgctccttt ttcgtcataa
     1861 gcagactgat aagggaaaaa cagcagggtg ttgtttttaa gtacgcttgt gcttatccag
     1921 tcgcaggaaa agccttctat tttgcatact tccttttcgg aagtaccaag cgggtctgat
     1981 gagtgaagta ccgcggattc tactgctttt ccgtctttgg tttcgtatct atcctcgaac
     2041 cagtaaagct tttcgttata caaaaaaggc aaggtttgtt tgccgtaagc nnnnnnnnnn
     2101 aggcacggaa gtccgtcctc tgtaacagca tttgagtggt cacagtttga ctgaccgcat
     2161 agcgcaattg tactcatatc agaatactgt aaaaagctta gtactgcgtt ttcggtaact
     2221 atcaactcgc cgtcattata tattgttccg tatactcgca tatatctcgc ctgatcatct
     2281 gtttttgtat ccgagcaggc ggaaaaagtg gacgcaaatg tagcaatcag tattgcagtg
     2341 cctataagct ttaatttcct gttcataata ttgccctttc ataagttgta tttatgtttt
     2401 tattttattt tctgtgtcaa aaaatataag cagtaattat atttcacacg caggagcatc
     2461 ttttgtttta tttccttttt tgtcttatag atacatttat aacacgtcat gttgtgccac
     2521 cgtacgtgca gtcgctaaac aaaagttaat atgtgtagct gactgcccag aatggtttgt
     2581 atgtaccaga agtgcaattt atagatatat ctacaaactg tattttatat cctgctgctt
     2641 tttcagcagc agtcaatgct actgtttttg tcattttatc aagtctggaa tattcgccgt
     2701 taccgttact cttattcttt atgacagttt ctccattttt tgtannnnnn nnnnctgaaa
     2761 cgcttttttc gccgtttgga acctccgtcc caccgtcgaa tacatatttt gcagttgctg
     2821 tttgtttact ttgattaata gtgtatgaca tgactacgca tgatgttggt cccataggaa
     2881 ccaacttgta gtttgatgct gctttgtaag tgacaggtga taatgcgttt cctggtaaca
     2941 ccatagcaaa tgccgatgaa attacagctg ttgttgctat agttgctttt attatgtttt
     3001 tgattatcat catattatac ctctttcttt ttcccaagct cacatatatt atccaccgat
     3061 acaacagtac cgattaatcc gctcataccg cctctttctg tggatatata agtaatctct
     3121 gaaaatgtta ttgtggcctc tttataatac acatcctnnn nnnnnnngaa cttaaaaagt
     3181 cgcaatcctt taaataaccg atatttataa caccatcgct tgttgtaatc gcaaactgcg
     3241 gattgcccca gctgtaatta tagaacatag cacagctact ccctttatat tgagcgtcaa
     3301 catcaaaaag gaacggcaca gcctcatggt attccttaga ataaggcata aacagtattc
     3361 tgctttcgcc atcatgctcc gaattctcta tattgtagcg ttcggcaagc agtatacccg
     3421 taaaggttac ttcacccgtc agagttattt ttgcgtccat tacatcatag cctatgccaa
     3481 tatctttttt tagcgtttcg ctatacatta cgcgaggtgc tacgctgatg tagcacgagt
     3541 ctactatcag acccttactg ccgatgctgt cgccttcatg tactattatt ttatccttgc
     3601 ttaccacggg tcctttttga gagcctatac atttcatttc gttcaaatcg taatcatcag
     3661 gatttcttat gctgttgttg tcaaacggtt catattcata accgcccatt gaaccacgga
     3721 taatatatga acctgccgca ttgggatcgg cttcaaggtc agcaagcttt acaaattcgc
     3781 cgttgccgag gtacatttca ttttcggggt cttcggtata gaattccggc aattcctcca
     3841 caggttcttc gatagatgta tcgcctttat taatatcctc tatgagtttt atgtattgta
     3901 atgcgtcctc acaatattct accgatttaa tactattgtc tgtgtttaat ataattctat
     3961 agccttgttt ggttttctca tctgcataaa catatacatt atctccgctg tctgtggtaa
     4021 aggttttgaa tttgttgatt tctatttgct gaatcggttt tttgtcgtta tctttaattg
     4081 aaataaagca tataaacggc tcatcggaac ccgatttata tccgtaaagc tccacggtgt
     4141 tattattgtt atcggtaagc gtttttacaa gctcactacc ttcgggtgct gttatactcc
     4201 cggtttttga agtatcggta acagtatcga tactgctttg taaggacgtt gtattatcat
     4261 ctgccgctat actactgttt tgttcgggct gagaacagcc tgttaaagct actgtgaccg
     4321 ataaagccat aaccaacaat attaatctta atctgtcatt catattaagc tcctttatgc
     4381 atataatcga gtcgaggata tcttttggca aaattcttgt atgtgaaaac ctgtttgttt
     4441 atttggcaat gtcctgaata atttgacgat ccagcttcta ataagctgga attagatccg
     4501 cacccgttag catcataaac tgtaattgtt gtatcagttt ttcccgctaa aactatagag
     4561 tggttattat tgtttgatgt tcttactctc aaatgcgtgc ccaccggtaa atcggaaatc
     4621 ataattttca agtttcttgc ggttaggaat aaataatcat tgttgtcgct tccccaacca
     4681 aaatcattac attttgattc agataaattt ttagtaaata cacctttata cacaaatgct
     4741 gcaaacccat agcattgata acaaccgtca aaacatccac atgttgcagt tgatgcctca
     4801 ggactgataa cactatgtgc tgaacaagat gatttaccat ttacaaaata cttgcctgca
     4861 gtaagattaa catttttaga accatcaaca taatagcagt aatcaatgtt gccattatta
     4921 tatttgattt taggggtgta gcttgtaaaa tgtgttgcgg cactggacga tgatgcaacc
     4981 gctatagaga tcgccgcaat tattgacaat gatgagatgg ttataaatat tctttttgct
     5041 ttatgtatga acttaagctt catttgataa tttcctttca ttttaactcg gaataaagcc
     5101 cgatgtaagc atagcttttc atcggctgtg acgaagaaat cttgccgcag gagcgttagc
     5161 tcctcagctt ttctgtaagg cagtaattca cctacgatag tctgaatgga acatgccgcc
     5221 tataaaggcg gggaacattg cggcattagg ttccaacttg atac
//
LOCUS       DAWAOG010000076         5192 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2082_length_5192_cov_1.994744, whole genome shotgun sequence.
ACCESSION   DAWAOG010000076 DAWAOG010000000
VERSION     DAWAOG010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2082_length_5192_cov_1.994744"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4216..4225
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1199 a         1265 c         1111 g         1607 t
ORIGIN      
        1 aatgtaattt gataagctga agaattgtgt cacagctcct ttttatgcat aaatacagcc
       61 ccgaccttgc ggtcggggct gttgtgacgg aacaagaaga acttgtagcg tttagctctt
      121 tgtaaatttt atcgcaagcg atacctcaaa gacatcgtcg cctgtaggaa ttgtgacatt
      181 tccgtttgtg actgtcagcg tggcgctttc gtatgacttt gtatatccgt ccatgaatat
      241 atctgggagc gttacggttt cacctctgtt cgtgtaaagc atataacgtg tttccggatt
      301 gccgtcaaca tacacttcta cgccgagcat aaccatagtg tcatcgttct tggcaaatct
      361 tccacagaag taaacatctc tgtcgggcat tgtgtatctg ccttccttat tcacaagaga
      421 agcgtccgtg catatccact tttcgttgaa cagatagcct tccttgcgcg gcaggtcgat
      481 ataagcgaac atttccttgc ctactgggac atcggtgata gtcatatacg gcgttgcttc
      541 gccatctatc atatagtaga cgttgtaggt tgccgttgtg taaacgcact caacgcttac
      601 atcgttatga ggcattgtaa acttgtattc gccgttttcc tcataaatag caacatcatc
      661 ggactgccag tagtaagtat agccgctctt cgccggaggt tcgggaagag ttaccgtact
      721 catatactgt gctttcatcg ggctgtcgga gcttgcgctt ccgtcaaccg tcatattgac
      781 attatagaac ttaggcgatg aggtcgcctt gaatgtaaca tcgtgagcag gcattacaaa
      841 cttgccgtct gttacagtga taccgtctgc agaccattcg gtcacatcgt aataatcctt
      901 tgtcggcttt gcgataaggg ttatcgtatc gcctatcttg ccgacctcct ttgtgccgta
      961 taccgtaccg tcaaattcgt aagatatata gcactttgtg gtcgtaccta ttgcggtaag
     1021 cgttgacgca ggcatcgtgt cgggctgtcc ggataatgcg ccctcagtgc cgtaccacga
     1081 ccagccgccg aagatatatc cgtctgcttc ttttggtgcg tcaaatacgg gaagtgcatc
     1141 gccatagtga taggtcactc tttttatcgt cttactatcg aggacaaagg ttatcgtgta
     1201 ttccttgtat gcgccgtcta tccataccgc tgtaagcgtc atatcctttg tcagcgttat
     1261 cgtatcatct acagcataga cagtgccgtc ctcggctttc caaccgctta agatataatc
     1321 tctcaggtcg tctgtttttg acgcttcagc catatccgac agcttgatct gagtaccgta
     1381 atctgctgtg cgttcatcgc ttcctgtcag cttaccgccg tttgcgtcaa gctttaatgt
     1441 gtgcttgtga acatttttct gccagttgcc aaagactatg tcaagtgtca gaccgtcctc
     1501 ggacgctttg cttgatgagc ccttgaacac gagagtatat ccctcgccct caacattggc
     1561 gggcgtataa ttgttgtact tttcaacgaa tgcgagataa tcatcatatc cgccgctgaa
     1621 tgtatcggtc ttttctccgt tcagaagtac accgtgcggt gtggatacat ttaccgtata
     1681 ggtcttgggc gtggcttgat agatcgctgt cagcgttgtc gaataatcga gctttaccgt
     1741 ctgacctaag gcatatatct cgcctgtagt gctgtccttc cagcctgtag gctgataatc
     1801 gttatacttg tcggcatcct tagttgccca acgtgaaatt tctacctcgt atttatcctg
     1861 gctgtagctc ttgaactctt tgctttcttc aaatatcagt ctgccgtcgc ctgcgtccag
     1921 cttaattgta acattacgtc tgaatctcgg gcggagtata atactcttgt aaatgagcgt
     1981 cttgtcgggg ataaactcgt tgaggtaagc atcaccgtat tcttcactca ggtatacatc
     2041 gctccagtcg atgaactgaa tcgttgtgcc atcctgccgg ttgagcttgc tccacgcctc
     2101 gtcaaatacc gactgaggta cctttgagcc gccctctatt ttcagcgtct tatatgtctt
     2161 gaattcacgg gcaacatcat cgtaatactg aatctcaaca tcgaatattc cggcttcata
     2221 aaccgcatcg tatacggtat cctcatatac ataaggcaat tgctcattga atacggtatc
     2281 cttatcgtat aaagttttac cgccgtccgg ggtgtagccc atgaatgtct taccgtaagg
     2341 ccagcttgaa ataaagaatt ccggtcttgt gccgtaaggt gtctttacgg ttgtcgactt
     2401 cttatcgccg gcataattat agtaattgta cgttacgttc atcagcgctc tgtcgtaatt
     2461 tgcactgaag tgaagtgcaa ccttctgata gtcgttgtga tgattgaaat agaagttttc
     2521 ggaagccgct ctctgggtgc tgcctgtaac cttgtaaagc ggttggtctg tcggctgacc
     2581 gctggtatag taatccttgc ggcatccgga gtctatcgct ctcagcttat ttccggcata
     2641 cttatacatc ttttcaaaac cgaacggtac tccgtatgtt gcgtgccagg agcttagctt
     2701 catatactcg tcacttgcgt tatcgggtac ttcaagttca tcgccggcat tcaccgccca
     2761 cagttcgact acccattctt tatttacggt gtcatagtat tccatctcca taagagtctg
     2821 cgctcttgtt gcggtgtata ctatttcata gttgtcgtgg tcaacggcta tgaagtatgg
     2881 gtctttgtcc ttccacttgc agtcaatgta catccagctg tcaggagcgt tatttgtgtt
     2941 gtaagagcct gcaagactgc ccgcaggtac ttttcgtgtt tcgacaagcg ttttaactcc
     3001 gtcaacctct ctgtagaagt ttacggtgaa gatcttgcct acgtcctctt ccttaactct
     3061 cgactcatcg taccagatga cctttaccga ctttgtaagg ctcatttcgg ggtcggcggt
     3121 ctttgtgaac tgaagtgacg tgcctgtata gtgtatctga ccgacacctt cggctgtact
     3181 gttcttgtag aagccggaca tactgaatgg ctgctcgtaa cggataaatt tgcctctcct
     3241 gtcatagagc attctgaagc cttcacccga aacgttcatc gacagcttac tccacggtac
     3301 agatgcattg taatttatcg cacctgtcgt gatatcaagt gtctgtgcgg acactgcggg
     3361 aagattattc acattgatct ctccccagta agtgccgttt gagctgaata atatcggagt
     3421 tgtcacatcg ttaaggcata taacaaggtt tttgtcgccc attgagaaca acggccattt
     3481 gatttcgggt atcagcggtg cttcaagccg caatctgaac acctttgatt ccgcgacaag
     3541 atttacctcg agataaattc ccgcttcaag ataaaatccg cctgtaagcg tccggtatac
     3601 cgtactgtat cggtgatccg gtttgacaac gtgatatttt gccagaccgt atatatcaag
     3661 atatgcgcct acctggacct caacgccgac ctcgcccagt cgccttaaac ctgtgaacga
     3721 aacggtaagc tgtccggaaa cgcccatctt gacgccgaca tatccgaata ccgtaagatc
     3781 aaagctgtac ctgtcgccct cgaccagctt gtttttgtac atactgaact ctttatcctt
     3841 tgagttgccg tgtgtaccta cctgcgttgc gtcaagtatc gagaacttac ttcctatacc
     3901 gcctgcaaag ctcatcttta caacaaggct gagttcaaag ttaagctgga acagcgacag
     3961 cggtttaaat gccgtgaaga acggtatacg gcatatttct atctctccgc ccttcttgtt
     4021 aagcatatcc ttgacatcct ttacaagacc tgcggtatcg ttcttctcat cactgccaag
     4081 catctttgcg acttcgctcg tgatattcct gtagctgttt gaacctaccg agcagacaag
     4141 aactttaagt tcaatatccg tctgtgtgta gatattagcc gcatagttaa attccatctc
     4201 gggtatatca aacaannnnn nnnnncctgg aatgaaacat taagatattc gcttactgta
     4261 aatgttgcat ctaccttgag ctttttcttg atggtagtct tatagttcag ctcaaatgta
     4321 atgaccgacc agtaatccgg gttaacggta gtaaagttat cgttttctgc ttcaccgacc
     4381 tttacggata cggaaagacc gatagcaaga cttgcggcga tactcttgag cgtatcctct
     4441 ttaggacgtg tatagtccga gaaatccttt aatctgtcat cgtccgaagc ggcggttact
     4501 gtatcggtaa catcttctgc aagcagtgcg gcaagcacaa ggttcagctg ctgtacgccc
     4561 tcgctgttct tcacttcttc cgctatcgcc tcggtatcta tctgttcttc aaggaatttg
     4621 ttttttgcag gctcgacaaa ataaacatcg acattgtcat aaacatcttc cgcttcgcaa
     4681 tcggctgtct gaatgtagta aatgtttccg tcctttattg tatctgtaat ttttacaaac
     4741 aggcagtttt cggtaaaatt ctttgtgccg ttgtcaatgc aaagcacatc gcccacagca
     4801 agagaatttt cttcaaaata atccgcctga accatatact cattggtttt atccgtagcg
     4861 aacagcttgg ctttatcgag atacttgata ttgtccctga cctcgattat attagtctgc
     4921 tctttgtata tcttgaatgt atactcgttt accgtatcgt caaggccctt gaatgtaact
     4981 ccctcgcctg ctgtcatacg gtacatacag ccggctgtgt atgcggtttc gggaacgagt
     5041 gtatattcat ctttctcgtt tgatgttaca acgaaatttt ccggagtatc acccgtatac
     5101 gcaaataagg tgatgtactc ccatacatta tttgccgtta tttcttcgtc acatataagc
     5161 gtgatcggct gatattccgc acagtcctcc ac
//
LOCUS       DAWAOG010000077         5189 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2085_length_5189_cov_1.945851, whole genome shotgun sequence.
ACCESSION   DAWAOG010000077 DAWAOG010000000
VERSION     DAWAOG010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2085_length_5189_cov_1.945851"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2178..2187
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2416..2425
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1620 a         1008 c         1254 g         1287 t
ORIGIN      
        1 gtgacactgt attgaactga tagcgtcaag cggaaaggag aacggatatg ccggaatttt
       61 tactttattt actgctgata gcagccttta tcttttctat atttacctca gtcaatgtta
      121 aaagcacatt cagaaaatac aacagaatgc cgagctcaag aggagtgtcg gcggcaacga
      181 tagcacgaca gatccttgac agcaacggac tttataatgt acaggttaca agagtatcgg
      241 gtgagcttac cgaccattac gacccgagga caaatacagt agcactttct gcgccggttt
      301 acgacagcat ttctgtaggc gctataggtg ttgcggctca tgaggtcggt catgcgatcc
      361 agtacgccaa gaattatacg cctataaaga tacgaatggc gatactgcct gtggctcagt
      421 tcgggtcatc ggcgtggata ctgtttttta tactcggtat agttttcaat atttcactgc
      481 tcagaggcgt aggcatcgga ctttttgccg caatagtgct gttccagctt ataacgcttc
      541 ctgttgagtt caacgcaagc agaagagcgc ttgctactat caaggatcag ggcatactgt
      601 tcgctgaaga gtacaaaggc gcaaagaaaa cgcttacagc tgctgctatg acctatgtag
      661 cgtcacttgc tgtagcactg ttacagctgt taagacttat agcatgttca agaagagatt
      721 gatttggaga gatgaatgaa aactgccaga cagataacac tcgacctgct tatccgtatg
      781 gatacgcagg gtgcatactc gaatataata cttgatcacg catttacatt aagtgatgtt
      841 gacaagcgtg ataaggcgtt ttctgcggcg ctgttttatg gagtgctgga acgcagaatg
      901 acgctcgact atcttatacg ctactactcg ggcatagaat ttgataaaat caaaacagcg
      961 gtagtcgaaa tactccgtat ggggttttat cagctgttgt ttatgaattc tgtcccggac
     1021 agtgcggctg taaacgagtc ggttgaatta tgtgaatatt gcagatgcac acgcgcaaaa
     1081 ggctatgtaa acgcaatact gcgtagcttt ctgcgcaatg aaaagcagat agattacggc
     1141 aatctcaaag acgaggctaa gctgtctata gaatattcct gtcccaaatg gcttataaaa
     1201 aagtggaaaa acgagcttgg cgagcaaagg gcgatgcaga tgatacattc aggcttcggc
     1261 agacctccga tgtacgccag agttaatacg gtcagatatt ctgttgacga tgtgatatcg
     1321 gagcttgcaa aagacggcat aacggcagta aagaatatgc ttatagaaaa ttgtatcgag
     1381 ctttccgcca ctaaggggat agagcagagc gttgcacata aaaagggaat gctccatata
     1441 caggatattt cttcacagat atgctgtaaa atagccgctc cggtatttaa cgaaacagta
     1501 gtggatcttt gcagtgcacc cggcggaaaa acatttacct gtgcagagat gatgaacgac
     1561 aggggcagga tttacagcta tgatctgtat gacggcaagg tttcggttat tgccaatacc
     1621 gcaaagaggc tcggacttac cataatcaca gccgctgaaa atgatgctgc taaatttaat
     1681 cccgacatac cgaaagcgga caaggttatt tgtgatgtgc cgtgctccgg gcttggagtg
     1741 atcagaagga aacccgagat aaagtataag cctatgaaat tacttgaaac gcttccggat
     1801 actcagagaa aaataataaa taatgccgca gaatatgtaa agccgggcgg aacgttgata
     1861 tattcgacct gcactgtaag taaagcggag aatgacgaca ttgtcgatga gtttttggcg
     1921 gaacactgtg actttgtccc ggttgtaatt cctcttggcg taaaaggact tgaagaaagc
     1981 tataaaagaa cgatgctacc atgcgatata aacggtgacg gcttcttcac ggcaactctc
     2041 agaaaggtaa agtgacatac atcatatgga aaaaatcgat attctttcgc tctcacaaga
     2101 agaactggaa gaaaaaatac tcgatatagg cgaaaagaaa ttcagagcta aacagatata
     2161 tgactggcta cataacgnnn nnnnnnntat ctttatggtt tgtctgacgg agaaaaagtt
     2221 gaaaccgtgc ttatggaata taagcacgga aacagcatat gtatttcaac tcaggtcggc
     2281 tgtaaaatgg gctgtaagtt ctgtgcgtct acaaaggcgg gatttgtacg aaacctgctt
     2341 ccgagcgaga tgcttttgca gatctacgaa agtgaaaagg acagcggacg caagataaat
     2401 catgtggtgc ttatgnnnnn nnnnnggtga gccgcttgac aactttgaca acgttgtaaa
     2461 gtttctgaag cttttatcgg caaaggatga tatgagtctt cgtcatgttt cggtgtcgac
     2521 ctgcgggctt gtagacagaa tatatgagct tgcggatatg aagctcggca ttacgctttc
     2581 cgtttctctc catgcaccga caaacgagct gagaagctcg gttatgcccg taaacaacag
     2641 attttcaata gaagaactta tgaaagcgtg caagtattat ttcaatacta caggacgcag
     2701 gataagttat gagtttgcat tgatagacgg cgtgaatgac acacgcaagt cggcagatga
     2761 gcttcttaag ctgcttaagg ggcagaactg tcatgtcaat cttattccgg taaacgaaat
     2821 caaagaaggc gtattcagac gcagtgcttc tgtagaaaag ttcagacaga tgctgatcga
     2881 cggcggactt aacgctactg tcagaaggac gctcggtgcg gatataagtg ccgcctgcgg
     2941 acaattaaga cgggataaca agtaaagtga tatcaaggga gaactattat atatgaaagc
     3001 ttacgcaaag accgatatcg gtaattgtcg tgatgaaaat caggataact attggatagg
     3061 cgaacttgca aacgatgctg tcgctgtaat tctgtgtgac ggaatgggag gacagaatgc
     3121 cggcggctac gccagtctga ctacagttga ctttatcaag gacagactga taaaggggtt
     3181 cagaaaggac ataacccgaa accagataag aaatctgctt ataacatctg ttactgcggc
     3241 aaacagtgtg gtatatgata tggcaaagac agatcccgat aaagcgggaa tgggaactac
     3301 ctgtgtagcc gggatcatct tcaatgaaag ggcttatatt atcaatgtcg gtgacagcag
     3361 gtgttatttt ttctttgacg ataatatgca gcaggtcaca aaggatcata catatatcag
     3421 aaagcttgta gaaaaaggca taattactta cgaggagagt aaaacgcatc ccgacagaaa
     3481 ccgaattaca aaggcagtcg gacctcaacc ttttattact cccgattatt ttgagcttga
     3541 tgttaaaaag gacgatatgc ttcttttctg ctcggacgga ctgagctcct atgccgagga
     3601 tactgagatt gctaacgtaa tagcagcttc tcctgttgct aagtgctgtg atcagcttat
     3661 agattatgtc aataaacacg gcggacgcga caacgtgacc gtagccgtgg tttcaatata
     3721 aaacgactta ccgtatgggt cgcacattaa actgatacgg agaaacggtg gattttatgg
     3781 ataacaatat ggacaatatg ataggcagac gccttgacgg tcgttatgag ctgcttgagc
     3841 ttatcggaac gggtggaatg gctgatatct acaaggcgag ggatattacc gaggacagaa
     3901 tagttgccgt aaagatcttg aagaacgaat ttgcaggcag cgaggacttc ctcagacgtt
     3961 tcagaaacga atccaaggcg atagctctgc tttcacaccc gaacattgta aagatatacg
     4021 atgtcggctt caatgagcag atacagttta tcgtaatgga gtacatagac ggaatatctc
     4081 ttgccgagta catcaagctt cagggtgttc tcaaatggaa agatgcggta tattttaccg
     4141 tacagatatt aagagcttta cagcacgctc atgacagagg tatagttcac cgtgacatca
     4201 agtcgcagaa tgttatgatg cttcccgacg gtacaataaa agtcatggat ttcggtatag
     4261 cacgttttaa ccgggaaacc gacaagacgt tatcagaaaa ggctatcggt tctgttcact
     4321 atatcagccc tgagcaggca agaggcgaga tgaccgatga aaagagcgat atctattcag
     4381 taggcgtaat gctctatgaa atgctgacag gacagaagcc ttttgacggt gaaacacctg
     4441 tagctatcgc gctccagcat atgcagactc cgcctaagcc gccgcgtgag ataaattctt
     4501 ctatacctga aggacttgaa gagatcacgc ttaaggctat gcagaaagaa ccctcaatgc
     4561 gttatcagac cgcaggcgag atgataaacg atatagaaga gttcaagaaa gaccccagca
     4621 tagtgtttga atacaaatac ttctcggctg acggttcaac aaagtacttt gataaagtgt
     4681 ccgatcccaa tgctcccgag cagaatgcgg caaatgatac actatccgaa agcgaagagg
     4741 aagaggataa cgatctgtac gacgatgatg aagatgatga agaggtagaa gaaagacgtt
     4801 cgccgctttt acctatactt tttgcagtag cgtctgtttt cgtaataatg accgcatggc
     4861 tgatattctc gatcgttacg aaaaaccttg gaactaccag cggtacaaca gatgaaatga
     4921 ccatgcctaa catcatcggt atgacactag atgacgtaaa ggagaaatat cccgatctta
     4981 atcttgatat cgacagacag tattctaccg attacaagga aaacgaagta atggatcaga
     5041 aaactcccgc aggaagaact ataaagaaga gcacaatagt caacatagtt atcagtaacg
     5101 gtccacagaa ggtagaggtt gatgattatg caaacctttc tgctcaggat gcacagataa
     5161 agctggagaa aaagggactt aaaccaaaa
//
LOCUS       DAWAOG010000078         5071 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2145_length_5071_cov_1.859450, whole genome shotgun sequence.
ACCESSION   DAWAOG010000078 DAWAOG010000000
VERSION     DAWAOG010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2145_length_5071_cov_1.859450"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3368..3377
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4161..4170
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1379 a         1138 c         1069 g         1465 t
ORIGIN      
        1 tcgttagacc atgcaaagag gaagagcttg cacttgagcc aaaggagaca atgcatttcg
       61 ggcagtaata taataccgca attccggatg ggattgcggt atttttgtat ttacaccctc
      121 ttttcgagca tcacaagcat aacatcgggt atcccgttga acacggtcgg tacgatgcct
      181 atgtagacct tagagtactg tacctctcct gaacagtcct tttacaaaaa ctattctcgc
      241 tcctgcccgc cgccacacgg cttatgccat acaggcaaaa taatatctat tcttttttaa
      301 ggctacaaca aagaaccgtc gcaatcacac catgcgacgg ttcaactttt tctgtcatta
      361 ctgttacaac aatttcggtt tcatactcat cgcttggtcg aaaccttgca gttcgaccat
      421 gcgctgtact gagtgttctt gccaatagtc ttgtatgtcc ttattctgaa agcatagctc
      481 ttgccggacg taagaccgtt caccttgcag gataatgtga tattactgcc gatacgcttc
      541 atatcagtcc acttcttggc agaagcatta taatactgta gcacatatcc tgtaactttg
      601 ctgttcttgc tccattcaag cgtaatactg ttctttgtcc tttcggaagc cttaaagccg
      661 ctcacgcccg taggatttgt ccttactgtg caggtagcgg aaacagtacc gtatcttgcc
      721 tgcttgtcag ctatgcggta agccttcaca cggaacttat aaacagtgct ggaagaaagc
      781 cctgttactt tataggaagc agttgcttta tttgttatct tggtaactct tacccatttg
      841 ttgttcttat actgctcaac tatatagcct tgtgccgtcc tgtgcttatc ccaagtaagc
      901 gttacagttg aagctgtctt ggcgcttgcc ttaaggttct ttacggcgta ggggtttgtt
      961 gtggcattta tatacatcca tttactcgac agtgtacctt tataggcttt tatgcggaat
     1021 ttataggtcg ttgccgttga aaggctcttt actgtgtagg ataccgtggt attctttgtg
     1081 gtcttggcta cctgaaccca tttgttattc ttgtactgct gtattatata gcctgtagcg
     1141 gcgctgtttt tgttccagcc gagcgttatc gatgtggttg tcgctgactt tgacttcact
     1201 ccgctcacag cgctgacagc gatttttgtc tttgcggtca gcgtagacga ataaccgctg
     1261 taggcatatg tgcggcctga tttcttatat gcctttatac ggaatttata cgcggtgtcg
     1321 ggctgaagtc cttttatcgt acagcttgtg ttgctcttgc ccgtaacctt tgcggctctg
     1381 acccatttct tgccgttgta tttctcaatt atatatcctg cggcttcatc actgcttgac
     1441 catttaagcg tcaccgagga cgaggtcttg ctcttcacgg aaaggtctgt caccgcgaac
     1501 ttattcttta ctgtaaactt gaatacctgc ttcagcgtcg gattgtatcg aggatagaat
     1561 gttaccgttg cgctgcccgc cttcttcatc ttaagcgtat tatccgaaac gcttacaatt
     1621 gcaggatcag acgattttgc aacgacttcg gaatcaatat ccataccgta aaaaaagcta
     1681 attgtacttc cggcaaaata agtataggta ggcaatgtca tataatacaa tccgtcatta
     1741 tgttgccaat acacagcaaa ctgggtagct accttctttg tcgtggtttt gccgcagaca
     1801 gtgcatttaa gctttgctgt gccgtttgtt gttccgttcc acttgtactt atgaccgttt
     1861 acaatagctg ttttcttgtt agatgcggga cggctcatgt ttatctcgta gaaattgacc
     1921 ttttcgttat tccatgtgta ccatacaagc ttgcctgagc ttacgatcgg aacgcagtcg
     1981 gatatctcgc ctgtcatgga atatatctta ccggtcttat ttccgtacgc gtcaaggaaa
     2041 gtatagtata cctttgtgtc cttcgtccag agcagaatat atttgtccgt tgcgactttg
     2101 acaagatgcg gagtgccgat atcggtgctc ttttcgcttg ttatcatctt taccgttgcg
     2161 gtcgttccgt tctttttaag cgttgaaaca aagatattcg acacactgtt gctgtcgttg
     2221 aagtttatct ttgttcccac ggtaatatat gccttgcttg acacctcaaa gccgcctacc
     2281 gtaacgccgg tgtagttatc tcctgtcgca ccgggaatat ccagcataga ataatatttg
     2341 cacggagaat cgaaatagtc cgcctgaaac ttaccttttg acacatcagc ccggtattta
     2401 ataagagcaa gacttctcgg atacgcatca ccatggtcga gagtaactat tttattgccg
     2461 tctaccgcga tgaactggtt gaatgagtgg ctgacatatc cgacgttgac atccataatg
     2521 tcataaaatg catcagtgat cttcattgcc gaggtgtcga cctggatggt aacattcgcc
     2581 tggtgatgta agccatcact tgttgcatac atttcatgac aggtatgcag aataagatac
     2641 ttgccgacct gtgcaattct gcaagtacca ccgtcaaagg gaatgtatgt attgcagttg
     2701 ctgagtccca ctgacgaaag acgcttccag ttcttgtcgt acttagttat tctgtagcat
     2761 tccacggtat tgctctgttt aaggttctcc tgaccgctta caatatagta gttggcagag
     2821 gtttcataga atgcaccgaa tatcggcaac tcctgtttta tctttttcgt gcctgtaaga
     2881 ttgaacgact tgtcgtaata ttcaacaagc acaccggatt ctcctaaatt ctgtacacgc
     2941 atatatccgt ttgtgcggtt gcacagataa gactcggtag gagtccccca gtaaaagctg
     3001 taattgttac tgtaagtatt agagccatta tacaccgtac aagtcgtctt gacggcaaat
     3061 gcgcagacag tcagcgccgt acagcatatc atggcaaaca gtgaaaccgt tacatatttt
     3121 accactctta aagttatgtt tttcttcagc atatttgtac cctcccaaaa tgccgtttta
     3181 tcgctttaat tatacaaaga aatttaatat ttgtcaataa gaaataaacg caggatatct
     3241 gtccgccaac cgcctgctat atgatgcacc aaatataatt accatcattt ttactaactt
     3301 tagctctcaa ttcttgactt ttttgtagaa ctactgtata ataaaatcaa aatacaaacc
     3361 acataatnnn nnnnnnncgt atgaaaaaga taatcgcgac agctgtagca acaatagctg
     3421 cggcattact gctctgcttt tctgcgggag cgtttgacaa taacgactac ggcggtggag
     3481 gcgattacga cttcggaggc ggatacgatt acggaggtaa cgactacggg ggtaatgact
     3541 acgacagcag tagctacagc ggagatgccg accccatttc agttataatc ggaataggca
     3601 tagttgttgt cattattatt atatccacga tcggcggaaa caaaaagaaa tcttccggcg
     3661 gcacaacacg tccgtcatca atgaacggtg caaatgttgt tcttccaaac aggactgaac
     3721 agatagaagg aattatcaaa catggagatc cgaacttctc ggcttccgac tttatcactt
     3781 tcacgaaaca agtatacata gatatcgaaa atgcatggtg caaacgtgat cttaccccgg
     3841 ttcgccccgt aatgcacgaa aatctctaca acactacaaa caagcaggta caggctaaga
     3901 tcgaccaggg catagtatac cactatgaga gcatcgctat agatactgcc tatcttacct
     3961 catatgaacg tgattcacag tttgaatatg taacagccta cctcacttca agaatgattg
     4021 attatcaggt agacgaaaag accggcaaga tcataagagg tgataagact actcgctggg
     4081 tacagaaata taaaatgaag tttgtacgtt cgctcggcct tcagacaaag acagaaacgt
     4141 cttattgagc gtgtgaagtc nnnnnnnnnn acaaagacag aaaccggcgc aatgacaggt
     4201 cacaactgtc cgaactgcgg agcaccgctt gagatctctt cttcaggctt atgtgaatac
     4261 tgcggctctg ttgttacaac aggtcagtac acatgggtac ttaccgactt tgcaggtatc
     4321 aggaacgata ctgtagatga cggcataaag ggagtataat gtggatataa catcgttcat
     4381 tctttcaata ttaagctggc ttgtcccgct tatagtcata ggcattatcg ttttagtaat
     4441 agtagctaaa atacggaatg cgacacagaa atatctcggt atgtccccgt cacaggcaat
     4501 ggaacttgta aataagggta tgaaggaaga aacgactacg ccaaagggaa taacagccct
     4561 cagcgaaatg tacggtcctg ctatcacccg tgactttccc gagatcggat acaagggcat
     4621 ggaagtcaag gcaagaaatg caatagtcgc aattctcaat gccattatga acaaaaatgc
     4681 cgaatcgctt aaggcgagcg aatatacaca ggatcttata aataaggtca acaaccgtat
     4741 aaccaccctt aatgcgaaca acgaaaaaga gattttcaat aacataaaaa ttcaccgttg
     4801 cggaattgca agctatcaga acagaagcaa ggaggcaacg gcaaggtttg aaatttctct
     4861 tcaatatgaa tacgcaaagg tatccgagga taaagcggat acggcaaaac ccgcacttac
     4921 gcagaccgct tacagcgtaa cgctggctta caagcaggat ctgcacgaac agacaacaag
     4981 cattgtgtat tcaagcaact gtcccaactg cggtgctccg atcgatgctt ccgcaaaggg
     5041 aaagaagtgt ccgtactgcg gaagcggcat t
//
LOCUS       DAWAOG010000079         5024 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2168_length_5024_cov_2.185349, whole genome shotgun sequence.
ACCESSION   DAWAOG010000079 DAWAOG010000000
VERSION     DAWAOG010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2168_length_5024_cov_2.185349"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    451..460
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1561 a          933 c         1276 g         1244 t
ORIGIN      
        1 gtgctacact tgacgagggc gttttatctt cgatcggcgt tgattcaata accgaaagca
       61 gtgtgtctga taccgaagaa acgagcggca atattgacga tagctcagaa gttgagtcgg
      121 ataatgtatc tgaaaattca tctgatgaaa atatttcaaa ttccggtgat acatcttccg
      181 atgcttccga caacacctcc gaaccggaag cttcatcaag ggcagatggt acatcaacac
      241 ctatcggcac agaacagcct gttgccacag agaagcctgt cgatacaaaa gagcctgcgg
      301 acacaacaaa ggtcgtttct tctgcttctt cagcttcaga aaagaatgat acgcagagca
      361 agcccgcagt ttcgcaaagc aacttcactg ttcagtggaa gaagtcgagc gactgggaag
      421 agggcggcaa gaagtgcggc ggttatgagg nnnnnnnnnn acggcgatac ggtaaacagc
      481 tggacagcgg aaataacagt gcctgacggc atgacgcttt cttcaagctg gaacggaata
      541 ttcagcattt cagataataa aatgacggtt aagaatgaga gctacaacgg tactatagaa
      601 aagggaaaga atgtttcgtt cggctttaac tatgcggcta acgcttctat gaatgagggc
      661 aaagtgacag tgaacggaag ctctgccggc acaagttcgg caaacagcgg cggaaataat
      721 aacaacaata ataatacggc aacgactcga aagcctgctt caacagttcc tcccgcgccg
      781 agcgacccta aaggtacaac tcctgtttct cagcacggtc agctttcggt gaagaacgga
      841 cagcttgctg acaagaacgg caagggctat cagctcaggg gaatgagcac acacggactt
      901 acatggttcc ccgaatttgt gaacgagagt gcattcagga cgctccgtga cgactggaat
      961 acaaatgtcg tgcgtctggc tatgtatgtt gacgaatggg gcaacggaca gtgctatatg
     1021 ggtaacaaga gcggaagcct tgagcttctc gagcagggcg ttgacatatg tatcaagctt
     1081 gatatgtatg ttatcatcga ctggcacgtt cttaatccgg gcgatccgtc aaagtatacc
     1141 gatgaggcga tatcgttctt tgaaacggtg tcaaagagat atgcaaaata tccgaatgtt
     1201 atctatgaga tatgtaacga gccgaacggc ggagtatcat ggagtgacaa cataaagcct
     1261 tatgcagaga aggtgatacc tgttatccgc aagaatgcgc ccgactctgt aattattgtc
     1321 ggcacaccta catggagtca ggagatcgac aagccgcttt ccgacccgct tgacttcaag
     1381 aatgtaatgt atgcgttcca cttctatgcg gcaactcacg cggggcttcg ctcaaatgta
     1441 gaaaactgcg tggcacaggg actgcctgta ttcgtgtcgg aatttggtac ttgtgacgca
     1501 tcgggcggcg gagcaaacga ctttaatgaa accgaaaagt ggctgagtta ttttgataat
     1561 aatggtatca gctactgcaa ctggagcttg tgcaacaagg acgaaacctg ctcggtactg
     1621 cgacccggga catcggcaaa cggtaactgg agcgagagcg atctcactga aaacggcaaa
     1681 tggatacaca actggctgaa gaaacactaa gcggcggtgc ccatggggca cggcaaattc
     1741 cgacctttgg aaatgtcggt agttggcgtg actttgcaga acggtcgggg tttgacgatg
     1801 gctgaaaata agctgtcgaa atacttgttt gtttattccc gccgttaaac aaacgtgaat
     1861 acacagcaac cgtatttaaa ggcggaaatg cgagccgagg cactcggctt ccgacctttg
     1921 gaaatgtcgg tagttggcgt gactttgtgg tacggtcggg agttaatgaa aacaaggaga
     1981 taatatgctg aacgatatta aatggttatt tttcgatgtc ggttcaacac ttgttgacga
     2041 aaccgagtgt tataatcatc gcatacgaga tgctattgcg ggaacagata ttacctttga
     2101 ggagtttaac gaaaaacgtt tgtatttttc aaagcaaaat ctgaaaggcg atattgaagc
     2161 actgagatat ttcggacttg caaaaactcc ttggcatcat gaagatgaaa agctctataa
     2221 agattccgaa aacgtactca aaatcctatg tgaaaaagga taccgtatag gtgtaattgc
     2281 caaccagtca gcaggcactg aaaaacgact tgaaagctgc ggcttgatga aatatataca
     2341 gcttgttgta gcttctgccg aagaaggagt tgccaaacct gataaaagaa tattcgatat
     2401 agcactaaag cgagcaggat gcagtccgtc cgaagcggta atgatcggtg acagagtgga
     2461 taatgatata attcccgcta atgaaatggg aatgatgacg atatgggtga ggcaaggctt
     2521 cggcaaatat tggacgattt ctgacgagag cgaaaaggct gattttgctg tggatagctt
     2581 gtcggagctt ttggacattt tttgataagg cttaggcgga aatattgcaa aagtcttaag
     2641 cactatgaat gaaagaaagg ttgtgtacta tgtttagcaa attaaaacat cggcttgaca
     2701 ggaaaagcaa aagtgtggtt gcgctgttgg caatggcgat actgctctgt gggtttacgg
     2761 ggtgcgaaag aagtactatt cctgatggtt ctgagatatc gtacagcgaa acgggttttg
     2821 acattgaacg gatacgaaaa aatgtaattg ttgacggaca agcgatcgag atacccataa
     2881 agctgaaaga actgcctaag gattggacat atgaaacttc tgctaatcga tttgcggagg
     2941 atagatgtca cgttgaagta aaggacaaaa atggcgcttt gctttttagc tgcggagcga
     3001 aaaactacaa agagtccaaa aagggtgaca gtaggatttt tgatgctcct tttgagaatg
     3061 acaaatgttc ggttgacggc ataactccat ttaaaacaac tagagaagaa atcgtcgaaa
     3121 ggtatggtga agcaacaagt ataaaaaatt ttaacagtaa aaaatatgaa ggctggcagg
     3181 aatatagata tggttctctc gagggaaaaa cctcgaatga ttggcacggt aaatgtctta
     3241 ttgttacgtt taacgagaaa ggcattgtcg attgggttac cattgtttat acagactgaa
     3301 aatgacgcct aagaaaagta tataaacgac tgggcaagct ttgaacgttc tgtaatgccg
     3361 gcaggcagag cagaaggaag agccgagggc agatcagaat tattatctga actgataaag
     3421 cagggtgtaa taaccgaaga acaggcaaag aactacagaa actaacaaaa taaactccgt
     3481 tcagacttat aaatgcagaa acagaattta aaacaaccat tataaaaatc cgtgtcaggc
     3541 gacacggatt ttttctgtta tagctgtttt aatatgtgca agaccgtttg caataaggca
     3601 ctgacccggg taatagaggg caaggcagaa attcgagaac gggaaatcta tttttgagaa
     3661 gtacgccatc aggagcatta tatcggatat aaggaacagc acgcttccga gtgcgagtac
     3721 aaggttgaat ctgttcttta tgcggataaa gttcattatt gctttgcctg tcataatgca
     3781 gattataaca gcgtatgaaa tgccgatgat tttcataagc gacgagccga agtcaagaag
     3841 cggtgagaac atcataaaca gcactatggg gataaatatt ataattgcgg ggataaagtc
     3901 ggtgggtcgg ggcttttgca tcagcgaata tgctatgaaa taacagatat gacctaaggc
     3961 gaaaatcgcc gcacctgcga tgaaatttag gtttatcact atatccgcgc tcattgcgaa
     4021 gaatacacca acagtcagag cgatcgatgg ctgtttcttg tcggggaagt atttcagggc
     4081 atatatcagg tttactgcgg cggcaagcat aaagtaaacg cttgcggtta ctttcagcgg
     4141 gtagagatga gtaattatca gagctacatc gcaggcgagc gataagccga gaaagattat
     4201 gttgagcggt aaaacgagtt ttttcatata tctgtttcct ttcgggcgat ttgattaaat
     4261 tatacactgt ttttatgaaa ttttcaatag attaaatata aaatacggtt aaatattatc
     4321 gggaatatgt gccggagcgg ttacagtaag ttaacttaaa atgctgtaac ctgttacatg
     4381 gaggtgctga taatttcgtg tgaaaacggc gtaaaattat cggaaatctg tagacaatga
     4441 cggtaatttg tgatacaata ataataacgc ctgtcgcaga ggtttttgtt ttgcaaaaac
     4501 aagtaaacga ttacctgaat ttcggtgtgt accatttggt atgtaccgcg gttcatatga
     4561 tgcggcggaa ttttgacaga cttataaagc agagaacaaa ctgcacagaa aggaattttt
     4621 gctatgaaga acatacccga tgtaaagctc ggaattgcgg cagtaagccg tgactgtttc
     4681 cctatgagtc tttcggcttc aagatgtgag gctgtggtaa aggcatgtaa ggagatcggt
     4741 gttgacgtat tcaaatgtcc caccactatt gaaagcgaaa cacatatgat gcaggctctt
     4801 gaggagctta agggcgcagg ctgcaacgca cttgttgtat acctcggcaa cttcggtcct
     4861 gagtcggctg aaacactgct tgcaaagtac tttgacggtc ctgtaatgtt cgttgctgcg
     4921 gcagaggaaa cacaggataa cttagtaggc ggcagaggcg acgcttactg cggcgtgctg
     4981 aatgcaagct acaacttagc gctcagaaat atcaaggcat acat
//
LOCUS       DAWAOG010000080         5021 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2169_length_5021_cov_2.791583, whole genome shotgun sequence.
ACCESSION   DAWAOG010000080 DAWAOG010000000
VERSION     DAWAOG010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2169_length_5021_cov_2.791583"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1488..1497
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1558 a         1130 c         1175 g         1148 t
ORIGIN      
        1 cccttctgct gtccgctata ctttatgaaa gcgttttcgg aaaacgattt gagatatatg
       61 attcggcgca cctgcccgat cttaaggatt ataaggggct cataagcgat cctgttacat
      121 ttccgtcaaa gcgcggttat aagtacaccg ggttttatta tcacgatgaa agctatgaca
      181 gcttcagggg gcttatagtt ttctcgcacg gcatatttga cggacatctc agctatctgc
      241 ccgaaatagc gtattttgca agacggggat acaagatatt tgcctttgac aacagcggca
      301 gtcatttaag cggcggaaaa tcgcttcgcg ggcttcctca gtcggcggag gatcttgacg
      361 cggctctgag ttatgttaca aagacaaata cgcttccgct gtatctgttc ggtcacagct
      421 ggggcggata tgcggtatcc gctgtgcatt gctatcatct ttatgatata aaggcggtat
      481 tcgtacagag cggctttaac agcacgagcg aaatggtgct tgaagagggc tctgccatga
      541 tgggcaggtg gatatatgtc ctgaaagcct acataaagct gtacgagcgt atgaagtacg
      601 gcaaggccgc aggatacaca gcacacgcag gaatagcaaa agcaaccgca aacggcacta
      661 agttccttat actgcacagc acggacgata agacgataag ccttaaaaac agcgtgcttg
      721 ccaatgttga gaaaaacgat aatatcactc ttataaccga aaagaacaag ggccacaact
      781 cgcttgacag cgacagggcg ataaagcaca aggcacagct tgacacgctt tttgaaacga
      841 cctatccgaa aaatcaaagg actaacaaga atagaagaga actgttcagc aaaaaggtcg
      901 ataagcaaat ctactacgag cttgacggca agctgatgaa gctgatagcc gatttttacg
      961 aaacggctca gagtgggaag ggtttcagca gtgcgggggt gcaggggaaa aagggaagga
     1021 aaaaataacg caccgcttac gataaacata acaaaataat atcacaactt tgcgagcgac
     1081 ggttttatta accgccgctc gttttttgtc tgttacacag ccgttaccct ctttttaact
     1141 taacttataa atgcaaattc tgttagtttc atgaaacgtt acattaagta actaaaaaac
     1201 aggctaactg aacattcaag ccgttattta agtttcgctt tggcgaaatt ttgtgtaaat
     1261 actacttgaa attgtattaa atgcacataa atcactttta actgttgtga aattcacaga
     1321 aacaatcaac tttgttaagt aaatttagtt aaacctgttg actttaaatg aagcggcatg
     1381 atattatgag gttaagctaa gaaaccaggc ggctatatca gaataatagc agaaggagtg
     1441 aaatcatgac aggttttaag aaaagactgg caagcacggc gcttgccnnn nnnnnnncgg
     1501 caatgacgct taccgcttta cctgtatccg tgtttttaat aagcacagat gctgaggcag
     1561 gagttccgaa cggatttgcg tatacgcagg gaacaagatt catgcttgac ggaagcccgt
     1621 tctactatgc aggtacaaat tgctactacc taacgttcaa atcgcaggag gcggtagaca
     1681 gcgtattcaa agacgcagag gcgatggggt tgaaagttat cagagtctgg ggcaaccttg
     1741 atgtaggtgt caagacaggt acgacagaca gcgaaggaaa gccggtattt acaaacaaca
     1801 atgacggccc gggcgaaaag gacgggatct attttcagta tttcgacaag gatctgggaa
     1861 aaccggtgat aaacgaaggc aaggacggct tgcagaagct cgactacgct ttatatcagg
     1921 ctgaaaagca cggtataaag ctgctcatca cctttactaa ctactgggac gctttcggcg
     1981 gtatgggtca gtatataaag tgggcgcagg agcttggcat aaccggactt aaaaaagacg
     2041 acttctacac aaacgaaacg ctcaagggct ggtataagga ttatgtcagc ggtctgctga
     2101 accatacgaa cccgtatacc ggcagaaagc tgaaggatga gccttccgta tttgcgtggg
     2161 aacttgcaaa cgagccgaga tgcaataccg acgctcagtg taaggacaat atactttata
     2221 actgggctaa ggaaatgagc gaatatgtaa agagcgtcga cccgaatcat atggttgcgc
     2281 ttggcgacga aggcttttac aataaacctt acggatatta caacgaatat actacatcca
     2341 attatgcctt ttacggtgca gagggcgttg actttgaaaa gctgatgaca atcgacacgc
     2401 tcgacttcgg tactcctcat ctttatctcg atcagtgggg aatgaagcat acaggcacag
     2461 gacaggacga tctgctctgg tttaagatac acggcgaaac ctgtgcggag cttggcaaac
     2521 cggtcattct ggaagaattc ggtcttacaa acaggacgat ccgtgacagc gagtacgagc
     2581 agtggttcga ggtgcttgag ggcaatgtct acgaaacagt cgaatacgca ggcacaaact
     2641 actggatgat agcctcatac attgacggtg cgctttatcc cgactatgac cagtataccg
     2701 tttacggtcc ggagggaacg gatacagaaa gcacaagaca gcttatcata aagcacgccg
     2761 caaatatgac agcaaagaat agcgtcaaca caatagcaag cgacaagctg agctttgaca
     2821 gagccgacac taccgacctt acggtaacgg cagctatgaa ggagggtgta ataagcggta
     2881 taacccttga cggaaaagcg cttacagaag gcaaggactt cacgataagc aacaactccg
     2941 taactataaa ggcaagctat cttaaaacac tggagcttaa aagccatgtg ttcacactta
     3001 actgtacggc aggcagcagt ccgaagttca cagtaaacgt aaccgatgta tcgatccccg
     3061 caccgacgct tgacaaatat atcgctacag ttgacaagaa cctgcgtcac tataagcagg
     3121 tgaatgttgg ctacgacaag aaaacaagcg aattcagggg cataacggta aacggcaaat
     3181 atctcacggc aggtgtggat tataccgata acggcggctt agtcacattc tctgacagct
     3241 ttataaaatc tctcggcgag ggcaacgtaa cgcttgtgtt cgacttctat gagggtgcag
     3301 attgtgaatt ccagcttacc gtagccgata catcggcact ggaagatata gatacttttg
     3361 aaaactacac agacgattct cagctacgca gtgcatatac accgaataca aacggaaaca
     3421 acataacggt ttcgcttgtg gacagcacta acggcaaagc gatgcgcttt gaatacgatg
     3481 tcagcctgcc caacggatac agcggcgtaa accacacact ttcacagcgt aacgttgcac
     3541 agtacaaggg cgtcagcata acccttgccg gcgacaacag cggcaacacc tttacgcttc
     3601 agttcaaaga taagaacgac aactattttg aaaaagaatt cgctgtagat ttctacggtg
     3661 aaaaaacaat caagatacct tttgaagaat ttaacgctcc aagctggcaa tcttcctctg
     3721 ctacgcttga cacaacgggt atcgtacagg tcgcattcta tgcaggtcgc agcggtaacg
     3781 cggacacagg cgcttatata atcgatgatg tatacttcta taacgaggaa caggaaaccg
     3841 agggtgcacg ccttatcgac aagacaggca catttgacgg ctctgctcct gcggctgtac
     3901 ttacaaagct cgtgctgaac ggtcaggcta tatcaaagat aacctgcggt gacaaaacgc
     3961 ttaacagcaa taccgactac tcgtggaacg gcagtcaggt aacgctcaac acaccgttta
     4021 cacaggctct tgataacggc aaatacgaaa tagtatatca cttcagcgac ggcactacag
     4081 acacatttat tctcaccgtt atcaattctg cggttacaga agatattacg gttgataatg
     4141 tcacaaactt cacaagcaca aacgcaaccg attcctcgct cactcttgcg tgggacaaga
     4201 acaacaaggc agaaggctac actgtcgaaa tatacaaggg cggtaagtgg aacgagatat
     4261 acagcggagc ggacacatcc tgcaccgcaa aagacctcag ggcttcctcg acctatacct
     4321 tccgcataag agcatacaag acttcgggcg gcacaacata ttacagcgac tatgtaagac
     4381 ttgccgctaa aacagcggaa agctcaggcg tttcaaacgt taaatccttc agaaaaatct
     4441 cagcgacatc atccgagctt ataataggct gggcaaagaa caactccgca gaaggatata
     4501 tcgttgaaca gtacaagggc ggcaaatgga cggaagtgtt aagaacctgc acaaataaca
     4561 ctgtaggctt tatagcgggc gaccttaagg caggtacgac ctacaccttc cgcataagag
     4621 catacaagac ctcaggcgac acggtcatat acagcaacta tacaagactt gccgctgtga
     4681 caaacgcaaa ctaaaagcat aataacagca aacggagcat atcaggcaga tatgctccgt
     4741 tgtttcttta tccgaattat ctgaaaagcg tacattctcc gcttttcctg taatacgcta
     4801 tgcgttcctc tatcacctca tgggtagtgt tgtaatactc cgcaaggcaa tctatgcaga
     4861 aaaactcatc ggcgttgcgt gtgacaagct ttctgtaaat cgctatatca tcgccgccga
     4921 gcggtgcacc gcactttttg cacgtcttgt agtttgccat taaagcttta ctacctttgc
     4981 acggaacatc aggcagtcat gtgactcgat ctgtgccgca t
//
LOCUS       DAWAOG010000081         4950 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2217_length_4950_cov_2.028396, whole genome shotgun sequence.
ACCESSION   DAWAOG010000081 DAWAOG010000000
VERSION     DAWAOG010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2217_length_4950_cov_2.028396"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1344..1493
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2831..2840
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3260..3269
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1454 a         1099 c         1064 g         1163 t
ORIGIN      
        1 aaaaagaggt cccgcaagat ttgtatctct gcttacaccg ctggatctga tataccgttt
       61 attcaataca cccggcgcat tgagcgaaaa tgcagacctg ctggatagcc ttgactatac
      121 tgaaccggac aaaaagcttg atgaaatacg aagcaactcg ctgaactggc tgaaaaatgc
      181 aatattcagc aaaaaaaccg gtgaaagcaa tactgtattt gattacgaaa gtaaatcacg
      241 caataataaa gcagaaagga actaaaatgt cacattacga ttatcttatt gtcggtgcgg
      301 gactgtttgg tgcggtcttt gcatacgaag caaacaagag aggaaagaag tgccttgtta
      361 tcgacaagcg caaccacatt ggaggcaata tctataccga aaaggtggag gatatcaatg
      421 tacacaagta cggcgcacat attttccata cctcggataa ggctatctgg gagtatgtaa
      481 atcagtttgc ggagttcaac aactatatca actctcccgt tgcgtcatac aaggacgagc
      541 tttataacct gccgttcaat atgaacacat tcagcaagat gtggggtatc aagactcccg
      601 ctgaggctaa ggcaattata gcaaagcaga tcgaagacct cggcataaca gagccgaaaa
      661 acctcgagga acaggcactg aagcttgtcg gaacagatgt ttacgaaaag ctcatcaagg
      721 gctataccga aaagcagtgg ggcagaccct gcacagagct gccgtcattc ataatcaagc
      781 gtcttccgct cagatttata tatgacaaca actacttcaa cgaccgctat cagggtatcc
      841 cgatgggcgg atatacacag ataatcgaaa aaatgctcgc aggctctgat gtaaagacag
      901 gcaccgatta ttttgagttt atcaaggaaa atcccgacat tgccgacaag actgtataca
      961 caggtcagat cgatgagttc ttcggatttg aatacggtcc gcttgagtac cgttctgtac
     1021 gctttgaaac agaggtactt gacgaggaaa accatcaggg caacgcggtt gtaaattaca
     1081 ccgacagaga agttccttat acaagaataa tcgagcataa gcactttgag ttcggcaagc
     1141 aggaaaagac ggttatcagc cgcgaatatt ccgcagagtg gaagcccggt gttgagccgt
     1201 actaccctgt aaacaatgat aagaacaatg cactgtatga gaagtacaag gctctcgcag
     1261 atacacgcaa ggacgttatc ttcggcggca gactcggaca gtacaagtac tatgatatgg
     1321 ataaggttat catagcggca cttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatatttg
     1501 tttatatatc ctactgatac agctcgcagc tgtcgggaga atagcagtca aatgccgaaa
     1561 gatagaccct gttatcattc tcataggcta tatagctgtc agaggctttg atcgatccgt
     1621 cagcgttata aatcactatc agatatccgg acatatcgtt atccgggcaa tcagcaacac
     1681 tgtttatcct ccacacggag gtatccggat ctttccacac agtaaacggt tttccgaccg
     1741 cctttgtaaa gccgtctatc gtgtcgtttt cctttatcgc attcaaggct gtatcaagat
     1801 ctataacaag actgtcgctg tccgtgtagg atatctcatt tccgataatc gtcatatcat
     1861 cggaaacatt gtcattcact tcaaaagatg cggcagtcag aggttttctt atccctgttt
     1921 taagctcatc ggcggcaata tcggcactgt agatacgcac actgtgcata ccgatatttc
     1981 ctgcatcatt agcatcgctc ctgcacgaaa gaaacagcat gccgtatatc tttccgccac
     2041 tgccaaggca aaggatatcg tcatattgat aaactgcgtt atcattgatc ggatattcat
     2101 agctctgtat atttgaacgt tccgatccgc cgtttttcag tattttaaga aagcctttgc
     2161 gataagccga cataaattca tctatctgct tatcaaaatc gggctgtgcc cttgcattat
     2221 cgctgtacag cgcaacaagt gcctctctgt tgccgctttc ggcggcttta agatattcct
     2281 cacggatatc gcagtaatcc catattaccg attgcataac gttgtcgccg gctttccagt
     2341 actcaacaac attgttatac tgcatatcgt ttattgcaag tcttaccacg cctaccgcaa
     2401 acatcgcaac tataaacgtt accaccgcta ccggtattcc acagaatata ccgcccgcga
     2461 ttatagatgc cttacgcggc tttatgcctg cggcaagaca ttttttcctg cgcaatactc
     2521 cggttatgag tagcggtaat ccgattgcaa gcggaacaat gatcagaagg aaagtgccga
     2581 ctaccagcaa aaatccactg accatcatca tagacataaa catcatatac tgcttcccac
     2641 ctttgtgctt tgactactga acattcacct caaacttgta tcctacgccc catataagcc
     2701 ttatgctcca cttggacgat aagccgtcaa gctttgactt aagcaggctg acagattctt
     2761 cgaccagtgc gtcactgccc tttgcatcgg gaccgaacac ctcataagca agctgctcct
     2821 tgctgaaaac nnnnnnnnnn gctatcaggc aacgcagtat ctcaagctcg ttaagtccta
     2881 tctgatactc attgctcccc actctgacgc aattattctc attgtcaatt acaagtgtat
     2941 catactcccc cgccttcttt ttatcatact tttcaaatac ctttgaaagc tgtgctatgt
     3001 caagcggcag tgacagagaa tgctcgctgc cgtcattttt cagtgtatat atatctgaca
     3061 taagcgatga aaggcggcac tcctgctctg catcaagata acagccgccg agtatgatta
     3121 tatcggggtg aagcatacag cattcacgaa caacgtgctg tgtatcttca cttatatcgc
     3181 atattgctct gacagtaaag ccctcttttg cagcatagtt cgatataaaa tccgatatat
     3241 gctcgttgtc gcaggctatn nnnnnnnnnc ttttcacatt ttaccccttg atttttatgc
     3301 gtaaagtgat tacaataata ttcagtggtt agcactcaaa cacaaagagt gctaacaggt
     3361 taaaaaatca aaagcaccgt cacagataaa aagtgacagc aaatataaca aggaggtcat
     3421 tcaaaatgac gatcaaaccg ttatcggaca gagtagttat caagatgctc gaagcagaag
     3481 aaacaacaaa gggtggcata attcttacaa gcgccgcaca ggaaaagcca caggttgcag
     3541 aagttgtagc agtaggtccg ggcaagacag ttgacggcaa gcttgttccc gttcagctta
     3601 aggtcggcga caaggttctt atgagcaagt actcgggaac agaggtcaag gttgacggcg
     3661 aagaatacac aatactgcgt gaggaagaca tcctcgcagt tgtagaataa ttgaaagtga
     3721 ggaacattca caatggctaa acagatcata tacggtgaag aagcccgcaa ggctttacag
     3781 gcaggtatcg accagctcgc aaacacagta aaaattacac tcggtcctaa gggcagaaac
     3841 gtagtgcttg acaagaagtt cggcgctccg ctgatcacaa acgacggtgt tactatcgcc
     3901 aaggaaattg agcttgaaga tccttttgag aacatgggcg cacagctcgt aaaggaagtt
     3961 tcgacaaaga caaacgatgc cgcaggtgac ggtacaacaa cagctacact gctcgctcag
     4021 gctcttatca gagagggtat gaagaacatc gccgcaggcg ctaaccccat ggacgttaag
     4081 cgcggtatcg caaaggcagt tgacacagca gtagctgaga taaagaagaa ctcaaaggct
     4141 attgagaaca gcgagggcat tgcccgtgta gctacagttt catctggtga cgaaacagta
     4201 ggtaagctga tcgcagaggc tatggaaaag gttacaacag atggcgttgt tactcttgaa
     4261 gaaggcaaga cagcagaaac atacagcgaa gtcgttgaag gtatgcagtt cgacagaggc
     4321 tacatttcac cttactttgc aacagataca gataagatga cagctaacct tgatgacgct
     4381 tatatcctta tcacagacaa gaagataagc aacattcagg acgttctgcc cttacttgaa
     4441 caggtcgtac aggcaggcaa gaagctcctt atcattgctg aggacatcga gggcgaggct
     4501 cttacaacac ttatcctcaa caagctgcgt ggtacattcg tatgcgttgg cgtaaaggct
     4561 cccggctttg gcgacagacg taaggaaatg ttacaggata tcgcaattct tacaggcggt
     4621 acagttatca catcagagct tggtcttgag cttaaggata caactatcga tcagctcggt
     4681 cgcgctaaga gcgtaacagt ttcgaaggaa aacacaacga ttgtaaacgg cgcaggttca
     4741 acagagcaga ttcaggctag aattgcacag atcagagccg ctatcgaaaa cacaacaagc
     4801 gaatttgaca aggaaaagct ccaggaaaga cttgcaaagc ttgccggcgg tgttgcggtt
     4861 atcaaggtcg gcgctgctac agaaatcgaa atgaaggaaa agaagctccg tattgaagac
     4921 gctcttgcag ccgctaaggc aggcgcagag
//
LOCUS       DAWAOG010000082         4941 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2224_length_4941_cov_2.203643, whole genome shotgun sequence.
ACCESSION   DAWAOG010000082 DAWAOG010000000
VERSION     DAWAOG010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2224_length_4941_cov_2.203643"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1003..1012
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1707 a          904 c          984 g         1336 t
ORIGIN      
        1 ttatatatct gatttaacaa aaaatatgaa atgtattgtt gaaatgggga aattaaaaaa
       61 atatcaacaa aaataatgtg tttaattctt gaaggtgcgc tctcacttga attagtagct
      121 tccgcaggtg cggaggcagg ggtcagcttt gaaactactg attacgaatc aacagatgca
      181 gtgtcagaag aaacagagtc tgaacaggca gaaatcctct atgaaataat atcgctgaga
      241 gacgagtaca gcaagagatt tatgctcagt gatggtaacg tacaaattgc acgttatgat
      301 tctgctgtcc attttcttga tgaaaacggt gagtggaagg atatagacaa ctctttacaa
      361 tcaagcgatg caattgacag tgaagatttc aacggatatg aaaataaagc caatgagatc
      421 aaggtcaagt tttcaaaatc ctcagacgca agcaaactgt tcaaatatca gtttgataaa
      481 agcagtatta catggggact tagcaacgct gataaaaaag cggctaaaat cagtaaaaga
      541 aatgctgtag ttcattccga tgatgataaa cttgcgttgg aaaatacaca atccggtgtt
      601 acatatactg atattatgcc aagcacggat ttgcagtata cggttattgg taatacgata
      661 aaagaaaata ttattatcaa agatagaaaa gacagttatg atttcacttt tgacattaaa
      721 accaaaaagt gtacattaca attggaagat gacggctcta tttctgtctt taataaggat
      781 aatgagcaag ttgcacttat tcccgttccg tttatgtatg acagtgaggg cgtgtactct
      841 cagaatgtca gctattctct gaacgagtat aaaaaaggta aatatcattt aacgataagt
      901 gctgatccag agtggataaa ttctgctgaa agacagtttc ccgttacgat agatcctatt
      961 atagaaaaga agtacagtaa cggaattaca ggcgctactg ttnnnnnnnn nnctgttaat
     1021 tctaagaatc cctcagtaaa tttcagcagc attcctgtat attatgttgg aaatgacaac
     1081 acattttata attcccgatc atatttcaaa attgatgttc ctgatatcgg aaagagcaac
     1141 attgttgtaa aagcagagtt caatctttat cagactgcgt gcaatccgag tgattttgca
     1201 gaaagaaaca tttatgctta taaactgaca caggattggg atgcaactca aataacatgg
     1261 aataataagc caaccaatca atcaacaata tcggattata tggctttaaa taagactaat
     1321 tccacagtta aaacgattga tataaccggt atggtaaggg aatggtacag cgatatttcc
     1381 aaaaattatg gttttgttct tatgggcgac agtgaacccg caacttacgg taaaggttcg
     1441 gctacatttg taaaatcact ggcaaacact aattttacta atgcacagct tccgtcattc
     1501 aaaattactt acagaaacaa taagggtata gaagactact ggacttatca ttctgtaagc
     1561 gccgataatt ccggaacggc gtatatcaat gatttcaccg gtaatttagt ttttgtaaat
     1621 acagatattg atgaaagcgg caacagatat ccgctaacgc tcagccatgt atataacgga
     1681 tataacgcaa aaaatcagta cagacagcct acaccgagca gcacagtatt atataacaag
     1741 atgaatttcg gattaggttg gcagctcagt gtcgaggaaa caatagttgc tgtattcgga
     1801 aattcaaaca ggtatgttta ctgtgacggt gacggtacgg agcatcattt gattaaaacc
     1861 gcaagcaccg gcgataattt tctggatgaa gacggacttg gtctgacatt aagcatagat
     1921 tcttcaagga attttaagct tagtgacaat agcggtaatt ataaaacctt tgatgtggca
     1981 ggttatctta cttcatgtgt tgattcaaac ggtcagaaaa ttaaatttac ttataataat
     2041 tcacagctgg tttctattac tgacggtgcg ggaagagcaa caaagctaac atatgacagc
     2101 aataactatt taacttcgat aaaggatccc gcaggacgag aaacgacgta tacctataac
     2161 aatgcgtgtt atcttaccaa aattacttat gcagatggca catattctgc atatgcttac
     2221 gattcagatg gtatgttact gagaacacac gacagcgaaa gtgcatacag aatacatttt
     2281 acatattcgt ctgataaggc aaaaagagta aaatcgtatc aggaatatgt taaaaccgga
     2341 actgagtata caccgggaca gaaggtgctt atttcataca atgtgagtga ctttgaaaca
     2401 acgtatactt cttccggtaa agataatacc ataaacacat ctgacgatat aaaaaatgtc
     2461 tacatatttg ataattatgg cagaacggtt tcttcatatt cgacaaacaa aaataagact
     2521 gagatatacg gagcacagtc tcattcatat gtatccgctt caaacaacgg aaaagcaaac
     2581 aaagttaaag atagtgcagg tgtaggagga acaaatccta actttcttaa atcaaatcag
     2641 tgtgaatcac tgtcaggctg gacgctcgga agtatgggtg caagcaatac ttccgtgacg
     2701 ggtggacttg acagctcgac aaaattttta ggtaatagtt ctctcagagt agcgtcaaat
     2761 tcagctgttt caaattctac cgtaggatat aagcagactg taacactcgg tgcaggcagt
     2821 tatactgcaa gcggctatat ccgtacttct tcggtaagcg gaacaggtgc aggcattgct
     2881 gttgccgcgc ctgacggaac ggttattgca cgttcaaaat gtgtaaccgg aacaacaaat
     2941 acgagtatag ataacggctg gcaaagaata agtatgtcat ttacagcgcc gacaagtgca
     3001 aattattcta tcatattcgg tataacgaat ggcacaggaa cggctttttt cgactgctta
     3061 cagcttgaat caggctcaaa ttgcagtaat gtaaatctgc ttgaaaatgc aggaatgcag
     3121 acgctgtcga attggaataa taatagctca aaatatattg ctaccggact tatcggaaaa
     3181 ggacttgcta tttccggcaa cccgagtgca aaaagatatt tatctcagac cgtggcaatt
     3241 aacacagctg caaatgatac atttgtatta tcaggttggg caaaagccaa ttctgtaagt
     3301 cagggtggca agcgtacttt tagactcaga gcagttataa aatacaaaga taatactcat
     3361 caggaggtta tagcaccgtt cctgagcaga tcatcggcat ggcagtatac cactgtttca
     3421 gttgtgcccg ggaaatcggg aatcattgaa aatattaccg tgtattgcga ctacgattat
     3481 aactgcaata ccgtgtgctt tgacaacatt tcgctgacca aggatattgc acagagctat
     3541 acctatgaca ataacggaaa tgttttatct gtagtggata acgccaagaa caaatccact
     3601 atgtcatatt ccaacaacga ccttgtaaaa caggtaaatc cgagcggata tgattataca
     3661 tacacttatg ataaaaagca caatctgctt actgctacca gtgaaaacga cgtgaagtat
     3721 gcttatacat atgacagcta tggaaatcct ttgacgctta ctgcatctga cagcggtaac
     3781 acgatgaata ttcgctcaac cgcggaatac agcagtaacg gaaattatct tgcatctctg
     3841 acagatagca gaggtcacaa aacaacttat gactattctg caaacacagg cttgttaaat
     3901 aaagtcaccg atccgctgaa caacagcgtt atatacagat acaatgccaa aaatcaacgg
     3961 ctgacgactg tatactatga taagaacaac agcaatactc ttgatacggg tgaatcttcg
     4021 gtagcttatg cttattcaaa aggtcgcttg tctgctataa ctcacaacga ctttaagtat
     4081 aactttgcat acgatgtttt cggaaatctg ctgagcacaa aggtaggcaa cattgcgctt
     4141 tctacgaata catacggtgc aaataacggt aatcttacta aaaccgttta tggcaacggc
     4201 tgtgctgtgg aatttgaata cgataatttg gatcgtataa cggcacagaa gatcgacggt
     4261 actgtcgctt acagatggta ttatgataat aacggaaata ttgacagaat gcttgatgtg
     4321 atcaacaata tttcttatgt ttatgaatac gaccttaccg gaagacttgt aagaagttac
     4381 tcaacgaaag gaacaagcaa gcttctttcg actgaaaatc agtatgatga tcagaaccgt
     4441 atcaaatcag caacatatat tcttcctaat aatgccaagc agcgttattc ctattcttat
     4501 tcaagtgata accttataac cggcgtttca ttgccggcgt caagcggtct ttcatatagt
     4561 tatgatgatt tgaacagacg tacactgaga agaatagcag gtcagaacgt tacaacacaa
     4621 gctgaaacct atagctacta ttcgcccggt cagtatgtcg caacaaatct cgttaataag
     4681 ataacttatg cggataacag tacgctgagc tatatccata acgctaacaa ttgcatcact
     4741 tcagtaaccg atgaaaacgg caaacgaatt acatacaaat atgacggcat gggtcagctt
     4801 ataagggaaa ataaccctta taccaacctt acaacagtat attcttacga caaaggcgga
     4861 aatattacct cggcaaaaga atatgcgtat acaacagccg cggagcttgg tacagtaaag
     4921 aagacgaata cttatacata c
//
LOCUS       DAWAOG010000083         4906 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2247_length_4906_cov_2.226139, whole genome shotgun sequence.
ACCESSION   DAWAOG010000083 DAWAOG010000000
VERSION     DAWAOG010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2247_length_4906_cov_2.226139"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1384 a          937 c         1164 g         1421 t
ORIGIN      
        1 gtaataacaa gtacgcgtat agataagaat gtttcgaccc gggaatattt caagaaggct
       61 ataagcggtg aaatcaacat ttcaaaggct atattcagcg aaccggacgg gaaaatggtg
      121 aatgtcatcg cagtgccgat atacggcgat tatcagcgta ttgtcggtgt tgccgcagga
      181 atatgcgact tgaatgtaaa tgcaaaacag ctggatttat cgttttcggg taaagaatat
      241 gacagcaacg gttatgtgct aaattcggac ggcgaagtgg tgatgtcggg aagcaatgcg
      301 atcggtgaca atgttccgat gggggatctg ttcttctcct ctgatttttt agccggtgta
      361 aatgaagctg acagaagcac tgttaaggat aactttgcca aggtaggcgg tagaggaata
      421 atcaagagct gctgcaacgg tgataattat atagcttatt acagtggttt taccgagctt
      481 gatgatctgc actatctgct tattttcaga gaagatgtcg ttgccactca acagaattat
      541 tatgcatttg tcaatatatt gatgtatggg cttttcctgc tgatacttgt gttggtgatc
      601 attgcatatg ttgttatagt cagagtatcg ttccgcagat tgaaaaaggc caacgcagag
      661 gtcagccgta tcgcttatac tgacagattg acagggtaca gtacatggga taaattcgtt
      721 atcgatgtag gcggcttgtt gaaacacgaa tatcgcagat atgcacttgt aagctttgat
      781 atcgataagt tcaaggctat aaatgatatg tacggtcacg aagagggcaa cagagtgctc
      841 aaactgatag cagatacggt aaaccgcaac ttgcaggacg gtgagacgtt ctcaagaata
      901 aactcggata atttctacat attcatgttt tatcacagtg ataatgacat atcgagaaga
      961 gtaacttcgt tgatacaggc tattgagtac gaagtaacgg agtttgttcc tgtgctttct
     1021 ttcggaattt acagaataac ggataaaagt gtatcgataa gacgaatggg cgaccttgcc
     1081 gatatagcaa aacgtactgt gaagtatggt gatgacagcg catatgcatt ctatagcgaa
     1141 agtatgcttg aggaaatgcg tgaggagaaa cgcattgaaa atgagatgca gtcggcgctt
     1201 gatatgcatg agttctgcgt attctatcag ccaaaggtat cgcttgacgg taaggtaaat
     1261 cttgccggtg cggaagcgct tgtaagatgg ataaaggacg gaacggtcat ttctcccggc
     1321 aagtttatcc cgctgtttga aaaaaacaga tttatagtaa agctcgactt ctacataatg
     1381 gatcaggttt gtcagaaaat aaagaactgg cagaagtatt acaaggatct gctgatatct
     1441 gtaaatatgt caagggttca cctcaaagat ccgcagtttg ttaaaaagct gaatgatatt
     1501 tgtataactc atggcgtttc aacttcaagc attgagatcg agatcacgga gagtgctgcc
     1561 tatgaatcgc ttgatgttct gactacagta ttcaaacagc tcaaggaata tggcttccat
     1621 atttcgatcg acgatttcgg atcgggctat tcctcactca atatgcttaa ggatctgcct
     1681 gcagatgtgc tgaagataga cagggcgttc cttgcggaat caaacagcaa caagagagcc
     1741 aacgatatac tcggttatgt tatcagaatg gccggctcac ttggtatgca tactatatgc
     1801 gagggtatcg aaaccaacga acaggcaaag ctgcttggcg gtcttggctg tgagatggca
     1861 cagggcttct actttgcaaa gcctatgccg agcgattctt ttgaagagat actaaagggt
     1921 aacagcaaga ctataaatat tccgagtaac gatcctgagg aggataaatg agcatttcta
     1981 atgagtccct gccgataata gcggggatta ttaccaatac ggcaaggtca atgactatgg
     2041 taatgcagta tatctatact gtgtctgaca gcgattttta caacataaat atcaaggacg
     2101 ttttcaggat cgcgcttatg gacgttacag aaacgtcaag gcttgaaaat ctcgggataa
     2161 ggataaagac acctgaaaat gatgctatgt ttgaaacggc tgagttcggc agggtgcagc
     2221 atcttatcat gtacagtctt gcggtgcgtt tgccgtttat agcacgtcag acggaagatt
     2281 ttccgctgtc ggataaacag cttaagcagg tgtacgagat tatgctaaag aacggtgcgg
     2341 ataatttcgg tgatatcatc tatgagagct atgagggcaa ttttaaagtc agaaagcaga
     2401 aaacgccgtt gccgtcgtat tcatcggact ggttcaggcg ttatgtttac acatatatgc
     2461 caaaattcgg tgagataaac aaccgtaacc tttatttcct cggttgtgtt gaggctatgt
     2521 ttccgctgta ttattcggcg atgatatctc agcttaaaaa ggtaatgttc ctgcttgata
     2581 agtaaaagat gaaaaatccc acaacgttta agacggttgt gggattttgt tgatacgaaa
     2641 tcaagtaacg gattatttta tcggaacagc tgtaacgatg caaaggagga taacatgaga
     2701 gagcttgaaa caaagcgact gttgctcaga aagctgagga aaaatgacgc agagagtatt
     2761 ttcggaaatt gggcgagtga cagcgaggtc gcaaaatatg ttacatggaa tgcgcataag
     2821 gatataaacg aaaccaaggc aattcttgat atgtggctgg cggaatatga caacgacaac
     2881 tgttaccgtt acggcattga gaataaggca gacggtgagc ttataggaat gatagatgta
     2941 gttggttatc atcacggaaa tcccgtgata ggatattgct cctgccgcag atattggggc
     3001 aatgggttta tgaccgaggt gctgggtgct gttatgaaag agcttgcgga ggacggattt
     3061 gacacatttg tcattgaggc ggcggaggac aatgttgcaa gcaacagagt tattgtgaag
     3121 aacggatttc agcttgtggg gaagagagag gacaggctgt cggaaataaa gccgcaggtc
     3181 gttacgataa attcgtatag gtattatgtt gaaaaataga tacaacaaca gccgcccgaa
     3241 tgggtggctg ttgtttatcg gtgcggtttt atgatataat atctatatta tactcgattt
     3301 tcatgcgttt ttcatttaat tttatcagac acccaccgcg gctattacac cgcggcagtg
     3361 ccaaattcaa ttatttattt tgccgccggc atcaacgcta tataatcatt cgtgatactg
     3421 ttttcgtctg ccttatatgt tctcttctca gcataaacag gtattatact gccgtccttt
     3481 gtctttgcgt aagtaagtat catatcattt tcagccgtgt actctgtttt cagaagttca
     3541 ttgtgtgtcg gacaatattc atcatcctcg ccgtcctggt gcatacccgg ctgaacggcg
     3601 taaacaccgt cgtttgccgc cattttcttc ttggcatcat catacggcag gatatttacc
     3661 gtacctgttt tcttgacata cggtgctatt ccttcaaccg ttatataagt atcacagccg
     3721 ttataatcga tagtgacggt cttgcagaac atatcgttat aatcaagtat ctgctcttct
     3781 gtcgtagcgg gctttttcgt tttcgccgta tatcttatcg tgggatagaa tgctttatct
     3841 tcatttatct catacgtttc aaaagagctt tcctcatatc cttcgccgaa aaccgacgag
     3901 tgatttttga tatactccgc aaactcaccc tttatctttc caagctgttc atcggttaca
     3961 taaagcgaat ccgtccgatt tttacggtgt tcaagcgcca atgcatacag cgtttccgac
     4021 ggatatattc cggctatatc cgtcacggaa ttatcatctg tgctgaacga ctgtatacgc
     4081 ataacaagtt tgcctttgtc gtctacccat atgcagatat ccggagatga aagccctccg
     4141 ccgtaagcga ccttatacag tttatttctt atgtcatacc atacgctctc accgtacaga
     4201 taatcaacat cgggataatc cttgcatgta aagaacgctt tatcgacctg ctttattccg
     4261 tcctcactgt acagctcaat cttaccgttt tcgttaaagc tgtatgtatc aagctgagtt
     4321 accgatgcaa gaccgtctat cgtgtatctg ttgctcaagt cccccggcga accaagaccg
     4381 acagaaaatt tatcatgttg caccttgatg aaatctcttg cgaacattgt tttttcgtta
     4441 attccgtttg aggtggaata atcgctgtgt gacgatgtgt tgctgtttcc gctgaaaaat
     4501 gcgctgagat attctgaacc tccgagcgct atcattgtgg cggtcactac tgcaacaagg
     4561 cagaagcagg cggctgaaac gccgagaaaa gcggtattca ggtaccactt tttttggcgc
     4621 ggcgtttcgt caagtgcatc ggaaacgtac ttctcgttta tgtcaccgaa tgcagtaaaa
     4681 agagggaatg ctttatgctc gttcataatt tttctccttt ctgaatgtat acttctttct
     4741 ccgaaaggca ctttctgagt ttagtccgtg tgcggttcag aatagatgtg atgttggttt
     4801 cggtaagtgc aagcctttga gagatatcgg ctatgctgta gcaggcaaaa taacgtaaga
     4861 cgaaaatttt gcgtgtacgc tcgtcaagct gagctaaaaa tgcgtt
//
LOCUS       DAWAOG010000084         4888 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2255_length_4888_cov_2.150010, whole genome shotgun sequence.
ACCESSION   DAWAOG010000084 DAWAOG010000000
VERSION     DAWAOG010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2255_length_4888_cov_2.150010"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1194 a         1102 c         1047 g         1545 t
ORIGIN      
        1 cagcggctgc agaactggca cgcctgcaaa acctccgtac cctccgcagt caaacagata
       61 aggcgatggc gctttacata agaactctga aaaaacagcc gaccgtattg agcgagtgga
      121 acgacacaat ttggactgtg atggtagaga aagcaatcgt ccacaggaac agcgagataa
      181 cctttgtatt ctacaatggc actaaggtta gagtggagga ataaaattac agagccatag
      241 gactgctcaa attttaatga gtaagtcttg tggctttttg ttatttttag gaataattaa
      301 aaagtgcata cccccttgcc gaaaatgcac acccttacga cctttcgtta aatagtataa
      361 aggcgaaaaa aagaaaaggc tccgctgtga ccgcttttta gtcatcgcag agccttcttt
      421 tgttaaattg tgttgacaaa tcccattaaa taaagacttt cggataaaga aaacggacac
      481 cgctcaagat acgcattgta tcaaaattgg tgtccggtta tggtgcaggt gacgggactt
      541 gaacctacac gactatgcgt ctgctagcac ctgaagctag cgctgtctgc ctgcggaaca
      601 agtttcgcta ccgtgaaatc tttgatttca cgcaattccg ccattgtgga tttacggata
      661 taaaaaaaca agccgtattt ctacgacttg tcactggtgc aggtgacggg acttgaaccc
      721 acacgacctt gcgatcacta gcacctgaag ctagcgcgtc tgccaattcc gccacacctg
      781 cgtattcaac gttaatatta tatcataatg cggctgcaaa gtcaagtgtt ttttgatttt
      841 ttttgaaatg tgtttgactt tggtatttta aaagggcatc gcttttaatg cgatgccctc
      901 ttgaagtatt gattttgtct gatattcctt atttagcagc agtttttccg ctgatacttc
      961 cgtaattgcc gtaaagcgga gtactaccgc tcatgtgata agcacgaacc ctgaacttat
     1021 aggttgtatt ctttgcaagt ccggcttttc tgaaagtagt tgtgctgttg tttgttatct
     1081 tagcaactct tacccactta ttgccctgat acatctctac aatgtagccc tgagcagagg
     1141 tgttctttgt ccaattcact ctcagtgcgt ccttagcctt accgccgatt ttaacgcctt
     1201 tcattattga aggattagtt cttgcggtaa cagtagcact gtagttaccg taaagtgcag
     1261 tgcttccgct catcttataa gctctgactc tgaacttata aacgcttgaa gccttaagac
     1321 ctgcttttct gaaagtagtc gtgctgttgt ttgtgatttt cgcaactctt acccacttgt
     1381 tgccctgata catctcaaca atatatccat cggcagaagc attctttgtc cagtttacgc
     1441 ggagtgcgtc agccgctctg ccaccgagct taacaccggt caccttggca agcgcgagct
     1501 tagctgtgta agtgtccttg taactgttgc cctgatacat ctcaacgata tatccgtcag
     1561 cacttgcatt cttagtccag ttgacacgga gcgcgtcagc cgctctgccg ccaagctttg
     1621 caccggttac gaccgatggg ttggttctta tcgcaacggt agagctgtaa gtgctgtaaa
     1681 gtgcagtgct tccgttcatc ttgtaggctt tgattctgaa tttgtaaaca gtcgaagcct
     1741 taaggctgtt tatgcttaga cttgttgttg aattgctcgt tatcttcttg agtcttaccc
     1801 attttgcgcc gtcgtaacgt tcgatgatat atccgtcggc acttgtgttt ttgttccact
     1861 taagtgttat gcttgtaaca gcggaggatt tctttgaaaa tccggttact gcggacgggg
     1921 cagaggagga ggaggaggga gatggctttc ttcccttttt gccgtacttt atcactgcat
     1981 caataaagct cttgtcattc catctgagtg ttgaacggtt gagatgtccg tggcattcgc
     2041 ccttgtcgtt gccgttataa acgttattgt cccacagcat acaaggtacg ccgtattctg
     2101 ccgatttgcc catataatac tgcgcccatt ttacacgctc ggcagagtta ccattcttgt
     2161 ttgaagcact ggtttcaccg attattgcag ggataccttt atcgataaaa tgcgatttta
     2221 ttgtgttgta cagataatct acttcatttt tcatatcatc cttaaattca gctgtgccgt
     2281 ttgcgttaag agcaaaatta taaggtgtat atgcgtgtac cgatacaata agacgatcgg
     2341 gcgtagaatc attcggtaat ttgaatgttg atattgtaca gccgtctata gatgcaccgt
     2401 atcccggaac cattatacat ctgtcgttat ttttgccacc tgccgcacgg atagtgtcga
     2461 ccgcagtctg attaagtgtg tttataacat tgatggaatc aactaccgca ctgctggggg
     2521 aatttacagg gaaccaccat tcatcgcccg tgccgacaag tcttggctcg ttgagcgttt
     2581 caaataccag tcgctgatca tagtccttga aatatgccgc aacctgtgac catatcgatt
     2641 ttacaaactt cacagactgc gtcttgtatg ttgatgaagg gtagtagtac tgtgttgaag
     2701 tgtcgtgatg aatgttcagg ataacataca tatcattgtc aatacagtag tctacaactt
     2761 cctttactct tttgagccac gcagagtcga ttgtgaagtt gttgccgctt gtgtgatttc
     2821 cccacgatac aggtattctt actgtgttga atccctgagc tttaacggcg tctatcattg
     2881 cttttgttgt ttttgggtta ccccatgatg tttcgctgtt tacgctgttt ccaccccctg
     2941 tagcgtcaag cgaatttcct aagttccagc ctgtgccgat ttcagcggtg atctgctttg
     3001 atgttttgcc tgttatcgtt gcagcagagg cgctcggcgt tgcacacacc ataagtattg
     3061 cagctgcaag tactgcaata aatgccgcga cagccgaaaa ggttttcttc gttattgtca
     3121 ttttttctcc ttgactcaga attataacat tctgataata atattcttta tttcagagag
     3181 cggagcctgt tttgatactg caccctcctc aaaagctaca cacggcatac cgtatacaac
     3241 gcaggactct ttgtcctgac ctatagtata tgctccgttt tttttcattt caccaagccc
     3301 ggatgcaccg tcgcgcccca tgcccgtgag tataactcct atcgcatctt ttcctgcggc
     3361 ttttgcgacc gagctgaata atacatctac agaagggcag tgccctgata ctttttctcc
     3421 gggctttgag gttacataat agccaagagt atctttatga acttcaagct gttcagagcc
     3481 gtgacccagt attataagcc cttgtctgag cctgtcgccg tcttcggctt cctttgcatt
     3541 cattttgcat attctgtcga gtctgtcagc atacattttc gtaaatccga ccggcatatg
     3601 ctgaactata actaccggag gagtatctgc aggcagttct ttgactacct ctataattgc
     3661 atctgtaccg ccggtacttg ctccgatagc tataaggtcg ggtctgcctg cgtgacagac
     3721 attgtgtatt gccgtatcgg cgcttttatg aactgtcttt tttgaaaaaa gtatttcagg
     3781 gtcgcttccg tccggtgact gcattatttt tccaaaatgc ggatttgctt ctgctttatg
     3841 cggatattcg gttgcaacag ctttgtctat cagagagtga agctcttttt caaaaaccgc
     3901 aagccctgtt gctccgccgc ttgaattttt caacgcgccg aaaagaccga aatgctttgg
     3961 ttctttcagt attctgtcgt tgttggttat aagtatagaa ggtatcccgt tcagtgcaga
     4021 cggcaaatga aaatatgcat cggctataag gttgttgatt attgcacagt cgcaatgcgc
     4081 gggatttgcg gataacgcta attttctgaa atcgacaatt tctatcgaac agtcctttat
     4141 actgcgttca agaattgact tggttatttg gcttatagcc gtacttgttc ctgttataag
     4201 tatgtgaaga ggcaatacct cacctccgat tgcggcgttt tgccgcatat ttgtcaagcc
     4261 gtattattgc aacggcttac ggtatattgc aggtttgata tattgatact gggtcgtatc
     4321 tctgggtatg ctttccgcat gaccgataaa cagatatccg cccggtctta acacatcaaa
     4381 gaaacgattt acaagcgcaa gctttgtttc catctcaaaa tatatcataa cattgcgaca
     4441 aaatatcaga tcaaacggag cttttttgta tggaatcggc tccatcagat taaatgtacg
     4501 aaatataact tcccggcgca attccggttt gatcttgcat ttttcgctgt ccaatctgtc
     4561 aaaatatttg tcgatccagc catcgggaag atctgacaat ccgcttgcaa gatatacgcc
     4621 gttttgtgct cccgcaagag tgcgaagaga gatatccgtt gcaagtatcc ttgtatccca
     4681 tgcggttttc tccttgccga gaaattcact tatgtgcata gcagtcgtat atgcttcttc
     4741 tccgctggaa cagccggcac tccatattct catcgttttt gtcttagcat ttacctgctt
     4801 taaaaacggt aaaactgtat cggtaagata tttaaagtgc tcaggctctc gcataaaata
     4861 tgtatgattg gtagtgagct ttataagc
//
LOCUS       DAWAOG010000085         4862 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2269_length_4862_cov_2.211774, whole genome shotgun sequence.
ACCESSION   DAWAOG010000085 DAWAOG010000000
VERSION     DAWAOG010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2269_length_4862_cov_2.211774"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2703..2720
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1463 a          984 c         1223 g         1174 t
ORIGIN      
        1 gtacagattg atgtatggga taaggctgat acacctgcta ttgtagccga tatgtcggca
       61 cggataagcg ctgtaatggt atcgaaaggc tttcgccgta tattcggaca gatgatgccg
      121 gacggcgatc tacagcgtaa atgtatgcgg ttttccgcaa aaatagatga actaaatcac
      181 agggtttata gcccttaagc agaaaggaaa attattatgg aacttttatc aaaaggcaca
      241 aaattacagt atgccgacac aaagacaggc acatttaaga cgctttacgg cttacagtca
      301 actcccgata tgggcggcga tcccgaaaag gtcgatgtaa cgaacctggc agacggctca
      361 aagcgctaca tacccggtgt caaggactac ggcgatctgg acttcacgtt cttttataac
      421 gatgaagatg aaaatcctgc cgtgtccgaa gcggatgtag cggcggcgta ttccacgctg
      481 agagcgttac agaaagcaaa ttctactgca tggttcaagc tcctttaccc cgacggtaca
      541 ggctttctgt ggagctcaaa ggtatcggta aagcgttctg cggcagaggt caatgcggcg
      601 ctgaaattca cgctcagaag cacaccgctt acagagcttg aggacgtaac tgcggcataa
      661 cttgacattt atagcccttc gtggtattat agatacaaat atagtatcac ggagggaata
      721 taatgatttt agctatcgca tgctttatcc ccggaataat acttttgttt gcttctgtat
      781 atgctttttt taccaatcaa ggggcagttg gagcagtaat gcttgttatg gctttgatat
      841 ttctgtttta cggtttatta agaatgaaaa aagttaaaaa agcaaagaaa agaggtacat
      901 caatccgtgc tcttataaaa tgctacggta tagcgcttta cggattaccg caaggagaga
      961 gcgaatgttt attatctgtg tttaaagata aatcggtttt cactgtcaat aaaaacgatt
     1021 ctattcttaa tgcggataaa ataacgtctg cacttttgaa atcaaactct gagctcaaag
     1081 gagcgtctgt ggggtctact gttgcgggcg cgctgttgtt cggtattccg ggtgcaatta
     1141 tcgcatcacg tcctaagaat ataactgaat atgttattat aataaattac ataagtggca
     1201 gtgagttaaa gacgattgcc gttgctgtaa cagaagatag aaagtatgag gaagataaaa
     1261 tcgttaatta tgtgaataag cacatggcga gcggaagcga cacagtgcta taattgtgtt
     1321 aaaaaattaa atttataaag cacatctgaa agggtgtgct tttattatgc ttaaaattaa
     1381 acggaggata ttaaaatgga agaaaacaga ttaccctatg aaacactgaa aatcggcgat
     1441 accgagtaca agctcaaaat ctcggcttca tcggcaatcg agatcgagaa gaaaacaggc
     1501 aagtcgcttg ttgcgggtat ggcggatttt gacaagctcg aaacagtcac gctgtatctg
     1561 tggggcgctt tgaatcgctt ccaggcgaat attgacatca gaaaggcgca ggagatctat
     1621 gacgattaca tagacgcagg cggcgacctt tcggatatgg cggagatact cttcaagacg
     1681 cttacggtgt cgggtttttt caagcgtcag caggcagaaa aactgctggc gctcgcagaa
     1741 aaggcaggga gtggagcagt gcaggagaac tgataaccaa cctctaccgc ccggcgctga
     1801 cagcaggaat aacacataaa gatttctggg acttatcggt acgggaaata acgcagacga
     1861 tacaggcaaa aaacgaatac gacaaggcac gcgccgagct taacgaacgc ttgatgtgtg
     1921 cctttgctta tagcatcggt cagcttgttg ccataggtgt caacgcacca aggcaatatc
     1981 cgcacagcat cgaaaaggca tttcctaagc tggacggacg tgataattca gagggaatac
     2041 ccgtgtcgga ctgggagtta tcaaagcaga atatggctga atatgcggcg gctacgaaag
     2101 gcaggtacag taagtgacag tagaagaact gaacgttata gtaagtgcaa ataaggatga
     2161 ttttgatcgt aagataaggc agataaatac gcagcttaat caaatgaaaa agcaatcgga
     2221 gaaaacttct gacagcgtta tggacacgtt cagaaaactc gcttcgggac tatcggcgct
     2281 tggcttcggt gcaatgataa agaatgctat cagccttgcg ggtgaccttg agcagaatat
     2341 cggcggttcg gagtcggtat tcaaaaatta tgccgggaca attcagaaaa ccgcagaaac
     2401 tgccgcttct tcgctcggac tttcacagag caagtatctt gcgactgcca caaaaatggg
     2461 tgcgctcttt cagggttcgg gtttttcggt tgcacagtct gccgatatgg taacgcagtc
     2521 tatgcaacga gcatctgatg tggcaagtat tatgggtata tccgttgaca gcgcaatgga
     2581 agcggtcgca ggtatggcaa agggcaactt cacaatgatg gataatctcg gtgttgccat
     2641 aaacgatacg aacctgcaga tatatgctca ggaaaaagga cttgggaagc ttgaaaccac
     2701 acnnnnnnnn nnnnnnnnnn ggtcaacgcc gctatgcaga tgttccttga taagtcggaa
     2761 tatgcggcag gaaactacgc aaaggaaaac gatacctatg tctgacagta caggcggtca
     2821 gtcggcagat atacacgtca cggtcgatat agacggagat acggtcggtg aaaccgtagc
     2881 gcgctataat gctgtcagaa accgcagact taacggaagg agttaatatg cagacactta
     2941 taaaattcgg cagcttcacg ccgatttcgc cacgctcata cgctgttcaa cgttccgatc
     3001 ttgacagtga ggacagcggt agaagcgaaa caggcgtgat gttccgcaac cgtatcagag
     3061 cgggcgtgta caagatacag gtaacttgga gggtgaacag gtcgcagctt tccgctatag
     3121 caaatgcgat ttctcccgat tcgttttctg taacattttt cgacccgacc acagcgagta
     3181 caaagacctg cacaatgtat ctgatagcgc tgtcagaaac gccacaatgt ttctgccgga
     3241 aaacccgctt ttgcagtcgg tatccgatcg caaggcggcg tttgaagctt gtgccgatgc
     3301 cataaatata gcactgcgcc gtgtggagtt ttctttcaac ggaaacccgg caatagaatg
     3361 tttcgatacg ttatgcggta acggaggaaa gatagatgta aaccgcacaa tagcattttt
     3421 cccgacctcg cttgtatgga aatacagagg ggcacataag gtcagctgtg cttttgcgga
     3481 gctaacagat gaagctactg caagtgtatc tgaggtggca acttttgcag cagctgtagc
     3541 aaataaatca ccgctcagta caaaaaacaa gactgataag cgtatagatg atgtggcaaa
     3601 gcgtgctaac ggaataggtg atcagataat acttaccgaa aattcggtaa aatacctgga
     3661 ttacaattat gacgtggtcg gatattacgc aggtgataca gcaacatatg gaattagtgg
     3721 caacgatatt attgtacagg gatacgtcct aagccctaac ggaaaaccaa ttgccgactc
     3781 tgctaaatac tcagtgtata cgtttacaaa tacaagtcta tttcaaacag ttaccatata
     3841 tccgatgacg ctggtaaaag cagtagagca aaaagactca gacggcaacc tctatacgtt
     3901 gtattacgtg catatggagg gtcatgggat aaaaaccgat gggacgtcat gggaagtaag
     3961 cggaaattcg ttaactacgc gaaatacaga ttggggaggt gcgtatcggt actctgcaat
     4021 aaatattcct gatgatagtt acccgtatgg aagtgtcttg atatcgcctg ccgcgtatat
     4081 tgttaatgaa aacggcacgt attcagagtt tacaaatccg tctgtcgggc gtgtcccatt
     4141 tgcttcgatg gcggagtata atgcggcagt tgggctgact agatcaccgc tacagcggca
     4201 acttgtatca cagacagtga aaaaagacga aaattatgat actcctgctg gtttaccgca
     4261 aataggcaca tctgatacgc tgtatacaac gcttaacccg cctgctatat atcgctatga
     4321 cagtagcacc ggcaaatata tctgtgtggg ttctgacttt tcagccattg ccgaaatcaa
     4381 atcggataac tatgctactg atgaatataa tgtgctgaaa ttacctgtcc gacagctgac
     4441 aaaatctccg tccgcctggg cggcagataa tacagtgtat ccaagcggta tgctgctggt
     4501 gtcgcaggat ctgacgacac aggcaggtag atatataagt gatatcaggg ttgcaaacgg
     4561 catagataaa tggaaaatgc taaaaacaat atcgcctgat atgtgcgact atctgctgtc
     4621 aactgatata gcagactggg cgaaagctga tgaaaagccg tcatataccg ccgaggaggt
     4681 cggtgcggcg gcgaaaaagc acacgcacac taaatcggat ataacagatt ttccaacgct
     4741 gggaacggcg gcgggaaaga atattgatta tttcgcggtg gcggagcata cgcatacggc
     4801 ggctgatgtc ggtgcggcga ctgcggcgga tatcacggcg gctgtaaatg cagtcagcat
     4861 ag
//
LOCUS       DAWAOG010000086         4850 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2275_length_4850_cov_2.377059, whole genome shotgun sequence.
ACCESSION   DAWAOG010000086 DAWAOG010000000
VERSION     DAWAOG010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2275_length_4850_cov_2.377059"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    576..597
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1095 a         1261 c         1058 g         1414 t
ORIGIN      
        1 tcggtaatgt cgctcatgct ctgacccttg aggtcaagaa agaacttcat atcatcattc
       61 gatgtctgct ttagcataaa ggtcttgagc ggctttcccg ccgcgtccat agcctgttcc
      121 atcgttattt cacccgccgc atacggctga tatgccgttt cgtttatctc ggagaaaaca
      181 ggccacatta tgaatatcgt cagaaacagt gacagtcccg taagcaccat attaggcggc
      241 gtggactgtg tctgcattgc cgttcttaaa aagctgaagc agattattat cctcgcaaac
      301 gatgtcatca tcacgagaag cgacggcgca agcgacagca ccgttacaag cagtatgact
      361 tcaagcgact gcgaaccgtc aacaccgata agatccttca ttaccgaaga gccggcagac
      421 tccgtaccgc ctgacgaagc ggcagatgcg ctgcccgatg tcgcggctga tgccgccgca
      481 ttacccgcgg cgttattgtc tgtcgcaaat gccgtaacac tgcaaagcac cccgataagc
      541 gcaacacata ccgttaaaga cacgataaag cggatnnnnn nnnnnnnnnn nnnnnnncgt
      601 cggcactttc accgccgtta cttttctttc cgcgcatatt ttttatattc ttggcaagtg
      661 catcgctgaa cgccgcaaaa aaaccgtcct gctgtgcctc gccctcggga tagagctgtg
      721 acagatactc ctcctcggtg agtgaaagat cctctatctt gtctatcctg ccggcggtaa
      781 ctccgagcac catacacttt cccgccacgc ttacgacaac tatcgatttt tcgccgccga
      841 gcgttgcgcg gtcaaggatc ttgagccgtt tcccgcctgt tgtaagtatc cctttgttta
      901 ccttcctcag tacccagaag aacaaaaata tcagcgcaat tacgccgaca agcgcacata
      961 caagctgaac aaccggaggc atcagtcaag tattttctta acagtctgga gtattctctc
     1021 agccttgaac ggctttacga taaagtccag agcgccgagc ttgatagctt caactaccat
     1081 agcttcctga cccatagcac tgcacattac gaccttagcg cccgcgtcca tagccttgat
     1141 ctccttgagc gcctcgatac cggtcttgtt gggcattgtt atgtccataa taacaaggtc
     1201 gggcttctcg gcggcatatg tagatacggc gatttcgccg tcagccgctt caagaatgtt
     1261 tgtgtagccg tttgttgtca gtgtgttgcg gataagcata cgcatgaatg ccgcatcgtc
     1321 taccagtaat atcttcttat ccataagtgt aaggtttcct ttcgtatccc tgttttatta
     1381 tatgttattc gcctttaccg aggctgtcca taaatttcga ttttactatt tccgttattc
     1441 ttaccgcaaa gttatcgtct ataacaacaa cgtcgcccct tgctataagg ttgccgttta
     1501 ccataacgtc aacgggtgcg cctgcctgcc gctcaagctc aatgatagtg ccctgagaaa
     1561 actcaagtat atccttgatc tttttcctcg ttgaacctat ctcaacggat atctgaagcg
     1621 gtacgtccat aaggagctgt aaattttcct tctgctcagt accgagcgga gcttcaaaat
     1681 tctcaaactg tgcaagctgt gcattgcggt agttaaccgc aggctgcggc tgcatcatag
     1741 gctgtaccgg ctgagcatac atagcgggat taggctgacc gggataaccg taataacctt
     1801 gttgcgcata catatccggc atcggttgcg gagtcggcat aggtgctgtc ggctgtaccg
     1861 gctgttgagc aggcattgcc tgcggtatcg gtgacggtgc aggagcgacc tgctcgaccg
     1921 gagaaggcgc cggtgtcggc gcgggagtcg tttgcggctc gggtacggga gcctctgccg
     1981 aagctgcacc tccgagaacc ctgtctgcaa gcgttgccgc aagatttata tcaagtaccg
     2041 atataaattc cgattttata acgccgtcaa tggtaaggtc aaaattgata gcggcaacca
     2101 tatcggtctg ctcatagttc tgaaggtctg ctatgtttat ctcgctgagg atatacggtg
     2161 tcggcgttga aatatcaatg ctcattccga gaaagtcaga cagagccgtt gatgatgcgc
     2221 ccatcatctg gttcataacc tcgcttaccg cacatattaa gctcgtcaaa ctcaaagtcc
     2281 ggtgatataa caggcggctt acccatcagc tgattaagta tcagctgcac atcgtcctcc
     2341 tttaatatca tcagattaga gcccgaaaga cccttgatat attcgatttt tacacatata
     2401 gccggctcta agcggtcgta attcagccgt gaagcctcca caacgcttac cttcggcgta
     2461 gtgatccaca cttttgcgtt gagcagctcg gatacggcag ttgccgcact tcccatactg
     2521 atattcatta tctcgcctat agcgtcaagc tgcatctccg acagagtggt cagttttccc
     2581 atttgcctta cctcaattct ttagtatatt atcgatcctg aacgcttttt tcccgttcag
     2641 cgtaccgagt tttccatcaa accaacaggt gcttcccacc ttcagctcca ggttttccgt
     2701 tatcggttta ttcagcggaa ttatgtcgtt tatctgtaaa ttcaatacat cacggagcgt
     2761 cagcattgtg tcgtcaagca ccgcagtaac ggtaagctcc gactgagaaa gggtagacat
     2821 aaggttttcc ttgcgctccg tttcctttgt cggactgccc gctctttttc ccgagctgaa
     2881 cttggccgaa aatttcttca ttatctgatc catagtaata gcggacatac agaacgatac
     2941 tacggatttt gaatcgttta tggttacttc catagcaacg attatcattg tttcgtcagc
     3001 gtctgcggcg gagataacac gcgagtttgt ttctattgat tccagcttcg gttttgtttc
     3061 gacatagccg tcccacgcct ctgtcataag cgaggtaaag cgctcgacaa tgcctcgcat
     3121 tacgtttatc tctatttctg taaagtcacg gtcggcatct tcgtatgtgc ccttgccgcc
     3181 gagcattcgg tctatcatag taaaggtgag cgagtttgaa agctgcaata ccgcattgct
     3241 ttcctcaatg tcatcatctt ctatgccgag gtcaactatg cccattatcg tataatcggg
     3301 cagggcgttg ctgaactcaa aatatttctg ctcttctatg cttgcaagcg aaaccttaca
     3361 atacaatctc agcagtccgg ttatgtacga agaaagatgt ctggagtaat tttcaaatat
     3421 tctttcgagt atctttatct gttccttcgt aaacttctta ggcgtacgga aatcatactc
     3481 tttgatctcg tccttgtctt cgtgcgtatc aagcggaaca gagtcgcccg ccatcacgct
     3541 cgacagcata gcgtctatct gttcttgtgt gagggtgtca gccaaaaaat cgccctcctt
     3601 tctcggttat tcttctgatg tttctgccgg tgcagatgtc aatatatctg tagggatacc
     3661 tgtgtatgac ggctcgtact tcggacctgc ctcgttgacg cttgagttgc cttcgcctga
     3721 gttgccgtcg tatctgcttt ccatattgtc gattatctca tttacaacat cgctgttgac
     3781 atctgagctg ttaccgtcgt tgcgctcata cggatcggta tttgcgccgt tcagcctgta
     3841 ttcatacttc aatatatcat caatgacagc ctgagatgtc gtatcgagct gtgcccttac
     3901 gatcattatt tcgacacgtc ggtttgccgc tctgccttct gcggtcgtat tatccgctat
     3961 aggcgtatag cacgcttttc cttctacagt aaacttttcg ctgtcaacac agcggttata
     4021 gtccatatac tttacaacgc tggacgctct tgccgccgaa agatcccagt cgttcacctc
     4081 ggataccgcc ttggcagtat gacctgatac ttcgcaggac tttatatact tgttcacagc
     4141 ctttatgccc ggcataaact tgttgagcac ctgcttgccc tcttctttca gtactgcgct
     4201 gttgccgtca aacattatcg tattattgaa tcggataaag atccttgtcg gtgtctgctc
     4261 aatgtaaaca tactgaccca gatcgttctt ttccgtagtt gtctttaaaa agctgtaaag
     4321 ctggttgaag tcggagggca gaccctctgt ttcttcactt gtagtaccgc cgttttgcgt
     4381 gttcggggga gattcggcgt tgccgttggt atctgcggca ttattcttag gctgttcgtc
     4441 cattacaaac ggatttacat aagtgccgct tgacgtaaag gactgatata tgtattgcca
     4501 ctttgtttcg tctaccgatg atgcggagta cagcaatacg aaaaagcaca gcagtagtgt
     4561 aaccatatcg gcgtatgtat caagccagtt gcccttttct tcctcgccgc ctccgcttct
     4621 tctctttgcc attttctcca ccgcctttct ttatgacgct taacccatat attttaacat
     4681 atttaagtga ctatttcaac acttaatttc ttcgttttgc accggatacg gtcgttttca
     4741 ggcgaacctt tatccgcgac cgcgtcaaaa tgctctgccg actttataac agtgtatctc
     4801 ggtctgcgct ctgagcaaca gctcgcgccg gttcggcact gttctttagc
//
LOCUS       DAWAOG010000087         4822 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2285_length_4822_cov_2.378016, whole genome shotgun sequence.
ACCESSION   DAWAOG010000087 DAWAOG010000000
VERSION     DAWAOG010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2285_length_4822_cov_2.378016"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3934..3943
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1277 a         1189 c         1064 g         1282 t
ORIGIN      
        1 tcgacccggg gggctgtaca gatatctcgg gatagtacgt cttttggaaa tttcggtgat
       61 aattcagttg taacgataca ctcacgaaat tattaacgcc ttgtcggtat cgaaactgtt
      121 tgcggcgttg ttgcttgtct ttattgcacc gtttttacaa tatgttgcac tgcaatttta
      181 atgaaacttg atatgttgag ggattgtgtc acagctcctt tacattatct ctgtgcattg
      241 acaatgcacg ttatcctttt cttataatca tcgcttactc ttctgctgtc gcgtatcttt
      301 tgcgccgcca taagctttac ctttatactt atagggctgt gttcaagata accggtgata
      361 agctccgggt atttcacgca atataccgat aagccccacg ccacagccat atcacggtaa
      421 tatgctccgt aaggaagtgc atcataaata tccagtgcct tttttacggt cggttcatca
      481 gtaaatttcg agaacataag aactatgccg acacgggaaa caaactcctc tttccttttc
      541 atcagcctgt ccgaaagcaa acgcaaagcg tcctcttcac tctttttgac ctttatgcac
      601 gagcagaagc agtcgcactc cgcccagttg tcgatcatcc cggcatagcg tatcacatac
      661 ggcgcttttt cggacagagg cacgtctgca aagccgagtg ccgcaccttt tatgattatc
      721 tcctcaaagc tgtcatacgg catttcaaaa atttccttgt aatccgccct gtgctttttt
      781 gctattgcct taaggtcggg catacgcacg cctatgacct tataattgct tgtatgcact
      841 attttttcat tgaatatacg gaacttttct tccgagaggc tttcaagctc aacaagcagt
      901 tcttcgcgat cgatcattta tacttctcct ccgtttcaag cagaaacttc ttgatatcta
      961 ccccctcttc acccatatag aagcctatgc ctccgcttgc ggggactatc ctgtggcacg
     1021 gtatcagtat cgggagcggg tttcttttca gtgccgagcc gcacgcacgg atcgccttac
     1081 tccctgccgt ttcagccgcc tgcctgtacg tcacacgctc accgtagggt attttctgca
     1141 cggcagtaag tatgctcctg aaaaacggtg tgccgatatt gcttatatca aacggcagtg
     1201 aaaattcttt aagctcacca tcaaagtatg caatgaactg ctctttcgcc atttcgcata
     1261 ttgggtcggg gcagtcggga agcttcatat caaagacgat atttgttata tttctgccgt
     1321 cggtaattac cgttatcgtg cgttcgatcg gcatatcaaa agttatattg ctcattttcc
     1381 gcggtttgcc tttccgctga tctcgtttac cgtaagacgg tagacccttg tccttgcggc
     1441 acacgagccg tccgccttat aggcgatctc tccgtactgc ttaagtatca gcgacagtgc
     1501 aaagagcttt tccgctgtat cggtcatctc ggtcatcgtg ccgtttccga caacgctctc
     1561 atacaggctt gttatactgc cctgcacata gtcgcggcag ttatcaagct caaatgccac
     1621 cttgttattt ctttcgataa gcgcattctt tttgccctca gtcgctgtat ggatataaaa
     1681 atacagcctg ccgtcctttt cctcatatcc gaaattgaac ggcagaacat aaggatattc
     1741 ttcatcaaag aaagcaatgc gcatcacatt tgcatcgttc acaatgccga tgatctcttt
     1801 tctgtcggta atctcacggt cttttcttct catagatgta tgctcccttg tatcgtaaat
     1861 ctactgcaat tattatacga caacaaaaga aacgtgtcaa ccgtcagagg ataacaggct
     1921 gaagctgtat attatcaagc gccgcactta ccgcatcggg gataagcgta aagtcagcca
     1981 cgagcgagca cggctttttc agcggatcgt cctgtccgag cggcacaaaa taatagttgc
     2041 ggcaattcat cagaaaaccg atatttttga atgagcctgc aagtgcaccg tttgtggcac
     2101 aagcgataag caccggtccg gctccgcgaa ggtgactttt caccgccatt gtcacgggcg
     2161 tatcggtgat acttcttgca agctttgcca ttgtgtttcc cgtgcagggt gctaccacca
     2221 ttatatccgt cagtttttta ggacctatcg gttcggcatc gcaaacagac attatcactc
     2281 ttttgccgca gatcttctct gcgcgcttta tatgctcctg agccgttccg aaacgtgtgt
     2341 cggtagtccc cgcgctcagt gacattatcg gaagtatgtc acaacccagg tcgcgcaacg
     2401 cttccatagc cgtaaaagct ttagaaaacg tgcagaatga acctgtcata caaaatccga
     2461 ctctgatacc gcttaattta ctgctcaatt ttatcgcccc tttctctata agtatcaagt
     2521 atctcttcta cggcatcggc tattatctcg cctgcggttt taggcgcggt ttttgcggga
     2581 agcccgcccg catttatgac cgttacgctg tgccccttaa gcgccgcctt atcaattccg
     2641 gaagcagagg caagctctac atagaccgct tcatcgtcaa atgccgcaag ctcatcttca
     2701 ccgatgaccg cacacggaac ggtattgtat atcagcgtga cctcaggcaa tatgccgcac
     2761 agcacatcga agcccaccgc acggcaaccg agcattcttg cctttgctct tttttcacgg
     2821 cttctcgccg caactatgac ctcacacccc attgccgtaa gatatacggc gagctgtgac
     2881 gctatcctgc caaagcccag tatcagcact ttctgaccaa gcatctgtcc gggtgtggcg
     2941 tttatgccga ctgcaagcgc cccctccgca gacggaacgg catttctgag cataacggat
     3001 tcatcgcagt aatgatcctc acagaaaaag ccgttgtcgg cacagaaatg ccgcaatgcg
     3061 ggacttacca ttccgccgat aactacgccg ccgctcttta taagcttctg aagcacggaa
     3121 aaaggaacag gctcgcggca cagcggagcg tttatgctta ttccgtcacg gcttgcgggt
     3181 atgccgagca ccgccgcgtc acacagttca tcggggaaca tatcaagctc acttagtccg
     3241 taggtatata cctcatgctt acgcctgagc ctttcggcag tataatacat acggatatct
     3301 ccgcccagta caaggagctt catttgccaa aacacctctt atctttttat cgctttatat
     3361 gtatacgagc ggcttttttt gcaaaaccgt gtgatgaaca ctgccgcacg gctacatttt
     3421 cgctatcata ttgtatgttt ttgagcactt ttttgtgaag aaaaccaaaa aggcttataa
     3481 agcacttgtt ttttccaaat gtatatgtta ttatatgcaa tagaggctaa cgccccacat
     3541 acttgttgcc gtccggcagc aaatacagaa aggatctaag tgaaatggct actaagaaga
     3601 caaatatgac aggaactaag acacccgtag ctgaagctgt aaagactgaa gctgtaaagg
     3661 ctgccgctga ggttaaggct gatgctgcaa aggcagtaaa ggcagtagaa aaggttgctg
     3721 aaaagacagt tgagaagatc gctgaaaagg ctcccacagc taagaagcct gccgcaaaga
     3781 aggccgcggc gaaaaagtcc gcatccaaga agacaacggc aaaaaagaca acggcgaaga
     3841 aaaccgccgc aaaagcaaag gataaggaat aatattaatg gaaacagtca ccgttttggg
     3901 cgccggactt gccggaagcg agtgcgcatg gcannnnnnn nnntatggta agagaaaagt
     3961 ttgctaagaa ggacgttaca aaggtaaacg aaaagatcgc cattgagatc aaggcttacg
     4021 gtgacaagga atactacatc tacattctta tcgatgacgg taaggtaaca gtagaaccct
     4081 ggggctacat cgacaacgat gtacatatcg atatgcccat tgatgatgtt gttgcagttg
     4141 taaacggcaa gtacgacttt atggcaaagg ctataagcgg cgacttctat gctataggca
     4201 gtgtaacaaa gcttcttaag gcttatcagg cacttatcaa gtaagcgatc ggtctatcga
     4261 ccggatataa gtgttggtac ataaaatgca gacgtaataa tttctgttac gtctgcaaat
     4321 tatataatgg atatgaagaa aacggtaggg aatacatcaa aataaggaca ggtacaaaaa
     4381 tggaaaaagt aaaaaaggca gtaatactcg cagcaggctt cggcacacga gttctccccg
     4441 caagtaaggc tatccccaag gaaatgctca atatagtgga taagcccgct atacagtata
     4501 tcgttgagga ggttatcaac tcaggcataa cagagatatt gatagttctt tcaagaggaa
     4561 aacaggatgt tgaagatcac ttcgaccgca agcccgacct tgaggctcag cttcttgcag
     4621 gcggtaagaa cgaattttac aaggtcgttt gcgacattgc cgaaatgggc aagaatataa
     4681 cctatgtgcg tcagcagaca cagaacggta caggcggtgc ggttatgtac gcaaagcagt
     4741 ttgtcggcaa cgacccgttt gtagttatct acggcgacga tgttatcata ggcgaagacc
     4801 cctgcacggc acagtgctgc ag
//
LOCUS       DAWAOG010000088         4785 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2298_length_4785_cov_2.559831, whole genome shotgun sequence.
ACCESSION   DAWAOG010000088 DAWAOG010000000
VERSION     DAWAOG010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2298_length_4785_cov_2.559831"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    275..284
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1317..1326
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3256..3265
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1069 a         1159 c         1094 g         1433 t
ORIGIN      
        1 ctgtagagtc gccgggagta ggctcatcgg gcttctcggg tttatctgta gagtcacctg
       61 gagtaggatc atcgggagtt ccgggttctt ccttaggaac tgcctttaca ttcatattgt
      121 acagtgaaga accgtccttg taaagcttgt aagctgtaag agcaagagtt gcctgctcgg
      181 tagccatata gttttcttca ccgccgagaa cgtggctgta gccgctttct gtagcatagc
      241 tttcaagtgc atcgataagg gtgataccat caacnnnnnn nnnnagcggc aagagcgata
      301 agtacctgtg atgtgctctc actgccgttt acatatcttg cgttttcgtc aagtgactct
      361 gacaggaact caagaccgct gtcgatagca gactttacct tctcatttgc tgcataggga
      421 gcgagtgcct gtaaagccat agccgtaagg tcaacatcgg cggcagcacc tgcgagtgcc
      481 cagcccttgc ctgtcatatt gtcggtaata taagcgataa gctcatcgtg tgtctttgca
      541 ctgtaatctg ttgtatcaaa tgcgataagc gcgaatattg ccgcattgat accctgagat
      601 ttgatataat ccatatcatc aagaccgctc agtaaattgt aacctgcaac atctgcggag
      661 ttcttaccga gtgcggacag agcgataatt actcttgcat tctctgtagg ctttctgtct
      721 gaaagtctgt ctgagccgtt agcctttacc gcgtcacaaa gctcattgta gtatttatca
      781 gccttgtctg cggaaagctt gcctgctctt gcgagagcga taacagacca ttcgccgccg
      841 atcgactgta gcggatagat cttgttttcg ctgagatttg aaataacatc gtctttgtgc
      901 ttgtcactct ttgcgcctgc ctgctttatc tcatcaatag cggcaaatct ttcgattgcc
      961 ttgtcaagag tttctgcatt tgtaacctta tctcttaaac gctcgggaac ggcggagtaa
     1021 gcgtcctttg cgctgtcgat cttttccttg ctgtcggctg ttacattgcc tatctcatca
     1081 atagcatctg ttgccgcact tactgcatca gcttctgcct gagccagtgc ttcttctgcg
     1141 gctgttaatg tttcgtagct tcctacaagc gtctttgcgt tgtcgctgag cttatcataa
     1201 gctgttcttg ccgctgttat gttgtcatag ctgtcaaggg ttacatcgcc tatagcgtct
     1261 ataagctctt cgacctgctc agctgccttt acatcatcgg ggataagctt cagatannnn
     1321 nnnnnncaga taagcgtata cgggctgttt ttcgccgttc tgtactataa ttcttacagt
     1381 ctttatttca gactgactgc tgagcgctat ttcagccttg ccatctgcgg cgatgcgctg
     1441 attgtttaca taaatattgt cgtctgccgc acctgcaaca gatatgctta cactctcctc
     1501 aacgcctgtg cttacttcat aaacaccgtt atcatcggga gtgagctcct ttactgtctt
     1561 gccgtcatta tctgtgataa ctatttttcc ggggagcgcg gtcgtgccga cttcctcttc
     1621 atttacggta gttcttacaa cataagaaac ctctgtcgat ttctcgttga atatacctgc
     1681 ccagatattg cttgctgtct ggatctttac atagtgaacg ccgtttttga atgtaacggg
     1741 gttaccgtcc tcatccacag cccactttaa gtcaaagccg tttgattcgg cattttcgct
     1801 gtatgcgttt acgttagcac cgatcgtgcc gttcttgaag tagtcaacat aaccgaatct
     1861 tgtcgtgccg acaaaggaag ctgtggagct gtcgccctgt atagtaccct gaacgccggg
     1921 gataacaacg cctctgagtg tgacagtatc gccctttgca aggtcgttca tataataaac
     1981 gctgggtgaa acccatctgc ctgtctgctt tgagccgtca tttgagttgc cctgattgtc
     2041 agtccagctt gttttaccgt cggctgtctt tttgtatgtt acttcgtaat cggtgatggt
     2101 tgtatcttca aagtgctcac tgcctgcgag tgcataccat gtcttgccgt cctcggatac
     2161 gtatacctgt ccgctctcag ccgcacttcc gccgcttaca aagctgttgc cgtatgcata
     2221 gaagtcaaga ccgtaggggt tcgagggatc atcggttatg gggtcgtcat agtaatatgt
     2281 gatataaccg ccgaagttac cgagtgattt tatagtgcct gcgagcgtct gctcgggacc
     2341 tacaccgaag gaaacattgg tgtactggct gttgatgcaa agataatcaa cgaccttgtc
     2401 gggtacgtca tacttgcttc tctttgtagc ctttaccgtg tagctctccg aaagagtgcc
     2461 gttcgatata gttacagttg tgttgccgct cttggcaacg aggctgtaat agccgtcttc
     2521 acccgctgtt gcggcatcac tgcctattgt aactgtgcaa ccgtcagctg ccttgaaata
     2581 tactgtagga aattctgcgt catttgctat aactatatta tagctgagga gtgacgggtc
     2641 aaatgcaggg tataaatcgc cgtaatcact cttaagctct gtaagatgta catcctttac
     2701 agacgcgtca gtctttatga gctttatagt gtagcttgcc accttatctg catcgtcaaa
     2761 cttgtttaca agataatcac cgtcagaaac cgcatcgata gataccgtaa cagtgccatc
     2821 ctcgccgaaa gaatatgcgg gagttgtttc gccgctttta agctcggtgt attcgccgtc
     2881 agcctttgct ctgaaatgaa cgtttgctgt tttaccctta agagtaagtg caacgctgtc
     2941 ggtgttacct aaaatataag atgtgtagct gtggcattct gcgtttgtgc cggtcgtgtc
     3001 ggtcacattg ccgttttcgt catttctgaa aaccgtaccc tccgcaaagc cgttatactt
     3061 tgttgcgtac agctctctgc cgccgggaat aagcgtaatg ccgcctgagc ttgctatttc
     3121 tgccgtgtag tcatagggac gtgttatatt gaatacatac tgcgtcttta tgctctcatc
     3181 gtcaatgtag caaagctcta aaatcagctt ggtaagaccg aaaggaatat tagaaatgct
     3241 gttgaagctg ccggannnnn nnnnncgttt ctctgtgcaa cgccgtttat atcggtatag
     3301 actgcatagc acttcagcag atcattattg aatttagttc cgctgttgat atagaaagat
     3361 gatgtactgt aagactttat ttttatatca tattcgagct tttcggggtc gaaagcatca
     3421 ttgtcccagc cgtcaagtga gaaagccgaa agtgcaaagc tgtcaaaacg tgcggcaggt
     3481 ctgtcgccct cttttacagt taccttgtat gtacttgcgg ctttgccctc gcctaccgta
     3541 acggtgataa catcgccgtc ctttatctct gtaggctttg tcaggctgag tgtcttatcg
     3601 ccgatcttgc ttactgtctt gaaattcttg ttcagtgccg tgggacggaa attgatactc
     3661 tttgtgccga aaggaacagt cagagtatat tcgtaagtct ttgcgccaaa ttcgggggaa
     3721 agcgtgccgt aatcgctgtt tattgcttta agcgatgtgt cagtgccgct ccagtcactg
     3781 ccgaggtcag cgccccagtt catagtgtac tgcatacgga gctcgtcacc gtctgtgagc
     3841 ttgccgttgg ctacagtgta ggcatcaaag ccctcgtttg taaaccagtc gttgagcgtg
     3901 cccatccagc cgctcattga gccgccggct ttttctttaa gtcctgcgat ctcacttatg
     3961 tagcctgcat cagcgccggt ctgggtaaag ccggagcttt caatagcgtc ctttatgcag
     4021 gtcatagcgc ttgaatcttt atcaagcgat acccacttgt cgaccttggt gcctatccat
     4081 gcgggagctt caccgccgtc gaccgcttcg gtaaacgttg tgttttctac gataacacgg
     4141 acgcttccga ggtcatcatc ggcaaatacc gtaaagctta cctgcggtac tgagagtaac
     4201 gcaagcgaaa gcgctaacag taaagccgac acttttgctg taaatttctt cacagttgtc
     4261 atagttttac cttcctttat tcttttttgc cgtaaggctt aaccgtctaa tctgcgtttt
     4321 gccgcttttc ttctttgccg ggagcgttca cgcttggcac gacttttctc ctttgcggat
     4381 actgcgggcg gttctttcag ccctgcggct tcaagcgccc ttgtccacgg acccagcttc
     4441 tgctttataa agcagacttc gcttggagta aaatcggagc gtttgggcag agtgccaagc
     4501 tgttcatact tttcgcaaag catagcgaca gccttgctca atttttcgtc cgccataacg
     4561 tcacctccgt aaacaaatac gaaaaacggc aataaaaaat gccttcacca aaaaaagtga
     4621 aagcacaact gatcttttag tacagcaaac ataagccata cggatatgcc aaaaaaagca
     4681 taaggataca gccttagctg aagatcggct gtacttcttc ctcgtccaag agacaatgcc
     4741 gaaaaatacg ggggtattct gacttgtgag cataggctca gatac
//
LOCUS       DAWAOG010000089         4738 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2320_length_4738_cov_2.162716, whole genome shotgun sequence.
ACCESSION   DAWAOG010000089 DAWAOG010000000
VERSION     DAWAOG010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2320_length_4738_cov_2.162716"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1387 a         1046 c         1077 g         1228 t
ORIGIN      
        1 gcataccgcc atatataaac ataggcgcgg caacagtaac acataaaagc agaacagccg
       61 cgcacagcgc atcaaagctt tcctcagtca taacatcggg cgcaatcttg ccgacaaagc
      121 tctgcattac catcaatgca attacgccga acagtacaag caccgtaccg ccggcgataa
      181 cagcggggaa tttatttctg aatgccttta tctgctctac gctgtaaaac ggcttgatat
      241 tgggattttc cttgacatag ctgtcgtgtc gtatgccgct tactataaat atcgccgtgc
      301 caactgtcac aaacagcatg aatatcgccg cagctatggt ttcatcaagt cccagacccg
      361 ttgttaaaag caatgtagtg acacctgcaa gcacagttgc agtaccaaga cagatagccc
      421 ttgtaaagct gttcatttcc ttatcatatc ctgcggcgtc ttccgtaaag ttttcttctg
      481 cactgccctt gatcagttcg tcaagcgtgc agccaaagta atcgctcagc gtgataagct
      541 tatcagtttc gggaaacgca gtgtcagatt cccactttga cacagtctga cgtgacacgt
      601 tcataaactc cgccagatcc tcctgcgtca tatttttttg ctttctgtaa aactgaatat
      661 ttgttcctaa gctcatatat acatatcctt tccgacagct tttctgtctg tcgtcatatt
      721 agcataattg ccgtgtaagg tctataaact tcgtggtgct tttaaatatt tctatgtaaa
      781 tttgcggttg cattggcttc gtagagccgt tttcgactta tcgcactttg cgccccagat
      841 acagcaaacg gcagaaagcg cacgccctct gccgtaagat tattttgtaa attttaagcg
      901 atcagtctat cggatcacct ttcagttctt cttcggtaag ctcgatagta gatttgtccg
      961 gagcttctac attcatgaat tttcccacaa aagtggataa tataagtcct gcaattatcg
     1021 gcagatagaa tgtaagaaat ctccatataa acgttgagat accgacatac tttgttccgt
     1081 ccgcaccgat aaatatattg ctgaagaaca gtgtgtaact gccctccgcc gcaccgagcg
     1141 cacccggcaa aggcataaat gccgagatca tcagcacgaa cgcctgacac gaaataatgg
     1201 taagataatc cgtacccgac agtccgaagc cgagataaat aacatacgag attgagaagt
     1261 aagccgtcag ctgaattata ttgtaaataa tcagtcgtat gataagccac ggttttttct
     1321 taatgaacac gaaattatca tgatattcgt ctacaatatc ctcaacggac ttgatcttct
     1381 ttatcttatc ttcctcgctc tttataaagc ggaacttgaa aatgtgtatc ttggcaagca
     1441 gctttactgc ccatacagcc agcttcttta cgggcttctt gaaaaatgcc agcataaaaa
     1501 gcagtattat tacgatagta tttatgataa acccaagaat taccaatgcc ataagcggtg
     1561 cgtaatcggt cgagaaattc ttgaatcggg ctataagtac cactgcggaa tatcccgtca
     1621 gcacaaactg atacattatg aatttgctga ggagcgccgt catagcgtgt gacataggaa
     1681 cgccgaactt tgtgaaataa tacacctgca taggctgacc gcccgtagag gacggagtga
     1741 tacaattgaa atactgaccc agcaatgccg tcataaacgt tctgctgaac tttgctttcg
     1801 gatatgttgt tctcagcgcc gcatggatac caagcgcctc cagcagccag tatactatca
     1861 taagactgac cgctataaac aaaaatccga cgtttatctt ccggatagaa ttgacgagat
     1921 tgtcaaatcc gtccacaaaa taaacataaa aaatcataaa tacaaatgca atggcacaaa
     1981 taataaggtt tagcttattc gattttttct ttttcattaa gcctcccggt tttaagtaaa
     2041 ggcattatat tccgaccttt acacacctat aatcccattt tacgataaca ttataattat
     2101 acctcatttc gcagtttttg tcaacgagaa aaaggtcgat tgcgccgtat acactccgca
     2161 gtgctttaaa tcacagaaaa tcagccgtat aaaatgtttt cgccgccacg gcttcttttt
     2221 catcatcact ttaagtaatg gtgctattca atatataaat atcgaaaacg cttgaatttt
     2281 ttgcggcaat ctgctatact atggtgtggc ggatcggaat ttctgtgttt tgcagaattt
     2341 tccacatata acgaaaggaa agggttttta caatgtataa aatactcaaa agacgtcagc
     2401 tcaacgaaaa tgttgtactg atggaggtag acgctccctt cattgcgaag aaggcgttgg
     2461 caggtcagtt catcattctg cgtgttgacg aaaagggcga gagaatacct cttaccattt
     2521 cggattacga cagagaaaag ggtactatta ccatcatatt ccagatagta ggtcagacta
     2581 ctatgctttt atcacagctc aaggagggcg atgcactgct cgactttgta ggtcctctcg
     2641 gcaaggcaac agactttgag ggcgctaaaa aggtatgtgt tatcggcggc ggcgtaggta
     2701 acgctatcgc ttatccttct gcaaagcagt tattcaacga gggtgttgag gttgatgtta
     2761 ttgcaggttt cagaaacaag gatatcgtta ttcttgaaga tgagttcaag aaagcaagca
     2821 ccaatttcta tatcacgaca gatgacggct catacggcga aaagggcttt gtaacaaaca
     2881 agctccagag ccttatcgat gccggcaaca agtacgatct cgttgttgca ataggacctg
     2941 ttcctatgat gaagtttgtc tgtgaggtta cccgtcctta cggcataaag actcttgtat
     3001 cgctcaatcc tattatgatc gacggtacag gtatgtgcgg cggctgccgt gtaaatgtcg
     3061 gcggaaagat caagtttgcc tgtgttgacg gtcctgactt tgacggtcat ttagtaaact
     3121 ttgacgagct gatgaagcgt aactcgacat acaaggaaaa agaagctcac gacagagagc
     3181 actgcagact ctacaaggct gagcagtaag gaaaggacga taagaaaatg cctaatatgt
     3241 cattaactaa ggttcctatg cccgaacagg atcctaacgt aagaaacaag aacttcctcg
     3301 aagttgcaat gggatataca gaagaaatgg caatggaaga agcatcacgt tgccttaact
     3361 gtaagaacaa gccctgcgta agcggttgcc ccgttaacgt aagaataccc gagtttgtag
     3421 caaaggtagc agagggcaag tttgaagaag catacgagat aatcacaagc acaaacagcc
     3481 tccccgcaat cagcggccgt gtttgtcctc aggaaaatca gtgcgagggc aagtgcgtaa
     3541 gaggtatcaa gggcgagagc gtttcgatcg gacgtcttga gcgtttctgt gctgactacc
     3601 atatgaagca cagcgatgca aaggctgtta agccccagtc gaacggcaag aaggttgcag
     3661 ttgtaggtgc aggcccttca ggtcttacct gtgcaggcga ccttgcaaag aagggttacg
     3721 aagtaacagt atttgaagca ttccacacag caggcggtgt tctggtttac ggtatccccg
     3781 aattcagact ccccaaggct atagttaaga aggaagtaga aaatcttcag gatctcggtg
     3841 ttgaagtaaa gacaaacatg gtcatcggtc gtgttctgtc ggtagacgag ctgtttgaga
     3901 tgggctacaa ggctatcttc atcggctcgg gtgcaggtct tccctcattc atgggtatcg
     3961 agggcgagga tctcatcggt gtttactcgg ccaacgagta cctcacaaga acaaacctta
     4021 tgaaggctta tcttgaggac tatgatacgc ctatcatcaa gagcaagtcg gtagcggttg
     4081 tcggcggcgg taacgttgct atggatgccg caagatgtgc aaagcgtctt ggcgctgaaa
     4141 atgtatacat tgtataccgc agaggtatgg aagaaatacc tgcccgtaag gaagaagtac
     4201 atcacgctat ggaagaggga gtcatcttca agaccctcaa caaccctgtc aagatactcg
     4261 gtgacgagaa cggcagagta agagcaatgg agtgcattga gatggaactc ggcgagcctg
     4321 acgcaagcgg cagacgtagg cctattgcta aggaaggaag caacttcgag cttcccgttg
     4381 atacggtcat catgtcaatc ggcacaactc ccaaccccct tatcagaagc acaactcccg
     4441 gtcttgacac caacaagaga ggttgtctta ttgttaacga agacacaatg cagacaaccc
     4501 gtgagggtgt ttatgccggc ggtgacgcag ttacaggtgc agctacggtt attcttgcta
     4561 tgggcgcagg taagcaggcg gcacagagca ttgatgaata tctgagcaag aactgataac
     4621 ggttaaagga tatattgaca ggctgatgcg tttgcatcag cctgttttgt ttacagaaaa
     4681 aggggctgtg atacagccac aaaaaaggaa aagtgagccg aagcgcagaa atttagtg
//
LOCUS       DAWAOG010000090         4732 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2327_length_4732_cov_2.369040, whole genome shotgun sequence.
ACCESSION   DAWAOG010000090 DAWAOG010000000
VERSION     DAWAOG010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2327_length_4732_cov_2.369040"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2417..2426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1139 a         1088 c         1023 g         1472 t
ORIGIN      
        1 gtaggggtgg aagtgcataa cgtttcttgt cgtgccgtcc tcaagctgtt cttcgtcata
       61 agcttctgtg ataactgcaa ggaacagacg ctcaacaccg agcgagggtt cgataacata
      121 aggaacgtac ttttcgtttg tttcgggatc gaagtattcc atcttctgac cgcttgtctt
      181 gatgtgctgt gtaaggtcgt agtcggttct gtccgcaacg ccccaaagct cgccccagcc
      241 gaacgggaac agatattcaa agtctgttgt agcctttgag tagaagcaaa gctcctcctg
      301 gtcgtggtcg cgcagtctta agttctcttc gtttatgccg agggacagga gccacttctt
      361 gcagtagtcc ttccagaagt agaaccagtc aaggtctgta ccgggcttgc agaaaaattc
      421 aagctccatc tgctcgaact ctcttacacg gaagatgaag ttgccgggag tgatctcgtt
      481 tctgaatgac ttacctacct gtgcaacacc gaagggtact tttttacggg tagtacgctg
      541 gatattcata aagtttacaa agataccctg cgccgtttca ggacggaggt aaagctcgct
      601 ctttgagtct tctgtaacgc cctggaatgt cttgaacata aggttgaact gtcttatgtc
      661 tgtgaaatcg gccttgccgc agttggggca ggtgatgcct ttttcacgga tataatccat
      721 catctgctcg tttgaccagc ctgcacagtt tgtgccgtca aagtcctcga taagattatc
      781 cgctctgtga cgtgtcttac aagccttgca atccataagg ggatcggaaa atccgccgat
      841 atgacccgat gctacccatg tctgagggtt catgaggata gcgctgtcaa gtcctacgtt
      901 gtacttgcac tcctgtatga attttttacg ccatgcggac ttgatgttgt tcttcagctc
      961 aacgccgaga ggtccgaaat cccatgtatt tgcaagtcct ccgtatatct cgcttccggg
     1021 atatataaag cctctgccct tacagagagc gaccagctta tccatggttt tttctgtgtt
     1081 tttcatgtgc gaaatttcct ttcctgtttt gcatttaaaa gcagtgcacg gcaacgtatc
     1141 tgcgctgtcg tgcttgtctg ccatataatt gaatacggtt aaaataccgt tttaatacga
     1201 tgaactttta ttcccgaccg ttttgctgta cagaaaggtc ggaacttaca gtgccttaca
     1261 gcttttcgat ccagcccatt tcatcgggga gcttgccgta ctgcatacct gtcattgtat
     1321 cgtaaagacg ctgagagata ggtcctatct tgttgttgtt gataagcata accttatcgc
     1381 cccactttaa gtgacctaca ggtgaaataa cagcggctgt acctgtaccg aatacttcac
     1441 tgagcttgcc tgcatcgtaa gcgtctgcga tttcctgaat tgtaattctc ttctcgctta
     1501 ccttaagtcc ccacttcttg cacagctcta tagatgactt tcttgtgata cccgaaagga
     1561 tagagccctg gagtgcggga gtaacgacct cgccgtcgat cacgaagaag atgttcatag
     1621 cgcctacttc ttcaatgtac ttgcgctcaa caccgtcaag ccacagtacc tgtgagtagt
     1681 tctgcttgtg agcctcgtcc tgaccgatga gcgatgcggc gtagttgccg cctgtctttg
     1741 taaagcccat accgcctctt actgcacgga catagtttgt ttcaacatag atattaacag
     1801 ggtcaagacc cgaagcgtaa tatgcacctg agggtgacat aattatcata aagtaatact
     1861 tgtcgccggg gcgaacgccg aggaacggat cagcggcgaa tatgaacgga cgtatgtaaa
     1921 gcgaagcacc gtctgtatga ggtacccagt ccgcatctat ctttaccagc tcgtggagag
     1981 cctgtaagca gtcgtcaacg tttaactgag ggataacaag acgctctgcg gagatgttca
     2041 ttctcttgaa gttttcttcc ggacggaaga actgaatgct gccgtcagga gttctgtatg
     2101 ccttcatacc ctcgaaaatt tcctgtccgt agtgaagaac catagcggca ggtgaaagcg
     2161 aaatatcccc gtagggaact attcttgcgt catgccagcc cttacccgtg tcatattcca
     2221 taatgaacat atgatcggta aagatcttgc caaagccgag tttggattca tcctccggct
     2281 tttttttcgg attggttgtg agcgtcacag gaatgtcgta catggtcgta tcctccatca
     2341 aagttcggtt tgccgcgaac tctggggcaa aaatgcctcg aatcgtagtg gttctattgt
     2401 atcgccacat gtacatnnnn nnnnnntcgg taaagatgtg accgaaaccg agctttgttt
     2461 cgtcaacggg ctttgcctta ggtgttttag ttttctcaat tttgatttcc attgtaaaaa
     2521 cattccttcc tgaaagcgag tttatcgccg taatttccgc catagcggca gctcgtctgc
     2581 cttatgcaag cgaaataccg tttcctttca gcatcagtat gatgaaaata cagggaaatt
     2641 atgtatgctt gcagtaccga ctgattgtac aaagaaaatt atagcactat tcgccgaaaa
     2701 gttcaatatg ttttttgcac attttgcatt ttttaagcgg atttctttca ttgacaggcg
     2761 gtgctttcat tatggcgaaa ataaatcggt ccgataccgc tttgaggaca tcggaccgat
     2821 catgatattt caggttactg ctggtttgac atttcgtaag attcgatcat cttttttacc
     2881 atctgaccgc cgattgaacc tgcctgcttt gaagtaaggt cgccgttgta accgttatcc
     2941 ttaaggggta cgcctacttc gtttgcggcc tgcatcttga tactgcttaa tgctgccttg
     3001 ttctttttat tagtactagc cattgttgtt ttcctccgta attttttgca ggtccttttg
     3061 accttgcaac attattatgt tcggagaaat tttaaatata agatgaaatt tatggtgatt
     3121 agattacgtt tttcctgtcg cttattataa ctccgtccga tatctcgatt attcttttac
     3181 agcgtgacgc gatatccttg tcatgagtta ctataatgac ggttttgccc tcgcggctga
     3241 gcgtttcaaa aatacgcatt atctcatcgg ctgtatcgct gtcaagagca cctgtgggtt
     3301 cgtccgcaag tatgatctca ggctcgttta ctatagctct tgcaatagcg gttctttgct
     3361 tctgaccgcc cgaaagcttg aatacgggtg tcgatatctt atcggacagc cctaccttct
     3421 gcaaaacaag ccttaccgct ttttctttgt cgatctgttt ttttgaatat atcagaggta
     3481 tgcgcacgtt ttgctttacc gtgtattttt cgaccagtgc aaagtcctgt attacaaagc
     3541 cgataacggc attgcgaagc ccggcttgct gtttttctcc gacagaagat acatttttgc
     3601 cgtttatagc gtattcgccg tctgtcggct tatcaagcag tccgagaata ttgagcaggg
     3661 tggattttcc gcttccgctt tttccgatta tcgcgacaag ctctccgctg tttatatcaa
     3721 agcttacgcc tttcagcgct ttgacctcag cctcgcccga cgagaaattt cttgtgagat
     3781 tttttacgct tatcatagct tttcctcctt cacggcgatt ataagtgact tctttttcag
     3841 tgttgttaca acaggtataa gagaaataat tacggagatt attccggtaa taattatcgc
     3901 aatagttgct gtcaacgtat gcaggattac agccattatc atgccgctta aaaataccac
     3961 gaaaaatgca cctgccccga cccttaaaat aatatcgttt attgtagcgc cgcatatgac
     4021 gtgtataaga agctcggata tattttttct gacatatgtg agcatcgttt cgataaggct
     4081 cagaagacat agcacaagta ttatcgcccc gattatgatc ataatcatgc tgttttgtaa
     4141 aaatgtatcg gagcgctgtt ttatcatatc gtttattgaa atgaaattat agccgtaaat
     4201 gccgagctct gcggcttttt cgttgatcgc cgaaagagcg tttttatttt ccgcgaaaat
     4261 aagctgtgtg gggtaatcga taccgttttc tttaagaaat tccgtgctca ttatcggcat
     4321 atatactgcc gtgtcaacag atgttgcctc catatcgctg tacatccaat atgtatattg
     4381 tccgggcttc agaatgccga ctatttcgta tttatcaaga tatttatctc ctaccgaata
     4441 tccgtctgcg gctgtgcttc cgactacaac gggaatgttt ttgccgtcac cgccatattc
     4501 atcatcagag aacagcctgc cctgttcgac cgttatattg taataatcaa aaaaagcggg
     4561 agttatcccg tacacatcat aaatatctcc cgatatgtta tcgccgtata caagactatc
     4621 aagatatgca aagtctacgg ttttgtagtt ttcgcctatg taatatgaaa gctcgtttgt
     4681 ttcgcccgaa attgccaccc tgatctcatc gtccgttttt gatttaacat aa
//
LOCUS       DAWAOG010000091         4718 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2336_length_4718_cov_2.528201, whole genome shotgun sequence.
ACCESSION   DAWAOG010000091 DAWAOG010000000
VERSION     DAWAOG010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2336_length_4718_cov_2.528201"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1403 a         1141 c         1086 g         1088 t
ORIGIN      
        1 aaaacacatc gtcagacaac agctcagttg atgatacctc gtcagacaac agctcagttg
       61 atgatacctc gtcagacaac agctcgaccg atgatacatc atcagacaac agctcagccg
      121 atgatacatc atcagacaac agctcaaccg atgatacctc gtcagacaac agctcgaccg
      181 atgatacatc gtcagacaac agctcagctg atgataccga gcctgccgca cgatccgaaa
      241 aaggtgacgc tatagccaaa acggcatacg atatgctggg taagccgttc accgacggcg
      301 gatacaaacc ggaaaccggc ttcgataact ccggacttat atactatgcg ctgacaagcg
      361 cgggtgttga gtgccccaga ggtcttactg cacagaaaga catgggtaaa gaggtcagtc
      421 tttccgaact gcaaaaaggc gatattgtct ttataaaaga cggtgagtct ttcttcgcag
      481 gcatatacac aggtgataac acaatagttt tcagcccttt tcccgggcag aaagtgcgca
      541 ccgccgatct caggtcgata tactatatcg acaacttcct taaggctgta agagtggatt
      601 aacgctttta ttgcacaatc atcacaaagc tttttcagca tcggataata cctacacaat
      661 agcttcatac cgctttacaa cattacaaca gcttctcaga ctacatcgca gtagcattca
      721 gccggaactt tcatagcatc ttcaaatatt tatacagcat tttcacatcg cttcgacagt
      781 tttcttaact tccgctgcat tgcagcaaat tctcctctct tatatatttg cccctccgct
      841 tttgcggagg ggcaaaatct ttttaacgaa aacccgattt atcttgattt tcactgctca
      901 aactgctaca atataatcat acaaattcag ctataaagcc gaaaggaaaa gtaaaatgga
      961 aaaacgcaca gtcttattca atgataactg gaaatttgct cttaccgagc ccgacagcga
     1021 tgaaaaggct cttcagaacg tgcattggta taatgtcgag ataccccacg actggcttat
     1081 cggagatacc gcaaatctct ataaatccgg ttgcggctgg tacgaaaagc gcttctctgt
     1141 agacaagctc gatccacagg actgctatat cctgatattt gacggtgttt atatggatac
     1201 taccgtttat gtcaacggaa aggcggtcgg tgaatggaaa tacggctata cctcgttcag
     1261 ctttgatata accgacagcc ttatcgaggg cgacaatcgc attttagtga gagtaaatca
     1321 taaagcgccg aatacccgct ggtacagcgg agcaggcata taccgcaacg tatggctgag
     1381 aaagacctca cgcaaccata tcgtacagga cggcatatat ataaacgcca gacccaacga
     1441 cagcgaccac agagagcaag gaaagtggaa ggtaattata cagacggagc tgtcgatgca
     1501 gacctgcgga acgcttgcgt ttgatgtgac cgacccgaca ggatgtgaag cttacagttg
     1561 cgaacgcaga gtatgcggaa aaagcgacaa gctggtgttc tatatcgacg gacctaagct
     1621 ttgggatatc atcacacccg atctttactc attatcggtt gcactgtatg ccgaggacgg
     1681 aacgcaatac gacagtgttc agactaaatt cggtttcaga accacaaagt tcgacccgaa
     1741 agagggtttt ctgctcaacg gtatccgtgt taagctcaac ggcgtttgta tgcaccacga
     1801 tctcggagca ctcggcgcgg ctgtaaatta tgatgcaaca tatcgacagc tttcaaagat
     1861 gaaggaaatg ggtgtcaacg ctatccgtac aagccacaac ccgccgtcaa aagaacttat
     1921 ggatatatgc gacaaaatgg gactgctcgt agacagcgag atattcgata tgtgggagct
     1981 tgcaaagaat gagaacgact atcaccgttt ctttcccgaa tggtataaaa cagacgttgc
     2041 cgcatggata cgccgtgacc gcaaccaccc ctccgtcatc atgtggagca taggaaacga
     2101 aatttacgac acccacaaaa gtccgcgagg tcttgaggtg gcaaagatgc tggctgaaga
     2161 ggtcgaaaag aacgacccta tgcacaacgc tccgcctact atagcgtcaa actatatgca
     2221 gtgggaaaat gcacagaatg tagctgactt ccttaagatc gccggctata actatgccga
     2281 aaagctgtac gacgatcatc acgaaaagca ccccgactgg gtgatatacg gcagcgagac
     2341 cgcatctaca gtccgctcac gaggaattta ccatttccct tacgaaacgt cacttcttgt
     2401 atatgatgat ctgcagtgct ccgatctcgg caacagtgtt gtaaactggg gcgcttcgcc
     2461 gcttaattct tatgctatgg atactgccgc cgattattcg atggggcagt ttgtctggac
     2521 cggctttgac tacatcggcg agcctactcc ctacaatacc aaaaattcat atttcggcat
     2581 agtggatacc gccggttttg aaaaggactc gttctggttc tataaatcgg tgtgggatat
     2641 cgcctatgaa aagcctttta tccacctttc gccctgctac tgggacttta acgagggtca
     2701 gatgatagat atcatcgcat actcaaacct acccgttctt aagctccgtt acggcggaaa
     2761 aacatacgac agcgaaacgc ttgaccacaa gagcgagggc aagctgtgcg cacatttccg
     2821 tgtgccttat cacaagaagc tgccgatagc cgtaataggc tattataatg aaaactgcac
     2881 gaacgacaac tatgcggtcg aggaggtgct cttcacccct tatgaaacct ataagctccg
     2941 tatgcgcagt gataaaaatg agataaccgc cgatggcaga tctcttgcat ttataacaat
     3001 aacagcagag gatataacag gacaggaagt acagaacgcc aacaacagga taaaattcac
     3061 tgtcagcggt gcaggcagac tgctcggact ggacaacggc gacagcaccg actacgacag
     3121 ctacaagggc agtcaccgta agctgttctc aggcaagctc cttgcgatca tcggttcgac
     3181 atttgaaaca ggcgaaatta cagtcaccgc cgagagcgaa ggtctgcagc cgacatcgtt
     3241 agtcattaac gcggtcgctc ccgaaaccgt ccccgagggt gtaagtgttg tgaccgaaaa
     3301 tgcattcccc gcagtcacaa ctgcgtacac caaagaaata ccggtccgca agatagagct
     3361 taccgacaac ggcactcata agctcggcaa ggacaacgat acagcgatgg taagcgttaa
     3421 gatacatccc gaaaatgcaa cgttccgcga tatagagttc aagtgctgtg cagacaacgg
     3481 cgttgagata agctttgcaa gggtaacaga ttttgacggc agtacagctg tgttaaaggc
     3541 gttcggtgac ggtaatttcc gtctgcgcgc ctattcaaag aacggtacgg atatccctaa
     3601 ggtgatctcc gagcttgaat atactgtcac aggacttggc aaggcagaaa aagatccgta
     3661 tatctttgtt gccgcctgtc tgtgcgattt ctcgaatgtt cccgtcacaa cgatagacag
     3721 aggctcactc ggcggattta acggcagaac cgtagtcggc tttggctcga tagatttcgg
     3781 cagcggtaca aagcatatca cattgagcgt aggcaacagc ggcggcggtg aagattaccc
     3841 cgtagagcta tatctgggcg atgcggataa cggcggcgaa tacatcggca cattcaatat
     3901 aaagaataac ggcggctggg acagggcgta ccctcaggag ttcgatctcc cgcgcagaat
     3961 aagcggaata catgatatca gcttcgtcat aaatgattcc tgcatattcg gcggctttga
     4021 atttgcaaag tacgaccgca cctatgcaga gaatgcggca gcggacaacg ataaccttta
     4081 cggtgacgat tataaggtaa acggttcatg cgttgaagag ataggaaaca atgttgttat
     4141 cgaatttaac gatcttgact ttgcagacgg cacggacaag ataaccgtta caggcagaac
     4201 accgcttgac aactgtacta tacagctcag ggtaaacgat gagaacggca atcagacgac
     4261 ccgcctgctt gagttcccgc acgcagacgg gtatacaccc gtaacattcg atatcgagcg
     4321 tatctgcgga aagaataata tcgcttttgt attcctgccc ggtacacagt tcgatatggc
     4381 atcgttcaga tttataaaag cggaagaata aaacactcag gagctgtgac acaattcctc
     4441 agcttatcag atttcattaa aattacaatg cagtatattg taaaacgtca aaacaataaa
     4501 tatagtgccg gcatcgctga aaacagcttc agcactaata aggtgttaat aatttcgtga
     4561 gtgtatcaat gcaactgcat tatcaccgaa acttccaaaa acgcactatc caaagatatc
     4621 tgtacagtcc cccgggtcga acagagcgga gcgtcccgct tataatggga tttgaaacgc
     4681 aatcgtttgt atgtcttatc gccaacaccc cattatgg
//
LOCUS       DAWAOG010000092         4705 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2347_length_4705_cov_2.681505, whole genome shotgun sequence.
ACCESSION   DAWAOG010000092 DAWAOG010000000
VERSION     DAWAOG010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2347_length_4705_cov_2.681505"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2141..2150
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3934..3943
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1400 a         1022 c         1044 g         1219 t
ORIGIN      
        1 agcgccttcc tctctcccct tgggttgtct ccctcacaca tagcagcaac gttattaaat
       61 agaatagcga acccgagaag tgctatccaa cgaatagaac tatcggcagc ctgctatcag
      121 agtataagca tcgaacaata taacagaagc atacacctcg aatatcttta acatttaatt
      181 cccccatatc cgtataagtc gggaaaacag cgggaataat aacattccct catctttttg
      241 tagtcaaacc actattattc gctcataaaa atgtaaccat acgccgatta ttcgctcatt
      301 ttttgtgact aaacgacaaa agttcgctca tgaaagtgtg aggagaggtt gacttttcgc
      361 ttaacaatgc ggctttacgg gtagaataaa aaatataata aaaattttca aaaaacctat
      421 tgacaaaacc tatctgatta gtgtataata taaacatacc aaacagatag gttttgagag
      481 taagaaaacc tatccaagtg tgaataaaag aaaggaggca catttgatga gtatcgtcgt
      541 aaaagcctta cggcacaatt tcaggaacgg acgaatgtgc cgctgtatag cataagtaaa
      601 gtgcttaaag cacaccccgg aaaacaaaac taaaataaga atttaacagg ctataagccg
      661 actgaaagaa agaggtattt acaatgagct atacatatga taacaactac agatttgaga
      721 cgatccagct tcacgcaggt caggaaactc ccgatcccgc atcagacgca agagcagttc
      781 cgatatatca gacaacatca tacgttttca agaacagcgc acacgctgcc gcacgtttcg
      841 gtcttgctga tgcaggcaat atatacggca gacttacaaa ctcaacacag ggtgcttttg
      901 aaacaagaat tgccgctctt gagggcggtg ttgccgcact tgctacagca tcgggcgcag
      961 ccgctataac atacgctatt caggcgctcg cttataaggg tgagcacata gtagcacaga
     1021 agacgatcta cggcggcagc tacaacctgc ttgcacacac acttccacag tacggtatca
     1081 gcactacatt tgtagatgcg cacaaccttg aagaagttga gaacgcaatt caggataaca
     1141 caaaggctat ctaccttgaa acgctcggca accccaacag cgatatcccc gacattgacg
     1201 ctatcgcaga gatcgctcac aagcacggac ttccgctcgt aatagacaac acattcggta
     1261 ctccttacct tatccgtcct atagagcacg gtgcggacgt tgtagtacac tcggcaacaa
     1321 agttcatcgg cggtcacggc acaacgctcg gcggcgttat cgttgactcg ggtaagtttg
     1381 actggaagaa gagcggcaag tacgctccca tcgcagaacc caaccccagc tatcacggtg
     1441 tatcctttgt agatgctgta ggtcctgccg cattcgtaac atatataaga gctattcttc
     1501 tgcgtgatac aggcgctaca atttcaccgt tcaacgcttt cctgctttta cagggcacag
     1561 aaacactctc gttaagactt gaccgccata ttgagaacac aaagaaggtt gtagagttcc
     1621 ttgctaacca cccaaaggtt gaaaaggtaa accatccttc tctgcccgat caccccgatc
     1681 acgctctata tacaaagtac tttaaaaacg gcggtgcttc tatcttcacg ttcaacatca
     1741 agggcggtca ggaagaagct cataagttca tcgacagctt acagatattc tcactgctcg
     1801 caaacgttgc agatgtaaag agccttgtaa ttcaccctgc tacaacaact cactcacagc
     1861 tgacagatga agagcttgcc gaccagggta taggcagaaa cactatccgc ctttcgatcg
     1921 gcacggagca catcgacgat ataatcgccg acctctcaca ggcatttgac aaagtctaag
     1981 tgccgatgcg gcacggcaaa ttccgccttt gatatagttt ctgctatgct tagcatggcg
     2041 caacggcggg atcaaagaaa tcaagattta caatgatttg atatggcaaa atcgttcctc
     2101 cccagattaa cctttcaatt caattaaagt ggcgcattgc nnnnnnnnnn cccccccgcc
     2161 ggtaacggag gcaaagctta ttgccgatcc ccgcaacggc gaacttgaag cggctcacag
     2221 ccgcactctc ctagattatg tgatattccg aataacgagc cgacagttaa aatgctgtcg
     2281 gctttcgttg gtattcagaa aggactaaaa tgaacaataa ggcagtaatt gcaatgagcg
     2341 gcggcgttga ttcaagcgtg gcggcttacc ttatgaaaca gcgcgggttt gactgcatcg
     2401 gtgttacaat gaagctgttt gcaaatgagg acataggcgt gtcgagagag catagctgtt
     2461 gctcgcttga cgatgtcgag gacgcaagga gcgttgcgta ttcgcttgat atgccgtact
     2521 atgtttttaa cttctcagac aggtttgaaa ccgatgtgat agaccgcttt atacacgctt
     2581 atgagaacgg cataacacca aatccctgca tagactgcaa caggtatctg aagttcgaca
     2641 agctttatat gagagccaag gagcttgacc gcgactatgt agtaacgggt cactacgcaa
     2701 gggtcgaaaa gggcggaaac ggcagatatc tgcttaaaaa ggcgcttgat gataccaagg
     2761 atcagagcta tgtattgtat tgccttacac aggaacagct cagccacacc atatttccgc
     2821 tcggagagat gagaaaatcg caggtgagag aaattgccga gacccagggc tttataaatg
     2881 cgcggaaagc cgacagtcag gatatatgct ttgttcagaa tggcagctac gccgacttta
     2941 tagagcagta cacaggcaag aattatccgg acggcgattt tctcgataca gatggtaacg
     3001 ttatcggcag gcacaagggc gtgataaggt acactgtagg tcagcgtaag ggactgggga
     3061 tttccgcacc cgaaccgctg tatgtaaagg cggtggaccc tgtaagcaac accgtaacgc
     3121 tgtcatataa cgacggactg tacagcagtg tggttaaggc gggggatatc aatcttattt
     3181 ccgtgccgga gataaagggc gaaatgcgcg ttaaggctaa ggtacgctac cgccacaccg
     3241 agcagtgggc taccgttacg caaaacggtg atactctcac ggcggttttt gatgagccgc
     3301 aaagagcggt cacctgcggt caggcgttgg tgctgtatga cggagatatc gtggtcggcg
     3361 ggggaactat aatcgaggta gaataatatt cggagtattc ataaaagcac aaacggtctg
     3421 aggagtataa aattctcaga ccgtttttat ataaagtaac ataaaatgct tgttaacgca
     3481 tgaaaaatat gttataattt gtatacaaag cgagttctcg tgtgaaaccc gaataattga
     3541 ttattcctgc ggtttataga ttcctttcag tgaagaaggc aaagtaattt cagcagtccc
     3601 cattcacaaa taagctatta tgttttggca aaggagtaga taaatgaaat tgttagaaaa
     3661 atgcaatttt atattactag ctcttttggt atgggcttta acggttttat acaacggcat
     3721 attcacgaat tatttgtcgg atttatttct ttctgtttct aatccgtctt ggtttgggct
     3781 tattgtgcat acgttaatac aattactgat catatgcacc ctgacaataa ttactataaa
     3841 atgccttaaa cttaacgtaa aacagctgtt gttttcaatt ccgattatgt atttgctatt
     3901 tgcaatttat tcaccacccg ccttatattt gttnnnnnnn nnntgtttgt attcacgggt
     3961 gggtggaacg gtttttggca acaatctcat cctgcaatgc tattctggtt agcttccatt
     4021 ttcataactg tgcaatacgg tatcgttatg ctgataacga cgctggttgc ttgcagaaag
     4081 aataaggcag atgatgcaaa ctgaagcttt ttcaatgttc gtaaagcatt cgccccccac
     4141 tcgcaactat cttattattt gtcagcatag attgacggaa aggaaagggt aataatatga
     4201 aaaaacaccc gttgcttttt aatttcattt ttacgatggc aattcagata ggcgtatatc
     4261 tgatcgaaat atttgaggtg cagataatcg agcctatgct tcaagcgatg ggagtgagaa
     4321 gtgaagggat attgccgcct acggcagtag taagtctcat tgttttcagt gcagtattga
     4381 tagcggcggc aattgcggca ggtgttattc ggtcaaaggt ttatttctgg ataccgtcat
     4441 tccttttgat atttattggg tttgcaattt attatcctga taatcttgca agaatcaagg
     4501 aagacaacat aatcaatttg ccgtggatag cggaacattt aggttatggc agtataatgt
     4561 attctgctat ctgtatagca gtagctaagt tccttttcca gcttctgccg tatacgatta
     4621 cccgtataat aaagcaaaag cttgaagcga gaaacaatga gatccgtatc aaaaaaggta
     4681 aaggcgcgac cgttacacag ctttc
//
LOCUS       DAWAOG010000093         4688 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2355_length_4688_cov_2.178286, whole genome shotgun sequence.
ACCESSION   DAWAOG010000093 DAWAOG010000000
VERSION     DAWAOG010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2355_length_4688_cov_2.178286"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2347..2356
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1388 a          941 c         1074 g         1275 t
ORIGIN      
        1 aaactatatc gactaaactt cactaaatta cgccaaactt cacagtttta ttgacatcgc
       61 aatttttttg ttatataatt caaaaaaatg cagactaaat tggcaattcc cattcaaaga
      121 ataactagtg ataatacaat aaaaatcaga gctaagtcat gtataggaga aaaaatgaaa
      181 ctcgaaatca accggctcac aaaatcctac tcaaaaggca aaaaagccct cgacagcttt
      241 accctcaccc ttgaaaaagg cgtgtacggc atcttaggtc ccaacggtgc gggcaaatcc
      301 acgttgataa actgcgtaac cggtataata aagcctgaca gtggcagtat tgctttcacc
      361 gacggcgaca gccacgatta tatgtcgcac ctcggctttt taccgcagaa tcaggacttt
      421 tatcctaatt ttacggcaag agagcttatc ctctactcta tggcactgaa aaatgtaaag
      481 gtcgataatc ccaaagccta tgccgacggg attttaaagc gtgtaaatct gtatgacgac
      541 cgcaacaaaa aggttggcgc acattcgggc ggtatgaagc aaaggctcgg catagcgcag
      601 gcacttgtag gcgagccaaa ggtggttata tttgacgagc cgacagcagg acttgacccc
      661 aaggagcgta tacgcttcag aaatgtaatc tcatcgcttg cgaatgataa gatagtaatt
      721 ttagcaactc atatcgttac cgatattgct tttattgcca agaatgttgt acttatcgat
      781 ggcggaaaga tgataagctg tgcctcacaa agtgagcttt gcaatgatat ttacggcaag
      841 gtctgggagg tgctcaccga ctacgaaaca ggtatggagc ttatgcggac aaagcgcgtc
      901 agcaatgtta taagcgagga cgacaggttc aatgtccgtg ttgtaagtga cgagaagcca
      961 caccacgacg ccgttaatgt tcagcctacc cttgaagatg tgtgcattta tcacttcgga
     1021 gagataggtg aatgatatgc tgaaatacga gcttaaaaag tacatactga agccgtcact
     1081 ccttatctgt gttatcattc ttgttctttt aaactttgta aaggtttttg agctgtatta
     1141 ttacatggga ggaggcagag ccgtcctttc gggaaacgct gtacaaggca tggatatact
     1201 gtacgataaa tacagcggaa agataaccga tgaaaagata aacggactaa aaaccgagct
     1261 tgctaatgcg gagcagatgc taaaagagta cggtataatt gaagagccga ttgagggtac
     1321 atattgcggc tacccatacg gtgatgtaaa tttattcaag gatcttatat cgtcctacga
     1381 atacgctatt ctatactcaa ataaaagcgc agaaataacc gaaaatgcaa gggcaaacgc
     1441 cgagttctac agcgataaga gccggtacga ataccgtctt tcaaagctgt atgaggattg
     1501 ctataaaggc aggtcgctcg acggctatta tcagtccgag ggacacaagg ctatcttcga
     1561 ctataagttt tccgccttgc tgtcaatgtt tatcatagtg cttgcaatat ctccgatagt
     1621 ttcaaaggag tatgttatag gctacaataa gcttatatct tcatcaggaa aaagagggtc
     1681 ggttatgctt gcaaagctga cagcggcggc gatatttaca tttgcggtta cacttatact
     1741 gtttgcttcg gatatggttt attttcagct tatctacggc tttgacggat tttccgcacc
     1801 gatatacgcc ttgcaggaat ttgaaatgtg cccatttaat attacgcttt tcggcgctct
     1861 tgtgataacg ttcgtattaa gacttgtgtt tctgcttatg tttacattcc ttgtgacggc
     1921 agtatcgtcc ttttgcaaaa acgatataat ttcaatggtt gtaagcgtag cggcgggatt
     1981 tctgcttgta atcggctcgg agctgctccc cggcgtgctt aaccccacga cgcttattgg
     2041 tccgacagag cttttcacaa atatgaatgt ctgcaatatc cttgatttcc ccatattcaa
     2101 tgttgttgta actttatctg aagtaatact tctcgctgtc gtctttgctg tcgtgtctgt
     2161 acggaggggt atgaaatgct gttaaaaaat gaactgaaaa aactgtacat aaagcagtac
     2221 ggacttattg tactgctgat agtctttata gtaaaactgc tcacaagtgc ggatttatac
     2281 aaggcaagct acagcgatat gtcgtacgaa cagcagaaat attatctcga atatatgcag
     2341 gagtacnnnn nnnnnngcta tactctcgct atacagcgag gcagaagccg caaagcagct
     2401 gcaaagcgat atacttgata aaaacagaaa cggagactat gccactcccg aagaatttac
     2461 aaacgcgatg cgggagatac ccgacataat cgaaaagtat gacgctataa agctgcttta
     2521 ttcaaactat gagagagtaa gcgcagaccg tgaaaatctg cttatgctcc cgtccggctc
     2581 aaacgccatg acaagcggaa tagagtatct gtttattatg gcaatatgct atatctctgc
     2641 ggcaatgtgc tattacgaaa gaaagatgaa gccgcttatc gttaccgccg caaacggcag
     2701 acgctcgggc gggtacaggc ttatatcctt gttttcgctc atttttaccg gctggctcgg
     2761 actgtttatt atagagctta cttcattatt tatcgttatc ggagcggaaa acctcggctg
     2821 tggcgtacag tcacttgaag cctttgagaa caccccgttt ggcagtctgt ctataatcgc
     2881 aatgtttata gttattcacc tgacaaagct gctcggctac ctgcttattt cggctgtatg
     2941 cgtactgctt tgtacgctga caaaaaatct gccgctgtcg ctgtttgtgc ctatggcggt
     3001 cacttgcgtg tgggtttatc ttttcggccg gaataatgcc gtgtattatt cgccgttttc
     3061 acttgtgctc ggctcgccgt attacacggg agattgttat gtaaccgagg gcagacttga
     3121 aattctgctg tattcatgcg ttcctgccga acttctggtt atgctgataa cgatagctgt
     3181 tattgtgata gcggttactg cggcagtata tatcggcagt ataaagcgct gtcgtccggg
     3241 aaaaaaggca gtgatttcgg cagttgctgc ttcgctgata ctgctgttgt caggctgttc
     3301 tcagagcacc gccgataaca cggcggcaga cggaagatac ggttttgctt atgacggtga
     3361 aggatattac gtacttagta ccgagactga cgatgaatac aatataattt ctcaaaggat
     3421 aatcagatat gacgataagc tgaatctttc agaggacgat atactgcgaa atataacctg
     3481 tgacggcaga gttgattata tgcttgcaag cgacgggtat ctttattata ccgaaagctt
     3541 tcagaatggc ggcacatata ccgataatgt gtgcagaata cggcttagcg attattataa
     3601 agaaatcgtt cttgccgcgc ctgatgccca gcggctcagc cgctatcttg acctgctgac
     3661 aatatggtcg ggtgacatcg atgattacag ctacaacggt atgtgtaagt atcagaataa
     3721 tctgtatctg cagactaata actacaaggt gtttgtactc gacttgaata cgggcgcgcg
     3781 ccgtctgctg ttcagcgaga attatataaa cggggatatt tccgttattg acggcaaggt
     3841 gttctatctt aattccgacg gcaatccggt ctgctttgac aatgaaaaga agattatcag
     3901 cgaaagaatg ttctatgaga ttgcgtctga cagagaatat atttactgct cgaacaattc
     3961 tgtcacttat cggtataagg cgagtgattt atcggaagaa aagtttgccg acaagggaga
     4021 agcgtatatg gcggacagga gctgtgtgta tttcggcgac ggaacatata tggacgctta
     4081 cggaaaacag gtggaaatat ctcaggcgaa ggacggaagt atttttcttg caaacggcag
     4141 agtaatagtc aaaaacagtg acggaacatt agaattttca gacaaataac ggaggaaacg
     4201 taaatgaaaa aactaattat tgcatcattg cttgcaatag ctcttgtttt ttcgctctgt
     4261 tcctgtgcgg gaacaagtga aagtgacaca cagagccttg tgttaaatcc atttgaaaca
     4321 tcggagaacc tcaacagcga aactcatacc gaggacgagc gctatgagct ttatgacaag
     4381 taccagagcg gcgctctgca atttgacttt tcagaaatga acgacgcgca gaatatcgaa
     4441 tataacggcg aaaaaataaa tataactttt tcggcaaatg ccgcaaataa ccgggaagac
     4501 ataacgatag gatatatggc gtttatcgga ggaataccgc aggttatatc cgtaaacggg
     4561 agtgaagaag ccgaaactgt aagcgtaaac tttcctaaag atgaaattac aaacgtatct
     4621 atcaacataa aacctcgcat tacaaaggag cttgaagata aaaaagaact gaatttgcag
     4681 attgtttc
//
LOCUS       DAWAOG010000094         4654 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2375_length_4654_cov_2.090672, whole genome shotgun sequence.
ACCESSION   DAWAOG010000094 DAWAOG010000000
VERSION     DAWAOG010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2375_length_4654_cov_2.090672"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    457..466
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1170 a         1151 c          946 g         1377 t
ORIGIN      
        1 cctataccag tacgataaat tcgtttccga gtgcggggag tatgttcttt atagcctgcg
       61 gaagcacgat aagccacatt gtctgacctg caccaagacc cagcgatctt cctgcctctg
      121 tctgaccctt gtcgatagac tcgatacctg cacggacaat ctccgcaaca tacgcacccg
      181 aatttatacc aaagcacaaa actgcggcaa ctattgcgtt tgcgcctctg tagttgaaca
      241 gaactgcgtt tatgagcaac agctgtacaa gtacgggtgt acctcttatt acgaatacat
      301 acaggtcgca tatcttagct atccagccga tacctctcct gcctgcgcat aactgcttta
      361 ctactgccac gaatgcaccg ataagaaaac cgagaataac cgcaaacagc gatatgagca
      421 gtgtgtttcc gataccgctt aaatagaatt tataacnnnn nnnnnngtgc ttgagtgtga
      481 attcgtcaac cttgccttcg tcaatcagct tctgaagaac cttgtttacc ttttcttcaa
      541 gctctgtgtt gcccttcttg aaagcgaatg cgtacttgtc tgtgaatacc tcgccgtcaa
      601 ggatctcgaa gttgtcgttg ccctttacca tttcctttgc aggaagctcg tccataacga
      661 tgcagtcaac cttgtcgctc tttaagtctt ctgccgcctg aacatacttt gtataacgtg
      721 agggttcctt cttaagatcg tctgttacat atgtatcggc aactgttgac atctgaacgg
      781 caatctttgt gtcaccgccg aggtcgtcgg cactctttac tctgtttgct cccttgagta
      841 caacgataac ctgctttgac ttagcgtatt cgattgagaa gtccatagcc tcctgacgca
      901 cgggagtaac cgagatgcct gctgctgcaa agtcaacctt gccctgctgt acggctaaaa
      961 gcgcaccgtc aaagtccatt gactggatct caagctccat accgagctcc tttgcaactt
     1021 cgtttgcaat atcaacgtcg atacctacaa catcgttacc gctcatatat tcgtaaggag
     1081 cgaaaccggg ctctgtagcc ataatgagct tgccgcctgt tgtgtttgac tgacagcctg
     1141 ccatagcaag accgcaggta gcggcaacga gaatgcctgc aattattctt ttgatcttca
     1201 taaaaatacc tcttttttaa aattctttct gttttagtat gcgctttctg cgctatcgtt
     1261 aatattatag tgcatttctt tacaataagc aagagttttt cgcttttttg tgtaaaatac
     1321 atataaaatt ctgcggtatt gagggttaca aagccggaaa aatgcgaaaa atcgctttca
     1381 tatcaaaatg atccgtcata tcagaacgcc gcggctcaca gccgcaaaaa ggataccgca
     1441 agcctgactc atttgcagta tccttttaat taagcctgaa ttgaatgtgc aacagcatat
     1501 taagcccgcc ggtaacgcaa ccgtcgtcta tcgccaacag tctcagcggc gaatttgccg
     1561 caggtcacac ctgaacggcg aattgtcagc ggcgccccgc cgcccgctta gtcgtctgcg
     1621 tttttgagag catctgcgag aggtactttc tttattcgtc ttgtgagcgc aagcgcaata
     1681 agcgcaaacg atccgacacc gatcagagcg gtttttgcgt ataccgaaat aaaggttctg
     1741 taaggcagcc agccggggta atcacgcatg aagaagacga acatctgacc gagcagatag
     1801 tctgacagcg gtatggtaac aagcagggtg acaactgtca caatggcggt tgtcgcatta
     1861 taacgccgaa aacaacaaag atattcatca tattgcccat cgaccttgta agctgacgcg
     1921 atacctttgt aagatcgtca accgttatct ctgtcgcgat ataaaggtcg tctatatcgc
     1981 ttatttccga cttggagaaa taacccgtga aattatcttt atcatacccg aacttttcat
     2041 tgaaagcgtc cttgtccata aataccgtca gcgttgacgg ataataatag accccgccca
     2101 ccctgaactt gtattccttt gagccgaact gttccctgag cgttatctca tctccgacct
     2161 tgatgccgtg tttttccgag taggcgtttg aaatataaac ctcgctgtcg tcaagtgaaa
     2221 tatcaacata cttgctgtcc ttagctatac cgtatacggt tatctcctcg cttttgagtg
     2281 taccctcgat agttttgagg gtcgttgcgg aaaacttttc agcgtccttg ttttccgttt
     2341 cgacctgagc cttcagaaga tattgatgat ccgcaagcag attggtcgtt atctcctgct
     2401 gatagctgtc gagcacagga cctaaaacaa gtccgaacat cacaagaatg tttccgagca
     2461 gtgcacctat gatcattata atatagttcg gcatattctg aaacagcaca cgcagtctga
     2521 accgcttcat aatgcctatc ttcgtgttca gcctgaacgc ctttttcttt tttctttgtg
     2581 aaaggtcatg gcggataaac tgaagaggtg acaggctgag ctttcttgtc agcattacca
     2641 agtcaattac aaatacgatt atgataggta ctatcgttgt gttgataaat gcctgcggat
     2701 tccacagcgt aacatatgtc ggcagactgt agcttccgta atatgcgctt gccgcatagt
     2761 ccttgagaag cgtatagccg agtacatttc cgacagccgc acctaagaat actaccagca
     2821 tcgggagtgc cagataatgc cttactattt cccatcttgt ataacctgtc gcgcggagcg
     2881 tacctataac gcctgcttcc tttgcaattg tgtttgaggt cgttatcgca aagatgaatg
     2941 cgatgataac cacgattata taaaggaaca tctgtacaaa ggtcgtatcg cccttaatat
     3001 catcgcccgt gaagattatt gcttgatttg tgtattccgg aatgtagccg gtgaccgcat
     3061 tcatatattt cggagcttcc tccgacagct tgtcaagaaa atcctcggac atctccttag
     3121 cctttgtgtc gtctgcggga cgctcgtcat atttccacga ataaacataa tgcagcttat
     3181 cctccggata ttctttgaat gtttccggtg tcactatcgc aacgccgaac ttgatagagt
     3241 cgaacatcat atcagacggg cttgaaaaca gcgcactgta gtccgacagt gcgaccagtc
     3301 cgcatatact gtattccttg ccgtgaagcg tcagcttgtc gccgacttta aggctgttgt
     3361 tgtctgcata cattctgtcg atggcgattt cgctgtcgtc tgacggaaat tctccgtcca
     3421 taaggcactc cagatctata tccgtacgct ttttgaatat acgcagtgtg ctgtcgactt
     3481 cctttgtcgg aaattcgaca tagaaatttt cgtatatcct tgcgtcatat ttttcgctgt
     3541 cctcaacagc ttttataagc tcatcgtcag cctttgcata aagctcaaaa ttgccgtcct
     3601 ctatattgta cttttcaaag ctttcgtcat aagcagttgc catactgccg cccgcaacac
     3661 tccagcccga tacaaacgat acggaaagca ccatgaataa aaagattaca atatatttgc
     3721 caagctcatt tttaaactcg ctcggcagac gtttaacaag aggatttctc atcgttatcc
     3781 cccttaccag tcaagatctt ctgcggcgat cttattttca ttgatataat tctttcttat
     3841 cataccgtca cgcagcttta ttaccctgtc cgccatattt ttgatagcgt cattgtgggt
     3901 aaccattacg accgtattgc cgaacctctt gttgacatcg cttataagct taagtatatc
     3961 ctttgatgtc ttatagtcaa gtgctcccgt aggctcgtcg caaagcagta tatcgggatt
     4021 tttgattatc gctctgccga tagccgttct ctgctgctga ccgcccgaca gctgattagg
     4081 cagcttgtga cggtgctcat acagaccgag cgttttcagt atctcgtccg tgttaagcgg
     4141 attatcgctt aagtatgcac cgatctcaac attttccttg atattgagat taggtatcag
     4201 gttgtactgc tggaagatat aaccgaggtg ctttctgcgg tacagtgtca gcttcttttc
     4261 gttcatatca gccgtctttt cgccgtttat tgcgatatat ccgctgtctg cactgtcaat
     4321 accgcctatg atattgagca gtgtggattt gcccgagccg gacggaccga gcagaacgca
     4381 tatctcaccc ttttcgatct ctgcgtcaat gccgttcaga acctcaacgc ggctttcgcc
     4441 ctcgccgaag tgctttctta tctctttgat tgtaagaaac attttacatt tttcctttca
     4501 tatttgctcc gctcacacag attgtacagc actgcttgat tagtaagagc taactatatt
     4561 atatattgct aacttaatta gctatagcta actcaagtgc atcatatcag tgttcttcac
     4621 gaatattacc aaaaatattt tatcacaata aagc
//
LOCUS       DAWAOG010000095         4641 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2382_length_4641_cov_2.605321, whole genome shotgun sequence.
ACCESSION   DAWAOG010000095 DAWAOG010000000
VERSION     DAWAOG010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2382_length_4641_cov_2.605321"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2078..2087
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3443..3452
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          964 a         1287 c         1033 g         1337 t
ORIGIN      
        1 cacaggcggc aagcagtgcc tttgaattaa gatagctgtc ctttgacgga gcaggtccta
       61 tgcataccgc ctcgtccgct atctgcgcgt ggagcgccgt cttgtcggct gtagaatata
      121 ccgcgacagt ctttactcca agctctctgc acgcacggat tattcttata gcgatctcgc
      181 ctctgttggc gatgagtatc ttattgaaca tacagcgttc gccttccgtt acttgatagc
      241 aaaggtgatc tctgcggata ccgctttttc accgtttact gtggcaagag ccttgccgac
      301 acctatagga ccttttacct ttattatctc gacttcgagc tttaaaaggt cgcccggtac
      361 tacctgtctt ctgaacttag cgtccttgac gccgccgaac agacctatct tacccttgtt
      421 ttcggggagt gcaagcattg ccactgcgcc tgcctgcgca agcatctcaa gcataagtac
      481 accggggact accggatatc cggggaagtg ccccttgaac cagaactcgt cctccttcat
      541 atactttgtg gcgaccacac gctgaccggg ttccatctct tcgatagtat caataagcag
      601 gaacggatct ctgtgaggaa ttatctccat tatctgctct ttattcataa gtgacatata
      661 cagccctccg ttattctatt atgatgagcg gctgaccgaa ctccacagcc tctccgtcct
      721 ttacggcgat cgactttaca gtaccggaaa tatccgcctg tatctcgttc attaccttca
      781 tactctcaac tatgcatacc gtatcgccct cgcttacctt atcgccgacc tttacaaagg
      841 gaggctttgt gggggaggga gcagagtaga acgtacccac aatagggctc tttatgctct
      901 tgcctgtatc ggcggcaggt gctgccgcgg gagctgtctg cggtgcagct gcgggagcgg
      961 ccgccatagc cataggaggc attgcgggca tcacaggagg tgcgggaggg ggatttttct
     1021 gaccaagctc tatctcaaac ttgtcgttct ttacatacat ataggacaga ccgctgtcct
     1081 tcataatacc gataaattcc tttacggcat cgctaagcga attagtttca aactgcatat
     1141 cgtgtcaacc tttctctcag tcctctacag gagcaaatgc tatacaagcg ttatgaccgc
     1201 cgaagccgag cgagttgctg agtgcacccg ttatcttcat atctctcgta ccctcaggag
     1261 catagtcgag gtcgcattcc gggtctgcca cctttaagcc taacgtgcgg tgtaccttgc
     1321 tgttctttat ctgaagagcg caggctacag cttcaacgcc gcctgccgcg cctaagaggt
     1381 gacctgtcat cgactttgtc gagtttatca ccatattctt tgcgtgttcg ccgaacacca
     1441 gctttacagc ggctgtttcg gtcttatcgt tcatcggtgt tgatgtaccg tgtgcattta
     1501 catagttgaa ggtttcgggc gctctgcctg attcctttac cgcaaactcc atagccttcg
     1561 caccgccctt gccctcgggg tcggggcttg tttcgtgata agcatcgcag gtcgcaccgt
     1621 agccgatgac ctctgcatag atgtttgcac ctcttgcctt tgcgtgttcg tattcttcaa
     1681 gaattacgat accgcttcca tcgccgagaa cgaaaccgct tctctctgcg tcaaaaggaa
     1741 tgcttgctct gtcaacatcg gtcgactttg ttatagcatg catattgttg aagcccgcat
     1801 aagcaaagcc tatcgagcac ttctccgtac cgcccgctat cgcaacgtcc atatagccgt
     1861 tctttatagc atggaatgcc tcgcctatag cgtgtgtacc gctggcgcag gcagataccg
     1921 tacagtagtt tacgcccttg aagcccgtgc ggatagcaac cgtacctgcc gccatattgc
     1981 ctatcatctt cgggatagtg aatacggaaa ctcgcttgtt gcccttgttg tgatatgcga
     2041 gaagctcctc ctgcgttgtt tccataccgc cgataccnnn nnnnnnntac ccgacccgat
     2101 tataacgccg catctgtaag gatcgagatc cttgaaatcg cttcctgcgt cctttatagc
     2161 cttatcggcc gcatacacgg catactgtgt gatgatatct gtacggcgca gttccttctt
     2221 gtcgaaaagt ccctctgcgc tgaagtcctt tacagaagcc tttacattaa gctcttcgct
     2281 ctgaccggga aaccactgct cgagtgtgaa gccgtgcttg ccctccataa gactgttcca
     2341 gaattcttcc ttggtgtttc ctatagggct tactacaccc acaccggtaa ttacaactct
     2401 gctcattttt tgtatttcct ttcgtggctt gtctttacat tgataagccg ccgcttatct
     2461 ctattacctg acctgttaca tagcttgcgt cctttgaagc gaggaatgat acaacgcctg
     2521 ctatctcctc gacctcaccg catctgccca tagctatctg ctttaccatt gccgctctca
     2581 gctcctccga aagagcgtct gtcatagccg tgtggataaa gccgggcgct actgcgttac
     2641 aggtgatgtt tctcggaccg agctccttcg cggtggtctt tgtcataccg attatagccg
     2701 cctttgtagc ggagtagttc atctgaccgg ggttgccgta aagacctgcc acgcttgcaa
     2761 gatttataat tctgccgctg cgctgtttca tcataacaga gcctgcaagc tttgtcatat
     2821 tgaatacgct tttctggttt accgcaacaa cgaggtcgta gttttcttca ctcattctta
     2881 caagcagacc gtcacgggtt atacctgcat tgttgacaag cacgtcaata ctgccgaaat
     2941 cggcctttac gctctttaca aaagcctcac actgatcgta ctttgatacg tctgcgatgt
     3001 acttttcaca ctttacgcct tctgctttta tcttgtcgat tatgtcgttg ttttcaaagc
     3061 agctctcggc gatgtcattg agcgctatgt catatccgtc ctttgcaaga cgcagagcta
     3121 ttgccgcacc tattcctctt gcagatcctg ttatgatagc agttgccatt tattttctcc
     3181 tttgttttat cttacttgtt cagcagtttt tcagcctctg cgaacatttc ttccactatt
     3241 tcctttgcgg gctgtacctt ctttaccata cccgcgatag ctccgcataa gaacgagccc
     3301 tcctcaaggt tgccgtcctg taccgcctta cgcagtgagc cgagcgttat ctgctcaaac
     3361 tcatcgggag tgatagttcc gttcatttca ccgcttgcga gcttcttcga gaacttcgtc
     3421 tttacattgc ggcaggggtg acnnnnnnnn nnccctcagc accgagcata aagcttgcgg
     3481 caattcctct gccgtctgcg ataccgcctg cggcgataac agggatagat acagcgtcaa
     3541 ctacctgcgg aacaaggcac attgtggtgt tttcaccgat gtgaccgccc gattctgttc
     3601 cctctgcgat tactgcggac gcaccgcttc tttccattct ctttgcaagc gccacactgg
     3661 gtactaccgg aatgaccttt atgcctgcat tgttccacgc ctccataaac ttgccggggt
     3721 tgcccgcacc ggtcgttatc atcggaacct tttcgtctat aacgagctgt gcgagatcct
     3781 cagcattggg gctcataagc ataatgttca cgccgaaagg cttatctgtc agcgactttg
     3841 ctcttcttat ctgatctctc agataatcga taggagcaga accgcccgcg attatgccca
     3901 cgccgccggc atttgataca gccgccgcaa gtgtgctttc ggcgatccac gccataccgc
     3961 cctgtaatat ggggtacttt ataccgagta attttgtgat ccttgtttcc attgttttta
     4021 tcctttcagc tgtggattag tattcaaaaa cagttgcgcc gtaggtaagt cccgcaccga
     4081 agccgacaag acatatcttc atgccgcgct tcagtctgcc ctgacggtca agctccgcaa
     4141 ggcacatagg gatacacgag cttgatgtgt tgccgtgtgt ttcgaggttt gtgaacacct
     4201 tgtcaagatc cacgttcatc ttcttgagcg cggactggat tattcttata ttcgcctgat
     4261 gggggataac aagatctata tcgtcaagcg atattccgga atgtccgcat acatattcga
     4321 ctgcattcgc catagcgtca acggcgaact tgtatactgc ctttccgtcc atctgtaaat
     4381 agcgctcctt gttcggagtg tctatgatat ccttaaggca atcgtcaccc ttgaaatagg
     4441 ggttgttcct gtcgtagaaa ttcttgcagt aaaggctctg gagcatatcg ccctcagcgc
     4501 cggagtggct gtaatattcc ttgccctctc ccttttcgat aactaccgca cccgcgccgt
     4561 cgccgaaaag tatacagctt gctctgtcgg tgtagtctat ctggcttgta agtcgctcac
     4621 ttgctacaac gagtatagtc t
//
LOCUS       DAWAOG010000096         4637 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2388_length_4637_cov_2.618725, whole genome shotgun sequence.
ACCESSION   DAWAOG010000096 DAWAOG010000000
VERSION     DAWAOG010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2388_length_4637_cov_2.618725"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    460..469
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1527..1536
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3595..3604
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1032 a         1196 c          978 g         1401 t
ORIGIN      
        1 gatacaaggt taagacacac agggcctaaa atgataccgg caaccagtgc gccgactacc
       61 tgaggcagct ttataacctt tgacagcagt ccgaatgctt ttgtgaaaat gaggattatc
      121 gctatataaa gcagatatgt gtaatccata attcatcttc ctttctgttt gttctacttt
      181 ttcttttcgt cacaaaggct atatcgctgc tgtgcgatat agcctctgtt ttttctgtat
      241 gcccgtatta taacacctat ttagaacatg tcaatctatc ctcgcgattt tgcagagaaa
      301 aaaatcataa aatctatgta ttaaccaaag cgaactattt taagtggaca aatttattga
      361 ttattttcta tgcaattttt taatggctcg ccattcattt tggcgatact tctttttgca
      421 aagatggcat aaaaagcata tctccctact gctcacattn nnnnnnnnna gatccgcata
      481 aataaaagcg ttacgggatt tttacattcc cgtaacgctt ttatgattat gaatttctca
      541 taaatattta ttcagtatcg gaacatttca gccgaccttt tcaaccatac atacagcgtg
      601 tgcggctata ccctttcctg ctatgccaag accctcctcg gtagtcgcct tgacgttcac
      661 atcgtccgta tcaagcccca gcgccgccgc tatgctctcc tgcatatccg gtatgaagct
      721 tgcaagcttc ggcttttcgg cgattatagt tatatccgca tttgtaacgt gtgcattttt
      781 ctcatcaagc agtttcctga cgtgttcaag aagtcgcata ctgcttatac ccttgtatct
      841 gtcatcgctg tcgggaaaat gctttccgat atctccgaga gccacagctc cgagcatagc
      901 gtccataagc gcgtgtaccg caacgtccgc atcgctgtgt ccgagaagtc ctatattatc
      961 cttattcggc acttccacgc cgcaaagtat cagccttctg ccttctacaa gcctgtgcac
     1021 gtcatatccg tgacctacac gaagctttgg tgcgcctgtt ttaccgataa catattctgc
     1081 cattgatata tcctctttcg tggtcagctt tatattgtcg tagcttccct caacgataaa
     1141 cggctttatg ccgtacagct ccatgaccga tgcatcgtcc gttatccttg cgtcagtgac
     1201 atcaagcttt tccgccgccg cgagataatc tgcctttctt gccgcctgtg gtgtctgagc
     1261 aagccatagc gcgcttctgt cgggggtggc ggtcacctcg ccgctaccac acaccttgat
     1321 cgtatccttt gctcttaccg ccgccaacgc cgcaccatgc tcatatgccg ccagtgctac
     1381 attgcagcca agctccgcag tcaaaaaagg tcttgctccg tcatgtatcg acacaacatt
     1441 gctcttttca gaggtctgtt ttacagcttt cagtgcggac tcaaagcgtg ttgagccgcc
     1501 cgtgatgatc tttatctgtt tgtcggnnnn nnnnnntgca catttttgct tatcagctct
     1561 ttgattttat tgatattatc ctcgcttgcc gctattatta tctcatcggc gttgctttcg
     1621 ataaaccgct ccacactgta cagtatgacc gccttgccgt gaagcaatgc cgtcatcttg
     1681 tcaaagccca ttctcctgcc gcttcctgcg gcaaggatta tcgcacttgt aaacatacct
     1741 ctcctttacg atcagtcaaa atgcttgtag ttgccgagaa atttaaagta cttcatctct
     1801 tcctcaagta tagtcataag ccttaccacc tcgggatcaa gcgcatttcc ctcaaagtcg
     1861 aggtagaata caacgtcaaa cgtttcccgc tttatatcct gctttgcctg aggcacaggc
     1921 gcactctcta tctttgtgat attcagtccg tacagtgcaa aacggataag cattctgtac
     1981 agtgagcccg tggtatgcgg cagtgacata catatcgaga ttatatccgc gtcgggagtg
     2041 acctccgggc gctttgatat gcagataaag cgcgttgtat tgtccgggtt gtccgcaatg
     2101 ccgcgcttta ctatctcaag accgtacagc tttgcgcatc tctccgagca tatcgccgct
     2161 accgtattat cgtcactgtt tgcgaccaat tctgcggcaa gtgccgtatt tgtatatggc
     2221 acttgtcttg cggagctttc ctttaaaaat ccgaagcact gctttaaagc ctgttcatgc
     2281 gaatatatag tctttatatc cgctccgggc tttgccgcaa ggcagtgatc tatctttatc
     2341 tgagtgcgct tgcagatata gaaattgtac tttgcgagca ggtcgtaggt ctgccttata
     2401 tcgcccgccg tgctgttttc gatcggaaga acgccgtaat ccgcactacc gctttcaact
     2461 gcctcaaaca catcggaaaa gtcgggatag aagtctatct ccgactcggg gaacagtttt
     2521 accgaagccg cttcggtatt actgcccgca gtacccgggc aggcgatcct tatggattct
     2581 gttttcgggc ttgcaaactc aggtgtcggc gcgtcagcaa gtatcctctg ctgtaagcac
     2641 ttggatatat ccataataca ggtgaacaat tgtcccgcac cctcggaaat atcctcgggt
     2701 gcttcgctct ttatacgctc gagtatttcc tgctcgcgct ttccctgaaa cacctgcata
     2761 ttgttttctt tcttgtactt tgcgaccccg acacacagat ccatacgctt cagaaacaat
     2821 ctcagtatgt catcgtctgt cgcgtcgatc tctctcctta tctctttgag atccattttt
     2881 actctccgtt tctttatttc ggtattttat acattataca gcaattgccg cagaactttt
     2941 ttcaaagcac tgcgacaatg cataattttc agtttcagtc tgctttcgat tccgccgagc
     3001 tttctgccgc agctgaagaa gatacggagg acgttttgga agacgcatcg ctgtttaccg
     3061 ttgccttggc atagtacaca gttaccgttt cggaatttgc aacatcgacc ttttcgccga
     3121 cctttttact gcatttaacg acatatcccg cctttacgcc gttcgtgtcg ccggtttcct
     3181 tcttttcaaa cttaacgttt ctctgcgaca gcatagccgt gtactcatca accttaagac
     3241 cggtgtaatc gggaagcgct atagatgaag gtcccttgct gactacaagc tttatctcag
     3301 tgccttctgt gatctcggta tcgggcttga tgctctggct tattactatg ccgtttgcga
     3361 ctgtatcgtc gaacttatat tcggcgttta tctttacaaa gttgtatgtg tcctgtatag
     3421 acgtaaacag cgcgcctgta aatgtcggac agccgtacac gatatccggc attgtatcgg
     3481 aggataccgt tgttgtagcg gcggtagttt ccggagcgct tgaagatgcc gtgctctcgg
     3541 gtgcggaata atagttcggg tcgtatgacg agcttgtatt attatctgtc attgnnnnnn
     3601 nnnnaagcac cgcgatagcg atgccgaagc cgatgattat aacgccgcac acgataatag
     3661 ttgcgataat cgccgcttta ttattctgct ttttcttctt gcccttagcg tttgtgcttt
     3721 tctgcacagg cgctttttca ggctcttccg tgacctcgat acgctttaca cgctgaaccg
     3781 gacgaaccgc ctcctgctcg gcggtattga acttaggctg ctcaaacagc tttacaacaa
     3841 gctctgttat cgtctgtata cggtttgaag tgttcagctt cagaccattc attataacct
     3901 ttgaaacgtg gctgggcaca tttctgttta tcaccatagg ctcggggcag ttgtcattgc
     3961 caagtcttga aatagattcc gcgggcacac agccggtcaa caccctgtac agagtagcgg
     4021 atatgccgta tacatctgtc cacgtaccct gccactctgc ggacgaatac tgctcgggag
     4081 cagaatagcc ggggtacatc tcgcacgcaa tttccgtgcc tgtcgttctc cctgcgctta
     4141 tctgaaaatc tatgagctta agctcgttct tctctgtcac aagtatagtc tgcggactta
     4201 tcccgcggtg gattatccct gcggagtgca caaggctgag cgtggtcagt atcgggggga
     4261 agagctcctt taccctgtcc catgtaagct cgcccgagca gttggcaaga taatttttca
     4321 gcgttatgcc gttaataaac tcgaatatta catacgccgt attgttctgc gggaaaatat
     4381 caagcaccgt ctgtatgtgc gtcattgaac gtgccttgag caacgctttg ttaagctcca
     4441 cgaactccga catatacgtc ttgtaaagcg gaagctggtt agggtcaact attatctgcg
     4501 gatcgccctt cttgcgtgag cacagtgtat cgggcatata ttccttgatc gtgacctttg
     4561 taccggtcac cttgtcaaaa ccgatatatg tagctccctc gccgttgtag ctgagaagct
     4621 ttcctgcaat ataacgg
//
LOCUS       DAWAOG010000097         4616 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2405_length_4616_cov_2.058978, whole genome shotgun sequence.
ACCESSION   DAWAOG010000097 DAWAOG010000000
VERSION     DAWAOG010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2405_length_4616_cov_2.058978"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1474..1483
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3602..3611
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1113 a         1086 c          976 g         1421 t
ORIGIN      
        1 aacagctttg cgtctatgct ccagtactcc tgagccttga agttctcaat ttcctgctct
       61 ctgtcaacga taagtcttac agcgaccgac tgtacacggc ctgccgaaag acctcgtctg
      121 acttttttcc ataagaacgg gcttaactta tagcctacta ttctgtcgag gatacgtctt
      181 gcctgctgag cgttgataag atccatatca agcgttctcg gatggctcat tcctgcctca
      241 ataccgctct ttgtaatttc actgaatgta acacgcacgg gtgcatcggg gcttatcccg
      301 aggacgtgcg ccagatgcca cgatatagcc tctccctctc tatcagggtc ggtcgcgagg
      361 tatactatat cggatgcctt tgcgtctttc ttaagctctt ttataagagc cgccttatct
      421 tttttgtttt cgtatttcgg ctcaaagtta ttatcaacat caactccgag cttcgacttc
      481 ggcaggtcgc gtatatgacc tacagatgct actacggagt aatcctttcc gagatacttt
      541 tcaattgttt tcgcctttgc cggcgactca actattacca gatgggacaa attttcgtct
      601 tcctttccgt gccgatgcgg caccccaaat tcgcctttca aactgtttgt gcgttgctgc
      661 agtttgtgta cggcgagcgg ctgcgccgcc ccctcttaac gtttaatatt tgcttttctt
      721 atcgtcaagg tgttgttttt attaaacagt aagcatatcg tctgctgttt acgcaagcct
      781 cttaacgttt tatttcaatt atttgaatta ctgtctttgt gctgttgaga atttaacatt
      841 attccggcga gcatagcacc ggataaaatt gccgcgatct caacgcgtaa ttatatagtg
      901 cgattattaa ccgagtttaa catcaaacta ctttctactc tttgacaatt cccatgcaat
      961 gaccgatgcc gccaccgatg cgttaaggct ctcggcactg cttatcggaa tgaacagcga
     1021 cttgtcgcaa aggttcttca ccgtatcggt aacgccgttg ccctcgttgc cgatcacaac
     1081 agctgtcttt tcggggagct tttcctcgcc gagcttcacc gacctgtcgg aaagttctgc
     1141 cgaatacacg gtaaatcctc ccttttgcaa aagcgtgatc gcttcttcgg tttcacagca
     1201 gtatatcgga aggcggaaca gcgaacccat cgcgctcctt acggtttttg gtgcaaacac
     1261 gtccgcacaa tctccgacaa gtataacacc gtcgatcgag aatgcgtcac aggaacgaat
     1321 aatcgttcca atgttaccca tatcttgtaa attttccaat attataaact gtctgctact
     1381 atctattgta cttaaattca gaattttgtc aagatgtctt actgtaataa aaattccctg
     1441 gggggatttt gtgtcggaaa tactgtccgc tacnnnnnnn nnnatctgta tcattttgca
     1501 gtgctcctgc aaaaggctga tgcattcggg atatttttcc gcggcggcgg cagtatagta
     1561 tgcgttttcg cctaaggcgc acccgctctt aactgcttca acaacaagct ttgcgccctc
     1621 gcacgggaaa aatcccgact gtctgcggta ctttctctgc tcgaccagct ttcggtattc
     1681 ctttatataa ctgttttgct tagatgctat ttccatattt ctcattaaca ttcagtttct
     1741 tgacttttac ctgttgttat gctaaaattt ctttatccgt gtacggtaaa gaaaggactt
     1801 attacatgat tttttccgaa ctcagcctta ttgaaaagga aacagggctt aaatacgacc
     1861 cggaaaccgc tacgctcagc ggcgtgaaga acggcattcc tatcgtggtt ttcgacaacg
     1921 acaaaaagca cagatttgac atagtgtgcg gtgcggtgat aaccgaccga cagttcggca
     1981 ggatatacac tatggctgag cgctttccca agaaaacaat gctcggtgtt gaaaacgttg
     2041 acggctgcgt caacatttac tgcaagggat acaatctgat gcaggaaaat ctaccgttgc
     2101 tgattgaatt tttggaaaaa ctcactgcct atttaaaaag cgaaatgcga agtacaacgc
     2161 ctattcaggt tgcaaaggtg ctggagcttg aagattacga gatactgaca aggcgcagac
     2221 gctccgacgg aactgtccgc ccgccgtttg acgtaaaagg cactgtaaaa ggtgtgctcg
     2281 gcggtattct cggacttgtc atcggctctg ctattttcgt ggtgtttata atgatgtctg
     2341 accttgtcgg ctggataggc ggcgttataa tgtctgccgc ggtaatatcg ctctatactg
     2401 tgttctcaaa gaagctgagg actatcgact gtatattaac cgcaatactt acatttgcgg
     2461 gttgggtatt ttcaaacggc tttgcttatc tgttccagat attcttaaag gaacaggaag
     2521 cgggcactgt tgtcaacata ttcggcatac ttgataatct ggcaaaatac tccatgcagt
     2581 ataccgagct gtcgggcaat atgctcaacc gtcttatcgt gacatttatt ttcgttgttg
     2641 cgggcgctat cggaagctat atattctact acaagcacca cagcaaggat atgtattaaa
     2701 aggatttata tacccggcag accgccgggt atattttatg ccttttaagc catcggctct
     2761 gcaccttaaa tataaaaaat cacacgccca taataatgtg ggcgtgttgt tttctcttaa
     2821 agagcagcct tagccttctc aacgagtgca gtaaagcctg cggggtcgtt gatagcgatc
     2881 tctgacagca tctttctgtt aagaccgata ttagccttct ttaaaccgtt gatgaatgtt
     2941 gaatagttag taccgttcat cttgcaagcg gcatttattc ttgcgatcca gagctttctg
     3001 aaatctctct tctttaaacg tctgctgatg taagcgtact gacctgactt ccatacagct
     3061 tccttagcgg tcttgaagag tctgctcttg ccgccccagt agccctttgc aagctttaat
     3121 gttttatttc ttctcttacg agttgctaat gcacctttaa tacgagccat cgttaaattc
     3181 ctcctatagt gaatattgcg ccgaaacggt cgcagttagt ttttatttgt aaggtatgag
     3241 cttcttaact tctgcaacgt tcgtcttgtc gcagtatcct gctgcacggt tgatacgctt
     3301 tctctttgta gccttctgtg tcagtctgtg acgacggttt gtcttctggt acttgacctt
     3361 accgttcttg gagaggttaa aacgcttttt agcaccgctg tgggttttga tcttgatctt
     3421 ctttgccatg agtggaatcc tccttattta ttttccgccg gtttatcggc gtccttcttt
     3481 gctttcttac tgtttgccgc tgtcttggga gcaacaactg ccatatagtt cctaccctca
     3541 agcttagccg gcttttcgca tgagccgtac tctgaaacag catcaagaac tctctgcata
     3601 annnnnnnnn ncgaaccttg tcgccgttct taaggaactt tattgcctga ttagcctttg
     3661 tgttaaagtc gttggtgtcg atattgagcg acatctgtat ctctttaagt tcagcctgtt
     3721 tctggttctt tttagattcc ttttccttct ttgactgctc aaaacggaac ttgccgtaat
     3781 ccataatgcg gcataccgga ggcgtagccg aaggagcgat aagcacgagg tctaagtcag
     3841 cctcgatcgc catatcaaga gcctgcgccg aagacataat gccgagctgt tcgttatcag
     3901 ctccgatcac gcggacctct ttttcattta tctcttcgtt gatgagttgt tcttttgtgc
     3961 tgattgtaaa gcaccgcctt tctgaaaaca aatcgtttta tgcaaaacaa aaagcgtgat
     4021 aaactgcaat gcagattcac tcacgctgta caaatattac agacggaact atgctgtagc
     4081 ccgtcgggaa tttctgtaat tacctcattg aacagcctta cggcgtaaat gggtgagagt
     4141 gcttctctgc ttttgtttta caatttaatt tacttgaaga ttatacaaca ttaaagcggc
     4201 gttgtcaata gggaaagtga aattttttcg gaattttgct tctttactgc aacattcctg
     4261 cccgatgtac cgaaatatgc aactcagaat gcggtcgaat attccgagaa cggcggcaag
     4321 gcactcatca acaactttac aaatgctttc ggcgagagct ttgacaagtc ggtacacgaa
     4381 atctcaaacg ttatcggtga gggaaagatc gctatcggcg gcattgactt tgtgataacg
     4441 aaaactgccg aagcgtttga cattgagata cccgagatca acgcggttta tactcatatg
     4501 ctcggtcacg attgtcactc catcgtagca ggtgccggtc atgcagacgc tatcatcggt
     4561 cagctgaacg gctatatcaa aaagggatac acactgatac ttacatcgca ctacac
//
LOCUS       DAWAOG010000098         4613 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2406_length_4613_cov_3.049144, whole genome shotgun sequence.
ACCESSION   DAWAOG010000098 DAWAOG010000000
VERSION     DAWAOG010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2406_length_4613_cov_3.049144"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    582..591
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3362..3371
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1019 a         1136 c          941 g         1497 t
ORIGIN      
        1 gccgtaagcg ggacgctgac gctctgttcg acccggggga ctgtacagat atcttaggat
       61 agtgcgtttt tggaagtttc ggtgacagtg cagttgcatt gttaagctca caaaattatt
      121 aaaatcttgt cggcacggaa gctgttttca gcggtgttgg cgatatattt attgctttgg
      181 cattttttta atatgttgca ctgcaatttc tatgaaactt gataagcaga gaaattgtgt
      241 cacagttcct tctttttacc ctcatataag aaaacagctg tatggttttt agccatacag
      301 ctgtttttgg ttattcacct attattcttc ggtttcgtcc tttttcttac gcttgccgcc
      361 gaggagaagt cctgccgccg ccaaagcagc tgcagcagat gcccctgcaa caaatgttcc
      421 gttaatacct acaccggtat taggattatc gctactgtca aagtcaacgc ttgttcgatc
      481 accctgaggg gttctatcgt caaagtcgac ctcatcaccg tttacacgat caccttgagg
      541 tgtacgatca tcaaaatcaa cctcgtcgcc gtttacacga tnnnnnnnnn ntgttggcga
      601 tatattacat tatatcagct tgcaaacaat gtaaattcca atgagtattt tcacagaaag
      661 aaccgcacgg aaataccgtg cggttcttaa catataagct tttgaccctt atcaatactc
      721 cgacagctca taattatccg gcgttctgtg ccagagcccg tctgtctgct cgtatatctc
      781 cttgaacgtg aacttgcgga agtttatctt acatctgccg ccgtagttcg cctcatatac
      841 ccatataccg tccgtatcga cagagtatat cgtgacatag tgcggatatc cgtacattat
      901 gttatcatat gccagatagt cgccggggcg gaacgtatcc gtcatccact ttttcagtgc
      961 cgcttccgtc cagtttccgt ccgagcggtt ctttatcgta agagtatttt ctttatagcc
     1021 gttcgtcttg ccgaacagat attcaaacac ttctatcgta aatcccgcac actgtatgcc
     1081 ctgatttccg agcacattac ttcttccgat aagacaggta cagcccgttt catttcctgt
     1141 ttcacagaag ctgtggcagg tacagggcga agtcagcagg gtattataat atttgccgag
     1201 ctgatagctg tgccaaaagt cgtttatctt cctgtcccac ttgccttcca tcatctcgtc
     1261 aagacgcttt tgcacaagtg cttccatgct gttcttttta actattctga acgtgcccgt
     1321 tactgttccg gtgaagttcc ctttacctgt aacggtaaag gaagcagtgc cggcattaac
     1381 attgttctta tagcccgaaa cagtgtagtc ggtgtcctta gcaagcgttt ttccgttata
     1441 tgtaacagtc ggaacaggag taagcgtatt gcttgtatac tgctgatctg cgcagattat
     1501 ccttgcagaa gatatagcgg caggcagtac cttgaacgaa gccttgatct ctgcaccttc
     1561 aaagcttcct atgcctgtta tcactgcggt tgccgttccg gcatttatat tgttgtatgc
     1621 actgaggata tagtccgttc ccgctttgag tgcggaagaa cctatcgtta cagtagttgc
     1681 ggggagctga gcaccgccgc tgtatgttac atcatctaca cttaccgttg cattttcaaa
     1741 caattcaagc gcaccgctga tctcggttat tttcagtgtt ccgttataat tgcctgcgcc
     1801 tgtcactact gccgaaacat tgcctgccga ctcatcgata tcgaccttat aatctgtatc
     1861 ctgcgcaagc agtataccgt catattttac ggttaccgta tagcccttgc cgctcgttga
     1921 aagcgataat gttgcctttg ccgcagatat gtcagccttt tcgaccacgc agtcggttgt
     1981 aacactgcct gtaaagttac ctgcaccgtt tacagcgacc ttatgttcac cggggagcag
     2041 gtcggtatcg gttacttcgc tgtcacccgt cttaagctca tactcaccgt actttccgct
     2101 gacagtaaat attgtacctg caggcgaata tacacctttc acattaagct gagcatcagc
     2161 aatgtcagct ttggaaatga tgaacgcctt agttatagca ccctcgaact ttcccttacc
     2221 cgtcagcgtc agctgtgcgt tacctgcatt tatattgttc agatacgaaa cagtataatc
     2281 agttccttct ataagccgca ggctgcctgt ttttaccgtc aatgccggct ttacttcctg
     2341 tcctgtaaat atcgcattcc cgataactgt atttgctttc ctgatatcag cctttgatat
     2401 cttgaatgtc acggttttac tgccgctcag atgatttttg cctgttacgg taacacttgc
     2461 cgtgccgacc tccgtgttat ttttatagga aagggtatag tccgtaccgt ttttaaactt
     2521 ataagtgccg tagcttaccg tcaccgcagg cttcacctgt gccccggtgt agcttgctcc
     2581 cgcagctgtt atttttacgc cgcttacagc tcttgcggta attttaaatc tttttgttac
     2641 agtgcccgaa taattgccct tgccggtaag cacaaccgtt gccgtaccaa gtgcggtatt
     2701 attcttatag ctcaatgtaa aatccgttcc gtttttaagc gtttttgctc cgatctttac
     2761 aacaggcacc ggctttatcg ccgccccggt aaagcttttt acacttatat ttgaaacagc
     2821 cgaggcaggt atcgcttttc ttgttatcct gaaagtcttt tttacgcttc cgccgtagat
     2881 acctttgcct ttgacggtta ttgtagccgt tcccgcattt ttgttatttg aatacgatac
     2941 agtgtagtcc gtattcttct taagcgttgc ttttccgtac ttcactgtaa ctgcgggact
     3001 cagcgctgta ccgttatatg tcagagaggt cttatatgcg atcgttgctt tcgatatcgg
     3061 tttggggttt atcttaaacg taactctctt tgacccggtc agaagctttc ctttgcctgt
     3121 aacaactact gtcgccgttc cgactttagt gttattcttg tatgtaagcg tatagtctgt
     3181 accgttctta tatgtcttgt tgccgtattt tacggtaacg gcaggcttca atgcctttcc
     3241 tgtgtaagtc accgccggga cgcttatatt cagcatagaa accggtcttg cgactatctt
     3301 gaaattcaga tagcagctgc ctttatagtt gcccttaccc ttgatattta ccgttgccgt
     3361 tnnnnnnnnn ncattttttt cataagtcag agtaaaatcg accccgtttt tcagaactgt
     3421 cgagccgttc ttgataagcg gtaccggttt cagctgtgat ccggtatagt actgcgttgc
     3481 taccttatag aacgaacacc tgttatatat gaccgcaggc tggattttga acgatttttt
     3541 aatcgttccg ctgtaacttc ccttgccctt gattataaca tacgccgttc ccgcattttt
     3601 attattttga taacttacgg tgtagtcgat gccgtttctg agcaccttgt tcttatactt
     3661 tactgtcggg ttctgcttta ccgcacttcc tgtataagtc tttgtcgtga tccccgaaac
     3721 cgtacatgac gatacagccg ccgcatcggc gtttgttccg aaaaaacagc aaacggcaac
     3781 catacaaagc aatccgcata taaacgccgt gatctgttta aaaatcgtca tatacctgct
     3841 tttaaccgtt gccgtcatag aatatgcctt tccctatgcc tgccgcctta tttgaattcg
     3901 atatcgttcg gtccgcagac tatgttgcac ttgccgtact ccgccgatgt gatagttgta
     3961 aagctgttca gatttatcct tacattggga taaacgcact tcgtcaccct taccgacagc
     4021 gtttcctttg cgttgattat tctttgtatt tcttctattc ttgctctcag cggctttttg
     4081 tccataccct tctggacctt aagccttatg gcagaagaaa tgtaagcttc tttttctgcg
     4141 tcaagctttt cgccgttacg cttttttcct tgtaaaaatt ctatggatgt gttgatcttt
     4201 tcgatatatt catcgatagc ggtacactgc ttttccagcg agtccatttc aagcaggagg
     4261 ttcgtggtac ttcccacaga tactacagtg ggcgtatagc tcttgtgtcc gagctgtgcc
     4321 accgcaaatt tgcccataac actgcaatcg ccgccgacaa gtgcgccggg gctcttggtg
     4381 cagtcaaggt cgccctctat ctttatgcga cagccgacaa gcgatgtgct tgtaagatta
     4441 cctctggtcg ttatggtatc gttttctccg attgcaatat ttatattacc gtattgcgac
     4501 tgcacagtac ttgcaaatac accgttctta acggttatgt cgccctgagc ggtcaatgtc
     4561 gcacctgtta ccatacccaa tactgttatg gattttcccg ctgtgacagt ata
//
LOCUS       DAWAOG010000099         4605 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2413_length_4605_cov_2.326593, whole genome shotgun sequence.
ACCESSION   DAWAOG010000099 DAWAOG010000000
VERSION     DAWAOG010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2413_length_4605_cov_2.326593"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1155 a         1042 c         1120 g         1288 t
ORIGIN      
        1 gcctgtggag aatgctccaa gagcgccgta tgtacaggtg tggctgtctg cgcctatgca
       61 cagctcaccg ggacctataa gacctttttc ggggagaagt gcgtgttcaa tacccatctc
      121 gccgacatcg taataatttg taatatcata ctttcctgcg aactctctgc actgcttgca
      181 ctgagtagcc gccttgatat ccttgttggg tgtgaagtga tccatcacaa gcgaaatctt
      241 atccttgttg aatatgccgc cgaagcctgc cttgttgaac tcgtttatcg caacgggact
      301 tgtaatatca ttaccgagta ccatatccag ctttgctctt ataagctgtc cctctcttac
      361 gctttcaagt cctgcgtgct ttgccagtat cttctggctc atagtcatgc ccatgtgttg
      421 ttcctgcctt tcattatgcc ctttataggg gctgaatatc tcataattat ttccgtttat
      481 cagtcacgtt caagagctga gatacctgtt cttacaagct cctttactcc atagggctgt
      541 aacagtccta tgaacgaatc tatcttttca ggctcgcccg taagctcgag cataagtctg
      601 ctttccgcta catttacgac ctttgctctg aaaaggtttg ctatctctat caccggctga
      661 cgcgatgcag acccgttgcc gaccttcata atgatatgct cgcggcatac ggcatcatga
      721 agtaccttta cctccgatac gtcatagagc ttgcggagct gttttaccag ctgttccatc
      781 acaccctcgt cgccgtctgc gactatcgtc atacgcgaaa tgcctaggtt ttcggtttcc
      841 gcaacgttaa gacttattat attatatcct ctgcggctga acagaccggt tactctgccg
      901 agtacgccga tgccgttgtt tacgcagacg gaaattacgt ttctctctgt catctttctg
      961 cctccgttca gtcgtcgatc tctatctcaa ggataggctc ggttatggat tttcctgcgg
     1021 gcaccatagg aagaacgttt atatccttgt ctattctcgc ctcgataagc acgggagctg
     1081 tctttgaaac ctcaagagct tcacgcagta tttcctcgca cttgtcctcg ctgtcaataa
     1141 cgaatgactt tatgccgaat gcctcgccga gcttcatata atctgtagga cggtcaagtg
     1201 ttgtctgtga aaatctcttt ccgtagaata atctctgcca ctgacgtacc ataccgagaa
     1261 cattgttgtt gataacaagc tcaacaacgg gaatgttgtg ctttgacagc gttacaagct
     1321 cgtttaagtt catatggaag ctgccgtcac ccgcaatgtt tacgaccgtc ttgtcggggt
     1381 ttccgcactt tgcaccgata gccgcaccaa gtccgtagcc catagtgccg agtccgccgc
     1441 ttgtgatgag ctggtggggg tgcttgtatg cgtaatactg agccgcccac atctgcgact
     1501 gacctacctc ggtcgcgata agcgcctcgc cgtttgtcag cttgtcaagc gtttcgataa
     1561 ggtgctgagg agtaagagta agttcgtctg tgcctttctg cctgcactcg tactgcttct
     1621 cccacgtctt tatcttgccc atccagtcgg tgtgcgactg ctgaggtata agctttatca
     1681 gctttttcat aacgtctgtg ctgtcgccga taacctgatg ataaaccttt atgttcttgt
     1741 ttatttcggc agggtcaatg tctatatgaa gtacctttgc atcgggggcg aaggtctgcg
     1801 gattgcctat tactctgtcg gaaaaccttg tgccgatacc tatgaacagg tcgcagttct
     1861 ttattgcctc tgccgctgtc tttgttccgt gcataccgag catacccata tatctgctgt
     1921 cggtttcatc aaagctgccc tgacccataa gcgaggatga tacgggagcg tcaataagct
     1981 cggcaaaagc ctttaatgtc tttgctccgt cctcgccgta gataccgccg cctgtataaa
     2041 ggaacggacg ttcagcgttc tttatcagct ccgccgcctt cgctatacag ccgtcctggt
     2101 gcatagtcac attcaccgtg tacggcttta agggcttgtt tacaaactca gccttctggg
     2161 ctgtaatatc cttaggaaca tcgacaagga caggaccctt tcttccgctt cctgctatgt
     2221 agaatgcttc acgcagtgtg tcggcaagct tgtttacgtc ctttacgatg aagttatgct
     2281 ttgttatcgg cattgtgatg cctgttatat cgacttcctg aaagctgtca agacctaaga
     2341 gagagcaggc aacgttaccg gtaatggcta ccataggtac gctgtccata taagccgttg
     2401 cgatacctgt tacaaggttt gtcgcaccgg gtcccgacgt tgctataaca acgcccgtcc
     2461 tgcctgtcga acgtgcgtag ccgtcagccg catggcttgc gccctgctcg tgtgctgtca
     2521 gaacgtggtg tattttgtcg ctgtaattgt ataagctgtc gtagatgttc agaactgcgc
     2581 cgccgggata accgaaaacg gtgtcaacgc cctgctctat cagacattcg cagattatat
     2641 ctgcacccgt cagatttctc atttctttca ttcctttttc tttatacaga ttatttatag
     2701 taacggataa aaatcctatc gttttatttt aacactatac agcgtttttg tcaatggtga
     2761 aaccgcattg caataaagcg catcgtacaa atttgcggtt ttttgttttc cggtacaagg
     2821 gatcggacgg tcgaacaaat gagcgttttg cggcaggata aaaacaggaa caggtactgc
     2881 tgtgggaata gttacaaaac ggtgacaaat ggttacaaaa tagttacagt ttcgggtttc
     2941 ctattgtgaa gaagttttgt ttgtgataga atatttgtat atgatattgg gaaagcggga
     3001 aatttacggg aggaggaaaa tatgatctat accgtgaaaa tcgaacagac aaatgcttat
     3061 ccaaagcgta tgaaatacgt ttctaaaaca gattctttca tcgaaaagga ctgcgacagc
     3121 ctttcgtatg tgtgtaatgt acttcagctt taaggaaaag cgatcgcacg atatgtaaat
     3181 caaagagtgt gctgagatta taaattaagg caagatatga tataatggag aaaatatatg
     3241 agcaaaatgt gcccaaaatg taaacgtgaa atagacgaga acgtgaaatt ctgtccgttc
     3301 tgtgcggcgt ttaccggaga aacaccgcag gaaaagaaga aaagccctgt cggtacgata
     3361 ataggcggag ctgtgggcgt gttggcactg ggtgcggcaa gccttgcggt gtttgtgttt
     3421 gacgtgttcg gaatgtatag tgacaaggca gaaacttttt acggcacagg cgacaagctg
     3481 ttcatacagt cagtgacccc tgtttgcatt gacggaaaat gggcatatgc cgacaaggac
     3541 ggtaatacca cactgaagcc gcaatataca gccgcctatg ccttcaacga ggatataaat
     3601 gacgttgcac cggttgcggt tgacggaaag ttcggatata taagaaaagg cggggagttt
     3661 gtgactactc cgcagtacag ctttgcgggg agcttttcgg ataagggact tgctaaggta
     3721 gttgacgaaa acggcaatgt cggatttgtc gacagtgcgg gtcgaaaggc gtttgacggt
     3781 cagatgttcg actatgcctc ggatatgaac gacagcggtt atgcctttgc gtactctctg
     3841 cttatgcctg aaatcacgac cggtacggaa agcggaaact acaacgagat catatacagc
     3901 ctgctgagtt ccgacggaaa agtgaccgag cttgcaaacg gcacggggat atcggcttta
     3961 tacgatgata agtatatcgg tttcagacag gcgcaaagca acaatgaaac aacgcttgat
     4021 tttgcaaggg agtatgccgt gttctcggcg gacgaaacgg cgcttaccgg ctattacgac
     4081 cgcatagtaa aaagcggaaa gctgcttatt gtttgcaaag gcacagatga tgacagtaag
     4141 ctgtataagg cgaaactgtt aaaggcggat acgcttgaac aggtcggcgg agattatctg
     4201 tgcggtgctg acataaaagc atacgacaat ggtgcggtac tgctcaagag agatgataac
     4261 gactttataa gaggtgttct gatcgatgat aacggcaacg aggtctttgt gtcatacgac
     4321 ggagcacata tagcaagcgg atttgatctg agcggatatg cgtgtgttta cgaaaaagga
     4381 aaatactgcg gatatacggc tgaaggcaag cagtttgaaa atgattttca atttggtgag
     4441 ttcaactgcg gacttgcacc ttactatgac aatgcgaaaa tcggatatat caatacggca
     4501 ggcgaaaagg tgacagacgc taagtatgac ggcgcaagcg gatattatgc ggacggttat
     4561 gcttatatta agagcggtac ggaatattcc gtaatagata tgagc
//
LOCUS       DAWAOG010000100         4591 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2423_length_4591_cov_2.364418, whole genome shotgun sequence.
ACCESSION   DAWAOG010000100 DAWAOG010000000
VERSION     DAWAOG010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2423_length_4591_cov_2.364418"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1504 a          928 c         1088 g         1071 t
ORIGIN      
        1 tagcaggact tactgcggca gtaatttcgt ttacgcttac aggtgtagcg gaaaatgcaa
       61 tgagcggagc aatgcgcagt gatgatatca tatgtgggat caaaaggctg acaatgtaca
      121 gcgcaacatt ggctatgccg ataaatgaat ttgacacaaa cgaaagtaca cagaccgatg
      181 ataccgtttc gcaggacaag cacgaaacct ccgccgagga aacatcgtca gtggacagta
      241 ttgaagaagt cccgcaaagc gagagcgtga cctcatcaaa tacaccgagc gttatggaaa
      301 gcgagccgac agagtcgctt gtcccggcgg cggctcatgt ggaaagcaag acgggtctta
      361 tttcgctgaa tatcagaaaa gataaagacg accttaacgg ttacatggca aaagacggta
      421 tcatacagga aaagcactac ggcaagatca caggcgacaa tgcaatagat ttaaaacacg
      481 gtcaggtgcg taactgcacc gaaatgccgt caaatgtttt gctgaccgag ggtacaagaa
      541 aaccgggtat caggatagaa agcggcagtg acccacaggt gctgataata catactcata
      601 ccaccgaaag ctatgagcgt aacagtgacg gatattacga tacctcctat acgggacgtt
      661 cgctgtgtcc cgcaaacagt gttgtcggag tgggagcggt tcttgcgcag acgcttgccg
      721 acaacggcat aagcacaatt catgacggta cggtttttga tgatccggta tacaattatt
      781 catattcccg aagcagggag cgcatagcag agatacttaa agagtatccg tcaataaagg
      841 tggtacttga tatccaccgt gacggaatag cggacggaga tgtcagaatt gctccggtga
      901 cggagataaa cggcaaaaaa gccgcgcaga taatgataat atgcggttgt gacgatggca
      961 cgggaatatt gccggattat atacataatc tcagatttgc aacctatctg caaaacggca
     1021 tcgaagccga taatgagggg attacacgtc ctatgctgtt tgattatcgc ttttataatc
     1081 aggatcttac gagggggtcg cttgtcatcg agttcggagc gcttgcaaac gatataagtc
     1141 aggtcagata ttccgcagag cttgcgggaa aaagcattgc acgcctgcta ggagagctga
     1201 tgtaaaaatg taaacattgt gcaattttca caattacttt acttctgttt tgtaagaagc
     1261 ggcatagaca aatttctcag aatatgttat aataacccca gacataaaat ccgctgaaag
     1321 cggattttgc cgtgctttgc acggcttgcg gcacaccgca agtctgagaa cattttaaca
     1381 taaaaacttt tgccgacagg caaaagacgg cgtaccgccg tgtcggctta cgccgtcttg
     1441 attttatgtt ataatatatt aaatgcatta tactgtaatg cgttaaacaa taacttatga
     1501 gagggaagag caatgaaaaa ttatcttaaa cgaccgcttg cggggatact tgctttatcg
     1561 ctttgctttg gctctgtatc tttacaggcg tttgcagacg agcttgagtg gggaacggcg
     1621 cagacagaaa tagcgatggc aataggcgat gttcagcgtc cgtctgaaaa cgtgataatc
     1681 accacggccg acggtaatta tcctgtaaat gacgaaacaa ctatatacat agataaaggt
     1741 acatcgtgtg aatttaattt gcggatcggc gacggtgtcg aaaactgtga cgacagcatt
     1801 atattaggta tagccgatga tgcagagaag aaaatagaat gcaaacggaa gcccacagat
     1861 gaccctttac aggaaacgct tactgttaag ggcataactc cgggttcata ttcagttgta
     1921 gttgcaacaa aaagcggtaa agcaaatcag aaaataaatt tagttgtact tagtccgctg
     1981 acagaagcaa agatcaaaaa cggcaacaaa gatataaccg gaagcatagg cggggttatg
     2041 ataagggata atcagcggct ccagcttaaa atcgcaaata ctccgtcaaa cacgaccgat
     2101 accatagtgt ggagtgtcag cgacactgct aaggctaaga tatcttccga cggtgtgctc
     2161 accgctaaaa gtgcaggcac gataacggta actgcatcgg ctgtaaatcc gtattatgat
     2221 aaatacaact atcccgcaca gcagctcaaa gcatacggcc ttaatcagag aacatacagt
     2281 tgctcgacta ctattactat acttgagtca aacgcgatga agaatcttcg ctttaaagac
     2341 gaaaacggta atacggtaag ttcgatcaac ctgaacacag gcgataagta tgacgccagc
     2401 agcattctta aatacgatgt ggaaaaagac gggaactctt ctaccgatga aataatatgg
     2461 cagtcaagtg atacatcggt agttgcggta gatgaaaaga ccggtatgct gaccgcgcag
     2521 aacaagagcg gctttgctac tgttacggca atgaccaaca gcgcagatcc tgccaaggct
     2581 tcgtttgttg taaatgtatc ttcccctgca actgttcttt cattcagcag caataagtat
     2641 atgatggata tggatggaag aaatgagtat gatataatag cgatcgaatc gcctaatacg
     2701 gcagatgaac ctctggtatg cgaagtttca aaagacgtaa acggcaatga cattgcagag
     2761 gttatcagaa taacggacgg aaagactcca aacataaaaa taatcagact taaggctctt
     2821 aagaccggta cggcaaaaat cacggtaagt accgacagaa ttgcttcaga tgatggtgaa
     2881 gcgcccgaga atatctcggc aacctgtgag ctgcaggtcg tagactctat agagtcatat
     2941 gcctcattca aaattaaaga gcctatagcc gatgttgtat ataacggcac ggttcagcgc
     3001 cttaagccga tagttacgga tgccgcagga aacgagctta cagaggataa ggactatact
     3061 cttaaataca caggcgacac atttaatgca ggactggtta ccgtacgtgt tgcaggtata
     3121 gagtctgccg cacagtacgg aatgatcgat gtgacatatc agatacttcc tgccgatatc
     3181 agtgagataa caaaaccgac tttaagcaat atgacttata acttcggcag tgagctcaaa
     3241 cctactgtac cggcgatcaa gtatcgcaat ctttcgctca ttcggaatac tgattttacc
     3301 gtagagtaca taaataatat caatgtaggt acagcaacgg caataatcac cggtaagggc
     3361 aactataccg gcagcattaa ggcagactat gaaattcagc ctaaaagcat atccggtgca
     3421 caggtaagca gtgttgctcc gatcacatac accggtgacg aaataacccc cgattatacg
     3481 ctgacaatga acggcaacaa gaccactctt gttgaaaatg aagactatac agtcgaattc
     3541 agcaacaatg taaatgtggg taatgcaagc gttgtattta caggtatcgg aaactatacc
     3601 ggtgtaacat ctaaaaaaac tttcaaaata gcaaagaaga atgtaaccga agacaatgaa
     3661 gtaagcgtca gcgatatacc tgatcaggga tatatgggcg gtatgcctgt tgcgcctgag
     3721 ctgacagtaa cttataacgg cagaacgctt acagagtata ttgattatac attgaaattt
     3781 tccgacaact gtaatcccgg caacgaagca tcggttgtta ttgatttcag taaatcgctg
     3841 aatttcaccg gatcactcaa gagatttttc gctatcgtga acagagatgt cgaaaaaccg
     3901 ggcaagggca taaaggcatt tgataaaaac ggcaaggaaa tagcaaatga cgctgtgctt
     3961 tatgtagatg taaaagaagt tatggacatt gatatcgcta tagatgatgc cgataacacc
     4021 gatgactgtc tgctggtggc tatacagtca agcaaccaga atgccggcgt agcatttact
     4081 aagtattcaa gggacggtaa aacagcaact gtcagtgtaa caggtaaaaa acagggctct
     4141 tttgaaatgc agatctcatc gctcagcggt gaaatgaata agaagcttaa gtttacaata
     4201 cttcagcctg ccgagaagct tgaaatttat aacggtaaaa ataaggtcac caatggtggc
     4261 gtatttgtgg cagaaaatca tcagacggca tttactgtaa aatacactcc gagcaacacg
     4321 accgataaag tagaatggtt cgttgacgat acggaaaaag ccgagatatc caaggatgag
     4381 ggtatattga cagccaagaa ggcgggcact gtaatagtta ccgctaaggt gaagccttcc
     4441 gaaaattcag atagaggctt aagagtcaca acaacggtaa cggttgttaa ggcaaaccct
     4501 ataaccgagc ttaatttccc gtttaaggca attgacctca aggtcggctc aacatatgac
     4561 tgcaatgcgg tcgtaaagaa atctatagag g
//
LOCUS       DAWAOG010000101         4587 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2427_length_4587_cov_2.649603, whole genome shotgun sequence.
ACCESSION   DAWAOG010000101 DAWAOG010000000
VERSION     DAWAOG010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2427_length_4587_cov_2.649603"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4305..4314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          997 a         1226 c          919 g         1435 t
ORIGIN      
        1 gtatagcgca ataagagcac ttgtttgaac agccgtccgc tattcttaag tatgcatagt
       61 ggggcagagt agactgcagc ttgtcgccct ccatattatg gcacagcttg tcgccgaatg
      121 tcacgaccct tttatccagc attaccgagt tgatgatctc aactatatca tcgttctttg
      181 caatgcctac cactgcatct atttccggga actcttcgtc catctgcttc tgataacgct
      241 ccgcaaggca acccgttacg attatcttct tgttgatacc gtcattcttg cggctgatag
      301 cttccattat ctcctcgata gcttcttctt tagcgctttg gataaagccg caggtattga
      361 ttattacaac atctgcaaga cccgcctcgt ttactatttt gaagccggct ttagctatct
      421 ttgcaagcat aagttcggca tcgcactggt tcttcggaca accgagagat accataccga
      481 cttttattgt gtctgccatt atttttcctt acctttccgt ataccgtatg tattatattt
      541 tcgcgattta ctcatcgtaa tattcttctt cgtcataatc tccgtctttc acgcgggata
      601 atgctagctt tatatcatca tatgcaaagc ccctgcgcgc aagtgccgca gtcacacgct
      661 gaacgctctt atagtcagaa aaatccagct tgtttgcata tttcttatct acaagatgta
      721 taatttcgtc tataaaatcc tcagcgtcat attcttcttc ctgctgtgaa ataaggctgt
      781 cgggcaagcc ctttctccgt agctcaaagc ggatcttgga cttaccccaa cccttgttta
      841 tatatgcctt gtaaagcttt tgtgtgtact tctcctcatt tatgaggtcg tgctcacaca
      901 tcagctcaac taccatctcg catagatcgg gaggatagtt ttttatcagc ttatcataaa
      961 gctctttttt cgagtgatca cgctgagcga gtatgtacag cgctctgcgt tttgctctta
     1021 ttctttcatc atcggtatat gacatcagtc aagatcctct ggtgaaaatt cttcaaagtc
     1081 gctgtctgct tcgactacta cgcttttctt tgctgtcgtt ttctttttcg ttgacggtgc
     1141 ggattttgcg ggagcgtcag cctttgcagc ctcttcaccg atctccttaa gctcgttttc
     1201 tgccgccgat tctgaagtgt cggcattgtc cgtgccttct ccgtcctcgc ttgccgacat
     1261 atcgagctta gacatattct cttttatctt cccttcgatc tcaagacgca gatcctcatt
     1321 ttccttaagg aacttctttg tcttttcctt accctgtccg aggcgttcgc cgttatagct
     1381 gtaccatgcg ccgctcttct tgataaagcc gagctcttcg cccattgtta ttatttcgcc
     1441 gagccttgaa ataccctcac cgaatatcat atcgaactgt gtttccttga aaggcggtgc
     1501 gaccttgttc tttacgactt tgcacttagt agtataacct attatctcgc catcgtcaac
     1561 tatcttctca cccttgcgga cctctatacg cacactggca tagaacttaa gcgcacgacc
     1621 gccgggagtt gtttcggggt tgccgtacat aacgcctatc ttttcacgaa cctggttgat
     1681 aaagattgca acggtgttag actttgatat aacgcttgtc agctttctca tagctgccga
     1741 cataagtctt gcctgaacac cgacaaagct gtcgcctatc tcgccgtcga tttctgcctt
     1801 tgtgaccata gcggcaaccg agtcaagtac gacaatatca atagcacccg agcgtgcaag
     1861 cgtttccata atttcaagtg cctgctcgcc cgtatcgggc tgagatacga taaggtggtc
     1921 aatatcaacg ccgagcgact ttgcgtaaac ggggtcgagt gcgtgctcaa cgtcaatgaa
     1981 tgccgccata ccgccgagct tctgcgtttc ggcgatggca tggagcgtaa ctgtagtctt
     2041 accgccggac tccggaccgt atatctcaat aattctgccc tttggcagac cgcctatgcc
     2101 gagtgcatag tcaagaccga tcgatcctgt cggtacgctt tcaacatcaa gctttttgtt
     2161 cccctcgccg ccgagaaaca taaccgaacc ctgaccgtag ttcttgatta tctgttccat
     2221 agcggtatca agagcctttt tcttttcttc cggagtggta gggtgattta cggtaggtgt
     2281 cgcttttttg ttatttgcct ttgccataat agaattacct gtcctttcag atttcaatat
     2341 gtatcggttt tattctgtga ttatatttta tcataattgc tgtaccataa aatgtacaga
     2401 ttttattttc tatcgcttaa cagcacagac tattctgtcg tgcccgctgt agtccttaac
     2461 aacctgcgga gtaagtcccg ctttttcaaa tattccgctc acagcctgcc cctgttcctc
     2521 gccgatctct accgcaaaca gtcctccgtc tgcaagcttt tctgcccaca gcggcacaag
     2581 cccacgataa aagtccagtc cgtcctcgcc cccgaacagc gcaagctccg gctcgaatgt
     2641 tacttccttt tgaagagttt tcatatccgc agagcttata tacggcggat ttgaaagcac
     2701 ggcagacaat gaattatctg caagtctgtc gagtacatct ttatcaaaga tatcgccgtt
     2761 tatagctgtg acaagctttt gggctttgtt ttccgctatg tttttaagaa agtagccgta
     2821 tgccttgtcg gatatctcga tacccacggc ttcgacattt gcttcaagtg ccgttgtaac
     2881 agctatacag ccgctccccg agcacaagtc gatcaccttg ccgcctgtct ttttaagatg
     2941 ttcgattgct atatcacata gcagctctgt ttcagggcgg ggaataagca cgccctcgcc
     3001 gacaaagaat tttctgccgt agaagtccca gctgcccagg atatactgta acggctttcc
     3061 cgttttgcgc atatccgcct ttttgacaca ctcggtaaac tctgcttcgg agatatcctc
     3121 aaacggcatt ttcccgccgt cgacctcact tacgatctgc tttgcttcaa attccgcatc
     3181 atcggtaatg ctttcaagca tatcgcttat aagccgtacc gcctgcctta ttaccacttc
     3241 gcgtcctccg gcttttccgg gagaacgggc ttcatagagg ctatcgcaac ctcgatcatc
     3301 tcatcggcag gctcttttgt tgtaagacgt tggatagcaa gtccgggagc tgaaattatt
     3361 tttgtaacca cattatcgta tcttcctgcg atacggataa gttcgtatga gatacctacg
     3421 actatcggca ggaaaattat cttacagcaa attctcagca tatcgcctat aaattttgat
     3481 acgccgaacg cttcaatgaa cagctcgctg tttatcggga gcagggtata taccagtatg
     3541 cttatcgcaa gcgtcagaaa tataaagctt gtaccgcatc tggggtggaa gcgcttgtac
     3601 tttctgacat tctctaccgt cagctcatca ccgtgttcat agcagaatat cgtcttgtgc
     3661 tcagcaccgt gatattgata cagccttttc atagtcttca taagcgaagt cagccacaga
     3721 tagccgacaa acagtgcgat cttaataata ccctctgtca gcgagcggaa ggcagatatg
     3781 tccgtatcgc cgaacagtgt ctgtatgcct gtaaagataa gcgtaggcag tatcacaaac
     3841 agcgcgacgg caagccccac gccgagtacg cccgatatta ttgtgacagc cgttgtcagg
     3901 gcggcattat cgccgtcttt cttttcgtcc tttttatctt ccggtttttc tttgcttacg
     3961 ttatctgccg gtttgtcctg ctctttttct tttttcttct tgtctttttc gtcctcttct
     4021 tcatcgaaca ttccgctcac ttcgcccgac ttgttaaggt acttgtagcc gtctgccaac
     4081 tgtgaaacga aattcacaca gcctctgata aaaggtgctt tgttatacca ttcaggcttt
     4141 ttcctgtccc acacctcggt atatatttct ctgtcgggct gacgtacagc catacagccc
     4201 tttttcagac cgagcatcat aacgccctca atgagagcct gtcctccgac ctttgttttg
     4261 attatctcct tgttttgttc gctcatattc agcctttctt acagnnnnnn nnnncgtcct
     4321 ttttcgctat cggcagggtg ataactaccc ttgtataccg tccgagctcg ctttctatat
     4381 caagcgttcc gccgtgcgct gtcactatct catctgcgac agcaagtcct attcccgagc
     4441 ccctgaccgt gttattcgcc ttgtagaatt tagcctttac ctttgccaga tccgactcgg
     4501 caatgccgca accgtcatct tccacggtaa ttattaccgt gtcgggcgtt accgccgtgg
     4561 caacgatgat atgaccattg tcggcgg
//
LOCUS       DAWAOG010000102         4580 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2433_length_4580_cov_2.466961, whole genome shotgun sequence.
ACCESSION   DAWAOG010000102 DAWAOG010000000
VERSION     DAWAOG010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2433_length_4580_cov_2.466961"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          984 a         1217 c          930 g         1449 t
ORIGIN      
        1 gtatagcaca gtgtcgggtg aatttcaagg tttaaaggat attttacacc gactgccgct
       61 tacggattta cacaaatttc agctgatcga tatcctcatc atacagcgtt cccgcaacag
      121 gcacagtctt tgtcctgtag ctgtcctcgt cctttatcat tccgtcctca tacttaacag
      181 cggagctgag ctttgctttt gtgagcctca ttacctgtac accctgcgta tctcttgccg
      241 ctttgggcag gataagcacg gtgttgaata tcatgacctt tcctgcgtcc gagcttagca
      301 taaactcgca atctccgtca ataagcgtca tagatacaag cggcgagccg tcattgtatg
      361 catttgcaag ctttttacgt cttgtctttg tttcaaacga tgccataggc actttagcac
      421 atttaccgtt ttcaaagaac atcacaagcg ttccgctgta gtcctctgcg gccaccatct
      481 ttattatcct ctcgtccggg tcaagctcca gctttacagg cagatagtcg cccattacgc
      541 ttgccttgcc gtcctcaaag tcacaggctc tcgccttgta cacctgatat ttatcagtga
      601 agaacaacag ctccgcgcgg ttcgtgccct catatgtcgc tattatctca tcgttttctt
      661 taagcttctg ttcgccgctc attctcagcg actgcggagt gattttcttg aaatatccct
      721 ctctggtgaa gaacagatta acgggataat cgggtatttc ctccgctacc tcgttttcct
      781 cgtcctccgc accgtagatg aacattgtct ttctgggctt gccatacttc tttgctatat
      841 cgccaagctc ggaaattatt atctgcttta ctctgcgctc cgaagaaaga atatcctcca
      901 tatcggctat ctcttcctta aggctgtcta tttcctcgat acgctttaat atatagccct
      961 tgttgatatt tctgagcttt atatccgcaa catattcagc ctgtgtttcg tctataccga
     1021 agcctatcat aaggttcggt acaacctctg catcgtcctc tgtttcacgg attatcttta
     1081 tagccttgtc tatatcaagc agtatcttct taagacctgt cagcagatga agcttttctt
     1141 tcttccttgc aagctcaaac tttgtctttc tctttacgca ggtgattctg aaatccgtcc
     1201 actgctcaag aatctcggca acgcccatga ccttcggcat accgcctaca agaatgttga
     1261 agttgcagga gaaaacgtcc tgtaaaggag ttattctgaa cagctttttc attaccgcct
     1321 cgctgtcctg cccacgcttt aaatcaatag ttatcttaag acccgataag tccgactcgt
     1381 cacgcacatc ggatatctcc ttcagcttgc cgagctttac ctgctccact atcttgtcga
     1441 ttattgcctc gatagtagtt gtaggcggta tctcggtcac ttcgatacag tttgccgcct
     1501 tgtcatagat atacttactg cgcaccttga cgcttccgcg tccggtagca tatatacggc
     1561 gcagctcgtt ctcgtcctgt aatataaacc cgccgccggg aaaatcagga cctttaaggg
     1621 tggatacaat atcgtgttcg ggatttttca tcagcgcggc ggcagtttcg cacacctccg
     1681 acaggttgaa cgagcagata ttgcttgcca tagatacggc gataccgaca ttattgttta
     1741 ccagtaccgc aggaaatctt gtagggagca gtacaggctc tgtcatcgag ttatcgtagt
     1801 tcggcacaaa gtcaactata tcacggtcga tatccgcaaa tatctccgcg cagatagggt
     1861 cgagcttagc ctccgtatat cgtgatgccg caaacgacat atcccttgaa tacgccttgc
     1921 cgaagttacc cttgctgtca acgtacgggt gcagaagcgc ctcgttgccc cttgcaagtc
     1981 ttaccatcgt ttcgtatatt gccatatcgc cgtgcggatt taatttcatc gtctgtccga
     2041 caatgttcgc cgactttgtt ctcgctcccg taagcagacc catcttgtac atagtataca
     2101 gcagttttct gtgggatggc ttgaaaccgt caatttcggg gagcgcacgg gaaactataa
     2161 cgctcatcgc atacggcata tagttttcgg tcagcgtatc ggttatcggt tgattttcga
     2221 caatgcctgc gccctctata taagcgtttt tattggaagc gattttcttc tcgctctttt
     2281 cgttctttcc ttttctcaaa atctatacct ctttttagct ttatgccctg ctcagagcga
     2341 aaactctttt ttcagctttt caaagcgttt ttcatattta tctcttatcg gtagcgttgt
     2401 attctgcaaa acgtgctgta aaatatctgc gtctttcagc acctcgtcaa gcggaagccc
     2461 gacattttcc ttgtcgctgt gatttgcaat agctcttgtg atattgccta tctcgcacac
     2521 gctgaaaagc cctgtctttg cgagtatcgg cattactgcg tcagcacttt cctctgcgtg
     2581 cttttcgtca acgccggtca gatatttacg gtagtcgtgg agcagtccgc ttatctgtgc
     2641 aagctccgcg ttcacacctc tgcgcagtgc gataagcccc gccgtctgcg ccgtaccgaa
     2701 aagatgtaca tacgctttct gctgtctttc ctcgggcagt gtcgctatca tctccgatac
     2761 gaccgccgat attacctcta tcctgttgat ctccatatat cctccgtctt acgatacgtc
     2821 tatatcatca aggtacttat agccgtagtc gcggatatag tcctttcgtc cgtcaaggtt
     2881 atcaccgagc agaagctcga atatctcctg agttttctgc gcgtcctccg gcattactct
     2941 tataagccgt cttgtttcgg ggttcatagt cgtcagcgac atcatttccg gttcgttctc
     3001 accaagaccc tttgaacgct gtatcttata cttctgctcg cctatatcgg caagtattct
     3061 ctgcttttcc gcttccgtat aggcaaagta ggtcatatcc ttactgttta tctcataaag
     3121 cggcgactct gcgatatata catatccctg ctcaattacg gtcggaacaa gtctgtacag
     3181 catggtaaga atgagcgttc ttatctggta tccgtcatag tccgcatcgg tgcagataat
     3241 tatcttttcc catctgagat tattcagatc aaaggtcgaa aggtctttgt tcgccttagc
     3301 ctttacctcg acaccacagc ccagtatctt cataagattt gtgatgatct cgctcttgaa
     3361 tatcttatca tagcttgcct taagacagtt gagcgtctta cctcttacag gcattaccgc
     3421 ctgatagtct gcgtccctcg cctgctttac agcgcctaaa gccgagtcgc cctccactat
     3481 ataaagctca cgtcttgccg tgtccttact gcggcagtca acgaacttcg gtattctgtt
     3541 tgcaaggtct atcttgcccg aaagctgttt ctttattgta tccctcgcct ttgcggcatt
     3601 ttcacgggca cgcttgttta tcagcacctg ctcgcatatc ttgaccgctt cgtccgggtt
     3661 ttcaaggaag tagacttcaa gctgatgctt cagccagtcg gtcatagcct cctggataaa
     3721 tttattggtt atgctctttt tcgtctggtt ctcatagctt gtctgcgttg agaagtttga
     3781 agatataaac acaaggcagt cggcaatatc ggtaaatttt atcttgccct cggtcttgtt
     3841 gtacttgctg ttggctttca gccagccgtc tatctgattt gtgaatgccg cctgcatagc
     3901 cttgtcggga cttccgccgt gctcaagcca gcttgaattg tggtagtgct gtataagctg
     3961 taccttattt gagaatacaa aagcgacctt catctttacc ttgtattccg gcttatcctc
     4021 tctgtcctta cctgtacgct gtgcgctcca tacctgctcg gtcgtgagtg ccgtatcacc
     4081 tactatttct ttaaggtagt cgctgatacc gttctcatag cagtagatat gctcttcaaa
     4141 gcgtgtttac cctgtttcct cgtcacgcac ttcgtttctg agcacaaaag tcagcccgtc
     4201 atttacaact gcctgtctgc gcagaacatc gtcaaaatat tcaaacggta catctatatc
     4261 ggtgaatacg tcaaggtcag gcttccactt gattttagta ccagttgttc cgtccgactg
     4321 gtgacggcgg aaaccgcgct cttccttttc aacgttatag cccttttcaa atctcaggtc
     4381 gtatatccat gtgccgttgt tactctcgac ctccatatac tcagccgcaa actgtgtcga
     4441 gcaaagaccc agaccgttaa gaccgagcga atatgagtag ttatctccgc tgttgttgtc
     4501 gtacttaccg cctgcgtaca gtgtgcagaa ggcaagctcc cagttgtact tttcctcgtt
     4561 tttattgaag tctatcggta
//
LOCUS       DAWAOG010000103         4579 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2435_length_4579_cov_1.983864, whole genome shotgun sequence.
ACCESSION   DAWAOG010000103 DAWAOG010000000
VERSION     DAWAOG010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2435_length_4579_cov_1.983864"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1514..1523
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1448 a          792 c         1077 g         1252 t
ORIGIN      
        1 gtcaagaact ttttgaaaaa agttcttcag agtaaattcc caaagtaagt tccacagtgg
       61 taatttgagt ggaacttgct ttaataagaa aaatgtggta atattttgtg ttaggaggag
      121 cttacccgga cgaaacgtga attatccgtg aaagttgcgt tgtgtagctt acacggataa
      181 ttacatactt tatcttttta caaaaaattg tgcttttttc tcttgacaaa cactatatgt
      241 tgtggtataa tttaaaaaca acaaacgaat gtacttactg cttttatata cagtaaattg
      301 aaaggaagat aattatggta aaagtaaatg taaagaacgg cacgcttgat cagaaagaga
      361 tagatgccta tgttcagtat gcgattgaag ctaatccggg aaaactggta accgaacttg
      421 atataaatct tgacggtgat tatgttgatc ttacatatca tttcggtgaa attccgtttg
      481 atcgcatccg ccgtatcaca ggatatcttg ttggtacact tgacagattt aataacgcta
      541 aaaaagctga agttcaggat agagtaaagc acagcattac tcctgaggct gaagaagata
      601 tttatgtcac tgcttaaacc gtcattgttc tccaaaaaat gacagtatat atttaaggct
      661 atccgcccat acttttattg gaaaatgaaa gcatgggcgg tggttttttg tattacaaca
      721 aggttgagat aagcggtgtc aatacagcaa aattaaaggt gctcaaggag tctgaaaaga
      781 tggaacttct gcaaaaaatg cgtgacggtg atcctaatgc cagagaagag cttataaacg
      841 gcaatctccg ccttgtactg agcgttatac agcgattttc cggcaggggg gagaacgctg
      901 acgatctgtt tcaggtcggc tgtatagggc ttatgaaagc aattgacaat ttttgccctg
      961 attttaatgt caggttcagt acgtatgccg tgcctatgat aatgggcgaa ctgcgtcggt
     1021 acttgcgcga ttccggctcg atccgtgtca gccgctcaat gcgggacctt gcatataaag
     1081 ctatgcaggc aagggagaag ctgactaacg aacactcgaa agaaccgacg atagagcaga
     1141 tagcaaagga gatcgacgca aagcgtgagg acgtagtgat tgcacttgag gcagtaagcg
     1201 atccgatatc gctgtatgag cctgtttttt ctgaatcggg cgacacaata tatatgatgg
     1261 atcagatagg cgacaataac gatgataaga actggcttga cgaaattgct ttgaaagagg
     1321 caataatagg gcttgatgac agagaaaaga atattcttaa actccgcttt ttccgtggaa
     1381 aaacacaggt agaagtagca caggaaatag gtatcagcca ggcgcaggta tcaaggctcg
     1441 aaaaaggcgc gctcggtaaa ataaaaaaag cattataaac taaaaatttc aaacatattg
     1501 acttgtgcaa ttannnnnnn nnnttttttg tttacattta ttgaaaaccc accttttata
     1561 tgttatactg aaaacagggg tgagcgtatg aaacgtaaaa taaacagaaa tacgatattg
     1621 aatattgcga tcgttgtact tgtagtaata tgtgcggcgc tgtgtcggtg ctttgacaga
     1681 cttgccgaaa ttcctgtatt acggatctgc agaagcttaa tatacatcgt attgtttttg
     1741 tggtggggag ttatgctaag aaaacgtata gtgcaggtgc aggcaaagcg gtttatgaca
     1801 gccatttcgt ttcttatagt tttttgggta ctggtcagaa ccgtaaaatt catttttgtt
     1861 gaaaacccta cagttatccg cttgctgtgg tattcttact atatacctat gatatttatt
     1921 cccgtgcttt cgcttttagt tactctttca ttggaaaaat cggaaaatta tcaaatatcc
     1981 ggagttaaga tattgttgtt ttcattttct gccatactgg ttgcaacagt gcttacaaac
     2041 gattatcatt gcttcgtttt caagttcccg gaaaatgtac aggtgtggag cgacagtgat
     2101 tacagttatt cgttcggata ctgcattatt gctattttta tacttttctg cactgtcagc
     2161 gctcttgcgg tgttgatata caaatgccgc ataccaaaga cgaaaaaaat gatcgttctg
     2221 cctgtcattc ctgtagctct gatggtaata tacagtgttt tatatgcttg cggtgcaata
     2281 tcaagcggca cgatattgca taagatctcg ggtgatatga cgattacagc aagtctgctg
     2341 ataatgctta catttgaatg ttgtatttac agtgggctga ttcgttccaa tacccattat
     2401 atcgaattgt tatgtccgtg tacgcttcag acgctgataa ccgatgatga ttacaatgtt
     2461 ttgctggcat cggactgtgc ggaaatgctg tctaaagagg agatgaggtc tgcggaaaaa
     2521 atgccggtaa caataagccg cggtaaacgc ctttcatcaa caaagatcaa gggcggtcat
     2581 gtcctttggt ttgatgatct gtcagaactt gaggagatca acgaaaagct ggctgaaatg
     2641 aaagatgatc tgaaggagga atatgatctt ttatgtgaag aatatgagct taagaacaga
     2701 gaagcccata ttcttgaaag agataggata tacgaccgaa tacagaaaga taccgcaggt
     2761 cagataaaag ctctgaacag tcttattgac agatttgaat tggaagaaaa tgaaaatgaa
     2821 cgcaaaaagc ttcttggcag aatggtgata ataggcgcat atctgaaacg aagaaataat
     2881 ctgatattcc tctccgagaa atccgatatg ataagcgaaa aagagcttta tcttgcattt
     2941 tgcgaactga ttgacaacct tgaattgtat ggcgttacct gtgggcttga tataaaagcg
     3001 gatactcctt tgccatcgtg tactatcact gaaatgtttg atacttttga gaaaattctg
     3061 gagctgtcgc ttgaaagcat aactgcagtc aacgttgtta taaatcgtag cgcaaacgct
     3121 tgtaaaatga ctgtaatatc cgatgccgta tccgatctga gcatattaaa cggcagcagg
     3181 ataagcgcag tgcgcgatga tgacggagag tggcagataa catataccgg ggaggcggaa
     3241 aaatgtttga cataggcaga aaattcaggc tcagaagagc tgtaaaagaa gcattcgata
     3301 atcttcctat cggtatatgc ttttttgaca gaaacggtat ggttacatta tgtaatacac
     3361 agatgaataa gcttgtgtat gagatcagcg ggaacgatat ccggtatata gacgacatat
     3421 ttaatctgct cggtgaagaa agaagctcgg gcggcacagt cattatggtc gacggtaaag
     3481 catggcgttt cagaccggat atcgttattg atgaatacga agactgttat acgcagataa
     3541 ccgctacaga tgtagacgaa ttgtatgcat acaggttaaa actcgaagaa acaagcagaa
     3601 agctcgaacg ggcgggcgag cgtatgagaa agctgaccga gaacatcaga acggtcatac
     3661 gcgaggaaga aatactaggt atgaagattc gtgtacacga cgatataggc aggagcgtga
     3721 tcgcgacccg cagtttgctt aacaataacc gtccgacaaa tgagcttgac cttactgtgt
     3781 ggaaaaatgc ggtcaggctt cttatgaggg ataacgaaaa gaacagcggt acatacagct
     3841 atgatatatc ggaacagctg aaagaaacgt cttctgttct cgggattgaa ataaatatta
     3901 ccggagatat tcccgattta ttttataaca agatattcat cacagcggtc agagagtgtg
     3961 cgtcaaatgc agtacgtcat gcaggcgcag caaagctgaa cgtaaaatgc agtaaatgcg
     4021 gcggaagaca atggatagcc ttgtcaaatc acggaagagc tccggatagt accgttacgg
     4081 agggcggcgg tctttcatcg cttcgtgaca gaatagaaaa aagcggcgga acaatgaaca
     4141 taaattcata tccgtttttt gagcttgtaa tatctttccc gcttaaaaag gaggtaacgc
     4201 tatgacgcag gtactgatag ttgaagatca gaaaatgata agggaaagcc ttgaaaatat
     4261 ggttacacag acagacggcc tcagacttgc aggctctctg tcctgtgctg cacttgccga
     4321 gcaatactgt atgcggagcg atatcgacct gatacttatg gatatttgca cagaaaatga
     4381 cgaaagcgga tttgtcgctg cagaaaagat aaagaaacgc tttccgacaa taaaaataat
     4441 catcataaca tctatgcttg attactgcta tcttgataag gcgaggaatg ttggggctga
     4501 aagcatctgg ttcaaagatg caagcgcaga agagctgctc agcgttataa cgaggacgat
     4561 agacggggag tgcgtatat
//
LOCUS       DAWAOG010000104         4527 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2462_length_4527_cov_2.272585, whole genome shotgun sequence.
ACCESSION   DAWAOG010000104 DAWAOG010000000
VERSION     DAWAOG010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2462_length_4527_cov_2.272585"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1452 a          914 c         1160 g         1001 t
ORIGIN      
        1 acattccctt ctgtcaaaaa aatcttgtca aactatcata aagttatgga attttccatc
       61 aaaatgtgtt acaatattag ttaagctatc tgcggctgcc gcttttgcgg aaaaagcagt
      121 tatgatttta ttttatttta tgttccgccg cggcggagaa aagaaaggac gtaatcaaaa
      181 tggcaaagga actcaattta caggacgtat ttttaaatca ggcaagaaaa gagaagatac
      241 ctgtaactat ctacatcacg aacggttttc agttcaaggg acttgtaaga ggatttgaca
      301 actacactgt tatccttgac acagacggaa aacagaacct gatatacaag cacgccatct
      361 cgacaataac accgctcaga gccgtatcga tccttgatgc atttgacaag ggcgacagcg
      421 atactgaagt caaagaataa ggatatatgg acaggacatt aacgacaaag ctcataactg
      481 tcgtgcttgc tattttcctg ctggtaacgg tcgtgagcca gttctttgtc ggcggtgagc
      541 attcatacag gaccgaagtc gcactgagat atgattctca ggatactata gagttctatg
      601 gcgtgtttgc cagaaatgag cacacggtaa gccgcaaaac aaacggtatc atacagtatg
      661 cgcacgagga cggatcaaag gaaagaacag tgtgatcgca aatacctaca gctcggcttc
      721 cgatatcaca cttactgccg atatcacaaa tctgcagaaa aggatagata cccttaccga
      781 tgcacagagc cttgcaggca cggataactc acagatcgaa gcatttgaca agcttataac
      841 cgaaaagcac agcaagctta tacaggcgat aaataacgga gattattctg cggttgccga
      901 tataaagtat gagcttctca atcttcagag taagcgcgat atggtaaagg gcaaggtgtc
      961 ggattattct tcggtgatat cggcgctgaa cgcaaaaata aaaacgcttg aatcgaggaa
     1021 atcggcagag cctgtcagcg tattgtctga ggaaacaggt tattttgtaa gcagtatcga
     1081 cggatatgaa agcaagataa cctatgacaa tatcgatacg atccctcccg ccgagatcgc
     1141 cgaagcggta aaaacgccga agacagcatc ggcatcgggc gatattatcg gtaagatgat
     1201 cgaggactac aagtggaagc ttgcggctac ctttacagaa ggacaggccg ccgtgcttac
     1261 cgaaaataca aatgtgaaga tcattgtagg cgcagaccag tcggagataa ccgctaaggt
     1321 tgagaatgtc gagaaatacg gtgatgatta tgtagcggta ttttcggcag acgatctcaa
     1381 ttctactatt gcgtcatcac gcgtagggcg tttcaaactg cttgttgaca gctataacgg
     1441 catacgcata gcttcatcgg cgatacactt cgataaggat aacaatatcg gtgtgtatat
     1501 caagagcggc acacaggcac agtttgtaaa gatagagcgc atttattcaa ccgaggatta
     1561 tgttattgtc cgtgatacaa cagataagag cggctatctg tcgctttatg acaacgttat
     1621 agttgaaggg aaggatcttt atgacggaaa gaacctctaa atccgccgaa gaaaccacac
     1681 ttgatgacat aaggctgaac tatgaggctg taaagcagaa cgtggcagag gctatggata
     1741 aggcaaaccg catcgataaa gtacgcatta tggcggttac taaaacgata ccgtgtgaga
     1801 agataaatta tgcagagagc cttggtatag acctgctcgg cgagaaccgt gtacaggaat
     1861 ttctcggaaa atacgaaaat tattcggaaa aatccgaaat tcacttcatt gggggcttgc
     1921 aaaaaaacaa agtaaagtat ataattgata aggtgagtat gatacattcg gtcgacagtg
     1981 cagaccttgc ggctgagata aaccgcaggg cggctcagca cggactggat acggatatat
     2041 tacttgaggt caatataggc ggcgagctgt caaaaggcgg tgtatcgccc gcagagctta
     2101 tcgaaacggc aaaagtcgta tcggagcttg aaaacgtccg catcagagga cttatgacca
     2161 ttccgcccgc agggggcgac gaaaagtatt ttgcggctat gcaggagctt tttgaaaacg
     2221 caaaacagaa atattctttc ctgtccggca tcgacacgtt gtcaatgggt atgtcggggg
     2281 attatgtatc agccgtaaag tacggctcaa caatagtcag gataggcagc ggtctgtttg
     2341 gctacaggaa ttattctata tgaacaaaaa acggaggtaa gaacaatgag cgcattcgag
     2401 tcattcaaaa attcatttaa gaagtggggc agtcccgatg aagaggacga ttacacagca
     2461 gagcccgaaa agcaggaaat gcccgaagct cccgcacgca gttcgggaca gtcttcaaat
     2521 tcaagcaaga tctatactta caatgcaagc acaacactgc accttgttgt aaacagaccc
     2581 aagaagttca gcgatgcagg tgagatagca gagctgtaca agaacaagac aacggttatc
     2641 cttatgttca acaacacaaa caaggataca gcaaaccgtc ttatcgattt cctcggtggt
     2701 gtgagctata tcacgggcgg tgagataaga agaataggcg aaactacata tgtactcgct
     2761 ccttataacg ttgatatttc gggcgagttc atagatgaga taagcaatat ttcaggcgag
     2821 gacatctttg acgaaatagg ctgagatggc tgaacaggat ctgactaaag accttttgaa
     2881 tgcggcgagg gattgcataa gcaattcccg accgtatttt tcccgctttt tgtccgaaag
     2941 cgagcttgct caggtacagg ggatcggttt tccgaaagac attaccgcag tgttctgcgg
     3001 cggtgcggaa aactgcgagc cgctcaggtg ttgccttgga ctttttcctg cgttttattt
     3061 tgataccgac aaggtatgcg ggtatgatgc cgaggtggct gagatgttcc cgataaaggc
     3121 ggtcacactt acttatcgcc gtgaggatac gataactcac cgcgatgtgc tgggctcgct
     3181 tatggcactg ggcatagagc gtgatacggt aggcgatatt ttcgttacgg aaggcgcggc
     3241 ggcggtattt gtgtatgaga aaatagctcc gctgatttcg gacaacctga cgaagatcgg
     3301 cagggtcgga gtaaagataa atgccgatat caaagcaggt tttaccttac ccgaaaggaa
     3361 gtacgaagag ctttcgctgt cggtggcatc gctgcgtctt gacaacattg taaggagcgt
     3421 gacgggtaca tcgcgcgcgg cggcagtcga aaggtatatt atgccgcagc ttgtacagct
     3481 gaacggcacg gtttgcaccg atgtatcaaa acagctttgc gagggcgata tcttttccgt
     3541 gagaggaaag ggcaagttcc gctttgaaaa tatcggcggc acaggcagaa aaggtaatat
     3601 acacataaca gtgaaaaaat acatttagga ggaatttttc cgtgaatgct aaggatatag
     3661 tatcaaagga atttgacaca gtaaagcgcg gcggctatga tgccgcagag gtagatacat
     3721 ttctgaagga cgtatccatc gaatacaaga agatgcagaa tgagaacgag gagcttgaga
     3781 agaagcttga ggttcttgca gaaaagatca gagagtaccg caaggatgag gacgcactca
     3841 gagatgctct tctcattgcc aaaaagcagg gtattgcggt agtcaatgaa gctaaggaag
     3901 atgccgagaa gataagaaag gaagccaagg aaaaggcaga caagactatc aaggacgcag
     3961 atgcgcttgc cgcaaagaag aaggaaattt cagaaaaaaa tcttgcggac gcaaatgcaa
     4021 aggcaaagca gatcgttgac gaagcaaatg caaaggctga cgagatccat acaataatga
     4081 tgcagcagac agaaagagaa cagatcgttc tccagcgcac cagaaaagaa gttcaggatt
     4141 acacgaccaa gatccttgcc gcatacagcg ctcatatcga gaatatcagg aatatgccca
     4201 aggcttgcga aaacgagttt gttatcaaca cggtaaaaga ggttgaagca agaaagcccg
     4261 aggacagcgc attcgcaaag aagcccgcac cccagagcaa gcctgaaccc gcaaagcctg
     4321 ccgagaagaa gccggagcct gtaaaggcgg ctgtcaagac ggaagaaaag ccggctgagg
     4381 tcaaggcaga agtgaaaaag cccgaagagg acaaggcaaa gggtgattct gtgcttttca
     4441 atctgaagaa cgaaaaggac gataagaaag cacccacggc agagcttgaa tttggaaaga
     4501 acaagtaaaa tacatacgaa tttacgt
//
LOCUS       DAWAOG010000105         4503 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2481_length_4503_cov_1.940872, whole genome shotgun sequence.
ACCESSION   DAWAOG010000105 DAWAOG010000000
VERSION     DAWAOG010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2481_length_4503_cov_1.940872"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    503..512
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2237..2246
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2555..2564
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3187..3196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4135..4144
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1036 a         1063 c         1055 g         1299 t
ORIGIN      
        1 taccggttgt aatcgtttta aaaggcggaa tttacagcgg ttcacagccg ccgttagtcc
       61 tcaagccctt tgagtgcctg tatttcttct ttcttaattc ttatcttgcc gtccttgaga
      121 agttttacat cttcacgctt gaaagcagta gcggcggcat cgggattttt gtcaagcttt
      181 accttcagaa cgcctgtaag caggttggta tcctcaacga tacctctgcc ttcggacgtt
      241 tcgacaagcg cgccgacctt aggggttatc gcgagaagct cctcatagca gttctgctca
      301 tacttaagac agcacataag cctgccgcag gtgccggaga tctttgtcgg attaagcgac
      361 agcgactgtt cctttgccat ctttattgat acaggttgaa actctccgag aaaagtcttg
      421 caacagaacg gtctgccgca gataccgagc ccgccgagca tcttcgcctc gtcacggaca
      481 cctatctggc gcagttctat tcnnnnnnnn nntagaacaa tatcttgctg ccgtcaaatg
      541 tgcagtcaat gtcgataggc ttcatatcga gcttgtgctc ggaaattttt ctggtaaaga
      601 ccttcattat ctcctgctct ttttcacggt ttttctgctg tgtttcaagg tctttttcgg
      661 tagctatgcg cataatgctc ttgagcggct gtatgacttt tttgtcatct acttttcggt
      721 ttgcgataac gacctcgccg cactcaattc ctctggcggt ttccacgata acgtgatttc
      781 cgcattctat tttactgccg gagggagaga aatagtatat ctttccgaca tctttaaacc
      841 tgactcctat tatctcagtc atttatattc ctttctaatc ggcgtctatt ccagtacgcc
      901 gaacagctta gcgggcatat atgcctgcat aagcttaacg tttatatttg tgccgtcaag
      961 cagcataagc tccgacagta cttcgtaaag gcggtacagc cttgcctttg taagcttttt
     1021 aagcggcgcg gcctgcgcag aattttcgcc tgtttcaagc tcacgcagag cttctgaaac
     1081 tcgttcgtac agtatagctg tgacagccga cagatcttct cttgttgtac agccggtcag
     1141 gatggcggct gccgcatatt catccttgca cgacagtgcc ccgatcagct tttctgtcgt
     1201 ggcgtacagc tgtatgcgct tttcgtcaga caatatgccg attatcttac cgggattatt
     1261 gccgtacagc ccaacggatc gctttgctgt atctgcctct attccgttat attcaaggca
     1321 ggaaatgcac tcatcctgcg tcatttcgga aacagcgacc gtcacaagcc ttgatatgat
     1381 cgtttcaagg atcgctgaag tgtttgaaca ggtgaaaatg tagcggttgt gctctgacgg
     1441 ctcttctatg gctttgagca ggacattctg ctgttcttcg gtcatctctt cgcagtcctc
     1501 gtaaatgaac acccttaagt taccgtcatt cggcagggta gaggcaaagc ggacattgtc
     1561 gcggatatta tccttggtgt atttttcgcc tttgacgtat attacatcgg cgtgttcacc
     1621 cgcaattata ttcctgcaat tccggcactc gccgcacggt gcgccgtctg tgagtgaaga
     1681 gcacagatac agcatagcgc accattttgc aaaggttcgc tttccaagcc ctttttcacc
     1741 tgtaaacatt attccgtggg gaagtctgtc gctctctgcc atttctgata acagagcggt
     1801 ttcacgctgt tttccgaaca gctttatgct gtctgtatcg cttggcatca gactttctca
     1861 aatctctcta tgttggttac gaaaatagtt gcgccgccta cgaatacttc aacggggaag
     1921 cttgtataac ttcctacgcc gtaagaagcg gcagatggca cgaactgctt tctcttatgt
     1981 gagtgatcct tgattatggc gattatctcg tcgatcttat catcttcaga gcagatgagg
     2041 aatgtggtgt ttcccgacat taaaaagccg cccgtagaag caagctttgt agcctgaaaa
     2101 cctgcctgtg tgagtgatga ggataccgag tgggcatcat cgttgtttac gatagcgtat
     2161 ataagcttca taaagattac cgtcctttct gtagccggtt attcttgtgg gtttgatatt
     2221 caaggttatt atagcannnn nnnnnnatat tatactgtaa cggagatgtc ggctacgccc
     2281 ttgtcgcgaa gcgtccttgc ggcatctatc gttatcgttt cgcccggcat tataagcggt
     2341 atcccgggag gacagggggc ggcggaacaa gcgcaggtct tgccgactgt catcatcacg
     2401 gggagcttta ccttttcgga aaaatacgcc tttgtcggcg aacacgcaga ctgagtcctg
     2461 atatgtatcg ggggggaggg gagtcggcgc acgaatgccg agtgcggagg gttggcggtc
     2521 agcacggttt ttatcgtttc aaaatcaaca tcggnnnnnn nnnnaaaaga agtacgcact
     2581 tgtattcgtc tgcatattcg cattcaatgt cgaactcgct cagcatctga gccatggcat
     2641 aaccggtggt atgcattgct gtgcagtcaa acgtcagctt catatagtcg ctcggggaga
     2701 gggcgtatcc catatcggcg gcgtgttttt tcagcgcggt gactttgctg catatatcgg
     2761 atatggcaag cggcctttcc gtcagtgctc tgttgcatcg gtccaggctg tcaagtatga
     2821 gatagctcgg gcttgatgtg gctataagcg acatcgcttc tttagcctgc atacagtatt
     2881 tttcgtttgc tatgtgaaga tatgcgccgc ctgtcagtac aggaagggtc ttgtgggcac
     2941 tgtccgcgca catatccgca ccgagcgtta tcgggtgtaa atcgacaggc gcaaatttaa
     3001 gatatgctcc gtgagcgttg tcaaccagca gaggtatact gtgagaatga cacacatcgg
     3061 ctatcgcgga aatatcgctc atgccgccgt aataatctat cgaatttata aataccgcaa
     3121 gcgtccttga atttattttt gcctctatgc tttcgggggt gactgcggca ctgagatatt
     3181 cttctgnnnn nnnnnnccca cgagggggtg agcccgagca gaacacagga atttataaag
     3241 ctgcggtgag aatatctgcc cgccactata tggtccttac cgccgccgcg catttttgcg
     3301 agagtaagca tagtctgaat ggcaagcgta ctaccggagc aggagtagag cgtcctttta
     3361 cttccgaaca gccttgacgc gtttttctca ctgagcgcta ttatgccctt accgccgttt
     3421 gtgtcggtgt cgtaaaggct gtccgcaccg tttatttcgg taatgtcaag cgggttttcc
     3481 gtccccttgt gtcccggcat ataacagcgt atcgtatgac tttcgctgtt ttttacaaga
     3541 aaatcatgta tcggtgttgt catttcttcg attaaatttg aaatatccat caggtatcat
     3601 tccttatcat ttcgtttatc gctttattcc tgtaaacaag atcgtgtctt tcggtaaagc
     3661 cgagcttttc ccaaaattcg ttaccgctct cgttgtgtct gaaaaccacg agtgcgacct
     3721 tatttatccc gatatttttc atagcgtcca taaccgcatc gacaagggcg gtcgctatcc
     3781 ctcttttgcg gtactgcgga tttaccgcag tgtgataaat atagcctctg cgcccgtccg
     3841 agcctgccaa tataacgcct acgagcttgt cgccttcgtg agccgtaaag caggtgtccg
     3901 gattgcgctc aagaaacgca tttatgccct gttcgctgtc atcgagattg ttaagcccca
     3961 tgcctttgca tgacagccac agttcataaa catcgcggta atttccgggt gtcatttttg
     4021 ctattttcat atttcatttc ccctttgctt tttattttat cataaatttc tgagattttc
     4081 aatttttttg taataaaaaa ataaaaaaag gttgaattat ttgcggattt tcatnnnnnn
     4141 nnnntcagca gtagtacgaa atacgaaaaa caaaatcaaa gaatactttg taagccttta
     4201 cgggtttaca gaatttcaag aaagggagta gataaaatgc ccgcacctaa aagaagagga
     4261 agaccgagaa aggatgcgac cgccgctaag accttagcgg aaaacaaagc cgcggcaaag
     4321 aagccaacgg ctaagaaaac gtcagaaaag aagccgtcat ctgcggctaa gaaaacactt
     4381 aaacctcagg gaaatacggt gttcgcgctg gatatcggaa cgaggacggt tgtcggggtg
     4441 ctcggatata tggacggcga ggtgttccgt gtgactgacc ccgagagcat tccgcactta
     4501 aag
//
LOCUS       DAWAOG010000106         4494 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2487_length_4494_cov_2.240369, whole genome shotgun sequence.
ACCESSION   DAWAOG010000106 DAWAOG010000000
VERSION     DAWAOG010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2487_length_4494_cov_2.240369"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    731..740
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1787..1796
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          959 a         1208 c          970 g         1337 t
ORIGIN      
        1 gtatactgcg tgacgtgtct ttatgccctt gtcagcaagc ttctgacccg acagcttctc
       61 gatagtcctg tcaaggtagc tgtacttctt cgcaaggtca tatttctcct gcgtaagctc
      121 gccgtcctca agccacagct caagctctgc aaggttttta agcttggtta taaaccactt
      181 gtcaatagtg gttatatcat gtatctcctg aacgctgaaa cctcttctca gtgcctcgaa
      241 tacgcagaac gaacgatcaa catcgacatc gtggagcttt gctcttactt cttcgtctgt
      301 cagcttcttg aaagcctttt tacgcatcgt gtccatacca agctcgatag aacgtacagc
      361 cttcattata gcttcttcaa agctcgttcc tatagccatt atctcgccgg tagccttcat
      421 ctgagtaccg agcgttttct ttgcatatac gaacttatca aacggccact tcgggaactt
      481 tactacaaca tagtcaagcg caggctcaaa gcaggcgtag gtcttgcctg ttacctggtt
      541 tacgatctca tcgagcgaat atcctatcgc tatctttgta gcaacctttg ctataggata
      601 gcctgtagcc tttgatgcaa gcgccgatga acgtgaaact ctggggttta cctcgattac
      661 cgagtattca aagctgtcgg gctttaatgc aaactgacag ttacagccac cctcgacctt
      721 cagcgcctgg nnnnnnnnnn atgttcagcg ccgctgttct cagcatctga tattccttgt
      781 ctgcaagcgt aaccgcggga gcgataacga tactgtcgcc tgtgtgaacg ccgacagggt
      841 caaagttttc catcgagcag acagtgataa cattgccctt tgcgtcacgg ataacctcga
      901 actcgatctc tttccagccc gaaatgcact tctcaacaag tatctgcgtg ataggcgaaa
      961 gacgcagacc gtttgttgcg atctcgtgta attcgtcctt gctatatgca ataccaccgc
     1021 ctgtgccgcc gagcgtgaac gcaggacgta caattacggg atagtgtata acctcggcaa
     1081 aatccaaagc gtcctcaacg gtttcaacta cctttgaggg aatacacggc tcgcctatag
     1141 cctccatagt atccttgaac gcctgtctgt cctctgcctt gtgtatagtt tcggggttcg
     1201 caccgagcag ctttacaccg ttctcagcaa ggaagccttc ctcagcaagc tgcattgaaa
     1261 gggtaaggcc cgtctgaccg ccgagcgttg acaatatgct gtccggcttt tctatcttga
     1321 ttatcttctt tactacatca agcgtcagcg gctcgatata gatcttgtcc gccatatgct
     1381 tgtccgtcat gatggtagcg gggtttgagt ttataagtac gacctcgagt ccctcggctt
     1441 tcagcgcacg gcaagcctgt gttccggcat agtcaaattc agccgcctgt ccgattacga
     1501 taggtcctga acctataacg agtactttct ttatatcact tcttaacggc atttcttatt
     1561 ctcctccatc agactgataa atttatcgaa cagatacgct gtatcatgcg gacctccgca
     1621 agcttccggg tggaactgca ccgtgaaagc aggcgcattc gtgtacataa cgccctcgca
     1681 ggtcctgtcg ttgccgttga tatgacttac cttgcctgca ctttcgggaa ggctgtcgct
     1741 tacgaccgca taaccgtgat tctgcgatgt aatgaatgtt ctgtcgnnnn nnnnnngtcg
     1801 atagtaatat cggttacagg ctgatttccg cctctgtgac cgtatttcag cttgactgtg
     1861 gcagcaccgt ttgcaagtgc taagagctga tgaccaagac agataccgaa tgtaggtatc
     1921 ttgtgaggga taagttcctt cagcgtattt atggataccg tgttgtcctg cgggtcaccc
     1981 ggtccgtttg aaagcattat gccgtccgga gcaaatttca gtatttcctc cgcacttgtg
     2041 tcatagggga atactgtaac gtcacagccg cgctttacaa gctcacgtct tatattgaac
     2101 ttatagccgt agtccataag cgcaaccttg tatttgccgt tttcggcact gaacttctca
     2161 ggctgcttta cgcttacctt cggcacaaca ggacctacaa cataagccct tatcttttcg
     2221 agcgcctgcg ccctgaactc gtctgtcggc tcgcttgttg taatcatgcc gttcataaca
     2281 cctgtttcac ggattattct tgtaagtctt cttgtatcta ttccgaacag tccgatgata
     2341 ttatgctgct ttaagaagcc gtctatgtcg ccctcacagc ggaagttgga cggaacctcg
     2401 caatattcgc gcacgatata accgcttacc acgctcgttt ttgattcgta gtcctcatca
     2461 tttacgccgt agttaccgat aagagggaat gtctgcgtta cgatctgtcc gcagtagctg
     2521 gggtctgtca gcgtttcttc atagcctgtc attgccgttg taaaaactat ctcgcccccg
     2581 acctcgccct cacagccgaa gctcttgccc gtgaacaccg tgccgtctgc aagtatcaga
     2641 tatgcggtct ttgctttgtt agcctttaag tccatttaat tcgttccttt catatatttt
     2701 tcttttttgt tctatcactt aagggagctg tatctttccg acaaagccca ttatctcatc
     2761 tctcatacgc agtgcctctc tgaatgccgc acctgcaaaa tcctgctcgt cacagccttc
     2821 ttttttatat gctgtcatta ttcctcgcga gctgtttact atgccgccaa gaccgttcgc
     2881 atcgaatgcg gcggcaatat ccttggctgt tgcgccctgt gcgccgtagc cggggataag
     2941 gaagaatgtg tgcgggagct ttttacgaag ctcggcgatc tgttcgggat atgtcgcacc
     3001 tactaccgcg ccaacgcccg aatagccgta cttgccggga agctcctctc cccacttttc
     3061 gcacatattg cccattcttt cgtatacggg aataccgtct atcagcagat cctgaagctc
     3121 gccgcttgaa ggattagatg tctttaccag tacgaaaatg cccttatcgt tttccttgca
     3181 caccttgata agaggattga taccgtcgct tccgagatat ccgtttaccg tcagagcgtc
     3241 gcccataaaa ggctcgtatt cctcgtttcc tacctttacc ttgcccagat gcgctgcggc
     3301 atacgcttcc atagttgtgc ctatatcatt acgcttgccg tcagtgataa catacatacc
     3361 tctgcttctt gcatattcct gcgtcttgta cagcgttttc atgcctgccg taccgtacat
     3421 ttcataataa gccgcctggg gctttactgc gggaactacc tcgtggagag cgtctataag
     3481 gcatttgttg aattcaagta ttgcctttgc cgcacccttt agcgtttcac cgtacttgtt
     3541 gaaagccttg tcctttatgt actgaggtac atagtcgagc ttggggtcaa gacctgcgac
     3601 tgtagggttc tgcttttcgc agatcgcacg aagaagatta tccattgaca ttttcgtatc
     3661 catcctttat atttatttca agtatatcgg agcttacttg tcctcgtata ctatttttcc
     3721 gtttaccagt gtgtacttta ccctgccctt aagtgtcatt cccttgaagc aggtgttctt
     3781 tgactttgag tggagctttt cggggtctac cgtccagctt tcatcagcgt caaatattgc
     3841 aatgtcctca atatcgccta tgccgagcga gcctccctca attcccagta tctttctggg
     3901 attgacgcac ataagtctta ctatgtgcat aaggcttatc gtacccgtgt gataaagtcc
     3961 cgtcagcgtc gccgcaagcg atgtttccat tcccacaacg ccgttgggag ctttctcaaa
     4021 gtcagccttt tcctctgcgc tgtgcggagc atggtcggtt actatgcagt cgatagtgcc
     4081 gtctattata gcggcggtta tcgccataac atcgtcctcc tcgcgaagcg gaggattcat
     4141 tctgtagtcc gcatctctcg acaacagctt ttcgtctgtc atcataaagt agtgcggagc
     4201 ggtttcgcag gtgcacataa caccgatatg agtgttacct cttatcgtgt tcacactgcc
     4261 ctttgtcgaa acgtgcgcaa tgtgtatcgg catctgcgta tcctgtgcta tggcaagctc
     4321 tcttgccgtg attatatcct cgctcagcct gctcataccc tttacgccga gctgctgaga
     4381 tatctttccc ttgttgatta taccgccgtc tattatctca agatcctcgc agtgtgaaat
     4441 gaccttcagt cccgcatagt gcgcctttac catagcctgc gccatcatat tcgc
//
LOCUS       DAWAOG010000107         4491 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2489_length_4491_cov_2.559062, whole genome shotgun sequence.
ACCESSION   DAWAOG010000107 DAWAOG010000000
VERSION     DAWAOG010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2489_length_4491_cov_2.559062"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3143..3152
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1226 a          884 c         1099 g         1272 t
ORIGIN      
        1 cctgcacagg ttgcagcggc ggcggtatca acagcaatgt cgaaggtaca agtagtagct
       61 ctgacaatac cggcagcgat atcagcagca atatcgaaag tgcaagcaac agcaccgaca
      121 atactgacgg tgatatgccg gaagctttaa aacgtcagaa tgaaattaac tattataccg
      181 tcgacgaagt aaagaagatg atccgggaga atacaaggtt caatatatcc gaggattttg
      241 tgagccttgt gccaaagagc gtttcatata tctgttcttt tgacaaggcg gcagtaaaca
      301 gacaagctcc gacagaatat tatgatgact tcaaagagat gttcgcatat cttttcccaa
      361 atgaaaaatt caatgacgac tatttcttct actactatat tcccgatgaa gaaagcaata
      421 ggcaagaagg cgaatatccg ataaaaaatt tcaagaagta ttatgatgat gcggtttccg
      481 aaaaaaacgt aatatcatat tatttttatt ctccgtattc cggttttgtt gaaaaggttg
      541 gctcggatta tgctattttg gataataggg atagctcgga ggttaccgat cgcaatattt
      601 tccttgagtt cggtccgcct atcggaaact atctgtccaa tgtctgcaag ggtgttctgg
      661 ttgagcaata ctgcaaagat agcggcgaaa agaacaatca tttccttgaa aattgggacg
      721 ctgagatcaa agggcattat aaagtgatcg ggaggtacga ccgtgacagc accgagaagt
      781 tcagattgct ggacggtaaa gagataagcg ttgccgatgc ggtaactttt tttgaaaact
      841 atcttaaaaa tgctccatgc ccgaagtcac ccgtatatga tatgagggtt atgtctgtca
      901 ttgtacagca gtacgatcag gatagatatt tctattcttt tacgacaact ccgacatacg
      961 ggggaatacc ttttgactgg gatcgtttct atctcagcag tgacagtatg tatgaaacca
     1021 ctctgtgcaa cggcagtatg gttacgaccg atgatattga cagcttttac ggctacagaa
     1081 ggaccggttc gatcaccgat gaaaaggtaa cggataagct tctgccgttc gataaagcga
     1141 taagcaaaat agaacagtcg ctgacagagc aggtatcgtt tgaagtactc agtgccgacc
     1201 tggtatacac accgctcaaa tattacggat cgtatgatat tccgcttgag gagcagaaga
     1261 gtaaaacgac ccccgcatgg aaggtcgtta ttcagaacaa gaacaatgat ctcgtgtaca
     1321 cctgctatat agatgccgat accggagaga atttccgtta taataaggcg gcgttctata
     1381 cagcagagga agaggcagag ggctgatatg ttcagagcaa cgctttctta ccttaaacgc
     1441 atactcggag ataagtcgtt ttatatatgc ctgttgctga tatttgcaat atgctttacg
     1501 gcttctgccg actatgactt cgacaaaaat cagtccttgt cggtctggga tctgattatc
     1561 gacacacagc ggatatatac cggcaatatc agcttcagcg cctgtgtggt gtccgggctt
     1621 aagggcggca gctggctgaa tatgtttctg ccgttgcttt tgcctctgtg ctttgtaaat
     1681 gcgctgtgcg atgacaaaag ctcaggcttt acccgctatg aagttatacg cacaggctat
     1741 cgcagactta aaagtgcaaa gtatgtttcg gctgtgctcg caagcggcat atgctgtctt
     1801 atcgggtatt gcctttatgt gcttgttgtg ctgataaggt ttccgcatat agaagcttat
     1861 gatgccgaaa gactttctta cgtactgacc ggtattcaga tgagcatttt tgacagcgtg
     1921 gggatagcag gagtatatct tgtcaatatg ctcgatatgc ttatctacgg gatagtaatg
     1981 tcactattct cgttgatagt gacggcgtat actgaaaaca agtatcttat atgctgtatc
     2041 ccgtttatgg tcgcttattg cctgacacag ctcggctcat ggcttacagg acttgccatg
     2101 caggatccct gtaacacaga cgaagtgctc ttaacaatag gcaatactgc ttctccgatt
     2161 gcggcgagcg gtattgtttc gggagcgact gccgctcttg cgtgttatta cggcgtgctt
     2221 atactgcttt cgtttgcggt atatgtattt ttcggaaagg gcggtgcgga taaaggtgca
     2281 taaacagatc agcctttcaa agatgtggtc ggtcgcaagg tgcgaataca taaagtggat
     2341 aaccaatccc agaaatatag ttgtaatagt actgcttata tttataaaga cgcttttcat
     2401 cgatacgctt gcacagcgcg cagacaggta cggcagtccg atgaatgtgc ttgagccgtt
     2461 tatagcggtc ggcaactcgt gggtagcggt gcttttactg ccgtcggtgt tccttgtgct
     2521 tatgagcgat ttcccgaagt ttgacggcaa ctcgcttttc gttatctgca ggagcggaaa
     2581 gctgaactgg ttgtacggtc agatgttatt tgctgttatg gcggtaataa gctatatagc
     2641 tgttgtattt cttgcagttg tgggaatgtc tgtcgggaac acggtgctcg gttcatcgtg
     2701 gagtgaagct gcaagatttt atgcggcaaa gtttccagat gaaatgaata ctttcgctac
     2761 tctgctgttg cctgaaaatc tgtataatca gcttgacccc atatgtacta ttgtacacac
     2821 gttggtactc atcatattat acctttttct tttgtcgctt gtgctcctta tttgcaggat
     2881 atcatcaggt aaaatgacag gtatgttcat atgtaccgcg gtcatagccc tcggagtggc
     2941 tacctgcgca ctgcgcagtg atctgatgtg gctgtttcct atgggtaatt ccataatatg
     3001 gatgcactac acggagataa tccgtgaacc gataaagccg atatggtatt cctacgttta
     3061 tttcgccgtt atggctctgt tgcttatatt tatgagcaca ttgagagcaa ggcgattgag
     3121 cgtatcactt cttccggagg aannnnnnnn nnacgataaa caaaacggag atactccgtg
     3181 atataacaat atcttttgat aaagggaaaa tacacgggct tatagggcgc aacggaagcg
     3241 gcaagactat gctgatgaaa tgtatatgcg gatttgttcg tgcaacctca ggcaaggtaa
     3301 cggtagcggg caaggagata ggcaaggact gtgattttcc cgaaagtacg ggagttataa
     3361 tagaaacgcc cggctttatc ccgtattatt cagggtataa gaatctgaag attcttgccg
     3421 acctcggcgc aaaagcagac aaagaggata tatgcaatgc tatgcgcacg gtgggtcttt
     3481 cccccgagct taaacgccat gtgaaaaaat attcgctcgg tatgcgtcag aggctaggac
     3541 ttgcacaggc tataatggaa gacccagacc tgcttatcct tgacgagccg atgaatgggc
     3601 ttgacaagga cggcgttgcg gatatgcgcc gctatctgct tgatctcaag gcaaggggca
     3661 agactataat cattgcctcg cacagtgcgg aagatattga ggtgctgtgc gacaccgtat
     3721 gtgaaatgga taagggcagg ttgagcgtga taagataata accgtccgaa caggataacc
     3781 gccgcagatt tttaaaacct gcggcggttt tttgcgttgt attgaaaatt tagggcaaaa
     3841 gttgcaaaaa aattaggtag gagatataaa atcgtccttg cttttactta tatcgtaaat
     3901 tgaataaaag acaccgccgt ggagcagtaa ctccgcggcg gtatctttat ttgatcatat
     3961 ctataacaga actgagtgta tcttttacgg cttgcttttc gctttcggaa aggcgtgtta
     4021 ttttatccga aagtatcccg ataggcgaaa gggagctgtc agatgtcaga gggtggcggc
     4081 ttgaggaaat tcctgcgata taatcaaggc tgacattata atattttgca agcaggactg
     4141 cgcgctcaag cggtatttcc gctttacccg attcgtattt tccgtagtgc tgtgcagatg
     4201 tgccgagata gtcggctatc tcctgctgtg tcttgtccgc gtcctcgcgc agatcgcgta
     4261 atctctgata atgtgccata ctcacccctc ctgtacagat tttatcatat cagattgaaa
     4321 attgttgaca aaagtgtctt aaaagattat aatataatta taatcagtct gattagacac
     4381 atttatgtgt cggaatgagt taaataggag gtttttatga aaatcaaaac aatgtttaaa
     4441 gcggtattgt cgcttgcggt ctgcctaatg gcaatgttgg taatttcagt t
//
LOCUS       DAWAOG010000108         4490 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2490_length_4490_cov_2.124239, whole genome shotgun sequence.
ACCESSION   DAWAOG010000108 DAWAOG010000000
VERSION     DAWAOG010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2490_length_4490_cov_2.124239"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    864..873
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1083 a         1062 c         1124 g         1211 t
ORIGIN      
        1 ctcttaccct tgccaagctc tgtctttccg tagtcgcttc cgcttgaaat gacctcgccg
       61 acagtttcgt tgaaccatac ggggcttata aagaagttta aaaatccagg tcccgcaacc
      121 tcgatctttt caaatacagt gccgtcaagt attgcggcat cggctatcat ctgacctatc
      181 gctctggggt tattcttaag cgctttggcg cttgccattg cggcattgca tgagaagtcg
      241 ccgtgtgata catcggcggg acgttctaca ttgaatgcgg gcaggggaac tgcctctatc
      301 ttgccctccg caaccagtct gcccagtgcg ttcattaccg tttcctttac ctgctgttta
      361 gcaagctcta tcatattttt catcagttac actccttgac gtttataaac atttcatttt
      421 ccgacatata gcttgaattt atgttgagcg tgtatttgag gtagatatcc ccgcctgttt
      481 cttcaagctc atttcttatt tcgtccgtgc atacgcctat cataaagtcg ccgaattctg
      541 tcccgtagtg gcagtggtgc ttctttcccc gctcgataaa cagtgagctt attgcctttc
      601 cgcttcttgt catagttatc atatcatcgg tcacttcaag cgttaccgag cttcccgcaa
      661 aaccggtagc ttcgctttca tcgtaataaa gacgaaaacc gttctcggta cgctccatag
      721 cgccgaatgt gaacaactcg gtagaatcct tatcctgatc taccgtttgt gtgcttttta
      781 tcgtaataca tacgttcttt ttcatttatt atccgttgtt cctttcaaag cctcaatcga
      841 ggtcaactac agcacctatg agcnnnnnnn nnnaccgggg attcctctta cgcgtcttac
      901 atagacctct ccgtcaacgc cgagtaccat gtcaactctt gcgaagcact tgcaggaaag
      961 cgcatcatat gcggctcttg cggtctgctt tatcttgttt tgggtaagac cgtcaagttt
     1021 agcgggaact atcagctcgg aagttgatgc gatatactcg gaaagcttat cgatattgtc
     1081 atccgttctt acaagctcgc ctatggctac acgttcgtca tggtgctttg cgccgagtac
     1141 cgcgcaggca agctctcttc ccttaacggc gctctcggct atgatataag ggtggtgaga
     1201 aaagcttgtt tttacagcct cctcaagctc cttttcgttt tccgctctgc aagcgccgac
     1261 tgatgttgaa cagcttgtcg gtgcagtata tatagggaaa ctcagtctat cggttatctt
     1321 gcttatagcc ttcgccatat cgttcatttc actgcggtga agcgtgacgt tttcaattac
     1381 ctttatgccg ctgtcggaca gcaccagatc cataatacgt ctgtcgttgc acagtgccgc
     1441 cgccgcaaaa tcgttgccga taataggcag tcccgcaagc ttgcacagtc cctgtactct
     1501 gccgtcctct ccgtactttc cgtgaagcac ggggaataca gcgtcaatac ggtgtatggt
     1561 gctctcaccg tcggagatta tctttattac gcctttgttt gtcatatccg ggcttattat
     1621 gcatgaacag caatcgctgt cctgctccca gctgccgtcc tttatattct catagcttcc
     1681 ggggtaaaac agccatctgc cggctctcgt aatgccaatt gcgagcggct ctatctcctt
     1741 tggcatggcg ctcaacacgc tgtatgcggt gtggagcgag gcggcatggt cagcggaagc
     1801 accgccgaac agtacggcta ttcttttttt cgccataggt tttcctcttc tttcgttctt
     1861 tgacttttat acaataataa gataagtata tcacattcgg cggtcaagtt caagtatcag
     1921 cgggtaaatt atatgaatag tatcaggggc gtttgtcgtc tttgtattct tttggggtga
     1981 caccgacagc tttgctgaaa gcacggcaga aatggctctg tgacgagaac ccgagcagtg
     2041 atgcgatatc ggcgatactg tgatctccgt cccgcagcat tcctttggcg gcgttgatgc
     2101 gtacggcgct gatgtactcg tgtattccta tgccgatctc cgccttgaac aggcggtgca
     2161 ggtgggagcg gtcgtagccg atggtttcgg cgatagactt tgctgtaagg ttctttccgg
     2221 tgttctcgga tatcatcttg atagccttgc ggacgggcac ggagagccgt ttcgtgcgcc
     2281 ttagcctgcg cattcttccg gcgtaatcca tcatcatatc atcattcaga tccatgacct
     2341 gttttacagt cgtacacttg tcggctcgtt gaatgtacag gtcgctcaga gtgtaggcgg
     2401 tttcctcgga catacctgcg gcaaggcagg cgcgggcgac aagcgcggcg cataccacca
     2461 ggtggtatat ctggttgcgg agcggatcgt cggatagctg tccttttccg ctgtcgggat
     2521 ctgcatcgta tctgagctta ttttcgagta tcttttcgac ctcgcccgag gctacaagcg
     2581 caaagacggt gttttcattt tcgacaggct catgaaaaaa gtcctgtgcc tgctgtacat
     2641 aaaggagctt atcgggctct gtattttcgt ctgtaccggt ggttggtacc atggtacatt
     2701 ttgcggcgtt ttcgggtgta tccgacctga ttttctgctc attggcagat actgtatgtt
     2761 tatctgtcat atttaacctt tcttgtatgt ttttttaacg ttgaaaacat tatacaacat
     2821 ttatgcaaaa atagcaacat ttttaatata cctgaaattt ggtttgtgct aagataaagt
     2881 tgtaatcaaa aatgtaagat gggtgaatag caccctgacg gaaaaggagc taatatgaaa
     2941 gcaagggcac tcaaggcgat aatagcggcg gtactggctg tatcgatgct ggcaggctgc
     3001 gcaggcaata catcatcggg cggctcatcg ccgatatcgt cagatacggt atcatctgac
     3061 gtgacaacat caggcgcgac atcatctgag gcgggatcgt cagatacggt atcatcatcg
     3121 gcaaactcag ccgatgaaag cacatctcag ggcgtttctg cggaggacag cagcgaaagc
     3181 agtactgaca gttctgcgca gagtagtgag cagagcacaa cgcagtcggg tgaagaggag
     3241 agcagtaacg acacctcaga gcctgccggg gagagtgagc ccggagtgat gcgcgacatt
     3301 acttctcagc agcttgtcga tgatatgaca ttcggctgga acctcggcaa cacgcttgac
     3361 gtttgccagg ctgaccgtga cggtgacggc aaggtaaacg agcacgttga agagggcgaa
     3421 aaggttgacg aaacgctgtg gggcaaccct aaggctacat atgaactgtt cgctcagctt
     3481 aaaagcgatg gaataaatgc ggtgagaata cctgtgacgt ggcgtgacca tatggacgct
     3541 gacggaaaca tcgacaagga gtggatggac agggtgcagc aggttgtcga ctatgcgtac
     3601 tcgcaggaaa tgtatgttat aatcaacgtt catcatgacg gcggcggtga tccgaagttc
     3661 ggcgcgtgga taatcgagga gtcgcagaag gactatgacg cattcctgaa aaagtataaa
     3721 aatgtctggg aacagatcgc cgagaggttc aagaattact ccgactatct gatattcgag
     3781 tcgatgaacg aggtcggatt tgacaacctt tacaacaaga acaaggcgga cgcatacgac
     3841 ctgataaaca agatcaaccg ggactttgtc gacataatcc gtgcaacagg cggaaacaac
     3901 gcaaagcgtc atctgcttat agcgggatat tacaccgaca tagagcgcac ctgcgattct
     3961 ctcttcaaga tgcccgaaga ccccgaaaac agatgtattg tttcggtgca ctactacaca
     4021 ccgtgggact tctgcacctg tgacatcaag cacacatggg gcacaaagaa ggaagtaagg
     4081 cagatggaaa cccttatcgg caagatgaag acgaactttg tcgacaaggg aatacctgta
     4141 ataatcggcg aatatgcggc aagcggaagc gacctttctt cctgcatttt ctttatcgaa
     4201 aagctgaaca agctgtgcag tgattacggc attgcgacat tcatctggga caacggcgga
     4261 cagataaaca gaaccaccta tcagtggaga acaccgcagt atcttgaggt acttaagcgc
     4321 gcgacaagcg gagaggacta tgaggtagtg aagagctgag aggcggcgtg gaacagatgt
     4381 acatgacgga aagttaatat ttatgaccct gctgtttcgg gaacggaacg gcggggtttt
     4441 tgtgtgtaat gaataatgaa taatgaataa tgaatgatga ataattgtgg
//
LOCUS       DAWAOG010000109         4490 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2491_length_4490_cov_2.101466, whole genome shotgun sequence.
ACCESSION   DAWAOG010000109 DAWAOG010000000
VERSION     DAWAOG010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2491_length_4490_cov_2.101466"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3676..3685
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1440 a         1027 c          994 g         1019 t
ORIGIN      
        1 cttgataacg gtgaatatat ccacggagat tatctgagtg agagctgact ctgatataac
       61 gcatttcccg gcatttgatt tctgtacgca acactgccgt tatgcattta aagcgcacgt
      121 tttttcacat atatcaagga gctaaattga attatatcat aaacagaaaa attctctgtg
      181 tttttcttgc ttttacagtg attttgccgt tttctctcac ctgctttgcc gaaaacggca
      241 gcagctcttc cgatgacact gcaaaaaagc ctgataagtg ctatgaactt atcaccggag
      301 gtaatgagga atattactgc aatatcacaa taaacagccg ttccgtactg atagacggct
      361 gttacaaaaa cgaccccgtg acgaacatca ccttacagaa ctgcggcact gtttcctcca
      421 ctcttaaagt aaacgccgac ggaacgttct catcggtact taacccctct gagccaaccg
      481 gaagctccga ccgcatagtg atcacgctga agtccggcgc aagactgagt tatcctataa
      541 tgtacgatga taaccgttac ttccccgaca acaaactgag cgtacaaaat ctttctgtgc
      601 tggaaaaaac agtcccgacc tccgcaaaat catgggcagg ctatgttacc gatgagctga
      661 cggaagacag cgtaaagcag acgcttgacg aagtcgcgta tcttgcagat tacattgcag
      721 gcgacataaa ggataactat aaaaaactgg aagctagttc aaagtgggta tccgacaaca
      781 tctactatga ccgcgatgca cgcgaaagct cggtaacgca atccgagatc tgtataaaaa
      841 acgttcttaa agaacgtaaa accgtctgtg tcggctattc agcgcttttc agtgcgctgt
      901 gtgaggcaca gggaatttat gttgtgaacg ttaaagggac agttacatca gacactgtta
      961 aatatgacga tctcgctgac ggtcctgtca atcacgagtg gtgcgccgca atgatagacg
     1021 acaaatggat atgggtcgac tgcgtgtggg acagcaatct gaaatttgaa aacaacgagt
     1081 ttaaatctat tagtgcgaac acacagatgt attttgacat ttcaccgctc gcgctctctt
     1141 tcgatcactg cgcatatctt gccgaaaaac gatattattt ccgtgccggc gaatatttca
     1201 agcagtcgga aacaaccgtt caaagcacag ccgaaaccgt agcgtcaaat acagacagca
     1261 ctgcaagcag tacttcagcc tcaaccgccg tacaatcgac tgcttcaccg gacacaagca
     1321 agccgcagac ctacactgcc ccggcagatt atactccggt aacaagcgat attcaggcac
     1381 aggaagagcc cgtaagcaac aatcttatct ggatagctgt gctttctgtt gcggtagcag
     1441 tcgggcttgt ggtagacatt atcaatatta aatggataag aaaaaataag aaatagcccc
     1501 gaaaggaact ccgttatgaa aataaaactc ctcgccgcgg ctcttataac cgcactctgc
     1561 ctatccggct gttcatcggc ggcgacctca tcgcagtcct ctgccgaagg ctcgactccg
     1621 acaacctcat cagcttcgac agctgtaagc accgacaaca cctcatccga gcctgaaaac
     1681 agctcgaaag atgaaaccac agctcccgag agcgcagaaa catctgctcc cgaaaccgta
     1741 gctgaaacca cagctcccga gagcacagaa acatctgcac ccgaaacctc agctgaaacg
     1801 accgctctga cagacgaaac tgtctcctcc gcacccgaag acggaaatac ggtaaagcgt
     1861 atcgattatg agcttgaccc gtcaaagccg acaatagcga taacatttga tgacggtccg
     1921 aacaccacca caactatgga aatacttgac gtacttgaaa aatatcaggt aagagcatct
     1981 ttcttcctta tcggaacgaa tataaacgat gaatccgcaa aatcagtaaa gcgtgcttat
     2041 gatctcggtt gcgacattga aaaccattca aagacacata gctacatgga taagatgacc
     2101 gctgatgaga taaaggacga gatcgcatat gtaaacgaca aggtaaaaga gatcacaggt
     2161 acgaccccaa agttcttccg tccgccgtac atagctgtaa acaatacaat gtatgacaac
     2221 attgatatga cttttatcag cggatacggt tgcaacgact gggaggacag agtgacagca
     2281 gagtacagag ctaagtatct tgagaaaaaa gccgcagacg gtgtgatctt cctgctccac
     2341 gatgccgagg gcaattcaaa gactgtcgaa gcactcgaca aggcgatccc gatacttctt
     2401 gaaaaaggct tccagtttgc aactataagt gagctgtttg agctcaaggg cgtagagatc
     2461 agcggaacgg acaccaatat ctactccgag cttaacgcac agtaagacat cggtaaggcg
     2521 aagccttatt gacatacagg aaaacgtaat atgaaaggaa tttaaaccaa tggtagttat
     2581 cgaaatggaa aacggaaaaa agataaagat agagctttat cccgaaatcg cgcctatcac
     2641 ctgcgcaaac tttgaaaagc tcgttaagga aggcttttat gacggactta tctttcaccg
     2701 tgtaatcagc ggctttatga tccagggcgg tgaccccgag ggcacaggca tgggcggcgc
     2761 aaaggaaaac atcaagggcg aatttgctca gaacggcgta aagaacgacc tgaagcacac
     2821 aagaggtgtt atctcaatgg cgcgttcgat gatgccggac agcgcaagct cacagttctt
     2881 tataatgcac aaggacgctc ctcatctgga cggcggttat gccgctttcg gcaaggtagt
     2941 tgacggtatg gacgcagttg acgaaattgc agataccgag gttgactgga acgacaaacc
     3001 cacaactcct caggttatga agagagttta tatcgaagac taacagcgtt taaaccacgc
     3061 aaggtttaca gcacaggctt ctgtattatg cagaggcctg tatttattat aaaaagtttt
     3121 ttataaattt tctcaaaagc gtgaccttta taaaaatatc ctgtctttat aggtgaacag
     3181 gctgtttaaa aaaataaaag ggagtgagaa atacggaaga cagcaggatt atagaattat
     3241 tctttgcaag agatgaaaaa gcgatatcag aaacccacag caaatacggc agatactgtt
     3301 actctatcgc ctacaacata cttgccgtga atgaggactg cgaggagtgc gtaaacgata
     3361 cgctgatgaa ggcgtggaat gccattccgc cgcagaagcc aaaaaagctg tcggcatttc
     3421 tcggcaggat aacacgcaac ctttcgctga acagattctt tgaaaaaaca gcagataaaa
     3481 gaggctcggg tcagacagcc gccgttcttg atgagctggc tgaatgtgtg tcgtccggtg
     3541 ttactaccga ggatgtttcg gacaacaaca cattaagacg tgcactcaac gtctttctct
     3601 gctcacttga acccgaaaag cgcattatct ttatgcagag atactggtat atgatgccgg
     3661 taaaggcgat agccgnnnnn nnnnnatagc cgccgaacat tcatctaccg aaagccgcat
     3721 aaagatgatg cttttcagaa cccgaaacga gctaaaagaa catcttataa aggagggtat
     3781 aacgctatga aagaaaaacg cttactgaat gaactcggca acgtaaacga tgatttcatc
     3841 gaacaggcaa agccgaaaac aaaaaacaac agaaaaaagc tgtacgctgt tatcggctcg
     3901 gtggcgactg cggcggcagt gtttgcggta gtattcgctc tcagcaatca cattcccggc
     3961 aatgacacca ttccaaccgg ctcatcaaac gctgtagaag aaatatcttc caacgaaata
     4021 gtcgaaacac cgcttacatt tgaagaagtg aaaatgccgt cgctcagcac tcattcgagc
     4081 ggaggcggag atgaactcgg caattttcac ggaatttccg aagccaaatt ttgcttgata
     4141 ccgcttgaat tagatcgcct gatagaagaa agctgtccgg aaggcacatt agaggattat
     4201 tataagtggt tggaagaata cgaaaaacag cattataatt tagacaaaat accacctata
     4261 aacattgaag aagtcccatt tttccctaac atagtcagaa aatattcatt agaccatgat
     4321 acaatagtcg aaatgctaaa aaagcgtaat gacgtttata aacaacaggc ggcagacaac
     4381 aatttaggcg atgaatactc tgagtacatt tacacagatg aagagataga agctatagcc
     4441 tccggcgatg tacagaaatg ctatgattta gtcacattca cgaaatcgat
//
LOCUS       DAWAOG010000110         4489 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2492_length_4489_cov_2.086152, whole genome shotgun sequence.
ACCESSION   DAWAOG010000110 DAWAOG010000000
VERSION     DAWAOG010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2492_length_4489_cov_2.086152"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2716..2725
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4189..4212
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1405 a          882 c         1217 g          951 t
ORIGIN      
        1 atggcaaatg acccggcgat tatccttgcc gacgagccga caggtgcgct tgactacaat
       61 accggtaaga tgatactgaa gcttttgcag gatacctgcc gcagcgacaa aatgactgta
      121 atacttgtga cacataatac ggcgatatgc cccatggctg acagagttat acacatgaag
      181 agcagtaagg tcagcgagat agtcgtaaac gagcatccga cacccgttga agaaatagag
      241 tggtaagcgg ggaaagggaa cggatatgaa aaatgcaatg ctgaaaaatg catttgtcga
      301 gatgagaaag acgaaaagca gatttttttc catattcggc ataatagcga taggaacggg
      361 cttttttgcg ggactgcttg ccagcgcgcc tgccatgaag atatcctccg atcattacta
      421 tgaccgcacg tcactgcttg actacagact ggtttcgaca tacggctttg atgaaaacga
      481 tataaaggcg cttgaggacg ggcttcccga tgtgaatatt tatcccggat attacacgga
      541 cgcttatgtc aataccgact cgggtgagaa aaaggtggca agggtatatt ctgttgacac
      601 gatagggaaa aataatccat acagttcgtt gaatataacc gagggcaggt tgccgcaggc
      661 tgagaatgaa tgccttatcg acaacggcaa gctgataaag aattataagg tcggtgataa
      721 gctgacgctc ggcggagatg aaaataccga tattgacgaa acgctttcgg tcaaggagtt
      781 tacaatagtc ggcaggtttg agtcgcctat gtatatatcg gatgccgagc gcggcaacac
      841 gaccattgga aacggttcgg tctttatggt ttgctatgtg ccgcagagct gcttcaagac
      901 cgaggtctac actcaggttg tgataacctc cgacaagctt aaggagtaca actgctacag
      961 cgatgaatat gaaaatgcgc gcgatgagct cggcgagcgc attcagcaga tagcggacag
     1021 ccgggaacag ggcagatatg acgagataac cgaagaggca cagagcaaga tcaatgacgg
     1081 tcagaaagag cttgatgaaa agaaagccga tgcgtacaag gaaatagatg ataacgagaa
     1141 gaagctcagc gatgccaaaa aggagcttga cgatgcgaaa gttcagcttg ctgacggcaa
     1201 agcacagctt gcagacagta aaaagcagct tgatgacgcc aaaaagcaac tcgatgaaaa
     1261 tgcgcctgta cttgcggaca gtaaaaaaca gctcgacgac agcaaaaaac agcttgacga
     1321 cagcaagaag cttcttgatg acactaaagc acagttagac agctcaaaat ctcagctcga
     1381 cagctcggca caacagcttg acgatacaaa aacacagctt gacagcgcaa aaaatcagct
     1441 tgacagtact tcgtcacagc tcgaccgtgc taaatcacag cttgacagcg ccaaggctca
     1501 gctcgatgag agcaaggcaa cgcttgacgc tacagacaaa cagcttagcg acagtcagaa
     1561 ggacattgat aattcgagag cgcagataaa cactgcggtc gcggccggta tgatgacaag
     1621 tgatgaagcc gctgtaaagc ttgcgcagct tgatgctgca caggaacagg taaacgcggg
     1681 cagagcacag tatgagagcg gacttgctca gtataatgcg ggacttagtg catatgaaaa
     1741 gtcaaaggca gagtatgacg cgggttatac gcagtacagc acggcaaagg ctcagtacga
     1801 aagcggactt gcacggtata atgacggtaa ggcgcagtat gagagcggac ttgcccggta
     1861 caatgacggt aaaaagcagt acgaagaggg tcttgtgcag tataacgagg gcaaaaagca
     1921 atacgaggat gggcttgcga aatacgaaaa gggaaaagcc gaatacgaca acggcaggaa
     1981 gcagtatgag gatgggcttg caaaatacaa tgacggcgca gcggagtacg aaaggaatta
     2041 tgcctcatat gaagacggtc ttaaggaata taacgacggt gtaaaaaagc tcagtgacgc
     2101 aaagtcggag gctgataaaa aatttgcgga tgcgcagaaa gagatagatg ataacaaaaa
     2161 gaagcttgat gatatcgaaa agccggaatg gtatcttttt acccgtgagg accaccccgg
     2221 atacagcgaa tacgggcaga acgcagacag aataagtaat atcgcaatgg tattcccgct
     2281 gttctttgtt atggtagcgg cgctcatatg ccttaccact atgacaagaa tggtcgaaga
     2341 acagcgcacg cagatcggaa cgatcaaggc gctgggctat aaaaacggtg ctataatatt
     2401 caagtatatg ttctatgcgc tgactgccgc cattgccggt gcggtgatag gtatgcttgt
     2461 cggtatgaag atctttcccg ctatcatcat cactgcttac ggaatgatgt atgctatacc
     2521 ggacattctt ctgccgtatg attttgtcct tattgcaggc acgacagcgg cggcggtact
     2581 tcttacggct gtaactgtat atttttcgtg cagcagtata ctccacgact gcccggcggc
     2641 acttatgcga ccgaaagctc cgaaaagcgg aaaaaagata ctgcttgaac gcatggggtt
     2701 tgtatggaac aggctnnnnn nnnnnggctg tacagcgctc gtgcttacgg gatttggaat
     2761 ttatgacagc gtgaacgata tcgtggaaaa gcaattcggc gagataagca actacaccgg
     2821 tatagtcgct tacggcgata agcagacaga tgaacagatc gcagaaatag ccgataagct
     2881 tgccggatac agctgtaagg gcgataagat ctatcaaaag cagataaccg tagcaagcgg
     2941 caaaaacagc acagatgctt atatattcgg tgcaaaggat aatgatacga tcacgcagtt
     3001 tgtaactgtc aaggacagac gtacaggaga gcactacacg gtgaccgatg acggcgttat
     3061 aatcaatgaa aagcttgcta cactgctcgg cggaataaaa aaaggtgacg agatcacatt
     3121 gtcactgtcg gagaccaagc gtgtaaacgc aaaggtaacg gagatatgtg aaaactatgc
     3181 gcatcattat gtttatataa cggaaaatct ttataagaag ctcagcggtg cagaagacct
     3241 cccttataat tgctatttct tcaactcgga gcaggacgat gatatgccgg aaaacgacag
     3301 ggataagctg gcggcggatc tgatgaagac tgacggcgtt atgggagtca gcttcaagac
     3361 cggagttaaa gcgacatttg caacaatgtt gaaatcgttg ctttttgtaa tagtggtact
     3421 catagtaagc gcgggtgcat tggcgtttat agtactgtat aaccttacca atataaatat
     3481 aaacgagcgt atccgcgaaa tagcatcgct caaggtgctg ggcttctacg ataaggaagt
     3541 ttcgatgtat gtgttcaggg aaacagtgat acttacgctg ataggtacgc ttgcaggtat
     3601 ggtgctcggc agatttctgg tcgactttgt tgtatatggt aatgttcggt agggaagtgc
     3661 atcctatgag ctttgcgctt tcagctgtca ttacaattat atttgcttta acggtaatgc
     3721 tgtttatgca taggcggctg atgaaggtcg atatggtaga ggcactgaaa agtgttgaat
     3781 gatattgggg cttaagcccg gaggttgatg gtatatgaaa ctgggaaaag caatagttgc
     3841 ggcggtcggt gcggctgcgg cggtcggtgc ggcggtatac gcaaaaagac gattggacga
     3901 aacgcagaca tcgcttaaaa aacataaaag cgttacaata tggaaaagta acggaaatgt
     3961 aacacacaaa gaaataatac gagagcctgt gagcgaagag caacagacag atatactcaa
     4021 aagcaggata aataccgctt cttgcgagtg caaagaattg tcacggtgtg ataacggcga
     4081 agagcaggag ataacgcctg tcgattttat attcaccgag gcggaggaaa gtgaaaatac
     4141 aggcgtaagc tttgaacgtg tatttgatca ttttaacaag cttgaagann nnnnnnnnnn
     4201 nnnnnnnnnn nntgcaccgt gaccgaaaga acggaagatg acggcgtttc cgccactgat
     4261 gaagtggtcg cagacagtac gggcctgaag gcggacgaag atgcgctgca ggctgccgct
     4321 atcgcggctg ttatgactat gcgggaagaa aacagtgagc cgtttcagga gatacttgcc
     4381 acagaggctt ttacatcgga gcctgtcgaa gaggcggtaa aagcatttga ggagatagag
     4441 gagcaggaaa aatcaagcga agagcagccg gaaaaagcag aagaaaagg
//
LOCUS       DAWAOG010000111         4464 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2513_length_4464_cov_2.942617, whole genome shotgun sequence.
ACCESSION   DAWAOG010000111 DAWAOG010000000
VERSION     DAWAOG010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2513_length_4464_cov_2.942617"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1259 a          983 c         1117 g         1105 t
ORIGIN      
        1 aagtaataca gccgtccgcc gataagcgta agcagtgtcg ctccgctgtc ggcaagcgca
       61 ccgagagagc tctgcacgga ctgcacgatc cctcccgaga agttgtaaat atcttttgcg
      121 agataagtga atatgatatc cgccgcgaac aaaagaataa aggataccgc atatacactg
      181 aaatagaatg tctttttgcc gcctgtaagc tttgtataca agctgaacag gcacactgca
      241 ataaatattc ctatacaagc gggtgacaga aaataagcgc ctgcggtaag caaccccgca
      301 ataaccgatg caaagaatat tcgtaccttt ttaccgcttt ccttctctct cctgaatatt
      361 acaagcagaa ggagcatcac aaatacagcg gcaagcggtt cggacagcag atagtgcgaa
      421 tagatgagca ccgacgggaa aatgccgcac agcgcggtta ttataagtct ttgccacagc
      481 ttttccacac cgagcctgcg tgacaagcgg aatgccgcaa gcggtatcac tgcatatgcc
      541 gcagaattta tcagcataaa caatttatac tggagcacag gatcggggca aagcatggtc
      601 ggcagatacg gaatgccgcg gagaaatccg ctgatactgc cgacagacgg cattacacct
      661 gtccagtccc tgcctaaaaa tgcggcggcg gcggcagcac cggtaaattc ctcctcaaga
      721 gaggctgtct gcataaacat tccgagcgca gagccgagta cgaagaatac aaaaaacaca
      781 aggagcgaca tacctttttc gccctttttg ccgtaaaaag tattgagttt tcccaagctg
      841 ccgccactcc tttcgtcaaa tccgagtatc gttgttgtca gttcataagt gcgtttacga
      901 gagtatttcc gatgccgagc atgttgttga tgagctggaa cacaaaaatt atagctatcc
      961 ccgagcaaag cgagaatgcc acagcataga aaaggctcgg tcttcccgcc gcttcaatcc
     1021 ttgcaagtat acggtcatag ttctcatctg agtagtgatg ttttattgca tttatccttt
     1081 ttatgcagga cttgaagtaa aagtagttga agaacatcag cacaaatatg tcaatagcaa
     1141 gcgaaagcgc agttgcgatt atataagcgg tcataagcat attgtcgctt atcccgagct
     1201 gttcggcaaa atgactgagc agtatcttcg cttccgtagt aaggaacaga agtgtcatca
     1261 taactactcc gaacaggttc atcttgcggt aaaagcagtg catcgggaac atcagaaaag
     1321 cggcaaaatt cagcttgagc gacttgttgt ttttccgtat atccttgaac accggcagat
     1381 aatagtatcc gttctttccg atgaacttaa gaagctcctg agcggtcacg ccgtctatag
     1441 cgtcaaagtc tttctgctgc tcgagcagct tcttgtccat tatttcgcgg tattcgtcat
     1501 agctgtcaac gtgtattatc tcaaagccgt catttgtatt ttcatgcttt tgattttcgg
     1561 cactctctat gttttccagg tgctcgcgaa gctcctgatt ttcacctttc cactcgtagc
     1621 tgccatgctc ggcggagtgt acacaagcac ctattctttc ccagcactcg cggtggtacg
     1681 gtgtgccgca ttcggggcag acgactatat ctgcatcgtc aaagaatttc atgccgcagg
     1741 cggcgcattt ctcattttta aactcttcgt gcattttata tccttaacct gattttgttt
     1801 agtgactata tttaatatat tataccacgg tttaaacacg ccgtcaaggt gtgttttctc
     1861 tgatgtgtgc gacagcacac cgtgtataac gcgataaaat cgatttatga cgatttttag
     1921 tttgggctgt tattgcgcaa gacaggatat tacggtgcgg aggacccgga gcgtttgtaa
     1981 aatctgcttg tataaacgga ctcaggggtg tataatatca gcaaggacga tctaaaggag
     2041 tggttgtatg aaaaaaagga tactatggat attgacgggc ggaacatttt cgtgtgtcgg
     2101 cacggacaga ggtcttatgc caagatccgg cgatgaacag gcggaaaaga tgctgtcgtt
     2161 gatgccggac atcattgaaa attacgatat tatgccgctt gtgcttatga atatcgacag
     2221 tagcgatatg acggtaagcc atcacagaat gatagccgat gcgatagatg cgaacattga
     2281 caagtatgat gggatagtta tgacccacgg cactgacact atggcgtata ctgccgccgc
     2341 actgtcggtc atgctccaaa atcctccgat accgatcgtg cttacgggtt cgcagcgccc
     2401 tttctttgat gagaacagcg acgcgccgaa gaattttgcc gatgcttttg aggtttcgga
     2461 taaggctgtg accggtggta tagttgtcgt tttcggcgga cgtatcatga gagggtgcga
     2521 ctgtgtaaag gtcgacacgg tcgccgatga cgcttttcgt tcggcgaccg ggaacgaggg
     2581 aacgctcaca aacgtcacag cggcttttcc cggcaacgaa gatctgccgg aggatcacgg
     2641 cgggtacagt tacgacagtt cgctttgtaa tgcggacgga aaggtcgcgt tgcttgatat
     2701 gcacccgtct ttcgatccgg atatagtagg gctgttatgc gataacggaa taaaagggtt
     2761 tgttatccgc tgttacggag cgggcggaat acccgaaaga gcgagggcgg cgtttggcaa
     2821 ggcgattgca aaaggagcaa aggcaattgc tgtgacccag tgcctgcacg gtggtacaaa
     2881 ccttggtata tacgctgtcg gaacggacgc ggaaagggcg ggtatcattg atggagggga
     2941 tatgacggcg gagtatgcgg tagctgtgat gatgaaaatg atttctgata tgcaaaagta
     3001 gcattctcgc aagaagcatt tcatatgctt gcatatgaaa gaaaatcagc ttgaaaaaaa
     3061 tgtataattc gactgttctg acggattttt ttgaaatcct gcattttttg cttgcaaaat
     3121 caaggagaat gtagtacaat taacatatat gaaacattgg caaaagccga aaggatataa
     3181 aaatgcagga agatctactt gttcagattg aaaagaggat gagcactttt tcaaaaagcc
     3241 agaagctcat agcaaacttc atcataaaca gctacgaaaa agcggcgtat atgacggcgt
     3301 taaagctcgg aaacgccgtg aatgtcagtg agtctacggt agtgcgtttt gctatagaaa
     3361 tggggtttga gggctatccc gaattgcaga agagccttca gtcgctgata aagaaccgtc
     3421 ttactgccgt acagcgtatc aatatcacaa atgatcgtat cggcgatgac ggtattttga
     3481 aaaatacgct gacgcaggat atggatcgta tccgcagaac gcttgaagag atcgacccca
     3541 aggtctttga cacggctatc gaaaagatat gttcggcaag gagaatatac atactcggtg
     3601 ctatgagcag cagtatcctg gcgagatttc tcgactacaa cttccagctg atatttgaca
     3661 acgtgcactt tgtacaggct atcaatacaa gcggtatcta tcagcagatc attcatatga
     3721 acagcgagga cgcatttatc gcgatatcat tcccacgcta ttcacagagt acggtaaagg
     3781 ctaccgctta tgcaaagagc tgtaatgcaa atgtcgttgc aataaccgac agtgtgactt
     3841 caccgctggc atcgtatgca aacgagctgc ttatagcaag gagcgatatg gcaagctttg
     3901 cggactctct tgtagccccg ctcagtctgc tcaatgcaat tatcgttgcg gtaagctcaa
     3961 ggaacaagga caatatcgaa cagaccttcg gcaagcttga aaagctgtgg gaggaaaatc
     4021 aggtatacaa aaaggacgta taatgaacta cgacttagca ataatcggcg ggggcgcggc
     4081 aggtatggct gccgccgttt ttgcctccga gagaggtgta cgcaccgttg taatagagca
     4141 caacagacag ctcggcaaaa agcttgccat aacaggcaag ggcaggtgca acgtcaccaa
     4201 caacagtgac aacgacaata taatgaagaa catacccaca ggaagccgct ttctatacag
     4261 cgctcttgcg ggtttttcgg catatgatac catgagcttc tttgaggggc tgggagtgcc
     4321 gctgaaaacc gagagaggaa accgtgtttt ccctgtttcc gacagtgcaa aggacattgt
     4381 tgaagcgctt aaaaagcgca tgggtacgct cggtgtcaaa ataatcaccg cgcacgcaaa
     4441 aggggtaata accgaggacg gcag
//
LOCUS       DAWAOG010000112         4447 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2523_length_4447_cov_2.189663, whole genome shotgun sequence.
ACCESSION   DAWAOG010000112 DAWAOG010000000
VERSION     DAWAOG010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2523_length_4447_cov_2.189663"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2331..2340
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1215 a         1034 c         1007 g         1181 t
ORIGIN      
        1 tttctgcggt tcttcatcac tctgagagtt gaaccgtcct cagcttttat atcaaggctg
       61 tacatacgtt caagggtaac gcccttatcg gtaatatcct tttcctgctc atctgtcaga
      121 ggtaagaatg tcgtgaactg tccgtcctca acgtgattta cagcgttatg ctcgtcggtt
      181 ctgataataa tcgtttccga gcttgccacc atcgcagtca ccagatacat acaaagcact
      241 atcagaagca gcagtgcgaa gtacttaggg aggtttgacc tgagctccct cggtattctt
      301 tttgaaagca ccttgttcat attacagatc ctccagctcg gcggcaggta tcagcgtttc
      361 attataataa tcctttttga taagtccgtc ctttaagatt atcaccttgt gcaccatatt
      421 ctttatagcg ttgttatgcg ttactatcag catcgttgtg ccgtatttcc ggtttacctg
      481 ttcaagaaga acaagtatat ccctcgaagt ctttgaatca agcgcacccg ttggctcgtc
      541 acacagcagg agctttgggt tctttatcag tgctcttgct atggcacatc tctgctgctg
      601 accgcccgaa agctgtgagg gaaacttttt tctgtgctcg gtaagcccaa gcgtatcgag
      661 aagctcgttc atatcaagcg gcttatcggt aaggtactcg catacctgaa tgttctcctc
      721 gacagtaaga ttaggcacaa ggttataaaa ctggaatata aagccgaggt ttgcctttct
      781 gtactttgcc agagcgtcag ccttaagccc gaatatttcc gtgccgtcaa cctttacaga
      841 gccgctgtcc ataacatcaa gaccgccgat acagttaagc agtgtcgatt tacccgatcc
      901 gctcgttccc tgtattacgc acatttctcc tttttcaacg cttgtggtca cacccttcaa
      961 tacctgcgtg tagcctgcgt ccgagccgta gctcttcttt acgtctttta cttcaagata
     1021 catttctttg tcctcccgta tcattattag ttttatctaa ctgtattagt tattgctaac
     1081 ataacagcgt tatcctattg tgcttttaat gaccgccgtg caagggcggt cagcattatt
     1141 catcttttac aaacaacaga tcaaaccaca tctttaccat tgcgccgatc attttctcgg
     1201 catacgcaag tcccttctcc ttttcgggaa cgtgggtaag cagatgtatg aacatctcta
     1261 tatccatatg ggcgagccaa tggagcatat actcgtcaac atgatagccg ggcatattct
     1321 taagcagcgc ctcggcactt gagcgaaagc ccacttccat aagtgcaaca aattcatcga
     1381 cgcagttttc atagaccgag ccttgcgact ttgtgagcag cagcaggaat gcgtcataat
     1441 tatcataaag acagcccacc atagtgacag cctctttatg agttgaaagc tgattttcta
     1501 cactcctcgt ctgctcgtcc tgcgagccta aatactcctc gtcctcacgg ttatgctcga
     1561 gcaacatctc tttcagcttt aaaagcggag gtgcaacgat cgcactgaac aaatcgtcct
     1621 tgtttttgaa aaagaagtac aaagcgcctg tcgtgacacc tgcgtttgtg cagatctttc
     1681 tgagcgacgc ttttgcgaag ccgttttcaa aaaattcctt ctttgcgctg tctatcagct
     1741 tctgtcttgt ttcgttatcg ctcataaagg cacttccttt ccgcagagat tttacataac
     1801 actgttatct taacctatat ccgtcataat gtcaataccc ctgacactat ttcatagcat
     1861 tgcaccaaaa cagcagcata acgctgttat gttattgtgc gtaattacaa caaatattct
     1921 ttcaggcggc atttcaagtg cgtttttaac tgtcttatca tatcagtcgg tcaattcgat
     1981 aaaattttaa cgatttttcc gaaatcggtt gacagcgctt acaattttta atataatatt
     2041 atatgtatag acagacggaa gaattgcagc cgatgtgctc aaataccttg aatgcagggc
     2101 gttttatctt ccgcacagaa aggcagaaca aatgaacggt acaatttcta agcaggcact
     2161 gaggagattg cccttctatc ttgactatct gaaaaaaaag cagacagacg gtgtgcgcaa
     2221 catctcggct acgactgttg caaacgacct taagctcaac gaagtgcagg tccgtaagga
     2281 tctggcggcg gtaagcctta cgggcggcaa gccaaaaacc ggctatgttg nnnnnnnnnn
     2341 ctatgttgcc gaagagctta tacgtgacct tgagagcttt ctcggatacg acaataccaa
     2401 agaagcggta atagcaggtg caggaaagct tgggcaggca cttctcgctt ataaggaatt
     2461 tgaacgctat ggacttaatg tggtagcggc gtttgatacc gacgagagta cggtcgacgg
     2521 caagcatatc cttccggcag aaaaaatgtc ggatatatgc tcacgtctta aaatacatat
     2581 cggcataatc tgcgtccctc cggaatccgc acagaaagtg tgcgatgaga tggtggaatg
     2641 cggaatactc gctatatgga attttgctcc cgtgcatttg tctgttccgt ccggcgtaat
     2701 agtccagtat gaaaatctgg cagcttctct tgcagtgctt tcacaaaatc tggagtttga
     2761 gatcaaggac agtttgattt cctgatctgc tctgcttatt tgaaaggaaa aagtattatg
     2821 aagaacatca gcatctgcgt aggaagctca tgccacttga aaggctcata taagataatt
     2881 gagctggcaa aagactacat cgcaaaccat aacgtcgggg ataaggtaaa cctcggtgcc
     2941 gctttctgtc ttggaaaatg cacagacggc gttacgatca aaatagatga tgagataatc
     3001 tgcggagtaa acgaaaacaa ctttcagcag atctttgacg aaaaggtact tgcctgtctt
     3061 gactgacagc ggtacgaaaa ttaaaaacag gaacggtgga tttaaatgct cacaacagga
     3121 ttgacgggaa aagcagaagt aaaggtcgat gatacaaata cggctgttac aatgggaagc
     3181 ggctcgctga aagtattcgc tactccggcg atgatcgcac ttattgaaaa ggctgcctgc
     3241 aaggcgctcg aaggacagct tgaggacgga cagaccacag tcggcacaaa gcttgacgtt
     3301 gcgcacattg ccgctactcc tgccggtatg aatgttacag cagaggctgc tcttaccgaa
     3361 gtcgacaacc gcagacttgt gtttgaggtc gttgcaaggg acgaaaagga catcatcggc
     3421 aagggtactc acgaacgctt tattgtgaac gcagaaaaat tcactgctaa aacttatggc
     3481 aaggtgtaat ctatgagtta tttacggctg aagaaatcca actgcaaaaa ctgctataaa
     3541 tgtataaggc actgcccggt caagtctatc cgcttttcgg acgggcaggc taatattgtc
     3601 gaggacgaat gtatactctg cggtatgtgc tttgtagcct gtccgcagaa cgcaaagcag
     3661 ataagaaacg atgtaaataa agcaaaagag cttatcaaca cgggtactcc cgtgtatatc
     3721 agcatcgctc cgtcatttgt cgcaaactat gacggcgttg gcataaactc aatcaacgat
     3781 gctcttaaaa agctgggctt tgccggggcg gaggaaaccg ccgagggtgc tcagcttgtt
     3841 accgcacagt atgaaaagct ggtagctgac ggcgagcaca agataataat cagctcctgc
     3901 tgtcacacgg taaacaccct tatacagaaa tactaccctc aggctctgcc gtatcttgca
     3961 caggtgaaat cgcccatgct tgcacacgga gagatactca agaagaaata tccgggctgt
     4021 cacaccgtat tcatcggtcc gtgcctttct aaaaaggcag aggctgacgc atatgcgggc
     4081 gcgatagact gtgtgctcac attcgaggag cttacgggct ggctcaagga agaaaatatt
     4141 gaagtctgtc ccatctccga caatgccgga agaagcgaca gggcaaagac aaggctgtat
     4201 ccgacaacat cgggcattct gcgctcgatg aacaaggaca accccgaata tctctatatg
     4261 gcgatagacg gcgtggagaa ctgccgtaac gctctgcgcg atattataga cggcaacgta
     4321 ggaaagtgct ttatagaaat gtccgcctgc gtcaacagct gtataggcgg tcctgctatg
     4381 gataaaaagc acgagggcgt tatccacgaa agaagagctg ttgatgagtt tgccggaagc
     4441 ggtaaat
//
LOCUS       DAWAOG010000113         4440 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2532_length_4440_cov_2.501710, whole genome shotgun sequence.
ACCESSION   DAWAOG010000113 DAWAOG010000000
VERSION     DAWAOG010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2532_length_4440_cov_2.501710"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    250..259
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1164..1173
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1057 a         1112 c          918 g         1333 t
ORIGIN      
        1 cgtatgcgcc gtgatatcct cgatccgcgc catatgccat atcgaatatg cgattgtctt
       61 gcagtgatat ccattggcgt tcgggcacgg cataaggctg tattgctcgt cacaaagcct
      121 tccgcacagc tcctttaccg catttgatag cgtgcttctg agcttcagac acgcctcaat
      181 tccgtccgca aatgtatccc ttttgcgcag aagcaatggc atttccttgt tgatctccga
      241 ccattctttn nnnnnnnnnt tcccctgcaa aattgccccc gaagcgcttc gggggcataa
      301 taattacttg tgcattattt caagattttt gctgataagc tcgcatctct gctttacgca
      361 gtcaaccgaa cggagcggat ctgtaggagt gttgaatgtg taatccatca gcttgtcgat
      421 cgtagtagcc gcaacgtgca gtcttgtatc ggaagatgca taatagatgt aaacatcgtc
      481 gttttcggtg acaacagcgc cgtttgtgaa gcatacgttc gatacgtcgc ccacacgctc
      541 gcccttatga ggagctatga acagacccta gggggaagct ataagctttg atggatcgtt
      601 aaggtcggta gcgaatacat agattacata tctcagtcct gccgcagtgt tacgaacacc
      661 gtgcgcaatg tgtatccaac ccttgggagt ctttatcgga acagcgcctg cgccgttctt
      721 gacctctgtg atcgtgtggt actgtctggg cgagatcaca acctcttcgg tgcagataac
      781 gggatcattg atatcctcgc acagaccgaa gcatacaccg ccacccgagc ctgtctgaat
      841 gaaatcgtcc tgaggacgtg tatagaaagc atatttgccg tcaacaaatt cagggtgaag
      901 aactacattt ctctgctgag gtgactttga cttcaggttg ggtaagcgct cccatgtctt
      961 caagtccttg gtacgaacaa tacccgcctg agctacagcg gcagaaaggt cattgctgtc
     1021 cttgtccttg ctctcggagc agaacacacc gtagatccag ccgtcctcgt gctgagtaag
     1081 gcgcatatcg tatacgtttg tttcttcctt ttccgtatcg ggaagctgaa cgggatagtc
     1141 gtggaaacgg aagccctctg tagnnnnnnn nnnaagaatg actttctgtc attgccctca
     1201 actctcgcta caagataata cttgccgtta agataaatcg cacccgagtt cagcacagcg
     1261 tttatgccaa gacgctccat aaaataggga tttgtcttgg ggttcatatc gtacttccag
     1321 aagtaaggag catgatcagc tgtcagaaca gggttcacat atctgtcata tatgccgtta
     1381 tagcaatctg caacggcgtt ctttctgttg ataagacgct cgtaatcgtc tatcactctc
     1441 ttcttgttat cttcaaataa gctcatttat taactatttt tcctttcggt ttatttgggt
     1501 tagctatcgg gaatagccac attttagatt atacaacagt taaacgcaaa atgcaatacc
     1561 ttttcttaaa tttacttaaa gaaatttcga ttttaagtaa attactcttt ttcggctttt
     1621 tcagacgctt cagacactgc cgcataggta taatctgaaa tattcgtact gaatatcgtg
     1681 acatagatcc ctgctccgca cacacagcag aacagaatgt ttacaaacat cgcgatttct
     1741 gccgctgtat cgctgtaggt cagcgccgag ctcaactgca tcaccgcaat caaaaggttg
     1801 ataacttcgg caagtataaa cgaaacagca gcagccggtc ttgcaccttt cttctttacg
     1861 gcacagacat tggtggctat cgccataccg atatttatac ataggataat aaatattatc
     1921 atcgttgtga taaacattat cgccgactcg ggcagagcaa aatcatcgac ctttttgggg
     1981 tcggttataa agaatataga gcgcacaaga tttctcagat acagcggata caaagccgca
     2041 gtgataaccg cgtttgcgta cagtgcaaca acgcttgcta tcataaacag cttgccttta
     2101 tgcagttctt tctgctgacg ctcaaaaaac tcctgctgca gcttttcctt ttcttcgttt
     2161 gtgagcgttc cgtccgaaat tatcgtatcc tgatttttca ctttaccctc cgatgttatt
     2221 cgttaattct atgtacttac tttgtgagtg cttcagtttc tttctcatct tcgttttcag
     2281 tctttacagc ggcttcgccc ttcagaaggt ctcgtatctc ggtaagcagt tcgatctgcg
     2341 gatcgggagc aggctcttct tccggctctt cctctttctt tcttttaaac gcgccgagca
     2401 cctttatcag aatgaatacc gacagtgcaa taagcaggaa actgataacg gagttgataa
     2461 acgcgccgta tcctatagcg acctcgggtt caactatctc cgtaccctct ttcactgccg
     2521 ccttaagcac tatcttaaga tccgagaaat caatctgacc tgtgatcata ccgagcgcag
     2581 gcatgagaat gttgtttaca agtgaggtaa cgatcgctgt gaaagccgca ccgactatca
     2641 tacctactgc catatccatt acgttgccct tggatatgaa gtccttgaac tcgcttacga
     2701 acttgatctt tccgaccttt tcaacgccat ggcctacagc ttttaacgcc tttacgtcac
     2761 gcagatcctt gtgttctttc ttctcgtttg ccattttaat tcttctcctc tcgattttta
     2821 cttattattc ggctttatcg ccgttcgagt tgtctgtgta gaccttgata agttcaaaat
     2881 caatctgtcc taagttgaca tttgcgttga tgcactttac acgaaccttg tcacctatcg
     2941 tgtacattgt gcctgtaacg ccgtcataaa gcgcaatacc gtttcttact tcaaaataat
     3001 tttccgacaa tgaacgtaca tcgatcttac cctcaacggt attcggaagc tcaacgaaaa
     3061 gtccgctgcc gataacgccc gaaatgatgc cgtcaaattc ctctcctatg tgctttttca
     3121 tatattcagc cgcatagaaa tcctcacagc ttctttctgc cgctactgcg gaaagctctg
     3181 tctttgttgc ctgagccgcc gccttgaccg agaactcacc gtatttcttc ttaagctttt
     3241 ccgcgccctt tcttgaaaca tagtcgctca ggatacggtg gatagaaagg tcggcgtaac
     3301 gtcttatcgg ggaagtaaag tgtgcgtatt ccttcattac aagtccgaag tgaccgatag
     3361 gctgttccga atacttagcc ttagccatag tacgcaatac aatgcggttg ataattacgt
     3421 ttctggggtc gtccgcagtt tcttcaagca gctttgcaaa gctttttgct tcagcctttt
     3481 cgtttattcc aagcggattt atgccgagcg cctcaagagt tgttttcagt gattctatct
     3541 tttcggcagg gggattttca tgcacacggt acacaaaggg gatctccttt ttcataccga
     3601 ccttagcggc ggaattgttc gccataagca taaattcctc aataataccc tcggacacac
     3661 cctgaaccct gggcttgata tcgatacaaa ttccgttctc gtcacatatc accttgctct
     3721 cactggactg tatctcgggt gcacctctgt ccttgcgttt ctttatcagt atctgcgcaa
     3781 gctcgttcat aagcggtatg cagtctataa cttccttata cttcttcttt gtcaaagctg
     3841 ttgccgtgcc gtcaaggata gaatttacct cgctgtacac accctgtacg cgcgaacgga
     3901 taaccgtctt tgcaaacttg tatttcttta cagtgccctt gtccgatatc tccataaggc
     3961 atgaaaaagc aagcctgtcc actccgggat tgagtgagca tatgccgttg ctgagctcct
     4021 tcgggagcat cggtatgact ttgtcggcaa tatatacgct tgtgcctctt tcaaatgcgt
     4081 cgttatcaag cggcgagccc tctgttacat agtggcttac atcggcgata tgaacgccga
     4141 gcttatatcc gccgtcaatg cgttcaacgc ttatcgcatc gtctatatcc ttggtatccg
     4201 cgccatctat agtgaatatt ggcaggtcac gcaggtcaag tcttttagcc gcctgcttct
     4261 cgtcaataac ggcattgaca tactgctttg cctgatctcg cgcctcttcc gaaaaatcaa
     4321 cgggtatatt gttctgaata agatatgctt ctgtagcgga tttagcatat tcgcttgagc
     4381 cgagcacctt tactatatca acaatatgct ccgagtggtg ttctccgcgc ttccttatag
//
LOCUS       DAWAOG010000114         4434 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2537_length_4434_cov_2.058918, whole genome shotgun sequence.
ACCESSION   DAWAOG010000114 DAWAOG010000000
VERSION     DAWAOG010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2537_length_4434_cov_2.058918"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1391 a          822 c         1045 g         1176 t
ORIGIN      
        1 ggatctcggt gatatttttg gcgtgagcgg ttttgtgttc aagacaagac gaggcgagat
       61 aagtgtacac gcaaagtcaa taaagctcct ttcaaagtcg cttctgcccc tgcctgaaaa
      121 gtggcacgga ctgaaggaca aggaagagcg ttacagaaag cgttatcttg accttatcgc
      181 aaaccccgag ataaaggata ccttcgtcaa gcgttcaatg atactgcgtg agatacgcag
      241 cttccttgac agcaagggtt atcttgaggt tgatacaccc gtactgcaca cacttgaaat
      301 aggtgcggct gcaagaccct tcgtaacaca ccacaacgct cttgacattg atatgtatct
      361 gcgtatcgaa acggagcttt acttaaagcg tcttatcgta ggcggatttg acagggtata
      421 tgaagtaggc cgtatcttca gaaacgaggg tatggacgca actcacaacc ccgaatttac
      481 atctatcgaa atgtatcagg catacacaga ctactacggt atgatggatc tgatagaaga
      541 aatgtaccgt acagtagcgc tcaaggtttg cggcacggat acagttcctt atcagggtaa
      601 tgaaatcgag ataggcaagc cctggaagcg tatgactatg aaggaagccg taaaggagta
      661 cacaggtctt gactatgaca gctggacaag tgatgccgac gcagtagctc aggctaagac
      721 aaagcacgca gagatcgccg acacggctac aaagggcgag gtgcttatcg agctgttcga
      781 ggaatttgta gaggacaagc ttattcagcc tactatcatc tatgattatc ctgtagaaaa
      841 ttcgcctctt gcaaagagaa agcccaccga ccccgcattc acggagcgtt ttgaatactt
      901 catcaatgcg tgcgaattcg gcaacgcatt ctcagagctt aacgacccca tcgaccagcg
      961 tgagcgtttc acaaagcagg ttatggagaa gagaaagcag ggcgcaaacg ctcagataga
     1021 cgaggacttc ttaacagcac tcgaatatgg cttaccccca acgggcggtc ttggtatggg
     1081 tcttgacaga tttgtaatgc ttcttacaga cagtgcaagc ataagagatg tactgctgtt
     1141 cccgacgatg aagcccctct cggactaaaa gcccataaaa atcaggtttt gacagcgttt
     1201 gcggcgtttt gttaatccaa aaatgaaaca cgtcagaggg tttacgacaa atttacgaca
     1261 aatgactttt tgagcatcac tcgtcttttg cgacaaatgc gtgtcgtaaa tcactggaat
     1321 cgtgaattgt taatccggtg gcttcattgt aacggctgca ttgtaaattg aacaaaaaag
     1381 ccttgtaaac tcggcatcta acgatgttta tgacagaacc tcggactgta tagttcgggg
     1441 ttcttttttg catatttgaa gccggcaacg attatggata tagtgtaatc gaatggaggg
     1501 actacaaatg aagaagtgtt atatcaagct gaccgagcag gaacgctctc aaatcataca
     1561 ttcgcttata gataagaaga acgcacttat ggctcaaggc agatacaccg acgctgttga
     1621 tgaagtcatt ttaaggctga tgaaagcaaa gaaaaaggct ttcatagtgc gtactgtttc
     1681 gtagtcattt ccttttcaaa aaataaaata tagatctata taagcaagcc gcttattcct
     1741 ttatctggat aggcggcgtt tttatttttc cgacacaaaa aaattttcaa acttttttca
     1801 aaaacacccg ataatttgac ctctcccagt ccgaacaagt gaaagggtta tttcttaact
     1861 ccgattagga gcaagtcttt tgctgttcct tgacaactga atataaagga aatgatgggg
     1921 ttgtaacgag aacttagggg aaaatgcgcc taagttttaa gtgtacctga ttaattttga
     1981 aaatcttcaa ttttgctgtt gtattttacg aagaattatg ctacaatata tacagcagaa
     2041 aggtaggtgc agacattatg ccgaatatta aacccgtatc cgacttgaga aactataccg
     2101 aggtactgcg tgacattgcg gtaggagagc ctgtgtttct tactaaaaac ggcagaggta
     2161 gatatgctat tgtcgatatt gaggaatacg aaaagacaaa agcgaccttg aagctgatgg
     2221 gcgaattatc caaaggcgag ctttcgggaa aggaaaaagg ctggactgag cttgcagagg
     2281 cagaaaaaat gctgggtata ataaatgact gagataaaat tttcgccgga agcaattgcc
     2341 gatttacaaa atacgaaatc gtatattgcc gatgagcttt gcaacgaaca ggctgctgtc
     2401 agtacgattg aaaagatttt aaagcgtatt cgtatgctaa aagattttcc cgaactcggc
     2461 gcaccgctgt catctatcgt aaatctcgat accgactacc gttttcttgt ctgcggaaac
     2521 tacacggcat tttaccgtgt ggaaaatgat acggtatata ttttgcgtgt gctttacgcc
     2581 agacgggatt ttatgcggat cctgttcggg acaccgcaag acaaaaacta aacatttcac
     2641 aaaaatcgtc atctgaaaag gatggcgatt ttttatatct gaaagaagga tttaatttgg
     2701 aagtaaaaat aaacagagaa atccgcaact acacagagtc tatgttcttt gggctgtcgc
     2761 ttcggcagtt cattttttct gcccttgctt gtgctgttgc ggtggtcata tattttctgc
     2821 tcagtccgta tgtcggcacc gagaccgttt cgtggatgtg cattttagcc gccgcccctt
     2881 tcgcggcgct cggctttgtt aagtacaacg gaataaccgc agaaaaattt ctgtggacgt
     2941 ggattaagag cgagtttctt ataccgaaaa agctcgtgtt ccgtccaaca aacacctatc
     3001 ttgagctgat gaagccgggt atcgaacaga aaaacaagga ggcgcttaaa acaaatgatt
     3061 aagacacttg caaaactatt taaacaggaa aaggagaagt tcgtagtgcc aaagggcgta
     3121 caggacgtca ttcctatcaa ggctatctat gacgacggta ttttcaaggt cgggaaagac
     3181 aaattctcca atgtcaatac agaccgtcat gcaggaaata gacacggagt atgaaaacag
     3241 gctgtccgag ataaaggaaa gcaatactta tgatattatt gaaatgagcg gaagcaaggc
     3301 agagtggaaa gaggtactca ccgtgtattc agttaaagtc aatactgata ctgacaaccc
     3361 gcaggaggtt gtcactatgg acgagggcaa gaggcagctt ttcaaagata ttttttggga
     3421 gatgaacagc atatcttcgc ggacggaaag caaagaggaa acggttgtga ctgaaagcga
     3481 tgacgggaat gggaatgcgg tgaggacaga aactactgtg gcgaaaacat atttgtatat
     3541 caccgtgaca cataaaacga ccgatgaaat ggcggaggag tatgggttca gtaaggaaca
     3601 gagggaaatg attgaggtgt ttttgaggga ggagaatgag tatttgtggg aggggtggga
     3661 ggaatagctg aaaggatttg attgtgagac ggcgtgaaat gagaaaaagg ttaataaagc
     3721 taacatagct taaaatcctt gacaataaaa tatataaatg ttacaattta atcatcggat
     3781 cattatgctt attgcgtgat acgaatttta aagggtgatg atatatatga aaatagcaat
     3841 atgcgatgat gagcggatat ttacagatat tttccgtgaa aagcttgaaa tgaccgccga
     3901 aaaatgcggt atagaatgta ctgtttcagc tttcttaaat ggtgctgaaa tgataagtat
     3961 gcttaatagg tttgatgcgg tatttctgga tgtagatatg ccggggctca gcggagagga
     4021 gacggcagaa tatattatcg gtttggaaaa aactctgccg attattttcg taactaatca
     4081 cgacgatttt gtgttttctt cattcagata cagacctttc ggatttttac ggaaaagata
     4141 tatcgacagt gagcttgaag atattatcaa acggcttgat aaatatctgg ctgagataga
     4201 aagatgttat acattcaatt cacacaataa gctgatttct gtacgattca atgatattaa
     4261 ttatattgag gtttacggtc atgaaacact tgtacatacc gcaaatgcgg tattaacgac
     4321 ttcaaaagct ctttccgcaa ttgaaaagga actgtccgaa tatggcttta tcagaactca
     4381 taaatcatac atcgtaaata cccgttatat tttttctgtt gaacgcaaca gcat
//
LOCUS       DAWAOG010000115         4420 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2547_length_4420_cov_2.236426, whole genome shotgun sequence.
ACCESSION   DAWAOG010000115 DAWAOG010000000
VERSION     DAWAOG010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2547_length_4420_cov_2.236426"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1428..1437
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1237 a          950 c         1003 g         1220 t
ORIGIN      
        1 tgaatttaag acagaggtaa acaagcagta ccccgattac agcggtgctg actatctcga
       61 tatgaccgca ggcttcttct tcgcataata gttcttatgc tgttcaatta caatttataa
      121 aatcacatag ggttgttgaa taacatttcg acaaccctat gacttatgag gtgagattat
      181 ggactatcag gaatatatcg taaacgacat tcacacggta atagccgcga caacggacaa
      241 aaaaggactg cctgttacct gcgccattga cataatggac tgtgacgatg acggattgta
      301 ttttctgacg gctaaaggct tatggcaatg tagcgaaccg tgttgcaaca aaacctttga
      361 caacgaaagc gccgtgcgga ctatggtaaa acagcagcag catatgaaga tccccgactg
      421 tcttatcccg agatgtcctc actgcggcaa gcctatggct atgaacctga gatgtgacga
      481 cagctttgta caggacgagg gctggtatgc cgccgcagag cgttatgagg aatttatacg
      541 caggcataag aatcttcaca ttctgttttt ggagctcggc gtaggattta acacccccgg
      601 cataataaaa tatccgttta tgcagatgac ggcgagaaat ccgaaaacgg tatacgcttg
      661 tgtcaatatg aatgaggcgt atgcaccgag ggggattggc gacagggcgg tttgtgttga
      721 cggggatatc ggtgatgtga tcgaaagctg cgttaagggg cagacttaaa tataagtaaa
      781 aataaaacga ctaagtgtgt ttcttagtcg tttttgtgtt tgcggagata agataactct
      841 ataaatcccg atttacctgt tcagtataat tatgagccgc agcaagaaag ccgcggctca
      901 acgttattat attatcatcg cgtttaaact caggcgatag ccaaagctgt tacactgaac
      961 atactaggac cgtctgtggt cttaggaacg atctttacaa tatgctcgcc tgccgtatca
     1021 aagctcttaa gctgtgcgca ttcaacatag ttgccccaac cgcctgtgaa gtcagcattc
     1081 agtgtcgtta caagctcatc atctatatag atatcagctg taccgcccgc ctttgtgtac
     1141 ttgccgtaaa ggatgcctat ctcctttgcg ttcaccttga aggtgagatt tgttgagctt
     1201 aaatccgtct tgtttgagcc cttgaagaac cagtagccgt caaagccgcc gaagtttgct
     1261 tcgttcactt tgaactcgcc gttcattgta actgcgtcac ctgcctgtgc aagtgtcagg
     1321 agagagccgt tttcaaacga tgctttcgta gcgggagttg agaagtcgct ctcgtcaccg
     1381 cttatgctgt caatatcctt gtcgacatct gaaatataag cctgaagnnn nnnnnnnctg
     1441 tgatactatc ttgtgaccgg gcacgttggg gtgaatatca tcgtcagaga tatctttcca
     1501 cataatatcg ccgtgcttga tcgtatcaag gatagtgttc ttgtacgata tcataggcag
     1561 atcgtagtgc tttacgatct ccgcgtgctg atcctggaag cttgtgccgt tttcctgtgt
     1621 tgtagctatt gtaatgatag cgggatttgt tgccgacttc cagatagttc tcagcaggct
     1681 gtcgtatgaa tccttgttgc gctctgtgtt ttctgttgta tcatttaccg agaactcaac
     1741 aaacacaaga tccggattct tcgacagaag atctgcggtt gctctgtgta caccgatgta
     1801 tgaaccggta gcgcctattc ccgcatttac atagttcact tttgcacttg aatatgtctg
     1861 ctgtaaccag tcagctgtaa gctttgcata gcaaagctca tttccggcgc ttgtgccctg
     1921 tgtgatagag ccgccgataa agcctaccgt tacctcctca ccgtttttga gcttctttat
     1981 tacatttgca agtcttacct tgttaccctc gttaagacgc gacatatcta tcatatgctg
     2041 tgtaagatcg ccgtccagag tgtactttgt ataatttgtc tccaagtcct ttgactcctc
     2101 ctgtgaactg tcgtctttgc tttcttctgc gctgttgtca gagctttctt ctgcggaaga
     2161 tacgtctgac tcttctgctt tgctgcttgt ttcagagctt tcggctgccg atgatgccga
     2221 accgcttccg ttgccgcttg ttgcattgcc tgcacaacct gttacagata ccgcaagagc
     2281 ggctgtaatg agtatcgcaa gtattctttt cttcatatta ctgcctttcc ttaaacaaat
     2341 ttaagttctg ttaagcgatt tttaggtaat cactcacact aatatatcac tatttttacc
     2401 gaatgtcaag ataaacaggt aaaattttct gagcaatttt tacataattc agttgaaaac
     2461 gatggcgtaa tatgttaaaa taaatacaag ccgattttac ttcggcagta tgataaaatt
     2521 aagcgaggaa taagataaat gactgataag aagataatca gcccggcaaa cgaggcgttt
     2581 tcgtacatgg gcaggatcga tttttcaaag ccggacagcc ccctgttcat ctatgcggga
     2641 agtatggtga gcacaaaatt cacaggaaca tcggtcggaa tgatgatttt tccacacctc
     2701 atatataaag taacgtggat cggcgtgata gtagacggta tccaatacaa gtttgacctg
     2761 aaccattccg atactcccgt atacattccg atagttgacg gacttgagga cggcgagcat
     2821 actattacca tattcaagag aacggccggt tcgcatcact acttcaattt ctgcggactc
     2881 atgatcgaca gcaacgcaga agtaaagccc gactgtcaca aatatgatat gaatatcgaa
     2941 gtatacggcg acagcgtatc tgcaggcgag gtagtagagg cagtaaacta ttgcgagcat
     3001 accgaccccg agcatatcaa ccagtttgat aacagctggt tctcatatgc gctccagctt
     3061 gcgagaaaac tcaatgcgcg cataaactgc aacgcacagg gcggtatttc gcttataaac
     3121 ggcgcaggat attttgataa ctatgtaggc atggaaacgg tttataagca gatgggctac
     3181 gagccgcact ttgagcttac cgactgggat ttctcgcgct tcacacccga ctatgtaata
     3241 atcgcgctcg gtcagaatga tgcaaatccc gacccaaagc gtatatatga aaaggaatac
     3301 cgcgaaaaat ggaagacggt gtataagaat atggtgcttg accttaacga aaagtactgc
     3361 ggcaaggcaa gatttatagt catcactacc gttcttatgc atgaacctac atgggacgat
     3421 gcacttgatg agatagtaaa cgagcttgct atggataatg tcaagcgctt taagttcaga
     3481 agaaacggtg cggcaacccc cggtcaccac cgtatccccg agcagaccga aatggcagat
     3541 gaactgtaca gctttatcac ttcttgggaa aagtaattgt aattttgcac aaaaagcaag
     3601 gttgaaacaa ggcacaattc ataaaatcat cacaaatcta ttgaaagctt ttgggaattg
     3661 tgctattatt aagttattaa aatataatgt taggagtatc tgttatggct attaacgtgc
     3721 ctaaggaata tgaagttccg ctttatcttt tccatcaggg aaaaaatgcc gaagcgtaca
     3781 aacttttcgg ttctcatttt accgaaaaga acggtgtaag cggtgtagtt ttcagagtct
     3841 gggctccgaa agccgctgat gtctgtgtag taggtgactt caaccactgg aaccgcgaag
     3901 aaaactatat gaacaagata agtgacggcg gtatctggga gcttttcatt ccgggactca
     3961 aaaagtttga taactacaag tacagcatca ggactgccag aggtcagata aagcttaaag
     4021 ccgacccata cggctatcat atggaaactc gcccgaatac cgctacaaag gtatatgaca
     4081 tttccggata caagtggaaa gacacaaagt ggatgaatga taaaaagaca aaagatgtct
     4141 accgttcacc gatgaatata tatgaggtcc atctcaattc atggtttaca aaaaccgacg
     4201 aggaagagct gttcagctat atgcagcttg cggaaaagct ggttccctat gtaaaggaaa
     4261 tgggttatac ccatattgag atgatgccga tagcggagtt cccgtttgac ggttcgtggg
     4321 gataccagca gataggctat tacgctccca catcacgttt cggaacaccg tcagacttta
     4381 tggaatttgt cgactactgc catactcacg gagtaggcgt
//
LOCUS       DAWAOG010000116         4408 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2557_length_4408_cov_2.463129, whole genome shotgun sequence.
ACCESSION   DAWAOG010000116 DAWAOG010000000
VERSION     DAWAOG010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2557_length_4408_cov_2.463129"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1363 a          944 c         1123 g          978 t
ORIGIN      
        1 aaacacttct gccgatggat atatcgttga gatgtatcag ggcgaaaatt gggtgagtgt
       61 tgccaagata acagataaca gcacgaccac attcagaaaa gcaggccttg ctccttcgac
      121 ggtttataag ttcagggtaa gagcgtataa gatagatggt aaggcggagc tttacggtaa
      181 ttacagctca atggttacgg caagaactaa tccatctgta attacaggtg ctgtactcgg
      241 cggaagagct gccgatgcac tcagagtgaa ctggagcaaa aacacttctg ccgatggata
      301 tatcgttgag atgtatcagg gcgaaaattg ggtgcgtgtt gccaagataa cagataacag
      361 cacgaccaca ttcagaaaag caggtcttga tgcttcgacg gtttataagt tcagggtaag
      421 agcgtataag atggatggta cgacagcgct ttatggtaat tacagttcaa cggttacggc
      481 aagaacaaat ccgtcggtaa tgacaggtgt taaaataggc ggcgttgcca atgacgcgct
      541 gagagtaaac tggaataaga atgcttcggc acaaggatac atcattgaaa tgtatcaggg
      601 tgaaaagtgg ctgcgtgttg caaagatcac tgacaataat acgaccactt tcagaaaggc
      661 aggacttgct aagaatacga cttacaagtt cagagtcaag gcttattata tgagcggtaa
      721 gacggcactt tacggtaatt acggaagtgt cagcggaaag actgcggcaa aataaaggca
      781 taaagctaat agcgacaggg catcggtgca aaccgatgcc ctttttatgc ataaagatga
      841 aatcgcggcg gcggacgaat acaagcggca aaaccgcaaa taataccggc aagcactatt
      901 agtcaatatg cacaaaaaac aggcaggttt ttgagtataa attctatctt gattttttta
      961 aaaaaggctt gtattccgca tcggaatgct gtataataaa tcttgcgcgt gaacgcgtac
     1021 tgcaatgata tgctgtgatg tctgaggttg cggctatacc ggtaattcag acggagtatg
     1081 tccagtgaat gggcgaaaac ggctttgcaa agcggttttc gtggctttat agaagataag
     1141 gatcgttctc ccgtcaagac gggaagggtt agcaataagg ttgatacccc cgagggaaaa
     1201 gatcctgttt tatatggaag cttacgaaac acccggcacg gtatgtttta taaataccgc
     1261 aagtcgtatc tttcccccgc aaatattacg aaagggagaa acaaaatggc agttaaggaa
     1321 aaagtgagaa tcaagcttaa aggctacgag cacgcaacag ttgatgcagc tgctgcaaag
     1381 atcgttgaaa cagctaagcg tacaggctca agagttgcag gtcctattcc gcttcccaca
     1441 aacaaggaag taataacgat cctgagagcc gtacacaagt acaaggacag ccgtgagcag
     1501 tttgagatga gaactcacaa gcgtcttatc gacgttatcc gccccaccaa caagacagtt
     1561 gaagctctgc agagcctcga acttcccgca ggtgtggaaa tgactatgga agcgtaagcc
     1621 atagcgagtt atgttggcgt aaagccaacc aataaccttc ggcaccggaa acccgaaaca
     1681 atatcggtgt cggtggtcaa gtcggaaaac cgaccaataa cctaaacagg aggatttaaa
     1741 ccatgactaa gggtttaatc ggaaagaaaa tcggaatgac tcagattttc gacgaagcag
     1801 gaaaagtagt tcctgttacc gttatcgaag caggtccttg tgttgtaaca cagctcaaga
     1861 cagctgagaa tgacggctat gaagcagttc agcttggttt cggcgattta tcaccgaagc
     1921 acgtaaacaa gcccatgaca ggtcacttca agaagaatga cctccccttc aagagaactc
     1981 tcaaggagtt cagattagac gacatcagca acgtaaacgt tggcgacgtt ctcaaggctg
     2041 acgtattcgc agcaggcgac gttatcgacg taagcggcgt tagcaagggt aagggcttcc
     2101 agggcgctat caagcgtcac aaccagcaca gacttaagga aactcacggt acaggtcctg
     2161 tagttagaca ggcaggttct atgggcgctt gctcatcacc ctcgagaata ttcaagggca
     2221 agggcatggc aggtcacatg ggtgctgaga acgtaacagt tcagaatctc gttatcgtta
     2281 agatcgatgc agaaaacaat cttatcgcaa tcaagggtgc tattcccggt cctaagggcg
     2341 gagttgtgtg cattaccgat gcggttaaga aagcgtaagg aggaggatca tcatggcaaa
     2401 agtatcagta tatgatatga caggagctgt tgtgtccgac cttgagctta atgacaacat
     2461 tttcggtatt gagccgaaca cagttgttat gcacgcagca gtggtaaatt acctggcaaa
     2521 tcagcgtcag ggtacacagt caacgcttac aagaacagaa gtaagcggcg gcggtaaaaa
     2581 gccttggcgt cagaagggca caggtcacgc aagacagggt tctaccagag ctccccagtg
     2641 gacacacggc ggtgttgctt taggccccaa gcccagaagc tacacatact ctttaaataa
     2701 aaaggtaaag agacttgcat taaagtctgc actttcggct aaggtgcagg acggcagcat
     2761 gatagttgtt gatgctatca cagctgacga attcaagaca aagacaatcg ttaatatgtt
     2821 aaaggctctc agcgctgaca aggcactcat cgttctcaac agcgttgacg aaaaggttat
     2881 caagagcgcc gctaatattc ccggcgtaaa gacaactcag gccaacactc ttaacgtata
     2941 cgacatctta aagtacaaca agttcatcgt tgttaaggat gccgttgcta ctatcgagga
     3001 ggtgtacgca taatggcaaa gcttgcttat gacatcattt taaagcccat catcacagag
     3061 aagagcatgg aaatgcttac tcagggcaag tacactttca aggttgccaa ggactccaac
     3121 aagatcgaga tagctaaggc tgtcgaggct ctcttccccg gtgttaaggt tgctaaggta
     3181 aacacagtaa actgccgcgg cgtaatgaag agaatgggca gaaacgaagg ttacacacct
     3241 gcttggaaaa aggcagtcgt aacactcgct gaaggctcaa agacgatcga gttctttgac
     3301 ggaatgatat aaaggagtga tataaatggc tattaagact tataaacctg tcacccccgg
     3361 tcgcagaggt atgactgtta ccgactacag cgttctgtca aaggttgagc ccgagagaag
     3421 cctgcttgag tcactcaaga agaactccgg ccgtaacagc tacggtagaa tcactgtaag
     3481 acacaagggc ggcgcacaga gaagaaaata tcgtgtaatc gactttaaga gaaacaagct
     3541 cggtatggat gctgaggtta tgactcttga gtacgatccc aaccgttcag cattcatcgc
     3601 actggttcag tacgaggacg gcgagaagag atacatcatc gctcccgacg gtctgaacgt
     3661 aggcgacaag gtaagaagcg gttccgacgc tgatatcaag cccggtaacg cacttgcact
     3721 tgctgatatc cccgtaggta cggttattca caatatagag ctttatcccg gtaagggcgg
     3781 tcagcttgta cgttccgcag gtaacatggc acagctcatg gctaaggaaa acggctacgc
     3841 tctgttaaga cttcccagcg gtgagttaag aaacgtatcc gacaagtgca tggctactat
     3901 cggtgttgta tctaacctcg accacgagaa cgttaactac ggtaaggcag gacgtgttcg
     3961 tcacatgggc gtcagaccta cagtaagagg ttctgtaatg aaccctaacg accaccccca
     4021 cggcggtggt gaaggtaagt cacccgttgg tcgtcccggc cctgtaactc cctggggcaa
     4081 gcccgctctc ggttataaga ccagaaagat cggcaagagc acagataagt tcatcgttaa
     4141 gagaagaaac agcaagtaat aactactaca agtcgttgtt aataagtgcg gcgtttgcgg
     4201 catagcagta aaaatccgcc gcatattaac ttgcaatcca cttgaaagga aattatacaa
     4261 tgagcagaag tatcaagaag ggaccttacg tgcaggaagc gctcatgaag agagtgctcg
     4321 ctatgaacga agcaggcgag aagaaggttc tcaagacctg gagccgttca agcacaatat
     4381 tccctgattt cgtcggacat acatttgc
//
LOCUS       DAWAOG010000117         4406 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2558_length_4406_cov_2.564238, whole genome shotgun sequence.
ACCESSION   DAWAOG010000117 DAWAOG010000000
VERSION     DAWAOG010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2558_length_4406_cov_2.564238"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1780..1789
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2329..2338
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1195 a         1063 c          952 g         1176 t
ORIGIN      
        1 ccatatcata gtcaacgaat atcgcgttga gatagctgtc gagcgtttct gcccagtagc
       61 tgtcgttgtc ggttttctgc tcatatacgc cggttatctt gacataataa ggctcaacac
      121 ctgccgcaag cgaaactatc ttatatgtat tgcccatatt tatgccaagc gttttgagcg
      181 catcttctga agcaagtccc tcgtatacgc cgtcaacaga ggtctttgag ggcagctcgc
      241 ctctgacgag cttcatatgg tcaagactgt cggtcattcc tataagcttc agccttgtag
      301 acagctccga accgttacgc tcgccgttta cgatataaag gcaatcgtca ataagcgttg
      361 tcttgctgtt ctccgaggcg attcctatag catttatcct tgaatccgac tttgatacaa
      421 catcgtctat cgttgcctgc tgagtattcg tatctgtcgt tatctgaagc gtctttgtaa
      481 cggaatacag accgggatat ttgttgttgt caatctggta ctgttccata tcctttatca
      541 gcatacgctg caaggacgcg tccatatata tcgggatagt gctcatcatg cccgccgcaa
      601 gcaaaaatcc tataaacagg catagtacca tccattttgt attgcgcatc ttgcgccaca
      661 gtaaagtaaa catctgtttt attccttcct taaggtaagt gtcggtgaat acaccgtatt
      721 actttacaac gacctcgtca ccctcggaaa gtcccgatgt tatctggatc tccgtagcgt
      781 tgcttattcc ggtctcaacg tcgacctctt tcttgacacc gtccttgtac acctgcacat
      841 agttcttgcc cgaaagggtc tttacaagat atttcggtat aactatcgca ttctccgcct
      901 gctccttaat gtagcttata tcagccaccg tgcctacctt tgtaaagctc ggaacagtgc
      961 ccttgaactc agcgtatata gacgatgtat cctcatcgcc ctgctccttt gcttctgtcg
     1021 gtgtctttac tatagtgccc ttgtagtctt cgttctcgat cgttatcgta acttcctgac
     1081 cctttgtgaa ttttttatcg tcgctgacag ctgcctttat atatatcttg tccggatcga
     1141 ttatagtagc tatcaccttg tacgactgta ccttcgtacc tgcattcaaa cgctccgcaa
     1201 agctcacaag tccgtcacac ggtgcataaa gcttagaacc gtcatattcg ttctgatact
     1261 gtgcaagcgt attcttctcg atctcaagct gtaatctgtc gttttccgca ccgctggaat
     1321 tatatgtatt ctgcgcctgc tggaccttta gctcctgtgt gcgtatctca tattcaaggt
     1381 cgcctgtgtt atactcggcg ataagatcgc ctttctttac ggtgtcaccg gctctgacat
     1441 atattgtctt taaagtgccg tcacgctccg taaaagagca gtcctgctgc acctccgaca
     1501 cacaatagcc ggtggcgctt gccttattga caattgtttt cttcactgcc ttgtaggtag
     1561 aatatgtaac atcttctact ttgagcacag gaggatcaag cagcttttcc tcctcaggaa
     1621 aaaagtagca gcctgaagcg gatgctgcca tggcacatat cacggcaacc gccgtaacac
     1681 gtataagagt ctttttcatt tttactttcc tttccttaag atcaagggat ttgcatcttt
     1741 taacttatat agaaaatcaa ggatcttgct gtccttatan nnnnnnnnng tccttatatg
     1801 gtcaaatctt aaaaaagact atagcgatgc ttattttaat tttagcattt ttcacataaa
     1861 ttttctatac tttttcaaaa ttaaacgata tgcacaaaac cgaaaaaggt tatttgttta
     1921 ttttgtacac cgcaaagccc gctcaaaaaa acgaaaaaca cttgaaaaag caaatcggat
     1981 ttagtataat agtgagtagt gtttattttt caaattgaca tataaaatca atacggcggg
     2041 cttttaaagc tcagggaagg gacaaaataa tggctacaaa gtacgtattc gtcaccggcg
     2101 gtgttgtgtc gggactcgga aaaggaataa cagcggcttc tctcggaaga ctgctcaaga
     2161 tgaggggcta caaggtaact atccagaaat tcgaccccta tataaacgtt gacccgggca
     2221 cgatgagccc ctatcagcac ggcgaagttt tcgtgacaga cgacggcgca gaaacagacc
     2281 ttgacctcgg tcactatgag cgtttcattg atgagaacct ctcaaagtnn nnnnnnnntg
     2341 gacagttctt aataaagagc gccgcggaga ttacctcggc ggtacggtac aggttatccc
     2401 ccacataaca aacgaaataa agtcaaggat ttacagagtt gcaaactcag ctgaaacgac
     2461 cgctgacgtt gttatcaccg agatcggcgg tacagtcggc gatatcgaga gcactccctt
     2521 ccttgaggct atccgtcagg tagtttccga tgtaggaaga gaaaacgttg tttatataca
     2581 cgttacactg ctccctttca tcacaggaag caacgagatg aaaacaaagc ccacacagca
     2641 cagcgtaaaa gagctccttt ccctcggtat ccagcccgat gtactcgttt gcagaagcga
     2701 gatggatatt cctcaggata tgacagagaa gatcgcaagc ttctgcaatg tccgcaagca
     2761 ggacgttatc cagaacctca ccgcgccctc tctttatgaa gtacctctta tgctcgaaaa
     2821 ggagggtctt gcaaaggtag tctgtgaaca cctcggactt gacccccgcg ttcccgacct
     2881 caccgaatgg gaagagatgg taaagcgcca gtacaatgct acaaaaaata ccgttatcgg
     2941 tcttgtcggc aagtatgttg ctctgcccga tgcctatctg tcggttatgg aatctctgcg
     3001 ccacgcaggt tttgaaaacg aatcacacgt tgagataaag cttataaatt cagaagaagt
     3061 aacccccgaa acagcagacg agcttctgca cgactgcgac ggcattctcg ttcccggcgg
     3121 cttcggcgac agaggaattg agggtaagat cgaagcagta cgttacgcaa gggaacacaa
     3181 aaagccctat ttcggacttt gcctcggtat gcatatggct accatcgaat ttgcacgtca
     3241 cgttgcagga ctgacagaag caaattcaag cgaatttaaa gagggcgacc agaacgttat
     3301 cgaccttatg cctgaccaga aggacgttga tatgggcggc acaatgcgtc ttggcttata
     3361 cccctgtaag cttgaaccag gcactatctc gcgcgaggtt tacggcgatg agctcatcta
     3421 cgaaagacac cgccaccgct acgagttcaa caacgtattc cgcaagcagc tgacagagag
     3481 cggtcttgtt atcgcaggtc agtcacccga cggaaaactt gttgagatag ttgagctttc
     3541 aagagatgtt cacccctggt tcgtggcggt tcagttccac cccgagttca agtcacgtcc
     3601 caacagagca caccccttat tcaaggactt tataagagcg tcgcttgaac aggcaaacaa
     3661 gtaaagagat ccgaaataac tgcccgtgaa agcgggcggt tatattcata ttacggggag
     3721 ttttacattc cctttacatt taaaaatata atgccaaaaa gcaaacgagg taattatgac
     3781 tgacagcaat gtatatgaca accgagaatt atcctggctg aaattcaacc ggcgcgtact
     3841 tgaagaagcg caggacgtaa gcgtccccct gtttgagcgt atgagattta tctcaatatt
     3901 cacaagcaac cttgacgaat ttttcatggt gcgtgtcggc tcactctacg accaggatct
     3961 ggtcgagcct aaaaaatgcg aaaacaagac aggtatgacc gccgcaagac agctcaacga
     4021 aatagcgcgc agagtaaagg atctgctcta tatcaaggac tgcgcgttca acgaggtcat
     4081 gtcaaagatg agcgaatatg cagaactgaa aaagcctgcc gatctgaaag gcgatgacaa
     4141 gctgttttta aggctgtact ttgaaagaga gatcttcccc ctgctttcgc cgagcattat
     4201 agacaagaac cacccgtttc cgttcttaaa aaacagagca atatatatcg gtactctgct
     4261 aaggagcaag aacgacgaga aaaagaaaca gcttgtcggc ataatgagtg ccgaatgcga
     4321 taaagatttc ccccgtgtta tctttctgcc gggcgaaaag ctccgctatg ttctcgcaga
     4381 ggacgtgatc ctgcactata tagata
//
LOCUS       DAWAOG010000118         4365 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2588_length_4365_cov_2.294200, whole genome shotgun sequence.
ACCESSION   DAWAOG010000118 DAWAOG010000000
VERSION     DAWAOG010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2588_length_4365_cov_2.294200"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1345 a          930 c         1112 g          978 t
ORIGIN      
        1 gttgcggata acacgggcgc taaagagctt atgtgcatca gagtactcgg cggtacacgc
       61 agaaagtacg ctaacatcgg tgacgtagta gttgcttccg taaagaaagc aagccccggc
      121 ggaactgtta agaagggcga tgttgtcaag gcagtagtcg ttcgttcagc aacaggcatc
      181 agacgtgatg acggaacata catccgtttt gatgaaaacg cagccgttat aataaaggaa
      241 gataagaacc ccagaggtac acgtattttt gggcccgtag ccagagagct gcgtgacaag
      301 gactatatga agatcctttc acttgctccc gaagtattat aatccggagg tcgtaaatga
      361 acacattaca catcaagaca ggcgataacg ttcagatcat gtccggcaaa gacaagggca
      421 agcagggcaa ggtacttgaa gtatcgccca aggaaaagaa ggttatcgtt gaaggcagaa
      481 acctcgtaac aaagcacgtt aagcccagaa gacagggcga acagggcggt cttgtaaagg
      541 cagaagctcc tatctatgcg tgcaaggtaa tgcctgtatg ccccaagtgc aacaagccta
      601 caagagtagg tcacaaggtt gagggcgaca agaaggtaag agtttgcaag cactgcggcg
      661 ctgagcttta atcagacgcc ccgaattacg gcagagaggt cggataaagt aacttccggc
      721 tgctgtcgct aaaggagaaa taaacgatat gacaagaatg aaagcatatt acagagaaac
      781 agttgctcct gctcttttca agaagttcgg ttacaaaagc acaatgcagg ttcctaagct
      841 cgacaagata ataatcaatg tcggctgcgg cgaagcaaag gaaaacgcca aggtagtaga
      901 cgctatcatg agcgacctta tgcagatcac aggtcagaag cccattgttt gccgtgctaa
      961 gaagtcagta gctaacttca agctcagaga aggaatggtt atcggtgtca aggtaacact
     1021 gagagatgaa agaatgtacg agttcctcga cagattcttc aatgtagctc tcccccgtgt
     1081 acgtgacttc agaggtatca accctaactc attcgacggc agaggcaact actctacagg
     1141 tatcaaggaa cagctgatat tccccgagat cgaatacgac aagatcgaca aggttcgcgg
     1201 tatggatatc aactttgtaa caacagctaa gactgacgaa gaagcaagag agctgctctc
     1261 acttatgggc gctccgttcg ctaagtaaga caaagaaagg aaactatcaa ctatggcaaa
     1321 gaagtcaatg attgctaaac agcagcgtcc tgcaaagttt tctacacgtt cttacaacag
     1381 atgcaagatc tgcggcagac ctcacgctta tatgcgtaag ttcggcatct gcagaatctg
     1441 cttcagagag cttgcttata aaggacagat ccccggcgta aagaaatcca gctggtaatt
     1501 taagaacaaa ttttcaagga ggaagtaagt aatgcagatc accgatacta tcgcagatat
     1561 gcttacaaga attcgtaatg ctaactctgc aaagcatcct acagttgatg tgcccgcttc
     1621 taacatgaag aagcagatcg cgcagatact cgttgatgag ggttacatta agagtttcag
     1681 agtgatagat gacgacaagc agggcgttat cagaatcacc ttaaagtata ctgagaacaa
     1741 gtcgcaggtc attacaggtt tacgccgtgt aagcaagccc ggacttagaa tttactcaaa
     1801 cagcaaggat atgcccaagg ttatgaaggg cttaggaatt gcaatcgtgt ctacctcaaa
     1861 gggtatcatg acagacagag aagcacgcaa gaacaacgtt ggcggcgaag tgctcgcttt
     1921 cgtttggtaa ttaaggagga acagatatgt caagaatagg taaacatcct atagctgttc
     1981 ctgccggcgt cgaagttaaa gtagacggtt ctacagttac agtaaaggga cctaagggta
     2041 cgctcacaaa ggagttcaag ccctctatgt cgatcgcagt tgacggcggt tttgttaccg
     2101 ttacacgtcc tgacgacgaa aaggaaaacc gtgcacttca cggtcttaca agaacactta
     2161 tccacaacat gatcgaaggc gttacaaacg gcttcaagaa ggagcttgac gtaaacggtg
     2221 tcggttaccg tgtacagaag cagggtaagg atcttataat gaacctcggt tactcacatc
     2281 aggtaatcgt atccgataca gaagacatca agatagagtc acccgcacct aacaagatca
     2341 ttatcacagg tattgataag cagaaggttg gtcagtttgc agctgaagta agaggcaagc
     2401 gtcctcccga gccttataag ggcaagggta tcaagtatac cgacgaaacg atccgccgca
     2461 aggaaggtaa aggaggtaag ggtaagaaat aaaccgcaag gggtgaaaaa taatggttaa
     2521 agtaatagac agcaagaaga gcagaatcaa gcgtcgcaag cgtatccgtg ctaagatcag
     2581 cggtactgca gcttgccccc gtctcagcgt tttccgttct gctaagcaca tttacgctca
     2641 gatcattgat gatgaagcag gcaagaccct ttgcagtgca tctacaatgg agaagagctt
     2701 tgaaggcttc ggcggcaacg cagaggcagc taaaaaggtt ggtcttgctc ttgcagaaaa
     2761 ggcaaaggca gcaggcatca gcgatgtagt attcgaccgt tcaggttatg tataccacgg
     2821 cagagttgcc gcgctcgcag atggcgctcg tgaaggcgga ctcaacttct aatcagtaag
     2881 gcaagggttt actgaaaccc gcaataaaaa caggaggaca agaatttggc acttttagat
     2941 gcaagcaatt atgagctttc cgaaaaggtt gttgctctga accgtgtatc caagactgtt
     3001 aagggcggac gtatcatgaa gttttccgca ctggttgttg ttggtgacgg caacggtgtc
     3061 gtaggcttcg gtatgggtaa gtcgaacgaa gttcccgacg ctatccgtaa gggccttgag
     3121 gacgctaaga agaacctcgt taagatctca ttaaagggta caacgatccc tcatgaaata
     3181 gtaggaaaat tcggcgcagg cgctgttctt atgaagcccg ctcccgaagg taccggcgtt
     3241 atcgcaggcg gtcccgttcg tgctgtcgta gaaatggcag gtatcaagaa cattcgtact
     3301 aagtcacttc gttctaacaa cccctgcaac gtagtaagag ctacaatggc aggtcttaca
     3361 gctctcagag atgcacagca ggtagctgag ctcagaggca agtctgtaaa agagatctta
     3421 ggttaagctt ttactttgca gaaaggacgg aaaccgaaat ggctttaaaa atcaagctgg
     3481 ttaaatcact ggttggacgt aaggacagcc acatcgcaac agcagcctct ctcggacttc
     3541 gcaagatcgg cgccgaaaca gttcagcccg acaatgcagc aacaagaggc aagataaaag
     3601 aaatttctta tttagttgag gttactgagg aataattcct tacaccttat agaggttaaa
     3661 taagcacatt aacaaatagg aggtgtaaca attgaagcta cacgaattat accctgcagc
     3721 aggtgcaaca aaggaagtta agcgtatcgg acgcggacac ggttccggtc acggtaagac
     3781 agccggtaaa ggtcacaagg gtcagtgggc acgttcaggc ggcggtgtaa gacccggatt
     3841 tgaaggtggt cagacatcac ttgcaagacg tatgcctaag agaggcttta acaatatctt
     3901 tgctacagaa tacactgttg taaacgtagc cgacctcgaa gctcgctttg agagcggtgc
     3961 tgttatcgat acagttgctc ttatcgaagc aggtcttgta actaaggttc ttgacggcgt
     4021 taaggtactt ggcaacggtg aaatcactaa gagctttaca gttaaggctg caaagttctc
     4081 tgaatcagct aaggctaaga tcgaaagtgc cggcggaaag gctgaggtag agtaatgttt
     4141 caagtcttta gaaacgcgtg ggcagacccc gcacttcgca aaaagatttt atttacattg
     4201 ctcataatag ttatattccg cttcggcgcg gcaatcttcg ttccattcct tgagccctca
     4261 actatcaaac agatgatggg tgaaggaacg atacttaatt atctcgacgt tatgacgggt
     4321 ggatcgctca gcaacggtac gctgttcgct atgtcgatca caccg
//
LOCUS       DAWAOG010000119         4358 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2592_length_4358_cov_2.385545, whole genome shotgun sequence.
ACCESSION   DAWAOG010000119 DAWAOG010000000
VERSION     DAWAOG010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2592_length_4358_cov_2.385545"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1270 a          943 c          827 g         1318 t
ORIGIN      
        1 ccctcttcca tagcccttgt tacagcatga tcaaacaacg agtcgctgtc gctcatctcc
       61 tcggacttta tcgggataac gccccacgaa agattaagct gacgctgtgc tctctcgctt
      121 gttgtagccg cgattatagg acaggtagga cggaactttg ataaagttct tgctgtacgt
      181 cctccctttg ttacagtaag tatcgctgtt gcaccgaggt ctatagaggt cgtgaccgtt
      241 gcgtgtgcta tggcgtttgt tacattcgga acaccctcgt tttcccttgt gaaaaaacgc
      301 ttgcggtaat ctatatcctg ctcggtcttc actgctatag ccttcattct ctttaccgca
      361 tcgataggat atgctcctgc ggcggtttcg cccgagagca ttatcgcact cgttccgtca
      421 tatatggcat ttgctacatc tgttgtttcc gctcttgtcg ggcggggatt ttttatcatc
      481 gagtcaagca tctgcgttgc ggttatgacc tgcttgcccg cattgtagcc cttatggata
      541 agctccttct gtattctcgg tatctcaaca aacggaattt caacacccat atctcctctt
      601 gcaaccatta taccgtctgc tacacgaaga atatcatcta tattgtcaac gccgtcctgg
      661 ttttcgatct ttgcaattat gcttatatcc ttgccgccgt tctcgtcaag cagctttctt
      721 atctgtataa tatcctcatc gcgcgttaca aagcttgcgg ctactatatc aaagcccgtc
      781 tttataccaa aaagcagatc gctcttgtca cgctcgctga gataaggcat tgaaagatgt
      841 atgccgggaa agttacagct cttgttgggc ttcaatgttc cgccgtggat aaccttacag
      901 cggatatcat ctcctgtttc cttgcggtcg atctctgtga ccttaagctc aagaagtccg
      961 tcatctataa gtatagtcac acctgtttca atatcgctag caagattggg atagtttata
     1021 gcggctctct ttgcatcacc gacaacatct tccgttgtga gcgtgtactc actgcccgtc
     1081 ttaagctcaa caggcttatc gtccttgaag ctctttatcc tgacctcagg accttttgta
     1141 tcaagaatag ccggaatcgg aagtcccagc tccttcctta ttctttcgac ctgatcataa
     1201 cgcttcttat gatcctcgtg cgtaccgtgc gaaaaattga aacgggcaca atccataccg
     1261 ctttttataa gctcacgcag tacattgtcg tcatctgttg caggacctaa ggtgcatact
     1321 atctttgtct ttttcataaa aatcctttct ttacagtaga tccgtctttg ccatttcagt
     1381 ctgtcaaaaa tatatataat ttttttaaat ggggtttggg gcgtagcccc aagcggggtg
     1441 agggcggcag ccccacactc agccccttcc cacggggaag gggtttgggg ttagggatag
     1501 agaatttgct ctatttttat aaaaacagac tttttctaca aactgcatta taagtataac
     1561 attttttgcc tttgatagca acagaaaaag cgcacacagt gacaatttta catgaacttt
     1621 acaaaagccg ctagatcata tcaccccggt caaaaaaagc acagccgaca ctatccccgc
     1681 gccggctgtt cctataagcg cacctattat aaaaagaaat ttactttttt gcccccgttt
     1741 tacttgcctt tcaaggctga taccgttaag atacagctgc gcctgatttc ccagcttgct
     1801 gttacacgac atttcggggc ggacatagaa gatcgccttt tcaaaatatt cgttctcggt
     1861 attgtttatc tcgagtacca gtttattcac cgcttttacc aatttgcaca ctccttgttt
     1921 tcaactttcg cagattacaa attgtattat aggaaatata tgttaaatta tacgcgttgg
     1981 atattagttt tcaacagaaa agtgtggaaa actgcggtgt tttcaactta tgcaggctaa
     2041 aatcagtcag aaacagttga aaactcggtt gaaattgtta aaacttccga taaaaacgga
     2101 tttaaaactt tttatcaaat gcagttgaaa ctaaaaaaat aattttacaa ataatgctat
     2161 ttagtgacat tttaaggtaa ataaataaga atggttttta agttgaaaat ttcgtattgt
     2221 cataatgtat attgtaacgg ttacacgaag ttcagctgtt gataacatag cgtataatgc
     2281 accgtttttc ccgcttttca acaggtaaaa cggttgaaac tgttgaaaac tacctgtttt
     2341 caacctctta acggtgcaaa tgtagctttt tttgttgaaa actccgttga atatgtgaat
     2401 aacttcgctt aaaatgggct tgtacgggtg ttaataagaa gaaaaactat tttaaacagc
     2461 aggttaaaaa taaaatttac atccgcctta atttccattt acatcagaat atctgcttgc
     2521 tgacctgata tttttgtgtt gtcaggctcc cgaaagttct ctttgcaaaa cagagcttct
     2581 gtttttccat tgttaataaa caaaatttaa attctgcacg ctgaattttt cattcctgat
     2641 ttttcatttt tcattcttca ttacatatca tctcccactt gacaccccgc caataatatg
     2701 gtattataat cttaattatt cttttaataa aaatcgcagt ttttcgccgt ttttttacac
     2761 atatttttag gaatttactt acattttata gatattgttg tggttttttt aaaaacagag
     2821 gtgtgatgat gagcttttat aattcctttg aggaaatttt tcaggagatg aaaaagagac
     2881 ttactcttac tcctaccgcc atgaaattat ggatcgagcc gttaaagccg ctcaaactca
     2941 ataataatca tgtcctgcta tatgtagaga gccgtttttc aaaagatatg acaatggcaa
     3001 atttcaagac cgttatggaa gaagcattca gcgatcttct gggttttgac gttgaggtcg
     3061 aaatactctg ttccgaagac cttaatgtag aacaaaagct caagtgcggt atcgagtcat
     3121 atgcagattc ttccccgaaa gaactgcaaa aagaacttga agatgacgat attgtaaaga
     3181 cccgccttaa gaacagcgag cttgcaagca attatcagca tacctttgat acatttatag
     3241 taggtgacaa caataagctt gcatatgccg cctgcaaggc agtagtacaa gaaccgagca
     3301 caagatataa cccgctttat atctacagcg aacccggact cggaaaaacg caccttcttt
     3361 cagcggtaaa aagcgagatg gaaaagctgc atcccgagat gaatatcatt tacaccacag
     3421 ccgatacctt tacagacgac tatgtaagca acctgcgtac aaaaagtaat cttgaagcgt
     3481 tcaagcagaa gtaccgcagc tgtgacctgc tccttataga tgatattcag ttcatggcta
     3541 ataagaacga aacacagcag gagctgttcc atacgttcaa cagtctttat aatcagaata
     3601 aacagatcat atttacctca gatagaccac cgaaggagct caacggcata gaacaacgtc
     3661 ttataagccg ttttgaacag ggcttgcttg cggacattgc accgcctgag tacgaaacaa
     3721 gaattgctat aatcaagcgt aaggctgagc tttacggtat gaatcttccc gattctctag
     3781 tagactttct tgcggacaag cttaaaacaa atataagaca gcttgaagga gctattacaa
     3841 aaataaacgc actgacgctt gttacaggct caactcccac tcttaatatg gcacagcagg
     3901 tagtccgtga catacagtca aatcatgaac ctataccgct tacggtagaa aagataatcg
     3961 ccgaggtagg aaagatatac agcgttaccc ccgaggatat gcgttcacag aaacgttctt
     4021 cacagataag caccgcaaga caggtggcta tctatgtagt aaacaggata acaggtcttt
     4081 catattcaaa tataggcgtg gagttcggta acagagatca ctccactata gtctatgcaa
     4141 taaataaggt aaagtccagt atcaagaaag atccgtcctt taaaggtatg atcgacgata
     4201 tgataaaaaa cctcggaaac aatgcctgat ctatgtaaat ttcaccgccg aataaagact
     4261 gcggtgattt tttacacaat aaaaatcatt caacaagttg ttagttttaa accgggaaag
     4321 caagttgaca gtgtttaatg ttgaaactgt gttaaaac
//
LOCUS       DAWAOG010000120         4339 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2606_length_4339_cov_2.035714, whole genome shotgun sequence.
ACCESSION   DAWAOG010000120 DAWAOG010000000
VERSION     DAWAOG010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2606_length_4339_cov_2.035714"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    230..283
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2100..2109
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2685..2694
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1358 a          881 c          995 g         1031 t
ORIGIN      
        1 tggtcgcaca gtcgatagca caggaaaaaa catcaaaggt aatggagctt ctgctgacct
       61 ccgtaagacc gctcgctgta attatcggca agatacttgc gatgggttca cttgcactga
      121 tacagttcct tatgatcatc gcatcgggcg cacttggaat tgttgtttcg acacctttgt
      181 catcatcgat tatcggctcg tccgaggaaa cgggagctgt aacaagtcan nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcttatg actgaagtcg
      301 gaaatgcgct tggcggattt tcgccgatcg tcatcgtgct cattattatt gtgttcatag
      361 taggatttat tttcttcgca cttatcgcgg gtcttatagg tgctacggta agcagaatgg
      421 aggatctcaa tgccgcaatg caacctatgg caataatagg cgtacttggc ttctatcttg
      481 cttatcttcc gtcttcgatg ggcggagagg caaatacgat ggcgctgata tcctactatc
      541 ttccgatttc atcaccgttt tcgatccctt ctgcactgct tacgggggcg atggatatcc
      601 cgcaggcact tttggcggta cttgttctgt gcgtgttcgc agttcttatg gcgttacttg
      661 ttgcgcgcgt gtatgagcag gtaatactgc atacaggcaa caggttgaaa attagcgata
      721 taataggact tgccaaaaac aaatagtaat tccgttttca tcagtaaagg taaatgagat
      781 gaacagtata agcagcttcc gaaaaattct atacgttata ttcaccgccg tgattttagt
      841 tacggcggta tcgctgtttg caaaacctgt cgaagcaggt gcggcagatg cagtgacatc
      901 aataagcgta acacctatac agccgacgct tgacatatcg gataagcgga cggtctatcg
      961 ctataacagc aacagtggca aatatgtttc gcatagctac tatgatgtgt acgactatga
     1021 taaaattaag cttacagtga attacggaag cgccgagaaa acttatacag gcagcgaaat
     1081 aacacgtctt acggcaacgc tcggagtacc gctgataata tctgacggac aggcagataa
     1141 agcgtggagt gacggcattc atactgttac atacaagctc ggaaatgcaa ccgcaaaggc
     1201 aacgtttgaa gtagtcgaca ccaagatagc acatcttacc gttactccga tatatacgat
     1261 aaatgcgata tacaacgttg acggaagcta tgggacattt gcggacagta cgggaaaaat
     1321 atctcagcgg tttatttatg accttggagg atatgattat aatgttaaaa taatctatcg
     1381 gggaggtgtg acagtaaact gtactgccgc cgatttaaag cagaagaccg gatatgaagc
     1441 ggtattctca cagggtaacg ccgcgttgtc ggtcggagca aacaagggct actgcactat
     1501 aggcggagta actgcatcgt ttgacttcaa tgttgtttca aatcctataa aaaatgtaca
     1561 gctttatatg acagataatg cagatgagct ttatgcaaca cttgacggtt attacgacaa
     1621 aagaaatgac ggaagtgcat atttccgatt tatatacgat cgtacaaaaa tcagagcaaa
     1681 ggtcacactt gcaagcggag aggttgcaga ttatcctata ggtgagcttt gtgcaaggct
     1741 tcacagcgaa ctgtacattg aagatcggca gagcgcttca ccgtggaaag taggaaatat
     1801 cacattgccg gcaacgatct gcggtattaa aacctctctt gcgtttaaaa taagcggagc
     1861 cgcacccgta agcgggctca tagtggaaaa tgcaagtgat aactcagtaa aaatagcgtg
     1921 gaacagcgga tacggtaccg gatatattgt cgaaaagtat gacggtaaaa agtggataac
     1981 ggtggctgag cttgacagca aggctacatc atatacgttt acgggacttg aagaatgtac
     2041 cggatatcat tttgcgatca gatcgtatta taacggcaat gcagggtcgg agtatacagn
     2101 nnnnnnnnng tatacagatt ggacatataa agattccgaa accgctatgc cgattgtgga
     2161 aagcttcaga acggcaacaa gaacgtcaaa ttctttgacg ctttcgtgga ataaaaactc
     2221 aacaacagac ggatatattg tcgaacagta taaaggcgga aaatggtcgc gtattgcaaa
     2281 aacctcatcc gcatcggcag ttgatataac tgtaaaatca ctttcggcag gaacatcata
     2341 ccgtttcagg ataaaagtct ataaaggaag taaaagcggc aactatgtgt atctttgtgc
     2401 cgatacactg ccgactgtgg taagcggctt taaacgaagt gaccgctcaa gcagtaccgt
     2461 aacactcaac tggaacagaa atacgtcggc gaccggctat gtgattcagc ggtacaaaaa
     2521 cgaaaaatgg acagatgtta ccgttattcg taacaaatcc gtaacaagct acaaggtgag
     2581 cggtcttgcg gcaagcacta cgcacaagtt cagaatctgt gcctataaaa ccacagataa
     2641 cacaacctcg tacagcggat ataaaaatat aactgccaat acatnnnnnn nnnngtatac
     2701 gaacggaaag tgggtacgct tgacaaagct gacatctgcc gataaagtaa gctgtagtat
     2761 agtgaagctg tcggcgggca cttcttacaa gttcaggata aagacgtata aattcttcgg
     2821 aaagaatgcg ttgtacagcg gatattcgac agtgacggct ttgacgacac ctaaaaatat
     2881 cggaggcctg aaatatacaa agagaaccga cagtactgtt acgcttaatt ggaacaaaaa
     2941 cagcgcagcg acaggctatg agattcaggt atataaaaac ggtaaatgga caactgcggc
     3001 aaggatcact tcttacacta aaaccggttg taccgtttca aagctgccgg cttcaactgt
     3061 gacaaaattc agagtcagag cgtataaaac agtcggcaag ctgacttcat acagcgcgtt
     3121 ctcaaatata tccgtgcgga cgttgccgtc gaatgtcgat tcgtttaagt gtgttgaaag
     3181 cacaagcagt acggcgaagc tcacatgggc taagaacaat tcggcaacag gttatattct
     3241 tgaacagtac agcaacggaa aatgggttcg catcgctaag ttcacatcag gcagtaatac
     3301 aagctgtcga attataaagc ttgcttcaaa cagagcgtat aagttcagga tatgtgcgta
     3361 taagacagtc ggtaagagtg ctctgtacag cggatacaca aagcttaccg tatattctgt
     3421 tccgacaaat gtaagctcgt ttacatcagg cggaacaaca aacagtaccg taaagctcaa
     3481 ctggagaaag aacgccactg ccgacggata tataattcag ccgtacaaaa acggtaaatg
     3541 ggtgcgaata aaaaagctca cccaaaatac gaaaatatca tataccgtta ccggacttcc
     3601 cgcaggttcg acctgcaaat tccgcataca agcctataat ttcgtcggaa aaaaagcggt
     3661 atacagcgga tttgcaagtc ttacagtgct gacaaaaccg actgaagtcg cctcgttcag
     3721 atgtgccggg aaaacaggca atgcattaac gctcaactgg aaaaagaaca gttctgcatc
     3781 gggctatata attgagcagt acaaaagcgg taaatgggta cgcatatcta agatcactaa
     3841 aaataccgct gtaagctaca ctgtcggaaa tcttaagaag aatacgactt ataatttccg
     3901 catcaaggct tacagaactg taaacaaatc gactgcttac agcgggtata agactattaa
     3961 agctacaacg tcaaaatgag aaaggtcgaa caaatggaaa taattgttgc agttgacgag
     4021 aactatggaa taggaaaaaa cggagatctt ctggctcatc tttcgcctga cctcaagcgt
     4081 cttaaggcga tgacagtcgg aaacataatc gtaatgggaa gtaagactta tatgagcttc
     4141 ccgaagcggc ctttgcctga cagggaaaat attataatca cacaccgccc cgaggaatac
     4201 cccggtatca gatgctttac atcgaccgaa gaatttgttg aatactcaaa gacggctcaa
     4261 aagccgatat ttgtactcgg cggcggaagt atatacgaac agctcttgcc ttactgcgac
     4321 aaagcgcacg ttaccagga
//
LOCUS       DAWAOG010000121         4331 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2607_length_4331_cov_2.023386, whole genome shotgun sequence.
ACCESSION   DAWAOG010000121 DAWAOG010000000
VERSION     DAWAOG010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2607_length_4331_cov_2.023386"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1353 a          923 c         1048 g         1007 t
ORIGIN      
        1 agtcgaaaat cgtcccgatt gggaaaacag ctcagagcag tttgtcctgt gcgcaccgtg
       61 gtggataaga agcggtcacg ctcttgataa atatgttgtt gtcggtcatt tcccgaccta
      121 taatttcgca agaggaaaaa acaccaatct gcctataatc gacaatgata aaaagatcat
      181 tgatatcgat ggccgctgtt cggtaaaatc ggcaggacag ataaatgctt ttataataac
      241 taaggacgga gaaggctaca gctatgacaa cgtattcgag ccgtccgagc cgtgtgaaaa
      301 atgtacggtc atcaaggatt atacagccgc tcggcattat tcttatcttg attatgaaaa
      361 gagcgacctt gaaatactcg gcaagtcaga cggactggtg tcggtgcgtg ataagcacag
      421 cggcaacagc ggacttatac ttgaaaacta tatcgcccaa tggggtgacg gacattatca
      481 cggctggtca aatcttgatg cttttttgtc cgttgataaa ggcgaaacat tctgcgttta
      541 ttataataat gaaaaatact gctacggcat agcacaaagc ggagaagtag gaatgatacc
      601 acttgactgc gttgccgtat ttaaggagaa atatgtttaa agtactgaaa acagacggag
      661 tagcaagacg aggagaattt cacacccctc acggtgtgat acagtcgccg ttttttatga
      721 atgtcggcac ttctgcggcg atcaagggcg gtatatcatc gcttgacctc aaagacctca
      781 agtgtcaggt ggagctttgc aacacctatc atcttcacgt tcgtccggga gatgatataa
      841 tatataaaat gggcggactt cacaagttta tgaactggga cagaccgata cttaccgaca
      901 gcggcggatt tcaggtgttc tcgcttgcta agctcagaaa gataaaggag gagggagtat
      961 atttcaactc ccacgttgac ggcagacgta tttttatggg tcccgaagaa agtatgagga
     1021 tacagtcgca ccttgcctcg actatagcta tggcatttga cgaatgtata gaaaatccct
     1081 cgccgtatga ttatgtaaag aagtcggtcg acagaacgac aagatggctg cagcgctgta
     1141 ttgacgaaat ggcaagactc aattctctgc ccgaaacgat aaacagacaa cagatgctgt
     1201 ttgcgataaa tcagggcgga acttatgacg atataagagc ccagcatatg aaaactattg
     1261 caaatatgaa tgttgacggt gtggctatcg gcggacttgc cgtaggcgag cctacagacg
     1321 ttatgtacag gataatcgaa accgttatcc cccacgctcc gacagacaag ccgcgttatc
     1381 ttatgggtgt cggcactccg tcaaatataa ttgaagccgt gtacagaggc gttgatatgt
     1441 tcgactgtgt tatgccgagc agaaatgcac gtcacgctac cgtgttcaca tggaacggta
     1501 tcacccacgt tacaaatcag tgctttgaaa ccgattcgtt accgatagac cccgaatgtg
     1561 tctgtcctac ctgccgcaat ttctcaagag cgtatatacg acaccttttc aagtcgggag
     1621 aaatgctcgg cggaagactt gcagtacagc ataatctgta cttctacaat acgcttatgg
     1681 aaaagataag agcggcaatc gaagagggaa catttgaaga attcagaaat cactattcgc
     1741 cgcttttagc gtcaaaactt gtctgatttt cttataatca tcacataagg gtgatataat
     1801 aacaaactat gatttaaagc gaaaaggaga acgtatgaag cttataaata taggctacgg
     1861 caacactgtt aattccgaca gggtaatagc tgctgtgagt gccgatgccg caccggtaaa
     1921 gcgtatcata tcggcggcaa aggataacaa taccgctata gatgcgactt gtggcaaaaa
     1981 gaccaaaacg gtcataataa ccgacagcaa tcatgtagtt ctgtcggcgc ttgatatatc
     2041 acatttcaca gacatcacta cagataaaaa gggagagaac gaaaatgaat aaaggattac
     2101 ctatagttat ttccgcaccg tcgggttgcg gaaaggatac gatacttgaa cagctttata
     2161 agaagaacgc aaatcttact cagtctgtat ccgctaccac aagagcaatg cgcgagggtg
     2221 agatcgacgg cgtcagctat catttcacga ctgtagaagc atttgaaaag atgatcgcaa
     2281 acggcgaagt tctcgaatat acgcagtact gcggcaacta ttacggcaca ccaaaaaaag
     2341 ccgtgaccga tatgcttgaa cagggcaagg acgtaatact caagatagaa gttgagggag
     2401 caatgaatat caaaaagata ttccccgaat gcgtacttat cttcatactc ccgccctcga
     2461 tgagcgagct tgacagaaga ctgagaaaaa gaggcacaga aaccgaagaa acgatcgccg
     2521 caagaatagc tcaggcaaga aacgagcttc aatttgcaga taaattcgat tacctcgttg
     2581 taaacggcga gcttgaaaag gcggtagatg atgttaatac cgttattctt gcagagaaga
     2641 tgaatacaaa gcgttcgctt gcgcttcttg aagaaataaa gaagtcataa tgagtgacag
     2701 ccttgataaa gctattgtat cggtagttgt cgacaaggcg ctgtatagct ttgataacga
     2761 gtttgattat tatctgccgc agggagcaac agtcaaaatc ggtcagcgcg taatagttcc
     2821 gttcggcaaa ggcggcaaaa agcgtgtcgg attagttaca ggctttaagc aaagcgcaga
     2881 ttacagcaga cttaaagagg tatactgtgt tataaatgac ggagtgatac tcggtgacga
     2941 agctatacat ttgatgcgct ggcttaagga taacacattc tgcacctatt ttgacgctgt
     3001 aaaaactatc ctccccggag gtatggcgct gaacatatca cagagatata ccctcagtga
     3061 cgatttcaga aaagctcccg acagcttttc tcttgagccc gccgagcagg cggtatccgc
     3121 aatgctcctc ggctgtaaaa gcgaccgaga gctgaatgat atgatagatt acggctttga
     3181 cgaacagcag aagaagctcg taaagcagct ttgtgaaaag tcggtgctgg cttctcttga
     3241 catcataaag cagagagtag gcagtgaaac cgagaagaac gtcaggctga ctgaaagctt
     3301 ccaaagcggt gaatatgctg acaatggcgg aaaacccctt accgcaaagc agaaaaaggt
     3361 tgccgatttt ctggaagaag cggtatctgc ttctgtgaaa gaagtatgct ataattgcgt
     3421 tgtaacaaat gccgttataa acaatatgga aaaaaacggc atagttgaat gctttgataa
     3481 tgaagtttcc cgttcgctta cagctgaagc aaaggctgta aagagcattg acgatataac
     3541 actgtcggac gaacagtccg cggtataccg tggtatgaac caacttatgg acagcggcaa
     3601 accacagtgc gcattgctga aaggcgttac cggaagcggc aagaccaccg tcttcttaaa
     3661 gctcatcgac aaggcggtaa aacagggaag gacagcgctt atgctcgtcc ccgaaatatc
     3721 gctcaccccg cagatggtgc ggaacttcac cgacctgttc ggaagcaatg ttgcggttat
     3781 ccacagcaac ctgtccctcg gtcagcgaac cgatgagtac aagcgcatcg aaaagggaga
     3841 ggcaaagata gttataggta caagaagtgc agtattcgcg ccgctcgaca acattggtat
     3901 aatcgttatc gatgaggaag gcgagcacag ctacaagtcc gaaaaatccc cgcgctatca
     3961 ggcaaggagc atagcaaagc agaggtgctt ttatcataac gcaacgctcc tcttagcgtc
     4021 ggcaactcca agccttgaaa gtgcatactt tgcttcgata ggcagataca agctgtttga
     4081 gatgaaaaag cgttacagcc agcctgttct ccccgatgtg taccttgtgg atatgaaagt
     4141 ggaggtcgaa acgggcaacc gctcaaactt cagcagagcg cttgcagacg agataagaaa
     4201 caatctccaa aggggcgagc agacaatact tctgcttaac cgcagaggtt acaacaccta
     4261 tatgaactgc atcaaatgcg gtgaagtata caagtgcccg aattgcaata ttccgcttac
     4321 ctatcacaaa g
//
LOCUS       DAWAOG010000122         4323 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2614_length_4323_cov_2.018276, whole genome shotgun sequence.
ACCESSION   DAWAOG010000122 DAWAOG010000000
VERSION     DAWAOG010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2614_length_4323_cov_2.018276"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1265 a          889 c         1001 g         1168 t
ORIGIN      
        1 atacataact gtataattgc tattatcgtt aatgacgatt atattctttg atacacctat
       61 catattagcc gccataccaa cacatcgctc ggaattagca atgagcgttt ccagcaaatc
      121 ctctcctgtc tgcctgtcat tttcggtcgc cggtacggat ttctgcgaaa gtataaaaat
      181 atccctgttg atttctttta tcattttagc gtcacctctt acgttttatt ttaccacaaa
      241 acgtaccatg gtttcaacca ccggtacaaa tcaatcgttg cacaaaatgt aacctttttg
      301 cgccgattac gatattgcta attgtgaatt tttatattat aataacttca aacacataag
      361 tcgcgtgcgc gtattaaaat cttatcatca gaaaggaaat tgtctatgcc aaatccatat
      421 ttaccatgct gggaatatat tcctgacgga gaaccgagag ttttcggtga ccgtgtatac
      481 atctacggtt cgcatgacag agcgggttca ggcgaatttt gcgactacat tctcaaatgc
      541 tggagcgctc ctgtaaacga ccttaataac tgggtatgtc acggtgttac gttccgtgta
      601 aagcccgaag gcgaccgtcc cgcggacaca gattggacac ctgatacaaa tcagcttctt
      661 tttgcacctg acgttgtgtg cagagatggc aaatattacc tatacgctta tatagtgaat
      721 gcaatcggct gtgttgcagt cagcgaccgc cccgaaggac ctttcacgct tctctccaag
      781 tacaagtata ccatatcaga cgaggtatgc tgcaacggct ggttcatcga ccccggcgta
      841 ctggttgatg atgacggcag aacctatata tactgcggct ttgagcgctc ttttatggct
      901 gaggtagaca ccgatacaat gtacacgata aaggataaca cctgtatcga acacattatc
      961 ccgataaccc ccgacaagga aaacggcttt gatgaggaag aatcactctt cttcgaggct
     1021 tgctcacccc gcaaggtcgg cgatacatat taccttatct attcacccaa gcgcggttca
     1081 cggcttgcct acgccacctc cgataagcct acaggaccgt ttaagtaccg cggatacatc
     1141 gtcgataacg gcgttgatta tcccggcggt aacgatcacg gctcgatagc tcagataaac
     1201 ggtaagtggt acatcttcta ccaccgtatg accaacggta cgataatgtc aagacgcgcc
     1261 tgcgttgaga agatcgagat acttccggac ggcactatac cgcctgttga aatgacttcg
     1321 ctcggctttg aggacagcct gaacccgtac agaataacag atgccgagat tgcctgcgta
     1381 cttaagggcg gagcgtttgt caccgagcat aacgagtttg aaagggtagt cacaggtatt
     1441 aaagacggcg ctgtattagg ctataaatac tatgatttcg ggcttgacaa cggcaacggc
     1501 agaatgtact tcaaagctga tataacaggg cttggcgctg attgcaatgt gttcaaggtc
     1561 ggtcacggca gcggcgttta caagacggag gtaaagcctg tcagcggcag acacgctgta
     1621 ttccttacag cagacacgga ttacacaggc tgggtgactt gctatttcaa ggaaaaaccg
     1681 ctgtttgaat tgaagaagtt tgtgttcaca aagtagtttt cagtcgttac gaggtgaaat
     1741 tatgaagata ggctctatcg gatataatta tgtccatgat atgaaatacg aaaattttgt
     1801 tatggacaga ccgaaaggtc cgggtgcggt cttgttgctg tttataaaaa cacctgctgt
     1861 tttcaaagtc ggcgaggtac agtatcaggt aaaagaaaat tcgttcatct tattgtcggc
     1921 ggatacaccg tgcttttata ctgcgcaagg tgatgtatac accgatgatt gggtatactt
     1981 tgaaaatgag ttctgggata aagattatgt tgaaaaactg gggataccta tggatattcc
     2041 ggtgtatctc ggagatatcg atgagctttc tcaccttgtt catatacttg tctacgagca
     2101 ttattcgggc gctgtgaaca gcgatgagat cgagaaaaag tatatagatg tactgtttct
     2161 tatgcttagc cgcacgctca ggtcgtgtaa ctgtatgtca tcgaaacagc ttagtgaaag
     2221 gcattaccgc tttacgcaga taaggacatt gatattcaca atgcctgatc aggtaggaaa
     2281 tgttgacgat ttggcggcac aggcaggtat gagtcgttcc ggctttcagc acctttataa
     2341 aaagctgttc ggaacaacgg ttataaagga tataatcgag ggtaggacgc aacgtgcgaa
     2401 acgtttgctt tcctctacaa gactaacgat aaaggatata ggtacgcgct gcggttatac
     2461 aaatgaatac agttttatgc gacagttcaa ggaacaggta ggaaaaacac ctacagagta
     2521 ccgcagctgt atataagggg aatgtttatg aagaagatat tgtctgccat attggcatta
     2581 tgtatgatag tttcggttac tgcctgttca tcggaaaatg aatcgtccgt tcccgaagaa
     2641 gtaaagggta gtgagaacag ggcaatgcgt gagatgacaa cgcaggaact tatcgaggat
     2701 atgggactcg gtataaatct cggaaatacc tttgaatcct gcggaaactg gataaacaag
     2761 tcgtctgtta ccaattatga aacagcgtgg ggaagtcctg ttattaccga ggaaatgatc
     2821 aagggctaca aggactgcgg tttcggcgtt atgcgtctgc ctgttgcgtg gagcaatatg
     2881 atggacaagg aaacgtatac cataaatccc gactatattg caagagtaaa ggaagtgctg
     2941 aactgggcgc ttgacagcga tttatatgta attctcaaca ttcattacga taacggctgg
     3001 ttttcggact ttgccgatga taaaaagaga gatgaatgct ttaagaagta tgaagctata
     3061 tggaaacagc tttgcgagga attcggcgat tacggcgacc atctgatgtt tgaatctctc
     3121 aacgaagagg gcggttggga ggaactgtgg aacagataca gcaacgaggg tgacaaggaa
     3181 aagtcgtatt ctatattgaa tgaaataaat cagaagtttg ttgatatagt aagggcttcg
     3241 ggaaagaaca acggcaagcg tcacctgctt attgcgggat ataataccga tatagatctt
     3301 acctgtgatg agctttataa gatgcccgat gaccccgaaa aacgttgcgc ggtatcggta
     3361 cactactaca caccttcaac cttcgccata cttgaaaagg acgcaagctg gggcaaggca
     3421 aagacaacct ggggcacaga aggcgacaag gaattgctta caaagtacat ggatatgctg
     3481 aaaactacct ttgtagacaa gggtatcccc gttattatag gtgaatatgg ctgtacaaca
     3541 aagaacaagc aggcaaaata cattaatcta tatctttcaa gcgtctgcaa ggaggcatat
     3601 tcgagaggta tgctccctgt gctgtgggat gtaaccgatg tattctatga ccgcacgacc
     3661 tgcacgttta aagatcccga tctgcttgcg gatctgatgg cggcaaagga gctcgaaaga
     3721 aacgtataac aaagtacaga ctgtcgataa acctacataa cttaacaaat ggggatagag
     3781 aacaagcact tttttgaaat aacagacttt ttctacaaac tgaacaaagt aattacctat
     3841 ttataaaatg actcccggag tgaaatactc cgggagcctt tttatatggt tgtttacgcc
     3901 ttcagttcaa atgacttaca gtctgtgcaa gccttctgtg tggggtctgc ctcatgtgtg
     3961 ccgatctgga tatgatcgag tgagcagtaa tccgcacagc agcagtgatg cttacagttt
     4021 gttattgaac atgcgatgca cttgtttgcg tgttctgttc ccatttttcg tacttccttt
     4081 cagaaataaa atatgagtaa agttgtcttt acgcaaatta ttataaccgg aaagccgaaa
     4141 aatacaacag aaacatcatt ccattattat gtttaaaata tcggaaaatt caggcacgat
     4201 ataattcgct ttatattctc tcagctcttt ttccgagccg aagccacaca gcactccgac
     4261 cgattttatg ccacattgct ttgcaccgat gatatcgaat tttctgtcgc caatcatgat
     4321 gac
//
LOCUS       DAWAOG010000123         4310 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2627_length_4310_cov_2.378378, whole genome shotgun sequence.
ACCESSION   DAWAOG010000123 DAWAOG010000000
VERSION     DAWAOG010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2627_length_4310_cov_2.378378"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    251..260
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1240 a          928 c         1092 g         1040 t
ORIGIN      
        1 cagtatacaa gatggttgat acctgtgccg ccgagttctc agcagaaact ccttatttct
       61 acagcacata cgatgaagaa aacgaggctg ccgaatttat cgagcagcac ccgacagata
      121 aaaagaagat aatagttttc ggctcaggtc ctatcagaat aggtcagggt atcgagttcg
      181 actactgctc ggtacactgc gtatgggcgc tcaaggaaat gggctatgaa gcaattatat
      241 gcaacaacaa nnnnnnnnnn aaacagtttc caccgacttt gatactggcg acagacttta
      301 ttttgatccg ctgacatttg aagatgttga ctcgctgata gctaccgaaa agccttacgg
      361 cgttgtagta cagttcggcg gtcagactgc tataaagctt acaaagcact tacaggagag
      421 cggagtaaga atactcggta cttcggctga aagcattgat gacgcagagg acagagagaa
      481 gtttgacgag ctgcttgaaa agtgtaaaat tcctcgtcct aagggacata cggtattcac
      541 gacagaggaa gcactcaaag ccgcaaatga actcggctat cccgttctgc tccgtccgtc
      601 atacgttctc ggcggtcaga atatgataat cgctcactgc gacagcgatg ttaccgaata
      661 tatgactatt atcacagcta cagagcttga aaaccctgta ctgatagata agtaccttat
      721 gggtactgag gtcgaggtcg atgctatctg tgacggtaca gacttcctta tccccggtat
      781 tatggagcat attgagcgtg ccggagttca ctcgggcgac agtatctcgg tatatcctgc
      841 acagacactg tccgacaaga taaaggaaac tatcgtaaca tatacggagc gtcttgcaaa
      901 ggcgcttaac gtaatcggtc ttgtcaatat acagtacgtt gtatataaca acgaggttta
      961 cgttatagag gtaaatccca ggtcttcaag aacagttcct tatatcagca aggtaacggg
     1021 cgttcagatg gtcgatatcg caacaaagat tatgctcggc gaaactctta aggagcaggg
     1081 ttatcacagc ggtctgtatc ccgtaggcga ctatgtagca gtcaaagtgc cggtattcag
     1141 ctttgaaaag ctccacgatg ttgatacaca gctcggtcct gagatgaagt caacgggcga
     1201 ggcactcggt atagcaaaga catttgaaga ggctctctac aagggactta tagcggcagg
     1261 cttcaagata ttcaagaagg gcggcgtaat gttcaccgtt aaaaattccg acaagcccga
     1321 aattataccg cttgccgaga agtttgaaaa gctcggcttt gagctgtacg ctacaagcgg
     1381 cacggcttca atgctgaaca gaaatatgat agctaccaac ttcacatacc gtatacacga
     1441 ggacaaagag ccgaatgtta tcagtctgct tgaaagcggc aagataaatt acgttatctc
     1501 aacctccgaa acaggcagaa agcctgctct cgacagcgtt aagataagac gtaagtcggt
     1561 cgagcgttct atagcctgcc ttacatcggt cgatacggcg aacgctcttg taaagtgtat
     1621 cgctatggac aagactgtag acgatctcga aatggtagat ataacaaaga tttaatgagg
     1681 tgcgatattg aaaacaggcg aatataaagt aaaattcaat tacacgatat tgaggggatt
     1741 tatagacgct ttcggtctgg ttatggctct gctcatatat cagacgacat acgcatacta
     1801 tgataagttt atccaccccg atcaggtgat aaacaatgtt tatccgaacg gtgtgccgct
     1861 tgacagatgg atactcggcg gcatagttcc gtttatcgct cttgcggtgc ttgtgttctc
     1921 ggttgtgtat atgtttatcc cgcataagtc aagaacgctt atgataacag cggacaatgc
     1981 acagaagtat tacgacaacc tgatgatagc aaacagtctg ctgagaatac ttgcgcttat
     2041 ggcactgtgg gattacactt atatttcgca gagcaacctg ctgtatgcgg gggagtcgtg
     2101 gttcagtctt cagacggtgc ttgacattgc ggtcggagcg cttgtcgtat actttatgta
     2161 cggcagactt aaagctatag caaagcctgt cgataaaaag aaaaaggcgg aaaggaaatc
     2221 tgccgccggc gaagaaacaa cgcttgcaaa ggtgaaaata gtcaaggacg acgatacaat
     2281 aatgcgcagt tagctatgca aaaggcaaca caatagaaat aacgtgttgc cttgattttt
     2341 acacagcggt aataccgcag gaaaggaaaa tatatgagct ttttttgtaa ttcatatccg
     2401 atagttgata aaaaagcgct cggcgagggt atatattcat atacgctgaa atgccccgag
     2461 gtcgctgaaa acgcacatat cggacagttt gttcagataa aggctgaggg ctatatgctc
     2521 cgcagaccca tatctatatg ctcggtcgac aaggaaaacg gcacaatgcg ccttgtattt
     2581 gaagtaaggg gcaagggtac ggataagata tccgagctta acaagggcga tatgatggac
     2641 gttatcgcac cgctcggcaa cggctttaca gttcccgcaa agaatgacgg cagagtgata
     2701 gtagtcggcg gcggaatagg cacaccgcct ctgcttgata ttgcaaagct gtatggcgac
     2761 aagtgcacgg caatacttgg cttcagaagc ttcgacaagg tgatcctcga caaggactat
     2821 gccaaagcag gcgcaagcgt tatagtctgc accgatgacg gcagtacagg tgtacacggt
     2881 acgatagcac agccgcttgc agatgagctt gccaagggcg atgttgcggc ggtatacgcc
     2941 tgcggtccta ctcctatgct gaaagcggtc gttgcggcgg caaagcaggc aggcgtatat
     3001 tctgaggttt ctcttgaaca gcgtatgggc tgcggtgtcg gtgcgtgtgt tgtatgtgcc
     3061 tgcacggtta tccgtgacgg agaagagaag gtcgtaagag tatgcaagga cggtcctgtg
     3121 ttcagcggtg aggaggtagt attatgagtt tcaagcccga tatttcagta gatgtatgcg
     3181 gcaagcattt taaaaaccct gtaatagccg cttccggtgc atacggcttc ggcgaggatt
     3241 acactgacct ttatccgctg tcggcgctcg gcggtatttc ctgcaagggc acgaccttaa
     3301 acaagaagga cggcaacatt cctccgagaa tagccgaaac tccgagcgga atattaaact
     3361 ccgtaggact tcagaacccc ggtgttgata agtttataaa ctactatctg ccgcggctca
     3421 gaacgcagga tacggtggtt atcgcaaata tcgcaggtgc tgtaattgac gattatatag
     3481 ctgttgcaga aaagcttgat gctaccgatg tcgatatgat agagcttaac atttcctgcc
     3541 ctaacgtaaa gcagggcggc gctacatggg gcgttacctg cgagggtgcg gcaagcgtta
     3601 caagagcggt aagaaaggct acaaaaaagc ccgttatcgt aaagcttact cccaatgtaa
     3661 caaatatcac agaaatcgca aaggctgttg aagcagaggg tgctgacagc gtttcgctga
     3721 taaacactct gctcggtatg agaattgata taagaacaag acgacctatc ctgcacaata
     3781 atgtcggcgg actttcaggt cctgctgtgt tccctgttgc ggtaagaatg gtatggcagg
     3841 tatcaaatgc ggtaaagata cccgttatcg gtatgggagg catcgcaacg gctgaggacg
     3901 ctattgagat gatgatggca ggcgcatctg ctgtacagat gggtacggct atcttcaacg
     3961 acccatatgc accgataaag gtgtgcgagg gtatggaaaa gttcctcacc gaacagaaaa
     4021 tagagaagat aagcgatatt gtaggcacgg taaagccgtg gtaataagct tcacagaaga
     4081 atacaaacag caatgccccc acaggtttca ttcctgcgag ggcatttcag ttattcctta
     4141 tttataaact tatctatgat gtacttaggc cttgccttga cctcggtata gatctttccg
     4201 atatattcac cgacaattcc cagcgccatt atctgcagac cgcttaaaaa ccatattgag
     4261 cacataagcg agctccagcc tgcttctgtg ttgccaagac aggtctgcac
//
LOCUS       DAWAOG010000124         4297 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2638_length_4297_cov_1.892504, whole genome shotgun sequence.
ACCESSION   DAWAOG010000124 DAWAOG010000000
VERSION     DAWAOG010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2638_length_4297_cov_1.892504"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1237 a          934 c         1021 g         1105 t
ORIGIN      
        1 tttatccgta agatatcgct gcaggtcgaa attgaacccg tctgctttgg cggaggtaag
       61 tatgtccgat gtatcatcgc cctgcatacc ctcccagtac cagttgttga attcatcgtc
      121 gggatctttc ggctgagaat acactatcag ccagtgtttt tcgtcataga atcggtttac
      181 ataaagctca taagcgtact tctcgaggtc ggtatagtta tcctcccagt cggagttgta
      241 cacagtcatt accgcaggag aaatacccgt tgtgttgaag aaatcctcaa gtgcttcact
      301 gagctttgtt gtatctccga gtacgttagc atcgtcctgt ataactatct gcgagttata
      361 atcaaccttc agcttttcgg gtacgccgaa agacgtagat atcatcaaga atgccgccag
      421 aacaaacggt acatagaata tgagcattaa aaatctcagc ttgctcggac cttttgataa
      481 atcggtatcc gagtagacgt aattcggaac gccgtttctg tagtagacga atcttcttgc
      541 accggggaaa tatgtacggc tggttcttat tctgcttgag ccgcctcccg atgatgaacg
      601 gtgtgacgag ctgtgcgatg aggagtggtg gcttccgccg ccgtgagagc ctccgcccga
      661 tgagtgtggc atattatacc ttctttcgtg tgacttttga tattattcta ccataaatta
      721 accgtcaaat caagatataa agctattcgg agtgacggct gaaaactgta aagcgttaaa
      781 aatcaaatgt aaagtcgcac ggcaaaaatg taaagtctgt gtatagtttg gctttgctat
      841 gcgaaataca cagttttaca aaagctttac aaactttata ccgttccttg ctttaatgta
      901 aagcccattg cgactataat atgaaatgta gtcgggaaga aaaccgaacg acagataaaa
      961 atgtatataa atattacgga gtatcaggaa aactccgagg gagaacccct tcaagaaagg
     1021 aaaacaacat gaacaagaag ttaatatcac ttatcgcagg cgtagcatta gcagctacag
     1081 caacaggctg ttcagacagc gcaacaagca gtacagccgg cacatcttcc gcagcaggca
     1141 catctgcaag cgaagcgtca tcggcagaag catcagctac agaagaactc agcggtacac
     1201 tgtcaatgaa cggctcaaca tctatggaaa aggttatcaa ggctgtcaac ggcgctttca
     1261 tggaaaagaa caagggcgta acagttaact taaacctcac aggttcaggt acaggtatcc
     1321 aggaagcctc agagggcaag tgtgacatcg gtaactcgtc aagaaagctc aaggatgaag
     1381 aagctgaaaa gcttgacgct acagtagtag gtcttgacgg tatcgctctt gtagttaatc
     1441 ctacaaacaa gcttgaggat atcacaatag aggatctcgc aaaggtttac tcgggtgaaa
     1501 ttacaaactg gaaggatctc ggcggcgatg acaaggctat cgtagttatc ggtcgtgagg
     1561 acggttccgg cacaagagat ggttttgagt cgatcgtaat gggtgacaaa gagcctaagt
     1621 acgcacagga gcttgaatct acaggttcag ttatcaatgc ggttgcaaca acagacggtg
     1681 ctatcggtta cgcttcactt gcaaacgttg atgaaacagt aaaggcactt aaggtcggcg
     1741 gcgttgaagc ttcagaagag aacgtaaaga gcggcactta tgaagtacaa agacctttca
     1801 tctgcgcaac actcaagggt tccgataaca agcttgcaaa agcttacctt gacttcattc
     1861 tctcagaaga aggtcaggca ctcgtacttg cacagggtgc agttcccgta aagtaaaaat
     1921 caagttgata attcctatac agtaaatggc ttttccggta gcaatccgtt accggaattt
     1981 gctgtataag gcatattgta tgtgatcccg aaaggatggt ttacttggaa aaaatgagcg
     2041 gtgcagccgt tgcggtgagc acgataggaa aacagagatc tgcgatcaga agtaaaaaat
     2101 caggatttac gaatgtaacg gaacgtgtga tgaaatacat cttcactgta tgtggcataa
     2161 ctgctgtagc gtgcgttctg atgataacga tatatatgat aatatccggt gcgcccgcta
     2221 tcggaaagat cggtttgacc gactttttgt tcggcgacac ctggtatgca aagaaagaac
     2281 agttcggaat acttccgctg atactcacgt caatattcgg tactctcggt gcaatagtga
     2341 tcggtgttcc gataggactt ctcacagcgg tgttcatgtc cgagcttgca cctaaggcta
     2401 tgaagaacgt agtaaagcct gcggtcgagc ttcttgcggg tatcccgtcg gttatatacg
     2461 gtcttctcgg tgctaacctt gtaaaaccgc ttatgtatgc gctggaggag gtcatttatg
     2521 ccaacgaccc cgagcatcag ttcacaggcg gcgcaaacct tgcgagtgct ataatcgtgc
     2581 ttgcgataat gatactcccg acaattatca atatctcaga aaacgcaata agtgcagtac
     2641 cctcagagta cagagaggca agctacggac tgggagctac caagatacag actatcttca
     2701 aggtagttat ccccgccgca aagtcgggca ttgcgtccgg tgtcgtcctc ggtgtcggca
     2761 gggcaatagg cgaggcaatg gcgatcatcc ttgttgcggg caacagtgct aatatgcccg
     2821 agctgttctc atcggtcaag ttccttacaa caggtatcgt tgccgaattt ccgtattcaa
     2881 gcggacttca cagagaagca ctctttgcga tcggacttgt gctgttcgta ttcattatga
     2941 taatcaatct tattttaaac ggcatactca aaaagggcgg caagaaggaa aaagcggcta
     3001 agaccgcagg aaaggcgtga tcggttatgg caaagacagt ttccaattca cttacctcaa
     3061 agcgcagacg accgagcgat ataatactta agctgttgat attcctgtca tcgggcatag
     3121 ttgtagcgct gacgcttggt atgataatct acatagttat aaagggtacg gagggtatga
     3181 gcctgaactt cctcactact ccagcatttt catatcctta tgaaaattac ggtgtgctcg
     3241 gtaatatagt caatacgctt tatctcgttc tgataacact tatagtcgca acgcctatcg
     3301 gtgtaggtgc ggctatatat cttaccgaat atgcaaagca gggcagactc acaagaataa
     3361 tcgaatttac caccgaaact cttgcaggta tcccctcaat tatatacgga cttttcggca
     3421 gcgtgttctt ctacaatatc ttcaaggtaa actactcact tctgacaggt gcgttgacgc
     3481 tttccattat ggttcttcct acaattatca gaactacaca ggaagcgata aagacagtcc
     3541 ctatgggcta cagggagggt gcaataggtc ttggcgcgcc aaagtggcac acaataagaa
     3601 ctgtcgttct cccgagctgt atcgatggta tcctgacctc tgttatcctg tctatcggtc
     3661 gtatagtcgg cgaatctgcg gctcttatct tcacggcagg tattgcggct gaggtcggta
     3721 tcaactcgat cggcgatatt ttcggcaagg tgatgaatca gggcggttcg ctcacggttc
     3781 agctttatca atacgcacag cgcggcggtg ccgaaatgaa gtacgcattc tctacagcgc
     3841 ttgtactgct tgtgacagtg cttataatca atatctgtgc aaagcttgta gcctacagaa
     3901 ttagaaagaa acgttcggta taaatccgta aatcaagaaa agaggataaa caatgtcaga
     3961 tataatcgta cctcagagcg atgtcaagct ctgtacaaga aatgtaaatc tctattacgg
     4021 cgaaaaccat gcgctgaagg atataaacct tgatataaag acaaacaaga taaccgcctt
     4081 catcggtcct tcgggctgcg gtaagtctac atatcttaaa agccttaacc gtatgaatga
     4141 ccttgtgcct gactgtaaga tcacaggtca gttcctgctt gacggtgagg atatctatgc
     4201 ggacggcgtt gatacaaacg tactgcgccg caggatagga atggtgttcc agaagccaaa
     4261 tcctttcccg atgagcattt acgataatat agcatac
//
LOCUS       DAWAOG010000125         4295 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2641_length_4295_cov_2.034434, whole genome shotgun sequence.
ACCESSION   DAWAOG010000125 DAWAOG010000000
VERSION     DAWAOG010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2641_length_4295_cov_2.034434"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1629..1638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2754..2763
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1276 a          852 c          995 g         1152 t
ORIGIN      
        1 gtcatacggt aaatgttgcg gtaacagacc tgtacttacc gtatttgaaa taaggagtat
       61 attatgctta cagtagacag atttgaagaa gataaggctg tgatctttga tgatgacagg
      121 cagatgataa taggcagaga taagctttct cccgatgcca aagagggtga tgcagtcatc
      181 ctttcggata gcggtatata tgttcccgat aaagcgaaaa cggagcagat gagaaacgat
      241 aatctcggtc ttctgcaaaa actgctgaat aagtgacaac agccctgcgg caaatgtact
      301 gttgcagggt tgttcttgtg ttagaagctt gtatgtactt cggtatagcc tccgcttttc
      361 ccaagtctta aagactgacc gttatcaagc gtcagctttg catttgacgt tttgccgtca
      421 agctgttttc gtacagaagc gataaattca tcggtacttt ccgatttgca cagaacattt
      481 ataagcgctt caagcgttga aatatctttt tcgttttcta tgaacatttc aacttcggat
      541 ttgtcaatat ttccgctttt gtcatatacg attcgaagct ttgatgtacg gctgttgtcg
      601 gctgtggatt tagtgtaaag atatacgatg ttgctgattt cctgcattgt aaagccgttt
      661 tcggtgagta tcttttcaac tgtagcgttg ctttgggaga tattgccgtt tacctcgtca
      721 tttttgccgt agcctataca caaatcggtt atctcgagtg ccagaaagat tgcaaatact
      781 aaagccgctc ttttgataac cctgcttgca acagatttct ttaccgtatt ttcggcaatg
      841 cgtaaatcag gcatactgtc gtagattttc agtattttta ttgttctgct gttactgctg
      901 tatttgagat aacgctttgt ttcgcctgtc aggacagcgt tttcgtaaat cgtcttttta
      961 tatctatcag tatcaaaatt cgctctcaca aattcaaaat ccgtatttgc ggtgattttc
     1021 gagataagac cgcctcttac ggcaagagtg cctttgatta ttgtattgcc gtcgaactta
     1081 tatgacgttg taagaactga ggcaaatctc agcagaccga taaagaatat gacgattgcc
     1141 gccgcaaaga acagcataag aacgattacc gtattatcct gcaaaaatgt gcttgttatt
     1201 ttaagcaccg aagcaccgca cagaccgata gcggtaacgc ctgtgccgac agtgaacata
     1261 gaaataaggc tcaccgccca ccaaagcatc gcaccgaatg cgtgagtgtg cttctctgtt
     1321 tcaaaagacg ggtttgagta tatcataagt ttctccgttt catatttatt tcttatttta
     1381 gtataacagc tatttccgat gctgtcaatc ggaacaattg ccgtgttcaa aattttccga
     1441 tgccgttcaa aactctttac aagtgcttga aataggtgta aaatagtatc atgatatgaa
     1501 aggagctgca ttatgaaaaa gtgcttaatt gtagtggact atcagaatga ttttgtaagc
     1561 ggttcactcg gctttgaaaa agcaaaaacg cttgaactcg gaattgcaaa taagatcaat
     1621 tcttaccann nnnnnnnnat gagggaaagc atctgcccgt acctcactgt atagagaata
     1681 cggatggata taagctgtac ggcagtgttg ccgactgcat ttccggcgaa gatattgtat
     1741 tcaggaaaac caccttcggc tcggacgctc tttatgagta tcttaaaacc gcctgctacg
     1801 gctctgttga acttgtcggc gttgtaacaa atatctgtgt tgtatcaaac gctgtgcttg
     1861 caaaaacagc attgcccgat gccgatataa tcgttgacag tgcgctaact gcatcaaatg
     1921 atgagggact gcataaagcg gctcttgata cgatggagag tatgcagata attataaaat
     1981 aaaggggcaa aagaaaatga acaaggataa tttcacaatg ctgtgtgact tctatgagct
     2041 tactatggcg aacggctatt ttaaaaacgg cttctataag cgcacaacct attttgatgt
     2101 gtttttcaga aacgtacccg acaacggcgg atttgcgatt gcggcaggac ttgaccagat
     2161 aatcgaatat ataaaagagc ttcatttttc tgccgaagat atagaatttt tgcgtagcaa
     2221 gaagattttc gatgaagaat ttctcgaata tctcaaagac ttcagattta ccggcgatat
     2281 ttacgccgta cctgagggta cgcctgtttt cccgcacgag cctgttctta ccgtaaaagc
     2341 accggctatc gaggcacagc ttatagaaac ctatatcctg cttgcgataa atcaccagtc
     2401 gcttatagcc acaaaagcga acagaatagt cagagcggca aacggcagaa cggtgcttga
     2461 attcggctca agaagggcac agggcgcaga cggtgccgtg ataggcgcaa gagcggctta
     2521 tataggcggt tgtgcaggca ctgcgtgtac gctgaccgat ttcaagtacg gtgttcccgc
     2581 aggcggtact atggcgcatt cgtgggtaca gatgttcgat accgagtatg acgcattcag
     2641 gacttattgc gaaatatatc ccgataatgc cgtactgctt gttgacactt acaacacact
     2701 gaaaagcggt gttccgaacg ctataaaggc atttaaagag gtgcttgtgc ctannnnnnn
     2761 nnnggcttga ttcgggcgat atatcatatc tgtccaaaag agcaagaaag atgcttgatg
     2821 aggcaggact tcagtgctgt aagatagttg cgtcaaattc gctcgatgaa tatcttatcc
     2881 gtgatcttat gatgcaggag gctaagatag ataccttcgg agtaggagaa aggctcatca
     2941 ccgcaaagag cgcacccgtg ttcggcggtg tttataagct ggcggctgtg gaggatgaac
     3001 agggaaacat cattcccaag ataaaaataa gcgaaaacac cgcaaagata accaatcccc
     3061 attacaagaa gctgtaccgt ttctatgaca atgaaagcgg caaggcactt gcggacgagc
     3121 tttgtgtata cgatgaaaca attgacagca caaagcctca cactattttc gatcccgatg
     3181 caatatggaa gacaaagacg cttacagact acacagcaag agaactgcac gttacggtat
     3241 tcaaaaacgg cgagcttgtt tataaacagc cgtcgctgta tgaaatcagg atttactgtg
     3301 ccgaacagtt ggaaacactc tgggacgagg taaagcgttt cgaaaatccg catacttact
     3361 atgtggattt gtcaaagaag ctgtgggata taaaaaacgc attactagaa aagtcaaagg
     3421 acggaaaaga tttaaaatga aaaaatcaat atcaataact ctctgtgcgg cattactcgc
     3481 tctgtcggga tgctcgcaaa acacaagttc agacgcttta tcctctgata tttcttctgc
     3541 cgcatcggaa tcgcaaagca cagcatcgca ggaagaaagc agcggatcat cattacagaa
     3601 tggtaacggc ggattcaaag tagaaggtac aaagctgctt gacgcaaacg gcaaggagtt
     3661 tataatgcgt ggtataaatc acgcacacac atggtatctt gatgaagata caacggctat
     3721 taaagcaata gccgaaacag gctcaaatgt agtgcgtgtt gtttgctcag acggcgagca
     3781 gtggactaaa gatacagagg atatgcttga aacagttatc gatctctgta tcgataatga
     3841 gatgattgca gttgtcgaag tacacgatgc aaccggcaag gatgataaga ctgcacttga
     3901 taaagctact gattactgga tagagatgaa gaacgctctt atcggcaagg agcagtatgt
     3961 tatactcaat atcgcaaacg agtggacagg cggctggaac ggcgaattat ggcgtgatgg
     4021 ctatacagaa tctatcccga agctgagaga agcaggtata aagaatacta tccttgtcga
     4081 tgcggcaggc tgggcgcaat atgccaagag cataggcgat tacggtaaag aggtatttga
     4141 ttccgatccc gataagaata cgatgttcgc cgttcatatg tacggcacgg caggaaaaaa
     4201 cagctcagtt atagaaaaga atctcagatt tgctaccgat aacggacttt gcgtaatagt
     4261 aggcgagttc ggctatacgc atacagacgg cgatg
//
LOCUS       DAWAOG010000126         4267 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2666_length_4267_cov_2.332146, whole genome shotgun sequence.
ACCESSION   DAWAOG010000126 DAWAOG010000000
VERSION     DAWAOG010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2666_length_4267_cov_2.332146"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2084..2093
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1026 a         1092 c          931 g         1208 t
ORIGIN      
        1 tcaaccgctt cccacagtct tatactgcaa tgaagaacgc cgttcttctt atacagcata
       61 ccaagacgat ggcaacgctc agtgttgtca acgacctgaa ctatgggcgt gatttcaggg
      121 tcatcatcaa gtatatcagc cttgctgtgc gttatcgcct cgtaccactg tggagttgtg
      181 aacttttcgg acgggaaatc atttaaaagc ttgttgtcct tatcgataag aagtcccatc
      241 gttccgggcg ctacaggctt gttcatactc tccgaaatga cacggaacat atgatagttc
      301 cagaaatcgg cgcagtatgc gttttcgata gctttactgc cgtctgttat gaccatagcc
      361 tttttgccgc ttgcgataag ctcttttgcc ttttctgtac tgccaacaaa tatcagctca
      421 tcgtctgccg tctttatgcc gttttcggta atctcaacat tgcagtcggg atagagccgg
      481 aatacatagc tgttgtgaag ctccccgaga gtaagtgtga gaactgctgt ctgcggcttt
      541 gtaataccgc tgaaatcggg agtaaatgtg ccgacagaag taagacgcgt atcggaagct
      601 gtgaactctg cactgccgct tatatcaagc tcatcgctta tgagtgaata cttaactgct
      661 ccgctcttta tcttattctt gccataggac gaaaggagaa tttctataac cggcttttcc
      721 tttgccccgt aaacatattt gtcaaatctt gcaagcagga cattgctgtc gcagaagccg
      781 cgccagtcct caggcgttat aaagcccttt gactgcataa aggcgttgag cacaccgaca
      841 agcgatacac cctgaccgtt gaagtcctgt aggtcgagaa gctggaagcc gctgagttcg
      901 gaagaacgca acgccgtttc tatctcgtcc ttatagcact gcgtacaatg tgcgccgacc
      961 gcctcaaaga aatctctcca ctgtgtataa agtccctttc cttcaagcct ttctctgaaa
     1021 atatcaagat aacgcggttt gagcgagcct gtgtacatct tcatctcgtc aaagtcaggg
     1081 aagtaatcat actgtccgac ctcgtgtgag atcacgggct tgtgcggaat atagcttccg
     1141 tctgcggcgt taagcttgac ggtctttacg cctgtaccgt actgtatctg tatcgtaccg
     1201 ccgccctcgt tctccgagcc gttctcaccg cggattattg cgtcatagtc gtgaacggta
     1261 ttaggcttgt cggtctggat atgtccgaga ggtgcatcac acatagcata cgaccctctg
     1321 aaaagtctgt cacgcgaaag tcttacgcct gcgaagaagt cctcctgtgg aatttcggca
     1381 ggccagaact ggaagttgtt tgaaccgctc gtgaaataga tcgaatgatt ttcacggcga
     1441 agaatatcaa tgatctctcc aagtctttca cgactgcccc agagctcgtt tccaagcgac
     1501 atcataacaa atgaagggtg atgtgaaaac tcccttatta tgcggatacc ctcgtttatg
     1561 aggtagttct gctcctcctc attgtagccc tcctcgccct ttgcggcgat cgttccccag
     1621 aaaggaagct caggctccat atatatgccg agctcatctg cggctgtaaa tgcggcatcg
     1681 ggaggacagc aggtgtggaa gcgatagtgg tttataccat agctcacagc gattttcagg
     1741 cgctccttcc actcattcac atcgcacgga gcgtaacctg tgagcgggaa tataagtccg
     1801 tcatgctttc ctcgcaggaa cgattcgctt ccgtttacaa gcagctttct gtcgatatgc
     1861 gaaaatgtga caaatccgaa tgtagtgttg tagatatcct cgccgcactg caaagtcagg
     1921 cggtaaacat tgcgttcaaa ctcgtcccag agctgagcgt tttcaagctt gaactgagct
     1981 tcaaacgtat cgccgtcagc gattatactt gcctttaaca cgcgtattcc gtcaggcgct
     2041 gtaacggtcg ccataatgtc ggcgttatct gcgccgataa cacnnnnnnn nnncgataac
     2101 ccttacatcg gaaatatagc tcttaggata gatctcaata tacatctttc cgacaatacc
     2161 gagccagttt gtctgcgtat cggggcttgt catatgaccg ccgcctgtct tatagccgac
     2221 atttgcaata cggataacta tctcgtgtgt gcctgcggtc atatatgaag aaatgtcata
     2281 gcggtgcgta ctgcaaagac tgtcgcaagt acccacctct gcaccgtcta tgtacagtgt
     2341 gctgactctt gtgcgttcca gcgttagcac cgatacaaag cctgtcatat cgttctcaag
     2401 cgtaagcgaa cgcctgaacc acgcatagcc tgtaaaggtg tatttgtctg tgaggtatcc
     2461 ctcgtccttg ttcttgttct cagccacctt tgcggcattt gaaagcgtat cgggcaaagt
     2521 tatactgtca ctgtaatctg tcggtattga agaaagctct ttttcgctta taaaaagctc
     2581 ccactttccg cttatatcaa tcgtatttat catagatgtt ttcctttcgt gtgaacgttt
     2641 acattctttg ctaataatac tataaagatc ttcctgtgtc aatagacgat cgggaagaaa
     2701 atattaaata tagtcgtgta aagcaagaaa aagcccgttc aatttaaatt cagtttaaaa
     2761 aaggctaata taatactggt acagggtgaa caaagcccta tagatgttgc tatcttcgtt
     2821 ttcatatatt tccccaaaac aacggtgtcc ggctgtaaaa ggtctgacac cgttgtttta
     2881 tattcgcttt actcagaata gtcaacccac atctcggtaa catcgccgtc gccgttatgc
     2941 tctgcataga tatcacccat aagcatcgca taggtatagt caccgcactc ccatacctcg
     3001 cggtagcgct cgcgaacctc cataactatg ccttttccga gcgcctcggc tatgtagata
     3061 ttgttctcgt caaagcctgc tattatcgca atatgaccgt cgcagcctat aagatcgccg
     3121 acctgcaata ttccgcttcg cattacatcc tcgtcaagcc atacacgttt tccgaaatcg
     3181 gaatattcat agtcataatc gggcgtatcc cccgcgccga catcacccag cggtacgccg
     3241 ccgttataca gacaccacga aacaaatccc gaacaggtca ttccgtttgc atactttgcg
     3301 ccgggaacaa gcccgaatga gtcctcccag tttgtgagcg gttcgcccca cgttgcaggt
     3361 ccgtacaata tacccttttt gtcaagaagc tcaaacttgc tgtgagtaag ataaagaccc
     3421 ttgtgataaa atctgccctc gccgtcacaa tacggtcttt cgggctgtgg gtcaagtctg
     3481 ccgttttcat aaaaatacgg tatcttgtaa ttaaattcaa gcggaataaa tcttgccgcc
     3541 gctactactc cggctctcgt gccgtaacct gcggtattaa cgcggctttc aagcaccgca
     3601 tcgatcagcg cgccctcctc ctcggtgtac tgtcctgccg gtaaaagcgg cttataagct
     3661 gttattttag gtgctctgag caagtctgta acggtaattt ccacactgtc cgaaagtccg
     3721 ctcgcagtag ttgccgttac agtcgctttg cccgctttca gtgcggagat cactccgtca
     3781 tttacgctga cggtttgcgg cgcggagctt ttccatgtga ccgccttttc tttatcaccg
     3841 ggtttgccta cagtatccac ctgcgcgcac agcgaataat tcattccgac attcatatat
     3901 gtatcggtct tgcctatttc gactgaggtt accgcatcga cttttaccgt tatcttgtct
     3961 ctctttgcaa gctcaccgct gtcatttgtg acataaacgt aatactctcc cccggaaaga
     4021 tcagcttctg cttctccgtc agtcgttgtg ctccaattgt ccgtatcaac gatatctgtc
     4081 tgttctttat cggaaatata ataacgcagt ccgttttcag tgtcatcgtt atttttgcat
     4141 ttaaatttta cggtacgggt aaaatcatca ttgatagtgt cggatatatc gacggatact
     4201 ataacaggga gcggcagtga aagcggctct ttttcttctg cggactgtgt tgcagtcaac
     4261 tgcggcg
//
LOCUS       DAWAOG010000127         4251 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2677_length_4251_cov_2.723546, whole genome shotgun sequence.
ACCESSION   DAWAOG010000127 DAWAOG010000000
VERSION     DAWAOG010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2677_length_4251_cov_2.723546"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2472..2481
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1292 a          847 c         1162 g          940 t
ORIGIN      
        1 gatatgccga tagcgacgat atcataaacg aaggcgttat agcgcttatg gcggctatag
       61 acggctttga tcccgataag aacgtgaagt tcgagaccta tgccggcata aggataaagg
      121 gcgcggtcat cgattatatg agaaagctcg acagagtgcc gagaacgctc cgcaagctgt
      181 caaagcagct ggacgataac tttgcaaggc tgaactcaga gctttgcaga gcgccgacag
      241 atgaggagct tgcgcgaagt atgaatatga ctaccgcaca gctgtcaaag cttatggcaa
      301 atacggcggg aatgataacg ctgtcgtttc aggagctttt atacgaggac aatcttgata
      361 aaaatatgtc ggcaaccggt gaaactgccg attcaaaaat atatgagcgt gagaaaaaac
      421 aggtgatagc agatgcggtc gccgctcttc ccgaaaagga aaagcaggta gtcacgatgt
      481 actattacga gaagctgaaa tactcggaaa tagctaaggt catgggtatt acgcagtcga
      541 gagtgtgcca gatacactca aaggctatgc tgacgctcaa aaacaagctg gaaagctata
      601 taaacagctg aaaagcttgc atatttatgt gaaaactgat attatataat caaggggcgg
      661 agcacttctc tgctctgccc gataatatgg atacaccgat ttgagaaagg aggtgaaaaa
      721 acttgcagag agcattttac aatctggcgc agtcgatgat aaaccatcag aggtcgctgg
      781 acgctatctc gaacaatctg actaacataa acacatcggg atataaaaag gacgagataa
      841 ggaccaacac gttccaggag gagcttatac ttgtcagcaa ccgcagaaag acaagcggca
      901 agttcgtaca gacctatgtc gatacgagca agacaaacct tgaacagagc agctttgaat
      961 ttaccgagag tccgtttgat ataggtattc agggtaatgt atacttcaat atacaggata
     1021 cgaacggaaa tgtatatcag acaaggaacg gtcagtttga gcttgacgga gagggttatc
     1081 tgtgtcttaa cgacagcgga cgtgtattag gtcagaacgg cgagatccat atcggaaacg
     1141 atgatttcat agttgacaac gagggtaata tcttaaacga gaacgggcag gtaatcgaaa
     1201 agctgaggct cagctacata cccgaaaacg cagatgttac aaaggtcgga aacgatttat
     1261 tctcgtatga cggagatatg acaattcccg caggtgagaa gtatgacgtt atccagggct
     1321 gttttgaaaa gtcaaacgtt gacgctaaca aggagatcac ttctcttatg gaaacgcaga
     1381 gattatttga ggcgagcagt gctgtactga agtatatgga tacgataaac ggcagagcgg
     1441 caagcgagat catcaaactg taaaatgact gaatgtgagg atatgttatg aacatagcat
     1501 tctacacggg taagacgggg cttattgcac agcaggaagg tcttaacgtt tactcgaaca
     1561 acatcgcgaa cgtgaacacg gtgggcttca aggcatcgcg cccgagcttt gcggactgta
     1621 tctatacggt gcagagaaaa acagagcccg actggcagac aggtcacggc gagtatgtgc
     1681 acagcactca gcttatgtac agcgagggcg tatttacata taccgataac gagcttgatt
     1741 ttgcgatacc gacggaagag ggcttctttg cggtaatgga taagtacggc gatgtaaact
     1801 acacaagagc gggcgacttt cagatgtcgc agataggcga tcactgggag cttgtaagcg
     1861 ctaacggaga atttgtgctt gactatgagg gaaaccacat tacagttcct tttatagagg
     1921 gtacgagcaa gcccgactat gatgcgcttt caaaaatgat aggtgtgtac acattcgaca
     1981 acaactttgg actcgaactt ctgggcagta acaagatgac tgctaccgag aggtcgggag
     2041 aggcggttgc cgacaggagt atcgacaaga taagaatggc actcgaacgc agcaatacgg
     2101 acattgcggg cgatatggta cgcattatcg aaacacagcg ctcttatcag atgagcgcaa
     2161 gagttgtaca gacagcagat gagctggagc gtataaccaa caacctcagg tagtaaagcc
     2221 cgggggatat catcttatga tgtattttga tcgtactgcc gggagcttac ccggcagata
     2281 cggttattta aaaacaaaca gttaataatt tcagaggtct attatgataa acagttacga
     2341 cgatttaagc ccggtacagc ttgatgtgct caaagaaata ggaaatatcg gctcgggaaa
     2401 tgccgcaacg gcactgtcac agctccttaa caggtgtatt gatatgcagg tgccgcaggt
     2461 aaggcttatg gnnnnnnnnn nggacgtggc tgacgcgata gagtccctcg gttcgcccga
     2521 caagctggtg gtaggaatac tgataaggct taaaggcgat gcggacggaa tgataatgtt
     2581 cttgcttgaa gaagcatttg cgaagactat cgtcacggga cttacgggag atcgcccgtt
     2641 ttcgcttcat gagatgaatg ccgatgatat atcggtgctc agtgagatag gaaacataat
     2701 gggcggaagc tatgtgaatg ccatagcgga tcttggcggt atgacgatag atatgtcggt
     2761 gcctgcgctg acgaccgata tgctcggggc tataatgact gtgcccgcaa cggagctttc
     2821 ggaagcgtat gaaaaggtac ttatgataag cgaacaattt ctgatagatt cagttgagat
     2881 acaatccgat atgcttctca tacccactgt tgagtcactg aggacgctcc ttgggaaact
     2941 gggggtagaa aaccagtgaa caggataata gtggggatat ccgatcagaa gctgtgcaag
     3001 tcacccgatg ttctggtaac atatgcactt ggcagctgcg tcgggatatg tatgatagat
     3061 aaggcgctcg gcatagcggg gctggcgcac ataatgctgc cggacagcag tgcgataccg
     3121 agcgataaaa acaagttcaa gtttgcggat acgggaatac agctgttgta tgacagtatg
     3181 ataaagagcg gtgcggcggc atcgaggatc accgcaaaaa tagcgggcgg agcgaatatg
     3241 tttgcgacaa catcgcctgc tatgtcgata ggtgaccgta acgttgaggc aaccaaaaaa
     3301 atgcttgcaa agctcggagt accgataata gcatcggata cggggcttaa ctacggaagg
     3361 acggtatctt tcaatgcggc ggacggctcg cttgagataa tgtccagtct taagggaaac
     3421 aagacgatat aaagatatat caacacatca cacagggagg tgcactgatg gaaagaaccg
     3481 aaaggcagaa gatggctgac gcttctatgt cagagcttat agaactggaa aaggcatcaa
     3541 atacatcact tgaaggcatc gagctgaaca acggcggaac tatgcaacgc ccgcagggaa
     3601 caaatccgct gcttaatgtt gatgcatccg ctaaggtcgt tgtaagcgac aacaagcttg
     3661 aagccaatat gtgcgtattt tcacctcagt tttcgggcaa agatatcacg gtggaagcta
     3721 tgcgtcaggc gctcaaggac gagcgtgtcg tatacggaat agatgatgag ctgcttatag
     3781 agatagccga gaacaagctg tatgataaaa ttttcactgt ggcaagcggc tatgcggcga
     3841 tagacggcga gaacggcagg gtgaacaatc tttttgatac ggataagaag cttgtgccga
     3901 gaaagcttga ggacggaagt gtcgactacc gtgatctggg tcttatcgta aatgtcaaga
     3961 tgaacgacct tatatgtgag atcacgcccg aaacacaggg agaagaggga atgaacgtct
     4021 acggtcaggt gatagctccg cgcccgggca gacctccgct gatacctcag ggtgcgaata
     4081 cggtgctttc tgccgacggg acaaagcttt ttgccgctga cagcggaaac cttgtctata
     4141 agggcggaag attcaatgtt gaaaccacgt tccagatctc atcggacgta gatgtaaaga
     4201 caggcaacat agatttcctt ggcgatgttg tgatcaaggg cagcgtacag g
//
LOCUS       DAWAOG010000128         4248 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2679_length_4248_cov_3.196756, whole genome shotgun sequence.
ACCESSION   DAWAOG010000128 DAWAOG010000000
VERSION     DAWAOG010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2679_length_4248_cov_3.196756"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1265 a          896 c          891 g         1196 t
ORIGIN      
        1 atcatatccg gatcaacgat cgcaatgatc ggcatatcat gcggatccac acagaccaat
       61 tttctttttc tttccacatc tgtgatcaca tagttgatgg taacttctgc agctgttcct
      121 gcagttgtcg gaacggcaat gattggaata cagggttttg ttgtaggcgc cacaccctcc
      181 agacttctta cgtcttcaaa ttccggattt gcaatgataa taccgattgc ctttgcggta
      241 tccatagaag aaccgccgcc aattgcaaca atgtaatctg ctttggcagc tttgaatgcg
      301 gcaacaccat tctgtacatt ttcgatggtc gggtttgctt tgatgtcaga ataaatttca
      361 taagcaagcc cagctttgtc aagaacgtct gtcacctttc ctgtgactcc gaatttgatc
      421 agatccggat ccgagcagac gaatgctttt ttgaaagctc ttgcctttgc ttctgctgca
      481 atttcctgga ttgctcctgc cccatgataa gatgtttcgt tcagcataat tctatttgcc
      541 atttgtatca ctctcctttg ttttgatttt aacaattctc gggatgtaca ttgtgattac
      601 ggtaaaaagt gatgcagagc atcagacgcg agttatggag tcgatgatcg caaatgaaat
      661 cagtatggaa cggagcaggc acatccggac actcaaaaaa tacttacaga gaaatataca
      721 gagtgccata aagatgccta atgcgtattt gtagtagtca gggtcatcca tcatttcacg
      781 gacagcctca tactggggtt ttccctgaca gagcatacca atataggtga ggagcacgtc
      841 tccatttttt atctgtttct gcagataatc ttttgaaatt ggagcacggt tgattttttt
      901 aacaaaactg ctttttccaa gtatttgccc tacgaatacc agaccactgg gagtaatgag
      961 ccgctcatcg ctgaattcaa tgtgtatttt cttcattgca ctgcctccgc ctatcttttg
     1021 atactttcat tatacaggat aatgcatgat gatacagtaa aaaaacaaaa ttttgtttaa
     1081 cctagtgggt gaaataaaat gtattgcgaa aatcagtttc cagcaggtat tattgctgaa
     1141 aaaaagggat gtaacactat ggtaagtttc acggaataag gtggattttc attgacgcat
     1201 ataacacctt acaatataat aaaagcaaat atatagctta taaaggaggt tccttaatga
     1261 cgaaaaaaat aaccgcaata ttcttggcat tgtgcatggc aatttccgtt ttgccgatga
     1321 ccattcaggc ggcatcaaag cccgatatta aagttggcga ctatgttaaa atgggcgcgt
     1381 ataacaatgc ctcaattctt tggcgatgtg taagcattga caataacgga ccgcttatgc
     1441 ttgcagacaa aattgttgac acccttgcat atgatgccaa aacaaatgac aacagcaatt
     1501 caaaatcaca cagcagaagc tataagcgtg atgattacgg ttctaactat tggaaagaca
     1561 gcaatatgcg ttcttggtta aattcgaccg cagctgaagg caaagtcgat tggctttgcg
     1621 gcaaccctcc gaaagacgga tatgtaagcg gtgtgggagc gtacaacgaa aaagcgggtt
     1681 ttctcaacgc tttttcaaaa tctgaaattg cggcaatgaa aaccgttacc cagcgttctc
     1741 tcgtttctca tcccgaatac aacaagggca tagttgatgg agacgcaaat tcggatttgt
     1801 tatactacac cgatatttcg gaagcggtgg caaactacga cagttcatat tttgaaacta
     1861 caaccgaaaa ggtttttctt cttgatgtaa agcaggctaa tgccgtgtgg aaaaatctca
     1921 aaggttatta tgttgcgtat aataatgacg gtatggcttg gccttattgg cttcgcactc
     1981 ctgttaccga ttgcaatcac gatatgcgtt atatcagttc atcgggtcag gtcggtcgtt
     2041 atgctccgtg gtattccgat ttgggcgtaa gacctgcatt ttatctcgat tccgaatatt
     2101 ttgttacaac ttccggcagc ggctcacaaa gcagtcctta tatcggctct gctccaaata
     2161 agcaagagga tgattataca atttcggaac ctgccgagga cgcaaatccc gattggaatg
     2221 tcagcaccga gcaaagcatt cagctcaccc tcggcccgtg gtattcaaat gacggaaaat
     2281 attctaatcc taccatccct gtttatacca tccaaaaaac acgcagcgac acggaaaata
     2341 tggttgttgt cgtttgcggc gagggataca caaaaagtca gcagggcaaa ttcatcaatg
     2401 atgtcaaacg actttggcag gacgctatga aatatgaacc gtatcgcagt tatgccgaca
     2461 gattcaatgt ttacgctctt tgcacagctt ccgaatcgac ttttgacaat gatggaagca
     2521 ccttctttga tgttatagtc gacaaatata attcgcctgt tatttctaat aatctccacg
     2581 gaagtcagtg gaaaaatcat atttttgaga gatgtatcgg tcctgaattt atcgagaaaa
     2641 ttcacgatgc tcatatcaaa aaaaagtgtg atccaaacac aattccgtcc ggttcggaat
     2701 atgagcctta ttattatgtt cacgattata ttgctcagtt cgctatggtt gtaaacacaa
     2761 aatcagattt cggcggtgct tataataacc gtgaatacgg cttccattat ttcatttcgc
     2821 catcagacag ttatcgtgcg tcaaaaactt ttgcacacga gtttggacac ggtttgctcg
     2881 gtctcggtga tgaatacagt aacggatatt tgcttgacga taaggaactt aaatcactca
     2941 atctttcctc ggttgaagac ccggaaaaga taaaatggcg acagctttta ggttttagaa
     3001 acacatatac ctgccgcaat gcttacggct caaaaatgct tgtttcaagt tatgagtgca
     3061 taatgagaga cacaaactat caattctgtg aggtttgccg tttgcagggc ttcaagcgaa
     3121 tgtctcagct tgttaaggat gtagaccttt atgttgcaac tcccgaggtt aaggaatata
     3181 cgggcgctta ttcaaagccg tctgatttta ccgaccttga aacaagttca tattataatt
     3241 acacatataa tcgcaatgac cgacttttga gcggcaacag caaaagcaga tttaatacta
     3301 atatgaacgg taaaaaaatc gagcttagaa ctgttattca aaacatttca gataaaaatg
     3361 cacggcaatt aaaattcaaa atgtggatta agcactccga cggaagcgtt gcgaccgatt
     3421 catcggggaa tccgcttcaa acagtacaaa cctttgacat tcctgtttgg aatgataagg
     3481 caaatttctg gccgctcggt gctttggatc acatcaaaag cgactttaat tcaggcttaa
     3541 agagttgctc gcttatttat caaattccgt ctgatgcaca gcttaagagc ggcgataccg
     3601 tggcatttca ggtgctcgat gaaaacggaa atgtgcttgc agacgacaat acggaaacac
     3661 agcgctacac aaccgtttca attcagtata aatttgagga cggctctgaa attccaaaca
     3721 ctgccggcgg aacatttacc gtgccatacg gcacaaagct tgatttaaca ccggcaaaaa
     3781 cactttatga ttatgaattt ataaaggttg acggcttgaa taagcctatt gtttcggacg
     3841 gaacggttgt tacatattat tacaaaaata aaaacgaaga acacactcac aatctgactt
     3901 tggttgctgc gaaagctgcc acctgcacta ctgcgggcaa ttccgcatac tacacctgcg
     3961 atggctgtga caagtggttc gcagatgcaa caggatcggt agaaattacc gataaaacca
     4021 gtgtgaagat tccggctacc ggacataccg caggcacaga atggaagtct gatgatacta
     4081 accactggca tgaatgttct cgttgccacg acaaaaagga cgaagcggct cacgattacg
     4141 gttctgacaa tgtttgcgac acctgcggat atgacaaaac tgtaccgcat acgcacaatc
     4201 tgactttggt tgctgcgaaa gctgccacct gcactactgc gggcaatt
//
LOCUS       DAWAOG010000129         4246 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2681_length_4246_cov_2.687425, whole genome shotgun sequence.
ACCESSION   DAWAOG010000129 DAWAOG010000000
VERSION     DAWAOG010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2681_length_4246_cov_2.687425"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    493..592
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3632..3641
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1011 a          986 c          841 g         1298 t
ORIGIN      
        1 gcattccgca ctgttaagcg gtacgataaa cacgaccgat ttagtccgat ttatattata
       61 caccctattg tctacaaaat caataggttt tataaaaaat tttccgaaaa atatcggcag
      121 tgggaaaact cccactgccg acaattcggt tattcacatt cttctgcaat aaaccgtaag
      181 gtttattcga tagcaattac gttatccgtt tcaacctgcg gagcggcttt cggaacagat
      241 acctttaaaa taccgtcctc aaactttgcg ccaacgtcct tagcctgtac gccctcgcct
      301 acatagaagc tcctgctgca ctgaccttca taacgctcac gtctgatata cttaccgtcc
      361 ttcttctcct tttcgtcctt ttcaagaccc ttggaggcgc tgatcgtaag ataaccgccg
      421 tcaagtttca gcgatatatc ttctttcttg aagcccggca gatcgatatc aagctcgtag
      481 ctgttgtcgg ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcgaagaa
      601 attgtcaaat aagttttcat tgaaaattgc gggtaacata agtcatttct ccttccatat
      661 atcggacggt cggtttatta tccgaccgtg taactgctat gtgtgtgcct gctgtcgttt
      721 attcgatagc gataacatta tccgtttcaa cctgaggtgc ggctttagga acagatacct
      781 tcaggatacc gtcctcaaac ttggcgttta cgtccttagc ctgtacgccc tcgcctacat
      841 agaagcttct gctgcactgg ccctcataac gttcacgcct gatgtacttg ccgtccttct
      901 tctccttttc gtccttttca agacctttgg aagcagtgat cgtaaggtag ccgctgtcaa
      961 gcttcagcga tacatcttct ttcttgaagc ccggcagatc gatatcaagc tcgtagctgt
     1021 tgtcggtttc gcggacatct gtcttcataa cgttctttgc gtgtttgccg taaagcggat
     1081 cgttcttgcc gaataccgac ggcatataaa ggtcatcatc gaagaaattg tcaaataagt
     1141 tttcattgaa aattgcgggt aacataagtc attcctccat tcattccaaa gcggtctgtt
     1201 ctttaaacgc agtgccgaac atttgttgcc cttgtacctt ccggagaccc tctctctttc
     1261 tttgtacaac tacattatag cacacttttt agcactgtca ataggggagt gctaattttc
     1321 acaaaatctt cttaaaagtg tgacaaattt gacattcagg aacgaaaaca cgaaatgtaa
     1381 ttgcgcatta tcggcgcttt tgcgtgcgaa aataacgtcc cgctgtaaga aaatgttcct
     1441 acagcgggac gcttaaatgt caatattatt gtatcatgcg tacaggctca tgatagtcgg
     1501 ggtcgtttat catagctgaa tttatcgttg tgttattaag agcagtcttg ccgttcgtaa
     1561 agcctacatc taccgaataa gtgcctgaga gagaagtgat ctgtatatca cttcctgcgg
     1621 cgcacagagc tccttttccc tcagagctgc ctataccggc aacctgttcg ccttcgatat
     1681 gaattttaaa gtcggagcta tttatattta tgttgcattc ggtcttgcga cagccgacag
     1741 ccgcaccgcg cattgccttc ataagaagat cgaatgaagc attacggata tctatacttg
     1801 atggattatc accgtcaagc gcagagagcg cgcccacgca acctgcacgt atgccgagcg
     1861 ctctcagtat gagctttgtg ccgtttattc ttatatccac atatccgcac agtgagccga
     1921 gagctaccgc gttgtcgccg gaacagttta tatctataat ggcgttgcct gtttcgatgc
     1981 cgcagcttcc gtcatatgag cccacgccta tgcagtcttt tccggtcgat gacatattta
     2041 tgctgccgcc cagcagtttt atcggcattt cgcttgatga cacacctccg ccgatacaga
     2101 taccgtgatc tccgtttgcc tgcatctcaa tactgccggt catatctatc gttatttctc
     2161 cgaaagtatc gttataactg cttcctatac aacagccgtc atttctgtat gagtcaatat
     2221 aaagacttcc gtcaccggtt attgtaagct ttgctgtgtc gggtacaagt ataccgtcgt
     2281 agttaagtgt gttctgacct ttgatctcta ttgcagcatc ggaattttcg cctatctgta
     2341 tgcactgacg cataccgctc ttgatattca cattatcaag tatgatcctg cattttgaac
     2401 cgttttcggt ctttatcaca aatgtggcat tatcacagcc gttagccgta agatgaaccg
     2461 aagcgctttc gactataact cctgtgtaac gctcggaaag cattttttca atatctaatt
     2521 tttcgttacg gccgtccttt acggtataat atcgagctac ctttatattg ggacggtgca
     2581 tattgagcat tctcatctgc tcggcaatag tatccgatat atcggggacg atttctttct
     2641 gcggtcttgc cgtgtagaag ccctgtatat agtctgcacc tctgcgtata gtaacgctca
     2701 tctcgtcata tgtttcaacg ccttcggcaa ggaccttaat gttattcagc cttgcaaaat
     2761 cgataattcc tgtcaggaag tgctgttttt tgacgtttgt attgatatct gtaataagcg
     2821 agcggtcgat cttcaataca tcgggctgca gactgagaac cgccgcagtg tttgaataac
     2881 cgcttccgta atcatcgatc gcgatcatac agccgtgtga tttgaacagc tgccgcagtt
     2941 ttgctatctt ttcatctgta agatccgcct gctctgtaaa ttcaattacc agctgaggca
     3001 ttatatcgcc gtacttttcg cacagtctgt taaaatcata ctctgttatc atatggtccg
     3061 gcacagaatt tatgaatatc tttttttctc cgaaacgcga acggttcttg ctgactatct
     3121 caaggacatt ctcaaaagta agccgctcta tgtcatacag ctttccgtgc tcgcgtgcaa
     3181 tgcggagtat atcgagcggc gacgaaccct taggacgcat aagcgcctca taggcataaa
     3241 cagaaccgtc aacggtcgat actatcggct ggaagcagta actgaaattg ttgtcgtcaa
     3301 taaggctcgt cagaagcttt gaatcggaat agctgttgca ctccgtaaca tattcttctg
     3361 ccgaaaactc cctgtttatg cttccgagcg ctttggctct gaaaagtgca aagtcggcat
     3421 agtttatcat catatcgata gtagccgcct gcgtaggata ccagcagtag cctacagtta
     3481 tagtgaatgc ttcactgcgg gtttctatgg tatttccaaa ttcatcggtc agtctgcacc
     3541 tgctgactgc atcgtacatt ttctttataa tatcgttaat ttcttttttt gccatctggc
     3601 ggaagaaaac taaaaagtct ttgtagcttt tnnnnnnnnn nctatgatga cattttcgga
     3661 attaaaacgt ttaagtgcgt ttgccgccgt ggcaatgcaa agtgatgtct tatcataatt
     3721 aagctcgctg tctacacgtt ctataccgtt gatatgtaca gccgccatac agcattgagc
     3781 ggtgccgctt atctgactta tcatactgcg tacacggcgt atgaagtagt cgcgtgtgta
     3841 catacccgta agcgtgtcat actctttgat atcgtccttc gggttgcgcg cctgcattat
     3901 ctgcgttacg tcctgtataa agccgagcag atagtctgta tcatatacta tctttatctt
     3961 tatgtagctt gtttctccgc cggcagtata catatatata ttcttgtgac cctctacctg
     4021 attttcgttc agactgtcaa gcagagcgta aaacttttct ttattcatat tgtcgccgct
     4081 gatatgaaga attgcggcaa cgtttttgtc atagtatgct tcgttcaggt cgtgcggaag
     4141 tttaaataca cctatagtgg ggaagttatc cagaaatata ttccaatcat ggcggctgtt
     4201 gccgtcttta tcaaaaaagc tcatatgata tcctcccaaa ctgtaa
//
LOCUS       DAWAOG010000130         4211 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2701_length_4211_cov_1.865977, whole genome shotgun sequence.
ACCESSION   DAWAOG010000130 DAWAOG010000000
VERSION     DAWAOG010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2701_length_4211_cov_1.865977"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          961 a         1105 c          862 g         1283 t
ORIGIN      
        1 ccaccagccc tgctcctgca caaatgcaag ataagcgaca tcttccttga aagagaagaa
       61 cgccttatgc agaagctcgt tcttctgcgc tatcaccttt tcatagtcga ccgcagacgg
      121 atcttcgccg tactctctgc cttttatatc atcgggtata agaagtccga gctcgaccag
      181 ctcccacagg tcgataagca gagggtttcc tgcgaatgcc gacggcgact gatacggagt
      241 atccgcatag cctgtcgggt gaataggcag tacctgccag tacttctgcc ctgccgcttt
      301 aagccagttg acaaattcaa acgccggttt tccgagcgtt cctatgccct cgtttgcagg
      361 aaggcttgtt atatggagta atactccgct gctgcgttta agttccataa tatcgccttt
      421 cggaattaaa tcttactttt cgtcattcat acttgttctg aacggagcaa caggtattcc
      481 gttcttgccg tacagcgtaa catcgccgta atttgtccaa agataacgta cataagcggg
      541 ctttttgacc tcgtctgcat aaacgcttat tgttccgtcc tcgtctatta cggcgcacgc
      601 aggcttatag tccttatcag ctcctgcaac ttcaaagcct gttatgccgt tgtcgccctt
      661 tatgtcaaag ccgctctcgg cgtggtcaaa gcttattacc gctctgtcgc ccgatactgt
      721 catattctta tatacaggac cgaatgcttc ggtttcgttg tcgccgtaaa catggtgcat
      781 agcctgtaag tacagtctgt gacctaccgg tactttgttc ttggggtgta tctcgttgaa
      841 ctcgccgcag tcaaggataa ctgcgatacc tgtattcttg accgtcttga aggtacgcat
      901 ctgcgcctct cttataaggc accagtgctt ccagtcaggg tcggctttat agcggtgcat
      961 cgggagctgt acaaacataa acggaagctc atcgtctgcc caatcatctc tccaaagctg
     1021 aataagcgcc ttgaacagct tgaaatatgt cttaggcctg ttgtcatcgg tttcaccctg
     1081 atagtagata acacccttga tagtatacgg gcatacacgc tttaccatac tgtcataaag
     1141 cgctgtagcg tggaaaggat ttaaaggtgt cataggaccc ggatacttgc tgacaccgca
     1201 gtaagcctga cactcgtccc atgttccgtc gggatgtgcg ctgtagaact ctaccgactt
     1261 cttctgccac tcctcctcat actttacata gtcgagatac tccttgtcca tttcttctct
     1321 tgaacgacct gctaccgcat tatcaaagtc atcaagataa gccttaaggt cggtatcttt
     1381 ttcaagtgac tcacggctca tccaatagct tgcgcttgtg ccgccccagt tacagccgat
     1441 tattccgaca gtaacgccca gtctgtccgc aagctcctta gcatagaaat acgcaactgc
     1501 gctccacgct cttgcacttt cacagtcaaa gcagctccag cctgtgtttt cttcgtcacg
     1561 gaagaacttt tcgtccatat tgcagttctt ctgtgtgtag tagtaacgta cattctccgt
     1621 cttgtcgttt gcaagatgct ccttgccgcc catgcagttc tgaagctcaa gctccatatt
     1681 gatctgaccg cccgcgagcc atacctcgcc tatcataacg ttaccaaaca cgatagtttc
     1741 gtcaccgttg cttatcttca tctcgtaagg accgcccgct ttcattgcag gaagcgtaac
     1801 gcaccacttc tcatcggctg cctgcgttac ggcagtgttt ccgcatatag aaactattat
     1861 gctttcgccg tccttacctg tgccgaatac tcttacattc ttatctctct ggagaaccat
     1921 attactgctg aataccgcag ccgctttaaa tttagccatt gtttttcctt tcacttgaca
     1981 ttttcgtttt tcttcttgtg ttttgctttg gcaagaacct tctgtatgtc ttgtcagacc
     2041 tgtccggttt tactgttctg tcagttgcaa aagcaggtgc gggcagatct ttatttataa
     2101 gagaatcata caaattctca tctttttcat cggacagtac ataccgtttt gtccaaaggt
     2161 attccctgtc tgctttcttt tcaaacctta ccgttacctt tatcgagtgc ttattacagt
     2221 gcatgaccgc catatagttc atcggtctta gccacacaag cttgcctttc agcgtagcgg
     2281 gctttccgca tatcgggcag acgtatcggt taagcggcga caatctcggc acactgttca
     2341 tacgggcggt ctttactgtt ccgtacgatg tctgacatat tctcctgaaa ggatttgtat
     2401 gtgcatagtc gacctttctc tgccactcta tttcactcgg tatattattt attttagtaa
     2461 ggacaagtgc tgtgtagtat gcatcattta ttgcgctgtg gtaatcaagc tcctgctgca
     2521 cgccggtgat ctcgaccgcc gactgaagtg ttatctgcgg acgatcaata tcatactgcc
     2581 ttgtcatgag cggctgaaga ttgaaccagt cggaaagcca gcttacatca tagttgtaaa
     2641 acaaacactg tccgcgcaat gtcggtatat catcgggtcc ccatgttgct ataatgctgt
     2701 ccccgaagcc ctcgcaccac tgtctgaaac gctccatgac gatcaccaga tcatctgcat
     2761 ccttaagatc gtcctttgtt atgcctgtca gcttctttat tctgccttta agctccgtat
     2821 aatatctcgg ctttacaaat tccgaaaaga aattttcact tatctttccg tcggtaaccg
     2881 ccacagcgcc tatctgtatt atctctacct gcaacacctt agtgcccgct atccgcgttt
     2941 tttccttgca tacaggttga ctccattcca tatcaagcac aatgtatgtc acccgtgcgc
     3001 ctcccttccg tatatcatat aatataacac tcaaagcccc gcatagtcct gcggggcata
     3061 agtatttata tcgcgacttc tattttctcg ttgaaatcgt gatatttata gtctttcagc
     3121 gtaaagctgt ttttagtgaa tttatagaaa tctgttatat cctcgaccac catttcaggt
     3181 gcgggatacg gctctttctc aataatacgc tttaccgcat caatgtgtct gtcatagata
     3241 tgagcgtccg ctataacgtg tacaagctcg cctgccctga gtccggatgc ttttgcaaac
     3301 attatgagaa gcgcggcata ctggcacaca ttccagttgt tcgcggtgag catatcctgc
     3361 gaacgctgat tgagtatagc gttcagcgta tcgccggtga cattgaacgt catactgtat
     3421 gcgcaagggg caagtcccat ctcatgcaga tccttgtgat tgtacatatt cgtcattata
     3481 cggcggcttg cggggttatg cttaaggtca tacagcactc tgtcgacctg gtcaaactca
     3541 ccctcggggt aaatactctt ctgtccgagc tgccagccgt aagccttgcc tatcgtgccg
     3601 ttctcatcag cccacgaatc ccatatatga gtagataaat cagcgaccct gttgctcttc
     3661 ttctgatata tccagagtat ctcctctatc gcagagcggt aaaagatacg acggagcgtc
     3721 attaccggaa actcttttct gaggtcatag cggttcacca cgcagaactt ttttacggta
     3781 tgcgcgggtg ttccgtcctc ccagtgaggt cttacctcgc tgtctgtatc ccatgagccg
     3841 ttttctatta tatcacggca gttttcaata aaatatttat cggctaaact cattatgcgt
     3901 tatgcgtcct tatatctttg ataacagctt tgttgtcgag aaatctatct ggtcgaatgt
     3961 acggctctcg aacatattaa gactgtcgat accgctcata ttatagatag aaacaccggc
     4021 gcttgaagcg tatgtcttta cgttcttgag cagtgtctgt gcttcctcag ggtagtcgta
     4081 ctttttctca tcgtttacaa gcgcctcttc atagcctgtt atctgcgcat cgtaaattcc
     4141 gtcaaagccg tatatgtata tctccgaaaa tccttcatac agtgcaagct gtaaggctat
     4201 ataaaggtcg t
//
LOCUS       DAWAOG010000131         4209 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2707_length_4209_cov_2.398652, whole genome shotgun sequence.
ACCESSION   DAWAOG010000131 DAWAOG010000000
VERSION     DAWAOG010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2707_length_4209_cov_2.398652"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    326..335
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1212 a          852 c         1098 g         1037 t
ORIGIN      
        1 catctgatga tgcttgacga tgacgattac aacgagtcga tcgaaaacat aatagatact
       61 cagtcggtaa atgccgaata tgcggtagcg cttacatctg ataatttcgc agagatgttc
      121 tcatcgatgg acgatgcata tatgcaggca agagccgcag acgtaaggga tatttcaaac
      181 cgaattatcg caaacctgac aggtgcaaat accgacagtg ccgcgaccga tgaaaagtcg
      241 gtgatatgtg ctgacgatct tgcaccgagt gaaacggttt cgcttgataa ggacaaggtg
      301 cttgcatttg ttactgctca cggctnnnnn nnnnngcgat acttgcacga aatatgaata
      361 tccctgctgt gataggtgtc ggcgacagct ttttaagcga gatacatgag ggagaaacag
      421 tcatagtaga tggttttacg ggcgagataa tcgttgctcc cgatgagctg acattaaaaa
      481 cctataccga aaagcaaaag cgtgacgagg agcagaaaaa gctcctgcag gagcttaagg
      541 gcaaggaaaa cgtaacaatt gacggtacta agataaacgt atttgccaat atcagcggca
      601 ttgacaatat aggtgcggtt ctcctcaatg atgccggcgg cataggtctt ttcagaagtg
      661 aatttttata tcttgaaaat tccgacttcc cgacagagga gcagcagttc cacgcttata
      721 agcgtgtgct tgaaagcatg gcgggaaaga aagtcataat cagaacgctt gatatcggcg
      781 cagataagca ggtggactat ttcggactta aaaaggaaga taaccccgcg ctgggactgc
      841 gtgcgatacg catctgcctt acacgtcccg agatattcag aacacagctc agagcattgt
      901 tcagagcgtc tgtttacggc aacctcggta taatgttccc gatgataaca tcggtcagcg
      961 aggtcgagcg tatcaaggct atctgtgagg acgtaaaagc cgagcttaag tccgagggta
     1021 tcgagtattc cgacaagaca gagatcggta ttatgataga aactcccgcg gcggcaatta
     1081 taagcgaccg cttagcaccg atggttgact ttttcagtgt cggaacaaac gaccttacac
     1141 agtatacgct tgcgtgtgac agacaaaacc ccgatgcgga ggaatttgtc gatactcacc
     1201 acgaggctat tctccgcctt atcgaaatga gtgcgcagaa tgctcataag aacggagcgt
     1261 ggataggaat atgcggcgag cttgcagcag ataccactct tacagaaact ttcctgcgta
     1321 tgggcattga cgagctttcg gtttctccga cattcgtcct taaggtccgc gatgcggtga
     1381 gaaagacaga tttgtcgaaa taattatccg ctgacgactg catttccata aaaaattaca
     1441 gcagctgcgc tttccccttg cgtttactgc tgtgattttt ttggatattg cgcaaacaga
     1501 cggtcgttgc atctgtttaa aaaatgtccg acttatttac aaccgcgctg tcatcggcta
     1561 tctgtaattc agtgtaattt tgcacaattt tctcccgtaa accgtcgaaa attatcgttg
     1621 aaaacagtaa cggttagcaa gttacaactt attgacaaat aaaaagtatg gtgttatact
     1681 aaataaaaac aaaaagtaaa atctgtgtaa gaagatctga aggcagatca aagcgcctac
     1741 ggaatagcat tgcagaatat ccgctgtcat tccgttttta ttatgtaaag atagttagcc
     1801 aaggcttact tatttgcgct ttcgcttttc tgcccgggca aagtacacag acggctctga
     1861 aaagagtaat aatacaagga ggtcaaatta tgaactgggt aaaattaggt atatttgcag
     1921 gcggtgtagc attcggctct gtaggactga agcttctcgg cagtaaagat gctaagaaag
     1981 tatatgcgca ggtcactgcg gcaactcttc gcgccaagga atcggttatg aagaccgtta
     2041 caaatgttaa ggaaaatgca ggtgatattc tcgccgatgc caaggatatc aacgaaaagc
     2101 gtgacgctaa ggaagctgtt atcgaggacg aatgtgactg cggttgtgaa accgtaacgg
     2161 aagaagcata agactgacag aaaaccgacg gtacgctttg ttactgtcgg tttttaattt
     2221 cggtttttat atggagatga caggaaatga aatgtaaaat actacacgag tctaaaggca
     2281 gactgcgtgt gcatatgttc tgcggcaaga tgtcgctcga caatgccgat gtgcttgaat
     2341 attatctgcg ttcggtaaac ggcgtgaccg atgcagcggt atacgatcgt acttgtgacg
     2401 cggtcatagt attcaccgac agaaaggcag tgcttaaggc gctcgccgct tttgcttttg
     2461 aaaaggctcg gaagatggga ttagtgcccg accatacatc aagaaagctc aaccgtgagt
     2521 ttgaggataa gcttgttatg acggtagtgg cgcggtttat atcaaagacg tttatcccgg
     2581 cacctataag ggcgttcatc gctgttataa agtcgtggaa atatataaag cacggactga
     2641 aagcgcttct gagcggcaag ctgtccgtag ctgtacttga tgcgacagcg gtaacggtat
     2701 cgcttctgcg ccgtgacttc aagacagcgg cgtctgtaat gtttatgctg aatatcggcg
     2761 agatacttga ggactggacg cataagaagt cggtagcgga tcttgcgggt gcaatgtcgc
     2821 ttaacgttga aagcgtatgg gtaaagacag gcgacaccga aacgctgata tcgataaacg
     2881 atgtacatga gggagatcag gtagtgatcc gcacaggcaa tatgataccg cttgacggca
     2941 aggttgtatg cggtgaagct accgtaaatc aggcatctat cacaggtgaa tcgctccctg
     3001 taagaaagag cgagggcagt tatgtctatg caggtactgt tgtcgaagag ggcgaatgtg
     3061 ttatctgtgt tgataaggca acaggcggag gcaggtatga ccgcatagta aaaatgatag
     3121 aggagtcgga aaagctgaaa tcttcttctg aggataaagc atcaaggctt gcggacaggc
     3181 ttgtgcctta tacgctcggc ggaacgatac ttacttatct tctgacccgt aacgtaacca
     3241 agatgttagc cgttctgatg gtcgactttt cgtgtgcgct gaagctctcg atgcctatcg
     3301 ccgtactttc ggcaatgcgt gagtgcagta attataagat aagcgtaaag ggcggtaagt
     3361 tccttgaagc agtgtccgaa gcggatactg tcgtatttga caaaacaggt acgctgactt
     3421 atgccgcacc gactgttgaa aaggtaattc ctttcggcgg caaaaacgcg gacgaaatgt
     3481 tacgtcttgc ggcttgtctt gaagagcatt atccccactc aatggcaaat gccgtggtag
     3541 ccgccgcaaa ggaaaaggga ctgagtcacg aagaatatca ttcaagcgtt gaatatgtcg
     3601 tagcacacgg catatccagt atggttgaga ataagaaggt cataatcggc agtgcccact
     3661 ttgtatttga agatgaggga tgtgtcgtac ctgagggtga cgaggagctt ttcaaatcgc
     3721 tttcgggtga atattcactt ctgtatcttg ctatatcggg tgtgcttgcc gcagtgatct
     3781 gcatatcaga cccgttgaga gctgaagcgg cagatgctgt taaagcgctc cgtgagtgcg
     3841 gtataagcaa gatagtaatg atgaccggcg acaacgagca gacagcgaag gcagtagccg
     3901 ccaaggtcgg agttgatgag ttccacgcag gtgttctccc cgaggacaag gcgaacttca
     3961 taaagagcga acacgaaaag ggcagaaagg tcataatgat aggcgacgga gtaaacgatt
     4021 ctcccgcact gtccgaagct gatgcgggta tcgctataaa taccggagcg gcaattgcaa
     4081 aggaaatagc ggatatcacg gtatcgtcag aggatcttta tatgctggta actctaagaa
     4141 agataagctc ggctttgatg aagagaataa acagcaacta ccgctttatt gtatcgttca
     4201 accttatgc
//
LOCUS       DAWAOG010000132         4190 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2723_length_4190_cov_2.228295, whole genome shotgun sequence.
ACCESSION   DAWAOG010000132 DAWAOG010000000
VERSION     DAWAOG010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2723_length_4190_cov_2.228295"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1557..1566
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1962..1971
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1142 a          860 c         1070 g         1098 t
ORIGIN      
        1 attaccgagt gcgacaagga agatgtcggc acaaagataa ctcttaagat aaaggagaac
       61 accgaagaag aggagtatga cgagtatctt caggactaca agctccgtat gatagtaaag
      121 aagtattccg actatatccg ttatcctatc agaatggaag tcagccatca gcaccttaaa
      181 gagggtactg agaatgagta tgaaactcat accgagatag aaactctcaa cagcatgata
      241 cctatctgga acaagaacaa gtcagagctt aaggacgagg attacaatca gttctacaag
      301 gaaaagttct tcgactatga cgatcctatc gctcatatcc acacaaagac agagggtacg
      361 gcaacatata acgctttgat gtatatcccc tcaaaagctc ctttcgatta ctattcaaag
      421 gactatgaaa agggcttaca gctttattct aacggcgttc tgataatgga taagtgcgca
      481 gatctgctcc ctgattattt cagctttgta aagggtcttg tagactctgc cgacctgtcg
      541 ctaaatatct cgcgtgagat gctgcagcat gacagacagc tcaagataat cgaaaagagc
      601 cttgagcgtt ctatcaagaa cgagcttaca aagatgctcc gcaacgaccg tgagagatat
      661 gagaagttct ggaaggtgtt cggacttcag ataaagttcg gcgtatataa cggcttcggc
      721 gcaggcaagg aactgcttaa ggatctgctg ctgttctatt cttcaaacga aaagaagctt
      781 gttacgcttg aagagtacgt ttcaagaatg aaggaagatc agaagtacat ctactttgcc
      841 agcggcgaaa cggtcgacaa gatagacaag ctccctcaga cagagcttgt aaaggataag
      901 ggctatgaga tactttacct tatagacagc gttgacgagt ttgctttaca gatgatggta
      961 gactacaacg aaaagcagtt caagtccgta tccgcaggcg accttgacct tgaaactccc
     1021 gaagaaaagg aagaaatgaa ggctaaggct gaggaaagca aggatatgtt tgctttcatg
     1081 aaggaatcac tcggcggcaa ggtaaaggac gtaaggctgt caaagatact gaagtcacac
     1141 gccgtatgcc tgacaagtga cggactgctt tctatcgaga tggagaaggt gctcagtcag
     1201 atgcccgcag agcatgacgc aaaggctgaa aaggtactgg agataaacgc cgagcatcct
     1261 atctatgaaa cactgaaaaa gctgtatgaa accgataagg acaagctgaa aacatacagc
     1321 gagatcctgt acgatcaggc acttcttgtt gagggtatgc ctatagaaga ccctgttgag
     1381 tacagcaaca agatatgctc gcttatggta tgataattat atcaaaaata tcgtaagcta
     1441 aaaacggtaa caaaaacgct ccgacaggtg ttcgcctgtc ggagcgtttt cagcttatgt
     1501 caatagaatt tcggaagcga tccgagagtt aatgaatatt tgcttgcgta cattttnnnn
     1561 nnnnnngcac cacagtgcgt ttatgctcac ggcggaatct atatcaaaga tacagccgtt
     1621 ttcggtgaga gccgttatct ttgttttact gccgtagttt atcgcctgtt tgaaacggct
     1681 tacctgagga tcgtaaacgt gatttccggg atagatgtcc tcgcctacaa tatcaacata
     1741 ttcgtcaccc ggataccagt cggcactctg accgttatag acccagataa gattgttaca
     1801 gccgtatgta tttgtcagct ctttataaag gtatttccac agctttttgt aagcgtcggg
     1861 accttgtgcg ccccaccaga accaaccgcc ggaagcttcg tgcaggggtc gccagataac
     1921 gggaactccc gctttttcga gctttttgag ctgttcggca annnnnnnnn ngtcaaggag
     1981 ctttttgccg tttgcgtcct gaccgttcat tacttttgct atatcgaatt tgctgttctg
     2041 cgtatagaat ccgccccacc agccgtcaga actgttggcg gtgctgttca ggtattctgt
     2101 aggagcattc cagtgccagc agaatgtgac gattccgcct tttttatgga actcaatagc
     2161 tttgtcaacg gctgatgaag atgtgccgag tgccgttctt gacggagtgt agtccatcat
     2221 atcaagtccg agcagggcag ggtagtcgcc ggtaagattt tttatcgctt tgaattcgtt
     2281 gccgtttatt ccgccgtcac actgctgacc tgtgattaca tactttccgt agctgtcctt
     2341 aaggaaactg tacagcttct tcgtgtttgc ggtggagttc ttgttgacaa gagatgatgt
     2401 gacgttatag gtgctgttgc ttatttttgc acccgtcttt actgttattt tatcaagctc
     2461 tatccagccc catgacttgg ttacacgtag tctgtgactg cctgcggtga ggcttacggc
     2521 gctgacggcg taatcggtga gcttgtcggc ggtgctgctg aatgaaccga cctttttacc
     2581 gtcaacgtac aggtcgttct cctttggaga gccgatgccc attgagcgga taactatatc
     2641 atatatgccg tctgacggga catcaatgct tatctggcag taggagcggt cattctcaaa
     2701 cttgccgaca gtttttccgc cagaagcgcc tgcgcttgtg atgacggata tatcgtttga
     2761 aaaagagctg tcttctgctt cataggtctt ggaaaaagac tgcggcgagt tgtcacttgt
     2821 ctgttgattt ttggaagaag tggtatgaga agtggtatgt accgtggttg atacgggctt
     2881 cgttgcggaa gttgaaggtt ttgctgttga agttgaaggt tttgttgcat tggtcgcagt
     2941 tgttgaggaa gaatttgtcg aggtgtttat acttgacgat gaagtggttg tgccggggga
     3001 gttttccgag gcggcggtag atgtaacggc ggagatatcc gatgtgcttt cggcacggtc
     3061 ggttttatcc gcagaatagg aagagctgtt atcgctttcg gacgaagagc tgatatcctc
     3121 cttgccggag gaagcgttgc tgttttcctt gtcggaggaa gagtcgcttt cgactgttga
     3181 ggcttcggat atagccgatg aagaggaaga ggtatccgat gacggttgag agccgttttc
     3241 cgcactgcaa ccgcacgctg aggtgataag tgccgcacat agggcgagca agatcatacg
     3301 ttttttcatg tttctttcct tttgcctgcg ttataatgta acattgcagg actttgtatt
     3361 cgattacaca gcgattatac tacaaatgtg agaaaaagtc acgcccattt ttttcttgtt
     3421 gaatattccg tgattataac caatagtgac agcggcaatt tatcacatat tcacaaaata
     3481 gcagaacaag tcttgcaaaa ttaacttaaa tggtgcataa tacttgtgta aaggtttaca
     3541 tgaaaattaa gcaagcttgc tttaataaga aaggaacaag aaatgaagta cagaaaaata
     3601 acagcggtca tacttgcagt cgcaacggca atatgtgccg caggctgtaa cggaaacgat
     3661 aattcgggaa catcgtcaag cggtgacgtg acctcatcgg gcagtggtga cagcagtatg
     3721 acaagcgata atacaacatc gggcggcaat tcgggaacgc cgcaggcgca gtttacattc
     3781 agccaggtcg ctatgggcgg cggcggattt gtaagcggcg tatttgcaac gagcgaggag
     3841 ggcttatatt atgcccgtac agatgtaggc ggtgcatatc gctacaacaa ggatgctcag
     3901 aagtgggagg caatgtcgta tgatatcagc gaggaagaca gaggtctgct cggtatagac
     3961 ggtctggcat tcggagaaaa agatcctaac agggtttata tgcttgcggg aacggaatat
     4021 ttcagcaacg gacttacctg tctgttatac tccgatgatt acggcaagac atggaaccgt
     4081 accgacctta cagaaatgat aaaggctcac ggcaacggca tgggcagagg taacggcgag
     4141 cgtatcgcgg tcgaccccaa gaacagtgac attgtttatg tcggcggcag
//
LOCUS       DAWAOG010000133         4159 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2745_length_4159_cov_2.257554, whole genome shotgun sequence.
ACCESSION   DAWAOG010000133 DAWAOG010000000
VERSION     DAWAOG010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2745_length_4159_cov_2.257554"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1041 a          944 c          925 g         1249 t
ORIGIN      
        1 gttcattacc gtgcattaca gaataacgtg ctatatcttc atcggtatcg gcacacaata
       61 tgataccaat tgtcggatta tctccctcgc tgcgcttcat ttcatcgtac atacgtatat
      121 acatatccat ttgtccgaca tcaccatgtg taattttctc ggttttcaaa tcaatcagca
      181 caaaacattt aagtatataa ttgtaaaaaa caaggtctat gaaataatct tgtttttctg
      241 tatgaatatg ctgttgtctg gcaacaaacg catatccctt tcctaattcc attataaatt
      301 tctgcaagtt tgacagtata tttttctcta aatcactttc ggtaaaatct gtatttgatt
      361 caagcccaag aaattcggta ataatcggat ttttgataaa ttcaagtttg tcaattttat
      421 aagtttgtgt tttatcgagc atttcttttt gcacaggctc tatattttgt gcctttaata
      481 ttctatagta atattgagaa gatatatttc gttgaagggt gcgtacgctc caagtttgtt
      541 gcatagcttc attctgatac catgaacgtg caacatcatc tttcacctgc agaagaattg
      601 cgtaatgtga ccatgaaagc aaaatatcag attttcctga cggtgtctgg aaaatctgcg
      661 gataagtttt ataaaacgaa tagaaagtgt acaaattaga ttttgtatat ccttttccat
      721 attttgacgt tagctcttta gacagctttg agataacatt agctccgtat tcggcacgac
      781 cggcaccttt aaggctttct tcggcgattc tgtgtccgat aagccagttc cttttaatta
      841 aagcgatatt aaccgaattg taagcgcttt tttgcgccgc ttcaataatg ccgcacatat
      901 cgctgaatat attatcagtt tgcttataat tatccataat atcattttta acagaatcag
      961 taataatatt cttcaaatta taattgcccc ctattacaaa gaattatttt acttcctccc
     1021 attcagcccg aacttccttg tatacgcatc aaagtcgaca tagcagtgat ccatatcaac
     1081 atcgccgtca atgccgctga tactgccctt ccacgaatac tgccacagga aataggtgcc
     1141 gctgtatgcg ggcttgtcgg tgtcaacgtg agctacccat acgggatatt ttgaaagtac
     1201 cgacatatca agacagctgt ttaagaacga tgcataggag tataccaccg gtatataccc
     1261 cgcattcgct atttcctcgc agaatgcctc tgtcattgag gtcagcgtct gttttgagta
     1321 gccgtttttt atgtaccact ggtcttctat gtcaaataca acgggatagc ttacatcata
     1381 gccgcgtata aggctgagta caaacttcgc ttcgtccctt gcctcctgca ccgtttcggc
     1441 atagcagtaa tgatatactc cgacaggtat attattctgc atagcacctt tcatattacg
     1501 atggaaatat gtatcgctcg tcataggata ttcatcgctt atcctgccgc ggcttgaacg
     1561 gatcatagca aagtctattc ccgacttctt cacctttgcc cagtctaccg tgtcgccctg
     1621 cgcccacgaa acgtcaattc ctgtcagtgc gtctgtcgtg taaagcgaga tcgtttctac
     1681 cgacattgcc gaggtgtctg tcgctctgcg gttatccgta cagcttccgt ttccgctgtt
     1741 ggtgaacata ccgccgagct tatatgaaca atccgctccg agcgtcaggc ttcccttgtt
     1801 taccgtttca cagccgctca ttatttcaaa tttaccgtct atgtatgaag tgccgccgaa
     1861 tgtcgtcttg cccgacaggc tgatctcgcc gtatgacgtg aatgacgaat ttatgctgac
     1921 acttccggtg aaagcggtct gtgctgtttc ggaaaggaga accgttcccg atgttgtcac
     1981 cgtcgatttt cctcccgaag agaatgtgcc gtcaacactc agctgtgagc cgctttcggt
     2041 tttcagcgcg cccattgtta taagtgaagc accattgtct ataacgaggt ttcccaatac
     2101 gtcaagggtc ttaccgtatg gcacggtgac agatgagccc gcaagcacca ccataacggt
     2161 atctttaggt atttttatgt tgccgctgag ggttatgtta ttttttatgt aatacgttct
     2221 ccctgctgcg ggtgcacttg tgccgtccca tgaataaggg tctgccgccg ccgctacagg
     2281 cagcgatacg gaacagaata taaatatcgc cgataaaacc gaggcgataa tacgcttttt
     2341 gatcttcatt tgcttttcct ttcagtggtc cgggttgccg tatgttatga tagagccgga
     2401 cacctatcca agataattat gtcatatccg ccgccgtttg tcaatattca tcttgttgtg
     2461 ccgcaaaatg acatttaaat ttcgtcaata cgacaagcgg tggggctttg cgtttgtgca
     2521 tagttacaaa taaatctgcg cgatctgtta ttgacagcac tataaaaaag ggttataatc
     2581 tctatgtctg cgggttatgc cagaataaaa agaaacggta caatatatat gaataaatat
     2641 gtaagaaata tgacacaggg cagtgagctc aggcacatac ttctgttcac tctgccgctg
     2701 cttgcgggaa atctgttcca gcagttttat aatatagtgg actcggtgat agtcggacgt
     2761 tatctcggtc acgaggctct ggcggcagtc ggtgctacag gttcgataac gttcctgttc
     2821 tatacgctgt gcaacggtct ttctgcgggc gcgggtatac tgatagcgca gagcttcggt
     2881 gcgggaaagc atgacgatgt gcggcgattt atcgcgaact cggcttatgc actcggcttt
     2941 gtcggactgg gacttacgct gatctctgtc acggcggcac atcttctttt acagcttctt
     3001 gatacgccgc agaatatcat tgaaagcgct acagactata tgcgcaccgc ctgtgcagga
     3061 actatcgccg ttgccggcta caactggata aattcggtac tgcgtgcgct cggcgactcg
     3121 aaaactccgc tgtacttcct tatagttgca agtgtgctca atgtcggact tgacctgctt
     3181 ttcgtaatgg tattcggtat gggcgttgta ggagcggcgc ttgctacagt tatagcccag
     3241 ggtgtttcgg cagtcggaag catactgttt gcagtaaaga agaatgaata tttccgtatg
     3301 gaaaaagagc atttgaaact gcgcaaggag cattttatgc gctgtatgaa aacaggtacg
     3361 cctatagcgc ttcagaatgc gcttgtttct gtatcgatga tctctctgca gaaaaccgca
     3421 aactcattcg gagatatagt agttgcggcg tacacggcaa caatgcgtgt cgaacagctg
     3481 atacagcagc cgttcaactc actcggcacg gcactgtcta cctttgcggg tcagaatgtc
     3541 ggtgcggcaa agcccgaacg tgtagtcaga ggatatcacc gttcaatgct tatatccacc
     3601 gcattcgggc ttgtcatgct cggtgtgttt gctctgacga gcagatatat tgtcggcttt
     3661 ttcgtcggcg aggagcaggt catcgatatt ggcgcaaagg cacttttatt gtctgcgttc
     3721 ttctatgtcc cgctcggttt tatccataca acaagagggc ttttaaacgg tgcgggcgat
     3781 gtaggctttg cgtttctcaa cggacttgcc gaggtaatag gcagaatagg ctttgcggca
     3841 atacttgtcc atatacccgg tgtcggaatg tgggcggtgt ggactaccac ctgtcttaca
     3901 tgggtactga ccgctgtgat gtgccttatc agatacaagg gcggcaaatg gcgcagaaaa
     3961 tgtcttgtcg atcttgaaga aaacgatcca aaggaggcgg atatatgctg agcaataaaa
     4021 cacactctga aaagaacgat accgttcttc tcgcacacgc cgcgctgctt ttagtgatgt
     4081 cgcttcttgt ggcggtacag ggcgtatttg aaataatcga ctatatgccg cacataatct
     4141 cggcaggctg tgtaaactg
//
LOCUS       DAWAOG010000134         4143 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2759_length_4143_cov_1.819961, whole genome shotgun sequence.
ACCESSION   DAWAOG010000134 DAWAOG010000000
VERSION     DAWAOG010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2759_length_4143_cov_1.819961"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1645..1654
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1994..2003
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3473..3482
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1178 a          981 c          931 g         1023 t
ORIGIN      
        1 caaactgatt gaccacaaag aaaagtattg caagcagtat aatacctttg ataagctgct
       61 ctgcacgcgt ttctttgatg atttttatgc ctttatatat aaaataggca agcaggagaa
      121 tatcaagata gtcaacaagt cccattgtcg caaaaacact gagtatattc tcccagatat
      181 ccgctaaata ctgaatcaac gtgttactcc atttccaaaa ctgcacaacg cagtgaattt
      241 catcgtaaaa tctatataaa tattgtacca ttaaacagtt gcgatttcaa taagcagacg
      301 aaattttttg ttatttttca caaacaaaat cagcccctat gagaacaagg ttcgacatct
      361 gtgagcaaca cagaagttag ctttggcata ccgcagcttt atgagattta ctaaaaaaat
      421 tcttattttt ttcactttta gaggtttaca ttcagagacg attatgatat aatactaagt
      481 tgaaagcata cgtcttgata tggcgatatg caaaaacaaa ggaggacgat tcccatggga
      541 agacaaaaac ttactatgga cggtaacaac gccgcaggtc acgtgtctta tgcgtttact
      601 gacgttgcag cgatttaccc cattacacca tcttcagtta tggctgaagt aacagattcc
      661 tggtcaacag ccggaagaaa gaacattttc ggacaggaag taaaggtagt tgagatgcag
      721 tcagaagccg gtgcagcagg cgcagttcac ggctctcttg cagcaggtgc atgtactaca
      781 acttacacag cgtcacaggg cttactgctc atgatcccca atatgtacaa gatcgcaggt
      841 gaattactgc ccagcgttat tcatgtttcc gctcgtgctc ttgcaacaca cgcactttct
      901 atcttcggcg accacagcga tatctacgct tgccgtcaga caggttatgc tatgctctgc
      961 tccaccaacc ctcaggaagt tatggatctc ggtgctgtag ctcacctttc aacatacgaa
     1021 gcaagagttc ctttccttca cttcttcgac ggtttcagaa catcacacga gatccagaag
     1081 attgaagtat gggactatga aacactcgac aagatgctcg accacgaagc tgtagacgct
     1141 ttcagaaaca gagctctcaa tcctgagaag cccgttctgc acggcacagc tcagaacccc
     1201 gacatcttct tccaggcaag agaagcaagc aacaagtact atgaagctac acccgctatc
     1261 gtacagaagt acatggataa ggtaaatgct gagatcggta cagattacaa gctgttcaac
     1321 tactacggtg ctcccgatgc tgagcaggta atcattgcta tgggctctgt ttgcgatact
     1381 atcgaagaaa caatcgacta tatgttagct cagggcaaga aggtaggtct tgttaaggta
     1441 agactttaca gacccttcag tgttgatgct ttcgtatcgg ctatccccga aacagttaag
     1501 aagatcacag ttcttgacag aacaaaggaa cccggcgcac tcggcgaacc cttatacctc
     1561 gacgttgttg cagctctcaa gcaggctaac aagttccagg atgtacccgt attcacaggt
     1621 cgttacggtc ttggttctaa ggatnnnnnn nnnnaccccg ctcagatcat tgcagtttac
     1681 aacaacacag aaaagcccag attcacaatc ggtatcgagg atgatgttac aggtctttca
     1741 ctgcctatcg gtgagaaccc caacacaact cccgagggta cgacctgctg taagttctgg
     1801 ggtcttggtt cagatggtac tgtcggtgct aacaagaact ctatcaagat catcggcgac
     1861 cacacagata tgtatgctca ggcatacttc gcatatgact caaagaagtc gggcggtgtt
     1921 acaatttctc accttcgttt cggtaagtca cctatcaagt caacttactt tgtaaacaag
     1981 gctaactttg tagnnnnnnn nnnccacaac ccctcataca tcgacaagta cgatatggtt
     2041 gaggacgttg tacccggcgg ttcattcctg ctcaactgcc agtggacagt tgacgagctt
     2101 gacgagaagc tccccgctcc cgttaaggca tacattgcta agaacaacat caacttctat
     2161 atcatcaatg ctatcaaggt agctaaggag atcggtcttg gcaacaagac aaacactgta
     2221 ttacagtctg cattcttctc aatcgctaac atcatccccg ctgaagatgc tatcgaatac
     2281 atgaagaagg ctgcttacaa gtcattcgct aagaagggtg acgatatcgt taacatgaac
     2341 tacgccgcta tcgaaaaggg tgcaggcgag gttatcaagg ttgacgttcc cgcatcatgg
     2401 gctgacgctg agggtacact tcctgtacac acagctacag gcgacagaaa ggatctcgtt
     2461 gacttcgtta acaatatcct tatccccgtt aacgctcaga gaggcgacaa gctccccgta
     2521 tctacatttg taggcatggc tgacggtaca ttcccccagg gctctgcagc ttacgagaag
     2581 cgtggtattg cagtagacgt tcccgagtgg attcccgaga actgtatcga gtgtaaccag
     2641 tgttcattcg tttgtcctca cgctgtaatc cgtcctgtaa tcctctcaga ggaagaagct
     2701 gcaaaggctc ctgcaggcgc taagatgaag gacgctatgg gtcttcccgg caagaagttc
     2761 gctatggtaa cttctgttct cgactgcaca ggctgcggcg tttgcgctca ggtttgcccc
     2821 tctaagaaca aggcactcgt tatgaagtca cttgattctc agatgaacga acagcccatg
     2881 tttgactacg ctgttaagga agtagcagac aagcccgagg ttcacgagaa gttcaaggaa
     2941 acaacagtta agggttcaca gttcaagcag cccctgctcg aattctcagg cgcttgcgca
     3001 ggctgtggtg aaactcctta cgctaagctc atcacacagc tcttcggtga cagaatgtac
     3061 attgccaacg ctacaggctg ttcatcaatc tggggcggct ctgctccctc aactccttac
     3121 acagtaaata aggaaggcaa gggtcccgct tggggcaact cactgttcga ggataatgct
     3181 gagtacggtc ttggtatgtt catcgctcag gatacattaa gacacagagc acagggtcag
     3241 ttaaaggcta tcgctgaaaa gactgacaac gctgacgtta aggctaagat cgatacatac
     3301 ttcgagacag ctgacgacgg taaggcaaac gctgctgcta ctaaggagct cgttgcagct
     3361 cttgaagctc ttaactgcga ctgcgctgaa aagacagcag ttctcaaggc taagaactac
     3421 ctcgctaaga agtccaactg gatattcggc ggcgacggct gggcatacga tannnnnnnn
     3481 nncagaggtt tactccaaca caggcggaca ggcttctaag gctacaaaca tcggtgctgt
     3541 tgcacagttc gcagcaggcg gtaaggatgt taagaagaag gatctctccg gtatcgctat
     3601 gaagtacggt tatgtatatg ttgctcagat ctcaatgggt gcagatatga accagtgctt
     3661 aaaggcaatc gccgaagctg aagcttataa cggtccttca ctgatcattg cttacgctcc
     3721 ttgtataaac cacggtataa agggtggtct tacaatcgct cagaaggtag aaaaggaagc
     3781 tgtacaggca ggctactggc atctgttcag atttaacccc gctgctcccg caggaacaaa
     3841 tccgttcact ctcgacagca aggctcctac aggcgattac aaggaattca tgatgagaga
     3901 agttcgttac aactctctta tgcgtgctaa ccccgaaaga gctaacgtac tgttcgacaa
     3961 ggcagttgct aactccaaga agcgttatga agatcttgta cgtcttgttg aatactacaa
     4021 gaaggaatac tgcggcgaat aatccgcacg aaataatcta aaagcccaaa aattttgaat
     4081 agtcggaata gaataggttt ttacgcccct ccggtaaaac cggaggggcg gcctttttgc
     4141 ttg
//
LOCUS       DAWAOG010000135         4141 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2762_length_4141_cov_2.173030, whole genome shotgun sequence.
ACCESSION   DAWAOG010000135 DAWAOG010000000
VERSION     DAWAOG010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2762_length_4141_cov_2.173030"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1552..1561
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1260 a          821 c         1092 g          958 t
ORIGIN      
        1 gccaaatccg ttcccgatga gcatttacgg taatatagca tacggcccga gagtacacgg
       61 cataaagagc aaggcaaagc ttgacaggat agttgaggag agccttatcg gtgcggctgt
      121 ttttgacgag gtaaaggaca gactgcataa gtccgctctc agcctttcgg gcggtcagca
      181 gcagagaata tgcatagcaa gagcgatcgc ggttcagccg gaaataatct tgatggacga
      241 accgacatct gcgcttgacc ccgtatctac attgaaaatc gaagagctta tgaacacgct
      301 gaaggaaaag tacaccgttg ctatcgtaac tcacaatatg cagcaggcaa ccagaatttc
      361 ggactacacc gcatttttcc ttgtaggcga aatggttgag tatgcaccta ccgaagagtt
      421 gttctcacga ccccgagata agcgtacaga ggattatatc acgggacgtt tcggttgatt
      481 ttcggcatta tatgtgatat tatattagaa agggcaaaga aaaatgagag aaacatttga
      541 gcataagctc ggcaagctga ataacgatct tatccgtatg ggcggtatga tagaggaggc
      601 tatagcgtca tctatcacag cactgcgtga cagcgatact gagcttgcaa aaaaagtagt
      661 agacggcgac agacaggtag atgaggaaga gaagaacatc gaaagtcagt gcctgtcgat
      721 aatacttcgt gaacagcctg tcgcaaggga tctgcgtaag gtttctgcgg cactgaaaat
      781 ggtgaccgat atggagcgta tcgccgataa tgccgcagat attgccgaga tcagccttac
      841 agccgatgta tctaccatta tgcatatagt aaaagagata cagactatga gcgaaaaggt
      901 cataataatg gtatctcact ctatcgactc atttgtaact acagatatga cgcttgcaaa
      961 tgaaacgata aaggcggacg atgaggtcga tgaactgttc atttcaataa gaaaccgtat
     1021 agtcgagctt ataaagagcg agcattgcag cggcgaggta gcggtcgatg ccctgatgat
     1081 agtaaagtat cttgaaagaa tagccgacca tgctgtaaat atctgcgagt gggtggagtt
     1141 cttcaagacg ggtgaatata agaatacccg tattatataa actttggagg ctggaataat
     1201 ggctgaacgt gtctacataa ccgaggacga tgacagtata agagaattgg taacagtggc
     1261 tctttccgct tactcatacg aggtcgagag ctttgtaagt gcggaggatt gtcttgcggc
     1321 tgtagcaaag caagtgccgg atatattcct gtttgacata atgctgccgg gtatagacgg
     1381 tgtacaggcg gtcaagctcc ttcgcgaaaa ggaagaaaca aaggatactc cgatactgat
     1441 gcttacagca aaatcagccg agatagacaa ggtgttcggg cttgaaaacg gagcagacga
     1501 ttatcttaca aagccgttcg gcgttatgga gcttgccgca agaataaagg cnnnnnnnnn
     1561 ngcattactc agacgtgcgg gcagaacatc tgccgaaagc gcatcggata agctgaccgc
     1621 aggcgggatc acggtgaata cttctctgcg tgaggttagc cgtgacggac agcaggtcga
     1681 acttacgctg aaagagtatg agctgttgct gtatcttctt aagaaccgtt caagagttgt
     1741 cagccgcgag gagcttctga caaaggtatg gggcatagat tttgtcggtg aaacgcgaac
     1801 gcttgatatg cacataggaa cgctaaggaa aaaactcagc gatgatgccg ataacgcaca
     1861 tcttatcaag acggtgagag gtatcggcta cagatttata ggagaataac gggaggacgt
     1921 acagatgaaa aaagcactta tagtacgttt tcttattgtg atagcgatag ttatgtcggt
     1981 gtgtattgtt tctgcgaccg cactgctttc aagtacggaa cagaatgtca gaaaaagcga
     2041 tatgctgacc tctctcgggc ttatccgcga gatatatgag gacgaaccaa gtcacgacta
     2101 tgatctcgct tacagattaa gctcacttat cggcaatgcc agagtgagct ttatcaataa
     2161 ggacggcact gtatttgtag atacctatat agacggtgag cctgacgaca atcacaacag
     2221 ccgtgaggag ataaagcaag ccgagcttac aggaagcggg gtatcggtaa gatattcaaa
     2281 gacactcggg aaaaatcaga tatatgccgc tatgaaggta aaggacgacg atataatccg
     2341 tataagctat aacataaaca gctatatgga ttttgcttcg atgttcattg cacccatgct
     2401 tatagccgcc gcaataggta tagttgccgg acttatagcg gtaagcagtg tcgcacggca
     2461 gataatgagg cctatcaaca gtatatctgc ggctatgggc aagatgaagg atgtaagcaa
     2521 tgaaaacgcc gccgagctcg aagagatacc ctcgcacaag taccctgagc ttgacgagtt
     2581 cgttacgatg ttcaacttta tgcgcaagag catacaggat aatatgaaaa cgcttgaaaa
     2641 cgagcgtgaa aaggaacgct tcatacttga gagcttacag gacggtgtta tactctatga
     2701 taacgacctt tccgtgatat ctatcaatcg cgcgggactg aagctgctcg gctcttccaa
     2761 ggacagccgt atcctgacag ttcccgagct tgcaagaaag ccggagataa taagcggggc
     2821 tgaaaaggcg gcaaacgagg ataagacggt aaatattgaa agcacagttg acggcaggat
     2881 attgctcgtt cagatattcc ctgtttcaaa gagcggaata gatgctatgc atggtggact
     2941 tatggttatc cgtgacgtta ccgacaagcg tatgtccgaa cagctgaaag ccgatttctt
     3001 cgccaacgca ggacacgagc tgaaaactcc gttgacggcg atctcgggct ttgcggagct
     3061 tcttgaaaac ggacttgtgc ctaaggataa gaaggactat tacatcggaa aaattcttac
     3121 cgaagccaaa agaatgtcaa ccataatcag cgatatcctt gaaatatcct cgcttgaaaa
     3181 caagacgaca gccgaagaga taaccgactg taagctgtcc gagatatgcg ccgcagtaaa
     3241 ggaatgcctg gagccgtccg catcggcaaa gaatgtgacg atagaactgt cgggtgacgg
     3301 ctttacagta cacgccgcat acaagcacat ttacgagctt gtcgaaaaca tagtcagcaa
     3361 tgcggtgaaa tacaataagg acggcggtaa ggtagagata acgcttcaca atgtcgggga
     3421 gataggctgc atctatgtaa aggataacgg cataggaata cctaaggagt cgcttaaccg
     3481 tgtatttgag cgtttctaca gagtagataa aggcaggagt acaaaagagg gcagtacggg
     3541 actagggctc tctatagtaa agcatatcgt taactgctac ggcggaacgg tgacattgga
     3601 cagtgagctc ggcaagggaa cgctcgtctg cgtaagactg ccgatcgtga agaatataat
     3661 ggagtaatct cttagtttat agaaaaattt ctatctttgc taaaaagggt tgaggggcgt
     3721 agcccccaac agggactagg ggcggtagcc ccagtaaaat agccccttcc cgaggggaag
     3781 gggtttgggg ttagggtttg agaataagct gttttcttaa aagcagactt tttctataaa
     3841 taggtaagct tcttaattca tatttttccg cttgacacgg cgttgcattt atgatacact
     3901 aagcatatag taaaatatag atgtaaagac cgtgtacggc aagagtaccc ggaaaatgaa
     3961 gtcttacaga gagccgtgtc aggtgaaaga cggcagacgg attttcggcg aagtgcggct
     4021 gtgcggatat ttttatccgg gtcggcagag ggaaaatatt ttacaagtat ctctgctcgt
     4081 gtagtctgcg ttaaagacat aaagggagcg caagctccga agcgaagttg aaccgtggta
     4141 c
//
LOCUS       DAWAOG010000136         4118 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2783_length_4118_cov_2.082944, whole genome shotgun sequence.
ACCESSION   DAWAOG010000136 DAWAOG010000000
VERSION     DAWAOG010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2783_length_4118_cov_2.082944"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    484..493
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1034..1043
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1480..1489
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2352..2361
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1151 a          891 c         1016 g         1020 t
ORIGIN      
        1 atgttcagga ctggggactt tcaagaaaca gatattgggg tactcctctt aatatctggg
       61 agtgcgagtg cggtcacaag cacgctatag gcagtatcga ggagcttaag tcaatgtcgg
      121 ataactgccc cgatgatata gagcttcacc gcccttatat cgatgcggtt acgataaagt
      181 gtcctcactg cggtaagcag atgaagagag tgcccgaggt tatcgactgc tggttcgaca
      241 gcggttctat gcctttcgca cagcaccatt atccttttga gaataaggaa aagtttgaac
      301 agcagttccc cgcagacttc atctctgagg ctgttgacca gacaagaggc tggttctact
      361 cgctgcttgc tatatcaacg cttctgttca acaaagcgcc atataagaac gttatcgttc
      421 tcggtcttgt gcttgataag gacggtcaga agatgtcaaa gtcaaagggc aacgctgtag
      481 accnnnnnnn nnnacggcgc agacgctatc cgttggtact tctacacgaa ctcggctccg
      541 tggcttccta acagattcca cgcaaaggct gtaaacgagg gtcagagaaa gtatatctct
      601 actctgtgga acacctatgc gttctacgtt ctgtacgcta atatagataa ctttgacgct
      661 acaaagtaca cgctcgatgt tgacaagctc ggcgttatgg ataagtggct gttatcaaga
      721 cttaacaccg ttgttggtgc ggttgacgat aaccttgcaa actaccggat ccccgaagcc
      781 gcaagagcat tacaggattt cgttgatgag atgagtaact ggtatgtccg ccgcagcaga
      841 gagcgcttct ggtcgaagga gcttacgcag gataagataa acgcttacat gacgctgtac
      901 acagcgcttg taactatcgc aaagacatct gctcctatga ctcctttcgt atgcgatgat
      961 atctacagaa accttgtttg ctcacttgat aagaacgctc ctgtaagtgt acatctgtgc
     1021 gacttcccta cggnnnnnnn nnncagttct gacagttgta acgctcggac gtgccgcaag
     1081 aaatgccgct aacatcaaga acagacagcc tatatcaaag ataatggtaa agggcgacaa
     1141 gacgcttgag cctatgtatg cggatatcgt aaaggacgag ctgaacatca aggagatcag
     1201 ctttatagat aatacagacc acttcacaag ctacaccttc aagccccagc tcaagacgct
     1261 cggtgctcgt ttcggcaagc agataggcga ggtaagaaca aagctcgctg aggttgacgg
     1321 tcagaaggct atgaacgaga tccgtgaaac aggcgctatg acgctgacgc tttcaacagg
     1381 cgatgttcag atcgcaaagg acgacctgct gatcgatgag catcagaagg agggctatgc
     1441 ggtagtgacc gacagaggct acacggtagt tcttgacacn nnnnnnnnna agtaagatcc
     1501 agacaatgcg taaggacgca ggctttgagg ttatggatca catcgttatc tactgcgacg
     1561 gcagtgacaa ggtagctgag atactgaccc gcaacaagga cagcatctca gaggatacgc
     1621 ttgccgatga gatagttacc ggcaagactg acggatttac aaaggagcag gatataaacg
     1681 gcgagagcgt aactctcggt gtcaagaaaa tatgagcttc aagcagacat tgacgagcct
     1741 tttcggacat aacgcagtcc gcaaggctct gatatgggtg ctggtcgttg ctgttgctgt
     1801 tctggtcgtg gtaacgcaat tcgcgttccc ggtacaggta cagtatgggt atacacggct
     1861 tcacacctac agcggacaga gctttaacaa tacgataaaa aacgtatacg ataagctcaa
     1921 agacagcact caccttgaca gtgggtctac aagcaagctg ctcagcgaca cggattttga
     1981 tatgtcaaag cccgaaaact atctgcgtgt tgagatggtg ttcacattca ccaatatagg
     2041 aatgtacaag atagacaaca ttcagttttc gatcgacgac ataccgtatg aaaagcaggc
     2101 gtttgtgctg aaagaaacaa atatagcttc gatcgaccgc ttcaacaaca gtaaggtgtc
     2161 gctcatattt gttatcgaca ggtcgggact tacggacgag aaaataacaa aggctgtaag
     2221 ctcgcttaag ataagctata agtttgaccg ccccgagctt ttctcatcgg gcggcgagat
     2281 aacactgcct gaaactgtaa ttctgccgtt tgatgaagcg gtggaggagt agttatggcg
     2341 ggaattttta annnnnnnnn ngataccgca gaagcagaaa ggcgtgctgt gcattatcag
     2401 ctccgccttt tgctttgcgt ttatgggagc gtttgtaaga ctggcgggtg atctgccgtc
     2461 ggtgcaaaag agctttttca gaaaccttgt cgcgtttata tttgcggcag tgatactgat
     2521 aagacagaaa ggctcttttc tgccgcatag caaaagcaat atcggcgcac tgctgatacg
     2581 ctcggtatgc ggtacgctcg gaatactgtg caacttctat gcggtggatc atttagcgtt
     2641 atccgatgcg gcaatgctta acaaaatgtc gcggttcttt gttatcgtat tttcggcgtt
     2701 gttcttaaag gaacggacta atttagtaca gacgctcggc gtaatgatcg cgtttgcggg
     2761 aagtctgctt ataataaagc cgagctttgc aaatctggag cttgtgccgt cactgctggg
     2821 atttctcggc ggtatgggcg cgggcgcggc ttatacggct gtaaggtggc tcgggataag
     2881 gggcgagaag ggaccgtata tcgtgttctt cttctcgggc ttctcctgcc ttgtgacact
     2941 gccgttttta ctgttcgatt ttcacgttat gacgtggcag cagtggctta cactgctagg
     3001 cgcgggactt gcggcggcgg gcggacagtt cagcataact gcggcgtata aattcgcccc
     3061 ggcaaaggaa atatcggtat ccgattattc gcagataata ttctcggcac tgctcgggtt
     3121 tatgatgttc tcacagctgc cggacatatg gagctttatc ggatatgtgg taatatgcgg
     3181 cgtgggcgtt gcgatgttta tatacaacaa ccgcaaggat aaaaaatccg tgacagaaaa
     3241 ttaagaactg ttaacagaaa atattgacac cggtgtactg atgtggtata ctgactaaag
     3301 atacacaata tcggaatgtg tgtcacttat tgaaaaaaga ggtatgtaat tatgaaaaca
     3361 gcagcacagg tatttataat catcggtatg gtttgcggct tctgggtaat acttccgctt
     3421 atcttcggcg gcatcgcatt aagccagatg agcaagggtc agcgtccctc aacgggtatg
     3481 agcgtatgcg ttcttttatt ctgcagcttt ataggcggta ttcttctgct ttgcgcaaag
     3541 gacgaggatt tttacaaccc caacatgaac agcggctata accccaacat gaataatggc
     3601 tacaacccca acatgaacaa cagctacaac gccaacatga ataatggtta taacgccaat
     3661 gtaaataacg gttacaacgc caacacgaac aacggttaca acgccaatgt aaataacggc
     3721 tataacaatg caaattatgc cgctcccaac aactacaaca ataattatgc tacaccggac
     3781 aacaatttca ataataacaa caataacggc gtacagcagt aatcgaaaca gtacatagca
     3841 catgacggca gagcacaggc tctgccgttt ttgcgttgta tatcagcgta caacatcatt
     3901 tcgccgtatt tacatacttg acagcattta gcagtcgtgc tatactgtat aaagaatatt
     3961 aaaacgttga atactgacgg gaaatggtgg gagatatgtg taaggcgtta gacaccaatt
     4021 tgtgtaaaat agagctaaat ataagcgcct ctgtaagttg tgcacaatat ctctgcgtaa
     4081 atcaattcaa tacggtgatt aaggcatagt ccgcgagg
//
LOCUS       DAWAOG010000137         4051 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2856_length_4051_cov_2.121371, whole genome shotgun sequence.
ACCESSION   DAWAOG010000137 DAWAOG010000000
VERSION     DAWAOG010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2856_length_4051_cov_2.121371"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    232..282
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1583..1592
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          966 a          969 c          892 g         1163 t
ORIGIN      
        1 gcgatacagc acatacgact gatccttggc gttgcttgta ccgtcagcgt ttatcgcttt
       61 ctttaacagc catctgccgc ttttttcgtc atagcagctg cggacataat gaccggtcgc
      121 aatgtggttt ataccatggt tttttactgc gtcaagcaat gcaccgaact tgatgatctc
      181 gttacattca acacaagggt tcggggtcct gccggacaga tagctctgta cnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaacaaatc cctcatatca
      301 agcagatagt gcggtacgcc gaactttgcc gctatagcct ttgcgtcctt gatgtcgctt
      361 gccgaaccgc aaagtcctgt atcgtcagcc gcccggctgt cgtgaagctt cagcgtgtag
      421 ccgctgacat catatttatc ctttaacagt accagagcta ccgagctgtc tactccgccg
      481 ctcatcgcta ttgccaattt ttccataata ttatccttta tctttaagtc ttaactgtac
      541 gattgcaccg taatgatgta tgtcaaattc gggcggcgca tttgtttcca ggagcttcaa
      601 gtgctctttt tcccacgaat ttatcttttc ttcggacagt gaagcgccta tgcctcgaca
      661 ggctttcatt ctgccgttcc agctctctcg tgtgaaatgc acgtcgagcg gatattcttc
      721 actgtaaact ttatcaaaat aagcgtcgta acactcaggt atatgtatcg ggtgcatcgt
      781 ttcgcccttg ccgctccagt cggggttata tttcagtaca agctcctcgc tcatacctgc
      841 aatcttatct tcaaacggca gccacgccat atagagcacg acaaggcttc cgctgctttt
      901 cagcatactc atgaacttcg gtatcagatt ttcatggtcg aaataccaaa agcactggca
      961 agcggttatt acgtcaaatg aattgtccat gaacgacaat ttctcggctg gcgaagcaaa
     1021 atattctata tccgtaccct gtgacaacag ttttgcctgc tctatctgct ccggtgaaat
     1081 atccacgcct gtccactttg cgccgaagcg gagcatatta cgcggaatta cgcccgttcc
     1141 cgttcctata tcaagcacac tctgtccaga cacacaaagt cctctgtcgg ttattctgcg
     1201 gtaaaactcc tcagggtata tatcacggta cttagcataa tcctgcgata ttctgcccca
     1261 gtcgaaggct ttaccattgt cgatattgct gtcggttata ttcatagaaa ttcctttaca
     1321 aattaccgcc gctgtgtttt tcacggcggc ggtgctgttg ctataattta cttttcttcg
     1381 tttcccgcaa gctgtgaggg gtgctttttg aagaagtcaa ctctcggctc aaggaactta
     1441 agcttgtgct cgctctcacc cttgcagaag tagtccattc cttcaaagac atgatcccag
     1501 ctccagaaat cctcaccgaa gccgaggtac tcacggagca tctctgtacc ctcgggagcg
     1561 agggggtgca ggagaagtgc cgnnnnnnnn nngttcttat atagtggaac acgttgataa
     1621 gcgtcttttt acgaagctcg ttatcatctg ccgcatcagc ctcacggctg agctttgacc
     1681 ataccttgct ggactttctt acaagcgagt caagtacata agtcacctga tggaactcaa
     1741 acttatacat ataacgctca tattcgagga tcgccttttc gcactcggcg ataacatcgg
     1801 catcgggtgt gcctacaggc atcttgccgt caaaatactt ctgagcgtaa tagaagcagg
     1861 agcggatcgc tctgttgaat acgtttgaaa gcaggaagcc ctcttttaca acggggtcgt
     1921 tgtcctcagg tctagcatcg ggattgtagg gcttaggctg gaagctgacg ctccttgtgc
     1981 cgagtccaag tccgagccag tgcatacgga gctgttctgc cgtatagtag ttaagcaggt
     2041 ctgccgccat gggaggcttt atagagccgc tgctgcttgc cttcttgtca aggaacagaa
     2101 tgtggttgtt ggcaacgagg ataggcagct gcatctcgcc gtcctcaggg tctgaggtta
     2161 tctcaccctt tttcagtgcc ataaacattg ccatttcggc aacaccgtag aagtagatgt
     2221 tatccgaacc gatgaactga tatacctgtg cgtcctttga gcaccagtaa tccttgtagt
     2281 gctccatatc tatacccttg ctcttgagat aagcgcaggt gaatgagata ggtgcccaca
     2341 gcgactcagg ccatacccat acggtaagtc ccttttcatc ttcaaggtcg ggtgctttta
     2401 cgccccactc aatgttacct gtaagtctga acggaacaag cgtcttgcct gttctgaaac
     2461 gcaggccgtg gtgcgccatt atctcggtag cggtatcgcg ctcatcaagg gtgtcgaagc
     2521 atatcgtgaa tgacggcttc ttggggtcat cggtcagtgt atgatgtgca agaagatcct
     2581 ttatcttttc atagtcttca cgcagttcct ttttgatatg gacaacgggc ggttcaagga
     2641 actcaccgat agtttttgaa acaagcgaac ggacgttagg catcttcttt attctgtcaa
     2701 cgtattcgcc gaggagcttt gtgtactcgg taaggcggaa gtaccagttc acaacgtcac
     2761 gcattacggg ggtttcgcct gtgagcgtgc tcttggggtc gataaggctt gacggcatat
     2821 actgatgtcc gagatcgcac tcgtccgcat agcccttttc ggactgacag ccgagaacgg
     2881 ggcatttgcc tattacctgt ctgccgttta agaaaacgcc tgccttttcg tcatagaact
     2941 gtgaggtggt gatcttttca agctgaccgt tttcatacag tcttctgatg aaccggtcgg
     3001 acacggtatt gtgtatctcg gcaggctcgt caagtgccga tgcgccgaaa agatcaaggc
     3061 ttatgtcgta atctctgagc gttttttcct ggctctcatg gttctttctg acaaaatctt
     3121 caatagtacc atcaaactct ccgctttccc tgagctttct gtagctttcg gctatgggtg
     3181 agccatagca atccgtaccg gatacgaaaa tcacgttatc cttaccgata cggtcacgca
     3241 gaaatcttgc ataggtatcg gcaaagacga acacaccgcc gacatgaccg aaatgaagct
     3301 gtttattgcc gtagggcata ccgcctgtta tcaccgctct tttcgggaaa gacggtctgt
     3361 tatcctttga cggcttattg cttttataat tattgttatt tttgttgctg ttttccaaat
     3421 ttcaacactc ctcgatgaag ttttatacgg tttattgtat cacatatcgt ctgaaaatgc
     3481 aataaattta tccgattttt gttgtatcgg cagattatgt tccgaagttt aaaatgtaag
     3541 tcttgcggca catagctatt acaacgcaaa tctgcgaaaa cataggcaaa tcggcgctat
     3601 tttgcttgat ttttcagaac gaatgtgcta tactgattat attctatatc taagattaaa
     3661 cgatttcaca acatttagcg tttcaagaaa aaatctcacg aagggagact ttatcattgg
     3721 aaaataaaag cggaagaaaa aacgttgcct tttgtgtagg cgatcttgaa ggcagtaaaa
     3781 tgttattgca gggtttttgc aatgtgttgg gcgacaatcg gataaacgtt catatcttca
     3841 actgctgtac ctgtttctat gatggcgcac cgcacaatat aggtcagaca aacatcttca
     3901 atcttcctaa ttatgatatt atcgatatgc tcattatcgc accgttctat ctaagcagca
     3961 gcgaagaagt gatacggcat actatcgaac gtgcggtaga gaaaaacgtg cctgtactca
     4021 ctatcggaaa acagtatgat taccccaact g
//
LOCUS       DAWAOG010000138         4018 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2887_length_4018_cov_2.212970, whole genome shotgun sequence.
ACCESSION   DAWAOG010000138 DAWAOG010000000
VERSION     DAWAOG010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2887_length_4018_cov_2.212970"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3263..3272
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          963 a          981 c          823 g         1241 t
ORIGIN      
        1 tctgttacaa gtgaaaagcc gttcactgac tgatcggcac ttccgccgcc aaatccgccg
       61 acagatactt ccgccttgac cgacataaac ggaagaagca gcgcaagaac tatcagaaca
      121 cagataattc ccataagatt tttaagtata aattctttat tgagtttcat aacaatctcc
      181 ttttgaatta aagcgtttcc ctgtaatatt cctgttcctc acatattact atatttccgt
      241 cacctatgag cgtagaagct gaaatttcaa cacccgaagc aaaattgaaa tatataacgt
      301 atgtgccgtc atcatatgta gcgaatgtgc catcatccct gagcgttata taatcgccgt
      361 agttttcata cagataattg ctgaaagctc catatgtcac ataaccgtca aaatcggtaa
      421 agaaagatat tataggacac tcaaccataa tcgccttgcc tcgtcctaag atcgggtggt
      481 cttgaataaa gtcttttctg caatagtaga tatcaagacc gtccttttcg tagcaggcat
      541 aatatccggt ataaatgctg gctgtgatcc cccatacatc ggcaaaatcg ttatcaaaat
      601 aatactgtat atagtccgga agtgctatag ttccattggt aacagcagga gcttcagcct
      661 gtgtttcagc ggctgtggtt tcaggtgcgg gagttgtagc cgctgttgtc tgtgcggtcg
      721 tttcaggtgc gggagttgta gcagccgttg tagttgtcgt ttcgggagca accgttgtct
      781 gtgccgatgt ggtatcaggt gcttctgacg gttcatcatc tgaaatatcg ggtacggaat
      841 aatccgctcc gtccttgtcg tagatgccgt taaaaagccc actgccgttt gcgtccgcga
      901 caattgacat ctgcttttcg ttcacgtttg cggtgcaggt gtcactgtag gtctgaccgc
      961 ctgcaacgat cgtaagtatc agcttaccgt ccgcgatatc gtatgtaccc tcatcggact
     1021 gtgccgtaac agtaccgtct gccgccgtct gcttgtatga cagctcctta ttgtccttga
     1081 acctcagctc atatgtgctg tcgcctgctt tcttgtacca ggcaccgtaa atgtttaccg
     1141 tgttcagctc catcgtggcg ctgaccgtgg gctgattttc ccctttctga ccgcttatta
     1201 tgaaataagc cgccaatgcg acaacgataa ccgccgctgc aacgcacagc gcgattatgc
     1261 tgttcttgtt agatgttttc tgttccattt ttactcctcc tcgtttttct tcttcagaag
     1321 ttttcttata gttctttccg agtagttgta ctcggtagct atattattga gcgaatccga
     1381 tgtaccgtca tagatccgcg ttgcctcacg cgttacatat tcctttgaat agagccgtgt
     1441 aggaaagtta atttgactgc ccttgagctg ttgatacagc tttacggtat tttcaaagcc
     1501 gatagtttcg catatcgaac cgtatacgcc gttcagcaat tctgccttgt tatccttttt
     1561 atccatagca actcccgcaa aaattgtaat tgtatgtatt tttttaatta tactactgta
     1621 cagtgtgtct tttcttctgc ccttttccac ggaaaacagc attgaaaagt atcttgcaat
     1681 gttcctttgc aagtatatta aaacacggtt tgtcacatta tttatgtata aaatgaaaaa
     1741 caaacggaaa aaggcgcaaa aaaatcccga ccttttaaag gtcgggacag actttgatat
     1801 gtatttatca ctctgtaagc tgttcaagct cggcagccat tgactttatc tgcttcacca
     1861 tcgagtcgag agtctgcgtt cccgcagagt tctcctctat agccgccgca acgtgctgaa
     1921 gagcgtcact gttaccggtg atattatggc ttaccgtgcc aagctctctt gttaccgttt
     1981 cgctgttagc aaagacattt gatataacgc tgttcatatc ctctatgttc tgcgttacct
     2041 ccttgtttga agcaccgata tgctcggctg agcggcggat agtttccata tcggaaacgc
     2101 cctctcttgt aagtgccgcg ttgtgcttca ttgcatctgc cgctttttcc actccgtttg
     2161 ttaccgcaga aatgatatcg cctatttctt ctgccgccgt ggctgtcttt tcggaaagat
     2221 ttttgatctc gtctgcgact accgcaaagc ccgagccggc ttctcccgcg tgactggctt
     2281 caattgacgc gtttatcgca agtatatttg tctgggtcga aatgtcggaa ataacatttg
     2341 atatagccgc tatcttcttg gactgcgccg caaggctcag tatcatatcg caggtatcat
     2401 cggtacttgc gcttatcttt tccatgctct gcgacgcggc ggttatctta ttgtcattat
     2461 cggcggttat ctcgttcgcc ttttctgtaa gacctgctat tttgccgctc atctccgaaa
     2521 gactgcgcag cctctcggat atctctgtta tgttctgctc gatcgatttt atggattccg
     2581 cattcgtatc gctgtcgcgc ataacgttca tcgtttcgcc ggatatgctg ctgttggctt
     2641 cggcagacgc tgaggatacc ttttcgagct cgtttaccat atctgccatc gagccggata
     2701 tctcaagggt ctttttatgt atctccttct gcttttcagc acccataagg cttccgagca
     2761 ttatcgcagt acgtctgcaa agcacataga ttattgccga tacggcaata agcactatgg
     2821 ttctgggcat tatcagatag gttatgagct gagttatgtt ttctacatta tgatcctcga
     2881 caaatccgca gataaagcag acatactgcg cacccgccgt accgagcacc gtgaatatta
     2941 ttgtgaacat actgagcttt gtcgagaaga acaggctcgc tatagctata ggatatacat
     3001 aaaggatcac aacgtggtat gcaagtacac acgcaagcac tgttgtgaac aatacggcac
     3061 aggctacggt aaagtatttt acccagccct tttggatctt aaggatattt gtcaccagtg
     3121 taggcaggaa cagcagcacc tcgcctatta taaacgcgac caccattgtg ggcacctgaa
     3181 ccttgaatat ccctacaata tcaagtatca gaaccagcac aaacagtatg cctgtgaccc
     3241 tcattacgct tgccgcggcc ttnnnnnnnn nnctcatttt gctttaataa ttccatttct
     3301 ggttttcacc gctttcatat atttaatttc cccttattaa aaatcatttc ttctcttttg
     3361 catgcgctga ctttatcatt ataagcgtac tcctgaactg ctcactgagc atatcggaaa
     3421 cttccttatc agtgaacaga cagatattcg acgacagcaa tacgcctatg ccgtaagtga
     3481 atatccatac ctgcctgaaa agcatcattg cgtcctcctc ggacaaagcg taatcgtgca
     3541 ttatatattc tatacactta tcggcagtca cgccgagcaa cgatgcgaac atttccttga
     3601 aattgaagct gtgatcctgc tcactcataa acagaagcct gtaaagctta ggcttttcct
     3661 tggcaaatgt gagcatttgc attccgacct gcttgaatgc cggcatatcc gagcttcccg
     3721 tctttgcgta ttcgctgaac aattccatag ccgattcatg gacagcccgt tcgagctctt
     3781 ccatatttct gaacgcagta aagataggtc gtgtggaagt acccatcgct tcggcaagac
     3841 ttcttgcgtt gagcatctcc gctccgttct cggtaactat cccaagcgct atttcggtta
     3901 tttcttctct tgtatatttt gccctgggtg gcataatatc acctcgtgat atggattttt
     3961 atgtaacact cgttgcacaa tgctcatatt ataccataaa tcaggtcggt gtaaaccg
//
LOCUS       DAWAOG010000139         3973 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2917_length_3973_cov_2.586524, whole genome shotgun sequence.
ACCESSION   DAWAOG010000139 DAWAOG010000000
VERSION     DAWAOG010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2917_length_3973_cov_2.586524"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1197 a          770 c          929 g         1077 t
ORIGIN      
        1 tagtacatga tgagctgata aagcttatgg gtacggatac ggcaaggatc gatttcccgt
       61 caaagcctcc gtgcgttatt atgatgtgcg gtctgcaagg tgcaggtaaa actactcata
      121 ccgcaaagct cgcaaagtat ctgaaaaagc agaacagaag accgctttta gtagcctgtg
      181 atatttaccg tcctgccgct atcgatcagt tgaaggtagt aggcgcttcg gttgatgcac
      241 atgtgtttga gatgggacag acaaatcccg tgaagatagc aaaagagtca ataaagtatg
      301 cgaaggacaa cggttttgac gttgtaattc ttgatacggc aggtcgtctg catatagatg
      361 aggacctgat gaacgagctg aaagacataa aggctgaggt tcagccgaat gagatattgc
      421 ttgttgttga ctctatgacc ggtcaggatg ctgtaaacgt tgcaaaatcg ttcaacgata
      481 tgcttgacat tacgggcgtt atccttacaa agctggacgg cgatacacga ggcggtgcgg
      541 ctctgtcagt cctttccgtt acgggcaagc cgatcaagtt tgcaggtgtc ggggagaaga
      601 cagacgacct tgagcagttc caccccaacc gtatggctga cagaatactc ggcatgggcg
      661 atgtgctttc actcatcgaa aaggctaaga taaatgttga tgagaaggag caggaaaagc
      721 tcgcaaagcg tctgcaggaa aacaagttcg atatgaacga cctgtacgcg cagtttgaac
      781 agattgagaa gatgggaagc atctcatctc ttataaagat gatccccggt gtagcgggca
      841 aggtcaagga agaggatata gacgagcgca gaatgtaccg cactaaggct ataatttctt
      901 caatgacaaa aaaagagcgc gaaaagccct caattatcga tgccaagcgt aagcgcagaa
      961 ttgcggcagg cagcggtaca aaggtcgaag atgtaaatca gcttctcaaa cagtttgaag
     1021 gtatgcagaa gatgatgaag cagatgggac ttaagggcgg taaaaagcgt aaatttccga
     1081 aattccccat gggtggcatg ggaggaatga acggcatggg cggcttcccg atgtaagttt
     1141 gctatgtaaa tacaggtaat tcatggtgtt tgacaataca ctttgattat aaaaactaaa
     1201 actttaaatt ttttatgagg tgaacaaaat ggtaaagatc agattaagac gtatgggcgc
     1261 taagaaggct cctttctaca gagttgtggt tgcagattca cgctacccca gagatggccg
     1321 tttcattgaa gagatcggtt actacgatcc tacaaaggaa ccctctgttg taacgatcga
     1381 taaggaaaag gctgataagt ggctcgctaa cggcgctcag cccacagaca cagttaagaa
     1441 gcttctcaag atcgcagctg aaaagtaatc gctgtttctg aaaggaaaat aaaatggaaa
     1501 aattgcttat cgctatggtg gaagaccttg tagatgacaa gtccgccatc cgggtaacag
     1561 ttgatgagcc caaagaggac ggaactgttg tttatcatct gcacgttgct cccgatgata
     1621 tgggacgtgt aataggcaag cagggcagaa tagccaaggc tatccgcacc attatgcgcg
     1681 caggcgcagt aagatacaat cagaaggtta tggttgagat cgactgagcc gcacattaaa
     1741 agatacagaa ccccgatcgt ttaaagatcg gggttttgtt tattaactga atgttaactt
     1801 tactgatttt tgttccgaca gctcattccc gtttaacagt tttcacaaaa aatcgggtgt
     1861 gcctgcaaaa cagttgctaa tttaattgac tttaattaac agatattata taataaactc
     1921 ggagaagtgg atattttggc ggaatatgcc gtatcctcaa aaagaatttt ttgcagaact
     1981 gcgtcatagt ataaatcgga gaaaattatg gaacatttcg gaattgaata ttggatgttt
     2041 acgttctgta tgtattgtat gatagggtgg gtacaggagt cgactataga gtcgctgtat
     2101 cacaggcgcc ctataaaccg aggatttctc aaaggtccgt atatccctat atacggagtg
     2161 ggcggacttc ttcttttgtt tatctgtcat ccgttcagaa ataatgggtt cgaggttttc
     2221 tttgtggctc tgattgcctg cacggcgctt gagtacttta cgggctggct tatggaaacc
     2281 atgttcggta agcagttctg ggattacagt atgttcagga taacctataa aaacagaata
     2341 tcgcttatat cttcgctttt ctggggcgtt atgggattgt tcgtgactta tgttgtttcg
     2401 gacgttacta tgtttgtgct gaatagcctg ccgcatcagt ttatttgtgc tgcgagcgct
     2461 acagtatcac ttataatggc tgttgacttt ttgaatacaa caagaaagca gatagatgtt
     2521 gacaaattaa gaagcacttt cagcatatct aatataagtg ctcatattat ccgttttgac
     2581 actattgcgt cgcgtatgcc aagactcaga gcgagaaatg acgaaaagga cattgataat
     2641 aaagaagagc ctgaatacag cggcgatgag gaaaaaaatg actgataata catttacacc
     2701 tacagatgac gatcgtaagt ttataaatat cgcaagaaag atccttgcca acgaaaaggt
     2761 acagcagatg aagcagtatg ttcagcacgg cgatgtttcc acatatgagc attgccttat
     2821 ggtgtgcctg tatgcttata tgtatgcaaa gaggcttaag ctgaagataa atatcgaggc
     2881 gcttgtaaaa ggggcgcttc tgcacgattt tttcctctat gactggcaca agggcgcatt
     2941 cacaagggac ggtctgcacg gattttcaca tcccgtgacc gccgaacgta atgcaaggaa
     3001 cacgttttct ctgacggtta aagagcgtgg gataataata aatcatatgt ggccgctgac
     3061 attgaccaga cttccgagaa gcaaggaagc gtggctggta tgctgggcgg ataagtattg
     3121 ttcgcttaaa gaaaccttgc ttaaaaagcc gtattgaaat gaatgttaaa gcctgcacga
     3181 acaaaaatcg ttccgtgcag gcttgtgttt tatacaattt atttcacagt aaaatatttc
     3241 tgcgtgtggc tccgtttgga tttcggttct gctaaggcga gtataccgtc atcgaccaga
     3301 gggtctacat aacgttttat acaatatgac acagatgcca ttctgagata ttcggcaatt
     3361 tccttttttg atcttggggt aacgcagaaa tcaaaacgcc aaggatacat ccaccaatag
     3421 cattgttaag cgaggctcca aggaacaatc ctgcaactcc acacaaaata gatacaactg
     3481 ttctaaactt gttcatattc gccctacatt aaatatcctg attcttctgc ttgttacgct
     3541 tcttgttaca caacgaacct aagagcatac cgattgcaga aattattgca ccggctacaa
     3601 tcattcccca atccggtgca ttcagcttgt caaaagcaat gttgtttgcc attttaaatg
     3661 ttccgtattc agccagcata tacatcactc cgaagaaaag ggtgaatgcc cattttcctt
     3721 ttttccaaaa gtcatttttg ccgataacaa tggacacaac gaatgtagtt acaggaagaa
     3781 taatccaaag gaacatcaaa ctataaccca tagcatcact tccgcttgta aagaaccaga
     3841 acacaatcat ggcaatcgcc catacaagca tataagatag gatggtaata attttgtttc
     3901 tgttagcgtt gctttttaca acatttgtac tttcttcgag ataatcataa taggttttca
     3961 ttttttgctc tcc
//
LOCUS       DAWAOG010000140         3959 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2924_length_3959_cov_2.057889, whole genome shotgun sequence.
ACCESSION   DAWAOG010000140 DAWAOG010000000
VERSION     DAWAOG010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2924_length_3959_cov_2.057889"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1032 a          858 c          871 g         1198 t
ORIGIN      
        1 tagtattggt gcttagtata ggcggcaaac ccagagctac gagaacttta cggcgctttt
       61 cagcactgcc gggaaagcct gccataccgc cctccatcat atcagctttg gtaaggaagt
      121 cctctttctt gcagttttct gcagtaacac ccgcttttaa cagcgcatcg cgcaggatat
      181 cggagcttat accttcgact ccgagataac cctcggcaga tggattaagc ttgcgctttt
      241 ctttaccttt tacctgcggc acgaatacgt ttgtgatctt tccgccttta cagcagcctt
      301 ttatatgatt tcttatacgc agtcccgctg tgtcgctgtc ggtaagtatt actatcccgc
      361 aggtcaaagc aagagtacgt attagctctg ttttttcttt gtccttatat atgccgaagc
      421 cgtctgttgt gattattacg gcatctagca gggaagagag ctttatttta tcgtatttac
      481 cttcgactat cactgcctga tttaatttta tcaatagtac gccctcctgc tcttttcggg
      541 agaaagcttt actatcgttt cttcaagcac ggttctgccg tttatggcag aagttttaag
      601 caaatgatcg gcattgaaca gtatatcaag gcagtttctt atatgtgtca tatcaagccg
      661 tgaaaccagg ttcattgctt ttgtcacaac gaatgtacgg ttctttgcgt agccaaagtc
      721 ttcggcaacc agtgatgcag acttgcctgt actttgtgcc gctttagcgc gatagaagtc
      781 aatgtagttt gacgagagtg atgcaagtat cacgataggg tctatcttct gtgcaataag
      841 atcgtcaagt ataagaagtg cctgtttgcg gttacccttg gtaagctctg ccgcaagcga
      901 atatatgctt gtgtcaagct gctttatagt caacttgtct atcatttcgg cggttatctc
      961 gcctgtgccg acataactgc acagcttgtc ggtttcggca gagctgagcg ataagtcaca
     1021 cagagtaagc tctgctatat gctgagcgtc aggtcgggat atcacgcagc cgcgttttgc
     1081 cgcttttttc atgataagtt ccgtgaccct tgcaggagta agatgcgtaa actcgcatac
     1141 tgcagcgttt ttctgctttg aaagagcggt aataagcttt ttgttctttg ctttttttat
     1201 ctgttcggag cttcccgtta tgctgattat gacggtgcac tcatcgggta tgtctttaaa
     1261 cgaggaaagt atcgtttcta tggcgttctc atcaaacttt tccatatcaa ggtcgctgat
     1321 aagcacgacc tttttgtcgg aaaacagcgg catactttct atgctgtctg taagaacgtc
     1381 aacggggaac gtgctgccta gctttgtcag gttgatatcg tttctgtctt tacctatagt
     1441 tgtgtcaaga agtcggtcgg tgtaggtttt tacaagaaat cgctcatcgc cgtaaagata
     1501 gtttatgcgg ctgatatctc cgctttttat ggcagatgat agtttatcct catcatactt
     1561 catattatcg ctcctttcta tctgtttact gtttggacgg tcattccggc ggcggtaaca
     1621 gttattttgc aatccgtgta atacactgat acacagtttt tgctttttga gtatctgttt
     1681 atgaaaaagc atggagcgga tgtagaaaca gatttgcttt tgccccacag aatattagca
     1741 gtattctctt ttatatttct gacatctgag atgtttatct gtgtatcaaa tgctgtgagc
     1801 agtaccgagt tatttttaca gtcaagtgaa aagtatctgc cgacaatacc gttattgtcg
     1861 ttgcaaagtg atacagcata tgaaaaaccg cttgccgtgc cgataatctc atcatcataa
     1921 tcatcaaggc aaagaacagt aaggtctgaa gcggttttat ttctgtcaag tatttcgctt
     1981 cttattatgt tgagctcctt cttgtcattt ccgataagaa cggcagctga aatatcatcg
     2041 gagattacaa cgacagagcc gttttttccg tcactgtgaa gaagtatttc tacattgttc
     2101 atcggcagaa gcggaagcgt tgcatttatc gctgtaaagc acagcgcggc agtcagcaag
     2161 attgccgtta tatgttttct gcgagtcagt atgatcgcaa ttactgcggc cgtgagagta
     2221 acaataagtg tcattattac agtgagtgag tcacagctga aatgcaaaaa aggtatagtg
     2281 gcaaagaacg agaacaccgc acgcattata tcacagcaaa aatgtgctat gacggcaact
     2341 ggtgtgagaa atccacctgt caatgcaaaa ataagcatcg ctgtaaggca caccgtgaaa
     2401 agcgggagca cgataaccga tacgaatatg ccggcaagtg aaaacgtacc gaaatataca
     2461 gcggacagcg gtatgccggc aagtgccgcg catattgaaa cgcacagcgt atcgcaaagc
     2521 tttttattaa gccgtggaaa taaaccggta agctttttgg atacaccggg agcggcagca
     2581 cccgcaccga gtgttgtgac agccgacagt ataagtcctg cgtcagtgca ggatagtgga
     2641 tcaaaaagcg ttataaccag caccgcaaga cctacagagg ttatgcagtc gctctttcgg
     2701 tagaaaaccg caccgatatt cgatatgatg atcattattc cgcttctcat aaccgatggt
     2761 gtaaatccga aaaaggtcat aaagcacagc gtcagcagta ttgttagcaa aaagcgcact
     2821 cgatatgcct ttgctgcgcc cgatgcggat aacactgcta taagtatcgt cataataagc
     2881 gttaagtgca tacctgatac tgctgtcagg tgagcaacgc ccgccgcctt gatattatag
     2941 tacagttcat cagaaagcgt acttttatca cctgcgaaaa tacctttcat aagtgctccg
     3001 ctgtcatcgg aaaactctgc atcgattttg cttatcagat aactacgata gttgtcgatg
     3061 aatgacagag gataactgcc ttttgttacc gtgatatcac ttttcagcgt tgcagacagc
     3121 gtaatgccgt ctgagcggta tctgtcggcg attgcgtatg ctgcagtgtt ataaagcttg
     3181 ctgagctctg catcaaagcg gagcgtgtcg cctttttctg cgtttatatc gtctgagtag
     3241 agcagtatac ctaccttgcc gttttcggtg ttgctttcaa gtgtaaaaat tgcactttct
     3301 gtgcctgttg tcttttttga agttacggtt gcgcttatat catatgtacc gcccaaaagg
     3361 ggttctgccg ctttgtcaag ctgtgaagat actgcaaaat atacggcaat accaattgca
     3421 cagcaaagca gtacagcggt aatatcatat tttttcggta taagtgccac cgtaactgct
     3481 gagagtacaa gtaatattac tccacaaatg agaaaaattg caaattttat gaaaattgag
     3541 gcgaaaatca gtcctatcag atatgctgct ccaacagccg ctaatttccg cctcacgatt
     3601 atttaccttt tatgcaagct caacgctcag ctttttgccg ccaagtagat ggaaatgaag
     3661 gtgacgtaca gtctgtcctc cgtcttcgcc tacattggaa acaagtcgcc agccgttgtc
     3721 aagattgaat gactttgcaa gatgcttagc aaccagcata atgttcttta caacactgac
     3781 attactttca tcaagcttgt ctgcaccctc gatatgttct ttggggatta taagaatatg
     3841 tacgggtgcc atagggttga tatccttgaa agctatgact ttatcgcttt cgtatacctt
     3901 ttccgaagga atttcgcctg aaattatctt acaaaaaatg cagtccatac tttatttcc
//
LOCUS       DAWAOG010000141         3958 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2927_length_3958_cov_2.542147, whole genome shotgun sequence.
ACCESSION   DAWAOG010000141 DAWAOG010000000
VERSION     DAWAOG010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2927_length_3958_cov_2.542147"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1397..1406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2664..2673
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2915..2924
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          950 a         1046 c          828 g         1104 t
ORIGIN      
        1 cgtaccgctc ctggcaaggc tttcatcata atagcataca cagccgtccg caagctccac
       61 cattcgcgca ttgcgctttt tcatacactc cttggtgtac tgtgagccga tatactcaat
      121 atcgtcagca gccataagta taccgtagaa ctcctgcttg tcgctgtagc tccagttctt
      181 tgtctgctcc tcattagaac aggggagtat aagatgaagc tttatatcga gcgctttgcg
      241 ctttttaagg taaagcacca tatgtgcgca cattgtatca aaaccgagcg cgccgccgca
      301 ataaaaatcg gtcacgccac gctttacaag tccgcatatc gtgctgtcga gaagtgtctt
      361 aagaccgtac atatccgcac attctatctt acggtgtccg gtaaagcaga cagatgtcat
      421 tgcaaagccc cctttttgta gcgataaaaa gaaaaataag ccgtatctgc gtctgacaaa
      481 tacggctgat tatgctaaag caagatgtaa atcagtcttc aaccttttct tcgatgaact
      541 taacaatatc accgacagtc ttgatcttct caacctgatc atcggggatc tcgatgtcga
      601 actcttcttc aaagctcata acgaggtcaa caacgtcaag tgagtcagca ccgaggtcat
      661 ctgtgatgct tgcagcttca gtaaccttgt cttcttcaac gtcgagctgg tcaacgatta
      721 tcttctttac cttatcaaat accatttgaa atctcctccg tgttttgtaa aagaaatttt
      781 ctttcgttaa tattatacgg cattacgccg aaataatcaa gtcgtattta caacaaatac
      841 attaaaaaaa tgtctaaatt tgcagggtgt atttctacac tttgcacaac tttcaaaggg
      901 ttgccgaaag ctgttgcaat accgctcaga ataatgaata agcagtaaga tattgtcacc
      961 gccggtaacg caagtcacgc agaccgccga cattctgaac ggcgaacagt cagcggcgac
     1021 tcgccgcacg gcgaatttac tgtgactttc agttactccg agaaaacgcc gattttgcgg
     1081 aacttcttgt aacggttgtc gagaagtgtc gggatatcaa gctttgacag cctttcgacc
     1141 gcgcttataa gatattccga aatgttgtcc gccgtctgag cggggtcggt atgagcacct
     1201 cccaccgact cggaaatgat tttgtcacat acaccgaagt tgtacagatc atctgcggtg
     1261 atcttcatca ggtctgccgc ttccttttct cttgacggat ctttccacag gatagacgca
     1321 aatcctctcg gtgagatcac cgagtataaa gcgtgctcaa gcattgcaag ctcatcacat
     1381 accgcaagcg ccagcgnnnn nnnnnntcac cgagcactac cgatataaca ggcgttttca
     1441 gtgtcataaa ttcggcaatg ttcttagcta tagcctcacc ctgacctctc ttttccgcct
     1501 ctacaccgca gaatgcgccg ggtgtatcaa caaagcatat aacgggtctg tggaacttct
     1561 ccgcctgata tgcaagtctt aatgcctttc tgtaaccctc ggggtgcggc attgaaaaat
     1621 tcgtcttctt gttttcttct aagtttctgc cctttacctg cgctatgacc gtaacaggta
     1681 tgctgttaag cgttgctata cctgacatta tagccgcatc gtcaccgtaa tgacggtcgc
     1741 cgtggaactc ggtaaaatga ttgaatatag cgggtatata gtcattcacc gtcggaaggt
     1801 tcttttctct tattatctca agatgctgtg tagcagtcat atttgacatt attccttacc
     1861 gtcctttcaa ccgtgctgcc agtcagcgcc gattttttta tagttatgca gtcttagtat
     1921 attcgacagc gttttacgca ttgagcgtct gtcggctatc gcatcaacaa aaccgctttc
     1981 aagcaggaat tctgccgtct ggaagtcgtc gggcagcttc tgctttatag tgtcctgaat
     2041 aacacgtctg cccgcaaatc caacaagcac cttaggctcg gcaaggatta tatctccaag
     2101 actcgcaaac gatgccgtaa cgccgcctgt agtagggtct gtaagcaccg tgatataaag
     2161 tccgcccgcg tcgctgtgac gttttaccgc accgctcgtc tttgccatct gcataaggga
     2221 gattataccc tcctgcattc tcgcaccgcc cgatgccgta aacagtataa caggcagctt
     2281 atgctctgtt gcatactcaa acgcccttgt tatcttctcg ccgacaacac tgcccatgct
     2341 ggccatcata aagtttgagt ccataatgcc gatgaccgca cggtagcctc ttatcgtgca
     2401 ttcgcccgta acaacagcgt ccttaagacc ggtgttgtcc tgcataccct ttatcttctt
     2461 ctcgtaatcg ggaaagccga tggggtttaa tgacaccatg ttttcgtcaa acggcttgaa
     2521 gctgtccttg tcaaccgtta tatcaagacg ctccttagcg gaaagtcttg catgatagtt
     2581 gcagacagga caggtctttt tgcgcttttc aaactcgtcc atatagaaca cgttctgaca
     2641 gcgcggacac ttgaacagca gatnnnnnnn nnncttgggt atttcggggg cagaagcctc
     2701 cgcctcgtcc ttctgttcct cgttaaaacg gtccttgacc attttaaaca gatcttctaa
     2761 caattaaagc accgccttta ttgttcaaat cagataacaa tattatctgc cgatagatta
     2821 aggctattga cccatccccc ttgccccttc ccgggaaggg ggagaagata tgggggctat
     2881 gcccctgcga ccctaccgta gattatattt tatcnnnnnn nnnnaatcaa tgttggtgtt
     2941 gataccctca atgagaaatt cggagagtgc gaccttcatc ttctggatag cctctttcct
     3001 gtcctcaccc tttacaagca cctttgctat catactgtca tagtaaggcg gtatctcgta
     3061 gccctgatat accgccgtat cgatacgcac gccgaaaccg ccgggcatat ggatagactt
     3121 tatctttccg ggacagggac ggaagttgtt ctttggctct tccgcattta tacggcactc
     3181 gataacatga cccgtgagct ttatatcctc ctgcttatac tgcaagggga gccctgcggc
     3241 aatgtttatc tgcgccttta caaggtcaac gcctgtcacc atttctgtta ccgggtgctc
     3301 gacctgtatt ctcgcgttca tctccataaa gtagaagttc agatccttgt caacaaggaa
     3361 ttctaccgta cccgcgttcg catagccgca agccttagcc gctctgactg ccgcttcacc
     3421 cattctccta cggagctcct ccgtcataat aggactgggg ctttcttcaa gcaccttctg
     3481 attacgacgc tgtaaagagc agtcgcgctc gccgagatat attacatttc cgtgctcgtc
     3541 cgcaaggagc tgtatctcaa cgtgtcgggg atttacaata aacttctcga tatatatatc
     3601 gtcgtttgca aagaactgtt tagcctcctg ctttgcggcg gtcattgccg cttcaagctc
     3661 ctcaggcttt tcaaccagtc ttatgccgcg tccgccgccg cctgcgctcg cctttaccat
     3721 tataggataa ccgatctttg cggcgatctc ctgcgcctct tcaagcgttt caacaacacc
     3781 gtccgagccg gggactacag gaacgtcatt ttctatcatc gtcttctttg cgttagcctt
     3841 gtcgcccata gcgtccatag cgtcagcgcc cggacctata aacttgattc cgcacttgtc
     3901 gcaaagacgt acaaagtggc tgttttcgga aagaaaaccg aaaccggggt gtattgcc
//
LOCUS       DAWAOG010000142         3934 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2948_length_3934_cov_2.758443, whole genome shotgun sequence.
ACCESSION   DAWAOG010000142 DAWAOG010000000
VERSION     DAWAOG010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2948_length_3934_cov_2.758443"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    866..875
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1674..1683
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1039 a          898 c          793 g         1184 t
ORIGIN      
        1 attgctttca gcggtgttgg ctgaatattt attgttttga catttttata atatacggca
       61 ttgcaattat aacgaaattt aataagctga ggaattgtgt aacagccccg gaatattcgt
      121 tttatcggtt gctgttatct gagctcgtgc tctcgccatc ggacgaatta tccgtattgt
      181 tttcggattt atcgccggaa ctgccgtcaa cttcaggctt ttctaccgcc gcgacatcat
      241 tcatatgcgt tattgtcata aggtagtcga ccttgccctg cgtgccgtcc tttgtagcat
      301 aacctatctg ctccattgag gtgcaatatc cgtccctatc aaggttgtac acaacactga
      361 agctgtccag gtcgccgact agtccgagct gagcagacat tgagtcgttc agctttgata
      421 catcgtactg cattgtgacg gttttgccgt cggctgtttc tttgacctcc gagcctgtaa
      481 cacagtcagg ctttataaat atcattccct catcagtcat tgacatttta ttcgttcgat
      541 tgtagctgac atagttctga tctccgctta caagcgtatg ccattcaccg tcggaatagt
      601 aattatattc cgagccgtta tgatactcat aatattttgt attgccgtca gttccgaaat
      661 agctgtatga aagcgtatca ttctgatcat agcagaatgt cagctcctgc gtgactgtac
      721 ctgtcccctt atcggtaacg gttagctcag cggaataaag cgaagcgtaa agctttctgg
      781 cattttccac atcactgtag ccgtctatat gcgaataatc cgcactgcaa gccgttatgg
      841 aggttacagc cgtcatcgcg gcgatnnnnn nnnnnttttc ttgttttttt caatagtatg
      901 ctcctttgtg attttaacaa tttgcaacgt gaaataaaaa tacagcgggt gacctgcata
      961 tagcaaaatc cccgctgttt tttataaatg taaattctat cgtctacact tttctacacg
     1021 tctacacatc gggatcaacc cgactacact acttgaccgg gagtgtaaat tattcagcaa
     1081 agccgctttc aacgagctta acaagaccgt caaccgcgag ctgttcgtca gatccgtcag
     1141 caattattct gatcgttgta ccgccgacaa taccgagtga aaggataccg agaaggctct
     1201 ttgcgtttac tcttctttct tccttttcaa cccagataga tgacttgtac tcattagcct
     1261 tctggatgaa gaatgttgcg ggtcttgcgt gtaaacctac ctgattctta acgcttactt
     1321 ctttaacaaa catgattggt tacctccgta aaatcgtatt atctttccgt cgagccgtcc
     1381 gcattgagat gatatgcgtt atttctgctc tgtacctata gtatatccaa atgttctttg
     1441 ttcgtcaaca aagttttttc aatttgtcaa tttttctgcg attatttttc atatttgttc
     1501 aatagtcctc aatcaccgca gcgcaatttg tgtgttatgc acaaaaaact ttcaacaaag
     1561 tattcaacat acacattagt tttcaaccgt tttgcttatt tcggctgttt taccctttaa
     1621 aatgcttact cttacaaaaa tggctgtcgc tgaaaacggg gcttcttcaa ctgnnnnnnn
     1681 nnnctgccgt aattgcctgc ttttaaaccg aaatcacagc ctgcacttga aatcctcata
     1741 cgaaaactct ctgattttac gcataccgag cgtggtgtta taatatattg acggcaaagc
     1801 cataccattg aatgtattgt tctttaccat cgagtatatg gacatatcgc acagcacaag
     1861 gcggtcgcca ggccccagcg gcttgtcgaa agaataatcg ccgatcacat cgcctgcaag
     1921 acaggtgttt ccgccaaggc gcacggtgac agccttttca cccttttcgc ctgcgatatt
     1981 tccgtttggc tgaatgataa cggggcggta cggcatttca agtacatcgg gcatatggca
     2041 ggcggcagac gcgtcaagta tggctatgtc gtcctcgccg ttaattctgc cgtctataac
     2101 gtcaagcacc gttgttacaa ggaagcccgc gttaagcgca accgcttctc ccggttcaag
     2161 atagacctca agaccatatt cgtttttgaa atggttgatc acctcaacaa gggtgtcaat
     2221 atcataatcg tcccttgtta tatggtgtcc gccgccgaaa tttataacgc tcatacgatc
     2281 aagatactta ccgaactgaa cctcgacctc ctttgcggta cgctttagat catcgctgtt
     2341 ctgctcgcaa agcgtgtgaa aatgcaatcc gtcaatttca ttgagtattt cctctccgta
     2401 gcgctttatg tcatcttcaa acgaagcaag cgtctgaccg agccttgatc ccggagcaca
     2461 cgggtcatat atctcgtgcc cctcctgagt cgaaaacctc ggatttacac ggataaggca
     2521 gtgcttatcc ttcactgcgt cacggtgcat ggcaaactga cggatagagt tgaatacaaa
     2581 atgtccggcg tactttgtaa gctcgtttat ttccttgtct gtataggcag gagaaaaaac
     2641 gtgtgtttcg ttgccgaaat attcatagcc gagccttgcc tcatacagtc cgcttgcagt
     2701 agtaccgctg agatatccgc ttataagagg ataggtgctg tacattgaaa acgccttctg
     2761 cgcaagcagt atcttacagc cagtcatatc ctgaacgctt ttcagtatct cacagttgcg
     2821 tttaagatag cattcttctg taacatagca aggggtatct gtcttattta tatcaaaagt
     2881 aggtgtgttc atatattgct ccagtctatt tcgtcatcgg cgagataatc atcaacgtca
     2941 aagcggcgtt ttttgaagta gtaatgcttg tcctctattg actgcctgac gtccaaggtt
     3001 tccctcggtg ttattttagc ataatacgtc ggaaaaagca actcaaacga acttgacaac
     3061 acacaaaaat atggtataac aacctcagcc tgaaatcgtc agaagacgat ttcgccgcat
     3121 aatatgcggc tttgctacgc aatggctgag aacgttgaaa cacgattttg ttttgcccga
     3181 taggcaaaat ttgcggcatt tgccgctatg tgccttgacg gcacattaaa atcgtgttat
     3241 aatataaata aagagggaaa cgacagacgg tcgctcccta aggttggtta aaataaccgc
     3301 tcacagtttg cgacaggggg cggttatttc tttttgttaa aagataaaat gctgagtata
     3361 actgcggcaa atcctgttag gaaaatacca atctgaatta aattatccaa tgttatgtac
     3421 acagcttcac ctcctttcaa agcgctttcg cactttcggg agatgaattg accgcctgac
     3481 gtccaaggtt tccctcggtg ttattttagc ataatacgtc ggaaaaagca actcaaacga
     3541 acttgacaac acacaaaaat atggtataac aacctcagcc tgaaatcgtc agaagacgat
     3601 ttcgccgcat aatatgcggc tttgctacgc aatggctgag aacgttgaaa cacgattttg
     3661 ttttgcccga taggcaaaat ttgcggcatt tgccgctatg tgccttgacg gcacattaaa
     3721 atcgtgttat aatataaata aagagggaaa cgacagacga tcgctcccta gattaagtta
     3781 tagaataacc gcctcaggtt agtagcatgg gcggttattt ctttttataa aaagaaatga
     3841 ttgtgaatat cataactgtg aaagcagtaa gaaatattcc tatctgtatc agatcactta
     3901 gtgttatgta cacagcttca cctcctttca aagc
//
LOCUS       DAWAOG010000143         3933 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2950_length_3933_cov_2.347344, whole genome shotgun sequence.
ACCESSION   DAWAOG010000143 DAWAOG010000000
VERSION     DAWAOG010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2950_length_3933_cov_2.347344"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1086 a         1028 c          762 g         1057 t
ORIGIN      
        1 attcaaggtc ctcaagatcc ataagcagtt cgttcatcca caatgtagca cgcttgccga
       61 cggtagtcag ctgtgcaggc tgtaaatgtg tatatgcaag acagggcata tctttatact
      121 tatcagcaaa agaagcaaag tttgcaagca cattcacaag ctttctgtgc actatcttca
      181 tagcatcacg cattacgata acgtctgtgt tatcacctac ataacagctt gtagcgccaa
      241 ggtgaataat accctctgcc tttggacaac actttccgta tgtgtgaacg tgtgccataa
      301 catcgtggcg gagcttcttc tcctcctctg cggcatattc aaaatcgata tcatcgatat
      361 gtgccgcaag ctcgtctacc ttttcctgtg tcacctgctc aagcccgagc ttcatctcgg
      421 ctcttgcaag agctacccac agtctgcgga atgtcgaaaa cttcttctga gccgagaacg
      481 tgaactgcat ctcctcactc gcgtatctcg tgcagaaagg gctttcgtag gtttcgtagt
      541 ttgtcattat aagtaaccgt ccttttcatc ggatttttaa ctttccttgt atcggctgtc
      601 atataaactg attaccgcgc ataccgacac attatatatt gtaccacagt ttgtcaccgt
      661 tcgcaatagt aaacatcact ttttctcaaa actcccgttt aaatatgcaa cgctcccccg
      721 ttttatgact gtaaattgta cggtcgcatc gaccacaacg cccgcattta taatgccgtt
      781 cggtcacatc gaccgcaccc cgccgatcct cataataaag caaaacccca taactcgcaa
      841 aaatttttgt ttttttcgca atttttttaa aaaaactatt gacaaacgtc cgaaaaccgt
      901 gtataatatc tactgttacc tgggtgtggc gcagattggt agcgcgctac cttggggtgg
      961 tagaggccgc aggttcaaat cctgtcactc agaccagtta aaaaccgttc gattatagta
     1021 atcggacggt ttttctgtta tattcacaac actaacaata aacatagcct cccgcacaac
     1081 accatgcggg aggctctcta tttcttttat tctctaccct taaactccgt aatatccttt
     1141 attacctcac ctgcgataat aagccctgcc acagacggca caaaggcggt acttcccggc
     1201 acctgtcttt ttcccggcac cttatccgcc ttagaaagac aaccgttttc accgttcgga
     1261 tcttcgtcag caattggcgg tatcggcttt tcctttgagt ataccacctt gagcttcttt
     1321 attcccctgc gtttaagctc atagcgcatt acctttgcca gaggacacac agaggtctta
     1381 tatatatccg taacctcaaa cgctgtcggg tcgaccttgt ttcccgctcc catagagctt
     1441 ataataggcg ttcccgcctt ttttgcgttt tcaataagcg cgatctttcc tgtgaccgta
     1501 tctatagcgt caaccacata gtcatactga gtgaagtcaa actgatccgc cgtttcaggc
     1561 atatagaagg tcttgaaagc cgtcaccttg cagtcgggat ttatttcctc aattcgctcc
     1621 ttggcaacat cgaccttgta cttaccgacc gtctttgttg tagctattat ctgacggttt
     1681 atgtttgtaa gacacacctt atcatcatcg attatattga tcgctccgac gcccgagcgc
     1741 gcaagcgcct caactgtata tccgcccacg ccgaaattgt caagtatcgc ctttttaagc
     1801 tcagatgctt ccgttatgct tattgccata agtcgctcct ttaaatctac cgtttctatc
     1861 tgttttgtgt acaatactgc tattatacat cgtaatcagc gcgctgtcaa tgtcacctct
     1921 gctgtttttt agttatttta cgcgatgcat ttttcctttt caacagatcc gctgccgact
     1981 gcaaaaataa tatatcctct tccggcagtc ccgttatatc cagtgcctgc cttttttcct
     2041 ccagcccgaa aagatagtcg gtcgtgaccc cgaaaacctt ggcgatcttt ataatagttt
     2101 cgggtgaggg atttcgttcg gacagttcat agttgctgac agccgccttt gatacccata
     2161 ttctgcccgc tagctgttcc tgcgtcattc ccgcgtcaag ccttagctgc ttcactcttt
     2221 cacccctccg ttacacaaat acacattccg aatacccaaa atacacgata gctttattaa
     2281 aagtgtagca tatatccgtt ttcgggctgt tgatttttgt aacacatacc gttgactatc
     2341 cgtacacgct gtgttaaaat ataatatgta atatcccgtc aaagtatgtc ggacaatatc
     2401 ggaaacagga gagaaataaa tgaaaacgcc aataagtaaa gctgtcgcag ttattctgac
     2461 tgcggggctg acggcaacac ttgccgcttg ctcatcgcag tcatctcaac gcaccgcgga
     2521 cagctcatat gctgcagccg ttcagacgac cgccgtgcct gaaaccgttt cgacccccat
     2581 cgacacatca gccgcgcccg aagcaacagt cgaaaccaag ccttacacag catatgtcga
     2641 agcaagtact tcagcgaaaa ccgaaaccgc tgtcacaaca acagcatcaa ctacagtatc
     2701 gaccacagtg tcaacccctg cgacaacagc agagccgaaa tggaatgaaa caaaaaaatc
     2761 cgcagaaatg tacctcaata catcggtcta cagccgctca aaggcttatc tcggtgctcc
     2821 gaccgccgat ttatatatga taaacgataa ggtaaaggta gtagctgtaa ccgacaccgg
     2881 ttattataag ctgtcagacg gcaattttat tcattgcgac tacctcagca atgaaaaagc
     2941 agtgatacag acaacgacag tagcaacaac tgccgtcact gtcacagcag ttacttcaac
     3001 tacacaagcc gcacaaacca cacccttgcc ggaaacgaca gtccccaaaa gcgcagcatc
     3061 agtatcaccc aatacaagcg agccgaccga aaagtacgac agctacacat catcaggata
     3121 tcagccactc gatgatataa tattcccaat tcttgatgaa atcatcactc gggatatgac
     3181 agaagacgat aaatgtctgg atgtttatga ttatctgcta cagtttgact atgtcgagcg
     3241 taccttgtta attcctaaaa gtcaaaagaa gtattccgaa cagctttacg ctataagctt
     3301 gtttgaaaag aagtacggag tctgctatga cttttcggcg gcattcaaat ttatgacaag
     3361 agccgtaggt gttgacacgt tgatgtatta cggacagcat tcaagctatc actctccgtc
     3421 cggatatata ccgcacacat gggatgtcat tgaacgtggt ggcataccgt acatctacga
     3481 catagcaatg gaaaggataa ctatggacgc aggcagagaa gacagctatc attgcagata
     3541 cggcataaca tatgagggct tccccggata ttattacata cccgacacgt tcacaagata
     3601 aaaaagaaag gatatattat gaacaaaaaa ccatcagtaa taacagcagt tctgctttcg
     3661 gtgtgcacag tgctcataac agcgggattg tcagcgtgca acaaaaactc cggcaacagt
     3721 gaaggctcag ccggcagcga aagctcagtc agctcagagg ttcagagaga agcactcaca
     3781 gaagtctcgg caaaacaata tacagcggaa attatgccca tagacgacaa gttttacgct
     3841 atctataacc gttgcagcct gcctgtcgac agctccgtat ccgtgaccga tgccaacggc
     3901 tttgcctact cacctgtcga gagcgtatac aac
//
LOCUS       DAWAOG010000144         3926 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2959_length_3926_cov_2.357789, whole genome shotgun sequence.
ACCESSION   DAWAOG010000144 DAWAOG010000000
VERSION     DAWAOG010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2959_length_3926_cov_2.357789"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    864..873
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3048..3147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1189 a          755 c          891 g          981 t
ORIGIN      
        1 gtgtatgaat ctgacattaa gagcgacagg ttggcaggac tgacatttgt cattacaggt
       61 actctccccg atatgacacg agatgaggct aaaacgctta tagagcagaa cggcggcaag
      121 tgcagcggtt ctgtttcaaa gaaaacaagc tatgttcttg caggcgaaga agcaggaagc
      181 aagctcacaa aagcacagca gctcggcgtt gcggtcataa gtcagcaaca gcttattgaa
      241 atgataggcg aataatttgt aaaaaactat tgacaaatgt gtttttgtaa gatataatat
      301 acttgttaat aggaaaagac gttgatcagg aaaaaagccg tatttttatt cgcagagagt
      361 tgtcgtttgg tgcaaggcaa tattaaaggt cggaaatagc tcgcctgaga gtcgcatggc
      421 tgaacattgc agtaagtcat gacgggatct cccgttacag agaagcacat tgatagatgt
      481 ggcataagag gacatagcat aaggctgtgt caagaagagt ggtaccacga ggatttaccc
      541 cgtctcttta cagaggcggg ggtttgtgta tttacattgc tttttaatgc ctgcatcgga
      601 aaaaccaaat agggaaagga catcatttat gaaaggtaca atgaacacaa acaaatactc
      661 aagaggatat tttatgcctc ctacgcagtg tcttgaatgg acaaagaagg agtatatcga
      721 caaagctccc atatggtgca gtgttgacct gcgtgacggt aatcaggcgc ttatcgttcc
      781 tatgaacctt gaagaaaagc tcgaattttt caaggtgctt gtagagatcg gcttcaagga
      841 gatcgagatc ggtttccctg cagnnnnnnn nnngccgcac actgatcgaa caggatctta
      901 tccccgacga tgtaacgatc caggtactta cacaatcaag agaacacatc ataaagaaaa
      961 catttgaagc acttgcaggt gcaaagcacg cagtagttca tctgtacaat tccacctctg
     1021 ttgcacaaag agaacaggta ttcagaaaac ccaagaatga aatcatagat attgccgttg
     1081 agggcgcaaa gctgttaaag gaatacagag aaaagacacc cggcgacttc aagtttgaat
     1141 attcacccga gagctttacc ggaacagagc ctgaatttgc acttgagata tgcaacgcag
     1201 tgcttgatgt atggcagccc actcccgatg ataaggttat aatcaatctg cccgttacgg
     1261 tagaaatgtc acttccgcac gtttacgcaa gtcaggtaga gtatatgtgc aagaatatgg
     1321 tgaacagaga gaacgttatc gtttcacttc atccccataa cgacagaggc tgtgctgttg
     1381 cagacagtga gcttggcttg cttgcgggcg cagacagaat agagggtact ctgttcggca
     1441 acggtgaacg tacaggtaat gttgatatag taacgcttgc actgaatatg tactcgcacg
     1501 gcgttgaccc gaagcttgat ttctcggatc tgccgtcaat aacggaagtt tacgagaaga
     1561 tgacctgtat gaaggtttac gacagacagc cctacagcgg ctcgctcgta tttgcggcat
     1621 tctccggctc acatcaggac gctatcgcaa agggtatgaa gtggcgcgaa gaaaaagatc
     1681 ccgagcactg gacagttcct tatctgccta ttgatcctat ggatatcggc agacagtatg
     1741 acggtgatgt tatccgtatc aacagccaga gcggtaaggg cggtatcggt tatcttttac
     1801 agcagaagta cggtgtagat ctgcctaaga agttccgtga aaaagtcggt tatacggtaa
     1861 aatccgtttc cgatcatcag cacaaggagc ttactcccga cgaagtgtat gaagtattcc
     1921 gcagtaattt tgtgaataaa gaaacagcac ttaaaattac cgaggcgcac tacaagcagg
     1981 aaaacggcgg aattaccgca acagtaacag tagactacaa gggtgataag gctgtataca
     2041 ccggaagcgg taacggcaga cttgacgcag tgagcgacgc tataaagaac ggtttgggac
     2101 tgagctacag cttagtaaca tataatgaac acgccataga agtaggctct aagtcaaagg
     2161 ccgccgctta tgttgagata tgcgacaaga atggtaattc gttctggggc gcaggtatcc
     2221 atacagatat catcaacagt tcggtaagcg cacttgtttc tgctatcaat aacagcgata
     2281 tggttaagta aatatatttc ggccgccttt taaggcggct gaatttttaa gttgattttt
     2341 cattttaaat attatataat tatcccataa aataaaaaag cggagagata atatgattga
     2401 ttttactgtt ttggataaag cgatcagaag ctttatggaa caatcccgga tttccttttc
     2461 atattcgttt gccggtgagc ataatgaaaa acgattatca agggctcaaa aaatttttgc
     2521 aaacaacgta gatgcaagtc aggtatattt acagattgat gcaacgataa gtgatagcgg
     2581 caggagcggc tgtgtttata catataatga agtatttata aaagacacag gtgaaagatg
     2641 cacaatatct tatcacgaaa tcaaaggtct tacgtttata ggaaaatata attttatcgt
     2701 atcataccga gatggctctg ctgttaaggt atttgcacct tgcagtcctg tggaagtagt
     2761 aagtgtctta tgggcttttg cttcttatta tgcgttatcg gagcatcctg aggatccgac
     2821 agtaacgaca tcatatgcag tgtcgccgac tttaacctgc gtatttgaca gtgtgatccc
     2881 ctcccctgct ttgcagacac cgcaaagtaa aaacgagaat accgatacat caaagtcctt
     2941 tcaatcggtg gatagtgcaa caaataagca ttcaatacag tgtgaacaga acgttgaacc
     3001 tattcaggag atatccgcgt gcaatgaaac ggtagataag atgatatnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnngta caaattgcgg agcggtttta caggacggat
     3181 acgcattctg tatcaagtgc ggcaatcctg taaatgcaac acaaagcatt ccgacaagca
     3241 gacaggataa cgctatatcc cattctgtaa ataattctgt gccgatacag aataactcta
     3301 tagaaaaatc gataccacag cagaacagca caagcccggc aagcttctgt acaaattgcg
     3361 gagcggtttt acaggacgga tacgcattct gtatcaagtg cggaaaacct gtttgtgcag
     3421 tacagaacac aaccgaacag gcgcaaggta tgccattcaa tccgcctgca gcgactcaac
     3481 ctgtactcga tcttcacccg cgatgcagct ttgagtatat acctataaag acagacattc
     3541 ctgcgggaac agcaagttat tccggcaata cgccgaaaat gatagatttt tgttgtaccg
     3601 gtgacgatat tgcctatatc agagatcggc tttcgtcttt ttcgttaagc acagcgtata
     3661 cagatgaatt gccgccaaaa aagctgatga gcgcacttag atatatggct ccgggattac
     3721 ctgcgatgaa agtagtcgga tttgtggata caacgctatt cggttcttgc aaagaaggta
     3781 tacttatctc tcccgatttc tttttcctac gaaaaggtga cgaaaagttt tctatcgact
     3841 ttaaaatgat gagaaacgtg accatagata aaagttctga tactgtaacg gtagaaatgc
     3901 ttaatggtat ttattatcct ctgtgt
//
LOCUS       DAWAOG010000145         3892 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2986_length_3892_cov_2.776127, whole genome shotgun sequence.
ACCESSION   DAWAOG010000145 DAWAOG010000000
VERSION     DAWAOG010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2986_length_3892_cov_2.776127"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          924 a          886 c          718 g         1364 t
ORIGIN      
        1 tgacagcaga taagcatgca cagaaaactc ttcggaggat aagccgaatg ctgtattagg
       61 caatacaaag tagcttttgc tgttctgcat tgggttatcc tcctttatta aattttactg
      121 ttcgttttct tctgcctgtt cgtttataat tgatttaatc tttttaagct catcgttata
      181 atctttttca ttgggataac gctcatagac attcacatat cccatacttc tgagcttgct
      241 tgctatttcc tttgccgcag tgcgtcctgc actatcatta tccaggcatc gtgctttatc
      301 tccgtacctg tggttgttac gatagtcgga aaatcaaagc cgtattcctc tgcaaacacg
      361 tccgtatcaa ttatgtacgc aagctgtacc gccgagcagt tgtacgtata atttggctta
      421 tcttttacta ccttgcgttt ttctgcgtca taaacgtcat agatatacac atcttgtatg
      481 cactcgccta catcgggaag tatctccgct ctgacctcag tatcctcatc attcagatac
      541 agtgttatac ccgattcgta tgcttttcct atcgtgcttg tgccacggct gtgtattgtt
      601 tcaaagtcat tcagcgttgt aaggataccg tttgtatttt cctttaaatc atcggcttcg
      661 accgggagat tgaaaaagcc ttgaaagtac acgaacaatg cacgttgagc tgtactgtct
      721 gacggatacc tttgagtatc tccgccattg ctttctcgct gatatattca gctttctttg
      781 tctgctcctc agcaggcttc ttagtttctt ctgacatttt tctgtcctcc tttggtattt
      841 ttatttatag gtgcgtcacg gtgtcgcact tatatctcca tatcatcgtt ttcacgatta
      901 agttcgtaat cctcgtcagc ttttgcaagt gcaggaacga tttcggtttc aataagcatt
      961 ttgacttcct gcttatttaa aaatttgcca aaaccttctt ttatacgctc ggtactacct
     1021 ctgactactg ttctgccttc tttggtatca tatactctgt aatcgtctgt atggtattta
     1081 ctgaatttat aacagtgttc tacgagcttg ttgtatattt tttcctgctg aattcgggcg
     1141 ataaccgcat ttgtaatatc attccattca aatgttttgt cgaatgaaaa taccggtttg
     1201 ctttcatttt cacgttttac agctatgttt attaccggct ttccgtcacg ttcctcaagg
     1261 ttccaaatgc aatcggtgta ttcgccctcg tatgaataca tagtaccttt ttctttcaag
     1321 aagaagtcac ggacaacttt taccggtgtt tcttcgtact gaatttcatc atctatactg
     1381 ccatatccta taagctcatc ccacgcaaac gcagatttga taaaagaggg aagatcattg
     1441 tgtaactcgt tttcccattc attggtcaga gcaatttttg taagagcctg cattgttgta
     1501 tatgttgcat cgacgaaatc ggggttagtc gggcattttt ttgtttctat caaatcacgc
     1561 aatgcggaat gctgcatagg atatttcacc ttgagtgttt ttcccggtcg atacaacaag
     1621 attattctta aatatttttg tgttatcctt cagtaaaatg ttatcaacat attttgaaga
     1681 aacagcttca gcttgatatg tatctgttgc tgtcggtgaa gctctcagcg tttgcaatct
     1741 ataatcaacc cagtcacggt tatcattaaa gcgataaaaa gccattccct catcgttgta
     1801 tccgactata tgcaaaccgt tgtactgcac tccctgatag atcttaccgg aagctatcag
     1861 tgtattgatg atgttcatat caattcctct gcccttgtta agataagcaa tgatattact
     1921 gtaatttcgt ctgccgtccg ggaaatgagc ctgttccggc agaacaaacc gtacaggttt
     1981 tgatacagat acattttccg ttaccgcctt ccttatcggc gtatatgccg tttgctccct
     2041 atctccgaca agatgtaata ctgcatctct gaaattcata ccccgtatct ttacaaggta
     2101 atcaatggca ttgtttccgc cgacctgtcg gctgttccag taccatctgc gtcggttgcc
     2161 gtttacaaca aggctgttat gctcaataca cttgaattcc ctttccccat aagtgctttt
     2221 aaaggaaaag ccctcctcgc tttcgagaag ggctatcata tcggtatttt ttgctcgttc
     2281 aattatatct tcttcaagat gtgccatact gccacctccg ttatctcttt atgtttgaga
     2341 tatacattct gtagttttca aattccgtgt cgcacgtctg caaaaaccac tgcggcaaat
     2401 taaacttgta cggcagaaag ccgttcatcg taattcttgc cgaagttgca agatttgaac
     2461 aaagatacct gtactgttca ccacacatag acgggtctcc gaaattgaac gctattctgt
     2521 aatctctgcc tattagccgc tttgtatcat tcttgtcagt ttcgagcagt gacataagct
     2581 gtattatgtc ggtttcgttg aaaccgctca caatgtatct gtagtgacag gacagccagt
     2641 tgtcaatctg ctctgtgtct gtgccgtaat cattgattac aacattgtag ataccccagt
     2701 tctccggcac tctgccgaag aaatagaagt cgatgttacg atatgtgaaa tacgttccct
     2761 ttgacaccgt ttcttccttg tatgtttcct ggagtatgtt cagatcgccg tctgtcagct
     2821 cgttcttgct gaagaacgct acctttgctc ctcttgctgt gaaatactct gcaagctgta
     2881 ttgcgtatgt tgtagtaccg acacgacgca ttgctccgaa taaaccgatt gataaatgca
     2941 ggtcgcagtt gttgtaattc ggtattgcat tgttattgcc tttctgtacc gggatatatg
     3001 taacgtcaaa tcttctctga cgttcttctc tgagtcctat tctgcggtcg gcagttgact
     3061 ttatcagtgc ttcacgcagg tctttctgca ttttgttgcg gcttatctgc ttcaacgttt
     3121 tgggcgtttc gcctatgata tttgcaagtc cctgtcttgc tatttccgac agcagcatat
     3181 caccgggata taaaccctcg gcgtatatga ttatcggcac gtccgttgcc atcacaagag
     3241 cctttaagct cgtaaggaat tcatcaccct tttcctcgat aaattcaagg tcgattatga
     3301 tatgagtgat gtactcataa tatttcagct cctccttgat gaaatacgaa agattgttca
     3361 ctgttgttat cttctgattc atctcaagct ccttgcgtat ctcgctgagc attgtctgac
     3421 gtttcttttc taccattaaa agcgggaata aaaacatttt tatacctccg gttcttcttc
     3481 ttcaaatttg ttgtaggatt tagcttcgat cttcgcctta tcagctatca tcttaaacat
     3541 tttctcatta ccgagtaatt tatcagtctg ccaattgttt acatagctga aagtgccttt
     3601 tctgcatact atcagcggta cggttgtatt tcctgttacg ctcactgcat tgacagtcag
     3661 tgctgtttca cagctgacag gattgttcat tgctattcgt ccgtacagcg tcatacagtc
     3721 cgtcatattc agattttcgc aatcctgtct tgtttctccg gacagcgatg ttgttttgat
     3781 tccgaaattt acataacaat tacctatgca aatgcggttt tcgctacaaa acaactcatc
     3841 atacctgtgg ttgttacgat agtcggaaaa tcaaagccgt attcctctgc aa
//
LOCUS       DAWAOG010000146         3887 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2990_length_3887_cov_2.678497, whole genome shotgun sequence.
ACCESSION   DAWAOG010000146 DAWAOG010000000
VERSION     DAWAOG010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2990_length_3887_cov_2.678497"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    451..460
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          982 a          981 c          723 g         1191 t
ORIGIN      
        1 cgcttgccag tatatcctta aacgtatact gtccgagttt gatctcactg taaataccgc
       61 cgcctatagt cacctttgcg tccgttgcag tgtctgatat agtaaattta ttagcgccga
      121 agttttcact tacgccgtca ctgatattta aagtaccgcc aaatacacta ataccgttaa
      181 tgcaaacgga ggtcgagcct tcagggtatg taagagaacc gctatataaa cctacaaagc
      241 aattaaaagc gcagttctca acagtaattt ctgcaccctt aatattgatc ttccgtgtat
      301 aagcgttgat ataattgtcg tcgatcgcgg taatagtaaa ctgaccgctt gacaatgtaa
      361 tatcgttctc aagtccaccg gttgtcacaa gagtgaaaac acagcccttg ttttcagcct
      421 tatccgcata ccgtaagccc tcggttatgt nnnnnnnnnn ttatgtcgtc gaaatacttt
      481 acctttccgt cggcagtagt tacctttgcg taaattttcg caccgcactc actgcacacc
      541 ttgttggcgc tgtcttcatc tacctgaccc tcgccgtcat gcagacactt atatccgcaa
      601 gcgcagttgc cgtgatcatc gtatgtatgc ttcgggtgag ccttaacaaa tatattatcg
      661 cctgctttgt atatttgttg tacgcttgca ttcagtattt ctccgctttc acttgcaagc
      721 gccttaccgc tacccagtat ttcaattaca cttcttccct gaacataaat gctcttgaat
      781 actccggcat tgatcaaagc gtcgccgtct tcaaatgttg caaaagtatc gaattcaata
      841 tctccgttta cagttaccat tccataggaa taaacgccgg tggcgtttat aactgcacgg
      901 ctgcttccgc tgttttcaaa ggttacattt ccgtaaaccg ctactgcgct tatttcctcg
      961 gtaccgaccg aaacattcag cgtatgaccg ttcatgtcga tcgttttatt gccgtttatt
     1021 attatgccct taacaataca atctacatcg ccggtcagct ttaatacacc gtcagcctca
     1081 ttttccgcaa tatttttaaa ctgtgttgcg ctttcaataa atgtataagt ttcagagctt
     1141 tgtgttgtct cggtaaatat agcaagcaca caaacgccgc acggacactt gcccgtttcc
     1201 acgttgtatc tatgctcatg cgctacaacc atgacatttg taagtccgtc gccgatcgtc
     1261 ttcgcactgc cgtcaactat actttcgggg agatgtgtga cactgtttaa ggttgcaaat
     1321 gcatagccct ttgcaagcag atcgtttaca ggtgtgttat cagtcaaagg tccgcaaatt
     1381 atatcgtagt atccgccgta taaagctata ccgcttgtgc cgttttttat tgttaagtat
     1441 gtacaactgc cgccgtagaa ctccacacat tcagccgcat cggcagttac agcgaacaag
     1501 gaaacggctg aactgctgtc atccgcagta ccgtttataa gcgtaccgcc cttattcacg
     1561 gccatttcca ggcctatact gccgttttca ccactgtttt taagatacag cacgcccccg
     1621 tcgtttacgt caattcctct gatttccggt gatataaaat gtccgttcac atcaacagtt
     1681 aacgttgcat tgccgatcac tatgttctgc gaaagctcac agtccttata caatgtcagc
     1741 gtacaaccgt tgttttcctc cgataatgcc gcagtaaaag cgttgtctat attatcaaaa
     1801 tacttcgctg ttcccacgcc cgaaacgccc gcagtaaact gatatttgca gatagagcat
     1861 acacccgttt tgctgtcaac atcgctgtgg gggcatacat atccgcagac gcacttgccc
     1921 gttgctttgt cgtagctgtg ttcgtaatga tctacaacag taaacgaatc gtgtatagtc
     1981 tttgatgtaa acttttcata aatatcaaca tactcaccgc tgttgtcata ttgcagtgcc
     2041 ttaccttctc caagcagaga ataaagcagc aggttgccgg agccggagtt gcggtcgagt
     2101 tcatacatca acgtacctat tctcattccc gtagtaagcc ttacagatgt tgttgtactt
     2161 ccgtaaagct ttaaagattc cttcacggcc aaagtattta cagttacatt tgcaccgtag
     2221 cttacttcga accgcgcacc tgcctcaaca aaaattgttt cgaagtttat atttgcgtcg
     2281 ctatcaattt taaatgttct acctccgcaa acatccactt gactaatata gctgtcctct
     2341 gagccggtca atgtaagatt accggcacca ttgtcgttgt tgaccttgag ttcgatataa
     2401 tacagcttat gactgttaag gtcaacagta acagtatgat ttacatttat agttgttcta
     2461 tcatcgatat tcttataaat ctttacatag ttttcgctgt cttctgaagt cttgctaagg
     2521 caatcgctca gtgaatcaaa gccggttacc ttgtcgccta cagtcagcgt agcaacgaac
     2581 tcatactgac aggttttgca catacccgtt ttgctgtcaa catccgtatg accgcaccgc
     2641 ttaccgcaaa tcttgcactt gcccgttgtg ctgtcataac cgtgatcttc atgtcgtatt
     2701 acaataacag ttgttgcaaa accgtctttc aacagtgttt ttgagccgtc tatcggccca
     2761 ccggaaatcg tcataaacat acggttctca gccaacaaag tcagtatttc tgcgtttgct
     2821 ctgtccccgc cgccgttata gcgtattttt atgaaagttc cgccgtgaag aattacgtca
     2881 ctgcttgcat cgtttatatt gagtgttatt ctgagtgaat gatagcagtc aaatgtacct
     2941 gcacctaccg acacagttcc catagcgccg cagttttcgt caaatgtcag cttaccgccg
     3001 tccgctacat tgaaatcgcc ataaatatta ttattttttt ccgatcccgt aacggttata
     3061 tctcccgaaa cagttaacgt acctacattg gcttcctcag cgatccacag cgtgaactta
     3121 ccgctgagtg ttgtatagct gcctgtataa tttttgtata ctcttaatgt acagccggtg
     3181 tttacgccac tgtcagccgc ttgaaaagcc gcttccatct cagtttcctt ataaacggaa
     3241 gctacagccc cgtcctttac gataacagcc gcatactgat attcacattc tgtacactta
     3301 cccgtgtcaa tgtcaacatc gctgtgagtg catacatatc cacagccgca cttgccgtca
     3361 acataggtat ggctgtggtc tttgactgtc agatcactgc ctgactgaac acttgcgttg
     3421 aaaagatttc cgtcagcgtc aaagaaagcc ttaccctcgc cgagcatatc gtataatgtt
     3481 ctatcgcctt tgattgtaag atcgacatac ttaccggcat tcagtataac tgtaccataa
     3541 tcatttatat tgatcgcact gtcgaaatat atatcaccgt ctacgataag tgtaccgccg
     3601 ggagcatcga ctgtcggact taccgaaccg gagcatgtaa cctgttcgct gccgctgttt
     3661 ttaaacgtaa cctttcctcc actccagggg gtaacacccc tgacagtgaa atcgtgtccg
     3721 tttaagtcaa ttgtaatcac tccgtctata tcatgctgct caattttata ataatcctta
     3781 aacagcttta ctgtaccgct tttaccgtat tcattgagta aagagtgaag ctcatcggta
     3841 tctacatact ttgagattcc gtttgcatct gtatagattg cgacaga
//
LOCUS       DAWAOG010000147         3885 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2994_length_3885_cov_1.915144, whole genome shotgun sequence.
ACCESSION   DAWAOG010000147 DAWAOG010000000
VERSION     DAWAOG010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2994_length_3885_cov_1.915144"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    655..664
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    961..970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1570..1579
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2911..2920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1052 a          930 c          816 g         1047 t
ORIGIN      
        1 aaaacgagta tatagcaagc ggttttacca caatatattg aacgaaatac acacgcagaa
       61 atcgtgtata tcagccacgg attttgcgaa ctacatcaac accaagaaca gcattcagcc
      121 cggagccgtt atcccagcgg acgagcagat ttcctgcgtc gtcaacgcct ttaatcgtgc
      181 cgcgagtacc tatcggcggc gcctggaaat cgtccattga aaccagttcc acacggcagc
      241 tggcagggta ctctcggcgg tactgctcaa tctgctcctt actcggaaac ttcatttaca
      301 gcacctccat ttctgaaagc cgatgaacct gtgaggtttc tcagcagtat cttccgctcg
      361 gctttgtact ccgaaccgat gaaacctaac cgcaggagaa agcagcggaa tgcatactta
      421 tcgttgtcgg tttccttttc tttcgctgta actcgctttg cgtttgcggc aagttcgcaa
      481 agagccgata tgaaatgcgt gtaagccttt ctctgcggat cagccctatt acgaaaagcg
      541 ttacgaggat cattggctga atgaacgagt aatttgcaag gcttatagat ataaccgcat
      601 tttcaagcag cattccgagc gtatttggaa ttctggcaag cactacctta acaannnnnn
      661 nnnnactctc ggcagcatta tcagcgaaat tatcaccata gccggaagaa gctgcattgt
      721 tgatgaagca tacggagaga aactccttat aaccagcccg tacccgtatt ttgaaacaag
      781 atataacacc aacggcagaa ttatctgttt atagtttaca ctgccacatt tagacgactt
      841 tgcaataatt acaaggcctg cagctgttgc aaatatacaa atcagtttga aaacactgag
      901 ttcagacccg aaaaaaatgt cggtgaaata agacgcaaac agggttatcc cgagccacgc
      961 nnnnnnnnnn accagtaata ccgctaaaaa cgactgccat gtaaaactga aactcagatt
     1021 ctggaaaggc aggcttgcag caaggaacac tgacattgac gagcacatca ggaatgtaaa
     1081 ttcatcgccc gataagttgg ctttggcagc ggcgtatttg tcattcagcg agcatattgt
     1141 atagcaggct gtcacagcga gcatcagaat aacaacgttc ataatcgacc tctattatga
     1201 tgaataaaac ataccgacac gtttatttac ctgtccagaa tgcatagtcg gtccttgatg
     1261 ttccgtcgct gaaatatgct ttagcacata tcttgttttt cgtgccgggt tttatcttta
     1321 tatttttcca cacgaaaaca gtgctctcgt catcgggaag ttcgcaccgg gatatcctgc
     1381 cgtgcgaaac atcatttata caaagctcca ctgcattcgc atttgaataa acctttacaa
     1441 aaggcacatc acaagccctg aattcatgcc tgcgttcagt tatataaacg gttttttcgc
     1501 tgctccatac ggacttgtaa aagaaatata cgtccttggg aatacgctcc cttgtgcaaa
     1561 gtcccttgtn nnnnnnnnnt ataaattcgg cccacaggta ctttctgacc gagaattgtt
     1621 tccacaatat ctcgtgcagc tgcgactgat agttctcata atgccgtact ccgacagggt
     1681 ctatatcgct ttcccaattc acattgtcct tgtgctgcga aattgcccca ccgccgccgt
     1741 attcggaaag gcagacaggg cgcttttcct tttcgttatg gtataggtca agccattcgc
     1801 cgaaattctc cgcactgccg gcatccttat accagccaaa ataacggtta tacccgacca
     1861 catcagcggg aagctccaga aatctgccgt aaaactgatt gtctgcaaag gttttcagcc
     1921 ttgtatcgtc ctctttgcag gcaatttcat aaagctcgct gtaaagcccg aatatttcat
     1981 cggtcatctg gtgaagttcg ttggacaacc cccagacaat aactgacgga tgattgtaat
     2041 tctgcctgat aagctcacga agctgctgtt ttgcgttatc tccaaagccg tctgaaagaa
     2101 catgagcgtc gctgtcatcg gcagacattt tattgattat gcctatctcc gtccaaacgc
     2161 acagacccag cctgtcgcat aatgaatact catggtcaca atgctggtag tgagccattc
     2221 ttacggcggt acagcccatg tcacgaatga tacgataatc ccgctcacgc tgagcgtcag
     2281 tcattgccca gccggattcg taactgtcct ggtggtagtt taccccatgg agcggatatg
     2341 gcttgccgtt gaggaaaaag ccgttatcgg catctatgcg gtacgagcgt attccgaatt
     2401 cctgttcaat gctatcgacg ggtttcccgt tgcagaataa ggttatctcc gcacgataaa
     2461 gagcgggaga gtttactccg tcccagagtt tcgggcagtt gatttttccc gaaagcgtta
     2521 tcctgtcttt gtccgctctg ccgaaaaact ccgttacagg ctttccctgc ggatcaaata
     2581 tcacagcctt agcctctgcg gtttcggctt tatccagctt gacaagtatg cctatatccg
     2641 cagatgtttc tgaaatattg cgtggggtta tatatactcc gcaggagccg ctgtctttca
     2701 gagcaatatg aacaggctcg actgcaataa gctttacacc cctgtacaag ccgcccattt
     2761 ttgtgaaatc gccattgtcg gttatcggcg caatataatc ggtgggagca ttcgatacga
     2821 taacagctat cagattgtct ttgtccgggt atatatagtc cgtaatatca aatctgaaag
     2881 ctgaataccc tccgctgtgc tctcctgcaa nnnnnnnnnn tatatagact tgtgcaacag
     2941 tattagcacc ttcaaattcc agatagagtt cttttccgct gcattcttca aaactcagcg
     3001 caaggtttct gcggtagcat acagttccac ggtagtagtc cccaccgtct gcagctgcca
     3061 ttctgcctgc accgtcttca gaattatatg tatgcggcag tacaacaggc tgccatgacg
     3121 catcttcata ttcgggagag ataacgttgt ctttcgatat attcattcct gccggaagct
     3181 ttgtgaatct ccagaaatcg ttgagtgcct ttactgttct caaaaaaatc accctttctt
     3241 gttttaatag attataacgc ttattgacaa acaagtcaat gaatgatatt attaagatat
     3301 aacgatttta gcacaggcgg tgaattaatt ttgaaattct acgattatcc gataatcagc
     3361 aaagaaagca gtcttcccgt ctttttggtg agcgtagggc tgaacgaatg gcagtatcac
     3421 gttaaatgcg acaaaggtga caattatgcc aaggctcttt attgtacaaa aggcagcggc
     3481 gtacttataa tgaacggaga gaaacatata ataaatccgt acaccgcatt ctttatccct
     3541 gccggttgtc cgcatgagta ttattcaacg gacgaatcct gggatacgca ttggataaaa
     3601 ccgtcaggaa agtcctgccc tgatatgctt aaagcactcg gctttgacaa gccgaaaatc
     3661 ttccctcttc caaaggaaga aattacatat cttgaccact gtttcagatg tatgcacgag
     3721 gcgcttcttt ctgacaaagt tgacggaaat ttcagagcgt cgggctatct gtacagcttt
     3781 ttcattgaat tgagccgcct tgcggcgaaa ggaaagggca gtgtacaaat tgcccctgca
     3841 gtgacaaagg cgatcgcata tattgacgaa aactatatga aacag
//
LOCUS       DAWAOG010000148         3883 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_2997_length_3883_cov_2.653344, whole genome shotgun sequence.
ACCESSION   DAWAOG010000148 DAWAOG010000000
VERSION     DAWAOG010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2997_length_3883_cov_2.653344"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1002 a          904 c          887 g         1090 t
ORIGIN      
        1 catcttgacg gggtcggttt cctgctcacc tatcttgtat gctacagtag gagcggtagg
       61 tgtcagtata acgtccgcat tctcgaatat ctcgttatat tcctttataa tctgctgttt
      121 taatgccagc gcctttaaat agtatgcgtc atagtaaccg cttgaaagtg cgtagttacc
      181 gagaagtata cggcgcttta cctcatcgcc gaagcctctt gtacggctgt cgcggatcat
      241 ctcgtcaaag gttctgccct cgcctcgaag tccatacttg atgccgtcaa agcgtgaaag
      301 gttggaagcc gcctcggcac ttgccacaag gtagtaagcc gatacagcgt acttaaggct
      361 cttcatcgag cagtcaacga gcgttgcgcc catttcttca tatttctttg ccgccgcaag
      421 gactgcgttc ttcacttcat cgtctgtgct gtctgcgaaa aattccttcg gaagagctat
      481 cttcataccc tttatatcct tgccgacctt ggcgttgaag tcctcggcgg gcttgtttgc
      541 gcttgttgcg tcacggctgt ccttgccgca gataacgtta agtatcgtac cgcagtcgac
      601 cgcattgttt gcgataggtc ctatctggtc aagcgagctt gcgaaagcca ccagaccgta
      661 acgtgatact ctgccgtatg tcggcttaag acctgtcacg ccgcagaatg aagcaggctg
      721 tcttatagag ccgcctgtgt cgcttccgag cgccgcaggc gcaagtcctg ccgatactac
      781 agccgcagag ccgccgcttg aaccgccggg tacacgggat aagtcgaagg gatttcttgt
      841 ctttgcaaat gcgcttgtct gtgtagagcc gcccatcgca aattcgtcca tactggtctt
      901 gccgagaaga acataattct cggccttcag cttttctatt acgtttgcat cataaggcgg
      961 aacaaagttt tcaagcatct ttgatgcgca ggtcgttctt attccgtccg tgcagatgtt
     1021 gtcctttatt gcaagaggta taccggtgag tgcctttgcc tcacccttat ctataatctc
     1081 ctgcgccttt tccgcatttt tcagtgcctc gtcatcggtc actgtgatat atccgagtac
     1141 atcgggatct ttagccttta tgctgtctga atattccttg caaagctctg cggcgcttat
     1201 ctgctttgtg tcgagcattt cacgcagaga acccagtgtc tgttttttaa gctccattat
     1261 tctcttatcc tttcagctcg tttatcagtc taccatcttc ggaactacat agcagtttcc
     1321 gctgggctgt gtgttgctga gcagcttctc tgtggggaat gagggctttg caacgtcttt
     1381 tcttacctcg tcatagctta tgctgttatt atccttggta tcatcgtaaa ctacgtcaaa
     1441 ttcctttacg gtgtccataa gctcgattat cgcgctcatt tcgcccatta ccttttcttt
     1501 accctcatcg gtataattga gcttgctcag attacacaga tggtcaatgg tctttatatc
     1561 cattatgccg tcattccttt ccttcggggc tttttctgcc gtttgtggca gaacctactg
     1621 acccattata gttatacttt aactattata caacattgcg agcctatttg caagcgttgg
     1681 agggtaaaaa actgtgttta agtgagattt gaaaacacta ccgaggtatt ttagtatact
     1741 attaaagtat aattaccctg ccttgtccgc tttagcaccg gttgaaatat aagcgtattt
     1801 actgtttttt tcaaaaccgc cgtcaacgta acattcaaaa ataagcaaca ttgggaaagg
     1861 caaatttaca ttgcctttca gacacacaag aactcccccg acctatcatc gggggagttc
     1921 ttgcattgat tgaggggatg tatataaact ttcgttgtac ttagtcggag cggtatcgcg
     1981 tctgcggtaa ctgcgcccct ataataaccg ccatggcggc aatcaaggca ctcgccttat
     2041 cgtcttcgga aatccttttc ctctgactat ggctaaatta taacacccgt ttgttaattg
     2101 aataataact gagggtgaaa aaattatgaa aagaagaaat tccccgactt ttttcgtttt
     2161 ccctattgac aaatcgcccg tatcagtata tactgtatat ataacacata aacagtatga
     2221 ttgcctgtta tgtgatgtaa gcttacaaca ccaaaaagga ggaactatga aaattcttct
     2281 taattccgat attccgatat acaagcagat ctcggccgat ttcaaggaac gtattatgaa
     2341 cggcacgatt tccgccgggg aatatcttcc ctcaataaga ggacttgcaa aagagcttaa
     2401 aatcagcgta ataaccacta tgaaagcata tgaggagctt tgctcggagg ggcttgtcag
     2461 tgcagttcag ggcaagggct atatagtcaa cgctcaggac acgggtatga taaaggagca
     2521 gtatatgcgc cttcttgaac agcatctgca ggacgcactc gactgtgcaa ggcttgcggg
     2581 gctgtccgtt gacgaggtat gcgaggtgct gaaaacactc ggcagtattg aatgatttat
     2641 ttaaaaggag ataatatgga agaggtaatc aaggtaagtg atcttaccag aaaatataaa
     2701 gatttttcgc tgggacctgt tgacctttca ttcccgaaag gcttcgcaac ggcgcttatc
     2761 ggtgcaaacg gtgcaggaaa aacaacactt atggatataa tctgcggcat caccgcaaag
     2821 acagacggca cagtaacata tttcggagaa gccgacaatg ccgacaaggg taatgtaaag
     2881 gagcgcatcg gctactgtgc atcacaggcg atgttcccgg ctaactggaa ggtgagggat
     2941 attgcacttt ctatgagcct tgcgttcgat aaattcagca aggacagatt ttattcactt
     3001 ctcggtgagc ttggcgttac cgatgagaaa aacagtaaaa agcctttatc aagtatgtcg
     3061 gacggtatga agatgagagt gttcttggca tcagcttttg caagagaaac cgatctgctt
     3121 gtgcttgacg agcccggctc atcgctcgat ccgcttatgc gtgacaggct gtgcgaccgc
     3181 ttccgtgact atatagacag cggtaacggc gaaaggagta taatcttctc gacccacaac
     3241 atagctgata tggagaatgc cgcagactat gtaatattta tggataacgg caaggtaatc
     3301 gaacagggct tcacagagga cttaaaggac aagtatattg ccctcagtgc cgagcctgaa
     3361 agccttgaca aaatatcgcc gcttctgctc tgctcttccc attcatcgtc cacagcaaca
     3421 ggtcttgcct tagccgaaaa caagcaaaag ctcgaagcac tgggtgctgt tacggaacgt
     3481 cctacattaa gccaactcag cattgacctt atgaagattg cggaagaaaa acgccttgct
     3541 cttaaaaagg aggcataagt atgatgaaat cggtacgctt attcaatgct aacacgctgt
     3601 tcaaggtttc cctcattatg attgttatgg ttgcggcaat ttatgttata ggcttcttag
     3661 tcggctctgc cgcaggaaaa agcgacagag aaaacactga cgacagcaca gctgtcgcag
     3721 aggaaaatga cgatattgca tattccgccc ttaacacagt gtgctgtgtt atcggctttg
     3781 caggtgcgct attaataaac ggcaacgcca taaaccttta ctacaagggt gacggcagta
     3841 agtacgcaag aacgataaag cacggcggag aaaagtttgg caa
//
LOCUS       DAWAOG010000149         3845 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3031_length_3845_cov_2.262005, whole genome shotgun sequence.
ACCESSION   DAWAOG010000149 DAWAOG010000000
VERSION     DAWAOG010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3031_length_3845_cov_2.262005"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          916 a          866 c          936 g         1127 t
ORIGIN      
        1 gagccataca tttaaaattg acctcacctg ttttaagaac cagccttaac agcttgtcgg
       61 gatcgttttc gccctctatc gctctgagtg ccgtgtagaa gaaagagttt acctcgctgt
      121 ctgttttgcc gagcgcaagc gcgtgataag cgtatgccgc catacctttg ataccgaaca
      181 ggataagcga tttcagcgaa cggatatcat cgacagcgtc ccacagcctt gaaagctcat
      241 agtcgtcaac gtggctttta tatatcctgc gcttttcgtc cttgacctcg gctgtaagct
      301 cctttaccgt atgggtattg aagcttacgt tagttatggt tgtgaacaga ccttttaata
      361 caaggcggtc ggtactgtcg gtaggcgtgc cgtactctgc cgccttagca agccctataa
      421 gcgcgccgat aagctcgtcc tgagcctttg cggtatcgct tttcttgccg cagacacctg
      481 ccttaccgct gcatttttcg ttatgtgcag tctgctgaca ctgaaaacaa aacatctcgt
      541 caatcattgt tttacctcca tcggttagtg ttactttatt tttacttcgt taacaagcgg
      601 tttgcccgca aagaaagcat caaggttttc aaacgtggtt actgcgatat tgaagagcgc
      661 ttcgcgggtg agaaaagcct gatgagaagt gagcagtaca ttcgggcggg agataagcag
      721 ggcgagcaca tcgtccttga ttatcgtatc tgacatatcc tcaaagaaga agtcgccctc
      781 ttcctcgtat acgtcaagac ccgcacctct tatctttccg ctgttcagtg cgtcaagcag
      841 ggcagtgctg tcgataagcg cacctcttga ggtgttgatt ataaatacgt catttttcat
      901 ctttgaaaaa gcgtttttgt caagcatacg cctgttcttg tcggtgagcg ggcagtggag
      961 cgatattaca tcactgttgg cgtacagagt atcaaggtcg gtgtagataa aatcgacatt
     1021 ctttgcgggg tatgggtcat atgccagcac gtccataccg aagccgtggc agatatctat
     1081 aaatgctctg cctatcttac ctgtaccgat aacgcctgcg gtctttccgt gcaggtcaaa
     1141 gcctgtgaag cccgctatat tgaaattgaa gtcacgcaca cggatatagg cgcggtgtat
     1201 ctttctgttc agggaaagga gcagtgccat tgtatgctct gcaacagcat aaggagagta
     1261 ggcgggtact cttactatct ttatcctgtt ctgcgccgct ttcaggtcaa tgttgctgca
     1321 gcccgcactt cgcatcgcta ttatctttgt gccgccgtca cacagcgcat caatggttct
     1381 gctgtcgata tcgtcattta caaatgcaca gaccgcttcg cacccattag ccattaccgc
     1441 ggtgcggcag ttcagctttg attcgatgta tatcatctca tatcccgtat tgacattatc
     1501 gaatgacgct ttgtcatatg attttgtgtc gaaaaaagct atctttttca taggctatga
     1561 taatctgctg tcttcctttc gctgaaaata gccttggggt ctgcggcaga ccggacagta
     1621 ttcgggagga gtaatgccgc agtgtatatg accgcagtta cggcacagcc atactgtttc
     1681 acccgaagag gagcgtatgc ttccgtccga gagcatttgt gcaaatgtgt tgaaacggct
     1741 ttcgtggtct ttttctatag cgcctacgag ctcaaacaga tctgctatat tgtcgagctt
     1801 ctcctgcctt gcggtttcgg catattcctt atacatctgc gtccattcgt agttttcgcc
     1861 gtgagcggct ttttcaagtg cttcggcggt tttcgggata cctccgaaaa taagtccgaa
     1921 ccaaagctct gcatgagctc tttcgttttt tgctgtggtg cggaatacct gtgatatttc
     1981 ctcgtcgccg tcggactttg ccttgtcggc gtatatctca tagcgtacca ttgccgccgc
     2041 ttcatttgca aatgcggttt tcagattttc aagtgttttt gaatcggata attgcataaa
     2101 aaatctcctt tgctttagtt accgttattg taaccgaaga aaaggagaat atttattcaa
     2161 attttgtttc gcgggaaaaa aggatcactt tcttatgaaa agtacgccga gagtgttcgg
     2221 tccgcagtga ctgcaaactg tacagccggc atctgtttca agtatctctt caaactttcc
     2281 gagctttttc actgccttgt gggctatatc gattatttct ttatcgctgg gtgagtgggt
     2341 gatgaatatg cgcttgtaat caatatcatc tctgtctgac aggcgctcgg tgatatattt
     2401 ttctatgcag gcggaatatt tacctctgta cttcttgccg acacccatct tgccgtcctt
     2461 tacttctatg cagggacgga gcgaaagaag gttagcgccc agcgccgcaa gtgtagagca
     2521 tctgccgcct ttacgcagga aatccagctt ttctattaca aagcttgctt ctacctttga
     2581 tgtaagagcg ttcaaagcct ttacgatatc ctcgggagct tcgcctgcct gagccatctc
     2641 tgcagccctg agcactataa gtccgctgcc tgttgaaagg ttgcggctgt ctatgatata
     2701 gacattgtcg aaattttcag cggcaatgca ggcgttctgg taacagcttg aaaattcggc
     2761 gctgatatca acatggatga ctgcatcgta ttccttgctg agaggcgtga atgcttcgat
     2821 ataatccgct acgtttactg cagcggtaga agtcaatgca cctcctgctt ttacatggtc
     2881 gaaaatatcc ttcggggtga tctcaagtcc gtccttgtag gatttgccat ccttgataat
     2941 gtaaagcgga agaattgtaa taccgtactt ttcgacaagc tcctttgaga ggtcgcaggt
     3001 gctgtctgcg gtgatcttta tattcataga tatgctcctt tcgcggaaaa cgttgaattt
     3061 aatagtacta agtataacat attttgtacg aaacggcaat agttttattc cgtttcaggg
     3121 agaaattata ggaattgaat aaaactactt gatatttcaa aacaattatg gtaaaatgat
     3181 gataatcaat ttttcggagg taattttatg caatatgaga tcaaaggtac accgctgcct
     3241 gttgtaatat gcaatgtcgg tgcgggagaa tcaatgtata ccgaaaaggg cggtatgagc
     3301 tggatgacac cgaatatgca gatgtcaacg aatgcgggcg gcagtttcgg caaggcaatg
     3361 tcgagaatgt tctcgggtga gtcgatgttc cagaatatat acactgcaca gggtgcaccc
     3421 ggtcagatcg cttttgcgtc aagctttccc ggtgagatac tggcaatgga cgtgtcgcag
     3481 ggcgctgtta tagcgcagaa gagcgcattc cttgcggcgg agatgagcgg caatatgagc
     3541 atacatttcc aaagaaagct cggaagcggc tttttcggcg gcgagggctt cataatgcag
     3601 aggttcgagg gcagcggtat ggtgttcctt gaaattgacg gctcggtaat tacatatgat
     3661 cttcaggcgg gacagtccat gcttgttgac acgggatatc ttgcggctat gactgaaggc
     3721 gtttcgatag atatcgagag cgtaaaggga ataggcaaca agctgttcgg cggcgagggc
     3781 ttcttcaata caaaggtgac gggacccggt agaatatggc ttcagacaat gcctgcttct
     3841 gcagt
//
LOCUS       DAWAOG010000150         3832 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3046_length_3832_cov_1.848028, whole genome shotgun sequence.
ACCESSION   DAWAOG010000150 DAWAOG010000000
VERSION     DAWAOG010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3046_length_3832_cov_1.848028"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1654..1663
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2130..2139
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2919..2928
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1001 a          857 c          647 g         1297 t
ORIGIN      
        1 tccgcatact gtgccaactc agaaatgtac ctttctgtcg cagacagcat ttcatttgtc
       61 ctgataaaca tatcttcgtt tacagagcat tttttcaaat gcgcatataa tttagaaata
      121 agctccttac ggttcatttc aaacggtaca aattccgtta tcatcgttgc ttgtttagca
      181 aatgatatta gtttttcgtt gcacgatata actgaaaatc ctgttcctcc ttctatctga
      241 ttgtttatat cattcagcac cgtgtaaaag aaatccggat gttcaattat caacgtcagg
      301 actttatcct ccgagtcaac atataattcg atttgcgaat aggcaaacgt cataaaatca
      361 ccagcctttc gtcgctgtct ataacatcgg atacaaattc gcctgtaata tattccatat
      421 ccgcaaattg tttttcagtt atacacagca aagacacaat acccgcagga ggactgttgc
      481 gtttgattcc gtcggatatc agtcttgccg ctgtgcgatt caacgccata cggcagtaaa
      541 ccgattcctg aaacatcata aaaccgcttt tgatcagata ttttctgaat tttgtgtatt
      601 cccggcgttc ctctgcggtc gtaaccggca aatcaaaaag tacaagtaat ctcatatatc
      661 tgtaactcat tttatctccg ttttgaaatc ccgaacacga taatatctta tcagcgaaac
      721 atcgttttcc tctattgcgt caaatacact tctgcaataa attttaacgg catttgtaac
      781 atactgctcg gtattattga tcattacgct ttcattcaat atccctatga tctcacgttt
      841 ctgctctgag gtaaaaatgc ggtagttctt ttctctgaca aaacgatcta tcagcgttct
      901 gaacggctcc atcagatcag agccaagatt aaaattgttg aacatattat catggaacag
      961 accaagctgt gtaatgtatc cgttagatgt tatctcacga ttgaacgctg aaagtattat
     1021 actgtatccg tagttgagtg cagaattgat tgcgttatcg gcatttcttg aaaactcttt
     1081 tccgaaaagc gcattgaaat atacttttgc ggcatgaccc tccctgttgg tttcatcgcc
     1141 gagttctatt tcatcaatat attccataag catttcagat tcttttgtgt ggtcaaattc
     1201 ctgaagaacg cgagcctgat tgcgtatttt ttcactgact atacttgtcc atatatatgc
     1261 tttgacatct ttgtcccact ttacctgttc gcgcaccctt gccgtacagt tatgacaccc
     1321 atataaaggc agcatttcgc acgagggatt gtgcttctcg tcacagaata tcaatttcac
     1381 cttgcgattt gcaagctcgt tgataagact tgcagttatc gacaccgcac aattctcaat
     1441 tataagcacc gatatctcgt ccaataatac acgcttgata ttctcttcgc ttctcacgac
     1501 catgaagcca agcttcttat ccagcttggc tcttccggtc acaattactg ttctccagct
     1561 catagctcaa gtaagttgtc agacttattt tcgtataagc ctgtcggcga ctgatcaata
     1621 attctgatat cgctgtatcc cgtgttactt agcnnnnnnn nnngccttca gctgatcggt
     1681 aagctgcagc ttttcaaact tttctcttcc gtttctcatt atttctcctg attcaacaaa
     1741 ctttttcaaa gttgagaacg gctttttcaa gcacttttct gttatttcat catatactag
     1801 caaattctgc tgcgcgtcga agctgtttcc gggtttgtat ttcgctccgt tttcactttt
     1861 ttccaagtat gatacaacag acttgatata gcttgctgtt ttttcggaat atacagccga
     1921 catagctgaa gatgtgccta catatttgcc tttgctgtct ttggagcaca tatttattct
     1981 atagccgtcc acctctaaaa gagtgttgat ttttatcaat ctcttattca gcaggaaaac
     2041 tatatcgctt tcgctgattt tgccaagagc cttctgtgaa actatatttt tcatttgttc
     2101 aagcacatat ttccttgcaa actcatcatn nnnnnnnnng atttaggtac agttataata
     2161 cctgtagatt tgctttggaa aaatatattc ttttttccaa agcaaatcta caggtattat
     2221 aactgtacct aaatcaccgc agcgtaccaa agaaaagcaa gaagcgcttg cactgttata
     2281 ccctccgtat ttcaccgggt cgagattttt ctttagtctt gcaagatccg ggttaccggc
     2341 tctgtcagga ttctgattaa agaatcctcc ctttctctta tagcagtaac gtacatatct
     2401 cacgctgttt ttgctcatca tacgcttaac cgtatcaaaa tccgtttcag gattccatgc
     2461 cgtttcaccg tttcttgcaa ccttgtgctt aagcgtttcc ttgatcttga tcgtatagtg
     2521 cttatctgca ttttttacaa agtttaaagg atttgatgta aatttgacat catgaacatt
     2581 accgacaaca acattcagat aagcgtcctt tgcatggtgc aggtcgttta tctctctgca
     2641 tttcagcata tcaaagtcat gtctgaactc tgaaacctgt cctgctttta cataaacgat
     2701 cttactgtca gggaacattt ctttaagcag cgttgcaaca gcctttgttg actgtcttgt
     2761 ttcaacaagc tgacgttcaa taaatcctgc gagctcatct tcggtaaacg gcgttgaacg
     2821 tgtcaatctc tcatatttta tcttatctgt cggattttta ggatcacgat aaaacaggtt
     2881 tttccaaatc ggatgcattt tatttcttat ctgctcttnn nnnnnnnnat cggataatcg
     2941 tccgacttct cgccgttgag ctgtgatttt acaagtacct tattatgaag tgaatcatct
     3001 ttaactttgc tctgaggata gatatgatct atatcataac agtggttatc tccaaggcgg
     3061 ctgaaatcta tcgcttcgcc ggaatacaca cactttccga actgagtaaa atacagataa
     3121 tatttctcac ttctgagctg ttcatcggaa agactgccaa gctgtgatct cagatgcgat
     3181 atttcttcat cgctgacaaa ttcttttgcc gaagcaaaca atttctctat ctgttctctt
     3241 ctcgaagaag tgcgttttcc tttgtcttca ggcttgctgc ctcttgccat ttcgacaaaa
     3301 attttatcgg gatctctttt aattatctta cggagttcct tgacgatatc cattgttctg
     3361 ataatagctc tggatgccga cggtgaaaca tacatttcac gaagagtctg tgcaatgctg
     3421 cccgtaggat tgatcgcata atactttttg ttttcttctt ctataaagct cttgtagccg
     3481 tatgaatcag acatgatctg cattaggttg atgttctcag cccacataag atcaataatg
     3541 cttctgccac cgatctcacc ggtatccata tccagctcat aacagcctgt aagcatttta
     3601 gctgataatc tgccatagtc cttatacgaa agacgactga tatacctgat atcctctgca
     3661 ggtaaagcgg gatactccgt cttgagccat gaaataagtc ttggcttatc tgtagtaacg
     3721 gtaatacgct cgattattct ttcaacatcg ctttctgaaa gagatccgtt ttcgagtaac
     3781 ctcctgaaat ccaggtatga cttcagcgaa gactttatgt taatatcaac tc
//
LOCUS       DAWAOG010000151         3832 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3045_length_3832_cov_2.399788, whole genome shotgun sequence.
ACCESSION   DAWAOG010000151 DAWAOG010000000
VERSION     DAWAOG010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3045_length_3832_cov_2.399788"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2897..2906
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1124 a          933 c          885 g          880 t
ORIGIN      
        1 ctctgttcga cccgggggac tgtacagata tctcaggata gtgcgttttt tggaaatttc
       61 ggtgacagtg cagttgtaat gatacactca cgaaattatt aacgccttgt tggcacggat
      121 actgttttca gcgttgttgg ctgtatatct attacgccga cttttttaca atatgttgca
      181 ctgcaatttc aatgtaattt tgccgcgccc cacgcaaatt tataacactc tcctatacac
      241 gcactgtcaa ttcccgccgt tgctctatcc cacaccgtgc gccgcctttt ccaaaggcgg
      301 aatttgccgc gccacacgca aatttataac acactccagt acgcacaccg tcaattcccg
      361 ccgttgctct atcccacgcc gtgcaccgcc ttttccaaag gcggaactgt cagcggcgac
      421 acgccgcttg atttttgcgg tatttatggt atacttatat cagacagcag tacaataagc
      481 atataaaaca atatgatccg ctcgaaagga ctaatatggg atttcttgta atagacggca
      541 acagtatact taaccgcgca ttctacggca tacgcgtact cacaaattca aggggtgttg
      601 ccacaaacgc cgtgaccggc tttatgaata ccctgctgat gctcgaaaaa gatgttcagc
      661 ctgatatgat agccgtagca tttgacctta aagcgcctac attccgccat aagatgtatg
      721 acggatataa ggcgaaccgt aagggtatgc ctgaggatct tgcccagcag ctaccctata
      781 tgaaaaagat aataaaggca atgggtatcg ccattatcga acaagagggg tatgaagccg
      841 atgatattat cggcacgatc tcagccgcct gcgcggataa aaagattccc tgcaccgttt
      901 cgacaggcga ccgcgactca ttccagctcg ttaatgatta cgttacggta aggcttgcca
      961 aaaccaaagg tgatatatac tatactcccg gtgtaataga agacgaatac ggcgttaccc
     1021 caaagcagat gatagaggtc aaggcgctta tgggcgatac gagcgacaat attcccggcg
     1081 ttcgcggcat aggcgaaaaa accgccctgt cactcataaa agagtttgca agcgttgacg
     1141 gcttatacga aaacatcgac tcgaccctca taacagacgg cttgcgcaac aagctcatca
     1201 acggcaaaga aagctgctat atgagcagaa agcttgccga aatatgcctt accgctccga
     1261 tagacacaga gctttctcac tatattccgg gtgagcgtga cgatgaacgg ctcgcaaaac
     1321 ttttatccga gcttgaaatg tataagatgc ttcagaaatt acagcttcaa ccgacttctg
     1381 cccccgcagg ttcaaaagca gcaggtaccg gaaatcgtga aaatcaggct tcagctatgc
     1441 ccaagggtga tatcatcatt acggatagca gcaaaattta cgcaggcggt atcggcataa
     1501 tgaatgagct gtccggcgat gagctcagag catacgcaaa cagcggcagt acaaagtata
     1561 ccttcgacat aaaggaaacg ctctccgttt ctggtcttga gaagcttgaa aataataagt
     1621 tcgatacgac acttgcggct taccttgcag accccgactc aaacgactac agtatgtcaa
     1681 gactgtgcac acagtacggc gttcccgagg gaaacagcat acaggaaaag tcgattactg
     1741 ttgcggcact gaacgatata ctgtacgaca atatacacca aacaggctca gctaccgtgc
     1801 ttactgatat cgaaataccg cttgccacag tccttgtagc tatggagcgc gagggcgtta
     1861 agctggacat tgacggtatt aaagctttcg gcgaagaaat ttcggaaaag gcagaaaaga
     1921 taagccggga gatctatgaa tatgcgggct atgaatttaa catcggctcg cctaaacagc
     1981 tcggaagcgt gctttttgag aagcttaagc tatccagtgc aaaaaaaaca aaaacaggat
     2041 actctacaaa tgccgaggtg cttgaaagcc ttatggacaa gcacccgata atcccgctta
     2101 taacagagta tcgcgcactc accaagttgc agaatactta tgtcacagga ctgcttaagg
     2161 tagtcggcga ggacggcagg gtgcattcca ccttcaagca gacagaaaca aggacaggaa
     2221 gaatttcaag cgccgaacca aatattcaga atatcccggt aaggactccg ctcggcagag
     2281 agatgagaag atttttcacc gcaaaggacg gatatctgct tgtcgatgcc gactactcgc
     2341 agatcgaact gcgcgtactg gctcatatct caggcgatga aattatgaaa aaggcgttcc
     2401 ttgacggcgt tgatatccat accgttaccg cctcccaggt gttcaatcag ccgattgaat
     2461 gggtaacgcc cgatctcaga agcaaggcaa aagcggtcaa ctttggtata gtctatggta
     2521 taggtgcatt ctccctgtca aaggacatcg gcgtttctat gactaaggca agcgaatata
     2581 taagagcata tctttcaaaa tattcgggca tagctcacta tatggacaag accgttgcaa
     2641 aggcaagaca tgacggttat gtcgaaacaa tgttcggcag aagaagatat ataaaggagc
     2701 ttgccgcaaa gaacaagaac ctgcagtcct tcggcgaacg tgtagctaag aataccccca
     2761 tacagggcac tgccgcagat atcataaaga tagcaatgat aagggtctac aacaggcttc
     2821 gggaaagtaa gcttgacgca aagcttatac tacaggtcca cgacgagctg atagtcgaag
     2881 ccaaagagga ctgcgcnnnn nnnnnnacaa ggcagccgca cttttaaagg aagaaatgga
     2941 gaacgccgta aaactcaccg tacccatgac ggtagacgtg aatatcggta aaacgtggta
     3001 tgatactcat tgagaaagat aacaacacgg atttatgccg cgatatcgga gtgatcgaaa
     3061 aagaatgctt ctccgtcccg tggacaagcc gcagtatcga gtcgcaggtt ctgaccgatg
     3121 ggtcggttgc cgcccttatc cgtgacgacc gctccgacaa cagacccgtt ggctatatct
     3181 gcgggcagag cgtttcggac gaatgcgaac tttaccgcat agccgtactc cccgcataca
     3241 gaaaacaggg tgcggcggat cttcttatga cctattttct tgacaagtgt cgtgaaagcg
     3301 gcataaaaaa cgtatttctt gaagtgcgct gtgacaacga gcccgcaaag cgcctttatg
     3361 aaaaacacgg cttctgcgag gtcggacggc gcagaaacta ttatacagag cctatctgtg
     3421 acgctcttat ctacaaagcg gaattgtaat taaacataat ctaaagcgac aggcacacct
     3481 attcacagcg tctgtcgctt tgttttacaa tatgcgaaaa cggcaggcac gaattacgcg
     3541 cctgccgtta ttacttattt gcttatgcct ccgacagaag ctttacaaag ccgatcgtcg
     3601 ctgtacacgg aatatccgct ttacacgata ccatctttac ttttgcctgt cggaaatata
     3661 catctgcatt acgcccggtt aaaaacgatc ccacaaaact tgcagtatca ttcagcctcg
     3721 ccgttttgct atcctgcgga tagctcagac aatgccaaag gcggaatttg aagcggcacc
     3781 ctgccgccac gccgtttcac tatcctgcgg atagctcagg caatgccaaa gg
//
LOCUS       DAWAOG010000152         3813 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3056_length_3813_cov_2.830761, whole genome shotgun sequence.
ACCESSION   DAWAOG010000152 DAWAOG010000000
VERSION     DAWAOG010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3056_length_3813_cov_2.830761"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1039 a          921 c          891 g          962 t
ORIGIN      
        1 gtcagcgcct ttgcaatgct gtttcccttg cggaatacta tccagccgcc gcaataaagc
       61 accggcaaag tcacaccgaa tataagatac agtgtctgcg cgccgtcata tatgtacccg
      121 agcgttacaa acgggagctg tatgaacatc aatataaaac agccgaagcg acctatctgc
      181 tcacctgttt cgacaagctt gttgtgaaat tcacccgcaa agccgtcctt gtttgtcaca
      241 gcatagatca tattaggtat caacaggaca acgacaaata tcagccctgt gagattaaac
      301 cattccatct tgccgcactc ttacgcaaca ccgaagccga gaatttctac atatgttgtc
      361 ttatcgtcac cgctctgcgt aacaggcttt atgctgacct tgtgcgttcc ctccgcaccg
      421 gaatatgcaa gagttgcaac gggattgctc cagccgtcgg tcgcacttgt cgcaatagtt
      481 gtgtagacct ctccgtctac cgtaaactgc aatgagccga acacggcgct gttattgcat
      541 ttatagacaa gcataagatt tttgccggtg aactcaagct ccataggctc acagcccgag
      601 ggcttgccct tgtagctcca gccatccggg aagcttgtga gagtttccct ttcggtatca
      661 aatgtgcctg ttgaagatat tttcagctta tcggaggaat gcgtcctgtc gataaatgtc
      721 atatccgcaa atctgtcgga atatacccat gtatcgggca gaggagtgat agttacatcg
      781 cccattgtct ttatcttttc ggtcgccttg tcaaacatat tcataataag gtcggcggtc
      841 atcttgtgtc cccactcgtt aggatgcgat tcgtcatcgg aatagtcctc ccacttcatt
      901 ctgcctgttt caaactcgtg ggaaagtacg ttgttgagcg acaccatagg cagaccgtaa
      961 gccttgccga cctgcgacat atattcctcg caggtgtgac cgctctttat gaccgtaaag
     1021 tacagcgcaa cggcaggctg cttttcctgc gaaaggatct ttctcaccat agcgttatag
     1081 ctgtccttgt atatctgatc gtttccgtca tttacggcaa actctacaaa taccatatcg
     1141 gggtcatgat ccagcacatc gcgctgaaga cggatatttc cgagtacgct cggcgtaccg
     1201 ctgagccctg cgtttacata gtttatcttt gtgtcggggt acttttcgca aaggcggtca
     1261 tacgtcagct tcgcccagca cttttcgggt cctgccgtaa gtccctcggt tatgcttccg
     1321 ccgataaatg ctatagttac ctcctgcttg ttttcaagct tttcgataaa tttcgacatt
     1381 ctttcaaggt tgccgagatc cataagcgac ctttcgacca tattgtcgta gatctgctcc
     1441 tcggtaaggt cgctttcatc tatactgctt gtttcagatg aagtatccga actgccgtcg
     1501 gtagattccg atacagatga tgttgtctgt gtatcggacg agctgtccga tgatacggca
     1561 gaaccgccac ctgtgctgtt atccgcacag cctgcaagca ttgacgcaca cagtaccgca
     1621 cacagtgtta tcttgatttt cttattcata tttacctccg tcagaaaaaa tatgtttata
     1681 ctattataca ataatcccga ccgataagca acagcttttc acaaaacgag caatttcact
     1741 aaaatccgac tgcaataata tactatataa accgaccgcc ttacaaacgg tcagttgttg
     1801 cataattccg tacagacggt taaaaataac cgtgtacatc agatacgttt aaagcatcgc
     1861 atacgaacag aaaggaagaa aaccatgctt gtaaaaagag gagagatata ctacgccgat
     1921 cttagcccgg tggtcggaag cgaacagggc ggtatgcgcc ccgtacttat tgtccagaac
     1981 gatatcggca accgccacag tccgaccgtc attgccgccg ccataacaag ccagaaggac
     2041 aaatcacggc ttccgaccca catttcgata aatgccgacc gttgcggtct tgcaaaggat
     2101 tctatcgtac tgctcgagca ggtaaggacg ctcgacaagc agcggctcaa ggagcgtatg
     2161 ggtgagcttg atgagggcgc tatgaacaag gtagacagtg cactgtcggt aagcttcggt
     2221 ctttcgggaa gcaattaagt aaatatcccc gcgctttacc ttaacaatat gtaacaaagt
     2281 tacacaccgg cttatgttta ggtctatgta atggttatca tcgccgcttg ctttagctat
     2341 tgctacaaaa ataaatgcac ttatgctttt tttgttgtaa aatcgtcatt ataatggtac
     2401 aataatcacg cagtaaagca aaaatgacgg aacggagaaa aacaatgaac atagctattg
     2461 cacagtcggg cggacctacg gcggcgataa actcttcact ctgcggagtg tttgacgaag
     2521 cggaaaagta taacgagatc gacagaatat acggctcgct gaatggtata gagggcatac
     2581 tgaacgataa ccttatcgac cttaaggaag tgcttatcac agaaaacgac aaacagctac
     2641 tcaagcgcac accgtcaacc gtgcttggtt catgccgttt caagctgaaa aacagcagtg
     2701 atgacgacag cgactacaag cgcataacag aagtgttcaa aaagtacggt ataaccgcat
     2761 ttttctatat cggcggtaac gactctatgg ataccgtaat gaagcttgac gcttatttca
     2821 gagaaaacca cattgatgta aaggttgtcg gtgtgccgaa aacgatagac aacgacttac
     2881 cgctcaccga ccatacaccg ggctttggat cggcggcaaa atatgttgcg acttctttgc
     2941 aggaaataat ccgtgacagc cgcgtatact caattccgtc cgtcacaata gttgagataa
     3001 tgggacgcga cgcaggctgg ctcaccgcat cgtcctgcgt actgcgtgca aacggcgagc
     3061 cggcaccgca ccttatatat cttcccgaga gcgatttctc ggaagagcgc tatcttgaag
     3121 atgttaaaaa cgcgtcaaag agatacaagg cggtcatagt ggcggtgtcg gagggaatag
     3181 actgccttgc aggtcggtcg gagcttacag acgcattcgg tcacaaatac ctgtcgggtg
     3241 tcggcaagta tcttgaggag cttacacgca gtaatatggg ctgtaaggtg cgttcgatcg
     3301 agcttaacgt aatgcagagg tgcgggtcac atatagcgtc gctcaccgac cttgacgagg
     3361 cgtggagaat aggcgcggcg gctgtgaacg aagcgctcaa atacggcggg agcggcgtaa
     3421 tgatgggctt taagcgtatc agtaacaacc cctaccgcgt gggtatcaca ccgcttgacg
     3481 taagggaatg tgcgaacaag gcgaagcatt tcccgaaaga gtggataacc ccggagggaa
     3541 acaatgttac tatagatgcg ctcaactact tcctgccgct gatacagggc gaaccgaaga
     3601 tagatttttt gaacggcata cctgtacatt tcagacttga ccagtgaaag ataaaacaca
     3661 atggggttgt gatacaaccc gaaaaaggaa aagtgagtcg aagcgcaaag atagtgcgct
     3721 cggctcactt tttgtatagt ttactcatat tatattgctt tagtaattaa caatgatagc
     3781 aggctaccga tagtgctttt cgcgggatag cac
//
LOCUS       DAWAOG010000153         3801 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3069_length_3801_cov_1.885211, whole genome shotgun sequence.
ACCESSION   DAWAOG010000153 DAWAOG010000000
VERSION     DAWAOG010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3069_length_3801_cov_1.885211"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    195..221
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1153 a          757 c          968 g          896 t
ORIGIN      
        1 aatctaaaca atgtgaacct cagcaacacc ggaataagca aggtagacct gacatcattc
       61 ggtattgata agtcagtcga ttattctaag ataaatcttg gttcggtaca gctgtacgag
      121 agtgatatag acaagtacgg acttgaaaat atgcttctga ctgaagtgct ttcacagaac
      181 ttaaaaattt ttttnnnnnn nnnnnnnnnn nnnnnnnnnn ntgctcaggt gagtgaggca
      241 gttcctgcac ttggtcttga aggtaagacg atcgatgata tcgacagcgg acttgtgaac
      301 aacgttgtag tgagcgttgt cgaatcgtca ggcaatcccg gtaaggcaat tcttgacaat
      361 gtggcgaagc ccgtgatact tgtgccgctg agaacagtga tatttgtgat actgttcttc
      421 cttgtattca taatcctgtc aattataata aaggcaacat cggtagtaaa caagcttccc
      481 gtgatcggta agcttaattc ccttctcggt ggtgtgctcg gacttgtaca gggtcttgta
      541 atagtgttta tcgttgtgat aatcgcgcat atggcggttg cgataagtaa taatacgctt
      601 atattcttaa acgaaatgac aataaacaag tcgtatgcat tcagctgggt atataacttc
      661 tcattcctcg atttccttaa ctaaatactc agttaacaaa agagaaatat aattataagt
      721 atgtggttaa aacagcttat actacagaca aaagaaccta aagaaaggtt ggatataaat
      781 gctttgttca agatgtaaaa aaagacaggc agttgtattt atctcgacga tacagggcaa
      841 cgaaaagcgc gatgagggac tttgccttat ctgcgcaaag gaactgggcg ttccgcaggt
      901 aaatgactac cttgaaaaaa tggggataag cgatgaggat ctcgaagaaa gctacaagat
      961 gcttttcggc gagggcgatc ccgacagcga agaaaacgat gaagatttca cacccggcgg
     1021 agcaggcacg atgatgccgt tcttccagcg ttttgccgca aataaggacg gaaagtccga
     1081 aagcgaaaag caggaggtca aggacgataa gaagcacaat aagaaaaaag aggagaagaa
     1141 gagaaaattt ctcgatgctt actgcactaa ccttaccgcc aaagccaaaa agggcgagat
     1201 tgaccgcata gtaggcagag agaaagagat atatcgtgtt atgcagatac tctcccgccg
     1261 tacaaagaat aacccttgta tcatcggtga acccggtgta ggtaagaccg caattgcgga
     1321 gggtatcgcg cagaagcttg caagcggaga tgtgccgttc agacttcaag gcaaggagct
     1381 gtatatgctc gaccttaccg cgcttgttgc cggtacacag ttcagaggtc agtttgaaag
     1441 ccgtgtaaag gcgcttatcg aggagataaa gaatgccgga aacataatcc tctttattga
     1501 tgaggttcat aaccttgtcg gaacaggtga ctcagaaggc actatgaacg ccgcaaatat
     1561 cttaaagcct gccctttcaa gaggcgaggt tcaggtgatc ggtgcgacta cattcaacga
     1621 gtacagaaag tatatcgaaa aggactcagc gcttgaaaga cgtttccagc ccgttactgc
     1681 aaacgagccg tcaattgagg atacaataga gcttctcaag ggtatcaagg aatattatga
     1741 agaatatcac aagctgtatg tgagcgatga tatattaaag agctgtgcag tgctcagtga
     1801 acgatatatt actgacagat atctgcctga caaggcgata gacctgcttg acgaagccgc
     1861 agcttgcgca agtataaaca gcaaggagct tacggaatac gaacagctca agaagaaaaa
     1921 caaggcgctt gaggtcgaag aaaacgacct ttcagccgaa accgagaata agaactatca
     1981 gcgtatagca gaaataaaga cagagcttgc aaagaactct gctcgtattg aagagcttga
     2041 gcccatgata aaagaggttc atgttaccga gaatgatgtc tgcaaggtga ttgagctgtg
     2101 gacgggtatc cccgcaagca agcttcttga aaccgagttc aagcgtatag caaaccttga
     2161 aagcgtactc aagtcaaagg tggtcggtca ggacgaagcc tgcgagcttg ttgctgccgc
     2221 tataaagagg acaagagtac agctgtctgc acgccgcaga cctgcatcgt tcatattcgt
     2281 aggacctaca ggtgtcggta agaccgagct tgtaaaggtg cttgcaaacg agctgttcaa
     2341 cacggttgac ccgctgataa gacttgatat gtccgagttt atggaaaagc acagcgtatc
     2401 aagaattatc ggttcgccgc cgggatatgt tggatatgat gaagcgggac agcttaccga
     2461 aaaggtacgc agaaagccct attcggtgat cctctttgat gagatcgaaa aggctcaccc
     2521 cgatgttatg aacatactat tacagatcct tgatgagggt aaggtaaacg atgcccacgg
     2581 cagaacggta agctttgaaa ataccgttat cgcaatgacc tcaaacgcgg gttcatcatt
     2641 caacacaagc ggacttggct ttgcaaagag cgaagaggat atctcaaagg ataaggctat
     2701 gaaggctctc ggcgagtttt tacgccccga gttcttaagc cgtgttgatg aggtagttgt
     2761 gttcaaaccg ttatcgcttg aagcgtataa tggtatagct caccttatga taggtgagat
     2821 gaaagagccg cttcttgaaa agaacatcat gcttaacgtg accgatgcgg catatgatct
     2881 tgttgcaaag aaagcgtcgg gcggtaagtt cggcggcaga gatgtccgca gaatagtccg
     2941 caaggatatt gaggacaagg tagctaatat cattgttgac gctgacggca acgtttcaca
     3001 gataaatgtt gacgcggacg gtgacgagat aaaggttaca ggcaaataat actgcggagc
     3061 attgcataat gcagtgctcc gtttttgctt aaacgaaagg aatcagattt atggcagtgg
     3121 ttattacggg cggcacagga gctatcggca gtgcgatggc acgcgagttt gcaaaaacaa
     3181 atgacgttgc gataatttac aaaaactccg atgaaaaagc gatacagctc gaaaagcaga
     3241 caggctgtaa ggcatataaa gcggacgtat cctctgtatc cgagatatcg gcggcggtac
     3301 gacagataat atctgacttc ggaaagataa atgttcttat aaacaatgcc ggcatatcgc
     3361 agataaagct cctctgcgat gtgactgagc ttgactggta caatatggtc gatgttcatc
     3421 tgacgggcgc tttcaatatg accaaggcag tactccccga tatgataaag cataaacggg
     3481 gaagtatcat caatatttcg tccgtttggg gggtatacgg agcgtcctgc gaagtgccgt
     3541 actcaacggt taaggcaggg cttatcggct ttacaaagtc gttgtcgaag gaggtcgcac
     3601 ctagcggtat aactgtaaac tgtattgctc cgggagtgat agacagccct atgaataacg
     3661 ctcatttgtc gcccgatgag atgaatgaac ttattgccga aacccctctc ggtaggctcg
     3721 gcacgcccga ggatgtcgca aaagccgcag tgtaccttgc atcggcgggc tttgtcacag
     3781 gtcaggtgct cggagtagac g
//
LOCUS       DAWAOG010000154         3784 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3089_length_3784_cov_2.149638, whole genome shotgun sequence.
ACCESSION   DAWAOG010000154 DAWAOG010000000
VERSION     DAWAOG010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3089_length_3784_cov_2.149638"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2052..2061
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3446..3455
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          906 a          914 c          946 g          998 t
ORIGIN      
        1 gcactgatat caccgtatga cagcggaaac ccttatgtca aaaagttgta ttgtgaaaag
       61 gacaaggctg cggattttta catcggcagt tgtcagctga cggcagacgg catagcggaa
      121 ataatgctga cagagtaccc ctctcttgtt atcccgcccg atgaatcccg gtggataagc
      181 gacatcaggt gtgacaatga cggaaatgtt ctgcggctca atgtctgcgg aattgaaatg
      241 tcgtgcgagc agttcagacg gctgttcggc atacgttccg ccgcatttga tatgagatat
      301 acacagcggc tgtacagctt tacaacaaag ggtgacggct gtaatacggg aatgagcgtg
      361 tattcggcgc ttaagctgtc agagcgcgga tacaccgcag agcagatact gaacgagttt
      421 tacgatttgt agaatatacg gaaatatata catataggat aacgcaagcg gtcgtgagta
      481 tgaatgcacg accgcttata agcatctaag atgctattgc attacttgct cctgccgaat
      541 tgtttttcaa acaacagaac ctcatttttt tgaatatatg ccgttatatc ggcacttata
      601 cgcgcattgc ttcaacacaa taactgtttt ctcaacccct ccagtatctt cttctccctg
      661 cgtgacacct gtacctgcgt cattccgagt atctcggcgg tgcggctctg ggtgcactcc
      721 tcgctgtagc gaagtattat aagcctgcgg tcaagcggct caaggcggga aacaagctgt
      781 cgcagagcca ttctgtcaag cgctttgggc tgttcgggct cgacaggcag gtcaaaagta
      841 ccctcgtcgt tatcctcgtc atcgtaattg ctgagggaga gcggcggcaa tgacgctccg
      901 agagccagcg acgcttcttc cggggttatc ccgagccgtt cggcaagcac agatatatgc
      961 ggctcttcac cgttttttat aagctcgtca cgcagtcggc agatcttcag cgacagctcc
     1021 tttagccctc tgctcacctt gatagttccg ccatcgcgga aaagctgttt tatctctccc
     1081 ataattaccg gcacggcata ggtggagaat ttaagcccgc ggctttcgtc aaagttgttt
     1141 cccgccttga caagtcccac acagcccgcg gaaaacagct cctcgtattc tatcccgcga
     1201 tccttgaagc ggctcgctat agagtgcaca agagcaagat gctcggttat aaaagagttg
     1261 tccctgtcgg tgctcatttc agctcctttt tgttaagcct gaaaatcagg gtgacggtcg
     1321 tgccctttcc gggctttgac gataccttca ccttgtcgca acagctctcc atcaccgcaa
     1381 agccaagtcc gctccgctca agctcgggct ttgtggtgaa aagcggttcc atagcctttt
     1441 tgatatcctc tatgccacag cctttatcgg ttatctttat gtatacctct tcatctttta
     1501 tacgagcagt tatatttata agacctaccg tttcactctc cgcataggcg tggacaacgc
     1561 aatttgtcac tgcctcggac accgccgcct ttactccgcc aagctcgtcc agtgtcgggt
     1621 caagctgtga aacaaaagcg ccgacagccg ccctggcgta gctctcgtta cgcgacaggg
     1681 cgggcacggt cagttttaca taattatcgc acttcatttt cattcctccg ttttcttgcc
     1741 gcttatgata agccttgaaa gtcccgctac ctgcaatatc ctgcggacgg acggtgacgg
     1801 attcttgacc gcgataccgc cgccgagcga ctccattata cgctgtctgc cgagtataag
     1861 tcctatccct gagctgtcca taaaggtcac ccccgacata tccagaataa gcatagtcgg
     1921 cataaggtgt tcaagctcag ggtctatctg catgcgtaca cctgccgcct cgcagtggtc
     1981 aagctcgccg cttatcacgg cggtgatgct tccgtttcgt tttatcattt caacactcgt
     2041 ataaagctcc cnnnnnnnnn ngctgtatgc tgacagcttg tattttatac cgcgtgaggt
     2101 gagaaacgtg tcggaacaag gaagagagat acgaatgaat attgaatatt gaataatgaa
     2161 taattgtggt ggcgctttct tgcggtcgtt tgtggtcgtt gcatcgtgca acgactttgt
     2221 gcgaccgctg tgaagcgtcc ttgcttcaag cgcggatttt gaattgagga tagtgcggaa
     2281 aaagaggttg tgctcttttt aaaatttaat gtgatattat aatttgctga aagttcagta
     2341 aagaactatc acacccggct cccttgtgta aagggagctg gcacagtgcg gtacgacatt
     2401 ttcggtattg cttctaaaat caggtcaaag atgtttagaa cagaaccgcg ttttcctgca
     2461 ctgtgactga gggattgtca gcggaggtga tagcaaaagc aaaaaggtca gtggcggcga
     2521 tccctcagtc ttggcggcag acagagcaag tgctatttga atgccgcggg tatcgctaag
     2581 gataacaaga tttagcgcac gcaagccggc tctatttagc gaggaagccg ggaattagtg
     2641 cttagcggca ttacatacct cccgactaaa atccgcattt caatttgaca acagcaagct
     2701 acatatgcta taatacactt aattgtataa acaaaatggg gcagaatacg caatgagcag
     2761 tttaccctga tataaagaca gaaaatacag gagtttttat gcagactaaa actttatgca
     2821 aattttcgcg cataccgcag acggtgtgcc gtatcgcctt atcggcggct atttttacag
     2881 gcatacttgt tgtgatggtt tttgcgcttg cgcttatagc aaacggaatg ataacactgc
     2941 cgtcggctgt gataagagtt gtcggcaagg gcagtatatt tgtgtccgac ctttcggcgc
     3001 ttacaatgct gttttttgga gcggcaagcc tgctgttcgg actgggactt atacttctga
     3061 gcacggcaag actgtgtccc gccgcggcat catatgtata cagacttttc tcgtccgtta
     3121 aagaatgaag ctgaaaaaca ttatccgctt ccgccatatg cgcgtgcaga aactgagcac
     3181 ggtccaggtg cttatccgcg tgttcttcgt gtcgggctcg gtgtgcctta tcgcggcgct
     3241 gtgttgcgcg atatttctcg ataaggacta cagaagctct gtaaagcgcg gctcacagag
     3301 ctttcctttt accgaatttc tgacggtgga aacgacagat ataccggtgt acataacgcg
     3361 gtctgccgat gatatgatac acgtttcgta tgtcagcgac accgaggtcg aggtgtttga
     3421 cgacggcggg cggcttgtga tagaannnnn nnnnnctgtt cacccgtgag cagttcagct
     3481 acaggataga aattcagctg ccggataagc cgtttgcaag ccttaacctc tattctgcgt
     3541 cgaccgacct gcactgctgt gcggtgagcg ggtatatggt gaatgtcagg tgcaagagcg
     3601 gcagtgcgga aattgagcgg ctgtcctgca cgggtacgct tgatatcgaa tgcgacagcg
     3661 gcaggataga tatgtatata gatgaatttt cgggcggtaa gcttgtaaac tcatcgggaa
     3721 gcatagacat ctactttgcc cgggacgcag atacggtgat ctctcccggt acacggtgct
     3781 atgt
//
LOCUS       DAWAOG010000155         3771 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3103_length_3771_cov_2.362756, whole genome shotgun sequence.
ACCESSION   DAWAOG010000155 DAWAOG010000000
VERSION     DAWAOG010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3103_length_3771_cov_2.362756"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1339..1348
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2140..2149
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2933..2942
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1138 a          720 c          874 g         1009 t
ORIGIN      
        1 actttgacaa tgtgcatttc agataccata agacaagcga ggaggaggtc ctcaccgata
       61 taaacctgca tataaagtcg ggtgaaacta tcggtataat cggcggtact ggctgtggca
      121 agaccagcct tgtaaatctt atcagccgtc tttatgacgt acagcaggga agtgtaaagg
      181 taggcggcgt tgatgtcaag gactacgata tgaagttcct gcgtgatcag gtatcggtag
      241 tacttcagaa gaatgtattg ttctcaggaa cgatccttga taacctccgc tggggtaacg
      301 aaaacgctac cgaggaagaa tgtattgagg cttgtaagtg ggcttgtgca gatgaattta
      361 tcgagcgttt ccccgataag tacaatacgg ttattgagcg tggcggcaca aatgtatcag
      421 gcggtcagaa gcagcgtata tgtatagcaa gggcacttct caaaaagccc gccgtactta
      481 tccttgacga ctctacaagt gcggtcgata cagctacaga cgctaagata agggcggctt
      541 ttgcaaaatg catacccggt actacaaaga taattattgc acagcgcgta tcaagcgttc
      601 aggatgccga ccgcatagtc gttcttgatg acggcagaat aaacggtgtc ggcactcatg
      661 atgagcttct tgcaaataac gaaatctaca gagatattta cgaatctcag acaaaggacg
      721 gcggcgactt tgaccgccct cagaattaag gaagggagcg taacgtaatg gataaaagaa
      781 aaatgggacc gcgtcccgat gtttcaccga aggaacaggt aaaactgctc ggaagaatta
      841 ttaaatacac attcagccat tataaattct catgtatctg cgttgttgta tgtattcttg
      901 tgcaggcagt aaccacactt gtcggtatgc tgttcgtaca gtcgcttatc gatgattata
      961 tcaagcctat gcttgaaaca ggcagtacag acttcacgcc tcttgcgtat gcaatgctca
     1021 gacttgcgat agtttacgtt gtcggtcttg cggcatcata cgcatataac aggataatgg
     1081 taaatgtagg acaaggtacg ctcagaaact tccgtaacga cctgttcacc aatatggaaa
     1141 agctcccgat aaagtatttc gacacacacc agcacggaga tataatgtcg gtttacacaa
     1201 atgatatcga tactttcaga cagtttatct gccagagtat tcctcaggta atcaactgcg
     1261 gtactactgt tataattaca ctcgtttcga tgatagtaat gagtatacct atgacgatac
     1321 ttgctgtggg catggcggnn nnnnnnnncg gctctgatgc ttgtcgtaac aaagaagctt
     1381 gccgcaaaat caggtaaata ttttggaaaa cagcagcatg atctgggcga ggtcgacggt
     1441 tatatagaag aaatgcttga cggtcagaag gtagtaaagg tgttctgcca cgagcaggag
     1501 gcaatagacg attttgtaaa gataaatgac aatctcagaa acagtgcgta taaagcaaat
     1561 aagtatgcga atattatgat gccgataaac gctaacatag gcaatatcag ttatgtttta
     1621 tgtgcgttac tgggtgcagt gcttgcaaca aacggcttcg gcggtcttac tctcggtact
     1681 attgtttcat tctgtacgct caataagaat ttcacaaacc cgatctcaca gctaagtcag
     1741 cagatgacat tcgtgataat ggcctccgca ggtgcaaagc gtctgtttga tcttatggat
     1801 cagaagcctg agcatgatga cggttatgta gagcttgtaa atgccgaaat tgatgaaaac
     1861 ggcaatatta cggaggtcga tcaccgaaca ggcaaatggg cgtggaaaca ccctcataag
     1921 gctgacggta cggtatcgta taaaaagcag gagggcggta tcgtctttga cggtgttgac
     1981 ttcggataca ctgacgaaaa aatggtgctt cacgacataa agctatatgc aaagccaggt
     2041 cagaagatcg catttgtagg ttcaaccgga gcaggaaaaa ctaccataac aaatcttata
     2101 aaccgcttct atgatattca ggacggtaag ataagatatn nnnnnnnnnc tggacgcaac
     2161 ggatgaagaa tgcatagctg ccgcaaagct tgcaaatgcc gatagcttta taagaagact
     2221 acccgatggc tatcagacta tgcttacagg tgacggcgct aatttaagcc agggtcagcg
     2281 tcagctgtta tctatagcaa gagcggcggt tgcagatccg cctgtgctta ttcttgatga
     2341 agcaacatcg tcgatagaca cgcgtacaga aaagctggta caggccggta tggatgctct
     2401 catgcacggc agaacaacgt tcgttatcgc tcacaggctt tcgaccgtgc gcaactccga
     2461 ttgtattatg gtacttgagc agggcagaat tatagagcgt ggtactcatg atgagcttat
     2521 cgctcaaaaa ggtaagtact acagccttta caccggcaac tttgcggaag aaacagcctg
     2581 agtgccgatt cggcacacca aatttgctgt tcctgaagta agtgatttgc gtactccttt
     2641 tgaaaaacag gtaatataag ttgaatacac aaaaaatccg ctgttttata acggcggatt
     2701 ttctgcttta ttgttttggc aaaaatcttg tttctgcttc tttggcttat ttgatattca
     2761 tttcgttaca agcttagaga tcatagtgaa attctgacct catcactaat ttgacattta
     2821 tccctttata ttatataata atattgagca ttttgacgaa agtcgtagcg ttatgtcgaa
     2881 attttgtcga aataacacga attgtgtcaa aatacgaata tatgataaaa ttnnnnnnnn
     2941 nngggcttgg gtgtttggaa tttcgtttta attttatcaa atcggacttt gtttcaaaga
     3001 aaatgaaaaa aattggtaaa atcaccttga attattttcc aggttatgtt atattataga
     3061 agcaataaaa ctatggaggt aagaaaatgt cacaatctat cagagtactt ataggggacg
     3121 attctttaga actgggtata tcgtgggcta cggctttcaa ggaagccgga atgtatgcaa
     3181 taacaagacc gaaatcagga aaggtgcttg ttgaatatgt gcttacggag cgaccggcag
     3241 ttgtcatact ggacgcaaaa actccgggtc ttgatgtatg cgatataatc aagacaataa
     3301 agaataatgt ggattatagc cctgtggtat ttgtaactgc aaattacgat tcggcaaaag
     3361 ttgaatttga ggtaatggac gcaggagcag actattatat gctccgtccg ttcgactcag
     3421 aaacacttgt gtcgaaagtg ctttccgttt ataatgataa gcttgcaggc aaacgtaaca
     3481 ctcttgctga tgagtcggat atagagtgcg ttgtaacgga gataatccat cagataggta
     3541 tacctgcaca tatcaaaggc tatcactatc tgcgtacagc aatcatactt tcagtcgatg
     3601 atcccgagat gattaactgt atcactaaat tgctttatcc gactgtagcg cgtatgtatg
     3661 atacaacacc gtcaagagta gaacgtgcta tccgtcatgc tattgaaata gcttgggaca
     3721 gaggagatgt agatacgctt aacgaatatt tcggttatac gatccacaca t
//
LOCUS       DAWAOG010000156         3764 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3112_length_3764_cov_2.607981, whole genome shotgun sequence.
ACCESSION   DAWAOG010000156 DAWAOG010000000
VERSION     DAWAOG010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3112_length_3764_cov_2.607981"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1353..1362
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2912..2921
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1073 a          720 c          935 g         1016 t
ORIGIN      
        1 ttttacatgg cagccgaatg cgatactatt aacgatacta ttaaaaggag cgattcgacc
       61 gatgaaaatc aaaaaccgtt ttaaaaaagc tgtggcggta ctgatgcttg tcacaatgac
      121 cgcaatttcc gtatgtgcct gtgccgataa gagcggatct gacaagtcag gcgagcctgg
      181 tgcggtgcag ggaaactact atcttgacgc agacaaatca aagcaataca tcagctttaa
      241 cgaggacaac agcttcagat ttgtcggata tgattttgaa gtgctcgcaa aagaccttgt
      301 cggtgatatg atagaagata agacggctct taccgaaagg cttgacgatg cttatacata
      361 tcattttgat aaggatacga atgagatttg gtttaacgtt ctgcagtccg atagctccga
      421 ttccgctgaa tttgtgattc cgatgcagct cggaggcgac aaacagagcg tgacgtttct
      481 gaataaaaca tataaaattg cacagtaaca attacggcac acttaatata cacggtgtgc
      541 cgtttttggt atcggaggga taacttatgg gcggtataat gtaccggacg gaaaatctaa
      601 ataaaagcta tgctgacggt gacggggtgt gctatgctct gagggatgta aatcttgata
      661 taagcaaggg tcagatggtg gcaattgtcg gcgagtcggg aagcggaaaa tcaacgcttt
      721 tgaacatact cggagcgata gacagcgcaa ccggcggaaa ggtcatagcg aacggaaaaa
      781 atatagcagg tctgtccgaa gaacagctta gcacttaccg cagtaaaact gtgggatttg
      841 tttttcaggc gtttcatatc attgattgct acacggtata tgacaatatc gagcttccgc
      901 ttataataaa caaagtgccg ggatcacagc gtgacgatat gatcaggaaa gcggtcgcac
      961 gggtggggct tgatgataag ctgagaacca agtgcaacaa gctgtcgggc ggagaaaagc
     1021 agagggtggc tgttgcaagg gcgcttgttc attcgcctga tgtaatactt gcggatgagc
     1081 cttgcggaaa tctggacagc cgtaacagtg aaagcataat ggaactgttg cgtgggcttg
     1141 ttgatgaaga tactcttgtg gtgatggtca ctcacagcaa gacagacgca cggttgtgcg
     1201 acagaataat tacgctcagt gacggtatgg tggtaagcga tgaaattata cgatgaaata
     1261 aggcttgctg tgagagaagg aaaaaagtgt ctgccttatc ttattattac cgcacttatg
     1321 tttgcgatag tttctgcggt tgcgtcctgt ctnnnnnnnn nnggattatt acgattatct
     1381 tgataaacag acgcagggag gacacgaatt cattgtaacc tgcggttata ctgccggtgt
     1441 taaggacaag ctgtcggagt gcggctttga taaagctgta catctgtcat cggcggatta
     1501 tgatgccggt atatatgtgt ctgacagcgg aaagaaaatc gggcttctgc aaaatcttcc
     1561 gctgtacttc attgatgagc tgcttagcga gtcctccggc ttcgactgtg aaatgatcga
     1621 cggagaggat ataaaaccgt gtttcgatac tccgggatct gacagtatgt ggataaatga
     1681 atatatagcc ctttcggaat cgcttgccgt cggtgataag ctgagtgtta aaagtaaaga
     1741 aaaaacgata aaggaataca cgatagcagg aatatacagc ggagagaaca cagtttttgc
     1801 gtatcccgtt atcgttccgt ttaattcata ttacgacagt gcggttgcaa ataaccgaca
     1861 gattgacagc gttttttcat gcacgataaa cagcatgcac caatatgaca aaacggtcac
     1921 ggcggcaagg caaaacgggt ttgatataga tacaagtgcg tttgaaatgg gtataaccgc
     1981 tattaactat gcgtacatac tttttatcgg gctggctgtt ttgtttgcag ggatagcttt
     2041 cttttctgtt ttaaatacat tttcggtaac aatagcgaaa cgctcgcata taatgacaaa
     2101 tttcatactg ttgggggcga gaaaaaacaa cgtatacgca atatatttta tcccttattt
     2161 ttcatcgctg ctgctcggcg ctgcaatcgg atttttgctg atgcacctgc tgttttccta
     2221 tgtttcatcc ctgacaaaaa gcctgctttg tttatagccg tacttgcggt tatgctttat
     2281 tgcaaattcc gcacgctgag cggaataaac ctctctgata cggtaagaga atatgacaga
     2341 taacagaaag gcgtaagatg aaaataaccg atatattaaa gctggcaatg aagaatctga
     2401 ccggacacaa gcgtattatg ctgagggtgg ctgtaagcgt tttgactgtt gtgctgctgt
     2461 gttgctctgc ggctgttttt gtgtcggcag ttaccgatga attatcggat atacagtaca
     2521 agcatataga tcaggcgtgt gcgatggtgt ctacatattc cgataccgca aagcaggcag
     2581 gggatactgc ggacgatatt atatcaaaaa taaccgccgt gtcagaagta atctcctgca
     2641 acgtcggtta tcgatacaat atatatacga caagcgaata tctgaattcc atatcacagt
     2701 caaattacag tgaagaccat ctggtgaagg atataacgct tatctgcggt gacgatatga
     2761 aaaaaactgc gttcgtggat atgatgaggc agcacaagca tattcttgcg gtagatatgc
     2821 ggtatgatgt gatttcggaa aatcaacgta tcagctttga acacaactat cccgacaaac
     2881 aaatcttttt gtacggcaga aacataagcg gnnnnnnnnn ngcgatgacg aggtcgttat
     2941 ttcggaagat tttctgtccg aactgggatt tacccgtgag gaaatggaaa ggctgacagg
     3001 caatcgtgtt tcactgaaac gtaccgacaa cggtgaaata tatcttgacg ggtttaccgt
     3061 ttgcggaata gcaagcagtg aggctgccgc aagcgtgttc gaacaaagct cgatgatgat
     3121 tacaaaaagc gccgccgaaa aaacaaaact ccgctacatc gatgccgaag tacatctgta
     3181 ttatgacagc tttcagaaag cgcttgaagc ttgtgagcaa gccgaaaaaa acggtattca
     3241 cgcaactgcc ggactcgatc ttatgatata ttcaagaata gacagagtaa gtgcctttat
     3301 cgaaaagatt ctgattgtta tactgccgct tatatgtgtt tcaatgctgc tgtcggcggc
     3361 atccgcactt ttgctgtatt attcggacac cgcacagaga atagctgtga tgagagcgct
     3421 cggagccaca aaaacggatg tgtacggaat aactcttgcg gaaacggttc ttgctgtttt
     3481 tgtttcgggt atagtcggat ttctgctgtc ggtcggtgta aatgccgtgt ataacatatg
     3541 gcttgaaagc tattttggtt tatctgtgca gattataagc gcagttcaac ttatcgcagg
     3601 tgcggtatcc tgtatgttta tgtttgcttg tgtacttaca gcgtcttttg taacaacgat
     3661 aaaaatctca tctgcatcta tagaccgtat gctgaaaggt gagcgttgac gccctcacgc
     3721 ctgcattgct cccttcggta tctgtctgtc acgaggccgc tttc
//
LOCUS       DAWAOG010000157         3754 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3121_length_3754_cov_1.998378, whole genome shotgun sequence.
ACCESSION   DAWAOG010000157 DAWAOG010000000
VERSION     DAWAOG010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3121_length_3754_cov_1.998378"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1086..1095
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2256..2265
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2913..2922
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          875 a          953 c          776 g         1120 t
ORIGIN      
        1 cacagcgtat cacctgttgt ggtgttctta acacctacag cagctgcaat atcgccgcag
       61 tagcaaactt cgaggtctgc tctgtggtta gcgtgcatct gaagaatacg tcccatacgt
      121 tccttcttgc ccttagttgc gttaagaaca gttgtaccgg ctgctacaac acctgagtaa
      181 actctgaaga agcagagctt accaacgaac gggtctgtag caatcttgaa agcgagtgca
      241 gagaacggct gatcgtcgcc tgcgtgtctt tcaacctctt cgcctgtctc ggggtcaacg
      301 cccttgatag aaggaacatc gagaggagag ggcatatagt caacgatagc atcgaggagc
      361 ttctgtacgc ccttattctt ataggatgta ccgcaggtta caggaacgat ctcattatca
      421 atcgtagcct ttctgagtac agccttgact tcttcaacgg tgatttcttc accctcgagg
      481 tacttgttca taagttcctc gtcctgctca gcaacagcct cgatgagtgc atcgtgatat
      541 tcctgagcct tctccttcat gtcgtccgga atttcttcca cacgcatgtc cttaccgaga
      601 tcgtcatagt agatgtcagc tttcatttcg ataaggtcta cgatacctct gaatgtatct
      661 tcggcaccga tagggagctg tatcggaaca gcattacact tcagtctgtc cttcatcatg
      721 ccgacaacat tatagaagtc agcacccatg atatccatct tgttgatgta tgccattctc
      781 ggaacatgat acttgtctgc ctgacgccat acagtttctg actggggctc aacaccgccc
      841 ttagcacata aaactgtaac agatccgtca agaactcgca acgaacgctc aacttctaca
      901 gtgaagtcaa cgtgtccggg agtatcgata atattgattc tgtgcttctt ccagtatgca
      961 gtagtagcag cagaagtaat tgtgatacct ctttcctgct cctgctccat ccagtccatc
     1021 gtagcagaac cgtcgtgggt ctcaccgatc ttgtgattga taccggtgta gaacaggata
     1081 cgctcnnnnn nnnnngagcc atgataccaa tattacgggt catttcaaga gttacgtctc
     1141 tagccatatt tcctccgttt tattgattac cacttgtaat gtgcgaaagc tctgttagct
     1201 tcagccattc tgtgtgtatc atcgcgcttc ttgcaagctc cgccctgacc gttcatagcg
     1261 tcgaggatct cgtttgcaag tctttccttc attgtctttt catttctctg tctggcgtac
     1321 agagtgatcc atctcagacc taaagttctt cttctttcgg gacgaacttc cataggaacc
     1381 tgatatgtgg caccgccgac acggcgtgcc tttacctcta actgaggcat gatattttcc
     1441 atagcttctt caaaagcttc aagtgcgctc ttacctgact tctctgctac gatgtcaaat
     1501 gcaccgtata cgatcttctg agcaacaccc ttcttgccgt cgagcatgat attgtttacg
     1561 agtcttgtta cgagctctga gccgtacata ggatcgggta atacatcacg cttaggaaca
     1621 ttacctcttc ttggcacttt acttccctcc ttcataaata atgaaaatca tcggtactcg
     1681 aaagcgatta actcccgccg tccaattgca gaggtaatat aatatattac tccacaaaag
     1741 tggctaaaag tcatcagtta cgcttttttc gcaccagcct tcggacgctt agctccgtac
     1801 tttgatctac cctgcattct tccgtcaaca ccctgagcat caagtgtgcc tcttacgatg
     1861 tggtaacgta caccggggag atccttaaca cggccgccac ggatcagtac aacgctgtgt
     1921 tcctgcagtt tgtgtccgat acccggaata taagcagtaa cttcataacc gttggaaagt
     1981 cttactctgg caatctttct catagcagag ttaggcttct taggggtagc agtacgaaca
     2041 gctgtgcata cacctctctt ctggggagaa gatatatcta ctgtttcctt cttaagagtg
     2101 ttcatgctct tctgcagtgc aggagccttg gacttctttt ctgcaactgc tctaccctta
     2161 cgaacgagct ggttaaaggt tggcaaatgt tacacctcct tgcaaaataa attaaattct
     2221 gtgcctcaga atgccgcatt tacgcattta ttctgnnnnn nnnnncacat ttaaatatta
     2281 tatacaaaaa ggcgacgatt gtcaagcatt ttcgttttca cgaaaaattt ttgtgataat
     2341 cgtcgccttt acgcttattt atcgcttagt tttgtgcagt tttgccaacc tgctcttctt
     2401 ccgaatgaga ttcttcagca tctgcatgat cgtatacatc tgcttccttt tctctttctt
     2461 ccttttctct ttcttccttt tctttttcca tagcctcaag acctgtacct gcaggtataa
     2521 gcttacctat gattacattt tccttaagac cgagtaacgg gtcaaccttg ccgtggatag
     2581 cggcatcggt aagaactcgt gtagtttcct ggaaggatgc ggcggacata aagctgtcgg
     2641 ttgcaagaga tgcctttgtg ataccaagaa gtaccgcttc gcactgaata ggtacgatct
     2701 gttcgccagc ttccttacgc tcctcaagct ccttattgat cttgacaagc tctgtcttgt
     2761 cgataaccga accgggaagc aaatagctgc tgccgggatc agttacacgt acctttctca
     2821 tcatctgacg aacgataacc tcgatatgct tatcgttgat atcaacaccc tgtaagcggt
     2881 aaaccttctg aacctcggca atgatgtagt tcnnnnnnnn nnatcatggg ggtttacgct
     2941 accttctgta agtgtttcgc cggcattgat ataatcgcct tcctggattt tgagcttaaa
     3001 tccgaacggt acgggataag gatgctcctc tccgtcctcg cttgtaatga taacatgtct
     3061 tgttttcttg atctcatcaa ttcttgtctt accgctgtgt ttagccataa tagcggcatt
     3121 cttaggacgg cgtgactcga aaagctcttc gacacgggga agaccctgtg tgatatcctc
     3181 ggcagatgct ataccacctg tatggaacgt tctcatcgta agctgtgtac cgggttcacc
     3241 gatcgactgg gcggctataa ttccgactgc ttcgcctatt gttatcttct tgccgtttgc
     3301 aagtgacgaa ccgtagcact tagcgcagac accgtgttca gcatgacagg taagaaccga
     3361 acgtatcttg actgtcttgg caccggttgc aacgatcagc ttggcatccg cttctgtcat
     3421 cagcttatcc ttggatacca gcacattgcc gttatcatca aggaagtctt ctgcaaggta
     3481 tctgcctgta agacgctctt caagcgactc aataagctcc ttgccttcgc gtatctcgta
     3541 gatatctatg ccgtctgtag ttccgcaatc atcggttctt actataactt cctgcgatac
     3601 atcaacaaga cgtcttgtaa ggtaacccga gtcggcggtt cttaaagccg tatcggcaag
     3661 tccctttctc gcacctcttg aggaaatgaa gtattcgagg atgttaagac cttcacggta
     3721 gttagctctg atcgggatct cgatcgtttc accc
//
LOCUS       DAWAOG010000158         3753 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3122_length_3753_cov_1.881828, whole genome shotgun sequence.
ACCESSION   DAWAOG010000158 DAWAOG010000000
VERSION     DAWAOG010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3122_length_3753_cov_1.881828"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    208..217
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1122..1131
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1479..1488
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2290..2299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          997 a          877 c          751 g         1088 t
ORIGIN      
        1 taaccgcgcc acagatataa tatctgcctt ctgcatattc atatctctgc ggatatcccg
       61 ttatcatatt gtttattatg atctcttttt tgatgccgag aaccgaatcg ataacacctg
      121 tcataccgac atcggtaata tatcctgtgc gaccgtcaag tatctcttca tctgctgtct
      181 ggacgtgtgt gtgcgtagcg agcacggnnn nnnnnnngct gaaaccttac cggtaagata
      241 atgtccgagc gcccttttct ccgaagtagc ttccgcatgg aagtctacaa ttattacctt
      301 tgatttgatt tcgctcagaa tcctgtcgat gcagtgaaaa ggattttcaa ggttctgcat
      361 aaaaacaaca cccataagat ttataacaca tatcgaatat ctgccgcgat caagtacagt
      421 atatccttta ccgactactt catccgggaa atttgccggt cggatcacta tgtccgatgt
      481 ttcgtagaag acatccattt cctttcttct gaagcaatgg tttcctgttg taacacaatc
      541 gatcccgctg tcaagaaggt attttgccgt tacaggcgtt atgccgtttc cgtctgcgga
      601 gttttcaccg tttgctataa cagtatcaat gttatattct cttttgagaa aaggcaattt
      661 ttctctgaga tttgcgcagg acttctgccc cacgacatct cctataaaaa ggatattcaa
      721 tcaaacacgt cctaaagctt tgttatcttt cgattatctg ttctctgtca ggacctacac
      781 ctaccattga aatatgacaa tcgcatagct cttccagtct ttcgatatac ttcctgcaag
      841 catcgggcag ttcgtcaaaa ctgcggcaag ctgaaacatc atcgtcaaaa cccttaagcg
      901 tttcgtatac cggtgtgcaa tctgcaagtt cttcaagcgc tgcagggaaa ttttcgataa
      961 cagtgccatc aggcttttta taagcaacgc agaccttgag gtcgccgatg ccaccgagtg
     1021 tatccagctt attgatagca agtgaagaaa gtccgtttac tctgactgag tgacgtacga
     1081 ttacagcgtc aaaccaaccc gttcttctgg gtctgcctgt annnnnnnnn ngtagtggtt
     1141 ccgaactcgc cgcccttgtt tcttatggtt tcgcctatct catcatcaag ctctgtcggg
     1201 aaaggacctt tgccgactct tgtggtataa gcctttgcaa cacctatgat gttagatatt
     1261 atcataggac caacgcctgt accgacacaa acacctgcag ataaggggtg tgatgatgta
     1321 acataaggat aagtaccgaa gtcaatatca aggagcgtag cctgtgcgcc ctcaaacatt
     1381 atcgtcttgt ttgccttagc ggcttcataa acgataaccg atacatcatc tacatacttt
     1441 gccagacgct taccgtattc ggtgtattcc tttatgatnn nnnnnnnngt catgaggttc
     1501 gccgccatac acttctgtga ttatcttgtt cttgagcttg cctgtcattc ttgccttttc
     1561 agcaaaaacc tcagggtgaa caagatcgta aacacggata ccgcaacgtt catatttatc
     1621 catatagcaa ggacctatgc cgcgctttgt agtacctata tcggaattgc ctctgaactt
     1681 ttctgaaagt ccgtcgagcg ttatatgcca gggcattacg acatgagctc tgggatctat
     1741 cttcagatta tcaaaagaaa cgcctctgga tgaaaggttg tcgatttcgg ataaaagatc
     1801 cttaggatca agaacaacac ctgcgccgat aaggcagggt gtgtttttat agagaatgcc
     1861 cgaaggaata aggtgaagct tgtaagtctg accgtcattc actacagtgt gacctgcgtt
     1921 gttaccgcct tgtgaacgca ctacaacatc agctcttgat gcgataatat caattatctt
     1981 gcccttacct tcgtcacccc attgagttcc tacaactata tttgccggca ttgaaagtcc
     2041 ccatttctgt ttaaaaaaat attatatttg caacgggcgc gacagccctt tgcatagcac
     2101 attaacatta tagcaaaaga gagcgaaaat tgcaagtctt ttttgtttct ttcgcaaaaa
     2161 aaggctctgc atatgcacaa atgcggcaag catatgacct gccgcatttg caattatcag
     2221 taagaagaaa caccgcttct tgttacatat tcctgtattc cgtcaacaat agcctgtgca
     2281 atgccgtctn nnnnnnnnnt tgatcggtag aatttgcaag cgccatagca tcttcatagt
     2341 tgcttacaaa tcccatctct ataagcacag acgggaaatc ctgctgcttt gtcgtccaca
     2401 tatagctgta ctgagcacct ctgttacaat cagctccgtc agaataaaca ctgttgctga
     2461 aataagatga aacgtgtctt gtaagcgact cggcaagcgg ctgagaatat gaagtatagt
     2521 aatagcattc tgtgcctctg gcatcttccg agcctgcctt gttggaatgg atagctatat
     2581 aaaggtcgca gccgtaatct cgcgcataat acggtcttct catcgtatca taaaattcgg
     2641 actcggtatt gagtcttaca acatttacgc caagctcttt gagtttggct tcaacaagct
     2701 ttgcgaccgc aaggttagca cccgcttctt cgatatgacc tatagcgccg gggtcgtcat
     2761 agccgtattc cgatacaccg tgtcccggat cgattactat agtcatattt gtgatatcgt
     2821 ttgtgagtat ctcaaacttg aacataagat ttccgtcaga gtcatatgta gcgctgtttc
     2881 ctgcgtatac accgggctga cggagcttca gcacaagcct gaatttcata acgccgtcaa
     2941 cttccacctg ctgccattcg ccggcactga atacagtaca gttatcaaaa ttgggaagtg
     3001 atgtgaccga agttacatta tcaaaagtaa cataaatata ttgagcggta aaatcgtcca
     3061 aattgaaatc tccgtctaca ccggtataat attcattgcc gataggagtc accgtgaaag
     3121 aagaacggtc ttcaagcgac atctgtataa atgaatcgcc gcccgagttg cctattgcct
     3181 ttacaacaag cggattttcg cccattccga caccgctcac aaggtcagcg tcctccttaa
     3241 ggaaacgctt gcccgaggtg gtcacataat aatcgtccca ttcgctttta taatagtcga
     3301 gtgttccctc gggaagctgt ccgacattcg gaggattaac agaatctgta ttatttccgt
     3361 cgtaaatata agcaaaattc ttattcagct taacataagt aacatctgtg tcttccgaaa
     3421 ttgcaggcga gagctttccg acaacttcgg aatcgccgaa cgaactctga tccgcaggta
     3481 tttcatcgat aatttcaacg tttttctcag gaagctttac agcgtttact gttacctcag
     3541 cgccaatata tgttcgtgaa tatcctgcat aagaggctga tatttcaata tttccaaggt
     3601 actgctcttc atcgacaatt ccctcgggaa cggtaatctt acctgtaaat gcagcatatg
     3661 aggagttgat atcaactaca tcggatttag cgttttcggt aagctcaata gtagtaccgc
     3721 cgagagtagc ggtagcgtat gagcctttat agg
//
LOCUS       DAWAOG010000159         3750 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3124_length_3750_cov_1.757240, whole genome shotgun sequence.
ACCESSION   DAWAOG010000159 DAWAOG010000000
VERSION     DAWAOG010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3124_length_3750_cov_1.757240"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1668..1677
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2417..2426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1002 a          840 c          898 g          990 t
ORIGIN      
        1 ataatgaaca cgtcggcgag gagttttgcc gctacagaca ggaaatatcg acattgctcg
       61 ttgaagaaaa caaccttatg gagattgtaa agcttatcgg tgcggatgtt ctgccggacg
      121 accagaagct gataatagaa atcgcccgtg ttataagagt aggcttctta cagcagaatg
      181 cttttcataa ggacgatacg ttcgttccgc tggaaaagca gaagctgatg atgaaagcaa
      241 ttttacttct ttatcataac tcgctgaatg ccataaacgg cggcaagccc atatcggaga
      301 ttatctccta tggcttcttc gataagctgg taaagatgaa gtatgatata cctaacgaca
      361 accttgaaat gtttaatgaa tattttgata tgataaacag caggtttgcc gaagaataaa
      421 cccgatagga gaaagtttta tgattcaata tgcaggactg agtgaaatta acggaccgct
      481 tgtctgcctt gagggtgcta agggtgtttc ttacgatgag atcgcagaga taaagcttga
      541 ggacggcaca aagcgtttag gtcgtgtcgt tgagatgcag ggcgataaag tagtcttaca
      601 ggtatttgag ggtactaatg gtatctctct taccaataca aaaatcagtt ttacgggaaa
      661 gccgcttgag ataccgctgt ctaccgaaat gctcggcaga acgttcaacg gtgcaggcaa
      721 gcctatagat ggactgggcg aggtgttccc gcaaaaattc ggcgatataa acggacgtgc
      781 gttaaatccc gttgcacgtt catatcccag gaactatata cacacaggca tttcctcgat
      841 agatgctctt atgacgctta taagaggtca gaagcttcct atattctctg gctcgggtct
      901 gtcgcacaac aagcttgccg tgcagatagt aaagcaggct aagattgctg acagcgaggg
      961 caaggacttt gcggttgtat tcgccgcaat gggcgttcag aatgacgttg cagagtactt
     1021 cagacgttcg tttgaggaaa caggagttat acagcgtgtt tgtatgttcc ttaatctctc
     1081 aaatgatcct attatagagc gtatccttac cccgagatgt gcgcttactg tggcggaata
     1141 tcttgcgttt gagaagaata tgcacatact tgttatcatg accgacctca catcttactg
     1201 tgaggcactg cgtgagttct cttcgtcaaa gggtgagatc cccggaagaa agggctatcc
     1261 cggttatcta tattccgacc ttgcctctat gtatgaaaga gcgggtgtag tagagggcag
     1321 tacaggctct gttacacaga tacctatact tacaatgcct aacgatgata ttacccatcc
     1381 tatccccgac cttacggcat atatcactga gggtcagata gtattcgaca gaaacctcga
     1441 ccagacaggc gtttatcccg gactttctgt actgctttcg ctgtcgcgtc ttatgaagga
     1501 cggtataggc gagcagtata ccagagccga tcacgccgat gtttcaaacc agcttttcgc
     1561 ttgctatgcg agagttcagg acgcaagagc gctcgcatcg gttatcggtg aagatgagct
     1621 ttcggcatcg gataaggcaa ttatgaaatt cggtacgatg tttgaaannn nnnnnnntcc
     1681 ttaatcaggg atttgacgaa aaccgttcga tggacgaaac gcttgacctt gcgtgggatc
     1741 tgctttcact gcttccggca agcgaactcg accgtgtaaa tgagcagctc atcaaggagc
     1801 attataaccc tgaaaatgcg aagagatttg tataacagtt actttatgaa aggcaggtga
     1861 gtgtatggct gaacaggttt tcccgaccaa aggcaacctt atcaaggcaa agcgttcgtt
     1921 gcagcaggca aagcagggct ttgagctgat ggacagaaaa cgtaacgtat tgatacgaga
     1981 aatggtcgct ctgaccgata agtcaaagct tgtccgcgga aatatggcgc agtttgttcc
     2041 tgaggaaacc gatcttaaga tatcgttcag aagcgttatg ggcgttgagc ttcctacagt
     2101 cgagcttccc gataagccta tagttccgca gtatgacatt gcaatgacaa attcgtatct
     2161 tgataacgct tatatctgct tcaacgaaac aaaaaagctg acagtcatac tcgccgagat
     2221 agaaaacagc atataccggc ttgctgtggc tattaaaaag acacagaagc gcgccaatgc
     2281 gcttaaaaac atcattattc cgaaatatga ggcgcaggtc agatttatct ccaactctct
     2341 tgaagaaaag gaacgtgagg aattttcacg ccagaaggtg atcaagagta ccaagataaa
     2401 gaaggaacag gtgcagnnnn nnnnnnagcg aaaacagaat aaattaaagc cacacagaaa
     2461 gttgatccaa tctgtgtggc tttttttatg tcggtatcac ttaaacttca acgaagatcg
     2521 ctccgcttgc gggaacagtt actttatcgc cgcttcccga gccgttttcg ccgagcgcgt
     2581 aaacagcctt tccgcccttt gcataatccg cattgacggt tacagcctta tcagacggat
     2641 tgatgaatac atatactgtc tgagcgtcac agcttctctt gtatacgaga ggatagccgt
     2701 cactgctgac aaactcgaat gaggaaaggt tatggagtgc gggattgtcg tttcttacct
     2761 tgataagctt ctttatttca ttatacagcg aattactgtc aagcatctgc ttttcaactg
     2821 tgggtctgtc gggggcatcg tcctgcttta tataaagctg tgacttgtcg gcagttgaaa
     2881 agccgtcatt tgctgtgctg tcccactgca taggacttct tgaacctgta cggttgtagc
     2941 cgccctcgac cgatacaagt ccctcgacat atcggagtcc tatctcatcg ccatagtaca
     3001 cgaatggcgc gccgggcatt gtcagcagga aagcaaagca gcatttcagc tcgtcctcat
     3061 ctcttcccct tgcaagtctg tccatatcgt ggttacctga cggtatacag ataagtccgt
     3121 cgcctgttgt tgccttttcg tgatagccat tgtactgctc ggcaaagatt gcgggagtac
     3181 ccttgcctct ctttgagaag aaaggctcgg cacatctgaa cattgagttg tacccctcag
     3241 gaccgaagtg cagtaagaaa tccatatcaa agccgccctt aagcgaatac tgcggctcgc
     3301 cccactccga taccattgcg gcttcaggat attcactctt aagatatccc gtgatatcct
     3361 gccagagctt tatagtttcc ttatactcgg gatcgcactt tacaagcgat cctgccatat
     3421 ccactctgaa gccgtcacag cccatgtcga gccagaactt cataacgtcc tttatagcgg
     3481 ctcttgttgc cataggaccg ggatctgtgg gcttctgctg gtacgaatat ttaggctcgg
     3541 cgtagccgta gttaagagcg ggctgagaag agaagaagtt gacggcggcg cttccgtcac
     3601 ggtcagagat acctcttatg cagttgagtc cctcgggctg ttcccatata ttgctcgtcc
     3661 atatatatct gtcggtgtat tcgttctttt cgggtagcat tgactgcttg aaccattcat
     3721 gctctaccga agtatgaccc ggtacaaggt
//
LOCUS       DAWAOG010000160         3741 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3135_length_3741_cov_2.171188, whole genome shotgun sequence.
ACCESSION   DAWAOG010000160 DAWAOG010000000
VERSION     DAWAOG010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3135_length_3741_cov_2.171188"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3360..3458
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1212 a          777 c          874 g          779 t
ORIGIN      
        1 gttcaggagg aactgatgcg gaagacaggg atgacactta cagaaaccta taacggctac
       61 ggtgctaaaa taaaacacac atcagcccct aagggtttta taggtaacat ataatttcgg
      121 aggataaatg agagcttcat ttttaggatt agagatccag aaaagatcgg tacagatggc
      181 gcagaaggcg ctcgatgtga ccggaaataa cctgtctaac cgtgacacgg taggctatac
      241 aagacagcgc cttgacgtat attccagcta tatgaatatg aacgggatat acaccacaaa
      301 gctcgcccgt ctgggacttt cgagccaggg cgtatttgca agcggcgtaa gtcagaccag
      361 agatgattat cttgacaagc gttacaggga caacagctgc tatgttgccg agtataacca
      421 gcaggcatct ataatgagcg agatcgaaac tgcgctgacg aacttctccg atacatatga
      481 gaatgttggt atggcatatc agttcgacca gttcaaatcc gctctgcgta aatatgcgga
      541 ggacagtgcc gacagagagg agcttgcaag cgttgtaaga aaccaggcat acaatatatg
      601 caagctgttc actcagcaga gtatggatct taaaaacctt gaaaatcaga ccttatacga
      661 tctgcactcc acgctcgaag atgcaaaccg tataatagac gaaatagtcg aatacaacaa
      721 gcagatagta aacgagtatg cgataacagg agcagacctt atatataacg gcttatccgt
      781 tacaggcggc tacggaccta acgaaatgct tgatgcaaga aacaacctta tcgaccagct
      841 cagcgagttc ggcgacattc atgttgaaga taactttgac ggttcggtaa agatcacaat
      901 gggcggtctg atgatcgttg acggtcagaa gtgcaaccgt tttgtaacag gtaaggattt
      961 tgaagcgtac agcgctaagt atgaagaaaa cggtgcggcg gtgctgaagc tgacagatgg
     1021 caacaatatg gtgcttgaaa gcggctctat aaaggcttat accgatatga taaacggcaa
     1081 cggcgcatat gcatctggca agcagaccac cgactacggc attaaatatt atcaatcggc
     1141 tgtagacgaa tttgcaaagc aatttgcagg gcttatgaac aagctcaacg gcggcgatga
     1201 atcggacgac aggcttatgt tcacaagtgc cgacggctca ccgataaccg cagggaatat
     1261 aagaatatca gacgcttggc tgaaggacgc tacgatgata gcgaagatat acaacgaaaa
     1321 gacaggcgct tatgattacc ccgtaaatct tgacggtaat gcggtaaaca agctactgct
     1381 cggaatggac gacagtgttc agataggcaa gggcgactat gaaggctcaa gctacgactt
     1441 tatactcttc ctcaacaacc gtcttgctca gaatatcgaa tttgcggaaa agcagtgcga
     1501 tatgtacacc gaaacatcga cctcgcttct tgacagcaga gatgcgataa tgggcgtttc
     1561 cgaaacagaa gagggcgtta atatgctgaa ctattccaag tggttcaacg cttcctcaag
     1621 aatgctgaca acgcttgatg aggcacttga tactataata gaaaaaatgg gacttgtcgg
     1681 cagataagcg gaaaggataa tttgatatga gaataacaca gagcatgacc aacagacgct
     1741 atatgtcgca gcttaatgcc gctctcgaaa gaaaaaacgc ctccgagcga aagatacatt
     1801 cgaccaagag atacaacaga gcaagcgaag acccgataag cgccgcaaaa gcactgagaa
     1861 caagaaaagc gatctcaaac accaacgatt atctgggcaa ccttgaaacc gccgaacaaa
     1921 tatataacgg tgcagacagt gttctgatga atgtaaacga cattctggac aacataatcg
     1981 agaaagtaac atatgccgca aacggcactc agcatgatga agatgaggag atcctcgctc
     2041 agactgtcga aactttcgca gatgagatag taagactttt caacaccgat atagccgaaa
     2101 gaagaatttt cggcggtgta aacaatgact tcaccatatt caagatcgaa aatgccggcg
     2161 gcaaaaagac gattacctac aacggagttg atataaactc cattgacgat ccggacaaat
     2221 tcccctattc agaatcaagc tatacggaca tcggtatcgg aatggtgatc gaccccgcaa
     2281 caggtagagt agatcaccag tcggcactgc ccgtaacatt caacggtgca aagataacag
     2341 gctgcggcaa ggatgatgaa ggcgactcca agaatataat ccagataacc cttgatgccg
     2401 cacaggctgt aagagagggc gacaagataa aggctatgga ctatatcgac aagctgagag
     2461 cggctcagac aagcgtcagc gtcgctcacg ccgatatagg aaataagcag gagtatatcg
     2521 aatacaataa aaatcgcctt acaaacaata tggaaactct gcttgaacag cagaacaacc
     2581 tagaaggcac ggatatgggc gcggaaacga ccaactggaa aacgcttgaa gcgatctaca
     2641 acgtttcatt gcagtttgca tcatctgtaa taccgatgag catatttcag tttatttcat
     2701 aaattatcgc tctgagtgaa agagtgaaaa ataatacggg gaggatgata ctatgaatcc
     2761 gaatttgctt aagatcacaa gcataccgat aagcattgag gtgaatgtca caaacggtac
     2821 attaaaagca cctgaggaca aagagccgct caaaatcgat atcaaccgca cacccggcgg
     2881 atacacgatg aagagcagac ccgcaaagat aaatatcgat acttatgcgg cacgttcaag
     2941 tatgggctat ggcgaataca atgccgccga cttcaatcag aaggaagctg acagaggctt
     3001 taagatagcc tatcagggag tagcaagaat agtgagcgag ggcgaccagc tcgcaagagg
     3061 tgtatcaccg tcagagattg ccgcacagca gatgcgtgcg ggcgcaacca tagaaacggc
     3121 tatagatttt ctgcctaagg gcggagctga tgtatccttt gataagggta cgctcagcat
     3181 aaactatact cccaccgata taaatatcga ctgggaaaac ataaattcct ccgttgtcga
     3241 gtttgttccc ggcaatatag agtttatagt aaaagaacgc cccagagtgg aaatagaata
     3301 tgtgggcgag cctatatacg ttccgccgag tgcaaatccg gcatacgttc cgcctaaaan
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gtaaagatca atggagataa
     3481 tgacaagaga tttcggtaaa gtaaatgtag acgaaagcaa gatattcaac ttcccgtcag
     3541 gactttttgc gtttgaagaa tgcaggcagt ttgctttgct cagtccgctc ggcgaaaatg
     3601 tataccctat gtggctgcag tctaccgaaa atgttacacc gtgttttatc gttttcgacc
     3661 cggctattat agacggagct tataagataa cgcttaacag ctctgaaaag aaactgctga
     3721 agataaacga caactcaaac g
//
LOCUS       DAWAOG010000161         3718 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3155_length_3718_cov_2.633361, whole genome shotgun sequence.
ACCESSION   DAWAOG010000161 DAWAOG010000000
VERSION     DAWAOG010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3155_length_3718_cov_2.633361"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2954..2963
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1081 a          813 c          934 g          880 t
ORIGIN      
        1 ggcttgagct tagctccata tcaaatataa taagctgtgc gtcaagatcc tttgcggcgg
       61 cactgagttc ttcgatcttg ccctcgccca gaattgtcgc actttcgata gcaggacgtt
      121 tctgaataat acggcataca gtcaccgcac ctgcggtttc agccagttct tcaagctcgt
      181 ccatagatat ttccgcatca tattcgccgg tatctactcc tgcgagtatg gcacgggtcg
      241 attcgttttc ctttaagatc ggattgttta cggttgattc gttcataatg ttcctttccg
      301 gtttataaat aatcaataat aaagtaaatt tatgcggtaa tccgcaaaag gtggtaatct
      361 ttatgaagtt caaagaatac gacgtaataa ttgtcggcac aggcgtttca ggtctttatg
      421 ccgcactcaa ccttgacagc agtatgcaga tacttatgct ctcaaagcgt gagctgacgc
      481 tgtgcaactc ggctctcgca cagggcggtg tcgccgctgt aatggacacg tcaaacgaca
      541 actacgagct gcacatcaag gacactctta tagccggcgg ctacaagaac aatatcgaca
      601 atgtacgcat actcgttgag caaggtccga aggacgttaa aaatctgctc aattacggag
      661 ttgactttga cagaaagcag gacggctcga tcgaccttac acttgagggc ggacactgcc
      721 gtcacagaat cgctcatcac aaggacagca caggctttga gatcgttact tcgcttatcg
      781 agggagtaaa gaagctgcct aatgtcacaa ttcttgaaaa ctcacacctg ctcggtctta
      841 ccaagcgcgg tgacaatttc ctgtttgatg tgcttcacga aggaaagcac agctattata
      901 ccacaaagaa caccgttctt gcaacaggcg gtataggaag agtttacgat tataccacaa
      961 actcggcgat tgctaccggt gacggcatac agtttgcata caatatgggc gctgatattc
     1021 agcatctgag ctatgtgcag ttccacccca cagcctttgc cgaccacgaa aaccgtgagt
     1081 gcttccttgt atccgaggcg gtacgaggcg agggcgcata tctgctcaac tgcaacaagg
     1141 aacgttttat gcataagtat gagcctgagc gtaaggagct tgctccccgt gacgtggttt
     1201 ccaaggatat gatgaaagag cagaaagaaa cgggcagcga caagttctat cttgatattt
     1261 cgtataaaga tcccgaattt atcaaaaacc gcttccctat gatatatagc aaggttcttg
     1321 aaaagggcta tgatatgaca aaagagccta tcccgatata tccttgtcag cactacctta
     1381 tgggcggtat aggcgtaaac ggacacggcg caacaaatgt caaaggctta tacgccgcag
     1441 gcgaatgtgc gcataccggc gttcacggta tcaaccgtct tgcaagcaac tcactgcttg
     1501 aggcactggt attcagccgt cttattgccg aggatatcaa taagaacaat gttcatcacg
     1561 agctcggaac ggttgaattt gattatccgt cacccgatgg caagccgctc cctaagggaa
     1621 tacgcaccga gataagacac ataatgcagc gctcttattt cgttgtgccg gactatgtag
     1681 aagcggctaa ggatataaag cgcgtaacgg agcttaaaga cctgcttgat aacggcggtt
     1741 tcgaggtaac tcccgactat attgaagcta agtcgctgac taccgttgct tacataatcc
     1801 tcagcgaagt ggttgcagag ggaaaggaaa agggcattat ataatgaaat tattacagtt
     1861 ttacgttgac gatctgataa aaagagcgat aacagaggat ataaactaca tagacagcac
     1921 taccgacctg cttattgacg aggacgatat atccgaagcc tattttgtat caaaggctga
     1981 cggcgtgctt gcgggtattg atgttgcaat gcgtgtgttc acattacttg acgacaccat
     2041 cgagatgaat attcatttca aggacggcga cgctataaag aaaggcgatg ttatcgccga
     2101 gttcaagggt cacacctgcg ctatgctgaa agccgagcgt acagcgctca atcttttaca
     2161 gcatatgagc ggtattgcaa gctatacaaa caagtgcgta aaggcagtcg agggtactga
     2221 tgcttctatc gcagatacga gaaaaacgct ccccggctta cgtcctttgc agaaatactc
     2281 agtagtggca ggcggcggaa gaaaccaccg ctacaatctt acagacgcgg ctatgctcaa
     2341 ggacaaccac attgacgcgt atggcggtat tacacaggct gttaccgctc tgcgtaaaaa
     2401 agcgggacat atgcttcaga ttgaggtcga agtgcgcaac tttgacgagc tgaacgaggc
     2461 gcttgcggta aaggctgacg ttataatgct tgacaatatg agctgtgagg atatgaagaa
     2521 agcggtagag ataaacgcag gcagagcaaa gcttgaagca agcggaaacg taacgcttga
     2581 caatatccgt gacgttgcgc tgacaggtgt tgatataatc agccttggcg cgcttacaca
     2641 cagtgtaacg gcttttgata tttcgatgaa gtggaagaag aaagcatgac aaactgcgac
     2701 tgctgcgtaa actatgtcta tgacgatgag tacgactgct acacctgtct tgtaaacctt
     2761 gacgaggacg aaatgcgccg atttataaca ggcgacaatt acaactgtcc gtatttcaga
     2821 aacgacgacg aatatgagct tgcaagaaag cagtaagctg aaaggaaaag ctatgacaaa
     2881 gataccgagg ggtgacaaat catatatcgg aaagactgtc actgtgacgg ttgaccgacc
     2941 gataggcaca catnnnnnnn nnngcacaca tcatcccaag cacccggaga tagtatatcc
     3001 gataaactac ggctatgtcg aggggcttct cggcggtgac ggcgaggagc aggacgtata
     3061 tattctcggt gttgataagt ccgttgacaa ggcagagtgc gttatcatcg caacgataat
     3121 gcgcaccgat gacaacgagg acaagtgggt aggtgttccg gctgagcttg tcggcacgga
     3181 gctttgctgt gagtgcaata tagtgaacgc cgtgcatttt caggagcaat tccacaccgc
     3241 tgagatattt gccttatatg aaaagacctg cggtgcggta atgtacacag gcgacaaggg
     3301 taaccgcaga tacttcctta tcaagaataa cagcggtcat atcggctttc cgaagggtca
     3361 tatcgagtac ggcgaaagtg agcgtgaaac agctctgcgt gaggttttcg aggaatgcgg
     3421 acttaaagcg gaattagatg aaaatttccg tgcggagtat acattccaca cgcttgataa
     3481 caccgaaaag acaagcgtat tctttgcagg aagatttgac agggatgccg ccgtgaagat
     3541 tcagcaggaa gaggtaatag gcaactggct gttaagctat gaggacgctg tgaacaagct
     3601 gaactgggaa caggataagg gtatactgaa agcctgtgag gagtacctga acaggaagag
     3661 taagtaagat aagtatatca aataaccgca gtatcggata gatactgcgg tttactgt
//
LOCUS       DAWAOG010000162         3704 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3166_length_3704_cov_1.975062, whole genome shotgun sequence.
ACCESSION   DAWAOG010000162 DAWAOG010000000
VERSION     DAWAOG010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3166_length_3704_cov_1.975062"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1233..1250
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2501..2510
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1109 a          718 c          927 g          922 t
ORIGIN      
        1 tttgctgtta ttgctgatta aatttgctct gtctgaagtg tcaacgcaat ttaaagacat
       61 aacgacggcg aatgtcaata ataatgcacc gcctatgcct atgagtgtat cggtcgattt
      121 tcccaagatt ttttactcct ttttttgttg aatatgcatt ttctttattt gtagttataa
      181 agtctgtata ctattatatc aaggagtggc aaaatagtca acagctatat cgtataagat
      241 ttacttaaaa atgggcgttc ggcgttggcg gctctgtcgg ttttgtatct ctgcgaggct
      301 gtatacaagc tgtcggacaa ggtcgatacg ggacgcaact caaaatacga tcacagttgt
      361 gccaaggcaa aaccatcaga gtgtcataaa cgggacagga ataacgtaca ggggcttttc
      421 agaaaagcaa gcttttttgc aagctgcatg gaggtaattc tttttatgct tcaaataaaa
      481 gagttatagc cgttttttct tgacagccgc atgaaatcat gatatactta aaaacgtata
      541 aatcgaacaa aagaaaacgg aggatagttt tcaatggcaa aaatagcagt tctcggctat
      601 ggtgtcgtag gttcgggcac agtcgaggta attgagaaga ataaggcggg tcttttaaag
      661 aaagccggtc aggagataga gataaagtat atcctggatc tgcgtgattt tcccgacagc
      721 ccttataaag acaaatttgt aaaggatttc aatattattc tgaacgaccc cgaaatttcg
      781 gtagttgttg aggttatcgg cggaataaag ccgtcatatg actttgtaaa gtcttcgctt
      841 cttgcaggca agagcgttgt tacatcaaac aaggagcttg ttgcaaagaa gggcgcagag
      901 cttctgaaga tagcacatga gaagggtgta aacttcttct ttgaggcgag cgtaggcggc
      961 ggtatcccga tcataagacc gctccaccag tgcctttccg ctaaccgcat agatgaaata
     1021 gcaggtatct taaacggtac tacaaacttc atactcacga agatgatacg cgataatatg
     1081 gcgtttgatg atgcgttaaa gacggctcag gagcttggct atgcggagcg taatccttct
     1141 gccgatgttg atggtatcga tgcttgcaga aagatctgca ttcttgcttc tctctcattc
     1201 ggaaagcacg tttatcccga taatatccat acnnnnnnnn nnnnnnnnnn tcgccgttat
     1261 ggtatgcccc gcatttatcc atggggacag ccagctcgca ggcgttaacg atgtattcaa
     1321 cgcgatcctt gtacgaggtg actctacagg tgacgttgta ttctacggta agggtgccgg
     1381 taagctcccg acagcaagtg cggtcgtagc cgatgttgtg atagcagtga agatgaactc
     1441 ttcaagtaag tcgctttact gggaagacag cgatcaggac ttcattgaag atacagacaa
     1501 gatcgttctc gaaaactata tccgtgtaaa cggtgtttca aaggacgagg cagaagctct
     1561 gttcggtaag gtgacttatc ttacaagaaa agacgctccg gtcggcgagc ttgcgtttat
     1621 cactcccgca atcagcgaaa aggcttgcaa cgaagctgtc gcaaagcttg gagataagct
     1681 gctcggtcgc ataagagtgc ttgattattg atttgaaatt tgtctttgaa aggaaaaaca
     1741 gtatgtccga aaatcagcca aagtataaaa gaatactttt aaagctcagc ggtgaagcgc
     1801 ttgcaggtga caagaagatg ggacttgata tgccgacagt taccgagata tgcaagagca
     1861 tcaagaagtg ctatgatgtt ggcacggaga taggtatagt tgtcggaggc ggcaactatt
     1921 ggaggggacg ttcaagcgag aatatggaca gagtaagagc tgaccatata ggtatgcttg
     1981 ctaccgctat gaatgcgctt gctgtagcag acgtgctcga aggtcttggt tgtgcggtgc
     2041 gcgtacagac agccatagat atgaaacaga tcgctgagcc gtatatcaga cagaaagcgg
     2101 taagacattt tgaaaagggc agaatagtaa tattcggttg cggtacggga agtccgttct
     2161 tctcgactga cagtgcagcc gcgctgagag cggccgagat aaatgccgat atacttctca
     2221 aggcaactat ggttgacggc atctacgata aggatcctca taagtatgac gatgcagtga
     2281 agtacgattc gctcacacac cagcagatac ttgtccagag ccttaaagtc atggacagcg
     2341 cagctgccgc tatgtgccgt gagaataaga cacctatcct tgtattcgat ctcagcagac
     2401 ccgataatat ctttgatgct gtaatgggca agaagatagg tactgtggta agcgataagt
     2461 aaataataaa acgataatat atacccgaaa ggaaatttac nnnnnnnnnn atttaccatg
     2521 aaagacgtaa tcaataccgc taaggacaaa atgggcaagt gcgtggcttc tgttgaaaca
     2581 gagtacgctt cgatccgcgc aggcagagca aatcctgcgg ttttagacaa agttaccgtt
     2641 gactattacg gtgctcccac acagataaac gctatggcgg ctgtagctgt ttcggaagca
     2701 agagtgctcg ttatcacgcc ctgggacgct tcaacgctga agtctatcga aaaggctatc
     2761 cttgcaagcg atatcggaat tactcccaca aatgacggta aggctatacg catcacattc
     2821 ccccagctta cagaggaaag aagaaaagag ctttgcaagc aggtaaagaa gatcgccgag
     2881 gaaggcaagg tagctgtacg caatgttcgc cgtgatgcta tggaaaagtt caaggctatg
     2941 aagaagaaca atgaaataac cgaggacgat ctcaagagct gcgaaaagga cgttcagaag
     3001 ctcacagata agtactgtga tgaaatggac gctgctgcaa ccgcaaaaga aaaagagata
     3061 atgacagttt aattatcggt aatcaaattg gctgatataa agaacgaagc tttaccttgc
     3121 gttccgctcc atgtaggatt tatcatggac ggaaacggaa ggtgggctaa aaaaagagga
     3181 cttccgagaa aggcgggtca ttcgcagggc gcaaaggtgt tcaggcgcac tgttgaggac
     3241 tgccgcgagg tcggaattaa atactgcact ttctacgcgt tttcaaccga aaactggaag
     3301 cgtccgaagg atgaagttga cgccattatg aaactgcttg aaaaatatct tgacgatata
     3361 cgcagtatgg cacagaagaa cacaaggata atctttatcg gtgataagtc tgcttttgcg
     3421 gaagatatac aggcaagact tgttgagata gaagaaatat caaagaataa cgacggtctg
     3481 tatgtgcttg ttgcgctgaa ttacggcggc aaggacgaga tattgactgc cgcaaagcgt
     3541 cttgcaaggg attataagga cggaaagata aaccttgacg actttaccga ggacgacttt
     3601 gacgattatc tctatacaaa gggcattcct cctgccgatc ttatcataag accgagcgga
     3661 gagcagagga tctccaactt tcttatatgg cagggagctt atgc
//
LOCUS       DAWAOG010000163         3703 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3168_length_3703_cov_1.997807, whole genome shotgun sequence.
ACCESSION   DAWAOG010000163 DAWAOG010000000
VERSION     DAWAOG010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3168_length_3703_cov_1.997807"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    330..419
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1459..1468
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1050 a          758 c          949 g          846 t
ORIGIN      
        1 tttacgaaat tcagagcggg acgatcactt acgacggtat cgatattcgc agaatcaaaa
       61 aggatgactt gaggaaatcg ctgtcgatgc tgattcagga tactcatttg tttaccggaa
      121 cgatcgcaga taatatccgt tacgcaagac ccgatgcgac aatggaagaa gtaatacagg
      181 cggcaaaaag cgcaaacgct cacgatttta taatgaagct ccccgaaggg tacgataccg
      241 ttaccggaag cggcggcgta agcctgtcgg gcggcgaaaa acagcgtata tccatcgccc
      301 gggcgctgat acagaagccg aatatacttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
      421 taagatacag agtgcgatag ataatctcaa gagcggcaga acgataatag cgatagccca
      481 ccgcctttca acgctccgtg acgcagacaa gctgtatgtg atcgaacacg gcgaggtgaa
      541 ggaaagcggt acgcacgatg agcttatacg cacaaagggc aagtactttg agctgtacaa
      601 gctgcagagt gagtcgctga ggtctgtcgg acttgactga taacgaaagg agagcttatg
      661 acgcagaata cgactgcaat ttatgatgta gatacactta acattctttc cccggacaaa
      721 atcggactta ccgaaaacga aagcggttat ctgtcgcttt cctatgacgg aaaggattat
      781 gaaaaggtcg accttacaag gcttgtgcct tttaacgatg aaaacaggta tataagcgta
      841 agctataaga atgaggagga tgagtggaag gagataggtg ttataaaaga tctgaatgaa
      901 ctgacagatg atgttaaaag tacggtcagt gcctatctta agctgaagta ctacattccc
      961 gagataaaaa agatagataa gataacggat aatcagatgg gctacctttt ccttgaagcc
     1021 gaaacaacgg cgggtgaaaa gaagatagcg gtgtacgact ggtggcacaa tttcagagtg
     1081 atacacgtta agatgcttgc tgtgacagat gctgacggaa accgctacag cgtacccgat
     1141 gttgacaggc ttgataaagc aagccttaaa aagctgcaat tgtttatctg attttagggc
     1201 ttttcagaaa ggagcgggtg taaatgaatt taaaactgtc agtcccgaaa ggagtggacg
     1261 ctgacagcgt gaacttttcg cttccgtttg acatagaccg gaaagcaaaa acggctgacg
     1321 gacatctgac agccacaaag gatatgctga cggtatatta tgacaataaa gcggtacggg
     1381 agttttctgt atcgaatata agcaagataa cggtagaaca gcttatcggc tgttcaatgc
     1441 tgtgcgtaag ggatatgcnn nnnnnnnncg gcaaggatat ctgcgtctgt cagttttcgc
     1501 agaagtacta tatgcgctat gcggagcttg cgaagatata cgagcattac atagccacgg
     1561 gtgaatttac cgagcataca gacgcggatg agcctagctg tcctaagtgc ggagcggcgc
     1621 tcaggggcag cagtaaatgt atgttctgcg atgcgaaaaa gggcgtgttc ttaaagcttg
     1681 taaaaaggct tgcaccgtac aaggtgcagt tctgcctgtc gcttctggct acattcgtac
     1741 tgtatgcgtt tgacgttatc aatccgctgt ttcagcgtat actggtagat gaccttatcg
     1801 tgccgaataa cagcgactgg ggacttttct ggaagataac gctgtgcatt ctgacgctca
     1861 atctgtcctc tatgggcttc aggctgctgc aggaggtatg taattataag atatcaacgg
     1921 catacggcag ggatctgcgc cgcgatatat tcaacaagac tcagcggctg tcaatgagca
     1981 atgtcgcaaa acgcactccc ggcgagctta tcaaccgcgt atcgggtgat gcggcgactt
     2041 tgcaggattt tatgacaagg cagggcaagg atatgatatt ccagacgaca gcccttgtcg
     2101 ctctgcttat aatcatgtac attactaact ggaagctggc tcttgtcatt actatcccct
     2161 taccgcttgc ggctttcctt tcggtaaagt cgttcaatgc gataagcgta agatatggca
     2221 gagtgtggcg cttccagtgc cgtgcgagcg agcttctgca cgatgttctg cacggaataa
     2281 gggtagtaaa gaactacggc aacgaggaca gggagataga agcgtacaga aaagcctcat
     2341 caaagtgggc tgactctgtg atgagagctg atatcctctg gtatatggta cagccgccga
     2401 tccgctatat attcagtata ggcgagttct gcgcactgtt cttcggcggc agtatgatac
     2461 tgggcagaga gatgcagctc ggtgagctga tacagttcac gacctatgtc gctatgctct
     2521 acggtcctat ccagtggctt acaagcctgc cgagagtgct tgcacaggca cgggtaagtg
     2581 caggaaaggt gtttgagata cttgaagagg ataatgatat aagcgacacc gaaaacgcag
     2641 tcagccttga cataaagggt gatatcgagt tcaagaacgt atatttcggc tacaaggcgt
     2701 ataatcctgt gctcaaagat atttcgttca gcataaagca gggcgagatg ataggaatag
     2761 tcggacactc gggtgtcgga aagtcaacgc tgataaacct tatcatgcgg ctttatgacc
     2821 cgacagcggg cgaggtgctg atagacggca aggacgtgcg ggatatatcg cagaactcac
     2881 tgcgttcgca ggtcggcgtg gtattgcagg aaacgtatct gttcaacggt acggtgcttg
     2941 acaatatccg ttatgcaaag cccgatgcga cattcgagca gatagtgaat gccgcaaaga
     3001 cggcaaactg ccacgacttc atcacccgta tgccggacgg atataatacc gttgtcggcg
     3061 aaaggggtta caatctctcg ggcggtgaac gtcagcgtat agcaattgca agagcaattc
     3121 tccacgaccc gaagatactg atacttgacg aagctaccgc atcgcttgac actcagaccg
     3181 aaaagcagat acaggacgct ctcgacaggc tgataaaggg cagaaccaca attgcgatag
     3241 ctcacagact gtctacactt gcaaatgccg acagacttat agtgctcaaa aagggcaagg
     3301 ttgccgagat cggcactcac accgagcttt tacgcaataa gggcgtatat tatgagcttg
     3361 taatggcaca gaagcagacc gcaaggctca ggaagagtga agcggctgtg ccggttatgg
     3421 ggtaaaccga taatcggata aagtatagtt accgatgtgc acctgcatat gtgggtgcgc
     3481 gttgtttgta tacggcaaca cacctcgctg ttattatgct gtttaggctg tagaaaactc
     3541 attaaaaata cagcacaata tattattgat taagcggtga tttagatata atgccggtac
     3601 tgctgaaaac agtttctgtg ctacacagtg aataataatt ttgtgagtat atcggtgcaa
     3661 ctgcactatc accgaaattt ccaaaaaacg cactatccca aga
//
LOCUS       DAWAOG010000164         3685 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3188_length_3685_cov_2.370523, whole genome shotgun sequence.
ACCESSION   DAWAOG010000164 DAWAOG010000000
VERSION     DAWAOG010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3188_length_3685_cov_2.370523"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          824 a          915 c          837 g         1109 t
ORIGIN      
        1 ccgccgataa agagggcgat ctcgggtccc ttgcccgcaa gcgttgcttc aagtactgaa
       61 ccctgtacga ggtttatcga agcctgtgta ccgttgtagt cggggtttga gttgaacttg
      121 ttatcaacaa ggttttttac gactgttgcc tggtcacgcg caagtgatac ccatacatca
      181 agtgatgttg cgcttgcgtc cgaaaggttc gtgtaatctt caaagaacga accgataaac
      241 gcattgaagc cgaatgccaa ttcactgaag aaatttccgt agggtgaagc aaagtcctcg
      301 tgacaggttg ctatctctat gtagtcaagc tcaagaggct gaccgcgata gtcacgcatc
      361 catgcggata ccgatgagat gctgtccttg atgctgcttg ccattacagg gatatcttca
      421 ggcttgttga tacatctttc gaggtagata cacatcgttt caagtgcggc cgcctcagaa
      481 cccttctttg taaggctttc gatgcttgcc ttctctgcac gaagcgagtc aactattctc
      541 tggaacgcat cctgaatacc gggtatcttc ttttctacaa agtaatcctt gtattcatca
      601 ggatcgggac ctgtgatcat aagtattctt ctgtagtact ggttgagctc aagcacgagg
      661 tcgtcgagtc tttgcataac ctcgcctatc gaaccgggta ttgcttcaag tgtcaatgtg
      721 tgttcctcac ctgccgtcag gtaaacatac atatcgtttc cttcggcatc ctgaggagtg
      781 aggttgtacc aatccggaga gtagatgaac tgtacatcgt caaattcctt acatggtacc
      841 ttaccgtcaa tgtaaatcct tctgtttgag aagaatccac gcatctggtt ctgtcttgac
      901 ttgaagctga aacggtagta accgtcattc tcaaccttga agttgtaagt gaccgcctgt
      961 gttgatttgt tccatgttgc ctgaccgata gtgttataac gctgtttggt cgggtggttg
     1021 ggacttgtca tataggtcgt tctgtcgtaa gtagcatata atgttgatgc tgtcttgtag
     1081 agcgcctctt ccgcctgtaa gaagatagtg tttgtgccaa gctcctcatt ctttgaggaa
     1141 agagcgggag tgttctgcag ctgttcgtct gtaggcttta tagagtcgta gcttacaagc
     1201 tcatcgtaat tcttgaacgt aaacgaatgg aatacgccat aggttcttat gccctcaagc
     1261 gtaagcgtgt gagtaccctt ttcaaggtag aagtaataag gctcgttgta aagaccttcc
     1321 ttatccttga taggatattt tatccaacaa tcataggtca cctgaccggg tctgaggtcg
     1381 ttgtccttgt tgtcacgggt gatctctccc tcgtccttcc agtaacggtc gagtgtgaga
     1441 tcctgtgtag cagtaaaagg atattctccg tcgatcctga gtccgatatc cagtaccgta
     1501 ttttttgttt ctttgccctc gataggatag taaatagtct cgaggttgta aatgcccgtt
     1561 tcctcaacgt taaactcata tgtaagtgtg agcttatcat ttttccactt tattgtagaa
     1621 taatgatccg tcttgaggtc ggtgttctct gcgaggaatg acttgagtcc gttgtagccg
     1681 tcgccggatt tttccttgat ctcttcgacc gagggaatga tctttgcggc ttcttcagct
     1741 gtcattgaag acgaaccgct gtttacaaag gatgtgccgt ctatcgtacc ttccttgtag
     1801 tcggtagcat agacagtgat ctccttcttg ggctttgcgg catccttatg actgttgtaa
     1861 taagtgctgt acttgccctc cataccggtg atcgtaattc cgctcacaga gctatcggtc
     1921 gtcgtgcttt cctcggattg tccggcagaa gtatcggttg tttcttctgc gctgactgcg
     1981 gttgatgtaa gtgacaatga agatataagt gtcgcacatg acaatatacc cgctaatgct
     2041 cttttcatcc tgcttgattt tgttgccaca gtaataatcc acctttctga tagttgtcgt
     2101 ttatgttcaa tcgaaaatat gctaatttcg acgagttaag gaatttatcc gtgcgagtta
     2161 taatccgagt tgtagacaga tatttttccg tcttcaagct caacgcgcac tttatctccg
     2221 cctcttatgc cgagggcttc gagatattct tttggtatct ggagtctgcc cgctctgtca
     2281 agaacgacca gctcctcatg atccgaaccg ctctgttgca taagcttttc atgctctgca
     2341 cgctgctgtt cgctcatctt ttcgagttct ccgaaggaga ggatctcggt tttctttctc
     2401 agcatctcgc tcgatgtgcg tccgtcacgg atagcgacca cacggtcgat cgatttcgac
     2461 agattagtat catgcgttac tattataatg gtaacgccca tctgacgatt gagctgtttg
     2521 aaaacatcaa gcaccatagc cgccgtcttg gtatcgaccg cacccgtagg ttcgtccgca
     2581 agaagcaggc tgggattgtt tgaaagcgct atcgcgatag cgacacgttg ctgttcaccg
     2641 cccgaaagct gaccgagaag cgagtttttc cttgcggaaa gcccgaccat atcgagcagc
     2701 tcttcggctc tctgcctgcg gttcttatga cccgaaagca gaagcggcat ttcaacgttc
     2761 tgtatcgccg taagatacgg aatgaggttt ctggcgttgt tctgccatac gaaaccgacc
     2821 gtttctcttt tatattttat aagatccttt tcgtcaaatt tcagaagatc cttaccgtca
     2881 acgtaaagac ttcccgccga cggcttgtca agcgctccga gcatattcag cagtgtcgat
     2941 ttaccgctgc cgctcgcgcc gactattccc atcagctcgc cgcgctctac agtgagatcc
     3001 agtccctgaa gcgccatgac ctctacgtcc gtggtcttat atatcttgac aaggttctca
     3061 cattgcacca tcacgttttc agggggtgca agcatcattc tctgaggcat cactccacct
     3121 gctttctgac atctgtaatg cgtccgtcgt caagtgtgta aacaacatca cactgttcca
     3181 tcattgcggt atcgtgcgtt gtcatcacga tcgtaagacc gtctgtatgc acaagctctt
     3241 tgaaaagatt catcaccgca agacctgtcg gcatatcaag cgccgcagtc ggctcatcgg
     3301 caaatatcag ctttggcttg tgtgagatgg ctcttgcaat agccactctc tgctgctcac
     3361 cgccggaaag ctctccgggt ctgtgatgca ttctcttttc cagacctact cgtacaagac
     3421 attccttcgc acgttttaca cgctcggcat aaggcattcc gacaagcctc atagaaaact
     3481 caacgttttc gtatgccgtc attgtcggta tcagcgccac cgactggaac acaaacgaca
     3541 tatcgtttct tctcagctta tccatttcct tgtcggtaag ccctgttatc tttttaccgt
     3601 caaagtaaac atcgccgccg gtcggtctgt caagcgcacc gagtatattt ataagcgtag
     3661 tctttcccga acccattctc cccac
//
LOCUS       DAWAOG010000165         3684 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3189_length_3684_cov_2.573161, whole genome shotgun sequence.
ACCESSION   DAWAOG010000165 DAWAOG010000000
VERSION     DAWAOG010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3189_length_3684_cov_2.573161"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1051..1060
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          824 a          982 c          820 g         1048 t
ORIGIN      
        1 tgtcctcact ccgacaagcg cttcaaggtc gctgtcgacc tgctgtaaat gattttttgt
       61 ctttgtcagc acactttcaa agttctcaaa ttctttcttg acaccgctga gtatcttcca
      121 tacctcactg cttcgtttct gcacggcaag ggtgcggaag cccatataca atgagttcag
      181 caatgcggac attgtggacg ggcctgcaat gctcaccccg tagttacgct gcagctcctc
      241 caccagtccg agctttacga cctcggcata caatccctca aacggcagga acattaccgc
      301 aaagttggtc gtgtacggcg gacagatgta cttatcgtga atatccttcg cgcatttctt
      361 tattgccaca acaagcgcct tcgatgcttc ttcgatcatt accttatcgc cgctgtcgta
      421 ggcactgcaa agagcggtgt aggtatcgcc ggggaatttt gagtcgatcg gcagatacac
      481 tgtatcgtca ttcttgcccg ggagctttac cgcaaactca acatggttac ggctgtcggg
      541 gacgaccgcg acctcggtat catactgctc gggagccaga atgtcggaaa ggatcgcgcc
      601 gagctgcatt tcgcctagta tacctctgtt ctttacgttg gacaggactt ttttcagatc
      661 gcccacccct gcggcaagac tctgcatttc gcccagtccg ctgtatacct tgctcagctg
      721 ttcgctgacc gttgcaaacg aacggcttat cttgtcgtca agcgtagtct gcagcttttc
      781 tccgaccatt ccgcgcattt cttcaagctg atttttcgta tttgcattta tgtttccgag
      841 ctgaaccgcc atttcgttac gcatattctg catatcggcg gcatttctct gctcaatgct
      901 ctgaagccgt tgctcgaact gtgacagcag ccttgcagtt ccctccgcat tctgcttctg
      961 cgtgtcggtg agcgtacctc cgaggtttgt cacaaggctt acaagctcgg ttctgagcgc
     1021 cgtttcgccg cttgcacggc ttgcctgcac nnnnnnnnnn tgcctgcaca gtatcaaatt
     1081 ttgccgaaag ttgcgcagta cgctccgtaa gcacatcaag ctgttttgcg gtctgctcgt
     1141 ccgttatctt gctcacgccg atattttcgg caagcctttt ctgcgtaaga agcattatta
     1201 ccgatattat caccgatatc agactgacgg caagggttat tataagcagt atttccatat
     1261 tccttactcc ttttaaaatt tatactacta ttatagataa ttttttctgc agggtcaacg
     1321 ctttcacaga tgagtgtact gtttatggga caaaaaatac ggtcacccga aggtgaccgt
     1381 acaatctgta taaacccgtc tgatatagct tttgacaaat gtgctgtacc gcaccgatat
     1441 cagcagtcat atacgacagt ataataaagc atttgacggc aaatatctaa gaaacaaatc
     1501 agatatgcag tttatcgacc tgctccttaa gcagccttgc ctgcgcagaa agctcctcac
     1561 aagaagcggc agactcttcg gcggtcgcag agttggtcgc aataacgcgg gatatctgat
     1621 ccactcctac tgtcacctga cggactgcat ctgcctgctc caccgccgcg gctgaaatat
     1681 ccgcaacaag ttttgcgctt tcctgagaaa gtgctgttac ctgacgaagt gtatcggcag
     1741 tctgatttgc tatatcagag ccgttatgta ccgcctcgat agttgatgct ataagatcgc
     1801 gggtgttgtt tgcaaattct gccgacttag acgcaagatt tcgtacctcg tccgcaacta
     1861 ccgcaaagcc cttgcccgct tctcctgctc tggcggcctc aatagccgca ttgagtgcga
     1921 gtatgttggt ctggaatgca atatcctcga tggacttgat gacctgcgag atcgcttcgg
     1981 actgttgctg tatcttatcc atcgcttcaa gaagatgctc catctcttcg ctttggcgta
     2041 tcatctgctg tgcacagttg tctgaaagcc cgctcgcctt gcctgagttc tctgcagtct
     2101 tattgatgtt gtcggatata tccgatatag tggaagaaag ctggtcgact gctgtcgcct
     2161 gacgtgtagt tccgtctgcg agaacctgcg aaccgtcagc catctgttgc gagcctgccg
     2221 taacattgtt tgcggaagaa ttcatactga gaataacgcc gctcaacgtt tcctttatgc
     2281 tgcggaatga gcggtttatc tcctcaaagc tgccgatgta tcccatcgaa ggctgtgccg
     2341 aaaagtcgcc tgttcccatc tgtgacagca ctcgtgaaat atcatcaaca tagctgttca
     2401 gcgtgtgctt tgcttcagtc agtttttcgg ctatgttgcc catttcgtca tggctgaatt
     2461 ttaatttgga atcgggcgca ttaagttcac cggatctgag ctgaccgcag atatttacga
     2521 cctccgttac cggcttgcgt acaagcttcc tcattactct gaacagcacc gccgctatag
     2581 caccgcatac aacaacaagt actcccagtg agagcaggat agacttcatc agctccccat
     2641 ctgccatagt ggttgtatag ccggcaaaat aagcgcctac gacctcgccg ctgtaatcca
     2701 ccataggtgt gtagttgaca taatagttcg tatcgccgat gaccgttttt ccgatatatg
     2761 tattattgtt ctgtacctgt tgccagatac cgccgtccat ctgagttccg atgttgcgtt
     2821 cgcccgaaga gttcatcagc gttgtattgt aacgtgtatc accgcagaac agcgtaagct
     2881 ccgcacctgt ctttgccttg acagaatcga cataatccga gccgttcagg tcaatacaga
     2941 taaccagtgc accattctcc atcttaaggc cgtatacagt atacagctta tcgccgtcag
     3001 ccataatgcc ctttatttca ccgtccgtaa tgcgttccac agcgctttca ccaagcggaa
     3061 agccctcact gctccacgcc gcagctccgc cttttatata agccgcatag ctgttgtcgt
     3121 tcggtttgtt ggcgttccaa agactgtcat aatctatatt attcgcctga ccgtctgagc
     3181 ttgcaacgat cagctctgcc gcatcgccca tttcgtctat gtcgccctcg acctcagcgg
     3241 caagtacgtt tatacctgtt gccgtttcgt cctcctgtat ggttttgaca aatgacataa
     3301 acacgattat agtgaccagc gccatggata ttgcggccat aacggcggca aaacacgaga
     3361 taatcgttat ttttgttccg atcctcattg atcttttcac tgtaaaaatg catcctttca
     3421 tatatataat aatatcaagt ctgcggttct ccgcaagccg tgcggggcac ggcaagatcc
     3481 gctatcggcg gattttgtgt ctgaggttat tataacataa aaacaagttg gcgtaagccg
     3541 acacggcggt acgccgtctt ttgcctttcg gcaaaagctt ttatgttaaa atgttctcag
     3601 accggcggta cgccgccgac cgtgcttttg cacggcaaga tccgctatcg gcggattttg
     3661 tgtctgaggt tattataaca taaa
//
LOCUS       DAWAOG010000166         3676 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3201_length_3676_cov_2.118752, whole genome shotgun sequence.
ACCESSION   DAWAOG010000166 DAWAOG010000000
VERSION     DAWAOG010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3201_length_3676_cov_2.118752"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          966 a          822 c          769 g         1119 t
ORIGIN      
        1 acataggcgc gcctatgcct gcaggaagtc caaccgccgc atactctata atgccgccta
       61 cagaatctag atttgcagat gcgttagata taatatccct catctgctgt tcaacgttgg
      121 tattgataag cggaagatag cggcttctga cttgctttaa ttgctcagca ttcacgttta
      181 cagggtcaaa gcgttcatcg cctgcgtcca ttattgacag gatatgcgct cctgaggtta
      241 ttcccttgct ttcaagtatc tgagatgcga tagcacccgc aaaaaccagc ggcgcggtaa
      301 ttcttcccga aaaatgtcca ccgccgcgca ggtcgttact gccgttataa cgcacatatc
      361 ctgtataatc ggcgtgaccg ggtcgtgcaa ggtggctcac gctcttataa tcagccgaat
      421 gctgatctgt attctgtatc acagcgcaaa ggggagtgcc tgtcagcaca ccgtctacca
      481 ttccggatac tacatttgga aaatcctttt cactgcgcat tgtgcttgtg ccgtcttttt
      541 taggcgcacg tcttgccata aagtcaagca ccgcgtcaaa atcaatcttt attccgcccg
      601 gaagcccgtc tatcacaaca cctattgcct taccgtgaga ttctccgaat attgaaacag
      661 aaagctctcc gaacttaaac gctgatgaca tctgctatac ctccgagtgc tctgaaatcg
      721 tcaaaaaatc cgggatagga cttgtttgta cattcagcgc cttttattat tacatcgcct
      781 gttgagcgtg tagccgcaac agcgcctgcc attgctattc tgtgatcgtt gtaattgcgt
      841 accgtgccgc ccttgaacgc ttcaacaggg aatatttcaa tgctgtcgct ataggcaacc
      901 gcattaccgc cgaggtcgtt tataagctcg gtagtagccg ccagacggtc gcattccttg
      961 atgcgtaatc tctcgcagtt gattatcctg cacggctttt tccagaagct catcattaca
     1021 gcgatcgcag ggacaaggtc gggaatatcc gatgcgtcta ttatcgacag atcaccgccg
     1081 tttttgaact tgtcggcgat atcgattata gccttatcgc cctgaacact gttcatatta
     1141 aggtttgcaa gctctatatc tgagcctgcg cagtttgcgg caataaagaa tgcgctctgc
     1201 gacatatccg cttcaacagt gcagttatgc ggctgatatt tctgaccgcc ttttatgaag
     1261 tagccgtttt ccgtttcggc aacttcaacg ccgaactgtt tcatgcagtc tatcgtgata
     1321 ttcacatatg gtcttgattc aagccttgtg gtaagaatta tctcgctgtc ttctttcagt
     1381 acagaaagcg caaataaaag ccctgttata aactgcgatg atacactgcc gtcaatataa
     1441 tattttccgc cgtgaagctt accttttacc ttataagaca tggtgagcgt ttcaaatgtc
     1501 gctccgttgt cgatcatcgg cttaataata ggtgttatcg gtctttccgg taattttcct
     1561 ctgcctgtaa actcagcctc gcaaccaaac gcagagaata tcgggatcat aaatctcagt
     1621 gttgaaccgc tttcaaagca gtctacagct gctgtttttt tcggatttac tattcctttt
     1681 acatcatagc ttccgttttt gccttttatt tccgctcgga gtgcggtcag cgcatttata
     1741 gtagcgtgtg agtccataca ttcaagcagg ttatctatat gagtcatacc gtcgcataaa
     1801 gccgcgcata taagcattct gtgcgaaatg ctttttgacg gcggtacgat aagcttacct
     1861 ccgagctttg tcggtgttat ttttatgtcc attatgttcc gcctttgatc cgaatttatc
     1921 agaataatca tactataaaa cagaaaaaaa gtcaagacgg ataaaccgtt ccgacatgca
     1981 taaaaagccg attgcagagg ataagcaacc gactttatgt attatttttt acttgcgttt
     2041 tcaagaactg tcttaagcgt tcccgtcagt aaggtagaca tcttcgtgat aagctcatcg
     2101 gggtcgctct tcattcctcc ctcaagccaa tcaagcacta tccccactat accgtatttg
     2161 tagaatgatg ctataaaatt tttatcctca tccgatacat taagttcaag ttgctttgat
     2221 gcatttccga tgaacttatt cgcgaaatcc agtgaaacac tgtaaagata acgctcaatc
     2281 gtttcacggt gcagtgaatt gtaaacgtgg tagacaagct tcttgttttc cttgacaaaa
     2341 caaagccctg tctttagccc ttcctcccat gactgaccga tcaccatctc atctaccgac
     2401 ttttgtgctt ctgtaaggaa gatctcttca aggagctgat atatatcgct gtaatgatag
     2461 taaaacgtgt tacggtttac tccacagtcg tctgttatat tcttgactgt tattttatct
     2521 aaaggctgag tactcagcag ttttttgaaa gattcctgta tagctctttt ggtaagtgcc
     2581 gacatataac ctccgtttta gctttagttc agctgttttc ttttttccgg tacatattat
     2641 acaacatttc tattaaaaat tcaagtttta ggcaaaaaag cttgtttgcc caaaaaacag
     2701 tcaattttta tcgattgcct gaaatttgaa cattgacacg cggttgactg ttgtagaaat
     2761 agtgaaaacg ggataaaata tagctgtaaa gaaaaagaaa ggactgaaaa ccatgagatt
     2821 tataaaacgt gcaaaagtag cttatatttc tgcttgtgcc gcaatagcgg tcatcggact
     2881 tatactgata attttcccgg agatctcaat gcttgccgta tgctatatgc tcggtatttc
     2941 cgctatagta ttcggcatcg ttaaaatcac gggttatttt tcgtcagacc cttacgggtt
     3001 ggcgttccag tttgactttg cattcggtgt catagcaata ctgctgggtt tgatcgtaat
     3061 acttcatccg ggcaatgtaa tggctctggt acctgttata atgggtattt actttatgat
     3121 agacggcata atgaaaatcc agactgctgt cgatgcaaag cgttttggcg taaacggttg
     3181 gtggctgata cttgtcagtg cgatagtcac gggtgctgtc ggaatactgt tactcataaa
     3241 tccttttgag agtgcggtag cccttacggt tctgctcggt atcacattgc ttgcagacgg
     3301 tttatccaac atatggatcg ccgcttacac agttaaggcc attaaaaaag aaatacacgc
     3361 cgatgacaaa atttatgtag aaacaagcga tagggaggac aaagatgatt aagctaggca
     3421 cagagtaatc attcttattg ttgcggtagt attgctgata ccctcggtac tcggtatgat
     3481 cgcaacaaga ataaattacg atatgctaaa ttacctgccc gatactatgg aaaccataca
     3541 gggtcaggat attctgctga atgacttcgg caagggcgca ttcagtatga taattgtgga
     3601 ggatatggaa ccgaaagatg tttccgctct taaagaaaaa attgaagcgg tagatcacgt
     3661 tgattcggtg atctgg
//
LOCUS       DAWAOG010000167         3673 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3207_length_3673_cov_1.720011, whole genome shotgun sequence.
ACCESSION   DAWAOG010000167 DAWAOG010000000
VERSION     DAWAOG010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3207_length_3673_cov_1.720011"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1499..1508
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2842..2851
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3176..3246
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          927 a          907 c          703 g         1045 t
ORIGIN      
        1 cccattaaat accttccttt ccgatttgcg gttacatact tatactgtca accgcatcat
       61 agcccataag cagtatcgac ccgccgactg ttataaagtc ggaatagctg tctgtaagca
      121 gtgtcttttt ttccgattca agagataaat tatattgagt tatatcggat tcggtcataa
      181 taaaaacagt atcaccgctg acttttatct gttttacatc ggctgaaacc gcaatggagg
      241 ctgtcacttc acacttgtca ttgagaataa ccgttttttt gccatcatca gtgaagtcat
      301 caaacacaaa cgctttaaga gatgatgatg cgtcaaagtt ctcgaccgtt cccggatagc
      361 tgtagtttcc gataagttca ccgctgtcgg cgctgtatgt agccgccata ttgtcaccgg
      421 ttacagtaag cacgtttccg ctaagttcca gcgaaagtga tacacagtca cccatcgact
      481 gcgaccacaa ctcagtgccg tcaccgctga tatcatactt tgagagcttc gtgacaatat
      541 taccgttatc ggatgttacc ctcgtcacat agagatacct gttgtcatct gagaatacag
      601 cctgcattac attgtcatca ataaagcgct gtgtgtaaag ccactttccg cttccgtcat
      661 agacgtatat tacatttgag tatctgccgc ctgttgttac gaccgcagta tgctcagagt
      721 tgctcagaaa tacggatact ataacctcgt cctcgacagt ttccttatag atctgacttg
      781 tcttattaaa cagcgaaaaa tcatgtccgc ccttatcgta gataaccacg cgctttgagt
      841 tcgacactgc catagggtga acatagccat gctgtaaagc aaaattctgt ccgccgttct
      901 cgttatagga atagaggtag gtatcactca gcagtgcaaa gctgtcattc atcgcacaga
      961 agctgtagtc agaccttccg gtaagactta tcggatagcc tgcggtatcg gtagtcgtcg
     1021 tctgaatttt agaagcgata ccgcgcaacg gctcaaatat cgtgtcacgg tatacccata
     1081 tcggtacagc agcagcaaca actacaagca atatgataag gtttcttgtc agctttgctt
     1141 tcttcttttt tttgcgatac agcgtaacat cgtttatgtc gttgttttgc tgttcaagag
     1201 gatttttatc gggtactttc atatttaccc ctctttcatt tatacatctt ttagtagaac
     1261 cccttgttga gatacagtcc gcaagcgggt acagtcttgc cggcaaactc cctgtcgggc
     1321 tttgtgagcg cgtttattat atcctgctcg gtacgcttgc cctcgctgat ataaataaga
     1381 gttccaacta taattcttac catattgtga agaaagccgt ttccacagac tttaatttcg
     1441 acaaaatcgc ccgtctgctc aacatctatc gaatagacgg ttctgacggt ggtcagcann
     1501 nnnnnnnngc agatgctctt tagcttccgc cttgcagaat gaagcaaagt cgtgctcacc
     1561 gaccataagc cgcgccgcgc ttcgcatttt ttcaatatcg ggatcatacg gataatgaag
     1621 cgccatatcc gccgtaaata cgtccctgac aacacctgta tctatcctgt agatatactc
     1681 cttgccctta ctgtcaaacc ttgcgtgaaa gctgtcaggc acttcttcac acgaaagaaa
     1741 agcaatatcc cgggggagca ttgcattccc gccctttata aagccatcac agggaatagg
     1801 acaagcagtc ttcacattga acacaaacgc tctagcgtgc acacccgtgt ctgtccgtga
     1861 acagccgttt atcgtgatat cctctccgag cagttttgac aacacctctt ctaccgtctg
     1921 ctgtatgctg tttgcgttgt tctgccgctg atatccgtga tacgccgtgc cgttatatgc
     1981 catagttact ttaagattgc gcataactca ccttacattc cggggacggg aacataaatg
     2041 ttcatgagaa cggtcaatat gatcattgct gccattatac ccgtagcaac aatatccctt
     2101 gcaccgaact tgagctgctt cattctggtc ctgccctcgc cgcctctgta gcaacggcac
     2161 tccatagcca gtgcaagctc gtttgcgcgc ttgaatgctg atacaaacaa tggtatgagc
     2221 accggtataa gcgccttggc tttctttaca agtccgcctg tatcaaggct cgcacccctc
     2281 gccttctgtg ccgacattat cttgtcggtt tcctcaataa gagtagggat aaatcttaaa
     2341 gctatcgtca tcatcatagc aagctcatgc accggaaaat gcagtttctt gagcggcgac
     2401 aacagtcttt ctattgcatc tgtcagtgct atgggtgatg tcgtataggt aagaagcgac
     2461 attccgacta taaggaatac aattcttatc gtcataaaca cggaggtatt gataccctcc
     2521 atatatattg ttataaatcc gagctgtacg agcggatcac catcaccgtg tataaaaaac
     2581 agattaagta tacaggtgaa cagtatgatc ggcataatag gcttcaagct cttgaatatc
     2641 agcttgaagc ttatccccga catcatataa attatcagca tgaacagtcc cgccgttata
     2701 agcgaaaaat aattccctgc cataaacagc ataacaacat aggctatatc aagcactatc
     2761 ttgaagcggc tgtcaagtct gtggattatt gaattgcccg ggaaaaactg tcctattgta
     2821 atatccttca gcatatcgtt cnnnnnnnnn ncattattct ctcttttgca cgttcaacag
     2881 tatatatatc ttttcctaag tctacacctt tatctctcag cttcatcatg atctttgtga
     2941 tctgaggaac atcaagacct atctttataa tctcgtcctg ccttgcaaat acgctgtccg
     3001 tatcctcatg gcagaagagt ctgcccttgt tcattacaag aaccttatca gcatacttta
     3061 ctatatcctc catagagtgg gatacaagca gtatcgtctt gccgtatttc ttatgataat
     3121 cgcttatctt accgagcacc ttttctctgc ctatcggatc aagtcctgcg gcgggnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnncaag cgctacgcgg cgcttctgtc cgcccgaaag gtcaaacggc gaacgttcaa
     3301 gaagctcctc atggataccc atattttctg cagcctctcg caccctgcgg tcaacctcgg
     3361 cttcgtcaca gcccatattc ttaggtccgt atgctatatc cttgtatact atttcttcaa
     3421 acagctgata ctcggaatac tggaacacaa gaccgacctt aaatcttatt gagcgtatgt
     3481 caacaccctt gtcccatatg ttctgtccat ctatgagcac ctcgccggat gtcggcttta
     3541 caagaccgtt aaggtgctga ataagcgttg attttccgct tccggtgtga cctattattc
     3601 cgcaaaattc gcccttttct atagtaaggt ttacattatc aacagcggtc gactcaaacg
     3661 gagtgcccac gct
//
LOCUS       DAWAOG010000168         3663 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3219_length_3663_cov_2.254435, whole genome shotgun sequence.
ACCESSION   DAWAOG010000168 DAWAOG010000000
VERSION     DAWAOG010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3219_length_3663_cov_2.254435"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2026..2035
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          801 a          891 c          837 g         1124 t
ORIGIN      
        1 gcctgtaaag gtacgtccgt tttcctcaaa ggtcatactg catataatag gcagatcgca
       61 gttttccttt gcggcaagta tagcggcttt aagctcgtat aagtcggtca tcgtttcaaa
      121 cagaacaacg tccgcacccg ccttatagcc tgcaacgatt tcttctttga atatgtcgta
      181 agcctcttca aatttaaggc ttccggtagg ctcaaggagc tgtcctatag ggcctatgtc
      241 aagagcgcag aaaacgtcct taccgctttc ctttatcgct tctttacagt ttattattcc
      301 tgcggtgata atttcatcta cgcttttacc gctgtggctt agcttgtagg aatttgcgcc
      361 gaatgtattt gcggaaatta tatccgcgcc tgcgtctata taactgcggt gtatttcttt
      421 gagcagatcc tttgcggtaa ggtttaaaag ttcgggtgtt tcgcccgttt taagcccctt
      481 agcctgcagc tgagtaccca tagcaccgtc gaaaatgagc agtcctcttt gttcaagtaa
      541 tgttttaatt tccacagtga cctccgcttt ttctgaattt gcatctgtct ttcatattac
      601 agctttcaca gcctcttgta cttctgtcga cctcgccctc ggacagtccg ataaatgccg
      661 ttaccgattt tgtaggcacc atcatattgc tgttcattac agtaagacct atccttttct
      721 gagcctgtaa aagcgtcagt aaatcaaact gtatgtcaag cggcaagtca ccgtatccgg
      781 gagaatatct ccaggtacgg ctgtttgatc ttactttttc aaatatctct gtttctgccc
      841 tgttgcagac ctgctctatc gccgcacctg caaggctgtc aacgataacg gcgcttgcca
      901 tatccgatac ctgcaaacgc cttaaaagca catctactcc ctgtccgagc gtggcgcaca
      961 taagcactat cctgtcgcag ttctttaaat gagcggcggc tgagctgcct ttaagtatta
     1021 tattgctgtc cgaaattctg acaccgtcat tcgtaaagct tatcgggtaa acggcataga
     1081 gatatttcgg acttgcggct tttaaaagct ctttttcgca taaatcgagc attgtaagca
     1141 tctgctcgtt atccgtgccg tccttacagc cgagataacg cagagcttcg tttctgtcta
     1201 tcggctgaag tatcataaca gatccttgat gccattgtag atgcgtgttg caacagcagg
     1261 gttattcata gcgtaaaggt gtatgccttg cacatcgttt gagataaggt ctactatctg
     1321 gtcgatggca taggctattc ctgcactcct cagcgcttcg gggtcattct cgtacttctg
     1381 catcatcttt acgaattttg atggcagact tgcgccgcac attgttacca tacgctcgat
     1441 ctgctttttg tttgtaacgg gcataattcc tgcggcgata ggtacgtcta tgcctgcggc
     1501 tcttgcacgc ttttcaaaat cataatagca ggaattgtcg aagaaaagct gtgaaacaag
     1561 gtgagatgcg cctgcgtcaa cctttatctt caggtttttt atatcttcct cggcgctctc
     1621 tgcgtcaacg tgggtttcgg ggtagcacgc acctgcaata tcgaaatttc cctttgacgc
     1681 tatatatgcc gtaaggtcgc ttgcgtgggg aaagtcaaac ttaggctctt catcgggctt
     1741 gttgtcgcct cttaaggcaa gaatgttctc tattccgttt gcggcaaaat cgtcaagcat
     1801 aaaatcaaca tcgtcacggg tgctgtttat gcaggtaagg tgcgccattg cttcaatgcc
     1861 gtatttttcc tttatatgcg acgctatctc gcaggtggag cggtttgcac ggtttccgcc
     1921 tgcgccgtat gtcacgctga tatatgcggg atttattgcc gcaagtccct cgatggcatc
     1981 atacaccgtt tcaatgggaa aattcttgtt gggagggaaa acctcnnnnn nnnnngtcct
     2041 gccgggtgtg tataagtctt taattttcat ctttttgttc ctttatgtca ctcgttgtct
     2101 tctgcgaatg tttatcttac cgtactgccg ttgggtgtat ctttatcaag gaatacgacc
     2161 tgtatctttt ctcctgcatc gcaggcaagg agcataccgt tgctttcctc accgcagagc
     2221 tttgcggggg caagatttgc aacgaatatg attttctttc cgataagctc ctcgggagtg
     2281 tagtattcgg caatgcccga tactatctgt ctgccgcctc ttgcatcgtc cacctgaatt
     2341 ttaagcagct tctttgactt ctttatcttt tcgcaggcag tgatctcgcc tgttctgagc
     2401 ttgacctttg caaagtcgtc tattgtgata attcctgcct tgtcaaggtc ctcgtcctcc
     2461 ttgattgcgg cctttggctt catcaggatt tcaagctctg aaatttcgtt cttgatatca
     2521 attctcggga agagaactct gcccttcttt accgcaacgt ctgcacgcag aacgccgaat
     2581 gtaccgagct tgtcgaaagc ggtatcttct gcacctgcgc ctatctgttc ccatacctca
     2641 ggcattgttg cgggcataaa gggactgagc agtgcagagc atattcttat agtttcaagc
     2701 aggttataga gtactgtcgc aagacgggcg ttgttgcttt cgtcctttgc aagtacccac
     2761 ggtgctgttt cgtctatata cttgttggca cgggagatta ccttgaatat ttcctcaagc
     2821 gcctttgagt gctgagcctg ctcgataagg ggagtaaccg tatcgcacag cgactgagcc
     2881 ataccgataa gctgattgtc aagctcgtcg gatactctgt ctgtgggcag tgttccgccg
     2941 aagtatttgt ctgccattgc gaccgttctt gaaacgaggt taccgaggtc gtttgcaagg
     3001 tctgcgttta ttcttgtgag cataagctcg tttgtgaagt tgcagtcact gccgtacggc
     3061 atatcgcgca gtatctgata acgtacagcg tcaacgccgt aacgttcgca aagcacaaac
     3121 gggtcaacaa cattgccctt tgacttgctc atcttgtcgc cgttgaagtt tatccagccg
     3181 tgaccgtaaa ggtgcttggg caggggcaga tcgagcatca taaggattat aggccatacg
     3241 atggagtgga aacgtacaat ttcctttgcc gtcatgtgca tatctgcggg ccagtacttt
     3301 tcgaagtcgt tgtacttctc atttccgtag ccgagcgcac tgatatagtt tgaaagcgcg
     3361 tctacccata cataaacaac gtgcttgctg tcaaaatcaa cgggtatacc ccaggtgaag
     3421 ctggttcttg atacgcacag atcctcaagg cccggcttta tgaagttgtt taccatttcg
     3481 ttcacacggc tcttaggctg taaatagtcg gtttcggtaa ggaacttttc gatcttgtcg
     3541 gcaaagtcgg aaaggcggag aaagtatgct tcctcgtttg cttcttttac ctcacgtccg
     3601 cagtcgggac atttgccgtc aacaagctgg ctctccgtcc agaagctcta acagggtgta
     3661 cag
//
LOCUS       DAWAOG010000169         3642 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3250_length_3642_cov_2.128798, whole genome shotgun sequence.
ACCESSION   DAWAOG010000169 DAWAOG010000000
VERSION     DAWAOG010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3250_length_3642_cov_2.128798"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3075..3084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          962 a          884 c          729 g         1057 t
ORIGIN      
        1 gcctgcgacc ccgctctttt gagcggggtc ttattttgta gaaaaagtca gaaatgccgc
       61 ccaaataggc ggcacagatt ctgttattta cttgttattc ggcagctttg cgtaatatct
      121 cgccactgtc tttgcagaat cctcgctgtg ctgtacaaac catatcttat caacgtcaaa
      181 caggatacat ccgcagtgcc ctttgaaggt cggaaagcat attaccttaa gatacgtatc
      241 tatctcgggg ctgtagtcca taagctcaag cgtttcgccg taaagcgtgg tacgttcata
      301 tcctctcagc cacttagcgt ccatattgct gaacagcgta ccgaatgatt gcccgaccag
      361 cttttcgagc gggagctttt ccagccttgc aagagcttcg tttgcatagc ggaatatcca
      421 atctaccgct ttacactcct cgttgaatac catctcaata tcggtaaacg caaacggcat
      481 agcatcaaag gagcggtaat aatcgtgata ctgctcttcg gtatccggca caccgttatg
      541 atcgaagcct tttattatcc tctttctgct tgtctgcagg ctttcgatta tggtattctt
      601 cctgcggcgt gcatattcga gcttttcgcc gtttacaagg tctatcatat cggatatttc
      661 gtgaatagcc atagccgata cgatgcatcc tctgtgtgcc ttgataaagc cgtcacccag
      721 catctcttca agagcggcaa aggtcattct tgttttatat atctttccgt cagacaggtg
      781 tatctcggct gttttgcctt ccattactat gtacagtata gagctgacgg aaatcactac
      841 tttctgccgc ttgcttatta ttgtgatata ctcagattta ttaaaattag tcaaggtgac
      901 tccaccattc tttcgcatat tactgccata taattcacat tatacgacat tccgtgctta
      961 attgcgatat caatgaacct tttttcactt aatacgcaca ttgcccggct gtaaaagcat
     1021 atctcagctt attgtttctt tataagcata tccgcaaagt attcgcacag ctgagggttt
     1081 cttatcagca caggaccgat aagatgagtg ccgtagaaat tgccgtaatg cacaccctct
     1141 tcgctgtcgt ccttgccgtt tccgcttccc tgctttacat caaacagcgg tgaggtagct
     1201 ccggttgtaa ggctcgcctt attcacaaag cctattatat ctccgccgca cagcgatgtg
     1261 ccgcaaaggc tgtctgtgac aattctatct ttcccttcaa ccgtttcgta aggaaagaac
     1321 gaaagacctt cgtgctttac gccgtcacag tcggtaacgc tctgtccgaa catctcaaac
     1381 gaattgcctg ttgcaagaat gaccgtaccg ttatcaagtg ccgctttgat gttatcctta
     1441 tagccttgca gatattccag tgctaccttc tgatttcttt cggtcgcaga accgataaac
     1501 acaagatccg cacccgaaac atctatctca tcatctatcg accgatattc gacttcaacg
     1561 ttctgccctt tattttcaag tgctcttttc aagccgcata cattaccgta atcgccgtaa
     1621 agattgcaca gatccgcaaa taaatgaatt atcctcatac gtcctcctct gcctgagtaa
     1681 gctctacctt tgacaagaac ttctccttat ccgaaaagca ggtgacggta tacagcttgc
     1741 ctagcggttt agccgcaatt tcatcaagtg cctcaggaat atcctcgttc acagtcacct
     1801 tagctatatc aatatccgta tacgaaagcc ttgtttcaag atcctttgcg tgtctgccga
     1861 gcagataaag atgctgtatg cagtcggctt taagcacacc gtagtcaata tcccacagcc
     1921 agcttgtttc tcctgtcgaa taacgacggc ttaccgaatc tattattatg atcaccgagc
     1981 agggttgatt ttttcttatg atataatcat acacgcggtc gcttgccaca gaattttcgt
     2041 gctttgcgat aaggaatgta ccgttgttca taccaagttt aaactgcaaa atacgtccgc
     2101 tcttaaggaa ataatgtgac agttcatctg ctatgacctc gccgtcaatc ccgacaatag
     2161 agcaagccgc aaagcaggca agaatgttat aaacgttgta aacgctcttg aacagtagct
     2221 ttatcttata tttgccgttt atcgtaacaa taccgctgtc ataatcgaca ttcgttacgg
     2281 tgtactgcgt atcgcgcttt ctgtgtccgc agttgtcgca gtgatagctt ccgatatgtg
     2341 catagtgata gtagtcatag ctcattctgt gcttacattc ggggcagaac gcaccgtcat
     2401 tatatacgcc tgtagcgtgg tctgtgctga aatcctgctt atccataccg aaccatatca
     2461 cattatctct gtccttgccg tatctcgaaa caagcggatc atcggcattg agaataagct
     2521 gtgattcggg tctgatactg tccttaaccg attcatagac ccattcggga tgaccgtttc
     2581 tcgtaagctg gtcacggtaa agatttgtaa ttatatagtg cgtaggagca aagaatttga
     2641 acgaatactt tgcgtatctc tcgtccgact caagcagaag aacatcgccc tttaccttac
     2701 cgccgaatgt gcagttggat aatattatcg ctgtaacacc cgctatctga tttgagccct
     2761 ctttattcca tataaccttt tttcccgatt tacgcattac ctgtgcgatc atctcaacgg
     2821 tcgatgtctt gccgttgctt cccgtaaccg caatgacctg ctgaggcagt ttcacccttg
     2881 acagtacatc ggggcagatt ttaagcgcta tatcgcccgg cagactgctg cctctgccga
     2941 taagtttaag taaacatttc gacactttgc aagcaagtat cgctaagaac attctcatac
     3001 tttctatctc cttaaagaaa ccgattctaa gattcataat ttaaattata tcacaaaagt
     3061 gagctctttt aaagnnnnnn nnnnatttta gtctgcctat caaaaatgtt aatatcgtca
     3121 tataatttct gagaataaaa caagacgaaa agacaacaga agtgctaaaa taaaggcaac
     3181 ggtgcaaagc accaaaaacg ttttgtattt tacagaaagg aaatatttta tcatgagtga
     3241 aaacagactt ataggcgatt atcccgtaat aggcatacgt cccacgatcg acggcagaag
     3301 aggtgttctc aaggtaagag aatcccttga agagcagaca atgaacatgg caaagtcagc
     3361 cgcaaaactg ttcgaggaaa atatcagata ttcaaacggc gaacccgtaa aggtcgttat
     3421 tgccgacact actataggca gagttgccga ggcggcagcc tgtgcggata agttcaagaa
     3481 gtgcggtgtt gacattacac tcaccgttac tccctgctgg tgctacggcg cagaaacaat
     3541 ggatatggat cctatgacga taaagggcgt atggggcttc aacggcacag agcgtcccgg
     3601 cgctgtttat cttgcgtctg tacttgcgac acacgcacag aa
//
LOCUS       DAWAOG010000170         3622 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3279_length_3622_cov_2.274741, whole genome shotgun sequence.
ACCESSION   DAWAOG010000170 DAWAOG010000000
VERSION     DAWAOG010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3279_length_3622_cov_2.274741"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          920 a          861 c          868 g          973 t
ORIGIN      
        1 cgtcgacctt gccgttgtcg gcaatgatcg cgtcacacgc cgagtgaccc gtacagcggt
       61 tgttgccctc caccaccgaa tagaacgcct gatgtgaatt tcctcttgcg accgagcgcg
      121 aaacaagatc cgcaccgctg tcctcgccgt tcagctcaac gtggaacttg gttaccgctt
      181 cttcgcagcc gtcagtcatt atacgttcgc ggacaactat ctttgcgccc ttgctgagca
      241 ccgcagaggt gcttcttgtc gagctgtcaa caccgcggag ctgtatcgta tccatttcga
      301 gataggaatc ctcaccgagt acacagtcgg tcaccgggtc gatctttttc agacccttgc
      361 ccttacccgt gccgatatgc ttttccttgt aaagcacctt tgagcccttt tcaaggaaaa
      421 agcggtgtat gccgttgtga cgcgcctgtt cctcgccgtc attgtgtacg ccgcagcctg
      481 ctacgataac aacatctgcg ccctcgccga tatagaagtc gttgtacaca aggtcgtcaa
      541 catcactgtg ggtaacgcag gcggggatat aaaccgtttc agctaccgta aacggcttta
      601 cgcggatatt tataccgggc ttgtcgtcct ttggctctat agttatattc tccgatgatt
      661 ttctgccggc gcacttgctg tcctcacgga tattgtatgc accgttgaag cctgtaactt
      721 catcataatc ggaaatgatc ttcagcattt cggctgttgt gccgttgaaa tttacgtcca
      781 ttatattgct cactgacaac cgcctccgtt tctctgggga catctgcaag tatattcgcc
      841 gccgagaagc ttcggaagta tttcttctct cggacctgcc gtgcggactg tgccgtccgc
      901 tactacaact atttcatcgg ctatcgaaag tatacgctcc tgatgcgata ttataacaag
      961 tgagcctgtc gtgccgtttc ttatctcttc aaacgtttcg accagtcttg caaagctcca
     1021 taggtcaatg cccgcctcgg gctcgtcgaa taccgaaagc tttgcgtttc gcgcgagaac
     1081 cgtcgctatc tctatacgct ttgcttcgcc gcccgataaa gcactgctca tctctctgtc
     1141 gatgtattcc tccgcacaaa gtccgacctt gcccagaagc gcgcacagct cattatgacc
     1201 gagagcgttt cctgcggcaa gctccagaag atctcttacg gtaagtccct tgaagcgcac
     1261 aggctgttga aacgcataag ctacgccgag cttcgccctg cctgtgatat ccatatcggt
     1321 gatatccgta ccgtcaagta taattctgcc cgatgtcggc ttttcgacac ccgcgatcag
     1381 ctttgcgagc gaggtttttc cgccgccgtt aggtcctgtt atcaccgtaa gcctgctgtt
     1441 gagcttacag ctgatacctt ttataatagg ctctccgctt tggagctgcc actttacgtc
     1501 ctgaaattcc agcaaaatta ttgccccttt catcttttat catattatcg taccatatta
     1561 gcgttattat gtcaagagct gttcgtttct attgaatttt tccctgcttt atgataaaat
     1621 attatgatga taaatcggga aagggtgaca gtatgaaagt atctattatt gacaacagtc
     1681 tttgcgatgc atcatttgtt gatgatgtaa agccggaggc tgttgttgca tataaagacg
     1741 cgcttgccga atttggagta gcgtatacgg aaatgacgac cgacactttt cttatgcttc
     1801 ctcctgcggg agatatgtca aaggtgatac tgcgtatgac ctctgaaagg gatctgcttt
     1861 atataaattc gttcaacttt gcatatgtaa cagtacctgc caatctcaca gagctgatac
     1921 ccgggataga aagacccgtg atatccgaat tatcgctcca cgggggcgac tccctgcgtg
     1981 ctatggagat gtttgaaaag aatttcgagc ttgataatgt gtcgatgata cgctttgtgg
     2041 acgacttcag ggagtcgccg caggaaatgg caaagcttat acgggaatat cgtgataagt
     2101 attttcgtcc tgtcgatatc tgtccgacga ataaatatac aaatgccgta aatgaggcaa
     2161 tagctgctgt aattgctaaa agcgacagca taaccatgcg ctttggcgat tatgaacggt
     2221 tcgccgagct tcaggactac accatatcgc ttgcaactct gttcggtgtc gctccgtcac
     2281 cgcagatgat cctggcgctg tataagtgcg gttgcctgta cgctatgata tacggcagaa
     2341 gagcaaggag cactcttgac gacctgcgtt tcagccagat atcaccgcat tatgtaagaa
     2401 atgcggacag atatgtgccg atgtcaccca acgagcgcac aacgcataca tcatttgtac
     2461 gtccgccgga caattccgca cagaacagcg atcttttgta taagaagctc cgcagtatga
     2521 tggtagacgc ggtaacagca caggagcttg aagaagtgat agacgagttc tgcacacagc
     2581 tttacaataa atcggagact aagaaaaact gaagctgcac gaaacatcag ttatcgccaa
     2641 ccgactatat gtataaaaaa tgcaagtgac aacaagtata tatagtgtaa tttgacgaaa
     2701 aatattgaga gataaaaaaa tccttttatt ttcactgcgg atatgatata ataattctat
     2761 actgtgtcga tgtgctcagt acgagcaagc gatatggcat ctgattttga tatcaagccg
     2821 tattacggca ttactataat ttgtttacat atttttatag aattgaggaa gtgtgacttt
     2881 tggaaaagtt tattataaaa ggaggcactc cgctcagagg cgaagtgcag ataagcggcg
     2941 ctaaaaatgc ggcggttgcg ataattcccg cggcgatact tgcggcagat aagtgtgttc
     3001 tggaaaacgt accttcgata agcgatgtag aggtagcgct ggagatcctt tcaagtatgg
     3061 gtgcggtcat caaaagactc agcaagtcaa agatcgaggt cgatacgtca agaatatgtg
     3121 acggcacagt gccgtacgag cttgcacgtt cgacaagagg aagcagttac ttcctgggag
     3181 cactgctcgg caggttcaaa aaggcgagcg ttccgcttcc cggagggtgc gatctcggaa
     3241 gcagacctat agatcagcat atcaagtgct ttgaagcgct tggtgcaaag gctgatatca
     3301 agcacggaat agtagacctg tctgcggaca agctgatagg tactcagata tattttgaca
     3361 aggtaacggt cggcggcaca ataaatgcac ttcttgcctg tgccaaggcg gaagggctga
     3421 ctattatcga gaatgcggca aaagagccgc atatagttga tcttgctaac ttccttaatt
     3481 caatgggcgc agatataatg ggcgcaggta ctgatgttat caaggtaaag ggtgtttcaa
     3541 agcttcatgg cacggaatat tccgtaattc cggatcagat agaagcggga acgtttatgg
     3601 tagcggccgc ggcgatctgc gg
//
LOCUS       DAWAOG010000171         3613 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3286_length_3613_cov_2.040191, whole genome shotgun sequence.
ACCESSION   DAWAOG010000171 DAWAOG010000000
VERSION     DAWAOG010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3286_length_3613_cov_2.040191"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1185..1194
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1032 a          847 c          836 g          888 t
ORIGIN      
        1 gtcaaggaat atgtagttac acgctgtacc gaggtgcttg aaccgctcgg agttatcttt
       61 gtcggaagtc acccgatggc aggacgtgaa ttttcgggct ttgattattc gaccgatgat
      121 ctgttcaagg gcgcaagcta tataatcacc ccctccgaaa atacccccaa aatggcaata
      181 gatctgctgt caacgcttgc gtgctgtatg ggcttcggca aggtcgtgac cgctaccccg
      241 aagcagcacg atataaacat agccttcacc tcacagcttg cacacgtcgt ttcaaacgct
      301 tatgtcaaaa gcccgtcgct tttcaatgca gacggatttt cggcaggcag ctttatggat
      361 cttacccgtg ttgcaaagct caacgaatat atgtggagca gtctgttcct atgcaataag
      421 gaagctcttt tgttcgagct gaacaccatc atcgacaatc tgtgtgaata cagggatgct
      481 atcggcaacg atgatgttgc aaaacttcgg gatatactcc gtgacggcag agagcgcaag
      541 gaaaagagca acgaacttta tgcagagcac gcaaggagaa aggtttaaaa tgccctcaaa
      601 caataaaaac agaagctacg ccatagcgct tagcggaata ctgagcgcta tatgcatagt
      661 acttatgttc tgtacaggct ttatccctat cggcgcttat atcctgcctg cggtagcagg
      721 tcttgtcgta tggataatct accgtgaaat aagcaggaaa tgggcgcttc tttgctatgc
      781 ggcagtagct ttactcgcac tgcttctcac cccggatata gagtcaaagc ttctgttctc
      841 cggatttttc ggttactatc ccgttatccg tgacctgctt acaaaagtta aacctgctgt
      901 actgtcattc ttactgcgtt ttctttgctt caacatacct gtcgttgcaa tatacgccct
      961 gcttctgaac gtgcttggta tgggacagct catcgaggac tttgccgggt tcggacagtt
     1021 tgcggtactt gctttctggc ttatcggaaa ctttacgttc atctgctacg atttttgttt
     1081 gagccagctg tcgtatgttt atgaaaaatg gctgaggaga aaggtacaca ggcttatacg
     1141 gtaggaatac gatacactta tggcggctga agcggctggg acggnnnnnn nnnntcgaac
     1201 ttctcaccaa aatgcttgaa acaagatatt tacccgatta actcacacaa aacgataatg
     1261 tgttgttgac attcgtgtca ggttaaggta taataatagt attataaaac cgcatggcgt
     1321 ttgcctatgc ggttttgagc gttaaaaacg cttatagaaa ggtatggaac aaatgcaaaa
     1381 caaaatactg tttacagctc cgggcgaatt tgacaagtgg gagcagcaat gtcagcctat
     1441 cggaaacggc tatatgggag caagtttctt cggtggcatt tcaaaggaaa aaatagttct
     1501 taatgagaaa acactgtggg caggcggtcc ttccgagagc cgccccgatt acaacagtgg
     1561 tattatagac ggtagctatg aatatgttaa acaggtacag cagcttctct atgacggcaa
     1621 gtatgacgag gcggtcgcac ttctccccca tctgacaggc gcaactgacg gcttcggagc
     1681 atatcagtta ctatgtgacc ttatgcttac tttctcaaat atagacgaaa cccttgcaac
     1741 agattacacc agaacgctcg accttgataa ttctttattc acaacacaat ttacatatca
     1801 gggtgcagtt cataagagag aagctttcgc gaactatccc tcaaacgtga tctgtctgaa
     1861 attaagctct gacaagcccc gcaggatatg tgtaaagctt tctcttgaca atctccagtg
     1921 cggcagcgtt accgcaaacg gtgatacact tacatatgag ggtgctctat gggacaacgg
     1981 tctgcgttac tgcaccgtat tcaaggtggt aaacaaaggc ggtgagctta ttgatgcaaa
     2041 ggactgcata atggtagaac acgccgatga ggtatatatt taccttacag catcgaccga
     2101 ttattcaaat aagtacccga ccttcagaac aggtgtcaac ccgaggatcg cggtaaaaca
     2161 gcgtatcgaa aatgctgtaa acaaaggcta tgacgagctt tacaaggaac acctttcaga
     2221 ctacaaggca ctctttgaca gagttacgct gaaaataaat gaagatacag atgatgttat
     2281 cccctgcgac aaactgataa gcgactataa ggaaagcggc acaagaagca tagcaaaccg
     2341 ccttgaaacg ctttacttcc agttcggcag atatatgctg ataagctcat caagagcagg
     2401 ctctctcccc gctaaccttc agggcgtgtg gaacgagagc aattgccctc cgtggtgttg
     2461 cgactatcat ataaacgtaa acctccagat gaactactgg ggcgcatata acacgaacct
     2521 cagcgaaaca gtacctcccc tcgttgattt ccttgacagt atgcgaccgt ccggcagaaa
     2581 gtccgcagaa gcctactacg gcataaaatc cgatgaacat catcccgaaa acggctggtg
     2641 cgcgcataca cagagtacgc ctttcggttg gacaactccc ggttgggact tctactgggg
     2701 ttggtcgact gcggctgttg catggcttat gcagaacatc tacgaatatt ttgagttcac
     2761 aggtgacaaa acgtactttg aagaacacat atacccgata atgcgcgaaa gtgtacgctt
     2821 ctatacacag tggctgatat atgatgacaa gcagaagcgc cttgtgtcat ctcctactta
     2881 ttctcccgaa cacggacctg ttacaatagg caacacatat gagcagagcc ttatcgaaca
     2941 gctctatagc gacttcatca ccgcgagcga ggcgctcggc gaggacgaag agctgcgcaa
     3001 taccgtaaaa gaacagatag tacagctaaa gccttacagc gtaagcaaaa aaacgggact
     3061 gctcaaagag tggtttgaag aagatgatga taatttcgac cacagcaaaa ctcagaaaaa
     3121 tcatcgtcat atctctcatc ttctgggtct ttacccgggc aagggaatca actcaaacac
     3181 tcccgagctt atggaagccg caataaacac tctcaacgac cgcggtgacg agtcgaccgg
     3241 ctgggcaaga gcgtacaagc tcaacctttg ggcaagagta aaggacggca accgcgctta
     3301 ctcgatttta cagggactgc tgagaggctg tacatacgat aatcttttcg atttccaccc
     3361 cccgttccag cttgacggaa acttcggcgg cagtgcaggc attgccgaaa tgctgataca
     3421 gagccacgag ggctatatag aactgcttcc tgcttccccc gatgcatgga gaaacggtag
     3481 cttcaaagga ctttgcgcac gacacggctt tgtagtagac gcaaaatggg aaaatttcaa
     3541 tcctacagct gtaaccataa agtcgactat cggcggcatt tgtcacataa aatcgaactg
     3601 tgaagctata ctc
//
LOCUS       DAWAOG010000172         3598 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3301_length_3598_cov_2.305391, whole genome shotgun sequence.
ACCESSION   DAWAOG010000172 DAWAOG010000000
VERSION     DAWAOG010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3301_length_3598_cov_2.305391"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          835 a          832 c          783 g         1148 t
ORIGIN      
        1 gcttaaaagc ctgtgtttat gttctgcggc atatcgtgct gttatacaat gactttcaca
       61 gctttcatat gctctttgta ggcggcatca ataagtgcta tcgcctgttt tctgttatct
      121 gcgttttcaa aataaagctt gatggttctg tcgagaaagt tgacataatc agcaatagcc
      181 ttggcggtaa gattagggtc ggatactttt cgtgtatcct taagaatagg cttattaagc
      241 attctcgtca tcttgtcatt atctatctgc atgattatat caatatcggt aaactgtgcc
      301 agatttcggc gctcgatgct cgcaaagcag gtgagaaagt cgttcatcag cgtcacaagt
      361 gccgcattct gcgttctgaa gctgactttc aaccgtccga ggtacttgcc ctcgcgcata
      421 agctctaccg aataacgtat tgtcggcttg tatttataag gcagcgggct tttgataagc
      481 atacctccat cgcccgccgc agtctgcaag cggaatatct cctcttcaaa aaaactgctg
      541 atattctcca ttatgttacg cattcgtatt tcgggcgttt gcattgagag cttttcggca
      601 agtatcctgt cgataagtgc cgagcggctg gtattcattc tgtatgccag cgagtctatc
      661 gctcgtacta tctcatctga cagcaccagg ctgtatacac ttttactcat tttttgacga
      721 tcattccttt cgattaagct atctatatta acagcctttt tctgactgtg aacatattgt
      781 agcacacttt tagtaacttg tcaagaggtt tatataaatt tttttacgag ttatgaaaat
      841 attttttaaa ttattgagga ttgcgccgaa aaattgcctt gtaaagccga tttgcctata
      901 catacaaaaa tccccctccg attatctcgg agggggattt gatgcaaata ttatcagaat
      961 gccttcttgt agatttcgag tatctcatcg ggatttgttt ctctgggatt gccgcctgtg
     1021 catacatcgt tgaatgctgc tacggacaga tccttgaggt cttcttcctt gacattgatc
     1081 tcgtggagct tctgaggaat gtttatagac agtgacagct tgcgtacagc ttcaacagca
     1141 gcctttacgc cttcttcatc tgtcatattt tctgtaccct gtacgcccat agcctttgct
     1201 atagtcttgt acttgggctt tgcggggctt tctgcgttat attccattac ataggggagc
     1261 agaagtgcgt ttgcaacgcc gtggggtgta tcatagaatg caccgagagg gtgagccatt
     1321 gagtgaacga taccaagacc tacgttcgag aagcccatac ctgctatgta ctgagcaagt
     1381 gccattgttt cccttgcttt ggggtcgccc tgaacactgt cgtacagtga gcctgctata
     1441 agctcgatag cacggagctc aaccatatcg gacagctccc acgcaccttt tgtgatatat
     1501 ccttcgatag catgtgtcag tgcgtccata cctgttgatg cgcacagacc cttaggcata
     1561 cccatcataa gatcgctgtc tacgatagct acaacgggga tatccttggg atcaacgcaa
     1621 accatcttct tcttgtttgc ttcgtccgtg ataacgtagt tgatagtaac ctctgccgct
     1681 gtacccgatg ttgtgggaag cgcgatcagc ggaatactct tgttctttgt atctgcaacg
     1741 ccttcaagag atacaacatc cgcaaagtcg gggttagcta taatgatgcc tacagccttt
     1801 gctgtatcca taactgagcc gccgcctaca gctacgagtg agtcagcgcc catctccttg
     1861 aattttgcaa cacctgtctg aacatttgct actgtggggt ttgccttaac gtcactgtat
     1921 atttcatagg caattcctgc cttgtcaagc tctgccgtaa cctttgtagc cactccgcat
     1981 tcaagcagta ccttatcagt aacgaacatt acctttttaa gtcctctgcc tgtaagctca
     2041 ggaacgaggt tttctcttgc gcctgcgccg aaatatgaag tttcgttaag tataaaacga
     2101 ttagccattg gtctttacca tcctgttctt tgaatttaaa gcacggaata tatatttccg
     2161 tttgctgtat tcattatact acctttggat agaaaatgca acacttttca caaaagtttt
     2221 acatttatcg tgggtaatat atgtaaaagt tacattaaag gtatttcttg taacccggat
     2281 gcttgcctgt aacgccgtat tgctctctca tgccgataag tctgtcgatg ttatcggggg
     2341 tgatatcctt tacgccgccg agcagtcttg ctacaagaga aatatgagcc tgaagctcca
     2401 ttgtttccat tctgtagtat gctgtgataa ggtctgcgcc tactgtcagt gcgccatggt
     2461 tttcaaggag catacagtcg tgttccttta agtaaggcgc aattgcttca ggtacttcgt
     2521 aggtcgaggg cgtaccgtag ggagtgagcg gtattgagcc tactccgatt acagtttcta
     2581 tcatgcaata atcatccatt ggaagatgag cgcaggcaaa gccggtcgaa acgggaggat
     2641 gagcgtgtac tactgcgccg acatcgggac gctccttgta gcatctcagg tgcatcttta
     2701 cctccgatga gggacggaag ccctcagcgg cttcaagtat gttgaagtca tcatctataa
     2761 gacctatctt ttcgggagtg ataaatgcct tgctgacacc tgtctgcgta gcaagatact
     2821 tgccatcggc tacctttacc gttacattgc cgtcatttgc ggcaacccag cctttctgcc
     2881 agagcttgtg gcagatatcg accatctgtt cttttatttc ttcgtacata tttatatcct
     2941 ttcggttgtt tattttatct gtgtttatac aacgcccttc tggagcatta tgtttgcgta
     3001 tacggctttt tcgcctgttg cgattatcgc ataggcggtt ttagcctgtt cgtaaaattc
     3061 aaaacgttct atctgtccga tagcgttgtt tcctctttcg tcgtgctttg cgactattgc
     3121 tttgtattcg tcccatatag gagtttttgc ggtgtcgccc ggcattacct gcataagatt
     3181 tacaggcttt tcaacgtaag tatcaagagg gaacagcgtc agtatcgcgt caagcagctc
     3241 gggtacgccg tgaccgtccg cacggataac cttgcagttt ttacccatac tctcggcagg
     3301 gaagttgccg tctgctatta ttattctgtc gctgtgaccc atttcacaaa gggtcttgag
     3361 cagttcgggc gataatatcg caggaatatt cttaagcatt atttcacctt ctcaagatag
     3421 attttatagt atttgttcca ttccgaagcg ttttccggag tgaatgtata cagcttttcg
     3481 gaagcggcta taatctttct tgccgtttct atatctgcta tctccccgtc agctataagc
     3541 tgaattgcta tattgccgta agaggtagcc tcgacaggtc cgcttgaaac ctctatac
//
LOCUS       DAWAOG010000173         3597 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3305_length_3597_cov_2.645680, whole genome shotgun sequence.
ACCESSION   DAWAOG010000173 DAWAOG010000000
VERSION     DAWAOG010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3305_length_3597_cov_2.645680"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    322..331
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    943..952
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2260..2292
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1011 a          753 c          871 g          909 t
ORIGIN      
        1 ggataggatc aacgagcaca agcagaagca tacacgggat ataacctata aacggtccga
       61 aatacgggat aatgcaggtg atacccatta ttacgccgca gagcaccgcg taaggcatat
      121 tgaatatgct cataagcagg acatagacca tgccgataat gaagctgtct aaaatctttc
      181 cggtaatgaa ctgcccgaaa gacttgttta tttcacgcgt gctgttgatg atcctggtag
      241 cgactttggg tttgaacaga cagtaaagca cctttttgaa ctgagcagca aaaagctcct
      301 tgctgttgag gaagtagata gnnnnnnnnn ncacaaagcc gagcagcagg ttcatcagaa
      361 cgctgattat attcataacg tttgacgaga tcaaattcca gatattgttc atctgaggaa
      421 gaagctcggt agacagccag ctgttgagca tatcggaaat gccgcctgtg aactgaagga
      481 catatgcctc gacttcgggg tagtttctta gggtctgcga tacccagtcg cttacctgcg
      541 tgacataggt cggcatattg tttacgagca ttgtgatagt atcgataagc tgagggagca
      601 gcacccagat tatacctgtc agtacgagca gggcaagtat catggtgatc gctacgctca
      661 ttacccttgc gactttgcgg acggggaacg tctttggcgg cggctcttcg cccggatgct
      721 cacgctcaaa cttctcgcgc tttctgtcct ggacagtata aaacagtctg cgaagacacg
      781 gcttttcgat aaaagtcgct atcgggcaga gaatataagc aatgacaaag ccgtaaagta
      841 cgggtgtcag gatcgataag aatgagaata tgccgccaag tatttctttg atttgcgaaa
      901 gaccgagaaa gaatactatt gcggcggcga taacgaggaa tgnnnnnnnn nnctccccag
      961 tagagatatt tcttatccca tttgaaattc attacatcac acctttattt tgatcgtatc
     1021 cgaccttttt ggaagttact gcttttatta tagaattatc ggcggaagtt gtcaagggta
     1081 tacgatgaaa agtgcacaaa tgtgtgcgga tattgaagaa taaaaacgac actgcttacg
     1141 gttaggtaaa ttcatcaatt ttcataggtc ctgcggacct atttttgcgc tgtaaaggac
     1201 ttgacaagag gcgcttataa aggtataatt aaatcaatag taagcgtctg ctgtagttgt
     1261 gtttgcagta ggcttaattt ttaaccgcat acacggaata ctctgaggca agcaacgttt
     1321 aatcaagatt aaagtacgtt tgcctcaaag ttattccggg gttgcgttgc acaaaggtgc
     1381 aggaaaggaa aggtaaaact atggctttta tttactctga gccctcacac acattcggcg
     1441 agtatcttct cgtaccgggt tattcatctt caaagtgtat acctgcaaac gtatcactgc
     1501 gtacacccgt tgtcaagttc aagaagggcg aggaaagttc tatctatatg aatattcctc
     1561 tggtatcggc tatcatgcag tccgtatcgg atgacaagat ggcagtcgct cttgcaaagg
     1621 agggtggcat atctttcatt tacggttcgc agtctgttga ggatgaggcg gctatggtag
     1681 caagagtaaa gagctacaag gcaggctatg taaagagcga ctccaacctc gctcccgata
     1741 tgacgcttgc tgatgtgctt gaactcaagg ctaagacagg tcactcaaca atgcccgtca
     1801 caagcgacgg cacagagcac ggaaagctgt taggtattgt aacaagccgc gactacagag
     1861 tatcgagaat ggctacagat acaaaggtat ctacatttat gacacctttt gaaaagctca
     1921 taaccgctcc cgcaagcaca acacttaagg aagcaaacga tataatctgg gagcacaagc
     1981 tcaactcact ccccatagtt gacgataacg gcgtacttat gtacttcgta ttcagaaagg
     2041 actatgaaca gcacaaggaa aacaagaacg agcttctcga cagcaagaag cgctacatag
     2101 taggcgcagg tatcaacaca agagattacg cggagcgtgt tcccgctctt gtaaacgcag
     2161 gcgctgacgt tctgtgtatc gacagctcag agggttactc cgagtggcag aagctgacca
     2221 tcgactggat ccgtgcaaat tacggtgaca gtgttaaggn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nncttcctcg cagactgcgg cgctgacttt ataaaggtag gtatcggcgg
     2341 cggttctatc tgcattaccc gtgagcagaa gggtataggc agaggacagg ctacagctct
     2401 tatcgaggta tgcgaagaac gtgacagata ctttgaggaa acaggtgtat atatccccgt
     2461 atgctcagat ggcggtatcg ttcatgatta ccatatgacg ctggcacttg caatgggtgc
     2521 tgactttatg atgctcggca gatacttctc acgttttgac gaatctccca caaacaagct
     2581 gttcgtcaac ggcagttatg ttaaggaata ctggggcgaa ggctctaacc gtgcaagaaa
     2641 ctggcagaga tacgatatgg gcggcgacaa gaagctctcg tttgaagagg gtgttgacag
     2701 ctatgttcct tatgcaggca gcttaaagga caatgttaca cgctcactgt caaaggttcg
     2761 ttctacaatg tgcaactgcg gctgtcttaa cattcccgac ttccagaaga acgcaaagct
     2821 cactcttgtt tcttctacaa gtattgtcga gggtggctca cacgacgtta tcctcaagga
     2881 aaaccgcggc gctcgcagcg aataattgcg gtcgtttggg ttgccgcgtc cttgcggcaa
     2941 cttagtttcg accgctgaca agcatccgtg cttggcggcg tggggcgcgg caaattcgcc
     3001 gttctgactg ttgccgatag gcgacagtta cattaccggc ggacttaata tgctactgca
     3061 tattcaatta agacataatt aaaggatact gcaaacgctt taagcttgcg gtatcctttt
     3121 cgcttatgtg aaataaagtt tcttattgtg catttacgat atcacaattt agctcccttg
     3181 tgtaaaggtg agcttctgtt gctgagttaa actcagccaa ctcttgaggg attgttagcg
     3241 gaggtgttgg caaagaagga aaggttcggt gcaagcgatc cttcagtctt gcgacagata
     3301 aggctaagcg gatattaacg ttattgaaat cgcaaagtat atcaatattt aatatggaag
     3361 agccttgtcg caatccagct ccctttggca agggagccgg gaattagtgc ttggcggagc
     3421 atgttgtttt ttatcaaatt gttagagcgg ataaaaaaac aacgcggcag tataaaaaat
     3481 atatagccaa caccgctgaa agcatatcca taccgataag gtgttaataa tttcgtgagt
     3541 aaatcggtgc aactgcactg tcaccgaaat ttccaaaaga cgcactatcc aaagata
//
LOCUS       DAWAOG010000174         3591 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3312_length_3591_cov_2.768665, whole genome shotgun sequence.
ACCESSION   DAWAOG010000174 DAWAOG010000000
VERSION     DAWAOG010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3312_length_3591_cov_2.768665"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1400..1409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          845 a          890 c          690 g         1156 t
ORIGIN      
        1 tacgttaagc ttgctttcag cccttttata tcaaagcttg tatttgcgtt gcttataaac
       61 gtctttatgg gcttccactt cgtgccgtca tatacatcga tcatgtatcc gtctgccgaa
      121 gtgttcttat cccattgaag cttgatttca ctgcttgttt tcgcaacgca cttcattcct
      181 gtcggcgtat tcggatttgt agttacgtta agataaaccg catcgctgta ttccgtcacg
      241 cttccgaacg tcttatacgc tctcattctg aacttatacg ttaagcttgc tttcagccct
      301 tttatatcaa agcttgtatt tgcgttgctt ataaacgtct ttatgggctt ccacttcgtg
      361 ccgtcatata catcgatcat gtatccgtct gccgaagtgt tcttatccca ttgaagcttg
      421 atttcactgc ttgttttcgc ggcacacttc attcccgttg tagtgtacgg ttttgtgttg
      481 acgttaaggg taacataatc acttgataag ttatctctgt acgcttttaa cctgaacgaa
      541 tatgcagtac ctgccatgag tccgcttaca ttgtacgaaa ccgtggagct gttatttatc
      601 tttgcaatct gcgtccactt tccgcccttg tactgctcta tctcataacc gctcgcgttt
      661 gcgttcttat cccacttcag cgaaacactc gatgaagtcg ccgattccgc cgcaaagcct
      721 gtaggagttg caatatcatt ttcgatctta atttccgtat aaccgagaat atagtagtat
      781 ttgtattttg aattgtaact gtaatcctgt ctgacctgag ttccgttcag atagaatttt
      841 gcttttttga gattatactc atagcagtcg gcattgatgt atatatagca ccagtacgtc
      901 ttgcccttct caaatacagt cggtgtcagc cactcttcat cgtcatacca gtcaatactt
      961 ataaggctca tatgctcagg aagattatgt gagatgaaac tctttgccgg agttcctcct
     1021 gccttcggag gtgttatctg tacatatata tcctgatcat attcttcaaa cggcaccctc
     1081 agactgttaa tcactaccgt tccgtcattg gaaaccgtga acagttcctt gtcttcggat
     1141 gtcagccaga atccttctct gtaagtatca agcctgcatc ggaatatata accgggagaa
     1201 agcgcatatc ccgacttagg cgttaccttg aattcgacca cataacagtt atctactata
     1261 agctcgtcat cgtcgtaaac ctcttcgtta tattcccatg tgtttaaaag cggcgtgaca
     1321 agagttaccg aatatttatc aggttccgat gaagtaagtt gcagatcgtt tacctttctg
     1381 taaggcagga agttgctgan nnnnnnnnnt ctctaccccg accgtcctga ttccttcatc
     1441 gcaaacaaga acgctcttaa atgcaatctc gttaaggccg atgtaaatat ggtctgccgc
     1501 ttgccattca tttgcgggat cctcaaacct gcgcatcttt actgtgttgg ttgtatacgc
     1561 tgcaacgtat ccgctgttca gctttacttt tataaatatg ttgtatttga agcctttgct
     1621 cagctgacga tcgtttacta tattgtttcc gtaaacatca cggatattgt ttatcgtcag
     1681 actgtatttt tcgggttcac ccgacgcaac gctgacatcg cttaacgtcg aacctccgtg
     1741 aaagcccgac acgaccgcct tgatttccgt tacagcttca aagtcataat caattattat
     1801 tatctcattg attctcagca cattgccgta atttataaca gccttataca gcattgtgca
     1861 tttttccgta acctccggcg gcgtatacga cagatcgttt atagcttctg gaattcctgt
     1921 gaacgagccg aaattcttgg atacatacca atctaccgtt tttatcggat cgcccgtacc
     1981 gtacggattg ggagcctgag tgagcaggtc aatcttacct acgctgttgc tgttgacggt
     2041 aaaacgtgtg cgcttgttat tgctcagcac ataattattt ctgtcggtct tcagcctgca
     2101 gatttcgtct gcgcctcttg cggccatagc cttcttttcg tcaatgccgg cctgcggtgc
     2161 ggtaagcaga gtcgtgtact ctaccttttc tgcagaattt gtgattgtac ctgctttaga
     2221 agtaagcacc gccttctcgc caggataata tacctctata tcatttgggc taagcggcat
     2281 tgttccgccc aggagcgtta tgttgtaata ctttttctgt aacgcctcgt cacttgtaac
     2341 acggacaaaa tacggatcgg aaattatcgt tcccgtttta tcggtcactc tgcccttgct
     2401 cttgtttgtg atggttccgc ccgtcataac cagcgatgcg taataatcac aaccatctat
     2461 cgaggagagc gctatactgg aggtagttac tatagtacct ccgtttatca gagcggtaga
     2521 attaagcgtt ctttgtttgc cggcaaaacc gttatccagc tttatcggag taccgatctg
     2581 tgcatcaaaa taaccgtcgt tgattacaag atccgacgca ctcagcttga tcatttcaca
     2641 atacatcgca ttggtacccg tgtagcagaa tgtacctccg tttataacga tcgtacttct
     2701 gttctcagcc aaaaacagat tctgggctac atccttggca tagaaataca ttttacccgt
     2761 tcccaccgaa tcctcaatgg tcagcttact gtattcgacg ctcaacagat gatccttcga
     2821 gtcaatactg ttacctgtct ttttaagcgt atatccggca agatccagcg tcttgtcact
     2881 ttctataggc ggacacaatc tcgtcagata gttaggttta tattcctcag ggtcgcagtc
     2941 aatatctgcg ccaagcctta tcttagactc ctcactgccg ttgtatgcca atctcagctg
     3001 ccggtaacta cggacgataa ggtatccgtc acttgaatac tctgtgcttt caccgtcggt
     3061 gcctgcggca ctgataggta tgattgtcag cggcagtacg gttatcataa tagcaagtgc
     3121 cgcaaacaaa gccgcaatgc gtttaaattt tattttcata atcctttacc tcctcattat
     3181 aattttcaaa gttcatataa gcccactgct ctttattata ttttacaccg ctttcagaag
     3241 aatgacatta cccagatggg taaatcgtgc aatttatctt gatcccgcat tacattttac
     3301 attgccggca atgcccgggt tcggtaatta tacagctttt ccgcaacaaa actccggtcg
     3361 cagcttcctc ttacggtaca aaaatcggca gacacatttt cagctcctgc cgtatcaatt
     3421 attcttttgt ccaacacagt aacgctgcat ttaagctctg tcgccgcgat gcacatcatc
     3481 cttgaaaaca ccgtttccca tatggtgcat tgccgaaagg cagtcacagc tttttgccat
     3541 gaccgcccta tcatcttctt tatcgctttg tgtgaccccg ccgttgcgct a
//
LOCUS       DAWAOG010000175         3580 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3323_length_3580_cov_1.920284, whole genome shotgun sequence.
ACCESSION   DAWAOG010000175 DAWAOG010000000
VERSION     DAWAOG010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3323_length_3580_cov_1.920284"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1841..1850
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2972..2981
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1089 a          673 c          848 g          950 t
ORIGIN      
        1 tttttgcact ggtactgggt gttgttattt atgccgctgt tgccggtggc ttttccacat
       61 tcccggaaaa tgtgctttct actgtaacaa gaccttttgt tacagtttcc aacgctgtat
      121 cgcagtgggt agaggatacg cttgataagt ttgcaaacgc agacagatac aagaccgaga
      181 atgaagagct taaggataag cttaacgaac agtacaagca gataatcgac cttgagaacg
      241 taaagcatga aaatgatcag ctgaaggaaa tactgaaaat aaaagaggaa aatgaggact
      301 ttgaattttc cgcaccatgc aatataatcg caagaaattc caacgatgca tatgcaggct
      361 ttactataga ccgtggttca gacgatggta tagcacttta tgacccggtt atgacatcca
      421 acggactcat cggtatggtt tgtgagatag ctcctaatta ttcaaaggtt gcgactgttt
      481 tatccgatga aatcaacgta ggcgttatga cattcgacag taaggtaaca ggcatactcg
      541 acaacgatat aaagaatgcg aatgacggca attgtcttat gagctatatc acaaaggaca
      601 gcggcatcaa ggaaggcgag atagtgaagt ctgttgcagg cgtagtgttt cccggcaatc
      661 ttcttatcgg tgtcgttaca aaggtatatg acgatgaaaa cggtttatct gttcatgctg
      721 tgataaagcc ggcagtagat gtccgtaccg ttacagactg tgtcgtgatc accgacttta
      781 aagggcaggg tgtagttgac tgatgaaatt cagaattata agtcgtacgg caatgacgcg
      841 aaaagagata tttatgagtg tcacaaagtg ggtgttatac tcacttgtgc ttgcattatc
      901 gtatacaatt caggtcacga tgccgtttac cgtatggcaa ccgtatctga cgctcacagt
      961 tgctgtatct gtttcttttt ttgaaaacga actcagcgga gttgtgtttg cggcttttgc
     1021 gggaatgatg catgaccttg caatgggctc gctgttcggg tttacctcaa tatggcttat
     1081 gccgtgttgc ttgtttgtca cactgtttgc ggtaaatctt atccaccgga atatcctgaa
     1141 ttttctatgg atgaactttt ccgctttgat aattgttgaa gcggcggagc ttcttttcaa
     1201 atatgtgatt tggagaaatc cggatataaa tgttgtattg cttgattatg tcctgcctgc
     1261 tatgatatcg acagtgcttt tatcaacacc gatttacctt cttataagac ttataaacag
     1321 aaaactggga gctgaaaata cgaccgagga tattcttacc gcttttgaag atgccgaaga
     1381 cagcgatgac aatgtgagat attaacggag gaaagagtga ctaaattaag cagacagata
     1441 agaaatatgg tgtttgttct gatagtagtg gttttgtcgt ttgcctgtac gatcgaatta
     1501 ttacagatac agatcgttga cggcgattat tacaaaggtc agaccacaaa cacatacact
     1561 gcaaaccaga ctatccaggc ggcgagaggt cagatattta caaaagacgg aaaagtactg
     1621 aacaccaata agcttgtata caagattata attcaaaagg cttttttcga agccggtact
     1681 gagaatgacg ttatagcgaa aacgcttaaa atacttcaaa ataataaaga ggaatggata
     1741 gaaactatac cgataagcaa aactgctccg tatgagttta ccggtgatga cgacgagata
     1801 gatgcgctca gatcaaagct tgagctggga gtttatgcta nnnnnnnnnn caaatacggt
     1861 caggaaatgc gcagatatat agcaggtgtg cgctatgaaa tgctcatacg cgacttttca
     1921 tatcagaacc gctatacaat ggccgaagat gtttctgtgc agacagtaat tgagcttaaa
     1981 gaacaaagct ttatgctgaa gggcatcgat atcatagaag aagctatacg ccggtatgac
     2041 ggcaaggatt ttattccgca ggtagtcggc aggatcggag caatttctcc tgatgaatat
     2101 gaagaacttt ccgacaaagg ctacagcctt aacgatacca ccggtaagtg gggcattgag
     2161 agcgcaatgg agagcacact ccgcggtact aacggtatcc gctctataac aagagattct
     2221 tcgggaatgg tcgtatcaga tgaaataacc aaggcggcta ctgcgggaaa ttcggtaaaa
     2281 ttgactattg aaagtgattt tcagaatgat gttcaggcaa tactcgaaaa tcatatcaaa
     2341 tggcttcact ataataatga tccgacgcga ggaaacaaag tcgatgcggg cggtgttgtt
     2401 gtgcttgatg cgaaaaccgg cgcggtgctt gcgatggcaa actacccaaa ttacgatatc
     2461 aataagtata tcgaccttat aaataatccc gttgaaccta atccgacata caacaggtgc
     2521 atttacgggc tttatcgtcc gggctcggca ttcaagacaa taaccgcaac agcggcactt
     2581 gtatcgggta tagcaaacga aaatacagaa gtgtactgcg gcggcatcta cacatattac
     2641 gatgattatc agcccggctg tataatgggg tttgtcggta atgtaacggt gaggacggcg
     2701 ctcagacatt cgtgcaatat ttatttctat gaccttgcaa aggtagccgg ctggttcggc
     2761 gtaggtcagg atcttaacct tgagatcggc ggcgcgaccg gaaatatgag taccccggaa
     2821 tatatagagt cccttggcgg tacatggaca cccggttcta caattcaggc gggtataggt
     2881 cagcttgaaa caacggtaac accactcaat ctggcggtga ttgcgttgac actcgcaaat
     2941 gacggaacac gctatcagcc gtatcttgta gnnnnnnnnn ncaagctatg acggcagtaa
     3001 aacgatcaag aaaacagagc ctactgttgc gtataaaata gatgccgaca agcatatatt
     3061 tcaggtggtg cgtgaaggta tgataatggt cgccgatgat gtacagtggc ctatgggctc
     3121 aggtataaca cagcttgccg acctgccata cggagtggcg ataaagacag gtactccgca
     3181 ggttacggaa gatactttca attctactat cctgggatat tatccggctg aggatcctca
     3241 gatatgtttt ggaattgtat tggaaaaagg cgagttctca agattgatgg tacgaaatat
     3301 aatagaatcg tacttctatg acaactatca gcctgtgacc aatgaaaagg gcgatataat
     3361 tcttccttgg ggcggtcaga gcacaaacac tcccggcaga cctgccagag gagagttgac
     3421 tgagaataac aaaaatgata cgtcaaacag ttcagaaaac gaatgacgtt ttgtgcagtt
     3481 tgcataaaaa agtaagctaa aaacatattg aattaacaaa atttgatttt tttcaattaa
     3541 aagactttat ttatgcgcgt ttttatggta caattaaaac
//
LOCUS       DAWAOG010000176         3578 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3327_length_3578_cov_1.869429, whole genome shotgun sequence.
ACCESSION   DAWAOG010000176 DAWAOG010000000
VERSION     DAWAOG010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3327_length_3578_cov_1.869429"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1024..1033
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1053 a          748 c          739 g         1028 t
ORIGIN      
        1 aaaaagcaca gacaggcatt gactggttgc atgaactggc tgatagacag ggatatgcgg
       61 ctgcgggtat aaatctcggt attctttatt ttacaggtga atgggtagaa gccgatgtcg
      121 aaaaagcact gtactacctt gaaaaaatca agcagataaa taaatcagag cccaactatt
      181 ctataggctg ttattattat gctctcatgc tgttctataa ggataaaaac tgtgaacagg
      241 ctgtctacta tatgaaagag gctgccgaga gaggtatggt agatgcactg cgcgatcttg
      301 ccgtaatgta tcagttcgga ctgggagtaa aaaaggatat tatgcacgcc aacgaactgt
      361 acgcaaaggc ggctctcggc ggagattatt attctgaaaa ctggcgggat tatacaaaga
      421 agtatctcaa ttcgctcagg acaatgagca aggtttatgc tgatgaaagc gaagatgatg
      481 atctgtatat cagcgaaaca aaagagcttg atgaaaactg tattaacgga ataggtctta
      541 attcgtatgt ttttaattat cctatagaag aatctaaaaa tcttcccgaa gacaggataa
      601 agaaattaaa acgtaaatat gcaggtctag aaagaaatct tatatcatct gctcaggtgt
      661 ctttaaatca gtccaacgtt ggttcgatat ttatgaactt tacaaaattc agacaggttc
      721 aaggtcacgg acacgcgttt gaataagcaa atcatgttga agatttgttc cacttcaaag
      781 ccgcatcatg gaaaggcgga gataacgtaa aaggtggagc agatcgagtt gtaggtcttt
      841 ttaaacagac cgaaattcag tctaaatgct atagagatgc taaaatgcta tataatcata
      901 cgtttgataa aaacggaaat ccaatatatg ttggtgaaaa cggcaaaccg atggtaattg
      961 aaactacttc agacacgtat gatgaatacc ggaaaatggt tatagataat aaaggagaag
     1021 aatnnnnnnn nnnaagtaaa tactcaaaca agcaagtaga aaatataggt aaattcggaa
     1081 ctatagagtc tttgaaagtc gatgcaaagc tcggcatcgt gacagctacg agaggtgcga
     1141 tgatttcagc tgtagttaca tttagcgtat ctaagataaa cggtgcagat aatgaagaag
     1201 cggtaaagtc cacactccgg gctacagcta aagctttcgg aactacattt gctattacaa
     1261 tgatttcaag ccaattgctt aaagctacgc cggtagtaaa tgcacttgag aatagtcata
     1321 tgttaaataa gatggcaaat tcaaaatcac tacaaaaagt agtcagtaaa agtacaggca
     1381 ctgcggcatc tgcatcagag gctaaaaact ttttgaaggg aagtgtagtg accggtgcgg
     1441 taactattgc tgtcttatca tcggcagata tattgcgtat aataagcggc agaatatcca
     1501 aagaacaatt atttaagaat gtcgccgtaa ccactgcaag cgttgcggca ggcagtgcag
     1561 gatttgcggt cggcgcagcc tgtggttcgg taattcctgt tgtgggtacg tttatcggcg
     1621 gctttatcgg ttcggctgtt gcgtcttctg ttgcaagcaa ggctgtaaaa tgcacattag
     1681 atgtatttat tgagaatgat ggcgataaaa tgctggaaat tctgaacggt gaactgggaa
     1741 tgcttgcaga agagtatatg cttgcagaag atgaactaaa tctggtgata gatgacctca
     1801 gaggctctga tctgctgaca gacagcggtc tgcgcgacat attccagtca agtgacagaa
     1861 aggtcttgtg ccgtaataaa ctcactccta tggttgaaga gatatgtatg ctccgcaatt
     1921 atatcgcatc gccatctgaa gaagatatag caaaaggctt tgaggacttt agcaatgacg
     1981 aagatgtaca gcagtatttt tctacagaag ctgtacctgc aatgtaaaaa tacatagctg
     2041 tgacacatta tataaactgc agaaaaacag ataaaacttc aaaatgcagt gtcactattc
     2101 cctgctctgt cggttttgca tatacgcaag ttcatttgga tcaagtgtac ttgcttctga
     2161 agcgaagcac tgtacaggtc aacagccaca cagaaccgtt gcatcgcgca acggcactgt
     2221 gtggctgttt ttatgcgtcc ccggcagtga ccttatgcgg ctgactgcaa gcgtcaatgc
     2281 tcagaccggc ggcactagcc ctggcactgt caaacaagct cttcaaattt agcttttcag
     2341 cgcattctta tatccgatta tcccagctta tccgcgcaca tttttctcac agtataattg
     2401 ccgctatccg aaatatcggc atatatttca tcgccgtctg aaaattcacc ttttattata
     2461 ccgtcggata tacggtccat aacattctta tcgatcagct ttgctatagg tcgcgcacct
     2521 tcatgcattt tttccgcatc agcgcatatt ctgtctgaca aacgctcact ccacgacagc
     2581 gacactccct gccgactaca cctctttgcg agctttgcaa gcatattgcc gcatatctgc
     2641 ttaaaatcgt tcataccaag ctcattgaat actataactt cgtctaccct gcttagaaat
     2701 tcaggtcgga aatattcctt gagttcattt cttattagct ctgatattac tgtttgtctt
     2761 tgctgagtgc agaagcctaa cttgtttttg cctccgctta taagtctgct tcctatattt
     2821 ccggtcatta taataaccgt gtttgtgaaa tccgccgttc ttcctgcaga atcggtcaga
     2881 tgtccgtcat caagcacctg taacaaaata tcaaatatat cgggatgggc tttttctatt
     2941 tcatcaaata atacaacgct gtagggcttg cctcttactc tgtcaatcag ctttcctccg
     3001 tcctcatacc ccacatatcc cgcaggagca cctataagct ttgatactga gtgcttttcg
     3061 ctgtactctg acatatcaaa acgtatcaaa gccttttcat caccgaataa agctcccgcc
     3121 agctccttac agcacttggt cttgcctact ccgcttgtac caaggaaaat aaatgagcct
     3181 ataggacgat gttcatcgga aagcccggag cgacctcttc ttatttgcaa cagctgatat
     3241 cgcctcagtc tgacctgtta tcctttctga cagtattctt tcaagctcaa caagcttttt
     3301 agcgttgtcg ccatcatagt ctaatggtat acctgtataa cttgtcacaa catcggctat
     3361 atctttctct gtcaatatag cgatcgggct gtcagcctgt acacctgccg caactgccct
     3421 ttcctcttca cggagtctta acgcaagtgc aaaatcctta tcatttattg cttttatttt
     3481 tctctcagac agctctttta atctgttatt ccgatttgcc gtttctcctt ttattgtttg
     3541 tgaagctctc acttttgccg aagcttcatc tataagat
//
LOCUS       DAWAOG010000177         3577 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3329_length_3577_cov_2.335605, whole genome shotgun sequence.
ACCESSION   DAWAOG010000177 DAWAOG010000000
VERSION     DAWAOG010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3329_length_3577_cov_2.335605"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          846 a          913 c          755 g         1063 t
ORIGIN      
        1 aaaaagtgag ccgagcgcac tatctttgcg cttcgactca ctttttttct ggttgcgtca
       61 caacccctga tatatccgaa ttaaatcaat tcaatcaaac actgctgtgc ttcttaacgt
      121 agtctacaac ctcgtcaaca gttgtgaatg ccatgcttac gcaggtatct gcgcaaccat
      181 aatagatagc aattctgcct gtatcggcat cgcagagtgc tgcgcagggg aatgtaacgt
      241 tctgaacgtc gcctacgcac tcatagtact ctctggggtt gaggaggtac tcagcgcaac
      301 gatacttaac cttccagggc ttgtcaatgt caaggatagc agctgagaag ctgtatacat
      361 aaccgttgca gctctgaaga acgccgtggt agaataccag ccagccttcg cttgtttcta
      421 tcgggatagg acctgcaccg atcttgagcg attcccaacc cttcaaggga gccataacgt
      481 gtctgtgctc gccccagtac ttcatatcgg gtgactgtta aagggaagga atgcgttttc
      541 gcactgttgg acctccttga agtcctttgt ccagcccaca ccgatagtag gcttccagcc
      601 gtaagcatta caccatgtga tccagaatct gtcttcgatc catacacatc tggggtcata
      661 gccgtactgc caggggttag cttctgctgt ttcgggatcg tcgtttatcc attcaacctt
      721 ttcctcgttg atgttccagt gtacgccgtc ctcagagaaa cctgcatgga tagccatatt
      781 tctttccttg tcgtcaacac ggaaaacacc tgcgaacttg cccttttcgc tctcaaaagg
      841 aacaactgca ctgttgaaga tcgagttgct tgtaggaaga aggtcacggg ggattatggg
      901 gttgttatct gcgcgccaga taacgtcctt gcaacctgcg ggacgatcct gccaagggat
      961 gttcggtaaa gatgtaccgt tgattacttt tacgttcata cttgattctc cttgataatg
     1021 tcatatttcc gttaagaagc gttaaacgct ctttgtatta agtatatcat agtcattctt
     1081 taaatgcaag agtttttgtt aaaatttttt acttaatttt tggactaata aactaaaaaa
     1141 caagtaattc cgggcgttat ccccaccccg gacagcctcc ggtctatgcc gccatgaaga
     1201 tatcaaagct gataaaagga cgcttgcggt cagccgcata agcaacaccc gacagtccgg
     1261 gactatttgc gcaattccca ataatacttc ctgttcgccg ccggaaaatc actcatgacc
     1321 ccgtgctcgg gatcgtaaaa agtgccgtcc gcatacagtg accagtgcca gcaccgcggc
     1381 gtttcaagac tgagcagaca aagcggcggc agctgttcct ttctctgtac cgcaatgcgt
     1441 tcatctgaaa tataaacgca gtgctctctg aatgctcttt tcatctcagc aagcgtagtt
     1501 tcacggtcat tgtcaagaaa ttccactatc tcgtcaaccg tctttcccag taccattgca
     1561 agcacagcct gtccgcactg taaatcggtc ggctcgtgga tatattcaat atcggtcata
     1621 tattttcatc gctctccggc agtataatgc ttattgcttc atgcaatacc tctatttcgg
     1681 tatcggttct gctctccgcc tcttcgccgt caagcgacca cggcagagcc tcctcgcaat
     1741 ggatatcaag tcgctttgta tggaagaaat caagaaattc gttattatat tcaccgttta
     1801 gaagtgcctt tgccataagg cttatctgca caggcgtttt cggacagctc acaagcatta
     1861 cctcaaatac gccgtcgctt aaatccacaa gccgcttatc aagcttgatt attccgccga
     1921 ctgaggtcgc attgcaggcc gcaccgaaga tataatcccc ctcgtgcagt attccctccg
     1981 cgtcataaac gctcatgtga tacggcttta tctccgcaag ctcctttata ccgctgagta
     2041 tatacgcaaa atgaccgaaa gtatttttaa gatgttgcgg tgtaccgtaa gagctttgtg
     2101 tgaatgcgcc gaatgaggcg atatacataa attcctgttc gttaaatctg cccacatcaa
     2161 aagctttcgg ccgtcctgta actatgtctc cggccgccgt gagcgtctgt gatgacaagc
     2221 cgatcccgta cgcaaagtca tttgtagtgc ctgacgggat ataaccgatt ttcgtcctgt
     2281 gaccgcccga tatcataccg ttcactgttt cgctgagcgt tccgtcaccg ccgcagcaga
     2341 tgatcacatc atattcactg ccgtatattc ttgcagtttc ggcggcatca ccgcgtcttt
     2401 cggtaacgtg tacggttgtt gtatatccgc cattttcaag gcaactgatt atattcacaa
     2461 gctcgcctgt tattcgtttc tttcccgaaa cgggatttac tatcagtaag gctttcattt
     2521 taaaattcct ccgatattaa atttcttata ttatattaca cgatttttaa ctgacagtca
     2581 acggtaacgc caaaataccg cacagcattt ttgcctgccg tgcggtaaga tatcattttc
     2641 agtcctttat tataggttcg gtcgaatttc ccggtgtgtt attctcccgc gcatatactg
     2701 cttccttcag ctttacgaag ccgttataca agctttcctc aggcaacgcc ttaagcgccg
     2761 ctatctgctc agccgtaaga tatgacgcta atttctgagc ggtttctatc gaaatatcct
     2821 tgctctgcaa agagtcgata tcgcttatac tcagcttttc ggcaagcgtt tcaaacgcgg
     2881 tgtttgcttc atcgttatcg ctgaacacta tctgatttac cgtctgctta gaatcgctct
     2941 taggcatcat cgtaccgttt gaattgatta tataatcgcc ctcaagcaac agttcgctca
     3001 ctgtgcaaag ctcaaatccc tgctccttaa gacttttgag cacagtcggc agtgcctgtg
     3061 cagtattttc aaggtcgttg tggaaaagca gtatcgaacc gcccgaaata ttcttcgttg
     3121 tacgctctat tatagcttcg ggtgtcggct tatcccagtc tatgctgtcc ttgttccact
     3181 gtataacgct cattcccata ccgaaaagcg tagttaccgc cttgtcgtca tattctccgt
     3241 agggtgcgcg gtaaagcgtg ggcgcattgc ccgttatcat ctttatcttg cgtgagcatt
     3301 cctttgtgtc ggcgataaga tcattcacat tcataccctt tatatgcggg tgcttgtcgc
     3361 tgtggtttgc tatctcatga cctgcgtcac tgagcttctt tatatcatcg gcatacttat
     3421 ccgcaaattc gcctgtcaca aagaaggtag ccttcgcatc ggcttcggca agtattgaga
     3481 tcagcttgtc ggtgttgctg tttccccatg cgcagtcaaa tgtaatgctg attttgttgt
     3541 cagtgcgttt tacattatat ataggcagct tgatatt
//
LOCUS       DAWAOG010000178         3572 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3338_length_3572_cov_2.552459, whole genome shotgun sequence.
ACCESSION   DAWAOG010000178 DAWAOG010000000
VERSION     DAWAOG010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3338_length_3572_cov_2.552459"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3187..3196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          823 a          955 c          726 g         1058 t
ORIGIN      
        1 cgcaagcgtt tcctttatag gtgcggcatt tgagaaacgg cggtagcctc tcttatgaag
       61 cccctcgccc gatgtatcga gcgtcatagt gacgttatcc ttcattattg agaagcggaa
      121 ctgaaacagc gcctccgttt cctgaaagta gccgatttta tatacgctct tcattcgttc
      181 aaccatagcc ttcttgatta tcttctggca ggcaggaacg cttgtaagct ttgaatttaa
      241 gctgaagccc tttatcggga acttatcgaa cttgccgaca tatttttcta tcggaattgc
      301 ctttaccccc tgaaacagct cctcaaaggt cctcgccctg aactctgaca gcaatattcc
      361 cacgcgctcc gcagtcgaca ggcacatatt ggctcttata agcacacgct catctcccgt
      421 gaacgtaaca cgtccgtccg atacggctat atcctcgccg cctattttgc gcacctcaaa
      481 cgagagcgtc ttttccagcc cgaaatggca cggtgccgta agtctgaatg tcatagaata
      541 tcctttcatc aaaacagcgg cttttgtata gccgctgtaa tctgttttat atcagtttga
      601 gcttgtatcg ctcgtgctgt cgtcctgctt tgtgtaccat tcgggcacaa gcagtgtttc
      661 accgccgtgc tttttcacaa actcgccgtc cttatggctc gaattacaag tctttggcaa
      721 tgtatccgcc ttataatagc ccaccgccgt gctcggacac tttgatgtgg cgagcagtcc
      781 ggtctcggtg cagtagttct tttctactac attcggatcc ttggtgaatg tctttgtaga
      841 ctcgtcgttc acgatgttca ccataaagtt gtgccatacc tgtgcgagat aaacatagtt
      901 cgtattgccg atgttcttat tgtcatcata tcccagtctg ataacgccgc agtaatcggc
      961 tgtaagaccc gcaaacgcca gtacacgttc atcgtttgaa gtacccgtct tgccgacaac
     1021 ctcaattccg tcaattctcg catatctacc tgaaccgttt gtggtatcgg taaccgcctt
     1081 gagcaatctg ttagtgatat aagcactgtc actgtccatt accctgtcgc cgagcggttc
     1141 ctgctcaata acgacattat cgttgtagtc tgtgatgagg ttgtatagct ttggagtata
     1201 atacataccg cccgagccga atatctgata tgcacccgca agctcaagca gcgtcatacc
     1261 ctgtgtcagc tgtccgagcg cgataggcga ataagcctta tcgttcgtgt aatcaagtgt
     1321 cgttacattc agcttatcgg taaggaagtt gaagcaggta tcaattccga gcacctgcgc
     1381 cattcttaca gccgtagtgt tcttagactg ctgtaccgca taccacgcag gtatatatcc
     1441 gcccgagccg tagtcgccct caaagttact cggccagctt gttccgtccg gaagtgtgat
     1501 acatctgtcg aatatcattg acgaatagct gatgatatcc ttgtctatcg cctgaccgta
     1561 aaccgagata ggcttcattg tcgaaccgat aggaagtatc gcctgtgtcg ctctgttgaa
     1621 gcagccgtcg ccgggcttat ctccgatgcc gcccgcaagc gccacgacat tacccctgta
     1681 gtctgtcagt acaaacgccg cctgtgcaca attctcctct attgctgtct tatcatagct
     1741 gtagaagaag ttgttggggt ctttgaagaa ctcttcaagc ttatcctgga tatccatatc
     1801 catattcaga tatatcttgt agccgccctt gtaaaggtca gcctttgcct cggagtaagt
     1861 catacccttg gcttttccga tatcctcgat gacctgttct atagccgcgt caacatacca
     1921 gttctgcgat atctcatact cattccagcg gtaagcctcg taagtatcgt tatcgtcata
     1981 ctgatcgctg ttgtcgtcct gatttgcatt gtttccgtaa tattcatcat atctcggatc
     2041 tttatagccg taagcgtcac cctcaacgat aagtctgaac ttgacctgtt cgaccttagc
     2101 cgcttcatac tcatcggtcg tgataaggcc ctgctcgtac atacatttca gcgcgtacag
     2161 ctgtctttcc tttgatcttt gaagattagc atacggatta agcacactcg gcgaacgcgt
     2221 catacctgca agtatcgcgc tttcggcgat agtcagctga tctaccgttt ttccgaaata
     2281 atagtaagcc gccgaaccta caccgtaaac cgtaccgccg agcggaacac ggttaagata
     2341 tgcctccagt atatcgattt tcgtgtattg ctgttcaagg ttcagcgcac ggaaaatctc
     2401 tcttatctta cgctcgggtg tcaccctgtc gtcgcctgtg atattcttta caagctgttg
     2461 ggttatggtc gaaccgccct cgccgtagcc gttgaacagg gtcgatatgg tgacgcgcac
     2521 cgttctcttc cagtcaacgc cgtcatgctc atagaaacgc ttgtcctcta ccgccgtgaa
     2581 cgcgtcctga gtgtgcttgg gtatctgctc catatctacc catatacggt tctcgtcacc
     2641 cgacagacgt tttatctcca cctgttcacc cgcgctgttc tttgcgtaaa tgaagctggt
     2701 gtagctcagg tcaacgttct taagatcaac gtcaaatgcg ctgtccgcaa actgcatgat
     2761 gtagaccgtc agcgtcaccg ctactataca gcaggtgatg atcactatca taaacaagct
     2821 gagaagtgtc gttccgataa cgccgaatac atgacgtaca gtgcttacag ccttattctg
     2881 ctttttcttc ttttttttca tctttgtctt agtgccggca gaaagctctt cttcaagctc
     2941 tgcatcggca ttaagatatt gagtgttatt tttgctcata tttacgtcct tttaaggtgt
     3001 gtcgttcatt ttattattgc cgtttcaggc aaagttatgt tctctgtaac agagtatacc
     3061 acgttgtaag tataacacaa ttcgtatcaa atgttaagtc ttttgtgccg aattttcata
     3121 aaatattcaa aatagctgtc acagccgccc gaataatgta aattttcact aagcaacagc
     3181 atagccnnnn nnnnnntcac aaacaccgcc aaccccgaac tatcccatac accacaaaaa
     3241 accgggcatc agctttcacg ccaatgcccg attaaactat taacttttcc taagcgctgt
     3301 tattttgccg ctgtcttccc actgacacta ccataattac cgtaaagcgc cgtctttccg
     3361 ctcatgtgat aagcgcaaac cctgaactta taagcagtgt tcttagcaag tcccgctttt
     3421 ctgtaagtat tagtgctgtt cttagttatc ttagcaactc ttacccactt tccgcccttg
     3481 tacatttcaa caatataccc ttgcgcggaa gcgttcttag cccagttcac tctgagtgcg
     3541 tccttagcct taccgccgat cttaacgccc tt
//
LOCUS       DAWAOG010000179         3570 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3341_length_3570_cov_2.170413, whole genome shotgun sequence.
ACCESSION   DAWAOG010000179 DAWAOG010000000
VERSION     DAWAOG010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3341_length_3570_cov_2.170413"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    231..240
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    717..726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          885 a          824 c          773 g         1068 t
ORIGIN      
        1 tggcgtgtgg gttcaagtcc catttcccgc accagtgtaa aggacattcg tttttgagtg
       61 tcctttattt tttatatcta agcaaatcag caaatcacta tcattaagga caagcgcgcc
      121 ggaatcgcac gaaatctttg attttgtgga agcggaactt gttccgcagt cagaccaggc
      181 aagccccagg tgcctcagtc agtattggcg tgtgggttca agtcccattt nnnnnnnnnn
      241 aatgtatgta acttttcact gccgggatat ttgcagtcaa cagtataatt gttgctgtat
      301 gtccattttg aacggttgga attaagtcct ccgtgctttg ttatccgttt gaatatcacc
      361 cgtatctgtt tttttctgcc tttatagaat ataccgagag aaataagcga caaagctgaa
      421 agtgcaatgc agataactat aactgtctgt aaaagttgca gtacattatc catgtttact
      481 tgtcgagcac agccatgtaa gcctttacta tctcaacgca cttatcgtag ctcatactgc
      541 ttgtatcaag gcagaggtta taatttcttg cgtccgtcca gtcacgacct gtgtagtggc
      601 ggtaatactc actgcgtccc ttgtctttct tttcgataag cttcatagcg tccttgccgt
      661 caagtccgta tctgtcgtaa acatttctga tggcggtttc ggtatcggaa tagatgnnnn
      721 nnnnnncttg cgacctttta acacaaaatc cgcacatctg cctactatca cggcagattt
      781 tccgctgtcg gcaatatcgg ttattatctt tgcggtgtaa ttgaacagat tgcggtcgga
      841 ggtgaaatcc ttatgatcgg gctctaaaag ctcaccgtca taaatctctt cgcggttgaa
      901 gatcgtgttc tttacctttt catcaactct gccgaaaagc ttctcgttga tgccgctttc
      961 gtcacttgcc attctgatta tctctttatc atagtacgga atgtcaagct ccttggcaag
     1021 catttttcct atagtcttgc cgccgcttcc cattcctctt gcgattgtaa taatacgtcc
     1081 cataagctcc tccttattct ccgagataag ccttctttac cgaagcatca tttataaggt
     1141 ctgatgcctt gcccgaaagt gagatcttac ccgtttcaag aacatatgct ctgtctgcaa
     1201 ttgaaagcgc tttctttgca ttctgttcaa ccagcagaac agtagttcct gtttctctta
     1261 tagtctggat tatatcgaaa atattgctta caaagatagg tgaaagaccc attgagggtt
     1321 cgtccattac gatgatcttg ggcttcgaca tcagcgctct gcccatagca agcatctgct
     1381 gttcgccgcc cgaaagagta cctgctatct gcgtctttct ttcggcaaga cgcgggaagc
     1441 gttcatacac catctgaata tcctttacga tgttttcctt gtccttgcgg gtgaacgcac
     1501 cgagcttaag gttatcaaga acggtgaggt tctggaaaat acgtctgccc tcgggaacgt
     1561 gagctatacc catttcgact atcttgtggg caggcgtttt ggtaagctca acaccgttga
     1621 atgttataga accgctctta gcgtggagaa gtcctgttat tgtatgtagc gtagtagtct
     1681 ttccggcacc gtttgcgccg ataagagcga taacctcgcc ctcgtttact tcaaatgata
     1741 tgcccttgag ggcggctatc ataccgtaat ttacataaag atccttgatc tcaagcattg
     1801 ccattttgac gaccccctta ttctccgaga tatgccgtga taacttcggg attgttcagt
     1861 acatcagagg ttttaccctg acataacagc tgaccgaagt taagcacaga cagcttttca
     1921 catataccgg atacgagcgt catatcatgc tcgataagca gtactgtcat attgaaatta
     1981 tcccttacaa agcggatcgt gttcataagt tcaaccgttt cgttggggtt catacctgcg
     2041 gcaggctcat caagcagtaa cagtttggga tctgtagcaa gcgctcttgc gatctccagc
     2101 tttctctggt cgccgtaggg caggttggaa gctaagaagt cagccttgtc atccagctcg
     2161 aaaacgtgta acagttcaag cgccttttta tccatatcct tttctgtcga gaagaagcgg
     2221 ggaagtctga acacaccctc tgctgtactg tatctgtagt ggttgtgtag tcctacctta
     2281 acgttatcga gtacgctcat ttccttgaac agtcttatat tctggaatgt acgcgcaatt
     2341 cctgcgcggt tgatatctct tgtgcttttg ccagttatgt tctgaccgtc aagggtgata
     2401 ataccctttg agggcttgta tacgcctgtc agcatattga acacggtagt tttacctgcg
     2461 ccgttaggac cgataagccc gtaaagctca cctttttcga ttgaaacgtt aaactcatca
     2521 acggctctca gaccgccgaa agagatgcct aaattcttta cttcaagaag cgccatatta
     2581 tttgacctcc ttagtttcgc gcttgaaaat cttaagaacc atactcttca gttttccgaa
     2641 gttcgttact ataaatgttc ttgctttttc gtttgatgtg agtatcatca taacaataag
     2701 aacgattgag tagatgagca ttctgtagct gtttatgcct cttaatacct cgggaaggag
     2761 cgtaaggata acagccgcga tcacgctgcc tctgatattg ccgataccgc cgagcacgac
     2821 gaatacgagt atcagtatcg aaaggttata gtcgaacttt gaagcctgta atgtagcgta
     2881 attatgtgag tacagagcac ctgccatacc tgcaaggaat gcagtaagag cgaaagttat
     2941 cagcttgtac tttgttatgt taagacctac agactctgcg gcaattgagt tgtcgcggat
     3001 agcctttacc gctctgcctg ttcttgaatt gatgaagttg tacgaaacga taagtgttat
     3061 catcaggagt atgaagccta ctacgaatga tgaatcattc ggaacgccct ttacgccgag
     3121 cgcaccgcgg attatttccg tgccgtctgc cgcaaggttc atatctgcct gattgagtgc
     3181 aatatggagt ccgttgctgt caacgccgac ataaagtgcg gcaaatacat tcttgattat
     3241 ctcgccgaat gcaagtgtta cgatcgcaag atagtcgccg ttgagtctta atacgggtat
     3301 tcctatcagt atgccgaata tagcggcaaa gataccgcct acaagtattg caagcgggaa
     3361 gcgtacccac tcgacaagta ttgtttcctt ggttgctatt gagaaaagtg cacttgcata
     3421 tgcaccaacg cacatgaagc ctgcctgtcc taagctcagc tcaccgagta tacctaccgt
     3481 gaggttgagt gatactgcaa gtattgagta aacacacagc ggaacaagaa gaccgtctat
     3541 ctggcttgaa acgtttcctg ttgacaaaag
//
LOCUS       DAWAOG010000180         3568 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3345_length_3568_cov_2.126103, whole genome shotgun sequence.
ACCESSION   DAWAOG010000180 DAWAOG010000000
VERSION     DAWAOG010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3345_length_3568_cov_2.126103"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1163..1172
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1027 a          708 c          918 g          905 t
ORIGIN      
        1 gagcacagga aagtctgtcg gcggctgtta atgcaggcgc cgatgcggtt tatttgggag
       61 aagaatattt ctccgcaaga aaaaatgcag ataattttac cgccgaacag ctttgtgaag
      121 cggtacgctt ttgtcattac agcggtgtca aggtatatgt tacgttgaat acacttgtgt
      181 ttgacaggga gatacctctg cttgcaaaag caatagagaa ctgcgccaag gctgatgttg
      241 acggctttat cgtgcaggat atgggggttg caaggcttgc tcggcagatt gtgcccgaac
      301 tgcccttaca cgcgtctacc cagatgactg taaattcgcc ggagggcgcg attatggcga
      361 aaaaactcgg ttttacccgt gtggttctag gcagagagct ctcctatgag cagataaaag
      421 caattgtcga cagttgcgat atcgaaaccg aactgttcgt tcacggcgca ctatgtgtct
      481 gtcttagcgg tcagtgctat atgagcagtg ttctgggcgg cagaagcggc aacagaggtc
      541 tttgtgcaca gccgtgccgc cttgacttta caagcggcga caggcataat gcgctgtcgc
      601 tgaaggactt gtcgcttatt gaaagattgc ctgaactaca gaagctcggt gtaacatcgt
      661 tcaaaattga gggcagaatg aaacgccccg agtacgttgc aggtgctgtg aacgcttgcc
      721 gtacagctct tgacggcgga acgcccgatc ttatttcgct taaaaacgta ttttcgagaa
      781 gcggatttac aagcggatat ttcgatgatg acttcagaaa tatgcagggt gtgcgaacaa
      841 aagacgatgt tactgcgaca acatcaaaag tactggcagg tatgaagcag tattatcata
      901 agccgtgcaa acgcttcaag gctgatatta cggtaaatgt aaaaaacgga cagcctgttt
      961 cctataagct atcagcgtgt aatgtaagtg ctgtcgctgt aggcgagatt cccgaaaaag
     1021 cggtcaacag agccgttact gccgaatatc ttgaaagtca gtgcagtaaa ctgggcggta
     1081 ccgtatattt ccccggaaat atcaccgtaa atgttgatga cgggttgtcg gtatcggccg
     1141 catacctgaa tgaactgaga agnnnnnnnn nntggaaaga agatacgtcc tgagttcaga
     1201 tgtgaggtaa gtacctcaga acagcttgaa caggcacttt tgcaggacga atattctttt
     1261 atatatgcgc ctatgcatct gctgagtgcg gatacacccg ataaattcag aattattgcc
     1321 gtaccaccgg tgttccttgc agactgcgaa aagcaagtta tggaaaatct cggtgtgctt
     1381 aaagaacagg gatatgaaca tatggctgtt catacgctct ctcacatcgg aatagccaaa
     1441 aagtgcgggc taaaaccgca cggtatgttc aggctgaata tcacaaacag atattcgctc
     1501 agagaatacg aaaagatggg gctgtctgac actgttcttt cgtttgagct gcttatgagt
     1561 gacgcggtat cgctatgtgc cgacagtgag ataaaaaccg gcatagtggg ttacggcaga
     1621 ctgccgctta tgataacaag gcgttgtccg ataagtgacg gcagaccctg cggaaagaaa
     1681 aaaagacccg actgtccgca ttatatcacc gacagacaag gtaacagtat gccttgcctg
     1741 tgtagtgaga atacagtcga gatacttaat cccgataagc tgtatcttag tgacagacag
     1801 agcgagctta caaagtttga ttttgtgttg cttaaattta ccgatgaaac ggatacagac
     1861 gcagtgcttg atatgtatct taatgatata aaaccggacg gaaagcttac aagaggactt
     1921 tattacagag gagtgcagta aatgttcata gaagttaaaa agctgaacga taaggcaaaa
     1981 ttaccttgca gagcaactgc tgacagtgca ggagctgatc tgtgtgcttg tatcaatgac
     2041 gatatagcac ttatgccagg agaacgtagg cttataccta caggtattgc tatcgctgta
     2101 cctacaggat ttggcggatt tgtttttccc agaagcggac tgtcttcaaa gttcggcgtt
     2161 tcgcttgcaa actgcgtagg tgtcattgac agcgactaca ggggcgaggt caaagtgcct
     2221 gtgataaatc attcatcgga accatatacc ataaaagccg gtgaacgtat cgcacagctt
     2281 gtgattatgc ctgtggatct gtgtgaatac ggtttcagcg atgagcttga caaaacagag
     2341 cgcggtgagg gcggtttcgg ctcaacaggc agtatgtaat gcaaattgca taaatatccg
     2401 ataaaaacgc gtgataattt atgaatattt aatttcgtat tttagttgta tgctttttca
     2461 aaaagtggta taataaatac cgaattgtaa agaagaatac cccggaggtt ttacgaatgt
     2521 tttcaaaaga tataggtatc gacttaggaa cggccaacac acttgtctat atgagaggca
     2581 agggcattat aataagagaa cccagtgttg tagctgttga tgtcaagatg gacagagtgc
     2641 gttatgtagg tcaggaagcc aaggacgtta tcggacgtac acccggctca atagtagctg
     2701 tcagacccct caaggacggc gttattgcag attttgatat gacaacaagt atgttgcagg
     2761 agtttataag aaaagctctc aagggcagag cgttcgccgg ctcaagagta agagtaatca
     2821 tttgcattcc ctcaggtgta actgcggttg agcgcagagc ggtcaaagaa gctacacaga
     2881 atgcgggcgc gaagcgcgtt tctataattg aagagcctat ggctgcggca ataggtgcgg
     2941 gacttcccgt tgcagagccc acaggcagca tgatagttga tatcggcggc ggtacaagcg
     3001 aagtggcagt tatttcactc ggcggtattg tcacttcacg ctcagtaaga gttgcaggtg
     3061 atgagttcga ttcttcgata atcaactaca tcaagaagaa gtataacctt ctcattggtg
     3121 agagaacagc agagaacata aagattgcaa taggctcggc atatccctac agtgataatg
     3181 agccttcaat ggatataaag ggcagaaacc tgctgaacgg actgcctgag aatataacga
     3241 ttacctccga agagataaga gaagctctct cagagccttt aagccacgtt attgaagcta
     3301 taaaggtcac acttgaaaag acacccccgg aacttgccgc tgatattatc gatcagggta
     3361 ttatgcttgc cggcggcggc gcgcttctta aggggcttga tctgctcatt cattcagaaa
     3421 cgggtatgcc tgtcaaggtt gctgaaagac cgcttgactg tgttgctgac ggaacaggta
     3481 aggttcttga gaatattgac aagcttattg atgttcttac agatgacgat accagatatt
     3541 gattttacga atttataatg cttatgac
//
LOCUS       DAWAOG010000181         3566 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3349_length_3566_cov_2.410994, whole genome shotgun sequence.
ACCESSION   DAWAOG010000181 DAWAOG010000000
VERSION     DAWAOG010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3349_length_3566_cov_2.410994"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1113 a          802 c          782 g          869 t
ORIGIN      
        1 ctcagcacct caggaatcaa atgcgacctg tatacagagc atttgcgcgc aaagacagaa
       61 aagcttgtaa tactcagccc tatcccctgc ggctatatgc agagctatga tgaaataaga
      121 ggtcttgtaa agagcctgct tgccgatgaa agcaaagcgg cggcagtaga cagcataagc
      181 tttgacaaaa agactatctg ctttgatccc gatacccccg taatgcgcta ttttgacaag
      241 tctgtatata acggcgaagt gtcaagaaac gtcacaatgc ttgaatatga gttctcgcag
      301 gatttcattt caaattcacg aagactcatc gatattaaaa caggactccc gctttacgca
      361 cagggtggca acatttttga agaatacggt cttgaagcaa aaaagaccta taccaagccg
      421 tcatataacg tagccgatgt tcaggagtta cgcgatgtcg acaagaagct tgactgtttc
      481 ctgacaaagc ttgacgatgt gctcaaagac tcggagaacc gtgctgttgt ttactgcgtg
      541 acaggttcga ctatactgta cctgaaaaaa gttatttctg ctctttatcc gaatatcaac
      601 ggactgttaa agatcgacag aggcgacatc ttcaacacaa gatacgacaa cttcacaaat
      661 gaaacccgcg acaacgcaag aattgttctt accgttgaca agataggttc tataaatcca
      721 caggtaaaaa acttcactca catattcaac tatgagcttc ccgacagccc cgttatattt
      781 gaacagcgtg ccgcgcggca cggcggaaag aacgaacagt caagagaatt tatcatattc
      841 tccgacaaga acaatctgct tgatcttgtt ttcacaaatt aacctatcca gctcttcaag
      901 agcatagtcg caggacttcc cggcagaaat gtgctgtttg acattccgaa tgccgcacag
      961 ctgataacaa acgggataag ggatctgcag tatgtatgct catacacagg tgaagtttca
     1021 aactgtcatg atgtgattac ccagttcaaa gccgattata atatctcacc gtcaatagat
     1081 atctccacgg cagtcaagac tcacgaatat acaaagctga agcttgataa gatataccgt
     1141 gcgttcggca tcgaaaacag gataaaggaa aacactacca ccgatgaaaa ggtgctgaag
     1201 cccatgataa aggaagcgct tgagcgcttt agcggttctc tgctctatct tgacgaacag
     1261 cagcacatcg ttgcactcgg cggtgcagag cttgaagaga gcctttacag cgaacagttc
     1321 cgggcatata aggagcggtg caccacagat gacatttcat caggtattcg tgccgcacaa
     1381 aagatgtttg aaaatttcat taccgacaac aaaaatgcag agttgcgttt atgcgtaaat
     1441 gagcttcccg acacggtaaa gatgcctgta ttgcttaatt cgtggcggtt tctcaccgac
     1501 aactttatga tacaggaaac tttcagacaa tttatgaaaa aatacaacga gggagtgatg
     1561 taaggtggag gcttttcgta acgaagaaat cgcgggactt ttaggcgact tttatcaggc
     1621 gcatgccaac gaaaacagcg aggtaatgga acagtcgctg atgaagatgc agaacctgct
     1681 ttacacaggt gaagccgaca gcactccgtt caaagactat ttcaactcgg tattcggagg
     1741 acgtactccg tacaacgctt ctctgtcggt aagcgactat ccgaaagagt cgataatcag
     1801 agagctttta caaaacacat tcggctgtta ctattcagac ccggatatca aggtactgtt
     1861 caactttgag caggacggcg aagtaaggct tacatataac gaagacggtt tcacgcttga
     1921 acagatactc tactatctga gtatcggcag aagtgacggc gataagtccc gtgagggtcg
     1981 tttcggcgta ggttcaaaga gcgtatttat gaatgtcgaa tggctgaaac tgcgttcaaa
     2041 taacttcaat ttcaggatcg taaacgatgc cggcattctt aagataagag agcttaatct
     2101 gactgctcct gtattcaaag gtacagagat catcttcaag gtatcggaag aagaacaggc
     2161 aagaataaaa gaaaaccttg ttaatcttac tatccacaag ggcgaatata taaacctcgt
     2221 tgagttctgc tttgcattta tcagaaaaaa acaactcgga agattcggtg aggctgaaaa
     2281 ccccaaccgt accttcaata tagcagtaat ggatatgggc aagccccttg ccgtatataa
     2341 ggttatccgt taccagaaga cccccgataa tgacccgacc gtgcgtttcc ttcagaacgg
     2401 caagagcgtt atcgagttca tctggcacga aaacgagggc tttgtatacc tcattccgtt
     2461 tgccgtgtca gccgccagac gtgatacgct tgtcaaggtg ctccttgata agtacaacta
     2521 cttctctacc tatgagctga ccggtcttat caaatccaca ggcgaggatt ttataaagga
     2581 aaagctgtcg gcattcttca taagcgtacc taactcatat atcaccactc acagaacagg
     2641 catacgccat gaatgtgagg aagaggtttc cgaagcgata aacagagatc tcaaatttat
     2701 gatagacaag tactcccgct actttgttct tgaactttta caaaggacaa attccgagaa
     2761 gtactatatg cgccccaagc actatgtgtt tgaatttttc agcaactatc tgacgaccag
     2821 tgtaatggta ggcgatctgc gcagtgtatt ccacaacagc atttcgctac tgctccccgg
     2881 caagactgaa gctatgccct actccgaggt aaagcagctt ggtttcttct ctgtgaccga
     2941 tcacgtttca aagcaggaac acgaaagcgg agctgcattc accgaatata ttgaggacga
     3001 acttgttaaa atgcgcaaag atgttgcgga tatgcccgaa aacatcataa tggcttgcta
     3061 taactggata gatgaagaaa ccgaggaaga gggcagagaa ttttgctaca acttcaactt
     3121 tgacggcaat acctatgttc tcgacagcga gaaaaacccc gctatcaagg actttgacct
     3181 aagcttcggc ttcagaagca tagtaagcct gaagctcggc aagtacgtta taaacgatgc
     3241 tgttgccgat gaagaagcgc ttatcggcgc acttggactg tttgacacaa tgtacggtca
     3301 ggactacaag atcggaatga gatacttcca gttcaacgtt cactaccgtg acatcaccca
     3361 caactttgac gttgcacgaa cgacggtaca taacctcaaa cgtgcttatg actgcatagt
     3421 ttcacacaag aacagctttg aaaatcatca gatatataac gaagtcataa atatgctgat
     3481 aaacagcttc acgcagggta aagatacaat gactttcctg cgcgaaatga aacagcaagg
     3541 cagtgacatc acactccagc ttgata
//
LOCUS       DAWAOG010000182         3522 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3400_length_3522_cov_2.314104, whole genome shotgun sequence.
ACCESSION   DAWAOG010000182 DAWAOG010000000
VERSION     DAWAOG010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3400_length_3522_cov_2.314104"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    441..450
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          999 a          656 c          942 g          915 t
ORIGIN      
        1 ggatataagt gccgtcatca agcttgtagt agcctgtatc ggtgatagcc gtaacattca
       61 cggcatcgcc gtaatgcttg aatgaaacga cttttgaacc ctgcaagcct tcttttcttg
      121 ccgagcaact tgcggttttt atatacatag taccgctgcg cttttcttct gtccatgcag
      181 gtttgggagc ggcagtagtc gcgggggttg tagcggctga agtcgatgtc ggagtagtat
      241 ctgaagcggt cgtggtcgct tttggagtcg tagcagcttt ggtcgcggtc acttttggag
      301 ttgtaactgc tttggtcgtg gtcgcggtcg tttttttggg ggtatcggct gtttgtgaag
      361 atgtaacaag tcggcttgtt tcagacggaa tgctttctgc gagtgccggt gtggttgata
      421 tgggtgatga aacagtgccg nnnnnnnnnn gtcatagctt atcgcatttt cgtttgcctg
      481 tacaccaaac catgagaaac gacttgacag aacacaaaga gcaactacag ctataaatcc
      541 gcctgcggca caagaaattt taaaagcatt gccgcaaagc tttttctttt tggaggggta
      601 ggaattttcc gctttcagtt cttctgctgt ctctttgttc gtgctcatgg tcatcacctg
      661 cctttatgag attataaaca tattttcact gttacatttt aacacgaatg tgtatggtgt
      721 gtcaactgat acagtaaaac cggataaaat tttagttgga atgcacaaat aaacgtgcaa
      781 tgtttaggct aaatcacaat aaactaagaa acatacttag gttttatcaa aagtgtacga
      841 ttttttatgt cgcgatagtc ggaaaattgt acaaaaggtc gaaaaggtta caaaacggta
      901 acaaaatggt tacaatttgt tgcaggtttg taaccacttg tgatatactt agtactgacg
      961 gaaaagaaat gctgtctttc atgacggaaa gaaaagcaac cttaaaggta tggggcgggc
     1021 ttgagcccgt tccgactttt aacgggatat gcgttaatct atcacctgtc gttcacagcg
     1081 gcaggctatt atattattgt gttgtgtacc gggcggtatt gtattacagc ccgtatttat
     1141 ttgtataaac ggaaaaggaa agagtatggg aaaccttaaa atacccgatt accgcaacga
     1201 gatcgaggaa accgtaattc ccgacaatga cagtaaaaat acagaacccg atcagaatac
     1261 ggttcagact gagagtgata gcggagcggg tcggacggaa ggaaacaacg aagaggaaga
     1321 aagcagaaag tcttttgttg ctgtagcaat ggataagatc tttgagtttt ttgttgcggc
     1381 ggggcaatat ttttgccata ttatcgctga ctttttttca aaacccgctg tgtatgttgt
     1441 acacggtgca aagtttgtat ggaaacacac ggctttattc agagatgcgg tcgaaagaac
     1501 gttcaaggag atcggaaact ttttcttctc tccgctgatc aagtcaaagc gtgtgcttca
     1561 gcgcactaat aaacagatca aggcggcaaa ggaacaaggc gataacaaga aagcggcaag
     1621 gctgtacgga agtatagcga agaatgcggt gtttggcaag cagggacttg ctgtaacgct
     1681 tttcaactac gccgcaccga tcatagcgat cgtgtttctg atgagtgttg tcggatatgc
     1741 taccggaacg aattatgcga taaagcttac ggtcgacggc aagtttgtcg gttatgttga
     1801 gagtgagcag gtattcaatg atgcagagcg cataatgcag gaacgtataa cctatatcgg
     1861 caacgagaaa aagataacta tggagccgag ctattcgctt gaaatgctcg gagatcagga
     1921 atatcttaca aaatatcagc ttgcaaacaa gctgcttgaa atgagtgata ctcctataga
     1981 gtacgcatac ggtatgtaca taggcggcaa gttctacggt gcgctgatcg acaatacggc
     2041 agtcgaagcg gagcttaaga agatactcga cggttatcgt acaaatgcaa aggacgaaga
     2101 cgttgcgttt gaaaaagata tcgaatatgt acccgggctg tatctgtcgg acagtatcgt
     2161 ttcaagtgac gagatcataa agctcgtaaa ctcgaaaaag gaagaggccg catattatac
     2221 ggtcgttgaa ggcgacagcc actcggggat ctgttctaag cttggcataa gcatggatga
     2281 gcttgaagcg ctgaacccgg gcataatgga cgaggactat gttctcagag ccggtgaaaa
     2341 gctgctcaag acgcaggaag ttcctttcct gtcggtaagt atctcacgaa ttgaaaccta
     2401 tactgttgat gttccttatg ataccgagta tcaggacgac gatacacgtt atgaaggcgt
     2461 tgaaacagta tcgcaggaag gcgaaaaggg caaaaacaag ataactgcca aggtttacta
     2521 tgtaaatgat gaggaagtca agaggactat acaaaaaacc gaaagagtga aagagcctgt
     2581 gaccgagatc atattgcagg gcacacttcc tccgcagtca tcgcattatt cggacgttct
     2641 gggaatgggg ctggtgggac ggcggttatg ccggacaccg tggtgtcgat ataggtgcgc
     2701 catacggaac ggacgtatat gcaggtgcaa gcggtacagt aatctttgcg ggctgggaca
     2761 acggcggtct gggaaatgcg gtcatgatcc tccaccccga tgggtatacg actgtatatg
     2821 cgcataacag tgaaatattt gtaagtgcgg gacaggaggt aaatcagggt gaatgtatag
     2881 ccgcagtagg cgaaaccgga cttgcttacg gctgtcattg ccactttgag gtaagatacg
     2941 gctctgaaag acttaacccg aggtattatc tgagcggatt gccggatccg gggtattgat
     3001 tttcaattga ataggtgata tgaatgcaac cgctgtgtct gagggcacgg cggttgtttt
     3061 tttattgcaa tatggttata aacactttct ttctttcata aatttactgt agaacacccg
     3121 aaaggaaagg acgtgcagta tgaacgagct gatgacaaga gaagagtttt tgagtgaacg
     3181 caatgcgata tatgagcctg acgctgttgc cgatgaaaaa acggacgagc cgtcaaaagg
     3241 tccgaggata gttattcccg gagcggggaa agatgagcaa ctgacagctg acggcattac
     3301 ggtatcggaa atagtgataa aaaacagaca gagcaggcag tatgcgggac tgatattgtt
     3361 tataacggca gtggcgggag cagttgccgg agctgtgctt tacggtgata tgacggtggt
     3421 catatcggac gaaatgctga aaaattatct ttcagggagg atacacggcg gatttttcgg
     3481 caacgcggca gcttcattta tcgcggcgct gatatggtgc at
//
LOCUS       DAWAOG010000183         3517 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3407_length_3517_cov_2.262854, whole genome shotgun sequence.
ACCESSION   DAWAOG010000183 DAWAOG010000000
VERSION     DAWAOG010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3407_length_3517_cov_2.262854"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1095 a          686 c          897 g          839 t
ORIGIN      
        1 ggtgatgaca gataagaata tgataacggt atcattttat cagatattgc attcggcggt
       61 ttttcataca atacctgtta tgattatgcg ggcgggtgta ttataacgga tctgcacccg
      121 gtacagtatg acaggtatta caattgttcg atacgcggca gtatccgagg agagagaaat
      181 taagttacgc caccgtactt taaagtattg tgggtgcaga aggggatagt tatgtagttc
      241 ccccggttac gccataaacc ggactgcatc gctgtgtgcc gtttttcgtc acggcaggta
      301 ttcaggaagg aatggtaaaa tggtagaatt aaagaacgtc gacgttcatt tcaaggacgg
      361 cggcggagtt gacgctttaa acggcgtcaa tttgcgtata gaggacggag agtttgtctt
      421 tatagtcggt gacagcggcg caggcaaatc gacattgctg aagctcctta caagagagat
      481 agcaccgacc tcgggcagag tattcgtaaa cggatacaat cttgccaaga taaaaaacaa
      541 gaagatcccg tatttcaggc gttctatagg aatgatattc caggactttc gcctgatacc
      601 cgatctcaac gtttatgaca atgtagcgtt tgtactgcgt gttacgaaca aaccaaaaaa
      661 gacaataaga cacagagtgc cgtatgttct gaaccttgtc aagcttgtcg agagagcaaa
      721 agcgtatccc gacacgctgt cgggcggcga aaagcaaaga gttgccattg caagagcatt
      781 cgcaagcgac ccgaagctca taatcgcaga cgagcctaca gcaaacattg acccggcact
      841 gtcgtatgac atagtaaagc tgctcaagga gatcaacaag tgcggtacta ccgtaataat
      901 ggttactcat gagcacagcc ttgtcgaata tttcggcgga aggatcgtca gtcttaaaaa
      961 cggcgaagtc gttttcgacg aatatgtaga aggtgaggcg caggatgaat aactttactt
     1021 atcttataaa gcaaggtata aggtcggtat ggaagaaccg ctttatgtcc tttgccagcc
     1081 tttgcattat gacagtcagc cttattttgg ttgcaatgtc ggctatagtt atgcttgact
     1141 gcggaataat tctcgacaat gtatccgaca agaacgagat aagcgtttat ctggacgatg
     1201 acgcagatgt aaagcatatc ggtgaagtgc tcaaaagcaa taccctgacg gaaagtgccc
     1261 agtacatttc tcccgaagag ggcctgaaaa agatgataga gcagtacagc gagcaaaagg
     1321 agctgtttga aagtctgccg tacaatcccg ttccagctac ctatatggta actataaacg
     1381 acatcgacag gatgagcacg gcggtacagc agttcaaagc gatagacggc gtttacaagg
     1441 taaatgctcc tatggatttt gcaagcttta tcaaggatct gagagcaacg tttactataa
     1501 tcggaatagt gcttatagcg gcgctcggca cagtaagcgt tatcataata tcgaacacga
     1561 cacgactcag cgtattcgca agacgtaagg aaatttcgat aatgaggata gtcggtgcta
     1621 caaattcgtt tataaaaaca ccgttctttg tcgaaggaat gttcattgga cttttgtccg
     1681 gtgttctgtc gtggtttgtg acaaagctcg tttacgaaaa tctgttcaat cttttcacgc
     1741 agaacctcgg aatgtggaat gcgctcggaa tgggcgacat attgcagttc aacgatataa
     1801 cctggtatgt tcttgccgct tgctgcggtg cgggcgcttt acttggggct atcggtacgg
     1861 tactcagtac aggcaaatac cttaaggtat aacggcgaaa ggaataaaga tgaagctgaa
     1921 aaaaattctg cgcactctga cttcattcgt cacaattttt gcgatgacat caggtataat
     1981 cgtaacgtat gctcccgcag gcgcagagga tcttacggtc gatgatatca aagacagaat
     2041 agagcagata aaacaggaca acgaacaaag acgtgatgag ataagccgta tagagggcga
     2101 tatctcggat accaaggaaa accttgaaaa aacagcaaag cttctagatg agcagaagga
     2161 gcttgtagac tactattaca atctggttta ttataaaaat caggatataa ccgagcttca
     2221 gagcaatata gatactctga cagatgagat agcgaagaaa gatgaagcta tcgcaactgc
     2281 cgagaagaac atagaaaagc ttgacaagca gaacaaggat aaccttgaaa agtttgcgca
     2341 gatagtcaga actatgtaca catcgggttc aagcgatatg ttcggtgtgc tcgcaggctc
     2401 tgtagatttc tatgacctta tgataaacag cgaggtcgtg ggaaagataa gtgagcagaa
     2461 cctggagttt atgaataagc ttacagctga tatgaagcag ctgtcttccg ataaaaaggt
     2521 acttgaaaca gacaagaagg atcttgagga gtcaaagaaa cagcttgacg gtaaaatgac
     2581 agagcttctc agcgaaaaag aggaattgaa cggtctggta tctgatgcgg aaagcgccaa
     2641 gagcacatac gagtcggact atagcaatta ttcggctgct gtaagcaacc ttgaaaacag
     2701 acagagcagt cttgaatatg agataagtgt cagcgaggct gatatcgaag catatgaaga
     2761 gcagataaaa gagcttataa agcagcagac aaatcccgat aaggcatatc aggagggcga
     2821 gtggtactgg cctgttccgg gacgctcgta catatcctgt aacttcggtt gggacgatga
     2881 tttcggcaga tggcacaaag gcgtggatat cggtgacggc ggaatttacg gtgacagtgt
     2941 gcttgcatct aaggcaggaa cggtcatcgt ggcggaaaca agctacatac cgggctacag
     3001 ctacggtatg tatgttgtag tagatcacgg cggaggatat accactacat atgcacactt
     3061 aagtgcggta tatgtttctg tcggacagga agtggcgcag ggcgaggctc tcggtgcggt
     3121 aggcagtaca ggctactcga caggtcctca tctgcatttt gaaatcagac tgaacggtga
     3181 acccgataat ccgttcaatt atgtaacaat ggtgtgataa agcgcaccac acaggaacaa
     3241 ggaaagggtg atccccgatg aataaaaaag tatcgcttgg cgttacaata agccttatag
     3301 cggtagcgtg tgccataacc ttcgtactaa cgatgactgt gtcgctcaat atgtacaatt
     3361 caatggtagc cggcatacag gagcgggaaa ccataaacgc aaagataaaa gaagtagata
     3421 cgtttgtccg cagctcatcg atatataagc ttgatgagaa tgagcttatc acaggtatcg
     3481 caaacggtta tataagcggt acaagcgaca aatacgc
//
LOCUS       DAWAOG010000184         3509 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3422_length_3509_cov_2.048639, whole genome shotgun sequence.
ACCESSION   DAWAOG010000184 DAWAOG010000000
VERSION     DAWAOG010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3422_length_3509_cov_2.048639"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3168..3177
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          894 a          821 c          761 g         1023 t
ORIGIN      
        1 ggatatatat acccatactg ccccgctttc ggcatcgctc tcatagcccc agacccttac
       61 gagaatatct tcagtggaaa gatagatgcc cttgttgagc ataagaagct ccatcagctt
      121 gtattctatc ttgggcagga caattgactg caacggtcct ttaagctcgc agctgctgag
      181 attgagcgac acattgccga atgtcataat atcgggcgta aacctgtctt ttctgcggag
      241 cattgctctg atacgtgaaa gaagttcgcc cattacaaac ggcttcggca ggtaatcgtc
      301 cgcacccata tcaagccccg ctatcctgtc ctcgacttca gcctttgcgg taagcaggag
      361 aataggagtg gtgtttccct cgctccttag tgttttaagc acctctaagc cgctcttttt
      421 cggcatcatt atatcaagaa ttatcccgtc atattgttca gctcttgcgt aatcaagagc
      481 gtcggcaccg tcataaaccg tgtctaccgt ataattatga taggtcagaa tgtcggtcac
      541 agcctcagcc atactgacct catcttctgc aaacaggagc ttcataatat caccgtcctt
      601 tttcgatatt gatattatag taacgttagc ttagaacagc cttagagcgt atgagatttt
      661 gtgcaaaatc taaagctcag catttcttat atccggcgaa cgttgccgga acaatcagac
      721 atatacaaaa aaagctgccg cagtgcgaca gcttttaaaa gctttaatta ttcggcagat
      781 gtttctgtgc ttgtttcaga tgaagactcg gctactgagg aatcagcaga agaagcgtca
      841 gatgaagaag aagaatctgt cgatgaagct gacgaatcgt cacttgtaac ggaagatgtt
      901 acagatgatg ttgcggagct tgttgtcgaa gaattgtctg tcttattgaa gtttccgctg
      961 tacataacga tgaagttaac aacgagagcc acaacaaaga agatgattgc ggcaaccttt
     1021 gtaagtctgt tcagcttagc ctcgtttgaa cgtcctgaat ttctctgata atagttgtct
     1081 gcactaccgc ctgtaatcgt ctgcgacata ccctgctttg tatcctgcat aagtaccacg
     1141 agtacgatga aaacacaggc aacaaccaga acaatagcac tgattatttc taatattccc
     1201 atttatatgt cctccttata ggaatacggg tatgtccgag tgtctgttta tcgtacaaac
     1261 ccaaaattat tctaacacgc aaatgctatt ataacataaa aagaaatgaa atgcaagtgt
     1321 tttttgggaa ttattttaat gcagaaagcg cttttttgcc gatatcatga cgataatgtg
     1381 ccttttcaaa gcttatagtg tctactgcct tataggctgt gtcgagagcc tcctgtaatg
     1441 tatcgccgta cgcggtaatt ccgagaactc ttccgcctgc cgtaaggaac tttccgtcct
     1501 ccagctttgt gcctgcgtgg taaacgaatg cgccctcgca ctgaccgttt tcgtcaagac
     1561 cgtttattac cttgcctgtt tcgtactttt cgggatagcc gccgcttgcc ataacaacgc
     1621 aggcgcatga cttgtcgctc cactttatat caagcgtatc gagcttttct tcccagacag
     1681 cttcgattat atccataata tctgtttcaa gtaaggggag aaccacctgt gtttcggggt
     1741 cgccgaagcg gcagttgtac tcaattacct ttgcaccgtt cggtgtcagc ataagtccga
     1801 agtacagaca gcccttgaat ggtctgccct cagccgccat agcctttatt gtgggcagga
     1861 atatcttttc catgcattcc tgctgtttat cgggggtgta tagggggttt ggagctatag
     1921 tacccattcc gcccgtgtta agaccctcat cgttatcata agcacgcttg tgatccatag
     1981 agcttatcat aggctttact gttttgccgt ctgtgaacgc aagaacggtt acctcaggac
     2041 ctgtcataaa ttcttcgatt actatctcac ttccggactt gccgaatttt ccgtcaagga
     2101 gcattgaacg tactgcgttt tctgcctcct gttcgtcctt ggcaattatt acgcccttgc
     2161 cgagcgcaag accgtcacat ttgataacgg cagggaagct gttctgcttt ttgatgtaag
     2221 ctatagcctc atctgcgtct gtgaatgttt cgtaagctgc tgtagggata ttgtacttct
     2281 tcatcagagc ctttgagaat accttactgc cctcgagaat ggcggcattt gctctgggcc
     2341 cgaaggtctt aaagccctct gcgttgagct tgtccaccat acccataacc agcgggtcat
     2401 cgggtgctac gaatacatag tcgggtctga gcttctttgc aagctctaca cagccgtcga
     2461 tatcggttgc ctttacatta aagcactcgg catattttga gataccgccg ttgccgggtg
     2521 cggcatacag cttgtctacc ttgtttgatt ttgcgagagc ggttattatg gcgtgctctc
     2581 ttccgcctcc gccaattaca agtacgttca tcttgattaa aacctttctt ttttagatct
     2641 tgtctgtttg actattcaac aataagcccg agcagttttg caaacattgc cgcttgccat
     2701 acatcgacct tagcgccttt aagttctgtt cctgtatccg atacggtaag accgtcgata
     2761 ttacaggagg taaagtcaag tcctgcaagc tttgtcccga agaacaccgc ttttgcaagc
     2821 gtgacgtttt taagctccgt ctgttttatc tcgcacgaag aaaacagagc agaggagaag
     2881 tcggtgtccg ttatacgcac cgattgaagc tttgaacgct gaaagtccga cagctcaaag
     2941 ctgccgccgt caaagctgac atttttatga tatgagccct tgaaagccgc tccgacagcc
     3001 ttgcatgagg taaaggagca ggctctgaaa tagctctcgg tgaaatcaca gtttgatata
     3061 tcacagcctt tgaaaacaca gtcaatgaat gtggattttg aaaaatcaca ggctgtaaag
     3121 cgactgccct tgaacagaca gttcttgaaa tacagggaag agaagtcnnn nnnnnnngct
     3181 ctgcatcaat atccgatatc ggtatcagct ctgaaggcag tttcaccgat ccggaaaatg
     3241 agtttgatct ttccgtcatg tgttgacaga gtagtcaaaa gtcattgaat tgagcgtgta
     3301 ttcaagcgac tttgtgacct gctcttcatt atcatcaaga tgaagagcta ttacagtata
     3361 gactttattg gcgtgacgag caaaatatat gtgctgtatc gcagaaacag aatattctcc
     3421 gtccgatgat ttcacagttc cgtaaaacac atcggtactg ccgattttga tcatgctttt
     3481 tgttgatgaa acaatattat cgccgacag
//
LOCUS       DAWAOG010000185         3509 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3421_length_3509_cov_2.245802, whole genome shotgun sequence.
ACCESSION   DAWAOG010000185 DAWAOG010000000
VERSION     DAWAOG010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3421_length_3509_cov_2.245802"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    418..427
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2771..2780
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          985 a          724 c          912 g          868 t
ORIGIN      
        1 cgattatgct ctgcacgaag ggatcaactt tctttttgac ataagggagt ctcaggcgtc
       61 atacgacggc ccgtggttta cgggcgtgga tgaattgaag gctgtatggg tgaaataaga
      121 tacagtgagg tggcgacgtg aacggtatta agctgaaaat atcaaaacca aaggataagc
      181 ttcccgcagg ttcaagcaag ctgttcggca atcctgatgt gtgggacggc ttcgaatggc
      241 cgtgtatcga ggaaaacggc gaaaagtacg acttgacctt tatgtgtcag attgactgcg
      301 ctaaggcaga aggctttgac gaaagcggac tgcttccgaa aacgggaatc ctttatttct
      361 tttatgacct tgatacaatg ccggaaatgt cgaacagccg gtcggcacgg gttctttnnn
      421 nnnnnnnata tgacgcaaag ctgtgatttt ataaaagatt atctgccgct tttgcgtatt
      481 ggctcgtttg aaacggagga tgcggaaatt tgcttcaacg gtaacggttc gctatgcttt
      541 tatatcgaaa aagagaaact tgtagcgtgc gatttttccg aagtgaaagc ggcgcagata
      601 taattcagag aaggatcacc aaaagaaacg ggcacggaaa atatacaagt tataaacaga
      661 tggaatccga ttgcgaattg ttttaaggag aaaaacctat gagcatatgt gacgaattaa
      721 aaaaacaggc aatgcagaca tacaccgatg gaatcgacaa atccatcgaa acagcggcgg
      781 cgtgctttcg gctttgcgcc gaggggttat cccgattttc cgatgacggc ggaggaaact
      841 acccgataca ggatatttca ctgcttattg cctgctcggc ggtatcgggc ggttggaaca
      901 tagcgggctc tgccgacaag gagcgtatgt ctgaaaggct gaagctgtat gtgacaaatc
      961 tcaacagcct gtgtgatgac ctgccgcttg cactgaccgt tcccggatat gcgaatatcg
     1021 ggcggaatct catgttgccg ccgcagataa cggcgtttac cgaacatctg acgcgcctgt
     1081 tccaaacgct tgacgagcat gaaaaaatgg cggtcgtcac aatgcactat ttgatgctgt
     1141 atgcggtgtt tgaagaatgg ccgcaggtgg atgtgctgaa aaacgcctat gaaaacgcct
     1201 tagttcagcc ggaggaagcc tacgagtcgc ccgagagcaa tacggcaggc gttccgttct
     1261 cgcatacata cgccgccccc gcacaaccat ctgcctctac ccttgcccga tatgagcgca
     1321 acgcaaagcg ccgctggcgg ccgctgaaaa tttaccttat tgtaacggga ctgtttattg
     1381 cgttggtgct tgggttatat ttttacgccg cacctccgtt ttctgaaggt caggagattg
     1441 tggtaaagga tatcaccgga cagtcggtga aaatgcttcc ggagtccgag cctgtggagc
     1501 ttgccttggt ggggctgacg gattataacg gcgtcgtccg cctgttttac ggaatgataa
     1561 caggcattgt ggtgtaccga atagtgtcaa tcatcgccgt gtttatcata aatttacttt
     1621 atcgaaggag aaatgcaaaa aaagctgctg ctttaagagc aggcaattat ataccgtaag
     1681 tccgattttt aacaatatgt tcggcattga ggcgtgcttc cttatgacga tgctgctcgg
     1741 cggaatactt tgtgttaaga ttgcctttgg taaaaagctt ggactaactg cacagttctg
     1801 ccttacaaag gacggcacgg cgccgctgcg ctgtctgatg atggcggcac tctgggcagt
     1861 gcttgtattc agcatagcgc ccgccgcaat ctttattgtg tggcttgccg ctgttgccgt
     1921 tatactgctt gttgccggaa catttagcgc tatgactccc ggttacagct atgaagagct
     1981 tatatctatg ggctattccg actacgaggc aagaaagctg attcaaatag caggcggcaa
     2041 gataaacgga atttaaatca atatatcata acattaaccg aaaaggagta ttttaaatga
     2101 atgatattga attattgaac gaagggctta aaatgattga agtcgatacc atgaaagcgt
     2161 ttgaatattt ctcaaaatct gcggagcttg gcaacgccga ggctatgtat tatctcggct
     2221 taatatatga atcgggcgac ggcgctgaaa tgaatgtgaa aaaagcgctt gaaatgtttg
     2281 aaaaatcaag agcggcgggt tgtaaatcgg cggcaagcaa tatcgggaat ttatatcact
     2341 acggacacgt tgacggcttg gagccagaca gcgaccgtgc gtttccatat cttttaagag
     2401 cggcgcacga tgatataatc aatgcaatgc gtccgctttc tgaaatctat ctgttcggca
     2461 cagacgcaat tttcccaaat taccgtcttg cggcatattg ggcattaagg ggaattgaag
     2521 aggatgatac cgattgctgg tatttgattg ctgtgatgta tgagcatggt ttgttttttg
     2581 agaaaaacct gtcatatgca aagcaatgcc tcaaaaatgc attgccgatg gaagtggcaa
     2641 ataaatatct tcatgataaa atgtatacgt tcgtaattcc gagaaagccc gatttagcaa
     2701 gctttgatga cacggcggcg gatttttttg cggacgaact gccgcaagga cttgacgaac
     2761 aggcaaaagc nnnnnnnnnn ctataggagg ctttaaaaat gataagtgat aaacttgaac
     2821 gaatcgaaga gctcgaaaaa gagctttcct atcttgtaaa cgattcaatg acgttagagg
     2881 agaagctgaa aagcctctcc ggcgcgtatt gggaggcgag tcattccggc tacggcgacg
     2941 ctatggcaaa caagctgatg ggcggcgaag aggatgacca aacccgcctt tgggagaaaa
     3001 actgtgagaa taaatacaaa atcaaagcac tcattaactt gctcagtgag ctaaagaaag
     3061 agggtgatag cggatgttga cctgtcttta ttgcggctca tcccgtttac aaaccacagg
     3121 aggagacgtc ggcaatccct ttgaccgcag acaagaaata acgatctgcc ttgactgcgg
     3181 acgaacggta ggaacagagc cggatttgtc ggagctgttt caaagcgcga tctgcatcga
     3241 aatgacggct gagggcggcg gacttgttca aaaggcaaag gcggaatttg cctttcagga
     3301 cggcgaatac gatttaagcc gtctcgaata cccgctttgc cgatataagc gcttaaaaat
     3361 cagcaaagat acagcggacc tcggcggcga gatttttgta agcattaccg atgtgccgta
     3421 tgaaataaca agacaatata ccgcttacgc tcccgataat cgaccggtgg cggaaacgct
     3481 gaaaatcaaa gtttatagcg atatacaat
//
LOCUS       DAWAOG010000186         3507 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3426_length_3507_cov_1.747683, whole genome shotgun sequence.
ACCESSION   DAWAOG010000186 DAWAOG010000000
VERSION     DAWAOG010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3426_length_3507_cov_1.747683"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1804..1813
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          884 a          848 c          741 g         1024 t
ORIGIN      
        1 gcagtgaatg cacttgttat tgtcacggaa catatgcttt gagctgtagt cgatatcgta
       61 ctcgttcttt tcgccctcaa aatatgtagt gtcatcaaca ccgagctcct tacacagttc
      121 ctgaagctcg cagtttccgt ttctggagca cgcaaggcag tccatgttgt gatccgaaag
      181 caggagctgt aatgtcatct ttctgctctg taatacagca ggggagtgtg tcataatgtt
      241 cttgccctcg tcaaactttg ataagggata tacgcatgat gcaacaaggc ttcttgcacc
      301 tgtcatttca actacgcata tacggcacgc gccgatagcg ttgatatcct ttaaccagca
      361 gagagtagga attttgatgc cgttttgacg acaggcttca aggatagttg taccttcttc
      421 gaccgagtac tctatgccgt taatctttat attaaccata gtaaaattta ttcctttcag
      481 tgatccgtca gacgggatca ttccttaata atagcaccga acttacactt agccatacaa
      541 gcaccgcact tgatacactt ggttgtgtca atggtgtgag gctgcttgac tgtacctgag
      601 attgcaccga cagggcatcc tcttgcacag gctgtacaac ccttacattt aaggtcgatg
      661 atcttgtatg tgagcagatc ctgacatacg cctgccggac acttcttatc ctgaacgtgt
      721 gcaatatact cgtccttgaa gtagcgcaga gttgataaaa cggggttggg agctgtctga
      781 ccaagaccgc acagagagtt gttcttgata tagtaacaca gcttttccat cttgtcgaga
      841 tcttccattg tcgcgttgcc cgaggttatc ttctcaagca tttcgtaaag acgctttgtg
      901 ccgatacggc aaggtgtaca tttaccgcag gattcatcaa cggtgaactg aaggaagaac
      961 ttcgcaatat ctaccataca gttatcctcg tccataacga taagtcctcc tgaacccatc
     1021 atcgagccga tagctataag gttgtcatag tcgatcggga tatcaaggtg ttcggcaggt
     1081 atacatcctc ctgaaggacc gcctgtctgt gccgccttga atgccttgcc gttggggata
     1141 ccgccgccga tctcgtaaat aacctcacgc agtgttgtac ccataggaac ttcaacaaga
     1201 cctgtgttgt gtatcttgcc gccgagtgca aatacctttg tgccctttga cttttccgta
     1261 cccatagatg caaaccattc cgcaccgtta aggattatct gaggaatgtt agcgtatgtt
     1321 tcaacgttgt tgagtacggt aggcttcttg aaaagaccgc tttctgccgg gaagggagga
     1381 cgaggtctgg gctcgcctct gttaccctcg atagaagtca taagcgctgt ttcctcgccg
     1441 catacgaatg cgcctgcgcc aagtcttata tcgaggtcaa agtcaaatcc gctgtcaaaa
     1501 atgttcttgc cgagaagacc gtattctctt gcctggttga tagctatctg taaacgctgt
     1561 acagcgatgg gatattccgc tcttatgtag atatagccct gtgtagcgcc gatcgcataa
     1621 ccggcaattg ccatagcctc aattacaacg tggggatcgc cttcaagaac agaacgatcc
     1681 atgaatgcac cggggtcgcc ctcgtcggcg ttacagcata catacttctg atcggcatcc
     1741 ttgacaaggt ttcttgcaag ctgccacttc ttgcccgtag ggaatccgcc gccgcctctg
     1801 ccgnnnnnnn nnnttaagtg attttatttc attttcaccg attgtttcat catatacaag
     1861 acgtgttacc acacgacctt taagaagatg ctcggaaacg atttctgcaa tgtcatcttc
     1921 ctttaccatc gagtagaatg tgccctcggg ataaactatc atgataggac cgagcgcgca
     1981 cagaccgaaa cagcctgttc gaactaccat tacttcatcg gtaagaccgt tcttatcgat
     2041 ttcctcctgc agtcttttag cgatcttatc gctacctgaa gaagtacatc cggtaccacc
     2101 gcatatcaat acttgtgaac gatacatata ttagtttcct ttcagatttt acatagcgga
     2161 gtttatcgtg tacttcgtta caacgttgcc gccgataatg tgctgttcga taacttcaac
     2221 agccttttca ggtgtcatct tgacataagt aaccttttcc ttaccgggag cggtcacttc
     2281 aactattggt tcaaattggc aaagaccgat gcagccttcc tgtattacca ttacatcatt
     2341 tatgcctttt tcctgtattc cgtcggaaaa tgctttaaga acaggtcttg cgcctgccgc
     2401 aattccgcag gttgccatgc cgacaacgat ctttgtgcag tctgtcgctt catttctgag
     2461 attgacaaca ttctgcatct tgtcgcggat agcagctaat tcttcaagag atttcatatc
     2521 ttaaaattat cctttcgtgg tgttagtatt tcttgccgcc gcttacttcg gctgtattat
     2581 cataaagata acgggtcaaa aataccgtca cttccgataa atttatcggt acattgccga
     2641 gaatttttct taactgcctc gtatcaagag tgaagcttct ttcgtcagtc gaatagttgt
     2701 agataaaatc aatatggcag tttgcattta taagagatat gagcgtttcc ggcatatctc
     2761 cgagcggcat tctgtctatg cttgacagca caaaaacagc ctttacccga gtgcctttac
     2821 ctttttcaga gcttatatcg aagctaccgc cggtcatttc agatgcagtt ttgaaaaaag
     2881 gtacacccag ccctgtattt ctcgtttttc cggtagtata aaacggatca acgactttcg
     2941 acaattcttc ggcactcatg cctgagccgt catcttctat aacaacggta agcgtatcgc
     3001 cttttgtgtc gatattaacg gttatttcta ttaaagaagc ccgtgcgctg attgaatttt
     3061 gcactacatc aagaatgtta agagaaattt cagaaaacat tattctgtat attttgcaag
     3121 aataccgtcg atctgatcaa cgttaagctt accgtaaaca tcatcattta ccgtcattac
     3181 gggagcaagt ccgcaagcgc ctatgcaacg gcaggcatca agtgagaaaa taccgtcctg
     3241 tgtgcatttt ccatccgata ttccgagctt ttcggagagc tttgcgaata tatcgcccga
     3301 gccctttaca tagcaggcag taccaagaca gactgagatc ttgtacttac ccttaggata
     3361 aagcgagaac tgtgagtaga acgtggaaac accgtatacc ttttcaagcg gtatacccat
     3421 ttcttcggct attatcgact gaacctctat aggcagatag ccgtagattt cctgagcctg
     3481 ctgaagcacc ggcataagcg cgccttg
//
LOCUS       DAWAOG010000187         3491 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3446_length_3491_cov_2.277066, whole genome shotgun sequence.
ACCESSION   DAWAOG010000187 DAWAOG010000000
VERSION     DAWAOG010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3446_length_3491_cov_2.277066"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1286..1295
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1091 a          730 c          877 g          783 t
ORIGIN      
        1 cggagaagac ggaaaagagt ttgtgaaatt ggataaggat caatttgaag acatatccgc
       61 tccgaatgat catattgtcg aagattgttt ctatacatca gaacaacgaa tgcgtttgat
      121 tgttataaag cctgtgctga aaggtaacag caggtggact tttaaaacaa gtgaaaacaa
      181 gacaatagag gcaagaattg aagatgaacc gtggaaagat aatttcgatg aaggtaatgt
      241 taaattattt gccggaattg gcttagaagc gttagttgct ttacaataca aaaaagatga
      301 atacggaaag cctattgatg atacaacaaa gtatagcata aagaaagttt attcaacttt
      361 tggtgaggat aataaacaag aaacgcttcc cgaataatta acatataacc gctccctaac
      421 cggagcggtc aatttttata cccaaaagaa aggacggtgt accgccaatg accgaaaggc
      481 agaagaaatt cgcagaatac tacgctcagt gcggtaacgc cgcccagagt gcaatacagg
      541 caggatacag cgagaaatac gcaggtcaga acgctgacaa attactaaaa aatactaaca
      601 tagcagacta tatccgtgaa ttaaccgaag ccgcccagac tgcccgaata atgacggcaa
      661 gagaacggca ggcgatactt tccgatatgg caaaagatgc gcagaacgag ctgtcggacc
      721 gtatcagagc tatagacacg ctgaataaga tgacggggga gtacacgcag aaggtcagta
      781 ttgacggaga tgtgggagtg aagatagttg acgactgtta agctcagcga cattatagcg
      841 ccctcgttct acgatctgca caaggatata aaggcagaca gacacacgca ttactggctc
      901 aaaggcggca gaggctcgac aaaatcatct tttgcatcaa cagagatact gctcggtatg
      961 atgaaagatc ctatggcgaa tgcggtcgtt atccgaaaag tcggactgta tctgaaagac
     1021 agcgtttatg aacagctcct gtgggcgata gaaaggctcg gcgtgtcgca tttatggcag
     1081 tgcaggcagt cgccgcttga gcttgtatac acgccgacag gacagcgtat tttatttcgt
     1141 ggcgcagaca agccgaaaaa gctgaaatct accaaagtca gaaagggcta tatccgctat
     1201 gtgtggtatg aggaggcaga cgagttcggc ggtatggaag agatacgcac catcaatcaa
     1261 tccctgctca gaggcggtgc gacatnnnnn nnnnngttca atccgccgaa aagccaaaga
     1321 aactggataa acagcgaggt gcttgttccc cgctcggaca agatagtgca tcacagcgac
     1381 tatcgttctg tgccggcaga atggctcgga gaacagtttc tgattgaagc aaagcacctt
     1441 gagcagacaa agccagaaca gtacaggcat gaatatctcg gagaggtaac cggcacgggt
     1501 gcggaggtat ttacgaacat tactatccgt cctatcacgg acgaggaaat aaagtcattc
     1561 gatcatatca agcgtggtat agactggggc tacggcgcag acccgtttgt atatataaca
     1621 gctcatttcg acagcaagcg aaacaggctg tttatttttt acgaattttt caggtgcgcc
     1681 gcaaagtatg acgttattgc aaatgcaatc cgcaaggaga atacacaaaa tggtacaatc
     1741 attgccgagt ctgccgagcc ccgctcaaac gatgagcttc gggacagagg ttctcgcata
     1801 aggacggcgg tcaaaggtcc gggaagcgtc gagcacggta taacgtggct tcagaacctt
     1861 gaagagatta ttattgacgg tacacgttgt ccgaatgccg cccgtgagtt caacgaatat
     1921 gaacttgatc gtgacagcag gggagaactg aaagcggact tccccgacaa gaacaatcac
     1981 accatagacg ctatccgtta tgcccttgag gactatatcg gcaggaagat agtgaaatca
     2041 acgctcagta agcggaaatt aggcatttat taaggagatt ttatatgata acatcaccga
     2101 ttttcacaac ggacaaaacg gcggagatga taacgccgaa agtagcacgg gattatatag
     2161 aaaagcacga taagtacgaa atgccacgtc ttacgatgct ggataattac tactgcggca
     2221 gacagcacat ctgcgacaga cgtaaaagtg acgatatgct gtgcaacaac cgtgttatga
     2281 taaaccacgc cgcatatatc gcaaagttta catcttcgta tctgatagct actcctgttt
     2341 cttacagcgg taaagatgat acggatatta cggcaataac cgactgcctt tcttatgccg
     2401 acagcagtac gcaggacgca gatcttgcgc tcgatgccgc aatattcggc agagcctacg
     2461 aacttatcta tatggacgct gacagccgcc cgaagttcgc ccgtatcact ccgctgtccg
     2521 catttgtcgt ttatgatgat acggtggagc aaaatcccgt atttgcggtt tattattatc
     2581 ccgtttttga gccgggcaaa agtacgcctg agtgcttcaa gtgtcagctt atgaccgata
     2641 tgataacgca ggattttgag cttacaagca acttcggaat taagtccgag ggcgagataa
     2701 tatcgcacta tttcggcaaa gtaccgctga atgagatcta taatgacggt cagcgacagg
     2761 gcgattttga gcaggtcata agccttattg acgcatataa cacgttgcag tcagacaggg
     2821 ttaatgacaa ggagcagttc gttgacagcc tgatgtacat caaaggtcag atactcggtg
     2881 aaacagacga tgaaaaggct gaaacctaca gcgatattca gcgcaacagg gtcgtagagt
     2941 tgtcgcagga cggtgaaata ggctttctga cacggcagtt tgatgaagca agtgtggaag
     3001 tgctgagaaa gagtattgtt accgatattc acaagatttc gggcgtaccc gatatgtcgg
     3061 acgaaagttt tgcagggaat gcttcgggtg ttgctatgaa gtacaagcta ttgaaccttg
     3121 agcagattac caagacgaaa gagcgatatt tcacagaagg cttacgttac cgccttgaat
     3181 gcttttccaa cataatcggc ataaagggcg gtcatatcga tccgaagctg atagacataa
     3241 cctttacacg ctcacttcct caaaatgagc ttgaattatc ccaggtggtg gcaacgcttg
     3301 acggtaaagt gccgcaggaa actttactgt cgctcctgcc gtttgttaaa gatcctcaga
     3361 gtgccgcaga agaactccga cagcagaagc aggacgctat agtgacacag cagcagatgt
     3421 ttatgaacac accacttgca aggggcgaaa gcaatgaaga atccgagtaa gaaatattgg
     3481 gaggacagag c
//
LOCUS       DAWAOG010000188         3490 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3448_length_3490_cov_2.601164, whole genome shotgun sequence.
ACCESSION   DAWAOG010000188 DAWAOG010000000
VERSION     DAWAOG010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3448_length_3490_cov_2.601164"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    576..585
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          902 a          783 c          592 g         1203 t
ORIGIN      
        1 gctcatatat gtattcggac ggtctgcata ttttgtgaga atgcggattt acaagtgtcc
       61 acatggtatc gacatcacaa agaatgtttt cgtcaatatc cttggcaaca aattttcgag
      121 taaactgcga acctttagta cctccctccc aggttgtcat atacagcttg ttattatagt
      181 atggaatttt gttaaaatga attgccgcgc gggtaagtaa aaatacttga ccgtcctgcc
      241 acatcttttc acgggttata tttctgcttc ttaaatggtt tccagcaagc tccgcacacc
      301 acgcgagaag aacctgcggt tttatgtcat attttttatc gcagtcggca aactccggta
      361 tatattcaac ggtaaatttt ctgttgtcca tattaacctt tcataaatat tgaataagcc
      421 acagataccg aagtacctgt ggctcagtgt tttacttatt atccttagga actattttat
      481 cgataccacc catatacttt ctgagaactt cgggtatctt tacactacca tcggcacaaa
      541 gattattttc aaggaatgca ataagcattc tggggnnnnn nnnnnattct ggggggcgca
      601 acaacagtgt tgttaagtgt atgagcaaag tagttgccct tttccttgct cttcactctt
      661 attttcaaac ggcgagcctg agcatcgcca aggttagaac agctgcctac ttcaaagtac
      721 ttcttctgtc tgggtgacca tgcttcaacg tcataagact ttaccttaag gtcggcaaga
      781 tctcctgaac agcattcaat tgttcttaca ggtatatcca ttgatctgaa tagatctact
      841 gtgttctgcc acagcttgtc aaaccacata gcactgtcct caggcttgca gacaacaatc
      901 atttcctgct tctcaaactg gtgaattcta taaacgcctc tttcttctat gccgtgagca
      961 cccttttcct tgcggaagca cggagaatag cttgtcaacg tgtaagggag cttttcttct
     1021 tcatttatag tgtctatgaa tttacctatc atggagtgtt cgcttgtgcc gataagatac
     1081 aggtcttcgc cctctatctt atacatcata gcgtccattt cggcaaatga cattacgccg
     1141 gttactacat tactgcggat catataggga gggatgcaat aagtaaatcc tctgtctatc
     1201 ataaaatcac gcgcataggc taaaacggct gaatgaagac gtgcaatatc tcccatcaga
     1261 tagtagaatc cattacctgc aactcttctt gcactgtcaa gatctatacc gttgaatctt
     1321 tccattatct cggaatggta aggtacttca aagtcgggta ctacaggctc accatatctc
     1381 tttatttcaa cgttttcact gtcatcctta cctatcggaa cagaaggatc aataatgtta
     1441 ggaattatca tcattctctt cttaacttct tcaccaagct cttcttcttt cttctcaagc
     1501 tcttcaagtt catcagagaa ctgctttacc tgtgtcttga cctgttctgc ctcatctttt
     1561 ttgccctgag ccatcaaacc gccaatctgt tttgacagct tgtttctgtc tccgcgcaga
     1621 ttatcagcct tctgctgagc ggctcttaat tctttatcaa gctcgataac ctcgttaacg
     1681 agtactattt tttcatcctg aaactttttt ttgatatttt cctttacagc ctcgggattt
     1741 tctcttaaaa acttaatgtc tatcatttaa tatgcctttc tttttaaatt atttgttatt
     1801 tgcgagccga tttacaatat cggtcaaatc ttcatcagaa tagaaatcta tttgtatagt
     1861 actctttttt ttgcccggta ctatattaac atttgtttca agcgcctttg caagactgat
     1921 ctctgcctcc acgtagaatg tttccttttt cttttccggc ttttcgctct tatcaagtgc
     1981 ttttaccatt ctttccattt cacggacgga aacatcctta ccggcagcct tatgagcaag
     2041 ctctaccata tcctttgttt ctttaacgcc aagcagaacc ttgcagtgac ctgttgacaa
     2101 ttcattattt gacaccattt cagctattat aggcggtaaa ttaagcaaac gcaatgaatt
     2161 tgcgattgaa gatctggctt ttccaactac tcttgcaaca tcttcctgag tcatgccgaa
     2221 cttttccata agctctttat agccgttagc ctcttcaatc ggatttagat tttcacgctg
     2281 taggttttct ataagcgcta tctgctgtgc ctgctgatcg gataattcct ttataagcac
     2341 cggtacttcg gtaagaccgg ccatcttagc cgctcgccat cttctctcac cggccactat
     2401 ctgatatgta ccatcaggca ttgatcgaat aagcaacggc tgtattattc cattttgtct
     2461 aattgaatca gctagtgttt ttaaagcctc aatatcaaag ttttttcttg gctgtgattt
     2521 attaggctca atatctgtca gtttgagggt gcttatacct tcttcatctt cgtcaaagct
     2581 gttatcatca aaaatactgt taaagttctg gtttaaaact cctcgttttg ccatacaccc
     2641 tccttaatta ttgagttttt ttattatttc tccggaaagc ttcatatagc tttccgcacc
     2701 ttttgagctc ttatcataat atataaccgg cagaccgtga cttggggcct ctgaaatttt
     2761 tacatttcgc ggtattacgg tattgaatat tgatgacgga ggaaagtttc tttttatatc
     2821 tctcattatt tcattagact gcaaaagtct cttatcaagc atagtaaaca ctatacccat
     2881 aagtccaaga cttttatttg aggtcttctt gactttcttt acagtagaca agagttgcgc
     2941 caatccttca aggctataat gttcacactg cataggcaca ataaatttgt cacttgcaat
     3001 aagtgcattt attgccagta tgccgagtga cggcagacaa tctataacaa ttatatcata
     3061 atcgtcttta agctgtatta gtgccttttt cagttgatga tttctttgtt caatctgaag
     3121 caaatgcatc tctgcctcag ccagttgagc atttgcaggt ataagactta cgtttttgca
     3181 attagtagcg attactgctt cctgaggtct tacattagac attacaacat catatgatgt
     3241 gatcttcaga tttttctttg acacgccgta tccggaggta gcattgccct gaggatcaaa
     3301 gtctaccaat aggacttttt taccctttgc accgagagcg gcactgatat tgacagcaga
     3361 cgtagtttta cccacaccgc ctttttgatt tacaatagaa attattactc ccatagtacc
     3421 tccttttacg tcactttata attatatcac actatgggag aaaaataaag tctttttgca
     3481 aaaaaacgtt
//
LOCUS       DAWAOG010000189         3482 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3464_length_3482_cov_2.304640, whole genome shotgun sequence.
ACCESSION   DAWAOG010000189 DAWAOG010000000
VERSION     DAWAOG010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3464_length_3482_cov_2.304640"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1043 a          792 c          884 g          763 t
ORIGIN      
        1 cggtatgccc ataggtatgt cgcttgtcgg cagaaagtgg gacgaggcta cgctgataca
       61 gacggcagac gcttttgaaa agacgtttga cagacgttac agcgaggttt gagaagggac
      121 ggtaacaata tgaaattata tcctaccata ggacttgaaa tacacgcaga gcttctgaca
      181 aactcaaagg tattctgcac ctgctcggct gagttcggcg gcgagcctaa ctccagatgc
      241 tgtcctgtct gctccggact tcccggaacg ctccccgtgc ttaactcaaa ggctgttgag
      301 tacattataa aagcaggcta catcatgaac tgtgagatat cacgctttac aaagtgggac
      361 agaaagaatt atttctaccc cgacttgccc aaagcatatc agatatcgca gatgccccgc
      421 cccgtgtgtc tgggcggtca cgttgacgta acggttgacg gcgagcagaa gacgatgaga
      481 ataaaccgta tacatcttga agaggacgca ggtaagcttg tacacgacga cattgagcgt
      541 gtgagccttg cagactataa ccgttgcggt atcccactta tcgagatagt taccgagcct
      601 gatttccatt cagccgccga ggtcatagcg ttctttgaaa agataagact tatgctccag
      661 tatgcaggcg tatgcgacgg taagcttgaa cagggttcaa tgagatgcga cgttaatatt
      721 tcgcttgccg aaaaggattc agacaagctc ggaacaagaa ccgaggtcaa gaaccttaac
      781 tccacaaagg ctatacaaag agccattgaa tacgagatat accgccagac agagcttatt
      841 gagaacggtg aaagagtagt acaggaaacg ctccacttca atgacgctac gggtgaaaca
      901 agctcgcttc gttccaagga ggacgctcac gactaccgtt atttccccga ccccgatata
      961 ccgccgataa tctttacgga agaagagctt gcggctataa aggcggacat tcccgaaatg
     1021 ccggagcaga gagtagcgcg ctacacaggc gagtacggca tttctgcggc tgacgcaaag
     1081 gtgctgacag attcaaagcg tgtaagtgac ctgtttgaaa atgccgtaaa ggcatataat
     1141 aaccccaagc ctataggcac gttcatagtg gttgagctta tgagaagaat aaatctcggt
     1201 gagcttgatc ttgataatat gaaatttacc gcagaggatc tcgcaaagct tatagagctt
     1261 gcggaaacag gcaaaatatc aaaggataac agaaagataa tcctgcgtga aatgcttgaa
     1321 acaggcaaga agcccgaaac tatcgccgaa gaacagcagc tgtggataaa ggaagacaac
     1381 gcactgcttg aaaagacgat aaacggcata atggaggcta atcctaaggc tgtcgagcag
     1441 tacagaagcg gtgaaacaaa ggtattcggc ttccttatgg gacagtgcaa caaggcactc
     1501 aagggagcgg cttcacccgc aagtataaag gcaatgcttg aagaaaagct caagggctga
     1561 tttataaaaa aagaaaggca agaaagaaat gtttttagag aacaagtacc gcactcacac
     1621 ctgcgaaatg ctgagtgaaa acgacatcgg caagaccgtc cgtgttgcag gctgggtaga
     1681 gaacatccgt gaccacggag gaatcaagtt tatagatctg cgtgaccatt acggttatgt
     1741 gcagatcaca ttccagaaga acgagggtct tcttgagggc gtgaacaagg agtgtgccgt
     1801 tacaataggc ggtacggtaa taaagagagc agagggtact tataacgata agatatccac
     1861 aggaactatc gagatagtag ccgaaagtct tgaggtactg ggcaaggtaa ctgttgaaca
     1921 gctcccgttt gaagttccca cctcgacaga aacaaaggaa gatatacgct taaagtaccg
     1981 ttatctcgat ctgcgtaata agagaatgca caacaatatc gtactgcgtt cacaggtcat
     2041 ctcgttctta cgcagtaaga tgaccgagat gggatttctt gaaatacaga cccctatcct
     2101 ttcaacaagc tcacccgagg gagcaagaga ttaccttatc cccagccgta agcatcacgg
     2161 aaagttctac gctctgccgc aggcaccgca gatcttcaag cagttactta tggtatcggg
     2221 ctttgacaag tacttccaga tagctccgtg cttccgtgat gaggacgcaa gagcggaccg
     2281 ttcacccggt gagttctatc agcttgactt tgaaatggca tttgcaacgc aggaagacgt
     2341 ttttgcaaac gcagaagagg ttttatccgc tgtatttgaa aagttcggcg gcaagcccgt
     2401 atctcccgca ccattcaagc gtatccccta cgccgagtca atgctgaaat acggtacaga
     2461 caagcccgac cttcgtaacc cccttatcat aacagagctg tcagacttat tcgttgacag
     2521 cgacttcaag cctttcgcaa acaagtgtgt aagaggtatc agagttcccg gtatggctag
     2581 tcagtcaaag ggcttctttg agaagatgga ggcattcgct aaggagatcg gcatgaaggg
     2641 tcttggatat gtcaaggtag acgagagctt caagtatctc ggtcctatcg acaagttcct
     2701 gaatgacgag cagagagaag agcttaagaa cagatgcgct cttgaagttg gcgatgttct
     2761 ttacttcatt gccgataaca agaagatggc gcctaagttt gcaggtcaga tccgtacaga
     2821 agtcgcaaag cgtatggata tgatcgacta tgacagatac gaaatgtgct ttatcgttga
     2881 cttcccgatg tatgaggagg acgaggaaac aggcaagact atattcacgc acaacccctt
     2941 ctcaatgccg cagggcggtc ttgaggcgct gatgacaaaa aatccgcttg acatactcgc
     3001 atatcagtat gatatcgtat gcaacggtgt tgagctttca agcggcgctg taagaaacca
     3061 cgaccttgag ataatgataa aggcattcga gatagcaggc tacacagagc aggacgttgc
     3121 cgaaaagttc ggcgcgctct acaatgcgtt ccagtacggc gctccgcccc acgcaggtat
     3181 ggcacccggt gttgaccgta tgataatgct cctcacaggc gatgagagca taagagaggt
     3241 tatcgcattc ccgatgaact caaacgctca ggatcttctg ctcggcgcac ccacagaggt
     3301 aaccgaacag cagcttcgtg aggtacacat aaaggtacgc cagaaaccca ccgtctaagc
     3361 ggcgagcggc gatgcgccgc accaaattcc gcctttttga attgtcagag ctatccgcag
     3421 gatagcgaaa cggcgggaat aaacggtatc ataaatttta taaacgaatt acggcagata
     3481 tg
//
LOCUS       DAWAOG010000190         3474 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3477_length_3474_cov_1.730331, whole genome shotgun sequence.
ACCESSION   DAWAOG010000190 DAWAOG010000000
VERSION     DAWAOG010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3477_length_3474_cov_1.730331"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1197..1206
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2502..2511
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1027 a          694 c          842 g          891 t
ORIGIN      
        1 acgtaatgct gatcattgag ttccagcacg taaagcccgg taaaggtgcc gctttcgtaa
       61 gaaccaagat cagaaacgta attacgggtt cggtagttga aaagtcctac aaccccacag
      121 ctaagttccc cacagcatac gttgagagaa aggatatgga gtacacctat aacgacggtg
      181 acctttatca cttcatggat tccgaaactt acgaagatgt accgataaac aaggcagagc
      241 tttcagacaa cttcaagttt gttaaggaaa atatggtatg taaaattctc tcttacaagg
      301 gtaaggtatt cggcgtagag ccccccaact ttgtagagct tgaaattaca gatacagacc
      361 ccggtttcag aggcgataca gctacaaacg taactaagcc cgctactctt gaaacaggcg
      421 cagagatccg tgttcctctg ttcgtagaga tcggcgatat tatccgtatc gatacaagaa
      481 caggtgaata tatggagcgt gttacaaaca agtaatcgca gaacgtgatt actatatgta
      541 actaccgaaa gtaaaaggag gatttttact atgacagttg cagaaaaggt atcatacatc
      601 aagggacttg cagaaggtct gggtgttgat gattcaacaa aggaaggcaa agtgctcgct
      661 gctatcgttg acgtgcttga cgatattgca ctgacactcg gcgatataga cgaagagctg
      721 aacgatgtgg cagacgttat gaccgatatg gaagaaagcg ttgactgcct tgaagatgtt
      781 gtttacggca atgatgagga cgattacgat gactgtgatt gtgatgactg ctgtgacgag
      841 gacgacttcg acgatgatat gtacgaagtg acttgcccca gctgtggcaa cactatcact
      901 gttgacttcg atgttatcca gtcaggcgaa attccctgcc ccaactgtgg tgaaacactt
      961 gagtttgacc tcagctgctt agaggacgag gaatgcgatt gcggttgcca ccacgaccac
     1021 gaataattga tgtttacagt aaccgccgag cgtaaagcac ggcggttatt tttgtatctc
     1081 tacattgaac taatcaactc tttttagtca cctgccttga ttttttatag caatagtggt
     1141 atacttacaa cataaacggc ttttgccgat cgaaaggaaa cgaaatgatg agcgaannnn
     1201 nnnnnnaatg aaattcttgt aaaaaacgga aaagagccga ttgactggca gatagaaaat
     1261 atataatttt ttggaggtta tcattatgag cgaatgtact cacgactgca gttcttgctc
     1321 gcaaaactgc ggcgaaagaa cgacccctca gagctttctt gaaaagccgc atgagctgtc
     1381 gcatataaag aaagtaatag gaatagtaag cggtaagggc ggtgtcggta aatctatggt
     1441 aacttctctg cttgctgtga ctatgcagag aaaaggcttc aagaccgctg tgcttgatgc
     1501 cgatataaca ggtccgtcta ttccaaaggc tttcggactt cacgaaaagg ctaccggaga
     1561 taatacaggc atataccctg ttatgacaaa aacaggcatt gaagtaatgt cggtaaatct
     1621 gcttttaccc gatgaaacag accctgttgt atggagaggt cctgttatcg caaatacagt
     1681 aaagcagttc tggaccgatg taatatggaa cgatgttgac tatatgtttg tcgatatgcc
     1741 gcccggaacg ggagatgttc cgctgacagt attccagtca ctacccgttg acggaattat
     1801 cgttgtaact tctcctcagg agcttgtttc gatgatcgtc ggaaaggctg tcaagatggc
     1861 tgagatgatg gacgtaccgg ttctcggcat agttgagaat atgtcttact acgagtgccc
     1921 cgactgcaag tcgcgtcaca gtattttcgg tgacagccac attgaagagg ttgccgcaaa
     1981 gtacgatata aagaatatcg caagaatgcc tatcaacccg aagctggcgg cggcttgcga
     2041 taaggttctt attgagcttt acgatggcga ctggctcaac gaaacagccg atatgcttga
     2101 aaagctgtaa gcgacttata atataatgac cgcagacacg ctctgcggtt ttgaatattt
     2161 gtattgtcaa ccgtatataa agccaggttg acagtgaaag gtaattcttt ttatgaaatt
     2221 aaaagagcag gtactgctta tactcatgag cagtaaaggc ggatatgtat cgggcgaaga
     2281 tatctcaaaa cagttgtacg tcagccgtaa tgctgtgtgg aaagctataa actcgctcag
     2341 agcagacgga tttgtaattg acgcaataca gaacaaggga tatcttttgt ccggcggaga
     2401 agattacgac tttacacaat acggacttat gcgaataaga caattgcttg gaaaatctgc
     2461 cgccgatgcc gaaataacca taaaagatac cgtgacctcg annnnnnnnn nctgcgaata
     2521 aaacggtgct aatagcgaac gagcagacag agggcagagg cagacgaggc aaaagtttct
     2581 actcgcctgc tgacagcggc atctatatgt cggtgctcct ccgacccgac ttcggcgcgg
     2641 acaaatcgtt cttcataacc gcaggtgcgg ctgtgtctgc tgtaagggcg gtaaagaaag
     2701 tctgcggtat cgatgccggc ataaagtggg tcaacgatat tatgcttgac ggaaaaaaga
     2761 tctgcggaat acttaccgaa gctgtaactg attttgaaag cggaagtttg caatatgccg
     2821 taatgggact gggtataaat ataagccgcc cgaaagacgg ctttccgtca gaaatatccg
     2881 acatcgcgtc aagcctttat tccgatgagg ttgccgacaa cgagctaaaa tgctcgcttg
     2941 ccgctgaaat actgaacaac ttttttgata tatacggaca tacatcggtc gagcggctga
     3001 tcgacgaata caaaagatat tctgttgtga taggcaagcg tatcaaagtg atttgcacgg
     3061 gctcggaata ttttgctacc gctgttgaca ttgataacga tgcacggctt gttgtaaaag
     3121 atgacaaggg taatatacat acgttatcct ctgccgaggt ttcggtaaga tagcgtcata
     3181 agaggtataa atgaataaac tgcttaatca tctgcgtatt cgcagtgaac gactttacat
     3241 aatattgaaa acgtttttca agtggcttgt gctttccggg ataacaggac tgtgttgcgg
     3301 agttataggc tcgctgttcc atctgtgtat ggaatttgcc actgaatttc gttcagggca
     3361 ttcctttatg gtgtacttcc tgccgatagc gggacttgct atcgtatttc tgtacagcat
     3421 atgcggactg aaaaacgacc cgggtacaaa tataataatc acatcaataa ggac
//
LOCUS       DAWAOG010000191         3470 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3485_length_3470_cov_2.010835, whole genome shotgun sequence.
ACCESSION   DAWAOG010000191 DAWAOG010000000
VERSION     DAWAOG010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3485_length_3470_cov_2.010835"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    644..672
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2994..3003
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1019 a          659 c          875 g          878 t
ORIGIN      
        1 atcctgccgt ccgcatttat aggaacattc gtatgctgtt atcagtgtca gctttcttta
       61 tcgtgttcaa ataacatatt ggcgacatta agaggcagac gtgaaacagc tccgcattat
      121 tataagatat atattgaacg ttttgcggtg tgctgtatag gttgctttct gcttggtata
      181 gcggatgcgg ggatatcttt tttgctcgga ccgatattcg ttatatgaac cccttgaaat
      241 ttttacttca tgggtgtata atgttatcag taaaaattgc ctttggcatg aaagaaggat
      301 aacattatgg aaaaaatgga agtattactg gaaacctccg cacgtcatat ccacgttaca
      361 caggaggctc tcgaagcatt gttcggtaag ggtcatgagc ttacaaagaa gaaggatctt
      421 tctcagcccg gtcagttcgc ttgcgaagaa agagttacta taacaggtcc gaagagatcg
      481 cttgccaatg tatctatatt aggccctgta cgtcctgcta cacaggttga gctttccgct
      541 acagacgcac gttctatcgg atgtcccata tttgtaagag agtcgggcga tatcgcaggc
      601 acacccggct gcaagatcac aggtcctgag ggcgagatcg agannnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nncgtcatat ccaccttgtt cccgaaaccg ctgaaaagtg gggcgttaaa
      721 aacaagcacg ttgtaagcgt taaggtgaac tcggacggca gaagcgctgt actcggtgac
      781 gttgttatca gagtaagcga gaatttcgca gatgctatgc atattgatac cgatgagtca
      841 aacgctgtta tggctgctcc cggtatgatg gttgaggtta tcaagtaaat cccggtcgat
      901 aattgtaata tgaacaaatt gatctctgcg gcttttcggt cacagagatt tttttgtaaa
      961 catattgaaa ttgtccgaaa aatattgtat aatattattg ctacataatg gtaatctgat
     1021 tgaaaataag ggggaaatat gaaaaatcct ttcaaaagaa ttattgcggc gataacagcc
     1081 gttacgcttg tggcaggaag tgtgccgctt aatacgatag catcggcaca gctggctcct
     1141 gtggaaactg ccggtcacgg tatgctttca tcggtgtcgg aagaatatat cggatatatg
     1201 cattccggcg agggcgacaa agcggggaat acaccgtcta tgcttgatgt gtcgtacctc
     1261 ggcgaaagct acgcacagct tacgcggacg gcagatgaaa aattcccgtc atcgtatgac
     1321 ctgagggatc acggcagggt gtcgccggtt gagaatcagt caccgtatgg tacctgctgg
     1381 gggtttgcat cgcttggttc aatagagtca cagcttataa cgcagtttac cgatatttct
     1441 ttttcaaaaa aacaccttgt ctggaacgca tatgagaacg attatatgcg ggaattcttc
     1501 aacggggtaa gaagcaaaga ggatttcgat aatccctatg atatgggtgg ttttgacagc
     1561 cttgcaacgg ctacgctggc ggagtggaac ggtcctgttt attcctacag attgccgtat
     1621 gacaaggacg ataccgatga gcatctgacg ggtgaaagcg attttcatct tacagatgtg
     1681 tactttgcga cggcacaaac ctatattacg gatgggtacg ataatatttt agaaaaagat
     1741 atttccgaaa aaatcataaa agagcttgta agcgaatacg gtgcatcgac gatatcgtat
     1801 tgcagtgatg atgcttacta caacaatgaa tattacactt ccttttgtta tgacgagaat
     1861 aaagtttccg accatgcggt ttcggttgtc ggatgggatg atgatttttc aagagaaaaa
     1921 ttcggcacaa atgatgacac ccgtcctgaa aaagacggcg catggcttat aaagaacagc
     1981 tggggtgcca actggggaga tgacggatat ttctggctgt cgtatgaaga cccgactata
     2041 ggctgggtgg caaaatatat cgttgatagc aaggacaact acagcaatta ttacggtgtc
     2101 ggaaagatgt ggtcgctgaa cgcttcggcg gatacgttct ataaagacga aaatgccaag
     2161 aaatacggct atatgtcgaa tatattcacg gcaaagggag atgaacagct tgaagcggtt
     2221 tcgttctata cgactgatgt aaatacgcag tatgagataa ctgtttatac cgataccgac
     2281 aaggaaaacc ccgtaagcgg cagcatagct ttaagcggtc aggcgggaac ggaaaaatat
     2341 cccggttatc atactgtgga gcttgacaag gcggtcgcac tgacaagcgg agagaatttt
     2401 tccgtggtgg tcaagcttac aaatccccga tatggatatc cgttgccggt cgagatatat
     2461 tataatggct tttacaattc ttcaccggag tattccgatg acgggagagt atcaatgtac
     2521 agtgaaaacg gcaaggattg gaatgacgtg tcggagctgt cctatgtaac cggcggatat
     2581 acattatgcg ctaccggctt ttgtcttagt gcgtttacaa atcctctgcc ggagagcaaa
     2641 accgcatcga aaaatgtgcg tttttcgata atcgaaggtc ctgttgcgct tggaagcaag
     2701 cttgagctga gcggagcgga cgagatatgg tacagcatag acggcggagc ggctatacat
     2761 tatacaaagc cgatcgttat tgaaaaggag tgcactgttt cggcatggag caagacggac
     2821 ggcagaagcg gcaatatcgt cagccgtaca tatacaaagg caaagtcggc gcttacggaa
     2881 tatgcggtca aatacggaga taagaagctc tttgtgacac cggatatgtt cggagaggat
     2941 accgaagtga ccttgcctgc agctgtgagc ggcgtttcga tacgccccag aggnnnnnnn
     3001 nnngaggcag tgacgttatt atgatcgacg gaaaaacagc cgtatccgat gaatggtcgg
     3061 aggaaataga gctgaccgca ggcgaaagcc gtgatatagt tattacttca tctgctgacg
     3121 gcaaggataa aacagaatat acaatacacg tttcgaagca gaatatcgga tatgatacgg
     3181 acaaggaaac gataaagtat gacgaaagca gatatatagt aaaggacaag gacggaaata
     3241 ttcttgcaaa cggcgactct ttatcggact ttacggagca gaaaataagc gtttataaca
     3301 aagataatga gcttattgcg actgaaacga cacctaagag atatacgata atacccgatg
     3361 taaaagcttt ggtttacaat gaagagaaaa cgctttacac attcagcgat aaatatattt
     3421 gttcatataa tcccgatatg agcgacgcgg tagcattgga cgaggaaaca
//
LOCUS       DAWAOG010000192         3470 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3484_length_3470_cov_2.069693, whole genome shotgun sequence.
ACCESSION   DAWAOG010000192 DAWAOG010000000
VERSION     DAWAOG010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3484_length_3470_cov_2.069693"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          860 a          863 c          684 g         1063 t
ORIGIN      
        1 tcatcagtca gtaccatctt aacataaaca taactcggga acagcttgtt ctcataagtt
       61 ttcgtctttc cgtcgtgttc ctcaaccact gtttcggtag gaaccataac ttcttcgatt
      121 aaatggtgca ggttattgtt ttcaactacc ttcataatgt tgccggcaac cttattttcg
      181 taaccggaat aggtatggag aatataccat tttgcctcag acattcttta gtccctccta
      241 cttgaaaata cttagcctgc tgaactcatg ttgagcgcta atcttaacag agtagcgaac
      301 agagtgtcaa ggccccaaac acagataccc gatactacca gggtaaccag tacaacaacc
      361 gtgttgttga atactgtctt cttgcttggc cacacaacct ttttgaactc ggattttaaa
      421 tccttgaagt actttacgat ggatttcttg ggctttttgg aaagagcggc cttcttgctg
      481 ttcttttcgt tagccttttt cgctttcgct gcttctttag tcaagcttgc atctttagcc
      541 atcataaacc tccttactta gtttccttgt gtggagtatg cttcttgcag aacctgcagt
      601 acttgttcat ttcaattctg tcaggatcgt tcttcttgtt cttcttggtg ttgtagttgc
      661 gctgtttgca ctcagtacac gcaagggtaa ttttaactct cactttcggc acctcctatt
      721 tttttgccct tctgggcttt agcctccctc tttgcctcag actttttctt cggcagggtt
      781 tattgtgaat taaaaaattg cacaaaaaaa taaaccccta atgaggtact ataatcataa
      841 cacattatga taggtttgtc aataggagtt tattaaattt ttgtgttttt tgcggggctg
      901 acagcgcaat ttcacttcaa cttgaaataa agcaccacct gaaatgcggc gagtgccgca
      961 aacagtacaa gaactatcac ctgtgcaatg ccccacgtct gcattctgaa tataacaagc
     1021 agacagcctg cggcaagaat tgcggcattt ataagaaaac gcttgtttcc gccctgccct
     1081 gctttcttaa acggattttt tacctgctta ttcatacttt tgactgtata cgcttagcca
     1141 taagcgtatt cttcatcagc attgctatcg tcatagggcc tactccgccg ggaacaggtg
     1201 ttatgtatga gcatttcggt gcgacttcat cgaacttgac atcgccgcag agcttattgt
     1261 ctgcgtttct gttcataccg acatcaataa ccactgcgcc gtcctttacc atatcggctg
     1321 taacaaagtc cgctctgcct actgcgctga ctaatatatc tgctctacgg caaacctctg
     1381 cgagattctt tgttctcgaa tggcatacgg ttactgttgc gttctcgtgg agtaaaagca
     1441 tagccatagg cttacctact atattcgatc tgccgataac aacgcactcc ttgccctcga
     1501 ccgatacgcc cgtgctgtga ataagttcca taacgcctgc gggtgtacag ggaaggaacg
     1561 agtaatttcc tatcattatc tttccgacat taacggcgtt gaatgcgtca acgtccttga
     1621 gcggtgaaat agcctcgatg accttttctt cgtcaagatg cttcggaagg ggtaactggc
     1681 agagtatgcc gtttacctta tcgtccttgt tcagcgtgtc gataagttcc ataagctcgt
     1741 cctgcgttgt atcttcgggc agtgcgtact cgtatgactt aattccgcat acctcgcagg
     1801 cctttttctt gttgttcaca taaacacggc ttgcctgatt gttgcctact attattacgg
     1861 caagacctat atcgagcttg tcacgctcta tctcggcacg gatctgctct ttaacgcttg
     1921 cagatactgc ttttccgtca ataagattca ttatttttcc tccgttttat caggggtatt
     1981 ttcttcgttg ctttcggatt tttccgtctt ttcttctttc ttttcggtct cagccgatgt
     2041 ttcctcgtta tcggaagctt cgtcatcgac tattatgctc ggtgcttctt cgcctgcctg
     2101 cttcatagcc ttcttagcct tagccttttc ttcaagcatc ttctgacggg cataatacgc
     2161 ttcgttcttt tccttgcacg cttcgctgtc cttatacatt acatagccac gtttcttcgt
     2221 cttgaaaatc ttgccgagta tgaacaatgc aagtccgact ataacacaac cgccggctac
     2281 tatctgggat accttgaaag gtccgatgaa caggctgtct gtacgcagtg cctctatcca
     2341 gaagcgacct aagccgtacc atattatata cagcaggaaa atctcgccgt caaaggtttt
     2401 cagcttcttt gagtagaaat gaagtgcgat aaagccgata aggcaccaca ggctctcgta
     2461 aaggaaacac gggtgaacaa gtgcaaacag tccgtttcct gcggcatcaa gctctgcctg
     2521 ttttgcaaca accacgggat cgtttataat agttgtgcct gtcatacccc acgggagcga
     2581 accagccgta ggagcaccgt aagcctcctg atttacaaag ttgccccatc tgcctatgca
     2641 ctgacctata agaagtccga gaccgcccac atcgaacatt gcgggaaggt ttaccttatg
     2701 gataacgcag gtaatcgccg ccgcaatcgc cgccgcaata ataccgccgt atatcgcaag
     2761 tcctccgtga cgcagatcaa agaaatttat atcggtatca atgaaaatac agtagtaggc
     2821 tcttgcgccg ataaagccga aaattatggc tacaaaagct acatcgaaaa cgtggtcggg
     2881 gataagcccg aactgcttgc acttgtgcat agcgtaaata aacgcaagaa tcacgcctac
     2941 cgcaataata actccgtacc aatagatgtc aacgccgaac aggttgaatg ctaccctgtt
     3001 tacgataata gacttatcaa acagattggg aaattcaatt ctcggcactt cgttcaccga
     3061 ttcggtcaga atgtttaata aaccgctcat gttaaaaaat cctcacttaa ttctcatcgg
     3121 caggctcgat tatcgaaatt gaattattct caatgtcgaa atcgtcaaga gccgatttta
     3181 tatcggcaaa tgtggtattt gccgtaattt ctataccatc ataaattccc acgtcattta
     3241 tttcctgggc aatcatcgca tttgcgacag attcgacatt attgaagcct gccacaagct
     3301 cgccgtaagt cttgttacgg atagtgatga actcgtcttc gtcaagacct tcttttctca
     3361 gacggaaaag ctccttttct atctctgaaa gcaccttgtc gggatctgta gattcaccgt
     3421 cagcgattac gctgaaaaat cctctgccgc agaatacgcc gacattgaac
//
LOCUS       DAWAOG010000193         3453 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3514_length_3453_cov_2.499117, whole genome shotgun sequence.
ACCESSION   DAWAOG010000193 DAWAOG010000000
VERSION     DAWAOG010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3514_length_3453_cov_2.499117"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1726..1735
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1044 a          903 c          766 g          730 t
ORIGIN      
        1 tggcataaag ctgtcggaaa aacagcttgc aaaagtcaga aagggaattg cgaagctcga
       61 acgtaacatc acccgttgga agccgccgta tcagctggca agcgaaaaca gccctaccgc
      121 cgacattgcg gattttgacg attattgcag ctcactgtgg aacggcactc aagcacactt
      181 ctataagttt aaaagcggca gatttaaaac gtatttcgtt atgagcacaa actgcgtctt
      241 tcttgcggac tacattctca gcaaggcggg aacagatata gtaaagaccg caggcataat
      301 cacccccggc gactattacg actatatgca aagtgaatat gccctccccg gcggaatagt
      361 gatcacccgc gatatttaca gtaaatataa cgttttgccg gctgaaactt aagtgaaaat
      421 ccttgaactt gacacgatag tgtgatatac tacaatagga caagaattta catttacggg
      481 taatataaag atagctgccc gcattaagaa aggacataat atgaagctca atatcagaat
      541 aaccgcagcg atcctgactt gcgccgcaat actcgcatca gcctctgcct gctcatcaca
      601 ggatacaagc tcacagaaca gcaagagcga aagcagcgct gcacagagca gtgccacaga
      661 gagcacagac agctcctcga ccgacagcac atcggaaagt gaaagctcct ctgacagcaa
      721 ggagagcacc gacagctcgg tttcttccac cgaccacgca acatcatcgg aaagcgacaa
      781 gcctgccggc gtagacggtg ttcagacaaa cggtcagctt gtcgttgata tagacggtca
      841 cacatggggc atttcgcttt acggcggcgg tgacggcgca aactatgcta cctaccttaa
      901 cgagttcaag gaaaaagtcg gctcatcggt aaacgtattc aatatggtag taccgacagc
      961 gggcgcgtat tacctccccg caggctatga aaaatacaac gcaagccacc gtgactctat
     1021 aaacagcata gcaaacaagc ttgtgaatgt tatcaatgtt gacggctatg ccgcacttga
     1081 ggcacacaca aatgagtaca tctatacccg taccgaccac cactgggaac ccctcggcgc
     1141 atactatgcg gcaaaagctt tctgtgatat cgcacagaca cccgcaaagg agctttcaac
     1201 atacaagaca gaaaccatag acggctttgt cggtacaatg tacgcgttta cagactacaa
     1261 cgagcgtatc aagaacgacc ccgacacatt cacctactat atcccctcta cagagtacac
     1321 cgcgacctac tacacgaccg acttcaaggt cgacgagcag ttcgcgcagt tccacagcat
     1381 atttgtagac cagcccgcaa gcggtgcata ttcgacattt atgggcggcg accggaagat
     1441 agtaaagata gaaaccgcca acaaaaacgg cagaaagctc tgcatattca aggacagcta
     1501 cggcaacgcg gaagttcctt tctatataga ctcatttgaa gaaatatatg tctgcgatat
     1561 ccgttacttc gacctctatg cgcccgactt catcaaggaa aacggtatta cggacgttct
     1621 gtttacaatg tgcacattct ctgcagtagg cgaaaacgca gagggaataa agaacaacct
     1681 gctttcagaa taaacgcagt gttccgaaag gaggagcgat atgcannnnn nnnnnataac
     1741 agcacttaca ctgatacttt caatgctcgc ctgcggctgc tccgatatta tccccgaaag
     1801 cggctcgaac agtaaagata catccgcata taccttagcc cccgacccgg tatcatctga
     1861 aactcccccg gacacagaaa gcaaagaccc cgagccaccc gacacctctg tccccgaaac
     1921 atcggtgacg gaaacatccg cctttgaaac atcggaagaa accgccgcgt caaaaaccga
     1981 aacgagcccc gacagctcct cagccgcagc atcttccccc gaaagctccg tccccgtaag
     2041 caacagcgat ataaacagct acagggcgat aggcgacaac ggaatattag ttgcgggaat
     2101 ggataaccac taccgcggaa ttatgttcta cggcggcaca tacggctact gcgaccaata
     2161 cgtcactgac gtgaagtcgt ttcaggcggc gctccccgag gtatcggtat attccatggt
     2221 tataccgacc tctgtcgatt tctataaccc tacagagtac aagggctaca ccgagcttca
     2281 gaaggataag atagattata tcgaaagtga gcttaagaat acaggcgtaa ccaacgtcag
     2341 cgtatacgat acgctttcaa agcacacctc cgaggagata tattcaagga ccgaccacca
     2401 ctggatgccg ctcggcgcat attatgccgc acaggtgttc tgcaagacag ccggcgtaaa
     2461 agtccccgat atcaaaaact accgcaagga aacctgcggc ggctatgtcg gctcaatgta
     2521 ttactattcg tccgatgagc accttaacaa cgaccccgaa acctatgcgg tctactattc
     2581 cccgaatgag gataagctga aaaccacata ttacgaccgt tattacagca acggctatga
     2641 cagcgatctg tttctgtgca atgacgctag ctactattac ctgtcattcc tcggcagtga
     2701 cgacctcatc gcacacataa agaccaacgc aaagacaggc agaaacttag tcgtgataaa
     2761 ggaaagctac ggcaacggac ttatcccgtt cttcaccgag agctttgata atatctatgt
     2821 gcttgacctg cgctattgcg aggtaaacgc gatcgatttc tgtaaaaagg tcgatgcgac
     2881 cgatctgctg ttcgcaaact gcgcctatac cgttgcaggc ggcaactgcg actatttctc
     2941 atatataaga aacatttaaa aagtgttttt atattcacta tttcaacgat tgtaatcccg
     3001 gcttctgtga agccggaaag ggtcgcaggg gcgtcagccc ccgtattctc cccctttccg
     3061 ggggaaaggg ggtcaggggg atagggatat aaaaagcact tcaatgagat aattgataat
     3121 atgatttaat tgcttgaaat aaggacacac ctatgaaaga acttgaatac cccttcgaca
     3181 gcggttatct gctgaaaaag aaacgctcta taaaaaaaca gctcctgtct gacggcagtg
     3241 tgcgtataaa gaaaaaaatc gccgttctcg gcggttcgac aacgaacgac atcataaata
     3301 tgctggagct gttcctgctg aattacggca ttgagccgac cttctacgag tcggaataca
     3361 accgctactg gcaggacgca gtattcggaa acgaggagct tgacagcttc tcccccgacc
     3421 ttatcttcat tcacacgtca aaccgcaaca tac
//
LOCUS       DAWAOG010000194         3433 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3541_length_3433_cov_2.737419, whole genome shotgun sequence.
ACCESSION   DAWAOG010000194 DAWAOG010000000
VERSION     DAWAOG010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3541_length_3433_cov_2.737419"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    892..901
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2385..2394
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          875 a          745 c          700 g         1093 t
ORIGIN      
        1 gagcctgtta tcagcactat cgagcctatc catagtattt tttcaaaggt tgtgaagtag
       61 ttgttaagct tttttaacat tatatcctcc gtaaattcac cgaaaaagca ccgtgagtac
      121 atctacaaag acctaacgat gtaccacggt gctttaaaag catctctacc cggtggcaga
      181 attattgtat tgttatttta gcataacggt cgggggttgt caaggagcgt cccggtttat
      241 ttatgcctgt ttaacaaacg tcgcatttcc gattttttca ggtacgctga aagttgcggt
      301 agccgggatt tcatacgttc cgctgaaatt gactttttca gcctgcaatg caacatactt
      361 gagcattttg ttctgaagtg cggtcatctg ttcatcagtg agtgagaagc tgccgtatat
      421 ttcggttctt gcggcaattt cttctgcggt tatgcccgct tcttcaagtt tatccatggt
      481 gttgttgtat atatatgcag gtgtgtatat cagatcattc ttcaaaatcg cagtattgta
      541 tgtgaataaa ttgaagcatt tttccacatc gcctgaggcg atagcttcta tttcttcatc
      601 ggtgtatatc atatctgcat attcggcatt ctcgctctca gatgaaagct cagtataata
      661 tttttgcaga tctttcaata tttctgcaac tttatcgtga tccaatgagt atttttttac
      721 cacgttagga agatttggca tttcgttgat attataaggt ctgtttttat tatcaacggg
      781 attgtcattt tctttgctcc aggagatata ctcctgaagt tcttcttcgc tacatgattc
      841 tttaacaaga ttttcaaatt ttatcgaaaa taatcccagt ttggatattt cnnnnnnnnn
      901 ngattgaata atgtcggacg agtttgatgt tgcttgtgat gtcgattggc aaccgcacac
      961 aattgcggaa acagcgagta ataagcctgt gataataaga acgtttagcg attttttcat
     1021 aaaattacct ccttaatatg agtatgtgaa attgatgttt ttgaaaaagt caagtggttg
     1081 agtgtagtcc aaagagttta aggatttatt aacatcatag tgtagatgcg aaccgtaagc
     1141 ctctccggta caactaactt ttccgatata gtcgccggct tttactttat cattttcttt
     1201 aagattaaca gaaaattctc tcacgtgcag atatctgaca acataaccat tgtcctgtat
     1261 tatttcgaca aagactcctc ttccgtaaaa tttatcgtaa aaagaatatc gcaccgtacc
     1321 gtcatagctt gcatacacag gataaccgtc aacatcaccg tttgcatcaa ttatatcaat
     1381 tccatcgtgg cgtttgccgt tatcgtaatt aggatatcgt tgcgaaatat gagttgccat
     1441 atagggtgac ttaaacatat aagaccagtt tgtcgttggt tttttatatc cgcattttgc
     1501 acagacattt tcatttacac tgttatatat atgaccatgt gcaggtattg gtacatatgt
     1561 gacaaacgaa catcttgtac agtatatttt tctttttcct tgtgtagtac aatcgggtgg
     1621 tgtagcacaa gtatctttgg tattgtgccc caaagcatcg gtataattat actttacagt
     1681 ataatcacag cctgatactg tgcagtaagc aagggtatag ccttttgcgg tacactttgg
     1741 caatacgacc ttagatttag tattgtgtcc tctcggatat atcatcacag tggatttgta
     1801 tccgcacttt ttgcataccc tgtatttaga gcctgtggtg gtacacccgg atttctgagt
     1861 aatcacatac tcgccgaata catgagaaca atcggcatat acagaataat taccgaaagt
     1921 tgcacaattg aacataattg ctaaaattaa aatagctttt aaaacgtttt tcataataaa
     1981 cctcctttat ctatattata ttaaacgaat tacaacaagt caatacaatt caggtaaatt
     2041 cagttaatct tactgaatat atgtcaaatt tttagatgaa atgtcaaaaa cagcctgacg
     2101 gatatcggta tccgtcaggc tgttacatat gattgatttt tcaatgcccc attatcttcc
     2161 catacagctc cggtcggcgg tcacggaaca cgccccagct ctgacgcagg tcacggttct
     2221 gctcgtggtc tattaccgct gttataactg cctcgtccgt tctgtcagcc tcggctacta
     2281 tcgcgcctgt agcatcggtt atgaacgatg agccgtagaa cgtcagcgat gagtcctgat
     2341 tttggttttc gggggtgggg tgaacgtact ccgtgcctat cctgnnnnnn nnnnctgttt
     2401 gcggcgatca caggcactaa gtttgcggct gagtgtccct gcatagctct tctccagtgg
     2461 ggcatcgagt cgcactccag tataggctca ctgccgatag cggtggggta aaggagcata
     2521 tccgcaccca taagtgccat acagcgtgcg gcttcgggaa accactgatc ccagcatata
     2581 cctacaccga tacagccgaa ccttgtattc cataccttaa agcctgtatc gcccggagtg
     2641 aaatagaact tctcctgata gaaatgatca tcgggaatgt ggctcttgcg gtatattccc
     2701 ataagtgagc cgtcacagtc tatcatagct atcgtattga aaaaagcatt gccgtatttc
     2761 tcatagaagc tgacaggtat aaccgtgccg gtttcccttg cgacctgcat aaaacgcttt
     2821 acggcggggt tatcctcagt cctgtcggca agataatagt aatcgtaatt cttctcctga
     2881 caaaagtacg gaagctcgaa cagctcgggg agaagtatca cattcgctcc gtccgacgcc
     2941 gcctgtctta ccatatactc tgcctttgct atattttctt cacggtcgtc actacagctc
     3001 atctggactg ccgcaacctt gacattactt atcataggga gtttcctttc tgacaaatga
     3061 cggtatctgg tgggttatgc aatggatatt tccgccgccg ataagtatat cgcgcgcgta
     3121 tatctggatt atctcgcggt cggggaatac cttctgtacc tcctgctttg ccctcaggtc
     3181 gtattcggta ttctcgccgc cgaacgcggg catcaccacg accttgttac cgatgtacag
     3241 gtttacatag cttgcgctga gcgggcttac ggtatctctt gtaggctctc cgtcaaacgg
     3301 gtcaagacct gctacctctt cctcggtcat atatatcggc tttggcattg caagctttac
     3361 aacgtctatc tttctgccga gtgcgtctgt gctgttttcc agtacgtcaa ggcaggcacg
     3421 gcacatctca tat
//
LOCUS       DAWAOG010000195         3409 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3588_length_3409_cov_2.206321, whole genome shotgun sequence.
ACCESSION   DAWAOG010000195 DAWAOG010000000
VERSION     DAWAOG010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3588_length_3409_cov_2.206321"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2960..2969
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          869 a          733 c          822 g          975 t
ORIGIN      
        1 cctcagaaga tgccgtgctt tcggcagatg aactctttga tgagctgtct gtcggctgtg
       61 aactgcaggc tgtcactgca catatcgata atgcaaggag cagtgcggta attgattttg
      121 ctttcttcat agatcgtatc ctttcacaga tatattcaag gcggcgactg cataaaatag
      181 tctgccgtct gccgcctgtt tattgaactt gaattaccgt attttactct attccggctg
      241 attgtctgac gcttttccag ctgttgtcgg caattacgta attatcgccg tttttctcgc
      301 cgtagggtat atagaaatcg gttatgctcg agcttgtctg ggcaagggta tagagaatag
      361 cctttatctt tgcgtcatag tcgccctgct cgatgtttgt atttgtaaaa ttgattataa
      421 aatcaatgta ttcggcaagc gtcttttcgc ctgttccgat gaagttctgg tcgatgaatg
      481 ccgaaatagc cgtcgcagat accttgcagg gataaagctc gtccttcaca ccgtaatccg
      541 gaagtgtaag ataagtgtac atctggtcgg ctgtaaagat ctgcgtcccc tgctttatca
      601 ccgtaacggt gttgtccttc ttgttctcgt actttatttc actcgggata gtaagcgttg
      661 tgtttcccac catatcaaaa agctcgctca gggtagattt tgtaaatcgt atgtatttgt
      721 ttattgtaag tccggtcttt tcggaaagct gttttgctac ttctgtttca cccgattgct
      781 cgcatatctg ctttattgtg ccgccgttaa gctctgtgcc gggaagatac ggcataagca
      841 caaaagtgtt tgctgtggca cggtaggtca cggtcatata tttatcggga actgtgtccg
      901 catcgttcga gagagtgagc agaacagaaa aattataaga cgagtcgggg gtaaaagcag
      961 tgccttgttg ctgtgaaaca tttacggtag tgtcgttctt ctgcgactcg taaaagctgt
     1021 ttagcaatat agttgataca agcactacaa gaacccctgt aataatgaag gttatagcgt
     1081 aaatgtacca gttgcttctt ctttttatca attttatcaa cctccgaagg cttgaaattt
     1141 actcagaaaa atagtataat actataatag cacaaaatga gaaaaaaaac aaggtgtatt
     1201 tttcggtcgc aggatttgca ttcctgctgc tttttcagtt actaatttat gtaaagaggt
     1261 tatagtatga acagattttt aaagcgtgcg tgggctgaga tcgacctcga tgcgcttaca
     1321 cataactaca atgagatcaa aaagcttgcg aagggcaagg agatacttgc ggtggtaaag
     1381 gcgaacgcat acggtcatga cgataaggcg gtatgcctta agcttcagga gctcggcgtt
     1441 aaatattacg gcgtatcaaa tctctgggaa ggcgaaaagc tgcgtcagca tgggataacg
     1501 ggcactgtat tgacattcgg ttacaccgat gaagactatt ttgacgaact gctcgaatac
     1561 ggcattaccc agactgcggg cagtgtgcaa tatgcaaagg agctcagcga ttatgctgta
     1621 agcaagggcg taaagcttcc tgtacacata aaggtaaaca caggtatgac ccgtgtcgga
     1681 attgacaccg aagaagaaat gctcgaaatt cttgctatgc ccgggcttga ctgcaaagcg
     1741 gcgtatactc actttgcggc ggctgacagc tttgaaccgt cggatataga atatacgaac
     1801 agacagcagg aaaagctgat gaagatagca agcgacaagg gactgcttat ccactcgcag
     1861 aacagcggag gcatagtata tcacggcgag ttcggcggcg atatcgttcg tgccggagtt
     1921 atcctctacg gtcactgccc gaactatccg cttgaagtgc cgtttgagat gaaaagcgtt
     1981 atgacggtaa aatccgttgt ttcacacctc aaaactattc ctgccggcac gcagataagc
     2041 tacggcagaa catataccgc cgataaggaa atgacagttg ccgttatacc gataggctat
     2101 gccgacggtt atttccgtga tttcagctca aagggaaaga tgctgataaa cggcgttctg
     2161 tgccctgttg tcggcagggt atgtatggat cagaccattg tcgatgtaag ccaagtacct
     2221 gatgtcaaga caggcgatat agtctatgta tattccgaca agtgcgagga aaccggcgtt
     2281 gactataacg caacccttat cggaactatc ggatatgagc ttacctgcgc ggtcgcactg
     2341 cgtataccta gagttgttat cgaaaacgga aaggtcaagg aagtagtacg ctacggactt
     2401 atttgagtac actttatgat atgagaataa aagctcccgc gggcatttac ccacgggagc
     2461 tttgtgctgt atatggtcat tgtatttatc cgggcttttt agctccgcag tttgagcaga
     2521 aattctgtcc gggtgcgttc ttactgccgc atatgcagta ccagtcgcta ttcttaggaa
     2581 cagtcgcatt cattgcatta cttgcaggct gacctgtcgg agctgtgttg ctctgtgcag
     2641 cagtctgata attctgttgc ggctgtccgt aatagttgtt atagttgctc tgataaggct
     2701 gctgaccgcc attctgacag ccgtaaccac cctgatatga gttgatattc tgataattcg
     2761 gcatattgta tgactgctga gccgccgtgt tctgcgcggc tgctgcggta agcgccttgt
     2821 cggcaatagt tttgaagcct atcacggacg cgaatattcc gaaagcagtt ataaatacgc
     2881 agaataccat agctacaata agacctgttg tgatcattga aagcgcaaag tctacagcct
     2941 cactcgctgt ggcaaagccn nnnnnnnnna cgcttcaccg ccgaaaaatc cgttgagaat
     3001 gccgagcgtc acgacgctta tcagctcaaa gaacgaaagt atcgccgtga ttattgttgt
     3061 tgtcataaca cctgtacgct tgcttgttgt aggcgtctgc ctgtactttg agcacagtac
     3121 aagcaccact attgccaatg ctataagtat caatgtctta aatctcctta tcgataatgc
     3181 ttaccttatg tgtccgcagt atatgcagaa tttattatcc gactcgttat acttgccgca
     3241 gcttttgcac tgccatgcgg tgttttcggt cgtaaagctc tgctgaacat ttgcggcaga
     3301 ttgtgcgcgg tatatctgct gtgcccttgc ggcgtttact ctttgctgtt cgctgtaagc
     3361 cgcggaggtg agctcattgt tgctcataca cttgtatccc ttgatacag
//
LOCUS       DAWAOG010000196         3409 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3587_length_3409_cov_2.403995, whole genome shotgun sequence.
ACCESSION   DAWAOG010000196 DAWAOG010000000
VERSION     DAWAOG010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3587_length_3409_cov_2.403995"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1073..1082
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          901 a          877 c          701 g          920 t
ORIGIN      
        1 gaccgtaaca gttacactat gatagcgctg tgcctcgggg aacgatatct tcatatccct
       61 cacagcactt cttatctctt cactgagcac tatagcttcc ttatcagtct ttacaggcag
      121 acaaacaagt atcttgtcgc tttcgtaacg gcaggttata tccgtgcgtg attttgatat
      181 tatcttgacg cagtttgcca cattcgcaag cacctcgtct gacttttctg tgccgtaaag
      241 cctgttgtac tctgcaaagt tatctatatc aacaagtaaa agcgcaacgc tgttacccgt
      301 ccttaaaagc tcagttgcct tttctatgcc gtacgccctg ttatagaggt ctgtacggct
      361 gtccatgacc gccgacatct tcattctctg cctgtcgtcc tttatccgct ttgagtttat
      421 cattcttgcg gtataaagac tgtacatcga gcaggatata aagaagccga gaagcgccag
      481 tccccacaca gtaaagggat aataacccga tacattgttc atacaacaga gtattgtgta
      541 ccacaccggg ataattccta ggaagaatgc cgatgcggca ggcgcaaaca ctggaactgc
      601 ggcaaatacc ataacagtca cagccgtctg tgaaagtccc gatgctccgg cggcgcaccc
      661 cgtaaccttt atacagtagg atataagata cagcccccag aaaacatatg gataatatga
      721 cgggtattta ccgctgaaat aaaagccgaa gccgacaccg cacactgcgg tgaacagtat
      781 cagaacctca cccgctatct ccgccgagcc gccgtcggtg attattccct caaatattac
      841 cgagaggaac gtacacaata tcattataag cgccgctatc ggtataatgc ggcagttata
      901 gcgtgaaata caagcatcaa gcaggctttt gccgctcttt ccctctatct tctgagtaga
      961 aaactttgcc gcagttgcca taggcaggcg gttctttatt tcgtttgctc cgagtgaaaa
     1021 caactctgta actttcatta ttttttattc cttccgggaa ttatcctcac cgnnnnnnnn
     1081 nntttttttt tttgggtggc agaacgggca ctcgtcataa tgctcaagct gaacgcttcc
     1141 gtcatcgccc gagtaatcct caatttcaag gttatccgta aacaggctct tacgctttct
     1201 gaagtaaata tagttaagca gtgatatgac acccgaaaat actacgctca ctattatatt
     1261 tctgacagcg ccgccgaatg tataaacctc aggattgacc ataacattaa gacaccctga
     1321 cataagctgt cgcacgccgt tgataacagg aacagagatc agaaaagcca ctatcagatt
     1381 atatcccgac actgcgagcg tccccattct tttgaataca agtacacaca gcacgagaag
     1441 ataaaccgtt accagaaaat ctgctacggc gatcgccgtg aataattcca tattcattcc
     1501 ggatatttcg gtaaatgcag cctgtatctt ttcgatggct tcgggattgt taattgtggc
     1561 ggctataaga atataaccga ttatacccga tatacagttt atgatattga gtatcatcga
     1621 cacatatctg aaaaatttaa accacttcag cggcagcgga taataatcca ttctgcgccg
     1681 caccatatct tttcttaaag ctttttcgtt gtttacgttt gtgttttcca ttttgctacg
     1741 tcctttcaat cactgaaaag gctccttctg tttttgaaat atcttaaatt cagaatgagc
     1801 catactgcca taaatactac tgagaaaatt gcgaaaagta tatttgttat gctgacttca
     1861 tagccgtcaa caccactgaa cagtgcaact ctgaaaagct cgcttgccga ggtgctgact
     1921 gcgtttaccg cagaagtata gatgaggaaa aatttaagga aaccgtaaga tctttgcgtc
     1981 agctcggaaa gctgtctgaa cattataacg caaagaggaa caacagccgc aagcagtacc
     2041 gacattacga tattgtatgc aataaagccg ggtattgcgg cggcatttgc acttttctgc
     2101 acgctctcgc tgaatgacgc aaataccgac acaacgcata ctatagccga gtagcccgac
     2161 ataacagcct gcaacaccgt tatcacggtc ttgaatatcc gaaaccatgt tatcgacagc
     2221 gggcaatcat acagcttagt gttcgcatag cgtattctgt cactgcggct gtttattaag
     2281 ggtttcttag gttcattctt gtacgaactg ttctttctct ttgcttttga catctcttgt
     2341 cggtttcctt tcatttgcct gaaataatca gattatttat ccgcctgctc cgccttattg
     2401 gaatgtgcgc aggttatatt gacaaggcac tttctcggac aggctgatac gcaagtgccg
     2461 cacgaggtac acttgtcata gtcgatcacg gcgtggttat ctgcaacaac tacagcgccc
     2521 cggggacact ttttctcgca tatcttacaa ccgatacaac cattggtaca taccgttctt
     2581 gtgaccttgc cgctgtcctt tgacgaacaa cgcaaagcga ccgtgcttgt ttcttttcgg
     2641 aggaatataa ggtggttcgg acaggcggta acgcactttc cgcagccgcc gcacttagcg
     2701 gggtcaacga ccgcaacacc gtctgttatc gtgatcgcat cattaccgca aaccgctgcg
     2761 cagtcgccgt atccggcaca gccgaaacgg cattcgccct tgccgttgta gaatttctca
     2821 acagcggcgc aggactttgt tcctgtgaat gtaaatctgt ccttagtcgc tttttcgcag
     2881 ccattacaac gcacataggc tacctcacgg ataaactctc cgccctcagt gcccattatc
     2941 gcgtttatct tcttcatgac atctgcgccg ccaggcttac aaaggttagt cggcgcaccg
     3001 tcatgagcta cagcggcggc atatcccgca caacccgaat agccgcagcc gccgcagttt
     3061 gcaccgggaa gcgcctctgt tatctttgcc tcggtttcgt ctgtctttac agccagcacc
     3121 tttgacgcta tcagcagtaa aatacccgaa agtgcgccga agccgagaaa tataagtata
     3181 ggtaaaatat attctttcat cgtctttcct ttcgcttaac cgccgaaaat tccgtcaaca
     3241 acaccgccga agcctatgaa agcaagcgat actatcgaag ccgccataag cgttatcggt
     3301 gcgccctgaa gcgccttggg tatgtcacag cgttcaagtc ttgagcgaac gcccgagaat
     3361 attatcattg ctatcataaa gccaaggccc gcaccgaacg agtttacaa
//
LOCUS       DAWAOG010000197         3396 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3610_length_3396_cov_2.304699, whole genome shotgun sequence.
ACCESSION   DAWAOG010000197 DAWAOG010000000
VERSION     DAWAOG010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3610_length_3396_cov_2.304699"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2840..2849
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1155 a          608 c          741 g          882 t
ORIGIN      
        1 ctcgcattac aaaggagctt gaagataaaa aagaactgaa tttgcagatt gtttcaattt
       61 tcaatatcgg ttatgtgccg cagggtaaat atgtaggctt tggctttggc aatataaacg
      121 caggctctac cgtatacaca gcttgtctta caatgaacga aaaggcagaa actgttgagc
      181 ttagcggaaa tgcagagttt gaaaatatac ctgcgtccga cagctcatta aagtcatata
      241 cgcttaaaaa agcgggtgaa atgagcagcg gagcaatatt aaaaatgttc agtgtgaata
      301 atgaaaccga gcaccttatt cttgacaaaa gcacccagac ttgtgatgtg ctgtttgacg
      361 gcgcggattt tgatacttac aatgttttct tttatgtaaa tcataagaga gtacaagtaa
      421 acggcaagga ctatgctacg gtatcggcaa aggcagggta tgtgccgaag ctcacggcta
      481 ttctcgaaaa tgtcaataac catgatattg tctatgctat agctgtgcct ttaactaatg
      541 attatacaaa ggcaagaata ccgacacaag tgcagatgta cccgaaagtg agcctgaaaa
      601 cagcaatacg gctgcatcag acagcgtatt gtataaatat aacgtggtag gatatattga
      661 taatgatttg aactatcttc tgttatataa aagagcgtca gatgaacccg aagacggtct
      721 tgaatatact tactctgtat atgataaatc aacaggcaag ataaccgaaa gcttaagaag
      781 ccaaaaatca ccttgggata tttcctgctg taacggacag tttacctata tagatgagaa
      841 cttgaccgaa gaccaaagcg ttacgtcaga gatattcgcc gtaacatata atgaaaagtt
      901 tgaaaaaata agcagactca gcggtcttac ggtaagcgat agtattcatt atatatccgc
      961 cgtatactca aagcagcttg ataaatactt cgtatgctat agaaaagacg gcagagacga
     1021 atacataata agcagattca gcaaggatgg aaaagaagaa aaggagcttt tcagcgacag
     1081 cgattactat tatagcagta caatgctgct tgcggataac agaattgtcc tttgtaagta
     1141 tcgcgcagta ccagacggtt gctcaatcca ggttatagac tttgacggaa acgaaataac
     1201 cgccgatatg ccgcagagta ccgattatta tgcgctgatg gggcaaaaat cgggtaaata
     1261 ctactgcctt ttatctaatc gaaccggtta cgcatccaaa accgaagagt tccccgagga
     1321 tacattgtat ctttatgacg gcgaaaagga caaaatagtt gaattcacac cagaagaagc
     1381 tattgaaatg ggttgcgtga gaataactcc ggacggtaca aaagcggtaa ctataaccga
     1441 acatgagttt ttgaatgagg agcagacaaa tcgtctgtac accgatcttg taataaaaat
     1501 atatgacctt gataccggag aaaagataaa tgagataaaa aacgacaaga gcagcggtgt
     1561 aaaggggcgc aacattcagg cattcgatga tagaattgtt gtatatgacg gcagcattac
     1621 agattatatt ttatttcagt tcagatatga tgagcaggcg ggataagtga aaattgttgt
     1681 taatcggcgt cggacaattt acataaatta tttagattaa acggaggaca tttaaaatga
     1741 ttaaaaaact tttacgagca tcagcggcat cacaattcgc tatagctttg gtcctctcac
     1801 tctgctcctg tgcgggaaca agtgaaagtg acacacagag ccttgtgtca aatccatttg
     1861 aaacatcgga gaacctaaac agcgaaacac aagtgagcaa cccgtttgac acaacagctt
     1921 ccgtaacaaa tatctccgta gattcaacgg ttatagaaac attgcctccg tttagtaaac
     1981 ctcaggaatc cgaaccggaa aatgatgatt tcagcaaata tcaggaagcc ggaattgcat
     2041 tcagcgacta cagcattgaa ggcaagtcaa ctgtaaagta cagcggcggt gaggtaaatt
     2101 tcagctttgt gtccgattct gcaaagaata aatatgatgt agagattggc tatatggcat
     2161 ttataaacgg tgtgcctcaa aagctgtcgc tgaacggctc tgaaaaatct gagcttgtaa
     2221 gtatttcaca aaagcccgac acaaagcaga cggttactct tacctttatt ccggagataa
     2281 caaaagaata cgaacatgaa aaaatattga aagtaaagct gatgaccgtt ttgcacccgt
     2341 cttacagacc taaagacggc tttttaggct tcggtaacgc acattccgga cagtcgttct
     2401 ttgagtttga gattgaagca gacacagaac cgcagattat cgacgatttt acatattcgt
     2461 cagattgtga aagcatttta gcaaccgcaa aagtaaaaaa ggagtataat ctgactgacg
     2521 caaaaatatc cggcacaaat atgataagtt tgtttgatgc aaagcgtcag gaacacagct
     2581 tatccattga taaaagctcg gataaattgg gcgttactct gcttttatat ggcggcgatc
     2641 ccgatgaata tgctgtatat ttttataaga accatgagcg aataaagctt ggagagaacg
     2701 gcggtatgag atttgcttca aaaaccggtt atataaatga gtacaaggtg acgcttgacg
     2761 atgtaaaagc gggagatata atttatgctg ttgaagtacc gctgaacgcc gaggttggct
     2821 caatgaatat ccgtaaaagn nnnnnnnnng tattagtatt gtggttacgg aaaacgaata
     2881 agatgacaaa atatatttca taaatatcgg aggagcacaa atgaaaaaac gcattatcac
     2941 atctgcactt gctttggcac ttgctttctc actttgtgct tgtatagaga atacaccgac
     3001 aacggaaagc tattccgata gtacaagcgg aacagtcgta tcatcagaga cagcctctat
     3061 tgcacaaagc gatgaaagcg ttcttttacc ggagagtttg cccgaaacga gtaaatcaaa
     3121 tcaggctgac gaagacgtag aaataataaa catttatgac tatatccctg tcggatatgt
     3181 tgatgaagag cagaggtatt tgctgctgaa agcaaacgca gataactcct taaacgatta
     3241 tacttgtgat tatgtagttt atgacgatag agaaaagaag gctgtatatt gctttacagt
     3301 cggaggttac tatgatccgg catctgaatc acagcctttt gattcggatg taaaatatag
     3361 tgaaggaatg ttctcttttt ctgcgtggaa ctatta
//
LOCUS       DAWAOG010000198         3390 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3620_length_3390_cov_1.903748, whole genome shotgun sequence.
ACCESSION   DAWAOG010000198 DAWAOG010000000
VERSION     DAWAOG010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3620_length_3390_cov_1.903748"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    548..557
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1323..1332
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1652..1664
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2857..2866
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1012 a          734 c          720 g          881 t
ORIGIN      
        1 ctccttgtct gtagcaagcg ttgcggatac aagtgacgct tcctgtgccg ccgttataac
       61 cgtcatcaca cttgaaaacg taaacagcgt tataagcgtt attaccgccg ccaacttttt
      121 acttgctttc atctttttta acataaccca cctccgtaat tatccgtttt ggcttttttc
      181 gtttattgaa ctttcgattg ctttttcaag ccccgtttcc gcttttgcga ttatctcatc
      241 gataagcctt tttgctaccg agtgcagatc aaacgcaagt acagcttcta tcaggctcgc
      301 acgtttttcc ttaggcacat catacagctt aagacagcac tcaagaaaac gctctatctt
      361 cttttgcacc gtgaagtaca tatcacattt cctagccata aaaccacgca tgatacttcc
      421 cgacgctata tccatctcgt agaaatcctt atcctccgca tccggcataa acggtgagaa
      481 tatagccttt atctgcactg ctgtactttt taaaatatat tctgtagtgc tcggcaacgt
      541 atatgccnnn nnnnnnngta tatgccatta catatatctc cctcagcgga tcggacagct
      601 cggttatata tatctgcaat gaggtttcaa ggcagtacac cataagcggc tccatatcct
      661 ttgcaaatgt cctcgccttt gcaaactgat tttcaaacat aatctgcgta agcgtcagaa
      721 gcaatgcttc tttattttca aatgccgcaa aaaacgatgt cggcgacatt cctgccgctt
      781 ttgatatctg tgcggtggta gttttttcat atccctgctc taaaaacagc tgtattgcgg
      841 caagtagcat tttgttacga cgggctacgc ccttcggttg taatcctttc aatttatatc
      901 ctccttattg ccatctatac tataattgaa agtctggttt tgagtttact caattatagc
      961 atattgtttc tcaaaagtca ataactaccg tattttttat tcaaactcgt ttatgcaaaa
     1021 cctacaaaac tgataagaat taaacaaaag ttttcggcga actaattcca ctaaacctat
     1081 tgacaaacta ggtttagtgt gttataatca taacaacgaa aacgaaaggg gctgacgaaa
     1141 aaatgatgag cataatgcga atgtgtaaag acaatataat aatgcgtctg tcccgcaaga
     1201 acatctgagc ctctcagaac ttcaaagcgc acggacgtta agcaaaacgc gtccgttatt
     1261 taatgggagg atatatgata gagcttaagg atcttgaaaa gacgtaccgc acttcaaagg
     1321 gcnnnnnnnn nntgctgttt ttttaaatct ttgccggtat atacggtgag atattcggta
     1381 tcatcggact ttcgggagcc ggcaaaagca cactcgtacg ttgtataaat tacctggaaa
     1441 gaccgacatc gggacaggta ataatcgacg gcaaggagct tggaaggctg agtaacaagg
     1501 agcttttaaa aaccagacag aatataggaa tggtgtttca gggctttaac cttcttgcac
     1561 agagaaacgt tataaagaat atctgctatc cgcttgaaat cgcaggtatg aaaaaggcgg
     1621 acgctgtgaa gagagccgaa agcctgctgg annnnnnnnn nnnnctttcc gataaagcaa
     1681 aagcgtatcc gtcacagctt tcgggaggac agaagcagag agttgcaatt gcaagagcgc
     1741 tcgccgccga aacacactat cttctgtgtg acgaggcaac aagcgcactt gatcccgaca
     1801 ccacaagatc ggtacttgaa cttctgaaaa agataaaccg tacgctcggt gttacgataa
     1861 tcgtaattac tcacgagatg aaggtaatag atagcatatg cgacagggtc gcagtacttg
     1921 acaacagcac ggtcgcagag atcggcgatg taagaacggt atttgcaaat ccgcagtcgg
     1981 atatagcgag aaagcttatc atgccgcagg gcggacttcc tgtaacaagc gagaacggaa
     2041 agaagataag aattatctac aatggcgaaa actcatcaaa gtcactgtta tcggatatga
     2101 tattaaagtg caatacgccc gtcaatattc ttcacgccga tacgaaagac attgacggca
     2161 aggcttacgg tcagatactg gttcagctgc ccgatgacga aatttcggca gaaaagataa
     2221 cagcatatct taaagccaac agcgttacct attcacaggt gtaaggggga agaaaaatgt
     2281 cagacgatct tatacagctg tgggataaag gcgtactttt acagggtata tgggaaacaa
     2341 tatatttaac gttcatctct tcatttatag cttacgtttt cggacttccg ataggcgtac
     2401 tgcttacagt gaccgataag gacggaatcc accctatccc gtggctgaac aaaataatcg
     2461 gtattttagt aaacatcttc cgcagtatcc cgttcatcat tctgatggtt gcgtgcttac
     2521 ccgccgcaaa ggttattgta ggaacaagtc ttggcaataa ggcgatgata gtaatgctgg
     2581 tgatagcggc tatcccttat gtggcaagaa tgacagagtc ctctttcaaa gaggttgaca
     2641 aaagcgttat tgaagcggct caggcaatgg gcagctccac attcaagata atatacaagg
     2701 tgcttatccc ggaagcaaag cccgcgctta tggtcggcgc agtcatttca ctggtaacta
     2761 ttctgggtta ttcggcaatg gcgggaacga tcggcggcgg cggactcggt atgatagcta
     2821 tctcattcgg ctatcagcgt ttcaataatg atattgnnnn nnnnnngata tgcgtactgc
     2881 tcacggtcat catagtacag ctcattcagg agcttggtat gctgatagcg cgaaaggcag
     2941 acaaacgtat aaacaagtaa ggagagatat aatatgtcac tgctttttac aaagctctta
     3001 agacagaatt tccgtttcgg gcgaatgtgc ctatgttgta tataacagat accataaagg
     3061 tgcagaacga agaaagacaa ataaaaatac aacaatggtc ataaccgaca gatataaagt
     3121 cgggcaggcg gtaaaaccgc agaaacggag aactaacatg aaaaagatat tatcactgat
     3181 actcacagcg gctcttgcag gcgcaacact tacagcgtgc agctcaagcg gcacaagctc
     3241 aacagcttct tcaaacgatg cttcatcggc agatacatct tctgcagcat caagcgccgc
     3301 agaaagcacg gcagacacag acaacgttat caagatcggc gcaacagcta caccgcacgg
     3361 cgagattctt gaatttgtaa aggataagct
//
LOCUS       DAWAOG010000199         3387 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3625_length_3387_cov_1.882653, whole genome shotgun sequence.
ACCESSION   DAWAOG010000199 DAWAOG010000000
VERSION     DAWAOG010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3625_length_3387_cov_1.882653"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          861 a          828 c          657 g         1041 t
ORIGIN      
        1 atcatattgt tatcgccgtc gtcccatgta aggtcggcat gataccattc accgtcaagt
       61 tgcaccatat tccacatatg cggctcatcg tcactcagac cgactaccaa tatgttatct
      121 accgctatac attggcaaag gtactgatac gctcttgaat atgactcgca aacaccgctg
      181 ccggtattaa gcactccgaa cgcttcatac gttccgttgc ctttgagaga atagtcatat
      241 tctgcgcttt taagtatctc gtcatgtatg tcaagcagat aatcaacaag cgattcggca
      301 ccgtctgctt tagcgagtat ctgctccgct ctgttttccg tttgctgtac atagtactca
      361 cattcggatt tgttgaagtc ataatccagt ttaattgagc gtacatcagc cttactgccg
      421 ctccagcttc tgaacgaaag cggcgcttga aaaagcagcg gattatcgta atttacacac
      481 atcaatattc tgtaaagccg ctgatatgac acgcctgtaa acggcacatc aaaccgtgct
      541 tcaaaattac tcacagctct gtaaaggcag gaataaatat atctgtcata cttgtcaagc
      601 gaattatacc catatccgta aaacgcatct tccgttgtac cgagtatcac ataagatcca
      661 acgtacacgc ggctcatctt gccgtccttt tccgctctga cgcagatatt cgttacaccg
      721 gtttccagtc tgataccaat ctcgggatca taaagataag cggttttatt cttttcgggc
      781 agcgttccgt ctgtggtgta ccatattgta tcattgcaat caaatttcag cgtctggggc
      841 ttgtgataat atcccgattt gaaattcgga ttaaattcat actcaaagcc gttatcaaat
      901 tccttatcat caaaagaata tttaaacgga agctctgttg gacttctgag ccgttctctg
      961 aaataatcat aatcggtttc ttcaatatct ttcgccagat aataagaaac tgcatttttc
     1021 acactgcgct ttgaattatc gggaaaaaca aacgagaaaa caacggcgat cacgccaaga
     1081 ataaaaaata ccgcagctcc tctgctcata cattcacccc ttatatttaa tcgtttatcg
     1141 tctggtttta agcttcattc tgatatcatc accgctgaaa acaagcgttt tcatagaata
     1201 aagccttgaa actattcggt cggcataacg ttttttcagc tctgttcccg taagatttgt
     1261 attgactatc gtaggtctgc cgtaattcag ccttgtgttt atcagctcat aaaggcacga
     1321 aacgtaaaac tgcgactcaa actccgcgcc aaggtcgtca agcagcaaca ggtcgcactc
     1381 cttaagcgtg ttcattacct cagcatcgtc ctcagtctta ttaaagtgag cgttttctat
     1441 ccgtcttagc agatccggca ctgagccgta caccacggaa tagttctttt caatgacctt
     1501 ctttgcgacc gacaaggaaa ggtgcgtctt tccaagcccc gttccgcctg tcataaaaat
     1561 gctctcaccc ggcacaagaa aatcctccgc atacgaaacg cagaagtcgt aattctgctt
     1621 cattatatct cttggtgatt tattcttaag ctctgccttg ggctggtcac tataaagact
     1681 gaggtcgaag ctctcaaagc aggtaagtcc cataggcagg ctcttactca gctcccttga
     1741 cgctatcatt ttcagcacat cgttatagca tgaacatctg ccatgttcgc cgataccggt
     1801 atcacagcac tttttgcagg tgaatatcgg gtcaagatag tcctcggggt atccgttgct
     1861 tttgagcagt gttctcattc tgtcctgaag cgcaagattt tcattcttaa gcctttcaat
     1921 tttttcacgg gcatcggaat ttcttgaaag tatgattttt gccagcacat cgatagtacc
     1981 gctgagcttt ctgtcgatat cccctatttc gggaatttcg cggcgcagct ctcttgttcg
     2041 tttctctatc gtgacgctgt tgtcaaagcg gcgcttgtcg ataatctcct gtgcttttgt
     2101 ataataattt gacggataag ccatatttac tccttatgta taatcttcaa gtagcatatc
     2161 atcgattttt tctgcgtcaa acgatgaatt attctcggta gagatcggtc tgctgttctt
     2221 ttcgtcattt acctgctgct cagttgttat tccctttgtg tgccagtttt caagtatctt
     2281 gctgatatac gggaaagcga actggccctt tgcgttgacg ttgatctcgt aagccagctt
     2341 taccatttcg gaattgaaat tccacacgtt ataccataaa tttacaaaat ccttttcctt
     2401 ctgcgtcaat gccctgttta ttccgaacat agacttgacg gagctttcgg cgatgttttt
     2461 agattgcagc attgatatct gtctttctgc ggcggcaagg tcgtttatgc cgttttccat
     2521 ccagttcatt gctgtttcct taagatatgc gggcgatgat ttgtttatcg agaagcagta
     2581 atccaccatc ataagtgcga cctcggtcgg aagcccgata tgctcggtga tcgttataag
     2641 tgcgttctgc tcggtgtgct taagcggtct gccgtaaagc atttcgcagg tcttgaaaag
     2701 aaaatcgacc ttatcgtctt tccttaccgt gtccgcgatc tctgcaggtg aaaattcggg
     2761 tgaacgtaaa gctgccgcct ttactgcggc gaattcagcc tgttgcgccg ttacgccctg
     2821 cggaactgtt acgctcgccg atacaacaga cataacaggc tctttcacct gcggctttga
     2881 agcgggcgct ccctgcttcg gttcgtatat gccgtgattt tgcgataaca gtccgacctg
     2941 ctcccagaat gaaagcgcgt cctttacgtc ctctgccgcc attgaaagcg cctcggatat
     3001 ctctgtgtca ctgagctgtt ctccgctgtg acgcagaaaa tacagcaaaa ccttgatact
     3061 gctcccgctt gctatcttta tgtatttgtc aacgacatct gagggcactg caaaaatact
     3121 gccccaactg ccgaggttta taatataact cataattgct ccgtttttac ctgttttttg
     3181 atttaaatat accttataat tataccacag ttgacacgaa attgcactac ttttataaaa
     3241 aaactctata atttgctgtc gtttgttaag acaaaaaatg agcaccgata taaaaccgat
     3301 gctcatttca gactgtagaa aaagtctatt tttataaaaa tagagcaaat tctctaaccc
     3361 taaccccaaa ccccttcccc gtgggaa
//
LOCUS       DAWAOG010000200         3350 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3671_length_3350_cov_2.399697, whole genome shotgun sequence.
ACCESSION   DAWAOG010000200 DAWAOG010000000
VERSION     DAWAOG010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3671_length_3350_cov_2.399697"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1122..1131
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2301..2310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          927 a          635 c          749 g         1019 t
ORIGIN      
        1 acgacggttc gtttattaat cagccgcttt atgcgattga agaatacaga tataccggca
       61 gaagtgattt tgaggttaga aaaaacgagc ttgtaaaaca gctttataca ggcaggcgta
      121 tttccgcata cggaaactat gaggcgttca ggctgtactt tgattacgag ttttcctcgc
      181 ttgtcattat agtgatgatt atttttgctt ttgcggcgtc gctcagcgat gaaaaagcga
      241 caggcacgga caggataata cgctcctgcc gcagagcaaa aagcgtattc cttactaagc
      301 aggcggtaat gctgcttttt attaccgtgc tgatagtttt gtttgcggtt atggatatag
      361 tgaggtttag ctcgttttac gacggttcgt ttattaatca gccgctttat gcgattgaag
      421 aatacagata tacgccgctt aatgtcacgg tattcggtgc tgttatgctt tccttgcttg
      481 gaaagctgct tgcgcttgtt tttatcggag aaatcgtact gctgatttct tcgtttacaa
      541 aaaatgcggg attttcgata acgctatgct ttgcggctgg cggagcgatg ataatcataa
      601 gcgagcatat aaatccgctg ttttctccgc tttcgctttt aaacgcggaa agctggatga
      661 aggattttaa ctgcgtcaat attttcggct atcctgtact gagctttatt gttcagtttg
      721 ttatcacggc ggcactgatt gctttgctga ctgctatctg ctatttcaga gccggatata
      781 taagaaaaga aaagggggcg gtacagtgat atttttatat gagcttaaaa agatactgat
      841 aaagcaatat tcgctgttat tatatcttat agttattatt tttgccgctt tttctcttat
      901 tacggcacag caccagactt acggctttgc aacggtcacc gaaaggaacg agtaccttga
      961 aacaatcaca ccgttcacag gtgaattgac tgatgaaaaa gcggcggaga ttaccgcact
     1021 taaagaagaa tatcttgccg caaaggccgc tgtaaatgag ctaagcaata agtacgcaaa
     1081 cggagagctt gacgatgccg cttttgagcg agagatcgca cnnnnnnnnn ntccaatatg
     1141 gaataccgca cggctttaaa tcagatgaat atgcagcggc agaccccgaa aagcgtatgc
     1201 tgatacccgc cgggagcgta cctgttctca gcgacacatc ggtaaacttt ctttttttac
     1261 tgtgcataac attctgctgt gcgtatgcag taatgataga atactccggc aaaagcagat
     1321 acatacttat cacgacaaag ggcggacaaa aagcgactat ggcagcaaag ctcgctatac
     1381 tgcttttgct tacctcgctt acaagtataa taatatcctg ctttgatctt gtgcggctgt
     1441 cggacgaact gccattagag tattggggat atagcttatg ctctgttcct gcatttgaca
     1501 acaccgcagt agatataagt attatcggcg catttttatc cgcacaactg ttaaagctta
     1561 taggctatct gtttatttgc attgccgcca tgcttaccgc tcattttacc aaaaactatg
     1621 tggcgagcgt gttcccgttc atagctgtgc ctgtggtagc cgattatctt gcggaaagag
     1681 acagtcaggc tttgttcttg ccgacgggac tgctaaaagg cgcaggatat ttcttaggtg
     1741 ataaggcgga taagggatat gcgacagaag ccgatacggt tatatatttc catcatgttc
     1801 cgcccttata tacggttatt atgactgcgg cggtcgtatt gtttattgtt atcggtgcgg
     1861 ttattctgat aaagtcgggc gagaacaggc ttaacagcaa aaggcccgga gtgaagcata
     1921 taccgaaggc agttgttctg acacttgctt tactgttttg caccgcctgc ggaagtacac
     1981 gacaggcaga atgtgttgat gtcctctcaa acaccgagaa cagccgatat tcttttgagc
     2041 tgaaatatga agacgaaaaa gagcttatcg cagtcacgga taagtcaagc ggagaaagct
     2101 ttattattcc cgaggagacc tttgttgatg aatcctattc catcatgtct atttatccga
     2161 ccgagaacta tctgtattac atgaagggtc gtaactcagg ggtttcgata taccgcataa
     2221 aactgtccga tatgacaaga gaggaaataa taaaatcgtc agatactgcg gataaatcca
     2281 tactcggact taccttttca nnnnnnnnnn ttcatggagt gataccgaaa acagattttc
     2341 ggctgtccgc ttgttcacag atgaaaagaa tatatatctt atagataaaa acggaatggt
     2401 ttttgtgctg aatccctttg ccgagaaaat aaccgactgc acctgtatta ttgaggagag
     2461 cgtgctcggc ggatgtattt ttgacggtaa agctatctat tatattgatg aagacggcgt
     2521 gcagaaaatg tacaatgttg tgaacaagac atttcggata taatcattta agatatgagg
     2581 agaaatatca gctgatgtta agattattcc tcgggtgttt agttttaata atttctttct
     2641 gctttgcaga agtaacatgg gtgtatatct gcgttgtagt tatagaacta tgccctagta
     2701 atttctgaat atatctgata tcaacatcct gttccaacaa gagcgttgca aaagagtgcc
     2761 tgaacatatg cggggttata tgcaaagata taccgcacgc ttgacaataa gaattaatca
     2821 tacagcggac ggattgctct gtcagtcttt tgccatattt gtttacaaaa aaatatccgc
     2881 agtccgagat tttttcctca tagataaagt aataatcgga aagaatagtg agtactgagt
     2941 tgtgttcaat aggtattata cgttcttttg agcctttccc gtaaagctta accgatttgc
     3001 ctataaaatc aacatcttga gagtgtaaat tgcagatttc tgccactctt gctcctgttg
     3061 caaacagcaa ttcgagaacc gcggcattgc gtattgcgct gtttttctta tattcggtgt
     3121 ttgatttttc tatttgcgta tatgcaaaac agagaatctc gccaatagtg tccaaaggaa
     3181 tggtttttgg cagcataaca ggttttttga ttgatgtttc gattttatca aacggattgc
     3241 aatcaataat atcccgaata agcagatagt gagcaaacgc tttgagcgtt gccagctttc
     3301 gtttaactgt tttgggttta taggatttat tcaagtgctc tatgtagttc
//
LOCUS       DAWAOG010000201         3347 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3675_length_3347_cov_2.227217, whole genome shotgun sequence.
ACCESSION   DAWAOG010000201 DAWAOG010000000
VERSION     DAWAOG010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3675_length_3347_cov_2.227217"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1326..1335
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2875..2888
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          952 a          667 c          790 g          914 t
ORIGIN      
        1 gccgccgaca gcgggctcat caatagcaac aagcccgaca gccaggatat ctgcttcgtc
       61 cccgacggcg attacgccgg ctttatcgga cgctatacag gcaaggctta ccccgacggc
      121 gatttccttg atgaaaacgg caacgttata ggcaggcaca agggcgttat attctatact
      181 atagggcaga gaagaggcat aggcacgggc tttggtaaac cgatgtatgt tatcgggaaa
      241 aatgcccata ataataccgt gaccctcggc gaaaacgagc ttcttttcac cgataccctg
      301 tatgcagacg atcttaactg gatagcgttt gaatatcccg aaagcgggtt taagtgcaag
      361 gcaaagacgc gctacagaca gactgaacag ccgtgcacgg tatatcctca ggataacaat
      421 acggtcaaag tagtgttcga tgaaagaata agagcggtaa cgcccggtca gcacgttgtg
      481 ttctatgacg gcgatacagt gctcggcggc ggtgtaataa tgtagaattg tgaaactttg
      541 acaaaaaacg agttcacttt tcatctaatt tacacaaaac tattctttta atttcacgaa
      601 caagcattat tatataacta cagtcagcga gagagcaaaa cgacagcagg aagatcggct
      661 gaggataagc gctgacattt ataattgcta tcttcgattt tcatatatat ttccccaaag
      721 caaaggactc cggtgtaaca gccggagttc tttgtgtttt atacaggctt tacggtatta
      781 ttgcgttgcg aatgtttcgc ttgtctttca attgtcgaca tacaatcact agccaatgcc
      841 gctgttaaaa ttgtactaag gtacattgta atatagacag tatagcatta cagaaataga
      901 acaacagttt ttcgcaaatt ctgaaatagc ttgcttttcc gcaagccatc acctccccca
      961 tactcgatta aattattttc tcaaccctat tgacaaacgc ataaatctat gttattatac
     1021 aactgtacta actcaaacag tacagttatc acgaaaggcg gtgcagtatg ttttctttaa
     1081 gacttcaagg cggaatacct ttgtccgaac agcttgaagc aaggatagca gagctgataa
     1141 tcggaggaaa aatggcggaa aatgaaaagc tgccggctgt gcgggaagtc gcaaaagcgc
     1201 ttacgataaa tccgaacact gttcagaaga cctacagaca gcttgaacag agagggctta
     1261 tatattctct tccgggaaag ggcagttatg ttgcggagcg ttcacaatat tcttctgccg
     1321 tgttgnnnnn nnnnntcgtc aggctgtgat cgatgcgaaa aaatcagggc ttaccgaaga
     1381 gatgatgact gaagtaatta acgccgtttc aaataaggag ggtgaaatat gataaacgta
     1441 gaaaaggtaa ctaagaaatt cggcgatttt cttgctcttg acgatatgac gcttaatgta
     1501 aaaagcggca gtgcctatgg actgctcggc tcgaacggtg cgggaaaatc aacacttctg
     1561 cgtattcttg cgggaatata caagcaggac agcggaagtg ctacggtcga tggggaaaag
     1621 atctatgaca acgtggcggt aaaacagagg atattctatg taagcgacga tgttgagcag
     1681 tatgctaata tgacccccga ggatatgaga aaaatgtatg aggtattcta tccgacattt
     1741 tcaaaggaga agttcgacag acttatgact gttgtggatc tgccgcgcga caagcgtatg
     1801 tctacattct caaaaggtat gaagcggcag gcggcggtta tatgcggact tgcggcatct
     1861 accgatatcc tcctgcttga tgaagcattt gacggacttg acccgacaat gagaattaat
     1921 gtcagaaaaa tgctttttga cgctatggcg gagaataaca gcacggttgt gatttcttca
     1981 cacaacctta cggaaattga ggagctttgc gacagcgtgg gtcttgttca taaaggcaag
     2041 gtggtatttg accgtgagct tgactgcata aaaggcaatc tgcataagat acaggtttcg
     2101 tttgacggcg taaagacagc cgctgatttc ccggagcttg aaatcctgtc ggaaaatacg
     2161 ataggcagtg tacagagctt tattgttaag ggtgagcctg agaatgtctg ccgtataatg
     2221 agcgaaaagg gtgcaaaatt ctgcgatgtg ataccgctca cgctgaacga aatattcata
     2281 tatgaaatgg agggacttgg ttatgacagc tcagaactcg ataagtaaaa agagctactt
     2341 cggcagctat ttcagataca agctgttcaa aaacaaaaat ctgcttattc tttctttgat
     2401 attcagcata ctcggtatac cgacatttgc cgtctgcgtt tctgtttgta ccggaatact
     2461 caacgacttt ataaatgcag gtgtattcaa tttggataat atacaggaaa ctctgggcac
     2521 tgttgatacg attttgatgt ccggtgtcat agtatcggtc gtgtcggtcg tgatatttct
     2581 gataatgcca tttgtggtat caaacgtcat attcggctac aatcttaaaa agtgtgacgc
     2641 agatatgatt ttaccgcttc cgctcggtac tacccaaaga ttttttgccg acctgctttc
     2701 gggttctgtc atatgcattc ttcccacact gctgacaggt gcggtaagcg cagtggtaac
     2761 ctctgcaagt aaaatcgcgc tatcggacaa gataacgcaa atatataaca acggtaactg
     2821 cactgcggat atagatgcgt tttatcgtgc gacaaatttg atagtgtata aggannnnnn
     2881 nnnnnnnntt gttattgttc aggcgcttat gtatgctttc tgcgtgatga taaacgcctg
     2941 cgccggtaga acggctgaca gcattatcta ttccgtactc ggtatggtgg tattgtcaat
     3001 ggctgttgtt tcgtttgccc gcataatcat gggcagactg accagtgttt caaacgatga
     3061 acagattttt tcactggcaa tgtatgccgc accgctcggt cctgtttttt cgttcatcgg
     3121 ttctttcttc aactcatata agtatgttga atatcagatg gagggaacgt tgtggcagag
     3181 cgatatgttc tcggtgttca gtgtgcgcaa tataacggta cttgcagtac tgttcttaat
     3241 ttatctcggt cttgcttatc ttgcaacaag gctgagaaaa gctgaaaaga caggctcgcc
     3301 gttcgtgttt gaactgccgt atcatataat aacactgggt cttgttg
//
LOCUS       DAWAOG010000202         3340 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3690_length_3340_cov_2.405784, whole genome shotgun sequence.
ACCESSION   DAWAOG010000202 DAWAOG010000000
VERSION     DAWAOG010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3690_length_3340_cov_2.405784"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2173..2182
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          991 a          622 c          823 g          894 t
ORIGIN      
        1 ataggcaggg tgtcccgaaa cagttccgtt ttttcaaaag aaaattacaa aaaattgtcg
       61 acaaaacttg acaactgtgc cgatatcgta taatatatag ttataaccgc gagaaaagag
      121 atatttatga ccaaaaagga aagagcaagg cttgttattg atgtgcttgc tgagtgttat
      181 cccgatgtaa aatgtgcgct taattatgaa aaaccgcacg aattgctgat agcaacaagg
      241 ctttcggcac agtgcacgga taagagggtt aatcttgtaa ctccggcatt gtttgcgagg
      301 tttcctaatg tagacagttt tgcggcggct gagcctgacg aggttgaaga atatatacgt
      361 tcctgcggac tgtataagac aaaggctgtc gatatagtta tgatgtgtcg catgatacgc
      421 gatgagtttg acgggagagt tccggatacc attgagcagc ttgttaagct gccgggagtg
      481 ggcaggaaaa ccgcaaacct tattgtgggc gacttgtacg ggaagcctgc gcttgtgtgt
      541 gatacacacg ttataagggt cagcggcagg atcggattaa cggacggcac aaaagatgca
      601 ttcaaggtcg aaaaacagct ttctgcagtg atacccccga aagacggtct tatgctttgt
      661 cacaggcttg tatggcacgg cagacttgtt tgcggcgcga gagcaccgca gtgcggcgaa
      721 tgcaggcttg gtgcgtattg caaattcttt tcgtgtaaaa aatgatataa cggcttggag
      781 tgagtgttat gaaagggaag gtgcgtaatg cggtcatcat cggcgcagga gtacttgtcc
      841 ttaccgtaat gctcactgtt gttttatatt attatgaaga taagtcaaaa cgtgataacg
      901 catggatcta ttctgcggcc gaggtgtcgt cctcattcga gaacattaac acgaaaaaga
      961 cagacatcaa caaagtcgat cttgatacgc ttatgcaggt aaaaggtgtc ggcagacaga
     1021 tagctcatga catagtcaac tatcgcgata aggtcgggaa aatttcttct atgtatgagc
     1081 tgaaatctat acccgctgtc aatgatgatg tgtatgagat actgtgtact cagtttaccg
     1141 ttacaggcag ttccggttac tacaaccgaa acgagtcata cggcgttgca aaggtcaatc
     1201 tcaataatgc gcagataaat cagcttctgg cggttgaggg agtaacacag gaactggctg
     1261 aaaacattat tctttaccgg aaaaataacg gagattttac ttcggtaagc gaactgcttg
     1321 atgttgaggg tatgagcata acgttataca acagtatttc tgataaattc acagtgtgaa
     1381 atgctccgaa tagcaccaga aactattgat tttaaagtgc ttttttgtta taatatgata
     1441 aataaagcac gaaaagcatg aatacatttt taaaaatcaa gtaatatatc cgttatggag
     1501 gtcagtatgg attataatat caaagatgtt tccggcagac tgaaaaacac aagagaatat
     1561 ctcgatattt ctgtcggaga gatggcaagc aagacgaacg tttcgacaga agaatatgtt
     1621 tcgcttgaga acggagaaaa ggatttttca ctttcgtttc ttaatcaagc cgccaatgct
     1681 cttgggatcg agcttataga gcttcttacc ggagtaacgc cgaagctcaa cacatattcg
     1741 atcgtacgca aggataaggg acttcctatt gagcgccgtg aaaggtttgc gtatcagcat
     1801 cttgcttatc tttttaagaa taaaaagatc gaaccgttgc ttgtaacagc gccttatgat
     1861 gaggaagagc agaataagcc gatatatctt tccgtgcatg acggtcagga gatggattac
     1921 ataatatcgg gaactatgaa atttcagata ggcaatcatg ttgaaataat caatgaaggt
     1981 gattgtattt attatgacag tatgaatccg cacggtatga tcgccataga aggtaaggac
     2041 tgtaagttcc ttgctatact catctgataa aataaggaac ggaaagagat ttaatggaac
     2101 aggttaaaaa ttactataag agatttgtta aagagggctt tgataagaac ggcattctca
     2161 ataagttcga gannnnnnnn nntgatatca tagataaatt cggcgagata gatcctgaaa
     2221 gaaaagcgct tattcatatc gatctgcagg agaaccgcca cgatttttct tattcacagc
     2281 tgtcaaagct gtcgaccagg gcagctaatc tttttgcttc atacggtatc aaaaagggcg
     2341 ataccgtaat gttggtgctt aaaagaaatt atcagttctg gatcgcaata atcggtctta
     2401 taaagcttgg cgctatagcg atcccggcaa cgcacttgct tacaacgcac gattttacgt
     2461 atcgctttga gcgcgcgagt gttaaggcaa tagtatgtac cgggtataac cccgatatcg
     2521 caaaatacgt tgacgaggct caggaagaga tacacgccga cgggcttatc aagtttatag
     2581 ctaacgggca caaggacggt tgggtgtcat ttgatgacga ggtcgcaaat atgcccgaaa
     2641 ctatggagcg tgtacaaacc gatacaagag agcatatgct gctttatttc acgtcgggta
     2701 caacgggcta tccgaagatg gttatccata atcacaggta cgcacttgct catatacaga
     2761 ctgcggcgca ttggcagaat gttgacccgg acggtattca tcttactatt tccgatacgg
     2821 gttggggaaa agccgcatgg ggcaagctgt tcggtcagat gtcgctcgga gcctgcgtat
     2881 tcgtatacga ctttgataag ttcattccga cagatatgct caggataatg cagaactaca
     2941 agatcacatc attttgtgcg ccgcctacta tgttccgttt ctttataaaa gagggacttg
     3001 aaaattatga cctttcaagt cttaagtatt cggctatcgc gggtgaggcg ctcaatccag
     3061 aggtatacaa caagtggctt gagtttaccg gtttaaagct gatggaagct ttcggtcaga
     3121 ctgagtctac cgctctgctt gcaaaccttg taggtatgac accgaagccg ggttcaatgg
     3181 gtaagcctac acctctttac gatgttgatg ttgttgatga tgacggaaat cccgttcctg
     3241 tcggagaggt tggagagata gttgtacgtg ccgagcgcgg taaagacgga ctttttgacg
     3301 gctattaccg tgatgaggag cttacagacc aggcatggca
//
LOCUS       DAWAOG010000203         3337 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3691_length_3337_cov_1.935405, whole genome shotgun sequence.
ACCESSION   DAWAOG010000203 DAWAOG010000000
VERSION     DAWAOG010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3691_length_3337_cov_1.935405"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1014 a          731 c          769 g          823 t
ORIGIN      
        1 acgcaacact ttacggcaag attgagctgt aagacttgca gaaattgacg ttttttcaat
       61 agattttgtg catttttgaa ttattgcaca agcaaacttt caaaatttgt gcaaaatgac
      121 ctattgacaa atcgcacgat atagtgtatt ataataactg taatcagaaa cgaggacgtt
      181 ttgagggatc aaagcgtcct tttcttattt ttcaaagaac atcttaacga aagtaggagg
      241 aaaaggaaaa tggcagaaaa aacttatgat gtaacggagc tgtttggctc ggacgtattc
      301 aacgaggcag taatgaaggc aagactgccc aaggcgatat tcaaagagct gatgaaggta
      361 atgcacggta atgcagagct tacacttccc gttgccgaag tagttgcaag cacaatgaag
      421 gactgggcag tagaaaaggg tgcaacacac tacacccact ggttccagcc tatgacaggt
      481 atcacagccg agaagcacga cagcttcatt tcgcctgtag gcgacggtac agttatcatg
      541 gagttctcag gcaaggagct tatcaaaggt gaacctgatg cttcatcgtt cccctcaggc
      601 ggtctgcgtg ctacattcga ggcaagaggc tacacggcgt gggatcccac atcatatgca
      661 tttatcaagg ataacacatt atatatcccc acagcattct tctcatactc aggcgaagcg
      721 cttgacaaaa agacacctct tttaaagtca atggatgctg tatcaaagca ggcattaaga
      781 atattaaagc tcttcggtaa cacaacagca aagagcgttg ttgctacagt cggcgcagaa
      841 caggaatact tcctcgttga caagaagaca tacgacaaga gaaaagacct tatcttcacc
      901 ggcagaacac ttttcggtgc agctcccgta aagggtcagg agcttgaaga tcactacttc
      961 ggcgttatca agcagagagt aagcgactat atgaaggagc tctacaagca catctgggcg
     1021 ctcggcgttt cttcaaagac aaagcataac gaggttgctc ccgcacagca cgaatgtgca
     1081 cctatctttg agtccgctaa ccttgcggcc gatcacaacc agctgatgat ggaaatgatg
     1141 aagaaggtcg cacttgaaca cgatatggta tgcttactgc acgaaaagcc tttcgcaggc
     1201 gttaacggtt caggtaagca cgataactgg gcactgtcaa cagatgacgg tcagaacctg
     1261 ctgaaccccg gtgattcacc tatggagaac gcacagttct taactttcct cgtagctatc
     1321 atcaaggctg ttgacgaata tgcagacctg ctcagactct cggtagcaac tgccggcaac
     1381 gaccacagac ttggtgctaa cgaagcacct cccgctatca tctcaatctt cctcggcgaa
     1441 gagctgaacg atgttatcaa tgcacttgtt gcaggtacaa atgttgaggc aaagcacgca
     1501 cagtttgata tcggcgtaac ctcactgcct aagttcccca aggatacaac agacagaaac
     1561 agaacgtcac cgttcgcatt cacaggtaac aagtttgagt tcagaagcgt tggttcttca
     1621 ctcaacattt caggtcctaa catagtactt aacacgatcg tagcagaaga gcttgaacag
     1681 ttcgctgatg agctcgaaaa ggctgacgac ttcaagaagg ctcttaagga actgctcgta
     1741 aagacaataa aggagcacag cagagtaatc ttcaacggca acggttacga tgccgcttgg
     1801 gtaaaggaag ctacagaggt aagaggtctg cctgagctta agtctacagt agacgcactt
     1861 ccccacttcc ttgacgagaa aaatatcaag gtatttacaa agcacaaggt attctcagaa
     1921 gttgagatgc actcaagaac agagatactg cttgaaaact acagtaagac gatcaacatt
     1981 gaggctctta caacagccga gatggctaca aaggaaattc tccccgcagt tatggcttac
     2041 gagaagcagg tttgcgatgt acttctgtcg aagaagaatg caggcgttgc atataacctt
     2101 gaacagtcta tcgctaacaa gatcgattct ctggctgaaa atctcaacgc taagattgca
     2161 gagcttgaca gcgtgatcgt aaaggctcag ggcatttccg atgcttacga agccgctaag
     2221 tgctaccacg atgaagtatt tgctacaatg cagagcctgc gtgctgttgc agatgagctt
     2281 gaaacgatcg tcggtgaaaa gtactggccc ttccccacat acgaagagct cctgttcaac
     2341 gtataattat agagatacgt ttatcaaatc ggcttagagg gatttttacc ctttaagata
     2401 atctgcaccc ttttcctatg gctatggcac tttggtgtca tagccgcttt tctttttgtg
     2461 aaatctttat ttataataac tatatttacc gatttttcgt catattttca cattatccgc
     2521 tttacaaatg cgccaaaatg gggtatactg taacagtcag gattatatgc tgtgaaagga
     2581 atttaaaaaa atgaccgaat acaacgacat actatacatt gtagtaccct gttataatga
     2641 agaagcagtg cttcacgaag catcgaaacg actgaaggaa aagatgactg ctctcataaa
     2701 cgataaaaaa atatccgaca aaagccgcgt tctgtttgta gatgacggca gtaaggacaa
     2761 gacatgggag ataatcgagg agctccacgg cagtgacgat ctgttctcag ggcttaagct
     2821 ctcccgtaac aagggacatc agaatgcact tcttgcaggt cttatgacgg caaaggagct
     2881 tgcggatata tccatatcaa tggacgcaga cctgcaggat gatataaacg ttgtcgatca
     2941 gtttgtagac aaatatcatg acggctgtga catagtatac ggtgtgcgtt cttcaagaaa
     3001 gaccgacacc gcattcaagc gcggaactgc cggtatgttc tacaagttta tgagattgct
     3061 cggagttgac attatcgaca accacgccga ttacagactt atttcaaagc gcgcacttga
     3121 agcgctgtcg cagttcaagg aggtcaatct gttcctccga ggtatagtaa agcagatagg
     3181 cttcaagagc gatatcgtat attacgagcg cagtgagcgc tttgcgggcg aatcaaagta
     3241 cccgctgaaa aagatgcttg cgtttgcgtt tgacggtatc acaagcttct cggtaaagcc
     3301 gataaggctt gttacggtag taggtttcct tgtattc
//
LOCUS       DAWAOG010000204         3336 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3692_length_3336_cov_2.693996, whole genome shotgun sequence.
ACCESSION   DAWAOG010000204 DAWAOG010000000
VERSION     DAWAOG010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3692_length_3336_cov_2.693996"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          672 a          817 c          763 g         1084 t
ORIGIN      
        1 cccgcaacgg cgaattgtca gcggcgcccc gccgctagta gttggagtcg aaggtttttc
       61 ctttgccgcc gagctttaca ctgctgtagt aatctatctt ctttttatcg accttgagcg
      121 actcaagctc tgcctttaca tcctcgtagt tctgacggac tactgcggta acctttttat
      181 ccttgacttc aatttcgctt ttaatatcgt gaaggtggcg catcttggat tgcaactgcc
      241 ttaattcata tgaaagacta tctgtaacgg gcttgttgtt cagtatattg cggataagct
      301 cgcgctcggc aggaacctgt gcgtttgcta catccattat agattgcttt acctgacgca
      361 ttttatccat aagctcatta cgggataaaa gcacctgctg aatgacttca aggtcgtttg
      421 tgaccatacg gtcagtacga cggtcatact cggtcataag ctccgtcagc tcgtccatga
      481 gcgtaagcag tgtttgtgca tctgcctttt tcattatatc ggattccttt ccggtggggt
      541 ttaaacaccg tgtattttat gcctgttcct gcatagcggg ctgtgccgac ttttcctgcg
      601 cttccatctg ttctttgctc atgctgttta tctccgtaaa agcttcacgc agttcggcta
      661 tcataggcag aagagcatct accttagcct tatccttttt cacgtttgct tcaacgatct
      721 cacggttgaa atattcatac agcgagtcaa gatttttaga tacgggtatt ttcatatcaa
      781 ggcaggaacg cagggcgttt atcacgtcct gcgcctttat aagcgccttg ttggtttctt
      841 cgtagttctt taagggaata tagtaggacg ctctgttgag ctgacgctca gcctcctgat
      901 acagccttac cactatctcg cctggggtga gagtagtgac cgattgcttt ttatatgcag
      961 aaaatgcgtt attcatatcg tattccttac gctgtgtatt tttacggggt catcagaact
     1021 gcatcagctg ctggatatag ctggtctggc tattgagcga acccatcatt gtttcgagat
     1081 ttgagaactg tttccagtat ctgtcctgct gcttttcata tcttgtttca agcgacgaga
     1141 taagcgtgga aatactcttc aactgatcat atatcgagtt atccgttgca gaagtgcctg
     1201 tctttgtgcc tgcaagctgt acaagtgtac ccttgtcttt tcttgcacct gttgtcttta
     1261 cagctcctgt aagcacacca tcaagcttgt gcatgatgcc cgtctttacg gtgttgccgt
     1321 tttcatcgac cgttgtgcct gcgaacaggt ctgctatctg gtcagcatac tgctcaaatg
     1381 cagcttcaag ctttgtttcg tccagctcaa gcttgccgtg gtcaccccag tcgtcgcttg
     1441 tagtaatacc catgctgaac aggctgaatg tctgtccgtc cgctgtcttt accgtagaat
     1501 aaagaacgga acgcatcttc tgcattactg tcgatactgt gctgtcgttg tagagcagac
     1561 cttccttagc cttttcttcc cacttctcct gctgtttttc ggataaatcc atatcctcaa
     1621 tatcgtcatc ggtaagtgcg tagtagtcgg agttcggctt ctggtcaagc tgaccgtaaa
     1681 cttcttcgat aatcttgttg taatcttcaa cgaatgactt tatagcgtct atcgcactgc
     1741 tgttgtcctt ctttacctca taagagaact ctgcgccctg tgcggcagat gtgaaggtca
     1801 ttgttgtgcc gtccgcagtg aagctgtttg acgcggtttc tatcgtttcg ccgtttactt
     1861 caagctgcag gttgcgtccc tgcgtcagcg tacccgatgt aagaccgagt gtgctgagaa
     1921 gctccgcgcc cgcgcttccg ttctctgcgg aaaattcgat ggatgcggct gtgccataac
     1981 ccgatgagct gagagcaaag ctgtttgtaa gtgtgctgaa cgtcattgtt gcgccggcgt
     2041 tcttgctggc gttaatttcg ctcatcatct ttgctactgt ataatccgat gtgaatgtga
     2101 acttagtgcc gtttatagtg aaggtcgcac tgtcctcaac acctaaatcc ttaagtgttg
     2161 tggctgttgt gacctgtgct gtagtctttc tggctgtaga tgcaccgaac gtattccgcg
     2221 ttgcgggatc tgcggcaacc gcgagctttt cgccgtttgc ggctgttacg gtgccgtttt
     2281 cgtctatgtt aagacccgta ccgtcggaca gcttaacctt tcctattgcc ttattcagct
     2341 catacttgtt tgcataagcg gcaaggtcct cgttggacag cttgaaatcg tctgcattaa
     2401 gcgtatcctt atatgcggaa agctcgtcat aatccttgtc gaactgcgct ttgaatgcgg
     2461 tttcgtcaaa gtcctttgcg tactgctcgt caaatgctgt cttgaaagcg ttctcgtcaa
     2521 attctgcctg agtcttcttc cagtcctcaa agctcgtgcc ttctgctata gagccgtctg
     2581 ctatgccgtt gtcatagttg gttttcagct ctttgtcata ttcggctatg gcttttgtct
     2641 tttcgctttc aaactttttg tcgtaatccg cctgctttgc tttgtcgtaa gcctgcgtcc
     2701 ttaaagcatc aaaatctaca gaaccgtcag ctttagccgc gttatacagc ttttcagcct
     2761 gctcgccgaa ataaaggttc tgatattcct ctcttttctg agcaccgagc gtagtgctgt
     2821 ctgtctgatt gaaataatcg gctgtgtagc tgtcaaaagc aacgcttact gtttcgtcac
     2881 cgacagttat attgaatttg tttgtgcctg cgttgatatt tccgcttaag tcaaatgtga
     2941 agttaccata catattggca acacctgtaa gcgacatcgc ctgcctgtcg gaggatatga
     3001 gcttaccgct ctcgttgaca taaaccagac cctgaccgct tgtgtttgat gcgccgtatg
     3061 cgtctttaag ctggctgttt atctgcgagc gtacactgtc ggcactgccg ccgttgaagt
     3121 ttataacagt tgacttcgtg cctaccgtta tattgaacga gtagttctgt gaagcatcat
     3181 tgtactgtga taagtcaagc gagcttgcgt ccattgcact gccctgatac ttagcctgtg
     3241 tggcggtctg gagcaccttt acattgtatg taccgggtac tgcatttgtg cctgagcgta
     3301 ccgatacacc ggcaggagta ccctctttga gtatgc
//
LOCUS       DAWAOG010000205         3324 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3712_length_3324_cov_2.098501, whole genome shotgun sequence.
ACCESSION   DAWAOG010000205 DAWAOG010000000
VERSION     DAWAOG010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3712_length_3324_cov_2.098501"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2099..2108
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          832 a          756 c          761 g          965 t
ORIGIN      
        1 ctttgcggca gctattacct cttcatcggt cgcgtccttt ctgccaatgc gtatattttc
       61 aaggatcgta ttgttgaaga gggtcacgtc ctggaatacg atagaataga gcgaaagcag
      121 tgtttcgggg tcgatctgcg atacgtccat gccgccgact gtgaccttgc cgctgtcggc
      181 atcccagaaa cgtgcggcga gtcttgaaac ggtggtcttg ccgccgccgg acggtccgac
      241 cagtgcggtg acttcaccct gctttgcggt aaagcttacg ttatgcagca cactttcacc
      301 gtctttatat gagaagctta catcgtcaaa gcgtatgtca taaccgttgt ttgtaagctt
      361 gtttgcgcct gtttgttcat cgtgcaggag aatttcgttc attcgcgcta tattggtatc
      421 ggcacttatc actgcggcaa aattctgcct gtaacggttc ataaagacgt gacgctacaa
      481 gtaggaacat aaagaacgtc agcagatcga tcttgccgga tgttatcagt atgccgcctg
      541 taagagcgac agtgcctatg ccgagcttaa gtacagtgaa tgacgaggta acaaaagacg
      601 ctgtggtaaa ttcagccgtt gtagtgcagt gttcgacatt ctttatcttt tcttttacgc
      661 tttcaaggta tttttcttcc gcattgttcg ccttaaggtc tgtgatggtt tcgatacatt
      721 cctgtatgcc gtctgcacag tcaagctttg ctttcatatt cttgcgggca acctttttct
      781 gtatcttctt tgacaagatc ataatgataa atgaaaccgg aacgacccag aatgccgcaa
      841 gagccattcg ccagtcaaac ataaacagtg aaagcgctat aagtacagtt gaaattatcg
      901 agccgacaag ctccgggata aaatgtgaaa acgctgtttc aagaaaggcg caatcagcca
      961 taaacgccga gctcagatcg gcaaggtcgc gctttgcaaa gaaggagaga ggaagctttc
     1021 tgagcttttc cgcaagtgat attcttctta ctccgctttc tgtatatgtt gcgaaaaaag
     1081 tcgcgttata ctgcagcgtt gccgcgaatg cgatgaatgc gaggcaaagg actattcctg
     1141 ttatatagaa ccatattctt tctcccgtga tcgtgcctgc cataagatcg ctcaccataa
     1201 aataaagcaa tgcgacaggc ggcataaacg acaggttctg taatacgcag gcaagacagc
     1261 cttttataag gtcttttgca ccttgtcttg aaagtgcgta ttttctcata agcttctctg
     1321 tcatatattt gcctccttta ctttccatac agccgaggtt tcgtactcgc tcagcatatc
     1381 ggcgaacatt ccgccgcttg cggtaagctc cgcggcagtg ccgctttcgg tgatttttcc
     1441 gtcacaaagc acaaatatcc tgtctgcacc aagtactgtg gtcagtctgt gagctatcat
     1501 tattacggtt ctgccttcgg acagcttgct catagcctgt tgcaccctga cttcgttatc
     1561 ggggtcggca aatgccgttg cttcgtccat tattacaata ggggcgtttt tcagcattac
     1621 tcttgctatg gctattcgct gctgttcacc gcccgaaaga tatatgccgg ctgtgcctat
     1681 gaccgtgttt tctctatcgg ggagtttttc tattatatcc atacactgcg cgtttgttag
     1741 tgccgccatg acttccttgc gggtagcggt gggtctgccc ataagtacgt tttcgtagat
     1801 agttcccttt ataagtctgc tgttctggaa tacaaaggaa acagtgttca taagaacatc
     1861 tttcgggata tcctttacgt tttttcctcc gacaatcacg tcacccttat ctgtatcaaa
     1921 gaagcgtaca acaagctgag cgagtgtagt cttgccgctg cccgacggac cgacaaaagc
     1981 gactgtctgt cccggctcga ccctgagaga tacatcgtca attacatttt ggcttttgtc
     2041 gtatgagaac gtgacattct taagctctac cgagctgtcg gcaggagaaa cgcctgtgnn
     2101 nnnnnnnngg atagtatcga tcctgcctaa agcgtccgtt actatcatct cattttcact
     2161 tgacatcatt attttggtaa gagtggtagc gattatcggg gtaaagataa tgtaaaatat
     2221 cagattgagc aggaatgtgc cgtcaacgac gccttgcgta aatatgatgc cggcaaggat
     2281 aagaagcgcg aatattccgt ttatgacggt gatgtagact atcataggta tgcgtaattg
     2341 tttggtatat gatattaccc attcgccgta gcgatcgata gaagttttaa acttcttgaa
     2401 tgaaaatacg gtctgtccga atgtctttac gaccgggata cctcttatgt actctaccgc
     2461 ctcgtttgac atatcgtcaa gtgcgttctg atactctttc atttttcttt gcatttcttt
     2521 tcccgtcatg ctcatcatta taaagaatga aaggacaacc ggaataaggc tgataagacc
     2581 gagccgccag tcgaagatga acagaaagac aagcattacc gcaggagttg ctattgagcc
     2641 cgccttgtct ggtaactggt gcgcaaggta ggtttctgta gcggcggggg cttcgtctat
     2701 tgtttttctg agcttaccgc ttcctgcttt ttcaatgaca cctataggca gttttgttat
     2761 atgggtgatc gcatcgatgc gcaaattggc agcaattctg aatgcgccga gatgagaaca
     2821 cataagagcg catatgtaaa caagcattga gccgatagcc cagataacag ccgccatgcc
     2881 gttagcggca atgtcggtcg ccttggaaaa gtcggggttt acctcgatca gttgttttac
     2941 tatcagccac agatgaataa acggcataag agccattgcc gcacttacag cagacagtat
     3001 ccacgaaaga tacaggagta ttttccgctt gcccgcataa gacagcagtc gtgaaaggtt
     3061 tgattgcttt ttcaaaatga tccctccgga aaaataaagt atgttaggtt taactaactg
     3121 ttcatcgtaa ttaaacgcat aaaagcaaag ctcttatgcg tattacttat taaatcaggt
     3181 ctgtcccatg attttcatcc agcctgctgt gtagaagtcg cgcatttccg ccagataggt
     3241 ttcagcttcg ttataaggga tatcatgtat aacaagctca aaaaacgtat tgatcatacc
     3301 ggtcgctatg atatgctcta ggtg
//
LOCUS       DAWAOG010000206         3269 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3783_length_3269_cov_1.975420, whole genome shotgun sequence.
ACCESSION   DAWAOG010000206 DAWAOG010000000
VERSION     DAWAOG010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3783_length_3269_cov_1.975420"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    812..839
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2148..2157
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          906 a          649 c          907 g          769 t
ORIGIN      
        1 ccgttccttg cggtagaggg ctgcggtgaa aacgccgcaa tcaagctcaa ggaagttatc
       61 gacaagggcg atttcatctg tcttgaggat attcaggcac agtcgggtat aaacagcacc
      121 gttctcggca agctgtatga tatgaatgtg ttcggggatc tccctcaatc cgcacagata
      181 tccttcttct aagcgggcgg cgagtcgccg cagacaattc cgcctttgga gcagttacag
      241 ctatccgcag gatagcaaaa cggcgggcgg caatgttcgt tccgaattct gaccttttta
      301 aaggtcggtg atgggctgaa gtacggcgga cggtcgtgga accggtgata cagcagacct
      361 taaacaacaa atattgattg ttgaaaatat aaagtgtttt aagaaaggca aggtaaaaaa
      421 ccaagtgaac tattcattca atgtgagaat tctcagtcat ttttatcaca gcgcagtaaa
      481 ggctgaactt gaaagacgga attttccgaa gggtatggcg aagaagattt ttaccgagca
      541 taaggcggta gttgcccgtg caaaagacat cggtaagtca aagctgatga gctcttatat
      601 gatgggcgca tattttatcg ctatgaatcg cagtacagga aagacggcgg aagaaaatta
      661 cgaaatttta aaggacggat tatgcgcttc gaagctgttt cacaaagctg taggtaatgt
      721 agacagttat cttgacgaaa agaagatgcc cggacgtctt gcgtggtcgg aagaaagcca
      781 caagagaaaa tatgagaacg actgggtagt tnnnnnnnnn nnnnnnnnnn nnnnnnnnng
      841 gcgctgatat gtgcgacttc agatacagca gaaagtaaat ttgacttaaa aggataacga
      901 tacacaatga aaacagcggc aattatatgc gaatacaatc catttcataa cggacataaa
      961 tatcacatag agcaaacccg tttacagcac ggcgcaacgc atattgtatg tgtaatgagc
     1021 ggaaatttca ctcagagagg cgatgttgcg cttgcggaca aatacgagcg tgccagagcc
     1081 gctttgatgg gcggggcaga tctggtggtc gaactgccta ctccgtttgc actttcaagt
     1141 gcggagcatt ttgcaatggg ggcgtgccgt attgccgatg cgctcggctg tgtcgatatg
     1201 ctgagcttcg gcagtgaatg cggcaatgta tctgtcttgg aagaggcggc tggcgctgtt
     1261 gattatgcgg tgcagacgga agagttcttc agcctgatgc gtaaaggcag ttcatatcct
     1321 gcggcgctga agcaggtcgt ggagctaagc tacacgccgg atgtcgtggc aacgcttaca
     1381 gagcctaaca acacgcttgc ggtcgaatat atccgcgcgc ttgataagct gggcggtatg
     1441 ataaagccgg tcacggtaat gaggagcggt gcggcgcacg acagtgaggt aggcagtgac
     1501 actgtgataa gcgcgtcaaa gctcaggaag atgctcggtg cgggcgaaga tgtaagcgcc
     1561 tataccgatt atgcagaata tgggaatttt gcacatatcg agaatattga aacggcgatc
     1621 ctcgcaaagc tccgtactat gtcaaagtcg gagtttgagc ggcttccgaa cggtacgggc
     1681 ggtatggaca gcagaatata caaggcggta cgaaccgcgg tatcattgcc gcagttactg
     1741 cttatgataa agtcaaagaa tttcactatg gcgagaatac gcaggcttgt actgtgtgca
     1801 ttcctcggca taacgggaaa cgaccttaaa gatccgcccg cgtatgtcag aatactcgga
     1861 atgaatagcc gcggcaagga aatacttgcg gcgggagaac ataaactgcc tgtagacacc
     1921 tcgctgtctg cacttgcaaa aacaagccgt gaggcggagc attttgcaag gcttgaagaa
     1981 agggcgggaa acatatatgc gctggcgttg gacaatagac gaccgtgcgg tatggaatat
     2041 acttcaaaac cgataatatt gtaaacggag gttaagttat ggataactcg gggaaataca
     2101 gagcgctcag agaaaagcac aaggaattta tctatcatgg ctatgacnnn nnnnnnnggc
     2161 tatgacattg ccgacgatgg aatattcttt catttttcga tagatgatta tcatttttac
     2221 ccttcgtgga agacggagag cggactgctc aaaaacgtaa cgccgtttct tgaatatgta
     2281 atattcaacc tcggtatggc ggagcttgta agctactgga agtgtgcctg tccgcttgta
     2341 gtaagggtgg agtgcggttc gcttgaagaa aagcagtgcc tgtggtggaa aaagctctat
     2401 ttcaacggac tgggagagtt tttctacaga aacaatatag acgctgattt tgacagcttt
     2461 atgcagatag tgccggatga taacagcagg cacaaataca gctgtgaaat ggaagtcggc
     2521 ggttatctcg tggcggtcgg cggcggcaag gacagtgtgg tatcgctcga actgctcaga
     2581 aaatatcacg atgaaacact gtgctatata ataaatccga gaggtgctac ggtagagtgt
     2641 gccaaggtcg caggctttga cgaaagcggt attgtaggac ctcgcagaac gatagacagg
     2701 gcactgcttg aacgcaatgc ggacggatac ctgaacggcc atacgccgtt ctcggcggtg
     2761 gtcgcgttct cggcttatct gtttgcatat cttcacggca ggaaatatat agtgctgtca
     2821 aatgaaagca gtgcgaatga gacctatgtc agcggcaggc aggtaaatca ccagtacagc
     2881 aagagcaccg agtttgagcg tgatttcaga agctatgtta cggattatct tgatgacagc
     2941 atacagtatt tcagccttct gcgcccgtgg agcgagtggc agatagcaaa gaaattcgtt
     3001 acctatccgc agtatttccc cgttttccag agctgtaatt taggctcaaa gactaatgta
     3061 tggtgtgcgg actgcgcaaa gtgcctgtat gtctatatat tgctgtcggc gttccttgac
     3121 gatgaaacgc tggtgaagat attcggaaag aatatgctcg actgcgaaaa gtacgaggat
     3181 atgtttgacg gactggtgct tgacggcaag gataagccgt ttgagtgcgt cggcacaaag
     3241 agcgaggtaa ggttgtcgct gtatatggc
//
LOCUS       DAWAOG010000207         3253 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3807_length_3253_cov_1.897436, whole genome shotgun sequence.
ACCESSION   DAWAOG010000207 DAWAOG010000000
VERSION     DAWAOG010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3807_length_3253_cov_1.897436"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          993 a          690 c          772 g          798 t
ORIGIN      
        1 gtttcggctt cagatgcgga tttttaggac ttcttcatat ggagataatc caggaaagac
       61 ttgaacgtga atataatctt gaccttgtta caactgcccc gtcagtagta tacaagataa
      121 caaagacaga cggcgaaacg atttacattg acaacccgac aaattacccc gatccgtcag
      181 aaatagaagt tgccgaagag cctatggtaa aagcaaatat ctacacaccg tcagaatatg
      241 tcggaagtat aatggatctg tgtcaggaaa gaagaggcac cttcaaggat atgaaatacc
      301 ttgatcttaa ccgtgttgag atgcattatg atcttccgct gaacgaaata gtatacgact
      361 tttttgatgc gcttaagtca cgcacaaagg gttatgcaag ctatgactat gagatgtcgg
      421 gatttgttcc gtcacgactg gtaaagctcg acatactcct taacggcgaa gttgtcgatg
      481 cgctctcgtt cgttgttcat gcggataagg cttatacaag ggcaagaaag atcgcagaaa
      541 agttaaaaga caacattccc cgtcagctgt ttgaagtgcc tatccaggcg gcagtcggcg
      601 gtaaaatcat tgcaagagaa accgtaaagg caatgcgtaa ggacgtgctc gcaaagtgct
      661 acggcggcga tattacccgt aagaagaagc tgcttgaaaa gcaaaaagag ggtaagaaac
      721 gtatgcgcca gcttggcagc gtcgaagttc cgcaggaagc gtttatggca gtgctcaagc
      781 ttgatgaata aaggacttta tatccatata ccgttctgcc tgtcgaaatg tccgtattgc
      841 gacttctact cagtggcatt tgacaacgat acggcaaagc aatacaagga cgctgttatt
      901 agaaatctgc ggtattacct gttgcaaaac agcttcctca gatttgacac ggtatatttc
      961 ggcggcggta ctccgatact gctatggcgt gagatctgtg atatcatgga cgaaatacag
     1021 ccgtatatga ccgaaaatgc cgaggtcact attgaagcga acccgtgttg taccgatgaa
     1081 agctcactgc attcattaaa agcgcataac gtgaacagaa tttcattcgg cttacagagc
     1141 ggaaatgata acgaactgaa agcactcggc agacggcata acagtaaagt cggtgcagat
     1201 gcgataaata tggcgtacaa agcgggattt gagaatattt cgggcgatat aatgctcgga
     1261 gtgcctcttc agacaaagat aagccttata gaaactatcg gctatatgac ctccctgccg
     1321 ctaagtcaca tttcggcata tctgctgaaa attgagccgg gcacgccgta tgctaaaaaa
     1381 cagcttacac tccccgatga cgatggatac gccgatatct atcttgaaac ggtaaaccgt
     1441 cttgcagaca aaggatataa gcaatacgag ataagcaatt tttcaaagcc gggcatgata
     1501 agcaggcaca atctgaaata ctggcgatgc gaagaatatc tcggtatagg acccgccgca
     1561 cattcttatt ttaacggaga acgttttgct gtcgggcgtg atttgggagc gtttatccaa
     1621 agtgaggttc agcctgccgt tatcaccgag cgtgaatgcg gcgactatga agagtgggca
     1681 atgctgaagc tcagactcag tgaaggtctt gatttcaaaa ctgccgaaga acgttttggc
     1741 gtatcaaaag aggatatttt aaaaaagtgc agacttatcc cggataaact gtacagagcg
     1801 gacgataagg ctatacacct tacgcccgag ggatttctgg tatcaaatgc cgttatcggc
     1861 atattaacag atatatgatt tttggaggaa ctatgaacaa ggaattactt aaagccgata
     1921 tcgaatcggt actgttcgac aagtatgaag ttacagctga caaggcacag ccctatcagc
     1981 tccacgaagc actttcatct gcaataatga agcagatcgc acccgtatgg aaagacagca
     2041 ggaaggctca tcttgccaca cgcagagctt gctatttctc tgccgaattt ttagtgggca
     2101 gagccattta caacaacctg ctctgtcttg gcattgaaga cgatgcaaga gatgcccttt
     2161 cttccctcgg cgcaaagctg tccgaccttg aagagattga agatgccgca ctcggaaacg
     2221 gcggtcttgg cagacttgcg gcttgctttt tagacagtgc cgctaccctg tcattacctc
     2281 ttgacggcta cggcatacgc tataagtacg gtcttttcaa gcagtctatc gttgacggct
     2341 tccagaagga agaggctgac gactggacaa agtacggtga tccgtggagc agaagaaacg
     2401 aaaacgacaa ggtcacaata agctttgcag atcagactgt tgtagctgtg ccttacgata
     2461 tgccgattat cggatatggc actaagaaca taggcactct gcgtctgtgg caggcagaac
     2521 ccgtcaatac gtttgatttc cttaagttca atgcacagga ctatgtaaac gcaagcatcg
     2581 agcagataaa ggctgaggat atatcgcgag ttctctaccc caatgatgat acaagagagg
     2641 gtaagatact ccgcttcaag cagcagtatt tcttcagcgc ggcttctctt gccgatatcg
     2701 taaagaagca taaatcatca ggttacgaca tcgaaaaact gtacgaaaag gttacaattc
     2761 agcttaacga tactcaccct gttatatcta tccctgagct tataagaata cttgtagata
     2821 acgagggact ttcatttgaa aaggctcttg aaatcgccaa aaagacattc aactatacaa
     2881 accacactgt aatggcagag gctatggaga agtggggtat tgatatagta aaggaagttc
     2941 tgcccagaat ttacgagatt attttacaga taaacgaagc cttcgttgct gagatgtatg
     3001 caaacgatat gcctaagggt aagaccgact atatgaagat gatctcaggc agcgttgtac
     3061 atatggctaa gatggcagtt tactgctcaa gctatacaaa cggtgttgcg gcgctacata
     3121 ccgagatatt gaagaacgat gttttaaagg actggtatct gctctttccc gaaaagttcc
     3181 agaacaagac aaacggtatc acaccaagac gctggattgc gctttgcaac aaggagttat
     3241 catcgctcat aac
//
LOCUS       DAWAOG010000208         3237 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3836_length_3237_cov_2.062539, whole genome shotgun sequence.
ACCESSION   DAWAOG010000208 DAWAOG010000000
VERSION     DAWAOG010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3836_length_3237_cov_2.062539"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1208..1217
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          831 a          783 c          720 g          893 t
ORIGIN      
        1 aaaggaaaat aaaatgggaa aagcattgat aataggagcc ggcggcgttg ccggagtagt
       61 agttcataag tgctgtcaga acagcgatgt atttaccgag atctgcattg cgagccgtac
      121 aaagtcaaag tgcgacgcgc tgaaaaagca ggtcgaggaa aagggcacaa agacaaaggt
      181 aacaactgca caggttgacg cagacaatgt tcccgaactt gttacactta taaacaagga
      241 aaagcccgat attgtaataa atgttgcgct tccttatcag gatcttacaa taatggacgc
      301 ttgtcttgaa acaaagacag actatgtcga tacggcaaac tacgagcacc ccgatacagc
      361 caagtttgaa tacaagtatc agtgggcgta tagggaaaag tttgaaaagg cgggcatcac
      421 agcgcttctc ggaagcggat ttgaccccgg tgtaacgggc gtgttctgcg catatgcaca
      481 gaagcattac ttcgatgaga taaactatat cgatatcctc gactgcaacg gcggcgatca
      541 cggttatccg ttcgctacta acttcaaccc cgagataaat atccgtgagg tatctgcaaa
      601 gggcagttat atcgaggacg gcaagtgggt agaaaccgaa cctatggaga taaagagaga
      661 gtacaatttt gagcaggtcg gcaagaagga tatgtacctt ctgcaccacg aggagcttga
      721 atcacttgcg ctcaacatca agggtatcaa gcgtatccgt ttctttatga cgttcggtca
      781 gagctatctt actcatctta agtgccttga aaatgtcggt atgacatcta tcaagcccat
      841 tatgtacgag ggcagagaga tagtaccgct ccagtttctt aaggcagttt taccagaccc
      901 cgcatcactc ggtccgagaa cagtcggaaa gacaaatatc ggctgtatct gcataggtaa
      961 aaaggacggc aaggaagtgc actacaaggt ctacaactgc tgcgatcatc aggaatgcta
     1021 caaggaggtc ggctcgcagg ctatcagcta cacgaccggc gttcccgcta tgataggtgc
     1081 aatgatggta atgacaggca agtggaagaa acccggcgtc tacaacattg aggaatttga
     1141 tcccgatccg tttatggacg cactcaataa gtggggcctg ccctggaagg aaagcttctc
     1201 ccctgagnnn nnnnnnnctt atcaagcacc gtagtttgcg gataggaaac taccttgttt
     1261 tcaccgccgt caaagtcaaa cgcctgaatt gccgaatttc ctgccgatac cgtaaacagt
     1321 acggagtgtc ccttgtcaag tgccaccgtc agcagtccgc ttgccgtttc cgaccagccc
     1381 gccgtcttgc actttaccat aactgcccca ccatatccgg aggtaacttt tccgtctgta
     1441 gcacccgaaa tatcaatcgt ttcaccgaat attccggttc ttatgtctaa gctgcagtca
     1501 acgatcgaaa ggtcattgct gtatatgccg gttgacgatt ctatgttcgt tcccgcaaga
     1561 cctgttgtta ttatactgca tctgtcgaag cggattgttt cagcgtgtac gccgtaatat
     1621 tcggctgccg cagtaaatgt catattgctg aacttaacac tgctcacact ctgtatagcg
     1681 taggtgtttc cggttactgt gaaattcggg ctgtcaccgc cgacaaattc catgctcatc
     1741 ggtgttgcat cggtgtagtc gaggtgtgac tgatatattg ctaacaagtt ggcagtagtt
     1801 gccgtgttac gtttagaaag cgtaacattt ccgtttatgt tcaccttaag gctgttcgag
     1861 ctgacaaaca tcagatatgt gtcagctgaa ccctgttcgt cacctatact tccgctgttg
     1921 ccaaaatcgt cgggctttgc gccgttcata tctcctgccg taagcgtaag attttcaaac
     1981 gtaatatccg aaacgctttg cttaatatac agcattatat tctttccgac acccgacagc
     2041 ttaagaccgt ttccggtcac tgtcagtata ccggtagctg tatcatactc aaagccgctt
     2101 gccttgttgc tgatgaacgt cttgctttcg gtatctataa cggagcttgc actgccgtca
     2161 gaaacggtca gatatccgct gagatccttt tcggctgtga atgctacatt ctttgacggc
     2221 attgtgaatt tgcttgcaac tgtgacatct tctgttgtcc aatcaagata gccgtctgat
     2281 ttttccttta cggtgacctc tgaaccaatc tttgcctttt ctcttgagct ttcaacaccg
     2341 tcaagcgagt agattacata aacgggagtg atagtacccg tcagcgtcat atcggacttg
     2401 atttcttttc cgatgactga atatccgttt tcgccaagcc atccgccgaa cagacagccg
     2461 tctgtatcag gagatacctc aagcatatca gccgtgaaca ccgttccgta gtcaaattcg
     2521 gctgttttat agctttcgta aaccttgttg gtatcaagat ttacaagacg gtaggttatc
     2581 gtcatctttt tcagcttgta tgttgctttg atctcggtct ttataatacc gtcgtcatta
     2641 ccgaacgttg tcggtacagc tttatcccag tcagcaaact catagccctc aggagctgtc
     2701 ggtacgggaa tgtcggactg cgaaacagta tcgccgtatt tgcctgttat ttcataacgt
     2761 accgtaccgt catcgaactc accgctcata gcatagaaca ctatgctgta gattttgtcg
     2821 gtagctttcc atactgcctt cagcgtcata tcgcccgtta ctctgaacga ttcacccggc
     2881 tgtacatcaa cattgccgtc accgtcaaga tcatagccgg aaagctcata atcgttgaac
     2941 ttatcatcct ttaaagcacc tgccgttgca cgatacagat agccgtactt tacctttacc
     3001 gtttcgttct ccgtgccgtt gtcatagtca aacgttacat cgaactcctt cggcgttctg
     3061 ttccacctta caattatagt cttgtaggtc ttgccatcaa tcgttgtgtt ttcttcgttt
     3121 atcggctctt ccttaagagt atatgtgtaa tactcgtcat catactgtgc aagcgcctca
     3181 acttcggcat catacttttt acaaagctcg tcatattcgg gtctgtagcc ctcaaac
//
LOCUS       DAWAOG010000209         3229 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3847_length_3229_cov_2.165406, whole genome shotgun sequence.
ACCESSION   DAWAOG010000209 DAWAOG010000000
VERSION     DAWAOG010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3847_length_3229_cov_2.165406"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    374..383
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2514..2523
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          748 a          745 c          661 g         1055 t
ORIGIN      
        1 tcgtttgctt cttcggtagg cttgtattcg tcgctgctta tataatcctg caaataatca
       61 cggtattcgc cgagagcttc aaatttttgc agggtaagct ctcccttgta ctcctcatac
      121 atttttctgc cgagtgacga tttatcctct acgcctattg caatacggct gtcgccgaga
      181 aatctcgctg tttcgtgctg tttgaacaga tttaccccga taagcgcaag cagaagtata
      241 aagaccgata agcgaagcac attctttttc agttcatatt taaacagctt catatcacat
      301 atccccgaaa taataaatac acacatcttc aagagtaggc tgtacagtct ttgcaccctg
      361 ctcgggaaga ttgnnnnnnn nnngtccgac actatgcgta aagtgtattt actgccgtca
      421 gataccgcat ttgatacacg catacgactc atatattcca tcaccttgtc actctcggcg
      481 gttatctccc acaccttgcc cgaaatatcg gaacataact cttcctgcgt gcctgttttg
      541 attattttgc cgccgttcat aagaacgacc gtcttcgcaa cataagctat atcggtgacg
      601 atatgcgttg caagtataac tatcttatct gcggcaaggg agcttataac atttctgaaa
      661 cgaatacgct cctttgcgtc aaggcctgcg gtcggctcgt caaaaatcag caccttaggc
      721 tcgcctacta tcgcctgcgc tataccgagc ctttgcttca ttccgcccga aaacgcgccg
      781 atttttttat ctgcgtccga gtgcagattt acacggtcaa gcacagaaag ggcatactcg
      841 tccgcattgt cgggagagta ttttttcagc gccatgatgt actttatgta gtcctttgct
      901 gtgaagttgc ggtaaaagtc catgccctgc ggcataaagc cgagatgtga caaaaagctg
      961 ttgtccccgt cgtcggaaaa cgctatgctt ccgctgtctt tgccgattat ccccgtgatg
     1021 atatttataa gcgtggattt acctgcgccg ttgggaccga gcagtccgta cactccttcg
     1081 gtgaaggtga gcgtgaagtc ctcaagtgcg tttttgcgct ttttgccttt cgtgtaggat
     1141 tttgtgatat gttcaagtgt taactgcata gcttattccc tttcgttatc tgtattttat
     1201 tctgtagtcg ccgtttgttg tggatagaat cacatattcg tttccgacga gcagagttcc
     1261 gcctactttc agtttgctgt cgcttatctc ttcttcaaag aacagctcac ctgtttttgt
     1321 atcataaatc cttaaatatg tgcctgtcgg ggttttgccg tcagccgcac ttatctcggt
     1381 cacagaggta acaatataag caccgtcggg agagagtgcc gagcttgtac ttccctcggt
     1441 cttatattcg gtctttatct cacggaattc ttttttggtc ttatcaaaga taacaaactt
     1501 tccgcttgtg tttttatgat cagggaagaa gatataatct ccctcgctgc tgattattcc
     1561 gccgccgtca tcggtaagcc cgtctccgtc atatattacg gtattgccgt cgctgagatt
     1621 tataaccacg gctgactcgg tagtgcttcc ctctttacac cgcctgaaaa ataccagctc
     1681 gccgtctgtg gcaagagagt aatcatagta attgtccggg aatgtgtaaa tcggattttt
     1741 gttttcgccg ttataatcac atacgcatag cgttgtactg ccgtttatat cctcatgtat
     1801 cagcttttgc gagtcgttaa gtataatacc gtcttcaatg ataaattctc cgcttttttg
     1861 cataggtact tcgcttacag ccgaaaaatc actatccagc agataatacg ctccgctctt
     1921 tacttcatct ccctcatatt catatttctt aataaccatc ttagtgccgt caatggcgag
     1981 agagtatgtc atatagcctt gcttttccaa ttcttcaata cgtactattt cacttacacg
     2041 cttatcctcg tctatgtcgg aaatgtacag ggctttgtta ttctcaataa ctgcgagcct
     2101 tttcccgtcc tgttcgatat atccgatgag cttaattgta aatgatgtat ttttcacaac
     2161 agcgccgttg tttattattt cctgcggagt gctgtcggaa gtatctgagc tgttatctgt
     2221 accggcaggt ctttccggct cttttacttc aaaatcgctg tttacaacaa gcttgggctg
     2281 tgtcatatca aatgaagcat actcttcaaa attctcgccc gtgtttttat gtattgcggc
     2341 atatataaag tcacctcgag caatattgtc aagctgtacg gaatatgtgt acagatatcc
     2401 gtctacaagc tctgcttcga tatagtcatt gccgtttata gttattagtt cgttgttttt
     2461 gtatagtgaa actctgtatt taccgctgtt ttcattttca taaagcatca gatnnnnnnn
     2521 nnnattgaga tttaatgtac cgtcctcgct gagccgtaaa acgcccgtgt cggtctttgt
     2581 gttctgatac atatttactc ttgttcgtcc gcttgtcttc aatggagtac ctgtgttatc
     2641 ggcgtacttt tccaaaaggg tttttgtgat aagctctttg cttatatcgg aagcgtagct
     2701 gtaatctgtg tatttaggcg taccgtcaag gcttatcgtg gtataaaggc aatgattggc
     2761 atggtgagtc ataccaaagg tggggtagct ttcatcgggt atatatgcgg cgttgaagtg
     2821 agcgaaaaaa gtaatcggca gctgcttttt acccttatcc tcactgctga taacagggtc
     2881 aaaagttatc tcccgctcga ttgtttcacc cggagcgata ttctcgaaaa tgagcatatt
     2941 gctttcccgc ataccacctg tactcagcgt ctgtacagtg ccgtttatca cggcagatat
     3001 gccgagtgac atctcatatt catttgtatc atcggcagta acagcaacag gaacggttat
     3061 tttttctccc gtataaacta tcggttttcc ttcattaacg gtacactcat aaccgcctgt
     3121 tgagtatttt gacagattct gagccgccgt gttttcttta atatcgtcgc cgataagggt
     3181 gtttgattca aatgtttcaa acgggtcggg tatatagctg tcgtatgac
//
LOCUS       DAWAOG010000210         3218 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3867_length_3218_cov_2.314891, whole genome shotgun sequence.
ACCESSION   DAWAOG010000210 DAWAOG010000000
VERSION     DAWAOG010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3867_length_3218_cov_2.314891"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          784 a          785 c          621 g         1028 t
ORIGIN      
        1 cggttagcaa gcttgtgctg tgaaacagtt atctcaaggt tcttttcaag ataatactgc
       61 aacgtttcat caattgtaag cacaagactg ttgccgtcct ttgccgcaaa gcgtgacgaa
      121 taatcatacg gcattacatc gccgtccgca tcagtggtag ataccactct gccgtcaacg
      181 ccctttaagt acgaattgta ataagcttca agtccgtatc cctcttcgtt ctcgtttata
      241 aatccgagaa cacttgccgc aagcgtaccg ttggggtagc tcctctcgct gttttcgaca
      301 gaataaaccg atacattctt gaggtcgttt ttcagaataa acttatttat cttctcgacc
      361 gtatcgcgat cgaccttttt ctttgccaca tagtaacggc tctcggtatc ctcgcacgcc
      421 ttgatcagtg tttcatactt tacgtcaagt atttcgctga gtcctttaca tatgaactcg
      481 cggttctgcg gctcatactc ttcaatatcg ccgggagata ttattatatc ccacaccgtt
      541 gtgctctgcg ccagcacctt gttgttgcgg tcgtatatcg ttccgcgttt tgccttgata
      601 gtaaagctgc tctgttgctg atcgtttgcc atcgccgaca tttctttact gttgactact
      661 gcggcattga acagagttat acttacatag cctgtaagga aaacgcacag cgctactatt
      721 acgaaaatta tgcgtattct catactccgt gtggtcttta cgttcatcgg ctgctccttt
      781 gctgtaagta atgttttcaa aaataacttt cgataaaaaa gcattatcct ttcctcgtaa
      841 acacagccgt atcaacgtgg aaaggagccc tgcttgttca tttatatgtc ctaaaatccg
      901 agatattcta atataccgtt aaaccagttc tggatatcaa caaatatatt gtcactgttt
      961 ttctcagcgc tttctgtcat agtacctgta ttcatctcga catatctctc ctgagatgcg
     1021 tttatcttct gcataccgag cttgctgacc gcatactgct ctacattatc aagcgacacc
     1081 gagccgctga gttgattatt aagcgcagtg ttgatgttct gtgcactctg caaagcctga
     1141 gtttcattgc ttatcctgag gttaacttcg ctgagtgtag ccttgctgta aaggaacatt
     1201 ataaacacag tgagcgtaac tgccgcaatc actaccagtg caacgggctt gcccgccttt
     1261 gcaacggacg atgtcgctac tttcagttcc ggctttttct cgggagtatt atgttctttt
     1321 cttttagcag atgtatcaaa cagtgacaag tcataagccg cgcttgttct gttgtgtggt
     1381 gttgtcatta tgttatcccc tttttatagt ttttctatga ctctgagttt tgcacttctt
     1441 gaacggttgt tcatttcaag ctccccgttt cccgctgtca cgggttttct gtatacaagc
     1501 ttgcctctcg gcttttttcc gcacacgcat acaggaaaat ccggcggaca ggtgcaaccg
     1561 gtgcaataat ctctgaacgt gttttttact atcctgtctt caatagagtg gaacgtgatg
     1621 atcgccagtc ttccgcctgc cgaaagcctt tcaaacgcct ctgacatacc tttgagcagt
     1681 tcatcaagct caccgtttac ttctattctt atcgcctgaa aggtcttttt gcacggattt
     1741 ttctccttgc gtaccttaag cggtacattt cttttgacta tttccgcaag ctcaagagtc
     1801 gtctttatcg gagcttcttc tcttgctctc aatattccgc caactatgcc atgagcaaat
     1861 ttttcctcac catactcaaa aagaatcttt tcgagctgtt ccttgctgta ttcattcacc
     1921 acgtcatagg cgctgagccc gctcttgctc atacgcatat caagcggtgc gtcctcgtgg
     1981 tatgaaaatc ctctttcggc tgtgtcaagc tgatacgacg atacaccgag atccatcagc
     2041 actccgtcga ccttttccac cccaagctcg tcaagacttt cgtcaaaggt cgaaaacttg
     2101 cggttaataa gtgtatgcgg atattctttc agcttttcat ctgccgcctt tatcgcatcg
     2161 ggatcgcggt caaagcctat aagtctgccg cttgtcaggc gctttgctat ctcagaggaa
     2221 tgtcccgcaa agcctgtggt gcaatctaca tatatgccgt ccggctttat gttcagcgct
     2281 tctatggttt cactcagtaa aacgggtatg tgaacaggtt tttcgctcat atttcctccc
     2341 gtttatattc cgagctctaa agcaagctct gtcagatctt cattgtttgt attctcattg
     2401 aactttatcc atgaagcctt gtcccatatt tccgccgtac cctcaagtcc tactacaact
     2461 acatcggacg taagcgcggc gtactctctg agcggctgag gaacagaaac tcttccctgc
     2521 ttgtccgccg atatctcata agcgctgccg aataaaaatc gttttatact ctgagttttc
     2581 acctgcggaa gctcatttat tcttgtgacc agcttctgcc agtcttcttt tgaatacacc
     2641 tttatgcagg ccgtacctat agttttggcg aggacaagat cgtttcccat ttcttcgcgg
     2701 agcttaagcg gtatattcat tctgccctta gcatcaagtg tagatttaaa ttcgcctatc
     2761 acgagcttgt ccctcctttc acaaaaagat acgccgaaga ggtgcggccc cgcacatttt
     2821 tcaaaaaagt tgaactttaa tgaaaaataa gtgtccaaag ccccaccaaa gtgtcaaaaa
     2881 gtgtacattt atattataca gcccacagca aaaaaatgca agtggaaatt ttatcattta
     2941 ttccgaaaaa gtagctaaat acagtttttt aatttagtat attttgcaca aaaaacaacc
     3001 cctcaaagag gggttgaatt cagtatttat tgttgatttt caacatattg tgctgtgcga
     3061 atttaaatgt actacatttt ggagaagtgg taaaaatgac atattgtgag cggatgggac
     3121 aatgtatgat atacacaaaa ccgccgggca ttgcgcccgg cggtcagctt gtagaaaaag
     3181 tccattttta taaaaatgga gcaaattctc tatcccta
//
LOCUS       DAWAOG010000211         3214 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3869_length_3214_cov_2.184552, whole genome shotgun sequence.
ACCESSION   DAWAOG010000211 DAWAOG010000000
VERSION     DAWAOG010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3869_length_3214_cov_2.184552"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          823 a          729 c          703 g          959 t
ORIGIN      
        1 ctcaagaaca ggaccgaatg cacctgctgt tccgtgtcct ccgaccattg gtatagaacc
       61 ggtacacata cctataagcg gatcgagtcc gagcagattt gaaagtccga gtgcggtgaa
      121 gttctgtccg attatcagca gtatgaccag tatcagaaat actatcatcg actttccgcc
      181 gcttttgagt actttaaggt ttgcctggaa gcctacagat gtgaagaaca gtaccataca
      241 gacttctttc agtgtatcat caaagctgac gtctgcaaga cccgtgccgt gcaggatgca
      301 ggtgagtatc gcaaacagca gaccgccaat aactggtgca ggaatgcaga acttttcaag
      361 aaaatgtatc tttttgcgca gaaatgcgcc aagcatcagg acaagcacag ctattgctac
      421 tgtctgatac atatcaagtg ctatgctcat cttgttactc cgtttctacg tttattccgt
      481 tttcgccgta atactcggcg gcacccggat gaaacgggat attgatgccg ttgacagctg
      541 attttctgtc ggttacgata tttacgggaa gtgcgttttg tatggtttcc gagttatcaa
      601 aaatcgcctt agttatcgcc tttaccgtgt ctttcgggca tgaattgctt gcaagcagta
      661 cagcggtaac gcctatagtc ttgacatctt ctgtctgacc gttgtaagtg cctgccggta
      721 tcgtgcaggc agagtagtag ctgtaggagc ttacaaggtc gtctatcttg tcgctgtcaa
      781 gcgaaacaag acgagtagcg cccttgtcgc agagtgattt tacagtagga ttaggtgcgc
      841 cgatagtgca gaaaaatgcg tcaatagtgc cgttttcaag ctgacttgcg gcatccttgt
      901 aatcaaggtt taccgtcttt atcatatcct cggcaatgcc gtatgaagtc agaatctgcc
      961 ttgcgttatg tgcagtaccg gattcttctt cgccgatgct tacagccttt cctttaagat
     1021 ccgaaacgct ttttatatca ctgtcactgc gtacagcaat ctgacagctt tcgttgaaca
     1081 gtcctgctac tgcgccgaaa tcacggtatc cttcggtaaa gccgtttctg ccgttccacg
     1141 catcgtctat tatgtcggtc tgtaccactg ccatctgaag atagttttcc gaaagcagac
     1201 gcatatttgc ggcagagcct gccgttcttt tgatattgat attgtagctt tcgagcgaat
     1261 ctatagcatt cagttcactg ccgaaaatgt agtaatttcc gccctcagcc gctgtgccga
     1321 ttctcacttt gttgcttagg tcgcagccgc cgcataaaag caatattgca ctgcataaca
     1381 gcggtatcaa gaattttttc atttttcacc tcaggttata ttgtataaaa aatattgtag
     1441 caagaattta ttgtaatgtc aagtagaaga agttgacggc tgaggggata tgtggaagaa
     1501 attttggttg gtttgttaaa atcgtaaaaa ttacagtgaa ataaacccga agacctctac
     1561 gaattttcac cgagaggtcg gttattagct ttcattcccc gagatagacc ttaaaattcc
     1621 tctcaagcaa cgttttcacc agctcctcgt tgctccttat cctgtgctgt gtttcaattc
     1681 cgtaggcact gtcaaagtcg gtgctgtgca aatcgcttcc tgcggtgata accgtatctt
     1741 cgttgccgaa aacacagatt gctttttcgt tttcaatatc acggtttcct ctgttgtagc
     1801 cctctattcc gtcaactatc gaaatatccg gtacttcatc gggatttttt atgtaatctc
     1861 tgtcacggaa agggtgagcg taagctaaaa atccgccgac tgagtggatt tcttgtgccc
     1921 atatattcaa atcggcattt ctcagaaacg gacgtgataa aagaaattcc ggttcaaagc
     1981 cgtaggctaa aaattccttg ccgttaccgt agccttcttc aatgccgaaa aacaggtcaa
     2041 aatcaagttc tgcggcgatt tttctgccgt tcgtgtatgc cttgctgtat tgcacaataa
     2101 gctgttccca cggcagagaa cgtgaaacac aggtgttgcc tcttataaaa tggtttgtta
     2161 caacagcacc cgaaaaaccg atttttacat actgctttat catatcttct atcgtatcaa
     2221 cagcacaggc actgccttct ttggtgtgca tatgcaattc gtatctgtac ataagaaact
     2281 ccttttgatt agatgaatgg tcagatgtta ttgtaacaca ttttgagagg ttttgcaaag
     2341 ggagagaatg tgcgggttaa gtaaagtcgc acagcagaaa tgcgaagcat ttttagtgtt
     2401 cgcccttgtg gaatactccg cattttgggt cggtggtcgg ttcattaacg attctttttt
     2461 tcggcggtct ggtgtcgtca aatggcttct tgccgtgcta ttcacggttt tcgttgattt
     2521 cctccatcag ctttctctgt ttgaaaaaaa tggtaaaatt actcatatcc ccttacattt
     2581 tccgctacag tagttttgaa gaatttattt gtagctgtaa gacaggttaa acacaccgcg
     2641 gcagaataga tcagaacaag taccgcgaca tcggttatcg ggaacattgt gatcggtgcg
     2701 tttgacaaaa ttccgaatgc aacgcaagct ccgccgctta tcactatgcc gagaataacg
     2761 gcgatgccga taccggtcag attttcacac agcagtaatt tcagcatttg attttttgtc
     2821 ataccgactg ctcttaacat accgaatatc tgctttcgcc cccacagtct gccatagagc
     2881 gaacctataa gcgcaattat cgaaaaaccg cataacgtaa agcaaaatat gcgtgaaact
     2941 gtcacaaacg agtcgtatac ctgctgtgct gagcttgtct gctgaataac gtccgtgata
     3001 ctaaggtaca taccctgcgc tttgtagtga gagtatatgt cgttcagcac acccgcaatg
     3061 agcgagtagt tgtccttatc ctttaccgtc aggtataccc tgttgtagct tgcgtcaagt
     3121 ccaagtgcgc tatatgcgtc tatcgaccat atatatccgt aaagcagctg tttcataaga
     3181 gagttttcgc ctgtgccgag gtctaagaca gcgc
//
LOCUS       DAWAOG010000212         3204 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3882_length_3204_cov_1.993331, whole genome shotgun sequence.
ACCESSION   DAWAOG010000212 DAWAOG010000000
VERSION     DAWAOG010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3882_length_3204_cov_1.993331"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          797 a          765 c          808 g          834 t
ORIGIN      
        1 ggtgtgtgcc gccgccggtg ctcttggtac agcggtaggt actgacgcaa acgcattcct
       61 cgcggcggct cttgcacctg cggcggctct cgcaggtatg ttttcgggcg ggaagaaaat
      121 aagcgcggcg ggagcgttcg tgctcaccgc gacaatatgc accgcctttt ttacaatgga
      181 taacacaaag cttgccgtgg cggcagatat ttttatgtcc tcggcagtat ttatgctcct
      241 ccccgcgaag ctcactgccg aacagaccga tatggtaaag ctgtcagccg ccgaaaacag
      301 tatccgcagg ctgttcgcaa aacgccttaa atttgccgag ggagctatcg cggaggtgcg
      361 gcggacggtc ggacttaccg ccgaaaagct cgacaatgtt ccctcgcgtg atataagctg
      421 ggtgtataat accgcgtgtg atgaaatttg cagaaagtgc agatataata tgcagtgctg
      481 gggaaaggaa tacggcgaca gcataaagca gtttgccaag cttacaaatg ccgttaaaag
      541 cggcgaagag cccacaccgg agctgttttc cgcccccttg tcggagcgtt gcccccacaa
      601 ggaacagcta tgcaataaga tcaataagct ttacaccacg ttgtcaaata atgaagccga
      661 gcgcagaaga atagcgcgta tgcgttccgt ccttaccgct cagttatcgg caacagagca
      721 aattctatcc cagttatcgg acgaaattga aaacagcggc gaaattgagc ccgaatacaa
      781 cgaaaccgcc gtcaacgtcc tgtacaagct tggctgtgag gatgcccagg cggtgagcgt
      841 tcagctcggt gagcaaggca gaatgtcggt agaagcctat tctgatacag gcttctttgc
      901 ttcaaagcag gacatctgcg aggcgctgac gcttgctttc cgccgccgct tcgacctgcc
      961 gacgataagc cgcgtgggcg gcggctgtaa gctcagcgtc ttctcgcaga ccacctacac
     1021 gcttgatata gagatatgtc agctcagcaa gacggagggc actgcctgcg gggattatta
     1081 cgagagcttc atcgacaaaa gcggcatagc ctatgtagtc ttgtcggacg gtatgggtag
     1141 cggaagcaga gcgcgcgttg attcgtcctt tgcctgcggt atgctgatac ggcttttgca
     1201 ggcattgacg cggcgataaa cgtcataaac acctccctta tgtgcaagtc ctccgatgag
     1261 agcttcgcca cgctggaaat atgcgccgtt gacctctaca gcggtaagac cgatctgtat
     1321 aaggcgggca gtgccaacac ctacatcaag tgcggaagcc gctgtatgcg ccttggctgt
     1381 aaggggcttc ctgtcggcgt aaaggacgag cctagctacg accgcaggac tttcacgata
     1441 ggcagtcgcg atatgatagt aatgacaagt gacggcgcgg agcttaatga aaaatggctt
     1501 taccgcgaaa tggacaaaga gcccgactta aaggagtttt ccaaagaggt tgccgctacc
     1561 gcaaggttct atgcgggaga agaaaaaagc gacgatattt ccgtaatagc catgcggctg
     1621 agtaaatagc aagtatccgc ataaatagta ttgaccgcgg cgctgtcggg tgatagaata
     1681 tatagcggtg ctgtccgttt aagacagtgc cgcttgattt tttgttaaac gaggttttta
     1741 tgaaagcgga tacccttaaa aagacatata tcgcggcgtt gctcgccatg ctctgctctg
     1801 cgctctgggg cagtgcattc ccctgtgtta agataggcta cggactgttc tcggtaaaca
     1861 cggaaaattc cgcgtcactg atacttttcg ccggagtaag gtttttcata gccggaatta
     1921 tggttatcat aatcggcagt attatgcaca aaaaaccgat gataccgaag cttcgcgaag
     1981 taccgaaggt tatctccctg agcctgttcc agacaattct gcaatatctg tttttctaca
     2041 taggacttgc caatacaagc ggcgtaaagt cctccgttat cgagggcatg agcgtgtttg
     2101 tctgcattct gatttcatca ctaatattcc gcttggagaa gctgacatgg cttaagataa
     2161 tcggctgtgt gctcggcacg gcgggtatta tcgttatcaa tctcgacagc tcgctgctca
     2221 gcggcttcaa tcttacaggt gacgggttta tccttatatc gacagtcgcc tatgccctgt
     2281 cctcggtgct tataaagcgt ttttcggtcg ataccgacac catgatgctc agcggctggc
     2341 aatttctgtt cggtggtgcg gtaatgaccg ctataggtct gatcgccggc ggaagtctga
     2401 gcatttccgg cagtccggca cccgctgtgc ttatgctcat ttatcttgca ttcatctcag
     2461 cctgcgcata ttcgatatgg agtaccctgc tgaaatataa cccggtatct aaaattgcgg
     2521 tgttcggctt tatgaacccg gtgtgcggag ttctgctctc tgcacttctg ctcggcgaag
     2581 ctcagcaggc attccgcccg gaagcgctta tcgcacttgt gcttatcagc ctcggaattt
     2641 tcatagtcaa caaaggacag gaacaaaaag actgacaact taagccttat tgttataata
     2701 gagcagcttt atacaatttg tatttaaaac agcttttgtt tttcataaaa aagtgagatt
     2761 tttttaaaaa agtgcttgac atcaaggttt tgttgtgata taataatcta cgttgtcacg
     2821 acagtagcaa caaaaccgaa tattggggtg tcgccaagtg gtaaggcatc ggactctgac
     2881 tccgccattt cgagggttca aatccttcca ccccagccaa ataccattta aaccgcattg
     2941 ttatgcggtt tattttttta ctacacgaat tttacacgaa ttattttaaa aaaattttat
     3001 caagtatcct tatagagcgt tcttcctctc tcgggtaaag gcgagcatac gtttctaata
     3061 tttctttgat actgctatgt cctagtcttc tcgctatctc ttgaacattt atcccctcgt
     3121 ttattagcaa cgttgcgtgt gtgtgtctga aatcgtgtac tcttatgtgt atgtgcggcg
     3181 aaaacaaagg ctttgtacgc aaat
//
LOCUS       DAWAOG010000213         3203 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3884_length_3203_cov_3.092122, whole genome shotgun sequence.
ACCESSION   DAWAOG010000213 DAWAOG010000000
VERSION     DAWAOG010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3884_length_3203_cov_3.092122"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          916 a          632 c          892 g          763 t
ORIGIN      
        1 ggctttacct gtgcggacga aatgctttat cccgaaaatg accgctatct gtcggatctg
       61 ctgtcatatg ttgctgtcgg tgcgcgctct gtcgaggatc agcagcacag actcactgca
      121 agcggacttg atatacctgt gggaatgaaa aatccaacca gcggaactat gtcggtaatg
      181 ctcaattcta tccgtgcggc gcaggcaagc catactttta tatacagagg ctgggaaacg
      241 cacacaagcg gaaatccgct tgcacacgct atactgagag gtgctgtaaa caagcacggc
      301 gaaaccatac cgaattatca ctatgaggat ctttcgctgc tttatgagct ttactgcaag
      361 caggagcttc ttaacaaggc ggtaattgtt gatacgaacc attctaattc cggcaagaaa
      421 tatcttgaac agataagaat tgcaaacgaa attctgcaca gccgccgtca cagcaaggat
      481 cttgagggat ttgtcaaggg acttatgata gagagttaca ttgaggacgg aaaccagagt
      541 acggatgacg ctgtttacgg caagtcgata accgatccgt gtcttggctg ggaaaagacg
      601 gagaaactga tttacagtat agccgaacag ctgtaagcgg cgggtcgccg ctgactgttc
      661 cgacctttga taacgttgcg gattttcgca ggatagcggg acggtcgggg gttagcgatg
      721 gtgctatgca gattgtcagg tgtccgtgag gcgcgacaaa ttccgccttt taaaaaggtg
      781 gatataggct ttggttgcgt tgacggcggg cggcaatgtg accctgattt acaatttgat
      841 aatgttgagg atttttgcgg gatagcggga cggtcggggt ttagcgatgg tgcaggatca
      901 atttgaggct tgttgaaagt ttgggcgaag aactatcaca tttagctcca ttgtgtaaag
      961 aaagctggca cagtgcggta taacattttc aatgttgctt tatatatcag gttaacgatg
     1021 ctttgttgta gaactgagtt tttcgtcact gtgactgagg gattgtcagc ggaggttata
     1081 gtacaagcgg aaaggttcgg tgtaaacgat ccctcaatag ttggctgagc ttgactcagc
     1141 aacagaagct cacctttggc aagggacggg caaggatgcc cgatattgaa cgcccagagt
     1201 ttaagcatgg atgcttataa gtggtcgaac caagtcggtg caggacgcac cgaccacaaa
     1261 cgaccacgat tgcagagacg caaaacaaac agagcgttca taagccgaga attagtgcaa
     1321 atgggtaaat aatgctccta tcagatgaca gcatgatatt gcaaacggac acacagacgt
     1381 ttgtctaaaa ataataaaat caaaataaac cgaaaggaag aagaaaatga cactttacga
     1441 agaacttatt gaaagagggc ttatcgctca ggttacgaat gaggaagaga taagcaagat
     1501 gataaacgag ggaaaggcta cattctatat cggctttgac ccgacagcgg attctctgca
     1561 tgtcggtcac tttatggcgc tgtgccttat gaagcgttta cagatggcgg gtaacaagcc
     1621 tattgcgctt ctcggcggcg gtacgggtat gataggcgac ccttcgggca agacggatat
     1681 gagaaagatg ctcacaaagg aagatatcga gcataatatt gcctgcttta aaaagcagat
     1741 gagccgtttt atagatttct cggacggcaa ggcactgctt gtaaataatg cggactggct
     1801 gcttgattta aactatgttg acgtactgcg tgaggtgggc gcttgcttct cggtaaacaa
     1861 tatgctgaga gcggagtgct acaagcagag aatggaaaga ggtctttcgt tcctcgaatt
     1921 caactatatg ataatgcaga gctacgactt ctacaagctg tacaaggatt acggctgtaa
     1981 tatgcagttc ggcggcgacg atcagtggag caatatgctc ggcggcactg agcttatcag
     2041 aaagaagctc ggcaaggacg cacacgctat gactattact ctgctcttaa attccgaggg
     2101 caagaagatg ggtaagaccg aaaagggcgc tgtatggctc gaccctgaaa agacaagccc
     2161 gtttgaattc ttccagtact ggagaaatgt tgcggacgct gatgttatga agtgcataaa
     2221 gatgcttaca ttcctgccgc ttgaacagat aagagagatg gagagctggg agggcgcaca
     2281 gctcaacaag gctaaggaga tactcgcata cgagcttact aagcttgtac acggcgagga
     2341 agaggctgaa aaggctttag ccgccgcaaa ggagctgttc ggcggtaagg gcgtcagcgg
     2401 tgatatgcct actgctgtta tgcctgccga gcttgtaagc gacggtaaga tcggtatact
     2461 tgatgctctt gttgcgtcaa agctctgccc ttcaaagaga gaggcaagaa caaacgtaac
     2521 aggcggcggt atcagcgtaa acgatgaaaa ggtgaccgat cctgcggcta tgatagagat
     2581 aggcgaattt gccgtgataa agaagggtaa aaagagctac tgcaagatag taaaggcata
     2641 aggtgtggtt acagtaagaa gagcgcagga caaggatatt aagcgtgtta cggagttgct
     2701 cggtcaggtg ctggagcttc acgccgattt aagacccgac ttattcatac acggtacgac
     2761 aaagtacacg gaaaatcagt tgtcggatat attcgctgat gaaaggacac ctgtatttgt
     2821 ggctgtagat gagagtgaca cggtacaggg atatgcgttc tgtatattca aagagcctgc
     2881 ttttgcaaca aatatgacag atataaaaac gctgtatata gatgacttat gtgttgacag
     2941 caacgcaagg ggtcggcata taggaactac gctttttgac tatgtgatgg aatttgccaa
     3001 ggaaagcggc tgctatactg tgacgctgaa cgtctgggag ggtaacgaca gcgcaaggca
     3061 tttctatgag aaaatgggaa tgttcgtccg tgaaacccag atggaacggc gggtggcggg
     3121 tcgccactga caattcgccg ttctgatagt tggcaggttg cttaacttcc gttaccggcg
     3181 ggcggcaatg tgccgcttca aat
//
LOCUS       DAWAOG010000214         3194 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3900_length_3194_cov_2.301688, whole genome shotgun sequence.
ACCESSION   DAWAOG010000214 DAWAOG010000000
VERSION     DAWAOG010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3900_length_3194_cov_2.301688"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1951..1960
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3025..3034
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          802 a          844 c          652 g          876 t
ORIGIN      
        1 ctgcctcgaa ccgcacctac cttacccccg atggcgaccg ttattataaa gacgattcgt
       61 ggaacggcgg agtgttctca agctttgccc tgtcagcgtc tgacagggaa ttattgcatt
      121 cgtcggatat ggtgcatacc accttttccg gaccgaattt caacgatgta atagactgct
      181 gccgtgaaaa gcgttttccg ctgagcgttg attttgatat ttgcagggat tttgacacga
      241 tagaaaaata ccttgactgc atatcgcttt tgtttataag cggagatgaa cctacgcttg
      301 aaaaagcgaa acggctttcc gcaaaatatc ccgacactat attcattgta acgctcggag
      361 aaaacggaag caccgccttt tcaaaagaca cggcttatca ttgtgccgcc gctgaggtat
      421 ccgaagtaac agataccacc ggttgcggcg acagctatca ggcaggcttt atagcaagct
      481 atcttgctga caaaaacata gaaacggcta tgagcaaagg ctcgcagaca gccgcagaaa
      541 cgctttcgtt catcggagga ttcagatatt gaaatacggc ggaaaagcaa aagcttttcc
      601 gccgtcatta ttttatatac tcacctcgac cgatacgcac ttgccgccat acagcttttc
      661 gcttagctgt gtcggctgtg tgaagcctgc gtacagcgta tatctgccgc ttctcaccgc
      721 tcttgcgccg ttatcgtcaa ccgtttcaaa cgcactttcc ggaatattga catttatcgt
      781 cctgctttcc ccgtcaaaca gcgaaacacg ctcaaatgcg caaaggctgt gatttttcac
      841 cccgttttcg ctctttatat agacctgcaa aacgtcctcc gcacttctgc ttcccgtatt
      901 ggtaacatta acggtcagca tattgtcctt gtacgaaaca tccccgcatt caaagtgcga
      961 ataggtaagg ccatagccga acggataaag cacgttgctt tcatcgtcac agaagcgata
     1021 tgtcctgttc ttcattgaat aatcggtgaa atcgggtaac atatccgccg atttgtagaa
     1081 agtgaccggg agcttgccgg aaggcgaaac ctccccgaaa agtatctcgg caagcgcctt
     1141 gccgccgtac tgaccgggat accatgcatt gataagcgca ttaccctcaa attcagtatt
     1201 tacactgctt cccgccgcaa gaactattat aataggcttc ccggtgtttt taagcttttg
     1261 cagcagcaca cgctgtacct ccggcagtct taaatcgggc ttatccccgg atgcaaactc
     1321 atttcctgtg tcgccctcct caccctcaag agtagcgtca agtcctacac atacaacagt
     1381 aacatctgcc gcttcacaag ccgcgaccgc ctcggcatat ctgtcgccgg ggagcgcaag
     1441 tccctgcgtc ctgtcgcgga aaagctgaca gccctccgca taatataccc tgccgtcaaa
     1501 cgcgtcctgt attccctcaa ggaacgtaac gcttctgtct ggcgtaccgt tatagtttcc
     1561 gagcaacgcc gcacggctgt ctgcattagg accgatcacc gctattgagc ttatcttgtc
     1621 acgatcaagc ggcagtatac cgtcattatg caacagcacc attgactttt cagccgcttc
     1681 aagtgacagc gccttgttgc tgtcgcaggc gataatatca aacggcaggt cgtcaaattc
     1741 gttatcgtca agctgtccga gccttattct tgtgcgcaga gcatgaatgc aggcagtact
     1801 tatatcttcc tttgttataa gaccctcttc attcattttt ttaattatat ctgcaattag
     1861 ttgcattgaa tatcttgttt tatcttctct atacattatt gacagtttgc ttgtgatttc
     1921 gacaaattct tcagcaattg tttctgtttt nnnnnnnnnn ctcttcaaga gccgcaagta
     1981 tatggagata agtatttccg caattgacat cacagccggc tttaagcgcc atagccgccg
     2041 actgcggtgc agtatcggta attttatgtg ttgtatgaaa gtcccttatc gcccagcaat
     2101 ccgacacgaa atagccgtca aagccccact cacggagctt ttccataagc ttttcagacg
     2161 cgcacgcagg ctcgccgttt accctgttat aagcacccat tacgccctcg acatttgcct
     2221 ctttgacaag cgctttaaat gccggcagat aggtttcttc catgtccttt tcagttgcct
     2281 tggcatcaaa ctcatggcgc agtgactccg gaccgctgtg aactgcaaaa tgctttgcac
     2341 acgcggcggc tctgatgtat ttttcttctc cctgtatacc ctttacgaag ctcactccga
     2401 gccttgaagt caggtacgga tcctcgccgt aggtttcatg ccctctgccc caacgcgggt
     2461 cacggaatat atttatgttc ggtgcccaca aggttagacc tttgtatatg tcggtatcac
     2521 cgtgcttttt tgcactgttg tacattgccc tcgcttctgt ggaaatcacc tcaccgacac
     2581 gatacatcat atccttgtcg aaagccgccg caagtgcgat agcctgcgga aaaaccgtag
     2641 ccgtgcctgc tctcgccact ccgtgcagac cctcattcca ccagttataa gacggcagac
     2701 ccgctttttc gatcagcggc gcgtcatatt tcagctgctg cgcctgctct tccgttgtaa
     2761 gcgtatcagt aagtgctgct gctctttcat aagccgaaag atttttgtct ttatattttt
     2821 ccatattgtt ctccttaaaa agccgatgct tttgcctgtt tttctgattg taacataatc
     2881 acgtcccaat ttcaagacaa aaataaacgg aactgtgcca taattcctca gcttatcaaa
     2941 ttctatcaag cttaaatgca atatattgta aaaaattcta aataacagac atacagccaa
     3001 caacgccgaa aacagttgcg atacnnnnnn nnnngtgcgg ataaaaaaca acttggcagt
     3061 ataatagaat atatattgcc aacaacgctg aaagcagttt cgatatcgac aaggcgttaa
     3121 taatttcgtg agtaaatccg tgcaactgca ctatcaccga ctatcaaaaa agaacgctac
     3181 tatcctgaga tatc
//
LOCUS       DAWAOG010000215         3173 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3928_length_3173_cov_2.053881, whole genome shotgun sequence.
ACCESSION   DAWAOG010000215 DAWAOG010000000
VERSION     DAWAOG010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3928_length_3173_cov_2.053881"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          957 a          690 c          872 g          654 t
ORIGIN      
        1 gagatacccg atatgggcgc ggtaggtatc atcacctata tgcgtaccga cagcctgcgt
       61 atttcggacg aggctaaggc ggccgccgct cagatgatag aaagcacata cggtaaggaa
      121 tatctgccat caaagcccag agtgttcaag tcaaagaaca acgctcagga cgcgcacgag
      181 gctatccgtc ccacaataat cacgcttact cccgaaaagg taaagtctgc gcttacaagc
      241 gaccagtata agctgtataa gctgatatgg gagcgcttta tggcaagcca gatggaaaat
      301 cagctccttg acacaaaggc agtcgatatc acctgcggag agtgtttgtt caaggcaaac
      361 ggatacaccg taaaatttga cggctttaca aagctgtatg aagaaagcaa ggataatgac
      421 gaagaggagg gcggcgcact gccggctctt gaagtcggcg aaaagctgaa agtaaaagag
      481 cttacaggca atcagcattt cactcagccg ccaccgaggt atacagaagc aagccttatc
      541 aaggcactcg aggaaaacgg cataggcaga ccgtctacct atgcgccgac aatcgctaca
      601 atacttgaca ggcactatgt cgagcgcgaa gcaaagcagt taaaaccgac ctcgctcggt
      661 atagtgataa ccgaccttat gaagggtcat tttgagcgta tagtggacgc aaagtttacc
      721 gcacagatgg aaagcgacct tgataagatc gaagcgggaa aggccgactg ggtcgatgta
      781 ctcggaaagt tctacacagg ctttgacaag atgcttacaa aggcagaaaa ggatatggag
      841 ggcaagaggg tgaagatacc cgatgagcct accgatatag tctgtgacaa gtgcggtaag
      901 cctatggtca taaagatagg tccttacgga aagttcctcg gttgctcggg cttccccgag
      961 tgcaagaaca ccaagaggat agtaaacgaa acaggcggac tttgccccca ctgcggcggc
     1021 aagatgcttg ccaagaagtc aaagaagggc aagcctttct tcggctgcga gaactacaag
     1081 gactgcaact ttatgacatg ggatacacct cttgcggata agtgccccaa gtgcggctcg
     1141 acgctgttca aaaaggtcgg caagcagggt caggtatact gcgcaaagga cggttgcgga
     1201 tatgtacgcc cagccgatga ggataagaaa aacgactgat aaggatagat aaattggata
     1261 aaaataccgt caccgtgata ggcgcgggtc ttgcgggggt tgaagcggca tgggcactcg
     1321 caaacagagg ctttcatgta agactttgcg agatgaagcc cgaaaagtat tcgcccgccc
     1381 acaagtacaa gggctttgca gagcttgtat gcagtaattc gctgaaaagt gccgacactg
     1441 ccagtgcctg cggtatgctt aaggaagaaa tgcgctatat gaattctgtc acgatgatcg
     1501 cggcagagaa gacaaaggtc agcgcaggcg gtgctcttgc ggtaaaccgc acggagttct
     1561 ccgacgctgt gaccgaaatg ataacaaatc acccggatat agagatcgta agcggcgaga
     1621 taaccgagtt ccccgaaagt aacaccgtaa tagcaacagg accgcttaca agcgatattt
     1681 tcgccgaaaa gatacagcag cgttgcggca gtccgctcag cttctatgac gcggcggcac
     1741 ctatagttac agcagaaagc attgatatga gccttgcgtt ctttgcaacg cgctacgaca
     1801 agggcgaggc tgactatatc aactgcccga tgacaaagga agaatacgaa gccttttata
     1861 acgaacttgt aaatgccgag agcgtacagc tgaagagttt tgaaaatctg aaagtgtacg
     1921 agggctgtat gcctgttgag gtgcttgcaa agcgcggtat agaaagtgtg cgttacggct
     1981 gtatgaagcc tgtcggtctt accgacccga ggacaggcag acgaccttat gcggttgtac
     2041 agctcagaaa agaaaacaac gagggtacgc tttataacct tgtgggattt cagacaaacc
     2101 tgaaattcgg cgagcagaag agagtattct caatgataac aggacttcag aacgcggagt
     2161 ttgtaagata cggtgttatg cacagaaaca cattccttaa ttctccggga ctgcttgaca
     2221 agaattttaa gctgatcgac agcaataata tctactttgc aggtcagatg acgggcgttg
     2281 agggatatgt cgaaagcgcg gcatcgggaa ttatcgcggg aataaacctc gcaagggcgc
     2341 ttgacggcga aaaaccgctc gaactgcccg aaacctgtat gacggcggcg cttgcaaagc
     2401 atataagcac agagaataag gactttcaac ctatgggtgc gaatatgggt atattacctc
     2461 cgctccccga gaggataaag gacaagaagt taagatacag gacggttgct gacaggggtc
     2521 ttgcagatct aagaaaagca ctctgcgagc agggtgttat caaagagtaa aaacagattt
     2581 taccttaacg aaaggaaaac ggaatatgaa aatagctata gacgcattcg gcggagataa
     2641 tgcacccgac gaggtagtaa agggcgcggt agccgctgta aaggaatacg gtgtcgacat
     2701 tgtactgaca ggtgacacaa agaagcttga cgagtgcttc acaaagctcg gactttccaa
     2761 ggatcacatc acgtttgagc aggctgacgg agttatcgaa atagaggata accccaagat
     2821 gatattaaag gaaaagaaga actgctctat gggcgtggct atgcagatgg tagctgacgg
     2881 cagagcggac gctatgataa gcgcaggcag tacggcggct cttgttatgg gcggtactct
     2941 tatagttaag cgtatcaagg gtgtaaagcg tccttcactc actcccatta tgcccggtct
     3001 taataatatg tatatactgc ttgacggcgg tgcaaacgtt gactgccgcc ccgatatgtt
     3061 aagacagttt gctgtaatgg gcagtgtcta tatgaacaag atcatgggtg ttgataaccc
     3121 caaggtcgga cttctcaatg tcggcgcgga ggaagaaaag ggcagagagc ttg
//
LOCUS       DAWAOG010000216         3169 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3937_length_3169_cov_1.971098, whole genome shotgun sequence.
ACCESSION   DAWAOG010000216 DAWAOG010000000
VERSION     DAWAOG010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3937_length_3169_cov_1.971098"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1509..1518
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2163..2172
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          990 a          587 c          735 g          837 t
ORIGIN      
        1 gctcaatgat gctgttaaaa agggcggcgt tatggcatcg tcaagcgtag gcggtctttc
       61 gggtgcattt atacccgtat ccgaggacgc aggtatgata gatgctgtaa accgtggcgc
      121 attatcaata gaaaagctcg aagccatgac cgccgtttgc tctgtcggtc ttgatatgat
      181 agccgttccg ggcgatactt cagccgatac gatctcggcg attatcgcag acgaagccgc
      241 tataggtatg gtaaactcaa agacaacggc agtccgtgta attcccgcaa taggaatgaa
      301 ggaaggcgat acgctggaat tcggcggtct gtttggaagc ggacctgtaa tgaagctgag
      361 taagttttca tcttcaaagt tcatttcaag aggcggacgc attcctgcac ccttacaaag
      421 tcttaaaaac taaccatgaa ccgcaggcgc accctgcggt tttttgatgc gtaataatgc
      481 gtttcgcagg caatttaatt ttgtcaagga tattttcgta caaaattttc gcgggctttt
      541 tgtttattat tttgcttgta ttttggggat aattgtagta aacttaatta tgattttaga
      601 tttataagca gctttcagaa aggtggtgct ctgatggacg ttatcagcga aattatcgct
      661 tatgaaaagc acgctgaaga aatagtggcg gacgcgaaca aaaaagccga tgctattctt
      721 accgatgcgg ctgtactccg tgaaaaagaa cgcataagct atgaagagga tcgcagtgat
      781 aagataaaca gctatcacga cgaactgaaa aaaagcggag aagaacacta cagcgcgacc
      841 tcggcagagt gcagtgctgt gatagcccgt cttgatgatg atttttcaaa aaacaaggac
      901 aagtgggcgc aggagatata cgataatata gtttcctcga agtgacagca caaaatatat
      961 atccggaggt gatattatgt cggcgggttc ggcggcggta atagccaagg caagagccaa
     1021 atacggcaag cttcttagtc ttgagcagta taagaccctg atatccaagg cgaatgtcgg
     1081 cgagatagta acacagctta aagcatatga cgattttgac aaggattttt ccggtgtaga
     1141 caataccgta aggagaagcc aggcagaaag gttaatggaa aagcggcttt tcagagtata
     1201 tgacgagctc agaaaatttt gtcccggcag caaaaacagg ttttatgatt ttttgctgat
     1261 acaggaggaa ataaaacaga taatcaatgc atcgatgtat atcggagcag gcgtttatga
     1321 tctttttata ccgggttttc cgggatatct tacagatata tgttcttatg atatcctctc
     1381 actttctaag gcgcgaacat atgatgagat actggcagtg cttaaaaaca cgccttatta
     1441 cgatgttctt atgccgctgt ctgacggaac gagagctttt ccgccgatag tatccattga
     1501 ctgtgcacnn nnnnnnnnca aaatatcttt acacaacgct ttttgcatat ataaaaaaga
     1561 gcgtatccgg aagcgaaaag gctgaagttg aaaagtgtat acgcagacgg tgcgatatgt
     1621 acaatataaa aatatgttac aggcttaaag gtctttttaa aatgtcgaca gaagaagttg
     1681 tagctcacgc attgcctttt tatggcaggt tcgataaaaa tacgatggaa cggattttga
     1741 caaaagccga taacgagcct atactgcctc tcctgcttaa gcttccttat tttaaggata
     1801 tcaacgcaga acaggaggta gacattgaaa cagccgtcta tacatctaac aagcgttatt
     1861 acgacagcaa gctcgcatta tcacagtgcg acagcacagt catatatgct ttgatcgagc
     1921 ttttgcagat agaaaaccga aatttaacaa ctgtgataga gggcgtaaga tactcgcttg
     1981 aaccgtcaca gatagaaaaa atgctgatct tataaggcaa atatacagcg aaaggaatga
     2041 tataatggca ggtttccacg tggctgaggc cacaagcaca gtcagcctga atattgcttt
     2101 ggctgacgca ctttcacagt atcccgagct tttcagggaa aatgtgaaaa tagcttcatt
     2161 ttnnnnnnnn nncacttata tcggtcagcg gaccactcaa aaaagtcaat aaaacgcttg
     2221 tgcgatgttg tgaaacaaag tgctttcaca ttgaaccgtc gttttacaca atgacttacg
     2281 gttctgctca tttcaagacg ctaaaagata aggatatcta cggcggactg atgaaacgag
     2341 cggaaatatt atcatcggga cttggtatca aagaaaaatc cgttccttat gacgatatac
     2401 aaattgaggc tatacacgat tttgatatgt attttaccga gcttgaacaa caggtaatgc
     2461 ctcttattga aaaaaagaac gagcttgaaa ggtcgataga ggagtatcgt caggtattaa
     2521 gacaggtcgg taacctttca aatataaacg ttgacttcaa ggatctgttc ggatgtaagc
     2581 acgtcaaaat acgtttcgga aggcttccgt ctgagagcta cacaaagctt gaattttaca
     2641 gtgaaaaagg cttcgtgttc gagccgcttg aagaaaagga cggttatgtc tggggacttt
     2701 attttgcacc tgtagaggaa agtgtacaga tagacgatat tttcaaaagc ctttattttg
     2761 agcgcatccg tcttcctgac tttctgcacg gcgacggtga aacagcaacg gcagagctta
     2821 accgacagct gaaagatttt gatgtcaagc ttaaagaggt caacgaaaaa ctggctacag
     2881 taaagcagtc cgaggaaaag caatttgaaa aggtacgaag taagctcata tttcttaata
     2941 acagctatga gctgagaagt caggtatctg taatcaataa taaattttat atggcggggt
     3001 ttgttccgac aagagaagtc gagaagttca gaaagcatct ttcgactgta tcggatattg
     3061 ttattgaaga aaaaaaatat ttcgttgacg acagaatgaa acctcccaca aagctcagaa
     3121 acaattggct gttcagaccg tttgagatgt ttgtcaatat gtacgggct
//
LOCUS       DAWAOG010000217         3169 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3936_length_3169_cov_2.153179, whole genome shotgun sequence.
ACCESSION   DAWAOG010000217 DAWAOG010000000
VERSION     DAWAOG010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3936_length_3169_cov_2.153179"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          920 a          625 c          757 g          867 t
ORIGIN      
        1 cgcagaatgg gcgcggatat cacagttgac agcagagtag cggtagtaag aggggttgac
       61 cacctcacag gcgctcctgt caaggcgagc gacttaagag ccggtgcggc gcttataatt
      121 gcgggacttg cggcacaggg aattaccgag atcgaggata tacaccatat tgagcgcgga
      181 tatgaaaata tggagataaa gcttagagag ctcggagctg atatagtaaa gcgttactat
      241 cccgatgacg agaagatgag aaaggcactc tgatggcaaa ggttgtacct gtatgctcgt
      301 ccagcaaggg caacagtata tttataggcg atacttcatc gggtgtactt gtagatgcgg
      361 gctgcagctt caaggcattg aaggacggac ttgcgctgtg tggcataccg tttgaagcgg
      421 tgaaggcagt gctgataaca cacgagcata tcgaccatac aaaggcgctc ggacagataa
      481 caaagcgtac atcgcttcct gtatatgcgt ctgacggtac gacaagaaag ctttttgagg
      541 ataaccttgt tgcaccgggt gcggtcatac attctttatc cgaaataggg agtgtgcccg
      601 ttgatatgga tattgatttt tttcacacgc cgcatgatgc cgccgagagc gtgggatata
      661 cggtaaagtt caaggagcac aaggtagcgt gctgtaccga tctcggatat gtaacagacg
      721 aggtaaaaag aaatctgatc ggctgtgata cggcatatat agaagccaac tatgacccta
      781 agctcctttc ggtcaatccg aaatatccgc cgtatctcaa aacaagaatt tcgggtgatc
      841 gcggacacct ttcaaatatg agttccgctg agctttgcac cgagcttgta aaaagcggtg
      901 caagacggct gatactcggt cacttaagtc aggaaaacaa tactccgaaa atagcatata
      961 acgcagttga gcaaaagctc aacggttgtg gtgtaaaatg cggagttgac tgtacacttg
     1021 atgttgcgcc tgtcgtaacg cagggaaaat acataattat ataatcgctt gatatgcact
     1081 gcattttctg cttggtggta actttgcagt tgtgccgtgc tttatttttt ttattgtgaa
     1141 aggatctatt tatggcagta tatcttatac ttatggcgat agttctggtt ttggcgtatc
     1201 ctcttattga gcgcaaaccg agcatcggga aaaaactctg ctatgtcatt gtgacatttg
     1261 cggctatgta tctgatcagt attttcaggt acggactcgg taacgactac tattcttaca
     1321 tctatatttt cagaaatata caggactcat ccgggcttgc aattttcaac ctcggatatg
     1381 agcctgcgtt tactgtaata acaaagctta tttcactgtt tacctcaaat ataaatgttt
     1441 tgtatgctat atacgcgctg cttatacttg tgccgacggc gtatgccata ttccgataca
     1501 gcgagaatat atggatgtct accatgatgt ttatttcgct gacattcttc tactgctcac
     1561 tcagctttat acgccagtcc atcgcgtttg cggtaatatt gtgtgcatat aagtatgtaa
     1621 aggaacgcaa ccatttcaag gtgttgctgt ttatattcat agcttgtctt ttccatagca
     1681 cggtaattgt gatgatacct atctatctta tcgccgcgtt tgtaaagcct acaaaaatta
     1741 cagttccgat atacggtgta attacagcgc ttgtatattt cctttcatgg cctatactca
     1801 gacttgccgt tcttatactt ccgcagtata agggatatct tgacctcaac ttcattactc
     1861 agggatacaa acctgtgtat ctgatagttc cggctataat tgccgcactt gctattgctg
     1921 cacattttac aggatacggc aaggcgtatc cgaagcaatc atcgattttt acgaattttg
     1981 ctattttcaa ttttatcata tggttcattg ccacaaagca ctttgtcata gaacgtttct
     2041 caatgtacat ctacattttt atgataatgt ttattccgtc gatcgcaaga tattatatga
     2101 attgtgctaa ggtttatttc gcgaaaaaga aagatcccga ggcggtcgtt gtttttgata
     2161 aaacggtaga cgaggtctta gcagagaaaa agtcggctga cggtgtagag ccggctcagg
     2221 agtctatcag taaaataacg gctgaggaag ctgatgtgcc gctcagcgag gaagaggctg
     2281 aaaaacagcg tatactggct gaaataatgg ctgaggacgc agaagaaaac attccggaag
     2341 atacggatat caccgaagag gaagatccgg aattactgac cgaagaaatg accgaaacag
     2401 atgaaccgga agaaaatgcg gtatatacaa aaagaaagcc cggttcggcg ctgaattggg
     2461 acgttgccgg tgatgaacgc tatctggcgg aaaacagaga gtttaaaaac agaagcaata
     2521 agttcctgca atttatttcg cgtcctgtta cgatatttgc cgcatttatg gttgtggtcg
     2581 tggcatcaaa tctgtggtat aactatttcg gacttacagt ttcccaaaaa ggcttccacg
     2641 gagtaatgcc atataagagc actatacccg cgtacactga gctgatgctt tcaacagaag
     2701 acaaggataa taaaaatgaa ttgctcagag atgaagagaa cttccttaat tatatgtacc
     2761 gtctgaaaga aaatgataac tattcggtta tcatttccgc gagaggagat acagtcggag
     2821 gtctgaatga cggtgcgcgt tccgcactta aggagcttgg acttgtaaag cttcccgaag
     2881 ctgaatcggc agacagatat gtagctgtta tcgaaggcgg aaaggtcaca cgcgaggaaa
     2941 tatcggacag tgctgatatt gacacaggcg aatttcgtgt gctcggcttt aagacgcacg
     3001 ttatcagcac acagcgtact tcaacgatac agatgggtaa aaagaacttc tcgctaaatg
     3061 agcgcggcat gaatatagta gtgcttgata atacgacaaa aaagatcgtt gacaaggttc
     3121 gcttcaaaac atactatgta atgcttacag caaccagata aggtggtag
//
LOCUS       DAWAOG010000218         3160 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3953_length_3160_cov_1.919485, whole genome shotgun sequence.
ACCESSION   DAWAOG010000218 DAWAOG010000000
VERSION     DAWAOG010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3953_length_3160_cov_1.919485"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    711..720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2152..2161
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          851 a          681 c          648 g          960 t
ORIGIN      
        1 tatcaacact gttcggtacg gacatctata tgtccgtatt cttcttcaag aattgtaaga
       61 tgtgtgtaaa aggatcttaa catagtgcag gcatcatcaa cataaggagg tttcagcctg
      121 aactttgcat aaccgtcttt ttcggagctg atatcaatat cggcggctac actgaaaaaa
      181 tccgttatcg tatttaccgt aagcataagc gcacttgata cgcctgcaca gattatatcg
      241 tttccgctct cagcgtaacc cgaatgacct ttgacggtaa aaccgataaa tttttcgcct
      301 tttacgacga atacagcctt tatcataaga cttatgcgtt tatagcttcg atcttgatct
      361 tgctgtaggg ctgtctgtga cccatcttgc gcttgctgct cttcttgggc ttgtatgtga
      421 atactgtaac cttcttagcc ttgccgttct tgatgagaga agcctttacg cttgcgccgt
      481 caacgtaggg agcacctact actgcgccgt cgtccttgcc aaccatgata accttgtcga
      541 ttgtgaactc gccttcaaca tcgagctttt caacgaagat ctcatcgcct tcttttacct
      601 gatactgttt tccgcctgtt tcgataattg cgtacatttt ttcaaacctt tcttgccctt
      661 aggcatcttg taaaattctc gctgcttgag gtgaaaccgc tatattaagc nnnnnnnnnn
      721 tctttatgac cctgcacatg cggcgatatt attatatcaa aaagaaagcg ctttgtcaag
      781 cgttttcttc cgatttttat tatacagagc cgatatacaa attatttgtg cgtcggctta
      841 ctcattaaac gttaaatctg aacagtacaa cgtcaccgtc acgcataaca taatccttgc
      901 cctcaagacg gacaagtccc ttttcctttg ccaccgccat gcttccgcaa gccataagat
      961 cgtcaaacga aacaatttcc gctctgataa agcccttttc aaagtcggtg tgtatctttc
     1021 ctgccgcctg aggagctttt gtaccgtttg tgatagtcca ggcgcgaacc tcctgaacac
     1081 ctgcggtaag atatgaaata agaccgagca ggctgtagcc ttgcttaatt atacggttaa
     1141 gaccggattc cttaagtccg agttcggaaa ggaacatttc cttatcttca gccgacatat
     1201 cggcaatttc agcttctatc tgtgcacaga tgggaagtac tacgctgttt tcagcctttg
     1261 ctatctcgca tacagcctta tactgctcgt ttttctcaat atcgcctgta aaatcggctt
     1321 ccgaaagatt tgcagcatag attacaggct tgtttgaaag caggggagat gcttttacca
     1381 tttcaagctc agagtctgta taagggtatg atcttgccga tttgccttct tcaaggtgag
     1441 cctgaagttt ttcaaggaac tctacctcag atgcgaggct cttgtcacct ttcatctgct
     1501 tcttttctct gtcgatacgg cgctgtacta tctcgatatc cgagaaaata agctcaaggt
     1561 taattgtttc aatatctctt gccgcgccta tcttgccgtc aacgtgaatt atgttgtcgt
     1621 cctcaaagca acgtacaacg tgaacaatag cgtcaacctc gcggatattg gataagaact
     1681 tgttgccgag tccctcgccc tttgatgctc cctttacaag tcccgcaata tcaacaaatt
     1741 caagtgttgc aggtgtgaac ttttccgggt tgtacatctt ggcaagtgcg tcaagacgtt
     1801 catcgggaac agatactatg ccgacattcg gctcaattgt gcagaaagga tagtttgcag
     1861 attctgctcc tgcatttgta agtgcgttga ataaggtgct tttgccgaca ttcggcagac
     1921 ctaccatacc aagcttcatt tatatattca acctttcttt aagcggtaac cgctgtgata
     1981 atccaataca gtataacact aattccgatt tttttcaagt agtaaacgat aatttgttga
     2041 cttgaagcgt aaagtctagt ataataagtt ctgcaaaggt ggtgattttt ttgcctgaca
     2101 tatataagca cggctacgac aacttcaaaa agataacggt gctgttcata tnnnnnnnnn
     2161 ntcatattgc agataattct cggtgttctg ccgtttgttc tgcttgttgt ctacatcggc
     2221 ttttcgctct attcaaatgc ggttctcggt tatgtattta tagcggtgat ggcagtaaca
     2281 gctgcagcga attttacgat aacaaaacga tataacgttt tgctgtcagg tttcaagggc
     2341 gaaaaatcgc ttatgaagac tgtaaaaaaa ctcggcggtg agtatactgt atttgcaaac
     2401 gttcctgtac ggtacaagaa aaacaggagc gagatcgact tgcttgtaat gtgcgacagg
     2461 tatattatta taatagaagt aaaaaatcac tcaggctata tttacggtaa ccataaggac
     2521 gaaacctgga ctcagagaaa gatctaccgt gacggtaaga ctaccgaaac gaatatgaca
     2581 agtccgatac gccagatgcg ccgtcagcgt gatatcataa agtcgatact gagagcaaac
     2641 gaccttgatc agtgggtcga tacggttctt tacttttctg cacctaccgt gcatctttat
     2701 cttgatctca gcgacagcga caatgtttgt tccagtgaaa aagagctgct tgaatttctt
     2761 cgcacctata aatcgcctaa gccgatagac agggataagc ttgaaaaaat aaaactgctt
     2821 tttaaagata tgtgttcaaa taaataattc tgaggggctg tgacacaacc cagaaaagga
     2881 aaggtgagtc gaagcgcaaa ggatagtgcg ctcggctcat tttttttgta tgtgaagaaa
     2941 atctgttttt attgcatttc aattatacag ttttagcaat taacattgat agcaggctac
     3001 cgatagttct attcgtcgga tagcacttct cgggttcgct atttcagtta ataacgttgc
     3061 tgctatgaac gagggaaaga accaaaggga ctagggagag ggacttgtgt atagtgtgat
     3121 aacatagttt acagaatgtg caaggacata gtttacatat
//
LOCUS       DAWAOG010000219         3150 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3970_length_3150_cov_2.483683, whole genome shotgun sequence.
ACCESSION   DAWAOG010000219 DAWAOG010000000
VERSION     DAWAOG010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3970_length_3150_cov_2.483683"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    289..362
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          670 a          726 c          709 g          971 t
ORIGIN      
        1 gctgtgatga aatttttgga tttcgtcaca gctccatttt tatgtccgtg cgaatacggc
       61 aaaatcaact gccgcagggt cgcatttttc tattgtatga tatatctcgg caagcgttga
      121 acctgtcgtg gttacatcgt caattacaag aacgtgtttg tctttaagag cattctcatc
      181 ggtaacggcg aaacaacctt taagatttac aagacgcttt tctctgccaa gggatttttg
      241 ctgggctgtc tttttgatct tggcaacagc ggtcactgtc ggtataccnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nngagtgtat caaagctttc cgttatcttt tgcataagcg tatatgccat aaagcttatc
      421 ttaccgccgt cacgatttcg ctttgcgcct aaaactatat gttctgaact gctattatat
      481 ctgcacacgc tgataagtct gccgccgcag aggacttctt ctttgctgca atattggaaa
      541 ttgtccgcac aatcgttgca gatataatcc attcttccta ttaccttatt gcacgcaggg
      601 caacggttgg gaaaaaagaa gtcgagaagt atccttttaa gctcgtactt attcatcatc
      661 gttttcatct atgaaaggat tacgtatctt tgtcggcttt attataccgt caagcaggcg
      721 cttggcggtg ctcctgctta ccgcgctgtc cgaacgtctg cgtctgtcag aacccggtat
      781 atcctcgctg tcggtaagat cgtctatgct cggcgcatca agctttatga ccggtgctgt
      841 atggctgtgt tgcccgccat ttggctttgc cggctttatt gcatcggttt tttctgcttt
      901 ttcggatatt tccgggacag ctgttgacgg aagtatgtca aagtcatccg aagtcaggcg
      961 ttcgccgtca ctgcgtattt cggggtacag cgctgtaagg ccatcagact gctctgactg
     1021 cgttgtttcg actgtatgtc tgtacatatt tgcggcatag ctgctcttcg gagtagctgt
     1081 ttttctgatg ttgtagtccg cttttgcaaa ttcacccgca aagcctgtat ctacagccgc
     1141 ttcacttttc ctgtcgggag tgggcttatc atgtgtgttg ctgtttgcgt tgcttgtatc
     1201 tgtatgcggc gtgtcggctt ccggctcgcc aaaagagcca aagctgaaaa tgtcatcaag
     1261 aggcttgaaa tcgtggtcgg ctgtgacctt ttcaagcttt gatatttctt cttcaaatgc
     1321 accgtcaaac ggattatcaa atacggtcac gtcaagattt tcggtttctt ccgctttcat
     1381 aacgtgcacc ggttgtttcg gcgtggtatc gggtatttgc tccggcggct gagccgatat
     1441 tctttcctgc tcgcgttcga gcagttcgcg ctcctgttcc tgcttcctgc ggcgcttctc
     1501 ctgctcgtaa agcacactgc ggtcaacacg ttcaaatacg aacggtttta cttcaggctg
     1561 tttcgcagca ttgctctgtg tattatcagt tgcctgctca ggctgttttt ctgcgacagg
     1621 ctcagggggt tttacaggtg caaaatgcag gtcgtgagag taattctcaa actcgcggaa
     1681 ctccgcaaca ctcagcgtat tatgcagtat gctttccttt atcgccataa agctctgata
     1741 ccattctgca aaatcacgct gtgtcaggtc aacgtttatg cgctttgaca tatatgtgta
     1801 cagctctttt gtcatatcct caagccgtct tatctcgtct gccgttctcg gcggggtagc
     1861 agtgctcagt tcaagacagg tacgtccgcc gggctgtgcc ttatcgcagt aatcgtaatc
     1921 ataatccttg tcacgcagat agtattcccc acaacgtgca cagcgctgta atattcctcc
     1981 gctttcaagc agaagatcta tttcaaggtc gatgactgct tttagactgt cggaaacgta
     2041 aacctttttc ggcagagaac gcattgtcgt tattatggta ttcagtactg agtcgggctt
     2101 ttcgggaata taatttttga tcttagcaaa cacatccata gcttcgctgt cggaatgcat
     2161 tgatgagaaa ggacggtgta cagcatcgtc atagtcgatc tcaggcagtg cagagcctgc
     2221 tatagccttt gagatatttg aggttataaa cggcgctgac ggcagtctgc cgaccgtgta
     2281 tacctttgcg gctgtgaagc actccgaagg gtagcccatc tcgtttgcgg ctacactgaa
     2341 agaaaggtca aaataatcgc gcttggcgta tgcctgttca agggtcactt tttcgccgaa
     2401 tacaaaatct attttacgct ttgcgtattc ctgaagcctg agcaggctca cccaacggtt
     2461 caccgccttg ttgtgacgat attcggtcag ccttgtgaat ataagctttt caaagggatt
     2521 tttagcgttt atcagttcgt tccacagtga tgatatgcca agtccttttt ctctgcaaat
     2581 gtattcgatg aaaagatcct gaacgacctt ttcatatccg ctgtggatat tcgcttctac
     2641 atatttgtgg cacgctccga gagaattacg cagtgcgact atatcttcaa gacggtattc
     2701 gccgcttgtc atacaacgct tgcagtagtc cgtacagcgt ttgataaagc ccgaaaagtc
     2761 gtaatctgca aacttcagga cagactgcgg atagttaagc gcatatattt cactcgaatt
     2821 tatgcctgtc aggtttactg tacgtcttat gctcatatcc gcatatcctt tcgctgctta
     2881 cgatcaataa tcttcttctg cggctgtatc gtctgtaacc gcgtcaaagt tgaactcgcg
     2941 ttttcttccg tctacataga gagtatgctt gtctttccat gttacatcgg cgggcttgtt
     3001 gtagtcagat gtgattatgt gcagtccgtt cattgcgttt ctgcattcaa ggaacggaag
     3061 gctgatatct ccgtctgtct gttcaagata gaaggatacg gaccggcttg cgtcattctc
     3121 gggatcgaca tagagcacta tcctgtactg
//
LOCUS       DAWAOG010000220         3148 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_3974_length_3148_cov_2.449079, whole genome shotgun sequence.
ACCESSION   DAWAOG010000220 DAWAOG010000000
VERSION     DAWAOG010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3974_length_3148_cov_2.449079"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2638..2647
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          733 a          788 c          698 g          919 t
ORIGIN      
        1 gaatacacgg tacaaaacta tatcaagcag taccagaact ataactaccg cactgaagct
       61 tttgagaaac cagcggttag ctatcgttgt gcgtcttgcc aaagccttta cctccgttta
      121 tttatccgtt gaactcgcct gcggcggcaa gctcgtctac acaccatttg tagccgtagc
      181 cccatatggt ggttatgtat ctcggatttg acgggtcttc ctctatcttc attctgagcc
      241 gtcttatatt gacatcgact atcttgtcat cgccgtaata gtcctcgccc catatgcggg
      301 tgagtatgct cttgcggtca agcgcagagc cgctgttttc cataaagtac tcaagcagct
      361 gatattctac ctgcgttatt tcaattccaa taccgttttt tgtgaaaata cggctttttg
      421 tgtttattga gaacggaccg gaaataagcg tcctgccttc gtcctcgggc ggtcttgcca
      481 cgctcaggct tactctgcgg taaacagcat cgacccttgc gacaagctcc gatggcgaga
      541 acggcttggt tacatagtcg tctgcgccct gtgtaagaca gcgtatcttg tctacctcct
      601 gactctttgc ggagagcata atgataccca tagtatcgct cttctgcctt agccagcggc
      661 aaagctcact accgtccatg ccgggcatca ttatatcaag cagagcaaca tcgataagcc
      721 cgccgtagtg tacatatgct tccatagcct gtgcggcgtt gctgacatca acaacatcat
      781 agccgctgcg ctttaagttt attacgacaa aatcccttat cgcgtcttca tcttcacaaa
      841 cgagtattct tttcatatca ttacctgcct ttcaccgcat tgttccgacc gggttttccc
      901 gctcacggcg cagtattgct tgtgtatcct tccggacgta ccttgagagc cgcggctatt
      961 tcgtcttcgg taagcgacag cccctcgtac ataatgcttt tgtaatatat cgtcttttcg
     1021 ctgtcgtccg agaacaatct gtatccgtca cgcaatgccg actgcacatc gggttcgctt
     1081 tgagaagagg tgtcattttt aagcacgact tttattgtga gcagtgcgta ttcgttcttt
     1141 tttatatcgc cttcatactt ccagaaagaa accgtgttgt catctgcgtt gacagccgcc
     1201 gttaccattc ctacccatct tccggggaag gtaagcatat agttcctttt cgggtcgatg
     1261 aatgtataag cagtaagctg tattttatcg cagttctgtc tgtaccagcg cactgcgcat
     1321 atctgctcgg gataagagag attttcatat cccggcagcg gtgatgtcgc cgggatatct
     1381 attatcccgt cgccgtctat atcctgtgaa cgcagtttag gtgtatagct gtttgtgcgg
     1441 cgtgaatagt tcgttttcgc tccgtcccgc actatcgtgt cagctgttgt gaatgcgttt
     1501 ccgttataat acagtacgct tgtaccgtag cagttttccg aaatcagata atcaacggct
     1561 atgcacggca tttccttgcc gagtataaga cagcttgcag acattatcct gtcaaaatcg
     1621 gtaactccgt ttccaaatga cagtacaggg tacatcatac cgagagtttt gccgtccgta
     1681 cagccgtaga cggtcatatt gccgcttttc tttgcgccgt cgcggttgaa gcaggcgaga
     1741 tattctccgc tttcggtcag attatccagc atcatatatg acgcatatcc tatagttcca
     1801 agctgtgtcg caacaccgtt ttcgtagtcg atcactgaca ttatcttttc ggaagaaccg
     1861 agaacagagt agcttataag cagcttttcc ttatctgagc cgagttttga aaaggcaacg
     1921 ctttcgacct cagtgcctgc cgccggaagc tcataagtcg ctttccagtt accgttttca
     1981 tccttatcaa ggaagctcac acgcaggttt ccgactgcgt ttccgtcctg tgcgtccggt
     2041 tcttttgcgg cttcgctttt gctttgctca taaaagacta tagcctcatc ggtaggttcg
     2101 ccgtctatat tgtggataac gaatgcactg cggtattctc cggtatgggg ataacgcaaa
     2161 tcaaccctgc ccgcatttgc ggtcagggcg ttgtatattt cgttctgttc gtctgtaagc
     2221 cttggagaag ctaaaagctc gccggcagaa aaagagcccg agcaaccgca gagcacagca
     2281 ataagacaca ccgatgccat aagccctgct gtcattctga gtattttatt aaggcgtagt
     2341 atagtgatgt ttttcatttt tactcctcgg acattttatt gtatgtcctg tgtatcgctt
     2401 tcagaaacgg ttttttccgt tgcggtaatg ttctgcccgg caacgggagc attttccgcc
     2461 tgtgcagaaa ctcttttctt tgagaattgc acgatacctg caagcacagc aggtacaaat
     2521 gcggttatta taagttcaag gtcgggcgtg tcgataagct gtgcccattt ttcggcgttt
     2581 gttaccgcaa gcgcaaaaca gcttataaga tacgcggcag aacacgctcc gctgaaannn
     2641 nnnnnnnagt atagtcagta tatagaacat catcagcata agcttctgcg gcataccggt
     2701 tattatcgga ctgccgagat agaacctgac agctacaata agatagttga taattataaa
     2761 caacgcgctt atgcactgtg cggggactat ctttgacgat gacattatcg tcatacctcc
     2821 ggcgatatat gctacacagc ctgcgagaga tgttatcgtg caaattccga ccgtcccgga
     2881 agaaaaatcg tttataacag gcagtatcaa tgccgcaccc gcgagtatca ttacaacacc
     2941 aacggcatta cagctttttt tggacagctc atctgctgtt tttgctcccg agcctttaat
     3001 gtcaaggaaa gcaagaatgc ttattgccac cgccgctata acgagcagta tgttgtatac
     3061 ggtgttaaga gcctcgcttc ctgccgcata aagattggac ggatattctg tacctgtatt
     3121 gagctggatt atcctcagca cagtcagt
//
LOCUS       DAWAOG010000221         3126 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4009_length_3126_cov_2.234126, whole genome shotgun sequence.
ACCESSION   DAWAOG010000221 DAWAOG010000000
VERSION     DAWAOG010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4009_length_3126_cov_2.234126"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1125..1168
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          865 a          533 c         1062 g          622 t
ORIGIN      
        1 cgctcgatca gagaaaggaa ggacgcaggg agacaattta tatgctgtaa gcagaaagga
       61 gaaatatgaa agagaagata ggcagggcac aggtgcttgc cgcggcggcg gtgctcagaa
      121 agtacaagga aggaaaggcg gtgctcgaca agaggatagt gtcaaatgag cagtggtgga
      181 aaatgcggca ctgggcggag atagggtatg agcctgacga tacaagtcct cagcctgcat
      241 cggcgtggct gttcaattcg atagctaaca agcacgcgga cgctatggat aatatccccg
      301 agccgtctgt acttcccaga gagcagggag atgaggagtg tgcaaggcag ctgtcgctgc
      361 ttctgccggc ggtgcttgaa cggtgcggat ttgagaagct gtacagtgac ggctggtggt
      421 acaagctgaa gaacggtacg gcgtgtcagg cggtagtgtg ggatcctgcg cttgaggacg
      481 gcaggggaga tattgctata agaaatgttg atatactgaa tctgttctgg caaccgggga
      541 taaaggagct tgaagagagc gcaaacctgt tctatgtatc gcttgtggac aaggagcagc
      601 tgtgcgaaat gtatcccggg cttaaagacg gtatatcgga ggaaacggtg aatgtcgaaa
      661 aataccgaac agacgacagg actgacgaca gcggcaaggc tgaggtcgtt gactggtact
      721 ataagaaaac ggtcggcggg aagaaaattc tgcactattg caggttctgt ggggacaagg
      781 tgctgtattc gagcgaggat gacgagagct gtaaggacgg gttctacagc cacggaagat
      841 atccgttcgt tatagatccg ctgtttacgc aggagggcac accgtgcgga tttggctata
      901 tagacgttat gcgggacgcg cagacgtaca tagacaagct gtcgcaggtg gtgctcgagc
      961 atacggtgca gatgagcaga aagagatact tcataaggca gaacagcgcg gtgaacgagg
     1021 ctgaatttgc cgaccgcagg aatatgtttg tgcacgttgc tggcagtctg acaaatgacg
     1081 atataagaga gataaagaca gagccgcttg acagcgcggt gatgnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gcaacaggga cttttctcag gggtcgacct
     1201 cgggaggcgt tacggcggca agtgcgatag cggcgctcca ggaggcggga agtaagctgt
     1261 cgagggatat gataaaggga acgtattttg cgtttcagca ggtgtgctat cttatcatag
     1321 agcttatcag acagttctat gacacgccta gaagcttcag gataacaggc ggttatgcgg
     1381 cgtttgacaa ctcggagata aagagccgcc gggaagagct gttcggggtg gagcttggag
     1441 aaaagaagcc ggtgtttgat attgtatgca cagcggctaa gaaatcgccg ttttcaaagg
     1501 cggcacagaa tgagctggca aagcagctgt tccagctggg atttttcaac ccggatatgg
     1561 cggtgcaggc actggggtgt cttgaaatga tggattttga gggaaaggag agcgttgaac
     1621 gcactataag agagggtgcg ggtatgacgc agaaaaatat tatatgacaa gggtaaggat
     1681 agacaggtca aggctcggca gggatattta tatcacggga cattgccaaa acgaggggga
     1741 cagctcgagg gaggcatcgc ttgtatgtgc ggcgcttaca acgcttgcgc agactatagc
     1801 gcagaatgta tatgacagcg aggatacagg cgacacggat atcatagatg tgacgctgaa
     1861 aagcggtcag gcgataatca gctatgttac ggacgacgag gggctcaata cggcggttga
     1921 cggaataagc aagggatttt ttatgcttgc ggaaaactat cccgagtatg tggattgcta
     1981 tggtaaagag gggtgagtat ggtggaaaac gaagctgtga atgccgaaaa tgcggcggag
     2041 gaaaagcaga cagaaaatac ggatatcggc acggaaggtg atgctgatgc aaatggcggc
     2101 gaaaagcctg cacaggataa tgcaggcggt aacacctctg cgggatatcc cgatgaggtc
     2161 ggaggtgaag agttcaggca ggcggtgctg agaatgaagc aggcgcgtgc ggggaaagcc
     2221 gaggggatag taaggcttgc ggcaaggctg tgcggggcgg acgaaaacga ccttgacggc
     2281 gtagaaagtg ctgtgataga gcggcagtcc gaatgggaac agaggaacga tatgcggtac
     2341 aggcttgcca aatggcgcgc ggagggtgag gctgtaaagg agctgtatcc tgaattcgac
     2401 cttgtcgggg agatgaaaaa cagggatttc ttctcgctgt gctataaagg aatagggctt
     2461 gcggacgctt atcttattgt gcacaaggac gaaattatcc gctcggctat ggagtatgcc
     2521 gcgtctgagc ttatgaaaag cggggcgatg agagcggacg gtaagagaat gagagagagc
     2581 atgctgtcgg tgagcaatgt acccgacaac agtaagaagc tgccaaaaag tgagagaaag
     2641 gagctgataa gaaggacgga gagaggggag agggtggtgc tttgataaat gaaaaatgaa
     2701 aaatgaaaaa tgaagaattg gggtggtgcg gggcgagggg tagtgcgggg ttggcggcga
     2761 tggtggggtt ggcggcgatg agtgagaatg aatgatgaaa aatgaataat gaagaatgaa
     2821 gaattgtggt tgcgtctgcg gtcgttagtg tcgctgcgtc cgtgcagcga cttagtgcga
     2881 ccgctattaa agcatccgcg aatttcgatt tttgatagtg cggggcgagg gatagtgcgg
     2941 ggttggcggt gtttgtgaaa gtggagaaaa tcggggtaac gggcggggca agccgccgcc
     3001 cctacgggaa ttgggtagtg tacgttgctt ttgcagtgat ggcagaaatg gaaatgtagg
     3061 cagtggataa aaaaggatag tacaaagtga tagtgaagta ggaagtttga aaatagtgaa
     3121 aggttg
//
LOCUS       DAWAOG010000222         3112 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4038_length_3112_cov_2.055610, whole genome shotgun sequence.
ACCESSION   DAWAOG010000222 DAWAOG010000000
VERSION     DAWAOG010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4038_length_3112_cov_2.055610"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2084..2093
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          869 a          616 c          723 g          894 t
ORIGIN      
        1 gttcggtagc gccctgagca agtgactgag cgcctgcgga tacctgctct gagccgcttg
       61 atacctgatc gcctgcgatg cttatctggc ttaaagtatc attgagcttt ttgttgatat
      121 ctcttacaga ttcaataagc accttgtaat cgccacggta tgtattatcg gcatctttgc
      181 tgtgtacatc aaagttgcct tgagccatat tgctcaggat attgccgata tcacttatca
      241 tagcgttctg ttcatttaca accttagcgg ctgacttcgc aagtattttt acctcatcgt
      301 gcgatttaac cataggcaca ggacttgtga ggtcaccttc tgagagcttt tcgatacgct
      361 ctgcacaaag tcttataggc ttgccgatct ttcggctgat agctcctgcg ataataccgc
      421 cgactacagc agatgcggca cagattattg caagtataat aaaaccttta aagcagtgaa
      481 ccatatagtc atcgctgtgt gctgttacgg caatagacca accgtttgta tctggtatgg
      541 gggcatagcc gacaaattta gttatgccat ctgatgtgta ttgtgcgaag cctttttcac
      601 cggcctccat tttttcatgc gcctgggcaa gagcggtcca gctgggatct tcctcggctt
      661 tcttctgtat attttcgccg tcctttacaa gttgcatatc aacgtcggca attgtgttac
      721 cgttcttgtc tatgatatac gcggcgccgt tttcgtttat cttaagtgtg cggacgatgt
      781 cattgagaaa ttcttcgttc ggtacaaaat atatacagcc tacaacttta gagttgagct
      841 caccgttttc ccacagggga gcggcaatta taacgctcag cttgccgttt gatctgccta
      901 taataggttc tgtgatcgtg gtcctgcctt ccatagcggc tttgaaataa tcacggtcat
      961 cataattaac accgtttgat ccgtggcctt catagtcgat agacgcacct ctgtcaagac
     1021 cgaagcttgc aacgatcgta tcgatctccc tttgtttctg tgaatttgta tagatcacag
     1081 atgatagttt tggattgcag cctgcctgaa ttgctatatt ctgtatagcc ttcagctccc
     1141 atgaaacttt gtcggcggca accgttgccg tttcggtcag ggtttgttct gtaagtgatt
     1201 ttgtcgtggt gtaaattatc gctaccgatg cgaatgtcgc caccaacaac gatattacta
     1261 ccagcattac agtgaagaat gtcatttttt tgcttataga tttcataagt atctgcctcg
     1321 tttccgtacc ctcgttaaag atacatattt aatacatacc tcaattatat cactttgtga
     1381 gaaaaattgc aatattgtac tatttatttc ggcaatagta acaaaatttc gacgtagatt
     1441 tattgcgtca cgctgaaatt acagtgtgtt tcagaaaaat ataaataaat tcggtttttt
     1501 tgtagacagt ttatgcttat ggggtgtatt atgtatgtag atgtacattt tcggctataa
     1561 aaagaaaagg gggaagggtt ctgatgtcgg attattcttc tgaaaacata agagaaatat
     1621 atgagcggca ttttgtcacc gtgtacagag tgtgctttat gtacctcaga aatgtgcacg
     1681 atacagaaga cgcggtacac aatacttttt tgaagcttat tgaaaaacga aagagctttg
     1741 agagcacaga gcatgaaaaa gcatggctta tacgcgtatc gcagaatgta tgcaagaata
     1801 tgctgagggc aaacaacagg aggcgaggtt cgctggcaga ggacattgcg gtgaatgaca
     1861 ggtctgcggt cagcgatgtg atggttgaac tgtcggagct tccggatgag cttaagatac
     1921 ccgtgtatct gttctattgc gatggctata acagtaaaga gataggcagt atgcttcata
     1981 tcagcgcatc tgcggtgcgt tcgcgcctac agaaagccag agagcggtta aaagagcatt
     2041 taagctcgga aaggagtata gtatgagtat cagaaatgaa tttnnnnnnn nnngtaatgc
     2101 gctgcacatc ggttgcggcg gcaacggttg tggttgtcgg aactacagca gtcggtattc
     2161 attttgcaac aagaggcaat ggtatagatt acaccgcgag cggtagttca tcgggtacgg
     2221 tcagcactgc cgaacttgcg acaagcggag cggacgacag taatggcggt gtttcaaacg
     2281 cagaaaatca gtcaaatggt aatgacagct tgtctgataa ctcaagtccg gaaagcaatg
     2341 cggactcatc tgttgataat gtaacagatc cggaaataac aacggacaat aacggaaatt
     2401 atattatcaa caaggcgctt tataacaaaa taatggacga taagctttcc gatgatgaat
     2461 ttgcaaagct ggttgagtat gtgcataaag gagattatat tataaacgaa gatctgaacg
     2521 agcttataaa agcaagagcc gaatacaata aaacgataca gcagacattc agaagattta
     2581 tatcgtcagg cggatacgag aatgacgatg aatggcatat aacacctgct acacccggca
     2641 actgccacag catgaaagaa tggtatgata ccgcttgcgc gctgtatgcc gatatgccgg
     2701 attatgatac attccttagc agatacagtg aatatttcaa atttgacggg cagggaaatt
     2761 tctactatgc gacaggcaga cccgatgagc agttgcttgg cggaagaatg ccgtttaatg
     2821 caccgtctta caacactttt gatgtatcac cacgctgttt tacagtgaat gagagtacaa
     2881 gcggcggaac gggactcagt atcggcgttc tgtattttga cagtgtaggt acagatggca
     2941 cagcttacag cgtaaacgtt gcttattttg atgtaaagaa aacaccggaa ggatacagat
     3001 ttatgagaaa cgaaaacagt taaagtatag tatgaaaggc agagccgcgg ctctgccttt
     3061 caggttgtag aaaaagtctg tttttttaga aatgtagctt attctcaaac cc
//
LOCUS       DAWAOG010000223         3090 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4073_length_3090_cov_2.540692, whole genome shotgun sequence.
ACCESSION   DAWAOG010000223 DAWAOG010000000
VERSION     DAWAOG010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4073_length_3090_cov_2.540692"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    869..973
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2401..2410
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          908 a          684 c          599 g          784 t
ORIGIN      
        1 agggtattct ttccgctgaa tttgacgtca agataaacga cagcgatata ggaaagctcg
       61 caggctcgct caatactgca aagtggtatc tcaaaaaaat ggtcgatgag cttacatatc
      121 tgctgactca gatgtcatac ggcaacatca gctttaccat taactatgat tacaagggcg
      181 aatttactcc cataaagtcg gcatttgagc agatacttgt aaatcttaat gactcgtttt
      241 caagaataca gaaggcagga actatgctat acgatgcctc cgagcaggta tcaagcggtt
      301 cacaggcact tgcacagggc acaagtgagc aggaagcctc tatccgtgaa ctttcagact
      361 atgtaagcga tatttcaggc aaagtaaaca agaatgccga aaacgcaaag aatgtaagcg
      421 aaatttcaga tacggcgacc aatgacctta aggaaggcaa cgcagaaatg cagaaaatgc
      481 ttgccgctat gaagattata gacgaaaaat cagccgagat cgagaagatc atcaacacta
      541 tcgacaacat cgcattccag acaaatatac ttgcccttaa tgccgctgta gaggccgcaa
      601 gagcaggtga agccggcaag ggattcgccg ttgttgcaga cgaggtaaga aaccttgcga
      661 gcaagtccgc tgaagccgca cagacaacaa gcgagcttat cggcagcact atagaggctg
      721 tttcaaacgg cacggctatc gcagattcaa ccgcacagac tatcgaaagc gttatggaac
      781 gtttcagcaa tgcaaacgca cttatcaacg aaatatcgga cggctcggca cagcaggcaa
      841 ccatggttga ccaagtgctt tcaagcgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnngaagaat aacagaaata gcaaacaaaa gcaggctgaa aatgcctgct
     1021 ttgtttttac ggagaatttt atgactacac aggaatttga acacaacgga atgcactgca
     1081 agaaatggct ttccgataac gaaaaagcta agatacaaat agttcacgga ctcggcgaaa
     1141 tgtcggaata ctatgagcag ttcgcagaat atattaccgc taaaggagtt tcggtgtatc
     1201 tttgcgaata ccgtgaacac ggaaggacgg cactgcctgc aaacatagac aatattgttc
     1261 agactgccgc aaaggaatgc ggtgattttt catcatacat tcacttggaa agtgatacgc
     1321 cgctgtttct gctcggtcac agccttggcg cacagatggc tcaatatgtt atttgtcact
     1381 gcaatagctc gctttacagc ggagttatac ttacgggctg tccgtatata catgatacga
     1441 aagcgctttt gtcggacata gaagcggaaa tttccgaaaa aggcgcagac gcgccgagca
     1501 ccgatgtttt tcttaaacta ttcggtaaag ttgccgagcc ttttccggaa aaatgcacag
     1561 tgtcatgggt cacctcagac cttgaacgcg cggaatacta cgaaacactt ccctatacaa
     1621 ataagatgta cagctgtcga ttttaccgca gcttaatcgc tctgtcaaaa gaagtacagc
     1681 aacaagacta cctctccgaa ataaatgtta aaccacccgt acttatgata agcggcacca
     1741 tggatacagt cggagataga ggcgaatacg caaaatcaaa agccgctatg cttaaatcga
     1801 tcggctttga cgtaacgctt cgattattcg acggtatgcg ccacagcgta ttgcaggaga
     1861 aaaatcgcga aaatgtctac cgacttattg cagaatttgt tttacagcaa tacatgatga
     1921 tttgataggc atatataatg gactgcgaca caattcctcc tcgtccctta ggttgtctcc
     1981 ctcacacata gcagcaacgt tatcgtacag aatagcgaac ccgagaagtg ctacccgcaa
     2041 aaggcactat cggtagcctg ctatcattgt taattactaa aacgttataa tagaagtgta
     2101 ctaaagtcag actttctcca cataccaaaa aagtgagccg tgcgcactat tttgcgcttc
     2161 gactcacttt tttcgggttg tgtcacaacc ctcatcttta tacatcattg cagctttaca
     2221 tcgacccgga atatcggtat gaccgccgtt atacctttcc cgtccttttc ccctcttcat
     2281 aaacgaaata gcgcttcttt atctgtgctt tgctcttact tctgataagt acgctttcac
     2341 cgttatccag tatgaattgc tcgtcaaccg cggtgatatg atccatattt acaatatagc
     2401 nnnnnnnnnn cttaaaaaac gtgactcctc acccgacagc ctttcttcta tttcatcaag
     2461 ccgcatatac aatgaatatt ccccttctgc ggtatgcagt gtacagcgac tgttacagct
     2521 ttctatatat attatatcac tgtacggaac attcacataa gtgttgcgca cttttactct
     2581 cataaaatct gtgtcatact tttcaagaac tctgtctagt acgttttcaa gcctcggcat
     2641 attatacggc tttaatatgt atccgtttgc ctttacgctg tagctttcta tagcgtactc
     2701 gtctgtgatc gttgaaaata tcaacagatt ttcatatccg acgtttctga gttgctatat
     2761 caatgccaag ctcctgcccg acatagatat ccataaatac gatatcaaaa ctcttgccgt
     2821 cgattatatc gcgtagcagt ccgctaccgt tctcataaag cacaacggta caagataaat
     2881 gatgcttttt gcaatattcg attatcaatg tctttgacag cagtgcgtct attctgctgt
     2941 catcacatat tgctatcaac atttgtaact gttcctttgc cgtatgatac aatcacgcaa
     3001 gctgtatcaa aaatcgaaat gtaataatga gaaaacatcc gtatcctcag ataatattat
     3061 aacttaaatc aagtcggtgc aagccgacac
//
LOCUS       DAWAOG010000224         3073 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4104_length_3073_cov_1.938370, whole genome shotgun sequence.
ACCESSION   DAWAOG010000224 DAWAOG010000000
VERSION     DAWAOG010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4104_length_3073_cov_1.938370"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1224..1233
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2540..2549
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          793 a          696 c          678 g          886 t
ORIGIN      
        1 gtgatatgct ttatatagct gtcagccacc ataaacaggc tgtagaacgc agttgtattg
       61 tagtcggacc tcggcatcag aggatactca aactgaaatt catagtcctc gttatagtac
      121 tcggtagtat actcgttttt cggaacatag taggtgaatg tttccccttc gtacataagt
      181 ccttcatact gagttgccgc atagaccgag ccaaggcagt cctcttcata cttgtcatag
      241 tcggacagct ttgcaaatct tacgcctgcc gtttttgcaa agctctgagc cgcatagtac
      301 gctccgagct gctgccagtg gcagtcgcgc aggaaataca ggttttcttt acgatgcgga
      361 aggagcgctt tcatagtatc tatgaccttg atgtccttgc taagcatatt tgagatatat
      421 tcagagtcgc taagctcggt atactttgac ttttcaagct cggcgggggt tataaatgta
      481 ttctgggtaa gcgccagcat actgtataca tcggtatccg ggagcagttt tttcagtgta
      541 ttgagggtat gcgcatagtc ctctgcattg tcacggtttc cgttatagag ctgtgcgccg
      601 tatattgtgt cgccgctctg atatacaagc tgtgagccct cttcataaag cacctgtgca
      661 tccgggtcag agggcgctat atatacgttt tcgggctttg gagcattact gctgttatcc
      721 gactttgagc ttgtttcggc ttttgagctg tcatttgacg atgtttcggc tttgctctca
      781 ggtgctgagt tgctgctctc gggagcattg cttgaggtat cggacggcgc aggtgtaact
      841 atagctatgg gcttggttga cgcagttgtg acctcggtct gtgacgctcc gctgttcacc
      901 tgcgaaacag tgccctttat ctctgcaccg tctacagtaa agccgaacag tgagcgaaat
      961 cctgcaccga ttgccttgaa tgtatctctg aacggtacgg tgtcgttaaa gaaatagctg
     1021 atatcgtttg tgtagtcacc gctcagatat gcgtcccatg tgaatttcgg gaatgtggca
     1081 aggttacgct tttcagattc ggatacagtc ggacgcggaa gcaccagaag cccgacggca
     1141 acaaccgcaa agaatgcagt gcatacgccg atatttatct ttacgccaag ctttttcttg
     1201 cgctcgtctg cgctcatagg cttnnnnnnn nnngctttat cacgcttgtt ctttttcggt
     1261 tcgcttgttt tctgagcttc catcttgcgg cggtatattt cgttaagctc ttcaagctcc
     1321 ttgtcatcta tatgatctgc caatttactc actcctttct cagaactgcc aatagataaa
     1381 cgggttgttt gtggcattta caagcagtat actgctcagt gcgaataccg caacattaag
     1441 caccgtctga cctacagagg ttgcaagata ataattcata ctctcaagct tcttcttgag
     1501 tgcgggaaca acaggcatac acagcgctac gcttacaata aaaagccacg catttgctgt
     1561 catattctgt gtagatatct tatctacaaa tgagttgccg ttaagtccga caaggtttcc
     1621 gaggaatgtg ccgagcttgc caagatcggt aaagtagaat ataccgaagc ccaccacgac
     1681 tactatcgta ttatatatgt gcgcaagcgc ctttggcatc tttctcatat tcttgttgcc
     1741 tattgtgcgc tctatcagaa tgaacagtcc gaagtaaaga ccccagatta tatagttcca
     1801 gtttgcgccg tgccagaagc ctgtgcagaa ccatacgaga aagaggttaa gatactttct
     1861 tctcttgccg aatatcggaa cgtacagcag atagtcgcgg aagaatgtac caagcgagat
     1921 gtgccacctc tgccagaatt cggtgatatt cttgcttaca aaaggatagc ggaagttctc
     1981 gtcaaagtgg aagccgaata tgcggccaag acctatcgct atatccgaat aacccgagaa
     2041 gtcaaagtag acctgcatag cataaactat tacggtgtac catgttccga ctacggataa
     2101 cgatgcaagg tcgccgttct tgaagaacgt atcaactata gtgctgaggt tattggcaag
     2161 gatcaccttc ttgccaagac ccatacatat tcttgtgata ccctcgctga tatcctcttt
     2221 ggttatcact ctgttatcta tctcatcttc aattacagca taacggacga taggacctgc
     2281 aacgagctga gggaacattg agatgtacat aagcagcttg taccaacttt tctgaacctt
     2341 tatcttgccc cagtagcagt caaggatata ggagattatc tggaatgtga agaagcttat
     2401 gcctatcggc atttctatat gcggcacggg taggtcgacc gggcttatcg cattgaaatt
     2461 ttcaacaaag aagcctgtat atttgaagaa catcagaagt cctatatcaa atataagagc
     2521 accggcagtt accgcggtan nnnnnnnnnt agcttttttt gtatcacgga acttatctat
     2581 cagcagtccc gcaacatagt ttaccgtaac gcttccgaca agcaggaaaa cccagaccgg
     2641 ctcaccccat gcatagaata tcatagagaa tacgataagc acccagttgc ggtatactgt
     2701 cttacggcag ataaaatatg ctataaagca cagtgccata aagaaataca gaaaaaataa
     2761 atcggcgaat atcaactgta tcgtcctttc ttttgaagct tgttttaata tcttttatgt
     2821 gttgtctgcc cgttttgtga gcaaacccat tcagtatata attatactat atcaaacgcg
     2881 gtatgtcaat ttcaatcaac cccgacttta tccgaaaaat tgctgtttcg gacgctgttt
     2941 attgtgacaa ataaccactc ggcggcggag cagaaaaatt gcccccgaaa atcgggggca
     3001 accgttaaat tcaattattc gtttgtttca ttgatatcgc tgttatcgtt atctccgata
     3061 acctgagcgg cat
//
LOCUS       DAWAOG010000225         3063 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4121_length_3063_cov_1.870678, whole genome shotgun sequence.
ACCESSION   DAWAOG010000225 DAWAOG010000000
VERSION     DAWAOG010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4121_length_3063_cov_1.870678"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1772..1850
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2494..2503
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          888 a          680 c          754 g          652 t
ORIGIN      
        1 acgtagggca tacgtcagtt gccggggata tgttggtatc ctgcaaagcg cacgaccgca
       61 aatcgtgaaa tcaaggtggc accgcggata atagattatt cgtccttgac agaaaagcta
      121 tttttctgtc aaggactttt ttgtttttgg cagacagaaa acggaggcaa agaaaatgga
      181 gtttttacca acccttgaaa aagcaaagga aatagcggcg cacaacagat acaaggtcat
      241 gccggtaagc tgtgaaattc tcgccgatat atgtacgccg atagaggcga tgaggatact
      301 taaaaacgta tcgactcatt gctatatgct tgaatcggct caggcaaacg aaaaatgggg
      361 tcgctacacc tttatgggct atgatccgaa aatggagatc acttgtattg acggcgagct
      421 taaagcaggc aacataacgg tacacaccga cgatccgtcc gcatacttaa ggcagatact
      481 cgcagaacac aaaagcccta agttcgatta cctaccctcc ttcacaggcg gactggtcgg
      541 atatttcgct tatgattttc tcggctacag cgagccgacc gtaagaataa agacaataga
      601 caccgaaagg ttcaaggata tcgacctaat gctgttcgac aaggtcatcg cgttcgataa
      661 cgtaaagcag aagataacac tcataatcaa tattccgctt gcagatgtcg aggtcggcta
      721 taacagggcg ataatcgagc ttaaagctat ggcagagctt ctcgctcacg gtgaaaagaa
      781 gtcagaaccc gcaggaaagc tcctcggtga tgtcactccc ttatttgaca aggcgcgcta
      841 ttgcggtatg gtcgaaaagg caaagcatta tataaaagag ggcgacatat ttcagatagt
      901 gctttcaaac aggctttccg cgccgtttga gggcagtctg ctgaatacat accgacagct
      961 cagaacgata aatccctcac cgtatatgtt ctatttctcg ggtactgatg tagaggtcgc
     1021 gggtgcatct cccgaaacgc tcgtaaagct cgaaaacggc gttcttcaca cattcccgct
     1081 tgcgggaaca cgtcccagag gtaagacaga cgaagaggac aaggctcttg aagaggatct
     1141 tctgcaggac aaaaaggagc ttgccgaaca caatatgctt gtagaccttg gcagaaacga
     1201 tctcggcaga gtaagcaagt tcggcacggt acaggtcgaa aagctccact cgatagagcg
     1261 atactcgcac gttatgcata taggctcaac ggtaagaggt gagataaggg acgatcttga
     1321 cgcacttgat gctatctcgg cggtacttcc cgcaggtacg ctgtcgggcg cgccgaagat
     1381 aagagcttgc cagctcatcg gagagcttga gaacaacaag cgcggcatct atggcggcgc
     1441 aataggatat atagatttta caggcaatat ggacacctgc atagcgatca ggatcgccta
     1501 taagaaaaac ggcaaggtgt ttgtacgaag cggcgcagga atagttgcag actccgttcc
     1561 cgaaaaggaa tatcaggagt gtataaacaa ggcacaggcg gtagtaaacg cactaaaaaa
     1621 ggctgaggag gcagatgtat gatacttctt atagataatt acgacagctt ttcatataac
     1681 ctttatcagc ttgtcggtga gatcgacccc gatattaggg ttatcagaaa cgatgaaatg
     1741 acggtcgaag agataagaca gctaaacccc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttctgtcacc
     1861 gggtccgggc agacccgaaa acgccggtgt gataatcgat gttataaaac agctcggcaa
     1921 ggatttcaag atactcggcg tgtgtctggg tcatcaggct atctgctcgg catacggcgc
     1981 aacgatcacc tatgcaaagg agcttatgca cggaaagcag tcggacgtaa cgtttgacaa
     2041 aagctcactg cttttcaaaa actgccccga taaagcaccc gttgcacgtt atcattcgct
     2101 tgccgccgat tctgcgacaa tgccggactg cttaaaggtc accgcagtga ccgatgacgg
     2161 tgagataatg gcagtacagc ataagaaata tgatgtttac ggagtgcagt tccaccccga
     2221 atcgataatg acacctgacg gaaagcagat gttgagaaat ttcataaagg agaattaaga
     2281 caatgatcaa agaagcgata gtaaagatag taaacaagga agaccttact tacgatgagg
     2341 cttataccgt aatgaacgag atcatgagcg gtgaaacgac cccgacccag aatgccgctt
     2401 tcttagccgc tctttcaaca aagagcgcaa aggctgaaac cacagatgaa attgcaggct
     2461 gtgccgccgc tatgcgtgac aacgcaacaa aggnnnnnnn nnngatacag gcatggatat
     2521 attcgagata gtcggcacag gcggtgacaa tgcgcaaagc ttcaacattt ccacgacctc
     2581 cgctcttgtt gcggcggcag gcggaatgaa ggtcgcaaag cacggcaaca gagccgcatc
     2641 ttcactgtgc ggaactgccg attgccttga agcgctcggt gtaaacattc agcaaagccc
     2701 aaagcgctgt atcgagctgt taaagaaggt cggaatgtgc ttcttcttcg cccagaagta
     2761 tcacacctca atgaaatatg taggtcctat cagaaaggag ctcggcttcc gcacggtatt
     2821 caacatctta ggacctctca caaaccccgg tagccccaaa atgcagttgc tcggcgtata
     2881 tgacgaatat cttgtagagc ctctggcgca ggtacttatc gatctcggtg taaagcgcgg
     2941 tatggtcgta tacggcaagg ataaactcga tgaaatatca atgagcgcac ctacagcagt
     3001 atgcgaattt aaggacggtt ggttcaggtc gtacaccatc gctcccgagg atttcggatt
     3061 tga
//
LOCUS       DAWAOG010000226         3056 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4139_length_3056_cov_1.914695, whole genome shotgun sequence.
ACCESSION   DAWAOG010000226 DAWAOG010000000
VERSION     DAWAOG010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4139_length_3056_cov_1.914695"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          783 a          708 c          642 g          923 t
ORIGIN      
        1 ccgtctatac ctctgatcgt cattattccg ccgccgtttt cggtttcact ataaaaggtt
       61 attgttgcat ctgtgccggc aataccgttc catacaaata tctctgcacc gttcttgagt
      121 atgacattta catttccttt gagatccttg tataatcagt taccgttccg cttacaacag
      181 taccgtctgc agacagtccg taataactca ccgattcatc ggcaattgcg tttattgcca
      241 taaaggacag cgagccgact gcaagcacgg cagatgttgc tatgccggct atgattctgg
      301 ttgaaaatgt tcttttcatt tgtctctcct tatccgggaa atttcaagta atgatatacg
      361 ttacttatct atcatataat tttaacattc cgtaaagaga atttcaacaa atatggcacg
      421 aacggtcaaa aaatggcggc gaatggtata attaccttcg ccgcctgact ataatgaatg
      481 tttacttatc aaaaccgagc atttttttca ctcttgcctt ttccagcgca acggtcagga
      541 gcacaccgac tataagtcct acagcaccct ggatcacgtt gccgaaaatg cttgatgccg
      601 ccgcaattgg atggccataa agaatgatcg catagaggta gtagcctgca accataatta
      661 tttcagctac tacgccgcct attatcttac cgacaagcga cttcatataa gaactgttca
      721 taaagctctt tgcaatgtag aatgccgcta ctgccataag cgcctttatt atcagcgttg
      781 ccggtgcata ataagcataa cctgtaagca gatccgccat cgcagagcct atgcctgccg
      841 ccgcaatgcc ccacggcgag cctaaaagcc atgcagatgt aagtacaagt acgtcaccaa
      901 agtttacata gccctttgta agcggcatag gtatctgcac caccattgtc gcaacacagg
      961 tcagtgcggc aagcacagcc gcagttacga taagcgtcag attttttgtc tttaccattt
     1021 ttctttctct tctttctttt gttttaggct tatttgcctt ttttcatatc attagcggaa
     1081 tatactacct ccgcatagat agccgcgatt attctgtaca gttcaacagg gatcgcttcg
     1141 ccgctgttga gcattacaag gagcgccgcc gtgctgtcgt cacggtaaac gggaacgccg
     1201 ttctcatctg cgatatttat aatcttcttt gcaagctcgc caagacccga cgctaccacg
     1261 accggggcat agttaaggtc ggggttgtat ttgagcgccg ccgcggcttg ctttcctaaa
     1321 ttacgcttgg acattaagac ccttcctcct gtcgattatt ttcgggaaaa cctcagtcag
     1381 cacatgaggc tttctcagca ccgcagtttc aaattttgcg ggcttatagc cgatattcct
     1441 tattataagg tcgatcttat cggcaagttt ttttatcttg aacccgaact tttcgggata
     1501 taaaagcgac agattgatgt tttcaccgag tgcgtacata tcgacctcaa agcgtcctat
     1561 tgactcaaca tcgaacgtca ggaacagatg atagttcttc tgccctatcg gcgtgttgtt
     1621 gggattgttt tcatcattat ctacccacag ctcgccaaat gctttggtgt cttctatctg
     1681 caacggcaga atgtagtgag caagaggagt gaatacaccg ggagcgttaa gcaaactttg
     1741 taaaaggttt gaagcattga aatcgtcaag cgactgtatc gcctcgtgat ttatgttctt
     1801 tcctatgaaa tcggtaagct tgtcgagcgt tgacggcgca gacggaatat tgctcgccgc
     1861 aggaagctct ttacgctgcg ccattttgtt tactatatcg gtgaaaaact caaagatcgt
     1921 atcgcgctga gggatatcgg gcatttcgcg aagtatctca ttaagataat caacgagagc
     1981 cgtcatatcc ttaagtccct gaaaatcatg atgaactgtc ggtatcaccc gacggctgtc
     2041 cgttcctgtg agcaatgcca ttactatatt tttaagtgtc tgtaatcccg attgtttgcc
     2101 ctcttcaaac ggagttgtca gaaattctag tttatccctt gataaatcta gttctgacaa
     2161 gtatttttca tcggttaaat gcgacttaat gaacaaagac aaggacaatt tttcatcgtt
     2221 attgtcaaaa atgcttaaat agtcttgatt ttcggactga tttagtgtat tattataaat
     2281 atcaggtgtg tccgacatct cactcagctg ttttttgctg aacatttttt tcagcagatt
     2341 tccaaatgct acagataatt cacgtcgcat ttcgtcagac ggtatatacg ccagaagatt
     2401 agcaaagctt tctttcagca taccgttatt tgtattgtaa cgtgagaggt tatgtacgat
     2461 aagcgggata aaagtttgtg ttctctcatc gttgagaaga cttgccgaaa catctttcaa
     2521 aagcgcaaga gtttcactct tcagcggctc aaacatctca ggcgcactgt tgcttttcca
     2581 ctgtaaggac agatccgaca gcttacgcga cagtgactcg ttcggcgaaa agtattccga
     2641 aagaaacttg aggttagcgc cgagagcgtt gagtatctcc tgtttgttag atgaatagtt
     2701 cagtgctttc aacagttgac cgatgctttc tttaagctcg ggaccggtgg tggtcttcaa
     2761 gatgccgtgc aacagcttat aaaactcatc gccactgaac atggtgttct gtttttcctg
     2821 gttctgtatc tcacgaacaa gcttctcggg tggaagataa aagctcttga taagctcatc
     2881 catatcgtcg tacagctcgg tgtaaccgtt tatcatcgtc tgctcgagca gatccgagct
     2941 taataggttc ttgagcgttt cgaccgccat tgtgggatct tttatctgaa ccgtgagcgg
     3001 agcaaggttt ttcctgccca tcatcatagc ggcagcagaa tcattatcct gtgcag
//
LOCUS       DAWAOG010000227         3048 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4151_length_3048_cov_2.119612, whole genome shotgun sequence.
ACCESSION   DAWAOG010000227 DAWAOG010000000
VERSION     DAWAOG010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4151_length_3048_cov_2.119612"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          771 a          748 c          635 g          894 t
ORIGIN      
        1 ggagcataca gcgttgctat tgcacttatg atagcgctga ttatcgtgaa aacgagatag
       61 aacgtatagg gctttgtata tttcagcaac tttacgagaa cggtaaaacc gctgtcatta
      121 ctttttttac tcatcgccgt ttatctcctt ttcgagttct tctgcactga actgtgtaag
      181 gcagatctga cagtaatccg tacaggtctg taacagttcc ttgtgcgtac ctgtaccgac
      241 cattttaccg tcatcgagta cgattatccg atcggcattc tttaccgtat ttgctctctg
      301 agaaatcagg aatacggtca tattcgtgca gttctccttg acagcagttc tcagctttgc
      361 atctgttgca aagtcgaggg cgcttgcgct gtcatcaagt atcagtatct ccgggtcggc
      421 ggctatcgct cttgcgatag taagtctttg cttctgaccg ccggaaaggt ttttaccgcc
      481 ctggagtatc tcatgatcaa gccctttatc aagcctttcg acaaactcat aagcctgtga
      541 gatcttcagt gcgtgccata tctgctcatc ggttatatta tctccgcccc acttcatatt
      601 ctcacggata gtaccgctga aaagcactgc tttttgagga acgacagcta tctttttacg
      661 cagatcgcca aggttgtaat tacggacatc tgttccgcaa acactgacag ccccctttga
      721 tgtatcataa aatctcggaa tgaggttgac aagcgttgat ttaccgctgc cggtaccgcc
      781 gataataccg atggtctgac cttttccggc agtaaatgat atatcttcaa gcgcatattc
      841 gctgttatcg tggtagctga atgacacctt gtcaaatctt acagccggag cagacgggtc
      901 gacatttcct tttgtcgttt cttttccttc tattgacggc tttgtatcaa gcacctcatt
      961 tactcttgcc gcgcaagccg cagattttgt gaagataacg acaaggttcg ccacgaccac
     1021 aagcgcaagc agtatctgat tcatatagtt tacaagcgcg atcacctgac cttgtgtgag
     1081 atcgcctaca tttaccgaga gcccgccaaa ccatataatt gcaacgatcg caaggttcag
     1141 tataatggca ttgacagggc ttaacagtgc tgagatctta cccgctctga cagccgcctt
     1201 ttcaatatcc tcagcgttgt ctttgaagcg ttcctgctca tattcctgct ttgagaaggc
     1261 tcttacagcc cttgcaccaa caagattttc acgcgttata agcgaggtct tgtccagctt
     1321 tttctgtatc gacttgaaga aggggatagt ccttgttgtg acaagccaca taacaatcgc
     1381 aacaagcgga ataacgagaa cgaaaatcag cgccaatttc gcatctatca taaaagccat
     1441 gatagtagag ccgataacca ggaaaggcgc tcttacaacc agtcttatca gcatcgcaac
     1501 agcgacctgc agctgattta tatcagaggt aagccttgta ataagcgatg gtgtaccgaa
     1561 ctcatctacc tccttgtgcg aaagggtatt gatatgatga tacagctcac gtcttagctc
     1621 cgtgccgaag ccctgcgatg cgaccgatgc cacatactgg catatcagcg cgaaccccag
     1681 tccgcataca ccgagcagaa caagcactcc acccatcttc catatataac cgctgtcctt
     1741 atttgcaatg cccacatcga ttatctgagc cataacaaga ggcactatca gctcaaatac
     1801 cgcctcggtc agcttgaata taggtccgag tattacgttt ttcttgtatt tttttagata
     1861 cttaaccagt ttccgcatat ttcaccgtcc aataataatg acaccggagc ttactccgat
     1921 gtcatcaaaa ttattcaata attatcatct gagaattttt ctgatttcct caggaagagt
     1981 ggtagcaaag ctgtcgccta ccagcaggct gatctcgtca acgctcgttg ttatgagcat
     2041 tacatcggcg caaatgtttt cacaagcctc aaatacctta gccgcaaaac ctgcctggtc
     2101 taccatttcc tcgctcttga taacgatctt tacgttgccg ctgtttacgc atggcgtaat
     2161 tccgtgtaca gccgtgattt taggtaaaac agtgagcaat gtaggaatat catcatcgct
     2221 gaacgtaaag ccgaatgaga acatatcgga ttttggcggt gtctgagaga tcatatcgat
     2281 gttgatcccg ctttctgcta ccgctgtaag cgtagtcgaa acaaaacgct ttcttgaggg
     2341 tacgtctgaa aatgtcacga tagacacgtt ttcgtatact ttaatatctg tcatattgac
     2401 ctccgttata cttcggaaag aagatccgac atctgataaa gacctttttc acagtcgctt
     2461 aagaatactg ccgcattgac agagccgact gcgaatacct gctttgagcc tgcactgtga
     2521 gaaagagtga tgacctcgtc acgtcccgca aatataacat catgttcgcc tactatagta
     2581 ccgcctctta acgagtgcat accgatctcg gtcttttcac ggggctttct cacgctgtga
     2641 cggtcatata catagtgcat cttgttgccg agctcttcat ttatcgcttc cgctatcatt
     2701 atcgctgttc ccgaaggcgc gtcttttttg aggttgtgat gcttttcgac tatctcaata
     2761 tcaaactgat cgccgagtac ctttgttgca cgctttgcaa ggtcaacaag aagatttatt
     2821 ccgagtgaca tattgaatgt gaagaatacc gggatctgat ctgccgctgc ctttatctgc
     2881 tttatctgtt cttctgtgta gcctgttgtc gctacaacta caggaacatt gtttatcttg
     2941 cagtagtcga gcagttcatc aagcagtgag gggtgcgaga agtcgattat tacatccggc
     3001 ttttccggta aatcgtacat tgttttatat acagggaagt ctgcatac
//
LOCUS       DAWAOG010000228         3041 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4171_length_3041_cov_2.128935, whole genome shotgun sequence.
ACCESSION   DAWAOG010000228 DAWAOG010000000
VERSION     DAWAOG010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4171_length_3041_cov_2.128935"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1874..1904
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          897 a          692 c          755 g          666 t
ORIGIN      
        1 acttaggaac ggaaatggcg cacgagcttt atttcaagat agaaaaatat cttatcgaaa
       61 gcggcgttga ggtgctgttc ggcaagaact gcgaggatat tataatagaa gacggcgtat
      121 gtaagggagt tatcatttca aatgcccgtg acggcggcga acaggaaacc atatacggtg
      181 acgagatagt ggtcgcaaca ggccgtaagg gcgcagactg gcttgaaaag acctgcgagg
      241 ctcataatgt agagcataca ccgggtactg ttgacatcgg cgtaagagtg gaagtcagaa
      301 acgaggtcat ggaggagata aatgccgtcc tttacgagtc aaagctcatc ggctatcctc
      361 tgccgtttaa gaacaaggtg cgtacattct gccagaaccc cggcggattt gtaagccagg
      421 agaactacga caacgattta gccgttgtaa acggtcactc atataaagag cttaagagca
      481 acaacacaaa ccttgctata ctttgctcgc acaatttcag cgtaccgttc aaccagccta
      541 tagagtatgc gaagaaggtc ggcgagctta ccaatatgct cggtaacggt cacattcttg
      601 tacagcgtta cggcgatatt cttgacggaa agcgtacatg gcccaaggag cttaatttct
      661 ccaacgtaag acccaccctg cccgatgcgg tcgcaggcga catcacggcg gctatgcctt
      721 accgtacaat gacgaacatt atcaatttca taaaggctgt ggatatggtc gttcccggtt
      781 ttgcaagccg tgaaacgctc ctttattcac ccgagctcaa attctacagc aaccgtataa
      841 agatggacga gcatttcaac acgaacgtaa agggactgca ttgcttaggc gactcaagcg
      901 gctggacaag aggacttatg atggcgtctg taatgggcgt acttatggga cgtgaattac
      961 tgaaataaac ggaggaaaag taaaatgcag ttactggaag atcgcataaa caaggacggt
     1021 atagtaaaag aggggaacgt actcaaggtc gacagctttc tcaatcatca gatggatatt
     1081 gagctttaca acgagatcgg caaggagttc cgcaggctgt tccccgacaa gagcataaac
     1141 aagatactga ctatagaagc gtccggcatc ggcattgcgt gtatcaccgc gcagtatttc
     1201 ggatgtcccg ttgtatttgc gaagaaggcg cagagcgtaa acatagacgg cgaggtttat
     1261 tcgacctcta tcgagagctt tacgcacaag cgtacatata acgtaatagt cagcaagaag
     1321 ttcctgtcgc ccgatgacaa cgtgcttata atcgacgact ttcttgcaaa cggctgtgct
     1381 ttactcggtc ttatcgacat agtaaagggt gcaggcgcaa gtatagcagg cgcaggtatc
     1441 gttatagaaa agggcttcca gaagggcgga gaccttgtcc gctcgaaagg catcagagta
     1501 gaatcgctcg caatagttga ctcgatgagc gttgacggcg gctgtatttt cagacactga
     1561 gccgtaaata accgtattta gcggcaaaat gccgttaata tatcttttac tgttttaata
     1621 gggaggaaaa acatgaaaaa gaaacttatc gccattttac tggcggcaac ctgcgcactt
     1681 acagcaggac tcacaggctg ctccggcaat accgcaacaa gcggtacgga tgaaagcaag
     1741 acttccgacg cacagagcac aggcgacagc tctaaggaga gcacaggcga cagcacagcc
     1801 aagagcgatg ttgtagcagg ctttgtttac atcggcaaga tcaacgacgg cggttacaca
     1861 caggctcacg atgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaggcag acttgcagtt
     1921 gaggctatgg gcgtaaagac ctactacgtt gaggacgtac ccgaaaccgt tgccgacaca
     1981 aaggaagcta tccagacact tatcgatgaa ggctgcaacc ttatctacac aaactcattc
     2041 ggctttatgg aaggtacaga ccagatggca aaggagcatc ccgatgtaaa gtttgctcac
     2101 tgctcaggct atatgagaga agacaatatg tcaacctact tcggtaaggt atatcaggct
     2161 cgttaccttg caggtatcgt tgcaggtatg aagacacaga agaactacat cggttatgtt
     2221 gccgctaagg gtatccccga ggttatccgc ggtattaacg cattcacact cggtgtacag
     2281 tctgtaaacc ccgatgctaa ggtagaagta gtattcacag atacatggta tgaccccact
     2341 gttgaaaagc aggcagcact tgagcttctc aacaagggtg ttgacataat cgctcagcac
     2401 caggatacaa cagctcctca ggttgccgct gaagaaaagg gcgcatactg catcggctac
     2461 aacttcccca catcagacgc tgctcccaag gcttacctca cagccgcttg cttcgactgg
     2521 aagacattct acacagacga tgtaaagaag gctatggacg gtacatggac aagccgtgca
     2581 tactgggaag gtcttgccgc taacatgaca taccttgaca agcttacaga cctctgcgca
     2641 gaaggcacac aggaaaaggt cgatgcagct cagaaggcta tcatcgacgg cacacttgag
     2701 cccttcacag gtcctcttta cgaccaggac ggcaacaaga aggttgaaga cggcgttaag
     2761 atgacagacg acgagatctg gaacatgaac tggttcgtta agggtgtaac aggcactatc
     2821 cccgcataat cgaaaaacat caagacaata cgagcagtcc tcagtgactg cccgatttgt
     2881 ctttttaaag caccggaata tctgcgacgc tttaaaaaga caaatcgctt tatttcagga
     2941 ggttatatgt cagttcctta tataaaactc gagaacataa ccaaatcgtt tgactcggtg
     3001 tacgcaaaca aggatatttc cgttgacgtg tacaagcgcg a
//
LOCUS       DAWAOG010000229         3020 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4207_length_3020_cov_2.053288, whole genome shotgun sequence.
ACCESSION   DAWAOG010000229 DAWAOG010000000
VERSION     DAWAOG010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4207_length_3020_cov_2.053288"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          753 a          777 c          542 g          948 t
ORIGIN      
        1 tgctcttcta tcattgaaag taatgctctg catttgctct catcgtgcag gaaaagctcc
       61 agctgatagc ccttttcggg gtttgccact attccgcata caagaaatat ccccctgaga
      121 tacacgccga acgcgtcatc tccggagcag tcgggcacag gataagcata ccccttatcg
      181 gcaagcgtca ccgtatacag agtctttcct ctgaaagtgc tctcgcctgt gtcatatctg
      241 acaccgacag cctcgaacgc atctgcgata caatccgcgg ttttcttgtt ttccgtatta
      301 aaaaaacagc cgtcgccgtc ttccgtcatg gcgaatgccg cgcccttgga caaaagctca
      361 agctgttttc tgctgtgttc cgccttgcac agctcgtttt ttacatcggc ggcaaatgac
      421 attcggatcg ctcctttatt ttactttgca aaattacaat aatcctcggt tataaccaga
      481 tcaggttttg cggtaagttc gttatcaact ctgcacaaag gataaaactc agcataaagc
      541 cgctttgtac cctgatatct gttaccggat acattcttta tgccgtatat ctcctttata
      601 ctgtacggca gatcgaagag tgacgctctt gtcgctatta cataataatt atcggtattc
      661 tttacagctc ttgcaaagtc ctccgatgcc acaaacttat cgccctcgtc tatgaacacg
      721 atactgtcgt gtatcatcgc aaggttttcc tgccaacgta tgcctgtaag caccgtacac
      781 tgttttttac tctgcaccgt aacgccgctt gcttcgccgt tttcctgata tgccgctatc
      841 atatctataa gtgtagtctt tcccgttgcg ctgtcaccgc gcaatatagt gatatttctc
      901 aatactgtga acttatactg aatacgtcgg ctttttacgg taatattata cttccctttc
      961 atgcttcacc gccttacaga tacattcccg cattcagcac atattccttc atattatgaa
     1021 cagtcttacc gttgttgagt atctttatct caaacggctt atcaccgaaa ttcataatat
     1081 gcctgagatt tataattata tccctgtcgg ctttctgtgc tattttcagt atccattttg
     1141 cgcaattgtc gccgcaggtt gaagcattga aaattttggt attatcgtga cggataagca
     1201 gcagtgtttt aagtccgccc gagatctttg ttaccggaat aacgcccagc gctttgctgt
     1261 ctacagcatt tgaactcagc accacagcct tgtccacgct ctttatcatt ttaaccgaaa
     1321 gctcatcggt aagccagttg tcaagatagg tattgtcaaa atacatactg gtgttataga
     1381 cgtatttctt ccccatatct tcgggcattt ccccgaaaat aatacacagc ataccgttca
     1441 cctccgctga aattttctct tccctttact tcttatcctt atttacatct ctgtgaagca
     1501 cccttgcttt aagatgattt ttactgagat gctcgcacat attctccgca aatgttgcac
     1561 tgcggtgctt gccgccggta cagccgaaag ctataacgag ctggctcttg ccctcatgca
     1621 gatactgcgg aataagaaaa tcaataagat caaacagctt tatctgtaaa gtctgcgaac
     1681 tttcaaagct cattacataa tccctgacct ctttatcaag cccggttttt tccttaagct
     1741 cgggtatgta gtagggatta ggcagacagc gcacatcaaa aacaagatct gcctcattcg
     1801 gaacaccgta cttgaaaccg aacgagatac agcttatcgt catactgtct gacggcgtat
     1861 caagaaagat attgagtacg ttttctttca gcgttgaggt cgacataaga ctcgtgtcaa
     1921 tatagtaatc ggcttgctcg tgcaacggct cgagtatctc tctttcaaca tcgatagcct
     1981 tatcgatatc accgtcagca acatcaaaca gcgggtgctt acgccgtgtt tccttatatc
     2041 gctgtcttac cacatgatgg tttgcgtcca catataaaag ctttacatca agtccgcgct
     2101 ttttaagctc ttcaatagta tcgcttagat taagaaacat agttccgccg cggatatcgg
     2161 taactatagc cacctttgat atatcctcgt tttcctcaca tatctcggca aatttcggta
     2221 tcagctgggg tggcatatta tctatacaga aatagccgat atcctccatt acatttaccg
     2281 cacaggattt gcctgcacct gaaatacccg ttactataac aaattcggtt ttcatttgtg
     2341 ttcctttccc tgcgcccttt tatctgttta atgaataatg aataatgaaa aatgaataat
     2401 tttggtgtgc cgctttgttt gcttacccgg ataactctgg gtaaaccata agcttatctc
     2461 acccggtaca cacatctcag gcacttatta aaaattttca cttcattata agcacttcgc
     2521 attcaagcat tacacctgtc tgctctttca ccttttgttt taccgtatcg acaacgccgg
     2581 atacgtccgt catcgaataa tgaaaaatga ataattttgg tgtgccgctt tgtttgctta
     2641 cccggataac tctgggtaaa ccataagctt atctcacccg gtacacacat ctcaggcact
     2701 tattaaaaat tttcacttca ttataagcac ttcgcattca agcattacac ctgtctgctc
     2761 ttttaccttt tgttttaccg tatcgacaac gcccataaca tcgtcaaatg tcgcattgtc
     2821 cttgtttata acaaacccgc tgtgctttgt gctgacctca gcccctccgc aggcagttcc
     2881 tttaagtccg cagacttcga taagccttgc ggcaaagtcg ccctcagggc gcttgaaggt
     2941 actccccgca cttggatatt caagcggctg tttatccttc ctgcgctcca tcagcgtatc
     3001 catacggtct tttatcttac
//
LOCUS       DAWAOG010000230         3006 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4234_length_3006_cov_2.385293, whole genome shotgun sequence.
ACCESSION   DAWAOG010000230 DAWAOG010000000
VERSION     DAWAOG010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4234_length_3006_cov_2.385293"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    951..960
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          648 a          788 c          645 g          915 t
ORIGIN      
        1 tacggttatt ccgcatatcc ccgacagtgc cgtgccgcgg accatattta taagtatggt
       61 ttcgcagtta tcggacgctg tgtgcgcggt agctataagc ttgccgcggc ttatgctgtc
      121 gaaaaattcg tatctgagcc gtcttgcgca ttcttcaagg ctgtcgtgct tttgtctgag
      181 atcgtttacc tttatacttg ctacatacag cggaatatca agaaatctgc acattctgcg
      241 gacaaatctc tcatctccgt cgctctcctc gccgcgcagg ttatggttta catgacaggc
      301 ggcaagcgaa aatccgagct cgtttttaag cgaatacagc acatacagca aggcgacgct
      361 gtctgcgccg ccgcttaccg ccgcaattac agtatcgccc ttatttatca tcgaataccg
      421 ctctatcgta tttttaacgg aattttttaa atcactgctc ggcatggaca tactccacat
      481 ctgtaaatct cgtgtactga ccgtcccata tcatattgat atcgttggtt tcgccgtgac
      541 ggttctttgc gactatacac ttgcaggctc tctgatcctc agcttctttg ttatagtaac
      601 tgtcacggta caggaaaagg actatatccg catcctgctc gatagaacct gaatcacgca
      661 ggtctgacac cataggttct cgacacggcg gagctttgcc ttcatctcgt ttaccgtgat
      721 cgccgagctg tcgtctatat agatattcat atttgacagc acgaatgccg catcggcaag
      781 gtttctccac tgtgcggggt caaagcgacc cgttgacata agctcgctcg aaattctgcc
      841 ctcggaggac agcatacgct ttacaagctg ttccttagac atttcaaggt taaatacaca
      901 cactgccttt tcggggtgat acttggctac gtttgtcgcc atattcatgg nnnnnnnnnn
      961 attcatggca aatgacgttt tacccatacc gggacgcgcc gctacgatca gcagatccga
     1021 attattaagt ccgtgtatgt acttgtcaag tgacgggaaa cctgatttca gtccctgtat
     1081 gccctgtctg tccggatttg cacaaagctc ggacagctca ttaagtatgc cgacaacgat
     1141 gccgttcagc tttgtaagac ctctgacctg attttcgttg cgaacctcat atattttctt
     1201 ttccgccagc tcaagcagag tagatgtttc tgcattaccg tcgtttgcgt tttcaaggat
     1261 atcaccggct atgcttataa gaagccgcct tgtgtactta tcctcgacta tctcgatata
     1321 tctgtctatg cttgaaatgc tcgggacggt ttccataagc tttacaagat atgtacgtcc
     1381 ctcttcctgc gactcgaaaa cgttctgctt catacagttt tcaagcactg taacaacatc
     1441 tatgctgtcg cttgcggcaa acatctgcgt cataacggtg taaatgtcgc tgtgcatttt
     1501 gacatagaaa tgctcggggt gcagctttgc cgatgccttt ggcatattgg cgctgccgtc
     1561 aagcagtata gcaccgagta cagactgctc cgcatcaagg ctgtacggga gatttacccc
     1621 cgtaagctcc atatcagcca ttattcttcc tcaaccttta cgctgaactg tgcaacgata
     1681 cctgtaaaca gtcttgcttc tgcagtgaat gtgccgaatg acttgatatc gctctttgtt
     1741 actatctttc tcttgtcaac gtcaaagccg aaatcacgct ttatcagtgc gcttatatcc
     1801 ttgccggtaa cagaaccgaa cagctttccg ccctgtcctg cttttgcctt gattgtaagc
     1861 accttgccgt cgatagtatc ctttatcttc tttgcgttag ccgcttcaac atcaagcttg
     1921 tgctgtgcag acgccttctg accctcgagg tggttaaggt tctcgttggt agcttctacc
     1981 gcaagtccct tcttgataag gcagtttctt gcgtatgcgt ccttgacttc cttgacctcg
     2041 cccttcttgc ccgagcctgc tacgtcttgt gtgaaaatta ccttcatttt atatcgtcct
     2101 ttcttttaac tgcgctgtgc agtcttgttg tcgtttgaat ttacctttat cagacttaaa
     2161 tagtaatcgt ctattgattt tttgagtacc tcgagcgctt cgcccagtgt ttcgtcctta
     2221 agctgtgccc ctgccatagt ctgatgaccg ccgccgccca gagcctccat aatgagctga
     2281 acatttaaat tgcccatact tcttgcgctt atcgatatct cgttgctcat cgtcttgtac
     2341 agtacgaatg atgcgtcgac attcttgata gtgagcaatt cgtccgccgc ctgcggagca
     2401 acgatcctca agtcgtccgc atagaagtcg cagggtgcta tcgcgcactt gcgatagatc
     2461 tccgcttccg ctactatctg ggtctttcgc ttatagctgt ctatggagct tgagaacatt
     2521 tttttgacgg ttatcgtatc tgcacccatc ttgcgcagta cagccgccgc ctcaaaggtt
     2581 ctgacaccag ttttcataac gaaattcttg gtgtcaagca taattccggc aagaaggcac
     2641 tctgcctgta ccgctctgag cttaccggct tcgccgaagt actgtataag ctcggtcacc
     2701 atttccgatg cggaggacgc atacggctcg tggtggaata ttacggcatt gtctatgtag
     2761 tttaccatct tcctgtggtg gtcgatgaca actaccttct ttgccttttc gtaaagctcc
     2821 ttgctttcga ttatcagcgg gttatgagtg tcgcatatta taagcaaggt gttatccgtt
     2881 gtttctccga ccgcatcgga aggctccata aacaacggtt cttctccgtc aacgtgcgga
     2941 aatctgtcta tcagcacctt tgcaagtgaa gtgttgtagt cacataccgc ccatgcactc
     3001 ttgccg
//
LOCUS       DAWAOG010000231         2996 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4256_length_2996_cov_1.908194, whole genome shotgun sequence.
ACCESSION   DAWAOG010000231 DAWAOG010000000
VERSION     DAWAOG010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4256_length_2996_cov_1.908194"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2726..2735
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          832 a          599 c          727 g          828 t
ORIGIN      
        1 cacttaaata tgcggaaatt gggagaaata agagatttca gagcggaaaa gaaaaaatct
       61 gaaatgagaa tttattttga gaaatttgag aaaagtgctg aaatgagaat ttttcttgac
      121 attctgaaca ttttgtgcta atatgagcat ataatataat agtacagaac gataagaggg
      181 tgattttttt ggttgaaaga aaagaatatc ttgaaaagct gaagctctgg aagggcgaac
      241 aggttataaa ggtagtgacg ggaatacgca ggtgcggaaa atcaacattg cttatgcagt
      301 atcaggacta tcttaaacag agcggagtag aagctgacag aataattgcg ataaactttg
      361 aagaactgga atacgaagaa ctgtgcgact ataaaaagct gtatgaatat ataaaggctc
      421 ggctaagcag tgataaaacg acttacattt ttcttgatga gatacagaaa gtgccgtatt
      481 ttgaaaaggt ggtagacagt ctttatgtaa aacagggaac tgatatttat atcaccggct
      541 caaacgcata tatgctgtcg ggtgaccttg ctacacttct cagcggtcgt tatgtggaaa
      601 tatcaatgct tccgctgtcg tttgcagagt atttgcaggt aaacagcaag gacaaggaca
      661 gtgcatttgc tgattatatg agatacggcg gtatgccttt tattgctatg gcagaaaaga
      721 cggaagagaa gataactacc tatcttgagg gtatctacaa tacggttatc gtaaaggata
      781 ttgaggacag gcagaagcgt aaggaatggg ataacagcga taaaggcagg ataacggata
      841 tatcgcttct taaaactatc gcaaagtacc tttcaagcgt tatcggaagt cttgtgacgg
      901 taaggagcat tacaaattat cttgtttcat cgggacggaa aatttctccg aatacggtga
      961 gcgattatgt taatgcactg accgaatcat tcatattcta cccggcggaa cgctttgaca
     1021 ttgtgggaaa gcaacttctt aaaaccaaaa cgaagtatta tatggttgac ctcggcatac
     1081 gaaatcttat actgcctaag aaaagctatg accttggctt ttcggttgag aatgtagtgt
     1141 attttgaact gctcagacgt gggtacaagg tgagcgtggg caagtacgga aataccgaga
     1201 tagattttgt ggcagagaga aacggagaac ttgagtatat tcaggtaact gccgatatga
     1261 cctctaagga aaccttcgat agggaacttg caccgcttac aactataaag gataactacc
     1321 gcaagagagt tctgacactt gataaattca ctgtcggaaa ttatgacggc attgaggtga
     1381 agaatgttgt cgagtggttg acggataaat gatgacagaa tatgataaag ccgcccgttt
     1441 gcatagtcag acgggcggcg ttttatattc agttgttttc gatttcggtg ataagctgta
     1501 tcataccgct gataagcctt ttgtgttcat ctgtcatagg ggttatcttt gaggcgatgt
     1561 cattgtcata aagctctgcg gaagcagtgc cgagcaggaa atagttgggt gtcgtattca
     1621 gcacggagca aaggctcatt ataagctcaa gactcggtgc gctcctgccg gtttcgatat
     1681 ttgagagata tttatagttt acgtctatca gctcgcagac ctcgtactgc tttaagccga
     1741 gttcctttct gcgctttgct atgcgttttc cgagagcggt atagtcaatt ttcatagtat
     1801 cacctcaagg atttattact gatattctaa tacaaatccg ctcgcttgtc aacacaaaaa
     1861 ctccccgcca acccggcggg gagttcgcat ttcttatatt acctgtaacc cttaacctaa
     1921 aacaacctca accttgtgag tgccgttttc aaatacgggg ataacgttgc tgtcaacagc
     1981 cttgccgtca actgtaacgg acttaacgcc gtggcaaacg tgatcggggt tctttacagt
     2041 aatctcgtag gttgcacctc tgaacagtct tgaagctgtg aagccgtccc actcgtgagg
     2101 aattgaggga tcgatcttca gaccgttgta ttcgggcttg atacctaaga tgtactgaga
     2161 taccgcaacg aagttccaag cggctgtacc tgtgagccag ctgttcttag cttcgccgtg
     2221 acgcttagcg tccttacctg cgatcatctg agcgtataca taaggctctg tgcggtgcag
     2281 atcggagata tcctctctga atgcgggagc aatctttgaa taatactcga atgccttgtc
     2341 acctctgcct actgcggctt cagcgcagat tatccaagcg ttgttgtggc agaagatacc
     2401 tgcgttttcc ttgtaaccgc cgggatatgt ggagatctcg ccgtactgga tgtagtagtg
     2461 tgtgaatgca gggttgttca gaacaagacc gtgctttgag ttaagatact tgtctacgct
     2521 gtcaagggtc tgaatatcgt agcccttgtc cttgccaaga cctgcgagaa cgcaccaacc
     2581 ctgaggttcg atgaatatct tgccctcttc gcactcgtgt gagccgacct tcttgccgta
     2641 gtggtcgtaa gcacggagga accattcgcc gtcatagccg tattcaaggg tgttctttct
     2701 catcttttcg atctcagcct gagccnnnnn nnnnnccgtg tcacccttca tcttgcacat
     2761 ttcaacgtac tcgggagcta ttgcacagaa catacctgcg atcattacag attctgcaac
     2821 cttgctgtag taaggaggct ccttgaacat ttccttgttg ttgtatgtct ggaatgactc
     2881 accgggcttg tctgagaagc aggagaggtt aaggcagtcg ttccagtcgg cacgtccgct
     2941 gagcggcagt ccgtgaggac ctaccttctt aacaacgtgg tagaacgctc tcttca
//
LOCUS       DAWAOG010000232         2992 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4262_length_2992_cov_2.474634, whole genome shotgun sequence.
ACCESSION   DAWAOG010000232 DAWAOG010000000
VERSION     DAWAOG010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4262_length_2992_cov_2.474634"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          705 a          807 c          583 g          897 t
ORIGIN      
        1 gtattgtact ttccgactac ctgtgcatta cctgtcagcc ccgcctttac cttaaggcgg
       61 aatgcaaact cgggcatctc cttttcatac tcgcggctta tctcaggtcg ctcgggacgc
      121 ggacctacta ccgacatatc gcccttgaag atattgaaca gctgcggaag ctcatcaagt
      181 cttacttttc ttatgaactt acctaccgga gtcactcgct tgtcaccgtc acttgcaagt
      241 cttgcaacgc cgtcactttc ggcatttacg accatacttc tgaatttaag gagcttgaac
      301 tccctgccgt cctttgtaag tcttacctgc ttatacagca caggtccgcg gtcgcatagc
      361 tttatcgcta ttgccgttgc cagcataaac ggagaggcta ttataatacc tatcgatgac
      421 agaaggatat caaagcagcg cttgaagaac ctctgttcta tacccagtcc gtactttttt
      481 gagataataa gcggtgtatc gaacaattcc atattatcgc cgcctcttac gataatatcg
      541 cttatcttag gtacaaggta agtccttatc gatgtcttga agcagtattt aagtatgtcg
      601 ttcctgatct cgttcggagt atcgcatatc accacgccgt cataattcgg tatctccgca
      661 gtaatagcct ccagcccctc ggaaacattc atcatacgag tgatattgta cttatcgcga
      721 cgactgctca ttttttccac cagcattctg gcaggctgtt caagtccgta tatcattatc
      781 acgttctttg cgggatacaa cgcacggaat atttttccgc tgataaacgc ccacagagct
      841 atcaccaccg cctgatacag cgtaccgaca ataagcgggc acggatcgag catagagcgc
      901 gatataaggc atatctggat atacgttata aaggttgtac agagcgttgc gaggatctgc
      961 gacactatga tatcggttgt cttatgagag ccgattttaa gtcccttata tattcttgaa
     1021 aatacataaa gaatagcaaa gtacagtccg aataacagcc agtttccttt tctgaagaag
     1081 ctgaaagcca tctcgctgtt atagtaattc agccatataa tgccgaacgt agaggtttcc
     1141 caggtaagta gcacgagcgc gtccactatc agcgcggtgc gtttgtattt ttctttatcc
     1201 ttgttcaata cgacacatcc ttaattactg cacacctgtc agtagtttat aatcgaattt
     1261 agtatagcac aatttgtcgc aataatcaag actttagctg tgatatcaca aaatcctcac
     1321 cttatactat cgaaaagcaa tgccgtcccg ctgttgggtt attacagcat gatatgtccc
     1381 ataagctgta cgccccgtat tgccggacgg caacacgggg cgtactttgt attgatataa
     1441 ttttaaacct tttcgcttct gaatcttccc acgaccttag cgttcttatc gatattgact
     1501 tcaaaacgca gagtgcaggg agagtaatgg ggattttctc cttcgcctat aacctcatag
     1561 aagaacgcaa gcgcaccccc gtcctccata cgtcttaccg cacacttgca aaatccctcc
     1621 gatatagcct gctttacata aggtatgtcg cggtcatatg tgcagaaatt atcctcggta
     1681 acagggatat tcacatcacc gtccgaacag ctgttttccc tgagcattgt ttcaagcgga
     1741 gatgtctttt tgcggctgaa ctctgcaaac ggagctttct tggctatttc gcccttgcgc
     1801 gcgggttttc cgtttatcct ccagcacgga cgcgtaacaa cgaccgtttc accgatatgg
     1861 aagtcattgt tcaggtgctc gctctgcgcc gaatagaaat ccagtgtatc cttatcgata
     1921 acaacttcct tcgccttgtc acggactatc ccgacaagct caagcataga gagcagttct
     1981 cccgcctttt ccatgcactt ttcgtccgtc catatctcgt agttgcggaa attatttctc
     2041 cagtatgtct ttatcggcaa atcaccgatc agtgactgta ttctcatttc ccagtcaaac
     2101 agtataccgc tcttactgcg tatcttgttt tcctttatgg cattgagcgg ctcaagcagc
     2161 tgtgcaaatg atgctatatt atcagcaata tcctgagcta tgtatttctt cgtatcggtt
     2221 gtttttttct tctcgttctc ctcttctatc ttctcatcat tgtatttatc gaaatcatcc
     2281 tgacttgcat cggtatcaaa catcgagaat acttcggcag gatcagagag cgtttttttc
     2341 tcatcgggct catcgtcgat aagcaaatct ccgaacagat cctcaacgtc agcgacattt
     2401 ccgccgctct cttcttctgt gggcgcgacc gtcttatacg cttcatcggg aagatcaaag
     2461 aaatccagcg ttctgggctt ttcctccgtg ttcttatcca actcgttacc gggagcttcc
     2521 tcaagcaggt catccgcctc accgtcagac tcctcggtgc tgttttcaaa aaccgccgta
     2581 ttcacaacag gctcaggcac acttacagtt ttttcgggta ccagatcggc gaacagatca
     2641 agagctttgg cgcttacttc ttcggatatg tcttcggaag tctgtccgat aatcccgctg
     2701 agctccttta acgcatcttc aacagactca cctgtgcgta tcgccgcagt ttcatctgca
     2761 tcttctgtct gctcctctat ctcctcaaac gcctttaccg cctcttcgac aggctcactt
     2821 gcaggaacgc tctcagccat cgcctgcgca aactcttctt caagcgtttc cgccgcttct
     2881 gccttttcct cttcgcttga tttttcctgc tcctctattt cctcaaatgc ctttactgcc
     2941 tcttcgacag gctcacttgc agaaacgctc tcagccattg actgcgcgaa ct
//
LOCUS       DAWAOG010000233         2981 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4281_length_2981_cov_2.348941, whole genome shotgun sequence.
ACCESSION   DAWAOG010000233 DAWAOG010000000
VERSION     DAWAOG010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4281_length_2981_cov_2.348941"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2195..2204
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2759..2768
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          967 a          635 c          708 g          651 t
ORIGIN      
        1 caagaaacgg acgtaaggtt cttgcaagaa gacgtgcaaa gggcagaaag cacttaagct
       61 actgattcag tcaatcaatg agtacgcaaa gagatacgtc tgcataaaaa aggacaggga
      121 gttcaaattt ctgttcagaa aaggcgacag tgtcgttacc gatgcattcg tatgctacgc
      181 aagggaaagc aagaggcggg taaaccgcat agggatcgtt acaggtaaga aggtcggcaa
      241 cgcagtaaag cgtagccgtg ccagaagagt catcagagaa gcgttccgcc ttattgaccc
      301 cctgctccgc gaaaaaacgc aaaaaagata cgactttatt ttcgtggcaa gagcaaagac
      361 tcctgctatg aaatcaacac aggtatacag gcagatggaa aacaaactgc ttaaaaggta
      421 tgccgcgcag gaaaataaag ctcctgcgga aattcaaaaa ataaaatgaa atatgtactt
      481 ttatttttcg taaagatata cagaatgact ttgtcaaaat ggctgcctcg ttgttgccgc
      541 tttactccga gctgttcgga atatgcgatg atcgcactcc gccgcttcgg tgcggtaaag
      601 ggcagctatt tggctatacg gagaattatc agatgcaacc ctttttccga gggcggctat
      661 gactatgtac ccgaacaatt ttattttttt aaaaggaaca agccgcaaca tcggcacgac
      721 taacggaggg tgatttccat agatttctta tacaccataa tcggtacgcc tctcggctac
      781 atcatgtggc ctatttatct tcttttacag aatttcggtt gggcgatact gatatttact
      841 attatagtca aaatagcaat gctgccgctt cagataaagc agcagaagaa tatggcgttt
      901 tctcagctgt ttgctcccaa ggtaaaggaa atacagcaga aataccgcaa caaccgtgaa
      961 aaacaggcag aagaaatgca gaagctgcag gcacagggct ataaccccgc aggcggctgc
     1021 ggacctctcg ttctgacaat ggtaatactg ttcggtgtca tcgacgttgt atataagccc
     1081 atgacacaca tggagcattt aagctcaaca gatatatcaa gcaccatcac agttgcagaa
     1141 gaaacagagt atgcgaaaat tttcgttaac gaatacaaca agcaggacgc tgagcttatc
     1201 ttaaagtaca agaacggcga aacggacgct atgttcatct ccgaatcaaa cgacagtgca
     1261 cagctgacgc atgagttcaa ggacgatgag aattacaaga acagctactc tgacaaagat
     1321 cccgaaaaaa tcagcaaaac ggatcgtgaa acatacggtt cattcactac cgaacagtat
     1381 gcagatataa ccggtgataa gtcaagactt acagctgaaa caaagaacag acttgcggct
     1441 gttgcaggca agtacaaggg tatgcaaatg gagcttcttg ctctgcaggt ttatgagcag
     1501 tatcccgata cattcaaaga aagctcgatg ctctcagatg acgtaaagga cagacttgca
     1561 gcactgtcac acaatatgat gttctgcggt ctgaacttcg gttctacacc taagctcgca
     1621 tttgaaccgc tgataatcat accgatattt gcatttatac tttcacttgt tcagactgta
     1681 ctttcacagt acatcagcaa aaagaacaat cccgagatgg ctaacgcggg cggtgcgggt
     1741 atgaaggtaa tgctttatat catgccttta ttctcgctgt ggatctcgtt cagcgttccc
     1801 gcaggcgtag gtttctactg gggtatcaac tatgcgctcg gtatagtaca gacaatagtt
     1861 ctccagaagc tctacagtcc cgataagctc agagccgagg ctgaacagaa gatgaaggaa
     1921 agaaagctcc aggaaagaca ggtcacaaca acagctgttg ttacagacgc cgatacaggt
     1981 gaagaaaaga cagtcacaaa gacagaaaca ctttctcaga aggaaataaa cagacgtaag
     2041 ctcgcggcgg caagaaaggc cgatgcagag aagtacggag aagaatacaa cgatgaagac
     2101 gacgattgag aatcgtgcgt gaaaggacag acttatgaaa aagatatata caggtaagtc
     2161 gcttgacgaa ctcaaggcgc ttgctatttc ggaannnnnn nnnnaaataa aagaactcgg
     2221 tgcaaacgaa gccgatataa agatcactgt tgtagaagaa cccgtaaaaa agcttttcgg
     2281 taaaaagggc gattacaaga tagaagccga atatgaaata gcagtaagag aagaagctcc
     2341 cgtagcagtt gcagcagtgc ctgctaccga agcagcagta gctcctgccg ccgctaaaga
     2401 cgcggatcac accgaaaagg tagctatggc tgcggcttat cttgaaaagg ttcttaccgc
     2461 tctcggcgtt gaggacttca aggtaaacat aattgaaaag gaagatatca cagttcttga
     2521 tatagtcggc gaaaagctcg gtatcgttat cggcagaaga ggcgaaacac tcgactcttt
     2581 acagtacctt gcaattcttg caagcaaccg tcacgatgag ccttactgcc gtatcacagt
     2641 tgattgcaac ggttacaggg ataagagaga ggaaacgctc aagtcgcttg cagagcgtac
     2701 cgctaataag gtgctccgtc agggcagacg cattaccctt gaacctatga atccttatnn
     2761 nnnnnnnnca gcaaggtagc tgagatcgag ggcgtataca gcaattctat aggcgaagag
     2821 ccttacagaa aggtcgttat ctcggcttct cagccccaca gagataataa caagagaaac
     2881 gatcgcagag gcggcggaag aagaaacggt aacggcggca accgctcgta caagcagagt
     2941 acaggatttt caacatcgtt tgaaagagag tacaagcgct c
//
LOCUS       DAWAOG010000234         2980 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4282_length_2980_cov_2.539145, whole genome shotgun sequence.
ACCESSION   DAWAOG010000234 DAWAOG010000000
VERSION     DAWAOG010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4282_length_2980_cov_2.539145"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          836 a          688 c          730 g          726 t
ORIGIN      
        1 aaccggtcga gccgacacct gcgagtgcgt ttacgccgag gaactgtccg acgactatcg
       61 tatcgaccat attgtagaac tgctgaaaga gaagtcctaa cagcataggg agcataaatc
      121 cgagtatgag cttcataggt gagccttttg taaggtcttt tactgctgac atataaacat
      181 tttcctcctt ttaccgactt agttttaagg gaatcgtttg cgtaacggcg ggaataaaag
      241 atagtgtgct ataaacgtaa tcaatctgtc gaaaaatctc tatcgttaat aaaaaatgtg
      301 gtttggggtt tcggcaagga cgccgatata gaacgcccaa aggctttgca aggacgcaaa
      361 gcccacaaag cgttcttaaa gggatagaga atttgtgcta tctttataga aaacaacttt
      421 ttctacggtg ttaacggctt ttcaatatca gccgattgct aagcaattat actatattgc
      481 ccgtcctgtt tcaatcggta aaatgcacaa ggtatttaag gttatcaagg cggtttagtc
      541 tattgtgcaa cgctgtatcg ctttatttca taacgggcta ttcaacacag taatatcgga
      601 ggatttgaaa aaatttacgg aaatttcaga aaaacacttg acaaacggtt ttacagcgga
      661 tataatgaac acagaatatg ttaaacctat gagtgagagt agtagcaccc gtcactgtat
      721 gacagagagt tgccggtcgg tgtaaaggca atgatacata cgcttgtgaa tggactcacg
      781 agggcaaacc gagagtatca gtttatgata ttttaggggc gacggacacc tccgttacag
      841 gtcgcacata tgatagtatg tgtagcgagt gaccgtgttt tattatacgg taatttgggt
      901 ggtaccgcag gtaaattata ctcctgtccc aactgggacg ggagtttttt gttttcccgg
      961 atactcattc tacgttgaaa ctatcatcat ggcatatggc gtgacagcag ggcatagccg
     1021 acataatgcg ttgctgaata agatacaggc tgtcgggcgc ggactgttcc gcgaaaactt
     1081 aagcactccg ccaaaaaggc ttttgggaaa acaaggcgac acggcaaagc ataacgttca
     1141 gagcgcataa tgttttgtta tcctcattat gcggattatt tcataacaat aaaacataac
     1201 gttcagagcg cagaatgttt aaccccgccg tcacgctaac agtcccatta gcagaaagta
     1261 tattaaaggc ggaatttgaa gcggcacatt gccgccggaa tttgaagcgg ctcacagccg
     1321 ccgcgtagaa aggaaaatta ttatggcaaa cagagagatc ccgtacaaaa tttatcttga
     1381 agaaaacgag cttccccgtc agtggtacaa cgtaagagcg gatatgaaga acaagcctgc
     1441 tccgctgtta aaccctgcaa cacatcagcc ctgtacgctc gaggagctga gccacgtatt
     1501 ctgtacagag cttgcaaagc aggagcttga cgatacaact ccctacatcg acattcccca
     1561 ggagataatc gacttctaca agatgtacag acctgctccc ttagtaagag catattgtct
     1621 tgaaaaggca ctcggcacac ccgcaaagat ctactacaaa ttcgagggca acaacacaag
     1681 cggttcgcac aagctgaaca gtgcgatagc tcaggcttat tacgccaaga agcagggcct
     1741 gaagggtgtt acaacagaaa caggtgcagg tcagtggggc acagcgcttt caatggcttg
     1801 tgcatatctc ggacttgact gcaaggtatt tatggttaaa tgctcatacg agcagaagcc
     1861 tttcagacgt gaggttatga gaacctacgg cgcaaacgtt actccctccc cctcaatgga
     1921 aacagaggta ggaaagaaga taaacgcaga atttcccggc acgacaggct cgctcggctg
     1981 tgcgatttcc gaagcagttg aatgtgcaac aacgcacgag ggctacagat atgttctcgg
     2041 ctcggtgctg tcgcaggtac ttctgcacca gacggtcatc ggtcttgaaa caaagacagc
     2101 ctgcgaaaag tacggcataa agcccgacat gatcatcggc tgtgcaggcg gcggatctaa
     2161 cctcggcggt cttatctctc cctttatggg cgaaaagctc cgtggcgaag ccgactatga
     2221 attcatcgcc gttgagcctg cgtcctgccc ctcgctcaca agaggcgttt atgcttacga
     2281 cttctgcgac acaggcgcag tttgcccgct cgctaagatg tacacgctcg gcagtacgtt
     2341 cattccctcc gcaaaccacg caggcggtct gcgctaccac ggcatgagca gtgttctttc
     2401 acagctctac cacgacggat atatcacagc acgctcagtt gagcagacaa gcgtattcgc
     2461 cgccgccgaa cagttcgcaa gaacagaggg tatcctcccc gctcccgaga gcagccacgc
     2521 aatcagagtt gcgatagacg aggctatgaa gtgcaaggaa acaggcgaag aaaagactat
     2581 cctcttcggt cttacaggca caggctactt tgatatggtc gcataccaga agttcaacga
     2641 ccacgaaatg agcgactaca ttcccacaga cgaggagctt aaggtcagca tggaccgtat
     2701 gcctaaggtt gactgagtgt ataacactat tgtccttgat aaaaattgat atatgaaaac
     2761 gccgccggga gtgatattcc ggcggtgttt cttatttggt cggtatttta gtctttcagg
     2821 gcggtgattg ggacgacgta tacgccgtca gggcgcttgt aggcggcgtt tgacaggccg
     2881 catacgacgc ataacgcttt cggtgctctg ccattttcac tttttatggc attgcttatt
     2941 gatataagat tttcggcggc ggcatctatc tgatttgcgc
//
LOCUS       DAWAOG010000235         2966 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4304_length_2966_cov_2.194091, whole genome shotgun sequence.
ACCESSION   DAWAOG010000235 DAWAOG010000000
VERSION     DAWAOG010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4304_length_2966_cov_2.194091"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2462..2471
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          617 a          784 c          660 g          895 t
ORIGIN      
        1 cttgtctatt attataagct cctcgccgac ctcgcataat atataagtcc ttaaaaattc
       61 gccgatcaca cggatgttga ctttttcggg tgtcggttca ttgatctttt caacccgcac
      121 cggctgagta tttatgaatt tgaagccgtc gtcctttatg ggaggagctt cacgccgtgg
      181 gatatcatcg gtgctttccg cacagatgct gccgatatca tcatcgtcca ccgctactac
      241 taccttgccg cttacagccg tgaatgcggg agctgtcggc ggttcttcgg gaagcgggat
      301 atcatatgct tcctgaggtt tttcagccct tgagatataa tccgtgtgac tgctgtgaag
      361 cgacacggat ctgacctgcg cagagccgta tattgttttt gcttttacag gctcggaaat
      421 ttcttccgga tttgccgttt ccgccgggca ttcgttgttc accggcgcag gtgttaccgg
      481 gatatgcgga agtgtctgct gttcacgctt tattatctca ggattgctct tgaaatagtc
      541 gcccgcattg tcgtatttct tcggtttttc aagctctatc tgtcgtctgt cgtccgcggc
      601 gtttacaagc gcgtttttaa ctgcgaaata caccgcgtca aataccggct tttcatacgc
      661 aaagcgtatc tcggtctttg ccgggtgcac attgacatca accgtatccg gtgcgagtgt
      721 tatctcaagt atgcaggcag gaaattttcc gaccatcata ctgttgcggt aagcctcttc
      781 aagcgccgcc atacaggtgg tgctctttat gtatcttgtg ttgacaaaga acgtctgcat
      841 acttctgctg ttgcggcaga aaagcggcga agaagcatag ccgctgacat ctatgccgtt
      901 aagtgaaaaa tcgaccggga taaggctgtc ggcaaactgt ctgccgaaca ccgcctttgc
      961 cgccgataag agcgagccgt cacccgatgt cattctgacg ctcttgttat ctcttatgaa
     1021 cttaaaggag atatcggggt gggacagtgc aagcctgtca accgttgccg ctataaagtt
     1081 accctcggat atgtcttttt tcagaaattt aagtcgtgca ggagtgttat agaaaagctc
     1141 ctttatcatg atcgttgttc cgacaggaca accgcagcat tcgtgttctt tttcctcact
     1201 gccctcaata acgtatcgac cgccaagctc gtcctctgcc ctgcgcgaca tcatctctat
     1261 cttagccact gccgcaacag acgctaacgc ttcaccgcga aagcccattg tcattatacc
     1321 tgtaagatcg tcgcctgtgc gtatcttgct tgttgcatgg cgcagaaagg ctgtcggcat
     1381 ctgctcatat gctatgccgc acccgtcgtc cgttatacgc atatagctga taccgccgtg
     1441 ttttatctca acggtgatat gccgcgcacc tgcgtctatt gaattttcca caagctcctt
     1501 tattacggag gacgggcgtt cgatgacctc tcctgccgca ataagctccg ctacgctttt
     1561 atcaagcagt cttatctcgg gcattgctct tccttttctt tataaaatta taacattgct
     1621 ttgagttctt cgagctttgc gtaagcctct ctcggggtca tatcgtcaag atcgagattt
     1681 tttatctcgg ttatgacgtt ctgcctgcct gtcgcctcaa agtctatctg gctttcggac
     1741 tcctcctcgg ctctcagctc aagctcacgc tgtatcttcg gtgctctttc aagctcggct
     1801 aaaagctgtt ttgctctgtc ggtgaccttc ttcggaagtc cggcaagacg tgcgacctct
     1861 ataccgtagc tgtcgtccgt tccgccctcg actatcttat gcaggaactt tatatcctca
     1921 ccgctgcgct ttacctttac gctgtagtta cgcacaccgt caagctcgtt ttcaagcgaa
     1981 ataagctcgt gatagtgcgt cgcgaaaagc gttttacagc cgatagcctt tgagcttatg
     2041 tactcggcta ccgcctttgc tattgataca ccgtcatagg tcgatgttcc tctgccgatc
     2101 tcgtcaagga taacaagcga tcgttttgtc gcttcattaa ggatagttgc gacctcagtc
     2161 atttcaacca taaaggtcga ctgacccgcg gacagatcat cacttgcacc gacgcgggtg
     2221 aatatcctgt caacgacacc gagccttgca tagcttgccg gtacaaagca acctatctgt
     2281 gccataagca ctatcacggc tacctgtcgc ataaacgtgg atttacccga catattaggt
     2341 cctgtgatta tcatcaggcg gttgctttta acatcaaggt aaacgtcgtt cggggtgaaa
     2401 agtatctcgt tgaccatctt ctcgacaacg gggtgtcttc cgtcctttat ctctattatg
     2461 cnnnnnnnnn ngtgcgataa aatcgcgcac ctcggcgaat attgccgctt caagtgcaag
     2521 tatcttatcg tttgcgccga gtatctcgtt ttctatcttc ttaagttcat cggtgatgta
     2581 tctctcgccg tttgtgagcg tctgctttct tacatatctg tcgggcacca tcgacacgtt
     2641 gccctttgat acctctatat aatagccgaa cacacggtta tagcctacac gcagattttt
     2701 tatgcctgtg gcttcttttt cccgctgttc tatctgtgca agcaagtcct tacctccgcc
     2761 cgtaatattt ctcaggcggt caagctcctc gttgaagccg ctctttatat atccgccgtc
     2821 cttcataagt gcgggcggtt catctgatat cgccctttcg actaaatctg caatatcatc
     2881 aagcgggctt atcttcttat caaggctctt tgtaagctgc gtgcttatct gtgacaggtc
     2941 gcttttaagc tgaggaagta tcatgc
//
LOCUS       DAWAOG010000236         2960 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4316_length_2960_cov_1.909811, whole genome shotgun sequence.
ACCESSION   DAWAOG010000236 DAWAOG010000000
VERSION     DAWAOG010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4316_length_2960_cov_1.909811"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          696 a          789 c          641 g          834 t
ORIGIN      
        1 taagccgtat ccgtcatatg ctttgctccc ttgccgatat cgtcaccgaa ctgcctgaac
       61 aatgcggaag agcgcttaag actgagcgtg tgcaccgtga tattctcggg agcaagctca
      121 cacagccttt cgagcgagcc ttcaaagctg ctttcggttt ctgtaggaag tcccgcgata
      181 atgtcggaat ttatacagtc aaaaccgact ttccgcgcaa catcaaacgc ctcgcacacc
      241 tgctttacag tatgtcgtct gccaaccgcc gccagtacac cgtcattcat cgactgcgga
      301 tttattgata ttctgcggac accgccgttt ttgagtgccc tgagcttatc ctctgttatg
      361 gtatcgggtc tgcccgcttc ggctgtgaac tcgcggatat tcgcaatatc aagcttaccg
      421 agcttatcaa taaggcggtc gagctgagat gctgacagcg ttgtcggcgt accgccgccg
      481 atatatactg tatcgaccgt gagcgagtac ctgcggacga tatcctcata gatttcaagc
      541 tctttcagca gaagctcaaa ataatcatca acaagcttaa gtgcaccctc acccgatgcc
      601 gaaacaaacg agcaataact gcaccttgac ggacagaaag gtatcgatat ataaaggctt
      661 atcctattca gtgtcagcgt atcaagtatc ggcttttgca gacgtataac atcattcgca
      721 agcttcagct tcttatcgct cacaagcagg gtgtcatgga tatagtcatc tgcattgtcg
      781 cgggtttcgt atatattgcg gatatacttt acaggacgga ctcctgtgag tattccccac
      841 gggagcggcg cgtgacctag cttttccata catctgcaaa gaagtctgca cagctgggtt
      901 tcggcatcat cggtgtagct gtttgttctt gccgtcactg tcttgccgct gtcagaaaca
      961 gtcaccgaca gcctgccgcc gtcagctgtg aaatatgcat agtccgtacc tgagggtgct
     1021 ttgccggtat aaaaatcaag cttgtgcggc aaagagaaat ttcttatcag ccgctcaagc
     1081 tcataggcat agtcatatgc cgtgctgtca aaatacaacg aatttatcat atcagaaaaa
     1141 gtccacagag ctttgtccga ggaacggatt gaactgcttt tcctgcttta atgtagtagt
     1201 tcctccgtga cccgggtaaa tcagataatc ctcgcgcagt gccgcaagcc tcgcaaggct
     1261 gttgcgctgt gcaatgtaat ctcccgaata gaagtcggtt ctgcctgccg agccttcaaa
     1321 tatcgtatca cccgcaaaca tacagttatc cgcaaagaaa accacactgc ctgccgtatg
     1381 tcccggagtg ctcataacgc tgaattctac ctcgtcaagg cgtattatat caccgcccga
     1441 aagcaaaaca tctgccgtaa acggtttata tgaataatca ggacaaagat acgcaacatt
     1501 ctttattgtg tcatcaagca tacattcgtc ctttgcgtgt atgtacacct ttgcgcctgt
     1561 ctgttctttc agatctgcaa cagctccggt atgatcaaaa tgaccatgcg taagcaatat
     1621 catcttcagc tccgcatttt ccgcactcag cgtatccagt atacgctgtg cattatcggc
     1681 aggatcgatc acagcggcat ttcccgcggc gctcgttacg atatagcaat ttgttccaag
     1741 ttctcctaaa gtaagcgtct ttacttccat tactgtttac ctgtcctttc ggcagagatc
     1801 actcccggta tcttctgaag acgcagtata atgctcttca gctggtcaat accgctgact
     1861 tctatagtaa gaattatatt tgcgttgccg tttttaagat agtgcgcgtt gatctcgtgt
     1921 ataggcagat gattacttgc aatagccgtt gtgacatctg caataagcgc cgttctgtca
     1981 tctgcgatga tatcaacagt cgctctgaac gcactgcttg aatttaccgc ccagtgagcc
     2041 ttgacccatc tgtccttatt ttcgccctgc ataccgagct tgacattctg acagtctgcc
     2101 ttatggatag acacgccata acctcgggtg ataaagccca taatatcgtc gccgggaagc
     2161 ggattacagc actgcgcata ctttaccgca cagtcgtcga taccgtcaag aacgactccg
     2221 ttcttctgcg ctctcttgtt gttgtcggct atagtctttt cgatatcgat aggctgcgcc
     2281 tgagccgcgt taaaacgctg ctgctttatc tgttcttcct taaggcgcgt gattatcttt
     2341 gtaagctgta cgccaccgta gcctatagcc gcatacagat catcgaccgt tgataggcgc
     2401 tgacggtgag ccgcctgtaa aagaatatca tcgtcaacag taatgttgtt tctgcggatc
     2461 tccttttcaa atgccgcctt accgcattct atattctctt ctctgcgctc acgcttgaac
     2521 cacgctctta ttttcgcttt tgcttcgttt gtctttgcta tctcgctcca gttgcggtta
     2581 ggaccgtaat tatcgctgcc gcttgtcagt atctcaacga tatcgcctgt gtggagcgta
     2641 tggtcaaacg gcaccattct gccgccgacc tttgcgcctg tcattttatt gccgacctgc
     2701 gtatggatag cataagcaaa gtctattaca tttgagcctg ccggcagtgt tataacatca
     2761 cccttaggag tgaatacata tacatcatcg ggagcaagat cgacctttat tgcctctgat
     2821 atctgctcga catcatcagc ctcctgctgc tgctcaagca gctgccttat ccagtcgaaa
     2881 cgcttttcac cggcaacatt tcccgatatt cccgctttat acttccagtg cgccgcaaca
     2941 ccgtactgag cggtattgtg
//
LOCUS       DAWAOG010000237         2946 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4348_length_2946_cov_1.879972, whole genome shotgun sequence.
ACCESSION   DAWAOG010000237 DAWAOG010000000
VERSION     DAWAOG010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4348_length_2946_cov_1.879972"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          745 a          653 c          615 g          933 t
ORIGIN      
        1 ggcataaagc ttgccggtat cttccttttt tatatagctt tcgataagct ttgagtctgc
       61 gtatgtaaga agttcgcgca gatagtccgc aagtgtcttg tcatgcgtga gcagatatcg
      121 agctatattt gtatatgcgt ctatcgctgt gcattcatca tcgcttgcga gctgttcaaa
      181 ctgtccgata agataatctc cgctaatgtc aaatgcggta agagatctta atttgtgtgc
      241 tgtttctttc attgttttgc ctttcctttt cgtcctttat atatcctgct gtgccgtgtt
      301 tcttataagc tccagcatat tgtcaaaatc ttcgagcgtt gacaggctgc cgcacatatt
      361 gcgcagcttt gccgcccccg gtctgccttt gagataccag gcggtatgct tgcgtgcttc
      421 cttcattccg atatattcgc ctttatcggc gcagagcagt cttatatgct cattcatgat
      481 atcaagctgt tcatcggggg agggcgcagg taatgtttct ccggtttcaa ggtaatgctt
      541 tatctcggag aatatccatg gtctgccgca ggcggctctg cctaccatca caaggtcaca
      601 gcctgtgtaa tcgtacatat gcttacagct ttcgccgctg tcgacatctc cgtttcctat
      661 tacaggtata ctcagcgcct gttttacctc tgcaattata tcaaggtcag cctttccgct
      721 gtacatctga ttttttgtcc tgccatgaac ggcgaccgcc gatgcaccac tttcctgtgc
      781 cgcacgggcg acctcaaccg catttacact attttcgtcc cagccttttc ttattttgac
      841 agtcacggga atatctgttg cttttacgac agcctcaaca atttgtccca gaagaacggg
      901 atttttcatc agtgccgaac ctgcaccgtt tcccgcaact ttcggaacgg gacagccgca
      961 gtttatatct atgaagtcgg gagcatactg tgccgctatc actgcagctt tagccataaa
     1021 ttccggctcg ctgccgaaaa gctgtattgc cattggacgt tcacagtcgg ttaccgtgag
     1081 cagacttgca gttttgcggt ctgaataaca aagccccttt gcactcgcca tttcccctgt
     1141 gagcagtgcc gcaccgtatt ttttgcacat agtgcgcatt gctttgtcgg caaccgatgc
     1201 cataggggca agcgatgctg ttttttctat cctgacattt cctatcgtag tgtattccac
     1261 aatctgtaat aagcgactcc tttccgaata tatttaaata attatatcac tttttgcgcg
     1321 tctgttcaat actctacaac gctctgaaga cagcggatgc tgtattttgc agagttttta
     1381 tacaatattc ctatttatac cttgcaatcc gacaaaaaat attgtataat attacacggc
     1441 gtttttactg caaaatgatt tttgtttatt taaacgctgt taacattcgt atgttatcat
     1501 gcgcataatg ccgcaaggct ggatttgttc tttttaagga gttgttatct tggcattcaa
     1561 gttatttgat tatcagagtt cgggtaaagg tgtatcaaaa acagcaccgc agaaaaagcc
     1621 gtttttcaga tattgggaaa tatttggcag aaagttttgg aaaatcatcg aactgaattt
     1681 aatttatgtg cttttctgta tacctgttgt gacattcgga ccggcaacgg cggctcttac
     1741 tcatgtaatg agaaagttta tacttgaaca gccttgtttt gtgtttgacg agttttttac
     1801 agcattcaaa aagaatttca agcagtcggt atttatcggt attgtcgatg taattatgtt
     1861 ggtgtcactt tttatcgcac tgtttcagta catgtcgctt gataagctgc ctgacggcgc
     1921 tattgtgtat atctgtatta tgatctgctg tgcaacactg gttttcatga tgcacttcta
     1981 catatatctt gagatagttg cactcaaact ttcgctcaaa gctatactca aaaacgcagt
     2041 cttccttgtg ttcctcggag taaagcgtaa ttttattgcc ctgatagtaa acctgacgat
     2101 aattacgctt gttgttttat tccttcctta ttcggttttt gtggttatag tattcccgct
     2161 ggcgtttatg agctttacta caactttcat ttgttatccc ataatccaga aatacatcat
     2221 caacccgtat tatgaggaac gcggcgagca caatcccgag cttggcatca ctgaagatga
     2281 tgttgcagaa aacgctttat tcgttgacag aggcggaagc gaaaaggaaa tcaaagcatc
     2341 gcccaaggct cacggaaaag ttattaaata attattacgg cattcccgca gaaagtgtgg
     2401 gaatgccgtt ttgcgtaaat tatgcgcatt cgccgcgtat ttacaaaaac tgttgcatta
     2461 taccttaaaa agtggtataa ttattttaag tatcgtgcag tttttactgc atcgctatcc
     2521 ggcagaacaa aaagaaagaa gggtattgat atgaaagcag atttacattg tcacactaca
     2581 atgtcagacg gctcactggg tcttgaggaa accattgcac aggcaaagcg atacggcatc
     2641 gattattttg cgataaccga ccatgacacg ctatcttcct cgtcaagggc tgtggtactc
     2701 ggacagcgtt atggtgtcaa cgtgatccct gctgttgagt tttctacata cgacagcaaa
     2761 cgaggatcca aagcacacat aatatgctat aatcccgaaa agccaaacag acttgaaggc
     2821 ttatgcctgc gcacttgtga actgcgtaag aagcgcggca aacagatggc tatgaaagta
     2881 cttgaaaagt accccataac gcttgaaagc atacttcgtt acgccacggg cagtaaagta
     2941 atattc
//
LOCUS       DAWAOG010000238         2939 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4358_length_2939_cov_1.995492, whole genome shotgun sequence.
ACCESSION   DAWAOG010000238 DAWAOG010000000
VERSION     DAWAOG010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4358_length_2939_cov_1.995492"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2173..2182
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          653 a          749 c          680 g          847 t
ORIGIN      
        1 ctgccactct ttttccattg cctttaatgc ggactgaatt tcggtgcgtc tgccctgctc
       61 cttatatgtc tttataagct ctgctttctt ttcaaaaagc tcgcccttac cggacgcaag
      121 ctcaattgca aggttctgat aatactcagc tacctgcttg cctaagttac ggctgcccga
      181 atggataaca aggtacttat taccgtccga ggaaacgtct atctcgataa agtggtttcc
      241 gccgccaagt gtaccgaggc ttttttcaag ccgttttgcc tgttcgagat gtctgtagca
      301 acgaagtccc gtaaggtcaa agcgttcata acgtccgtcc catacgtttc ttccgctcgg
      361 tatgtagtgt gcggcttcat ctatctcact aaggtcgata tccgcctttc cgagctctgc
      421 tgtgtacatt ccgcaaccga tatccacacc tacgacattg ggtactgcct tatcggttat
      481 cgtcattgtc gtgccgatag tgcagccctt gcctgcgtgt acatcgggca ttattctgat
      541 ttttgagcct gcggtgaact catagtcgca cattctgcgt atctgttcta ttgcttcgtc
      601 ctcgactact tttgcgtaac atattgcagt gttgattttc cctttgattt caagcatatc
      661 tctgctctcc tttctcaaaa aaataagaac aacgtccctt aaaaaatatg atgggaatag
      721 gcagggacgc tgtatgttaa caagacgcaa taaataaaaa tgtacggact cgcaccgcac
      781 ggtagcctgc cagaaagcaa gtgccgttag gtcggcaaaa aagtgtttaa aaaacacagg
      841 tgtaaaatgc tgctgtatgc gtctttacaa ggcacggtta ttttccatat ataaataata
      901 tgaactgctg tctgtgcctt gaacactctg caaggcaccg tcttaatacc catatagaaa
      961 ataactgctg tcggtgcctt ttattaaatt gtacaagggg catcagccct ttgtctgctg
     1021 tgaagctttg cttcatcgca tgagtttatt atacaggata ggatttggtt tttcacggaa
     1081 aaaaagctgc gaactttatt tcggttcaca gctttaacag attatcgaaa tttaaacgag
     1141 cttctgctgc ggcgcaaagc tctgtcgccg gcggggaaag agcgtttgtg tctgtccttg
     1201 ccgccgtccg tgccggggct gagtgtgagg atacaccctc ccaagtcgcc tttttaaaac
     1261 gtataggata gtataaaccg cccgcggaac attccgtgag cggttttgca gtattcttat
     1321 attgtcagtt tgcgacaatg tttacaagct tgccgggaac aacgatttcc ttacggatcg
     1381 tcttgccctc gagcgacttt gcaatagctt cctttgccat cttcagcagt tcgtccttgt
     1441 cggcggctgt aggcacgttt atacgagcct ttatcttgcc gcagagctga acaactatct
     1501 caacggtgct gtcaacacaa agcgcctcgt cgtatgttac ccactgcgct tcgttcagta
     1561 tgccgtagcc gagcgagttg tacatttcct cggtgatgtg gggagcgaag gggttaagga
     1621 ttatcagcag cgacttcatc tcggcatcgt tgatcctgcc gtttgcagtg atctcgttga
     1681 taagcgacat catagcggcg atagccgtgt taaatctcat cttctcaata tcttcgctga
     1741 ccttctttat cgtcttgtgc attgatgttc tcagctcgtc agagtattca tcgccgcgta
     1801 caagtatatt ctgaagattc catacacggt caaggaaacg cttacagccc ttcatactgc
     1861 tgtcgttcca gggagctgcc tgctcatagt cgcccatgaa caggacatat acacgcagaa
     1921 cgtctgcgcc gtatacgtca actacatcgt tcgggtcgat tacgttgcct cttgacttgc
     1981 tcatcttctc gccgtccgga cctaagataa ggccctgtgc ggtacgcttt gcgtaaggct
     2041 cgggagtggg aacttcgccg atatcgtaaa ggaacttgtg ccagaagcgc gagtagatca
     2101 tatggcgggt aacgtgttcc ataccgccgt tgtaccagtc aacaggaagc cagtatttga
     2161 gcgcttcctg agnnnnnnnn nnaagtacca gcttgagcct gcccactgag gcattgtatc
     2221 tgtttctctg cgcgcgtcct tgccgcactt ggggcacttg cagtgtacga atgaatctat
     2281 ctttgcaagc gggctttcgc cgtcctgacc cggctggaag ttctcaacgg gaggaagctc
     2341 cagcggcagc tcgtcataag gaacgggaac aattccgcag tcggggcagt gtactatcgg
     2401 gataggctcg ccccagtatc tctgacggtt gaacgcccag tccttcatct tgtactgtac
     2461 gcccttttcg ccgcagccga gctttgcaag tacgccgagc atcttctcaa tagcatcctt
     2521 cttgttggtg ataccgttca actcacccga atttaccatc tcgccatcgc ccgtgtaggc
     2581 ttccttggag atgtcgccgc ccttgattac ttcgataata tctatgccga acttctttgc
     2641 aaattcatag tcacgggtgt catgagcggg aactgccata atagcgcccg tgccgtatcc
     2701 catcattaca tagtcggaaa tgtatatcgg gatctccttg ccggtaacgg ggtttacgcc
     2761 ggtaagaccg tctatcctta cgcctgtctt atccttgaca agctgtgttc tctcaaactc
     2821 gctcttctta ctgcattcag ccttgtaagc gtcaaggtcg gcgatgttct ttatagaatt
     2881 tctgtacttc tcgattatgg ggtgctcggg ggcgatgacc ataaaggtta caccgtaga
//
LOCUS       DAWAOG010000239         2938 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4360_length_2938_cov_1.808880, whole genome shotgun sequence.
ACCESSION   DAWAOG010000239 DAWAOG010000000
VERSION     DAWAOG010000239.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4360_length_2938_cov_1.808880"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1337..1346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2170..2179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          725 a          630 c          597 g          966 t
ORIGIN      
        1 caataccgtt ggtattttcg tcctgcatcg agagagtgaa gaaccttacc ctttcatcac
       61 cggtttgtgc cgaatactgc tcacatacgt tctttatgtt tttcagaaga gagtttccca
      121 tgacaccgta tgtgcagaat atatatgcat cgggattatg agaacgtacc agcttgagaa
      181 actcgatata gccttcttca aattccgctc tggtcgcatc atttgttgca tagttcatat
      241 cgtttgtgcc aaggtttatt accactatgt ccggcttgta attgttaaag tcccattcga
      301 gagaggcaac ttcaaggtta tctgcaaact tgttgtatga gaagccgagt ttgtcatagt
      361 actgaggtat agtctgttcg ggctttttct tgccgtcatc ggtataaccc gaaataatac
      421 cgtaaccgct tatcgatacc atgctgtagt ccgcattcag cgcctttgcg gttttatacg
      481 cgtatgcctt tgttacgtct tccgttgaag ttttgaaatg atgttctttg ttttcgtcat
      541 caacgccgta accgcaggta atagagtcgc cgatgaactc catcttaaga tcttttgcct
      601 ttgcgggttc gatcttctca tcgtctgcga tttttatcgg ctttatgccg aatgttgaca
      661 ttgcacattc ggagagcttg attatctgaa catcaacgct ttttgctgta tcgctttcaa
      721 atgctgtata cgtcttttcc ttttcgttca gcatatcatc aacaacacgt tcaccgtcaa
      781 tatatatcgc aacacgggca tagttttcgt cattgcctgc tgttgaggcg taatctccta
      841 tgactgtgat gtcaagtttt tttccggtgt atgtaaattc gacacctgaa ccggaaaatg
      901 ctgtccatag tgtatcatca ttcagatacg ttctgccgac aagctttgct gtatcggctg
      961 tcagtactac tgtctgaccc tcctgttttt ccgcagatga tacttctgtc gaagacactg
     1021 cagagctttc tgccgttaat attgcactat tcatgttttc ctccgttttg ctgtcggtgc
     1081 agcctgatat tgtaccgacc agaattgatg ttgcgcaaag tgcgcaaatg atttttttgt
     1141 tcattttctt tcctttcatt ggtttttgct gaaaaacagc atttttctta taatgttaca
     1201 gctctccatt ataaaaaaat acggctgtaa aatcattttt cttttctgac agacaaagcc
     1261 aaagcctttg tttcttcctt gcttaaccca tcggcagaca actcaaatat ataatctccg
     1321 ttgtcccata tcaggtnnnn nnnnnnacat aatacattgc ggtgtatttg cctagcggcg
     1381 ttttttcagg aaaaacctgg acgttgccaa aacctatctg agttgaattt gcatagtttt
     1441 tggtgacctg actgaaccag attgttttgt cctcttcgca ggtgtatact gcccagtagt
     1501 aaaactcgct gtcgtttata atttcttttt tatattcgct gagatctgtc gtaatcatat
     1561 atttttcggt cagctgtaag ggtgcactat tgggatcgtc aaccagaaac ctgtattcat
     1621 cgttgccgac atttttaatc ttaaaagtat cccagtactg tagcagtacc gcacttcccg
     1681 taccaagtgc gacggtagcg gcgataattg ccgcaataag caggcgtatt ttaattctgc
     1741 gtctgtttat tatactgtct ttattttttt ctttgaaata ggattgtatg agcttttgca
     1801 tacgtctttc aaagctgtga cttatcttac agtcaggcag tgcttcaaat accgaaagct
     1861 cggagagata gtcgctttta agggcggcgg ctatcttttc gtcgtttgtc ataatgatta
     1921 gtccttttgc atcagcagag tttttcgtat ccttgacaga taaacgtgca cggtattcgg
     1981 ctcgatatcg agcatttcgg caatatcatc ggtaggaagc tgttgtacat agtacagaaa
     2041 agcaatattt ttcagctgcg gcgataacag tttcatattt ttgatgatct tctcataagt
     2101 tatatcaact tctgcctgct gcgctaaatc atcttcaaat atcttttcgt ccaaacagac
     2161 tttttgcatn nnnnnnnnnt gttttttgta ataacggtaa tatatgattt tgtttttcgc
     2221 gaatatgggt tttgtatctt gtcaagattt ttgattatcc ttgcaaatga gtcctggact
     2281 gcgtcctccg catcttcctt attgccgagc agggacatac ttatattgta cataaactgc
     2341 ttatatgcgt tatatagttg tgctattagt tctttttcgg tcggcgtatc gaccatagaa
     2401 atgtaagcgg aaagcaaatt gatcctccgt gaaccgtttt atataatgta ttctaacaca
     2461 ggaaaagcca aaagtcaaac gatatcggta taacagcgtt caataagaca gccctgagaa
     2521 aatccgaacc gatgtcttta gcaacgaccg ataatacgga acgtttattt tgtatgtctg
     2581 ttaatatact tgctgtgaag ctttgagtac attatcttct gaccggtgta aagtccgtag
     2641 tagcctgata ggatatagct taccgagcag gcggcggcaa agaagataat cccctctcct
     2701 ccgaacagct cgatgctcag aagaagcgag gtcacagggc agtttaccac tccgcagaac
     2761 agcgaaacaa gaccgagtgc ggcggtgaac tgcgggggca gtccgaacag cgttccgaac
     2821 agattgccga atgtcgcgcc tataaagaat gacggtacta tttcgccgcc cttgtaaccg
     2881 cccgcaatgg ttaccacagt gaatacaagc ttcagtgcaa atgcctccgg gcgtgtag
//
LOCUS       DAWAOG010000240         2937 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4361_length_2937_cov_2.626301, whole genome shotgun sequence.
ACCESSION   DAWAOG010000240 DAWAOG010000000
VERSION     DAWAOG010000240.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4361_length_2937_cov_2.626301"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          812 a          725 c          655 g          745 t
ORIGIN      
        1 gttacaatga tacccgcgtc acagccaagc gctcttaccg cccatgaaag cataggagta
       61 ggcataagct ctgtgtagat gttcgctgta atgccgtttg cggcaaagat agaagcggcg
      121 tgctttgcga atacgtcaga ctttattctg ctgtcgtatg caactgcaac cttaaggtcg
      181 ctcttgcctg tgttcttaag gtagtttgca agaccctgag tagccttacc tacagtataa
      241 acgttcattc tgtttgtacc tgcgccgata acgcctctca gtccgcctgt tccgaactcc
      301 agatcacggt agaaacgatc atttatctca tcgtccttgc cctttacgct ttcaagctct
      361 gtctttaaat cgggatcctt aaccgctttt tcaagccact cattgtaaag ctctgtaatg
      421 tttgccatgg tagtctgtcc tttctgtata aaacggcagt catagcagca tagcttttaa
      481 gctgaatata gctatggcta acctaaattt ccattcacta gacagtataa catttttccg
      541 ccgtaaatgc aaataaaatc acaaaaattt tattagattt ttcggcagta aaaaaactcg
      601 tttttgttga aaatatacct aaaaggttgt ataattatta catagttatc acttaaaatc
      661 agatgaaaaa ggtgaaaata tgtattctca ggtcaacggc atgggacttt tcggcatgaa
      721 tgcatttaaa gtcacggcag aaataatatc atcaagaggt cagccgtcct tcgatatagt
      781 aggactgggc gatcttgccg tgcaggaaag caaaatgcgc ataaagggcg ctcttgcggg
      841 catcggcata acgataagcg gtcagaagct gacggtgaat ttagcgccgg cagacgtgcg
      901 caaatgcgga tcactgtatg atttcacgat aatcgccgct atactggcag taaataacat
      961 catcaccgat gacctgtcag actgcgcctt tataggagag gtttcgctcg gcggctcgct
     1021 tgtgttcacc ggcggagtta tcagtatggc tatagaagct aaaaaatgcg gcataaagcg
     1081 gctgttcctc cccgccgaaa acgctaagga ggcgtccgtt gtcgagggtc tgtcagtata
     1141 cggcataagc cacatttccg accttataaa tcatttcaca ggcaaaaagc gcatacaacc
     1201 cgagccgccc tacgttccct cccccgagat gtttgaaaca gacgacttat ccgatgtaaa
     1261 gggtcaggcg cttgcacgtc acgctctgga ggtagccgcc gcaggcttcc acaacgtact
     1321 tctcataggt cctcccggca cgggaaaaag tatgatagca aagcgcatac cgtcaatact
     1381 cccgcccatg agctttgatg aaagcattga aacaacagga atacattcta tagccggtat
     1441 gctcgaccgt gaaaagccga tagtcaccgt gcgccctttc aggagcgtat cacacactgc
     1501 ctcggcagta ggtcttatcg gcggcggaag catcccacgc ccgggcgaaa tatcccttgc
     1561 acacaacggc gtattatttc ttgacgaact gccggagttt gaccgcagaa cgctcgaaac
     1621 gctcagacaa cctcttgagg acggaattat aaccatatca agagcgcaag ggtcggtgtc
     1681 atatccgtgc gatattatgc ttgttgccgc aatgaacccc tgcccctgcg gtaatttcgg
     1741 caatccgaaa ggcaaatgca cctgctctca gaatatgata cagaattatc tcggcaagat
     1801 aagccgccct gttcttgaca ggatagatat acaggtggaa atgccgcagt tatcatacga
     1861 ggaaataagc agcgcgaaaa agggcgaacc gagcagtgcc gtgcttgaac gcataatgaa
     1921 agcgcgggaa atacagcaaa agcgctttgc gggtacggat atccgctcaa actccaagat
     1981 cccgccctca tatcttcatg aaatgtgccc gatggacgaa aacgcaaaga agatgctctc
     2041 cgactgcttt gataaaatgg ggctgtcggc aagagcatat gacaggatac ttaaggtcgc
     2101 acgcacctgc gcagatcttg ccggaagcga gataataaga cggcaggata tatcctctgc
     2161 ggtaaatttc agaagccttg acaggaaata ctggggtgcg ggaaaataac cgtcatttta
     2221 ccgcctgtca tatttataca gcgcggtgaa atatcagaca acggagctgt gacacaattc
     2281 ctcagcttat caattgcgct aaaattacaa tgcaacatat tgtaaaaacg ccaagacaat
     2341 aaattacagc caacaccgcc ttcctctctc cccttgggtt gtctccctcg ttcatagcag
     2401 caacgttatc gcacagaata gcgaacccga gaagtgctat cctacaaaga gaactatcgg
     2461 tagcctgcta tcattgttaa ttactaaaat ggtataacag aagtacacta aaaaaacaaa
     2521 ctttctccag atattaaaaa agtgagccga gcgcactatc ctttgcgctt cgactcactt
     2581 tttcttttcc gggttgtatc acaacctgac gcttacatat gtaccgtaaa gcggtgtgct
     2641 tccgctcata tgataagcgc aaaccctgaa cctgtatgat gtgttctttg caagaccggc
     2701 ttttctgaat gtagttgtgc tgttgtcggt tatctttgca acacgcaccc actcaccgtt
     2761 ctgagccatt tcaacaatgt acccctgagc tgaggtgttc ttgctccagt tgatacgcaa
     2821 tgcgtccttg gcagtaccgc ctatcttgac acctgtcatc acagacggat ttgtccttgc
     2881 tgtgacttcg gcgctgtaat tgccgtagta cgctgctgtt ccgctcatct tataagc
//
LOCUS       DAWAOG010000241         2921 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4387_length_2921_cov_2.284020, whole genome shotgun sequence.
ACCESSION   DAWAOG010000241 DAWAOG010000000
VERSION     DAWAOG010000241.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4387_length_2921_cov_2.284020"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1552..1561
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2596..2605
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          863 a          583 c          697 g          758 t
ORIGIN      
        1 gaatagcatc tgttagtaat gtagtgagaa tcttcaaagt gctgaccaca aagttatgtc
       61 atatcaaacc caagattttt caacgctcgt tttacaacat tataatccgc aacgataaag
      121 attatttaag gtgttgtaac tatattgcca ataatccttc taaatgggaa gatgacggtt
      181 tctataacaa aacaaaagca gtagatttcc tctgagataa aaactgtaaa ggtgaaatat
      241 tatgaaaaaa ctcggtgtta tacgactcat cgtctgcatt gcggcgataa taatggaact
      301 tttaccgctc ggtgcggtac tcaaatacgg attgatgtcc gacagcggtc atttgatttt
      361 ccgctttgag aattactcgt atttcgatgt cactccgttc ggatatgcaa tgttcaacta
      421 tatgatatgc gctgtgatga caactattac ggctatgctt tcgcttctgt ggatattctt
      481 cggcaagaag cgtcagacac ctattacggt tttagcgtcg atagcactgg taatgtcgct
      541 cgtcccgtac cttacacagg cattcaatgt ttttactgtc ataatctcag cgcttatagc
      601 ggcggtgctt gcactttcgg taataattca gataaagcac gagtaggaaa ggaaatgtat
      661 tatgtcacgt tcttctaacc aaaaagcgaa gctgttatat ctggaaaaaa tgttccgcga
      721 ggagtcggac gaacagcatc cgctcaccgt aaagaatata atatcaggac ttgacagagt
      781 cggaataaaa gcggagcgta aagccatata tgacgatata gagcttttgc gcgaggtaat
      841 ggatatggat atctgctccg acaaggtagg aagctattat ctcgggactg acaggtttca
      901 gtttgaggag cttcaactgc ttgccgatgc ggcggcttgc tcaaagttta tcaccgagga
      961 taagtcaagg gagctggttg agaaaatatc acatctcacc agcaacttca aggcgcgcga
     1021 gctgcaaagg agcgtgatcg tcgcaaaccg tgttaaaacc gaaaaccgca agatatacta
     1081 taacatcaac aaaatacacg aggcgataaa gcagcagaaa aaaatcacgt tccactactt
     1141 caggtatgac ataaaaaaga aaaaagtcta caagacttca ggcggacggt atgctatgtc
     1201 gccgtattca cttgcgtggg aagatgaaaa ctactattgc ataggctact acgaaaagta
     1261 cgacagtata tcaaacttcc gtgttgaccg tatggaagat gttgaggttt ccgatgagcc
     1321 cgcagttccc gcaaaaggat ttgatcttgc ggaatatacc cgcaggatat tcggtatgtt
     1381 tgcaggaagc gaaaccgtac gggctaaact cagctttgac aattcgctca caagcgttgt
     1441 gatggataag ttcggctcgg atatcactat gcataagctc gatgaaagtc atttttacat
     1501 atcaaaggaa ataaacgtca gcccgacatt tttcggctgg ctgttccagc tnnnnnnnnn
     1561 ntgagatact tgagccgact gcattgagaa aagaatttat cggctatctc gacaaggtaa
     1621 gaaatctgta ctgaaccgat attgagaaag atgtatttgt atggcaagga taagatccgt
     1681 atttacgaaa attccaaagt ggctttatta cacagtgccg gcatttgctg tgtcgctgat
     1741 acttatactg cttgcaaaaa atacgggcgg ctttgccgaa tggtacagta caaatatata
     1801 tccgtttttt gtaggcactg tcgggcggct ctcttcactg ttgccgttct cgctgtgtga
     1861 gatactgctt tacacggtga tcatactcgc cgctgtcggt gcagtattta ccgttgtcag
     1921 atttatcaaa ggcaagggta agcgaaaaca gctgatgatg aacgtgcttg ccgttgtgct
     1981 gaactttgct gtcagcgtgt tctttgtatt tacgcttttc tgcggcataa actacaacag
     2041 aacgccgttt tcggcagtca gcggatttac cgttgaaacc tacacggcgc aggagcttgc
     2101 cgacctatgt gtatatattc ttaaaaacgc aaatgacgcg gcgcagaaga tagaaacaga
     2161 tgaaacgctg acggtagagc ttgacggcaa aatagatctt gacgaagaat gcaaaaaggc
     2221 aatggacctg ctcggcgaaa ggtacggtag cctgtcagga ttttatccga acgcaaaggc
     2281 ggtgatcgcg tccgagggta tgtcatatat gaaaatactc ggaatgtaca caccgtttac
     2341 gatagaagcc aactacaatg tgaatgcgcc ggactttgca aagccgttta cagtatgtca
     2401 tgagctgtcg catttaaagg gctttatgcg tgaggacgag gcgaatttta tcgcttatct
     2461 tgcgtgcaca ggctcggata acatttatct gcaatatgca ggctgggtat atgcttttat
     2521 ctatgccggg aatgcgcttt attcggcggc aggagcagat atctactatg agcttatgag
     2581 ctatgcggac gaaaannnnn nnnnntagca gagctgagag ccgacagagc gtactggaag
     2641 cggtacgaaa cacccgttgc cgcagtcgcg acaagcatca atgatactta tctcaaatcg
     2701 cagggacagt cggacggaaa gaagtcctac ggcaggtttg tcgacctgat gataagctac
     2761 gaaaaaatgc aggcatcgta attcaaatgc cgtcaggtat tataaatatt ggagtaatca
     2821 acttatggca aataagaaat tgtacaagaa agaaagattt acgggatgta tggcaggcgc
     2881 tgccgcaggc gatgcgctcg gctcgcccgt aaaggggata a
//
LOCUS       DAWAOG010000242         2914 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4398_length_2914_cov_2.575376, whole genome shotgun sequence.
ACCESSION   DAWAOG010000242 DAWAOG010000000
VERSION     DAWAOG010000242.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4398_length_2914_cov_2.575376"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          687 a          758 c          619 g          850 t
ORIGIN      
        1 gctttacggc acccgttaca tatgcggcgg cgtgtgcttt agggaacata tactttatct
       61 tcttacagct ttcaatatac cactgaggta cattattctc ttcaaaggcc ttgtatatat
      121 cctcgttgaa cagcttcttg gcattgccct tacgggttat ctccattatc ttgaatgcga
      181 gcttcggctc aagtccctta tgcataagat aaaccataat atcatcacgg caaccgataa
      241 cctcggaaat cgtgcatatt ccgtccgaaa taagctcctg tgcgtttccg agccatacgt
      301 cagtaccgtg cgacagaccc gagatctgta aaaggtcgga gaacttcttg ggctgtgcgt
      361 cttttagcat ctgcaaagtg aacggtgtgc caaattcggg gataccgtat gtaccgcttg
      421 aaacaccgag atcctcctcc tttatgccga gcggctctgt cgatgtgaac agctccatga
      481 cctttgggtc gcttgtcgga acgtccgcta tctttatgcc ggtcatatcc tcaaggtgct
      541 tataaagcgt cggcacatcg tgtccgagct cgtcgagctt aaggatagta tcatgcagtg
      601 catggaagtc gaagtgcgtt gtgatcatat cgctctccgt cttgtccgcc gggtgctgaa
      661 ccgccgtaaa gtcataaacc tcataatcgc tcggcactac gaccattccg ccggggtgct
      721 gagatgttgt tctcttgaca cctgtacagc ctgccgcaag acgtgatatc tctgctgaat
      781 ttgcagtcat acctttcttt tcaagccact tgcgtacaaa gccctcggca gtcttttcct
      841 gtacagctga aatagtaccc gccttgaaaa cgtgatcctt accgaacagt tcttcggtat
      901 atctgtgtac ctttccctgt acctcgcccg agaagttaag gtcaatatca ggcgacttat
      961 cgccgtcaaa gccgaggaac gtttcaaacg gtatatcgtg accgtcacgg atcatatcta
     1021 tatcgcagtt ggggcacttt ttcggcggca ggtcaaagcc cgagccatat gagccgtcaa
     1081 ggataaattc gttgtgtcgg cacttcgggc atctgtagtg cggaggtaag ggattgacct
     1141 ctgaaatacc agccataata gcaactaccg atgagcctac tgagcctcgt gagcctacca
     1201 gatatccgtt cttttcagag aacgcaacaa gcttctgcgc tatcatatac agtacggcaa
     1261 atccgtgctt tatgatagag tcaagctctt ttttcagtcg cttttcaacc gtttccggca
     1321 gatcgtcacc gtaccacgcg tgtgccctgt cccagcaaag ctgctgtaat tcttcgtccg
     1381 caccgtcaat atggggagta tatgtgcctg tgggaatagg ccgtaccacc tcaatcatat
     1441 cggcaattct gttcgtgttt gtaataacga cttcctttgc cttttcttca ccgagataag
     1501 caaattcctc aagcatttca tcggtagttc tgaaataaag cggcggctgg tttgcaaaat
     1561 cgtcatagcc ctgtcctgcc tgcaacacct cacggaatac agcgtcctca gggtctttga
     1621 aatgcacgtc acaggtagcg actaccggct ttccgagcct ttcaccaagc tcgacaattg
     1681 cacggttgag atctctcaga tcctcttcac tgcttacctt accgtttcta agcaaaaaca
     1741 tattattgcc gataggctgt atctcaagat agtcatagaa agatgctatc tcttctatct
     1801 tctcctgcga ctccttttca agtatcgccc tgaaaagctc gcccgcctca cacgcactgc
     1861 ctattatcag tccctctctg tactgcgaca ggagcgattt aggtattctc ggctttttat
     1921 agaaatagtc aaggttcgat gcggaaataa gtctgtacag atttttcaga ccgaccttgt
     1981 tcttcacaag aattatctga tgatatgtag gaagcttctt tatatccaca cttccgaacg
     2041 ccgtattgaa atcgcctata tattcaagtt ttgcggtctt tctggtgtca tttactatgt
     2101 gcatatatat catacacagc atttctgcgt ccgcaaccgc tctgtgatgg tcaaaatcgc
     2161 cgagcttgaa atactttgct atagtatcaa gcttatagtt cttgataccc ttgagcgccg
     2221 ccttcgccac actcaacgta tcgactacag gattatcaaa gttctttccc attcttgcgg
     2281 cggcgcttct gataaacgaa acgtcaaact tcgcgttatg tgcgcagaca ggcgcattac
     2341 cgcagaatgt cataaagtcg ctgaccgcaa tatcctctgt cggagcgtcc gctaccatat
     2401 cgtccgtgat acccgtaagc tccgtgatat ttgacggtat cggcatcatc gggtttacaa
     2461 aagtagaaaa acggtcaact atctccatat tgcgcagttt taccgcacct atttcggtta
     2521 tacgctcgtt tgcagggtga agtcccgttg tttctatatc aaatacgatt atatcgtcct
     2581 cgatttttat cttatcacag ccgtctacaa ctgcgttacc gtcattgaca aagtacgctt
     2641 caagtccgta tattaccttg aagtcgggat cttccttgtt tatcttttca acggtattca
     2701 tcgcctcggg gtatgcctgg gcattaccgt ggtcggttat ggctactgcc ttatgccccc
     2761 atgcatgagc ctgctttaca agcacagacg gagctgtaac tgcgtccata tccgacatat
     2821 ttgtatgcat atgaagctct actctttttt cgggagcatt atcctgcttc ggttttacct
     2881 cgacaagcat taccgactgc ggattaaagc agtt
//
LOCUS       DAWAOG010000243         2904 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4426_length_2904_cov_2.211653, whole genome shotgun sequence.
ACCESSION   DAWAOG010000243 DAWAOG010000000
VERSION     DAWAOG010000243.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4426_length_2904_cov_2.211653"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          789 a          560 c          744 g          811 t
ORIGIN      
        1 gtttccggca agttgaacaa ttctattgta tatatagcag tttcgtagaa cgaacggtga
       61 ctgcactgcc tgccgtaatc atcgggcgga gtgatgctgc gtttgcgtaa aaattcctca
      121 tcgacatatt gccagacata cattgagtcg agcagagtac cgtcaaggtc gaacagcacg
      181 gcttttatat cgttcatatt cttctccttt tttggcttag atatattgct attgtatcac
      241 aataaggcgg tacggtcaat aagattttgc tgtcggcaag cttgcaaaat gcaagaaatt
      301 atgttatact tatttaagaa taaagaaaca ataagaacaa aggaaagaaa agaaaattgg
      361 aacagacaaa agaaaataag atgggcgtaa tgcctgtgaa taagttactt gttacaatgt
      421 cgttgccgat agttatatcg atgcttattc aggctatgta taaccttgtt gacagcgtat
      481 ttgtggcaca gataaacccc gatgcgctga ctgctgtggg catggcgttc ccgatgcaga
      541 gcctgatgat cgcattccag acgggacttg gtgtcggcat gaatgcactt gtatcacgct
      601 atttagggca gaaaaagcca tatgaagcag ggcttgcggc tacacattcg ctgatactta
      661 cggcactgaa ttacgtggta ttccttatag tgggacttac ggcactgcct gcgttcttct
      721 cgatacagac acactctgag gtcatcgcaa attatggtgt tgaatacctt tcgatagtat
      781 gctgtggctc gttgggactg tttttccaga tatacggcga acgtatacta caggcgaccg
      841 gaaagacgtt actttcgatg gtgtcgcaga tactcggtgc ggttatcaat atagtgcttg
      901 acccgatatt catattcggc gttgactttc ttgggatacc tgcaatgggc gttaccggcg
      961 cggcgatcgc cactgtaaca ggtcagataa tagcggcggc ggtaggattt ttactgcatc
     1021 atttccataa caaggaatta aggcttgtgc tcagaaggtt cattctgagc ttcgggacga
     1081 taaagacgat atatgccgtt ggaataccct ctattataat gagtgcgctg gtatcggtac
     1141 tgacattcgg tatgaatata atactgaaag catatgagcc tgcggcggct acggtgttcg
     1201 gagtatattt caagctgcag agcttcgtat atatgcccgt atttggtatg aacaacggta
     1261 tagttcctat catagcctat aactacgggg caggaaagcc caaacgtatc accgatactt
     1321 tgaagctggg agttatatgc tcggttgcga taatgataat aggcgtgttg cttttccagc
     1381 tgataccggt acagctcctg ggtctgtttg cacctaccga tgatatgata aggctcggag
     1441 aaacggcgct gaggatattc agtatatcgt tcccgcttgc aggtgtttcg atagtactga
     1501 gctcaggctt tcaggcactc ggaaacggtt tctatagtat gatggtattt atagtaaggc
     1561 ttattgtgcc gacactgcct tttgcgtggc tgttcggcat tatcggaggg gttgactgta
     1621 tatggtggtc gctcccggta agcgatctgt taggacttat tgttgcgata ctccttatgc
     1681 gtaggatcta taagaaagtc atagcaaaga tgtatgacag tagtgaagga tcttcggcac
     1741 aggtacagca ggcataagaa taaagcacaa acggaggaac tgattatggg aagaataatc
     1801 acaatttcaa gacagtacgg aagcggagga agtgagatag gcagaaaggt cgcaaaagcg
     1861 ctttcaattc cgtattatga caagacgata atcgatgtga ctgcaaagga aagcggtttt
     1921 gacccgaaat acatagagcg tgcggagcag acgactacca caagctttct ctataatctt
     1981 gcgataaacg gtatgtatac acagccgctg accgatcagc ttttctcggc tgagtgcagg
     2041 gtcataaagg cgcttgccga taagggtccg tgcgttatag tcggtcgctg tgcggactat
     2101 gtgctgaggg agcagtacag cacatttgat gtttttgtac attctacgga cgacttcaga
     2161 tgtgagcgta tctgtgaaca tgataatata agcaaggaag aagcgctgaa tgttatccga
     2221 cagaaggata aggcaaggcg cagacactac aagtattaca ccgagcgtaa ctggggtgaa
     2281 tgccttaact acgatctgtg cgtcaatact gcgacctgcg gtattgatga ggcggctgaa
     2341 ataatcgcta agcttgcagg caaataaaga cgtcgcattc agcggctgac gcctttaaga
     2401 atagtaaaaa atcggctgtc aacggggact ttaaaatcac gttgacagct ttttttgaaa
     2461 acagttaaac agagaaaata tttgaacaga aggaacggtg aacgatggaa tttgtaatgc
     2521 tctttttttc ggtgataatc attgtctgca tcgtcggcaa taagctgtcg acgaagatag
     2581 gcgttccgag tctgctgata ttcctggcac tcggtatgat atgcggttct gacggattgc
     2641 tgaagatacc gtttgataat tatcagctaa gtgaacaggt gtgtacggta tgccttatat
     2701 ttataatgtt ctgcggcggt ttttctgcca actggaaggc ggcaaagcct gttgctttgc
     2761 gtgcgggact tttatcttca ctcggtacga tagttacggc gcttattact acggcaggct
     2821 gtcattttct gataggattt gatataaaag agagctttct tatcggagcg gttatttcat
     2881 ctaccgatgc ggcatcggtg ttct
//
LOCUS       DAWAOG010000244         2903 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4432_length_2903_cov_2.170295, whole genome shotgun sequence.
ACCESSION   DAWAOG010000244 DAWAOG010000000
VERSION     DAWAOG010000244.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4432_length_2903_cov_2.170295"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    315..324
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2217..2226
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          832 a          630 c          490 g          931 t
ORIGIN      
        1 tatgagttga aaataccttc atatgtatta accaacgtac catccatatc gaaaattaca
       61 gctttgtatt tcttcataat gatttttatt cctcgcaata ttttgatgga gtgataaata
      121 ctatattcat ctttcgatta agctctttta tctgattttg tatatcggcg tttttcgtgt
      181 caattaaatc atcgtttaca cttattccgc cctctatcat agagatataa tttttattcg
      241 tgtatttatc atacccgtca aaacctgcaa gataaatatt tctgacgccc atattaaaga
      301 acaatctcat cgccnnnnnn nnnntttatt tgttccgtat aatcactcag agatttgccg
      361 ggagctatga gcataacgct acgatcagct atccggtttt gaatttcacg gatactctgt
      421 ttgtcgtcaa tagctatacg tttaaaatcg ttataccttg tttctatcat cgaaggatta
      481 tatagatgcc gttcatgctt cggcaacgat gaaagaatgc cgtttatatc tttcatcgaa
      541 agggtctgct tgtttaaaag atattgtgcg taattaggat ggcacatatt taccgacgct
      601 atgaaatacg caaccgaata gccccatgga tgctctttat agatttcact tatgtatctg
      661 tcgtatatct ccataacaga tacaacatca tatctggcaa ctacattatc gttgatatac
      721 tgtgttataa gttcggtgca cagattgccc gcccctcttc ccataccgta cacggatgag
      781 tcaatgataa tatcacgctt tgtcgggaga gtaacaagag cctgagcgtt tgaaaaagca
      841 agttgcaggt tattgtgcga atgatagcct atttttacag agctgtcaag attattatcc
      901 gccatataaa acagatgcaa caaatcatcc tgatagagcg tacccattgt atcaacaata
      961 tagaatgcat atggattcat ctcattgact ttttcgacaa gctgcagaaa ctcatagtct
     1021 gtatatgaaa cagttcctac aggctgcatg aataccttgt agccttttgc cataacctca
     1081 tatccgaatt tgaacgcctc ttcgatctga ttattatgga aagtaactct tatgccctct
     1141 acaccgctgt gatcacacgc tgatatctca tctatagata tatcgccata tgctaccata
     1201 gctacataaa gtacattatc agacttgttt tctatgcatg catttacatt ttctactgaa
     1261 gcaaacaatg agcagtcgat atcattttta ccggatttca gaaatccgca ctccactata
     1321 tcgacacctg catttgaaag gcgtctgata attgatttta ttctatgatt gccaaatttg
     1381 aagttattta cataaccgcc atctctaagc gtacaatcta ataccgaaat actcatttta
     1441 agcgtttctc ctcaaattat tcagtcttga attttgaatt gaatattgcg tcggcaataa
     1501 caaagtcatc gtcgtcattg atatcacagg cttcgatctt atttacttct ataaggaacg
     1561 gattatcgga tatacgtctg cctttattga gtatcagatc cgatgtatac acataaagtc
     1621 cgcaggtttc tgtatatata tccggaagat cctgagtacg cggtatgctg tcgagagtgt
     1681 agttaagagg ctttccgtcc ttccataaaa actccttaag catagcaacc gaatgagctg
     1741 actcgtactt gccgctgaca acagcttcaa tgcctttttc tatactttgt gctgtcatga
     1801 acggagcggt tgcatgagtg agaacataaa catccgcttt taccagcttt gcaaaacttg
     1861 acaaaacttc gttaaacgat gttgtagaca agtcgtaata cggatctctc tttaagaaca
     1921 gtattccttc agggagataa tcttttatcc tctcatcact gcagtacaca tatatctcat
     1981 caatcccatg cgttttgctc aaggtcgaca aaatataaga aataagcggt tttccgttag
     2041 taaacggttt gatattcttc agcggaagtc tttcgctgtt aagcttgatc gggacaaatg
     2101 ctacagtttt cataaagttc cccttaaatc atatagaatt ttattaggca actgctcaaa
     2161 tctgcaatac cgggtattcc gacacagata tgtaaatata tcgatcgctc ttaagcnnnn
     2221 nnnnnncaaa tacaataatg ccataaaatg cagatttgac gcgttaattt gcaaatttta
     2281 ttgtaccata aacaaagact tatgtcaaga agcaacatta caggtgcgat ccggttatta
     2341 tagccaaacc atgcaaaata caaactgcct gtcacaagtt cttatcaatg gttatagtat
     2401 acctgaagcc atcccggtga ttcagattgt agaaaaagtc cgttatttta aaagcgctta
     2461 tcctgaatct ctaccccttt aagtacactc tgatggcttt tcttacaact tttgttgata
     2521 aactaaattt caagcttcaa gcagcaacag ttttcatcaa agcgcatcag atcatcttgt
     2581 cttctccatt atcatctcta ccacctgctc ggctgtcgta cccacacagt caatggtaat
     2641 atctgcataa cggttgtagt aagcctcacg ctctgcgatt atctctgcta ccgtttcacc
     2701 cggacggcag gcgatgccgc gcgtttttat gttcgtaagt cggcggataa gctcctgctc
     2761 atccaccctg agatatacgc agataccgtt ttctctcaga tgctccatac ctgcggtcga
     2821 aaataccgca gagccgcctg ttgcaattat cgctttttta tcgtttattg acagcagtgc
     2881 ctgttcctcc gcctgcttaa atc
//
LOCUS       DAWAOG010000245         2894 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4451_length_2894_cov_2.007749, whole genome shotgun sequence.
ACCESSION   DAWAOG010000245 DAWAOG010000000
VERSION     DAWAOG010000245.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4451_length_2894_cov_2.007749"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1059..1068
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2321..2330
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          860 a          574 c          789 g          651 t
ORIGIN      
        1 atcaatatct gatgataact tacgaataca gattaccgca cgatataaat tcgttcagac
       61 gaagcgtagg ctggtatgag ctttcgccaa gacagcttga atgtgcgctt tcacgttcgg
      121 tgttcgcgat aacggcgctt gacggcgaaa aagaagtcgg ctcggcgcga gttgtcggtg
      181 acggcggcta tcagttcttt atcagcgacg ttattgttcg ccccgaatat cagggacagg
      241 gcataggcag aggaatgctt acgcggctta tggacaaggc gttgtcggta gccgatgagg
      301 gtgaaacgat aatgataaac cttatgtccg caaaggataa agagccgttt tacgaaaagc
      361 tcggatttat gcgcagaccg aacgatgaac gcggttgcgg aatgacaaga tttcttaaaa
      421 aataagaggt gcttatatga gcgtaaaggt atatattgac ggtcaggaag gaacgaccgg
      481 acttaagatc cttgaacgtt tcaaggacag gaacgacatt gaaataatga agatagatga
      541 ggacaagaga aaggacaacg atgagcgtaa gaagtttata tgctcgtccg acttcacgtt
      601 cctttgcctg cccgatgccg ccgcaagaga ggcagtagag cttgcgaagg gcagtaatgt
      661 ccgtattata gatgcgtcta cggcgcaccg tacaaatccc gactgggcat acggctttcc
      721 tgagctttca aaggagcaca gggaaaagat agcggcttca tcaagagtgg ctgttcccgg
      781 ctgctatgca agcggtttta tttcgcttgt atatccgctt gtaaaggcgg gcataatgcc
      841 gagcgattat ccggtggtcg ctcatgcggt atcaggctac agcggagcgg gaaagaaggc
      901 tatcgcagtg tacgaaagcg atgaaaagcc tgaagaattt tacagcccaa gacagtacgc
      961 actccctcag gagcataagc acttacctga aatgatgaag ataagcggac ttgactacaa
     1021 gcctgtgttc aatcctctga tatgcgatta tttcagcgnn nnnnnnnngc ggtatggtcg
     1081 taactgttcc ggtactgaca agacttctgc cgaagaaata tacacttgcc gatatccaca
     1141 aggctttcac ggagcattat gcagacgcaa atcttgtaaa ggtaatgcct gttatgggcg
     1201 agggcgttct cgaaaacggc tttctagccg caaacacatt atccggcaag aataatatgg
     1261 agatattcgt ttgcggaaac gatgaacgta tagtactttg ttcaagactt gacaacttag
     1321 gcaagggtgc aagcggtgcg gctgtacagt gccttaacat aatgatgggc attgacgaaa
     1381 cgaccggtct tgtataataa gtgaggtaat aacaatggtt gaatttgata gctataaatt
     1441 agtcgataag ggcgtatgcg ccgcaaaggg cttcaaggca ggcggcataa atgtcggcat
     1501 aaagcccggt tcgaaaaagc gtgaccttgc agtgataagc tgtgatacaa agtgcacggc
     1561 ggcggcaatt tacacacaga acaaggtcaa gggcgcgccg ataatcgtta caaaggaaca
     1621 ccttaagaac ggtattgctc aggcggtcat tgtaaacagc ggtaacgcaa acactattga
     1681 gatcgcagag cagatgtgcg agctttgtgc aaacgagctc ggtattgata agaatgatat
     1741 gatagtaggc tctacaggcg ttatcggaca gaagctgtcg atagaccctg taaagaacaa
     1801 tatcaagacg cttgtagaca gcataagcga ggacggaagc tcgcttgcgt gcgaggcgat
     1861 aatgacgacc gatacacgca aaaaggagct tgcggtagag tttacgcttg acggtaagac
     1921 ctgtcatatc ggtggtatca gcaagggcag cggtatgata aatcccaata tggctacaat
     1981 gcttgctttt atcacgaccg atgtaaagat aaatgcggag cttctgcaca aggcgctgaa
     2041 aagaacagct gatatcacct acaatatggt gagcgtggac ggtgatacat ctacaaatga
     2101 tacgctgtgc attatggcat cgggtcttgc gggcaacaag gagatcacag ccgaggacaa
     2161 ggattatgag gtgttcatca acggtcttta cgctgtaatg atgaatcttg caagagaaac
     2221 cgcccgtgac ggcgagggtg caactaagct gatggaatgc gttgttgacc acgcacctac
     2281 agaagctgtt gcaaagagcg ttgcactgtc tgttataaac nnnnnnnnnn tgccgagggt
     2341 gactttgata tttcaaaggt cgaggtcgtt ctgtcatcgt caaagggcag tgtaaaggtt
     2401 tgtgagaacg gcgcaggcgt tgacttctca gaggacgaag cgttcaagat actgtccgaa
     2461 gatgaaattg ttatcagaat agatatgcac gatggtgcgg ataagtcggt agcatgggga
     2521 tgcgacctga cttatgaata tgtaaagata aacgcagaat acagaagctg acaccgaaag
     2581 gacaaaagaa aatggaactt gaaaacgaaa agagagccgg catattaagc gatgctcttc
     2641 cttatataca gcagtattca aacaaggttg tagtggtaaa gtacggcggt aacgcgatga
     2701 caaacgacaa gctgaagcag gcggtcatgt cagaccttgt tctgctgtcg ctgtgcggta
     2761 tcaagatagt gcttgtacac ggcggcggac ccgaaataaa cgcaatgctg aaaaaggtcg
     2821 gcaaggagag caagtttgta aacggactgc gctataccga tgaagaaacg atcaacatcg
     2881 ttcagatggt gctc
//
LOCUS       DAWAOG010000246         2881 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4470_length_2881_cov_2.016985, whole genome shotgun sequence.
ACCESSION   DAWAOG010000246 DAWAOG010000000
VERSION     DAWAOG010000246.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4470_length_2881_cov_2.016985"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    777..786
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2639..2648
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          746 a          722 c          581 g          812 t
ORIGIN      
        1 tattctctta ggagaggtga aatcaaacgg cgtacccttt acacttgcta tctcgcctgt
       61 aggtataagt acagggctta cgggagtgta cttatcggca aatatctgtg caatatgatc
      121 cttgatatct cctgcatctg tgccgttgag attaaaatat gagtggttcg tgaaattcat
      181 tacggtatcc ttatcactca ccgcattata gcatatctca actgaatcag tcgttaaagt
      241 atactttaca tcaagcgtca aagtgcccgg gaagccctct tctccgtcaa cgctagtgta
      301 tgagaacaca acataaggag cgccgtcatc gccgtaatcc ttaacttccc atactttctt
      361 attgaagccg atattaccgc cgtggagcgt gttgccgttg tcatttttcg caagggtata
      421 ctccttgccg ttaagtgtga actttgcacc gccgatacgg tttgcgtatc tgccaaccag
      481 cgcgccctga ctgcttgtgc cggcaacata accgtcaagt gtatcatagc caagcaaaac
      541 atcgcccttg tcgccgtcct tatcgggtac tatcagtttt acaagcgagc ctgcatagtt
      601 tgtgatctcg gctttcatat tgccgttttc aagcgtgaat aagtagcagt ccttgccgtt
      661 gtgactgccc cagaatttct tttcaatgct catattattt accgtgaaaa ttatatttgc
      721 gttatcagcc gctgttttta ttttatcaca tcaagtgata aatgtaaacc catttgnnnn
      781 nnnnnnaata cgaatgattt atttcttttt ggcaaaagcc tcttttatca ctcttaccgt
      841 atgtatcaga aacgtgggcg caaagcccag gagtaacgtt attccgaaat ttgagatatt
      901 cataatctta gttccgaaca gatccgaaac tccgggaacg aatactgcaa ggcacaaaag
      961 gactattccc gcaaagaacg caagcagtga gaacttattg gcagtaattc caaggctgaa
     1021 aagcgactta tccccccgac aattaaaacc gtggaaaagc cttgcaatac acagtgtcgt
     1081 aaatgccatc gcacacgcaa gacccgaaga aacagccgaa ccgataaaat acgccgacat
     1141 aacagcgagc gctataagca cgccgccggc aactatgcgt acagacatag cctttgtgag
     1201 aaatgaggat ttactgtcac gcggcttttg cgaaagcagt gagcggtcgg ctctctcggt
     1261 gcttatggca atagcgggaa gcgagtcggt aaggagattt ataaacagga gctgaacagc
     1321 agtaaacggt gcgggaagtc ccggtatagc cgtacataat acagcgatta tcgccgcaag
     1381 attgccggac agcaaaaatt taacggcatt cttgatattt gcatagattg ctctgccatt
     1441 agctaccgat ttgataatcg tagcaaaatt gtcatcggtc aatatcattg aagcggcatc
     1501 tttcgatacc tgtgttcctg ttatacccat agcaacacct acatctgctt ttttcagtgc
     1561 aggagcatca ttaacaccgt cgcctgtcat cgcaactatt ttgcctttac tctgccaacg
     1621 gtctactatt cttatcttgt tatccggtga tacccgggcg taaacagaaa ctttttcgat
     1681 cataccatcc agctcctcat cggtcattgc atcaagctga accccgtcaa gacttatgtc
     1741 gccgtcacgc attataccga tttcccgtgc tatcgcactt gctgtcactt tgtggtcacc
     1801 ggttatcatt acgggtttta ttccggctct tatgcaatcc gcaaccgccg ccttgctctc
     1861 gggacgcggc ggatctgtca tcgcaacaag tcctataaaa atataattat cctcagtgtc
     1921 ggcggaactg ttttcagtga ctttataagc aaaaccaagc actctcattc cctgtccgga
     1981 aaaatgctca ttagcacaag caatatcctt tttatcttta tcggttatgc ctctgaccgt
     2041 accgttatcg cacaccgaaa ccaaacgggg caacaaattg tcggtagcgc ctttagtgaa
     2101 cattgtacgc ttgccgtcga tcatatggca ggtgctcatc agctttcttt cactgtcaaa
     2161 cggtatttct gatactctcg gataatccga acggatcttt tcgtaagccg taacaccgat
     2221 cagatccgac aatgcagttt cggtagggtc gcctatggcg tttccgtctg aatatgcggc
     2281 atcattacat aatgccatcg ctttttcaag aacatcaaaa cccgcattac aagccgaagc
     2341 gtcagcgttt tcttccctac cgttaagata cacttgcctt accgtcattt tattctgagt
     2401 gagagtacct gttttatcac tgcatataac cgatacacaa cccagtccct cgacagcctt
     2461 cagatctttt attactgcgt tttgctctgc catcttcctt gtgccgatag caagcgctat
     2521 tgtgactatg gaactcaacg cctccggtat tgccgctacc gcaagcgcta cagcaaacat
     2581 caacgaatca agtatgggag tgcctctgac aatataaagt atcataacta taagactcnn
     2641 nnnnnnnngc ggtatgctgt cgccggtcag tatctgcgtc attttttcta tgccattact
     2701 ttcacccgta agggagcttt cgttcacttg caatgatgcc gccgagatca ttcttccgtc
     2761 tgcgggaact atatctccct gctcaataat aacgatatca cctactgtta tgtcagccgc
     2821 atctatgatg cagacattac cgtcacgcat gaccttagcg gtgggtgatg acatagattt
     2881 c
//
LOCUS       DAWAOG010000247         2880 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4472_length_2880_cov_1.959646, whole genome shotgun sequence.
ACCESSION   DAWAOG010000247 DAWAOG010000000
VERSION     DAWAOG010000247.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4472_length_2880_cov_1.959646"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1442..1451
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          944 a          527 c          641 g          758 t
ORIGIN      
        1 cttgagggtc ttgaagaatt tttggagaaa gaacaggaaa tataaatggc taacaagaaa
       61 tgtcccgttg cgaaaaaatg cagtggatgt caactgtcca acatgacata cgaacagcag
      121 cttgaatgga agcagaagga taccgaaaag ctgctcggca gtttcggcaa ggtatcgccg
      181 ataatcggcg ctgacgatcc gtataactac agaaacaagg tgcaggcggt attccgctcc
      241 gacagaaacg gcaggataat ttcgggtgta tatcagtcgt ccagaaacgg catagtcgga
      301 attgacagct gtatgcttga cgataaacgt gcggacgaga ttatagtagg aataagagga
      361 ttacttaaat cgttcaagct acaaccgtat gacgagggta cggagcgtgg ctttttacgt
      421 catgtgcttg tccgtgtcgg aaagaatacg ggagagatac tggttactct tgttggcgga
      481 aactcaatgt tccctaaaaa gcacgacttt gtgaaagcgc ttgtaaagca gttccccgac
      541 atcacaaccg taacattctc ggtaaaccgc actcccgaaa tgcttactct cggaaataat
      601 aacgacgtgc tttacggcga gggatacatt acggatattc tttgcgggaa aaggttcaga
      661 atatctccgc actcgttcta tcagataaat cacgcacaga ccgaaaagct gtacgactac
      721 gctataaagg cggcaaagct cacgaagaag gacgttctgc ttgacgcata cagcggaata
      781 ggcacgatag gcataatcgc atctgactat gtaaagcagg tacagggcat cgaatacaac
      841 gcatcagccg tccgtgacgc agtaaagaac tgccacgaaa acgggcttac ccgcaacatt
      901 gctttcaacc ggggcgatgc aggcgagttt cttgaaaaga aggcaaagct aggcacgcat
      961 tatgacgcag tgatcctcga cccggcaaga gcgggagcgg acaggaagtt tttaaactcg
     1021 ctcgtgaaaa tcgctccgga gaggatagtt tatatttcgt gcaacccggc tacgcaggca
     1081 agggatctga aaacgctcac gaaaaagtac actgtgaccg atattcagcc gtttgatatg
     1141 tttccgcata cgaggcatgt ggagtgcgtg gtttcgatgt caaggaaatc agagtaaatc
     1201 gggataggtg gcttaacggt gcggtttgtg cgtgttgggc agactgtact gttttggtag
     1261 aatatggtaa tttagcgtat agggtaatat gtctgcggtg acagcagttt cgtagcgata
     1321 cacggtaagt gggtcgggtg tgcggaagtg gtgtgatagt gggaaggaaa gatgtaaatg
     1381 taatttataa taaaatcaag cttgataaaa atacagcatc tcgattttaa tggattttca
     1441 annnnnnnnn naaaacgtaa aggagcaatt attgtgaaaa aagaatttct tacatccaat
     1501 gccaagtttg tctattccaa aaacgaatta ggctatcaag aggtattaga tagatttaaa
     1561 gatgccaaac aaattacaat tataactttt aatatttcag agaaacagaa cacattggtt
     1621 aatgccctaa aaaaagcagg agatagctgt atcatcaatg ttattacgaa cataccaaat
     1681 agatgggaaa cctattacgg tgacgctttt cgtgataaag cacgaaaaaa aataaatcta
     1741 tacatgacaa aattaatgcc tgaaagtctg ggggcgaaga cagctgtttt tttgattttt
     1801 caaatcacgg aaaaattatt atgacagact ccattgtcta cgttggttct gctaattatt
     1861 cagaagaaag tgcaaataat acagagtttg gtttcttgtc agaagataag gagttgatag
     1921 aatttattaa tatggacgtt ttaccagata ttcaatcatt agctattcct tattatgaat
     1981 atgattatac agctgttttg cttgaagcaa atgttgctct tgcagctgtt tacaatatta
     2041 aaaatgagtt gtttgaagaa gtgtataggt tacacgatga catagatggc gaatggtatt
     2101 actatgaata caacgaggcc agtttaacgg tttttacttt agataaaacc ctacaaatca
     2161 tcgatgaagc ttgtaatgtt gcaagagatg tctatgatgc tattgatgtt atcaccaatg
     2221 gcgatgagga tgaaactact aatgccaatg atgaatatga tgaaatatta gcagtagcat
     2281 cacatattga agaaattaga tcctacgata cacttataga attatctgaa tttgattctg
     2341 gagaatttgt aaatcagcaa cttcaaaaag aatatgcaat ggaagcatat gaggaaaatc
     2401 ttgaaaattg tatcaatagt gcttcagaag atgctcaaaa tatcgtgtta gacttaaccc
     2461 aaacggcaaa agacgatatt gacaaattat tatctgaact gtgataagta cgctaatttc
     2521 attgataatt tgcgtgcaag agaaataaag aaaatcagcc caaaaattga taatacataa
     2581 catcacaatc gagaggggtt cgtacatatt caaaaattat aattcaatct caaccgtcac
     2641 ccccgacttc agctccacag aaaacctatc atcacacacg acaatctcct taatccaccg
     2701 ccgcgcaagg ctctcgtcaa actcggtaag ctcggaatcc tgctgcttga tgaactcgtt
     2761 cagctcattg atacacttga cctgctcgtc acggacggat gtgtcgatga agttcttctg
     2821 cttcatctca cgaaattagt gtattcatat gtgaggtttt cctaccctaa acaatattcc
//
LOCUS       DAWAOG010000248         2872 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4483_length_2872_cov_2.320554, whole genome shotgun sequence.
ACCESSION   DAWAOG010000248 DAWAOG010000000
VERSION     DAWAOG010000248.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4483_length_2872_cov_2.320554"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1155..1164
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          739 a          726 c          620 g          777 t
ORIGIN      
        1 accttgcaag atgcgatatg tccaaggcaa tctcggacgc atttgcttac ggcaagcttg
       61 tgcttgcaac gcctacctac aatgccgatg tgttcccgtt tatgagaaca ttcattgagg
      121 gtcttaccga aagaaactat cagaaaagaa cggttgcgtt tatcgaaaac ggcgcatggg
      181 cacctatggc ggctaaggta atggcaggta tgtttgaaaa gtcgaaggaa cttaatatcc
      241 ttgacaagac ggtcaagatc acctgtgcgc tgaatgacgc aagcgcggca caggtcgatg
      301 agctggctga tgagctggca taagtaaatc gaatactaaa aacgctcccg actgatacag
      361 tcgggagcaa ttttatattc tgttttattc tcttactctc tgtttttcag cacctcggca
      421 ggtgtgatcc tgttcagctt tcttactaga agagtgtttg tgagaagata aaccagcata
      481 accgcaataa acaatgcggg atagaaatac cagccgaatt caaggtttat tccgcacgca
      541 atgttcgcca caaatgcagg gaatattgta tttataacga gctttgaaag cggcaggcag
      601 acaacagccg atatcgctat tgtgatcgcg ttaccgtcaa ggtacagcct ctttacgtcc
      661 tttctgctgt agccgaaggt cttgagcagt gaaataccaa acgagcttct gtctatcatg
      721 acgctcatca taaggtacat taccactacg aaaattacag tagccgcacc gataagcgtt
      781 acgatcattc cggtcataag ctcggaaaat acctttgacg agcgctctac atcagccttt
      841 gtcgttaccg aatagatcct gccctcgtca atgtcgagcg gtttatccga cagaaccata
      901 ttgtaataat catcgtcctg cttgaaaagc tctctcatgc tgtcgatatc cataaatacg
      961 gcaagtcctg aggaataatc gcttatgcag tcgacagtga aagcatagtt catatcagcc
     1021 gcttcatcag taagaatgaa cgtatcaccc tcgacaactc catatctctg tgctacggaa
     1081 agtgacgctg tgacaatgct cttgcccttt ttcacatcaa cgttattata ataaggatta
     1141 tcgctgtcta tgccnnnnnn nnnntcaaga atatatccgt ccttttcctt tgcaagcgat
     1201 tctacatagc acgcttcacc gccctcgggc acttcggtcg tggggtattt cagcgtatac
     1261 atatactcgt atgtcgtgcc tgcaagcgtg tctgtagtga catttgcaca catcgaataa
     1321 cagtcaagtc cgagcataag gataagcatt gaaacaaaca tacaagccgc aaccgtaatg
     1381 ttcgctctca tctcacgcag gaactgacgt attctgaact ttcttacaaa tccgccgctc
     1441 tttatctcca tgcggcttat gttcttaacg ttctgctcgt ttctcataag cgacagagca
     1501 ggtgcggaaa gtcttttatt gataacaagc gcatttacta ttgccgctat tacgggagga
     1561 attacaacgg cgtatattac aagataaagc ggtatcaccg ttgcaaaatc aggcagtgag
     1621 aaatatgcgt atgtatcctc aagctgtgac tgtatcgaca tgggtgaaaa gcctatcgcc
     1681 gagccgataa cgccgccgat aaacgctatt atcgtgggga gcgttatgta gtgccgtata
     1741 agtgcaccct tagtcacacc gagagcgtac agcgcgccta ttacactgct ctccctgttt
     1801 atctgatgaa caacgaatac agagatcaca taggcaaaca gtgccataag tattacgcaa
     1861 gccgcaagtc cgccgtattt gttcgttaca acatcgcctg ccgcagctgc gatacgaacg
     1921 ttatccgctc ttttaacaaa cgatgtgaga ttggtcagat caaggtcgaa tgcagcgtca
     1981 agcagctttc cgatattgct cttaagctcg tgtattccgt ctgcaagctc acctgctccg
     2041 tcctttgcgt ctttcgcgcc gtcagcaagc tcctttgcgc cgtcttttgc gtccttagcg
     2101 ccgtttgcaa gctcctttgc gccgtttacc gcatcgtttg cacctgttga cagctgctct
     2161 gctccgcggg aagcactgct aatgcccgat acaagttcag atgagccgct cttagcactt
     2221 ttaagtcctg ttgcaaggct gtccgcaccg cttacaagag cgttcattgt cggagggata
     2281 agtcctgcgg aatttgctcc gactatcgct ccgttaagag atgaagcacc gcttgacaac
     2341 tcgctcagcc ccttatcaag agccgatgcg ccgtcatata ttgcagtcat accgctgtgg
     2401 agctgtaccg tgccgtccga taagctctta agtccgtcat acagcttatc cgcaccgtcc
     2461 gataaatcgt tcataccgtc atacagcgta tctgcaccgt ccgacagatc tttaagtccg
     2521 tcgtgcagct tgtccgcccc atcggctaaa tcgtttatgc cgttttcgat ttcggtacgc
     2581 ttttcaagcg cctcgtttac cgtttcaaga taatacttgt cagtaacttt cgtatagtca
     2641 aaatcaaaat cctctataag ctctttcagc ttatcatcgg tcatatcatt tccgagaaga
     2701 tagccgtatg tgaaagtctg cgccgatttt gagctgtccg ataccctttc gtactcgctg
     2761 tcggtaacaa atatcaagcc gaaattcctg cttgatacag ccatatcgga catattctga
     2821 tacggtgcgt catagtcggg agaagcacct ataccgcata cgatatactc cg
//
LOCUS       DAWAOG010000249         2866 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4491_length_2866_cov_2.204909, whole genome shotgun sequence.
ACCESSION   DAWAOG010000249 DAWAOG010000000
VERSION     DAWAOG010000249.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4491_length_2866_cov_2.204909"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          827 a          649 c          622 g          768 t
ORIGIN      
        1 cgccaatact gactgggggc acctggggct tgcctggtct gcccgaggaa acaagtttcc
       61 tctaccacaa aatcttcgat ttcgtgcgat tccggcgtgc ttgctcttaa tgatagtgat
      121 ttgctgattt gcttagatat aaaaataaag gacactcgaa aatcgaatgt cctttatact
      181 ggtgcgggaa atgggacttg aacccacacg ccaatactga cacaagcacc tcaagcttgc
      241 ctgtctgccg attccagcat ccccgcaaat cagcataatc tattatagca gaataaaaac
      301 ggtttgtcaa gcgctttttg aaatttttct aaaaaaaatt caaaaaggca gcaaaccgtt
      361 ttagaattat ctgttgcgca ttttaagctc gcggtcgatc gtacgctttg cgtcccgctg
      421 tgccatagca tcgcgcttat catgaagctt tttacctttg caaagtccta cctgaacctt
      481 taccctgctt cctttaaaat aaacggaaat cgggataagc gtcagaccgt cctgctttac
      541 ctgtccgagc agtttcatta tctcgcgctt atgcataaga agctttcttg ttctcagcgg
      601 gtccttatta aagatgtttc ctttttcata gggtgatata tgcatttgct ttatatacgc
      661 ctcgccgtca tctatcgaga tccagctgtc tttcagatta agcccgcctt cccgtatcga
      721 ctttacttca gtgcctgtaa gctctatacc cgcttcatag ctttcaagta caaaataatc
      781 atgacgcgct tttctgttgt ctgctatagt tttgagtgcc gttttctcca ttcaaacccc
      841 tcttttctta taaataaggt ataaaccgtt ttattctcga ccggacagtg ctccggtatt
      901 cgctttcata aatgaggtat gaatattatt ttactataaa accgatattt ttgcaagcta
      961 attgcaaaaa attccgagat tttgcaataa cctaaatcaa aacttgcgta aatatgcggt
     1021 gaaacaggtc aaatttgtgc ttttttgagt tttttaaact tttttatgaa aatttttaaa
     1081 aaactattgc aaattttgcg tatatgtgat atactttttc ttgcagttaa aaaacactta
     1141 cggctttggt cgcaaagaat atactaaaac ttaccgatag agtcggtttt ctcacaaatt
     1201 aatttctgaa aaaagcagaa aaaccgagca tatccgtaaa attgcggtat ttcgtacttg
     1261 agccgaaaat atcaggagga tttaacaatg gcgttaaaaa atcagtatct tatcgacctg
     1321 ctcgaaacag ttaagaaaag aaatgcaggc gagcccgagt tcattcaggc agtaacagaa
     1381 gttttcgaga gccttcagcc cgtagttgaa aagagacagg atctcgttga cgcaggcgta
     1441 ttcgaaagaa tagtagagcc tgaaagacag atcatgttcc gtgtaccctg ggtagacgac
     1501 aacggcaaga ctcaggtgaa cagaggcttc agaattcagt tcaactcagc tatcggacct
     1561 tacaagggcg gtatcagact tcacccctcc gtatatctcg gtatcatcaa gttcctcggc
     1621 tttgagcaga tcttcaagaa cagccttaca acactgccta tgggcggcgg caagggcggt
     1681 tcagactttg accccaaggg caagagcgac ggagagatca tgcgtttctg ccagagcttc
     1741 atgacagagc tttacagaca catcggtcct gactgcgacg ttcccgcagg cgacatcggt
     1801 acaggcggac gcgagatcgg ttatatgttc ggtcagtaca agagactgag aaacgaattc
     1861 tcaggcgttc tcacaggtaa gggcttaaca ttcggcggtt cacttgcaag aacagaagct
     1921 acaggctacg gtctttgcta cttcacaaac gaaatgctca aggctaacgg caagagcttc
     1981 gacggcaaga cagttgttat ctcaggttca ggtaacgttg ctatctacgc tacagaaaag
     2041 gctacacagt acggcgctaa ggtagttgca ctttctgact ctaacggcta catccacgat
     2101 cccgacggta tcgagcttga cgttgtaaag cagatcaagg aagttgagag aaagcgtatc
     2161 aaggaatacc tcaacagaac atcacacaag aacgttacat acacagaggg ttgctcaggt
     2221 atctggacaa tcaagtgcga tattgcactc ccctgcgcta cacagaacga aatcaacggt
     2281 gagtcagctc aggctctcgt aaacaacggt tgcttcgcag ttgcagaagg tgctaatatg
     2341 ccctctactc ccgatgctat cgaagtttac ctttcaaacg gtcttcttta cggtcctgca
     2401 aaggctgcaa atgccggcgg tgttgctaca tcaggtcttg aaatgagcca gaactcaatg
     2461 cgttacagct ggacatttga agaagttgac gctaagctcc acgacattat gaaggaaatc
     2521 ttcaagcagt gcgacacagc cgctaaggaa tacggcttag aaggcaacta catggcaggt
     2581 gctaacatag caggcttctt aaaggttgct gaagctatga aggctcaggg ttgtgtataa
     2641 tgatccgatt tcgctaaaac tactccctta aaggagggct ttatatgaaa cacggtactt
     2701 tatctcaggg caagaagagc acggtagatc ctaagacctc gcttgtaaga aacgacggaa
     2761 gcgttaagcg taccgatgac ctttcgagca cgggactgga atttcataag ccgtttactc
     2821 acggcaagcc cgataaggat aataagtaaa tagaacgacc tccgca
//
LOCUS       DAWAOG010000250         2865 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4493_length_2865_cov_2.189680, whole genome shotgun sequence.
ACCESSION   DAWAOG010000250 DAWAOG010000000
VERSION     DAWAOG010000250.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4493_length_2865_cov_2.189680"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          790 a          615 c          769 g          691 t
ORIGIN      
        1 agtataagca gctcgaaggt gacccgcaga taaagggtaa gataaagcgt aaacagcagg
       61 aaatggcaca gcaacgtatg atgcaggaag tgccgtctgc cgatgtggtc gtgcgtaacc
      121 ctactcacta tgcggtagcg ctgaaatacg acaggaaggt agcttacaaa gcgcctgttg
      181 ttgtggcaaa gggtactgac gcactggcgc tgaggatagt cgcggtggcg caggagcata
      241 atgtatatat tactgagaac cgcccgctcg caagaggact ttatgaggcg gtggacatcg
      301 gacaggaaat tccgagagag ttttacacgg cggtggctga ggtgctggcg atggtttatg
      361 aagagcaaca tcggcggctg tgagccgttg caaattccgc ctttggaaat gttgcgattt
      421 ggcgacggat ggcgaaacgg cggggtttaa cgattgtgct gatgttcctg gcgagagaca
      481 gtgcgagctt tgcaacgata ttgcagacag ctgggacagt tcaagcgatc cctcagtctt
      541 gacggcagaa aaagtaagtg ctatttgaag gtttgactta tctcattaag cggtaatttt
      601 catggttaaa aagatgatga cgaatgtttt tgcggtattc gttatttttg tcgtacttgc
      661 gatcatcatt cctctgccga ctccgatact ggattttctt ctgataataa atataggact
      721 atctcttgtc atactcctta tgacaatgta cataaaaaag gcgctcgaat tttcgatatt
      781 cccgactata cttcttctga caacggtttt ccgactgtcg ctgaacgtat ctaccacacg
      841 aggaatactt tcaaacggtt acgcgggcga ggtcgtaaag accttcggtg aattcgtaat
      901 gggcggtgac gctattgtcg gatttatcat attcgtaatc attgtaatag taaacttctt
      961 agttatcacc aagggttcgg aacgtgtgtc ggaggttgcc gcaagattta ccctggacgc
     1021 tatgcccggt aaacagatgg ctattgacgc tgacctgaat acaggtgcaa taaccgatga
     1081 ggaggcaaag atacgccgtg cggaggtaca gcgtgaaagc gacttcttcg gcgctatgga
     1141 cggtgctacc aagtttgtaa agggcgacgc gataatttcg ataattacgg cgcttataaa
     1201 ccttatcggc ggcgcggtgc tcggactgat gcacggacag gatataaaca ccgtgctttc
     1261 tacctactcg ctggcaactg tcggtgacgg tctttgctcg cagatacctg cacttatgat
     1321 atctgttgca acaggtatgg tcgttacccg tgcggcaagc acggacagtt tcaacgcgga
     1381 tatcaagcgt cagtttaccg cacagcctaa tgttatgatg atcgccggca ttgtcattgc
     1441 cgcccttatg gttatcccgg gcttcccgaa gctgatactt ctcggcgttg gcgcggcact
     1501 gtttatattc ggtctgagat tatcaaaatc caaggcgaag aaagaggcag ctcttgccgc
     1561 tcaggctgaa cgcgaaagca tggcaaaaat gcaggatcag ccgacctcgg acaacgacta
     1621 ttacagggat atcgacaacg tattcaagct tctgaatgta gagcagatag agatggagtt
     1681 cggctacagc ctgctccacc ttgttgacga aaagagcggc ggtcacttca ttgaccgtgt
     1741 ggtaatgttc cgtaagcagt ttgcgatgga tatgggtatg gtaataccgt ctgtaagaat
     1801 gacggacaac cccgagataa accccaacca gtatgttata aagataaagg gcgaagaggt
     1861 agcaagagga gagatccttt cagaccacta cttagcgctt gacaacggcg atgttgtaaa
     1921 ccctgttgac ggcatagaca ctgttgagcc tgcgttcggc attcccgcaa agtggataag
     1981 cgcagacaag aaggtaatgg cggacgttgc gggctatacg cttatagacc ccgtgtccgt
     2041 aatgataact catctgtcag aggtcataaa gcagcattgc agtgagcttt tgtcgcgtca
     2101 ggacgtaaag acgatggtag acaacataaa gcagaccaac cccacgctta tcgacgatct
     2161 tatacccagt actatttcgc tcggttatct cgaaaaggtg ctttgcctgc tgctgcgtga
     2221 gggcgtaccg ataagggata tggaaacgat actcgaaacg cttggcgacc acgcgggtgc
     2281 gcttaaggat atagatattg tgacagagta tgtaagacag gcgctcaagc gtaccataac
     2341 aagacgcttt gcagaggcga actcgctgag agttattacg gtcgacccga aggtggagga
     2401 tactattgtc gcaagcgtaa agaaatcgga cgcaggaagc tatcttgcga tggatcccga
     2461 tctgatacag aaaatcgtca acataacaag cggagagata gacaaggtaa aggacgtaat
     2521 accgaacgtt atcatactta cctctccgat agtaagaata tacttcaaga aactgataga
     2581 tcaattcata ccgaatataa cggtgctgtc ctacagcgag atagacaata cggcacagat
     2641 acaggctata ggcaatatag cgatgtagcg ccgcattgtc cggaatatca ggaaaggagc
     2701 gttctatggc ggtatcggta cagaacaagg agcggcttcc cgagctcctc gaaaagtaca
     2761 ggatcacagg cgacataaat gtcaggaacg agatagtgct gatgtatatg gatcttgtca
     2821 ggataattgc ggtatctatg cataatatct atgcgggata tgccg
//
LOCUS       DAWAOG010000251         2864 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4496_length_2864_cov_2.128159, whole genome shotgun sequence.
ACCESSION   DAWAOG010000251 DAWAOG010000000
VERSION     DAWAOG010000251.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4496_length_2864_cov_2.128159"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    370..379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          724 a          628 c          593 g          909 t
ORIGIN      
        1 ccctgttttc gactttattt ttaaatcttt attgaaatac gtctgacttt aaattataat
       61 tttattagca gaatttattg agaggtggac gtatatgtca tatgtacttg aaataactga
      121 cgacaacggc gagatcaaat atgtttcaac aggtacagcg acagtatatt gtcccgagta
      181 tgcttcaaga tatgaggaca aaaagagcac catgacggta ggaactacac ttataaaaaa
      241 cggacgtttt tcgtgctttg aggtaagaca gcttagttgt gagctttgcc ttaaagcgta
      301 caagagaaac cgtgcgaggt cgttttttct tacatatccc gataatttca aataataatg
      361 gtcgtggcgn nnnnnnnnng ttttttaaac agcttatcgg atcgtgaaac agccgctcaa
      421 caagtcgcgg acacgattct ttaaggttcg gattattata agttacagcc ccgatgaata
      481 tcggggctgc agctttataa caattcttta ctgtcgtttg cttcactttg gacgttgttt
      541 ttcagatatt tctcagcctc ctgcacgggc atcggtttcg catacaggta accctgcgcc
      601 gtatcacagc cgcagctttc aaggaattga gcctgatcgt ccgtttcgac accctctgca
      661 ataagtccca gaccaacgtc ttttgacatt gagatggtgt gcattattat cttcttgccg
      721 cgatcattgg acatatgcgt tgagaaaaat tcacggtcaa gcttcaatac gtcaaatctt
      781 gtatcctgga gtgtattcag cgaagaatat cccgagccga agtcgtccat gagaagggta
      841 aaaccgtgct cctttatctt ttctgtcata cgcagtgtgg catcattttc aatgctctcg
      901 gttatctcaa gctcaagcag gtgctttggc aatctgtact tttcaataag accgtcaaga
      961 acatcaagga agctttcatc gtcaaggttt gcgcgtgaaa cgttgacgga tatcggcagc
     1021 ggtgtgaagt gcctgtctat ccagtcacgc agtaccatgc aggcgcactc ccacacataa
     1081 gcgtccagct ttgtgataaa tccgttttct tcaaaaagcg gtataaatct atcgggatat
     1141 atcattccac gttcgggact ttgccacctt acaagcgctt catagctgat agcttcaccg
     1201 cttgatatac tgaacttagg ctgaagataa atgataaatt cgccgttttt gagtgctttt
     1261 tgcatatgcg actctacaaa cttgcgttct tttatcgctt tctgcattcc tttattgtaa
     1321 aacgctatgc tttcaagaac gtttttcttt atcgactgcc ttgccatagc cgcatagtcg
     1381 cacatggtcc ttatcgatat attgcggtca tctatctgat atacgccgaa tacaaattca
     1441 tagctgtatc ccctgaaccc cttaagactg agttgcagct cggacacagt attcaatata
     1501 tcatttttgt cggtatacgg tgtcaacagt ataaacacat caccgctcat tcttgcgccc
     1561 atctgctgtt tttcgcagaa gttattgagc tgacgtgata tgaactgaag catttcggtt
     1621 cccgcgtctt ctccgtagtt ttcgtttatc agcttgaagc gctttatatc aaactgtata
     1681 agcgcaaatt ttacgtcggg atatgctctg agtctttgca agatcatatc atggaacatt
     1741 ataggattat ctgtagtggt ataataatcc agaatttcga tgttcattac ctcgtcaaac
     1801 tcaagctctc tgatatgacc tgcgatttcc gacatcttgc cgtctttttc tgcaagctgt
     1861 acataaatac agtaccatac gggatttccc ttgtcgcaca gacggatatt cactgaaaac
     1921 gagctttcgg ttattttgtc atggaaagaa ttacttattt tgtggcagaa attatcgtaa
     1981 ataagcttat catcgggata aatcgctccg ctaagccata ttgagcgcaa aggtcctaca
     2041 gaagatgata taaggcactt tggcgtatgg tcgttctcca taacatacca tttgaagttt
     2101 aggtagtcca tatagaaata agtgcacttg tcgtacaatg agaatatctc atgcattctg
     2161 agctgataat tgttgagcgc ttcgtttgct tttccccgaa tatccaaaac cggtcgagca
     2221 atctgatcgc gttctgcttt tttcatcctt ttctgctcat acataagccg gtcggtgctt
     2281 cgcataagct cgtttatatc ttcaatgctg ctcacagtac cgtaagagcc gccgatgctt
     2341 atggatacat cgtagggctt tccggcagtg cggttgaact tgtcgatctc tgtctgtatt
     2401 tcaccgacaa gtcttgacag ttcacgctgt ttgtctttgt tatatggtac ggtaagtata
     2461 aattcatctc cgccgaaacg cgcatatatg ccgttgttcc cgctgcctgc ttttatggcg
     2521 tttgcgactg tttgtattgc tatgtcacct tcggagtgac cgtaggtgtc gttgatttct
     2581 ttaagccgat ccatatcaca ggatacaagg ataacgtcgc acttttcgtt ttccgccata
     2641 agctttccca tacgttcaaa atatccgcgt cggttataga tatttgtcag cggatctatc
     2701 gagtgagtca gtgacagatc gttgtttgcc tttctgagca gatattctat gcggaagttt
     2761 tcaagtgtct ggtttatcga ttcgaccagt cgctgaacat agtagaagct gcccggattt
     2821 accagaaggt cgtctgcaac tgcggcgtaa tagcctatga cctg
//
LOCUS       DAWAOG010000252         2858 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4511_length_2858_cov_2.064217, whole genome shotgun sequence.
ACCESSION   DAWAOG010000252 DAWAOG010000000
VERSION     DAWAOG010000252.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4511_length_2858_cov_2.064217"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1510..1519
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          757 a          714 c          542 g          835 t
ORIGIN      
        1 gcattgctct gcgctcttac aggcttgccg ctttgctgat agtcatgtgc cgtcggatcg
       61 tttccccata cagtccagtc gcgctttgcc gcttccttct catagaaatg agggtcttct
      121 ccgcgctcgt ttataaagtg ctccgctaac tgcaaccatt tcttgttacc ggttgtgcga
      181 tacattttca taagtgcaag ctctacctcg ggatgacccg gtacgccctc gtttttttca
      241 gtgataaaat gcttatagat atgctccgca ttcttttcca taacatcaag gagctttttc
      301 ttaccggtag cctcatagta agcgcaagcc gcctccatca tatgacccga gcagtaaagc
      361 tcgtgcccct ccagcaggtt cttccagcgc ttatctctgt cctttattgt aaaatatgta
      421 tcaagatagc cgtcctcatc ctgcgcggcg gctattttgt cgataagctc gtcaactgtt
      481 tccgtcagcg tttcatcggg ataaagcgca agcgaataag ctgccgcttc tatccacttg
      541 gcggcatcgc tgtcctgaaa taccattccg taaaagccgt caccaacatc ctctccgcgg
      601 agcgcctttg ccgcattctc aaagttcttt acaacgtggc ttttctccgt atcgggcgct
      661 ttgtcgcaca gtacgctgta ctgataagga attaccgtat ctcttatcaa tttctgatat
      721 tttccgataa aaccatctga cttatatccg gtgactttta tgtttttctg tgttatcatg
      781 ataattctcc gttctcaaag caaacgcttg acttactctc atatatgtat tataatctta
      841 tcgacaagct aatcaaatag ataaaacgca caaaaatatg gagaatattc atgaacgaga
      901 tcgttgttac aaatactgct cttcctacgc tctgcggatg cggtttattt gccgcttctg
      961 agagctttat ccacacagac agaataccgg atttttgcat actgatatat gtaattgacg
     1021 gctgcatcta tgtcacggaa gatgaaaccg actatgaagt aaagacaggc gaactgctga
     1081 tacttaaaaa cggtgtacat cacttcggta agaaaaagat acaaaaaggt acaaagtgga
     1141 tattcataca cttcagaata acccgacaca gcggcggaac gccgttttat cccgacgctt
     1201 ccccgcttgg tgcttatgac gtgacaaccg aaagccgtct tactcttccg aaatatatga
     1261 atagcatttc aaaacggatc gaaaaggagc ttaactcatt tatagaatat gctcactcaa
     1321 acgacgtata taaaagatgg tttatcaatc acaggctttt ttcgctgcta tcttcacttg
     1381 caataagttc acacggcttt tcaaacgatc ttacactcag cgacaggatc tgtcgctatc
     1441 tttcggataa tcttgccgtg ccgttcagct ccgaaagcat cgaaaaagag ttttttctca
     1501 gttacaaatn nnnnnnnnnc aattcatttc tgtaatgccg aatgcgacag ccaacacggt
     1561 aaggatggaa gaggcttgca gattgctctg ctccactctt atgagcataa gcgaaaccgc
     1621 cgacaggctc ggcttttccg atgcattgta tttcagcaga tgtttccgca gtttcacagg
     1681 aaaaagtcca agcgagtaca gaaaagaaag cgcaagatat ttttaataac gcaaaagcct
     1741 gagaagaaaa attcctctca ggctttaatt atgcttatgt attcttgtaa ttactctccg
     1801 acattacagc tcattattcc gagtgcaaac ggcagatatg ccgctctgtg tatctctttt
     1861 tgcttaaagc cgttcttctc gtaccagtta cgcactctta cgctctgatg tatcgcgcct
     1921 gcgttaagca ccgtgccgcc cattgagaaa acctcatttt tagcatattc aaggcacttt
     1981 gctcccacac cgccgtatct cttgtcggga agcaccgaga acaggtgcag ttcatacacg
     2041 ctgtcgtcct tgttttcaat agcaaagcag gctatcagct cctcttcctc aaatgctccg
     2101 tagaaaatat agccccactc cgcagacagc tgtaaacggt caagcgtaat aaaagcggcg
     2161 tgctgaggac aattcatttc tgtaatgccg aatgcgacag cctccgtttc atagcttttg
     2221 cgaatgacat ctacacattg cgaaagctcc tcatttgtca gttttctgat agtcatactg
     2281 ttcacccttt caattttctg tatcagcatt ataaagaaca aatattaacc ttattttaac
     2341 agaagtcgct taaaaaaacg ttaactgctc gtttttttcg ggaaaatcgt aaagatacga
     2401 aaacacctcg gcgggatcac acataacgcc gtgcttttcg cattcattac agaatatgct
     2461 cataagcctg ccgttgttaa cactgttgca gatatactca tttccatact gcgatatata
     2521 ccgctgtttc attcccggaa aatgcctgtc aagcgcagag tagaaatgct ccctatcgcc
     2581 gtcccgcatc gtagttccta tccccataca gataatgccc ttcacccctg cgtcaaagca
     2641 gtatgacagg ataccacgca gattttcttc cgtatcattg ataaacggca gaacaggggt
     2701 aaaccatacg acagtgggta tgccgttttt ctgcattgct ttaagcacct cataccgccg
     2761 ttttgttgta catacattcg gctctaaaat ccggcataaa tcctcatcgt atgttgtcag
     2821 cgtcatctgt acaaccgtct ttgccttgct gttaatat
//
LOCUS       DAWAOG010000253         2857 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4513_length_2857_cov_1.938615, whole genome shotgun sequence.
ACCESSION   DAWAOG010000253 DAWAOG010000000
VERSION     DAWAOG010000253.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4513_length_2857_cov_1.938615"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1383..1392
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          753 a          632 c          684 g          778 t
ORIGIN      
        1 gtatatggac cgcttacaga tatgacagta aatgagccgt ttataagaga ggagaaaaat
       61 ctgtgacagc aggcgctaac cgcagtgtaa tccacactgt aacagtcggt gacaagaaga
      121 tcgtctatga gcttacccgc aagaaagtga aaaatataaa tctcaggata aagcctgacg
      181 gctcggtgca cgtttcggcg tcaaatgccg ttactatcgc tgtaattgag cgctttataa
      241 gggaaaaggc ggactttatc ctgtcggcga ttgataagta tagcaagcgt aataccggga
      301 gcaaaataca gtttgtgagc ggggagaagc ttccgatact cgggagagag cgcacgctgt
      361 atgtggtcaa ggcaacacat aattttgtaa cggataagga cggaaatata acgctgtcgg
      421 taacggatac agacaactat gagcttaaat ataaggtgta cattacttgg ctaaaggcac
      481 agtgttttga gataatgaca ctgctgtgca aggcggcata tgatgaatac taccgctcgc
      541 tcggaattaa atttcccatg ataaaggtga aggatatgcg ctcaaggtgg gggagctgta
      601 taccgtcaaa ggggatactc acattcaatg ttcatctgac ggaatatccg cttgccgccg
      661 cggaatatgt agtcgcacac gagtttacgc acttcttgca ggcaaatcac agtgcacggt
      721 tttatgccga gcttgcaaaa ttcatgcctg actaccgcga aagggaaagg ttgttgaaag
      781 ggtgactgca tatagctgtg cggggcttga taaggaatat atgaatttaa ggggctgtga
      841 tacagcccct ttttatcatt tatagtttac agcaccttta taagctcatc ataaatcggc
      901 gcatagtgtg cgtctgttat tccgaaatcc atttccttat aagaccatac ggcacgggcg
      961 atgtgatact tttcaaaggc ttcgtttatc attctgaacc agttcagagt accctcggta
     1021 tcagcctgat cgataactcc gtattcaccg cagtaaagag gaagatccag cttttcggca
     1081 acctttatgg catcgccgaa catctttaca aagaaaccgc tgtctatcat acctgaaacg
     1141 ccgtcaaagt cagaagcata atcattgccg aaatactttt cgctgtcggc actcagtttc
     1201 tcgaccgtat cggggaaagt cgtgcggtag gaaaggtcga gcctgtcgat ccatgatgca
     1261 ccctgatgtg tgaaaataag agggctgtag cagtggaagg tcagcacgat gttttcgtcc
     1321 cacggaatgt caaggagcgt cagcccgtaa atgctcgagt tgaagatacc gccgatgact
     1381 atnnnnnnnn nntttcttat ctcctttacg gtacgtcttg ctatcttgtt ccacgaatct
     1441 gtgaaatctg cgcttgtgat ttcgtttagc agttcgaacg ctatatgact gtcatttccg
     1501 taacgccttg ctatttcctg ccacagatat acaaactgat cctgcagctt ttcgttaccg
     1561 aaaaatccgc aatagctcgg atcgtcaaag atatatccgc aggtcttgtg cagatcgata
     1621 acgatattaa gtccgtgctt tttgcaccag ccgacacagc tttcgatata agccattccg
     1681 ctttcgataa gcttgccgtc gtctgtctgg atcacgttgt agtcaacagg catacggacg
     1741 tggtcaagcc ccatggaggc tatcttttcg atatcctttt cggtgatgaa tgtcgaatag
     1801 tgctcatcat tataattctt gccgcactgg gatatccaac cgccgaggtc gatgccttta
     1861 taaaatccgt caaagtttct catagttttc ctccgtcata attaaagttt ccggcagggt
     1921 cgcccctgca ttttatgttt gcgacttaat tataacagcg aaagctaaaa tgcaatatct
     1981 ataatgtggc gaattttata tttttgtgtg attattttct gctgagcgct ttgttaaggt
     2041 cgctttcggg agtatcggta ggtgtgatgc tgtaattctt tacaagcaca tcaagaacac
     2101 cgggtgaaac gaacgcggga agagtaggac cgagaaaaat attctttatc ccgaggtaca
     2161 ggagagtaag cagtatgcag acagctttct gctcatacca tgacagaaca agcgtcagcg
     2221 gtaaatcgtt taccgaacag ccgaaagcat cggcaagcgc aagcgctatc cttattgcac
     2281 tataggcatc gttgcattgt ccgcagtcga gtatcctcgg tattccgtct atcgtaccga
     2341 gatcaaggtc gtttatacgg tactttccgc aggcaagcgt taaaataagt gtatcgggcg
     2401 gggtcgcctt tgcaaagtcg gtatagtagc ttctgctcgg gcttgcaccg tcacagccgc
     2461 cgataaggaa gaagtgcttt atcttaccct gctttacaag tgctactatc tcatcggctc
     2521 ttgacaatac ggcattatgc gcaaagcccg tcataacagt gtgaccgccg ttcatacctg
     2581 tcatttcctt atcctcgtca tagccgccgc attcaagcgc ctttgctata acgggtgaaa
     2641 aatccttgtc gtcgcctatg tggacaagct cggggtagga tacgacagag gtcgtgaaca
     2701 ctctgtcgct gtagctctgt cttaccggca taatgcagtt tgtggtgaac agtatcgggg
     2761 cgggaatgtt gtgaaactca gactgctgat tttgccagcc tgtaccgaag ttgcctttta
     2821 aatgcttata ctttttaagc tcggggtagc cgtgagc
//
LOCUS       DAWAOG010000254         2847 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4526_length_2847_cov_2.153295, whole genome shotgun sequence.
ACCESSION   DAWAOG010000254 DAWAOG010000000
VERSION     DAWAOG010000254.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4526_length_2847_cov_2.153295"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          848 a          623 c          633 g          743 t
ORIGIN      
        1 aaaaatcgca aaaactattg caattttaaa tcggacgtgc tataataaat cgtattgtga
       61 aatataacta tgattatatg tagatctgaa tagaaggagg tgccgtaagt gcctaacatt
      121 aaatctgcca agaagagagt tttagtatct gctacaaagc aggctcgtaa caaggctgac
      181 aagaccgctc ttaagactat tctgaagaag gttgacgctg atactaacgc tgacaacgtt
      241 aaggttgcag ttaaagcagt tgacaaggca gcagcaaagg gtattatcca caagaatgcc
      301 gcagccagaa aaaaatctca gcttgctaag aagttaaacg ctgtttcctg agaataacga
      361 tttaacgtag cgccccccac gggggcgttt tctttttgtg ctttaaaagc atcggattgc
      421 attattatgc tccgatgctc tttttttatt cctttacgct tctgacggta tagccgtttt
      481 cctgccagta atcaattaca tcggcaagta ttgccgtgtt ggtttcggac actgcatgga
      541 gaagatagat ggcaccgttg tgcgttgcgg aggttatgcg ttcatacgcc tttgttctgt
      601 cgggctgatc gtcaacgttc cagtcgttat aagcaaacga ccacaatacg gtggcgtatc
      661 ccgcattctt tgccgccctg agcgttctta ccgagaactc accacgcggc gggcgcatag
      721 tactcatcct gtatccgccg aagttttcac tgacataacg ctgaagtccg ttaagctctt
      781 caatcacttc ttcgttactg atatccggta aggacgggtg tgtgcaagtg tgatttccta
      841 cctcgtgacc ctcgtttatc atacggctga caagctcggg actgctcttt acatagtcaa
      901 gcgtaacata gaatgtagcc tttacgttct ttgattttag gatatcgagt atccgacctg
      961 tacagccatt ttcataccct tcatcaaaag tgaggtatat cgtcttgtca tccttgctgc
     1021 ctataaatac cccgccgtat tcaccgtact tttcctgagc ctgaagtgag ccgacaggac
     1081 gattttcggt gtcggtttcg tatccttgtc catagcacac tttttcggta ttgtccgcaa
     1141 gaagagccgc ctcggcaggt gtctgacctc ccctgtcagt ttgcccggag ctccgacctg
     1201 ttgtggtttt ttctgcggta cagccgcaca taaatatcac cgccgttata accgccgcca
     1261 gttttttatg caaaaaaatc acccctttac gaaaatagta tccgtaaaag agtgatcgta
     1321 attcattgta attattggtg tcagttgccc agatatgtct ttaccatagc ctgcaaaagc
     1381 tcatcacgat ctatatgacg tatgagactg cgggcattgt tgtatgtagt tatataagta
     1441 ggatcttcgg acaaaatata gccgacgagc tgatttatag gattatagcc tttctccttg
     1501 agcgcgccgt aaacggtagt caatatttcc ttcatctcgt tttctctttc gtctttgagt
     1561 gaaaacggca ttgttttatc gatcataaag tatcagtcct ttcgttaata tcttcaaaaa
     1621 accgaaataa ttttaaggtt gtttatacta ttatacacta caaaccgata aaatacaata
     1681 gcgggcagca aattttcgtt aaaatcacca atagcggctc tcatttttgg gtaattgata
     1741 aaactttaca aatcgctatt gaattaacga agtatttttg ttataataac aataaccaga
     1801 ttttcagggg gtaagataat ggctgaacag aaaaaaccga cttcatcaaa cggtgaattt
     1861 aaaacatcgc ctctaggctt cgacaaaaac gaagtaatgg catacatagt acagcataac
     1921 aaagagcgca aagcccttaa ggaagaaaac gacgaactga agagagctct tgaggaaaaa
     1981 aagaaggcaa acgaaggcgc agacaatgag cttacagcag agcttgaaaa caaaaaagcc
     2041 gagcttgacg cgcagatact tgaaagcaga aaacaagttc tcgatgaaag acgcaagctt
     2101 gcacagaccg aaaaggagct ccatgtcctc caggataagc attccgctct gacagaagag
     2161 tttgccgcat tcaaaaagca ggcggcaacc aagatagaca aggcaaagca cagcggcgga
     2221 acaatagatc ccgcacagct tgccgagata tccgccaagg caaacgccga tgcaaatgcg
     2281 gttatcgcag acgcgcagaa aaaagcggaa gagatcatca cagccgcaaa ggcatatttt
     2341 gccgatactg ttcagaaaac atatgattac aaacaggcgg ttcttgaaaa agccggcgct
     2401 ttcgccgaaa atgtaacgtc aagcggagct gttgctcctg caattgatat tgccgctata
     2461 cttgctgctg taagcagtgc tttaaataat gcgattgcaa atgctcaaag cgccatcgcc
     2521 gaagcaatgg acgaagcaaa aagcaaggct tccgatgagg taagcgctgt aaacgatgcg
     2581 gtttcaaagg ctgttgaaga agctaagagc aaagatgttc cggctgctga agccgcagat
     2641 atcccggaag aaaagctttc cgacaacgaa gagctcagtg ctgcgaaagc cgagcttgag
     2701 aatatcggcg gctttgacga aagcgagcta aattcatatg ctccgatgaa tgttgagcct
     2761 tacagctttg aacttaagct tgatatgccg gcacagacag atgacggcga tgatgatatt
     2821 gatgatatgc tttcggacaa taacggt
//
LOCUS       DAWAOG010000255         2843 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4535_length_2843_cov_2.014706, whole genome shotgun sequence.
ACCESSION   DAWAOG010000255 DAWAOG010000000
VERSION     DAWAOG010000255.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4535_length_2843_cov_2.014706"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    896..905
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1856..1865
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          765 a          630 c          554 g          874 t
ORIGIN      
        1 gagcgaggag ctttctggga tcgaagccct taccctgaag atccttacct tcttcgatgt
       61 actttcttgt agcttcctgg aatgcaagct ggcactctgt attaacgttt atcttagaaa
      121 cgccgagtga gatagccttc ttgatcatat cagcaggaat acctgtaccg ccgtgcagta
      181 cgagaggcat attaccaacc ttctccttaa ctgctgccag tgtttcaaat gaaagaccgg
      241 gccagtttgc aggatactta ccgtgtatat taccgatacc tgcagcgagc attgttactc
      301 cgagatctgc aattgccttg cattcatcgg gatcggcgca ttcgcccatg cctacaacac
      361 cgtcttcttc gccgccgata gaacctactt cagcctcaag tgagatgccg agtatatcgc
      421 aagtgttgac gagagctgtt gtcttttcaa tgttctcagc gataggatag tgagaaccat
      481 cgaacattat agaagagaaa cctgcattga tgcaagcctt agcaccttca aatgtaccgt
      541 gatcaagatg aagagcaaca ggaactgtga tgttcagttc ttcgatcata ccctttacca
      601 taccaacaac ggtcttataa ccgcacatat acttacctgc accctcagat acacctaaga
      661 taacaggtga gttgcactcc tgtgcagtaa gaagtatagc ctttgtccat tcaagattgt
      721 ttatgttgaa ctggcctacg gcatacttgc cttcaagtgc cttgttcagc atttcttttg
      781 cagaaactaa catttgtttt ctcctttgat tcttattacg gccgaaaccg cctttcagat
      841 ttattatact acatctgccc tgcaaatgca agtgaaaatt aacaaaaaat aagagnnnnn
      901 nnnnntattt taccaaatct ctttacattt gtcaaatgtg gattttatat agctgtaatt
      961 cactcccatt catagcagga aaagaaattg tacattattc aaatcacatt ttacagaaat
     1021 tcgctttatc atcgcattta aacgctttta ctacaatttc gacatagaat ttcaaaaaac
     1081 aagaactttt atcatcttaa aagacagtac aaaaattcag caatatgcac atataagcat
     1141 ttactgttac gctatatata gtataatttg atacgaataa aacggtggag ttaatatgcg
     1201 ttatgtaatt tttgttataa tatgcctttc gtttcttttt gcttccgtga ccggtaaaat
     1261 gtcagatctg tcatctgcgg cactgacatc gtgtaaggat gcagtaacac tgtgtgtatc
     1321 cctttgcggc actatctgcc tttggaacgg tgttatgaaa gttgcggagc gctcgggtgc
     1381 tgtaaaaaag ctgtcttgtg tatttgcaaa accgcttgga tttctctttc ccgatataga
     1441 taaaaaaggt gctgctatgc agtatatagt aatgaatttt atttccaatc ttctcgggct
     1501 gggtaatgct tcaacaccac tcggaataaa agcgatgaac gagctgaaaa aagaacaaaa
     1561 agccgataag accgctacgc gcagtatgat catacttgtt gtgatgaaca cggcaagcat
     1621 tcagttcttt ccctctacta tagtggcaat cagggcttct tacggaagtg catcttcgtc
     1681 agatatagtc ccctgtgtat ggatagtttc gacaattgct ctcggcgctt cggtaatatc
     1741 cgtttttgcc gctgacaaaa tatcatcacg gagaaagaaa aatgactgaa ataacggatt
     1801 acatagcgcc tgctatgata cttgcggtat tcacatacgg aatagcagtc aagacnnnnn
     1861 nnnnntttat agaaggcgca tccgaaggac ttaaaaccac gttcgacata ctcccggcac
     1921 ttatatgtct tatgacctgt atcggtatgt tcaaagcatc aggttgtatg gaaatgctga
     1981 cacagcttat aagcccggta acatcttttt tcggctttcc ggacgaatgc atacctctga
     2041 tatttattcg cccgctgtcc ggcagcggtg cgttatctgt atatgacagt ataatcgcag
     2101 aaaacggtgc tgacagcttt gtcagtcgtg ttgcctctac gatgatggga tctaccgaaa
     2161 caacattcta cactgttgcc gtatacttcg gagcggcaaa gataaagaaa agccgctatg
     2221 ctgttcctgc ggcgctgaca ggcgatatga taggctggat cgcttctgcg gtcgcagtaa
     2281 ggcttttctt ctgatataaa aaaagcacgg tgaaacagta ttccgccgtg cttttttgat
     2341 atcaatcttt tacaatgtcg cacacttcgg ctctcgtgac ctttatcagg tcatcgggag
     2401 cgattattat ctgcgtacca agcttaccgc cactgactat aaccttatca aagtctttta
     2461 tcgtatcgtg ataaactgtc ttatactgct ttttcattcc tatcggtgta cagccgccgc
     2521 gaatataacc tgttaccgcg ttaatatcct tgacgtgtac catttcgatg gacttctcac
     2581 cgaccgcctt tgccgctttt ttcatatcca gttcaagcgc tatcggaata gcaaatacat
     2641 agtagttttt agttttaccc tcggtcacaa gtgttttaaa agtgctttcg taaggctgtc
     2701 ctaacatatc cgcaatgtgt acgccgtcta taaattcgtc acactcataa gtgttgactt
     2761 catagtttat cttatttttg tccagtatgc gcattgcatt ggtcttgttc tctttttctt
     2821 ttgccattgt aattctcctt tgc
//
LOCUS       DAWAOG010000256         2833 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4564_length_2833_cov_2.025558, whole genome shotgun sequence.
ACCESSION   DAWAOG010000256 DAWAOG010000000
VERSION     DAWAOG010000256.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4564_length_2833_cov_2.025558"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2020..2029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          809 a          576 c          694 g          744 t
ORIGIN      
        1 gaagaagcag aaaacgtaat caaagcggca gaggaaagcg atcttgcgga taagatcaag
       61 aaataccgac caagaccgtt aagccgtgtg gtttttgtat gcgatgaaac gatgtattcg
      121 agcaacctga agtttgcaga cggcactaat cttaatgcag aatgcaaggt cagcgaaaat
      181 gttaaggatt tgttttatgc tctggcagaa aagtacggag aggaagaggc ttcctgattt
      241 tgcagggata acactacagt ctgctatatt gttaataaga tatgacgata gtcaactgcg
      301 taaatatgca aacaataatt ataaggtaaa aaagctgata agcttaaata aagatgaaac
      361 aggaggaata taatgggact ttttgacaga cttaaaaaca cagcggcaag cgctgtagga
      421 ggtgcggtaa acaatgccgc aatgtctttg ggcaataaga gcgaaacatt tacattcacg
      481 gcacttccgg aaagtcttgc acaaatgcag gcactgcctg aggcaaatct tgattcgccg
      541 ttcaaaactg ccgctcttac agtatgcgca ctttgcgctt atgcggcgga caaaaatatc
      601 ggtacggaaa tgctcaactg gctgagagga ccgcgcccgc taaacggcgc tgaaatttca
      661 ttcctcaatg accgtttccg tgacggaaag acctatatcc cgttctcata ttttgaggga
      721 gcaacaccgg ataatgatta cacgccttca gaaccgttca gagtaacagt gcagagcaca
      781 cacgtttcag gcgaggagca gggctatatg aagctgttta ttccttgcgg cggcgcagac
      841 agcccaagac ccataaaact gcgtatgaag ggcgacggca aatggttctt atgggagcag
      901 tacctgctta caggtatacg cacacccaaa tccgcagacc cctgggcata agctgtaacg
      961 gcttatgttc gcataaatca ggaaaattca gagtatgaaa ggatgaatag tatgaatctt
     1021 attggtaaat ggaaagttaa agaattgccg gtttatcccg aaccgggtaa aatgattttc
     1081 gtagcggttg aagacttccc gaagtatatc actgacgagg atctgcttaa cgattatatg
     1141 cagcaggctt cttttattta cgaattttgc gaagacggca cggttgaaat aatgatgcct
     1201 attccggaag aaatgatgga aaaggcaaag gagcagggag ctaagataaa gggtaactac
     1261 ggagtgatcg acactaccgt gtggaaggaa gaagacggta agttgttcta tgatacaaaa
     1321 ataaacggta ctgttatgga cgagcctgtt tcttcatttg atgaaataaa ggaagcggaa
     1381 gacggcacta tccgttttaa cgcaggcttc accgttttag aaagagtatg atctccttgt
     1441 tgatcgggtg agttaccacc gtatgctgcg gcatacggtg gttcttttta taaatctccc
     1501 ggttttgtct tgaaatcttc ccgataaagt gctataatca tacgtcgagt gatcaagaaa
     1561 agaaggttat tttttggcag aaaaagcaag atccgtttcc cctgaatttt tcggaacaga
     1621 aagcgtgtgg aagataatgc tccgtatcgc tccgccgata atgctggctc agctgataca
     1681 ggcaatgtac aatataatcg atagtctgtt tgtcggacag tattccgatg acggattgac
     1741 cgccctgtcg gctatctatc ccgttcagct tatcataata gctcttgcgg tcggtacggg
     1801 agttgggacc aatacgctta tggcaaagca gtatgcgctg agggacgaaa aaggcgcaga
     1861 tcatacggca ggtgtcggaa ctgtgctcgc agtgataatg tgggcgctga tctctgtaac
     1921 atcaatggtg tttatgggat cgtacgttgc aatatcggct cagtcgcccg ttgcaatgca
     1981 gtacgcaaac gaatacggta tgatagtaag cataggaagn nnnnnnnnnc aatatgaaac
     2041 tgccgatgat cgctcagatt gtcggagcgg taacgaatat cgttcttgat ccgttgctta
     2101 tctttactgc aggacttggg ataaagggtg cggcaatagc taccgttaca ggtcagatag
     2161 ccgccgcagt tatcgtcggt gtaaaaggcg cacgaaagcc gcccgaaata aagcgtatcc
     2221 cgtatatcgc caaaagcata tacaagctgg gctatccgtc aatatgtatg caggctcttt
     2281 acactgtcta catagctatc cttaacatta ttcttgcagg attctgcgat gaggcggtga
     2341 ccgtactcgg tctttactat aaattacaat cgttcttctt cataccgctt ctgggattgc
     2401 agacgtgcat cgtacctgta ctcagctaca attatacatc gggagattac ttaaggtgca
     2461 gaagaatctt ccgcagttca ctgataatat ccggcgtatt tatgatactc ggtgtgctgt
     2521 cctttgagct tatccccact cagcttatag ggctgtttac tcagagcgaa acggtaaaaa
     2581 gcataggcgc agtgggtttc cgactgatag gagcaagctt tatccccgcc gtattttcgt
     2641 ttatgacacc ggttttcttt caatcgatag gttattccaa gaccagtacg ttcctttcag
     2701 tattacgtca gcttatctgt ctggtaccgt tattctggat attctcgttt atcggtctgg
     2761 attacgcatg gcttgcattt ccgctgaccg aggtaattac aggagctatc ggtatgggaa
     2821 tgtactatta tgc
//
LOCUS       DAWAOG010000257         2827 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4579_length_2827_cov_2.470779, whole genome shotgun sequence.
ACCESSION   DAWAOG010000257 DAWAOG010000000
VERSION     DAWAOG010000257.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4579_length_2827_cov_2.470779"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1575..1584
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          636 a          734 c          568 g          879 t
ORIGIN      
        1 gtatttcagc atctgattat ccgaaagcag atttttaagc attatgtttt ctgataacgg
       61 actgcttatg tcccgatcat acgccaacat ctcagtaatc ttcattcccg tagcaagctt
      121 tacagatgtt gcatttcctt cactgcttat agattccttt acagccagat atctcacagt
      181 tacatttgca ccgtcactta cagtgaacct tgtacctgca ttgttaaaaa atgtatctgc
      241 ttctatattt gcgttgtcgt caattgtgaa cgtggtgtta ctgtaagcgt atacattatt
      301 gatataacct gtacctgtta atgtaagctc accgctatta tccgtgctgt ttttctcatt
      361 gatattaagt ttgtcatcca gcctgtgacc gttaaggtca agtgttacac ggtattttac
      421 aatatactcc tgactggaat ctttctcaat atcctgatag atcttaacat agtttccggt
      481 atcggcagta atgctgttaa ggcactctgc gagcgtgcca tagcctgtcg gcgcactgcc
      541 gtctgtgctg attgtagcaa cgaactgatg cccacacaca catatacctg tgtccgtgtt
      601 gatattctta tgatcgcagg cattgtgaca gatattgcag acttcatctg taaagccgcc
      661 cgtatgcttg cacttttcaa atgtgaaagc aaatctctct gttaatgtat atgcctcgcc
      721 gttgtcgttg ttataaacaa catcgcagta aatgacatag ctaccctcag gtacattgtc
      781 ggagaacaca agcgtttcta ctccgtctac tgtggttact gtgccgaggt cggctatatc
      841 atacttcttg ctgtcactgc tccagttgta ctgtaagccg tcttgatttt cataaccggc
      901 ttcaacatca taccgaagcg ttatcttctg ctcgccgttg taatttgtat agtatttgct
      961 ctgtgaatcc ctacttgtaa gggcaaagtg gggcttacct acagttacgg taaccgtatt
     1021 tgaataaacc tcgccgctct ttacacgaag ctcatactga cgcgataaat caggctcatc
     1081 gcatattacc tcgcagacgt attcgccgcc tgtttttgtt atgtgtgcgt caatattcgc
     1141 actgccggga gatgttaccc attctgcctt gggatcaaat gctccgtccg caaagttctt
     1201 cgcagttata tagaaatatg cagtggaagc gttgccattc tcgtccgcat atactgcgcc
     1261 tatctgctta ccaagcttaa tagaaggctc tgtatcaggc tttataacta ccgtcttagc
     1321 gtccgcttcg ccaatataat aatagccgtc ggggatcttg acctcgcctg tcacagcact
     1381 ttccgaagcc ttgaactcgg tcgagccctt tactgtaaat ctgtcctcgt ctattgtcgg
     1441 tacagtttga ccgatagcgt atgaaccgcc cgtagtatat accgagcctg ttccgctcat
     1501 tataaggttt actttgcaaa tgccatactt agtaccgtaa gcagtgacct taccgccgct
     1561 tatttcggca tccannnnnn nnnncttgct gcattgcctt caggcacagt aatactgccg
     1621 ttttcgctga cggaaatttt gtcgaaatta ctagtaagca atattgcact gtttaacgta
     1681 gattcattag cacaacctac tgcagtaaga ctgccgttgc cgtcaacagt aatgccgccg
     1741 ctgacattga cagccacacc ctcaaaagat gcagttgtcg catttgcgtt cagcttaccg
     1801 tcattatgta tttcaagcga atttgtcttt acagtacctg ttgcgtttac tgtaccgccg
     1861 ttcaggatta ttgatccgcc gagagagcgc gccatactgt ctgtagagcg gttctctgca
     1921 ttcagcacac cgtcgccgct gatcgtcatt gtaccgtcac agttaacatc attcgtattt
     1981 acggcactga gaacgtatgt atcgctgtcg ccggtgttcg tctgtacgat agtattgtta
     2041 ccgttcagca ctatctccgt accgtcgggg actgttacga cgctcatcat attctcttct
     2101 gttgtcgcct gaatgttcac tccgttaagc gtcagcgtct ttgtgtcata atcccagctc
     2161 cagccgtcac cgttttcgtc cgccgtcttt tctctgaaat caagcggtgt cgtgatgaca
     2221 ttgctctcgc cgtcatgcgt atgtgcctcg ccctcggcaa atgccgataa cggtacagcc
     2281 gtcagcgtca ttaccgcaga cagcaccgca cttatgagcc gtacaaattt tttcttcatg
     2341 gttttcctcc tgtagatttt cccgtgctta cgccaggcac ctgcacttat gcagtgcctc
     2401 cttcagcttt tcaagcgtta tcggcttctt gtggaaatag gtacagccaa gtctgtatga
     2461 ctgcgccccg aatccctcat cgtccgacag ccatattaca ggcgtgtccc tatcggcatt
     2521 cttcgccgcg ataacgccct ccataccgtc cgctccgtcg gcaagcacga ttataaggtc
     2581 agccgtctta cactccttcg caaagctctc atacctctga cataccctgt aggcaattcg
     2641 tcgatatgcc gccgttgcga tctgtgttat tttcgctttc agtatctccg cctcgtttgc
     2701 ggtatcgtaa acgcagatga gcatacctct cccccttata attttataat tatatcataa
     2761 ctcattcgct tgacattttc aactttttgg gcacgaacgg ttgatatagt ggcgcaaacg
     2821 gtaatga
//
LOCUS       DAWAOG010000258         2825 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4585_length_2825_cov_2.358845, whole genome shotgun sequence.
ACCESSION   DAWAOG010000258 DAWAOG010000000
VERSION     DAWAOG010000258.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4585_length_2825_cov_2.358845"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          790 a          560 c          656 g          819 t
ORIGIN      
        1 cggtgtattg cggcttattt caatgtagac ggaaactata cggcggataa ccggtacctt
       61 gtagtactga accactgcac atataagccc tatgatgatc gagaatatta taccaagaac
      121 accgagcttt acagtaagca gtgcggcttc cccgaacagc ggcaggtact cagcgatagc
      181 gttaagatcc ataccgtcac atcctttcaa tttactgagc cgacggcaaa ataaaagccg
      241 gggctgttac gctcccggac aaatggcagt cgccgcagtg cttttacggc tgtatgacaa
      301 caatgccgct gcacccaaat gccattttac ttgtccggga gctgatacag caacacatta
      361 ttctgttgtg cgttgatgaa atagtagcgt acatcgtatt tcctccgttt gtttcaaaac
      421 ttataattct catcggtttt gttccgatga tgctattata ctacctaaaa cctactttgt
      481 caataggtta agagtgtttt attgaaaatt tttttggagg tataatccaa tgtatgcggt
      541 tttcgggata atggtgcgga ttaacagggc tgccgcccct cgaccccgtt tggttttcaa
      601 cagtgtgctt aacatacaaa aataattata gtataatacg gggaaaaatg caagtaaaat
      661 tttcgggtga aaatcacggt tttttgtggc agaatgtaaa gttgtgatat gcctttacaa
      721 cacagcgaaa aaatgctaaa ataagtataa ctctgttacg aatacgaaag gaattacggt
      781 tatgaaagaa atatcggtag aagaactgaa attaaatccc tccacgctta tcggcaagga
      841 gtgggctttg ctcacagcag gaaacaagga cggttataat acaatgacag taagctgggg
      901 acagatgggc gagttgtgga acaagcccac aacggtagtc tatatccgcc ctcagagata
      961 tacaaaggaa tttgtggata agtccgatta ttacacattg tcattcctcc ccgacggaca
     1021 cagggaagcg cagagctatc tcggctcgca ttcgggcaga gatgaggata agcttgcaaa
     1081 gaccgacctt acaggcactg caacggacga ttacgcttat atagagcagg caaagctcgt
     1141 gcttgtctgc aagaagctgt acagacagcc catgacgccc gattctttta tagacaagtc
     1201 gcttattgaa aagtgctatg ccgcaggcga ttttcatgag atgtatgtcg gcgagatagt
     1261 gaaagtgctt gttagtgaat aaccgatgaa aaggacagtg gtaataggct gttcgggttc
     1321 gggtaaaagc gtgttttcga gaaagctccg tgacgcaaca ggattatcgc tgtactatct
     1381 tgatatgata tggcataagc ccgacggcac gaacatttca cgggaagaat ttgacaaaca
     1441 gctcgacagc ataatatccc gtgacagctg gataatagac ggcaactatc agcgcactct
     1501 tgaaacaagg ataaaggctt gtgacacagt gtttctgttt gacttgccga ctgaaatctg
     1561 cgttgagggc gcattgtcac gcataggcaa aaagcgtgag gatatgccgt ggtttgaaaa
     1621 tgagcttgac cctgagttca gacaatggat agaaagcttc agggcaaatc agatccccga
     1681 agtatacaga ctgcttgaga aatacaaaaa caacagggag atagttgttt ttcgcacaag
     1741 agagcaggcg gataagttta ttgaaaagct cggataacat aaataaccca ctgtgatttt
     1801 tacacggtgg gttatcttta ttcagcgtta attatcagcc tctggtgttc ttgaaatctt
     1861 cataaagtcc gtatcttgca atcgtagacg ataccttgca gcgaagtccg ttatcggtat
     1921 tttcaaattt gtacgcgcac agggcatatg tgatctcatt gtttttgtca acgctggtgg
     1981 gaatggcggt tatgcacata atgtcgttgt tgttttggtc gtcgtagaac agcagatatt
     2041 tagcctgata gtatctttca tcgccgcttc ctttttcgcc gaatgcggcg taaatgcaca
     2101 gcttgccgtc atattccgca tattgcttag gaataaaatc cttgaagttg tcgtatgtga
     2161 agcctgtgta aagctcgctt gcctttttat aaaagccttt catatcggaa aatccgaaga
     2221 aatcggggtt tgcctcaaga tagtttccgt agacggcatt cttctttata aattcatcta
     2281 tttccgctct ggtggcattg atcatttctt ttccgttttt tatagcttca tagcctgagc
     2341 agaaatcaac cttttgatca aacagtcgtt gcctggttac acggtcgatt atttcgttgc
     2401 cgatgctttc gtctgtgcag agcttcagat catcaaaatc atcgctcagc gaaattgtct
     2461 ttccgtcata agtgatactc ccgaatttgc tccagaaaga tttgtcggtg agaacctcgg
     2521 cttttatgct gttgatgtcg atctgttctt ccgagctgtt gttactgttt tcgtcggatt
     2581 tgttactgct ttcgacagat tcgttactgc tttcgtcaga tgaattatcg gtagtagaag
     2641 taccggaggt tgtaggagta ctgtccggag cggaagaagt gcttgcgttt gaagagcaag
     2701 cgcaaagtgt tgttgcaata agaaatgctg atactgtgag aatgattgat tttgttttca
     2761 tggggatacc tccttaattt ttctgatttt attatatcag aatatcgtac aaatttcaat
     2821 aagaa
//
LOCUS       DAWAOG010000259         2819 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4595_length_2819_cov_2.438857, whole genome shotgun sequence.
ACCESSION   DAWAOG010000259 DAWAOG010000000
VERSION     DAWAOG010000259.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4595_length_2819_cov_2.438857"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    814..823
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          844 a          597 c          662 g          706 t
ORIGIN      
        1 gtatcgccgt atgtatttcg ggcggtaagg attctatgct gatggctaag ctgttccagg
       61 agcttcaccg ccacagcgac tttccgtttg aggtaaagtt catcgtaatg gatcccggct
      121 acagcatagc aaacaggaat gttatcgagg aaaacgcgcg aaaactgaaa ataccgatag
      181 agatatttga gtcggacatt tttgattctg tctacaacgt tgaaaaatca ccctgctacc
      241 tttgcgcaag aatgagaaga ggtcaccttt acaactatgc aaagagcctt ggctgtaaca
      301 agatagcgct cggacatcac tatgacgatg tgatagaaac gatacttatg ggtatgctgt
      361 acggcgctca gatacagact atgatgccga agctccacag cacgaacttt gagggtatgg
      421 agcttatacg tccgctgtac cttgtgcgcg aggacgatat aaaggcgtgg agggactaca
      481 acggactgca ttttattcag tgcgcctgca aattcacaga cacctgcact acctgcaaca
      541 acgaggaaac acgctctaag cgcgtcgaga taaaacagct tattgcaaag ctgaagcaga
      601 caaatcccga ggtcgaaaag catattttca agagcgttga aaatgtgaat atcgatactg
      661 tcattgcgtg gaaaaaggga ggagaaaagc atcacttcct tgacagctat gataaaataa
      721 tcagactgta aaaaattatg tttttcccaa aagcgcttgt tctcaatccc tatccccaaa
      781 cccctttccc caggaaaggg gctatattac tggnnnnnnn nnncgcccct agaccctgtc
      841 gggggcttcg cccctgcgac cccatatcaa tatttacaag aggtttttca cagtctgaaa
      901 tcatatgata tttgcatttt ctgtgcggat aaggtaaaat ataatagcaa gctatgccgt
      961 cacggttata aattgtgacg gcattaaatt taaaggcggg aggcggataa actgaaaaat
     1021 cacagacgca ggctgaaaga atttatccgc ctgtttatgc aaagcaagcg cttccgtata
     1081 gtagtctaca cagcaggctc actgacgctg aacgttgcat acgccgcata taacggcgtt
     1141 ctcggtctgt tctacggctc gatatggttt atgatactgg gcgtgtatta cggtctgctt
     1201 gccgtgatgc gttatatgac tgtatcatat gagaaaaagc tggacaaggg catacaggta
     1261 gtatcggaag tatatgtgat gcgctttacg ggtatgatgt ttattattat gagcgtggta
     1321 cttatgctta ttgagctgta cagcatgata cacaacacga cacgacaata cgcaaggctc
     1381 gttattgcaa gtatcgccgt atacacggtg ataaagcttg tccgtgctat cgtgaacctt
     1441 gtgaaggtgc agaagcaccg ctcaccgctt ctcagcgcga taagaaatat ttctcttgcc
     1501 gatgcgggcg tttctgcggt aacgcttatc cgctcgctgt cggtgtatat aacatggctt
     1561 acggaaaatc agaagctcgt tgtcagcaca ttctcaggag tagtcgtata tcttactaca
     1621 cttgtgatgg gcgttgttat gctgctgcgc cataccgatg aaaagcagaa tataccgtta
     1681 atgaccgata acggcgagga cgcttcaaaa atcgatatgg taagggaaaa gctgaccgaa
     1741 aactttgtga ggataagcga caacgtggtc gataaatacg atgagctttc agacaaagcg
     1801 aacgaaaaaa acgaaatgct taaaggacat atcgatgagc acataaggaa cattgaagaa
     1861 aaacggaaaa acaatgataa tagatactga tttctttgta aagtctgaat ttatttgatt
     1921 gctgctattg atttttaatt tcagatgtga taaaatatca atagatttca gcagaagaag
     1981 ggagattttt ttaatgttta tctcagaaga tgtaaaatat gtcggtgtaa acgacaccac
     2041 gatcgacctg tttgaaggtc agtacagagt gcctgacggc atttcctaca actcctatgt
     2101 cattcttgac gaaaagacgg cggttatgga tactgtcgat ataaactttt cggcaatatg
     2161 gcttgaaaag gtaaaaacag agcttggcga cagaaaaccg gattatctgg tggttcagca
     2221 tatggagccc gaccactctg caagcattgc ggagtttctg aaattctatc ccgacacaac
     2281 ggtagtcggc aacgcaaaga cattcacgat gataaaggga tttttccccg accttaccat
     2341 tacaaacacg cttaccgtaa aagagggcga cacgctggcg ctcggcagtc acacgctgac
     2401 ctttgtattt gcccctatgg tacactggcc cgaggttatg gtaacatacg actcaaagga
     2461 caagatactg ttctctgcgg acggcttcgg aaagttcggc acacccgaca ctgacgagcc
     2521 gtgggaagac gaggcacgca gatattacat tggcatagtc ggaaagtacg gtgcacaggt
     2581 acaggcgctt ctcaaaaaag ccgctacgct tgatattgaa aaaatctgtg cgttacacgg
     2641 tcctgtgctg agcgaaaacc tcgcacatta tctcgatctt tacaacaagt ggtcgtccta
     2701 cactcccgaa aaggacggcg ttatgatagc ctacgcatca atctacggca acacaaagaa
     2761 agccgcagag cttttatatg acaagctgaa ggcaaagaat gttgatgtta ttatcacag
//
LOCUS       DAWAOG010000260         2815 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4607_length_2815_cov_2.140942, whole genome shotgun sequence.
ACCESSION   DAWAOG010000260 DAWAOG010000000
VERSION     DAWAOG010000260.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4607_length_2815_cov_2.140942"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          744 a          590 c          600 g          881 t
ORIGIN      
        1 ggtcagagtt gcgaaaatca caaacggtaa cactactacc tttaggaaag cgggacttgc
       61 taagaatacg gcttacaagt tcagggtaaa ggcttactat atgagcggta agactgcact
      121 ttatggtaat tacggaagcg taagcgggag gactgcggct aagtaatacg attttacggt
      181 aacaaaaaaa tcaggcatcg gtgtaaaagc cgatgcctga ttttgtaatt gtaatccggt
      241 caaaccgtgt ttaatgatat cggttgagct ttacgttact tctgtcataa tatcgtcaag
      301 ctctgtgcta tccgaacctt tgaacagtat agcgtcatta tcacggagat tgagcttaag
      361 aaactctgtc agagcttccc gcgactttaa tttaataacg aacttgttct taaggtcggc
      421 ggtttccata agctctgccg agatgtcgcc gtagcaaacg gtaatgttta catttgcttt
      481 attgataatg ttgcctacct tacggaaaat ttcaagctcg tgctcatcac cgtcgccgac
      541 ttccgataat acggcaattc tgcgtgcttc cggaggtagc ggaagagtac agagctcttt
      601 cagtgccgag ccaacacttt cgaccgcatc attttcaaaa tccgaaataa catagatccc
      661 acgttcgttt tttacggtat taagtccgtt tgagtggcgg tatttttcta tggcagggat
      721 gatctgctta ggcggtattc ccattatctc gcccagcgca aatgtggcaa gcgcctgata
      781 aacgctgtgc ttatcatcgg aatacagctc tatgtgataa ttatccgtgc cgtggattat
      841 atcaaatatt atcttgtcct gcaaaagctg aatgttatct gcataataat cagccatttt
      901 gttgtcaaca ccgatagtga ttatatcctg aggtattgtg aatattccgg caagattttt
      961 atcgtcgata tttatgataa ctgcaccgga ttcgttcatt ccgccgcata ctttcagctt
     1021 ttcgtcaata agctccggct ttgtaaggtc gcgcggattt ttggaaacta cggtacttgt
     1081 aagcacggct atatcaaaat ttgccgtctg tgaaacgcgc tcaacgttac cgccgttcac
     1141 acacgaaacg tttataaccg cagcttctgt tttctttgtc gccgagaaca tcgctctcag
     1201 catcgaagcg tagttgtttg taacggaaac agaacgtact gtttccatct tgtttgaaag
     1261 catctgattt atcatgtcga gcggatcgat cttttcggga ctatccatca ccgctattga
     1321 ccacatacca agctttgctt ttatgaatac tgcagtttta ccgagcgcat cttcaagatt
     1381 gcttacgact atattcggta tcttgtctat agattcatcg gtgataacta cagccgctcc
     1441 cttttcaaaa gctgatatgg cggaagcttt actgtctgca aaatatgcac agccgggttt
     1501 tatcttctgc cgtccgaatg caaacgatgc aattggcaca tcggcggcat tacgaagctt
     1561 tgcgttgagc acttcacaaa gcttgcttac agtagtgatc gaattatcgt tctctttgtt
     1621 ttgttcaccg ggctttttgc cgctgagcga ttcgtaattg tcaaaagtga aatcgagcag
     1681 tgttattgca tcgtagaaca catacttcat ttgcttggta ttttctgcac cgaggataat
     1741 ggcgagtatt tcttcaccga attttcctgc gggaggaaga gctgagcagg caaggcagga
     1801 ctttgagcgg gcggtatatc cgcttttaat gccgattgcg gtttcgtatc tgtatcttct
     1861 gttcctgctg ttgacaagaa ggtttgtatt ctgtatctct cgcggaccgt tgagattggt
     1921 agcaggtatg gtgtatttat ccgatgcggc aatattcctg aaaattctgt tagtcataca
     1981 gtaacggcaa attatcgcaa ggtcatatgc atttgaatat tgttcggaag taaatttgcc
     2041 gtcggcggaa gttattacgg tactttcagc gccaatttct tttgcttttt cttccatcat
     2101 aaccgcaaac ttgtcaattc cgccgcagat aaattcagcc agtgcaaata acgaatcatt
     2161 agccgaaaca agcatcatag agtaaacgag atcaagaaca gatatctgtt cgcctgcttc
     2221 aaggtttata gcagcggcgc ttttccatcc ggaaacctgc tttacaacat tttccgaaac
     2281 ggttacctta tctgtagggt tgcatttttc gagcgctatt atagatgcgg ttatcttggt
     2341 aagaaaagcc ggcggcaatt tcattcttgc gtttttttca agaagtatac gaccttcgtg
     2401 ctgagtaact actatagcag cctgtgcatt tatttcaggc atttctgttt tttgaacagc
     2461 tctgacttct gacattaaga tccgtccttc ctaatagttt taatatagtc aagcaattat
     2521 aaagttgatt ttcatatcaa ttatagcact acaaagctgt aaatgcaatg atttttgcga
     2581 ttattttcac ttaatccgtt acggaaaatg acgaaataaa ttaaagagat aaatttcgtt
     2641 ttcggcacat aaaccattat tttgagcaat cggacgaaaa ttattcagtc tgtcgaaaaa
     2701 cctctatgtt atcaaaaaaa tgggatgtcg agtattgctt gctctgccgg ttttgcatct
     2761 ctgcaaagtc agctaagcat acacagccct tcaccgaggg gaaggggttt gggaa
//
LOCUS       DAWAOG010000261         2802 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4633_length_2802_cov_2.451766, whole genome shotgun sequence.
ACCESSION   DAWAOG010000261 DAWAOG010000000
VERSION     DAWAOG010000261.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4633_length_2802_cov_2.451766"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    335..344
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1650..1659
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          672 a          734 c          606 g          770 t
ORIGIN      
        1 ccgtttgctg ttactgccgc cagcattaca ttttctgtag cgcctacaga cgggaatgac
       61 agcgaaacag aagttccttt cagccctttc ggtgctgtac agtcaagaaa gccatgtgac
      121 tcctctatca ccgctcccat actgcgcaat gccgcaagat gaagatctat aggtcgcgga
      181 cccagctcac agccgccggg atatgacatt ctgcaatgtc ccgtcctgcc cagcactgag
      241 ccaagaaaaa ctatcgacga gcgcatttca cgcataaggc ttaccggtat ctcacagttt
      301 cctattgcag aagaatcgga tatcaccgta ctgcnnnnnn nnnngccgtc gcgcctgcat
      361 tttacgccga tatgcgacag tatgcgaagc gccgcatcga catcggtgag tccgggacag
      421 ttatgtatga tacactcacc gtcttttatc agtactgttg ccgcgagaag aggcaatgcc
      481 gcattctttg caccgtgaag cgcaagctca ccctcgatcc tgtttccgcc ttttataata
      541 agtttctgtt tatccaaaat atcaccctct catagctttt cttcatatta tgccattcgg
      601 ggtgaatggg tgaaatgcga ttttaaagac ccataaggtc ggttatctca cgcagtatta
      661 cgcctgccgc atcgtgcttt gacagctttg ccgcattctc gctcataagc ttcagaagct
      721 cggggtcgtc cgcaagcttt gtgaccgtat ctatgagtac tgcgcctgta agctccttgt
      781 cgtctattat cctgcctgcg ttggcgttct gaagcgtcag cgcattataa tattgatgat
      841 tttccgccgc atacggataa ggtatcaata ttgatgccct gcctatcgcc gtaagctcgg
      901 tcagtgtcat tgcgcccgcc cttgttatga taaggtcgct tgcacacata caagtgtaca
      961 tattatcgat atattccctg atgatgaaat gctcattgtt cgggtcaaca ccgttttcgg
     1021 caaggctttg cataaatgtg tccttgccgt gcttgccgta accgtgaata tgatttatgt
     1081 tgcctttctt ctgctcatat gcaagaagat gcactaccgc ttcgtttatc cttgacgcgc
     1141 cctgacttcc gcccgcagaa aggatcgtaa ggcagtcggg gagtccgagc ctttttcttg
     1201 ccgcagactt ttcttctatc gggatattgt ttctcagcgg gtttccggtg acaacgcacc
     1261 ttttgcattc agagaggtgc ttttttgcgt cctctgtgcc tagcattaca agatccgcct
     1321 ttgaagcaag catctttgtc gatacgcccg gaagagaatt gctctcatgg agcgctgtct
     1381 tgattttaca tttcagtgcg gttttaagta ctgtgccggt cacatatcct cccgtgccga
     1441 taacaagatc gggactaaag ccgtcaaata tctttctaat gcactttccc gcattcagat
     1501 agtagtgcac cgcctgaaaa ttgagcttta tattgtgagg tgtcagcttg cgctgtatgc
     1561 cagccatctt taccggtgca aaatcatagc ctgccttttt gacaagcttc gcctccagtc
     1621 catcgggcga acctataaaa agtatatttn nnnnnnnnng tgcgggattt atatgtcctc
     1681 ccgtacctcc tgccgcaaac gcaacgttca ttgtactctt cctttcggta tcaggtatcg
     1741 gcatctcttg atatgttcag cagcagtccg acttccgcga gctgtgtaat aagcgccgtt
     1801 cttccgtagc tgaagaacgg gagcgaaata ccggtattcg gtatggcgtt gcacgcaacg
     1861 gcgatattca gtatcgcctg caatcctatc tgtagcgtta ttccgagcgc catcagcgaa
     1921 ccgaatttat cctttgctct gcctgctatt ataaagcctc ttacttcaaa cagtacgaac
     1981 agcagtatta ctgtcatacc gccgaagaag ccgagctctt cacatattat tgagaataca
     2041 aagtcattct ggctctcggg aaggtagtag tatttctgcc ttgagtttcc tagtcccagt
     2101 ccgaaccagc cgcccgaacc tattgttata agcgactgcg aggtctgata ggtggtgtcg
     2161 cccgcatcag agaaggggtg aagccagctt gttatacggt ctgtgagata ttcccacttg
     2221 aacacataaa cgagcgctat aaagcctatg accgcaacaa gcacaagtac tataagtccg
     2281 cccttgtttg agccgccgac aaacatcatc acgaatgcaa tagcaagaat aataagcatc
     2341 gccgaaacgt gtcgctgagc catacacagc gccgccgcaa aaaacgctat aaatgcctgc
     2401 ttaccgaagc cgtaccacag cctgttacgg tattcatagc tgttctggag catataggca
     2461 agcaatatta tcattccgat cttcagaagt tcagacggtt ggaattgtat accgccgatc
     2521 gtgatccatc ttcttgcatc gttcaccgcg tcgcctacaa gcaaaaccgc tatcatcagc
     2581 acaacgcaga acagaaaaaa cagtatcgct atattcatac ctttgccgtt ttcaggtttc
     2641 tttttatcgg ttctgaaaaa cgtctttatc gggcgcacaa gcaagtgata atcaaacgat
     2701 gagcatacta tcattgctat aaacccgatc accgccgcaa ccagctgtct ttcggcatat
     2761 gcaaagctgt tgccctcttc cttaagcgca taagcgtaac tt
//
LOCUS       DAWAOG010000262         2797 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4645_length_2797_cov_2.021882, whole genome shotgun sequence.
ACCESSION   DAWAOG010000262 DAWAOG010000000
VERSION     DAWAOG010000262.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4645_length_2797_cov_2.021882"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1128..1137
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          713 a          550 c          660 g          864 t
ORIGIN      
        1 taacaacgat agaggttttt cgacagcctg acagctctca cattaaaatg tgagagctgc
       61 cgggatcgtt atcggttatg ctttttgatt ttcattactg taagcgcgac tgccgcaacg
      121 gtaaatgccg ccgcagggat tgctatgggt gttgttttta cgcctgtttc gggagaggaa
      181 ggctttgcgg ttttaggaat gaccgtttcg ttctttatcg catcgcagtt tttgcaatgt
      241 atcgatttta cgccatcgct tgtgagtgtg ggctgtttgt cgatcgtaaa ttcattatcc
      301 cacgcatgac cgacaggttc tgtttctctt gaagcggttt cgccgcattc gcatactgcc
      361 tcttctgagc ccggctgtgt gcaggtcgga gctttgaccg ttttccaatc agagaatgta
      421 tgctcggtcg ttttcggtat gatcgtatct tcttcggcta tttctttata agcattgttg
      481 tcaccataaa tgaagcattt atgacagcta tcacactgat aataagcaat gtttccttcg
      541 gatgtatgcg tacttgcctt agcttcgtga tagattatct ctccggcgtg acgaccgggt
      601 tcaacttctc cgtatggact gccgcacaag aaacatatcg gcttttgtga gcagatcgac
      661 atactgcgcg gtatgtgttg cttcatcgcc gttcggggta tatgtaaaaa tgtgttcaat
      721 tttcgcttcg actaccgcaa agccgcagat cttgcacagt acaggtgtag tgcagtcgtt
      781 gtcgtcttcg tccgttatgt gcggctcagg ctcctcagag taaatacagc cttcgtttat
      841 acattctctg tagtgtccat actcatcaga ataataataa tcactcattt tgtgctcttt
      901 accgggaacg agtacagttt cacagacttc acagacaacg ggagcggtgc agtccgcata
      961 gtaatcgtta cgcggtatat gctcgctgtt tacctgaacc ttaattgtgt atacagtatc
     1021 atcggagtct gtgacggtta tcgtctgaat tttatcggag ggttcaagag taaagctgaa
     1081 atcctccgac gttatcggat cgccgtcaac cttaaccgcc ttgatatnnn nnnnnnnaca
     1141 gtgcagtttt gcttaaggca tcttacgatt gttttatctg cgtatgatga tatttcagcc
     1201 atatctatta caaaattgca tgtggtatcc gtatcggagg gctcaatatc gaccactgtg
     1261 ctttgtcccg ggacagcaac gattttttct tctgccgatg cgcatatcgt cgatgtcaga
     1321 agcaagggta aggatatcag tacaccgatc attccggtca aattcttgtt tctcatataa
     1381 ttacctcctg actgttatta aatttcctgt tatctgtcgg attttgagga taattctgtt
     1441 attctgattg tatcataaat tttcagatat ttcaagcggt tttactcagg cagaaattat
     1501 atcggataat ttttcttgac agcgtgaatt tatagtgcta taattttgtt agatttctaa
     1561 cagtttgatt ttaaacagag aggtaagcat ggaaaattca cttcatcatt tattgatgac
     1621 agaccatacc gcttttcata agcggatctt ttctgctttg aaaaaggagg ggctgacttc
     1681 gggacagccg aaggtgcttg aatatctttc aaagcatgac ggagcaaggc agaaagacat
     1741 tgccgccgcc tgccgtattg aacctgcaac aatgacatca cttctttgcg gcatggagaa
     1801 aaaagagctt ataacacggt atgcgcccga cagacgttcg ctttgcgtgt atctcaccga
     1861 aaagggaaaa gcgctcgtac cgcttataga aaaggagttt ttgagtatag aggacaaggc
     1921 tacaagcgga ttttccgaga gggaaaaaga aatgctgata gatctgctct cgcggataag
     1981 tgtaaatctc ggttgatatc agaacaaagg aaagtgaaaa tgtcaaagaa agaaactgca
     2041 aaaagatatg ttttatttgt gataagcctg tttatatccg cgctcggagt agcattcaca
     2101 aagcacggag agcttggcgt gtcgcctatt tcttcggtag caaacgttat gagtattaag
     2161 ctggactttt tttcgctcgg aatatggctt ataatctgga actgcgtgct gatactcgga
     2221 cagatactga tacttcgtaa gaagtttaag ccgatacagc ttttacagat accgctgtcg
     2281 ttcctgttcg gatattttac tgacttcgga ctgtggctgg tagggtttat tcctgcggaa
     2341 agctacataa tgcgccttgt aatggtgttt atcggaatag tgatactggg tttcggcgtg
     2401 tcgctgtcgg tttcggcaaa tgttataatg aactcgggag aggcttttgt aaaagcaatt
     2461 tccgatacga taaacaagaa cttcggcaat gtgaaaatag ctttcgatgt aagctgtgtg
     2521 gtgctggcac tgatactgtc gctgttgttc tttgatttca caatagtcgg tacaagggag
     2581 ggtactatta tttcggcact gtgcaccggg cttgttgtaa agctgttccg gaagctgact
     2641 gatgaaccgg tgaacagact ggtgagtgat taaaagcaac atatatttga attgaatatg
     2701 taaaggggtt gtgacacagc ccaaaacgaa aaagtgagtc gaagcgcaaa aaatagtgcg
     2761 ctcggctcac ttttttgata tgtggagaaa atctgtt
//
LOCUS       DAWAOG010000263         2790 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4662_length_2790_cov_1.735649, whole genome shotgun sequence.
ACCESSION   DAWAOG010000263 DAWAOG010000000
VERSION     DAWAOG010000263.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4662_length_2790_cov_1.735649"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1470..1479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          692 a          623 c          598 g          867 t
ORIGIN      
        1 gttcttttcg ttctcataaa tctgctcttc gagtgcgccg agcagttttt tctgcttacg
       61 atacagcata agagtatttg aaacacgctt atggataaca aaactgtcga acggtctgcg
      121 gatataatct gtaacgccga gttcataagc cttgcgtatg tagctttgcg atgtttcggt
      181 ggatatcatt attacaggcg tgtcatcggt ccagtgatat cttttcatca catcaagtac
      241 gccgaaaccg tctactacag gcattattat atcaagcagt acaaggtcaa tatcgtatac
      301 atgctcttta agaatattaa gtgcttcctc accgtttgcc gcttcgatta tatcataatc
      361 agcgctcagc atatcgttga gcatttcgcg gtttatggcg gagtcgtcaa caacgagtat
      421 ccgggcactg tttttctttg gtctgctcac aatatcacac ctcttttaca cggtttgtca
      481 ttttataagt ataaaccttt ttgtgtttca agtcaataga atgtgcttta attattgtag
      541 gtctttcctg attatctgcg ggttccgctt taccgaatgc ttgcagggca gagaaaaacg
      601 tcccatgacc gataaaatca cggggcgctt gcagtattac agtatttact tcagaagagc
      661 tgttgcgtca tccgctatct gcgcgggtgt cagatgagcc tcacgcataa gctcgtttac
      721 atcgtatctg tcgtagaatt tcttggcaag accgaaacac agtgttttta cactgctgct
      781 gccatagaat cgtgcgatct tttcaccgaa gccgccgtca aggataccat cctcgagagt
      841 gatcacaata tcgtgatttt tcttcaggtt ttcgagcgtt tccttatcga tcccggatat
      901 aaaatgagga ttgatgacag tgggtgttat acctgccttt tcttcaatcg cttttgcggc
      961 tttttcaccg agctgataga aagtgccgag cgctattaca gctattttag agccgttttt
     1021 tgtgacctcg tacttgttca gatccgacca gttgttcttt acttccttac cgtctgaaat
     1081 caccggtccg ccgggtacgc gcactgcaac gggatgctcc ttctgctcaa ttgcccagtc
     1141 gagcattgaa aggtattctt cctttgttgc gggagcaaga tatacaaggt tcggaatgtt
     1201 tgacagcatc gggatatcat aaagaccgag gtgcgtaaca tcgctcatac cgtatacaga
     1261 gcctgcaaac acgcttatag caacggggct gttgtttatg cagatatcct gcgatatctg
     1321 gtcgtaggta cgctgtataa atgtgctgta aacgccgtag aaaggctttc cgccacctgc
     1381 tgcgatacct gatgccatgg cggctgcatt ttcttctgcg atgccgacat cgataaattg
     1441 cctgcccgcc tcatctcttc tgcctttccn nnnnnnnnnc gtcataattg tactttgcct
     1501 tgcctgtttc aacgtcaaac ggcatatgcc agtgccagtc ttccttgttt ttctcggcga
     1561 aggacagacc ctttcccttt tcggtcacta tatgcacagc gacagggcgc gtactgtcct
     1621 tgacctgtct gaatgcgcca ataaggctgt cgatgtcatt gccgtccttt acgaatacat
     1681 aatcaagccc catggcggtg aacaggtttg tatcggcttt gccgttgctt tcacgcagag
     1741 cctttaaatt cttgtacata ccgccgtgat tttcagcaat agacatatcg ttatcgttta
     1801 ttataacgat aagatttccg tcaaattcac ccgcatagtc aagcgcctcc agtgcttcgc
     1861 cgccgctgag cgagccgtcg ccgatcaccg caatgacatt tcctgtttcg tgcttcagat
     1921 cccttgcctt tgcaagaccg agagcaaggc ttattgaggt tgatgtatga ccgatggtaa
     1981 agaagtcgtg ttcgctttca tagggatttg agtagccgga gatatcgtca aaatgctctt
     2041 cgtaaagaaa tccgtccttt cttcctgtaa gcatcttgtg gcaataagtc tggtgagaaa
     2101 cgtcaaacac cattttatct gtgggtgaat tgaaaacata gtgcagtgct atagtagcct
     2161 ctgcaaaacc aaggttaggt ccgcaatgac cgccgtgacg gctgagcttg aatataagag
     2221 cgtcacgcat ttcctgtgca agttttttca tttcttccgt gctgagtttc ttaacgtcag
     2281 caggtgagtt gattttttcg agatacattt cttttctcct tttcccgata attgtagatc
     2341 gccgagcgcc cgtgacggtg cgtatagatg cgcagagcgc cgatattttc tttgctacgg
     2401 gagtattgta tcatatggag tgaactccaa gtcaatagtt tttttgaaaa aaactaataa
     2461 aatgttgaaa atgggaattt atcatcgaaa ttgttaccaa attgtaacct ttttctaatt
     2521 gatttttcag ttgttcagtg atatacttat tatatttaaa agccaaaatg caataacact
     2581 tgaatggatt gaacatcatg ttaaaaactc cggctaaacc tttaatcgaa gaaaaaaatg
     2641 atatttacaa tgacggatat aaacaaaagc aaaagataaa aaggcataat cggctcagat
     2701 ctttcggcat tatgttcttg tcggttgtta tatcactttg tacgggaatt tctctttact
     2761 gcggatatgc gcttatagaa ggcgggcaac
//
LOCUS       DAWAOG010000264         2778 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4694_length_2778_cov_2.100992, whole genome shotgun sequence.
ACCESSION   DAWAOG010000264 DAWAOG010000000
VERSION     DAWAOG010000264.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4694_length_2778_cov_2.100992"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    599..608
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          737 a          702 c          566 g          763 t
ORIGIN      
        1 gtatagctgt ttgtatctat atcaaaatat gctgtcggaa gctcgggata gccctctttt
       61 tctccaacca gctttttata ctcggagatc atctttgcat atgagcgtga tgcgaactta
      121 acgtctttca gttcaactgt ctgaccgttt cgctcaacaa caaaatcata gctgtaatac
      181 tgtgagccga tacctctcga ctgggagtta gacagctgga acgatatatc cattgttgta
      241 tagatattca tgccgttgat cttcaatatc ttatcgtccg cctgaagcac ggaagagctt
      301 atcgcaccct cctgaaatcc gcctatcgtg gtggtcgtaa cactgcttga acatagcacg
      361 gaaataacgc agaagataaa tccgagtacg aaattcataa aagcgcctgc ggctattacg
      421 agtatcctca tccacacagg tctgtttctg aagcttctcg gatcatcgtt ttcaacgtcc
      481 tcgtcaagca ttacagagcc gcctatcggc agtgccttga aagcaaacac tgtttcgccg
      541 atgcgttttt taaacagctt cggacccata cctatggcaa attccttgac cttcatatnn
      601 nnnnnnnnta tggcagagtt tcgctaccgt gaaatgtccg aattcgtgta caagtataat
      661 tataaagaac agaagtattc cgagaaaaat gttaagtatg ttcaaaagag cctccttatt
      721 ttaagcgcgg gcaattacgt attcccttgc cgcattctca ctgtcaagta tattaccgag
      781 tgtcacctca tcatgcacac ctacattttc gcaggcatcg gcaacaagct cgcctatgcg
      841 ataaaacggt atcttatcct caaggaacaa tccgacagcc gcctcatttg cactgttgag
      901 taccgtgcag gctgtgccgc ccttggcgat catcttcttt gcgtatttaa gacagaggaa
      961 cgtttcctcg tctgcctttt caaacgtcat agtaccgaca tcaaagagcg aaagccgctt
     1021 tgcgggtgac gggagtcttt tcgggtacgt cagcgcaaat tgtataggta tcctcatatc
     1081 cggtacgccc atctgaccta tgaccgagcc gtcctcaaac tctaccgcag agtgaagaat
     1141 actctgcctg tgaacattta tttcaacctg ctcgggctca actccgaaca gccacacagc
     1201 ttctataagc tcaagccctt tattcatcag cgttgcgctg tctgtagtta tcttctgacc
     1261 catatcccag ttagggtgct tgagcgcctg cgccttggta actgttctga gcttatcata
     1321 gctgtagccg aagaaaggtc cacccgatgc agtcagcagt atacgctcta tcttactgcc
     1381 gccgcttgcc atcatcgact ggaatatagc cgaatgctcg ctgtctatag gcagtatcgg
     1441 tagttttttc tcttttgcct tacctataac aagctcgccg cctgttacca gtgtttcctt
     1501 gttagcgagc gctaccctgt tacccgcatc aagcgccgca agtgtcggac gcagtccaac
     1561 cattccgact accgaattta cgaccgcatc ggttttcatc gatgccactt cgcacagtcc
     1621 gtccattttg cacagcactt ttatatccgt atcggcaaga cgctttttca tatcgctata
     1681 acaattctcg tcatatatgc agacaatatg cggcataaac tcacgcgcct gcttttcaag
     1741 aagcccggta ttcttagcgg cacacagagc tgtcggtttt atgccgtgca tacggcacac
     1801 atcaagtgtc tgtgtgccta tcgaacctgt acttccgagc agtgctatat tctttataat
     1861 actcaattgt tacctcttta tgacaaataa tgccgaagag tattccgctt cggcattgta
     1921 gttaaattta cgattatgca attggtgtca cgggaaaata tatctggaac atcatatatg
     1981 caaagggtgc ggcaagcaga acactgtcaa agcgatcaag aataccaccg tgtccgggca
     2041 gtatattacc gaaatccttt acggagcatt ctcgctttac aagtgatgcg gacagatccc
     2101 cgactactcc gagtcccgat atgatcagtg ccagtacagt gagatatacc cagtttacgc
     2161 tgaatgtatg cacaccaccg tttatcatgc tgtcgataag ctcatatgca aagctcatta
     2221 taacagcaaa aacacccgat gtgattattc cgccggcaaa gccttcccat gtcttcttcg
     2281 ggcttatcgc agggcacatc ttatgcttcc cgaggaaagt tccgacaaaa tacgcacccg
     2341 catcgccgat ccacgcgctt accatagtgt aaacgataag gaatgtggcg tgctcaggga
     2401 attttgagcg cataaacgct atagaacaaa gcgcaagagg tatcgctatc gatacaaatg
     2461 caacaagcgc cacctgctca aatcttgcct tttcgtgatt gaaaagcatt ccgagcagca
     2521 ttataacgac aaatccgaag tatatcagct ttacattgtt tctcagcggt tctatccaga
     2581 aaatgatagg tgttatcgcc gcaaagataa ggcttactgc agacgaaaac ttgttcttga
     2641 gatattttgt cgtaacgagc atttcataaa cggctataac cgaaaatgta gtcatagcaa
     2701 cataatagag tacctcgttg ttaagtgcga taattaaaat accgatcggt atcgctacca
     2761 aagcggtcac tattctgg
//
LOCUS       DAWAOG010000265         2775 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4704_length_2775_cov_1.719485, whole genome shotgun sequence.
ACCESSION   DAWAOG010000265 DAWAOG010000000
VERSION     DAWAOG010000265.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4704_length_2775_cov_1.719485"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          646 a          665 c          600 g          864 t
ORIGIN      
        1 gtcaacgata agaccgaccg aaacgtcgcc tgtcgaaacg attattatac aggtacggtc
       61 gttaaaaggt atctcttctt tgccgaatct gcttctgata tttacgaccg gaactatctt
      121 gcttctgaca ttgattatgc cttttatgta agacggtaca ccgggtacag ccgttatcgg
      181 cggtacttct attatttcta tgacatcagg tatctcaatg ccgtatacct gatcgcctat
      241 atagaaggtc agcaccttcg taatcactgt gttctgagga tctttctttg cgtcctccat
      301 ggatttctga gcgttcagaa gctcctgcag tttatcatta taattttctg acaaagtctg
      361 tccctccgtt tccttagccg ataagattat tgacatcgat tatgagcgat atcgtgccgt
      421 cgcccattac cgtacagcct gaaagacctt cagcttttat attgtactgg ctgagatact
      481 tcgggaacgg ttttactact accggctgtt ctccgataag tttgtctgca aacaggcata
      541 cattctttcc gccgacatcg acaagaagca ggataccgtc ctcaagcttt gtgacctctg
      601 taggcatatt gaacttttcg tgcagacgga ggataggata gcacacgcct cttatcatta
      661 tcatttcgcc ctgctttgta tcgttgagca gctggttgtt tgcaaccata aggctttcct
      721 tgatagatga gatcggcact gtaaagatag tagaaccgac tttcagtttc atgccttcta
      781 ctattgcaag cgtaagcggt attcttataa taaagcttga gcccttgccc tcttcactct
      841 cgaccgtgag tgtaccgccg cactgttcga tattactgcg tacaacatcc atacctacgc
      901 ctctgccgct gtattccgta accgtatcat tggtcgagaa gccgggcagg agaatcatgt
      961 tctggatttc cttcgttgta tattcgcttt cgggctttgt aagaagtccg ctttcctttg
     1021 ccttttcaag aaccttttcg cagttgatgc ctgcaccgtc atctgaaacg gttatgataa
     1081 cttcacctgc ggagtgcttt gccgaaagct ttacagtgcc cttttcaggc ttgccctctg
     1141 caatacgcac atttatatcg tcctcaatgc cgtgatccat acagttcctt acaaggtgca
     1201 taagaggatc gttaagattg tcaactatgc tcttgtcaac ctctgttgtt tcaccctcaa
     1261 gcacaagctc agcttcttta ttgagcttta tcttcatatc gcggacaatt ctgcccatct
     1321 tctggaatac gcctgcaagc ggtaccatcc tgatagacat aaccgtatcc tgaagctcat
     1381 ctgtaagctt tctgagctgt cttgcagcct ttgaaaaact ttcaagctca agaccctcaa
     1441 gatcggggtt tgcgataacc atagattctg tggttattat ttcgccgacg atatcatgaa
     1501 gcgtgtcaag cttcgagaga tttacactga taaggctctg tttttgtccg ccgtttgcct
     1561 ttttgatatc ctcctgtgcc tttttgaccg cgctctttgc ggctggcttg gcaggagcgg
     1621 gcttttcggg agcttttgct tcttctgcct tagccgcagg agctggtgca ggtgcaggag
     1681 caggagcagg agcaggagct tcaggctgag ccgccgcggg tgcgggtgca ggctcaggct
     1741 ctgccgcagg tgcggcggga ttatctataa cctcgtatga ttcaacattt acagcacctt
     1801 cgatagtctt tagtactgag tcattgtttc cgtcaaagcc cttgaatgtt acaaggaagc
     1861 cgttctcaac gatatccttt gccgtatcgg cgttcgattc gacatcggga ggagtacagc
     1921 ttatcacttc cgcttcttcc tttatctttg tcagaagcag gaatgcgcgc agattttcca
     1981 tcttgcaacc gtcttcaaag aatacacgaa ctgtcatact tcgggcatgg ggcagagaat
     2041 ttcttcaccg tcggcagaag cggcaggagc ggctgtttct gtggcgggca cagtgccgtt
     2101 ttccatagcc ttaagctttt ctgttgcatc ttcaagcttc ttgaatgttt ctgagaagtc
     2161 tgtcggagta tagtcgtcat cgcccgagtt ctgtatatat tcgatctcgg ccttgtaaag
     2221 gtcggatact tcgaatatga gttcatatac aaaatgagcg ttttttgcaa tttcgggctt
     2281 ttcccttatt acaaagaaca tatcctcgcc cttgtgtgca aggtgctgga gattttcaaa
     2341 tcccatcatc gcagatgagc ctttgatcgt gtgcatgatt ctgaatatct cgttgatatc
     2401 gtcctgagag aatacattag cgtcctctgt cctgaggaga atttcatcga gctgttcaag
     2461 caacgtattg gtttcaaata tgtacatttc gagcatggat tccatgccgg gttcaacctt
     2521 tgccatatac aaaaccgcct ttccctgatt atttttattt tgcaatcgta agattgatat
     2581 tgataaatcc aaaaaaatgt gctaaactta ttgtcaagca gtgtgatgca gtaatttaac
     2641 agtgcatctg aaaggaaata tatatgccca acattccaca gattacagct cccgtttcca
     2701 ataacaagag ctattctttt accaaccgac aatatgacgg caacattgaa ccgtttgata
     2761 tggtcgagct tgctc
//
LOCUS       DAWAOG010000266         2764 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4724_length_2764_cov_2.095238, whole genome shotgun sequence.
ACCESSION   DAWAOG010000266 DAWAOG010000000
VERSION     DAWAOG010000266.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4724_length_2764_cov_2.095238"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          809 a          605 c          718 g          632 t
ORIGIN      
        1 gccgtaggca gggcggcaaa gctgtcaagc acagtaagcc ttaccgcttc gactgccgca
       61 tcctcacttt tcattgccat acgggctatt ttatatatag gtcggtagac agccttataa
      121 agctgtgcat aaccgtctgt gtcacctgtc tgtgcctgct ttatcaaagc ggcgagttcc
      181 tgtgcggtca tctgttcacc tcaaaatgta catactcaca ataagaaaag ccttttcccg
      241 aaattttcac attacgcctt attgtatgct gtactttgta ctgtttatat tgcagtctga
      301 cgacagatat ggtaaaatac aagacaaacg gaggaatgca tatgaccgaa aacgaattta
      361 tacagcgata tacttcacat ctgagcgctc agcaaaagca ggcggttact gctaccgagg
      421 gggctgtact tttgcttgcc gtgcccggaa gcggaaagac gaccgtgctg ataacacggc
      481 tcggctatat gatatactgc cgcggcatag caccgtcaga gatacttgcc gttacctata
      541 ccagagccgc gacatttgag cttaagaagc gtttctccgc gtttttcggg agcgagtacg
      601 caggaagcct tgagatacgc acgataaacg gcttgtcctc aaagatcata gaacggtacg
      661 gaagtgtgca cggtgccgag agcattccga agcttatgag ccgtgagggc gaccgcaagc
      721 ggctgatatc ggatatatac aaggatatca ccggagatgt cggcgcgatg atcacatatg
      781 caaagaatat gatgctgaca gacgagcaga tatcggagct tgactgcggc gttgcggata
      841 cactaaagat ttataaaaag tattgcagtg agcttgctgt cagagggctt atggattatg
      901 acgaccagat ggtgacagcg ttcgatatac ttacaaagtg ccccgatata ctggataatt
      961 atcacggaca ttttcactat atatgtgttg atgaagcgca ggatacatca aagatccaac
     1021 acgagataat aagagtgctc gcaaagggca gcggcaacat atttatggtc ggcgatgagg
     1081 atcagagtat atatgcgttc agaggggctt atcctgatgc gctgaccgac tttgaaaaga
     1141 cctatgagaa cgcctcggta ctgtttatgg agcggaattt ccgctcaacg cccgagataa
     1201 tcggtgcggc aaacgcgttt gtcagccgca accgcttccg ccgtgacaag actatctgcg
     1261 ctgtgaacga agcgggaatg ccggtcagaa cggtacattg ccgcggcaga gctacacagt
     1321 atgacttcct gtgcggcatc gcaaaaagaa atgaaatgca gacagcggca ctgttccgca
     1381 acaacgacag cgcggtggcg cttgtcgata tgctggaacg aagcggtata ggctacagac
     1441 ttaagggcgg cgaaaagaca ttctttacaa gtaagcccgt aacggatatc gtatcgatca
     1501 taaactttat aaatgatcca tataacgttg atgagtttat gcagatatac tataaaatcg
     1561 gtctgtatat aaacaagcag gcggcacaga cagcctgccg tataagcgcg gcaaggcata
     1621 tacctataac cgatgcactg cttggtccgg gtgaacccac ggtcggcggc ggaatagagc
     1681 tgacggaaag ctcgcgtaag aatatcatgc gtatggcgga atatgccgct caggcgcgca
     1741 gtgcgggagc atcggaaaca ctcagcatca tatggcgcac aatgcagtac agcgactatg
     1801 ttcacaagaa taatcttgac ggaggaaagt acgacatact gcgcttactc gcaagaaatg
     1861 tgagcaacgg tgagcaattg acagcacgac tcggtgagct gagtgagata atggcaacgc
     1921 ataccgatga ccccgacagc cttttcacga tgtcaacgat acattcaagc aagggtcttg
     1981 aatacgaccg tgtttatctg cttgatgtga tcgataatgt actgccgtca attacatcgg
     2041 acaagcttga tgataaggaa gatgtaaagc agtatgagga agaacgcaga ctgttctacg
     2101 tcgctgtgac aagagccaaa aaggagctgt atcttttttc atgcacaggt gaaagctcgg
     2161 aatttgtaag cgaggtcgac cgtgatatcc cgatcgagta caccgacagc aatgacattt
     2221 tcttttcgct gtttgcaagg gatatgctcg ggcgcgaata ctgcgaccgc gaaaacggca
     2281 agggcgtggt tacggcttac tgcaacgata agctgtatat aaggtatgca agcggcaaga
     2341 gcgagcttaa gacgcttgaa gaaatgctga aagaccgcga caggaccgca cactatatta
     2401 ctaaggaaga gcttgacaag ctgaacacca atgacagcgc aaaagcagat atagtgccgt
     2461 caaagcggat agttatgccc aaagagggag ataagttgta tcacggcgtt ttcggtaacg
     2521 gcgttatcag aagtatagac aagctcggca taggcacggt gtactttgaa gcaagcgggc
     2581 agacaaagcg ccttatgctt gaaacgtgtg tgaaaaacgg gatcattacg gtgtagtaat
     2641 tgtttcgtag atgcgcagat ttatccggag ataaatatta tgtctgctca ttttcgctaa
     2701 ggcagaatga tgaaacatac agccaacacc gctgaaaaca tatccatacc gaaaaggtgt
     2761 taat
//
LOCUS       DAWAOG010000267         2743 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4775_length_2743_cov_1.958333, whole genome shotgun sequence.
ACCESSION   DAWAOG010000267 DAWAOG010000000
VERSION     DAWAOG010000267.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4775_length_2743_cov_1.958333"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2051..2060
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          572 a          824 c          545 g          792 t
ORIGIN      
        1 ctatgctgac tgcatttaca gccgccgtga tatccgccgc agtcgccgca ccgacctcct
       61 cggcggtata tgacggcttt tcatcggctt tcgcccagtc cgctatatca gtatttttaa
      121 gatacggtga caaatcaacc gaaatacccc cgtcagccgt taccgatata ttatccccta
      181 ccattacacc gccgagcgta tcggctgttg ccacgggcaa tgtatatcca acgccgtcaa
      241 cgccgccgtc cgcgataaac ctgccggata tatgcagatt tcccgcgcgg tcaagcacca
      301 gcgcgtcact tctgaactca ttttcaccgc ttccccaacc gttgcccaca acgagccaca
      361 catcttccgc cgtcttgtta tagcttccaa gcgcaaaaac ttcccagccc gcagccgtgt
      421 gatttcccga tgcgtgcgag tgctcgccat acgctctggt ataataccct tccgcgtgtg
      481 agttactgcc gcttgccacg cacccctcgt tctccgcgtg agatgacgat cccgttgcag
      541 aagtgccata cccttcggcg tgtgcctgag tatttgccgc tgtagtttct atgccctccg
      601 catggctgta cggagcgtta gccgtagtct tatacccctc cgcatgagca taatacgagc
      661 ttgcgacatt attctcatag tcgttgaaaa tctcacagtt cttatcggcg tttgtgaact
      721 cgcccactcc gtccgacaac gcctgatata tgagcttttt ctcctcttca ccgtccgact
      781 ttgcggctac atcgtcattt atatccgcaa ggcagaactt tatcctgtcc gcaagcagcg
      841 agatgaagtt ctcgatatca ttcagccgct tttcgctatc tgccgccttg tctgcggcaa
      901 agcttatatt gatattatcc acttatctca cttccctttt cactgtcaag atatactccg
      961 taaagtaccg cataagcgtc cccgcttatt ttgccgctga tttttattct tacggtctta
     1021 catctgagcg gtatgatcgg cgcagctgtt atttcttcgc tttcgctttc ggtgctgtta
     1081 tattccgccg cctttttcca tcctccgccg tcaccgctga catacagtga aagctctgga
     1141 acttcattat tcttgcggct gtaccgcaca gcggctctga gctttttgta tatattgaac
     1201 acctttcctc tgccaatatc tccgctcacc gcttcaaagc tgttttcagc ctgctttttt
     1261 gcatcgctgt ccgttccgac aagtcttatg accgtcagct ttccgctgtt tccctttgcc
     1321 accgcataca gtctgccgtt tatctcatga gccgaacaca caccgctgag ccgtctttcg
     1381 taccatgccg cgtatcgttt gtcatagaag tacaccgtcc cgtcagctgc cgccataacg
     1441 atatatcttc cgtcagccgt catgaccgcg gtatctgtta tatctccgcc gagctttgaa
     1501 tctatcctgt gcgccgcaga accgttgaag cagaacaccc cctcgggtgc tttataatac
     1561 agcaatccgt ccgaaatgca cagtgaaccg ccgctgccgc tctgcacacc cctcagcttt
     1621 atcgtactca ccgtaaagtt tgacgctttt gtaccgtaaa cgatatgcac acaattctcc
     1681 ttgaaaaaca gcacactgtt cgcgtatacg caagcccccg taaagtcgcc gtccgagcct
     1741 acggtagccg cccaggcatc ggtcgatatc ccgtcatagc tgccccattc cacagcgctt
     1801 ccgagtttgg agcagtatat ctcgtgatca gcggaagagc acccccacag tctgttgttg
     1861 tgctcaacaa taaaaaccat ctgagggatc ttcatgctta cagtcgcatt ctttatgcac
     1921 actatcctga agttttccat tcttctgccc tccgtataaa agtaaagcgt gtcattatat
     1981 acggaggtat caagatttat agatacacag ccgctgctcg tatagctttc ttccgatatc
     2041 gattttatcc nnnnnnnnnn aggtattttg ttttgccgcc gtagctgtac gctatcttta
     2101 tctgattgcc ctcgctgtaa taagaaagct ccgcactgtc ttttgcaaca tctatataca
     2161 gtcgcttgaa gatcgccact gtcgggcgcg tctggttctg attatactca tacagcgagc
     2221 ctttcagctc atgccttgtc ggatatgtca cacttatcgg atcatcggcg gtgtttataa
     2281 gcgcctcgtc cggcaatata agtatatatg cccccatacc gacaagccgc ttttcgcctg
     2341 tactaagctc aactgcggtt ttctttccgt caatatagat gccgtccgcc tttgtgtaga
     2401 ctatcccgcc gtttttgaac atatatccgg ttattccgtc aggaagctcc gtatacgaaa
     2461 acggcttaca ggtcctcagc gcgggatacg cggtaaagtc cagcccttta agttcctgcc
     2521 agtatccgag cggagttccg ttagaacggt ctattccgcc gaacacttcg gcgctttccg
     2581 taacgttctc gattttagac actttcgccg ccattttttc ctatcctttc atccttgttt
     2641 tcacattact catatgcctt gcgttccagt accgacaaaa ctcatcatac agcgtttcgt
     2701 aaagtgcctc gtcattggca tatctctcat attccgcatg ata
//
LOCUS       DAWAOG010000268         2738 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4785_length_2738_cov_2.143496, whole genome shotgun sequence.
ACCESSION   DAWAOG010000268 DAWAOG010000000
VERSION     DAWAOG010000268.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4785_length_2738_cov_2.143496"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    514..523
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          805 a          502 c          776 g          645 t
ORIGIN      
        1 ctgatgtgac agtaagcatt gacgctcctt ccgataagct gaaaagcggt gacagcggtt
       61 atgctacgat agaaacaggt ataaagcgtg atatagtaac tctgccttac agcgcggtga
      121 tgcaggacgg caatagcgaa tatgtatatg tcattgagga cggcagagcg gtaagacgtg
      181 acgttgtgac cggtcttgag atggcaaagg tgactcaggt gttcggggtg agcgttgagg
      241 aagaggtcgt taaggataca gcaggaatag cggcaaacgc agtgattatg cgaaaccgga
      301 aagacagctc cgacagcggg aatatatcgg cagaaaacgg taaaactatg acggactaaa
      361 aagagcggag gcagtggtat gggttttgca tcggtaatgg ctgatatatg gagaagcaaa
      421 agcaggagcg cgcttacaat gagtgcggta gcaataggta tcttttctgt gacggtgata
      481 tcggcggtcg gacagatagg tactgccagg atannnnnnn nnngggcgtg aactcggtgc
      541 tcgtggaggc acaggaggcg ggagtaaccc tcggagataa cgatattaaa aagctcggag
      601 atttgagcgg agtgacaggc gctatgccgc ttatggcaca ggtcaccaag tgtgagctta
      661 tcgaaagcaa tgtttcgtgt atggtgtggg gcgtaaacga aaaagccgat gagataatat
      721 ctatgaagcc gatacacggc aggcttatca atgaagatga cgtggaaaaa gcggagaaca
      781 tctgcgttat cgatgataag atagcactcg aaacatacgg caggacgaac atagtcggaa
      841 agacggtgag cgtgaatttc ggcggcaggt ataacgattt tacggttatc ggtgttgcag
      901 agtcggggct cagtcccata cagaatatga tgaatgacgc tgtgccgtac tttgtgtata
      961 ttccttacac gaccgctcag aacatctgcg gcaagacaga ctatgacaag atagcggtat
     1021 tgcttgacaa gagcggtgac ggagaaaagg cgatagacgg aataaaagcc tgtatggctg
     1081 atgtaaaggg cgagggcaat ataaagatca gcgagcttca aagccagaag aaacagcttg
     1141 acggaatact gactaccgcg acctctgcat tgtcgttcac ggcgggaata tcgcttgcgg
     1201 tagcgtccgt gtcggttatg accgcaatgc ttgtaagcgt cggtgagcgc aagcgtgaga
     1261 taggcataaa aaagtccctc ggtgcaagaa atataagaat agtaggcgag tttttagccg
     1321 aaagcacgat gatatgcgtt atcggcagta tagtcggcat tgcggcaggg tgcgcggtgg
     1381 cgtttgtcat aggtcttgcg gtcggtgaga gctttgttat gcagacggat ataatgctta
     1441 tcgcagtcgg agtgtctgcg gtcataggta tgatttcggg aagctatcct gcttataaag
     1501 cggcaaggat gaagccggtg gacgcgctta agatgtaaaa atttaaaaaa ttttttcact
     1561 accctcttga cagctaacga ttattagctt ataatatagc taacgatcgt tagcttattt
     1621 acgtcgaggt gaatatatga ataaatcccc aaaaaaggaa gacacaaaac aacgcatact
     1681 tgacgaagcg ttgcggctct tttcgcagag cggttatgac gcggtgagcg ttgaacgcat
     1741 cgcaacggcg gtggggataa aagcgccttc actgtacaag cactttaaaa gcaagcagga
     1801 gatatttgag gctatcgttg cggaaaccta ccgccgttat gatacattca cggacggcat
     1861 tgatgtgcat gtttccgaaa gcaaagccga tgaaaaggtg tttgacggta tttccgagga
     1921 aatgcttgcc gaaaaggtaa aacagctgat agaatactca ctgcatgatg agtatgtaag
     1981 ccgattcagg cgaatgatga cgatagagca gttcaggaat gccgagtttg cggcgatgta
     2041 ttccgagcgg tatctggaaa gattggtgcg ttatcacgca ggacttttca gagcgcttat
     2101 caagtcggga acgataatcg gagaagaccc cgatacgctg gcgcttgagt atgttgcgcc
     2161 tgttgtgctt atggttgaaa tttgcgacag acagcccgaa agggaagcgg agtgcatgaa
     2221 tcggcttgaa gcgcacgtca ggagctttta tcgtacatat tcgccgcata tggtgaaaaa
     2281 ggcagagaaa agaggcggca ggaatgactg aaagccgttt tatactgtgt cctcactgca
     2341 aagcaaaaac aagggtgaag ataacaaagg acacggtgct taaacagttt ccgctttatt
     2401 gcccgaaatg cagaaatgag agcgtaataa acgcggaaaa ctacataatt acgttaattc
     2461 agacagacgc taagacgcag tgctgaccta tttaaaggca gcactgcgct atttttttga
     2521 aaagaggttt tttatatgaa attatatttc agggacggag ttacaattgc taccactatt
     2581 atgcttttaa tgcttgcagg gtacataatt ttctgcatag caacgcgcaa gtcgaccagg
     2641 cactgggggc tgagaagcct tatattgctt atattcgggc ttgtttgctt atgtgtgata
     2701 aagaagtaat agaagtgtaa aaaattttgc ctttccta
//
LOCUS       DAWAOG010000269         2737 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4786_length_2737_cov_1.843400, whole genome shotgun sequence.
ACCESSION   DAWAOG010000269 DAWAOG010000000
VERSION     DAWAOG010000269.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4786_length_2737_cov_1.843400"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          719 a          612 c          511 g          895 t
ORIGIN      
        1 cccgattcgt agtatcactc agaaaatcat gcatttctac tgtcgtaatt ctcatcggca
       61 aaatcaagcg ctgcaagcat ggcgatatct gtcagtgaca ttgaagatgc cattgagctt
      121 aacttatcta tttttttctg cagattttcc gcagcatcat acattctgtc gggatcttcg
      181 ctcttcaagt aaaaatcgtg tccgcttata gatattttta ctttttccat tataccctcc
      241 gggatttatt tataaaactt attttttgtt atcaatattg cgacaatgac ggcaagtata
      301 acggaaatga caaccggaaa ccatgtaaac ggaagcggca gatctaccac attcatacca
      361 taaaagctga acaccattgt cggcacagac attacaatag taatgaccgt cagtaccttc
      421 attacgatat tcaggttgtt tgatatgaca cttgcaaatg cgtccattgt acccgaaagt
      481 atgtttgaat agatattgct catctcgata gcctgcttta cttcgatgag tacgtcttca
      541 agcagctcct gatcttcttc gtagagtttt attactcttc ctctcaatat cttttcaagc
      601 actgtttcgg tagatttaag cgacgttgag aaatatacca gagatttctc aagtccaagg
      661 agctgtataa gctctttgtt tttcattgac ttgtgaagtt gctgttcaac atagtttgaa
      721 agcttgtcta tctgctttaa gtataacaga tatttacttg ctatcctgag cagcatatgg
      781 aatacaaagc gcgtcttgaa ctgtgtctgt atccctttta ctatgccttt tgtaaactca
      841 tcgatgatta tgttttcctt aaggcagaca gttataacgt ggctgtcggt tattatgata
      901 cccataggca tcgtgtaata actgatcgta tcgtccttat cctgcacttt ttccgctacc
      961 ggatagtcaa ggactataag tgtctgtccg tcatcgctct caatacgtga agtttcttct
     1021 tcatcaagcg cggaacggat aaagtcacgg tcgatgttca gttcttcctg aagtgaggat
     1081 atctcggttt cggtaggcgc tacagcagac acccagcaac cgggctccat tgtttcaagt
     1141 tcaactattg tgttctctac agtcttgtaa aatttcagca taacgtacct ctttcctgca
     1201 cagaaagccg ttatgcaacg actactgcgc acttttcgtt ctgttttaat cgtttcccgc
     1261 aagggtccgc agtcatagtc tttcacctct ttcaaagttt ttgcatagcc ttatatattt
     1321 tagcatactt tttacggttt gacaacagtt ttcagttcat tttgcgattt ttttcttccg
     1381 ttttttcttt ctctttacgt tcagcctgca tacggctgta aacgcaaccg caataattct
     1441 gacgatataa gccgtattcg gcggaaagtt caatagagcg cttgtatccg ttgcgctttt
     1501 tgaagtccga gcagagataa ggcacatcgt attctttgct gagtatttct ccgagctcat
     1561 tcagttttgc ggcattttta tgagggctga cagaaagcgt tgtacagaaa tattcaaact
     1621 taagttcttt tgcaagcctt gccgtttctc tgagtcgaat ttcgtagcac aaaaagcagc
     1681 gctcgccgcc ctcgggaata ttctcttttc ctttagccat agcaaaaaac aaatcgggag
     1741 aataatcgcc gtctatcatt tttacgggat atcggaaagg cttttccgat atcagccgct
     1801 taagctcatt cttgcgcttt tcatattcgt ctttttcggt tatgttagga ttataaaaaa
     1861 acaccgttat attgaaatgc tccgcaagat attccagcac atagctgctg cacggtgcac
     1921 aacacgcatg aagaaaaagt gttggagcgt ggtcaagcga ttttataact ctgtcggttt
     1981 caagctgata attagtcatc agccttcacc agcttttcat ctgctatctg gcggagctcg
     2041 tcaagcgtgc cttccttgat atcattttca tttatgataa aaagataccc gtcatgtgtt
     2101 acgatatatg cgcttgattt attgaaatca acggaagcta cagccgtcca gtcagcaata
     2161 gcctttgccg caccgccgtt tttaagtacg ataccgctag ctgttatctc aattgcatca
     2221 taaccgtttt ttgaattgat atcgaaaatt tcttttgtgt atttttttag cgtcgccgaa
     2281 agtataagca ctgtcgcaac tatgagcagt ccgcagatca cggttgcaaa tatgaaccac
     2341 cacagctgtg tgataacagc aagcacaacg ccgacagccg ctataagtcc cattgcgaca
     2401 aggtagcaga tcacatatac ttttgaagct ttattcttgc cgaaaatgtt gtactttaca
     2461 aactcgctga cattttctga ggtatatttc aattctgagt ctatcatctt atactccctt
     2521 catagtaaga gaaatgcgtt ttttgccagg gtcgatatca agaatcttga cctttatgat
     2581 atctccgaca tttacgactt caagcggatg ctttatacgc ttatcgcata tctgcgagat
     2641 atggacaagt ccgtcctgat gtacaccgat atctacaaac acaccgaaat ctattacatt
     2701 tctcaccgta ccgtcaagca ccatgcccgg ctttaag
//
LOCUS       DAWAOG010000270         2730 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4807_length_2730_cov_2.099813, whole genome shotgun sequence.
ACCESSION   DAWAOG010000270 DAWAOG010000000
VERSION     DAWAOG010000270.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4807_length_2730_cov_2.099813"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          694 a          712 c          527 g          797 t
ORIGIN      
        1 gagctgttct atgataggaa cctgtacgtt ataagagcgt gctatatcgt atgcgatctt
       61 tgcacattga taaccctcta ccgtgccgac ctgctttaca gcttctgcgg caggtatacc
      121 ctgaccgata agaagccctg cacgatgatt tcttgaatga ggagaagtac aggtaactat
      181 cagatcgccg acacctgaca aaccgtcaaa cgtttctctt ctgccgccca tggcaacgcc
      241 aagacgggtt atttcggcaa gacctcttgt cataagcgca gcaagcgtat tgtcaccgag
      301 ccccattcct tctactattc cacagcagag ggctatgggg ttttttaacg ctcccccgag
      361 ctcgcagcct ataaggtcac tgtttctgta aattctgaag cgatcgttct gcaaggtttc
      421 ctgaagatac agcatacttt cttcatcttc agatgaaaca acgatcgttg tcggcatatt
      481 tctgcctatc tcttctgcgt gtgaaggacc ggtaagtacc gctatacggt tgtccggaag
      541 ctcctctttg agaacctgcg acagacgttt atctgtaccc tcttcaaagc cttttccgac
      601 attaactact atagtatcct ttttaacaaa cggttttgct ttctgtacaa cctcacgcac
      661 aaatttagaa ggtatcgcaa acaccaatat atcgcagtca cccgcacagg aaagatcggt
      721 agtgaaatat atcgtcggag gtactattac accgggcaac agctttttat gctcaccgtc
      781 attgagtata gcatctattt cttccttgta ctttgaccat gctgttactt tgtgaccgta
      841 tttgttccac attattgaaa gtgccgttcc gaaaccgcaa ccgagtatag tgatcttcgc
      901 cattgtaaag ccttctttct tattatgctt tctgcccaag ttttttttcc gtatgatttt
      961 taagtcttat gagatttcct ctatgcataa acagtatcag cacacaaata agtgcagata
     1021 gcaccacatt tatccatatg caagtatcac cgactatcat actgtatatc aatatcgtta
     1081 ccggataaag gatcgccgca atgcacgagc caagcgaaac ataccttgta atagcaacca
     1141 ctatgataaa tatcccgagc agtatgcagg caggtatcca ctctaccgtc ataagcaccg
     1201 caacggaaac gagtataccc ttgccgccct taaatccgaa atataccggg aatacatgac
     1261 cgattatagc cgcaactgct gcggcaaatc ctacatatgt cattgagttt gccggatctg
     1321 cagtgtctat accggcaagc cacaaaaacg ccgatctgac tataagtata gacagcacac
     1381 ctttgagcac atcacccagc agtacaaata acgccgcaag ctttccctgt gttctcaacg
     1441 tattggtaag tcccgcattt ccgcttccga gtgtccttat atcttctcct gttttgatct
     1501 ttgtaacgat tatagcactg tttattccgc aaagcagata cggtactatg atcattatcg
     1561 catagcacag atatatcatt atccgttatt ctcctcgttc tcttgctatt atttttatag
     1621 gtgttccgtc aagcccgaag ctctctctta tctggttttc tatatatctc tgatatgagt
     1681 aatggaacaa ttctttatca ttacagaata ttacaaacgt cggcggttta gccgaagcct
     1741 gtgtcatata ataaagcttt aggcgcttgc ctttatcaga cggaggttga acccttgccg
     1801 ttgcatagcc gagcatatcg ttgagcgtac ctgtagaaat acgtcttata ttctgcgaat
     1861 atgtgtactt tatctgctca aacagcttgt caactctctg acctgtaagt gccgaaatga
     1921 acacgaaagg cgcatacgac ataaacgaga aatcgacctc gagcttcttt gtaaactctt
     1981 gcattgtctt accgtctttt tcaacagcat cccacttgtt tatcgcaata acgctcgcct
     2041 tgccctgttc gtgagcatag cccgctacct tgctgtcctg ttcggtaaag ccctctgtcg
     2101 catcgatcat aataacacag acatctgctc tgtcaactgc catataggca cgcagtacac
     2161 tgtacttttc tatctgctcg ttcacttttg acttgcggcg gattcctgcc gtgtcaataa
     2221 ggacatattc ctgcccgtca cgctcaatga ccgtatcgac tgcgtcacgg gtagtacccg
     2281 cgatattgga tactatcatt ctctgctcgc ctgcgatttt gtttacaagc gatgacttac
     2341 ccgcattcgg cttaccgatg accgcaacct tgatagcgtc ctcggaatct tctcgctgag
     2401 ggttttcttc gggcatatta tcgaacaccg catcaagcag atctcccgta ccgtgaccgt
     2461 gtaccgagga caccgctatg gggtcaccaa gaccaagatt atagaattca tagaaatccg
     2521 gcggtacttc acctattccg tcacacttgt ttacgcaaag cacaaccggc ttgccgctct
     2581 tctggagcat tgccgcaaca tctgcgtcat ttgccgtaac tcccgatgta acatcggtca
     2641 cgaatatgat aacgtccgcc ttgtcgatag caagctgtgc ctgcattctc atctgcgaaa
     2701 gtattatatc cttactgtca ggctctatac
//
LOCUS       DAWAOG010000271         2718 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4839_length_2718_cov_2.324822, whole genome shotgun sequence.
ACCESSION   DAWAOG010000271 DAWAOG010000000
VERSION     DAWAOG010000271.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4839_length_2718_cov_2.324822"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1424..1433
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2084..2093
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          653 a          677 c          552 g          816 t
ORIGIN      
        1 atcagtccgc ctatcgcatt gacaatatac aagtgccaca cctgcaaggc gttacacgca
       61 aacagcagta taaccgtaac ggtacacaac gccgctatac tgtcgcatat aagcatggta
      121 ttctttttgt cgaacctgtc gcacagcgca cctgcaaata tgctgagcaa tacatacggt
      181 gcatatgatg tgatcgtaag catagctgta tacagcgctg agccgctttc tccgtacagc
      241 cagagtacaa gcgcatagct tgtcatagca cttcccagtg cggaaagtga ttgagtgagc
      301 caaagcagta taaactgccc aaagctcttg ctttcattat ttttattcat aaccttgctc
      361 cttatatttt atgttttcaa aaaagcaagg tcagcggata atgtattaag ttttctccat
      421 gcggttatcc gctaagcctt gcatggagct atgatggcgc agattaccaa tgtaatcact
      481 ccttaagaaa atttaatttt aatcagaact tgacaatttc ctgcggcttt gtgaacgatg
      541 acagggtagc ggtggcattt ttaaagtaaa acacctgcgt attgccgtca tcagcatcat
      601 acagagcctt aagctcaggg taagcatcaa gcatagaaac cctggccgca cggtcgtcgt
      661 cctcgacaag ctccccttca attctcagcc agtcgccgtc tgccatagcg catatctcaa
      721 cattgggatt tgcggctatc tgatgcgata cagccttttt cttacctgtc tgtatataca
      781 gcttcccgtt gtagatgtga gccgtgccga aaggtcttac cctcggcttg tcaccgtcaa
      841 cagtcgcaag ataatatgta cctgcggctt tcagaaattt gcatacatca agtaaagttt
      901 tcatagatat acctcctgaa ttaaaaccct taacgggaat atagactatt ttataactgc
      961 cttgaaagcg ttgtaatttt gcgcttcgcg ctcggtatgg aataccgcaa attctatcgc
     1021 cttaaaataa cttccaaatg tctttaactg ctccgcaaac acctttgcta caacgcccgg
     1081 cgggttcata aacgcaccac agccgaaagc gccgagtatc agcgcttcgt tatcgtttgc
     1141 cgccgcaatt tcaaatatcc tgcgtatcct tgagcaaagt agctccgcaa gctcttcatc
     1201 ggtcactttc gcttgcttat cgcctgctcc cgggttcata ctgttgctcg gcttgggtcg
     1261 cagattggga gcggcacagg tgataatgtt caccttctgc cattcgcttt cgggtaacag
     1321 cttcggtaca cttgtatcgc tcttaataac atatacatcg ggcgtataga tacagtcgtc
     1381 attgtacagc ggatttcttg cggctctgtg cggagtgtaa aagnnnnnnn nnngtgtaaa
     1441 agcaacccca catatcacct gaattaagac aaggatatag tgttgaacaa cggcagatag
     1501 cctcctcctg agccgaagaa ccgtgagtaa caccgccgcc cgggttactt gccgaagcaa
     1561 agttcagtac acaggtcttc ataccttgct ttgcgtattc aacagccgct tcaagcgaac
     1621 gtttaccgct tactgtaact gtcggtgaat atcctttaga ctgcggcaaa tccaccgctt
     1681 cattcgctcc cgtaaatttt tggtttgcga tagaattgct cacagccgcc ttaagtacgg
     1741 cattgctctt atactgtttc attgtatcat caaaaacatc tatgttgatt tgtcgtttgt
     1801 cctgcatatt gttcctttcg tgtattattt ttctgacgct ccgcttagat cgttcataat
     1861 atcggaagca tcatccgctc cgtccatacc gtcaagcatt ttctcaacag tggttttatc
     1921 cttatcatcc tcatattcgc tcttgtattt cttcggcttg tcattatcaa gacggcgcat
     1981 aagtatcgcc tgcttgtata agtccggcat ctcctcggca agctttttcc tgtctgccat
     2041 aggcacctta tcgccgttca ttatccgcat ggctatctta aggnnnnnnn nnntaaggag
     2101 gttgccgtaa tcggtgaatt tcttaccgct tttttcagcc tgcttttctg cctcgcgcat
     2161 ttgtttgagc tgctgcattg cgctgtcata ttcctgcttt ttctgctccc ttagctgttc
     2221 aagccttgtc ttttcctcgg cgtgagcgtc ttcggaggta gtgctttcct gtgctttctg
     2281 cttctcatct gcacctgttt tattctgcgc agtctgccga ttgatttgat tttgcgcagg
     2341 cgagtacata cctgtcggtt taaaagttac gttcatatat attctccttt gtcatgacat
     2401 ttcatcgtat cgtaaataaa tcaaaagggc agacaactcc cgtcatctgc cccgatattc
     2461 agtttaatta ctttgaaaag ctgtacttat ccgaaaaatc aaaagtagcg aaataatctt
     2521 cgggcttttc tgcacggcgt ataagcttga acgagccgtc ctccttcaga aggacctcgg
     2581 cactgcggag cttaccgttg taattgtagc ccatcgagaa accgtgtgcg cctgtatcgt
     2641 ggatatagat tatatcgcct atatcgattt taggaagcat acggtcaacg gcgaacttat
     2701 cgcagttctc gcaaagac
//
LOCUS       DAWAOG010000272         2708 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4866_length_2708_cov_2.042216, whole genome shotgun sequence.
ACCESSION   DAWAOG010000272 DAWAOG010000000
VERSION     DAWAOG010000272.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4866_length_2708_cov_2.042216"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1075..1084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          778 a          654 c          571 g          695 t
ORIGIN      
        1 gtatgggtat atatatccta tgtaagaaaa cgtcttgccg ctatcggcgc agatattgaa
       61 atccgggcca agcgcagtgt tggctatact cttgcaaaga tcggggatac tgactgatgg
      121 taaaacagct acagaaaaag tttatcatat ccgctatgac agcggtaaca attctgcttg
      181 tcgtgcttat cggcggaata aacgttttca attatctcac aacaagcaac gataacgaca
      241 ggcttatgga gatgctctgc tacagctttg aaacctcaac aaagtggaac gcagatacaa
      301 ccgacaatac tcagcctccg cagagcataa acagcactca gcaaaataca accgacgcaa
      361 ccggcaatca gaatgatacc gggtttgtgc cgcaggacaa tggaactaaa ccgcccgacg
      421 ataaaaagaa caacggcttt ggcagacacg ataaaaacgc agtggattca gcgcgttatg
      481 ccgcagttgc gatcgacaaa aacggcaata tcataaggac ggatgtcact catatatcat
      541 cgcttaccga ggaggaaacg accgccatta ccgaagcact taaaaatacc gcttcgggaa
      601 caggcacata caacggtttt gtgtacagga taagcgaaac aaagcgtgca gacggcaagg
      661 tgatcattct gcttgacaac gctatgcaga taagctcgtt ctttacggta ctgtttatat
      721 ccgccggtgc aggaatattc ggttggcttc tgatgctttt gcttgttatt ctgctttcca
      781 aaaaaacgat cgcacctgtt gcgcgcagta tagaaaagca aaagcaattt gttacaaatg
      841 cgggtcacga gataaagacg ccgcttgcca ttatacttgc caataccgat gcgatggaac
      901 tgcacaacgg tgaaaacaaa tggagcagga atatccgtgc gcagacgctc cggctcagcg
      961 gacttatgca aaatcttctt atgcttgcga aaatggacga aagctcaaca aagctcccaa
     1021 tgtgcagttt tgatataagc acggcggctg aagatacggt aaatgcattt atagnnnnnn
     1081 nnnncaaggg cgtgatgata gagcaggata tccaaaaagg acttcagctc aatggcaacc
     1141 gcgacagcat agtacagctt ataacggtgc tcctcgacaa tgcagtgaag tacaccgaaa
     1201 gcggcggcac tattagcgca aagctcagtg gcaacgataa gaatatagca ctcagcatag
     1261 caaatacctg tgagcctatt gaccaccccg aaaagctgtt tgaccgcttc tatcgcggcg
     1321 acagcgccag aacgcagaaa aacggcggct acggtatagg cttatcggtg gctcaggcaa
     1381 tagcggagct tcataaaggt actatcacag cggaaaatat aaccggtaca tctcctgata
     1441 atcccgatat taacacggat aatatgctta tgtttacggt taaaatatga ataaaaaacg
     1501 gtttgcaaag cagctgaaat cagccgaatg caaaccgttt ttacatatat gaattttctc
     1561 attgcttttc gcggcgacac ttccccgcaa gatctatagc agccgcgata attatcacac
     1621 cacggaaacc tcgatcagga tatttccctt agcgtcctcg taccaggttt cccacgcaaa
     1681 atacttgtcc cagcaatcgc actcgctgaa atccatatat tccgcattga attccttcgc
     1741 ttcttccgcc ggcacaagcc agccgctgca atcgcttgca ataagcccgt tctttacctg
     1801 ctcgtgtccc tctttgctgt caagaaagaa cacgcagcct ttggtttcag ccgcctgctg
     1861 aactttttcc acaaatttct caaatccgag cggctctctc gttctgagtc ccgtcatcat
     1921 aatacacctt gcctttatat caaaatagtg taataattat accaccgctc tgcccccaag
     1981 tcaaccgtga tatacaaaga ctgagagccg cccactgtcc gtgagcggct ctttacggtt
     2041 ctcttacatc tggagcgatt ctattaccgc acctactgcc tttatcgcct tgccggtcgc
     2101 tttctttacg ttctgtgtct tgtttgaggt catagccttc gttgcggcaa aagcgatacc
     2161 gcctgctacc attgcagtgg cggcacatat catattattg gaagttttct tgctcatttt
     2221 ccattatcct ttcatatccg ccgttttcgg ctcgttaata tattgtgttt taatctcaga
     2281 aatattgcaa gataaatttt acaagggcag tttccaataa gaccgctttt tgcagtaaaa
     2341 atgccattca tacggcaaac gatccatata taaattttac atctcgacaa ttcggaaatt
     2401 ttatgttaaa atataaccga tgtgtaaaca gaaaggacga tttttaatgt cgcaacataa
     2461 aataatagcg gcgatccacg atctctcggg ttatggcaga tgttcgcttt cggttataat
     2521 accggttata tcagctatgg ggatacaggt ctgtcctgtg cctaccgcgg tcatgtctac
     2581 ccatacaggc ggctttaccg atatagcgat ggaggatctc acagaccata tttcctcctg
     2641 ctatcagcat tatgataagc ttggtctgaa atttgatgcc gtctacagcg gttttctttc
     2701 ctcggcaa
//
LOCUS       DAWAOG010000273         2700 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4885_length_2700_cov_2.134972, whole genome shotgun sequence.
ACCESSION   DAWAOG010000273 DAWAOG010000000
VERSION     DAWAOG010000273.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4885_length_2700_cov_2.134972"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    525..566
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1074..1083
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          710 a          698 c          510 g          730 t
ORIGIN      
        1 aaacaagctg gctcgcatcg gtcgctatcg ctgcaccctc aacgcccata ccaaagcctt
       61 gtatcagtat aaggtcaagc gctatattaa caaccgaagc cgctccgaga tataacagcg
      121 gtctttttga attacctgcc gcattgaata ttcccgcaca catattataa agcacgttga
      181 acagcaatcc gccgcagtag atctgcaaat agagcgatgc gccgtcaatt atattatccg
      241 gtgtctgcat ccaaacaagg aacagcctgc tcataaatac gcccgtgacc gtcagcacaa
      301 gaccgattaa aacagcgatc gccattgccg tatgtaccgc tctcttaaca tcatccttgc
      361 gcttcgcccc catatactgc gatatgataa cacctgcgcc gaccgatgca ccctggctga
      421 acgctatcag cagattgaca atagagcctg tcgcaccaac cgccgcaagc gcttccttac
      481 cgacgcagtt tcccacaatt atagagtcga ctgtgctgta caacnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnngcac tcaatttaaa cttcctttct tcatccataa
      601 atattatact catttaaaag aatatgtcaa cttccgttat cactgacgtt ttgtgccaca
      661 gcatattgaa ctgtgctaca gattgtgata aaatgtaaac aacaaaaaat acgccgaaag
      721 gacttgataa atatgacaga cgaaaagaaa aaagcacccg taactaccgc acagcccgac
      781 actgccgata aaagcgacta cagacaatcc gccgacaagg aagaggctat atgggatatg
      841 aaacaggagc ttgaagatta tattgaagat cagggtatct atatcagaca gtaattaaac
      901 tatcgacata aaccactcga ccgttgcggg gtcgtggtgc gagaagaaca ggctcttgcc
      961 tgtgtcgagc tttgctatag tctgcatatc ctctgccgac agttcaaagt caaacacttc
     1021 aaagttctgc tgtatacgct cgtgatgtac cgatttaggt ataacaacta tgcnnnnnnn
     1081 nnnctctgaa tgaggaaacg cagtgccgtc tgagcaacgc tcttgccgta ctctgctccg
     1141 atatccgaaa gcactttatt tgtgaagaaa tcgtttcttc cctcagcaaa cggtccccat
     1201 gacataagct gtgtattgtg ctttgccata tactctctta aaaccttctg ctgctggaaa
     1261 acgtgtgttt ccatctggtt tacggcaggc tttacctcgg caaaatgctc tatgtcaaga
     1321 tagcggtcgg ggtagaagtt tgatacgcct atagcgcgca ccttgccctg cttgtacgct
     1381 tcttccatag ctctgtagct tccgtaataa tcaccgaacg gctgatggat aagaagcagg
     1441 tcaacataat cggttttcag ctttttaagc gactcgtcta ttgacttcct tgcattttca
     1501 tagcccgcat ttgttatcca tatcttagtt gtgatgaata tctcatctct tgcaatgccc
     1561 gactctgcca cagcgtcacc gacacccgcc tcattgccgt aagcctgtgc tgtgtctata
     1621 ctgcgataac ctgtattcag tgcgtcaagt acgcactttt ttgtttcctc gggaggtgtc
     1681 tgatacacac cgtagcccag tatcggcatc tttaccccgt tgttaagcgt tacaaattcc
     1741 atagtaatat cctcctttga tcattatatc catttttcga tctgtgactt gctctgcttt
     1801 acactgcttc cgtgtatcgc aagtcccttt tcgacctttg cacccttgca gtgagccttt
     1861 atatcacttt cgctcctgcc catgccgcta ccctcgtgag tgcacagcgg ctttattgtc
     1921 ttgccctcaa agctgaaatt atcaaggaac gtaaacaccg ccataggcac agttccccag
     1981 tagttaggat agcaaagata gatctcgctg tacttatcta tgctctctgg catacttaca
     2041 agctcgggtc ttgcgtcctt tttctgatcc tcctgcgcct gcgctataca ttcgttatag
     2101 cctgccgagt acggctgttt ctgctctatt ttgaacacat ccgcaccggt aagctccttg
     2161 atatagcctg cggcgatctc cgtatttccc acagggagca ttttcagctc tccgctgaca
     2221 tagttttcat cggctcttga atagtatact ataagtttat ccgacattgt gaccaccctt
     2281 tcgtattttg tacttacatt ttatcacatt tccccttgac acggaatatc cgatatgtta
     2341 tcatcgtatt aaccaaaagt ttatactcct tatccgaggt gaattttatg tacgacccga
     2401 caataaaaac attcataacc gttgccgact gcggaagctt tacctctgcg gcatcaaagc
     2461 tctacatttc acccaccgcc gtaatgaagc agataaacac gcttgaaagc cgtatgtcgc
     2521 tgaccctgct cagcagtaat tcttcgggtg ttgagcttac aaaggcagga aaagtcattt
     2581 accttgcctc aaaggacatc atagaacgct gtgaaaacgc aatacaacag gcaagaaaag
     2641 aagaagaaaa gtacaataaa actttctgcg tcggcacatc gcttttaaac cccgcaaaac
//
LOCUS       DAWAOG010000274         2698 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4890_length_2698_cov_2.278093, whole genome shotgun sequence.
ACCESSION   DAWAOG010000274 DAWAOG010000000
VERSION     DAWAOG010000274.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4890_length_2698_cov_2.278093"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    419..428
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    798..807
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1542..1551
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          716 a          660 c          486 g          806 t
ORIGIN      
        1 gaggtacaga gctctgatct tgtcggggca cagctcatat atagcaatac cgcacatcag
       61 atgttcaagc atagttgtgt atattgtttc ccagttataa ccgggcttga aacgtttctt
      121 aagctcgcct atatccctgt ttaacggttc ggtccttgca tcgatctcat tatctgtcat
      181 gatattctct ccgttgtttc aaaattgttt gggtgcaata gaatgatcaa acgtatcaaa
      241 agccgctttt gcggcggctt ttgatcactt tatataaaac tgctgcataa atcttacaca
      301 ttggacgtac tcgtttgcaa gttgttgagc gtcaacattg tattttttta tgaactcctc
      361 gaccttatcc cagttcgcgt gctcatagtc gactaccagc ctgaacacat ctccgaacnn
      421 nnnnnnnntt cattatatta cctatatccc gttcgtcaga agtgcctgca actacggaca
      481 taagtcccat aaggtacatt tcttttctgt gtatgtactg ccccggcatt acctttgaga
      541 tactctcaca gaactttgct ctgaacaacg caagccttat cagctcatcg ggagtgtcct
      601 tctgcaggcg ctgtagcccg acaagatata tccattcttt aagcttttca aagccgagca
      661 gcacaagtgc ctgatgaata gtcgatatct tgtttccggt attgccgtac attacgttta
      721 taagtcttaa cagccttata gtcagcacaa catcggtcga tatcaccgac gctatctcct
      781 cataatcagg ctcgggcnnn nnnnnnntat atgcaggaac gtctttgcca taggcgtatt
      841 tgtcctgtgc gtcataagca gaggcttcgc aaagaaatat ccctgcatat atgtacaacc
      901 cagccgcttt gcatactcga cctcgagctg tgtttccacc ttttcggcga gcatcgtctt
      961 attatatgca atacactttt ccgctgtgcg ttcaacagcc tcacgcgagg tgtggaagtc
     1021 aagctttact atatccgcaa gctcaaacag cgcctttgta ttatctccgt attcatagtc
     1081 gtcaagcgcg agcagatagc ccattttctt aagctctata caccgtctta ttatctcggc
     1141 atccgccatt acgttttcaa gaagctccac tacaagtata tcgcttgata tcatcttcgg
     1201 aactccgcgt ttgataagat tgcccgtaaa attgacaaac gcttttttgc cgccgagaac
     1261 actcgttatg ttatccacaa aaaatgcatt atttattaca tcggcggtgc tcacatcacc
     1321 gttattcccc gtatatgcat tgactccggg ctgatttctg taaagcagct cataaccaaa
     1381 tgtattgtta ttcacatcaa gtataggctg acgtgcaaaa aatacgttct ccacagagat
     1441 cctctccttc gtgtttaatc cttacttaat agtttacatt attttgccgt caaagtcaac
     1501 aataatacct atttttcgcc tttctgacag agttttttgt annnnnnnnn natacaaaat
     1561 gtatgtaatt ttttacacat ttcattctgt atttttcgct gaacccgcag tcggcagacc
     1621 tgtagttccg gcgaaaaaat taacagccgc aaatctgcgg ctgttaaaaa ttcattttac
     1681 ttatactgtc gttttactgc actgagcgtt gatcatcttg tatccgagta tgcacacagc
     1741 acttacgact gcggcaataa gaagattata cggcagaacc gaaaagaacc attccatatg
     1801 cgatgccggt cctccgacat tcataacatt ataagggtac ttatatgcgt ggaatatgca
     1861 gatagcagca ttcacaacaa cagtaacacc gataaaccgt ccgaagctgt gctttataaa
     1921 ggtaaaaacg ccgaaagcaa cagccaatgt gataaggaat gatgcgcaag ctgaatagat
     1981 atagaaatca agtctgtccg gcatagtatc ttgcgccacg cagagccata cgcctgcggc
     2041 ggcaacgatt gcggaaaaga ttatttttac cggtttcata gtaatacctc ccttgttatt
     2101 tatgtgtttt gataaaacac ttttcttaat acaagtatat cagaattttt tcatatattc
     2161 aattgacata ttagacaaat aactcttgca ttattttaac aacatctaca acacaaaagc
     2221 ggtatgccga atatatggct accgctttac ataaactcat atcacccttt gaaatagctg
     2281 tacaacctgc acataagcat accgttatac agctttctgt tcttatctgc tttcttgccg
     2341 aaaagctcct caaactcacc gtccggagtg ataacgaaca atcttcttcc gtctgtcggg
     2401 agcattctct tacccatgac tctatacagc tcatgagcct gctcctcgtc aagcatacgc
     2461 tcaccgtaag gcgggttaca aagcactgcc gtacagccct caggatatgt aaactcggca
     2521 acatcacgcc ggtttacctc gatatattcg cccacacctg ccttagccgc attgtcacgc
     2581 gtaagtctta ccgcctccgg gtcgatatca aagccgtaca gcttaaagtt ttcgggcttt
     2641 ctgatattag ccctgagtgc ttctctttcc tcgtcccaca gctctttggg caggaact
//
LOCUS       DAWAOG010000275         2697 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4894_length_2697_cov_2.454201, whole genome shotgun sequence.
ACCESSION   DAWAOG010000275 DAWAOG010000000
VERSION     DAWAOG010000275.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4894_length_2697_cov_2.454201"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1056..1065
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          585 a          716 c          598 g          788 t
ORIGIN      
        1 tcgttcatag cagcaacgtt atcgcacaga atagcgaacc cgagaagtgc tatcccgcga
       61 aaagcactat cggtagcctg ctatcattgt taattactaa aacagcataa ttgaaatgca
      121 ataaaaacag attttctcca cataccaaaa aagtgagccg agcgcactat ttttagcgca
      181 tcgactcact ttttttgggt tgtgtcacaa cccctctgta tctgttaaat tactgaaagt
      241 tcttcttaat atcatcgccg aacttcttta tcttatcaaa gaagcttgta cgcttctcat
      301 aatttgacgg agtaagctcc ttttcaaagc tcagcaatgc gtccttctgc ttcttgttca
      361 gcttcttggg tacttcgata actaccctta ccatctggtc gcccctgccc tcacgctgga
      421 gcttctttat acccttgcct ctcagcctga aaaccgtacc cggctgtgta ccctcgggta
      481 tgctgtactt aacgttgccg tctattgtcg gcactgtaag ctcatcgccg aggactgcct
      541 gtgaatatgt tatcggtatc tcgcaccata catcaaagcc cttacgttca aatatcgggt
      601 ctttgcgtac cgtaatgcgt acattaaggt cgcccttagg tccgccgtta gtaccgcagt
      661 tgccctcgcc tcttacgcag agcgtctgac cgttgtctat accggcaggt acatttataa
      721 caactttctt acggcgctgt actctgccct gaccgttaca ggtgggacag ggtgtttcta
      781 ttatcttgcc cttaccgccg caacgcgagc acggacgagt agaggacatc ataccaaaca
      841 tacttctctg ctgtactctt acctgaccgc taccgtgaca gtcgggacag gtcttgggag
      901 tagtgcctgc ctttgcaccc gagccgtgac agctgtcgca ggtttcggca cggtttatct
      961 caacctcgtg cgacagaccc ttgcacgctt ccataaagct gatgtcaagg cttatagcga
     1021 tatccgagcc ctgtcttggc gcattggggt tagagnnnnn nnnnngtcca ccgccgccgc
     1081 cgaatatcga gtcgaatata tcgccgaggt ctatgccgtc tgccgacgaa aatccgccgg
     1141 aaaatccacc gaatccgccg ccaaatccgc ctgcaccgcc gccgtatgac gggtctacac
     1201 ctgcgtgacc gaactggtcg tactttgcac gcttctgcgg gtcggagaga acgtcgtttg
     1261 cctcgtttat ctccttgaac ttggcttcag cctcaggatt atcggggtta aggtcggggt
     1321 gatactgctt ggcaagctta cggtaggctt tctttatttc atcatcggtc gcgcccttct
     1381 gcaagccgag cacttcataa taatctcttt tttctgccat aatgatctta ccttcccgac
     1441 agaattatct gccgtgtttt aaagtattgc aaaatcagca atatgagccg agagttcccg
     1501 gctcatacgc aatgttatcg attagttggc atctgtgtaa tcagcgtcaa ccacattatc
     1561 atccgaaccg cctgcggcat ttgtgccttc gccctgaacg tctgtctgag cagcctcagc
     1621 ctgctgtgct gcagcgccct gttccttgta gattctctcg gctactgcgt agaatgctgt
     1681 ctgaagctct tcctgagcct tcttgatagc ctcaacgtct gtgcccttga gagcttcctt
     1741 aagagcatca agcttgggat ttacagaatc cttatcagcc tgtgttacct tgtcgccgaa
     1801 ttcgttcatt gacttctcgg tctggtaaac catctgatct gcggcgttac gagcctcaac
     1861 ctcttctttc ttcttcttat cctcagcggc gaatgcttca gcttccttga cagccttatc
     1921 aatatcgtcc ttgctcatat ttgttgaagc ggtgatactg atgtgctgtt ccttacctgt
     1981 gccgagatcc ttagcggata cgtttacgat accgttggca tctatatcga atgttacttc
     2041 gatctgaggg ataccacggg gagcgggagc gataccgtca agtctgaatg taccgataga
     2101 cttgttatcc tttgcaaatt cacgttcacc ctgtaagatg tttatatcaa ctgagggctg
     2161 gttatccgaa taagtcgaga atatctggct gtgctttgta ggaatggtct tgtttctctc
     2221 gatcattctc gtgcagacac cgcccgctgt ttcgataccg agtgacagag gagttacatc
     2281 gagcagtaac agacctgtta cttccttgtt gagaacgccg ccctgcagag ccgcacccat
     2341 agcaacgcac tcatcggggt tgataccctt gaagggatcc ttgcctgtat atttctttac
     2401 ggcttcctgt acagcgggga ttcttgtaga accgccgact aacagtacct tgtcgatctc
     2461 attcatcgaa agtccggaat cgctgattgc ctgcttgaag gggcccattg ttgcttctac
     2521 aaggtctgct gtaagctcat tgaacttagc tcttgtgagc tgaacattta agtgcttagg
     2581 acctgtagcg tctgctgtga tataaggaat atttacgttt acggaaggag cgtttgaaag
     2641 tgctatctta gccttttcag cttcatcctt taatctctgc atagcgaact tatcgtt
//
LOCUS       DAWAOG010000276         2691 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4910_length_2691_cov_2.274279, whole genome shotgun sequence.
ACCESSION   DAWAOG010000276 DAWAOG010000000
VERSION     DAWAOG010000276.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4910_length_2691_cov_2.274279"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          703 a          636 c          631 g          721 t
ORIGIN      
        1 caaatacgct cgttacaggc tatgatatta tcccgttctg ggttgcgaga atgatattca
       61 gcggacttga gcatacaggt cagaagccct ttaaggacgt acttatacac ggtcttgttc
      121 gtgacgagca gggcagaaag atgagtaagt cgcttggtaa cggtgttgac ccgcttgaag
      181 taatcgacaa gtatggtgcg gacgcactgc gtcttactct tgttacaggc aacgctccgg
      241 gtaatgatat gcgctggaca gaaacaaagg tcacaaattc ccgtaacttt gcaaacaagc
      301 tgtggaacgc ttcaagatat atccttatga atcttcccga ggatatggag ggagcagtcc
      361 tccccgaaca gctccccatc gaggataaat ggatattatc actctacaac gaccttatca
      421 agaacgttac tgcaaatctc gacagctatg agcttggtgt tgcggtacag aatctgttcg
      481 actttatctg ggacatttac tgcgactggt acatcgagct tacaaagccg cgtatcgcag
      541 agggcggaga aacagcccgt gccgctcaga acgtactcgt ttacgttatg cagggcatct
      601 taaagctgtt acaccccttt atgccgttca tcacagagga aatatggcag tcgatgccta
      661 tagttgcgga taaggacaac aaccccgaaa gcataatgat atcggcttgg cccgtatatg
      721 acgagaagct tcactttgcg gctgaacaga cagactttgc aaaggtagtt gacgcgatcc
      781 gtgctatccg tgtacagcgc aacgagctta acgtaccgcc gtcaaagaag gtaacaatgc
      841 acatcgaaac agccgaaaca gcactgtttg agggcgcaaa ggcattcttt gagcgtcttg
      901 cgggcgcagg cgagctgaca gtatccgaaa aggcagaaac aagcgacgat atggtaacta
      961 tcgttaccgc gaacgcaaga atatttatgc ctatgggcga gcttgtagac aaggaaaagg
     1021 agcttgcaag acttgaaaag gagcgcaagg cggctcagaa ggacatcgac ttcctttccg
     1081 gaaagctgag caatcagggc ttcctgtcaa aagcacccgc acagcagatt gaaaacgagc
     1141 gtgtaaagct tgctaaggca caggaaaaga tggaaaagat aatgctttct atcgagaaga
     1201 tgaagtaata attcagttat aatcttattc cccgattgaa aagtcgggga atattttata
     1261 tgattaagtg ggatacaaag caggatataa ctcaccgccg tgatgaattt acatcacggc
     1321 ggtgagcttt attatattgt attcgattct gccatagctt attaataatg ccgaaattgt
     1381 catcgctaaa ccccgaccgt tccgctatcc cacaaaaatc cgcaacatta tcaaaggtcg
     1441 gaatttgtag cggctcacag ccgcaagggc ggaatttgcc acgccccacg ggcgcttaca
     1501 gttggtcggc taagctgtag attagctttt ctgtcttttc ccagccaaga caggggtcgg
     1561 ttatcgactt gccgtaaacg gcatcgtctg tgctttggct tccgtcctca atgtagcttt
     1621 ctatcataag tcccttgaca aatccctcaa gatccttgct gtgacggcgg ctgtgaagaa
     1681 tctcatttgc tattcttacc tgctctaaat acttcttgcc cgaatttgag tggttggtgt
     1741 caacgatgac cgccatattc tgaagatttt tcttgcagta cagctcatac agcagtgaca
     1801 gatcctcata gtgatagttc gggatagtct gaccgtgctt gtttacagca cctctcagta
     1861 tagcgtgcgc aagcggattt ccgcttgtga ctgtttccca gcccctgtag atgaaagtat
     1921 ggcttgcctg tgccgcacgg atagagttca gcataacaga catatcgcct gccgtggggt
     1981 tcttcatacc gacagggata tccattccgc ttgatgtaag cctgtgctgc tgatcctcga
     2041 ccgagcgagc gccgatagca acataagaca aaagatcgga cagataacgg tagttttcag
     2101 ggtagagcat ttcatccgca caggtaaatc ccgtctgctc gatagccttt gtatgaagac
     2161 ttcttacctt gataagaccc tcaagcatat cctctttctt tgtcgggtcg ggctgatgaa
     2221 tcatgccctt gtagccttct cctgttgtac ggggcttatt tgtgtaaatt ctgggaatta
     2281 tcagtatctt atcctttacc tgctcctgaa cgggaacgag ccttgaaata tagtcgagga
     2341 caggctcttc cttgtcagca gagcaaggtc ctattataag caagaacttg ttgctcttgc
     2401 ccgtgaacac atcggctatt tccttgtcac gctttttctt ggtttccact atcttttccg
     2461 acagagggtg cagctccttt atttcctttg gtgtagggag ctttcttatc atgttcatac
     2521 tcattgcttt tctttccttt cgtttctgac gctttataca tcacacccgg aatttttatg
     2581 agttccgtat aatcaaaaac cgccctcaga tcatctctga cctgagggcg gatatatata
     2641 tccacggtac cacctcaatt caccgtacag gtgcaagcac tggtatacgg t
//
LOCUS       DAWAOG010000277         2681 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4943_length_2681_cov_2.405179, whole genome shotgun sequence.
ACCESSION   DAWAOG010000277 DAWAOG010000000
VERSION     DAWAOG010000277.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4943_length_2681_cov_2.405179"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          734 a          567 c          648 g          732 t
ORIGIN      
        1 tcggtgctga gctgtatact gactgatatg ataggctgct tcctgtcaaa aaaggaatac
       61 ggcgtaaagg atctgtccac tatcgcctat ggtatggcac tggcgcttat gctccccgca
      121 agcgttgagt attatatagt ggtgatcggt gcggtgcttg cgataacggt aaagcatata
      181 ttcggcggca aggataacta catattcaat ccagccgcag tcgctatcgc ttttttgata
      241 atatgttatc ccacgcaggt cctgatgtac cctcagctcg gtacacatcc cgagatattc
      301 ggtgaagccg gaactcttgt aagcggtatt gagagcagct ttatcaagaa cggcgctatg
      361 ccttcgctta cacctctcga gatacttatg ggacgatttg cgggtcctat gggcacaact
      421 catatattgg tgcttatagt gagcggtatc tgccttatct gccgccgtag cgtttcactg
      481 tcggcaacag taggcggcat tgccgtgatg ggtgtgctgt catatcttac aagcagtgta
      541 cagcctgcta ccgatgcggt cgtgttccgt ttcataagcg gatttgtact gttcggattt
      601 atcttccttg caagcgatcc gcagacgctt ccgcttacaa acggcggcag aatgctgtac
      661 ggtatcgccc tcggaattat tacggtaata ttccgcagcg ccgcaaatat cgaaggtata
      721 ttcgtgttct cgcttctgat agtaaatgcg ctttcgctgt accttgacaa gcttgcattc
      781 gtgatagcga ccgaaactaa acagatagca aaatatttaa agcacaacct gggctctttc
      841 gagcgtatga caaaggatgt aaagcaaggc aggacgccct cgctttcgga tacgcaggaa
      901 ataatgatag aacctgttaa ttataatatg cctcctatcg ataataaggt gacaaaagta
      961 aagcgtcaca aaaagtcggt attcgagaat atctcggata aaatatcgga tgtggcaaag
     1021 aagaaaaacg ccgcaaagga aaacaataca acgcagaaca ccgccgataa cgcggtaagc
     1081 gaaacggaga aggaggcgca aaatggctca ggcaaaacag ttgaagccgg aaaagacgcg
     1141 aaaaagtgat ttcaggttca cgcgcgatct tatagacgga cttgcaaagc agaacgttgt
     1201 gctgatgacc ggtattgtaa acgcgcccgt cataattgcc gcaacgacat tcaagaaagg
     1261 cgttgttata gcgcttgcat tcgcaataat atcgtttctg acgatcgtca cttgctcatt
     1321 cgtccccaaa aagatagtat acactctcag agtaattata tatgcgctgg taggctcggt
     1381 ctattatatc cctaccgtgc ttcttttaga gcagatattc ccgctcacgg tcgaacttat
     1441 cggcatatat atgccgctta tcattataaa tgcgcttata ttctcaaaga cagaaagccg
     1501 tttttatctt gagagcaagc tgaaaatgat gatagatgtt atattcttta taatcggatt
     1561 tgatgtggta tgtgtattga caggcggttt cagggagctt atatgcttcg gaacatttgc
     1621 cggcatacag ctgtttgatt ttaagatagc agctcttgaa tctccgttcg gcggatttat
     1681 tcttgtcggt ataatggcgg gcacattcag agcactttac aactatctgc gcaaccgcag
     1741 gataaggcag aacagtaaag agcagaacaa ggcggcggag cggacattcc gttcaagcac
     1801 cgacaacaca gcaaagcctg cggctctgac cgataacggc agcacaaagg agcagagcaa
     1861 tggataaatt agtattgttt ataaataccg ccatgcttgc catgttcgta gaaaacgcga
     1921 tattctcacg cgcgctcggc acaagcgttg cgttttatgc ctcaaggaag aaagagagca
     1981 tattcggact ggggctcggc ataacctatg ttattgtggt ttcaagctgt atcacgttct
     2041 ttatagacgg gcggcttgcc gattggcagt atttttatgt tgtcatgccg cttatataca
     2101 catttatcgt gagcatagta tatacgggca gtctgctcct actgtggaga tttctgccaa
     2161 agatattcag aaatatcaag aaatacgttc acctgtctgt attcaatgcg gcagtgctcg
     2221 gagcactgtt tcttaatacg acatatcacg gtgattttct ttcatatatg ggatttggtg
     2281 tcggaattgc ggcaggattt ttcattgctg tgttcttttt gcatattgca aatgaaaggc
     2341 tcaactcgcc gcttattccc gaggctttca gaggaatgcc gataatgatg gtatttgtcg
     2401 gtataatgtc acttgcattc tatgcgctta ccggttataa cacgggagct atgtgataat
     2461 ttcttaaacg gagatactga tatgagcaga aaaaaaggat ttaaggtaaa acgttcaagg
     2521 cgcaatctat acaaaaggcg taaaagcacc ggaagaaaga tcttcgaggg tgtgcttttt
     2581 gtcgtgatat tgggtgcgct tgtatttgtg ggatattcgg tagcatcgcc gctgataaaa
     2641 ttcttcagcg gaaacggcaa cacctcctca gcgagtgagc c
//
LOCUS       DAWAOG010000278         2659 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_4990_length_2659_cov_2.453149, whole genome shotgun sequence.
ACCESSION   DAWAOG010000278 DAWAOG010000000
VERSION     DAWAOG010000278.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4990_length_2659_cov_2.453149"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          633 a          638 c          614 g          774 t
ORIGIN      
        1 ccttacgccg ctttcgataa tgctcaggtt ctcacttttg cggctttcgt tgctgtttgc
       61 tatagaggcg cttactattg ctatcgcaga cgcagaaagc gccgcagcaa cgaccgcatt
      121 ccgaattaag taaaatgcac ttttacgctt tctcattttt gtcaccctat aaccgtcata
      181 acgcccatca gcggtatcat tacagacagc agtataaatc ctacagctac tgccattatg
      241 ataaccatag tcggctcaaa ccttgcaacg gcattctgaa ctaccgccga aacctttttc
      301 tctgcgttgt cggcgatgac cgccattgtt ttatcaagcg tacctgtgct gaatgctatg
      361 cttatcattc tcatattgac agccgagaat atgcctgttt cgttcagtgc ttctacaaac
      421 gccttgccgt cagtcatttt cttgcggcat tcggtgagct tttcacgcat atagtcgttt
      481 tcaagcaggt tttcgcacat atccagtgac tcgtcaatat ccataccgct tgaaagcgtc
      541 atagagagcg ccgcggaaaa tcttgcggca tacagagttt ttgccgtttt tgtatatgaa
      601 accgcacgaa ttaccgtgtt cctgaacggt cttacaaaat tgtaaagcag cacaccgagt
      661 attatcacaa acgcaagcac tccgattatc agtaccgcta tattgcttat aactccgcct
      721 gcgttcatta tccactgtgc actgtcgggg agcgtgagtc cgagctgtgc gaaaacatcc
      781 gcaaatatcg gcagtacata taccgacagc agaatgatta ccgcaaatac gactatcaga
      841 agtatagcgg ggtaggtcac cgcactgcgc acagatgtgc ttatctcttc tataccagcg
      901 taatagtcgg ccagtgacgc gaaaactgtg tcaagtctgc ctgtcttttc tccgacctca
      961 agcatcttta taaaatagtt cggaaagacc tctgttttgg caaacgcctt tgttatggag
     1021 ctgccgtgct ctatagagtc gagtatcttt tcaagcagag cagccttgtg cttatcttca
     1081 tcgtcctgaa gaatgacaag tatcccatcg gttattgtaa gtccggagct tacgacagac
     1141 gcaagctcag agcagagtat tgaaatatcc tcatttgaaa ggtaatgctt cttgctcatt
     1201 tttccctccg tatcaatagt aatacatatc ggaatagtga cttccgtccg atatatagct
     1261 tacaagatct ttataatcgg tatcgtcctt tgcgcctgcc gcaaatgtgg gatccattct
     1321 gttccagctg ttgccgtcaa agtagatata tccgtctatc cagccgcttc cgtcaacata
     1381 gacgcttatc catgcatgat atatatcgcc cgcatagccg acaacaacct ttgtaggtat
     1441 cttctgcgag cggagcattg ccgtcataag cgaggcatag tcaaagcata tgccgctctt
     1501 gtttgcaagc gccgtgtcag gaacggggat atagccgtta gcggcatttt cggcggtgga
     1561 aacatagctg atattattta taacatagtc gtatatcgcc gctgcctttt ctatcgtatc
     1621 cttgtttccg ccgcaaagct gtgatgcgag cgccacgcag gaagagcctg cgtcatacat
     1681 acagaactgg ttcggtgtaa ggaaaggcag gaagctgtct gatacagata cactcagcga
     1741 ctgcttgaac atgaccgtac ccttgcccgc gctcgtgacc ttcaatacac tgaccgtata
     1801 attgccgctg ccctctgtca gcggtatcac ctcgtaattc cccgagctgt tgagctggaa
     1861 tgtatagcgg acatttatgt catcggcgtt tacaagaatt ctgtaactgc cgtccatagc
     1921 cgttgaaagc tttatcatca gatagccctg tgacgcattt gacgcgtcta tcactgcatc
     1981 atcattttga tataccgtca ccgcgtccgc tgtgggagtg attatatagg acggtgatgg
     2041 ctcggtctgc ggaggatctg taacaggcgg ggctgtggca ggaggttcgg tttccggggc
     2101 aactgtagcc ggtgggtctg tagcaggagg agcttttgtc gttacagttg ttgtgtcagg
     2161 cggagcggtc gtagtctgct ttggtttgac tgtagttgtt ttagagggct gctccgatgt
     2221 tgaagttgct gttgttgatg tcggtgctga agtcttaggt gttgtctgtg atgtcgcggt
     2281 agcttttatt acggttgtag ttgccgccgt gcttgtgcct gatgacgatg atgagttact
     2341 gctgtcgccg ttgcagccgc cgagaacggc acacagcgca attattccgc ctgcgatgac
     2401 cgcagtcagt ctatagcttt tcatatgccg ccccctttcc gaaatacaga aattacatat
     2461 aatttaagta tagcacaaat gagagaaaat gtctattaaa tataaaagaa aagccgcgat
     2521 ttttttatcg cggcttcagt ttgtagaaaa agtatgtttt tgaaataaac ttctgattta
     2581 ttatccctat cccctttaag acactctgat ggctttgcat cgtgcaacag tggtcgtttg
     2641 ttgtcggtgc gtcctgcac
//
LOCUS       DAWAOG010000279         2647 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5019_length_2647_cov_2.293981, whole genome shotgun sequence.
ACCESSION   DAWAOG010000279 DAWAOG010000000
VERSION     DAWAOG010000279.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5019_length_2647_cov_2.293981"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          743 a          502 c          677 g          725 t
ORIGIN      
        1 gtccaggtat gctcgtcggt ttcgtggtcg tggtcgttgt gattgtgctc gtcatcgtgc
       61 tcgtcttcgt gctcgttctc gtcatcttcc gaaagggtag gaacataatc gataagttta
      121 acgatcttta ccttgcttgt atcatttgaa ctgactattt tgtctgccca ctgttcattt
      181 tcgccgccga tatacaggaa tatgtcgcag ttctgaattt cgattatatc ccttgcggta
      241 ggttcgtagt tgtggatctc gcttcctgcc gggagaagta tctttaattc ggcgttttca
      301 cctgctatat gctttgcaag gtcatacggc gggaaaactg tcgatattat cttaagtttt
      361 gtgctgtcgc tctcaaaata accgctttcc gatgagcagg cgcaaagact caggatcagt
      421 gcggatgcgg tgaataggga aagcagtttt ttcagtaatt tcattatatc actcttttca
      481 ttttgtgtcg tgctgttttg cgcattctcc gcagatgccg taaaatacgg tgcgtgagct
      541 gtcgatagta aaattgtgtt caacggaaat atgtgccgct atgtctttca gatagtcgca
      601 ttcaagatgg ataagtctgc cgcacttaag acatttcagg tggaaatggt cgtgacattc
      661 ttcgccgccc gaatactgat agcaggcact tttgccctcg ctgaggatat attttcttac
      721 aaggtgctgt tcgcagagct tttcaaggta gcggtagacg gtggttttac cgacctgctc
      781 gggtttcagc gcatcggcta tgctgtcggc ggtgaggtgg ctgtcggcgt tttcttttat
      841 atattcgagt atcagctcgc gctggcgggt gttgtatgac actttgatca ctcctcggta
      901 attcttgaaa atgaaattga aattcatttt caaatcatta gctattatac actcgtattt
      961 tgtgaatgtc aagaggtaat gagaaatatt cttgaaattt atagtgaaat gtgataaaat
     1021 caaaatacat ggtcgaatgc gaggtgtagg tatggataag atacgcataa caagagatga
     1081 aaacaacgct gttgtgctgc gctttgacaa gcgtgacgac tgcgtaagct atactgtata
     1141 tttcagaagg gaaaacggca ggttcagagc gcttataact accgagaaaa cggcagtgaa
     1201 ggtaaatgcc gtgctcggac tgtgttattt cagggtaacg ggacttacag aaaacggcag
     1261 aacggtgaat atcggaactg tcgatacttc ttcgctgctg aaaaagacaa gctttatcac
     1321 tatgggctcg tacaatattc agatgataac ggaaagaagc cctaagttct ctgcggataa
     1381 tacattaagg cggatttcgc cgctgacggc attcttccct gaaaaggttg acgaaaagaa
     1441 cattgacagc ggcaagaaag cgtttgaata tatagagaag aataagtcgg acttttttat
     1501 atttgacttt tacggcacgg cggtacacgg acttataaag gcggaaaaca catttctgac
     1561 gggcggcata gacggcaacg aaaagcttgg cgaaaagctg cctaacatac tcccgccgga
     1621 ggtttatcag ccgctggtcg atatattcgc aaaagagata cttaagctgt atccgaacga
     1681 taagataata cttgtaagga cattgtcgcc cgagttctac ggactgtcgc ggcaggtcag
     1741 aaaaagtacg ccgaagaaca gacttaacga gttcctttct aagatggagg cgtattttat
     1801 caagctggta caccctgttg ttatagatct ttcgggaaag tatttcggcg accttgcagt
     1861 caagggtgac ggtaaggagg ctgtatttga cagctactat tttgctgact gtgaaaaggc
     1921 gcttgatgaa ataatatccg gtgaaccggg aaaattgtac aaggagcagg atatcgattc
     1981 aaggctggat cagttgatgt gttattatga cagtgcgtgc tcaagaggga tacttacggt
     2041 gctgcttgac aggaaagagc ctgtggatac gatcatgttc catacatcga gggagtttat
     2101 tgcggagaac agagcggagc ttaaggatat gattggacag cactattcaa gtgttaccga
     2161 tatatacaga tattacgatt tcggcgacaa tatcgagatg aaaaatgctg tgaaagtgat
     2221 agccgcattg gaaagcaata ctttgcagaa cgtgacgcac ggcgagctta taagactgct
     2281 tgacagacaa tacaggataa agcgtccgat agcaaacttt gtaagagcta cacttagcgg
     2341 tgcactcgga aaagaagccg atataaatga gcagaacctg cggtttatga caagacttgc
     2401 gtttgaactg tgggagggaa gcgaccctaa agcaatacct cagaaaatag aagagtatga
     2461 gagaatacac aacttcacgc tgatagatat gtggggtaca ggcgttatca agagagcgct
     2521 cgctcaggca aagcagataa agatgaacac tgttataaac ggggagtcgt ttgtatgggc
     2581 atttgacaag ccgcattcaa tcgaagaaaa gagattttcg actgcggaca gatccggagc
     2641 aaaggcg
//
LOCUS       DAWAOG010000280         2632 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5059_length_2632_cov_1.803648, whole genome shotgun sequence.
ACCESSION   DAWAOG010000280 DAWAOG010000000
VERSION     DAWAOG010000280.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5059_length_2632_cov_1.803648"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2255..2264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          821 a          537 c          670 g          594 t
ORIGIN      
        1 ccctcatttt tatacgccgc cgtgagtccc ttttttaccg aatgtctttt tttcgggata
       61 gggcacgcca tcggcattcg gtacggcgta cggataaaca ccgcggtatt cgtctgcggc
      121 gtttttgctg tcgggatcgg caggtatcag tccggtgcag tcggaggacg aacagctgcc
      181 gcagaaatta tcaagtataa ggtcgtccat tgcactgcgt tcttctggtg tgtcgggaac
      241 aggctttttc ataagctcgc attgctgttt taagcttttg ttatccataa ttcaattatt
      301 cctttcagct tgcacattgc gaaattttat ctgcgtgtat agtatgctct gtttgccgca
      361 acaatataac agcagtgagt tgtaaaagca tttttacagc acactacaaa ttcgtacaac
      421 aaaattatta actttttgtg caaattttaa taaaaaacct cttgacaaag tttgaaaagt
      481 gtgatatagt gtgtttagca ctcagagagg tagagtgcta gcagagaaaa aatgtgagtg
      541 ctaatgagcc ggaaacagcg gaataccgct gaaagaaggg ggtaggaaca tggaaaaccg
      601 tacactcaga atccttgaag cgatagttga cgaatatata cgcacgggtg aggcggtcgg
      661 ctcaaagctt attgccgaga agcttgacaa cgcggtatcg agtgcgacta taagaaacga
      721 gatggcggcg cttgaacagc agggctatct tgaacatacg catacaagct cgggacgact
      781 tccgacatat cagggactga ggctttatat agaaaaggta atgtcacctg cacagttacc
      841 cgaggaagaa aaagaagaga tcgaccgtat atttgacaat atcggcggag cgacagacga
      901 tgagataatc gacaatgcgt ccagggcgct tgcggatatc acaaaatgcg cgatagtttc
      961 taccaacacc gtaaacaagt tttcggtcat aacaaaggta gacgttatcc cgacaggcaa
     1021 gagaatgtac gttctgcttc tgataacctc agagggcaac ataagaaaca gggtttgcag
     1081 actgagcttt gatcttacga acgagcagat ggagttcttc acgcagttcg taaatgagaa
     1141 cctcaagggc gtaaatcttg atgagctgtc cgatgaatac atcgataagc tgacgcaggc
     1201 tatgggcgga tatatgatga cgttatcacc gcttctcaaa gcggtagttg atatatcggc
     1261 tgaaatgcag cagcatgtgg agttcagcgg agagaaaaat ctgctgacct gttcggattt
     1321 ctcaaacact cagatagcaa cgatacttga cggcaagaac gagcttacca attccagtct
     1381 gatagcggca agcttcaaca aggacggacg aaaggcaggc actttcggag ttataggtcc
     1441 tgtaagactg gactacaaga agctgatccc atatattgaa tatttctcgt caaaggtcac
     1501 cgatgcattg tcgggcggca atgacgaaga agaaaagaac gaagaaaagg aggcggattc
     1561 agtttgagta agaaggaaaa ggaaaccaag caggaaactc agcagaccgc agacacagct
     1621 actgaagagg tagcaggaaa gaccgaagag gtgacagaag aaaaggctga aacaaaagcc
     1681 gaagagatca aggtagatcc caaggatctt gaaattgccg accttaagga taagctactg
     1741 agaaccatgg cggaatttga caactacaga aaacgcaccg ccaaggaaag aatggagctt
     1801 tctcccgaga tcacggcaag aaacttaacg gagtttttac ccgttatgga taatctcgac
     1861 agagcgcttg cggcggagtg caaagacccc gactacaaga agggcgttga gatgatacat
     1921 gagagctttg ttacggctct tcagaattta ggcgttgagg tgattgaatc ggacggcgca
     1981 cagttcaacc cttcgtatca tcaggctgta cagcaggttg aggacgacag caaggaagag
     2041 ggcactatag ccgccacatt ccagaaaggc tataagatcg gagaaaaggt tctccgtttt
     2101 gcgatggtag cagtcgtaaa gtaaaaaaac aagcaaatgg gcttagatcc cgaaaaataa
     2161 agggttcggt atgaacctta cagcaaggag ataattatca tgggaaaaat aataggtatt
     2221 gacttaggta caacaaattc atgtgtagca gttannnnnn nnnnatcaca aacagcgagg
     2281 gtgcaagaac aactccttca gttgttgctt tcacaaagga cggcgagcgt cttgtaggtc
     2341 agctcgctaa gaaccaggca gttacaaacc ccgacagaac ggtcatttcg atcaagcgtc
     2401 acatgggcag tgactacaag gtagacattg acggcaagaa gtacacacct caggagatct
     2461 cggctatgat ccttcagaag ctgaagacag aggctgaggg ttacctcggt gaaaaggtaa
     2521 cagatgcggt tatcactgtt cctgcttact tcacagactc acagcgtcag gcaacaaagg
     2581 acgcaggtca gatagcaggt cttaatgttc agcgtattat caacgagcct ac
//
LOCUS       DAWAOG010000281         2632 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5060_length_2632_cov_1.759798, whole genome shotgun sequence.
ACCESSION   DAWAOG010000281 DAWAOG010000000
VERSION     DAWAOG010000281.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5060_length_2632_cov_1.759798"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          920 a          544 c          572 g          596 t
ORIGIN      
        1 gaccacagct atcacagcag agaccaccac gaagccacag gaaacaaaaa gtgctgaaac
       61 taccactacc accacaaaag cggtaacaac ggcaaagcca aagccggcaa cgacaacaac
      121 cgccgctaca acaaagccca cacccgaatg gacggagagc aaggcaagcg gtacaaagta
      181 cgtcaacacc gactgctaca gccgtaagaa agccgtactc ggcgctgaca cagttaagct
      241 gtacagcgtg aacgacaagg taacagtaac agccaagacc gatacaggct acttcaaact
      301 ttctgacggt agctttatac acggtgacta tctgagcgat aataaggtag ctcagactgc
      361 aaccacgacc acaaagaaag ctgaaacacc taatccttca cagacaacaa cgaagaaaca
      421 ggaaagcaag ccgaagtcca tatatgagta tccgttcgac cttaaagcaa tccgacaggt
      481 aattatagat gatgcagtca ataattatgg cttggtatac ggagaaaaaa tagtcaagga
      541 cgaaagttcg tggagtgccc caacagtcat ttctaaagat atgaatacaa atatcataaa
      601 aagaaatgta aacgaaattg cagaagccaa ttttatttgg tgccaaatga aaaaaggcga
      661 ttactttaac gtatatatcg aaaccatacc ggatttaaag aaagatgaaa acggtaacag
      721 cattgagggc tacgcaatat tcttcttaag ataattgctt gactttttct ccagaagtgg
      781 tataataaaa acaaagaggg aaccatagac ggtcgctccc tttgggtaat tgaaaataat
      841 cgcctttagt tagcagacca caaggcgatt atttcttttt tatgaaagat aataatccga
      901 gaagcataac ggtaaatccc gtcaggaata taccaatctg aatgagatta tccagtgtta
      961 tgtacatcaa gggcttcacc tcctttcaca atgtaataac attgtttggg agcgtgaatg
     1021 accgcctact gtccaaggtt tccctcactg aaaattataa catatgttga cgctatatgt
     1081 caacgaaaga atattaataa gagctttgaa agtattgatt caaagctctt tttctttttc
     1141 tgaaagttaa ctgaatatga aaatccaaag cagtccgcac ccgtaggact gcttttttat
     1201 atatataaat acaaaccaag aaaggacaaa ggttatgttt aaaaacaaat tcaaaaagct
     1261 ggcaggcttg ctccttgtgg ctgtaatgac attagccgtt cttcccattc agacgcttgc
     1321 gtcagaatgg ggcaaaggtg acactgtata cgccgcaatg ctcggtgact atgtcggctc
     1381 ggacggaaag tcttacggcg ataagcgtaa ttacacattt atgtattaca agtctgacgg
     1441 tagcgaaggc acatcaactc gttatgcttc aactcacgca aaactcggca taagtaagaa
     1501 cggtataaca cagcaggcta tctgtatcga agcaggcgtt gcttacaaca ccggaagctc
     1561 ttatgtcggc gaggataaca gcaatacata tttaagaaac cttcctcgtg atgtacagca
     1621 gtcgcttaaa tatgctctcc tctgcggctt taacagcgac accaaaagct cacccgtaag
     1681 cggtactaac cttgacgatt tttcgttcgc aacgcaaata atcgtatggg aaatacagca
     1741 gcagttaagg acaggatata gcaagggaga tctctctgcg aataagtggg gcactcctgc
     1801 aaaggtgtac tatgaacagc ttcaaggcag accagccgaa aagtgctaag ctgaaaaagg
     1861 taaaatcaca gaaagagctt gaacagatta acaaagagat tgatttaagc ggttacaaat
     1921 attctgacct tgcgaaagac aaagaattcc gggacgataa atcgtatgat atgagcaaat
     1981 acagtcagag tacggtacag cttataaaca cactcgttaa gcccgaaatg ccgacatacg
     2041 actatgacga ggaaaccgta acaattgacg gtgtaaaggc aaaaaagcag atacttactg
     2101 tgcataagga cagcaaaact cagatagtgg agtacacctg ttacggcgaa ggtgacatat
     2161 atcttccgag attgcttgca ttatatcagg cggaaacatt cagccgtttt gaagatatgg
     2221 cagaaagtga cgcaagcgga cttgacaacg atatagcaag tgctatcgag aacgtaagct
     2281 cagacgaaag cggcggtgca acggcagatg gttttgacgt tgcgtgtctt aacgtgttcc
     2341 aggcacagga gataggacag atattcgaga gagcggctct tgagggtaaa ttttccgcaa
     2401 aagtcaatac ggaaaagaac gatactacag aaaaactgtc aataacggta aaaacaccgt
     2461 cggaagatga ctggtacgaa atgttcaatg tggacgagaa gtacagggat tacaccaaag
     2521 aaaatacgca gataatcgag cgtatcttat ccgacgcaca gataaatgat ttgtacatat
     2581 cggtagacag tacagctcaa cgtgcattgt tcgtgtactt tcaaggcttt tt
//
LOCUS       DAWAOG010000282         2632 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5057_length_2632_cov_1.921226, whole genome shotgun sequence.
ACCESSION   DAWAOG010000282 DAWAOG010000000
VERSION     DAWAOG010000282.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5057_length_2632_cov_1.921226"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2131..2140
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          712 a          572 c          533 g          805 t
ORIGIN      
        1 ccgttttcaa ataccacaga cagatcgttt atatcgtgtc ctatgtcctt gaaatgctgt
       61 tttacgctgt cggtgatgtc gatctcacgc agtgtaacgc cgagtgcttc gcatatcttc
      121 actgcattgg attttgtacg tttggttgtg ccaaagcaag gcattgttac cgctacgata
      181 tcggtcatcg gtctgtcaag gagcttcatc gcattgacgc atacaagaag cgctagtgtg
      241 ctgtcaagtc cgccggaaat accgataact gcggtttttg cataagtatg tgaaattcgc
      301 tttttaagtc cgtgtgcttg catcgaaagg ataagctcac atctcttatc tgtttcatcg
      361 gcagagtaag gaataaacgg tgtgcgtgaa acaaaacgag tgaggtttgt ttcggaaacc
      421 gccatatcaa accatatggt ttcggcttcg tgcttattgt ctgtgctatt tctgaatgat
      481 gtgttctttc ggcgctcaaa cgcaagctta tttacatcta tttcgctgat aacataatcg
      541 tttgagaaaa gcacactctc tgcaagtgtt gtgccgtttt ccgctattat attatgacct
      601 gagaacacca gatcctgtgt gctttcgccg tcgccagctg agcagtaaat atagcccgat
      661 ataagccttg ccgattgatt aagaacaagt gaacggcgat attccgactt tcctatcatc
      721 tcgttgcttg cagaaagatt aagaatgata tttgcacctc tctgtgcaag atatgtcgag
      781 ggcgggcaag gtgaccacag atcttcgcaa agctcaactc cgaatgaaaa ttcgtgcata
      841 tcgttacaag tgaacacaag tcccgcaccg aaatacgcgc tcaatatatc gtcacattcg
      901 ccatcgccgt ctgtattgaa atgatttaaa taatattcgc atttgttacc gtcccatgcg
      961 gtaaagtggc gcatctcata aaattcgttg taattcggaa gatactgctt tggaacatat
     1021 ccgagtactt ctccgtgata ggttacagcc gcacagttat atagcttgct ttcaaactgc
     1081 atcggcagac caactacggt tatcatatca agctttgata actccttcag aatagttata
     1141 agtgcattct tagcaccgtc caggagcgtt gtctggaaga atagatcctg acaggtatag
     1201 cctgtgatac aaagctcagg aaaaaccgca agccttacgc cttttccggc aagctccttt
     1261 gcaagtgaaa ctatctgttt tgcattgtta ttacagtcag cgacctttat ttcgggtgtt
     1321 gccgccgcaa cttttataaa tccgtctttc atattgttct tttcggctta aggctcaaaa
     1381 ttcataattg cctgtcattc cgccgacctt tctgtttatc cagtttaaaa tatcatcaaa
     1441 aactatttct ttcatagttt cgttcacaag ttcgtgacgt gcgcctgggt agagctttat
     1501 atccacgtta cagccttcat ctataagctt tgcatatacg tttctaacgc ccttgccgta
     1561 tccgccgaca ggatctgccg ttcctgccat aaatataaca ggtagttttt tcggcacttt
     1621 ttcagcccat tgctgacttg acaccttgcg aagaagagtg gtaaggtcaa caaagccgct
     1681 tgcggtgaag acaaaattgc ttttttcttc atctatatat ttttttatta cttctctgtc
     1741 gtgagtgagc cagtcaaaga tagtttgtct gtcttttatt ttatcgttat acactccgaa
     1801 tatgagagtg ttgagcttat cgctgcggaa acgttcaccc ttgatggaca ctacagagcg
     1861 ggtcgctctt acaccgatat cggaaagcga cttatcatcg cctgtaccca tgataaccgc
     1921 acaggtgagc tcattcggaa agcgtgtcag ataaagcctt gcgataaagc ttcccatact
     1981 gtgacctatc agaatataag ggagagcagg atacttcttt ttcattatca tggtcatacg
     2041 gtgaagatcg ttaacaaggt ggctccagcc gtcttttttt gcaaagtaac cgaggtcgtc
     2101 attagatttt acactgtttc catgtccgag nnnnnnnnnn accgcagact atataaccgt
     2161 gtgatgtaag aaaatccgca aaatgttcat aacgctcaat atattcgcac ataccgtgag
     2221 aaatctggat aataccctta taaccgccgt ttataataga tgtaggcagg taaatataat
     2281 aggcacaatt gtcaataccg tcagatgatt caaatgtgcc gatcagcttt tcatatgcca
     2341 tagactttct cctttcgtcg gtgctatctc atataatata ctattatatc aaataaggtc
     2401 gtgaaataca agctaaacgg caaaaatgaa gatattgcta tgctgaaatt cctgatatga
     2461 tcggaacata ttcttgatcc ggctgaatga gaaaaaattt caaaaaagtc ttgacaaagt
     2521 ggttttactg tgttataata tactagcgtc aaagagatgt gtacagcttg ttcgacacat
     2581 tatgacttat atgcgggtgt agttcaatgg ctagaatccc agccttccaa gc
//
LOCUS       DAWAOG010000283         2595 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5176_length_2595_cov_2.387008, whole genome shotgun sequence.
ACCESSION   DAWAOG010000283 DAWAOG010000000
VERSION     DAWAOG010000283.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5176_length_2595_cov_2.387008"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    515..524
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1974..1983
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          826 a          516 c          520 g          713 t
ORIGIN      
        1 cttgtagata atgcggggaa ttttatacgc cgcagcggaa gcggtcagac gttcaggcag
       61 gaaggtatgt acagtcctgt cacgcagaat gaacgacatc ttgccctgat aaaggaaatg
      121 cgcagagata caaagaatgc actgcttaaa ggactgtttg agagcaattt caacaacaac
      181 taccgttcgc ttatagtaat ggcaaatccg aaatcaataa ttgattacag gtacgctcct
      241 caggatataa aagacaagat cgtaaaggct gacggactaa tcagaaagat aaacgagata
      301 tgcagtacaa gtaatcttcc cgatatgtcg gacaagcaga tgaaaacgct tgccgatttc
      361 tttatggagc atcatacggc taacaagaca gattataccg ccaaatatca gaatatgata
      421 aacaaggaag ccacaaaaga aacagttgct ctagttgcca cagagtcaaa accgactgct
      481 gttgaaaata ggtatacacc caaaagcaac ccaannnnnn nnnncaaccc aactgtatgt
      541 tcgcccgaca caagcggatt tgtcggtact tcgggagcaa atgcacctga tacacatatc
      601 gacaattatg aaaatctgcc gatatacaag gcgctcaggc agtacagata cgaaaagagc
      661 aaagctgaaa atatcaaggc gtattatgta ttcaaaaatg atgagctgat cgagataatc
      721 agacttatgc cgaccgctcc gggcgagctt agaagtataa gaggttttgg agagggaaaa
      781 atacagaaat acggcattga tattgtaaat atcgttaaca gatacagatg aattttcaga
      841 tataaaaaat aaccgtccgg gtttaaacct cgggcggtta tttaatttca ttattctcat
      901 atttcataat atcctcgact ttacagtcaa gtattacaca aagatcatcg ataagctgag
      961 tggtgactcc catattatgc cgcagtctgt ttatcgtgct gctgcttata ttatacttat
     1021 tgataagcgc ataggtcgaa atccctcgct ttttcattgt atcccaaagc ggtgtatata
     1081 ctatcatatc tgcttctccg atccggctca gaataaatta tcatttaata ttataaatta
     1141 tgtccgacta cttgaacatt gtcgaatttt tgactataat ataaaatggt tcttattctg
     1201 acaataaacg gaggcaaaac atggtttgta caattttctc tgataaacac gaagtaaaca
     1261 gcatttataa aaatgattac gaaaaatata tacacaggtt gtagaaaaag tctgtttttt
     1321 agaaatgtag cttattctca aaccctaacc ccaaacccct tcccctcggt gaaaggctgt
     1381 gtatgcttgg ttggctttgc atcgtgcaaa gcccttgtgc atacacgccc ggcatccgtg
     1441 ccggggctga atgtggggct gccgccctca ccaagcaagg atgcttgcag tcagtcgcac
     1501 acagtcgctg cacgatgcag cgacaccaac gactgacgac ctgcattgtg gctacgcccc
     1561 aaaccccatt tttaaaaatt atgtaggttt atcgacagtc tgatatacag agtatcaaga
     1621 aaaaaattat agaaacggta tgggaaatgt attgtaacgg ttatgataca tttcaagtca
     1681 attgtgaata cggcattcct ttatggacgg cagaaataat cactgctttg aaaatgtaca
     1741 attctatatt tcttgatatc tttgtaccat atgaagagca atgcaaaaat tggtcggaag
     1801 ataaacgcaa cagatattat gaatgccata agaatgccga ttctgtctca attgtgaata
     1861 tgcattacag caaagaatgc tataaaattg cagaagagat tatgatacgc aaaagtgata
     1921 ttgttatatt attcgggttc tcagaaagta tacggtacta ttcggatttt tatnnnnnnn
     1981 nnnaaattat ataggtttat cgacagtctg taatgcccgg tcaacgaccg ggcaattttt
     2041 atagctttgt aacaatatca taaaatccgt tcagatctct gacagtgtat ttctgcccga
     2101 aatcgcggtt gttatcatat ctgtcgatat atacagacgt acagccaaag ctctcggcta
     2161 ccagtacatc ttttctttcg tcaccgatga atatcatttc atcgggcttc acgccaaaat
     2221 gtaccgctat atcgacaagc ccctttatgt tcggcttgcg gtatctgcac gaggttgacg
     2281 aaacgtaaaa atcgaaatac ggtataagct caggaaaata gcttttgtgc agttcgtcgg
     2341 gcattcctgt ggctacatcg gtaagtgttg ctgtctttat accaatggta cgcagttggt
     2401 acagaaccgg tatagtttca ggatatgtgt atgcttcaag cgggaaagcc tcgaaaaagc
     2461 aataagcaat tttgtcggtc gaaatatccg taccccactt ttgagctatt tcgccaaata
     2521 tacgcttgtg cgaataatca gcctcccgat attttatcgt aggattatag ccacgcagta
     2581 tttccatacc tgagg
//
LOCUS       DAWAOG010000284         2593 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5182_length_2593_cov_1.770686, whole genome shotgun sequence.
ACCESSION   DAWAOG010000284 DAWAOG010000000
VERSION     DAWAOG010000284.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5182_length_2593_cov_1.770686"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    302..331
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          672 a          632 c          524 g          735 t
ORIGIN      
        1 ccgcagttct caccattgta tagccttgta tgtatctgta gtagaacata tcccttgtct
       61 gattgtccgc aatattgccg atgaagcgtt ctacttctgc tttctgtgct ttaagttcgg
      121 acagtcttct tttcttggca aggtgtgtat cgttatccgg aacgccggaa atcgtgacgc
      181 tatgtttgac ataggggtac tcgctctgcg aaccctgcgt gctgtcggtt gctgccgcat
      241 cgttcagctc tgattcaagc tgcttgatac acttgcaggt gtaacgataa tctttgagtt
      301 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncagaacagt ctttgcaatc ttctgctatc
      361 ggatgtctac aacaaaaacc gcactctttt actaaagcaa ctctgtcttt cggatctgac
      421 cagtccattt ctgttttggc atacttgctc tgctggttac ggtgatagta ggctttatcg
      481 taatgtcgca aggcaacgac agaggcgctt agcataaagt aacagcccgc aggaatttta
      541 tcttcctgtg ggctgatttt tatttatgca ctcgctattt ctgctgtaac agcagcattt
      601 gccgtatttg ccgcgcatac gggacagaca ttttctgttt cgcctgcaat gaatttcatc
      661 gccttgtcgg tgctccagaa gaagcgctcc tcgccatatg tttcgataag tccgcaacgg
      721 tggaacattt cgtcaacagc aggctgtacg cacgaaaagt atatttctat ctttcttgcg
      781 gaaagactgt cgcaaagctc cttcatagcc tgtacgccgg aaatatccgc tataggcacg
      841 cctcgcattg agaatatcag ctttccgccg tcctctacat caaggtgcga aagcttctct
      901 gacagcttgt ttgatgttcc gaaataaagc gggcctgtga catatacgac ctttgtgccg
      961 cggagctttc cgtccttgtc agctatatcg atcttcgcaa gatcaacatc ggcaacattt
     1021 accgtcatat ccgaaacctt aagcacgaac atgatgactg agaaaagtat accgattata
     1081 attgcgacag tgaggtcgaa tataaccgtc gccgccattg tgataagata ctggaacatt
     1141 gcggtcttta tttttctgcc gaaaatgtcc tttattaccg caaactcgtt cattctccat
     1201 gcagttacga taagtacgcc tgcaagcgcc gccataggta tctttgacat tacgcctccg
     1261 agcaggaaca ttgacgctaa aagcaccagt gaatgaatta cacccgcaat tctcgtctgt
     1321 gcgccgctct ttatggcaac gctcgttctt gcgattgccg ctgtagcggg aacgccgccg
     1381 aagaacggta tcagcatatt gcctatgccc tgcgctacaa gctcctgatc gctgtcaagc
     1441 ttttcgttct tcattctgcc tgcactcgcg ccgcataaaa ggctttcgat aagcccgagc
     1501 gccgcaatgc ttattgcagg gaatataaga tttttgaccg tgtcgaagtc tatagccgta
     1561 atatcaagcc tgtcattaag taaaagcgtc tgcggtatct cgcctacaat gccgacatca
     1621 aagttgaata tgaagttcag tgccgttgct attataatag acataagtga tgacgggatg
     1681 atagcattga gctttttggg ataaataagc ataaacacta taacgcatac accaattgct
     1741 attgcctcga aatccaatgt ctggggcatg ttgaagtagc ttactatctt gtctatagta
     1801 gacgcaccat cgcacttaag tcccgtaaga ttatttatct gtccgaatgc tattattatt
     1861 gctatacccg atgtaaagcc tgtaataaca ggtgtcggta taaactgtac cagtccgccg
     1921 agcttaaaaa tcccgcacag cagaagtata gcaccggaca taaagcaggc gatgaacaca
     1981 ccctgtatct gatatgtagc cacaagcgag caaaggatag ccgccatagc acccgtaggt
     2041 cctgatatct gatatgaagc acccgaaagc agtcctatca caacacctgc gataagtgca
     2101 gtgataagtc ctgcggcggc agtcgcaccg ctcgatatac caaacgcaag cgcaagcgga
     2161 agtgccacag ccgcaactgt aataccggca agcacatctt ttccgaattt tcccgcatta
     2221 tagcctgtaa attctgtttt taaacgtttg aaataacgct ttaccatttc tctccctctt
     2281 tctgttcctt tgtcctgcgg acaaaaagca acctctatat tttatacctg aaagaggaat
     2341 ttgtatatat caaaactcaa caaagatttg ttcacattgg gtaaatttta agtgacatat
     2401 tcaaaatata aaagctcctt tccgaacatt tcctgtccgg aaaagagcct gtcattttca
     2461 ataatatcag atctgtccgt aaatcggagc atatttactc ctcacgctct cagatacaac
     2521 ccatattcat aactatcccc ctgatattcc tcgctgattt taccgatcaa ctgcgtaaat
     2581 ccgagctttg aat
//
LOCUS       DAWAOG010000285         2569 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5232_length_2569_cov_1.685362, whole genome shotgun sequence.
ACCESSION   DAWAOG010000285 DAWAOG010000000
VERSION     DAWAOG010000285.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5232_length_2569_cov_1.685362"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1863..1872
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          620 a          686 c          517 g          736 t
ORIGIN      
        1 cacgagctgt atcatatagc cgcccgcctt ctttatcgta aggtcggtat caacaagcac
       61 accgagcgcg catacggtag gtatctgttc gcttacagca tagtactgtg ttacgtccat
      121 tgcaatctca ccgctgatga tcggcacctg tccgacataa ggctctttca ttccgagatc
      181 tcttattacc gtaagataac cgtcagtgcc gactataccg ccgacatcaa gcttgccctt
      241 ggcattaagc ggcagatccg cataagggtt atcaatacag cacttgacat tgcctctgta
      301 gtcggcaacg gtcgttatcg ttccgatctt tccgccgccg tttattcgca gttcaagtct
      361 gtcggtttcg ttcttcataa gcgcacccat aatacaagcc gcagtggcaa gtctgcctgc
      421 cgccgctgac gctgtagccg atgtgccgtg tattctgtgt atttcgttta caatatcggt
      481 ggtgtcaatt gcactgcaaa gcgcactgcc gtccgccgat aatgctctta ctatctttcc
      541 catttattct gcttcctttg cacagcagta aagcaccttt tcgctgtcct cactgccctt
      601 ttcagttgtc atataattat atctttcaag aattttaaat cctgccttat gcagtaattc
      661 ttcaattttt tcaagcggta aagctatctc cgaaaaatcc tcatatgagc gatcgaatgc
      721 accgtcttca ttttccgtga agatatcaag cgctatatct accctgttgt cgttactgtc
      781 cttatcgaaa taattctgcc acacgcagta gaatgtatta tccgcaagca cctctctgtg
      841 ctttcttaca gtattgatat caaaaacgaa aagccctgtc gggtcgcaga aaagcgatac
      901 cgacttaaag caccgcagta tatcctctgc gctgtcaaga tggttaatgc tgtcaagcac
      961 gcatacagtc acatctatag tgccgtacat atcaagctca cacatatcct gacacagata
     1021 ttctatgtta tcgtgcggct tttccttagc gatactcagc atttcggtgc ttagatccac
     1081 cccgatgaca tcatatccga gagcggaaaa ctgcatcgac atactgcctg tgccgcaggc
     1141 gaggtcaaga agtatgccct ttttcccgcc gtgagaagta attatcctgt cgtaatactc
     1201 agcaagctcc ttatagtcga tattggaagt cagcgtgtcg taaacgtccg caaaacaacc
     1261 gtatgccgac atattatcac cctttcggtc agtctttatc cttcattctg tcaaatacga
     1321 tggagtagcc gtcattaccg ccctgtaagg atctgtttac acgtcttatc gtatctgtgc
     1381 tggctcccgt ttcttcaacg atatcgctgt acactctgcc ctcaacaaga agctttgcaa
     1441 cgtgcagacg ctgagccata gactcaagct ctcttatggt gcacagatcc tcaaaaaaat
     1501 tatagcattc ctcaacgctt ctaagctcca ttatcgcctt gaaaagctcg tccttatcct
     1561 ttgaaacaag ttttgtgttc attgtgtttc cctttctcaa aagccacggg atacagtacc
     1621 cctatgataa tactactatt ttaccacact aaagtataaa agtcaaaact tgaccctttc
     1681 atcacagatc gatttcaaaa agtcttgcgg taaagcattc gggcagtttt acggaaagcg
     1741 accttgacag cggattgaac gtatgcttac cctcgtcaaa agacggatag atacagcgta
     1801 tctttgcgtt ttccgtagcg tatcttatcg ggatattaca gcaatcgctc tttccctgcc
     1861 tgnnnnnnnn nngtccgagc gtgacccatt catcgccgat atgagataaa cctaaaggcc
     1921 agaacggcac ggaatttttg atatcgccgc ggatattctt gtagcagtcg agcgcttcct
     1981 ttacaagtga cattcgctcg gggctcaggt ttgcaaggtg tccgctctga tggatacgct
     2041 gtgtcattgc attcaccata ttgaacacga cctcttccct gtcgccgtct gtcagcggat
     2101 atgaccatat agcgctctgc tcggggcaaa gtcctgtcgg tgcgtttgcc gctatcgttg
     2161 catatcttct gtaatcgtcc tggtcgcttg tcgactggat cgaataacga ctgagcatcg
     2221 catagtctat tctcataccg ccgctcgagc agttttctat gataagctcg ggataacgcg
     2281 caaacacgcc gtcaagccaa gcaagatatg ccttttcgtg gcgcataagt ccatcgccca
     2341 cgctgtcaca gcctacctct gtgcctactc ccggctctat attgtagtcc atcttgatat
     2401 agcctacgcc atagtctttt acaaggcggt caattacgct gtctgcatag gcactgacct
     2461 cggggttgga aaaatcaagc tgataacgag aacggtcata tactcgcttt ccgtgacgca
     2521 taaagaacca ctcgtccggc actttctttg ccatctcgca gtttatgcc
//
LOCUS       DAWAOG010000286         2568 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5234_length_2568_cov_1.966574, whole genome shotgun sequence.
ACCESSION   DAWAOG010000286 DAWAOG010000000
VERSION     DAWAOG010000286.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5234_length_2568_cov_1.966574"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    746..761
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1238..1247
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          619 a          559 c          687 g          677 t
ORIGIN      
        1 agggctacat agtagaaatg tacaagggcg gaaagtgggt acgcgttgct aagatcacta
       61 acagcaatac gactacattc agaaaagcag gacttgctaa gaatactgct tataagttca
      121 gggttcgcgc gtatcatatg agcggtaaga ctgcgcttta cggtaattac ggaaatgtaa
      181 gcgggaggac tgcggcaaag taaaatccct gcagaaaccg aaaaataatg aacaaccgga
      241 aatcgacctt atagtcggtt tccggttgta tttttataat agtattactg tctgtctatt
      301 ctcacatcat attttctcat ccaccattcg atttgcaaca gataagcgaa aatctgcggt
      361 gaggtcatca gctgaccgta ccagttaccc ttgaaagagg tcgggtcatc gcacagctca
      421 agcagctttt tacggcttac tatctcggtc agacggcagt cgtttttgct taagatatca
      481 cgcattctgc gtactacctc ggcaaggtag gacggattgt gggtcttggg gtaaggcgac
      541 tttttgcgcc atagcacatc atcaggcaga atgcctttca ttgcacggcg gacaacacct
      601 gtttccctgc cgcctgccgc cttgatatcc cacggaatat tgtaggcgta acgcgctatg
      661 cggtggtcgc agaacggcac tctgacttcc agtccgtgag ccatactcat tctgtccttg
      721 cggtcaagca ggcactgcat aaaccnnnnn nnnnnnnnnn nttcagcatg aacatctcgc
      781 gcatccgtct gtccttgcgg ctgtctgtat cgagatattc tgttttacgg agtgtttcgt
      841 tacagcagga gttgacataa tcgttcggtt cgatgtcgcc gagaacgccg tcacagagca
      901 gatccgcgcg tgatgaagtc gaacaagccc aagggaagcc ggggcggtag aggatctcct
      961 cgttgtgata cctggggtag ccgccaaaga tttcatcggc gcattcacca cttaatgcaa
     1021 cggtaaaatt gttctttatc gacttgcaaa acaggtacag cgagctgtca acatctgcca
     1081 taccgggcag gtcgcgcgca taggttgaca gctcgagcgc atcggtaagg gcgggggtat
     1141 ctatttcaat gttaatgtgc ttagagccga tgaatgctga catttcgcgt atccacggcg
     1201 cgtccatatc gggttgaaaa gtactgctgt gaaagttnnn nnnnnnncct tgtaatcgat
     1261 cgagcacgtg gtcagttgcc tgcctttgct ctcaaattcc ttggcggcga ctgcgctgat
     1321 tatgctgctg tcgagcccgc cggaaagcat cgtgcacagc gggacatcgc ttacgagctg
     1381 acgcttgatg gcatcggtaa ggaggtaggc aacgtgctgt tcggtttcct ccgggctttc
     1441 cttatgcggc agggcacgga gcgtccagta gctccgcttt ttaagtccgc atcggcagta
     1501 cattgcgcat tcggcggggc agagctcctt gatcccttta aatatgccgc ttcccggctt
     1561 tcgggcagga cctaagagca ttatctgcgc tatgccgtca cggtcgatca caggctctat
     1621 catagggtgg gcgaggagcg tttttatctc agacgcgaag atcagcccgc ctgcgtattc
     1681 gtagtaaaac agcggcttca cgcctattct gtcgcgggcg aggaagagct gtttttcacg
     1741 ctcttgccat atggcgaagg cataaatgcc gttgagcttg tcgaggcagg attcgcccca
     1801 ggtgatgtag gcgttgagca gtacctcggt gtcgctggcg gtggtgaact caacgcccat
     1861 ggattcaagc tctgagcgga gttcggcggt gttgtaaagc tcgccgttgt agacgatggt
     1921 gaagcggtcg ccgccatatg ggcgggacat aggctgtgag ccgcctgcgg ggtctattac
     1981 gctaaggcgg cggtgcgcga gggcgcagtt gtcatcggtg tagaagccgc cgctgtcagg
     2041 tcctctcggg gtcattgtct gagccattct gcgcattatt gcggtgctgt cgcagagctt
     2101 gcggcaatct atccaacctg ctattccaca cattaaaaat cacctcatta tgtagattat
     2161 ggtatattat ggctgataca aaagtagtat atgcgatgaa aataatttcc gtgaatggaa
     2221 ataacaagtg gtatagaaat ggtacattgg tggtacggat gggtaatgaa taatgaatga
     2281 tgaataatga ataattgtgg tgaccgccgt cggcggtgga ttttaatttt gggtggtgcg
     2341 gtcgggggat aaagcaaatt tgcagttatg tatttatata aataataaat atgtataata
     2401 acgctcgaaa tgaatttaat taagtgatag cttttggttt tgaagtgcat tttgaatttg
     2461 atttcatatt tttttgtaaa atcggtgctg attttgcaga aaagggttga caaatgaaga
     2521 tattggtata tcattaaatt ataactgtgt ttacacagga aatgaaaa
//
LOCUS       DAWAOG010000287         2564 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5243_length_2564_cov_2.340375, whole genome shotgun sequence.
ACCESSION   DAWAOG010000287 DAWAOG010000000
VERSION     DAWAOG010000287.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5243_length_2564_cov_2.340375"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          808 a          573 c          547 g          636 t
ORIGIN      
        1 cttacattaa acggtatatg catctggcac gtcggttttg ccggcaagtt caatgccgca
       61 atggagcttt atcaggtaca cagaaacagc ttcgtaatac aggctgccgg cggcatatgc
      121 aaggatatag atttctatgc aagaatcaag actcagttct ggaaggaaat aaccagattt
      181 gcatataaca atgcggagtt acttatcgac tctatcgacg attttatgaa gggtcccgat
      241 tggtttatga accttgacgg cgagcagtca ctcaaggagc acaacgccaa gaacgaaaaa
      301 atggttccta tttcacagct tacagactac cccaacgccg gaagagatga tccgtttgaa
      361 tacaagcgca tcaataagct tcagaaactg ttttatatga tgaccatcaa cggtcacctt
      421 ttacccggtt tcatgttaag gaactaccct gaggttattt catatgactg gttctttgtt
      481 ccgggcaaaa actaccgcag aaagacactt attgccgtag accgcaagca cggcacagcg
      541 tatgtacgca aaatgtcaag aaaacgctgc tttgcgctta tcaagcgttt caataaggta
      601 agcaagaact acaagaaaaa tcacgcttta atagaaaaac agtattccga taagtttgct
      661 cagatgacaa cagaaaaatt ctggaaggag taccttggaa tatgaacaga aacaacagga
      721 taatccgcac acttatcgca gccaaaagag catttttcac caacactatg ccacccgctc
      781 ccaagcagca aaatgctgcg cctaaaaagg catctgcatc taaacctgcc aattctgctc
      841 tgacacctgt tgttcctgac cctataatac tcacagataa gcccgtcaca ataaatgatc
      901 ttaagcacag ctactgcata ggttgcacgg cttgttacaa tgcctgtccc gtaaacgcaa
      961 tttcgctcag tcgggatgag cagggttttg ttactcctgt aatagataac ggaaaatgca
     1021 taaaatgcaa aaagtgcgtc agtgcctgcc ccgttatgag cacaaaatat acaaatactt
     1081 ctaaccctaa gtgctatgca accatggctg acgatgagat aagaatgaac agctcctcgg
     1141 gcggtatgtt tacacttatc gcagagcata taatcgacaa cggaggatat gttgccggcg
     1201 caactctcgg tgaggatttc acagtacagc atattatcgt tgataataag gacgacctcg
     1261 gaaagctaag aggctcaaaa tatgttcaga gctatctcgg cgatactttc agaaaagtaa
     1321 aagagctcct tgacgaaaac aagcttgtat tatttaccgg ttgtccgtgc caggtagcag
     1381 gtttatacgg tatgctcggc aaaaagcatt atgacaacct catcacggtg gatcttgtat
     1441 gtcacggtgc accgtcacaa aaatcattca gccgttatat tgaagaaaag tatggtactg
     1501 aaaatcttgc cgactttaaa ttcagaacaa agaaattcgg atacagttca ttcaatcaga
     1561 tcgcctatct gaaggacggc acagaagtac caagcaactt caagtttgac gaatacgaaa
     1621 aagctatgca cagcggcgtt gcgctcaagg acatatgcgg cgactgtatg tttgcttccg
     1681 ctccaagaca gggtgatata tctatcggtg actgctgggg tgtatcacag ttcaacgtca
     1741 attacaacga ccacaaggga acaagcgcta ttcttatcaa taataataag ggcgagaagg
     1801 tctttggtca gataaaagac aggatgaagc tttgcgagcc cataccgtat gatttcctca
     1861 gacatcacaa ccgcttcgga agaaagatga atataccctc gggcagacgc tggttctaca
     1921 cgatgcttga cgggcagtca ttccgcaaat cggtagacta tgcgctgaac agaaaattcg
     1981 atatcggcgt tatcggactg tggtacggaa gaaactacgg aagtatggcg acttactacg
     2041 cccttcacca gacgcttacc aatacgctcc acttatcggt ccttatgata aacaatccgc
     2101 ttacgcctcc ttctgataac tacaacaaga cgcatccaag acttgttgct aagaatttct
     2161 atgatgtgag ccttgtttat ccgctgaatg accttaataa gctcaacgcg cactgcgaca
     2221 cgtttatagt aggctcggat cagctctgga acatttacct cagcagaccg tacagacaga
     2281 tgtactacct tgattttgtc gaggacagga agaaaaagat cgcatacggc acttcttttg
     2341 gcattcctta tgaaggaaca gaggaagaaa agcttatcag ttcaaataat ctcagaaatt
     2401 tcgatcatgt ttcggtaaga gatgatatgt cgtatgatat cgcaacaaac gactttggtg
     2461 tatccgatgt cgaaaaagtc tgcgatccta ctttcctttg tcccattgag gagtatcaaa
     2521 agctcatcga tatggctgaa caaaaagagg acgaaaagta tatc
//
LOCUS       DAWAOG010000288         2562 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5246_length_2562_cov_1.851615, whole genome shotgun sequence.
ACCESSION   DAWAOG010000288 DAWAOG010000000
VERSION     DAWAOG010000288.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5246_length_2562_cov_1.851615"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1171..1222
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          743 a          479 c          458 g          830 t
ORIGIN      
        1 ctttgcaact ccataaaaac ttgaagtaga attacaacag tttttctaat tctatcagta
       61 acttttcttt catatcttct aattctgtct ttgaagggcg gttattatca ttgtacattt
      121 tttccatagg ataccaccaa acaggtcctt ttccgttgtt gccatagttt tctatatctc
      181 cgcttttcct tggaaatagg tgccagtgca gatgtgtatc gccattgcct aacaattcat
      241 agttcatttt ttctgctcca aaagcgtttg aaactgcttc ggcgactatg gacatttcct
      301 ctaaaaactt gatctttttc gaatggttta attgaaacag ttctgttttg tgttctttgc
      361 aaagaaataa tgtgtagccc ttaaaatgct gattatcacc aataactaca taacccgttt
      421 gcaattcttt aacgaagtat ggattcgtac cattgttgat cattttaatt ctgtcacata
      481 taaagcacat actctaaacc tctcatttca tgtttgtccg gaataataac tacttgttca
      541 agactgttat agcataatcc tttatgtcag tctgctttat aaatgaaaaa aattataaaa
      601 tcttttccat atacccacag gtaaaaggga aaaaatcata ttttttggtt cctgtatgtg
      661 taaatccgaa ttgctcatac caatttttta attttttgtt ttcctcgact attccgatgt
      721 tgatttttac gcaatttgtt tttttggcag tattaaaggc atgtaataac agctcttgac
      781 cgatttttct gtgtctatat gccggaagta cacacaaatt gcttaactca cactcattat
      841 tactctgcaa tagcaatgaa tagtagccaa caattttccc gttatcccaa aaggcataca
      901 tacgtctatg ctcaccgaat aaatgccact ttaatctttc ttccgtcgtt gcgaatgctg
      961 tgaatttagg tgcattttca gcggtaaagc caaactcgtc agcaactgtg gcaaagctga
     1021 ctctgattac atttacacat tcgcaaatat ctttttcatt cacttcctct atcatggtaa
     1081 gtacctccga atttcgattt aggagcagtg cgttggttct taaataaatt gtatcatata
     1141 aaacttagta attcaaggga tatttgcaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nntctatgct acaacgtaat cacaacgata aaaggagcaa
     1261 gaaaatatat ttaaacctac tgaagaaaga ccttaaaagc aaaaaaaact atgaatatga
     1321 tactgctggt gtttattata gttgcgacga tgtttgtatc ttcaagcgta aacaacattt
     1381 taagggttac aacggcgctg aatgattatt ttgaaatggc gaatgcacct gattatctgg
     1441 tgacaacaat gaacaaaaac cttgccgttg acattgatga aacaataagc tcagcgaaag
     1501 cggttgacag ctatgctgtg gaagatgtgc tgtttttagc acctgacaat tttatcttcg
     1561 gggacaagga cataaccgca aaaggcggca cgaatatagt tcagagcgac ataggtatga
     1621 attattttct ctctgacggc agtatccttg aaaaagtaaa tcagggcgaa ttatatataa
     1681 cggaaaataa agcagccgct atcggcgttg aaatcggaga taagcttacc gtagaattga
     1741 acggcatcaa ctgtgagtta atattcaaag gcaagataaa ggacgcactt ttcggatcta
     1801 atcagcttac tataacccgt tatataatca gtgttgagga ttataatgca ctgacttcag
     1861 ctgaaaacgc agaggagttt tacggtggct ctcttgtcta catacattcc tctgatattg
     1921 atacacttgt aaagcagata aagccccttg cggacaacag cattcttaca tttgacaggg
     1981 cttctatgga attctgttat atttttgata tgataattgt ggggctgctc attgtggtaa
     2041 gtgttatttt tattgctgtc gctttcgtgg tgctgcgttt tactatcacc ttcacgcttt
     2101 cggatgaatt tcgtgaaatc ggcgttatga aagcaatagg tataggcaat ttcaaaatcc
     2161 gtgggcttta ccttgtaaag tatctggggc tttcagtaat cggtgcggta atcggacttg
     2221 tattaagctt cccattcggt gaaatgctta tgagcgtatc atcgcagaat attatcgtaa
     2281 gtaattcaaa tccgatattt gtaaatattc tctgcgcagt gcttgtgatt gcggtaattc
     2341 tgtcgttctg ctatggctgt acgggtaagg tcagaaaaat gactccgatt gacgcaatcc
     2401 gaaacggtca gacaggagaa cgtttccgaa aaaagagtgt aatgagcctc gctgaatcaa
     2461 aactccctgc aacacctttc cttgctctta atgatattgt aagcagccca aagcgttaca
     2521 gtattattac attcacattc ttcctatgcc tttcccttat gc
//
LOCUS       DAWAOG010000289         2559 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5253_length_2559_cov_2.216054, whole genome shotgun sequence.
ACCESSION   DAWAOG010000289 DAWAOG010000000
VERSION     DAWAOG010000289.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5253_length_2559_cov_2.216054"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          775 a          570 c          569 g          645 t
ORIGIN      
        1 catctgccgg gaatatcaag aagctgcata cccgatgcat cggaaacttc ggtagcaaac
       61 tcgcccgtca gcataaatct tatatagtcc ttagggagca gaatgtgacg gcacttttcg
      121 taattttcag gctcgttgtt ctttacccac atgatctttg cggctgtaaa gcctgtgata
      181 gcggggttag ctgtgatagc gataagtctg tcatgaccta ccttttcggt tatctcgaca
      241 acttccttac ctgtacgctg atcgcaccag ataatactct ttcttattac cttgttatcc
      301 ttatcaagca taacaagacc gtgcatctgt cccgaaagac ctacgccctt tatctcatct
      361 gcggggactc cgctctttgc aactacgtca cggataccgt ttacgcaggc attccaccag
      421 tctgtgggct cctgctcggc atagccgttg cagggagtgt aaagaggata atcgtaaagc
      481 gctgatgcaa caggtgtgcc gtcttcactg aaaagtacag tctttgtacc ggacgttcct
      541 atatcgacac cgagtatgta tgccatatct gttctcctta tttttcaaaa cttcatagaa
      601 ttttgtcgtg taaaacctta cacgcttctt acataattat aatctatcgg gcttacgttt
      661 gtcaatagaa aaaaccgaaa tatcgtcatt aaaaacgcca ccgccgcccg ttgaactcag
      721 cggtcggcaa aagccgttgc atgatgccga cgaaatggtt acaatcttgt aactttcatt
      781 gactttacca aaatgtgcta ttataataaa gtcagaaatt gttgtccgtc tgtggacaat
      841 caacagctct gcggtgtatt atatacagaa gagcaaatcg cctttgcgaa aggagaacag
      901 tatgaaaaga agaatttcat cagcgatcat cacaaccgca ctgaccttat cggcactcac
      961 cctctcggct tgtcagtcgg aaaaatccct cgaaacaacg gatcttatga caggcatcaa
     1021 agccgcaagc cacgaaaccg taaaacccga cgatgctttc aaaaacgcat acggcaattt
     1081 ctctgtcgaa ctgcttaaga aatgctttga cggcaaatca aacactctta tctctcccct
     1141 gtcggtatcg accgcactca caatgactgc gaacggtgct aacggtcaga ccaaagacga
     1201 aatggaaaag gtactcggga gcggaatgcc ccttgacaag ctgaacaagt atctttcatc
     1261 attcagcggc tcgcttacat caggcgagga cttcaagctg aaaaacgcaa attcaatatg
     1321 gttcagagat gaagaaaaca gacttaccgt tgaaaaggac tttttacaga caaacgccga
     1381 ttatttcggt gcggcgatac acaagcgtgc gtttgacaac gatacttgca aagagatcaa
     1441 cagttgggta aacgacaata ccgacggtat gataaacaat atacttgaca gcattccgga
     1501 cgaagcaatt atgtatcttg taaatgctgt cagctttgac gcggaatggg atatcatcta
     1561 tcgcgaaaat gatatagcgg acggaaaatt cacaaactcc gaagacaagc ttatgaaagt
     1621 cactatgttg tgctcagatg aaagtattta tctggaagat gaggattgca agggctttat
     1681 aaagcagtac aaggacggca agtacagctt tgccgcaata ctgcctggcg gtgatatcaa
     1741 aagctttata acatcgttat cgggtgaaaa attaagcaag atactcggaa gtgccgaaga
     1801 ctgcattgta gaggtaaagc ttccgaaatt tggctatgaa tattcctcaa agctgaacga
     1861 tgccctttcg gcactcggta tgccgactgc gtttgaccaa aataacgccg atttttcagg
     1921 tatcggtaaa tcgacaatgg gcaatatatc catcggcgaa gtgatccaca agacaaaaat
     1981 agaggtaaac gaaaaaggta caaaggcagg agccgccgct cttgtggaaa tggttgacga
     2041 ggggtgtgta attgccgaaa agcaggtaat tcttaatcgt ccgttcatgt atatgatact
     2101 tgacaatgaa acgatgctgc cgctgttcgc gggcatatat acaggcgtat aacgcacaga
     2161 aattgtataa tctgcataat ccgagctata atattttctt gacttttgga gtatgtatgc
     2221 tataatttaa ttagcagaaa acaacaaatc tttcaggaaa ggctggatgt acaaatggca
     2281 aaacgcagaa taggtatact gacaagcggc ggcgactgcc ccggtctcaa cgctactata
     2341 agaggtgcgg cgaaagcctg ttacactatg ttcggcgagg acaacgttga gatagtcggc
     2401 atatccaacg gttttcacgg tctgatccat aataactgcc gcataatgaa gccggaggat
     2461 ttttccggca tactcactca gggcggaacg atactcggca caaagcgtac accgtataag
     2521 atgatgcagg taatagaggc ggataacata gacaaggtc
//
LOCUS       DAWAOG010000290         2543 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5303_length_2543_cov_2.456592, whole genome shotgun sequence.
ACCESSION   DAWAOG010000290 DAWAOG010000000
VERSION     DAWAOG010000290.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5303_length_2543_cov_2.456592"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          776 a          451 c          611 g          705 t
ORIGIN      
        1 agatattctc ctgctacaag cagaaggctg aggtagaggt aacagatgcc atacccgatg
       61 ccgaaaagct gaaataaaca cggtcggata tgcaccgcag tgggaacact gcggtgtttt
      121 tgcgtttacg gttgcactgt cgtggcagtt atggtaaaat gtaagtacaa aataatacgg
      181 gaggcagtta tgaataatat tgataagctc acgcaaaaaa tattcagcaa attcaatgac
      241 aattctttgt ttttcagcaa tatatatctg accggaacgg aagaaaacaa agctgttgtt
      301 ctctttgata tggaaggttt cattttaaag gtttgccttg acgaacttaa aaccgaatat
      361 acaatgcctg aagacagcta tgtacttgtt tctgagatgt gcattgatga aaatgaaaat
      421 gtgattcatt tctcagtatg gagcgaagaa cgaggtgatg aagattttga actgaagttt
      481 gatcaggcaa atgctgaaat gatgccatgc aggaaaacat attactccga tggcgtatgg
      541 gatatagtgg tctgtatggc ggcagatata tacggcagat actgttttga tgaaactttt
      601 atcagcgaag cagagcgtaa ttatctaccg cttgtgtttg aacttatgaa aattacagac
      661 ggttctaaag caaaacctga attaccggtt cttacagctt atgccgaaaa gtacggtctg
      721 aatgagttta ccgcaattat cttaaagaat gccagacgtg ataaaacagg aatttacaat
      781 aaacgtttca gcgggcttga tgatgttaaa tatgaaccgc tctggcgtga actgtatatg
      841 attttttggg ggatttgcaa ggagtatccc actgtatcgg aaataatcgg acttgaaccc
      901 gaaaacattc ggataagaaa aaatatcacc gacacgcttc ataaagcagg ttatgagggc
      961 gtttatcccg atttcagaaa aacgggtgaa cttaaaggcg tacatcttac ccaaagtcat
     1021 gataaggcgt atttggtcgg ttgtgagaag aatgtccttt atatggtaca ctgcgatgaa
     1081 atgtgctccg acggagagcc ggtaatcata ttcaggagcg gaactattgt gatgaaagac
     1141 ggctttgact atagtaatgc cgatatttat tcaagtatgt tccgcaatgg tggttatcat
     1201 atagaaaaaa gcttttcgtg ctgtgcagga aacgaggata tttcacaagc cgccgaaata
     1261 gccgtaaaga gagcagaact gaaaaagctt acacgaaagg aatgtaaagc ggctgacgtt
     1321 gataagaatc ttttgtcatt ccttcctgtc ggaatgctga tgggtctgtt attcggcgtt
     1381 ttcttggcgc tcggtatgat gattgttttg ttcctgtttg aaatgtttgt cgggagcaat
     1441 gccgttgagg cgttacaagc gatagttgac agtaggtggc tctgtgcgtt cggagcatcc
     1501 ggacttgcgt tcgggcttgc aatgacagtt ttaatgtatt tggcaggaag aaaataagag
     1561 gtgcaaatta tgcttactat gagaaaaggc ttttcaattt ccttcgccga taagctcagc
     1621 gaaggttata aaacggacgg aaaattcttc accgcaaacg tatcggcaca aaaaactctg
     1681 cccttgcttg aaaatttcat aaaactgcac gaagatgaaa agctgttctt tattctggag
     1741 ctgccgactc ccgaaaccgc cgagccgaaa gacgaaaacg gaaatataac agcgtttcat
     1801 aaggatatct attatcttga tgactgtacg ccggcgatgg taagagatgt gcttgatatc
     1861 gtgggggaaa tgctacttga cgacggaata tcgcagttcg gtgtgggtgc gcattcatcg
     1921 tgcgatgaga tgatgatctg caagtacaat gtcgcttgcc tgtatcgcaa tgatgagaaa
     1981 agcgggattt atgacggctt ttttgaaaaa tatggcataa ataagaccga taaccttgtt
     2041 accgcatggg atatgctctc tcccggaacg cccggcgaat gtacaatgtg tgaaaaggac
     2101 ggaagaaccg tttatgatat cccgtccgta tttgccgagc ttgggatgta tatggcgaaa
     2161 aggtgcgagg ggtaaggata ttcaccacat tcgacctcag acatataatg ttaaaaaagc
     2221 ggcagaatgt acggtggctc tgcccgtgaa ttgccgtgca tggctttacc tgccgctttt
     2281 ttgactgaaa aatgctgtac aggcagtcat tttcggctaa atattgcgag agaactcggc
     2341 acatattgca gaaaatcacg ccgttttttc aaataaacgc tgtttttgca tattgaacat
     2401 tgcaaaatgc agtttgacgg caaatatcat tcatacgtca aaattgcgca taacaccgca
     2461 aatttgatga tatctgacga ttttaagaag atttgagcgt tataaatacc acttaagcct
     2521 taaatgagat agcatatatg cat
//
LOCUS       DAWAOG010000291         2533 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5325_length_2533_cov_2.494350, whole genome shotgun sequence.
ACCESSION   DAWAOG010000291 DAWAOG010000000
VERSION     DAWAOG010000291.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5325_length_2533_cov_2.494350"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1486..1495
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1884..1893
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          762 a          503 c          613 g          635 t
ORIGIN      
        1 ataatgtata tgtacgcagc aagtttgaag ccgaagttga ggtacttgag gctatccgaa
       61 acggacttaa tgcgaagata ataagagtag gaaacctgac gaaccgtctt tctgacagca
      121 ggttccagcc caactataag tccaacgcat tccttacaag attcaaggcg gcgctggatc
      181 taggcatggt tccggattat ctgctaccga tatatgcgga attctctccc attgaccaga
      241 ccgcagacgg agtggtaagg atagcgcagt acgctgatga tcagtgcgtt ttccacctca
      301 acagcaaccg cccgatctac tttgacaggc ttgtagaggt gctgaatcag cttggatacc
      361 gaatggatgt agtaagcggt gagaaattca accgtactct gcaaaatctg gcaaagaact
      421 caaaaaccga atacatctac gaagctttcc agaatgatat gaacgagaac ggagagctgg
      481 tttacgacag caatatccgt atcaggaacg atttcaccga ctggtttatg aaaaaagtcg
      541 ggtttgaatg ggcacagata gactttgatt atatcaaggg atatctggaa tacttcagga
      601 acttaggata ttttgaggtg cgcaatgaag aataaaccgt atccttatta caccaatgaa
      661 gaataccatg atttgaagca gatggtgtat tccaaggctg agaaaatacc ggataaaccg
      721 gcgttcagct tttcagccgc aaagaatacc gttgtcacta aaactttcaa agatttttgc
      781 gatgacgtcg aggctttcgg tacttatttg cttgcaaacg gatacagcgg tcacacagga
      841 attataggcg aaaactcgta tgaatggctt gtcgcctttt tcgctattgt gaatatgggc
      901 gcagttgccg cgcctatcgc taaagatctg tcggcagacg aaatagctca actgattgag
      961 agggctgact gcaatgcagt tcttgtatcc aaaagctata cggaccttgt gagtgtgcag
     1021 gacggcatta agctactcag cctcaatgat attcccacgt atatcaacag tggcttggag
     1081 cgtattcaaa gtggggactc cagcttcata aacagtcgaa tagacgccga caaaatggct
     1141 gcgatcttct tcacctcggg aacgacaggt tcaagcaagg gagtaatgct gagccataag
     1201 aacatgatct cagacacaat attcgcctgc cgtaatttca gactgacagg taacagtata
     1261 gctgttctgc cgtttaatca cacattcggt ttgataacag cgattatggc tcaattcaat
     1321 tatgaattct gctcgtttat aaataaaagc acaaagcgtg tttcgcagga tctcctgact
     1381 gcaaagcccg taggtacatt tatggttccg ttgttcatcg agacctttta tcgaaccatc
     1441 atatccacgg ccgagaaaac gggcaagctg aaatagctgc aaacannnnn nnnnnaaaat
     1501 cagcaatgct cttctgaacg tcggaataga cctgcgaaag cagctgttca aaagcgtgcg
     1561 caatgccttt ggcggtaata tggagtacat aatctgcggc ggtgcgccgc tggaagaaaa
     1621 gtatgtaaag aagttccgtt cgtggggcgt taatctgtac aatggatacg gaatcacaga
     1681 atgctctcct gtcgtatcgg taaatagaaa ttcatactgg cgtgacggga gcgttggacc
     1741 ggttctcaaa ggctgcgaag taagaattgc ggctgacggc gaggtactgg ttaagggcga
     1801 taatgtaatg ctcggatatt acaatgacga acagtccacg gcagaagctc tggtggacgg
     1861 atggtatcac acgggtgatc ttgnnnnnnn nnnatcttgg gtatattgac acagatggat
     1921 tcttatttct taccgggcga aagaagaacc tgataatact cagcaacggc gagaatgttt
     1981 caccggagga gatcgaagct aggatattga atgacgacgc tgtttccgaa gtggttgttt
     2041 acgatgacaa aggtcagatc gttgctgaaa tataccctgc cgatggattc cttgatgaca
     2101 aaccgcactt tgattcgctc ataaggcaaa tcaacgacgg tcagcccaaa tacaagcagg
     2161 tcagtgtggt caagctcaga aagagcgaat tccagaagaa tgcaactaag aagattatac
     2221 gctataaggt gaaggaggaa caaaataatg tttgataagg ttgtgcagat acttggcgat
     2281 tacacggatt ttccaataga aaaaataaat gaatccaccg atttggtaaa tgaactcggt
     2341 cttaattctc ttgatgtaat gaatgttgtt gttgcgtttg aggatgagtt tgggattgaa
     2401 ataccggata gagctatcag tggcttccat acggttggtg atattgcgga ttatcttgta
     2461 aagaaactga attaaaactc aaaacgaatc agatgttgca aaactgaagc aactgtgcaa
     2521 aacttccgta ccc
//
LOCUS       DAWAOG010000292         2530 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5335_length_2530_cov_2.599192, whole genome shotgun sequence.
ACCESSION   DAWAOG010000292 DAWAOG010000000
VERSION     DAWAOG010000292.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5335_length_2530_cov_2.599192"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          655 a          511 c          670 g          694 t
ORIGIN      
        1 gtgcaaccat ttggagctaa ttttcttata agcacaaaaa gttgtcttgt ttttgttttg
       61 acattatgtt atactttttt atagtcgtcg gaattgcgac atatttatta agcggcatgg
      121 agaggtcggt atatggatat attgaaaacg aaaaatgata taattaacgg aaaaacagcg
      181 cttggaattg agttcgggtc tacaagaatt aaggctgtcc ttataggcag tgataactcg
      241 cctgcggcaa caggttcgca tgagtgggaa aacagacttg aaaacggtat ctggacatac
      301 agcgaaaatg atattatagc aggtatgcag gattgctatg cgtcgttggt aaaggatatc
      361 gagtcgaagt acggcatagt gccgacgatg tacggctctg tcggaataag cgctatgatg
      421 cacggatata tggcgtttga taaagccgac agattgctga cacctttcag gacatggcgt
      481 aatactatga cggagcaggc aagtgatgag ctgacggagc ttttcggctt tcctgtgccg
      541 cagagatgga gtgttgcgca tctgtatcag tgcatactgg caggagaaga gcaccttgta
      601 catctggaac atctgactac tcttgcggga tatattcatt atcttctgac tggtaagcgc
      661 gtgctcggaa taggcgaagc atcgggtatg ttccctgtag attcagccgc gcttacatat
      721 aatagtcaga tggcggagaa atttgataac gccgcacgtt tacacggctt taataagcct
      781 gttttatcat tgctccctga agtgctcacc gcgggaaata atgcgggaac gcttaccgaa
      841 agcggagcaa agctgcttga tgtgtcgggc aatcttaagg cggggatacc cttctgcccg
      901 cccgagggcg atgcgggaac aggtatggtg gctacaaaca gtgtagcacc gagaacaggc
      961 aatgtttctg cgggaacatc ggtatttgct atggtggttc tggaaaagcc actcgcaaag
     1021 cttcataagc agatagatat ggtaactaca ccatcgggtg aagcggtcgc tatggtgcac
     1081 tgcaataact gcacctcaga gctgaacgca tgggcaggtc tgtttaagga atttgccgcc
     1141 gcggcaggct ttgatattga cagcggcgct ctttatgagc tgctgttcaa aaaggcactt
     1201 gagggcaaaa aggactgtga cggcatgata gcctataact atctttccgg tgagcacgtt
     1261 acgggatttg aacagggaag accgctattt acaaggcgac ccgatgccag cctcggattg
     1321 gctgacttta tgcgtactca gctgtatacc tcgctcggtg cactcaagac aggacttgat
     1381 atacttacta aggaagagtg cgtgcctata gacagtgtaa ccggtcacgg aggcttcttc
     1441 aagacggaaa aggtcggaca gtcgatactt gccgccgctg taaatgctcc tgttaccgtt
     1501 atgagcaccg ccggagaggg cggcgcatgg ggtatggcac tgcttgcggc atatatgtcc
     1561 gacagatcgg ataaaacgct tgataaatgg ctttctgaca atgtattctc atctgcacag
     1621 acaaacgttg tagcgcccga tcctgatgat gtcaaaggat ttgataagtt tatggaaggc
     1681 tataccgagt gccttgccgt tgaacgctcg gcggtcgatc atctcggttg aaatgcacta
     1741 ttgactttat gtcaagccgg gtgtataata taaatgtatc tgcgtgatac gcagataatt
     1801 acagaggtat agaatatatc aggaggaaat tgatatgcca ttttgtccca aatgcggatc
     1861 tgaatatcag gatggagtca agttctgcgc aaagtgcggc ggaaatcttg acggttcggt
     1921 agcgcctgtt cctgtaaatc agggtcccgg tttttttcag aaaatacttg atacaaagga
     1981 tataactgct actttcgatc ctgcggatat aaacgcagga aaagcaatgt caatactcgc
     2041 gtattgcgcg ttgctcgcat acatacttgt aggctggatc ttcggcggct ttcttgcgct
     2101 catcgtatgt gcgggaatgc ttgttgctcc ttgtataatc gcaaagaaat ccaagttctt
     2161 gcagtatcac ctttcgatga tcttccccgc gcttctcggt gtaatggtgg tgaatgtggt
     2221 cgaaggcttt ttatcggcaa agctttactg gtttgtttac agtacgatac ttaccggtac
     2281 atggaatgag tttgcggccg gatttgtagc tgttatcctg gcatggttcg tacatatcat
     2341 atttatggct gtgcctgtgc tgatactgat agcaggtctt gtaaatgctg tcaagggtaa
     2401 ggctaaggat cttccgctcg taggcggatt caagtttact tttacaaagt aattttaaca
     2461 tcaatacgca gacagccccc gattttcggg ggctgttttt tatatttccg gcagtggcat
     2521 aatatgggaa
//
LOCUS       DAWAOG010000293         2529 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5338_length_2529_cov_2.183913, whole genome shotgun sequence.
ACCESSION   DAWAOG010000293 DAWAOG010000000
VERSION     DAWAOG010000293.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5338_length_2529_cov_2.183913"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2066..2075
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          709 a          562 c          410 g          838 t
ORIGIN      
        1 ctgccactga agctaatatc aatctgcttg ctcgtatcat ttcagctgaa ggcagaggtg
       61 agccttatat aggtcaggtt gcaattggtg ctgttattat gaaccgtata gagcacccct
      121 ctttcccgga tactctggca ggaattatat atgaaaacgg tgcatttact gctcttgtgg
      181 acggtcagtt caatgaacct attgccgact cagcatatga tgccgcccgt gacgcactgt
      241 caggctggga tcctaccggt ggctgtatat actactataa ccccaagaaa acaagcaacc
      301 aattcatgat gagcagacct gttcaaaaag taatcggtga ccattatttc tgcacataat
      361 agaaaagaga agcgtctgct tctcttttac atattgttta ttttaattgg attttactgt
      421 ttgtttataa atttgcctcg cccacagtat tgtccactct tctgagtgtc attatcataa
      481 gtgccagtaa acagcatatt acggatagaa taaatgctac tactgtactt aatataaaat
      541 tgttcatatc tgcatcatta tatgaactca tcagcaatga ctgaagtgta aacggattat
      601 aacgatctat atcgagcgac tgcaacaata tcggtataat tgtcgaccca ccgtaaacaa
      661 ttattacaga tattccggga cgacctgttg acagcccgat aagaaaatac agtgcgatca
      721 taaacaacat ataaattgcc gcacatagcg atgaaaacat aatgtcattt atacttaact
      781 cattgttgta catcaggtta cttatacagc ctgtaattat cccgccgagg aacgataata
      841 ctcctatcac taaaggatac actataaact taggcagaac atatccggca ggagtaagtc
      901 ccgcgcagtt aggtattata actgaacgtt tcttctgttc cccgcctgcc gttgccatga
      961 gcagcagaga tattatcaaa aatcctgttg aagtaattga gctaactccg gttttaaatc
     1021 cgacagccgc catacttccg ccgtacatag cttccagtga gctcatcatg ccgttgattg
     1081 aatctaccgc acctgttacc tgatcctgtg cctctcctcc tatctgctgt cccatatccg
     1141 ctatatgccc tgccatcatt tccattgact tgtacaacag cgggtacatc agagaaattc
     1201 cgattatagt gataataacc ccaatcagtc tgaatccacg gctaaatagc ataaattctt
     1261 tttttattcc ggccttaatc atatctgctc ctttctcaac ctattgtatc catatatatt
     1321 tgttcgagag tcttgttttc tataacaaaa agtgataatg tcaaccccgc agaatttatt
     1381 tcgtttatta tctgttgtgc ttttatctgt tcgttttcat catattcaag aggaatttca
     1441 aatattgctc tttgtctgtc aaagtaaaac ctcccaccgg tacacttatt caccgactga
     1501 atagctgaat ttggatcgcc gaatattccc ataatgattt tcttgtcgtt tctggcaaat
     1561 tgatccttta tttctgccat agtcccctca acagccattc ttccatccct tactatgcct
     1621 attctgtttg cagttctttc aacatcagag agaatatgtg tacaaagaat aatggttgtc
     1681 cccatatctg caagctgctt tattatattc attacttctg ctctgccttg aggatcaagt
     1741 gccgatgtag gctcatcaag tattagcaag tcaggattat caaagatagc cgctgctatg
     1801 ccgatacgct gattcatacc tctggaatat cctttgattc ttctttttcc gccctcagcc
     1861 ataccgacaa gatttattac ctcatctatt ctctttttct tgtcgccttt atatctacag
     1921 cacgccgcaa tataatcaag atactcatat ccgttcatat atgtaaacat agacggtgtt
     1981 tcaggtaagt acccaacttt acagttttcc ttgccgaata taatttcgcc gctgtccttt
     2041 ggtattatat tagaaacaat attcannnnn nnnnnggttg tggtctttcc acagccgttt
     2101 ttaccgagaa aaccatatat atcgcctttg ttaacgttca tatcaagtcc cttaagaact
     2161 tcaaaagcac cgtaactttt atgtagattt tttatatgga gcatttctta caccctttca
     2221 ttgcattttg taaaaaagta aatactattt acctttgaaa ttgattatat catatcagct
     2281 aaaatttgtc aaacaaattt tacttgagcc tcaaagctta cttttccgtc atcaaatctt
     2341 ccctcaactg tacaagtatt ttctttgatt tcaagataca gactgatagt ttcgtcttgt
     2401 ctgcattcat gtataaagtt tattctgact gtatcatagt gcttttcgat aagttcttgt
     2461 ggaattatgt cactcataag tcttagatat acgccgttgt ttacatgacc attgggatca
     2521 atatcactg
//
LOCUS       DAWAOG010000294         2526 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5350_length_2526_cov_2.374747, whole genome shotgun sequence.
ACCESSION   DAWAOG010000294 DAWAOG010000000
VERSION     DAWAOG010000294.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5350_length_2526_cov_2.374747"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          759 a          510 c          659 g          598 t
ORIGIN      
        1 attttatgaa tttgcatttt tcgcagttca tggataaagg ctggagcttc atatttgacg
       61 cttgctacag cgatgcaaag gcgggcggtg acggtcatgc actggtcgat gcaaagtaca
      121 gctatataac cgcttgcagt aatgacggcg actacagcac gataattaca aacacgacat
      181 cagagcctat aacttataac tttgaggtaa caaacctcgc aaaagcggat aatgaagtat
      241 atgtatggga aacaagaggt cctgatgagg gtcgggagta caacgcaaat tactttaaga
      301 agatctcgac agttacaccc gaaaacggca agtattcggt tacaataaag ccgtattcga
      361 tgataactgt atcaactttg aatatatcag agcctgaatt tgatgtgccg caggaaagca
      421 gcaatacgtt actttcgttg ccgtacacag atgatttcgg ttattccgat gagtttcttg
      481 cttcaagagg aaacgctccg ctttatacta ccgatgaggg cggcgcattt gaggttgccg
      541 acaaggacgg caaaaaggtg ctcatgcaga agataaccgc agatatcaaa gccgaagagt
      601 ggggcggaac gcctaacccg actacaaact tcggtgacga ccgctggtat aactacagcg
      661 taagcgccga cataatgaca gacggcgagg gaagctatgc gggtgtcgga ctcagataca
      721 gtcttgccga cagcggcaag agcggctaca gcgttacgct caacgaaagc ggcacatgga
      781 tactgttcgg cgacaagcgt aagatcgata ccggcgagat aagcggtttt gacgcgacaa
      841 aatggcacaa tataaagatc tcggcactgt ataaagctat aacagtgtct gttgatgata
      901 tcaagataac tgagttcaag gcggaaaacg cgggctttgg ttcgggaaga gcggcgctgt
      961 acagctcgta caacaactgc ctctttgaca atgtagaggt tgaagctacc gatacggttc
     1021 agccctatgt caataagtac gacaatctcg atgcgatatt cacatacagc gcggacggct
     1081 gggagcacag caccatggac agcttcaaga acttcagacg tacgctctcg cacggaactg
     1141 agggtgcgtc cctgactgtg gattttgagg gcacgggaat aatactcaca ggtatgcaga
     1201 gcggcgaagg aacgatcaac gtatccgttg atgataaaag catcaaagag ggctatgagc
     1261 tgtctaagac cgcaaacaga caggcattca ttgtgataaa cggacttgag cagggcaagc
     1321 acacgctcaa gctcgcagta accggcggca agtgctcgat agacgcggca caggtgcttt
     1381 acgattatgc ggcggtaaag ccgacaacag aaaaaactgt ttcatcgtct gccgaagaag
     1441 taaattcttc caaagttgaa agctcagagc ttgccgaaag cagtaagacc gataacagct
     1501 catcgtctga cagcgcagag agcacaagtg ccgacaacgg cgaaaattcg ttccctgtag
     1561 ttcctgtagc ggtgggagcg gctgttgcgg ctgttgcggt cggtgtaggc gttgcgatag
     1621 caaaaatgaa aaagaagaaa gattgataat caggagggta tattatgtca agatttgaga
     1681 taagagataa gttttatttg gacggaaaac cgttcaaggt aatttccggt tcaatacatt
     1741 atttccgcac tgtaccggaa tattggcagg acaggcttga aaagctcgta aatatcggat
     1801 gcaatacagt tgaaacgtat attccgtgga attttcacga aactgaaaaa ggaaatttca
     1861 attggaacgg aatgcatgat atctgccgtt ttatagagct tgccgataaa ctcggacttt
     1921 atatgattat cagaccgtcg ccgtatatct gttcagagtg ggaattcggc ggattgcctg
     1981 cttggttgtt aaaagaccgt gcaatgcgcc tgagatgctc ctacaagccg taccttaatg
     2041 cagtggaaag ttattacagc gttcttatgc ccaagcttgc accttatcag atcgataatg
     2101 gcggcaatat aattatgatg cagatcgaaa atgagcatgg ctattatgga aatgacacgt
     2161 catatcttga gtttttgaga gatactatgc gtaagtatgg tataacggta ccgtttgtga
     2221 catctgacgg accgtggagt gaatttgttt tcaaatcagg tatggttgac ggtgcattgc
     2281 cgaccggcaa tttcggctct tctgctgaaa tgcggcgttt tatcggagaa gataagccgc
     2341 tcatgtgtat ggaattctgg aacggctggt ttgacgtctg gggagaagaa cacaatatca
     2401 cagcacctga aaaggcagct caagaacttg atactctgct caaaaagggc agtatgaatt
     2461 tctatatgtt cgagggcgga acaaatttcg ggtttatgtc cggaaagaac aatgagaaaa
     2521 aaacag
//
LOCUS       DAWAOG010000295         2526 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5353_length_2526_cov_2.091461, whole genome shotgun sequence.
ACCESSION   DAWAOG010000295 DAWAOG010000000
VERSION     DAWAOG010000295.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5353_length_2526_cov_2.091461"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    888..897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          875 a          473 c          518 g          650 t
ORIGIN      
        1 ataaatcctg aaaaactgac gctcggaata atgggttgcg ctaatgatat gcaaggctca
       61 catcgtattc gtttgcaccc aaagcattgc tatgagcttt acagcaaatc gcttgttatg
      121 gcgatgaaaa aggctttctc ctattgggac ggagaaaaaa gctatcgtct gacaggcgat
      181 tatataaaca ctgaaggaat tgcttgtttt aatttatccg agtcagttcc gataaaaccg
      241 gatgaggttg aattgtaatt atggaagatc gcaaaaatcc taaattacgc tgtaattcgg
      301 aatacagacg attataccag ccgatgacag tacaggagta tgaaaaactt gagtctgaac
      361 tcaagaacag caaaaataca acagtagaaa taaaaacatg ggtaaatatt gttttatttg
      421 attacgaaaa acttgaaatc tgcaaaaaac acaatattcc ccatgtagac tcacgcttat
      481 atgtccgcaa cagcgaggaa gcgttgcttt ggctttgcaa aaaacaattg gaacgtgagg
      541 atttagcatt ggaaatgcgg aaatatctta tcgggaaacg ctacctttac gaaaaaatac
      601 tcggtgcaca tacaatgtcg gcacgacgtg ccgcaagcag ctcaaaggaa agccataaaa
      661 tgaacgagcc gcgctacgaa tattgcgcaa tgaaaacgag agagcgttta gcgaaggaat
      721 ataatgtggt tcatatgacg atatggaaat acagcatatt cgccgaagca ctggacacaa
      781 tttacgatat ttccgaagag cttgctactg ctattatgcg tggtaaccta aaaatatctc
      841 aggaaaatgt agtagcaatt tcaaaattat ccgaaaggga aatactcnnn nnnnnnnact
      901 caatatggct gaacaatttt tgcggaaaca aactgatcgc aagagccttt cagatccacg
      961 aaagctctta tcaaaagcaa ataccgaaaa gtcagatgac accgtttcgg cgaagtcgcc
     1021 atctataaaa gatatgccgc aatatgaccc tgactcggag gcagcgagcc ttacgcttac
     1081 tattccgtcg tggataagtt ctatgaaccg tatcagcaac gccggcgata tgagcaaatt
     1141 cagcaaaggg gcaaaaaaca agctccgaaa cgaacttgat aaactcggcg atgctatatc
     1201 ggatatgtat gatatgttga aggaggaaaa aatataatgg aagaagcgga ttatagcaaa
     1261 tttgttccca atgtacattt tgagcagata ccaataaaga accttgttgc aaatcaggaa
     1321 tatcagcgta atttatcgca aaatcacatt gtgagagcgg caaaaaatct tgacctgtat
     1381 cagataaatc ccgtgaaagt cagcaggaga aacggcataa attatgtatt taacggacag
     1441 cacaccattg aaatagttgc tatggtatcg gagtccagag atacgcccgt gtggtgtatg
     1501 gtttatgatg atatggatta tagcgttgaa gcggacgttt tcgccaatca acagaaatat
     1561 gtaaaagcgc tggcgccgta tgaaatatac aaagcaaata ttgaagcagg caatgataaa
     1621 caaattatga taaaatcatt agtggagtca tacggcttaa caataggacg cacaaaggga
     1681 caaggggtta tatgtgcagt atcatctttg gaatatattt tcgatacttg gggatttcac
     1741 gttcttgacc gtgctttgcg attatgtata ggcacttggg agggtgcggc aaattcgctt
     1801 tcttcaaata tgctgaaagg tattgcacga cttattgtgg catttgacga gaaaatgcgc
     1861 gatgatatat tcaaggaaaa ggtcggagct tattccgcaa aggatattat ccgcacggca
     1921 aaggaacgtc gtgcaggtgc aataggatat gccgaggcaa tgcttattgc gtacaacaag
     1981 aaaatgaaat atcctctcag atggagcaat ctttacagca atcccaaagt caaaaaagct
     2041 gtagaagcta ttgaaatgga tattatgagt acggatgaag aagcggacga tatgctgaca
     2101 tttttttgat ggcgaaacag aataagaaaa ttttcgttag tcatatgtat ttattattca
     2161 tctcactata ctctcaaaca atggggggaa ttatcaggca agcacaccag ttttactacc
     2221 ctataaattt acactactct caaaccgcag aagctatgaa agtgttgtgt cgatagtttt
     2281 actaccctat aaatttacac tactctcaaa caaactctta ccataagttc aagcgtcctg
     2341 cgttttacta ccctataaat ttacactact ctcaaacggc tgtccgttag cgagctataa
     2401 aaaacgagtt ttactaccct ataaatttac actactctca aaccaatgaa aaaagtcagt
     2461 attacgacac acagttttac taccctataa atttacacta ctctcaaaca agctatacaa
     2521 cagact
//
LOCUS       DAWAOG010000296         2523 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5361_length_2523_cov_2.066045, whole genome shotgun sequence.
ACCESSION   DAWAOG010000296 DAWAOG010000000
VERSION     DAWAOG010000296.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5361_length_2523_cov_2.066045"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          700 a          551 c          668 g          604 t
ORIGIN      
        1 acaacatcat tcacgacaaa ctatgacgag aaaacagttt cgttcttcat cgatgaaatg
       61 gcaagacgtg atattcctct ggaggtattc cacttcgact gcttctggat gaaggagttc
      121 aactggtgcg acttcacatg ggacaagaga atgttccccg atcccaaggc tatgcttaca
      181 cgtcttaagg aaaagaagat cgagatctgc gtatggatca acccctatat cgctcagcag
      241 tcgtcactgt ttgacgaggg cgtaaagaac ggctacttca tcaagaagac agacggcggc
      301 gtgttccagt gcgatatgtg gcagcccggt atggctatcg ttgactttac aaaccctgag
      361 gcttgcaagt ggtacaaggc aaagctgaga gttctgtgcg agatgggcgt tgacgcattc
      421 aagacggact tcggtgagag aatacccaca gccgatgttg tatacagcga cggctccgat
      481 ccttacagaa tgcacaacta ctacacatac ctctacaaca agtgtgtatt tgaggtactt
      541 gaagaatatt acggcaagga taaggcttgt ctgttcgcac gttctgcaac agtcggcggt
      601 cagcagttcc ctgtacactg gggcggcgac tgcttcagcc agtacgaatc aatggctgaa
      661 acgctccgcg gcggtctttc actctgctta tcaggcttcg gctacttcag ccacgatatc
      721 tcgggctttg aagctaccgg ttctcccgac ttatacaaga gatggtctgc attcggcctt
      781 atgtcttcac actcaagact tcacggcaac agctcataca gagtaccgtg gaacttcgat
      841 gaagaggctt gcgatgttct ccgccacttc acaaagctca agggtcgctt aatgccttat
      901 ctgttcgcta acgcagtaaa ggctcacgaa aagggcgttc ccatgatgag agctatggtt
      961 atggaataca gcgacgatcc cgcctgctta cctcttgaca gacagtatat gttcggtgat
     1021 aacctgctta tcgcgcccgt attcaacgag aagggtactg ctcagttcta tctgcctcgc
     1081 ggcaagtgga ccgatatcca gacaggcgag gtactcgagg gcggcaactg gtacgacaag
     1141 aagtacgatt acttctcaat gggtatgtat gctaagccca acagcatcat tgcttacggt
     1201 aactttgagc gtaacttcgc atatgactat gtagagggtg caaagctcgt tatctatcag
     1261 cttgaggacg gcaagaacac tcagtgcaag atctatgata aggacgctaa gcttgagctt
     1321 actgttatgg ctgagagaaa cggcgatacg atcactgtta cacatacagc tacagacaag
     1381 aacttcactg ttgaaagcgc tgaaggctta aaggttgtta taaacaagta aatatataac
     1441 gatgcctccg tgtcaatgag atgcggaggc gtttttttat cgttcacaac tgcggcttga
     1501 cagcatatgc aaagagttct ataatagtta tgtaatgctg acagcggcgg ataatttatc
     1561 tttttcggtt aatactatcc gttaaagctg tttttacgga ggtcagataa catggatata
     1621 aaaatgctcg gcgaatatgc aaagcttatg gtgaaagtcg gagcgaacgt tcagaaaggg
     1681 cagaaggtac gactttacgc cgaggtcgat cagcacgagc tggctgcgct tgtagcaagg
     1741 gagtgctaca atgcgggagc aagctatgtt gagatgttct ggagctgtgg agctatcgac
     1801 agattgcact atgagaatgc aagtgttgaa acgctcggca cggtgctccc atgggaagaa
     1861 gaaaggcaga aacagatggt aaaggatctg ccggcaagga ttttcattga gtcggcagac
     1921 cccgatgaac tcagcggcat acctgccgat gtaatttcaa cagtcggaca gatgcgctca
     1981 aaggttctga aaaagtaccg tgatgagata gacggaaagc accagtggct tatcgttgcc
     2041 gccgcttctc cgaaatgggc caagaaggtg tttcccgatg atgacacaga aactgctgtt
     2101 gaaaagctat ggaaagctat tttctcatgc gtctatcttg acggcgaaaa cgattggcag
     2161 agggtatgga agaagcatac cgacactatg aaagaaaagg cggactggct gaacgctcag
     2221 ggcttcaaga gccttaaata taagagcagt aacggaacgg atttctcggt cgagcttatc
     2281 cccggtgcaa agtggtgcgg cgcggctgat atcaatcgcg taaacggtgc ggcttatgtg
     2341 ccgaatatgc ccacggaaga agtgttcata agtcctatga aaggcaagtg cgagggtaag
     2401 cttgtggcta caaagccgct tagttggtcg ggtaatctga tagacggctt tgaggttgaa
     2461 tttaaaaacg gcagggtatc cggctgtaag gcaaagcagg gcgaggatat ccttaagaaa
     2521 atg
//
LOCUS       DAWAOG010000297         2518 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5375_length_2518_cov_2.253350, whole genome shotgun sequence.
ACCESSION   DAWAOG010000297 DAWAOG010000000
VERSION     DAWAOG010000297.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5375_length_2518_cov_2.253350"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1610..1619
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          643 a          610 c          528 g          727 t
ORIGIN      
        1 cggctcggca ctgaccgaga tatccgagct ttcagctttg aaagaactgt ttacggcaaa
       61 cggctggact atggttacag cgatatgtat ggtcatattc gtactgtttc actttccgtg
      121 ctctacctcc tgtatcacgg tgtacaagga aacgaagagc ataaaatgga ccgccgtaag
      181 ctttttagta cctaccgtaa tcggaactgt actttgcata gccgtgaacg gagcttgtgc
      241 tcttcttggt ataggcggtt gataaaatat ttattcatca cccttaagat accttaccgc
      301 tatctgtgtt ctgtgttcaa gacctgtttt ttcgaggata acacttatat agtttctgac
      361 tgtgccgttt gacaaaaaca gtttttcggc tatttctgcg ttggaacaac cttcggcaat
      421 aagctttact atatcaagct ctctttccgt aagcaggtcg aacacggcac tttttccgta
      481 acactttaca gcggcgtcac ggatatacga cagaatatcc tgctggaata ccgtgccgcc
      541 gttgtaaact gttctcagcg cactgaaaat cccgtctgtc tgtgtgtttt tgagaatata
      601 accgctgacc ccgactttaa gcgcacgctg tataagctcc tgctcgtcaa acgttgtcag
      661 taaaagcggc ttgcacagat tttcttcaag cattatcttt gcggcttcaa tgccgtccat
      721 aacgggcatt cttatatcaa gcaacgctat atcggtcttg tatttcctgc aaagctctac
      781 cgcttctgtg ccgttacaag cgacactgac aatttcaaaa tcaggctgtg acgatattat
      841 cattttaagt ccctcacgga ttatattatc atcatctgca ataattatct tcatatgctt
      901 tcctctttca gactgaatat gtttactacc gaaaatccgt caggctgggc cggaaatatg
      961 cactttccgt ccgtaagcac tgtgcgctcc tcgattgccg taagacccgt acccttcttg
     1021 aactcatcgc taccaccgcc gttatccttg aactcggcac ggatcacctt gttcataacc
     1081 gatattttca gcgaaaattc atctgctccc gaatgcttga gcgtattggt aagcgtttca
     1141 atgagatttt cctttataca gttccatatg cggaatgtga ctctgtcagc gtcgccgttg
     1201 atttcaagcg ttgttcttat gttatatttt gccttatatt caccgagtat ctctttaagc
     1261 tcgcttattc ctattgtttt gctgtcggga cgctcctcac gcagtgcctg ccttatatcg
     1321 tcaacgccgc ttctgaggtt atcggttact gttgtaatac attgctccgc actgtcgggg
     1381 tttgtcctga tgttcagctt tgccgcctca agcaagatta tacttccgga aatactgtgt
     1441 ccgagcttat cgtgtatcct tgctgaaaac cgtctgcgtt cttcgacagc cgcagatttc
     1501 tttgctgttt tagcgtattc ttcaaggctt gctattctgc ggttgaggcg aacagtttcc
     1561 tctctgcttt ctgcaagctg ttcacggcat ttgataaact gtcctgcggn nnnnnnnnnt
     1621 gcccaccgat agcattataa taagtatcag cacatcaata aacggcggtt ttactataaa
     1681 aagaacaagc agtaccgaaa cagcaccaat atgtcggaaa tactttcccg caccgaattt
     1741 atctacagca aagattatgg caaagcacag cacgaatatc agattgtcgc tgtatataag
     1801 aaatgcgata aaagccgaaa cgcacgaaca gaccgccgcc gctttatccg actttttaag
     1861 caccgacagc agatattcag ccagagcaaa gcaggaaaag gcgagtacgg taagcaccgt
     1921 atcgcccgaa tcacagttat tcattatatt catagtgccg aatgcggcag ttgccgtgag
     1981 tttgaataca agatatataa gtgacaattc gtattccttt cgttacgcct ttgaaactac
     2041 cgactttctg ccgaaaagtg cggcgcttgc aagcattata agcaatgtgt agagtataag
     2101 cacgcatata tttatcgcag gctgttcagc ctcacccgac ataaaatcga cataccagta
     2161 ttgcggcgtg atatacgaca gatatttcag catacctgcc gtttcgtcta tcgggaacca
     2221 tgcaccgccg agcagagcgc ctatcgaggt gatacagccc gaaagcgtgg caagagcctg
     2281 ctttgagctt atcagctgtg cgagcattat tgataatgcc gagttgaaca gcgagtagat
     2341 tattacggcg acaaacacaa gcggatagcc ggctgtcatc tgagcgcccg tgataagcag
     2401 gtacagcggt aaaacgataa gtgccgtaaa ggatataagc acgcttgcac caagcgttcc
     2461 gacaatatac tgtacgccag ttacagatga aacagccatt ctgctgtatg ttccgtat
//
LOCUS       DAWAOG010000298         2511 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5396_length_2511_cov_2.065147, whole genome shotgun sequence.
ACCESSION   DAWAOG010000298 DAWAOG010000000
VERSION     DAWAOG010000298.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5396_length_2511_cov_2.065147"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1599..1608
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          628 a          565 c          523 g          785 t
ORIGIN      
        1 atgtgatacc gtccattgcc ttgttagtag agcttaccac aaagtcatag agtgttctgc
       61 cgacgagaag ataaacaggg aaattgggta tattcctgtc aaacagttta gagaatacaa
      121 catacataac tatcattatg agaagcggat taagaacgct ccatacataa ccgagtacac
      181 tgcgtctgta tttcagctta agatctcgtg taacgagctg tataagcaga tccttgtagt
      241 gccttacttc aagaaatccg ccggttttat ccttgcgaac aagtgtcatt tttacctccg
      301 tcagtcaaag ctcttattgt gtcttacttt gttataaagc tttgaattaa gaattttatt
      361 gtatccgtta agtcctatgt gcagtctgtt gagcgtcttt aatatcttac agtgctttct
      421 gctcatttca tataccgtat gatattcctt gtccataaag ctcatcattt catccgctct
      481 ttttctgcct gcctttctgt ccttatcgca aagcagggaa gtaacaagaa agctcagcag
      541 gagcagatgt gttttctttg cacaataaag cgaagccgca ccggtaaagt ctttgccctc
      601 tgcggtcata cgtttaagca ctgtttcggt gtgccctatg cgcttgagtt gattttcagc
      661 agacacgctc tgcgtaacat cgcctatgcg gtattcgtaa atgaacagat caagcggcaa
      721 aatgcttttt gctttactgc acgggaatgt agcatactcg tgatcctcat agaacacctt
      781 ttcgctcatc tttattccct cgcttctgta gaagtcggtg cggtaggtta taccgtggaa
      841 tgtcagactg cggtcaaagt ttttccactg agtcattatc tcgtccatag tgtattcttt
      901 accgaactgc ggcgggaagc aacgccagct tttaacttcg ccgtttgaaa tatttattgt
      961 atggtgcggt gtcagaacga catctgccgc agtgttttca agcgccttta caaatgcggg
     1021 tatgttttcg gtgagaaacc ggtcgtctgc gtcaagcacc ttaagatact tgccccgtgc
     1081 cttttcgctt ccgacgttta tacctgagcc gtgaccgccg ttttccttgt ttataaggct
     1141 tatcgagccg gggtattttg caacatattc ttcagcgatc ttttcacacc cgtccgttga
     1201 accgtcattg acgactatta tatcaagctt gtcgatacat tcgcataaaa gcgaatcaag
     1261 acattttgcc aaaaacggtt tgctgttgta ggacgggatt ataatactga gtatcttatc
     1321 cattattttt tacctttttt aagtttagtc agtctgtttt ccttgatacc ttttaccgta
     1381 tatgaaaaag cccttaacat actcatgcct gagagctgtt tcctttttgc aaaagcatag
     1441 tacgcggcgt aaagtaccga cagcggcaga cccattatat agcgcgttgt atttccgcgg
     1501 ttgacaaaaa actcctcatc aaagcctttg aaccatgttg actgcgtctg tgccaccgat
     1561 gccagcctga acgggtagtg atatatcctg agctttgcnn nnnnnnnngc gtcagaaagc
     1621 ggaactcctc ctctgcaccg ttgcctgagc ctgcacccat attttcgtcg aacagtacgc
     1681 ccgaggcaag taacttttcg cgcctgaatg ttatctgcca cgagcttaca ctcataaggt
     1741 cgatataacc gagcctcttt atgctgttgc cccatttgag cggtcggtcg accatatcga
     1801 atattatgat atccgcatcc gggagcgcgt cataagcgct cgatacggct ttttcgtatc
     1861 cttcattgaa tatctcatcg tcatcgcaga ttatacatat atccgcctgc gatttcgata
     1921 ttgcgaggtt gcggcttttg gtaagtcctc tgtcagtcac cgaaaacgtg cgtagaagcg
     1981 cgttgcagat attctcttct ttatagtttt cctcatcgca ctgatttatt ataacagtgt
     2041 ccgaggtgat atgagaacgc tttatcattt ctttgctgtc ggaaaggaac atacacgaca
     2101 gaagtatctc agtctttgcc aatttttcag ctccttgaat tacggcaata tacggttttg
     2161 ccgtaagcaa catttattct attatacaac ggtcgctttt aaaagtcaat cgacattatt
     2221 ctatagtatt ttgccgcata gacgacagaa tatatgcgtt ttacataaaa aaggggttgc
     2281 aacgcaacct gcaaaaagaa aaagtgagcc gaagcgcaaa agatagtgcg ctcggctcac
     2341 ttttttgcta tgtggagaaa ggttgccttt agtacgtttc agttataccg tattagtgat
     2401 taacattaga tagcagtcta ccgatagtgc ttttcgtggg atagcacttc tcgggttcgc
     2461 tattccattt gataacgttg ctgctatgat tgagggagac aacccaaggg g
//
LOCUS       DAWAOG010000299         2511 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5394_length_2511_cov_2.141694, whole genome shotgun sequence.
ACCESSION   DAWAOG010000299 DAWAOG010000000
VERSION     DAWAOG010000299.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5394_length_2511_cov_2.141694"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    471..480
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          759 a          474 c          596 g          672 t
ORIGIN      
        1 cgatagtgct tgacatccct tttaacaccc ccactacagt aaatttcagc gaatttcagt
       61 aaaattcatg ttttgataat gattactgaa aatgcaagaa aaccgcttaa gcaatacgct
      121 taagcggttg tttctatgga gcttgtgacg ggactcgaac ctgcgacctg ctcattacga
      181 atgagctgca ctaccaactg tgctacacaa gcaaaatcat tttatttctg tattacttac
      241 agcaaaatgc acttgaaata agtaacggaa tatgataaaa taactatagt cgtatttgta
      301 agaatgctta aaatcgacta tataattata tcgtattttt aatttgttgt caagagcttt
      361 tttgacggaa atgaggcaaa tttgataaaa aggtcatcgg ttgcgagagt gttcggtatt
      421 gtgatcggaa tagtgcttat tgttttcgcc gtattcctgg cagttacatc nnnnnnnnnn
      481 gatgaggtaa acaacggaga actgataata tataatcgct atgagaacgg cggggcgcaa
      541 ctcgcaaagg ttaattcttc aacgctcaaa gagggcgtta tgagctttga tatagaaaca
      601 caaggcggcg agaacatcac gatttcacaa agccggcttg ttgcaagggt caactattgc
      661 agtgattttc tgggtgctgt gataggcttt gctatgtcac cgtttggcgt aatggcaata
      721 gcaatacttc cttgccttgc gatagttgct tttgagatag tcaaattcat attctcaaag
      781 cgtcctgtac caaaggtaga acccgtaaag ctccaggaag aaacccctgt atatacacct
      841 gctgcgccca aagaacggga gcaggcaaaa atcaatctta acaaggctaa gatacccgct
      901 atatcggata aaccgggctt gacccgtaag ccgctgacgg acggcgctca cagcaagacc
      961 gatgaattta ccgatcggtt aagggtaaag cggaatgcta cggtcaatgc cgatgagata
     1021 agggacagag cggatttttc aacagctgca aagcgcagaa tgtctgagga caggagcttg
     1081 acggaaaaat cggcgaaacc tgcaggaact gtttccgtga acgccgcacc cgacagcaga
     1141 cccgctcata agccgacttt aattacagaa aagaaagaag tcgctgaaag atttgttccg
     1201 gacaatacga aaaaaacggc ggcaggctca gaacctgata tatcgcttgt ttttcaagat
     1261 gaagacgata agagatatga tatagatgat atccttgccg atatagagaa acgtcatcat
     1321 tgacggacag aaaagtgaga acgaaagagg catcctttaa atggataatt tgcttgattt
     1381 taaaaattcc gatctgcgga ctaaattact tgcagttctt gcgatcacag ggtgtatatc
     1441 caatatagcc ggttttacgg caaatgccat tctgtttggg tttacaacgc aaacgcttat
     1501 gtgtgcatta tgcgcagtgg tcataataag tgcatctatg tatggttttt tcagcggaag
     1561 aacgtatgtt gcaacgttca taatactgct tatactggga tttatcgaat ttccgttgct
     1621 gtatatttcc tacggcgaaa gcacaatggt gtatatgttg gttgcggaaa taggaatagt
     1681 aacctttatg aaatcaggtc atagggtcat aatgggaaca gcggcattca ttgttgatat
     1741 cgcggctttc atagtacgcc atacttgtcc gcagttcaat gttgcttcgg acagcttggg
     1801 tattgacaca ttgatatcgt ttataatagc tttttttgca atacttgttg cagtggcaat
     1861 ggtaataatt cagtatgagc aacaggcaag ggaactgagt cgcatgggtg aagagcttac
     1921 gcaggcagtg aacacagatc cgctcacaga gatcagaaac agacgttatc tcattcgtta
     1981 tctggaaaat cagacggcag agatggcgga cgaaaagcac tatgccgtac ttatagatct
     2041 tgattatttc aagtctgtca atgacacata cggtcatgtt ttcggcgaca gggtcttaaa
     2101 aaagtttgcc gatacggtca aggaaaatct tggagaaaaa gatctgttag cacgtttcgg
     2161 cggcgaggaa tttatgatac tgtttgcaac ggataacgcg gaagcggttt tgacggtaat
     2221 gaaccgaata agcaaggagt atgctcaatt ttcggagaga gagaaaggca aggagttcac
     2281 attcagcgcc ggagcggcac tgtacggcag gaacagctca ataacggata tattcactgc
     2341 cgccgacaaa aggctgtata aagccaaggc agcaggcagg aatatgattg tgacggattg
     2401 aggaatatta tacgccccgc ttttttaagc ggggcaatgc tttttaacgt cggcgttttt
     2461 cggaaatgcc gacgttttat ttaactgtaa ttttaagtac ggctcttgaa g
//
LOCUS       DAWAOG010000300         2511 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5398_length_2511_cov_1.611564, whole genome shotgun sequence.
ACCESSION   DAWAOG010000300 DAWAOG010000000
VERSION     DAWAOG010000300.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5398_length_2511_cov_1.611564"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    220..229
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2225..2286
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          572 a          663 c          458 g          746 t
ORIGIN      
        1 gtctggcgga aatagatata ttaaatctgc ttggcggaaa tcccgctatg caataaaatg
       61 aataatgcaa agaccacact cgttgtaagg gtgcggtctt tttgtattat cggttataaa
      121 atcagacata taagtccgtt gcagccgtca ttgataattc tctgtattgc ctcctggagc
      181 tttgccctcg catctgcggg catacgactg agcttattgn nnnnnnnnnc tcgttcacca
      241 gctcgtgcag cgactttcca aaaatattgc tctcccatat cttggtcgga ttttcttcaa
      301 actctctcag gagatattta acaagatcct ctgactgctt ttcgcttccg acaataggat
      361 ttacctcagt gttgatgttg gcaagcgtca tatggataga gggcgcagac gctttaagcc
      421 gtacaccgaa cttacccccc tgctttacta tctcgggttc ttcaagagta agctcctccg
      481 cgcccggcat tactattccg taaccggtag ctctcacttc ttcaagagcg gcacgtacct
      541 tctcgtactc atttttgatc tttgcaagct cgatcataca cggcataaga tcgccctcat
      601 cggcaagctc aataccggtg gtttcgccga gcaccttata aaacagctcc tgatcaagat
      661 ttaccgtgat aaatgccgct cccgtgccta gctgtgcgtt atccttttta caacagctga
      721 tgctttcaca ctgctttact ctgtcgataa acccgtctat atcgcccata cagttaatat
      781 cttcagctgc ctcacgtata gaaccgaata cctgcttttt gagataatga tcattgtcaa
      841 gcgatgcaag ccatttaggc agtcttatcc cgatctccct tacaggaaag cccataagta
      901 tatccgacaa caatgccttt ttatcactct ctgtgatatc cgggcagctg agcggtgtta
      961 ccgcacagtc atatttttct gacagcttac gcgcaagttc tgccgattgt tcggatttcg
     1021 gctcagtaca gttaagcacc actgcaaacg gcttttttat gcttttcagc tcatttatca
     1081 cgcgctcttc cgccgcctcg tattcctccc tcggaatatc ggttatactt ccgtctgtag
     1141 ttaccacaag tcctatcgta ctgtgttcgg ttattacctt gtgtgttcct acctccgccg
     1201 ccatattgaa tggtacttct tcatcaaacc acggagtttt caccattctc ggcgcttcat
     1261 tctcaatata gccctttgcg ccgggcacga tatatctacc cctatataga tatttccgcc
     1321 tgttcgctcg gaaatgtcct tataaattga ctttggcata atttctcctt atctgctttc
     1381 acaatgcctt atctccgtgc ggaaaacaag actttcaaac tatcggtatc agcagaagcc
     1441 ccgacgcctt tccgtcggtg acttcgttct cggcttctat tgccgcggca gaggtattat
     1501 agcgtttcgc tatattccat atatcttcat tcgagtcggc aaaataaagc ttaagcgtat
     1561 actcatcatc ttttttctta cattcgttct catttatttc cgcctgcttt attacctgtg
     1621 aagaagagca aatatacata cttccctgca taagcatatt gcatctgagc tctatcccgt
     1681 tatcacctat actgtaactt acagctgtta tactgattgc cgactgaatt acgctgtctg
     1741 ccgtaactcc gccgaccgat accaccgcct caaacggcag gctcgtctcc gagaatacag
     1801 gctcgttttc tcttacagcg gcgatacagc atatcaattt accgttcacg gtaatatctc
     1861 cgttctcgcc agccttgcac agtacctcag ccacatcaca gctgacatct ataacactgt
     1921 ccggaatacc gttctcacat tcgataatcc gctttgtgtt gcaggtctga cttatgtaac
     1981 ggtgatccga ctctgctttg agtgtcgccg ttgtaaaatt acactcgtac cctgtagaat
     2041 acatatctgt taccggatat acattttctt caagagtagc ggttatctgt gagcctaccg
     2101 tgagttcgca ggcaaaaaca cccgatgtat tgtcgtctgc ggattttaca caaagactgc
     2161 atgacaggac cttgaactgc gcatagcagg tatgcttttc atcaactccg ggcatatcca
     2221 ctatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnncata gtctgtagca tgctgccgcc gtcattttgc acaagataca ttgctttgag
     2341 ctgagccacg cctttgagca caactttatc ggcaattatc ttgcagtcgt ttaccgttgc
     2401 aaccgcatca cagcttacta ctgccttgat attgccgtca acgccggata cccctatatc
     2461 ctcacgtatc acaaactgtt ttcccccggt gagctttctc gtacagcaga c
//
LOCUS       DAWAOG010000301         2505 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5415_length_2505_cov_2.257959, whole genome shotgun sequence.
ACCESSION   DAWAOG010000301 DAWAOG010000000
VERSION     DAWAOG010000301.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5415_length_2505_cov_2.257959"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    549..586
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2094..2103
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          644 a          548 c          531 g          734 t
ORIGIN      
        1 ctaatattgc tttgtatttt cgctatcaga aaaagaaaat cacggcgata gccaaattgc
       61 tatcgctgtg aaaatcagaa aatttattgt ttatctttgc ttgctgtttg tatttttatc
      121 tgttacgcat ttttctcttg ctaagcggga acacaagcac aacagccgca ctcatcatta
      181 ccattgatat gataataacc gctgtctgcg taaagctgtc ctcgcttgca cctgtcgggg
      241 gattgtccgg tagcttgctg ttgtgcatat tggcaacaag cattgcaccg tcacggtcaa
      301 tcgttaactg cttatcttcg ggtaaaagat agcctgccga agcagaatcc ttaacttcgt
      361 gtatggtata ttcaccggct cttaaatcgt taatgctgat tataccgttc ttgtcggttg
      421 tgtatgtttt cttgaaagtc tgacctgtgt atgtctttcc ggttacctca aaatgtattc
      481 cttcgacctt gttgtcgtat gaagtcttta caatcttgat ttcgcccttt tgaggtgagt
      541 tgataaacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatag gcgatgtata
      601 gtcgaaagga gcgctcttat ggcgaaagca agtaatggca aaaacttttt taacgagatg
      661 actcgtgtcc agatgcctgc acttgttcat ctgacaaggc tcggatatac atactatggt
      721 cgaatattcg aggatatggc tgatgaggtc tatgatcagg atacaaatat attgaaagat
      781 atttttatat ctcagttcaa gaagctcact tctatgtgtt cccctgagcg tctgcttttc
      841 attctcaaat atggaacgtg aagttgatgg caagattgag actatcaatc agaagcatat
      901 tatgcggtat cagcagatgt tcgcagcctt tgctatccgt gaaaagctct ccgagggtgt
      961 caaatcgggt gtcgtatggc atacccaggg cagcggtaaa acagcctcct aaaaatgaaa
     1021 tccgcccatt ttgaacaagt caaaacagac tgattttgat gtaagaatat gcttttaaga
     1081 gcattagggc gcacttatcc cactgaataa aataattatc cggcggatgt gccatcgtcc
     1141 aaagcatatc ctcttttaca atatcattat atgttttttg agggtgaatg tggtcaccgc
     1201 aacggcgaca aaaagcgccg gtcaaaatga ccgacgctga aaaatgatta ttgattcagt
     1261 cgaacataac accgggatta atctgtgccg gagaacgcca aataataccg tgttcaaaca
     1321 gatattccgc ttttaagttc caatattcca tacagaaatc cattgttttt tctttgtcta
     1381 ttcgttcctc tacctcgtca atcacagcaa gatattcctc cgttttctca acaggatcgt
     1441 ttttgatgaa tcccagtcgg ttttcctgcg ctgagagaag caattcacga attctttcat
     1501 ccggcagact gccggacacg attgcgacaa ggtcggagca atcgtcaggt tcataataat
     1561 ttcttgcttt gaccgccaaa taaaaacagt tttcaaaccc ttccatgtcc tcaatgtcat
     1621 attctctgag tttcataagc tctgtatggg cttgaagctg cagtgtgtaa aatttcacgg
     1681 acattcccgc acggcacatt ttgtcataac attcggtaag ataccaaagc gtcggaatga
     1741 tctcttccag cgtttctata tcgcgatcac ggggagcaag cgcaatttgc gccatttcgg
     1801 caagataagg gctgtcttta taggtgggcg ctgcgtggct gtgctcctta tggccttcaa
     1861 taatgccgtg aagctttacg attctgttta attcctgcat ggaaatgggc atagtatacc
     1921 tcccgtttat aggtctattc tatcatagcc ttgataaatc cgatgatatt ctgggcggcg
     1981 ttatagcgaa gcatacctat accaaaggaa aattcgtcac ggcgctcttt tgaaaacgga
     2041 aacagtagct tgaccgagta gtaaggctcg tctgctttgt ccgctataat tttnnnnnnn
     2101 nnngttcgtc ctcatcccgc gcaatcagtc cgtttttata gaaatagttg attttgttcg
     2161 gctcgcactt aggatacagt tccgtatcaa agcggtgatc ggcggcaatg tcggcatcgc
     2221 acagattaaa ataatcataa gccgctccgc cgaaacggga atcccaggct gcatcggtat
     2281 gggcggttac accttttata cgggtgatgt tccaggaatg aggctcccgc cgcccgtttt
     2341 cgtgtaaagc ctctcctaca accacaattg aggctattcc aagtctgtcg cacaacagtt
     2401 tatatgcctt ggcgaatcct tcgcagacgc accgatcgcg gagcaatgca ccgaccaccg
     2461 aaaagcagtc gctgtcaaat ggctcctcgg ccgtgtgaat ggtgt
//
LOCUS       DAWAOG010000302         2493 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5438_length_2493_cov_1.737490, whole genome shotgun sequence.
ACCESSION   DAWAOG010000302 DAWAOG010000000
VERSION     DAWAOG010000302.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5438_length_2493_cov_1.737490"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    608..619
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          411 a          713 c          535 g          822 t
ORIGIN      
        1 gtctttgcta tttcctcggt ctgagctatc ttctgagata taacgctgtt cttctgttct
       61 atgccgttct tcagctcttc tatttttttc agctgttcgc ttccgccgtc gctgagtgca
      121 agaattgatg cgtctatgcc ctctatcctt gtgcggatat tcgatatatc gccgttcacc
      181 gacagaatat ccatattgtt cctttgtatc ctgtcggcaa gcgtttttct cttgtcctca
      241 gccgcatcaa gtccgcttct ctgctcggat aacgccgctt cattcttttc tatctcggca
      301 agtacgcctt caagctgtga ctttgtatcg gaaagcagct tcctgccgtc attctcggct
      361 ctttcctgtg cgtcaagctg ttcctcggcg ctgtcgcgct gtgcggtgag ctgtgacagg
      421 agctgtttta taccgtcacg ctgtgcctca agtcgtgcta tcttaggctc gcactgcgat
      481 acagtttcgg caaagccctc catctcagcc gccatctttg cgacttccgc ctgcaagggc
      541 tttagctgct cactttcgtc ctttatcttt gcattcagct tttcgatctg ctcggataac
      601 agcgcaannn nnnnnnnnnt tcttacgctg ccgcccgtga acgaacctcc cgcgttgatg
      661 acctgaccgt caagcgtcac tatcctgaaa cggtatccgt acttcttggc aatgaacgat
      721 gccgtgtcga tatcctcaac taccgccgtc tttccgagta tggattttac tatgccgtca
      781 taaagtctgt cgtattcgac aaggtcgcaa gccataccct caaagccgtt ctcactgctc
      841 agtccgccga cctcgagcat ttttcccttt acgcttgtca gcgggaggaa tgtagcgcgt
      901 ccgaggttgt tttctttaag ataacgcata ctgcgctttg ctgactcttc gttatcggta
      961 acgatgttct gcataatgcc gccgagggct atttctatag ccacggtgta gcgcttgtcc
     1021 acgctgatta tatcggctac cgtacctctt atgccgctga gccgtccttg cctgtcagcc
     1081 attattaccg actttacgct tgactggaag cctgccatat tcttgtctat ctcgtcaagc
     1141 acctgatgac gtgaggcttt cgtttcgtgc tccttgcgga gcgtttcgag cgtctgaccc
     1201 gctgttttca gcttgtcatt cttattggca aacagttttt tgtagcctgt cagcttattc
     1261 tcggcttcgg ctttttcttc cttcgcctgc tcaagctctt catctgtatc ggtaagcttt
     1321 attctctgcg cctttgcttc tgccgcggca ttttctgcgg ttcttgtaag ctcctcctta
     1381 cgctttgaaa gctgttcgga ggtcgactct atctgctgtg ccgaaagctg gagccgggtt
     1441 cttttaagat acagctgtcc ctgcttttcg tcaagctgtc tgtactcgcc cgaaagcgtt
     1501 tcaccctcgg catcgatgcc ggtaagctct tctgtcaggt tttcactgtc ggctttcagc
     1561 tgtgcgatga acgcctgctt ttcttctatc tcattttcga ggtttttctt atcctcttca
     1621 agccttgcct tgccgctctt gccgtcctcg atctgctttt cggtttcagc aatagcttct
     1681 tctgcgtggg aaatgtcgtt acgcagtacg gctatctccg actttatatc gcttatgcgg
     1741 tcttttgccg cgtcactgcc ggctttcagc ttttcaagct cggcacggag cgtcattctg
     1801 cggttgttgt tgtcctcttc ctcggcttca agctttgcta tatccctgtc gaggttttcg
     1861 cactgtgcct tgctgagaag aagcttatcc tctgcttccg aaattgtctt ttcgagtgcg
     1921 gacagctcat acatacttac cgttatatca aggtcttctt ctcttgcaag cagttcctta
     1981 gccttttttg cctttgccgc ctgcttttca agcacgggga gtcttgtttc aagctccgac
     2041 gctatatcac ggatacggag cagattatcc tcggtgcgtt tgagcttgtt ctctgcgtcc
     2101 tttttctgcg ctaagaattt tgatacgcct gccgcttctt caaacacctc gcggcgctta
     2161 cttccgcccg attctactat ctcagatacc ttgccctgac cgataacgct gtagccgtca
     2221 cgaccaagac ccgtacccat aaaaagctcg tttatgtttt tgagaagcgt tttggctccg
     2281 tttatcttgt actcggactc gccgcttctg tacagcttac gggatattac aacctcgtcg
     2341 ctgtctactc tgagcgctct gtcgtgatta tctatagtca atgttacgct tgcaaagccc
     2401 ataggctttc tgcgcacagt tccgtggaag ataacgtcct ccattttatc tccgcgcaga
     2461 cttttcgcgc cctgctctcc cattacccag cgc
//
LOCUS       DAWAOG010000303         2486 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5462_length_2486_cov_2.099136, whole genome shotgun sequence.
ACCESSION   DAWAOG010000303 DAWAOG010000000
VERSION     DAWAOG010000303.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5462_length_2486_cov_2.099136"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          801 a          537 c          538 g          610 t
ORIGIN      
        1 tgagatgggc gttgacgatt atgtggtaaa gcctttttct cccaaagaag tacttgcgcg
       61 cataaacgct ataatcaagc gtaataagtc aggaaacgaa cctgtcggcg aaacagtcaa
      121 gtttgaaggt cttgagataa acttcactgc aagagatgta tttatcgacg gagaaaaggc
      181 aaaccttact cccaaggaat acgacttgct tttctatctt gtgaaaaaca agaacatagc
      241 gcttacaagg aacaagcttc tggaggaggt atggggatat gatttcttcg gcgatgacag
      301 aacgattgac acacacatca aaatgctgag aaacaacctc ggtccgtaca gaaagttcat
      361 tgttacttta agaggtatgg gatataagtt ccaggtttaa aaataactac agccgtcagt
      421 gttgacggct gtaattgata ataaggaagg cacttttgat gaaagacaga ttaaacagta
      481 tacgttttcg ctcgtggttt atttttgtgc tgttctcact gcttgttata gtgttcctgt
      541 atttttttca ggtcgtactc ttgtcaagct attatgaata tatgaagata caagaaaccg
      601 ccgccgccgc aaaacagata aaggacgcat ggtttcagca gcccgacaat ctgaacaaca
      661 tcgtatcgaa tattgccgac acacaaaaga tatatattga gatatccgac agcaaccgac
      721 cgtatttcag ggcaagctct gtggataaaa gcaataagga ggtctactcc ctcatcacaa
      781 gcctagaccc ggaggatatg aaagaggtga tttcaggaga aaacacattc ttcaccgcca
      841 ctatcccgag aacaaaaaac gacagcaagg ctatagttct gataagccag cttgatgacg
      901 gaacagaaag cacatttttc aatacaaacg gcgtagtaag actgtatata ttcaactatc
      961 tcgaaccgct cggaacaacg accgatattc ttcacagcca gcttaacctt acggcaggta
     1021 ttatcataat tgccgcagtt ataatgtctg tgattttttc taacagcata tccaagccta
     1081 tcatcaatat atcaaaagcg gctctgaagc ttccgcaggg acagtttgat atgccgaagc
     1141 aaagacggca gtttaccgaa ataaacgagc ttaccgaaac actcagctcc gcatcggtag
     1201 aaatagcaaa agcggatacc ttgcgtaagg atcttatggc taacatctca cacgatctgc
     1261 gaacaccgct tacgatgata aaagcctacg ccgagatgat aagagatctg tcaggtgaca
     1321 atcctgaaaa acgtgaaaag catttgcagg ttataattga cgaaaccgac aggctgactt
     1381 cactggtaac agatattctc gacctttcaa agcttcagag cggtgttgcc gagctgaaat
     1441 acgaaaagat aaacttctca gagcatctgg gtgatatagt cccacgcttt tctctgctca
     1501 acgaaatcaa ggactacaac gtagttctca atgcagaacc ggatattttc ataaatgccg
     1561 atatcacaaa gatcgatcag gtagtataca acttcataaa caacgcactg acttatacag
     1621 gcgacgataa gacggtaaaa gtcaatctct accataagtc gcctaaaacc gtgcgtcttg
     1681 aagtctgtga cagcggtata ggcatcgacc ctgaaaacct taaatacgtc tgggacagat
     1741 attaccgcgt caaaaaagac ggagaaacac accaacgtgc aaagaaagga agcggtcttg
     1801 gactgtccat cgtaaaaggt gtgcttgaaa tgcacaagtt caatttcggc gtggacagta
     1861 cggtcggagt aggaagcacg ttctggtttg aatttgagga ctgcaatcct gatatagtac
     1921 ctgctgacga aaaaaagcac aaaaagcaaa aaaagcaaga ataacaccga acaacagtaa
     1981 tacagcaagc ccccgcttga taaaaagcgg gggcaagtta tattcaaaaa acaattgtca
     2041 gtcgcctgtt gactctctga gcacaagact atgcggcaac gtgtaaagat tatggtcagg
     2101 tgcagttgct ttcatgttct caataagctt ttcgataacg agcttgccct gcgcataaca
     2161 cggctgctct actgtcgaca agtgcggcac gaacatatgt gagtactgga tattgtcaaa
     2221 gcccataaat ataagatcct tgccgatcct gtagcccttt gaaagcgcat agttcatagc
     2281 accgatagca ataatatccg atatggagaa tatagctgtc ggagggctgg gtaaattcat
     2341 aagatactca cagcctctca taccctgctc tgtttcatag ccgccgaaat aaacgtaatc
     2401 ttcgttatag ggaatgccgt tgtccttgag cgcaagcttg tagcctatct cacggtcgat
     2461 gcttgactgg cttctctgca atgtcg
//
LOCUS       DAWAOG010000304         2480 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5479_length_2480_cov_2.818557, whole genome shotgun sequence.
ACCESSION   DAWAOG010000304 DAWAOG010000000
VERSION     DAWAOG010000304.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5479_length_2480_cov_2.818557"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          567 a          680 c          520 g          713 t
ORIGIN      
        1 ccttatttca aggtccttca ttgctatcct gaagcccgag ccgaagcgtg taaactccct
       61 tatagcatcg agtcgcttag ttgcgacctc actcagcacc ttgccgcgtt tgaacgtgaa
      121 atatgcatat gcacgacggt ttgtacgtcc gacacgacct ctcagctgat ggagctgggc
      181 aagtcccata cagtctgcat tctcaatgat aagcgtgttg cagttaggaa catcaacgcc
      241 ggtttcgata agcgttgtgc agacgagcac atctatctca ccctcgataa gccttctcca
      301 tacatcgagc agctcttcct cgctcattct gccgtgcgct atgccgatat tggcatcagg
      361 caccatagaa tgaatatcgg cggcgcagga atatatactt tctatgcggt tatgaacata
      421 atatacctga ccgtttctgc gcagctcctt gctaatcgcc tgtgctatta cacccctgtc
      481 gtgctctacc acataggttg ttatcgggtg gcggtcgccg ggcggagttt caatgacact
      541 catatcgcgt attccggaca ttgccatatt gagcgttctc ggaatagggg ttgcggataa
      601 ggtcagcaca tctacatttg tgaatgcctc tttcagcttt tccttatcat ttacgccgaa
      661 acgctgttcc tcgtctataa taacaagtcc gagatctttg aactttacat catcctgcac
      721 aagcctgtga gttccgacaa caaggtcaag ctcaccctct tcaagctttt taaggatagc
      781 tttctgctcc tttgcggtgc ggaaacggga taacagctca actcttatgt tgaagccctc
      841 catacgcttt aaaacagtct gatagtgctg ccatgccagt acggtggtcg gcactagaat
      901 agcacactgc ttaccgtcac ctatacactt gaatgcggca cggagcgcaa cctcagtctt
      961 gccaaatccc acatctccgc acaacagtcg ctccataggg gcagtacgca tcatatcatg
     1021 ctttatctct tctatgcatc tgagctgatc gtccgtttcc acatagctga aacggtcctc
     1081 aaatatatgc tgttgctcgg tgtcgggcga gaacgcaaag ccctttgctt tcatacggcg
     1141 cgaatacagc tcgataagct ccttcgccat ctcctgcgtt gccgctttcg cctttatctt
     1201 tgcctttttc catgtgtccg taccgagctt attgagtctt accgtgtccg cgtcaccgct
     1261 tccgatatag cgtgaaataa gatcaagctg ggttacggga acgtaaagca cgtccgcacc
     1321 tgcgtatttt atcttgatat agtcctttga tatcttgtcc gctgtgagct tctgtacacc
     1381 ctcaaatacg cctataccgt aagagccgtg tacaactaaa tcgccctgtg agatatcact
     1441 aagcgaacgt atctgcgcac ccttcttgaa cttcttttta ggcgttgccg tctttgaaga
     1501 agatgcactc gttcttgtga taacggcgat cttctcctcg ggatattcaa agcccgccga
     1561 tattatgccc tctactacgg ttattcttcc cagatagaat ttcttggggc tttttgcgta
     1621 gtctgcctga tatccgctgt cacgcaggtc gtccgcaagt atttttgccg ccttgtccgt
     1681 accgccgaat ataacgcagg tatagccgtt cttcttgtag ttgtcaagct cctcacacaa
     1741 cagcttgtat tcaccgctcc acggagaaag ctgtattgaa tcgactttca ggatatcacc
     1801 gaggtcaata ccgccgcctc ttatgaacgt atccatatag atacgcacgt tattcttgac
     1861 agcgtcctga tattcagcct tgctcataag gtatctgtcg agtcccttac atagaactcc
     1921 gtcgtcggtg agcagtttga gatcctcaag atgagctgat atcgcaccct ttgcagactc
     1981 tgcggcggtg aatgattcac agacaataag cgtatttgca taatcgaata tacttcccgc
     2041 cgtcttgtag cacaacggga aatatcttgc aagacagcat accgataatc cgtcacgcag
     2101 tcttgctatg tcacgcctta tacgctttgc cgcttcatcg tttttctttt tgttcttctc
     2161 ggtcttttca agaagtgtct gcaacacctc cggcagaacg ccctcttcaa atatagtttc
     2221 tccgcaaggc gttatactga cgctctttac agtgtcaatt cttctttgcg tatctatatc
     2281 aaacgatgcc accgtgtcta tttcatcgcc ccacagctca attctgacgg gaaaatcact
     2341 gctgacagga tagatatccg caagcgagcc gcggaacgag aactgtccct tgccctctat
     2401 catatcgcaa cgtgaatagc ctgcggcaag aagtgttttt gcaagctcat caagctttat
     2461 ctcatcgccc gctttcagcg
//
LOCUS       DAWAOG010000305         2475 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5498_length_2475_cov_1.787603, whole genome shotgun sequence.
ACCESSION   DAWAOG010000305 DAWAOG010000000
VERSION     DAWAOG010000305.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5498_length_2475_cov_1.787603"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1285..1294
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          604 a          631 c          499 g          731 t
ORIGIN      
        1 ttgtgcagtg gctgatgccg tcaataagat catcgtctgc ggcgaaaacc agtacctctg
       61 cgtgacgtgc ctttgtgctt ctgttgcctc tgagattagc cgccgcaata gcatttacgt
      121 ttaccgcaaa gcctacagcc ggcatatcgt ccgctccgaa actgccgata agcgtatcat
      181 atctgccgca cgaaagcacc gcctgaccgt attcctctat atatcctctg aatacgatac
      241 cagtataata ctcagccttg tttacaagac ctaagtcgac tgttatcctg tcctcggctt
      301 caagctttct cagtgcgtca aacgtttcct taagctctgt aagtctgtcg gaaaggtcag
      361 tgcccgcaaa cagcttttca gccttgtcaa acacctccgc gccgccgaac agctcaggga
      421 gctgtaaaag tatctcggaa tatctgcgct tttcgggagc gttatccaca ttgtccttta
      481 taaactgtgt aagatcggga gtgttcttag actcaataag acttcttacc gtttcctcgt
      541 cgtcaatatc aagcttttca agaagcagtt tatagaacgt gttatctcct atttcaagac
      601 gtacatcatc actcgagcag gaattaagca cctgagccgc aagactgagt gcctcaaggt
      661 cggaacgctt ctcgtcaccg ccgattatct caacaccgct ctgcataaac tcatcgtccc
      721 tgcctgccat cttaggattg caacggtaga tattctgtga gcagaacagc tttaacggaa
      781 acacgccgtc acgcagtctt gttgcgacta ttcgagcgat aggcattgta ctgtccggac
      841 gcattaccat aagtctgccc ttgccgtctg tcagcttata catatcctcc tggcggaagc
      901 ttcttgtctt gttgttgaat acatcgtaaa attcaagcgc aggagtaatt acctcgctgt
      961 atccgtgggc ggtgaacacc gctcttaatt tatcctctgc ttccttacgg gctacacact
     1021 cgtcaaacag cagatctctt gtaccctcag gtgtaataag atcgtagctt ttcatctatg
     1081 gttatttcct ttcatgcctg tcggctttgt ttacttttta agtcgttcac gttgcgtcag
     1141 ccaagccgac gctttagtgt gctaacgtgc tatcacgata atatgataac acataaaacc
     1201 gattttgtca atccccacag ttatatcagg gaaatttcgg cgtttgttac aaaaagtttt
     1261 tgggctagta tcaattattt gatannnnnn nnnncccctg tccattccgc cgattatcag
     1321 cgtatcggta tcaggatacg ctttaagtgc ggctatcgcc gtctgcggcg ctgtgcttat
     1381 actgtcgttt atatactccg caccgttcag tgtgcagaca tattcaagcc tgtgctcaag
     1441 accgttaaac tgagggagcg catcaaggca ctgctttacc gtacagcctg cctttgttgc
     1501 ggcacagata gctattgcaa tattatacag gttatgctca cctttaagct ttgtatctat
     1561 catatcagca ggataaaact catcgccaat gaatataccg ccgtttcttg ttcctatatc
     1621 accgcaggaa aagcccgccg ttataatttt tgcctttgta tcggcatact gctttacctc
     1681 ttcaccgatt atcagcgtat cgttttcgct ttgatagcgg aaaatgttca gctttgcgtt
     1741 tctgtaagcc gcaaaatcgg tatagtggtc aagatgttcc gggtaaacat tcagcagtac
     1801 cgccacttca ggcgatgcct tgacatattc gagctgatgg caggacattt cacatacgat
     1861 aatgcagtcc tttgtgaact cctcacgtct ttcaagcggc ggaactccga tattaccgat
     1921 aagcatcgta tctcttccgc tcttctcaat aaagaattta ataaggcttg aagtagttga
     1981 ctttcccttt gttcccgtga taccgatcgt catattttcg caaaagcgta aaaacaagtc
     2041 ggtctgcgat gttatcttag cctttatttc atcgctcacg ctgtccttaa gacctatacc
     2101 gggagctttc attataatgt cgcagtcgtc aagacaggag agatagtttt caccgcagca
     2161 aagcgtacag ccctcggctt ccgctttgga aacagggttc atatcggcaa ccgtgatgct
     2221 tttgcaatta cagttcccga gcagcttcaa tgtagaacgt ccctctctgc caaatcctaa
     2281 aataacgacc ctcttgtttt caaaaaacgc tttcagcttt tcatctatag tcatatatcg
     2341 tcactttctt acctcagcag tccgataagg tgctgaggta caaaattatc attgtcaaaa
     2401 taattatcca tgctctgcgg tacgggcgga tcggtccttg cataacgcct gagaagatac
     2461 ggtaaattct caccc
//
LOCUS       DAWAOG010000306         2466 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5529_length_2466_cov_2.236831, whole genome shotgun sequence.
ACCESSION   DAWAOG010000306 DAWAOG010000000
VERSION     DAWAOG010000306.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5529_length_2466_cov_2.236831"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          534 a          647 c          531 g          754 t
ORIGIN      
        1 ccttatcgct tttctgagtt ctgactggca cattttaaaa acatattctc tgttactcaa
       61 taatgcagga tataggtacg gtctgggagg cgcaccatac gttgtatgat aattgccgtc
      121 cgcatcacgc caagtccacg gttgcatcgt gtgaggtaca cccggtgcgc cgagctgacc
      181 tgtgccgaac tctacaaaca tggcatattc aacgtttgta cctaccaccc aacatttaaa
      241 gccgccgcct gacacactgc cggtatattc gttcttttta ttgtcggcat agctgaaaga
      301 atgaaactcc tgatttgtac attcaatgct atttttcaat cttgcggtat cataaggaca
      361 gttcttttta gcgtcacgct ttatcttctg cgctcctctg cctaagccct tgttcatagc
      421 cgcatctacg cttccgccga gccgtctgag ctttgccatt aaactttcaa gtccttcgat
      481 tgatacttcc atgcttatgt cctttctgcc gttgcgctgt cgtgcatagt ataatgtgca
      541 acggagagta tcttataatc tgctccgtca caccttacta tatctccgac tttaagcgtg
      601 tctttatccg ttgttgctat cgtcagcata ccgtgtattc tctcgccgta cagttcgaca
      661 gatacgctgt cggttaccgg ctttacaaca gcagatattg tcgctacctg tttaagctcc
      721 gataccgttc ccacatagtc gcttttcttt gatgccttgc gatacaccgc aaatgttctt
      781 gtatcaaccg tcatcattcg cacggataac accgaccttt ctgggataat tctgtaatcg
      841 tttctgtaaa tcgggcggca gatcggatac aaaggaacgg gaaataccgc cctcgctgcg
      901 agcggtttct ccctctgctc cctgtctgtt atatgctata accgcaagct ctgtctgcac
      961 gctgacaagc cttgcaggca gttcatctct gccgataaca tcaaggatag tgtcctctgc
     1021 gctgtcaaga agcacggcaa gcagtccgtc ctgcttttca tcggaaatac caagacggat
     1081 tttaagcgtt tccagtgctg tcattgcgtt tctccttacg atgttgactt aggcagtatc
     1141 ttaatgccgg tcagcacacc tgcttttttg gtgttcttga gcgcaacacc ggcgataagc
     1201 tcaacgtcac ctttctttac cgctccggga gctgtgaggt caggcatata agaggatatt
     1261 acctttgtac ctgtaggcga aataccgtgg aaagcgtcaa gacctatctt tattgcgtaa
     1321 atatccgttg tgccgtaagc ggtagttgaa ggcgttgttg tatcaacgat atctaccgaa
     1381 gccgtaccat tgtagtattc gccacagtca agcagtgcta taccgttgta atattcaaca
     1441 ggagtaccga atgaatcttc gtttctgctg taatagcctg ctctccttgc acacgctctg
     1501 agctttgcca gcatttttcc gttcataagg agcatatcgg gcttgccgtc aagcagtgaa
     1561 aggaagctgt caagctcatc gaggaacgca ttatagttag tatcggtaag cgccgatgtt
     1621 gaaagatccg ccattgacgt tacctttgtg tctgctgttg aaagtatctt ctttaatccg
     1681 tcaaacgtgc ctgttacata gcctgcgccc gaagcggcag atgtgccgtt aataacaagg
     1741 ttcgtaaaat aattggacgt tgcctttatc ttctgctctg cctggaatgc gagctcatca
     1801 actgcgccgc ttgtgttggc tataactctg tcaacctcaa acgaaccacc catgattacc
     1861 gcttccgtcg ttttcttttc tctctttgct tcgcttgccg tgtactcact gtttatggta
     1921 cgcaccgaag cggtagcagg tgtttttagc tgtacatatc cgtatgacag cgttgaaccg
     1981 ttagtgccgg gtgaaattga attgtcaaat acaagtctgt caaggagaag gcttgaacgt
     2041 ctgaatgtgt cgattacctg ctgatccacc ttatcagcca taccgacttt tgcttctgcg
     2101 agtgtgattg ccataatttt ttacctcttt ctgattattt tcctgcctgc ttcattctca
     2161 gggcttcggc aagagaggca gggttagttt ttcccttgcc cgatgcgcct attttcggcg
     2221 gattgccctt cattctttcg ttgaccgcgc attcaacggc ttcggcaaat gccttgctga
     2281 ccgtttcaat gctcgtcttg cattcatccg caccggtata gtcaagcaca gcggcaagcc
     2341 ctacgggaag tcccttatcc gcaagctgta ccttagcttc cgccataagc tcacgccttg
     2401 taacctccgc ctcacgcttt gcaagctttt cctcagtctg ttcgcgctga tactcggctt
     2461 tctgct
//
LOCUS       DAWAOG010000307         2462 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5534_length_2462_cov_2.454508, whole genome shotgun sequence.
ACCESSION   DAWAOG010000307 DAWAOG010000000
VERSION     DAWAOG010000307.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5534_length_2462_cov_2.454508"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          660 a          564 c          500 g          738 t
ORIGIN      
        1 aaactgtttt cagcgttgtt ggctgtatat gttgctctga cttttttaca atatgttgca
       61 ttgtaatttc aatgtaatgt gccacagcca ctatttattt aagcttttaa attcagcatt
      121 accgacgaca taaacatacc gtcctttatc tccgagcgat aagtaccgcc gtatttttcc
      181 gctatgctct tgaccgactc gacaccgatg ccgaaaccga tatgctttga agaaaacaat
      241 acaccgttga catccttttt tacgttaccc gtaaatgtgt tgtcgatacc gaggcaaagc
      301 gtatattcat cggtcactgc ccttatcact atctttctgt cgtcactttt ttcctttccg
      361 acggcatcta ccgcattttc gatcagattg ccgaacagta ccgacaggtc tgtttcatca
      421 acagctattt tttccgggat gacagccttt acatcataat ctatatcttc gttttttgcc
      481 atctgtgcga aatacagcat tacggcattt acagccttgt tttcacagaa atttatcagc
      541 gtatcatcgg gcagactctg ctgatagctg ttcagatact tttcaagctc atcatattca
      601 cgatttctca catactcctg cataagcgaa atatgatgac gcacatcgtg ctttgcccgt
      661 cttgcatctg ctatcttatc ctgcaggttt tcgtattgca ggttctgcat tgcaaggcga
      721 tgattttttt cactgagcct tatgtttttc tcctgctcat ttatcagatt tgctatgaca
      781 taataaacaa ggcaggcgcc gatatttata aagaacagga atatcgcatt accgacatca
      841 agcgcagttt caaggctcga ctttccgttg ttattataaa gcacatagta ccatatcaga
      901 tagaaggtag cgggtataag caacaaatat cgccactcga tacctgtcgg ttctttttcg
      961 acagccggag tatatacttt cttagtgtag atgaaaagcg gtatccacac caccaccaac
     1021 acgatcagca ttataagcga gaacgaccaa cggtacgact gcaatgcaag gtcggggaaa
     1081 aatatgcctt cgacacactt tgatgcagcc acaacgaaat ttgccgtatt ggatatcata
     1141 agcagagtga atacagcctt tccgaaataa actttcacgg ctataaaata gaacactgta
     1201 taaagcaccg tgctgacagc actggcaagc gccgcctttc ctcccgagaa gaaagccgcc
     1261 caaagtccga gcccgatctg tacaaatgtc ataagcacta taagaaagac tgtaactctc
     1321 tttgaaaaac gcagtcttga gcggaacggg taaagcgcca gtgccataaa cggcagaaag
     1381 ttaagcagtg aatatatgca catctctgct atccggtata acggaggcat cagcactcac
     1441 cgcctttcct gagcttttca aagcaatagg aggtgtaaat gtcaaccgct tcttttgcct
     1501 ttcttctgct tatcggaatg cgggtatcgt cagacataag gaaaatatca ttcttttgtg
     1561 ccgacacttc attgagattt atcactatgc cttttgacgg gctgaaaaag ccggggtatt
     1621 cgcacaacgt cgcttcgaca tttctgaaag cggttctgag cttcacgtca ggactgtttg
     1681 tgtaatgtat attgacgcag tgtgccgcaa aatcggcata tacgatattt ctgagaatta
     1741 tcgcagtacc gtccgggagc attgctgtgc gccgtttatc cacttcatca agcttaagcc
     1801 gttcaagcat tatttttatc tgatcttcgc cgtaaggctt gtgcagatag tagcttgcat
     1861 tgacctcata gctttctccg gcaaattcgt tgctggcagt agcaaagact atgcggacat
     1921 ctttatcatc gtttcttatg atacgcgcaa tttccatacc gttcttttcg cctatgaata
     1981 tatcaagtat gacaacgtca tatctgctct ttgcccacgc gtctaaaaac tcctcgccgc
     2041 tgtggaagca atgcgcttcg acattgctca gattgttatt tatatatgac aggaacgtat
     2101 ttatttcgcg ctcgtcatcg tctgctattg caaatctcaa cttataaccc cctgtctttt
     2161 tttgatggct gtatctataa tagcacaaaa gtgagaattt tacaataaaa acagcggctt
     2221 gtgatgaaaa aacaggggtt gtgacgcaac ccggaaaagg aaaggtgagt cgaagcgcaa
     2281 aggatagtgc gcacggctca cttttttgta tggtttgctc ctattatatt gctttagcaa
     2341 ttaactttga tagcaggcta ccgatagttc tgtttgtggg gtagcacttc tcgggttcgc
     2401 tattctatgc gataacgttg ctgctatgtt tgagggagac aacccaaggg gagagaggaa
     2461 gg
//
LOCUS       DAWAOG010000308         2440 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5588_length_2440_cov_2.367715, whole genome shotgun sequence.
ACCESSION   DAWAOG010000308 DAWAOG010000000
VERSION     DAWAOG010000308.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5588_length_2440_cov_2.367715"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          773 a          516 c          600 g          551 t
ORIGIN      
        1 agcaaaattc aatgaatgga ggaagcgact gtgagagaag aaaagttcca tatcgcaaaa
       61 gagcaccttg taccgaagca gctcaaaatg cacggcatca agtcgcaggt gcgctttgca
      121 aatgacgcat tatatactat aatagacttc tacacgaagg aagccggcgt aagaaagctt
      181 gaaagaaaca ttgctaaaat ctgccgtatg acgctcaaaa agctcgcatc cggtgaagca
      241 gaaaagataa atatcaaggc aaaggatatc gaaagctacc tcggagcaag gaaatataca
      301 ggtgaagcca tttcaaagca agacgaggtc ggtgctgtaa acggacttgc atggacttcg
      361 gtcggaggta cgcttttacc gcttgaagta ctggtgatgg aaggaacagg caagattgag
      421 cttaccggct cactcggcga tgtaatgaaa gaatctgcaa agatcgcagt cagtctgaca
      481 cgttcgcttt cacgcaagta tgagatagac ccggagttct acaagaacaa ggatatccat
      541 atccacgcgc ccgagggtgc agtgcccaag gacggtcctt ccgcaggcgt aactatgaca
      601 acggctcttg tttctgcgct ttccggtatt gccgttcgca gagatgttgc aatgaccggt
      661 gagataacac tgcgcggtaa ggttctgcct atcggcggac tgcgtgaaaa aacgatggcg
      721 gcatattcag ccggagtaaa gactgtagtt atccccgatg aaaacaaagc tgatatgaaa
      781 gaacttgatg atgtcatact gaacaacata aactttgtac ttgccgacaa cattgacaca
      841 gtgctgaatg cggcactcgt aaagccgaac gttaccgccg ccaagaaaag tgccgacgat
      901 ctggcatcgg caaagccgcg cacatcaaga aagacaagca agagcatcat aaaggaaata
      961 tgatatgaat tttaataagg ctgagtttta tacatcctac ggtctgttta aacagatacc
     1021 gaaatccgaa aaagctgaga tcgctttttc aggaagaagc aatgtaggaa aatcctcgct
     1081 gataaacaag atacttaaca ggaagtcact cgcccgtgtc agctctatgc ccggcaagac
     1141 tgtgaccatc aatttctact cggtagacaa catatacttt gcggatctgc ccggttacgg
     1201 ctatgcaaag gtatcaaagt cggaaaaaca acgctgggca ggactggtgg agggttatct
     1261 tgcagatgac agaaaccttg cgcttgtatt ccagctggta gacttcagac acccgccgac
     1321 agctgacgat ataatgatga taaacttcct tatcgacaat gagataccgt ttgttgtcgt
     1381 tcttacaaag gcagacaagc tgactaaaaa cgagcgtgca aagcgcagga aggctctgct
     1441 ctccgagatg ccgtgtgcgg aggatatcca tatgatcgaa ttttcagcta tgaacggcga
     1501 gggtgtggaa gagatccgtg aaataatcga agatattgaa caggaagccc ttgaaggcaa
     1561 cgctcccgat gaagcaggca aagagcagtg acggacatat attcgctgac atatgaacaa
     1621 gcggagcgcc tgctcaccga aaacggcttc agggcaacac agtgcggcaa catattccgc
     1681 gacatatata agcgcagagc gacaagcttt gatgaaatga cgctgacatc agccgatata
     1741 aaagcgcttt tatccgactg ttttttcttc ggaacgttga aaatcgacga aatattacag
     1801 tcagtagata ccattaagta tctttttgaa ctgtcggacg gctgtaaaat tgaaactgtg
     1861 cttatgcgtc agaaatacgg caacagtatc tgtatctcaa cgcagagcgg ctgcaatatg
     1921 ggctgtaggt tctgttgcag cggaaagctc agaaagcagc gtgatcttac cgcaggagag
     1981 atgataaatc agatacttat tgtcgaaaaa catcagaata taacgatcag taatataaca
     2041 gtaatgggta taggtgagcc gtttgacaat tttaatgcac tgtgtgactt tattgatata
     2101 gtaactgttc cggttggaat ggaaatagga acaaagcata tcaccgtttc aacctgcgga
     2161 ctgtgcgaca agatgaaaat gtttgccgag cgcaaagacc catgcaatct tgcggtaagc
     2221 ctgcacgctc ctgatgatga gataagaaac aggcttatgc cgataaaccg caggtatagt
     2281 atctcacaag tcatagaatc ggctaaatac tatgtcgaac gtacaaaccg caaggttctg
     2341 cttgaatata tactgcttga cggcataaac gacagcaggg agaatgcacg tcagttagcg
     2401 gcgcttatcg gaaatgcacg gctgtttgta aatcttatac
//
LOCUS       DAWAOG010000309         2438 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5596_length_2438_cov_2.275283, whole genome shotgun sequence.
ACCESSION   DAWAOG010000309 DAWAOG010000000
VERSION     DAWAOG010000309.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5596_length_2438_cov_2.275283"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1834..1843
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          740 a          542 c          504 g          642 t
ORIGIN      
        1 gtcctatctt cctgtgtccc ttgcggataa ggtaatttac cgcgtcgcga cccgccttga
       61 cattatcaat tgtaacggta agaacattac agttatcaag acgttcaagg cacattgcga
      121 tattgtgctt ttcgccaagc gcattgatag tagctgcatc aagcttaggt cccaggagca
      181 cagcaccgtc taccgccctt gaaaacagca taccgagtaa gtgcatctcg tgatttggat
      241 catcatgagt tgtagctatc aatacatcat aaccctgcga gaaagcggca acctccatac
      301 ctctgataac atcggaatag aagctctgct ctgtcgatgc gattatcgcg agtattcttc
      361 tcgtttcgct ctttctgaga tctctgccga gaaagctcgg gctgtagccg agcgcctgta
      421 cagcactgtt taccgcctga atagcctctt ctgacacatt ggacttttta ttaagaactc
      481 tggaaaccgt tgctaccgaa acgtttgcag ccttagcaac atcctttata gttacgctca
      541 tttatcggta ctccccctta aagatacact atattacaat tatacagtat cgtgaaaccg
      601 ttttcaatgt tacatttcaa caaaaaaatg tatgaaaatt tatcgaatat tacaatttgt
      661 cgatttttac ttcatttact cctacaattt gctgtgataa aagatgctga ttttctccta
      721 aatttagcta aatttttaat acttccgctt aaacgaatga aaacaaaccg ttacatttat
      781 aactttagct aaaattataa agtaaacaca aagaaatgct tgttttttgt gcaaaaagtt
      841 actttacaaa tcggcggttt tagtttacac tataataaaa ggaacgatat aaagttatta
      901 acttaaacaa actgcgcaga ggtacttgaa atgaaaaaga aatcagttac aatgagtgac
      961 attgcaaaga ctatggacat cagcaccgtt actgtgtcga aagcactcgg agataaagac
     1021 ggtgtgagcg aggagctcag agagaagata aaacagaaag ctaacgaaat gggctaccgt
     1081 ttcagctata catcaaaatc gtcaaaagag ggctttacgt tcaatataag tatagtagtt
     1141 gcaaagcact ttataaatga cgcgagtgca ttctactggg taatgtaccg tcatatagta
     1201 gagcttttac aaaagcagaa ctactacggc atacttgatg taatttccga aaacgatcag
     1261 gcaaaccgtt caatgcccaa ttcggtatcg gataaaaagg ttgacggcgt tattgtgctc
     1321 ggacagtttg atgatgaata tgtcagcgag cttcttgatc tcgatatacc ggttgtattc
     1381 cttgactttt acagcagcga caaccgcacc gatactatcc tttctgacag cttcttcggc
     1441 tcatatatgc tgacaaacta tctcataaat gcgggtcacc gcaacatagg cttcctcggc
     1501 actatcacat caacatcaag catacaggac agatacctcg gatattacaa ggctctgctt
     1561 gaaaaaggca taccgctccg cagtgaatgg gtaatagacg acagaggacc tgagggtact
     1621 agctatccgc acttcgacct gcctgcacaa aagcccaccg cttttgtctg caactgtgac
     1681 gaggcggctt actgcctgtt aaatcagctc aggagcgagg gctatgtagt gcccgatgat
     1741 atttcgatag tcagctacga caaccacatc tactcgacta tctcaaatcc ccgtatcaca
     1801 acaatagacg ttgacagcaa gagaatgtca tacnnnnnnn nnntaagtga taagaactat
     1861 tcgcgtggca gaacgcttgt aacaggcaag ctgattatcc gcgacagtgt aaagatattg
     1921 aagtaatgaa aattatagat actcacgcgc actatgacga cagctggttt gacgaggaca
     1981 ggtacgaggt tcttgaccgc atactgtcgg acaatgtgct tgcaattata aatatgagcg
     2041 tggatatccc cacctgcgac ttttcgctga ggcttaccga gcgttacgaa aacgtgttct
     2101 gcgcagtggg aatacacccc gaaaacatag ccggcactcc cgaaaattat attgatattc
     2161 tgagggagct tgcaaaaaat aaaaaggcgg tagccatagg tgagatcgga cttgattatc
     2221 actatgaggg ctatgacgca caaaaacagc aggagatatt tgaaaatcag ataaagcttg
     2281 cgaatgagct ttcgctcccg gtcgttatcc actgccgtga cgctacggag gatacgatga
     2341 aaattctccg ccgcaccacc cccgataaat tcgtggtcca ctgctactcg ggcagtccgg
     2401 aaaccgcaaa ggagctttta aagctcggcg cgtatatc
//
LOCUS       DAWAOG010000310         2427 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5625_length_2427_cov_1.937184, whole genome shotgun sequence.
ACCESSION   DAWAOG010000310 DAWAOG010000000
VERSION     DAWAOG010000310.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5625_length_2427_cov_1.937184"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    446..455
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1789..1798
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          654 a          534 c          517 g          702 t
ORIGIN      
        1 gtttctatag caagcggaag ctctgttata aaagacagtt ccccgtcgaa tttctcgttc
       61 acagcattac gcttgttatc aaaatctgtc gtgaaatcgg taataccgat actctccgcc
      121 atttgcatat aagcgtttac agggcggtat gtaagccttt cgagaaactt ttccttttca
      181 tcgtcaaacg ccatacgcga taccatcgta tcgttgttac ccttgataca tacgcatttt
      241 ttgtcgcggg ataatttttg tataaagtgc agtgtttcga tattttgcct gcccttttcc
      301 agaatatcac cgagaatgaa cagatagtcg ctttcattat tgtagtcgca cttacgcaaa
      361 agcttcgcaa actcatcata atgtgcgtgt atatcactga cgcaaattat cctgcacttt
      421 tcaggcagat ctattttcct tatgtnnnnn nnnnnttgat catattatca tcatactgtc
      481 gccgaagctg aagaaacgat atttctcctg tatcgcaaca ttataagcgt tgagcgtctt
      541 ttccctgcct aagaatgcgc ttacaagcat tataagcgta ctttcgggaa ggtggaaatt
      601 tgttatcagt ccgtctatag ccttgaactc atagccgggg taaatgaata ttcccgtatc
      661 gtgcgagcag gcttttatct cgccgttcgg gtcaagcgat gcggcagatt cgagtgtacg
      721 acagcttgtt gtgccgactg aaataacgcg tcctccgcgt tctctgcaag cctttatctt
      781 atcggctgtt tcctgcggta tcgagtaatg ctcaacgtgc attttatgat cgagtatatt
      841 atcctctttt acagggcgga atgtgcccag tccgacatga agagttacaa atgccgtatc
      901 aacgcctttt tcgctaagct ttgtaaacac gttttcagta aaatgaagac ctgctgtcgg
      961 tgcggcggca gagcctacct catgggaata gatggtgttg tatctgtcct tatcttttag
     1021 cttttctgtg atataaggag ggagaggcat ctgacctata cggtcaagca cttcaaagat
     1081 gttatcaccg tcataggtaa actcggctat cctgtcgccg ccctcggcat aatcgactat
     1141 ctcggctgta agtattcccg aaaagtcgac cttcgtgccg attttaagtc ttttgccggg
     1201 tttgaccata acctcccagc gattatgtga tatctgatgc aggagcagaa actgcacagc
     1261 ggcacccgtt tcctgcttta cgccgtaaag ccttgcggga aagaccttgg agtcgtttaa
     1321 aacgagcaag tcaccctttt taagatagtc gcatatatta taaaagcggt catgtgttat
     1381 ttcgccgctt atgcggtcaa ttttcataag ccgtgagctg tcacgcggtt caagcggagt
     1441 ctgcgcgata agctcctgcg gcaagtcata ataaaaatcc gatctaagca ttgtttttcc
     1501 tctgtttttg cgtttttcac gcactttttc tttctgaata ttgcatactg ttttataata
     1561 acacatttac ggaaaaaaat caaattattt tttaccgttc cgccattgac aaacagcttt
     1621 aaatatgata atataaaaaa agcaggtaga ggtgcgatta aagaagagta cccgttgcgt
     1681 caatctgccg acagattgca gcgggaaaag gctttgtcgc cgaagaacat aagtgtcgga
     1741 gcttatgtat ctggtcgtgt gtctaatata tgcacgattg tcatcaagnn nnnnnnnngc
     1801 tgtaggtgga gagctatcac acaactttat cacatctgca tacaaagccg cctgtaatca
     1861 acttacaagc ggcttttttt aaagaaatat gctataagca aaaaagaagg atggaaacta
     1921 aaatgaaaaa ctacaatgta ggtattatcg gcgctacggg tatggtagga cagcgttttg
     1981 ccgttctgct cgaaaatcat ccctggttca acgtaaaggt gcttgccgca tctccccgtt
     2041 cggcaggaaa gacttataaa gaggctgtcg gctcaagatg gctcatcgaa aagcccatgc
     2101 ctaaagctat gtaagatatc gttataatgg atgctactgc cgatatcgaa aagatcgcat
     2161 cacaggtcga cttcgtattc tgcgcagtag atatgaaaaa ggacgagata aaggctcttg
     2221 aagaggctta cgcaaagcac gagtgccctg ttatgtcaaa caactcagct aaccgtttca
     2281 cagacgatgt tcctatgatt atccccgaga tcaatgacga gcatatggct atcgttgagg
     2341 ctcagaaaaa gcgtcttggc acaaagagag gtttcatctc cgtcaagtca aactgctcta
     2401 tacagagcta tatacccgca ctcaacc
//
LOCUS       DAWAOG010000311         2412 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5673_length_2412_cov_2.507425, whole genome shotgun sequence.
ACCESSION   DAWAOG010000311 DAWAOG010000000
VERSION     DAWAOG010000311.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5673_length_2412_cov_2.507425"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1570..1579
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          701 a          556 c          589 g          556 t
ORIGIN      
        1 ggggaagggg gatggggtta ggaatattcc tatgaaactt catttataaa gttgatgaca
       61 tttctgaggg cggatattct ccgcccttat tgttgcgtaa taagcaattt ataataaaaa
      121 acgaaaggaa cactgtatca tgaaaaagac agtaagaaaa gcagttatcg caggtaactg
      181 gaagatgaat aagacaagac ccgaggctaa ggctctttta gaagaattaa agcccatggt
      241 tgccgatgtt aaggacgttg aggtcgtagc ctgcgttccc tttacaaacc ttgaaacagc
      301 tcttgctgta acagcaggca caaacataaa gatcggcgca gagaactgcc actttgaaaa
      361 gagcggcgct ttcacaggcg aaatttcagc tgatatgctc gctgaaatgg gcgttgaata
      421 tgttgtactc ggtcactccg agcgtcgtca gtactttgct gaaacagacg aaacagtaaa
      481 caagagaaca aaggctgctc ttgaagcagg cttaaagccc atcgtatgcg taggcgagct
      541 tctctgggag cgcgaatgca acattacgga agaggttatc gcacgtcaga taaagctcga
      601 tttcttcggc atctcagcag acgacctcaa gaagtgcata atcgcttacg aacccgtttg
      661 ggctatcgga acaggcaaga cagctacagc agatcaggct gaagaggttt gcgcatttat
      721 ccgcgctaca ctcgctaagc tctatggcgc agatgttgca gaaacaatca caatccagta
      781 cggcggctct atgaacgcaa agaacgctga agaacttctc tcaaagacaa acgttgacgg
      841 cggtcttatc ggcggtgctt cactcaaggc tgctgatttc acaactatca tcaaggctgc
      901 cgtaaacggt taatcaaaaa tcggatattt acgaaaggac gactaaacaa atggcaaaac
      961 ccgttttact cgttgttatg gacggtgtag gcttttcaaa gacaggtctt ggcgacgcag
     1021 tcacactcgc caatactcct acacttgaca gacttttaag agaatgcccc aacacccgct
     1081 taaaggctca cggtgacgct gtaggtctgc ctacagatga tgatatgggc aactcagagg
     1141 taggtcacaa cgcactcggc tgcggtcaga tctactcaca gggcgcaaag ctcgttaacg
     1201 aatctatcga aagcggaaag atgtatcagt ctgaggcttg gaaggaactc gttgacaact
     1261 gcaaggctaa caacagcacc ctgcacttca tcggtctttt atcggacggc aacgttcact
     1321 cgaatatcaa gcacctgttc aagatgctta cagaagctaa gaacgagggc atcaagaagg
     1381 caagagtaca cattctgctc gacggcagag atgtacccgc tacttctgct cccgtatata
     1441 tcgaacagct tgagaacttc ctcaaggagc ttaatgccga tggcgctttc gatggtaaga
     1501 tcgcaagcgg cggcggacgt atgaaggtta ctatggatcg ttatcaggct gactggccta
     1561 tggttgagcn nnnnnnnnng acagttcgca tcggctatgg aagctgttga aacctaccgt
     1621 aaggaaaatg acggcatcat cgaccaggat ctccctgcat tcgtaattgc cgagaacggc
     1681 gagcctgtag gtaagatagt tgacaaggac agtgttatcc tcttcaactt cagaggcgac
     1741 cgtgctatcg aactttcaat ggcatttgac gatgacgact tcactgcatt tgacagaggc
     1801 gcaaagcccg atgtatgctt cgcaggtatg ctgcagtatg acggcgactt aaagctcccc
     1861 gcacgcttcc ttgtaaatcc tcccgagatc acaaacactc tcacagaggt actggttgcc
     1921 gcaggtctta acgaatatgc agtatccgaa acacagaagt acggtcatgt aacttacttc
     1981 tggaacggta acaagagcga taagttcagc gaagagcttg aaacatacaa ggagatcccc
     2041 tctgataacg tttcgttcga tcagcgtccc tggatgaagt ccgccgagat aacagacgat
     2101 cttatcgagg ttatcaagag caagaagtat gactttatcc gttgcaacta ccccaacggt
     2161 gatatggtcg gacacacagg cagccttgac tctacaatta tcggtgttga agctgttgac
     2221 ctcggccttt caagacttat caaggtttgt gatgagtacg gtgttacact cgttgtaaca
     2281 gccgaccacg gtaacgcaga cgagatgctt gaaaagaaca agaagggtga ggttcaggta
     2341 agaaccgctc actcgctcaa ccccgttcct ttcatcatct atgataagga cgttaagtac
     2401 actatcaagg ac
//
LOCUS       DAWAOG010000312         2412 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5671_length_2412_cov_3.002546, whole genome shotgun sequence.
ACCESSION   DAWAOG010000312 DAWAOG010000000
VERSION     DAWAOG010000312.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5671_length_2412_cov_3.002546"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          567 a          683 c          491 g          671 t
ORIGIN      
        1 cccgccggta acgcaactgt cgcctatcgg caacaatcag aacggcgaat ttgaagcggc
       61 tcacagccgc acggcgaatt tacagtggct ttcagccaca agcggcacat tgccgccacc
      121 cgcttactta gtaccgaaca gtctgtcgcc cgcgtcacct acacccggag tgatgtaaag
      181 atcctcgtta agtccgtcat caagcgcacc gcagtagatc tcaacatcgg gaaaagcctt
      241 ctgtatagcg gcgataccct cgggagcggc gataatgcac ataaacttta tagaacgtgc
      301 gccggcttcc tttaccttct gtatagcgtg tactgccgat gtacctgtag caagcatgat
      361 atctgtgatg attacgtcac gctcgtcaat atcgaacggg agcttgcagt aatactcatt
      421 tgcaacagtt ttgtccgatc cgccttttcc tctgaatata ccgatatgac ctatcttagc
      481 ggcaggaaca agcgcggtag caccctcgat cataccaaga cctgcacgga gaataggcac
      541 aaatgcaagc tttctgcccg aaataacgtt agactctgca atagcaatag gtgtctgtat
      601 ctgtaccttc ttcaggggca gatctcttgt tgcctcatag cacataagca ttgaaatttc
      661 gcttacaagc tctctgaatt cctttgcgcc cgtgttctta tctcttaaaa gagatacctt
      721 gtgctgtaca agggggtggt cacagatgtg aagtttctcg tttgccataa catactcttt
      781 tctccctatg ggtaaatgga tttttatttt gttagtcctg ccgtgatttt catagggaaa
      841 aatccgcagt ttttaaatac ttacttattc tcaagcgcca tcatcttgtc aactcttgcg
      901 gcgtgtcttc cgccctcaaa ctctgttgac aggaacactc ttactatctc ctcagccgta
      961 cccggtccta tgaccctgcc gcccatgcac agaacatttg cgtcattgtg cagacgtgtg
     1021 tacttagccg tataataatc ctggcataaa gccgcacgga taccctttat cttgtttgcc
     1081 gatatactga tacctattcc ggtaccgcat atcagaatgc ccttgtcgca ttctccgctt
     1141 gtgatcttcg cgcaggtctt ttccgcaata tcgggataat ccgctctctc tccgtcacag
     1201 ccgcagtcga tatactcgac cttaagctca tcaaggaact tcttaacctc gcacttcagt
     1261 tcatatcctg cgtggtcgct acctattgcc agcattttta ttctttcctt tctttatatg
     1321 cctcagcatt ctttgccata agctcacgct gagcctgcgg cagcttaaga tacatcggca
     1381 taagctgttc gggcgttaac tgcggcgcat caacagccgc aaggcacact ccgtagcccg
     1441 tctgatatct taacggatcg ggggcgatct ttacattttc cattccctcg ttatccgttg
     1501 cttttttcat tataaatgcg ccgtcaccga caagcattat atcatcgtca tattccgaca
     1561 gcattcttgc cgcttcgtcc gtcttaaggc actcttcctc tgtcagcctt tcaaccttgc
     1621 cgtcctcaat gcggaacagc gcaacgtaca cctgattgca ccttgcgtct attgcggagc
     1681 atacaacaca gtcgtaagcc gtaacgttat acgccatagc ttcaagtgtt gaaaccgccg
     1741 cgcacggctt tgacaatgca aaagcaagcc cctttaccgc cgatatgcct atacgcagtc
     1801 ccgtgaaaga tcccggaccg ttcgccactg caaaaagccc tatatcctcc atagaaagct
     1861 ccgcatttct gacaagatcc cttacaacag gcatcagcgt aatgctgtgc gtaagcttgt
     1921 tgtttatcac cgtactgccg agtatctgct gcttttccgt atcatacacg gcggcacagc
     1981 aaggagcggc ggcggtgtcg ataccgagta tcagcatagt tcctcctgcg ggatatattc
     2041 tattattctt gaattatccc ctgtcttggt gatattcaca ccgcacctgc cgtcaagata
     2101 cgaagcaagc tccggtatgt tctcgctcca ctctatacac attatcccac ccctgtcgag
     2161 gtagtcaaaa aatccgatag cgtacagatc gtcaagcgtg tttattctga aaaggtcaaa
     2221 gtggaagatc ggcacatcgc cctcatattc gttcacaaga gcaaacgtcg ggcttgtgac
     2281 atcgtctgta ctgccaaaat attccgctat accctttgtt aaatgcgtct ttcccgcacc
     2341 catttctccg gtatacagca cacagtcgcc tgcttttaag tatgcggcta tccttttgcc
     2401 gatctcaacc gt
//
LOCUS       DAWAOG010000313         2410 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5682_length_2410_cov_1.765605, whole genome shotgun sequence.
ACCESSION   DAWAOG010000313 DAWAOG010000000
VERSION     DAWAOG010000313.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5682_length_2410_cov_1.765605"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1177..1186
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          708 a          510 c          577 g          605 t
ORIGIN      
        1 caaattctct acggcatcac tgattacgag aagcacgaac gatatacagc agatacagat
       61 ggtcatcgta atgcttttga gaatggttat ctatgcgccc atactcggca ttggcggcgt
      121 gtttatggtg ctgactacag gtacagatat ggcgtggata atcgcacttg cggttattgt
      181 tatacttgcc gttgtacttg tgcttatgaa gatagctatg cctaagttca agcttatgca
      241 gaagctggtg gacaagctga accttgtagc aagagaaatt ctcacgggtc ttcccgtgat
      301 ccgtgctttc agccgcgaaa ggtatgaaga gggacgtttc gataaggcta ataaggatct
      361 gacatcaaca atgctgttca caaaccgcac aatgaccttt atgatgccgc ttatgatgat
      421 gataatgaac cttatcacgg ttcttatcat atggataggt gccggtcaga tatcggacgg
      481 ttcactccag gtaggcgata tgatggcgtt tattacatac acaatgcaga tagtaatgtc
      541 atttttaatg ctgacgatga tatcaataat gctcccgaga gccgcagttt cggcagacag
      601 aataaatgaa gtcataacgg caaagaagag catagaagat aaaccgggtg caggaacttt
      661 tgataaatcc acactcaagg gcaatgtatc ctttgaaaac gtatcattcc gctatcccga
      721 tgcagatgaa gatgtgctga cggatatcag ctttaccgcg aatgcaggtg aaaccaccgc
      781 tattatcggc agcaccggca gcggcaaatc aacgcttatc aaccttatcc cgaggtcgtt
      841 tgacgttacc aaaggcagga ttgcaatcga cggtactgac ataagagatg tgacacagca
      901 ttctcttcac gaggtgatcg gtcttgtgcc gcagaagggt gtattgttct caggcactat
      961 cgacagcaat attaagttcg gcgcggaaaa tgcaagcgaa aaaacagtga agctcgccgc
     1021 cgacgtttcg cagtcgtctg aatttattga cagcaagccg caaaaataca acgaacctgt
     1081 ttctcagggc ggtactaacg tttcaggcgg acagaaacag cgtctgtcaa ttgcccgtgc
     1141 aatagcaaaa gaaccgaaga tatacctgtt tgacgannnn nnnnnnaagc tcagaaaagc
     1201 gcttgccgac aatattcacg actgcacgat aataatcgtt gcacagcgca taagcacgat
     1261 tcttaacgca gataagatag tagtgctcga tgagggtaaa atagccggta taggaactca
     1321 cgagcagctt atgcaaagct gtgaggttta caggcagata gctcactcgc agttatcaca
     1381 aaaagatctg gcggcaatag acaatgcaaa gggggttacc gacaatgagt aattctcaca
     1441 gaagaggccc cggtgctcca gtggaaaaga cctaagctga tgggtaatgc cacaaccgaa
     1501 atatttgacg gcattatcgc taaggtgaac ggcacgggtg atgtgaattt cgatgcactc
     1561 ggttcaatac tgcttcttct tataggcttc tatctcatca gcagtatctg ttcatttatt
     1621 cagggctggc tgatgaccgg aatttcacag agggtaagct acagactgcg taaggagctt
     1681 atcgcgaaaa taaaccgtct gcctatgaat tattttgatc gcagtactca cggcgaagcg
     1741 ctttcaagag taacaaacga tgttgatact ctggcgcaga acctgaacca gagcataaca
     1801 cagctcgtta catctgtaac aactattatc ggcgtactca ttatgatgct gtcgataagt
     1861 ccgcttatga cgcttgttgc gcttctgata ttgccgatat cacttatact cgtactgatc
     1921 gttgtaaaga tgtcgcagaa gtattttagg tcacagcagg aatatctcgg tcatatcaac
     1981 ggacagatcg aagaagtata cggcggtcat aatataataa aggcattcaa cagagaagag
     2041 cgtgttataa aggactttga cgaagcaaac gatgttttat acaagtcagc atggaaatca
     2101 cagttcttct caggcataat gatgccggta atgacctttg tcggaaatct cggatatgtg
     2161 ggagttgcgg ttctcggcgg tttcctcgcc gcaaacggga ctattatggt cggcgatatc
     2221 ctgtcgttta tccagtatgt aaagaacttt acacagccga taactcagct tgcacaggta
     2281 tcaagtatgc tccagtccac tgtggctgca gcggaacgtg tgtttgagtt tctttccgaa
     2341 aacgaagagg tacagacggc aaagcaacac gcaatgctta ccgatacatc gggtaatatc
     2401 agaaatgtaa
//
LOCUS       DAWAOG010000314         2407 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5690_length_2407_cov_1.555697, whole genome shotgun sequence.
ACCESSION   DAWAOG010000314 DAWAOG010000000
VERSION     DAWAOG010000314.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5690_length_2407_cov_1.555697"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    264..273
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          678 a          548 c          510 g          661 t
ORIGIN      
        1 gtagaatctg tgttgaactt gtagacaaag atgccgagtg ccgcagttat aatgaacagt
       61 attactgaag cagcagcaga ataaccgtag ttaggctgtg cagcgtggaa gttctgataa
      121 atgaataccg cggtcgtctt taagctctgt cttactacag taccctcatg taacagataa
      181 ggaatatcga acatctgaag accacctata agtgatgtta taacaacata aataaagata
      241 ggcttcagaa tcggcattgt tatnnnnnnn nnnaagaatg tatgcagtcc gcttgaaccg
      301 tctatcgaag cggcttcata aatagaagca tcgataccgt aaataccgga cataagaaga
      361 agcattgtat taccgtacca catccatgac tgaatgaaca taacaaggac tcttgatgcc
      421 catacattat tgataaacgg gaacgcttca tttacccagc cccagttaag taaaatagag
      481 tttattgcac catactttga gttaccgaat atcataagga acataccggc aacagaagct
      541 gcagtgataa tattaggcag atacataagt accttgaata cgcctgctcc cttgatctta
      601 actcttacat cagagaacca tactgccaac aacagtgaaa gcagcaactg aattactaag
      661 ttacctatcc agagaatagc tgtattgcct aaagtattca gaaaatcttg ctgaatagca
      721 gcaccggcgc taaattcacc gcctgccgca tcaccaaaca gaagatactg atagttctgc
      781 aatcctacga atgcatcggg gttaccgctg ttacctttta gacttaggat aaaggtattg
      841 ataagcggat aaatcataaa aaatgtgtaa accaagaaaa atggtaaaat aaatagatag
      901 ccatatttag catagcttac cattttatga ggcttaccgg cagttctctg cataaaatac
      961 acacctttca actgattttg gctaaaaaat ttacaatata cgcttatggg cgatagccca
     1021 taagcaatat attgtagaac catcaataaa ttgtattagg gttgaatgct attgattatt
     1081 caacagtgag gtcggggaat gaacccttaa cgagcttctt gaatgcattt atagcgtcat
     1141 cctttgtagc atatgtgcca tcgaggtaac cctgaactga tgtgttgaag tgacccttga
     1201 ttactgagtc atactttgtg atcataccat ccatcttgat gccttcagct gctgtgtaga
     1261 gaatagcgaa ctgatcctga ccatccttga ggagtaagtt cttgtttgtc tttgcttcag
     1321 cgatatcctt cataacagtc ttgttattgc agaagtcgcc tgtgaactct gtgtacttct
     1381 tcattgtatc agcatttgtt gtaaagaact caacgaactg cttagcaagt gacttgttgt
     1441 cgcactttgt tgaaacgccg agccatgtac cgccccagaa gtagtttgta ggacctgcac
     1501 agaatgccat ctcgtaagta ggctttactt ctgtaccttc gcctgtgccc ttagcaaagc
     1561 tgtaaaggat cgagcttgta cctgttgtaa gtccccaaat aatacggcga ccaccgagag
     1621 ctgtaccatc ctgaactgct gcgtaccaag catcctgcca ctgaggaacg tctgccatgt
     1681 acttgttgtc cttgaatgac ttagctatat catagaagtc aggagctgta tccatctcga
     1741 gcttgccgtc aactacccaa gcctttgttc tgttagcctg gtatacctgc cagagaccac
     1801 cctctgtagc ctgtaaagat gtctttccgc cgctcttatc atagagtgtc ttagctgtag
     1861 actggaatgt atcccagtcc ttaacgagtg cctgcatctc atcaggatta ttaacgccga
     1921 ggtatttctt agcgaggtct gctctgtaag caaaaccacc gggagctgcc tggaatgaag
     1981 cacccttgag aacgcctgca tcattcttac cgattgctac tgtgtagccg taagcatctg
     2041 aataagctga ttcttcaatg ccgagcgctg agagaggagc tgtcagctgg tcatcgttga
     2101 tgtactggag aatccagtca gcttcaagac acataagatc tgcatcgccg tcacccttga
     2161 ggtactgctg gtactggtca cgtccgcctt caccgtttga accctgcttg ctgatctcga
     2221 tcttgtcttc aggtgttcct gtttccttgc agaacagctt aaccatgtta acgatatcag
     2281 agtttgactc ccaagcgaga attgtcagct tgtcgccgtc atccttgagc atggagccca
     2341 tgagcgatgc acccgtgagc aggcggccca atgccgcgct gcaggtggca ctggtggtgt
     2401 gcaactg
//
LOCUS       DAWAOG010000315         2405 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5697_length_2405_cov_1.773191, whole genome shotgun sequence.
ACCESSION   DAWAOG010000315 DAWAOG010000000
VERSION     DAWAOG010000315.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5697_length_2405_cov_1.773191"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          598 a          626 c          527 g          654 t
ORIGIN      
        1 gtcctcaagc gtcaccgact cttcgccgag cgcagacgca agtgtttcca gtcctacagg
       61 acctccgtta tagccgttta ttatcatagt gaggaagcgc ttatcaaggc tgtcaagacc
      121 gagcttgtcg atctccagtc tgttgagcgc gatatctgcc atttcctttg tgatctttcc
      181 gtcacccata acctcggcaa agtcgcggac acgctttaaa aggcggtttg cgatacgcgg
      241 agttcctctt gaacgctttg ctatctccat agcgccgttt ttatcgcacg ggattgaaag
      301 tatcaccgac gaacgcatta ctatattgca aagctcctcg ggggtataca tttcaagacg
      361 cattataact ccgaacctgt cacgcagagg tcctgtaagc tgtcccgctc ttgttgtagc
      421 gccgataagc gtaaacctgt tcagatcgac ccttatcgac tgtgcagacg gaccttttcc
      481 tatcattata tcaagtacgc agtcctcaag cgcaggatat agtatttcct cgacctgtct
      541 tgaaagtcgg tgtatctcgt ctacgaacag tacatcgcct gtctgaagac ctgtaagcag
      601 tgccgcaagc tcgcccggct tttcgatagc gggacctgat gtcactttga aattaacgcc
      661 catctcattt gcgataatcc ccgcaagcgt tgttttaccg agtcccggag gaccgtacag
      721 caaaacatgg tcaaggctct catttcgtct gcgcgccgct tctatatata cctgaagatt
      781 ttcctttacc ttttcctgac cgatatattc gttcaatttt ttcggacgca aggaaaactc
      841 aacatcggta tcctcgacag aaaactgcgg ctcgacaatg cgcgctccct cgtcatcggc
      901 tatatgtgaa agatcgtttc tctcaatcat ttatacgctt tcccttcaaa gttaatgcgc
      961 ggacaattat cagaacttcg caagtttttt aagtccggcc ttgataagct catcggcaga
     1021 ggctgcgggg tcaagtccgc cgagcgccga ggtggcttcg ttgtttgtat aaccgagcac
     1081 cataagcgcg gatatcgcct ccgcaaatga atttgacggt gcggctacta tcggcactgc
     1141 actgcccctt acacctgcgg ctatttcctg ctttgatacc ttgtccttaa gctcaaggac
     1201 gatacgctct gcggtcttct tgccgatacc cggagcgttt gtcagcgcct tgctgtcgcc
     1261 ggacgctacg cacagcgcaa aagactgcgg tgtcataccc gataagatcg acagcgccgc
     1321 tttaggacct actcccgaca ccgagatcag cattttgaag cagttaagct ctgccgcatc
     1381 ataaaatccg aacaagtcta ccgcgtcctc ccttaccgca agataggtga gaagctttgc
     1441 cttttcgccg gttttaagct gtgataatgt attcgctgtc gtgcggcagg cataacctac
     1501 gccgccgcag tctatcaccg tgagattagg ctcgataaga tcgactgttc cgtttacact
     1561 gtatatcata atttactctt ttctatatat tcgatgttct gaggtttctg agcaatgaac
     1621 cgctggtctg accgtggcat atagtcattg ccagcgcatc tgccgtatca tcgggcttcg
     1681 gcactgcggg gagcttcagt atattcttcg tcatttcctg aacctgcttc tttaccgcct
     1741 gaccatagcc tacaacagcc tgctttacct gtaagggggt gtactcgttt atctcgatac
     1801 cacgctgtgc ggcagccata attataagtc ccctggcctg cgcaacgtca atgcctgtct
     1861 tctggttgtt agtgaagtac agccgctcga tcgaaagcgc gtccggcttc cagttatcaa
     1921 gtatctgaca gaaatcgtcg tatatctcac aaaggcggtt cgggaaaggc gtatccttat
     1981 ctgttgttat agctccatag tcaagcaccc tgaaactgcc tttgctgtaa tcgacgatcc
     2041 caaaaccgac tatcgcatag ccgggatcaa tgccaatgat gcgcatagcc gcctcctcaa
     2101 agtgattttg ctttatttgc agatgcatgt acagaaaaca aaaaacaaaa atccatacta
     2161 aagaagtata tcacaaacga aagaaaaatg caactttttt attagtcgaa aacgatgtga
     2221 tacaaattag caatacaaat taagtaaaat cggttgaaat ttttatttgt taggtatata
     2281 atagtgtaaa acgattggag aaaaaaatga gaaaaaaaca tctgaccgct gtcctgccat
     2341 tagctgtgct gtgtgccgct tttacagcag ggtgcagtgc aaagcaacca tacagacacg
     2401 acagt
//
LOCUS       DAWAOG010000316         2405 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5696_length_2405_cov_2.879149, whole genome shotgun sequence.
ACCESSION   DAWAOG010000316 DAWAOG010000000
VERSION     DAWAOG010000316.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5696_length_2405_cov_2.879149"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          666 a          525 c          532 g          682 t
ORIGIN      
        1 gaaggtagac gaggacggcg acacagcaat aggcacaaat gtactgtgcg ctatgcttgt
       61 cctgcaagaa atgggtatat ctgcattcgg tcttaactgt acatcagccg atttatgtcc
      121 cgatataata gaagagatcg ctccgtatgc aaagataccg cttatcgtaa aacccagcgc
      181 cgtgtttgaa gccaacggag aaaaacaaag catatcaccc gatgaatttg cgtttgcggt
      241 aaaaaaatca gtgatcaacg gtgcagggat agtcggcggt tgctgcggca caggaaaaga
      301 acatattgcc gctctgcgtg aaatgcttgc aaatatcgac aactcagaaa taaagcctgt
      361 cgaaaaacag gacacttctc ttgcgcttgc aactgaaaat cagatgttct tcttagatcc
      421 cgaaacaacg gagttttcac ccgcagtcga gtgcggtcct tatatggaag acgacattgc
      481 gcagatgtgc aacgaaagct acgatgtact tacggtaagc ataaactcgc ccgacgatgc
      541 tattgacttc ggcagaaata tgcatatggc aacactgcct gtagcattct tatctgacga
      601 tgaaatatcg cttaaaatgg cgcttatgct atatcagggc agggcgataa tagacaagaa
      661 atcacttata gagcccgaaa aactcgaggc tatggcagaa aaatacggtg ccgtgctgta
      721 ttaagcccta aacaatattt actttacaac acggaaggaa tttttgaaat gtataaatca
      781 gaaatgcaat actaccgcaa agcgttcttt atggcaatga taatgggtgt tatactattc
      841 ttgccttttg ttctcataga cggcggatat ttcatctatt acggggatta taacgctcag
      901 cagatacctt tctacacact ttgcaaccaa gctataaaaa gcggcagctt tatgtggaac
      961 tggcaaacag acctcggtgc gaactttatc ggttcgtact cattctacac actcggcagc
     1021 ccgttcttct ggctcagtat gccgttcccc gcagaatttg caaagtacct tatggcgccg
     1081 ctcatggtgt tgaagctgtc atgctgttcg ctgtttgctt ttgcttatat aaggcgtttt
     1141 gtaaaaaagc cgaagtccgc gcttatcggc ggtcttttat atgcattttc cggtttttcg
     1201 atgtacaatg tattcttcaa tcacttccac gaagcgatgg tcgtgttccc tcttatgctg
     1261 attgcgctcg aagaagccgt tgtaaacaag agaagagtgt tctttgcact tactgttgcg
     1321 ctcaacgcat ttgtaaacta tttctttttc atcggagaat gtatatttct tatcatctac
     1381 ttcctttgcc gtcttaccga cccgaaattc aaggtaacat taaagacatt ctttgcgctg
     1441 gcgtttgagt ctgttatcgg cgttgcgctg gcgggagcga tgttcatacc cgcgataata
     1501 acctgtatag ataccccgag atcgggcaat accttaaacg gctggaattt ggtgttccat
     1561 aatccgtcac agcgatatgg aataatattg cagagtcttt tcttccctgc cgatatccct
     1621 gcaagacaga atttcttccc ggattcgtca gcaagatggg cgagtgttgc cgcatatctg
     1681 ccactgttct caatgtcggg tgtaatttca tttataaaat tcaagaaaaa acactgggca
     1741 aagtggctta tgccgatatg cctgttgttc gcacttatac ccgtgctgaa ctcaagcttt
     1801 gtgcttttca ataataattt ctacacacga tggttctata tgcctatact cgttgcttgc
     1861 tttatgacct cctatgcact ggaaaactca gagatagata tgaagtacgg acttaagtgg
     1921 tgcggatttg ccgtagtgct tatatcactg gtgggtgtgc ttccgtcaga ggtatcaaaa
     1981 gatatagtag atataggaac cggcgatact actaccacaa aagtttccga gcttttcagt
     2041 ctgccgggcg aaaagctgcc gttctggata tcggtaatac ttgcgataac ggcaatagtc
     2101 gtatgcttta tactggttcg taaaaaggat aaagcaaagc ctaacaggtt tttgcataaa
     2161 gcgtactgct ttacaatagt tgcctgcctg tgcttctcat actacacgct tatctacgga
     2221 agagcgatag gtcctaaggt aggagattac aataacattg taaatgctac aatagagctt
     2281 gacgatgaca gcttctacag agtcgaaaca tacggcgtta caaacaatgc caatatgctc
     2341 tggggaatgt acggtttccg cagcttccac agcattctgc ccggctcttc gtttgaatac
     2401 tatga
//
LOCUS       DAWAOG010000317         2391 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5740_length_2391_cov_2.119435, whole genome shotgun sequence.
ACCESSION   DAWAOG010000317 DAWAOG010000000
VERSION     DAWAOG010000317.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5740_length_2391_cov_2.119435"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    322..331
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    854..863
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1691..1700
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          741 a          445 c          476 g          699 t
ORIGIN      
        1 tcggtatact cgtccatgag ctttttgtta ctttcatact gctcctgtac gatctccttg
       61 aagtccttat caagtccgcc gtccgacagc atctgttcag cctcttcata atcgttacgg
      121 gcagttttgt acattctgaa cttatcaatt atcggagtca ggctcttaag ctccttcatc
      181 agatttttat actgctcctg atcgtttatc acgtttatat ccgtcagctt ctcgtttatt
      241 tcatcgaagc gcttttccat tatttcaagt ttttctgtca ttgtttttct ccgtttcagt
      301 tattaagttt gatttatgac annnnnnnnn ntttgaaatc agcaccgaca cgcagtacga
      361 gcatttcttt gctgccgcac ggatgttctt ttttcatttt cagcgtatcg ccgacctgta
      421 tatccatcag ttccgctcct ttcatcgaca tttaatatat tgtaccacat ttttatcgga
      481 tttgcaattg gcgggtaaat attttatatg atttatccat tctgaatttc gtttgacgat
      541 atgggaataa tcataaaaag aaacggagga aaacaaaatg aaagcagaaa ttaccgcaag
      601 atttacagat gccgataccg cgcaacgtgt cgcaatatat ataaacgcct tatacaatgc
      661 cacatcggat atgtacacga cacagatcgc cgacggatat tacagctaca gcggcatagt
      721 tcccttgaca gttccgggtg ctaattatct tactaatata ttctgtcccg tatttcctga
      781 aaatacaacg cttgatgaaa tagaaagccg ttgtggcata acagcaaaaa ttcgttgcaa
      841 taaaaatgac gcgnnnnnnn nnnaaatcac aagatatatc ataaagaacg gcggtcgcat
      901 aatttactga aaatgcttgt gatttactgc attttataca aaaaagtgct tgattttgag
      961 tttgaaaaat gctataattt aaataacata tcagacaggt aaaatgattg atagggaggg
     1021 tgaccctgtg aaatctatac ggaataaatt tgtcctgctg atgataggct gtgtgttgat
     1081 atgctcgttg gctatcggca ttataagcgt tttaggcgtt gacagtatat caaaagaaga
     1141 tgcggaaact ataatgcaac tgcaggcatc taccagtaca gaaagtattg atcggctttt
     1201 ctctacggtt gagcttgcca tgagtgcctg ttatgattat gcgttcagcc gttttcacag
     1261 tatatccgaa tttcagaatg atcccgatag actgatagac tataatgatt ctgtaggtca
     1321 gcttataaag aacgttatgt ctaataccga ggcttctatc tgcggatata tccgatataa
     1381 tccggacttg aacctttctt ctgacggtgt attctgggta aaggacagca atagcaacaa
     1441 tatcaaagaa catgagctta ccgacataga aaagtacaaa aaagaagatt ccgaacacgt
     1501 tgcatggtat tacgaaccgc ttaaagcagg aaagcctgtc tggatagatc catacgttaa
     1561 caagaacctt gacaatgttt atatgatatc atatattata ccgttttatg atgaaagcgg
     1621 aacagcggtc ggagtcatcg gaatggatat tgatatgcgc cgcatcatct caatgataaa
     1681 cgagatagag nnnnnnnnnn agatagagct gtacgaaacg ggttacgctt ttctttgcga
     1741 cagtaagggc aacatagtat atcataaaca gtttccggac ggaatatctg tagatgaact
     1801 caaaaatgat aaaatggtga cggttgtcga taatcatttt gacgaagaag atgtcggcga
     1861 tgtaataact atactgaatt acgaaggcga aaggcgcaag ctgtgcgcaa aaaaattatc
     1921 aaacggtatg aatatcgtaa taaccgttcc tgagaaagaa ataaacgaaa agcgtaatat
     1981 tgtcgttatt caagacctga tcgcagttgt tatagcattg ttagttgctt cgttactgac
     2041 tttgcagttc accaacataa tcgtaaaacc gataaagcat cttacagagg tttcaaaaaa
     2101 aatctccgca ggcgatcttg atgtcgagat agagtgtaaa acaaaagatg aaatcggtgt
     2161 gcttgccgca cgttataacg ataccgtcaa aatgctcaaa aaatacatag ataagataaa
     2221 caaacaggct tataccgatg ccgcgaccgg tgtgggcaac aaggcagcat atcaggacgc
     2281 cgtacagcgt attgataaga tgagctgtca taataacggt gactatgctg tgtttgttat
     2341 ggatataaat taccttaaaa tgtacaacga caagtacggt cacgaatttg g
//
LOCUS       DAWAOG010000318         2390 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5745_length_2390_cov_2.425268, whole genome shotgun sequence.
ACCESSION   DAWAOG010000318 DAWAOG010000000
VERSION     DAWAOG010000318.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5745_length_2390_cov_2.425268"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1121..1130
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          773 a          473 c          574 g          560 t
ORIGIN      
        1 catatcaaga aacaggagag ggttattatt ggatgggaaa atacgaaacg tatgttaata
       61 aagtaacaga aaaatctgta ggtgaaatca gatattatga tttatctaag ggaaaatatt
      121 acatacagtt agatttatat gccagtggta gttcagagat acccgcaagc gaaacgataa
      181 aaatatctca tgagcattcg tattacggta cagtaacaag tgaatctacc tgtaccgaga
      241 aaggctcaat aaaatacacc tgcgattgcg gtgattctta tacggaagaa ataccgctta
      301 aatcccataa cgaagtaaca gatcacggaa aagcggcgac ctgtaccgaa acaggtctta
      361 ccgacggttc gcattgttcc gtctgccaaa aagtacttgt tgcgcagtcc gaaataccta
      421 aaacaaatca cgaatacaac gaatcatata aggtggtaaa agacgctacc tgtaccgaga
      481 agggcacaat aagatattac tgtgtctgcg gaaaagcaaa agatgaagaa ataccgttga
      541 ccgagcatga agcggtcata gatgaggctg ttgccgcaac ctgttcccaa agccgatcat
      601 actcctgttg cggataaagc gatcgctccc acttgtatta aatccggtaa aacagaaggc
      661 agccactgct ctgtatgcaa aaaagtactt aaagagcagg aaacggtagc agcaaccggc
      721 aagcatactc ttaaacatat tgcagagaaa aaagcaacag aaagttcttc tgccgtgcag
      781 gagcattgga aatgttctgt ttgcggacaa ttattcgccg atgaaaaagg cttgacagct
      841 ttaagccgga caccggtcaa agacgaaagc ccgcttatga tgataatagg aatatccgta
      901 ggcgcattgt tgctgatagg cggaactgtt acggcgatta taatatcgaa aaagagatcc
      961 ggtaaaaata cagatatcac aggcacagaa taatatgaaa acactgcctt gacaggacgt
     1021 tcacggcggt gttttcatat catttccact ccgcccgaat gcagtcgccc atttcgtgac
     1081 atactgatat aaagtcagcc tttatcgcaa taataaaatc nnnnnnnnnn tccgaaatct
     1141 agcggattga aaatcagaaa tcactcgtaa tgaaaacaca aaatctctcg ggttgaaaag
     1201 ccgaaatcta tcgtaatgat attgcaaaat ctatcgtaat gtgttatatt atttatacag
     1261 cttacgaaag gagcggaata tgcgtaacga taaatacaga cccagactga tagataagaa
     1321 aatagacgaa taccttaagg cgttcggagc ggtgtgcgtt gagggtgcta agtggtgcgg
     1381 caagacgtgg acatcggcgt ttcacgctga aagtcagata ttgataggca accccgaaaa
     1441 taactttcag aacagaaagc ttgccgaaat gtcgcccgaa ctcatactcg gcggtgaaac
     1501 tccccgactt atagacgaat ggcaggaagt accgccgata tgggacgctg tgcgttatga
     1561 ggttgataaa cgtgccgaaa agggacaatt tatattaaca ggctcggcaa cgcctaatca
     1621 taagggtatt atgcacagcg gtgcgggcag gatagcaaaa ataagaatgc gcccgatgtc
     1681 actgtatgaa tcgggcgatt cgtcgggtaa aatatctctt aaaagtatag cggacggcac
     1741 aatgcaggct gaaatgaccg gagaagtgga gcttatggag cttttccgcc ttatagtcag
     1801 aggcggctgg ccgggtaata taagcattcc gctgaaacaa gcaatgctaa ttcccgatga
     1861 atatctgaat gcggttatag atgatgatgc gttccgcata gacggcgtga agcggaatac
     1921 tcagaaaatg cgacttttgc ttaaatcgct tgcgagaaat gaaagcacga ccgcaacgaa
     1981 taaaaagctg aaaaacgata tatctcagac ggataacgag gatatcagcg ttgacacggt
     2041 agccgaatat cttgatatat tcgagcggct tttcattacc gataatcagt tgccgttttc
     2101 gtcaaatata cgctcatcgg ttcgggtaaa acaggcggta aagcggcatt ttgtggatcc
     2161 gtcgcttgcg tgtgcattgc ttggcgctac tcccgaaaga ttgcttggcg atcttgaatg
     2221 cgccggcttt atgtttgagg cgctgtgtga gcgtgacctg aggatatatg ccgatgtaaa
     2281 cgatggtgtg ctgtatcact atcaggacta ttcgggcaag gaaattgatg ccgttataga
     2341 atatccggac ggtgagtggc tcgcaataga aatcaagctc ggcgcaaatc
//
LOCUS       DAWAOG010000319         2386 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5757_length_2386_cov_2.555985, whole genome shotgun sequence.
ACCESSION   DAWAOG010000319 DAWAOG010000000
VERSION     DAWAOG010000319.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5757_length_2386_cov_2.555985"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          657 a          580 c          399 g          750 t
ORIGIN      
        1 caagtcaata tttatgacaa aactgtaact attttgttac cggtttgtaa cctttagaat
       61 cattccgaaa gtgtgtatgt atcaagaata aagcttccgc ttgcaacggt aacatcaacc
      121 cttgtatccg cattgccgta agaaacatta ccgcctcctg taagcttttc gtttcttccg
      181 tcaagaccga tttttaccga acccgaagca acgctgtatg ctacctgtgc ccctgtgtcc
      241 tttggtacgg agatatgcgc ctttccgctt gctacttttg catctatgcc tgtaatcttt
      301 tctgcacagc ctacagtaag agaacccgag gtaacatttg tactaagctc gccacctgca
      361 tttgatgaga ttatcttgcc cgaagtcaga tcgtaataac tcttttcact gacaaatccg
      421 tcaagcacga tcatcccgct gagagctgat acactaacat ttttgtactc atgtgtcctt
      481 gacgatattt ctgcttttcc ggacgataca ttgatatcga aattttcagc cgcaacgctc
      541 aaaataatct taccgctgta caacttgatc tttgtacttt tttcggtatt cggcatatct
      601 cccgacaacg atcccgaagc taccgtgata tctgcatttt caagttttct gttatctctg
      661 tagctttcgg gcagtgatat ttctatcttt gtcggaacgc tcgcaagcgt aaagagtgag
      721 aagaacgaaa atcctccctt ttccttgaca ttaagtacac cgtcagtgat attacattct
      781 atttttttat ttttatccaa tgtagtatag ttcaccttta cttcgtttgt gctgttatat
      841 gaaatatttg cgtttgcact gagtacgctt atatcaagcc cggaaaatgt atcggtaaat
      901 gtaaactcct gcttttccga tttgctgtca ataataccat tcatatactt ctctccttcc
      961 agtactgtcg gcaatgccgt aaatacgcag cctaaaattc ctaccgcaag caaagcggct
     1021 gatattataa gtatgatctt cttcatcaga aacctgccct tcttcctgta aatgccttta
     1081 catgaagtct tgctatcgct ttaatcatgc cggcaaaacc tctcacaaca ttcggtacaa
     1141 atacagccat aatgccaccc agccccagta ttgcaagccc cgcaaacaca ttaaaaagtc
     1201 cgccgaataa aacggttgta atactgtctg cgacaaacgg cataaatacc gcagctgtat
     1261 tcgcaatccc tgatacaata aatgcaaatg ctactgcaat ataagttaca acaagtgccg
     1321 ctactgtcgg tatcgcccaa tcaagcacaa ataagtcaag caatacaact ccgactatca
     1381 ttccgccgct tattttgctt gtatcaggct ctgtcggatt tgttctgtac gcggtttttt
     1441 gcatattgtt tccgctatta tttgtgttgt cggcggcttc tcccgcatat tcctttgcta
     1501 tttccgcagg gtcgccgagc tttgcacaaa tagcgctttc gctttcgcca ttctcagcgc
     1561 cttcggaaaa atgctgttca aaatcctctg tcatttcatc aaacggcaat gaataccttg
     1621 ccgcttcttt ttgcaaagct gccatgaact ccgcctttgt attataagtc atattaaaat
     1681 ccctttcatc gttcaatgcc gagtatctta tttactgatt tgtagaaatc tgtccattcg
     1741 ctgatttgta ctgctaactt ttttctgccg acatcagtca gtgaataata ctttcttgat
     1801 ggtccttcat tcgattcaac aagatatgaa gtaatgcacc cctcgtcctt taaccgcttc
     1861 ataagcggat aaatcgtccc ctctgtgata tggatactct gcgaaattgc atttacaagc
     1921 tcataaccgt agcaatcacg atctgacagc agagccagca cacatagttc cagcgttcct
     1981 cttttaagct gtggattcat acaccgctcc tttctgtttc gttgtactaa gtataacaca
     2041 atgtaccttg cattgcaagg tagctagcga taaaatatag ccgaatatct cggtaacatt
     2101 ttgtgaaata tgaccaaacg catcataaca ctgtctgaaa ctgttgtatc cggcttattt
     2161 atgcataaaa aggagctgtg acacaattct tcagcttatc aaattacatt aaaattacaa
     2221 tgcaatatat tgtaaaaaag tctgagcaat aaatatacag ccaacaccgc taaaaacagt
     2281 atccgtgcca acaagttgtt aataatttcg tgagtgtatc aatgcaactg tactgtcacc
     2341 gaaatttcca aaaaacgcac tatcccaaga tatctgtaca gtcccc
//
LOCUS       DAWAOG010000320         2384 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5761_length_2384_cov_2.603263, whole genome shotgun sequence.
ACCESSION   DAWAOG010000320 DAWAOG010000000
VERSION     DAWAOG010000320.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5761_length_2384_cov_2.603263"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    233..242
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          709 a          422 c          649 g          594 t
ORIGIN      
        1 gaaaccgtgt tctttctgtc tctggagctt cttaggctgg tatgttcttt tcatttcttt
       61 cttcctcctg ttaatacttg acaccgatat gtgtctgtct tgcgcctatg caatttaata
      121 ttatacctta atcgtccgct gatgtcaaga catattttta aatttgtccg ttttacttgc
      181 ggcaacgttg acgattttcg taaaacaggc aagaggcaga caaaacatcg gcnnnnnnnn
      241 nntgagattg acatttgaaa cgatgtatag tataatattt ttgaagtcaa taaagatcat
      301 gtggaaagga aacaacaatg aaaaagataa cagcaatact attatgtgcg gtaatgtgcg
      361 tgtttattgc gggttgcagc agcgaacccg tacaggaaag cagtgaacaa cagatatctt
      421 cggatactgt aagttccgca ccggaagttc ccgcaccgga cggtttcaga gttgaaggca
      481 caaagcttat cgacggctat ggcgaagagt ttgtgatgcg cggtgtgaac cacgcttatg
      541 catggttcaa gagcgatacg gataacgcga tagagggcat taaatatgta ggttcaaata
      601 cggttcgtct tgtactgtcc gacggcgatc agtgggacaa ggtgacaaga gcggagctga
      661 agagccttat aaggaaatgc aagaaaaatc aactcgtggc agtgcttgaa atacatgacg
      721 gtgccggtaa ggacgatgtt tctttcctta ataatgcagt tgattactgg ataggcatga
      781 aggagctgct gaacgaaaat aaggcttatg ttctcgtcaa tatagccaac gagtggtacg
      841 gcaaatatga taagcttgaa gtatggcgtg atgcctatat cgatgcggta aagaagctga
      901 gagatgcagg cattgagaat actctcgtag ttgacagtgc aggctgggga cagtgtgcgg
      961 acagtgttct gaattatggt gaggaaatac tgaaagccga taaggacgca aacacaatgt
     1021 ttgcggtgca tatgtacggt acagctggaa agaacgaaaa gacaatcaaa aacaccattg
     1081 atacagctca aaagaagaat ctttgtctgc tgatagggga gttcggctgg aaccatacag
     1141 acggtaaggt cgactttgaa acgataatgc agtactcgac agagaagaat gtcggatatc
     1201 ttgcgtggtc gtggaagggt aacggtgatg atgtaagcta tcttgacgtt tcgcgcgact
     1261 gggcgggcgt tgacctgaca tccgactggg gcaagccgct ggttgagggc gagtatggta
     1321 taaagaagac atcaaaggta tgttcggttt atacaaagta tgcgaccgat gatgaggaca
     1381 gcgcagacga ggcggttgac cctgcagagt aatcaataaa atatggtata taccaccggt
     1441 atactttgct gtgtaccggt ggttgcattt tatgtgtttt ttgtagtata atcgatattg
     1501 gtaaaacgga tataaaagca gttgacaaag gctcgggaaa tgcaattgtt ttttatcaaa
     1561 tgaaaatata atcattaggg agtttatata tgaatagtat aaaggtagca ctgttgcagc
     1621 ttgatccgga aggaaattta cagggtaata tcgaaaaggg gatagtggca gttgaaaaag
     1681 ctgcaaggat gggagcagat attgcgcttt tcccggaaat gtggagctgc ggatatgatt
     1741 ttccgcagga caaaaaagca ctgtgtgagc ttgcggtaag caagaacagc agttacacag
     1801 acgcattcag aaaggcggct gttgacaacg atatagcagt ggcggcgaca tatcttgcgg
     1861 aaaacggtga caaagcgcct ttcaatgcga tgacactgtt tgacaggtac ggagcggaaa
     1921 agttcactta ttataaggta cacacctgcg acttcggaga tgaaagagtg ttgtcacacg
     1981 gagatgagtt tttcactgct gagcttgaca caaagagcgg aaaagtaacg gtcggggcga
     2041 tgatatgcta tgacagggaa tttcccgaaa gcgcaagaat acttatgctg aaaggtgcgg
     2101 agctgatact tgtgccgaat gcctgtccta tggaaataaa ccgtatatca cagctcaggg
     2161 cgagggcata tgagaatatg acgggcattg ctacggtaaa ttatccttat ggcaaaccgg
     2221 actgcaacgg ccattctacc gcctttgacg gcatagcata cagagttgat ggcagcggcg
     2281 gaagagatac tctggtgata gaggcgggcg agcgcgaggg catatacatt gccgatttcc
     2341 ctatagatga tatgcgggag taccgccgca gagaagtaca cggc
//
LOCUS       DAWAOG010000321         2379 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5782_length_2379_cov_2.267212, whole genome shotgun sequence.
ACCESSION   DAWAOG010000321 DAWAOG010000000
VERSION     DAWAOG010000321.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5782_length_2379_cov_2.267212"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          634 a          551 c          537 g          657 t
ORIGIN      
        1 gtatgagggt gaacagcaga aagctcatct taaacgcgca atcacatggt atacgaatgt
       61 attttggtgt gcgcttataa atcttgctga cctagagcac aagaacgaaa cgatgtctga
      121 cgcggaaaga ataaaaattc tgaaaaaggc gctctcaatg tttgagcttg tgtttgagaa
      181 cggtgattat cttaagtaca gctgtacggt gtcggctaca catcggtata tagcggatct
      241 ggctgtgtcg aagggtgatt acgagcttgc gttatcaagc tttgaaaaag cggctgaatt
      301 tgcgataatg tcagacactc tccccgataa ggcacatcac acatctcttc ttgtgaatga
      361 ccttgaatac agcccgatca atctggtcaa gaattacgat ttcactgact gcagggaatt
      421 atacgataaa atgcagtcgg acagatacga tgtaatccgt gacgataagc ggtttatagc
      481 cgcgcttgag aaaatcggca ggtattgtta attcggagtg cggcaactat ctataaaatc
      541 cgcaaaggcg cttgaggtgc ttgcaccgag gacggtggat ataaaagctt cacattccca
      601 cggttgataa taacctacgc ataagcaaag aaccccgcat ttgcggggtt caaactgttt
      661 ataaagtgtt cagcttactt accgagtgca atcttaccca tagcgtcagc ctgcataata
      721 gcgtcataca gctcaacggg ctgtacatcg cctgcgaggt tgtggatagt ttcgccgggg
      781 atacaagcgt tctcggagat cgtctttacc tgctcaggtg tagtaacgcc gatctcttca
      841 agtgttacgg gaagtcctac ctcaaggcag aagtcctgta cttccttaaa ttcttccttg
      901 ggtgcgccct caagtacaag ctgtacaagt gtgccgagcg caacacagca gccgtgatct
      961 gcgtgcttta ttccgagaga agttacgccg ttgtagaacg aatgagcggc ggcacagttc
     1021 acgttatctg caccgacacc ggagaggtaa acattagcct caacgatatt ctcaagcgcg
     1081 ggagtaactg cgtgtacctt acaagcctca atagcctgct tgccatattc gcggagcgtg
     1141 tcgtagcaaa gacgtgcgag tgcctgtccc gctcttgtga tacctgtacc ctcaaggata
     1201 gcggactcag tacggataga agcacgaccc tcaaaatatg tgccgagcgc atcgcccata
     1261 cctgctataa ggaatttaac cggcgcattg gcgataacgg tcgtatcgac cattacggca
     1321 tcgggatttg tggggtagaa cagatacttg tcaaagctgt ggtcgtcatt gtagattacc
     1381 gaaagacctg tgcagggagc atctgtagcc gcaactgtag gcacgataac aatgtgtctg
     1441 ccgctgtaat atgcggtagc cttagctgta tcaacggcac ttccgccgcc tacagctaca
     1501 actgtatcaa tattatcctg ctcgacaata gccttcatct tgtttatttc gccgttgctg
     1561 gatattccgc cgaaaatctc gtaacgtctg tagtcgtcaa gaccgtcaaa gctcttttct
     1621 atcttgtcat ggcaggcttt gtagccgctg tttgagcata caaacagcca acgcttgccc
     1681 atataaccca tttcttcatg gaatttaaga agagcatctc tgccctgaac atatttgagc
     1741 ggttcacgca tcgctctgag tcttcccatc ataattgaag cacctccgta gatttataga
     1801 aattagcgga tattatatcc gtttttacga cttatttata tcacatttta ggagcaatgt
     1861 caatatttat gggcattccg atttttgata ttgatatgct gaattttcat tactgagaat
     1921 tttaagccaa atcaaataag cagttacact gtttatcgcc ggatcataag atgataaaca
     1981 atgtaactgc ttattatttg cggaagaaat tttaatgctt tttcttttgc gcgttaattt
     2041 ccaaagtaag ctccgctttc ttttccgctg tttaacttac atcggaaata aacgtataaa
     2101 gtcggcgaga gtcagacttc tgcgtactgc tttctgcctg tttcaaatat atttctacac
     2161 tttcacagcc gaggtcttca aatgcaataa cctcgcactc tgtgatacat tggcaggcaa
     2221 gcgcacctgc gcctcctatt gcacagaaat atactgcgcc gttgcgtttt atggcatcga
     2281 ccactgccgg tttacgttca cccttgccta tcattgcaga aagccccata tcaaggagca
     2341 gaggagcata cggatccatt ctgcccgatg ttgtaggac
//
LOCUS       DAWAOG010000322         2375 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5800_length_2375_cov_2.162500, whole genome shotgun sequence.
ACCESSION   DAWAOG010000322 DAWAOG010000000
VERSION     DAWAOG010000322.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5800_length_2375_cov_2.162500"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1003..1012
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          719 a          552 c          411 g          683 t
ORIGIN      
        1 gcatccttct ttgtaaggtc agccttttct gcaaccattg taactaattc tgcttttgtc
       61 atttgagtaa tcctccaaaa aattttaaaa ttatttaagc tgaaaagctt attaacgtaa
      121 tacggtcaac cgactaattg ctttacggtt aatcagaatt taaaaacagc gcttttatct
      181 gctcatctga cagattgtcg agcgttttgc cttgcgactt cgtattactc tcaagctttc
      241 taaatcgcat tataaactta tttacagcaa aagtcaaggc tttttcgcaa tctttttcga
      301 taaaacgtga taaattacag caagagagta aaagttctcc aaatttttct tcggtaaatt
      361 cttcgttttt gtcatttata gctaatttaa gttcatcaaa tttaccttga acaagatcaa
      421 gtgcttcttt agtatcgcac atttccacac cggcgtttga tgcacgttta aatactttct
      481 gtccgcgcat aagagcaggt aaaaccatag gcacactgtc aagtgtatcg gctacagtat
      541 cctgatgctt agattttttc ttcaacttat cccaattttt aagaacatcg tcactgttat
      601 caactttcac attgccgaaa acgtgaggat gacggtaaat catcttcttt gtgacatcat
      661 cacatatatc attaaattca aatctgccgc gttccgtttc tatctgacaa tgaaaaaccg
      721 cctgaaacag cagatcgccg agctcttctt tcagcatagc cgcatcgtcg gcatcaattg
      781 cttccataac ctcgtacgct tcttcaagaa cattcatacg gatcgactta tggtcctgtt
      841 ccttgtccca cgggcaaccg ttctcgcttc tgagaatacg ggtaacttca attagatcgt
      901 ttatgtcgta cttatcttta aacttaaagt ccaaaatata cacttcctaa ataaaaatag
      961 cacaggtgcg tatataataa tacactaaaa tctgtcatat atnnnnnnnn nnctattttc
     1021 gcattttttt cgtttttggc gatttagcaa agattttata ttgatttcgt tgaataatat
     1081 cagtaaaatt atataaacaa aaccgcctgc tccgattgac aataacaatc ttataaaagc
     1141 aaccgaccgt tcaaacagta tatttgcgct cagataagct ataaccgcac atactgtacc
     1201 tgcgataagc ggaagtgaca tttttctcag aatatgaaaa tctataccgg tcacagcttc
     1261 cagagcaaaa tagcctccca gcgcaacaaa cgcatacatc acaactgttg atacggcagc
     1321 accgcttata tttatttcgg gtatcgaaat tgtaaacaca ttcattataa gcttaagtgc
     1381 gcatcccggc agcatcagct ttatcggcag gtcggaacga cctatgacct gaaaaacaga
     1441 aataaaggta gatgacaggg taagaaatac tccgccaagc cctaaaatga acagcggaag
     1501 aacacttacc tcgatctcct gttgccttcc tgaaaacaga aaatcaagta tatacggact
     1561 cagcgccgaa agaccgagat acaacggaaa tcctatgatg aaattcgatc gtagtactat
     1621 cgaaacctta cgttcaaaat ctcccttgtt tttcaatgtc cacgccccgg ctatcctagg
     1681 aagcgcactt cttccgaaca ttgccgtaaa tgacggtatc agcataaaca ttgtccacga
     1741 aagtcctgta tagctcccgt acataaagtt cgcaagcctg actgcaccgc cctgagaacc
     1801 gataatttcc gaaaaccgta ccgaaaagta ctccggagcg catgaaagcg cataattaag
     1861 gcatcgaggt atagtaataa gatctataaa cgacgaaaga tttattacaa cagccgccgc
     1921 tgatatcggc acacagttct ttaacagctc ccgcacaatt gcgaatgttc tgtcagtcga
     1981 ggtgtcgcct ttatgtatat cctttcggca cagcttcttt cttataatca ggcagacgag
     2041 cgcacaaagt tcacttgcgg ttacggcaag aacagcacct gctgcggcgt acggcattat
     2101 aatatctgtt gcctgtgtta tgttatcggc taacgtgccg aatatttcct tgccgctgtc
     2161 ataacacttt attccatagt ttaatataaa atacgatgtg ccgagcccca caatggcacg
     2221 gcagaccgat tccgcgacct gtgataccgc tgacggcatc atattacagc atccttcgta
     2281 atacccctta taaaccgacg agatgcagca aagacataca gatggagcta taattataat
     2341 gccccatacg ctttgcggcg actgagcgac ataat
//
LOCUS       DAWAOG010000323         2364 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5837_length_2364_cov_1.691641, whole genome shotgun sequence.
ACCESSION   DAWAOG010000323 DAWAOG010000000
VERSION     DAWAOG010000323.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5837_length_2364_cov_1.691641"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    221..230
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1109..1118
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          556 a          565 c          477 g          746 t
ORIGIN      
        1 ttctgaagtg ctctgtctgt aacgtcgatt attcttgcgt ccttctcctg atatctcttg
       61 agttcgtttg caacatcttc gtcagttaca gtcttgacag tcttgtttac ctttataccc
      121 ttatagtcct taacttctac ctcgggcttt gttgtgaatg tagccttgaa agaaacaccg
      181 ttctccttgc ttacatctgt tacttcgata ttgggcatat nnnnnnnnnn agcttgagct
      241 cgtcgataac ctcggcaacg ctcttctggt acataccgtt taccgcatct tcatagaaaa
      301 caccctcgcc gtactcagcc tctattatct tacggggtgc cttacctttt ctgaagccgg
      361 gaacgttgat gttctttttc ttctttaaat aagctgcctg tactgcctgc tcaaattctt
      421 cggctgttgc cttgaattct acctctaccg tatttgtagc ggtcttgttg tttgaaatga
      481 tctccatttg aaaaatcctc cgtttttgca aagccttgta tacggcgatg cgtattattg
      541 tattttattc ctgcacaagt acaggtgtaa atttaacata gtcagccgac accattctgt
      601 aggtaattcc gtccttcact ccctgaaacg ctttaggaaa acacggagcg ctgtgaacgt
      661 gaccatagaa gcaacgcttc acaggatacc ttgacattac atcaagaatg tagtcattgt
      721 gctcactgcc atatattggc gggtaatgta taaacacaac aggttcaaga ccatctccag
      781 tctgcccgct tcacgcgcaa gaacctttgc gtcctgcgcc tctccgtcat catttatcca
      841 gcctcttgtt ccacatatcg ctatgccgca ttcgctgaac gcgttgttgt gcattatcct
      901 gatattgtgg aaatcgtttg actgtaaaaa gctgttcatt ttgttaagtg tagtccacca
      961 gtaatcgtgg ttgccctttg agattatctt cctgccgtgt agttttttac tcacaaactc
     1021 aaaatcagga agtgcctcct ccagcgacat agcccagcaa agatcaccgg gtatcacaac
     1081 gcaatcatct tcaccgacta tactgttcnn nnnnnnnntg aagatcaccc attgtgtata
     1141 ctttcaaata aacgctcctt aattacttgt ttataaattc gagaactgcc tttgacattt
     1201 cttcgggctt tattgaacct gtaaatctta caggcttgtt ctttgtatcg gaaagcgcct
     1261 tggggcaagt tgtcttagtc agcttttcaa gcttgccgat aagctcgaac tcgtcaacac
     1321 cctcagtttc gccgcccagc gcatcgtata cagcaaagcc gaacttgtag gggttagcag
     1381 tagaagcaat aagagtcttt gtgttgtcgc ctgtattctt cttgtaatcc tcatatacct
     1441 tgtaagcaac ggcagtgtga gtatccataa ggtagctgta ttcgccgaat acttccttga
     1501 tagcaccctt tgtttcctca tcgttgcagc agcctgcata gaagttttcc ttgatatcag
     1561 cctttacgct ttcatttacc tcgtatctgc cgcttgtctt aagagctgtg aaccattcgt
     1621 tgatcagctt gtcgtcttca cctgtgaggt gatacagcag tctttcgagg ttgcttgaaa
     1681 taaggatatc catagatgga gaaacagttg tgtagaactt tctgtttctg tcatagatac
     1741 ctgtgtttat aaagtcggta agcacgttgt tggcgtttga cgcacatatc agcttgccta
     1801 tcgggatacc catctgctta gcgtaatatg cggcaagtat gttaccgaag ttacctgtag
     1861 gaactacaac gttgagcttc tcgccgtatt tcagttcgcc gtccttgacg agctgtacat
     1921 aagaagaaac atagtaaatt atctggggag caagtctgcc ccagttgatg gagtttgcgc
     1981 ttgagaatac cgtattagcc ttttcaagct cggcaattac agccttatca gtgaatatct
     2041 gcttaacgcc gctctggcag tcgtcaaagt tgccgacaac agcgcaaacg tttacgttgc
     2101 ttccctcctg agttgtcatc tgacgcttct gcatattgct tacgccgtcc tcagggtaga
     2161 ataccattat tgaagtaccg tcaacgtcct tgaagccttc aagagcggct ttgcctgtat
     2221 cgccgcttgt agcaacgagg atagatatct tcttaccgtc aattgtcttc tttgcggaag
     2281 ttgtgagcag ataaggaaga atttgaagcg ccatatcctt gaaagcacag gtaggtccat
     2341 gccacagctc aagaatgtat gtac
//
LOCUS       DAWAOG010000324         2353 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5876_length_2353_cov_2.170148, whole genome shotgun sequence.
ACCESSION   DAWAOG010000324 DAWAOG010000000
VERSION     DAWAOG010000324.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5876_length_2353_cov_2.170148"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1665..1674
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          560 a          592 c          447 g          744 t
ORIGIN      
        1 cccccgcaca cacggcacat ctcgaatgca agcacgggtc ttatacgctt accgcccgct
       61 tccagactgt atgccgccgc atcataaagc gccttgtatt tcgcgccgct gtcgcagaac
      121 gaattgcaga cagctttcag ctgttcctca aaatattcgc ggtatgcctt gaacatagcc
      181 tcaaattctt cttttgtcga aggagtgccg atcagtttta tctgcatatt acgcctcttc
      241 cagcttctgc acagtaagct tggcttcgtc aatatacttg cgacagaacg cgataagctc
      301 caccgcttct ttatagcatt tcatcgagtt gtcgagtgtg atatcaggct gttccatctg
      361 caccgatatc tcgtcgagtc ttttcatcgc tgtttcaaag ctcatttaat atgcacctca
      421 ttgctttttt ctgtttttgt tatcatagct cttgcagtgc cgtccgacat cagtatttct
      481 acagtgccgc cgacagatat atcatcaacg ctgtttacag cttttccgtt taaggtaacg
      541 gcactatatc ctcgtctgag tacattcaca ggactaagcg cttctatctt atctgtgtag
      601 ccttcaataa gcacctcatt gcggtgaaca gttgcgctta ttgctgtttt gatcttgccg
      661 ccgagcaatt caagctctct ttgcgaccgt tcaagacgat ttatcgggct gtatgccgat
      721 atatgtcgct gatagccgtc tatcagctgt tcatacccct ctatctgaga gtgaactata
      781 tcgctgagct tgtcgtatgt cagctctata tgttcgctaa gatcctcaca ggtgatcgat
      841 gtggcaattt cagccgctgc agacggtgtc ggcgcacgca gatccgcaac aaaatccgat
      901 attgtaaaat ccgtttcatg tcccactgcc gatattgtcg gtatttcact gctgtatagc
      961 gctttcgcgt atgctatgtc attaaaacac gacaaatcct cagcagaacc gccgcctcta
     1021 cctattatga taaggtcagc gcctgtcgtt tgtgccgccg taatgccgtt tataagtgtt
     1081 gcaggtgcat atgcaccctg cacggtcaca gggataaaca ctacttcaag tatcggtctg
     1141 cgccttgcga tgatattgag catatcctga agagccgcac ccttttctgc cgttatcaga
     1201 cagaccttat gcggttgttt cggtatcgga cgttttctgt caaaatatcc gcctgcttca
     1261 agctccctct ttgccttttc aaaggcaagc gcaagctctc cctgaccctt ttcgatgatc
     1321 tcatttacat atatctgatt tgcaccgtca cgttcatata catttacacc gccgtgtact
     1381 attacgctct gaccgctgta cggcataaaa gttagacctg cggcttttcc tgcaaacata
     1441 acacatttta tggatgaagt ttcatcctta agtgtaaaat acatatgacc tgacgcttta
     1501 tgcagagtaa agtttgatat ttctcccgca acataaacat cggacagtgt tttatcacct
     1561 tttacaagca tcgaaagtcg tcttgtgacc tgtgaaacgg ttacggtaaa aaattcagac
     1621 atcaggctgt tccttgttgt taagactatc cttgtagtaa gaatnnnnnn nnnntgagca
     1681 ctccgtttat aaagcgcaca tctgccttgt ccgtatattc cttggcaaat tctatcgctt
     1741 cgtttatcgc aaccttatcc ggaactgacg gacagtactt catctcataa acagctatct
     1801 tcatgatagc aagatttatg taggaaatcc tgtcgagttt tcttgacggc gaatagctct
     1861 ctattattgc attaagctca tcttcgtgtt cttctacccc gtttgcgagc tttaccgact
     1921 gcgagttata ttccatctca aatgattcct tgcagtcctc aagcagttca tcaagagttt
     1981 ccggattaag ttttctctgg aaaaggatca gcaatgccgc ttctctcatt tcgtgacgtg
     2041 taagttttct ttctgccatt taatttattt tcctttattt attcagctga cttagaaagc
     2101 ataatatcag caatttttac attaaccttt gatacggaaa ttccggccat gttctgtata
     2161 gcggacttta cgctctcctg aaccgactgt gctacatttt ccgctttaaa tccttgtaat
     2221 acgacaatgt ttaccgttac ttcaacggaa tcgttcttaa gcacagcctt aacaggagaa
     2281 gacggcataa gttttccggt gatctgcgcc atattcttat cgctaacaac aagatgttcc
     2341 ccctcagatg caa
//
LOCUS       DAWAOG010000325         2351 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5881_length_2351_cov_2.541376, whole genome shotgun sequence.
ACCESSION   DAWAOG010000325 DAWAOG010000000
VERSION     DAWAOG010000325.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5881_length_2351_cov_2.541376"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          731 a          506 c          599 g          515 t
ORIGIN      
        1 tttaagccaa aaggaaagga tgatcttgat atgctgaaca ctaataacct gacacagaaa
       61 tcaatggaag ctataagcac agctcagagt attgctgttt cgtatcagaa tatgaatata
      121 gaacaacagc acgttttact tgcactgttg caggctgagg acggacttat cccacagctc
      181 atcaagaaga tgggagcaga cgcttcacag ctgacaaagt taaccgagca gagtgccgca
      241 ggtataccta aagtaaccgg cagcggcaga gatcctgaaa aggtgtatgt atcccccgat
      301 gtggataagg cattcactgc cgcagaagaa aaggcaaagc agatgaagga cgagtatatc
      361 tcggtagaac atctgttcat cggacttctc gaaaagccga actcgcagtt aaaggatatt
      421 ttctctaaat gcggaattac cgaaaaagca ttcttacagg cacttgaaca ggtaagaggc
      481 agtgtgcgtg ttaccggaca gaaccccgaa gaaacctatg acgttctcaa aaagtacggt
      541 caggatctga cggagctggc acgtcagaac aagcttgacc ccgttatcgg cagagatacg
      601 gaaatcagaa acgttatccg tattctttcc cgtaagacaa agaacaaccc tgtgctgata
      661 ggcgaacccg gtgtaggtaa aacggctatt gcagagggtc tggcactgcg tattgtgcgt
      721 ggtgatgttc ccgagaactt aaaggaacgt cagatattct cactggatat gggcgcactt
      781 gttgcaggtg caaaatacag aggcgaattt gaggaaagac ttaaagcggt attgcagacc
      841 ataaagaagt cagagggtca gataatactg ttcatagatg agcttcatac tatagtcggt
      901 gcaggcaaga cagacggcgc gatggacgca ggcaacctgc tgaagcccct gcttgcaaga
      961 ggtgagctcc actgcatagg tgcaacaacg ctcaacgagt acaggcagta catcgaaaag
     1021 gatgccgctc ttgaaagacg tttccagccg gttatggtaa acgagcctac agtcgaggat
     1081 acaatatcga tactgcgtgg tctaaaggaa cgttacgagg tattccacgg cgttaaaatt
     1141 cacgactcgg cgcttattgc cgccgctact ttatcagaca gatatatctc tgatagattt
     1201 ttacccgata aggcgatcga ccttgtagat gaagcgtgtg cgcttatcaa gaccgaaatg
     1261 gaatctatgc cgtccgagct tgatgatatc cgcagaaaga taatgcagca tgagatagaa
     1321 gaagccgcac ttaaaaagga aaccgaccgt atctccgttg aacatcttgc cgaggtacag
     1381 caggagcttg ccgaaatgcg ttcaaagttc aacgagatga aggcaaagtg ggagaacgag
     1441 cgtaacgcta tttcaaaggt acaaaagctc cgtgaagaaa ttgaaagcac caacggccta
     1501 atcgagcagg ccgaaagaag ctatgatttg aacaaagccg ctgagcttaa atacggcaag
     1561 ctccctcagt tgcaaaaaga gctggaggaa gaagaaaagc tcgcagagca gtcaaaatct
     1621 gcgtctatgc tgcacgataa ggtaacagag gaagagatcg cgaaaattat ctgccgctgg
     1681 acaggtatcc ctgtcgcaaa gctgatggag ggcgagcgtg aaaaactgct ccatatggaa
     1741 gatatacttc ataagcgtgt aatgaaaagg taagcgaggc tatactgcgt tcaagggcag
     1801 gtatcgcaaa tcccgatcag cctataggtt cgttcctgtt cttaggtcct accggtgtag
     1861 gtaaaacaga gcttgcaaag gctcttgccg aggctctgtt tgacgatgag cacagcatgg
     1921 tgcgtatcga tatgtcggag tacatggaga aattcagcgt gagccgtctt atcggtgcgc
     1981 ctccaggata tgtcggatat gaagaaggcg gtcagctgac cgaagctgta agaagaaaac
     2041 cctactcggt tgtacttctc gatgaagtcg aaaaggctca ccccgatgta ttcaatatcc
     2101 tgttacagat tctcgatgac ggtagaatta ccgactctca gggcagaaca gtagacttca
     2161 agaatactat tatcattctg acaagtaacc tcggctcatc gtatatcctt gacggtatca
     2221 acgataaagg cgagatttcc gatgaggcta agaatgaggt caacaagctg cttaaaacac
     2281 agttcagacc ggaatttctc aaccgtcttg atgagatcgt attctacaag ccgctccgca
     2341 aggacgaaat a
//
LOCUS       DAWAOG010000326         2345 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5897_length_2345_cov_1.903057, whole genome shotgun sequence.
ACCESSION   DAWAOG010000326 DAWAOG010000000
VERSION     DAWAOG010000326.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5897_length_2345_cov_1.903057"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1913..1922
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          764 a          515 c          522 g          534 t
ORIGIN      
        1 aaatataaag ctttcgtata tgacagcggt gaactccaac ccatcgaaaa acccgatgcc
       61 atacgccttt cggatcttaa gaaatatgaa gcgcaaagaa agcagatcgt tgacaacaca
      121 agagcattca tagcaggtca ccccgcagac aacgctctgc tctacggcga cagaggtaca
      181 ggtaaatctt caaccgtcaa ggcattgctg aacgaatatg caaacctgcg tatagttcag
      241 gtggacaaga aagacgttgc ctgcctgccg acactgtata aaactctcag cgcaagcccg
      301 ctaaagttta ttattttcat agacgacctg tcattcagtg agaacgaaga gggcttcggt
      361 gtgctgaaaa aggttcttga aggttcagta tgcggcagac cgtcaaatat cctgatatac
      421 gcaaccacaa accgccgtca tctcatcaag gaaaccgaaa gcagtcgtat gggcgataat
      481 gttcacagtg cagatgaaat tgacgagagt atgtcattgt ccgacagatt tggtctgttt
      541 gtaacattcc ttgcgccaaa caaggaaaca taccttgaaa ttgtacgcag tcttatcctc
      601 gacagaggaa ttataataga taaggacaag cttgacagat gcgcggaaag atttgcactt
      661 aagcgtaacg gcagatcacc gagaatagca aggcagtttg ctgacagtct gcagtcaaag
      721 cttgcactcg gcatcgaccc ggagaaaatg taacatgaaa agaaagttta taattccaac
      781 agctgctctg ctggcactta cgatcacatt ctgcggctgt gaaaatatta tgctgatccc
      841 cggaaaatca tcgggcgcaa ttgtcgaaga aagcagttca tcgcaaagca gtgaaccgct
      901 cagtgcattc ccggtaacac ttaacgatac ggagataacc aaagctccca aaaagatagt
      961 atcgcttacc ccggcataca ccgagatact ttttgaaatg ggatacggag aaaaaataac
     1021 cgcagtaagc gactactgcg actacccgga tagcgtaaaa gagctcccca aggcggcgag
     1081 cagtgcaaac ccggacatta cagcgatcaa aaagctgaag ccggatctag tcatcacagc
     1141 gacccctatc gtaaccaagg acaagatatc gcttgaagca cagggtatca aagtgctggt
     1201 tatcccctcg cccagaagca tagaagaatt tgaaaatgtg tataagttct tcggacttgc
     1261 tatgaacgga ctgtttgacg gtgaagcggc aggagaaaaa gcttttgcac cgataaaaaa
     1321 gcagcttgcc gcgataaaaa agacaaaaaa gaaattcata tatgttacag ccgctaatac
     1381 tccggcaggc ggagatacgt ttgaaagtgc tgttctgagc cttttcggca caaatattgc
     1441 agaaagcgcg agcggctata catataaggc ggcagatctt gccgaaaatc agcctgatct
     1501 tatattcgta agcgatatca taggcgaaga tacactgata tccgatgaaa actattcgga
     1561 gcttaaagcg gtaacggacg gtaagattac ggttcttgaa aacagatatt ttgaacgtcc
     1621 gagtagcagg ataaccgagc ttatcggcac tgcggcaaag gcattcggag ttgacctgaa
     1681 tgaaaaaacc gaaacggaaa acagttctgc ccccgcttca gaaagtgaaa attcctcgga
     1741 tacaaaaaac aacagtcaag acaatccaca ggcaacagaa taattcttaa aatagcactc
     1801 ttatcgggtg ctattttctt ttattcctta tcgattgccc attatcacaa aaaaatattg
     1861 actttttgcc aaaatcggat ataattaaat atgtatgttc aactaaaaat cgnnnnnnnn
     1921 nnatcgggta ctgctttttg cgtccctgat gataaacgga aggaatcata aatcatatga
     1981 aatggacagg acttaacgat cttagagaaa gctatctaaa atttttcgag agcaaaggac
     2041 atctgagaat ggcatcggct ccgctcgtgc cgcagggaga taactctgta cttctcatca
     2101 atgcaggtat gacacctctt aaaaagtatt ttcagggcat tgaagagcct cccagacacc
     2161 gtgtaacaac ttgtcagaag tgtatcagaa cacccgacat cgacaatgta ggtaaaacgg
     2221 caagacacgg cacatatttt gaaatgctcg gcaacttctc atttggtgac tatttcaagc
     2281 atgaggctac tgcctgggcg tgggagtatc tcacaaaggt gcttgagatc cccgaggaca
     2341 gactt
//
LOCUS       DAWAOG010000327         2331 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5946_length_2331_cov_1.855009, whole genome shotgun sequence.
ACCESSION   DAWAOG010000327 DAWAOG010000000
VERSION     DAWAOG010000327.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5946_length_2331_cov_1.855009"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1660..1669
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          657 a          571 c          499 g          594 t
ORIGIN      
        1 ggtaaacgaa agctttatcg acacgattac caccgagctt gcaggaatac tcaactacac
       61 ctcagagcag ggcggcaaga aattcaccga aataacagat aacataaaat cggctatagg
      121 cgacgttatc gacaatcttt ccgcttttca aagctcgctg tcgcttataa gcacaacact
      181 tgacagcgca gatactatgg ttgaaaacgc aaagaaaact atcccgaacc ttgaacagct
      241 gcttgctcag tcgggtacac tgacatcttc cgccaaggac agcataacag cggttcaggc
      301 ggtttcacag cagataacca caagcaccga tatggtctta agctcaatag atgctcttta
      361 cacagaggtt gaaagcgaca tatccggcat tacggaagaa atagccgcta acgccgataa
      421 cgccgcggaa aaggtaagga atatctccaa gataaatcag aagaagatcg acctgctgac
      481 agacctcaga gagcatatag taacagtcgg caataagctc ggcgtaaaag taacgcttct
      541 taccgacaag atagaccgca atatagaaaa gcagaacgct ataatcgaca agctgaacaa
      601 cggggctgat actataagca gtacaggtca cgctcccgac aactttgaaa aggacgtaaa
      661 cacgctgaat aaagaagcgg cagacgaatt tgacggtatc agaagcacct ggaatacatc
      721 ggtcaaatca tcgatcgata ccgccgtaaa tgaagtgttc aagacgcttg acggaatttc
      781 cgcactgctc agcgatgcag gaacatccgt acccgatatt gaaaacgcac tcacagacgc
      841 tcagaattcg cttacaagcc ttaaatcctg ccttaccgac ctcgataaaa taatcggaaa
      901 cgcaaaggat aagctcaccg atatgcagaa aaaggtcgat gatataagca gcgatcaggc
      961 gcttatagct tttatcgaag aggtttcaag tgacccgtca tctctcggcg aatttatgag
     1021 ctcacccgtt gaaattaaca ccgtcaaggt ttatcctgta gaaaattacg gctcggctat
     1081 gacaccattc tacacgatac tcgctatctg ggtaggctcg atcgtacttg tcgccgtact
     1141 gaaagcagag atcagccgcc gtgatattga aagactcggc ggtaatgtaa gaccgataca
     1201 ggcttatttc ggcagatatc tgatatatct tatggtatcg ataatacagg cgataataat
     1261 cgcactcggc gatcttttgt tccttaaagt ccagtgtgaa gacccgctgt tgttcattat
     1321 cgccgccgtt atatcttcta ttgtattctc tttgctcatc tactcgctga ccatcacatt
     1381 cagcgtaata ggaaaggctc ttgcggtaat tatcctggta ctccaggttg caggctcggg
     1441 cggtacattc cctcccgagg ttctccccga atttttccgc aacattcttc cctatctgcc
     1501 attcagatac agcataaacg caatgcgtga agcggtcgca ggcgtatatg cgcaaagcta
     1561 tatagaagac ctgctctacc tgctgatcta cgttgctata gctctcttca tcggaatagt
     1621 gctgagaaaa ccgtgtataa ggataatgaa gttctttaan nnnnnnnnng gggtcacttt
     1681 ctacatattt taagcaaatg ctatagatta tcttttcctg ttttgcactg cttttttcag
     1741 cttcgctctg ccctctttca gtgaggggaa tgccttgtac aaacgcttgt gctttgcttt
     1801 aagttctgac agtctttcgt taagctcatt aaatcgtgcc ataagctctt ctctgcgctc
     1861 cgccgcactg cctttaagct cacttatacg ttcgctgttg cgttgcttta cactgtctga
     1921 ggcggtttcc agccttgctt tcagctcatc ggctttcttc tgcatttcat cgggagtaat
     1981 tccatgctcc tttgaaaatt caactgcctg tgaagtctta tcggacagac ccgagcctat
     2041 ggcatcggat accgctgtga gctgtttttc aagctcatca ataagcctta tctcattcct
     2101 gagcttcttt acctcgggga atgtcagtat aacgtccgtg agtattgccg catagaaaag
     2161 gcagagaatg actatgccga gcggcttcca gataagtccc acagcaaaag ccacagcagg
     2221 ctgaatgacc ttcattatag caaggctggc aagcccccac acaacggtaa actgcgggca
     2281 aatgtatccg ccgatgttta gcgggaaatc ggaatagtcc caccatttat t
//
LOCUS       DAWAOG010000328         2326 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5961_length_2326_cov_1.858653, whole genome shotgun sequence.
ACCESSION   DAWAOG010000328 DAWAOG010000000
VERSION     DAWAOG010000328.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5961_length_2326_cov_1.858653"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          650 a          522 c          478 g          676 t
ORIGIN      
        1 tgatgatact ggcgtttact ggagcgagaa aagggattat tcaaaagaaa ttatgctttt
       61 cttaacctgt tctatgggaa agtccattga aaataaattc tcatatggta agaaattacg
      121 cagttcacaa agctttaagt ttaaaatgca gttacctgta aaaaatgaac agcctgatta
      181 tgagctaatg tcaactctta tctcggcaat acagaaactt gtcattgctg atgttgtaaa
      241 gttcagcgat gagaaaataa aagctacaaa acaagttgta ggataataaa aatcgcaagt
      301 tattcactta gtattgagtg atttaacttg cgattatttt gtatgtcgaa tatagctgtt
      361 tggggtgaag tatacttatt tggtgaccac ttactttaag ataggcgcaa atctgatacg
      421 gatattcggc atctttgtga tagtcgaata agtgttcagc atccagtctt cactgccgtc
      481 atttacattc tcaccgcttg cgggaggtgc gaatatcttg agtgtcatat ccgcttcgcc
      541 gtcaagtggc ttttcaaaga atgccgacat aaaatcgatc gtctttgcgt gaggcgcctt
      601 gtagaaccac ttgccgttga tctccatcat aaggttgagt tctgcgtcct caccgtcaaa
      661 tgtgatctca cagaatgtgg ggttcttctc aaagtgcaga ggtatctcaa taccctcagc
      721 gtcctcagaa ccgccccaac gtaattttac agggaactgt gcgtcaccct cgctcttcat
      781 ttcaggatag aaatactcat cgtgaataat ttccttgtag gtctgcatta ccggctgttc
      841 aataccgcag tcagcattgt tcggatcctc aatttcaagt cccagtctgc ctctgtcacg
      901 gaactgatag aatgtaaagc cgttgaacca gccctgatcc ttgtcgttct tgagcatatc
      961 gcagaattcc ttaaccattt cagcctgctc gtaaggtccc gttacatcgc cgtcagcgtt
     1021 gaactcgctc attaccatag gcttctttac tccgccgcaa agctctctgt aacgcttttc
     1081 cgacagctta gtaagattat atgtatccgc taccttgctt ctgccgaaag agtttccgcc
     1141 ctcgtccgct acgtcaaaag gccagcccca gtgaagagcc atgtacttgt caacagacca
     1201 gatatccgtt gctcttacag catcggcaaa ttccttctcc atctcgattt ctccgccctt
     1261 ttcgagatgc tccacaccgc cgatacagag gatagtctga acgttgggcg caaacttctt
     1321 tattacctca cgggcatgaa tatagaaatc agccacctgc tgatatgtag cacgctttgt
     1381 gaatgaaaac cagttgcctg tagcttcatg gtttattctc agctgcatac gaccgaatgt
     1441 agaaagctcc ttgcctatct gttcaagatg ctcgtcagtt acattggggt cgatcgtcat
     1501 tgtgagtata acgtcctgtc cgtgtcttct tacatcacgc atacaggtga aaggctcatc
     1561 atcggtcgtt ccgccgatac cgcccttgta tgtgaaatag tcatcataag gataatccca
     1621 ctggaaggtg atctccttgt ccttgaaagc atttctcgct cttgtgggcc atgtttcgtc
     1681 aagaggataa tagcaataca taaggcttac cgctctgtga ggtctgccca gcttgcccag
     1741 gatataatcc tgatttacat actctcctct ttcacttccg tcgccaaggt caaccgcgca
     1801 ttttgcaggg cccatatgga tctttttgat ctcgctcatg ttaataaact ccttacacaa
     1861 cttttttctg ttaagaaatt gcgaaaacct ttgttttcac cgaataaatt gagtatatca
     1921 taaaatgaaa acgatttcaa ggctttacaa aaaaattttt ttggtatata ataaaatggt
     1981 attgcaaaat cgaaaggaac ggtaacctat gttagaatat gatgaactca aagttgagtt
     2041 cgagggctat gagcctgagc ttaaagagct tgccggcgca cttgaccttg ataatctcaa
     2101 agcagaaata gcaaaactcg aagaacagag cgcacagcct gatttctgga acgatgtaga
     2161 aaattctcag aaaatatcgc agaagatagg cgaggataag aatatagtac agagctatga
     2221 aaagctccgt gaaagctatg acgatgtaat gacaatgctt gagcttgctc aggaagaaga
     2281 tgacgaatct atgctccccg agatacagga gcttgcaaag catttc
//
LOCUS       DAWAOG010000329         2320 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5984_length_2320_cov_2.664901, whole genome shotgun sequence.
ACCESSION   DAWAOG010000329 DAWAOG010000000
VERSION     DAWAOG010000329.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5984_length_2320_cov_2.664901"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    968..1005
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          503 a          596 c          488 g          695 t
ORIGIN      
        1 cactattttg cgcttcgact cactttttct tttccgggtt gcgtcacaac ccttttatct
       61 actgctgtaa aattatatat tctctgctat cttgtcaccc atctcagagc atgagagctt
      121 agtcataccc tcgctcatga tatcgcctgt acggtagccg tccttaagta ccttgcttac
      181 tgcgttttca actgcgtcag cttccttcac catatcaaag ctgtaacgga gcatcattgc
      241 ggctgaaagt atggtagcta tcgggtttgc cttgttctga cctgcaatgt cgggagctga
      301 accgccgctc ggctcgtaca gaccgaactt tgtttcgttc atacttgcag aggggagcat
      361 accgatagag cctgtgatca tacttgcctc gtctgacagg atatcaccga acatattttc
      421 cgttaccata acatcaaact gcgcagggtc tttaacaagc tgcattgcgc tgttgtcaac
      481 aagcatatgt tcaagagtga cctcgggata atcctttgat acctctgtta cgacctttct
      541 ccacagcctt gaagagtcga gaacgttagc cttatcaacg ctagtcactt tctttcttct
      601 cttcatagca acgtcaaatg cctttattgc aattctgcgg atctcttcct cactgtatgt
      661 aagagtatca gtagctgtca taacgccgtt tacttcttcc gtctttcttg cgccgaagta
      721 aagaccgccg gtaagctcac gcataatgag catatcaaag ccctttgcgc ttatctctgt
      781 ctttaagggg caagcgcctg caagctcagg ataaagcagg cagggacgga ggtttgcaaa
      841 cagtccgagc gcttttctta taccgagcag acctgcctcg ggacgcagat ttgcggggag
      901 cttgtaccag ggtgatgttg tcgtatcacc gccgatagag cccataagca cagcgtccga
      961 tgctttannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtttc gtctgtcagc
     1021 ggtacgccgt tcgcgtcaat tgagcatccg cccataagaa gctgtgtgta gttgaacttg
     1081 tgaccgaact tttcggctac cttgtcgagc accttcatac tctcggtaac tatctcaggt
     1141 cctattccgt cacccttgat aacggctatg ttcttttcca taggtaatat cctttctgtg
     1201 agcttaaagc tctattctgt ataaagcgga tctgtcatcc gctttttcaa agccgaattt
     1261 tacaagaaag cgctctgccg cttcattatc gggtactgcc acagcatact tatatccgca
     1321 ggcgcgcatg gtcctcagcg tgtggttaag aagcttacgg gcaacgccgt aatgtctttt
     1381 atcctcacgg acaaacagcc tttcaagcac gacttcgtcg tttatgctgt cctgcttttc
     1441 cgttgttatg cagacggcac acagctcatc gtccgtgtac agtccgaaac cgtgcagttt
     1501 taccgatata tcgctatagc cctgctcccg cagttcgctg tcggataacg cttttatatc
     1561 taccctcatg acataacgct ttctgcggtc ttacttcttt aaggagttca gcagaccgcc
     1621 cttgttgatt atatccttga taaattcggg gaaaggctca gccttatatg tacagccctt
     1681 tgtcacattt gtgataactc ccgtatcaaa gtcgacctcg acctcgttgc cgttctcaat
     1741 atccttagca gcttcatcgc actctaagat ggcaagtccg atgttgatag cgttgcggta
     1801 gaatattctt gcaaatgtgc ttgcgataac gcaggaaatt ccgcttgcct tgattgcgat
     1861 aggcgcgtgt tctcttgagc ttccgcagcc gaaattcata ttggcaacta taatgtcgcc
     1921 gtccttgaca ttctttacaa aatccttgtc tatgtcctcc atacagtgac ttgcaagctc
     1981 cttgtggtcg gctgtattaa ggtatcttgc aggaatgata acgtcggtat caacgttgtc
     2041 accgtatttg tgtactgtac cgtgtgcctt catatatgtc tttcctttct catatctcaa
     2101 aatctacgca gctgcgtctt gccgtataag gtcttaccgt gctgtcggct accttgacgt
     2161 gctcggggtg ggtggaatag ccctttaacg cttcggcact ttcgaatgtg ctgtccagca
     2221 tcagatctcc agtggaagaa cctaagatat cggtgatcac ctttatctcg ataagtccgt
     2281 ctatcttgcc cgcaaggctt tcaagcccct gctttataag
//
LOCUS       DAWAOG010000330         2318 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_5996_length_2318_cov_2.046840, whole genome shotgun sequence.
ACCESSION   DAWAOG010000330 DAWAOG010000000
VERSION     DAWAOG010000330.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5996_length_2318_cov_2.046840"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    851..860
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          597 a          546 c          486 g          679 t
ORIGIN      
        1 gttcaagtat atcatctccg ctgttgtaat atgaatacat tttacaccct ttagggtata
       61 atgtcaaggc gatttataga ttattatacc ctaaaaggtg cagtttatgc agtcaatatc
      121 aaaattatac cctatagggt gtaatttcag ctgataatga ctgttttata ccttttgggg
      181 tataaaaata cggacggcaa aagccgtccg caccagtttg tagaaaaagt ctgtatttat
      241 aaaaatagag caaattctct atccctttaa gaacgctttg tgggctttgc gtccttgcaa
      301 agcctttggg cgttctatat cggcatcctt gccgaatccc caaacccctt tccccaggaa
      361 aggggctata ttactggggc taacgcccct agtccaagca aggatgcttg cagtcagtcg
      421 cacacagtcg ctgtgcaagg acgcataatg acagccacac aaagccgttg cacgatgcaa
      481 cggcacaaat ggctgtcaat gcacagatgc aacggcacaa atggctgacg acctgtacag
      541 gggcttcgcc ccaaagcaag gatgctttgg ttgcttgcca aaagttttgc acgacgcaaa
      601 accaacagag caagcaataa gcgacacccc atttttaata ttaacaagag gtttttcgac
      661 agactgatac ggacggcaaa agccgtccgc acttatattt acatttcggt gagctgtccg
      721 tctaccgata tgatcgcaac gtgccacgga atgaacttgc cgctgttttt taaagctgtc
      781 ttaacggcat tctcaatgcc tgcacagcac ggaacttcca ttctgactac agttacgctc
      841 tttatatcgt nnnnnnnnnn gtttatcgac agactgattt ttatcttctt atcggattta
      901 tcggaatgtt tctgtcatac ggcgaaacat ggggacttgc gtttacgttt cttacggtgt
      961 atttccgcac taagaaagaa agccgctcat tataacggca tagcgcataa cgagcggcat
     1021 ttgtatttat gaatttatgt cagccttaca tcacacagtt catagcccca aacagcgcca
     1081 tctgcgcaaa atcgttgctt ttactgtcgt tttcggtgct cgcttcttct ttgctttcat
     1141 tttcgcgtga attagctgta ccaaataatc cttgtaatgc ttcaaatacg gcaaacttta
     1201 tctcataatc gcgactttct tcaacgcgcc tgctttcttc ttctgtgaaa aattctcctg
     1261 cggcgggcag gcatagcgga gggaacataa cgcaccacca gtttctgccc tcagcgtttc
     1321 cgataagcaa gcgcactgcg gtatattcgc ctgcgggtaa tgttacattt tcgtactttc
     1381 gggtcgtgaa ataagttcta gcgacctcgc attttacatt gtaatcataa ccctttgaat
     1441 gtacaaattc gtttgctttt tcctcaattt ccgctctgcg ctcgtttgcg acatacaccg
     1501 atttatcaaa gctttcgcag ctgccgaaaa cttccgagaa ctcagtcagc atatagtcac
     1561 gcaagtcgta tttcagcgtc tgatcttcct tactgtccga gtttgcaagt atgtgaaggc
     1621 tgagcacctc ctgcggcttt tcctcacagt cctcggcaaa tgccgtaaag cctgttatta
     1681 taaggctcag caccgcacct atcatcatag ctttatcaag aatattacgt ttaaaaaaac
     1741 ctgtcataag tacctccgta gcattagctt tgtgaggtaa ttattgacag gttgatttcg
     1801 gttattcaaa aatgtgttta tttcaggttc tctatctgac ttagccataa cgttgcgcag
     1861 gcatcgctcg gcattctcca gtcgcctctg ggtgacagcg ttaccgaacc taccttagga
     1921 ccgtccggca gacacgaacg cttgaactgt tgtgagaaga agcgacggta gaaggttttg
     1981 agccatttga gtatagttac acggtcatat actccatcaa acgtatacag cgcaagtctg
     2041 aacacctttt cgggtgtaaa tccccatctg atgccgttat ataagaagaa atcgtgtagc
     2101 tcgtaaggac ctacaaggtc ttcggtcttc tgagaaattt catcgccgtt tgcaggcaga
     2161 agctcggggc ttaccggggt gtcaagtata tcctcaagtg attccttaag cagcttatta
     2221 cctgtcgtct tgctgtagta gcttacaagg tgcttgatta gtgttttagg gatcgagcag
     2281 tttacgccat acatcgacat atgatcgccg ttgtaggt
//
LOCUS       DAWAOG010000331         2312 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6018_length_2312_cov_2.205140, whole genome shotgun sequence.
ACCESSION   DAWAOG010000331 DAWAOG010000000
VERSION     DAWAOG010000331.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6018_length_2312_cov_2.205140"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          558 a          599 c          476 g          679 t
ORIGIN      
        1 tacataacct ctttcctttt aaaatacaaa aaaggcgcaa ctcacctcac atgaactatg
       61 ccttaataac gatatttaat tttgcgcaga gatattgtgc attacttaca gaggtgcttt
      121 tatttagcct caatttacac aaattggtat ttaccgcctt gcacatatct ctcacctttt
      181 catttcttaa gcttaatctt tatgctttat acagcataag catatcgcta tagttttatt
      241 ttgtcaagca gtgtcgctcg gtaaaacacc accgttttgc tgtttttaac aaaccgcaca
      301 attttcatag gcggaattag cagataaatt gcagcccgcc gttccgttat cctcggaaaa
      361 ccgaaaacat tcacaaagac agaacttgga tcggcacact gccgccggca ctcgccggta
      421 gcgttaccta cgccaacatt cggaacggca aatttacagt ggctttcagc cacacggcga
      481 atttggagcg gcacattgcc gttgtaaccg ccttatcata ttgcatattg ctttatgtaa
      541 ccccgccgtt ccactatcct cggaaaactg acagcattcc caaaggcgga attgtcagcg
      601 gcgacccgcc gcttaggggt ggggtttgcc ttcgcgatag gcgttcatta tatcgcggaa
      661 cacttcgccg tttgtcggcg gtgtccatgt gatcgcatta gcccccgctt ctattgtcgc
      721 gagtatagac tccctggtag gacctccggt cgcgattatc ggaatatcgg ggtgacgctt
      781 gcgtatttcc tttacaacat tcgttgtctt gcccgccgca gatacgttga atatctttgc
      841 acccgcttca agcctgctct cgatgtcctc gtgctcagat attaccgtga caacaacagg
      901 gatatcgatc gtgtcgcata cctgacgcac aacatcatta gatgtcggtg cattgactac
      961 aacaccgata gcaccctgaa attccgcgtg catagagaga tttaccacgc gctttccctg
     1021 cgtaagcccg ccgcctacac cggtgaaaac aggtatatcc gccgcgctca ttatagtctg
     1081 cgtgattata ggctgcggcg taaacggata taccgctatt accgcatcag cattgacatt
     1141 tcttataatg cagatgtcag ttgtaaatac tattgatttt ataagtttgc ccattatcct
     1201 tataccgctt gcccgctgta tttcctcggg tactttgagc ataaagcttc tcagcggacc
     1261 gctgatttca ggtatgtaca cattgtctgc cattattcgt atctcctttc agtttgacct
     1321 cagacgtatt cgcccccgat tcctcttacg cgcacaagtc ccgagttcac ggtaaattca
     1381 ccgttctcgt tctcgcagat aaggcagccg tccggagcga tagcctttgc atatgctgtt
     1441 gtttctttat tgttctgcac cagtttcact gttttcccga gcgttacaca gcggtcggaa
     1501 tattctttta tcaccgccga gaaatcagca tcgataagcg tttcaagctc tgcaacaata
     1561 taatctgccg caagcatttt atcatactta ttgcccgtca gcgtgtaaag tgatgccgca
     1621 ttgacgagtc cgttgctctc aaagaagtcg ttgctttgat tgacattcag tcccattccg
     1681 cagaccacgt tgttataaat tgcgccgtcc gaggaacatt tgaccgagct ttcgcataaa
     1741 ataccacata ccttgcgttc agagcagatt atgtcattag tccactttat gcccgccttt
     1801 atgccactca gccgctcaac ggcgcggcat acggctaccg cacatattac cgtaagatca
     1861 tgaccgtgtt gcggcgattt tatcaatatc gatatcggaa gcatatctcg tgtcgcaagc
     1921 catgtattgc caattctgcc cttgcctctg ctctgcatat cggcaataac taccgtcctg
     1981 tccgggagct catcggcgtg ttcctttata tatgtattcg ttgacggtat tgtatcaaag
     2041 ataattaaag gacagccgct tatattactg cttatctgca tggtaatcaa gcgccactat
     2101 attcatcgtg atcttcttat tgtcatcaat tgttatgata ccgtatgacg gcgggcgctt
     2161 atctctggga gaatcgatac tgccgggatt cataacatat ataccgtcaa taagctcggt
     2221 gcgataaagg tgcgtatgac cgtaaagcgc gatcttgcaa ccgttctgct ttgcaacgga
     2281 aatgagcgta tcaagtccct gatgtacatg gt
//
LOCUS       DAWAOG010000332         2297 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6079_length_2297_cov_1.796610, whole genome shotgun sequence.
ACCESSION   DAWAOG010000332 DAWAOG010000000
VERSION     DAWAOG010000332.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6079_length_2297_cov_1.796610"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1424..1433
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2017..2026
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          670 a          454 c          496 g          657 t
ORIGIN      
        1 gcattatgga ggacgtgcag acgatactgg cggcggggtg cggcgcttct acaaagctgt
       61 atgacggtaa aaacgtttcg cgcgttataa attacaagta tccttacgaa tacatttcgc
      121 ggtttgagct tatgaacgag cgtaaacgtg atatagagaa tttctttgaa agcaaattaa
      181 cgcttgcata attttactta ttgtgctaca atataaaaaa gcatttctga aaggaaacac
      241 ggctatggat ttaaaagatg aactggattt tataacagca aaggcaaaac agcttacaga
      301 tacaggtatc cgcaaggctg acgaagttct cagcatatca aagctgaaat tgagatgtgt
      361 tcagcttgac acgatgataa aagctaagta tacagagctt ggcagaacaa tgtacggtat
      421 ggtgaaaaac gacagctctg atgccgacag aatagcacag gctgtaatgg agatagatca
      481 gctctataag aagatggcac aatacaatgc aaggatagag atgatgagaa aaattgttac
      541 ttgtcctgtt tgcggaacaa aaaacaaatt cagcaatact tattgtacaa gttgtatgca
      601 taaacttgtt ttgactgatg aagaacctga ggactatgcg tatgctccca atgaagcgga
      661 gaacgagtaa atttgatttg ttaattggca cggctcaaag ggtcgtgctt ttttcttgct
      721 ttgtttttga tatagaaagc ccccgcgcag atcctgcgcg ggggtattcg tctgcttaat
      781 tctgacagtt gattatttct tctgttatca taggataaga gataagctca ttaccgtcaa
      841 ggctttcctt tctcatatca actgccgata ttctgtgatt gtcgtaggtc gtataatagt
      901 atattccttt atcggcgtta caacaggagg tgtatattgt tatctcatat ttgccatcag
      961 caacctcgca acagcctctc ggctgatcga cagagccgag gatatggaag aactggctga
     1021 cgctttcggt ttctcctttt cctgaaaccg agttagcttt cacaaaagct acgcgcacaa
     1081 atcttgactg tgacgaaaga tcccccggaa gtccgatagc gcccatacct ctgctgtatg
     1141 ctttaagctc aagcttatcg gaaaatgtgt tcttcggctg tcttgacgat agatgcatat
     1201 agtcgttcag cctgaacatc tgctcatcaa acggcggatt gttcgtcagc acgccgagag
     1261 ggttatcata taccttaatc ccatcagata cagattcaac cgtgatcgca ctgtttttgt
     1321 cagctattat ccagtggagc tgtcctaccg gcatatgttc gctgaaagga gtgtcggtaa
     1381 tgtttatatt tgaaataagc tctttagctt catcagtagt tgtnnnnnnn nnnagtatcc
     1441 acggtataaa ttcgtactga gctatattgc tttttccgtt tttatttttg aaatagtggg
     1501 cgtttccgac aaagttgagt cctgccatac acaacccctt ttcattcacg gcatcataga
     1561 aaagtggata tccgccagcg atatgggcag tgccgataac ggcgtaatgc ttttcgcaga
     1621 taccctcact gagaaagtcg agcctgaaat ttctcggcat tataacgatc tcctcgccgt
     1681 atgagctttc gtaatcaagc gttcttccaa agtaaaaatc atttgtttta tatgttgcgg
     1741 ctgtgcacat agttttacct ccgtttttgg tttctgatct tattctacta cattttctca
     1801 cataagtaaa ggaatattac tgcacagaaa aagcagaacg aatttttttt caatacggat
     1861 tttgagaaat tcatgaatat ttccgccgtt actcacatat atcagagcat acacgcagaa
     1921 aatgcactac ttgaccataa gcgtgacagc atgaataagg taggcgagat attttatagc
     1981 ggaattgcgt tttagaaatg ccgagaaccc tgataannnn nnnnnntcac agccgtagat
     2041 ccgctctgca agcgggttta gctgaagcta gaaaataatt ttcaaaaaaa cgcttgacaa
     2101 acattaaact tcgtgttata atatacaagc actcaggaga gtgaacagaa tatcgcgggg
     2161 tggagcaggg gtagctcgtc gggctcataa cccgaaggtc gttggttcga atccagctcc
     2221 cgcaaccata taaaagcact tagcgtaatg ctaggtactt ttttaaatga agcgcacctg
     2281 acggtgcatg aaaaatg
//
LOCUS       DAWAOG010000333         2296 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6082_length_2296_cov_1.890674, whole genome shotgun sequence.
ACCESSION   DAWAOG010000333 DAWAOG010000000
VERSION     DAWAOG010000333.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6082_length_2296_cov_1.890674"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1878..1958
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          568 a          468 c          412 g          767 t
ORIGIN      
        1 tatctgtgct gcggattcca ttccataagg ctgacgatct tcgcaaagaa aatcctgcaa
       61 gtaatgggac gtggcttgcg cttggaattg caccacagaa atgaatcctt ttcatcttcc
      121 gtgcaagctt ttacggcaat tttcttttgt tcggcattta caagaaactg aatataactc
      181 acattgggca atttcttcat acatgccgta ttcagtgtta ctttacagtt gttaaagctt
      241 atggaaggct ctgaaatatg ggcgaaaaat tcgcttcgta caacctgata cccgtcatag
      301 ctgaatgagt catccttaat tatatcagcc tcattgttat agcacggaag ctgaggagcg
      361 tttgtcattg tagtaagctg aatattatcc atcgttgttt tcctccatct ttatattatt
      421 gataagggtc agtatattat ctcccacttc ttctctgctt gtgacattca ggtcaaggta
      481 atcggaaaat gatgttattt cctgctgcat attccaatct tgttcctttt tcatcctttg
      541 cagttcttct gcctgtgaat gatgataata attactgccg aaattatctg cccacgctga
      601 cggatatgca ataaccgatt taccagtttc ggcaagagag gttactttat catcatcggt
      661 acagagtccg ttatccttcg gcaaatataa ttctgtttct tcaaggttga atataatcaa
      721 attttcatcg gcattttgtt ttcgtattcc gcggacacga tatttacaac tttcattcca
      781 accgagcagg ttgtaaatcg tttttaaaaa cgccgtaccg cttacgggtc gtgagatgaa
      841 ttttcttttt tcgttgagaa acaaccattt catagcgttc ttatttttgc tgtcagctgc
      901 acggactatc aacagctttt ttaacgggtg aatgagaagt tctataaatt gtgtgtcggg
      961 gagttttttt acgcagttat agctgaactt cattgatttc aaggagaatg taacagatag
     1021 cttatcggaa ttgttaaaaa actgctctct cgcaatttca aaacctctgt aatcaaaatc
     1081 tccgccattc gcttcaacct gaaaggtacc gttttttctt tgttcatctt cgttgcaaac
     1141 agttttagca atgtaaagat aatcgtcaac tttaaaaccc gcccatcgag gatttatgga
     1201 gataaagccc ttcaatgtgc cttcgggtat aactttcaac tcaggaagaa tacccttgtt
     1261 tccgtatttt gcattgttta taagatgctg aacagcaata aaatcatctt tggaaataat
     1321 cggctcatgt tcgcctttcc atcggtgctg agctcgttcc ccacggtttt tccttgattt
     1381 atgatccaaa tagcttggcg tataggtttt tctggtgagt atttcgccgc aatagcgttc
     1441 gtttctcaat atctccagaa cactcccggc gttccatatc gtatttcctt tctttgtccg
     1501 ccttccgagt tcagtcagag aatttgctat atccttacaa gaatagccat acagatacat
     1561 aaaaaatatg agccttactg ttttggcttc gctttcattg atagtcagtt ttccgtcctc
     1621 attgtggtcg taaccgagca gaacgggtgt tagcacaata cctctaccga aacgcatttc
     1681 aacagaggcg ttcataatgg agctttttat atgtgactcc tcctgagcta ttgttgcggt
     1741 gaagctaagg ctcatttctg tgttgtcgtt cagggtataa ataccttctg tttcaaatcg
     1801 tacgcctacg ggatttttta aacaggaaag ctgtcttacg atgcttatgc aatctacgat
     1861 atttctagca aaacgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg ctgtaaagaa gtgccggata
     1981 taccttcatc tgcatatata cctacgaatg tccaatatgg atttttactg actttttctt
     2041 catagtagtt cttctgcagt tcatacgagc tggtttgttg cagattatcg gtagatactc
     2101 ttacatatac agctacacgc ctttcaatgt cgtcacaata atcagccgct ttctttcccg
     2161 gaattacttc aagcaattca gaatttgagc ctttgtagcg ttccctgatt ttttcgcgtt
     2221 gcaattcttt atttgaaacg gttgcttcac tttcaagcat agctatcacc tcagcgttgt
     2281 attgtatatc cagtat
//
LOCUS       DAWAOG010000334         2292 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6098_length_2292_cov_2.589629, whole genome shotgun sequence.
ACCESSION   DAWAOG010000334 DAWAOG010000000
VERSION     DAWAOG010000334.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6098_length_2292_cov_2.589629"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    252..261
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1881..1890
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          653 a          465 c          534 g          620 t
ORIGIN      
        1 cacctttcct tttacgggtt gtgtcacaac cccttttttg ccgcattttc ccgtatgtcg
       61 tctaaaaata acgcgaaaat gttgtgaaag tttacaaaaa cgcttgattt atgttttgtt
      121 atatggtaaa attaaataaa atcataatgg cggtactatg gatatatgta gaggtagtca
      181 agccttatga tttaccgtgt tacctcgctt taacagttta tcttgttaaa ctatcatact
      241 tcatattagc cnnnnnnnnn nccgaggagg cgaaaacggt gcagcttaac accaacgacc
      301 agaagttcat aaagataata atagggtttt caagagattt tccctttacg ataaatccgg
      361 gagaagcggc ggagcagaca tatccgtcac ttctggagct tataaatccc gatgatatcc
      421 cgccgataac cgagatgttc accgacgtat ctcaggacga ggaaaagcgt tttgacgcac
      481 attgccggtt tatggtgaat gatgtatatc actggttttt tctctcatgc aaggcagtct
      541 atgccgaata cggcagaccg ataaggtttg aggggactat gtgcgatgtt tccacatatc
      601 ttgaaaccgc aggcgacgac cttgtgtata atgaattcag gaaaaagcat aatatacgac
      661 tgacggaact taaaaaggga gcgccggggc ttgcagatgt gcttgatgtt gattatctca
      721 cgagcattca gcgtccgttt gcgcagcctc agctctattc ggcaattttt gacgaaaaag
      781 gcaagctgat atgtattccg aagacacagc acaagtcgct ttctcctgac gactttgcgt
      841 atcagaagaa aaagaatatc cgcataaatc atctcgtcag cggtacatgg gtactcggtg
      901 caaagacgca ggaattgctt gatgataaca ttcagctttg ggaaacgctt gtacagacgg
      961 tttcccgtat ggcgaatgct tttgtagtcg ttttgtcgga aatggataac tcgcagaatg
     1021 caaataagct gctcggtcag aatgtcgaag aacagatcct gcttaataat atctatgcaa
     1081 taattatgga aagcaagagt gcggatatag cacttaattc agttatggaa cttatcggtg
     1141 attacttcca tcttgacagg ataactatag ttgaccgtgt tgactttaca caggagctgt
     1201 gctggtgcag ggactacaag tacagaagat taccgcttga aatagacgga gaaacggcgc
     1261 ttggctgcgg ttcgatacga gaggaactca gccttacatc ttcagcgttt tcggacgcgg
     1321 atacaaacga ccttgagaag tacggtataa agtcttatgc tatcttcaag ctgataaatt
     1381 cgggttcgtt tgacagcctt ataatgttcc agatgatcga aaaagaacac aactggacac
     1441 agcgggagcg caaacagctc cgcaacattt cacaaatcat ttcatcgctt atgatgcgta
     1501 aggaaacgca ggataagctt gaacagtcgc agaagcgtat gcgtcagctt gcgttctatg
     1561 acgctatcta caatattcct aaccgcgcaa ggctgaataa agatcttgac aagataatga
     1621 agcataacgg taaaggctca ctgttggctt ttaaagtcac aaatacccgc tcgctgtcgg
     1681 ctgtatacgg acacacatat tcggatatgc tgctccgcag tatagcacag tatcttggaa
     1741 atttgcctat caaggacata ggcgtgtatt attttacaaa tgctatattt atgctaaatc
     1801 tccccgactg tacggataat gaggcgaaaa acctggcgga aatgctgata tatcgctttt
     1861 caaagccgtg gaaattcggt nnnnnnnnnn ctctatccat tgcagtcttg gcatagcatt
     1921 ctatcccgaa aacggagaag atgccgagga gctgtgcaag gcggcaagta ccgctatgta
     1981 tcgtgcaagg gagtttaagc aaaacagcta tgctttctac tcgggttcgc ttgagcgcac
     2041 aagactgttc gcggcaaatc tcgaacagca cataagagaa tgtataaatg caggaatgag
     2101 gggattttca cttcgttttc agccgtgctt cagtgcggag gacggctgta taacaggctg
     2161 tgaaacgttt gtccgttggc atgatgagca gtatggcaat atacctaatt cgactctttt
     2221 cccgatggct gaaaacctcg gcttatctca tgttattgac ggttgggtaa tggagcgttc
     2281 gtgcgagttt tg
//
LOCUS       DAWAOG010000335         2289 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6107_length_2289_cov_1.684870, whole genome shotgun sequence.
ACCESSION   DAWAOG010000335 DAWAOG010000000
VERSION     DAWAOG010000335.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6107_length_2289_cov_1.684870"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          556 a          572 c          446 g          715 t
ORIGIN      
        1 agattacccg tataggtgtg attgctgagt attcgtgaaa ctgcggactg attccattta
       61 ccgccaaacc gtgacggaac accctcctca ttcaagcgtt ttgcaataag ctggtagccg
      121 ctgcccgaaa ggtattcatt ataaatacgg cggacgattt cggcttccct cggaacaata
      181 acataatgct cgttttccat acgatacccc attaaagtcc tatcccaggg aataccggat
      241 tcaaaactac gcttgattcg ccacttctga ttttcgcttg ccgagcggct ttcttcctgt
      301 gcgtatgaag caagaatcgt catcatcaat tctccgtcac cgctaattgt gtgaatattc
      361 tgctcctcaa aataaatatc cacccccaga gctttgaact cacgaactgt ctgtagcaaa
      421 gtgactgtat ttcgtgcaaa gcgggagatg gattttgtaa tcaccatatc aattttaccg
      481 gcacggcagt ctgcaacaag actttgaaat cctgctcttg aatcctttgt gccggtcttg
      541 gcttcatctg catatactcc tgcataaagc cattcgccgt ggctttggat aaggtcgctg
      601 taatagctga cctgtgctga cagcgagtga agcatagcgt ctttcccact ggaaacacgg
      661 gcataagcgg caactctctt tttccgctcc aattttggtg gttttgatac gatggttatt
      721 tttcttgaca ttacgtcacc tccttgcagt gtgacatatt acctctaaaa ccatcatata
      781 tcaagtcgat tccgcaatac aaactacacg aagatatgcc gtatttttcg gtcattattg
      841 tatcaatagc ggcgtaatca tcagaggtta aaatccccat cgacatcatc tgttttgcct
      901 ggagcattga agcaaggtag ctttccaacc gatttctgta attgtcactc atcagaagta
      961 tgcccctttc tgaaacggtc tgttatgtag caggcatgag aacagtattt tctgtgattg
     1021 tttccgtaag ctgtgaaatg acgtccgcag caggcacagg tatattcata atttgctttt
     1081 cggttgacgc aatccctgtg ttcattccac catgtatttc gacaggaatc ggagcagaac
     1141 tttttcggtt tctgcttagc aatgtttttt atcagctttc cgcactgttt acaggccacg
     1201 gcattctttg atttgtcacc cagaccgttg cggcggcaga atgagcgcac cgtattatct
     1261 gttatgtcga gtgcttttcc tattttaata taaccgacgc cttgtaagcg cattgttcgt
     1321 atttgttctt tttgctgatt tgtcattgag ctttcctcca atcctaggga ttacctcaat
     1381 atctactgga gaaaaaatag tcatccggtc cgaaaaaaat aatgcccacc aaggaaatca
     1441 atccaaggtg ggcattatca ctgtagttgt taagttttat ctgagcattt catttacacg
     1501 tttctgcacc tccgaataat catacccggc ttcagtcaac ttttcctttc gttcggctcc
     1561 attcccccac agacccgcaa taacctcaac tgcaatctca tcggtagttt tcctgtatag
     1621 cttttcattt actcttttct gaatcgtatt gtagtcgtat cctgcggcgg tcagtaatct
     1681 ataacgctct gcaccgttat cccacttgcc ttcaagcacc tcgtcagcaa gctgatctat
     1741 ggttttcttg acaggatttt tcttgccgta atcggtaaag cagatatcac cgtctacatc
     1801 ataaccgctt attctgtcaa gtccccactg ccacatagtc tgaccatagt tgttctttga
     1861 cgggcagtcg gggctgttag tccagtgagc aagccatatg tcgtacttgc cgacaattct
     1921 gctcttgtcg taatagttct ccataaagga cggatttaca taaactccgg gcttgaatcc
     1981 cgcctgtctt atcttttcac agaaagcaat tgccatcttt gtgcgtgtgt ccgtattcag
     2041 accgctgatc tgctttttct cctccatgtc gaagaatacg ggatatgtcg gagataaggt
     2101 cttgattacc tcaatgcact tttcagcctc tgtcagtgcc tgctcaacgc tcattgcata
     2161 gctgtaccag taaaatccgt agtcaatgcc atatttcttg cagtccgcca cgaacttatc
     2221 catagtaaca tctttcttgg tggaaaaacc cgcacggata atcgcaaatc tcacacccgc
     2281 ctgtttcag
//
LOCUS       DAWAOG010000336         2286 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6117_length_2286_cov_1.984312, whole genome shotgun sequence.
ACCESSION   DAWAOG010000336 DAWAOG010000000
VERSION     DAWAOG010000336.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6117_length_2286_cov_1.984312"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          768 a          495 c          460 g          563 t
ORIGIN      
        1 ggataggaaa caacgcattt gaaaaatcaa gtgcaaaaga aatagttata ccggacagcg
       61 tgactgatat cgaacagtat gcttttgagg gctgcacatc actgtcggat ataacactgc
      121 ccgacagcct gacaagcatc ggattaagcg ctttcgaaag ctgcacggca ctaacggata
      181 tagcactgcc cgacagcctc acatatatag acggctttgc atttgcaaac tgcacttcgc
      241 tcaaaacagt tactgtaccg gcaagtgtaa catttatagg cggctgggct ttcggatatt
      301 attttgaaga tggtaacata gatgcaagag tggcattcga tgatttcacg ataaactata
      361 ccaagcacac agcgggacat ttgtatgccg ttgaaaacgg ctttaccgat gaaggttgtc
      421 ttgaatacac agtaaacgct gacggcactt tagaaataga caaatatgtc ggcaacgata
      481 cgatatttga aattcctgcg gaaattggcg gaaaaaaagt cacaagcatc ggatataagg
      541 catttgagaa tcgcacatcg ctcacaagcg tcactatacc cgacagtgtg acagatatcg
      601 gttttcaggc attccataac tgtacatcgc ttaaatcggt tacaattcct gcaagtgtaa
      661 caactataga caactatgct ttcggatatt tttatgacgc aaacaaagaa acaaaaatcg
      721 acggtttcac aataaactat accaagaaca cagcgggaca tttatatgcc gcacaaaacg
      781 gttttaccga tgaagcttgt cttattaacg aattacagga tgacggcact gtaataataa
      841 ccaaatatgc cggcaacgat actgtatttg aaattccttc ggaaattgac ggtaaaaagg
      901 tcacaggtat aggaaacaga gcattccaga acagcacatc gctcacaaat gcaaccatac
      961 ccgacggcgt aacgcatatc gacgaatgcg ctttccaagg ttgctcgtca ctgacagata
     1021 tagcactgcc cgacagtctg gcaattatag actcttacgc tttcaacaac tgcgctatgc
     1081 tcacaagtat aaccatacca aacggcgtaa cacgcatcga atgggcaact ttctacggtt
     1141 gctcggcact gacagatctg gtactgcccg acagcgtcac atatataggt tacaaagtat
     1201 ttttcgactg tccttcactt aaaacggtta caattccggc aagtgtaaca gatatcggtg
     1261 agtatgcttt cggatatcat gatgatgaca attggaatct tgcaaaaata gacggcttca
     1321 aaataaacta cacaaaaaac acatggggtc attattacgc aaagcaaaac ggcttctccg
     1381 acgaaccctg ccttgttgcc gaattaaatg aagacggcac aactttaaag ataagtaaat
     1441 atgccggcaa ccataccgaa tatgtaattc cctcggaaat tgacggcaaa aaagtcacaa
     1501 gtatcggaaa tgatgcattc agaggcaata catcactcat tagcgtcacc ataccgggca
     1561 gcataaagga cataggcgat aatgcttttt cggattgctc gtcgcttgaa agcgtaacta
     1621 tatcggacgg tgttgaacgt atactttatc gggcattcta taactgcact tcactcaaaa
     1681 cagttactgt tccggcaagt gtaacatata tagataataa cgccctcgga tattatagcg
     1741 atgatggcgg aattaatata aataagatcg acggcttcac gataaactat accaagtaca
     1801 cacatggaca tttgtatgcc actgcaaacg gctttaccga tgaaggttat ctttatttca
     1861 atatactgga tgacgacact gtagaaataa agagatatat cggcaacgat acggtatttg
     1921 aaattcctgc cgaaatagac ggcaaacaaa tcacaagcat cggaaattat gcattcctgg
     1981 accgcaaatc gctcacaagc gtcactatac ccgacagtgt gacaattatc cgttttcagg
     2041 cattccataa ctgtccttcg ctcaaaacag ttacaattcc tgcgagtgta acaactatag
     2101 gtgactacgc tttcggatat tttgatgatg aatatcaaaa aacgaaaatt gacggtttca
     2161 aaataaacta taccaagaac acagcaggac ataggtatgc ctttgagaac ggttttaccg
     2221 atgaaacctg tcttcttaca gaaatacagg aagacggcac tgtaaaaata accaaatatt
     2281 gcggca
//
LOCUS       DAWAOG010000337         2268 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6174_length_2268_cov_2.164031, whole genome shotgun sequence.
ACCESSION   DAWAOG010000337 DAWAOG010000000
VERSION     DAWAOG010000337.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6174_length_2268_cov_2.164031"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1764..1773
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          613 a          538 c          478 g          629 t
ORIGIN      
        1 gaatcgcctg ttgtaggagc atctgtagcg ggagcatctg tatcagcggg agctgttgta
       61 tcttcttcac cgccgccgag acctgaaact gtaaattcga tctctaaaga ctttgtgggg
      121 tatgcacctg catagctctc aagatccttg ttccagatgt tagcgaacag aacctttgta
      181 tggcctgact tcttaccgtc ttctgtatca atgataggat cagcaacttc tgatgttact
      241 gtaccatcaa cgatgatctt gcagtcagag aacttagcga tagatgcgcc gtcttcgcct
      301 gcgtccttat caaaaggaat atctgttgaa acaaacagaa ggttgaatgc agaagaaccg
      361 tcgagtgccc agtcaaagtc agtaacgcca accttgtatg tgccatcctt gtcgatcttt
      421 acatctgtgt atgtagcagg aagaacatag ccggcttgac cactcggcat atcatcatcg
      481 aatttgtctt catactgagg gaatgtttca gggtcgttac cgccccatac gattacgctg
      541 ttgaagtact tgccctctgc aacgtcctta ccatatgtag cgtcatcaaa agagtttctg
      601 aatgagtagg gtgctgtctg gaaaccgata taagcattat aagtttctgc tgctgcacct
      661 actgtaagtg tgcctgctac tgccattgca gctgctgcac cgatgaatct tttcatcttc
      721 attttaatta cctccaaaca aattgtatat gtttcattct tatgagtgtt gttaaacgtt
      781 tacaacattc tcaattaagt taattataac ctctgcccgc caaaaaatca atttgcattt
      841 ttcacacatt taccacacaa cttttagtta ttttgtacaa ttaggttatt aagttaaaac
      901 acagaccgtt ctcaattgtc acctatttgt gcagttcagt tgacagtgac ggcaggcggt
      961 ggtatcctgt tatagtcaat tcgacacaag gaaaggaaac aaaaatatga acagacagaa
     1021 aatcacaacc atcgtactat gcgcgctctt tgcggcaatc acggctatac tttcacagat
     1081 agcgatccca atgccctccg gcgtaccggt tacattacag acctttgcgg tagctctctg
     1141 cggcttttat ctctgcacgg caagggcaac cgtatcaacg ctcgtatacg ttctgctcgg
     1201 cgcggtaggc gttccggtat tcagcggctt caagggcgga atcgcatcaa tagtaggccc
     1261 tacgggcgga tttataatcg gctttatcgc tatggcggca atcagcggaa tatccttcag
     1321 aaatatgcct gagaaattcc gtgtaccgct gagaatagca atgggtatcc ttggcgttgc
     1381 cgcttgccat atctgcggat ttgtacagta tgcgcttgta gcgggcaagg acttattatc
     1441 gagcttcctg cttgtatccg ctccctatct tataaaggat atagcaagcg tagtactcgc
     1501 atactttgcc gctctcgcaa tctcaaaggc tctgaaaaga acaggctttg ctccaagcga
     1561 acaggcgtaa ttgcaataat ttcagaaaca ttaaaggcag tgccgattgg cgctgtcttt
     1621 ttgtttttat aataacaagt acaaagtaaa aaaccaattt actgctccct cgtaaaaggt
     1681 aatattgaca tataaatctg aacaattaaa accatacacg ttttaatacc cgccgataac
     1741 gcaagcctcg ccaacctcag acannnnnnn nnnccgtttt gccacgctca gccaacctca
     1801 aacatttcta aaggcggaat ttacagtggc tttcagccac ccgccctgct ttgacttttt
     1861 atgatttaag tgctatactc tataaataga aagacaaaac acggagatac tttaaatgga
     1921 taacaaatac gaagaacggc tcggcacagc ctcgatgctg ccacttatac ttaaaatggc
     1981 actgccgggc tttgcggcac agcttataaa cctgctgtac agcatagttg acagggtctt
     2041 catcggacat atagagatta tcggcactga tgcactggca ggtatcggta ttacaagctc
     2101 aataatcatt cttatctcgg cattttcaca gatagtcggc ggtggcggcg caccgctcgc
     2161 ctcaatagcg ctcggcaagg gcgaccgtga aagagcgcac aggatactcg gcaacggctt
     2221 ttcactgctc gtgctgttta ctgtagtaac atcgggcata acctacat
//
LOCUS       DAWAOG010000338         2263 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6199_length_2263_cov_2.046196, whole genome shotgun sequence.
ACCESSION   DAWAOG010000338 DAWAOG010000000
VERSION     DAWAOG010000338.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6199_length_2263_cov_2.046196"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1026..1035
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          665 a          493 c          419 g          676 t
ORIGIN      
        1 gactaaaggg gctgtatcac agcccctttt aatatctgta ttttaaacct gccgcatatc
       61 gcccttttta tcagacagaa gcggctttat aagcctgtat atctcacctg cacgggtagt
      121 gccgtatttc ttgactatac ggttatttat acctgacttt gtggaaagct catttatgca
      181 ttttcccaca aattcacgtt cgtcactgtc gggtattagc ttttcaagct cagaatatat
      241 tctgtcgtaa tgctcatttc cctttgcctt gtcaagagaa accgcagatg taacattaac
      301 acccttctcc tgccagaatt tcgatacaag ttcaaatgcc ttgtcgtttg aaacaagaca
      361 gtaattttct tccggctttt ctctgagcat atatccgaga taagaaacaa gctgaaaatc
      421 aagcgcgtac ttcataccga cctgtgcctc aacataatag atcttggcac ggctctcgat
      481 aattttcttg tgcatattga aagtgagcac acctgcgtgc tcactgtaaa aaatatagca
      541 ctcatcttta cggtcaagct gttctatgcc tttgagtccg tctgaatgca cattttcaaa
      601 gtctattaga tatatagcca tactgcctct tactgtctgt tacttcaaat tctgcatatt
      661 tgtaacggtc tgtatatacc tgtcatactt attgcagact gtatccacat ctccgaacgc
      721 tatctgcttg cccttgtcaa gccacagcac cttgttgcac agtcttctga cctgcggaag
      781 agagtgcgaa acaaaaagcg tgcagcagcc gttctttatt atattcatca tagtggcttc
      841 gctctttgct ctgaatgccg cgtcaccgac cgacaggacc tcatcgagta tcaatatctc
      901 aggctccata aggcaggata tcgcaaatgc aagtcttgac ttcataccgg aagaaagctg
      961 cttatatgcc ctgtcttcaa attcggtaag ctccgcatat tcaagtatgc tcttgtactt
     1021 tttatnnnnn nnnnnaattc tcttgtataa ccgagcatcg cacctctgag atatatattt
     1081 tctttaagcg taaggtcacc gtcaaaccct gagccgagtt caagcagtgc ggcgatcttg
     1141 ccgtttacgg taacacttcc ttttgcaggc tttataatac ctgatataac cttgagcaaa
     1201 gttgatttgc ctgcgccgtt agtaccgatt atgccaagga aatcaccttc ctcaaggctg
     1261 aaagaaacat tgtctactgc cgtaaatcgt tctgctttta tatctctctt gatctttttg
     1321 ataagatatt ctttaagtcc gatctcacga aaattgttga ctgtatattg aaccgtaatg
     1381 ttctttacat caataaccat ttgcattcct catatcaata ataatataca aacttttgtt
     1441 ctgttttttt atggataaaa atgccgagtc ccagcattcc gagcgaaaat gaaagacata
     1501 taaggtgatg cgacaaactc ggaattgccg actgcataac aagctctctc atataggata
     1561 tatactgata tatcgggttt atgcggaata tgaactgcat attgtccggg aacgacctga
     1621 tatcatagaa aagcgccgaa agataagtga gcaacatagt gaatatctga tagagatatt
     1681 gtatatcctt aaagaacacg aaaagagtcg aaagaatata gctcatacca atattgaata
     1741 ttatcagaca aatcatagga tacagcatca tgaaaagatg aaaaccgaaa tttataccgc
     1801 aagctatcat gaatacaaag taaacgacta atgtcagcag aaagttgaaa gttgcggcag
     1861 agttcgacga aagtacaaac aacgccttcg gaacttttac ctttgaaagt attgagccgt
     1921 ttgagtacat cgaaaacatt cccgccatgg tcgcattgct gaaaaatgta tacataagcg
     1981 tacctgtaag cagatacaca acgaagcccg aatcaaggcg gttaaaaatg tagccgaaca
     2041 caaaatactg gatcataaat gtcaggaacg gtgaaattac gctccagagt atgccgagca
     2101 cggtacgctt gtacttcttc ttgaaatctc ttttggtaag ttcctcaaaa agaaatctgt
     2161 actgcttgac tttatcgatg aaagtagtat tcggcgatga gttaatagac acctttacgt
     2221 tgtcctgctt atgcgcaatt tcttttacag gctttgcggc agg
//
LOCUS       DAWAOG010000339         2261 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6208_length_2261_cov_2.164551, whole genome shotgun sequence.
ACCESSION   DAWAOG010000339 DAWAOG010000000
VERSION     DAWAOG010000339.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6208_length_2261_cov_2.164551"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1129..1138
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          545 a          520 c          553 g          633 t
ORIGIN      
        1 gagcagtatc acacgctttg ccgcagacag taacccgtca agtcggtgct ctgcaactat
       61 tatcgtagtg ccgagctcct tatttatcct tataatcaga gaaacaaact gcgcagaagc
      121 tatcgggtca agttgcgaca gcggttcgtc aaggagcagt acatccggct tcatagccat
      181 gaccgacgct aagtttatca tctgcttctg cccgcctgac agcttgtcgc agtcgcagta
      241 aagaagctcc tctatgccaa agaaaagtgc ggtctgtgct actcttgaac gaatttcact
      301 ttgcgaagct cctatgctct ctgccccgaa agcaagctca tgccatacct tgtcgcaaac
      361 gatctgctca tcgggaaact gcgatacata tcccgcaaag ctcccgtaaa aacttatttc
      421 tccgcttgtt tttcccgctt cggcgggtga tttcataagc tccaagagcg ttgactttcc
      481 gcttccggag agtccgcaaa gtacgataaa ttcgccctta tccacactca atgagatatt
      541 attcaaagca gaactgtcgc agtttggata gttatatgaa aaatctgata tattataaag
      601 tttcgtttca aaccctccgt ttcatatcgg tatagatttc agtcgctgtc ggcaaaacac
      661 atagtatgcc ccataacgaa tatgagatga tattgcccac agtaagcggc aattcaaacg
      721 ccgggtagta attataatac gcataccctc ctgcataaag caaagtgatg cctaccgccg
      781 tgataactgt aattgccgta attacaatat cggtcggggt aaagctaaat acgctgtagg
      841 cagtgcgctt tgcagtgccg taacctctgt atttcattga gcgcgccgta tatgcactgt
      901 gttcaagcgt gtccgttacc gcaaccgaca aaacatgaac accgcttctg atacgttctc
      961 tgagtgtgcc tttgtatata tcgtttccga caaagcgttg agcggcggca gtctgctttg
     1021 cacgccttgc gaacatcggc acggcacgga gtgtcattga taaaagcaga gcaagagatg
     1081 gcaggagctt gccgaaaaga tatactgttt tgtcggtcgt tatgcactnn nnnnnnnnca
     1141 cttgttaagt ccgataaacc acatcacaag agatactgcc atagctgcgg taaacaatga
     1201 aaatataagc gtttcaaggg ttacgttatt gccgcccggc agtctgaaca atggggtaac
     1261 gcccgcattt gaaaaaagca tatttatgag caccgccacg acaattacgg gagttgccgc
     1321 tataagtgtc ctgacagcaa gccgtctgtt tatcataaag gcaaacaatg cggaacagac
     1381 aagagctgtt gccataaaaa caggattttg cactattgcc gttatcagta tcaccgacag
     1441 aaaatatact gccgcagtga cagggtgtgt cctttccatt tttcctccgt attacaggtc
     1501 atagccgagt tcgcaggtgt agcggaacgc tattttatca ttgttttcgg ggatatatga
     1561 tgagcagccg tagttcggga aagctccgtt cacgctgtac atccaacctg agcttccccc
     1621 gcagtcaaac tcatacaggt tgttgatacc ctcaacataa tatgagccga atgcgggcgt
     1681 gaagctcgat tccatttgga tcttattctc atcacatatg cggcggagaa tatcgtatac
     1741 gctctctccg ctgtcaaagc ccactgttcc gccgaatatc attccatctg tcggaacgta
     1801 gtctataaga cctttcctga gcttatcctt attatcaagt attgttttgc attctataac
     1861 gaatgagcag gagttgtcat actttttgat cggctgactg tccgcgcctt ttgtggtagt
     1921 ggttcgtacc gtggttgtgg tggttttagc ggtggtggtt actttcgcag tggctttcgt
     1981 ggttgctttt gcggtggttg tgacggttgt gatcggcttt ggggtagttg cagaagtctt
     2041 tacggtcgtt gttgtagctt ttacagtcgt tgtcggcttt ggcgttgttg tgggagtttt
     2101 tacggttgta gcgggctttg ggggtgcagt aggctttggt acgggcgttg ccgaggcggt
     2161 ggatgcggtt gttgacgcag acgttatcaa ggtgttggcg gtagtcgtat cgggggaggc
     2221 ggtgccggta tctgtttgcg aaacggtttg caaatcaccc g
//
LOCUS       DAWAOG010000340         2259 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6226_length_2259_cov_2.054900, whole genome shotgun sequence.
ACCESSION   DAWAOG010000340 DAWAOG010000000
VERSION     DAWAOG010000340.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6226_length_2259_cov_2.054900"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1002..1011
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1358..1367
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          703 a          446 c          582 g          508 t
ORIGIN      
        1 gtcatatcat gcacgccctt atggactatg gctatacgac cgacctttac ggaaaggtat
       61 atgatgagct tttcaatccc gtgacaggtc tttgtgcaga agaggtgagt accgacatgc
      121 aggattatcc ggaaattcat tttgtgctgg aacttatcca ttcggcgggt ggtattgcag
      181 tacttgcgca ccctgcagta ttcgataatt ttgaactgct tgaggagctt gcctcggcag
      241 gcaaaataga cggtgcagag gtatggcatc agagtgctac ggaagaacag cgcgaaaggc
      301 tgctgaaaat agctgaggat cataatctca tcacaaccgg aggatctgat ttccacggat
      361 tttacaacca ctatcctata gctataggaa caaactacac gccggacaca tcacttcagc
      421 gtatactgaa gaagaaaata aaataatcgg caaaaggaag gaaaagaaaa atggcaaccg
      481 atatcaaaca acaggcattg gaaaagcact atgaatggca gggaaagatc gaggtcatat
      541 caagagctcc cgtcaataca agagaggatc tttctctttg ttacactccc ggtgttgcgc
      601 aggcctgtct tgaaattgaa aaagatccct cactgtcata caagctgact cgccgaaaga
      661 atttagtagc ggtcatcaca gatggtactg cagtacttgg acttggcgat ataggcggac
      721 ttgcgggtat gcccgttatg gagggtaaat gttgtctgtt caaagaattt gcaggtgttg
      781 acgcattccc tatctgcata aagtcaaagg atgtagacga gatagtaaga acgatcgagc
      841 ttatctccga cagcttcggt ggtatcaata tagaggacat ctcagcaccg agatgctttg
      901 agattgaacg cagactgaaa gaaaagctcg atattccggt atttcacgat gatcagcatg
      961 gaactgcagt tgtagttggc gcggcactta tcaatgctgt annnnnnnnn natgctgtac
     1021 gctgtgcagg caagcagatg aaggatatta ccgttgttat aaacggtgcg ggcgcggcag
     1081 gcatcgcaat tggcacgttc ctcctgaata tgggtatcgg taaccttatc atggttgata
     1141 taaacggtat catcaacaag gatgacaagt acgaaaatcc cgctcatgca gagatcgcaa
     1201 caaagacaaa caataataac ataaagggcg gacttgccga tgctatgaaa ggtgcagatg
     1261 tattcatcgg tgtatcaaag cccggtcttg taagcgagga tatggtaaaa tctatggcgg
     1321 aaaagcctat agtatttgct atggcaaatc ccgagccnnn nnnnnnnagc ctgagattat
     1381 gcccgacaag gctattgccg caggcgcata tatcgtaggc acgggcagaa gcgactttcc
     1441 taatcagata aacaacgtgc ttgtattccc cggaatattc aaaggtgctt tacagtgcgg
     1501 agcaaaggca attacagaag aaatgaagaa agccgcagct tatgcgatag cggatattgt
     1561 aactcccgac aagctgtcga gagaatacat aatccccagc ccgcttgaca agtcagttgc
     1621 caaggcagta gccacagcag ttgcaaaagc ggcacagcag taatataatc ggcacagaca
     1681 atttgaaagg aagacgaaaa atgacatttg aatacacacc gaagggagta tgctcccgta
     1741 aaatgataat tgatgttgaa aacggcaagg tagagggact tcaggtaata ggcggttgca
     1801 acggaaatct gcaaggtata gcccatcttg taaagggtat gtctgtcgat gaagttatcg
     1861 agcgtcttga cggtataaag tgcggttcaa agagcagttc atgtcccgct cagcttgccg
     1921 aagcactgaa agaatacaag gcaaacagct gaacgacagt aatgatttta cagggaggtg
     1981 aaacgagtgg cagaatactg ggacgtatac gataagaaca gaaaacgtac cggaaagctt
     2041 cataaaagag gtatccgtat gcagccgggc gaatataata ttgtctgcga ggcgtggata
     2101 gtaagcggta acaagctgct cgtaacacag cgctgtaagt ataagaattt cggtggtctg
     2161 tgggaatgca caggcggtgc ggtcaaggcc ggagagtcaa gtattgactg cataaagcgc
     2221 gagatcaagg aagagatcgg tatagaagtt accgatgaa
//
LOCUS       DAWAOG010000341         2255 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6235_length_2255_cov_1.594091, whole genome shotgun sequence.
ACCESSION   DAWAOG010000341 DAWAOG010000000
VERSION     DAWAOG010000341.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6235_length_2255_cov_1.594091"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1208..1217
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1734..1743
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          681 a          473 c          527 g          554 t
ORIGIN      
        1 cccatattcc ttgtgatgaa agaaatatcg tatggaaagc cgcaaaagcc ttctttgacg
       61 ttgcaggtgt taccgcggaa atttctgtag atatagaaaa gcacgtcccg cttatgggcg
      121 gaatgggcgg ttcttctgtt gacggtgcag gaacgcttgt cgcactgaat gaactgttcg
      181 gcaaaaagct tgatacggac gcactttgtg ctatcggcaa caaaatcgga gcagatgttc
      241 ctatttgcct gaaaggcgga acgcagtaca gcatttcaag cggcgatatg ataactgccg
      301 agtgtgcgca taactgcgta tttctctgta tttatccgga ttttacgctc agcaccgcag
      361 aagcttatac aaaatatgac gaaaacccaa ccgtgataaa tcctcattac gctgattttg
      421 taagcagtat agtctgcggc agtctttcag acggtgctaa atacataagc aatgtattca
      481 cagggatcta tcacgacaga cgcattgatg aaatcaaaaa cgatattctg tcggcaggtg
      541 cgatatcgtc cgaaatgaca ggaagcggaa gcgttgtttt cggtatattt gaaagtgcag
      601 aaaaagccgg agcggcaatg gatattctgt ccgaaaaata ccacgatgtc tttacagctt
      661 atcccgttga ttgcggcgta tcggtcgttg cacggtaatt tagcataaac gcttgaaatt
      721 taccgtgaaa tatagtataa ttattaagtc cgatttacaa ggaggtaaag ttatgatcac
      781 tataaacaac cacttaggaa cgatcaccta ttcaaaacag ttcttctaca gccttatcgg
      841 cggaactgtt accaattgtt ttggcatagt aggaatgaat gctgttgacg caaagcagac
      901 attcatcgaa aaagtacctc ttaaattcgt caagaagttt gccgacaaga ccattctttc
      961 cgaaaaaggt gttacggtaa aatataaaaa cgacaagttg tatatcgacc ttcacatttc
     1021 cgtaatgtat ggcgttaatg tgtcaacgat agtaaagagt atcattcata aggtaagatt
     1081 tgcagttgag gacgaaacag gatttaccgt agataaggta aacgtttttg tcgatgctgt
     1141 caaagtataa atcaaggaga gtatctgact gatgttaagc ggaaaaattc tcagagatgc
     1201 tatcatcnnn nnnnnnncgt tgacgaactt aacgtattcc ccgttcccga cggcgacaca
     1261 ggcactaata tgtctatgac cataagaaat gccgttgcgg agctgagtat gttaagtgac
     1321 agtgcaacag tagaaaccgt agcgcagaca gcggcttctg ccatgctcag aggtgcaaga
     1381 ggtaactccg gcgttatcct ttcactttta ttcagaggac tttctaaggg gcttgcagga
     1441 aagcatgaag caactgtaga ggactactgc aacgcactca aattaggcgc agaggcagcc
     1501 tataaatcag taatgaagcc caccgagggt actatcctca ccgttgcaag agttgccgca
     1561 gaaaaggcaa atgacgctca gtgcaaggac ttccccgagc tgtttgaagt tctgacaaca
     1621 gctgcaaagg aaactctcga tcagaccccc gaaatgctcc ccgttcttaa aaaagcaggt
     1681 gttgtagacg caggcggaat gggctattac actattctca aaggaatgtc ttcnnnnnnn
     1741 nnncgatgta agcaaggacg caacaaagct tcgtgctttt cttgaatcga taggcgactg
     1801 cgttgttgtt gttgatgatg acagtatcat aaaggtacac gttcacacag agcaccccgg
     1861 taaggcactt gaagagggca tactttacgg ctcgcttata aatctcaaga ttgagaatat
     1921 gaaagaacag cacaagggtg cggcggcaaa ggctgaaatg cagaagaagc agaagctcgc
     1981 ccccgcagag cctaccaagg atttcggctt tgtatccgtt acatcgggcg caggactcga
     2041 agagctgttc aaggatctcg gcgttgatgt tatagtacgc ggcggtcaga caatgaaccc
     2101 ctcaacagaa gacatacttg aagctatcaa cgctaccccc gcaagaaaca tttttgttct
     2161 gcctaacaac aagaacatca ttatggcggc tgagcaggct gtaaagctta ccgacagaaa
     2221 cgttatagtt ttacagacga gaacaatacc tcagg
//
LOCUS       DAWAOG010000342         2254 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6236_length_2254_cov_1.906321, whole genome shotgun sequence.
ACCESSION   DAWAOG010000342 DAWAOG010000000
VERSION     DAWAOG010000342.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6236_length_2254_cov_1.906321"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    716..725
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1110..1119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          648 a          397 c          509 g          680 t
ORIGIN      
        1 gttatatgtc acggcaggca cgagcgggga gtattatctt gccgccgccg ttgttgtagc
       61 gtcgggaatt accgattttc ttgacggaca gatcgctcgc cgttgtaata tgatcaccga
      121 gttcggcaaa ttccttgacc cgcttgccga taagctgacg cagggaacat tgttcctttg
      181 catagcgata aattatccgc ttatgtggat actgcttgct atatggcttt taaaggacgg
      241 ctttatggca attatgggac ttgtgctttt aaagacaaag cagacaaagc ttgacggcgc
      301 gcgctggtac ggaaaggtct gcaccgctat tatctatgtt gcggtactgg ctctgttgtt
      361 cttcccggga gtattccccg agggatcgcc tgcatcatcc ttacttattc ttatctgtat
      421 aggagtaatg cttctttccg gtattcttta ttcggtagca cttataaaga tgtggctgca
      481 taatgaaacg atagccaaag aggacagaaa gaagttcagg gagatagcta aggaaagtac
      541 gcacgagcat agtgatataa agagtgaata agaagatgac ctacaggtga gcctgtaggt
      601 cgtttttgtt ttcagcggtg ttggatatgt cttttatgct ctgataattt aataaaagat
      661 tacacgcttt gccaagcact gattttagct ccctttacac aagggagcta aattgnnnnn
      721 nnnnnataca acattctcaa tttcgttttc tactatgttg aagcgcttga actccggtat
      781 ggattcatca aaacttttta tcaatatgct ttcgtcgccc gaaagactgt acattgccat
      841 tattcccgag ggataaagca ctttgtcgag cttgcccgtt ctcagcttat catatccgtt
      901 ataaagcggg atatcgttct tgcgactgag gtaatcgatc atagcaagca ggtaaagaca
      961 ttcggcgtac cacttacgtt cgtacagctt gcttacttcg tcactgcaca gcatatcacg
     1021 gataaaggaa agctcgccct tgtcttttac aaggtggcag atgttgcttt tgaatatttc
     1081 aaacgagctt ctttccatgt gatttccctn nnnnnnnnnt tttagcacag tatttatgtg
     1141 atgtcaagaa agctttttcg gtcgcagaaa ttgcaggctc acgaaaatgc cgtttattat
     1201 gtcggttgcg ttgaattatg ctgtttttta tgttattata aggtagacgg ttttaatgcg
     1261 gtcaattgac gttataggtt caattaatgt cggaattttt tctgtgtact ataacaatac
     1321 aatgataacg tcaatgccga atttaaatat ttgaacataa ggaattggcg aaaaatgaca
     1381 gaaatttcgg agaaattaaa atgaacgaat taagtattaa agattatatt cgcaacgggc
     1441 ttttctttga agcgcaaagc gtggcgttgg attttgtttc atttcttgaa gaaaaaggcg
     1501 tagactttta caaggacaac tgcgattgtt ggaaagataa aatctactat cagtgtaaat
     1561 tcaacaattg ttgtgtatgc tttatttcaa tcaaagaccc cgatgagccg caagatctat
     1621 ggactgtatg gtctgatgat ataaaatcgg aatggcttga taaatgtgat gttgatgatg
     1681 aaataaaaaa gcttgcgtgg aagtatgtag ataactgcgg tcattgcggc tcatgcaaag
     1741 gcggacgtcg taaagtcatt ttcggacggg agtttgaagc tgtctgcggt tgtacattca
     1801 gggtcgataa tccgactatt gaagatctac cgtttctgaa ggaaataatc aggctgtgca
     1861 taataaactt tgaaaaggaa acaaaacagt gaatatcaga actttgaaca caggcgaatt
     1921 aaatctgctg tttcagctct ttgactacaa cgatcccgat gaaatgatag ctgaaaatac
     1981 agtagatatt aacgagagaa aaatcgatat attcggcttg ttccaagagg acagactgat
     2041 aggagagata cgcgccgctt atgtccataa cgatgaaagg catgccgtaa gaaataagcg
     2101 tgtttatctc tttgctttcc gtattcataa ggattttcaa ggcaggggat acggacaatt
     2161 tttgttgaaa gaggtaattt ccacgcttat gcaaaaaggt tattatgaat ttacgatcgg
     2221 tgtcgaagat gataacgaaa cggctgtaca cata
//
LOCUS       DAWAOG010000343         2252 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6246_length_2252_cov_2.458807, whole genome shotgun sequence.
ACCESSION   DAWAOG010000343 DAWAOG010000000
VERSION     DAWAOG010000343.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6246_length_2252_cov_2.458807"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1218..1227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          669 a          540 c          499 g          534 t
ORIGIN      
        1 ctaacttctt attgtaaaac aatgcatatg tttcgcatga catagggtat ccgtacatta
       61 catcattata tacggtagct tttcttgcaa tctccaacgc cgtggtattg actatatccg
      121 tatcctctgt cggaagtacc agcttatttt tcaccagttc acccgtcata tcaccggggg
      181 cggcaaaaac atcgggcttt ttaacggtgc tttctgtatc aagaagctcc ggtgtggatt
      241 ctttcaggtc aacattgaca tattctatgt gtatattggg ataaagtaaa ttaaaactct
      301 ctccagcttt ttttatccac tcatcgggac cgtctttgga ttcccagacg gtgagtgtga
      361 tatcatctct tgtaaggctg tcgtcggtcg catacggctt tgaactgctt acgtcgtccg
      421 ttcgtggcgt atgctgaata attatatgac tgctttcgga acagcccgct gtcacagtgc
      481 atataagcac ggcaagcata gctatcgctc ttttgtatct catatctcct ccaacggata
      541 atcacaactg tatttttact gtaataattg tacaacaact gcccgatttt gtcaacatcg
      601 gatgtacttg caaatgccgc gccgttatga tataattaca gtaattaccg ctagccggca
      661 gtacatgaca catacattgg tatgcactgc gtgaaaggaa ataaaatgaa ccctgaaaag
      721 aaatttgaaa caagcagtat agatgatatc aaagctcttt ttgaaaacag aacggaagaa
      781 gatattatcc cacccgattc gttcggcaaa agcgaaaagc aggtcgtaac aaaagaagct
      841 gtgcaaagcg cagtcagaaa gacagcatca gaaagcataa aaaagcggaa acgaaaccgc
      901 acaatagcaa atattatgat atacagcggt atcggactgc tgatagttgc ctgtgccgta
      961 ttggtagccg ttatattcaa cggtaacaac ggcagcagta taaagagtct tgacattaca
     1021 aacagcggcg aaataactct gcgcgtcgga cactcacagc agataaaggt aaagacagag
     1081 ccagccgaca ccgaatatgc cctgtcgtat gtcagcggtg acacatcggt agctactgta
     1141 tcacttgacg gaaaagtcac tgccgttaaa aacggaaaga cagagatcac cgtaagcagc
     1201 ggcgagcttt ctgacacnnn nnnnnnncaa taacggtaaa tgtcaaaaaa gatataatag
     1261 agtcgcttga cgtttcgctt gcggcgttat cactcgacgg cggcgaagaa cagaagatag
     1321 acgcaaagct tactcccgct gacgcaaaag atgttaagct gagctggaaa agtgcagata
     1381 cagatgtagc taccgttgat aagaacggcg ttgtaaaagg ggtaaatacc ggcgaaacaa
     1441 ccatcactct gaccgattcc gtaacgggtc tttcaaaaga tatcactgtc accgtgaacg
     1501 gacttgaact ccccgaatcg atgaaattcg acaaggacag cgtaactgtc gaggtaggag
     1561 atgtgtataa ttcagttctt aagttcactc cggaggatat tacctacacc ggtgccatat
     1621 actataccag cgactcatcg gtagcgtccg tcacaaacga aggcgttata accgcaaaga
     1681 gcgccggaaa ctgcaccatc gaggcgtatt acgaaaacga ctaccgcctt gtggcaacta
     1741 tggaggtgac cgttatagat cctttcccta taacaagtca ggaaacaacc gccccggaaa
     1801 ctccgtcacc tgaaacaaca actcccgata ccgagcctga gcccgagcct gaaaccgacc
     1861 cgccggcttc ttcaacgccc gctcccgcac ctgaatatag cggctcgcat aaaatggaag
     1921 tgatagacgg catcacatat tttgacggtg ttatgatagc aaataaaacc tacacccttc
     1981 ccgcaagcta taatccgggc gttcagcccg aggcgatgga cgcattctac gatatgcaag
     2041 cagctgcggc ggcagacggc atttcactct ggatactttc gagcttccgt tcgtacgagg
     2101 atcaggatgt tatctataac cgttatgtgg cgcaagacgg aagagatgcc gcagatacat
     2161 attcgtcacg cccgggtcat tccgatcacc agacaggata tacgttcgac cttaactctc
     2221 tcgaacagga cttccagtac gaccccgcag ga
//
LOCUS       DAWAOG010000344         2250 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6252_length_2250_cov_2.005011, whole genome shotgun sequence.
ACCESSION   DAWAOG010000344 DAWAOG010000000
VERSION     DAWAOG010000344.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6252_length_2250_cov_2.005011"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          513 a          581 c          475 g          681 t
ORIGIN      
        1 aatgcgtctg cttcgttgtc tgcatattcc tgagcctgct ttgcattgtc ttccgaaaca
       61 ccgtttaaga atacgactgc agcttcggga gttgtaggag cgataaccgc attttcccat
      121 gcaaatgtga agtcactgcc tgccgctgtg cttgcgagtg ctacataagc actgccgtaa
      181 gccttgcctg ttacaactgc gatctttgct gttgtagctg atgcataagc ctgtgcaagc
      241 tttgcactgt cacggatgct tcctgcaagc tctgccgcac ttgacttttc aaagccttcg
      301 ctgtcaacga atgtaacaac agggatcgaa taagcgtcgc aaagtgtaac gaaacgagcg
      361 atctttgcgc tgtcctgagc tgtaagcttt gtaccgttgt ttgtaccaac aaagcctacc
      421 gttctccagt taaggcttgc aagagctgta acggcagatg taccgaactg cttgtaaagc
      481 tcgatcacac tttccttatc ggcaacagcg tttataaggt ctgtaccctt gaggtcagcc
      541 gaaactgttg catcgttttc catataataa tcgtttcctg ctgtttcaag attgttcgca
      601 gggagaacgg ataataactg cttagcctta gctatagctt catcgctgtc ctttgcaagg
      661 atagcggcta caccggactt agcggcattc tcggctgtac ctgcacctgc cgtcttatca
      721 tcggctgtga aaggtgctgt catgaacagc tctgattttt ctgtcataat aactacatca
      781 gccatacaag ctatcatagc ggctgtgcct gcgcaaagac cgtctactac agctatctgt
      841 ggaacagcac ccgagagtgc cgcactcatc ttagctatct cgccgtatgc accgagtgtc
      901 ttcatacctt ccttgatatc tccgcccttt gagtcaaaga taccgataac aggagcgccg
      961 ttcttaagtg ccagctcgta aaccttcttt actttgagag ccgcagcagt gtttacagca
     1021 cctgcattaa cgcttgtgtc ctgcgcgtaa gcatatacga ctgctccgtc aacatagccg
     1081 taaccgctta caacgcctgt cttttcaccg tttgctccga caaatttgcc aagctcgaca
     1141 aaactgtcct cgtcaaacag agagatgagc ttgtttgcag cttcgcttgc ggctgtctgc
     1201 tccatttcag caattgtttt cttcattgcc agaattcctc cgttctcgct tttctgtgaa
     1261 gcggtaagct gctgaaacaa gagctgaccg ccgttgtata attatgccag tggcacatca
     1321 tattcaagta taattataac tcattttagc tttaataaca agcgaaattc gctatttttt
     1381 gcaactttta tgagatgtga aaaaattatc aatgtctgag atgtgattat cgttgttttg
     1441 cacaaaacca cgcttctgaa gctcattcca taccgtttcc gactgcatat ttacggtgca
     1501 cttatttata tagtgattga gcgcgtagtt catagccgtc ctcataagtc cgtcaacaag
     1561 cattatgtac gatgtttcta gcttttccac aaacagtgtt tcgccatcag ttgagcaggt
     1621 gacagagcct atgggtgtat catcgtcctt tgcctcaaat acgagcttgt catttatatc
     1681 cttgtttctc agtatagtga tcattttctg cctgtcctct ttgccgactt gtcgcttgaa
     1741 acaagagcct tgagcccctt taattcctcg gcggtaagct ctgcccattc gccgggcttc
     1801 agcataccga gctttacatt gcctacagca tttctcttaa gacgcactat ctgtaatttt
     1861 acagcctcgc acatctttct tatctgtctg ttcttgccct ctgcgatcgt aaattcaagc
     1921 acggtcctgt tttcttcttc ggtcagaacc gcaacagtgc agggaagcgt aacccccgtg
     1981 tcaatcttga cacccgatgc aagagtagtc agcttgtcgt ctgttacctc gcctctaacg
     2041 gttacacggt agcgcttctt aatgtgacgt gacgggtgca ttatggcgtt tgcaagtgcg
     2101 ccgtcattcg tgagtatcag cagtccctca gagtccttgt caagtctgcc gacagggtaa
     2161 acacgctcgt ctatatcgtc aagcaggtct gttactattt ttctgccgtg tgtatccgcc
     2221 attgtggtca cataacctct cggcttatac
//
LOCUS       DAWAOG010000345         2248 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6267_length_2248_cov_2.410397, whole genome shotgun sequence.
ACCESSION   DAWAOG010000345 DAWAOG010000000
VERSION     DAWAOG010000345.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6267_length_2248_cov_2.410397"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          767 a          420 c          495 g          566 t
ORIGIN      
        1 gatattgaca gctttgtagg ctatgtcaga gcggcgtcga tcaccgatga aaaggtaacg
       61 gaaaagctcc tgccgtttga taaggctata agcaaaatag aacagtcgct gaccgaacag
      121 gtatcgtttg aggtactgag cgccgacctt gtgtatgaac agatgacata ttacggagcg
      181 tatgacattc cgcttgagga gcagaaacgg gaaacctcgc ctgcgtggaa ggtcgttatt
      241 cagaacaaga acaatgatct cgtgtacacc tgttatatag acgccgacac gggagataat
      301 ttcagttata gcaaggcggc gttctatacg gaaacggaag aggcagagga ctgatatgtt
      361 tacggcaaca ctttcttacc ttaaacgcat accgggagat acggatttac aatgtcttta
      421 ttttccctta ctcagcagtg tcaagaaaga atttgaaact tttgaagatg tactgacaaa
      481 gacaaaaaat catttacagc aggtcgacag cgatcttgaa gcacttgtcg gagtaaggac
      541 aagggcgata aacaaacagc ttatgagtgt gggtgaggat aatgcggaac aaatagaaaa
      601 ttgagaaaaa gaaacaccgt cgcagaaaat attaaattct gtgacggtgc aatattttgt
      661 aatatgtgac aaaaacacaa agaaaagttg tgaaatcata caaataatca aaaatgctat
      721 tgaaaagcgt atcagtattt gataatatat agtcaaagct attgcaataa ctttaataaa
      781 atcattacta aaaaaggagc attatcatga agaaaatcaa aaaagcatta agcattgcag
      841 tgacatcaat agtagcagtt gcaggtatct tatctgttcc ggcatctgcc gctgacggcg
      901 tatatcccaa aaggttggca tggaaagctg ccggttattg tgccccgggc ggctcgccga
      961 gcaacaccta tactcccgct acgcctaaaa tacagtactc aacatatggt cattatatat
     1021 atgtaaacgg gattgttaat tcatcagacg gcgcaaaata tgaaattttg atttcgtgtg
     1081 atagcggaac aatgacggag atatccacaa aaagtaacgg cagatatttt tgtgatccta
     1141 ctatgcatgg ttctttcgaa tgtgtgacct tcaggatcaa agcactttca tatacgaacg
     1201 gcaatacggt cacagctaac ggcagtgcgg tcacaaaaac aaaactataa tataaaaagg
     1261 agatacagta tatgaaacgc tttttaacaa taatacttgc aacattcatg ctttgcgcct
     1321 gcacaggctg cagtggcggc ggtatcaaca gcaatatcga aagtgcaagc aacagcaccg
     1381 acaatactga cggtgatatt ccggaagcat tgaaacgtaa aaacgaaata agctattata
     1441 cgagtgacga ggtaaagaag ataattaaag aaaacacgaa attcgatata tccgaggatt
     1501 tcgagagctt tgttccgaag agcgtttcat atctcagcta ttttgactat gcacctgtca
     1561 acagaccgaa tatctccgat ttttataccg acttcaagga aatgttcgca tatcttttcc
     1621 cggacgaaaa atttaacgat gactactttt tctattacta catcagaaca gatgaggaaa
     1681 cagaaaatgc gaaagaggac gaatacccta taaaaaacgt caaaaagtat tttgacgaag
     1741 cggcagacgg caagagaaaa atatcatttt atttctattc tccttattct cattttgctg
     1801 aaaaggaagg cacggattat gctcatcggg tcgaaaagga tagttcggat attaacgatc
     1861 gcaatatttt ccttgaattc ggtccgccta taggcaacta cctgtcaaat gtcaacaagg
     1921 gtgtacttgc ggaatattac agcaaggaga aaggtgaaaa gaacgattat tggcttgagg
     1981 tcacaggaac cgttatctgt gaagaatacg aaaccgtcag atcatgcact cccgattgca
     2041 cagataagta cagattgctt gacggcaaag agataagtgt tgccgatgcg gtgacattct
     2101 ttgaaaacta tatgaatgat ctgccgtgcc cgaaagatcc ggtatacgat accaaggtaa
     2161 caggtgttaa tgtgaagaag ctcggcgagg ataaatattt cttcatgttt cagataacgc
     2221 ctacctatgc gggagttccg cttgactg
//
LOCUS       DAWAOG010000346         2234 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6317_length_2234_cov_2.491051, whole genome shotgun sequence.
ACCESSION   DAWAOG010000346 DAWAOG010000000
VERSION     DAWAOG010000346.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6317_length_2234_cov_2.491051"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1430..1439
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          554 a          476 c          480 g          714 t
ORIGIN      
        1 gtgcagttgc accgatatac tcacgaaatt attaacacct tatcggtacg gaaattgttt
       61 tcagcgttgt tgccatttgt ttaattatac cgataaatat taaatgagca ctgcctttta
      121 ttctttgatt tttaaattaa cagggcttct gaggtgaaca tcgcatataa gctttatttt
      181 tttattatct tttctgcggc ggaggcaagg tatagcgagc ctgttatcat tgctatatcc
      241 tttgtacccg ctttgagaag cgcctgtgat atagcgctgt caaggtcggt gcacgatgta
      301 cagcgtactc cctgctctat ggcaaagcgt gtaagctgag aagcgggaac acagcccggc
      361 ataaagccgt caacaaaaat aacgtcttca aggcgcggaa gaattatttt aagtgcggtc
      421 tgccagtcct tgtttgtcat cattccgatc acggcgtgta tcctgcggtg gtcgagctcc
      481 agcagcttta ccgccgcttt cattgcagag gggttgtgtg aaccgtctat aatgcatgat
      541 gtaccgttta ccgtaaccga ctccattctg gctctgaggg cggatctttc aagtcctgtg
      601 aatacactgc tgtactcgat tttcagtata cggcaagcct caattgcgca gagcgcattc
      661 tttatctggt gctcgcctga catcacaagg tgataattca tacctttata tgagaagaat
      721 acatcggtcg tgtccatatg gagtatctgt actcttgata tatcgggaat tatctcttcg
      781 cagttatact cgcggcattt atcgtcaatt acacgcatta cctctttttc ctgtccggga
      841 gctaccacgc acggaatgga cggttttata atgcccgcct tgtgacgggc aatatcggta
      901 agtgtttctc cgagaatgtc tgtatggtca taatctaccg ttgtgatcac gctgacaaca
      961 ggatttacta tattggtgca gtccagaagt ccgccgatgc ctgtttcgat aacggcgacc
     1021 gacacttgtt ccttatagaa ataaagcaga gcgatagcgc acagtatctc aaactgtgaa
     1081 aagccgctaa atcctgtttg ttcgaccgcg ttttttacct gctcacacag cagtgcaaac
     1141 gcgggtcggg aaatatattt atcgttcacc tgaatgcgct cttctatgca gtcaatgtag
     1201 ggagaggtga actttcctac gcttttgttg tccgctttta cagcgttgct tatatattcg
     1261 cagacgcttc cctttccgtt tgttccggca acgtggacta tttttaattc gttgtggggg
     1321 ttgccgagaa gtcgcataag gataacgaac cttatcagat cctttacagg ttcgccagat
     1381 ttggcgaaag aactgacgta ttcaaatgct tgttcgtatg tcatatggcn nnnnnnnnng
     1441 ctttcctttc gtattttctt cactcaaaac acaatcactt cataagtgca ataaagctct
     1501 tcgctttatt aactatcaat ttatcattat catagacaag cagattatgt gcttgtccta
     1561 ttccgaccca gcggatatcc gaaatttctt caagctgagg ggtataatca taatttcttg
     1621 cacgggcgac aaaatataca acatcttttt ttgagtcctt gcggggcgaa tatgtaaccg
     1681 tttctctgaa gcccgaatca atataaacgt ctattccggt ttcttctttt atttcacgct
     1741 ttgcggtttc ctcttctgtt tcgccgtttt caacgtgccc tttcggaaaa gaccaatagc
     1801 cggatttgac gtgttttatc agcaatattt cggtattacc gtgaaatttt ctgtaaacta
     1861 ttcctccgca ggacttttcg tgagtcatct gctacgaaca cctccttgtg tctgccctct
     1921 gtctgtgcgg caaagttgtt tataatcgtt tatattatag catatttttc actaaaagtc
     1981 tatagcgggg gagggattgt aaataatttg ggagataaaa acaaatagtg attttaggga
     2041 attgcggcga taagcggata gacgtacttg ctgttatctt tgttttgtgt tatcgaaagg
     2101 ttttaaacag gaaagtggct gtgacacaac ccgaaaaagg aaaagtgagt cgaagcgcaa
     2161 aaatagtgcg ctcggctcgc ttttttgtat ggtttgctcc tattatattg ctttagtaat
     2221 taacaatgat agca
//
LOCUS       DAWAOG010000347         2229 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6342_length_2229_cov_1.937902, whole genome shotgun sequence.
ACCESSION   DAWAOG010000347 DAWAOG010000000
VERSION     DAWAOG010000347.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6342_length_2229_cov_1.937902"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    522..652
                     /estimated_length=131
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          641 a          430 c          521 g          506 t
ORIGIN      
        1 gagcagtaca gatacgtctt cggcacggca ttcttcgccg agcgtttcgc ccatcgactg
       61 cataagctca cggtcaaagc tgcaagcggt agcgcaggcg gccgggaagc agacagcctc
      121 tatactctga tttgggtcaa ctgtgtcaat atcctgtttg cgaagcccgt gaggaccgtc
      181 gctcaccata acatcgggga tacccagacg atcaatcttt ttcgtgtgcc agaaatccgc
      241 acccgagcaa agacctgcct tttcttcaag cgtcatttct gaaataagtt tatcgatatc
      301 gtgcatttta ttattccttt ctgtgtgaaa tttggttatt ttgatgactc aattataaca
      361 tttgtgcccg aaaatgtcta tagatttttt ccgaaatttt catataaatt ctacataggt
      421 ataacgtcga tttcacacgg caataatata atcaggctat taaaaccgta aaaggagggt
      481 ttcaatgaac agaaaaatga agaagtacag gtatatcata tnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncacccgga
      661 ctttcgactt catctcagga aagcgccgga agctcatcgg aaaatgtaag cagtccggaa
      721 aacaacgata aaaacagcac actgtccgca cagggtctga gggacgctat agcaaatgct
      781 tacggcgaca actatctgcc cgatcaggct atggacgcag aaatgcttga aagcgagttc
      841 ggacttaaaa aggatatgta tgatgaggtc acggcagaag cgccgttaat aggtttccac
      901 cccgacaggc ttgtaatagt caagccgaaa aagggtaagg aagccgatgt aaagcgtgca
      961 cttgaggacg cactgcttgt tatgaaggaa cagcagatgc agtatcccgt aaatgttgca
     1021 aaggtaaacg ccggcaagat actcgaaaag gacggttatt actgctttat gatacttggt
     1081 caaacagacg atacaagcga aaatgacgat gatgcggcaa agtttgcgga aaagcagatt
     1141 gacatcggcg ttaaagcgtt tgataattat tttgcgtaaa cgtgtaagcg atttttttga
     1201 aaattagtgt tgacaaatcg caaaaacagt gctatactct aaatcgataa agaaaatgca
     1261 ttgacgagga agtcggcggg aaaagcctca cagagagctg ttggaggtgc gaaacggcag
     1321 gtaataccgc ataagataat cacctctgag cagacaggca gaaacagcag taagcctgta
     1381 cgggcactcc cgttacagag tagcatattg acggatatgt acagagcggc tcgttatttg
     1441 cgggcaataa gagtggtaac acggagactt taagccttcg cctcttgaaa catcaggttt
     1501 cataaggcgg ggctttttta ttttataagc taccggaaag tgctttgtat cattgaaatc
     1561 gtaaaatcac ttataaagca aagacaggaa aggaaagaag aaagatgttg acattagaca
     1621 aggtttacca tgcggctttt aaactgaaag aggtgataag acctaccgat cttatccacg
     1681 ctccccagat aaatcccgat gcggacgttt atctcaagac ggaaaattta caggtaacag
     1741 gctcattcaa ggtaagaggt gcatactaca agatctctac ccttacagat gaggaaaaga
     1801 aacacggagt tatcgcgtgc agtgcgggaa atcacgcaca gggcgttgct cttgcggcac
     1861 agagaagcgg tatcaagtcg ataatatgtc tgccggacgg cgcacctatc tcaaaggtgg
     1921 aggctacaag aagttacggt gcggagatat gtcttgttga gggcgtatat gacgatgcgt
     1981 acaacaaggc tttacagctc cgtgacgagc acggctatac atttatccac ccgttcaatg
     2041 atgaagatgt tattgcagga cagggaacga taggtcttga gctgctggaa cagcttgatg
     2101 atatagacgc tgttgtagtg cctatcggcg gcggcggact tatctcgggt gttgctttca
     2161 caatgaagtc attaaagccc gatataaagg tatacggtgt tcaggcggca ggcgcaccca
     2221 gtatgcaga
//
LOCUS       DAWAOG010000348         2222 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6366_length_2222_cov_2.304569, whole genome shotgun sequence.
ACCESSION   DAWAOG010000348 DAWAOG010000000
VERSION     DAWAOG010000348.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6366_length_2222_cov_2.304569"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          584 a          490 c          545 g          603 t
ORIGIN      
        1 gccgaaagcc ttagcgcccg tttcggtagc gattttcagc gcctgacgtg ccgaaactgc
       61 tgtagcactg cggcagttgc ccttgtgagc aaggcagata aagttcatat cgctgaacat
      121 attcagcgta ttgttgcttc ctgcgctgtc agtgccgata cacagcttta cacccttgtc
      181 gagcatatcg ggtattcttg caaaaccgtt tccgagcttt gcattgctga taggattagt
      241 agcggcatat acgcctcttt cagcgagaat atcaatatcc tcgtcggata gctgtacgca
      301 gtgagcggca accgtatgga acttgaacat ttccatatca tcaagatact tgacaggcga
      361 acagccgttc tgcgctatgc agtcctcaat ttcctttacc gactccgaca ggtgaatgtt
      421 tatgccgagg ccgtattctg cggctttctc cattacaagc tcaagatagc ccctgtcggt
      481 agtatagatt gcgtgaggac ccatcatgaa tttaagacgc ttgttgtcgc cataggtgcg
      541 catatcgtac agcgtgtcct caattcttgt ctttccgccc tcatcatctc tgccgtttcc
      601 tacaagacct cttgatattg tcgctttcag accgctttcg cttatcgctc ttgtaatgca
      661 gtcacgggca atattcatat cgagaaaagc cgttgtacct gtctttatca tctcaaggca
      721 ggcaagctgt gtgccgtagt acatatcctc ggcggtcagc ttgtcctcca tcggcattat
      781 cctgtcgaac agccaggtgt ggaacataag gtcgtccgca ctgtttctga acagcgacat
      841 atagctgtgc gtgtggctgt ttacaagtcc tgctgtaatg agcttgtccc tgccgtcaat
      901 cgtcctgtct ggaacaaagc cgttttttac tgcatcgtca ccgatatagg agataacgct
      961 gtcgcctacc gcaatatcgc atacctttgc ggaaaatccg ctttcttcgt ccggaagaat
     1021 tgcggttatc cctttaaata atgtattcat ataaagtcct ttcgtccgtt tttttatgga
     1081 taattaatta tagcatttat gaaggaggat tgcaaccggg gcagggttgc tttacagcgg
     1141 aatttatgtt aaaatactac aaaaggcgag gtgataccat gaacaaagac tacacgatcc
     1201 gtgatctgta cgagatttac tgtgaagcga tgccccgcta tccgctggca tatgagcgtt
     1261 tcagggatat tctgcacaaa gataactcac agatattcac tgcggaaagc agcgaaacga
     1321 ttatcggttt tgtcgtagtg aacggtaatt ccgtagttat cctgtgcgtg aaaaaagagt
     1381 acagacacag cagttacgga aaacatctgc ttataatggc agagggattt atttccgcaa
     1441 attacgatga agttgttctc ggcgggggcg gattattcca aggtgtgcct tgtgacgagg
     1501 gcgaagagag cagtgttccg ttctttgaaa aaagaggtta caatgcagac tttacatcag
     1561 tcaatatggg acttaccctg aatgaaaaat cagtgcagaa gctgaaagaa attccccgcc
     1621 ccgacggagt tacataccgc tttgcagatg ataacgacag agaaaagctc ttagccgccg
     1681 tcaaggacgc tcaggcagat tggctaccga tattcgagga gaacaaggac gctcccgttc
     1741 tgcttgccgt ctgcggtgac gagatcgccg gctttgagat ggtcgaggag tacggcggaa
     1801 tgttcttttc cgacaatgaa aagcacggta tgataggctg tgtcggcgtt actcataaat
     1861 accgcagacg aggaatagga cttgctatga cagccgaggg cagcttgtca ctgtatgaaa
     1921 ggaattgcaa ggatattcag cttttatatg tcgagcgtgt agcgtggtac aataagctcg
     1981 gttatgaggt aacaagcaga cagtggatgg gcagaaagaa aatacaggat attatgttct
     2041 gaaataggaa cagccctgct ataacggcag ggctgaaacg ctatacgttt tcggttttta
     2101 aaagtggttg aaaaaagcgg aaatgcgcgg tataatgaac ttataagggc ggatgatctg
     2161 cccgacaaat cggaatttat atataaagga gaatattgat gaaactgttt ttatgttcgc
     2221 ac
//
LOCUS       DAWAOG010000349         2219 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6383_length_2219_cov_2.194085, whole genome shotgun sequence.
ACCESSION   DAWAOG010000349 DAWAOG010000000
VERSION     DAWAOG010000349.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6383_length_2219_cov_2.194085"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    453..462
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1421..1430
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          530 a          545 c          480 g          644 t
ORIGIN      
        1 caacgcagga gcaataatac gctctgcggc ggcgcttggc atagacgctg ttttgataac
       61 cccgtcctgc tgtgatcctc tttgcaggag agccataaga gtcagcatgg gaacggtgtt
      121 tcttatcccg tggggatata tcggagagga cgagcactgg tggcagtcgc acggccccgc
      181 atatctgggt tctctgggct ttaaaacagc ggcaatggcg cttaccgata actcggtaag
      241 tattgatgat aagacgcttc agagcgagga acggcttgct ataatactcg gctctgaggg
      301 ctacggtctg tcgcaaaaaa ctatagacgg ctgtgattat acggtaaaga taccgatgta
      361 tcacggtgtc gattcgctca atgtcggtgc ggcggccgct gtcgcattca gagagctgag
      421 agtaagggaa taataacagg tctgaattgc gannnnnnnn nngtttatta ttacattatg
      481 aatattaagt tatcttataa aattcgttct taccgccgcg tttgtatcgg gcagtgcgat
      541 accgttcttt ctgccgagct caaagcactt catcatccat gccatgttct ttgcaaggtt
      601 ctgcattgtc tgcataccct caagatcctg ttcggcttca ccgggtactc tgccgtatac
      661 catgttccag taggtagagc cgacaacagg catctgtgct atgccgaaat acttgttcat
      721 acagtcaaac gatgcagagc ttccgcctcg tcttgcgacc gcgattgatg cgccgggctt
      781 gaatttaaaa gccgcgccgc ccgcatagaa cgctctgtca aggaacgaca ggatacgtcc
      841 gctcgggtga gcataataca ccggcgtgcc gaacacaaat ccgtccgctt ctttcgcttt
      901 tgctataaat tcgtttacat cgtcatcggt gaaagcacag cctttttcac tgcactgtcc
      961 gcagccgatg caatctctga caggctttcc gcccatctgg aatatctcat atgaaatgcc
     1021 ctcgttttca agcgttctgc cgacttcaag cagtgcggca ttggtattgc cgttagcctt
     1081 ggggcttccg ttgagcatca atactttcat taaaatcgct ccttcaaata aatatgcata
     1141 aatttgcgtt acagattata tcaccttgaa taaaaatagt caagtcaaaa tttaccgcat
     1201 aacttcacgc tgtttgtcat tccgtcattc ccacaacctg cttatacatt tcttcatcgt
     1261 cataaataag ataaaagtcg gtcggaacaa aatatttttc ctgcaatgct gagctcagtt
     1321 catccgcaaa attctccgtg ctttcatact tacgggtaat gaccttttcg ctgtctgtcg
     1381 gataaatttt aagtgcggta tcgttaaaga tcatctgcca nnnnnnnnnn acaaactcgc
     1441 tgtcgttggt acggtttact atatagaata tctccgctaa gtgctccttt tgcggagtga
     1501 tcgaacctgt aaacagctcg gcgctataca ggttatcacg cagcttttcg catctgtcca
     1561 tattattctt cctccgggta aattatgccg tgtttctgcc tgaaccttgt cattatctcc
     1621 ataacatcgg cggtgtagtc aaggcacatt acgtttttgt cgccgtttac cgccttttcc
     1681 atatcggtta tttcatagta aagcgcatcg gaattttcac ctgcgccgac aagctccgtc
     1741 ctgccgtctg tataggttat ttttgcttcc cacgctctcg gatattccat gatttcaata
     1801 tagccgtttt cgcaggagat catcgcacac ttgggctgtt ttgaatggag cgacagcata
     1861 accgttgcca tctgtccctc cttatccgac agtaaaaggc tcgcctgttc gtcaactccc
     1921 gtcggtgcgg ttttaagctg tgacagcata ttatccggct ttgaatcgaa aaaccaacgg
     1981 ataacagaca gggcatacac gcctatatca agcattgcac cgcctgcaag gcttctgttg
     2041 aaaaaacggt ttttcatatc gtattccttg aaactgccga aattcattgt gataagcttc
     2101 accctgccga gtgtgccgtt tttcaggatt tcgtcaagct ttttatatat cggcatatga
     2161 taaaccgtca ttgcttcacc gattacaacg ttgttttcat cggcaagtgt gataagctc
//
LOCUS       DAWAOG010000350         2210 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6423_length_2210_cov_2.021346, whole genome shotgun sequence.
ACCESSION   DAWAOG010000350 DAWAOG010000000
VERSION     DAWAOG010000350.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6423_length_2210_cov_2.021346"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          558 a          646 c          487 g          519 t
ORIGIN      
        1 ctccctcgcg gaaaggtcgt aataccccgc gatggcgtcc accgcagcat ttgcacagaa
       61 catggtgcgc tcgtcgccga gttccttttc gtacctgctt atgtactgcc gcagatcctc
      121 cgtcctgtcc gaacctgcaa gccccgatat caccctgatg tgctgacgga aatcatgccg
      181 ctgctgcctg cttcgctcca tatagccccg aagttgctcg tatcttcgcg attcgatcgc
      241 aagcaggcgg ttttcgcgct caaggctaat attccgcaca aactcccgcg caatgcggta
      301 aaacaggaat atcgaaaaga aaaaagcgcc gaacgatatc cccgcaagca ccacagatat
      361 tctgaacacg cggtttacaa gtatcgtcgc ggggtcgctg ggaactatca tgatatacag
      421 tagcgtgaac cccatgggta tcacccacac cacacgccat atatgctcgt agcagaattt
      481 ctcaataagc caccttaccc atctcaccgc gaccgcgcag tacaccgcgc acaccgccgc
      541 cccgcataca aggcatataa gcgaggtaga tgcaaggcac accgtttcgg gatcgtccac
      601 ctcggcggca gcgttcagta tcaccgacaa cattacgcac accgacataa gcagcgacgc
      661 ggtgaaaaat acgaacgccg tctttccgag tgatatcctg tccttcagcc gcaaataata
      721 cagcacaaaa aacagcaccg cggccggaat aagcaaaagg ttgctgtcaa gtgaaaatac
      781 cgtgcatatc agcgccgaag cggcggacac cgcgcttatc gccgcaaacg ccgaaactgc
      841 cgcagtaaac ggcttttcaa gcatacgaat tatcggcgcg aagcacatcg cagcggcagg
      901 atacagcgac gcgaaatcca aaaaataccg aataaaaatc tcgatccgca tatcagacct
      961 cgtttcgcat ggttctgaac tgatactgcg taaactctcc gaaaagcttc gtgcggtcgc
     1021 gctgccttac ggggaacacc gcgccgtcgg acatttctat agcgtcgctg tcgaagcgaa
     1081 gcaccctgtc catgtttacg ataactcccc tattacacaa aagaaaccgc gggtctgcgc
     1141 acaactcgtc ctttatgccc gaaaacgtcg cggcactgcg gtacaccgtg ttttccaccg
     1201 cgatatttac cgtgtggttc tgcgcggtga cgtaggatat ttttgaaaac ggcactctga
     1261 atccctgccg cgataccatt attttaagct ccggctcacg cccgcccgcc tttttcacaa
     1321 gatcgctgaa aagcctgcgt atcctctcac cgtcgtagga tttcaacaga taatcaaacg
     1381 gatgcaccgc gaatgaatcc cacacatact cctccgatga cgttacgaac actatcggta
     1441 tgttgctgtg agtgcttctc agcttttcag cggtggctat gccgtccgtg ccgtccatgc
     1501 agatatcaag aaaaaatgcg tcgcagctta catcagcggc aagcaggctg tcgccgttcg
     1561 caaagcagac gcactctatc ccgcacacgc cctcagcggc gttttctatg gcggatatca
     1621 gccgcttcat atcgctctcg gaatcatccg caacggctat tcttaatttc atactctgcc
     1681 tcccaacttt acttttatag gaagtatatc actatttctg tcgatttgtc aagatatgtg
     1741 aattgcaacg cgtgccgata tcgcaaaaag aactcccgtg cattttcttt aacggaaaaa
     1801 tatttccgca caccaaaaag tgagcggaaa tacatattat cttcggtttg ccctgaacag
     1861 agccgcaaaa atcagccctg ccgctgttac tcctgtgctc acaagcaggc ttatcgagtc
     1921 cgaggattca ttgccatggt tacccggggc ttttcgtctg ttaaattcag cttgaacaag
     1981 ttcccattca tccggttcaa taatggcaag gtgactaccc tcggcataat actgcggaac
     2041 ttcgccctca ttgggttttg tcattttctc cgggaaatct accgtaaatg acttttggag
     2101 caatgcgtcg cccttgtatt tttcgtttgt cagaataccg gtaactgtgg ttttgctcca
     2161 ctttgcctta ccgctaggtg acgggatact caaaccctca agatatctgc
//
LOCUS       DAWAOG010000351         2207 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6434_length_2207_cov_2.403810, whole genome shotgun sequence.
ACCESSION   DAWAOG010000351 DAWAOG010000000
VERSION     DAWAOG010000351.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6434_length_2207_cov_2.403810"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          663 a          458 c          570 g          516 t
ORIGIN      
        1 gtatcacatt gtcgaagaaa agcgcccggg tgattactat gagctgtgcg agggcgaggg
       61 cggctcatat atccttaacg ccaaggatat gtgtatgata gaacatctca aagagcttaa
      121 ggacgcagga gttaccagct tcaagataga gggcagagca aaatccgcat attatacggc
      181 aacagttaca aatgcctaca gagcggcact tgacgcagtt aaaaataatg agcctgttcc
      241 gcagtgggca atagatgaag tgtataaggt gagccacagg cagtattgta ccggcttcta
      301 tttcaataaa gacgatgcaa accagtacta tgagaacagc ggatatatcc gcgaatgcga
      361 ctttgtaggc gtggttgaca gctgtgaaaa cggtagggtg aatatcacac agcgaaacta
      421 ctttaccgtc cacgatgagc ttgaagtgct gtcgcccgga aaaagacccg taaagcttga
      481 cattccgtat cttataaatt caaaagggga aaagaccgag atagcaaatc gtgcgaccga
      541 gaagatgtac tttgattatc ccgtcaattt cccgccacac tcaataatca gaaagcctgt
      601 agcgaataag taaaattacg gaggtattgt tatgctgaaa gctccgcaaa atctgtttgc
      661 gaccgcttat gacggttctg tacgatctga gtggcaggct gtagacggtg cagacggtta
      721 cattttacag ttctataatg ccgatgagcc tgaaaagtgc ataaagtcgc gttacgcaca
      781 acatctttct aagcagatat tggggtttcg taacggaaaa aaatatcttg taagagtgag
      841 agcattcaga tattcgggcg gcagggagat agcgggtgag ctgtcacagc ctgcagaatt
      901 tacgccgata tgccgacatc tcaaagcgca gaacgtgata gcaatgaata agggcgaaac
      961 gtcacagatc gtgtgggaac gcaaaaatat cgttcctgca gtcgttttca gctgtgatga
     1021 tgaaagcgtt gccacggtga caaagggcgg acaggtgact gccatatcgg acggaattgc
     1081 ccgcataacg ttgactgccg atgataaaga aacatttacg gtgaatgtag ctgtcggcag
     1141 aagtctgtca cgaagcagca gtgatgcaag gctcatgctt tgcggcgata ttatgtgttc
     1201 gctagagcaa cagagaaaag ccgcgacccg ttcgctcgac tttaccgata cattcaaagc
     1261 gcttaaaagc atgatcgggt caaccgatta ttcggtagct gtgcttgaaa caacttgttt
     1321 tgacggtgcg ccttttgaat atgagaagat aagaacggat agcggaagtc caaactgcaa
     1381 ctcgccctca acatttgtag acgcagtgaa aagctgtggt ttttccgcgc ttgtaaccgc
     1441 caataaccat aactgcgaca caggtcttaa aggacttgaa gctaccgttc ggtgcataaa
     1501 aaacagcggt atggcaaata tcgggacgct tgacgatgaa acgcatattg cggatataag
     1561 cggtataagg gtcggatttg tcgcggtaaa ctccatttca aacggacttg agaaagacat
     1621 tccgccgcac cttatcggta aatatgagcc tgtacatttc agagagcttg taaattcact
     1681 taaaaatgac ggtgcggaat atataattgc atatcagcat tggggtcaga tgaactcggt
     1741 aactgtacgc gatgcgcaga gaaaagcggc tgaatatatg gcacagtgtg gagtaaatct
     1801 tattataggt tcgcaccccc atgttatgca acgtgccgaa aagataacta ccgctgacgg
     1861 cagagaggtg atgtgcttct actcactcgg taatttcatt tcgtctatga aagaaatgcg
     1921 tgagaaccgc gagagcgcta tcgtcgatct cacgcttaca agaacagacg gctcagttac
     1981 agccgacata acctgtatac ctgtgctgtg cgaggataca tcgggcggtt ataaagtgac
     2041 tgtgcttgac ggcagtctta ccgaaagccg tagagcggca agagaacata taaaaagcat
     2101 tctgggcaac agtgcggata tcggcggtaa gccgaagccg ttattgcaag gctctgcagt
     2161 actgagaaag gtgttttccg acagcggtta taattatgac gacacac
//
LOCUS       DAWAOG010000352         2207 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6435_length_2207_cov_2.352230, whole genome shotgun sequence.
ACCESSION   DAWAOG010000352 DAWAOG010000000
VERSION     DAWAOG010000352.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6435_length_2207_cov_2.352230"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          485 a          572 c          470 g          680 t
ORIGIN      
        1 ctctcttatc atatttctga tagcgttgtt gcacttcatg atctcgaatg cggggcggag
       61 tatgccgtcc tcacaaggga taagctgctg agatatcacc gagcataaaa gctgtgacag
      121 ttggatacgc acctgctgtt gctggctcgg cgggaatatg tctatgatac ggtcgactgt
      181 gttctgcgct ccgacagagt gcagtgtcga tatgaccaga tgacctgttt ctgcggctgt
      241 aagcgcagtg cttatggttt cgtagtcacg catttcaccc agcagtatta catcgggcga
      301 ctggcgcagg caggctctca gcgctacaag ataactcttg ctgtcgctgt ttatctcacg
      361 ctggcttatt atacttttct tattcttgtg cagatactcg atagggtctt caagtgttat
      421 tatatggctg tttctctgct tgtttatttc attgattatg caggcgagcg ttgttgactt
      481 accgccgccg gcaggacctg taacaagcac aagacccttt gttcttgaca ctgtatccgt
      541 gattatctcg gggaggatat taagctcttt gtaatcgggt atgtcaaact caactacacg
      601 gatcactgcc gccattgttc ctctttgctt atatgcgctg atacggaaac ggctgagtcc
      661 ctttacagat accgaaaagt catcatcgcc tgtttcctca tactttgaaa tatctctgcc
      721 ggcaaggctg tatatctctt ttaccagtct ttccgcttcg gcaggaagta tgatatcatc
      781 gctttcttta gttattatgc cgttttcctt atatgacacg gcaagtcccg atatcaggaa
      841 tatatcggat gctttctttt cgactgcgcc tttaagtatt tccatcagtt ccataatttt
      901 attcctttca taccggtata ctgtcgccgt tccatatcgg caatgagttt tcgtcttcgt
      961 aataattgtt gcttactgtt ttccagcctg taacgctgag tgttccgtca ttgtactgcg
     1021 ctttgcagat aagcttgctc tggtcgttta tatcttcgct ccacgagaat gtaagtgcct
     1081 gatctccgcg accgccgccg gttacttcaa agccttgctc ttttgccgcc gccgatatat
     1141 cgatcactgt ttcgtcactt gtgatcgtat acagcttgtc tagcctttcg gtcgccctta
     1201 catcggcggc ataatactct tcctgtgctg tgctgagttt ctttgctctg tcaagatcct
     1261 gtcttaccgt aacaaggcac aataccgaca gaataacagt cgcaagcacc acgaatataa
     1321 caagcagtga cgatattccc acgctgttaa ggctttttct gcccggtttg cttttgctgt
     1381 tattcaaggt cagccctcct tgcgcttgtt atagtgatgt atgtatagtt gctgtcggac
     1441 gcagtgatat caagataccg catactgccg tcaatgcgtt cggtcagctt tatcttgata
     1501 tctccgtctg cgtaaagctt ttcaaggtct gccttatccg ctccgctctt atagcactcc
     1561 gctatgtcgg cggtctttat ctgcgcatcg gacaaccgct cggcatatct catttcgctt
     1621 gccgctattc ccactactct gacgcaggcc gccgtgcaga tcgcaaatac agctattact
     1681 ataagcaatt caaccaggaa cgcgccgttt ctggaacgtt ttttcacact catacgctga
     1741 ccgcctttcc gccgcgcagg ctgtaatgtg atgaggtcaa aacaccgtca tacgttattg
     1801 taaagcagat cgtactgccg tccttttcgg cggtaaagcc ttcggcgggc agaagcgtgt
     1861 tgccgccgtc cgcattaaaa ggcgcgtcat acggcacaaa caactctttc agctccccgc
     1921 cgtatgcgta tatgtatgtt atatacttat cgccgtctat catatcggta aagccgagat
     1981 aagctatatc ccccgatttt ccgacagata tatccgcgtc atcatagctt ctgagcttct
     2041 gaagcaccag cgtctgtgcg gtagctgtat caaagcgctt tcctatgtca tttcccagcc
     2101 ttttatacac acttgcaaac gcaagcaccg atgccaaaag acagcatacg aacaatacga
     2161 atacagtcag cacaaaaacc atatcaatac cgcttttatt cgatttt
//
LOCUS       DAWAOG010000353         2203 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6452_length_2203_cov_2.136406, whole genome shotgun sequence.
ACCESSION   DAWAOG010000353 DAWAOG010000000
VERSION     DAWAOG010000353.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6452_length_2203_cov_2.136406"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          572 a          532 c          405 g          694 t
ORIGIN      
        1 ctttgccaca tcatccgccg taagctggca ctcattgaga tagctcttgt caataagccc
       61 atcatcgaca aaacgtttga tcaaatcata atccgcacag ccttcgtcaa atgcgctaaa
      121 agaagctgtc cagagcttat tttcggagtt ataccagtat aacacattct ttttcttttc
      181 ctgtatggaa ttgattgtgt actttaattc atattcttct ttatcgcaat tatattcata
      241 atgaacatcg tactctatct gcggtacgga tctcttcagc aggatattac tgtatttgtg
      301 atcctcatat tttgcaccgg gcacatagcg ttcccagtca tctatgtatc ttccgtcaac
      361 ccatgtgtcg gatacatatc caaggtcgcc gaaccagtaa atttcagagc cgttatcacc
      421 cgcactgtcg gtatagaatt cgttaccgtc accgctttta tacagatacg tatatgtcgt
      481 acccgttgca ccgaatatgc ctgcaatttt tatcagacgc acccatgcgc cgtcaccgac
      541 agtatcgcat atgttttccc atgtgagcgc atcggaacgc gggatatcat ctttcatttc
      601 ggtcttggca taatcatcaa gaagctgctt tgagccattg agagttattt ctgttccatt
      661 gctaccaaat ggaaagtact tctttatctt atcctcgctt agacccttgc cttcaccgtt
      721 tccctgaccg ccgtaagaat aagtttcacc gttatatgta acgtcgacaa gataatgatt
      781 atctgcattc caggaaatct cagcagcggt gtcaagccgc cgtgcgacaa ctgaaaggac
      841 gctcgggaag ccgagagaat cgtaacgata aggatatatc gcagatgcaa aaagcggttt
      901 acttccttca cggctgtatg gtgactgaag atagaaagtc tgatccttgt gcggtgatgt
      961 tgacattccc caatagtcct taacgcgcgt caattctcca aggatataag agccgctgta
     1021 aaggcatatt gaagaaaaac catcctccac cgcatccata ttgtcaaaga aagaggattt
     1081 tttcgcatat gtatcaataa gataatcgac gctgtccttt agtgcaggga gcttgtattt
     1141 tacattggta tcttcccatg taacatcgta tccgtcatcg tttgctactt gcagtactac
     1201 ttctccgccg tcggtataat aaccgtaaaa tatgtaaccg ccgtcaactc tgcctgtttt
     1261 tgcgttctga tatttgatgt cctgaaaaat agtcggctca tcgtcccatg tatcccatac
     1321 cgcttttatg atactctgtt cttcctcatc atatttagtc gatttatccg taaatctgaa
     1381 agaaagagga tcggggttat ctgtctgtac aaagaaatat tgattgaacg gagcgagaag
     1441 cggagttatc gtatagctgt atcctgccgc attaactgta cccgacttaa tatctgccga
     1501 ttccgcctca gcactcagag tttctcctat ggcagggatc atatcaccta caggtgtccc
     1561 gacaacagtg acagccatca ccgccgacat aattcccgaa gttattctct taagcttcat
     1621 actttatttc ctcccgaatt atccgcatac ataaacagac attgtacgcc attgcgaacc
     1681 tcgccgtttt tgccttatta tttttgcata gcttttttga acggcttgcg taatattcct
     1741 caaaagccgc tccacgcttg aacaaacgtt atctgatgca ttgtgcgtat ctcgcactgt
     1801 ttaataaata ttgaatacat ttgcttgttc ccggattata tttcagtata acactgttgt
     1861 taattgctgt caagtattta cttggtattt atgtaatata aatataaaat cgccgcattc
     1921 tcagatacac aaaaatcggg catcgatttt cacaccgatg cccgttcaaa actattggat
     1981 tttacttttt ccgagttccg ttatctgact aacgtcctcc cgctcacact tccgtaatta
     2041 ccgtaaagcg cagtcttacc gctcatatga taagcgcgaa ccctgaactt gtacgttgta
     2101 ttcttagcaa gtccggcttt tctgaatgta gtagtgttgt tgtttgtaat cttagcaacg
     2161 cgtacccact ttccgccctt gtacatttct actatgtagc cct
//
LOCUS       DAWAOG010000354         2202 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6458_length_2202_cov_2.373544, whole genome shotgun sequence.
ACCESSION   DAWAOG010000354 DAWAOG010000000
VERSION     DAWAOG010000354.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6458_length_2202_cov_2.373544"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          662 a          479 c          523 g          538 t
ORIGIN      
        1 gtacttatgc tgttggttgc aagttgttcg ttaatgggta cgggaagcag taaatcaagc
       61 aattgggata aacttaccaa agaagaaaaa cagtggtata acgataatta cggcaacgga
      121 cagtacgata aatatcagga tgcaataagg gattatcaga ataaacatta aaatcataac
      181 accgccgcag gagttaccaa tgttcctgcg gcggtgttcc cttccccccc ttgacaatca
      241 ttctcctctg tgctaaaata ataccataaa gaaaagctat gacaagaaac agtagataaa
      301 tctgcgtcac agagaaagag cggttggtga aagctcccga caaggtttta tcgaacccgt
      361 ctttgagctg tacacgatga gtgttaccgg tttctcccgt tacagagata caagtgcggt
      421 tataccgaat ttaggtggta ccacggaaca aagtttcgcc ctaagcaaat ttaatgcttc
      481 gggctttttt tatgcccgaa attcaggagg acaacataat gaagcttgaa aaactggtcg
      541 gcgaccgttt taaggaaaga ccctcggact gtcagattga cagccacgca attatggtga
      601 gaggcggcta tatcaagtat atggctaacg gtatcttctc gtcatacgca ctcacaaaga
      661 ggataacaaa gaagattgaa cagatcatca gagaagaaat ggacaagata gacggacagg
      721 aagtacagtt tcctgtcgtt atgcccgctt cactctggca ggagtcaggc agatacgaaa
      781 gcatcggcag tgagctttta cgtttcaccg accgtaacag aatgcctatg gtactcggta
      841 tgacacacga ggaagccgct gtacatcttg ttcgcgaata cggcaagagc tacacaaagt
      901 accccttcat gatttatcag atacagacaa agttccgtga tgaagcacgt ccgagaggtg
      961 gtcttatcag agtccgtgag ttcactatga aggacgctta ctcattccac acatctcagg
     1021 aagatcttga gcagtattac gataagtgcc acaaggctta cgaaaacata ttcagacgcg
     1081 caggcgttcc cgaggtaatc tcggtcaagt ctgattcggg tatgatgggc ggcagtattt
     1141 cgcacgaatt tatgctcctt acacccgtag gcgaggatac aatagcaagc tgtcacgaat
     1201 gtggtttcaa tgcgaatatg gaagccgcag acagcattat tgagaataca agagatgaag
     1261 tttccgcacc tatggagctt gttcatactc cgaatatcca tacaatcgaa gaaatatgcg
     1321 actttttaca cactcccaag gagaagagct gtaaggctgt cgtatatcag agaaacgcag
     1381 acgacagcta tgttgttgta ttcactagag gcgacattga cgtaaacgaa acaaaactcc
     1441 gcaacttctt aggctgtgag attcaccccg ctgtgatcac agaagaatgt ggtcttaacg
     1501 caggatatat aggacctgtc ggtcttcccg aaaatatgac tgttctgttt gatacctcac
     1561 ttcagaatac aaacaacctt tcttgcggtg caaataagga agaataccac tacacaggtc
     1621 ttgacattga ccgtgattgt gcaaatgttg aatatcacga ctttgcaaag atccttgacg
     1681 gcggcatctg ccccaattgc cacaagcact caatttctat ttcaagaggt attgaggtcg
     1741 gaaacatatt ccagctcggt acaaagtaca caaagactat gggtatgact taccttgaca
     1801 gcaacggcga ggaaaagact cctattatgg gctgttacgg tatcggtgtc ggcagacttg
     1861 cggcttcggt atgcgaggcg caccacgatg attacggtcc tatctggcct atgcctattg
     1921 caccttggca ggttcatatc tgcgctgtac gttcagatga cgcagatgta aaggcttgtg
     1981 cagataagct gtatgacgct ttacagaacg caggtgccga ggttatctat gacgacagac
     2041 aggtaagcgc aggcgttatg ttctcagacg cagaccttct cggtgtaccg ctgagagttg
     2101 ttgtaagccc cagaaacctg aaggacaact gctgtgaaat ttccgcaagg gataagagct
     2161 tctcatataa agttccggtt gaagaaaccg cagacaaggt tc
//
LOCUS       DAWAOG010000355         2200 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6469_length_2200_cov_2.030769, whole genome shotgun sequence.
ACCESSION   DAWAOG010000355 DAWAOG010000000
VERSION     DAWAOG010000355.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6469_length_2200_cov_2.030769"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          485 a          585 c          456 g          674 t
ORIGIN      
        1 tctcatagta gaagtcagct aaaggtttct tcttgtggca agtcccagct gcataaattc
       61 gccgctgctg tgtacgttaa gctttacgaa acgttttgct ccgcttcttt gcgccttata
      121 caaatagcgc agcaaagcgc ataaagccct taatgcatta tcctttccgg caagacctat
      181 ttcatctgcc gtgccgccga actgcgcggt tatctccgaa acatcgtcta ttgaaaattc
      241 ctcgttgtca agctgttccg cagagcattt tccatagcgg tttcttataa atgtatatgc
      301 ctgcttacgg tttaaaaact ccgcattgaa cagcagctca acgggggtgt actgtgaaaa
      361 ctgtgcgatt atatcctcat cggtcttgtt tgaattagcc ttttcgactg cgtgtacttc
      421 acccgtcgag atatcggcaa acaccatacc tatgccgttt tctccgacat agatacacga
      481 aatatagttg ttagcgccgt cctcaagcat agacgcttct attacagtac ctgcgcttac
      541 aagccttact atatcgcgct ccacaagacc ctttgccagt gcggggtcgg ttgtctgctc
      601 gcagatagcc accttgaagc ccttatctat cagcttcttt atgtaaccct cggcggaatg
      661 atgcggtacg ccgcacatag gcgtacctgc tcttgatgta agcgtcagct caagctcctt
      721 tgacgctacc attgcgtcct cgttgaacat ttcatagaaa tcgcccagac ggtagaataa
      781 tatataatcc ttgtaatttg ccttgatctc agcgtactgc tgcatcatag gcgtgagctt
      841 ttctccactg tttccggctt tcaagagctt cattcctttc gggtttattg attgaattta
      901 cgatgctgta ctgattaggg gcagcctccg caggtcgagc atgaacctga gcagccgcct
      961 gtctgaacct cgcaggtgtc gggatcttcg ccgtcacagc acagtgcgat aatgccgttt
     1021 acttcctgta aaagcttgtc catatcagcc tttgccatag tgaaatcagc catatgctca
     1081 ttctgcatta cgagagtgta taatctctgc atttccttgt tctgtgtgtc gagcttttcc
     1141 ttgttctgct cttcctttga cttgctcatt tcgagcgcaa cattctgacg tgcgagatta
     1201 aattcgccga taagctcctg aagctccata tcggcatcat ttgccttcat tgcctcgtta
     1261 taacgtacat aacgctcgtc tgcctgtata gccttgccga gctctcttgc tgctttgatc
     1321 gcatccattt tatttttcct ttctttccgc ttttgcggct gttaaaaata tatatctgac
     1381 ggggattccc gaccgattgc aaaatttcag tcattaacgc ttacaagctc gcctgtgact
     1441 gcccagggca gagccttttg tattcttacc gtgacgtact ttccgaccat atccttactg
     1501 ccgttgaaat cgactatcac gccgtggcgc gaatgacctg tcataagtcc gtctgcagtt
     1561 ctgccgatgc cctcgcacag aacctcgacc tcatcgccga caaagcgttc gtaagccttt
     1621 tcaccgatgg cattctgcac gtcaagcagc tccctgaacc acttaccctt gtcctcatcg
     1681 cttacagggt cgggcatagc cgccgccttt gtgccctcac gcttactgta gataaaggta
     1741 tacgccgaat catagcgtac cttcttcata agctccacag tgccgagaaa atcctcgtaa
     1801 gtttcgccgg ggaagcccac tataatatct gtagtcagcg ctaccttcgg tatgcgtttt
     1861 tttgcatatt ctataagctc cagataatgt gccgtgtcat agtggcggtt catttccttt
     1921 aaaattctgt cgcttccgca ctgtaccggc aggtgcagat gatatgatat atgctcgctg
     1981 tctgcgatcg tatcaataag ctcatgtgtg cagtccttgg ggtgagatgt cataaagctg
     2041 agcttatatt ttccgggtat cttatcaagc tcggaaagca gctttgcaaa gccgaaatca
     2101 tattccttgc cgtaagaatt tacattctgt ccgagcagta gtatttcctt atatccgtca
     2161 tttataagcc cctttacttc ggctatgacc tcttcgggtc
//
LOCUS       DAWAOG010000356         2196 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6487_length_2196_cov_2.107894, whole genome shotgun sequence.
ACCESSION   DAWAOG010000356 DAWAOG010000000
VERSION     DAWAOG010000356.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6487_length_2196_cov_2.107894"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          611 a          436 c          567 g          582 t
ORIGIN      
        1 accatgacag atgcgctgag agagcagata aagacgctgt cagagggcgg atacagagtt
       61 ctattgtttg cgcacagcga caatatgccg ccggaagtcg agggcggtgc actgcccgaa
      121 aatataacgc ctgtggcact tgtaagaatt accgactgca taagaaaaga agcacctgca
      181 acgctgaaat attttgcaga gcaggacgtt gacataagaa ttatttcggg cgacagcccc
      241 gttaccgttt ctggagttgc tcagcgcgca gggcttgaac attatgataa ttacgttgac
      301 gcgtctaccc ttactacaga cgaggagctg tgggaggcgg ctgacaagta taagatattc
      361 ggacgtgtaa cgccgtatca gaagcttgag cttgtaaagg cgcttaaagc caaaggtcat
      421 acggttgcga tgacaggcga cggcgtaaac gatgttcttg cactcaaaga gtcggactgc
      481 tctatagcta tgcagtccgg aagcgacgcg gcacgaaacg tatccaatat cgttcttctc
      541 gacagcaact ttgcgtcaat gccgaagata gtaggtgagg gcagacgctc tataaacaat
      601 atcgagcgtt caagcgtgct gttcctttac aagacggtgt attcgtttct ggcggcgctt
      661 atgttctgct ttgtgccgat gggctatccg cttcaggcga tacagcttac gcagataaat
      721 atgttcacaa acggcatacc atcgttcctg cttgctatgg agccgaactt caagcgtgtc
      781 aagggtgagt ttatcgaaaa cgtattgccg aaatcgataa tgcacggact gcttattacg
      841 ttcaattttg tcgggatagt gcttatgcgt tatatctcgg ggtggatgga tataattccg
      901 agagaaacct ttgatcacag catcgaaaca atgtcgacca tatgtatagg ctttgcggcg
      961 ttcgtgatac tgttcaaggt ttgtatgccg ttcaagccgt ggaaaatcgg tctgtttgtt
     1021 ttcctgatag gcggatttgc ggcggatata ttcatattgc gtgattttct gctcgacctt
     1081 aagccgatgt gctttgaaat gatagttatg acaggaatcc ttatgggcgc aactgttctg
     1141 atagaggcgt tgtcctcgtt ctttgaacgg catataactg ataatctgct ggcgttccag
     1201 cgatattgga aagatgtgtt tattaaaatg tcggcgaaaa gaaaagcggg aaaggaaaat
     1261 aaaaatgcct gaaaccaagg ttgaaatatt cagcgacgga gcgtgcagcg gcaatccggg
     1321 tcccggaggg tacggtgcta ttctccgttg gaacggtaaa gaaaaggaac tcagcggcgg
     1381 cgaaaagcat acaacaaaca accgtatgga gcttaccgga gttatcgagg cgctgtcggc
     1441 actgaaatat ccgtgcaagg tgatacttac caccgacagt aagtatgttg tcgattcggt
     1501 cacaaagggc tgggtcaacg gctggaagcg cagagggtgg aaaaagtctg acaatacgcc
     1561 tgcgctgaat gtagatctgt gggagcggtt actgccgctt cttgatacgc atgatgttga
     1621 gttcagatgg ataaagggtc acgcaggaca ccccgaaaac gaacgttgtg acaggcttgc
     1681 ggtaagaatg cgcgattact acagcgcgtt ataataagcc gggaagaaga cgattttatg
     1741 aaaaatgaga tcaaagaata taaggaatat ataaataaac aggcggcaga ccccgataca
     1801 gacaagaaga agcttgcgga agagcttctt gtcaggatag gcttttatca gcacgaaagg
     1861 cttatccatc tcatagtgac gatgagcttc ggcgtgtttt ttttgctgtc gctgatactt
     1921 gtttctataa aggtgtattt ccttgcactg tcggtgcttc tgcttgtact gcttgtgccg
     1981 tatataggtc actattattt ccttgagaac tccacacagg agctttataa agtatattac
     2041 tcgctgattt cggaaaaata atccttggtg atgtaaaaaa gttgtgatca tttaagcaaa
     2101 aatcacacat aaccgtgaat ttattaccaa gtttcgttta atcctacaaa attattttgt
     2161 aaatatggat tttttcggct ttctgtagta taatta
//
LOCUS       DAWAOG010000357         2195 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6492_length_2195_cov_2.116822, whole genome shotgun sequence.
ACCESSION   DAWAOG010000357 DAWAOG010000000
VERSION     DAWAOG010000357.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6492_length_2195_cov_2.116822"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1694..1703
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          707 a          448 c          455 g          575 t
ORIGIN      
        1 gctatgggtg acgataaaat tcagtgctca aagttcggtg ctgtaaagtc ttacgattcc
       61 tgcaagaagt ttgtgtattc gcctcttaag cgtattccta agaaagaaat acagcttgca
      121 aactctgatg taaacaacat caacttctga taaaatcata atatttgaag taacagccgc
      181 gacctgtgta agggtcgcgg catttctttg cgtaaatatc catataatca agctgtttac
      241 gcaattttta tcttgatcag catcaaaaaa cgtgcacaaa atgcaacgat atcaagcaga
      301 aaaaagcatt gtcgtttttg acattcggtg gtatagtata aataaattta aacccgatat
      361 aaaaaattgg aggtatttat ggctaatttt aaacgaatac tcgccggtgt aatgtcctgt
      421 gtcacggctg tttcacttgc tgcctgttca aacagtgcaa caaacggctc cggcgcggga
      481 gaaagcgata cgacaaattc aagtacagaa acaactatcg atgacgaaat cgaaaaccct
      541 gtaagtgtcg gcgacataag cattgacgca ggcgaagagg tagagcctgc cgagcttgaa
      601 tatctcggtt gctacgacat aacaacagcc ggtgacgtaa agccgtcata caagtatttc
      661 tcagaaaatt acggctgcac gataaagtgc acaatagtag gttctcttca gattcaggaa
      721 aagctcacaa ctgcgatttc ttccggcgaa tctcccgacc tcgttgacta tgcagacaac
      781 acattccctc tgcttatgtc aaagaatatg tacactccgc ttgaagaata tatggatctt
      841 tcagcaccgc agtggtcagg acttgagcag tacataaacc agtacaagtg gggcggaaag
      901 aattactact acccctgggc atataatgta gctccttact tcctgatcta taacagagcg
      961 gtattcgagc aaatcggcat cgaagaccca aaacttcttt atgacagagg cgaatggaca
     1021 tgggaggcta tgaaggacga tatccgcaag ttcatagact cagatcccga cggcaagaga
     1081 acaggtcttt acggcgcaat gcagtactcg gcacagtcgt tcatcaacag taccggcact
     1141 cccttaataa caattgatga gagcggcaag cttgtacaga actttgacaa tgcaaatgta
     1201 gacagagccg caagctttat gcaggaaatg aaaaaagaag gtcttgcatc attccaggaa
     1261 ggcattatag atgttgatac aacacctatg cttgagggta cggcagcatt ccagtgtatg
     1321 ggcgaatgga ttatctcggg ttatgcaaaa gcaatgctaa aggacgacag ccttgactat
     1381 ttctttgttc cattcccgag agatgttgaa gctgatgaat attactatcc tataacaaca
     1441 ttcggctatc ttgttccggc cggttcaaaa tatgcaaagc aggcttctgt gttcataaac
     1501 tgctgtcgcc tgagcaacac ggacgaagac ctcaaggcta ccactaagga aagtataatg
     1561 agaagcaaga aatacacaga tgaaatgtac gatttcatat acagcttcaa agaacccaca
     1621 aacttccact gcgtagtaga caacccctat ggtcttgacg aaacaaccgg tcagatagta
     1681 aaggatatcc tcgnnnnnnn nnnatcctcg gcaatacact gtttgatatc cagagcgatt
     1741 attcgggtca gtcgtggact cagatgagag aagccaatat cggttcaatc agcaagcaga
     1801 tagaatatta taacggatta ctcgctcggt aaatataatc taataacaca ggccccctgt
     1861 aaattgctca cagggggcat tttagctaat aaagtaaatc ataaaattgt tttattaaac
     1921 aggctatttt aggcactttt ttagtttaca tttttaattt tcatatgttt cattgtacat
     1981 attgcacaat tttttcaaat caactttgac taaaatgtga attgaataaa tgtacttatt
     2041 gtgataaaat cataacaaag atgtgtgtaa acgattgcaa cacattacga tatggaggtt
     2101 tttatgatca atttcaaaag aatattcgca ggagtaatgg cagccgctac tgctgtttca
     2161 cttgcggcgt gctctaacag cggaacaagc acaac
//
LOCUS       DAWAOG010000358         2184 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6545_length_2184_cov_2.024425, whole genome shotgun sequence.
ACCESSION   DAWAOG010000358 DAWAOG010000000
VERSION     DAWAOG010000358.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6545_length_2184_cov_2.024425"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    368..377
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          524 a          610 c          446 g          594 t
ORIGIN      
        1 ctcgataata gtagcaacgt gatcacatag aatagcgaac ccgagaagtg ctgcccaacg
       61 aatagcacta tcggtagcct gctatcatag caaaaacatc gaacaatata atagaagcat
      121 acaaataaca tactttttct acaaacaaaa ccaacctcac cgtttcatac agtgaggtcg
      181 cttttatatt ccgttattcc tacctattct cccttagccc tttcaagcac atctatcacc
      241 ttatcaagtg acggtgcaac cgcatacaca gcattaagct gttcctcctc aggctgagta
      301 aggtcgggca ccattacggt gacacagcct gcactgcttg cggacttcac gccgttgggg
      361 ctgtcctnnn nnnnnnngcg ccatacactg tgacggctca agtccgagtc tttcgcaggc
      421 ataaagatag atgtcggggc agggcttgcc gttttcaagc atattgccgc agattatcgt
      481 ttcaaacttg tcgtacacgc ctatcttctt aagatactcc tccgcacgtt ctatattggt
      541 agcggtagcg accgcagtcc agtagccgtg tgttgtcaga tagtcaagca gagtgtcaag
      601 tccctgcttt ttctctatgc cgtgtacgtc cgtgtattcc ttcatcagct ttacacgcag
      661 gtcgtgtatt gccatatagt cgcagtcctc gccgaaccac tccttaagct tcggtattgc
      721 gaactttctt gaaaatgacc tcagtgcaag cgcgtgctta cgctccatag gaaagccgag
      781 cttgtttgcc gcttcgcacc agtattttac aagaagcttc tcggtatcga gcataagccc
      841 gtccatatcg aatattacag cttttatctc cataggtact cctttattca ggtatagaaa
      901 agtcgttttt gatatagaaa cttctccaac cctaacccca aaccccttcc cccggggaag
      961 gggctgtatt attggggcta ccgcccctaa tccaagcaag gatgcttgca gtcagccaca
     1021 caaagccact gcacgatgca gtggcacaaa tggctgacga cctgtacggg ggctttgccc
     1081 ctgcgacccc ttttatagcg aaatgctact ccttatatct tcgtcatctt ggcaaacttt
     1141 gcggctacct ttttagtgcc cttatcgtca aaagctatct ccaacagcca gtcgccgccc
     1201 atagcctttg ccgaaagtat cgtaccgtca ccgaatactc cgtgatgaac acgctcaccc
     1261 ggtgcatagc ttacattctc ggtgcgcttt tctatctcac gctgctttgt gcggtaggat
     1321 tgctgttgct gctgaagata accgatatgc ggcttcatct gctcggcgct ccttgccgca
     1381 gccgatgcag ttatagccga atgatcgtca taagccatat actgtgacgg gatctccacc
     1441 acaaagcgcg aaaccttgtt tctcattgtc tgaccgtaca gcatacgctg ttttgcgcag
     1501 gtaaagtaaa gatggcgctt tgccctggtt atagcaacat aggcaagtct tctctcctcc
     1561 tctatctcca tagggtcaaa ctgtgcgcgg tatccgggga aaatattttc ctccgcacct
     1621 acaacaaaca cggtgtcaaa ctccagtccc tttgccgagt gcattgtcat aagtaccgtc
     1681 ttatcgtcat cggcttcata gctgtccata tcggaaacaa gcgctacctg ctccagaaat
     1741 tccgataatc ccgcgccctc gttttccctc gcgaaggtta tcatattcga ctttaattcg
     1801 ttgatatttt caagtcgtac ctcgccctca agaccttgtc tttgcatagc ctctctgtag
     1861 ccgaaacgat caagtatatc gtcaataaga cttccgtccg aaatatcatc ggtgtgctgt
     1921 gacagctcat agaacgcctc gccaagcggc acaagcacct ttgcctttct tgacagtgaa
     1981 ggcaggctgt cgctttcgca cattgcctgt acataggata tacccatacc cgatactatg
     2041 cggttgagct cgtcaagtgt cgccgcgcct attcctctta caggctcatt tacaattctt
     2101 ccgaaacgaa cctcatcata gttattgttg agaacgcaca gatacgcgat catatccttg
     2161 atctccttac gctcatagaa gcgc
//
LOCUS       DAWAOG010000359         2180 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6560_length_2180_cov_2.204706, whole genome shotgun sequence.
ACCESSION   DAWAOG010000359 DAWAOG010000000
VERSION     DAWAOG010000359.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6560_length_2180_cov_2.204706"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    625..689
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          677 a          445 c          511 g          482 t
ORIGIN      
        1 cgataacggt acgatactca caaacggcag tatcacaaaa gccgacaatg cggcacagat
       61 aacaaagacg aacggcggcg ttatccgcag gttcacacct gcttccggtg ccggatatac
      121 gctggattat gcaagcggta agtgcattgc ggaaaccggc tataaaataa agctgtcgga
      181 cggaaatact ttaaccgatg ttgccgatgc cgacacaata gtatcggaat caaacagact
      241 tagtattgtc cgcatcggtg acagtgaaat aatagatgac agcgagccgc aagtaactgt
      301 aatgccctat tgcataaagg caaataatac gaatgaaaca accgtaacgg acggaaatgt
      361 ctttgctatg agaggagctc cttcaaggtt tgcgtttgct ctgaacgatc ccgatgaata
      421 ccgtttctca ggcgttacgg taacagatat tgacggtaat gcggtacctg acaatatgta
      481 cacgtttgac agcacgacag gtgagttcat cgcactttcg gttgaccgca accttatcgt
      541 aactacagac gcaagtctta aagatgttga aatatctgta acagacagtg aaaacgatcc
      601 tgtgtcaact gtcgacttcg gtacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng acagtaacgc tcaaggtgaa agtcacagag
      721 ggcggttcta tagcaaagta cagagtagta aaggaatccg accttaataa agcaagcggt
      781 gtcataactg atacttcttc gttcacagta tcatacactg ccggcaatga atggggtact
      841 tccgatgacg gatcttcaaa gattgcaaag ggtgaaactg caacattcac agttaagccc
      901 gacagcaatt taaccgcagg aacttatacc gaagatttta tcgttataac agacagaacc
      961 gcttatgcgg atgcgtcggc aggcactcag gataaggctc tggcacgttt cacgctgact
     1021 tataaggttg aacacgagag tgacggtaat ctgcattatt ccgatctaac cggacactat
     1081 aatctttgta agaactgtca ggcaaaaatc aataaagtaa atcatacatt tgacatcaga
     1141 actgtaaacg aaaacacgtt agctgctcct gcggattgtg taaatcctgc caagtatttc
     1201 tattcgtgcg aatgcggggc gcacacaaat gacacgacgc tggtgtttga aaacggcgat
     1261 ccgaacggtc acacatttgg ggagtgggaa gcagatatac ctgcaggatg ccttacatcg
     1321 ggcagagata aacgcaagtg ttctgtgtgc gaatatgaag aaacacgcga aatcgagccg
     1381 acaggtcata tatgggaaga cgactttacg attgacatta agcccggttg taccgaaaag
     1441 ggatcaaagt ctgtgcattg ctcaaaatgt gatgagaaaa aggacattac cgaggtaccc
     1501 gcaaatggtc attcgttcgg tgaatggaca atactcaata tgacagactg tacacagggc
     1561 ggcgtacagc aacatatatg tacagaatgt ggctttacgg agatctccga cattccgcct
     1621 caggaacatg tatgggatga tgactttacg gtcgatttag caccgacttg tacgcagagc
     1681 ggctcaaagt cgatccattg ctcaagctgt gaagctgtaa aggatatcac tgagatcgag
     1741 ccgatagatc atgtattcac ggaatggtcg gtaagttctg atgccacctg cgtcgaaaaa
     1801 ggacaaaaaa caaggtcgtg cacaaaatgc ggttacactg aatatgatag tatcgatatc
     1861 gatttaaatg cacatttatg ggaagatgat ttcacggtag acaaagaacc gacctgtacg
     1921 gctgacggtt caagatctat ccattgctca cggtgcaatg caactaaaga ttcagaagct
     1981 ataccggcta cagctcatca gtacggcgaa tggaaagtag tcaaaaaagc tacctgcaca
     2041 gagaacggca agcgtgtgcg tgaatgtgca aactgcggaa aaaatgaaga agatatcatt
     2101 ccatcggctc atatatggga taaaaagata actgttgata aagaaccgac ctgtaccgaa
     2161 gacggttcgc agtcgataca
//
LOCUS       DAWAOG010000360         2180 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6561_length_2180_cov_1.929412, whole genome shotgun sequence.
ACCESSION   DAWAOG010000360 DAWAOG010000000
VERSION     DAWAOG010000360.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6561_length_2180_cov_1.929412"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1252..1261
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          520 a          559 c          345 g          746 t
ORIGIN      
        1 actttataca atcagccgta aaaagctgtt taaaaattct atttactgca tttttctgct
       61 atttaatgta ttttatcagc tttttacccc cggctttttc ggatactttc cggtaaggat
      121 tctctccctt atgtatacca gcgtgtcctc ctcgccgtat cttttcaaca ttatcagcat
      181 ttttcccaaa agctccatgg tttccggatg catcatatag tgttttctgt tcttgttgaa
      241 atattcaaac ggcatactgt cattataatt ctctttataa tatgtcttgc acgccgctat
      301 tctgtcacag aacatttcca ccacatactt gatcggcatc ttagtgccct gcattcccgc
      361 accgggctcc tgtccgtaat ctatccagta ttcaaagtga tgtctgtttc ttcccttatg
      421 gtgaagccat gcctcacttt tacctgtcac acgtctttcg gtcgtattcg ggcttttatc
      481 tccggtatag aacacgaccc cgcttataaa ttctttcggc gaatatttcg acagatcgtg
      541 taatattccc tgtttataaa ggcccagctt aaagcacaat ctcatcactt ccatcttatg
      601 atgattgatc gtgcttaaat gtcccttgaa attctcctta atatccacgc ttatccttct
      661 ttcgttcttt cgagatactt tctgaagtta tcgatatatc ttgttttaaa gctgacgctt
      721 gttatttctt tgtatggaat tttggtttct gcaagccatt ttccgtcagc gtcaaactct
      781 ctcatcgtca gtttattctt ccccgcttta atgattattc ctatatggaa tttcccctcg
      841 ttttcattct cgactattcc ccattcaccg cactctctga gcgaattgaa tatgctctgc
      901 cacgagctta tatcaatttt gggagccttg atttgctctg caaccccatt cagtctgttt
      961 atctcttcac agacatcatt cttgatttcc gcttttttca gttcgtcaag gttgcggatc
     1021 gtatatccgt taagctcaaa gtcgttttct tcctgtccga ggacaaatct gtcgttgaaa
     1081 tcattgatat aataatacca atagcttttg tcgtagttgt aaaagcacct gcatatatga
     1141 ccgttttcaa cgcactccct cagcaacgac attttcttac tgcgtttcat atttttcctc
     1201 actaaaaagc aactgccgca tatatctgtc tcttatacac acctccgagc cnnnnnnnnn
     1261 ncaactgccg catatataat acggcagttt catatttaca aaagccgtca gccttattct
     1321 atcacagccg acctttaatt tcaagtccga aagtcattat cgttgttccg tcaattgtta
     1381 cacgctcaac ttctccccct ctttttcatc gcattcctta ttctccgccg ctttatccgg
     1441 ctcgttaccg ccgtgcttat cctttttctt cttttcttct atatgcgact gatcgcttac
     1501 aacaagaata tccccttgct gaataacact gttaccgttc ggtacgacca tatccccatt
     1561 tctctttatc attatgataa gaagcgtagt cggcaggtca agctccttta ttgtcttgcc
     1621 cttccataaa tgctttgaac ctatctcata ctccttgagc aatatatttc cgctgtccct
     1681 gtacgacgga acactgagta taacatcatc acccgcattt atcacagtat tgcctttagg
     1741 tacaatagtg ctgtcaccgt gcttaatcat gagtacaaga gaattttcag ggaaatctat
     1801 ctcgcttatc ttgtgaccgt tccagctgtg tgtttcggat acgaatatac gcatcatatt
     1861 gaactgtgga gtttcgtcct gatagtcgtt gaacgtcttt aatacactgc tttcgttatc
     1921 aataagttta agccccgttg caaccttcgg cagtaacgtt ccctggaatg caacggatat
     1981 aaggcagacg cacactacta tattgaacag gttgctatcc gtgtacacat cgctcaccgt
     2041 agccattatt gcgaaaacga tggacgatgc gcctctcaga cccgaccacg ctataaacag
     2101 cctgtcagcc catgtatatc cctttgagca aatcgcaaac actgcaacag gacgtgatat
     2161 aaaggtcatc accgcaaata
//
LOCUS       DAWAOG010000361         2173 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6593_length_2173_cov_2.214825, whole genome shotgun sequence.
ACCESSION   DAWAOG010000361 DAWAOG010000000
VERSION     DAWAOG010000361.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6593_length_2173_cov_2.214825"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    887..896
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          524 a          549 c          464 g          626 t
ORIGIN      
        1 cgcagtatcc attccggcaa gctctcccga tgcggtggaa acaaagaatt tcagcatcca
       61 gccggcaaca gtcgtataga acatcataag cagataacag ccgatgaggg aaaccttgcc
      121 gtgtaagtgc caccttgagc ctttcggttc aagctgttca tacattttgg cagggctttt
      181 ctgagccgct ctgcctatgg aaaattccat agtgagtaca ggtacaccga gtattatcag
      241 gaagaacagg tatattagga cgaaaagccc gccgccgctt tgacctacca cataaggaaa
      301 tttccatacg tttcctattc ctattgcaca gcctgcgctg agcagtataa agccaagacg
      361 gctgccgagt ttatctctgc ccatagttcc tccgggtcat taatttttta tatatagttt
      421 gttgaaagaa tttgttttta ttaaaatgaa gtgcagattc tcaagcccta tccccctgcc
      481 ccctttccct ggaaaggggg aaaatacggg gactggcgtc cctgcgacct gccttggggc
      541 tacgccccaa accccatttt taacaacgat agaggttttt caacagcctg atttatatat
      601 ctttattctc agcttttctt tacttctccg ccctcaacaa cgagtgtttc cttgtagtct
      661 gcaccgtatg tgtctgtaag agtagcgtca tagtctgcgt ggaagaagtt ttccttgccg
      721 agcgtcttga tctcatcgtt tatccagtta aggagcgttt cgttgccctt tgaaactgcg
      781 ggagcgatag tatcctggct gccgagtgcg tctataccta cagtatagcc cttgttctgc
      841 tttgcgaatg cgataacctc tgtgttgtcg ttagcccatg caacacnnnn nnnnnntttt
      901 taaatttatt ttgtgattta tgcatcggtt ttcagaacct ctctgtagtc ccacagatat
      961 ttgattcccg aaaccgtcgt aagtatgcag gatatgtaca taagtatgtc acttatcagc
     1021 tgtacgggga aagtttctgt cgtgatcgcg ccgaaaacga caagtatatg cataataatg
     1081 ataacgcata tcgcaaccat tgttgaggct gtcttaagct tgccccatat acttgccgca
     1141 ataactgtct ttttctcgct tcctgcggcg ataagccgta ttcccgatac cacaaattca
     1201 cgggcgacta ttatagccgt tacccatgcg cttgcccagc caagctcaac aaagcagatg
     1261 agcgccgtaa cgacaagcac cttatcggca agcggatcta aaaacttgcc gaagtttgtc
     1321 accatattat attttcttgc tattttaccg tctgccgtgt cggttatgct tgcaacggta
     1381 aaaattatcg ccgcccacag atagctgtag ggcacaatat cgcctgcgag cataaagaac
     1441 gcgaaaaacg gtaccagtat cactcgaagc aatgtgagct tattagccag gttcataccg
     1501 atatcctttc agcattacac ctgctcaccg agcagattgt tttcaagcac gtctgttatt
     1561 ttaacattta taaagtcgcc gataacaggg cgcttttctt ttgtcgattt gaagtatatc
     1621 ataccgtcta tctcgggggc aaagtgcata cttctgccga aatacatatc cgagtagcgg
     1681 tcaaagccct cacagacgac ctcatatgtg ttgccgacca tacccgcgta atattcgccc
     1741 atgcggatct cctgctcgcc tgttattatg tccgctctgc gctgtctttc gccttcatct
     1801 acctggtcgg gcatttctgc ggcgggagta tcctcctcgg cagaataagc aaagcagcca
     1861 aggcgctcga actttatatc gtttacaaat tcggcaagct cgctgaactg ttcctccgtt
     1921 tcaccgggga agcctgtaat aagcgtagtt ctcagcacta caccgggaat ttcacggcgc
     1981 agctttgcaa acagctcgcg caggctctgc tcatcgccgc cgcggtacat attgcggagg
     2041 atttccttgt tacagtgctg tatcggaatg tcgatatagt tcagtatctt atcctctgtc
     2101 tttattgttt cgataagctc gtccgttatt ctttcgggat agcagtaaag cagtcttatc
     2161 cacttgatcc cgt
//
LOCUS       DAWAOG010000362         2172 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6601_length_2172_cov_1.929145, whole genome shotgun sequence.
ACCESSION   DAWAOG010000362 DAWAOG010000000
VERSION     DAWAOG010000362.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6601_length_2172_cov_1.929145"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    746..755
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          509 a          528 c          415 g          710 t
ORIGIN      
        1 ttgttagatg tggatttaat tgtactgctc gatttacaat ataatgtgtt gttgatttaa
       61 tgaattttcg acgaactgag ggcaatgata aaatcactgc cctttcgttt taattattcg
      121 tcactgctgt ttttagcttc tgccatgtca tttttcagct catcgagaat agatttatcc
      181 gacttaagac cgtctaccgt gtattcgccg ttagatgccg aaataagagc atctttaaca
      241 agcggaaatc tccattccag attgcgcctg tcgaacagtc cgaacagctc gccgtttccg
      301 gtgaatgcac cgttgtccca ccatacacaa ggaacaccga tcgtattcat ctttgtaatg
      361 taatatttag cccattccgc acggtatttt tcattatcct tgcttcttgc accgaactct
      421 ccgataatga cagcctgacc cttatcgata aatgtagctt tcaaaagatc ggcaagatta
      481 tcaatgtcgg ttgtaaatcc acccttgcat gctatccact tgtttgcttg ctttgactct
      541 gcaagagcaa aattgtacgg tatataagca tgaaccgaaa ctatcagctt atcatcgttt
      601 tccgggattt taagcgcctt aagtgcgacc tcgctcgatg atgcggcata gcccggtatc
      661 ataaggtgac ggagcttgtt gttaccgtca aggcttctta ttgtagatac aaaatcagca
      721 tccagcttgt ttacgacctc acttgnnnnn nnnnnccgtt ccactcctcg cttgtaccga
      781 ttactctggg ttcgttaagt ccctcaaata taagatgctc gtcataatcc ttgaagtaat
      841 tgccgatttg cgtccatacc tttttaagcc tgtcgcttgc cgcctcgtag ttatcctctg
      901 tgggatagtg ccatgtttcg tgatggatat tgagaataac atacatatcg tttgccattc
      961 cgtagtcaac tacttccttt acacgggcaa accattcatc gcttatctta tactccggag
     1021 cttcgcctgt gctccagtcc cacgttgtgg gaacacgcaa aacattgaag cctgctttct
     1081 tgatctcgtc gatcatttcc tttgtagtat cggggttgcc ccacgatttt tcaattgatc
     1141 ccggattctg tcccctgttg ctttcgtttc ctgcgtcaag cgtgttgcca aggctccagc
     1201 ctatcttcat atcctttaca agctcaaccg agcttatatc cttgatttct ccctgatgag
     1261 ccgttgacga gccgttgctc gtattgtctg cggattgcgt tcctgatgta ttgctgtcac
     1321 tgcatccgct tattacaagt gaaccaagca gcattgtaca cgataacact cctgctgccg
     1381 cctttttaaa tgaaaacata ataacactcc ttatgataac ttgtccgttg tgtggaactg
     1441 ctttaaattg cctatctttt cgttgcgctc gctgagaacc tcttcgacct cagaccattc
     1501 aagccccctg tctgcacaga gcaccataac gtgatataaa aggtcgttta tttcgttttt
     1561 aagctcgtca ttgtcggcgt tctttgcggc gatgattgtt tctgcggctt cctcgcctac
     1621 cttcttgcat atcttgtcta cgcccttgtc aaaaagatag caagtgtatg acttttcagg
     1681 tgcatttccc gcgtcatact gtgcctttct tgcctttatc acttcaaaaa tttcttcata
     1741 aactgtcatt tcatacctca tcatttttgt atatttcttt aaagaagcag gtgtagcttc
     1801 ctgtgtggca ggcaggacct gcgggtttta ctctcacaag cagtgtatca tcatcgcaat
     1861 ctgcgtgaat gtcgattacc ttctgcaaat ttcccgatgt agcacccttg ttccagagct
     1921 cctgtcttga acggctccag aaccatgtat agccggtttc aagcgtcaac ttaaggctct
     1981 ccttgttcat atatgcgagc ataagcactg cacctgtgtc aatgtcggtg acaattgccg
     2041 gtataagctc ggctttcttg aagtatttat caagatccat ctgatttctc ctttgtaagt
     2101 aataatctta ttataatacc gattgatgta aaaagcaata gtaaacagga aaaacgggag
     2161 gttataagtc tc
//
LOCUS       DAWAOG010000363         2156 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6660_length_2156_cov_2.139457, whole genome shotgun sequence.
ACCESSION   DAWAOG010000363 DAWAOG010000000
VERSION     DAWAOG010000363.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6660_length_2156_cov_2.139457"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          516 a          459 c          424 g          757 t
ORIGIN      
        1 gggataattc gcgcctgtga ctgttatcca taatatgccg agcaacatta gaaacataat
       61 aggtattccc gttaattttc ctgtaagtat tctgtcgatc tttctgtcac gggataattt
      121 tctgttgcac gattttttta cgctttctgc cgctatacct tcggcagtaa tgaatataca
      181 tgacactatt tcatcggtaa acggtttttc cgattttgat atcagctccc cagccttaag
      241 cagtaaaggc tcgtatttct gcgagcatat cccgaatttg ctgcacaggc tttttttgat
      301 atcatcatcg ttttgcagta atctgagaca tatgaaacgc tgttttctta tgtcatcggt
      361 aattcctact gctagcggca taagctctga tatcgctttt tccgtgcggt ttgtgtagat
      421 aactgtgata tcgcgatttt tttctttgct cagcgctaca ctttctatta ctgccatcag
      481 tttatccaga ccctttttat tgcgagccga ggtcgcaacc acaggtatgc cgagcaattc
      541 cgacagtttt tttgtattga ccgctatatt cttttttgag gcttcatcta tcatgttgac
      601 acacatcacg gctttatttg tgatctcggt tatctgaagc acaagattga gattacgctc
      661 cagacaggac gcgtcacaaa caattactgt tacatcgggt cttgcaaagc agatatgatc
      721 ccgtgccgtc acttcttccg cagaatgagc acacaatgag tatgtaccgg gaagatcggt
      781 tatttcgtac ttgttgttat gatatatata gcttcctgtc gcattaccga ctgttttgcc
      841 cacccagttt cctgtatgct gctttagacc ggtcagagcg ttgaaaaccg tgcttttgcc
      901 gacattcgga ttgcctgcaa gtgcaaccgt aaattcttta tcgtatttca tatttttcac
      961 ctttcagcta atgtaataca ttatatgaaa atacgaagga aatgttaaga gcggagcaga
     1021 gcacttatcc ggtattatca ggatagcaat tcgttcaggg tttctacaag ccgttttgtg
     1081 ctgaccggct ttgtcagaaa tgtattcata ccgtattcag cggctttgtt tctgtcttca
     1141 ctgaatacat ttgcggtcat ggcaataata ggtacatccg gtgcatatcc gttatcaagt
     1201 tgtcttatgg cgcgagaaca ttcgtaacca tccatgatcg gcatctgaat atccataatt
     1261 atgcagtcgt aatagccttt atcgtgagat tttatcatat ctaatgcaac tacgccgttt
     1321 tccgcttaag acggaggatt ttacggtact gcttactttg ctatcttcta agttaaaatg
     1381 tgatgatatt atttttctca gctcatatgt cagaatcggc acagtacata tctgtaccga
     1441 gtgtgacaga gagcatggaa ttttactgcg ctgtgcacta tcggtgacgg caatatacaa
     1501 agtgtctgta tccccttttt caaattcggt tattctgtcc gggattgttg ctataatatc
     1561 ataccgggta cattgtcttt gttttgtgat actgttttct atatcagtat gtattcccag
     1621 ctttttaagc gcagtacacg tttcctccct gattttactg tctgtgattg ctatgaaagc
     1681 agaaagttca ggaaatggtt tatttacgcc tttttccgta atgtgaaaag gcagacgtac
     1741 tataaattca gtacctttcc ctttcttggt atttacctta atagtgccgc ctgcggcatc
     1801 gactatattt ttggttatag gcattccgag tcccgttccc tgaatgttgc tcgttacctt
     1861 tcgttcacgt tcaaaaggct cgaatatttt attcttaaat tcttcgctca tacctattcc
     1921 gttatcgctg acacgaattt catatattga tatattatct gttttgcttg cttcttcgct
     1981 gatgtctagc gtaataatac cgccgtccgg tgtgaatttt acggcgtttg aaagcagatt
     2041 gttgagcagc tgttcaagcc tgcggcggtc acaacgcact gtttcgtgaa ttacttgtga
     2101 catagagata gtcaatttgt gcttcttttc aatcgccaat gtgtacatta cagttc
//
LOCUS       DAWAOG010000364         2148 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6685_length_2148_cov_1.990922, whole genome shotgun sequence.
ACCESSION   DAWAOG010000364 DAWAOG010000000
VERSION     DAWAOG010000364.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6685_length_2148_cov_1.990922"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          689 a          408 c          450 g          601 t
ORIGIN      
        1 ctttatttca gcagactgtt tcataaatac attggtatgt caccaaaaga gtatcgaaaa
       61 gtaaaactca aagaaaatct cagtgagtaa aaaacaaaac tcgtttggct aaaactaaac
      121 caaacgagtt ttgttgattt ttgatcagaa ataaggaatg gctacattac caaacacgcc
      181 tgaattgcaa tctcctcagc ctgcaactca aagagattct caagctgcca aaccttagac
      241 gtgtcggcat ttttcatcgc aagtgcatat tcaggatcag tagcacgcat tttgttccag
      301 atcttccact tttctttttc acactcagtg atacgctcgt caatgtggac atagattctg
      361 ccctacgctg ctaggtgcca cctgtcatga tggaaggaga aagtgaacta cacaactgaa
      421 aatagattac agcagaaaga aaaaacgacg aaaaatcagt gataaaaaat tagtgtttta
      481 gccgtcgcta tttcagcaaa caatcgcaac aaattacgat tgtttgctga aattatatca
      541 tactgtattg taagcaggga ttatttatgc tataataagt tacaatatat actaattatg
      601 gttaaagatg aagtgtcgcc gccttttctg caaagaatct gcgaatatcc ggagagtaat
      661 atcgggagat atgcgacttc attccagttt caaaatcgga ggtgaatgct tgatgacggc
      721 acagctattg tttgagtgcg agcaatatat tcaaaacggc ggcattctta ccggttcatc
      781 ttttgggaga atagaaatac ctttccataa gatgatggac attgataccg atgccgcaga
      841 acagtattta cagcaacaca gggagaatta ttttacagag cagatgtttg cctttattga
      901 tcgtaccggt cagaaagaca gtgatatcta taaaaaagca ttgattgacc gccgattgtt
      961 ttcaaagata cgttcaaaca aaaaatacat acctgccaag agaacggtaa tcgctttgtg
     1021 tcttgcattg gaattaacaa gagaggatgc ggataaactt ctttcctctg ccggatacag
     1081 tttatcccgt gccgacgact tcgatcttgt gattgccttt tgcattgaaa agaaaatatt
     1141 taatttcttt gacatcaacg atgtgcttgt tcatttcggt tttgagccgt tttgaaaaag
     1201 aaaggccacc gtcgggaaac ggcagccggg tatcattttg agtccgcgga tttatcatcc
     1261 gatgtgttta ggttatcgag cgcatattga agacactgca caacgatttt attcatggaa
     1321 gtgttgtact cgccggcaag gcggtcaagc tgctccacaa tatttacggg aatgcggaat
     1381 gttttattga cggattcaaa acccttttca agctcaagca tgataaatct cacctctctg
     1441 catattatta tgcactatat tcagaaataa atatacaagc agtttatctt gcaaaacgct
     1501 tgcagatagt gccgaaaaaa tgtataatga tacttggaga agtgtgacta tcactgagtt
     1561 tcaaaaatgt cgccgctgcg gcgaccaaat tacaagtaat tcggtatata atgattttcg
     1621 gaataggagg gcgactttac tatgagaaaa ccaaagaaat taagaaagct gatcaaagcg
     1681 gtgaaaatgg gaaaaccatg tgctatgtac cgacttggta tttgctttga aacaggaaat
     1741 atgactgaga aagatttaac gaaagctgcc gaatggatct ccgctgcggc cgaggccgga
     1801 tatgctccgg cacttgaatg gataaaggat tattcttttg acgataacgc cttgattcag
     1861 gcggaaagct gagggaatat atgaattatc tgcaatataa cgaaattctt gataaggtgt
     1921 ccttatacga agggcttgaa aaagacacgc tgacaatacg gcttgaggga aatatctgcg
     1981 agcgctgtcc gtccgatatg cacagatatg atatctcttt ccctgccgaa gtcggcagtt
     2041 actcgattga ttttacttgc tacgcaccga agcacaaata cgagctgatt ataaaaagcg
     2101 ttaacgaaaa cagcatcgat attgccttta acggcaacga ttatgctc
//
LOCUS       DAWAOG010000365         2141 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6717_length_2141_cov_1.459252, whole genome shotgun sequence.
ACCESSION   DAWAOG010000365 DAWAOG010000000
VERSION     DAWAOG010000365.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6717_length_2141_cov_1.459252"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    741..750
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1248..1282
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          610 a          434 c          411 g          641 t
ORIGIN      
        1 gtcagtgtat attaccataa gcaacacatc cttcagagct cttaatcagt aaaactgcct
       61 ctttgaatgt cttaaactgt acatctcaat caccttacct atattacctt gaaagcttta
      121 tataataaac gcttttgcgg aaatccgcga ttttccaatc gctataagtt ttttatatca
      181 ccgtagctgt tttgctcgca atttataagg tcaaaaccct tttattatgt gtgtcgatga
      241 gtaccgtatg agtattatca tcgtcccatt cacttcttac tttatgttgc gagtctcaac
      301 agactgctgt gactcactga tttcttgacc cattggactc gcctcctgtc cgactgtatt
      361 tatatacgct tccgactcgt atatagcggt gaacaaaacc ctctttataa tgttaaatag
      421 tcagcaccaa gatgttttgt tctgtctggg gaactttcct tttccttgtc tatatgatac
      481 cacaagaaag caacaatgtc aacagtttta cagcttaaaa ttgtacaaat tgcacagctg
      541 tgttttgtgt ataagtcaca tagttacaca agttacacat aaaacaggat tttcagctat
      601 gttaataacc taacaaaaaa ggaaatcgac aatttgtacc atcaaggata acactgtgaa
      661 aaaatcataa aatacctctt gacaatacca tgcatttcgt gtaaaattat aaatatagaa
      721 cagaggtctt aaaagaaagg nnnnnnnnnn gaacaaggtc ctcggaaaag gaaggaataa
      781 agaatgtcag aaatcgaaaa tatccccgaa aacgacttag acactgctga gttcattgac
      841 ctcgaggacg aaaacggcga aaccgtttct tttgagctta tcggaagaat tgaagtagac
      901 ggtagcaact attgtgccgt cattcccgaa gaagacgacg aaaacgccga cagcgacgta
      961 tatgtgatcc tcaaagaaat aaacaagaac gatgaagttt atcttgcaac gatcgatgac
     1021 gataaagaat atgaccgtgt tgcaaaagaa ttttttaaag tcttcgacga gcttgacgaa
     1081 gaagacgaat aatcagttat tacactgcct tgtgcgcttc agtatgattt catatgatcc
     1141 gacactcttt aatagggatt tctctccgtt tgcggattgc agacctcccg ctttgcggac
     1201 gaagggagcg tgaaacattc gggggaaagt tacccaccgt acttttannn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nncttcgtcg gtagcgctca tattcgttct cagtcttgtc
     1321 acggcagttg tttaccttat aaacggcaaa atcgacttta cggcggcgtg ggaatacatt
     1381 cctttcggac ttatcggcgc agtgctcgga gcggtattgc ttaagaaaat ccctgacaaa
     1441 ctgataaaaa agatgttcgg aatactgata atagccggcg cattgaggat gcttatatga
     1501 atatttttgt cgggcttata acaggtgccg ctgcatcaat gggacttggc ggtggttttg
     1561 ttctgcttgt atatctttcg atctttacaa atacacctca ggatctggcg cagggaataa
     1621 atctgttgtt ttttctgccc attgcactgc tatctctcat aatccatata aaaaacaagc
     1681 tgatcgacct taagcttgtc ggtaaatatc tgatactcgg acttccctgc gctgctgtgg
     1741 gaagttttgt cgccggaatt attgatgttg aaattctcag aaagctgttt gcggcattcc
     1801 tgctttacat cggcatatct caattgttca gcggcgacaa gaaaagtgaa tttacagaca
     1861 aaagcaagcc tgacaacgaa tgactgctgt caggcttgtt tattactttt tgcctttgat
     1921 attatcccgg taggcttttg actgttgttc aaatttatta tccccgaatg tataataaac
     1981 cctgtctttt atagcatctg gcagatactg ctgatcaaca taatgattgg gatagtcgtt
     2041 cggatacaga taatgctgac cttttatcac gtcgccctct ccgtcatagt gcttattctg
     2101 aaggtgtcgc ggtatatcgc ctgtcttacc atcctcaata t
//
LOCUS       DAWAOG010000366         2136 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6741_length_2136_cov_2.094666, whole genome shotgun sequence.
ACCESSION   DAWAOG010000366 DAWAOG010000000
VERSION     DAWAOG010000366.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6741_length_2136_cov_2.094666"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          567 a          522 c          483 g          564 t
ORIGIN      
        1 cgacagtatc attacttcat ccgtagcata cgattttaca aacgatctgc acaagaggtt
       61 gcttgtcgag ggaacaaagg taaacgacgc tatcaccgac agcttcaacg cacttactcc
      121 taccgtaatg aagttcagat ttgagggcga aaccttcgga ataggactgt ttatgctctt
      181 attcgtctgc atacacgcat tcttcatggt attcttccgc ccggacgcaa agaaatacaa
      241 aggtgctgtg gaggtcgcaa tgggagaaaa cgaaattcat cttgcaacaa cctcggctca
      301 aacggtcaca atgaaacagg cagcctgcga atactgcgga ggagtttata ctgtaggcag
      361 ctgcaacgtc tgccctcact gcggagcgcc tatacagcct caggattata ctgttcccgt
      421 aaacaataat acaagctcga ccgctacaat aagtacaacg accacaaaca cgcagtaaca
      481 attattgagg tgtaatatgg acagctcgca gataataagt tcacctatcg gcattgttct
      541 gctgatcatc ggctcggctg cggcagcagc ggcgcttgtc agcgtgataa ttcagcttta
      601 taaaagtcac gtttcggaca aaagtatgcg ccgcgaattt gccggtacgg ataaggctga
      661 gcaaaagaaa tacagacctg taccgaaaaa attcaaagct ctcgaagaac gtttccggga
      721 gcttgatatt ccgcctgtgt actcgttcac aggcaattgt cgcagtgaga attttatgat
      781 cacggcaaaa cgtgtatgtc tgatctatat ctgtagtcac acattgaacg gtgaagcgct
      841 taataaaaag gtgttcataa attgcgaaaa ggcagtgcag tacattttcc gtgaggtaat
      901 ggatattgtt cttaacagct acggtgaaga ggggtataaa gcgtacacct caaagctcaa
      961 tgccggagaa aggtctatgc tcggtatatg agccgccggc ttcaaattgt atataaacac
     1021 aaaaatcccc tccgcaccgc ggaggggata atgtttttat aacgtaataa ttacttgata
     1081 actcttacag agataacaga ctcgcctgct cttatcttgt cggctacgct gtcagcattg
     1141 cccttgacat caagaaccgt gtaagcatag tccttctttg acttgctctc catattctca
     1201 atgttcatgc ctgcctctgt gatgcaggtt gtgatcttgg caataagacc ttcaacgttc
     1261 ttatggataa cgcatatctt agcatcgccc gccatagcca ttgaaacgtt ggggaggttt
     1321 acactgttca cgatgttgcc gttttcaagg taatccttga tctcgtttgc tgccataaca
     1381 gcgcagttat cttcactttc aggagtggaa gctccgaggt gaggaagtgc aacaacaccg
     1441 tcaacaccga ggatcttgtc gccggggaag tctgtaacat atgctgccat gccgccgttt
     1501 gcgagagcct caacaactgc gtcctcgttt acaagaccgc ctcttgcaaa gttcattatg
     1561 cggacattct tcttcattgt cttgataacg tctgcgttta tcatatcctt tgtttcggga
     1621 gtaagcggaa cgtgtactgt gatgtagtcg caggtttcaa atatctcctt gttgctcttg
     1681 acatacttga ccttgtttga aaggttgagt gcgccctgaa cagaaaggaa cgggtcagcg
     1741 ccgataacct ccataccgag ctcagccgca gcatttgcga ctaaagcgcc gatagcgcca
     1801 agaccgataa cgccgagctt cttgcccttt atctcaggac ctacgaactg gctcttgcct
     1861 ttttcaacaa gcttgccgac ctcgtcgccc ttccccttta aggtctttgc ccagtcaata
     1921 gcggctgtga ccttacggct gctgatgaga agaccgagta ttacaagctc cttaactgcg
     1981 tttgcgttgg caccgggagt attgaatact acaataccct tttcgctgca cttgtcaacg
     2041 gggatattgt ttacacccgc acctgctctg gcgattgcaa gtaaatcatc gccaagctcc
     2101 atttcatgca tagatgccga acgaacgagt atagcc
//
LOCUS       DAWAOG010000367         2134 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6748_length_2134_cov_2.134199, whole genome shotgun sequence.
ACCESSION   DAWAOG010000367 DAWAOG010000000
VERSION     DAWAOG010000367.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6748_length_2134_cov_2.134199"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1009..1018
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          666 a          444 c          531 g          483 t
ORIGIN      
        1 gtcatagccg tagttcttgt cattgttata ttcaactgct tctcgatagt aaacgaaggc
       61 tttatcggtg tcaagtacac atttggaaag ataacgcagg acaatcttgt acccggtctt
      121 aacttctgta taccgtttat tgaagaaata agacaggtgg atacgagaga acagatctat
      181 tcggtaacgg acgacgctta tacaagcgac acacagacgg tacagtcttt acagctcaag
      241 ctcaactaca gatatgacag tgcaaagctg tccgatataa taagaaatgt tggtattgat
      301 aatgttgagt caaagcttct tgttcagaac gttgccaaga tatcgaagaa tgagataggt
      361 aaagtcaagg cagaggagct tgtacagtca agagccgatg ttcagcagac tatacagcag
      421 gagcttacaa atacgcttgc tccgagcggt attatagttg tttcttttgc gatagaaaac
      481 cttgcgtttg acgaagcgtt tgaaacatct atccaggcta aggtcatagc cgcacaggac
      541 gctctcaaga tggagaacaa gacaaaggaa aaagaggaag aggcaaagca ggtcgttatc
      601 gccgcccagg caaaggcaga cagcacaaag cttgaagcgg acgcacaggc ttacgctatc
      661 caggctgtac aaaaacagct tgaaacaagc cccaactaca ttgattatat gaagataaac
      721 agttggaacg gtcaattacc tcagataatc ggtgacggtg taaatccttt cgtaaatctt
      781 gacagcagtg caaacagcac agaaagcaag agtgacaaga gcgacagtac atcaaagagc
      841 aactgacagc ctatcggata agccggtaaa cgaataatga attaaacgtg cccgcagggc
      901 acacctcaat tattcactgt tcattattca ttattcattt aatttcaggg gaggggaaca
      961 ccctccccgt tgttgccgtt taagcgacat taagataaag gggtaattnn nnnnnnnnga
     1021 aaaaggcggt gttccgcata aacggggcag tgcttatagt tgcgggtgca ggaagcggaa
     1081 aaactaccgt gctttgtaac cgtatagcga atatgctcct tttcggtaac gcgtataaca
     1141 gtgacacggt aagagaactg tcggacgaag atacagcgta tgcacagagc tttctggcgg
     1201 gcgaaataaa caactttgac gcggctgaac ggctgtcgga gattttcggc gaggatagga
     1261 taaagccgtg gaacatactt gcggtgacct ttacgaacaa ggcggcggca gagcttaagg
     1321 aacgtcttga aaatatgggc gcggacgttg acggcatatg ggcggcaaca ttccacagcg
     1381 cttgtgtgag aatactgcgt caggatatag aagagctcgg aatgggctat aaatcgaatt
     1441 tcacgatata tgatactgac gaccagctta aggtcatcaa gacggtgatg aaggagcgca
     1501 atatatccga caaggctgta acccctaaat caatacagaa tataatttca cgctcaaagg
     1561 acaaactcat aactcccgat aagttcagca caaagggcaa gaacggaaat gacgattatc
     1621 agctttctac cgcaaagact atctataccg attatcaggc aaggcttgcg gcggcgaatg
     1681 cgcttgactt tgacgatatt ataatgctga ctgtaagact gtttgcacag tgccccgatg
     1741 tactttcgca ctggcagaac aggtttaaat acataatggt ggacgagtat caggatacca
     1801 atgcggcaca gtacaagctt gtatcactgc ttgccgaggg tagcggaaac ctgtgcgtcg
     1861 ttggtgatga ggatcagagt atctaccgct tcagaggcgc taccatcgag aatatcctct
     1921 cgtttgaaga ggagttcggc gctgaggtca taaagcttga acagaactac cgttcgactg
     1981 cgaccatact caaagcggca aacgcagtca ttgcaaacaa cactcagcac aaggacaaga
     2041 acctgtggtc tgatctcggt gacggcgata agataagaat ggatcgtttc tcgaccgagc
     2101 aggaagaggc tatgtccata acggagcgta tcct
//
LOCUS       DAWAOG010000368         2131 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6766_length_2131_cov_2.719653, whole genome shotgun sequence.
ACCESSION   DAWAOG010000368 DAWAOG010000000
VERSION     DAWAOG010000368.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6766_length_2131_cov_2.719653"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          656 a          480 c          520 g          475 t
ORIGIN      
        1 gttctcgcat caccccggac ttcttatgat agcggaagag tcaaccgcat ggcctatggt
       61 aacaaagcct gtcgatatcg gcggtcttgg cttcaacttc aaatggaata tgggctggat
      121 gaacgatatg cttgcgtata tgtcgatgag cccggaatac aggcagtaca accatgacaa
      181 gctgaccttc tcgttctact atgcattctc cgaaaactac atacttccta tttcgcacga
      241 tgaagtagtt tacggcaaat gttcaatgct cgacaagatg agcggaccga gagaaaaacg
      301 ttttgcatcg ctgagaacat tccttgcata tatgacagca caccccggta aaaagctcat
      361 gttcatggga caggaatttg cgcagttcaa ggagtggaac tacaagacgg gtcttgactg
      421 ggatatcctg aaattcccgg agcacgcgca gtatcaggcg tttgtcaagg cgatgaataa
      481 attttatctt gacaacaagt cgttgtggga gatagattat gactggagcg gcttcagctg
      541 gatatcaaac gatgacaaca aaaacagcgt gatcgttttc agacgtatag cctcaaacgg
      601 cgatgagctt atcgccgtat gcaacttcct gccgaacgag cacgagcagt acagcttcgg
      661 cgttccttat tatgcagact ataaggaagt gttcacgaca gacgacaaga aattcggcgg
      721 cgacagtgac ggtaatgctt catataccgc aaagtgcgaa ggaatgcacg gacttgaata
      781 ttcaataaca atgaagatac cggcaatgtc cgcagtgttc ttgaagcccg tcaacaagcg
      841 cagtccggaa agcgacaagc caaagcccga aacggcagaa aagcctgcaa aaaacgcaga
      901 ggaaaagccg gtgaaaaagg ctgaaacaca gacgcggaaa acggcagaga aaaccgtcgc
      961 aaagcccgga gcttcagcaa agaaacctgc ggcaaaaaag acaaacacgg cgaaccgtaa
     1021 gaaaaaacgg aaccgctgag aaataaataa cggaggaact tatttatgaa acatgtaaag
     1081 aaggaatgcg tagcaatgct gctcgcaggc ggtcagggaa gcagactgta tgctctgaca
     1141 aaggacatgg ctaagcctgc cgttccttat ggcggcaaat acagaattat cgatttcccg
     1201 ctttcaaact gtgtaaacag cggcatcgac acggtcggcg tactgacaca gtatcagccg
     1261 ctcgtactta acgaatatat aggtaacggt catccgtggg gtcttgacag agttcacggc
     1321 ggtgtacacg tgttacctcc ttatgagtca tcttcaggca agtcgtggta tgaaggtact
     1381 gcaaatgcga tatatcagaa catcagcttc atcgacagat acaaccccga ctatgtggca
     1441 gtgctttcgg gcgaccatat ttataagatg aactacaaca aaatgcttga atttcacaag
     1501 tcacacaaag cagacgcaac tatcgcagtt cttgaagtgc cgtgggaaga agcttcacgc
     1561 ttcggtatca tgagtgcaga tgaaaatgac attatttacg aatttgcgga gaagcctgca
     1621 cagcctaagt ctaaccttgc ttcaatgggt gtttacatat tctcttggca gaatctgaaa
     1681 aagcacctta ttgccaacga aaatgacgag ggcgcaagca aggactttgg taagaacatt
     1741 atccctgcaa tgctcgcaga gggtaataag ctggtggcat accactttga cggatactgg
     1801 aaagatgtcg gaacgatcga cagcttatgg gaagctaata tggatctgtt aaatcccaac
     1861 gttccgcttg acctccacga ctcaagatgg agaatttact caagaaaccc cgtaatgcct
     1921 cctcactatg caagccctca gagtgttatc cagaactcac ttatttcaga aggctgcaac
     1981 attgaaggtc acatagactt ctctgtactg tttgcaggcg taaccatcga acccggtgca
     2041 gtcgtgcgtg acagtatcat aatgccgaac accacgataa agaagggtgc ggtagttgaa
     2101 tacgctattg tcggcgagga ttgtgtaata g
//
LOCUS       DAWAOG010000369         2130 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6771_length_2130_cov_2.438072, whole genome shotgun sequence.
ACCESSION   DAWAOG010000369 DAWAOG010000000
VERSION     DAWAOG010000369.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6771_length_2130_cov_2.438072"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          687 a          437 c          495 g          511 t
ORIGIN      
        1 tgaaaacctc atcaagatcc ttgaatcaag acttgataac gttgtattca gaatgggtat
       61 ggctatcaca agaagagaag caagacagtt agtaacacac ggtcacttca cagtaaacgg
      121 caagaaggta gacgttcctt cttacagagt aaagcccggc gatgttgttg cagtcagcga
      181 aacaagcaag aagagcccca agttcgctca gatcatcgaa cagacaaacg gcagaatagt
      241 tccgttatgg ctggacgtaa ataaagaggc tatgacagcc aaggttactc gtgaatttag
      301 cagagaagag cttgactatg aaatagctga gcacctgatc atcgagttat attcaaaata
      361 atctcgtcga gcagtattgc gaaaactctt cataccgtgg acagcatggt atgtatcggt
      421 tatttttaat ttacaattcc gcactgagat atgtgcggag ggcattgccc gccaaatcgt
      481 aatttaaaat taaccgggcg gttcccccga aaagggggat acagcccgtt cgtacatcgt
      541 taacccgatg aaaacatctg acaaacttgt gggagggtaa accaaatgct tgaaaaaatc
      601 gaaaagcctg tgatcgagac cgttaagtta aaacccgatg gaacctatgg tatgtttaca
      661 cttgagcctc tggaatgcgg ctatggcaac acacttggca acagccttcg tcgtgtactg
      721 ctttcatcgc ttcccggcgt tgcagtaacg tctgtcaaga ttgacggcgt tcagcacgag
      781 ttttcgacta tccccggtgt taaagaggac gtaacggaaa ttatcctcaa catcaagggt
      841 ctcattgcaa aaatgtatgg cgaatgcccg aaaacagttt atattgaagc tgagggcgag
      901 tgcgatgtta ctgccggtga cataaagacc gacagtgaga tcgaaattct cgaccccggt
      961 atgcatatag ctaccctcgg ccccggagca aagctctata tggagattac gctcgacaag
     1021 ggcagaggat atgtgtctgc tgaaaagaac aagcagcagc ttcagccggt aatcggcata
     1081 attgctgtcg acagcattta tacacctgtt ttaaaggtga attatacagt tgagaacacc
     1141 cgtgtaggac agcgcaccga ctatgacaag cttatattag aggtttggac agacggtacg
     1201 atttctgcaa aagaggcagt ctcatatgcc gctaagcttc tggtagacca tctgcagccg
     1261 ttcgttgagc tgtctgatta ggctatacag cctgagctta tggcagacaa gagtgagtcg
     1321 ggcaaggata agctcctcga gatgactatc gatgaccttg acctttccgt tcgttcattc
     1381 aactgcttga agcgtgcaaa catcaacact gttgacgacc ttatcaataa gtcgcagggt
     1441 gatatgatga aggttagaaa cttaggcaag aagtcgtttg aggaagtcag agcaaagctc
     1501 cagtctttag gttttgatct cgcaagcgac gatgacaaca attgacaagt gactttcaat
     1561 ttgtctgaaa ggagaaatat ccatggcagg ttcaagaaaa ctgggaagaa ccagtgatca
     1621 cagaaaggct atgctcagag gtatggtaac actgcttctt gaaaagggca agatcgttac
     1681 tacagttgca agagctaagg aagttcgcag cgcagctgaa aagatgatca ctctcggcaa
     1741 ggccggcaca ctgcatacaa agcgtcaggt ttacagctac atcactaagg aagacgtagc
     1801 taagaaactg tttgatgata tagcacccaa gtacgctgac aagaacggcg gttacacacg
     1861 catcatcaag atcggcccca gaagaggcga cgcagcagag atggctgtta tcgaattagt
     1921 ttaatttgat ataattaaat agcttacgcc cacctttcgg tgggcgttgt tgtttatata
     1981 aagcaaaata agagcatata taatatcatg atgataaaaa atatcacaca cataaaaacg
     2041 aattgtaaca aaaatataaa tgaaaaactt aacagatata ttcatcggca aatttaacaa
     2101 aaacaataat aaaatttgta taatctaaca
//
LOCUS       DAWAOG010000370         2121 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6810_length_2121_cov_1.836883, whole genome shotgun sequence.
ACCESSION   DAWAOG010000370 DAWAOG010000000
VERSION     DAWAOG010000370.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6810_length_2121_cov_1.836883"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          548 a          464 c          490 g          619 t
ORIGIN      
        1 ctctcttcat gcggtcgagc attgtgtctg caagctcgtc acggttctcg taaggaacct
       61 tctcatcgag gatagaaaca tcgcctgttt cctttatgta agccgcaacc gcgagaatca
      121 tccacagcgg gtcatcgttg aagttagagc caagctcgtg gttacccatc tttgtaaggg
      181 gctgatactg atggtaagca ccgccgtcct caagcattgt agcggcaagg tcgataagtc
      241 tttctcttgc tcttgcgggg atctggtgta cgaagccgag taagtcctgg ttggagtcac
      301 ggaagcccat acctctgccg ataccgctct caaagtaaga tgcggaacgt gacatattga
      361 atgtaaccat gcactgatac tggttccaga tgttaaccat tctgttgagc ttttcatcgg
      421 gagaatcaac gctgtactga ccgagcaggc tgttccagtg agccttaagc tctgcgagga
      481 gcttgtcagc cttttcggct gtgtcgcaca tagccatcat agcctgagcc ttagccttgt
      541 ttacgatgtt cttctttctg tcgccgcttg ttataattgt gctgttctcg tcaaggtcgg
      601 gagcaaactt ttcttcaacc ttgttctcaa catagcccag gcagaataca agatccttgc
      661 tttcgccggg ctgaagcttg atttcgatat agtgagaagc aaccggtgac caaccgtctg
      721 caacagacat attgggtgcg cctgcgaata ctgtctgagg agcgtcaaag ccgttgtata
      781 agccgaggaa gctctctctg tctgtatcgt aaccgttgat aggtgcattt acagaataga
      841 atgcgtagtg atcgcgtctt tccttatatt cggtcttgtg gtagataaca gagccgtcaa
      901 tttcaacctc acctgtgttg aagtttctct ggaagtttgt agtatcatcg tttgcgtccc
      961 acagacacca ctcaaggaag ctgaacagct ttaaattctt ttccttgtcg ctgtcgttct
     1021 ttatcgtaac cttctggatc tctccgttga agttctgggg aacaaagaat gtagctgtag
     1081 cagtaacgcc gtttgatgag ccctttataa tcgtatagcc cataccgtga cggcactcgt
     1141 agctgtcaag cgttgtcttt acgggtgacc agccggggtt ccatacatga ccgccgtcgt
     1201 ttatgtagaa ataacgtccg cccatatcaa cgggaacgtt gttatagcgg taacgtgtaa
     1261 ttcttctcag tcttgcgtcc ttgtagaagc agtaaccgcc tgctgtgtta gagatcagcg
     1321 agaaaaagcc ctgtgtgccg agatagttta tccacggata aggggtctcg ggagatgtaa
     1381 tgacatactc cttgttctta tcgtcaaaat aaccgaattt catagaatcc tcctaatgtt
     1441 tttgtttctg tagcaaaaaa attacaacgt tacagaaaac gtctatgcta attatatctt
     1501 aaatgaattt ttttagcaag ggttgtaatc gttttaaaaa caaaaatttt tgtgaaaagc
     1561 aaaaatccgc aatctgcaca aaaaaatggg ccgtgttagt gcattgtgac gattagtagc
     1621 actatattga tatttggttt cccgacttgc tttacaatat ggcggaaaaa tgatataatt
     1681 attgtattat tgggacgatt ttgtgattat tttaactttg ttccgatttt tgatatgacg
     1741 attttgacaa acgagccaaa ggacacaata tgaaatatat tgaataccga gaaaaacgcg
     1801 agcacggaac tcgtgaattt ccttatgcgt gctacagggt aaccgagaat ttcccgcgct
     1861 ataatatgat tccacactgg cataacgagt gcgagatcat ctatgtcagg agcggatatt
     1921 tcgagctttt ccttgacggt catacacaca ggatagaagc agggtgctgt tcgctgataa
     1981 tcggcggaag tatgcacggc ggtacgccta aagataactg ccgctatgac tgccttgtat
     2041 ttgatatggc ggctttgctt aagtcaagcc agatatgcgc cattcatctg caaccgataa
     2101 tcaatcacga aaagcgactg a
//
LOCUS       DAWAOG010000371         2116 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6835_length_2116_cov_2.161087, whole genome shotgun sequence.
ACCESSION   DAWAOG010000371 DAWAOG010000000
VERSION     DAWAOG010000371.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6835_length_2116_cov_2.161087"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          612 a          495 c          527 g          482 t
ORIGIN      
        1 caacaggcaa gcccaccgtt gtaggctaca caaagaacga ccttgatatg tggtctgact
       61 gcgttgcaag acttgttaca gccgcaggcg ctacagatga agacatagtg cagatcgcat
      121 tcggctacgg acttttcacg ggtgcgctcg gtctgcatta cggccttgaa aagataggcg
      181 ctacagtagt tccctgctca tcgggcaaca ctgaaaagca ggttatgctg atgaaggact
      241 tcggcacaac ggcacttgtc gctacaccct catacgctat gagaataggc gaggtcgcag
      301 aagaaatggg tatcggcaga gatgagctca agctccgact cggtctgttc ggctcagagg
      361 gatgcactcc cgaaatgaga gcgcagatag aagattatct gcatctgctt gcaaccgaca
      421 actacggtat gagcgagctt atgggaccgg gtgtttcggg cgaatgttca ctgcgttgcg
      481 gtatgcactt caacgaggat cacttcctcc ccgagatcat caaccccgaa acaggcgagg
      541 ttctccccga aggctcaaag ggcgaacttg ttataacaac actttcaaag gaggctatcc
      601 cggttctgcg ttatcgtaca aaggatatta cttctatcac ctacgaaaag tgcgaatgcg
      661 gaagaactca cgcccgtatg agcaagccct ccggcagaag cgacgatatg ctgaagatca
      721 gaggcgtaaa tgtattcccg tcacagatcg aaagcgttat tatgactatc ccgcagatcg
      781 ctccccacta ccagctcgtt gttacccgcg agggaagcag tgacagactg gaggtcaagt
      841 gtgagcttgt tgacggaagc gtgcttgaaa gccttgaatc gctgtcgaac ttacagaaaa
      901 atatccacca caatcttaag acggttctcg gcattgatac aaaggtcacg cttgttgaac
      961 ccaagacaat tgaacgtttt gagggcaagg caaagcgtgt aatcgaccta cgcaacaagt
     1021 aatatgaagc tgaaatatga tgaagctgaa atatgatgaa gcggatatgc acgcacagct
     1081 tgtaaaggtg ctgtatcccg atgaaaccgc aatgtttcct gtatactgcg tatataacga
     1141 taaatccttt atgggaagtg ctatggaata cggctattta gctgttacca acaagggcag
     1201 actgctgatg atacgctatg attttatagg cgacggcaac tgcggttcgt gtccgctcac
     1261 cgctataaag aggcttaaga taaaaaagct ccttatcggt cagtacaagc ttgaatttac
     1321 gcttatgacg gacggtaagg atttcaggac gacggtgcag atagcgccga aaatggcttt
     1381 cggagcattc ccacatcaat acgcaaattt taatatgctg ctggacacac tgcgtcctta
     1441 tatagcctga taaaggaaag agagctaaaa tgtcacttga aaaagtaaag gaatatttca
     1501 aggcttacgg catagaggac agaataacag agctgtccga gtcgagcgca acagtcgaac
     1561 ttgccgctca cgcacttcac accgagcctt gcagaatagc aaagacctta tcgtttatgc
     1621 tcggtgacag cccgatactc gttgtaacgg caggcaacgt aaaaatcgac aatgccaagt
     1681 accgccatac attcggtgca aaggcaaaaa tgctgaccgc agatcaggcg gctgagctta
     1741 tcggtcacgc tgtcggcggc gtatgccctt ttgctgtaaa tgatggagta gccgtttatc
     1801 ttgacgagtc gctgaacaag tacgaaacgg tctttcctgc ctgcggaagc tccaacagtg
     1861 ctatcgagct tactatttct gagcttgaaa agtgttcggg atatgagaag tgggtagacg
     1921 ttaccaaagc agtgtcataa tgacacaagt aattcgccgt tctgacagct tgcggcttgc
     1981 gtaacctgca ttaccggcgg gtgcttacgc acaccctctt aacgctttcg gcaaaccgaa
     2041 aatcactaaa agcaaaagca ctgggctatt ccctataaaa agcaatataa aaaggcctgt
     2101 gacgaaatgc aaaaat
//
LOCUS       DAWAOG010000372         2108 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6872_length_2108_cov_1.698490, whole genome shotgun sequence.
ACCESSION   DAWAOG010000372 DAWAOG010000000
VERSION     DAWAOG010000372.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6872_length_2108_cov_1.698490"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    883..892
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1396..1405
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          666 a          446 c          518 g          458 t
ORIGIN      
        1 agcctgtact ggtaggtact atcactattg aaaaatcaga acttctttcc tcaatgctta
       61 agaagaaagg cataaagcac gaggtgctga acgcaaagaa ccacgagcgt gaagccgaga
      121 tcgttgcaca ggcaggtaag aagggtgcgg ttaccatcgc aaccaacatg gcaggacgtg
      181 gtacggatat tatgctcggc ggtaacgcag agtatctcgc aaaggcaaga atgagaaaag
      241 aaggcatgga cgaagcgctc ataaacgagg ctacaggctt tgccgaaaca gataacgaag
      301 atatcctcaa tgcaagagaa acctatatca agtacaacgc agaatataag gccgcaataa
      361 agaacgaggc tgaggaagta agacaggcgg gcggtctgtt catactcggt acagagcgtc
      421 acgaatcccg ccgtatcgac aatcagctga gaggtcgtgc cggtcgtcag ggtgaccccg
      481 gttcagagtg taatgcagac gctcggtatt gaagacgatg ttccgattga aaacggattt
      541 ctcacaaaga ctattgagtc ttctcagcgt aaggtcgagg gcagaaactt ctcgatccgt
      601 aagaatgtcc ttgactttga cgatgttatg tcacagcagc gtatgacgat ctactcacag
      661 cgtgccaagg tgcttgacgg tgaagatgta agcgattatg taaagagcat gatcaaggaa
      721 accatagagg agaacgtcaa aacatactgc tccgatgagt atcccgaaaa ctggaacttc
      781 atcggtctgc gtgagcattt tgcaaatatg cttacaaagg aagacgactt caattatact
      841 acagatgatc tcaataatat caaccgcaag gatcttatca agnnnnnnnn nnccgatgca
      901 ctttatgccg agcgtgaagc aatgttcggc gaggaaaata tgcgtgagat tgagcgtatc
      961 tgtctgctca aggtcgttga tacaaagtgg atggatcata ttgacgcaat ggaagagctc
     1021 aagcgcggca tctacctcag aagctatgct caaagagatc ccgttgttga ataccgtatt
     1081 gaaggcttcg caatgttcga cgaaatgatc gcttcgatcc gcgaggatac agcaagactt
     1141 gtccttacag tcaaggtaag actggaagaa ccaccccagc gtgaacaggt tatgaagcct
     1201 gacgctgaga atgccggctc acacacaacg gtaaagaaat cttcttccga cagaatagga
     1261 agaaatgatc cttgtccctg cggcagcggc aagaagtata agaagtgctg tggcaaagac
     1321 gaataattca gattaacgat gcagtttcag cttgtgttca caaaacgaat acaagctgaa
     1381 cgctttaatt aaccannnnn nnnnnggaga aataggcaag gtctgctgtc cgccctatga
     1441 cattatcagt cccgaacaga aaaatcagta tattgctgaa aatccgcaca atattataag
     1501 actggagctt cccaagaccg caaatgacac agatgaagca tacggcaagg caagagctta
     1561 tcttaacgag tggcttgatg aggagatact gaagtgtgac gataagcccg ccatatacat
     1621 ttatgaaatg gtatttgatg cactcggctc aaactacagc gtcaagggct atgtttcact
     1681 ggtaaagctt gaagagttct caaagggaat aattctccct catgaagaaa cgctttcaaa
     1741 ggctaaagag gacagattta accttatgtg cgcaacaggc tgcaacttca gccagatcta
     1801 ttcgttatat atggacgaag acaacagcgt attcacgctt ataaacgatg caagcaaggg
     1861 tgcacccgac aagcagttta ccgatcctga cggagttacg cacaagctgt ggtgtgtaag
     1921 cgatgagaac tttatcgcaa ggcttaccgc caagatggct gataaaaagc tttacattgc
     1981 agacggtcac caccgttacg aaacggcact ccgttacaag aaattcgttg ccgaaaacaa
     2041 gcaggacgcg ggtacttccg aatacgttac catgatgctt gtaaatatgg agaacagcgg
     2101 acttgtgg
//
LOCUS       DAWAOG010000373         2100 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6896_length_2100_cov_2.505134, whole genome shotgun sequence.
ACCESSION   DAWAOG010000373 DAWAOG010000000
VERSION     DAWAOG010000373.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6896_length_2100_cov_2.505134"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          665 a          428 c          529 g          478 t
ORIGIN      
        1 taatgcacag gtagtcggaa agttcaatac taccccttat gataagctga agcttgatct
       61 tatgtatatc gaaaagtata cacttgccaa ggatatcctg cttatcctta aaacagtcaa
      121 gattatcttt atgcccgaag aaagtacaga gggtataaag gaaggatata taactcccct
      181 gcaaaaagga gaggacgtgc agaacaagga aggtaacaac aatggaaaca ttcagtaatc
      241 ttattgatct tgacgcatat tcacccgatg agtggaatat gatagttgag cttgcccggc
      301 aaattcagaa gaatccggca ctttaccgtt caagatgcag cggaaggata atgggaactc
      361 tgttttatga gccgtctaca agaacgcaga tgagctttca gactgcgatg ctgaggctcg
      421 gcggtacgat aataggattt gacaacccgt caacatcttc tgtttctaaa ggtgagaata
      481 tcaaggatac gacaaaaata gtaagcagct atgcggatat actcgtaata agaaatccgt
      541 atgagggcag tgcaaaggcg gcggctttaa ctgccgattg tccggtcata aatgccggtg
      601 acggcggaca tctgcacccg acgcagacgc ttacggatat gctgacactc aaaaacgaaa
      661 agggcagact ggatcacctg aagataggtc tttgcggtga tctgcgttat ggaagaacgg
      721 ttcattcaca gcttaaagcg ctgtcacgct atgagggtaa cgagtttatc ctgatatcga
      781 ctccggagct tgcactgccg tcatatataa aggacgtgct taatgcaaga ggctgtacat
      841 ataaggagat ctactcgctt gatgaagcta tagccgaaga atcgctcgat atgctgtata
      901 tgacgagaat acagaaggag cgctttgcaa gcgaggaaga atacaataag cagaaggacg
      961 tattccgcct tgataccgaa aagatgaaga aagcgccgaa ggatatgata gtgctgcacc
     1021 cgcttcccag agtggacgag atagcaatgg acgttgatga tgatccgcgg gctaagtact
     1081 tcacacaggc aaagaacgga atgttcgtgc gtatggcact tataatatca gtgcttgagg
     1141 gcagactggg cgctcctaaa ctgctcaccg gcacggtaaa gaatgatgtt gaatgcacaa
     1201 accccaagtg catcacaaag accgagcatt atctccccca cagcttcaag gtacagggcg
     1261 atatatgcga atgtgaatat tgtgacgagc gtattctgct cgactaagca agaggaaata
     1321 tatgatggat ttttcaacca aaaagagaat agttataaag gtaggcacaa gcacgcttgc
     1381 tcataacacg ggcaatctga atataagacg tgtgtcaaag cttattgaag taatgtcgga
     1441 tctgaaaaat tccggcaagg acgtgatatt tgttacatcg ggcgcacagg gcgtaggtgc
     1501 ggcaaaggca ggacttcact ctaagcctaa ggaaatgcct aaaaagcagg cttgcgccgc
     1561 tataggtcag tgcgagctta tgcacatata cgaccgtgaa tttgcaaatt acaaccacac
     1621 ggtggcacag gtgctcctta cgcgtgatgt tatcagcaac aagacaagaa aagagaacgt
     1681 tataaatacg ttcagcacac ttcttgatat gggaatagtc ccgataatca acgcaaatga
     1741 tacggtatcg atacaggagc ttgactttga tgagaacgat acattgtcgg cgatagttgc
     1801 ggcgttgtgt gatgcggatc tgctgataat actcacagat gttgacgggc tgtacgataa
     1861 aaatcccaag ctccccggcg caaagctgat tcctgaggta aagcagataa caagcgagat
     1921 gataagctcg gcaaaggaca agggcagtga gttcgcaagc ggcggtatgg taacaaagct
     1981 tgaagcggct actattgcaa aggaacacgg cataccgact gtaataataa acggtcagcg
     2041 tcccgaaata ctttacgatc tgtttgagaa taaggctgtc tgcactgtat tcaccgcaga
//
LOCUS       DAWAOG010000374         2092 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6948_length_2092_cov_1.830142, whole genome shotgun sequence.
ACCESSION   DAWAOG010000374 DAWAOG010000000
VERSION     DAWAOG010000374.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6948_length_2092_cov_1.830142"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1869..1878
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          536 a          508 c          493 g          545 t
ORIGIN      
        1 agcctgctac ttactgtatt ctaccgccat gagcatttat gcgacagcgg taagctgcgg
       61 ttacgatgat gaaaaattct tctcacggca gttcaggcag gtgaccggat tttctccggc
      121 agaatacaga aaaaagatac tgcggtgacg gcaagaatat tgttatgatc aataaacgac
      181 agaccgaggc gctgtaaaaa aataagactc ccatctcaaa aaacgagatg ggagtctttt
      241 tcgtggtatg atttacttga aatgaaaaat catccacaag aacattatcc accatatcag
      301 ctgagatttc cggtagaaat agggtaacac cgcacaaagc ccataaatta gaaattaagc
      361 aaatatataa tcactaccgc aagcattggg cattctggga agaaaacggt gttgaagtga
      421 taccgactgt atgctggagc gatgaaaagt cttttgaatg gtgctttgac ggtgagcctg
      481 tcggcggtac agtagcggta tcttctgtgg gtatgcagaa cagcaagacc gcaaaagcgg
      541 catttctgac aggttacgag gctatgtgcg acaggctcag gtccgaagca atattgtttt
      601 acggaaacgt gcctgaggaa tgtaaaggga atatcatgcg ggtaaagagg cataattgta
      661 cgatcataga cttgcacgac cgtagcgtta ttgcgagcgt cagcggaagt aatattacag
      721 ccgagcttgg aagccggact ctttccaagg caataagtca gcacccacag gtgctgaaaa
      781 acggcgttgt tctgcacagc gaccggggct ctcaatatac ttcaaaggaa ttcaccgact
      841 attgttcagc ttacaatgta acccagagca tgagccgtgc cggttgccct tatgacaacg
      901 ttccgatgga acgttacttc aacactttga aaagcgaact catttatcag cattcataca
      961 gttgcgagga taaattgtta tccgacattg agaattatgt cttactcctt ggcagaaacg
     1021 cttgacttga tcttttgggg aaggtgttac aattttgctt gacaagaaca atgaaaaagg
     1081 agcgaccggt tgcggaacgc cccttttctt aggcgacacc gcacaaaatc cggaaaatgg
     1141 tctttgtgtg gtgtcgctct tatatttctt atgtaactta ctttgccaga acaacagaca
     1201 gctttatttc tctctcggct tcgctaatgt cagccgcacc gccgtcctct ataactacct
     1261 gcacctcgta cagcttgtca gcagaaagct cggacggaac ttcgccgctc tcggtcttta
     1321 tgctccacag ctcatcggaa agctcggtca gcctgccgtc cgcaccgtat gcgtatagac
     1381 tgaattcatc cgcgttgcct gtcagaccgt tcatgcggat ggctacagta gtgctcgggt
     1441 gaacgttctc caccgtaata gtattcagcc agcccgaaac agtccccttc ccgccgtcgg
     1501 aaacgaactc ctcctgcgaa agcacgtttc tgttcgcatt gtcaaggtcg acctgcctgt
     1561 acggcatctc cggcagatat acaaatctgt cctgaagcct taaaagctcc agatatcccg
     1621 tggggcagta aagcgagttg ccgctgttcc ccgcgtacat tcctattgcc atgttgttgt
     1681 agccgtattt cgaagcaaca tccacgctga gaaccgtttc gcctgtttca aagtccagca
     1741 cgatgtactg ccacatccct gttttgagat cctggacata gccgtaaatg tatcccgtag
     1801 cggtcgaaag cttcatcata gatgtgtcac gcaggtcgtt tctgcaccat acgctcttca
     1861 tttcgtagnn nnnnnnnntg tcagtcttta tcgtgtccac gcgctcaaca cccggataca
     1921 gcatcttgtt gccctttgtg agccagttct tgtcgtagat gttttcgtag ctctgtatcg
     1981 aggagtcgtt gtcgggcttt gcaaggttgg gacttcctgc gccgaaccag ttgcatacta
     2041 tcgtgcttac tgtgccgcag ccgttgtcgt acactatcgc ggagttctcg at
//
LOCUS       DAWAOG010000375         2086 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6964_length_2086_cov_2.059084, whole genome shotgun sequence.
ACCESSION   DAWAOG010000375 DAWAOG010000000
VERSION     DAWAOG010000375.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6964_length_2086_cov_2.059084"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    248..257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    781..790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1205..1252
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          496 a          509 c          395 g          618 t
ORIGIN      
        1 atatacaacg gtatatcggc tgttttggct acatcgctcc accacgagca aacctcatca
       61 aatccaacaa cgtcatcatc aagcgaaccg gaagcatttg ccacgataaa gtccgccagt
      121 cctttttcga caaatgtctt ggtatcggca taaccgttat aatatgccga atatttagcg
      181 gaggtatcgc ttcctttttc gtttcttgat gaattagccc aaacgtcttt agcatccaga
      241 cctaccgnnn nnnnnnnaat aacctcactg actgtagcga atttgcattc gacactgtca
      301 aatagccagt ttttatagcc tatccccgaa cccttcttca tgtattcgtc atatacggcg
      361 ttattgtttg aaccgtaaaa tcctgtaaga attattccgt tgcatctgta cctgagagca
      421 aatttatgcg cttcactcac cagatagtca tacttgtcct cgccttccgg acaagctgac
      481 attgctctgt tgacatcgaa atacacaaac ctctgcattc ccttttttgt tgcgacagcg
      541 agtgcttttt caagcctgtc gccgtctttg ttcatatttg ttgtgaaata cgaaactttc
      601 ttatcataag gatttatgta tactccgttc attccgtagc ttataagtgt atccagctta
      661 gcggaaaaat tttcgctgaa cttctcagct tcaaaatcgc caagatttac tactgtagcc
      721 ttcagctcat ccgacaatgt aaacttcggc tcggcggttt gcggcttcgc ctgctgtgac
      781 nnnnnnnnnn ccatcacgct catttgtgaa agcagcatta tcacagacaa agcgcctgca
      841 attattccga ctgttctttt tctcatttat tgctcttcct tccgtgttga ctgtcactgt
      901 gtaaggagcg gcttgaacgc cgctatttcg ttttcaacag cagttcctac actgctgtcg
      961 ggttcaaata tgaacttata gctataaagt atgacaccgc cgtagtttgc ggtattctga
     1021 gacatttcta tctgtcgttt aatgatctgc gtattgttta tccattcgta tctgccgtcg
     1081 cctgcccatt cgtcctcagt gcctattttg tacactgcaa gaccgggtat aagcgttttg
     1141 cctgtacctg caagcatttc gtcccactgt tcaacgactt gctcaaacgg ctgaccgcta
     1201 ttatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgccatg
     1261 taatcaacat aaccgctttc ctcagaccat ttcttcacat ccgcataaag ctgtgtttcg
     1321 ttgttagtta catttccctg tgcgctgact ccgaacaatg cagtcggatt gtgtgacttc
     1381 accgcgttgt acatacccgc taccatgcgg ctgcaattat ccagcctgaa ctgcgacaga
     1441 ctgctgtacg aagaagcgcc aaaagcgatg ctgtcaaatg tttcatcggt tgtagggtaa
     1501 aaataatcgt ctatatgaac accgtctaca tcatatctcg aaacaagctc tgccgctccg
     1561 tttgcaataa ggtctgttac ctccttatac gcagggttga gccaccaata tgagtttact
     1621 ttgacaatgt agtcgccgtc tttggtatca taccactgac ctgtcttata tgatgtgctg
     1681 acataaggag catcatcttc ctgataacaa cgcaacgggt tgacccacgc ctgaaatgat
     1741 atacctcttg cgtgagcttc ttctatcatt atgttgagcg gatcgtagcc gggatcttct
     1801 ccgatatagc ctgtgcagta tttcgaccat ggaaaatagt cggatttata tattgcatcg
     1861 ccgaaagggc ggacatgaac atagaccgta ttgatgccaa gcgaaagaca gttatcataa
     1921 tactcaccta taccgcttct gagctgtgac tcgctttttc cggtaagaat cgggtacagt
     1981 tcggaatacc atatccatac acccttcacc tcgttataat tcagcgccgt gtagctgttt
     2041 acaggcagac tgtctgtaga tgaaggaacg ctgtcgctgt caggct
//
LOCUS       DAWAOG010000376         2085 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6969_length_2085_cov_2.096059, whole genome shotgun sequence.
ACCESSION   DAWAOG010000376 DAWAOG010000000
VERSION     DAWAOG010000376.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6969_length_2085_cov_2.096059"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1400..1409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          465 a          534 c          490 g          586 t
ORIGIN      
        1 cgaccacgtt ccgcacgaca ggaaagcaaa gcccttttcg cttgcgggta ctgctaccat
       61 ggcgctctgc gtatcgtgac cgcaggcggc gattatcttt gcgggcttta tatcaagctc
      121 cttgcagata tcatcggaca gtgtgccgac taccgttccg ccggttacga ttttcggcag
      181 aagcgaatgc tttatcttaa gacgttcaca gcattcttcc gaccacgttt tcgtatttgc
      241 gtcaaaaagc tgtgtggttg acgctatcga catttccgca acgggctttg cgccgagata
      301 ataagcgaaa agatcgggca tcataagcat cttgtcggct ctgtcgagaa gctccgcacg
      361 atcccttatt atgctcagaa gctggaacgc cgtgtttatt tccattatct gaatgcctgt
      421 cttttcgtac agcctgttat ggtctataaa acgcttgctg tagtccgaag tgcctgatgt
      481 tctgctgtca cggtaatgca ccgggttttc aagcagtctg ccggatttgt cgatcagacc
      541 gaagtcaacg ccccatgtat caatcgcgat gctgtctatc tcgccgtatt cctttgattt
      601 tataagtgac tgctttatct catggaacag acggagaaaa tcccagtaca ttgttttccc
      661 gagtattaca gggtcgtttg aaaagcggtg tatctctgtg agttttatta cagtgccgtc
      721 aaaagagccg ataacggctc ttccgcttga cgcaccaaag tcaatggcaa gtacgttttt
      781 catactgccg ccttatctta tgttgcggta cataggaccg taagcgttgc aagctctgaa
      841 gtcctgacct tccttatcca taccgaatgc attccaagcg gcaggacgga atatatcctt
      901 ttcgggtacg ttgtgcatag caacgggtat tctcagcata gagcatagcg tgataaggtc
      961 tgcgcctatg tgaccgtagc ttatagcacc gtggtttgcg ccccagttgt tcattacatc
     1021 gtaggctgtc ttgaatgcgc cctcacctgt tgttctcgga gcgaaccatg tgcagggcca
     1081 tgtgtagtct gtgcgcttcc acagcttgtc ggtaacctca tcgggaagat ggaccgtcca
     1141 gccctctgcg atctgtaata caggaccaag accctttacg aggttaagtc ttatcattgt
     1201 gcagggcatt tcagcttctg ttacaaatct tgatgaatat ccgccgcctc tgaaatagcc
     1261 gagatcggca tagttccatg tagtagcgtc aagaatattg tcaatatcct tgtctgtaac
     1321 gtcataccac ggcttcataa cgccgttgcc gttttcgtct gtagccttac cgcaggcatc
     1381 aaggcaggca gcacctgagn nnnnnnnnnt gtacgcacat cggcgaatat ctgagcacgg
     1441 tttgtaagaa gcttcataaa cagcattcca agaccgttga gtacatcgtt ttcggtagcg
     1501 agaacatacg gctctcttgc accgttccag tcaaacgatg tgttcagcat agcctcgggg
     1561 aagtcgcagt tggggtagaa gtcggtccac tgacgctgac cctggaagcc tgcggcgata
     1621 gcgttatgac ctacagcctc ttcctcacag cctgcgggga gatttttgtt gccgttcatc
     1681 agatccttta taatgcacat catcttaacg acaaattccc agtcctgttc cttctgctcg
     1741 tctgtgcggc ggaactgctc ggggttcttg tcaaagcctt ccttgcactt ttcacgggtc
     1801 catttaagtg ccttctgata ttcggcttca tcataaatac cctctgtcat tcggcggatt
     1861 atttctacct catcgacaga ttcaacacgc ataccgagat attcttcaat aaatgcgggg
     1921 tcgatgatag agccgccgat acccattgtg atagagccta tctgtaagta tgacttgcct
     1981 ctcattgtag cgcaggcaac agctgctctg ccgaaacgca ggagcttttc ctttacgtcc
     2041 tcagggatag atgtgtcgtc tgcctcgcat acatcgtgac cgtag
//
LOCUS       DAWAOG010000377         2085 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_6968_length_2085_cov_2.208867, whole genome shotgun sequence.
ACCESSION   DAWAOG010000377 DAWAOG010000000
VERSION     DAWAOG010000377.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6968_length_2085_cov_2.208867"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          484 a          589 c          412 g          600 t
ORIGIN      
        1 gccgtatgcg aactcaagcc ccgtaagctc gttttcaagt gccaccgtac agacagccgc
       61 aagcgacgcg cccgcattgc actcgataat atcgcccttt acccttaccg aaccgaaatc
      121 actgccgata agcaatacag ctccgtgaag tccctcgtcc gacacaagaa tattgcttcc
      181 tctgccgaca acatagtacc tgatgtcatt ctcacggcac agctttataa gctcgcacaa
      241 cagtgcctca cagtttatct ttatcatcac atcacacggt ccgcctatgc gaaacgaggt
      301 gtgagtcttc ataggttcat ttctgagcac gaccgcgccg tattttccgg cagtctgctc
      361 caatatgctg taatcgttca aagcatactc cttgcaaatt tattttttgc gatttttgcc
      421 aaataaactt tcctttatac agcttgtaga aaaagtctgc ttttagaaaa aatagagcaa
      481 attctctatc cctaacccca aaccccttcc ccttgggaag ggaccgaatg tggggctgcc
      541 gccctcaccc cgcttggggc tacgccccaa accccatttt caaaaattat ataggttttt
      601 cgacaaactt ccccttatga gcctttaatc cttgagtcta tagagattcg ccgcaccgac
      661 tattcccgca tcatttccga gcttgcatat accgaggacg gttgaacgct ccggatttat
      721 gcagtagctc tcgcgcttga tatactcttc aagcggcttt gtaaggtaat cgccctcctt
      781 gcagatacca ccgccgataa gcagcatttc aggctggaat gtatttacaa catttgtaag
      841 tccgcagccg agataattca gatactcgtc aactacagcc ttaccgctct tgtcgcccgc
      901 tctcatagcg tcaaacgccg tcttgccgtc aaccttgtca aggctgcccg caatgctcca
      961 cattgtactg tccttatcct cttccatagc cttctttgtg gctgttataa gtgcagtagc
     1021 cgagctgtac gcctcaaaac agcccttcct gccgcagttg cactgtctgc cgttgtattc
     1081 aattacaaca tgaccgagct ccgcgccgca gaagttgaag cctgtgtata tcctgccgcc
     1141 aatgatgata ccgccgccga cacccgtgcc gagcgtgatt acgacaacgt ctttatatcc
     1201 ccttgccgca ccgccgagaa cctcaccgta tgccgcaaca ttagcgtcat tttcaacata
     1261 aatatcagta ccgaggcgtt ctctgagcag tccgccgagg tttgtgttat tgaagccgag
     1321 gttgcacgaa taaagcacaa ctcctgtgtt tctgtccgca acaccgggag taccgatacc
     1381 gatactgttg atatccttta caccgatacc cgcttcctta caggcaagct caacaccctt
     1441 tgctatgctg tccgcaattt cttctgcggg acgcggcagt gccgtcttga tattcgactt
     1501 gcctacgatc ttaaaatcat cgtctactac tccgaccttt atgtttgttc cgccaaggtc
     1561 aattcctatg ttgtacatta tgttatattt ccttccgata tttaatatat tccttgcttt
     1621 ttatgcttat tcgccgtcag tcttcatcag gctcgatttc agcgtttgtc tttatcccct
     1681 tgcttttaag gaaattttca aagcgttcaa gatccgcatt gactatcagc tcttcgggga
     1741 aaccgatatc ctttaatagc gcctctgcct ttttgaacgc gcctatttta ctgcaaaaat
     1801 gtgcatcgga gttgaccatc acataacagc cgttctttgc acaggtcggc aatatgatat
     1861 cgtgcaggcg ctgtatagag gtctgacttc tgaaacggct tacgttgaat tccatggcaa
     1921 cgttcttttc tctgcacgcc tttgtcaccg cgtcatagtc atacggataa tccgcagttt
     1981 cggtgtgtcc gagaacgcac accttcgggt tatccataac ctttaaatat gcgtttgtat
     2041 gttgctccgc agtgccgggt gttatgcaag gattatgcat tgatg
//
LOCUS       DAWAOG010000378         2034 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7056_length_2064_cov_2.034843, whole genome shotgun sequence.
ACCESSION   DAWAOG010000378 DAWAOG010000000
VERSION     DAWAOG010000378.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7056_length_2064_cov_2.034843"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    544..553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          554 a          500 c          460 g          510 t
ORIGIN      
        1 tatccgttca ttacggacct gcgggggcgg gagtagagct tgccggagtt gtttcgtccg
       61 ggggcagatc aacctgattg aacatagtgt aaccgagcat ggaatatctt tccggatagg
      121 cacctaaaag atagccgtct acataataat tgtcggtggg ttcgatcgtt gtctttgaaa
      181 aatcacttga tgtatccgtc actcggctta tatcttcaat aacggtttca caccaatatg
      241 tcattactac cttgcactta tcatcgacca gggcaaaata atacccctcg tcctcgtctg
      301 ttgaaaatgt gttcagctta tcaagcagct cgtgatccgg acttgcggaa tctatcgtta
      361 ccgcatactg aaactgcaaa tatccctgtg ccgtattggc agggctgtca tcggagggtg
      421 cgtcaagctt tgcaagctta agctctgtga agcccttata tttctctgcc ttggcgcaca
      481 tataaagata gtcgcccgag cctacggtat aagtcattcc cccgttttcg taagacagat
      541 agcnnnnnnn nnncagatag cctgtctgct tttcgggcgc atcggctctg tactgtataa
      601 gtatcctctg tgagccgaaa tagaactcct tagcctttgc gacagccgcc tgaggctttc
      661 ccgcattcag cgaaagcggt aatattatag ccgtcagaac acctatcatg gcaagaacaa
      721 cgataagctc aacgagcgta aaaccttttt tattatgaag acgtatcaat ctacgcatct
      781 gcctgacctc cccttaaagc cgtggactat aactcaagat aattttacca tattttttat
      841 gcaatgtcaa ctgaattttt atactttgcg agggtgtttt tagagttttt acagacagat
      901 tgaggggagt gggtagcgta tgctaaaccc cccgccgatc agcggcgggt gtcgttcaat
      961 cctcattatc acttacagtc gtatttttaa caggaaaccg tcttttgagt atcttcaccg
     1021 aatggaagaa cgcggggaag aggaacagat cggcaagcac ccacgccgcc gggcttgcaa
     1081 ggcatacggc tacataaccg aaagcgggca caagtcccaa tgccacgccg ccccttgcta
     1141 tcatttcaaa tacacccgca aatacggcta gctggctgtt gcccagtccc tgtatcgaaa
     1201 agcgtataat atttacgaat gcgagcggga tatagaacac cacgttgagc cacaaaaact
     1261 gctttgcaag atccagtatt tctgtctgcg ttccgtctat aaacagcttt gaaaattcgc
     1321 cgccgaacgg tattatcacg ataagagcga ttatcgcata ggctatgccg agtatcgagc
     1381 atttcttaac gccgctttcg atacggtcga ctcttcccgc gcctacattc tgaccgcagt
     1441 aggtagccat tgtagagccc attgcgtcaa acggacagca caggaactgc gtcaccttgc
     1501 ttcccgcagt taccgccgcc atataggttt caccgagcga atttaccgcc gactgtaaaa
     1561 gtatacttcc gatggcggta atggaatact gaagtcccat aggcacaccc atagcgcaaa
     1621 gtctgctcat tgaataagcg tcgggtttta aatctttcct cgataagtgc agaatatcaa
     1681 agcgctttat catgtagaca aggcaggcaa taccggatat aagctgtgca aggacggtag
     1741 cccagcctgc accggcaacg cccatatcaa acacgagaat gaacagcagg tcaagtccga
     1801 cattcagaac acttgaaatt atcagaatga caacgggtga ctttgaatcg ccgagcgagc
     1861 ggataaagcc cgataaaatg ttatagagga acgttaccgg gatacctaag aaaatcacga
     1921 aaatatagtc atacgcttct ttaaaaaccg tatcgggcgt attcatccaa tggaggatat
     1981 ttccgcaaag aaggcaggta gcggttgtca tcactgcggc gaagccgata ccga
//
LOCUS       DAWAOG010000379         2061 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7068_length_2061_cov_1.777168, whole genome shotgun sequence.
ACCESSION   DAWAOG010000379 DAWAOG010000000
VERSION     DAWAOG010000379.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7068_length_2061_cov_1.777168"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          588 a          424 c          498 g          551 t
ORIGIN      
        1 ggaggaatgg cagctgagac ggtcggtttt tcgggaaatt gggagccttt cggcgctgac
       61 ctgtctccag cagtcggtga tgaacttcgg catacttatg atacaaggtc ttgtaaacag
      121 cttcggctct gcggtaatgg cggcgtttgc ggttgctgtg aagattgata ctatagccta
      181 tatgcccgta caggacttcg gcaatgcatt ttcggttttc actgcgcaga acttcggcgc
      241 cgggaagcac gacagaatca aaagcggaat aaagcaatca ctgataagcg ttgtactgtt
      301 ttgtgccgct gtaagcacgg ctgtattttt cctgtcagag tctcttatga gcctgttctg
      361 caccgacagc agcactatag caataggcac acagtacctg agaaccgagg gtctgtgcta
      421 tgtcggcatc gggcttctct ttatgctcta cggttactac agagcggtga acaagccgct
      481 tatgtcggta atcctgacag tcttctcgct cggaacaaga gtacttcttg cctatgcatt
      541 gtcacctgtg cctgcactcg gagttacggg gatatggata tctatcccta taggctggtt
      601 tattgccgat gcagtcggag tcggatatta catgatttgc ggaagaaata caatatatta
      661 cgataaacgg tgaaaaacgc gccgcaaaat tgtaaacaat ttcaaacttt acctattgac
      721 aaaatccgta tattatgata gaataatcaa tagatataca gtgaaactat taggacgatt
      781 ttcggaggtc agatgacgga aaagcttaat atttctgata tgaccgacga acagctcgtc
      841 gatctgatag tacaggagca cggtgaagaa tgtgccaacg agcttataaa acgctacata
      901 ccgatcttac gcgttaaagc ggcaaaaatg gcgttgcgct gtccgtcaac cgataaggac
      961 gacctctttt ccgagggact tatgggactg cttaaagctg tacggcttta taacggagaa
     1021 aaaggcgcgt catttgccac atttgcaaat ctgtgtgccg actcggctat gaagacctgt
     1081 atatcaaaag ctatcaagga taacccgata cttaaagacg atgactttga ctttgatctg
     1141 atacgcgatg attcgctttc caccgaagat gcggtaattg ataaagtcgg tgatattcag
     1201 tttatgaaac gtctgtccgg cgtactttca aaaaaagaga tgaaagtcct tgatatgtat
     1261 ctgaagcact gctcatatga gcagatagcg actgaactct cgctgaacga aaaatctgta
     1321 gataatgcac ttcaacgtgc aaaatcaaag atacgaaaag caatggggaa ataagacttt
     1381 cagtcttttt catattatta cacgcaaaaa gcgtgagaaa ttttagaggt attttactat
     1441 gtacgaagac aagactctca agtgcaaaga ctgtggcgca gattttgtat tcacagcagg
     1501 cgagcaggaa ttttacgctc agaacggttt cacaaacgag cctaagagat gcaaggcttg
     1561 ccgcgatgcc aagaagaacg gcggagctaa gcgtgaaatg ttcacagcag tatgcgcagg
     1621 atgcggcggt gaggcaaaag ttccttttga gccccgcgat gacagacctg tttactgcag
     1681 cgagtgcttt gcaaagagaa acgagcgctg aatttatcaa caatactaat cggttcgggt
     1741 tttaagtcag gctattcaga ggagtttcgt acatttttct atattcctga tcataatgcg
     1801 aatagttgca tatagtattc aaggtaaaag aagagagcct tgccgtgtgg caaggctctc
     1861 ggcttgtata aataggctgt ttttgagaaa tgcagcttat tctctatccc tttaagaact
     1921 gcgggctttg catcgtgcat tcgtggtcgt ttgttgtcgg tgcgtcactg ccacttcacc
     1981 actgactttg cagagatgca aaaaccggca gagcaagcaa cgctaaacac cccattttta
     2041 aaaattatgt aggtttatcg a
//
LOCUS       DAWAOG010000380         2056 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7085_length_2056_cov_2.105947, whole genome shotgun sequence.
ACCESSION   DAWAOG010000380 DAWAOG010000000
VERSION     DAWAOG010000380.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7085_length_2056_cov_2.105947"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1301..1310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          602 a          459 c          426 g          559 t
ORIGIN      
        1 ccgtacatga gcattaaacc gggggttgta tcatcttatt ttaaaattaa actaagattg
       61 agtgttcatc gttaggatcg aacaggtgta ccttgttagg atcaagagca agcttgataa
      121 cgtcctgagg acgagctgta catctgggag atactcttgc cgtcagcggg ataccttcgc
      181 atgtgaggta gaggtaagac tcagcaccca tcatttcggt aacatctact gtagcttcga
      241 tcatacctgt cttagcgtta gagaggaaca tttcttcatc gtgaatgttc tcgggacgga
      301 taccaaggat aacttccttg tcaacatagt acttgagagc ttctgtatct gtcttagcac
      361 cgggcagttc aacatagaac tttctgcctc ttgttgtctt tgtatcttca gaaccgaact
      421 caactgtgta cttgccgttg agcatgatga gcttagcgtt gatgaagttc atctgaggtg
      481 aacccatgaa gcctgcaacg aacttgttaa cagggttctc atacaggttg ataggtgaat
      541 cgatctgctg gatataacca tccttcataa caacgattct gtcacccatc gtcatagcct
      601 cgatctggtc atgagtaacg tagatgaatg tagtacctaa cttcttgtga agcttagaaa
      661 gctcagttct catctgtgca cggagcttag catcgaggtt tgaaagaggc tcgtcaagta
      721 agaatacctg aggatcacga acgatagctc tacctaaagc tacacgctgt ctctgaccac
      781 ctgataaagc cttgggcttt ctgtcgagaa ggtgtgcgat gtcgaggatc ttagcagctt
      841 cttcaacgag cttcttgatc tcatccttgg gcacctttct cagctttaag ccgaatgcca
      901 tgttctcgaa aacagtcata tgaggataca gagcgtagtt ctggaaaacc atcgcaatat
      961 cacgatcctt aggagctacg tcattaacga gtctgtcgcc tatgaaaagc tcaccctcgg
     1021 aaatttcctc aagacctgcg atcattctta atgttgttga cttaccgcaa cctgaaggac
     1081 caacgaggat aatgaactcc ttgtccttga tgttcatagt aaagtcggaa acggctacta
     1141 caccgccggg gtaacgctta tagatacctc ttaaagataa acttgccatt tatgtgtgac
     1201 ctcctgtttt attgcagaat aaccttacgc taatatttta acaaaatctc accgaaaatg
     1261 atagtaccta attacccaaa tatcaaaccc ctgcgttatg nnnnnnnnnn aatttttagc
     1321 gttgaataaa gtaaaaacaa gctcattttg ttataaatca caaatgcagg agccgaaatt
     1381 tagtgaaatt ttgcaattag tgcttaacac agtgaacggt tgcgctgaaa attcggcatt
     1441 tcgatgaata ttttagcata aattttagta aatttcaacc ggtagtcccg acatttatcc
     1501 gacaaactaa aaacaaagcc gatggaggaa caatgaagaa ccgaagaaag cttatccgta
     1561 taacaataat acccgccgtg cttcttacag cattactgtt tacttttctg ctctgccgcc
     1621 gcccctcaat atgcggtgaa actttaaccg acaggacaga atacgcaaag tcgctcggat
     1681 atgccgtgtc acgcgacagc gaaaccgtcc gcagcatagt tatccccgcc gtattcggca
     1741 atgtctatga aaactataac gcattgcaaa aggaatgcgg ctttgacttg tcgctgtaca
     1801 agggcgaaac tgccgtgcag tatacatata ctataagcaa ttacacagac gacagcggaa
     1861 aaacgcttga taatattata atgaacctta tcgtctgtga cggaaaaata atcggcggtg
     1921 atatctgctc cgccgagctt gacggcttta tgaccgggct taaagcaaag aagtaggata
     1981 tacaaagaaa gattttgccg ctctgccgtc aaaacagagg gatcactacc accaacattt
     2041 tcattccaac catcac
//
LOCUS       DAWAOG010000381         2053 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7099_length_2053_cov_2.348348, whole genome shotgun sequence.
ACCESSION   DAWAOG010000381 DAWAOG010000000
VERSION     DAWAOG010000381.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7099_length_2053_cov_2.348348"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    865..874
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          456 a          544 c          428 g          615 t
ORIGIN      
        1 agtctactct tatcttatat tttctgccta ccgtcatctg taaaaggtaa gtcattaccg
       61 aagacgaaag accggtcgtg tagctgttaa gcactatgaa cagcggcttt tcggtcaaca
      121 cctcggtgca catattcata aggtcgcaga tactgtcctc gagcttccac atctctccgt
      181 ttgttcctct gccgtagctt ggtgggtcga gtatgatgcc gtcatagcgg tttccgcgcc
      241 ttatctccct gccgagaaat tttaccgcat catctacaat ccatcttata ggtttgtctg
      301 aaagaccgct gagctttgcg tttgtcctgc cccagtctac catgcccttt actgcgtcaa
      361 ggtgacatac ctttgcgcct gcttttgcac aggcaagcgt tgcgccgccg gtataggcga
      421 acatattaag cacgtttatc tccctgccgg cgttctttat aagctctgag cataaatccc
      481 agttgaccgc ctgctcgggg aatatcccgg tgtgcttgaa acctgtgggc tttaccataa
      541 aagtaagacc tttgtagcta atattccagc tttcggggag ctgttttttg tactcccatt
      601 tacctccgcc gctcgatgaa cggtgatata ccgcgtccgc cttgttccac agcggtgagg
      661 ggttcggatt tttccatatt atctgcgggt cgggtcgcac aagcgttata ccgccccact
      721 tttcaaggcg ctgaccgtct gatgcatcta tcagtctgta gtcctgccaa tcctgtgatt
      781 ttctcaaatt agttcacctt atcctgtaac tgtctaccgt cggtcgtatc gtgttgagaa
      841 acgagttctc tccgatcgta tttannnnnn nnnngaagaa cagagcctga cttccgcccg
      901 ttgaagttac cgaaacgaat attctgcctt ccgtctggaa cagtgtgacg ttcatactta
      961 ctctgccgct tccaagacag ctgtaacgtt catatgcacg gactgcgcaa cgtgtacttc
     1021 cgactacaat atcagagctt tcttcaagag atgcactgga actccctctc attacagtgt
     1081 tgtgaagatc cataagtata atatcgaaat tgccgtacag ctctgcttct atctttgcca
     1141 tttacaaact ccttttacat cagcacagac actcttttat cgtgtctgcg ataagatttg
     1201 tccagtgatg cacttccttt tcgtctttgc cctctatcat aactcttaca agcggttctg
     1261 ttccgctttc tctgacgagt atcctgccgt tttcacccat agcctgttca gcctgtgcga
     1321 taatatcggt tatcttctgc gtcttgtccc atagtccctt tttgtcctcg gtgatcttta
     1381 cgttgacaag aagctgggga aagtcgggta tgtcctttac aagctgtgac agcggacgct
     1441 tgcatttcga cagaagttca agggtctgcg ccgctgtcag ctgtccgtcg cctgtggttg
     1501 catggtcaag gaatataata tgtcccgatt gctcaccacc gatattatac ccactgttaa
     1561 gcatttcttc aagcacatag cggtcgccga ccttagtaca cacggtatta atcttatttt
     1621 cgttcataaa acggtgaaat ccgagattgc tcataacggt gactaccgcc gcgttgtttt
     1681 taagcttacc catttccttc atatagcgcg aaaggatagc aataagcttg tcaccgtcta
     1741 ttatattgcc tctttcgtca acggcaaggc atctgtccgc atcgccgtca aaagcaaagc
     1801 ccacgtcaca accgcgcttt ttaacaagct cgacaagctg ttccatatgg gtagaaccac
     1861 agttatcgtt tatgttaaga ccgtcaggct cgttgtttat atacacatat tcagcaccga
     1921 acttatagaa cagcgattcg gctgttgcac tggcactgcc gtttgcacag tctattgcaa
     1981 cgagaagccc ctgaaacttt ccgccgacag tcgactgaat atgctcgttg tactcgctta
     2041 cagcgccgta cat
//
LOCUS       DAWAOG010000382         2052 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7106_length_2052_cov_1.877817, whole genome shotgun sequence.
ACCESSION   DAWAOG010000382 DAWAOG010000000
VERSION     DAWAOG010000382.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7106_length_2052_cov_1.877817"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          505 a          541 c          383 g          623 t
ORIGIN      
        1 cgtacaaagg tacgtcgagg ggcggttgac acagtaagca aaaatgcttt caaacagccg
       61 ttttttacgg ctgtttgaaa gcatttttac ttatccgatt tttagtttat ctaaaacttt
      121 ttatctgcat ttttgcggtg cgtgggttta tcgacagtct gggctccctc acaaacagtg
      181 agggagcttt tctgtcaccc gtcacgcacc gcctatcccc tgcgaaaaaa tatcaatatt
      241 cacattgacc tcaatttcac tttccgctat tatttcccct accctgtcca tattctctct
      301 gtaaaatctg ttacagcacc ttcttagcgt attttcaagc ccgaacacat cacagccttt
      361 ttctgcaatc ggtatcaccg agctttctat tttccgtttc agttcccttt cggcaggctc
      421 ggctaccttt tcggcggctt ttttatcatc ttccttcatt ccgcccttcg tccctatctt
      481 tcccgatgcg ctgaatgtca catcaaattt caccttacca ttatattcag cagcttttat
      541 actccttgta acttcaataa gcgtaacgct cggctttgcg ttttgtccca aaggcacggt
      601 aaacgaatat tccgaaggca tatcattcat aaagaatata gccattgccc catcatcgtc
      661 aactctgcaa agctctttac cgcccttgaa aaccactccg ccgtcaagta ttatcgtctt
      721 ttctccgtcc tcgctctgtt cttctccgcc tccgttgccc ttaccgcctc ctgtttcctt
      781 cggaatagtt acatctgtct taagtgttgc ggttctgagc agcggaagaa atgcgctctc
      841 tgtagatgtg catagcatag tcatcacctg gtagggatat gtgcttttgt tcctgctttc
      901 gaccgatgcg ttgcgtataa ggttcgccaa tctctgcgaa gaggtcgagc cttccttgaa
      961 gcgtatatcg agcagttcct ctgccgtatc ctctgcggcg gcaacatatg cctgcggatg
     1021 cgaatggtaa ttgcccacga agaattcaag cgtgtcgccc agtttatact tcctcagttc
     1081 ttttccgatg attatcagcc tgttttcgct cagcagaaga tcctttcccg tcagcatcga
     1141 agcgtttttc gccgccgcaa atatgctttc gccctcccct acgcttttca gcacattgtc
     1201 ctgcgtcttg tctatctgat tctgtccgcc ctcgctcgat tgattgaaat actgcatact
     1261 tatacgatat acctttttgt caggaagata gtctatccct atcgcctgca ctataactct
     1321 gtccgcaaga tcgatgtaat ttgcgcagcc gcacagaccg agtgttaata aagtcgatac
     1381 aagcactgcc gcagtcctaa atattttcgt cattcttttt taccctcctc tttgctttgt
     1441 tctgcctgct atcaggacga caagcggcac aactacgccg ccgatcaata tgtgtatccc
     1501 gctgttcatg attttggtca ttctttcata aacgccggca tcactgccta aaatcacaca
     1561 gagcataaaa atcaaaaaac cggatactgc gaccgcaagc gttgtatgcg gcttattact
     1621 gcacaccgta tagatcctgc ctatgcagta aataagcagt gcggctttta ttataccgca
     1681 cataagccag actatggcat ctataccgtc aaggcgctga aacagcacga tatcggacaa
     1741 tgacgcaatg atataaagcg gatattccac ctttgtcata taaggaccga gtatcaggtt
     1801 ataaagcata ttgagaaatt ccatcaatat caaaagaatc ggcaggtaat aaattacggt
     1861 cttgtacggt ttactgcgca gttcatcggt aattacggcg aatatcaata tctcgctgtt
     1921 catacttatc tcggagatca gggttttcag aaaatgcgac cggtcattta taaaggaaaa
     1981 atacagataa tcaggctcga ccttatccca catagtaaca cctataagaa atatcaacag
     2041 accgaatacg gc
//
LOCUS       DAWAOG010000383         2050 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7118_length_2050_cov_1.821053, whole genome shotgun sequence.
ACCESSION   DAWAOG010000383 DAWAOG010000000
VERSION     DAWAOG010000383.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7118_length_2050_cov_1.821053"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1326..1335
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          683 a          440 c          458 g          459 t
ORIGIN      
        1 aagatatttc aaaagaattt gagaaaattt cggagggcgt tgaaaaagca cgcaaaaaac
       61 gtaaatcact gacaggaaag atattcaagt ggatgtgcct tgccgtaggc ggatcacttc
      121 ttatagtcgg cggaataaat atagggatga gctataactt tcttaaacag tcactgaaag
      181 accagctgac agaggttgcc gatatgtctg caaaaactat atcgaaccag ctgagtacga
      241 tcactgctga acttcagcaa atatcatatg actccggttt tgaggatctt tcggatacag
      301 gtacgctttc tacaaagtgc ttttcgatac ttacgaaaaa cccgatgctc accgatatca
      361 agatagtcaa tacagacggc aagtgctttt atgccgaaac gcttgattat tccgaaaacg
      421 aaagcttcaa gcaagctgtc gaaaccaaaa aatccgcaag cggcgaaccc tatgcggcaa
      481 atgacctaaa acacgttctc atggacattg cagtacctat gtttgaatcg gataacagta
      541 cgctcagaag tgttcttatg gcaagcgttg acatgagcac gttctctgca gtgatcggtc
      601 agacaaagat aggtgaaaca ggctatacaa tggctgttga tcaaacagga actattatcg
      661 catatcccga tgaaactaag cttacagagc gaataaacta caagaccctt gctcaggcgg
      721 acagctcgta tcagggcatt gccgattgca tcagcaaggc tatcagaggt gaaaacggct
      781 ttgcggaaat aaatctggac ggtgtcagca aatacgttac atatgcacct attgagaata
      841 ctgccggatg gagctgtata gtagtagcta ctccctccga atataccgga agcatttttc
      901 agtcagtagt aatcggctct gtagctgcag taatctgttt tattatttca gtactcctta
      961 tactcacaat agtcaagaag ataatcaagc ctgtaaagct ctgttctgac agaattgtta
     1021 cgctgtcaca gggtgacctg cactctgctc cgcttgattt tggtgacaag atcgacaagg
     1081 agataggtca gctcagtgaa agcacaaacc ttataacaac gcatataaat gcagtaatat
     1141 cagatcttga cagtatgctc gccgcactcg gaaacgggga tcttacatat tcacccgccg
     1201 atgtttacct tggtgacttt gctaagctca aagcttcata tgaacgcata atggcatcgc
     1261 tcaacaagac gatgagcggc ataagcatag caggcttaca ggttgctaac ggagcgggac
     1321 aagtannnnn nnnnngcttg aagctatgca caagatcgat gatacatcaa atcagatcgc
     1381 caatatcatc aaaactatcg atgatatctc attccagaca aatatactcg cgctcaatgc
     1441 ggcagtcgaa gcggcaagag caggcgaagc cggcaagggc tttgctgtag tagccgatga
     1501 agtaagaaat cttgcaggca aggttgcaca ggcggcgagt gatacaacag agctcatcag
     1561 cgattctatc aaggctgtcg aagacggtac tgcaatagca aacgaaaccg cgcagacatt
     1621 gagccttata gtagaaacca ccacgcagac tactgcattg atagccgata tttcaacagc
     1681 ctgcgaagaa caggcaaacg ctatatcgca gatatcgaca ggcgtcaata cttgatggca
     1741 tggtaagcaa gttcaagctc gacaataatg ttttaaataa taaagttgac gcagaaacgc
     1801 ctgcgcccga cattgcctca gaagcaaaac cgattataac ggatctcggc gaaaaagaaa
     1861 aagctaaggc tgatatcggc aagcataacg aaaaatctgc agcaggcatt aaaacaaata
     1921 aagaaccgat ccggaaagac aaaacagccg aattatcatc agttcagata aagccggtcg
     1981 aaagcgaaaa gcctgcagtg ccggaaaaga aatcaaaaaa cgatgaaaat gtgaaaactt
     2041 ccgtaaagga
//
LOCUS       DAWAOG010000384         2046 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7139_length_2046_cov_2.192868, whole genome shotgun sequence.
ACCESSION   DAWAOG010000384 DAWAOG010000000
VERSION     DAWAOG010000384.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7139_length_2046_cov_2.192868"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          467 a          540 c          402 g          637 t
ORIGIN      
        1 gctatatcgc tttcactcaa atcgggcaac agcttttcac aaacgtgcct aaaaccgttt
       61 tcgtaataat cgatccatac gcccggcata cctttatatt ccataagcgt accgcctatg
      121 tcaaatgcgg ctaccttgat catatctgcc tccgatagta gttattcaag ctcagctaca
      181 gttacgcccg cttcaccctc gccgtaagcg ccgtcgcgga acgacttgac gtgcggattt
      241 ttccgcagat actgctgaac tgccgcacgc agagcgcctg tacccttacc gtgaattatt
      301 gtaataaccg atattccgct taaaagacag ctgtcgataa agcggtcgac ctccataacg
      361 ccgtcatcac cgagcatacc tcttatatca agctccatac cgacctttga tggggtaggc
      421 tgtttcttgc tttccttttt ctcttccaca aggcgcagat tttcaagctt ggtcttggtt
      481 ttcatagccc cgacctgaac aaagcacata ttctttgaat cgggcaatct gagtaacgtt
      541 ccctctttgt tgaggtctgc aagtctgacc gtatcgccga ttttaagcgg tctgggaagt
      601 acataatgat ctactttctt ttgcattata gggtttgctg tgtcatacat tccgttcaca
      661 gatgagttga tatgtgaacg cgcacctttg accttctgag caaaatcagc actttctttc
      721 tgctttctga gcctttcaag ctcttcaagc ataaggtcgc cctgaaaacg cacctcttcg
      781 ataatgcttg ccgccttgct tcttacgtcc tgaagctctt tttccttgtc tttttcaagc
      841 tgatcacgtt ctgctctgag ctgatccgtg atctcctttg actttctttg ctctgccgcc
      901 gccgagcttt tcagcttttc aagctcctgt cttgtctttt caagcgactg tacaacctct
      961 tcaaagcgct tgtcctctgt acttacaagc tcctttgcgt tgtcgataat atcactgccg
     1021 ataccgagct ttgaagatat cgcaaaagcg tttgacttac ccggcatacc gacaataact
     1081 ctgtatgtcg gtcttaacgt ctttatatca aactcacatg aagcattctc aacgctgtct
     1141 gtctttatag catacatctt tacttcctga tagtgagtag ttgcgataac cttgcagtca
     1201 cgctttctga actcatcaag gatagataca gcaagtgcag atccctcaac agggtctgtg
     1261 cccgagcata gctcatcaag cataataagc gacttatcat cggctgttct cagtatgcgc
     1321 gcgatatttg taatatgtga tgagaatgtt gacaagctct gctcgatgct ctgctcatcg
     1381 cctatatcaa catacagctc atcaaacata cctataacac tgttctcgct tgcaggtatc
     1441 atcataccgc acatcgccat aagcacaagc agacctgcgg ttttgagcga aaccgtctta
     1501 ccgcctgtat tcggacctgt cactataagc gatgaatatt cattgccgag ttcaagcgat
     1561 atcggcacga ccttatcctt atcgataagc gggtgtcttg cctttatcag gttaaagcac
     1621 ggcttgtcgg taattacagg cgttacggct ttcatcttag cgccgaggtt tgctttggca
     1681 aagtaaattt cgagcgtcag gactatctcg taatcgttta tcatcggttc tgcgaagctg
     1741 ccgacaagct cagacatttc ctttatgata cgctctatct ctttctgctc ttctatctgc
     1801 aatactctta tctcgttatt ggcttcgact accgccatag gctctataaa cagtgtcgca
     1861 cccgttgcgg acgtatcgtg aacaagaccg cttatttcgc ttttgtactc ggtttttaca
     1921 ggtacaacaa atctaccgtc gcgcattgtg acaaggcttt cctgtaagta tttctgcgta
     1981 gattggctct ttatcagctt gtcaagtctt tctcttaccg caaggctctt gcgctgtatt
     2041 gagcgg
//
LOCUS       DAWAOG010000385         2045 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7149_length_2045_cov_1.854271, whole genome shotgun sequence.
ACCESSION   DAWAOG010000385 DAWAOG010000000
VERSION     DAWAOG010000385.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7149_length_2045_cov_1.854271"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          494 a          577 c          334 g          640 t
ORIGIN      
        1 catattcgca gattcaacgg gtctgccgtc atcgctcacc gcaacacagc ctgccgcctt
       61 aagctcgtca tagtcgcaca gttccttgcc ctgcataccc tttgtgatac agccgacagg
      121 ataaactttc gcctttgctt tctttgcctt gtcgagaata tattttaccg tttccttgtt
      181 gtcaacgggc ggtttcgtgt tcggcataca cgcaagcgcc gtaacaccac ctgccacagc
      241 cgcctcacag cctgtaataa tatcctcttt atatgtctgt ccgggatctc tcagatgaac
      301 gtgcatatcg catatacccg gtattaccgt aagcccgttg cagtctacca cccttgcgtt
      361 cttatcatcg cattcaaggt tatcgccgag cgcacggata aagccgtcct ttataagtat
      421 gtcctttttg ccttcaaaac cgttcacata atcaacaacg tgaccgtttt tcagtaaaat
      481 tgtcattaca gcacctcgca attctgcaca aaagccgtgt ctttcttcct tgacgatgaa
      541 aaacacggct ttaattcata tccgccctta gcagataaaa gatcccattt tacgaaagat
      601 atgctaatat ctgtatcgca caccctcgcc tatttccata gtcattttat tatactatat
      661 tttctgccgt ttgtctacaa gtttacgaaa tttttttaat tttttatttc acttaccggt
      721 actttatctg cgtcctcgtt tctgagtgca attaccgcac ctcttacaag atatgccttt
      781 atcccgctac cgcacaacac gcaggtgact gttgttcctt ttgtaaagcc gagatccata
      841 agccttcttt tcatatctcc tgcaagtaac agcctttcta ccctgcaatt ttcattcatt
      901 tcaagctgtg ccagacttct cacataaacg cctccttcat ttcctcgctt atcgccagtt
      961 tatgaaagaa agccgcagac ggttaaaccg catttttgcc caagcgttac gcaaaaatcc
     1021 tttttcgaga tctccgtatg ataacaacgt ttttacacct tgctaaacct caccgtctta
     1081 ttcttcatcg atattttctt tacagcatac ccatagccgc tcagttcttt cttatacgtc
     1141 agaaaggaat gatctatcgg cacaccgcat atcttctcta tctcttcgta agtcagcata
     1201 atttcaggct cttcgcacta cccgatatac tcccacaaag aatcgtactt gctaatatat
     1261 cttcttacaa aagcctcatc atcttgcatt gctcaagccc tgcgctccaa aactcagaca
     1321 gcggcagatt ctttatccgg cacacaatgc ttgcattcat tgcaccgtgt ccgactatca
     1381 gaatatcctt gccctctttt atctgcggat agattacctc gtcaagaaat tcacccgttc
     1441 tgccaaacag ctcgtcaaat gtttccgccc cgcctataga gcttgtatac tgctcggggt
     1501 ggaaaaacag taagtttatc ggacagtcgg gaatactgaa gctgttctcg ataccctcat
     1561 attccccgaa gcacatttcc ttcagcctgt cgtccgttac tatcggtaca tttctgccct
     1621 cgagcaatat ctcggctgtt ttccttgcac ggataagcgg cgaacagtag caaatgtcaa
     1681 gatgcacgtc cttatattcc tcccgtgcct tttccgccat ttctattccc tcacggcaca
     1741 gcggaatatc cgtcctgccc tgcaattttt tctttttatt ccactcggtt ttaccgtgtc
     1801 gcattatgta tagcattaaa atcctccgta taacgcttta tcagaaaaga tcaagcatac
     1861 tgtcaagata tatttcttct cttactttca gccggtattc cggctttgtc atatagtaca
     1921 gcagaaattc cagcactacc gcactgccaa gccccctgcc ctctattttg aactgattga
     1981 accccatcgg cagataggta ttttcaatat cgtccgttcc gattaacgca gggttttcca
     2041 tagcc
//
LOCUS       DAWAOG010000386         2043 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7161_length_2043_cov_2.193159, whole genome shotgun sequence.
ACCESSION   DAWAOG010000386 DAWAOG010000000
VERSION     DAWAOG010000386.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7161_length_2043_cov_2.193159"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    768..777
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1213..1222
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          624 a          398 c          501 g          500 t
ORIGIN      
        1 gcggcacaca ccgcgcccgc tccgctcccg tacttcatgg cggcgcagag agccagtccc
       61 gacgcaaaga taatgccaaa attgaatatg cctatgctgt agtgggagag aatgcccgac
      121 actatggcac agccgcctat tacacagatg aagctgagag gatcgctcct gacagtcacc
      181 ttgacaagct cgcgctttga cgacagacgt ataagcctta gtgccacgac tgcgccgccg
      241 cccgccatag ccgacaggat aatgcagttc attatatcgc tgacggagga cgaggtgaga
      301 aagttagcgg caaagaccga gcctgccgca gttatagccg ataccgcatc ggaagcggcg
      361 gtttttcggc gggaaacgat aagacgtata gcgactaccg acagcagagc cgaggtgacg
      421 gtcacgccgt ccgaataatt gccgccgaga atatagcctg cgacactgcc tacaaaagcg
      481 cttatgcaat caatgccgct catcgccgcc gcaaatgatg ccgcaaaggg tgacggctta
      541 ccgagcaccg tactcattga aatgactgcc gcgataagca ttaccgaacc gcaccttatg
      601 accgaatgct cacggatatg tacgctgttt tttgttactg cccttgacat tgtaaacttc
      661 ctttcgcaaa tgcagatatt cccgatgaaa aacgaaaaaa gcgggaatat cgatatgtta
      721 ttattttaca attgagaagc ggcgcttttt gtcgcagtaa gtaacggnnn nnnnnnnatg
      781 tcgggcaaag tattatatcg gggcaggcga ggtttttcgc aggaaattga taatatgtga
      841 cggtttttat gtggcggtat caatagcgcg aacgctatag cggcatcaca tttttgcaaa
      901 tacttgacaa ggggtgtttg cggtgatata cttatgatag tatacagtta atactgatga
      961 aatgtaaagg tgagagatat gtgtatgact tcggatacca atttgtgtaa attgggtggg
     1021 ctgagtaaca gcacctctgt aagtaatgca caaaactctt gcgtaaaact caatatggta
     1081 ataaaggcat agtacacgag gggtggactg tgccttattg tattttaaaa ggaaagaggt
     1141 atgtatttat gaaagtgaag aagtggctgc tggggctggt cacctttgcg gcgatggctg
     1201 tgctgtgcgc ggnnnnnnnn nnttccagta tagcgcgctt gatgatggta cggtggagat
     1261 tacagggtat aatggcagtg cggaaaaggt tgatattcct gctgaaattg atgggaagag
     1321 tgtgacaagt ataggcaata gagctttcaa cggttgtaca agccttacaa gcataacgat
     1381 acctaatagt gtgacagaga taggaagcgg cgctttctcc agttgcacga gccttacaag
     1441 tataaaaata cctgacagcg taatgcaaat aggtgactat gtttttgtgg ggtgtacaaa
     1501 tcttattgaa atacaagtag aaacagataa taaattctat tcttctgata aaggtgttct
     1561 ttttaataaa aacaaaactg aaataatttg ttatcccgca ggaataaaag atactatata
     1621 tctcatacca agtagcgtga cgagcattgg caaaagagct ttccaaaact gctcaaacct
     1681 aataaatatt aaaataccag atagggtgtc atatataggt tcaattgctt ttgccgattg
     1741 cacaagccta acaagtataa cgataccgaa cagtgtaaca agtttaggca atagtgcttt
     1801 cagaggttgt gcaagtctta caagtataac gataccggac agcgtgacga gcataagtgg
     1861 tggtgctttc ggtaattgca caagtcttac aagcataacg ataccggaca gtgtaacgag
     1921 tataggtgga aatgcattta gtaataccgc attactaaaa aatcaaacaa catccgaaaa
     1981 atacgtaggc aaatgggtta tagattgcga tgatgatgca aaaagtgtca caataaaaaa
     2041 tgg
//
LOCUS       DAWAOG010000387         2041 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7168_length_2041_cov_2.233132, whole genome shotgun sequence.
ACCESSION   DAWAOG010000387 DAWAOG010000000
VERSION     DAWAOG010000387.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7168_length_2041_cov_2.233132"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          481 a          465 c          438 g          657 t
ORIGIN      
        1 tttgcagtga ctgcgattat cggcacgttt tttgcataat ccgtattcag gcggcgtata
       61 gctttagcgg cggcagatcc gtccattaca ggcatctgca tatccataag aacggcatcg
      121 tagcaaaaat tttcggcact cgtaaacata tcaacgcata ttttgccgtt ctctgcatgg
      181 tcgcaggtaa tgccgtgcat ttcaagctgt tcggaaagta tttcgtaatt aagctcgttg
      241 tcctctgcca caagaagatg catacctttt cctaagcagc tgtcttcttt ttcattttca
      301 tctgaaatgg cggtttcgtc aaggtacggt atgtcgatcg tgaggcggaa ggaagtacct
      361 tcctgcaact tgcttgtaac ttcgatcgtg ccgcccataa ggtcggagag tttttttgct
      421 atcgcaagac ccagaccgct tccgcgtact ttgtttactc gggtatcaac ggcacgggta
      481 aactcatcgt acattttgct cataaactct tcactcatgc ctatgccggt gtcgctgacg
      541 attacaacaa gacgtgtgtt ttcaggctca tctgagcttt cttcatacat ttcaagactt
      601 accgttccgc cgtccggtgt gtatttgacg gcgttagaca aaaggttgat tactatctgg
      661 cccaatctta tgcggtcaac gctgacaata ttatgttcga tatcgtgcag gtcacagttg
      721 taatacagct ttttgcctat tcccgcgccc agcatcgact gcttgaagaa atcaaatatc
      781 tcggttaaac aggacggctc ggcgttaagc tcaaagtgac cgctttctat ctttgacagg
      841 tcaagagtgt catttatcag ctcctgcaga ttattgccgc tgtattttat cttttcaagg
      901 cagtcggcga gcttttcacg gttgtcgagg ttttgtatgg ctatattgac aaagcccata
      961 attacgttca taggagtcct tatgtcatgt gacatccttg acagaaagtc ggactttgct
     1021 tcatttgcgc ggttagcttc ttctgcggct attatccagc tcttttcacg ctgcttttct
     1081 tcggttatga ttcttacaac aaacagtaac tttgccggat cgccgttttt gtcacgttcc
     1141 tggacaataa acctagcaag atgccagttg ccgtcacagg taagatattc cgtgcccgtg
     1201 gattcgtcgt tcttaaggcg ctctttcagt gtagcgatat caaagaaagc atttgctttt
     1261 tcccgatatt taggagccac aaagctgctg cacatcgatc tgagctcaaa tgacgctttg
     1321 ccttttttgc ctgtcagacg gtgttctgcg cttccgctgg atatttcctc aaagcagtct
     1381 gcaatgatat ctatacgata gataaggaag tagatcttac ttatcgcaaa gatgatatcg
     1441 ttgttaaact tggcaagttt cagtgcggat tccagctttt cgcggcgttc gcattcttct
     1501 tttactattt catctatatg gcggaagtca agaagtatca gaaatttatc gctgtccgat
     1561 tttatctttg atgcccttgc ttcaaaaaac acctgaccgc gtttattggg gacgctcata
     1621 aagcgtatgc ttatcctgtc tttatcgctg agttctttca taaggttttc acggctcagc
     1681 tttttgagaa agttcggcga agagctgttt acaacataat tgtcataata ggacttgatc
     1741 gtttttgaat aacagatcgt tatcctttct tccgacctga taaaattgaa aacgttagtg
     1801 tagctctcta ccttgacgac ctgtgcagta tcgttgataa gatcgacacg gtataccgac
     1861 gcattatcgg cacacagcac cataagtata tcgttttcgt tttgtatatg agcaatattg
     1921 ttttcaagct ctttctgctt tttccgaagc aaagtgagca ttttcatatt ggctctggag
     1981 atgccgatat gagtacctac gagcgacagc agctttataa gcagctccgg ctgtccgcag
     2041 g
//
LOCUS       DAWAOG010000388         2037 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7190_length_2037_cov_2.287084, whole genome shotgun sequence.
ACCESSION   DAWAOG010000388 DAWAOG010000000
VERSION     DAWAOG010000388.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7190_length_2037_cov_2.287084"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          460 a          540 c          410 g          627 t
ORIGIN      
        1 gggaacatag tcgcttgccg cacatacaac ggcaataaac ggcttatgca tctcactgtc
       61 tgttacaccc agtgccttca gtaaggcttt gtgcggtgtt ctgtctacgc cgctcttgat
      121 aagatcgctt ctcattttta ctgtcctttc cttttgtaaa aaagtgtttt tattttcggc
      181 cgagcatggc actgccgatc atacctgctt catttgcaag cgtgcagatt actatctcgg
      241 tatttttagc attgtttttt gagtagatct gctttgccac ctgtgctttc agcggtataa
      301 gcagattatc accctcgtta catactcctc cgccgataca aagaatatca ggctggaata
      361 cgtttatgac atttgtgata ccgcaagcta agtactttat atatttttcg actacttctc
      421 ttccctcggg atcgccgagc tttgcggcat tgaatgctgt tcttgcggat atcttaccgt
      481 catggtcaac catttcgtga agccgtccgc tgggatattt ctcaatcatt tccctcgtca
      541 tacgcacaag tccggtcgcc gaagaataag cctcaaaaca gccgtttctt ccgcaggtac
      601 acggcgcacc gtcaaccttg ataactgtat gacctatctc gccgcccgcg ttgtttgagc
      661 cgctgtatat ctcgccgttt ataattatac ctgctcccac acccgttccg agcgtcagca
      721 caacggcatt ctttgcgccc tttgccgcac ctgcaaggaa ctcgccgtat gccgctacat
      781 tagcgtcatt ttcaagtatt atcttcttag cgaaatgcgc ttcaaggtct ttagcaagcg
      841 gaacgttctc ccagtgcaga ttatttgaat acagcaccgt gccgttttcg ttatccatag
      901 ttcccggaca gccgacaccg atagacttta tcttgtcgat atcctctccc gaaagctgta
      961 cagcctgttc aaccgcattg ataattaccg caagaacgct tttatagccg tctgccgcat
     1021 ttgtcttgag tgatattttt gaaacgagct gtgcgttctc atcgaccact cccgccttga
     1081 tatttgtacc gcctatgtca acgccgatat aatatttcat atttttccct ttgacctttc
     1141 acaacgcaag tcttaaacta tatcagaaag aattacctca aggttgcctg taagctcgta
     1201 tttaccgtag cctgccggga taaagaagct ttcgcccttc tttacatcaa gctcgccgac
     1261 cttgccgtta ccgtcaagaa cgagtatcga atggaacgac ttttcatcag cgtcaagagt
     1321 taccgtgccg tcaaccttta tcttatgaac atcgaaatat tcgcaggtgc ggagcagtgt
     1381 ttcctcgccg ccgtctatct tgacaggctt accctgcggc tttgtatcgt atttcggagg
     1441 gcaaagctct gtaacatcga tagccttttc aacgtggagt tctctaggct tgccgtcctt
     1501 gcctactctg ccgtagtcat atactctgta tgtcgtattg ctgttctgct gaatttcggc
     1561 gataagtata cccttgccga tagcgtggag tgtgcctgat tcgatgaaga acacatcgcc
     1621 cttatgaacg ggaacgttgt ttgtgacctc aagtaaggta ttgttcttaa ttctgtcggc
     1681 aaattcttct cttgaaattt ctttatcaaa accgtagatc agctgtgagc cttcctcaca
     1741 gtcaacgata taccacattt ccgtcttgcc gtactcaccc tctacacgca tagcgtaatc
     1801 attgtcgggg tgcacctgaa cagacaggtt atccttagca tcaataagct ttataagtat
     1861 ggggaagtac tcaaatctgt cgcaggcagt accgagcgat ttcttattct cggcaacgta
     1921 ttcagaaagc gtcttaccct tatccttgcc gtttgcgata acgctctgac cgtccttatg
     1981 gcaggacagc tcccagcttt cagcaatctt atcagcgtct gataccttgc cgtattc
//
LOCUS       DAWAOG010000389         2035 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7201_length_2035_cov_2.078283, whole genome shotgun sequence.
ACCESSION   DAWAOG010000389 DAWAOG010000000
VERSION     DAWAOG010000389.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7201_length_2035_cov_2.078283"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1024..1033
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          562 a          432 c          420 g          611 t
ORIGIN      
        1 cagtgatggt cggtacggta gtatatatat tcgtttttag ccgcaagcag tcccgagtac
       61 acgtctatac ctgttatatt gggcaggctt tcatagcagc ttttgatgaa cagcttctga
      121 tcggcagccc cgcaattcgc aggaagtgtg tcactgtata tttcctgtgc gttaggagca
      181 agcataaagt acatttttat cttagggtac ttctcggcga atttctccat tgccgaaaga
      241 tttttgttta cgctgtcgac actgtagtcg ccggttctcc atgactgcat cattctgccg
      301 tcctcggtaa atattccctt tatctctttt ttaccgagta tgcggtcaaa gtcattcata
      361 agccctatcc attgctcacg catgactatt ctgtctgcaa aaaagttttc gaattttgtc
      421 ataaatgact tgtcggttac tgtatcaaag ttgaactccg gcattttttc aagatatctg
      481 ttttcgtttt cgctgaatgc attttcaggt aactgtgcgc ttgaaatgat tgttgcaaca
      541 ggtactgtga tcgagataag agcaaacagt ccgatgagca ggatcttata gatattctct
      601 tttttcattt tgtctgttcc tttctcagaa attaaaatac aggaacgggt tatatgtctg
      661 gtcgacaaga tatgccgcgc acataacgaa cattcccatg atggcaacaa ttccgccgta
      721 gtttatgatc ggtgggattt tcttttttat aaattccacc ccaagtttag gcaggtcggt
      781 acatcctatt atgcagatta tgaatgtaat tccgtatgtt gctatctggt acattgcggt
      841 gctgtctata aacggtgtgc cgccagcgcc gaacatattg gctatataag cggcggctgt
      901 gggtaagtca gccgtatcga acagtaccca gccgagtatt acaagcagca ttgtataaat
      961 gtggcagaat accgccggaa ttttctgcaa ccactttccg agaaaaagtt tctcaaggat
     1021 gatnnnnnnn nnngatgata acaataccat aaagggaacc ccaaagtatg aagttccagc
     1081 ttgcgccgtg ccaaacacct gtcagaaacc atgttattgc aagattcata attgttctgg
     1141 gcataccttt acggtttcct ccgagaggaa aatagacata cgatttgaac caagaaccaa
     1201 gtgtgatgtg ccatcttcgc cagaattcag aaatactttt agaaagatag ggataattga
     1261 aattttcggg aaagtggaag cccatcattt taccaagacc tattgccata tccgagtatc
     1321 ctgagaagtc aaagtaaatc tggaatgtga atgcaagtat tccgagccac gagcttacta
     1381 ccgatacctg agagatatcc gtgcccttga ccgccgtcca taatgcacct atactgtttg
     1441 caatcagtac tttcttagaa agtccgatga taaaacgtac gatgccgtca tatatgaggt
     1501 caatagtaat gcttctgtcg tcaagttctt ttgccacatc gtcatatctt acgataggac
     1561 ctgcgactat ctgcgggaac agcgtaacga atgccgcaaa gcttatcgga tttttctgca
     1621 ccttaacatt ccccatataa aggtctatcg tatacgacat agactggaat gtgaagaaac
     1681 ttatacctat aggtagcata ttcattggta ggaagtctat accaaagaag ttcatattgt
     1741 ttatatcgat tatctgactt ccggctattg cgttgatgtt ttccgcgata aatccgctgt
     1801 acttaaacac cccgagaagt ccgaggttca ttacgaccga cactatgagg catatcctgc
     1861 gagggagttt tttcccctca aagtgattca tcacaagtcc ggcaaaatag tctatcatcg
     1921 ttgaaataag cattacaatg acatatatcg gctcacccca tgaatagaag agaagtccgc
     1981 tgagcaggat tatcaggttc ttgccttttt tgggcgtaat ataatatatc aatag
//
LOCUS       DAWAOG010000390         2022 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7267_length_2022_cov_1.714286, whole genome shotgun sequence.
ACCESSION   DAWAOG010000390 DAWAOG010000000
VERSION     DAWAOG010000390.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7267_length_2022_cov_1.714286"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          698 a          427 c          446 g          451 t
ORIGIN      
        1 gcgtcaagat tgttctcgat caccgaattt aatttgtgaa attccttgtg tgaatcggat
       61 actacaacaa ttttcataat ttctgtcctt tcagtcgggc aagccgcata tggctgaata
      121 taaacccata aagtaattat atcacatatc aggaaaaaag aaaagataac tatccgtatt
      181 tttagaaatt atacacaaaa cgcattacgc cttttgtata attttacaaa aaattttaaa
      241 ctgaatataa cgggaatatt cccatcccgt ttaaaatata atttcaggag gctgttatga
      301 ataataatat tgattctttg cttgatactg tcagcaaaaa acttggtata accccgcaga
      361 aactaagaga agcgatgcaa aagggcgacc tttcgatagc actgcaaaat atgcctgaaa
      421 aggacgcaaa aaagcttaaa agcattctcg gcaacccgga gcttatcaaa aaaatgatga
      481 ataccgcaca agcgcaggaa ctgaaaagat cgatgaaaaa gcagtaatga acggaggaca
      541 gctatgagtg agatagatga aattcttaaa gccgtgctgt ccgataacaa aaccgacaaa
      601 tcaacctcag acgaagcagg tcagacggat aacgaagaaa aattcacgac cgacaccgaa
      661 agcgatgata ttttcggcgg aataaacata gatatgttta tgaaaatagg cgagataatg
      721 tcaaagatga gcgagcagga caagaacacc gagctactgt tggcgttaaa gccgcacctg
      781 cgtgatgaaa atcaggcgaa gatcgacaca gcggtaaagc tgttcagact tatctctctg
      841 ttgccatatc tcaaagacag cggtcttttt gaaaatctgt tatgaaaagg agttacatta
      901 tggaatggaa cagtataaaa atgtcaaatg ctgagtttga acgcttgcag aaagaataca
      961 cagaagccgc tatgaaagcc gccgaaaaag cgaagcattc gcaaccgacc gaggaggtat
     1021 cgattgaagc tccgcggtct gtaccaccgg ttcataccat cacagaagcg gtactcgccc
     1081 cttcaaatga aaacgcagac gtgcccgaac ctgccgaaaa cgagataacc gaaaccacgg
     1141 atctatacga aaacgatatt gatatttagg cagaagccgc agaatacagc gcagaacaaa
     1201 attcagccag aactgagccg gaaacgcctg ataatgacga aacagcggac tgcgatgaag
     1261 atgccgaaac accgcaggat acatcggatt ttcagcagga ggaagaaatt tgcaatagcg
     1321 aaagcttgaa aaccgatgat cccgatgact gcggcacaga ggaggagcat accgatgagg
     1381 agatacttga cagctttgca agcgttttta tgagtgaaga tgaagccgac aaaaaagtcg
     1441 aaaagctctc cgccgacaga cagaagagct accctccccc gcgcttcaac accgccatac
     1501 ataaccacaa taagagtatg agcaataaca aaaagccctg cggatgtgag cactgccgct
     1561 gtcaaagcgg cagtcatatg caggagaaag gcgggcataa acctgactga aattccgccc
     1621 gcagtaaccg aatggacata atcggaagaa ttatgaatga cgatgacctg cttaaggtcg
     1681 ccgctgtagc atttatcctt tggcgcgata aagccgatta caagctgctg cttgtactgg
     1741 catacgtttt tctttttaaa taaatcttgc attttcccgc ccgtcagtat ataatatcct
     1801 tataaaacgg ctttgacgca atggaggtaa attatggcta aggggctgga aaatgcaatt
     1861 ttttatgaga tatacccgca gtccttcaac gacacaaacg gtgacggtat cggcgatttt
     1921 cagggtatta tcgaaaagct tgactacatc aaggaaacag gcttcaatgc aatatggatg
     1981 aacccctgct tcgactcacc tttcggtgac gcgggctatg ac
//
LOCUS       DAWAOG010000391         2021 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7269_length_2021_cov_2.442014, whole genome shotgun sequence.
ACCESSION   DAWAOG010000391 DAWAOG010000000
VERSION     DAWAOG010000391.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7269_length_2021_cov_2.442014"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          620 a          449 c          452 g          500 t
ORIGIN      
        1 tcgccgtact tcccgtagag tgtacggctt atgcccatcg tcaccgcaaa catacacggt
       61 cctgcaatat ctccgactga tttagtcagt ccgagagcgg attctgcaaa tgccgacgcc
      121 cactgcgcca ttgaaagctc cgaggctccc gcacaaacca taagcagtat cgcaagccag
      181 aacagcggta ctctgagcag gtcacggata cgcattccct tgccgttctc gacaggatgt
      241 tcaatgggac atactgaaaa attgtatatg ttcacaagtg gaattatcgc ccagatacaa
      301 gagagccact tccagttttc tatgccgaaa acagtaaaaa acagtgttga aatgagtatg
      361 actccgaccg aaccccagca gtagaatgaa tgcagcaggc tcattgcagc ttctttgtgc
      421 tcaaacgggc aggcttcaac tatcggactt acaagtacct cggtaagacc gcttcccatt
      481 gcatatatga ttgtgcttat gattattcct gtgaacgggt ctggaagcag ttcaggcaaa
      541 aaggcaagtc cgataagtcc cagtgccgaa aaagcctgcg aaccgataac gcatttccga
      601 tagccgatct tgtcggcgaa acgtgcgcac agcacatcta tgataagctg agtaacaaaa
      661 aataccgacg agataagggc aatttttcca agcgggatac catagccatt gtgaaatgtg
      721 agaaatagaa gcggggcgaa attagccgct attgcctgcg tgatgaatcc aagatagcag
      781 gatataagtg tttttctgta attcacagcc atatagacaa actccttgac gattgatata
      841 atcagattat atagcaaaca tcacaacatt tcaatgcacg aaatcacatt tttattacac
      901 aatatcgtaa atcaggtgag aatatgagtg atgaaatcaa aaaggacagg atcataacgg
      961 atgaaacctt acgtgaaact gcaagtcacg gcagcgaaga atatccgttc tgctactatt
     1021 atgaggacat ctgggatttc gactttcact gtatcgactg gcactggcac ccggaggtcg
     1081 aatttgttta tgtacagaat ggcaaggctg attttctggt gggtggaaac aggtatgttc
     1141 tcagctccgg cgatgggatc tttataaatt ctcaggtgat acacaggttt gaagcccagg
     1201 acagcgccat cattccgaac attgtttttt cacctgttct gcttgcgccg cagggcagtc
     1261 tgatatacag cagatatatc agaccgataa ttgaatcaac taccgaatgt gtgatttttt
     1321 ccgccgaaga aacagcgcac cacagcatta tcgaaacaat gaggtcggta tttgccgtgc
     1381 agggttcagg cacagcgtct gaaatggaaa cagcagaact gctgctgaaa ctatggcgcc
     1441 tgttatatga aagcatacag ataacagatt gcggttctat gtccgtgcat tccgcacaga
     1501 cgcaggctaa gcttcagata atgatgcagt atattcatga taattacagc ggtcagataa
     1561 cactggacga tattgcccga acggttctta taagcaaaag cagtgttctc aatattttca
     1621 ggacatacct gcatacatcg cctataaatt acgttgtaga atacagactg aaacgtgcgt
     1681 caaagctgct tgttgatacc gaaaacagcg tttgcaccat agcgcaggaa acgggatttg
     1741 agaacatcgg ctatttctgc cgcaagttca aaaagctgta cggcgttacg ccgataaatt
     1801 acagaaaaca ctgtcgcagt gcggagcgta attcaatgag aaatatacat tctgacacaa
     1861 atatcctgtc gggctgataa tacattgcca cataaatcaa aaaaattcac aggcgacata
     1921 ataaaaaata gtatacctac tgtttattag gtgacaatta tgccaaagag cgtcgggcta
     1981 tctgtacagc tttttcattg aattgagccg ccttgcggcg a
//
LOCUS       DAWAOG010000392         2021 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7270_length_2021_cov_2.372838, whole genome shotgun sequence.
ACCESSION   DAWAOG010000392 DAWAOG010000000
VERSION     DAWAOG010000392.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7270_length_2021_cov_2.372838"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1542..1551
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          746 a          331 c          435 g          499 t
ORIGIN      
        1 acacaggaga tattgtatat gtaatggaag actatatcct tactgaatct tacaacaata
       61 ataaacctct cgaatggctg gatgaaagca taaaagatat agaaaaagtc attgaaaata
      121 attccgataa ctatttggcg gaacaatttg gtgaggcact tgaatctgat aaaaaatatc
      181 gtgactatct gacgtcacat cagactgtaa tatctaccag aacggtttta ttagaaaaag
      241 ggcagtcata tttaatgatt tttgatgaaa aagcaagggg ttttgtattt gacaataatc
      301 aatatgccgc aaattacaca gtgacttttg accttgaaaa cgatgcgccg aaagtatata
      361 tgagcgagga agaagatgcg gactatttca atggctatta ccgttacaga gatcagtgga
      421 aatatcttaa agaaaaatac ggggatcatt tcaagaaata acgcgggact acaaaattga
      481 ggtaaaacgc tatgaaaaaa ctaaccaaaa caacagcact catcttatcg gcacttatgt
      541 tattcggctg tacaagtaac tctgcacagg tcggtgtagg tgatactaca ccgccgagcg
      601 agagctcagt cacggacagc aaaaacgaaa gtaaggttga aagcccggtt actgaaaaca
      661 gctctgtaac tgataataca agcaaggaaa gcgaagaaaa ccttgtgaag aatatcgagg
      721 gatatcagac gttttcgtat gatgagaata aatacgaaaa tttcaccgaa atagaatata
      781 cccaacatta ttacggcgtg tatgaccgcg ccggttcatc cgatcctacg aaaaaagaaa
      841 atatgaaagc aaacaatcga agctctatag gaataaaatg ccgggttgta ggtgacagct
      901 attatgtaca cggaggtgta tgcggtaatg tcaaaattac cagagaagag gacgactaca
      961 tatttacacc tgttataatt gaagaaattg tcgaggactt cggaaatgaa agtgtatttt
     1021 ctgttggcga tataatttat gtgcgggaat ggtatcgacc ggttgaagaa tacaatacaa
     1081 aaacgcctaa atgggtggaa gagcaaattg aatatataaa acagcaatat gaagatgaaa
     1141 acggcaattt caacagcagt tattttagtg aagaaaattt tgagtattat aaaaaacagc
     1201 ttgaatacta tgatattatc ggtaaaaatc ccgaaaaata tgttgtcaat ctatgtgcac
     1261 ctgtaatgat gcaacgaggt cagtcgtatc ttgtgtttgc agaaaacgtt aaaacgccga
     1321 atgaaatcga cgggaatatc tattgttata catataacat aatatttgac cttgaaaacg
     1381 acgctcccta tgtagatcag tcggaagaga tggttaaaaa tctgtaccga tgcggagctt
     1441 gctatcagaa tcagtggaag atacttaaag aaaaatacgg ggagcatttc aaaaaataac
     1501 aagggactac aaaattgagg taacaaatta tgaaaaatct annnnnnnnn nactaaccaa
     1561 aacaacagca ctcattttat cggcacttat gctcttcggc tgtactgata attctacgca
     1621 gagcggtgta ggcgatgcca cgccgccgag cgagagctcg gtcacggaca gtaaaaatga
     1681 aagcccggct actgaaaaca gctccgaaac agataataca agcaaggaaa gtgaagaaaa
     1741 ccttgtgaag aatatcgagg gatatcagac gttttcgtat gataaaaaca agtatgtaaa
     1801 aactactttt cgcagaatat ataaccttaa aagagatgga ctgggagcgt ttaccggtga
     1861 aacagtaaca cttcctcttg cacgatatgc agtacaagat gaggtgttgg taataaaatg
     1921 caaggttgcg ggcgatagct atgtatttca gaatggaagg agcggtatca aaaccgtgca
     1981 ggattcttct gatgataacg ttctgaacgg agtatatact c
//
LOCUS       DAWAOG010000393         2021 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7272_length_2021_cov_2.080875, whole genome shotgun sequence.
ACCESSION   DAWAOG010000393 DAWAOG010000000
VERSION     DAWAOG010000393.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7272_length_2021_cov_2.080875"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    278..287
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          660 a          380 c          458 g          513 t
ORIGIN      
        1 aatataacga taccggatag tgtagagtcc ataggctatg gagctttcgg gtcttgtaac
       61 agcttgacaa gtataacaat accagacggt gtcgaaatta tagacgaaat tactttctat
      121 aattgtttta acttaacaag catatcgata ccagatagtg tgacttatat tggcaaaagc
      181 gccttttata attgccaaag cctaacaaaa attaccctac cggatggtat aacgaatatt
      241 gaaaatcaag ctttcaaagg ctgttcaaac cttatagnnn nnnnnnncct tatagatata
      301 actatcccaa gtagtattga gaacatagat gactgggttt tctacggttg ctatagtctt
      361 acaagtataa cgataccgga caatgtgacg agtataggcg aaagcgcttt ctactgttgc
      421 aagagtctta caagtataac aataccgaat agcgtgacca gtatagatga tgaggctttt
      481 gaatattgcg ataacttaaa acacatacat attccttacg gcactaatta ttccgaatat
      541 tcaggaaaag gcagtttgcc gtcagacccg gaatattact ttgtgcttac ctcaagcggt
      601 cattgtcctg atgaaagttg tcctgacggt ttgtatcaac ctgacataga ggacgaccgc
      661 gacaacccca acggtgtaat aatcaatgac ggctcatatt ctctgagtga ctccaatgcg
      721 acaagcaacg gtgttatgag cagcatttca agcggtgaga aaaaggatat gacggtcgac
      781 ggcggttctc ttgtgattga cggcgggctg ggcaagacat ataatatagg tacgcttact
      841 ataaaaagag gaaaactgac gatccgtaac cctgttgtaa acattgataa tcttatcata
      901 tacggcgaca gtgagtacga cggtctgtgg ataaattcaa gtgcaaaagt aaacgttaat
      961 atgtatgccg atgtaaccgg cggttacaac aggctgttca cgctatggaa gaagcagggc
     1021 ggaacgttga gcgtcagcgg cactcttaac gtaaaccgtg accttacaat agaaaagggc
     1081 ggtaatttca cacagaatgc aggcagtatt ataaaggtca acggtaatgt tgagttcacc
     1141 tcatgctatg cacctgcact gaaaggcggt acgctctggg taaagggtaa cttcacccat
     1201 aaatcggcga agactaccgc ggacagcaat cataagacca tattctttgg aaataccgat
     1261 aagaagctgt ctgttggcag ttttaaattc ggaaagcttt atcttacaaa tgaagccgat
     1321 aacggtaaca atctgaaaaa cgtactgcgt acagagaacg caccgtttgg aataatgact
     1381 tgtgacgcaa acggaactcc tgtcgcttca agaggaaaat atctgaagga ttatgacggc
     1441 tcaggtgaat ggtacaacgg ctggataggt tctgtgtcaa ggcagtttga cagttacggc
     1501 tcggtcagca ataaaataac aggtattgag atagtcgaca agagcgttga agaatcgata
     1561 tacgcaaagg tagaaacata tgctatgctt atggctaccg ggctgcctga tgatatgaca
     1621 ggcgacagca gaaatacctg cattacattt aaaaatatca aggtcaacta taagggtaaa
     1681 gaaaagaagg ctacaatttc gtttgtgtat aaggacgcaa tagcgaagct taaagaatat
     1741 gccgcattgc tcgacacttc gttcccatca gacggtatgc atctgtcaag cataagctac
     1801 tttgtcaata taggcaatga gtcatttacc ggaacatata aaatgacggc ggctactaat
     1861 cagaagctgt ttgcacagga gtgccttgat attatagaca agggctggaa agatgatgtc
     1921 aagagctatg taaagactta ttccaataag ctttgcggcg tggcatacag taagattaag
     1981 aaacccgaat ttgtaaatac gcttatgaac gcgtataaga c
//
LOCUS       DAWAOG010000394         2014 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7307_length_2014_cov_2.240429, whole genome shotgun sequence.
ACCESSION   DAWAOG010000394 DAWAOG010000000
VERSION     DAWAOG010000394.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7307_length_2014_cov_2.240429"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    694..703
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          624 a          422 c          452 g          506 t
ORIGIN      
        1 gacctgcatc gattatgaca agatcccagg taagacccgc atctgcaaac ctctgcataa
       61 gagaattatc ggatgcaagc gcttcttctg cgattatata tgtattcggc atattctcgt
      121 tgaagtaggt gatagctttt gatgaaccgg atatcatctt gaaatcaacg ccgtttgatg
      181 ttactaccga gcgataccag ctatgcataa gatgcctggg gcatatcagc aggatattgg
      241 gagtctgctt ttcaagcacg gtatcgagta tgcttatctg agcctttgtc gatttgtctg
      301 cgccgtattc gtcgagaaca aggcaaaagc cgaatttatt taacttttcg tgaaaagaac
      361 agctgatcgc acttatatct ttgccatagt acgatatcca ttttatctct tcggtattca
      421 ctggatcacc ctccgctctg cccgcataca caaaccgggc tgaataatat aactattata
      481 atacatttga gcatttttta caaccgatat ttcgcatttt atagaatttc aataaaaatt
      541 tgcacacgaa tttagcaaaa agctacaaat gcctgatcgg tttactctat atgattataa
      601 gaaaaaattt acggaaatgt cttcactttg ttcgacttta cttgacaaaa cacgataaat
      661 atagtatgat actggtatat gtcaaaaggg agtnnnnnnn nnnatactga ctgttacggc
      721 tttgctcata attgtgccta cggtgctttg tataattttt gctgtagaca atgccaaaaa
      781 gtcggaagag atagacaggc tcaattcacg tctgtcggca cagtcggaag atatatcggc
      841 ttcggcgaca gaaagttccg atgactctaa aggcaatagc actgcggaga acacaaataa
      901 agcggaacct tctgtgaccg aaagcaagcc cgctgaaacg actacagcgc agacgacacc
      961 cgttgcttca tcacagacag aaacaacacc ggacacaaca aaagagccgg caagtacacc
     1021 tgagagcagt gatgaaacat caaacagcag tgccgatgat aagttgtata ctgaacttta
     1081 cccggagctt tatgcggata aatatgacgg tgaatatgaa gacagcaaaa acaccgtata
     1141 tctcacattt gatgacggtc cttcggtgct gacggaaaat ctgctgtatt atttacggca
     1201 ggaaaacgta aaggctacat tctttgttgt tcccgagcgc acggagtatt gttattcgtt
     1261 actgaaagaa ataagtgacg cgggacatac gataggtatc cattccgcct cccatgatta
     1321 cgaaaagata tatgcttcgg tagacgcgtt tcttgacgat ttcaaggaag cgtatgatat
     1381 tgttcttgaa gcgacgggaa aagcacctga tatatacaga tttcccggtg gcagtgtaaa
     1441 tgactataat gcggctacgc gcgacgatat aattgcggaa atggaccgca gaggatttac
     1501 atatttcgat tggaacgtgg acagcaacga ctggcaaggc tatggctgga caacgcttta
     1561 cacaaatgta cttgcagacg cacaggagtt ttcgacaccc gttatactgt ttcacaacac
     1621 aggggatcga gataataccg tacttgtgat agaggatatc atcaaggcgc tgaaagataa
     1681 aggatataaa ttcggcagtc tttcgcagaa gaccgaaccg atccaatttc aagaaaggga
     1741 taacaatggg aatcaaggat aatttttcac aggctatgaa ggagcttaca aatcccagat
     1801 cgtccgataa ccgcaaagag gtcaaggatg ttgcggcata tatgaatgat actgccgaca
     1861 cacagccggg tgaacctgca cagaatgcgc agcctgctca ggataacgta cagccctcac
     1921 aggcgttcgg atcaagcaca caagagcaga acgttcagaa cacacagcag ggaacagcag
     1981 caggcggatt tgtgggacag gatttttcgc agcg
//
LOCUS       DAWAOG010000395         2010 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7328_length_2010_cov_1.937596, whole genome shotgun sequence.
ACCESSION   DAWAOG010000395 DAWAOG010000000
VERSION     DAWAOG010000395.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7328_length_2010_cov_1.937596"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1050..1059
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          561 a          479 c          463 g          497 t
ORIGIN      
        1 gaaaggcaag aagcacataa tcacttctgt atttgagcat cacgcagttc tgcacacact
       61 ggatgcgctc aagaaagagg gctttgaggt aacatacctc cctgtttact caaacggtat
      121 agttaaagtc gaggacgtag caaatgctat ccgccccgat acagcccttg ttacgataat
      181 gtacgccaat aacgagatcg gtacgatcca gcctattccc gagatcggtg cactgtgcaa
      241 ggagaaaggc gtgatcttcc atacagacgc agttcaggct atcggtaacg tccacatcaa
      301 cgtaaaagag cagaacatag atatgctttc cctcagcggt cataaaatac acgcccagaa
      361 aggctgcggc gttctttatg taaacaagaa ataccgcctt cccccgttca ttgacggcgg
      421 cgctcaggaa agaggcagac gtgcaggtac agaaaacgtt cccgcgatag taggtcttgg
      481 tgttgctata acagacgctg taaacggcgt tgacgcaaag aatgagaagc tcaagaagtt
      541 tagcgacaag atctacagcg agataatgaa gattgaccgt gttcacctga acggcgacag
      601 agaaaagcgt atgccgagta acctcaactt ctcattcgag ggcgttgagg gcgaaagctt
      661 actgctccag ctcgatcttc agggtattgc cgcttcaagc ggttcagcct gcacatcggg
      721 ttcactcgac ccctcacacg ttctgctgag cataggactt ccacacgagg tggctcacgg
      781 ctcactgcgt atctcaatgt cagagtacac gactgaggaa gagatagaca agctgattga
      841 ggttctgcct gttatagtaa acaagctcag aagtatgtca cccctgtggg atcacataat
      901 caagggtgaa acattcaaga taaagtaagt atacactata tttgaaagga agtattactg
      961 atgatttatt cagaaaaggt aatggatcac tacgctaacc ccagaaacgt cggcgagata
     1021 gaggacgcaa acggcatagg cgaagtaggn nnnnnnnnnt aggtaacgct aagtgcggcg
     1081 atataatgaa gatgtacctc aagatcgagg acaacacaat aaaagatgta aagttcaaga
     1141 cgttcggttg cggtgcggct attgcaacaa gctcgatagc tacggaaatg ataaagggca
     1201 agcccttatc ggaagcgctt aagcttacaa acaaggcggt tgtcgaggca ctcgacggtc
     1261 tgccgccggc aaagctccac tgctctgttc ttgcagaaca ggcgatcaag gccgctattg
     1321 cggactacta cacaagacag ggtatcgatc ccacaccttt tgtaggtgag ataaaggatg
     1381 accactgcga cagctgctgc ggtatataac cttataagta aagagccttg cggcatttgt
     1441 gccgcaaggc tctttcaata ttccctccgt aaaactctgt gatgaattta cagtcagtaa
     1501 tctcaatcgt gcttgcctat catcttttcc atccacacca tatcataata ccgtccgaat
     1561 ttctttgcgc atttgcggaa tgttcccaca agttcatatc cgagcttttc gtggaacatt
     1621 atgctgtcgc ggtttagata ttcatcatca ttttcgggac acgctataca agcatatgca
     1681 ttcttgatac cgatagccgc aagccttttt tcaagctcat tataaaggag cgtacctgtg
     1741 ccgcggcgct tttcgttcaa atcaacatat atcgatgttt cagccgagtg gtcgtaagcc
     1801 gccctatcct taaagacccc cgcgtaagcg taacctatta ttttgccgtc acgttctgcc
     1861 gctatatatg gatatttctt cagcgttttt tctatcctgc ccctgaaatc atcaacagtc
     1921 ggcacttcag ctcataggtt atcgctgtgt tttcaatata atatttgtat atgccgagca
     1981 gcattccggc atcatcgggc gttgctgtcc
//
LOCUS       DAWAOG010000396         2010 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7326_length_2010_cov_2.502302, whole genome shotgun sequence.
ACCESSION   DAWAOG010000396 DAWAOG010000000
VERSION     DAWAOG010000396.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7326_length_2010_cov_2.502302"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          609 a          406 c          472 g          523 t
ORIGIN      
        1 ttctccacat accaaaaaat gagccgcgcg cactatcctt tgcgcttcga ctcacttttc
       61 cttttttagg ttgtgtcaca ctccccgctt ggcgcggaat agtgcaacga agaaaatagt
      121 acagtacgtt aaatctttgg ctaaagtcaa tgcccggggt ttgcgtatag gtgaatgaaa
      181 catctgtagt gcgaatatga aatttatatg gcggagaatg agggtcggcg cgcttgtttt
      241 tgatttatgc tattgatttt tgcgttctgc tgtggtaaaa tattatagaa tatagtcccg
      301 tattataaaa tcggaaaaaa gggacacaaa cggtatacaa gaaaggaaac aaaaaatgag
      361 taaggtagtc aagttcggcg gcagctcgct cgccgatgca aatcagttcc gcaaggtagc
      421 tgatataata agaagcgaca aggaacgcag atatgtagtt ccgagtgcgc ccggcaagcg
      481 tttcaaggat gatatcaagg ttacggatat gctctatgcc tgctatgacg cagtaacgaa
      541 cgaaggtaag aatgcataca atcagtttgc acctattgca gagcgtttca acggaattat
      601 tagtgatctg ggtcttactc tttcgcttga tgacgaatat gaaacaataa tcgataactt
      661 ccgcaagaaa gcaggcagag attatgccgc aagccgtggt gaatacttaa acggtatcat
      721 tctcgcaaat tacctaggat atgagtttat agatgcggct aaggtagtgt tcttccgtcc
      781 ggacggaaag tttgacgata ctcttacaga tacctgtctt gcttctgtat tgcacggtat
      841 ctcacacgcg gttatccccg gcttctacgg cgctaacccc gacggcactg tccgtacatt
      901 ctcaagaggc ggatctgacg taaccggttc tatcgtcgca aaggctatca atgccaagct
      961 ttatgaaaac tggacagacg taaacggctt ccttattgcc gaccccagaa tagttgacga
     1021 accaaaggtc atcgagtcta tcacatataa ggagcttcgt gagctgtcat acatgggcgc
     1081 atcggttctt cacgaagccg ctatcttccc tgtaagaagc gcaaagatac cgataaatat
     1141 ccgtaacaca aacgaccctt cggcaaaggg tacaatgata gttgcagagg ataacgagcc
     1201 tcctcagcac ataatcacag gtattgcagg taaaaagcac ttcagcgtta tctcgatcga
     1261 aaaggacgag atgaacagcg agataggctt cttacgcaga atactgacag tacttgaagc
     1321 aaacggtatg tgttttgagc atatccccac cggtatcgat acgatgagca tcatcgttga
     1381 cggtaaggac gttgacaaga ctcccgatat tgttgagaag atatgtgagg tcacagatcc
     1441 taaccatatt gagattgaga acgatttagc gcttatcgct atcgttggtc gtggtatgaa
     1501 gtcaaagaag ggtactgcca caagaatatt tgcggcatta tcacacgcag acatcaacat
     1561 caagatgata gatcagggtt caagtgagct taatatcatc gtaggtatcc atgaggctga
     1621 ctttgaaaag gctatcaagg ttatttatga tatgttcata ctcagcgaag gttaatcgct
     1681 gagttgtgaa ttgtgttttc gggttcaact taaagcacag caatcgggag agtttgaggt
     1741 ttataaaatt aaaaatcaga ctttccattc atactcagcg agggttaatc gctgagcgtt
     1801 ttaaagggcg acaattcaga gaaatatata ttttaaaagg agaagtgagc gtttatgatg
     1861 acatatgatt ctcttaccaa tgccaagctt actaagttct tcaaggcagg taactacaag
     1921 aagcagatta tcaaaaacat cgagataatc ggtcagcgta agctggtgat actcggcgac
     1981 ggacgcggtg tcaacgccga gatactcaaa
//
LOCUS       DAWAOG010000397         2002 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7366_length_2002_cov_2.019517, whole genome shotgun sequence.
ACCESSION   DAWAOG010000397 DAWAOG010000000
VERSION     DAWAOG010000397.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7366_length_2002_cov_2.019517"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          610 a          403 c          435 g          554 t
ORIGIN      
        1 aatgttctgc cgtcctcaaa gtacagcgac gggtcgatac cgccctgctc gacttcgata
       61 gggtctgacc attcgccgta gatgtcatcg gtataaacat agaagtttct gtgggtggtg
      121 tcgtttgtag tcaccatata gaacctgccg ttgttatagc ggatagtggg tgcgaataca
      181 cctcctgacg caggtatctt atcaagcatt acctgttcgg gacgtgtcag cacatagcct
      241 atctgcttcc agttcacaag gttgtcgctc tcgaatagtg caacaccggg gaaatactga
      301 aatgtgcttg ttacaagata atattttccg tttgcaaaac agacggaggg gtctgggtag
      361 aagcctttca gcacggggtt tttgtatatc atgctcaaat tcctttttaa aaagctaaaa
      421 taaatttatg cgtactttaa tagtataccg cttaaaagtg cgaatgtcaa gtaaaaagct
      481 aataaagcgg tcatgtatta gctttctaag aacgtgatat atgtaacttg cctgtgcatt
      541 cgaacaaaag tgtataaata cacaaaaaag catttattat ttgttgatta tgtgcaaatt
      601 tattagaata ccgttgaaaa attaaaacaa ttttgctatg atataaataa aggtacattt
      661 taattaaata aatggtaact attgattaca ttaatcagaa ttctgccgat tttcaaatta
      721 gctaattcaa aaggagtgat tctatgaaaa aaacaacttt gatatgcact ttaattcttg
      781 tttctctgct cagcggctgt gcgggcaata ctgtttctaa gcctgtttca tcgcaggata
      841 actctgttac gtcatctgca tcggaatcaa caaccgaaag catcacaact cccgaaacct
      901 ccgtgcctga agaatccact tcctcttatg aagacgaaaa atttactgca ataggcgtgg
      961 tggacagttc tgcagaggaa agcaaacgtg atatagcgtc atttacctac gactacggca
     1021 gtgaatataa gacgcccggc aaaaccacaa ttgacaaaat gttcagtgag gcgctcggtg
     1081 ataaaatcat tgacgatgag caggtgcaaa aagaggttgc cgatattctt gaaaagtgtg
     1141 gtaagcttga aaccgagctt tacaatgcag gtaacaagat aaaggatatg ccggatataa
     1201 ccgctgtcac aaaagacgat tacgataagt ggtttgaaag ttcggcacag ccgactgcgt
     1261 ttacgtctat gaaggaattt tatgaatcgg aaaagtcggt atattccgat attacgacat
     1321 ttgaacagtt tgaaaaggac tttgacacat tcacatatca ggacggtaaa ttctgcgtga
     1381 aaggaatttc aatcggcagg tgcaatggca cgggattgca cggtcagatt ttcggaagtt
     1441 tatcagagcg tgattgcctt tatgattatg ccaaagccta taaagacggt gataacgtaa
     1501 aattggctta tatggtgctt aattctcaga cacccggtac aagctgttat atggttttct
     1561 ccgccgacct tactaaaacc gacagcggtt acaaaatcgc ccgtaatgtt gactggagca
     1621 aggcattcgg taaaggcggc aaataactgc gtataactat tataagctat aaacaaacag
     1681 aaagcccggt acggaaaaat ccgtaccggg ctgatcttat tctgttgtgt tcggaattaa
     1741 tacattccgc ccataccgcc catgccgcct gcgggcattg cgggagcagg attttcttcc
     1801 ttcttgtcag caacaaggct ctctgttgta agaaccatag atgcaactga agcggcgttc
     1861 tgtaaagcag aacgtgttac ctttgcaggg tcaacgatac ctgcggcgat catatcaccg
     1921 tatacttcct tctgagcatc aaagccgtag cctaccttgc cttcacgacg gatagtgtcg
     1981 ataataacag agccttcaag ac
//
LOCUS       DAWAOG010000398         2000 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7375_length_2000_cov_2.051414, whole genome shotgun sequence.
ACCESSION   DAWAOG010000398 DAWAOG010000000
VERSION     DAWAOG010000398.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7375_length_2000_cov_2.051414"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1433..1442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1702..1711
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          458 a          565 c          346 g          611 t
ORIGIN      
        1 gtgctatccg acaaacagaa ctatcggtag cctgctatca ttgttaatta ctaaaatggt
       61 ataacataag tgtactatac caaaaaaaag tgagccgagc gcactatcct ttgcgtttag
      121 acacaccttt cctttttgcg ggttgcgtca cagttagctc tgtcacagtt attttaattt
      181 gcgtttaaat tttcttcaga tcctccgccc ttaccgccgc agtgatctct ccgccctgac
      241 cgataacgat atagtcctct ctatcgcccg agcctgcctg cataacctcg tacacattcg
      301 tatacacaaa cagcgcaagc gaaccgccgt tatacgtctt tgcaccgtaa ttcactttca
      361 ccttatctcc cacgacaaat ttctgtgtct gcgtatcgtc ctccgccttg taaagatccg
      421 ccgtatacag ccaccctgta ggtacgccgt caagacctat cagcgtttcc ttaccgttac
      481 cggatacctg ttgcaccgta tagaccgtgt cataaacata agaataaggc ttgaccccgt
      541 ttgtaaatac agcgccccgc tttactctta ccttatttcc ctttgaaaat ccgttgtgct
      601 tctcatcatc gggcttttcg ggaggctttt ctatgcagtg ctcctcgtac caccttgcgg
      661 ttatcgcagg ataatcaaca aagcaaatat ccccgtcaac atctttaccg ccgatattgt
      721 caatacccca ctgccacatt ttctgcccgt aatcgaatct gctcggatag tccggacttt
      781 ccgtccagtg agcaagccat atatcatact ttccgactaa ccgttctttc tgataatagc
      841 tttcaagcca tgagggattt gcataaattc ccgacggcag acccgcttca ttcaggcttt
      901 tgcagaattt caccgccata tcggttctca ctttgcttgt aagattatcg atctgcttct
      961 gctcttccat gtcaaagaac acgggatatg acggcttttc attgtccagc acctttatac
     1021 acgcttttat ctgactgtcc agttcagcgt tgtcaagcgc cgtgatatac caataacagc
     1081 cgtatgccaa cccgcgtttt acacattcga ttatattctg ccggaaatag ctgtcctcgt
     1141 ctttccctat tcccgcacgg attataacaa atttgtttcc tgccgcctgc gccgcggtaa
     1201 aatcaaattt ctcctgcgcc ctgctgatat caattccttt tattctcatg gtcgtcctcc
     1261 ccactcttgt tttttatctg ttcaagcaca ttctttatct tttgcggtac aggcagaccg
     1321 agcgccgccg cattctcaac tatcgagatc ccttcgtttg ctatatagaa aagcatcaca
     1381 gccgacatag cggcgggtat tccgccgagc acataagtgt cggttatgtg acnnnnnnnn
     1441 nncacaaatg ctagaatcag catcttcttt gcaatgcccc tgaacccgac tttactggaa
     1501 attttctttt cgctgacagc cgcaaacaca ccgctgatat agtccagcac cataaacgct
     1561 ataagcgcac ggaacagccc gttcacctca ccgtacataa atcccaggat cgccccgaca
     1621 actcccgctg cactgtcaat cgctatctgt atcttactca tatttcccct cctttttttt
     1681 tttttttttt tttttttttt tnnnnnnnnn ncgaatgcgg cggagtagca cctccgtttg
     1741 ttgcattttt cattatcatc tcatcgtccg acattcccgc cttactcaat gcggtcggct
     1801 taagctccgg atgcatctta tacaaaagct caagataagt cattccctct atatccccgg
     1861 gtatcttcat ctgttctatc cagtcataga aaccgtcctt ttcgccgtac agctggaaca
     1921 gcgcttcggt aaattcttca tcgctgtata tcttgtcgct gtactttttt ataaagtcgt
     1981 atgcatcgtc aggtgagaac
//
LOCUS       DAWAOG010000399         1989 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7447_length_1989_cov_1.678387, whole genome shotgun sequence.
ACCESSION   DAWAOG010000399 DAWAOG010000000
VERSION     DAWAOG010000399.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7447_length_1989_cov_1.678387"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1099..1108
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          503 a          499 c          369 g          608 t
ORIGIN      
        1 atgtcgataa gctctgcggc aatatcgcgt aatattgaaa cagtagttgc cctgccgttt
       61 attcttgcag tgctttttcc gtcagaatgt atatcacgct gcaataaaag ctcgtcctcg
      121 acagaaaaac cgttttctga aagcttgtcg acaacggtct gcggcagatc gtcaaaaaga
      181 cctgtaacaa ctgccttatc tgctcccgct ctgacaatat ccttacttac cctgccgccg
      241 agtattgcgt ttatgccgcc tataagtatg ctcttacctg cacctgtttc gcctgtaaat
      301 acattaagct tatcatcaaa ggcgatcgac gccttttcga taaccgcaag attttcaatt
      361 gacaattctc ttagcaaaac gccgacctcc ttactttaaa gtaattcctt aagatgacgc
      421 gtgagcattc tcgcatcact ctctgtgcgc gtcaacacga aaatcgtatc atcgcctgca
      481 atagtgccga caacatatga caaattcatc atatcaagtc cggcacacgc cgcatttgca
      541 agaccgctgt gacattttat cgaaacgatg ttcatagcat agtcgatact gactactgcc
      601 tccgaaatga tattgtgaaa ttttacggta tgtacctttt cggcctttgc gtaacaattg
      661 ataccgtttg cagatacggt tttttctatg ttcatttcgt ttatgtcacg cgatacagta
      721 gcctgcgtta cgtcgtaacc gcgttcccgg agcagtttta gaagcatctc ctgcgtttcg
      781 acttcgtttg tggatattat tctcaatatc tcagcttgtc ttttcttttt cacaatatca
      841 cccctctgtc gatcatttca gcggacgcat aagcttattg tgaacagctt tataaaaact
      901 gcttccgttt atatcgatca gtgacagctc atgttttgag cgtcttatct ttatcgtttc
      961 gccctcgttg aagtccatat catcgttgcc gtctatactt agtgaaacac cgctgttttc
     1021 atacgcggaa aaacgtaccg ttatctcaga gtcggaagaa aagatcatcg gtcttccgaa
     1081 tagcgaatgc gcgcacagnn nnnnnnnnta taccgtctgc ccttatcttt gtgacctcgt
     1141 actcttcgac cgatacaata aattcaggaa gctttgaata ccgacacttg ctgaacacaa
     1201 cgtcatttat ggcactgaat tttcctcttc cctcatactc tatatcaagc agcattcttc
     1261 tgcttatgct gtaatcgccg gttctgagcc gttcaagcgt ttcaagctca tcgctttcta
     1321 tagaagtcat aaagccaagc ctgcctgtat ttattccaag aaggggtttt ccgcacattg
     1381 cggctttctg cccccatttc agaatagtac cgtcaccgcc tgcgatagga gaagcgataa
     1441 cctcaccgtg ttcaaaaccg agtgttgtca caaattcagc aatttcttca cagacctttt
     1501 tttgtacttt gatatcacgc acaataccgt ttttgctgag atgcgctttt cccgcttcaa
     1561 actgcggctt tataagtgcc acgcactcgc cgccatcctt aagtagtgca aatgcagtcg
     1621 gcaaaacaag cttaagtgat ataaaggata catctatact tataaagtcg acctgcggca
     1681 atatgccgac acagtttctt atatcggtct tttccataga aattactcgg ctgtcgttcc
     1741 tgagcttttc atcaagctgg tcagtaccga catctacgga ataaaccttt tcagcgccgt
     1801 ttttgagcat acaatcggta aatccgcctg tagatgcacc tatatcaatg cagacagcgc
     1861 catcaagatg cagtctgtaa tgagataatg ctttttctaa tttgagtccg cccctgccga
     1921 catatttagg cagttcaccg atgattttta cggtatcatc gtcatttaca gtaaacgacg
     1981 gctttgagc
//
LOCUS       DAWAOG010000400         1988 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7455_length_1988_cov_1.989136, whole genome shotgun sequence.
ACCESSION   DAWAOG010000400 DAWAOG010000000
VERSION     DAWAOG010000400.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7455_length_1988_cov_1.989136"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    404..413
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    935..944
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          625 a          409 c          490 g          444 t
ORIGIN      
        1 gtacggcttt tatgcttctc ggctttgccg gaatgctgca acttatgata agacctaata
       61 taataaacgt gtgcgaatac aattcaaagg cggttacggt gagccttata gacgatgcga
      121 taaacgagcg gctttcggag cttggagaag atgtcggata cagcgctctt gtaaagctca
      181 gttacaccgc agatggcaga gtttcttcta tcgaaagcaa cactaagctg ataaaccgca
      241 ttaagaacga tatgctgacc gagataaacg acaggctcat gaaaggtgaa accgaaaacg
      301 ttgatcttac tgtcggtacg ctcagcggaa taccgctttt tcacggctca ggtcctaccg
      361 tcagaatgaa ggtagaacca aagggctatg ccgatgcagt attnnnnnnn nnnatttata
      421 agtgaattta ccgatgcagg acttaaccag acgcttcaca gaatgataat gcgtactact
      481 gtcagcgtta ccgcatttat acctatgtac tcggtggaaa caaaggtcag cggagatttt
      541 cttattgccg aaacggtcat tgtcggaaat gtgccggaaa gcttcactca tgtggtatcc
      601 gaggataaag acattgtcga tgcaataaat gactttgagg cagagccgta tgaataaccg
      661 aaaacggaag gaattacaat ggataaagag cagataaaaa gtgaatacga acagctttgc
      721 aagcaagccg aacagcacaa tttcaattac tacgttctcg acaatccgac tgtagaagat
      781 gatgagtacg acaggctgat gagaagaata aaggagatag aagcggaaaa tcccgaaatt
      841 gtttccgaaa gttctcccac acagcacgtc ggcggatatg ccatcaacac atttgagaag
      901 gtaactcacg aggtgcagat gggcagctta caggnnnnnn nnnnaaaaaa agctgtaggc
      961 aaggctgtat actgcgttga acccaagata gacggcttat ctgtttcact tgaatacaag
     1021 gacggaatat tcacaagagg ctctacacga ggcgacggct ttgtgggcga ggacatcact
     1081 aaaaatctga aaaccataaa gtcgattcct ttggagctta acgagaagct cccctttata
     1141 gaagtcagag gagaggtcta tatgcccaaa gccgactttg aaaggctggt aaaaacacag
     1201 cttgaaaacg atgaacagcc ggctaaaaat ccgcgtaatg ccgccgcagg ctcactgcgt
     1261 cagaaagaca gcagaataac tgcatcaaga ggacttgata tattcgtatt caatcttcag
     1321 cgtattgagg gtaaggagct tacctcacac agcgagagcc ttgactatat gaagaagctc
     1381 ggcttcaatg cgatagacgg ctataagacc tttgataata tcgaagacgc cgtttcgcgc
     1441 attatggaga taggcgagaa cagacaaagc tattcctatg atatagacgg tgcggtcata
     1501 aaggtcgata attttgaact aagaaacgag ctcggcagta ccgccaaagt gccgaagtgg
     1561 gcggtagctt tcaagtatcc tcccgaagaa aaggaaacga aactgattga tatagagata
     1621 aatgtcggca gaaccggagc acttactccc gttgccgtat ttgagcctgt atggcttgca
     1681 ggtactaccg tatctagagc agtgcttcat aatcaggact atatcgacag caaggatata
     1741 agaatcggcg atattatagc agtacgcaag gcaggagata ttatccctga ggtcgtgcgc
     1801 agtgtcagcc acgccgaaaa cagtgtgccg tttacgatac cgcatatatg ccctgtctgc
     1861 ggcggaaaag ccgaaagagc ggaggacgag gcggtaattc gctgtgtaaa cataaattgt
     1921 ccggcgcagc ttctcaagaa cattgaacat tttgcatcaa gaacggctat gaacattgac
     1981 ggactggg
//
LOCUS       DAWAOG010000401         1987 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7465_length_1987_cov_2.143375, whole genome shotgun sequence.
ACCESSION   DAWAOG010000401 DAWAOG010000000
VERSION     DAWAOG010000401.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7465_length_1987_cov_2.143375"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          511 a          504 c          373 g          599 t
ORIGIN      
        1 cagtatatca cgccctcaac gggtaagaac atcataatgt cgctctgata tgcaatgcat
       61 tcacggcagg cgcgcaggtc aggcataaag cctatccttg acagaaacct gaactcaaat
      121 atagctttta taaatccgag cggcagcttc tgcttttcaa gctggaagta cgtcatacag
      181 gtaaatctga gcaagtcatc gtgctcttct tccgaggtaa cacagtactt tattatctcg
      241 gcaaaatatg ccgcaagcga cagggcttct atacttgatg aaatgccgta aaagctgcat
      301 atcggctcgg cactgttgag cgtatagccc ttactgctac ggctcacaca gaaagttgcg
      361 taactgaaaa gcgatgccgc ccccgaattt ttaccgccta tcttttttgc accgtgcgcc
      421 ataacctcta tcattccgag cttgtccgta aacaggtcaa gaaaacagct gttctcgcct
      481 atactgcgct gtccgataac aagccctttc agcgtaataa gcattgaaca gctactcctt
      541 taaatatctc agtcagcctt aagtccgaag tcggcaatgt cagactctct gtttctccag
      601 tcttccttta cctttaccca tatctgcata ttgaccttac agccgaaata gtcctgcata
      661 tcttctcttg cctcagaccc tatctttttg agcattgagc cctgctttcc gattatcata
      721 cccttgtgtg aagccttttc gcagacgatc acaatgccga tatcgactat ttccttaccg
      781 ttaggctttt gtcttacttc catagtttcg acatatacgg cagttccgtg cgggatctcc
      841 tcgttcgtat tgcggagtat cttttcacgg acgatctcag caagccaaac cttttcagcc
      901 tggtcagtcg gaagctcatc gtcaaaataa tgcggcgaca gctttacata tctgtcgata
      961 atgggcatta tttcttcaac gcctattcgc tgtcttacgc ttatcggaac gatctccttg
     1021 aaatcgtaca gctcgctgta ttccttcata agcgaaagaa gctcggactt gtcctttatt
     1081 aaatcgacct tgtttataag cagaataaca tctgtctttc tgtccttgaa gcggtcgata
     1141 agattatgct ctataggcga gatcttctta gtagcgtcag ccatcataag tataacatca
     1201 acatccgtaa cgctctctct tatggatttg agcatctgat cggaaagctt attctttgcc
     1261 ttgtgcatac cgggcgtatc tatgaacaca tactgcgttt cgccctttgt gagtacaccg
     1321 tttattcttg ttctggtagt ctgcggctta tcgctgacta tcgctatctt ttcaccgaca
     1381 agataatttg tgagagtaga ctttcccgca ttaggtctgc ctgctatagc cacaaatact
     1441 gatttaggat tgttcattta ttcatctttc ctttgatatt tcttttcttt ccttttgcaa
     1501 caaacataaa tgcaaggcag gcaaaagccg ccagcatagc caaattccat atatcacccg
     1561 taaaaaatct gaatataagt acaaattttt ccgtgtccca aaacagtgtt atgccgatta
     1621 tgaccgcggc tatagccgtg acaaacaccg cacctgcggc tacgtcctta ccgactttgg
     1681 cgagcgccga gtatcctgga gaaaccttat ctatcacgac ttctattgca gtattcatca
     1741 tttcagccga tattacaaca acgcaggtga ttatcagcaa tatgaactgc tctttcgtga
     1801 agttgtaaaa ctgagaaaga taaagcacac acatcgttgc cacaatatgg atgcgcatat
     1861 tcatctcatg acttacgcag aaacgtatgc cgcgtgccgc atatttaaag ctgttaccta
     1921 gtcttttaaa cactttatcc tccgggtcat tcccgtgtga tcttaaggtt gttcagcaca
     1981 cgatcct
//
LOCUS       DAWAOG010000402         1987 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7463_length_1987_cov_2.211698, whole genome shotgun sequence.
ACCESSION   DAWAOG010000402 DAWAOG010000000
VERSION     DAWAOG010000402.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7463_length_1987_cov_2.211698"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          569 a          419 c          511 g          488 t
ORIGIN      
        1 gtcgtgtaca gggcaagaga gtgggctata acgagatata tgaagctatc ggtcagtatt
       61 caaacggcac tataggcgat gatgttcttc ttgactatga gaacaacgct tgccctacct
      121 gcggctcatg ttctggtatg tatacggcaa attcaatgaa ctgccttaca gaagcaatcg
      181 gtctttcgat gccgaagtcg ggtacagagc ctgctgtaaa tgccgctcgc cgcagacttg
      241 ctaaggaaac gggcgaaaga atagtagagc ttgtaaagca gaacatctgc gctaaggata
      301 taatcacaaa gaagaatatg atgaacgcac tcgctaccga tatggcgatc ggcgcttctt
      361 caaatactgt acttcatctt cttgctatcg cgcacgaggc aggagttgac atttctcttg
      421 acgatatcga caacaagagc aagacaacac ctcagctgtc aaagctcaac cccgcaagcg
      481 atatatttat aacagatctt aatgatgtcg gcggtatcca gtcggttatc aaggagcttg
      541 caaagggcgg tcatgttgat acaagcgttc ttacggtatc cggtacacag gcagacagaa
      601 ttgcaaaagc tccgaatgca gacggtacaa taatccacac ttgtgaaaat cctatcagaa
      661 aagacggcgg tcttgcaata ctcagcggta acctcgctga aaacggctct gttgtaaagc
      721 agggtgctgt caagcccgaa atgatggact tcacaggtac tgcaaaggta tttgacggcg
      781 aacaggcagc ttgcgatgct attctcaata acgttatcat acccggtgac gttgtagtta
      841 tccgctatga aggacctaag ggaggtcccg gtatgcccga gatgctcgcg cccactgcgg
      901 ctattgtagg taagggactt gacggaagtg tagcgcttat cacagacgga cgtttcagcg
      961 gtgccagcag aggtgcggct gtaggtcacg tttctcccga ggcggctgac ggcggactta
     1021 tcgcttacgt tgaaaacgga gataagatcc atatcaatat ccccgcaaga agcattgagc
     1081 ttctcgttga cgatgaaaca atagcacagc gtaaggctaa gggtgtaaat gctcccgtaa
     1141 gacagcttga cggttatctc aagcgttacg caaagctggt tacaagctcg aatacaggcg
     1201 ctgtatttga agattgatat gctaatttaa taccgtttat caaaggctgt gtatttaggt
     1261 acacagcctt gcggtgatat tatcgacaga aaggagacgg tagctatcgc ttataaggat
     1321 aatatgcacg caggtcacag ggaacgtatg ataaaccgcc ttaaagaaca gggcggtgaa
     1381 agctttcagt cgcatgagct tcttgaaatg atgctttaca gctcattcag acagtgcgat
     1441 accaacccta tagctcacag acttatcgac agatttggaa cgctcagcgg tgtgttctcg
     1501 gcaagcgaaa ccgaactttg tgaagttgag ggtgtaggca gatctaccgc tcagatgata
     1561 atcgtactgc gtgctaatat agaacgtatg ctgtctgaga gtgttcagaa aggtgaaatg
     1621 ctcggagcat cgggagcagt caaggattat tgcacggggt tgttccgctt tgtcgatacc
     1681 gaacagctca gaatgatatt tctcgacagt gagtattgct ttgtgtcaca ggaaataata
     1741 agcacaggag cgcttgaaca ggtgcgtccg gatgtaatga gattgcttga aaaggctgtc
     1801 aggaaacgat gccctatagt cgttatggct cataatcatc cgagaggcag tgaaatgccg
     1861 tctactgagg ataagataat gacaagaaac cttttcaact tacttgaaaa ggcaggagta
     1921 tatcttgcag atcatattat agtaggtatg cacggcagta tttctatgcg tgagaacggt
     1981 attcttc
//
LOCUS       DAWAOG010000403         1986 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7471_length_1986_cov_2.361989, whole genome shotgun sequence.
ACCESSION   DAWAOG010000403 DAWAOG010000000
VERSION     DAWAOG010000403.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7471_length_1986_cov_2.361989"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    842..851
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          507 a          476 c          366 g          627 t
ORIGIN      
        1 tgtatacaca atgtatctgt tcagtatccg cccggaatat ttttcggtaa atacatcaag
       61 cgatctatgc gttttataac ccgatgattt tacctctatc ggtactatct tgtttttcct
      121 tgcaagcagg aaatcaacct cataattatg ccttgtttct tcattcataa ttgtatgata
      181 gaataattcg tcaccgttac ttgcaataca ctgtgcgacc gcattttcat ataaatatcc
      241 gagattgacg ctcagcttat cactgaggac tttctgatat atatcatttt cagtaaaatc
      301 cttatccttg aacataagcg ttacaaacag ccccgtgtca ctaaggaaca gtttaaaacg
      361 ggataaatcc ttagtgcttg acattccggt gttggggtca tttacattat acgaaacaag
      421 gacagtcttt gaatccttaa gctcggcaat aagctccaaa attttgtccg attgctcttt
      481 accgattact gtgcccggct gaaatctggc tacattctta ttaagctgtg ccggtattgc
      541 atcaaacagc atcgagagcc tgcccgtctg atctattttt gcaaaatctg cctcataaag
      601 atttagtata tcacgcttaa tttcatcaac ttttctgaaa ttattgcttt caacataagc
      661 actcaccgcc tgcggcattc cacctacaag catatatagt ctgaactgcc ttagctgttt
      721 acggtgtact gcgtccccga gcggaatatt tttttcaaag cattgtctta gtaaagaaaa
      781 tgtcgtttca tcaccgagtg cccacagaaa ttcctcatag tccatagggt acatactgac
      841 tnnnnnnnnn nagtcatatc tgtgatcttt gacaagcgtt tttatagcct gccgtgccat
      901 aggacatagc tggacttcgt caaatatgat aaccgacctt cgttcgtgca gatccttcct
      961 gtactgaagc tgaagctgca aaaaaagata attcagatcg gaaacatcgt caaacagctc
     1021 tctgacctct ttcgacgctg tagcaaaatc aacaagtata tagctttcat attcgttctt
     1081 cgcaaactcc tcaacgactg tagattttcc gatacgcctt gcaccctcta caagcagagc
     1141 agtattgccg cccgattcat ttttccactg taacattttg tcgtatatct tccgcttgaa
     1201 cataggcttt ctcctttttc gcatttttta ttttgctgat ttcaagaata atcgcatttt
     1261 ttataaaagc gtttttatgc tttatcgcaa ttttcatttc cggtatttta tccgttatcg
     1321 caatttttat acttcaatat taccactttc ccgcactttt tgcaataccc atccccattt
     1381 tttgcataaa aaccccgccc ccgaaaactc gggggcgggg aaaataccta tatctatcgc
     1441 ttcacttacg cattcttgtc aagacctgtg atttcaagtg tatcacgggc gataagaagc
     1501 tcctcgtttg taggaattac ccatacctcg accttgctgt cgggagcaga gatctttgcg
     1561 agcttgccct ttaagccgtc gtttacagag ttgtcgagct tgatgccgag atattccata
     1621 ttctcgcata cgccctttct tacttccgga gcgttctcgc cgataccgcc tgtgaagata
     1681 acaacgtcaa gaccgttcat agcggctgtg taagaaccga tgtacttctt tatctcgtat
     1741 gtcagcatat ctgtaacaag ctgtgaacgc ttgtcgccgt gctgtgcggc acttgtgata
     1801 tctctgttgt cgctgaactg acccgaaagt ccgaggaagc ccgacttctt gttgagatag
     1861 tcgctcatct gtgcgggagt gaagcccagc ttgttctgta cgaattcaac agccgagggg
     1921 tcaacgctgc ccgagcgtgt acccatgatg ataccgtcaa gaggagtaaa gcccattgat
     1981 gtatct
//
LOCUS       DAWAOG010000404         1982 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7486_length_1982_cov_1.453555, whole genome shotgun sequence.
ACCESSION   DAWAOG010000404 DAWAOG010000000
VERSION     DAWAOG010000404.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7486_length_1982_cov_1.453555"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          612 a          333 c          443 g          594 t
ORIGIN      
        1 ccgacggctg aggggagaac tgaaaaaaca gtattttgat ttttacacaa gggaggcaac
       61 agaaaaatga cacgacagct tgtggcgtta acacttacag tcctgatgca tattgtttgc
      121 tttgctatta tgaccgaacg gaaatattct gtaaaaaaaa cagtttcaat ttatttcgca
      181 tttttaaccg tctttgtctg ttttgcaatg ctcgcttcac gggtattgtt tgacattcat
      241 tctttttatg ctacttctct tacgttttta agtacaatgc ttgtgtcgtt tattattttc
      301 atggtgacgt cggcagaccc tgcctgtaaa aaggtgtttt tgtttcttag ctattcgtcg
      361 atgttctgca tttttttctg cagttcagct atgataagca gcgtctggtt taaagacggc
      421 ttcggtatcg ctgccgtata tgtgaaaata atcataagaa ccctgctgta tctgccggta
      481 atatggtcat atatcgtctt tttgcgtccg gcgatacgtg aggtttcggg ttcaaataaa
      541 aaaatctggc actctatttc gctggtttct gtacagtttt taagtgtatt ttctatattt
      601 tgcttcattt atactatgaa aaatgacttc agggcatggt atagtctttt gtttgcagcc
      661 actgttctga tttatttttc ggtattgttg attagttttg gtatgatccg ttatatgata
      721 aaggaaagtc gaatggagtt gattgaagaa aacatgaagc atctgcaaga ccggcttaaa
      781 actgcaaaag aaaacgagat gtctgccaaa accgtccggc atgatttccg acatcacaac
      841 caaaatattg ccacaatgat ccaaaaggga gaaaccaatg aagctctgca ctacatagaa
      901 cagtacaatg aaagtctgga cgctgcaaga acgaaagaat tttgttccca tgtgacggta
      961 aatgcaatac ttagcagtct ttatatcaaa gcgcaaaatg acggaatttg tatttcaatt
     1021 gaagcggaca cgcaggaagg taccaacatt agtgatatgg attttgtcgc tatactctca
     1081 aatattttgg aaaacgctgt caatggctgc aaggaatgtg gtgctcatgg taagataaat
     1141 gtcaacatcc gaactgtttc ggataaaacg gttattgtgt gtagcaactc ctgtaaaccg
     1201 gggcttgtta tcgaaaataa tatgataaag cataggggaa tcggcattga gagtatgctg
     1261 acagcggcga gaaaatacga tggcgatata agttatcgtt tagagaatga tgtactcacc
     1321 ctatgtgtta ttctgaaaaa atgattttga aaaccaattg cgttcaaaaa acgaccaatt
     1381 acgaagaaaa ccatccgtaa ggatggtttt ttgttatact ttattaaatt taatgtgtca
     1441 ggaggttaga atacgagcgt ttttagtaag aagaagctga cctttggcaa ggcggtaaaa
     1501 tttaattatg aacgagggta tgaaaaactg aaaacagatc aaaaactctt aaaaacatat
     1561 aaacagacag gcttatggga aggggaatgg aacgaattat gaaggataag aaaaagagaa
     1621 tagtaaaacg cctggcggcg gtgaccttga gtcttattat tgtgctttct cagattggtg
     1681 gtacagcacc atggaacagc ggatttgctg tccgtggggc ggaggtatct tcacctatag
     1741 ttttgaaaag aatagacggc agcggagaag ccttcattta ccacagcttt gtggaaacaa
     1801 agtcctttac gacaggtcag acttatgcat ttttaaacgg cattttcaat tatgacgatg
     1861 gtgtgtccgc gcttggagca ggagcaatct taaaggcgac ggatgtgaca aacagatccg
     1921 cccactacat aaaaaacaag tatggaacaa atgccaaggt aaaggtttat gtggacgtag
     1981 tt
//
LOCUS       DAWAOG010000405         1973 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7523_length_1973_cov_2.120438, whole genome shotgun sequence.
ACCESSION   DAWAOG010000405 DAWAOG010000000
VERSION     DAWAOG010000405.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7523_length_1973_cov_2.120438"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          563 a          410 c          493 g          507 t
ORIGIN      
        1 gtgcagacct gctgaacccg ggagatgttg cagtgcttgt agtacctatc gacaaagcag
       61 ctccgaaagg caggcttata cttcctcagc aacagacaat aagggatata cttgaagctg
      121 atgctacggc tgttgtatgt aaggaacatg agctgcgtga tacgctgtca aagctaaata
      181 taaagcctgc gctggtgata accgacagtc aggttttctc aaaggtttcg gcagatgtcc
      241 ccgatgatat aatgctgaca tcattttcga tacttatggc gcgttacaag ggcgatcttg
      301 aaacaaatgt tctcggagtt accgcacttg acaggttgaa tgacggtgat aaggtactta
      361 tatcggaggg ttgtactcat cacaggcagt gtgacgatat aggtacggtt aaaattccga
      421 gatgggtaag caattacacc ggaaagcaga tacagttcga gttctcatcg ggcggcactt
      481 tccccgaaga tctaagccca tataagctga ttattcattg cggaggctgc acccttacag
      541 agcgtgagat gaagtaccgc atagcctgtg ctaaagacgc gggtgtgccg ataaccaatt
      601 acggcatctg tattgcctac actcagggca tacttaagcg cagtgtacag ccgttcgagt
      661 acatttattc acttctttgc ggagataacg atgagaactg aaagcgattt tctcggcagt
      721 gtcaaactgc ccgatgactg cctttacgga ataaataccg caagggcggt atcaaacttc
      781 agtatatccg gtaaggctgt tcataaagag ctgatatatg agcttattat gctgaaaaga
      841 tccgccgctg tcgcaaaccg tgacgcgggg cttcttgatg ataatatagc acagtacatt
      901 gtaagtgcgt gcgataagct cagctttaaa attgatatgt cgctttttcc gactgacaca
      961 ttacagggcg gagcgggtac ttctataaat atgaatgtaa acgaggtcgt ctgcaacacc
     1021 gctttgctta ttgccggcag aaggtgcggt gattacgact ttatagaccc gcttgaccat
     1081 gttaatctca atcagtccac aaatgatatc ttcccgactg ctgtacgcat tgcttcaata
     1141 aagcttatac gcaagctgag tgatgggtgt gcacagcttc agactgcgct tcaggcaaaa
     1201 gaacaggagc ttgcgcacat aaaaaagatc ggcagaaccg agcttatgaa tgcaacagaa
     1261 atcactctcg gagagcagtt cggatcatat gcacagtgta tagcaaggga cagatggcgg
     1321 atatataagg ctgaagaacg tctgcgattt atcaatatcg gaggtgcggc ggtaggcaaa
     1381 gcggcgaata ataaatatgt tttccgtatg acggagctta tccgcaagga aagcggtata
     1441 ggtcttgccc gtgccgaata tcctatggat atcacgcaga attgtgatgt gtttgcagaa
     1501 gtgtcgggac tgctgaaatc tcttgcggtt gatctgataa agattgcaaa tgacctgcgg
     1561 cttatgaacg ccgaatttat cggtgaggta aagctttctc ctatgcagaa gggaagcacg
     1621 gcaatgccgg gtaaagtgaa ccctgttata cctgaagccg taatacaggc ggcagttaaa
     1681 gcgatgtcaa atgatactgc tgttacatat gcttcatcta tgggaaattt tgagctgaat
     1741 gcatttatgc cgcttattgc cgacagcttt attgacagcc ttaaaattct taccggtgca
     1801 gtcttgactt ttaccgaaaa atgcgttaaa attctcacag ccgataaaaa tgcctgcggc
     1861 tatcacctgg aacacagcag agcgtatgct atgaaatatg ttcctgtact ggggtacgaa
     1921 gcagtagaaa agctccttgt cgagtgtgaa aataacaaag agcgtttcat agc
//
LOCUS       DAWAOG010000406         1973 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7522_length_1973_cov_2.251303, whole genome shotgun sequence.
ACCESSION   DAWAOG010000406 DAWAOG010000000
VERSION     DAWAOG010000406.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7522_length_1973_cov_2.251303"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          529 a          507 c          436 g          501 t
ORIGIN      
        1 cggggaggat aaacagcgtc ataggctcaa taaattcagg cattatcgta atgccgaacc
       61 actgtcccga gccgaatatc tcacggacag agcctacaag cacaagcgtc agcgtaaatc
      121 ccagtcccat tccaagtccg tccattattg acggaattac cttgttttta gatgcgaaca
      181 tctccgctct gccgagaatg atacagttta cggtaataag actcaggtac ataccaaggc
      241 ttgaatacac atcgggggca aacgcctgga gcataaggct tacaaacgtt acaaatcccg
      301 ctactataac tatatatgca ggaagcctta ccgtatcggg gataactttt ttaagcagtg
      361 aaattactat gtttgaacat acaagtacaa acgttgtgga aagtcccatt cctacaccgt
      421 ttgccgccat tgtggtaatg gcgagtgtcg gacacatacc gagaagcatt accatattcg
      481 ggttctcttt tatgagtcct ttggtaaact ctttccagtt actcattcgg ctgtacctcc
      541 ttatccatca ccgagtatgc ctttacggca agatttacgg cttttgccat accctttgag
      601 gaaagcgtcg agcctgtaat tccctgtacc tcgttttcgt ttttcggttc tgacttgact
      661 atcgatatat ctgacttaac gccgtagaac ctctgcagga atgcgtcatc gtttatcttt
      721 gagccaagac ccggagtttc gccgagtgcg ataatactga taccgcttac cgcaccgttt
      781 tcatcaatcg ctatcacagc gtctatgccg tccgccgcat atccgtcaac aacggtttca
      841 aaaaggaacg tgccttttgt cttgttcttt actatcttta cgatattatc aaactcttca
      901 ccatccagct ttgccgccgc cttatcggtg ataagcgcat attcgccctc gccgtctgcg
      961 gcgatacagc ctgctttcag gtcatccgta agaacaccgg acgtatcgac ataagtcaga
     1021 ttatatgtaa ctatcagcag tgcgcagata agcgtacaga tgaccgacag tacaagaacc
     1081 ggcttgattt tactaagtac actcatttct taccgccttt ctgctcactt gccgcaccga
     1141 atgccttcgg cactgtgaag cggtcgataa gcggagagag gatattcata agcagtatcg
     1201 agtacgatac gccctcgggc atactgccaa agcttcgtat gagcatagtc acaagtccgc
     1261 agccgatgcc gaatattatc ctgccctttg tggtaatagg agtagtcgca tagtcggtag
     1321 ccataaatat agcgccaagc agaagtccgc ccgaaagaac agacgctagc gcaccctcaa
     1381 ggcttccgct tgagaatatc gacagtacga acaccgtacc gataaagcac agcggagtta
     1441 cgggcgatat tacacggaca gccactaaga aaattccgcc tataagcagt gccagagcgc
     1501 aggtttcgcc tatacagccg cctgttgttc cgaaaaacag gtcggcaagc gaagcgggag
     1561 tttcgctgac aaggggtgtc gatgtagtta ctatctctcc tgcgttacgg tagtagaacg
     1621 gctctgccca tgtcgtcata taagacgtga acgacagcat aagcactatt ctgcctgtta
     1681 tcgcggggtt agcaaagttc tgaccgatac cgccgaagaa ctgctttact attactattg
     1741 ccacaaagca accgattatt gccatataaa aaggcaatgt gacgggcagg ttgaatgcaa
     1801 ggagcatacc tgttaccaca gcggacagat cgcttatcgt gttgcttctt ttcattatct
     1861 ttctgcaagc ccactcaaat ataacgcagg acgtgcagca tattactgtc attatgagcg
     1921 ctctgagccc gaagatccac acagacgcaa caagcgcggg gagcagtgct ata
//
LOCUS       DAWAOG010000407         1972 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7526_length_1972_cov_2.362546, whole genome shotgun sequence.
ACCESSION   DAWAOG010000407 DAWAOG010000000
VERSION     DAWAOG010000407.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7526_length_1972_cov_2.362546"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          429 a          503 c          444 g          596 t
ORIGIN      
        1 gctttgcctt acccgaaagc gccgccgaca gcgtttctat ccttctgtgc tcatattcct
       61 gtgactgcgc ctcggtatca gtaagcgtca gatctgaagc gggaaactga tatgcggaca
      121 tatccccgca cattgtgttt atatcctcgg tgagctttgc ggctgacgct tcgctctcgg
      181 ttatgacaag tatcggggag ggtatctttt catagctcag tgacgctaaa aaatgcgcct
      241 tgtgtatatg cgaaagtccc gtcaccaaca aggggacggg tttatcgctc aaaatatgat
      301 cgactgactt tttatactcg gtcagtgtgt cagccatatt tttaagaagt tccatattgc
      361 ctttctttct tacgagttgt acttgttcat cgccatatct atcttgccgg caacaataag
      421 ctttgcggct tcggcagcat tgtcaagcgc cttttccagg ttctcgccgt cttccttttt
      481 gaagcccgac agtacccagt ccgcaagcgg aaaatcaggg tgcggctttg caccgacacc
      541 tatctttacg cggggaaaag cgtcactgtt tgtgagcagt ataatgctct ttatgccgtt
      601 atgaccgccg tcactgcctt ttctgcgtat ccttgtcctg ccgacatcaa gcgagatatc
      661 atcgtacatt acaagtatat tctgaggtgc gagcttatag aagctcatag cctcacttac
      721 cgcttcgccg ctgttgttca taaaggttga cggcttcatt aaaaggcagt gcttgccctc
      781 tatcatgcag tccgctatca gcgacttgaa tttcagcttt tttatctgca cgccgagctt
      841 ttcggcaagt gtatctaccg ccataaagcc tgcgttgtgg cgcgtgttct catactgtgt
      901 accggggttt ccgagtccca ctatgagata ttcgacagcg ccctgcggtg caggcgctct
      961 gcctgcggct atcttatcaa atatatcgga taatgacatt tttatttgtc ctttgctttt
     1021 gctgttgtca cagattttct gaggtaagcc gttacaagtc tgctcatcat tcttgcagcc
     1081 tttttacttg caggcttttt cattctccac gtccagtttc ctccgacagt cgatggaacg
     1141 ttcatacgtc ccctgccgtc aatgccgagg atatcctgca tgggtattat cgcaagggcg
     1201 gcgggacttg agtaaacgct tcttataaag cttacattct tgtgctccaa gaagtttgcg
     1261 tttatgtagc tctttgccat ctttgccgcc ttagagtctg cgaatctgag ccagcccatt
     1321 atagtatcgt tgtcgtgtgt tcctgtatac gcaacgcaat tattcacata gttgtggcac
     1381 aggtgcgtat tgtcctgata ttcgttattg aagccgaact gtaaaactct cataccgggg
     1441 aagccggtat cgctgagaag ctttttaacg ctgtcgaata tatcgcctaa atcctctgct
     1501 atgatattta catcgccgag ctgttctttc accgtgttaa acagcttcat accggggccc
     1561 tgcttccact caccgtattc cgccgttgtt gcgtccgcgg gtatagcata gtaagtatca
     1621 aatgcgcgga agtggtctat tcttaccata tcgaacagct tcatagcgtg gtctattctc
     1681 gcgatccacc agctgtagcc gtcacgctcc atagcatccc agttgtacag cggattgccc
     1741 cacagctgtc ctgtaggcga gaaataatcg ggtggtactc ccgcaacaag agtaggtctg
     1801 agcgagatat ccatagtgaa cagctgagga tctacccata catcggcgct gtccatcgcc
     1861 gcatatatcg gaatgtcgcc tattattgaa ataccattct tgttcgcata tgctttcagc
     1921 ttgttccact gggtaaagaa catatactgt acaaagcggt ggaactttat ct
//
LOCUS       DAWAOG010000408         1971 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7537_length_1971_cov_2.337161, whole genome shotgun sequence.
ACCESSION   DAWAOG010000408 DAWAOG010000000
VERSION     DAWAOG010000408.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7537_length_1971_cov_2.337161"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    642..651
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          598 a          380 c          504 g          479 t
ORIGIN      
        1 aaaacgcacc gaataagtct atgtttgaac agataaagac cgctcttgct acctgcaccg
       61 atgagcagaa gaaacaacag cttgaaacac tgttatcatc gcctgcatac gcccacagaa
      121 tagggcgctt aaatgatctt gacagcaaga taagtgatat gtgctcacgc cttgcaaacg
      181 ccgaaatagg cgttgataca gcgcatctgg gcgatataat tcagagtgcg tatatgcaga
      241 cggttttcga tgtgacgaag ggtgcggatt accgtgcggc ttttgattta attcccgaaa
      301 gccgtgtgaa agctattctg tctactaact ggagcggtca gatgttctcc cagcgtgtct
      361 gggataacac aaacgcactt gcagacgggc tgaagcacga tatgcttgtg ggcattatgg
      421 caggaaagtc cgagcagcac atggcggacg atataatgag ccgatgcggt gtcggcgctt
      481 tcgaggcacg caggcttgtc cggacggaaa ccacctgcgt tgcgaatatg gcggagctgt
      541 acggctacaa ggagcttgac attgacgaat acgagttttc cgcctgcctt gacagccgca
      601 caagcgatct atgccgtgag cttgacggta aggtgttcaa gnnnnnnnnn ntaaatcttc
      661 cgcctatgcg cccgttctgc cgttctacaa cgctccctgt tctgccgagc gaggaggatc
      721 ttgataaaga gctcgccgaa ctgggcgatg agataggcgc agatgttgac tttgacgagt
      781 gggagcggaa cttacagcag ggcgaggacg gcaagtggcg gtatgttgca ggaagtgcgg
      841 gtaaagtcaa tgcggataaa ccaatgaggt ttgcaggaaa tggtgttgac aaatcaaagg
      901 aaaatggtat aattaatata gaatttgatg aatttgtacc gtgtttaaaa gatgcagaaa
      961 ccggtgagat ttttcagacg gaagttagag aactcacgaa atctgaatat tcacgcttca
     1021 aggaggctga tggttggaat atagattggt cagatattcc tgatggtgaa caggtcttag
     1081 gagtatatct taaaaacgat acagaaccac aggggcttat agctatacgc aaagataaag
     1141 gcggtgtata cttgtcgtat gcaagtacag cacctatcaa caacaaactg cttaacaatg
     1201 gaaaacagaa gtttattgga gtcggaggac atttatttgc ggcggctata gaggagtccg
     1261 taaaagcggg aaatgagacc ggatgtatat acggatatgc ggctaatcaa gaagttctta
     1321 atcattatat agagaatttt ggcgctgttc acttgccaat cactcatgaa tatcagttta
     1381 taatagacgg ggaagcggca gagcaaattc tctcaaaata taattttgaa aggatataaa
     1441 aatgactgaa ataataccgg atcctgatat taaggaattt gatggggtat ttgtaggagg
     1501 cgaaaagcgt ttgcccccta atgaatataa tcttagccga ttggcagaat acttacggaa
     1561 gacgaataag aaattttcag atttgagcaa agaagagaaa aaagcattta ttttgcctta
     1621 aaaccgccca cagccgtgag cggttttatt ctatccgttt ttttaaaaat taaatgcgtt
     1681 tttaaatacc aacttcacaa aaacagccgt tttttttgtg aagttcggcg cagatcagaa
     1741 ccaaacttaa taattatacc gccccttttg gagcggtatt tttataccca aaaacaattt
     1801 attccgaacg ttgtgggcaa tgaacacggt gggcggagaa aggacagaaa catgaacaac
     1861 agaagaattt tcatcggctt acagcacttc gcagagggcg agggggacgg tggtacaagc
     1921 gcaaacgttc ccggcaatca gactgccgat aacggcggtg acgcccagga t
//
LOCUS       DAWAOG010000409         1970 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7545_length_1970_cov_2.279373, whole genome shotgun sequence.
ACCESSION   DAWAOG010000409 DAWAOG010000000
VERSION     DAWAOG010000409.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7545_length_1970_cov_2.279373"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          530 a          436 c          439 g          565 t
ORIGIN      
        1 tccttattgt ttcaagcatt ttactgcgta tcagcgttac cgagcccaca atgctgacaa
       61 ttgcggaaac agcgatcaga tatgcggcgg taaacaagcc gctttgtaca ttgattactt
      121 ctaccgaaaa gatagtgttt accgcaagca ggataagtgt agcggcaagc accgagatca
      181 ctgttacaga tgtatcacag ataagctcaa gtgcaagcat agagcgggtc ttaccgccac
      241 acagcctgaa taccgccatt gctttggtgc gcttttcggc attcagcagc acatttgcga
      301 ttactcccag agtgccgact atggctatca ttatgaatac aggcattaac tgtatcagga
      361 gcatattatc acggtacagc aagtcgagat ctgtcacatt atagcgtata ttcatgtaca
      421 gatcgccttt tgcgtcatcg ctgtttgcgt ccagtccgtc aattatacga gcctcgattt
      481 cggcgagaga agtattacat attgacggta gcggttatct ccgctttcgc cggaatgatc
      541 ttcctgattt tcgggtgctg ttgcaagccc ggagctatag agaatatctg catcggtgta
      601 gaaagttgta ccaaagcttg cggtgtccga attatacagt atcatactgt ccagatagtt
      661 gtcggtgtat ttgccgacta caagaagctc ggtgatccct tgcccgcacc tgtattttat
      721 tgtgtcaccg atattgcacc ttgccgaaca gccgacaatt acagggattt ttccgccata
      781 gtcctttgtc ttgtccggtc gttcacccga aagtcctata tcaaggtatt cactgtactc
      841 ggtcgaatat ccgagcagag atccgtttat atcattataa aaccgataga aagtgtcatc
      901 gtccatatta tttatctcgt tgtcggtaat atggctgttt acggagaaaa gctccaatgt
      961 agcttcatag taacgctcga cacggcaatt tattccgtac ttttcaaact gcgaagctat
     1021 cttttcatcg gttataggtg ctgttgacga gagtgtgccg tgcagtatct ttttatcata
     1081 gcggtggctt gtcagatagg acttttcggc aagatatccc gaagacgcgg tgaacgcgaa
     1141 attgaacagc agtgtcgaga atatgataca tatcatcatc aggataaaat tgcgcttttt
     1201 cgatttcagc agggagaacg caagcgataa cagcttcata ttgcacctcc gtttgtgtat
     1261 atgcctgagg atatgcgctt aaagcccggc agtaacgata ttatgactat aatgctgtaa
     1321 attacaaatg acagcagata gtcaaaagcg gtaggcacat attccattct gagctttacc
     1381 ataagtgtgg tgaaaagcgg gctcagggcg atcgcgcaga taaacgaaaa agccgtgtat
     1441 gtaagcgtga cagccgataa aagcagtatt atcggcatcg tcttacagcc tgtgaccttc
     1501 agcacgttta tctgttcgct gcaattatcc gtaaggtgaa gcataagcgt cagcaggagc
     1561 agagcggtga atatacccgt taccacgatc cctcctgtga tgatagcgta tatgatgata
     1621 acagattcgt tattctctct gttcggcggc tgaatataaa cctcctcgcc gagatattgc
     1681 ttaaacgctt tcaattggct gtcagacagg tctgatacaa agaaaaagcg tgtaccgctg
     1741 tattttgtag gggcggtatt cttcatctca ttttcaaaat cttcatcctt tacataaatt
     1801 cccgaattgt tgtgatatct gaaaaatgag tacggaataa taaaatcatc ggaaatacct
     1861 attacggtaa gcgtgatatc tcctttgtca tcaggaacga tcagtttgtc gccgacgctt
     1921 atgccacagt cactttctgc aataatgacc ttgctgtcac ctgtcagctc
//
LOCUS       DAWAOG010000410         1970 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7547_length_1970_cov_2.158225, whole genome shotgun sequence.
ACCESSION   DAWAOG010000410 DAWAOG010000000
VERSION     DAWAOG010000410.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7547_length_1970_cov_2.158225"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          593 a          384 c          554 g          439 t
ORIGIN      
        1 actggcaaag ggcgttgtca ttgaagcaag aatagacaag ggcagaggtc ctatcgctac
       61 tgttctcgta cagacaggta cgctccgcac aggtgacacg ataatcgcag gtactgctgt
      121 aggacgtgtc cgtgtaatgc gtgacgataa gggcagaaca gtaaaggaag caggtccttc
      181 tgttcccgtt gagatcatgg gtcttgcgga agttccgagc gcaggcgacg actttgcggc
      241 tgttgaagat gagaagcttg caagagagct tgttgaaaag agaaagttcg acgctaagga
      301 agaacagttc aagctctata agaaggtcag ccttgataat ctgttctcac agatcgaaga
      361 aggctcgatg aagaagctcc ccatcatagt aaaggcagac gtacagggtt ctgtagaagc
      421 tgtttcacag tcactgtcaa agctttcaaa cgaggaagta aaggtagaag taattcacgg
      481 tgcggtaggt gccgtaacag aaagcgatgt tatgcttgca aaggcatcgg acgctatcat
      541 agtaggcttt aacgtaagac ctaatcccgc cgcagctgaa aacgcaaagc gtgacggagt
      601 tgatataaga ctgtacagag taatctatga cgcaatagaa gaaatgcaga cagccatgaa
      661 gggtatgctt gctcccaagt acagagaggt cgatacaggt cgtgtcgaag taagacaggt
      721 aatgaagctg tcgagcgtcg gcgtagtagc cggttcttat gtccttgacg gtaaggtaca
      781 gagaaacggc gaggtaagag ttgtccgtga cggtataatc gttgctgttg ataagatagc
      841 aggcttacgc cgtttcaagg acgatgtgaa ggaagtagcc gcaggctatg agtgcggtat
      901 cacgcttgaa aagttcaccg acatcaagga aggcgatatc tttgaggcgt tcgttaccga
      961 ggagtacaga gactaatggc aaatttcaat acggcaagac ttgccgagga tataaagcgc
     1021 gagatttcca cagctatgcg tgatataaag gacagtgctg tagccaaggc aatggtcggc
     1081 gtatcacgct gtgagctgac aaacgatctt tcctactgca aggtctatat cacaacactt
     1141 gcgggaggcg acacaacaaa gactgttgcc gaacatctaa aaaaggctga gggttttttc
     1201 aaaagaagaa taaatgaacg tgtgaagatg cgcaagctcc ctcagctgat attcgtgccg
     1261 gacaattcaa tggattatta tgagcatatc agcgaggtca taagcaagct gccaaagccc
     1321 gccgaaaccg atgatgacgg cgacgacgaa aggtaaggtc aatgataaca gacataaaaa
     1381 acgcggcaaa gatactcaaa agcaatgatg actttctgat tttgtcgcac gcaaatcccg
     1441 acggcgatac gttgggttgc gcgtacggcc tttgcggagt gttgcagaaa atgggtaagc
     1501 gggcgaaggt catgtgtgct gatgaaatag caccgcgaat ggagtttctg cgtgaaactg
     1561 tgatacagca gaattttgaa gaaaaaaccg tagtgagcgt tgatgtagct gacagaaagc
     1621 tgctcggcga tcttgacgca gtgtacggtg ataagatata tcttgcgata gaccaccatg
     1681 aaagcagacg ggaatttgca aagtatacac ttgtcgatcc ggatgccgcg gcggcttgtg
     1741 aattgatcta tcagatagca gaagagatga atgcgccgct cgacggtgag atagcgtcat
     1801 gcctttatac cggacttgct acagatacgg gttgcttcaa gtattcaaat acgaccggca
     1861 ggactcatat aatagcggca aagcttatgg aacatgactt tgatgttgag ggcataaact
     1921 ataagctgtt tgatatgaag tcaaagggca ggttggagct tgagcaggtg
//
LOCUS       DAWAOG010000411         1969 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7552_length_1969_cov_2.347962, whole genome shotgun sequence.
ACCESSION   DAWAOG010000411 DAWAOG010000000
VERSION     DAWAOG010000411.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7552_length_1969_cov_2.347962"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          519 a          441 c          384 g          625 t
ORIGIN      
        1 atctttgacg gtgttacggt aagctccttt tttatgaaat tcatctttgc gctgtccgca
       61 tattcggatt tctcaacggc ttctcttact tcttcggcgc actgcgcaca gtcaagaccg
      121 tctattatat atttcttttc tttaaaagtc tgtctttcct ttactgttac ttcgggttcg
      181 tgctttgcga caacttcggt gaccgttgtc ataagctcat cgggcaaaca cgaattttcc
      241 gtttcaacgg taagctcttg cttcataaag ttcattcctg ccgatttcac acgctcgtcc
      301 ttttcgatct ccgcacggat ctcctccgca cagcgagcgc aatcaagacc ttctaaaata
      361 tagatttttt tcataagctg ccacctttga tatgcttgaa gttttgaaca attgagtaat
      421 tgttcaaatg tagagtgcga aaaaacagga gatgttatct cctttgattt ttcccgtttt
      481 attccataat atgttccagc gcattgctga acaccgtgtt tacatggtcg tcgctgagca
      541 tatagtaaac gaccttgcct tgcttttcac tgcgtacaag ctctgcctgt cttaatactc
      601 tcagttggtg cgaaattgcc gatgctgtca ttccgagcag ggcggcgagg tcacatacac
      661 acatctttga gcgtgacagc gaatagagga tcttgatcct ggtttcatcg cttatcgcct
      721 tgaaaaacat cgacagcttt tcaaactgcg tctgttcaag cattcccgaa cgtacataat
      781 ttactacgtc ctcatgtatc acgttacagt tacagaactc actgtcgtct ttttctgcaa
      841 agttttctga tttatcgact gccattttaa cctcatttca tttgaacggt tgttcaactg
      901 tttgcattat aacactgtca tactgcgttg tcaataggtt ctttatgatt ttttgctttt
      961 tctgcaaaat atttttcagc tatacaggaa aagaatgccg ttttatcatc ttccgtaaaa
     1021 tccttatcgt caaggagcgc actgacctcg gataacacct cgtctgcttt ctctctggtt
     1081 tctgccgggg aagtgccgta gaaatacagc ttttcatcgc ttgacagtat cagatccacc
     1141 gtgtcgtccg tcagcatttc ctcgctgact ccgagtagca ccgccatatc agaaagagtt
     1201 ttcgctcttg gcgttctcgc tccgcttatg taatttgcaa gcgctctgta ggttatggaa
     1261 atggctgccg caaaatcggt ctttttatag ccgtttcttt taataagtat ctccagcttg
     1321 tccttaagtt ccataattcc tccgactgaa tttcctttta ctattattat atccttacat
     1381 tggcgggttg tcaatcaaaa aaatacataa aaattcgcta aaattcaaca aatattcaca
     1441 atatgcagta taatatacag tttgctgtat aaaaactctt gactttgata cgaaaaggtg
     1501 tataatattc aatgtgagtt aaatcagaaa gcagaacgcc cgaaagcgtc tgtgagatag
     1561 aaaggaaata ataaaatggc taaagaaatc aaaaagattg tacttgctta ttcaggcgga
     1621 cttgatacgt ccatcatcat tccgtggtta cacgagaatt atcccggctg tgaagtaatc
     1681 tgcgtagcag gtaacgtagg acaggattct gaaatagtag gtcttgaaga aagagcaaag
     1741 aagacaggcg catcaaagct ctacatagaa gatatacagg acgaattcgt aaacgacttt
     1801 atattcccca cactcaaggc aggcgctaag tacgagggtt atctgctcgg tacatcattc
     1861 gctcgtcctc ctatagctaa aagacttgtt gagatagcta agaaggaagg cgctgacgct
     1921 atctgccacg gctgcacagg taagggtaac gatcaggttc gttttgcgc
//
LOCUS       DAWAOG010000412         1968 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7557_length_1968_cov_2.692107, whole genome shotgun sequence.
ACCESSION   DAWAOG010000412 DAWAOG010000000
VERSION     DAWAOG010000412.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7557_length_1968_cov_2.692107"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    470..479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          451 a          529 c          409 g          569 t
ORIGIN      
        1 gcttcatatt ctccttatcg ggcagaatac gtttaagcag tcttggatct gttttttcgc
       61 ctctcagtat cttctcatag aagttcctgg cggcgaagta tttatcctgc ggaaaatcgc
      121 ttctgcgtat atcctttact cttacactga cgctcttttt atcattatat tcgttaatct
      181 ccaaattact gagcacatct accttatctc ccggataata cccgaactta tcaaaaggca
      241 taccaaagca gagaaatttg aactgcgagc ccttgtactc tgctgtaaat gacgtgtatt
      301 tgccgtcttt aagcggtctt gacgataata ccgtacaatt tctcatcaaa aacagcggag
      361 cgttgttttc ttctccgtat ggctgtaaat gacgcagatt ttcaacagac gatatttcaa
      421 ggtcggaaag ctcaggctct atatccgctt ctactgtcat gacaggcatn nnnnnnnnna
      481 caaaatcttc gaccttatct ttcggcagag aaaagcctgc cgcctttgta tgtccgccga
      541 accgcgtcag atgttcactg cacgcgtcaa gtgccgtgta gagtgaaaaa ccctctatgc
      601 ttcttgctga gcctcttgcc tcatcaccct cgataccgat aactatacac ggcttttcgt
      661 acagctcaag caatcttgcg ctgactatgc cgattatacc gcggttccag ccttcgccgc
      721 atacaataag cactcgtgaa ttgaacgctt tagagtctga cataagctgt gcctttgccg
      781 aatcaagtat atcgctctca atttgctttc ttttcacgtt cagctcattc agctcctgcg
      841 cttttatctc ggctatctgc ccggtctgag ccagaaaaag ttcagccgcc gccttagggt
      901 cttcatatcg tcctgcggcg tttatgcgtg ggcagatacc gaacgcaacg cccgttgacg
      961 ttatattctc ctcgtcaagc cctgcttcgc gaataagtga cagcaagccc agattttcgc
     1021 tgtactgcat ttctgtaagc ccgcgcttta ctataacgcg gttctcgccg gtaagcggca
     1081 caacatcgcc tatagtgccg atagccgcaa ggtcggcata ctgctcaaga acaccttcaa
     1141 catcacgttc cagtgccata atgagcttta atgctacgcc acagcccgca agctccttat
     1201 acggcgatgt atcgtcacgc ctgtgcggat ttactatagc ttcggctctc ggcaggatct
     1261 cggggagctg atggtggtcg gttataacaa gcttcattcc aagctcatat ataagctccg
     1321 cttcattgac ggcggaaacg ccgttgtcga ccgtgattat aagccctgtg ccatcgtcgt
     1381 gcagtttgcg tatcgcgttt tcattcagtc cgtaaccctc ttcgcgggtt ggtatgtacc
     1441 attgtacatc tgcgccgata gcgtcaaggt gagtgtacaa cataacggta gatgtaacgc
     1501 cgtcacagtc atagtcgccg taaacagtta tctttacctc attgtcgacc gcttcctcga
     1561 ttgcctgtac tgccttatcc atatcagcta taagcagtgg gtcggaaagc tcagtaccgt
     1621 taaagtactg ctgtgcctga tcggctgtgt atatccctct tccgacaagc agactgcaaa
     1681 gcagtatcgg cagaccggta tcggtcttaa gcttcatcgc aagtgtattg tccgtatttc
     1741 tgacaagcca ttttttcata agaaatcata tcctcttgat aagttctctc aactcgtctg
     1801 atgtgaacct atatttatta tcgcagaaat ggcagcatac ctccgtgacc ggatcctcat
     1861 cagccagctt tgcaagctcc tccttgccga gcgttatcag cacctgctcg gtgcgttctc
     1921 ttgaacagtc gcagtaatac tgtgcttccc agctgtcaag tatctcgc
//
LOCUS       DAWAOG010000413         1963 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7585_length_1963_cov_1.982180, whole genome shotgun sequence.
ACCESSION   DAWAOG010000413 DAWAOG010000000
VERSION     DAWAOG010000413.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7585_length_1963_cov_1.982180"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1568..1577
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          620 a          371 c          488 g          474 t
ORIGIN      
        1 gtcggtatcc gatgacgaca gataccttga caagatcgcg gctcagctta tggaaaaggc
       61 acatactaag gacgataagt atgatgtcaa tgtgctttat accgccgagg aatgcatact
      121 gcggcgtatc gtcacaatgc ttttcagaaa taacgatata ccgctgaata tgagagcggt
      181 agaaagctgt gtcgggatca taagggcaag acagggaaaa ataaatccgt gccagtttaa
      241 atttgttatg atacgcaaaa aaaagctgtt tgtgctgact gactgtgaga aattcagacg
      301 aaattagtgt gataaacagc ttaaaagtga ttatcgccgt tgcttttcgg tagcattcgt
      361 gctataattg cagtattact gattttgttg caagcgctta acatttattt aatataagcg
      421 atgtattatt tacgataaag aaaaacggag aagtaaaacg tggaaaacaa catacacaat
      481 gatatcgaaa gagtgctttt cacaagcgaa cagcttatga aaagagcaga agagatagga
      541 aaacagataa ccgaggacta taagggcgaa gaggtaacga taataggtat actcaaaggc
      601 tcatttatgt tcctgtctga tgtgatgaaa agcatagacc tttacacaag acttgacttt
      661 gtgaccgtgc agtcatacgg aaacggtacg acatcgggtg agctcaggct tacaaaggat
      721 attaccgccg atatcaaggg taagcacgtc atactcgttg aggatattct tgacagcggt
      781 aagacgctta agtttgtaaa ggatctgttc cttgaaagag agcccgcatc aataaaggta
      841 tgcacattgc tcaataagaa agtaagaaaa tcagaagtta taaaggcaga ctatatcggc
      901 tttgacgtag ataatgtatt tgtagtcgga tacggacttg attatgccca gctgtacaga
      961 aatctcccgt acataggtga gcttaaggtc ggggcacgct gaaaaaacag cgtcgaagct
     1021 gtaaaaatat aaccgtaaag acggtcgggt gcaatatgca gacacgaccg acagaaaggg
     1081 aaactgatga atcagaacaa caaattcaaa ggcgttttca tttaccttgc agttatcgtt
     1141 ctgcttgtca taggcatggt cacaatgctt cagatgagcg cgacaccggg tgaacgaaca
     1201 agatattcac aggtgataag cgaatttgat aactacaatg tttcaggcta tacgcttgat
     1261 ctaggctcag gtgaattaca gtacacatta aagagcgaca gcacaaagaa atataagtat
     1321 tctgtgccga acgtaagcct gttcttacag gataccgagg gttacagaaa ggcatataac
     1381 gagaaaaatc ccgacagtcc gctcgttgag gacttctatc ccgtttcgga caattcgttc
     1441 ctgctcagtt tcctgccgta tctgcttatg gtagcactga tgataggctt tacgtttgtg
     1501 ataatgcgtc aggcaggcgg cggcggaaaa atgtcgcagt tctccaaggc gaacgcaaga
     1561 acacagcnnn nnnnnnngtc ctaagataac ctttgccgat gttgcaggtg cagaggaaga
     1621 aaaggaagaa atgagcgaga tcgtcgactt tatgagaaac cccagaaagt atcaggagct
     1681 cggcgctaaa ataccgcgcg gcgtactttt gctcggccct cccggtacag gtaagacctt
     1741 acttgcaaag gctgttgcgg gagaagcaaa cgttccgttc ttctcaataa gcggttcgga
     1801 tttcgttgag atgttcgtcg gtgtcggcgc aagccgtgta agagatctgt tcgatcaggc
     1861 aaagaagcat acaccgtcaa taatattcat agatgagata gatgcggtag gtcgtcagag
     1921 aggtacaggt cttggcggcg gtcacgatga gcgtgaacag acg
//
LOCUS       DAWAOG010000414         1958 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7613_length_1958_cov_1.911193, whole genome shotgun sequence.
ACCESSION   DAWAOG010000414 DAWAOG010000000
VERSION     DAWAOG010000414.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7613_length_1958_cov_1.911193"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1455..1464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          515 a          459 c          480 g          494 t
ORIGIN      
        1 ttttaagaaa cgaccttgag caaagcggtt atgagataat cgaagagggt ctttgcggaa
       61 gaacaacgat atttgaagac cccgacagga taggcagagc gggcgataag cttctgccgg
      121 tattcttgga aagtcacgct ccgcttgata tggtcgtgat aatgcttggc actaacgact
      181 gcaagcccgt ttataacgct gacgcagaaa cgattgcgca cggtgcggag cgtcttatcg
      241 agcaggtcag aatgtacgcg ggcaactgcc gtatcctgct catttcacct atagagcttg
      301 gaaatgacgt atggcaggat aaatacgacc cgcagtttga cgcaggctcg cccgccgttt
      361 cccgcggtct taaatcttcg tttgcaaagg tcgcaagaaa gcacggtaca gatatccttg
      421 ccgcttccga ccacgcaaaa cccagcagca tagactgcga acaccttgac gaaaacggac
      481 acaatgcgct tgcccgtgca gtcgaggaca agatactcag tatttacgga agggcggctt
      541 cataaaccaa atgacaaaaa agcaacttat caggcactct ttcatcaaga gtctgcctgt
      601 actgtgcagt tatctgttct taggtatggc atacggcatg atgatggcag aggcgggata
      661 tcagtggtac tactcgctgt tctcctgtct gttcctctac tccggaacct gccagttctt
      721 acttataaca tttttcagca gcggagcgtc actacttacg gtggcgctca ctgttctgct
      781 tgtaaacagc agacagagct tctacagcct tacatttctg aaggatttca gcgctatggg
      841 aaagcgtaag ctgtatatga tacattcgat gaccgatgaa acctatgcgg taaacactac
      901 actcgactat atgccggagg acgacaagca cagcaccatg ttcggcgttg cgcttttcag
      961 tcatttctac tggactttct tctcggtgct cggaagcgtt atcgggcagc tgctcccgtt
     1021 tcagcttgag ggcgttgact tctgtatgac agcactgttc gtcattatct ttatcgacaa
     1081 gtgggaacac gccgaccggc actcccctgc gcttatcggt cttggggtag gcgtactgtg
     1141 cctgctgatc ttcggacaga acagctttat gctccctgcc ctgctattat ccaccgcgct
     1201 tttactgctg ataaccaata cggatttcgg caagaagaaa ggcaagaagg ataaagacgc
     1261 agacggcgaa aacaccgaaa acaaaaccga tagcaagaca atagaagtcg ttacttctcc
     1321 ggaagatatt atcaccgatg aaaaggagga taagacgata tgacatcgac tcctatgact
     1381 cctttacaga tggtgatagc gatagttatc agtgctgtta tcacattttc tctgcgtgca
     1441 ttgccttttg tgatnnnnnn nnnncccggc tatgataatg tcgatactta taatctactg
     1501 cctcaaggat atcggcgaca atatgttcgg tgtcggtata ccaagacttc tcgcggtact
     1561 tatcacaggc ggaagctata agctgtggca taacactttt gtaagtatgg cggcgggtac
     1621 gctgtcatat atgggtctgc tgtacttatt cagccatgtg ctgacaggtg cataagaaag
     1681 gaaataaact ttggaaataa ccgtaagcga gcttgaaaag ctctcccccg acagctatat
     1741 tttatacgat acccgcagtg aaaccgaaaa aatgtacggt attatggagg gtgctgtgtc
     1801 gctttcggca gatgagcttt tatctgcacc gcccgaggac aagacgaaaa aacttatcgt
     1861 atgctgtgca agaggaaaat tcagcgttga tactgccgaa agattatccg agatgggcta
     1921 tgatgcctgt tcgctttcgg gcggatatgc cgcatggc
//
LOCUS       DAWAOG010000415         1958 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7610_length_1958_cov_2.429848, whole genome shotgun sequence.
ACCESSION   DAWAOG010000415 DAWAOG010000000
VERSION     DAWAOG010000415.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7610_length_1958_cov_2.429848"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          645 a          457 c          450 g          406 t
ORIGIN      
        1 ctgccgcagt tgaccagaaa tcgtggaagc ttatagcaag aaccatgtac acccccgaaa
       61 aaggcaaatt cggctacccg ttcttcagtg acgctaccga tgagagcggc gcgatactta
      121 caaagataga ccgtatagta ctgcgcaacg gcgcaaacga ccgtgaattt gcaggcgttc
      181 gcgatgagct ttcaatgtcg cttgcaaagc agtcgggcta tcttgatgcg caatcgaccg
      241 cacctgcggc agtcttcctt aacggaaaat attacggctt tgcatggctt caccagaatt
      301 tcagccgtgc ataccttgaa gaacgctacg gcggaacaaa agacaactat caggtagtcg
      361 gcaaggcaga gggcgagctc gataacgaga acgcagaggg cgcgatagct gactataata
      421 agatgcttga gcttgccgaa agcggtctta ccgatgacaa gaagtttgaa cagctctgct
      481 ctatgatgga tattgacaac tatatgcact atcttgcaat gcagctgttc attgacaacc
      541 gcgactggcc cggcaacaac tacaaggtat ggcgctatgt agcgtcagag ggtgaagagg
      601 tcacaagcaa atatcaggac ggcaagtgga gatatttctt ctatgacgcc gagtttgcat
      661 ggggacttta ttcagacgga tacgcgaaca aaacgcttac aaagatactt aaaggaactc
      721 accccgcagg cggctctgca cttgtatcgg cacttatgga acgtgccgat atgcgtgaaa
      781 agcttgcgaa caatatctgc gaccttatcg gcggcgcatt cagcaccgaa aatatccttg
      841 ccaccctcga gcaaaagctt gccgacagcg ataaggagca gctgtacgcg ctgaacaagg
      901 gtataacctc agagtgggca aacgagggta cgtttgaaaa cagcagaaac gaaataagag
      961 aatttgccga caagcgcgca aacataatcc tcggcgatat ctgccgcaac tttgagatag
     1021 acaaggacga tacatacaag gtaaagctga acggttcaaa gggtcttaag gtcaccatga
     1081 acattcagac cgtcaaggat accaatacga taagcgcaga gtatttcaca ccttataagg
     1141 taaagcttac cgcagaggat ttgtccggct acatcttcac aagctgggaa ataaacggca
     1201 cgacatacac cgaccgcgaa ataacgattg acagctcaat ggctaaaaag ggaaagataa
     1261 ctataaacgc ccgcagcgaa aaaaccactt caacagatga actgctgcac ataagcgagg
     1321 tctacacagg cggtgacgca gactggatag agctttacaa tcccaatgac actgacgtat
     1381 ctacaagagg tctttatctg accgataagg aggatatgct caaccgatat aagataccga
     1441 cagtcaatgt aaagccgcac tcaacgctta ctatagtctg caaaaacaac aagtctgaaa
     1501 atacgctgat gaaaatgcag acaaatttca gccttaaaac aggcgaaacg ctgatactaa
     1561 gcaacgaaaa cggcgatatt ctcggcaagg tcgcaataat tgactgtgac gagaacgaat
     1621 cgctcgtccg tcagcgtgac ggaagctacg caaagggtac gccgaccttt gaaaagaaca
     1681 atacataacc aaactgtaaa aaacagccgt acatccgggt ttatccagaa tgtacggctg
     1741 gtttttaaga caagggactg tgacacaatt cctaagctta tcaaattaca ttaaaattac
     1801 gatgcaatat attgtaaaag catcaaaaca ataaatacac tgccaacaac gctgaaaaca
     1861 gcttccatgc ttgcaaggtg ttaataattt cgttagtaaa tctgtgcaac tgcactgtca
     1921 ccgactatcc aaaaagaacg ctactatcct gagatatc
//
LOCUS       DAWAOG010000416         1954 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7640_length_1954_cov_2.153765, whole genome shotgun sequence.
ACCESSION   DAWAOG010000416 DAWAOG010000000
VERSION     DAWAOG010000416.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7640_length_1954_cov_2.153765"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1419..1428
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          481 a          458 c          466 g          539 t
ORIGIN      
        1 gttttataag taattgacag ttattccata tttgttataa taccgtcact tggtagcgca
       61 atggtaatgt caatttacac acttacagat gcaattgtaa ttggacaggg agttggggct
      121 aacgcccttg tggcacttgc cggagtagtt agatgtgcaa aggattataa aatcccatga
      181 ggcaaactgt actccctgat ctgagtgcag aatcacttta gggtagtttt ctttttccca
      241 gtcttgtatc ccagtttaga atgtatgatt acggcatata aatttaattc tttattcatg
      301 tatttgtgca tagtggtatt gcttatttaa gcaatattat aatatattat tcgaacaagg
      361 tgttacaagt ttagtttacc actacacctt caggaataca aaacctgaag tctcgtaaga
      421 cgcagttgag gaatagacgg gtttatacta taatttaatt tgaaaagtta tacccaaggg
      481 taagcaaaaa cgaatgaagg cgaaatgggg tatgtttatg accgaacaga aaaagaatac
      541 tatcataaca agcggtatcg ctgtgcttgc cgtgatactg gcatattgct tccgaattgt
      601 aggcaggggc agtttctacc cgatgctgtt ttcctatctg cgcagcttta tctatatcgg
      661 tctgttcgct gcctgggggc tctccgtccg tcagcggatc gcgcaaaagc aggtgcgcag
      721 gtttatgacg gacaccgcgg tgcttctcat catctggatg gtagtccgtt cggcaaagta
      781 ttttatattt tggcagccgg atgccgtccg gtacctgtgg tatctgttct atctgcccat
      841 gctgtttgtg cctatgctgg cgctgctgat tgccatgtca ttgggaaagc cggacgaata
      901 caaattcccc aagggaatgt cggttctgtg gattatctcc ggcgtattgc ttctgctcgt
      961 gctcacaaat gacctgcacc aattcgtatt tacctttccg aaggacgccg ccgtgtggac
     1021 ggacaaaaac aacggctact cggcaggcta cttcattagt gtgggctggc aggtactatg
     1081 tgcctttgcc gcactcgtaa ttatgttttt gaaatgccgc gtgcaaaaag gaaggctcca
     1141 ctttctgccc attgttccta tgctgctggc gattgtttac agcgcacttt actatgcggg
     1201 tgcggattgg ctgctccact tgttcggcga tattgccgca ttccaaagtg tgatgtacat
     1261 tctgaccttt gagttttgca tcgcctgcgg atatatacat tcaaacagcc gttatgtgga
     1321 cttgtttgcc tcctctgtcg gcacatcggc agagattacc gacaaggact ttaccgttcg
     1381 gtatgcagct gtaaacaatg cgcctatccc aaaggcaann nnnnnnnnca caccatgccc
     1441 attgacggcg gctatgccgt atggacagag gatgtgtcgg ctctgcgcga tatcaaggag
     1501 gactccgagc ttctcgcaga cgagctcgcc gaccgaaacg aaatactccg ctatgaatac
     1561 aagcgtgaag ccaagcgccg caaggtggag gagcaaaacc gcctgtatga tctcctgcgt
     1621 tccactacgc agacgcagat cgaccgaatt gcggagctca ctaaggaata ccgccgaatc
     1681 tcaagcaccg atcccgacag cgcaaaaacg ctccttgccg agattaccgt gctgtgcagc
     1741 tatattaaac gccgcaagca tctgacgctc cttaccgacc gagatattaa aatagcggca
     1801 acggaactcc accgtgcctt caatgagtcc ctccaaacgc tgaagctgtt gggcgtccgc
     1861 agctcgctct atgtggacga gtctctttcc atgctctccg gcaaaacggc gaccgctgta
     1921 tttgattttt atgaatcggt tattgaagcc gata
//
LOCUS       DAWAOG010000417         1954 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7638_length_1954_cov_2.469194, whole genome shotgun sequence.
ACCESSION   DAWAOG010000417 DAWAOG010000000
VERSION     DAWAOG010000417.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7638_length_1954_cov_2.469194"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          548 a          517 c          439 g          450 t
ORIGIN      
        1 tcagcggtgt tggctgtatg tttcataata ctgctttatc ggaaatgtgc tgtaatgaga
       61 attattgcct atatacaaaa aggagttgtg acgcaaactg aaaaagaaaa agcgagccga
      121 gcgcactatt ctttgcgctt cggctcactt ttcctttttc ggggttttgt cacagctccg
      181 ttgatatcaa atcaatgtaa aatcaaaatg cgccgctcac cgtaaaagct ttgcctttgc
      241 ggatataacg gttgtagaaa aacagatcca cgatcagcgc aagcacccag cccacaggcc
      301 acgacagcca tatgccgtcc gagccgaaga acggagccag aatgtacgca aacactacac
      361 gcaatgcaag atcaaggcaa gtacttatca taaacggtgt cataacgcca cagcctctca
      421 caccgccgtc aattactatc tttatgcata caaagaagta gaacggtgaa acgatagtca
      481 ggaacttacc gccggttgct attatctcgg cgttagcctg cgttgcgaac aggtttacaa
      541 gcgttcctcc gaacaaaata taacaaaccg caaacggcac tgctatcaca agacacatca
      601 gcatacccga ctttacgccg gttttcacac ggtcggtctt gttagcgcct atgttctgtg
      661 cacaaaaatt cgacacgctg tttgaaagcg ttgaaaaaca ggttatggca aaagtattaa
      721 gcttgatagc tgacgcatag ccgccgatga ccgatgagcc gaagctgttg acaaggctct
      781 gcacaaacag gtttcccacc gatacaaagc tgctttgcaa tattgacggt atcgaaatac
      841 gacttatctg tttaagcgct tccgaagaaa atattttcgg cttttcggga gtatccgact
      901 gtattgtttc cacacgtctt aaaagcacaa agaaagccgc cgcacctgcc gcgccctgag
      961 cggcaaacgt agcccacgca acacccgcaa cgcccatctg aaatactatt acaaagagca
     1021 gatccagccc gatatttccg agcgatgacg ctatcaggaa aaacaacggt gttttagagt
     1081 cgccgagagc cgtgaatatg ccgttgcacg cattgtagat aaacaggaat aaaagcccca
     1141 gaacatacac cctgagatat atgtcgctgt ccgcaaatat atcctccggc gttccgagaa
     1201 gtgtcagcat aggtgagcac aggacacagc ctataacggt aagtgccgcc gctataaccg
     1261 cgaacgaaac cagcgccgtg tttatcgccg tcttcatata tgtatagtct tttgagccga
     1321 acagccgcgc aacaacgacc gacgctccca cgcttccgcc tgtcgccacc gccataaata
     1381 tcattgtcac ggggtagctt gcgcctatcg cgccgagcgc catatcgctt acaaagttgc
     1441 cggctattac gctgtcaacg atgttgtaca gctgctgaaa tgcaacactg agcagcatcg
     1501 ggagggaaaa tttcagcaga accgaaaagg tcctgccgtt agtcatatct gtgaccattc
     1561 ttgaaaatcg cttccttatt cataaagcct ttcggagcgg cttttgtcag ccttgcggca
     1621 tagtccttta ccatatccgc aaactgtaat ttttttacaa agcccgaggg gatctcggaa
     1681 tatccgagca gtgcgccttt cagagcaccc gcgataaacg cgcatatatc gctgataccg
     1741 tcctggtttg ccgcaagctg taccgcgctg agatagtcat aatgcacaca agcgcagtac
     1801 acgccgatag caagggcgga gtccgccgta ctgccgtttc cgagctcggc aacatcggta
     1861 agcggagatg tgttctcatc aagtaatgca agcgccttgt caagcgccgc aaagcaatcc
     1921 tcaaagccgt catagccttt gagtatcttc atcg
//
LOCUS       DAWAOG010000418         1944 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7678_length_1944_cov_1.917417, whole genome shotgun sequence.
ACCESSION   DAWAOG010000418 DAWAOG010000000
VERSION     DAWAOG010000418.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7678_length_1944_cov_1.917417"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1175..1184
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          622 a          355 c          429 g          528 t
ORIGIN      
        1 catcacttgg tgcgtgcact gccaccgaca cggacaaaga taacgataaa cgtaattttg
       61 ctgagatgaa gtctgacagc gagcttgctt ataacacaaa gggtgagttc tcggttaatt
      121 taaccacgga taatattacg cttaacaacg acataagtgc agacagtgta aaagtattct
      181 attcatatat cgacagcgac aagcttccag atattgatgc atcgcaggcg gtaaagctga
      241 acagtgacga gtattcggca atgtacgcca aggttacggg gttgaccgtt aactcggatc
      301 actcggcaac agtggcattc agcgacagta agttcagtga aaacaagccc gattgtttct
      361 ttgtgctgtt tgataagaag accaatgctt cgggaaggta tcttgtttcg atgatacctg
      421 taaagtatac ggcgtattca cttgtcagtg atacaacgca gatacgctcg gaaagcacta
      481 gcaacaagtt taagcttaca cttgataaaa gtagctttgc cgccgaggtt tcggcagatg
      541 atattgtatt atctggcggc tttgaaaact gtagcatcgg caatattgaa aaagtaggag
      601 ataatacatt atctttggta ataaatgccg aaagtggtta taaaacaggc gatgacggtt
      661 gtattacggt aaaacacaga gctgttacgg atgcggcagt tgatgtttcc gcaactgttg
      721 atattatatc tccgtctgct gtatttcaat caaacagctt tgaagcttcg accaattatg
      781 caaggatacc tgtatcattg actgactgta cattcagcga cagcgttacc gtcggaatgt
      841 tcaattgtga taacaataat attgaaatta cacggtttga cagagtatca gcaaatgaag
      901 gcatattgta tctttcattt gataccgatg cgccgtcaaa agctatttct ctgatatccg
      961 acagtacatt cagcatagaa gataaagcgc ttagtattaa ctatccgctg tactttaatg
     1021 taacaccaaa atcacccgat ttgagtgctg taattacaaa agtcgaagaa gatgcatccg
     1081 actttaaggt aacagctgaa tttagtgtgg tagacggtac tttcaatgtg ataagcaaaa
     1141 acagctttat ctttagcggc gactattcca aagcnnnnnn nnnngcgcag taatgagcag
     1201 atggggtgta cagacaaata tcgcggcatt tccgctgtat tacagtgcta cagagcaaaa
     1261 cggtgatact gccgataatg cagacaatga tgagttacag gatatgctga aagctataaa
     1321 tgaatttttc ggttctatca gtatagccga tgagctatca ttagacggat tatctgaagc
     1381 aacagcagga aatgctaaat tcggtgagct ttacgaaaaa ctaagcgata agtacagtta
     1441 catttacaga tcagtgaaaa gaacaagcaa actgcttaat tcagacagaa tagcatcagg
     1501 tacggataat tcagaagctg tgtacagtgt cgagcgatat atgaacgatc ttttagcgat
     1561 gcatacgctt atacgacctg tcagtgacag catatcaagg ttatttacgg tatatgaaca
     1621 aatgagtaat acgaccgaca gtcaggaatt agacgctcta agcagtgaag ctaaagaact
     1681 gaccgccaaa gtaacaactg tatatgcatc taaggttaag ggtgtatctt acagcaaact
     1741 gcttgaaaga attattgacg gatattcagt gggcgcactt gcttcttttg atgccttgtg
     1801 cgatgaaatc tataattggt atccgcagag catagatgat aaaaaaatgt tcagaagtat
     1861 gtcgcttagc gttataatca atgccacagt tatcgaatat atttctgctt caggttcaga
     1921 agccaattcc gatgagttca ggaa
//
LOCUS       DAWAOG010000419         1943 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7683_length_1943_cov_2.341631, whole genome shotgun sequence.
ACCESSION   DAWAOG010000419 DAWAOG010000000
VERSION     DAWAOG010000419.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7683_length_1943_cov_2.341631"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    685..694
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    927..936
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          540 a          461 c          318 g          604 t
ORIGIN      
        1 agcttatgcg gagcgcgtca gccgctctgc cgccgagctt aacgcctgtt accttagaaa
       61 gcgtaggtaa cttgtcaaga agtatgtaat caaagctgtt ttcttttgca taccgctcac
      121 cggctgtacc tctatagcaa tagattttga agttggatat cttctttttt tcattatcat
      181 aataatagcc aaaagcatct ttaccgatag ttttaacact cacaggaatt gtaactgttt
      241 ttagtgattt acagttgtag aagacagctt catctatact ggtcacacta cccggtattg
      301 ctatacttgt aaggcttgtg caaccgttga aagcatccca acctatcttc gacacactac
      361 cgggtattgt tatacttaca agactttcac aattcatgaa agcataacta cctatactta
      421 tcgcaccatc aggtattgtt atactcataa gatttttaca attttggaaa gcagaatttc
      481 ctacagctat ctccgtcacg ctgtccggta ttattattct agtaaggttt acgcaacttc
      541 tgaaagcact atagcccata cttataacac tatccggaat gatataactc ttatctgttt
      601 ttccggcagg atagcatatt aactctgttt tatctttgtt gaacaagacg ccgttcacag
      661 acgtataatt cttgttacca atggnnnnnn nnnngtgcaa tctccaaacg cactgtcgcc
      721 tatactcgtt acgctgtccg gtatcgttac gcttttaaga cttgaacaat cactaaacgc
      781 aataaaacct atactcatca cgctgcccgg tatcgttatg cttgtaaggt ttgcacagcc
      841 ccagaaagct ctatcgccta cactcgtaac gctatttggt atcgttacgc ttgtaaggat
      901 taaacaatct ttgaaaacgc tatcgcnnnn nnnnnnttct tgccgtttat ttccgacgga
      961 acggtgaaaa ttttgtcaac acccgcatat cccttaatag ctagcgttcc gttatcaagt
     1021 tcttccgtaa ccaaacaagt ttcgcttgaa aaaccgtatt tggttgcata ataatgacca
     1081 tatgtgttct tcgtatagtt tatgctgaaa ccgtccacta tcgtatgctc atcatcataa
     1141 atcccatatc caaaagcttc gggaccgata tatttaacac tggctggaat tgtaactgat
     1201 ttaagagatg tgcaatgata gaatgctctt tcatatatct ctgttacatt atctggtatc
     1261 gttacgcttc ttaggtttgt acaaaagtag aaagcataac ttccaatact tgtcacacta
     1321 tcgggtatcg ttatacttgc tagacggcta tatttgaaag catcatagcc tatactcgtc
     1381 acactcttcc catcaatttc agcaggaata tcaacctttt ccgcactacc gttataatcg
     1441 gtaatctcca ctgtaccgtc atcaagcaca ccgtactcaa aatccccata agtctccgcg
     1501 cctgcgcaca ccacgcaaag cacagccatc gccgcaaagg tcataaaccc caacagccac
     1561 tttttcactt tcataaatac atacctcttt ccttttaaaa tacaaaaagg cacagtccgc
     1621 cgtgagctgt gccttgatat cgatatgtat accgcctctg atatcaatat atctcccacg
     1681 gataatagct gtatccctat gctttaccgt gttttcagta tatcaccgcc ggtagttatt
     1741 gtcaagctta tttctctgct ttttccatat agcaccgaca atacacccac agataaatca
     1801 aaggcaccga ttgctcgatg cctttgttct gttattcagt tataaaaccg ttttcaactc
     1861 agttctgctg cattaaagct ctgtagggtt caaggtaaga ttctacagtt ccgaagtaag
     1921 aatcacggag acttgtatag gat
//
LOCUS       DAWAOG010000420         1943 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7686_length_1943_cov_1.829449, whole genome shotgun sequence.
ACCESSION   DAWAOG010000420 DAWAOG010000000
VERSION     DAWAOG010000420.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7686_length_1943_cov_1.829449"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1567..1576
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          461 a          478 c          380 g          614 t
ORIGIN      
        1 atattgtcgt aagcgctcgc ccttacggta gcgtcgttat cattgctgag cattttcagt
       61 atttcaacag cctgctttgc cgtgtgatcg ataagtatat ctgccgcatc acatttaaga
      121 cacatatcat tgctttttgt gatatcgttc agaaattcat aatcgtcatc gctgagatca
      181 tctttctctt gcatttccga aagccgtttt tcggcttctt tatagttcat tcttacctcc
      241 ataagcttaa gtatcataat atttaatgca tctgtgacaa gcaacgtcag cccttattct
      301 tcctgtactc atcataagaa ataccgaatt ttctgaaaat gtactccgca tttcctgcct
      361 tatccggcat acgtcttgat atcacatagg ttggcttgcc gttttcgtca cattctgcgg
      421 taaggcttgc aagtgtgctt ttcggctttt cttcattgat tttctcaatg ctgtcggtaa
      481 gctccttgat atgagttgta tagataagcc ttacgccgat atcggcaaat atcctcatga
      541 tctctcttgc aataataaga cactcggtag gcattgtgct tgaaaggctc tcgttcatca
      601 gcaccaggct gtgatcggta gccgcgccta tggtatcgcg gaactgcttg cattcctcgg
      661 tgaaacggct tgcgtttata cctatttcct cttccttcgg aaaatgaaca aatatattgt
      721 cgacaggcga tatctcggca ctttccgccg aaacataaag tccgcactga gcgaacacct
      781 gcgctattcc taccgctctt gtaaaggtcg ttttaccgcc gttattcggt cctgtcagta
      841 taagaatacg accgccatcg tccatacagc agtcattctg cttcacggtg ctatcattca
      901 cgccgacata gcttgtacac atcttattgg caaatgcagt gtcatacagc tttttatagt
      961 ccgctctgcg ctcgttcatc ggcagtatga caggtctgca cattggaagt cccatacttc
     1021 tcatacggct gaccgtattc gccgctccta tgtagaaatt tatctgcttt tcaagtgtga
     1081 taagaaaatc tatgcttgcg tcataataag cccttagcgc cgtgttgaaa tgctttgaat
     1141 attctccgcc cagtgcgtca agctccttga acagcctttc gttgatcgat gagatcgcgc
     1201 cgtccttgct ttttcttgta tagacatttg taagcggttc taccgagctc tgaacgccga
     1261 gaagcttatc aaacagcttc ctttccttga acggttcgcg gcttacactt atcagcgcta
     1321 tttcctcagg gcgcataagc tcgtcaagat ttaccccgaa tgtcacactg cgaaaacctg
     1381 ttgccagtgt ttttctgagc tcggcgatct caaccttcag tgtagcaaac tcttccgatc
     1441 tgtatttatc ctcaatagcc tgcactacgg cacgcatacc tgcggaaaca agtttcccac
     1501 agcatttttc ataaaatccg tggcactcct ccatgcagga aatgaacgac tctataagcg
     1561 ccgtatnnnn nnnnnntcaa agaacgactg agtagagccc gcctttgcat agaccgattt
     1621 tgagtttgca taaatagtat gcaggctttt ataaagaatt gcctcaagct caggtacatt
     1681 tataaaatca tcgactatat cctatcttta gcttatgacc tcgggatctg tcacaagctg
     1741 tgacaatatc cgctgtgcat actctgtatc atacggcgaa agcgtcattg caagatattc
     1801 tatctgcaga tcagccgccg cgcttttagc aagctgattg tatctttttt gctttgcgtc
     1861 tgccgggaat aaaagatcgg gtgttgccat aatgttatcc tcttttcgtg aagtgttgcc
     1921 tgataatttg atcatatatg ctg
//
LOCUS       DAWAOG010000421         1931 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7768_length_1931_cov_2.078891, whole genome shotgun sequence.
ACCESSION   DAWAOG010000421 DAWAOG010000000
VERSION     DAWAOG010000421.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7768_length_1931_cov_2.078891"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    441..450
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          608 a          424 c          494 g          395 t
ORIGIN      
        1 tctgtatgct tctcagaaag cacctcagcg gaggaaaact gctcgatata acgcaggacg
       61 gacttgagcg tataataaac tttgattttg agtgcatgaa cgaaataggc gatatggtag
      121 taaaccgcct taccgccgaa ataatgggca ggcacagcaa tataatactt atggcaaaaa
      181 aggacggcgt gtaccgtgtt accgacagca taaagcgtat cacggacgat gtttcttcgg
      241 taagacgcat actgccgaat atcgtatacg aaacgccgcc gagagatctg acccgtgtaa
      301 acttccttac ctgttccgac agcgagttat aaacgctgta aaacagggag ggggaaaacg
      361 gctgtcaaag cacctgaccg cctgccttga gggcgtttct ccggtatttg cgagagagtg
      421 cgcttattat tcgtgcaggg nnnnnnnnnn cgatagctat gaccgtttgc tgtttttcat
      481 aaaacgcgca agggaatatg tgacaaataa aaacggattt acgattctgc gtgaccttaa
      541 cggcgcatac aaggatttct gctttatgcc gatagcgcaa tacggcacgc agatgcttgt
      601 atcccacgca gacggcgcaa atgcactgct tgatatattc tacggcagta aagcggaaaa
      661 cgaacgtatg aaacagcgtt caagggatct gctgaaaatg ctgatgaata cctatgagcg
      721 aatagcaaga aagctggagc ttcagaaggg agagcttgca cagtgcggag aaagggaggt
      781 attccgagtc cgtggcgatg ttgtaagtgc gaacatatac cgtatggaaa aaggcatgac
      841 cgagcttacc gccgaggatt acagcacggg cgaaaccgtg accgttccgc ttgacgtaag
      901 gctcaccccc tcacagaacg cccagaaata ttatgcggaa tacaagaagc ttgaaaaagc
      961 cgaaagaatg cttacaaagc tgatagcaga cggcgaaaac gagcttatat atatcgacag
     1021 cgtattcgat gccgcgtccc gtgccacctc caatgcggag ataagcgaga taaagcagga
     1081 gttagcggca aacggctata tccatgcgaa taccgtcaat aaaaaaacac aggtaaaacc
     1141 gaaacctcct atgcactttg tgaccgatga cggttacgat gtgtatatag gcaggaacaa
     1201 catacagaat gacaagctga cagtaaaaac cgccgagccg gacgatatgt ggcttcatac
     1261 gaagaacata gcaggctcgc acgtcataat aaaggcgaag aacggcgaaa tatcggataa
     1321 tgccattctg caggcggcaa gccttgcggc atactgctca aaggcacaga acagcacgaa
     1381 agtgccggtt gactacacac ggataaggtt tgtaaagaag cccaacggcg caaagccggg
     1441 tatggtgata ttcacaaaca attacacggt gcttgtagac cccgatgaac agcttttcgc
     1501 aagggtggaa gcgtaacgtt aaactccggc aatcaaagaa aggatataaa taaaatgaag
     1561 aaagaactcg caaagaccta ttctcctaag gattttgagg acaggcttta tcacgaatgg
     1621 gaggaaaaga agtacttcca tgcagaaata gaccccaaga agaagcccta tacgatagtt
     1681 atcccccctc cgaacataac cggacagctc catatgggac acgctcttga caacacattg
     1741 caggatattc ttatccgcta taagagaatg cagggctact gtgcactgtg gcttcccggt
     1801 acagaccacg cttctatcgc tacagaggcg aagatcgtta atgcgatgaa ggaagagggt
     1861 cttaccaagg acgatgtagg aagagatggc tttcttgaac gtgcgtggga atggaagaga
     1921 gtttacggcg g
//
LOCUS       DAWAOG010000422         1926 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7806_length_1926_cov_2.355425, whole genome shotgun sequence.
ACCESSION   DAWAOG010000422 DAWAOG010000000
VERSION     DAWAOG010000422.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7806_length_1926_cov_2.355425"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1222..1231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          518 a          414 c          388 g          596 t
ORIGIN      
        1 tcgctgagtg ttacagcctt gatgtatttg tcgtcaaggt cggctatagt gatatccgag
       61 aaatctgtat caggaaggac aagattattc ttgaccttga gaaatcgcat gaacatacgc
      121 aggtccgtat aatagttcag agccgtaagc tgtgaacggt ttttgatggt agtcagataa
      181 ttcaggtatt cggtaagaca agccggtgca tctgatgtag cttgtctaaa atctatgttg
      241 tatgccattt ttgttgcctt tcgggttttt ggtacttcta atttcgtaaa attttcattt
      301 tacgaaatta cgtgtaaaat tctgttatac ttctccgttc accattatac aataaatctg
      361 ctgtatttac aagcaaaacg gtaaaataaa atcagtgcac aacaaggata caaacggttt
      421 tgcgaccttc aataatttgg tgataccgaa attatatttg tatgatattt ttgtatctgt
      481 gcactgattt aaatttgtta aaacttatac ttcgttcttt ataatgtcga tcacaccgtc
      541 aagagcttca cattttgcgt aagccttgtc ggttatttcc gacggtacat caacaatgtc
      601 ttttatatag acagtcttta tccctgagcc tatgcctgcc ttaagaccgt ttgtgctgtc
      661 ttcgataaca agacaatttt ccttgctgta tcctgacaac tcagctgctt taatgaatat
      721 ttcagggtcg ggcttgcctt ttgtaatctc atcgccgccg attattccgt caaaatatcc
      781 gcggatatcc gagtgttcaa gttcttttgc cgctgaagtg tgatatgtag acgttgcaac
      841 gtacattgcg atgttgtttg ctttaaggta tgcgcaaagc tcgctaaatc cttgctttac
      901 cggaatgcca ttcttctctt cgtattcggc aaaatattgc tttgcatgat tccagtattt
      961 atcaaacgga taatcctcgc cgagctctcc gagaaaaaga cttttcattg aagtataatt
     1021 aagccctatc gaacgatacg gaagtgacaa atcaaaaggc agactgtatt tgtcgataac
     1081 atactgccac gccttaagcc ctatcctttc actgtcaagg agcgtaccgt ccatatcaaa
     1141 tataactgct tttatcattt gttgaccgct actatcgcac tgcaaccgtc aagatgaaca
     1201 cttcctgaca gatctatctt tnnnnnnnnn ngtgataatg tccgtatatt caccgccgat
     1261 accggggttg aagtcaaaag gattatcatc tatattcagc agtgttacaa cggtttcacc
     1321 gtcacagtcg cgcctgaatg agaactgtct gttaagaagc tgtaggggcg tgtaatcgcc
     1381 gtgagcaagc gcctttgaag atgtacgcag tttgcacagt gcagcaatct cacctgtcag
     1441 gtcggcaaca ccctcagcct tgaacttttc ctcattatat tctacacgaa gagcatcgtc
     1501 accttggtgc ttatcgccct ctataccgta ttcactgccg taataaacac ccgggatgcc
     1561 cggcattgtg aacagaagcg gatagataac aggaagctga cgcttgttgt tcagcattgt
     1621 agcaatacgt gaaacatcat ggttgtcaac gaagctgtaa agaagctttc ctgtataaag
     1681 gcaccactgc tcttttccaa actgacgatt gagtgaatgt gctatctcaa acatattgag
     1741 atcattgaag ctggaaaata agcccttgta gcattcgtag ttcgtcacgc tgtcgagcat
     1801 ttcggaattc atccagcgat tataatcgcc gtggagcgtt tcgcccatca gccagaagtc
     1861 gtttttaagc catttgcaat gaccgtggag ttccttcaag aagttgagat ccaggcaata
     1921 tgcaac
//
LOCUS       DAWAOG010000423         1924 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7820_length_1924_cov_2.090423, whole genome shotgun sequence.
ACCESSION   DAWAOG010000423 DAWAOG010000000
VERSION     DAWAOG010000423.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7820_length_1924_cov_2.090423"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    743..752
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          488 a          442 c          487 g          497 t
ORIGIN      
        1 gtcagaacgg cgaattggga gtggcgaccc gccacacggc gaatttgagg cggctcacag
       61 ccgcttattg ggtctttttg cgcaggagga aaccgagaag cgagaagacg aggaacattc
      121 cgaggtttgc gattactaca cttgcaccgg cggggaagtt gtcggcatag taggttatcg
      181 ctataccgat aatgtagctg acgcaggaaa cgatcgcaga gcagatcgtg acactgcgga
      241 agctcctgaa tactctcatt gaggtcagcg cagggaagat tataagactt gatatcagga
      301 gtgcacccat aattctcatt cctaccacaa tagtaagggc ggtgaggagt gctattatcg
      361 tgttatatac acctgacttt acgcctgtcg ccttggagaa attttcgtca aaagtcacgg
      421 cgaaaatctt attgtagaat aacacgaaca ttacaaggac gataacagaa cagattatgc
      481 tgagtgcaag atcgtcattg cttatcgcca taatgctgcc gaacatatag ctgttcatat
      541 cggtcgcact gtttgtgccg gacacaacta tcgcaccgat agcgagcgca ctgcttgaca
      601 ggagcgctat tgcggcatcg ccttttattt tgccgtcaga gcttattctg agcaggataa
      661 acgcggccgc tattactacg gggatcgata cctgtaacgg cgctaagttc agagcggagg
      721 cgatcgcaag tgcaccgaaa ccnnnnnnnn nngggaaagt ccgtcaccta tcattgaaaa
      781 acgcttcaat acaaggctta cgccgagaag tgcggcacaa agcgaaacga gaagtccgac
      841 tacaagagcg cggagcagaa atccatattg aaaaatctcc agcattttaa cgtacctcct
      901 ttgagcctgt gtggtggtcg caggtgcagt gacagtcaca ggtgctcatc gcgtggaata
      961 tgtcggtgct acggtactcg gctgtagtgc cgaagaaata tgcaccgctc tccatatgga
     1021 gtatctttgt accgtatttc agtgcgcctt gcaagtcgtg ggagatcata attatcgtta
     1081 tgccctgctc acgattcagc ctgcttatta tctcatacat ttcagcggta accatagggt
     1141 caagtccgga aacaggctca tcgaggagaa gaagttttcc tgtcgcacaa agcgcacggg
     1201 caagcagtac acgctgctgc tgacctcccg aaagattgcg gtaactgcgg tgggctatgt
     1261 gggttattcc gagcagttct atgttatcgt cacactgctt tttgtcggct tttgaataaa
     1321 acggagaaag tcctttcctg ccaagacagc ctgaaagcac gacctcgcgg acggtagcgg
     1381 ggaagtcttt ctgtgcggct gtctgctggg gaagatagcc gatctcggtc tgctttaatc
     1441 cgtcaccgaa gattatttcg ccacagagcg gggaacgcag tccgagaagc gttttcataa
     1501 gggtggattt accgcttccg ttctcgccga ctatgcacag ataatcacct gcggaaacat
     1561 tgaagctgag gttctttaca acaggcattc cctcatagcc tgcggtaaga tttttgcatt
     1621 ctatcaaata tgacattgta tcaactcctg tcagccgtta agggctactt tcagattttc
     1681 aaggttttgt ttcataaggt caacatagct tatatcgttt tcaagatcct cttttgaaac
     1741 gttgtggcag gagtggagca taagcttttt cgctcctgtt tcatcgcaca aagtatttgc
     1801 tatcttggtg ctgctaaatt cgaggtaata aactgcggga atgcggtttt cttttatctt
     1861 gtctgtaagc tttatcatag tcgaaacgct cggctcactt tcatctgagc aggcgctgaa
     1921 tgcg
//
LOCUS       DAWAOG010000424         1921 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7832_length_1921_cov_2.223473, whole genome shotgun sequence.
ACCESSION   DAWAOG010000424 DAWAOG010000000
VERSION     DAWAOG010000424.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7832_length_1921_cov_2.223473"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          508 a          470 c          362 g          581 t
ORIGIN      
        1 atataccgct gtcttgttac cgataagctt atcaaagtcg ccgtacagct tgctttccgc
       61 aagtgcgtca agtgccgccg taggctcgtc aaggactact acaggcgcat ttttatataa
      121 cgctctcgca agtgccagct tctgcttttc gccgcccgac agatcaacac cgtcatcgta
      181 aacgatcttt agtatctcgg tatccatacc cttttcaagt tctgagagct tattctcaag
      241 tcccgaatca ataacgcact gccttgcctt ctccttatcc gatttatccg cactgctcat
      301 tgaaacgttc atatacagag gaaatgcgaa cagctcgacc tcctgaaaag caggcgagaa
      361 tatcttataa tagctgtgct tgttatactg ctttatattg actccgttca gcagtatctc
      421 accctccgtc acctcataca gccttaacag tagctttata aaggtagatt ttccggcacc
      481 gttaagccct accactgcaa gccgttcacc cgcgttaaga gtgatattga cgtttttcag
      541 cgcatatttt tcagccttgg gatatttgaa tgaaacattg cggaaagtaa attcatagct
      601 gtcacactgc ggaacatcaa ttcctttgtc gtccttatca ccgcagtctg cgtccataaa
      661 gctgcggaaa tcgtcatatt ccctgctcct tgcaagtaag tccgatacag cggtaaggag
      721 ctgatttgta taatcaaaga acgtcattga cgatgcaagg tacaaagtga aattacctat
      781 agtcatactt ccgtttacaa cgctgtacaa gagccatgcg taaaccgccg cctgcactcc
      841 tgcccatagt acagcattta tgacagccga caccgcccat atctttgaat ttttcttctg
      901 cgccgcataa cgctctttgt tcagcacttt gaacttttct gtaagccaat cggctattcc
      961 gaacattctt atatccttag ccgcctcaaa gtcgcttgtg gtataagaaa gataattgtg
     1021 tctgcgccac cacggagcta atggatccca cacctttttc ttgcagtaag cgtttgtctt
     1081 gttggtgaca acaaattgca gtacagagca gataagtata gcaataacga taaaaatatt
     1141 catcgtaccg agaatggcga taccgacaac gattatcggt atgagcgtta aaagacgcac
     1201 aatgcctctc atcattccct ctatgccctc attattgctg ttgcaggcgt ttcctgcttt
     1261 ctgatagcag tccataaagt ccttgtcctc aagatagccg taatccaccg acattatctt
     1321 gcggttctta agtcccatct gcctgaatct tgccccgata taaatgtgaa agaatgagtt
     1381 gccgtaaagg ttcagcatag tagccgtaat ctgtattacg gtaaatccgc ccataagcag
     1441 tgcaagcgca gtaacgctct gacctgaagt tatcatatca ataacgaact ttgatatgaa
     1501 cgaccacaga tactgcatta tcggcgcaca gattatgctg attattataa caggtataaa
     1561 cctgtgcgaa tagcttagca ttgattttaa tgcgtgaatg ctgttgctga aaacgccgta
     1621 ttccttatga taaggattgg tgtttttctc tttctctttc tctttatttt tcttcatttt
     1681 tctctcccac ttttataaaa ctcatgtcct ttcggtcaaa gaaaggataa tcacggttgt
     1741 ttacgcttgt ctgataacca tgcggctgat tgagtacggc aaacgcacct gtcagcaaaa
     1801 taagcatttc cggcaagtag tttcttactc cataaacttt ttctttatca ttgtcggcgc
     1861 aaagcacgct tatttcgatt atctctttgt ttgagccgtt gatgctcaaa agatactgaa
     1921 g
//
LOCUS       DAWAOG010000425         1909 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7890_length_1909_cov_2.192557, whole genome shotgun sequence.
ACCESSION   DAWAOG010000425 DAWAOG010000000
VERSION     DAWAOG010000425.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7890_length_1909_cov_2.192557"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1114..1123
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1526..1535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          589 a          364 c          454 g          482 t
ORIGIN      
        1 tacgaggtca agatcgatat tgatgaagag ggtaacgtat tcatctgcgg tcaggatctt
       61 gaaaaggctc agaactgcat agcgatgata aaggctataa catctcctcc ggaggtcggc
      121 gctatttaca agggcaaggt agttcgcata atgaacttcg gcgcatttgt tgagattgct
      181 cccggcaagg acggtatggt tcacatctca gagcttgaaa acagaagagt tcgttaaggt
      241 tctcgacatt gacaatcagg gtaagatcag actttcaaga aaggctgcac ttgctgaaat
      301 cgaagccaag aagaaaaacg gctgataaaa gctcagaatt gataaaacag acctgtggga
      361 tactcccgca ggtcttttta tgtgcatttt cggcttgcaa tgacacgttg tttgtgatat
      421 acttatactg tatcattatg cgataacagc gtactttgct gtatattttc agctcttttg
      481 cagaaaatat caagagaaaa tttcagcaaa ggagcaggtt tatgaataaa gataacagca
      541 taaatgatga acgaaccgac gaagaaaatg aagagcaggt aactgcggat cagccgtctg
      601 ataacggcgt attcgcttct gccaatgcgg agcataacat acactgcctt aatgtcatag
      661 gtcagatcga ggggcactat atcctaccgc cgcagaacaa aacaacaaaa tatgagcata
      721 ttatgccggc tcttgtggct attgagcagg atatgagcat agagggactg cttataatac
      781 tcaataccgt cggaggagat gtcgaggcgg ggcttgccat atcggaactt atcgcaggaa
      841 tgtcgaagcc cacggtttcg atagtagtcg gcggcggtca ctcgataggt gtaccgcttg
      901 cggtaagtgc aagaaagagc tttattgtgc cgtctgcaac aatgactata cacccagtca
      961 gaatgaacgg gcttttgctc ggcatacctc agacgttatc gtattttgag cgtatgcagg
     1021 agaggataat caactttgtc agtaagaaca gcgatataaa gcccgaacgt ttcagagagc
     1081 ttatgatgaa gaaagatgaa cttgttatgg acgnnnnnnn nnngcggtgc agtgcctgta
     1141 ttcgcttatc gaaaataacg gctctgctaa aagtaagaaa gatacagctt cagatccgac
     1201 tgcagataaa aagaaaaccc cttcatcatc aaaaaaatca gtacgaagca cacgggaaaa
     1261 ggggaaagtt acagctaagg tcagcacttt cagggaatat cacccgggtg gtgcggaagc
     1321 taagtctatg cggggtactg caaatgtttc agaccgttat tgatgaatac gaagtgttcg
     1381 gcggcgctat gcagtatgat aacaatgcct attatgccat agacggctgt gtgttttcat
     1441 acagtataaa tgctgatgca aatcataccg agctatcgac tgagcccggt tcatatctca
     1501 ggttcacgga aatatcgatt tagtcnnnnn nnnnnttccg ggcttatgcc cggacatata
     1561 atataataac atgccggaag gcactacaga ttggaaagga acaaaagaaa aaatggagta
     1621 cataagtata attattcaag gcattatcca gggacttacc gagtttctgc cggtatcaag
     1681 ctcgggtcac ttatctgtgg cacaacattt tatgaatgtg ggcgaaaaca gtcttataac
     1741 ctcggtaatt ctgcaccttg gcactctgct tgcggtattt atagcattct ttccgactat
     1801 atggggaatg ataaaggagt ttttccttac aataaaggat atattcacag gaaaattctc
     1861 atggaagaat atgaacgcaa acagacgtat gatgtttatg gtcataatc
//
LOCUS       DAWAOG010000426         1908 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7899_length_1908_cov_1.730167, whole genome shotgun sequence.
ACCESSION   DAWAOG010000426 DAWAOG010000000
VERSION     DAWAOG010000426.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7899_length_1908_cov_1.730167"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    257..266
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1342..1351
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          422 a          485 c          405 g          576 t
ORIGIN      
        1 actgagatta ggctgtccca taaatagatt gtttgccgcc tttgtgatcg aaccggtttt
       61 ttcgatctca accgcatatt taagatgcag caggttcatt gaaaaccata tcctttcatc
      121 acagagggaa aacctctgtt aaattataac ccttatatat tacagtctgc gaaaaagact
      181 ctttttttat aaaaatggag caacttctta aaccctatcc ccaaacccct tccccacggg
      241 aaggggctga gtgtggnnnn nnnnnnctca ccccgcttgg ggctacgccc caaaccccgt
      301 tataaataca gtttttgaca gtctgtatat atttatttta ctttttaggt tcaacgtgtg
      361 taaaagactg tatcgcctct gttatagtct tttcgtgtgc aagcttgccg tacttgtcaa
      421 cctctgcctt gttcttttcg ccgtgtgtca ggcagcctgc tcccccgatg cagcctccga
      481 cacaagccat accctcaatg aagttgttgg gcagaagatt tttagatgca cgcataagtg
      541 cacgcttgca ttcttctata ccgtcacagg acagaccgtt cagcttgaag tcctcgttgc
      601 cctgttcttt aagtgcttct gcgactgcat cggcaagacc gccgcaacgt gcgaatattc
      661 tgccgtagta cgaagcgttg tcaagctctg ttccctcaag ctcggatacc tctatttcct
      721 tactgtcgaa gatagcctgc aattcttcaa acgtcattgc ccagtcaaca tagggtttta
      781 ccttatccca ctgtacttcc atcttctttg cggtacaagg tcctatgaat actaccttgg
      841 ctgtaggatc gctgtccttg atgaactttg ctatttcagc cataggtgag aggttgtgtg
      901 aaatcttgtc gaccatagtc gggaacgctt tctcgatgta cattacgaat gcgggacagc
      961 atgagcttgt aaggaagccc ttttcggcaa gctcctttga ctccttgtat gctaccatat
     1021 ccgcaccgag agccgcctca acaacgctgt ggaagcctaa ctgcttgata gccgttacga
     1081 cctgatccag ctttgcatag ctgaactggc tggcgatcga gggagctaca acggcatata
     1141 ccttgtagtt ctgattgttg ttactgccct ttaatgtttc tatgatgtca acgatatagg
     1201 acttgtcgct tattgcgccg aacggacagg tataaacaca cgcgccgcat gaaatgcact
     1261 tgtcgttgtc gatatgagcc gccgccgagc ttgtgtctgt tgctcttgag atagcgccta
     1321 tcttgcaggc tctctcgcac gnnnnnnnnn ncaatatagc accgtaagga cacactttag
     1381 cgcactgacc gcagtttacg cactttgact tgtcgatatg agccttctgg tgctcatcaa
     1441 aggtgatagc gcccatacgg catacatctt cacagcggtg agcgatacag ccgcggcacg
     1501 cctcagttac atcatagccg ccgacaggac attcgtcaca ggcaatatcg atgacctcga
     1561 tgacattggg gtttgtaggg tcgccgccca tagctatctt tatacgttct gaaacgatcg
     1621 ctctttcctt gtagatgcaa catctcatag taggcttatc cttgacgatg gtctttgcaa
     1681 cgtcattgaa aaagctgaag gaatcggcgg gcttctccgt gccctctgtt gtccatgcgt
     1741 gttttgcaac ttctcttaag accttgtact ttaagtactg taccttggtg tcgaattttc
     1801 ttaacttctt gtccattttt atttctgcgg agtttcccgc cgaccgcctg tgcggtcgct
     1861 cctttcatat agttttttct gtttgtccta cttttttata tcagcttt
//
LOCUS       DAWAOG010000427         1906 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7913_length_1906_cov_1.914100, whole genome shotgun sequence.
ACCESSION   DAWAOG010000427 DAWAOG010000000
VERSION     DAWAOG010000427.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7913_length_1906_cov_1.914100"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1498..1507
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          549 a          454 c          305 g          588 t
ORIGIN      
        1 tcacacatag cagcaacgtt attaaataga atagcgaacc cgagaagtgc tatcctacaa
       61 acagaactat cggtagcctg ctatcaatgt taattgctaa aactgtataa cagaagtaca
      121 ctaaaaacaa actttctcca catatcaaaa aaagcgagcc gagcgtacta tcctttgcgc
      181 ttcgactcac ttttttcggg ttgcatcaca acccctttac tttatttaca ttgttttata
      241 tcagtcagca ggaactatat aatagatacg ttcctccggt ttagcataac caagcttatc
      301 ccttgcgata gtttcaatgt actcaacttt atagctgtct tcagaatatc tgccaagcat
      361 tttgttatca ttttccactt cagtcacctg cgcagtaagc tttgcaagct cttctttctt
      421 ttgtgatatc tccacctgta tcgaaatgaa gttatacaca catatcacaa gcgccgttat
      481 caaaagaggt gcaagtatca gcataacttt attgaatttt cctgattttg ccttcaatcc
      541 tgctgacatt tacaacttcg tccccttcaa gcttattatc attattatat aatattctgc
      601 ggtgcttttt caaggctgct tctgctcttt ttattttatt attcataatt ttgtaaaaat
      661 gcacaaacgg aaatgctatt ttgtgtgcaa atgatacaaa cactttcaaa acgggtttta
      721 acagtttacc aaacacaatt ttaactatct ttattgcagt ttttataacc atggtatata
      781 ccaattttac aagccgaccg accgtaagaa aatatactat agctccgaca gctgcagaaa
      841 acagatatga cgcacggaaa attccgtttc ctatttccat cgaaagacca aaaagcacaa
      901 gaccgcaaag agcaagataa aacgtatcct ctaccccgac aataaagctg tggtgcggta
      961 tcacagtcct taacaccctc agtacttcat accccgcacc aagaaagatc ccgcatacag
     1021 aatagcacgc cagttgccag ataagcgatg tttcaacgtt catatgctca cctctatctg
     1081 aatatcctgg atatgaaatt atcggcacac tttgttctgt tatcgccgaa atggattgaa
     1141 catattttgc ctctgatctc tacgttaccg cttccggtaa aggctctttc cacctccagc
     1201 tccgaacctt taatacgcat attcccgaga acagtatata aaataatatt ctcgctgtca
     1261 tatgagatta tttcaagcac accgtttacg catagatctc tcctgtctgt aagagtcagc
     1321 atctgttgtc tgtcgttcaa aaaaataccc ccgctcatta tttacattac agtttagtaa
     1381 ataatattcg gggtatgtcc tgattatgct caaactactt cataaaggtc agaagctcca
     1441 tctttaccga ctacttcaac aaccttcagt acacgtattt tcagcgggct tttgcccnnn
     1501 nnnnnnnacc ttaacatcat aagacgctct ggcaggtttg ccgttcactg agatcttttc
     1561 ggcatcacaa gcttcgttgg ctactgttct tcttttgata aggcgtgata ctttcaaata
     1621 tttatccaat ctcatagaaa ctcctttcgc tgatcagtaa taaaaaacgg aggcggtaat
     1681 aatactgcct ccgctattta atcagattta attacttagc aactgcgtcc tttaatgcac
     1741 ttccggcctt gaatgcggga accttagaag cagcgatctt gatcttgtca cctgtacggg
     1801 ggttaacgcc ctcacgagct gcgcgctcgc gtacttcgaa tgtaccaaat ccaacgagct
     1861 ggatcttttc gccctttgcg agtgtttcgg aaatgctgtc tactac
//
LOCUS       DAWAOG010000428         1905 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7921_length_1905_cov_2.012973, whole genome shotgun sequence.
ACCESSION   DAWAOG010000428 DAWAOG010000000
VERSION     DAWAOG010000428.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7921_length_1905_cov_2.012973"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1042..1051
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          516 a          383 c          408 g          588 t
ORIGIN      
        1 ttcaatcact tcctttttct gaatgcccga tgccgctata acatggtcgc ggtcacatat
       61 tacagttggc attccaccga ccttataaag tacatcggca tattgtcccg cagtacttga
      121 aatttcgccg acaggggagt attttctgaa tattacctcg ccgtcaacgc ttgtgtatat
      181 ctcaagcggt gtgccctcgc gtattctcat ggttcttctt atttccttag gtattacgac
      241 tcttccgagg tcgtcaatac gtcttacaat tcctgtagat ttcattatat agatattcct
      301 ttctgtctta agaaactgtt ttttgctctt gcaaacatat tattcacagc aaagtgtaaa
      361 ttatacggaa atttgtcaca tattgtgttt ttagcttgct taattctatt tatcatgctc
      421 aataagaccg tatttatgat gttgattttg atcctaaagt gtagtataat aaatatcgtc
      481 tttcgggagg tgttacgatg acggctgatg aagagtatat gacagtttgt atagagcttg
      541 caaaaaaggc tgcaaagatc ggtgaatgtc ctgtcggagc tgttgtggta aacaaggacg
      601 gagatgtgat cggcagaggt tacaatatgc gtgaaacatt gcagtcaccg accgctcacg
      661 ccgagattct tgctattgaa caagccgcca aagctcttgg ctcgtggcgg cttaccgatt
      721 gtacactata tgttacgctt gaaccgtgcc cgatgtgcgc gggtgctgta atcaactcaa
      781 gactgaaaag gcttgtatat ggagcgtttg acgaaaaagg cggggcttgc gcctcgctga
      841 tggaaatatt cgattatccg ttcaatcatc gacccatggt aagaagccgt gtactgacag
      901 atgactgtgc aaagcttcta actgactttt tcaaggggtt aaggtcatct gatgatgcct
      961 gaaaccgcct ttttgtatag cttgtcaagc aacacgaaaa atgctgttgt caaagcaact
     1021 gttgctataa gagcagaaat annnnnnnnn naaataactt ttttcatatg cttatccttt
     1081 cgtagcattg cttaatcggt gtttatgcga tcaagacgct tttcggcatt ttttataaga
     1141 cttaaaagtg cagtaccttg cttgtcatat tcggcttcga tggaatttgt ggttattgat
     1201 ggagcatcta cagcgtcatt tgcggcatcg gcggcagcac cgacctcaag acatatggca
     1261 gaatcgataa gatgaagagc cgatgacgta gatactccga aaaggctttc attgtttttg
     1321 ctgttagcgt tatatgttat cctgaacgct ttgtaaacag aaagtgtcag cattgaagaa
     1381 acactcttac tgagcttagt tgactttatc ttctgcaaaa gagaaaaaat aacctcaatg
     1441 ctgttggtta taagcttctt attaagagca agcatgatat caccggactt tgaatcgctc
     1501 gccgtgccac cctccggtat gtcgctttcg gtgataatac cgccttttga aacgggtgag
     1561 cgaccaagca gatagtctgt cgacacatta taataatcag ccgcttttac aaggaattca
     1621 agaccgcatt cacgcttgcc cttctcatag tgtgacagga gtgcctgggc aatgccgaga
     1681 tctgcggcaa cttgtttttg tgtaagtcca cgttctttgc ggagcaggga aaggatcctc
     1741 ggaaaatcgg tgttcattat ttgacctctc ttaatctgac aatattcaca aagtatatta
     1801 taacggagtt attccgtatt gtaaagcgtg attttataca aaaaatcaag tcggttttta
     1861 tgtaataact ccataataaa acaaaaatcg ccgatttcgg cataa
//
LOCUS       DAWAOG010000429         1899 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7963_length_1899_cov_1.832430, whole genome shotgun sequence.
ACCESSION   DAWAOG010000429 DAWAOG010000000
VERSION     DAWAOG010000429.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7963_length_1899_cov_1.832430"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          466 a          417 c          417 g          599 t
ORIGIN      
        1 gtgtagcccg ccttgtgagc catctttata gcttcaagcg tttcggaaac agagcctatc
       61 tgattaagct tgataagtat tgagtttccg caaccgagtg aaataccctt tgaaagtctt
      121 tcggtgttgg taacgaacag gtcgtcgcct acaagctgta ccttgttgcc gagttcggaa
      181 gtgagctttt gccaaccctc ccagtcctct tcatcaagcg catcttctat agagattatc
      241 gggtactttt caacgaggct tttccagtga gcgataagct gttcagatgt gaacttctgt
      301 ttgcatttgg gcaggatata ttcacccttc ttttcgcctt tccactcact tgaagcggca
      361 tccattgcta tcataaagtc tttaccgggc tcatatcccg cattcttaac agcttgaaga
      421 atgaactgta tagcctcttc atcgcttgca aggtcaggtg caaagccgcc ctcatcgcct
      481 acagatgtag caagtccctt cgatttgaga agtgccgcaa gtgcatggaa aacctcggcg
      541 cactgtctta atgcttcttt gaatgaagga gcaccgacag gcattatcat aaattcctgt
      601 acgtcaacag tgttagccgc gtgagcgcca ccgttgagaa tattcatcat aggaacgggg
      661 agtctgtttg cgcttacacc gcctaaaaat ctgtagagag ggatttcaag agaagttgcc
      721 gcagctcttg ccgcagcaat agaaactgca agaatagcat ttgcgccgag cttggacttg
      781 tcctttgtgc cgtcggcatc gatcatcgct ttatcgactg cgtaggtatc gcaggcatca
      841 acgcctctga cggcattgtt tataatcgta ttgatatttt ctactgcctt tgtaacaccc
      901 ttgccgccgt atcttgcctt gtcgttgtcg cgcagctcaa gtgcttcaaa ctcacctgtt
      961 gaagcaccgc tgggcgctgt acctcttgca acagtgccgt cttcaagata tacctcagct
     1021 tctgctgtag ggtttcctct tgagtcgagt atttctcttc ctattacttt tttgattgct
     1081 gtacaaatca ttgtaatcct ccgtttgccc gcttttgaaa ccaatgcacg ttgtatagta
     1141 tcggtaattt ttcagggtta atacttatcg ggcggttaaa ataggttagc ggtaactaac
     1201 taatgaaaat tatatcacac tgttttgcaa tagtcaatag gttttgagaa aaaaagttag
     1261 agaaaactaa cttaatgctg gttttatgcc ccaccgaata ccggaggggc ataggtttat
     1321 ttgactataa agtcgagctt ctttacgttt gtatcctgcg agaatgttat tacattgtac
     1381 ataaacagaa cgttttcgta atcttttgga tttacaccga tatttgacag atcgctgttt
     1441 atatatctga ggtcaagtac cgttatatgg ctatagtttt tagtaaggaa cggtattaga
     1501 gagtgcgcat agctgtcttt tattaccaac agatttcctt tatcatattc ttttgggatc
     1561 tcgctttcaa tatctatacg aggtacgttc tgaccgagaa atgtggaata tttgtccttt
     1621 acatcaagaa attcgcggaa atacagactg ttatatgagc tgtcgggttt tgcaccgctc
     1681 ttgtagacag aaacctttac ctgtggctca ttgttcgcca gtgcgtaaat gtcgatagtg
     1741 tcgggtgtaa tgctgttgtc gagtgtcttt gagtagagag ttcccctgaa ctcgttagaa
     1801 gcgtgctcaa cgctgaattt gtcttcgctg taggcagtga agttcatagc agatgcagca
     1861 gatttatatc ctatatatgc accgtaactc gtccagtga
//
LOCUS       DAWAOG010000430         1898 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7975_length_1898_cov_1.619099, whole genome shotgun sequence.
ACCESSION   DAWAOG010000430 DAWAOG010000000
VERSION     DAWAOG010000430.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7975_length_1898_cov_1.619099"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1014..1023
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          463 a          515 c          363 g          547 t
ORIGIN      
        1 cggtcgtctg tgaaagctta gccgcaaata taccctgaaa atatgtaagg agcgagttta
       61 caaggtacac acaccccagc gctataaggc agtataccat accgcttata tcgacattca
      121 gccgcttatc cgaaatggtt atcttgtcta tagcaagtcc ctgtaaatac ggtgctacaa
      181 ggtttaaaac tgtaattatc agcatgaccg ataacagcag tatcatcaga tacctgttct
      241 tgccgatata gcctacaagc cttttcagcg tgcccttagc attcttcggc ttttcagcca
      301 tcaacattct ttcgtgggga ggtggacctc ctctgcccat cattctcaca ggaggacgtt
      361 cgttagccat ttaccgtttc cccctctccc tgcttcatct gcgaatagta aatatctctg
      421 tagacatcgc ttgcttccat aagctcctta tgtgtgccga aagcacatat cgagccgttt
      481 tcaagtaccg ctatcttatc ggctctcata acgcttgcta ttctctgtgc tatcattata
      541 acggtaacac cgcttaaatt ctcacgcagt gccgtgtgaa gctttgcctc ggtgctcaga
      601 tcaagcgccg atgtactgtc gtcaaatatc aatatctcag gctttttgac gatagctctt
      661 gctatcgcca tacgctgctt ctgacctccg gacagtgacg cgcccttttc acttatcatg
      721 gtatcataac cgtcattgaa accgcttata aactcctccg cctgcgctat ctctgcggca
      781 gtctttacct cttcgtccgt agccgtttca cagccccagc ggatattttc tgctactgta
      841 ccggagaaaa gctcgctttt ctgtaatact acacctattt tgcttctcaa tgtgtcaaca
      901 tcgtaatcct tgacatttat accgtcaaca agcacatcgc cctctgtagc atcgtaaaat
      961 ctcggtatga gatttacaag cgaggtttta ccgcagccgg tagagccgag tatnnnnnnn
     1021 nnncatctgc cccttttcgg ctttcagatc aatgtctgaa agcaccggct ttcccgacgc
     1081 acccggatat gcaaagctga cattcttaag ctcgaccgta ccgctgttat cgccgtctgc
     1141 atttttctta ccgtcaccga taacagggtc tgttgaaagc acttcattta cacgcttcat
     1201 agacgctgtt gcacgcgaaa cctgctggaa catcatacct accatcatca ccgacataag
     1261 tatctgtgtt atgtaagtca cagccgccat tacatcgccg acctgcattg ccttagcatc
     1321 gacctgaaaa ccgcctatca taataatggc tataaccgaa gcattcatta ttatcataag
     1381 tatcgggttc agcgttgcga gcagcttctg aactttcagg tttgttgaca taaggtctgt
     1441 gtttgcgttg tcaaacctgt ttatctcata ttcctcacgg gtgaaagcct ttactactct
     1501 tgctcccgaa acgttttcct gtaccacgct gttcaccttg tcaagtcttg actgaacgat
     1561 gccgaaaagc ggcgaggctt tcttcataat tatcgcgacc gcaataagct gaacaggcag
     1621 tgagataaca agcactatgc cgaaattcac attaagtgtg agaattaccg caatgccgcc
     1681 tataaactgc attcccgttc ttacaaacat tctcagcgac attgccacaa actcctgtat
     1741 cgccattata tcgtttgtaa gtctggtaac aagtgagcct gtggtaaacc tgtccgtctg
     1801 ctgaaaagac atatgcatta cctttgcaaa tgcgtctttt ctgagatcgt tacccatctt
     1861 ctgagccgcc gccgtgccga atgccgccga ggctatac
//
LOCUS       DAWAOG010000431         1894 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_7991_length_1894_cov_2.077760, whole genome shotgun sequence.
ACCESSION   DAWAOG010000431 DAWAOG010000000
VERSION     DAWAOG010000431.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7991_length_1894_cov_2.077760"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          328 a          510 c          401 g          655 t
ORIGIN      
        1 atttcaagca gctcgtctat gccctcgcct gtctttgcag aaacgggaac gcagataaca
       61 tctcctcccc actcttcgca aacaagaccg tgctcggtga gctcctgctt taccttgtcg
      121 ggatttgcgg cgggcttatc catcttgttt atcgcaacta ttatcgaaag gttagcagcc
      181 tttgcgtgat ttatcgcttc taccgtctgc ggcatgatac cgtcatcagc cgcaacaacg
      241 agtattgcta tatcagtagc cagcgcacct cttgcacgca tagctgtaaa agcttcgtgt
      301 ccgggcgtat caaggaatgt gatatccctg ccgcctgcct ttactctgta agcaccgata
      361 tgcggtaacg tttgcgtttc ttattctgtc aagaagcgat gttttaccat ggtcatcatg
      421 tcccataata acaacgacag gtgaacgtgg ctgtaagttc tcgtctgtat cttcaacttc
      481 ttcaaacaga cgttcttcga tagaaactac tacttccttg gttacctttg caccgaactc
      541 ctcggcaacg atacaagccg tatcatagtc tatggactgg tttatcgtaa ccattacacc
      601 cagactgaac agcttcttta taacatctgc ggctgtaact ttaagacgtg acgcaagctc
      661 cgatactact atctcatcgg gaacctgtat ctcaagctgt ttctttcttg ccttttcaag
      721 ctcaagacgc ttcagcttct gagcctcggt ttccttcttc ttgctgaact gctgtttgcc
      781 cttctgcgac ttctggctta tcttctgctt ctttgtgtaa gcgtcgttct gtcttctgtt
      841 tccgccctct gtagacgcaa tcgtttcgta cttctcattg tacttatcga ggtttacata
      901 actgcctctt gtatcaacgt gctttgtgac ctgttcgcct ctctgtacag cttcaccctt
      961 gacgtgcttt gtgggaggtg catttgtctt gatatttgca aggtcgataa cgggccgcgc
     1021 gggagctgtc tgctgtgcag gctttgaatc cctgcggtcg tttctgccct gatagccgtt
     1081 tctgttgtcg tttcttctgt cgttgtttct gtcaccatag ggctttcttt cgccggactg
     1141 tctgtcgtta cggttgccgc cgcgatcatt gttgcgatcg ccgtaaggct ttctgtcacc
     1201 ctgcggtctg tcattacggt tgtcattgcg gttattgttg cgctcgccgt ttctgtcacc
     1261 gtatgacctt ctgtcattgc gctcacccgc aggctttctg tcggcaaacc tgtcattctt
     1321 tgcttcgggc tttgcttctg cctttgtttc aggctttgct tcgaccttag gctcagtctt
     1381 tgcttcgggc ttgctgtcag ccttgacttc ctttttgggt tctgccttgg cttcggtctt
     1441 aggctcagtc tttgcttctg tgggcttagc ctgtgccttt tccgtctttt cgggcttatc
     1501 gtcagcctta acggcttcct ttttgggttc gggcttcttt tccgccttct ttgcagactt
     1561 tacctcgggc ttttcttcct tcttttcagc cttgctctta cctggttttt ctgcgaatac
     1621 ttctcaagta cgaccgaaag ctcttcttct gaaagcgaag cctgcgattt tttctgtacg
     1681 ccgaaatatt cctgtacaac ttctgtaatg tcgtttgttg aaacattaag gtcttttgcc
     1741 agctcgttca cacgatattt tttatccatt cactgatccc ctttttggtc aaatttagaa
     1801 ttgtatatat aaatcggatc taccgcttat ttccctgtgt gcctgcccgt aagacttacg
     1861 aacaagccct cgtcgagaac ggcaagtact cccg
//
LOCUS       DAWAOG010000432         1885 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8050_length_1885_cov_2.475956, whole genome shotgun sequence.
ACCESSION   DAWAOG010000432 DAWAOG010000000
VERSION     DAWAOG010000432.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8050_length_1885_cov_2.475956"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1628..1637
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          508 a          431 c          384 g          552 t
ORIGIN      
        1 cggtttatct ccgcaaagca aggaacggcg atgcagactg ttattattgc cgctgtcaga
       61 acggctgtta ttttctttat catctgtcaa taccccctta cttcaaaaag agaagccacg
      121 ctgcgccgaa agtcaataaa gcggctatca gcgcgatcag tcccgtccag cgccaataag
      181 cgcccttatc cataggcaga tcgccgacaa actttcccgt ctgaccgttc attgcaaaat
      241 tatatacttt gccgttccat tttgcggcta aaagccatac agggtaaagc gcatacttta
      301 cagtaccgtt tgcaagccgg accgatgaat tttcaggcgt taccgttgaa taacccgctg
      361 ttgtttcagt cagcttgtca agtgtgctct tttttactct gtcatttgct ctggcatttc
      421 ccgtctgtga gtcaacgtca tatttgtccg ccatatagcc ggacagataa gccgtctgaa
      481 acggcacttc accatctgcc gtaaacggct cgagcgactc tgtaagatca tctgccagct
      541 tcgacgaacc gtcaaccggc actctttcaa agctgagcga accgctgcgg aatagtctgt
      601 aatatgatgt ctttgtgtaa ttgtactttg aatcgctcca tgtttccact ttggtcgcac
      661 ggtaatacgc ctgtgcatcg gcatcggcat catacagcca aaaaggaaca tatacaccct
      721 tgacctcttc tatgtagctg ttgcttctga aagcctttgg cagaagcctt ttcttgcaaa
      781 gatgattttt aaagccctct tttgccgctt ctttgtcaag cttgaacggg ataattaaat
      841 ccgggcgctt gtcgcctgca aactgacctg tcataacaac gggacttccg caatatgggc
      901 attcggaagc gcctgttgtt tcgtcacaga cgatctgacc gccgcaggaa ttacagaggt
      961 aaaccttctg accgtcaagc tcccccttca tccattcgtt tccgggctga cttgaccagt
     1021 ccatcatgtc cggtttttct tctttgagga tatcatcgtt ttctttaagt gtttctgcgt
     1081 caaattcatt gtcacaataa ggacacttca tcttctgcac gtctgtatcg aactgtaacg
     1141 gtccgccaca gcacgggcac ttatattcga gtatagctcc catataatgc aatttccttt
     1201 cgtttttggt aaatatcata ctgatgtttt tagtatatca tataaacaga ggtcgtgcaa
     1261 tacacaaatg ggtaattttt tatattctgt gacgcgttac attacagacc ggtcgcttag
     1321 acaatgactc tttttatgtg ttgattgcgg cttgagtctg actttagcaa attgccgtga
     1381 tgtaaagact atattattgc attcaagaaa atgatgttcg gaaatttaat ttttatccga
     1441 agtaaaagac acgcaaagct acattgcgga acagtcggcg ctataaacaa aaatactttc
     1501 gcgtattttt gcggtgtgtg agtttattaa aaggggctgt gactcaattc ctcggtttat
     1561 caaattttat taaacaaaaa tgcaatatat tgtaaaaaag tctaaataat aaacatacag
     1621 ccaacatnnn nnnnnnncaa ggtgttaata atttcgtgag taaatcagcg caactgcact
     1681 gtcaccgaaa ttttcaaaag gtgaaatatc ctgagaatac acagttttgt gtataacttt
     1741 tacggttgat agttgacaga ttataaactt ggtgaatggt tagaagttag tgcaacaggc
     1801 agggcgagca aggcaaggac gccttaaacg tgtgtgccca aagtttcgca aggacgcgaa
     1861 accatacaag cacacacagc accgc
//
LOCUS       DAWAOG010000433         1885 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8053_length_1885_cov_1.896175, whole genome shotgun sequence.
ACCESSION   DAWAOG010000433 DAWAOG010000000
VERSION     DAWAOG010000433.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8053_length_1885_cov_1.896175"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          547 a          422 c          403 g          513 t
ORIGIN      
        1 actgcggaga tgttaacctc tatgtactct aatccgtact gctttatatt gctctgttgt
       61 gcaaaacgac agacattttc aaaaacgatg ttgccgaggt ctgaaataag ccccatgcgc
      121 tctgccatcg gaataaagat ctcgggcgat ataaagccga gcgtttctct gtctttgagc
      181 cttacaagcg cttcggccga tacaaacttt ttctttgttg ttgaatatat cggctggtag
      241 tatacttcaa gtccgttatt cttgactgcg tactgcagca gtcgttctac accggaaagg
      301 taatttattt catctataac agcttcgctg accacgagaa tagaacgttc ggatctcatc
      361 tttgcaccga gtatatgcgt cataagctcg gtaattttat ctacgctgtc ggcacacttc
      421 gggcactcga taaacgaaag tctgaaagag gtattgaccc tgacgcctgc cgatgtcgaa
      481 aattctgtct tgtcaaaatc ggatctgatc agcttctcac agcaagcttt gtgttttacg
      541 ataaagctta tgctattgtc ggaggtgtga taataatccg aacgcagaac agtcgtcagc
      601 ttaagcgctg cccagccgag aatattatat acctcgctta cgccaacaga atttttaaga
      661 agctcggaat ttgcaagtgt gaatgtgaca acaaagaagc ctttaccgtt ttcacaccac
      721 tccttgagca tcatttcaaa tgcgtgacgg ttcagcgcgc cgcttatatc catatcgttg
      781 tattcgtgcg ggttctcgac cgacagaaaa ataaacagct gcataagcga aacgcccatc
      841 gattcaataa gccatgtggg attgaacact tgcactattc cgattattat ccatcctgat
      901 attccgcaaa gtgcggtaaa cattatatgg ctcggtacgg atttgcggcg gcggataacg
      961 gtaatgatcg tccacagtac gtacaccgca aggcaagcgt aaagcatcga aacaacaggt
     1021 ccgtacgaat atctgccgtc actgcctata tgataatcta tcctgccaaa cgcagtaatt
     1081 acaagcgaaa gcaaagccgg caggattctt atgataagct ctatcggctt gtaacgcttc
     1141 tgctttctgg tcaggatatc gatatagata aaaaggaaca tcaaaatcaa tacaagtgag
     1201 ccgataaaca gctgatgcgc caggcggttt atgaacggat ctattgtatc aatatgatac
     1261 agggtataaa tggcacagcc ttcaaaaacg ctgtagaaca gcgaaaccga aagataaagc
     1321 gtatatatcc ttgtggacag aagctttatg tgcttattca aaaaatagat aacggcgatt
     1381 gtcagcataa cgccgaacga aacgatctga atacccagat tcatatatac ccctccgcac
     1441 tgttaataca ctaattatac gttcaaaaca gcaaaaagtc aagaaaaaga ccgcccttgc
     1501 gggcggtcgt gtgatgtaaa agcttaatta tgcaaccata gaacctgcta caagctgtac
     1561 aagcataaag catacgatat cggggttctt aatattctgc atcttttcga tgagtgtggg
     1621 taagttagcg gatatttctg tgctgaaagc ttcaagagcg gcactgtcgg aaatatcaac
     1681 agaatttgag ctgttgatat cggggagagc ttcctcggta tatgccgctt ccttgctctc
     1741 aacctttaat gtgaacatat ctgcgccgtt tgaagcaact gcgatatcaa atgttccgtt
     1801 attgccgtcc atagcaacat tcagcttgac ctcaaaggtt tcgttgtcat cggtcgtaac
     1861 cttgaagctg tatgtaccgt tgccg
//
LOCUS       DAWAOG010000434         1884 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8060_length_1884_cov_2.956807, whole genome shotgun sequence.
ACCESSION   DAWAOG010000434 DAWAOG010000000
VERSION     DAWAOG010000434.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8060_length_1884_cov_2.956807"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          589 a          357 c          501 g          437 t
ORIGIN      
        1 agtataggca attgtgcttt cagatattgc acaagcctta aaagtataac gataccgaac
       61 agcgtgattg agataggcag tagtgctttc agcggttgtt caagccttac aagtataacg
      121 ataccgaaca gtgtgacatg gataggcgat tgggctttca acggttgcac aagccttaaa
      181 agcgtaacaa taccgaacgg tgtgatgagt ataggcgaat atgctttcag aggttgtaaa
      241 agccttacaa gtataacgat accgagcagt gtgacatgta taggtgatag tgctttcgaa
      301 gactgcacaa gccttacaag tataacgata ccaagtagtg taacgagtat agctgctaga
      361 ctttttgaag attgtacaag ccttacaagt ataacgatac cgaacagcgt gacgagtata
      421 ggtgatgatg ctttcagagg ttgcacaagc tttacgagta taacgatacc gaacagtgtg
      481 acgagtatag acgattgggc tttcagcggt tgcacaagcc ttacaagcat aacgatacca
      541 gacagtgtga cggagatagg ctatagtgct tttgaaggtt gcacaagcct tacaagcata
      601 acgataccga acagtcttac gagtataggc tatggcgctt tcgaaggttg tacaagcctt
      661 acaagtataa caataccaaa cagtgtgacg agtatagacg gatatgcttt cggttactat
      721 tatgatgaag aagattacag ctccaaaaag atagataact ttaaaatcta ctgctacagc
      781 ggtacagccg gtgaaaagta cgcaaaggac aacggctttg actatgtgct tcttgacaag
      841 ttgcctacgc ttgcaaagat tacaggcgct aagctcggcg gcagagcggc agacgcgctt
      901 agaataaact ggactaagaa tgcttctgcc gacggttaca ttgttgagat gtatcagggc
      961 aataagtggg cgcgtgtcgg caagataaca aataacaaca caacaacatt cagacaatcc
     1021 ggacttaagg caggcactgt ttacaacttc agagttaaag catataaaat gagcggtaag
     1081 actgcggttt acggcgcata cagcgctact gttgcggcaa ggacaaatcc gtctgttata
     1141 aagggcgcaa agctcggcgg tagggcggcg gactcgctca ggataaactg gactaagaat
     1201 gcttcggctg acggatatat cattgaaatg tatcagggca acaagtgggt tcgtgttggc
     1261 aaaattacaa ataacagcac aaccacattc agaaaagcgg gacttaaagc ttcaagcgtt
     1321 tacaagttca gggtaagggc ttataagatg agcggtaaga ccgtgctcta tggtaactac
     1381 agcgctactg taaccgcaag gacaaacccg tcaatagtaa agggtgtcaa gatcggcggt
     1441 aaggctaagg acgctttaag agtgaactgg gctaagaaca cttccgcgca aggttacata
     1501 gtagaaatgt acaagggcgg aaagtgggta agagttgcta agatcacaaa cggcaacacg
     1561 actacattca gaaagtccgg acttgcaaag aatacaacgt ataagttcag agtaaaggct
     1621 tattatatga gcggtaagac ggcgctttac ggtggttacg gatatgtgag tggaaagacc
     1681 gcggcgaagt aacggctact gaataaaagt taataataag accgggcgtc gatgggaaaa
     1741 tcgttgcccg gtcttttgtg gtgggcggcg ggtcgccgct gacaattccg cctttaggaa
     1801 tgacggcgat cttcatagta aagttgaacg gcgggggtgc acgaagcgag tttgtagagt
     1861 gcgggtctgt gtgtgccttg cttg
//
LOCUS       DAWAOG010000435         1881 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8083_length_1881_cov_2.078313, whole genome shotgun sequence.
ACCESSION   DAWAOG010000435 DAWAOG010000000
VERSION     DAWAOG010000435.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8083_length_1881_cov_2.078313"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1148..1157
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          565 a          443 c          438 g          425 t
ORIGIN      
        1 ttctgaggct tccgaggctt ccgaagccgc cgacagcacg acagcttccg acacctcctc
       61 agaagcgtcc gcagaaaatt cctccgatac atcatcaaag cccgaaaagc ctgtcaccga
      121 ctttacgaca tatgtttcca acaccgttgt gtcgaccggc aacaatttct atatagaaaa
      181 agccgaaaac ataacctaca gagcatatct gcctgttgag cagtacggag agcttgaata
      241 caagttcttc tttacaaata tggttgactc gacctatgcg aagggcaagg atgcatttgt
      301 cggtaaagac ggcggcacat ataccgtgtc aaacgctgtt atcgcagacg gcggtacaag
      361 caccgaggac aaaatcacaa accgtacacc cgtaacattc ggcggtaaga cgacaaaaga
      421 ggtagcctcc gatgaaagct actggagcga ccctgttaca atgaatatcc ccgagggtca
      481 ttatcttgtg tgggagtgga cggtatccgg cgagggtatt ccatgcaaca agatgtcaac
      541 ccttacttcg accacaagct ctgccgacgg tgaaaccttt gcgtactgcg atgatatccc
      601 gctcccccag cttatcggag cgaagagagg ggttaagcat acagtagccg cgatcggcga
      661 cagcataacg caaggttgtc agacagagct gatgaaatat gagttctggg cttcaaagat
      721 atccgcacag ctcggaagcg atgtttcttt cttcaactgc ggtcttggct gggcaagggc
      781 aagcgatgcc gccgcaaacg agaactggct gtcaagagtg tcacagtatg acacggttct
      841 cgttgcattc ggcacaaacg atatcatttc aggcaaatac ggcggaaaga aaagcacagc
      901 ggaagaaata gaggaatatc tcgacacgat agtttcgtat cttacagaaa aaggctgtga
      961 tgtaatactg ttcaatgcac ctcctcagga cttcaagaaa acaaatgagg gcatcagaac
     1021 agcactcaac gagaaaattc cggcaattgc cgaaaaatac ggtgcaaagt tctttgactt
     1081 tgcttctctg ctctcgaccg cagatgagcc cgcaaaggct gtatacggcg gtcacccgaa
     1141 cggcgagnnn nnnnnnnata ccatctcatt tctgcgtttt tacagaatat cgttttttta
     1201 tacgctcata ataataacgc acagcatatt gataataaat atgccgaaca gcaaaacgga
     1261 tacagaaatc ctaatgatcc gaatgaacga aaggaaaaaa cggtatgaaa aagacagttg
     1321 ctattttagc ttccatagcc gctatgagct tatgcggttt atcagcatct gcggcagacg
     1381 gcgataacat cattgacggt gtgcttaacg gcgcagagga cattgtagac ggcgttgtaa
     1441 gcggtgcaga ggaaataatc actcccggcg gctcaacttc cgtcgatgac aacggagata
     1501 acgatacttc aatgcctgct actatctccc agccaactcc gggcgacagc acaaacagca
     1561 ccggcaatgt aaacacaggc gctcccgctt ttgagctggc ggcactcggc gtggcggcag
     1621 taggcggtct tacagcaatg gctgtaactg tacgcaagaa taacaagtaa cgaaacacaa
     1681 caaaatgccc ctgcttttat aagcggggga ttttgattat tgacagcgac aggaaaagtt
     1741 cactgaattt gctttaatta ccatctctgc cgtaaaaacg ctgtacaaca gataaaaaaa
     1801 gtagtataat gatatttaat gttgtgttgt cgggagatac ctttcatttt attacgaata
     1861 acattcggac tatttcaaat t
//
LOCUS       DAWAOG010000436         1880 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8089_length_1880_cov_1.972055, whole genome shotgun sequence.
ACCESSION   DAWAOG010000436 DAWAOG010000000
VERSION     DAWAOG010000436.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8089_length_1880_cov_1.972055"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1332..1341
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          496 a          486 c          408 g          480 t
ORIGIN      
        1 gttgatatac ttatgaagat ttacgttgat acccgaagcg tcagccgcac cgggattttc
       61 accgatagct cttacataaa gtccgagctt tgtcttgttg aggtagaaat atgcgagtat
      121 tgcgatgaca aatccgagcc atacatatat atcctgcgag aatatagcct caccgagaat
      181 gggtatattg ctgagaagcg gtatttctat cggacggaac gctgagctta cagactgggg
      241 tatctgttca cttgacaccc agccgccgat aagaccggaa acgcccgtac cgaatatcgt
      301 cagcacaaga cccgttacga cctggttacc gcggagcgtg acggttataa aggcatatat
      361 aagtgcgcct gccgcacctg ccgcacctgc cgccagcata gcgagcaagg ggtttcctgt
      421 agcgcacgca actgtaaagc ccagcgccgc acccataagc atcataccct cgacaccgag
      481 gttcatatga cctacctttt cacaaaggat accgccgaca atagcaaaca gtatcggagt
      541 acctgtctga atggctgtct gtaaaaacag acctatctgt tctaagatat ccattattca
      601 gcctccttta ccgcattgtg cttacgcaga gaaattttat agttgataaa gaactcacac
      661 gcaagaacga agaatatgat aacaccctgc atgatctcgg ttatagacga ggggatcttc
      721 attacctgaa gcgagcttcc gctctggaga agtatcgaga acataaatga cgcaacaacg
      781 ataacgggcg gcttcagctt tgcgagccat gccgtgatta ccgccgtaaa gccaagacct
      841 gccgacattg aatcggttat cgacttctca accgcgcttg cctgcataaa gcctgccatt
      901 ccgcaaagtc cgcccgaaat gagcaccgct atgatcatta tctttactgt gttcatacct
      961 gcgtattttg cggtcgtttc gctctcgccg attacatcta tctgatagcc gagctttgtg
     1021 tatttaagta atattgtcat tatgacaaca agcacaagcg tgattatcca gcctgcgtgt
     1081 atccctaaca catcgggcag taccgcattt tccgagaagt taggcacttt tgccgcaccg
     1141 agcgccgcag ggtctttcca cggaccgtat tgcagatagc ttataaacgc tgtcgctata
     1201 tagttcatca tcagcgtcac aagcgtttcg ctcgcaccga acttagcctt tattactgcg
     1261 gggataagcg cccataagcc gccgcagata aagcctgcta ttgccataag cggtaaaagg
     1321 aggatcggcg gnnnnnnnnn ngacacataa aagccataga tgccgcaccg aatgcgccca
     1381 tatagaactg accctcagcg ccgatattcc agaacttcat tctgaacgct atcgctatac
     1441 cgagtgacag tgtagtcagc attattgtct tgttcataac gtccttgaaa cgcttgaacg
     1501 gggtctgtat tattttgccg aaaaccttga aaggatcata gcccattatg gcaagaacaa
     1561 tgccgataaa tatcagcgac agcacaattg cgcctattct tatcaggaca tcgacaagct
     1621 tagacgtgcc tttctgccgc tttgaaatct gaatcatgct catacggctt tctcctctcc
     1681 cttattgata tggtcgccga ccatcataag tccgatatct tcctttgtaa cagtcgtggg
     1741 gtctactatg tcgattatct caccgtcatg tattaccata agtctgtcgg aaatgctttt
     1801 cagcacgtca agatcctcgc cgacgtagat aacggcaacg cccttctttt tctgctcggt
     1861 cattaagtta tagatcgtgt
//
LOCUS       DAWAOG010000437         1879 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8097_length_1879_cov_2.376096, whole genome shotgun sequence.
ACCESSION   DAWAOG010000437 DAWAOG010000000
VERSION     DAWAOG010000437.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8097_length_1879_cov_2.376096"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          455 a          497 c          363 g          564 t
ORIGIN      
        1 ttaagccttt tctctcctat aaaagtatac gcacaaggtg acggcgacat acctcttata
       61 aaattatata catcaattgc atttttcgag aagtctatcc tgcacatttc ctttgaaatc
      121 atacctgcat agcaagaaag actgtcgtcc tgaggtacag gctttatcgt accctcctcg
      181 caggccttca gtgtttgcac gataagctct ccgcctgtag ccgcaaggct gtcgtgaagc
      241 tctccaccgg tcatatccgg agcgatctca accgttctgc tcataagcat atcacctgta
      301 tcaagccctt ttgccataag cattgttgtg acaccgcttt tagtttcacc gtctatgatg
      361 cacttctgga taggtgcggc acctctgtac ttcggcaaaa gcgaggcgtg tatgtttatg
      421 cacttatact taggaagttc aagaatgctt tccggaagaa gctgaccgta agccgcaacc
      481 acaatacaat ccggggcaag ctctttcagc agttcgagcg acttttccgc atcctctccc
      541 tttctgagcg aaagcggctg atatacgggg ataccgtact tttcggcgca ctcctttacg
      601 ggaggtgcct gcatcttctt tcctctgttc ttaggcttgt cgggctgagt gaaaacagcc
      661 atgacattat gatgcttgtg taactcctcc agtgccgata cggcaaaatc cggagtaccc
      721 ataaatacta tgttcaaatg atttccccct tactttttac gttcctcaag ctgttcggaa
      781 gtcagcagtt catctgcctt gtcggtaaac aaaataccgc tgaggtgatc tatttcgtga
      841 caaagagcta tcgctaaaag ctctgtaccc tctatatcaa attctttgcc gtatctgtcc
      901 tgtgccttta cctttacctt tgcgggtctg tcaacatatc cccacatacc cggcactgaa
      961 agacagccct caagctcgtg ctgtttgcct ttcatagatg tgataacagg gtttataagc
     1021 tcgatttttc cgtgttcgtc gccgacatca ataacaacca ctcttctcag tacgcttatc
     1081 tgtggtgcgg caagacctac gccgcttgcc tcctgcatgg tttcatacat atcatcaagc
     1141 atcgtccaaa gcttatcatc gaagacagta acgtccttgc atctttttct gagcgactca
     1201 tcgccgtcct tagctattat tctctttgcc atttcaaaaa tcctttctgt tgtttatatg
     1261 atctcgccgt tcatatcggc gaagaagctt actttatcaa gagccggcga cttatatgca
     1321 tcaagcagag tcgcctgcat taccgacctg aacttcttat tgttcttgca tttaataatg
     1381 atcctgtagc ggaacttacc gtttactttt gcaacagagc aggcggacgg accgagcact
     1441 cttatcggga taccgtgacc ctcaagattt tgcataaaca gctttacaaa gctgtctgcg
     1501 ccttttttgc atatatgctc ttccgttgcc gaaagtccta tctcacatat atcgcaatac
     1561 ggcgggaaaa gaagtgcttt tcttatctcg cattcctgcc tgaaaaactc atcgtaattc
     1621 tggtccgccg caagattgag aacataatgc tccggggaga atgtctgcaa atacgccctt
     1681 cccttttttg aacctctgcc gcatcttccg accacctgtg ttataagcga aaatgtcttt
     1741 tcatagccga ggtaatcccc tgcatacagc gatttatcta tcatcagaac accgacaaga
     1801 gtcacattct caaagtccag tcctttagct atcatctgag tgccgaccat aatgtcatat
     1861 tcgccgtttt tgaacttat
//
LOCUS       DAWAOG010000438         1876 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8118_length_1876_cov_2.372323, whole genome shotgun sequence.
ACCESSION   DAWAOG010000438 DAWAOG010000000
VERSION     DAWAOG010000438.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8118_length_1876_cov_2.372323"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          559 a          366 c          419 g          532 t
ORIGIN      
        1 aataaaaata gtgcgggtag ccggacttga accggtacgg tattgctacc gagggatttt
       61 aagtcccttg tgtctgccta ttccaccaca gcggcattta gtgtttatgt ggtgattaac
      121 cttgatatat tatacactta tttttactgg ttgtcaagtc tttttttgaa attttctgaa
      181 attattttct cttttttcgt cttgtatttt gttccgaaat agtgtaaaat atttatagga
      241 atatgcccaa aaaacaggat acattttcca atcgttttac aaaaatattg aaaggggaaa
      301 taccaatgag gatagctctg tttacagaaa catacctgcc gagcatcaac ggtgtagtca
      361 cccacgttaa aactttaaag gaaggtcttg aagcgctcgg acatacggta ctggtggtta
      421 ccgctgattc aagagtaaac aatcatgtta ttgcggacga tgtaatgtat tgtcccgctg
      481 taaagctcaa gaaaatctat aattatgata tcgcacctcc aataagcaag gagcgacttg
      541 acaagataaa gagctttgca cctgatataa tccatattca taacgaattt ggtgtcggaa
      601 tatccggtgt actgatagca cggacgctca aagtgccgct tgtgtataca cttcacacca
      661 tgtatgatga ctatgtgtat tatgttgcta agactaaggg ctttggcaag atagttactt
      721 cggcgagcca tctttacgca aagatgcttg catcaacagc ttcggctatt acaggaccat
      781 cgcctaaggt ttctgaatac ttcaaagcct gcggcgttaa gaagcccgtc cacgttatac
      841 ctaactcagt ggagcttgac gtatttaagc ccgaaaacgt tgataaaaat gacgctgtcg
      901 agctgagaag aaagttcggt tttgccgatg atgatatggt tttctgtttt tgcggcagac
      961 tcggtaagga aaagaacata acgttgcttc ttgaatactg ggctaaaaat gtcagaccgg
     1021 aagacaagat aaagctgttt atacttggtg acggaccttt gcacgaacag cattgtaaag
     1081 aagctaagga gctcggtata gccgatatgg taaagtttgc aggcagagtt gaacacccgg
     1141 atttacccgt ctattatgct tgttgtgacg catatatcac cgcttcgctt tcggatacct
     1201 gttctatttc tatgcttgaa ggtatggcaa tgcatctgcc tgtcctaagc cttaaggacg
     1261 atcttaatgt cggacaggtc aaggacggtg taaacggtta taattttacc gatgcggaca
     1321 gtatgtacga gcttctcaaa aagctccgcg atatgccgaa ggacgagctt gccgagttcg
     1381 gcagacagac acgagagtcg attaaaacat caggcgcgat acgtcttgca aacgatttgc
     1441 ttgatgtata taaagaggct ctggaagtct ataagaaaaa gaacagaaaa cgcaagctca
     1501 aaaaacgcta tcgtgttatc agacacaggt atgtcagacg ctcaagaaca tataagaatt
     1561 aaaacagagt gctccgtgtt atccgatacg gagcatagct tttagaggtg tcatatgtca
     1621 cagatagata tacgtttctt ttcgcagtcg cttaaccgat atgcttcatt caaaatgtat
     1681 attcccgatg ataagcgcaa ttacggcggt gtttatgata agcagaatat gaaaacactt
     1741 ttcattctgc acggttacac acaggacggc aacaactgga taccggaata tattgcggaa
     1801 aaatataact ttgccgtagt gatccctcac ggcgagaact cattctggct tgacggcttg
     1861 tcaacggggc ataagt
//
LOCUS       DAWAOG010000439         1875 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8127_length_1875_cov_2.040110, whole genome shotgun sequence.
ACCESSION   DAWAOG010000439 DAWAOG010000000
VERSION     DAWAOG010000439.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8127_length_1875_cov_2.040110"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1167..1176
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          524 a          473 c          471 g          397 t
ORIGIN      
        1 tgactacctt gacggtgaag aagcaggtat caagagtgcc agcatactgg ttgagggcag
       61 aaatgcatac ggctatttaa agagcgaaaa cggcgtacac agactagtcc gtgtatctcc
      121 ctttgacagc tccggccgcc gtcacacaag ctttgcttct gtagaagtaa tgcctgaaat
      181 caatgaagat attgacattg agataagtga agacgagata aagacggacg tttaccgtgc
      241 aagcggcgcg ggcggtcaga aggtcaacaa gacatcctcc gctgttcgta tcacgcatat
      301 ccccacaggc atcgtgtgcg cctgccagac agagcgttca cagcatcaga acagagaata
      361 cgctatgcgt atgctgaagt caaagcttct tgagatcaag gaaagagaac acctcgacaa
      421 gatctccgat atcaagggcg aacagcttca gatcgcctgg ggcgcgcaga tacgctcgta
      481 tgtattcatg ccctacacaa tggtaaagga tcaccgcaca ggctacgaaa tgggcaacat
      541 cggcgcagtt atggacggcg accttgacgg ctttataaac gcttatttaa aggcactcag
      601 cttaggacag atataatgaa aaagaacgac atcgttgaaa ttgagataac agctatttca
      661 agcgaatgtt ccggtatagg caagaaggac ggcatggtaa tattcgtgcc gttttctgct
      721 atcggggaca agcttgaagt aaagatactg aaggtaaaca aaacctactg ctacggcaag
      781 atcgaaaagg tgattgttcc ctctcctgac agagtagctc ccgactgccc cgtttataca
      841 aagtgcggcg gctgttcgct ccgtcacata agctatgagg cacagctcag agccaaggag
      901 cagtttgtaa aggacgcttt cacccgtatt ggtgggctgt cgcccgaatt tctgcctatt
      961 atcggcaatg acacgataaa cggctatcgc aacaagcttc agctcccgat aggcacagac
     1021 aaggacggta atcttatcgc aggcttctat gcctttcact cgcacaggat cgttccgtgc
     1081 gaaaagtgcc ttttacagcc cgatattttc tcggaaataa ccgccgattt tctgaaaaca
     1141 gcgaccgagt tggatatacc cgcttannnn nnnnnnaaaa atgttccgaa aattaaaatt
     1201 ctgtcggaca ggctgcttga caaatacccg cagatagtaa gctcggtaat aaatatcaac
     1261 ggcaaggaca ctaacgtgat cttaggtgac aaagagatct gccttaccgg aaaaagctgc
     1321 attaacgata cgatgtgcgg caacagcatt gcaatatcgc ctaagtcctt ctatcaggtg
     1381 aacacacccg ccgccgagaa gctctacgcc gccgcccgcg actttgcgga agcctcaggg
     1441 aaaaccgtgc ttgacctgta ctgcggtgcg ggcacgatag ggctgtctat ggcacacacg
     1501 gcaaagcgga taatcggggt cgagattgtc cccgaagcaa ttgaaaacgc gaagcagaac
     1561 gccctcgcaa acagcatcga taactgcgaa tttatctgtg cagatgccgc cgaagccgca
     1621 gggatactgc attcacgcgg aatacgccct gacgtgataa tagtagaccc gccgcgcaag
     1681 ggctgcggta atgaagcctg cgagcagata gccgccttct ccgccccgcg tatagtaatg
     1741 gtttcctgca acgccgccac cgcagcccgt gactgcgcat attttgcgga gctcggctac
     1801 tccaccgaca agtgcgtagc cgtggatatg ttcagtggta caaaccacgt tgagacggta
     1861 gtactgcttt cccac
//
LOCUS       DAWAOG010000440         1870 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8155_length_1870_cov_1.871074, whole genome shotgun sequence.
ACCESSION   DAWAOG010000440 DAWAOG010000000
VERSION     DAWAOG010000440.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8155_length_1870_cov_1.871074"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    244..265
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          479 a          435 c          390 g          544 t
ORIGIN      
        1 acacctgtcg agcttaatca actcggcaga ggtctgtctg ccgattgtgt agtatttgaa
       61 gaagacggcg cattacctct taatgttata aattctgttc cgttaagcgg aatataataa
      121 caatatttaa agcagacatt aaagcgtact gcatatatca aaaaagctgt ggtttcgttt
      181 gtgaaaccac agcttttatt gtgtccggtt ttaagtgttc tgctttcttc tcttagcatc
      241 ttcnnnnnnn nnnnnnnnnn nnnnnttggc acgaaggtca ttgtcgagaa ttttttttct
      301 gatacggatc gtcttgggtg ttacctcgac aagctcatca tcgtttataa attcaaggct
      361 ttcttcaaga ctcattctct tgtaggggat aagtcttaat gcgtcatcac tgccgctggc
      421 tcttgtattt gtcaggtgct ttttcttgca gacgttgaca ttgatatcgc ctgccttggg
      481 agatacgccg acaaccatac cttcgtatac gggaacacct gcatcaatga acaaagtacc
      541 tctttcctga gcattaaaca gaccgtaagc catagctgta cctgactcaa acgcaacaag
      601 tgaaccgact gttctggtgg ggatctcacc catataagga cggtagctgt cgaaaacggt
      661 attcaataca ccctcacctt ttgtatctgt cataaactcg cttctgtagc cgaacagacc
      721 tcttgagggg ataaggaact caattctggt tctgctacct acaggctgca tatgaacaag
      781 ctcagctttt cttacgccca ttttttccat aacagagccg acacagttat cgggcacgtc
      841 aataagcaga cgttcgatag gttcacatct tacgccgtca atttctttga aaagtacacg
      901 aggagtactt accgaaagct catagccctc acggcgcatt gtttcaatta agatcgacag
      961 atgcatttcg cctctgccgg caacgttata agcatctgag ttttcactgt cggtaactct
     1021 gagcgaaacg tcctttaaaa gctccttgtg aagacgttct cttatctgac gagaggttac
     1081 aaattttccc tctcgaccgg caaaggggct gtcgtttacc gagaaagtca tttctactgt
     1141 aggctcgctt atctttacga aggggagagg ctcggggttg tttacagagc agatagtctg
     1201 tccgattgtg ataccctcga tacctgataa gcatacgata tcgcctatcg tagcctcagt
     1261 cacaggaacc ttgttcatac cctcaaaagt gtaaaggctt actacctttg acttgaaggg
     1321 cttgtgcgaa ccgttgtaat cgcacaccat aacttcctgg ttctgtttta ttgtacctct
     1381 ttcaattctg ccgactgcaa ttctgcctac atagtcattg tagtcgattg aagaaacgag
     1441 cagctgtaaa tcaccgtccg gatcaccctc ggggcaggga atatgctcaa ttatcttgtc
     1501 aaacagcggc ttaaaatttt ctcccataac atcgggtgag tatgaagcga caccgtttct
     1561 gccggagcag aagataacgg ggctgtcgag ctgttcatct gatgcatcaa ggtcgagcag
     1621 aagttcaaga acttcatcta ctacttcctt atttctggca tcggggcggt ctgtcttgtt
     1681 aactacaaca ataaccttaa gacccaagtc aagtgctttc tgaaggacaa atcttgtctg
     1741 aggcattgta ccctcaaagc tgtcaacaag gagcagtacg ccgtttacca tctttaatat
     1801 acgttccact tcgcctgaaa aatctgcgtg tccgggagtg tcaacgatgt tgatcttaac
     1861 accgttatag
//
LOCUS       DAWAOG010000441         1860 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8200_length_1860_cov_2.510249, whole genome shotgun sequence.
ACCESSION   DAWAOG010000441 DAWAOG010000000
VERSION     DAWAOG010000441.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8200_length_1860_cov_2.510249"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          594 a          397 c          480 g          389 t
ORIGIN      
        1 tttcagtcga tgttaccgct gagatcgacg gtttcgacgg tgataacgcc tataccttta
       61 aagtcggcaa ggttcccgag agagccgaaa agctccttga ggtcactgag gctgctttgt
      121 acgagggtat aaagatggcg gttcccggta acagaatcgg cgacatttca aatgccgtgc
      181 agacttattg tgaatcaaga ggctactatg tagtaagaaa gttcatcggt cacgggatcg
      241 gacacgatat gcacgaggac cccgaagttc ctaacttcgg caaacccggc agaggtccgc
      301 gacttgtacc tggaatggcg atctgcattg agcctatgat aaatgaaact acagccgaga
      361 tccgtatact gcctgacaaa tggacggtgg ttgaggcaaa cggtaattac agtgcgcatt
      421 ttgagcacac tatcgcaata acaaacggtg atccgcttat tctcacggaa agctcgcttc
      481 accacccgaa gaaataacgg agggcgtatg aacaatacgg ttattgcagc cggtatggtt
      541 gtaaaaagta tggcaggtca tgacagcggg agctattatg cggtcatacg ggcagaaaac
      601 ggttttgcgt atattgccga cggcaagctg cgcaaggtcg aaaagtccaa aaagaaaaat
      661 ccgctccatt tgcagaaaac attggttact gtagagcttg cggatatcac aaacaaaaaa
      721 ctaagatctg cattagcggc actgaaccgc gaggcagaca acatcgccga ggagagtgac
      781 aagcttgtct aaagaagatg taatcgaggt aagcggcaag atcgttgaag cgctgcctaa
      841 cgcaacgttt caggtcgaac ttgaaaacgg acataagata ctggctcatg taagcggcaa
      901 gctgcgaatg aactttattc gtatcttacc cggtgataag gtaacagtcg aaatgtcacc
      961 gtatgacctg actaaaggca gaattacctg gagagccaag taagtctgca taaacgaata
     1021 cgagcaaacg ctcgtctgta acaaatacag gaaggatgat attaagatga aagttagacc
     1081 ttcagtaaag cctatgtgcg ataagtgcaa ggtaatcaag cgcaagggca aggttatggt
     1141 aatctgccag gaacctaagc ataagcagag acagggttaa gccctaattt aacaggagga
     1201 aaaagatatg gcaagaattg ctggtatcga cctgccgaag gaaaagcgtg ttgagatcgg
     1261 tctcacatac gtttacggaa tcggcagaaa gacagctaac gacattctgg caaaggccgg
     1321 tgtaaacccc gacaccagag tcaaggatct taccgatgat gatgaagcta agatccgtga
     1381 tgctatagag cagctcggcg tagctgtaga aggtgacctc agaagagaag tagcgctcaa
     1441 tatcaagcgt ctggttgaaa tcaactgctt cagaggcaca cgtcaccgta agggtcttcc
     1501 cgttcgtggt cagagaacaa agaccaacgc aagaacaaga aaaggtccta agaagaccat
     1561 cgctaataag aagaagtaat ctttacggaa aggtggtaaa acaatggcaa aggcaaccgc
     1621 tactaagaag cctgtagttc gcagacgtcg tgagcgtaag aacattgaaa acggttcagc
     1681 tcatatccag tctacgttca ataatacgat agttactatc acagaccttc agggcaacac
     1741 attatcatgg gcatcagccg gtgaaatggg tttcagaggc tcaagaaagt caactccttt
     1801 cgcagctcag acagccgctg aaacagcagc taaggcagct atggagcacg gtctgaagac
//
LOCUS       DAWAOG010000442         1857 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8222_length_1857_cov_1.973363, whole genome shotgun sequence.
ACCESSION   DAWAOG010000442 DAWAOG010000000
VERSION     DAWAOG010000442.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8222_length_1857_cov_1.973363"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    941..950
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          523 a          369 c          453 g          502 t
ORIGIN      
        1 atatctgccg tcggttatat tatagtcgga ttgtatgtag cggtacagct ctttcatact
       61 ttcgtcaaaa gcaactttca cagtttccgt acagatgtaa ttgtcgcttg cgcggtaact
      121 gataatagct ttatcggcat ctgcagaata gacaacgggc aggttttctt tgaaaaatcc
      181 cggtgcatct tcgggcataa caccgtcaaa gctgtaaccg gagcgatcca tcatatttcg
      241 atataccgca ttactgctgt tcatagcgca aaacaaagat aagaatgtgt atactgctga
      301 aatcgagata agaaagataa gtatattcag cttgatatcg cttttaagaa agctgtgaag
      361 catactaaga tacaggcttt tgtatttcat atttctcctt ttttatatac cggtgcttca
      421 gaactacgtt atcggtttat aaagatagtg ctataatcta ctgtattatt tattatagca
      481 caactcttcg ggtatgtcaa tagggaaaaa ttttaagttc tgtaaagagg aggcgacgag
      541 agcgacgctg atttatgccg aaggctttcc ttttgcgttc tttgggatca ggcggtagcg
      601 tggcttttcc tcgcaaaaca ttttccagcg gagaatatca tcggtgaaaa ttccgacggc
      661 ggaaaggaaa aaccacaata cagtgaacag caggcatatc tgaccaaaga ggttgaatgg
      721 cagttgctcg tagttccata ccttccagtc gagccagagg tttactatac agccgcagat
      781 aaactcgagt gcagtgataa ttaccgatga taccgccatc tgagcgggca gtgagagatc
      841 aaatttctcg tttatgccac cgacaagcag aaagcagatc ccgcctaaga tgaacattgt
      901 ccagtgactg ttgccgcgga acagaagctc aactatagtg nnnnnnnnnn gtgtatgcgc
      961 cgcctccgaa gcagaacagc ataaacagct tcatcggcag acagattttg taattcatat
     1021 ttttaccttg cctttccatg agaatttgta cataactttc catgagaatt tgtacataac
     1081 ctttctatga gaatttgtac atattttttg cagtggacgt acaatttatg cagaagagga
     1141 gatttttgtg catataatat tccgagaaaa ggaacggagg ttttttatgt ctgctttaaa
     1201 gaaaataact gcgttttgta cagcgtttgc gatgattatc ggtataagca gtgccgcgtg
     1261 tgcggagggc acggcggaca acttgtgctc cacggctgag ggggctgtag tcactgaaat
     1321 tacaacaggg caggtgctgt atgagtataa ctcacacgaa aggttgccta tggcatcggt
     1381 cactaagctt atgtgtctgc ttatctgggc ggaggagatg gaaaagggca cattcggtat
     1441 ggatacggtt ataacggcta ccgcaagagc gaacgctatg gacggctcgg tgatatggct
     1501 taatgtcggg gagaaaatgt ctgcttacga tatgatacgc tcggtcgtta tcgcctccgc
     1561 aaacgatgct tgtgtggcgg tatgcgaaca tatagcgggc acggaagaaa tgttcgtgga
     1621 gcgaatgaac aaaagggcgc aggagctcgg aatgagcgat acgcattacg acaactgtgt
     1681 cggctttgac agtgatacgc actatacatc ggcatatgat acggctcttc tcagtgccgc
     1741 agtgtcggaa tacgactgct acaacgactt tttcaacaca aggcttgatt atgtgcgtga
     1801 gggagagcgg cagacacagc ttcttaacac aaacaagctg atgcaacgct atgacgg
//
LOCUS       DAWAOG010000443         1849 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8266_length_1849_cov_2.270346, whole genome shotgun sequence.
ACCESSION   DAWAOG010000443 DAWAOG010000000
VERSION     DAWAOG010000443.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8266_length_1849_cov_2.270346"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          469 a          467 c          374 g          539 t
ORIGIN      
        1 gcataaggta ttcgccgccg tgttcgcttg ccttgaagcg tgtaagtaat ttgttcataa
       61 aaaacctcgt tctagtcgcc tgcaagaaat ttattattgt gaagctggga gcgcagcttc
      121 acaccctgct ttttcagaat atcgaccttg agcgcaacaa gattacggtc ggtacagagt
      181 gccgccgcag ttttgtcaat atcgtagccg caatttatgc aatcgtaaag ctcttcatct
      241 gatataagaa actgtgccgc aaagagattt gcttcatact ccatagaacg atcgcacata
      301 tcgaatatgt catattcggc aaagccgccg caatcgtcgg ccttatccct gtggagcctg
      361 tcgtgaccga tctcgtgcat caggacgatt tttttcatac tgtcgcaaag atcgcgcttt
      421 ataaagatga agcgattttt caatatcagc ttatatgcac ccttctgatt tttgaagtca
      481 cgttcaagaa cctctatatc aagctctctc gcaagccgtt tcggactgca cctgccgtac
      541 agcccgataa gctcgtcagc ctttgcgacc gcttcgttaa aacaactaag cataaccgct
      601 ccttatcgct ttgcggcgta ttttctgttc ttttccttag ctatccagta agcgtcctgt
      661 atagccttca tcatctcgtc catatcatcg gggtcaagct cgccgcctgc aaacagaccc
      721 gttacctcct gcataagtgc ctttgcctgt gcggcaccgt ctgtgccgta cttttcaccc
      781 gcatttatgt aaaattcttc gttttcggtg agcagataat tgatatccac accgagtatc
      841 tcagccattt tgccgtacat ttcacgctgc tttgggtagc tgtcgccttt ttcatagttc
      901 gttatagtcc tcagcgaaac accgagcata tcggcaaact gctgttgtgt aagacctttc
      961 gcgcggcgct ctttttttac tttatcacag aatttcataa ttactccttt tacttgcagt
     1021 atgctactgc gtctgcctga aatttaagac cgttttctcc gccgatatgt gcaaattcgg
     1081 tctgtatgga tttacgaaca gggtatttgc cgccgaaacg ttcctttatt accttttcca
     1141 taaccggaat attccatata tccctgaaaa gacaatccat ctgcactacg ttttcaagcg
     1201 taagtccgac aagggcaagc cgtttttcca tttcgtcaaa tgcgccattt acctgctgtt
     1261 cgatactgcc gccgacattt ccggcgcagt aattaagaaa acagaaatcg cctgctttga
     1321 taattccgca atgcgcccat tcttcgttga catcataacg ttcaatcata atcgtatcct
     1381 ccgtaaaatt caaaaacatg caaatacgtt gcacttttct cttgacaaac gcagaatatt
     1441 tgcgtataat cttaactgga aacttaactg cgtgtttatg ataccataaa ttgcgtatct
     1501 tgtcaagacg ataattagga gtgatgatat gttccgtgat attctccact gcgatatgaa
     1561 caactttttc gcatcggtag agtgcaagct gcaccccgag cttaaaggct atcctgtcgc
     1621 ggtgtgcggc tcggtggaag agcggcacgg catagtcctt gccaaaaacg agcttgcaaa
     1681 ggcaaagggt gtaaagaccg ccgaaacagt agcatcggca aagtcaaaat gtgccgggct
     1741 tatcacagtg ccaccccact ttgaggaata ttcaaaatac agcaagcttg cacataagat
     1801 atacgagcgc tacaccgacc ttatcgagcc gttcggtatg gacgagtgc
//
LOCUS       DAWAOG010000444         1844 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8300_length_1844_cov_2.498603, whole genome shotgun sequence.
ACCESSION   DAWAOG010000444 DAWAOG010000000
VERSION     DAWAOG010000444.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8300_length_1844_cov_2.498603"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          589 a          379 c          425 g          451 t
ORIGIN      
        1 gtatcgctcc tgatatcatc gctattcata agcgcatacg acaggtcgct gtccgaataa
       61 ttgaacagcg catagccttt gccggacgcg ttgataagca tagctcctct tgctttgttt
      121 ttcgctagga tatccgcaat atcactgtag gaaagagcgt tatcgctgta gttatcctga
      181 gtgatctcaa gcaatacggc atcgtccgaa aaacggtcgg tgatatccga agagctgata
      241 ttgaagaata atgacataag aatggtgcag agtgtgagca cggcagagta aaatactgtt
      301 atcatgccgc ttttgttgaa cagttgccgc atttattgtc ctttcgcagt gggattgata
      361 ttgattacat aatatcacac acagaaagat atgtcaagaa atccatattt gtattgaaag
      421 caaagcctgt atatgctaaa atatagttat catattacag gaggagcatt tatgcctaca
      481 gaactcgaca caatgaagca tgcaaaggag tacatagaca agcttgcaaa cgggattgat
      541 ccgtttaccg acaagcctgt acccgatgac gacattgtaa ataacgtgaa gctgtcgcgc
      601 tgttttttct atatttcggg gatacttgaa atggcggaga agtatcgcct gttaaaaccg
      661 tcgaagtcgt aaaatcggga agacgcggtg tggtggttat caccgatgag gcgagagccg
      721 ctttcaagtg ctttataaac gaagttacgc cgacagcgtt taccgatatg ataaacaagc
      781 accttgaccc ggatatatca ggcaggctca agaaaaacgc tattgccgac tggctttctg
      841 aggtcggtat acttgaaaac tatgagcttc cgaacggtaa aatgcgcaga cgacctacag
      901 aaagcggaag caatgaaggt atacgtctga gtgaatattt cagcgcggac ggttttccga
      961 gaacggcgat tatgttgagt cctgcggcac agcagtttat ccttgacaat atagacgcta
     1021 tagcagaaat gaacggagcg ccgagagaaa gacgtaaaag aaaaccgttt gatgtaacaa
     1081 acggtatgag agataattta tcggctaagg aagtacctat gtctgcatcg gcgatcacag
     1141 ccgaaattaa ccaatatgta gatgccgaaa attcgggcac actgaaagcc ggaatagttg
     1201 caaaatggct tgaaaaacag ggaatacttg aaaatacgga actcccgggt ggcagaaaac
     1261 gccgactgcc aaccgacgaa ggtttacagc ttggcatatt caccgagcag agaacatcaa
     1321 tgagcggaga tgaatataca gctgtgcttt atccgccggc ggcacaagag tatatttaca
     1381 gccatattga tgaaattgcc gattttaata acggataaca taaaaccgga gcaaaaaaac
     1441 tccggtttta cttttatcct ctgacattat aatcctgtgc aagaagcaca gacgcttttg
     1501 cgagcttgtg cagataaaca aacggtccga caatgataag acttcccaga acgttccaga
     1561 gccagtaatc cgtagcaccg aaagaatatg ctatgccacg tcttgcaagc tcgttgccca
     1621 ttctcgaaga aaggttatga aaccatgcga taaagctgaa accgaatgta agcggtccga
     1681 caataaacgc cataaggcag taatgcattg tcttttttcc gtcaaatctg cttgcgataa
     1741 gatttatatc ttctgaaatc ccggaaaaga aaactaacgg ataaattccg aaagttacaa
     1801 tcgttaacaa aatcagctta agcagacctc tgcgcgtatt gagc
//
LOCUS       DAWAOG010000445         1842 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8315_length_1842_cov_2.339675, whole genome shotgun sequence.
ACCESSION   DAWAOG010000445 DAWAOG010000000
VERSION     DAWAOG010000445.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8315_length_1842_cov_2.339675"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          430 a          436 c          445 g          531 t
ORIGIN      
        1 ggtcaagagc aacgtaatcc ctgttcttga tgagcgttct gtaggcagga cgcatgatac
       61 gcttgccgtt ttctatttct tccattctgt gagcgcacca acccggcatt cttgcacaag
      121 caaataaagc ggtgtacatt tcgaccggta tgccgagaag cttgtaaaca agtcccgagt
      181 acatatcaac gttagcgcac attgtcttta tatcgccctt gttcttcgca aacagctgag
      241 gagtaagacg ctcgatagta tcgagaagtc tgaactcggc ttctgtttca gtgccctttg
      301 caagcttcag agcagctttc ttgagctgta cagctctggg gtcagaaagt gtgtagactg
      361 cgtgtcccat accgtagata agaccgctgt ggtcgttttc ttccttgtta agtatttttg
      421 tcaggaagtc tgcgacctcg ccttcgttct cccagttctt aacgccgttt tcgatgcagt
      481 taagcatctc cataactttt atgttcgcac cgccgtgacg gggtccttta agcgccgata
      541 tagccgaagc gtaagccgca taagcgtctg tgccggtcga ggttacacag cggcaggtaa
      601 atgtcgagtt gttaccgccg ccatgctctg cgtggagcat aagacaaagg tcgaggagct
      661 ttgcctcctc ctttgtgtac tcacggtcgt cacgcagggt ggacagaatg ctctccgcaa
      721 ggctttcgtc aaggtttatc gggtgcatta ccatgcttga gttatacaga tagcggcgca
      781 ttacctgata agcgcctacc attattgcag gcagtcttgc aattaccgag atcgccttga
      841 gcatttcgct ttccatgctc ttgttctcag gttctttgtc atatgtgtag agtgccagca
      901 ccgactgtgc catcttgttc attatgttgg atgacgggca acgcataagc acgtcctccg
      961 caaaataggg gggcagagtg cggtagtgtg ttactatctt cttgaagtcc tcgagatgct
     1021 ccttgtcggg caggtttccg aaaagaagaa gataagccgt ttcttcaaag ccgtatcttc
     1081 cgtccttttc cacgccgctt acaaggtcac atatatcata acccctgtat atcagctgac
     1141 cgggcgcggg acgcttttcg ccctcgtcga taacgtagcc gtgtacacag catatatttg
     1201 taagacctac aacaacgccg cttccgtcat tgttacgaag tcctctcttt acattgtatt
     1261 tgtcatataa tctcgggtct attgccgtct ggatcttgta atcgcggcat agcttctgaa
     1321 atcgctcgtt catacatttt attcctttct gtctttgtac gtttataata gcctcgcata
     1381 ttctatcccg taaaaacggt gaatataatg aattaaggac gaacaggttt gccgaagcgt
     1441 ctattcttta ttattatact ctattttgtt tgataagtca agaaaggaac gtatatcgcc
     1501 gtgaaaaaga aatatctgta tcttcttata gcaggactgg cgctgtcagc gcttttacct
     1561 gtggttatac ttatgtgcct gtgcacaagg tcagagcaga tccgaccgca gacggtgatg
     1621 tatggcggaa ttgaatatac ctataaggag tttcttaccg ggtgcatatt gagcagcctg
     1681 aaaatgcttg acgaaccgcc agatgatgac gatgccgagg gaataaaagc cgtggcttct
     1741 gcgcttgact gttcggcaag atatctgctg agggcgggaa agactcttga cggcggatat
     1801 ccgagtcttg agcacacgga cgaaagggaa gggctttcac at
//
LOCUS       DAWAOG010000446         1837 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8356_length_1837_cov_1.901235, whole genome shotgun sequence.
ACCESSION   DAWAOG010000446 DAWAOG010000000
VERSION     DAWAOG010000446.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8356_length_1837_cov_1.901235"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1606..1615
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          517 a          388 c          358 g          564 t
ORIGIN      
        1 gttacaagaa cagcttcaat agcgtcaact atagatatct ttctgccgat aatgttgtca
       61 ttcttaacat aaacgatcat tgtagccgct acctgactga gttcatcctc tgtagcatcc
      121 tttgtcgaat cggtcataag agtcttgata tactggaagg aagccttatc gataccaaac
      181 tgtgaaagga tatccataat cttgctttcc ttctctaccg catcaagtac atctgtacag
      241 aaagcgatca ccttttcaac tgtaaaatca attgtgtacg atgtgcaatc tacggtactg
      301 tccgacattt tgaatgaatc agacttgttt tcaacgcttg taaagttgtc gaaataagct
      361 tcggtaataa tgtcaacgat cttgtcaaat gtttcgtcat ctatattctt cagtatctct
      421 tcataatttg tcgatgccga aggagcttcc gctacatctg aaacggtaag aatgccgctg
      481 aaatcagagc ttccgccgga ggaaatatcc tttaaagatg aggaaagctc attgagatcc
      541 attactatgt acttatctgt aagctcgggt acagtgtagt acattttgcc gttggcaagc
      601 cagaagttta cattcatata agtttcatca cccgcaccta aagcaagtga tcccgatccc
      661 ttttcagacg cgctgtcaac gttgctctta gcctttataa ccgtaggagc aatatcggta
      721 tcagaaccta tcagatcttt tgatacattt aaggtaatag tctgttcgag accggtcttg
      781 acattcttgg cactgtttaa taattcatcg gctttctttt cggcagatgc aaaataattt
      841 ctttctaacg cttcataacc gcttgccttg ttcttgaaca gattaaggca ggcaaaaact
      901 acaccgcctg caacaattgc tacagcaagg ataataagaa tcatcggcaa aaccttgctc
      961 tttttctgag gaggtacaac tgcgaaatta ccggcatcat atgcaggcat accctgagga
     1021 gtaaactgtc cctgagcctg tgcctgctgt gcaaactgct gaggctgtgc ctgctgtgcg
     1081 aactgctggg gctgtgcctg ctgtgcgaac tgctggggct gtgcctgctg tgcgaactgc
     1141 tgaggctgcg cctgctgttc aggttggtca ctaaattcgt tgagcgcctg ctgccagttg
     1201 ggctgctgat tgttcgtgtt gttattttcc atgatatgtc ctttcgagat gtggatttaa
     1261 atgatatatt tcctatataa tctgacacgg tgacccgtat catccagtta cattttacca
     1321 taacagctga aaaaaagcaa gtcggtaata taaaaaatat atggctgtat ccgtttttca
     1381 ggtgatatag gaatatataa cggagctgat gcgattgatt tgttgaatgt aaacaaagaa
     1441 aacgctatat acaaacagca aaaaactgtt ttgttaaagc attgaaaatc atctatttcg
     1501 ctcctcttcg gcacatataa tcaatttttg ttttcggaat attccgttca atttaccgaa
     1561 gtaataaacg ctgtaaatat gcgcttcaac agcactgtag tcaagnnnnn nnnnntttct
     1621 acaaaatctt aagttttgcc gaattttgat gtcaggtttt tgcgttttga acgtttatat
     1681 atatgagggt gttttttcag agttcatata actatcctga tatcatcggt aaaaatttaa
     1741 aatgacccca tcataaaaac tacgccgatg acaatgacaa tgctacgcta atccttacgc
     1801 aaaacacctg cgaccgccgc acaggcatta ccaaacc
//
LOCUS       DAWAOG010000447         1837 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8357_length_1837_cov_1.716611, whole genome shotgun sequence.
ACCESSION   DAWAOG010000447 DAWAOG010000000
VERSION     DAWAOG010000447.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8357_length_1837_cov_1.716611"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    821..830
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1281..1290
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          547 a          329 c          492 g          449 t
ORIGIN      
        1 actatgtgtg gagcaatatt tacggcataa ccgtaggacg ttttcaggat ttctatgtat
       61 cgaagcacgg aaacgagttt gtgtttcctg caggtaaggg cgacacagac gcactgctgt
      121 ccgagcttaa aaactactgc gacagtacag gtatacagtt taaattctgt agtatggata
      181 taaaggataa gcatatgctt gaagagcgtt atcccggtat gttcacattt gatacaacag
      241 aggacttgta cgactatgtt tatgagtcaa agtcacttgc aacgctcagc ggaaagaagc
      301 ttcactcaaa acgcaattat ataaaccgtt ttaaatctct ggactggagc tttgaagata
      361 tcactccgga aaacatagac gaatgcagac ggatgagtct tgaatggtgc aggcgcaaca
      421 attgtgatga gaacgaggaa aaatccgagg agatgtgtgc ggtcggaatg gggttggcac
      481 atttcttcga gctgggtctt gtcggcggta tcttaagagt tgatggtaag atacaggcgt
      541 tctcctacgg tgaacgtcag aacagtgata cgtttgtgac tcacgttgaa aaagcgttca
      601 ccgactatga cggaacttac ccgatgataa actatatgat ggcaaacagg ttttgtaaag
      661 agcttgaata tataaacagg gaagaggata tgggcgaaga aaacctccgc aaggcgaaaa
      721 ggtcatacta cccatgcttt atggtagaga agttcagtgc agtctataac gctgagaaag
      781 gataacaata tgatatatgt atttttagca gacggatttg nnnnnnnnnn tcggagtagg
      841 cggcggtaag gtgacaggcg cacatggaat taccgttaca gcagatatta ccgacaatga
      901 cgttattatg accgataagg tcgagatgat agtgctcccg ggaggtatgc cgggcacact
      961 taatcttgaa aagagccctg ttgtccaggc ggctataaag ttctgtgccg acaataaaaa
     1021 gtcgataggc gctatatgtg ccgctccttc gataatcggc aggctcggac tgctcagcgg
     1081 taagaaggct acctgcttcc ccggatttga acagttcctt gagggcgcta tgttcacggg
     1141 cgatcttgtg aatattgacg gtgatatcgt taccgcaagg ggtgcgggcg cggctataga
     1201 atttgggctt gcactgggcg cgttgctttg cggagagaaa aaggcagaaa agatagctaa
     1261 ggctttacag tgcagataac nnnnnnnnnn aagtgaaaca gctgttaaaa attccgctat
     1321 aaccgagaag cttttaagcc ttgcaaaggt gcagtcggca gagcttatat tgccgtttgt
     1381 ttcggcaaag ggcgaggtcg gcacaagaga gtttatagcg agatgctttg acgcaggaaa
     1441 gactgtcgcc gtaccgagat gtattgatgg cagtaatatg aagttttgtg ttatacatac
     1501 gtttgacgac cttgaaaagg gtatgtatgg aattgacgag ccgaaagagt actgtgaggt
     1561 aataaaagcc gagaacgcgg aaaacagcgt tctgatagtt cccgcattat gctttgacac
     1621 taagggctac agactgggct acggaaaagg ctattacgac agatttatca gcagatacag
     1681 cggctttaca gtcggagtat gctacagcga gtttataacc gatgatatac cgatcgatga
     1741 gtacgacaga tgtgttgata tcgtcattac agaaaagcag aaaggaaaga aagatggaga
     1801 ataatccttt agatacggtc ggaggcaatg gaaacat
//
LOCUS       DAWAOG010000448         1835 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8366_length_1835_cov_2.443820, whole genome shotgun sequence.
ACCESSION   DAWAOG010000448 DAWAOG010000000
VERSION     DAWAOG010000448.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8366_length_1835_cov_2.443820"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          476 a          479 c          382 g          498 t
ORIGIN      
        1 attatagaaa gcgccatcac ctatactcgt cacaccgtcc ggtatcgtta tgcttgtaag
       61 gcttttacaa ccgtcgaaag catatttgcc tatctctgtc acactgtccg gtatcgctat
      121 accggtaagg cttgagcaac cgctgaaagc tctccggccc atctccgtca cactgttcgg
      181 tatcgttata cttttaagat ttgtgcaacc ttcaaaagca cgcctgccta tactcgtcac
      241 acttttcccg tcaattgtat cagggataac caccgtttcc gcaccgccgt tataacctgt
      301 gatcttgacc ctaccgtcat caagcgcgct gtactcaaaa tcctgatacg tttccgctcc
      361 cgcacataca gcgcatacca ccgtcataac agcgaaagtc gccagtgcca atagccactt
      421 tttaagtttc atattaccat ctccttaaag ctaactctga aatgcaaaac agcacagccc
      481 gccgcgagct gtgcctgata tcaaaatatg tataccgtct tgatatcaat gcatatctcc
      541 cacgggtaat aagctgtatc tttatgctta actgtgtcaa gtatatcata ataccatatg
      601 cttgtcaagg cgcattgatt aagtattgtt atcaccctca ctccgcacta tccccacacc
      661 ccgcgccgcc acaacttgtc gcccctcaaa aaagagaaga tatcacggcg cattataact
      721 tttccgtaat tcgtttatcg agtagatgct gtttcttagg caacagaaaa cccccgggat
      781 ttacactccc gggggcttaa ctatcctata ataactttcg ttttaccgtc tttacagatc
      841 aatacttagc gtaaggattt tcaccgtcat aaagcatacc tgcgggacgg ttgaattcaa
      901 gatcctcaac accgagatac ttcagtgtat cgaagatggc cttgccgtag tgaccgaatg
      961 caaccgcacc gtggtgagga tagttcttct tgataagaac attgcggtag aatctgccca
     1021 tttcgtggat agcgaatacg ccgatagcac cgaatgaacg agtagcaaca gggagaacct
     1081 caccctcggc aacataagct ctcagcttag cgtctgctgt gctctgtaaa cggaagaatg
     1141 tgatatcgcc gggaacgatg tcgccctcaa gtgtacctct tgtgatatcg ggctccttat
     1201 caggctcaag acctctgtgc atgatgagct gatacttcat tgtcttgctt gtcagcttgc
     1261 atgaagcagt gttaccgcag tggaagccca tgaatgtatc cttaagtgtg tagttgaact
     1321 tatccttgat agactcaaca tacatatctg cgggaacaga gttgttgata tcgagcagtg
     1381 taactacgtc ttccgaaacg caagtaccga tatattcgct gatagcaccg tatatatcaa
     1441 cctcacacgc aacagggatt cctcttgctg taagtcttga gtttacatag cagggaacac
     1501 agccgaactg tgtctggaat gagggccagc acttgttagc aaatacaacg tactttctgc
     1561 ttcccttgtg tgcttccatc cagtcaagca gtgtgatctc gagctgtgca agtctgggca
     1621 ggataccggg catcttgttg ccgtcgccaa gctccttttc catatctgca acaacttcgg
     1681 ggattcttgc gtcattctta tgctcgttga atgcggcata aaggtcaagc tccgagttct
     1741 cttcgatctc aacgccaagg ttgtacagct gctttatagg agcgttacaa gcgaggaagt
     1801 cctgaggacg aggaccgaat gagataacct tcaag
//
LOCUS       DAWAOG010000449         1825 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8435_length_1825_cov_1.827684, whole genome shotgun sequence.
ACCESSION   DAWAOG010000449 DAWAOG010000000
VERSION     DAWAOG010000449.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8435_length_1825_cov_1.827684"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    411..420
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          467 a          406 c          387 g          555 t
ORIGIN      
        1 gccttatggc gcttgccggt gacctcggct gtgcatcagg accgttcatt gccggtatga
       61 tctgcaactt tgccaccgca aacggtgcaa atgaatccgc aggcatgaaa acaggacttg
      121 gattttccgt attgttcccg gtaattctta tagtctgtac gcttattttt acttttgtta
      181 tcaaaaaacg taagtcgatt taacttctcc gtaatttttc tttacaaacc ccataaaata
      241 tgttaaaata tgattttgga atactatttt aatataaaca gattttacag gaaaattgcg
      301 aatgaaaatt gaccagataa aaatgggctc actgctctcc tatctatcgc tgatactcgg
      361 tacggtcgtt tcacttgtct acacacctat aatgatagaa aggctgggac nnnnnnnnnn
      421 cgtatactcc atagttttgc ctatggtatc atacctgaac cttttcagct tcggtcttgg
      481 cagtgcctat acaagatttt acaccaggta caaagaagcc ggtgacaagt ataatatggc
      541 taagctgaac ggtatgttcc ttatcatata ttcgataatc ggtattgtcg ttctgatagt
      601 cggttttact attgctgcaa atccccgcct tgcattcgga cagaagctta ccgatgcgga
      661 gatagacctt gccgtaaagc ttatgtacat tatgactgtc acggcggcgg ttagttttcc
      721 tctgagcgta tttgaatcga acactatggt aaacgagcgg tacatctttc ttaaactgct
      781 tgccatagta aagtcggtaa taaatcccct gcttataatc cctctcctga tgataggact
      841 gcgctccgag gcaatagcat atatgagcct tgcgttcacg ctcttctcgg gtgtactgaa
      901 tatcatctac tgcaacaaaa agcttgctat ccgattttat ttccgtgact tcgatttcaa
      961 gcttctgaaa tcgatgttca agttcacggg ctgggtattt atcggaattg tggtcgatca
     1021 gctaaactgg agcgttgata agttcctgct tgcgtggatg cacggagcaa acgaggtagc
     1081 gacatacaac gttgcatctc agctcaacat ctactatatg tcaatggcga ctacattctc
     1141 gggaatactc acaccgagag ttcatcagat ggtcgcaaac agagttccta acaagcagat
     1201 aagcgacctg tttatccgtg tcggacgttt tcagttcatc atattgacaa tgatactgct
     1261 cggatttgta gcggtaggct atcctttcgt tctgctgtgg gcgggtgaag ccaacgccaa
     1321 cgcgtttgtt atcgctcttc tgctgttcat acctaccatt cttccgagta tacaaaacct
     1381 cggtattgaa atacagcgag caaagaatat gcacaagttc cgttctcttg tatatgtgct
     1441 tgttgcgata ctcaacataa taatcagcat accgctgtgt gccctgttcg gctctgtcgg
     1501 tgtcgcaata ggtacagcga tccccgtatt tatcggcaac ggacttataa tgaactggta
     1561 ctacaataaa tatattgggc ttgatatccc gaggttctgg cggagaatta ccgcactgct
     1621 cccatctctt gttgttcccg ccattgcggc gatacttata gcgtgtctgg ctcacgtttc
     1681 gggctatttc ggaatactgc tctggggcgg catttatgtt gtgatattca taatatcggt
     1741 atggattctc ggacttaccc ccagagaaaa agagcttgtt tccactccgc tcaagcgtat
     1801 gatatataaa atagtgtcaa agcta
//
LOCUS       DAWAOG010000450         1825 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8433_length_1825_cov_1.944633, whole genome shotgun sequence.
ACCESSION   DAWAOG010000450 DAWAOG010000000
VERSION     DAWAOG010000450.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8433_length_1825_cov_1.944633"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          416 a          468 c          385 g          556 t
ORIGIN      
        1 tccgacagct ttgcaggcgg catatcatgc tgatactgac ccacgccaat tgccttgggg
       61 tcgatcttca caagctctgc aagcggatcc tgaagccgtc ttgcaatcga tactgcactt
      121 ctcagtgaaa catcatactc gggaaattcc tccgccgcaa gcttacttgc ggaatatacg
      181 cttgcgcctg cttcggatac gaccatatag cttaccttgc ggtcgatagt tttcagcaga
      241 tcggctatga acacttcgct ctcgtggctt gcagtgccgt ttccgatagc aataatatcg
      301 accttgtgct tttctataag cgattttatt atcttaccag cacccgcgat gtcgctcttt
      361 ggcggtgtgg gatatacaac tgatgtatca agcacctttc ctgtagcgtc tactactgcg
      421 accttacagc ctgtccggta gccgggatca aaacccagcg caacgctatc cttgacaggc
      481 ggctgcataa gaagctgtct taaattatcg gagaaagtat gtatactgct gtcgcaggct
      541 ttctctgtaa gctcgtttct gagccttctt tcaagtgaag ggtgaataag gcgcttgaaa
      601 ctgtcgtcac acgcagaacg taccagtctg ccgcaatcgt tttccgcctt gacatacgct
      661 ctgtagatat gcttcataac ccactgctcg tccggctgta cagtaacctt taacgcctct
      721 tcacgctcac ctctgtcgat ggcaagaagt ctgtgcggcg gtatcctgca cgcaagctcg
      781 ctgtagtcat agtaattatt gtagaccgtt tcagcttttt catcggttgc tttggacacg
      841 atgagtgcat gacccgtgta ttcggcatac agctttttgc gaaggtcggc atcgtcagaa
      901 actgtttccg agattatgtc aagagcgccg tttatagcat catctacagt ttcaacgcct
      961 ttttcttcat ttatatattt ctgtgcttcc gcagtaggag aagcaagcgt ctgggcaagt
     1021 atgaaatccg caagcggctg taaacctttt tcttttgcta taccagctct cgtcttacgc
     1081 ttgggcttga acggtctgta gatatcttct acctccgcaa gtgtctttgc gttcaaaaga
     1141 tcctgtgtta ttttgtcggt aaggtttccg ccgttttcga taagattttt aacttcttcc
     1201 tttctgcttt caagattacg caaatatgta agccgttcgc tgagctctct taaaagctga
     1261 tcgtcaagtc caccggtcat ttcttttctg tatcgtgaaa taaacggtat ggtattaccc
     1321 tcatcaataa gcttgacggt gttttcgacc tgcgtatttc ttattttaaa ttcttccgct
     1381 aactgtttta agatatccat tgcttctctc cgtttatcag aataattttc ccactatctc
     1441 aaacacagga ccgtgctgcc acaagaaggt gcttagttgt atttcaaatc cctcaccctg
     1501 atcgcaagca aagtcgatag gcttcaactt gggcagaccg tatcctgaaa gcaggttcat
     1561 aataactccc gagtgagtga taactgccgc cgaagtgacc tcgtttgcca tcatatcctt
     1621 gaatatttct tcaagaccgt caaggcatct taatgtaaaa tgtccgaagc tttcgccgtt
     1681 cggcggcgct gcatcatatc cgcctttgag ccattcaagg tacttctcgt cactttgaag
     1741 ctcagcctgc gtcttaccct cgtagtcgcc gaaatctatc tccgatattc cgtcaatgcg
     1801 cttcaaaagg cgctcgggat aaatg
//
LOCUS       DAWAOG010000451         1822 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8449_length_1822_cov_2.142615, whole genome shotgun sequence.
ACCESSION   DAWAOG010000451 DAWAOG010000000
VERSION     DAWAOG010000451.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8449_length_1822_cov_2.142615"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          540 a          391 c          421 g          470 t
ORIGIN      
        1 gcatatgtca cccgtgcctt atgctgatat cgtaacaacg accacacata agactttaag
       61 aggtcctcgc ggcggactta ttcttacaaa cgacgaggag ctttcaaaga agataaacaa
      121 ggctatattc cccggaacac agggcggtcc tttaatgcac gttatcgccg caaaggcagt
      181 ttgcttcgga gaggcattga agccggaatt tacggaatat gcaaagcaga tagttaagaa
      241 tgctgcagtt ctcgccgatt cactgcttga aaagggattt gatctggtgt ccggcggtac
      301 tgacaaccac cttatgcttg ttgacctcca gcctttcaac atcaccggca aagagcttga
      361 aaagaagctc gatgaggtgt atatcacagt taataagaat gctgttccga acgaccctca
      421 gagtccgttt gtaacaagcg gcgtacgtat aggaacacct gctgttacaa caagaggtct
      481 taaggaagac gatatgaagc agatcgcaca gtgcatctat cttactgcaa ccgactttga
      541 caacagcgca gacaaggtaa gagaaaccgt aacggaaatt tgcagaaagt acccgctgta
      601 cgaataaatt atatttaatc gaaaacgaga gatgaaagct gtccctcgtt ttcgtgctgt
      661 ataaaatgtt gaagacagaa aggaatttat aaatgacagc atcagcaaga gacattattt
      721 ttaatcattc cgaaagagta gctcctctcg gacttattgc gatcccgggt tctgaggagc
      781 tcggcaggaa ggtaaatgag taccttgtaa catggtcaca ggaagccggc tatgattatg
      841 ataatttcct tattgagaat gagtgcccca ggttttcttc cggtgacggc aaggctctta
      901 tcaagaacac cggctcaccg tatcagcata atcacgccta tcctttacgg cggaagacag
      961 cacagaagga attacagaga gtcgcttgac tgtgctgtag cgcttcagga gcttcagaat
     1021 atgggagttt ccaatatcat tgcgtttgac gctcacgacc ccagagtatg caacgcagta
     1081 ccgcttatgg gctttgataa cattatcccc acatatcagg tattgaaggc tctgttcaag
     1141 tatgtcaagg acttcaagat ggataaggat cactttatgg ttgtcagccc tgatgagggt
     1201 gcactcaaca gaaatatgta ttattcgtct gttcttggtg tggaccttgg tatgttctat
     1261 aaaagacgtg actattcgca ggtggtgaac ggacgcaacc ctatcgtagc tcacgaatat
     1321 ctcggtaact ctgttgaggg taaagatgta ttcatcgccg atgatatcat ttcatccggt
     1381 gaatctatgc tcgatatagc tatcgaactc aaaaagcgaa atgctaacta tgtgttcggt
     1441 acaaaccttt cctacagaac tcccgaactt ctcagcagag aatggttctg cgaggttgac
     1501 gtatcgaagt atctgtcata tctgattatg gctcttaacc acgatatgtc gatcagcaag
     1561 gttatagatc ctcaccagaa gataagcgct ttacttgaaa agcacatgaa cgactgataa
     1621 ggtaatatgg ttatttcagc ctctgtgacg ggcggaggct tatattttac aggagataaa
     1681 ctatgccgtt agcagacaga atacgtccgt cgacacttaa cgaggttgtc ggacaaaggc
     1741 acatcttagc gcccggaaaa ccactctata atatcatcga cagaggtaaa ataccgaata
     1801 tgatatttta cggaccttcg gg
//
LOCUS       DAWAOG010000452         1820 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8463_length_1820_cov_2.170538, whole genome shotgun sequence.
ACCESSION   DAWAOG010000452 DAWAOG010000000
VERSION     DAWAOG010000452.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8463_length_1820_cov_2.170538"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    479..488
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1411..1420
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          420 a          435 c          413 g          532 t
ORIGIN      
        1 tcgataggct gctcgtcacc gagaattttc tttctgaact cgggatcaat aggagcaggt
       61 gtcttaccat agctgccctt gacaagtccc ttgaattcct tagtaaccgt cttgtaacgc
      121 tcgccgagaa ttacattgaa tacagcctgt gtaccaacga tctgtgatgt aggcgtaacc
      181 agaggcggga agcctgcgtc tgctcttaca cgcggaactt ccttaagaac ttcctcaaac
      241 ttgtcagact tacctgcctg cttaagctga gagaggaggt ttgaaagcat accgccggga
      301 acctgatata taagagcgtt tgcgtcaact gcgaggagct tgggatcaag cagaccgctg
      361 tcgatgtact tcttgcgcag tgtcatgaag taatctctta tctctgtgag gagctttaag
      421 tcaagtcctg tgtcatattc tgtaccctgt aatgcggcaa ccattgactc tgtgggagnn
      481 nnnnnnnncc ctttaacaga cacatagatg caagacctga tgtgtagtgt gtatggagct
      541 ggatcgggat atttacagca cccttgagcg cctttacaag tctttctgtt tcatagggag
      601 taagcagagc agccatatcc ttgatacaga tagagtcagc gcctgcttct tctattctct
      661 ttgcgtaatc aatgtagtac tgttctgtaa atacttcacc gagtgtgtaa gagatagcta
      721 tctgagcgtg tgcgccctcc ttcttggcag ccttaacagc tgtttcaagg tttctgatat
      781 cgttgagcgc atcgaaaatt ctgattatat caataccgtt tgctacagac ttctgaacaa
      841 agtattcgag tacatcgtca gcatagtgac ggtaaccgag catattctgt cctctgaaca
      901 gcatctgtaa ctttgtgtga ggcatttcac gtcttaatat acgcagtctg tcccaagggt
      961 cttcatttaa gaaacgtaag catgcgtcaa atgtagcacc gccccagcat tcaaccgaat
     1021 gatagcctac cttgtccatt gtggaaagga tagggagcat ttcttcagta gtcattctgg
     1081 ttgcgataag cgactgatga gcgtcacgca gtacggtttc ggtaattcct actttagcca
     1141 taaccttatt tattcctttc agtcaagagt tacgcattga tcgttgcgat cagtgtgcct
     1201 gtttcaacag actgaccctt tgtaacattg atgcttgcga ttgtaccgtc acagcttgct
     1261 acaacgtcgt tttccatctt catagcttcg atgataacta ctgcgtcacc cttcttaaca
     1321 gcctgtccaa cattagcttc gatcttgaat accttaccag ctgcgcctgc ttctaccttt
     1381 acactgccag cgcctgctgc tgccttaggt nnnnnnnnnn tgtaccgtta gtaaccttag
     1441 cgccgactgc aagcttaacg tcaacgatcg taccgggcat gggagcgttt atcttaacgc
     1501 cgccctgtgc gcctgcggga gcagcgggag ctgctgcctt cttgggagcg ggagctgctg
     1561 cgggtgcggc aacgggtgct gcactgccgt ctgtttcttc aacggttaca tcataagctg
     1621 taccgtttac tgtgatattg tatcttttca taacaaaatt cctttcttat gtcgattagt
     1681 atacgaaatt aacgtgcttc tttgcaggag caacaactct ctttgaagat gttgcttcga
     1741 ttgcagatgt cagagccgac tttgtatcgt ctgccgtgat gaccctgtca acatatccgt
     1801 tagccgcagc tgtgaatgcg
//
LOCUS       DAWAOG010000453         1819 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8471_length_1819_cov_2.552154, whole genome shotgun sequence.
ACCESSION   DAWAOG010000453 DAWAOG010000000
VERSION     DAWAOG010000453.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8471_length_1819_cov_2.552154"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          493 a          386 c          445 g          495 t
ORIGIN      
        1 cattatagct atcggcagtg ccatagcttt tggcggcgca ggcgctatat tctggatgtg
       61 gatatctgct ctgcttggta tggcgacaag ctttgccgag aactacctcg gcgcaaaata
      121 cagaaccgta cagcctgacg gtcttacagg gggagtgcct tttgcgtaca taaaaaaagc
      181 attcggcgga aaaaagctcg gtactttctt cgcgggaatg tttgcagtat gttgcatagg
      241 cgcaagcttt ggcattggaa atatggcaca ggtaaactca gcatcaatgg cacttcggga
      301 ttgtctgagc gttccgctat gggtttctgg cattctgtgt gcaggcgtta tcgccgcgat
      361 agctcccgga ggaatccacc gtcttagcaa agtcacatcg tgggttatcc cgatagccgc
      421 aggcggttat ctgcttggtt gtcttgctgt tataataatg aacattgaga atgttccatc
      481 ggtatttgca cgtattttca cagaagcgtt ttcattcaaa gcagtaggcg gaggtactgt
      541 cggcgtggta atgctcgaag cgatgaaatg gggcgtaaaa cgaggcgtat tctcaaatga
      601 ggcaggtctt ggctcatctg ttatgatcaa cgccaactcc agtgccgatc agcatcatac
      661 acagggtatg tgggctatgc tctctgtatt tatcgacacg atcatcatct gcactatgac
      721 agcactctgc attctttcaa gcggcgcgga tcagaaatca agcgtaggct ttgatatggc
      781 tctgatagca ttccaggacg gactcggtga ctttgccggc gcattcctca gcattgcaac
      841 gtttatcttt gcgataacaa ccgttacagg ctggtggata tacggcagaa gctgctttgt
      901 ttaccttttt acaaagaagt gggtaggagt atatcttgca attttcatcg taactgcttt
      961 cgtgggcgcg gtaattcagg ctacggtcgt ctgggatgta tccgatctgt tcaacggact
     1021 gatggcgata cctaaccttc ttgctattat cctgctccgc aaggaagtta aagctgacat
     1081 ttaattcctt tataatcagt agatattcat aaattaaaat aaagcacaaa gtgaacgtgt
     1141 atcattctga tttgttttat acagccaatt tcgggttctt gcccggctgt aaatcaaggt
     1201 cggttatgta acgcttactt tgtgcaattt ttttaccgtt tttgcaattt cactattgac
     1261 ctattgcaaa aaatagtata taatagtaaa aggggaacaa ggtacgatag aactgtatcg
     1321 ataattccct accgaatgac tttcatcagc gcaagctgat aaaataaaga aaaggagaac
     1381 aatcggttat gacaaaaaat cagaatgttt taaagtgggt cgaagaaatg aaagccctca
     1441 cacagcccga caaggttgta tggatcgatg gcagcgagga acagctcaag gctcttcgcg
     1501 acgaggctat cagaacaggt gaaatgattg agctcaacca ggagaagctc cccggctgct
     1561 tatatcacag aacagccgaa aacgacgttg cccgtgtaga agacagaaca ttcatctgct
     1621 cgagagagaa ggagaatgca ggtcctacaa acaactggat ggatcctaag gaaatgtacg
     1681 ctaagctgac aaagctctat gacggcgtta tgaagggtcg tacaatgtac gttatcccct
     1741 actcaatggg acctatcggt tcacccatcg ctaaggttgg tgttgagctg acagactcta
     1801 tctacgttgt tctcgacat
//
LOCUS       DAWAOG010000454         1816 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8498_length_1816_cov_1.939807, whole genome shotgun sequence.
ACCESSION   DAWAOG010000454 DAWAOG010000000
VERSION     DAWAOG010000454.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8498_length_1816_cov_1.939807"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          531 a          429 c          331 g          525 t
ORIGIN      
        1 tttatccata tataaataac tcagttcaga tacataaatc cctgtatcac aacacctatg
       61 atcataataa gcgcaagtac ccccgcacct atcttcatac ctattccgag cttttcgcga
      121 tttttctgct tcatattccg ctctcctgac cttataaatt tatatagcta attatacacc
      181 gataaatttc tgctgtcaag tgataatgta cctgcataac ccgcacaatg tcccgaataa
      241 actgtatcat taacttttcg gagtgtgata ttatgtttat aacgatactg caaacagctt
      301 tacaaagcct tatatttttt gcattgcatt tcagaagcaa cggctcgttt ccccgtcagc
      361 tttccgctaa ggaagagcgg gaatgccttg aaaaggcggc tcagggcgac cttgccgccc
      421 gcaacaagct tatcgatcat aacctccgcc ttgttgcgtt tatagtaaag aagcactacc
      481 ccgacagcag ggaagccgat gacctgatat ccatcggaat aatcggactt atccgcgccg
      541 ccgaaacatt tgattataaa aagaacatca acttttcgac ctatgcggga aagtgtataa
      601 ataaccagat aagaatgtat ttccgcaaaa caaagcacct acagacggaa gtatatatga
      661 acgagcccat ggactgcgac aaggacggaa acgcagtcac cctcgccgat atcttctgcg
      721 acggtacaga tgttgccgaa gaagccgcac tgaatataga acttgacaga atgtacagat
      781 atatagacga agagcttgac gagcgcgaaa aggagatact tacaaagcgc tacggcttgt
      841 cgggtatgtc ctacagcacc gacaggataa tgacccagcg tgaggtggca gacgagctcg
      901 acatcagccg cagttatgtt tcaagaatag agaaaaaagc acttgaaaag ctccgtgccc
      961 gctttgaaaa gggtgactga gcctgtttgc aaattccgat acaaccactg cggtatttct
     1021 gtagccactt aatcaaagca atcaataata tcctgtgcgg taaacaaatt gagtcgatct
     1081 ttattttgcg ggtcactcag atcaattgca ctctgttctg aattcctgac gaaagcgtca
     1141 attccgtttt cagtcagtaa cgaaaacaga taagtttttc ctatttcaat ttcaggtctg
     1201 ttaaacgcta ttgttattgt atctgaaccg cccaacgctt caagctgttc ttctatgaac
     1261 atatcttcaa aaccgccctc tatccaaagg tttaactttt ccgggacatt atcgttaatg
     1321 tacttataag gcgttatcac ttctaccgtg taaatagtaa atatttcttt gtcttcctgc
     1381 gcggtttcat cggttgcgac ctcatagcta tttctgtcta ccgctacgaa agtaaaccct
     1441 gtgacctttc cggacactac aacttttgac gcatctacca gctctttagc tgtattatat
     1501 gcttgataac atgaattgac tgcattatat cttacatttc tttgagagct ttcgttatca
     1561 agtgatgaaa cggcgctcga aagttttttg tcctcacagc ctgagagtaa aatcaacgca
     1621 aacatacata tgaaacatat catctttttc atgcatattc cgcctttctg cctcggttta
     1681 tatttcctta tcttctactt acaagtattt tgccatattc cttataagcc ctcagcttgc
     1741 cgtcataagt aaaatacaga acatcgttgc gatcaccgtc accgaattca tattcataag
     1801 cgttcacagt agacgg
//
LOCUS       DAWAOG010000455         1816 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8496_length_1816_cov_2.432141, whole genome shotgun sequence.
ACCESSION   DAWAOG010000455 DAWAOG010000000
VERSION     DAWAOG010000455.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8496_length_1816_cov_2.432141"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    511..520
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          465 a          437 c          334 g          570 t
ORIGIN      
        1 atctgaccgt atataaaaat cagcataaac agcacaagag atgacagcat cggtaatacc
       61 atttttacaa cataaccgaa gtcgttttcg gtaggctctc ccgcaaccga ctggtagacc
      121 gaaaccgtct tgtttacagc cgtgatctgt tcatcggtaa gccctgccct tgacagcctt
      181 acactgtcaa aaatactctt tataatttct ccgaaatcgt ttgctgtccc cgatgaaaca
      241 gaatcatcac ctggacgcga tgcataaata tcataaccgc ttttattctc tttgatcgtt
      301 atcagcaatg acttgctttc atttttcact ttttcggtaa atgacggaat atctgcatcc
      361 ttgccgcaat catttatttt tacgccgatg cctgataaaa agccctcata gtccttgccg
      421 gaggttattc ccgttgtatc ggaaaaatat acggtgttta caggcggctt gccatctacc
      481 gtcacatcac cgtcatcgga cgttgcacgg nnnnnnnnnn ttcatatttc accactcctt
      541 ccggcttcct tatcggcttt ttctatgttt tcaacgaata tctcatgcag gctcggctct
      601 ctgagctcat agtgtacaag cggcataccg ctgtctataa tgagtttaag aagcttaccc
      661 gcctgatcct cagactgtat tttaaatgta agtttatcgg gagtgatagt ttcgggtaca
      721 agcccgcact gccgccaggg gaagtacatt accgtcacac ttaacggaca ggttcactct
      781 gccgtagcct ttcttgatct cgttgagatc gccctgaagc accgcagaac catggtgcag
      841 tatcgttata tccttgcaga actcctcaat gacaggcatc tgatggcatg acattataat
      901 atactttttc ttttcgatct gctcgtttat aacgctcttg aaaagatcgg cattaacagg
      961 atcaagaccg ctgagaggtt catcaagtat aagaagctcc gggtctgcaa caagcgccgc
     1021 aacaagctgt atcttttgct gattaccctt tgacagctgc tctgctctct tgtcctcata
     1081 gccgttaagt tcaagccttg caaaccagta cttcagtgac ttttcgatat cggatttctg
     1141 cattcccctg agttttgcaa aatatgtaag ctgttcgttt atcttgtact taggataaag
     1201 acctctttct tctgcaaggt atccgacttt ttcggtgaca ggcgataatg ccttaccgtt
     1261 ccacagcacc tcgccgctgt tgcggctgag catacccagt attattctta tggcagtagt
     1321 tttacctgca ccgtttgtac ctaacagcgc ataaactcct ggttcattca gcgtaaagct
     1381 gagcggatgc agagcagtaa agctgccata ctgttttgat atctctttta cttcaagtgc
     1441 catatcgaaa ttttgccttt cttttcagaa tttgctttac cgccatgaat acttaatgtg
     1501 ataagtatat cacaaatcac agcgtttagc aactaaaagc cacaacagcc cttcaaaaaa
     1561 gcactgttaa tgtgttatat taacagtata atcactttgt gccgatttgt caatacattt
     1621 ttgaaaaaat tttcgttaat tcgtactgat attaaaaact aaagttttta atcagcttgt
     1681 agaaaaagtc tgtttttaaa ataaacttct gatttattat ccctatcccc tttaggacac
     1741 tctgatggct ttgcatcgtg caaagcttgg tgtgtcctta tttcggcatc cttgccgaaa
     1801 gcccctttcc cccggt
//
LOCUS       DAWAOG010000456         1806 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8554_length_1806_cov_2.500857, whole genome shotgun sequence.
ACCESSION   DAWAOG010000456 DAWAOG010000000
VERSION     DAWAOG010000456.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8554_length_1806_cov_2.500857"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          462 a          428 c          309 g          607 t
ORIGIN      
        1 ctgtaataat ccgcaagttc tatcataccg cgtataccgt tgataggcgt tcttatatcg
       61 tggctcatac gcctcagaaa atcactcttt gccatatttg cgcgtcttgc ttcatctgcc
      121 gactgaagca gtttagcgtt gtatttttcc tccgcaatgc gtgtttcacg gatatgcttt
      181 acctgtattg acctgtaaaa cagtattgcc acaaaacaga ttattatata aattgtagtt
      241 gcaagcgagg tcgctgccca tcgcccgata tatacattgc ctgccggata acagacatag
      301 atgtaataat ttctgcactt atccattccg ccgtaatacg agccgtcatc gtcaactata
      361 cgcaaaagac cgttcgcttt atcgccgctc aaagcatagc tttccaatat tttgcaatct
      421 gcaactgctt ttccggtgta tttttcatta ttagtcgcta ttacgttgct tttgtctgtt
      481 ataattatac ttccgttcat tgcaagctta aagcctgaca gcaatgagtc cagtgtcggc
      541 tgaccgttat ctattacaga ttttgtctgt ctgagatatg caaatactat acctttttta
      601 tcggtgcggg caactgccgc aaaatcgtaa taataatcgt tatcgcttgt agctccggac
      661 ggagatgagt gtattctgtc tgcatatatt ttaatcggtt catatgcaac actcaataca
      721 ttatcctgat gtataaagct cttccatacg tcatatcccg tgtcatcgct gtataattca
      781 ttctcaagct cccctttttc gtccagcagc ataaccccgg taagcttgtg atcgccgata
      841 aaactcttca tgatattatc cgagctatcg ttgaatataa gcatatcacg gcttatctcc
      901 ttcgttttat caataatgcg gaacaggctc ttggtaaggt ctgacgatgt tatctcatca
      961 taccttatac actgattttt tatatactct attgtttctg cggtaacatt ttttgcattt
     1021 tctgtattat tatatgaaaa cagtgcaaaa acacagccca tcagtataat acccactgtg
     1081 accattacga taatatgttt aggaaagagc aaatttttat tctttttatt caatcactat
     1141 tcctccaaat gccgcttcgt tatcgagtcc gtatttctcg gcgatttttg caattgttcc
     1201 gtctttcagc atatctttca gcacagaatt gagtctgtca ggaatttctg tgttgttgtc
     1261 tttataaaaa gctacaccga gctttgaaat caacaggttt tcatcaagca atctgtattt
     1321 tccgtcatca tcatttacaa gctgatggag cataaattca tgacccgcta ttgcatctac
     1381 atatccttta ttaagcgccg caaatatgta atccgttttt acaaaacaat aaagagcacc
     1441 gacctgcggc atattgttgc ttttgcctga caacagctct tccggctttg agctggactg
     1501 taccgcgacg ctttttcccg caagatcgga cagcttgtaa atatcgctgt ctgctctgac
     1561 agcgaccgct tgcctgctgt tcatatacgg acctgcccat tggtacatat tttctctgcc
     1621 gttcatagaa aaacatcccc acagacagtc gacctcaccg ttagccagat aactgtcctt
     1681 ctcgtcccat acaatatttt tgaatttagc tttatagccc attctgccaa aagcttccgt
     1741 tgcaatttca acatcaatgc cggcaggttc tccgttttta tcgctgaaat tgtacggctc
     1801 atattc
//
LOCUS       DAWAOG010000457         1801 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8573_length_1801_cov_1.924399, whole genome shotgun sequence.
ACCESSION   DAWAOG010000457 DAWAOG010000000
VERSION     DAWAOG010000457.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8573_length_1801_cov_1.924399"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          523 a          373 c          462 g          443 t
ORIGIN      
        1 aagcaatgga gaagaattac acggcaggct acattaagga cgccgattac tgctttgaaa
       61 tgttgtccga atataaaaaa gccgacaacc tgacggtgga acagaaaaac cggcggactg
      121 agatactgac gaatctggcg gcaaaaggac tgtttgtcgg cggacttccg gatgtgaaag
      181 aaaacgctgc agcaatggcg gatgttatag agaatgcgga gtcttaccgg aacagctttg
      241 aatatgccga gctgctcgca aaaatgcgtg tcggcgtcca aaacggtacc agcattgccg
      301 attccgtcgg cacaggtatt acctattcta tcgtggacaa tgcgattgaa aaggcactga
      361 aaaatccgga ggactttgtg tggaattaca tagcggtcat gttgtataac aatcaggatc
      421 gtcataccct gtaccttact tcggaatatt cagctgactt tcagattctc actgtcgttg
      481 aaaattttga aaatcagttt aaaaaacagc tcggaaataa tattaccaag gaagaacagc
      541 ttggaattaa gaaactggtc atgcagacct atctgcgggc gttggcgctg gataaatgcg
      601 cggattacag attggaagcg ctgaaaagcc ttgataatcc ctatatccgg gaaaacacca
      661 tgtatgctct tttgaaaacc ggtcggtacg atgagtgttt aaagcttgct aaggagacgg
      721 aaaccggaac caatcccgca cctatttact atgcggcagc ggctacactt gaaagtgaag
      781 actttgatgc ttccgtagaa tatgcgctgg agcttgcaaa gcaggcaaag tttagcgaat
      841 atcccaaaaa ggcagatgag ttgttacatt cctggcttgc attgctgata gtagatacaa
      901 aaaccacaaa agcgaagtac ggtcaattga gcgaggtaca gataagcagg cttgaaagcg
      961 atgcgttttt gaaaaactat cttgaagcat atgcgaacgc ctactattcg acctacggcg
     1021 ggcactccgt ttacgacgat tcctatacaa cggcactcga taaggcgatt gccgccgcgg
     1081 accgagtact gtccgagcgc ggcgaattgt gctatccgta ttacctcaaa ggtgtagcgt
     1141 ttgcacagca ggaaaaattt gaggatgccg tgggcgctta caaaaaagcg attgaataca
     1201 agagcgatga cccgatgata tggtatgcgc ttcattccgc atatgttgag ctggaggact
     1261 gggagaacgc atataccgcc gccgaaattg ccattggtct gagtccgtgg tttaattact
     1321 acaatgatta cgagggcatc ggtattcata tgtattccta ccgcgatcgt gcgaaagcca
     1381 tgctggaaaa acagcacgaa gcatcacaga agggaggaaa cgaataatgc ttattatact
     1441 tttatctatc gtatttcttg ttttgcttat gcaaacattc tttctcggcg gcactgtttg
     1501 gctgggtgtt atcgggatgc tgctttgtgc agcgggagta gcagcgtcga ttattctgaa
     1561 aaagcaagga aaaaatcgct ttggcacgat atcggcatcg gcgggactgg ttcttgcggt
     1621 tgtatgctgc ctgttcatta cgtccggcgt cggtatgggt accttacagc aaaaggaggc
     1681 gcttctgaag aagcttgacg ttgccaaaaa tgtagaagca gccgaagaaa aatacaattc
     1741 ttatattgca gtgtacgggg aggacgattc ctccgcactc tgtctggcaa aatactatat
     1801 g
//
LOCUS       DAWAOG010000458         1797 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8592_length_1797_cov_2.530999, whole genome shotgun sequence.
ACCESSION   DAWAOG010000458 DAWAOG010000000
VERSION     DAWAOG010000458.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8592_length_1797_cov_2.530999"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    819..828
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          469 a          401 c          411 g          506 t
ORIGIN      
        1 tagggataga gaatttgctc catttttata aaaatggact ttttctacaa gctgagaccc
       61 cgctcaaaag agcggggtca gctttatgtt ggttttatct gtcttttttg agcattgata
      121 cgccgatagc aactgttgct atgccggaaa cgatgtatcc gatgaccaga tcgctgataa
      181 acgtacctgt cattgagtcc ataccttcaa gtcccgtcag aaggtcgacc tgtttcataa
      241 ggtcaaacag accgaagaac acactgaaga aaccttcata acccagtaga gaaagagctt
      301 catctattcc cggttcgctt actatctgcc atgcacatat gaacatattg ctcagcagta
      361 ccattataag cactattacg atagatgtga taagacccag aacatcaaac ttctttccga
      421 acaggtgata tccgaatata gttgagaatg ccattgcaac gccgccgaga tatgaaatat
      481 atccgagctt tcctataatt atccatatcg cacagccgat aagcgagaag agcagagcgc
      541 cgaggatacc cataagcggc ttgctttcct gaactatcgc ggtgggtctg cctgctacaa
      601 aacgaccgtc attggcaaac gagctcatag gctgttgtac aaagccgccg ttcattggct
      661 gctgtacaaa gcctccgtta ttattcatcg gtgaatatgc aggctgcgaa aagccaccat
      721 tgataggagg gagattgccg tttgtcgggg gctttagctc tgcaagtgac tgagcggttg
      781 aatatcccgt actttcgggt gctgttgcag ggtcgattnn nnnnnnnncg ccattgcgtc
      841 attgcccgac tgagaagagg gaagcggagt accgcccata gccggtataa cattttccgt
      901 ctgaaagtta ttcgtaacag gcggtacagg tgcgccgcct ataggcactc cgtatactcc
      961 tgtatcaggc tgaggtacag gctgagcctg attaaatgtc tgctgatcat tcgtcgcagg
     1021 ctgtgccgga ttgaatgacg gctgattgtt gatagcaggc tgaacaggtg ttgtctgttg
     1081 agctgatact gccttttcag cctcgtcttc ttcgctctgc acacacattt tgcacagctg
     1141 ctttatattg ccgtcagccg cctttgcaaa gcctagtccg attgaagagt tgcactttgc
     1201 gcaggcgttg ctgtaataat ttgcagagaa gtatttaact atatcggtaa taaagcgctt
     1261 tatatccgat gtggttttat tgtcgtcact tcccgcaaaa agaaccgctg agatgtttgg
     1321 gtcactcacc ttataagcct tgacaaagct gttttcgggc tttgccatat attcggtgaa
     1381 aaatccgtct gccgttttac ttgtgagcgc gctttttcgc acccaagagt tgaatatgat
     1441 acagctcttg tcgcttagtg ctcggagagt gaagccgtat ccgacataag tgccgccggc
     1501 ataatcttcg gcataaagag tcagtgcaag ttccggcttt aaatgtaaaa gtacgtcagt
     1561 taccattata tttcctccaa agtttgcttt agcttgatcg taaggcaaaa ccttaccagt
     1621 taattttact ataatcaggc gttcgtgtca atagataaaa gcgatgggac aagtaaaatc
     1681 agtgagttta taaaaaaagc tccccttagg ggagcgcaga ctgtagaaaa agtctgtttt
     1741 tgaaataaac ttctgattta ttatccctat cccctttagg acactctgat ggctttg
//
LOCUS       DAWAOG010000459         1789 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8650_length_1789_cov_1.977509, whole genome shotgun sequence.
ACCESSION   DAWAOG010000459 DAWAOG010000000
VERSION     DAWAOG010000459.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8650_length_1789_cov_1.977509"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          620 a          360 c          424 g          385 t
ORIGIN      
        1 ttacagcagg atgcggatta cggtgatgat agcgatatcg gtatcaatga gaacgacgag
       61 gaataaattg cttttctctg cggatacttt taccgacagg atacagcaaa aacggaggac
      121 ttatgggctg gcttattttt attgatatat tgctcatcat agtgctcgcg cttatgctgt
      181 ccgtaacggt gaacgtgcac cttaacgatg aaacaaggat aaagatcaaa tatgcgggga
      241 taacggtatt ttcggtatct cccgaagccg gaaaacagcg taagcaaaaa caggaaaaga
      301 aaaagggaaa gagcaaaccc gaacctgtta agaaagcccc cgcttcaaca acaggcgaaa
      361 atcccgccga aaaagagaca agcaacagtg taagctcaaa aagtatcggt aataaatcaa
      421 acgacaaaga caacaccgaa accgtgaata agcccgaaaa gaaaaccgga aaaaagccaa
      481 aagacaaaga aagctttatg tcaaagctcg gactgagcgg cgacataagc gaaacgatag
      541 aatttgtaaa acagcttatc agccacataa aagtaaacaa atttacactt ttgattactg
      601 tcggcggtga agatgctgca cagacggcaa tgaactacgg caagataaac tggcttgttt
      661 ataccgcaat tgcagtgctg tatgaagcgg tgaagcttga tgtcaaaaaa atagacataa
      721 cagccgattt caattcaggc aaaacagaat atgagctttt gtgcaaggca aaactgcgac
      781 tgggaacagc gctttgctgt atcatatggt tgttattcag attactgaaa atgtatctaa
      841 aattaaattc aaagaatgaa aataaaactg caacggctga aagcaataca aagccaaaag
      901 cagagaaagc ttaagctttc ggaaaggata tcaaaatggc agaacagcac cctatcaacg
      961 gacttatgga cacctcaatg gaaaagataa gagagcttgt tgatgttaac accataatag
     1021 gcgatcctat cacttctccc gacggaacga taataatccc tgtatcaaag gtaagctttg
     1081 gctttgttgc cggcggatcg gatcttccga caaaaactcc taaggaagta tttgcaggcg
     1141 gaagcggagc gggcatcacc ataaatcctc aggctttcat tgttgtacag cgtgacggag
     1201 atgtaaaaat gctggaactc ggtgcaggcg gcggaagcgc tattgaaggg ataatcggcg
     1261 gagtgcctga ccttatttct aaaatcaagg ctatattctc aaaggataac ggcgaagaat
     1321 ccgaagaaaa aacagaagaa taagcaataa taacagctgt ccttttcgca taagatatac
     1381 caaaactatg cggaaaggac aacattatgc acgaaaagat catatcggct gtggtatccg
     1441 cagccttcgt acttatgact gctgtgccgg tatgcgcaca ggaggaaaac aagctttgct
     1501 atcttgaaaa taccgataaa gcggagtttg aggtgtctgc cgtaagcgct attctgatag
     1561 aagcggatac cgcaacggta ctcttttcaa agaatgaaaa ggaacacaga cagatagcaa
     1621 gcactaccaa gataatgacc gccctgctca cccttgaagc gggagaaccc gacaaggaat
     1681 ttcgggtcga ctctgcggcg ataaaggtag agggtacttc tatgggactg cgtgagggcg
     1741 ataaagtaac acgcagagcc ttgtgttacg gtctgctcct gccctcggg
//
LOCUS       DAWAOG010000460         1781 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8702_length_1781_cov_2.093279, whole genome shotgun sequence.
ACCESSION   DAWAOG010000460 DAWAOG010000000
VERSION     DAWAOG010000460.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8702_length_1781_cov_2.093279"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    241..359
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          395 a          412 c          349 g          506 t
ORIGIN      
        1 ggtatgcata aatttcagag gaattgacag cgtacacgct ttcgcgcccg acttgttcac
       61 actcatcgta tcggcatttg tgcctgtttc tccgcccata acctcaagct gatacggaat
      121 attctccgtc ttagcaagag ctatcagact gttcgacagc tctcttgaaa gtgatggcgc
      181 tatgcctatc ataacgccct ttgacatatc ggcacatttt gccctgtcac tgtcgggagt
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
      361 ctttaagcat ttcaagagca accagtatag aagctactcc gcacctgtcg tcaagcgcac
      421 ctgcacagta ttttgtgccg agcatagctg taggctcaca gtcaaatgtt atcctgtcgc
      481 caagctcaat acggctttca agctcagatt tcgtgtagcc tgtgtcaata acaagctctt
      541 ctattttcgg cactccgtca ccgctcttta catgaggagg aagaacggat acaacgccct
      601 ttaccttttc cttgccgtgg atcgtcacgc tctgcgccgc aagcacacgt ctgtcaggac
      661 cgcctaccgc gccgaccttt ataaatccgt tgtcgtctat aaaggatacg ataagtccga
      721 ccctgtcgat atgtgcgtca agcagaagcc taggcttatt gcctgtgttt ttgatatgtc
      781 cgataacgtt tccgaaatcg tcaatgttgc agtcgtccgt gtagctttca agcatagaca
      841 gcgcaatttc ggacgcactt ttttcatcgc ccgaaacacc gtcagcactg cacagactgt
      901 acagcatact gttaatatcc attgtcatct ttctttcata gcttaatata agccgtccat
      961 tttgatcttt tccttgactc tttcaagctc ggctataaac tgctcgtcaa gcttgcttaa
     1021 gctgtagccc ttggggaaga taagcacgtc tttatttacc ttgtagggaa tgtcacattt
     1081 cagctgaacc attccgtgct gttcaagaca gcttttggga acgggagaaa cccacatata
     1141 tgtgttgtgc aggtttccga gcaggtcaaa ctggcttcct ctttcataaa catagatacg
     1201 ctttttacga tgctctgcgg catttgccct tgataccttt gagaatgaga gagatggtgt
     1261 cgaaagatcg ccgtgtacta tctcgatgta atcctcaagg tcggaatatt ttactatact
     1321 gtccggattt tcctttgcga gcgggctgtc cttattcaca agaagcttgt attcaaactc
     1381 ccataaaggg cggtatttca gattttttcc gtcaagcatg gcaaaatatt gcttttcgtg
     1441 gatattctga tacctgatta tgcccagctt gaaatcaaaa ttcatgatgt tgtttattga
     1501 agttgatgga tatctccttg tcggtgtcaa gcaggctcac aaagtttgaa aatgcctttg
     1561 tgatgtaggt agctctgggt actgagatgc tgaaacgcac agcatcatcg accttaggct
     1621 tgtacagtga taccatttca tcaaactggt caagcaccgc ctttgcatag cctaaaaatt
     1681 cgccgccttt ttccgtcgga gcgaccccct ttgtcgtcct gcggaatatc gtgataccta
     1741 tttcgttttc cagttctttt atagccttac tgagattagg c
//
LOCUS       DAWAOG010000461         1779 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8717_length_1779_cov_2.204176, whole genome shotgun sequence.
ACCESSION   DAWAOG010000461 DAWAOG010000000
VERSION     DAWAOG010000461.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8717_length_1779_cov_2.204176"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          547 a          351 c          453 g          428 t
ORIGIN      
        1 catcaaggct tccgctgttg atgaagaaac aggcgaagaa acaatggcag ctgtcacagc
       61 tgagggtgcc atggttttca acgttgatat cgctgacctt ggtacagctt taggtattgg
      121 taaggactgc gaaggttacg atgacctcaa gaccggcgct gagaagatgg aattcgctaa
      181 gtcaaagggc attaacgtaa cagacgttaa gattacacag gttaaggacg gcgaaacaac
      241 agaagttgct gttgattcaa acaagattct ctttggtgat atcgaaggta acggcaagct
      301 ccgtatcgag ctttacaacg agtacggcga tacaaagaat gacgctcctt taaaggtttc
      361 tgacctcttc ttcaatgaga agcttgaagt aacattcaca atctcaggta tcgacgctat
      421 cctgaacggc ggcagcacaa ctcctgatga aacagacgct cccgctacag atgctcccgc
      481 tacagatgct cctacagccg gtgattctac aaaggacaac acaaacacag gtgttgaagg
      541 cgtagcagca gttgcaggcg ttgctatcgt agcagcaggt gcagttatcg ttgctaagaa
      601 gagaaagtaa tttctgttca cggtaaagta cattaaaatg taacataagc ccacgcattt
      661 cggtgcgtgg gctttttgtg tatgattttt cataaagcaa cgggggaggt tgtgatacaa
      721 ttcctcggct tatcatgttt cactgaaatt gcaatgcaac atattgtaaa aaagtcagaa
      781 caataaatat tgtgctagca tcgctgaaaa cagtttctat accaataaga gaaaaacgtt
      841 ttttgatgag ctttcctcaa cagccggcga caatcacgga gcgaaggttt acggaacgcg
      901 ctgaagcgaa ttgccgcaaa ataaagcttt atccccgaaa atttggggga attttctata
      961 tttttaagaa aaaatattga caaaatcaaa aagcgtgcta tactgtgatt agtgtatata
     1021 cacagattac aaggagaaga ctatgaatat cattttaagc aacagttcaa acgagtcgat
     1081 ttatgcgcag attgaggctc aagttaagca gcagataatg agtggggagc tttcgcccgg
     1141 ggaagctctg ccgtcgatga gaacgcttgc gtcgcagctc aggataagcg ttatcacgac
     1201 aaaacgcgcc tacgaggagc ttgagcgcga tggctttatc gagaacttcg ccggaaaggg
     1261 ctgctttgta aagcggcaga acacggattt tctgcgtgaa gaagcggttc gtcagaccga
     1321 agagcttctg gggaaagtct gcgaaaaggc gaggatttgc ggtctaaccg cagatgagct
     1381 taaggaaatg attgacatta tttacggagg agaatgaaat gtgcgattat cagaacgcaa
     1441 ttgaaatcag cggcttaacg aagcactatg acgggttcac gctcgacaat gtgagcttta
     1501 gtgtgcccgt tgggagcata atggggttta tcggacaaaa cggcgcggga aaatccacga
     1561 caataaaggc gattttgaat atcatcgggg ttgacggcgg cgaaataaag ctgctcggaa
     1621 aagattacat taaggacgaa tacgaggtaa aatcgcagat tgctgcggtt ttcgacgagc
     1681 ttccgttcta cgacaaattc aacgcagtgc agataaacac gctgtttcgc gggctttaca
     1741 agacttggga cagcaaaacg tacttcggtt atatagagc
//
LOCUS       DAWAOG010000462         1774 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8743_length_1774_cov_2.218150, whole genome shotgun sequence.
ACCESSION   DAWAOG010000462 DAWAOG010000000
VERSION     DAWAOG010000462.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8743_length_1774_cov_2.218150"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          487 a          358 c          513 g          416 t
ORIGIN      
        1 ggcaccggca gcgcggatct tgataccggc atcggatttt tcgaccatat gctgacggcg
       61 cttaccgtac acggcggatt tgatttaaag ctcaagtgtg acggcgatct gtacgttgac
      121 ggacatcaca cggtagagga ttgcggaata gtgcttggca aggcactggc tgaggcgctt
      181 ggcgacaaga gcggtatcgt gcgtttcgga agcgcatatg ttcctatgga cgaggcgctc
      241 tcattctgtg cggtcgatat aagcggcaga ccttatcttg tgtttgacgc ggaatttacc
      301 gatgacagaa cgggtatgtt cgataactgc cttacggagg agtttttccg tgcgtttgct
      361 ttcaatgcgg gaattacgct tcacgttaaa aatgtttacg gcaggaacga ccaccacaag
      421 atagagtcga tgttcaaggc ggtggcttat gcacttaaaa cagccgtaag gaaaaattcg
      481 gacggctctg tagtttccac gaaaggaaca ttataatgat aacgataatc gactacggcg
      541 caggaaatct gttcagcgtg cagaacgcat tgaattttct cggtgtggaa aacaatatat
      601 caagtaaggt cgaggatatt gtttcggcgg ataagctgat actccccggt gtgggagcgt
      661 tccccgacgc tatgaagatg cttgacgagg cagggcttac cgaagtgata aaagagcagg
      721 caaagaaaaa gccgcttatg ggtatctgtc ttggaatgca gatgctgttt gacaagagct
      781 atgagttcgg cgaaaccgag gggcttgggc ttataccggg tacggtggag cttatgcacc
      841 ctgcaaacga ccttgtaata ccgcacatag gctggaactc gcttgaaaag aacgagccgt
      901 gcaagctgct cgaaagcgta aatgacggcg attatgtata ttttgtacac tcttatgcgg
      961 ctgtgaccga cagcaagaat gttgcggctt actgcgacta cggtatgaaa gtgcctgcgc
     1021 ttgttatgag cggcaatgta tacggctgtc agttccaccc cgaaaagagc ggcaagacgg
     1081 gactcggaat acttaaaact tttgcgtcac tttaagcggc aagtgagaaa ggagctactt
     1141 atggataagc tgatatgttc atgcggagga aagctgaagc agggattttt ctctggcgga
     1201 ttacttccgt tgcaggctca ggtgtcggtc gaatttgatg gctacggagt agctgtaagt
     1261 ccggtcacgg cttatgtctg cacaaagtgc ggaaaggtag agctgtatgc agataaaact
     1321 gttgtctgcg accccgcaga gcaaggaaag gaaaacgaag gcgaatgata attctacctg
     1381 caattgatat aaaggacggc aactgtgtgc gtcttgtaaa aggcgattac ggcactgcac
     1441 acaaggtagc ggaaagctat atggatactg cgaggagctt tgaacaggca ggctcgcagt
     1501 ggattcacat ggttgacctt gacggagcaa aggacgcaag caccgcaaac aaggagatat
     1561 tccttgatgt agcaaaaaac acaaggctga aagtagaggt cggcggaggg ataagaaacc
     1621 ttgataccgt tgaaatgtat ctttcgggcg gtgtgtcaag agttataatc ggctctgcgg
     1681 ctgtcaaaaa tcctcagctt gtaaaggacg ctgtaaagga atacggcgac agaatagcgg
     1741 taggaattga cgctaagaac ggctatgttg cgac
//
LOCUS       DAWAOG010000463         1772 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8765_length_1772_cov_1.751893, whole genome shotgun sequence.
ACCESSION   DAWAOG010000463 DAWAOG010000000
VERSION     DAWAOG010000463.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8765_length_1772_cov_1.751893"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          537 a          310 c          438 g          487 t
ORIGIN      
        1 tcttaagacc gctaatctgt tcaattgcag atttggtgat gtgagcgagc ttgagaatgc
       61 ggtcagccgc tggaacaagg agctgtccgt caagggcatc agcgtaacag tactgagaag
      121 acgtgaaaac acggcactta tatatgtata cagaacggaa aagcttgcta aagatttcag
      181 tcgtccgggt gtagcgcgtt ttatgaagcg tcacggttat gagaatacca atatcggata
      241 ctgcattgac attctcaggg aaaagcttat gaacagtgag gattttccgc acgagatagg
      301 gatattctta ggatatccgc ttggggacgt tataggcttt atcgataatg caggcaagaa
      361 cagtcggtgc acaggctgtt ggaaagttta ctgcaatgag tgcgaggcta tgcgcacctt
      421 tgcgagattt gacaagtgca gagctgtcta ccaaaagatg tgggcaaaag gcaggagtat
      481 tgtgcagcta acagttgcat aatataaaga acatttatga aaggattttt tattatgaaa
      541 acagcagtag tatattggag cggaacaggc aacaccgagg ctatggcagg ctcggttgca
      601 gagggtgcaa agagcgcagg cgcagaggta agcgtattta cagcggctga cttcacggcg
      661 gatatggtgg atcagtttga ttctatcgct ttcggttgtc cttcaatggg tagcgaacag
      721 cttgaggaca gcgagtttga gcctatgttc aacgcttgtg aagcaaagct ttcaggcaag
      781 aaggtaggac tattcggttc ttacggctgg ggcgacggcg aatggatgag aaactgggag
      841 gaacagtgca tcgctgacgg cgcaattatg gcaggcgaat gtgttatctt caacgaagct
      901 cccgatgaca cagcaaccga gcagtgcgtt gagctcggta aggcgcttgc atcataaatt
      961 cagaatacaa cttaaagata aacaaacata ctccgtaaaa caccgcagag ggtaaaagct
     1021 ctgcggtgtt tttatttata tgaatttatc gggagtgtgt atgcttgaaa taagcacgtc
     1081 aagactgctt ttgatattat gcggaagtgt acgccactgt cgcagatggg taagctcctg
     1141 ctcggaaaga ttatcggacg acctgataat aagcggttca cggatatcgg tatttccgat
     1201 aagataatca accgacacat cgaaaatttc agctaagcgt ataagggtgg agatgtcagg
     1261 ttcattatcg gtcttttcgt acttgaatat tgcctgctgt gatacattga tcatttcggc
     1321 aagcttttgc tgggaataac ccttttcttc tcttaacaac cgtaagttct taaccataac
     1381 aacctcctgc gtttatttta tctttattgt accaaaataa aaagaaaatt tgaaaaacta
     1441 ttttgttgtt gacaacaacg ttaggttgtg atataatgaa gtcgaaaaaa gtaaatacgg
     1501 tgatgattat gttgaaataa tcatatatcc gtgtttggaa atgttggagg gtttttaaat
     1561 ggagaaaatg ttaaaaagct ttgctgttat ctgtgttatg gcaattttaa cagcatgttc
     1621 aaatacagcg gataattcaa atactaacct taactcatct gaaatcaatt cagaaagttc
     1681 atcagcatac agttcatatg cagaaaacag ttcatcatac agctatgaat acagttctga
     1741 agatgattac tactatgatg acagttggga tg
//
LOCUS       DAWAOG010000464         1768 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8795_length_1768_cov_1.799766, whole genome shotgun sequence.
ACCESSION   DAWAOG010000464 DAWAOG010000000
VERSION     DAWAOG010000464.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8795_length_1768_cov_1.799766"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    463..472
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          412 a          460 c          360 g          526 t
ORIGIN      
        1 cgcttacttg ttattacatc gccgttttca tctgcgatta taaatacgtc tattgtttcg
       61 ctgctgtttt caagttcgct gatctttcgt atgatcgtat tcctgtcaaa cgcttggtag
      121 tcgcttaggc acgccgcaaa gtttttaacg atgcatatat cgttatttat gttgttttca
      181 acaatttcga atttctgcgt tacagtcctg tcgatatatt tatcgacctc actgccaagt
      241 gcattcgtag tgttgatcat aaaaaacgag gcaaatataa taaagatcac tgtcattata
      301 acggatacag ccaacagcac ttctgtttta tgtttgattt tctgcacagt gatcccccca
      361 gtcgacctct tgacatgttc gttaaattta ctgtttcata aacaagcact tatttaaata
      421 agagtataac acatttcgtt agaaaaatca acaggcgtac acnnnnnnnn nnaacgcctg
      481 catcaagcag cacctgctca aaaacagagc ctatctcgta ttttatgatg ttctccgcat
      541 cagcggcttt ggcatcggga tatttcttat acagtccgtt catccactcg gcgtgacccg
      601 acagcttttc atcgcccgta aggtcggtct tgtttacaag cgcatcacaa agtgccgtta
      661 tctcgcttgc aagccttgcg gggagcaccg caagccccat tacctcgata agaccgatat
      721 tttccttctt gatgtgatgc agtgacggat ttgggtggaa tataccgagc ggtctttcct
      781 cgctcgtgat attgtttctg agcacaaggt cgcattcata tacatctccg tgccgccttg
      841 cgataggagt gatcgtgtta tgcaaagctc cgtccgtgaa cgcgtaaatt ccgacgcttt
      901 catcgctgta cgctctccac gcaacgagaa ttttatcaca cgccttttca agctctgccc
      961 tgttcttacc gctgagcctg attaccgaca tcggccactt gacgattccc gcctgaacgc
     1021 tgtcaaaacc gctcatctta aattcgtatt caaccggtgc actttccata gcaaatgtat
     1081 agtgtccgcc ctggaaatgc tcatgcgaga ggatagaccc tccgactatc ggcagatccg
     1141 cattcgatcc gacaaagtaa tgcggaagca tatcgattat atcaaacagc ttgccgaaca
     1201 cctccgcgtc tatcttcatc gggatatgct cgctgttgat cactatgcaa tgctcgttgt
     1261 aatagccgta tggcgaatac tgaaatcccc actttccgcc gtggatagtc atgggtatcg
     1321 ggcgcagatt ttgccttgcg gggtgagtaa gcgtacccgc aaatcccgta ttctccatac
     1381 aaagctgaca cgctggataa gaaacctttg cggcatttct cgcggcggcg atatccctgg
     1441 gatctttctc gggctttgag cggtttatcg taatatcaag ctgtccgtac tcgctgtcgt
     1501 acttccagcg tatatccttc gctatcctgc ccttgcggac gtaatttgta tcctcgctgt
     1561 atgtaaaata ccagtcggta gccgctttcg gactttcgcc gtatttttcg cggaatttag
     1621 ctataacctc gtgcggcatt gccgtgaata cacccataat gcgggtatca aacagatccc
     1681 tccacgcatt cgatgcgggt attatctctt tttcgaccgc atagtccaca atttcgtcca
     1741 gtatctcgtc aaccgacccg tactcctt
//
LOCUS       DAWAOG010000465         1767 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8807_length_1767_cov_1.728388, whole genome shotgun sequence.
ACCESSION   DAWAOG010000465 DAWAOG010000000
VERSION     DAWAOG010000465.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8807_length_1767_cov_1.728388"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          380 a          542 c          323 g          522 t
ORIGIN      
        1 gtatgggtgt gggtaaggaa agactgaggg attgtctgat atcaacttat ctttagacaa
       61 actaagccgc gggctgtgca gcttacaccc cgcggctttc tccgtatcaa ctataccatc
      121 agtttcagtg ctcatgcaca tatttcattt gatctgctcc tgcaatatat ccagtatctc
      181 cccgaacctc tgaaaatgta ccacctcgcg ctcacgcagg aactttatca catttgatac
      241 atcgggatca tcgcacgccc tcaatatatt gtcgtaggta gctcttgctt tctgttctgc
      301 cgcaagatcc tcatgcagat ctgcaagcgg gtctgcctta ctctgcaagt acgccgccgt
      361 aaacggaact ccgcccgcat ttgcaggata taccgcatta gcgtgatcca tataatacgc
      421 gcccactccg tacctgtcaa agcttttcgc tccctcgccc gttgtcagct gagaaactat
      481 tgatccgatc atctctaagt gagagagttc ttccgtacca ccctcctccc cgaaatgatc
      541 cctaccgtct gaaaatttcc ccgaaagccg ccttatagct ctcccctctg ccgctcaccg
      601 taaacctcgc cgcaaaacct ttttccacgc ccttgaaata aacagccagc tccttatgag
      661 aatactcgac cctttccact gcccgccata cgcattcatc atcaaaccgc aagatctcac
      721 cgattttcct ggctatgcat ttactgcttt gttctgcggc atctcccata tctgcttttc
      781 tgtcaagctc tttcagctta ctgtcataat actgcttttg ctccgccagc tcctcctttg
      841 ttataagccc gtcaagtacc atatcaacaa gccgccgctt cttctcacgt ataatatcct
      901 tctgcctttg aagcgccgcc ttatccgtta tttgcaccgg taagcttttc attaccctgt
      961 ccgtgatagc cgccttatct gtcacaatgc cgtcaagcat atactttatc cccgccgtca
     1021 gcaccttcac attgagcgtg tcattgtcac acccgttacc ttttatcctg ccggcacacc
     1081 tgagagcttt atatatgccc tcattcagct ttttcaccct cgacaccata acacagccgc
     1141 ataatgcaca gtggaccttg ccgctgtatg gatagcgatc gctgttacga cttgtttctg
     1201 tcggagtacg gcgggagatt tcaagctgag tcatatccca tagctcacgg ctgattatcg
     1261 gctcatggtg acatctcaga tatatcctgc cgcccctgtt gagctttttc ttatgcgtaa
     1321 gataatccgg cgtacagctc tttttctgtt cgagatcgcc gacatatttt tcattgcgga
     1381 gtatcctgcg tatatacgat gcgctccaac tgcctgcgca aggcgcaggt actttttccg
     1441 agttaagctc tttcgctatt gtgcctgctc cttttaaaag aagcgtatat tcgttgaata
     1501 tcctttgcac gatgctcgca ccccgttttt ctatgcttat ctttcctccg ctgacatcat
     1561 accccagaca attcctgcca aatacgaccc cctgctccat ttttctcgcc tgtccccacc
     1621 gtaccctctc ggatattttg cggctttctt cctgtgcgaa agatgcaagg atagtcagca
     1681 tcaactcgcc gtcaccactc atggaattga tgtgttcttt ctcgaaacgc acctccacgc
     1741 cgatatcctt caaatgccgt accgttt
//
LOCUS       DAWAOG010000466         1767 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8805_length_1767_cov_1.977804, whole genome shotgun sequence.
ACCESSION   DAWAOG010000466 DAWAOG010000000
VERSION     DAWAOG010000466.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8805_length_1767_cov_1.977804"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          595 a          320 c          368 g          484 t
ORIGIN      
        1 gtgcaggact taataaattc gatgacaggt gcagggttat ctgtaaggga aatggaagaa
       61 ttgtaggcag aaaacgcttc attctggttt tcatacgagg aactaatcaa acagacctcg
      121 gaaaatcttg ataaagtaaa caactgggaa tacaatccta tggcggcgtt acctgcttgg
      181 atttccattg ttgctcaaaa ataaaataat tgcgttataa aatatacaac agccgcattc
      241 agattttccg aatgcggttg taattttaca ttgaaatttt ccgaaatccg cggtataata
      301 atcccataaa gcaatcaaag gagaacagcc tatgcttata aacaataacg agtatcttga
      361 tattgtcgaa acaattaagc ttgaaataaa aagcgcacaa tacaaagccg ctgtaagcgt
      421 aaacagagag ctgattatgt tatattacaa catcggcaag attatcaatg agcataagac
      481 ttggggaaat aaatttatcg aaaaccttgc cgccgatatc cgcatatcgt tccccggcac
      541 aaagggctat tccataagaa atctgaagta tatggctaaa tttgcggctg aatatcccga
      601 cgagcaattt gtgcagacag tgtctgcaca aattccgtgg tcacacaact gcgctatttt
      661 agataaggtt aaagagtttt caaaacgtga atggtatatc agaaaaacct gtgaaaacgg
      721 ttggtcgcac agcgtactta ttcatcagat agaaagcggc ttgtacgagc gacaggcaac
      781 ggcagagaaa atttcaaatt ttgaaagcca ccttgccgct ccacaaagcg agcttgctgt
      841 tcaaacgatg aaagacccgt atgtgtttga ttttattccg ttcaaagagg atatggttga
      901 aaggaatatt gaggaggctc ttgtcaagga cgttacaaag ctgctgcttg agcttggaac
      961 aggctttgcc tttctcggaa atcagtaccg tctgaacgta ggcggagatg atttttacat
     1021 tgatttgctg ttctacaacc tcaatcttcg ctgttatgta gtcatagaat taaagacagg
     1081 tgaattcaag cccgaatttg caggtcagct taatttctat ctttcggctg ttgacggaat
     1141 actgaagcgt gatgaggata acccctctat cggcttgctc ctttgtaaga gtaaaaataa
     1201 tcttgttgcg gagtatgctc tcaaggatat gtcaaagcct atgggcgtca gcgaatatac
     1261 cataacaaag agcctgccca aagagctgga aaagcagctg ccgtctgttg agaatattga
     1321 aagcaggatt aagaaataag atttacagcc gcagacggat ttgccgaatg cgtcgtatga
     1381 tgcacaaaat ttgctatgaa aggatttaaa aatgtattta taccactatt atgaaagtac
     1441 aggcttgccg tttgcaaatt tatctgattt atcggtaaat gaagcgaatg ctgtactgaa
     1501 taaaataaaa aaagataagc ccaattctca gcacgcacaa agacacgaaa aatatgttga
     1561 atacagaaga aactgcgaaa gcatacttcg cagcagattt attgaaaaag gcggtgttat
     1621 caaaaaaata atccttatta tatgactatt gaacatagtc cttggttaag cacttggttt
     1681 gagaattccg cattcatcaa aattcctatt gaagaatttg atataaagac attatcattt
     1741 acatacggtg attcaatgcc tacattc
//
LOCUS       DAWAOG010000467         1764 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8826_length_1764_cov_2.358689, whole genome shotgun sequence.
ACCESSION   DAWAOG010000467 DAWAOG010000000
VERSION     DAWAOG010000467.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8826_length_1764_cov_2.358689"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          417 a          452 c          383 g          512 t
ORIGIN      
        1 ctcttgcata gtacagcaaa ggtgtatcac atctccagca gtgaggatag ctatgctcga
       61 acttcggagc gtcaaacagc aagccctttt cgtcaaggtc tttaagcacc atagggtcgg
      121 ctttctttac gaatacgcct gcgtaaggtg tttcggcagt catattgccc ttgccgtcaa
      181 cgagctgtac gaaaggcagg tcgtattttc tgccgacctt agcgtcgtct tcaccgaaag
      241 cgggagcaat atgaacaaca cctgtaccgt ctgtaagtgt tacataggtg tcgcatacta
      301 catagtagcc ctttttatgt tgttttgcac agatatcaac agcgcagtcg aataaaggct
      361 catactcctt gccctcaagc tccttaccgg tatatctttc aacaacctca tagggggctg
      421 tgggcttgtc gccgccgaga attttatcgc acagagcctc tgcaagataa tatgtgtagc
      481 cgtctgctgt ctttaccttt acataggttt cgtcggggtt tacgcagagt gcaacgtttg
      541 agggaagcgt ccagggagtt gttgtccatg cgaggatata agcgtcctcg tccttaacct
      601 tgaagcgtac gaccgcagaa cgttccttta tgtccttata gccctgagct acctcgtggc
      661 ttgaaagcgg agttccgcaa cgggggcagt agggaactat cttgaagccc ttgtataaaa
      721 gacccttttc ccatatctgc tttaatgccc accactcaga ctcgatgaag ttgttgtcgt
      781 atgttacata cggatcgtcc atatcagccc agaagccgac agtgcgtgag aattcctccc
      841 acataccctt gtacttccat acgctttcct tacactttgt tatgaacgga tcaagtccgt
      901 acttttcgat ctgatccttg ccgtcaaggc cgagcatctt ctcaacttca agctcaacag
      961 gcagtccgtg agtatcccag cctgccttac ggggaacgtc atagcccttc atagtgcggt
     1021 atctcgggat catatccttg ataacacggg taagaacgtg accgatatgc ggcttaccgt
     1081 tagccgtagg cggaccgtca tagaatgtgt aggtcttgtc gccctttctc tgctccatac
     1141 tcttgcggaa tatgtcgttg tcatgccaga acttttctgt ctgcttttct ctctcgacaa
     1201 aatttaccga ggttggtact ttttcgtaaa gtgacatctt ttctcctcct gtttttgcaa
     1261 ttcaccctgc catatagatc tcttcggcaa aaaccaaaag ctatatgggg ttttgggcga
     1321 aggtctgccc attgcatatt atttattctt tgcgtttgcg gaaaataaaa aagtccttat
     1381 cctccgaaaa ggataaggac tataatttca taacagtctt tataaccacc gtttcgtcat
     1441 tccgtcaaat gcgttccgta taacggcgga aaaccgtccc gttctactct cgcggcgatg
     1501 taccgcaatt tcggcgggca gcttggaggt gattttcata cggtacttcc gcgccgacct
     1561 ctcaccgtcg tcggctcgct ttggcttcat atccgcacta ctgtcctcgt cacagccttt
     1621 aaatatcata catattgatt atacaatcaa attttccctt tgtcaagcgt ttcagcggaa
     1681 aaatatgatt ttattttgca gaaaaatgca atgaaacgtc tgcccgcctt gaaagtcaaa
     1741 acggagagtt cgccgccccg atca
//
LOCUS       DAWAOG010000468         1753 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8891_length_1753_cov_1.696113, whole genome shotgun sequence.
ACCESSION   DAWAOG010000468 DAWAOG010000000
VERSION     DAWAOG010000468.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8891_length_1753_cov_1.696113"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    529..538
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          421 a          500 c          365 g          457 t
ORIGIN      
        1 actggggcta ccgcccctag tccctgtcgg ggggctacgc ccctgagacc ccatttttaa
       61 ataagcaaga ggtttttcga cagactgaaa ccgcaggtac gcctgcggtt tatctgtatc
      121 ttattctatc ttactgcact tccacaattg taatattctc ccctgcgacc tcgacctcag
      181 atagcaatgc gctctttatc tgtgccgcct gagcggtatc gagtccctgc gacttcacaa
      241 tgatattcgc actgttatcg cttaggtagc aaagcacatc ttcaaagccc tgtgccttaa
      301 gcgttgtttc gatcttgttt tcagcctcca taaggtcggt cagctgctgt gcgtcctgtg
      361 cgactaccgc aagctcgtct ttggtcatat ctccgccgcc gtaaatactc ttgagcgttt
      421 ccttagcctc ttctctgctt gcctgcttgt caatgcgcgc ctttgcaaaa tattcctcac
      481 tgctgtcggc taccttttcg gtattggcag aagcggaaac attatcgcnn nnnnnnnnca
      541 acaagatgct ttttgccaag tataagattg attctcggtc ttctcataac aaatacctca
      601 cttatgttgt aatacatatt ctcgatgcgg aaacgtcaag tgccgttttt accgcattga
      661 ttaccctttc ggctatccga ttgcttgaag cttcgggaca taccaccgtc accccgagca
      721 ctgtgtgctt atcctcgtca agcctgaccg tgacattcgg aacatcatct atcccgtcca
      781 tagccgcaag catcagcgtt atgcgctgtt caagcagctg ttcttcggta agctgtgtgt
      841 ctgacccgct gccgaagctt gaaagaaaga taagcgcgat cgccgctatc ccggcaatga
      901 aagctatttt aagcagctta tcggatctca gcagttcttt cataatctac ctttcttttg
      961 cgtatctgac ctttatatcg ttcccgactg ccgactgtac aagtgataca gcatcggcga
     1021 tgtatccatc gtccgtatta tcatcaaata caagctcgac ctcactaata gatatgcaga
     1081 acgcgccgtc aatatgcgct gtaatataca ccttgcggta aacaaatccg tgcgaggtga
     1141 gtagctcctc gaccttttcc ctgacggctc ttgccgcagt gacgcgtgcc tgctcggaaa
     1201 gcttatcgct gaaatccacc tgagggatat cggcgatctt agtttctatg ttaagtgcgg
     1261 gtataacatc ggtgaccgaa tttatcaggc acaccgaaaa aatacacgct atcgcaaatg
     1321 ttatgcaact tgtatttttc acacggtatc agcattctga gcagccccgc cgccgcggca
     1381 agcacgcata taccgagcgc cgcctgcttt atctcgttca taccgcacct cccgccataa
     1441 atatcataag agatatcgat attaccgcca tcagcaggaa cgatacgagc atcgccataa
     1501 ttatagagct aaccccctcg gcgcacttga gaagccccgt gagctgtgat ataccgaaca
     1561 ggtcactgaa cgccgccgcc accgaaaacg caagcttata gcacagcacc gacaatattg
     1621 aaggcagtat caacgccccg ccgactatta tcccgaacac gccgaagttg ttacggataa
     1681 gccctatacc gccgtacata accgacagcg aatctgacac agcgccgccg atgaacggaa
     1741 caccattcga cac
//
LOCUS       DAWAOG010000469         1750 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8910_length_1750_cov_1.830678, whole genome shotgun sequence.
ACCESSION   DAWAOG010000469 DAWAOG010000000
VERSION     DAWAOG010000469.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8910_length_1750_cov_1.830678"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    901..910
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          419 a          420 c          412 g          489 t
ORIGIN      
        1 cgaatacgcc gctatccccc gtgagatgat ggcaatagca ggctatttct caacagaata
       61 tctccgcgaa atatcacgtg ctgacgttat tctcaatatg agcattttaa ggcagaagtt
      121 cggcgatcgt gccgttctgc gcgcgctcca cttctttgag gaaaacgagc gtgtggacaa
      181 gcttgtgcat tcgctcaagc acggacattt cgatgatttc ttaagctcga taaacgaaag
      241 cggaaattcc agctacaagt acttacagaa tatatttgcc gtttccgatt atacccatca
      301 ggcagtcgga attgcactga acgttgcaga gcacgctctc acaagaaagg gcgcttgcag
      361 agttcacggc ggcggttttg cgggaactat acaggcgttt gttccgcagg atatcttaaa
      421 gagctttatc gttgatattg aaaaggtgtt cggcgcagga agctgtcatg ttctttcaat
      481 acgtccagtt ggcggtactg aggtggagct ttaattgtgc tgagggtgat attgtttata
      541 taagttgagc tttgcaaata acgatttgtt acattggaac attgtgacag cagcatatct
      601 gcttcacttt tcgtagggga ggggctcgcc cctcccgatg tatcgttata atcatagcga
      661 ataattgatt gtgtcagatt gaacgactga aatacctcga gaggggcaat gccttaagcg
      721 tgtgtgccca ccatacagac tcgcgatgac gatgataaat ggcttaatac ggcgttttta
      781 aaataaacgc cgcagacgta tccacaatta ttcattattc atcattcatt attcattccc
      841 taatggtgat gagtattccc cgataaaaca aaaaaacagc cccgcagggc tgttatcagg
      901 nnnnnnnnnn ttgtctgtta aataacaaat tcttcgtatt cgtgtttatg gagcttgtct
      961 acgagtgcgg ttaccgcaat accgtcctcg cgatactcct cgctcagcac ctttccgtgc
     1021 gagtggagtg ccgccgccag tcccgacttg tcatacggta tcagcatttc catacggctt
     1081 accttatcgg taaggttatc cgatatggct ttcagcaatt tgtcaaatcc cgtaccgttc
     1141 tttgcggaga tcattaccgt attttcggca ggcggcagtt cataatcgag cttgtcgcac
     1201 ttgttgagaa cagtgattac aggtatttcg ccgcagccga gctcagacag cagagaaagc
     1261 gttgtttctg ctttctcctt ggcttccggg tcggacacgt cacagacatg caggattata
     1321 tccgcgcttg ccgcttcttc aagcgttgac ttgaacgctt ctacaaggtg gtgaggaagc
     1381 cgtcttataa gtcctaccgt atcaacgagc gtaagacttc ttccgtccgg cagttcgatc
     1441 gcccttgatg taagatcaag agtagcgaac aatttatcct cggcaagcac accggcatcc
     1501 gtgagagcgt tgagcaaggt cgattttccc gcgttcgtat atcctacgat cgcacccacg
     1561 cacacgctgt ctttcttacg gcggcggcgt gacagatcac ggcgtttttc aagctcttta
     1621 agttcatcgc tgagtttatc tattctgcgt cttatgtgac ggcgatcggt ttcaagctgg
     1681 gtttcacccg gtcctctcgt tcctataccg ccgccaagac gtgacaatga cgtacctatt
     1741 cccataagac
//
LOCUS       DAWAOG010000470         1749 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8923_length_1749_cov_1.596222, whole genome shotgun sequence.
ACCESSION   DAWAOG010000470 DAWAOG010000000
VERSION     DAWAOG010000470.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8923_length_1749_cov_1.596222"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          531 a          374 c          366 g          478 t
ORIGIN      
        1 ggtctagggg cggtagcccc agtaatatag ccccttcccg aggggaaggg gtttggggat
       61 agggattgag aacaagcact tttttgaaaa aacatacttt ttctacaatc tgatacaacc
      121 tgcggaaaaa ttccgcaggt tgtattttta tgctttctat tgtaaaattc aatcaacaat
      181 ttattcagaa aagattcctt tcaataacct ttttctgttt ttcctgtagc aaatagttcc
      241 agacagtttc aaagaaattc ttttcttcct gtgtttcagc ttctgttata agctgtaaaa
      301 aatcatcgga atgcagattt ttaaaatctt catatacctt tgtgtatttt gaaacatcgc
      361 acatataatc gcctcatttc cttatattgc tgtaaaacaa atacatttag ccgttaactt
      421 attataacaa ctctactcca aaaagtcaaa cgttcaagta aattctatgt ttctgctgtt
      481 tgactatccc cctcacccgt gctataatta cagcagcaag aaaaatcacg gagggaaatg
      541 tatgcactat tatgaatacg gcaaagaaaa cgataagacg atcgtctttc tgcacggggc
      601 aaactttgtc cacagcttcg gcaggcaata tccgcttgcc gaaaaatatc atcttattat
      661 tccgcatatc atgggctacg gagatgccgc agatgagatt ttagataccg gaacagcagt
      721 aaaacagctt gcggatttca tagccgacat cggcaaaaag gtaacgcttg tcggcttttc
      781 gctcggcgct cagcttagcg ttaagctgtg tgcggaatat ccgcagtatt ttacttccgc
      841 gataatagta agtccgtggc ttatcaagaa agagcctatg ctctcaaagg tcatggctat
      901 gaacgaaaag cagtttgcgt cattcaaaaa caaacgatta tgcagtttca tcggatttat
      961 gaacggactg cccaaagagc aaagaaaaga atttgtcgca cagatgcaga atgtgcgtat
     1021 cgaaaccgta agaagctccg ttgacaacgg aataacgctt gaaacaataa agggctttga
     1081 aaacgcatac ttcccgatga tcgcccttgc aggcagtaag gaacagacag aagtacacga
     1141 cagcgtaaaa ggacttgccg ctatgaataa aaattgcaga tacgagatat gggacaaagc
     1201 cgcccataac attcctcccg tgttttccaa gcgatttaac gaacttatta taagaatggc
     1261 agaaatttga ctatgtacag aattttcatc attgaagacg accccacgat agcgcagacg
     1321 ctgaaaaagc accttgaggg ctgggactac aaggtagagt gtgcaaagga ttttcacaat
     1381 atcactgcgg agtttgccgc tttcgaccct cagcttgtac tgcttgatat aaagctaccg
     1441 ttctatgacg gctactactg gtgcggcgag ataagaaagc tgtccaaagt gcctgtcatc
     1501 ttcctctcct ccgcctccga taatatgaat gtagttatgg ctatgaatat gggtggagat
     1561 gattttatag caaagccgtt tgatctttcg gtgctgaccg caaagataaa cgcagtgctt
     1621 cgccgtgcct actccttttc gggcaacagc gaccttatat cccacggcag tgttatactt
     1681 gatatttcat cggcggtcgt gacaaacggc gataaaaagg cagagcttac aaagaacgag
     1741 cttcgcata
//
LOCUS       DAWAOG010000471         1747 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8930_length_1747_cov_1.807329, whole genome shotgun sequence.
ACCESSION   DAWAOG010000471 DAWAOG010000000
VERSION     DAWAOG010000471.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8930_length_1747_cov_1.807329"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    213..249
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    716..725
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          511 a          323 c          372 g          494 t
ORIGIN      
        1 cctgtgagat gatcggtatt tcccatatat cgtccttgtc aaaaccgcaa cagacgcggc
       61 atttggcaca cgcatcacgg ctgagtatct ttgtaagcat atcatcaatt cctttcactg
      121 ttatcttacc acaactttta cggataagca acaggtattt atattttggt cggtaataaa
      181 ttatgtcaat ttggttacaa tttgcaaaat ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnng ttttggtata attacttata tgtccggctg ttttacagac gcggataaac
      301 gattttacac ggaaacaccc gtagaagctt aaaggatata caatggataa taatatttca
      361 aacagttttg caaattacga atttatcccc gatttatttg aacagataga aaaggtaata
      421 ataggcaagc gagatgttat aaagatgctg atcgcagcac tgctttccgg cggtcatgtg
      481 cttatcgaag atgtaccggg tacaggtaag acaacacttg cgatggcact cgcaagagca
      541 acatctttgt catgcagaag agtgcagttc actcctgatg taatggcatc ggatataacc
      601 ggttttacta tgtacaataa ggaaagcgag cgttttgaat atcgtgaagg tcttgtaatg
      661 agtaatattt tcatcgccga tgagataaac agaacatcac caaaaacgca gtcggnnnnn
      721 nnnnncccga agctcagctt gaccgcttcc ttataaaact gtcggtaggc tatcctacag
      781 ccgaagacga ggctatgatc ctttatagaa gaaaaaacgg cgaccctata gaaacagtag
      841 agcctgtatg cagtgccgat gatatcaaaa gctgcattgc cgcagtaaga gaaacacata
      901 tagatgccgc aatttataaa tatattgtcg atattgttgc ggcaacaaga actcatccgt
      961 ttgcggctct gggcgcaagc ccccgtgcgt caatagcact tatgagagcg gcacagagta
     1021 cggcatttat ggacggacga aactatgtca ctcccgaaga tgttgcaatt atggtgaatt
     1081 atgttcttcc gcacaggatt catcttacac aggaggctaa ggtgaaacgt atcggggtgg
     1141 attatgttat agatgaaata atgaaggctg taaagccgcc gttcaaaggc aataaccagt
     1201 aaaccgaagg gacaactttt gtatggctag atataggttc ttttatttag tctttctgat
     1261 agtaagcata gtactgatgt tcgcttacaa aagcaaacta acatcggtat tgtttctgat
     1321 agccgttatt ttgccggcag tgtcatttgt tttacttctg ttgacgagat ttttactgaa
     1381 agtgcggata gaatacagga cgctttcgac agagaagttt gaaaataccg atatttccgt
     1441 aactgttaca aacagatttt tagttccggt ttcaccggga gtattgatcg gctgttttcc
     1501 gtaccgtaac agcgaaaagt ttgaatatca gaagataatg gtcagtgtac ctccgttttc
     1561 gtcaataacg gtcagcttta attccccgat aaagtacaga ggcgtatatc gctccggtat
     1621 tgacagcttt gtgatatatg atctttttaa agtattcaga tcggtgaaaa aatgcgaaca
     1681 atatgaaagg tttattgttc tgccgcgtaa gcttgttatc gatcctatta tcgatacagg
     1741 cgacggt
//
LOCUS       DAWAOG010000472         1744 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8955_length_1744_cov_2.792185, whole genome shotgun sequence.
ACCESSION   DAWAOG010000472 DAWAOG010000000
VERSION     DAWAOG010000472.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8955_length_1744_cov_2.792185"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          516 a          275 c          435 g          518 t
ORIGIN      
        1 tcctcagaca gtacgattaa ggttttgaac aagcttatcg aatttccttt aaaggcggct
       61 ataggcttac ttaccggagt tctggcatat cttgtataca aaaaaatcac ggaacacgac
      121 tacacaaagt tcggcaaatt tgttttgact gttatagagc tgttcggtta tttatttata
      181 cttatcgttt caattttcac aaggaaaata agcgatacat ttattatccc gctttttatc
      241 gcgacaacag caattacatt cagtgaaaaa agcgcactgc atggattgtt taaatggagg
      301 atcttcagtt ttctcggtaa agcttctctt ccgttgtact tcaatcatgg aactgtattc
      361 tatgctatca ttcggcttta tcccgacggc agttatcagc acaatatggc tatagcgata
      421 cctttgataa tactcctctc cgcatttgaa tacttcggag tcagagcaat tatgttgatg
      481 tggaaaaaat caggtataaa gcttattaaa aaggatcaag cagaaataac agaacagaat
      541 taaaattcgg atatactgtc gatttgaccg gttcaagtga acggcgggcg ggcggctgtg
      601 agccgcttca aatgcgccgt tctgaaggtt tgcgatttgt gcgacttccg ttaccggcgg
      661 gtggcaatgt gccgtggtaa attcgccgtt ctgactgttg ccgataggcg acattacgtt
      721 accggcgggc ggcacacttt aattttacat tgaaatttta tatcgaggtc cgttgtctga
      781 aaaggcgcgg actttttgtt taatgaataa tgaataatga atgatgaata attgtggtga
      841 ccgccgccgg cggtagattt ggattttggg tagtgcgggg cgagggatag tgcggggttg
      901 gaggtgatgg cagaaatggt ggaatgttgt agtagctttt gtgtgaagct aaacaaatgt
      961 acttgaagta gcatttaata accaaaactt cttgacaagt ttatagcaat aatgatatac
     1021 tagtgttatc aaaagaaaaa agatatttct gtaagaaatg taagggtgag agatatgtgt
     1081 aagacagtaa atgctaattt atgcaaaatt gggctaaata aaagcgcctg tgtaagttgt
     1141 gcacaatatc tctgtgtaaa tcaattcaat atggtaatta aggcatagtc cgcgaggggt
     1201 ggggtatgcc tttttatgtt ttaaaaggaa agaggtatgt gtttatgaaa ctgaaaaagt
     1261 ggctgctggg gctggtggct tttgcggcga tggtcgtgct atgcgtggtg tgcacgggtg
     1321 cggagactta tggggacctt gagtacagcg tgcttggtga cggtacggtg gagattactg
     1381 attataacgg tagcgcagaa aaggttgata ttcctgctga aattgacggg aagagcgtga
     1441 cgagtatagg cggttatgct ttcagtgatt gtgaaagtat tataaacata acgataccga
     1501 gcagtgttac aaagataggt tggtatactt tcaataattg tttaaagctt acaagtataa
     1561 agataccgaa caatgtgaca gagataggcg gttacgcttt cagcaattgc tcaaaactta
     1621 cgagtataac gatgccgaac aatgtgacag agataggttg gtatgctttt aaaaattgca
     1681 aaagccttac aagtataaag ataccgagca gcgttataag tataggcgat ccagcttttt
     1741 atgg
//
LOCUS       DAWAOG010000473         1744 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8959_length_1744_cov_2.100059, whole genome shotgun sequence.
ACCESSION   DAWAOG010000473 DAWAOG010000000
VERSION     DAWAOG010000473.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8959_length_1744_cov_2.100059"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          503 a          397 c          398 g          446 t
ORIGIN      
        1 tgttcaaaag catacaaatc agtacaagga gagatttaaa atgaaaaatt ttaaaagagt
       61 tcttgcaatg gcaactgccg caactctggc agtaacagca tttgcaggtt gttcatcaag
      121 caacgcaaac agcggtaatg ttagcggctc aacaagcgca gcagatggcg aaaagacaat
      181 cgttatcggt ggcagcggtc ctcttacagg tgacgcggct cagtacggcg taggcgttaa
      241 gaacgctatc cagcttgctg ttgacgaagt aaacgcagca ggtggcgtaa acggcaacaa
      301 gttaaagctc gtatttgaag atgacgaggc tgactcgggt gataaggcag ttaacgctta
      361 caacacgctg aaggatcagg gtatgcagat attcctcggc acagttacaa caggttcttg
      421 ccttgctgta gttgacaagt ctaaggaaga taacatattc cagttcactc cttccgcttc
      481 cgcacaggat gtaatcgcaa acgataactg cttccagatc tgcttcacag accccaacca
      541 gggtaaggca tcagctgact atatagctga caacaaggta gctacaaagg tcggcgttat
      601 ctacgacagc tcagatgctt attcttcggg catctataac gcattcaaga cagaggcagc
      661 agcaaagggt cttgagctcg tttcagagca gtcgttcaca aaggacagca agacagactt
      721 ctcagctcag atagctgact gcaaaaacaa gggcgctgaa ctcgtattcc tgcccatcta
      781 ctatcagcag gcttctctta tcctcacaca gagcaagaac gcaggcttct cacccaagtt
      841 cttcggttgt gacggtcttg acggtcttac atctatcaag aactttgata cttcacttgc
      901 tgaaggcgtt atgctcttaa cacctttcgc agctgactca aaggatcagg ctactcagga
      961 cttcgttaag gcttataaag cagcttacaa cgatgaaaca cccaaccagt ttgcagctga
     1021 tgcttatgac ggcgttaaga tccttgcaaa gctcatagag agccagggca ttacagccga
     1081 tatgagcgat ggtcttacag gtacaggcat gaagtggaac gctaacggcg aagtttccaa
     1141 gtcacctaag gctgttgtta tcaaggatgg taattactca gctctctaag ccgataaaac
     1201 cccgaaagct taattacgga aaaaacccga caggctattg tctgtcgggt ttttgaatta
     1261 tctgcaaaaa aatgttgcaa acggctgtgt gctgtgatat aataaatagt attagaactt
     1321 cactaaggaa ggatgtatac atatgggttt tctcaataac ctcataaacg gcataagcct
     1381 tggcagtata tatgccgtaa tagcactcgg ctatacactg gtatacggca tagccaaaat
     1441 gctgaacttc gctcacggcg atgtaatcat ggtcggagga tatgtaatat tctattcgac
     1501 tacttcattc ggactaaatc cctatctctc ggtactcata gcggtagttg cgtgtactgt
     1561 tctcggtatc gtgattgaga aggtcgctta caagccttta cgacaggcaa catcactctc
     1621 ggtactgata actgcaatag gtgtcagtta tttcctgcaa aattcggcgc ttttgctgtt
     1681 cggcgagaaa cccgttaact tcacctctgt tgttaatgtt ccgagcattt cactctttga
     1741 cgga
//
LOCUS       DAWAOG010000474         1742 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_8972_length_1742_cov_2.189686, whole genome shotgun sequence.
ACCESSION   DAWAOG010000474 DAWAOG010000000
VERSION     DAWAOG010000474.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8972_length_1742_cov_2.189686"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    377..386
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    811..885
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          513 a          365 c          438 g          341 t
ORIGIN      
        1 gtataccgat gagtttaaaa tacttctttt accgaaagac cccgatgatg acagaaatgt
       61 cattattgag ataagaggcg gtgccggcgg agaggaagcg gcattatttg caaactcaat
      121 gttcagaatg tactcaatgt acgcgcaggc taagggctgg aaaacagaag tgataagctc
      181 aaacccgaca gagcttggcg gatataaaga gataagcttc tcggtagagg gtgagggcgt
      241 atacgctaaa ctcaaatatg aaagcggagt tcatcgcgtt caacgtgtac cggaaacaga
      301 agctcagggc agaatacaga catcaaccat aactgtcgcg gtccttccgg aagtcgagga
      361 ggtcgatgtt gagatannnn nnnnnnagat aaatcctgcc gaccttgaat ttcagaccta
      421 ccgttcaagc ggtgcaggcg gtcagcatat caataagaca gaatcggcta tacgaataat
      481 ccaccaccct acaggagtag ttgtagagtg tcaggaggag cgaagccagt ataagaacaa
      541 ggacaaagct atgaagatgc tgtacagcaa gctgtatgag gcaaagctga acgagcagaa
      601 cagcaagatt gcggctgaac gtaagataca ggtcggcaca ggcgaccgtt cagagcgaat
      661 aagaacctac aactatcctc agagccgtgt tacagatcac aggataggac tcacgctgta
      721 caagctggag cagtttatga acggcgactg cgacgaggtg tttgacgcac tggctattgc
      781 cgacagatcg gcaaagcttg ccgcaggcgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnataag gtgacctttt
      901 tgaaaacaga gataaagaag ccgtcttacg aggtattatg cgaggcggca gagcttataa
      961 agaagggtga ggtcgtggcg ataccgaccg aaacggtata cgggcttgcg gcagacgcat
     1021 ataaccctga agcgtgcgcg aagatattca aagcgaaagg cagaccgcag gacaatccgc
     1081 tgattatcca catctgcgac cttgatatgc tgaaaaattc ggtaaaggag attcccccgc
     1141 ttgcgcttac ccttgccgag catttctgga gcggttcgct gacgatgatc ttcccgaaga
     1201 aagatatagt tcccgatacg acaagcggtg gacttgatac cgttgcggta cgaatgcccg
     1261 acaacgagga tacgcttaat cttataaaga tgtgcggtgt acctcttgcc gcaccgtccg
     1321 caaatctgtc gggcagtccc agtccgacaa cagcacagca cgtctatgcc gatatgaacg
     1381 gcagaatacc gctgataata gacggcggcg aatgcaaaaa cggtgtcgaa agcacagtta
     1441 tctgctttac cgatggcgga acagggataa aggtgttaag acccggtgtt attaccgctg
     1501 agatgctcag ccgttttgca aagacagaaa ccgacaagaa tgtatttgca aaacccgacg
     1561 aaaacgaaaa ggtaatttca cccggcgtta agtacaagca ttattcgcct aaagctgaca
     1621 taactattgt agaaacggat agcgatgaga aactcgccga gtttctgaat gataaaaatg
     1681 acggcagcac ttacggtgtt attttcggta acgaaacagg cattgacata ccgaccatat
     1741 cc
//
LOCUS       DAWAOG010000475         1738 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9013_length_1738_cov_1.999406, whole genome shotgun sequence.
ACCESSION   DAWAOG010000475 DAWAOG010000000
VERSION     DAWAOG010000475.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9013_length_1738_cov_1.999406"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          384 a          427 c          379 g          548 t
ORIGIN      
        1 ttcgactcat tttttcgggt tgtgacacaa ccccattttc cttataaaag caaaaaggac
       61 tgcgcaaacg ctacagtcct cttttttaca ttacttgtca agattgtact tcttcttgaa
      121 cttgtcaaca cgtccgcctg tgtcaaccaa cttctgcttt cctgtaaaga aagggtggca
      181 cttcgagcag atttcaacct ttatatcctg cttggttgat cttgtttcga taacctcgcc
      241 gcaagcacac ttgatagtgg tgggctggta gttagggtgg ataccttctt tcatgctgtc
      301 acctcctgac cgttataatt gatctaaatt acagctttcg ccatgatttc agaattgtgc
      361 gctttcgcgc ttaggttatt attataacac agtcaaatac attttgcaat agttttttta
      421 aattttcctg aaacttgtgt attagttacc tgttcagcct actatctttg tgaaatctgc
      481 cgagagcttg acctgctttt cagccgcatc ttcaacgctt gtgcctaccg ttgtgtagta
      541 gagcttgatc ttgggctcag taccggaggg tcttattacc acgcttgcat tatcctcaag
      601 gtagaatgtg ataacatttg acttaggaag tgtaagctct tctgtcttgc cgctttcgta
      661 atctgtcttt acgctgagct tgtagtcagc cttgccgata accttaagac ctgcgacagc
      721 ttcaagcttc tcgtttctca gtgtatccat gatggcgttc atcttcttca tacctgtttc
      781 gccctcgaat gtgaacgtat caagcttgtt gcagtagaag ccgtagtcgc tgtacatctt
      841 tgctcttgcc tgtacaagag aaataccctg tgaacgatag aatgccgcca tctcgcagat
      901 aagcatagat gccacaacag cgtccttatc tcttacatat gagcctgcaa ggtagccgta
      961 gctctcttca aagccgaaga tatatctgtt ttcctcgccc tttgcctcta agaagccgat
     1021 ctgctcaccg atgaacttga agcctgtgag gacctctctg agctcaacgc cgtacttagc
     1081 ggcgatagcc tttacaaggt ctgttgtaac tattgtcttt accgcaacgg ggtttgcagg
     1141 cattgtacct gccgcgatac gctccttgca gatatattcg agcagcattg cgcctacttc
     1201 gttacctgtg aacagaacat acttgtcacc gtcgggaact gcgataccta cacggtcgct
     1261 gtcggggtcg gtagcaagca gtaggtcggg cttttctgtc ttgcaaagct caagacctaa
     1321 gtgtaatgct tcctttatct cggggttggg ataggggcag gtcgtgaagt tgccgtcggg
     1381 catttcctgt tccttaacga ctacaacgtt gcttatgccg atttccttta agatgcagcg
     1441 aacgggcttg ttgcctgcac cgttaagagg agtataaacg accttcagtc cgctctttgc
     1501 gcaaagctcg gggtggatag actgcttctt gacgtttgcg atatattcat cgataacgtc
     1561 ctgaccgatg tactttatct tgccttcgtt tacatactgc tcaaagtcac cgctctttac
     1621 atctgcgaag atattgagct tctcgatctc gccgagaatt ttttcagcgg cttcaagcgt
     1681 aacctgacag ccgtcacttc cgtaaacctt gtagccgttg tacttagcgg ggttgtgg
//
LOCUS       DAWAOG010000476         1737 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9022_length_1737_cov_2.073722, whole genome shotgun sequence.
ACCESSION   DAWAOG010000476 DAWAOG010000000
VERSION     DAWAOG010000476.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9022_length_1737_cov_2.073722"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          520 a          373 c          414 g          430 t
ORIGIN      
        1 gttacatcac cctacacact gtacagcgaa gattttgctt cattcggtga ggacgatgtt
       61 tacgatcaga cagactcaaa gggctttatc aacctgttcg gtctgccgat aaaggtcaag
      121 gctctgcttg acgctgagag aaacaataag taagctttaa aagcgttacg gtaaatcccg
      181 caaggtgtaa aactatgcct tgcggctttt acggttttat aagatacgat tcgtaccgac
      241 ttttcggtcg gtacggtatt gtgttttatt tacactttat atttataacg gaaaaaacaa
      301 gacgcttttt gcgttatgaa cataaaagca taaatctgaa aggaaacaac agtatgtcta
      361 aaatgatgtg ggcaggacga ttttcaaagg aagtcgacac aaaggtcaac gactttaatt
      421 cttctatctc atttgacgca agaatgtaca agcatgatat aaagggctcg atagcacacg
      481 ctacaatgct aggtgagtgc ggaatcatcg accccgccga cagcaaggtg ataatagaag
      541 gtcttaacgg aattttaagc gatatcgaaa gcggaaagct tcatttcgat ccaaatgcag
      601 aagatatcca tatgttcgtt gaggcggagc ttaccgcccg tatcggcgat gcgggaaagc
      661 gtcttcacac cgcaaggagc agaaacgatc aggtagctct tgatataaag ctttatctta
      721 aggacgaagt cgatgagatc gcaaagcttg tccgcgagct tataaatacg atcatcgata
      781 tcgcaaagca gcacaccgaa acagtaatgc cgggctatac ccacttacag cgtgcacagc
      841 ctgttacatt cgcacatcat cttatggctt atgcccagat gctgacaagg gatctgacaa
      901 ggcttgcaga ctgcaaggaa agaatggacg taatgccgct cggttcgtgt gcgcttgcag
      961 gcacgacata ccctcttaac agaaagagaa ccgccgagct tctcggtttc aagaatatta
     1021 cgatgaacag ccttgacggc gtatccgaca gggatttctg cattgagctg tgctccgaca
     1081 tttcgatgct tatgatgcac ctttcaagat tttctgagga aataattatg tggtgctcat
     1141 gggagttcaa gttcattgag cttgacgatg cttattctac aggcagctcg ataatgcccc
     1201 agaagaaaaa tcccgatgtt acagagctta taagaggtaa gaccgcgaga gtatacggtg
     1261 atctgcagac gcttctcaca atgatgaagg gacttccgct tgcatacaac aaggatatgc
     1321 aggaggacaa ggaggcaata tttgacgcag tggatactgt aaagctgtgt atctcgacat
     1381 tcactccgat gcttgcgact atgacagtac ttaaagacaa tatgcgtact gccgccgcaa
     1441 agggctttat caacgctaca gactgcgcag actatctcgt taagaaaggt ctgcctttca
     1501 gaacggcata caagataaca ggtactctcg ttgctctttg cattgagaag aacacaacgc
     1561 ttgaacagct cccgatagaa gaatataaga agctgtgtga aacatttgac acagatgtat
     1621 atgacgctat cagccttgat acctgcgtta tgcagagagg cgttgacggc ggacctgctc
     1681 ccgatgccgt aaagcgtcag atagcagagc ttgaaggcag actgggcggt atcaata
//
LOCUS       DAWAOG010000477         1737 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9021_length_1737_cov_2.679548, whole genome shotgun sequence.
ACCESSION   DAWAOG010000477 DAWAOG010000000
VERSION     DAWAOG010000477.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9021_length_1737_cov_2.679548"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          418 a          400 c          380 g          539 t
ORIGIN      
        1 gtgtataata tgtgcgccgt aatcagcgaa tgctgcacct gtcttgtgag ccattgagtg
       61 aacgataccg agcagagcat ttgagaatgc catacctgcg aggcactgtg cgttgtgcat
      121 tgcgtcacgc ttagccatat cgccgttgta agaatcaacg aggtcgttct ggatcatctt
      181 gattgcgtga agtgcaagag gatctgtgaa gtcgcagtta gctgttgaaa cgtatgcttc
      241 gatagcgtgt gtcatagcgt ccatacctgt atgtgctaca agcttctgag gcattgtttc
      301 tgcaagctcg ggatctacga tagcaacatc gggagtgatc tcaaagtcag cgataggata
      361 cttgataccc ttgtggtaat ctgtgatgat cgagaatgcg gtaacttctg tagctgtacc
      421 tgatgtagaa gaaatagcgc agaagtgagc tttctttctg agctcgggaa taccgaatac
      481 cttacacata tcttcaaatg tgcagtcggg gtactcatac tttatccaca tagccttagc
      541 tgcgtcgata ggagaaccgc cgcccattgc tacgatccag tcgggctgga acttgagcat
      601 tgcgtctgca cccttcataa ctgtttcaac agaaggatcg ggttctacat tctcaaagag
      661 ctcgacctcc atgcctgctt ccttaagata agcctcagcc ttctgtaaga agccgaaacg
      721 cttcatcgaa ccgccgccaa cgacgatcat tgccttcttg cccttaaagc tctttaatgc
      781 ttcaagtgcg cccttgccgt gataaacatc tcttggtaat gtaaatctca tatgaaattc
      841 ctcctgttcg gcttatagcc gttaataatc tatatttagt tgttgttatc gtgcgccctg
      901 cgttatgtat ttgtgttgca ggacttcttt cttttgttcg ttattatttt aacacactga
      961 gtcggaattg taaaatatat tttaggaata tcggtatgta attatctata taacgatatc
     1021 gatttaaatc tgcgaaaata cggtttatat cggcattcgc cgcagcaaag ccgtttgtga
     1081 gctttatgta acagatttgt gaaaagtgcg taaaagacta aatgttaata tctgtcttat
     1141 tttttagtgg ataaatattg aattattggt aatgcaaaat cggacacaaa cgcttttccc
     1201 gatgggtagg aaaagtgtct gtgtccgact gagatcggct tatatcaaat tttatccgtt
     1261 cacatattcc ttgggtgata tccacttgcc gctttgcttt acagcaaaat gaagatgcgg
     1321 tgcaagcttc gactcgcatt cgatagtccc tgtaacgcct atcttctgcc cggactcgac
     1381 ctcactgccc tctttcacct caagcgattt gtcaagtccg taataatgcg tttcatatcc
     1441 gtcaccgtgg tcgataataa cgcatactcc ccatagcgga tcgtccttta tctctttgac
     1501 cttgccagtg caggcggctt ttatgtcagt tccctgtgcc gcggcaatat ccataccatc
     1561 gtgggttttc cacacgccga gcgtttcgct ctttacaagc tcgccgttgc tgtattccca
     1621 tattacctcg ccatcgatcg gcattacacg ctttgcggtg cttctgacaa agttatttgc
     1681 aggtgcaacg gaactgttgt cgttactctt tgaggtttca tccttaggta ttccgct
//
LOCUS       DAWAOG010000478         1734 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9051_length_1734_cov_1.693865, whole genome shotgun sequence.
ACCESSION   DAWAOG010000478 DAWAOG010000000
VERSION     DAWAOG010000478.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9051_length_1734_cov_1.693865"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          454 a          426 c          310 g          544 t
ORIGIN      
        1 aagaatgccg ccgctatcac aacaagccat gcgaacggtc ttaaaatgct gaaatagatg
       61 aatataacgc ttacggcaaa acagcaatac gatatcagtc ttaatatctt tacattaccc
      121 gaagtgaaca cttttccctg tctgatatta gacagcagac ggtcaagcgc tataactatc
      181 acaagcgcag ctgccgccgc aagataaaga gtgattatcg taggcagata gaccgacatt
      241 ccgaacagtg caaatatgcg gttatcgtag atacggacaa taaaaggtgc aaatatacag
      301 catacgatta tcagtacgaa taacgccttt acaagtattg aggttatcat tacggaatag
      361 ttttgttttt tcataatacc gccctttccc gacgtagttt tttgttgttt gaattatagc
      421 acagggtttt ccgtttgtca atagattttt attgtttttc gataattttt tattgctgac
      481 taataaaacg caggagctgt gacgaaatta ttgcatttcg tcacagctcc tgctttaggt
      541 attatttgca ccttttcaat gctcttatta tccttatata ctccttgccg acagtgacca
      601 gaagcttttg cgcactgtca taatcgcctt ttcttaaata ttccgttatt tcgctgaaca
      661 cactgcggaa cttttcaaat ccaagatttg cggctattcc cttcatggtg tgcgccgctc
      721 tgaaagcctt ttcgaccgaa tgctttttca ttgcatcagc aaactccgca aagtgcttat
      781 caaacaaaaa ttcgcgtatt gcaggttcaa cattctcttc ttcgcactca agacgctgta
      841 gcacgctgtc atagtcctct cccaacaagt catagcactc tctcattgtg agattttttc
      901 tgccgatata gctgaacacc gcatccttta atttttttga ctcgacaggt ttagcaagat
      961 gctcattcat acctgccgtc tgagtcttta tcttatcgtc ggtaaatgcg ttagccgtaa
     1021 gcgcaattat cgggacagat attgcatctg cgcgataaag atgccgtatc gtttcagaag
     1081 cttcaagccc atccatgaca ggcatcataa tatccatcag aattacatcg atcgaataaa
     1141 gtacagagct ttcaaatatt tcaacggctt ctgcaccgtt ccacgccttg atgaccgttg
     1201 caccctcact tttcagcata aattctgtga tctccatatt cagttcgtta tcttctgcca
     1261 gaagaatatt cacacctgca agtgagcgat cctctaccac gaccccaggc tttgatttgg
     1321 accgcctcgt catatctata tccaacggaa gaactatcgt aaaatcagta ccgacatttt
     1381 tcgtgctttc gcactcgatc gtgccgccca tcttttctgt aagccgttta gctatcggaa
     1441 gccccagacc tgttccctga tatgatgttc tggcgctttc attttcctgg cggaacggct
     1501 catacatcac cttccggaaa tcttcaccta tgcccttgcc gttatcctcg cattttatac
     1561 tgagcattgc tgtttttccg tcctcggtaa cagaaagctc tttgcaggat acttttacat
     1621 atccgccctc tcttccgtat ttaacggcat tgctgattat attcataagt acccgcttaa
     1681 catgaagcgg actgccgatc agcctgttat gtatattttc gcacttttca aaag
//
LOCUS       DAWAOG010000479         1728 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9098_length_1728_cov_1.842200, whole genome shotgun sequence.
ACCESSION   DAWAOG010000479 DAWAOG010000000
VERSION     DAWAOG010000479.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9098_length_1728_cov_1.842200"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1142..1151
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          491 a          408 c          302 g          517 t
ORIGIN      
        1 aaagcatcgg tgtatccttt ggcaaataaa agcaacgcct tcacttattt atggtgtcag
       61 ttatcgtcag gattgcgaca cagcaaaatc aagcgataac atagttctgt ataatcgctt
      121 aacagattta atcatattgc agaaatgccc ccaacataaa gcttgggggc atttagtata
      181 taattttcac tctgtcataa acagcttagc cgcaccgccg aatatttccc gggaaagctc
      241 ggcgctcata tcgggattat ccgcctttat cctctcacag cgctcaatat aaccgacaat
      301 atcgacattg caaagcttct tttcgctatc accggaaagt attttgtcaa cagcctttga
      361 tgcttcttta taaaaatcgc tctcaagcat acgctcagct acatcggcat ttttatcgcc
      421 gcaaacaggc atatagctgc tttcataatc aatcacaaag cagtcggtat actttatgaa
      481 taactcttcg caatacgaag taagacggtt cttttcttcg atatactctg tatcctcaaa
      541 cggtcttatt cgtcccgtca tatcaagacg gttaagcgaa agacgcgtct ttctaaggat
      601 aatcttgctg ccatatcttt cggttaccgc tttacaaaag ccttttacgg catttttaat
      661 atattccttg tcaaaatcgt cggcatacgg agcgaaactc tcgtcatcgg tcataaacgc
      721 tctgtacacc attgtatcat ataagccgtc aatcgccgca atatatgtat tattgtgact
      781 gtaaagctct ttttccgcat cggaaaaatc aacgatcagc caatctgcat tcttgtccgg
      841 gaaatatgaa cgtgtaagtt ctctgaacac atcagatgta ctgtcaacaa agctgagatc
      901 tacatcggct ttctgttcac ataccgtaag cggggaaaag ccgcagatac agttatttac
      961 aacagcttta tcgcacagcg aaagtaaagt tttgctatcc gttctgccaa acgtatcaaa
     1021 cgcaaacggt ctgttctttt tgtaatattc ggatacaagc tgctccacag cactcttgtc
     1081 tgcaccggac agccacaatc ttgcacactt gagatagaac tctatgctgc acttatcaag
     1141 cnnnnnnnnn ncgcaatccg gtatgatgcc ttttttacgg aaaaacgccg ccaacatatc
     1201 tgccagatcg tcttttgcat aaagaccgag ccttataact tcaccgatat cagggtcagc
     1261 gacttttcct ttgcgtatag cgcacactgc ttcaaacact tttttaagcg taacgttttt
     1321 tccaaagtca tatcgcaatc ttatctcttc aaacgttata aagttgcttt ccttgagtct
     1381 gagtatatcg ttaaattccg cacagataaa atcactgctc aggcgactta tgaaataatc
     1441 gacagcatta ttatgtgcaa agaaaaattt atcacagccg cttgatttta tagcatcgat
     1501 attttctgct atttccgtaa gccatatttc acagtcatat gacaatgcgt ttatgtaact
     1561 gcccttttcg cccgaaccga ttttttttgc aacaagtgaa atattgttgc ggaacaactc
     1621 atcgcggcac acggcatagc ttctgccacg cttattgata actccgggat aatagcggga
     1681 tatgtcaatt atcttaggcg atacacggcg tatcagatag ttctccat
//
LOCUS       DAWAOG010000480         1722 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9136_length_1722_cov_1.862028, whole genome shotgun sequence.
ACCESSION   DAWAOG010000480 DAWAOG010000000
VERSION     DAWAOG010000480.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9136_length_1722_cov_1.862028"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1484..1493
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          408 a          447 c          329 g          528 t
ORIGIN      
        1 ttagtcccat tttagtacag atatctcagg atagtagcgt tcttttttga tagtcagtga
       61 cagtgcagtt gcaccgattt actcacaaaa ttattaactc cttatcggca cggaaattgt
      121 tttcagcggt gttggctgaa tatattgttc tgactgtttt gcaatatgtt gcattgcaat
      181 tttaatgtaa tttgataaac tgaagaattg tgccacaacc ccttgcatat caattaaagc
      241 ttactgcata tcctgcgata atattttcct cgcctcgtcc tttaccctgt ctttgagcca
      301 ccccggcgaa acaacctcgc aatgacttcc gaaccccatc acaaagctca ttatatcttc
      361 ctcgttctgc attgtgcacc tgagctcggt cgtgtcttca tcaataagtg tcactgtctg
      421 atctctgccg taaatgcgtt cacgggcaag cattgcgtat ctgccgtgca aacgaagtgt
      481 tatctcatgc agttcgccgt tctttttcat accgaactca tcaagatagt cgctttcctt
      541 atatgtcgca agaatacgga atttatcctc ggtgactgtc attgactgaa tacggctgag
      601 cttgaagtac cgtatttcat acgacttctt atcataccct agcataaacc atgcattgtt
      661 atacataaag agcttgtatg ggtcaaagcg gcggtgcgac accttatttg acagcgacag
      721 atagtccatc tcaagctctt ttctgtgagc tatgcacccc gccacgactg agtaccttct
      781 gccaagctcc cgctcgtcca cagcaagcgg aaaacggttt gatacaaata tatcctgcgg
      841 cgttccgtgc ttcactgcgg caaaaacctt gccgatcgct ttttcatatt cactgccgta
      901 tggaaaatca ttacgacact tgagatattc agacccggac gacagggcat tctgctcctc
      961 gtcagtcagc tttaccgagg ggataagccg gtctgctatc agatgatacc caccgtattt
     1021 tcccggtatg ccctctataa aatatcctgc ctgttcaagc tctgtgcggt actccgctat
     1081 attcctgaca tttgtttcaa ggatatctgc aagctcctga cctttatata ctctgcccga
     1141 gcttaaaagc tgaagcattt tcagacaatt tgccgtttta cccatgtatc ttcctccttg
     1201 tacaacagaa taatacaagg taattataca ttatttatgt tacaaatgca attatcagag
     1261 gcgagctaaa aactcctttt tcttctctgc aaactcttct tctgtaagta ttccgttttc
     1321 tttcatctca tggagctgtt ttatctgctc catcacagcc tgaggatctt cctttttagc
     1381 cgcgggagct cggttctgaa tattctgtgc cgcctgcgag gctttatcct tcaggctctc
     1441 tgccgcaccg gcaattccgc ctgctgcatt ctgtgccata tttnnnnnnn nnntttccgc
     1501 gcctgtattt gtcggcaatg cggcggtatc tgcttcggca gaatttaccg cgtcaaagac
     1561 cttgttgccc ttaagaccta agaactgtat aacggacatc gcgataagtg caagcataag
     1621 tataagtatt atccagaagc ctatctgata gctaagctcg acctttgccg aaactcccgt
     1681 acctgccgca gctccgcctg ccgccgcatt tacagcgcct gc
//
LOCUS       DAWAOG010000481         1719 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9158_length_1719_cov_2.206731, whole genome shotgun sequence.
ACCESSION   DAWAOG010000481 DAWAOG010000000
VERSION     DAWAOG010000481.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9158_length_1719_cov_2.206731"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    601..610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          507 a          403 c          319 g          480 t
ORIGIN      
        1 attctatcaa gtataagcac tcctgtcttt ttgtcaagct cgtactttga cttacttccc
       61 tttgagatct caatgacagc taaaaagtca ttgggagtta ctctttcttt gtcgatatcg
      121 tgccagatgt tcataaaatt aacctttctt agcattaacg ctactaatgt atctattata
      181 caactatatt aacaattttg caagcgattt ttgcattttt ttgaaatatt tttcaaaaaa
      241 aactaaaatg ccgaaaattg aaatatctaa acgcaatatt acgctttctc aaacgccctg
      301 agcgtcttta tagcaatagc cgcaacaaga agtccgcttg ccgtccatat aatcggttcg
      361 gatatacata cgccgaaata accgcatacc gggataaccg caactacagt cactatcttc
      421 catatcatct cgacaatgct tgctcctatc ggaacaacgc tcttaccgag cccctgcaat
      481 gtgcatctca gcgacagaag cgtattcagc gcaaagtaga acggcacatt tatcctcaga
      541 tagttgaccg ccgtatctat aatttcacgg ttatccgcag gcgctatcat aagcacaagc
      601 nnnnnnnnnn cacaagctgt tctccgaaca gatacacaat aagcaccgca agagttgacc
      661 agccgtagcc tatcagcaga ccgtatttta tgctcttctt tattctgtca agccgacctg
      721 cgccgagatt ttggctaacg aatgtaacaa gcgttgcgct gatcgccgag aacggcatga
      781 tagtaaagct gaatatcttt cgtgcagcag tgtgagccga gattatttct gttccgagtc
      841 cgttgatgcc gttttgcaga actatcgagc ctatgtcaac tatcgaatac ataagtgcca
      901 taccaagacc tgaagcaata aggcttttta tctcatcgcc gtgaagcctg aaatcggact
      961 tgcccagctt taaaaacgga cattttctga taagataaat gaaacagaca atagcggaga
     1021 taagctgtgc gataactgtt gcaagcgccg caccgtcaac ccccataccg aaaccgaata
     1081 tgaacagaaa atccagtccg acattcagca cagctgaaat tacaagtatt atcagcggta
     1141 ctatgctatc gcccaaagca cgaagaatgt ttgcttcaag gttatatata agcgtaacta
     1201 taagtccgcc tgccactatc gtaagataca gcttcgcctg ttcaaaaatc tcagccggcg
     1261 tattgagtgc agtaagcagc ggctcaacaa acactataca cagcgtcatg attacagcgg
     1321 taattattgc agaatatgta atcattcctg caaccgtgcg ccgcaccttg ttcatatcgt
     1381 tagcaccgta gtgtttagca acaggtatgg caaaacctgt gcataagccg ttaataatgt
     1441 tgaacataag cgacaccacc gccgttgtcg aacctacagc ggacagcgat aattcaccga
     1501 gtttttttcc gacaattata atgtcgacca ggttatacgc ctgttggaac agactgccga
     1561 gcagaatagg caggctgaac aggagcataa gccgtattat gcttcctttt gttagatctc
     1621 tcatagtttc ttcttttctt tccgttgttc ttttactttt cttttagcta aaaacgacat
     1681 ttttaaggta atcgttttcg atgaaatata gtataacat
//
LOCUS       DAWAOG010000482         1719 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9159_length_1719_cov_2.128606, whole genome shotgun sequence.
ACCESSION   DAWAOG010000482 DAWAOG010000000
VERSION     DAWAOG010000482.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9159_length_1719_cov_2.128606"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1317..1326
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          470 a          408 c          329 g          502 t
ORIGIN      
        1 cttttaccct ttccgtttat aaataattat ttttaacgaa agtcaacaga ctatccttat
       61 cgtttttgca atttccgtct atcacttgct caagcagaaa gctcagagct tcaccgattt
      121 tcctgccgga tataccaagc tgcgaaatgt ccgagccatt gaccttaagc tgtttaatat
      181 ccataggcgg ttcattttca aggtactctc ttgtatgttt ttctatgctg tcaaacagag
      241 ctttttcacc gaaatactca ggagcttttc cgcaggtgtc aaaataatgc actctgataa
      301 gctttaaaaa gcgctccgcg ccgtatcgtg caagaccttt tcttatactg cggatatcat
      361 cgttaataac tataccatga ttttgaatta aaaacaatac ctcatcggta atttttttac
      421 tgaaacgcag tctttcggta actccggcag ctatcttaca gctgacatct gcatgaccgt
      481 agaaatgccc tacgccgtcc ctcataaaaa agctggcagg ctttccgata tcatgcagga
      541 gcatcgtaag ccttgcttca actgtaggtt cggccgcctc aacggttgca acaatatgac
      601 cgtataccgt cttatcgtga tattttgagt gctgctcaaa gccgatacaa ggatacatct
      661 ccggaatgat ctcacagata acatcgctgt aattataaag aatatcaccg caatcacctg
      721 taagtatccc gcaaagctcg tagtttatcc gttcagctga tataaatgac aacagataac
      781 gcaatctgtg aactgcgtca gaggtacatt tgtcgatatt aaaaccgtat cgcgatgcaa
      841 atctaagtgc gcgtaatatg cgcagtccat cctcatcaaa acgtttcatt ggatcgccta
      901 cgcttcttac aatacccgat ttcagatcct cctgaccgcc aaacaagtca atgatgtccg
      961 tttcaggact aaaagccata gcatttattg tcagatcacg acgtgccaag tcttcctcaa
     1021 gacaaggagt aaaacgtact tctgacgggt gacggtgatc cttataatca ccgtcaactc
     1081 tgtacgttgt tatctcaaca ggcttcttgt cgattataac cgtcacagta ccgtgcttaa
     1141 gtcccatgat caacagctta tagccgtcga atacctttat tatctgttcg ggcaatgctg
     1201 atgttgtaat atcataatca tcggggcatt gcccgagcag ataatctctt acacagccgc
     1261 cgacacaata cgcttcgtaa ccgctgttat gcagtttttc aagtacttca taaacannnn
     1321 nnnnnngata tcgtcttata taagatatta taatctcaaa ttattcgttg atagctgtaa
     1381 ctacgcctga accaactgtt ctgccgcctt cacggatagc aaaacggaga ccttcttcga
     1441 tagcgatagg agtgataagc tcaacatcca ttgttacgtt atcgccgggc atgcacattt
     1501 ccttgtcagc gggaagtgtg attacgcctg taacgtcagt tgttctgaag aagaactgag
     1561 gtctgtagtt tgagaagaaa ggagtatgac gtccgccttc ttctttcttt aatacgtaaa
     1621 cctgaccctt gaacttcttg tgggggtgga ttgaaccggg cttgcagaga acctgaccac
     1681 gctcgatttc gcttctctgg ataccacgga gaagtgtac
//
LOCUS       DAWAOG010000483         1718 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9163_length_1718_cov_2.145520, whole genome shotgun sequence.
ACCESSION   DAWAOG010000483 DAWAOG010000000
VERSION     DAWAOG010000483.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9163_length_1718_cov_2.145520"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          425 a          385 c          401 g          507 t
ORIGIN      
        1 tattgatatt gcctttcaga ccgataagca aggtatttga caaagaatac gcaggcgaag
       61 ctccgcatct gttgcttatc atatttttcg cagtattcta cggcttactt accgcattgc
      121 gttttatccc aaagctcaga gagatgaaaa cagactgtgc cgcattgctt atatcgaccg
      181 cacagtttac agtcgcttat gctgtagtca tgttccgtga aaaaggtaga agcattgatt
      241 tttttctcgg tgttgtcctg tttatagccc tcgctgtatg gtatactgta ggaaagcata
      301 ggatcaggct tccgaaattc acaaagaaat gctggatagc ggttattgcg ttgtccgcca
      361 tttttatgct tgtgttcacc gcacttgcaa tggcaatgcg gtattacaag ctgtctaccc
      421 ctgcgtttga ttttggaata ttcgcgcaga tgtttgagaa tatgaaggac gggttcggac
      481 cgataaccac cgttgagcgc aattacaggc tgtcgcactt tgcggtgcat ttttctcctg
      541 cgtattacct gatgctgccg ttctatatgc tttttccgca cccggtaaca ttacagatac
      601 tgcagggaat attcgttaca agcggaatta tccccgtgtt ccttatcgca agaaaatacg
      661 gcttttcgct tatacattcc tcgctttttt cagcgatata tgccttatat ccggcattca
      721 caggcggctg cagctacgat attcacgaaa actgtatgct tccggcgttc ctgctgtggc
      781 tgatatgggc tgttgaacgc gaaaagactg taccgatgct cgtctttgca ttgctgacgc
      841 ttcttgtaaa agaagatgcc gccgtatatg ttgcggtgat cgggctttat cttatactgt
      901 cagaccgcag tgaaaagaca agagcgctcg gtgctgtgat tttcggagcg gcctgcgttt
      961 atttctttgc agtatgcgct tatctcaata acagcgggct ggggatcatg gaatggcggt
     1021 acaaggacta tatgtacaga ggcggtgcgc ttatcacctc aatagtcgtt accgcgttta
     1081 caaatcccgg ctacattctg tcaaatctgt ttacgggcga aaagctgacc tttacggtgc
     1141 agacgcttgg tgtactcggc ggtataccgc ttgtaagccg aaagatagca cgttatatat
     1201 tactgatacc gcttgtactg gtaaatctga tgccgaccta tcagtatcag cactcgattt
     1261 atttccagta cgtttttggc tcgtgcgttc ttgttatctg gcttttcata atgaatatgt
     1321 cggagctgtc atataacagg gcgagatgct ttaccgtgtt ttcgcttatt gcctgtgcgg
     1381 tcatgtttat gtcaacaatg acaggctacc tgaacaattt ctatgataac gactatgagg
     1441 cacacggtgc cgtcatatct tatcttgaac agctcccgga caacgcaaac gagagtatta
     1501 cggcgaacac gttctttatc ccgccgctgt ataagcaaaa agagctgtat acgataaacg
     1561 atcgcgacga acctacaagc gaatccgcac cgcctgcgga tatagttctg cttgacagga
     1621 caaacgtaaa gtttgaaacc aattacaatt atttcacctc actcggaatg aaagaggtaa
     1681 cgatagagga cgaaagggtc gcatgccttg tatgcagg
//
LOCUS       DAWAOG010000484         1711 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9223_length_1711_cov_1.840580, whole genome shotgun sequence.
ACCESSION   DAWAOG010000484 DAWAOG010000000
VERSION     DAWAOG010000484.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9223_length_1711_cov_1.840580"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    903..912
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          420 a          374 c          359 g          548 t
ORIGIN      
        1 gctgacccag ctcgacaccg tatgccatat ttattttatc cgcatacttt ttaataaggg
       61 cgggagagat ctctgacgct cgttttacag gttcttcata gaggtattct tcatcgtaga
      121 atttatttat ttcaaggtga tctgtgagcg agtaatatct gataccaagc tctgtcgctc
      181 gtctgaccat ttcttccggt tcgttgcttc cgtcgggcga cagatttgag tgattgtgta
      241 cgtccatcag aatttctgat tcgttcattt ttgttacctt ccttttgcgg ttgctttata
      301 tctatacatt ttctatttta ccacaaacaa aaccgtttgt ctattgaata tgaaataaaa
      361 tccgttattt tgcaaaaaaa ccgaggaaat aaattccccg gttttcttta tttgttgttg
      421 tcaagatcat tgttgacacc gtatgaaagc gtcagcaaac tgtcgccgag gtgtacattt
      481 tccacgatta tgtttttgtg ctcaacgtta atatcatagt gggagaagtt ccagaacgat
      541 ctgacaccaa gctcggtaag tcggtctact atctctttag tgaccgattt cggaatgcaa
      601 agaactgcaa cctccggctt gttttcacgg cagaagtctt caaggtcgtc tatatctgtg
      661 atgttaagct ttccgagagg attgccgact atttttttgt tggagtcgaa tatgccaaca
      721 aggctgcatc cgctcttttc aaagttgata tgaactgcca aagcagtacc gagattaccc
      781 gcaccgataa gaatggtttt tctgtgcttg tcaactccga ggatacggcc aatctccttt
      841 cgaagctcgg aaacattgta ggcatagccc tgttgtccga aacctccgaa gcaattgaga
      901 tcnnnnnnnn nnctccgaca attcccttga agaaatttta gagatcagct gatcctccag
      961 ttcgccgaga aagcggtagt agcggggaag tctgcgtatt actgagttag atatgttgat
     1021 gcttttcgac aaggtactgc ctccttacag tttcttatat ttactgaatt acagagtttc
     1081 tttcagtctg ttattgattt cgagcaggaa gtcctctgtg ttgacctttc tcttgttttc
     1141 aagatttgaa agtacataaa ggtcgccggt cataacaccg tcttcaatcg tctttatagt
     1201 agccttttca agcttgtccg caaagtcgga aagctccttg ttgccgtcaa gctcgccgcg
     1261 ttttcttaat gcacctgtcc atgcaaacag agtagctacc gagtttgtag atgtttcttc
     1321 gccctttaag tgcttgtagt agtgacgtgt tactgtgccg tgtgcggctt catactcgta
     1381 aacgcctgtg ggtgaaacaa gaaccgatgt catcataccg aggcttccga aagctgtagc
     1441 taccatatct gacataacgt caccgtcata gttcttgcaa gcccagatat agccgccctc
     1501 agaacgtaca actcttgcta ctgcgtcatc gatcagtgta gagaagtatg tgatacctgc
     1561 ggcatcgaac ttttctttgt actcattatc gaatatttcc tggaaaatat ccttgaaggt
     1621 gtggtcgtac ttcttggaaa tagtatcctt agttgcaaac cagatatcct gctttgtatc
     1681 aagcgcgaag ttgaagcagg ctcttgcaaa g
//
LOCUS       DAWAOG010000485         1711 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9220_length_1711_cov_2.030797, whole genome shotgun sequence.
ACCESSION   DAWAOG010000485 DAWAOG010000000
VERSION     DAWAOG010000485.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9220_length_1711_cov_2.030797"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          513 a          393 c          417 g          388 t
ORIGIN      
        1 ccgtcgaaga taatctccga caaggagctg ttaaagcagg tgaccgcaac ggtaactgcc
       61 gttgaacccg atgtaacggt cagcgaaaag gaaaaccacg ttgagaaaaa ggtaagctat
      121 actaaagata tagtcaagat gataagcgca ggcattctgt tcatagtcgc ttttatcctt
      181 gaaaaaacta tcggtacaga acagcttgca ccgaaaatag cgatactttc aatgtatctt
      241 gcagcttacg ttatctgcgg acttgaggta gcgatcactt caataaaggc aatagcaaag
      301 aagaatttct tcaacgaaaa cacgcttatg cttatcgcaa gtatcggcgc tatagtgctc
      361 ggcgaatatg aagaagcggt tgccgttatg ctgttctaca ctgtcggcga gttcttccag
      421 tcaatagccg ttaacaagtc aagaagaagc atatcttccc ttatcaggac aaaacccgaa
      481 acagccgatg ttcttattga cggcgaatat ataacggtcg accccgaaaa cgttgaaaca
      541 ggcagtataa taagagtaaa gcccggcgaa aagataccgc ttgacggcgt tatcgaaagc
      601 ggtaacacct caatagatac ctcagccctc acaggcgaga gcttaccgcg agatattacg
      661 gtcggtgatg aagtccccgc aggtgctatc aatatttcag gcgttatcac tcttaaaaca
      721 acgcgccggt tcgccgacag cacggtatac aaaatgcttc agatggtcga gtccgctgtc
      781 gagaaaaaga caaagacaga aaacttcatc tcggtatttg caaaatacta cacccctata
      841 gtcgtacttg cggctgtgat catctctctt atccctcccc tgttcacagg ctttgacttc
      901 ggaacgtggg tacaaagagg tcttatcttc ctcgtaattt cgtgtccgtg tgcactggtt
      961 atctctgttc cgctcggata ctttgcaggt atcggcaagg cttcgtcaaa gggcatactt
     1021 gtaaaaggca gcaactacct tgaagcaatt tcaaaagcaa aggtcgtact tttcgataag
     1081 acaggcacgc ttacaaaggg tcagtttgaa gtgacgaaaa ctgaacctgt cggcatgagc
     1141 aaggaagagc tgttacgata tgcggcatac gcagagagta actcaaatca cccgatagcc
     1201 gtttccgtaa gaaaagcata cggtgcggat atcgaccagt cacagatcac cgaatgcagt
     1261 gagatagccg gcaagggcat aaaggctgag atttcgggcg cgaccgttct ctgcggtaac
     1321 agcagactta tggctgacta cagcatagac tgccccgaag caaacggcac agtgctttat
     1381 gtggctgtcg acaataaata tgcaggtctt atcgaaatcg cagatatgcc gaaggaacat
     1441 tcggcagaag ccgtaaagat gctgaaaaat cacggcgtaa aggtcgtaat gcttacaggt
     1501 gataataaat ctgcggcggc tgtggcggca gaaaagctcg gtataacaga ctactacgca
     1561 gagcttctcc ccgaaaacaa gagcgagatc acgcttaaga tgaaatctga gctttccgac
     1621 aaggaaaagg ttatgttcgt cggtgacggt ataaacgacg ctcccgttat cgcatcagcc
     1681 gacataggcg ttgcgatggg cggctcgggc g
//
LOCUS       DAWAOG010000486         1700 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9297_length_1700_cov_1.781155, whole genome shotgun sequence.
ACCESSION   DAWAOG010000486 DAWAOG010000000
VERSION     DAWAOG010000486.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9297_length_1700_cov_1.781155"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          511 a          367 c          412 g          410 t
ORIGIN      
        1 ggcgtaatag ccgtagtgct gaatattctc ggacttattt cgctgataac taccgaaact
       61 accgatgtaa gctcgataat gggcatattg atattctgga tggtgttcac ggttatatgc
      121 tacatagtgc ctgtattctt tgcgatgtta aaaaaatatt tcatttgcgg cggtcttaca
      181 tttgcgggca tgatatgcgt acttgttctt cacgaactgc tcccgggcga tcccggatac
      241 ctctatatgc cgctccttat aataacgata ctcggcatac ttcttgcgat attcggcaac
      301 tgggacaaga tacacggaaa tattgaaaaa cgcgaaaagg aaaagaacaa ggccgcgccc
      361 tctgttctgg gcggcactac cgctccggct gacgaagtga tttcaaagaa agtcgcaaaa
      421 aaacaggcgc gagcaaagaa ataagcgttt ttacgaaagg gtgataatat ggcacttctt
      481 acagaaaacg aacagttcca tcttaaaatc aaaaaaggag atgtcggaag atatgctatt
      541 cttcccggcg acccgggcag agtccctctt atagcaaaat atctcgacaa cgcggaattt
      601 atcgcttcaa accgtgaata tacgacctat accggttatc ttctcggcga aaaggtaagc
      661 gtggtttcaa cgggtatcgg aggtgcgtct gccgcaattg ccgttgaaga gctaatccgc
      721 tctggcacgg acacctttat aagaattggc acaagcggcg gtatggatat cagtgtttcc
      781 ggcggcgatt taatcgtggc tcaggcatca atacgcagtg aaggaacatc acacgagtat
      841 attcccgaaa actaccctgc ggtagcggat tttgaggtaa ctactgcgct taaagccgca
      901 ggcgacgcat tatcaaaaga tgttgacggc aagcgttgcc atgtcggagt agtacacagt
      961 aaggacagct tttacggcga gatagaaccc gagcagatgc cggtcggaga taagctctcg
     1021 ggcaactggg cggcttatgt aaaatgcggc tgcctgactt ccgaaatgga gtccgccgct
     1081 atattctcgg tagcccttgc aagaagaaaa cgtgcaggcg ctgtatttac agccctatgg
     1141 aatgttgaaa gaagcaacgc gggactaccc gatactgtct gtatggacaa tgacagagcg
     1201 ataagaacgg cagtgaacgc agtaaaaata cttatagaac aggacagaaa aaatggcatt
     1261 tgaacatata ttattcgacc ttgacggaac gcttacagat tcctatgagg gtatagcaaa
     1321 atgcgtacaa tatgcgctga gctattacgg aatagaggaa aacaacgagg ataaccttaa
     1381 aagatttatc ggtcctccgc tgtgggaatc attccatatg ttctatgact tccccgaaga
     1441 aaaagcaaaa gaggcggtgc tgaaataccg cgagcgttac aatactgtcg gagtgtacga
     1501 aaacaaggtc atagcaggtg ttcccgaaac actgaaaacg ctttatgaca acggcaggaa
     1561 aatatacctt gccacctcaa aaccgctaaa gcttgcgaat gtcgtgctcg aatattttga
     1621 tctggcaaaa tatttcacat tcatctgcgg tgcatcgctt gatttttcgt ttgaggacaa
     1681 ggcaagcata ataaactatg
//
LOCUS       DAWAOG010000487         1698 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9309_length_1698_cov_2.412051, whole genome shotgun sequence.
ACCESSION   DAWAOG010000487 DAWAOG010000000
VERSION     DAWAOG010000487.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9309_length_1698_cov_2.412051"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          449 a          366 c          357 g          526 t
ORIGIN      
        1 ccaattttta ataattaaga ggtttttcga cagtctgtag cgggttcgta ttaagaaccc
       61 gctgatgcat tttttctttt ttcgcaccgt gactatcgac aaagagcctt tcttttcata
      121 tttgtgtcac agcaattgtt tttttatatg tgtataacag atcgtcggtt attttctgta
      181 atatgagttg aatacaggtc ttaacgcctt ataaagcggc atacagagca ctgaagccac
      241 aagacccttt ataagagtga acgggcagtt gaatacgata aggcattcga gaagaccgtt
      301 tacgttcgga tttatttcct tgtacatatt gatgatagct tcgataggca taatgttttc
      361 gtatatcggg tataccagga aatagtttga cacgatactg agcaatgcca tcacaacact
      421 tcctacagcg caaccgatta ttgcaccctt ataggtgttt atctttctgc cgattaagcc
      481 tgcggggata acaaggcaac agcttaagat gaagttggaa agctcaccta caccgcctgt
      541 catacttgaa aacagattga ttatgttctt taccaggcat acgaatacgc ctgccacagg
      601 acccatagta agtgaagcca ttactgcggg aaagtccgag aagtcaaacg agataaagct
      661 cggcattagc ggtactttga tactgaacag cataagtacc gatgatatag ccgcaagaag
      721 tgccgtaaaa acgagctttc ttacatcaaa gacaatatgc gccctgtttc ctgttgtttg
      781 tgtctgcata aaaatcatcc tttctatatc tgctccaaag gtgactttca gcttttcgcc
      841 aaaaaataaa agccccgtat tatcggggct ttaaccttat ctcaaaagtg ttgaaccgct
      901 tatatataaa agatttgcgg tcaagcattt ctgattcttt catccggact ataccgtcgg
      961 tttcggaatt tcaccgaatc ggcgcatttt gttatgcgct cgtggacttt accaccggtg
     1021 gagaatttca ctccgccctg aaacagatta cgattatatt atatgcctgt gcggctgttt
     1081 tgtcaagagg gaaaaggaaa taaagttgaa attatacttg atatgtatca gctgttcatc
     1141 ttctctgcct ttttctcaag agccttgata tcaagtccgt acttctcaaa tatcgttgcg
     1201 gcatagctgt tagcgccggg cagacctttc tttattttgt atctgcgttc gcctgttgct
     1261 tcatcaacag ttacgattat gctttcggtc cttgttttta gcttagggtc ggaattaagc
     1321 tcaagcgctg ttttagcaag cggtgtaagg tgagttgcaa atacacctct tgcgccgata
     1381 atgcaaaaga tacgcataag ctgtgcggct atatctgtac attcctgcgg tgtggtactc
     1441 tgtatcgact cattcatcaa aagcaggctg tggttagtta tgcagtcgct tatagtctta
     1501 aattctttta cctctgtagt gaaacggctc gcatctatac cgacctgttc ttcttttgga
     1561 aaatgtgtgt atatgcagtc aacaagcgat atctcacagc tgcttgcggg aacgtaaagc
     1621 cccgcctgag ccatgacctg acatattccg accgcacgga cgaaggtcgt tttgccgccg
     1681 ttgttcgcac ctgtaagt
//
LOCUS       DAWAOG010000488         1687 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9397_length_1687_cov_1.999387, whole genome shotgun sequence.
ACCESSION   DAWAOG010000488 DAWAOG010000000
VERSION     DAWAOG010000488.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9397_length_1687_cov_1.999387"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          483 a          365 c          421 g          418 t
ORIGIN      
        1 gacttctgcg tgaaagagag agcaaaaata acgatataaa gacaggtaac gttgagatac
       61 tggttaccga cctgcgcata ctttcaatgg cgcagacaac accgtttgag atagtaaacg
      121 atacaaagac aaatgaggag ctccgcctta agtaccgata tcttgatctt cgcagaaaga
      181 agttacagga taaccttata atacgtcaca agatagcaaa gacagcacgt gagtattttt
      241 atgataacgg ctttcttgaa atagaaacac ctatgatgat gaagtctact cccgagggtg
      301 caagagatta ccttgtaccg tcaagagttc acgagggtaa gttctatgcg cttccccagt
      361 cgccccagat atataagcag ttactgatga tctcgggctt tgaccgttat atccagcttg
      421 cgagatgctt cagagatgag gatctgcgtg cggacagaca gcccgagttc acacagatcg
      481 accttgaaat gtcatttatg gacgttgatg agatacttga acttacagag ggctttatca
      541 ggaagctgat gaaggatgtt cttgatgttg atatcacaac tccgctcccc agactgacct
      601 ataatgacgc tatgtcacgt tatggctcgg ataagcccga tacacgcttc ggaatggaga
      661 tacaggatct gtccgatatt gttaaggata ccgagttttc cgtattctcc tcggcaattg
      721 ccgcaggcgg aagtgtaaga gctatcgttg caaagaacgc cgcaggcatt ctcacaagaa
      781 aagagatcga caagctgacc gagttctcaa agggtatcgg cgctaagggt cttgcttata
      841 tccgttggaa tgatgatgcg ccgaactgct cgtttgcaaa attcctcaaa gagggcgagc
      901 ttgacgctat cctttcgcac ctcggctgtg aaaagggaga tgtagtgctg attgttgccg
      961 ataagaataa ggtgacgctc cctgtactcg gtgctctgcg tcttaaggtc ggcaagcaga
     1021 taggcattgt acccgacagc ggctacaatt tcttatggat aaccgagttc ccgttctttg
     1081 agtgggacga tgaatcggaa agctggcttg ctatgcacca cccattcacg atgcctatgg
     1141 aagagtgcct tgaatacctt gacaccgaca agggcaaggt aagagcgaag gcttatgacc
     1201 ttgtactcaa cggcatcgag ctttgcagtg gttctatccg tataaccgac cctgagcttc
     1261 aggagagaat gttcagactg ctcggtctta cagataagga aattgaagag aaattcggct
     1321 tccttgttga cgcatacaag tatgccgcac ctccgcacgg tggtcttggt ataggtcttg
     1381 acaggctttc gatgctcctt tgcggtgcag acagcctgag agatgtagtt gcattcccga
     1441 aggtaaagga cgcaagcgag cttatgtcgc agtgcccgtc agtagttgac gataagcagc
     1501 ttgaggagct tcatataaga acggttgttt ccgataagca ataaatcgtc caagccatta
     1561 ctgagaaagc atacgaggaa atgtaaatga ataaagcatc atctgccgcc gctaaaaaaa
     1621 acaacggcat acaaagaagc ttaagacact gtgtagagct tgtattctgt ataactgcag
     1681 taatgat
//
LOCUS       DAWAOG010000489         1683 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9422_length_1683_cov_2.258600, whole genome shotgun sequence.
ACCESSION   DAWAOG010000489 DAWAOG010000000
VERSION     DAWAOG010000489.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9422_length_1683_cov_2.258600"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    887..896
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          364 a          443 c          346 g          520 t
ORIGIN      
        1 cgatcctctc cgcacggaga tccgtgcctt tctcttcctt tattttaaag gaaatcccgc
       61 gctgtgaaag cgcattgcgg ttagctccgc cctgtcctat tatcttgctt atattacgct
      121 tgtcagtgta gaaaatatat tttcccccat cctcacacac ggcgttcatt ttatcaagat
      181 acaagatgct ctctgctatc tctcgcagtg ccgggtgata tactccgccg atcatttcct
      241 gctcaacgtc acggcttgcg tgcagtccca tacggataac ggcaactccc gctttattga
      301 acatatcgag cagctttgcg catagccttg tcgtctgctc aaatgtaaag ctgtcataca
      361 caccgctttc gtgcagtctg ccaagctcgg tgtttttcag tataacagta gggtaaattc
      421 tcaccgtgtc gcagtgaagc gcgataaatt cgtttgccgt atcgatacaa tactgctctg
      481 tatccttata cagtccggtc atcatctgaa gcccgagctc gaagccgtac tgctttataa
      541 gccgcgatgc acgccttacg tcttcagctg tgtgtcctct gcggttagct gtcagcacct
      601 cgtcattcat actctgcgca ccgagttcta tcgctgtcat tccgtatttt ttcaggatat
      661 caagtatctc ctcatcaatg cagtcgggtc ttgtagaaca gcgtatgccg tcatactgct
      721 cgggatatgc gtctgtataa cgcttggcta ctgtcagcag tcttatcata taatcgcggt
      781 ctattgcggt aaagctgccg ccgaaaaacg ctatctgcgc ctttgttccg ctgtttttca
      841 gattttcaag ctgactctca agtatgcttt caagctcctt ttcggtnnnn nnnnnntatc
      901 gtcttctgat tgcagaagct gcatatatgc ttacagccgt aatgcggtat aaataccgaa
      961 atgttagttt gtttcatatc ccataaggct tattgcctct tttgcggcgc tctgctccgc
     1021 ttccttcttg ctcttgccct tgcctgtacc gattatctga ccgttcagaa gcacgtttgc
     1081 ggtgaactgc ttgttatgcg cctgtccgca ctcattgctt atctcataag tcacacgctc
     1141 ctcggggttc tgctggatta tctcctgcaa caccgtcttg taatctccga gcaggaattt
     1201 acccgtactc agcttatcga gcggagggat aaacggcagt atgaaattcc ttgccgcttc
     1261 aaatccgccg tcaagatata ttgccgcaat aagcgcctca aatgcgtccg cgagtataga
     1321 gtggcgatct ctgccgcccg tatgctcttc tccttttccc aggaaaatat acttgcctaa
     1381 atctattttc cttgcaaagg gataaagcgc attttcacac acaacgcttg ctcttatctt
     1441 agtaagctgt ccctcgggca ggtttgtaag attttcaaag agatgctcgg acacaacaat
     1501 gctcagcacg ctgtcgccta aaaattcaag gcgctcgtta ctgcgcagat gtttaccgtt
     1561 tgtgtatgac gaatgagtaa gagcttcttt taaaagctcg ggatttttga atgtatagcc
     1621 tattatctgt tcaagctctg tcatttcttt tcctttcaat aattctcacg ggagggaagc
     1681 ccc
//
LOCUS       DAWAOG010000490         1683 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9423_length_1683_cov_1.787469, whole genome shotgun sequence.
ACCESSION   DAWAOG010000490 DAWAOG010000000
VERSION     DAWAOG010000490.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9423_length_1683_cov_1.787469"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    226..235
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1088..1211
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          379 a          374 c          321 g          475 t
ORIGIN      
        1 ggattgttct gatccatgaa ctgtgaaagc ggagaagaac cgaagaactc cttgatagcc
       61 gctacaacag gacggatatt tataagagca ttaggtgtca ccttgtcgcc ttcctgcgac
      121 tgaagaccca tacgctcgcg gataactctt tccattctcg caaagccgat tctgaactgg
      181 ttctgtaaca gctcgccgac actgcggata cgtctgtttc cgaggnnnnn nnnnngttga
      241 taaagtagct gactgtagca aatatatcat caagagttat gtgcttgggg ataagatcct
      301 caactctgtt tgaaagctct tccttaagtg cctcgacatc atctccgcat ttatcgagta
      361 tttcacgcag taccttgaaa caaaccttct cattgatacc gagagcctca gcgtcgatag
      421 attcggggat atatcctgcg atatctacca taccgttgcc gagaaccttt gcgatcttct
      481 cttccgagaa gataacgcat tccataacgc ctgcctgctc gatcatctga gccttttcag
      541 cgttgataaa ttcgttttca tcaacaagta tctcacctgt tgtaggatct ataataggct
      601 gagccgcctt atgtcccgca attctagatg caacaccgag cttcttattg tacttgtaac
      661 gaccgaatct tgcaaggtca tatctcttgg catcaaagaa cagattatta agatgcgtct
      721 tagccgagtc aaccgtggga ggttcgcccg gtcttaactt tctgtatact tcaagaagcg
      781 cttcttcggt attctttgtt gcgtcctttt cctgaatagt ctgcactatt ctgggatctt
      841 cgccgaacag atcgataaga tcgtcatcac tgcttatacc aagcgcacgg ataaatgtcg
      901 tagcgggtat ctttctgttc ttatctatac ggacataata tatgtcgttt gagtccatct
      961 cgtattcgag ccaagcgcct ctgttgggga taacagttga cttgaatagc tttttaccgg
     1021 tcttatcgta gtcaaagcca taataaacgc cgggagaacg tacgagctgt gaaactatgg
     1081 cacgttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn natgtgccgc tgtcagtcat cagagggaag tcgcccataa atatctcatt
     1261 ttcaacaact tcacccgttt cggtgtttct gagctgtgcc tttactctta agggtgcggc
     1321 gtaagttgta tcacgctcct tgcactcttc tatagtatac ttcggggtct cgtcgatacg
     1381 gtaatcaacg aagctcagta ccagcttgcc gttagcgtcg gtgatagaag aaatatcctt
     1441 gaatacttcc tttataccct cgtctaaaaa ccactgatac gagtccttct gtatttcaat
     1501 gaggttcggc attttctgaa cttcattgat cttggaaaaa ctcatacggg tatttctacc
     1561 cagttttacc tgcttgacat tcaccatggg ctttaccact ccttaattta ttcgtaggaa
     1621 ttactgcatc ggattttccg catagcactg agcttttcgg cgcttttcga catatgtcgc
     1681 ttt
//
LOCUS       DAWAOG010000491         1679 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9442_length_1679_cov_2.033251, whole genome shotgun sequence.
ACCESSION   DAWAOG010000491 DAWAOG010000000
VERSION     DAWAOG010000491.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9442_length_1679_cov_2.033251"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    460..469
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1129..1138
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          406 a          398 c          371 g          484 t
ORIGIN      
        1 gtgtagcgta gctggttaac gcgtcggatt gtggtcccga agatcgtggg ttcgaatccc
       61 atctcccacc ccatatttat tatgtacaaa tgccgcctgc gctgggctta agtgccggcg
      121 gcatttgttt ataatgggat atagccaagc ggtaaggcaa cggactttga ctccgtcact
      181 cgtgggttca aatcccgcta tcccagccag atactgttta taggggctgt gacacagccc
      241 gaaaaaggaa aagtgagtcg aagcgcaaag aatagtgcgc acggctcact ttttggtatg
      301 tggagaaagt ctgacctaag tacactcctg ttatgccgtt ttatcaatca acattgacag
      361 caggctaccg atagttcagt ttgtcggata gcgcttctcg ggtttgccta tgcgataacg
      421 ctgctaatat atttgaggtt cgcaaagcat tctgaaatan nnnnnnnnng ctttacttgt
      481 gttgtatcgt tagtcgtaat cgtgttaatt aaacaccgta cttagctgtg cttaccttaa
      541 cgccggggcc cattgttgaa gaaactgtac agctcttcag atatgcaccc tttgcagcgg
      601 cgggcttagc cttaactata gcgtccatca gtgtgttgaa gttctgttcg agcttctcct
      661 taccgaatga tgccttgcct atagggcagt ggattatgtt agacttgtca agacggtatt
      721 cgatcttacc tgccttagct tctgaaattg ccttagcaac atcaggagta actgtacctg
      781 ccttggggtt aggcataaga ccacgcggac cgagaacctt acctaaacgt cctacaacac
      841 ccatcatatc gggagttgcg attacaacgt cgaagtccat ccaaccgtcg ttgatcttct
      901 taactgtatc atcgtcagca acgaaatcag aacctgcgtc gatagcagcc tggatcttat
      961 cgcccttagc gaataccagt gtgcgtacag tcttacctgt accgttgggc agtacaaccg
     1021 cacctcttac ctgctgatca gcgtgacgtg agtcaacacc gagtctaacg tgaagctcaa
     1081 ctgtttcatc gaacttagcc tttgcagtct ggcaaactaa gtcgagagnn nnnnnnnngt
     1141 atgctttagt gctgtcgatc agctttacgc tgtcaacgta tttcttacct tgtttcattc
     1201 attatcctcc ttgtggtata acggaaatta tcctcccacc gtcttaatta gtcaactact
     1261 tcaataccca ttgaacgagc tgtgccggcg atcatagaca tagcggcttc tatagaaccc
     1321 gcatttaagt cacgcatctt tgtttcagcg atctgctgaa cctgtgcctt agtgatctta
     1381 gcaaccttgt tcttgttggg tgtgcctgat gctgtttcaa ttccgcaagc cttcttgata
     1441 agaacggcag caggcggagt ttttgtaata aatgaaaaac ttctgtcagc ataaactgtg
     1501 ataacaacag gtataataag acctgcgtca ttctttgtgc gctcattgaa ttcctttgta
     1561 aacgccataa tgtttacacc gtgctgacct aatgcaggac caaccggggg agccggcgtt
     1621 gctttaccgg caggaatttg taattttata aatcctgtaa ccttctgagc cattttact
//
LOCUS       DAWAOG010000492         1676 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9470_length_1676_cov_1.853794, whole genome shotgun sequence.
ACCESSION   DAWAOG010000492 DAWAOG010000000
VERSION     DAWAOG010000492.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9470_length_1676_cov_1.853794"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          444 a          374 c          382 g          476 t
ORIGIN      
        1 tcaacacctg tgtttgtgct gggctttgta gagtcgcctg ttgtaggagc atctgtagcg
       61 ggagcatctg tagcaggtgc atctgtagcg ggagcatctg tagcaggtgc agagttgcca
      121 ccgtcaacta cattgccaag accgtcgaat gcaataagta cattgccttc agcgtcgaga
      181 acttcacact ttacaacttc aattgtagcg atagacgagc cccattcctg gagtgcgacc
      241 tgcatgcaac cgattgtctt agctgtgccg ttagcgatgg ggttgtcaaa gccgttagct
      301 gtcagctggt atgtataatc gtcgatcttc tcgtagctca gatccttatc gtttgaagca
      361 tcgtcatcct tgttggggtc tgtagcgagc tcaagagcct catctgtaac tccccagtac
      421 tccttgctgg gccagttata tgtatcccaa gcgtctgtac cctgctggat gtcgccgccg
      481 ttgattgaaa gaataacgcc gccgcctgta gcaccgtccc agaactcacg gttgccgtca
      541 gcaccgtcag catccttttc gggaattgta acagtaaatc ttgcgcctgt tacctttgag
      601 tagtcgatat catactttgt agcgggcttg ttctcagctt catttccttc gttgaaaacc
      661 tgaacgagcc atgaatcgtt acctgatgaa aggcctgcag acttgccgtc tgcttctgtt
      721 accttcttaa ggcttgatgc gcttgctgct acagctaatg tgctgagtgc gagcgaagaa
      781 gctacgatac ctgtcatgat ctttcttaac ttcattggaa aaatcctcct aataaaactt
      841 aatttcgttt tgttacatac ttgtgtaact ttacacacct attttaacat ttcgtttttt
      901 taaagtcaat gtgcattatg cctaaaatgt atgtgaaatt ttattgaaac taacggcatt
      961 acacagcatc aagggcataa tttaagtaaa tttaagatta acggtgaatt atgttgtgtt
     1021 tttgtgataa aaagagtggg tggaaaggtg ctataatata ccgcttatgc aacggaattg
     1081 ttcggggatt gcttgaggat tgttcgacaa aaacgaaaac tgcttaaaat tttacttaaa
     1141 aggattgaga aaagccgaaa aatatggtat atttaatgta tatgggaact tgttgtatcc
     1201 cgaataagtt aaaggacgga aaaactatgt ctatcgtatt tgacgaaaac aagcgtgtat
     1261 tcaagctcga cacggaaaaa tcgagctacg cattccatat caccgacagc agaaacctgc
     1321 tccacctgta ctacggcggt tatattccgg aaactgatat aacacatatg ctccgtatac
     1381 cggacgatga accgtttgtc cccgccgtac atgacgctat gggacctcac agctttgact
     1441 gcgcgcctat cgaatttccg acatcgggcg ttgcggattt cagagaaccg gcaatgcagg
     1501 taatggatat aaacggtatg tccgcctgcg agtgctacta caaggattac agaatttcaa
     1561 acggaaagcc gaaattaaag gggcttcccg ctacatatgc cgcagacgat gaggcgcaga
     1621 cgcttgaggt gttctgctac gacccacaca gcggtcttga cattacactt atgtac
//
LOCUS       DAWAOG010000493         1674 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9488_length_1674_cov_1.949351, whole genome shotgun sequence.
ACCESSION   DAWAOG010000493 DAWAOG010000000
VERSION     DAWAOG010000493.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9488_length_1674_cov_1.949351"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    655..707
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          368 a          387 c          363 g          503 t
ORIGIN      
        1 ccgctgccgt tgaaggttat atccgacttt gaaaatacag caccgtcaac gtttgtttca
       61 ttatcagatg aggtaaattc acctgtgccg gtgagtgtgt tttcagtacc gctcacggtc
      121 gttacaaata ccttgtcggc atttatagca tacagagccg cactgccgtc tttggttata
      181 cttgcgttgt caagaacaag ctgtaccttg tcgctgtcgc ccgcattcac gattatctga
      241 cctgtgaatg tgccgctgag cttatatgtt cccgctttcg ttatagtaat cgagccgtct
      301 gctaccgtta ccgagctgtc cgggcagctt gccgcagaac cgctgagcgt tatgtcaacg
      361 cattcggagt attctcccga caggtcgcgg tcggagaaca tatcatcggc tgtaagctga
      421 ttttcggtgt tatcggacga tgtgctgccg tcagttttgc tgttggtgct ttccgcactg
      481 tctgagctgt ctttgctttc gttactgctt gatgcgctgt ctgaagcact gctgtcagtt
      541 acatcggata cactgcttgc ctgctgagag gacgaagcgg tcgatgctgt gccgttgtcg
      601 caggaagtga ataatgaagc tatgagagct gtagttacca ataccgcaag aattnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct ttccggggtt
      721 acagcatttc tctgctgtca gtgccctgtg aagatatatt tatttcaagg ttgccgtttc
      781 tgcatctgag ctcatctatc atctgctttt cgcagcctgc tttacctagt gtgatattgt
      841 aggtgagctt gttaaggctg ccgagatttg tggtctttac ctgtgtcatt ttgtactgcg
      901 ataagtattt accgaatata tcgtcaaatg cactttcata ttcaagattc tcgggaattg
      961 ttatatgaag catcttgtta agctcgtttt tctttgactc gccgaaggga gaagctgtaa
     1021 gtatgatgtt cgccgcaccc atgataactg cgaatataag ggcgtataac ggataaccca
     1081 taccgcaggc aagtcctacc gccattgcaa ggaagataga gcctatttcc tttgccgtac
     1141 cgggcacgga gcgaaatctt acaagtgaga atgttcccgc taccgcaacg cccgcaccta
     1201 tcgagccgct taccatcata attactatgc tgacaactgc ggggagtgtt gcaagagtta
     1261 tggcaaagct ctttgtgtat gtgtttttat acatatacat aaatgccatt gctataccaa
     1321 gtaccagtgc cgagccgaca ccgataagaa aaccgccgag tgttatgcca gtcgcattgc
     1381 tgaaaattcc ttctattacc gtatcaaaca tttaccgtta cctcacttct gatttttttg
     1441 tttttaagcc catgctcctt ttgagcattg tcatatatgc cattccgtac tttgagaacg
     1501 aagtttgctt tatttcgcct tctgctagta attcaaccaa ccacatcggc attgagcctg
     1561 ccgcctttat ttccatcaga gaacagccat tttcaagaat ttgctcaccg tagggagcta
     1621 ttttcagatc tacgtcttct gttctccaac gtatattgcg gtcgaacgta actc
//
LOCUS       DAWAOG010000494         1660 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9600_length_1660_cov_2.529595, whole genome shotgun sequence.
ACCESSION   DAWAOG010000494 DAWAOG010000000
VERSION     DAWAOG010000494.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9600_length_1660_cov_2.529595"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          518 a          311 c          348 g          483 t
ORIGIN      
        1 gtatactata gtcacagtca gaggaaagga ctccgacgac cgaagtcagc cgataggttt
       61 cggctcgaag aataccgcgc caataagcgc ggtttacata acaggagccg gcaagacggc
      121 aacagccgcc ctatacacgc tccgcctaag tacaacgaaa gttttcatat atacctctct
      181 attattcaag caaaaccgct ctgtaacgca gggcggtttt tgctgtactc tcacgctccg
      241 cctttccttt tgctgcaggt tgaaatttct gcaattcggt gctataataa agatattgtt
      301 tttggaaaag attccggaaa gggtaaagac atgggatata tacttaaaac agcacagtac
      361 gtcaacgatt ttcagtgtat aggcggaaag tgtacgatgg attgttgccg aggttgggct
      421 atattgtgga cagatgaaga agtagaaaat ctgaagaact ccaaacactc agatgaatta
      481 gatgagctga taaaggcttc atttgaaaag gttgataacg gcataaataa aatagctctt
      541 ctctctgacg gtgactgtcc ttttcatgat aaagcagata ggctttgtaa aattcagaaa
      601 gaacttgggg cagaatacct ttcggctgtg tgtaaaagct atccgataag tggagttatt
      661 aacgataata taataactaa agtaagatgt atcagttgtc ctgcggtatt tgatttagtc
      721 ataaaagaag aaaaatcctg tgacgtttat atttacggta aagactatga accggaaacc
      781 attctgatat ttaaacccga tacaattgac gacgtaaaaa acaatccggg acttaaatac
      841 agaaatcagc tttttgattt tttctacggc attcttagtg acaaaaaacg tgatatacat
      901 acatcgatga ttattggcac tcttgccgca cagacgcttt caaagcttga aaattcatcg
      961 ccggacagga taccggaggg cattaccgct ctgtcaaaac agataaacag cccctcactt
     1021 gtaaaatctc tttcggatat tgaaccgaat tataaggtta agcttggcgc tgtgcagtgt
     1081 atgcttgacg gttctgccgt aggtgacaag ctctccgaaa tattgtcgtc aatcagaaaa
     1141 aagaacatcg aaggcaaatt ttccgttgaa atacaaacat atatagctaa tctcgaaaag
     1201 ttctataaaa tatgttcaac taaaccgttt atgataagaa acataataag atgtctttat
     1261 atgtgtgagc ttatgccgtt ttcggataat aattataata tttttgataa ctacgcatat
     1321 ttttgtgctt ctgcagcact tatagacttt ttcggcgctg ccgcagcagc cggcagtgat
     1381 aatcccaatg aaatatttga gaggtttaaa accgctgtct gtcttgcttc tcattcgctt
     1441 tgtcataatc ctgacagagt gaagatgata atagattact taaaggatat agactgctat
     1501 tcggcaggtc accttgcact tatcgtaaaa taagattttg tataaccgct ctgtgacgca
     1561 gggcggtttc agtctgtcga taaacctata tttttttgaa aaaggggtgt cgagtattgc
     1621 ttgctctgct ggttttgcat cgtgcaaaac ttttggcaag
//
LOCUS       DAWAOG010000495         1656 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9641_length_1656_cov_2.028107, whole genome shotgun sequence.
ACCESSION   DAWAOG010000495 DAWAOG010000000
VERSION     DAWAOG010000495.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9641_length_1656_cov_2.028107"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          499 a          344 c          423 g          390 t
ORIGIN      
        1 gtcttctcag ctgtatattg cgcgtatact atacgggcgg tcagaattta ttgttcacgt
       61 cagacacaga aaagctgtac tatgatgcgg ataagggtac ttatgtaagc agaaagtaca
      121 tcggtgacgt aaatgtatac tattatcgca atggcgtaga cttcccgctt gaggataagg
      181 acaagacagc aacactcaag tttgcggtat cggctgacgg caagacggaa acagtcgaca
      241 ctgtgacagt acagaagggc ggatatgtaa gcggcttctc gcttgcagtt gcaaactact
      301 gtaattcaaa cggctatatc tatgacacgt cgctgtttga caccgagata aagccgtact
      361 actggctcag tgaggatacg gtcataacgc ttaataccca caccgcgcag aagctgtact
      421 cggtggaata ctttgactca ggaattgaca aatgggtaag cgagctgtgg gcaacagact
      481 atctcgatga cataaagatg tttgacagat ataaagcgcc tgtgaacagt atcacccact
      541 ttgcaaactt cggtatcgac agagcagacg gtatgagata tcagatgtat atgaaggatt
      601 ctctgaccta ccgttactat gacagcagtt attccggcag cgtgtacggg ctggatacta
      661 caaaaccgct tgccgtaaat tacggcagcg aagaagagct gctctctttc agagcggaat
      721 acgaaaagca gtatccgact gcctacagca ttatgctgaa agccaactat ctgtcgagaa
      781 aatgcataat cgatatttac aatgaaagcg gctatgcagg tgcaagatat gttgcttacg
      841 gcgatgagtt cactgtaccg gatacattca tcgacgacct gaacagcagt aaggataaag
      901 agcttgtcgg ctgggatatt gacggtgacg gcaatgctga tatcgcccct gacgaaaaaa
      961 taaccgtaaa gggtgatatg aggctgaagc ctgttatgaa gaaaaagctg tacacggtaa
     1021 cggtaagact tgcggattat accgaaagaa agtttgacgt tatttacggc aagcctttac
     1081 cgcaggagct tgaccgactt ataaacagca ttcccgacag tccgaagccg gacagcgagg
     1141 attcgttcta cgagccgtca tatgtgctta ttaagagcga tgttgcggta ggcggttata
     1201 cggcaggcga aactgtaagg tacaacggcg atataagcat tatgcccgag ggcaatatgt
     1261 actttgagat agttcctgcg aagctgtatc actatataac gcttatcgca aacgagggcg
     1321 gcggcttcag ctatacggat gaaaacggcg ctgaaataga atcagacagg ataaggactg
     1381 ttgtacagga cggttaccgt ttcgcaacgg cattttacgg atattccgca ttcgcacctg
     1441 aggacacata cactaccgaa tatctgctgt cgtattttgt ggacgcagac ggaaacagga
     1501 tcggtactaa cgatattgtg aagaagcccg gcacttatta tatgaagtgg aacaccaacg
     1561 acagacgatg cctgataaac tatgacgttt atatttacaa cgagcacgga gcggaggttt
     1621 cacatgagat aaaggctgag tttgagggct acagac
//
LOCUS       DAWAOG010000496         1655 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9650_length_1655_cov_2.033125, whole genome shotgun sequence.
ACCESSION   DAWAOG010000496 DAWAOG010000000
VERSION     DAWAOG010000496.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9650_length_1655_cov_2.033125"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          481 a          360 c          429 g          385 t
ORIGIN      
        1 aagagagctt caggaagaaa caggctgtac ctgcaaggag ttcagataca tgggatatat
       61 gatccctact cctgcttact gtcaggagat aacacatatg taccttgcta caggtcttga
      121 gggcggaaag cagaaccttg acgatgatga atttctcgag gtggttaaga taccgcttga
      181 caaggctgtt gaaatggtta tgaacggcga aattgaggac ggcaagacac agcttgcttt
      241 actcaaagcc aagatgatat taggctgatt ttcggcataa aatattataa tgtcagactg
      301 aaaggatcgg gagtacaatg gaagatttcc tcacaggaaa gaaacacgtt cattttatcg
      361 gaataggcgg aagcggtatg tatccgctgg cgcagatact tcacagcaaa ggtttctatc
      421 ttacaggttc ggacaataac gaaacggaaa cgcttaaagc ggtgcggaag atgggtatac
      481 ccgtatacat gggtcagaga gccgagaata ttgagggcgc agatcttatt gtgtacacag
      541 cggctataat gagcgacaac ccggagctta tcgccgcaaa agcaagcggt gtgaagacta
      601 tagagcgctg tgagcttctc ggacttgtaa caagctggta caacaaggct gtatgcgtat
      661 cgggcacaca cggcaagact acaacgtcct ctatgctgac gcatatgtat cttgcgcagg
      721 acattgacct gtctaccgtt atcggcggaa agctgaaggc tataggcgga agcggaagag
      781 cgggttcgag cgatgttatg gtatgtgaag cgtgcgaatt ttcaaatacg ttcttacacc
      841 tgttcccaaa catttctata atactgaaca ttgacgcaga ccaccttgac tttttcaaga
      901 cgatggataa cctgagagcg tctttcacca agttctgcga caatacaacg gatatccttg
      961 ttgtaaacgg tgatgatgaa aacactatga gagcggttaa agcatcgggc tttgacaagc
     1021 agatcatcac ttttggcaag agtgataaaa acagatatta ccccgaaaat atcgagcgta
     1081 tatccgattt tcagaccgat tttgacctta tggaaagcgg caggaagatt gagcgtatct
     1141 cgatacacgt tcccggcgcg cataatgttc tcaatgcggt tgccgcctgt gccgccgcac
     1201 tggcaacagg tgttacaccc gaaaacatca acaagggact aagcacattc tggggcgcag
     1261 ggagaagatt tgaacgtctt gcgactgtcg gcggtatcac agtggttgac gactacgcgc
     1321 accaccctgc cgaggttgcc gcaacactga aaacggcaaa agcaatgcag gacttcaagc
     1381 gcgtatgggc tgtgcatcag ccgttcacct attcgagaac atacacgctt atggacgact
     1441 ttgcaagcgc gcttgaaatt gcggataagg ttgtgctgac cgagataatg ggtagccgtg
     1501 agaagaacac atataacgtc tactctgccg acctcgccgc caaaattccc ggttgcaaat
     1561 ggtttccgac ctttgaagag gttgcacaat atgtttccga taacgcagaa agcggagata
     1621 tgatcatcac gctcggctgc ggcgatgtat ataag
//
LOCUS       DAWAOG010000497         1655 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9649_length_1655_cov_2.153125, whole genome shotgun sequence.
ACCESSION   DAWAOG010000497 DAWAOG010000000
VERSION     DAWAOG010000497.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9649_length_1655_cov_2.153125"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          425 a          399 c          394 g          437 t
ORIGIN      
        1 gtacaggtaa cagcacctat tatttttcgc tttcggattt tgaaaaacaa cttcctgcgg
       61 ggcaatttgc ccgctgtcat aacagctatc ttgtgaattt gaatgaggtg cgtgagatcg
      121 gtagaacgga gctttctctg caaagcggcg aaacgctccc ggtgggaaga gcctattaca
      181 aagcctttca aagcgcattt gtgcgatata taaatcaata acaaagaggt caccgcttat
      241 attcggcggt gacctcttaa tatttatttt ttaggcagct taagcgctgt ctgcacggtg
      301 aagatccgat cctccgaagt gaaaatatcc agtatgctgt ggtaacgcac ggcgacggtg
      361 ttcatcagct ttaagccgaa gccgtgggtt tcggcgtcat ttttggttgt gagatagttg
      421 ccgtccgcgt cctgtctgtg cttgccggaa tatgaattct cacactttac agcaagaaag
      481 ccgttctttg tttcaatttg aaagcggaca aaccgttttg cgtcggtaac ctccgcggtg
      541 gcttccaacg cattatcaag catattcatc aaaagctcgc ataaatcgct ttcggcaaca
      601 gtcagctgcg gcggaacaga aacgagcaca tcaaatctga tgtcggactg tgccgcacgg
      661 taagcggcgt cctgcaaaat cgtattgacc gtgaaatttt cggtaaactg cgtttgtgcc
      721 aaatgatcgt tttgctgttt tatatctcca atcagctgtc ccagcgtctt ataatcgccc
      781 ttctgataca gtgcgtcaag ggcgattatt ttatgcttca tttcatggcg aatggcggcg
      841 ctgtcggtca ccttgctttc tatcgcatga tagctgttca tgataagctc gttgcgcaga
      901 cggagcgttt tggcttccat ctgctgcgaa atgatcgaac gtgttacggc ataggcggag
      961 atgaccgcac atacggcgac tgtccagacg gtgatccaat acagaagccc tgaataatca
     1021 taaaactccg tgacatctga gatcagcctg ccgatatatt ttgaaaggcg gctgtttgtg
     1081 aaaaacgata ccatgtatgc gacagccaat gtaacggcac ttacggcggc ggaaacccca
     1141 aagtatttcc agaatttacg ttgtctgttc atcacaagat atactatcaa aagcacaagc
     1201 aacagaatgc ctatttcctg ccgattaagg aaatcgacca atccgtcatc gagaaaatga
     1261 tgtcccattc ctttggtgat ccattgcata gtaagcccca cagaggcgag gaaaagcggt
     1321 atcagactcc aatcagcatt tttgcgcgca attcccaaaa gaaacagacc tgcgatcatc
     1381 agcgatatga aagcggtcgc acctgctgga atgctgtaat aattcgcata ggcgtagctt
     1441 tcgatttctt ccgcatctac aggcacaaag cgtggaaaag gcgggaaaat aatgcttgtc
     1501 gtgtcggtca ccgtgccgat aacggtaagc ttacattcgg tattaagcgg cagcggaata
     1561 atcgcctggg cctgccctgc cgccccctcg ggtacaagtg tctcggactg ccacaccgct
     1621 ttttcgtcta tgctgaccgt cagattcatt cctgc
//
LOCUS       DAWAOG010000498         1653 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9674_length_1653_cov_2.168961, whole genome shotgun sequence.
ACCESSION   DAWAOG010000498 DAWAOG010000000
VERSION     DAWAOG010000498.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9674_length_1653_cov_2.168961"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1381..1437
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          361 a          393 c          324 g          518 t
ORIGIN      
        1 acagagtgta tctcaacgtc gccgtggtga gcttcaatag cgtggataac ctcggggctc
       61 tccttgtact tacggcagac atcgacacct atctgaacgt gcgagccttc aatctcgtgg
      121 ttaagagcct tgcctatatc atgtaaaaga cctgctcttc ttgcaagagt tgcgtcaacg
      181 ccgagttcac ttgccataat acctgcgaga tgagcgacct caatagaatg gttaagtaca
      241 ttctgacggt aagaggtacg gtagtaaaga cgaccgataa gtcttacaag ctcgtggtgc
      301 agaccgttga cgttcgtttc cataacggca cgttcgccct cctgcttgat cttgagttcg
      361 acctcacgtt ttgccttctc aaccgtttct tcaatcctcg tgggatggat acgtccgtct
      421 tgaataagct tttcaagcgc tattcttgct atctcacgtc ttacatgatc gtggcatgag
      481 agtgtgatag cctcgggagt atcgtcaatg ataaggtcaa cgcccgtgag ctgttcgagc
      541 gcacggatat tacgaccctc acgaccgatt attctgccct tcatttcatc gttaggcaga
      601 ggtactaccg atactgcaac ttcagatacc gtatctgcgg caagtctgga aatagcaagt
      661 gagatatact gtcttgcctt ttcgtcacat tcgtctttca ggcgctgttc gtaagctgtt
      721 atcttgaccg ccttttcatg tgaaagctcg ctgtcaagca tactgagcag atgctccttt
      781 gcctgatcca gagtaaagcc ggatatcttc tcaagtatat ccatctggct tttcttgata
      841 gattctgctt ccgcaagctt ttcttcagcc ttcttgttct tattctggag ctgttcttca
      901 agtcgttcga tcttatcgtt cttcttgtct aaattctcct ctttctgctg aagtcttctt
      961 tcagagcgtg taacctccgc tctgcgctcc ttgagttccc tgtctgtttc gtttttcagt
     1021 ctttgaattt ctttttcgtt gttctctttg agtttaaaga tctcatcttt agcctcaaca
     1081 agtgcggttt tctttgttgc ctgagctttt tcgttagctt cggatactat cctgctcgct
     1141 tccttttcgg cagattctat ctgcgcttct gccgtctttt ttctatgctc gatacccatt
     1201 ttgtacatta caaaaccgaa tatcgccgct ccgactacga atgccgccgc acagagtgta
     1261 actgcaagcc acacttccat gggttagcgt tcctcctttt cagaattacc gtgtcacgcc
     1321 gccgcaaaac tgcatatgtg cctgcacggc tgattttaca tttcaacatt taattatata
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgt
     1441 caagggcttt ttataaaaaa acactaaata attttggaaa atggtgaaaa tctattgaca
     1501 atatttttct gctatgatat aataatcgaa ctgtgaaatc gttattttgt gacacagtgc
     1561 gctttcacaa agacaatatc agccgatgtg atggaattgg cagacgtgac ggactcaaaa
     1621 tccacaacgc cgtttcatac caccttggcg taa
//
LOCUS       DAWAOG010000499         1652 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9685_length_1652_cov_1.634941, whole genome shotgun sequence.
ACCESSION   DAWAOG010000499 DAWAOG010000000
VERSION     DAWAOG010000499.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9685_length_1652_cov_1.634941"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    608..696
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1058..1067
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          428 a          375 c          329 g          421 t
ORIGIN      
        1 aggagtattt cccatgaaaa aactgatctt acttctgctc tgcctgagcc ttgtcctgtt
       61 taagtacatg gacaggtgct tctgtaatgt tctttaacac cgacatatgc gggaaaaggt
      121 tgaagttctg gaacaccagc ccgattttca gcctgatatc gtggagcgtc ttattgtccg
      181 catatacagc cttgccgttt tcatcatttt tagtcatagt gatgccgcat atttcgcact
      241 caccgctgtc tatcttctca aactggttga tacagcgcag gagcgttgat tttccgcttc
      301 ccgacggacc tattatagag ataacctcgc cctgttccac attgaatgaa atatcgttca
      361 atacatcaag cttgccaaag ctcttggata tattctttac actgagtatc gacatattga
      421 cagctcctta tctgtagtag ttaagcttct tttcgataaa gccgaacagt gccgtgagca
      481 cagtagccat tacgaaatag aacacaccgg caataaacag cggcacaagc gagctgtagt
      541 tcatcatctg ctgtttagcc gcaagcataa tttccgagta agcaactaca tttgcaagtg
      601 acgtatcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcac gatgcgcttt atgacctgcg
      721 gaagtataat tctgaagaat gtctgcgcct ttgtcattcc cagagccgcc gccgcctcat
      781 actgacctct cggtatcgac tcgataccgc ctctgaatat ctcggcaaaa tacgctgcat
      841 agttcagcac gaatgcgata attaccattg tgaacataaa tccctctgct gtgcccatcc
      901 gcaagcgctt gtggataacg ataccttcat cggaagtgtc tacccggtat gaggagagca
      961 ggtcggcgcc gtagttgtgg cggacagtca gcgagcccgg aaaacaagcc tttccgtaag
     1021 gggaaagcat cagacactcg ccgtccacgt ccgatatnnn nnnnnnngat ctacaccgct
     1081 cagaagattt tccataaatc cgcccgagcc cttggttgcc gtctttaaga aaggcagggc
     1141 ataataaaca acgatgatct gaagcataag cggagtgccg cgcattatga gtatgtagag
     1201 gtttgtaagc cacgatatcg gtttaaatct gcacatcttc agcgccgcaa cgacaagtcc
     1261 gagcggtatc gaaaacagca gagtaaatcc gaaaatttta agtgtgggga taaggttctc
     1321 gaggagtatc cccgtaacct tcattatctg ttcaccggtc atatttaaac atcttcctta
     1381 tttttaattc atcacggtaa agcgataatt cgctattacg gcagtatatt agtattgtac
     1441 cataagtaaa gggggatttg caagtaaaag atgaaaaaca gcgtcatatt attttattgc
     1501 agtcgatgct gtagctttac taaacagcca catcgctcaa aacagtttcc gcacacccaa
     1561 ggtgttgata ttttcgcgag tgtatcagtg caactgtact gtcaccgaaa tttccaaaaa
     1621 acgcactacc ctaagatatc tgtacagtcc cc
//
LOCUS       DAWAOG010000500         1644 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9742_length_1644_cov_2.168030, whole genome shotgun sequence.
ACCESSION   DAWAOG010000500 DAWAOG010000000
VERSION     DAWAOG010000500.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9742_length_1644_cov_2.168030"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    977..986
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          486 a          342 c          342 g          464 t
ORIGIN      
        1 ctgtgttaca gccttttata ttggctctta ttaccgcata gtccgaaaga acaccgtttt
       61 ccttgacatc ttcaatgaac gcacgaaatc ctttcgagtt cggtatgccg ctgtcgcgat
      121 caatgttgtc gatcagcttt ccgacatgaa tgctgagagt gcaggtgcaa attatagcaa
      181 gaacatgggt aacgtgctca aaaagctttt cctgctccgg gctgagcgtt gcaaaatcaa
      241 tatcggtata aaattcatat gcgtatctgc aactatcgtt ctcacattcg tattttttgt
      301 tatagagagg aaaaatctct gcgtctttat acaccagagt ttcgcttatc tgcccaccgt
      361 aatcaagtgt gcgcaggatt ctggatatgc gtaatgcttt tgccacaggt acaaactcat
      421 ctatctgtcg caggtcgctg taccgttttg tagctacggc atcaatagtt tcagacaggt
      481 gctttctgac aacgtcgatc atattcctga ctccttcttc tgttttctcg atatctttat
      541 atattttatc acatttccga gtcgttagca agcaaaaaag cggacaaaag gccaaaaaaa
      601 gttcacaatt tcaaaataag agcagagtag acaaaaccgc ttcgcaaaag ctgtgcatta
      661 tcacaaacag ccgtccgttt ataatcgttt acagcgctgt tttatattgt atttgtgcat
      721 attttaagta aaatttgtat tttagcttat aaactaatat cggctgtttt gtcacaaaac
      781 aagccgcaaa tgatgaattg atgcgataaa aaggtctttt gtcgaaaaaa tcagaaaata
      841 ccacttgaaa acctactaaa aatatggtat tatagtattt agaagatcac ggctcacgaa
      901 cggctcagat aaaataaagg tcaagaaagg actataacat gaaaatatct acaaaaggac
      961 gttatgtatt gagaatnnnn nnnnnnctca ttgatcttgc ggagcatcag ggtgacggtt
     1021 atatcccgct gaaagaaata gcgcaacgcc agaatatatc aaaaaaatat cttgagcaga
     1081 tagtaccgat gcttaacaaa tcggatatcc tgcgcaccaa ccgcggattt cagggcggct
     1141 acagacttgc aaagtcaccc gaaaagtaca ctgtgggaga tattttaagg cttaccgagg
     1201 gttcgcttgc gccggttgcg tgccttgaca gcggagtcgt cgaatgcgac aggagcgagc
     1261 attgtgctac attatcactc tggcagggtc tgtacaaggt cataaacgaa tatcttgaca
     1321 gtgttacttt acaggatctt atagataatg ataaggaaaa cgccgccgat gactatgtga
     1381 tataagtgtc ggcgttaatc agaataatat ggaacagccg cggtcatttg ccgcggctgc
     1441 tattatacac aaaaatcccc cgcagaaacc tgcgggggat aaattgctta tttacgctta
     1501 ttgcttaaag ccgaaattac tttctcttct tagcaacgat aacagcgcct gtagcgagaa
     1561 ctgcaacgcc tgctactact gcaacgctct caacacctgt gtttgtgctg ggctttgtag
     1621 agtcgcctgt tgtaggagca tctg
//
LOCUS       DAWAOG010000501         1642 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9755_length_1642_cov_2.378072, whole genome shotgun sequence.
ACCESSION   DAWAOG010000501 DAWAOG010000000
VERSION     DAWAOG010000501.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9755_length_1642_cov_2.378072"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          558 a          334 c          279 g          471 t
ORIGIN      
        1 ttactaaagc aatataatag gagcaaacca tacaaaaaag tgagccttac gcgctatttt
       61 tagcgcttcg actcactttt tcttttccgg gttgtgccac aaccacgtga acattcaaaa
      121 aatatattta tttgtcgtta tccttcgact ttctgttact tataacagag cctgtcacta
      181 ttccgccgac aaggactacc gcacccacag tgctgagcac tatccaaccg gggaacgcat
      241 tgctttccgt tgtctgctct gcaataagtc cattcttagt catttccgct cttgcattac
      301 tgccgttgtc aacgggcgat tgagcggacg cataaatgct gactgttagt aaagtagttg
      361 cgagagcaac tattgaagtg atcgttttta atagctttgt cataatttcc agtcctttca
      421 tagttttatg ttttattgtt atatagtaag gtgtattttc tttcctgatt ttagtatatc
      481 acataaaaac tgcaagtcaa tatttatgac aaaactgtaa ctattttgtt accggtttgt
      541 aacctttttc ggtcgttcgg caggcgcatc aactgccgaa catttgcctg cggacaaatg
      601 agggctttca ttatacagaa aagcggctga cagccctaaa aggcatcagc cgcataacag
      661 atataattgt ataacccata tatacataat taatcaagca atacagttaa atcataaccg
      721 aagcatatca gacacactgc tatgtacaga atcggatttg aaataatgac aattaaccgt
      781 tctaaacccg ttctgcactt tcgcagaaaa taaaatgttg ataaaataac aaacaagaaa
      841 atagcacacc atgcataata gatagaatcc aacagttcgt tcagaaggca agcaataaag
      901 ataaaaacta aagaaatcga cgacacgata gccctgtctt taagataaaa gcttgtatcg
      961 gttttggcaa tcagcgtaat tactatcgga agaaatccgc caagtaaagc gctgaacaaa
     1021 cagccgagcc ttaaagcgtt taattctttg ccaatagaaa aaatgacaaa agaagttccg
     1081 aatatcagac atattaatat cgggaaaagc aacgaaccga taatctttct gcttgtaaaa
     1141 ttatgcttca taaaacctcc gataccgaaa cactacagtt tagttttttt gcagactaat
     1201 cttgttaatt ttgctagagc gaaagacggc aattgtacaa taagcatttc taatactata
     1261 attaccgcat cggtaaaaac atcgggatta aaaaattggg aacttcccct atggagatag
     1321 tataataaat acaccccctc gttttcaaat atcaagacaa acagaaatgt aataaccgca
     1381 ccgcagaata ttgcgtatac gggcaaatca aaacaaagaa ccgccagata cgttataagt
     1441 attaccgcaa cgaatgagca agctatttgg atgtttcgga tgtcatcaaa actgctgttc
     1501 ataacgtcaa taatagaatg gataagtata tgcattagca acaccaatac ttgcatcccg
     1561 atacctaaac ttatattttt tgcaatcttc atttaatcct cctgtctgtc gaggttgaat
     1621 cagatagtat aacacctgta ag
//
LOCUS       DAWAOG010000502         1637 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9807_length_1637_cov_1.746523, whole genome shotgun sequence.
ACCESSION   DAWAOG010000502 DAWAOG010000000
VERSION     DAWAOG010000502.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9807_length_1637_cov_1.746523"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1374..1422
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          455 a          308 c          376 g          449 t
ORIGIN      
        1 gatttattcc gaaaaatgaa ctgcaatatt ctttcaggga atttgcagaa tatctcggca
       61 aatgccgttt cagagattgt gttcatctta aagagagcgg ctgtgccgta accgaagcac
      121 tgagcgaggg taaaatagag cagtcccgct atgacagcta tgcaaggatg tgcgaggaag
      181 cggataaact caacgaatgg gagtttaaga ataagtaatg aaaacctgtt atattatatg
      241 cggcgggcct gaaccgtgca aaaccgtaag gataccgaag gatacatttg ttatatgtgc
      301 ggacagtggc tatgacaagg catttgccgc cggaattacg cccgacctta ttctcgggga
      361 ctttgacagc atagaaagca agctaccgca gaatatcgaa atactgcgtt cgccgacgca
      421 taaggatgat accgacacta tgcttgccgt aagaactgca cttagcagag gatacgacag
      481 catagcgctt gtcagcgcct gtggcggaag aactgaccat acgcttgcga atatcgcaac
      541 gctgcttttt atacgtgagc atggagcaaa tgcattcata aaaggcgaca cgacagatgt
      601 ttacattctt gaagatgaga agataacatt gctaccagat ttgtcaagat atctgtcggt
      661 gtttgctgta agcgataaag caaccgtacc cattaccggg gcagagtatc cgctgaaaga
      721 ttatgttatg gagcgcagct ttccgatagg tgtcagcaat gagtttattg ctgatacaga
      781 ttgtacggta gagataaaaa gcggactggc agttgtaatg actgtaaaaa gataagtaac
      841 aaaatacctc ccgacggaat aaagtgtagt aactttaggt attgtttatg atgagaaggc
      901 gcagatcggg acggtcaaaa tttcctgctt tcatactcgg tcttgcgggt attcttatct
      961 gcgttacctg tatgtcggct gaaacactgc ttgttatact gtccctgctg cttattgcgc
     1021 tcggagtatg gctgttattt ttctgctgaa agagaggtac atattatgaa aatcgtagtt
     1081 tgcaaaacac caagaatact ggcgggcttt ctaagactgt ttttcggtat aaaaaagaca
     1141 gaaagctaaa gtacagccct cgtccgaatt tcagacgagg gctgacttta tgccgatgag
     1201 caaacattat cgatattttc cgttattgat atttttcata aactgtctgc gtgtcatctg
     1261 tcctgtgctg actttccacg agtcctcctg ctcaagcttc cagttgttgt gatatgatgc
     1321 gggaatctgc ggttcgcaat ttttccatat tccatatata atcaggccga taannnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttctctgg tgttgcttca
     1441 gcgtttttcg ttacttgtat aatatcatat gatgtgtacg ataaaaagga caatatggtg
     1501 atattaagct cctgtggtaa tttgtgtact ataagtttta gcggaaacaa tttaaaaggg
     1561 cagtgccgca aagcactgcc cttaagccgt cgtttaatat gctcagtcgt tgaacttagc
     1621 cataagctcc ttggtaa
//
LOCUS       DAWAOG010000503         1633 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9835_length_1633_cov_2.757288, whole genome shotgun sequence.
ACCESSION   DAWAOG010000503 DAWAOG010000000
VERSION     DAWAOG010000503.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9835_length_1633_cov_2.757288"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1119..1128
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          479 a          351 c          400 g          393 t
ORIGIN      
        1 cttaaggacg ctgtatcgga ggctatgcgt gagtggacaa agagaattga cgatacgcat
       61 tactgcttag gctctgtaat gggaccgcac ccctttccca ctatcgtccg tgactttcag
      121 gcggtaattt caagggagat aaagcaacag cttatggaaa aagagggcag actgcccgat
      181 gctgttcttg cgtgtgtcgg cggcggctca aatgcgatag gtacattcta tcacttcata
      241 gaggacaaga gcgtaagact tatcggatgc gaggcggcag gaagaggtat agataccttt
      301 gaaacagcgg ctacggtaaa cacaggctcg gttggtatat tccatggtat gaagtcgtat
      361 ttctgccagg ataagtacgg tcagatagct cccgtttact cgatctcggc aggacttgac
      421 tatcccggta ttggtcctga gcacgcatat ctgcatgata taggcagagc ggaatatgta
      481 cctgttactg acaatgaagc ggtaaacgca tttgagtacc tttcaaagac cgaaggaata
      541 atccccgcaa tagagtcagc ccacgctgta gcccacgcta taaagcttgc tccgacaatg
      601 gataaggaca agataatagt tataacgatt tccggcagag gcgacaagga ctgcgccgca
      661 atagcacgtt acagaggaga ggatatacat gactgagata gcaaaagcat ttgaaaacaa
      721 aaaggcattc attcccttta taacctgcgg cgatcccgac cttgaaacca ccgcaaagat
      781 agtaagagaa gcggtcgcta acagagcgga tttaatagag ctcggcattc cgttttccga
      841 tccgacagcg gaaggtcctg taatacaggg ggctaatatc cgtgcgttaa agggtggagt
      901 tacgacagac aaggttttcg atttagtcag agaactgaga aaagacgttg ctatccctat
      961 ggtgtttatg acctatgcta acgttgtatt ctcatacggt ggggacaagt tcatttctac
     1021 ctgtaaggag atcggcataa acggacttat tcttcccgac ctgccttacg aagaaaagga
     1081 agaatttctg ccgctgtgca ggaagtacag cgttgaccnn nnnnnnnncc tcggaaaacc
     1141 gcattgcaat gattgcacag gaagcggacg gatttatcta tcttgtatca agccttggcg
     1201 ttaccggcac aagaagcgag ataaacaccg accttaagtc tattgtcgat gtgataagac
     1261 agaacagctc agtcccatgt gctataggct tcggcatctc aacgcccgag caggcaaaga
     1321 aaatggctga catcgcagac ggcgcaattg tcggctcggc tatcatcaag ataatcgagc
     1381 agtacggcaa ggacgctccg aggtatgtcg gcgagtatgt aaagagcatg aaggacgcta
     1441 taagataaga attcttaata tatctacggg ctgtgaaaat cagcccgtat tttttcactt
     1501 ttttactttt ttcatggaca aaacgtgctt ctgtagtgta ttatatataa caagagcaat
     1561 ccaccatact gttttgtatt tacgaaagga gcattattat gaaatcatcc gaaaaacgca
     1621 ttaaaaccct acc
//
LOCUS       DAWAOG010000504         1629 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9874_length_1629_cov_2.106734, whole genome shotgun sequence.
ACCESSION   DAWAOG010000504 DAWAOG010000000
VERSION     DAWAOG010000504.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9874_length_1629_cov_2.106734"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          411 a          395 c          341 g          482 t
ORIGIN      
        1 cttttctgaa tgtagtcgtg ctgttatttg ttattttacc aacgcgtacc cacttgttgc
       61 cctgatacat ctcaatgata tatccgtcag cggaagcatt cttagtccag tttacgcgga
      121 gcgcgtcagc cgctctgccg ccgagctttg cacctttcat tacagacgga tttgtccttg
      181 ccgcaacagt cgcgctgtaa tcactgtaaa gcgccgtact gccgctcatt ttgtatgctt
      241 tgaccctgaa cttataggca gttcccgcct taagaccgct aaccttataa gtgatagtac
      301 tgttcccggt gatctttgtg actcttgacc acttgccgtc tttgtatatc tcaatgatat
      361 aaccgtcaga gcttgtattt atgccccatg tgagtgttat tgaagtgtcg gttacttttc
      421 ctgccgtcac tccccttaca gctgtcggag ctgtacttac gacctcaaac ttaaagccgt
      481 tttcgcttgc atattcctgt gcggctgagt tctttgcgcc gcgcagaaca aatccgtcca
      541 ccttctgatc tgctgtcacg tttatcttgt gataaaatcc gaaagctcta tcgcctataa
      601 gctttaccga tgcaggaacg gtaactgatc ttaacgcagt acagccgtaa aacgcataat
      661 catctaccga ataaagttta gcaggcagtg ttacgcttgt aagcttttca gccttgtaga
      721 aagcataggg tctgatataa tatgtcttat ccgaaacctt gaaggaagtg cctgctttct
      781 ttgcgggata aagaataagc ttatccatat cctttgtgta taacgcgccg ttttcactgc
      841 aaaaagccgt attgccggaa gcaaccgtta ccgatgcaat gtttccgcaa cggtaaaacg
      901 cattctcata aagtaatgtc aggcttgccg gaatgttgat cctactgagc gatgcgcagt
      961 ttgcaaatgc actgtcaccg atagaaacga ccttggacgg tatagtgata ttgtcgagca
     1021 gatcgcagta ataaaaagca ttatccaaaa tcttcgtaag atttgcgggt aaatttacac
     1081 tgctgagcga cagacactgt aaaaatgcac cgctaccgat agaagtaaca gttgcaggca
     1141 aagtcacctt ctgcaaatac atactgttgg cgaaagtctg atcttcgata gtcttaacac
     1201 catcgggaac tgctacgctc ttaagcgact tgcaggcata gaaagcctgt tttccaagat
     1261 agtcaagctt cttcggcaag ctgaccgtgc ttaatgcgtc acagcagtag aacgcctttt
     1321 cctcaattct cgtaacactg tcaggaactg ttacctttgt gagcttgggg cagtaagcaa
     1381 aagcgctctt cggaattgtc gtgatcgttg acggcaattt caccgatgtg aggttatcgc
     1441 aagtactgaa cacataatcg cccatctctg ttattccatc ggcaattgta acagtcacaa
     1501 ttttcgagtt gccattgaag atatttttta tcttgcctac atttctgccg ccgatcttcg
     1561 cgggaacatc ggctttaaca tcatcaccga tatatttcgt gatagtcgca gtcccgtcat
     1621 cttcaagta
//
LOCUS       DAWAOG010000505         1627 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9887_length_1627_cov_2.290712, whole genome shotgun sequence.
ACCESSION   DAWAOG010000505 DAWAOG010000000
VERSION     DAWAOG010000505.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9887_length_1627_cov_2.290712"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          456 a          383 c          410 g          378 t
ORIGIN      
        1 agcctgcaaa gcactcaaaa aactgggcta cgaaatagtg cttgtaaact ccaacccggc
       61 aactattatg acagaccccg atgttgcgga tatcacctac attgaaccgc tcaacctcga
      121 cagactgacg cagatcatcg agcaggaacg tcccgatgcg cttctgccta acctcggcgg
      181 tcagtccggt cttaatctct gctcagagct ggacaaggca ggcgtgctga aaaagtacaa
      241 cattcaggtt atcggcgttc aggttgacgc tatcgaacgt ggtgaagacc gtatcgagtt
      301 caagaatgca atgaaggatc tcggtatcgg tatgccgaga agtgaagttg cttacacggt
      361 tgacgaagct gtaaagatcg cagaggagct taagtaccct gtcgttcttc gtcctgccta
      421 cacaatgggc ggcgcaggcg gcggtatggt atacaacgtc gatgaactca aggttgtgtg
      481 tgaaagaggt ttacaggctt cactcgtaaa tcaggttctg gttgaagaat gtatcttcgg
      541 ctgggaagag ctagagcttg aagttgtccg cgatgccgat aacaacaaga tcaccgtttg
      601 ctttatcgaa aacatcgacc ccatcggcgt gcatacaggt gactcattct gctccgctcc
      661 tatgctgact attcccgaag atgttcagaa agagcttcag gaaatgtctt accgcattat
      721 cgaatccata gaggtcatcg gcggctgcaa ctgccagttt gcccgtgacc ccgaaacagg
      781 acgtattgtt gttatcgaaa taaaccccag aacatcacgt tcttccgctc tcgcatcaaa
      841 ggctacaggc ttccctatcg cctttgtttc agctcttctc gcctgcggtc ttaccctcaa
      901 ggatatccct tgcggcaagt atggcacact tgataagtac taccccgacg gagattacat
      961 tgtgatcaag ttcgcacgtt gggcatttga aaagttcaag ggtgctgagg acaagctcgg
     1021 tacacagatg agagctgtcg gcgaagtcat gagcatcggc aagacctata aagaggcttt
     1081 ccagaaggct atacgttcgc ttgaaaagga cagatacggt ctcggctttg caaagaactt
     1141 ccacgatatg actaaggaag agcttttagc acagctcaga taccccacaa gcgaaaggca
     1201 gtttgtaata tatgaagctc tgcgtaaggg cgcaactatc gaagagatcc atgctcttac
     1261 aaagatcaag gactggttct tacagcagat gaaggagctt gttgacgaag aagaagctct
     1321 gcttaaagca aagggcgcag ttcccgaaaa ggctgtactg aagcaggcaa agcttgacgg
     1381 cttctcagac cgttatttaa gctcactgct tgaagtaaca gaagaagaaa taagaaatgc
     1441 acgtatcggt ttcggcataa aggaagcatg ggagggtatt catgtaagcg gtaccgaaaa
     1501 tgcggcttac tactactcca cctatcacct tgacgaggat aagagccctg tcagcgacaa
     1561 gcctaagata atgattttag gcggcggacc caacagaata ggtcagggta tcgagtttga
     1621 ctactgc
//
LOCUS       DAWAOG010000506         1624 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9921_length_1624_cov_1.838113, whole genome shotgun sequence.
ACCESSION   DAWAOG010000506 DAWAOG010000000
VERSION     DAWAOG010000506.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9921_length_1624_cov_1.838113"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    370..379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    774..783
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          386 a          392 c          323 g          503 t
ORIGIN      
        1 gagctgttga tatcccacgc aagctccaac ttatcgctgc tttttgtact tactctgaaa
       61 ttctttacag cctcgagcgt aagcatctct gtatatgtgt cattatagct gtcaccgcac
      121 cttgaacact cgtgatgagt atagccctta ctctcatatg tgggagctat aacggtgctt
      181 acataatcgt gaccgagcgc gtcgatttct tctttttcac ttgttccgca accgccaagg
      241 caatatctta tacgctcacc cggaactgta caagtcggtt ttttgaccgt ttcccattcg
      301 ccgaatgtat gatcacctgt tgtcgggata ctcataatat ccgttctggc atcacagcgt
      361 gtgcagtgan nnnnnnnnnt cagcaggtct gtctactgtc cactcggcag aataatcgtg
      421 accgagcttt tcgataatgc cgttttctac agctccgcat ctcttgcatt ctctgtgctt
      481 tgtaccgtct ttcgtgcagg tagcggctgt atccgttatc cattcaccgt atgaatgtac
      541 tgtgcatttt gtatcaacag cataagcctt gatcataagg ttatttttat tattattttt
      601 acaatcatcc cagaagctgt cgggatcata tgtataatag ctttcgccct tgttggcatt
      661 gagcgtgtat gtaagcccgt tcatattgta gccgaattca ctgtaaattc ttgcagaacc
      721 gctgttattt gttatttttg caacaatagc aaaagtatcg cccttctgta cttnnnnnnn
      781 nnntaacgaa taatatcctt catatccaac attttcctta gtcgccgttg cagcttttgt
      841 acctgatgtc ggatttgttt tgtctttaag accggtatat acgctgagtt ctatatccat
      901 accctttgat gtatatacgg cagcacccgt tatattggaa gcatcttcgg cagtaaatac
      961 atttgcaccg tacattgtcg acagcccgaa gccaaaccag ctggaagaat tgccgccgtc
     1021 atagttgtaa agcgtatcgt aataatcatc tgcaccgtct actgtataat cgtatgcaac
     1081 accgctggac gctatcgact tgtcatagta tgacagccag aagtaaccgt catctctgct
     1141 gtcatctccc cagctgttct ttatcagcca tgcgccgtta cccgcgggag cggtcttgaa
     1201 tgcagacgca ggtatactgt cgtcccagcc tactaccgtg accgcatgat tagatgtata
     1261 ttcctcaggg caatagtagt aatagctgct gccgtcatca ccgaattttc cgtagctggc
     1321 agcatcgcaa tagctgagcg aagctgcacc gtgtgtcaga ataagctctt ttagctgatc
     1381 gttgctgtta ttctgattga tatcggcaac atacatacct gtaagctttg ctgcgcctga
     1441 atttctcagt tcataatact cagactctgt aaacgatttg tttactatac ccggagtacc
     1501 atatgacgaa tacggatact gcgagttttc ataatacgga cctatgccgt tcataacagc
     1561 aagtccggca ttccatacgt taccgccgag atctaaatag tcggtagcag atgaagccgt
     1621 atat
//
LOCUS       DAWAOG010000507         1624 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9918_length_1624_cov_2.573614, whole genome shotgun sequence.
ACCESSION   DAWAOG010000507 DAWAOG010000000
VERSION     DAWAOG010000507.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9918_length_1624_cov_2.573614"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          460 a          351 c          433 g          380 t
ORIGIN      
        1 gaacatgggc aagagaacgc catgtacagc gtttccctat acgttttggc aagcagtcga
       61 tcgactccaa cagaggtgct acgagccacg ctcacaacaa cttctttgcg ctgtgcgacc
      121 ataccgcaac agaggacttc ggcgaagcat acggctttgc tctggtgtat tcgggttcgt
      181 tcttaggaat ggtagaggta ggtcagtatg aaaagacaag ggcgctgctc ggtataaatc
      241 cctatgactt ctcgtggcac ttagagccgg gagaggattt tcaggcaccg gaagtcatca
      301 tgacctattc cggcgagggc ttaggtcaga tgtcgagaaa cttccacgat gttatgagaa
      361 acaaccttat aagaggaaag tggaaggata tgcgccgtcc tatacttata aataactggg
      421 aagctaccta cttcaacttc gacacggaca agctccttga tatcgcaaga gaagcaaaga
      481 aagccggaat tgaaatgctt gttatggatg acggctggtt cggtcacaga aacgatgata
      541 aatcttccct cggcgactgg tttgtgaacg aggataagat aaagggcggt cttaagcacc
      601 ttgtagacga ggtaaacaag atagggctta agttcggtat atggttcgag cctgagatga
      661 taagtcctga cagcaagctg tacgcggagc atcaggactg gtgcatacac attcccggaa
      721 gacaccgcac gaccgtcaga agtcagcttg tactcgactt ctcaagaaaa gaagtgcgcg
      781 actatatcta tgagcagatg aaggctatcc tttcaagcgc aaacatagaa tatgtaaagt
      841 gggatatgaa ccgacagctg agcgaggtcg gcaacgaggt attccctccc gaaagacagc
      901 gtgagatatg gcacagatat gtcctcggag tatatgaaat gcaggaaagg ttgctgactg
      961 atttccccga cctgctcctt gaaaactgcg cgggcggcgg ctcacgcttt gacgcaggta
     1021 tgctgtacta ctctccccag atatggacaa gcgatgatac agatgctata gaaagactta
     1081 agatacagta cggcacttcc atgtgctacc cctgctcctg cttcggcgct cacgtttcgg
     1141 acagcccgaa ccactgtgta ggcagaatta ctccgtttga aacgagagga cacgttgcga
     1201 tgtcgggtac gttcggctat gagcttgacg ttacgaagat ttcggacgat gacaagtatg
     1261 cgataaagga acagattgcg gagtttaaca agtataatcc gcttgtgaga acaggcgatt
     1321 attacagaat aggtgatccg ttcgcaaacg accgctttga cgcatggcag tttgtcgcaa
     1381 aggacaagag cgaaacgctt ctcgaatatg tacaggttct caaagaaccg agcgtgttcc
     1441 tgcacagagt aaagctgaaa gggcttgaaa agggctgtta ttacaggcac gagcagacag
     1501 gaagagttta ttcggcagag cttctgatga acagcggatt tgacattccg tcactttggg
     1561 gcgatttcag atcatttatc gcgcattttg taagggtgaa gtaagattgt ttatcctatg
     1621 atta
//
LOCUS       DAWAOG010000508         1622 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9934_length_1622_cov_2.190172, whole genome shotgun sequence.
ACCESSION   DAWAOG010000508 DAWAOG010000000
VERSION     DAWAOG010000508.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9934_length_1622_cov_2.190172"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    684..693
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          379 a          371 c          339 g          523 t
ORIGIN      
        1 aatggtatgc agatcagaac aatcggcggc gtttcggtaa tccataaaaa gcgttcccgc
       61 tatattccaa gtaccgtcaa gcagtcggcg aagataatac tcgttatcct tgctctgtac
      121 tatcggatag ttgatttcgg tatccgggca atagatccaa gcaataatgt ccttgttttc
      181 ttcctgtaaa cgctcaaagt ctaccgtaat tggtgcagtt tcaggaacag gctttatcgc
      241 agattctgtt tgtccattct ccgtttgctg tgtttgtggt gcggtttcta ccgctttatc
      301 aatcagcaca tcattctgtc tttgactgtc ctgtgtttct ttccaataat cataaatgtg
      361 atatgcagat aaagaaaaaa cagttataca tacagataaa atcaggattt tccatacctt
      421 tttaatagcg taacaccgtc cttatttgtc tgctttttta tcatgcacct tagtcagctc
      481 ataagtgccg ccgattgttc cttcgccgcc gatgagggtg agagtatcgc ctttgacact
      541 aaactcatag gttgcatcat gtgccgactc gttcacaaag tcgatatata gctgattgtc
      601 cttgatttca taggtaaaat cataatctgc aagggtgagg agcattgcac cgctgccttt
      661 gccgtcaaat tcataggtag tgtnnnnnnn nnngtgttgc tgtctaaatc ccaagtgcct
      721 gcaagtgcgt ctgtcgcacc cgaacagcct tttacaatca aaataatggc aacaataatt
      781 acaatcagca ggacagaagc accgccgatc tgcatacggc gcttgttctg ttttttcttt
      841 ttcattctgt ttctataagc gattcgttcc gactgagtta aaggtctttc cgagcgcctt
      901 tgctcgccgg agctgatagg ttgatagtct tgcatcgctt tctccaatct aagcaggaag
      961 gcggtagcgg gaatccccgt caccgccctt tgcttaaagg tctattgctg acttattcag
     1021 cgtgttcttt tttctttttg ttgtaaactg ttattcccgt aacgccagct accgaaacaa
     1081 acagcaatgc aaaccaaagg aatatattgc tgttatctcc ggtctgcgga gatttttcgc
     1141 tgccattggt atttccgggc tttgttgtgt cattaggctt tgttgtgtct ttcggagcag
     1201 tcgttgttcc tgttgcagga atttcaactg ccgggcgttt gtaaccgcag gtcttgcatt
     1261 cttcatgctt ggaacccttt ttggttgcgg tagcttcctt gtcaacaacc cacttgaagc
     1321 tgtgtgctgc ttcatccttc ttatctccac agttgcactc atgccagtga ttatctgcat
     1381 tggatttcca ttcagtacca tagtcatgaa tatgttcact acctgctgtt ctaactgtca
     1441 ggttagcgtt tgcctcaccg tctgtgtaga caaaggtgat tgtatgactt ccgtctgtca
     1501 gcgtaccgag ataacttgct ttcagggtaa gaacggtaga accggaaaca gcggtataat
     1561 tgctggaatc aacaactgag ccatccacct taattcctgt gaattttgca aaatcaccat
     1621 tg
//
LOCUS       DAWAOG010000509         1620 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9946_length_1620_cov_2.104792, whole genome shotgun sequence.
ACCESSION   DAWAOG010000509 DAWAOG010000000
VERSION     DAWAOG010000509.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9946_length_1620_cov_2.104792"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    993..1002
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          503 a          311 c          379 g          417 t
ORIGIN      
        1 tcgttattat cgcaaggaga gcgaatatta tgtcacagat tatagcagta acagcaggta
       61 aaggcggaac gggcaaatcg accacttcag ccaacgttgc ccgcggatta gccgctttag
      121 gcaagagaac tcttctggtt gagctcgatt tcggattaag atgtcttgat ataatgcttg
      181 gcattaaaga taaggttaag cacgatatag gtgaatatct tgaaggtaag attgatattc
      241 ttacagcgac aactaaggtt gaaactgtag aaaatctata tctggtttgt gcaacacgaa
      301 acccgtttat ggatatcaac cctgaaaaga tcatgggcgt atgcgaagag atgcgtcagc
      361 attttgacta tataataata gacacagccg gtgtcggaag ttcggtgttc agcgttatca
      421 aggcggctga gctgattctt atggtaacaa ctcctgatac agtatgcgtc cgcgatggtg
      481 cgatactgtc tgatttcctg tatgtaaaga attgtacaaa tcagcgactt atcatcaata
      541 aggttagcca gaatttcaag gacgaagaga tactttatga ccttgacgaa gtaatggact
      601 ctgttggtat acagctctta ggtgttgtgc cggaggacaa taatatcaag gtttgcggcg
      661 caaaaggact tcctttgccg cctacttgtc ccggcgcaaa agcatatgcg gctattgcaa
      721 gaagaatact tggggaagat gtaccgctta ctatcaatat caactaaata gtttatgtta
      781 agtgtatacg gtataaagca gaggaaaaaa gaggtttaat atgaatattg cactaattgc
      841 tcacgattcc aaaaaagaac tgatggttca gttctgtatt gcatattgcg gcgttctcag
      901 ccgacataac ctatgcgcta ccggaactac cggtaagctt gtatcagagg caacggggct
      961 tcatattcaa cgttatctca gcggatctca agnnnnnnnn nngatcagca gatagctgca
     1021 agaatttcat gtaatgagat agatgtactt attttcttca gagatccttt gaacgctaaa
     1081 tcgtacgagc ccaacgaaat gaatcttttg cgtctttgcg acgttcataa tattccgctt
     1141 gctacgaata tagctacagc agaggcgctg atacacgctc ttgagcgcgg tgacctcgac
     1201 tggcgagaaa ttgtcagagg ataaatgctt atgcaggcgt acttatgtac gcctttttta
     1261 tgttaaaaat cgaaaggaac aaatcagatg gagctagtaa ctaaaagacc cagaggaaca
     1321 gaagacaaac tgccaaaaga aatatataag tggcacactg tcgagaaaat cgcaagaaat
     1381 gttgccgaag catacggctg caaggaaata cgaacaccta catttgagca cacggagctt
     1441 tttcagcgtg gtgtaggcgg cacaacagat gttgtacaga aggaaatgta cacattcaac
     1501 gataaaaagg gcagaagtat cactcttaag cccgagggca cggcaggtgc tgtccgtgcg
     1561 gctatagaaa acggactgct caatgatgcg cttccgctta aaacatatta ttttacacgc
//
LOCUS       DAWAOG010000510         1615 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_9990_length_1615_cov_1.758974, whole genome shotgun sequence.
ACCESSION   DAWAOG010000510 DAWAOG010000000
VERSION     DAWAOG010000510.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9990_length_1615_cov_1.758974"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          456 a          365 c          351 g          443 t
ORIGIN      
        1 ttatattatc attgcattca atgtcggacg ctttgttttc aagtatcata gccttgccgc
       61 atccgcttgc cagtaccgac accgctgcaa taattgctgt taatgttatt ttctttttta
      121 cggacatata ttttcctttc ggtaattttt tacaatctgc attaaaatac gcctcgtaaa
      181 attccccgca aatacaggat atacatttga attatacaat atctccactg ttgaagtcaa
      241 ccgtatcggg ttaattttct caaatttgca cttacggata aaattttcta tatatccgat
      301 aataaaccgt atttgcgctt gtgcaatatg actaaacgaa atttgcaatt cttattcttc
      361 attctttcat aaagctgtgc agattcttga cttttacttt aatataatgt ataattaact
      421 gtaatctttt tttcaggagg acaaaaatgg gaaacaacga aagcaaggga cttaccctta
      481 ccgaaaaaat catcaaggat cacataattg acggtgagat gataaaaggc acagaaatag
      541 gtctgcgtat cgaccagact ctgactcagg acgctaccgg tacaatggca tatcttcagt
      601 ttgaagctat gggcatagac agagtaaaga cagagcgttc gctcgcttat atcgatcaca
      661 acacgctcca gtcaggcttt gaaaatgccg atgaccacag atacataggc agtgttgcaa
      721 agaagcacgg aatttatttt tcacgtcccg gcaacggtat ctgccaccag gttcaccttg
      781 aacgtttcgg caagccgggc aagaccctta tcggctctga cagccacact cccacaggcg
      841 gcggtatcgg tatgctcgca atgggtgcag gcggtcttga cgttgctgtc gcaatgggcg
      901 gcggtgcata ttacataaca atgcccaagg tagtaaaggt aaacctcaca ggaaaactga
      961 atgactgggt ttccgcaaag gacgtgatct taaaggtact tcagataatg tccgttaaag
     1021 gcggtgtggg caaggttatc gaatactgcg gcgaaggcgt aaagagcctc agcgtacccg
     1081 agcgtgcaac aattaccaat atgggcgcgg aactcggtgc tacaacatca atattcccct
     1141 ccgatgaaac aactcttgct ttcttgaagg cacaggatcg tgaagaagac ttcgttgctc
     1201 tcagcgcaga tcccgatgcc gtatacgacg aagagatcaa cattgatctt tccgagcttg
     1261 ttcctatggc agcttgtcct cacagccctg acaatgtaaa gactgttaag gagatcggtg
     1321 ctatcaagat agatcaggta tgtatcggtt catgtaccaa ctcctcctta caggatatgc
     1381 tgaaggttgc tcatatatta aagggcaaga cggtacatcc cgatgtcagc ctttcgattg
     1441 ctcccggctc aaagcaggtc ttcaatatgc ttgcagactg cggcgcatta tcgatactca
     1501 tcagtgccgg tgcaagaata cttgagagtg cctgcggtcc gtgcataggt atgggtcagt
     1561 cgcctaattc aaagggtatc tcactgcgta ccttcaacag aaacttctta ggcag
//
LOCUS       DAWAOG010000511         1611 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10022_length_1611_cov_1.663239, whole genome shotgun sequence.
ACCESSION   DAWAOG010000511 DAWAOG010000000
VERSION     DAWAOG010000511.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10022_length_1611_cov_1.663239"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          453 a          344 c          399 g          415 t
ORIGIN      
        1 gcccaagata gccaactacc atttcactac tataaccccc gttctcggtg ttgtaacagt
       61 cggcgaaaag tcatttgtaa tggctgatat ccccggtctt atcgagggtg caagcgaggg
      121 cataggcctt ggacattcgt tcttaagaca cgttgaaaga tgcagactta tagttcatgt
      181 agtcgatgtg tcgggcattg agggcagaga tcccaaggat gactttgaga agataaacta
      241 cgagcttaag aatttctcgg aagagatatc cgagcgtcct cagatcgttg ttatgaacaa
      301 gtgcgacctc gcaagtgaag agcagatcgc ggatttcaga aagtacattg aagataaggg
      361 gcttccctgc tttgagtgca gtgcggcaac cacaaagggt actgcgcagg ttatcgagta
      421 tatcgcaggc gagcttgaca agctgccgcc accgatacgc tttgaagctc aaccgcttac
      481 tgccaaagag cttgacgagc aggaactcaa aaagaaagat tttactgtca caaagcagga
      541 cggcatatat attgtcgatg caccgtttat ggttcctgtg ctttcgatgg tcaatatgga
      601 ggactacgag agtcttcagt atttccagag agttctgcgt ttcagcggaa ttatcgacaa
      661 gcttgaagaa atgggcattc aggaagatga caccgtttct atctatgact ttgaatttag
      721 gtatctgaga tgatgtatga aattatgacg actgccgaag cgaaagcgct cggagggctt
      781 acacttgcat tcttcggtga cagcgtatat gaggtattgg tgcgtgaaca gattgtaaag
      841 ctcggcacaa tgcctgttga taagctccat aaaaaagctg tacagtatgt aaatgcagga
      901 tttcagtcct atgcgtatga tgtgatagca gatgagctta ccgaagagga aacagagata
      961 tccaaacgcg gcagaaatgc cacaggaaat aatgtccccc gctcttcaaa tcctaaggat
     1021 taccgcagag caacagggat agaggcgctt ttcggatatc tgtaccttac agggcaaagc
     1081 gacagaatga aagagctttt tgaaaagatc tatacgagcg agcgtacata atttgcgaga
     1141 gaaggatcac aatgaaagca acaacaaaac gcacgctgat cgatattacg gtcattatta
     1201 tttcaagctt catattctcc tgcggtctta agacctttac cgctccgaac gatatagcac
     1261 cgggcggcgc atcgggaata tgcgttgcgc tggctcatat taccgggctt agcgaaggtg
     1321 tgctttactt tattgtaaac gttccgctta tagtgatagg atttatattt cttggcagaa
     1381 agctgatgtt caagacactg ctttcaattg tcactattac tattgcaacc gactatgtat
     1441 tcgcttatct tccccactac gagggaaacg aaatgctcgc agctatattc ggcggactgc
     1501 ttatcggcgc aggtttgggt ctttgctact cccgtgaagg cacaggcggc ggcactgata
     1561 tcctgaaccg gctcatttac aagcgctttc cgcatatcag cataggtgct a
//
LOCUS       DAWAOG010000512         1611 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10018_length_1611_cov_2.168380, whole genome shotgun sequence.
ACCESSION   DAWAOG010000512 DAWAOG010000000
VERSION     DAWAOG010000512.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10018_length_1611_cov_2.168380"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          333 a          488 c          302 g          488 t
ORIGIN      
        1 tcgctctcat acaccccacg gttgtaatcg cgatcatttt caagcacatc acgatcatac
       61 tttctgtcac tctcatacac cccacggttg taatcgcgat cgttttcaag cacatcacga
      121 ttatactttc tgtcgctctc atacacccca cggttgtaat cgcgatcatt ttcaagcaca
      181 tcacgattat actttctgtc gctttcaagt acgcttcggt tgtagtccct gtcattcaca
      241 tacttatctc tgtcgaattg agcctgccag tttctctgcg cctgtgcgtc cttattctta
      301 ttatattcaa actccctgtt tgtgttataa tcagccacgc tgtcacgata tctgccgtat
      361 tccgtatctt caagaccgat cagcgtgttg agctttttat acgcgctgtc ctcctcatca
      421 cgatagcgct catacgctct ctgttcaagc tccggtatct tatcattcag cttcgacata
      481 tagctgtcat acgcctgctg acccgcagtt accgcatagc tgtttgcata ccccccggta
      541 agcatagatg catttcctac cgtatcctcc atagccgtct tcccggcttt cgtatagcta
      601 tccttatact gtgaaaaaag cttgtcgctc ccgatatcat aattgaattt ctttccctcg
      661 agcacactcg aaagattgct cttgaccgca tccgaataac gactcttata ttcgggcaca
      721 gcggattttt tattactgtt ttttactgta ctcatatttc ctccgcataa cacatttcct
      781 gctctgcctt catctctgcc tcatactcat ctatctccgc gtcaaacctt gcgatatcct
      841 ccgccgttat cctgtcacgg ctgtaatgat taccgagctg catagccgcc catgctctgt
      901 caaagctgcc gcttttcacg ccgttcacag cgacctcaac gatccacttt ccgaaatcaa
      961 gcattatacc tcacctcccg atactgccgc cgcttcgagc gccgccactc ttgcttcaag
     1021 gcttgttatt ctgcgttccg cgtcctcagg agcgggactc cagtcagtcg gcttgttgcc
     1081 cgcttcaagc ttcatagcgc agacctctat atagtttccc gccgccgtag gctgcagata
     1141 tattctcgcg cccgaatatg tcccggtgct ttgcggtgaa aatttagccg taaagctgat
     1201 atactgccat tcgtccgaga taattatatc ctgtgtgttg ccgctgtggt cggtggtcga
     1261 tttatagaac ggctgtaacc tgcacggaac tcccgccgcc gaattactgc gtatccggca
     1321 ggaaaaagta tatatcactc ccgaattaag cggtatatcg tcctgcccac aaattatctg
     1381 actgttttca tccgtagaag tcagtcgtat ccctttgctt acacacgata tcggcgtgtc
     1441 ggtgatatca acggtctgta tcgttcccgc cgttccatat tttcgccagt gacccttgct
     1501 gtgaccgccg taaccgataa ccgcttcagc cgttcctgtg ataatattcc tgccgcctat
     1561 gctgactgca tttacagccg ccgtgatatc cgccgcagtc gccgcaccga c
//
LOCUS       DAWAOG010000513         1610 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10028_length_1610_cov_2.131833, whole genome shotgun sequence.
ACCESSION   DAWAOG010000513 DAWAOG010000000
VERSION     DAWAOG010000513.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10028_length_1610_cov_2.131833"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    637..646
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          407 a          364 c          350 g          479 t
ORIGIN      
        1 gcatgagtat atcggtaagg gtgttaacgc gccaaagtat cagagtttta cctcgtgaag
       61 aaagcttgct tgcctgcgcc gcaaagatct tgccgtcaat gcttactttg tccgtcagtg
      121 atgtatcatt tattcctttc gggaagatta tagccgttcc gcatgctgag tttctccagt
      181 gcacatttcc gtattcatcg gttaaaaaga aagggattcc cgaaccgccg taaagcgttt
      241 caagggcatc gaatatttta tccatggcaa tctgttcctt tacggcttat tttacttctt
      301 gtcatctttt atcaattttt ccggcttacc tttttcatca acaacatata ccctgtcaag
      361 ttcggacata agacctcttt taaaagaggc tctgtattcg tttcgtaatt cagtctgctc
      421 ctgcttttct tcgggagtga gctcgcgctg ccttgactga cgagcaagat aatttattct
      481 ttcgatttta gctttttcca taattattcc ttttatctga gatttttaat attatcgatt
      541 attctgttcg gctttttcag aataagccat actgccgcag atatgaatgt gccggtaagc
      601 actgcggcaa ttgccagaaa cggcagatat gtaaacnnnn nnnnnnttgt gcattacagc
      661 ggaaaatatc cctgcgggaa atacacccca cggttctttt ataaatcggc gggttatagc
      721 cattgccgcg aatgacagcg caccgcccgt gagtgaaaag agcaacgcca taaggtttcc
      781 tgatatcaga gctgacagaa gtacacgggt tatcaatatc aatgcggtat cgtatttgct
      841 ccaattcccg ccgagataca gcgagaaaag caccgggaaa tatgcaagtc ctatcctgaa
      901 gccgggtatc ggaacgacag gtggtatcat tccctctatt acatacaacg tcagcgacat
      961 tacggcaaga agtgccgcaa atacaagttt cttagctgtc atggctgtac tccgtgcggt
     1021 tagtttcaat gcggatgacc agcttatgtg gcatacatac gattggttga atgccgtccg
     1081 agatcggtgc tgtcataacg catatcctat cggggcagtc ggcatcggtt acgcttatac
     1141 tgccttcctt taccgctatg acattatatc cgctgtcttc agaatatacg gtaaatgtct
     1201 gatccgcggc attacgcagg tctatcgttt ttatgagttt cccgtcactg tagatacaag
     1261 cggtattgcc gccgccggta tttcttacaa gtattgcaca gagaagtgag aagaaaacaa
     1321 tgcataagac agcaataatg cagataacct tccgcttatc cgtcatgatg ccgtgaagtc
     1381 ggcggcaatg ccgtcagacg tgattattgt gccgtccgtc aaagttatga tatattccgc
     1441 aatgttatat ttactgtaaa attccgctac cttgtcagct cccatgacaa aaaaagctgt
     1501 ggaaagagcg tcgcttatca gtccgctttc cgagtaaact gtacagcttt ttacaccgct
     1561 gtctgcgggg tatgaggtgt cggggtctat tatatggtga tactttacac
//
LOCUS       DAWAOG010000514         1607 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10056_length_1607_cov_2.023196, whole genome shotgun sequence.
ACCESSION   DAWAOG010000514 DAWAOG010000000
VERSION     DAWAOG010000514.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10056_length_1607_cov_2.023196"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          432 a          381 c          350 g          444 t
ORIGIN      
        1 cttgacgagc tgattttact ggcggaaaag cacagcgttt tctttatgga agcaatgtgg
       61 atgaagttta taccagcatt cagaaaagcc aaggaatgga taagttcagg caagatcggt
      121 gatataaagc ttgtccgcgc cgacttatca tcattctgcc cgtatgaccc gaacgacaga
      181 ctttacgcga actctctcgg cggcggttct ctgctcgacc tcggcgttta tccgctgaca
      241 tttgcctgtg attttctcgg atataagccg aaggagatca tcacaagcgc atacatcggt
      301 aaaagcaatg tcgactttga cgcttccctg ctgctgagat atgataacgg aagctttgcc
      361 gactcgcaga taggatttga ctatatgaaa gaaaattcag cctgcataat cggcgacaaa
      421 ggcagaatta ctttcggcaa ctggttcttc tgcacgaccg atatcaagct gtatgatgaa
      481 ctcggaaacc ttaccgaagc gccgacaata caccataact gcaacggcta tgaatacgag
      541 gtgcaggagg ttgaacagtg ccttgacagc ggcaaaaggc agagcaatat aaatccgctt
      601 tggcagacaa gagccgttct tgaaataatg gacgaatgcc gcagacagtg gaatttgaaa
      661 tttgacggtg aataaaagca taccccttgc agtaaatttg caaggggtat ttgtcagttt
      721 acctttatct tgtccagcac ttctccgact ttatcatgta tcacaaggtc agccttgttg
      781 tcgtaaggcg tagacgacat atttataaga actaaattac tgccgttaaa ataacgtata
      841 aatccagccg cgggatatac ggtaagcgaa gttccgccga tgatcagcgt atcgcaccgc
      901 tctatagcgg aaagcgcacc tgttaccgtc ctgtcgtcaa gattttcacc gtacagcacg
      961 acatcgggct tgattattcc gccgcattcg caatgaggta ttccctcgct gtggagcata
     1021 tattccgcac tgtagctttt tccgcactta aggcagtaat ttctgtgaac acttccgtga
     1081 agctcatata cagtcttact tcccgccgca gtatgcagtc cgtctatatt ttgcgttatc
     1141 accgctgtaa gctttccggc attttcaagc tcagagagct ttaaatgcgc cttattctgc
     1201 tttgcgtcaa ggcagagcat tttctcgcgg taaaattcaa agaatttttc ggtatgagaa
     1261 aagaaataat cgtggcttaa aagctcttcc ggcggaacat catatttttg attgtacagt
     1321 ccgtcaggcg aacggaaatc aggtatgccg ctctcggtcg atactcccgc accgccgaaa
     1381 aaaacgatag acttgctctc atcgactacc tgttgcaatg ttttcatata aagaccctct
     1441 ttcagatatc atcgccgttt tcatggcaga aatgacactc gccgcaaagc tccttgtgtc
     1501 cgtatacctc ttctattata ctacggttgt caagtagctt ttttgcaacg tcagggtcgt
     1561 acatcagtcc gatattcttt tctatttctt catacactgt atcaagc
//
LOCUS       DAWAOG010000515         1603 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10094_length_1603_cov_1.879199, whole genome shotgun sequence.
ACCESSION   DAWAOG010000515 DAWAOG010000000
VERSION     DAWAOG010000515.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10094_length_1603_cov_1.879199"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          386 a          371 c          304 g          542 t
ORIGIN      
        1 caagcaataa gcgacaccct atttcaatgt ttcaaaaggt tttttgacag actgagccgc
       61 ggactttcgc ccgcggctta ctcttatact gttattgtta tgctccgatg ctgtatcagc
      121 tgttatctgg gctgcttttc tcttcactgc tgctgtcaga gctttcgtcg gcggcataat
      181 gcatatctgt catcaaacgt ttgaaatcgg ctgaatcctt tgccgttcct acaagccatc
      241 tgtgcttgtg tcccggttca gtcggagcta ttatgtcatt accatagcag ccttcataag
      301 tatatattgt agccatgtcg ccgctatcct caatgaatat attgataata actcgttcag
      361 gtttgcttac ccaataactc agacacaaat attgaccgcc tcttccgtta tcagttcttg
      421 aaacgtccat taaagggtat gttttgtctc cgatagttat tgtaccgttc acacattcat
      481 taccgtttgc cctcttttca actgtagctt tgatcgctac ttccacgtgt tcaaggttgt
      541 catactgctc ttcaagattt acatgatcac cgtatatatc agcaaacgta ccggaaatat
      601 ctacttcctg cttaagcatt gatgtatagc ggataatatt ctgctccttg ataactctgt
      661 cgaagctgtc atcatctgtg gcaggacctg cccatgtgcc ttccttttcc gggttttcca
      721 acggtataac gcttacaacc gcgctgtttg tacctgttgc agttctgaca ataaggtcaa
      781 gtataccttc ttcgcttctt gaattaagcg tcattcccga ttgatcggag cttgcagaag
      841 tgatcttata ctcagtgtca cctaccttta cagtgccctc cgcacccgtg acagtaacta
      901 tatttttggt aataaccgca tctatgctta tcacagtatg ttcgagtaca tcaaagctta
      961 ctgtgtcact tagagttctg ctgatatctg caaaagtacc gctgatttta agcgcctgcg
     1021 tggtagtgtt cagcattagc gcgataacga ccgtaagtac tacaataacg aatagacaaa
     1081 tagtgataat tattttctga aattttggca taaaatatcc tttcccgtat gtttttactg
     1141 catttgtttt gctttcgcca atgtgtttgc tttgctattc gatgtataaa gcatcgaaat
     1201 gttattagct tttttgttgc gtaaatgtaa ctgcgctttt gctaaaattt tatatgtatg
     1261 tgatatcagc ctccaatcta tatttatatt ttaccatatt caaatatccc ttgtcaacaa
     1321 acaaatcaac ttttatgtac gcaaaagcgg cacagcataa tgccgtaccg ctccgccgac
     1381 tatttgctta taacaactta accgagtgtg cctgtaaccg taactgtcgt tttaccggga
     1441 gttactgcta ttgtgccgtc ggtattggtc gtgaatgttg ccgcaggcac tgttatagcg
     1501 ccctgagccg tttcacacac gcctgttgtt tcatcatagg tgtacttggt atcatcaagt
     1561 gccgcgccgt tatagcttac cgtaatgcct ttgagaatgg ggt
//
LOCUS       DAWAOG010000516         1590 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10198_length_1590_cov_1.561564, whole genome shotgun sequence.
ACCESSION   DAWAOG010000516 DAWAOG010000000
VERSION     DAWAOG010000516.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10198_length_1590_cov_1.561564"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    605..614
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          465 a          371 c          283 g          461 t
ORIGIN      
        1 ctgctatttc ttctcaccga tccggtgaaa ccgccgatac aactatcgca gacctcgcgg
       61 ttgcacttaa cacctgccag ataaagaccg gtgctcccag cagaagcgaa cgtgtggcga
      121 agtacaacca actcttaaga attgaggaag agctcggtga cagcgctgtt tatcccggta
      181 taaacgcatt caacgtaaag cgttgaatta aataacgcat tcgtctcctt tcaaataaca
      241 aaaactcccg ttagctttat actgcaacgg gagtttttgt gttatagaaa tattcatgaa
      301 aaacttaaag cggcgataga agcttttcac cgccgcttac cgaattttat tccgaataac
      361 atttcatgac tttcttcatc tcttccacac ccttaaattg agactctatc atttcattca
      421 gcatgagtct cagccgagga catatgttga acctgagtgc attcctgctc attgctatgc
      481 cgccctcatg atgcggtatc atctgcctta tatagtttat attgatgttg ttgtcgggac
      541 acgccgactt cattcgactg aacatattct gcgttacggt accgtactgt cttctgtaag
      601 cacannnnnn nnnntcttta gtctgaccgt ttattatttt ctgtgctgtc tcctgaagac
      661 atatatcggt agtatattta agcagttctt cagaaaattt aacagcagcc tcgtggtgtg
      721 gtatcatagt tacaataaaa tcagccgata cactgtcgtt tatccttata ttactcatat
      781 ttgagatcat gttgttaagt atcttttcac tgcatacaag atacttctgc gtgactatgc
      841 tgaatgtaca tttttccata attacctccg atatgtttta taccagtcta tgaaaaacgc
      901 acggatttgt tgacatcata tgcatcacct ttcagatgtg ttgcaataaa gacggttaaa
      961 tgaaatttct ttcacaaaag gcatttaaca ggctctgtat ttgacttttt acctgtgatg
     1021 tgctaaaata tcatttgcaa agccattttg actcacttta cccctgaaag gatttaacaa
     1081 aaaatgaaat cattatatat aaaactgata gccgcttctc ttgcgctttc ctgcactgcg
     1141 gcacttacag cctgcagctc ttcacagact tcttccgata catcatcaga tgccgatatc
     1201 ggaggttcta cctctgtatc cgagcaggaa aacaacaata ataccgaaaa ttccggtagc
     1261 tcagaaaatg tagactcctc tactcactcg aacggcaact ctgccgataa ttccgacagc
     1321 agtacaaccg aagataaacc ttcagcctcc agcaacaccg aaagcacagt atcgtcagta
     1381 acatacgaac ccgtaaatga cggaaaattc acgcttgcaa atgaaaagct gtctttcaaa
     1441 atgcctgacg gctggaagct tgtcagtgca tctcttgcgt atcagtttgc atctgaggac
     1501 gaaacagacg aaaacaaatt caacctcgtt gtcagcaaaa ctgacagccc gatagaaaat
     1561 gtgacatcag atcagatgac agccacctat
//
LOCUS       DAWAOG010000517         1586 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10226_length_1586_cov_2.100588, whole genome shotgun sequence.
ACCESSION   DAWAOG010000517 DAWAOG010000000
VERSION     DAWAOG010000517.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10226_length_1586_cov_2.100588"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          429 a          377 c          287 g          493 t
ORIGIN      
        1 ttaatatgaa acaaacccga caggtacgat tcaataaacg tatctgtcgg gtttattttt
       61 caactgagta tatcgcatct ttcgtttatc agacgtataa agcattcttt cagattatcc
      121 gacagcaacg aatcacggca catagcttca tatttttctt ttaaagatac tacccttttt
      181 atcatattct cagcagaatt tttaggaata ccgcatttat atgcaaacgc atagaaatct
      241 tttttgcgta tatttctctt cttaccgttc agcgtaagag cggtctgctc aatatcggca
      301 ggaagtatga catttaccgg taacaagtca tacgcaggtg ataaaacata ttcaccgctt
      361 ttttcggcag tttctatcag cgagaagttt ttaagatgca tatccgagtt tcctgtcaca
      421 aaagaaaaaa caagccgcat aaaaagctcg gtcatatcaa atcccaccct tgacgagtat
      481 cgttctatca cctttgcaca gcgctcatac gagcctttat atttatcctg cgtaagcctt
      541 aaatcaagct ggcagaaatc ctccattgca agcattgcta ctctgtcggt gtacattatc
      601 ctgtctgctc tttttgtaat atacgcataa ttaccgttat ttcctataag agcgtgcgga
      661 acagttgata tacctgttat gtccgccata gacattacaa gatgctcggc ttcgggcaga
      721 ttttcatatt gagtcacctg aggcttcaga atataaccgg tcgggaaatt tacaagcgtc
      781 agtttaggcg actttttttc cgaaataagg tgcaatgaca gctttttctg cacaccggga
      841 acagtaaagc cgctgtttac cgtttcatct gcaagctttt taagcgttac atcgtctatc
      901 tctatttcag gtagtccggc tacgccgaaa aatcgcttta tacagctttt atgccagccg
      961 ttttcgccgt ttttgtcaat atcctttccg caacacagac atttcataat ttctcctccg
     1021 taattgtaac attgcctata cagtccttgc aggcaaccaa taacagtcca aatctgtcct
     1081 tattgtcgag cttccagtta cggctcacca catctaaaag ccacccctcg ggaatcagac
     1141 cgtcaaaaaa agaaaacagt gtttttgagc tgtaaacttc atcgctaagc ggcaatgtaa
     1201 gtgaaacagc acttgcgttt ttaccggcaa ggtaagagct gtcataggta aaggtataac
     1261 cctcatcggt ttcgcaaagc tgtcctgcaa aaacgtttct gacatacaca taagctgttc
     1321 tgagtatcgt cactgtctgc cgtcctttct accgggcact gcttccatat caaacatggc
     1381 aagcgctgtg ttgaccttat ccatacgcac ggtttctttt ccctgttcaa gctcacggac
     1441 aaagcgcaga ccgagcccag aacgcattgc aaattcttcc tgggtgagtc ccgcttcttt
     1501 tcgctttgtt tttatgaact gagctatttt gttcaagtat atcatctccg ctgttgtaat
     1561 acgaatacat tttacaccct ttaggg
//
LOCUS       DAWAOG010000518         1583 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10248_length_1583_cov_1.992801, whole genome shotgun sequence.
ACCESSION   DAWAOG010000518 DAWAOG010000000
VERSION     DAWAOG010000518.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10248_length_1583_cov_1.992801"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          355 a          379 c          381 g          468 t
ORIGIN      
        1 gaatatatga cgttttgtat agcggattta cgctgaatga tgcttcttat tttcctttat
       61 aataaaaacc ggtgcatgat gtaaagattg caccggtttt ttatattaca taattatatc
      121 ggctcttgtt ttcccaaaca tttattatag agccttcttt tattccacac aaaaccctcc
      181 cgtttgaagg gagggtcggt gtttattcat tacgttttgt atccgttaag atcaaactaa
      241 atcgtcctca ttgaagggat cgccgcattc ggggcagaac ttcggaggat gctcgggatc
      301 tgccggttcc caaccgcact tgtcgcactt atactttttg ggagctgcag gctttgactt
      361 accgcattcg gcacagaact tgcctgtgtt tgtagcaccg cattcgcacg tccagcttga
      421 aggagcggga gttgcgggag catcaccttc cttgctcttg ccgcagttag cacagaactt
      481 gcctgtgttg gactgaccgc atgagcaggt ccatgcctca ggcttcttag cgccgcagga
      541 agaacagaaa gcacctgtgt ttgtagcgcc gcatgagcac ttccaaccgc ccggtgtggg
      601 ttgctgaggt gttgtctgct gaggagccgc aggcataccg ccaagcacgt tgttacctgc
      661 gttcatagcc atgttcattc ccataaagcc catcatagcg ccgttttcgt ttgaacctgc
      721 ggcttcaata ccgcgggcaa ccgcacctgt catcatagca cgctgaacat cgccgccgag
      781 cattgtatca gccttaagac gatccttaag aagttctttt gtttcctcgg agatggtgat
      841 aggaccaaga ccgatatttg tgagcatcag acctctctgt gcccagttat tagctgtttc
      901 gtccttagtc ttgttggtaa gattgttgag ctggcttgta atctggttgt agctgtaacc
      961 ctcagctgaa agctggttta atgctaccat cagcgactgc atgaactcgt tcttgtactg
     1021 ctcgttcttg aaaatgtcct taacgtagac ctgaggagca ttcacgccct tgctgagaac
     1081 ttctgcatag aacttgcagg cattctcggc atcgggtatc tgaacctcga aagtgccgta
     1141 gaaacgcaaa tctactgtcg tattataacg gggatcggga tacggaacag gtgttcccgt
     1201 accgaatggg ataccgggca gtttctgaag attgatgtaa attactctct gctcggttgc
     1261 aggtgttccg ccgtatgtaa atcttgaaat aagatccttg agcgaatcct tgatctgacc
     1321 tgcaaagatc gaaggtgcgg tcgagttgtc aagtatgtaa cgtccgggct ctgtaacaac
     1381 gttagttatc tttccgttgt caagtgtcag catacaggtg ttttcttcga ccataatagc
     1441 cgagccgttg ctgataacgt tatccgttcc cttagtgtta ctgcttctcg cattaccgtt
     1501 gtgcatcttt tcaccgttga caacaactgt atcgttgccg ataacggggc agtgaatggc
     1561 ttctttccag gtatctgcga gcg
//
LOCUS       DAWAOG010000519         1582 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10258_length_1582_cov_1.865750, whole genome shotgun sequence.
ACCESSION   DAWAOG010000519 DAWAOG010000000
VERSION     DAWAOG010000519.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10258_length_1582_cov_1.865750"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          441 a          368 c          284 g          489 t
ORIGIN      
        1 ccgccgaaag ctctatgaaa cgctctcctg ccgcaactgt cacctttccc tctccgctct
       61 cgtttgcggg aatagggata tacaccctgc ccttttcacc gagcagtctg cgcacatcaa
      121 ggcttccgtc ctgcgtcagc ttctttgtta gctgaagcac ctttgcaaca ccgagcattg
      181 caaggaatcc cagcacaagc gcaattacta tagctatcgc aacgtgaagt ccgagcgagg
      241 tgcatataac acccgtccag ccgaatacgc acaggaatgt cataattccc tgcaatgtga
      301 aaagctgcat tgttccgaag tctgacggat tagaaccgtc gccgatagct gtatggtcac
      361 aaccgtcaat tgcatgagca cccgtaatat caggtgcatc tgtcggaata tcggaaacat
      421 ccgaaatgtc aagtccgtca atcccgcttg tgtcactaaa ctcgacccct gtcccgccgt
      481 gacctatacc tatcatcagc aatatcgtct gaatgacgat tataagtgtt gcgggaattg
      541 ctatacaata aaagatctgc attgtaatcc ccagcgaagc ccaccattct aacatatctt
      601 tcaccctttc aaatttcata tcaaactgct tttgtgaaca gtccaaacat aatagcaaca
      661 agtaatttta cagcctgtta aatttagttt ctctattacg aacagtaaac tttgcgtttg
      721 ttcatttcgc attattatgc ggtttctcta tctcgtataa gtatctcttt ttgcgctgta
      781 ttatgctaaa ttcactttgt tgaaataacg atgcgtatat tctgtttaac aattactcta
      841 tgtatatcaa ttgttgtagt atccgagcgt aagcaagata aggaaattct tatctgcaag
      901 cacccactca tcattgatat aacggaaatc tacagtaaac tcaaacttat ccgatttgaa
      961 ctcgtcatca ccgataagcg aatcgatgta atccatctgg gcgttgaaat cgttctttag
     1021 ctcactgttg atcttggaat cggtcttttc gttgaacttc tccatatcaa aagaagtgaa
     1081 cgtaccggtc acgctaccgg ttctggcagt tttatcggtc ttttcggtaa gattatcaca
     1141 agttacataa tttttgaata acgatgccat agtttcagaa taacgtgata gccagctatc
     1201 ttcatatgca gtatctgtat ctgaaaagta aagctccatg cttctcttgg aaaagctgcg
     1261 gcaatatgaa gcaagatcgt catactcacc tgctttggca agactcatta tctcttctat
     1321 ggttccctta ggagttaagt gcttggtttc ggttgccgtt tcacctctgt gacatcccgc
     1381 aaaagtgagc ataaatacca tggtacatac caaaattaag gctcttacgg cgtatcgttt
     1441 tatttttata tatgaaatat tcaactttat tagtcctttc tgcttatagg cagtagcaga
     1501 ttttgctatg taaatttaat ttaccttaat tatagcacat ttgaacgaaa tgtcaatatc
     1561 aaaccttgcg tttgctcaaa at
//
LOCUS       DAWAOG010000520         1580 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10270_length_1580_cov_2.550164, whole genome shotgun sequence.
ACCESSION   DAWAOG010000520 DAWAOG010000000
VERSION     DAWAOG010000520.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10270_length_1580_cov_2.550164"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          444 a          330 c          450 g          356 t
ORIGIN      
        1 aatgcgtcag atacttgaag atgtcgtaac cgcgaacggc ggcggtaatg cttacatgag
       61 cggctatcgt atcggcggca agagcggtac atcggagaag atagatgact acaacagcgg
      121 aaaaacccct gagctgagat atgtagctac attctgtgcg atagttccga tagacgatcc
      181 cgaatatgta atgcttgtcg tatgcgatga gcctacatcc ggttatattt acggcagtgc
      241 gatcgccgca cctgttgtat ctgcggtatt caaggaagga cttgaatata tgggtatcta
      301 tccgcagtac acagctgatg agctggctca gcaggacgta accgttccgt gggtaggcgg
      361 atataacagt atccgtgcag aggcacagct tacagccgca ggacttaagg ctgagtatat
      421 aggcagtacg gacggtacag aagtaacagg tcaggttccg tcagccggaa cggtaatgcc
      481 gagcggcagt acggtaatgc tctatatggg agatataccg ctgtcggatt acagaatgtc
      541 aactgtaccg aatgttatag gaatgacggt agagaaagct aataaagcgc tcagcaatgc
      601 cggactcaat atcagcatca gcggagcggc tacaggcagt gaagcaaagg ctgtaagcca
      661 gagtgtgaac tccggacttt cggtatacag aggtactgtg atagaggtca acttccttgt
      721 caataatgaa acaggctgat aggagagggt attatcaaac tgtatgatat ctgcccggcg
      781 gctgaagccg cgggtataga aaacacagag gtgacaggcg ttaccgatga tacgcgtgag
      841 gtcaccgaag gcagtatatt tgtctgcata aaaggcgcaa attttgacgg tcacagtgcg
      901 gcgcggaaaa tgctcgacag cggcgctcaa ttggtagttg ccgaatatga tacaggctgt
      961 gagcgtcagg tcatagtgaa aaacacaagg cttgagcttg caaatctgct ttcgcacttt
     1021 tacggcgagc caacaaagaa gtttaaactg cttgctgtca ccggaactaa cggcaagacc
     1081 acaacggcgc actttgtaaa gcatatactt actgttctcg gtaagaagtg cgggtgtata
     1141 ggcacggccg gaaatgatac ctgctccggt gagctcaaac cctcacagca tgggactccg
     1201 acagtgccta gagcaacggt gctgtataag tggtttaaag aaatggctga taacggcgcg
     1261 gattactgta ttatggaggc aagctcaaag cgccgtgtgt ttacaatgtg caaaactgcg
     1321 gtagtaaata tcgatgatga atatggcaga cggcttgccg atgaattttc cgacattgct
     1381 gtgacctaca gcgtaagcgg caaggcggat atatatgccg ggttcataaa aatgaacgcg
     1441 cacggttcgt cgtttatgat agtcaatgag cgtgagcaca gggcttgtcg ggtagatata
     1501 cctatggcgg ggctttataa tgtgcagaac gcactgtgcg caatagctat ggtgacggcg
     1561 ctcggatacg atatgaccga
//
LOCUS       DAWAOG010000521         1580 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10272_length_1580_cov_2.166557, whole genome shotgun sequence.
ACCESSION   DAWAOG010000521 DAWAOG010000000
VERSION     DAWAOG010000521.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10272_length_1580_cov_2.166557"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1170..1179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          388 a          408 c          303 g          471 t
ORIGIN      
        1 caatacttac tgcttcatct acactgccaa caagcgttag catagcatca acagtatccg
       61 ttgtaagctc cgtgccatta gcaacagctc cgactgtatg ctcgataagc tgtgatgtaa
      121 gcgccgccgc atctgcggtt ttgcccgcaa ggtttcttac ttcatcggca acgaccgcaa
      181 agcccttgcc tgcctcaccg gctcttgccg cctcgacagc agcattgagt gcgagtatat
      241 ttgtctggaa tgcaatatcc tcgaccgtct gaataatctt tttgatctct cccgacttgt
      301 cgccgatctc ctgcatagcc gctgaaagct tgttcatact gtcattacag agatttacct
      361 tttcgcccat ctcagtcact atcacatttg cgcgatttgc attgtcggct gtggtcttta
      421 cgtttccgga tacaagctca cagttcgacg aaagcgtatc gatagccccc gcctgcatag
      481 ccgcaccgtc agaaagctcc tgtgcgcctg ccgcaagctg cgcagaaccc tctgatacct
      541 gacctgccgc tgtttcaatc tgtgtgagcg tatctttaag tgcactctgt atctttatca
      601 ttgaagcttt tatatcggca aaatccccat tatactgcaa tgttatctcg ttattgaagc
      661 tgccatcagc cataagacct agctttccgt tggcttcttc aatagctaag caggcagagc
      721 cgatctcatc gtttttgcga tatccgctct tatatgacag ctgaccgttc ttcatcgaat
      781 cagccgcctt attgatttcg ctcattggtt taagcttacg gtttatctga atgttaagta
      841 caatcgtgct tatcacagta agtatgacaa gtactgagat gaatattata accagccaca
      901 tctcgctaaa atagaaagta tatgtgtcct gtgcgtaaat tattgtccat ccatagccgc
      961 cgacgttttc ctttgcatag attgaaagct gaccattttc atctgtgcct gtatatatcc
     1021 ttccgttctc atttccggcg atcgccgcaa tacttgtatc ggaagccttt gtataatgtt
     1081 cgctttcatc attatctaca taaggcagat atgcatcatt cctatgtaca actatatctc
     1141 ctgcggcatc gataagtatc gggtaatatn nnnnnnnnng gactttgcta tatcaatgac
     1201 agacgttaca aagatatcgg cagcaactac cgaaatatcg ccgttatcgt ggaatatcgg
     1261 ctttgaaacc gttattacaa gctcttttgt cttgacatcg atatacggat cggaaacaac
     1321 cgtcttttta gcttcaactg cctttgtgta ccagtcacgg tcggtagggt cgtaattttc
     1381 tgcggcagca tcccaaccgt cagagaatac agatgattta tccgactttc cgatgtagaa
     1441 cacatatacc tcgtctgaaa ccgactgcat atcgacaaga aaaccataag ccttgtcgaa
     1501 attatcggca tcgtatttac cgctgtcaac agcctctgct aagttatcca tcataaagca
     1561 ctgcttatcg aaccatgccg
//
LOCUS       DAWAOG010000522         1580 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10275_length_1580_cov_1.961311, whole genome shotgun sequence.
ACCESSION   DAWAOG010000522 DAWAOG010000000
VERSION     DAWAOG010000522.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10275_length_1580_cov_1.961311"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          415 a          349 c          288 g          528 t
ORIGIN      
        1 ctataaagca acgagtattt cgggagcaca tcttctcctg caaggtatgt gcatttacag
       61 gtcattcttc tgataaatcc atcttcggat ttatcgtaat cgaccatatt ttcaaagttc
      121 cacggatata ttcttccttc agccttgcgt actatccaca cattgtcggt ttcatttctt
      181 tttaccgacg gtcccacaaa ataaggtatc ctgaatttca taagcgtaag aatcttttgc
      241 tttacagaaa tgccgtcctc tgtctcattg aggaaatcaa gataagccga tgctttatcg
      301 agtatcatac tcagctcgtg ataatataac tggtacggga taacacggtt ttcatcggta
      361 acctgcttgg ggcaaagtgt tttgctgttg cacttttcga tcagttcatt atataccgcc
      421 tcatcatcgg gctcgggttt tatcttttca agcttgcttt tgatatattt gcaaaattct
      481 tcagaatttt tctttttaaa gtttgacgga agctgcttta acttaacgtc ggttacgtta
      541 tatgagtagc tgacatagtt cggcttttca ccgactgtgc ggaatatttc atcatactga
      601 gccttatcaa gatatttacg gacaatgtgc ttaagtgctt taagatccga cttatgctgc
      661 tcgtattcgc acaccttggc atcggaaatt gttttttgac ctttcaatac atcaataagc
      721 aacgaccagt catacattgc ttttacggca gaaagcagag gaacatcggt atcttccata
      781 gaagaagcca ataaatcaat ggtgtctgca aaatccgctt ttccgacctc tacagtctgt
      841 atatcgagtt catcgtaagc cggatttctg aacagcttgg cgacatttgt ttttccgccc
      901 gatataaact taatcagaac gtcaatatct atcggatatt cttcgtcttt atagcagtcc
      961 ggtgtctttg ttgttccaaa aagcagattt ttgatttcct tctcttttgc cgtaatacct
     1021 atcttcttgc ggaggatgac agaaaactca ttctctgtgc tttccgaaaa aggattatcc
     1081 tctatatcgt tcgatttaaa ccaatccgta aattcgcaat aagaagaatt gaaatcaagc
     1141 acttcctgta cattattttc gtcagcgccg ttaaggaaat gtcctctgtg cgccactata
     1201 aaagcacagg caagataaac gagcctcaca tcgtgcggct tatcgctttc agaaagctct
     1261 tttataaggt gatgaacggt cggatacatt ttaaaatagt cactgtcttt aaaatccgcg
     1321 tcatcaaagt atgtattgac atttttgttc gttctgtctt ccggataaag agaactctcc
     1381 tttaacctca aaaagaaatc cggatcggtt ttcagaattt cttttgcaaa cagctccctg
     1441 agcagtgcca ctctttgttg tctgcgatcc agcctgcgtc ttgcggttct gtgtcccctg
     1501 cgttcttctg ccggagaagc agcttcaaac agacttactc cccacattaa attatttttg
     1561 tatttgtaca gcttgtacaa
//
LOCUS       DAWAOG010000523         1579 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10283_length_1579_cov_2.392388, whole genome shotgun sequence.
ACCESSION   DAWAOG010000523 DAWAOG010000000
VERSION     DAWAOG010000523.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10283_length_1579_cov_2.392388"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    458..467
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1187..1196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          429 a          321 c          312 g          497 t
ORIGIN      
        1 agcccatacc gtgtgaaata aaaagtcatt cgcctgattt ctaaattctt tttccgataa
       61 gggaatataa cttacttttt caatcacagt aatcatatga agatattccg caatgccgca
      121 tctgtcaaat aacagtttgt atccgcggtt cataatttcg ttgatcgagc gagaattaag
      181 atgacttttc agtctgtctt cattaagtat tatcatatcg acatcaagtg agccttcaaa
      241 gagaatacgg cgttcctttt cacctgctat tgtatcctca acaaatgaat aaacaggcat
      301 accgagcttt cttatcaatg tatcctcata cagcaacttt tcaggatgtg agcatgcaat
      361 aatgaggtca acatctgaat actcatcagc tttgcaataa tctcttgcct gagagccgat
      421 gaggattact gcttttattt cctgttctgt ttcacatnnn nnnnnnnccc ttaaagcaaa
      481 agccacagtc agactgcggc tttttaagtt taattgttaa tatttaagcg gttcgagctt
      541 agtaagatca aacggtgttt cctgatatac acagagattg agccagtttg agtacataag
      601 gcttgcgtga ccgcgccatg taaaatgcgg tgtcagctta gggttatcat cgggaaaata
      661 gttgtacgga acttctatag gtatgccctt ttccacatca cgcatatact catctttaag
      721 cgtatttctg tcatattctg cgtgacctgt tataaagatc tgcctgtttg tgcgatcggc
      781 aacgatatac gcacctgcaa tatccgaata agcaagcaat gcaagatttg ggttgctttc
      841 tatatcttcc atcttaacgc ccgtataccg tgaatggggt accatgaaaa tctcatcaaa
      901 gcctttaagc agaggatgat ttatcgcatt tatcctgtga gggaagatac cgaacatctt
      961 tttatcaagc acctgcttct ggataccgta atgatagtaa agtcctgcaa aagccgccca
     1021 gcatatatgg acggtgctga acgcgtgtgt cttgctccat tccattatgt cacacagttc
     1081 tttccagtaa tgtaccgaat tgaagtcaag caattctaca ggcgcaccgg taataattaa
     1141 ggcatcaaaa tactgttcct tgatcttatt gaatgtggtg tagaagnnnn nnnnnngtgg
     1201 ttttcgggtt gtgggtttcc gttttcacga gggttatatc tacctgcaag ggagtgttgc
     1261 tgagtagcct taaaagctgt gtttcggtca cgatctttgt aggcataaga tttactatag
     1321 cgaccttaag cggtctgata tcctgtcttg atgctttttc atcagccata acgaaaatat
     1381 tttcgtttat gagcgtgctg aaagccggca gattgttagg tatttttatc ggcatttaac
     1441 atcatccttt tcatttcgtc atattactag attataacac gattttaatg tgccgtcaag
     1501 gcacatagcg gcaaatgccg caagttttgc ctatcgggca aaacaaaatc gtgtttcaac
     1561 gttctcagcc attgcgtag
//
LOCUS       DAWAOG010000524         1578 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10291_length_1578_cov_2.563362, whole genome shotgun sequence.
ACCESSION   DAWAOG010000524 DAWAOG010000000
VERSION     DAWAOG010000524.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10291_length_1578_cov_2.563362"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    731..740
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1239..1248
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          486 a          326 c          431 g          315 t
ORIGIN      
        1 atgataacgg gtataaaaag atcgataact tcaaaatcta ctgctacagc ggtacagccg
       61 gcgagcggta tgcaaagaat tacggttttg agtatgaact tttagatcat actgagcata
      121 catacggtga gtggaagacc acaaaggcgg caacctgcac ggcagagggt acgcagacaa
      181 ggacctgcga agtttgcggc aagacagaaa cacagacgat aaaggcgctc ggtcatacag
      241 aagtaataga cgaagcagta gaagcaacct gcacaaagac aggtcttaca gagggtaagc
      301 attgctcagt ctgcaacgcg gttatcaagg cacaggaaac agtacccgca acaggcaagc
      361 actcattcgg cgactggaag atcatcaagg cggcaacttg cacagcagag ggtacccaga
      421 caagaacctg cgaggtgtgc ggcaaggtcg aaacacagac gataaaggca ctcggtcata
      481 aggaagttgt tgacaaggca gtagccgcaa cctgcactaa gactggactt acagagggta
      541 agcactgctc ggcctgcaac gcggttatca aggcacagga aacagtgcct gcaaagggtc
      601 ataagtatgt tgacactgtt gtaaagccca catacactgc acagggctac acacttcaca
      661 agtgttcggt ttgcggcaac agttacaagg acacttacac agctaagctc gcgcttgcca
      721 aggtgaccgg nnnnnnnnnn caaatccgtc tgttataaag ggtgcaaagc ttagcggcag
      781 agcggctgac gcactgcgta tcaactggac taagaatgct tcggctgacg gatatatcgt
      841 tgagatgtat cagggcggta agtgggtaag agttgcaaag ataacaagca acagtacaac
      901 cacattcaga aaagcgggac ttagcgcttc aagcgtttat aagttcagag ttagagcgta
      961 caagatgagc ggtagcacgg cgctttatgg taattacagt gcgaccgtta cggcaaggac
     1021 aaatccttcg attgttaagg gcgttaagat cggcggtaag gctaaggacg ctttaagagt
     1081 gaactgggct aagaatgctt ctgcacaggg ttacatcgtt gagatgtaca agggcggaaa
     1141 gtgggttcgc gttgccaaga taacaaataa tagcactgct acattcagaa aggcaggact
     1201 tgcaaagaac acaacgtaca agttcagggt taaggcttnn nnnnnnnngg gtcggccgat
     1261 tgcgcccgcc gcacttgcaa aaacggggga tttgtggtac aataaaacta attattgact
     1321 aaagttgcgc tgtgcggggg agtacggcgg ttgaaaggat aaagctgatg gcaaagaaaa
     1381 attatgacga taacagtctt aaacagctga aaggacctga ccaggtgcga ctgcgcccgg
     1441 gtgttatatt cggctcggac gggcttgacg gctgttgtca ctccgttttt gaaattctct
     1501 caaactcgat agacgaggca agagagggct ttggtaacaa aataatagtt acacgctacc
     1561 ttgataaaag cattgaag
//
LOCUS       DAWAOG010000525         1578 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10292_length_1578_cov_2.293500, whole genome shotgun sequence.
ACCESSION   DAWAOG010000525 DAWAOG010000000
VERSION     DAWAOG010000525.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10292_length_1578_cov_2.293500"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          446 a          356 c          317 g          459 t
ORIGIN      
        1 ctgcctcctg tgtaataacg gatataagcg atacaggatt taccgctacc gtaccatatc
       61 ttgatctgta cgaagcacac cgcagcgcaa agctggaatt tgtacttgac agcggcacat
      121 ataagatcaa ccgttgggaa atgaaccttg aagcgatgaa cggcaaataa gcctttaaag
      181 ctattcgaaa aatatattat acaaaaaggc tgtgacgaaa tgtaaaaatt tcatcacagc
      241 ctttcgattt attaaaactt caaggtggtg ttaataattt cgtgagtaaa tccgtacaac
      301 tgcgctaaca tcagcccttc aaaaaaccgc cgtgaaaatc cacagcggtt taaaaatatc
      361 acaattacct acttctcata aaccgggctt tcaagtcttg ccgcaaactc ggtttcggga
      421 agcggtttat caaaataata gccctgaatt atgttacaac ccatattttt aaggttttcg
      481 acaatcgttt tatcttctac tccctcgcaa acagtagtca tactgagctc tttcgccata
      541 tgaagcacgt tttcaagtac gacctgacct cttgtatcgt catttgaacc tgcaagcgac
      601 ttatcaagct tcagaatatc aaaatcaagg tctttcagca gcgcaagcga agaataacct
      661 gttccgaagt cgtccataga aaccataagt ccatagcttt taaggtcgat tatcgcctgc
      721 ttgatagcat tgaaatcatc ggtataagcc gtttcggtaa actcgacttc aaggaagtgc
      781 ggttcaattc cgtaacggtt gataacattc attatcttct cggcgagatg agggaaacgc
      841 agatggatct ttgagaagtt caccgataca ggaactatgc gctttccgct ttcctgccat
      901 ttcttgatca tagcgcaggt gtgctccagc acgaacatat caagcttcat tataagacct
      961 gttcgttctg ctatcgggat aaagtcggcg ggagaaatga ccttgccgtc acgttgccat
     1021 ctgataagcg cttccgctcc tacaagagtg cgtgtgtcta tccgtacctt tggctgataa
     1081 tacacgataa attcgccgtc ggcaagcgcc tgaggcatat cactttcaag aagagtcagc
     1141 tcctgctcac gggtgatcgt gtcaaggtcg taatatacat agtcggtatc tgtgcggcgt
     1201 ttagcaacgt ctatgcagga ttctgcgcgg gcaagtatcg catcaatgtc atctgtactg
     1261 ccgtccaata ctacgatgcc ggcacgcatt gcgatctcgt aaacggtttg ctcttttccg
     1321 taaaagaagt ttatcggtat cttcgcaaag cttttgagat gatttttaag gtgcgatttc
     1381 tttaccataa gacagaagtt gtcactgcct gagcgactgc atatttcatc gttatacatc
     1441 atcttattaa gcattttcgc atactttgct attatctctg ttgtagcctg ataaccgaaa
     1501 atcgtatttt acgttttggc gatctatcaa atcagtaaat acaatctgtg acattcagat
     1561 gatcgagatt ttgaatcc
//
LOCUS       DAWAOG010000526         1578 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10297_length_1578_cov_1.835194, whole genome shotgun sequence.
ACCESSION   DAWAOG010000526 DAWAOG010000000
VERSION     DAWAOG010000526.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10297_length_1578_cov_1.835194"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    766..775
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1255..1264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          440 a          340 c          343 g          435 t
ORIGIN      
        1 gtcgcgcgac ttttcaaaag gtcggaattg tccggcggca cacttatcaa gtgttatgct
       61 ccgcactgca actttagccc gaccgtaagc gcaagtcacg ctgtcgcgcg acttttcaaa
      121 aggtcggaat tgtccggcgg cacacttatc aagcattatg ctccgcactg cagctttagc
      181 ccgaccgtaa gcgtaagtca cgctgtcgcg cgacttttca aaaggtcgga attgtccggc
      241 ggcacagccg cccttagaat ttgacggggt cggtggcgag gtatttcttg accataagag
      301 cgatcttgaa gatgatcgcg tgatcgaact ctcttaagtc aagacctgtg agcttcttta
      361 tcttgtccag tctgtaaacc agtgtatttc tgtgtacaaa gagctttctt gacgtttcgg
      421 aaacgttgag gttattctca aagaacctct ggatagtgaa taacgtttcg tggtcaagtg
      481 actcgataga acccttcttg aaaacctcct tcaggaatgt ttcgcacagg gtcgtgggca
      541 ggtgatatat aagacgtgcg ataccgaggt tatcatagct tacgatagtc ttgtccgtat
      601 cgaatacctt gcctacttca atagcgatct gcgcttcctt gaatgaacgc gcaagatcct
      661 ttatgcctgt aacgggagta ccgataccga catttacctt tgtaaagaac tctcccgaaa
      721 gcgtatccga gatggagcgc gcaagctttt cgagatcctt gttatnnnnn nnnnncccgc
      781 cttgatctcc ttgacaagta ctatttccgt ttcggtgata ttgaaaacga agtccttgtt
      841 cttatcgggg aaaaggttct gaatgacatc atatgcggaa acctcgtttg ccgacacgat
      901 gcttataagg aatacaacgc gactgatatc cgcattgaaa tgcagctctc ttgcctttag
      961 cgtgatatcg ccggggagaa cattatcgag aattatattc ttaacgaaat tatttctgtc
     1021 atacttttca tcatagtact gcttgatgct cgacagggaa attgcaagta ttgaggcata
     1081 ctttgccgcc tgatcgtccg taccttcgat gaacaccgca tagtcaggct ttacatgaac
     1141 gccgaaaggc ttatatgtat agccgtcacg aatgaataca tcgtgagagt cgccaagctc
     1201 aatagaaaag aagtcactgt tcgttcctat tctggaaagc tcactgcagg cgacnnnnnn
     1261 nnnntctcta tacagtctgt attatgatca ttatgcgtaa acccttgaaa aagggcataa
     1321 aacggctgaa aataatacac caaatatatt ttactagaaa aaccgaaaaa aatcaagggt
     1381 tttttagaca aaatgtaaaa attacatcaa aaatttttgc gttattgcaa aaccgactaa
     1441 atagtggtat actaaaattt atgtatggat agtgggcagc tatgccctgt accataataa
     1501 attacattcg gaaggactga ttcttatgat cctgaaaatt aaaaacgcaa agaacggtgt
     1561 gtatgcgcga atgtgcaa
//
LOCUS       DAWAOG010000527         1571 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10381_length_1571_cov_1.932718, whole genome shotgun sequence.
ACCESSION   DAWAOG010000527 DAWAOG010000000
VERSION     DAWAOG010000527.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10381_length_1571_cov_1.932718"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          412 a          352 c          356 g          451 t
ORIGIN      
        1 ctgtttatct ctgtcattca gaaattcctt tcaaaagagg atcatcttct cctcgcaaag
       61 ttttatgtat aaccgccttt cgcaggcgat aattatctat aattcagcta ctatataata
      121 acttaaaaac cgctcgatgt caacggtttc gggcgatttg taataatatt gtaacttttt
      181 taaaaaaatc ggtgttaatt taaataatat tggtatttct ttctataaaa tattttgtgg
      241 aagttgacaa agcgccgcca cggggttata cttgaataaa aattctgtgt ataaggtgat
      301 attatgttcg tcaaaaacaa acgacccaaa gatacaatgt cggtaaagga catgacacgg
      361 ctcgacaaac gaccgctgaa atacgttacc atgcgcgacc ccgaaaccta taaggaggta
      421 cggctcggtg aaaacggcgc agtgaatata atggacggcg attttgtgct tgtctgcctc
      481 ggcaaggacg taatgcgctg tgacctatca aaggtgcgtg taggcgagct tatgaacctg
      541 tcgggcataa cggtcaaggg cattgataaa ggaacgggta aagaaatgtc ggttatcgct
      601 tactttgcgg ataaattctg atttcagccg gttcttgctt tgtggataag atagtaacta
      661 ccgcccctgc tttacgcaga ggcggtagtt gtattctatc gttccaagca attaaaatca
      721 accgaaataa tcgatcttca tagccttctt gacctcgctc atagtctgag ccgcgcactg
      781 acgtgccttt tcagtaccct tcttcagcac atcgtaaaca tagtccatat tcttcgcaag
      841 ctcctcacgg cgcagtctta tcggctcaag ctcctcctgc attacgttgt tgaggaactt
      901 ctttatcttc atatcaccaa gaccgccgcg cttgtagtgc tccttcaact cgtcaaggtt
      961 cttataatcg ggcagataac gttcaaagtg ctcatctgtg cagaatgcgt caagataggt
     1021 gaacaccgtg ttaccctcga tatgaccggg gtcggatacc tgaatgtgga gcgggtcggt
     1081 aaacatactc attatctttg tcttgatgtc ggcaggacta tccgcaaggt aaatgcagtt
     1141 gccgagcgac ttgctcatct ttgccttgcc gtcagtaccg ggcagacgca tacaagcctc
     1201 gttttcggga agtaatattt ccggctcgac cagcacaggc tcgtagatgg agttgaactt
     1261 gtggactatc tccttagcct gctcgagcat aggcagctga tcctcgccta caggaacggt
     1321 agtagccttg aacgccgtaa tatcggcggt ctggcttatc ggatagcata agaagcccgc
     1381 aggtatgctt gcttcaaagt ttctcattgt aatttccgct tttacagtag ggttacgctg
     1441 taatcttgat actgtaacaa ggttcatata gtagaatgta agctcggtaa gctcgggtat
     1501 catcgactga atgaatatag ttgacttttc ggggtcaagt cctgccgcca tatagtcgag
     1561 tgctacctcg c
//
LOCUS       DAWAOG010000528         1567 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10421_length_1567_cov_1.974206, whole genome shotgun sequence.
ACCESSION   DAWAOG010000528 DAWAOG010000000
VERSION     DAWAOG010000528.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10421_length_1567_cov_1.974206"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          452 a          351 c          369 g          395 t
ORIGIN      
        1 gggtgatcag atctcccaga agggtatcgg caacggtatt tcaatgatac tgtttgcagg
       61 tatcatatca cgagttcccg atatggttat caatctgatc aagcagatct cggtagctcc
      121 gcagaactgg ttcttcgttc cgctgatact cgttatttat gttgtaatga tcgcattcgt
      181 aatccttatg acaaatgcag aaagacgtat tcccgtccag tacgctaaga gagtagtcgg
      241 cagaaagatg tacggcggtc agacaaacca cattccgata aaggttgcaa tggcaggcgt
      301 tatgcctatc atcttcgcaa tgtctatcat gtcactgcct gcaacgatcg gatacttctt
      361 cggtgtaact gcaacatcgg gccacgcatt ctggagcgac tttatagctc tgttcagcac
      421 gaacaacgtg gtttatgcaa tactttattt cttccttatc atcggcttca actacttcta
      481 tattgcaatg cagtataatc ctgtacagat agccaatgac cttaagaaga acaacggtgc
      541 tatcccgggt tatcgtccgg gcaagcccac atcggatttc atccacaact cactttcaaa
      601 ggttactctg gtaggtgcaa tattccttgg tatcatcgca atattcccta ttatattcca
      661 gaacctcaca ggcattacag gcctttcact cggtggtacg acaatactca tcgttgtagg
      721 cgttgctctt gaaacagtcc gtgcactcga aagccagatg atgatgcgtc atcataaggg
      781 cttcctcgaa taagagaaag gaacttatat gaatcttatt ttcttaggcg cacccggcgc
      841 aggtaaagga acacaggcag aagtagtctg cgaaaaactt ggtatacccg ctatatcaac
      901 aggaaatatg ctcagagaag ctgtaaagaa cggcacacct gccggtcttg cagcaaagga
      961 gtgtatggac agaggcgatc tcgttcccga tgaagtcgtt atcggcatct taaaggacag
     1021 aatcgctcag gatgatgcaa aaaacggttt cattcttgac ggtttcccga gaacagttgc
     1081 tcaggccgag gcacttgacg ctatgggcgt tcagatcgac aaggtaatcg agatctctgt
     1141 tcccgatgag aagatcatct caagactttc gggcagaaga gtctgcgaag gatgcggcgc
     1201 ttcttaccat gtagacttta agcctacaaa ggtagaaggc aagtgcaatc tttgcggcgc
     1261 taacgtagtt cagcgtatcg atgataagcc cgaaacggta gtcgcaagac tgaagaccta
     1321 ctatgaaaag actgcacctc tcaaggatta ctacatgaga aagggcaagc ttataaccgt
     1381 acagggtcag gaagagatcg cagaaacttc aaagcttacc cttgcggcag ttgaggcaaa
     1441 gtaatgattc aggttaaatc ggcaaaagaa attgaaataa tgaagaaagc caatcagata
     1501 gcggctgatg ctcttatcgt tgcgggcaat gcaataacgc ccggaatgag tacatgggag
     1561 cttgata
//
LOCUS       DAWAOG010000529         1557 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10528_length_1557_cov_2.034621, whole genome shotgun sequence.
ACCESSION   DAWAOG010000529 DAWAOG010000000
VERSION     DAWAOG010000529.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10528_length_1557_cov_2.034621"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          350 a          415 c          397 g          395 t
ORIGIN      
        1 accgggagtc ttgtcgggct gagaccatgt aaggcgcact gcatcatctg cgaacagttc
       61 agcttccaga tcatctaccg caggagcggg tgtgctgaca tttgacaggc tgtagccgat
      121 aaagacagtc ttgttgcctt cctcaccgta gaggtgacgg aaccactgtc ccagcgtcca
      181 gtcgaatgtg tactgcgtgt aaatgcttgc ggggattccc tcctcggcaa gagcaggacc
      241 gtcaatatca ttgactgcac cgatgcaccc ctgcgaggag gttttcgtgg tcgaaacgcc
      301 cgaaccgttg ctgtagctca gactggtttc aaaaccaaca tcatgcgaga atagtatacc
      361 atcgccgaat acaccgaagc tgatggagaa gttgaagtaa aatccgtgag aaatctcctc
      421 agaaaccgat ttgatattgc tctttgaata ggaaacctca ttcagacctc ccgcatagcc
      481 cagacggata gtgttctggc tgagctgctg agcgccgagt gcactgtatg cgccccagcc
      541 cagctgattg tacatataag ggttgccctc gttgccgttc agccagtttt cctcttcgtt
      601 gatctgctca aggggataat agcaggcttc gtctttggaa gtcttatccg actccttagt
      661 gatggcgcga ttgccctttt tctccatata ggagttgtat tcttcagcaa agctgttgta
      721 gtcgtcaatg ctcatctgct gatacaccgg ctgctgtggg atagacaggc ttattgccgt
      781 ttcgtccgac cactcgccgt tttcgtccag cacctcatag cagtaaatga ataccggcgt
      841 tcttgcaacc agtgccgtat tatacgcctg cgccttgacg gtcatggcgt attcggtttc
      901 cagtgcttcg gaaaagctct tgctccattc aagagaatag ccggcagaga tctccaactc
      961 aaaaggcgca aacagtccgc cgtagctgta ggacataccc acgctgtagg aaacattttt
     1021 tgaagaggac tgctccagct cgtagctttc ggttatagta tagtcagtct cgttattagc
     1081 cgagtcgccc agcgccgcaa agtagggtgc cgcctgcata accgccttga cctcggggtc
     1141 gctgtaggcg tagcttacgc ccttatatcg tgcgataacg ccgtcctggt cacggtcgca
     1201 ggcagtcagc agtacctgtg cgccgccgcc cttataaccg ccgtctgctt tgttcggacc
     1261 ccaatcggag agtgttgttt taaagccggt agccgcgccg taatcggcgg cagtatcgtt
     1321 ataagtggcg gaaatcaaac cttttttgaa agaataagta tcgtctccgt cccggcagga
     1381 agtcacaaat acgatttgct ctctgccggc attgttgccg tcaaaattac ctacagcaac
     1441 cgagcggatg aattcttcgt gcccgccgag cgcaccgtca aattgaaagc cgctcatttt
     1501 atagccctgg gaggcaaccg tgtttaattc gccggtagcg gtgttgtatg tatacag
//
LOCUS       DAWAOG010000530         1556 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10541_length_1556_cov_2.043304, whole genome shotgun sequence.
ACCESSION   DAWAOG010000530 DAWAOG010000000
VERSION     DAWAOG010000530.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10541_length_1556_cov_2.043304"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          499 a          289 c          298 g          470 t
ORIGIN      
        1 gttgtatgct ttttgttaac aatatgattt actcatttgc ttcgtattta gttatcgaag
       61 atgattatca aaattatgag aagataaaag cagtaattga aaagcatcaa ttagaaattg
      121 atatgacgca aactcttgat agtttgtaca gccaattgta ttcgagagcg tacaacacca
      181 tcaatttcac aagcaattgc agtaaagatc agatattact acaaatggcc gccgtttatg
      241 cttacaaaga taaagacgaa ttgagcattg tcgattttgt caaaaagaag tagttggtgc
      301 aggtgcgaca ccgtgtcgca cctagtatac gagtaacaga acgccaaaaa cgccctctgt
      361 cagagcaaca gctccggcag agggcgtttt tcatttctcc tcggaatagc tttcagaaaa
      421 aagaagtcag aaagcgtttt gtatatgttt tgcacaatat agctactaaa aaatcagcat
      481 ttttactata attttttgtg cctttaccat tgacaaaatt accaaaacac ggtataataa
      541 aatctaagga gtgaaaataa aaaacactcc tgcaggagta gggcattacc agtgccgtta
      601 ctcttatcct cagaagatgc atgaccatca atctgattta gcagaagtgg cataatctgt
      661 gtcctatatg tccatattat agcacagatc atatacataa tgcaagagtt ttcagattgt
      721 ttttgcgagc atctgaaagc tcttgttttt ttattgatta ttttcattaa gttcacgcag
      781 ctttttttgg cgtaaacgtt ctttcgcttc taaatgcttg ttttctaatt ttgtgacttc
      841 atccaaaggc aaagagaagt gtttgcaaaa ctcggacagt tttgatttat cattcgggca
      901 gggaccgttc atgtctctgc gaccgttaag gattgacgca caaccggttt cggatatgtt
      961 ccatatcgat gaaagtatct gttttctttc tcccggctca gtgaaatttc tttcctctaa
     1021 gattctttct aaatatgtcc gatgcaaagt aatggctaac ttcttcataa aatacacctc
     1081 ctgtatgatt tttaggtacg acacgatgtc gcaccttatg aaaggaaaaa caatgatata
     1141 tttcaactac accaacattc tgaacaggaa tctcaaatcg cagttcggaa ttgatatgac
     1201 agacagtgat tacaacgtac ggattgatgt ttattacgtt gatcaagatg atgttaccgg
     1261 gaatattgca atggtaacat cgggaacaat aagcatttca ccattcgata agacaaatag
     1321 cgcaatgtgc aaaaaagtcg attcgctttc atgtagcacg ttcagatact acatatttga
     1381 cagcgaatgc ggcgatgaca acagcaaaac agcactttgt atcatccttg atatagacca
     1441 taacgaggaa atcgacgatt ggaaagaact gattgaccct gaccttgact attggcacgg
     1501 gtggcgttca gatatgtgca acgcatcgag atacacgatt gatgaaaacg gtaata
//
LOCUS       DAWAOG010000531         1552 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10582_length_1552_cov_2.249833, whole genome shotgun sequence.
ACCESSION   DAWAOG010000531 DAWAOG010000000
VERSION     DAWAOG010000531.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10582_length_1552_cov_2.249833"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          470 a          362 c          348 g          372 t
ORIGIN      
        1 taacagaaag gaaaaaacga tatgtcagga cactcaaaat ggagcaccat caagagaaaa
       61 aaaggcgaaa aggacggcgc aagagctaag gtatttacca agatcagccg tgaaataatc
      121 gttgccataa aggaaggcgg cggagatccc aacaacaacg caaagctcgc tacacttatc
      181 cagaaggcaa agtcaaacaa tatccccaac gataacatcg acagacttat aaagaaggct
      241 gtaggcggcg gagaaaagaa cgactacgag aactgcgtat atgagggcta cggtcccaac
      301 ggcgttgcag ttatagttga ctgtcttaca gataacagaa acagaacagc cggtgagatc
      361 cgtcactact ttgataagtt cggcggcaat atgggaacaa gcggttgcgt atcgtttatg
      421 ttcagcttaa aaggcattat cgttctcgac aacgaagaag gcacgatcga tgaagataag
      481 gctatggagg atatccttga agcaggcgga gatgacttca gctttgaaga cggctgtgtt
      541 gagattacaa cagaccccaa ggaagttgaa aacgttgcaa atgcactgag agatatgggc
      601 tacacggtag tttctgcaga agcagagcag gttccctcta catacacaac tcttacagac
      661 gaagagcaga taaagaagat gggacttctc ctcgaaacac tcgatgacaa cgatgacgta
      721 cagaacgtat ggcataactg ggatatggac tgataacccg tcttacctga aacagatata
      781 aaaacacacc gtatggattt tcgcttccat acggtgtttc ttttatattt ttacttttaa
      841 taaagcggca taaacactct cattgcgttc tctcctctgt taccccatgt gtaataagga
      901 acagcgatcg caagagtatc ggtcgttttc ggctcatcaa agctgtacgg cttgccgttg
      961 tcgtcaaatc ttaccgcctc gaccttaagc tttgtaacac cgctgagctc attctggtca
     1021 ggctcgcata ccgttatctc gccgccgcgc ttaagtgcca gtgacagcac atcgccgtta
     1081 tccgcatcct cgaagcaata tacaagcgga cctcttctta ttgccgccat accgcttaca
     1141 gcagggatct taacagacgg atatacaaag tggggcgtat cggaaagcac aagctttgcc
     1201 tcatctccgt cctttaccgc aataataaca tagccgttta taggtttaag ctcctgcttt
     1261 tcgccgttaa gcgcaagtgt gaatgtatcg ctccagccgg gtattcttat tgcaagtctg
     1321 ccgcccttta taaccgaata atttacagtc atatcataag gataatttgt cttgcagaca
     1381 agctccatac cgttttcaaa ctttatctca ccgtctgcat acatatggca gaagctcata
     1441 tcgctgtttt cgccgtaagc atagcaaccg aacgatgtaa taagccttgc tacgttaggc
     1501 gggcaacaag cacaagcgta ccacttgggt cttacaggta agtcatgacg at
//
LOCUS       DAWAOG010000532         1547 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10623_length_1547_cov_2.099196, whole genome shotgun sequence.
ACCESSION   DAWAOG010000532 DAWAOG010000000
VERSION     DAWAOG010000532.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10623_length_1547_cov_2.099196"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          365 a          323 c          366 g          493 t
ORIGIN      
        1 ccgaggggaa ggggtttggg gttagggttt gagaataagc tacatttcta aaaaaacaga
       61 ctttttctac aacctgaaac atttttctca tcaaaaaaat attagcgggt tcgtgatgcg
      121 aacccgctaa tacagttatt tattatttag tatcctgact attgatatag ttcttttttg
      181 attatcctac tcaggagtta ccgcttgaga taagtccgtt gtaatattcg atctgcttgt
      241 tgatagaacc gatattagcc tcacgcatct gtgtccatga ctgaccggcg taagtttcgc
      301 tctggaggtc gaacagtgtg ttgccgagga tatccttgat tatctgaccg gttgtttcgc
      361 cgagtccgta aggttcatca acgactgcat tgaagtttga tacatccttg aagctgtaca
      421 ggaagtcgta ctgttcgtcg gtatacttct tgttcttcat gatactttcc ttggtagtag
      481 ccttaagatc ctcatctgtg ttgctcagac ggcagcagtt gatgaataca gaagcctgct
      541 tagcgttctt ggagcctgcg ggaacaagat agccgaatgc tgacattgag tagtaatagt
      601 tgtctgcttc tggatctctt gggatcggaa cgaagaagat gtcaagacta tcatccttct
      661 tcatcttttt tgcatagttt gttatgatcc attcgcccat gccctggaat gctgctgtac
      721 ccgaaatgat aggatcttca tctacgtcta taacgccttc ctggaacttt gcaagacctt
      781 ccttcttgag tgtctgcata aagttagcgg ctctgtcaac gtttgcattg ttgaagttgc
      841 ttatgagctt gccgtcatta ccgatttcaa taaggggtgt acctgttgta tcgataaatg
      901 catgggctgt ataagctgtt gcaccgtaaa gacctgttct tgcaccgtca gaatctgagt
      961 caatgaactt gcgtatgcag tctgtcattg tatcccatgt ccattcgccc ttgtcgtaaa
     1021 gctccttggg atcttctata ccgatctgct caaatatgcc gcggttgtag ataaggaagt
     1081 agggcgaaac gttgtatgcc caggggtagt aatagttctt accgttccat gtgtacttgt
     1141 ttatatactg ttccagtccg gaccactgag gggcagagag gtcgatatac tcatcaagag
     1201 gagtgtacat attttttgac atgataagcg ggaacgtgtt gtccgcatag tcaacaaggt
     1261 caggtgactc gcctgaagaa atagctgttg tcagcttttc aagaatctga agtgagccta
     1321 ctattgtgca cttgatagta catccgtagt gttcggagaa gtacttatat gcgggcttta
     1381 cgtcacctgc ggtcgtaata tcatagcaac cgagatactg aagctctgcg ggctcaacct
     1441 cttcgcctgc gtctatgctt atgtcgccta cactgacggg gttgtctatc tcgtcatcga
     1501 tagttgtttc tgtgctcgtg ttgctgttat cggaagctgt aggagct
//
LOCUS       DAWAOG010000533         1546 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10633_length_1546_cov_2.184440, whole genome shotgun sequence.
ACCESSION   DAWAOG010000533 DAWAOG010000000
VERSION     DAWAOG010000533.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10633_length_1546_cov_2.184440"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          385 a          372 c          328 g          461 t
ORIGIN      
        1 cttcaatatc catatcctca ggtattttat ccctgatgcg ctttatattc tctatatcga
       61 cctcacgggc aagcactact ctcttcgcac ccattttgta cagctcgttt gcggtaacat
      121 agtttactac tcccgcctga gtagaaacgt gtatctcaag gtcaggcact gtacgcttta
      181 taagcgcgat acagccgata tcacttacaa ttgcggcgtc tattccgcag tcgtatgctg
      241 tttttatgaa ctccgggaga agctcgatct cgtcgtttga cggaacagta ttgcaggtga
      301 ggtgtaccct tgcacctctc gcgtgagcat actcaacgcc ctttttcagc gtttcggggt
      361 cgaatttagc cgagcccgct ctcattccga acatagtgct tccgagatat acagcgtcac
      421 agccgtaatc tacggcggac ataagacatt ctaaatcacc cgcaggcgac agtaattcta
      481 acataacatt aacctttcat tctgaattat tttacggttc tattacaaca ttccgccgca
      541 gaaagctctg cggcggaatg tattcatcat ggttttaatc tgctattgcg tggtcaagac
      601 cgcacttacg gagattttct tcgtgctctg ctatagtttc ggcaaagtac atatccgttg
      661 tttcaggatc tgcgcagaaa taatagtagc ttgtttcggg cgcataaagc acagccttta
      721 tcgcatcaag accggggttg catataggac ctaccggtat gccgtcacac ttataggtgt
      781 tgtaggcatc aaacattttc tggtcatact gcgatgtcga aagatgcggc tttatatgtt
      841 catctacata atgcacagta acgtccgact caagatgtgg gaacgacccg gcatcagcca
      901 tacgattgaa gaataccgat gcaacgtttc ccatattcgc tgtagtatcc gcttcacgct
      961 gtgctattga agccagcgtt atggtttcat caagcgtaag acccagttcg gtcatacggt
     1021 tataataacg cgctgtgagc ttgttgttga agttctgata gataatatca agagcatccg
     1081 cggcatactt gcttgtatca agctccttgt tgtcttcaag agcaggaagg atataaaact
     1141 cataagtatc ggggaaaatg tagccctcca tctgataata cttcagcttc tcattttcaa
     1201 gccgcttctg gaacttatag gtgtcgccga gcttcttgca ctgctccata aagtcctcgg
     1261 cgcggcaaac aaggttatct tccatcatct gtgctatctc atcaacagtc ataccctcgg
     1321 ttatctgtat cgaaaccgtc ttgtttacag ttgaaacagt ctgtaatgtt gaaatgagtg
     1381 agctgtacga catagtcgta tcgaccgtga aagtgccctt tataaacttg tcgcctttgc
     1441 cgaacatatc agtgtacagc ttgaacacat ccggcatatt tatgataccg aggtttgcga
     1501 gattttgcgc gatttcctct gtcgtagagt cttcatcgat atgtat
//
LOCUS       DAWAOG010000534         1542 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10670_length_1542_cov_2.905851, whole genome shotgun sequence.
ACCESSION   DAWAOG010000534 DAWAOG010000000
VERSION     DAWAOG010000534.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10670_length_1542_cov_2.905851"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          440 a          394 c          296 g          412 t
ORIGIN      
        1 taagaattat gtacagcgcc gcaacaaata ccgtacctat gatagccaca aagtaaagtc
       61 ccgcgcctgc cgtgatacct gcgcctactc cccagaataa gaaacccaca tcaagcgggt
      121 cctttatcgc cgagcggaat cgcacgatag acaatgcgcc gaccataccg agcgacagta
      181 caaggttagc actgatagtc attatgataa acgcagtcac taccgtaata agcagtacaa
      241 gtatattgaa attatcgctg tagacgacac ctctgtagaa catacggtaa acaagataaa
      301 ttatagcacc gcagagtgta gccgtgagca gtgcgagcag tattctcggc agcgacaggc
      361 tttcaagctg tgccgtgata tcaaagctct gtcctataag ggagagaaga tcgtcccccg
      421 tgctttccgc aagtgcggtc agatgttgta acatttttct tttcctccga tcggttttcc
      481 catggcgtta cttaagtccg catattaaag cttgtcagct tatcatggca taataaaaat
      541 ttagataccg actcctgttt cagctcgata ccgccgagca gttcttcaag ataagacggc
      601 agatagtcgt catactttat ctctataacc gccgtgaaat cctcaacagg cagatatccg
      661 atattctccg acagcatatc atctgaaaac gctcctgctt taaacccgct gtcaatagta
      721 aacctcacgt ttccctcggg atttataaat gcctgcctgt tatagtccac tataaccgac
      781 gggcggagca gtgcaagcgc attgctgtaa gatgcgtcct caaggatagt gccctcatac
      841 tcaccgctcc atacaaaaga gtaatctccc tttaatatag aatgatactg tccgggactt
      901 atccatatgc cgcgttttga tgtcatatcc ctgtccttgt acttgcattc aaaatgcaac
      961 agcttctccg acaggtcata cgtccttatg cggtatttct ttcgggtatc cgcaccgatg
     1021 agcttatcgt tgtacgccga acggtaaaca tcatcaagat acacgctcgt tacacggtaa
     1081 cacccgttat cgccgtgagt atcaggtgtc attacagccg aaaaacgctc cctcagcact
     1141 tcacattgag cagtcgttgc cggaaactta tattcatgcc gcaatgtacg ttctctgtat
     1201 ttcataccgt ccctccccgc atattgaaat tcgcataaaa gaaatataag cagtatgtaa
     1261 tagtatagca tatcctaccg taaatttcaa cagattttct gcatatcttc ctttattttt
     1321 gttgtaatta tctgtaatgt aaaatccgca accctgaatg taataaactg tgaataatcg
     1381 cggcggcact ttcggcacat attgaaaact ctctccgcta atctctattt tccgcacgca
     1441 aaaaaaggat accgcaaacc tgaccggtcc acagtatcct cttataatgt ctgaacaaaa
     1501 atcgcagtat catattgtgt ccgccggtaa cagaggtcaa ac
//
LOCUS       DAWAOG010000535         1536 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10745_length_1536_cov_2.520594, whole genome shotgun sequence.
ACCESSION   DAWAOG010000535 DAWAOG010000000
VERSION     DAWAOG010000535.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10745_length_1536_cov_2.520594"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          382 a          381 c          312 g          461 t
ORIGIN      
        1 tgtcaaggca gagaacatct actcttgcga gcgtttccac gcagtataag tcctgcgcca
       61 gcgtcttgtt cgtagcaagt ctgataacgc ttacagcaag caccatactt gccataagca
      121 caagcccctc cggaatcatt ccgattatag ccgaaaccgt atttacaacg gcatcggtga
      181 acgtgttgtg tgcaagcagc atttccttac cgaacatcag cagtatcatc gggataatac
      241 acacagagat catcttcaat atatagttta tgcttgctag catctttgag ttattcttct
      301 tgatatattt tgcgccgctt gttatcttat tggcgtagtt gtctgcacct atgcggataa
      361 cctgcgcctt tgcgttaccg cttataagga acgaacccga cagtatctca tcgcccttgt
      421 gctttacgat aggatcgctt tcacctgtga taaggctctc gttcacctcg cagtcgccct
      481 ctaccgctat gcagtccgag cagacctgtc tgccggccga caaaatcatt atatcgtcaa
      541 gaacaatatc ctttacggct atagtctgta tttcgccgtt tctcagcaca tcagctttgg
      601 gcgcggaaat aagagccagc ttgtcgatgg ctctttttga gcgtatttcc tggaatatac
      661 ctattgccgt gttgcatatg ataacgccta agaacaggca gttcttgaac gaacctaccg
      721 caacgacaaa agccgccagt attatgttga taagattgaa gaatgtaagg cagttatcct
      781 tgaatatctg ctttacggat ttgctcggta tatcgaagtc accgttgacc tttccgcagg
      841 ctatgcgctc atttacttcc tctgcggtaa gtccggatat cacatctgtt acattttcaa
      901 gagaaacgtg ttcttcgggc attgacatat ataagttgtc cttccgtaat tattggtttt
      961 gcgttgtact gtcctgcaaa atactgtctg aactgtcatc agcctgagta tccgtatttc
     1021 tgatatagct tacatagtcc ttcagttcct gcgatatccc tttttcgccg tagatatcag
     1081 cggttatatg ctcaccgaac tcccagaacc ttttcatttc ggcggtgtta ggcataatat
     1141 aagagaaatc aagctggtca acaaatccgt caagcttttc gcttatattg atatttgtcg
     1201 ccgggagcgt acctgttatt tcggttctca ggctctgcat atcagtgctt agcagatatt
     1261 ttgcaaaagc gtccgcttct gccgggtgtt cactctttga atatacaaac atcgccctta
     1321 cacctgcaag agaatatgta tcgcttccgt tgtcaaatgc aggaagcggc actataccat
     1381 agtcaacacc ggatgcgtcc gcctttgtta taaaccacgg accgttcacg atcatagccg
     1441 cacccccgct taagaacata tcgtcaaaat catcatagct gtaatttgtt atatcctcag
     1501 ggaatatatc ctgcatctgc ttgagcagag taagcc
//
LOCUS       DAWAOG010000536         1531 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10796_length_1531_cov_2.043360, whole genome shotgun sequence.
ACCESSION   DAWAOG010000536 DAWAOG010000000
VERSION     DAWAOG010000536.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10796_length_1531_cov_2.043360"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          391 a          342 c          327 g          471 t
ORIGIN      
        1 agcctgctct gatagcggct tcagccagag cttcgttacc cttcataaga cttttctcag
       61 ccattgttgt cacctcctac agtgatagcg cagtcggggc acatcatagc gcacagtgca
      121 caagcgatgc aagcatcgtc atctgtgcat acggcggtgt agtagccctt cttgttgagc
      181 ttttccgtct gtaaggcaac tattttcttc ggacaggctg ttgtgcacag tccgcaacct
      241 ttacagcggt caaaatccac tttcattttt gccatattac tgacctttcc tttcgcctgt
      301 gattttttgt attcggctta ccattcaaat tcggtaagga aacagcttaa atctgttagt
      361 ccgcaaaatc ttcccatata cctttatagg atacgccttg tcgatcccgt caagaaattc
      421 cgcctttgcg gtcactgccg aaagcggaag acccgccgca cggcatactc tgtccgcata
      481 gtcgaccgag ccgtgtatta tgttactgtc ggtttcactg ccgaggtttg tgttgtttat
      541 tattccgtca cacttcatgc ccgatgcgtt ttctatctcg tacatcagct gtaatgcttc
      601 ttcgggctct ttcgtgagat atctgcaacg gttgatgacg tacagcatct gtgtgtcgta
      661 tttttcaaat acttttctgt atctgccgag tgctttagca ccctcatcgt cgccgccgac
      721 atctattata atatatccgt cacggtcggc aattccgttc atatcaaaac ccagcgccgg
      781 aatgtcaagg tttgagtttg catatgtcgg cacggtaagg ctgatattgt acttgccgaa
      841 cagctctttg aaatcggctg ttctgaaata aggatttacg atatccaggt caacgaccgt
      901 gaccttttcg ccttttgcgg caaggtcgag cgccatattg acagacacgt ttgttttacc
      961 cgaaccgtaa tgtcctgtta ttatatttat ctttttgaaa tccattttta tcctttttgc
     1021 actgtcccgc ttatttggcg ggtaaatcaa caaatcaatt ataacacttt aaattccgtt
     1081 tgtaaatagg caattttggt aatgtgcata aagcaggtgc aaagtaagcg gtcggtatta
     1141 gtcaaaatac agatattcgg cacagaagca taaagcgggc aaaagtgttt tgataattgt
     1201 cggcgggtct gcttttttga ctattaagcg aggcgcttgt caaaagaaac gttcatatga
     1261 cgatgtatgg tattgaaaga tgcggaagta taagtttgcg taaaaaatgc aaaatccccg
     1321 taatacggca ctgttcaacg gaaattgaca aatttcagac tgttgccgtt cttgacattc
     1381 ggacggttat gttgtaaaat ataaatcaaa tgcggctctg ccgtttaccc gggaaaatac
     1441 agctcggctg tagcatagcg agaaagcaat gatcggcaca atgaaacggg tacatgaata
     1501 acggatttat tttaagtaaa ttataataca a
//
LOCUS       DAWAOG010000537         1528 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10820_length_1528_cov_1.824847, whole genome shotgun sequence.
ACCESSION   DAWAOG010000537 DAWAOG010000000
VERSION     DAWAOG010000537.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10820_length_1528_cov_1.824847"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          365 a          373 c          301 g          489 t
ORIGIN      
        1 gccttaaggc tgtcctgtgc aatgagtttt aatgcgtcca ttttttcctc ctggttttcg
       61 gatattcttg tatcggctta tcctccgcgc attttctgcc tacggataac ggcaactttt
      121 gccgttaaat acagcggact acccgtttta atgtctttgt gcttttcgca ctaacggcat
      181 taaacacgct gacagtatat accaaaaggc ataattatcc catcagcgga tttcaaatgc
      241 tttaatagta tagcaccgtt atacgaaatt tgcaatagtt ttttgttaat tttttcggta
      301 actacgcctt atttcgcaca aaggtgctta taattgtaaa tttattcaaa ctgagtgccg
      361 tctttacata gtattccggt tcgtactata tcaagtcttt cagtttcctc gccgctgaat
      421 ttctcaaatg cgtcaaagaa aagcgacgga ttaagcgtaa agtcacagcc ggacggcatt
      481 ataagagtaa aatgtccgcg ttcgtaattc tttatctctg tgaacggctt gatatcaagc
      541 gtggttatgc cctttttggt cttcttctca acttcgacag acggcagctt cataaactct
      601 tcaagtttat cgtaatcaca tacgatattt accgaataca ccgacttttc tatctcggtg
      661 tgctttttta ccggcaatgc caccctgatt attctgatat ccggcggtaa aaccgcattg
      721 agcttgtcac gcaccgtatc aaaatctatc tgctcggtaa gcttgatatc catcgactca
      781 ttcacgccct cgcttccgag cgaaagcggc agattgacat tgacgtaagt gtgcggattg
      841 aagccctgcg tgtgccatat cgggatatcg gtacgtctta tcgcacgctg aaagcaggtg
      901 ataaggtcta gcgcagatat atattttgct ctgcccgtct ttgaaaagaa caaacggaca
      961 gcgatattat tctcattcat atttaaagca agcccttcct aagcatttgt ttatgccgca
     1021 gcctgcacac ttttcacggc agtttgcggt ggtcttttcc tcgtgcgcct tgatattctc
     1081 cctgacaagg aagctgcggt caaacagcat atccatatgc tcccacggag cgatctcatc
     1141 gtattcacgc ttacggttag cataaaatgc cttgtctatg ccgaatttct caaatgccgc
     1201 attccatatt tcgggcttga agtattcatt ccaaccgtct agcttacagc cgttcttcca
     1261 cgcctcatag ataacggcgg acagctttct gtcgcctctt gcgagcaccg cttcaagtat
     1321 agtcgtgtcg tagtggctcc agctgacatc gatacgtctt ctgccctttg agccgattat
     1381 atcaagcaga tatttctgcc tttcgtttat ctgctcctct gtcgcctgcg gttcgtattc
     1441 aaacggagta aacggctttg gtataaatgt tgacagcgat attgaaatgc ttatcgcctt
     1501 cttgcttctg ccctccgtat tataataa
//
LOCUS       DAWAOG010000538         1523 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10871_length_1523_cov_1.991826, whole genome shotgun sequence.
ACCESSION   DAWAOG010000538 DAWAOG010000000
VERSION     DAWAOG010000538.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10871_length_1523_cov_1.991826"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          379 a          294 c          402 g          448 t
ORIGIN      
        1 ggtatgatga tgcttccgcc cgctatgata tcgctgccgt tcaagataat acttttcgta
       61 ttggttgacg gctggcagct gatggtagga acgatcgtaa actcgtataa tttgtagctt
      121 aatgatcctg gaagggaggg cttgatttga cacaggatat agcaatggag gtctttaccg
      181 ccgcacttgt tcttgcgctt aaattgggac tccctgtttt gatagccgca gttgtgatag
      241 gacttataat agcgatatta caggcggcaa cgcaggtgca cgagcagacg ctctcgtttg
      301 cacctaaggc tatagtggta gcgatagtgc tgctcctttt cggtccgtgg atgatgaaca
      361 gctgtataga gtttttcaac tatatatttg agcttatgac aacggtcggt gtaacatgac
      421 gctgagtgac gtatgggata tgataatgaa taactttctc ggctttctgc tgataatgtg
      481 cagggtatcg ggaatattca gcttcaatcc gattttcaac cgttcaaatt ttcctgtaag
      541 actgagagcg ggcacgacga tagcactcgc cgtgcttttt gccgcctcta tgggagatat
      601 ccgatatgag ccgacaggca tatttatgat gctgtttgat atgtttaagg aaatagggct
      661 gggactgata ctcggcttta tggtaaacct tatacttaca gtaacaatct ccgcgggtga
      721 gcttatcgac tatcagacgg gtctttcgat ggcaagggct atggaccctg caacgggagt
      781 gtccatgccg ctgtttgcga atgtttatta ctatatgttc attctgtatt tctttttaac
      841 gaatgggcac ttatcttata taaagctgtt tcatatatcg tatgaaacgc tgccgatagg
      901 cttttcggga tttacggcgg acgtgccgtt ggcgcttgta acgtattttt caacggtgct
      961 tacgcttgca gtaaagcttg ccgcaccgat aatcgctatc gagtttatca ccgaaatatg
     1021 tcttggtgta ttgatgaaag cagtaccgac gatacacata tttgcgctga acatacagct
     1081 caaggtactt atcggtcttg cggcggtgct tataatggca cagcctatgt cggacttcat
     1141 cgaaaagctg atgggttata tgtggcagaa cctttacggg ctgctcggta tgatgggcgc
     1201 ttaaggaggg caaatggcag gcgaagaaaa aaccgaaaaa gccaccccga aaaagcgtaa
     1261 ggataccaga aaaaaaggcg aggtactgca aagcaaggaa gttgcggttg cggttttcgt
     1321 ggtgggaata ttcgcatttc ttgcgatatt cggaaactat atgtttcaga tgctgttaaa
     1381 ctttatggag cagtcgatgt catctataga caagacgtgc aataccgtgg agtttgcgat
     1441 gagcgtattt tggaaggtgg ctataatctg cgtatgtacc gtaggtccta ttctggcggt
     1501 gggagttgtg ctcagtgtac tgc
//
LOCUS       DAWAOG010000539         1514 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_10958_length_1514_cov_2.047293, whole genome shotgun sequence.
ACCESSION   DAWAOG010000539 DAWAOG010000000
VERSION     DAWAOG010000539.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10958_length_1514_cov_2.047293"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    459..468
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          361 a          342 c          327 g          474 t
ORIGIN      
        1 tataatgata tctttttatg aatttcaaac gttacgcaag cccacattaa gcacaccgcc
       61 gggtctacaa aaaatcaacc gacagcacaa ggcttattac aggcttccgt tattagaatt
      121 ttcttctgac agctgttctc cgaaagggca taattatccc tttgaagatc agtccgttgc
      181 ggataatatt ttattattcc gttcctgttt tccttgtatg tcggtcaatg tacttgcttt
      241 taattatcta tacatcgtct ttgctacttc agcgagtata tcaacgccct tttcgatctg
      301 ttcatcggtg ggtgttgaat agttcagtct gaacgacaga caaggagcat tttcatctgt
      361 taagaacgca ttgccgggaa ctacagccac cttgttctgt acagccttct tgcagaagta
      421 aggcatatct ccgtcaggca gagtgcccca gataaacann nnnnnnnncc tcgggctctg
      481 taaacttgat ggacttgggc atcttcatct ttaggttagt aagcataaga tccgacttgt
      541 gcttgtagat ggctctcagc ttttcaagat gctcttcaaa gttattctct gttacgaact
      601 tataagcgat catctgcggc aggatagagg tgtgaacggt tgaaacctgc atgcagacta
      661 ctgcctttgc gattacgttc tttggcgcgc agaggtatcc cacacgcaga ccgggtgcaa
      721 gcgtctttga gaatgttccc gcatagagga catgaccggt cttatcaaga gactttatgg
      781 taggtacgtc ctcgccgaaa taacgcagat cgccgtaagg attatcttca agtatatata
      841 cgttatactt ctgagccagc tcaagcacct tcttgcgctt ttcaaggctc atagtcttgc
      901 cggtggggtt ctggaagttc ggaataaggt agatgaaagc tgttctctga tacttttcaa
      961 gagcagcttc aagcttttcg atatttattc cgtcatcgtc catatctatg ccgacaagct
     1021 tagcattata ggaacggaat gcgttcagcg agccgataaa tgtaggtgct tcgcagataa
     1081 ttacgtcacc ctcgttgcat atctccttgg tgactacctc tatcgcctgc tgtgcgcccg
     1141 atgtgattac tacatcttct tcgctttcat caaacattcc ctttttctta aggtcgtcct
     1201 ttatccagtt gcgaagcggt gtatagccct ctgtaattga atactgaaga gcgtcaatgg
     1261 gcgtagttgt aagtatttcg tttgttatac gcgcgatctc ctctgtaggg aaagcctcgg
     1321 gtgcagggtt gcctgccgca aagctgatca cctcgggatc tgacgtaaac ttaagtatct
     1381 ctcttattgc tgatggctgc aatgtctgta ctctgtatga aaatgcgttc atatttatat
     1441 atcggtctta ccgccgcata aacggtatga cctttcctcc ttgcatttat tttgtaatgg
     1501 gtgagcggca tatt
//
LOCUS       DAWAOG010000540         1506 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_11035_length_1506_cov_2.202619, whole genome shotgun sequence.
ACCESSION   DAWAOG010000540 DAWAOG010000000
VERSION     DAWAOG010000540.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11035_length_1506_cov_2.202619"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    523..532
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          434 a          302 c          389 g          371 t
ORIGIN      
        1 ctttatgaca gggtaaagga ctataagatc gattttctgt ttaataatgc gggatttggt
       61 ctgtatggct ctttccttga aacgaacctt gatgatgagc tttctatgat caatgtcaat
      121 atcaaggccg ttcatatcct gactaagctc ttcctgcgtg atttcgcgaa gagaaacagc
      181 ggatatatcc ttaacaccgc gtctgtagca ggctttatgg caggacctct gcttgcgaca
      241 tactatgcga caaagaacta tgtcgtacag ctgacaaagg ctataaatga agagctcagg
      301 agcacgggca gcagagtagt gataagtgca ctttgcccgg gtcctgtaga taccgagttc
      361 aacagtgttg caggcgtaaa gagcttcggt atcccgagtg aaaatgaggc ttatgttgca
      421 gactatgcta ttgagcgcct tttatgcggc gatctgctga tcatcccggg cgttaccacc
      481 aaggcggctg cggcaggctc aagatttgcc ccgacaaagc tcnnnnnnnn nnctgtgaga
      541 acattacagg aacagaagaa caagtaaaga aagaaaaaat ataatgtgag gaactatggc
      601 atatactacc gattttgaga agctgaaaga acagctgaat tataaacccg atccgacaaa
      661 gcttgtttca aagcaggcaa gcgaaaacac cgtaaagctt tttgactatc ttaagagcat
      721 atatggcaaa aagcaccttg caggtcagca gtatctgcaa agcgacgagg tcgaggatct
      781 tgtctattac cgccttacag gcaagctccc ggctgtgaga ggttatgatt ttatgggagt
      841 aagctcctgt aagaaaaccg atgatcaggt tgacagagcc ataaagtggg ctaccgagtg
      901 cggcggtatt gttacaatgt gctggcactg gtacgctccg aatgatatga acgattactc
      961 caaaggctca tcgttctact acaagacgac cggctacgat cacaagacct gctttgatct
     1021 gtgcaagggt gtgacagagg gcacggagga atatgagttt attatccgtg agatagatat
     1081 ggtgtctgcc gagcttaaac gtatggctga tctggacata cctgtgctgt ggcgacctct
     1141 gcatgaagct aacggcaact ggttctggtg gggaaatcac gatgaaaagc accgcgaagc
     1201 ctataagaag ctgtggtata tgatatttga ccgtatggaa aactatcata agctgtctaa
     1261 ccttatatgg gtgtggaacg gtcaggacaa gtgtatggag gtagacccca acacattcga
     1321 tatctgcgga gatgatattt attccgtaaa ggaatatgac cattcttccc agaagaagag
     1381 atttgaatat atgacggagc ttgcacacgg caagctgata acattgtcag agtgcggcta
     1441 tattcctgaa cccgatgaga tgaaaaagga caatgccatg tggctgtggt ggcttccgtg
     1501 gtgggg
//
LOCUS       DAWAOG010000541         1506 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_11033_length_1506_cov_2.466575, whole genome shotgun sequence.
ACCESSION   DAWAOG010000541 DAWAOG010000000
VERSION     DAWAOG010000541.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11033_length_1506_cov_2.466575"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    841..850
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          375 a          397 c          332 g          392 t
ORIGIN      
        1 gtgcacgagc cttgcgtaag taaggctcag actctcacct agtccctttg gttctttccc
       61 tcgttcatag tagcaacgtt atcaattgga atagcgaacc cgagaagtgc tatcccacaa
      121 agagcactat cggcagtctg ctgttatagt ttattttcaa tcagaataaa attgtattca
      181 caagcaaagc cgcccgataa aacgagcggc tttctgtaaa caaaaaccga ccctttcggg
      241 tcggcataat atccatttgc aactttacct ataagccgag ttttgtcgtg cgcggtcatc
      301 tatctacgcc ctgcgtcgcc gcagacctcc agccacctta aatcgggccc gccgagcaag
      361 cggttgatcc ccgaaaccaa tcggtgttgc atcggatagg gtttacaggc gggatcagtt
      421 gcctgtcccg cggtgagctc ttacctcgcc tttccaccct tacactgtaa aaacaatgcg
      481 gtatatttct gttgcactcg ccctggagtc acctccggct gccgttaaca gctaccctgc
      541 tctgtgatgc tcggactttc ctcacggttt acaccccgcg accgcacagt aaagttgcga
      601 tagttattgt agcatatttt tttataaaaa tcaagatggt tttacaaacg ataggtttgt
      661 agaaaaagcg taattttcaa tgtgcagagc ctttggtcat acacgcccgg catccgtgcc
      721 ggggctgagt gtggggctgc cgccccacca agcaaggacg cttgaagtca gtcgcacaca
      781 gtcgctatgc aaggacgcat aaagacagcc acacaaagcc gttgcacgat gcaacggcac
      841 nnnnnnnnnn gctacgcccc aaacccctat tacagaaagg agcacaagcc tatgtcaaaa
      901 aagcagacca taaaaaagac attcaatata acatggctgt tcctctacgg tgcaggactt
      961 atcgcggttg gtatacttgt gcttgtaaac ggaaaaatag tggtagagcc tgccgcaagg
     1021 ctcggcggca tattcattgc cgctaacggc attcaccgcc ttatccgcgc atatgcaaga
     1081 catcaaaagc tccctgtatt cagcggtata agcaatattt tgatcggtct gatatcgtta
     1141 ttctttcccg ccgcaacatt agccctgctg tcgtttatat tctcgcttta cgtttttctc
     1201 aacgcacttg tcaaatttat cgacttcggt tcggcgctta aaaatgccgt tcccgatgca
     1261 ttctatgatc tcttttcagg gatattcttc accgtgttcg gtataattat gatgttcggt
     1321 actcttatgg gcagtcaggg tatgctgata gtaatcggaa tatactgcat tatctatggc
     1381 gcgggcgaac tgcgcctgtt tatccgggaa gctgctccga acaaagcaaa aggcatgatc
     1441 cgccgcagga tacgtttctc gcttccgcag atcataacca cgttcatacc gctgaagacc
     1501 ctgcgc
//
LOCUS       DAWAOG010000542         1504 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_11058_length_1504_cov_2.817115, whole genome shotgun sequence.
ACCESSION   DAWAOG010000542 DAWAOG010000000
VERSION     DAWAOG010000542.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11058_length_1504_cov_2.817115"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          487 a          282 c          348 g          387 t
ORIGIN      
        1 ataacgatac cggacggtgt gacgagtata ggtgatggcg ctttctataa ttgctcaagc
       61 ttgacagaaa ttaaagttgc atctgaaaat tcaaattatg tttctgtaaa tggcgttctg
      121 tacaataaag ataaaactac tataatatgt tatcctgccg ggaaaaaggg taataattat
      181 aagatacctg acggtgtgac agagataggc agtattgctt tcagccgttg ttcgagtctt
      241 acaagcgtaa cgataccgaa cagtgttacg agtataggct ccggtgcttt caacggttgc
      301 acaagcctta ctagagtaac gataccgaag agtgtgacga agataggctg gaatgctttc
      361 agcgattgca caagccttac aagcataacg ataccgaaca gtgtgataag tatagactgg
      421 tatgctttca ggggttgtac aagccttaaa agtataacaa taccaaacag tgtgacaagt
      481 ataggcaaga atgctttcgg ttactattat gataacggct acaagaagat aaacaacatc
      541 aaaatatact gctacagtag cacagccggc gaacaatatg ccaaagataa cggctttgat
      601 tatatgctga ttgacaagtt tgcacatgca aaagtaaacg gcgctaagct cggtggcaga
      661 gccgcagacg cactgagaat aaactggact aagaatgcaa gtgctgacgg atatattgtt
      721 gaaatgtatc agaacggcaa gtgggcgcgt gtcggcaaga taacaaacaa cagcacgact
      781 acattcagaa aagcagggct taaggcttcg actgtatata agttcagagt gagagcctat
      841 aagatgagcg gtaagaccgc gctttatggc aattatagcg caactgtgac tgcaaggaca
      901 aatccgtcaa tagtaaaggg agttaaaatt ggcggcaagg ctaaggacgc gctcagagtg
      961 aactggacta aaaacacctc cgcacagggt tacatagtag aaatgtataa gggcggaaag
     1021 tggataagag ttgctaagat aacaaacggc aacactacta ctttcagaaa gtccggactt
     1081 gctaagaaca caacgtataa gttcagggtt tgcgcttatt atatgagcgg taagacggcg
     1141 ctttacggta attacggtag agtgagcgga aagactgcgg cgaagtaaaa atatcacaga
     1201 ataataaaag agtgcatcgg ttgccgccgg tgcactcttt tatgttattc cgtgatataa
     1261 ttccgcaaaa acgcgtcaca tatgcaattt atataattat cccactcaga tgatcgatct
     1321 catgctgaat gatctgcgcg gtgaaaccgt cataggtttt tatctttatc ttgaaatcct
     1381 tatcaagata tctgactttt atcttcctgt atcgtttcgt ttttcgtaca ccgtcaagcg
     1441 aaaggcaacc ctcttcggtt tcgtactcct tgtcggcttt taatatttca gggttataca
     1501 taac
//
LOCUS       DAWAOG010000543         1503 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_11070_length_1503_cov_2.196823, whole genome shotgun sequence.
ACCESSION   DAWAOG010000543 DAWAOG010000000
VERSION     DAWAOG010000543.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11070_length_1503_cov_2.196823"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          374 a          365 c          317 g          447 t
ORIGIN      
        1 gatatatccc tcgccgcatt ttccgaaagc gtcaccgggg acaacagcga ctctatgctt
       61 ttctatcagc tgttcgcaaa attcctgtga cgaaagccct gttttcctta ttgacgggaa
      121 gatatagaac gcgccctccg gttcaaagca atcaaggccc atttcattga agccttttac
      181 gataagccgt cttctcatat cgtattcgtt acgcatatat tcaatgtctt ttttgcagtc
      241 tttcagtgct actactgccg cgtactggct cattgtaggc gaacacatta tgccgtactg
      301 atggatcttg agcatctggg taacaatagg cgcgggacct gcggcaaaac cgagtcgcca
      361 accggtcatt gaaaacgctt ttgagaaacc atttataaga acggtgcgtt ctttcatacc
      421 gtcaatatcg acaatagaac agtgctcttc gttgccataa gtaagctcgg aatatatctc
      481 gtcggaaata acgattatat ctgtgcctct tatcacttca gcaattgcct caaggtcgga
      541 ttttctcata accgcaccag tgggattatt agggtaggga agtacgagag cctttgtttt
      601 cggtgttata tgtgctttca gagcttctgc tgtcagcttg aagccgtttt caacagtagt
      661 ttctatagca acaggtgtgc cgtttgcgag cgttacgata gggccgtaac agacaaagct
      721 tggctgaggg ataagtacct catcacccgt atcaatgagt gctcttatcg caaggtctat
      781 cgcttccgat ccgccgaccg ttacaagtat ctcgtgcata gggtcatagc tgacgtgcag
      841 tctgtcattg atatagtcgt tgatagcaat acgaaggtcg gcaagtcctg cgtttgcggt
      901 atagtttgta cgtccttttt caagaacttt tattgcttct tttcttattg cccatggcgt
      961 cttaaaatcc ggctcaccga ccgataaaga tataacatcg tcatagcttg cggctatgtc
     1021 gaaaaacttt cttatacccg aaaactgcag atcctgcacc tttcgggaaa gtatattttc
     1081 ataatccatc acggagatac caaactcctt tcatctgtgt tctttaagaa catcttgtct
     1141 ttttccttat atctatgcag aatgaaatgt gtcgttgtgc tgaggacacc gcctaaaggc
     1201 gctagtctgt ccgttacaaa catagctaca tcacgcagat ttgtgccctt tatagttacg
     1261 gcaaggtcat aaccgccgct catcaggtac accgatgaaa cctcatcgta ttgagctaca
     1321 actgcggcta catcgtcata accgcattgt atctcgggag aaactctgac ttcgataaat
     1381 gcgcagacgg agttcctgtc aaattcttct tcatttataa ttgcggtata gccgattatc
     1441 attccgctct tttccagctc cgctatcatc gccgcgacat catcggccga ggtgtcaagc
     1501 atc
//
LOCUS       DAWAOG010000544         1503 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            NODE_11068_length_1503_cov_2.274862, whole genome shotgun sequence.
ACCESSION   DAWAOG010000544 DAWAOG010000000
VERSION     DAWAOG010000544.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11068_length_1503_cov_2.274862"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          367 a          388 c          405 g          343 t
ORIGIN      
        1 ttgcccgcgg tgtgtcggta tattcctgcg ccgccgctgt cggcaaaatg gaaagcgacg
       61 gtccgctcgg caactgcttt gactatgccg ccgagggggc tgagctagga cttaccacat
      121 gggaaaaggc agagagcacc tttcagcaat atgcattcga tatagcgctc agaaagggca
      181 agcttataaa agaagatatt tcattcattt tcgcaggcga tctgctcaac cagtgcacag
      241 gctcggcata cggactgcgc gacaccgata taccgtttat agggctttac ggtgcttgct
      301 caacaatggc ggaaagcctc gccatggctt cgctttttgc cgatatgcga ctgggcgaat
      361 tttttgcggc ggtcacctct tcccacttct gctctgccga aagacagttc agatttccca
      421 taaactacgg cggtgtacgt ccgccaacag cacagtggac agccaccggc gcaggctgct
      481 gtataacctc tgccgccgaa aagccgccgt ttgtgaaaag catcaccata ggaaagataa
      541 ccgatatggg catcaaggac gcaaacaata tgggcgcggc tatggcaggc gcggcatacg
      601 acacgataaa acggcacttt ttagccaccg atacctctcc tgccgattat gacatgattt
      661 acacaggtga cttaggacag gtcggatctg accttatgca cgagcttttc cgccgtgacg
      721 gcatcggaat agaggacaag cacaaggact gcggacttat gctgtacgac cgcgaaaagc
      781 aggacgtaca cgcaggcggt tcgggttgcg gctgcagtgc ctctgtgctg tgcggacata
      841 tcctgccgca gatagcggac ggaaagctcc acagagtgct ctttgccgct accggtgcgc
      901 ttatgtcgac cacaaaagtg caacagggcg aaagcatacc cggcatatct cacttagtag
      961 agattactga cacaatataa caggagaaaa attatgcaat atttatgggc atttttagtc
     1021 ggcggtgcac tttgtgctat aggtcagctg cttatcgatc ttacaaagct aacccccgca
     1081 agaatacttg tagcctacgt tgtttccggt gtaattctcg gagcgacagg catttatcag
     1141 ccgctcgtcg actttgcggg tgcaggtgcg tccgtacctc ttacgggttt tggcaatctg
     1201 cttgcaaagg gtatgagaaa agctgtggaa gatgacggct tactcggaat cttcacgggc
     1261 ggatttaccg cctgctcggc aggtgttacc gccgctatga tcttcgctct cgcggtatca
     1321 ctgattttca acagaaccga caggacaagg caggaataat ctatcatcat tctgaccata
     1381 aacctatatt ttttagaaat ggggtttggg gcgtagcccc aagcggggtg agggcggcag
     1441 ccccacactc agccccggca cggatgccgg gcgtgtatgc acaagggctt tgcacgatgc
     1501 aaa
//
LOCUS       DAWAOG010000545         1497 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_16016_length_1497_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000545 DAWAOG010000000
VERSION     DAWAOG010000545.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_16016_length_1497_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          473 a          306 c          398 g          320 t
ORIGIN      
        1 atcgaagaaa tccgcgcggc gaaagaaatt ctgaaaagcc ttggtctgcg ccaattcggt
       61 ataaccttcg tttcctgccc gacctgcggc agaacaggta tcgaccttat atcaatagca
      121 aagcaggtcg aggagcgtac aagagatata accgccgata taaagatagc tgtaatgggg
      181 tgtgtcgtga acggccccgg agaagcgaga gaagccgata tcggcattgc aggcgcaaag
      241 ggtgagggta taatcttcaa aaaaggcgaa gttatccgca gagtgccgga agaaaagctc
      301 gttgatgagc tgatgagtga aataaacaag actgtaaacg gacaaggaaa gtgaaataat
      361 gtcggcgatt ttaaaagaac tactgaaaaa tgttgaactg ccgcagcaga caggtaatgc
      421 cggactgctg tcgatactgt gtgatgaagc agataaaagc atgatattac agcttgaagc
      481 tgatgagctg ataccgtaca gtgtactcgc cgaaaccggt aacattataa aaaagtatct
      541 gggtgtgccc tcggtaagga tatatccgaa atatgcacct gagctgttca atccgtcata
      601 tctttatgat attattcaga tactgaagcc gtcacacggt gtgataaacg gctatatgga
      661 cgattccgaa ataagcgatg acggagatac gttcgagttt acgcttatgc acggtggaat
      721 tgatctttta tacagcgaga agatagacaa ggaaatagag aagttcacaa agggtgtgtt
      781 ttctaaggct gtcacggtaa aattcagcga gaatgcaaca gtcggttatg ccgatctcga
      841 tgaaagatat tatgaagagc ttgccgcaca gccgctcccc gactttgagg agatagacag
      901 aaaagcggca gagcgtgcaa gcgagagcaa aagctccaga ggtaagaaat caagacctga
      961 gcctagaaag cgtcccggca aaataacgct tacatacctt gaggataagc tgaaccccga
     1021 cggactttta tatctcggaa acgagatatc ggaagctccg cagtcgctga aatcactcag
     1081 cgaagagagc gacagcgtta gcatatgggg tgagcttacc gaagttgagc acaaggaaac
     1141 gcgtaacgga gtatatacga taatcacggc ggcactgtgc gatgatacgg gaagagtacc
     1201 ggtaaagctg tttgcaaagg ctgagataat agatgattat tctttccttg aagacggcgc
     1261 ggtgtttgtt atgcgcggaa gctataagct tgatacattc gcaaacgaga tgcaattcaa
     1321 cccgacagat ataatgcagg tatcgctccg tgacgactat acgatgaaaa cgcccgctcc
     1381 gaagccgtcg gcacagggcg gaaatgcgtc tgccactcct gccatgacaa aggctaacgt
     1441 atttgccgag cccgaggaaa tggagcttat gtttgaaacg acccatttcg gaagcaa
//
LOCUS       DAWAOG010000546         1357 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_30157_length_1357_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000546 DAWAOG010000000
VERSION     DAWAOG010000546.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_30157_length_1357_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          365 a          282 c          327 g          383 t
ORIGIN      
        1 atataatact tgacgagcag gaggacgagg aaaagctgat aaagacgctc aaaatggcag
       61 gcttatttga agatgcaatg aagcttgaaa acaagcttga cacgacaata ttcaaaagct
      121 atgatgaaaa cggcactgaa ctttcgggcg gtcagcagca gaaaaccgct atctgccgtg
      181 ctttataccg tgacagcccg atagtaatac ttgacgagcc tacagcggca ctcgaccctg
      241 tagccgaata tgagatatac aagcacttca acgagcttgt aggcggcaag accgctatct
      301 acatatcgca taggctttca agctgtaaat tctgcgatga aatcgccgta tttgccgatg
      361 atacgataaa agagtacggt acgcacgatg agcttgtgaa aatcgaaaac ggtatttatt
      421 ccgaaatgta caacgctcag gcacagtact atgttgagaa cgcttcgtga tattatgtga
      481 tcttgtagag atattcgttg ccgagcttac cggcatagtc gatcacgccg acatacagca
      541 atattcttat cgcaatagag gcatcaccgg aacgcatacg ctttttaaac tctttgagcg
      601 taaacgtttc gggtaagcct tcggggataa agcatctgta gctgtcgctg tcctcaagat
      661 aagtaagctg tagcagtgag gtcggaacgg taatctttct gtccgacttc ctgcgtttca
      721 tatctttagc acagtatctc agattttcta ccgcaatatc tgcaatgcag atcgtgaggt
      781 tgggattgct gagatactgt tttattctgt aaatctcaat aaagaaatcg ctcatatcgc
      841 ttgcggtgac tgttcttccg cttttgagaa tttcaccggt ggatttgtca atgtaattaa
      901 gacggctttt tttatgatat gggtatacta ccgtgacgtg agaagctttg agaaaagtat
      961 tgagctttgg cacaaggtat ttgaaatttg ccgtctgtat ctcaaatatg ccgttctcgg
     1021 tgacaaggtc ggcaaagtaa ttgcctatct ttatttcctt gtcgaacagg gaagaggcaa
     1081 agtagctttt aagtgttgcg tgtatctgct tttcgttcaa aatcccgact ccgtcgccgc
     1141 tgtctgctgt agcggtgcag gcactgaaac gcgcgttgtc gggggcaacg tgtagtggtt
     1201 gtttattgta ctgccatacc gtgcagttat cattttgaat gaatgccgga actccgcttt
     1261 gttcgttgga gcggataact cctgttgcaa gcgctttgag tctgtcggtg gcatttgata
     1321 cagcgtaaca cctgtcggcg accgaaaaca tcgaaat
//
LOCUS       DAWAOG010000547         1352 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_1929_length_1352_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000547 DAWAOG010000000
VERSION     DAWAOG010000547.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_1929_length_1352_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          350 a          304 c          270 g          428 t
ORIGIN      
        1 attaaacaac aaattttttc atataatatc ctcctgtttg tgtaaattaa gtaacatttt
       61 gaattatatc attgctgcct gtttttgtca acgagtgaaa cgtttacttt tgtaaattct
      121 acaaaagtgc gctgtaaagt aagttaaagc aggttaggtt gtaacaattg tctaatgcaa
      181 ctttatcatt acacgatttg ttaactaaat tcagcataaa cataggtgaa agtttcacaa
      241 gcggtattgg atttcggttt gttaaaacaa ctgcccctac acttgcaggg gcgaatttca
      301 tatttccttg aaattaaagt ttacatttac gctgtccatc gtcttactgc gtctgaacct
      361 tgtcataata cgttcctcaa tgccgttaaa tgagctttca tcggcttcaa gcaaaagcag
      421 aagatactga cttctgctgt agcgtgtata aacatctctc attctgagtg agttgctgat
      481 gctgaacgat atcttatcca tcgccttttc gagcagcttg atatcctcaa gctcatttcc
      541 gctattatca acagcggaca caagacatat ggacagcttg ctttcaaggc gcatactttc
      601 acgcttgtac aggcggaata tatatttgaa tatttcgtac tggcagtaga aaacgccttt
      661 gcgttccttg ctgtcatcgc ccgacaggtc accgataacg gcatcaagat tattctgtac
      721 atcgtttctt ggctttaccg taagctcgta aagctcggtg aggtgtgcgc ccggagcggc
      781 accgtactgc tccataagat atgtgcgaat ataattatat tgctttgcgg cacgctcata
      841 ttcacccatc gccacaaatg cgcggataag atttgcgtgc attttctcgt ccgactggtc
      901 aagcatcgtt gcgcgttcgc acagtgtaat tatctcggag aattttccca tgggatacaa
      961 aagctgtgca catttgtcaa cggctgagtt atacaatgca tgaaaataca cgtttatcgg
     1021 ctgtacccat gtttcgcctg ccgtcacggc aaggtagccg ctgttataaa gcgatatcgc
     1081 ctttttgtac atacttatgc gtcttgttct tgaaagcgat tcgtcttctg ctttgttgat
     1141 gtattccttg aacaaagaag aatctatcac acattcattg ctttcgataa aggcgtatga
     1201 gctgtgactt cccgctatca gcctgccgcc gtccggtatc atatcatcaa gcaccgcccg
     1261 cgtcctgtga acaagggttt tcagcgcatt ggcgggattt ttgctgtccg accacaaaag
     1321 atcgataagc tcattctgcg ttatctctct tc
//
LOCUS       DAWAOG010000548         1346 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_43682_length_1346_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000548 DAWAOG010000000
VERSION     DAWAOG010000548.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_43682_length_1346_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          400 a          251 c          355 g          340 t
ORIGIN      
        1 cgtatgctca cgacctggga ctggcatttc agatagttga cgacatactt gatgtgacat
       61 ctacttcaga agaactcggt aagcctgtag gaagtgatga agagcagaac aagacgacaa
      121 gcattacatt gctcggactt gaaaaagcga aagagcttgc cgaaaagtat acgcatggtg
      181 cggaaaatgc acttgcggaa tttgacgata cagaatttct gaaaaaactg acggatctgc
      241 ttttaagcag aaagaaatga taagggtatt tataccttga ggagttcaat ataaatgaga
      301 actgatcaga taaatgtaat tttacttatt gcgcttataa gctggggcac tgcgcagata
      361 ataaagacat tcatacattt tatcaagacg aagagcttta aggcagaacg tcttacgggt
      421 gccggcggta tgccgtcggc acattcggct actgtctgtg ctacagcgat aacaacttgc
      481 agagtatgcg gcatatgctc accggagttt gcacttgcaa tgatacttgc aatggtcgta
      541 atgtatgatg ctatgggtgt cagacgctcg gcggggcttc aggcaaagga gatcaaccgt
      601 ttaaggcgtg tggtaaatga gcttgatgaa gagtttatgg ataaattcga cgacaaggtc
      661 gatgagatag aagaaaagaa gcccgaagag attcatgaac tcaaggagtt tttaggtcac
      721 actcctcttg aggttctttg cggtgctttg ctcggaatac ttatagctat ggctgtaccg
      781 atacaggtgg tcggctgaca gtacatcaat caggtgaatt aaattgatat tgaaaaacga
      841 tatgtccgcc gagtacctga agtcgcttaa ccctgaacag cgtaaaaagc tgtgttcgga
      901 gataaggcag cttcttgtaa aaacagtaac agtaaacggc ggtcatcttg cgtccaatct
      961 cggaacagtc gagcttacgg tggcaatgca cacggttttt gactctccga aggataaatt
     1021 tgtttttgat gtgggacatc aatcctacac tcataagata ataacaggca gagcaggaaa
     1081 aatgaataca ctgcgctgtg aaggcgggct gtcaggcttt cctaaagcgg aagagtcgga
     1141 gcacgatgct tttatcggcg gtcacagcag tatttctatt tctgcggcgc tcggaattgc
     1201 aaagggtatg cagcttaacg gcgacgatca cagcgtggtc gcggttatcg gtgacggtgc
     1261 gtttaccggc ggcgaagcat atgagggtat gaataatgcg ggtaaaagtg actgcaatct
     1321 tatagttgtt ctaaacgaca atgaga
//
LOCUS       DAWAOG010000549         1336 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_37545_length_1336_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000549 DAWAOG010000000
VERSION     DAWAOG010000549.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_37545_length_1336_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          304 a          289 c          281 g          462 t
ORIGIN      
        1 gacgaacgat gagctttaat gtttcaagac cgaaatagtc atcgatgccg cgaccgttga
       61 ttgttataac accgctgccg ggataaacac gtactctggc aactgagtgc tttcttctac
      121 ctgttccgta gtaataaggc tttgattcgt acatttttat ccctccttaa tatttgaatt
      181 cttcgggctt ctgagcgccg ttcttgtgct cagcgcctgc ataaagacgc attcttgtag
      241 cggcttttct accgagtgtg ttgttgggga gcataccctc gatagcgagc tgcatagcct
      301 tttcgggctt ttcgctcatg agcttgctgt actgtacttc cttaagaccg ccgatgtagc
      361 cggtgtgcca tctgtagtac ttgttctcaa gcttcttgcc tgttaaaact gcctttgcgc
      421 agttaattac gattacatga tcgccacagt cacagtgagg tgcgtacgtg ggcttgtgct
      481 taccgcggag aatgtgagcg gcctttgcag caacacgacc gagaggcttt cctgctgcgt
      541 cgagaatata ccacttacgt tcaactgttt cagctttagg cataaatgtt gacattctga
      601 ttctttcctt tcaattactc ttgaggtttt gcctcgtttg attattttca caacgttcta
      661 tattatagtc aaacaccgtc cgtttgtcaa acgatttttg agttaaaatc taaatatata
      721 tctatatctc tttaaatcgc tttaattatc gctgtttctc tctatttctc atctgtgatt
      781 tcactttgct tttgcgtgat gcaaataagc agtgttttcg gcagttttta ttctatttct
      841 ttcgcttttg cattgtggct cttttaccgt ttcaattttg attatcggca gaaaagtttg
      901 cttttatgcg tttaatgatg ccttagttat ctctgtccat tttgatatta ctgcgtattt
      961 tctgtgttct tttcttgcag aaattgcgct tttgtgctat gcttatgtta tcaaattcat
     1021 tataaggagc gatgaggaat gacacagtac cataatattc cgcccgtata tgataaaaac
     1081 agcaaaattc tgatattagg ctctttcccg tctgtgaaat cgcgggaagc gcagttcttc
     1141 tacggtcatc cgcaaaaccg tttctggaaa gtgctgtccg ctgttctcgg ctgtgaatgt
     1201 cctgtcacca cagaagagaa gaaggcgatg ctgttaagtc ataacatagc tgtgtgggac
     1261 gttataggat catgtgaaat aacaggctcg tcagatgctt caatacgcgc cgtagtccct
     1321 aacgatatag cgggac
//
LOCUS       DAWAOG010000550         1325 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_16431_length_1325_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000550 DAWAOG010000000
VERSION     DAWAOG010000550.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_16431_length_1325_cov_3.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          315 a          301 c          306 g          403 t
ORIGIN      
        1 cgctaaagga gaaacaatgt cttcgccatc ggtgctttta tatgtccact cgtttgttga
       61 catggttttg taatcagatt gaataaagcc attgtttttg ttgttcagaa gcgttgtata
      121 ggcaagatag ctttcatctg tttcaccgta accatcgatt tcagccttaa tacctgcaac
      181 aacaatgtcg tcgtaaccgt cattgttgat gtcacctacc gttatattgc aggcgttcat
      241 gctgcttgac gactgtactt ctccgtcgga gagcttgccc tcggggcaga catagaaaga
      301 ttcatctgct ttctgattga taaaagagcc ttccttcggc atagtgccgc cgtatttgat
      361 ttttacctgc gggcggtata catttttgtt gtcgctcgtt atgcagttta catggaaata
      421 cgaaagcacg gcaaggtcat ctatattgtc gccgttgaaa tcaccgacca ccatttttac
      481 gctcagcttg tgcatggcgt tatacaatgt atcttgtttt gagccgccga tgctgtcgtt
      541 tcttgcacct tttaccgatt ttccgctttg gtatgtctca atatagaggt cgtgaagcag
      601 gtcatagccc aaagatctgc catcacctgt ggttgtatta ttgtgattac tagtcaactt
      661 attgccgtca gcatcgtaac gcaattccca gaggagacta ccgacattat ttatatcttg
      721 gtaaaggtcg gtagtaaccc atgttgccga cttttttgtg ccgggtacat agacaataac
      781 ggtttccttg ccatcattat cgaagtcgcc ggcgacgaca tcaagaagtg cgtttgtctc
      841 atagccgttg acctgaaagt ctccgtcgga atctgcgatc cagtcgaggg gaaaattaat
      901 aatataggtg ccgcactgct tacccgttac ggtattggac aaaatggcat aaacttgttt
      961 gcgtgttctg tgaaccccga catagacaac atagtctttt cttccggaac ctgtagggtc
     1021 aaatgctacc gactgtataa acgaaagctg ttgaatacta ctggacaagc tcaagtccgg
     1081 cagagagccg tccgatgcgg tgttgttatt tgatataaag cttagcaggc tttccgccgt
     1141 tcccacatcg gcaatttttc cgttgaccga tgatgcggtg gtttgcaaat aaaaaagctc
     1201 gttttgctcc accatcgcaa acgggcggtc aacatcgtat ccgtaggggt tggtcgtctt
     1261 gtcgtacccc gcggggacgg tgtttttcat cagtgaattt gccgcatcag atgaaaaggt
     1321 cggac
//
LOCUS       DAWAOG010000551         1318 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_38626_length_1318_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000551 DAWAOG010000000
VERSION     DAWAOG010000551.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_38626_length_1318_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          380 a          306 c          279 g          353 t
ORIGIN      
        1 cttatatgct gtttgtcagc ggctctgtcg cactttgccg acttctgatg aaaaacaaga
       61 ggtactacta caaagcaaat cactttgtat ccgtttccgg aatgacatac cgtatgaaaa
      121 gaaacggcgc aggactggct tccgtctgca tactgtcaac tatggtgctt gtaatgcttt
      181 cgtccacgat aagtcttttt tcgggaatac agacaagcac gtttatccgc agtcgtgaca
      241 tctcggtaac gctcacttac aacagctttg aagataaggc gatacgcaat atttccgacc
      301 tgtgcaatgt agcgcttgac gcggcaaaaa gcaatgatta taaccccata aactctcttt
      361 cgtatgatta tcttgaagtc ggcggttatt tttccgataa tgctatacac ttagctcctg
      421 cgaacgatca ttcagacgta agccaatctg aaataaagga tttttacttc atcacgcttg
      481 aagactacaa caaaacgagc ggcaggaacg aaacgctctc cgaggacgaa atactgacgg
      541 caagctcaga cggcaccgac agcgaaataa tattaaacgg caagaaatat aagtcaaggg
      601 cagttcctat gccgaaaaat atttcctacg gcgaaacggt ttacagcgta tttttcgtta
      661 ttctccccga taataacacc ctttatgata tattcctgct gaaccgtcag gcttacggcg
      721 aaaacgcatc gtggttaaag cattatttcg gctttgacgt tgacggaagc aacgccgaca
      781 agcaagccct gtgcgacagc ctcacggaca ttctggcaaa aagctcacgg aatatggaga
      841 atatcttccc ctttgccgaa aacagaaccg aaaaccttga tgtgatgata gcgacctacg
      901 gcggacttct ctttctcgga atattcctag gacttgtctt tgtctttgcc accgtgctta
      961 taatctacta taagcaggtt tcggagggct atgaggacag agaacgcttt gcaacaatgc
     1021 gaagcgtcgg aatgacggaa caggaaataa agaagagcat aaactcgcag gtgctgaccg
     1081 tgttcttcgc tcctcttatc tttgcaggca tacaccttac attcgccttt ccgataatac
     1141 ttaaagcagt taaaatgttc ggctttgccg acagcacact tcttcttatc gcaaacgtga
     1201 tatgctttgc cgtattcgcc ctgttctaca tctttgctta caagataaca tcggggattt
     1261 atcttaagat tattgaaagg aaataggctg tattgtggtt gtgacacaac ccggaaaa
//
LOCUS       DAWAOG010000552         1313 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_2752_length_1313_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000552 DAWAOG010000000
VERSION     DAWAOG010000552.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2752_length_1313_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          356 a          331 c          274 g          352 t
ORIGIN      
        1 ctctattact gtcatatcgg ctgtcttgcg tattccgacc acgccgaaca gcattgaaaa
       61 tacaaaaatg ccaaacactc ccgctatgca taaaagcgca gtctgtccgg cacttatttc
      121 tgccgaaaac gagccgtaga tcacaaaaga cagcgaaaat accgcaatac cgccgagtat
      181 tgacgacagc agtgacagga tcgcggtctt tgtcagaaag ataccataga tagcccgccc
      241 ggaaaatccg atgattttaa gtctgccgat ttgctttgaa cttcctctta cggatacagc
      301 cgtaaccgaa aacaggataa tgacagtaaa tacgaacata atcacacaaa gaacagcaac
      361 actgctaatg ttgcttattc ccgatatcac cgacagatcc gcactgctct tattcggaca
      421 gaatgctccc gcagtgccgt tttcgagcag cttttccgag tagcccgata tatccgtacc
      481 gtccgcaaag cttacgctta caaaacagta agcggtgttt tcactgaagg tgttatgcac
      541 aagtgcgttt atcataggcg gcaaaccgtc ccattgaagg cttgaaatat tgttcaatat
      601 gccgcataag gtaagctcgg tatattcttc attcagataa aagcctattt tgtccccgag
      661 ctttgcatcg ggataaacaa gatttaaaaa tgaacgctcg atcgcgatct cacttttatt
      721 atcagggaaa ttgccctcct gtaaggctat tcggttcagc aaaaccgcat tgctgtcggc
      781 ataacctaca gttatctgtc tgcccgagta ttcctcgctt ttaatgccgc ttcctatgac
      841 tgtgataaca ccgctgtcgg tgatctcgga aaggccttta agcttatcag gattttgtac
      901 cgcgaaaatg ccgtcttgca ctccgtaggt gttttctatc cttattcggt ttccgtcgct
      961 ccagcccagt aaaaacacgg actgcacgat aaaaaagagc gcggcaaaaa aatagagcaa
     1021 tgccgccgct atactgcttt ttttgttaca gatcagattg cgggtcacat aatgaaaaat
     1081 atatctcata tcactcaacg acctttccgt cgcatagtcg tatgatcctg tcatcttttt
     1141 ccgcaaactg tgtgtcgtgg gtgactatta tcaccgtttt gccaagctcg ctgtttattt
     1201 gtcttaacag gcgctgtact gccgcagaag atgccgaatc gaggtttcct gtcggctcgt
     1261 cgcaaaggat agctgacgga tcagtgatga gtgcacgcgc tatcgccact ctc
//
LOCUS       DAWAOG010000553         1237 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_10031_length_1237_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000553 DAWAOG010000000
VERSION     DAWAOG010000553.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_10031_length_1237_cov_5.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          350 a          263 c          309 g          315 t
ORIGIN      
        1 cacgttgagc tgtcaacaca gcccgaggac agtatgggca gccaggaaga ctgggatatg
       61 gctacagagg gtctgcgtaa ggctcttgac ggacttggac ttccttacgt tgtaaacgag
      121 ggcgacggtg cattctatgg tcctaagatt gacttccatc ttgaagactc gctcggcaga
      181 acgtggcagt gcggtactat ccagcttgac ttccagctgc ctatgagatt tgaacttgaa
      241 tacacaggtg cggacggcga aaagcacaga cctattatga ttcaccgtgt tgtattcggc
      301 tctattgaaa gatttatcgg tatcctgacc gagcattttg caggtgcgtt cccgatatgg
      361 cttgctcccg tacaggttga gcttatgcct attgcagacc gtcacaacga gtttacatca
      421 aaggttgccg cagagcttaa gaagcgtggc ataagagtag aggttgacga cagaagcgag
      481 aagataggct tcaagattcg tgaagcacag cttcagaaga ttccttatat gctggttata
      541 ggcgataagg aggttgaaac agaaaacgtt tctatccgtt gccgcaagcg tggcgatatc
      601 ggtacaatgt cggtatccga cttctgcgat atggtagaaa aagagataag agaaaagact
      661 atcattaccg attgatagct taaaggaggt cttgttatgg atcttacttc ctacattgcg
      721 agtatgtctg tcagtatggc acaggcaaac gtagcacaga gcgtttccac agcaatgctg
      781 tcaaagagca tcgacagcat cgagcaaagc ggggcagagg tagttgatct tatcaacagt
      841 gccgatattc cgcctgcagg agtaacagga cacgttctga atatcaaagc ataatttctg
      901 aaaactgccg atttttcggc agtttttctt ttcccctctt gacatggagt taactccatg
      961 tgataatata gataaaccga aaggacgtga tatattatga ctatttcaga agtcagcaaa
     1021 aagtacgata tttcaaacga cacgctccgc tactatgaac gtatcggttt actgccccct
     1081 gttccgagaa caaagagcgg tctgcgtgac tatgacgaaa attcctgcaa atccgtagag
     1141 tttatcaagt gtatgagaaa cgcgggactt cccattgaag cgcttatcga atatatgacg
     1201 cttttcaaag agggcgattc taccgcagaa gcccgta
//
LOCUS       DAWAOG010000554         1236 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_6551_length_1236_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000554 DAWAOG010000000
VERSION     DAWAOG010000554.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_6551_length_1236_cov_5.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          385 a          277 c          263 g          311 t
ORIGIN      
        1 ccctcttttt cactataatt ataaatgttt cagcacatat aattccaccc tgaaaagagg
       61 acaccaaaat gaaaaccacc ctatctgatg acataaacga aataaaaggc gagataacaa
      121 agacggtatc ccacggacgt gtgtacaact tcttccgaaa attgttcggt atccgtgacg
      181 atatgatgga caacgccgag atacagcaga tgatggagga caacaccgtt atcagcggct
      241 cgaatatgtg gatactgata atggcgatat tcattgcgtc tatcggactt aatgtaaact
      301 ccaccgccgt aattatcggc gctatgctta tctctccgtt gatgagcggt attctcacga
      361 tgggctattc gcttgcaacc tttgacctga ctttgcttcg gaaagcattt acacgcttcg
      421 gcattcaggt gataatcagc cttatcgcat caactttata ctttgccatt tcaccgctta
      481 atgtggctac aagcgaaatg atagcaagga caagcccgac gctgtgggac gtgcttatcg
      541 cattgtttgg cggtatcgca ggcggaatag gtaataccag aaagaaaaag agcaacgtaa
      601 ttcccggtgt ggctatagct accgcactta tgccgccgct ttgcaccaca ggctacggac
      661 tggctaccgg acagctgagg ttcatagcag gcgcatttta tctgtttgcg ataaatgcac
      721 tgtttataat gctgtcggca gcttttataa caaaactcat gcgtatcccg acccacaaga
      781 taaatgatac taaaaaggag cgcaaaatac acagatttgt agtgctgata acaattctta
      841 cgatcgttcc gagcgttctg atcggatctt ataaagtata cgaaaccgta atgaaacaga
      901 atcttttaag ctaccttgat aattcattta cattcagtga tacaaaagtc gtacaaagtc
      961 agatcgatat catgaacagt gaggtgtcat tatcgcttgt aggtacccag ctttctgatg
     1021 atgtgatagc ggtacttgaa aaagacctca gcaactacgg ccttgaaaac tacaagctca
     1081 atatcacaca gaacaaggcc gttaccgccg acaattccga tcagataacg atcgctctgc
     1141 aggagcagac tatcgaatcg cttcagaaac agcttgacga gcaggaaaag aatatgaagg
     1201 aacagcagaa ggaacttgac gaaatgaaat ctaccg
//
LOCUS       DAWAOG010000555         1207 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_42880_length_1207_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000555 DAWAOG010000000
VERSION     DAWAOG010000555.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_42880_length_1207_cov_5.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          263 a          296 c          266 g          382 t
ORIGIN      
        1 ccttatgata agtttcctgg aacaggatat atgtgcctat ccccgcttct ttcagctttc
       61 tgtaattttc taccgtagtt gcggcgatgt ttacattaac tctgcgaata gcgccgttct
      121 tgtgcttgat cgagtaaata gttttgatac attcgagtat gtattcaaga gggttgttta
      181 cagggtcttc acccgcttca attgcaagtc ttttgtgacc catatcctgt aaggcgatga
      241 cctcctgtct tacctcgtcc tgcgtcagct tcttacggca gatatgcttg ttctttgcgt
      301 ggtaagggca gtaaaggcaa ccgttcacac agtagtttga cagatacagc ggagcgaaca
      361 taactattct gttgccgtaa aagtcctttt ttatctgttc ggcgagcttg tatatctcct
      421 cgttcttttc aggaatgtcg cagtccagca gaacctcggc ttctctgtgc gaaagaccct
      481 ttctgagcct tgccttttca agaatgctgt cgataagctc cgcattatgc ttgttctctt
      541 cggcatatct cagtgtttct tcaatttcct cgtgattgat aaattcctca gccttgagtg
      601 attttgggtc gtaattatac atattttatt tcctttcatt ataagcggcg tgatcgcccc
      661 ttgattcgac tatttcatag cctgcgctct caacacgtct ttgcaggcac attctgcact
      721 gtgcggattc gtcacctgtg cagatcttat tgtcatacag cagatatttc tttcttacat
      781 ctgtcggcga cagattaggc attacaacgt ttgctcccgc tttaagcgca agctctcttc
      841 cgttcgggtg tatcgtaccc agtgctgttg ttgcgggaag aagcacatcc tgtaacacaa
      901 gtctgacaag cccaagcatg aacagcgtaa gctcagcagt accctgcggc atagcggcaa
      961 acggtgttgc gtggtgcggt ataaaaggac ctatccccac catttcgggc ttaagctctt
     1021 ccagaaacag catatcatcg gctaaatttt cagccgtctg atacggtgag cctaccataa
     1081 agcccgcacc cgtctgaaag cctatttttt taagtattcg caggcagtct tgtctgtgtt
     1141 ttgccgaaag ctccggcggg tggagctttg aataatgctc gcaatcggca gtttcatggc
     1201 gcagcag
//
LOCUS       DAWAOG010000556         1182 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_9554_length_1182_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000556 DAWAOG010000000
VERSION     DAWAOG010000556.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_9554_length_1182_cov_3.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          373 a          275 c          221 g          313 t
ORIGIN      
        1 atacctgatt gaacaaattt tcggatactg cagaagatgc caccaggagc tgtgaatctg
       61 ccgttgacat agtagaagca agtatacccg ctataataag accaccgacg atcgccgcaa
      121 gaggtcctgc cttagcaaga agatttgcta ttacaactat tatagtttct gggtctgcaa
      181 gtggatcgat aacattattc ttagtcatag caagacctac aacaccgatg aatattgcaa
      241 ccgcaagcga aattactacc catacagtag caacgcgtct tgaaatctta agcttattgc
      301 tgtcatttat agccataaat ctcagcagaa catggggcat accgaaatat cccagacccc
      361 acgcaagggt agaaacaata gtaagcggtc cgtaatcacc cgtctgctgt ttgagaacat
      421 caaatgtatg tgtcagagag aaataacccg gcatagaatt tgcgtgattc attacctcaa
      481 caacgccacc aacggaagaa atgccgaaca caacaactat aataagagca accgacataa
      541 tgatgctctg tataaagtca gtagtacttg cggctaaaaa tccgcctatg gtagtatatg
      601 cgatgattac gactgcactt attatcatag caatgtgata atctgctccg aaaaggctgt
      661 tgaacagctt tccgcaggct gagaagcccg aacccgtata aggtatgaag aacactatta
      721 ttataagcgc agaaataaga gtaagtatct ttttattatc cctgaatctg tttgagaaga
      781 aatcgggtat ggttatcgaa ttgcacacat gagaatactt tcttattctt tttgacacga
      841 taagccagtt aagatatgtg cctatcgcaa gtccaatacc tgtccaagca gcatcggcaa
      901 tacctgtcgc ataagcaaca ccgggcagac ccatcaaaag ccagccgctc atatccgaag
      961 cctccgcact catcgccgta acaaacggac cgagctttct tcctccgaga taaaaatcgc
     1021 ctacattatc cgtcttcttt gcgcatatca cgccgacaac taccatcata ataagataaa
     1081 cggcaattgt tatcagcata attatctgtt gtaattccat tttttgtttc ccttcgtctt
     1141 tttttgcggt aaaccgcgca ttacttttta ttgtatcata tc
//
LOCUS       DAWAOG010000557         1150 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_11744_length_1150_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000557 DAWAOG010000000
VERSION     DAWAOG010000557.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_11744_length_1150_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          252 a          294 c          259 g          345 t
ORIGIN      
        1 gaacgatccg ccgccgttta tattatcttt cagagatttc ggacatggtt atgtccgaat
       61 tacagctccg agccataatt ctttataaag tcgtgataga aaactattcc tcgttcaagt
      121 gactctacat taagattttc atttatagcg tgtgcgctgt taagctgtga acccgtcatc
      181 ataagcggaa caaatctcag tacgcagtcg catattttcg tatagtggcg cgagtcggtg
      241 cctgcgagca ttatataagg tattctcggg atatcgccga aggtttcctt tatcctcttg
      301 ttgacatatt tgtacgcata gcagttcata tccgcaacag gcggaacgtc aaagcctgcc
      361 agcatttcca tagaaatgcc gagcttgtgg cacagcttgc cgagcttttt atacgactga
      421 ggtaaaggct cgtgcaccat aaagcgacag tttgcggtaa cgcttgcttt ttcgggaatc
      481 acgtttgcac ccttgcttcc ctctgccata gtgaaaatgc aggtggtctt taccaccgct
      541 cccgcaggtc cgcctatcct gtgcattatg tacggtgcta tcggtgcaaa aagccataga
      601 tttccgtaca gcaggcggtg tctgaagtgg tgcatatacg gcgccatagc gtgatactgc
      661 actcttgcgg ggtgggttat ccttttcttg aacgggtttc tgtgttcgat tatgtacatc
      721 agctttgaca gctttgcaaa gggattattg ttgaacggaa cgcttgcgtg accgcccctg
      781 ctccttgcag tgaactttat gttggctctg cccttttcga gtatgcctat catagcgttg
      841 tgggctacca ttctgccctt gatgctgtcc tcatacgaca tcaccgagcc gccctcatcc
      901 ataacgagat cgaaatgtat gcccttttca taaagatatt cgaccgtttt tactgcgccg
      961 tcaccggtta tttcttcgtt gttggagctt ccgacataga catcgcacac gggcgtgaag
     1021 ccctctttta taagctcctc tatgctttcg agtatggcgc atagtgcgcc cttagtatcg
     1081 accgtacctc tgccccagat cttaccctcg gctatcttac ccgaaaaagc gtcgtacttc
     1141 cacttttcac
//
LOCUS       DAWAOG010000558         1148 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_23115_length_1148_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000558 DAWAOG010000000
VERSION     DAWAOG010000558.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_23115_length_1148_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          349 a          284 c          268 g          247 t
ORIGIN      
        1 cccaggcaca gctcagagcc gatattatcg aaaaggtcat aaaggctacc gttcccgctt
       61 ctctgcttga cgaaaacaca aagatgctga taaatcccac aggacgcttt gtagtaggcg
      121 gtcctcaggg cgacagcggt cttaccggca gaaagataat agtagatacc tacggcggtg
      181 tcggcagaca cggcggcggc gcattctcgg gaaaagatcc cacaaaggtc gaccgttcag
      241 ccgcttatgc cgcaagacac gttgcaaaga acattgtagc cgcaggtata gcaaagcgtt
      301 gcgaggtcga gcttgcatac gctatcggcg ttgcacagcc cgtatcgata tttgtcgaca
      361 cattcggcac aggcataaag cccgataccg agatctcagc cgcagtaagc aaggtgttcg
      421 accttcgccc cagcgcaata atcagctccc tgaacttaag aaacaccaag tacagacccc
      481 ttgccgctta cggtcatatg ggacgtacag accttgatgt tgcatgggaa aagacagaca
      541 aggtcggcga gcttagatca gcacttgata taaagtaatc agcatatagc actaaagccg
      601 ttacgcaaca ccgcaacggc tttaatcgta accaaacgga gaacagaaat gacccatatc
      661 cgccacgcga ccgagtctga tgcttcccgc atcgcagaaa ttatcgtcac aaattaccga
      721 gtaaatttct atccgttttt caaaaacgat gccttttact tcggtgagct gaatgtcctt
      781 gacactgcca aggagtactc agcgggctca accgcactca aaaatacatt tgtctatgat
      841 gacggtataa taaaaggcat tatccgcatt tcgggcaatg aggtcgaaaa gttgtatgta
      901 gagccgcttt ttcaaaacca aggcataggc gcaaagcttc tcagatacgc tgtcaacaac
      961 ttcgcggtca atgagctgtg ggtgctcgaa tataacaagc agggaataga tttttatcac
     1021 cgcaacggat ttagccttac aggcgaaaaa atcatagaag acgaatttgt accgctttta
     1081 aagatgaaaa gagaataagc cgaaagaagc ggtgacgaaa tgccaaaatt tcatcacagc
     1141 ttctcggc
//
LOCUS       DAWAOG010000559         1122 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_42650_length_1122_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000559 DAWAOG010000000
VERSION     DAWAOG010000559.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_42650_length_1122_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          394 a          192 c          267 g          269 t
ORIGIN      
        1 gttgcaacgg tcaacggtac tgatatcacc gaggaacagc tacaggagca attaaagaca
       61 aatgcggttt tcaaagaggt tataagcagt catattgacg atatagctac acctgactat
      121 aaggagatca cgctcaaaag gcttgacgag cagtgccctg ctgacaggga caaggctata
      181 aaaaaactgg ttgaaacggc ttatttcctc ggtatggata acagcatatc aaaagatgaa
      241 gccgagaaac agataaagca gcagcttgac gatcttgatg catattcagg tcaatatggc
      301 aatgtaaaag tcaaccgtga gataatgaac gaatgctttg aaaaactgtc gaccacggaa
      361 gagcagtaca taaaagacag cgccgattcg tatatcgcaa tggtgaacag gcagagaatg
      421 tacaacaaat ttattgaaga tgaaaacctt gttgtcgatg aaaataacag tgataaggtc
      481 agaaatgaat ttgagcaata tcttgacgaa cagctgaaga aagccgatat tgtgtattac
      541 aacagttgat agaagtaacg gaaagtttat gtaaaaccgg atatcaccta tatcatatcg
      601 ctttaggcag agggaaaact atggatataa agacgcagaa ctattttaaa aactggagtg
      661 atacggcaag ttggtcgacg gacggctcac tgcaatacga tgaacgaaac aacgtgttgt
      721 attttgataa gacaaaggtt acggaaaagt tgtccgatgt aaatgaagac tatgatgttg
      781 cggcagtgcg gacatttgat atacctggcg gcagtcacgg gttttatttc tcggtcacgc
      841 tcggaaacag ctcaccgctt ttcgataaca acggcggacg tatagacatt gaattttatg
      901 acgcaaacgg tgtaaagaca gtaggagatt attcaacttt gtacatatat aatgcagaga
      961 atttcatcga ggcatatctg ggcgatgaca agcctgcacc tatcccggaa gatgcaaaaa
     1021 gtatggttat aaaggcatac ggggtttccg atacaaaaac gatagaattc tatatgaaag
     1081 actttaaact gtcgttttcg gacagtgagg actgtatgga aa
//
LOCUS       DAWAOG010000560         1100 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_20562_length_1100_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000560 DAWAOG010000000
VERSION     DAWAOG010000560.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_20562_length_1100_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          337 a          213 c          277 g          273 t
ORIGIN      
        1 cctgccttgt aataagcatt gttctgtatc ttctggcgga attgacgcac ggcagaaaaa
       61 tacgcaagct gtggcagtcg tttgtaagat acggctgtgc ggttgcaggc tcgttactgc
      121 tgtgctttgt tatgaggtcg tatgacattt ttggacttga acactatgta ccgtctacgt
      181 tttttgtaga caacgtaagc gtttcaacgt caaaggcttt caatgaattt tgcggcggcg
      241 gtatagtcaa caacggattt caggagtttg acgatgaaag atcgatcaaa gcgataaccg
      301 attatcattc attcatagtc aataacggtt atgcgcgtgc aggcggcaac ggggtcgttc
      361 attacatcag tacgtcggac gatcaatata ccgactatac tactgtgagc tttaaatatc
      421 gcctcaaaga cggaagcatt gtgacgagaa catttaatgt caatatccaa aaggataaga
      481 gcgcagaata tgcgaaaaag ctgtacgata tgagatgcgc catgtccgaa agtgacggat
      541 atcagtcatt ttttgagtat ttcggaagcg caggaaacgg ttgggatatt attttccacc
      601 gcgatgatgg tgagctgtac ggaagaaagc ttaaaacgga gcttaacgaa aagctgatta
      661 aggcgctgaa aaatgatatg aaagcgggca gaaacacagg aaagctcgct gctgtgctcg
      721 actttgtcct cgctgatgca acttattcct caatgagcgt tgagatcaga gagaactgca
      781 aggaatcgct tgccatactg gaaaacaagg ataattcgac atcgcgcagg gaaaaaataa
      841 tcggtgcttt aaggactgct aagtacgaat atgacaatac ggaagcaggc acagagtccg
      901 gcagcgacga atacaacaca agcgtaggag tggaaaccga ctacaatata tataaaattg
      961 aaaaaatact ctcgccatac caggtttctt acagctcgga ttgggtatat gccgtcaatg
     1021 cagagatggc cgctgttcca tacggtcttg cagacagcga agcggtgacc gaatttgaaa
     1081 agctgtgcaa aatgtatgac
//
LOCUS       DAWAOG010000561         1096 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_27348_length_1096_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000561 DAWAOG010000000
VERSION     DAWAOG010000561.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_27348_length_1096_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          296 a          242 c          247 g          311 t
ORIGIN      
        1 tcaagcgaag ccaggagctc actgtcgctg tttccgctgt ccgcaatgct gtagcttatc
       61 cgcttctgct tataaagcgc gtggagcgga cggataacgg aattgtgctc aaggtctgat
      121 gttatgacgt ggcaatgtcc cacgctctga catacgccct taacagcaaa atttatcgcg
      181 tgtgtacagt taagcgtaaa tacggtgttt tcggtttcgc cgccgaaaaa ttccgcgcac
      241 ttttcccttg ctccgaatac ctgctttgag gtttcaagag aaagcctgtg acccgatctg
      301 ccgggatttg ctccgtaatc gtacagtgct ttttccgctg tatggagcac cgaaacaggc
      361 ttcggatagg tggtggcggc attgtcaaga tatatcatcc cgcatcaccg ccgtttctga
      421 tctcaaggca gttaagtccg ccgagggaca gcagtcggca ggtcttgtcg gggtcgtatt
      481 ccgtataaat tccgaaacgg cagcctttgg tcgtcgttat ttttcctgta gaagatgata
      541 tcttgtaacc gctgagaata cgtcttgctt tctcggcggc agtagccgaa cgcagtacta
      601 taaaatacat gatgtgcacc tcgtttccat atattaactg acgtattata tgccaaacaa
      661 cgcaggaaag ttacgttttt gcggaaagat tgcaatatac cgcgctgccg atgccgactt
      721 gccacagctt tggcgtaaag cttaaaaatc aaggcttatc aattggctgt ttgaaattca
      781 gttacctaaa tttgcttaaa ttgcaactta aatttaccgg tttttgatta aatatacaca
      841 tataatcttc actgcgtttg tgcacattaa ccaaaactgt tgataataaa ctgtagaaat
      901 agtgacttga aattatgaac ggtttcggtt ataatataga tatcttggtg taaacgaata
      961 catacaagta cgattaaaag gaggactaaa atgaaactct caaagagaat actcgcttgt
     1021 gcggttacag cggctatggc aacatctatg ctttcagcct gcaacggttc aagcacaaca
     1081 agcagttcgg ctgaca
//
LOCUS       DAWAOG010000562         1088 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_31064_length_1088_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000562 DAWAOG010000000
VERSION     DAWAOG010000562.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_31064_length_1088_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          265 a          254 c          224 g          345 t
ORIGIN      
        1 gataacaaag ttcatttcag ctttcgcacc tactgtcgta tcctgcataa gtatgcagtt
       61 cttgaccttt gcgccctttt caatctttac gcctctgaac agaacacagt tctcaaccgt
      121 accctcaata acgcagccgt cggcaacaag agaattcttg acatctgcgt caatgccgta
      181 ctttgcagga gcgtcatctc ttacctttgt gtagatagga gcatcactca taaacaggtg
      241 atcgcgtaaa ttcttgtcta cgagcttcat gttagtcttg aagtatgtgt ccatgctgtc
      301 gatctgggcg aagggctttt caaacttgta acccagaacc tttatctcgt gtaatttttt
      361 ctgaagtacg tcaatctcaa agctgtagag attgcgactt tcggcatcac gaatctcatt
      421 taaaaggaag tctttgctta caacgaacat attgagactg acattgtgtt cgccgctgat
      481 ttcaggacgg ataagaacat cgtatacaac gttgtctgcg ttgactgcca gaacagtctt
      541 tgtcatcgtc tgttctgttg tgaatgttcc tctgccgtaa actacagtga tatcagcgcc
      601 gtttttctca tggaaatcaa ttataggtct gtagtcgata tttgctacta catcggtatc
      661 cgaaagcagt acatattcgg catttgagct ttctataaag ggttttacct gagccatagc
      721 ctcaagtctg cctctgtaaa gggcattgct gttgccgtaa gggggaagca ggtgcattcc
      781 gcctgttttt cttgaaagat cccattcatc gcctgagcct aagtgatcca taagcgataa
      841 gtagttatct tttgtgatta taccaacatt gtcgatatcg ctgtttacca tatttgacag
      901 cgggaaatct atcatcctgt atctgccgcc gaaaggcacg cttgcaagtg tgcgctgttt
      961 tgtaagatcg gctacgctct gctcgtgcat atttgcaaaa atcagaccga gtacatttgc
     1021 catattagcc atttctcttt tctcctcccg ttcacacgtt agccgagatc attgcctttg
     1081 gcttgatc
//
LOCUS       DAWAOG010000563         1082 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_32611_length_1082_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000563 DAWAOG010000000
VERSION     DAWAOG010000563.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_32611_length_1082_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          252 a          268 c          249 g          313 t
ORIGIN      
        1 tactttacgc cgaactcctt cagtgagccg ccgtgttcgc cgtttatgac ctctgttaat
       61 gtatcatacg gagcgcctgt tacgcagagc agtgtatcgt tcggacggag tatgccgaac
      121 agggcagtgg agagagtgtg cgtaccgttt acaaagctgt ggcggacaag tgcgtcctcg
      181 gtatggaaaa cctcggcata taccatgtcg agcttatctc tgccatcatc gttatagccg
      241 tatccgatag tgcctttaag gtgcatttcg ctgacacggt tgtttatgaa tgccgagagt
      301 accttgtttc cgttgtactc tgcggtttcg ctgatttttg caaactgcgc cgaggcaagc
      361 tcttcggctt tctttgcggt ttcaagcagt ctgctgtcaa tattgaaaaa ttccattttt
      421 atcctcttgt tatttcatac gctgtgcctg tcgccgtaaa accgcagttt atatagaact
      481 gccggacgtt ttttttgcag gcaagcgtta tttttctgtt gtcaagtaac cgggcggcac
      541 tgctaagaag cgcctttccg acaccgtttt tctgcatatc ctgttttact gcgatgtggg
      601 acagaaagca acttttatca ttgccgtaaa gcactgtcgc agtcgcaacg ggaatatcac
      661 cgctgtacat tactataagt cgcgaaacgt cgtgacgtac acggtgacag gtgtcggcgt
      721 accagtcatc aaactgatta tcgctgagcg caaatccgct tttcagtatc tcaaataccg
      781 tgcggtagtc ctcggtaaac tccgctttca gcagatcgtt acacacggca ggcattttgc
      841 ccgatgtcat tatataaagc gtttcggatt tttccgaaag tctgatatcc gacattacag
      901 ccctcgcgcc gagcatatcg gaaaacgcaa gcaggctttc tgtatctgaa ttttcattac
      961 cgtgaacata aagctcaccg ccgtaataaa gcgcaagagt attattgccc tcatagaatg
     1021 tgcagaagtc gtaccctgtg ccgtaagccc tgtaatacgc atttatccgc ccgactatca
     1081 ca
//
LOCUS       DAWAOG010000564         1082 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_3944_length_1082_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000564 DAWAOG010000000
VERSION     DAWAOG010000564.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_3944_length_1082_cov_3.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          347 a          234 c          271 g          230 t
ORIGIN      
        1 atcagctttt ccgtaaaaaa gagcgaggtc atagtaatag taggaccgag cggctgcgga
       61 aagtcaactc tcctgcgctg tataaacgca cttgagccga tacaggaagg cagtataacg
      121 cttgacggcg atgtaatcga aagaaacagc aagacgcttc ctcttttaag acagcgtata
      181 ggaatggtgt tccagagcta tgacctgttc ccccacctga cggtgcttga caatattctt
      241 ctcgcacctt gcaaggtaca gaaaagggat aaagaagagg taaagcagga agcaatgtca
      301 ctgctcgaaa gagtgggact taaagaaaag gctaaaagct accccagaga gctttccggc
      361 ggtcagaagc agcgtgtggc tatcgtccgt gcgctttgta tgcaccctga aatactgctc
      421 tttgacgagg taactgcggc actcgatccc gaaatggtcc gtgaggtgct tgacgttatg
      481 ctcgatcttg caaagcaggg tcgcacaatg ctgatagtaa cacacgagat gcagtttgca
      541 agagcgatag cagacagaat aatcttcctc gacaacggaa agatcgtgga agaagcaacg
      601 cccgatgagt tctttgataa tcctaagaca gaaagggcaa aacaattcct taatacattt
      661 gagttcgact cggtaagagc aaagaaagaa ctcgcagaat aaagagagat atttaaagga
      721 gataatcaca atgaaaaagt taaacttcaa gaaactgata gcacttggcc ttacggcagc
      781 tgcggcatta gcttcattca cagcgtgcag cacaaacgca acaagcggcg gcagtgacgg
      841 cagcacaggc tcaaaggtat accgtacact cgacgagata aaggcaagcg gtaagataaa
      901 catcggcgta ttctcagaca agaacccctt cggctatgta gatgagaacg gcgaatatca
      961 gggctatgac atttattttg caaacagact cggcaaggat ctcggcgttg agataaacta
     1021 tgtttcaaca gaggccgcta accgtgttga atatcttgaa acaggcaagg tcgatattat
     1081 cc
//
LOCUS       DAWAOG010000565         1067 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_47045_length_1067_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000565 DAWAOG010000000
VERSION     DAWAOG010000565.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_47045_length_1067_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          264 a          272 c          204 g          327 t
ORIGIN      
        1 acccgtatcc gctcatataa tctatcatcc cgtactcgtc aataagattg gcaacagcag
       61 gtataaactc attttcccac ttgtatttgt caggatagag cgaccttgcc gccgccatat
      121 aggatttaag cgtattgtca ggctcaaagc caagcccatc cgggcttttc ggcgcattcg
      181 gaaacggatt ctcatataat cttgtataat gagcgcacac gttcctgaga tccgacaggc
      241 acagcattct gtcctctacc gttctgtagt tcaggtcaaa atgccgtggc accatatcct
      301 tcttatccgc cgttttcata tcgataagaa aatatgtcag cgttccgaag ctgaacagct
      361 ctacagccgc ccacaccggc attataccgc cgtattttct cttgtggtgt tttacaaatt
      421 cctcgttctc gttcgcctcg ataagccgct ctatcttcga caaaaacgcc tgatgattat
      481 gacgcggatc aaagctcgat gcgttgagat atcccagtgc cccgtacttc atagcgtgat
      541 agttggaaat tatcgcgcgc atcgatattt ctatctcttc aagatatgtc attatcatac
      601 ggcgcaatat gcggtcaaag tcgtacacct tcattatctg ctcaaaggtc gttccatctc
      661 tgtacttacc cttacgctcc agaaacggcg caaagtaata agcaagacga tagtaattga
      721 tattggtaag gcatttttcg gcgtactcct catctgctat tatacagcct ctctgccgca
      781 gtatatctat ctgttgtttt atggtcgcgg gggttttact gaacagcatc ggttattttt
      841 ccgcctttcc ttgtatctat gaaatgtccg agtgtaaacg aatatatcag catttgcttt
      901 accggcattg aaaattcctg ttcaagcgct tgtctgtata tatcaagctg aagcttgtaa
      961 tcatctatat acgtctgctc aacgtccgtt ctgtcgctct tgtagtctat aagcactatt
     1021 tctccgtcct caatgaagaa catatccgca ataccctgta catacgg
//
LOCUS       DAWAOG010000566         1058 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_12418_length_1058_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000566 DAWAOG010000000
VERSION     DAWAOG010000566.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_12418_length_1058_cov_6.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          225 a          286 c          230 g          317 t
ORIGIN      
        1 gaagaatgtc agtctttcgg gcatttcgtt cagcgcgtct accaccttgc gatccataag
       61 cttatagtcg cttgcgccgt ccagctcaat gttcgagctg ttcttcatca tacggtaaaa
      121 ggtctttgcg aagagcttgt atactatgcc ctccttgccc cttgacgctt ttatggcttc
      181 gactacctcc gcgccgttct tccacgcggt gtacatcttt tcaagaagct cgggcgggtg
      241 ctgtagatcg cagtctatca ccgcaacaca gtcgccgtct gcggctttaa gtcccgcaaa
      301 gatagcgccc tccttgccga aattcctcga aaaatgaagc cctcttatat gctcgtcagt
      361 ttcgccaagc tgttttataa gctcccatgt gccgtccctt gagccgtcat caacaaaaat
      421 cagctcatag tcatcaagcg gcgctaatat gccgccaagc ctttcggcgg cggcaggtat
      481 attctcctgc tcattatacg caggtattac tactgaaatc atcttattct ccgactatat
      541 ttatctctac tgtcttttca cgttctccgt caaagtccat atatattatg tattgcgaaa
      601 gtccgagtga tatctcgctg tcgcagatag ccgcagtcac cgagccgccc gcaagctgat
      661 ggcagatagc cgccctgagc ggctcttccg caagggtaga taccttttca tagtatttga
      721 ggtatccatt cagtgcctcg gggtcgtttg tcgcggtgaa cacagaggcg gtagtatgca
      781 tagacgaaac tgttatgata ccgtttctga tattggatct tcgcacgcac tccattacat
      841 cttccgtaag atccatatac cctgtctttg acgggatagt gatagtaaaa gtctgctttg
      901 cgttattcat aatacatatc ctttcatatt gcggctttgc cgcgttactg agcctgcttt
      961 acatttgtat ttattcttac tctgcccgag ggcgaggtta tcccgagatc gtctttttct
     1021 gcggctctgc cgtcactcat agcggcaagt ccgttgac
//
LOCUS       DAWAOG010000567         1053 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_47007_length_1053_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000567 DAWAOG010000000
VERSION     DAWAOG010000567.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_47007_length_1053_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          384 a          181 c          245 g          243 t
ORIGIN      
        1 aaccgacgat gagctgaata taacgatgaa accgcttact gacaaaatcg gaaggtcgct
       61 atcacccgca aaaaagacgg cggacgatac agtagtatac agtgatgtat ttgacagcag
      121 caccgatatc gaatatactt atacatacgg cggagtaaaa gagaatatca tattagaaag
      181 ctataacggt aaaaattcgt ttgactatga aataacgacc ggcggtctta cattgcatga
      241 ggacaagggt actttactgc ttgccgatga aagcggtgat gtaaaggcta cactcggtga
      301 ggtcatagta ttctcggctg acaacaagca taacacgatc ggaaagtata aggtagagga
      361 gcttgagaaa aacaacagat ataaggtaac actgactgtt gacaaggaat atcttaccga
      421 cccggataca aaatatcctg tcaagatcga cccatatata aaactaataa agataatctc
      481 agcatacagg atcttcaagt tttcaaaggt aaggacggta agggaacaag cgaaacatcg
      541 ctcggaactt ccggagtttc aagagtaggt tggactgact ggggtgcgtg caggactttg
      601 cttaaactga acggatttag tttcagttca aaaggtatag taaatgcatc gcaaattcaa
      661 agcgcatatg ttgagatacg tgatctgatg tgtcaaagca cgaaaacgca aatcgtatgt
      721 gcaatgttta aaggcaataa ctggactgaa aaagatgtaa aaacatggaa tgcgttaaac
      781 gcaggaagtg cggcaggtcc tataaagatt tatcagaagg acaaatcaaa acctgcggta
      841 tatcagcata ccgtaaaggg cgataacggg cagagtgtac gcgacggtta cagtaaacaa
      901 tggtatgcgt gggatataac aactatagct aaaaactggt ttaaaaacag ttcttctatg
      961 gcaaaaggtc tggttttcaa aactgcaagt gaaacatatg aaataagctc aaagtatgca
     1021 aagaatatga aaactttcag ttctatgcag ggt
//
LOCUS       DAWAOG010000568         1047 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_21225_length_1047_cov_3.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000568 DAWAOG010000000
VERSION     DAWAOG010000568.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_21225_length_1047_cov_3.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          243 a          243 c          213 g          348 t
ORIGIN      
        1 caggacaaaa gcgcctgttt gtcttcaact tttaagtata cgttgaaaac gataaggtcg
       61 ataagtcttg aaagatcttt ttcgctgagt atgccgcgat aaagctcgat aggcattctg
      121 ggtgacattt cgctgtactg cctgaattcg ttgttgatag cgcgcacaaa cgctgtcttt
      181 tcagcggcac tgagtgtatc gctgagttta ggcatagggg cgatatccga aataagataa
      241 gggtcttcct gtgttaaggc tttaagcttt gccgtataaa gcccttgtac aagtacgcgt
      301 cttgcgccgt caggagtatt aagcgtctgt cttatctcgg caacgacacc tgtcttatag
      361 agattgtctg ctgtaacgtc atcaacctct tcgtctatct gtgttacaag gaagatacgt
      421 ctttcgttgt tttctatagc ttcttcaacg gcatttatag agcgttctct tgctacgtca
      481 aagtgaagta tcatacccgg aaatattaca agtccacgca gtgcgagtgc cggcattgtt
      541 atgtattcgt ttttcatagt catctgtcct ttcctgtcgc tctgtataag tcaaaacatt
      601 tctgttggtc tacccatatt atgctgtttt gtattgcaaa tgtcaataca ttattatact
      661 atattcgtcg cgaatttgca agtagtagcc gctgtaaata aaaaccgccg tacatctctg
      721 cacggcggtc atactttata tccttatgtc gaaaacaaat tcggattact tgctgaactt
      781 agcgtctgca atttcgtcca tagacttgcc tgcatcgtca ttccatgcat acagcttacg
      841 gatctccttg cctacctgtt caagctgctg ttcgctttcg atacgtctta ctgcgttgaa
      901 gtgtgaacga cccatcttgt tctccataat ccagtttcgt gcgtatgtac cgtcctggat
      961 ctcgccgaga accttcttca tttccttctt tgtttcgtct gtgataagtc tcttgccaac
     1021 ctcatagtca ccgaactcag ctgtatc
//
LOCUS       DAWAOG010000569         1040 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_7600_length_1040_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000569 DAWAOG010000000
VERSION     DAWAOG010000569.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_7600_length_1040_cov_5.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          271 a          285 c          215 g          269 t
ORIGIN      
        1 gacagtatat aacgccgctg aatacggcat atattccgtg ctgtacgggg aagaaatgag
       61 cgctcctctt atcccttgcg tattcagccg aataaagatt atcattcttc ctgccaaaac
      121 gggatatttc ctgttcctca cgggaaaacg cctttactac cctgtgtccg ttcagcgcgt
      181 ccgacaaggt agacgtaacg cctcgtcttg cgatatcacg gtttctccag tgccttcgct
      241 gacctctcag ccaaagcacc tcgcacagca gtacaaagaa caccgcaagg aacatcgaca
      301 gcgatattat cgggttcacc ataagcataa gcacgacaag tcctgctatc tttatcatat
      361 ttgcaataaa ctgcggaact atatccacaa aaaatccgta cacatcaaag ctgtcacggt
      421 ctaccctgcc cataagcgag cctgtctgct ttgttgtgaa gaatgacagc gacagcttct
      481 gcatagaagc aaaaatatcc gtcctcagtt tatgtattac ctgcggtgtt atcctgctta
      541 taataacacc gtagatcatc gaaaaccccg tagacagcag tgagaacgcc gccattacca
      601 gtatcacgaa cagtatctgt ccgtacaggc ttccgccctc ggtcagaaca tcatcgtaca
      661 gcatctttgt gccgaagtac ggagctataa gccccagcgc cacagatacg gctatgagcg
      721 ccagaattat aactatctgc tttttgtagt caccgaacat acccagtaag cgcttgaata
      781 tccatgtacg ctttgtgcaa tacggacaga atgctctgtt cgggtcggga tattttctgt
      841 tgcacttcgg acaatacagc gtcttgtcgt ccagtgccga gtcgtcgggc gtttcaccct
      901 ttttgatcgt ttcgtatctg cggcagaact gctcaaattt ttcggaaaac cctcccgaaa
      961 acattgcgag ctgtttttcg ctgccgtcac tcatctttgc catcagcctt gccgtaaact
     1021 gatagcggtc aacatacagc
//
LOCUS       DAWAOG010000570         1033 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_11988_length_1033_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000570 DAWAOG010000000
VERSION     DAWAOG010000570.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_11988_length_1033_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          242 a          256 c          214 g          321 t
ORIGIN      
        1 ctgtcgggat ctctctccgt tctgcttata gttatcggct ctgacgggcg gataataaac
       61 accttgccgc tttgttcaag ttcgcatagc ttgtctatgc tttcgttgta gacattgtga
      121 cggttttcaa gagcattgca caagtttgga aatttgccat agaatgagcg tatcagcagc
      181 ttgttcaacg gctttttgcg gtagctcata tcacgggtga ggattaccac ctgctttttg
      241 tagcccattt cagaaaaatg ctcaaacggt atgctgtcag aaattcctcc gtcaagatac
      301 gccctgccgc cgattattac aggctttgat atgaacggca tagaagcaga agcgcggagc
      361 atttccatat cccgaagtac gctttttacc tgcagatact ccggctttcc cgtttttaca
      421 tcggtgaccg ttgcataaaa aggcacaccg gaatttatat aggtttcatc gtcgaatttg
      481 tcaagctcgt tcggcacttt gtaataggca tattccgtgt tgaaaaagtc gccgctttta
      541 agtagcggct ttattcccat gtagttcctg tcgccgttga aacggcagtt gtagcggatg
      601 accctgccgt cctgccttga cagaaaattt actccgaaca ccgcacctgc cgatgttcct
      661 attatcccgt caaattttat atcattttca agaagtacgt caagtgcgcc tgccgtgtac
      721 attccgcgca tagcgccgcc ttcaagcaca agtcctgttt tcataatgtt ctccgttaaa
      781 tttatttgcc gtattcgttg tcgaactctt gtatttctct gagcatatcc tcagtactgc
      841 cgtcgcttga atcataccat ttcatctttt tcagatactt gccgagccag ctgtcgtgaa
      901 gagcatttgc gacaattccg acctcactga aagaatcgtt accgtccccg atcacaaaaa
      961 taagctgatt atcgtttgtt gacagctcat tgaagctaca gtctgcaaac gcttctctga
     1021 ctgtcttgta tac
//
LOCUS       DAWAOG010000571         1017 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_41922_length_1017_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000571 DAWAOG010000000
VERSION     DAWAOG010000571.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_41922_length_1017_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          324 a          237 c          185 g          271 t
ORIGIN      
        1 ctgtaagaat atcggaaaag ccggaagtat cggcaactac aagagcttta agataagtaa
       61 gatgcttaag ctttttgtct tgtatactca aaaaagcgtc acgggagatc atactgtcat
      121 atacaaaccc cataggtata taatccggat tttcgtatat cacatattct cccattgtcc
      181 cgactttttt gaatgtgtca tatttgtcac ctgttttgcc aagtcccgca aaatacttta
      241 ctgaaataag gtcgcagagt tccttgtaat caaaagctat cctgtcgtat acgccgcttt
      301 cataaccgag tgagctgtcg tcaaggaact gtgtaaatcc ttcgtcatgc aaactgttca
      361 gatgcgacgg cgaatccgca ccccacacga gattggcgtt tgtcgcagac agagctattc
      421 tgggactgcc ctccctgcac tcctccggca aatcctctcc gaaggcaaat gccgccgtat
      481 tggcatcttt actcggtatg cttgaataaa gatattgcac cataactcca tagcacatat
      541 atgtgcatat tatcgaaaag atatatagct tcggtataaa atccttatca cgctttgatt
      601 tcacaataac aatcaaaagc acaagcgaca gcacggtaac ggcaaaatga agcatagtct
      661 gataaacgct gaaattcgtc ctttgcgaca ctttacttat aaatccgtta gtatcaacaa
      721 ccagctgata tataatcatt gcaaaaacaa ctgccgtaca ggtaataatg ccgggcttga
      781 agcttatctt ttcctcaaga gcggataatg acatcacaca cgcaatcagc atcggcatat
      841 acagccatct tacataatat gcactgttca gagcggaaaa cagctggttt aatacaggca
      901 caaacgccat aactccgcat attaccataa taattgtcag ccaagatttt tttcttgccc
      961 acgcaaaaga aataacgcct gccattgaaa atagcggaat atagaacgca atagaag
//
LOCUS       DAWAOG010000572         1016 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_41014_length_1016_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000572 DAWAOG010000000
VERSION     DAWAOG010000572.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_41014_length_1016_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          349 a          191 c          226 g          250 t
ORIGIN      
        1 aaatgctttt ctattctgaa aattagctga aacagattga tttttacttt tcgttgtggt
       61 aaaataatgg atagtttatg taagaaaagg agcttataat gatcaaaaaa atattagcgg
      121 gcgttttatg tgccgcaaca atgataactt tatctgtcgg ttgttcaggc aacagtgcat
      181 caaccgatag tacgtctgtt gacccttctg caaagataac aggcaacata ggtgatgtaa
      241 agcttgaaaa gggcgataag tacgctgtaa tgacaataaa ggattacggc gatatcacta
      301 tcaagttata tcctgatgcc gcgccgaaag gtacacagaa ctttatcgac cttgcaaaca
      361 gcggatacta taacggaaag acattccaca gagtagtagc agactttatg gcacagggcg
      421 gtaaggattt tacaggtaag acggacgttg agaacttcgg catcgaaacc aactacaata
      481 tgcgacattt ctacggtgct ttatgctatg caaatgcaat gggcaaaaat tctacagagt
      541 tctacatcgt aaacaacaag aaatcgcagg attacagcag cttcagcacc tcaagaatag
      601 acaacaacat tcagggctat gaggactacg caaagcagta cgacaaggat tctcaggaat
      661 atacctacta tatgttccag gcaaactact acagaaactt aaagcagttc atagagaata
      721 tggacgacgc tacaaaggca aagtacaagg aagtcggcgg cactccctct cttgacggta
      781 actacaccgt gttcggtcag acggtagacg gctttgacgt tcttgacaag atttctgccg
      841 ttgaggttga aacaaacgac gctatgggtg gcaaagaagt aagtaagccc aagactgaaa
      901 tagttattga aaaaattgag atcaaggatt atgaataata tcagataccg ctccgcgtga
      961 gcggtatttt tttattgaaa acaaaggggc aattctgagg caataccgca caaaga
//
LOCUS       DAWAOG010000573         1008 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_9045_length_1008_cov_4.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000573 DAWAOG010000000
VERSION     DAWAOG010000573.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_9045_length_1008_cov_4.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          328 a          173 c          240 g          267 t
ORIGIN      
        1 gaataactgt agcattactt gcggcggttg tgacggtaag ttcgtttaca atcaatgcgt
       61 ttgcggacag cggctcggac aaaattgccg tttctactga aaacgatacc tattcggatg
      121 gaaatgaaga tattgaaatg ccgccggaaa cttcggacga gcgaatatat gttaagtgta
      181 catctttggt tgatttgcag agtgatcacc cttatgaaga tgattccgac atcacatacg
      241 tttacacaga taaaagttgt aataatctga agattacctt ttctgaggac acagaatttg
      301 aaaatcgcta tgattatctt acggtatatg acggaaacga taacgaaata ggaaaatatt
      361 caggtactga gcttgccggc ggctctgtta aagtaccggg taatacagca aaactcagac
      421 ttacatctga cggcgtaggc aattgttacg gttttaaggt tgattctgta gaaaatattg
      481 acaatgtgcc gaagcagggc gacccggaat atgattatat tctgtcagat aacggaacag
      541 acgatgatgg aaatcctatt tacagcatag aacaatacag gggagccgga ggaaatgttg
      601 ttataccttc agaaataaac ggattaaagg taacagcaat acgctatgga gcattcaagg
      661 aatgcacaac actgaagagt gtaacgatac ctgacagtgt gacgagtata ggcgctagtg
      721 ctttcagtta ttgcagaaat ctaacaagtg taacactgtc aaatggtgtg acaagtatag
      781 actggggcgc ttttaattgt accggtctta caagtataac aatacctgac agcatgacat
      841 ggattggtga aatggttttc ggtaattgca caaaccttaa aaatgtaact ataccgaaca
      901 gtgtgacaag tataggtagt tcggcgttta gcgattgtac aagcctgata agtataacgc
      961 taccggacag cgtgaaagag atatgcagtt cagcattcag cggttgta
//
LOCUS       DAWAOG010000574         1002 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminiclostridium sp. isolate gSpreadC_ID_896
            k99_47745_length_1002_cov_5.0000, whole genome shotgun sequence.
ACCESSION   DAWAOG010000574 DAWAOG010000000
VERSION     DAWAOG010000574.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37993443
            Sequence Read Archive: SRR3737017
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminiclostridium sp. (human gut metagenome)
  ORGANISM  Ruminiclostridium sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Ruminiclostridium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_47745_length_1002_cov_5.0000"
                     /isolate="gSpreadC_ID_896"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:2053608"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-27"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          340 a          198 c          214 g          250 t
ORIGIN      
        1 ccctaccgct actcattgta tcattatccg ttatcgctgt cgcagccgtg acaattattc
       61 ttttgctgtt cacaaacgcc ggtgataccg caggttcatc cggttatatt atgcctgcaa
      121 agcctcgctt tatttactca aagaacaatg tgctgtatat ctatgacgat ggcacggcga
      181 aatgtataag cgagcttccg gcagaagatt atacactgac aaatgacggc aacaaattat
      241 tttatctttc cgacaacagc ctatggtgcg gtaatattga aaacgaagaa atcaaaaccg
      301 agaaaatagc cgatgaggtt gccttttata ccgtaaactc cgatggcagc aaggcgatgt
      361 attccgcaaa aaacggcgat gttttcgtat actcaaaggg cagcgaagcc aaaaagatcg
      421 gtacaaactg caccaccgaa tatatgtcac tgtatatgag cgataatgcc gataaagtta
      481 tgtatataac aaaaaacgag cagttgaaat actgcgatgg tacaaacgga aaagtaattt
      541 ttgttgatta tgtaaacagc tataccataa gatgttcgga aaatctgtca acggtaatgt
      601 acgggaaaac cgattataca accgattacc ccagacaaca agttgtagta tacagtaact
      661 tcggggaaaa taaagaagta ttcgggggat acggaatttt cagtgcatat cctgaaaaga
      721 acataatgta ctacaccgat tttcgcaacg atctatacta ttacgaaaac ggcaagagcg
      781 taaagataag ggatagcgac aattcgggaa tatccgcctc agatgaaccc gtgcttgtta
      841 caagcggcgg aaaaggcttt ttagttacca aaaaagataa aaccgaagag atagaattca
      901 gtcttaaggc aagctgtgtt ttgattacta tgcgtgagat cagtgctgac ggaaacacgc
      961 ttatctttac cgcaagcggg gacggcaatt caaagatata ca
//
