LOCUS       DAWBGR010000001        49468 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_323_length_49468_cov_4.352478, whole genome shotgun sequence.
ACCESSION   DAWBGR010000001 DAWBGR010000000
VERSION     DAWBGR010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 49468)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 49468)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..49468
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_323_length_49468_cov_4.352478"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    19012..19021
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        11753 a        13741 c        11852 g        12112 t
ORIGIN      
        1 tcgccagacg ctctttgtgc ggtattgcct tagggaaaca ctgaataaag cttgtgcaat
       61 gctgccttta cttggatttc tttacagctg caacaatcgc ctttacgatc tgtaccagaa
      121 tcatagagcc aaagctgaaa ccataaattg caccgatcag tacaccggac atgccgtttt
      181 cgatctggaa caatccatgc agtgccggaa tccacagcac agcgttaatg agtaccatgc
      241 ctgctgcaaa cgccagaatc gcaaacggat tattccacag acgcttggaa aagatcaccg
      301 gcttctctgc cttgcagctg aaaccgtgga acagacgtgc catgcacagt gtggcaaatg
      361 ccatggtcat agctgcaccg gcattgtgtg ctccctgata gaaatagccc agcaggaacg
      421 caccaatggt ggcaatgctg ataatcacgc cggaatagcc cacctttgcc agaaagtcct
      481 tggtaaggat cgactcattc gccggacgag gctttctctt catcacgtca tcggaatggg
      541 gttccagtcc cagtgcaatt gccggcagag agtctgtcag cagattgata aacagcaggt
      601 ggatcggagc aaaaggcacc ggcagccctg ccagcgagca gaacagcact gtaatgatac
      661 ccgccagatt gccggacagc aggaacagaa ttgccttctt gatgttctcg tagatatttc
      721 tgccattctt cactgccttg acaatggtgg cgaaattgtc gtccgtcaga atcattgccg
      781 cagcgtcctt ggaaacctct gtaccggtga tgcccattgc cacgcctaca tcagcctgtt
      841 tcagtgccgg tgcatcgttg acaccgtcac cggtcatggc aacgatattg ccacgcttct
      901 gccatgcacg gacaatgcga atcttatgct ccggtgtcac acgggcataa accgatttgt
      961 ctgccacaaa gtccaccagc tgctcgtcgg tgtaagcgtc cagtgccgca ccctctacag
     1021 cttcttccgg cttttccaga atgccgatct ccttggcgat cgccgaggcg gtgacaatgt
     1081 ggtcaccggt gatcattacc ggcttgattc cagcagatct gcactctgca actgcctgtt
     1141 tggattcctc acggggcggg tccatttcgg caatcagtcc cagataggtc aggtcgtttt
     1201 cgtcctcaac gctcagcgta tcaccggaaa acggctttgc cgcaaaacag agtacacgca
     1261 gaccttgttt cgacaattct tccacctgtt ccacaatttc ctgcttctct gcatcagaac
     1321 atgtcatgcg gttcagcagc acgtctgttg cacccttggt gtacattttc cgcacatcac
     1381 cgtgctggtt caaagtagac atcagcttgc ggtcggagtc aaagggaatc tcgctgatgc
     1441 gggcatgttc cccacgcatt ttctcatcgg acagcccgca gttctgggca aaccgcacca
     1501 gagccgtttc cgtcgggtca ccgatctcag taccgtcctg acatgtggca tcactgcaca
     1561 gcacggaagc cagaatcagc tcctgttcct ctggattttc cggactgaca tcttcggcgg
     1621 caatgatatg atcctgcacc acgattttcc ggacggtcat cttgttctgt gtcagggtac
     1681 cggttttatc cgaacaaatg acggaaacag aacccagtcc ctcgactgcc tgtagctttc
     1741 gcataatggc gtgttcctgc gacatcttct gagtaccaaa cgacagcacg attgtgacaa
     1801 tggaactcaa cgcctccgga atcgctgcaa ctgccagagc aatggcaaac atcagggaat
     1861 ccataactgc actgaaattg attacatcaa cgcgaatcat gctcagcacg aacaccagtg
     1921 cacagacgat catgataccg atagaaagcc gctttccgaa ctggtccagt gtgatttgca
     1981 gcggggtttt ccgagcctct gcgttctgga tcagggaagc gatctttccc acttcggtct
     2041 gcatgcccac ttctgtaacc acgtacacac cgcgtccgta ggtgacaaat ccaccggaat
     2101 acaccatatt cttccggtct gccagcggca cttcatctgt aatggtgtcc agagattttt
     2161 ccacattggt gctttcgccg gtgagggcac tttcgtttgt ctgaagtgat gcacagtcga
     2221 tcagtctgcc gtccgcgggg atctggtcgc cggcttccag cagaactacg tcaccaacag
     2281 tgatctcctc tgccggcagg attgctgtct gtccgtcacg gagcaccttt gccgtaggcg
     2341 acgacagctt tttcagattg gagagcgact tttccgcctt gacagtctgc accgttccca
     2401 gaatcgcatt catggtgatt acggcgataa tgacaatgaa gctttccaga tcgccggtaa
     2461 atgcagagat cagggcggcg atgatcagaa taatgaccag aaaatccttg aactgttcca
     2521 gaaaaatcag ccacgtcggc ttttttttcg cctctgcaat ggcatttttc ccatactgtt
     2581 ccagatgttt cgccgcagtt tccgacgata gtccctctgg acatgtgcct aactcctgac
     2641 agagttcttc gggtttctgc cgataatgtt cgttcatgta caacattcct ttcctttgta
     2701 gcatacgggt cggacaggca cggacagaaa aaaacggcaa gaccatacgg aaccgacaac
     2761 tctcctgtca aacgtgctcc gtaaaagtct tgccgtttag atatgaagcg ggtcattttc
     2821 tcaaacgacc gtactgacgc cgcataaaca ggagaatctc ctgccagcta ctctctttta
     2881 ctcttaatat tatacccaac ttcatgaatc ttgtcaagta aaaagaaaaa agttcacaaa
     2941 agagccgatg acacgcttcc gcatcatcgg ctctttctat atgatattgc tgtctgtgac
     3001 agacgttttt agtcgtttac cttgcgggta taatccggca gcagcttcgg agaaacttcg
     3061 tccggctgga taccagcgtc agccagttcc ttttcgaact ttgcaacgtg atccagagtc
     3121 agcttgccga cttcaacagt ctttgccttt gcagcagcgt acttaccagc gttacggccg
     3181 aatacgataa cgtccagcag ggagttaccc atcagtctgt tttcaccgtg tacaccacca
     3241 gcagcttcgc cggcaacgaa caggttttct acgcaggtct ggctgtctgt gccaaccttg
     3301 acaccaccgt tctggtagtg cagagtcgga tagatcagca tcggctgctt ccgaatgtcg
     3361 atgccatatt tcaggaacat tctcagcatt gccggaatcc gcttttcgat cgtgccttcg
     3421 ccgtgaataa tgtcgatcat cggagcgtcc agccatacac cctgaccacc ggtcggagtg
     3481 tctacctgct tgccacggcg gcattcacga ataacggtag atgcggtaac gtcacgagtt
     3541 tccagcgggt tcatgaagac ttcgccgtct acgtttacca gctttgcacc cagagaacga
     3601 accttttcgg taaccagtgc accgaagatc tgttccggga atgcagcacc ggtcgggtga
     3661 tactgcaggg tgtactgctc acgcagcgga gcacccacac gataagccag caccagaccg
     3721 tctgcggttg caccgtagtg gttggaagtc gggaatccct gatagtgcat tctgcctgca
     3781 ccgccggtag caatgataac aacctttgcc ttggcgatca ggatctcgcc ggtttccatg
     3841 ttcatcagaa cggcaccggc acagtgaccc ttgtcgtcca tgatcagttc gattgcagaa
     3901 gtgaaatcca ctaccggaat cttacggttc agcacttcgt cacgcagggt acgcatgatc
     3961 tctgcaccgg agtagtcctt cgcagcgtgc attctcttac gagaagtacc gccgccgtgg
     4021 gtggtgatca tgttgccttc tgcgtcctta tcgaactcaa cgcccagatc ctgcagccat
     4081 tcaatgccct ccggagcctc ggtaaccagc ttataaacca gttccttgtc cgcagcaaag
     4141 tgaccaccgc cgtagcagtc gaggaagtgc tgtgccggag aatcgttcgg cttatccgca
     4201 gcctgaatgc cgccttctgc catcatggtg tttgcatcac cgatacgcag cttggttacg
     4261 atcataacct tttcgccggc attgtctgct tcgatcgcag cagatgcacc agcaccgccg
     4321 ccgccgatga tgagcacgtc ggtttcataa tccggacagg aaagatccgg caggttgtcc
     4381 ttcaggcggc tgtcagactg gagcattttt accagttcct tcggagcctt ttcgcccttg
     4441 ttcggaccaa cagtcagcac gtcgaactga tcttcgcgat agtccgggtg gttttctttc
     4501 agaacctgat ccttttcctc tgcggtcatt cttctcggtt ccattgcagc attttctgca
     4561 cgctttgctt ctaccttctt aatggattcc agcatttctt ccgtatacat gataccgcac
     4621 ctccttactt ttcaatctca cgattgttgt acttttcacg aatctccgca tcgctcagtg
     4681 ccatatttgc acggattgct tcttcgcact tgccttcttc gatttccttg acacgatctg
     4741 ccagatgctg acatttcggt tcaatgtact taccgttcag gcggcgagcc agcattgcaa
     4801 cctgcgggtg cgagatacca gccggacagc gggaagaaca tacgccgcac ataacgcagt
     4861 cgaaagattc ttctgcacac tttgcatatt ccgcacgctg tgcatatgcg atatactgca
     4921 taacgttcag accctgtgta caagcattgg tacaagcgtt acagccgatg caggaataga
     4981 tttccggata gttcttcatc attacggttt cgtccggacg aacctcgttg atgtcataga
     5041 tctccttgat cagcgggaag aacggcagct gtgtgatgta catgccttct accacctgtg
     5101 tggagcatgc cagacaggtg tgcagttctc tgtcaccctt gatccggtaa attgttgcac
     5161 aggcaccgca gaaaccattt cggcagccgc agccgcgaac cagtttatag ccagcgtatt
     5221 ccaaagcctt catgatggtc agactggcag gaacctcata cttcttgccc atcacgaaca
     5281 cattaaccat ctgttccatg tgaattctcc tttaccatta atattcgttc ggcagttcgg
     5341 acagctcatc aaagcggaat accggaccgt ccttgcagac gtacttgtca ccgatattgc
     5401 agcgtccgca cttgccgata ccgcatttca tacgcagttc cattgtggta tagacctgtt
     5461 cttcttggaa gcccagttct ttcagaccgt ccagtgtgaa cttgatcatg atcggaggtc
     5521 cgcagatcag aacagtacgg ttcaggtccg gcttcagttc cttgacatag ttcggaataa
     5581 agccaacgtg accgtcccag ccctcctgtt cacggtcgat ggtcagatcc accttgcagt
     5641 cctgactcca ttcgtccaga atttcctgat agtctaccag atcgtccttg gaacgggagc
     5701 cataaatgat atgcagagaa ccataagcgt caggattctc acgctttttg tcacggcaat
     5761 agttgatgac ggaacgcagc ggagccagac cgataccgcc ggcgataaac agcaggtcct
     5821 ttcctttcag accatagttg ccattttccg gtgtcggaac ggtttccacc ggaaagttct
     5881 ttcccagcgg acctctgacg gtgacttcct gtccaacgtc catcatgtgc agccagcttg
     5941 tcagacagcc gcacttcttg atcgaaaatt ccatgaattc cttgtttgtc ggagaagatg
     6001 taatggagaa cataccttcg ccaatgcccg gaatggaaac catcgcacac tgaccgggca
     6061 tatgctcgaa cagtttgttt ccgtccagac cgaccacgcg gaatgtcttg acatcgggag
     6121 tatcctgacg aatgtcagtt accacgccga ccttcggaat caatgcttct tgtttcatgc
     6181 gttaccctcc cctaaagtct tcatgacctt tacaatgttc atggaaatcg ggcagctctt
     6241 catgcagcga ccgcagccaa cgcagccaaa tacgccgtcg ttgtttgccg gatagtaaac
     6301 cagcttatgc atgaaccgct gacggaaacg ctccagctgt gtgggacgcg gctgaccgcc
     6361 ggctgtcttg gtaaagtcag cgtacataca gctgtcccag cagcggaacc gctgtacgcc
     6421 gtgaccggtg tcatagtcgc gaatgtcata gcactgacag gtcgggcaca cgaaagtaca
     6481 ggtgccgcag cccagacatg cttctgacag gcttgcccac ttatcggaac tgaacagttc
     6541 catcagctta tctccgccaa aggagtcggt agagaggttc ttcagcggca gcttttccag
     6601 aattgccttg gtctttgcct tggattcttc cgcagcatcg tctgcggcat cttccagcat
     6661 cggcagagaa gccagcagag cctcaccctt ttcggtgttt gcctgcatca gcactgcatc
     6721 atctgtcagc cagcagctga catcaccagc cggttctgcg gcatcgatac cgaataccga
     6781 gcagaagcag gtttcactgg gcttgctgca ggacatggtc attaccacgc cgtgttcccg
     6841 acggttctga tagtaggtat ctaccggttc cgacagatac acgctgtcca ggatcttgaa
     6901 gcttgccgca tcacaggcac gcacgccgaa gatcacaaag tcctcgcact cagtacgaat
     6961 atcatcgact tcgatcttct tgccgttgac cttgaagttg accatgtttt ctacctgcgg
     7021 gaagaaaaag tccttggcac tgcggacagt gttgactgcc tctgcagcat actttgcttc
     7081 cggtgtccag acgctgtact ttacaccgcg tttttcatcg gtgccgtcag caaccggcag
     7141 atagagtttc tttgcttctg cgatggcagc gaacaaagcg tccagatttt ctcgagatac
     7201 ttttttcatc acttgctgcc tcctttataa accacattcg gctcgcagtc ttccttggtg
     7261 aagttcacca gcggtgcacg gctgccttcc tctgcaccag cctgatattc accataataa
     7321 gtgttaatat ccttgatgaa cttccggttc agcaggtgca gcggaatgtg ctgcggacat
     7381 actctggagc actcaccgca gtcggtacag cgacctgcta cgtggaatgc acgaataatg
     7441 tggaacatat tttcttcgaa ggagtctgct gctgccttct gggaaacacc agatgccgga
     7501 ttgtcaaaga cgcactgttc gcagctgcat gccggacaga tgttgcggca ggcgttgcag
     7561 cgaatgcagc gggacagttc cccacgccag aatgcgaatc tttcttccgg tgtcatagcc
     7621 tccagctttg cgaccatatc gaaacgaccg gactccggat tttcctcacc gttttcgccc
     7681 agcatctcat cgtagacaac aaccttgtgg ctcttgcaga cttcgcagcg ttccagcagg
     7741 cagtccttct tgctgacaga aacttcaccg tacatggttt ccgcctttac ggtgtcaccg
     7801 tcagtggtga tgcccagaat gccctcggca ccggcttcac gtaccttatc cggatccagc
     7861 ttgccgtcac agggaacgcc gatgatgtaa acgttttccc gcttgatgcg gtgttctgtc
     7921 agcagctggt tgaagctata ggaatcacac ggcttcagga aaaccagtgt cttgccctcc
     7981 agcttagaca gtttgatcag atacttcgac agattgctgc cgcagaaatc gtgatataca
     8041 aaatcctttt ccaaagcttc tgcagaatcg aacacagccg gtgtgggatc atagacaaac
     8101 tcaccgtttt tccagcccag cacacgaaca actgtaccgt ctgccaacag ctcggaggct
     8161 cttttcatca tttcgctttg catctcagat cctccagtct tttgttctcg cccagtgcag
     8221 tgacagtttc tacaaactca ttcatggttg cagcgaactt tgcaccttct gcggcggata
     8281 cccattctac acgggtacgc tctttctcga tacccagata atccagcatc gagaacagca
     8341 gtgtcattct gcggcgggca tagtagttac cagtggtata gtggcagtca cccgggtgac
     8401 agccgcagag gatcacgccg tctgcaccac gctggaatgc acgcagaata aagagtggat
     8461 tgacacggca ggagcaaggt acacgaatga tcttgacatc tgccggatag tttgaacggt
     8521 ttgtacccgc aaggtccgca cctgcataag aacaccagtt acagcagaat gcgacgattt
     8581 tcggtttaaa ttcctgattt acttgcatat tgcatctacc tccgccataa tctgaccggt
     8641 tgcgaagccc ttcagatcca ttgcaccgga cgggcatgca acggtacaag caccgcagcc
     8701 ctgacatact gcctcattga caactgctac gcggcgaatc atcgagccgt cctgacggcg
     8761 gctgaagtcc ttctcctcat aagtaattgc accgtacggg cagacatttg cacaagacga
     8821 gcaaccattg cacttcatgg gatccgaatg ggatacgcac gggttgcagg acagcttgtc
     8881 ctttgccagc agaccgataa ccttagaagc agcggcacca gcctgtgcaa cggtttccgg
     8941 aatatccttc ggtccctgac aaacaccgga aaggaataca ccggctgtcg gggattctac
     9001 cggacgcagc tttgcgtgag cttctgtgaa gaagtcgttg gtatccatgc ttgcagtcag
     9061 catggttgcc agcggacggg cactcttgtc tgcttcgatc gcagcagcca gaacgaccat
     9121 atctgccttg atgtgcagct gttcgttgga gatcagatca gaagcctgaa catccagtgt
     9181 gccgtcagac tgcggagcaa ccttgccgac cataccctta atataatgta cgccatactg
     9241 ttctacggca cggcgataga actcatcata gttcttgccc ggtgtacgaa cgtcgatgta
     9301 gaatacatag acatcggtat ccggatattt ttcgcggcac agcattgcgt gctttgcggt
     9361 atacatacag cagatcttgg agcagtattc cttaccgcag ccatgctgag aaccggattc
     9421 acggctgccg acgcactgta cgaataccag tgtcttcggc tgcttgccgt cggacagacg
     9481 ctgcaaatga ccttctgtcg gaccgtctgc cttcaggatc cgctcgaatt ccagagaagt
     9541 gacaacgtcc ttgctctggg aatatgcgaa ttcgtcatag ccgtcaatgt tgatcggatt
     9601 gtaaccggta gcggcaacga ttgcaccgta cttttcttct acgaaagttt ccttctgctc
     9661 gtagtcgatc gcaccggcag agcaaactgt cgaacacaga ccgcacttgc ccttggtgag
     9721 cttgatgcag cgtgtggagt ccagaacagc aacgttcggg attgcctgtg caaacgggat
     9781 atatgcagcg tgacggttgc tcagtcccag attgtaagcg tccggaatgt tctttaccgg
     9841 acacttttcc gtacatgcac cgcagccggt acacttatcc tcatcgacaa aacgagcctt
     9901 acggcgaatc ttgacgctga agttgcccac gaaaccggtc actgcttcta cttcactgta
     9961 ggagtagatc gtgatcttgt cgttctgtgc acattctacc attttcggtg tcaggataca
    10021 ggacgagcag tccagtgtcg ggaatgtctt atcgatctgt gtcatcttac cgccgatggt
    10081 cggttccttt tcgacgatgt ctaccggata gccggcttct gcaatatcca gagcagtctg
    10141 gatacctgcg ataccgccgc cgatgaccag agcacgcttg gttaccggac tttcgccggc
    10201 gatcagcggg gtattcagca gaacctttgc aattgctgca cgtccgagga cgattgcctt
    10261 tgcggttgcc tccggaatat ccttgtggat ccacgagcac tgttcacgaa tatttgcgac
    10321 ttccagcatg taggggttca ggcctgctgc tgcaacagtc ttacggaaag ttgcctcatg
    10381 catacggggc gaacatgaac atacaacaac gccctccagc tgatactcct tgatcgcatc
    10441 tttgattatg ttttggcctg cctctgagca catataggga tagtcggtcg cataaacgac
    10501 tgccggctct tttccaagag tttcagcaac agtttttacg tcgacggtcg ccgcaatgtt
    10561 cgtaccgcag tggcatacaa aaacacctat tctccgcatt tttatgcctc cttatttgct
    10621 gtatttccgg aatgcactga cgattgcctg ctgcggcaaa tcgtcgtcca gcatttcggg
    10681 aatctgctcc gatttcaggt gattctgtcc ctgctgacga attaccgcca gtctgactgc
    10741 ttccaccagt tttgccggtt ccacgtcacg cgggcagcgt tctacgcagg caaagcagga
    10801 caggcatgta taaatggact tcgatgccat cagcttttcg atgtcacctt tttccaccat
    10861 atatacaaac tgatgcggat gatattccat tgcatcataa gacggacagg taccgctgca
    10921 ctttccgcaa cgcatacact tcagcggatt cacgccgctg gtcgtcagaa cgagttcttt
    10981 caaatctgcc attttcgttc ctccttattt cagaccgagt gcttccgcca gcagctcggt
    11041 aaagtatacg accggcagat ccgaaacgcc ggaattcttc tggaggttgt acagacaaag
    11101 cggacatgcg gtgatcacca tttctgcacc gtggtccttg gcactggcca tcaccttgtt
    11161 gcttctggac tctgccacag acttttcgtc cagtgtcaga tagccgccgc agcattcgtt
    11221 ccgcatcgga tacagtaccg gtgttgcacc aattgcacgg ataaagtctt ccagaatctt
    11281 cggattctcc ggattatcca tctgcatggt ctttcccgga cggagcagaa gacatccgta
    11341 atatgcggcg atcttcttgc ccttcagcgg atttaccact ttctttttca gttcatcgaa
    11401 gccgaccttg tcccgcagta cttccagata atgcagaact tcggtttcgc cctgatacgg
    11461 ctctgccagc ttcagataat tgtttgcttt cagaacaatg ttgtcatcgg tctgcatatc
    11521 atggttgacc tgcttgatga cattatgaca ggcagaacaa agggtaacca gaggctgtcc
    11581 cagttccttt gcttttgcca gtgcacgaat cgatgagagc ttggttgcga tctcattctt
    11641 cgccattgga tacacaccgc cgcagcactg ccagtccgga agttcttcca gagtaaagcc
    11701 cagtgcttca gcagagatcc gagcatagtg atcgaggtct ttcgccttgg tcttcagtgt
    11761 acagcccgga aaataactgt atgtcatagc aaaactacct ttccttcctg tttttttgca
    11821 ccgaagtgca gacggttcac gccatctcct cgggatggaa tacttcgtcg aaccgttcag
    11881 ctgtcaggaa tcccagctct acgcaggctt ccttcagcga gatgttgtca tggaacgcct
    11941 tctttgcagt ttttgcagca ttctcataac cgatgtaggg gttcagtgcg gttaccagca
    12001 tcagagaatt gtgcaggtta tggtgcatct tttcgcggtt tgcacagatg cccactgcac
    12061 agttcttatt gaaggagaca atggactctg ccagcagacg tgcagactgc aggaagttat
    12121 acgcacaaac cggcataaat acgttcagct caaagttacc ctgagaagct gccatgccga
    12181 ctgccacgtc gttgcccatt acctgaacag caaccatcgt tgcctgttcg cactgtgtcg
    12241 gattgacctt gcccggcatg atggaagaac ccggttcgtt ttccggaatg gtgatttcac
    12301 ccagaccatc tctcggaccg cttgccagcc agcgaacgtc gttggcgatc ttcatcatat
    12361 ccgcagccag agccttcagg gcaccgtgtg caaatacgat ctcatccttg ctggtcagtg
    12421 cgtggaattt gttggaagcg gtgacgaagt ccttgccggt cagttcagaa actgccttgg
    12481 caacttctac atcaaagccc tccggtgcat tcagaccggt accaactgcg gtgccgccca
    12541 gtgccagtgt cttcaggtac ggcaacgcag aaacgagcat ctcgcgatca cgctcgagag
    12601 aagtacgcca gccgctgatc tcctgcgaga actggatcgg tgttgcatcc tgcaggtggg
    12661 ttctgccgct ttttacaatg cccttgtgtt cttcttccag acggcggaag gtagccacca
    12721 attcgtcaat tgccggcagt accttatcct ctacagccag aacagcagag atatgcattg
    12781 cagtggggaa tgtgtcgtta gaagactgac tcatattgac atcgtcattc gggtgcagga
    12841 tcttctttcc ggcaacttca ttaccgcggt ttgcgataac ttcgttggcg ttcatgttgg
    12901 actgtgtgcc gctgcccgtc tgccatacaa ccagcggaaa atgctcgttc aggctgccgg
    12961 agatcacttc gtcacaggca gtggagattg cagaaagctt ctcctctgtc atacgctccg
    13021 gacggagctt gtggtttgcc atagcggcag cctttttcag gataccgaaa gcgtgtgtga
    13081 tctcgcgggg catggtttca atgcccacac cgatcagaaa gttctcgtgg ctgcgttctg
    13141 tctgtgctgc ccagtactta tcggcgggca cctttacttc gcccatcgaa tcgtgttcaa
    13201 tgcgatactc catgttcatc tctcctattt tgaaattttg atgatggtga ttgtgtctgt
    13261 ttctgctgtg cggcagaaac gcttttgtgt ccggtaaaaa gactgtcctg ccctcggctg
    13321 cagtccgtca gttcgggctg cttgtccgca gtctttctgc ggttccacag ccggatcccc
    13381 gtgccgatgc aatgcaccct ttcacggaaa catactttta tgacagaaca gcctttcttc
    13441 gctgtcggaa ccgctgaaag cggctcctga tcccgtcctg tccgaacgtc attttcacgg
    13501 cggcacggca caaaaaaatc tgctgtacaa cacggcacag caccgccata gtgaaactgt
    13561 ctgtgacagt tcccgacaga aaaatcaaga attctccccg aaagccgtcg cttttccaga
    13621 gaaaaactac atgatgtact atctatgata ttataacata ttccgcctga tttttcaagg
    13681 gacaatcgca aattttgtat gaacaagcgg cataacagcc tccggtgtcg attcccgaat
    13741 ggtaaaattt ctgtcacacg acaccattcg ggaaatttca tgtcattttg cattatacca
    13801 tttctttgtg aaaaaatcaa cagcttttga aacacctcat gttttgcaaa gtttttcgcg
    13861 gaaatacgct gtttttttgt catgcccctt tgaaaaaact ttgtttcccg aaacgagaat
    13921 gtggtttttc atagttagta taagctaact ataattttac agaaatcgca gccgattttc
    13981 cgccggtttg aaaagttgtc cggtttcttc aaaaaatcta acgaatccac ttgtcttttc
    14041 ccgaaaagcg tggtataatg cagttgttcc gaaaaacaga acaacggagg tataccccta
    14101 tggaaacatt ccacaaccgg ctgcagcagg cgatgcagca aaaacacatg acgcagtctg
    14161 acttgtgtgc caaaaccggc atttcccgtt ccaccatgag ccagtattgt tccggctctt
    14221 tccgcccgaa ggagagcaag ctgcggctgc tggcgaaggc tctcggcgta aaggaagcgt
    14281 ggctgctggg ctatgacagc cccgaagaac cgcctgtttc atgggaggac tatcccaatc
    14341 ttcgcccgat tcagctgaaa cgctttccgc tgctgggtga gatcgcctgc ggcgaaccga
    14401 tttttgcaca ggaagatcgg gaaacctttc tgatcgctga tgcagaaatt gctgcggact
    14461 tctgtctgct ggcacggggc gacagcatga tcggggcacg gatctacgac ggcgatgcgg
    14521 tgtttatccg cagccagccc atggtggaaa acggcgagat cgcggctgta atcattgacg
    14581 acgaagccac gctgaaacgg gtctactacg acagagagca cgcacgctta cagctggtac
    14641 cggaaaatcc ggcatactct ccgctggtct atgtgggaga agaactggac aatatccgca
    14701 tactgggaaa agcagtcgga ttcatgagta ttttttaaga caagcaaaaa gcggcagaca
    14761 gttggggaaa ctgtctgccg ctttgttttg gaattgcaaa attacagaag cgggctgatc
    14821 gtccgcagga tcgcctgcca gagcagactg tgccggcgac gcttgcagtc ctgaagcgtc
    14881 acacggcggc ttttctcaat ggtcacaacg cagtccagct tcatcttctg caacgcactg
    14941 cattccacca gcatcgccgc acactccatg tgcaggtaca aactgcgata gtccatgttg
    15001 atcgttccta caatgccgat cctgtcatct accagatagc ttttggcatg gataaatccc
    15061 ggcgtatact catagatccg cacccctgcc cgaatcagcg gctcataata ggaacgggtg
    15121 agagaataca ccacacgctt atccggaatc cccggtgtca caagccgcac atcgaccccg
    15181 cgttgtgccg ccatgcaaag ggcgtgctgc atttcgtcat cgattgccag atacggcgtg
    15241 aagatataca catactcttt cgcctgatac agcagatcca gataaatatc ctgtgccagc
    15301 gtttcatttt caaagggcga gttgcagtag ggctgcacat agccgtctgt gggaaacggt
    15361 tccggatgat accggtgtgg caggtacttc ttgcagttgt cattggtgtt gtagaatgca
    15421 ttccacagtt ccaaacacat cagcgtaagg ctgtacacgc cctcgccata gagccgcagt
    15481 ccggtgtctt tccagtgccc aaaccgctgt ttctggttga tatactcatc tgccagattg
    15541 aagccgccgg tataggcaat atagccgtca atgacagtga tcttccggtg gttccggttg
    15601 ttcatcaccg cggaaagtgc cggtcggaac gggttgaacg tcagcacatg gattcccgcc
    15661 gccgcaagtt ctttccggaa attcttcggc agacagccca cgctccccag atcgtcatag
    15721 gcgaagcgta cttccacgcc ggcggctgcc ttttctttca gaatggcttt gatcgaatcc
    15781 cacatgactc cctctgtcac gataaaatat tccataaaga tataatgtct ggctttccgg
    15841 agatcctcca gcagtcgggg aaacgcttca tctgccaaag aaaaatattc caccttggta
    15901 ttctggtaca tgggaaaacc gctgctttcc agatagaaac tggtttccgc catgcgtacc
    15961 ggaaacgccg ccgcagaaca gcccacagag ggacgaatcc cgatcacccg cctgccgtgg
    16021 tgcagctgcc ggcggatccg ccgcgaggga cgcttgtccc cgaacagcag atacatcatt
    16081 ccgcccaaca gcgggaacgt accgataaac agcatccaga gaattttata agagggattt
    16141 ccggacttgt tgatgatata caattcgatc gccagactca gtccgataaa tgtaatattg
    16201 atccacgttg catactgtgt cagccgcaga aacaagatca caaaccacac cagctgcaag
    16261 acaatcagga atccgataat taccactcgg ctggtcaaca tgctgatcag ttttttcagc
    16321 tttcgccgca caacatcacg ctcctttccg gaattgcttc gggcaacact gcacagtctt
    16381 tatgcggtgt tgccttcatc tcttttagta tagcagattt ttcggaaaaa ggcaagcctc
    16441 acttttggag aaaaagcagg gcggtttgct gatataagaa aacatatcgg acgagaaact
    16501 cgttgacttc cattggcagc atcaaaaaat gcagaaataa aaaatgaggt gaagcaacat
    16561 ggatacagat gaatttattt gcagggaatg tgatggagat ttatctaatt ttgcaaaaag
    16621 agatgcaaaa tatgcaagct atacacagaa agatttcgat gcagaattcg aaaaatttaa
    16681 aaaagaattt gcagaaaagc acaaaaatga tgaaagcacc tcattttgaa ttcccaaaag
    16741 ttagaccaaa aaaatctaac aattaggagc agttcaaaat gaggtgcttt tttcacgttt
    16801 aattttcacg tttaaatggt aattctatgt tttccttagg aaacgctgca taaagcttgt
    16861 gcgtaagatg cgtagtcaga tttttcggca gattgcacag aaattccgca ggcatacttc
    16921 atgtatggca agggatttct gggcaatatg acggaaaatc tgcaagcaga ttgcgtgcaa
    16981 agccgtcagg cgggctttat gcagtgtttc ctttactcgg catagccgtc ggggtgatgc
    17041 tggtgccagt tccatgcact agcaatgatt tcttccagac tgtcatgtac cggtttccag
    17101 cccagcacag cctttgcctt ttcgctggaa gccaccagcc gtgcaggatc gccggcacgc
    17161 cgcggggttt ccactgcggg aatcgggtgc cctgtcacct tgcgggcagt ttcgatcact
    17221 tcccgcacag aatagcctac accgttgccc agattgaaaa tgctgctttc cccgccgttt
    17281 tgcagatact gcactgccag aatgtgtgcc tgtgccagat cggtcacatg gatatagtcc
    17341 cgaatgcagg tgccgtccgg cgtgtcatag tctgtgccgt aaatggagat cgcttcccgc
    17401 ttgccgttgg gcacctgtaa aatcagcggg atcaggtggc tttccggatt atgtgcctcg
    17461 ccaatggtgc cgctggcatc tgctccgcag gcgttgaaat accgcaggga aacataccgc
    17521 agtccatgtg cgtttgccgt ccagtggaac atcttttcca ttgccagctt ggtttctccg
    17581 taagggtttg tcggcagtgt ccggtcggtt tccagaatcg gaatgtgctc cggttcgcca
    17641 taggttgccg cggtggagga aaagacgatc ttgtccacgt gatttttgac cattgcctcc
    17701 agcagcaccc gtgtgccgca aaggttgttg tcataatatt tcagcggatt tgtgacgctc
    17761 tctcccacca gcgagtatgc cgcaaagtga atgactgcat cgatctgctc ctgctgaaac
    17821 agattatcca gaaatgcaaa gtcgtgcagg tcgccctgat agaactttgc ccctgccgga
    17881 atggcttcgc ggtgccctgt caccagattg tccgccacga tcacatcttc tccggcacgc
    17941 accagttcca gtgccgtatg ggaaccgata tagccggctc cgcccaatac taaaatacgc
    18001 attgcaattg ctcctttcca aaagcttaaa ctctgacacc gcccatcgga cggacagaaa
    18061 gcttgtgaca gctgcccttt ccgaacagct ggttcataca ctgacaatat gcctcggctt
    18121 cttccaccgg cacaaatgcc tgaatagttc cggcaaagcc gccgccgtgt acccgcacag
    18181 cacccttttc accaaggaac tgtttgctgt accagatgcc cagcggaatc gcctgttctg
    18241 tgggcttctg gcaggaaaac agattttgca gcagttccgc agaagaagtg ccggactccc
    18301 gcaccagtgc aaagaacgca gacagatcgc cgtcttccag tgcctttgcc tctgcagttg
    18361 cccgttcgct ctctgcaaag aaatgtgccg cacggagaat ggcacggtcg gaacaggtct
    18421 ttctcagctg cggaatggct ttccggaact gctctgcgga aacctcccgc aggacagact
    18481 ttccgaaata agcggcaacc tgcttcatct ccgtcggaac cgccacataa tccggtgtca
    18541 gatcggcgtg gctgcccttg gtatctgtga tgcacagaca gtatcctgct tgtgcaaagt
    18601 caaaggtatg cttctgtatc accggctttt cagtgtccgc aaaatcgatc cagacgaaac
    18661 cgcctacgga actgaccatt tgatccatca agccgctttg cttgccgaaa tatacatttt
    18721 ccgcatactg tccgatcttg gcaatttcca ctgcaccggc ttttccgccg ttgtcatgct
    18781 ggtcgatgat cgttcccacc agcacctcaa acgctgccga ggaggaaagc ccgctgccgc
    18841 tgagcacctc cgaagtagag taagcgtccc agccgctgag ggatacaccc atatctgcaa
    18901 atttcgcagc gatcccccga atcaatgccg tggaagtgcc ttcctcctcc ggcttctttg
    18961 acaaatcgga aagctgcacc tgaatctggg aatagcccat agatttcagc cnnnnnnnnn
    19021 nttcagccgg atcacgccct cctgatgaaa accgacaacg gcgatcacgt ccagattcac
    19081 cgccgccgcc agcacgcagc cgtgctggtg atcggtatgg ttgccgccga cttcggtgcg
    19141 tccgggagcc gagaaaatgc cgacttgttt ccgctgggga tacagcttcg caaattcgtc
    19201 cagtgccgcc gcgtaccgtt tcttctggtg caccagcatg cctgcatcgt caccatacaa
    19261 ctgtttcagc ttcttgtcca gctttcccgc tttcagtgtt tgtttccatt ctgcaactgt
    19321 catcatcgtt cgcatatcct ctcatttcat ttttcccgta ctgtgcggaa tttcctcaaa
    19381 cttctgtaaa gtgcatggat accggcgtat aatcacccca tgccttcggc agcagcacgc
    19441 cgcctgccat gagtgccgca ccggtgtaga cttgttccgt gccgtccagc cggtactgct
    19501 tttctgcaca cagtccccgc agcttcacct gatgccgcag ggtgttggct tcggcgtgga
    19561 agatcatgcc ctgcaccagt atctcgctgc cgtcctgtgc cgcaaatgcc cagatcgctt
    19621 ctgcatcggt catggggttg gtcagacggt agtacctgcc gttgtggatc agcgggccat
    19681 aagccttgaa ctgctgaatt tgctctgtca cagcctgctt ttccgcatca gacagcagat
    19741 tcaagtccag ttcatagccg aagctgcctg ccatagccgt caccgcacgg gtttcaaacg
    19801 gtgtgatgcg accggtctgg tggttcggca cggtggaaac gtgagatccc actgcggaaa
    19861 cgggatagaa gaacgatgtg ccgtactgga tctttgtccg ttcgcgggca tcggtatcgt
    19921 cactgcacca gatctgcggg cagtagtaca gcatactggc atcaaatctg ccgccgccgc
    19981 cgctgcaacc ctcaaagagt acttccggaa acgccgccgt cagccgttcc agcagggcgt
    20041 acaagcccag cacatagcgg tgaggcatct ctccctgccg gtctgcgggc agagtttggg
    20101 tgtaccagtc actgatgctg cggttcatgt cccacttgac ataggaaatg tctgcactgt
    20161 gcagcacttc gctcatgaca cggaacaggt actcctgtac ttccggattg gtcatatcca
    20221 gcaccagctg ataccgtccc cgcatcggcg aacgctccgg aatccgaatc gcccagtccg
    20281 gatgtgcccg atagaggtcg ctgtcctcgg agatcatctc cggctcgaac cagatgccga
    20341 accgcatgcc catcgccttg atcttctcca ccagatcgtg cagtccgccg gagagttttt
    20401 tctcattgac gaaccagtcc cccaaaccag agcagtcgtc gtcccgcttg ccgaaccaac
    20461 cgtcgtccag caccagcata tccacgccga ggcgtgcagc ttcttcggca atgtgcagga
    20521 tcttttccgt atcgaagccg aaataggtgg cttcccagtt gttgatcagc acaggacgtt
    20581 ctgccagctg atacttgccg cggcagatgt gttcccgaat tgcccgatga aactggtggg
    20641 acaaagtttc cagtccggtg ctgctataag acagcagcac ttccggtgtg gcaaaacacg
    20701 cccctgctcc cagctcccag cggaacaggt cggggtggat tcccatgact acacgcacct
    20761 gccccagctg gtcgcactcg atcttggtct ggtggctgcc gctgtagacc agagccgccc
    20821 ccacgcaggc accggaggtt tccgtgcagt ccggtgtgca gagcagcacg gtgggattct
    20881 ggtgatggct ggacgtaccg cgggtgctgg aactctcctg aatgccgtgg agcagcggac
    20941 ggcgttctgc ctgccgttcc atggcatgtc tgccgtagaa gtgtatccac tcccagctgc
    21001 cctgtcccat gtccagacaa aggcttgccg ccttggtcag tgtcaccggt gcttcgccca
    21061 gattgcggat ctctgcacag cgtgtcacaa tgtccagttc cggcagcacg ccgtatttca
    21121 aagtcacttc caccgcagaa gccgtatccc gcagaaccac ttccagtgtt tccgcttcgt
    21181 cctctccggc atacaacgcc ggcagaccgg caatggcata cttgccctgt ttcaccgcgt
    21241 ggctgtagta ccgcagatcc agtgcacagc tgccgtcagc gtgtaccgct tccactgccg
    21301 gcacccgaaa atcgcctatg cctgctccgg catattccag cggcagggtg tccagcgaat
    21361 aggtgcggtc atttccggca tcatggggat ttccggaaaa gcctacgtcc ggatagtcct
    21421 gcaaatagga catgtcacag ccagtctttg caccgtacca gagatgccgc aggaccccgt
    21481 aggtgtccgc ctgcatctgg tacatggtat gctttgtttc cagtgtaaaa attcgctttt
    21541 ctgtattgat cttgattgcc atatccaaca ctccttcagg atacagaaaa ccgtatgaca
    21601 ttgcagttcc attcttcgaa tcgtgtcaca tcatacggtt ttctgtctat tctctattct
    21661 acgaaattta tgaaaaactt ccttaaccct ttactgcacc attggcaatg ccgccgatga
    21721 tctgcttctg acagaagatg tagaagatca ggatcgggat cagtgccagc aggtaagaag
    21781 caaacgccag attgtagttc gtgccgaact gggtctggaa gttcagctgt gccagcggca
    21841 gggtggtgtc ttcgctgctg gacatgatca ccagcggtgt catgacatcg ttccatgtgc
    21901 ctagagccgt cagaactgcc acggtagcat gcatcggctt catcataggg aaaatgatcg
    21961 tccagtaggt tttccatgtg gaagcaccgt caatgtttgc agcttcttcc attgccagtg
    22021 gaacgttttt cagatagccg ctgtacagca tgacgttcat gggcatatag aataccaagt
    22081 acagaatgat aacgcccaga cggttgtcca gtcccataat ggcggtctgc ttgaccagcg
    22141 gcatcatcag aatggcaaag ggaacgaaca taccgctgac gatgtaaaag tagatcagct
    22201 tatagccctt tttccgtgcc atgtttctgc caatggcata gcctgccata gagtggagca
    22261 gcactgccag cacaatggtc actgcggtaa tcagaaagct gttgcccagc gagtgccaga
    22321 agtttgtcac acgcattgcc tcggagaagt tgctgaaatt ccatgtgctg gggaacgaga
    22381 gtgctccggc aatgtcattt gtcatttcgg tagggctctt gaatgcgatt atgacggtca
    22441 tgtacaacgg gaacaatacc gtcaggcagc ccagaaacag cagtacggtc agaagtacat
    22501 tggtaccgcc gattttatgt gtctttttca tagctgttcc tccttcttac cggtaatctt
    22561 catctgaatg atcgagaatg ccacaataac aataaagaat actacggcgt ttgcactctg
    22621 gaatccgaac tgtccgccgt ccataccgtt tttgtagatc aggtaggaaa tggactcggt
    22681 actctgtgca ggaccgccct tggtcaaaga catgatctgg tcgaatacca tcatcatgtt
    22741 cttggtgctg agaatcaggt taatggagac gaacggcagc agcatcggga aagtcagctt
    22801 ccagaacttc tgccacgggt ttgcaccgtc gatcatgccg gcttcgtaga tgtccgccgg
    22861 aacggtctgc aaaccggaaa tgtagatgat cgtattcatt gcgactgcct gccatacgcc
    22921 gacaaccagc acgccgatcc acgcgtatct ctcactggca agaatactgt tttccagaaa
    22981 gccgatgtgc attgccttgc ccacagccgg aatgatgtag gtaaagataa agctgaaaat
    23041 ataaccgacg atcagtccgc ccagaatatt cgggacaaag tacacgccgc ggagcaggct
    23101 cttgcagcgg atcctgctgt tcagccccat tgccagcaac aggctcagac agttgatgag
    23161 aatggttccg gcaatcgcat acttgaatgt gaacaggtag gagtgcagga cacgttcatc
    23221 agaaaacagg tcgatataat ttcgcagtcc cacgaaatca aaggtaccat agccgcggta
    23281 gttggtgaag ctgtaaactg caccggtgat cagcggcagg gtgttaaaca ggaagaacag
    23341 aatgacaatc ggaatggtca ttgccagata ggttcgattg ttgctgctca atgcttttct
    23401 tttgctcatg atgcattctc ctcctcatat ttttgtactt tcgcgatcag gtcgcggtta
    23461 taccgtttcc attccttatc gaaacgcttc aggaacttgg cggtggagtc actgctgtcg
    23521 tccatgagat aagtctgtac cattgcgtcc acgctcatct cactgggata gtggtggtca
    23581 tggaagtcgg acattctgcc ctcatcgata taggaacgca taccgtccag catcgacggc
    23641 agagtgaagt cgccgtcctt acagggcact gcgttctggt tgtccagata gatctgaatg
    23701 gtttcatcat catacaagaa cttcagtact tcataaacag catctttgtg cttggtttct
    23761 ttcatcacgc agaactgcag gtcaacaccg gagttcagca cgttatccgc ttcatcctca
    23821 tttgcaggga atacaaacga gtcaatgttc atatccggat ttaccgactg aatttgcggc
    23881 accgcatagc tgccgatggg atacattgcc gattcgccac gtgcaaaagc agtgcaggca
    23941 tcgttatagc tgtacgcata gggattcggt tctgcgtaat ccagcaggac cttgtccttt
    24001 tcggcaactt cctgatatgc gtccgtaaag gtcgtctttc cggcgttgac atcgctgcag
    24061 gtggacggag aggacaaacc tactgccagt gcattccacg gtgccataca agtccatgtg
    24121 tccttatatc ccagatacag cgggtacaca tcagaggtgt tctgaatggt ttcacacagg
    24181 tcggtgaatt cgctccatgt ggcagggatc tcccagccgt tctcctcgaa catatccttg
    24241 ttgtacagga ttccggcaca gtttgccaca taaggcagtc cgtatacccc gtctttcgga
    24301 atgaactcca gttcatctac aatgtccaga tatgccggtt tgatgcctga cacgccctca
    24361 aaatcggaaa catccagaaa catatctgcg tccagaaagt tggagtagtt gatgtcgccg
    24421 ccgataccga taatgtccgg ataatccccg cggataaacc gtgttttcag cacagtcatg
    24481 gcttcattgg gcgattcaat ggtcagatga atatcatcat gggtcgcgtt aaagcgttcc
    24541 tccatttcct cgaaagccgc aacagcttcc ggtttatact gtaccattgt cacttccgtt
    24601 ttcccgtcat ttttcgatgt acctgcacag ccggacagcg gcagtgtgga caatacggca
    24661 cctgccaaaa tcaggctgct gactacatgc tttcttctca tgtgatcttc ctccttttcg
    24721 ggtaaaaccc tgtgtttttt gctgctttta ttataacata ccaatatgca actggtaaat
    24781 accacaaatc aacttatttc ataccatttt gccgcttttc ccgcaaatat caaaaaacag
    24841 gttgcatttt ggtatatttc gtgctataat gagtatcata tgaacaacaa aaaacatgaa
    24901 tggtacaagt tcagatgaaa tttatgacaa gaggtaatat tatgcagaat ttgatctttt
    24961 caatttttcc caatcagaac tttgtagacc tcggcttgtt ccagttcggg tgggaacgct
    25021 gtacaccggc acactctttc ggaccggctg cgagaaacca ctatctgttt cactatattc
    25081 tatccggcac cggcaccctg atggcagacg acagcaaggg tgtcacccag acctactcga
    25141 tcaaaagcat gcaggggttc atgatcttcc ccaatcagat caccacctat gttgccgaca
    25201 agcagctgcc gtgggaatac gtctggctgg aattcgacgg actccgtgtg aagtccctgc
    25261 tggacaccat cggactttcg ctggacaaac cggtctatca tgccaggaac aaaagcctgc
    25321 gggaggacat ggcaaacgaa atgctgtaca tctcccgcca caaagatgag tcgccttttc
    25381 atctgatcgg gcatctctat ctgtttctgg attatcttct gcgttctgcg gcagatgaac
    25441 agctggaaca cggcagcaaa ctgcgggaat tctacattca cgaggcactg acctacattg
    25501 aacacaactt tcaaaatgag atcacgattg aagatattgc cggtgtctgc ggactgaacc
    25561 gcacttattt cggcaaaatt ttcaaggaag cactggggaa aacgccacag gaattcctgc
    25621 tgaattaccg catgctcaaa gcggcagagc tgctgaagct gaccagcctt tctatcgggg
    25681 atgttggtct ggcagtgggg tatgccaacc agatgcactt ttccagagca ttcaagaata
    25741 actacggcat ttcccccaga gaatggcggt atcagaacca tatcaacaat accgttgaca
    25801 gcgacgaaga acatacacaa taaaaaaacg agcctgtttt caactgaaaa aacaggctcg
    25861 tttcttgtta tgcagagggc attgtctttt tccggaaagt tcgcagcaga atcggaacag
    25921 acaccacagc tgtaatgcat tccgccagcg ggaacgccca ccagaccagt tctgcaccgc
    25981 gttcatacat cagaaacagc agacaaaacg gaatcggcag cacaagcaga cgaagcagtg
    26041 ccagcagcag cggcgaaaac acacagcgaa agccctgcaa gatgccctgt atcagcacag
    26101 tgattcccat aaacacatag cccagcgtac agatacgcat agaccgcatg caaagctgct
    26161 gcacttccct actgccgtcg ctctggaaca aaccggagat cagtgctgca aacagctgaa
    26221 acaaaactgt cactataagc atcagtccgg cggcgtacag cagcccgtag cgaacacact
    26281 gccgcagacg tgctttttcc cgtctgccgt aatgaaacgc caccacagta atcagcgtgt
    26341 tgctcaagcc gaacgctgca aaaagggcaa attgctgaat cttataataa atgccatagg
    26401 cagcctgcaa tggctccgcc agatctccct gtgtgccgaa taccagattc acaaacagca
    26461 ccataacaga aagcaacccc tgcatcactg cggcaggaat cccgatggcg tagatgccgc
    26521 gaatcagtcc ccaccgagga cgaatgcgtt tccagtcctg ccgaatctcc cgatcccacc
    26581 ggtagtgaaa cagcattgcc atgaccagtg acgctacctg tcccacaacg gttgccacag
    26641 cagcaccgac aacgcccata tccaatccaa aaataaacag cggatccaac aggatattca
    26701 acgcggcacc agtgatttgt gcaattgtgg aaagattcgt gcgaccggtt ccctgcaaaa
    26761 aacgttcata gacagtataa ccgatactgc caaaggaacc catacagcag attcgcagat
    26821 actgttcacc catttcctgc cggagagcat tcccgtttgc ctgcatggcg ataaacggct
    26881 tcgtgccgaa aatgccgaac agcagaaaaa cggcatagat gcagatcgcc agaaaaattc
    26941 cgtttccggc aatacaagcc gccttttccg aatttcgctc gcccagtgtc ttggaaagca
    27001 gggcgttgat gccaatgccg gtgcccacgc caacagccac gatcagcagc tgaatggaat
    27061 aggaatccgt cagagcaagc acggcagtgg ctccattctt catatggctg acaaaaacgg
    27121 tgtctacaat attgtacacc gcctgcaata ccatagagag caccatgggc attcccatgc
    27181 ttaggatcag ggtgcggatt ggaaaatccg ccagtttgtt cggggttccg gtttgtgtcg
    27241 ccgagtgatt ttcttgcatg atttctaaac ctccaaaagt caatggcgac actgcacaaa
    27301 gctttatgca gtgttgccca atatatgcaa aaacggcgta aacccagtca gattctaact
    27361 ggatttacgc cgtttcgcta cacgttatca atttatttta tcacaccgca ccgaaaaatg
    27421 caagtgtttt ttacaaaaaa tgttacagca aagccgtcag gcgggcttta tgcagtgttt
    27481 cctttacgct tcgtcgtcca gaaccggaat acgggacagg atctccacgc cttcctttgc
    27541 cagcttctgc ttgaatgcct ctgcggtctt ctggtcgcag ccgctgatca tgcagccgtt
    27601 ttctctggtg ttcagcagct ttacgccgac ttcatctgcg atcttggcaa atacaccgct
    27661 gccgcaggga gaagtccggt agcagcgtgc tgcaaagaag tcgtcgatgg gagccacgaa
    27721 gtctgctcct tcttctgccg gttcccagct ctgggattct actgtatcgg tctgctttac
    27781 ctcgtcgatc aggtcaccca gtacggcact ggcagtcggc agcttgccgg caccacggcc
    27841 atagaacatg gtcttgtcca caccgtcgcc ccataccatg acagcgttga acacgccgtt
    27901 gacatgggaa atcaggctct cgttcatgac aaccataggc atcactgtag gcaggatctt
    27961 tccgctttcc agacgcttta ccgcaccaat cagcttcact gcaccgccga aatcctgtgc
    28021 cagctgcacg tcttccagtg tgatgttgcg gatacccttg gtgtatacct gttccggata
    28081 tacatgcttg ccgaatacca gcgaggacag aatgcaaatc ttccggcagg catcgccgcc
    28141 gtcaatgtct gcggaggggt cacgctctgc atagcccagc ttctgtgcca gtgccagtgc
    28201 atcatcaaaa cgcatcttgt cacagaacat cttctccaga atgaagttgg tggtaccgtt
    28261 cagaatgccg gcaactgcgg agatgtcatt ggcagccaga caggtgtgca gcggacgaat
    28321 gatcgggatc gcaccgccga cgctggcttc gaagaagaag ttcacgtgat gtgcctttgc
    28381 cacctgcaac agaatgtcgc ctttttcagc aaccagctgc ttgttggaag tggcaacgct
    28441 cttgccggct tccagacaac ggcgaacgaa gtcaaacgcc ggattgacac cgcccatgca
    28501 ctctgccacg tagccgacct ccggatcgtc gagaatcacc tgaatgtcgt gtaccacgtg
    28561 gtcaccgtac ggatcatccg gaaattcccg cagatccaga atatatccta tttccagctc
    28621 tgtgccggta tttttttcga ttttcttctg attgcggtag aacagttcca caacaccgga
    28681 accgactacg ccataaccca atactgcaat tttcttcatt actttacttc ctccaagttt
    28741 cagcagactt taaaaatccg ccgtttattc gccggacagg agccgcacct ctacaacgcc
    28801 ctgcagggca gagatcttgt gcgtaatgtc aatgggattt gccataccct cgcccatttt
    28861 cagggacaga gaaacctgtg ccacgccgtc caccggaatg ttctggttga cagtcaggat
    28921 attggcacgc atgctatata atgtagtcag cacattggaa agcacgcctg cctcgtcccg
    28981 cagcgttacc gaaaagttga cgatctgctg tgtcatcttg tcatcatagg caaacacaca
    29041 atcccgatat ttataaaagg cactgcgtga gatccccata cgtttgcacg ccgaggaaaa
    29101 gctgcgttcg gtcttgtcgg cgaccagctt tttgacttcc agcacctttc cgaaaatttc
    29161 cggaagcact gcggtatcta ctacaatata tcgtgtccgt ttgtccatgt tcctgtctgc
    29221 acagtatcga aagcatgctt ctgtgacaaa gcctgccctc cggctgcgtt tctcctcctc
    29281 tttccgcaaa atgcaggacc ctttgccgat cccgcagcaa acagtccgtg tacaaaaaac
    29341 aattgtactt atatcaacta tataactata ccatgatttc acctgtttgt caagggcaat
    29401 ctactgtttt ttctgcgaaa acagattttt tttccttttt gatgtatccg ccgcctgtgc
    29461 cggcacccca cagaatcccg aatatgattc tttcaaaaaa agccgttttt ttcttttttt
    29521 tgataggaaa acagattttt tcattttgaa atcaaggcaa ttgcagaatc gacggaaaag
    29581 cacttgacaa agtcagaaaa ttcgagtatg ataatattgt caacggaaca cagagggatt
    29641 tcacctcatg cgtttccggc aaatgattgt gacaaggaga gccgacttga agaacagaca
    29701 gcagcaggac gaacagcata tgccccagaa acagggggat attttccgac agatgccgat
    29761 gcagagttct caaagcggtt cgaagatgat acgtgaaaca aaggcgtttc catgatgaaa
    29821 ccggtacaaa cagactgaag cttgtacaga aaacggtgaa ccatgaaaat gctttttatt
    29881 tttaggaggt aattatcatg tccaaaaatg ttgctgagta ctttgcatgc gacgtcttca
    29941 atgacgaagt catgaaggca agactcccga agccggttta taaggcaatg accaagaccc
    30001 gcaaaatggg gactccgctg gatcccacct atgcagatgt ggttgcaaat gccattaagg
    30061 actgggcgat cgagcacggt gccacacatt acacccactg gttccagccg atgaccggtt
    30121 ctactgctga aaagcacgat tcctttatca gccccactga caacggcatg gtaattatga
    30181 acttctccgg caaggaactg gtcaagggcg aaccggacgc ttcttctttc ccgtccggcg
    30241 gtctgcgtga aacctgctct gcaagaggct ataccgcatg ggatccgact tcctactgct
    30301 tcgtcaagga aggctctctg tatatcccga ccgtattcgt ttcctacact ggtgaagcac
    30361 tggacaagaa aacaccgctg ctgcgttcca tggatgctct gagccagcag gcagtccgca
    30421 ttctgcgtct gttcggcaac accaccgcaa ccaaggtcac ctctaccgta ggtccggaac
    30481 aggaatactt cctgatcgac aagaagatgt acgatcagcg tgaagacctg attatgaccg
    30541 gtcgtaccct gttcggtgca atgccgccga agggacagga actggacgac cagtatttcg
    30601 caaacctgaa gccccgcgtt gctgcttaca tggctgatct ggacgaggct ctgtggaagg
    30661 ttggcgtatt cgctaccacc aagcacaacg aagttgctcc ggcacagcac gaaatggctc
    30721 cggtattctc caccaccaac atttccaccg accagaacga actggcaatg gaagttatgg
    30781 aaaaggttgc cctgaaacac ggtctggtct gcctgctgca cgaaaaaccg ttcgcaggtg
    30841 tcaacggttc cggtaagcac aacaactggt ctatggcaac caacgacgga cagaacctgc
    30901 tggatccggg ctgcacaccg atggagaaca tgcagttcct caccttcctg tgtgcaatca
    30961 tcaagggtat tgatgaatat gcagatctgc tccgcatcag tgcttcctct gccggcaacg
    31021 accaccgtct gggtgcaaat gaggctccgc cggcaatcat ctccattttc atgggcgaag
    31081 aattacaggg cgttctggac gcactggaag caggcacttc ctacaagagc gaatccaagg
    31141 tattcgagat cggcgtagac gctctgccgg acttcccgaa ggattccacc gaccggaacc
    31201 ggacttctcc gttcgctttc accggcaacc gtttcgaatt ccgtatggtt ggttcttccg
    31261 acaacatcgg ctgcccgaac tctctgctga atgccattgt tgccggcgaa ctctctgaca
    31321 tcgccgacaa gatggaaagc gttccggctg acaagttcga tgacgagctg aaatcgctgc
    31381 tggtttccat tatcaaggaa cacaagcgta tcatcttcaa cggcaacggc tattccgacg
    31441 aatgggtcgc tgaagcagaa aagcgtggtc tgccgaactt cagatccact gttgactgtg
    31501 ttccgcacta tgcagatgcc aagaatgttg ctctgctcca gaaattcggc gtactgtccg
    31561 aaaaggaaat caaggctcgt atggaagtcc tgctggaaaa ctatgcaaag accatcaata
    31621 ttgaagccct gaccatgatc gacatgacaa agaagaagta tatcccgacc tgcattgcct
    31681 acaccaagga actgtgcgat gctgccgcag tgaagcagtc gctggacatc tgctcctgcg
    31741 tcgaaaagga actggcagaa aaggtcagca gcctgacaaa ggcagcttat gaagctaccg
    31801 agaagctgga ggctgctgta gctgctgccg ccgcaaagga tgactgcgta ctgtctatgg
    31861 caaagtccta tcgggaagat gtcattccgg taatggacga tctgcgtgcc gctgtggacg
    31921 ctctggaagt tatcacacct tctgaaaagt ggccggtacc ggcatacagc gacatcatct
    31981 tcaatgtctg atcgctcaaa tctaaagtaa ttactgcaaa gagtggttta cctcccattc
    32041 tttgagaaac cgtctgtatt cctatttgcg gaatacaggc ggttttttgc gttttgccct
    32101 tgacaattcc actgcactgt gctatactaa tattgcaaac tgttgtacga catatacaac
    32161 agttgaaaca gtcatagcaa gggagttgcg ttttctatga aatcacatcg catcaagttt
    32221 caaaaaattc tcgccgtgct tctggcgatt ctggcactgg tgggcggact gttcgggctt
    32281 tgccgtgcag tcgcctctgc acgtgcctat gatattgcac cgctgactga cgaacggctg
    32341 gcagaactgg atctgaccgg catcacccac ctgatgatcg tggcacaccc cgacgacgaa
    32401 accctctggg gcggtgctca catcgcagac ggcgggtatc tggtggtgtg cattaccaac
    32461 ggatataacg ccacccgttc cgcagagttt caggcagtca tgcaggcttc gaacaatgtg
    32521 gggctgatct tgtcctatcc ggacaaagtc gccggcaaac gggacgactg gacacatgtt
    32581 cgttcccaga tacaaacaga cctcgaaaaa gttatgacct atcagccgtg ggaggacatc
    32641 gtcacccata acgccaaggg cgaatacgga cacattcacc acaagatgac acaccagatc
    32701 gtcaccgccc tgtacgatgc caaccaattg caggaaccgc tgtacgtctt tggaaagtac
    32761 tactgtgcag taacgctgcc tgacgtacag gacagcctga caccgatctc cgatgacgca
    32821 ctgcaacaaa aggaagccct gctacagctg tacacttcgc aggcacatac cattcagaat
    32881 ctgtcccaca tgaaccccta tgagatgtgg acacagattc gcggaggaag cactgatgac
    32941 gacaacacgt aaaaagacac tgctgcactg cggaattctg acagggattc tgggggctgt
    33001 agtgctgctg atctttctgg tgcaccccgg ctgtgtatac ggggcacaga cggactggag
    33061 caaccagcac tttgccattc cggaatactt ccgcacccgt ttctatgcca ccggtaatct
    33121 ctttcccaat ttcgccatgc agctgggcag cggacagaat attttcaatt tcgcctatta
    33181 cgggctgtac agtccggtga tcctgctgtc ctttgcgttc ccgtggctgt ctatggcgac
    33241 gtacctgcaa atctccaaca tcctgctgac gctgctcagt gcatttctct gctatttctg
    33301 gctgcggaaa tggttcgttc ccaatctgtc cctttttatg gcagtgctct acctgctggc
    33361 ggcaccggtc atcttccaca gtcatcacca catcatgttc gtgaactact ttcccttttt
    33421 gttctgggca ttgttcgccg tgcatcatgc tctggagaaa cggtgcagtc tttcactgat
    33481 cgcagcatct gcctgcattc tcctcaccag cttctttttc agcgtaggag cctttttcgc
    33541 gattctcatc tacgccgttt ttctcaccct gcaccagcat catgtacacc tgttccggca
    33601 gctgatgcgg acaattggca tcacctgtct gcatttgctg ctgggagccg ttctcgccgc
    33661 gtttctgctg ctgccggtgg catggaccct gctacacggc agagatgttt ccggcagttc
    33721 tcagagcctc tggagccttc tgatgcccgg catgcacttg aattatctga catacagtcc
    33781 cttttccatt ggcatgacct ctttcagcat tctggcgatc tgtgccatgc tttgctttcc
    33841 gcaaagggca taccggtttc tcgccggtat ctttggcgtg attctggcgt gtcctctgct
    33901 gctgtacctc atgaacggca cgatgtatct ggatcccaag gcatacattc ccctgctgcc
    33961 actgctgctg ttgctgtgcg gattcttctg gaagacctta ctgtcccacc agattgccct
    34021 gcgttccacg ctgctgctgt tttccgcagt gctgggtatg ggtatcctgt cccagaccgg
    34081 aaccgatgca gagcgaattg cagtgattct ggacgggctt tcgatgctgg cggcattcct
    34141 gttctatttc cgccggcaga atgccaagcc ttccggaaac gcctccacaa aaatcctgct
    34201 gatccctacg ctggcattca gcaccgcgac ctgtctgaca gtcaatctct gtgacaccta
    34261 catgtccaag gaagatgtca gcattgtcta ttcctcggag attcaaacac tgacagatca
    34321 gattgcagca gaagacgacg gcttttaccg catttccaac gagtaccacg ccggcgacac
    34381 agtaaaccgc atctggggca gcaattacta ccggagttcc gtctattcct ctgtccacaa
    34441 tgccgcattt tccgactttt atttccggca gacccacaac gaaaacggca ttcggaacgc
    34501 cgccatgatc gtacagtcca agaatccggt ttttggcatt ctcatgggag aaaaatatct
    34561 gatcagccaa aagcccatta cccgttacgg attccggcag atcggacagg atggagcgta
    34621 tctgctgtac gagaacacac ttgcctatcc catcggcttc gccacctcca acatcatgac
    34681 taccgaggaa ctgaaaggac ttcgttatcc caatcagctg gaagcactgc tggaaaatgc
    34741 tgtagtcaat gccgcagacc tgccggagga ttccacattc cacgccaagc agccggacag
    34801 cattcatcag attgccccgt cctatactgc cacggcatac agtgacaaac agatcaccgc
    34861 cattggtgac aacaagtatt atgtgcacag caaaacggac ttctcggtaa cggtcacact
    34921 cgatgagccg atcgatgatc tgctgctgct gaaattccac gtggacaatc acctcgggaa
    34981 cggcaccacc accggcgacg tggcaattac cattaacggt atccgcaaca agctgacaga
    35041 cccccagtgg aagtaccaga acaataacaa caacttccag tacacgcttt cctccgacaa
    35101 gcccattcag aagctgaaac tgaaattttc tgccggcgat tatgtcattt ccaaaccggt
    35161 gatgtacacc atggaatatc aggtgctgga ggacacctcc gcggaaatca gcccgtggca
    35221 gctgcaccac gacaccctcg gtgatgacac catggctggc agcattcagg tgaagcagga
    35281 cggctggttc gtgctggcag tcccctatga caccggcttt cacgtcaccg tggacggaaa
    35341 ggaagccgcc tattcccgca cagacacaga ctttctggga ttcccgatca cacagggaga
    35401 gcatcagatc agcatcacct atacagctcc gctggcaaaa gccggcaagt actgctccgc
    35461 cgccggaatt tgcttgacgc tgctgttttt caccggaaac tgttggctgc ggcggcgaaa
    35521 acaaaaacaa cattcctaag gcaataccgc acaaatattt tcacaccgca tttgcagcac
    35581 atgctgcaag tgcggtgttt tatcggttca tctcccgctt gtccgtccgg ttcagaagat
    35641 agtccaccga cacctgataa aaatcggcaa tgcgtaccag cacttccgtg ggaatgtcat
    35701 gcccgccgga ctcgtaatag ctataggttc gctggctgat gaacagcacc tttgccatgt
    35761 ccgtctgtgt catgtccatg tcctcccgta agttccgaat gcgaatatac tgcataaaat
    35821 atcacccttt caagaatatg gttttatttt atgctagaac aaaacattct attgactttt
    35881 agaacaaaac atgctataat gagattgttc cattttggaa aaaagggaaa catcatcaaa
    35941 aaaggaggat attacaacat gaaacacaaa tggcttgcgg gactgctgct catcgcaatg
    36001 ctcggggcaa tgggcggctg ttccggtgcc ggaaacagca gtgcttccca gaaggcggag
    36061 gaatcttctg ccaaggaaag cagtgccgca gaggagagca gcgatgcgga agaatcccaa
    36121 aacgaggaca gcagtgatgc agaaaaaagc gacaacgctg ctgacgaatc aaaggtattg
    36181 ttcgaagaca gcactgtccg aatcacctac accggcactg acgaagaaag ctggatgggc
    36241 aaagggctga aagtcaccat tgagaatctg tccgacaaaa acatttccgt tcagaccaga
    36301 gaagcttccg tcaatggcgt catggacgat ccgtattttt cctgcgatgt ggcttccgga
    36361 aaaacggcaa atgacacgat ctatttttcc accaaggacg aactgggaac cgttgaactt
    36421 tcatttcaca ttttcgacag tgacacgttc gataccattg ccgacaccga aaaggtcacg
    36481 ctcgttattg atgactgcat caccagcgaa aaaacagaag ctgacaagat ctatgaagtc
    36541 aacggcgtag tcgtttccta tgccggcaag gtggacgacg acatctgggg cacggaattc
    36601 caatttctca tcgagaacaa cggcacggaa acatacaccg ttcgtgcgga aaacatttcc
    36661 attgacaata cgatgtacga cggttcgatg tatgccgaag tagcagccgg caaatatgcc
    36721 aacgaaacca tgtcgttcat cggcgaggag ttgccggcag aaatgaatga gttggaattc
    36781 aagctggtca tcagcaacag cagttacgaa accgtagcgg aatctgatcc gatcaaggtt
    36841 tccatacagt aatcagaaag cagaggtatt ttatcatggc aaaaggacaa aaaacgattt
    36901 acaaaaacag tgcagtcatt ctgcggctgg ttctgggcat tctctccatt gtaatttccg
    36961 cagtggtagg attccagtcc tgtgctgccg gcatcggcga agccatttcc gaagcagaaa
    37021 gttccagcgg tgctggcgga ctgttcacag gatttttcct gctggcagcc ggcattgtcg
    37081 caattgctgc cagaaagacc aagggcggca ctattgcatc gatcatctta tacgcattgg
    37141 ctgctatttt cgcctttgca aacctgagtg aaaacttcgg cgatctggtc gtatgggcag
    37201 tgctttccgt aattttcgca gtggtactgg taatcaccct gttcctgaaa gaaaaggatt
    37261 cttctgacga aaccacaaag gaataattct tctttccgca tatacaaaaa acgctgtgca
    37321 attcttcgtt gcacagcgtt tttctttttc aaacaacatc gcacaaaacc gtcaggcgta
    37381 ttttatgggt gctgcatttt cacttattct gtttcctgct gcttccggcg ggcttccagt
    37441 tcttccagag cgtctttgcg ggacaggctg atcttgccgt ctttgatgtc catgaccttt
    37501 acgataatgg catcacctac ggaaaccacg tcctctacct gttctacccg ttccttgtcc
    37561 agcttggaga tatgtaccag accgtcgcgt cccggtgcaa tttctacaaa ggcaccgaac
    37621 ggcttaatgg aaacgacctt gcccttatag atcgagccga cttccggatc aatggcaatg
    37681 gtttcgatca tctggtatgc cttgcggcag ccctccatgg aaatgccgct gataaatacg
    37741 tgaccgtcct cgttgacatc gatcttcacg ccgcactctg cggacatttt ctggatcacc
    37801 ttaccgccct gaccgatgac atcacggatc ttgtcagtcg ggatcttgat ctggagcatc
    37861 ttcggtgcga attcgcccac ggtatctctc ggagccggaa ttgccttgag catgatctcg
    37921 tccagaatgt acagacgggc tttccgtgtc ttttcaaagg cttcggcaat gattgccggt
    37981 gtcagaccgt ctaccttaat atccacctga atggcagtga tacccttctt ggtaccgcca
    38041 accttgaagt ccatatcgcc gtagaagtct tccagtccct gaatatctac catagtcttg
    38101 aactcatcgc tgttcggcag tgtgatcaga ccgcaggaaa taccggcaac cggtgcgtga
    38161 atcggcacgc ctgcgtccat caatgccagt gtcgaaccgc aaatcgatgc ctgtgaggta
    38221 gagccgttgg aggacagcac ctcggacacc agacgcatag catacgggaa ttcctccact
    38281 gtgggcagca ccggaatcaa agcacgttct gccagtgcac cgtgaccgat ctcacgccgt
    38341 cccgggccgc ggctgggctt ggtttcgcct acggagtagg acgggaaatt atactgatgc
    38401 atgtaacgct tggaggtttc ctcgtccaga ccgtccagct tctggctgtc gctgaccggt
    38461 cccagcgtag caactgtcag cacctgtgtc tgaccacggg tgaacagacc agtaccatgt
    38521 acacggggca gcacgccgac ttccgcagcc agcggacgaa tctcgtcaat gcctctgccg
    38581 tccacacgct tgccctcata gagccatgca cggacaattt tcttctgaat cagatacagg
    38641 atctcatcga tcatgccagt ttcttcgccg cagacagcat caaatgtttc gtgtacggca
    38701 tcaatgatcg gacgcagacg ggcatcacgt tcatttttat cattggtatc cagtgcgtgt
    38761 tctacctgct catgtaccat agcatcgata ttttcgaaca gttcctgatc gacctcgtgt
    38821 gcctcgaatg tgaacttttc cttgccgatc ttcgcctgaa tctcggaaat aaaggcaacc
    38881 attttcttga tttcttcgtg accagtcaga attgcctgca acatggtatc ttccggcact
    38941 tcgttcgcac cggcttcgat catgacgatc ttgtccttgg taccggcaac tgtcaggtgc
    39001 aggtcattgt gctgccgctg ttccagtgtg gggttcagca cgatctcgcc gtctaccaga
    39061 ccaacactga tgccggcgat cggtccgttc cacggaatat cggagatcga cagagcgata
    39121 gaagtggcga tcatgcccac aatatccggt gcacagtccg gatccactgc cataactgtc
    39181 atgactacag aaacgtcatt ccgcatagac ttcgggaaca gcggacgaat cggacggtct
    39241 accatacggg aagtcaggat cgccttttca gagggctttc cctcccgctt ggtgaaggag
    39301 ccgggaatct tgcctacaga atacatacgt tcttcgtagt ctaccgacag cgggaaaaag
    39361 tcgattccct ctctgggctt ggcactggct gtgacattcg ccataacaac ggtatcgccg
    39421 tagcgtaccc agcaggaacc gttggacagt gcacaggttt ttccggtttc taccgaaagg
    39481 ggtcttccgg caaatgtagt ttcaaatcgc tgtacatttt caaacataat tcgaaattcc
    39541 tttcctgatt ccaatccttc gaattcgcaa aaagccctat gctctctcct cgccttttac
    39601 aaagcacacg gcttcctgcg aatacctgaa tttggaatcg atttcataca cagaaaggca
    39661 gacaaagcca ctggctctgt gctgcctttc cttttttcaa tcgttgtttc ttacttacgg
    39721 atacccagct tctcgataac cgcacgataa cggttaacgt ccttcttctt caggtagttc
    39781 agcaggtttc ttctgtgacc aaccatcttc agcagaccac gtctggagtg gtggtcgttc
    39841 ttgtgggact tcagatgagc agtcagatcg ttgattctct tcgtcaggat tgcgatctgt
    39901 acttccggag aaccagtatc gttctcgtgg gtccggtttt tctcgatgat ttctgttttt
    39961 tcttctttca acagcatgtt ctttcacctc attcaatatt tcatcaatgc acatagaaag
    40021 aggcaggtga cttcccgacg ggacaaagca tgcacccctg cgggcttact ccaaattcca
    40081 agtacatgac aaaatatatt atatcacatc tttttcgatt tgtaaagtgc ttattttgtg
    40141 aattaagaaa taatttttcc ttttcccttg tgtgaacgtc cgccggagct tttgccgccg
    40201 gatttctttg ccggctcgat cggtttttcc tcgccgccgc ggtcctcgaa caaagtttcc
    40261 tcctcgtctg tcacggtctg tgccagttcc ggattgacct cgccccgctg ttcatagtag
    40321 ggattgatga tgtacttctg aatgaccgga taggactgga acgtaatcac cagacccagc
    40381 caggaagccg gcattaccgg cagcaggaac agcaggatca tggaataccg gatattcacg
    40441 aacatcagca ccagcacgta gaccgcaatg actgctgcca gcaccaatat ggtgatgatg
    40501 ttggttttca gggcaactac cgacagtgcc agcgaatttt tcaacacgtt tttcagggaa
    40561 agattcgtgg aaaccagcat cgggaaaatg taaaagttca tgagcagtgc aatgacaccg
    40621 atactcaggg taatggcaaa cagcacataa aacgccttgc tgccggtctg ttcgatcagg
    40681 cggggataca cgtaaaatgc cgccgagatg cagcatgcca gcacacagtc cagcacgccg
    40741 acaatacagg catgcttgaa ttctgtgcgg aacgtgtgga aaaattcatg tgccagaaac
    40801 gtaggctttt ccatgataaa atttttcagg attttcgtat gtgccgccgt tgccggaccg
    40861 atgatcactg caaacagcac cagacatgcc gccgccagaa tggttcccag ctgggaatcc
    40921 actgcaaaaa acagggcgac tgcaccgatc accggcaaaa agctgagaaa gtacagcagg
    40981 ttcagttcaa acagtttcca gaactttctg ccgaatatct caaaaaagcg gaatattcct
    41041 tttttcttgg gggcattctt tgcgataccg gaaccggcac gctcgtaatg atttccgaat
    41101 aatcccatag acaaacttcc tttcttcaag ctgctttccg tgaaaaaagc agcatacatc
    41161 ttttattccg gcagcaccgc acggcttgat gcggtgctac atttcaattg cataaaacaa
    41221 cgcactatat aacagcgtgt gcagtaccag cagcaccagc agtacggcaa aacgggcaca
    41281 gtacattcgc agctggtggc acatctgatc tgtcggttcg tcccgaaatc cataatcagt
    41341 gaattttccc gagaaccgca gggactcccg cgatgccacc gccagcagtg ccgaaaccgg
    41401 cacaaaataa atcagcagga gcacactccg caccagcaca tttccggagc cgcactgcat
    41461 ttgcaaggta ccgcccatgc aaatgccgta agtcagcagc agcagccata caaaaggctg
    41521 tcccaccgcg gaaaacccca gcaggaaaaa ggtcatcaga aacagcaaaa tgttcccgat
    41581 gctccccagc cagagggctg ccagactaga ccgtttttcc agaaacatgc ccagaaaaca
    41641 cgcccacgag gtgggaacgc cgtcatactg ccaccgcagc atgacaatcc ccagtgccgc
    41701 tcccagcaaa acgctgccga acagcaggca gaacagccgt tgttttcgct ccgttgttgt
    41761 caatgtcgtt cgctttcgga tcattgcaga tctctccttt gctttgcaaa ttggcttgta
    41821 cctgctttaa tcctatgcga aagacacacc ggttatgaac gccgcgactt tatgcagtgt
    41881 ttcctttact tagacagttc gtatgcacgc ttggtgcagc tgtcgcaggc gttctgcact
    41941 gcgtccagca ttttgccctc atacagctga tccagaccgg caagcgttgt gccgcccgga
    42001 gaggacacct gacggatcag ctcgtcaatg gagtaaccgg aatcggtaat catctttgcc
    42061 gagccgatca gcgactgcac aaacagttcc ttggcagact gttccggcag tcccactctg
    42121 gcagcataat ccagaaagcc cttggcgaac agatagataa acgccggact gctgccgtta
    42181 atggcaatga cctctttcat ctgctccggt gcaatgaccg cagtcatgcc gcagctgccg
    42241 aagatattcc gcaccactgc aaattctgca tcactgacgg aatcgtcttt tgccagtgcc
    42301 gtggcaccct ctcccaacag cagcggggtg ttgggcattg ccagaactac ctttgcaaaa
    42361 ggctttgtca gcttccggat aaactccgca ccgattccgg cagcaatgga caccagaact
    42421 gtctgttccg ttacagcatc cgcgatctgc ggcagcactt cctcgaattt ctgaggcttg
    42481 atcgccagaa aaacataccg gctcttttcc accacttctg cggcactttt acacttctgc
    42541 acaccgcagt ctgccagagc gtccactttt gcttccgtgg ggttataggc gaacagttcc
    42601 gcttccggct tcatatcgct tccggcaatg ccccgcaaaa tggcactgcc catattgccg
    42661 gcaccaataa aaccaatggt attcatgaat ctttccctct ttcaattttg ccgttttttc
    42721 gaaaatacga catttttcat ggatatatta tactgcaaaa caataaaaaa tgcaagtcct
    42781 cagcgtatat ttctagggaa acactgcata aagcttgcaa gcagattgcg tgcaaagccg
    42841 tcaggcgaac tatgcagtgt tttcctataa aagaaaaagt ggacgcactc tcgcacgccc
    42901 acccctcttt tatcaagcgt ctacacgccg atcaaaatgc aatatcttct gcgatctggt
    42961 gcagttttcc atcatacggc ttttccatag acagagcttc ctgaatgtca aagtctacga
    43021 tcttgccgcc ctgcataccg acaacacggt taccgatgcc ctgttccagc agttctacag
    43081 catagtagcc catttctgtt gctgctacac ggtcgcggac tgtcggagaa ccaccacgct
    43141 gtacgtggcc caaaatggtc gcacgggatt cgataccagt cttttcctgg atcagcttgc
    43201 agatctcttc ggaatgaccg atgttttctg caacaacgat aatgaaattc tgcttgcctc
    43261 ttgccttgaa ttccatcatc ttcttcgcga tcttgtccag atcatagggc ttttccttag
    43321 tgatgatctc cagtgcaccg gaagccacgc cggtattcag tgcaatgtaa ccagcaccac
    43381 gacccataac ctcaacaacg ctgcagcgat agtgagactg gctggtatcc gacagcttgt
    43441 ccatcagttc cattgcagtg ttcattgcgg tatcataacc aatggtgtaa tctgtgcagg
    43501 caatatcgtt gtcaatggta cccggcagac ctacacacag aatgccgtgt gcagacagat
    43561 ctgcggcacc gcggaacgaa ccgtcaccgc cgataacgac cagaccttcc agaccgtact
    43621 tttcacaggt agcctttgct ttcagaacac cctgttcggt gcggaactct gcacttcttg
    43681 cggtgtacag catagtaccg ccgcggctga tgattcctgc tacactcatt tcgtccatcg
    43741 gctcaatatc gtcttccagc agaccacagt agccacgctt tacgccatat actgtcatgc
    43801 cctttttcag agctgcacgg acaacggcac gtaccgctgc attcatgccc ggggcatcgc
    43861 cgccgcttgt caatacacca atcgttttaa tcataaacgt attctccatt ccgccgcatg
    43921 gcaaaatttt gaaacggctg cacagcacat tcgctgctta tgctttcacc attccattcc
    43981 tattctatta tactgatatt tcagaggttg tcaagtggta ttcgaaaaag ttaacaagtc
    44041 ccatatatat tttatcaaaa aatcacaaca gctgtattca gtgtttcctt tactgccgaa
    44101 cctgttgcag ttacgccggc ggcagaaatc ccacctgctc cggcgaaacc acggcacaga
    44161 gtgcggcata ggattccgaa ttcaccgcca ccgcctgtcc gccgcgggga gagagcatct
    44221 tcttccggtc tgtcaggtaa aagcagaccg gcacatttcc cgcatgctgt ccgatcactg
    44281 ccagcagctg ctgtaattcc gccgtctggc gggaatcgat cttcacgcag agccgcccct
    44341 gcgacaacgt ccggaaaaag gcttcttcct gcaatatgct gtcacacagc acactgacgg
    44401 cgttttcttt ctgggaaatt ttcccgcgga gatacagcac cgcgtcctgc tgcaagtacg
    44461 gctttacaga attgtacagg gcaggaaata ccacgcagtc cgccgaaccg ctttggtctt
    44521 cacaggtgag aaaacacatg gtttcccctt ttttggtggt gtgctgcttg acatgctgta
    44581 gcgtaacgat cagcagtact ttcccgccgt ccggctgttc cgacagtgca gccgccggcg
    44641 tggcacggca aagctgccgc agccactggg actgttccag cgggtgaccg gagagataca
    44701 gccccagact ttcatgttcc agctgcaata gtgcagagcg ttccagttcc ggcagcgacg
    44761 gaatcggcgt ttcccgcacg gcttcgctct cgccgccgaa cagattcatc tgtccggtca
    44821 caatgctgct gcgttcctcg cggcagtcca tcagcacctg ttcctgatac tccagcatct
    44881 gccggcggtt cagtcccaga ccgtccagag caccgcagcg aatcaggctg tccagtgcct
    44941 gtttgttcag tcccagcggc agcatacgcc ggcagaaatc tgccagaccg gtgaattttc
    45001 cggctttctg ccgctgctcc gtcatcttct ggatcaaccc ctgccccagc ccttttactg
    45061 ccagcagtcc gaaccggata tgctcaccgc agcgaagaaa atctgctgtg ctctcgttca
    45121 catgaggcgg cagcaccttt acgccggcgt tctgacaggc ggtgaggtat tccatggttt
    45181 tccccgtatc acccatgaca ctggtgagca gtgccgccat atcgtctgcg aaatagtgca
    45241 ctttcagata ggcagtctgg tacgccagat aggcatagga aaccgcatgt gctttattaa
    45301 aggcgtaatc cgcaaacaca gcgatctcat cgaaaatcgc gttggcagtt tccaccggta
    45361 cgccgtttgc cactgccccg cagcagggag aactgccgtc ggagccgtcg ctgccgtaaa
    45421 tgaatgcctg ccgttcccgt gccatgacat cctgcttttt cttcgccata gcacggcgga
    45481 caatgtcggc tctgccatag gaatagccgg acagcacccg acagatctcc atgacctgtt
    45541 cctgatagac catgcagccg tatgtgggac gcaggatcgg ttccagcagc gggtgggcat
    45601 agacgatctt ctccggattc cgccggtttt ccagatagag gtcaatggac gaacgcggtc
    45661 cgggacgata cagggacagg gcaatggtca ggtcctccag acaggtgggc ttcagccgca
    45721 tcagcacgtg ccgcataccg gcactttcga actgaaacac gccggtggta tctccgcggg
    45781 aaagcatggc ataaactgcc gcatcgtcca gcgggatcgc atctatggag aatgctggtt
    45841 catacttctg aacggcacgg acacagtccc agataactgt caggttccgc agccccagaa
    45901 agtcaaattt cagcaagccc atagtttcca gaacgcccat gggatactgg gtgatgacgg
    45961 tttcctcatt tcgctgcagc ggcaccaaat cagtgatcgg caccgcagag atcaacacgc
    46021 cggcagcatg ggtagaagcg tgccgcggca tgccctccac ctgcattgcc atatcgatca
    46081 gcttttttgc tgtagaatcg gtttcataca gtttccgcag gtcttccccg tccttgccct
    46141 ccatggattc cgcaatgctc ttgcgagaat cgatagactt tgcaattttg tcgcacagcc
    46201 cgtaggaaat ccccattgcc cgtccggtat cccgaatggc tccccgtgcc ttcatcaggt
    46261 cgaaagtgat gatttccgac acccgatcgg caccatagcg ggagatcaca tactccttga
    46321 cacgctgtct gccctctacg cagaagtcaa tgtcaaaatc cggcatgctg acacgctccg
    46381 gattcaggaa gcgttcaaac agcagctggt atcgcagcgg gtcgatctgg gtaatgccca
    46441 tgcagtaggc acacagactt cccgcaccgg aaccacgccc cggtccaacg ggaatgtcgt
    46501 gacttctggc gaaattgata tagtcccaca cgatcaggaa gtaatccacg tatcccattt
    46561 gggtaatcac agagatctcc atacgcagcc gttccagcac ctccggtgac gggtgctctc
    46621 cgtagtgctt cttcaagccg ttcatgcaaa gctgtaggaa cagcttccgg cagtccgcca
    46681 cgccctccat ggaaaatttc gggagataga tctgtccctc ctgaaattcc acgtgacatc
    46741 ttgccgcaat ttttgctgtg ttggtcactg cctccggcac atcggcaaac agtgccgcca
    46801 tttctgccgt agatttcaga taaaactccg gcgtttcaaa gtgcatatgc tccggatcgt
    46861 ccagcgtttt gccggtctgg atacacagca gcacttcctg catggcagcg ttttcccgtc
    46921 ggagatagtg ggcatcgttg gtggcagcca gcgggattcc cgtttctctg gaaagccgca
    46981 gcagcagggg caggttttgc agttcctccc gaatctggtg gttctggatc tccagatagt
    47041 agttgccctt gccgaacaac tgctgatacc gcagggcagt cgcctttgcc gcggcatagt
    47101 cccctgccga cagcagacgg ggcacttctc cagccagaca gccggacaga caaattaaac
    47161 cctcatgata gcgggagagc aattcccaat ccacacgggg cttttgatag aagccttcca
    47221 gactggcaag cgaaaccagc ttgaccagat tctgatagcc cttttggttt tcacagagca
    47281 ggatcaggtg gtgacttctg ctgtccagcc ggtgctcccg atcgtgacgg gtacgccttg
    47341 ccacatagac ctcacagccc agaatcggct ggattccggc ttttttggca gcctgataaa
    47401 acggcatcac gccatacatc acgccgtggt cagtcaaagc gaccgcagtt tgtcccattg
    47461 ccctaacctg ttcgatcagt tccgggatcc gacaggcacc gtccagcagg ctgtattcgc
    47521 tgtggacgtg gagatgtaca aaagcatttt caggcatctg cggcacgttt tctcaactcc
    47581 tctgcaaaag cggcgatttt ctgaaagcgg tggttgacac cggaacgccc gataggttca
    47641 gacagcagtt ctcccagttc tttcagacta agttccggat tttccagccg cagttctgcc
    47701 agttcctgta gttccggaga aagtgcctcc agctgcccgc actgggtgat ccactgaatg
    47761 tcctgtatct gccgtatggc ggcaccgacg accttgttga tattggcctc gtcgcagttc
    47821 cgcacacggt tcgccttgtt ccgcatatcc ttgcggatct tgatattcat cagggcaatg
    47881 gtgcactgct gggcacccat ataggtgagg gtgtcctcga tgtgctcgct gtctttcagg
    47941 tacagcacgt acatgttttt ccgcagcagc tgaccgccgc tgatgccgat gctccccagc
    48001 agcttttgca agtcggagca gagcagttcc gacggcgtgt tgaattccag atgatattcc
    48061 ttgttggggt cgatcatgct gccgcagccg aaaaatacgc ctgccagaaa agcactcagg
    48121 ctgttgttca ccagattgga aagacggatc tcccgctttt ccggacgaat gtgatagact
    48181 tcacaaatgc ggctgatctg tgccgcatcg gtaatggtat agctgaccat ggactggtcg
    48241 ccgccgcggg agatcgtccg cacatcgggc tgcagttctg tccggaacac cgcacggaac
    48301 agcatgggca tcagccgtgc gaacaccgga ctttcgcttt gcagcaccac ttgttcttgg
    48361 gtaaaaatgc gggaatacag caaaatgccg tacagacagg caaactgccg gtcgcggtcg
    48421 gtgatgcttc tggcgatctc ttcccgcaca tcattggaaa atgacacggt ttccttttct
    48481 ccttatggtc ggtcctttcc aatatcgcga tgctgtttca caacatgcat gcccttggaa
    48541 atgagatagt cccctatcag ttcagtaaat gtgatcgaac ggtgcttgcc gccggtgcag
    48601 ccaaatgcga tcaccagacg gctcttgccc tcctgaatgt acagcgggat caaaaaatcc
    48661 agcagatctt tgagtttttc cagcagcgtg cgggactgct caaaggacat gacatattcc
    48721 tgcacacact tttcacagcc ggtatgatgc cgcagttccg gaatgtagta cggattcggc
    48781 aggcaacgca catcaaacac cagatcggat tccgtagaaa cgccgtactt gaacccgaag
    48841 gaagtcacct taatggacat ggagtcagag gaattgtcca gaaagatctc ccgaatctgt
    48901 tctttcagct gcgaagccga gaaataggac gtttcaatat aataatctgc aatgcccttc
    48961 acacggagca gctgctcccg ttcgtaggca atggcattgt gcaggcagcc cccgaaccgt
    49021 tcgtccagcg ggtgcttccg gcgggtttcc ttataccgct tgatgatgac ttcgtcctcg
    49081 gcttcgatat agaggatctt cacgtccaga tctgcctgct gcttcatctc cagcagtgtc
    49141 cgataggcat ctgtgaacat atcgccggaa cgcaggtctg cggcaattgc cagtttttgc
    49201 aggtggtttt ctgactccct gcaaatctcc acaaatctcc cgatcagctg cggcggcaga
    49261 ttatcgatac aataataccc gatgtcctcc agaacgtcca ttaccacaga ctttcccgaa
    49321 ccggacattc cggtcactac caccacatgc attctcacat tttcctgttc ctgcatatgc
    49381 tccacgtcct tcctacacac ttttcaacaa tttccttagg caataccgca caaagagcgt
    49441 ctggcgactt tgcacgccat ctgcttgc
//
LOCUS       DAWBGR010000002        39252 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_433_length_39252_cov_3.363395, whole genome shotgun sequence.
ACCESSION   DAWBGR010000002 DAWBGR010000000
VERSION     DAWBGR010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 39252)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 39252)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..39252
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_433_length_39252_cov_3.363395"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    23383..23392
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    24744..24753
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9004 a        10783 c         9233 g        10212 t
ORIGIN      
        1 cagcaggccg ggcgcggctg cgctacaaat tcctgaagcc cgcggaccgc agcgagtaca
       61 aggccatgct ggcggacggg caggcggatt ttgtcattga cctgaccgac gacatgtccc
      121 aggcggagaa ctacggctac aagctgaccg acagctatct gtcggcggag ttttcctggg
      181 tgctgctgcg ccgccacaac ggcgcgctga acaaagtcgc tgtcgcatac gatttttccc
      241 cctccgcgct ggaaatgccc ggcctgagcg acacggctca tgtagaacac ctagattcct
      301 ttgatgactg cctggctgcc gtgcgcaccg ggaccgtcga tgcctattat acctacacct
      361 accaggccga gcgcacggtc tttgacgaca cgcgcaacga gctgcgctcg atgctgagcg
      421 gcgagcagcg caatttctgc atcggcgtcc ggcaggacta cgatgtgctg ctgcgcactg
      481 tgctgaacaa gagcatcgac agcctgaccc gcgccgagat cagcaccatc accaacgcct
      541 acgtcaacct ggggcagcag ccgttctccc tgacgcggct ggcctatcag tacccggcca
      601 ttatcgtgct gacctgcctg tgcgttctgg ttaccgcggt ctgcatcgca ctggtcatcc
      661 gcgcccaacg gttccgcgcc gagactgaaa aagccctgcg caaggccgag gaggccagcg
      721 tcgccaagac ggagttcctg tccaacatgt cccacgacat ccgcacgccc atcaacggca
      781 ttatgggtat gctggacatt gccgaggaca actttgacaa caaggcccgc gtgcgggact
      841 gcatgaccaa gatgcgcggt gcggcgtccc acctgctctc cctcaccaac gacgtgctgg
      901 acatgaccaa aatcgaaagc agacccattc gggacggcag cttcgacgtt gtttttcaca
      961 tcgacttcaa gggcaatctc cgcgaccgga gcattgccgc atttttgaat gatctctgca
     1021 aaagctgtcg ggatttcaag ctgttgggaa atgctgtcgt catggtataa tctgatctat
     1081 tgcaaaaaca gtttcaaaag cagtccgttt gccgcggact gctttttgtc attcctgttc
     1141 tccacgcatc agttttgaac gaaattccgc ctcccgaaac tgcttcggcg taacaccgac
     1201 ttttctggaa aagacctgta tgaaatggct ctgggaagaa aacgccagca ggtttgcaat
     1261 ttcagcgtag ctgcggtcgg aaaattgcag catgcgtttg gcagactgaa tcttttgatc
     1321 taaaatataa tcatgcagcg tacagcccat ttgctcccga aacagacggg ataagtaact
     1381 ggggtgcagg tgcacttccg cagcaagttc ctctgtggaa attttttcgt gcatatgtga
     1441 atagatgtag ttcaggcaca gtgaaaccgg cttggaacag acctgcttga ttttccgctg
     1501 ctgcattcgc ttgacatagt ccatacaagc gtcatcatgc agtttctcca cctgatcgat
     1561 ggtcgttgcc gtgtcaattt tacgaatata gaaatcgctc atacgatatg cctgttcccc
     1621 cggcatgccg ccttcaatac agaaccgcga taccattgac agacagacaa tacagtgata
     1681 ccggaaattc tgcaccaaat cgtcagaaag ccttactctc atggcgatct gccgatatga
     1741 tccggatcgc agattttcct gcactgcatc cacatcgccc tgctgcactt gattataaaa
     1801 gccaaattcg acttccggcg gctcgtgctg gtgcgtatcc tcctgccgtt cctgctctgc
     1861 tttttgccat tctaactcca atgtcatgaa aatctcctcc tactatttta tatcgcctta
     1921 aatgtgaata aatatagaat tcctttcaga aacaaatcta taacaaaatc ctgctctatc
     1981 cgttctcttc ggcaacctgc actgaaaaaa gtactgtatt ttccagaaca actggaaaat
     2041 acacatcgac ctctgctggt tttttacgga aacatgggaa aaataagaaa tccctataat
     2101 tactctatga tttcagggcc caaaaagttg catttatcct gtacagaaat attgtatcac
     2161 atttctatca aaaaagcaag ttttttggta tcattttttt gatattcctc gtaaaatgaa
     2221 aatgcagata tatgaaaggg gcattggctc tgggattatt tggtgtccag cagtattccc
     2281 cacggctctc gttttcacgt gtccgcttca taagcatagc ggattcaaag caattcaaat
     2341 agaaaattgc ttccgtaaca gacaacagcc actgctattt cgattgacag gaggtcacat
     2401 catgcgtatt tggaaaggct atcagcatgg tatcaatttt ggcggctggt tttcacagaa
     2461 agagcatacc gaaaagcatt atcagacatt tatcacggaa gcggactttg caacagttgc
     2521 ccgctgggga ctggatcacg tccggattcc ggtggactat gaattggtag aatccgaggg
     2581 tggcattctc aaggaagacg gttttcgctg gatcaagcag tgcattctct ggtgcagaaa
     2641 atacgatctg cacatgattt tgaatttaca caaaactgcc ggctattctt ttgccgatga
     2701 gtcagattcc ggtgaattct tcaccaatct ggcattgcag gctcgcttta tctctctttg
     2761 gcaggaattt gcaaaacgct ttggaaatga cagcgatgtt gtcgcctttg aactgctgaa
     2821 tgaagcggca gacatgcaca ccgcaaaaga ctggaaccag atcgctgcca acgccattgc
     2881 agcgatccgc tctgttgcac cggatgtttt tattctggca ggcggcattc actacaacgg
     2941 tgccgacggt gtgacactgc tggatgcacc ggcagattcc cgcatcgtct acagtttcca
     3001 ctgctatgat ccgatcattt tcacgcatca gactgcacag tggatcaagg gtatgccgaa
     3061 ggacttccac attgcatatc cggacactct cgccaactat cgccgctggt ccgctgcatt
     3121 cagcaaggaa tatatcggtt cacttctgca tgaagggctc acagatgtgg aagcttcttt
     3181 ctttgaagca aatctttctg cggcaatttc tgctgcggaa aaatatgatg tgcctctgta
     3241 ttgcagcgag tacggtgtca ttcatcaggc agatctggaa agcactctgc attggtatca
     3301 gactatttcc actgtatttg aaaagcacca catcggacgg gctgcctgga catataaaga
     3361 aatggacttc ggtctgacag atgcggaagc attccccgtg atgcagaaag tcctgccttt
     3421 gctgtaatct atatggcaat accgcacaaa gattgtacgg tattgccata gaaataaatt
     3481 atgttttgat gcccctgctg ctttttacgg ctggggcatt tttgctgcaa atttgcccta
     3541 acaatcttgt ctttacaaac gataaatttc atggtataat agaagaagaa gacttgcaac
     3601 aactgcaacg gaggtccata tggtaacaaa tgatggtacg atcattcaga tcaaacatga
     3661 tattctgtgc gaagtcgcaa aattggtttt cgccggaaaa tttgaaacag aaaaagacga
     3721 gctgccctat cgcatgatgc ccggtccgaa agcaaaatac cgctgctgtg tctacaaaga
     3781 acgggaaatt gtccggcagc gaatccgcct tgcagagggg aaggctccgc agggatcaca
     3841 caacgacctt gtggtacaag tcgtacaggc ggcatgtgag gactgcccta tttcccgcta
     3901 cgttgtcaca gacaactgcc agaagtgcat ggggaaagcc tgtcagcagt cctgtcattt
     3961 cggagcaatc gatattggcc gtacgcgggc acacattgac ccgcagaaat gtcgggaatg
     4021 cggcaagtgt gcggcagcat gtccctacaa cgccattgca gacctgattc gcccatgtcg
     4081 ccggagctgt ccggtaggag cgatttctat ggacgaaaac ggcatctgca tcattgacga
     4141 gaaaaaatgt atccagtgcg gcagatgtat ccacagctgc ccgttcggtg cgatcagttc
     4201 taagtcctgc ttggtagatg tctgtcaggc actagtggac ggcaaacatg tctatgccat
     4261 gtttgctccc tctacagaag gacagttcgg cgaaggcatt actttcgaaa gctggcggaa
     4321 cactctgaag gaagtgggct ttaccgatct ggtagaagtc ggactgggcg gtgatatgac
     4381 cgcttatgcc gaagcagcgg agtgggcaga agcatatcgg gagggcaaaa agctgacaac
     4441 ctcctgctgc cccggctttg tcaatctgat tcgtctgcac tatccggatc tgatcgacaa
     4501 tatctctact accgtttccc ccatgtgtgc ggtttcccgc tggatcaagt ccaaggatcc
     4561 ggaagcggta actgtttttg taggtccctg catcgccaaa aagagcgaag tcatcgacca
     4621 gcacattgag ggcagtgcgg actacgccat gacctacggc gagatccgtg ccattatccg
     4681 tgccaagggc gttaccattc acgaagcaga ggaaaccaat cagcagtcca gcactttcgg
     4741 caaatggttc ggcaacagcg gcggtgtgac agatgccgta ttggaaggca tgaaagaaca
     4801 ggacgtgacc gacgaagtca atgtggaacg ctgcaacggc actgatgcct gcaagcgtgc
     4861 cctgctgatg ctgaaaatgg gcagattctc cggcgacttt atcgagggca tggcttgcga
     4921 cggcggctgt gtaggcggcc ccagccgcca cggtgaagtg aacaagttca aacgtgccag
     4981 agaaaacatg atcaaaaagg cagacaaccg cagcattcat gagaatctgg aaaagctggg
     5041 agcagaacaa attcccatgc accgcgaaga atctctataa ggaaaagcac tgaactgcat
     5101 atagcataca taaaaaaatc cccgaaaacc ggaacaaatc cggtttcggg gattttgtat
     5161 tttttattcg gcatccgcct gattcttccg gtatgccaga tagctttcca gaatgatcgt
     5221 tgccgcaaca gcatctacca cggctttccg ctttttgcct ctggtattgg tgacattcag
     5281 gtaggaatgt gctgaaactg tggtacagcg ttcatcccac atcaccaccg gcagaccggt
     5341 cagttctttc agaccgtccc cgaacttctg acacagttcc gcacgttctc ccacggtacc
     5401 attcatattc ttgggatagc ctaccaccag cagttctgca cggtgttcct tagcagcggc
     5461 agcgactttt tcctgacacc gggtaaagtc cttttccgaa atcaccccag ctggagaagc
     5521 cagcatttcg cccttatcgc aaacggcaag ccctgtacgg gcatcgccga aatctacgcc
     5581 cataataatc ataaaacgcc tcacttacca cggctgcacg gtgccgataa tttcctgcac
     5641 cgaagcaata tgatgtgcgt ccagccagtc gttcatctcc gaaatgatgc gaactggagc
     5701 ataaggatcg gtgaaaatag cagctcctat ctgcacggca ctggcacctg ccatcatcat
     5761 ttcaatggca tctgtaccgc tggacacgcc gcccataccg attaccggaa tggaaactgc
     5821 ctgtgcgacc tgccatacca tacgtacagc aatgggaaaa accgcagggc cagacatgcc
     5881 gccgacgttg ttccgcagaa tcgggcggcg attttcgata ttgatccgca tgcccaaaag
     5941 ggtattgatg agggaaacgc tgtcggcacc ggcagattcc actgccttgg caatatcggt
     6001 aatgctggtg acattgggag aaagctttac gatcagcggc ttcttggttg cctttcgcac
     6061 tgctgcggta atcatttccg cactctcaca gtgcacgcca aacgctgccc cgcccttttt
     6121 cacgttaggg caggagatgt tcagttcgat catatgcaca tcggtttcgt ccagcttttc
     6181 cgcaatggaa acgcattcct ctacggtaga accggcaata tttgccagaa ttatagtatc
     6241 ctttgtcagg agattcggca gttcccgttc cagaaacgcg tcgattcccg gattctgcaa
     6301 ccctaccgag ttcagcatac cgctgggagt ttcggcgata cggggagccg gattgcccgg
     6361 acggggctgt ccggttgtac ccttggtggc aatgccgccc agttccgaaa gcgggaacag
     6421 tttctcgtac tctctgccgt agccaaacac gcctgatgcc ggaatgatcg gatttttaaa
     6481 ggatacgccg gctacattta ctgctaaatt tgccattacc aaacaacctc ctctgccgga
     6541 aataccggac cgtctttgca gacgtgtccc atcacggatt tgccttcccg ttccagctgg
     6601 catgcacata ccagacaggc accaatgccg cagcccatgc gttcttccat ggaaacctca
     6661 cagcgaacgc cgtactcctt tgcggcggca gccacgaatt tcagcatgag gcgtgggcca
     6721 caggcatata ccatgtccac gtcacccttt gcaagctgtt ctttcagcgg ctctgtaacc
     6781 aaatcctgtc tgccggcagt gccgtcatcg gtacagagaa tggtttccgc accagatgcg
     6841 tggaattcgt cctgcaatgt gacaatgttt gccgaacgga aaccacatat tgctgtggca
     6901 cgttcgccat acacctgtgc cagcgacagc atgggcggat tgccgatacc gccgccaacg
     6961 actaccacat gttttgcctc cggcagaatg gtgaagccat gtcccagcgg tccgagaatg
     7021 tccacgtttt cccctacccg cacattggcg agagcatcgg taccagtgcc ttttacctca
     7081 aacacaatgg tcagggttcc ggcagctttg ttaatctcac agatcgagat cgggcgacgc
     7141 agcgtgcagc ctacaggcag caggtgcacg aactgtccgc acgctgcctg ctgtgcaatg
     7201 gaacggcatt gcagcaccat gcgataaata tttttagcaa gtgcctgctt ttccagaacc
     7261 ggcaccagca tctgttcata tgccatgaca atttctcctt tcaacagcaa aaagggcggg
     7321 cgggaaatct ccccgtccgc cacggtttgc gtgattttta aagtcagatc tttgtaatat
     7381 cgaccatttc tacatcatca atgctgcggc cggactccag tacgtttgcc agagccttgg
     7441 ctgtatcgat cgcagtcaga cagcagatcg aacgttctac ggacttccga cgcatcttta
     7501 cgctatctct tgccggcaga cgaccttttg ccgaagtgga gatcatatag tggatcttgc
     7561 cgctttccag cagtgtaatg gtattcggtt ctccgtcaga gattttcttt accagattgg
     7621 tggcgatcat gttatggttc agaacggctg cggtaccgct tgtaccgtac agttcgaagc
     7681 ccatacgtgc cagcttgtct gcaatctcaa tgatttcctg tttgtcgctg tcgcggacag
     7741 aaatcagcac gccgccttcc ttcttcaagt catagcctgc ggcgatcagc cccttgagca
     7801 gtgcatcttc cagattggtg gcaataccca gacactcacc tgtggacttc atttccggtc
     7861 cgagcatggt gtctacgtcc tgtagctttt cgaagctgaa taccggtacc ttgacggcaa
     7921 cgtacttgcc cttcggatac agaccggact tgtagcccag actctggaga gtttcaccca
     7981 gcatgatctt ggttgcgatg tctaccatgg ggataccagt taccttactg atatacggca
     8041 cggtacggga cgaacgcggg tttacttcaa ttacataaac ttcgttattg tataccacgt
     8101 actgaatgtt gatcagacca actgcgtgga gttcctttgc cagcagtccg gtataacgca
     8161 cgatggtatc agtaatcttt tcgctgaggt tctgtgccgg ataaatggag atcgagtcac
     8221 cggagtggac accggcacgc tctacgtgct ccatgatacc cggaatcagg aagtccttgc
     8281 cgtcgcagat cgcatcaact tcgacttctg tacccatcat atatttatcg atcagcaccg
     8341 gattttcaat catatggctg gtgatgatcg ccatatattc ttcaatatcc ttgtccgaat
     8401 gtgcgataat catattctga ccgcccagta cataagacgg acgcatcaga accggataac
     8461 ccagatcgtt ggcagccttg agggcttcct cagtcgtcat gacggtttca ccagccggac
     8521 gcggaatgcc gcatctgccc agcagttcgt caaaacgctc tctgtcttcg gctgcgtcaa
     8581 tatcgtctgc ggaagtaccc agaatattaa cacccatatc tgccagatac cgtgtcagct
     8641 taatggcagt ctgtccgccg aactgtacca caacgccgta aggcttttcg gtgttgatga
     8701 tattggcaac gtcttccttg gtcagcgggt caaaatacag acggtcgcca gtgtcgaagt
     8761 cggtggaaac ggtttccgga ttgttgttgc agatgatcgc ttcacagccc atttctttca
     8821 gagtccatac gcagtgtaca gaacagtagt cgaactcgat accctgaccg atacggatcg
     8881 gaccggaacc gaatacgata atctttttct tgtcgctgtg ctggcgttca atgaattctt
     8941 ccgcttcgtt ctcgtcatca taggtggagt agaaatacgg cgtttctgcc ttgaattcgc
     9001 ctgcacaggt atctaccatt ttatatacag catggcggtg catcgggcac ttcttgccgg
     9061 aaatacgttc aatggtcgcg tccagatagc cgtactgctt tgccagcagg tacagatctt
     9121 cggtcagcgg cttggcagcc atttcccgtt ccagttcgat taggttcatc agcttgttca
     9181 ggaaccagca gtctaccatg gtcagttcgt gcagttcttc taccgtcttg ccacgcttca
     9241 ttgcctcata cagctggaat gcacgctcat cgtctacgac ctgcagccgt tccatgattt
     9301 cttccagagg catcttttca tacttcttca tgttcatgga atctacgccc agttccgtcg
     9361 aacgaacagc cttcataatg gcttcttcga agctggtacc gattgccatg acttcaccgg
     9421 tcgctttcat ctgggtgccc agtgtccgct ttgcgtagac aaacttatca aatgcccact
     9481 tcggatactt gacaacgacg tagtccagag ccggctcgaa gcaggcgtag gtcttgccgg
     9541 taacctgatt gacaatttcg tccagtgtgt agccgatcgc aattcttgcg gcaacctttg
     9601 cgatcgggta accggtcgcc ttggaagcca gtgcagaaga acgggataca cgggggttta
     9661 cttcaatgac tgcatattcc atgctggtgg ggtgcagtgc aaactgacag ttacagccgc
     9721 cctctacctt cagttcggaa ataatgttga tcgcagcggt acgcagcatc tggtattccc
     9781 gatctgtcag agttacagtc ggggcgataa cgatactatc gccggtgtgt acgccgaccg
     9841 gatcgaagtt ttccatggag cagacggtga tgacgttgcc cttgcggtca cggatcacct
     9901 caaactcgat ttctttccag ccgctgatgc acttttcgat cagaacctgt gtgatcggag
     9961 aaagacgcag accgttcttg gtgatgtcgc ggagcatttc ttcattgtct gcgataccgc
    10021 cgcctgtgcc gcccagtgtg aatgccggac gcacgataac cggatagccg atggtcttgg
    10081 caaaatctac ggcatcttca atggtttcta cgacctttga cgggatacag ggctggttga
    10141 tcttttccat ggtatccttg aatgcctgac ggtcttctgc cttatgaatg gtcagcgggt
    10201 cggcacccaa cagcttgaca ccgttagctt ccagaaagcc ttcttccgcc agctgcatgg
    10261 acaaggtcag accagtctga ccacccatgg tggacagcag gctgtccggc ttttcgatcc
    10321 gaatgatccg ctttaccacg tccagcgtca gcggttcgat atagacctta tccgccatcg
    10381 ccttatctgt catgatcgtt gccggattgg agttgatgag gacaacttcc agaccttcct
    10441 gtttcagtgc acggcaagcc tgtgttcctg catagtcgaa ctccgcagcc tgtccgatga
    10501 tgatcggacc ggagccgatc acgagaacct ttttaatatc acttctcaat ggcataactt
    10561 acttcttctc cttttccatc acagcaacga attcgtcaaa caaatatgcg gtatccagcg
    10621 gtccgccgtg tgcttccgga tggaactgca ccgtaaaggc attgatcttg tgataacgaa
    10681 ttccctcaca ggttttatca ttggcgttga tatgagaaac ggttcccact tccggatcca
    10741 gactttctcc tacaactgca taaccgtggt tctggctggt gacataagtc cggtgactgt
    10801 tcaggtcgat aaccggctgg ttggcaccgc ggtgaccgta cttcagcttt tctgtccgac
    10861 atccctgcga cagtgccagc agctggtgtc ccagacagat gccgaagatc ggaatgccca
    10921 gctttgcaat ctcgcccaca ttgtcgatga tcgcctgatt ttcggacgga tcccccggtc
    10981 cattggaaag catgatgccg tctacgtgca tcgcctttaa ttcttctgca ctggtggctg
    11041 ccggcagaac tacgacttca cagccgcgtt ttaccagttc gttccggata ttgcgtttgt
    11101 aaccgaaatc gtacagagcg accttatact tcggtgttcc ctcacgataa gttctcggct
    11161 cgtcgccggt cacgctgaga attgcattct gaatggtgta gttccgaacc tgttccagca
    11221 gctcttcttt cttggcgtaa acatcttctg ttgtaatcac gccgttcata acgccgactt
    11281 cccgaatgat acgggtcagc ttacgggtat cgatgttgta gatgcccaca atgttctgtt
    11341 ctttcaggaa tccgtcaata tccccttcca tgcggaagtt cgacggggtg ttgcaccatt
    11401 cccgcacaat gtaaccgtgg atataagaac gtgcagattc gctgtctgct gcattgacac
    11461 catagttgcc gatcagcgga aatgtctgtg tgaccagctg cccataatag ctggggtcgg
    11521 tcagtgtttc ctgataagca gtcataccgg tggtaaagac tacctcaccg attgccgttc
    11581 ccttgacacc aaaatgcaaa ccttcaaaaa tcgttccgtc tgccagaagc agataggcgt
    11641 tatcgcctcg ttccaataat gacatgacac cagtcctttc cgatctaaga gcctgcgatg
    11701 caccttgcaa tgcaccggcg ttgccctgaa aaattataaa tatgcggtaa tatcatcacg
    11761 catacggatt gcttctcttc tggcagcttc tgcaaaagcc gtttccggac agttttcttc
    11821 ctttttccat gcacacataa tggcacggga ggagttgaca atcgcacctc tgccgttttc
    11881 atcaaatgca ccggcaacgc cctgtgcacc gccgccctgt gcaccgtatc ccggcaccag
    11941 gaacagcgta tgcggcagct ttttccggag ttccgccagc tgttccggat aggtcgcacc
    12001 taccacagca cccactgcgg aatagccata ggcaccgatc tgcctgctgc cccagttttc
    12061 gcacatttcg cccatgatct catagaccgg cttgccgtcg atcagtctgt cctgcagttc
    12121 tccgctggac ggattcgagg tctttgccag cacgaaaata cccttgtcat atttttcgca
    12181 aacgtccagc agcggctgaa tgccgtctgt tcccagatag ccgttgactg tcagtgcatc
    12241 tgctccaaag ggttccagtt ccacgccgtc cacatctact tttcccagat gtgcggtggc
    12301 atatgcagtc atggtcgcac caatgtcatt gcgtttgccg tctgtcatga cgaacatgcc
    12361 cttgctctgt gcatagcgaa tggtttccgc cagtgtgcgg acacctgccc agccgtacat
    12421 ttcatagtat gcagcctgcg gcttgatcgc cggaaccaca tcgtacaggg catcgatcaa
    12481 cgccttgttg aactggagaa ttgcatcagc agctgcctcc agtgtatgtc ccagcttcac
    12541 aaagctggca tcttgtatga actgcggcac gtattccaga cgtggatcca gtccggctac
    12601 ggacggattg tgcagttcgt caattcgttt gataagtcta tcaaaagcca ttgcatcggt
    12661 tcctttcttc taaatactct gtatcctgat agcgaatgat gccgtctgtg atggtacaca
    12721 ccggtttgcc caccagtgtt ttgcccttcc agatcacatt tttcgctttg gagtgcattt
    12781 tttccggatc tactgtccag tgataatcca gatctaccag cgtcagatct gccggctcac
    12841 ctactgccag ctgatgctgc ggcaagccca gaatcttgcg gggattctct gccattttct
    12901 gtaccagctg cggcagggtc atcagaccgg catgcaccag tttcagcgaa gctgccagcg
    12961 aggtttccat gccgatcacg ccgttgggag cgtaacggaa atccgccttt tccgacggag
    13021 catgaggagc atggtcagta acgatacagt caatggtacc gtccagaagt ccctccagca
    13081 ctgccttgcg gtctttttcc gtccggagag gcggattcat acggtaatcc gcatcaccgc
    13141 agagcaccgc ttcctctgtc atcaggaaat agtgcggaca ggtttcacag gtcaccttga
    13201 caccctgtgc tttggcgtga cgaatgatct ccacgctgcc gtaagtgctg acgtgagcga
    13261 tatgaatcgg cacattggca gaagccgcca gaatgatctc ccgtgcggta atatagtctt
    13321 ccgaagcccg atccatgcct ctgacaccca gcgtttccga agccacgccg cggttcacaa
    13381 tgccgccgtt aataatggac agatcttcac aatgagaaat aaccggcagc cccagtgcat
    13441 cgccctgaat caaagcctgc cgcatggttt ctgcacattc taccggtctg ccgtcgtcgg
    13501 aaatggcaca gatgccggca tctttcaacg ccgcatagtc gcacatttcc tcgcctttca
    13561 gccccttggt aatgcagccc acggggtgaa tctccacgcc ggtataagct gctttttcgc
    13621 agatatagcg gacagtgtct acgctgtcaa ttgccggcga ggtattcggc atgcatgcca
    13681 caccggtgat gccgccagcc agtgccgctg cggaaccggt gaagatgtcc tctttatgtg
    13741 tctgcccggg gtcacgcaga tgcacatgca tatcaaacag ccccggcagt gctgtcaggc
    13801 gggagccgtc aatgacaaag tgggcagatt cgttcagatc ttttcccagc tttgcgatca
    13861 cgccgtcctg aatcagcatg tcacaacggg gcgaaatgcc gccctgcacc aattgtacat
    13921 cacgaatcaa aatggtttgc ttcattcttt cctcatttct gcatcagtgt tctgataaat
    13981 cacaacacag tcggttccgt caatttcccg cacggaaacc cgaacggttt cgctctgtgc
    14041 agtcggaacg ttctttccga tataatccgg acgaatgggt agttcccgat gtccgcgatc
    14101 caccagtact gccagctgca tgcactgcgg tctgccgatc gccagaatcg catcaatggc
    14161 agcacggcag ctacgaccgg tgtacagcac atcatctaca atgacaacac gcttgtcctg
    14221 taccggaaaa tgaatggtac tctttgcgga atgtgtggca ttggcaatgc ggtcatcgcg
    14281 gaatggggtg atgtccagtg caccgaattc cacctctgca cgttccaact cgtatatttt
    14341 acgggcaatc cgttctgcaa gcacaacgcc gcgggacaaa atgcccacaa tacatagatt
    14401 ttcggctcca cgattccgtt ccagaatctc atacgtaatc cgtgccactg cacggcgaat
    14461 ggcactttcg tccatgatga tcgctttttg ttccatacac acctcctaag agatcattca
    14521 caaaaaaagc acttacccgt ttcagacagg caagtgcgta caacaacttc tgcatcaaca
    14581 taccgtacat gcagaaggga tcttatccgg tttatgaaac gccttgtcaa tctcacggga
    14641 tcaacttaaa ggaatcgctc tttccgagtg catatccaac gcactcgttt ggtttttact
    14701 agtataccac attttccggc tcttgtccag tgcttttctg caatttccga aaaatttaca
    14761 ctttttccgc agttgctccg gacaaccgat tgctcagctc caccagctgc tccatggtca
    14821 ggttctccat acgtaccgtt tccggcagtt ccatggcatg caggacttcc agcagaaccg
    14881 gtttttcata gtgcatcgtt gcggacagtg cattcaccag cgttttccgc cgctgggaaa
    14941 aaccgtgttt cagcatggag aaaaagtgct ggggatcgcg tgtctggtcg gcatagcgtt
    15001 tctgcacatc gatgcgaatg acagcagaat ccacattcgg cggcggcagg aagctgtccc
    15061 gcggcacggt aaacagcatc tctactgttc cataatagtt cactgcggcg gtgatcgcac
    15121 cggagttgcg ggtgcccacc ggagcctgca atttttccgc cacttctttc tgcaccatta
    15181 ccgtaatgga atcgatgttc gctccgcttt ccagcaggtg catcagaatc ggcgtagtga
    15241 tgtaatatgg cagattggca cagactgcca cgtgcagacc ggcgaattcc tgttccagca
    15301 gctgcggcac gtcgaactgc aaaatatcgc cgtgcagaat gtgcaggttt tcgaattccc
    15361 cgacagtttc ttccagaatc ggcagcagcc gctggtccag ttccacggaa gtcacctttt
    15421 cggcacgcag tgccagctgc tgtgtcaggg tgccgatacc cggaccgatc tccagcacac
    15481 cataacctgc cgcagcgttg ccctcctcgg cgattcgcgg gcagacagag gggtcgatca
    15541 gaaagttctg ccccagcttc ttggaaaagt gaaacccgtg ccgtcccaga atcgaacgga
    15601 cggttttcat atctgttaaa ttgatcatca tgattttacc tcaatacttt tctgaagatg
    15661 tgtgctgtac agatagcact gccgcaccgg cattcccgtg atacactgta atgcaccgcg
    15721 gtacagcagc agctgttccc gatagctgtc cgccagttcc tccggcgaag aaacggtatc
    15781 ggtcttatag tccaccagca cgaagccgtc ctcctcctga aatgccaggt ctatgatacc
    15841 tttcaggata ctgtcggagt tttcatagcg gtgcaggatc gccgcagctt ccggatatgc
    15901 ctgcaagtcc cgacactgca ccagaaattt cttttcccgc agtaccatgt gggaacggcg
    15961 gagccgctga tacagctggt cacgaaagaa tgcttccgga atctccggct ttacgacagc
    16021 cgcctgctcc ggtgtcaaaa agccgtatgt ttgcagccgc cggatttccg caggaacatc
    16081 ggcttccgct ttccggaaat cggcgtactg gaaaaaggta tggatcgctg taccccgttc
    16141 cgcaccggta agcctgccct ctttctgccg gaaatccgga cgtttccaga caggagcacg
    16201 attttgtctt gcctcctgaa ttgccgaaac gctaagcagg gactcccgtt cgcagtccgg
    16261 cgatacatac tggaacgccg tgatccgacg caattcttcc agtgtttcct cgtccggcgg
    16321 agccgtcacc tctgcctgca attcttccgt ttcggtttcc tccggcagtt tgaattcgta
    16381 ggcaatttgc aggttttcgc tccacgccgg cgttttccag tactgcggcg ggaaattaca
    16441 tgcatctgcc aattccgcat cataccgcag gaacagacac atccagatcc actgtgccat
    16501 gctgctcact gactgtacca gatagacagg cagttccccc tccggcaaga tcattgcact
    16561 gcacttggtc agacagccgc ttttccccag ataggatttt ttccattcca gcggcaggaa
    16621 cagctgctgt tttgcacgtg tcattgccac atacagcaga cgcagttctt cacttttgta
    16681 ggcgttttca ccttcctggc acaaaactgc aaacggcaca gttttggcac ggacataggt
    16741 cgtcgggtct ttcacgcaga atccggcaag ccctctctcg gaaaacaggg cactcttttc
    16801 gccgtccctg tacttcatag ccttttccag attgccaagg aatacaaagg gatattccag
    16861 tcctttggag ccgtgcatgg tctttaccgc cacagcgttt tccgcaccgg cggaaacgga
    16921 cgactgctga aagtcatcgt tattttccag cagccagtcg atataccgca ggaagccgga
    16981 aacgctgccg tgaaatgcat cggtgttctc ctcatactgc ttggcgtatt gcagcagcag
    17041 acgcagattt gcccgctttc gttccccgtc ttttgccagc tgcattacag acaggaaatc
    17101 cgtggtgtca tagattcgcc gaatcagaga ttccagcgtc atcatgccgg agtcctgccg
    17161 cagcttttgt acggtgtcat acagatgccg gcacttctcc accaatactg aatccagctt
    17221 ttccgatgcg gactcttctg ccacaccaat agcgatctgc accgcgtgaa acagcttgga
    17281 ctttttggca agcatacgaa tctgcatcag ttcttccggc gtaaaccaga acatgggaga
    17341 gagcatcact gccgccagcg gcgtatccag tgtgggattg tccagcacac gaagcatgtc
    17401 gatcagaatg ctgatctccc gtgctttcag atagcccttt tcctccgggc tctgcaccgg
    17461 aatgccggct tcttccagag cgtgggcata tgccgcacag gtatcattat ttcgcagaag
    17521 cacgcagaaa tcccgatact gacaggggcg agttgtgccg tccttttgca ccaccggtgc
    17581 attttctgca atcatctgcc ggattttccg aacgacataa gtcatttctg catcgtattc
    17641 tttatccggt tccagaactg ccacgcagac tttctgctgc tctttcggca ggatctcgga
    17701 aatggcagag ccggcataca atgcctccgc ttcgttatag gtcacatcgc cgcaggatcc
    17761 cgtcatcagg ttgccgcaga cgaagttgac aaagttcaga atcgcaggaa cgcttcggaa
    17821 attctggttc agcacaatgg gattgcacac gctgtccggc tttccggcag aggcaatggc
    17881 atcactgaag ttctgcggat ttgccaggcg gaaccgatag atcgactgtt tgacatcgcc
    17941 taccagaaaa acgttttccc cgtaacgcag ggctccggtc tggggttcaa tgcaattgcg
    18001 ggagagcagc ttgaaaatat cgtcctgctt gttgttggaa tcctgatatt catcgatcat
    18061 gatcagctga tagtatgcct gcaactcctc tgccagcggg gacggaaaaa ttctgccgtc
    18121 tttttgcggc actgcgagaa gttccagtgc catgcgttcc gcatcgtcaa aggacaaggc
    18181 attctgccgc actttctctt tccagatttc cgcggaaatc tgttcttcca gttccagcag
    18241 cagcggcatc aactgtgccg tctgtgccag atcttccgcc gtataggggt aaatggtttc
    18301 tatctgcaac gccacatctt ctatcatgcc tttatacatt tcgcggagtg atttggcacg
    18361 tgcaagggct tcttcgttca gcaagccctt tttgctgctg gaaaatcttt tccctgtccg
    18421 ttcctgtttc agctttgcgt aaggttccag cagtttttct gcatctgatt cctgctgctg
    18481 caccatgcgg agcgtctgct ccagcatgtg cacgtctgct tcgatccacg ccaacgcctt
    18541 gttatccgta acagattcgt agagttccga agcagtatct gccgcttcat ttgccagccg
    18601 cagaatcatt tgcagatttt gttccatgcc tgccgacagc atctgataat aggcggaatc
    18661 ctccagcggc ttttgcagtt cctgctgcac ctgctgtacc cagttttcac ggaacggcac
    18721 tgtgcccaaa aacttgtgca gttccagcag cagcctttcc agatcggtgt cgtctttttc
    18781 acagaagctg ttccacaaca gctgcatatc ttctgcacgg tctgtgtgcc actggttcag
    18841 taccacgtcc gccgctttgg aagtcatcat tttgctttcg gtttcactca agatgcgaaa
    18901 gcctgcggtg atcccctgat cgcccagatt ttcccgaatc agttcaaagc agaaagaact
    18961 gatcgtgcag atatgggcgt tttgcaggcg gatctgctgc tggtacagcc acgtattttc
    19021 cggctgttcc tgcaatttct gatccagtgc ctgatacaga cgggaacgca tttctgctgc
    19081 cgcatcattg gtgaatgtta ccacaatcat atgatctgcg ggaactttgt tctccggtga
    19141 ttcgtccatc agaatccgca gcagacgttc caccagcacc gatgtttttc cgctgcccgc
    19201 tgccgcagaa acgatcagac cgctgtcccg tcgggtgatt gcttcttttt gctgttctgt
    19261 ccaatccatc agtgtttttc ctcctctgcc agttcttcca gaatcctcat catttcactt
    19321 ttctgaaggg tcgcacttcc cagccgcaga cgtttccggc aaaggctgct tgtaccgcag
    19381 atattggaga agtcgcagta ttcacatacc atcttgctgc ccatatccag cggcagagca
    19441 tcgatctccc cgcggtacac ccgttctgcc atgtatatca gctgatccag tacgtactgc
    19501 cgcagacctt tcatctgtcc tgtcgtcagg aatatgcctt tgttgagttc cagaccgcca
    19561 gtggcattca gctttccgtt caggtacacg ccgtcgccgt ttttctccat aagccgcagc
    19621 aggtgtgcat cttgcagcag aacgccgttc atctgatagg actggttgcg ttttgcagca
    19681 ggtgatacgg catcttcccg cttcaggttg cacttcacct ttccggcagg cagatacagc
    19741 acgcctgccg gctttgatcc tgccagtgca gtgccttctg acaaaatgga aaacagatag
    19801 atcagcatct gcatgtccag tccatagagc agatttccca gctcaaagat ctttccattg
    19861 gtcttatagt ctacgacacg cacccactgc tggtcgccgt cctgacagat gtccacacgg
    19921 tcaatcttac cgatcagctt tactgtctgt cccgcctcgg tgtgcagaca aattggcgga
    19981 aagttcggat tttcggaaga aatttgcagt tctgtgcagt acggcacaaa tgcagactgc
    20041 cggaattctt cctgcaaatg ctggcacacc ggcagaactt cttctacagc gtggtgatat
    20101 gccgcctgtt ctctttcgct cttggaaaaa tcaccgccca tgttctcctg ccagtacact
    20161 gccgcagatg tctctatctc ctgttgcagc tgttccgcag tgagtgccag aaagcttttc
    20221 ttgtcgtatt ttcggagcag ctgttccaga caatagtgca ccatgctgcc gtagttggca
    20281 gcattcatct gcaccttctg ccgctggtac agccggagaa tgtcattgca gtaataccgg
    20341 aacggacact tctgatagcg ttccagcgaa gatgcggaaa gctgcatggt gttgcccaga
    20401 tattgctgca tcagttccgg actggaaatc gaaaacagcg gggcatcttc tgaacgctgc
    20461 tcggcaagtt tccccagacg tttcagtttc tcggcaaatg acgcatcatc cagcagcagt
    20521 tgttctatgc tggctgtgtc ggtgttatgt gccgcataat tctgcacata ctgataatac
    20581 gccgcatgca tcgtcacgct gtaatacgaa ggttccagcg atgcacaatt ctgatgcagg
    20641 gtttccgcct ccggaaacgt ccgctgtatc tgtgcgatca ccgcagacgg gtaacacttc
    20701 tgatgagtca cgtctaccac aggataggtc agatacagtg catgggaggc agaggacagc
    20761 agcttatacg cggcaaggcg tgcatctgcc atctggcttt ccgccggctt tgccacagac
    20821 agccgcacgt cttccggcag gaattcgcag tcccgttccg aaaaaaccat actgccgccg
    20881 ggaaatgcag gaaatgttcc ctcacagaca cccagcagga aaacgatttt cggagcattc
    20941 agacgtgcgg tgctgccctg tgagatcagc acagcgtcca gtgtccgcgg cggcgtagca
    21001 cgctgaatgt tgccggtcaa cgccgcaaac actgtgctga actccgagaa ttccatttcc
    21061 agtgtccggt acagcgtgga caggtgttcc agaatatcga taaagctatt ccagaccagt
    21121 gcccactcct cggaagtctg catgcgtttc tgctcatctg caatcacttt cagctgacgg
    21181 tttaactgct cctccacctg ctgttctgtc aggaactggt acagcatgct gcaaaattcc
    21241 gcaccggtat gctgcccttt cagtttttcc cgcagttcct gcaatggggt caataatttc
    21301 agacgaaccg cttctgctga tgcaaaattc cccgcagtaa agggcgtatt ccacgttttt
    21361 ccgtcaattt gccacatata gcagtagttt tccagatcgg aaatttcttc gacggagcag
    21421 gaagtcagac cggtctttcc cagacgcatc agtaattctg tgtccggacg ggtttgccgc
    21481 aaaatgccca gcagcgtatg cagatacacc agcaggggcg tgtacagcat tgactctttt
    21541 tcgtccatgt agtaaggcag tttatagcgt tccattgccg tttccagaat gctccggtaa
    21601 tcagtcatct ggttggacag cacggcaata tcccgacagc ggagcgtgtg atctttcgcc
    21661 agcagccgcc gaatggttgc acagacgtag tccacttcct ctgtgggcga ctgtgcttcc
    21721 aaaatatgca ggtgcggtgc ttctccggaa aacggctggg tattccggaa aacgtgttga
    21781 ttcagccagc tgagttcagc tgcctgaaat cggtacgggg tatcgcacac ttccgtttcc
    21841 accggaatcc gcagcttttt tgccatgtcc tgaatcttgc aaaatgtttc ctgcacggcg
    21901 gcaaacagcg agaacggttc ctttcgggta tgctccatgc ggagtgcgat atataaatcc
    21961 ttgccggacg aaagcagcac agacagcatc tggtactcat cttctgtgaa gctctcgaat
    22021 tcgtccagaa agatcacatc acccagaaat gcgtcatgtc cattggcaat cgcagccgct
    22081 tcggacaagt ccgtttcggt atctttcagc tggtgctgtt ccagaagccg gtcataccgc
    22141 tgatagatct caaacacgtc cagcatcttt tcctgcaatc tgccgttcag ttccgcactg
    22201 gaattgtaca gctgttccgg cgggattccg ctgcggcgaa actgggtaaa catcattgcc
    22261 aattcgctga taaacgaagc ctgccggcac tgttttccca acagctgcaa ttttttctcc
    22321 cgttccgaaa cctgcaacac cgcctgatac agcagtgcca tttttgtcag ttcgtccgca
    22381 ttggtcttgc cgtccggcac gcagccgaac cgctggaata ccgcacgtgc cagagatttg
    22441 aagctgtggg tttccagctg gttaaatgca accggtccga ggatccggta gagtttctgg
    22501 tcaaattcgt aggaaaactg ctccggcacc agagtcagaa tctgttcgcc tgcttccagc
    22561 tgcttctgca aatgctgaat cagacatgtg gactttccgc agccggcacc accaataaga
    22621 atatgcagca aattccatgc acctcctgta attgaaaaag tggttcacgc tcgtgaaaaa
    22681 tagagtcact tctattatac cagctaaatc gcaaaaagtc aatttttccg caaagaaatg
    22741 caaaaaagcc gcaatgtgat acgcacactg cggctgtaca aaacgtattt ttacaaatgc
    22801 cctcacgaat tcggttccac gataatttcc cgatgcggca ccggcgtgga gaacacgatc
    22861 tgggtggagg tgtttccgaa acgctgaatc tttccgataa acgtatccag ttcctgcgtg
    22921 gacggaaatg ccaccttgat cagcatggag tacacaccgg taatgcagtt gcactccagc
    22981 acgttggggc agctgttaat gaacggataa aactccgact tctggtctgc cgacaactcc
    23041 agattgatga aagcggtaat atgataaccc agcttctgcc tgtcaattac ggtattatat
    23101 cccagaatga ttccctgctt ttccaactta tcgattctgg aagaaaccgc cggagccgac
    23161 aaaaatacct tcgaagcaag cagcttcaat ggtgctctgg cattttcctg taacaatgtg
    23221 attaacatgg catcaatttt atccatgaat ctatcccgtc cttcctttgg ctttcttttc
    23281 aagaaacctt atgaaacaaa ctttttccgg tgcatctctg cataaatttg cttcatgatt
    23341 ttcataattt aagtatagca aaaagatatg tctatttata agnnnnnnnn nnaagctata
    23401 ccatttataa gaaaaaatat aaattttcgg atttttcttt attttcttac ttttttacgc
    23461 tgttaatctt gtctttcgtt cgctgtactt cctgtaagtt gttccggaaa taatcctgtg
    23521 cttcatcaag caaacgattg acgtattttt ccgcagcatc tttaatgtca ttggagccgt
    23581 tctgtgcctt tgtcagcata tccagtgcac gctttttggc ttcctgcaaa atcgcctgtt
    23641 ccgagatcat gcggcttgca cgggtttccg cgtcctgtat gattgcttcc gccttttctc
    23701 tggcttcatt gataatgcgg tcgcagtcag aatcgatcag ctttgcacgc ttgatctctg
    23761 ccggaatatt cagatgtgca gaattcagca attcccgcat gcggtctgca tcgatcacag
    23821 ccttatgtgc ggcaaacgga atcgggcggg aacgatccag caggtcgtcc atatcatcta
    23881 aaatttcttc tatattcatg acagcttctc ctttaccaat cgttttacaa tcatatccag
    23941 tatttccggc ggcacaaagt ggctgatgtc accgccaaat gcagcgatct gtttcaccac
    24001 gctggaactc aggtacatat tctctgaagt tgtagtcaga aagacggttt cggctccggc
    24061 gtacagtttc cggttcgcca gtgccatctg gaactcgtat tcgaagtcac tcaccgcacg
    24121 caagcctttg acgatcgccg ttgctcctgc gtctttgacg taatccgcca gcagtccgtc
    24181 agaaatatct accgtcacat tgggcagatc ccgtgtaaca cacaagatca tgtccctgcg
    24241 ttcctgcggc gaaaagcagg cgttattctt catgatgttc ttggacacca acacgatcac
    24301 ctgatcgaac agcttcgatg cacgggtgat aatatccaga tgtcccactg taacgggatc
    24361 aaagcttccc ggacaaaccg caattcgttc catatgtatt tcagtccctt ctgcaaatgt
    24421 aggtggtgac tgtaatggca ccgtactttt tctgcttttt ctgttccaga acgcctacag
    24481 actggggcat ttccaaaccg gtttcgtgtt cgcagatgac aatgccatta tccgccatat
    24541 gttctgccaa cagcggcagc agcttttcat gcatgccctt tcgatacggc gggtccagaa
    24601 tggcaatgtc aaactgcatg gcagtggact gcaaaaacga ctgtgcctct gtctggtgca
    24661 gctgtgcctg ttctgcaaac tgtaccgatt ccagattttc ccgtgtcacc tgtagggaac
    24721 gtgcggaacg atctacaaat accnnnnnnn nnncaaatac cactgatgcc gcaccgcggc
    24781 tcaacgcctc gattcccatt tgcccgctgc ctgcaaacaa gtccagcact gccgcatcag
    24841 gcaaatcaaa ctgaattgcc gaaaaaatgg cttcctttac tttgtcggta gtcggacgca
    24901 cgtccatgcc ttccaatgtt ttcagtcttc ttcctcttgc actgccggca attacacgca
    24961 taccgatctc tcctttgcag atgtcatggg gaattcccgc atgacatgtg attttctcaa
    25021 agcatgttcc ccagggcaac acactctttt tattatacaa cgaaatgcgg caaatgtcca
    25081 gtattttttt gcaaagctta tgataccgct tttatttacc ctgtagaatt tccgccggtc
    25141 atctcctgaa tcacgccgta gagctcctca gcagttgtcc gattgatgcc ggcagtggtt
    25201 tccagttcct ccagtgttgc ctgtttcaga ttcaccagtg tcttgaaccg gagcaacagc
    25261 ttctgtgcct ttttctcccc gatgccacgg acttttgtca gttccatctg atatgaggac
    25321 ttgacgtgaa tgctgtgcat atacgctacg gagtaacggt gcacttcgtc ctgtatctgc
    25381 gtcagcaggt ggaatgcaga acgattggca gaaagggaga tctccccgcc ggcactggaa
    25441 atggcacggg tgcggtgctt ctggtctttc accatgccga acaccggaat gtgcagcccg
    25501 aagccgctga ttaccggtgc gatcgtgttc acatgtccct gtccgccgtc cagcagaatc
    25561 aagtccggca gtctggaaaa gccctcctcg tcctgtgcca gatagtgttt cagccgtctg
    25621 gtcagggctt ctttcataca ggcatagtcg tcctgtgtga catgctcttt catgcgaaaa
    25681 cgtttatagg cttttttcag cggtctgccg ttttcaaata ccaccatgcc gcagaccata
    25741 gaggtagagg agagattgga aatgtcgtag gcttcgatat actgcggcgg ctgaggtaat
    25801 cccagcacgg ctcccagttc ctccagtgcc tgcacttccc tgcccgtccg gttgaagcga
    25861 atcgccagtt cttcgttggc attattgcgg gaaagctgca caaactggca cagcattcca
    25921 cgttggggaa cggtgatatg cacccgtctg ccggcaaaat tggtgaacag ctgctcgtac
    25981 atctccggat cgggcatggc aaattccagc acaatatttt tggggatctc tccgcggtga
    26041 tgttcgtagt actgtgtcag gtaggagtcc atgacttctt cgcgggaggc gttttcctgg
    26101 aaaaagaacg tttccttatc aaaaagccgt ctgccgcgat accgcagaat ggacacacac
    26161 tgggtaccca tatgttccgc catggcgatc acgtcggctt cttccagagc cgcgtccatg
    26221 atcttctggg tttctccggc tttggtcact gcggcgatcc gatcccgcag cattgccgca
    26281 cgttcaaagt ccagtgcttc tgctgcacgc tccatttcct cctgcattcg ctttacggaa
    26341 tctgtgctgc ccatctggat atacgctttc gcctgctgaa tcacttctgc gtaatctttt
    26401 tgcgaaatgc agccgcggca aacgcccatg cactgcttga tgtgatagtt caggcagggg
    26461 cgtgtctttt tgaaatcctc cggaaaacgt ttgcggcacg tgggcagctg aaatacacgg
    26521 tttgcctctt tggcagcttc ccgtgccaca tagccactca tataaggtcc cagataggtg
    26581 ccggctttct tcttgttttt ctctaccgtc agtctgggat acggctcctc ggaaaagcag
    26641 agataggtat accccttgtc atctttcagc agaatgttat atttcggctg atgctgtttg
    26701 atcaggctgc attccagaac aagggcttca tattccgagt cggtgacaat aaaatcgtag
    26761 tcatacacat gctccaccat acttgccact ttgggggtat ggtcggctga ctcccgaaaa
    26821 taacttgtca cacgattttt cagattcttt gcttttccga tatagatgat ctcagacttc
    26881 tgatctttca tcaaataaac acccggcgac gttgttaact ttgcagtttt ttcccgcagt
    26941 acaggtaaac gacaattcat acaacgcctc cgagtgttga ttttccatta caattgataa
    27001 tgtattaaga cagtgccttc agtgcacgct gtccgatgtc cttccggtaa tgtgcatctt
    27061 ggaacgaaat ggttttaacg cctacataag cagtgtccag tgcctccggc agagttgccg
    27121 cagttgctgt aacgcccagc acacgaccgc cggctgtcag gaatgtgtcc ccgtccagct
    27181 tggtgcctgc atggtaaaca aagcagtttg cagtctgtcc gttttcgtcc agaccagcga
    27241 tcggaagacc ggtagcatac tttttcggat agccgccgct tgccataact acgcaggctg
    27301 cacagccgtc tttccactga atgtccagct gatccaatgt accgttccag atcgccagca
    27361 taatgtctac cagatcggta tccagcagcg gcaaaactac ctgtgtttcc ggatcgccga
    27421 accggcagtt atactcgatg accttcggtc ccttgggtgt cagcatcagt ccgaaataca
    27481 gacagccggt gaacggtctg ccctcctcct gcatcgcacg aatggtggga aggaaaatgg
    27541 tatccataca ctgctgtgca atttccggtg tgtagtacgg gttcggggca acagtgccca
    27601 tgccgccggt gttcagaccc tgatcgccgt ccagtgcacg cttgtgatcc atggaagaaa
    27661 ccatcggctt aacagttttg ccgtctgtga agctaagcac agaaacttcc ggaccagtca
    27721 ggaattcttc gatgacaacc ttggaaccgc tttccccgaa gatcttgtct tccatgatcg
    27781 atgcgatcgc atcaaaagct tcctgttcat tctgggcaat gacaacgcct tttcccagtg
    27841 caagaccgtc tgctttgata actgtgggat attcgttctt ctctttcagg taagcttcag
    27901 cttcctgcgg cttgtcaaat acacgatatt ccgcagtcgg aatgtgatac ttctgcatca
    27961 gttccttgga gaacaccttg ctgccctcca gaatcgctgc gtttttcttc ggtccaaagg
    28021 tcatgaagcc ctctgcctgc atcgcatctg ccatgcccag taccagcgga tcgtccggtg
    28081 caacaaacac cagatccact gccagttcct ttgccagctt gaccataccg tcaatatcgg
    28141 ttgcctttac cggaaaacac ttcgcatact tggcaatgcc gccgttgccc ggtgcacagt
    28201 aaagtgcctc gacctgcggg ctctctgcca gtttcatgac gatggcgtgt tcccgtccgc
    28261 cgccgccaac cactaaaatc ttcatgctgt cttcctcctg cgatcagtga tggaacagac
    28321 gaatgcctgt gaacgccagt gccatattgt acttattgca tgttgcaata acatggtcat
    28381 cacgaatcga accgcccggc tctgcgatgt actgcacgcc actcttatga gcacgttcga
    28441 tgttgtcgcc gaaggggaag aatgcgtcag aaccgaggca aacctctgtg ttctgtgcca
    28501 gccatgcctg ctgttcttcc ttggtgaaca cttccggctt taccttgaat gtattctgcc
    28561 atgcaccttc tgccagcacg tccatatatt catcagaaat gtacacgtca attgcattgt
    28621 cacgatccgg acggcggata ccgtctacga actgcaagcc cataaccttg ggatgctgac
    28681 gcagccacca gatgtctgcc ttattgccgg caagacgtgt gcagtggata cgggactgct
    28741 gtccggcacc gataccgatt gcctgaccgt ccttgacata gcatacagag ttagactggg
    28801 tatacttcag cgtaatcagg gaaatcagca gatctctctg atatttttcc ggaatttcct
    28861 tgttgtcggt aacaacattg gacagcattt ccttgtcgat cttcaggaag ttatgtccct
    28921 gttcaaaggt aacgccaaat acctgcttcc gttccagttc tgccggacgg tatgccggat
    28981 cgatctgaat gatattatag gtacccttcc gcttggactt gaggatttcc agtgcctccg
    29041 gctcatatcc cggtgcaatg ataccgtcgg aaacttcccg ctggatcatc tttgcagtgg
    29101 gcacgtcgca aacgtcagac agagcaataa agtcaccgta agaagacata cggtctgcac
    29161 cgcgggcacg ggcataagca cacgccagcg gagttcgccc agatcatcta cgaaatagat
    29221 cttcttcagg gtatcagaca gcggtgcacc aactgcggca cctgccggag aaacgtgctt
    29281 gaaagaagtt gccgcacaca tgccggttgc ttctttcagt tccgatacca gctgccagcc
    29341 attgaaagcg tccagcagat tgatatagcc cggcttgccg ttcagtacag tgatcggcag
    29401 atcgctgccg tcctccatat agatgcggga cggcttctga ttcggattac agccgtattt
    29461 taattgcatt tcactagcca tgaaagtgcc tccttactga ttcttgttta cgattctggt
    29521 ttcagttttc ttggttttga ggtcgatgta ccgaacgaac agagaaacct tgttctccgc
    29581 attcaggctg ttccacagca tttctgtgaa cgcatcgatg tcaccggaaa ccgcaatttt
    29641 ctgcggttcg ccggaaaagc tcggcagcgg atttccatca ccctcatagg tgtgaatgaa
    29701 atgtccaacg ccggcaatgg gattagaata ggagaatgta tatctctggc aggaggaagg
    29761 atcgccgtct gcacttttca gaatagacag tgcataggaa aagccattat tgctctggtc
    29821 gatcagtccg gagatacgcg gcgtatagtt cggtgcgtcc ggttcgaact cacgggtccg
    29881 cagggattcc tcaaaagttt tcccagcctg catcaaatca tacacagtgt ctgtctggtc
    29941 gccattggtc acaatggtat aatctcccag gacgcgaacc ggtgcgtaga tgatcaggct
    30001 cgggtctacc attttcgacg gatcaaatgc ctgtgtgcgg atacctgcac cgtcctctac
    30061 gaaaacgcgg ttccggctgt tttcacttct tcccatgata aagtatgcaa aaaccgcata
    30121 cgctccgtcc tccgaacgtc ccagcacaat gccgcggccc ggataagaat tctgctgcaa
    30181 ttcctgctgc aatgatgcaa tcttcataat ttcctcctac tgatattgcc tttactgctt
    30241 aatgaatacg gttttctctc gaacttctaa ctgtccgttg cagaacaggt tcactgcctg
    30301 cggcagaatc tgccactctg cctgttccat gacacgcttt tgcagcactt ccggtgtgtc
    30361 gtccggaaat acagcaacag gctgctgtaa aatgatcggt ccgtcatcgg cgacttcgtt
    30421 gacgaagtgc actgtcgcac cggtaaactt cacgccccgt gccaaagctg cctcgtgtac
    30481 atgcagtccg tagaatccct ctccgcaaaa ggacgggatc aaagacgggt gcacgttgat
    30541 gatctgatta tgatatgccc gaatgatcga ctgatccaga atggtcatga agcctgccag
    30601 tacgatcaga tcggcatatt cctgaaacag tgcttccagc agtgccttgc tgtacatttc
    30661 acgagatgca aattctttcg ggacgataac gcgggtgcca atgccgtttt ctttggctct
    30721 ggtcagtgcg tatgcgtccg gattggagga aatcacacag gtgatttttc cgttcggaat
    30781 cttcccgttt ttctgtgcat cgatcaatgc ctgcaaattc gttccgccgc cggaaaccag
    30841 cacaacaata tttttcattt tgctctgcct ctttctatta cttgaggatc acgccatcat
    30901 cagatgcaac cagttcgccc agcacatagg catgttcccc tgctgcattc agaatacgga
    30961 ttgccttatc tgcatcttcc tgagccacgc ataccatcat gccgacaccc atattaaagg
    31021 tgttgaacat gtcgtgttcc ggaatcttac cagtctttgc gatcaggtcg aagatcggca
    31081 gaacctgcac gtcgctgcgg tcaatctttg cagacagacc ctttttcagt gcccgcggaa
    31141 tgttctcata gaaaccgccg ccggtaatgt gagcgataga cttcggctgt acctctgcca
    31201 gcagttgctg aatcgcgttg acatagatct tggtcggtgt cagcaggcag tcgcccagtg
    31261 tggtgcccag atcagagaag tgacgagcca gattctgttc gttgactgtg aatacgttcc
    31321 ggatcagaga gaatccgttg gaatgtacgc cgctggactg cattgcgatc agcacatcgc
    31381 cttccttctg gctgtccatg ttcaggatct tgctcttatc tacgacacca acggcaaaac
    31441 cggcaacatc gtattcatct tccggataga agcccggcat ctcggcagtt tcgccgccga
    31501 ccagtgcaca gccggactgt acgcagccct ctgccacacc ggaaacgatc gatgcgacct
    31561 tttccggaac gttcttgccc agtgcaatat agtccaggaa gaactggggc tttgcaccgc
    31621 agcagataat atcattgacg cacattgcca cacagtcgat accaatggta gtatgcttat
    31681 ccatgagaaa ggcgattttc agctttgttc ctacgccgtc tgtaccggaa accagtaccg
    31741 gctgctgcat tcctgccaca tccagtgcat acagaccgcc gaagccgcca atgtctgtca
    31801 gcacatttgc tgtcatggtt ctggcaatat gggacttcat caactccact gccttgtaac
    31861 cggcagtgac atctacgcct gcctttttat agctttccga atagctttcc atttatgtgt
    31921 tacctccaaa gtttgtcggg cgaatccgat acggaatcgc cgcaggaaac cgcctttcgc
    31981 aatttcccga tcattcgcct atcttctttt caaatttatc tttcggcatt tccttcggca
    32041 cttcgatagg ataattgccg gtgaaacatg ctgtacaaaa accagtctgc ggtgtcatgt
    32101 ccggcgttgc cagcgactgc acaccttcta tgctcagata ccccagtgta tctgctccga
    32161 tatgttcaca gatctccgga atggtcatct ggcatgcgat caggttttcc ttactgtcaa
    32221 tgtccacgcc gaaatggcac ggatttgtaa acggcggaca ggagatccgc atatgtactt
    32281 ctgttgcacc agcttcccgc agcagactga cgatccgctg gcaggtcgtg ccgcggacaa
    32341 tactgtcatc taccagaatg acacgcttgc ctgccacaac gctttttacc acattcagct
    32401 tgatccgcac ggaatccgtc cgcatcttct gattgggctg gataaaggtt ctgccgacat
    32461 agcggttctt gataaagccg acttcatatg ggattccaga agcctgtgac agtcccagtg
    32521 cggcatccag tccgctgtcc ggtacaccga taactacatc cgcttctacc ggatgctctt
    32581 tccagagcag ctttccggca cgaatgcggg catcgtgtac ggtacagcca ttgatgacag
    32641 aatccggacg tgcaaagtac acatactcaa acacgcacaa agagggcttc tgtccgcagt
    32701 gtgtccggac agagcgaacg ccgtcgctgt ctgcaataac aatttcgccg ggatccagtt
    32761 cccgttcgaa ctcagcctct acgctgtcca gtgcacaggt ttccgaagca aatacgatac
    32821 tgccgtcttt ccgccgtccc atacagagag gacggaagcc gttgggatct ctggcagcga
    32881 tcagcttctg cggggacata acgaccaggg aataagcacc tttcaggtca tacatggcgt
    32941 attccacagc ttcttcaata gaagaacgct ccagtctttg ttccgtcacc gcataagaga
    33001 tcacttcagt atcgttggtg gtgtgaaaaa tcgcaccacg cagttcatac tttctccgca
    33061 gctcatgtgc attgaccaga ttaccgttat gggcaatcga catgggacct tttacatggc
    33121 ggaccacaat gggctgtgca ttggaacggc tcggctttcc ggttgtggaa taccgcacat
    33181 gtccgatcga aatgttccct tgtccgagtt tttcgatctg actgcggtta aatacctccg
    33241 gcaccaatcc cagatcctta tgatgcgaga aaacgccacg atcgtttacc acgatcccgc
    33301 agctttcctg ccctctgtgc tgaagtgcat acagtgcaac atatgtcatc gccgcaacgt
    33361 ctgtggtgtg cggtgaatag acaccaaaca caccgcattc ctcatgcagt cctgtcttca
    33421 atacaatcat tcccttccgt tccagcaata cgaacagagc tgacagtcgg gttttcctac
    33481 ggcacgaatc gtgccttcca gcgattgaaa ttccaatgac gtcagttgca gacgccgaca
    33541 gatttcgtcc cgaagcagct ttccacgctc tgtgttagag ttgctgtact cgtcaatatg
    33601 ctgcaaaccg gcaacgccct cattttcatc aatgatctgg cgtgcgatca gttccatctc
    33661 cgaagtgctt ctggagaagt tcagatattt acagccgtac ataatcggcg gacaggcaga
    33721 ccgcatgtgg acttccttcg caccgttttc atagagaaac tcaaccgttt cccgcatctg
    33781 tgtgccccta acaatgctgt catctacgaa aagaagtttc ttgccccgaa tgagttccgg
    33841 aacaggaatc agcttcattt ttgcaacccg attccgcatg gactgattgg ctggcatgaa
    33901 gcttctcggc catgtgggtg tatacttgat aaacggtctt gcaaatggaa tgccgcttcg
    33961 gttggcgtaa ccaatggcgt ggggaacacc ggaatccgga atgccgcaga cataatccac
    34021 gtccggaagc tttccgtttt tcatatcgtt ctccgccatg atctcaccgt ttttggtacg
    34081 catcacttct acattcacgc cctcgtagca ggcggtggga tacccgtaat acgtccagag
    34141 gaaggtacag atcttcatct cctccgttcc ctcatagaga acttcgtagc cgtccttggt
    34201 cagacaggtg atctcccctg ccttcagttc gtggcaggtt tcatacccca gtttctggta
    34261 tgcaaaggat tcgaacgaga agcagtagcc gtctgaacgc tttccgatca gaatcggcag
    34321 ctttcctgcc cgatctcttg cacagataat ggagtcctct gtcatgatga ccaaagacat
    34381 ggtaccttca attttacttt gagcatagcg gatcccctcc acgaaggtcg gcttctgtgc
    34441 gatcaacgca ccgatcagac cgccggagtt gatgttaccg ctgctcatgg tttcaaagtt
    34501 cgcacagccg ctttccagaa gttcttcgga aagtgccttt gcattctgaa taatgccgat
    34561 cgagcaaact gcataaagcc ccagtttgga acgtaccatg atcggctggg gatctgtatc
    34621 actgatacag ccgatgcaga tattgccccg cattttttcc acgtcctttt cgaactttgt
    34681 ccggaaaggc gagttttcaa tgctgtgaat attccgctgg aatcccagtt ccgcagaata
    34741 ggatgtcata ccgccgcgtc gtgtccccag atgtgaatga tagtccgtcc cataaaatac
    34801 gtcgagaacg cagtcgtttg ctgaaactgc accaaaaaag ccacccatgg attattctcc
    34861 aaagatccgc ttcataactt cctgataagc ttcttctgta ccgcccatat ctctgcggaa
    34921 acggtcctta tccagcttct cgttggtctt tacgtcccac agacggcagg tatccggaga
    34981 gatctcatct gccagaatga ttgtaccgtc agacagacgg ccgaattcga tcttgaagtc
    35041 gatcagacga atgccgatag acaggaagaa cttcttcaga acttcgttga ccttgcgggt
    35101 gtaattcttg atcgtgtcga tttcttcctg tgttgccaga cccaatgcca gtgcatagtc
    35161 atcattgata aacgggtcgt tcagttcgtc gcacttgtag ctgaattcca gaatcgggca
    35221 cagcagatct ttgccttctt ctacgcccat acgcttggag aagctgccgg ctgcaacgtt
    35281 gcggacaata acttccagcg gtacgataga aaccttcttt acagcagttt cgcggtcgct
    35341 cagttcctct accagatgag tcggaacgcc ttccttttcc agctgcttca tgatgtagtt
    35401 ggacatacgg ttgttgataa cacccttgcc aacgatcgtg cccttctttt cgccattgaa
    35461 tgcagttgca tcgtccttat agtctacgat cacaacttcc ggattctcgg ttgcgaatac
    35521 tttctttgcc ttgccctcat agagctgctc tgtttttttc caatcagcca ttttctgttc
    35581 ctccaaaaaa attaaattta tatcattatg aaatcagcag ttactgtatt ctgctgaaat
    35641 ttttgcgtct tttgccttta cgccctgttc catgtctgct ttcatctgtt ccagctttgc
    35701 gtcgatttcc ggatcagaca gtgccagcat ctgtgctgcc agaattgcgg cattggctgc
    35761 cccgtcgatt gcgacagttg ccaccggaat gcccttcggc atctgtacgg ttgccagcaa
    35821 tgcgtccagc ccgtccagtg tagaagactt gaccggaatg ccgatcaccg gcagcgtggt
    35881 atgtgccgcc agaacgccgc ccagatgtgc agcctttcct gcggcggaca aaatcacgcc
    35941 gaagccgttt ttcttcgcgt ttccggcaaa ttccgcagcc agtgccggtg tccggtgtgc
    36001 ggacatgaca tgcacttcat acgggatccc gaacttgtcc agttcgtcca gtgcaccctt
    36061 tactaccggc agatcgctgt cactgcccat gatcactgct actttttttg acatatgtat
    36121 tcctcctgaa tcagagttac acaccgcttg ttgcaagccg gtgtgctgtt gcggcaaggc
    36181 agtatgcctc gccggtttat gaaagcagaa tgcgaatcaa gaaagcttcg cctctgttat
    36241 cacccatgtt tcttttccgt tcttttccat tttgaacgtc agtgtcttat tttccgacgc
    36301 attttccgca ttgcggagcg tcagcccgat ctgccatgtc agaatcagtt ttcccgcctc
    36361 gttttgttct gcctgcaaat acacgtcaga atccatacgg gtcaccctgg catccgccgg
    36421 agcgatattc cccagaacag cattttcatg atcctgtccg gtaaggtcct gcagtttgga
    36481 aaaatcctgc attttcacca tatccagaaa cgccgctgtc tgatcggcac tgctgctcca
    36541 ttcttccggc agcagcagcc cggcatgtac tttttcagca tggacagaaa acgaaatctt
    36601 ttccgttttc cgtgcaaggt cacactgcac cagctggagg atatcttttt ccagagagaa
    36661 ctgaaacgaa tcttccccgc tggtgactgt cagcaatggt ttttcccatg tgctgctgtc
    36721 ccagaccata gcatctgctg cacggaaatc gtggtcatac aacaccacct gctggttttc
    36781 cgccagcacc gccagataat aggaatctgt caacgcctcc acatggtcat agacgaaagg
    36841 agcgaccacg ttgccgtcca gatccagcac accaacacaa ttgctgtcag cattttcctg
    36901 tacggcagaa aggtaatccg aaccgataaa atgcaattct ttccattccg gttccagaat
    36961 caggttgcct gccgcatcgg atacaccgta gagatgatcg tccccctcta caatgacatt
    37021 gccgttggaa ttcgttcccg tgtcaaaagt actgttctga gagccggcag actttttgga
    37081 gatgtttatc gaaaagctga cagttgccac gatcagaagc accagaacca atacgagaat
    37141 gcctaccgcc agacgggtat gtttgttcat ttactgttct cctgccgcgg tatccatatc
    37201 cttgcgggca tctttttcag tttcctgtac cagatagatc gggcgttttt tggtttccag
    37261 ataggttttt gccagatact gaccagcaat gcccacacag aacagctgca agccgcccat
    37321 gaagaaaatt gcacagatca aagtggcaaa gccctgtacc ggatcaccga atgccagctt
    37381 tttgatgatg atgacaataa tcatgataaa tgcaatcaga cagcttgcca cgccgaggat
    37441 cgaagaaatt gtcagcggtg ccgtggaaaa cgcaaagaag ccgtccagtg catagcggaa
    37501 cagtttccag aaagaccatg tgctggtacc tgctgcacgt tccacattct cgtattcaat
    37561 atatttggtg cggaatccca cccagctgaa aatgcctttg gaaaaccggt tatactcttt
    37621 catagccaga atggaatcta ccatctgccg tgtcataaag cgaaaatcct gtgcaccgtc
    37681 cacaatttcc gtctgcgaaa tccgcttcat gatcttgtag aacttgcggg agaaccacga
    37741 aagcaatttc ggctcccctt ttcggctgac acggcgggtc gtggcacagt catattcgcc
    37801 gcttaaaaca atgttataca ttttcggcag gaatgccggc gggtgctgca agtccgcgtc
    37861 catgatgacc acataatctc cggtggcatt ttccaagccg gcatacatac cggattcttt
    37921 tccgaaatta cgggaaaacg aaatgtagcg aacgcgttcg tcctgcttcg ccagttcccg
    37981 cagctttgcc agtgttccgt cacgggagcc gtcattgata aacaacagtt caaattcggt
    38041 atctgcatgt tcttcctgaa tctgctgcat cactttctga atttctatgt aaaaaagtgg
    38101 caatacttcc tgttcattat aacatggtac cacaattgaa atttttttca tagcgatcct
    38161 ttccgttcca cagccgtcca tataaatatc ggctgttata ttcggcgttc ccccaggaca
    38221 acaccgcaca ggctctggca gtattttcct aaaatctttt ctcctttggc acgcctttat
    38281 ttattatacc accttttctg acattttaca ataccctaat ccggatattt ttttcgattt
    38341 tcaaaaaaat atgagaacaa atcaggattt tccattttca cggtgtttca gaagatcaat
    38401 ttcagctccg gtgggacagg aattctgtca gccgccgctt ataattcggg gaacggtggg
    38461 acatttgttc cagcaagttc agcagttcct tctttgtcac cagccgcaga tcctgcactt
    38521 ctgattcctg catggtgcag tcttgcaggg aaaaaggaat ctgtatatag tacacatcat
    38581 ggtaacagct gcggcgtttg tcccgaaaca ggaactgcat cttctctctg ggaagtgtca
    38641 gtccgacctc ctctttcgcc tcccgcaaag caccgtccag actgacttca ccgcagtcca
    38701 ctgcaccggc agtaatatcc cactgtccgg gacgtgttgc ctttgtgtct gaacgctgct
    38761 gaatcaggaa cagaccgtcc gtgttttcca gaatcacatg ggcacacagg aaatattccc
    38821 ctttagacaa aaaggtgccg cgttctgctg tttttccggt tcggttgccg tgctcatcat
    38881 aaatatccag ccattccatg ctttaccaca ttcctttcat catgctgctt tacattttga
    38941 ttgcaattct attgtaacat gaaatctttt ccggcttcaa gaagcatttc tgaatattct
    39001 ttgaaaaaag ccgctttact gcgataaaac gttcccgctg tctttccggc atcagcttcc
    39061 gtacaagcgt tgcacagcct tttctgaaaa ttgcatgaaa atttggaaaa tccgcttttc
    39121 attcctgcaa gagtgtggta taataatatg aaatacactt ttgacggccc tgtttcctta
    39181 caaaaggagg ttttccctat gtccaatttc agagaacggc tgatgcaatt catgcgtggc
    39241 agatacggcg gg
//
LOCUS       DAWBGR010000003        36931 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_471_length_36931_cov_7.812724, whole genome shotgun sequence.
ACCESSION   DAWBGR010000003 DAWBGR010000000
VERSION     DAWBGR010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 36931)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 36931)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..36931
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_471_length_36931_cov_7.812724"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    13499..13508
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    28108..28117
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9484 a         9163 c        10074 g         8190 t
ORIGIN      
        1 caataccgca caaagagcgt ctggcgactt tgcacgccat ctgcttgcaa atttctcgtc
       61 atatttcaca aaaatgctcg tgaatacatc aagtattcac tccgcttttt gtttcatctg
      121 acgaaaaatt tgacgacgca tctgtcgcac aatctttgtg cggtattgcc aaaagacaat
      181 cccccatttt caagaaagga caagcatatg aaacagacta cgaaaaaact tgccggactg
      241 gcttgcggac tggctctggt gctgggctgc ggtgcaggaa cgctgacggc ttcggcgacc
      301 tctgtgggtg atgtcattgc tcacgcctat gctgtagggc tgccggagtc ggacattcag
      361 gcatgtatcg cccagtacag cggcagagag tacacctccg aacagtgcga tcaggcgatc
      421 gccgcattgg acaactgggc aaaagagcga gatgacaaga tcaacgagga gatcaataag
      481 ggcaccacaa cggctgctcc ggaagaaaac cgcaccacgg aaaccacggc agcaactgcc
      541 gcagcggctg ccggcaaaac tgcacagacc actgctcctg ccaagaaaga tttcctgaac
      601 atgacgctga acgaaaaggt ggagtacatc aacagcctgc ctgccgacca gcggaacgac
      661 tttgtacaga acatgagcaa cgaggagcga aacagcttcc tcaagcagat gaagaccagc
      721 gaccagctgg acgtgatctc cgccatgaca gacgtgggaa aggcgttcgg cgtgaacttc
      781 tctgtggaca gcgtttccga tgatgccatt tctgtcagca ttcacgacga aaacggcaat
      841 ctggtaggcg tttccaccta cggcaacacc gtggaagcta ccggaattcc ctacaccgtg
      901 ccggtactga tcggcggcgg tgcgatcctg ctggcatctg ccggactggg agccttactg
      961 ctccgcagcc gccgcactgc cggacaggca ggaggaacgc tatgaaaaag aaaacggatt
     1021 ccaccatttc tctgcctgta tgggcacagc ttctgctgcc ggttctggtg acagtgctct
     1081 gtgccggcat tgtgctgctg tgcagtatcc gcccctatga acaggcaaaa acctacctca
     1141 aagtggcttt catggcaggc agtacctctg ccgccggaga gggcaccaac gggctgaaca
     1201 ttgtccagac ggacattgac accgactaca ccggcaagac ggattccgag ggtacgcctg
     1261 ccgcagtggc ttacggcagc cagtgtgctg tgctggaagc gaaatccatc gggctgtacg
     1321 ttcccgtcta ctacggcggc ggctcggaac tgctggagca gggagcgtgt ctgaccaccg
     1381 cttcggctct gctgggagcg tcgggaaact ccgtcatcag cggacacgtg aacacctttt
     1441 tccacgatct gtcccagctg caagtgggcg acacagtgac cgcctacacc acctacggca
     1501 aattcaccta tgaagtcacg gagcagatcc agtttgaatc caccagcaag tcctatctga
     1561 agcagacaga cgacgaccgt ctgacactgt acacctgtga gatgcagctt ctcggctcgt
     1621 cctcgaaacg ggtgggcgtg atctgcaagc tgacggataa agcattctac accgacaccg
     1681 aagccggaaa aggaggtacc caatgaaaac tgaaaccact tccggcggca tgggcaagct
     1741 gatactgcgg ctgatcctgt cgatcctgct ggtattttcc ctgctgggaa cagtcggctg
     1801 tgccgtgggc atttccgtcc tgcacagtcc gtcccagctg attgcccaga tgcacaaaca
     1861 aaacgcaggg caaaaggtct acgacagttt acagacccgc ttcaccaccg actacaacac
     1921 cactgccgtt cctgcgaatg tttacatgga cgcgatctct gtggactggc tggagcagtg
     1981 catggagcag aagctgacgg ctctctacgg agcggacagc gatctccttg atttttcggc
     2041 actggagagc agcatcacgg attactttga gcaatacgca gaagaaaacc actacgtcaa
     2101 ggacgatacc tacaacgaga agttgcagga aaccattgac aacggggaaa agatcatctc
     2161 tgatgccaca gacctgctgc ggaaggacac gctccagaag gccggctatc tgtccaagct
     2221 ggaaaagctg cggacgctga cttttgcggg tgcaggcgtt tgcggcgtgc tgacggtact
     2281 gctgctggtg ctgctgcgga acagctactg gatcggcacg ggctgcttcg gagcaggggc
     2341 actgctgact gtcggagccg gcgtggtttt cggcacaggc gtgattcacc ggttcgtgct
     2401 gaaagaggcg gctgtttacg cagtagtcac cggtacgatg actgccctca ccacggcagt
     2461 gctggtggct ggcatcgtgc tgttggcaat aggcgttgta ctgttagtgc ggcgggtcag
     2521 cctgtcggga aagttttgtt gacgaaaatt cacaaggcaa aaaagcctcc cctgaaaggg
     2581 gaggggaacc gccgttaggc ggtggagggg tttccacaca atagcctccc ttgaaaaggc
     2641 acccgaaggg cagacgaagg gatttcttct gtgaaacctg aaacagaagt aaaagattcc
     2701 acagaacccc tccaccagct tcgctggtcc ccctcccctt tcaggggagg ctcatttgta
     2761 tctaggctcc cttgaaaagg cacccgaagg gcgtgccttg tgctgagctg acagccgcag
     2821 gctgactgag ggattcttct gtgaaacctg aaacagaagt aaaagatttc acagaacccc
     2881 tccaccaccc tcattccaca acaacaaaag ccgtacccga aaaggtacgg cttttttagg
     2941 caatcccgca caatctttgt gcgggattac ctttaggaaa cactgaataa agcccgcctg
     3001 acggctttgt acgcaatctg cttgcagatt ttccgtcata ttgcccagaa atcccttgcc
     3061 agacatacag tctgcctgcg gaatttctat gcaatctgcc aaaaaatctg actacgcatc
     3121 ttacgcacaa gctttattca gtgtttcctt tattctattt caccagattg atgaccggta
     3181 tgcctttttg ttttgccata cgcacagtct gagccgtgcc gcttctgccg gtggggtcgc
     3241 tgcaatagca gatacagcag tccgccagtt ccaccagccg ctggttccgc agccgcatgc
     3301 agcttttcgt gtattcctcc gacacgaatt cgcaggaatc cgcttcccga aaaatgcagt
     3361 cataggctgc cttttgtgcc tctgtccagc gtgccgtctg ctccccgcgg gaacagggca
     3421 gcaccagatg cagggcaata cagggatact cctcccgcag atccagcacc gtctgtgcac
     3481 aataggtatc ccaccccagt gcaccgccgg cgtagaagtc cacaaagccc tgcggaatgg
     3541 cagtttccag cacccgccgc agctctttca gcagtgccgg cagctctgtc ctggcaatat
     3601 gccggtgacc ggtaaaacaa atggaacgca ccattacacc tgattctcct gctccatttt
     3661 ggcgttcgcc agcatcagct tgatgcggtt ctcctgattg acgcgggtcg caccggggtc
     3721 gtagtcgatc gccacaatgt tggcattggg ataggcgttc ttaatggttc tcgccatgcc
     3781 ctttgccacg atatggttgg gcagacagcc aaagggctgg gtgcagatga tattctccgt
     3841 gccggtttcc gccagcactg ccatttctgc cgtaatcatc cagccctcgc ccatggaaac
     3901 gccctgactg atatacttat ccgcagcacg gcggaggtct tcaaagtcgt ggggcggggt
     3961 gaattcgccc tgcttctgga tcgctttgat catctgcacc tgcttgctgt ggaggaactt
     4021 ataccccagc ccgaacgcca gcgtcttgaa gttcttgtag tcgtacaggt ggctgttggt
     4081 ttcgctgttg gcggcacagt agaggataaa ttccagcagg gtgggaacca ccggctcaca
     4141 gccctcggaa atgaggaagt cctccagatg attgtttgcc agcggcgaat acttgacgta
     4201 gatctcgccg ataatgccca ccctgatctt gggctttttg ctgcggggga tcgccgcgaa
     4261 gtccttgagg atctgggcgt agatcttttc cgcagagagg aacttgttgc tctcgaacag
     4321 cttgggcagc ttctcctgcc atgcgtccag cacctgctgg gactcgccct cgttcagttc
     4381 ataggggcgg cactggttgt acagcgtcat cagcagatcc gcatagagga cagcgtagac
     4441 cattttgagg cacagtgccg gtgtcagttc cagactacag tccttttcca gtccggcaaa
     4501 gttcagggag agcaccggca cctgcgggaa ctgttccgcc atgcacttcc gcagcaggtg
     4561 gatatagttg gacgcacggc agccgccgcc ggtctgggtg atcagcaggg cggtgtgatc
     4621 gacatcatac ttgccgcttt tcagggcttc catgaactgt cccacgacca gcagggcagg
     4681 gtaacaggtg tcgttgtgga cgtttttcag accttcctcc accacggtgc gggaggtgga
     4741 ttccagcagc actacatgat acccgaactg ttccagaatg cggatgagca ggtggaagtg
     4801 aatggggagc atattgggtg caagaatggt ataggtcttt ttcatttccg gtgtaaactt
     4861 tgcgtatgcc atatgtatgg ttacctcctt ggaatgctcg ggttgcggtg catgcaggcg
     4921 tgttctgtgc ggaattgtca ctgtgggtgg ctgagggtgc tttcgtaaaa ccgaaggcat
     4981 aaggaaaaga ttttacgaag aaacccctcc accgcctaac ggcggtcccc ctcccctttc
     5041 aggggaggct ttttctatag tcatcgtact acagttttca ctggcaaaag attttacgcc
     5101 gtttccggct ctgtctgctt ctgtccctgt gtctgttcca ttgcggcgat caggctccgc
     5161 aggcggatct tgatggctcc cagattgctg atctcatcga tcttcagctg ggtgtacagc
     5221 ttgccgccgg tttccagaat ccgccgcact tcatctgtgg taatggcatc gataccgcag
     5281 ccaaaggata ccagctgtac cagctgcata tccggctgtg tggtgacata cttggcggca
     5341 cggtacattc tggagtgata cgtccactgg ttcagtgcct gcaattccgg tgtctgtgcc
     5401 agatgtgcca cgctgtcctc ggtgatgacc gccagcccca gactgccgat cagcttgtga
     5461 atgccgtggt tgatctccgg atcgatgtgg tacggtctgc ctgccagcac aatgatctgc
     5521 ctgccggctt cccgtgcctg ccggacgatc tcctcgccct cggcggtgat gtctttccgg
     5581 tactgttcca gtgctgcaaa gccgtgttcc agtgccgttt ccacctgctt tttcgtggca
     5641 tagcggctga atatggtgga cagcgtcttg accacatgcc cgcggcggtt caggtcgagg
     5701 aacgggtaca ggaaattgtt gtggttcagc ttttcgcagt tcgccatgag cagttccgga
     5761 taatacgcca ccaccggaca attgaagtgg ttgtcggaat cgccctcgtc gatgttgtaa
     5821 ctcatgcagg ggtagaagat gaagtccacg cctttttcca gcaggctctc aatgtgtccg
     5881 tgtgtcagct tcgccggata gcagaccgta tctgacggaa tggtgctctg tcccttgtag
     5941 taggtgccgc gggtgctctc ctcggagaac ttcaccgcga agcccagttc cgtgaagaac
     6001 gtgtgccaca ggggcatctg gtcgtagtag tgcagtgcca gcggcagtcc caccgtggct
     6061 ttcggctctg cgggctgatt ttcctgctcc agcgagagaa ttttccggta cttccactcg
     6121 tacagattcg gcagctgttc actctccttt ttgccggcac ccttttcaca gcggtttccg
     6181 gaaataaatc tgccgccgtt attgaaccgg atcacactca acgcacagtg tgcggtacag
     6241 cccttacagg tcgccgcctt ggcggtatag gagaagtcct gcaactgctg cggattcagc
     6301 agtgtgctgg tgccggtgct cttttcctga gcgtacagag ccgcaccgaa tgcccccatc
     6361 agaccggaaa ttgccggacg aatgacatcg tgaccgatct cccgttcgaa gcagcggagc
     6421 acggcatcat tgaggaaggt gccgccctgc accacgatat gctgtcccag ttcttccgga
     6481 ttgcgggcac gaatgacctt atagatcgcg ttcttgacaa tggagccgga cagaccggcg
     6541 gaaatatcct ctactgtcgc accgtccttc tgtgcctgct tgacgctgct gttcatgaac
     6601 acggtacagc gggagcccag atctacgggg tgttctgcga acagtcccag cttggagaac
     6661 tcggcaatgt catagcccag tgccacggca aacgcctgaa taaacgagcc gcagccggag
     6721 gaacatgcct cattcagcat gatgctgtcg atgctgtggt tcttgatctg gaagcacttg
     6781 atgtcctgtc cgccgatgtc gatgatgaaa tccacctccg gacagaagaa cgaagccgcc
     6841 ttgaagtgtg ccaccgtttc gacaatgccg aagtccacgc ccagtccggc acggatcagg
     6901 tcttctccat agccggtgac ggcactgccg caaatctgac agtccggact caggtcggcg
     6961 tacagggcgg acagctcccg ctgaatgcag tccagcggct gccccttatt ggaagcgtag
     7021 aactcgtaca gcagcttgtg gtcttccgac agcagcacga tcttggtggt ggtggagccg
     7081 gcatcgatgc ccagatacgc cttgccggca taggtgcggt agtcgccccg tgccacatca
     7141 tgggcggcgt ggcgttcctg aaaccgctgg tattcctccg gatagctgaa cagcggcttg
     7201 tctgccacca gagccgtctg agtcttggcg ttccggagca gttcttccag ttctgtcaag
     7261 gtgtatgtat ccgtcaggga cgatgcagac acggcactgc cgtatgccac aaaggttctg
     7321 ccgtcttcgg ggaacagggc gtgttcttcg tccagttcca gcgttctggt aaaggcgttc
     7381 cgcagccctt gcaggaacga cagcgggccg cccagaaaca gcacgtttcc ggcaatgcgt
     7441 ctgccctgtg ccagaccgga aaccgtctgg tccaccactg cctgaaagat gctggcagcg
     7501 atgtcctccc gccgtgctcc ctgattcagc aggggctgaa tgtcagactt ggcgaacacg
     7561 ccgcagcggg aagcaatggg atataccttt tcggctgcct gtgccatttt gtccagctga
     7621 tccacgctta cgtccagcag agtcgccatc tggtcgataa aggcaccggt accgccggca
     7681 cagctgccgt tcatacgctg ctccacgccg ccggtgagga agatgatctt ggcatcttcg
     7741 ccgcccagtt cgatcaccac atcggcttcg ggataccgcc gcttcaccgc cagaaacgcc
     7801 gcatgcactt cctgtacaaa gggcagcccc gccgcctgtg ccagacccag accggcagaa
     7861 ccggtgatgc tggcacggag ctggacgtcc ggatattcct gtgccagtga tgccagcttg
     7921 gtgcatacgg tttctctgac cttggagaaa tggcgttcat agctggaaga cagcacttcg
     7981 cccatctccg tcagcagaac cgtcttgacc gttgtagaac ccacgtcaat gcccagtgca
     8041 tacgggatct tttgtgtttc aagcatggat agctgctcct ttcctgtgac gcatccgcgt
     8101 cggctttcac gatttgtccg gcaggcattc ccgcgggaca gagatttcaa cttctggatt
     8161 ttagcttcta taaattttag cttttatata aaatgattgt acctgatttt ttcggggatt
     8221 gcaatggaaa attttccgtg cggcggggaa cccctccacc agcttcgctg attttcattc
     8281 cctttcaggg gaggctatta caggggaatt atgctccgat cacatcacgg ggcacacgtt
     8341 tggaaatgcc gcagacgatc tcgtacccga tcgttccgta cagggctgcc agttcgtcgg
     8401 cggtaatggt ttcttcgccg tccgtgccga acatggtgac aacatctccc ggcttgactt
     8461 cctccggcac gtcggtcacg tccagcatca gctgatccat gcagacccgt cccacgatgg
     8521 ggcagcgtct gccgtggagc agtgcgtccg cctgattgga cagcagccgt gcgtagccgt
     8581 cggcgtagcc aatggtcact gtggcaatgg tgcggggctt gtcggcagtg taggttctgc
     8641 cgtagctgat acacgtgccg gcatcgacct gcttcacgtg ggaaaccacg ctccgcaggg
     8701 acattaccgg ctgcaattcc ataggcacat gcacggggta gttgggttcc agtccgtaca
     8761 tgatgatgcc cacccgtgcc agcgtggctc tggggttggg gcgtgtcaca gtggctgcac
     8821 tgttcatgaa gtggagatgc ttcagggtgt gtcccagttc cgccagccgg tcatatacgg
     8881 caacaataaa gtcctcctgt gcctgcgtat acgccatgtc atctgcatct accgagtctg
     8941 ccacggcaaa gtgggtgtag atgccctcaa tggacaactt atccagttcg aacaggggca
     9001 gcagagcctt ggcacagtcc tccggcgtat tgctccggaa gccgatgcgt cccatgccgg
     9061 tgtccagctt gatgtggcag cgaaccggct ggtgggcgaa actttgcagc tgtcgggcat
     9121 attccagcga cagcacgccc tgaatgatgt tgttccgtgc cagttccggt gcaaactccg
     9181 gcggcgtata gcccagaatg aagatctctc cataggggca aagctgccgg acagaaaggg
     9241 cttcttccag attggaaacc gcaaaccagc ggactcccag ttcccagagc ttctggcaga
     9301 tcgcagcgtc gccgtgtcca taggcgttcg ccttgactac cgccataaac tgcgtctgtg
     9361 ccggcagacg gttctgcacg gtctgtacat tatgcgtcag tgcgttcagg tcgatctctg
     9421 cccacgcacg atagcgtttc tgctgttctt gttccatgat ccatcacgtt ccttttccgg
     9481 atacggctcc gccgcagtga tacggcaggc gggggtgcag gcagggcggc tgttacacag
     9541 tacgacaaat cagcaattgc aactcgccga tttttccatt gtcctgcttg caattttcta
     9601 tcaaatatgg tatgattagg ttgcattttc tatgccgggt gccgatgccg aaactggtcg
     9661 cggcaatgcc ggcataccct ttagtatagc acgaaacggg gaaaattgca agtcttagat
     9721 atgaatagat ttcgtttgtc cggcagggcg gtttttcgac aaaccccaca atcggacaag
     9781 cactttacga caaacacttt acagaaagga aaccaccatg tttacccaga tttttgacac
     9841 ccacagccac tacaccgaca gtgcatttga tgcagaccgg cacgaagttc tgtcggcact
     9901 gcccgaagcc ggcattgtcc acgctgtcct tgccggcacc acactggagg acagcaccgc
     9961 cggcattgcc cttgccgacc gctacgacta catctatgcg gcagtgggca ttcaccccga
    10021 aactgccgga cagcagcccg ccgattatct ggcacagctg gagcagctgg cgaagcaccc
    10081 gaaagttgtt gcgatcgggg agatcgggct ggactatcac tatgagggtt acgaccgcgg
    10141 gagccagagc gtgctgctgc gggaacagct ggatcttgcc aagcagctgg acttgccggt
    10201 catcattcac gcacgggact gcaccgagga ctatctgcgg atcttgcagg aatttcgtcc
    10261 ccgcggcgtg gtgcactgct tctccggcag tgcggaaact gcacgggaag tgctgaagct
    10321 gggcatgtat attgggttca ccggtgtgct gacgttcaag aatgcgaaaa aggcgttgaa
    10381 ggctctggcg gaagtgccgg aggataagct ggtgctggaa acggactgcc cctacatggc
    10441 accggtgcct tttcggggga aacgctgcga cagccgcatg atcccctaca ctgccgaagc
    10501 tgccgcactg gtacgcggca cggacaccca gacgattctg gaacagacct gccggaacgg
    10561 agaagcttta tttggaattc gggcggcggc ggcgagtcgc cgctgacctt acccccccaa
    10621 tctcaactgt gaaaagggta atgttacggc ggcagcagcc gcctgtatgc ccctccgggg
    10681 gatttttgtt gatgaaaatc tacgagattt tccatgctgt ctgtagggga cgatgcccac
    10741 atcatcccgc ctgtgtcaga aaagccctgc actacaaggg taatcacgcc ctgtgcaccg
    10801 tatgatgcac attgtgacac gtgcggtgca aaatgtgacg cagagtaaga gatagagata
    10861 gagtaagaga tagagtaaga gttagagttg tttctgggaa acaactgccg gagcagaaaa
    10921 ccgacatacc atttataata cttgacacgg gcagccgcct gtggttttgc cggctgtttc
    10981 ggctcggaca gccgcttttt ctggattcac gaaaagttta caaaataatc agaagaaaat
    11041 gtccgcaggg gcttgcaatt cttttgcaga catgctataa tatacaagcg tgattgcaat
    11101 agatatgcga tgaagcgaaa ggttgctggc ggtatgtcgg ggaattttcg tggagtatgt
    11161 ccgatttcaa accgggcgaa tcaggataac gacacagtct gtgtacacac tagttccgca
    11221 tggcgacagc tatgccggtt cgtgctgtac tgctgtttat gctggctcgg aaacatcttt
    11281 ggttttcgag tttttttata aacagatgcc gggaacaccc ggcggcgtgg aaagttcctg
    11341 atggagaagc gattctccag cccccaatag tacaaaggag gcgaaaagaa tggcagtcaa
    11401 cgaaaaaatc agaatcaaga tcaagggcta tgagcatgca actgtagatg cagcagcagc
    11461 taagatcgta gaggcagcaa agagaagtgg ttctcaggtt tcgggtccga tcccgctccc
    11521 gaccgataag gagatcatca caatcctgcg tgcaactcac aagtacaagg acagccgtga
    11581 gcagttcgag cgtcgtacac acaagcgtct gatcgacgtg gtacgtccga cacagaagac
    11641 caccgaggct ctgcagaagc tggaaattcc ggcaggcgta gacatcgtga tggaactcaa
    11701 gtaagaatct ctgattctga aagcggtgga aaaccaccgc acagcggcag gcatgccgcg
    11761 gtcatgttga cggatctgac tcgtcaacca ataaccaaca ggaggaaatc aaaatgcaga
    11821 aagcaattat cggcaagaag gtcggcatga ctcagatctt cgatgaagcc ggaaaagtga
    11881 ttccggtaac tgttgtagaa gccggcccgt gcgttgtcat tcagaagaag acaaccgaaa
    11941 acgacggcta tgaagcagtt cagctgggct tcggcgatgt aacgaccaag cacgttacca
    12001 agccgatgca gggtcacttc gcaaaggcag acgttgctgc caagaagacg ctgaaggaat
    12061 ttcgtctggc agactgtgca gccctgaacg taggcgatgt cgtaaaggca gacaccttcg
    12121 cagtaggcga cagtgttgat gtcagcggta ccagcaaggg taaaggtttt gcaggtgcga
    12181 tcaagcgtca caatcagcac agactgaagg aaactcacgg tactggtccg gttcaccgtc
    12241 aggctggttc catgggtgca tgctcctctc cgtcccgtat ctacaagggc aagggcatgg
    12301 caggtcacat gggtgctgaa aaggttaccg tacagaatct ggaaatcgta aaggttgaca
    12361 gtgaaaataa cctgattgcc atcaagggtg cgatcccggg catcaagggc ggaattgttg
    12421 tcatcactga cagcgtgaaa gcgtaaggaa ggaggaaaca tcaatggcaa agattgcagt
    12481 attggatatg gctggcaagc aggtatctga gagagaactt tcggatgcag ttttcgcagt
    12541 gactcctaac gaggcagtga tgcatgcaat ggtcgtaaat tatctggcaa atcagcgtca
    12601 gggtacacag tccactctga ctcgtactga ggttgccggc ggcggcagaa agccgtggag
    12661 acagaagggt acaggtcacg caagacaggg ttccatccgt gctccgcagt ggacacacgg
    12721 cggcgttgca ctgggcccga agccccgtga ctatcgttac gctctgaaca agaaggcaag
    12781 aagacttgct atgaagtctg cactttccac aaaggttctg gatcagaaca tgatcgttct
    12841 ggacagcctg aaggtggaag aattcaagac caagaccatc gttgcaatgc tcaaggcact
    12901 gaacgtagag ggcaaggctc tgctggttac cgctgaggct gaccagaagg tcgttaagag
    12961 tgcagcaaat atcgcaggcg tgaagacagc agctgtcaac accctgaatg tatatgacat
    13021 cctcaactat gacaagttca tcgttgcaga ggatgctgtc gcaaaaatcg aggaggtgta
    13081 tgcaaagtga aagcagcaca ggatattatt ctgactccgg tcatcaccga acgcagcgtc
    13141 gacgatatgg ctgagagcaa gtacactttc aaggttgcaa aggacgccaa caaggttgag
    13201 attgcaaagg cagcagaaga actgtttcag gtacaggttc tgaaggtaaa caccgtaaac
    13261 tgcaccggtc acaccaagcg tatgggcaga agcgtcggca agcgtccgga cttcaagaag
    13321 gctgtcatca ccatcaatcc ggagccgact ccggacaagg acggcaagaa gcgtaagggt
    13381 accatcgaat tcttcgacgg tatgttctaa gttacggcaa cgatcgtaac aagtatggga
    13441 gtaatgctcc cgcaaatctg gcattttatg aggagtgaaa aagaatgggc attaagacnn
    13501 nnnnnnnnga gaagagcctg ctggctccga agaataaagc tgccggccgc aacagctacg
    13561 gcagaatcac cgttcgccac agaggcggcg gaaaccgcag aaaatatcgt gtcatcgatt
    13621 tcaaacgcag caaggacggc atgaatgcaa ctgttctgac tctggaatac gatccgaaca
    13681 gaagtgcttt catcgctctg gttcagtatg aagacggcga aaagcgttac attctggcag
    13741 ctgacggtct gaaggtaggc gacactgttc gttccggttc tgatgcagac atcaagccgg
    13801 gcaacgcact gaagctggtt gacattccgg taggtacttt cattcatgcg attgaactgt
    13861 atccgggcaa gggtgctcag ctggtccgca gtgcaggcaa catggcacag ctgatgggca
    13921 aggaaggtgc gttcgcactg gttcgtctgc cctccggcga aatgagaaaa gtgccgatcg
    13981 agtgccgtgc ttctatcggt caggtttcta acgttgacca cgaaaacgta cactatggta
    14041 aggctggccg catgagaaac aagggctgga gaccgactgt cagaggttct gttatgaacc
    14101 cgaacgatca cccgcacggt ggtggtgaag gtaaatctcc gatcggtcgt ccgggacctg
    14161 ttactccgtg gggtaagccg gctctgggtt ataagacccg tgcaaagcac aaggcatccg
    14221 ataagtatat tgtaaaacgc cgcaacggta gataagggaa aggaggaaca cactcatgag
    14281 cagaagtatc aaaaagggac cttacgtcca ggaagtcctc ctcaagcgtg tacaggcaat
    14341 gaacgaagcc ggcgagaaga aagtgctcaa gacatggagc cgttcttcta ccattttccc
    14401 ggatttcgta ggccacacca tcgctgtgca tgacggccgc aagcacgtgc cggtatatgt
    14461 cacagaagac atggtaggac acaaactggg tgaattcgca ccgacaagaa cctttaaggg
    14521 tcacgctggt tccaagacat ccaataacgg aaagtaagcg gaaggaggag atcacatgga
    14581 agcaagagca tatctgagaa atgcccgcat ttcacctcgc aaggtacaga tcgttctgga
    14641 tctgattcgt aacaagcctg tggatatcgc actggcaact ctggacctca ctccgaaggc
    14701 tgccagcccg ctgctggaaa agcttttgaa gtccgcaatt gcaaatgcag aaaataatca
    14761 cagcatgaat aaggataatc tttatgttgc agagtgcttc gttaccgcag gtccgatcat
    14821 gaagcggatc atgccgagag cacagggcag agcctatcgc attttgaaga gaacttcaca
    14881 tattacgatt gttctgaaag aagaagagta aaatccagta ggaggttgag ttatgggcca
    14941 gaaggttaat ccgcacggtc tccgtgtcgg cgtcattaaa gattgggatt cccgctggtt
    15001 tgcgaataag tctgacttcg gcgatacgct ggtagaagat tacaacgttc gtaagttcat
    15061 caagaagaac ctgtatgcag caggtgttcc gaagatcgag atcgagcgtt tcgcagataa
    15121 ggttagaatc cacattcact gtgcaaagcc gggcgttgtt atcggccgcg gcggtgcaga
    15181 gatcgaaaag ctgcgtgcac agctggaaaa aatgctgaac aagcaggttt atgtcaacat
    15241 cgtagaagta aagcagccgg atatggatgc acagctggta gctgaaaaga tcgcactgga
    15301 tctggagaat cgtatctcct tccgccgtgc tatgaagcag tctatcggca gaaccatgcg
    15361 tctgggtgca aagggtatca agacaaaggt ttccggcaga ctgggcggtg cagaaatcgc
    15421 tcgttccgaa agctatcacg aaggtaccat tccgctgcag accattcgtg ctgacattga
    15481 ctatggtttc gcagaagccc acaccactta cggccgtatc ggcgtaaagg tttggatcta
    15541 taagggcgaa gttctcaagg gtgacgctgc taaggctgcc gctcagagag aaaaggtaga
    15601 gagaaagtcc cgggacaaca accgcggagg cagagatgac cgccgccgtc gtgacaaccg
    15661 gaacggtgac cgcagaaatg gcggcaattt ccgccgcgat gctagaagag aaggaggtaa
    15721 ccagtaatgc tgcttcctaa gagagtaaaa taccgtcgcg tacacagagg acgtatgacg
    15781 ggtaaggcaa ccagaggtaa tttcgtaagc aatggtgagt tcggtatcca ggcactggaa
    15841 ccggcatgga tcacctcaaa ccagatcgaa gcagcccgta tcgctatgac tcgttacatc
    15901 aagcgtggcg gtcaggtttg gatcaagatc ttcccggata agcccgtaac taagaagccg
    15961 ctgggtactc gaatgggttc cggtaagggt gctccggagt actgggtagc tgttgttaag
    16021 ccgggcagag ttctgttcga aattgctggc gtaccggaag aaaccgcacg cgaagcaatg
    16081 cgtctggcaa tgcataagct gccgatcaag tgtaagtttg taaaggctga aaaaggaggc
    16141 gagcagtaat gaaagcaacc gagatcaaaa atatgccggt agatgagctg aacgcaaagc
    16201 tggacagcct gaaggaagag ctctttgcac ttcgtttcca gctcgcagca aatcagcttg
    16261 aaaatacagc tcgcattaag gctgttaaaa aggacattgc tcgtgtaaag acagcactgc
    16321 gtctgtctgc acagcagtaa gtaggagggt tttactgtgg ctgagagaaa tttgagaaaa
    16381 accagagtcg gcatggttgt cagcgataag atggataaga ccgtcgttgt tgcaatcatc
    16441 gacaatgtaa aacacccgct gtataagaag attgtcaaga gaaccgttcg cctgaaggca
    16501 cacgacgaaa agaacgagtg cagagtcggc gaccgcgttg aagtgatgga gacccgcccg
    16561 ctgtccaagg acaagagatg gcgtgtgatc gagatcatcg aaaaggcaaa gtaagtcgaa
    16621 aacatctata gagtacaaac agaacggttt ccgataaggt cggaagcgat tctgaaagga
    16681 ggaactcgct gtgattcaga tgcagtctta tatgaaggtt gcggataaca ccggtgcgaa
    16741 agaactgatg tgcatcagag ttctcggcgg cactcgcaga agatatgcca atatcggcga
    16801 tgtcgtagtc gcatctgtta agaaagcaac acccggcggc gtggtaaaga agggcgatgt
    16861 ggtcaaggcc gtaatcgttc gttctaagaa gggccttcgc agagaagacg gaacctatat
    16921 ccgcttcgat gagaacgctg ctgtaattat tcgtgatgac aaaaacccga agggcactcg
    16981 tatctttggg ccggtagcaa gagagctccg tgataaggat tatatgaaga tcctgagcct
    17041 cgctccggaa gtactgtaag gcggaggtgt caagatgagt aaggtacacg taaaaacagg
    17101 cgacaccgtt atcctgctga ctggtaagga cgaatatctg tacaacgata aggacaacaa
    17161 gtccaagggc agaaagaccg gtaaggtcgt agctgtcagc ccgaaggaag gcaaagttat
    17221 cgttgaaggc ttcaacgtcg taaccaagca cgtaaagccg acccgcatgg gtcaggtcgg
    17281 cggcattgtg gaaaccgaag gtgcactgta cgcatctaag gtacagctgg tttgcccgaa
    17341 gtgcggtcag ccgaccagag ttggtcatgt gatcgaagac ggcaagaagc tgcgtgtctg
    17401 caagaagtgc ggcaaatctt ttgagtaaag gagaaacgat atgtctacat tgaaagatta
    17461 ctacaatagc accgttgctc ctgcaatgat gaagaaattc aactatgaaa atgtcatgca
    17521 gatcccgaag ctcgacaagg ttgttgtaaa cgtagcagcc ggcgaagcaa aggaaaatgc
    17581 aaaggtgatc gatgcgatca tgacagatct ggcagcaatc accggtcaga agccggtcgt
    17641 ttgccgtgca aagaagtctg tcgcaaactt caagctgcgt gagggcatgc cgatcggcgt
    17701 aaaagtgacc ctgcgtggcg aaaagatgta tgagttcgta tacaagctgt ttaatgtcag
    17761 cttcccccgt gtacgtgact tccgcggcat caacggcaac tccttcgacg gtaagggcaa
    17821 ctactctacc ggtatcaagg aacagctgat cttcccggaa atcgagtacg ataagatcga
    17881 taaggtaaga ggtatggata tcaacttcat cacaacggcg cagaccgatg aggaagcaaa
    17941 agaactgctc tctctgctcg gtgcaccgtt cgcgaagtaa tcagggagga aacagtcatg
    18001 gcaaaaaaag caatgaaatt aaagcagcag agaactccca agttttccac tcgtgcttac
    18061 acccgttgta agatttgcgg aagaccccat tcttatctga gaaagtacgg catctgccgt
    18121 gtttgcttcc gcgagctggc tcatgagggc aagatcccgg gcgtaaagaa ggcaagctgg
    18181 taaaaaggag ttctacgaaa aggaggtcat tggcatgcag attacagata caatagcaga
    18241 cattctgaca agaattcgta atgcgagttc cgcacggcac gccacagttg acgttcccgc
    18301 ttccaatgtc aagaaggcaa ttacccagat cctgttggac gaaggctata tcaagggctt
    18361 tcaggtaatt gaggacggca agcagggcgt gatccgcatc acactgaagt atacagacaa
    18421 tcgttctccg gtcatcaccg gtctgcgtcg ggtttctaag ccgggtctgc gtatctattc
    18481 cagttgtgaa gatatgccga aggtacgcaa gggattgggt attgcaatcg tttctacctc
    18541 taagggcatt atgacagaca agaaggctcg tgagctgaac gtcggcggcg aagttttagc
    18601 attcgtttgg taaggaggac acaggattat gtcaagaata ggaaaaaaac cgatcagtgt
    18661 tccttccggc gttgaagtaa agtgcgacga aaatctggtt accgtaaagg gtgcaaaggg
    18721 cacactgtcc agacagttct ccaaggaact gggcatcgca gtagaaaacg gcgaagtgat
    18781 cgtaactcgt ccgactgatg acaaggaaca ccgttccctg cacggtctga ccagaacact
    18841 gattgcaaat atgatcgaag gcgtaaccaa cggttactcc aagaccctca tcatcgaggg
    18901 tgtcggctat cgtgccgcaa agaagggcaa ggacgttgtc atgaatctgg gttattctca
    18961 tcaggtcatc gtatctgaga atgacgacat caagctggac gttccgcagc cgaataccat
    19021 cattgtaagt ggtatcgaca agcagatggt cggccagttt gcagcagaag tacgtgaaaa
    19081 gcgtccgccg gagccttata agggcaaggg tatccactat gtcgacgagc gtatcatccg
    19141 caaggaaggt aaagccggca agggcaagaa gtaagcagaa aggagtaaac cgctatgatt
    19201 aaaaagatgg atagcaagaa agccagagca aaaagacacg caagagttcg gtcgaagatc
    19261 tccggtactc ctgagcgtcc ccgtctgagc gtttaccgtt ctacaaagca catctatgca
    19321 cagctgatcg atgatgtcaa tggtgtaaca ctggcttccg catccagcat ggataaggca
    19381 tttgaaggcg aaggcggcaa tgtcgatggt gctcgcaagg ttggcgaaat gatcgcaaag
    19441 cgtgcagcag aaaagaacat cacggatgtt gtattcgaca gaggcggcta tctgtaccac
    19501 ggcagagtca aggaactcgc tgagggtgca agagaaaacg gactgaagtt ctaaaacagg
    19561 ggaggtatta ctttggctaa aattgatccg cagggactgg aactggctga aaaggtcgtt
    19621 tccattaacc gagtatccaa gaccgtaaag ggcggccgta tcatgaagtt tgctgcactg
    19681 gttgtagtag gcgacggaaa cggtatcgtg ggcttcggtc tcggcaagtc cggcgaagta
    19741 ccggacgcaa tccgcaaggg catcgaagat gccaagaaga atctcattca gatcccgctg
    19801 aagggtacca gcattccgca cgaaatcgtc ggaaagttcg gtgcaggcga agttctgatg
    19861 aagccggctg ctcccggtac cggtgtgatc gccggcggtc cggttcgtgc agtcatcgaa
    19921 gttgcaggta tcaaggacat tcgtacaaag tccctgcgtt ccaacaaccc gtgcaacgtg
    19981 gtacgtgcaa ccatcaacgg tctggcttct tgcacaacag cagcagaagt tgctgaaaag
    20041 agaggcaagt ctgtgaaaga aatctacaaa taaggagggt gcgaaatggc aaagaatatc
    20101 aagctcgtaa aaagcctgat cggtcgcaag aaggatcaga ttgctacagc aaattcgctg
    20161 ggtctgaaga aggtcggcga tgtcagcgta cagcctgaaa atgaggcaac cctcggcaag
    20221 attcagaaaa tttctcacct cgtagaggta acagaagcat aagttaatat ctaaggaggt
    20281 gcacatagca tgaagcttca cgaattgtcc ccggctccgg gctccacaaa agaagtaaag
    20341 cgtatcggcc gtggtcatgg ttccggtaac ggtaagaccg caggtaaggg ccataagggt
    20401 cagaacgccc gcagcggcgg cggcgtacgc atcggttttg aaggcggtca gatgccgctg
    20461 accagacgta tcccgaaaag aggtttcaac aacatctttg caaaggaata cgcgatcgta
    20521 aacgtttccg atctgaacaa gttcacagag ggtactgtcg ttgatgcaga gctgctgaag
    20581 gcagctggtc tgatcaagaa gacctgcgac ggcgttaagg tactcggtga cggtgagctg
    20641 acaaccaagc tgactgtaaa ggctgcaaag ttcacaaaga gtgcagtcga aaaaatcgaa
    20701 aaggctggcg gaaaagcaga ggtgatgtaa cgtgttcaag acgttgagag atgcttggag
    20761 tattgcggag atccgcaaaa agctcctgta cacattactg atgctcctga tcttccgtct
    20821 tggcagtgcc gtagttgttc cgtttctgga tacctctacg gtaagcacct ggatggcttc
    20881 taacgcgacc ggcggtaact tcctcgaata tctggatatt atcactggtg gtgcgttgtc
    20941 gcaggcaacc gtattttctc tgtctatcac accatacatc aacgcttcca tcatcatgca
    21001 gctgctgaca tatgcactgc ctccgctgga gcgtatgcgt gatgagggtg aagaaggaca
    21061 gaaaaagctc aacaaaatca ccgctgccgt ttctttggca ctgtccgtat ttatgtcctt
    21121 tgcatattat ctgactctga agaatcagat gaatgcagtt gtggacggcg gttccaaggc
    21181 agccaacgtc tttatcgcat ttgtcatcat cctttccttt atcgcaggta cctccatcgt
    21241 cgtatggatg ggcaaccaga tcaacgataa gggtatcgga aacggtatct ccatgctcct
    21301 gttcgccggc attctcgcaa gatttccggt tatggcaggt acactgggag aactcaccaa
    21361 gaacgaaccc cagcgttact tcttcgaatc tctgggcatt ctgatcctgt tcatcgcaat
    21421 gatcgctttc attgtcttca tgaacgcttc cgaacgccgg atcccgatcc agtacgcaaa
    21481 acgtgttgtc ggcagaaagc agtacggcgg ccagtctaca cacattccgg tcaaggtctg
    21541 catgagcggc gttatgccga tcatcttcgc catgtccttt atgtccctgc ccagcacgat
    21601 cagcctgttc aagccggtat atgatgatcc caccggcttc tggcagaagt tctataacgg
    21661 atttatcaac ttctttaata caagaacttg gacctacgct ctgatctact tcattctgat
    21721 cattgcattc aactacttct atgtgtccat gcagtataat ccgatcgaaa tcgccaacaa
    21781 cctccgtcag agcaacggcg gcattccggg cattcgtccg ggtaagccga cttccgatta
    21841 tatccagcgt gttctctcca agatcactct ggtgggtgca ttcttcctcg gtatcatcgc
    21901 gatcttcccg atcgcactga actggatgga cccgaacctc agttctctgg caatgggtgg
    21961 taccaccatc ctgatcgttg tcagcgtagc acttgaaacc gtccgcacgc tggagtctga
    22021 aatgatgatg cgtcatcata agggcttcct cgaataagca aggaggcata ttccatgaat
    22081 ctgattctct taggtgcacc gggtgccggc aagggtactc aggccgaagt gatctcacag
    22141 gcactgtcga ttccgcagat ttccaccggc aatatgctgc gtgaggcagt ccgcaccggc
    22201 accgaatatg gtgtcaaggc aaaggctgcg atggacagcg gtgctctggt ttccgacgat
    22261 attgtcatcg ggatcctgaa ggaacgcatt gcacaggacg actgcaagaa tggcttcatt
    22321 ctggacggtt ttccccgcag cgtgccgcag gccgaggcac tggacgaaat gggcgtgaag
    22381 atcgacaagg ttctggagat cttcgttccc gacgaaacca ttaagcagcg tgtttccggc
    22441 agacgtgtct gcgagggatg cggtgcaaca tatcacattc agttcaagcc gtcaaaggtt
    22501 gagggtgtct gcgacaagtg cggtgccaag accatcatcc gcaaggatga ccagcccgaa
    22561 accgtgctgg atcgtctggc agtgtaccac aagaccactg aaccgctgaa ggcgtattac
    22621 gaaaagcagg gcaagctgga aaccgtgatc ggacaggaag aagttgcgga taccacggct
    22681 ctggtattga aggctgtggg cgtaaaatga gtgtgaccat taaatccaaa tctgatatac
    22741 agaaattgcg ggaagcaggc agaatcgctg caaatgctct ggtacatgcc ggcaaggtaa
    22801 tgcaccccgg catgacaaca aagcagctgg acaaatccat tcacgatttc atcgtgggct
    22861 gcggtgcggt tcctaccttt ttgggatacg gcggttttcc ggcaagtgcc tgcatttcga
    22921 tcaacgacga gatcattcac ggtatcccca gtcacaaacg cgtgatccgc gagggcgaca
    22981 tcgtcagcgt ggacgtgggt gcaactttta acggattcgt cggcgacacg gcatatacat
    23041 tcgccgtggg cgacatttcg gaagaagcgg cagacctgct gcgtgtgaca gaagccagcc
    23101 tttacgctgc gatcgatgcg gcaaaggtcg gggcacgcct cggcgatgtg tcccataccg
    23161 tggaagaata ttgcggttcc cgtgggtacg gtattgtacg ggaatactgc ggacacggaa
    23221 tcggccgtga gatgcacgag gatccggagg tgccgaatta cggcaagccc ggccacggca
    23281 tgcggctgat gcccggcatg acgttctgca ttgaaccgat gatcaacgga aaaggcgatg
    23341 ccgtccgtgt gctcggtgac ggctggaccg tcgtgacccg cagcggctct cttgcggcac
    23401 atttcgaaca tgaaatcgca gtgacgcagg acggaccggt gattctcacc cgaccgtaac
    23461 ggaggtttcc gaatggaact gacaaagggt ctggtggtcc gtgcactcgc cggacgggat
    23521 atggggaatt ttttcgtggt gctgtcggtg gacggcggct atgccctcat cgcagacggc
    23581 aaacggcgga agctcgcttc tccgaaacga aaaaatctcc gtcatctcca gcggacggat
    23641 cagttcgtgg aactggacgg tgtcacggat aaaaagctca gaaatgtgtt gaaacaatat
    23701 caggcgtgcg actaacgcgt ccacgccccg aagataccca ttggggaggt tatcaatttg
    23761 tcaaagcagg atgtcattga ggtagaaggc gtcgttctcg aatcattgcc caatgcaatg
    23821 tttaaggtgg agctgcaaaa caagcacacc attctggcac acgtttccgg taagctgcgg
    23881 atgaactaca ttcgtatcgt gccgggcgat aaggtgactg tcgagatgtc gccctatgat
    23941 ctgaccaagg gcagaatcac ttggagagcc aagtaattgt gcagataacg cacagggaaa
    24001 ccgtgttccg gtaaccggaa tgctgttttc tatcgtaaag tatgcagacg gcatatcggc
    24061 cggctgcaca gttgttccga gaaggaggac agttgcaatg aaagtaagac cttccgtaaa
    24121 gccgatttgc gaaaaatgca aggtgattaa gcgtaaaggc aaagtaatgg taatctgcga
    24181 aaatccgaag cataagcagc gtcagggcta agctcgggat tttttaacat ggaggtgcag
    24241 gaagaatggc aagaatagca ggtgtagacc ttccgaagaa taagcggatc gagatcggtc
    24301 tgacttatat ctacggaatc ggccgcagca cagcagatga gattctggca ggtaccggtg
    24361 tcaatccgga tgtccgcgtc aaggatctgt ccgaagaaga cgttgcaaag ctgcgtgact
    24421 atatcgacaa gcacatcaca gtagaaggcg accttcgccg tgaagttgct ctgaatatca
    24481 aaagactcgt tgaaatcggt tgctaccgtg gcgtgcgtca ccgcaagggc ctgccggtaa
    24541 gagggcagcg taccaagaca aacgcaagaa cccgcaaggg tccggtaaag accatcgcaa
    24601 acaagaagaa gtaagggagg gtgtttcgtt ttggcacagc agaaaggcaa aaaggttgtt
    24661 gttcgccgcc gcagagagcg taagaacatt gaaaacggtg ctgtacacat tcagtccaca
    24721 ttcaacaaca ccatcgtaac catcaccgac acacagggca acgccatttc ttgggcaagc
    24781 tctggcggac tgggcttccg cggctccaga aagtctactc cgttcgctgc acagacagct
    24841 gctgaaaccg cagcaaaggc agcaatggaa cacggtctga agtctgtaga agtatacgta
    24901 aagggtccgg gatccggccg tgaagctgcg atccgtgcac tgcagaccgt tggtctggaa
    24961 gtgaagatga tcaaggacgt tactccgatt ccccacaacg gatgccgtcc gccgaaaaga
    25021 cgtcgtgtct aatcacactt acaggaggtg taatatatca tggcagtaag cagagaaccg
    25081 attctcaaga gatgccgcta tctgggtatc agcccgatgg ttatgggtat ctccaagcag
    25141 tctaaccgta atccgggtgc cggaaaccgc agaaaggttt ctgaatacgg aatgcagctg
    25201 aaggaaaagc agaagctgaa gttcatctac ggtgtaggcg aaaagcagtt cttccacctg
    25261 ttcgaaatcg caagcaagat ggaaggcaag gctggtgaca acctgatcgc ttgtctggaa
    25321 tcccgtctgg acagcgttgt attccgcatg ggtctcgctt tgacccgcag agaagcaaga
    25381 cagctggtaa cacacggtca ctttaccgta aacggccagc atgtgaatat tccgtcttat
    25441 cgtgtcaagg aaggcgacgt agtcgctctg gcagaaggca gccgtgacag tgcaaagttc
    25501 aaggacgttg cagaacagac cgcagaccgt accgtaccgg tatggctgga accggataag
    25561 gaaaacttct ccgcaaaggt cgttcgtatg gcaaagccgg aagatctgga ctatgaggtt
    25621 gcagcacacc tgatcgtcga gttgtactcc aagtaatcat ttgcaattta cggaacatag
    25681 agaaggatca ctcccggggc gtatgcctca ggagtggtct gatgcaaggt cctgccgtac
    25741 caaggagcat ctgcggccgt ggtatgtcag gaacaaccac agacaaagct accaagcata
    25801 ggagggtgtt tttatgctgg agaagattga aaagccggaa ctcgacacag tggatctgcg
    25861 aagcgacgga aaatacggta agtttgtatt ggcaccgctg gaacgtggct atggtatcac
    25921 ccttggcaac agtctgcggc gtgtgctgct ctcctcattg cccggtgtgg ctgtgaattc
    25981 cgtgaagatc gatggcgtac accatgaatt gagtacgatt cccggtgtaa aagaggatgt
    26041 gacggaaatc attctcagcc tgaagggact caccgcaaag ctctacggcg acggaccgaa
    26101 aacggtctat atcgaagcag agggcgagtg cgaagttacc gccggcgaca tcaaaaccga
    26161 ctctgaagtg aatatcctcg acccgggtat gcatatcgca actctgggta agggtgcaaa
    26221 gctgtatatg gaaatcgtca ttgataaggg cagaggctac gtttctgcag agcgtaacaa
    26281 gcagatgatg aatacgccta tcgacgtgat cgcagtagac agcatctata cccctgttct
    26341 gaaagtcaac tatcaggtag aagacacccg tctgggtcag gtcacagact atgacaagct
    26401 cactatggaa gtgtggacag acggcaccat ctctgccaag gaagcaatct ctcaggcagc
    26461 caatctcctc aatgagcatc tccggctgtt catcgatctc tctgatgaag ccagcattgc
    26521 agaagtgctc gtagaaaagg acgataaggg taaggaaaag atcctggaaa tgactatcga
    26581 agaactggat ctttccgtgc ggtcattcaa ctgcttaaag cgtgccggaa tcaataccgt
    26641 ggacgatttg atcaacaagt cggaggaaga aatgctgaag gtccgtaacc tcggtaagaa
    26701 gtccttcgat gaggtgcggg aaaagctcca gtccctgggc tttgatctcg ctagtgagga
    26761 agactaagcg tctggtttcg atcagacgtt caacaagacc gagccttgca gaatcctgtc
    26821 cccgccgcgg gacggctctg cagctagaaa aaggagtgaa taacaatgcc gggtaccaga
    26881 aaactgggca gaccgaccga tcacagaaag gcgatgctgc gtggaatggt aacattcctg
    26941 ctggaaaatg gtcagatcga aacaactgtc actcgtgcca aggaagtacg tgcagttgct
    27001 gaaaagatga tcactatcgc aaagtcggat gatctgcatt caaagcgtca ggttatggct
    27061 tatgtaacca aggagagcgt tgcaaagaag ctctttgacg aaatcgcacc gaagtatgct
    27121 gaccgcaagg gcggctacac ccagatcgtg aagattggac ctcgccgcgg cgacgcagca
    27181 gagatggcaa tcatcaagct ggtataagtt gtgtaatagc cggcaccgcc gtgtgccgta
    27241 acagacaggc gaagcatcgt acaggtgctt cgcctgtttt gttgtgcggg gctgtccaga
    27301 tgcagtatat ttagggcaat accgcacaaa gagcgtctgg cgactttgca cgccatctgc
    27361 ttgcaaattt ctcgtcatat ttcacaaaaa tgctcgtgaa tacatcaagt attcactccg
    27421 ctttttgttt catctgacga aaaatttgac gacgcatctg tcgcacaatc tttgtgcggt
    27481 attgccttag cctcccttga aaagggtgac tccccacggg gtggggagat gcccgcaggg
    27541 cagaggggac cgctcagtac gagggggacc agcgaagctg gtggagggat tcttgaattg
    27601 cagattttca cagcaaaatc cttcagtcaa caacacggcg tgccacgtcc gcattcccca
    27661 aagtggaaaa cttctccaac agctttgtgg aaaaggcacg tcgcgtcaat ccggcacagt
    27721 tttcaacatt gtggaaaact atttcggatg acggcggagg tgttttccac aaaaaatcca
    27781 ccaaagagga aaacttgtgg aaaacgtttt ttcgcaaaaa attcccgttt ccggtggtct
    27841 gccggtgctg attccaaaaa aacgccggtt tttgtcgaaa acgcactgcg tttcttgtgg
    27901 aatactctaa aaatgaagtc tatattttgt acaattctac taaaaaatgc gtatctctct
    27961 ttacattttg cgagttttag agtataattt gaatgaaggt ttctgcctgc cctgcagtgc
    28021 aggaacatat aagcagaaaa gccgccagga tgcgggaaca ccgccttgat tttccggtgt
    28081 ccccttgccc gtatccggca ggttctgnnn nnnnnnngca aaatgcgagc cggctggtct
    28141 ggcgtattgg gatattatac aattataggt ttcctgattc gtcgagttta cacaaaaact
    28201 ttgcgtatct attgactttt ctgttgggat attctataat aagaataatt ggaacagcat
    28261 gaatcagaga agcgattcct gcggagattt cgaccaagat tttttcgact gcatgccgcc
    28321 ggcctggaac accggcaggg cgtgcggaca atcagaggag gataacacat gaaagcaaaa
    28381 cacacacttg ctggtgctct ggcacttgca atgcttgctt ctactgctgt ttcgtttcag
    28441 gtttcggctg ctgacacagc ggtgaccctg aagggtgcaa aggtagaggc agaggctggc
    28501 ggagaatttt ctgtagacgt ttctctggaa gacattccgt ctacgaaggt aaacgtcatg
    28561 gacttcgcag tgacctatga tcagactgtt ctgaacgtag acagcgtgaa gatcggcaag
    28621 tctgctgatg ttgatgtttc cggcgacagt accgccagcg atgctccggt atttgccacc
    28681 aacatcaaga acggcgagat cactgtcagc tggacgaccg cactggactc caattcctgg
    28741 atctctgagg acggtgtgat cctgaccatt accggtactg taaaggacgg cgtggcagac
    28801 ggcaccgtaa ctccgatcga cttcgcaccg gttacccgtg aaacttatga cggctccggc
    28861 aagaacaatt ccagcatggt gatcggctat gtatacggcg gcgattccgc tacctatacg
    28921 atcaacgcag aagctggctc cgtgaccatc ggcagcaagc agaccaccac tgtgaccact
    28981 actgaaggcg gcaaggatac cactgctacc gaagcgacta ctaccgctac tgagagcaaa
    29041 acagatgtca caaaggcaac taccacgatc tccggcaaca ttgttaccgg tacggtagca
    29101 ccgggcagcg tgctgtacgg tgatgtcaac tgtgactccc gtgtggatat tactgatgcc
    29161 gtactgctga acaaggcagc agctggcgtg gttactctga atgacacaca gcggacgaac
    29221 gcagactgca acggtgataa agaagttggc tcgcaggacg caaccacact gctgatgttc
    29281 ctcgtaaaca tcattaagac tctgccgtat tcagaataat ctgtttcacg gcaaccacaa
    29341 atttttcgta tatttaggag aagtgttccg agggggaatg cggctcctga tataggcaat
    29401 atttcattta cgtatttgtc ggcaataccg gcaaggaaag gaaaggaaaa catgaagaaa
    29461 cgaacaaaga aggttctgtc cttcggcgtt gcagctctga tggcagtttc tgcactgccg
    29521 ttcagtgcac tgtcggtttc ggcttcgatt cccacaaaac agaccgaaaa tggcggcaca
    29581 ccgtacaatt tcctcctcga ggagcaggga agcggcgtac agaccatcag catctctaag
    29641 gacgagatcg ctgctggcga tgttaccaag actctggacg ttttcatcga ttctgaagaa
    29701 tggcctgatg acaactacct gagctctgta aagctgaact gggttgcaac caaggacgct
    29761 accttcgcag acggcggtgc aattgcaaac ggtatctact atcagaacgt tttcaacgca
    29821 tctaaaaagg tcaaccagca tgatgtcact atgccgaacg gtctgggcac aatcgtaggc
    29881 actccgtata gcggtgcatt ctgcatgtcc ccgatgttcg aagaggatga agacctgcac
    29941 acctggagct acaacgacgc tcagagtaac ggcgtaatca gagaaattgc ttgtgacaag
    30001 atctttggtg ccaatctgat cgccaacggc gacaacaagg tcaagttcga atacgactac
    30061 tatgcaaatg cagacgatta tctggcagac aaggatgcag ataaggctac tcacaagtcc
    30121 cataaggtac aggaatgcga tgtcaagcag aaggaagacg gtacaccgta catcgaatat
    30181 tcctacatta atccgggcgg ggaagttgct gcaaactatg ttgaaaagac tgttacagta
    30241 gatctgccgt gctattccac cactcgtttc gtaaaggact ctgatggaaa tgactgcgtt
    30301 ccggacatca acaactacta ttcctggggc ttcattgctg cttctccgga tgaccgtaca
    30361 tactttgttg gtggaaagtc tactgacctg cgtttcactt ccttcgatgt tgttgttccg
    30421 aaggataccg cagaaggcac ctattatgta cagatctgca gccgcaatgc aaacaaggat
    30481 ctgatcccga tgccgtccgc tgctgatttc gacagcgtaa aggcagatca ggaaaactat
    30541 cgtgcaacag taggtccgct ggaagatatg tccactgcaa ttcagggtaa gaaggatgca
    30601 gctacttact ctctgccgca ggatccttcc aaggcaatcg taaagatcgt agttggcgac
    30661 ggtactgtaa cagataccac caccactgcc gcagaaacca caaccactga aaacagcacc
    30721 actgctgcaa caaccgctgc aactactgtt accaccgatg acaagagcga tgaattcgct
    30781 tggaagatcg gcgaagtagc ttgcgaaccg ggcgacaaga aggttaagct ggaagtaaca
    30841 cagacaaatg gtatcagcac cactggtatc gttggttcta tgatcgttcc ggaagcaacc
    30901 cgcaagatcc tgtctgttcc gagctcttac aagaagatct ccaactgctt caagatcggc
    30961 gatgcatatc cggcactgag ccagaccatg atgaatacac aggaatacag cgaaactggc
    31021 cgtctggtat tctccatgag ctccaacggc agcatgggcg ttgtttctgg taacactctg
    31081 gctgcgttca ccatggacgt tgctgataag gacaccgtta cagcagcagc taaggaagct
    31141 ggtctggaac tgaagagcga caagaacggt aagtactatc tgttcccggt aacctggatg
    31201 gaagacggcg tagacatcgg ctacagcgat gacggtaagg gcaacctgac accggttgaa
    31261 gttaagcgtt ttgactatcg tgacaccgaa gataacgatg ttcatacaca ggtgaagtac
    31321 atcgacggcg gcttcaaggt ttatgttgac aagagcactg atccggttga aaagaccatc
    31381 aagtgggaaa tcgacaaggt taactgcaag ccgagcgatt ctaaggtaaa gctgaacgta
    31441 acacagacca acggtatcga cactccgggt gttgttggtt ctctgctcgt tccggaagta
    31501 actcgtcaga tcctgtctat cccgagttct tacaagaaga tctccaactg cttcaaggtt
    31561 ggtaatgtat atccggcact gagccagact atgctgaata cacaggaata cagcgaaact
    31621 ggtcgtctgg tattctccat gagctccaac ggcagcatgg gcgttgtttc cggcgaaaca
    31681 ctggcaacat tcaccatgga cattgctgat ctggcaactg taaagtctgt tgcagaagca
    31741 aacaatctgg aactgaagtc tgatgaaaac ggttcttact acgagttccc ggtaaactgg
    31801 atggaagacg gcgtagacat cggctacagc gatgatggta agggcaacct gacaccggtt
    31861 gaggttaagc gttttgacta tcgtgatact gaaaacaacg atatccacac cagcgtagat
    31921 tacgtagcag gtgcatacaa ggtatacgtt gatgcagaaa ccactacaac tgacgcaact
    31981 accactaccg aagaaaccac aaccactact actaccacaa ctacagaagc taccaccgca
    32041 gcaaccacag ttgctaccac cgctgcaact acagttgcta caactgctgc aactacagct
    32101 gctacaacca cagttgctac aactgctgca accacagctg ctacaactgc tgcaactaca
    32161 gctgcaacca ctaccacaac agaagagcct gtaacccaga agatggtttg ggctctggat
    32221 aagctgacag taactccggg tcagggctcc accgatggtg acaacctgag ctatgcaatt
    32281 actgttcaga acgctctgaa gaccggtgcc ctgctgggta caattgatct gccggaagca
    32341 accaaggaac tgctccagtg gccgtatggt acaaccgaca tgcaggtcaa ggtattcaac
    32401 cgttactaca agaatctgag aaatacggta tatgaaaaga tgccgacatt ccagatcgac
    32461 ctgatgaact tctggaagga agaagcaaac accaagatcc acttcggtct gatgtgtgat
    32521 ggaaacactg atttcgtaga accggcttct gcaaacggca cactgtacag catgccgatc
    32581 gcaattccgg acgaagcaac cgtacaggca ctggctacta agtatggcat cgaatatgat
    32641 gaaacactgg gcggctatgt attccctgta aactgggctg aactgggcac cgacatctct
    32701 tactccaagg atggcaacgg caacgttgtt ccggtttcta tcgatcgttt cgctctgaca
    32761 aacagcagca acgtgaagta tgacttcgct gaaactgttg acctgcagga tggttacatc
    32821 tgtgtaaagg ttccgaaggc aactactacc actactacag aagcaaccac tacaacaact
    32881 actgaggtta ctaccgcagc aactactgca gctacaaccg cagcagtagc tacaaccgaa
    32941 ccaactactg cagctacaac cgcagcaact actgcagcta caaccgcagc aactactgca
    33001 gctacaaccg cagcaaccac tgcagctaca accgcagcaa ccactgcagc tacaaccgca
    33061 gcaactactg cagctactac cgtaacaaca actgctgaag ctgagctgaa gatcgaagca
    33121 aatccggacg caaacttcta cctgtctgtt gacggtcgtg cgttcaaggc tgctgatctc
    33181 gtgaagtctg caactgtaaa cggcgaaagc gttatggacg atctgacctt tggtgcagaa
    33241 agcccgaagg cactgttcga caaggaaggc aaggcatatg tagataccga actcgatatc
    33301 ctgtataagg gcgagaaggt tgctactgca aaggtttaca tcggtattaa gggcgacacc
    33361 gacctgtccg gtaaggtaga ggctaccgat atgtactaca gcagctacta cattgcaaga
    33421 cagggtgctg gtattaagga tgcaaagctt ctggacggca cagagcacgc acaggatgag
    33481 aatctggaga aactgtcctt cttcctgacg gatattgata ccgaaagcaa ggcaggagag
    33541 aattctgcag acggcaagct ggaagcaact gacatcttct accaggcata ctatgttgct
    33601 ctggttggtg ctggtcataa gagcactacc tgggacaatc cggtttgccc ggatctgaag
    33661 aacctgaagg gctctatgtg ggctgaataa tcagattccc tgatttcgaa cgcgtaagcg
    33721 tttctgacaa caaggctatg acagcataag aaaaacccga tgcgggagcg atcccgtgtc
    33781 gggttttttg tattagtatg atttgccttg acaaactgaa atggcgtgtg ctatactgat
    33841 ttgtaattgc ataaattatt gtaaaaagcc atgcctcagg cataaaaagg agaactgttt
    33901 tatgggaatt gtagaactgc tgctgacagc gatcggactg tcaatggacg catttgcagt
    33961 atcggtctgt aaggggctgg gcatgcggaa gatgcggtac gatcaggcgt tggtcatcgg
    34021 gttgtatttc ggcgtgtttc aggcactgat gccgctgttg ggctggctgc tgggtacatc
    34081 attttcccgt tatattcagg cgtttgacca ctggatcgca tttgtgctgc tggcattcat
    34141 cggcggaaaa atgctctggg acgtgttcca cgaaaaggaa gaatcggctg ctgaaaaaag
    34201 tgatgccagg ctggatcaca aggaactgtt catgctggca attgccacct ctatcgatgc
    34261 tctggcggtg gggatcgcgt ttgcctgtct ggacgtgcag atctggagtt ccgtttccat
    34321 cattggctgt gtcacgctgg tgctgtcgtt ctgcggtgtc tggatcggca atcgctttgg
    34381 caaccgcttt gagaaaaagg cggagatcgc cggcggcgtg gtgctgatcc tgatcggcgt
    34441 gaagattctg ctggatcatc tgattcagca tcaatgaggg tgatctcatt ccgagtatgg
    34501 acaaattata gagaattcca gaatttcccg aattcttctc agaaatgtgt ttatattttt
    34561 gtacgaattt gttacaatat atacatgaat atgtgggaaa cgggggcatc tgatgaaaca
    34621 aagaagtgta aaatccaaga aaaaaattta cagtcagatt tttgggatcg cagtgagcct
    34681 gcttttgtcg gtggcggtga tttcgtctgc tgtttttctg catgaaagcc attatttcaa
    34741 ctccagcgag agccatgtac tggaagtgct ccgcggaatg gacggcaaga gcgaggtgcg
    34801 gtcgtgggac gatgaccgga atctaaaacg gcgggaaacg ctgctgcaaa aaggctgtgc
    34861 cgacaatccg gacatgatcg gctggctggt gctgccgcag acgatgatcg actatccgct
    34921 catgggcggc acagacagcg atagatacac cgaccacaac tatgagggcg gctacgactt
    34981 gtacggcacc ccgtttctgg acagccggaa tgcggtggat ttcagcgact ttttttcgct
    35041 ggtgttcgga catgatatga acaatagtgc catgttcggg agtctggacg attttttgca
    35101 ggagtcggca tttgcggact tgtcagacgg tgtactgctg ctgcctgatg aacggtatcg
    35161 tctggaggtg actgccagca ttgcggcaac ctgccctgca caggaactga tagaccagat
    35221 catgacggca gatgccgcag agcagccgca gctgctggag cagctgctgg aaatggcggc
    35281 ggtgaaaaag gacgatgtta cagtgtccga agaagaccac tgggtcatgc tgtccacgct
    35341 ggactatgcc gatgaaaaga acgacaacgg cacaccgtct atgctgctgc tgaagctgag
    35401 aaccagtacg acataaaaca cacgggcagg gaaacctgcc ctgtttttgt ggaattgcag
    35461 gggagaattt tctcccattt tgcacgattg actttttcgc agaagtgtga tataatatcg
    35521 ttaaggaaac actgaataaa gcttgtgcgt aagatgcgta gtcagatttt tcggcagatt
    35581 gcctagaaat tccgcaggca tactgccgta tggcaaggga tttctgggca atatgacgga
    35641 aaatctgcaa gcagattgcg tacaaagccg tcaggcgggc tttattcagc gtttccttaa
    35701 tctgattgga tagaaggtca cgcgggaaca tgcccgcgtc attggaaacg cacattctcc
    35761 ggaatgattc acgaacaaca ctctatgcca tttcgcctgt ctgcacgtgt acgcagaccg
    35821 gaacggctgg gtgcaggtgc gggaattgat tctaggagga ttttttatgc ccaaaaacaa
    35881 agtacaagaa gtaattttta ccattctcat ggtgtttgtc atggtctacg cgatgatctg
    35941 ctataacatt gccatagaaa ccggcagcat gaccaacatg gtatttctcc acgcttttcg
    36001 ggaactgccg atcatgggac cgatcgcctt tctgctggat ttctttctgg taggcggtct
    36061 ggcaaaaaag gcggcgtttc gcatcgtcga tctcgaaaag gacaaccctt ttcatctggt
    36121 gctggcgatt tccgccgtgt cggtgctgct gatgtgcccg ttgatgagct tggcggcaac
    36181 gctgctgttc aagcatgccg gcgtgcaggt gattgcagtc tggtttgaaa ccactgcgaa
    36241 aaattttccc atggcactgc tgtggcagtt gtttttcgcc ggtccgctgg tgcggttcgt
    36301 gttccggcac ctgatagcat tggtgcagcc gcgtgaaaag gcggcagtta cagaaaaggt
    36361 ctgatatacg caagatcccc gctccactgt gccgcagttc tggcagagtg gaacggggat
    36421 tgttataggc ataactgaaa cggcaggcgg ctcattattc ttgagtaagt ctgctgttgc
    36481 cgtttttgat gcgatgtgaa atattttgtt tgacattttg tgcggcattg cctataatcc
    36541 aaaaatagta agaagcatca ccacaaaaag aaaaagaaat aaccgcccct agcgtccaaa
    36601 tctgtggcag ttatttcttt ttaacctaag cagtgggagc agaccgtcta ctggtgtgct
    36661 ctctttatca ttagtataca cgatttccgc cgaaatgtca agggcaatac cgcacaaaga
    36721 ttgtgcgaca gatgcgtcgt caaatttttc gtcagatgaa acaaaaagcg gagtgaatac
    36781 tggatgtatt cacgagcatt tttgtgaaat atgagagtct gtttcgtatc tgacgtatgc
    36841 agattgcgtc gtcagatttt ttgagagatg aagggaaaaa cggagaaaat cctttgtgga
    36901 ttttcgagta ttttcccgaa atatctcgga a
//
LOCUS       DAWBGR010000004        34795 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_505_length_34795_cov_4.606534, whole genome shotgun sequence.
ACCESSION   DAWBGR010000004 DAWBGR010000000
VERSION     DAWBGR010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 34795)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 34795)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..34795
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_505_length_34795_cov_4.606534"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         7257 a        10288 c         8217 g         9033 t
ORIGIN      
        1 taattggtat atttctatta taccatattg tctaacaaaa ggggagcatt tcaaattcct
       61 gcgatccgtt tccatgtatt ggggagttcc gagaatctgc cgatcagtct ttcagcatac
      121 cctgatcgga atcgtcagaa tccttctttt tcttcttgcc tgcacgcttt tcctttgctg
      181 cggcgatctg tgcttcctgc ttccgcttcg cattctctgt catggtcagg ttttcctgta
      241 ctgcccagtt cttcagacga atcttgttgc ggttgccggt agtttcgatg acgatattgt
      301 cctcgttgac ctgtacgacc agacctacga taccgccaat ggtaacgatc tcgtcgccga
      361 cctgcaagct gctctgcatt gccttggctt ccttatcctt tttcttctgc ggacgaatca
      421 ggataaaata catcactgca aacagcagca ccatgaaaat cagatagccc actgtgccga
      481 agccgcttcc gcttgcactt ttgccgctgc cgctttctgc gaatgcagtc acgctgcaaa
      541 ccgcacctgt cagtgctgcc gtgctggcag ccagtcccag tcttgcgaaa ttcttactca
      601 tgttcaaaag ccctttctcc gttgccgtgt gattttgcca taggggttca cgcttttccg
      661 cgtaatacct catcgatcga cacagcagca gtctttccgg cacccattgc cagaataacc
      721 gttgctgcac cggtaacggc atcaccgccg gcataaacct tgtccttggt ggtagccatt
      781 tcttcgttga ccactaaaca gccccaacga ttggtgtcca agccctttgt tgtcccagag
      841 atcagcggat tgggagatgt gccgatcgcc ataattacag tgtctacatc cagcacaaat
      901 tcgctgtccg gaatagccac cggtctgcgt ctgccgcttt cgtccggttc gcccagttcc
      961 atgcgaatgc acttcagacc gttgacacgg ccgttctcgt cgcccagaat ttccaccgga
     1021 ttgcacaggt tctggaactc cacgccctct tccttggcgt ggtgtacttc ttccagacgt
     1081 gccggcatct ctgcctcaga acgacggtat acaatgtaca catgttctgc acccatacgc
     1141 agtgcacttc tggctgcgtc cattgccacg ttaccgccgc cgacaactgc cacagcctta
     1201 gactgaatga ccggtgtcgc atacttcgga tcgtagccgt gcatcaagtt gatacgtgtc
     1261 agatactcat tggcagaaca tacgcccacc agaccttcgc cggggatatt catgaagcgg
     1321 ggcagacctg caccggaacc gataaagacc gcatcatagc cggattccat cagttcatcg
     1381 acagacagga tcttgccgat gaccatatcc ggctgaatgt ctacgccggc atctttcaaa
     1441 gactggatct cctgctgtac gatcgtctta ggcagacgga attccggaat gccgtacatc
     1501 aatacgccgc cggcaacctg aaatgcctcg aaaacagtta cctgatagcc cagccgtgcc
     1561 agatctccgg cacaggtcag accagccgga ccgccgccga caatggcaac ccgcttgccg
     1621 ttgggctctg ccgtttcctg cttgccggta tagccatgct ctctggcgta gtctgccacg
     1681 aagcgttcca gacgaccgat tgcaaccggt tcgcccttct tgccgcggac gcacttgccc
     1741 tcgcactgat tctcctgcgg acagacacgg ccgcagactg ccggcaaact gtttgtggta
     1801 cggataatat cataagcctg taagaaatca ccggcagcaa ctgctgcaat gaaatccgga
     1861 atcggcacgc ctaccggaca gccgctgacg cagggctttg ccttacagtg caggcagcgt
     1921 gttgcttctt cccgtgcctg ttcgggagta tagcccatag ccacttcctg aaaacatctt
     1981 gcacgttcct tggggtcgcg ttccggcatg ggaaccttat tcggagccat attgaacttt
     2041 ccagccataa tcactgtacc tccttccgaa acaggttgca ttcctgttcc tgccgcttat
     2101 aggtaccgtt tcgacgcatc agttcatcaa aatccacctg atgtccgtca aagtccggtc
     2161 cgtccacaca ggcgtacttg atttttccgc cgacagtcac gcgacagccg ccgcacatac
     2221 cggtgccgtc caccatgatc ggattaaggg aaacgatcgt cttgacaccg aacggctcgg
     2281 tagtcttaca gacaaatttc atcatgggaa ccggtccgat ggcaatgaca acgtcatact
     2341 tcttaccctg ttccagctgc tgctgcaaca cattggtaac aaagcccttt gtgccatagg
     2401 aaccgtcgtc tgtgcagaga tacaggttat cactgcatgc acggaactcg tcctccagaa
     2461 tgacgatgtc cttgttccgg aagccaacaa tggtatccac ttccagtccc agactgtgca
     2521 ggtgctttgc ctgcggatac gcaatggcac agccaacacc gccgccaatc acgcagaccg
     2581 acttggtgcc ctccggcaat tcggtgggaa cgcccagcgg acctgccaga tccagaatgg
     2641 aatctcctgc ttccagcaga gagaactgcc gtgtggtttc accggcgatc tggaaaatca
     2701 atgtgatcgt gccgccctcg cggtcataat ctgctacagt cagcggtaca cgttcaccaa
     2761 actcatccag ccggaaaatg ataaactgtc ccggcaggac tttttttgca atcaacggtg
     2821 cagcgatccg taccataacc actgcgtcgt tcagcttttt cttttctacg atttgaaaca
     2881 attctttcac ctcatgcaat cgcattggct ataacagcaa ttctgttaca atccgaatga
     2941 aacaagccga cagaccgccg gcttgttgtt tgatccatat tcagggaaac actgcataaa
     3001 ggcaacaccg cacaagttta tgcagcgttt ccttcgctgc cgatgcattc cggcagcagt
     3061 tgaatcttat tccgcagaac cgtcctctgt ggggtcatcg atcggcactt caaaagtgtc
     3121 gccgtccaga tcgctgtagt caaactccag catctcgccg cagcacggac acggcatttc
     3181 gcctttctcc agaatcgatt cgctcagtgc gatgacattg ttgcaggacg ggcagactgt
     3241 ctcatagagt gtctcatcat cgtcgtcgtc aaaatcgtca tcatcaaagt cgtcatcaca
     3301 ggaatagtat gcgtcatcat catcatcttc atcatagaca atgtcttcca gatcgcccag
     3361 atcctgatca aacagatcgc agacctctgt cagttcatct acctgtgtct gcaggtctgt
     3421 gacatacgca gtcatttcct gcatcacgtc cagcagctgt cccagtactt ttccttcttt
     3481 ggtagtggtc agatccagct ccatgccgtc taccatacca cgcaaatatg ccatcttatc
     3541 tgtcaggttc atttgaaata cctcctgttc agcacacggt gtgcattgca tgcagcaccg
     3601 aaaataatca catcatgcaa gagccgcaga ctttttgtac tgccttatgc tctttccatg
     3661 tattcgccgg ttctggtgtc gatacggatc atgtcgccct gattgatgaa cagcggcacc
     3721 ttgatctctg caccggtttc cagtgttgcc ggcttggttg cgtttgttgc agtatcgccc
     3781 ttgaagcccg gatctgtttc ggtaacttcc agttctacga agttgggcgg ctctacgccg
     3841 aatacgttgc ccttatagga caggaccttt acttccatgt tttccttcac gaactggaaa
     3901 gccggaccca gcttggactt gtcgatgggc tgctgttcat aagtttccat gtccatgaag
     3961 tagtacagat cgccgtcgct gtacagatac tgcatgtcct tccgttcaat gtaagcggtc
     4021 ggatacttat cggtcgggtt gaaagaacgt tctaccactg caccagtgat gacattctta
     4081 tacttggtgc ggacaaaagc ggcaccctta cccggcttaa cgtgctggaa ttcgataacc
     4141 tgtaccacgt tgccgtccat ttcgaatgtg ataccgtttc tgaaatcgcc tgctgaaatc
     4201 ataaattgaa acctccgttt tatttctagg gaatgggggt cgatcccttt gatacttccc
     4261 tatatttcat gcaacgacag gaaacgccct gcataagtgc agagaatccc ctcaaaaaac
     4321 attgcttaac tttattatac aacataattg cttgctttgc aagactttct gcaaaaaaat
     4381 ttacgtttcc actggaaaaa tctttcggca gcaccgcacc ggtcagccga tcatcaccag
     4441 cactgcgttt ccctgttctg cggcattgtc atagaatgcc tgcagatctg cgaagcactc
     4501 ctgtagttcc gcacgaatgt catcttcctc ctccggtctg tcccagatat ttggatagat
     4561 ctctgccgcc gcaaaatcgg acatctgcaa ggctgccaga cgtgcggcaa aatccacctc
     4621 gtgcagagcc tgtgcaattt ctttcacacg ccttgccgga ataccgccca cgtgcatctc
     4681 ctgtgtcaga agaaagtcac tgccgcatac agcctccgac agcgggtcat ggtctgcggt
     4741 atctgacagc gtatctccgg tcagcacaaa atgcagtgcg tcccacatct tatccaaatc
     4801 acacacttca gcacgcagta cgtcccacat cttatccaga tcacacgttt ccgcagtttc
     4861 gtcctctgtc agttcttcca cccgtttcag cagtgcccgt tcctccagct gccgcagctg
     4921 ctccagttct gcgtcggaaa tccggtaata gccggcatac atacccatag tacaattctc
     4981 cttttacagc accagcagct gcttcggtgc tttcgtcaga ttctcgcagc cattctcccg
     5041 aatgactaca aagtcctcga tacgaacgcc gaactcgccg gacaggtaaa tgcccggttc
     5101 cactgtgact acatttccgg cttccagttt ggtctgtgtc gccggcgaag cgttggggaa
     5161 ctcgtgaatt tccaagccaa cgccgtgccc cagcgaatgc ccgaaagcat cgccgtagcc
     5221 ggcttcgtca ataatgtccc gtgccagctt gtccatttct ctgccggtga tgcccggctt
     5281 tgccccgtca ataccggcag cctgtgcttt cagcacaatg ttatagacct gctgcatctt
     5341 ttcgctgggc tgtcccacgc agacggtacg ggtcatgtcg ctgtgatagc cgtctaccat
     5401 tgcaccgtaa tccatcagca caaagtcgcc gtcctgaata attcgctgtg cagagggtac
     5461 gccgtggggc atagaggtag cacttcccgc cagtgcaatg gtggggaacg attcaccctc
     5521 ggagccgtgc tccatcatgg tctggttcag gcggagcatc acatcccgtt ccgaaatgcc
     5581 agcccgcagg aaattacaga tatccgcaaa ggcgatctct gcaattcgct gtgcctttcg
     5641 aatcttgtcg atctcggctt ctgttttcac tgtgcggcac gcgaaaatgg cacggctcag
     5701 agcatcgctc tgatccagct gcacctgatt ccccagctgc tgtttcagct tggagaactg
     5761 gctcaaagtc atggtcatcg actccaccga cagggtgcgg gcgttgtgtt tccggagcag
     5821 ttcccgaatt tgttcataca ggtcgtgctg ttccagaacg gtgcagtttt tcacgcattc
     5881 ccgtgctttt tcaatatacc ggaaatcaat gatcagatac gccccgtcgc ggaagcagag
     5941 cactgttccc gccgaggact gcatgccggt gagataccgg cggttgacat cagactggat
     6001 caaagctgca tcggttcctg ccggcagctt ctgcatcagg gtttccatac gcttttgcat
     6061 catgacaagc catctccctt acatctctga aagtgcctgc aatgccagaa aatagccgtt
     6121 aatgccaaat ccggcaatgg ttccggcaca aaccggacta agcaccgagt gagaacggaa
     6181 ctcgtcccgt gcatagatat tgctgatatg tacttccacg cagggaatgc gaatatcgtg
     6241 aatggcatca tgaatggcat agctgtagtg ggtataagct cccgcattga tgattacgcc
     6301 gtcaaacgtg gtgcgtgccg catgcagctt atcaatgatc gctccctcgt ggttggactg
     6361 gaagatctca cactccagtc ccagtccctc tgccctgctc agaatcatac ggttcaggtc
     6421 ggcaaatgtg gtattgccgt aaacgcccgg ttcccgttca cccagcaggt tcaggttcgg
     6481 tccgtgaatg accagtactt tttgcatcat gtcagttcag ctccttgttt tcttcatata
     6541 tctttttcgg cagaaatggg atttccattc gccgcttgaa tcggaaaaat ctcgttttct
     6601 ccatttccat gggaaagaca aaaatgcagg gcacaccaaa cagatttgct cccagcacat
     6661 cggtgaagat ctgatctccc acagcagcca cttctttacg ggacagtccc attttctgca
     6721 ctgcctcccg atagcccttt gtcagcggct tttttccctc gcagacgaag tccagcccca
     6781 gtcgtttggc aaaaggttcc acacggggcg ggtggttgtt ggacaccaga cacagcgaga
     6841 tccccgccgc ctgcatgcct gccacccacg cctccacgcc ggcagccggc accggattgt
     6901 cgtgtgttgt cagcgtgttg tcaatatcca gcaatagtcc gcacacgccc agcttttcca
     6961 gcaactgggg cgtgatgtcg cacacactgc gtaatgcaat ttttgcacga aacagcattg
     7021 cttctcctcc tttcggcaaa tcttcgcgat gctccgcctt acagcgtgcc ttcagtgttt
     7081 ccttcattat acgtcttttt tcccgccgga aatgtcgaat ctccgtttct ttccacaaaa
     7141 aaacgggcac actcttgtca agtgtgcccg cctgctgtgt atcgcaatac tttttattaa
     7201 tacttgcgct tacgagcagc ttcagacttc ttcttacgct ttactgaagg cttttcgtaa
     7261 tgctctctct tacgaacttc agcgataacg ccgtccttcg cacaagatct cttaaaccgc
     7321 ttcagtgcgg tgtccaaaga ctcgttctta ccaacgcgaa cttctgccac ttacatttcc
     7381 ctccctttgc cgctacggca gaggctaatc ttccgtctaa ccataataca gccaaacttc
     7441 tgatttatac caccaacaat ccctttcggg aacatcgggt atcagcttaa cctttttcgc
     7501 tgcaaactat attttgcaat atgatttatt gtaccacata ttgcagaatt tgtcaagggt
     7561 tttttctgca aacttagaaa tttgttattt taccacaaaa ttcacaagtt tctttggtac
     7621 atatatttgc tttatgattg ttttccctgc ggtagcttcc attatttttg tatctgccgc
     7681 agcctgctcc agaacggctt ccttttccgc atctgccgga atattcattt tggtcttgac
     7741 cttgccgttg atctgtacca cgatctcgat catatcatcc acgcacagag cctcgtcata
     7801 agtcacccat tcctgttcgc tgagcacgcc ttcacagccg atcagctgat acaattcctc
     7861 ggaaacgtgc ggtgcaaacg gatacagcag taccagcaga tcgcggaatt cctttctggt
     7921 gatcgagcct gcatcgtaaa tgtcattcag cagtgtcatc atggcagcga tcgcggtatt
     7981 gaacttcatg gattcaatat cctcggaaac cttcttaatg gtcttgtgca tcttggaacg
     8041 cagtgcgtcg ctgtagctgt cgctgtctgt taccttatcc tgcaatgccc agatacggtc
     8101 tacaaaacgc ttgcagccct tgacgctgct gtcgctccac ggagcggcct tttcatagtc
     8161 gcccatgaac agcacgtaca gacgcagcac atctgcaccg tagacatcga caacatcatt
     8221 cgggtctacc acgttgcctc tggacttgga catcttttcg ccgtccggtc cgaggatcag
     8281 tccctgtgca gtacgctttg catagggctc cgaagtcggc accagatcca gatcgtacag
     8341 gaacttgtgc cagaaacggg agtagatcat atggcgagtc acgtgttcca taccgccgtt
     8401 gtaccagtca accggcatcc agtacttcag ggcttcctgc gatgcgaatt ccttgtcgtt
     8461 atggggatca cagtaacgga ggaagtacca cgaagaacct gcccactgag gcatagtgtc
     8521 ggtttcccgt cttgctgccg caccgcactt cgggcacttg cagttgacga acgattcgat
     8581 ctttgccagc gggctttcgc cgtccttgcc gggttcgaaa ttctctaccg gcggcagacg
     8641 cagcggcagc tggtcatagg gtaccggcac catgccgcac ttcgggcagt acacgatggg
     8701 gatcggttcg ccccagtaac gctgacggtt gaacgcccag tccttcatct tgtactgtac
     8761 gcccttttcg ccgcagccct tttctgccag cacttccagc atcttcgcaa tggcatcttt
     8821 cttgttggtg atgccgttga ggataccgga gttcaccatt tcaccgtcac cggtataggc
     8881 ttccttagag atgtcgccgc ccttgatgac ttcgataata tcgatgccga acttcttggc
     8941 gaaatcatag tcacgggtat cgtgtgccgg cactgccata atggcaccgg taccataacc
     9001 catcataaca tagtcggaaa tgtagatcgg aatctccttg ccggtaacag gattgatgcc
     9061 ggtcagtccg tcgatcttga caccagtctt atcctttacc agctgcgtcc gttcaaactc
     9121 gctctttttc gcacattccg ttttgtatgc gtccagttct gcaatattgg caatgctgtc
     9181 gcggtacttc tgaatgatcg ggtgctccgg agcaataacc ataaaggtga caccgtacag
     9241 ggtatccgga cgggtggtat agatccgcag cttttcgtct gcgtgttcct tgacagcgaa
     9301 gttgacaaat gcaccggtag acttgccgat ccagttctgc tgctggagtt tgacattcgg
     9361 caggtagtcc acttcttcca gaccctgcag cagcttgtct gcatactcgg tgatccgcag
     9421 ataccagact tccttggtct tctggacgat ctcgctgtga caaatgtcac acttgccgcc
     9481 ctgtgagtcc tcgttggaca ggactacctt acagctgggg cagtagttga ccagtgtctt
     9541 gtcgcggaat gccagtccct tttcgaacat cttcaggaag atccactgtg tccacttgta
     9601 gtatttttcg tcagtggtat cgatcactct cgaccagtcg aaagagaagc cgacacgctt
     9661 gagttgtccg gtgaatttga cgatattgtc atcggtgacc ttccgcgggt gtacgccggt
     9721 cttgatcgcg gtattctctg tcggcagacc ataagcatcg aagccgatag ggaacagcac
     9781 gttgtagccc tgcatgcggc gtttccggct gacaacttcc agaccggaat atgccttgat
     9841 atgtccgaca tgcatgccgt gaccggaggg atacgggaac tctaccagtg cgtagaactt
     9901 cggcttggtg tgatcctcgc tcgcacggaa cgagccgtgc tcttcccaat acttctgcca
     9961 ctttggttcg acagaggcaa aatcatattt gttgctcatg tatttatcat tcctttgaat
    10021 ataatttact taccgaaaaa tgccttgaca ttcttttcaa acaccagcac ggctgctgca
    10081 cccagatcca gcagtccttc cagcagatag atccactggc tacccagatt gctgatattg
    10141 cttcccacat gcatcaggaa catcagtgcc agaaaaacgg cgacaatgta attgatgtag
    10201 ggaacgcggt tcagcatcag caccaatgcc acaacgctta caatcagcat caccagattc
    10261 gacatgctga accccagaat caggttcaga gcacccttga tgatgagata cagtccgatc
    10321 ccgaataccc attttcttcc tgtggtttcc atgataccaa atcctttcct gttgtgcgat
    10381 atgctcttac gcaatgccgt ttggctttgt ccactgagaa atgtctaccg gttccccgtc
    10441 tgcccacaga cgttccagct ggtagaattc ccgtgtatcc ttgcagaaca cgtgtacgat
    10501 aatatcggca tagtccagca ccacccagtt ggagccgttt ccgcggccct cgcggtgcag
    10561 cggttctacg cctttttccg acagctgata ttccacttca tctgccagtg tccgtgtgtg
    10621 ggtggtactg gtaccattgg cgataatgaa gtaatcaccc agaatggtca ggtcatgcac
    10681 ccgaagcacc tgaatatctt cggcacgctt gctgtccagt gcttttacaa tgatttccaa
    10741 aatttcctgc tgcgtcatat ttgttccttt ctcattctta ttgtgctccg tcattgctgt
    10801 cgcccttgtt gatgtcgccg agattttcct gtgtgtcatc ataatctctt gccagatact
    10861 cgccttccgg caccagttcc acaatggtgc tttcctccgg atagatctcg ttctgatagg
    10921 ggcggaaata ctggttcagc agatccacag ttgcagactt gtgaatagaa tacaccgact
    10981 gggaagagcc gtcccccgga tagtaagttg ctccttctcc cggcaccata aacacgttga
    11041 cattgtccat agtcagacgc tgtgaggcaa agtctgccac catgctgatc tgttccagcg
    11101 acagatcggt agcgatccac tgattcttat agatcttctg catggcactc atcagttccg
    11161 tctggctcat gttcagcatt ttctccattg ctgctgcaag gaagatccgc tgtgccttaa
    11221 cgcgtccgat gtccccttcg gcatagccgt ggcggaaccg gacaaaccat tcggactgct
    11281 gaccgttcag ggtctgttct cctgccggca gaatcttatc tgcttcatag gtgatctgct
    11341 ccggcagcgt aatgggaatg ccgccgacag aatccacgat attggcaata tccacgcagt
    11401 tcagtttcac ataagcatcg acatagatgc agaagttttc ctgaatggca ttgaccacac
    11461 gctggatcgg tgttacgccg gtttcctgtc cgctgtgata aatgctgttg aactttgtgg
    11521 tggaataaga agcataatcc ggcatgtagg agtcacgggg aatttgcaga atgttcatgc
    11581 tgcctgctat caggtcgaac tgcaataccc agtctacatc agacaattcg ccgctttcgt
    11641 ccatgcccag aaacaagatg gtatactgtc cctcaatggt ctgctccaaa tccagtccgt
    11701 catttacatc cgaatccgga ttgcttttca ggttcagtgc cgcatcgtta gagtcaacct
    11761 ttgccagcgt accctcctta tctaccagcg gctgccacag tttccagtac ttcatgatcg
    11821 aaacctgctg ggtggtcaag gtcgttcctt ccagcttttt aaagttgatg atcggtgcat
    11881 tcaggaccat cagcaggatc agcacaacgc tgactacgat tgccgcaacc ataacagcaa
    11941 tatcccgcgg gctatttttc tttcgttttt tgggggcata gtagccgtgt tcatctgagt
    12001 agttttttct gctcatctgc aataatcatc cttcctgctg ttgcagctcc gttaaaactt
    12061 cgttgtaggc ttctactgtg tgatgcggga tcagtgcttc ccgccgcacc agctttatca
    12121 gggaatattt cagggcatat gccatgcctg cattgaggct ttctgctgcc ttttcccgca
    12181 tcacgtccac atcgggataa tcccgttccg cagagatcat atctgccaga tagacgatct
    12241 tttccagatt ggacatgccg gctctgccca cggtgtgaaa ccgtactgca tgcagcacat
    12301 cttcatcagt cacaccgaat ttcgtccgca gcaactccgc accggcgatg ccgtgccaga
    12361 ccttgaatgc cttgcgttct tccatgcaca cgtccataga ggactgcatc atcagcgtgt
    12421 actgtacgtc ttccggttct tccttgcaaa tatcgtgtac cagaccggcg aaacgggcgg
    12481 tttccacatc tgtgccgccg aatttctctg ccagcatcac actggcctcc gaaacatttt
    12541 tggaatggat atagcgtttt tgcgaaagac gggctttcag atactcgtca tattgctcca
    12601 gtgactccat gaaaaatgtg agctcctttc ctcgggaaca tcttatgcat ataaattatg
    12661 agatactata tattgtacta ctttttgcgg taaatagcaa gaaaaatctg catgattttt
    12721 tcgattcctg cgaatttttg tagaagatac cgtgcaaacc tcagcaggac acaaaaaaat
    12781 tttgccatat tgcaataaaa atgctctctg tgcctccagt tccgccatat ccccgtccat
    12841 gcgggacacc acgcccagtg ctgccatttc cagaatctcc tgccagcagt gccacttcgg
    12901 aaatgacagg aacatatcgc tgcccaccag cagcaccagt tctgcttcgg gataccggtt
    12961 ccggaacagc cgcacggtgt ccacggtata gctgacaccg gcacgttcca gttcgcagcc
    13021 gtctaccgtg caaaagggca gcgtttccgc cgccagacgg cacatcgcca gacggtctgc
    13081 accggaagcc atgtccggat tctgctgctt gaacggcgac acattggcgg ggatcagcac
    13141 cacctgatcc agccccagac tttccctggc agtctgtgcc agatgcaggt gcccgttgtg
    13201 aatggggtta aagctgccgc caaacaatcc gattcgctgc atcagaattt aatgaccggt
    13261 tctttcggat tccgcttgaa cagaaccagc cggctgccga tgacctgtac cacatcggat
    13321 tccgtctttt ccgacagttc ctctgcggct tcacgggcac tcagcggaca gttgtccagt
    13381 acgtggatct tgatgagttc ccgtttccgc agagcatcgc tgacctgctg caccagtgta
    13441 tcgctgatac cgccttttcc cacctgaaaa atggtttcgt atgtgctggc gatgccccgc
    13501 agggtcgccc gttgtttact tgtcaacatc aatttttctc cttaatcatt tttataagct
    13561 ttgaatttat aaagttttaa aaaaatcttc cagctgttcc aatgccttgc cccggtggct
    13621 gatctggttc ttctctgcat cagtcatttc tgccatggtc tgttcgccta cgcagaaaac
    13681 cggatcatag ccgaagccgt tttcccctct gggagaagtt ccgatcacac cctcgcaaac
    13741 gccggagaag aactgctcct cccctgccgc attcacatag cacagcacgc actggaaccg
    13801 tgcagtacgt ttttctgcgg gagtgtcttt cagcagttcc agcagctttt cgcaacgctg
    13861 ggcatcagtg gcattttcgc cggcaaaccg gtgcgaatat acccccggtg ctccgtccag
    13921 tgcatctacc agcagtccgc tgtcgtctgc aatgacggca cagtgcaggg cttcgtgaac
    13981 cgcatgggct ttcagtgccg cattctctgc aaaggtagtg ccggtttctt ctacttctgc
    14041 atcaaagccg gcttctttct gggaaactgc gtccagcccg aaaggggcaa ggatcgcacg
    14101 gatctcccgc agctttccgg cgttattcga tgcaatgaca atctttttca tagtgattcc
    14161 tccgtaggtt caaacagggc acggacaaaa tcctgtgcgt ggaacggctg gagatcgtcg
    14221 atcttttcgc caacgccgat cagcttcacc ggaatgccca gttcgttgcg aatggatacg
    14281 atgataccgc ccttggcagt accgtccagc ttggtcagca caatgccgga aatgtctgcc
    14341 acttcctgaa acagccgtgc ctgattgact gcgttctgtc cggtggtagc gtccagcacc
    14401 agcaggattt cccgatgaca gccctctgcc ttggtttcga taatgcggtt gatcttcgcc
    14461 agctcctgca tcagattttt cttgttgtgc agccgtccgg cggtgtcgca gatcagcacg
    14521 tcacagcccc gtgccttggc ggcatcaatg gcatcataca ccaccgatgc agggtcagag
    14581 ccttctgcat gcttgacgat gtccacgccg gcacgctgtg tccagacttc cagctggtca
    14641 attgccgcag cacggaatgt atctgccgct gccaccagca cctttttgcc ctcgcctttc
    14701 agctggtggc aaagcttgcc gatcgtggtc gtctttccgg caccgttgac accaattacc
    14761 atgacaatgg taggctgggt gggatattcc agtgtctggt cctcacccag catctcaccg
    14821 atgatctcct gaatcatgcc catgatctcc tgcggatcct tgacaccccg ttccttgacg
    14881 cgtttccgca gggtttcaca gatctgtaca gaagtttccg gtcccagatc gctggtaatc
    14941 atggtttctt ccaactcgtc aaacaggtcg tcgtcgatct tggtaaagcc gcccagcacc
    15001 tgctgcatac gggaaaccat tgcgtccttg gtctttcgca gaccgtcccg aatcttcgca
    15061 aaaaagccct ttttcttttc gggttctgct tcttccggct gagattcgcc ctcgggttct
    15121 atttctgctt ccggctcgga ttcttctgtc agttctgatt ctgtttctgc ttcctgttcg
    15181 ggttcttcaa caggctctac ttcttccggt tcttctgctt cgggttcagc ttcttcctcc
    15241 ggttcggttt cttcctccgg ttcggtttct tcctccggtt cggtttcttc ctccggttcg
    15301 gcttcttcct ccggttcggc ttcttcctcc ggttctgcct tctcttcatt tttcaaaatg
    15361 ccggcagctt ccagagccgc ctgccgtgcc aacgcaattg cagcctgatg ctgttcagat
    15421 tcctccacaa cttcctgtgc ggcaaggcga gccttttcga tctcgtcctg ctcctgtgcc
    15481 gccaagtcat tgagggcagc cgcggcaatg tcttcttctg cttctgcatc agccgtttcc
    15541 gcagtctgtt cctccggttc caataccgtt tctttctctg cctgctgctt gttccgccgc
    15601 cagaacggta cgcctttttc cttgttttta tttttctttc cgaataagcc catcttacac
    15661 cgatcctttc cttgaaagca tagacatcgc cgtacaaagc ggactttgtg cggtattgcc
    15721 ttattccgtt ttctcgagtt cttcctgttc cattcgcagc agacgggaaa taccgtcctc
    15781 ctgcatggtg acaccgtaga gtacactggc ttcttccatg gcactccggc ggtgcgtcac
    15841 cagaatgaac tgggtggtat ctgtaaagtt ttgcagatac tgtgcatagc ggcggacgtt
    15901 tccctcgtcc agtgccgcat cgatctcgtc cagaatgcag aacggcgacg gccgcagccg
    15961 cagaatggcg aagtaaatgg cgatcgccac aaaggactgc tccccgccgg aaagcgagat
    16021 cagattcttg atgacttttc ccggcggtgc gaccttgatc tcaatgcctg attccagtac
    16081 atgttccgga tcagtcagtt ccagccgtgc ttctccgccg ccgaacaggt ctttgaaaat
    16141 ttcctgaaag ttccggtcaa tgacagaaaa gctttccgaa aagattcgct gcatctcctc
    16201 tgtcagagag gtgataaggt cttccagttc ccgcttggca gtttccgcgt cctgcatctg
    16261 ctccgacaga aactgatacc gcttggaaac ctctgcatat tcctcgatcg ccgccacgtt
    16321 taccgtgccc agcccccgaa tctgtgcttt caggctttgc agttcccgct gtgccgtctg
    16381 taggtcttcc agcggctgtg cctgtgcctg tgcttcggag agggaaagtt cgtactgctc
    16441 cagcagctgc cggatcacgc cgtcataatc gttctgcacg gaaatttttc gttcctccag
    16501 ccggctgatc tcgccggaaa accgctcccg cacggcgttg gtttcctgca aaccgctttg
    16561 cagttcccga atctgctgct cctgtgcatc atgcacctgc tgccagtgcc ggatctctgc
    16621 ctgcccgtcg gtacggctgt tctgcatctg ttccagcctg gcttgattct cggtgatctc
    16681 cgtctgcttc tgacggatca gatcttcctg cacggcaata tccttgtcga tctgcaccag
    16741 tttttccgcc atggaatcct gctggtcgct ctgctgctgc atttccttcg ccagcgtgtc
    16801 catgtccttt tccagttcca tcatgccgat cttcagctgt gaacggcgtt ctgccagcct
    16861 ggactgctcc tgttccagag ccgtccgctg gtcggcttcc tgtgacagca tttcctgtcc
    16921 cttttgaatc gccttttcca cttcctccgc ttcttctgcg gcggcagtgt gccggtcaac
    16981 ggtttcctgc aagcgttcct gcaacgccgc ctgttgtccg gcagcttctt cggcacgctc
    17041 ggtggtctgt ttccggagat actgttcctt ttccagctgt gcagctgcgg caacctgttc
    17101 agattccgcc tgccggcact gctgctgcaa cgcttccatt tgctgctgta actgtgcaga
    17161 ctgctgctgt gccttttgca gggcagcaga agccgcctgt tcctgctgtt ccagttcctg
    17221 tatctcgccg gaaagagccg tcagttctgc ccgtctggtc agcatgccgc cggcacgcat
    17281 ggcagaaccg ccggtgaaag aaccgccggc attgatgacc tgcccgtcca gcgtgacgat
    17341 acggaaccgg tactgataag ctttcgccag agccgtcgcc gtgtccagat cttccgccac
    17401 gacgattcgc cccagcagga actgtaccac gccggaaaac cgttcctcat accgcaccag
    17461 attgcacgcc agatccacaa agccctcgtg ctgttccaga ccggtttcgt gcagcgtctt
    17521 tccccgcacg gaagtcagcg gcaggaatgt ggcacgtccg gcgtgcttct gctgcaaaaa
    17581 gcggatccac tgcttggcag cgttttcgtt ttccacgatc agattctgca tggcaccgcc
    17641 caatgcggtt tccagtgcca cgccgtattt ctggtcggtg gtaatcagct gtgccaccgt
    17701 gccgactacg ccgcggggcc tgccgccgtc tgcccgcagg actgccttga cgctgccgcc
    17761 aaagccctcc atgtttcgtt ccatatcctg tagcagtctt tgccgctgcc gtttttcccg
    17821 cagggcgaat gccgcctgct cctgttgctt cttgccggct tccagtgcct gcactgcatg
    17881 agcgtagcgt ttctgaaaac cgtcccgctg attctgcacc tgttggaact gttcctgtgc
    17941 ctgctgtgcc tgttctctgg cggcatcata cgcggcattc tgctccgtgc atgcctgccg
    18001 gagtgcactc tgccgcatgc cctgttctgc cagctgagct ttcagttctt cctgctgtcc
    18061 ggcagcggtc tgctgcaaga cacgcagttc actctgccgc accagcagat tttgcaatac
    18121 ctggtttgcc tgttcgtatt cttgtccccg ccgtgcagcc tgctgtttca gggcgttcca
    18181 gttctgttcg gtctgttccg cttctacctg aatcgcttcc cgcttctgcc gcagctgcac
    18241 cacctgttca gacagggact ccatgcggtt ttccatgctg cgggactgct gagccgtttc
    18301 ctgtttctgc tgccgcaggc tctcctgcat ctgccggctg tggagaatgt cgttttcaca
    18361 gactgccacc gcagatttca gctgggtggt ttcttcttct gcctgctgca tctgctggcg
    18421 aatttcctcc attttcagcg tgctttcctg catacgccga tagccgtcct gcacctgtgc
    18481 atctgtccgc tggatctccc gttcggcatt ctggtattcc gcctgtgcct gtaacaagcc
    18541 gtcagacagc cccgtcagct tttcccgcag agcacccagc cgccgcaccc agagggaaac
    18601 ctccagctgt ttctgtttgc ctgccagttc cacgtactgt ttcgcctttt ccgcctgctt
    18661 tcgcagcggt tccacgcggg attccagttc agaaacaata tcctgcaaac gcaccagatt
    18721 ttcctgtgcc gcgtccagct tccgctgtgc ctcctgtttc ttatagcgaa acttggaaac
    18781 ccccgcagcc tcctcgaaaa tgtcccgccg gtctgcactc ttggcactga cgatttctgc
    18841 aatgcgtccc tgtccgataa tggcatagcc gtcacggccc atgccggtat ccatgaacag
    18901 ttccgtcacg tccttcagcc ggacgttctt gccgttgatg cggtactcac tgtcaccgct
    18961 gcgatacagc tttcgggtaa cactgactac agcaccatag gcttctccca actcgccggc
    19021 ttcgttgctg atctccagcg taactgccgc aaagcccatg gcctttctgg tttttgtgcc
    19081 ggaaaaaata acgtcctcca ttttgttgcc gcggagcgtc ttggtggact gttcccccag
    19141 cacccaacgc acagcgtcgc cgatattgct cttgccgctt ccgttcggtc ccaccacggc
    19201 ggtcagacct ttgtcaaagt gcagtttgat cttgtcaggg aacgacttga atccctgcag
    19261 ttccagggag cgtagataca tgtttgcatt tcacctcttt ttccggcggc gtatcccgca
    19321 ttctttacga ttgcagcaca cgcactgcat agattttccc gccgaagtcc gacagcggca
    19381 ctgtctgttc tttgacttgc agcggatcca tacagagata tgttccgtca gcactgccca
    19441 ccagcagcac atagtgatag ctgccggact tgggcatctt cacccgtaca atggggtatc
    19501 gtcccgctgc cagttcctga tccagttcgc cgtcctgcaa ttccgccgcg tcctgtttcc
    19561 ggacagaaac accggcagtc tgttccagca tctcccacag aagattgccc gcactgtcgt
    19621 acacaccgtg ttccgaaaag aactgattca cctctccggc atcggcattt tccggcagac
    19681 cgtccacaga gatctgctgc atctgcaacg ccgctgctac gcagcaggtc agacagccgg
    19741 aatccgccat gtgatagaca gaatcgccca gcgtgtccgt tttccatcgg tcatctttct
    19801 gccggaaaaa gaccacctgt tccgccgcgt tttcctgtgc ggtttccacc acagcaggag
    19861 ctgccccgcg gtgccggttc caaaaaaatg caccagctgc ggcacccagc agcaaaaccg
    19921 ctgccagcac tgccgccaga caacgtctgg tatgttttgt cctcatcgtt gattcctttc
    19981 cgaaaagatc tcatagacct gctgccctgc cagaagcgtt cccgcagcaa cggcagcatt
    20041 ttcccgtaca cgaagcgaaa cgccctgctc cgccagtgcc ttccggttgc aaccgtgctg
    20101 ccccagcacc ttgctcatgg tacggggagc cgtttccaca aagagttccg tttttccaca
    20161 gtctgctgcc gccgctttca gctgccgcag atacagacgg gattccgcca gttctccata
    20221 ggcagggtgg aaataccccg ccagcagatg ttcccgcagg gacggcgtat catgcagccc
    20281 cagccggatc acccgcacgc cggcatggtg acagcggcac agggcatctg cacaatagtc
    20341 cagcacttct tcctgtgaca actgcggata gccgccgcgg aaataatact ctgccagctt
    20401 ggtgccctcc agcactgccg tgggatagat tcgcagggtt tccggctgac acgccagaag
    20461 ctgttccagc gtgtcatatt cgtcctgcaa tgtgctgctg tataagccca ccatttgctg
    20521 caaaccaagc gaaaaacccc gttcccgcag catggcactt gcctgccgga ctgcctgtgc
    20581 atcgtgtccc cgttcattct gatacagcac acggtcacac atgctctgtg ctcccagttc
    20641 caccgcagtg acatgatagc gttccagccg ttccagaact gcactgtcta ccgcgtccgg
    20701 tcgggtggaa atacggatac cggaaaacat gccttctccc agaaacggct gtgccgcctc
    20761 cagcagggca atctgatacg ccggttccac cgctgtgaag ctgccgccga aaaaagcgat
    20821 ctccgccgat tccagcgacc cgctgcgttc cagcattgcc tgacaggtct gtgtcacctc
    20881 ttccggcgtg ggagcctgca ctgtgccgct gattgttcgc tggtcgcaga agctgcactg
    20941 gtgcggacag ccccgatggg gaatgaagaa cgccagtgtc ttatgctgtg ccatagccca
    21001 tcagggacag tgcttccttg gcagccagct gttccgcagc ctttttgctg tgtcccacgc
    21061 ctttgccgat gacattggaa ttcaggcaga caatcacttc gaacgccttg tcatgatcgg
    21121 gaccgcattc gctgacaagc agatactcca ccttttcttc cggattcttc tgcacaactt
    21181 cctgtagccg tgtcttatag tcgtggaagc cggcaggctg ctgtctttct gcaaccggat
    21241 tcttcggaat aaaacgcatg atatgccgct ttgccgcatc catgccgccg tccagaaaga
    21301 tcgccgcaat gacagcttca aacgcgtctg ccagaatcga cgaacgggaa cgtccgccgg
    21361 tgtgctcctc acccttgccc agcaggagga attcgcccag atgaatctct ctggcgaaca
    21421 catgcagtgc cttttcacat accattgagg cacggagttt ggtcagttcg ccctccggca
    21481 ggtcgggata cttgtgaaac agaaattccg aaaccacgat cgacagcaca ctgtccccca
    21541 gaaattccag acgctcgttg ctgttgcggg tcttatgctt ttcgttagca taggacgaat
    21601 gggacagtgc ttccagcagc aggtgcttgt ccttaaagtg gtaatggata tacgtttcaa
    21661 accttcctaa atctacatac tctgtatgtt ccatgattgc tttccctcta tttctttacc
    21721 ggctttccgg tattttctgc aatggtctgt accacatttc cggcaacgca gtccttcgcc
    21781 tgccggatcg cattgaaaaa tgctgtgccg tcggaactac cgtgtgcctt gatgaccggc
    21841 ttctgtacgc ccagcatcac tgcaccgcct acggctttgt agtccatttt tttggtgagt
    21901 gccttgatct tcggcaggat cagcagtgcc gccagctttc cggcaacgcc tgcgaacagg
    21961 ttgcctttca tctggtgtgc cataaacttg cccatgccct cgtacagctt cagtgccaca
    22021 ttgccggtaa agccgtctgt gactaccaca tctgctgcac cggcaggcag ttcccgtgct
    22081 tcgatattgc cgatgaaatt caccggagcc tgtgccagct ggacatagga ttccagttcc
    22141 agttcccgtc ccttggtttc ctcggctccc acattcagca gagccacgcg gggcttgtcc
    22201 acgtgcatga cattctgcat ataggcactg cccatgatcg caaactgcac cagcatttcc
    22261 ggacggcagt cgttattggc accgccgtcg atcagcagaa acggtgtcgt ttccgtgggg
    22321 agcaccggag ccactgccgc acgcttgatg ccggcgatcc gcttcaccag aaatgtggct
    22381 cccaccaaca gggcaccggt actgccggca cagacaaagg catcaccctt gccgtcgtgg
    22441 agtgcctgca tgcccactgc catggaagta tccgcatgct gtttcagcag ggacgtgggt
    22501 tcctcgtgaa tgtcgaacac tgccggagcg tgcagaattt ccatgccgtc cagcggcagc
    22561 ctgtttttct cggcacaggc acgaattttc tgctcgtccc ccaccagcgt cacagtcaca
    22621 cccagttcct gcaccgcccg tgcaccgccg cgaatcactt ccagcggggc gttgtccccg
    22681 ccgaatgcgt cgattaaaat attcaaaatc tatgatctcc cttgtgttat gacaataccg
    22741 tgcaaagccg tcagacaggc tgtttgcggt atcgccttat tgttccgtgg tctgcaggta
    22801 ctgttccagt gccgccatgc cgcgatccgc gtatgcctga tacttttcct gtttaccgcg
    22861 gattcgctcc ggcagatccg gcagaatgcc catattggca cccatcggct ggaaattctc
    22921 cgtgggagca ctgctgatgt atgccgccag tgctcccatc atagtttccc gcgggagaat
    22981 cagcggcggc ttgccctgca gctgtcttgc cagattcttt ccggcaagaa tgccgcttgc
    23041 cgccgattcc atatagccct ccacaccggt catctgtccg gcgaagtaca gcccgtcctg
    23101 cccgcggaag tgatagtccg catctagcag gcggggactg tccagaaatg aattccggtg
    23161 catcacgcca tagcggtgaa attccgcatg ctccagtccg gggatcaagg agaacacccg
    23221 cttctgttcc ggaaatttca gattggtctg aaatcccacc agattataca tggtgccctc
    23281 ctgattttcc cgccgcagct gcaccactgc ccacgggcgg tgaccggtgc ggggatctgt
    23341 cagccccacc ggcttcagcg gtccgaaccg catggtgtcc atgccgcggg aagccagcac
    23401 ctcaatgggc atacagccct catacacccg aaaggcatcc ttgtcgcagt cgtgcagcgg
    23461 tgcacgttct gccccgatca gtgcctgata aaattcctca tactctgcct tgttcatggg
    23521 acagttgata tagtctgcat cgcctctgcc atagcgagcc gcaaagaaca ctttctcctt
    23581 gtccagactc tcaaagctga cgatcggtgc agcggcatca aagaaactca gccgtgtgcc
    23641 gcagcgtttt tcaatatccg ccgccagtgc cccctcggtg agaggtcctg tggcaaccac
    23701 tgtgggcgta tccggaattt ccgtgaccac agccgtttcc agcgtgatgt tggggtgatt
    23761 ccgaatcgct tcggtggcaa ggtcggagaa ctgtttccgg tctaccgcca gtgccccgcc
    23821 ggcagcaact gccgactttc tggcacaccg catcagcagc gagccaagac gagtcatttc
    23881 cgccttgagc agtcctgccg cagaattcag gcggtcggct ttcagcgagt tggagcagac
    23941 cagttccgca agcccctcat agtgatgagc cggtgtatac tgcaccggtt tcatctccag
    24001 aagccgcacg gaaaagccgg cttctgccag ctgccacgcc gcttcacagc ctgccagtcc
    24061 gccgccgatc acagtcactt ctgccattta agcacctcct cccacaggac gggaatagcc
    24121 gcatcccggc ttttcgcaaa ccagtgtgcc ggacttgccg cccttctgga acagcgtaca
    24181 gccgcactgg ggacactttt cctcggtagg cacattccat gtcatgaagt tgcagtccgg
    24241 ataggcgttg cagccgtaaa agcttctgcc acgcttggac tttttcagaa tgatcttatt
    24301 gccgcacagc gggcaggagc cggcagtttc ctgtacgatc ttcttggtgt tcttgcactc
    24361 cggaaaacca gggcatgcca gaaatctgcc gtagcgtccg atcttgatga ccatgtttct
    24421 gccgcactga tcgcagacaa tgtctgtttc ctcgtccggc accttgacac gtttgccctc
    24481 cattttctcc tcggcagttt ccagcgtctt ggagaagtcg ccgtagaaat cttccagtgt
    24541 gtccacccag cttttcttgc cgcgttccac atcgtccaga ctgttttcca tatcggcggt
    24601 gaacttcacg tccagaatgt tggggaaatg ttctttcatc agttccgttg tcacttcgcc
    24661 cagaacggtc ggcttcagct gtttgccctc tcgctccaca tacagatgtg tggtaatggt
    24721 ggtaatggta ggagcatatg tactcggtct gccgatgccg ttttcttcca gtgccttgat
    24781 gagcgttgct tcggtatagc ggggcggagc ctgtgtgaaa tgctggttgc cctccagcga
    24841 ttttaccttg atctccatgc cgttttccag ttccggcagg ttcttgttct cgctcttgtc
    24901 gcccttggca tcgtcataca gcaccgtgaa gccgtcgaac ttcaccgtgg agccggttgc
    24961 cttgaacata cagttgtcgg cttcaatatc cacggaaacc gtatccagca gagcatctgc
    25021 catctggctg gcgataaagc gttcccagat cagacggtac agcttatact gatccgcagt
    25081 caggctggat tttacccgtt ccggtgtcat gtccggcata gacggacgaa tggcttcgtg
    25141 agcgtcctgt gcgtttttct tggacttata atttcggcgt tccggcggca ggtactctgc
    25201 accgtatttt ccggtcacat agtccgcggc tgcctgctgt gcctctgcgg aaatccgcag
    25261 actatcagta cgcatgtagg tgatcagacc cactgccccc agtccgtcga tgtccacgcc
    25321 ttcgtacagt tcctgtgcca ctttcatggt acgcttggac tgaaagccca gccggtagga
    25381 agcgtcctgc tggagggtag acgtgataaa cggcggtgcg gggtgccgct tggtgacacg
    25441 ctttttcacg ttctgcacag aatagctggc attttgcagc cgttccagca aagcatctgc
    25501 ggtggctttg tccggaatct ccgttttgtc cacccgctta ccgtccacct ttaccagctt
    25561 ggcggcaaac accttgcggg acggcggggc ggtaaatttc gcatcgatcg accagtattc
    25621 ctgcggaaca aatgcccgaa tctcattttc ccgatcgact accagacgca cggcaaccga
    25681 ctgcacacga ccggcggaca gtccgcggcg gacttttttc cacaggaacg ggctgagctt
    25741 ataacccacc agacggtcca gaatccggcg tgcctgctgg gcattgacca gatccagatc
    25801 gatcttgtgg ggattgctca tacctgcctg aatgccgctg cgggtgatct cgttgaattc
    25861 cacccgatcg tcggcgttca tatccaaatg cagaagctgt gcgatatgcc atgaaatggc
    25921 ttctccctct ctatccgggt ccgtcgccag atagacatgg tcgcacttct tcgcatgttc
    25981 tttcaacttt ttaatgacat cttccttgcc cttcatgtcc gtgtactggg gctggaaacc
    26041 attttccaca tcaacaccca gcttggactt gggcagatca cggatatgcc ccattgaggc
    26101 aatgacatca tagccttttc ccaaatattt ctgaatggtt ttcgcctttg ccggcgattc
    26161 cacgatcact aaattcgaca tattcctctc gttctctctg ctggctgttt ctcacagctg
    26221 ttttttctca agatttgtcc gtagcgaaca gacacaggac agcctcaacg catgcaccgg
    26281 aattgttttc ccggcaggcg ttccaatttc ccgtacagtt ccagttcggt cagcgtggtg
    26341 gtcagttcct ccatggaaac ctccagttcc gctgcgatct gatccatgtg catcacgtcc
    26401 tgcttttgca gcagggacag gatctctcct gccagccctt ccagctcttc ctccggcatg
    26461 ggatccagta ccggatcggg ttctgcttct gccggcggtt ccggaacgga aacctgtggt
    26521 ttcttcgccg gtgtctttgg cttcgtttcc gcctccccca gcgtcatcac agaactttcc
    26581 gatttgtccc gatcctctcc gtacagtgcc gccgccccga tcatatgggc gtggctggtg
    26641 taatactcat agacaatgtc ccgactgtca aacacgggaa ttgcaccgtc ccgaagatat
    26701 tttatcacac ccatataccg tttgtcaaat atgtccgccg gtggaataca gaaaacatct
    26761 ctgccctgct ccatggcgtt ctccgccgta atcagtgcac cgcttcgaac cggtgcctgt
    26821 atcacgaaag tgcccatgct caaaccggac agcacgcggt tccgcagcgg aaaatttgcc
    26881 ggtgccggag aagttcccgg cagaaactcc gacaagatca agccgtgctt ggcgatttca
    26941 tatttcaagt ttacatggga tttcggatac gccacgtcca gcccgcagcc catcagggca
    27001 atgctgggct tgtccgcctc cagagcacac aaattggcgg tgatgtcaat accggcggca
    27061 tagcccgtca ccggcacaaa gcccatctgc accagatcat gacaaatggt tttctccacc
    27121 cgtaaactgt actccgccgg attgcgagtt cccacaatag tcagcagcag gtgattttgc
    27181 agcagggagc tgtccccctg ataaaacaac aataccggtg gattcacaat ggacaaaagc
    27241 tgttcgggat atgccgcatc tccataccag agaatggaca gacgcagctt ttcacagcgt
    27301 tccagcattt cgtccacttt atcctcaccg gtctgacgaa ttcgccgcag agtgtgctcc
    27361 ggcagatgca gatcggcatc tgtttccggt ttccgcaaga tctccacggc tttctgcgga
    27421 gaatgatagc ggtgcagtgc cttccagatt cggggatttc ctgcaccgaa tatcatgacc
    27481 agccacagca gacagcgtat ttcttccatc tcactgccct ccgttctggt cctgtgccgt
    27541 tctctgcact tcccacagaa tgatgccggc tgccatagca gcattaaggg attcgatcgt
    27601 cccgtgcatg ggaatcgtca cacggcgatc acaggcagcc gctaccgttt ccggcaatcc
    27661 gttgccctcg ttgccgatca cgatcgccgt gccgccggaa aaacggcatt gccccaccag
    27721 ttccgccgca ccggaaacca cagccgcaca ggtttccaca cctgcacacc gacaagcttc
    27781 cagcacaggg gcaatatccg gcacgcaaca gagcggcaca cggaacaacg agcccatcgt
    27841 tgcccgcacc actttcggac tgtagatgtc acagctggca ctgtagatca cgccgtccag
    27901 ccccagtgcg tccgccgtcc gcaggatcat gccggcattg ccgggatcct gtagctgatg
    27961 cagcacggcg tagctgccgc ctgcgtggat caatgcggaa agtgccggtg gctggggcat
    28021 ctgacaaatg gcataaacac cctgtgaatg ttccgtctga gagagttccg ctgccaggct
    28081 atccgagagc tgtgcacaac gcacaccgtc ccacgctgtc ggcaagccct ctgcctgcca
    28141 gcgtgccaat ccgtcctccg tccagaacag ctgggtcaga gccgcaccat ttttcaccgc
    28201 gtccgacacc agccggaagc cctccagcac aaacagcccc gccttggtgc gttcttttcg
    28261 ggaacgtgcc agttttcgat attgttttat ggcggcattg tcgtgtgccg agattttcaa
    28321 cagtgtttcc atgtgctctt tccccctctt ttgcaagagc ctgtttcgta tccgtttctg
    28381 tgcagatgcg aaacaggctc tatggcaacg cagtgtttcc cttataatac aacaaaacac
    28441 gccccgtatc atttccagag cgtgtttcgc attcaaatcc ttacagagca gccttagcct
    28501 gttctacgat tgcagtgaag cctgctgcgt catgaattgc gatttcagac agcatcttgc
    28561 ggttcagggt gatgccagcc ttcttgcagc cgttcatgaa ctgggagtag ttgatgccgt
    28621 tcatcttgca tgcagcggaa atacgagtga tccacagctg acggaactct ctcttcttct
    28681 gctttctgcc gacgtaagca tagttaccgg acttcatgac agcctgtttt gccatcttga
    28741 agtgcttgct cttgctgccc cagtagccct ttgccagctt cagtgtctta tttcttctct
    28801 tgcgagtcat cattgcacct ttaatacgtg ccatagttat ttacctccgt tatttctcag
    28861 attccaatca aagcccgaat attcctgcgg cttagagata tggcatcagc tttttcagtg
    28921 ccggtgcgtt tgtgcagtca gcgataccaa cggtacgagc gattctcttt ctcttggtat
    28981 ccttctgtgt cagtctgtgt cttttgttgg tcttctgata ctttaccttt ccggaagcag
    29041 tcatcttaaa acgcttcttt gcaccggaat gagtcttggt cttgatcttt gccattgtga
    29101 attcctcctg tttacttcga tgccttcggt gaaatgaaca tgaccatgct gcggccttcc
    29161 attttcgcgg gcttgtcaac aacgccgact tccttgcacg cttcctggaa acgcttcaga
    29221 agttcttcgc cgatctgcgg gtgtgcaatt gcacgcttcc ggaaacggac ggaaaccttc
    29281 aacttgtcgc caccctgcag gaatttcttt gcctgattga ctttggtttc aaagtcgtgg
    29341 gtgtcgatgg cagaaaacat acggatctcc ttaatcgaaa cgaccttctg gtttttccgt
    29401 gcctcttttt cccgcttctg ctgttcaaag cagtacttgc cgtaatccat gatccggcat
    29461 accggcggtt ttgcacccgg tgcgatcttc accagatcca gatccttttc ataggcgatt
    29521 ttctgtgcat ctctcgcaga cattgttccg agtttttcac cgttctggtc gatgaccaga
    29581 atttctctgt cccgaatttc ctcgttgatc tgcagttcct gtttgctgat aagccagcac
    29641 ctccaaacat gttattttca aacaaaaaaa agcgtccact gttgggtgaa cactccttct
    29701 ccattcgtca aaaagcgtcc cttttccgca gaaaaaggtc gttatacacg agtattgacc
    29761 gtttcgactc tacgccaaac ggtgagaacg ggaatgctct tctttgttta cttttgttat
    29821 tttatcatac tcccgtcctg ttgtcaagca tctctccgaa aatttacaga acatctatat
    29881 ttcagaacca ctgtttcagc cattccacgc atttcagctt gactgcatag cgttcatggt
    29941 gctgtagcat gtcctgttcc gacttaccga atgcctcctg tgcagtttct tcgtccgccg
    30001 tgacttcgca ggcattgggc acgcagaggg acggatacat tacgcaccac cagttctgtc
    30061 ctgcggcttc gccgatggtg atccgcagag cctcatagtc tcctgccggc atggtccatt
    30121 ccccataagt gcggtcgtca aagggcattt ccaccagctg tgccttgact gcataggaat
    30181 atccctcatc ccacaccagc tgctgtgctg tctgctccag cgtttccaga tgctctgcta
    30241 cgagctgttc cgcatcggca gcattttccg gctgggaccg gaacagggct tctgtctgtg
    30301 caagcacctt gtcccgcacc ttgagtttca acgcctgatc gtcgatgctg tccgagttcg
    30361 ccagaatgtg cagccgcagc acattctgtt ccagcgagcc gtaactctgt gccgttcccg
    30421 ccagtgccga acagcagacc gttcctacca tacccaaaag caatgcccat tcccatttct
    30481 gcatctcact cacctcgatg agagtatgca ccggaatctg ttccgttata cttgcagagg
    30541 aaattttttc atggtataat aaaaacagca ccgcacaaag actgcctgtc ggctttatgc
    30601 agtgttgtcc aaaagaaata ccagtagcat cggaggaata cacatgaaag tttataccat
    30661 tatcggtggt gtcaacggcg taggaaaaag cagtttttcc ggtgtcctgc ggcagcagcg
    30721 actggacttg ggaaaaatca ttgatccaga tcatatcgcg gcaaaagagc actgcaacaa
    30781 actggaagcc ggcaggatcg ccattcagga aatggaaacc gcaattcaaa acggctggaa
    30841 cttcacgcag gaaaccacgc tttccggcag acgcacacta cagaccattc ttgcagctcg
    30901 acaaaaggac tatcagattc ggctgtatta tattggcatc agttccgccg aagaaagcct
    30961 ataccgcatt gccaaccgtg tccgcaaagg aggacacaac attgccgaag cagatgtacg
    31021 ccgccgatac gagaagcgat tcgcagacct ctccaaaatt ctgccctatt gtgacgaagc
    31081 ccattttttc gacaacgaaa acgggttcac agaagcggca atttatcaga acggctccct
    31141 gattcccgtc tgcgaagtac tgccccaatg gctgcaaacc tttcgcagcc tgtttctgaa
    31201 atagccgaat cacaacaacc atacatgcag cctattgagg ggaacccttt tgaagaaggg
    31261 ttcccctcaa actcccctcc gaaaacttca ctttattttt tctcataggg gctatagccc
    31321 ctatgagaaa aaatggaata aaagtctttg aaaaggggtc tggggagaaa ctttcctcag
    31381 aaagtttctc cccagtaaga ttgcatctat gactgcggtg atttggttat atcagttctg
    31441 ctaaaatggt gttggatacg aggaagccct tgggcgtgaa tgcgatgcgt tcgccgactt
    31501 tttgcagaaa gcccagcttg caataacgtt ctgccgccgc ttgcttttct gccagccacg
    31561 aagccggaac accctctgtc aaccgcatgc ccagcagcag cttttcctct ggtgtgcagg
    31621 gcgtttcgtc ttcggtttcg actggctgca ccggctgctc cagaaaatcc gacacggcag
    31681 acggcacaaa aaaccgcttg ccgttccagc aggaatgtgc agacggtccg atgcccagat
    31741 agggttcgca gtgccagtac ttgcagttgt gccggctctc gaaaccggct ttggcaaagt
    31801 tggagatctc gtattgcaaa aagccagctg cccccagctg ctccaccgcc tgcaagtaga
    31861 ggtctgccgc ggtgtcatcg tcgggacagc attccgccac gtgctgcacg gcaaaaggcg
    31921 ttccctgctc tacctttaat aagtaggcag acacgtgtgt aatgggcagc tgcaccagcc
    31981 gggaaatggt ctgctccagt tcgtccgccg tctgctccgg cagtgccagc atcaggtcac
    32041 aggacaggtt ccggaagccc gcttctgcgg cagcgtatac cgtttccaca gcctcctgtg
    32101 ccgtgtgcaa ccgtcccagc cgtttcagct gggcatcgga cagggactgc acccccagag
    32161 aaagccggtt gatgcctgcg tggcgaagtt cccgcagctg ctccggtgtc acagtggcag
    32221 gatttgcctc cagtgtgatc tccgcatctg cggacagggc aaactgttcc gccgccgttt
    32281 ccagaatttg tgtgatctgt gcaggcagca acagcgaggg cgttccgccg ccgaaataga
    32341 ccgtgtccac gcgttccggc tgggcagcgt ttccgtaatg ccgcaagttc cggcagactg
    32401 ccgcagtgta ggcggtcagc agttccggct gtgccggcac ggaataaaag ttgcagtagg
    32461 gacacttccg gcggcagaac ggcacgtgca gataaagtcc ggcacccatc agcttaacac
    32521 gtcgttccgg atcggcttca ccagcggacc gaaaaatgca tcaaagagcc ggcacagcac
    32581 gaacgctgcc gcaacagccc ccaccatgta gacgtagtcc cagttggaat tgcccttgaa
    32641 gtagaccagc aggacaacca cgagggcagc aatatagacc actgcactga agatcatcga
    32701 tgcaacgccg ttgacgcagt gctgtccaca ttccatgcag acttttcctt tggtacgcag
    32761 tccgccggca aacgcctttt tccacgggtt aaacgatttt gctccgcaat aggggcaggt
    32821 atagatcgat ttcataaatc agattccttt caaaaagttt ccggcgaggc tgaagctgcc
    32881 ggataaattt attggataaa tttattatag tgtgttccgg acaaaatgtc aaggcgacaa
    32941 aaaaccgact ttcacaaaga aagtcggttt ttcgttcagc aaggaaaaca gcatttactt
    33001 cgcgaggact tcttcccaag aagtgtcata tcctgctccg cggagagcca cgtagtacat
    33061 gatatagaag ctgtcggtgg aatccactgt accattgccg tctacatcgg cggcagcgat
    33121 ctgttcagcc gtcagcccgc cgtcattgcc tacggcaacg ttggcgatgt agagcatggt
    33181 gtaaaagatg tcggtggagt ccagcgtgtc gttgccgtcc aggtcgccct tggaaacagt
    33241 ggggtcagtg ccgatcaggg caaatttgat gttgttttca gttgcgtatt gttgtgcgca
    33301 agaatcggcg ttgccgtaga tggtcaggtt gtcacaaccg gaaaaagcat cttcttcaat
    33361 gctggtcacg ctgtcgggta ttgtgatttt ggtcagactg ctacagcaat taaaagcata
    33421 atatccaatg cttgtcacac tgtccggtat tgtgatttcg gtcagactgt tacaattaag
    33481 aaaaacttga tttccaatgc tcgtcacact gtccggtatt gtgagtttag tcagactact
    33541 acaaccaaaa aaagcattat ctccaatact tgtcacgctg tcaggtatgg taatttccgt
    33601 tagactggtg caatcagcaa aagaatattc tccgatgctt gtcacgttac ctggcattat
    33661 gatctttgct aaatcactac acgatctgaa agcataatct tcaatactta tcacgctatt
    33721 cggtattgtg atttcagtca agctgctgca attgaaaaaa gcatcttctc caatacttgt
    33781 cacaccatca ggaatgatta cagctccagt acaagcttta ccgttaatca aaatatggtt
    33841 aacgattacc aatgggtttt ctgcctgctt tgcttcaagc caaggcgtac catcaaaagc
    33901 taatgttcca atgtgcacca cgctgtccgg tatcgtgatt tcagccaggc tgctacaacc
    33961 gctaaaagct aattcttcaa tgctcgttac gctgtctggt attgtgattt ttcttaaact
    34021 agtgcaccca ctaaaagcaa aatcccaaat tctcgtcaca ctatccggta ttgtgatttc
    34081 agtcaagctg gtacattcag aaaaactctc cccaccaata ctcgttacgc cgtttggtat
    34141 catgattgta gtcagactgc tacagccacc aaaagcaccc cctccaatac tcgtcaccgg
    34201 tttcccgtca atttccgccg gaatttctag ctctccagct gcgtcgttat cacaatttgt
    34261 gattccaact gtatcatcgt tatacacttc atattccaat tgtgtcgttc cagttcccac
    34321 acgaacggta gtcgttcccg ttcccacagg gactctttct gtttcagccg tttcctctgc
    34381 actcgctgac agcactgtcc ctacgctttt cagcacgttt tccaccccgg aaagccccac
    34441 gctccccagg caaagcatcc cggcggtcat agctgccacg acttttttcc atattttcat
    34501 tttgaacacc ctcctaaatt tgttatagta aaaggtttcc atttccatta tatcatatct
    34561 gcgaagaaaa tgcaagaaaa aaatcaaaaa gcaccctaca gaaccgtttt tccgttctgt
    34621 agagtgccta ttcacatggc ttattttgcg agaacttctt cccaagaggt gttgtgtcct
    34681 gccccgtgga gagccacgta gtacatgata tagaagctgt cggtggaatc cactgtgccg
    34741 ttgccgtcta catcggcggc agcgatctgt tcagccgtca gcccgccgtc attgc
//
LOCUS       DAWBGR010000005        31048 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_572_length_31048_cov_4.149227, whole genome shotgun sequence.
ACCESSION   DAWBGR010000005 DAWBGR010000000
VERSION     DAWBGR010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 31048)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 31048)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..31048
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_572_length_31048_cov_4.149227"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2561..2570
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11728..11737
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7625 a         7512 c         7076 g         8815 t
ORIGIN      
        1 atgatatact ttaccttatt ggtttgtaaa tgtccaataa aatgaacttt tgcatctttt
       61 gcataaaaat cttttttaga aagatattcc tgcacgcggt tttcacccaa ggtgtggata
      121 ccacagctga tcgcatgatt taccgcttcc ggggcaaccg ttttggtgac cgccatcaat
      181 gtaatttcgt ctgtcggctt acggtacttt gcctttgctt cctccatcgc atcgacgatt
      241 cgtttgtagt tttcgtcgat ataagcaaaa ttagtttcca tttgcatcaa tcccttctac
      301 aattatggaa tcataaagca ttaaataacc atcgcctgca ttcagtttag aaagcacata
      361 gtctttgcct tcgtaaacta catccagacg tcggaattcc actttttcac cgtcctgcac
      421 ataaacgccc cggctgttca cggtctgtgt tttttcttcg ccggtttctt catcagtgac
      481 ggtttcttct acgtctttga aacggatcgc actccgcggc accttgatgc cctcatattc
      541 tccaagaatc atttcgacat tttcagttcg attctgtaca aagtccgatg ttagcatatc
      601 gctgtataaa accaccatga ccttgccgtc ttctccggta tttcggagtt cctgaatggt
      661 ggcatctgct gttgcggaag acgtcatcag acgaatcttg acagaatcac cgacctgata
      721 tttctgatcc tgattatcaa taattccaag catgtaccag ccgtactgtg caatggattt
      781 tcccactgtg ttttgatctt gattcgtttg gagattattt tcgctacttt ttttagtaat
      841 atcctgaatc tgttctggcg tgatatttgc cagattttcc gcagtcagta cagactccag
      901 cccatccaca tggctgacaa aataggcaga actatctgat gtaatagtgt ctatgggtgt
      961 tgcttcctcg ctttgcaaag tctgtatctg tgcagtcaaa tcagaaattt gctgtgcaaa
     1021 agaaacggtt cccttgctga caacgatctg atatgtgctg caggattcca taaactgttg
     1081 ttctgcggct gccatgccgg agagatttcc agcgtttcga ttctgaatca tctcttcgta
     1141 gtactgagaa atctggcggg ataaatccgc aggctgtgct tgttccagcg ttccgggatt
     1201 ggagatacgt tccaaaacat tcagcttttc ctgtaatgcc tctgcctgtt gttttttttc
     1261 aatggtgctc tcgtccggat aaatttcggc aatcacatca ttgactgcaa tttttcctcc
     1321 gtccggtact tgataactga ttgcaccagt gtcgctgtaa gtcagtactg tttcgtcccg
     1381 tacatagacg ccctcgaaga atttggattc atctgctttg gcacgcatgg ctgtttctgt
     1441 ttcatagcta gtgtccacaa ggtgaaagat gatcgtgcca aatgtagcaa tgacagaagc
     1501 cgacagaagc actaacagca cattattcat tttattgctg gaattcatat gcagatcacc
     1561 tcacaaaacc aacggatttc cagatgcttt tgcatcattc tacagaatca ctttctaaaa
     1621 tggaaacagg acgtatggga ataatggtgg aaatggcgtg tttgtatacc aagttttgtt
     1681 ttccgtcaca ttccagaatg acaatatagc tgtcaaaccc ttttacaatg cccttaaatt
     1741 gaaaaccgtt tgtcagaaaa attgtaacgc cgatgtgatc ctttcgggct tgattcagga
     1801 atacgtcctg aaggttcatg tttttgttgt tcattgttag actccttttc tttgagcgaa
     1861 aaataccgaa tgcgctttgc attcattttc tctttgtata taaatgctca atttatatta
     1921 tagcacattt ctccggactt ggctataatt ttttgacact tttccataat ttctggtttc
     1981 ttgtcagatt tgtctaaaat aatccactga attcgatcat ttttgcggaa ccatgtcaac
     2041 tgtcttttgg catatctgcg ggtttcctgt ttaattttgt cgatacacgc ctgcaatggc
     2101 atcttttttt caaaatatgg aatcagttcc ttatagccaa ttgcgtgata agcagtccga
     2161 agtgcttcgt tttgatacac atgataagct tcttcgatca tgccttgttc tatcatttga
     2221 tccacccgtg tattgattcg ttcgtaaagc accgaccgtt cataagtcag gccgatgatc
     2281 agggattgat aaggacttgg gatctggtga ctttcggctt tgcatgcggt gaatgttttt
     2341 cctgtcagcg tacaaacctc cagtgcacgt atcacacgca ccagattatt agggtggatt
     2401 tccgctgcgg cttctggatc caatgctttt agctggtcat gcagtgcaat gttgccgtgc
     2461 tctttggcgt attccagaaa tttttgccgt agtgcagcgt cctgcgtagt tggagccagc
     2521 tgcacgttgg aaagcaatga tgaaacgtaa aggccagttc nnnnnnnnnn ggcaggtgct
     2581 ttcggtgata gatttctgca attgtcggtt ttgctaacgc cagatagtct gcaacgctga
     2641 atgtggcatt cacagggaca cagccgatca gatgatgcgg cacaccctct tgctcttccg
     2701 gtgtgggctt tgccgttgca atattcagtc cctgatagat ctgcatggaa tctgcggaaa
     2761 caacttctcc atgatatgct tttgccagca tcacgccaag tgaagttttt ccggatgccg
     2821 tggggccaac cactgcaagt actggtattt tttctgaatc tgtcatgacc tataccctcc
     2881 gccggaattg tttttccaga tcgtattttc gaatcacaaa cattaccggt ctgccgtgcg
     2941 gacagtgtcg gacattttca tcgtcccata cttgctgtgc cagcttctgc aattcttgta
     3001 tggtggaatg atcgccggaa cgaatggcag atttgcaggc aatggtgtgc agagtatcgt
     3061 tgaaagtgtg tacctgcgga tcgcgttttc ctagtactag gttatgtgcg atctctgtga
     3121 caacctcatc aatattcagt ggcagtacga aagacggcac ggcagttgcc cgcaattgcg
     3181 gcggactact gcaatcaaag gcaaatccca tttctgctaa ctttggaaga ttttcctgta
     3241 ccgctgaaaa ctcttccatg gaaagcaaag tttcgctggg gcgaagcaac atctggctgg
     3301 tcagctttga tgctccggag cgaagctgtt caaagagcac tcgctcatgt gcggcatgtt
     3361 tatcaaaaat cagcagtttg tcttcttctg cagcttctgc aatgatataa ttctcaaaaa
     3421 tttctcccag cacgtgaatc tgtacatgct cttgagcctt gggttctggc actgctggga
     3481 gtgtttcttt tgctgctgat acaggatttt ctgcaaagga acgactgttg agaaactcat
     3541 actttccttc agacaactct ggaatggaaa ccggctccac aggtcgagat ctttcggttt
     3601 gttctgtata tgctgtttct tccagttctt catcatgaaa ttttgtttcc ggaagaacga
     3661 ttggcggaac tgcaatggtt gccgaagcgg tgctgaccag tggggcaaca gtttcccttg
     3721 tcgtttcttt cggtgccggt tctgctgctg gcttttctgt cggcggaagg agcacttctg
     3781 gttctggctc tgagatctgc ggcaatgctg gttgcacaaa ttcctgttga ggttctggct
     3841 gtgcagtcca atcccgctgt ggaacatgat ccatttgaaa ctcataaatc aagccgtttt
     3901 tcatcagggc atttttcact gcgaagaata aactgtccat gactgctttt tcattggaaa
     3961 aacgaacttc tgcttttgca gggtgaatgt ttacatctac ctgttccggc ggaacttgca
     4021 gcatcaacac gcaggtggga aactttccga ccatgatcaa attctgatag gcgttttcca
     4081 aagctacgct gcactgtctg gatcgaacat atctgccatt gataaagaaa ttctgaaacg
     4141 ttcggttgga acgggagtac aaaggctttc ccacaaaacc agtcacctga ttcatgccgt
     4201 cctcataaga aaccggtatc aagtcatgag cgaactcttt tcccagaatg gcataaattg
     4261 cagtgtacaa gtcaccgctg ccgtcggtac ggaattccgt tcggttatcc cgtatcatgc
     4321 gaaatgcgat ttctgggtgt gacaatgcga ttttctgaac gatctggctg acggcgtttg
     4381 cttcggtcac atctcgtttc atgaattttt tccgaaccgg aacattgtaa aacaggtcgc
     4441 ggatcaaaag tgttgtcccg tccggacaac cggtctgttc caattctcct gctgtactgc
     4501 cggaaatgct gtagctggtg ccgaaatcat cctcttttcg cttcgtcaat actgtgactt
     4561 ttgccactgc tgcaatggaa gccagagcct caccgcgaaa gcctaatgtg aaaatgtgat
     4621 ccagatcggt tttttctgca attttactgg tggcgtgacg gagaaaagca gtaggtactt
     4681 cctccggtgc catgccgcag ccgtcatcta cgattcgcat gtaagtggta ccgccatgct
     4741 gaatttccac tgtgatgtgt tttgctccgg catcgattgc attttccacc acttctttga
     4801 taacagaaga cggacgctca atgacttctc cggcggcaat caattcagaa atttctttcc
     4861 gcaaaactgt aatatgcggc atgacaaaca ccttctttct aagcggtatt ttaccgcgga
     4921 catgtgaaca cttttattaa agcgtgttta catactagga cagcatcgat gtgagatcag
     4981 ccagtagttc cctgcattct gcatcactta attctgccag atgtgttttt tgcaaggtgg
     5041 caattgcccg ttcctgctgc attgctgcaa aactgatctg tccagtctgt gcggtttgct
     5101 tcggggcgga tactgctgtc tgtgcttcca tttctgccag cagttccttg gcacgtttga
     5161 cgacctgatc cggcagccct gcgagttttg caacagcaat gccgtagctg tcatcaacgc
     5221 cgccaggaac gatttttcgc agaaaccgaa tggtatcgcc gtgcttcttg acagcaacgc
     5281 tgtaattttt taccccgtcg atttcttgtt ctaaatccag tagttcatgg taatgggtgg
     5341 cgaacaaagt tttacagccg attttccggc tgctgatgta ctctgctaca gcacgtgcga
     5401 tgctaacacc gtccaacgtg gaagtgcctc tgcctacttc gtccagaatt accagactgt
     5461 tcttggtggc ataattcaaa atgtctgaaa cctctttcat ttccaccata aaggtactct
     5521 gtccggctgt cagatcatct gatgctccga tacgtgtgaa gatcttatcc acgacagaaa
     5581 ttttggcata ctctgccggc acgaaagaac cgatttgagc cattagcgta atcaacgcca
     5641 cctgacgcat gtatgtagat ttacctgcca tatttggtcc ggtgatcatc agcatacgct
     5701 gtgctttggt atccagatag gtgtcattcg gcacaaacat ttcctctgtc agcatgcgtt
     5761 ccacaacagg gtgtctgccg ccggtaatct gaatgctgcc gtctactgcg atttccggtt
     5821 ttgtgtagtc gttctcaatt gccacattgg caaatgaagc cagcacgtca acttgtgcaa
     5881 ctgccgctgc tgtttcctga acttttttca gcatggcagc caggcaatcc cgcacttcgg
     5941 agaaaatgcc ttcttccaga cgcagtacgc ggtctttggc tccgagaatt tcattctcta
     6001 catttttcag ttcttctgtg atgaaacgtt cgctgtttgc cagtgtttgc tttcggatat
     6061 agtccgaggg aatcaaatca taataagaac gagtaacttc cagataatag ccaaagacac
     6121 ggttatagcc aattttcaaa cccttgatgc ctgtacgttc tttttcccgc tgaagaattt
     6181 catcgataat ctgtgtgccg ccgttcataa tgtggcgttg ccgatccagt tcttcatgga
     6241 agccgtcccg aatcacgccg ccgtctttca aagtgattgg cggatcatct acgattgcat
     6301 tttctactaa tgtacacact cgttccagcg gcgaaatttg ttgctccagc gttgccagca
     6361 agcgggaatc ccctgccagc tgtcgtagct gtgctttcag ttccggcagc tgctgggcag
     6421 tcatggagag tgacttcaaa tcccgtggag tggctttctg gtaaatgact cttgtcatca
     6481 gacgttccag atcatagacg ttgttcagca gatctcggac ttccattagc acaaggcttt
     6541 tcttgacaaa tgcatctact gcgtccagtc ttgccatgat cttgacgggc tgcaccagcg
     6601 gctgttccaa ccatgttttc aacagccgtt tgcccatagc ggttctggca tcgtccaaca
     6661 gccataacag cgaacctttt cgctccttgt tgcgaatggt ttctgtcagc tccagattgc
     6721 ggcgggcgtt caagtccagc cccattgctg aagcactgtc tgcaacttca atggaaacaa
     6781 aacgtgcgat gtttcgcttc tgagtttccc gaatataatc cagcatacca cagacagcac
     6841 tgacatcagc accgtcttct gtcatgccca acaaatccaa ggaaggaaca ccgaattgct
     6901 ggcataccaa ttcccgattt tgtaccggat cgaagcagat gtcatctcgc agagaaactg
     6961 cacactgcag ccgaactttg ataaaatcca taacggactt caaagagagt gcctgactgt
     7021 tcattaccag ttctgccggc atgtagcggg acagcacgtc cataatgcgg acagaaaggt
     7081 cagatgcttc ctgtggtact gtcagggaca tttctccggt ggaaaggtct gcgaagcaaa
     7141 gtgcacagga accgtcattt ccgtaataca gggtacagat atagttattg gaatcatctt
     7201 ccagcatgct gctctcgatc agcgtacccg gcgtaacaat acgaatgaca tcacgttcta
     7261 ccaagccttt gctcaatgct ggatctgtca tctgctcgca aattgcgatc ttgtatccct
     7321 tatccaccag acgtttcaaa tagagttcta cactgtgata cggtacgccg cacatgggag
     7381 cccgttcttc cagaccgcac tcttttccgg tcagtgtcag ttccaactct ctggaaacca
     7441 gaatagcatc ttcgaaaaac atttcataaa agtcgcccaa tcggaaaaac acgatacagt
     7501 cactgtattt ttctttggtc gctgcatact gctgcatcat aggagaaagt ttttctctgg
     7561 ggacatctgc taagttcatg ggattcacct ccgataatca cttcagaact tgtttcgcga
     7621 caggtgttca gtgacagccg ccgcaggaag aacagttgcc agtgcagttg gtcagatccg
     7681 ggtgacaccg atccggatct tctccgtttg cacacaggga caaaatggtg tccacttcct
     7741 gcatcatcat attcattgcc tgcttggcag tatcaaaagc ctgcatgttc gggttttcca
     7801 tgatctgcaa ataaagtgcc tgtatctcct gttccagttt ttccaatttt tccggattcc
     7861 ggttttcctc cagcttttta gtttcatgct gataggacat ttttttcaga ttaaatgcct
     7921 gtatattttc ctgcagttct gtatctgcat cattatcggt tttggctttc caatacgcct
     7981 gataacgcgg gtctgcctga attgctttgc ccaattgtct taccgcagct tctacttctg
     8041 tcatgcctgt tcctcctcta ccagttcgcc aagtactgcc cagtttctgg cttctgtaat
     8101 tctgacattc tgaaatgtgc cgatcaggga ggggtcaccg gaaaattcca caataatgaa
     8161 tgcactgtcc tttccgacca gtctgccttt ccgcttgctc tccccgtcta ccagtactcg
     8221 cagtgttctg ccaatgaagc gtttgttgtg ttcagaagcg atgtcccgct gcaaggcaag
     8281 caaacgctcc atacgatttt tcttttcagc atctgtggtg tgatcttcca tttccgcagc
     8341 cttggtgccg cggcgtttgg aataaataaa tgtatagatg ttatcatact gcacacttcg
     8401 gatcaaatcc agtgtgcctt gaaagtctgc ttcggattca tccggaaatc caacgatcaa
     8461 atcggttgtc aaggaaaaat ccggatctct ttgccgaaga tagttgacac tctccatata
     8521 tttttctacg gtgtagcggc gattcatgcg gcggagcaca tcatcgctgc cgctttgtac
     8581 cggcaaatgc aaatggtgcc cgatcttcgg acattccaga attgtgtcca gcagttccgg
     8641 cgtagcatct ttcgggtgtg acgacataaa ccgaactaca aattctcccg gaattgcatt
     8701 gacttttcga agtagctgtg caaaggtcag cggctgctcc aaatcttttc cgtaggagtt
     8761 tacattctgt cccaacagca tgatttcttt atagccgtct gctaccagtt gtttgacttc
     8821 atttaaaatc tgttccggat cacggctgcg ttcccgtccg cgaacatacg gtacaataca
     8881 gtatgtgcaa aaattattgc agccgaacat aatggggaca gaagctttga acgggtgctc
     8941 ccgtgacggt acgattccct catacatggc gtcgtattcg ccggtatcac aggcaaactt
     9001 ttttccctga aaatgttcca gaagcaactg tggaaaacgg ctgagagaag cagtaccgaa
     9061 aacaatatcc acaaagggat aatgtgtccg gacaaattct gctgtatgtt cttccgatgc
     9121 catacagccg caaagcacca gaattagatt cgggtttgcc tttttgtact tttttacatt
     9181 gccgagaatg ccgtataccc gatcttccgc actctcccgc acggcacaag tattgaacag
     9241 aatcagatct gcatcttcat aggaatcggt cattgtatag cccagttcga tcagcatgcc
     9301 gcgaattcgt tcgccgtctg aaacattcag ctgacagcca aaactatgca catgtgcacg
     9361 cacaacgtcc cgatcttgaa atagataggg ttgtaactga tgccgcatgg cttctgtcac
     9421 tgctgtcatt gtctgattgt tccgacattc cataattctc tatctccgtt tctgtattcc
     9481 attgaaaaag gtcattacac tttattatag cataactcac ttttctttgc aagaattttt
     9541 ttcgaatttt ctgcaaaaag caaggctttt tctgaatttg cccttcggaa ttccattccg
     9601 aaatttcagg aaaatcgcag aaggggattt tcagataatt tccagccact tttcacaagg
     9661 caatcacaaa ccactgatat actgttacca tcgtcatttg aagcaattgt ttcacatgtg
     9721 cataagtacg gtgcaagagt gccgctgata ttcttaccgt ttcttgcaag aagcggacca
     9781 accatactcg tacaggaatc tatcactcct ttccaccgtt tcaaacgggt aacctctttt
     9841 tcatcatctt cattttcaaa gtttggtttt gcagcggatg cacgcataca cctgcaaaac
     9901 aaatttttgt tcctgtacgt tcctataaat tgaactgaaa ccggcaggtc tcctctcctt
     9961 ttccctgccg gtttcttctg tttggaggta tattttatgg cagaacgata cttttctgca
    10021 tcggcgtatt atcagcagac atttggcaga ccagtacaga aagcagtatt ggacattggc
    10081 tgcacttgcc ctaatattga cggaactatc ggaaccggcg gctgtatctt ttgtgacggc
    10141 ggcagcgggc attttaccca tagcggcacc gttacacagc aacttgcaca agaacgacag
    10201 cgaatccagc agcatatttc tgaagcaggc atcattgctt attttcaggc acacaccaat
    10261 acatatgggg atctgcatta cttggaaagc tgttatttag aggcattgca gcagcctgat
    10321 gtagtcggaa ttgcaattgg tacccgtccg gactgtctat ctgaggagat cctttctgtt
    10381 ttggaaaata tttctgacaa aacgcatctg actgttgaat tgggattgca gactattcac
    10441 gaaaaaacgg cacgtttttt tcacagaggg tattcttttg cggtttttca aaaggcgttt
    10501 gccgcactgc gggagagaaa gattcgaatt tgtgtgcatt taatcaacgg gctgccgtta
    10561 gaaatagatg cagatatgtt ggaaacagcc cgtgggattg gcagattacg ccctgatgct
    10621 gttaaaatcc atttgctgca tattcttcgg gatacacctt tggagcaact gtggaaagct
    10681 ggaaaattac agccactttc catgcaggaa tacgtgcaga ttactgcggc acagttaagc
    10741 ctgctgccgc cggaaactgt agtggaacgg ctaactggtg acggtgcttc tgacagattg
    10801 atggctccgc tatggagcag ggataaactt gcagtgcgaa acgcgattgc tcgtcatctg
    10861 aagcagacag ataactggca gggaaaatac tatcaaacat agaaagccgg acgaatgtgc
    10921 atcttcgtcc ggctttttgc gggtacaaaa aatccggaac ggtagacctc cattccggac
    10981 atttttattc ggttcatttt atgaaagaac tttcattctt tcgcatcagt ttcgattgga
    11041 ctcactcctc acgttgtttc gtaccataca aatcgtcttt ttaaaagcca cacattaaac
    11101 acgtcctttc cgatagaaca cgtttcctat gatctttgca agtacaatat caagccatgg
    11161 gactcacctt ttctgttata tttcaaagaa aagcttttct ttccatggga atcacctttt
    11221 tcattcgaag tgactgataa atcaaaacat tttacttcat gggaatcacc tgagattgtt
    11281 acttgggaga tcagcatact atgtaaaggc aactatgtgc cggagatttc atgccgtcat
    11341 cgcacgaaat taaatatatg ctgccaattc aaataacaga tttttcatgc agacagttgc
    11401 gaatgtcatg catcaggtgc aaccagctta cagatttttt tatcatttct tacgtgccca
    11461 cagccaatcg aagttgactt ctcgctacgt tcttaatcct gcatcaccgg cgattcaccc
    11521 gatattgaca gcatatccaa ctgtgattct tcacttgcca tcggatctcg ccctcccttt
    11581 tgatgtaaaa tcgtttccgc aaaaggaatg aaccttaaac cttgggaaac ttatcgctcg
    11641 ggaatcattc aacttagatc ttttcgccag ctccttgtcg ctgttccctt tcgttgttta
    11701 tatcatacca cacttcaagt gcaatgtnnn nnnnnnnacc ttatataatt ttttaaagga
    11761 taacttattt gcataaacgc atactgtttc tatgctgctt tgatttaaaa attatattta
    11821 aaatgaaaca cacggatacg tggatagaaa tgagcgtatt tccttagata tacgcaaatt
    11881 aaaaaggcgg agcaagggaa aacttgctcc gcctttttaa tttattgaat tttcaaaacc
    11941 gaattactgt gctggtgctg cggtttgagc tggttctgta gcaggcggct gttccggtac
    12001 agtttctgca ggagcagctg tggtttcttc tacaggttct gtttccggtg gtgcagtcgt
    12061 ttccggttcc ggattatagg cataatatac gatgacctga tacttgtcgt tcagattgat
    12121 cgaatctcct gcccccagat cagtttcctg tccgtttttc gttacaacaa tacggatgac
    12181 actgccgttg ctatagccgg aactgctctc tgcagattca gtataaggaa tgttgagttc
    12241 gtccagtttt tctccgtaag agttgtgtcc gcttccggag taagacggaa tctcaactgt
    12301 tgcggagccc ttgctgactt gtacttttac ttttttagat gaatcaaaag tatcgcctac
    12361 ttcatattcc tgccagcaga tctgtccggc agcatactgg tcactgaata cttcctctgc
    12421 atcaaatgtg atccacgaag cgtattgcgt tttagcattc tcaaagttct ttcccagaag
    12481 ctgcggcatc acgctgtcac ccactgctgc cgttgtagtc acttcctgtg tttcagaaac
    12541 agcagtagtt tcagaaattg ctgttgtagt tttactggat tcggaagaat ctcctttgaa
    12601 caggtcacca gctaccacga ataccacaat cagcaaaatc aacagcaaga caattcccac
    12661 aatgatcgga acacgcagac gatctactgc tcctgccttt tgcggctggc gataattatc
    12721 ttcttcctga taatcatcat aggcgtaatc atcagaatag ccagtatcat aattcggata
    12781 gttctggtca tcataaggct gttccggata ttccgaatac ggttcatctg cataaggctg
    12841 ctggtttgca tatggttgct gtccgtagcc accattgtcg aaaccgccat aaatggcatc
    12901 ttcttcctct gcatgttcgt caaacaaacg agttaccaat tcggttaccg ttttggtacg
    12961 ttctgttccg ttcaggttca gtccctgcat gattacattg gaaacatgac gaggaatgtt
    13021 ggggttcaga actgccggtg caaccagatt gtcattttcc atacggctga ttgcagaggg
    13081 cggcatagag ccggtcagaa ctctgtaaat gaccgcacat acagcataga catctgtcca
    13141 tgtgccctgc tgtccgttgg cagagtactg ttccggtgca gcgtatccat tgtaaatttc
    13201 gcattccagt tccgtattgg cagtgcggac tgcggcaata gaaaatgctg tcagcagtaa
    13261 ttcgccctta tctgttacat aaatagtatc tggtgaaatt gcacggtgca ctacgccgga
    13321 attgtgtagc agactcagtg ttgtcagcag cggcggtagc atatgtgaaa gctgtcccca
    13381 tgtcaattcc ccggcattat ctttcaggta gtctacaaag cggacactct ggatatattc
    13441 atctaccaca taagtagtat tgttttctgc aaacaggtct accactgtat tgacatgcac
    13501 ttgtccgcga agccgtgcca gtgatttatg cagttcggtg aattctgcca tcagtgcctt
    13561 atattgtgcg agccgtgccg gaatcacgct gatcacaggt gaaccgctga cacgattgca
    13621 cagatttggt ggcatatatt cccgcagata tactttacag ccaatggatt ggtcatacgc
    13681 aagataagtg ataccttctc cgttgacaga cagtgtcaca ccgacaatat aacgattatt
    13741 gagttttgtt cccggaatga taaaagacgg gtcactcgga gcgttgtcgt aatatccaca
    13801 attggggcag actccgccag aaaattctgt gcgttccatg cagccatagc ataatttccg
    13861 ttctcccaaa gcgatacctc ctgttttttg gttcggcagg ttttattatt aaaattcctg
    13921 gatttggaaa tctgttagga attcaaattc tgcaaattat attttaccag caactctaag
    13981 cgatgtcaac tgttttccct gaaatttcat ttgatcctgt tcaaattgta tccatttgta
    14041 atagatttga tactgttttt cggtatcaga gcgaaatcaa tccttgatcc agcatctttt
    14101 gtgctgccag aagcacgccg atctttccgg aagtataggt catgccaccc tgcatccaga
    14161 ctgcaaatgg ctcccgaatc ggagcatcag cagacaattc aatggaggct cccatggtaa
    14221 acgcaccagc tgccatgatg actttactgg tatagcctgg catatcccac gcctctggta
    14281 ctacaaagga atccacggga gaaccctgct ggattccctg acaaaatgca gtcagatgtg
    14341 cttcatcccc cagacagatc gcttctacaa tgtccccacg tgttgtacct actggtgggt
    14401 ggcaggagaa gccaaagctg ccgaacagag cagctgcaaa gtctgcggtt tttaatgcat
    14461 ttgccgtggt atccggtgca tggaagaagc cgagatacaa ttcccgattc atgtccagtg
    14521 aacagcctac ttccttgccc ataccgatgc aggtcagacg ctctgcacac tgctgcacaa
    14581 gggcttcctt tccggcaata tagccgccgg tgcgggcaat accgccgcca acatttttaa
    14641 tcatggaacc gatcatcaga tcacagccaa cttctaccgg ctcgctggtt tctacgaatt
    14701 cgccgtagca gttatccacc ataatgactg cgtctggatt tgtgctgcga accagttctg
    14761 ccagccgttg gatttgtgct acggtcagtg ccggtcgtag agagtaccca cgggaacgct
    14821 gaatataggc aacttttgct ccgcgaacgg catcaggaac tgcctcataa tccggtgtac
    14881 cgtctttcaa aagcggcact tcccgatagg tcacgccgaa atttttcaga gagccactct
    14941 gggtatcgtc gttcaatccg attacgccct ctaatgtgtc gtaaggtctg ccagtcagag
    15001 aaaccatcgt atctcctgtc cggagaatac caaacagtgc cactgtcaga gcgtgcgtac
    15061 cggaaacaaa attatgccgt accagtgcat cttctgtgcc aaatgcagtt gctacgactt
    15121 tatccagaac ctctctgccc tgatcgccat agccataacc agttgtgcca tagaaacaag
    15181 gttcactgac acggcttttc tggaaagcag ccaagacttt ggcaccatta taggcagcgt
    15241 tatcctcgat tctggcaaag tcggctgcac agcttttctc tgcggcttct gctgccgcag
    15301 caagcctgtc gtcaatggga aaaaattttt gaatctgcat agcttacagt gctccttttt
    15361 cttgctgttc tttgggaata tcctgtcgtt ccaatacata ttccgattca atttcctgaa
    15421 atccgatttc ccgataaaag ctgattcgca catccaatgc aaacagagca gcctgtttgc
    15481 cctctcgtgc cagttctcca gcgatccacc gcaggaaatc ccgtgcataa ccgcttccac
    15541 gctgtgcaag ttttgtggca acttgtccca ccagcacatg tcctttccag tcgtacacca
    15601 gtgtcagcgt agaactattc ttataggtca gcacctgact catatggtga cggcagcgat
    15661 gcgaaacgtc tgtcagccag agcgaatatt ccaacaaatt gggaaatcct tcctgcaaga
    15721 tctgataaac atcttgcagc ttgggcgtgt cattaatgtc ctctggacag aataccggtg
    15781 agggctctga aggcgttagc tgaaatgtgg aacggtggag tttttgatag tttggaatct
    15841 gcgataggat cggcagcaac gatgccggac actccacccg aaacggcaga tgcatagcag
    15901 tgaatgtgca gatttcttct tccggcacat ctccttcggt gcagatcagc agggttgcat
    15961 taaacaaaaa cagccagcca gtgccgccac agcctgtcac ccgaaagaag cggcagaaat
    16021 cgtagctgcc gccgtatgct ttctgatagg caagcatttg tctgccacgc cagctttgtt
    16081 ctagaattgc ggtttccgct gctgaaaatg cagtgatttg ttgtatcata tcgatgaaac
    16141 ttcttccatt atggatttta tcagcagcgg cagcaaagcc gtgcaagcat caaggtcttt
    16201 caaatccgcc agacaagacg gcgaatgcag ataacggcaa ggcaaagata ttgccagtgt
    16261 ccgtacacca ccgcggctgg catgaattgc accgctgtca tttccgccgg caatgcggga
    16321 tttggtttgg cacggaatct tgtttttttc gcagatggaa aatgccagac ggtataattc
    16381 cttatcataa attgcaccgc gatccataaa ggatattacc ggaccttcgc ccagactgca
    16441 cacttttgca tcaccctctg caccggcaat gtctgcggca gtggtggctt ccagaaccag
    16501 agcaaattcc ggattgactg tataggctgc cgcttttgcc ccacgcagtc cgatttcttc
    16561 ctgcaccaga aaagcagcgg taaaatcgta gggggcttcc tgctccagca ggtgcagcag
    16621 catagcacaa cctgcacggt catcaattgc cttgctgcaa acacggcttt ttcccatgcg
    16681 ggtaaagttt ggagcgaaat agacataagt tccctcgcgg gctgctgtcc gccgttctgc
    16741 ttccggcaga atacccatat ccagaacaag ttcgtcccac ttgggcaatt ttttcttttc
    16801 gtccgcagac aacaggtgca ccggctttgt accgattaca ccatgcagat gctcttcacc
    16861 gaccagcact tgccgtccca gtgcaactga tgcatctacg ccgccgactg cttccagaca
    16921 gtatgtgctg tcggagttga cgtgagtgac gatcatgccg acttcatcca tatgggcact
    16981 gatcatcagc ttgtgcggag cacgtttttt tccctgtttt tgtaccagca aactgcccag
    17041 cgggtcgatt tcccacgtaa catcttttcg ttcccgcagg acagagagaa tataattgcg
    17101 aacttctgat tcgtcaccgg acgatgcaca gagattacac agggtttcaa aatggttcag
    17161 catgttccgc acctcctaag gtaagcagcc agcagttcac cgtttttttt cacatcagag
    17221 atctgaatta cttctgccgg tgtgtgcata taccgcagcg gtatggatac cgttccggca
    17281 caaacgccgc cctttgtgac agaaaagcag tctgcattgg taccagttct gccggacatc
    17341 acttccggtt gccatgcgat tttttctgca tctgctgctg caattaaatc atcagtaact
    17401 ttacgggaaa gagaaggaga aatgccgatc atcggacctt ttcccatgct gccgcatttt
    17461 gtggcatcac cgttgtgttc cagtgcaaat gtcacatcta cagcgagggc aatatccggc
    17521 tcaatgtcat aggcaccgat cgtggcaccg cgttcgctgg tttcttcctg tgtggaaaac
    17581 agaatggagc agctgcatgg cagttcttct ccctgtatca gaaacagagc atagagaata
    17641 gaagccatgc cgcagcggtc gtccagagaa cggctgcata caaaatcgga ttgcagcggt
    17701 acaagcggca tatcgaaaga aacagtatct cctgcttcta ccagttgctc cagttcttct
    17761 ttggtatatc cggtatcaat tgcaagttct gtgacagaga gcacatgttc ttcgccgttc
    17821 tgcaagtgcg gcggcataca gcaaatcaca ccggcaatgt cctgttttcc atgaattacg
    17881 acacgctgtg ctggcatgaa ccgacagtcc aacccgccgg catttccaac acggacaaaa
    17941 ccgtcctctg taatcgcagt gaccagaaag cccacctgat ccatatgggc atctaacagc
    18001 acatgtggct tgccgctttg gcgttcgccc agtatgccga tgacatttcc ctgtcgctgt
    18061 tcaacagtgt cacagtacgg tgttagcagt tcctgagcca ctgcagcaat gctgttttca
    18121 ctgccggaca cgccggaagc tgctgtcagc tgttccagca aattcaacaa atccatgtca
    18181 gtgcagctcc tttaccagat ttttatagtg gattcctctt gcttcaaagt tcggatagag
    18241 gtcgaagctt gcacaagccg gcgacagtgt aacaatatcc cccgtcttgg cacgctttct
    18301 tgcctcctgt actgcttctt ccatggagcc aacatgtacc aactccggag caccttcctg
    18361 atagaactgg ctgccgcgga tcgctgcttc gattttatct gcggttacgc ccatgagcaa
    18421 taccagcttc acctgtttgc agacggtatc tgccattggt tcaaaaggaa tcttcttatc
    18481 atatccgccc ataatgacaa tcagtttctg ttggaaagaa cgcagtccag ccagtacacg
    18541 tgtcggactg gtggcaatgc tgtcattgta ccacttgaca ccgtccagtt cgcggacgaa
    18601 ttcgatacga tgttctacac cgccgaaatt ctttgccaca tactgtatat cttcgacaga
    18661 aactgtgccc catacggcat tgattgcagc caggtagttt tctacattgt gcatgcccgg
    18721 aatccgaatg tcattcatgt ggacaaccgg tgtgtccttg ccgtctttgg taaagcacag
    18781 cataccatct tcccgcagat aggtgccgaa ttccggtttg gttctgcggg aaaacgtagt
    18841 cagcgtgccg cgaaccattg atgccagatg catagtgggt tcgttgtcca gattcagaat
    18901 ggttctagag aatgcatcct gatgtgcaat cagattggtt ttggcataga tatattcttc
    18961 catcgtaccg tgtacgtcca gatgattcgg agcgatattg gtaatgactg caacatccgg
    19021 cgactgccgc atggacagca gctgaaagct ggaaagttct acgacagcca catcatcttc
    19081 gcggatttct tcaataatcg gcagcagagc acgaccaata ttgcctccct tgtgtacacg
    19141 aaagccagca tgggacagga actctgagat cagtgtggtg gttgtagtct tgccgtctga
    19201 accggtcacg gcatagatct tgcacggaca gagagagaag aatacttcca tttcggaggt
    19261 gataacaacg ccctttttgc gtgcttcctg caatttttcg ttgtgatagt acatgcctgg
    19321 tgaacggaaa actacatcat aatcagtcag ggaatccaga tagctgtccc ccagacagaa
    19381 tttgactccg agagattcaa attctgtgta gacttttccc agctgttccg gattcttgcg
    19441 gtccagcaga gtaacatcaa tgcccttttt gcgaaacagg cggataatat ctccatttgt
    19501 aacaccggtt ccgataaatg ccacgcggct gttctgcatg gtttcaaaaa aacgtgcaac
    19561 gttatgcata attgctttcc tcctagcttg tgacagaaca gcaggcacga tcaaatggat
    19621 acgtgcctgc tgcaaatcat tcttttgaac aataaaaatg acttattcgt tcagaaaatc
    19681 attcatggtg atttctacgc ggggattgcc gtcagaatcg tcgtcaatca tttttacctt
    19741 ttccataaca atgtcctgat tcggtgagga ctgtgcaccg tctgaaccca gagaacgctc
    19801 taccttcagg aaagaatcta ctacgtccat accttcggta acttttccga atgcagcata
    19861 ttgtccgtcc agaaatgtgt catcatcaga atagcagatg aagaactggc tggaagcact
    19921 gtccggatcg ttggatcttg ccatggaaac tacaccacgg gtatgagaaa gattgttttc
    19981 tacgccgttt tgagagaatt cgcccttgat cgtttcagag ctgccgccgg taccgtcacc
    20041 gctcggatcg ccgccctgtg ccataaaacc atctactaca cggtggaact tcagaccgtc
    20101 ataaaagccg tccttgacca gcttttcaaa gttttcacaa gtgatcggtg cttcgtccgg
    20161 atacagtgtg atgacaaaag aactgccgtc tgctgtagaa gaatccgact tggacgaacc
    20221 gcaggctccg agggttccca tcatcataac agcactgatg cccagtgcca gaaattttaa
    20281 actacgtttt ttcatattgc attgcctttc tgtgagaact cggcaatttg ccgattctgc
    20341 tgattttttc ggcattccct tttcggagtg ccagcagagt ttgccttgta tctttcagag
    20401 cgaactggaa ggtaaaataa actcctacgg ataaatgata ttatagcatg ttttctttgg
    20461 aattgcaaga cttttttgtt atggaaacac tgcataaagc ttgtgcgtaa gatgtgtagt
    20521 cagatttttc ggcggattgt acagaaattc tgcaagcaga ttgcgtgcaa agccgtcagg
    20581 cgggctttat gcagtgcttc cttatgataa cgtctttgca atgacatcta aaattgcgtg
    20641 tcccacagct tccttggatt gcagcggcaa ttcttgttct ttttgagcct gtatcagtgt
    20701 tactacgttg gtatcggttc caaagccggc accagcaact tttagattgt tggcacagat
    20761 catatccaag tgtttttgct tcagtttggc tctggaattt tccagcatgt gctgggtttc
    20821 catagagaag ccgcagagaa actgtcctgc tggcttatgt tcgcccagat atgccagaat
    20881 gtccttggta cgggaaagcg gtaagactgc atctcctgcc tgttttttct gtttttccgc
    20941 agaaactgtt gccggacggt agtctgccgc cataataatg gcattctgtt ccgcagcccg
    21001 tttggtcact tcctgataca tttcctctgc agaggtcaca gcaacagttt gtacaaaagg
    21061 cggcggtgtg attgccgtag gaccagttac cagtgtcaca tctgctccgc ggagcatggc
    21121 acatcgtgcc agtgcatatc ccattttacc ggtggaatgg tttgtcaggt aacgcaccgg
    21181 atctacagct tcttgtgttg gccccgcagt gaccagtatc tttttgccct gcaagtcttt
    21241 tttgcaggcg atttcccgca gaatccaatc cagaagcatt tccggttccg gcatttttcc
    21301 cgctcccacg tcatggcacg ccagcattcc cacagccggt gcaatgattt tgtaaccgaa
    21361 tcgttccagc ttttgcaggt tgtcctgcac aatgggattc tcgtacatgt gagtattcat
    21421 ggcaggcgaa atcaacaccg gtgcggtgct tgccataaca gttgtggtca acatgtcatc
    21481 agcgattccg ttggcgattt ttccgatcac gttggcggaa gccggtgcaa tcagcatgac
    21541 atctgctttt tttgccaaac tgacatgtgc cacatcaaac tgaaagtttc ggtcaaaggt
    21601 atcgatcaga catttgtttc cggtcagtgt ttcaaaagtg atcggcgtaa taaaatttgt
    21661 tgcgttctgt gtcatcagca catgcacatc agcatgcagc tttaccagtt tgctggcaag
    21721 agaagcgatc ttataagccg caatgcttcc ggttacgccg agaacgactg tttttccctg
    21781 cagcatgttc tcaccgcctt atgccagttc gccgtgtttt tcatagtggg agatttttgc
    21841 cacggcgttg tgctcgtcca ccagaacaac tttcggctgc atggttttgg cttcttccgg
    21901 tgttacctga gcataggaca tcaaaatcac cttatcgccg gtctgtgcct gccgtgcagc
    21961 agcaccgttc aggcagatca caccgctgcc ccgttcaccg gcaatggtat atgtcaaaaa
    22021 gcggttgcca tttgcgatgt tgaccacctg taccgcttca tattccagaa tgccggcagc
    22081 gtccagtaaa tctgcatcca cagtgatgct gcccacataa ttcagttctg cctgtgttac
    22141 agtagcacgg tgaatcttgc ctttcagcat ggtgatattc attcagcggt acctcctgcc
    22201 tgcatcatga agttgtcgat cagacgtgtc tttccgatat aaaccgcaat tgctgccaga
    22261 aacggtctgt ccagcttcgg aatctgctgc atagtcatca ggtcaacaac ttttacataa
    22321 tcgatttttg ccagcggctc ggcttcgatg atcttggtca ttgcctgtac cagctttgct
    22381 gcatcagttt ctccggcttc tgccagctgc tttccggcaa aaatgctctt gcttaacacc
    22441 agtgcggctt tgcgttccgc aggactaagg taagtgtttc tggaactctt tgccaggcca
    22501 tcactttccc gcacaatagg gcagccgacg atttctacgt tcatattcaa atccagcacc
    22561 atacggcgga taaccgccaa ctgctgggca tctttttcgc cgaaataggc tttgtccggc
    22621 tggacgatat gcagcagctt ggacacaacg gtgcagacac cgcggaagtg taccggacgg
    22681 ctcaagccac agagctcttt ggtgagaacg ttcatatcca caaagctgca aaatcccttg
    22741 ggatacattt cttccggtgt agggtggaag atcagtgcgg caccagtact ctcgcagagt
    22801 gcagcatcgt gttccagatc ccgcggatag ctttccagat cttcgttggg tccgaactgg
    22861 gtcggattga caaagatgct gaccacaaca cggtcattct gtgcacaggc tttcgcaatc
    22921 aggctctgat gcccttcgtg caggtaaccc atggtaggaa ccaatccgac ggtcagtccc
    22981 tcttttcgcc aagccttgac ttgttcccgt acttctgcaa ctgtttctac gattttcatg
    23041 cttgtgcctc ctgttccagc tgtgccatta cttcgttaga aatggcaaac gaatgttctt
    23101 ctgtgggaaa attcccctct tgtatttcac tgcggtacga cgcgaatgct tcctgcatca
    23161 cttttccgac ttcggcaaat ttccggacaa acttcggacg gtagtccgag aacatgccca
    23221 gcatgtcctg atataccagt acctgtccgt cgcagcctgc tccggcacca atgccaattg
    23281 tgggaatatg cagtgtcttg gtgaccaatg cagccagctt tgccggcaca cactccatga
    23341 ctaatgcaaa ggctccggct tcctctaccg ctttggcatc ttccagcagc tgccttgctg
    23401 catcagcagt tttgccctgt actttgaatc cgcccagcgt gttgacagac tgtggcgtca
    23461 tccccagatg tgctactacc ggaatggaag cgttgacaat ggcacgaatc tgttcacata
    23521 ccagcttgcc gccctccagc ttcacagcac cggctctgcc ctctttcatc agcctgccag
    23581 cgttgacaac tgcgtcataa acagaggtct gataggacaa aaacggcatg tctgctacaa
    23641 ccatagcatt ctgtacacct ctggaaaccg cagcggtatg gtggatcatg tcttccatag
    23701 tcacagaaat ggtgtcggaa tagccgagga ttacattccc cagcgagtcg ccgactaaca
    23761 gcatatccat gccggcttcg tccatcagct tcgcggtggt gtagtcataa caggtgagca
    23821 tcgcaatttt ttctccccgt tctttcatcg cctgaagcgt ctgtaccgtt tgtttcataa
    23881 atcaattcct ccaaacagag aatagtacaa tttcccatgg ggaatactgt ccagtatcta
    23941 tcatagcaga aagatgtgaa aatgtcaagg cggaaagaac gattttccga ttctggattc
    24001 ctgaaaaagt gacagctttt ctggtgaaaa ccctactcat ttttgagaaa tatgctgaaa
    24061 attcgcaaag cattgacaac gcaattttgt tctcgtataa tagtagttag aaacatttac
    24121 atgcaacatg gacagcgaaa ggatacactc atgcatcaca tgcaaaaaat atggattggg
    24181 acaggaactg ttgccgcggc gattctggga ttgtcgcttc tgacaactcc tgcgtatgca
    24241 gatgaaacaa tagaaacgat cccgccggtt gccaccgcaa caacacagtc ggagcagttg
    24301 actacaggct gggtgcatca ggcggacggt tctattattt actatgatac cgatggcaat
    24361 aagctgactg gcacacagga aattgacgga aaatattacg tgttttcgga aagtggtgta
    24421 ctgcttaccg gctggcagac tgtcaatggg aatcgatact actatgacaa agaaactggg
    24481 gaatcacagt tcggctggct ggactggaac ggcagccggt actacattca agaagacact
    24541 ggtaaacaaa ccggttggct ggttttggag aataacaaca tttcccgccg gtatttcttc
    24601 aatgcagacg gtgttttgca gaccggtttc tttcgtgcgg aagaaggcag cccgatctac
    24661 tacgccgaag cagatggtgc agtgctgaaa ggcgggatcc atgagatcga cggtgctccc
    24721 tactggttct atgaagacgg agcgttgcac actggctggc agacagttga cggtaagcgg
    24781 tatttttacg atccggaaac cggtgcgatc acgttcggct ggatcaactg gaacaaccgg
    24841 aattattata tttccgaaga aaagggaaaa tatactggtc tggagcaggt agacggagaa
    24901 tattatccgt tctcggaaga aaacggtgct attcaagagg gcatgcagaa gatgccggat
    24961 ggcacgactc gctattatta caataacggt gcattccaca gaggctggtt ctatcagaac
    25021 aaccagacat attattttga cgaaaaaaat ggttctatgg tcactggttg gcaaagcatt
    25081 agtggtgcca cctattattt tcaggaagac ggtgctgcaa agactggctg gctgaaagaa
    25141 aatggcaagc tgtactattt cggagcagat gcccagcttt gcactggctg gcagacaatt
    25201 gacggcaaac gatattgttt ccggacagac ggttctgcgg tcatcggctg gcaaaaagct
    25261 gccaatggtt tcagctatta ttttcaggca gacggttcca tggcaatcgg ctggctggac
    25321 attgataaga acagatatta ttttcagaaa gatggcattc cggctcatgg tgtcacggtg
    25381 attgacggaa agtcttacct ttttgcggac gataccggca ttctgcaatg cagtaaaaca
    25441 gtaaatggta ttaccactga tagcaacggc gttatcacaa agcggcagtt gtcagtaaaa
    25501 tatatcagcc agagtggatt ccctaccggc tgtgaaagtg ccagtgctgt catgctgttg
    25561 caaaacgccg gctacaacgt cagcgttgcc gattttgtgg ataagtatct ggataagggc
    25621 ggctttacat ggaaaaacgg tatacagtac ggaccagacc ccaacagcaa gtttgtggga
    25681 gatccgcgtt cttccagcgg gtttggctgc tatgctccgg tgcttacaaa agccatgaac
    25741 aagattcttg gaaacggcaa gactgcaaag aatctgactg gtactgcttt cacgtccctg
    25801 ctgacggatt atattgacaa tggtattcca gtggcggttt gggcttcaat taacatggta
    25861 aatgtggaca acggcagaca gtgggtcatt ccggaaacgg gtcaattgtt cacttggaaa
    25921 cggcatgagc attgtctggt gctggtaggc tatgatgcgg aatattacta catgaatgat
    25981 ccgtaccaga gcaagggact ggttgcctat aaacgcagcg tggtggaagc acgatatgca
    26041 caaatgggct atcaggcagt tgtcattcta taatcatgaa ccacagcaca taaggcaata
    26101 ccgcacaaag agcgtctggc gactttgtac gccatctgct tgcaaatttc tcgtcatatt
    26161 tcacaaaaat gctcgtgaat acatcaagta ttcactccgc tttttgtttc atctgacgaa
    26221 aaatttgacg acgcatctgt cgcacaatct ttgtgcggta ttgccataaa aggaggtttc
    26281 tgatgaaaat tttattgatt cgtcatggtg atccggatta tgaaaaagat tctttgacgg
    26341 aaaaaggctg gcacgaagca gaactgctgg cacagcgtat ggcagaactg aaaattcaat
    26401 cgttttatgt ttctccgttg gggcgtgcca aagacacggc ttcctgcaca ctgaaaaaaa
    26461 tggggcggga agcagaagaa ttggcgtggc tgcgggaatt ccatgcaccg attccggatt
    26521 tccacacggg agaacctcgt attccgtggg atcagcttcc tgctgactgg accgcagagc
    26581 cgaaatatta tgacaatatc cgctgggcag aaactttgcc aatgctggaa ggacatgtga
    26641 tcgatgaggc gaatcgtgtc tggaatgggc tggacgaagt actggcaaag cacggatatg
    26701 tccggaacgg caattattat gatgctgtat ctcccaatga ggatacattg gcgtttttct
    26761 gccacttcgg cgttacatgc gtcatgttaa gtcatctgct ggggatttcc cctatgatct
    26821 tgtggcatgg cttctgtgcg gctccaactt ctgttacttc tctgattacc gaggaacggc
    26881 gaaagggtgt ggcactgttt cgcatgaatg ctttcggtga tacggctcac ctgtttgcca
    26941 atcatgagga gccggcattt gcagcaagat tctgtgaaac ctttgccaat caggaacagc
    27001 ggcacgacta aggaaacact gaataaagct tgtgcgtaag atgcgtagtc agattttttg
    27061 gcagattgcg tgcaaagccg tcaggctggc tttatgcagt gtttccctaa gactgccctg
    27121 tacaacgtat atcacataga gtaatttaca gctgttattg tctgttcttt caggcggcaa
    27181 cagctgtttt tttacgcaat ttccggcaaa tacagacttt tttcaaaaac ctcttgacaa
    27241 atccacggat atagtgtact atatagctag tgaactagtc acccagtcaa ttggaaagga
    27301 gcaggcaaat gaactttcag gcaaactacc ccatttatct gcaaatcgct gaagacatca
    27361 agcgtcgcat tgtcatcggc gaattaaagg caggcgataa actgccgtcc aatgcagaac
    27421 ttgctcttgc ctaccaagta aaccccaata cggtacagcg aatctatcgc cagctggagg
    27481 cagagggcat ctcctatacc aaacgcggca tcggcacgtt cctcatggaa aatccggaaa
    27541 tgccggagca gctgcggcag gagatcggcg agaaactaag tgcacaattt ctcgcccaga
    27601 tggcaactta tcatttttcc aaagaagaaa tcatacactt gctggaagca caggcacagc
    27661 aacaggagaa agaaggcaca tcatgctgaa atttgaacat gttacaaaag tttacggcgg
    27721 gaaaaaggct ctcaatgatc tgagcatctc tttccagtcc ggtcatgtct atgcactggt
    27781 cggtcccaac ggcagcggaa aaagcacaat gatgaaagct gctgccggtc tggtaaaggt
    27841 aaacaccggc agcgttacct atcaggatat gccgattggt gtggaatcca aaaagcatat
    27901 ttcttacatg agcacggagc cattttatta tgattatatg aagattcgcg atgtgggaca
    27961 gttttatgcg gactttttcg aggatttcga cgaatctcgg ttcgcttccc tgctgcaatt
    28021 catgcagctg acaccggatc tgaaagtcaa ggcgttgtcc tctggcatgg cggcaaagct
    28081 gaaaattgcg gcaacacttt cccgcaatgc ggaagtctgc atgttggacg aaccgctgaa
    28141 tggcattgat ctaatcggac gtgaccagat cattcagagt atccttcgtg ctgccaatcc
    28201 caacgcaacc attctgattt ccagtcacct gtttgacgaa ctggaaccga tcgtagacca
    28261 tatcgttatg atggctaacg gttctctggt gctggagggc gaactggaag aaattcgcac
    28321 acggtatggg aagtcgattt ccgatttgta tcgggagtat ttttccggtg tttcgcagcc
    28381 ggcatttccg cagaatccgc cggtcaatcc gactgtataa ctctataacg taaaggagaa
    28441 tttgattatg ggtactttgt taaaatatga attccgccgt tcccgtgtga tttttctggg
    28501 gattatcggc gtaacgcttc tggtagaact gctgtatctg gttggctggt gcttcaagct
    28561 tgaaacacca ctgcttctgg gaatcatcgg tggcgtgctc tgtctggcat tcggggcaat
    28621 ggcaatcttg ctctatggag ttatcatgtt caacgacgac atttccaaaa agccgggtta
    28681 tctgctcttt tccacaccgc ggagtgctgc ccagatcgtt ggagcaaagc tgctcatgac
    28741 attgctggca ctgttgggga tcacagttct gtttaccctg ctgatcgcag tggatgtctt
    28801 tctggcattg cagagaaatg ggacttcggt tgttgctctg ctgtccatgt ttgatgcttc
    28861 gatcacagaa agcggtctga aagatgttct gttcaacggc tataactttt tcggattggc
    28921 aatgtacatt ctgaatatgg tgatctcgtt tattttccag gtcattgcag catatgttgt
    28981 gattactctg ctgaaaactg taatgggcaa tcagaaagga cgcaccattc tggcagtgat
    29041 tctctggttt gtgattatca atgcggtcag catgttgggc ggactgttca caacgcatct
    29101 ggcttctggt ctggataacg aaggaaaaat tatgttcaca caggaaagtg gactatccag
    29161 cacagtgttc ttgggagatt ttctccacgt gttgtttcag ccggcaatgt atctgccgac
    29221 tatgctgctc tgcattgcag gctctgtagc gggttacctg ctgactgtat ggatgatgga
    29281 aaagaagctg agcgtatgag tatgcacaaa cgcaaaaagc cgattctgcc ggaacaggaa
    29341 tttccggaga aaaaatgcgt tcttatcggt attgtcattg gtgtatctct cgggctgctg
    29401 ttgggcgtga cttgcggaag cttactatgc ggaagtttgc cgttgggaat ctgcattggc
    29461 ggagccgccg gattttgtct ggggcttgcc gttggtacag catttcagaa aaaacaatcg
    29521 taaatcagaa caccgcacaa agctgctgaa tttgctttgt gcggtgttgt tttatttggt
    29581 cagataatgt tcgtacaagg gaatggcttg gtgaatggca aaagtggtgc gataccgata
    29641 ttctgccaaa tccgactgca tctctgcaat ggcggaattg atgtatccga catgttcctt
    29701 ttcttcctcg tagaatgtca catgatcgcc ctcgctggtt tccgctgcct gcagagcaat
    29761 ttccagatta cagtcattgg attcggcata atccaaagcc gatttttctg caccagtctg
    29821 cacttgtgct accagtttct ctacagcaga agcactgtcc cgatatgcca ttaaaatggt
    29881 gttgttggaa attttcgtaa ttgtatcgta gacgtttttc actgtaccgt catcatcagt
    29941 ataggtatac cgcggaatcc agaatggcag acaataagac actgacagat tttttgaatc
    30001 ggcatatttc tgggcagttt ctataaattt tatctgttgg tatcgggcac tgtcattgtt
    30061 tttccagtta aattcggaat tcgtccagac ttctacatca taggaaacac cggcaattcg
    30121 agcatctgca tcgactaatt ggttgtattc ttctacacgg tcaaagacac ggtaaatggc
    30181 ggtctggtaa gtatcttcat acagccatgt tttttcgcca gtcagcaggt aaactttcat
    30241 gttgcgggcg taggcattct ttacaaaagt tgctaccatg tctgcattgt tcaccagttt
    30301 attggcacca atagagtagt agatctccgt aatgccgtaa aattccatgg catctaagaa
    30361 ttgattctgc actgtcgtat tattatcgat tccgtaaagc gctgccttgc tgcccttcca
    30421 gtaccatgtt gcccgattca tgttggtttc tgtattgacc acataaacat ccttggttgc
    30481 ggcattgtac agatattgtg tggtgtctga taagccaata gcatcggaat gtaccagata
    30541 gaatcgatcg accgaataat ccagcggacg gcccattttt tcctcaatgg cacttttcag
    30601 ttctcctgcg atttgatagt tgtattccga tttggaaata taaccgttct gaacggcatc
    30661 agcatatgtc atgccaacca gtttttccaa tgggctgttc tctgccggcg aaaccaacgc
    30721 ctgtcgcatc aaacaaaggt cttgtgcgtc cagcttttcg tctgctgaaa aatctcccgc
    30781 ctgccagtcg gcaagctttg tgtttgactc tgccaaaagc cacttttgca gagaaactat
    30841 atccgctgtg gaaaatttgc cgtctgcgtt gacatcacct tttccctctg tcgcagatac
    30901 cggcgaatat ggcatggaat aggctgtcaa caggaatgcg aaaatcatcg ccagtgtttt
    30961 tttcatagat tttatttcct ctcaataaaa taacctaagg caataccgca caaagagcgt
    31021 ctggcgactt tgcacgccat ctgcttgc
//
LOCUS       DAWBGR010000006        30831 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_575_length_30831_cov_4.282623, whole genome shotgun sequence.
ACCESSION   DAWBGR010000006 DAWBGR010000000
VERSION     DAWBGR010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 30831)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 30831)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..30831
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_575_length_30831_cov_4.282623"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3340..3439
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6506 a         8070 c         7389 g         8766 t
ORIGIN      
        1 caacatttcc gtgatgcatg ggaaatgaaa tataattttt ttcctctgtg ccatagtatt
       61 gtaaatactc tgctagtact tcccgaattt ttgagttttg aatgggcttt tctaaaaaag
      121 aaaatggatg tactgcaaaa gatgctgcca tttgctctga ataattacta acatatatca
      181 gagcaacttt tttatttttt tgtcgaatca atcttgccgt ttcaattcca tcaatacctt
      241 ccatttgaat gtccaaaaat atcatatcga aatcagtgct ttgcgtaagc agttcttccc
      301 cctgtgtaaa acaagaaacc ataatgcttt tccccttttc atgaaaaaag ctttgaatga
      361 tttttttcaa ctgaaaaatc tgatctttct catcatctac caccgcgatt cgaatcacgc
      421 catctacgct cccttcgaca cattcttcct catttttatc ataccacaat tttttccaaa
      481 aagcaagaca cctttctgct tcaaaaacag aaccgcacac gctcatacgc aaaaagagcc
      541 acaccctttc gagtgtgact ctttttctga tgccgcccag agccatctga gcatgcagtt
      601 actttttacg ccgctttgtc aggattgcca gaagcagtga gaatccgcag gctgcgatca
      661 aaatggtgat gtgcagtgtg gtatctccgg ttttcggcgt tcccgaaatg ttttgctgcc
      721 gcggaagcgg tatccgcttt gttccagaga tctgtgcaga aacatctgcc gccgttgttg
      781 tcgtctgcgt gtgatgacct gccgcatgct ctctgtttgc agctgttgtg gtagtcgtga
      841 cagtgtcccc gctcacgtga tttttccgca atgtggtctg tcgaatgctg gtggaagcac
      901 ccgatgtttc gcaagtgctg ttcaccgttg tttcagtaac tgtggtggtt tgtgtcgtgg
      961 tatgcgtgct gtggtgtgtg gtctgtgttc cagtcgatgc actgctgtct gtcgttcccg
     1021 tgctggtggt tccagtcgtg gaaactgtcg cagaagtggt gcggctggtc ggtgctacag
     1081 acgtggtatt cgtctgagat accgtagttt gtttcggcgt tgtctgggta gtagtttgac
     1141 tggtggtatg tgtcgtagtt gtcacgctgg tcggacctgc actggagagt gtcaccgttg
     1201 ctgtagtctg ctgtgttgtc gaagatgtcg tgtggtgtat tgcggaagta gtgactgacg
     1261 tgctgccagt agtcgttaca gtagctgtcg tgtgagtcgt tcccgtggtt cgggaagtcg
     1321 tcacagatgt tctctgggta gtgatactgg ttgtgccggc agtcgttctg tgactggtgg
     1381 tatgtgtcgt gtgcgtggtt gtttttgcaa tcgtatgtgt tgccggtgat gcggtgactt
     1441 tcgctgttgt tgtgtgtgat gtgctgtgag tagtctgttt cgaggttgta acgctgctgg
     1501 tggtttgttt catagctgta gtatgcgtac gtgtggttgc agtagtatga gacgactgcg
     1561 ttttcgctgt agtccgtgtt gttgtggtgc tggttttcgt agtgcttgct gtagtccgtg
     1621 ctgttgtggt gctggttttt gtagtgcttg ctgtagtccg tgttgttgtg gtgctggttt
     1681 tcgtagtgct tgccgtagtc cgtgttgttg ctgtgctgga ttctgtagtg cttgctgtag
     1741 tttgtgttgt tgctgtactg gtttccgtag tactgctttc cacagaggta gttcccgcag
     1801 atgccgaagt actggtctgt gtcgtcgatt ccgtagaaac tgcggtttcg gttgttgtcg
     1861 taacaagatc tgtagccgtt gttgtttccg tagattcttc cggatcacga ttcagaaaga
     1921 cattcagaaa agccatatcc tcgcccttgg cgatgttgaa tccttccaga caagttccgg
     1981 acgtgacgcc cttttcttct ggaattccca gaatcataca gccgtccaac gtgcgttcct
     2041 ctgcacgaat ctccacactg cccgacgata tgtcagcata ttcttccgca ttgtatcctg
     2101 ccggaatcga atcgatccac aggcggtatc ctgccagata tgtgacaccg tccaccgtca
     2161 cagacagcgg caagtcgtcg aaacgatatt gtccgtctgc attggtatag gcaacttgtt
     2221 ttgcttcagg cagcagttcc cactttccgt tgcggcgata atactgccgg agtatgatct
     2281 tcacattggc gataccggat tcgccgtcgc tgatctgatg atctcggtct gcatcatccc
     2341 agataatacc ggagatcgat acatgagaat ccttctgcac aatgccgccg tcatatccgg
     2401 tgacatctgc cgcctttaca atgtcatagt ccacgccgcg aacctgacag caatacggtg
     2461 tatttccgct gaatgctttc gccaggatca ggaatgtatc cggctcctgc agatccagtg
     2521 tgccggcgat cagatcgctg tccttgtcgc tgtcggcagc cgcatgatat ttcgttgcag
     2581 catatccttc cggcatctca caaagctgca tctgataccc cactaaatat ctctgaccgt
     2641 ccttttctat gtaggtcggc agattgtcaa agcggtagtg tccgccagat gcggttgccg
     2701 ttggtgtgcc agttgccaca ggtgttgctg tcgctaccgg tgtgcctgtt gcaacaggtg
     2761 ttgccgttgc aactggtgtg ccagttgcca ccagcacaaa gctgtcgtcc agtttccaag
     2821 tggtatcgcg gctgtcgtaa tagcttcgtg tcagcttgac cgttgcagtt gcaacgccca
     2881 cttcgttttc gtcctgtatg ccgttgtagt tctcgtcatt ccagatgtag ctttcaatgg
     2941 acgcagtttc gattgcagtc agaccgccgt catagtccgt gttgtccttt gccttgacaa
     3001 tgtcataggc attgtccccc acgttcctgt cgtagaagtc gttgccgctg gacgcttctg
     3061 ccagtatgat cacttcgtct gcatcttcat acagatgtaa cgtatccgca aacagatcgc
     3121 tgtccttggc tgtatcctct ccgattcggc acttggtcgc tgcgtagccc ttcggcatct
     3181 cgcaaagctg taactgataa ccggcaagat aacgctcgcc gttccgttcc acaaatgtag
     3241 gcaggttgtc aaatcgataa tagccgccag atgcggaggc tgtcggtgtg ccagttgcca
     3301 caggtgttgc tgtcgctacc ggtgtgcctg ttgcaacagn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnna tcggtaatag ccgccagatg cggaggctgt cggtgtgccg
     3481 gttgccacag gtgttgccgt cgctaccggt gtacctgttg ccacaggcgt tgctgtcgct
     3541 atcggtgtac ttgtcgctgc cggtgtgctt gtcgctaccg gtgcaccctc tgcaaccaat
     3601 gcaaagctgt cgtcctgctt ccacgtctta tcagaatcat cataatattt ccgtgtcagc
     3661 ttgaccgttg cggttgcaac gcccacttcg ttttcgtcct gtatgccgtt gtaattctcg
     3721 tcattccaga tgtagctttc aatggacgca gtttcgattg cagtcagacc gccgttgtgc
     3781 ggtgcgacag atttcgccag atttgccggt gcatatgtgg tacctgcaat ggtaatggta
     3841 tgctcccgat ctgcatcagt tgccttcgcc gccaaaatga tgtgcgactc gtcctttgcc
     3901 agcagactgc cgtcacgggg attcaggaaa ctgtttttca gagcctgctc tgattccgca
     3961 tactgcttgg tcaccgcata ggtatccggc acagttgcca gatccagccg gatctgatag
     4021 ccatagatgc gatccttgtt gccagaagtg ctgtcatctg acaggtctgc cggcacgtga
     4081 acatccagat ccgtaaactg atactcgccg tgatccacaa tttcagaaac ctcaccaaat
     4141 gcagtatccg gtatccagtt tccgttttca tagatctcac gggtcagtat cacgcgaacc
     4201 gaagttgcca ccggcacttc gttggaatta tacaaaccgt catagttcgc atcgttccaa
     4261 atggtaccgc gaatcacgcc cttgtcctgc tgcttgagtc cggcatcata atgtccggta
     4321 ttcttgccgc atgcaaggtc tactgcgtac atggtaccgt tgctgtccgg aacttcccgt
     4381 acagaagcac cattctggtt tccgttggca tcgttatacg catttgccgc cggaacaatg
     4441 tattcgttcg cactcgtcag atatgtgctg tccagtcgga ggtcgctgtc cttggtgcgg
     4501 tcagttccca cacgatactg tgtcgccaga taattgccgc gaagattttc cagacagaac
     4561 tgatagcctg cggcaaaatc catattgttg attctcaccc aggtgggcag attcgccagt
     4621 gtgtatttgc cgtctgcatc tgtgcttgtg ctggctgtat acgccgtgcc gttcggctgc
     4681 cactggctgc cgtcataata atactgtatt gccttgacgg taacatttgc aattgccgcc
     4741 ttgtcctctg cggtattcgc cgcatcatct gcttcgtcct gcatgccgtc atagttatgg
     4801 tcgatccaaa tccgtccgga gatgtcccct actgccaact gtgtaaagcc ggcatcataa
     4861 ccggaaacat cttcctttcg gagaatatct ctgccgtcca ctgcataatc cgtgctgtct
     4921 gtatccgcag ctgcacaaac aaaataggtg tctttcgtca gataacggct ctccgcatcc
     4981 agatcgctgt cccttgcggg atcatcgccc tgccggtacc aggtaatggt gtaattgtcc
     5041 ggcacttcgc ttgtcatttt cagctgataa tagcagaggt aggtttcgcc atttgcatta
     5101 cagaacgtat ccacattgtc aaaccggtac tcgccgttgg catcagaatt cttctggaat
     5161 atgccggtag gctgatagtt gccgttggca tcgcggtagt actgctccaa cgtaatttct
     5221 acgccggaaa tgtttccctc gccggtatcc tgcaagccgt tataattcgc atcgtcccag
     5281 acacgtccaa cgatctgtgc cttttcgacc tgcttcaaac cgccgttcca gtctgtctgg
     5341 gctgcggcat ctgcaatgtc atagtcactg ccgttgtagt gctttcggta tgtcgaatcg
     5401 gtttctgtct ttgcctcagc agccagaatc agatactgat ccggtgctgt cagatagagg
     5461 ttctcgctct gatacggatt ttcaagtctt tggaatttgc tgtcgccgcc tgccttggta
     5521 atgccgtagg tgatcttacc gtcagcgtcc ttcggcagtg cgtccagctt cagccgatag
     5581 ccagccagat accgctcgcc gccgatctcc acaaatgttg gaacattttc gaaatgatag
     5641 tccccgttgc tgtcggtgtt tacagttgca gtgtaatttt gctgtgctgt cggcagccat
     5701 gtggtgccgt cccagtaata ctgctccagc gtcatcgaca agccttccat gccagcttct
     5761 tccgccgcca tgccgccgtc ataatcggca tcattccaga ttcgtccgct gacagacgtt
     5821 gtctggaacg cccgaacgcc gccatggtac tcgttcttgc tgtcacggct ttctgtgatg
     5881 tcgtagatcg tatccgtatt cggaactgca ctgtaagtgc ggtcgtaata cgtgttgtac
     5941 tgcggtgtgt ttccggtatg ttctccggta aacggctgtg cctctgctgc aacgatcaga
     6001 tattcctgct cgcccatgag agtgagttcg ccattcttcc agtgcagctt gttgtcgttg
     6061 gcaagtacag taactgcatt gccgtccggc agtgcatcta agtgcagctg atacccagcc
     6121 agataccgct tgccgccgac ttccgcataa gtgggaatac cttcaaaatg atacagaccg
     6181 ttgctgttgg tcactgcggt catagtcgcc gcagtctttt tccagatcgt tccgtccaga
     6241 taatactgat acagtgtcaa atggagatcg ccgcagccgc tttcgctcaa atccattgta
     6301 ccgtcataat tgaggtcgtt ccagattcgt ccgtccattg ccgcagtctg tatggatttc
     6361 agtccgccgt tgtatacgcc gtcagaatct cttgcggtaa caatatcata atcgacattg
     6421 tcgtacttcc gcacgtagta ttcgtggaat cctgactcct tgcttccggg tgcaccgtga
     6481 acagccttct ttgccagcaa gatatactcg tccggctggt tgaagtccac ggtctgatct
     6541 gcattcgttt cctgagatgc tgtcagcagg tcactgttga tgcagcttcc tgtgggatcc
     6601 agcacctgat actttgtcac agcataaccg tccggcaccg cgttcagccg caggcgatag
     6661 cctgccagat acatctttcc gtcaaccgtc acaaaggtcg gctgcttgga gaactgatat
     6721 actcctgtgt ttgccgcagt ctttgcggag agtgtctgat gcagcttcca gcttccggca
     6781 tcgtccagat aatactgatc cagatagacc gtctgctgtg tcgccattgc cgtttcaccg
     6841 gtttcctgca agccgctgta attcttgtcg aaccaaacca cgccctgaat ctttccggtt
     6901 tcgaacgctt tcagaccgcc gttataaatg tcagtcacgt ctgcattcaa tatggaatcg
     6961 tattccttgt ttgtgacagc atcagtcaga ttatagggag cgttaccttg tgccggctgt
     7021 gctgtcagga agtaatctcc attgtccggt gcagtcagat aattcgtttc tgcgatccag
     7081 tcgctgtctg ttttcgtgtc atctgttgcc tgacgatacc gtgtcaccgc atatcccgcc
     7141 ggcacagccg cacgaatgcg gtaatatgcc agctgatctg tgccgtcaac cacaaagtgg
     7201 gtcggcacct tggcaaactg atatacgcct ttggcatcag ttttcacagt catgtcattg
     7261 ttcatgtgaa caaactgctt tacggcagca tcatagcgat actgttccag agtgacagaa
     7321 acgccgttct tgcccttttc accgctgtcc agaatgccgt tgtaatccgt atcttcccag
     7381 atcctgccct ttactgccgc aatgttgcgt tttttcagac cggcattcag attttcataa
     7441 tctcttgctg ttaccagatc atagtacttc tgctgtgcag tattcggcgt gtcgatattt
     7501 ttgatgttcg tgccgcagac atatgcccag ttgtaatccg tatacggttc tgcatcgttg
     7561 gactgtgtgc ctgcctttgc ctcccgtgcc ggaatcagca tttcgtgatt gccttgcagc
     7621 acagtaaccg gcaggtcacc tgtccgctgg ctgtcctcgg caccatcggc atcggtcaca
     7681 tacagtttgc tgtaaacctt tgacggatct gcatcgttcc agtacttcgt tgccgcataa
     7741 tctttcggca ttgttttcag gagtacctga tagcctgcca gataccttac gccgtccttt
     7801 tccacatagg tgggaagatt gctgaacgtg taggttccgt ctgttttggt ttcgtgtgtc
     7861 cggaatgctg cattttccag cacccattcg ccgccgcgat agaaatactg ctgcaagttt
     7921 acaactgccg gattcttgct gttatgatat gctgcttcgc cgtcgtcctg aataccatta
     7981 tagttggtat cctcccagac cacgccgctg acagaggcgg tttcaaatgc tttcagaccg
     8041 ccgtcataac cgttataatc ctttgccttg atgtaatcgt aagcataatc atgatttccg
     8101 ccgtgcactg ctgcatcatc atagagaatg tacggttcaa aataggtgtt tccgtcaatg
     8161 ccagcttctg tttccggctg ggacttcttt gcagtgatga aataatcacc gtcagccgga
     8221 gaggtcagat aagacgcaat gtcataatct gcattgcctg ctccgtgctg tccgttctgc
     8281 gagatcaggt ggctgtcgat cacttgggtt tccgcatcgc cgttctgccg atagcgggta
     8341 acagcatagt tttccatgcc gctgggcaac gacagcagct tcagacgata atgtgccagc
     8401 accttttccc cattgatttc cgcatatgtg gaaacaccgg agaatgtgta cttgccgtct
     8461 tcatcagttt tcacagtacg gtttccgctt gttaccggta cataggtacc attttgcaga
     8521 acatactgtt ccagctgaac agtcgctccg gcaattccct tttcatagaa ttgtgtcgcg
     8581 ccgtccgcat cggtcacggt attgcggata ccgtcatagt cgaatgcctc ctgatattct
     8641 ccggctgcat tcctaccgtt gtcctcccac agataaccgg ttacttcaga agtctgaatc
     8701 tccgtaaagc cgccgctgta gtccagcaga cgctgtgctt cgccggtatc ataatgggtg
     8761 ttcttatagt cgatgacgtt gctgttcgta tttgaagtat ccgacgtttc tccggcaaca
     8821 atgatgatgt tagcgtctgc atcatagttg tgatgctttg ccgtcacagc accattctca
     8881 tcatactcga accagtcgtc gccaatgagg tagtactgca tctcgccggt atactgcaga
     8941 tcactgtttt ccgcagagcg gctgtttgga tactgcttat acttggtgat cgcatacttc
     9001 cggtacagat cggcattctt atccggatcg acgtacagcc gatagcccgc cagatatctc
     9061 tttccgccct tctcataata gctggaaaca ttctggaata catatgcgcc ggattccgcc
     9121 gtctgaatgt ctcggtctcc gttggtaatg tactcccatt tcttcgtgtt cacatcgtaa
     9181 tagaatgacc gcagggtaac cgtcacattg ccgataccct tttcataggt atcccggatg
     9241 ccgttgtagt tggcgtcatc ccagatccac tgtccgacaa agccgcggga accattcaca
     9301 aatcccagag aaacactctt gttgatctgg aatgtctgtc cgtcgtcttc atacagcggt
     9361 ttatgatttt catcataagg aacgctcgtc aggaacgggc ttgtgcggaa gctgcctgct
     9421 accttcatca agtcattgtc attatccttt ttcagcggat catcatacag cataaagggt
     9481 gacggcttat agggaacacc ggaagttccg tcataccttg ccactgccac atagttctgg
     9541 ttcggataca gacggaactg ataatagcct tcatcactgg tgcggacgac tgcccgattt
     9601 ccgtcaccgt ctaccgcaat ttccgatgcg atgttgccgt ctgcatcgac ttcgtgcatt
     9661 tccaccgtca catctttcag cttctgttcg gcttcctgca tgatgccgtc gctttcgatg
     9721 gtgccgttgt tgtctgtttc atcgacccat gcccagccgc cgtaagtgta tcctctggaa
     9781 acacccagca tataggaagt catggcggtg tcatatgcct gatgctttgt cagatcttct
     9841 tccaccgcat ctacatgaat cagtgccgtc tgtacgatca gctgtccgga cgaagccttt
     9901 gtgttggcaa tttcgttgga aactgccgta tggtcatctt ctgtgcgaat gccggtataa
     9961 acgacatttc cgtcacggta tactttcagt gctgtgccag tctgtcccag ttcccgtgtt
    10021 gtaaccgagt actgatcgta tccctgcggc agtgtatacc gcagccggta atctcccggt
    10081 gtcagattcg acagcatgaa gtagccctga tttccgtaat agtcgctttc ggtggtataa
    10141 gtcaccggtc cgtcaaacgt atactgatag gaattggtgt tgttttctac aatggttttc
    10201 ggctgaccag tttcctcgtc aatgacaaca taacgtgtct tttccggatt cgggttgctg
    10261 tcaggatccg gatcggcaat cactgcctca ccgttgacgt tgacggaata gcccttcttc
    10321 gtcagcagtt ccacctttac gccattgata cccggatcgt ccggctttcc gtcaaagtca
    10381 atatcggtcg tccatttgcc ggtaggcagc aaccgtccgt tggcagacat ctcatattct
    10441 gcttcgttac actgagcgtc atagttgagg tcgctccaga catagctgcc gatatagccc
    10501 tttccgctgg gtgcagaaat ataggcaccg gcctgtgtat tttcaaagac ctgatagttc
    10561 ttgccgatcg cacttgcaga atcatagccg gaagcaaagg tgttccattg tgtcacaccc
    10621 tctacctgct tgcgaatact ctccgcagat gctgcacttt cattctcgtc ctcagttgta
    10681 ggtgtaccaa catacttcgg cagattcagc ggtgcatgca tctcatagta gagttccata
    10741 cggttctttt tgtccagtct ggtgtcggca ttattcatct gtgcccagat cgcacggatc
    10801 ccccgtacca gccgatcgta ttcggcatca gagatcctgc cggcttcgtg atcgccgatc
    10861 agttcagaca gcttgacaaa atacgaattc tttccgttta catcttgata cagactccgg
    10921 tagaaattgt ttttctcggt atccatcaga tcatctgccg gcttcgggag gaggctgaca
    10981 tcctgcagca catagctgcc gttcttcttg atgatcggtc cgacccagac ggtgtacata
    11041 gacggattca gatttactgt accgctggtg gatacttgtt tcagctgcac agaatccgga
    11101 atcagataac cgttccactt cgattttcgg ggtacgttcg aatcagttgc cttggtgtcc
    11161 tctgcatagg gcagtacatc atagaacaac agcgtcttat attctgtatc cgcatccgag
    11221 ctgttctgca tggaaatgga gtaagagtaa ttgccgccct cctgtacccg aaccggaatt
    11281 gccgccttta cggaatccag atcggaagtg gctgttttca tttgtgtaac attatcgatc
    11341 gaaagcaggg tgattgcctg tgatgtgatt gtcagtgtca tatcgccgct gcttttgttg
    11401 tcgttgacat catggctgtc tacttcatag gcaacgatct tgacttttcc ctgcttatcc
    11461 ggaatatacg gccggaaaga accgttttta aaaccatatg cctttgcctg catcagttcc
    11521 gcagaaacga tgttactgtt gctgatgtcc agcggcacca tgagttctac cacaatacgc
    11581 tgtcccggct ccagagttcc ggagaacttg aagttgacga ttttccggtt cagtacattg
    11641 gtggagatcg gttttgtcac aaagtagggg ttggggttct tctgcgtgtc catagcgact
    11701 tcggtgtttg tcacgtctgc atacgggagt tcttccacgt ctacgtttgc cagtgaaaca
    11761 tcaccgctgt ctttcaggga aacaaattca cccttttcgt tttcaatatg ccatgtccac
    11821 tgcggtgtca catcgtccag attcttcgca ggcgttacca cttgattgtc gctgttcttt
    11881 tccgcagtgc tggtgtagtg atcgccgacg aaatagccgt caatgctgcc gttggaatcg
    11941 tcctgatact gcacatactg gaatgcgtcc gcagagagcg attccagata cggcagtgcc
    12001 agagaaaccg taggatcaga cagcttgtct gcctctgcat ttttccggtt atacgtactt
    12061 gtgttcagca gctcctgact aagattctta taagtcactg tatatttcag catcttgccg
    12121 gtaccgctgg aaataccgct ggtattggaa tcccatttgt attccttgtt tgcagcaccg
    12181 ctgaaatact tggcatcaat atcgataccc agccacggca actgcggatc cagatagaag
    12241 cccactctgg actcatcacc ggaaaccgca tacttgaagt catcgctacg caggatcacg
    12301 ctggcgaaat ggttgttgtg tacagaattg gagatagacg ggtcattcgg tgcagtggta
    12361 aatgccacat gggggctgtt ttccttgatg ctttcgctca ggggttccag accatagtga
    12421 tacttgtcca catcgttgcg gtcctgtctg ccctctgtgg tgtcgtctgc atctgcattc
    12481 acatacaatc ccttcggcac cggaaaatat cggggtgcct ctgcgttggt cagccgaacc
    12541 tgagttccgg aaatcgtttc cgtaaagccc tctgtccgaa tcacccaacg cacctgacga
    12601 atcgatttgt gcaaattgat gctgccgctt gtgatctgct gcttcagcgt ttcgttggtg
    12661 ctgtcaaaat gcaggttgcc ggcttccagt gaaatcgtct ggtttgcgtc cagtgcatag
    12721 ccctgttttt cgccgatgcg tacccaccag ctggttccct tgccgttgtt ggcacaggca
    12781 tttgaataaa tgccgtcgtc ctgctccgtt tccggtgcaa ggaatgcttc ttcattggca
    12841 gggttcgtgc tgatcagcac atacggatac actttcaggt gcttttccag aatgtcatga
    12901 accagctgcc ggtctgcggc atactcggtt tccagtgcat ctgctgacgg aatcgcccag
    12961 ttaccggtag aaatttccgt tacattcgga taaaccagct ctgaaacgtg aggcagcacg
    13021 cccggtttgt tgaccggtgc cacctttgtg gtaccacgca gcgttgcgta gtttgccaga
    13081 ttgtagttga ccacgaattc gtttaccgat gcggtatcgt tagacacctg atcgacatag
    13141 ctgacagttt tataaatctg cttaatatgc gggtcttccg caatgacaat agaggagttc
    13201 gggtcgttga cacgcacacg ctcatagttc agatctgcac gcaccgtggt tttcggtgca
    13261 gtaacagcaa ctttcgcaga agaagcggtc agatagatct cacccggcac aacgccgtca
    13321 aaaggttcga tctccggaat ggcattgttc tttggacggt tggtatcctt cacccgcacc
    13381 gcattttgca gctgcatgcc ctccatagtc tgtccggtga agttgaaatc ataggtatca
    13441 tatacaatgt cacttgccgg cacttcctgc cgcagtttct tttcatagac gatattgccg
    13501 tagtcgttgt agacatagcc gttctcgtcc tgcttcggca tcaggcagcc gccgcggatg
    13561 tagtacttgc cgtctgcatc acgttctgca aacagaccgt cctttgtaat ggcgaacttg
    13621 ccgtctgtct gttcgaacgt tgcatagacc ttggcataat aatcctccca gctgctgtcc
    13681 gtatccgccg gcagttccag gttgtcgatt cgtgtcttga tcttaaagtt cagcgtgcca
    13741 ttgaaatagc ccgccggatg acagcctgcc acataggtgt tgtcatttgt gcagtcgaag
    13801 ccgtcatctt tcggcgtggt gatctcgaat accagcgttt caccgccacg ctttgtggca
    13861 gaatctgccc gtgtcagtcc gtccgccgtg ttttctgccg gtgcaactgc tccggcaaag
    13921 taccgctgtg tcagctcaat gccccagccg gcagcctttg cctctgccat ggtaaatgtc
    13981 tgctttttcc ccgacttatc ggtgtattcc agatagtagt cgtccagcag attgtcctcg
    14041 tcccagtacc gtacattttc cggcagctgg aatgtgacaa ccattttagc gtggaagatg
    14101 cttccgcggt aacgatagtc tgcctccgac tggctggaaa ccttggcact gtaccagaga
    14161 atatcactgt agtcaaagtt tttcccgttg ttctgatact gctgacgagt tttcacttcg
    14221 tccagatctt ccttgtttcc ggcaaagccc ttttcgcctt cttcggtctt ttcagcagct
    14281 accatgttct ccgcctttaa agacggagtc tgcgtgcgaa tcttcagcgt attcacaacg
    14341 ccggaatcct gcatcacatt ggcagaagta atatcctgga aaagcatatc actgctgttg
    14401 tcacgcagtg tcacatcgcg gttcagtgcg gaacgaattt cctccgacag cggcttgttc
    14461 tttcggtact catccatttc catggaatcg ctctcggcgt accggctcat gctgcccagc
    14521 atataggtac cgttttcgta cttatgggtc agcagaacgt aaggatccgg attggtggtc
    14581 gaaagtccgt cgttatctgt gtccggatac atgggcaggg tatcttcgga tttgtaatag
    14641 ttaacaccgc cataataatg acgagtcacc tttgcagttg cagcaactgc atcttttcgt
    14701 tcgtcttttt ttcggaacgc ataggtggaa tagtaattga taattggtgt aatcactgcc
    14761 gccgagcaaa ccttgtacgg atttccggaa atgtcattgt ccgtgataaa ccggtaagcg
    14821 ttcactctgc tggaaacata ggaatagttg ttcaggtaat tttccagcag gtcttcgtct
    14881 tccacgccgt tgatcacatc gtccggctct gcataagtaa aggtcttgat gcagaaattg
    14941 tacagactgc cattccgaat cgcagcgtcc ttttcccgct tttctgccag ttccgctgca
    15001 gtcattgcct tttcggtatc attttcctcc ttggcaaaga tctgcaccac ataacgcttg
    15061 gtagaatcat catagtagac ctcagtatca tagagtgcca tttgatctgt cagcttagtg
    15121 gtagcatcct tgtcgtacag ctcccagttc agaggcttgt tgtttttgtc gttgtccgtg
    15181 gtatagcgag agccatccgc cgcaaccgga acgatgccat agggcagaat gttggtgatg
    15241 accggcagcg agagcgaacc cttctgaccg ccgtctgtac catagctgtg cagcgatttt
    15301 tcgttgttgt acccttcagt accggcactg ccgctgtagc cgtactgatc ccagtaataa
    15361 ttattctcca catggacatt gatccagttg acctcgccct gtgtctcggt gtagtactgc
    15421 tctaccgtct tgccatacat ctcggtttca tccttaccga tggtttcgct gatggttgtc
    15481 cagctgcgta ccagaggttt ccgcagcacg gctctggtgc cggtttccgc gtagaacatg
    15541 gtcttaccgc tgctgtaacg gtcaatgctg tcattgttgg taaagcgata gccgctgccg
    15601 tctgctacga cttccttgtt ctggatattg tagccgttga cagacggcat attcggagag
    15661 gatgccaggt atttcagact tccaagctgt ccgtcgctgt cataccaata ccaatacagc
    15721 ggactccaga ggtagtccat gccgtacagc tggttgtgct ttgtcagcac cttggtggaa
    15781 ccgtcccagt ggtcgatgtc atagatctgg taatacagat gattctggct accctctgcc
    15841 acatagggct gggtgagaac acgatcgtac catgtgccgc ttgccgtatc ggtgtatact
    15901 cttgccggaa gtgtcacccg tgtgatatag ccgaagtcgc tgcccctgcc gtaccatttt
    15961 ttcagtgact gttttacagt gatcttcacc acatacgggg tgtattcctt tgcggtatag
    16021 tatccgtctg caccgtctgt gatgcttgtg ttctgtgccg catcccgcat tgcctttggt
    16081 gcaagatact gcggattttt gtctcccggt ttctgaatga tctctgcggt aacatatgtc
    16141 ccgtcgatct gggagaatgt atctgctttc gagtcgccgg tgttggtata cgccagtatc
    16201 tcagatgtat ccagcgtccc gtctgcattc atcaccggtt tcatgccata ggcataggta
    16261 tacaggaatg tcacgccgtc cagatcccag tcgccgtagt tgtacgcctg gagctgtacc
    16321 ttgacatctt catcatattc cagcgcagcc gcaccagacc ggtctgttac ctcatgtaca
    16381 ctattataat aggagtttaa tccatttccg ttggagtatg ttccgaaata cggggaattc
    16441 gggtcgcaaa gcacattttc cgtgtggtcg ccgttcgtct tttcatattg gatattgctg
    16501 gttacaaatt ccgaactggt ggcaagccat gccttttgca ggtggagacg ggtttcgctc
    16561 cacacaacag gtgtatcctt tgccgaatcc aaatctgccc agtctgcatg attcttgttc
    16621 cgcttgccca ttgccagctg gaaccaatct cggtttgcac cgcctgccat gtacttcacg
    16681 gtctgaggca gctgctggag ctggtcagcc gtttcgatca tggaattggc agtatatttc
    16741 accgtctgca tattctttgc agtctgtgaa tcctcgtcca acagcgtgtt gttctgtccg
    16801 ctgatgctgc cgcttgtagt ggaagaaccc ggaatgtggg taatggtctg atccagaaat
    16861 tcccacatat ccacctgtgt attcttatcc gcactgaagc caatatcgtg catcagatag
    16921 tccatatcca tctggatact gctgttctgc acctgcttga aattattcca gacgtttccg
    16981 gtatcagtga atccgccggt caacatcgga taaagtttgc cgttcgtaga ggaactataa
    17041 taattatgat aaatactgtt ctgataatac tcaccaatgc gggggaaata tgccgggtgc
    17101 agtccgtcac cgcctgcaac agatgcggcg gtatcgtctg cataggtgga cgcagtgtac
    17161 accataggca gttcttcttc ccgaatggtt gctgagtagc agaactcgat ctgttctccc
    17221 ggctgcaaaa cgaagtccgc attgggagca tctttgtaac gcagccgcag ttcgtatacc
    17281 gtaaaggaaa tctgatatgt ggagttttcg tccggcttca aatctgcaaa tgccttttgt
    17341 gttgcatttt cagaattgaa cccgtctgta taggtcatct ttccgccata gtcataccca
    17401 ttttgtgcgg ttttggctgc cagatacaat tctatattgc tcagatcttt ctcgctgccg
    17461 tccgcattca gcaaacgcaa attgggtatg ctgtcgccga ccccatacga atcgtacaaa
    17521 gtcttggtaa aatattccgg taccttatca tagatcaccg gattgatgag cctgccctcc
    17581 tcacctgcct gttttccgct gctcagtgcg gccaggttgt ttttcagtgt taccttctgc
    17641 caaacagtat cgcccggacg atagcttgtt ttctgtgctt tcttttcctc atacgctgcc
    17701 gcagcatcgc tctcggaaga aaatgtctgg gtgtggaacg atgcaatggg gttttcccga
    17761 taaaccttag gattcaggac tttggtgtct gtcaggtatg ccgtgtgctc tgtcggtgtt
    17821 ttggcagatg ccacactgct gccactttca gtcaccgtca ctgcattttc gttttcactg
    17881 tgaatgtgct cataggtgtt ggtggctgtg aaatactggg tataggtatc tgctctcacg
    17941 cctgtgccgt ctgtgacatc ctggtatctg ccgacaccgg tcagcgttac attttgcaga
    18001 gccgcttttg ccttggaagt accactgtac gcagtggtcg tatcaaagcc cttgagatcg
    18061 taatacaccc agcgaacgcc cacaacattc ggcaggtcgg cggctgtcat tttcgcagtt
    18121 gcgacttgat cggtggtgcg ggaagcatcg tagtcgtaag acttccatgt cacctctaca
    18181 ccgttttcgt tcgtccggaa tgcaatgttt tcgtcttctg caaaccgcgg atctgtaatc
    18241 ggctctgcaa cggcatagat cacttccact ctgccgaacc tgtctgtttc ctccggaact
    18301 gtgtagggct ccgtcagttc aaagccgcgg tagaacacat cgccgctatc cttggaacgc
    18361 ttttccagga aagaaacgtc taccgtgtag gtatcgcaat ggtgaccggt ttcattgtac
    18421 aggtcgctga ctgtcagcac ggcacccttt ccgtcgtaat tctgcttgat gtcgtttacc
    18481 tgataggaaa gcgttaccgg tgcagcagac tgataggtca gttcgttctg cacgtgtgca
    18541 taatactgtg tctgcttgtc tgcatctgcc ttggtatacc ggtagtaata agacacgcct
    18601 gcacggtcgg tcgttgtctt gttccagtag tatcttctgt ctgcattcgg cagatgtgtt
    18661 gaactggtgg tatcatagat tgcatactgc ggatagccgt gcttgggggc agcatgaatt
    18721 ttgagatccg gtgtgccgac agtgacagca tcatagctgc cgtcgttttc cgtcagctgt
    18781 gtggtcacac gcaggtaaac cgtttcaccc ggcttgatct ggtcatcgga ttttgcaccc
    18841 ttgcccagtt ctgccaccag ccgccggtat gcagtttctg ccggctctgc ttctgctgct
    18901 gcaagcaggg catacttgac accctgtgcc gcttcgatcg tcgtgccgcc gtctgacaga
    18961 gcagcgtgaa tatccgcagc cggaatattc tccgcggcat tttccgttgt cagcactttt
    19021 ccgtctgcat ctacaacgtc ccagcgaacg attcgctgtc cctttggaac agagaagtcc
    19081 actgtggtat gttccagcgg aatcagtgca tcgtccggcg tgttcacccg aatggtatac
    19141 tctacataat catacggcat caggtgcaga tagtccacct catcgatgtt tccggtcttg
    19201 gaatcctgat cgtacatctt tgtgcgggtg acttctgcgt tcagcatacc cgtcagattc
    19261 ttgactgtat agtctgcctc tttcttgctg ccctttgcag aaccgtccgt attcttataa
    19321 atataggtat tggcattggg gtcaatggag gtaaagactg ccgaaagctg gttggtggtt
    19381 atctgctccg gatagctgtt gggagccttt ccaaaggtcg gcgtggaatt ggcgttccac
    19441 tcgtcaccgg tgaaatcgtc cggtgtccgg tcgacataaa cgccgtcata caggaatgca
    19501 ctggtgttct tggtatgatc cgccgacagg tattccccgt cacacagcac agtactgctg
    19561 tcatttcggt ctgccttttt cgccttgaag tccagctgga agctgttgac atatgctttg
    19621 acataggttt tctgattagt cgtattggta taggttccga gaacgccgga atctacccga
    19681 tacagctgcc gcagaatctg ttccacatca acggtataga ctttctcgcc ctgtacggtt
    19741 tccgttgtgc tgacatgcac cacatagatg ccgtctgcca tgccgtctac gccggagcct
    19801 tttaccacat tcaccggaac gttcgtgctg cccacttgga gtgtcagatt tgccacgccg
    19861 aaccagttgt cgtccaaaag gaatttcggc aggtagatat gctccagccg gtatcggctg
    19921 tccatggtgt tggtggtagc cgcactgtca atacgtgcag ccgcctcctg cccagtcgca
    19981 ttctgcttgt cctgattgaa cagcttcacg cggaacaagc cgcggttcgg tgtgttgttg
    20041 tcgctctggc tgtaatcata gtacagcttt tctgatacgc tgccattctc cgtttgcagc
    20101 tgataccctg cccgaaaatc gatcaggtag cccatgatta tcgcattgtc cgactgcttg
    20161 tagatttcct tgtccggttc ataatctgtg gaaacactgg tttgagcagt gttctttccg
    20221 tacatgtcat cgtctgtctt gttgatcgca tacggcttgc cctgcaccag aatatcccat
    20281 gtattggtat tgtgataatc tgccttttcc ttgttggtca gttccgcaga ataatcccct
    20341 gtacccggaa tatcgtacat ggtcatgctg tagtctgtca caaaagtatc ttcgcccaga
    20401 accacaacat atttcccgcc gtcctttttt acggcatagg tcggttcgat ctgcggctgg
    20461 tttgctccgg tgtccgcatc gtctgcgttt ccctcggtgc tgccgcctgc attgtccccg
    20521 ttatctgtgt tgtcctgctg tttcggtgtg tactcgaaat aaatgttcca gtcatcggtt
    20581 ttgccgtaca tctctttcag ttcagacacc ggaacagaaa tggtctgtgc cgttccgaca
    20641 acgtcattgt tcgtgttctt cgcatttatg gtgaacacaa tgctgtcatc atctcggaaa
    20701 tactgataga tctctttctt gatctgaagt ccggtagtca gaaagccctt gtattcatag
    20761 tcatcatcag ggcgaatctg cggcaggctg tcagacagca cgaccttgtc cgtgaaggac
    20821 agcttattca cccagtcata ctggtcggta cgggagtacg ggctgttcat tacccaatgg
    20881 taaacatcgt tattcggtgc aaagcgaccc acaccgtccc aagtcgtgtt gacgtgagat
    20941 gtaggaatgg tgctttcttt ggtgttgccc ttccgataaa aacggatcgc gaaactgttg
    21001 tcctttccga attctgcatt gcggttttcc gctgtagcgg gggtttcacc gacacccttt
    21061 tgcacattgt cctcatcgtt gacgatatgc acacgtgtgc tggactgcaa atgctttgca
    21121 gtatagtcga atatctcata ggtccaatag tgtccacaac gataataatc atagtgactg
    21181 gtccaggcat aatacttgtc tgtgaaggac caatctgact tttccttgtc cgcaccggaa
    21241 tctgtccgca ccttacttct gccattggtg gctttgccca cagtcacatc agcatgtgct
    21301 gtagcaaccg cctcatccat gcggtagaaa cgtccggtaa cttcaaacat tccctgtgtg
    21361 gaagaatccg tctggtcata ccagatgcca tagtccggcg actttgccgt tttgccgtca
    21421 ttgttgacag tctgatagcg gttgatgtca atggtcacaa cgacctgtgt caccggattt
    21481 tccggattct ttccgttata gctctccggc ttcttataat agttggcatc gtccgcggag
    21541 aacatgtccg catcgctgcc cagcagattc agccgtgcaa agcttccgtc tgcattgctc
    21601 tcgttggctt catagagaat ttcctgtccg gaacgggtac gcttagtacc gtctgcatag
    21661 attgctgtaa ccgactgaac atagtccttg gcacgaggat caattttcag atagtacgca
    21721 tcgaatgtgt tcgacggcag gtctaatgta atatccacag ttgccgccgt attcaaagac
    21781 gcattgcgca catagtcata cgactggtgt tcctgtccgg caagggtcgg ctttgccttg
    21841 ggcagattgt tccctgtgtt cagatactgg cggaagtcca cagcgaaaga accgatggat
    21901 ttatagccga tctccaactc ggagttatcc gcactatctc cccacttctc tttttcagag
    21961 aacagataac cgtttgcgcc gtgtatatgc ttatcgcttc tccaggagca agtcgtcata
    22021 ttttctgtgg gctgggcata cttctttcgc aaatgttcca cggcactggc tgctgcggtg
    22081 tacttcttat ctccggcact ggcatcttta tagaatgcca taacagtggt gtcgaaatac
    22141 atcttggaaa catagatcgc cgtggtgtct ttcttcgtta caatatactt gtccttatcg
    22201 tatgcttctg tgatatagcc cttggattcc cgaaagccgt ccagatagtt atgtgtatct
    22261 acttccagcg tatcggtctt tccgaacagc gaatcgtcat agccgtcaaa ttcaatatac
    22321 cgcttttccg atgcggattt gtcaaccagc tttaccagtc tgccgttcag ttccacctgt
    22381 gtcaggttct cgatgccgaa caccgagaac agccgcgtgc ctgtatctct ggtaaatgtc
    22441 agtgcaccgt ctgtatatgt ataaatggta tccggtgcat cttccgtata aaaaccggta
    22501 acctttccgg cttcatccgt ttccggcagc agggttacgg ttttctcctg atcgtccgca
    22561 tctttaccag tcagaatgat attgtcaaat ttgccgaact gatcgatcag atcctgataa
    22621 attcgcagtt ccgtggtatg gaaaccagtt cgtgcttctc cctgcttttc atagggaacc
    22681 ttggcaatga tctgggtatc gtccagcacc gagatggttt cattggcaaa ttccgcacgc
    22741 agcagaaagt cacggtcata cggcacgccg aaaatggttt catttccgtc cgtgttgctc
    22801 gatgccgcac gtgtggattc tttctgatcg ttatagtaaa tatgggtgtt gacgctgagc
    22861 cccgggcggt ctacatggaa tgccacagat gcggattttg cctgattttg catcagttca
    22921 tgctgcggtg tcaccgtcat tttcgcagtc aggtcatcaa accagtcgct tgtgccgtaa
    22981 atacgcagct ccggtgcatt gccctgctcc gcagaaaggt cgcgttccag atcggcatag
    23041 gcgatttcga tatttgcaag gtatacgccc tgtgctttcc agtcagacag atctacttcc
    23101 agttcacctg cggaattttt gctcttttcc agttcttcca gggaaacaat tttcacctgt
    23161 gccgggccgg aaacaccctc cggcaggaat gctgtcagct taatgctttc gattttgccg
    23221 cttttggcat agttggagga aatcgtcacc ttgtccgaca gatagccctt tacgattttc
    23281 gcattgtcct tttccgtttt cacgcctacg ctctggagat caacagaaat cagcgacttc
    23341 cccgccttgg aatagcagcc agaagtttct ttgttctcca gcaggaaacc gtagtacaga
    23401 tcatttgcct tattcggcac tgtcaggctc ttgttgctgg tctggttcgt ttccggacga
    23461 ttggcagagg gcgtaatgac aaggttggtc tttgccacct gcatagtcgc cttcgaagac
    23521 gtcttagatg cctcgttgat gctgccaatt acctcgaatc tcgccgatgc tttcagatca
    23581 ccaacccaat ctgtcgagcc gtacagcttc agattcatct ccttgagatt gtccagatct
    23641 gtatttccgt agatgcgtga gaaaatgatc cgcacgctcc gcagacgccg aatggtatcc
    23701 ggaacggtat acgtaacatt gccgtctgca tctgcagtca gggcatcaga accgaccgtc
    23761 agactgggca caatatttgc ctcggtgtct gcggtataag ttgtattatc ccagtcgaaa
    23821 agttcaattt tttcgatggc agccgcccgt ccgtcgccga tgccgttcag gacgattttt
    23881 cgggtgtcaa agccctctat gtctttcaga cagtctgtcg gttcattgcc gacactgtcc
    23941 atattaactt gcagcagtgc atgagaactg gtagaacggg aattgttggt cagcaggaac
    24001 tgataccacg tctgttccgt ttcgctgcgg ttcggcacag ccagtgtcgg ataaacggga
    24061 tacgctattc cgtttttatc atatctaaca gactgcaccg cagaggcttc tgtgccgtcc
    24121 ttgcgaatcg catgtacgct cgctttcagt tccggcagca ccttggacac gttcagtatg
    24181 acggtatcct ccaccgtctg atccaacgcg gtttggtcat gttcgccgaa gctgtatgcc
    24241 gttttcagca cgccatgcgg agtgatgtcc ctttctgtgg taggcgttcc gtgaatctgc
    24301 acatagcagt tcccgtcatc tgctgcatcg gcaagcggaa tgctgtcgct gatacgctga
    24361 aaatacacgg tcatgccagt gagataggtc agtgtgttcc acgcagactt tgcaattacc
    24421 acattgccgt ttgcatcagg accgtaattc tccagatcgt ccagcgtaat atccgaatcg
    24481 accagtgtcc cgcgatttgt tgccttgccg tgcagtacga tcttctcgat ctttgccttc
    24541 ttcagcaggt tctcactaag caggattttc gaggcgacca gtccgccggc agtgtaatcc
    24601 tcatcgggat acagaccggt gatgtccagt gttgccggac tgatctcgga aatgctgtcg
    24661 ttggaaatca caaaacgagc cgccgcggta tcgtcattca gggaaaccgt ctgcggattg
    24721 tctttctgca ccagaccacc gatcacatct tcataaacgc ctacgccggt cagtgccggt
    24781 ttcgcctttt ctgtgcggat aacagcattt gcctgtgtga cttctttcgg ctccgaaacc
    24841 cacttgccct cgccgtcctc cgatgccgtc ggcggctggt aatagtgcac ttcgtaagag
    24901 gtgtcgatct tgttgtcata tgtccacagg tgcggaaatg caccacgcat ggtaatcaaa
    24961 ccgttgaact cggtatggcg tgggatctcc tcccgaaata caaattctac ctcgttacag
    25021 aatgtcagac cattctggtt ggtcagcacg tcgccgattt ccagtgtcca gttcttttct
    25081 tctgtcacgc cagcatcggt atcctgttcc agatgcagcc ccagttcgcc ggcagtctta
    25141 tagactacat cgccgtttgc atcggtaaag ccaaaccgga taaactggtt gatgtctgtt
    25201 ccgtctacct tgaaccagtc tttcagctga atgtccggat aatgcactgt gtcatttttc
    25261 aggcagatct gaatttcctg aagaccaaaa tcttccggca catggtctgt agaaactgcg
    25321 ttaaataccg ggatattacc gatactgtta aacccgtcca gatagtattc atctactgca
    25381 ccgataccca ctgtcttgtc tgtcacttcg ttgccgtctg cgtccttgac atacttgtgg
    25441 tgagtaaaat tctcccatgt gtgcggagtg aagttagtag aatctgaatc ctgtttcgac
    25501 cagtgaatgt tgctgccgcc gccgaatgaa attgttgtgg tcagtccgga agtgttttca
    25561 aatccggtgc tgctgttaaa gttattcgga tatgggatcg caaccatgta ttttctggca
    25621 aaataatgct tttcattagg atcactctgg ttcagttctt ctgctttctt ctcagaatac
    25681 agggtatggg acttgtccga tacttccttt tgcagaataa tcgtacctgt accggaatag
    25741 ccatatggga tagtttccgc agggatattc gaaaaatgtt ccagatccca ctgggttttc
    25801 cagtccggtg tatcggtgac atcatagacc agaatgccgc ccgcattggg ctttccgata
    25861 aactcatgat gctggtaaaa atcagccgaa aaatctttgt tctgcgtgtc cggatcttcc
    25921 ttttcccagc agagaagatc ttcgtccaga acgctccgca tcttgtccgc attgtactgt
    25981 gcctgcaatg taatcaggta gttgtcaatg gtgctgtcca gttccttaga atcattctgc
    26041 actgtgactt cgtacaccat gtagttatac ttctgccaca tgacagcatt gtttttgtct
    26101 gtactcgctt cgtccagctg cacagtagga acgctttcaa ttttcgtgct ccactccaag
    26161 ttggagttga caaatgtaac cgtacgctgg attttagtgc tggtgtccag ttcctcggtt
    26221 gaccacccgt cgccgatctg cgtattgccg gaatcataca gtctgtcata ggaaaatttc
    26281 atttccagtg ttgccgcagc attttccgga atgcttaccg cattgctcgc cgtgtaaaac
    26341 tgcatgtcaa acttcggcgt acagtttccg ctaagggaat cataggcatt tttcagagtg
    26401 atttttccgc ggacataact gttgttttcc actatcgctc cgcttgcatc atagactgtt
    26461 gtcgcattgc cgaaagtgct cctgtcgttc agctttgctt ccatgccgat atactcccct
    26521 gcctgccgtt tggcagaagg aatttcctca aagctgttca cctgtaccaa aacgccgttg
    26581 tcgttccaat acaaatacgg cagcgtcagt tccagattct gcccgcgaac aaagcctgtg
    26641 gaaaaatttg ggacaatatt cacagtcagc agtgtttgct gctgcttctg gatcatgtag
    26701 aagctgccga caccgtcatt ggtttccggc gtcagcgttg caccgtttga ggtaacggct
    26761 ccgaaaccaa cctgaaatgt gccggaattt cccaacgctg ctgccgccgc aaaaatcgtt
    26821 ccccaattgc acgaagtcag caccattacg gctgccagca tgccataaac gacacgcttc
    26881 catatactaa tttgtcgata catttcaatt cctcctgtca cgtgacacca ttatttttgt
    26941 gtcaatcatc tttataagcc atattcctga gatttcagca atttagttga ataaaatggc
    27001 taattttctg ctttgatttt tattttgaat tgtgcttttt gcacattttt ctgcggtttt
    27061 acgcttattt taattataac acttttatgt gaaattgtca atatctattg aagaaaaccg
    27121 cattatacca aaccggcagc tgtgctttgt gcaatatcgc cttaattttc ctgtggtagt
    27181 tgtttcaatt tgttttttct ccctttggat gctgttttga gaagccgctt tttttccttc
    27241 ctgatattac taagacaatg gagcatggca aaatgggaag gttgcagatg taaactttat
    27301 atgaattttt gaaaacagaa aaggcagcat acaaaaccgg ttatttcacc ggattgtatg
    27361 ctgcttaggg aaaatgattt taaggcaata ccgcacaaaa atttgcacgc agatggcgtg
    27421 caaagccgtc aggcgggctt tatgcagtgt ttccttaggg tgtgttgaaa cgaagtctga
    27481 cacgtgtctt ttcggcaaaa ttcacgattt tccacgtaaa aacaccgcct gaaaagcctt
    27541 gtcaaaacca ccagatttat gaaacgtttc ttctgttttt taaggaaaaa cgctattgac
    27601 agatgcagaa aaatcgcata taatgaactt aatccaggtg gaataatgtt gtttctccgg
    27661 aaatgaaaag aaaggaattt tttctgatga aaaaacaagt atataacccg tttctcccca
    27721 tttatgaatg tgttccggac ggtgaacccc atgtgttcgg tgaccgtgta tacctgtacg
    27781 gctcccacga caaagaaggc ggcgaaacct tctgcatgct ggactacacc acctactctg
    27841 ctccggtgac ggatctgtcc gactggcgtt gcgagggcac tatttaccgt gccgatcagg
    27901 atccggcata tcccacagac cggaagttca tgtacgctcc ggacgttgta cagggcaatg
    27961 acggcagatt ttacctttac tactgtatgt ccggcagagc cggcttcggc ggctacaacg
    28021 gaccgatcag cgtggctgtc agcgattctc cggcgggacc gttccggtat ctgggctttg
    28081 tgcactatcc tgacggcagc cccatgctga actacgtctg cttcgatccc ggcgttatca
    28141 acgatgacgg cacgattcgc atttactacg gcacatggtc tgatgaggct ctggacaagg
    28201 acttcccgaa ccacgaggaa gccattcaga cagtcatgca gcggtatcac aagagccgaa
    28261 acgaagtcct gcaaacagag ggcagtgtca tgggtccctg cacagtcact gtgggagatg
    28321 acatgctgac agtcaccagc gaaccgcggc acatcattcc ggcatacacc attggcacca
    28381 gcttcgagga gcacccgttc tttgaagcaa gttccattcg caagatcggc ggcaagtatt
    28441 atttcatcta ctcctcctgg cagaaccacg aactctgcta tgccaccagc gacctgccgg
    28501 acagagattt cgtctttggc ggcactatcg tttccaacgg cgatgtcggt tatcacggca
    28561 gaagcgaaaa agaccgcctg aacatgaccg gcaccactca cggcagcatc gaatgcatca
    28621 acggacagtg gtacgtattc tatcaccgcc tgactcacaa gtccgactac agccgtcagg
    28681 catgtgcaga acccattacc attctgccgg acggcagcat tccgcaggta gaaatcacct
    28741 cctgcggtct gaacggcaag ccgctgcacg gtaccggcac ctatccggca gtgatcgcct
    28801 gcaacatcac caacggacac atgccccacg gttccaacaa aatctacacc gacagtttcc
    28861 ccaacgcgac ccactgcggt gaggatcgct ttatcggcga aattcaggac ggcaccatgc
    28921 tgggatacaa gtacttcgca ttctccggtg caaaggagat cggcgtacag taccgcggtt
    28981 cctgcaacgg caagtttgtg gtttccgaca acatggacgg caaaaacatc gtggcagaga
    29041 ttccggttgc tccggctgat gcatggacgg aaagccgtgc tcccctgcac atttccgacg
    29101 gcactcaccc gttgtatctg ttctactgcg gcgacggtga ggcagcagtc aaggaattgt
    29161 atctggcata acaggaggga atatatatga tcgaaaaaaa catacaggaa ctgattcagt
    29221 atggtatcga aaaagaactg atcacgtccg aagatgagat cttcctgcgg aatcagctta
    29281 tggatctctt gcagctttct gcgtgggaaa cgcctgccct gccggaggtg ctgcccacca
    29341 ttgacgaaat tctggacaaa ctggtgtcct atgctgcaga aaaagacatc atcaccgact
    29401 ccaacgccgc cagagatctg tttgacaccc gcatcatggg gctgctgaca gaacgcccgc
    29461 acacagtgaa ccgcagcttt tttgccgcat atcaccgcag tccggaagaa gccacagact
    29521 ggtactatca gtacagcaag gacaccaact acgtccgtgc cggcagaatt gccaaggatc
    29581 tgcgttggac atatgactgt gaatacggca cactggacgt aaccattaac cgttccaagc
    29641 cggaaaaaga cccacgcgac attgccgcag ccaagaacca accccagacc aaatatccgg
    29701 catgccagct ttgcattgaa aacaccggtt ttgccggcac gctgacacac cctgcacggc
    29761 agaatctccg gccgattccc atttccattc acggcggaaa ctgggctttc cagtactccc
    29821 cctatggcta ttataatgaa cactgcatcg tgttcaatca gaaacatgtt cccatggtga
    29881 tcaatgcggc agtttttgaa aagctgttcg atgtgctgga catgctcccc cactatttca
    29941 tcggctccaa cgccgatctg ccgattgtgg gcggttctat cctgagccac gaacattttc
    30001 agggcggaca ctacactttc gccatggcaa aggctccggt ggaaacaccg ttcctgctgc
    30061 cgaatcagcc ggaagtacag gcggggatcg tcaagtggcc tatgtcggtc attcgcctgc
    30121 aaagtgaaaa tcgctctgcc cttgcgacag cctgcgacca cgtgctcacc cagtggcgaa
    30181 cctataccga tgccgaagca gaaatctatg cggaaacaga cggcacacca cacaacacca
    30241 ttacccccat tgccagaatg cgtggcggac tgtacgaatg cgatctggtg ctgcggaaca
    30301 accgcaccac ggcggaacgc ccgctgggac tgttccaccc gaacccgtcc ctgcaccata
    30361 tcaaaaagga aaacatcggt ctgatcgaag tcatgggact ggctgtcctg ccggcacgtc
    30421 tggcgggaga actgccggta ctggcaaggg cgttgtattc tgatgccgac ctgacacaga
    30481 ctgactctct ccgcagtcac atgccatggc tggagaaagt ccgcacacgc caccccgaag
    30541 ccaatgcgga aaatgcggaa acgatcattc agcaggaaat cggggcagtc tttgaacagg
    30601 ttctgctgga cgcaggcgtt ttcaaacgca ccgatgccgg aaaggcacag ttcctgcggt
    30661 tcgtagaaca ggttcgctcc acagcagatt aaggaaacac tgcataaagc tttgcataca
    30721 caacagaaca gccctgcacg gtctttgcaa aacgcagacc atgcagggct gtttccatta
    30781 aggcaatacc gcacaaagat tgtgcgacag atgcgtcgtc aaatttttcg t
//
LOCUS       DAWBGR010000007        29398 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_614_length_29398_cov_3.508298, whole genome shotgun sequence.
ACCESSION   DAWBGR010000007 DAWBGR010000000
VERSION     DAWBGR010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 29398)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29398)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29398
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_614_length_29398_cov_3.508298"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         8627 a         5641 c         7313 g         7817 t
ORIGIN      
        1 tttggcagag tcaggacagt gataaaaaac ggcgaaccgt ggtttgttgg aaaggacgtt
       61 gcagttattt tagggtactt tgatgtcaac aaagctgttg caatgcatgt tgataatgaa
      121 gataaaaaac tcaacgacaa aacgtcgtcg agttttggtc agcgtggcgc aacgctaatt
      181 aatgaaagcg gtttgtacag ccttattcta tcaagcaaaa tgccgagagc caaagagttt
      241 aagcgatggg ttaccgcaaa tattctgccc actttaagac gcaccggcgg ctacgttgcc
      301 aacgaggata tgttcatcga aaactatctc ccttttttgg acgagccgta tcaaaatctg
      361 ttccgtttgc agatgatggc gattaacaag ctaaacgaac gaatctgcca cgaccagaca
      421 ttagtggagt ttgccaatca ggtgtcaaac acagaaaatc tgattgacat gaacgccatg
      481 gcaaagcttg cggtggaaga agatatcccc atcggcagga atcggctgtt ccgttggctg
      541 cgggaaaacg agatattgat gtcaggaaat ttgccgtatc agaagtacat cgaccgaggg
      601 tattttgcgg tgaaggagtc cgtttttgaa acggattcta tgtgcagaac ttatcagcag
      661 acgtttgtta ctggcaaggg gcagcaatac atcattggca gactgaaaaa agaattctgc
      721 aatgaaattt aactgttcag gggggtgaaa ttatgcttgg atttatcaca ggactgcttt
      781 tgggcggaac attgggtgtt gcagtaatgg cgctgtgcaa tgccgccaaa agtgcagatg
      841 aggaaatgga aacgaaaata aagtaaggta aggtcgtttt gccagtgacg gaagctattc
      901 tgtgatttgc aaagcgactt tctttctgtc attggcaaac ggcagaaagg cgttacatga
      961 atagctttat gtcatgggta ggcggcaaaa aggcactcag ggatgccgta cttgcaagat
     1021 ttccgccata ttatgaacgg tatatcgagg ttttcggcgg tgcaggctgg gtgttgtttc
     1081 acaagccgtc cgacaatgat tttgaaatat tcaacgattt caacagcaat ctgatcaact
     1141 tatatcgttg tgtcagggac aagccgaaca agctgaaata caagctgcaa tacgtgctga
     1201 attcccgtga ggattttaac tggattgtaa gcttacacaa gcgaggatta tttccgaaat
     1261 tttgtgatat tgaccgtgca gcgaagtttt accagctgat tcggtacagc tacgccgcca
     1321 gtctggacag ttttgcttgt caaccgcatt ccatgtggag tgattttccg caaattgata
     1381 tggcggcaag gcggctgcaa aaggttgtca ttgaaaatcg tgattttgaa aagctcatcc
     1441 ggcagtatga ccgcccggtg agctttttct attgcgatcc gccgtatttt gcgacggaga
     1501 attattacaa ggatgtggga tttacaaagg cagatcatat tcggctgagg gatacattac
     1561 tacagatttc aggaaaattt ttagtttcct acaacgactg cccggaaatc agggatctct
     1621 ggaacagacc gggcattgcc atagaggaaa tcagcagact gaacaatctg gtgcagcgat
     1681 atgagggcgg ctgcatgtac aatgaactgt tgatttccaa ttacgatacc agcgagagag
     1741 caaaaatgat aaggcaactc tctctttttt gacgatgatg gaggaattga tgtatgaaaa
     1801 aagtgatttt ttcgcagatt ttagccggaa acggtgtgac aaatttcagc gggctgtttg
     1861 acgaaaatgg cctgccggtg cgtgtggaac tggagaaaaa tgcggacggc gtcgtccttg
     1921 ccgttctgcg cagagaatta tcggaggacg aacagcagaa aatctgcatg gattatgaac
     1981 actgccagga ttgtccgctg tgggaacaat gcgatgcgga gtgtgatgaa atatgatcct
     2041 tgattggttc tgtacaaaaa ttcgttcgcc gactttttcg gtaaggttca gcaacaaaaa
     2101 aacgcattac agcacgtatt ttcgtgcttt tttgaaagga agtttgtata tgttcaaaaa
     2161 caaaatattg aaaaaactgg gcgcaggtct gatggcagga ctctgcgctt tttctatgct
     2221 gggaacaaat ttcaacagtt cgtttggtgc gagtgcggca agcagttcca ctgaaaatgt
     2281 tgcatttccg tccgccgata cggttatcgc aaaagcggca accctgctgg gaacgcctta
     2341 caccttcggg aacaaaggtt attctggtat ctacacccag ggcgcatatg cgccgctgtc
     2401 cgtcagcacg attaacagtc ttggtatcga ctgttccgga cttgtgtact acacgctgac
     2461 ccagctgggg tacagtacat cgggattttc gtggaacaat cccgttcctg tggacacagc
     2521 acattggctg acggtcagtg acagctgtac gattacctac ggcggaaaga cttccaacat
     2581 tgacattgaa aaacagaacg tcaaaacgga ggagcacccc tactgggaat gcgccgacgg
     2641 ctccaccatc acggctggtt cggtggttgt ggcggagaca aaaaacatca accatgcttg
     2701 gatttacatg ggcgaattca attcccgaaa cgatgtggta tcctatctga aaagcatcgg
     2761 cgttccggaa agtctgatca cttcatcaac agttggtagt ggaaatggca atggcggtac
     2821 ccactggcgc atcgaatcca atggttccca gggcgttgtc atcaacaaca atacggacgg
     2881 aaaaagtgca accgcgctga atacttcggc ttttcgtatc acgcagaatg atgcaacctt
     2941 ccgtatcaaa aagtgtctcg atggaacatc ccaaattgtt ggtaaatcga cggttgacgg
     3001 cagcacggcg aagtacggcg tttacgccga ttctgcatgc aaaactaaag tcggagaaat
     3061 tacgattggc gcagatggta caggttcgat aaagctgcct atggggagtt attacgtcaa
     3121 ggaaatttct gctccgaagg gttatgcgct gtcaacaaaa gtttacgaac tgaaagccaa
     3181 tcagaccgtg accgtgtacg aggactatga aactggctct atcaaaatca acaagacctc
     3241 tgaggataat gttgtgaaag atatcgaatt caaggtgacc ggttcggacg gcagcagtta
     3301 cacgaaaaaa acaaattcca gcggcgttgc ggaattttct ggactgaaag tgtacaaccc
     3361 cagtacaggc aaggcgatca cctacactgt caccgaaatc aatgttgcca caagatatga
     3421 aacgccaaag gcacagaacg tgacgctggc aaacggcaat gtggatttaa cagttaacct
     3481 gacttttatc aatgacctgg tgaagggcaa tctcaaaatt aacaagcaat cggaggacgg
     3541 cgaaaacggc gaccgtgaat ttacgatcgc aggcggcggc aaaacttata ccataaaaac
     3601 aggctctgac ggcattgcca tactttctga cattcccgtc tatgacagta ataacgaaaa
     3661 gattgtctac accatttctg agaagaatgt gccgataaaa tacgttgttc ctgctgatca
     3721 gacggctacg ctgacggttg acgataccac aacgaaaacg ttccaaaatg tgctgaaaaa
     3781 atttactgcc gaggttgtca aaaaggatgc agaaaccggt tccgcacagg gtgacgccac
     3841 acttgcagga gcagtttacg gattgtatct ggatggcgat cttgtggaca cctataccac
     3901 tgattcggaa ggatatttca agacaaagga atatgtttgc ggcaattata cgcttcagga
     3961 aatctcgccg tccaaaggat atctgctgga tgagacggtg cattccgtcg gcgcagaagc
     4021 caagaattat acggtggaaa gcaatctgat ttccgttgcc gtgaccgagg atgtggtaaa
     4081 gggaaacatc tccattttga agcattctga tgatggcagc acgcagatcg aaacatcgga
     4141 agtcggcgct gaatttgagg tttatctcaa gagttccggc agttatgaaa ctgcaaagga
     4201 ttccgaaaaa gactacctta tctgcgatga aaatggattt gcaaagacaa aggatttgcc
     4261 atacggcgtg tacacggtgc atcagacttt cggctgggaa ggtacggagt tgattgcgga
     4321 tttcgatgtg tacgtcagtg aaaacggcaa aacctatgcc ttccttatga acaacagaga
     4381 atttgaaagc tatgtaaaaa tcgtgaaagt ggattccgaa actggaaata ccattcccta
     4441 cgagggcgca ggatttgaga tttacgattc taacggcgaa aagatttcca tgtcctacag
     4501 atatccaacg ccaaccacaa tagacgtatt ctatactaat tccgaaggct atctgatcac
     4561 accggaagtt ctttcttacg gagaatacac gcttgtggag gttcaggctc cctacgggta
     4621 tgtgctggac agcactcctg tgtccttcac aatctccgct gaaaatgcag aggaagaaaa
     4681 cgctttgaca atcgtgaaag tcaacaagga aaacatcgct cagaaaggca gaatcagcgt
     4741 tcagaaaacc ggcgacagct tcgtgtctgt tgcagcggcg tcctccgctt acacagatga
     4801 aaacggcgag atgatagtaa atccgaccat ctacactccc gtgtttgcga acggaaatct
     4861 ttccggtgca gtatttcagg tgatcgcctc tgaggatatt gttactcttg acggtacaat
     4921 tcgtgcaaat gcaggcgacg ttgttgccga acttaaaacc gatgaaaacg gctatgcgga
     4981 aacagatttg ctctacctcg gaaagtatga ggtcaaggag attactgtgc cttacggata
     5041 tgttttgaac agtgaatcgc aggttgtgga gcttgtatac gcagggcagg aaatcgctgt
     5101 tctggatacc gtaaatacgt catttgtcaa tgattatcag ggtgttgaga tctcactttc
     5161 taaggttatg gaaagtgatg aaaccttcgg catttcggca gaaaacagcg cagtgaatgt
     5221 ccgcttcggg ctgtttgcag cggaagaaat tgctgctgct gacggctctg tcattcctgc
     5281 ggacggtctg attgcagagg tttcactgga tgaaaatatg acggctgtca ttacagagaa
     5341 aatcccgttt ggcaagtatt atgtgcagga aattgccaca gacgagcatt acgttttgaa
     5401 cggcgaaaag tatcttgtgg actttgaata tatgggacag gaagtcacaa cagtcaatat
     5461 tgattgcggt cagtttgaaa atgagctgaa gcgtggaaaa atcgaaggaa tcaaagtgaa
     5521 tgaatccgat gaaccgctgg aaaatgcact gtttggattg ttccaaacag actgtacaga
     5581 atttgtggaa agcaatgcaa ttatgacggc aatctccgac gaaaccggat attttgcatt
     5641 tgatgaagtt ccctacggcg agtatattgt caaagaaatt gaggctccca caggctacat
     5701 tctcagtgag gaaggctatc tcatgacaat ttctgaggac ggtgaaattg tgggaatcac
     5761 agctgaaaac aagccgatta ccattgaaat ctccaaacag gatgtgtatg gagaagaact
     5821 ttccggcgca gaaatggagc ttatcaacag cgatgaagaa acggttgaaa aatggacttc
     5881 cgacggcaaa aaccatgttg ctacggagct tcctgccgga gattatacgc tgaaagaaat
     5941 cgctgcaccg gacggttatg tgattgcaac tgatatcagt tttacagtcg acgtttacgg
     6001 caatgttact gtagaaaacg tagacgcaat cgctgtttcg gaagatggta atcccctgat
     6061 tgttatggtt gacgacacca caaaagtgcg gatttccaag caggatatca ccactggtga
     6121 ggagcttcca ggcgcaaagc ttcaggttat cgacaagaat ggacttcaac caaagaaccg
     6181 cattatttgg aggcggttct caaagctggt gaaacctacg cattgcatga agtcattgca
     6241 ccggacggct acgctattgc aaatgatgtg gaattcacgg tgaatgcgga tggcacagtg
     6301 acggaagttg tcatgtacga tgatgcggtt ccggtgacaa aatcgacgcc caataccggc
     6361 gacagcggtc ggaattcgct ggcatatctg atgcttgccg gaagcgtcat cattttcgca
     6421 gcacttgcca tcagcagaaa aaagaagcgc agaagtgaaa aggctgataa aaacgtcgct
     6481 ttctgcctgg attttgttga ggtggaacat gaaaacgaat aagaaaaaag caatccgcat
     6541 ggggctggct gtgaccgcag ctgctctctt ctggggtgga atgtatttgc tgtttggaag
     6601 cgatgctgcg cagaaaatgg agtggaatca ggtcaaggat tatgcgccgg attctgaggt
     6661 atgcgagacg ttggacgaag ctgtggctga aaccgggaac atacctgttg ttgctacagc
     6721 aaccacgcac atgctgacag cgcaaattca gcaaatgaat gcaaattatg ctaacgccat
     6781 cggctggatt tatgtgccgg atacgcacat caattacccc atcatgcagt cggaggataa
     6841 cgacttttat ttgcatcatg gggcagacgg cggttatttg tatgctggtt ccatcttttt
     6901 ggattaccgc tgtaacgctg atttttcggg cgtttccaat attttatacg gtcacaatat
     6961 gagcaaccgt tccatgtttg cggacgtgat aaatttcacc gacagaacat attttgacag
     7021 tcaccgctat ggttggctga ccacggaaaa tgatgtatat tgcgtgtgtt ttttcgcagt
     7081 ggatgtaacg gaaaacacag gcgaaattta caatacggat tccagctggc ggactgtcat
     7141 gagtgaggcg gtaaatcgtg cggtaattta cgatgacatt gaaattgccg atacggatcg
     7201 tcttgtgatg ctgtccactt gcactggcgc aaacagttct tcccggacag ttttggttgg
     7261 aaaaatcatg gaggatgaga atatatgaaa ttgagtaaaa gaacgaaaaa gatcatgatt
     7321 gcggcggttt ctgttattgt gctggctttt ctttgcagcc tgacggcgtt tgcagatggt
     7381 gaggttgccg gagcagtgga aagcacttgg aatgctgcca agggacagat caagaccatt
     7441 gtcaacaatg tggtgttccc ggtggttgat gtcattctgg cgattttgct gtttgtgaag
     7501 atcgcaactt catacatgga ttatcgcaaa catggacaat ttgaatggac ggctccggcg
     7561 attttgtttg gttgcctgag cttttctttg actgcaccgc tgtatttatg gagtattgtg
     7621 ggactctaag gaaaggatgc aaatatgccg tatattccat acacagagga ggaaaagcag
     7681 cttgcgaaca gcgtggactt gacagaattt ctccgcatga ggggcgagaa attagagaaa
     7741 gttgggcgag aatacaagct gatttattat gacggcagtg ggaaacatga cagcattatg
     7801 attcacggcg cagcttggtt cgatcacaag aatcaggtcg gcggcggtgc catccaattc
     7861 atgcgatatt tctatggcat ggattttcag actgccatga acgagttgct tggaagaagt
     7921 gttgctccca ttgcccacag tccgccgaaa gcggctgtgc aggagaaaaa gataaagaaa
     7981 ttccacttgc cgaaagccaa tgaaaatatg catcgtgtgt atgcttacct cataaagcag
     8041 cgctttattt cgccggaaat tatcacacat tttgcaaaat agcacacgct gtacgaggat
     8101 gcagaacatc ataacgcagt gtttgtgggc atggatgaaa acggcgctcc ccggcaggcg
     8161 catctgcgct ctaccaattc ctttgggaaa gcttttcgaa ttacttgtga gggttccgac
     8221 accagataca gctttgccca ctttggtgag tcaggcaaac tgtttgtgtt tgaggcgcct
     8281 atagatatgc ttagttacct gacattgcat tcggaaaact ggcagaagca tagctacatt
     8341 gccatgaacg gtgtgtacaa aaatgcggtt ttcacagcgt tgaaaaacca ttgcaatctc
     8401 agtgaaattg tactttgcac agataacgat gcaggaggaa ttgatgctgc tgaacgctta
     8461 acagatattc ttcacgagaa cggatatgaa agcgtatcga gaattattcc gaagaataag
     8521 gattttaatg aggatttgaa gcaacttcac ggcgtggagt tcctatctgc tgttcctcat
     8581 ttccgtaaag aaaaatacca ggaaattgcg ttagatttgc gatatttcaa atgcaatcct
     8641 gacagaatgc tcaaccggat ttccaagaca tttcaaaatg gacaataccg atatcttgct
     8701 gaatatgcct tagtagcatc ggcatttttt attagcaaaa gcgctgaaaa tacgatgttt
     8761 gaaaagttga aaatcaagct gtcggacgag tacagagcgt atgcagacaa gggaaaactg
     8821 tgtgcaaaac aggacaactt aaaatccgct tatcagtcgg tcatgcgtga tttgcggtat
     8881 acgcagtcac gtaccaaaga acagatgatt cagacggcaa agcagctgta tcagcttgca
     8941 gattgtgctg tgaaatgtga ggtagaacag gaattgtctg caccgcagct catgcaggaa
     9001 gaaacgcaga ccgaagagcc atcggaggtg ctgcacatgg aatatggata acacggaggt
     9061 gttctaaatg acacaaagtc aactgatcgg cattattgca atgtcggtca tttttgtttt
     9121 attactaata ctggcatact gttccaataa ctattccctc aatggaatca agcggaaaac
     9181 tgttgggcaa ggtcagcatg gtactgccag atttgctacg aaatcagagg tgaaacacac
     9241 gtatacccaa gtcccatttg atgtggaaaa ctggcgaaaa ggcagatatc tgccaacagt
     9301 tcagggaaca gtagtcggct gtaaatctca tggaaaacag acgattgctc ttgttgacga
     9361 gggcgatgtg cataccctta tgattggcgc tgctggcgtg ggaaagaccg cttttttctt
     9421 gtatcccaat ctggaatttg cttgcgccag tggtatgtct tttttgcaaa ctgacacgaa
     9481 gggcgatgtt tttcggaact gcggcagcat agcgaaaaag tattacgact acaatgtttc
     9541 tgtgttggat ttacgcaatc ccacacgctc cgatgaaaac aatatcctgc atctggtgaa
     9601 caaatatatg gacatctatc tgtccgataa gcacaacctt tccgcaaagg caaaagccga
     9661 aaagtacgcc aaaatcacgg cgaaaaccat tatcagtatc ggcggagggg acgcagcagc
     9721 ttacggtgca aacgcctttt tctacgacgc agcggagggt ctgcttgctt cattaattct
     9781 gcttcttgcc gagttcggtg agaaaaacga acgccacatt gtgtcggttt tcaagatgat
     9841 tcaggacttg ctggaacctg tgtgggaaaa gcaatccatc aaaaaaactg cacccaaaag
     9901 cggcttcaag gctttgatgg aacggctgcc ggatgagcat aaggcgaagt ggcttgcagg
     9961 ttccgcattg cactctgcag atcaagcgat gttgtcggtc atgagtacag ccctgtcccg
    10021 tctgaacagc tttatcgact cggaaatgga gcagattctg tgttttggta ctgcattgga
    10081 tgcggaaaaa ttctgcaagg aaaagtcggc aatctttatc gtgcttccag aggaagatgt
    10141 cagcaaatat ttcatggtat cgctgattat tcagcaacta tacagagaaa ttcttgtgat
    10201 tgccgatgag aacggcggcc gcttgccgaa cagggtaatg ttttattgtg atgaattcgg
    10261 cacttttcca aaaatcgagg gcgcagaagc catgttttcc gcaagccgtt ccagaagaat
    10321 cagcattgta gcgattattc agtcctttgc gcagctgaac aaaaactacg gcaaagaggg
    10381 gcaggaaatc atcacggata acacacagct aaccattttc ggtggatttg cgccaaacag
    10441 tcagagtgca gaagtgttat cgaaagcgct gggagagcag acagttttgt caggcagtgt
    10501 atctcatggt aaagaaaaat cccaatcgct ccagatgatc ggcagaccgc ttctcacagt
    10561 ggatgagctg aaatctatgc caaagggaca gtttattgtc atgaaaacgg gaacgcatcc
    10621 catgatttcg ccgctgaagc tgtactttaa gtggggcatc tactttgaag aaccgtatat
    10681 tttggaggac aggggtgcaa gaactgtcac ctacatgagc aaggaagcac tgatgcgtga
    10741 agtggagatc cagtatccgc agatgaaaaa aacagtttcg gaagaagatg aagaattgga
    10801 agaagaaacg ccaaaacgaa gaaaaattcc gaggacagga ggtgtgtaaa acctgaaatt
    10861 tgcaagagaa atttatgagt atggactgcc tcacagggca gttgcggtgt acatgtatct
    10921 gtccaatcgt gcaaacaaaa acagggagtg ctatccgtcc gtcaggacga ttgcagaaga
    10981 tctgcatttg tcgaaatcca cggtgtttcg tgcattaaat gatctggaaa ctgcgggact
    11041 cattactcgt gaaaagcggt ggcgaacaag cggcggacgc agttctacgt tatatcaatt
    11101 aaaggagtgt tgaaatgcat acagaaaaaa tgtgcagctg ttatcgtatt acaaagcggt
    11161 attttctgaa aaccaaacgg aaatgccgct gtttttattg ccgttccaac cggcagtatg
    11221 cgaacatgaa gcggctgtta tcggcggaag aaaaactaag agaatggagt gtgggcgatg
    11281 tttgattgga tcggcgatgc actgtcgtgg gtgggcgata agttttccga tgtagggact
    11341 gccatttctg aagccgtctg ggacgtgatg ctggagtggc tgttcaacac catatacggc
    11401 gcagttgctg agttttttgg tatgatgaat ggcatgggtg cggatatttt cagccagtcc
    11461 tgggtgagcg cttttttgca gctgttttcc ctgtttggtt ggacgctgtt tgccgcaggg
    11521 ctggtagttg cagtatttga tacagcaatc gaatatcaga ccggacgtgc aaacattcag
    11581 agtacctgtc tgaacgtgtt gaaaggcttt atggcggtca gcttgttcac gaaagttcca
    11641 gtggagatgt acaagttttg catcacgctg caaactacat tttcaggaga tctaatcggt
    11701 tcatttatcg gtgcaactcg cggagattta ggcacaacgg cagaagtggt gctaaaaaat
    11761 atgggcggat attcagggtt attaggactg ctgaccatga ttgcgctggc ctactgtgtg
    11821 gtaaagctat tctttgccaa catcaagcgg ggcggtattt tgctgtgcca gattgcggtg
    11881 ggaagtctgt atatgttctc ccttccaaga ggattttccg acggtttcaa cggctggtgt
    11941 aagcagatct ttgccctatg tctgacggca tttttgcaga caagcctgat gttcttaggg
    12001 ctgctgacat ggcagaccaa tatgctgctg ggacttggca tcatgctggc agcaaatgaa
    12061 gtgccaagaa ttgcgcagca gtttgggttg gatacttctg tgagagtcaa catgatgagc
    12121 gtaacccata cggtaaattc tgcgattcat gtaggaaaat ttgtggcaag aaaattggca
    12181 tagggggtgc attatgaaaa catatattta tccgcaaaac ttgaaagcga cctccagact
    12241 gtggctctgg agtattcggg actttctgat gatttgtctc ggactgattc tgtcagtagt
    12301 aattttaacg cagctatgga cgtttctgcc ctttgccatt acggcagtgt ttgcatttct
    12361 gagcattcga ttggaggaaa cgacggtaat cgattacatg gtctgtgcgt ttcggttttt
    12421 cgtgaccgca cagcagttat tttattggaa gtgaggatga ttatgaagaa taaaaaatca
    12481 acgcagaacc tgtttggact gcggacattc ggtaaataca gtttgcagac ggacaaggct
    12541 gaacttgctt ttttcagtgt gcagcccacc aatatttctg tgctgtcggc tgagaatatt
    12601 gatgtgaaaa tctatcatct gatgatgctg ctcagcatga tcccggaact ggaaatcatt
    12661 tgtcttgaca gttgtgaatg ttttgacggg aacaaggtgc atatcaagaa ccgcttgaaa
    12721 tctgaggaaa atccaaatgt gagaaagctg ttgaaacagg atgcggcatt tctggacgat
    12781 attcaggttg agatgtctac ggcaaggcag tttctgtttt gcattcgttt tagggacaag
    12841 aaggaggaac agatatttca gcagatcaac cgcacagcca aagtaatttc ggagcatgga
    12901 tttgacgtgc ggcgcatggg aaaatctgac gtcaagcgga tgctggcgat ttatttggaa
    12961 gcgtccatgc acggagagga aatttccgat attgagggag aaaattactt agagacagcg
    13021 taaaaacgga ggtattgaaa ttgaaaatgt ttcagaaaaa gaagaaaatg ctcacgcaag
    13081 aacaggcgga agaaattcgg attcaggaat tttttgaccg tatcgtcccc agtacggtca
    13141 gatttttcac cgatcatttt atttgtggaa acagctgtaa atgtgtgtgg gcagtgcggg
    13201 aatggccaac cacaaccgaa gagcaagcga ttctggcaca ccttgcagac aggaattctg
    13261 tgacgctccg catttacaac agacttgtgg acagtgcgga gcagcggcag atcattcagc
    13321 acgccacgcg acggaacaaa ctgatgtcca ccaccaacga cgtgacggaa tccctgacag
    13381 cggagagcaa tttgcaggat gtggtgcagc tgattgctga cctgagacga aacaaagaac
    13441 cgctgctgca ctgtgctgtc tttatcgaat taaaggcgaa ttctgaggac aagctgaaag
    13501 aactgcaatc ggatatttta atggaattga cacgttccaa aattacagta gacaggttga
    13561 ttctgcgtca aaaagagggc atgctttcgg ctttgccctt tggatacaat gcgtttggaa
    13621 gcctgtatga acgtgtgctt ccggcgagtt ctgtggcaaa tctctatccg ctgaattatt
    13681 ccggcaaaac ggatgaaaaa ggtttttata tcggtcggga taagtttggc acgaacattc
    13741 tggtagattt tgacaagcgc agtgatgata aaaccaacgc aaacgtgctg attctgggca
    13801 acagcggtca ggggaaatct tatcttatga aactgttgtt gacaaatctg cgtgaatccg
    13861 ggaaaagtgt gattattctg gatgcagaac aggaatatgc tgatctgacg aacaatcttg
    13921 gcggcactta tctggatttc atgtcgggag aatatatgat caacccactg gaaccaaaaa
    13981 gttttggcga agcagagcgt gatgaaactt caccggagcc gttccggaaa gtgacaaggc
    14041 ttagtcagca tatctcttat ctcaaggact ttttccgagc ttacaagggc ttttccgaca
    14101 gtgagatcga caccattgaa attatgctga tgaagctgta tgcgattttt gacattgacg
    14161 atctgacgga ttttaacaag ctgaaaccaa cggattatcc ggtgatgcag gatttatatg
    14221 atctcattga aaaagagttt atgatctttg atcatgagaa aaagcactta tacacagagg
    14281 aaactttgca gaatatttgc ctgggactgc actctatgtg taaaggtgcg gagtcgaaat
    14341 actttaatgg gcatacgaac attaaggatt cgcagtttct gtgcttcggc gtgaaaggtt
    14401 tgatggatac caacaaacgg ctgaaagata cgctgttgtt taacattctc agctatatga
    14461 gcaatcagct tttgggggtt ggaaataccg ttgcagcaat tgacgaattg tatttgttcc
    14521 tcacaaattt aacagccatc gagtacatcc gtaatgccat gaagcgtgtg cgcaaaaagg
    14581 agtcggcgat ggtgatcgca agtcagaatg tggatgattt tttgattccg gaggtacggg
    14641 agttgaccaa accgctgttt gcgattccaa cccatcagtt cttgttcaat gctggaaaca
    14701 tcgagccaaa ggcgtatatc gacaccttgc aattggaaat gtcggagttt tcgctgattc
    14761 gatatccgga gagagggcct tgtttgtatc ggtgtggaaa tgagcggtat ttgcttcagg
    14821 tacatgcgcc ggtttacaag gaaaagctgt ttggaaaggc agggggaaga tgagcgtcac
    14881 agtcgctgct gctgccaaaa aaattgctgt ctaccttgcc acggataagc ggacatggaa
    14941 agtggttggt acgattatcg gtattgcaat cgccattgtg ttactgcctg tcatgctttt
    15001 gctggcaatg ggaaatcagt tgtcatctgc ggaaacccag agcatcgatt attctgacta
    15061 cgtgcagaat ctctctgccc agcagcagtc gcagctttct cagatggaat cggacggaac
    15121 agctattgcg gatgcgctga cgaaactgaa cctaaaaaat caaatcgtga aagcacagat
    15181 gctctatctc acctattttg acagtgttca aaagggtgag aatttttttg cagaatacgc
    15241 cgattgtttc aaggaaagcg atgataaaaa gctgattgac agcttaaatc agaagtacgg
    15301 actgggcatt gattatacgg aatttatgcg ttcctatgct gtaatttcta atgtcagcat
    15361 tgatgaaaat tggttcttaa atttggatgt gaagaacaac attgatctgg cacgctgggc
    15421 agaaaatgcc tatgaaaccc agtggggata tgttccacac agcgatggga atgtgctgta
    15481 tgcagattta tatcaatctt tgcaggagca atatcccgat gagattacag aagaatgtga
    15541 caagtggcgg agccgcaggg ctgttgacaa ttacaatctg ctgcggtcgt atctctggta
    15601 cgatgcagaa agccgagaga ttacagtaga ggggtacagt gaaacggtgc aggaactgta
    15661 tcaatctgca tctgtaaaag gcagtatgga cacgcttccc aaaacggtcg gaacggtggt
    15721 gatctgcgac aatatcatcg gcatttttgt cggtgatagc tatgttgtgt acgccaaatc
    15781 cattgcggac ggtgtggtaa aagagcctat ttcggagggg aactggatgg cttggtttga
    15841 aattccttgg attcagtacg gcgaggaaaa gagttttagc aatgacattc agtttgagga
    15901 atatgaccct gctgtgaaaa acaatttgga cttggtgcag tgggcaattc aggcgcatga
    15961 aaacggatgg ggttatgtct acggcactta cggaaacgtg ctgacggaat cattgctgca
    16021 ggacagagcg tctgtgttcg gcggtcaggt cacaagctac atggatttca ttcgttccaa
    16081 ttggatgggg aaacgcactg ctgattgtgt gggtctgatc aaaggatacg gttggtataa
    16141 cgcagaatcc ggcgaaattg tggtaggttc caacggtatg atggacgtga ctgcaaatgg
    16201 tatgtttgaa gcggcgactg tcaaaggcac gattgatacc attccggaag tgcccggact
    16261 tgcggtatgg catcagggac atatcggcat ttatattgga aacggcgagg taatcgaagc
    16321 gatgaatacg atgcggggcg tgaccagaac gaagcttgtc ggacggtctt ggacgcactg
    16381 gctacagatt ccgtacatca gttatgtgga ggaaaaggaa aatgagagta cacttgaaaa
    16441 ataatgggta tgcgttatca gcagagttct cgatgaccgt ttttgaaatg caggatgcac
    16501 tggacaagct acaaattcca ccggaaacca gagctgtcac gtttcagatt tatgattttc
    16561 ggaacatgaa tctgtcgtcg gagctgtgtg aaaaggagtt tacggcggat attttcaggc
    16621 tgaatctgtt cgttgagcga ttggaaaatc tggatgatac agagatggca gcgatgaaaa
    16681 gtctgctgca aacttatccg gaaagcgatt ttgaggacat gcttttgatg acctacgggt
    16741 tggattctgt aatggtttat ccctgtgagg actgtcgtga actgggcgaa acggtcattg
    16801 aaaacgaatt gctgccggaa ttggagggct gttccgatga gattctggaa ttgcttgacc
    16861 gtgaaaaagt cggtcagctc atgcaggaac gtgagggcgg tgtgtttgtg gatggatatt
    16921 attgtgtgac atcggattac gaaccgcccg acattcattt tgaatttgga gaaccagagc
    16981 aatgtttttt tcgtctgctg attgcaccgg atacacagaa tacggcgcag gcagggtgga
    17041 tttccctgcc ctgtgatgtg gaaatattgc ttgaagttta tgatcagact tgctgtgaat
    17101 tgcagtcatc actgccgtgg attacgtcag acagtctgaa aaatatgcat cagatcggca
    17161 tactgaatgc cttggcagaa cggatgtcgg aactttctca caatgatttt gtgaagctga
    17221 aagcggttat ggacagcaaa cggattcgtg agattccaga tgcgctggcg tgtatggagc
    17281 atttgcctga gtatgaatgg gatcggtctg ttcgggattg cagtgagttt ggtcgggcgt
    17341 atctggcgaa aaatctgcct accaattttg atatttctct tctgaaaagt ggagattttt
    17401 atgattttgg cagtcagatt atgaagcaaa caggcagtgc cgtgacatct tacggtgtga
    17461 tttccggcag gggtcagagc ctgtattctg tgctgacaat gcagccggag cagcaacttg
    17521 aggaggacat ggaagaagat tttgaaatga aaattggagg tctgagctta tgaaaaaatc
    17581 agtcacagta agcgttgatt ctgaaaaatt gtctgcgctg gagatgtatc tggggcagaa
    17641 aaatatgaag ctgtcggagg aacttgaaaa gtttgcagag cagctttatc agaaatatgt
    17701 cccctccaat gtgcgtgagt ttatcgagtt gaccgcatca aaaaagccgt cccgcaggac
    17761 aaaactgact gctcctgtgg aaagtgaaaa tcagtcggag cagtaattcg ccgcagttcg
    17821 caccgtgtgc caccgtgtga cgagattcgg cggtcggtgg aataagttta ccctaaatgt
    17881 agtcggagcg aatttggggc atttatcgca gagtttgaga atggtagaaa ttagatgatt
    17941 tcgggtgggt ttgtgggtca tttattttat tgcaggttaa agttttggcg tcaagccgcc
    18001 aaaacaatca catcggacga gcaaagctcg ccgattctgc gagagttaca aaatcttgta
    18061 ggaatggagt cagaaacgga gggtttcaaa gatcatggag tcagatttgc aaaaaaagag
    18121 aaaaagtgcc gaagattcag cactttttga taatgtcgca tcagaaaagc ttaagtttcc
    18181 tttgtacatt ttttcagaca cgatggaaaa ggtgaatatg ctgtatgaga ccgacaactg
    18241 tcgttccaaa acggagttta tggaaaaggc aattcggttt tactgtggtt atcttctgaa
    18301 caaagaaagt actgctacag agtttattgc tccgcagctt gctgtgatta cagagggtat
    18361 tgtgaagggc agcgagcaaa aactttcccg tgctcttttt aagttggcag tggaagttgg
    18421 tgcactaaca catatgcttg cggcaatcaa tgaaattgat gacgaaactt tgaaaaagct
    18481 tcgtatcatg tgtgtggatg aagtgaaacg catcaatggc attatcaatt ttgaaaaagc
    18541 ggttcgctat caaaggagcg gtgactaatg tcgttattaa ttgttacaag ccgatatctg
    18601 aaaagtggta ctgcaaaaac aaaatctcaa cggaagaatt atacgaaata tattgcgact
    18661 cgtgagtcgg ttgaagttcg tgagcagagt aaaaaatatt ctacggagaa tcagaaaaag
    18721 ctactgaatg aactgctcaa tgattttcca gaatcaaaaa aatatttgga atttgaggat
    18781 tacaaaaata atcctacagc tgaaaatgcg tctgaactga ttagcataat tattgagcgc
    18841 aatgcggatg tgattggcaa ccgtcagaat tttgtcggct acatggcgat gcgacccggc
    18901 gtggaaaagc gtggcgaaca tggacttttt aatgaaagca atgagccgat tgtgcttgat
    18961 caggttgcag aggaagttgc gaatcatccc ggaaatgtgt ggtcgcacgt tgtctcatta
    19021 cgccgtgaag atgcgattcg gctgggttat gacaactctg accgttggcg tgagcttgtc
    19081 atgcggcaca ttgctgacat tgcggagcag acgataattc cgctgtgcaa tctgaaatgg
    19141 tacggcgcat ttcacgatac cacgcatcat ccgcatatac atctgattgt ttattccaca
    19201 aattcgaagc agggattttt gacgaagcaa ggcattgata aaatacgttc tgtatttgca
    19261 aatgatattt ttcatgatga attgcagtcg atctatcagg agcagacgct tcggcgtgat
    19321 gaattgaaaa tggagtctaa aaagtatgtc gaacagttgg tgaagcagat tgcggagaat
    19381 cattttcata atgcaaacct tgaaaatctt atccttactt tgaaaaatca acttgataac
    19441 acaaaaggca aaaaagttta cggttatctc aagccggaag tcaagaaaac ggtggatgag
    19501 attttcagaa ttctgtcgca ggagaaaaat atatctgaac tgtacgagaa gtggtgtgaa
    19561 cttgaacggc agaagtacaa aacctatacg cagaaggaaa aggaattccc tttgctatgg
    19621 gagaacaagg tctttcagcc tgtgcggaac atgattattc agcaagtttt gcagatgcaa
    19681 ttccctgctg aagaaatgtc agttgtagaa ccagaactac cagagcattt tgtggaaatg
    19741 gagattctgc cgcaaattga tgaggaacct attgattcag aaaatcaata ttatctaaag
    19801 tggagcgaca actataaaac ggcttgcaaa atcatttatg acaaacagtc aaagactgag
    19861 gattttcaaa aagcggaaca gcttttgctg tcagagacta agtccggaaa tgtgcttgtg
    19921 atgtttgaat tgggaaagct gtatacatct gaaaagctgg gcaaaaagga tgtggaaaaa
    19981 tcggttgcac tttatcagga agcgttgcag ggatttttat tgattgaacc gaaatcgaaa
    20041 aagatcaagc cacttttgca gtatcagatt ggtaggatgt atatgaaagg tttgggaaca
    20101 gacgtggaca tgccgaaagc aattacatat tttcagcagt ctgcagcaca cggaaatgtt
    20161 tatgcgaaac gaattattgc actggaatat atcagcggcg agtttttgga acaggacgtt
    20221 gacaagggta ttttcgtgct gacggaatgt gcggacagcg gtgatacgct gtcttgttac
    20281 aagcttggaa aactctatct ggacggcaaa ataactacac agaatctgga taaagcggaa
    20341 aaatatctga ttgcggcgga tgagaacgag tatgcttttt acgcgctagg caagttatat
    20401 ctgacagagg aaaagcgtga tgtgcagtgt gccgtttcgt attttgaaaa gtgttcgaat
    20461 atgaatacgt gggcaagtta ctggttggga aaaatctatt tatttggctg tgaagaagtt
    20521 ccgcagaatc gggaaaaagc gttgcaattt ctcacatttt ctgctgaacg gggcaacgga
    20581 tatgcgcaaa atatacttga taatatggaa cagtatcagt ccgaaatgtt gacaaatacg
    20641 attttcagct tgtttgtaaa tctcagtagg tgtctttctg aggactacaa tcagaaattt
    20701 caatccggca gaatatccgt ggataaaaag cttcgccgaa tgatgcagga gaaaaaacag
    20761 gcacttggca tgaaggaaga acgcacgcaa acacaggagc agtcttatta agtgccatat
    20821 gccccgtgtc atgatgccat gggaatgtga agtagaaaat agaagatagt tttatagcaa
    20881 agaggaaaat atatgaggcg tactgttttt gatttcagta cgccttattt tttcgtcaat
    20941 tccgcccata catggataaa acaagaaaat acatagaaac cacgttgctt tcaaaacaaa
    21001 tactgacaag aatctgacgg caaataaatt tatgctgaca aacaaagtct gcttacataa
    21061 ctccgcttca tctgcatgga agaatgcaca taacggttca aagtaatgct ggcgtccgcg
    21121 tgccccagca gttcactgag tgtctttgga tcgaaaccgt ttgcaataca aaccgtggca
    21181 taggtatgtc gaagcaaatg aaacggtaca ttccgcagct gacaagcttt caaaaccgcc
    21241 ttaaagcgat attgcatcgt tctcggttct tcaggctttg ccgtgccaga aagcaaaaac
    21301 agcttgctat cttttttctg ctgtgataat aaatccagca aaaatgccgg aatcggtaca
    21361 gttctaattg atgttatgct ctttggagaa ccgataatca cctcggaact gccacgcttg
    21421 ttgattcgct gaacagttct tttcacggaa agcgttccgt tttcaaaatc aatatcgccc
    21481 caagtcaggc cgcatagttc tcccacacgc aaacctgtga acagacacag cagaatccct
    21541 aagtttgttc tggtaaggtt gtgcaaaaga tattgttcca gctgtcttcg ctctgccgca
    21601 ttcagcacat ccaattgttt cttctccgcc ttgggcatag tgaaatttgt agattttacg
    21661 ttgtattcct gcactgcata ctgctcgata ctgcgataca cacgcatcat atcccgtact
    21721 gtctttgctg acaatccccc ttgtccattc agccgaccga attgcatctt gctttgaata
    21781 aagctgttta atttttgcgt ggtcaggctg gaaaaatatt cgccgcccag tgtcggcaga
    21841 atgtgctttg caacgatatt tgcgtaattc gcatagcttg ccggcttcac acgcagcttt
    21901 gccgctgcaa gccattgttc tgccgcatcc cgaaccgtaa gttttaagtt ggaaactgtc
    21961 cgctcttgac gaacagtgta gaactgtgtc attttttctt tcacaccaga tagtgtgcgg
    22021 tcatagacag atttgtactt cagttttccg tcttctttat aaccaattgg tacacgacct
    22081 tcgtagcgtc catctttgcg tttgtaaata ttacttcctt ttcgagccat aaagcaaaac
    22141 ctcctgacaa tttttcagac ggcgaaaccg tccgattcta acgtgaaaaa cagcttttcg
    22201 ttgccctgtt cacaaaaaaa tttggaaaac ctctttacat ttcctcagaa tgtggtataa
    22261 taatagcaaa aaccgcatga tcttgtcccg aaaaacgtga cgtaaatggg cggaattgac
    22321 gaaagcttgt ccttttcttt ttaaatatta taccatgctg gaatttgttt atacagcttg
    22381 atttttggtt gattttgtgg tatgatttgt aaaaagaaag aggggcagat acaaaatgat
    22441 tcggattgct attgtggatg atgaaaaaga acaggttgta atgctctcaa cctgtgtgtc
    22501 agtttttttt caggaaagaa atctggaata ccggattttt ccattttttt gtggtgaaga
    22561 tctattgtca tgtaatatcc cgatcgatgt cgcatttctg gatattcaga tgaatggcat
    22621 taatgggatt gaaaccgcgc aacgactgcg tgcctggaat aagtgggttg cgctgatcta
    22681 tatcacatcc tacgacagct atatccagaa ggcaatgaca attcaccctt ttgcatatct
    22741 gacaaaaccg acggagaaag ttgcaatttt tcaagtgctg gatgagtatc ttagtttcca
    22801 gcgcagcatt acaaatgcga tgcccaagga atattttcag ctgtcaacag agaactcctg
    22861 taaatatgta gaaatgtctg atatttatta tttttgctat gtgcaggatc gcacggttcg
    22921 ggtgcaaatg cgaaaagaat attatatcat caaagacagt atcacaaatg tatatggtat
    22981 tataaaccac gattactttc tgatgccgaa tccgagtttt ttggtaaaca tccagcatat
    23041 tcgggagatt gacggaaaaa ataaaaagct cgtcatggaa aatgacgatg tgattctgat
    23101 ttctcgcaga agatatggag aagtatttga tgcgttaaat cgttatatga caaatggaga
    23161 tttttaacta tgcagtatat tttagattgt gtgactttac ttttgaatgt cgttctttgc
    23221 ttttatttat tatatgggaa atatcctgtt cgttccaccc acatggtaaa acgaatcttg
    23281 ctgatcacag cagcagtgat ttcaaaagct ttaattgtgt atttacagat ttcaccactg
    23341 aattttatct caggatgctg cattattttg ctaataattt ggctgttgtt ttcttgcaat
    23401 aaagctgctt tatttatgta tacagcgtta tttgcaatgc tcacattaag tgcagatgca
    23461 ctgggtgtta ttgttgtatc agcatttcac caaaatacaa tttcaataac attaggaact
    23521 acagcgcttg tctggcaaca tcatatctgg aactggattc tccagatcgt ccttacaaga
    23581 attgcaaaga ttttgattcg ttccaaggaa caatttaaga tccggtggca cgaggtcatg
    23641 ttctatgtgc tggtggtgct gtttgaagtt gggatttttg catatttttc tgctacagca
    23701 caggaaaaat cctccggttg ggctatgctg ttgtttttag cgggattctt aggactggat
    23761 ttgtatatta tttatatatt ccataaaatt tctcaaatgc gggagaaaga acgtcagact
    23821 gacctgatgc agcagcatca gcagctccag ctgcaaatgt accgggaatt acagaagctc
    23881 tatcagcaga catgtgaaac ggcgcacgat atcaaccgtc acattacagc cttacaggca
    23941 tggatcgcgg gaaaatccac acgggatggg gaacaatatc tttccgatct gtccgcggcg
    24001 gcgaagcaac tacagccgag aattcggaat cagaatgcaa ttttagagat aatcctgaat
    24061 acagccgctc ggcgctgtga acaagaggcg attcagctca gtctggaaat tgaggatttt
    24121 cccatggaat ttttgtccga tatggatacc acgacaattt tttctaatct gctggataat
    24181 gccatagatg cgtgtatgga actggatcgt tcggtaaaac gaattgaggt ggttttgtgt
    24241 cagaagatgg gacttcttgc cctacgaatg acaaatactt gtcaggcagc agcggatctt
    24301 tctccgcggc aatggcattc caccaaaaag gatcatatgg gaattggctt atccaatgtg
    24361 cggaaaactg tggaaaaata ccatggtgtg gtatccgtca ggacgaaaga agaattgttt
    24421 caggtttccg tgacgattcc gatcaaatct tgaaatgccg gcaggaaagc cggtattttt
    24481 ttgtccgttt gaccatttga cgtaaaaaaa cgaccatttg acgtaaatct cttgtatttc
    24541 caaatcaggc atggtatact gcatgtgcaa gatatcttga aattatgaac aggaggtcat
    24601 gaaaatgcga gagaagaaaa gcttgaaaaa aagtttgctg acaggtgttg ctatggctgc
    24661 aaagaaaact tcgcagatca gtgccggaca ggcttgtatg tggtgggact atgaaccgaa
    24721 aatgccgaag tctgtaaaga agctacgtaa attctgatgc tggaacattt gtctaaaagg
    24781 tttgcaggga aattaatctg tgctggaatt atttccgaag cagatgcaga cgtgtacgtg
    24841 tacggatttt ttcagggcat gatgctgctt ctgaacgtga taacgacgtt attgttgggc
    24901 atattgtttc aaaagcttct tctctgcatg ttgttaaatg cagcatacat accaattcgg
    24961 atcagcgctg gtggtcatca tgcagacagt ccctttcgat gctatatcaa ttctaccatc
    25021 atgattgcga ttttgcttgc ggtaattaag tggattccaa ttaatccgat tgcatccgtt
    25081 attttacttg tggttgcaag tattgtcatc tggattctgg ctccggtgga aacggaaaat
    25141 aatccattag atgatacaga acaatacgtg tatcgtaagc gaacaagaat tgttctggga
    25201 attgaagcgg cggctagcat attctcttta atctttttga aagaacagat tgccgcaaca
    25261 attgtactgg gattgtctac agaagcatgg atgattatca tcggtgttgc gatgaatcat
    25321 aaaagaaaaa caacaactat atgaacgtaa aatgcaaggc tttatatcta tactttgggt
    25381 ataaaatctt gcattttttg aaacatgggt tgtcgaaaaa agtatataac atgaaaatta
    25441 aaaaggagga tattgatggc taaaaagtta acacgcacat tagccggagt catgtctctc
    25501 atgtttgtcg gacaaatcat ggtttttggt gacggtgcat ctcaaggcat tctgcacgcg
    25561 gatactatcg cttctgcagc agaagcaatt gaaggcatga aaaatgctga tcagttggcg
    25621 gaagaatttg aagaagcgac aaagggactt ggcgaggtag attattttga gctgccggaa
    25681 tcggttgagc agaatatcat gacgtatagc agccgaacag aagcacaggt tgaagctgaa
    25741 actgagtctg catctgattt tacatgggat ttcactgcaa aatcatatat tcagtatttg
    25801 aaagaagcaa caggcgttca ggatacatct gccttaacta ttaccggtaa agtacagaaa
    25861 ggtacagtaa acggtcatgc tgcttccgac aatacaccga tttatgtgcg tatttttgac
    25921 ggagattgga atgaaattga aagctgggag gtttccgaag gcgaagaata cactgcaact
    25981 gtaactggct ccggcgtgca tcatgtgaag tttgagtgcg atggttatct gccgttttac
    26041 ctgaaggact tcggaacagg ttcgttccag attggctctg gtgattcgct ggatacggta
    26101 acacttgtgc ccggtgatac gatatggaat gaggacaatg caaaccaatg gagcgacgat
    26161 gtaatcaata gcagcgattt ggcatatgtg caaagctgct acggcgctta tcgtggtgat
    26221 cctggattta atccaagtat ggatcatgat ggagatggga ttgtaggcga tgccgattgg
    26281 gattattttt atagattgta tgaagaactc gatgaaaatg agttctatga tatgaaccag
    26341 ttggatatat atcaatatga cttggataat aacggagtta ttaactacta tgacttacag
    26401 ttaaaacaga aagatacagg tgcaacagag gcggaactgg ctgattttgc cagcattgtt
    26461 aaatcagcac gggattacga gtcccctgca tttgtctata accattacta tacaaatgat
    26521 ggaaaggtag atgcagagga ctacaatgaa ggcattgaca aaataaatga acaaattaaa
    26581 ttgagagatc gctccagcaa ttactatgaa tacatggata aggacaacag tggcatcatt
    26641 gacgatttcg atattaattg gttttcagat gctcaaccag aaaccggaag tttggactgg
    26701 gatcatgcat ttaagagaaa tctgacaatg ctgtcagacg gtatgttccc ctacagtttc
    26761 aatcttcatg ataccaattt tgacctgaac ggctgcgctc tttatgttgc agattgtatg
    26821 tccttcacaa ccgatatgcc gcaattctgg tctggaaacg gcgcaacatt ggatattaac
    26881 ggaggagcac ttctgattga gaataatctg gtatttcgta ccgcatcgcc ggatggctgg
    26941 ggtggtaata ccggtcagct gatgaatctg aacggcggta tggttgccat tggcaactgc
    27001 tttgattttg gtcaggcaaa ctgctatgat acaattgagt tgaccaatag cgatgatacc
    27061 ttgatggtcg gtggaaactg gacatacatc acattgactg acatggaagg caaatggaca
    27121 gccggaaata tctggttcct cggaccaaca tgggaagtaa acgaagcttc cggcgataag
    27181 tctatctatt cttctggtac acagacaatt tattttggtt atgcaggcgg caaacagact
    27241 gttctttggg acaactgtga aacctacatt aacaatgaag atggttcgct gaatacagag
    27301 cgcacgttta attttggcta tgtggatgaa gaaggttatt gccagggtat ttatttcctg
    27361 aatgatttca cagaagaaaa ctactggttc agaccatggt tccgcccgta tgatattccg
    27421 gattacaccc tctaccgtaa gggctgggag attggcgatg gtgtccatat tgcaactggc
    27481 aattacacaa aatcttttac agatttgagc gtaacttctc ctggtgtcac ttccgatttt
    27541 gtccgcactt acaattccat gagtgatgag gaaggcagct ttggtattgg ctgggatttc
    27601 aatatcgatg tgagtaagat cgtaattccg gcacagggtt actatcaggt agttcttcca
    27661 gatggatcta acaccacatt caaggatgat ggaaacggcg gatttgagtg cctgaatgca
    27721 cacagcacca tgacaaagtc cggcaatgaa tacaccatca caaatgccgc tcagtctcag
    27781 tatcacttca atgcagacgg cgaactggat tgggtcaagg atgcagaggg caatattctg
    27841 accatctctt ccatgtccaa caaccagaga attgtaacgg attccacagg cagaacttac
    27901 acgattactt acaatggcaa taaagaacat tctcgcatta caaaaataga agataccaca
    27961 gcagaacgta ctgtcactta cgaatacaac aatgatttcc agttggtatc agcaaccagc
    28021 gtttctggcg gtactgaaac atatgagtat gatgggaacg gcagactttg caaaatcacc
    28081 aactgctacg atgaaatgac agaccagatc gtgtacaacg agaatggttc tgtgaactgg
    28141 ctgaccaatg catctggtct gaaacaggtg tatacctatg ataaagtaca gaaacaaaca
    28201 ggactgaaag agtatgatgg cgatacgctt atcaagactt ttacatatga ttatgatgaa
    28261 aaatatgccg tcaagacaaa tactgttgaa actgatggtc aaacatacga cgtagataaa
    28321 atcacctata acatgattga tgacgaaaac aagtatgacg aaatgtctga aagcgttgac
    28381 atcatgggta atacgacaaa gtacgaccgt gatgcgaatg gcaatgtcat caagaccacc
    28441 aatgcagatg gcacatacac acttgccaat tacaatgata aaaacagtgt tattgcagaa
    28501 gttgacgaat ctgggaatgc aactatcaaa gcatatgatt cgaatggaac tcggcttttg
    28561 aaagaagcaa ccagtctgca tccattatct caaactgata tcaatacagt tacagcagac
    28621 aattttgatc cagtgaagta tcttgcagcg aatgaggcaa gttatgcaat cacgagtcat
    28681 gaatattatg ctgatagtta tgtgagtgga attgcagggt tgattcgtgc tacaaccgac
    28741 cccgaaggta atgtaactga gtatgattac tacaaggatg gcgttggaaa aggactggta
    28801 aaatccaaga cactgaagga tggaaatact gttgtaaata cagtttctta tgagtataat
    28861 gcacaacttc aagtgtcaaa ggaaaccacc agttttgaca tttccaaaaa tctttattct
    28921 gtaaaggaat atgagtacga taagttcaac aatgttacag ttactagaga ttacggaacg
    28981 ggaagcacac cggcgactac cgttgcagaa tatgatttgc tgagccgcaa aactgcggag
    29041 tatgcaccga attattctgc ggataagagc catggcagct tgacaactta ttatccggac
    29101 ggaaacaaaa aatcagaaac gaatgcagaa ggtaatatta cctcttatgt gtatgacgct
    29161 tacggacagg taatcaagaa aaccaatcca gacggcacca tgaatctgac ggcgtatgat
    29221 ggtcttcaaa gagaaaaggc aacatatttc ttgggcagtg aaaatggcac aaagcaaatt
    29281 ttaacgaaga catcctatga atttgcagga tacaattttg acatttattc tgcacttgat
    29341 acttcagctt ctcactcctg taagggactg aaaacaacca aaacaaccta cattaccg
//
LOCUS       DAWBGR010000008        28749 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_636_length_28749_cov_4.951384, whole genome shotgun sequence.
ACCESSION   DAWBGR010000008 DAWBGR010000000
VERSION     DAWBGR010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 28749)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 28749)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28749
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_636_length_28749_cov_4.951384"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    16057..16066
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    23297..23396
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7115 a         6755 c         8104 g         6665 t
ORIGIN      
        1 gaaaaatttg acgacgcatc tgtcgcacaa tctttgtgcg gtattgccct aaaaatgttt
       61 gagaaaacgc ttgctttttc cacggcaatc tgctataata agaaatagca gagaatactc
      121 tgcggaaacc aacacggagg tgaatttctc atggcatata aaattacaga ttcctgcatc
      181 agctgcggta catgtaagga cgcatgtccg gtggacgcaa tctctgaagg tgctgacaag
      241 tatgaaatcg ctgaagatgc ttgcatttcc tgcggttctt gtgcatcgga atgtccggca
      301 gatgcaattg aagctgacta agcttttgta aacaaacggc aaaaaagctg ttccgttccg
      361 gcttcacact ggaaacggac ggctttttct tttagataag gaaagggacg gatttatgat
      421 gaaaaagcag atgcagaaag cggtctgtct gctgttgggc agtgtactgc tgctgtctat
      481 gggtgcctgc ggcaaaaaag acaaaacttc tgcccgcgat gtgactgacg gttcttctgt
      541 aacggcaacc cagactacac aggttgtcag agagatcaag agccataagg gcgagatcgg
      601 tgaggagatg cagtatcagg atgtagatgc cgtgatgaaa aaagcatacg tttctgacta
      661 cacattctcg gaaaacggca gcgagatcgg tctgacattc ttcgaatttg agatcaccaa
      721 caatagtgag gacgaactgg cggtggactt gctgtcacag acattcggtc tgacggtaga
      781 cggcgaggct taccccggtg tcagcattcg cggtccgcgg tttatctata aggaattcgg
      841 cgattcgggc aagacgttcg acaccgcaat ccagcccgga gaaacccgcg acggctatat
      901 ctgcatggaa gtgccggcag atttccagag ccttcgcatc acctatttcc ccagagccgg
      961 ctttttggac tgggacgagg cattcctctt tgacatcgat cgttcggaca tggaagctgc
     1021 accggagccg gtggaagcat tccagtaaga gcctgcctgc aaacgggctt tgtgcggtgt
     1081 tgcctcagat acaaaaagca aacggcaggc agatacaaat gcctgccgtt tttcttgcac
     1141 agaacaaagg caataccgca caaagtttac cggtcagttt tgcatgcaat tcccgaaaaa
     1201 ttgaaatgtt tatctgacgc atcatgctgc cttgcaaaaa aaagtttagt ttttcagaac
     1261 ttacacttga cttttgcaga acattctggt acaatccggt ttgacaaaag aaatttgtca
     1321 gaagggagta tcgaatgaga aatatgcaaa aaatgtggga agactatgca aaggaagtgc
     1381 aggtgctgcg ggaacgcatc gcagaactgc gggaggtctt gcagcgggac aagacgatgg
     1441 caccgctgga acggcaggaa ctggaggcac ggaaacgtct gctgagtgaa gaagtgcggg
     1501 atatggaacg gagcatggcg gacattgcag tctatgccga aaatgaaaag gcaggcgaat
     1561 cggcatgtgc atgacaaaag ccgcctggga acagatgttg ttttccgtgg attttcatct
     1621 ggaccggcag gaaaaacaac gtgccgcctg tctggctgcc agaaacattc tggaacatga
     1681 catttctgca agacagaaac aagtcctttt gttgtactat ggagagggaa agaaaatggc
     1741 ggatattgca cagctgctgg gggtgaacat ttccacggta tcccgcaccc accgccgtgc
     1801 ctgtgccgcc gttcaaaagc ggctgaaagc ctgcgggctt ttataagagc ctgttcagta
     1861 ttttttcgca caaaatcatt ggagaatcga tcatgaatac accagtctat gactttttga
     1921 tacaatacgc agagcgggac cctgtccggc tgcatatgcc cggacacaag ggcagattcg
     1981 gggcaccggc gttgcagcgt gccacgacac tggatctgac ggaaatcacc ggtgcggaca
     2041 gcctgttcga agccgagggc attctggcag agagtgaacg gaatgccgct tcgctctatg
     2101 gaacggcgta tacgttttat tcctgcggcg gctcgaccct ctgcattcag accatgcttt
     2161 tgctgatgaa acagcagggg cgggcggtgc tggcggcgag aaatgtgcat cgggcatttt
     2221 tgaatgcctg tgtgctgctg gacctgccgg tgcagtggat ctatccccgc gaaagtgacg
     2281 gcattttgtc cggtacctat gatctggcgg attttgaggc cgcattgcag cagcagaccc
     2341 gaccggcgtg tgtctatgtc acttcgccgg actatctggg gcgtatggcg gatattgccg
     2401 gtctggcgga actgtgccac cggtatcacg cgaagctgct ggtggacaat gcccacggag
     2461 cacatctggc gtttctgccg tggcggtgtc acccgatcca attgggggcg gactactgct
     2521 gcgactctgc acataagatg ctgtcggggc tgaccggtac tgcctatctg catgtccgca
     2581 gcgatattga tgtggaaccg gaacgggtgc gggacgcgat ggcaatgttc ggctcgacca
     2641 gcccctcgta tctgatgctg gcttcgctgg actggtgcaa tcgggaactg gcttcgccct
     2701 tgttccggaa acagctggag gaagtgctcg accgcattca gcagctgaag tgccggtttt
     2761 cgccacgtta ttgtttcgta gacggcgacc ccatgcacct gactatagat gcggcggcag
     2821 gcggatttca cgggctggaa ctggcggcgt atctggaaca gcatgaagtt tttgtggagt
     2881 atgccgatgc atttcatgtg gtgctgctct gttcggcagc caataccgat cgggactttg
     2941 acattctgga gcagctgctg gaaaggtttt cgcctacacc ggcggaatcc ggagaaaagt
     3001 acgctctgcc cagaccggaa aaggtctgcg ggattcggga ggcggcactg gctccgcagg
     3061 agatcgtgcc catcggggag agcagcggac ggatttgtgc cgcggtgaaa gtgccctgtc
     3121 cgccggcagt gcccattgtg cttagcggag aacgaatcga tgcccgctgc attgcggttt
     3181 gtgaggctta tggaattacg gagatcagcg tcgtacagta ggaggtggcg gcatggcaca
     3241 gttttatggc aatgaacaga tacagcacac cctgcaccag atgcttttgc gggacagggc
     3301 tccccacgga ttcctctttt acggggaaac cggtctgggg aaaaagacgc tggcaaagca
     3361 gtttctggca gaactgctct gcacaggaac ggaaaagccc tgcggcagct gcaaatcctg
     3421 caaaatgctg gcggacggtg tgcatccgga cgtgcggctt gtggaacaca gcggcaagcg
     3481 aaacggcttt tccgtggaca cggtgcggga ggtgtgcgtg gatctggcat cgccgcccaa
     3541 cgagggcaat gccaagtgct atgtctttgg ggactgtgat gcaatggaca cccgcaccca
     3601 gaatctgctg ttgaaagcgg tggaagaacc gccggcttat gggtatttta tttttacggc
     3661 aacttctccg gcatcgctgc tgcctacggt gcgttcccgt atcgtgtcgc tggcggtgtc
     3721 gccggtgtcg gaacaggaat gccgtgctgc actggcggaa cggggctttt cgccggaaga
     3781 tgccgcagaa gcggtggcgg cgttccatgg gaacatcggg cagtgcttaa attttttgga
     3841 aaatatgcca attcgtgagg ccgttggatt gacaaaaacc gcaatcaata gtataataaa
     3901 taagaatgag tatgcgttgc tgcaaaccgt ttcggtgctg ggaaaggaac gggaacggac
     3961 gtttttgttc ctgaacctgt ttgaccggtg cattcgtgat gccattgtgt ggaagcatga
     4021 cccctgtgcg gcgggaatcg gctgtgatgc agcagggtcg aaaaagctgg cagaacggct
     4081 ttccgacagt gccggacagg caatgcatcg tgctgtggac agtacctacg gggcaatgga
     4141 ggcaaatgtc aacctgccgc tggcactggc agctttttgt gcagactgca tggacgcgta
     4201 acagcttctc attccggaaa tgaaatttcg gggcgttggc tctactatag aaaggaagaa
     4261 agtatggttg aaatcatcgg cgttcggttt cagaacggcg gaaaggtcta ctatttctct
     4321 ccggacagct ggaaggtgaa acagggagat cttgtcatca ccgaaacttc ccgcgggacg
     4381 gaatgcggag aagttgcgat cgccaataaa cagatcccca aggagcaggt gacagtgccg
     4441 ctgaagccga tcctgcgggt cgccacgcgg gaggatcgcc ggacactgga acaaaatcgc
     4501 aaaaaggaag aacgtgcttt tcaggtctgc cagcagaaga tcgcctatcg aaagctgaaa
     4561 atgaatctgg tagatgtgga atgcacattc gacaacagca agctgctgtt ttatttcacc
     4621 gcggacaacc gcgtggactt tcgggaactg gtcaaagatc tggcggcagt gttccggaca
     4681 agaatcgaac tgcggcagat cggcgtgcgg gataaggcga agatcctggg cggtatcggt
     4741 atctgcggca gagaattctg ctgcaaggga cacttgcagg atttccagcc ggtttccatt
     4801 cgcatggcaa aggaacaggg gctttccctg aatccgacga agatctccgg ctgctgcggc
     4861 agactgatgt gctgtctgaa atatgagcag aacgtctatg aggaactgct ccgcattacc
     4921 ccgaaggtcg gggcaatcgt caccatgaca gacggcacca gggcaaaggt ctgcgaaacc
     4981 aatccgctga ctggcgatct gaaggtgatt tcagaaaaat ccgatgtgcc ggtaaaggtc
     5041 aagcgggacg acgtgaagct gctgcgggac ggaaaaatca aggtcgatgc ggcagaacgc
     5101 aaggcattga aatcgttaga ggataagtaa ggattcaaat tcgggcagac cggctcccca
     5161 ccggtctgca tataggaggg attttttatg gcatctcaaa gtaaacgccg ccgcgtccgg
     5221 tatgaccgga ttctggcagt tcttctggtt ctgatcctgc tgatcgtact gctggtgaag
     5281 tgctgttcca gctgctccag caagaaagac aagaagaacg acaatgtgac taccacacct
     5341 actcaggcga ccacagctcc cacagaggca gataacacga aaaccgttta tctgagcccg
     5401 tccaatcagg aggacaacaa gttctatgtg ggcgacacca acgaagctgc ggtctgccgc
     5461 attctggcac aaaagacggc tgccattctg gaacagtccg gcgtgaacgt catcgttgcc
     5521 ggcgatgctg acagcattca gaccaagaca gcggccggcg atcagaatct ggcggcatat
     5581 gtggcgattc acaccaacgc cggtaccggt gtcggcacag actgctatta caatgccaat
     5641 tccgaagcca gcaagagtct ggcacaggca gtatatgatc cggtggcagc cctgactccc
     5701 acagatgaca agggcttgat cgacggttcg gacagcaatt ctgaaaatta tcagtatgaa
     5761 gtgggtgcca acaagagccc cagctgtctg atcgaagtgg aatatcacga cggtcccaat
     5821 gtggcacagt ggattctgga caatgaggac aacatcgctt ctgcactggc acagggtatt
     5881 tgcagctatt tgcaggtagc tactcccaca agcggtgcag cagtgaccac cgcagcactg
     5941 ggcggtgatg tgaacagcac gacaacggca gcgatcggcg gagcagatgc tctggcaact
     6001 actgctgcat ttggtgcaga gggaacaaca ggcacagaaa atgcgatcca aaatcaatta
     6061 tcagctggaa actgaccgga ttctggcaca gctgccggcg gaacggacac cgacactgtt
     6121 tctgcatgcc tgctgtgctc cgtgcagcag ttatgtgctg gaatatctga gccggtattt
     6181 tgccattacg ctgttcttct ataatcccaa catttcgccg gaggcggagt atctgcaccg
     6241 gaaagctgag ttgtgccggc tggtgcagga attaccgctg gggaatccgg tgacggtggt
     6301 ggacggcgat tatctgccgg aacggttcta tgctcttgcc aaagggctgg agcaggagcc
     6361 ggaacgggga gcacgttgtc agaagtgtat cgggcaccgt ctggaagaaa catttcgggc
     6421 ggcggcagca ctggaagtgt taccggattt tgtctgcacc acgctgacca tcagccccca
     6481 taaagacgcg gtgtttatta acaccagagg agcggaactg gcggagcagt acggcatccc
     6541 gtggctgccc acggacttca agaaaaaggg cggctatcag cgttcgatcg tgctgtccag
     6601 agagtatgac ctctatcggc agaacttctg cggctgcgtc tacagccggc tggaggcgga
     6661 acggcgggaa aaggagatgg cttcttcatg agcggctgtt ctttttgggg gaacggtgag
     6721 agacagggaa tccgttttgc gtgaagatgg gggttggtga ttttgaaata ttcagaaaag
     6781 acggaaaaga aaatcaaaac gatactggga ataaccacag catcaatctt ggcacttctt
     6841 tttctggctt atatgtttct tcgcatcctg ttttgggcgg aaacgggcag cttttttcct
     6901 actgttcaga agagccgttc ggtggaaatt gcaatttcgt gtgacccttt tgatgtattg
     6961 gcagtaagag aggaactggc accttataca aaggattata caaccgcagg cgttgagatg
     7021 atatatgcag aatatcattt cagtgaagat ggtgttacgg cgatgtatgg attcactggg
     7081 gggaaatatc gactcggaag aggagaaacc atcagttttt acctagatgc tgagaaaggc
     7141 actgtggaag agatatctta ttatgcagca ggggctaaag tgttttccag ttatcccagc
     7201 cattacttcg atgcggatgt aaacatgatg gaggaatacg aaaaaattaa aaataatccc
     7261 tctgattttg atacaatcaa agaggtcgag ttcaccctgt ccaataacaa ttgtgagcca
     7321 aagctctatt ataattgatg caaaaccaca ttttgtgatt tgggggaatg aatccctttg
     7381 gacagattcg gggaatcaaa atttccgggt gcgttttgtg caaaaaaaca gttgacaagc
     7441 ttcggcgatt gtgctatact agtgcttaga gcgttgaagg gaacggcaca tttcgccgaa
     7501 gctttttgca gagaaccgct gtcaccggct gaaagcagcg ggaaaaagtg aagaaatttg
     7561 cacaccactc cctgagcctg cccaatccgc agcggtctgt ccccgttatc ggacaccacg
     7621 aggtgtatgt gcctttggca cgtgcatgaa acaagggtgg aaccacgata gatttttcat
     7681 cgtcccttgc acagtccttt actgtgtaag ggactttttg catttacttt gggaaatgcc
     7741 gcacacggca cgtctgacat gtcgggcgtt tcccgcattc tggaaaggaa gatggatcat
     7801 gatcaaactg acattgaaag acggcagcat tcgggaactg gaagctccca cagctgcggc
     7861 agaagtcatt aagggcatcg gcatggggct gtacaaggct tcctgctgtg tcaagctgga
     7921 cggagaggtc tgtgacctgc gtactgtcat cgatcacgac tgcacatttg aagtcatgac
     7981 cttcgacagc gaagaaggta agaaaacatt ctggcacacc gcttctcacg tgctggcaca
     8041 ggctgtcaag cacctgtatc cggaggcaaa gctggcgatc ggaccggcaa tcgataccgg
     8101 tttctattac gactttgacg tagaacatcc gttcagtcag gaagaactga ctaagatcga
     8161 agccgagatg aagaagatcg tcaaggcaaa cgaaaagctg gagcagttct atctgtcgcc
     8221 ggaagaagcg gtggctaagc tgaaaggaat ggacgaaccc tataaggtgg agctgtgcga
     8281 ggagcacgcc ggaaacggtg agccgatctc gttctatcag cagggcgatt tcacagatct
     8341 gtgtgccggt ccgcacctga tgtctaccgg tacgatcaag gcattcaagc tgctgtcctg
     8401 caccggtgca tactggagag gttcagaaaa gaacaagatg ctgtcccgtg tctatgcggt
     8461 ggcattcccc aaggcatcga tgctggaaga acatctggca aagctggaag aagccaagaa
     8521 gcgtgaccac aataagctgg gacgggaact ggaatatttc accactgttg acgttatcgg
     8581 tcagggcctg ccggtgctgc tgcccaaggg tgccagagtg gtacagctgt tacagcgttg
     8641 ggtagaagat gtggaacagg cacacggctg cctgctgact aagacgccgc tgctggcaaa
     8701 gcgggaactg tacaagattt ccggtcactg ggatcactat ctggacggca tgttcgtact
     8761 gggcgaccct cacgacgaaa ccaaggactg cttcgctctg cgtccgatga cctgcccgtt
     8821 ccagtatcag gtatatctga accggcagcg ttcataccgt gatctgccga tgcgtctgac
     8881 ggaaacttct acgctgttcc ggaacgagga ctccggcgaa atgcacggcc tgatccgcgt
     8941 tcgtcagttc accatttctg aaggacacta tatcctgcgt ccggatcagc tggaagatga
     9001 attcaagaac tgcctgcaat tggcaaagta tttcctggat acagtcggac tgctggacga
     9061 ctgcacattc cggttctccc agtgggatcc ggcaaatccg ggcaacaagt acgagggtac
     9121 accagagcag tgggaagaag cacagcgtgt catgggcaac attctggatc acttgggcgt
     9181 gaagtatgag attggcatcg acgaggcggc tttctatggt ccgaagctgg atattcagta
     9241 caagaacgta ttcggcaagg aagacaccct ggtcaccatt cagatcgata tgctgctggc
     9301 agagcgtttc ggcatgtact acaaggataa ggacggtcag aagaagctgc cgtacatcat
     9361 tcacagaaca tcgctgggct gctatgagcg tacgctggct tacatgatcg aacacttcgg
     9421 cggtgcaatg ccgctgtgga tcgctccgga acaggttcgt ctggtgccca ttgcagaccg
     9481 tcatctggac tttgcatatg aggtcaagaa ggaactggaa gctgctggtc tgcgtgtcga
     9541 ggtggacagc cgtgccgaaa aggtgggctg gaagatccgt caggcaacga tggaaaaggt
     9601 gccgtatatg ctgactgtgg gcgacaagga atgcgaagaa aagacagtcg cagtccgcaa
     9661 gcgtaacggc gaagatctgg gcagcatgac ggtggcagaa ctgacagcaa agctggtcga
     9721 agaagtccgc accaagcaga agtaatcaga caatgcagca gaagctgctg cataacagaa
     9781 cagaaaaagg cgaacgtttc ggcgttcgcc ttttttgctg gtatttcgcg aaaaaattga
     9841 aaaagtatac attttccaat tgacaaaaca tcatttttca gttataataa aaagcaggta
     9901 tattttgttt tgaaagcgag tatttggaaa atgggaagta cgaaaatcaa tctgacggag
     9961 gaacagttca cagaagtgac actgtccgcc ctgaaagagc acctcgaggg aattacggaa
    10021 tatccgattc tggattttga tgatcgtgca ctgaggctgt tgtccgccgg cgttgcggaa
    10081 acgctgcggt acagtgagat caagccccgc ggcggtctgg cataccggtt cctgaagcgg
    10141 ctgtttgaca tcgtggtgtc tgcactggca ctgatccttc tggcgatccc catgggcatc
    10201 gtggcactgg tgatctatct ggacgacaag ggatcgccaa tcttttcaca ggcacggctg
    10261 accaaggacg gaaaggtgtt ctacatgcac aagttccgtt ctatgtgtgt cgatgcggaa
    10321 cagaagttcg cagaggtgca gaaagaaaac gaaaccgacg gtctggcgtt caagagcgag
    10381 aacgacccgc gaattacacg gatcgggcat tttatccgga gaacgtccat tgacgaactg
    10441 gcccagctgt gggacgtttt ctgcgggcac atgtccatca tcgggccgcg tccgccgctt
    10501 ccgcgggaag tggtgctgta tacgccccac cagatgcagc ggttacaggt aaaaggcggt
    10561 ctgtcctgta tttgtcaggt agagggcaga agcgatatgg gattcgaaaa gtgggtggaa
    10621 tccgacctcg aatacattca gacacgcagt gccggactgg atctgaagct ggtgtttaag
    10681 accattggcg tggtaattat gaaaaaaggt gcaagataac cataggaaga aagagccgct
    10741 gtgcagatgc atggcggctt ttgtgctgtc cggaatcttt ttcggaatct gccttgtcaa
    10801 tgccgcccgc tttatgttat aatagaaaca gcatcacaac aagcgaggtg aaaagccgtg
    10861 tgcctgtgcc gcggcgatta tgaaacgatt ggtagtgggc attactgccc atgtggattc
    10921 cggaaagacg acgctttccg aagctctgct gtaccgtacc ggcgagatcc ggaagctggg
    10981 gcgggtggat caccagacag cttttctgga cacccacccg atggaacggg atcggggcat
    11041 tacgattttt gcacatcagg catgtattgc ctgtgaaaat gcggaatata cgctgctgga
    11101 tactcctggg catgtggact tttcagcaga aaccgaacgc actatgcagg tgctggacta
    11161 tgccattctg gtcatcagcg gcacagacgg cgtgcagtcc cacacggaaa ccctgtggaa
    11221 actgctggaa cgctatcagg tgccggtgtt cctctttgtc aacaaaatgg acttgacagg
    11281 ggcagacaag gcggcagtgc tggaacagct gcaaagccgg ttgagtgacc gctgtgtgga
    11341 cttttccgca gacggcaccg atgcctttta tgaacaggtg gctctgctgg atgagccgtt
    11401 catggatact tatctggagc aggggaagat cccgccggaa ctgctggcaa aaggcattgc
    11461 ccgccggaag ctgatcccct gttacttcgg ggcagcgttg aagctggagg gcgtggacgc
    11521 atttctggag ggactgcacc ggtatacgct ggaacagccc tgtccggcac agttcggggc
    11581 gagggtgttt aaaattgccg ctgacgaaaa ggggaaccgg ttgagttacc tgaagatcac
    11641 cggcggacgg ctgcgggtac gggacagcat cgcctatact gcttccaacg gcagggacat
    11701 gcaggaaaaa gtcagccaga tccgtgtcta cgccggtgcg aagttcgagg cgaaagaggc
    11761 ggtccctgcg ggcacggtct gtgcggtgac aggtctgagc cggacgttta tcgggcaggg
    11821 gctgggcagt gccggcgacg gtatggctcc ggtgctggaa ccggtgctcc gctatggcgt
    11881 acagctgccg gaaggggtgc accctaccga tgccctgaaa cagctggcac agctggagga
    11941 ggaagatccg gctctccacg tggtgtggaa cgaccacgcc ggacagattc agatgcagct
    12001 gatgggcgag gtgcagctgg aagtattgca gcggctgatc actgaccggt tcggtatgca
    12061 ggtgcagttc gatgccggac agatcaccta taaggaaacc attgccgagc cggtggaggg
    12121 cgtggggcac tatgaaccgc tgtgccacta tgccgaagtg cacctgctgc tggagccact
    12181 gccgcagggc agcggtttgc agttcggggt gaactgtcgg gaggacgatc tggaacgaaa
    12241 ctggcagcgg ctggtgctga cgcatttgga ggagaaaacg catctgggcg tactgaccgg
    12301 ttcacccata acagatatgc ggatcaccct gtgtgccgga aaagcccatg tcaagcacac
    12361 cgagggcggc gacttccggc aggcgaccta tcgggcggtg cggaacggac tgcggaaggc
    12421 gaaaagcatt ctgctggaac cgtggtacga gtttttgctg gaactgccta cggaaaacgt
    12481 gggacgtgcc atgaccgatc tacagcagat gggggcggag ttccagccgc cggaaaccga
    12541 gggcgaccgt tcggttttgc agggcagtgc tccggtggcg aagctgcggg gctatgcttc
    12601 agaggtgacc ggttataccc acggcgtggg cagactggtc tgttccccca agggctatgc
    12661 tccctgtcag aatccggaag aagtcattgc cgcagtgggg tatgactgcg acagcgatct
    12721 ggaaaactct gcggattcca ttttctgtgc ccacggagcc ggctttgccg tcaagtggga
    12781 cgaggtggaa cagcacatgc acctgcccag ttgcctgcac cagctggaag aacccgacga
    12841 accggcggca ccggtgcggg tgtcgcgggc agcctatggg ggcagtctgg cagaggacaa
    12901 ggaactcatg gcaatctttg aacggactta cggaaaaatc gaccgcaatc cccgacaggc
    12961 actctacacc cccaaagagg aggacaccgc gacagcatat cacggcaagc cggatccggc
    13021 gtatgacggg gaggagtact tgctggtaga cggctacaac atcatttttg cgtgggacga
    13081 gttgaaaacg attgcccaaa acaatctgga cagtgccaga ggacagctga tgcacatgtt
    13141 gagcaactac tgcggctatc ggaagtgcaa gctgattctg gtatttgatg cgtataaggt
    13201 caagggctat cacggcgaaa cagagcagta tcacaacatc acggtggtgt ataccaaaga
    13261 ggcggaaact gccgactcct acattgaaaa ggcaacgctg gacttgtcga agaagcacaa
    13321 ggtgcgggtg gcaacttcgg acggcatgga gcagctgatt attctgggga acggtgccct
    13381 ccgtatcact gcggcggaat tccggcagga ggtcttgcag accgaggcag ccattcggga
    13441 atatgcggca cagatgaagc aggggaaaaa gaccattacg gagaagcatt cgtcataagc
    13501 tgaggcagtg ctgccgtagc agaatacccg aaatccctgc ggaaaaatcg gcagttctgt
    13561 caattgtttg tgcagcagaa atatgataaa ataaggatat tccggagcgt gtttcggcac
    13621 gctgagatat agaatgaata tggaggcatg acaaatgcaa atgcagatac agcaccagtt
    13681 gcggcgtgtc gctgctgccc tgctgaccgc ggcggtcgct gtgggcacag gagccttttc
    13741 cggtctggca ttggaaaccg attccggcac tctggaaagc ttacaggcgt tcctgctggg
    13801 aaaagaaaat gccggcggaa cggactgcaa cggggacggc gttgtggacg gcatggactt
    13861 ggcactgctg cggcagcagc aattcgacgg gggagaaata caggacggtt atgccggctt
    13921 tatccgcaca gacggcaaac tgctggtcga tgaaaacggc aagcagtatc tcatcaaagg
    13981 catggcaatc ggcaatgatg tgtggtcgaa tcccaccaca gcccctgccc aggatcacga
    14041 tgcggcaagc tatcaggaac tggcggacat gggctttgac tccgtgcggt tctatctgaa
    14101 ctacgccatg tttgaatctg acagcgaacc ctatacctat cgggaagaag gcttccgctg
    14161 gctggacacc aacattgcat gggcaaaggc ggcaggaatc cggctggtgc tgaacatgca
    14221 ctatccgcag ggcggttatc agtcgcaggg gaacggcacg gcactctgga cagaaccgga
    14281 aaatcagaag cggctctgtg ccctctggac ggaaattgcc aagcggtatg cagacgagcc
    14341 ggtgattctg ggctatggac tggtgaatga accggtggta gctgctgcca gcggtgagga
    14401 gagccttgcc ctgtggcagt ccgtagcaca gatgctgacg gacgggatcc gtactgtaga
    14461 ccagaaccat atgatctttg tggaacgcat gtgtgcggca cagaatctgg caggcacaca
    14521 ggaacagtgg atcaatttca atgacgaaaa caactacgtg catctggacg atgacaacac
    14581 ggtgtacgag ttccacgact atgatccgca tgcttttacg catcagggct tcgactgggc
    14641 ggggacactg ggcaatgatg tgacctatcc ggacgaaagc tatctggtgt ccagcggcaa
    14701 tgcccagtgg ggcacttcca cctttgccgg cgatcgggcg gacacggaga acaccgagtg
    14761 gcagtatctg aaaagcggca ggatcacgcc gaaatcggac ggcaaacagc taatcagtct
    14821 ggtatttcag gcacagaaca tcggggacta cggctacgcc cgggcagatg aactgcggct
    14881 gaacgaatat gatgaggacg gcagctttgt ccagaccata tacgccaacg actttgacag
    14941 catgtccctg ctgaacttct ggtccagcaa cgactccggc ggacagtact acgcttcttc
    15001 cgagggacat ctggcaaagg gcagtctgat gctctccggc accaccgatg atgccaacac
    15061 cagtacaaga tatttccgtc ccacggcagg acacagctat gaggcatgcg gttattttca
    15121 ggtgaacacc aagaatgccg atgccattgt ccgtccccgc gtggacgtgt ggaatgtgga
    15181 ttctgtggaa gtgctgaatc gggactatct ggaaaagagc gtggcactga acacggcgtt
    15241 ttcggaaaag tacaatgtgc cggtatactg cggcgagttt ggtgccggtt cgcactgctt
    15301 tgagaacgac cgcggcggtg accgctggat cggggacatg ctggaaattt tccgtgacgg
    15361 cgatgtcagc ttcaactacc acgcctatca cgatggttcc ttcgggctgt atgagggcgg
    15421 cggtctgccc agtcccgcag ggagaaatga tacactatat caggtgctgg tagaaaaact
    15481 gaaaaaatat acggaatgac aggatttacg aaaggaactg atatgaaaga aaaagataca
    15541 gaaaagcagc tgaaagcctt ggaagaagct gtgcggcagg agcaggaaac cggcggaaag
    15601 acggcgaaaa agcatcggga aagcagctct gcggcggcga aagaatctca cccgctggct
    15661 tcgtttctca tcgggctggt actgctggga gcaggcatct tctgggtgct gaactccttt
    15721 gtggtctcct tctcgtgggg cagcctgtgg ggcggcttcg gcatgaacac ttcactggca
    15781 actggtctga tgctggtgcc gctgctgatc ggcatcggtc tgctgttctt cttggacaga
    15841 aagtggattg gctgggtggt gacggcaatc ggactgctgg caattctggt aacgctgctg
    15901 acttcggtgc ggtttcgtcc ggtgtcggct tcgctgtggc agtatgtggt catgttcggc
    15961 atgatcgcag ccggttgcgg tctggtggcg agagggctgc tgaaaagcag agattaaggg
    16021 caataccgca caaagagcgt ctggcgactt tgtacgnnnn nnnnnncgca caatctttgt
    16081 gcggtattgc ctaagggaac gcagaaaccg gctttgtacg acgtttcctg aaaaaggcgt
    16141 gtggttttgc ggaactgcac gtcttttttg tgagaatgca cggcttgcca accatgcgaa
    16201 atcatgctat aataaaaagg acggtataaa gtttacacat tgttttgctg cgggtgggaa
    16261 ttgacaccta tttaagccta tagcttgcct gcatctaaac tttatgcagt gctctcaaaa
    16321 aacgatcaca gaaacaggag gcacatcgat gaactatcga atatttctgg tggaggacga
    16381 ccgcagcatt gcggacggac tgcggcacca gctggaacag tgggattttg cagtacaggt
    16441 ggtacagaat ttccgcggcg tgctgggaga attcacggcg tttgacccgc atctggtgct
    16501 catggacatc atgctgccct gttatgacgg ctaccactgg tgtcgggaga tccggcaggt
    16561 gtcacaggtg ccgatcattt ttctgtcctc ggcttcggac aatatgaatt taattatggc
    16621 aatgaacatg ggcggggacg attttattgc caagcccttt gactggaacg tgctgctggc
    16681 aaagatacag gcactgctcc ggcgaaccta cgacttcggc gggcagacgg ctctgttgga
    16741 acatcgggac gcgatcctca acaccgcaga tgccgtgctg acctatcacg gagaacgcat
    16801 tgacctgact aagaacgagt accggatcct acaggctctg ctggaacaga agggcaaggt
    16861 cgtcagtcgg gaaaccttaa tggaacggct ctgggcaacg gacagttttg tggacgagaa
    16921 caccctgact gtcaacgtga accggctgcg gaaaaagctg gacaaggcag ggctgcacga
    16981 ctttatccgt accaaagtgg gcatggggta tctggtggaa taggaggacg gcatgaaaat
    17041 tttcggggat tatctccgcc ggcatctggg cggtatcggg atatttctgg catgctgcgg
    17101 catttttgtg ctggtatttt ctctgtacga cctgccggcg gaagccgtgc tgtacggcgg
    17161 tgtgctgtcg gcggtgctgt atttgttctg gtttttggtg cgtttccgga agtattacca
    17221 gaagtgccgc acactccggc agatgcagca ggagatcgtc attaccaccc ggcatctccc
    17281 gccgccggag gacgcgtcag aacaggcgta tcaggaactg gtggaactgc tgtaccagaa
    17341 gcagcaggaa ctgacacagg aatggcgggt gcggtacagc gacatgatgg aatattacac
    17401 ggcgtggacg caccagatga aaacgccgat cgctgccatg cggctgcacc tacaggcggc
    17461 ggacacgccg gaaaatcaca tgctgctgga agatctacag cgaatcgaac agtatgtgga
    17521 aatggtgctg tgttatctgc ggctggattc ggattccacg gattatctct ttgtgcagtg
    17581 tgatctggac agtatcgtcc ggcaggcagt gcggcggttt tccgcccggt ttatccaccg
    17641 gaagattcgg ctggaatatg agccgctgga ttgtcaggtg ctgactgatg aaaagtggct
    17701 gctgttcgtg ctggagcagg tgctctccaa tgcgttgaaa tatactccct ccggcgggac
    17761 ggtttcgatt tttctggagg aaccgaaaac gctgtgcatt cgggatacgg gaatcggcat
    17821 tgcggcggag gatctgcccc gtatttttga aaagggcttt acaggctgca acggcagaac
    17881 ggataaaaag gcaagcggca tcgggctgta tctctgccgg cgaatctgcc ggaacctgaa
    17941 ccataccatt acagcggagt cgcaggcaga gcaaggcacc accattcgca tcggcatgtt
    18001 tcgggaaaca cgtggcattg agtgatgtga caaaaatgta agaaagaaag cgggaaatgt
    18061 aagccgaatc gatggcgaag catttcccgt ttgtggtata ctgtagtagt caacaaaacg
    18121 ggcaacacac aaggcttgtg tgtttcccat atacgcaaag atgaaggagg tcattgcaat
    18181 ggcaatgctg gaagtagcac atttgaaaaa ggtttacacc actcgtttcg gcggcaatca
    18241 ggtcgaggct ctgcgggacg tttcctttac ggtggagcag ggagaatata cagcgatcat
    18301 gggggaatcc ggttccggaa aaaccacgct gctgaacatt ctggcggcac tggacaagcc
    18361 taccagcggt atggtgcagc tggacggaaa cgacctgaca cagatcaagg aatcccgtat
    18421 cgccgcattc cggcgggatc atctagggtt tgtctttcag gacttcaacc tgctggacac
    18481 cttttcactg gaggacaaca tctatttgcc gctggtgctg gtgggaacgc cctatgcgga
    18541 gatgcagcgg cggctggaac ctattgcgaa acagctgggc atcacccagc tgctgaaaaa
    18601 attcccctat gaggtttccg gcggacagaa acagcgtgcc gccgtggctc gtgccctgat
    18661 caccaacccg cggctgattc tggcagatga gcctaccggt gcactggact cccgttcctc
    18721 tgatgaactg ctggcactgt tttcggcgat ccaccagtcg ggacagacca ttgtcatggt
    18781 gacccactcc accaaggcgg cggcaagtgc cggacgggta atgtttatca aggacggcga
    18841 agtgttccac cagctgtatc gggggaacga caccaacgaa cagttctatc agcggatttc
    18901 ggacactctg acattgctga ctaccggagg tgaggcacga tgaacatgca tctctatccc
    18961 aaattggcat ggcacggcat tatcaagaac aagaaaacct atgtgccgtt cctgctgacc
    19021 agcatcggac tggtcatgat gttttacatc gtgtcctatc tcacctacaa caaatccgtc
    19081 aaacagatgc gtggcggcgg tgatatgcag ctgatcttgt catggggtgt accggtggtg
    19141 gcattttttg ttgtcatctt tctgttctac atgaattcgt tcctcatgcg tcgccggaaa
    19201 acggagttcg gactgtataa tattctgggc atgggaaagg gcaatattgc ccgcgtgctc
    19261 ctgtggcaga atctcatgct gtttgccgtt tccattgtcg gcggtctggg catgggcatt
    19321 ctcctgtcca agctggcgga actctgtgcg gcgaaaatgc tgtccaatca ggcttctctg
    19381 gcatttgtca ttgaaccttc tgcggtgcga aataccctgt ttctgacagc cctgtctttt
    19441 gtgctgatcc tgctgtattc gctgggacag atccgtgtgg caaagccaat agaactgctc
    19501 cacggggaaa agaccggcga aaagccgccg aaggcacgct ggatactggc actgctgggc
    19561 atgttattgc tgggtaccgc gtattatctg gcgttgacca caaaagatcc catacaggca
    19621 ctgctggtgc tgtttattgc cattgtgctg gtcattatcg cttcttacct gctgttcatc
    19681 tgcggctctg tggcactgtg caagctgctc cagaaaaaca aaaagtacta ctataaactg
    19741 aaccatttcg tttccgtttc ctctatgtcc tatcgcatga agcggaacgg tgccagtctg
    19801 gcttccattt gtatcctctc caccggcgtg ctggtgatga tctcgtcctc tctctgtctg
    19861 cggatcggcg aagatgccag cctgcacacc cgctatccca gagatctgga agtcggcgtt
    19921 ttcactaagg actacgacca gagtgttgcg gcgtcatctt acatcgaaca gcaggtcgat
    19981 gctgtattgc agacatcgca tcagaccaga aagaatgaac tcgcctacaa caatctgaat
    20041 ttcgcggcgt taaggcaggg cagcaatctc tcggtaaaac agtatacgga agtcagcggc
    20101 actcagtatg cggagagcat cgacaaactg gtcatgctga atattgtgac actggacacc
    20161 tacgaaaaaa tcaccggcac aacagaaaca ctgggcgaaa atgaagtgct gctgtgtatg
    20221 tttcgcggaa cgttcagcgg agatgccctg aatatcgatg accagttcca atttcatatc
    20281 aagaagcagg ttcctgactt tcttgaaatc ggtgacaatg ccattgcgac ctatccgacc
    20341 atgtacctgg tggttccgga cggcgaaact atgagccggc tctacgagta ccagtattct
    20401 gtctacggcg aaaaaatggc gtccagttat cagacctggc tgggatttga tctggactgc
    20461 aacgcggaca cacagatgga actggaaaaa caattggagc agcagtttgc cgacgacgca
    20521 cagacacatc aggaatattt ttacgaagtc aaaagtttgg agatgcagcg gtacagcttc
    20581 tactccctgt tcggcgggct gctgtatctg ggcattttgt tgagcgtggt attcctgttc
    20641 ggcacggtgt tgattatgta ctacaagcag atctccgagg gatacgaaga tgcagcacgg
    20701 ttcgagattc tgcaaaaggt gggcatgacc cgaaaggaga tcaaaaagag catcaactcc
    20761 cagatcctga cagtctttgc cgcaccgctg ctggcatcgg gtgtgcacct gtgctttgcg
    20821 ttcccgctgc tccggaagct gctgatgatg ttcgggctgt cgaatacgcc gctgctggtg
    20881 ggcgtgactg ggggcagcta tctggtgttt gcagtgctgt atgcggtcgt gtacaaggca
    20941 acttcccgca gctactacca gctggtaaaa tgatgcggca gcgaatttac cgcggggcag
    21001 tgctggctgt ggggctgatg ctgctggcag tcgcagccgt gccgctggcg gtatttctgg
    21061 gaattgtcgg tgcggtgtgg aaaatcatga actggctgtt ggaaaggggc aatgcagaat
    21121 gaaactgcgg aaaatctggg tgctgccgat gctgctgaca ctggtttgca gcgtgggctg
    21181 cggcgggaaa gcggacctgc tgcacggcta tgaaaaggtg gtgtcctcca tgggctcgct
    21241 ggcacgtacc tcggagcaca agctcattgg cacgcgaacc tgcgggagcg acaactacac
    21301 cggagcctat gccgccacat gtcggcaggc acggggaaaa gatgtggttt tcggcgggtg
    21361 cagcttgcag gcaaggcggc tgcaagtcac gggctcggtt gcctgccagt ccggctttgt
    21421 gcagctgatt gtgaaaaacg gcagcgacga actgtggctc acacccgatg caaacgggaa
    21481 catctcacag gagatcaccg gcagcggcgg cgactggtat ctttcggtgg aatatgccga
    21541 tttttccggc agcgtgacat tatgcagtgc ttatgcgaaa tgaaaaaaag cgtatcgtct
    21601 gggacggtac gctttttgct atttcacacg gacgatcgtg tttctttttg ctgagcctgt
    21661 tgttcggaga gggtgtcgag aaactgctcc agcctgccct tgttgcgttc ggtgagggcg
    21721 gtgtattttt gcagcaccag cagggcatcg tctgcaatgg tgatcccgtg aggatttttc
    21781 gggagattgg tgcgtccggc gagatagtcc agactgacat cgtagaaatt cgccagcagg
    21841 acggcacggg aaaagggaag ttctcgttct cccttttcat atttgccaca atactgggca
    21901 gttgttccca gatatgctgc aatttcagtt tgtgttttgt ctgcatcttc ccgcaagtcg
    21961 cggattcgct gataaaaagc catttgtgcc actccttttt gcatttattt tatcataaat
    22021 gactaaaaga tattgacttt tcaatctaaa tggattataa tagataaaaa gaagtcagat
    22081 gtgactactt taaaattgaa ggagttggta caaatgaaaa catgcagaag aaaatggctt
    22141 gctcggtggc tcgcggcttg tattgcggtc gtgctgggca tgggaatgtg tgcggggctg
    22201 ccggtgagtg cggcatatga gaacacctat accaataccg gaaatcagcg tgccgatatt
    22261 gtggcagttg cgaaaacaca aatcgggtat catgagggca gcctggaggg aaccacaaac
    22321 agcagcaaca attacacaaa gtacaacgtc tggaacggga aaattgaagg cggttatcgc
    22381 tatgcgtggt gtcatgcgtt tgtttcttgg tgtgccaatc aggcagggat cggaacggat
    22441 attgttccga aaacagcggg aacaagcact ggcagatcat tttttgtgaa tcagggaact
    22501 taccggcaga gtgcggcaaa cggcggaagt tatgtgccgc aggctgggga cattatttat
    22561 tatggctccg gttcgtcgcc gtcccatgtg ggaattgttt ctgattgcga cgggagcaca
    22621 gtttatacga tcgagggaaa ctattccaat aaggtaggga caagagcaat caatttgtcc
    22681 aatagctata ttattgggta tggtgtgccg aattataagg gggtgaaccc gaatccgccg
    22741 aagtgggcaa aagtaacatt agaccactat gcctggatgg caggggaaac actgacattt
    22801 aacctgtcca gcgaaggcgg tgtggcttat acagtgggta ttgatacaga aaatgctgac
    22861 ggcggaagaa ctcgaattga cacacatgac accagagata cttcttattc gagatcattc
    22921 aatagtccgg gaaagtactc ttgttatatt accacctata atgagtatgg actggcggac
    22981 agtgaaagaa tctattttga agtatacgac aaaaagccgg aaaatgtaag tgtatctctg
    23041 gatcactacg gctggatgtt aaacgaaaat atgaccttta cttgttccag tgtacatggc
    23101 tatgcttata caattggtat agatgatgca aacggaaaca gaatagatac ctatgataca
    23161 acaggaaaca catatacaag gtcgtttagt acacctggaa aatattcctg ttacattacc
    23221 acatacaacg gatatggata tgcggacagt gaaagaatct attttgaagt atacgacaaa
    23281 aagccggaaa atgtaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctgg
    23401 aaaatattcc tgttacatta ccacatacaa cggatatgga tatgcggaca gcgagagaat
    23461 ctattttgag gtgtatgacg gaaagccaac gaatgcaaag gtagaattgg ataaagattc
    23521 ttaccatgta ggggaagaac ttgtgttccg ttgttccagt gagcatggct atgtgtatac
    23581 aattggtata gacagcgaag atggagaacg aacgcggata gatacctatg acacaaaaga
    23641 aagttcatat acaagaagct tttcgaaacc cggaaagtat tcttgttata tcacaacgta
    23701 taatgggtat ggactagcag atagtgaaag aatctatttc acaatactgc cgtcacccgg
    23761 cgacctcaac tccgacaaca ccctcaccac cgccgatgcc gttctactcc agcgatacct
    23821 cctacaggaa cgcaccctga cacaaaccca gtggcagacc gctgacctca acgcagacgg
    23881 cactgtcaac ggcttcgact tggcactgct ccggcagaag ctggtggcgt gagtttctta
    23941 gaaagtccct cttgaaaagc atgaaagttg atttttagaa gaattacaga taacccctca
    24001 gtcagcctta cggctgccag ctcccctttc aggggagccg aataagcctc ctctgaaagg
    24061 ggagggggac cagcgaagct ggtggagggg tattccccct caagtatcca atagaattga
    24121 ttttcgtgcc gatataccca gcccaacaga aaagccgtac cccggaaatg ttccacttcg
    24181 gggtacggct tctttgatac gattttgatt ttgttttagc ggatcttcga acctaccggc
    24241 aggctttcgt cagggaagat caccttgaca tcaccgttgg gcatatctgc cgcacagatc
    24301 atgccgcagc tttccacgcc gcacagcttt gccggcttca ggtttgccac cagaacgatc
    24361 ttcttgccga tcaggtcttc cggcttgtac cacggtgcaa tgcccgaaac cacctgtctg
    24421 ccgcccatgc cgtcgtccag ctggagcttc agcagcttct tggacttctt taccttttcg
    24481 cattccagca cctttgccac acgcagatcg gtcttgccaa aggtgtcgat gtcgatctcc
    24541 tctgccagcg gctggggtgt gaattccggc tcctgcggtt ctttcttggg ctgtgctgcc
    24601 agaatcagct ggttcagttc gtcgatctcc ttttccacgt cgatacgcgg gaagatgatg
    24661 tcgcccttgt gcacggtcac atttgccggc agtgtgccga ataccgcagc ggtgctgtat
    24721 gccacgtcgg cttccgatgc cccgatctgc tcccagatct tcggcatggt ttccggcatg
    24781 aatgcactca gcggaacggt gatgatacga atggtttcca gcaggttgta cagcaccgtt
    24841 gccagacggg tgcggtttgc ttcctctctt gccagaatcc acggtgcggt ttcgtcgatg
    24901 tacttgttgg cacgggaaac cacctggaaa atctctgcca gcatgtggtt cagctgcgtc
    24961 tgatccagca gaccttccag ctttgcagtc agaccggtgg ctgcggcgat gagttcctcg
    25021 tcaaattcgc cggactggcg ttcggtcggc agagtgccgc cgaagtactt gtccaccatt
    25081 gccacggtac gggaaaccag attgcccaga ccgtttgcca gatcggagtt gatgcggttg
    25141 atcatgatct cgttggagaa cgaactgtct gcacccagag ccatttcccg caggatatgg
    25201 tagcggatcg catctgctcc gtagcggtca gccagcagga acgggtctac cacgttgccc
    25261 agcgacttgg acattttctg tccgttgaaa gtgatccagc cgtgaactgc cagatgcttc
    25321 ggcagcggca gatccagagc catcagcatt gccggccagg taatggcatg gaaccgcatg
    25381 atgtctttgg caaccatgtg cacatctgcc ggccagtact tgtcgaaatc gtggtaggcg
    25441 ttgttttcat agcccagtgc actgatatag ttggacagag cgtctaccca gacatagacc
    25501 acgtgcttcg gatcgaaagt gaccggaatg ccccatgtga aagaggtgcg ggaaacgcag
    25561 aggtcttcca gccccggctt gatgaagttg ttgaccagtt ccactgcacg ggtcttgggc
    25621 tggagatagt cggtttccag cagcaacttt ttgatacgct ctgcaaaggg agccattttc
    25681 atgaagtagg cttcttcctt tgcctcgacg acttcgcggt gacactccgg acagcagccg
    25741 ttttcgtcca gctgggactc ggtccagaag ctttcacagg gggtgcagta cttgccctta
    25801 tattcaccct tatagatgta tcccagatcg tacagacgct tgaagatctt ctgtacggtt
    25861 tctatgtggt agtcatcggt ggtgcggata tagcggtcat agctgacgtg cattttttcc
    25921 cacagtgcct tgaaggtctt gacgatctca tctacatatt ctttcggcgt gatacccttt
    25981 gcagccgctt tctgttcgat tttcagaccg tgttcatcag ttccggtgag gaagatcaca
    26041 tcatagccct gcattcgtct gtaccgtgcg atagaatcgc aggcaactgt tgtatagcag
    26101 tgcccgatat ggggatttcc cgacggatag taaatcggtg tcgtgatata atacggcttt
    26161 tttgacatac aaggtcctcc ttaacattta tgtgcccgaa ggctctactt cttattatag
    26221 cacagttttc cggtgttgtc catgtttttt tgaaaatttt gtaggggatg gggtgcggaa
    26281 cttgcttttt tggagaaaac atgttaaaat aatggaaagc gtaatgctac gatacttttg
    26341 aatatgggcg tagatctcaa aatcatcagt gaccatttag ggcattgcga catcaatgtc
    26401 acagctgata tttatgctga tgtgcttagc agtacacgaa aagtgttggc agattctatt
    26461 gagaagaagc tgactgaata aaaatacgag ttacagcaac ataaaaaaat cgctctgcaa
    26521 aacacagagc gattttaaaa tactttttag ttgtccagaa gttgtccaaa tagacttctg
    26581 aagcaaaaga aataacgcaa tatcgataaa aaatcgatgt ttccttatct ctttgagaaa
    26641 caaaaatatc ggattttagg ggactttttg caccttgtta gttatgtgtt agttgttgag
    26701 ttgttcgtat aaaggtggaa cacatcaaaa atttttgagc aggcattgtg agcggctggt
    26761 gtcatgatgc ttttttctgc atagaatcga ttccagcgtg aaatcgtaag attgactgct
    26821 ggggaaatgc ttgacttttt ctatttctta tgctatattt gtgaaagaaa cgaaagaaag
    26881 taaaagaaag agaaagaaag ggggtatatg cttttggacg acagcagtac taaattcaag
    26941 agaatctatg taaataaggc aaacttacga aatatgtggt ggactagagg gaacattgca
    27001 caggctgaat tacttgcgga ggaagacttt atataacaaa acaagcacgc caaaaccata
    27061 aatagtgggt tcttggcgtg ctgttttatg agagaatggg attccaaata cgctatattt
    27121 caatatgctt ctgaatgagt ttgcaatgcg actttggaca tcatttcctg gaattttttt
    27181 tgttttttag cattcccttg aatgcacctt tcggcgagcc atagagatgc agaaaagcga
    27241 tcatctgctt gtcttagctt tccgatcatg aacgggaaca gcttgctaaa cgtccagaaa
    27301 attgattcac tggcacattt gattaccagt tcctcgctat aaaccttatt ttttacagca
    27361 actgcaaaaa attcgaattt agagagcatg attttatatt ctttttcttg tagatctgat
    27421 ttgatttcat agagttcagg acaatatatt ttgctcagga cctcatcttg cagcttgttt
    27481 agtgcatcta ttgtcaggcg agttcgttca ttatcttgct catgatcgga tatatttctg
    27541 gaaacgtcaa aagctttata cgctaaacag actgccacta gacttcctac tgctgaagca
    27601 gtatcaacaa ttgcttgata tatttctaaa gacatacaac atcctcctta ttctccataa
    27661 acctcaaaat accacaaaag gacaacctcg aattgaaaaa acgaacttgt ccttctatca
    27721 taatgattca tctgtctaac atggcataag tttgtaaaat ataggcttac ttcacgtatc
    27781 ccacatactc accgtaatgc cagcctgcta catcgtaagt caaacctgca ccagtagctg
    27841 cggcgtagtt cagaagttgc tttacatcag aataatttac atcgtcgcag ttcatgattc
    27901 tgccatcctt gtttccatct gctgcctcta ctttttttgc ggagtccagt ttatattttg
    27961 ccggattcat ctttgcaata gaaaccatca gagcttttcc gtttgctgtt gtcagcaggc
    28021 tgccgtcagc ctcggcgata ttgtatgcag acagcatgat cgcagcatcc ttggagttga
    28081 cctgttcatc gctgttcaaa tcgccaatta cgtaaaaatc atactgctga ctggtatagc
    28141 agctgatctc attgttcttt acagattcga acgaggcatc ttctacggtt acttctgcgt
    28201 cactggtagt atagaaggtt accagagtgc cgtcatcctt ggaatcgtcc agagatgcac
    28261 tggtgataac aaccttgctg ccgttctgtg cagcagatac gatagcatca ccataagcac
    28321 tgccgtttgt tgcggcaacc ataccgtcca cagccaacag gtctgcggtt gcgttcagcg
    28381 tgatgtcaga tgctgagaaa ccggtgttgt tctcgatgga cagtgtcact gcggttgcac
    28441 cagccggaac aactgtgccg tcagcggcgg tgaatgtttc tgctacggtg ctgtaggaaa
    28501 cattgactgc gtttactgcc tcagctgcgc ttgcaacaac gcctgcgcaa gcagatacca
    28561 ttgtggctgc agataaagca gcaataattt tttttacaga cttcataagt agtacctccc
    28621 tttcggatat gtaatgattt ttaagttgtc tcctttcttt tgctctcctg ataaatcagc
    28681 aaagcaaaag tcactttttc attttcaaga acagaactat ctctattttt tcagtcctgc
    28741 tccacgcag
//
LOCUS       DAWBGR010000009        24961 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_745_length_24961_cov_4.603509, whole genome shotgun sequence.
ACCESSION   DAWBGR010000009 DAWBGR010000000
VERSION     DAWBGR010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 24961)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24961)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24961
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_745_length_24961_cov_4.603509"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5546 a         6914 c         5997 g         6504 t
ORIGIN      
        1 cataagtgga aacccctcag tcagtgctac gcactgccag ctcccctttc aggggggctt
       61 acttgtcaag cttcacaatt ttgcacaatt taaatcaaca tatttttgaa aattgatttc
      121 cgtgcaccgt attcgaaaaa acggcataca aaaagcggac aggtcgcccc gtccgcttcg
      181 tttcttgata catcattttg ctgatgcgtt tattcatagt cgaatacgtc gatccaacgc
      241 atgaggaagt ccttttcctc cggcttgtgc ttcaccagct gcgatgctgc cacaatcggc
      301 atccactggg aaacgtactg tcttgccacg tcgttcatgt cgcagaacat tttcaggtac
      361 agctctgccc agtcgtcctt cccctccagc ttgaacagca ggaatgttct ggcagcgtca
      421 gcactgccat tgccctgtgt ggcgtgggac cagtcgataa cgtgcagtct gccgtccttg
      481 tccacgatca cgttgctggg gtgaaaatcc ccgtggcaaa ccttgatgtg gtcatgcatg
      541 gcattcagac gggtatgcag gtcatagcgg gtggtcgcgt ccagtccgga ctggctgatc
      601 ttccggttca tcttgtcttt cagcttcgtc agcagcggcg aacgcttgct ctggatctcc
      661 ttttgcagca gcacgaactg ttccatatag ttgtgcatct tgtcggggtg ctgttccatc
      721 atggactgga gggtttcccc ttccacgaat tccgtcacga tcgcccactt gccgtctgct
      781 ttccgcactt ccagcagctt cgggatccgc agaccggttt cttctactct tgcctgattc
      841 agtgcttcat tcagaatgtc ggacttcaga tagccgtgtc cgaacacctt tacgcgggta
      901 tcgccttccc gatagctggt tttctccagc ccttccaata acggttccat cattgatacg
      961 ctcctttcac agtggaatta tgcttcagca gcttctgccg gcacttcgcc gtagaacgcc
     1021 ttgagataca ttgccttgat ctccgagatc agcggataac ggggatttgc tccggtgcac
     1081 tggtcatcga atgcctgttc gcacatgtcg tccagtgtcg cgaggaatgc ttcttcggtc
     1141 acgccgtagt cgcgaatggt ttttttgatg cccacccgtg ctttcagttc ctcaatcttc
     1201 acaaggaagt tttcgaatac ttcttcgtcc gtgctgccgt tcacgcccac gaatcttgca
     1261 cactcgcaat agcggggcag agcgttcggg tacggatact gggagaaagt gcccattttc
     1321 tggggagcat ctgcaatgtt ataccgcata atatcggtga tcagcagagc atttgccacg
     1381 ccatgaggca ggtggtggaa tgcacccagc ttatgtgcca tagagtggca cacacccaga
     1441 aaggcattgg caaacgccat acctgccatg gtggaagcat tcgccatctt ttcccgtgcc
     1501 accggtgcat gttcgccatt ttcgtagcac tccggcagat aggtgaagat gttcttcagt
     1561 gctttcagtg ccagaccgtc ggtgaaatcg gttgccatta ccgaggcata tgcttccagt
     1621 gcgtgtacca tggcatcgat accggaagcc gcagtcaggc cacggggctg ggacatcatc
     1681 aggtcagtgt caatgatcgc catgttcggc agcagctgat agtctgccag cggatacttg
     1741 gcaccggtcg cctcatcggt aatcaccgca aacggtgtca cttccgaacc agtaccggag
     1801 gaagtcggca ctgcaatgaa atatgccttt tcgcccattt cagggaactc ataggttctc
     1861 ttgcgaatgt cgagaaaccg cattgccatg tctgcaaagt ctgcatcggg atgttcatac
     1921 agcacccaca tgatctttgc tgcgtccatt gcggagccgc cgcccagtgc aatgatcacg
     1981 tccggctgga acgaccgcat cagttccgca ccctccttgg cacacgccag tgtgggatcg
     2041 gactgtacat cagagaatac ctgatacgga atgcccattt tctgcagcgg ttccgtaatg
     2101 cagctcatct tgccgctttt gaacaggaat tcatctgtta ccagaaatgc acgcttccgg
     2161 tgcaggactt cttcgatctc acgcagagcc accggcaggc agcctttttt gaagtacacc
     2221 ttttgcggtg tgcggaacca cagcatattt tctctcctct cagcgacagt cttgatattc
     2281 agcaggtgct tgacaccgac attttccgaa accgagttgc cgccccaaga gccgcagccc
     2341 agcgtcaggg acggtgcaag gttaaagttg tacaagtcgc cgataccgcc gtgagaggac
     2401 ggggtattga tcacgatacg gcacgctttc atacgtgctg caaactggtc gatcttttct
     2461 ctgtggttga cttcgtccac atagagcgaa gctgtatgac cgtagccgcc gtccctgatc
     2521 agctggtcgg catatgctac agcctgttca aaattctccg agcgatacat tgccagcacc
     2581 ggagagagtt tttcatgtgc aaattcttcg ctgatgtcca ccgaggtcac ttccccgatc
     2641 agcacccgcg tttcctgcgg gatcgtcacg ccggcgagtc cggcaatggc agccgcccgc
     2701 tgtcctacga tcttgctgtt cagtgctccg ttgatgagaa tggtctttct taccttttcg
     2761 gtttcctccg gtgtgaggaa atagcagcca cgcttctgga attctttccg gacagtgtca
     2821 tagatgccgg tatccacgat gaccgactgc tccgaagcac agatcatgcc gttgtcgaat
     2881 gtctttgagt gtacgatcga gttgactgct ttctgaatat ccgcagaagc gtcaatgact
     2941 gccggtgtgt tgccggcacc aacgcccagt gccggttttc cgctggaata cgccgcacga
     3001 accatgcccg gaccgccggt agccagaata atatctgcct cctgcatcag ggtattggtc
     3061 atttccaaag acggcacatc gatccagcca atgatatttt ccggtgcacc agccttgact
     3121 gctgcgtcca gtacgatctt tgccgcagcg atcgtgctct tttttgcacg ggggtggggc
     3181 gaaatgatga tcgcatttct ggttttcagg cagatcagcg acttaaaaat cgcggtagag
     3241 gtggggttgg tggtgggaat gaccgcagcc actaccccga tcggttctgc gatccgcatg
     3301 gtgccataag cgggatcctg ttcgataatg ccacaggttt tggtgtgctt ataggcgttg
     3361 tagatatact cggcggcata gttgttcttg atcaccttgt cttctaccac acccataccg
     3421 gtttcttcca cagccatttt tgccagcggc agtctggcgt gattggctgc cagagccgct
     3481 gcctggaaga tccagtctac ctgttcctgc gagaacgttg caaactgttc ctgtgcacga
     3541 cgcacttcct ccagcttgcg ttccaacgcc ggaacgccat ccactaacgg tcgattgttt
     3601 tctgtttgat tcattcaaat actccttttt tgggaaacgc cgaaacagtc cggtgctcct
     3661 cagggtagaa gaatcccctg aggcggctgt ttgtatccaa agatacgaaa caagtcctga
     3721 ggcaataccg cacaaagatt gtgcggtatt gccctgagat ttccaaagcg tgagagattt
     3781 gtggaaagta cagcagcatt tcttatgcgg actgtaccgg tcggttccat tgaatcatgg
     3841 acggtttttc ttctgtgaca acgatgtcca tagatcccca tctgtgacac ctttgtcact
     3901 gctattatag catatttccc gtcctctcgt caagccacag attgttcacc gatttccgcc
     3961 cgcagccgtc ccctattccc gacaggtatc gcatggaaaa catgctatac tcttgaaaga
     4021 ggtgatgaac agtgcccacg atctatctgg acgttctgct gctgcagagc ctctatgtca
     4081 actatttttt gctgcgtgct gcggcaaaac tgacgcatac gcctttgcac tggatacggt
     4141 gcggactggc agcggcgggg agcagcctgt tttccctgtt gatcctgctg ccgcccttgc
     4201 cggcatggtt tcagctggtg tgtaagcttc tggcagctgc tctgtccgtg acaatggcgt
     4261 tcggtctgca tcgggagagt ttcttccgcc agtggggctg cttttttctc tgcaactttc
     4321 tgctggcggg ggcgattctc ggcatcggca gtctgacaca aaagggcttt gccacatggg
     4381 ggaacagctg ctgttatctg gatttttccc tgtttcagct gattctattc actgccgcgg
     4441 catattctct gctgcatctc tgcaccctgc tccgcggcag gaaacgccac accgacagcc
     4501 ggtttcaggt ctttctgcgg ctgggcgaac gccgcttgat gctggacgga cttgccgaca
     4561 ccggaaacaa cctcaccgac accttttccg gtacgcctgt cattgtgtgc agtgctgccg
     4621 cactggagcc gctgcttgcc ggcacaccgg tggaacagct tcgcggctac cggctggtgc
     4681 cctgtaccac agtgaccgca gagggactgg ttcccctgtt ccgaccggac gaggtctgta
     4741 tccgcaacct ctccacggag aaaagccgca gcgtacacgc catggtcggc attgccggca
     4801 cgcagccaag tgccattttt caccctatgc tcatggaatg aaaggagatt ttttctatga
     4861 ccatcgattt tccgaagttc cggaaactgc tctgccattt gcgggaactg ctgtccggtc
     4921 cctgccgcat cgactacatc aacggctctg acacctttcc cccgccgctg tccaaggagg
     4981 aggaacagga agtgttcgcc cagctgcatg ccggcagcac cgaagcccgc gaaacactga
     5041 tcgtgcacaa tctccgcctg gtggtataca tcgccaaaaa gttcgaatca tccggtctgg
     5101 gactggagga tctgatctcc attggcacga tcgggctcat caaggcggtg gaaacatttt
     5161 gccccgaaaa aaagatcaag cttgcgacat acgcctcccg ctgcattgaa aatgagatcc
     5221 tcatggtgct gcggaaaaac gcccagctgc ggggagagat ctccattgac gagccacttt
     5281 ccgcggactg ggacggcaac gaactgctgc tgtcagatat tctgggaacg gacgaggacg
     5341 tggtcagccg cgacattgaa aacgaagccg aacgggagat gcttctcgca gcggtttcag
     5401 aactgggacc gcgggaacag gagatcatgc agatgcggtt cgggctctgc ggcggcaagg
     5461 aacgcactca gaaagaggtc gccgaagcca ttggcatttc ccagtcctac atctcccgac
     5521 tggaaaagaa gatcattcgc aagctccgaa aaaaactgga ggaggcagac cagcccctca
     5581 cccacaccgc cagcaaataa caagagccgc agggcatttc gctctgcggc tcttttgagt
     5641 tatgataaaa tgattagaaa attgctcaag tgtgcattag tctgcatacg gcagctgctt
     5701 taccagagat accaggaact tcagcaaagc gactgcatcg ttggaagcga tttcgccatc
     5761 gccgtcacag tctgcatttg ccttctgctg acgggtgccc agtgttacaa caccggcaat
     5821 tgccttgttc agcataactg catcggtaat gtctacacgg ctgtcgcagt tcacatcgcc
     5881 atacagcgga atgatcactt cttctgtcgt agtggtggtt tctgtagaac caacggtagt
     5941 agttattttt tctgttgtag tagtcgttgc actggttgta gtagtagtgg ttgtttcagt
     6001 tgttgtaaca gtagtctgct gcggcagcac gtttacagta acttccttcg tctgaccgcc
     6061 cggtgtggta actgtcagcg tgctagagcc aacattctta ccagtgataa ccagcttcag
     6121 agcgtcgtcc ttgtctgctg ctacacctgc aacggttctg tctgccatgg tgccggtgca
     6181 gttcggaatg tttactgtca gagtttcgct ttcgccgatc acaacatcga tagaatcctg
     6241 tacttcgaga gtatcctcga acatatccag cttcttctcg ttgttcttgt cgtctgctgc
     6301 atagaaaacg gttacgttgt taatggtgta cttgctggat tccttgctgc ccagacctgc
     6361 ataccagaca ccgaagctca gatgtgcatc ttccgattcg gtatccactg aattcagtgc
     6421 attcagcagg ttgccggttt cttcctgacg gattccctca gcaacagacg gcggcatgat
     6481 ccacatataa gtataagtct ttgtgccaga agcgtctttg tacgcctcat ctgccaatgt
     6541 gtcatcgtta gagtccggac gattgcctgc ggtgctcttt tcccaataca gcattgccgg
     6601 agttgtgctg ttcagacctt cattcttaaa caggtcatca gactggaacc agttgtcagg
     6661 aaatccagcc ttttccagca tcgtggtacc tgcaccaacc agaacctgat ttgcttcctg
     6721 atcggtagtt acatcgaact gtactgcata tacgtcagca gtcttttcat acttcatgcc
     6781 gaaatctgca tacgggatct ccgcagctgt accggtcatt tcagtacctg cgttcttcac
     6841 agaagcagaa ccgctgtacg ggaatgtaat gctttcttca taagtcaggg ctgcggaaac
     6901 aatgctgcag tccacaaact tgccgccctt cagagctgca taccacagct ggaaggaaat
     6961 ctggctcttt tctgccagtg tttcgcagtc aacgacctgt tccggaacgt cccacgtaac
     7021 tgtgaagtag ctgcccatct cctgttttgc cagatcagtg ccgccgccaa ctttgaagtc
     7081 gccttcgccg ttgccccact ttacgccggc ttcggtgcat tctgccagtg tttctgcacc
     7141 aacatcattg taccaataac ctaccgtctt ggctacgccg ccttcgccgg aaccgccctt
     7201 cagaccagcc tgctccttcg catactctgt atctgcaagg ctcttatact tcacgttcag
     7261 accgccgcca tacataaagc ggtttacatt tgcactttca tcgtccagct tgatggtaac
     7321 gctcagggac tttacctttg cattgctggc aacggttgca cccggaatga aattgaattc
     7381 accataagta aacttgtggc tgttcagcgg cagaccagct tcacgaatct gcgattcaga
     7441 ttcataagcc acaccgttgt tcttttcttt gaatgcatca gaagcataat attcttcgct
     7501 gcagttgtca cccggtgtca gcttgatgtc cagattatcc agcttcaggg agtgcagcga
     7561 cttaacagtc atagtgtcac cgttctgctc tacggaactg atctcaccgt tgccgtttgc
     7621 actggagtag tacagaccgc cggtgttttc ctcgcccttc ggcagagtcg ggtcaacgac
     7681 cttgatccac cacggctttt caccgttgct ggtatcttcc gtaccgttta ccgtaatgga
     7741 aaccaattca atgctggcat cctgtgtacg ggtggactgg tcttcttcgt caatcagtcc
     7801 ggtatagcag ttctggaact ggaactttgc attcttattg ctagtcagct ccttggctac
     7861 actggacggc agttctacaa ctacagaaaa ttccttggca tacggtgtgc acttgatagc
     7921 acccttggaa tccgttttct caaagcccca ccagtagccg tttgccttgg tgtccgatgc
     7981 accaaagtag taggagaaat ctgtgaccat ctgatcagac ttcagattaa actgcaaggt
     8041 ggtgatgctg tcgccgcaaa tatccgctgg cgacttgccc ttggtatctg taaacgaagt
     8101 atacgcatca gttgcagcct tgcacatata tgtgcacttg tctgcgttca gcggattgtc
     8161 cttgtcctcc ggattcggaa cggaggtttc cagtgtcttt ccgatcgtta cagtacttcc
     8221 tgctgcttca acaaaaaagc tgccggaagt aactgtaaca gaacttgcca ccataagtgc
     8281 actgacaagc agtgcactga ctcttttgcg aatgatcttg ctcatatata tcgctccttt
     8341 ttcttttaat tggttttcat cagtcccaat ttcttttgta tgcacgaatg tcagctgata
     8401 tgcaatcagc tgtcttattg tccgccgtgg acggcagact gtccgcggcg tttacgcttc
     8461 aaaaatgaca cattcatttt ttagctatac actattataa caactttccc ctcgaatgta
     8521 aatgtataaa ctattaacat tcgaacttgg caggagaatt tatgtgtttt gttttgtgca
     8581 tgttgcataa aagtatttca catagaaaca aaaatacgac tttaaataaa aaaagaaaag
     8641 gcagctcttt gaaacgccgc cttttcattt atactttatc caatctttcc cttgttaatg
     8701 attgctattt tccgacagat ctgactgcgt ttgttcggaa ttcacagtcg gattgcccga
     8761 tgtccgcacg ccctgcactg caacacgata atcaccagtt gcccatacgg tattgtactt
     8821 gctgcaagtg atcgtaggcg tataattaaa ggtcaccgtg tcaccgtcta cggctcccgc
     8881 ctgcgtggag taactcagca gatccacgtt ggcggtgagg tcgctggcat ccagcgtttc
     8941 catcacatca gccggaccga taatgctgac tttcagctgc tgggtcagca cattgaaatt
     9001 atacgttgcc ggtgcgttga ctacgccaat atccgagatg acaaaatcct ttttcacaag
     9061 atctgtggaa tccagtgcca gtgtcacggt agagaatccg gactggttca catagtcgtc
     9121 cggcacattg actacgaagt cattggagta gttcagatca atatcccgca gagccactga
     9181 accaatattg aactcgctca tttcctccat agaggtattg ggtgctgcga gggtgatctg
     9241 ctgttctgac aggttcagcc gctttttcag agcttccaga tcgaagtttg ccggtgcatt
     9301 ggtgatctga taggtgatgt ccatggtttt ctgatacagt accggcactt ccagcgagaa
     9361 cgccgccaca tcataggtaa aatctgtgga gtctacctgt gtgcctgctt cattatagaa
     9421 gagcagcgta tctgtggtca gaattttgga ggcactcagc tgctcctttt gctgggtttc
     9481 tgccacgcag tatgccactt gattcagctg ctgctgggga ccggtgacat caatggtaga
     9541 cggtgtgcag gcgaagttct cctcgtccag cacacagccg tctgcaaatg tcagattcgg
     9601 agcagaagcc ttgatctcaa aggtataggt ttcgatttta tcaaaggtca cattcactgt
     9661 agcgggcgaa atggacttga cattgaactg ttcgttttca ttgccgctga ccgtaatgga
     9721 aagctttttc gtgccggcag aggtgacatt ttccaccaca gccgttgccg tcagattgtt
     9781 cgcatcgatg ttgcccacct tggagcgatt gccctcgatc tggaccgtca cgtcctgtac
     9841 gtcatagtcg atgacgctga gtccgttttc ctcggcagaa gtgccggcaa tttccacttt
     9901 cagcggcaca tggtgcaagg tacggggtgt ggtcggataa atggcaatgg agatcacaaa
     9961 ccagagaaca atggcaatcg ccaccgaagt tgccatcatt gccggacggc tctcatacag
    10021 ccgctggaac aatgtcccat tagtgccgct gctccgattc ttctgtgatg tctttttctg
    10081 tcggttcttc cagagatttt tgggcattgt ccttctcctt tccgtcgtcc ttgtggtgcc
    10141 gtttgaggaa cgggatcttc cgtgccacct ttccgatcgt ggtttcctcg ctggcggtaa
    10201 tcagccgctt ggtcagaagc tggtggaggg attcccgcgt aaagccgctt ttcagcacgc
    10261 cgttttccgc cacggaaatg gcaccggttt cctctgaaac cacgatcaca acggcatcag
    10321 agttttcgct catgccgatc gccgcacggt ggcgggaacc cagccgctta ttgataagtt
    10381 cctctttctg aggcttgggg agaaagcatg ctgccgccag aatcttgccg tcgcggataa
    10441 tcaccgcacc gtcatgcagc ggcgttttgt tgaaaaagat attcccgaat aattccttgc
    10501 tgggaatcgc atcgagtatc gtgccgttgt cgatctgttc tcccagacgt gtctgccgtt
    10561 caatgacgat cagtgctccg gtcgaagtcg ccgagagttc tacacaggca tcgcaaatca
    10621 catcaatggt gcggctccac acgctgctgt ccacgttgga gctgccgctg aaccgcagcc
    10681 ccagtttggt ataccctgcc ttttccagcg ttcgccgcag ctccggctgg aacaaaatga
    10741 tcagagcgat caagccaata ttcagcgttt cggaaagaag ataggaaatg gctttcatgt
    10801 tgatccacga agcgataatg tacacgagaa gcaacagaaa aatacccttg atcagctgac
    10861 cagcacgtgt ttcccgtatc agtttcattc cgagatatac cacataggag agtaccagta
    10921 tgtccagtat atccaccggc tgtgctgttt tgatgacgct gaaaatcata tccagtatat
    10981 cccgtaaagc catgtgcatc atcttccttt ccgacttctg tgggaacagg gaacagagcc
    11041 aacaggcgtg ctctgtttcc tgctttcctg tattttgtca ttggcaattc gtctatgtag
    11101 cagacaaaag gcatgctgtg cctcctgtct gccgattttc tgttcaaaac atatcccata
    11161 tacctattgt aacatatcac cggaattttt caataggttt tcgggacttt tcagaatatt
    11221 gacataattt ttcaggtttg ccgccagccg cagcgtatct gatgccctgc tgaacaccga
    11281 cggagcgaac cgaactgttc cgtccttggt gcccagcttt tcgtgagcca gcggtgcaca
    11341 gtgcagtccg gcacgcagac agaacccctg ccggttcagg tagtctgcgg tttcttccgg
    11401 cgaagcccct gccaccgtaa aggaaacaat gggcacatat gccacgtccg gctgccggta
    11461 gaccgtgatc tccggtatct ccgccagtgc atcgcagagc agctggcaga ggtggctctc
    11521 gtgacggtac aagcctgcca ccgtctgccg ccgcacaaag tcgatgccgg cacagaggga
    11581 cgcagctccg atcacgttca gcgtgccgca ttccagacga tccggcaaaa agtccggctg
    11641 ctccggactg cgggaagcac tgcctgtgcc gccctgcatc agcggctgta tgggaaatgt
    11701 gcagtctgtc atgaggattc ccgtgccggt gctgccgtac agccccttgt gaccggcggt
    11761 acacaggata tgcatgccgt cccgttccaa tgacaggggc aggatcccgc atgcctgtgc
    11821 cccgtctgcg atcatacaga tgttccggct gcggcagaac tgggcgattt cccgataggg
    11881 caggatctgc cccgtcacgt tgctggcaat ggtgcagacc accgccttgg tatttggtcg
    11941 aaccaatccc gccagccgcc gcagcgtttc gctgttgtcg gcagaaacct ccagcatgct
    12001 gtaggtgacc tttcctgccc gtgccagtgc cataatggga cggtagaccg agttgtgttc
    12061 cagatcgctg aagatcacat ggtcgccgga tttgagaatg ccctgaattg ccacgttcag
    12121 agcgtgggtg caattcaggg taaaaatcgt attctctacc ttggcatcaa aaaattccgc
    12181 cacggtttct ctggcgtgat agaccagttc cgacgtgcgt tctgcgatca catgcccgcc
    12241 gcgtccggca tttccatagc gtaccactgc atctgccacc gcacggcgaa cctccggcgg
    12301 cttgggatac gtggtcgctg cattatcaaa attgactgtc accggacatc acgcccctct
    12361 tgaggactcc ggaacgggat cccctctctg gacagaagtg cggatacacg gccgcagtct
    12421 gcattcgcgt tcagaacaaa gccgcagccc tctggcccca gatgctccag ccggtagatc
    12481 gcactggaga tgccatgctg ctgcaacacc cgttttgccc gatttgcctg cgtatacgaa
    12541 accattgccg ccttacattc caacatcttc tcaccccttt gcaataaaat cggggaaaca
    12601 cttccccgtt tcagtatatg caaagggaca gcaatggggt gacaggagtt atggcaatgt
    12661 ttcctttatg cctttaccag caggcacaca caatgagcag aaatgcctag tccttcgccg
    12721 gtaaagccca gcttttcttc cgtggtcgct tttacactga cctgctctgc ggatacgccg
    12781 catgctgctg cgatcttttc ccgcatggag ccgatataag gggcaagctt cggcttctgg
    12841 cacagcaccg ttgcgtccag attgcctact gcatagcctt gttcccgcac tttttccacc
    12901 accagttcca gcagcttcag actgtcagca ccggcatagg caggatctgt atcggggaaa
    12961 tgcttgccga tgtcccccag tgccaacgcc cccagcagag catcggaaac cgcatggagc
    13021 agcacgtctg catcagagtg ccccagcagt cccttttcat aggggatctc tacgccgccc
    13081 agaatcagct ttctgttttc cacaaggcgg tgcacgtcat agccgtgccc gatccgaaac
    13141 ggtaatctct ccatttatgc ttcctccttc tgccgtgcca gcagaatctc tgcaatggcg
    13201 atgtcctccg gcgtggtgat cttgatgttg gtatagtccc cctcggtcat gtagacctta
    13261 cagttgatcg cctcgatcag ctggcagtca tcagtgaaat ccagtgcatg ctcctcggca
    13321 aacgtcactg cttcaaagta cagctgccgc ttgaacacct gcggtgtctg ggtgatgtac
    13381 aggtggggac ggtacggggt gtccacgatc agccggtcgt tgacaacttt aatggtgtct
    13441 ttcaccggca cgcccagtgt tgccccgccg aatacagacg cgtcctgaat cacctgtgcg
    13501 atctgctccg ttttgaccag cggtcttgca ccgtcgtgta cggcgatcat tgtcgtaccc
    13561 ttggacagtg cccgcagtcc gcacataacg ctttcctgtc gggtcgaacc gccggcaaca
    13621 atggcgtgaa acttggtgat gccgcactgc tctgcggttt cccgaatggc atcatggtcg
    13681 gcagggcgtg ccactacaat gatctctgcc actctggggc aggcttcgaa tgcacgcatg
    13741 ctcataccga tgacgttagt atctcccagc gggagaagaa tcttattttt tccctgcatt
    13801 ctggtggcgt ttccggcaca caccagaatg acagaggtgt cttgtgtctg tgacatagtc
    13861 gtctttcctt tccatctttc caaaaaaggg cacaggttct tattgaaacc ggtacccttt
    13921 tggcttatag attatgcagc ttataagaaa gcgtcatcag gctgtcgtcc agatgcatgg
    13981 tcacaacctg tgcctgctcc acggcaaggt cacagtgact gaaattccac agtccccgta
    14041 ctcccagcga aaccagccgt tccgccactg cctgtgccga ttctttcggc agacacagca
    14101 ccgcggctgc cggctgattg gcagcacaaa aatcatccaa ttcgtccatg gactggatcg
    14161 gcagtccggc aagctgtctg ccgatgagtt ccggcttgat gtcgaaaatc cccaccagcc
    14221 ggaagccacg ctgttcaaag cggacgtttt ccgaaaccac ttttcccaga ctgcctgcac
    14281 cgaccacgat cgccggaagc tgttccgtca gacccatgat cttctgtaaa ttttcccgca
    14341 gttccgccac ggaatagccg ctgccctgct gtccgaaaca gccgaaacag cagagatcct
    14401 gccgaatctg cgatgccgtc atacccagcc gcctgcccag ttcccgcgaa gaagtgcgaa
    14461 cctgtccgtt tcgttccagt tcgcccagaa gccgataata acggggcagc cgccgcacaa
    14521 cagacgacga tgcatcatat ttttgtgtca aacgaatcgc tctcctataa accgatttcc
    14581 attctgtaag ccggttctta cgccatcagc tttaccagac gctcgttgat tgcttccagg
    14641 aattccttgg agttgacctt cttcttgttc tccagcttgg agatcagata caggtcgcca
    14701 gtcatgacac cgtcttcgat cgtctgaatg gttgcctttt ccagcttgtc tgcaaaagcg
    14761 atcagttccg gcaggttatc catttcgccg cgtttccgca gtgcaccgct ccatgcaaag
    14821 atcgttgcca cagagttggt ggaagtttct tcgcccttga ggtacttgta atagtgccgc
    14881 tgcacagtgc cgtgtgcagc ctcatattca tagatgccgt ccggagaaac cagaacagaa
    14941 gtcatcattg ccagagaacc gtaagcggtg gaaaccatat cggacattac atcgccgtca
    15001 tagttcttgc aagcccagat gtagccgccg tgagaacgaa tcacacgagc cactgcatcg
    15061 tcgatcagag tgtagaaata ttcaatgcct gctgcttcga acttttcctt gtactcgttg
    15121 tcgtagatct cctggaaaat atccttgaag gtgtggtcgt acttcttgga aatggtatcc
    15181 ttggttgcaa accaaacgtc ctgatgcaga tccagaccat agttgaagca tgcccgtgca
    15241 aagccggcaa tcgagttgtt cttgttgtgc agtccctgaa tgatgccgtc ggtatcctca
    15301 aagttgaaga tcagttcacg gctctcgttg ccctctgcat cggtaacgac cagttctgcc
    15361 ttagagccct gctttacctt catttcggtg ttcttgtata catcaccgta agcgtgacgg
    15421 gcaatggtga tcggagcagt ccaagtcggg atatacggct ctacgccctt taccagaatc
    15481 ggggtacgga aaacggtgcc gtccagaatg gcacgaatgg tgccgttcgg gcttttccac
    15541 atctgggaca ggttgtattc cggcattctt gcagcgttcg gggtaatggt ggcacacttg
    15601 acagcaacac catacttctt ggtggcgttg gcactgtcta ccgttacctg atccttggtc
    15661 ttttcccgat attccagccc cagatcgtag tattcggtct tcaggtctac atacggtgtc
    15721 agcagaatgt ccttgatcca ctgccacaga acacgggtca tctcgtcgcc gtccatctcg
    15781 accagcggcg ttttcatctg aatttttgcc atttgatttt cctcctcaaa ttgtttctca
    15841 ttgcgtttag tttctgttca ggcattacaa tgtttccgcc ggagcaatgc ggtatccggc
    15901 ttgttctgcc aacgcagaaa ttttgtcata ttccatggtt gtcaccaaaa tcaactgtaa
    15961 gtcatcactg tcaatgtcca cgccgcagag cagcatgtcc tgttgccgga gatacgcatt
    16021 gcagacggca caccattgga acacgtcccc gtcctccgac aagtccagtc cggaaaggtc
    16081 cgacttttca ccgccgttca gccgggacac tgcccagagg aaatcctccg gttccgagct
    16141 gctgtccaca ccgatcaggt catgcacggc ttccagttcg tccaccatgg cgatccagca
    16201 cagcgtgtcc gcatcacagg ctgccgcatc tatgccgcgt tcctcgaaac gctccgcaaa
    16261 ccgtgccgca taggctgccg gttcgttcag acactgctcc agatcggcat gcactgccgg
    16321 attactgcgg ctgatgcagt cggctatcgc cagaacacac ggcatatcag atacctgctc
    16381 cggtgctttt ttctttttga agaatccgaa catgcttgtc cacacctttc ctacaggctc
    16441 ttattttttc gcatggtaca gtgcccatgc aaacaagccg ccgcccagaa gcgttgccac
    16501 gcccagaacc ggacgctgca agtagaactg caccacctcc ggcatttgcc ggaacagcaa
    16561 aaactgacgg tacagatacc acagtaaaac gggaagtccc actgcgattg ctgaaaaagc
    16621 aattgcgtat tccagaactg ttttccgttt cattttccgc cgatgcttct gcacggattt
    16681 tcgcatggtg ttgtcctgca tggcatcttc catggaaaat atccgcttac ttcgggaaat
    16741 atcttccgta aactgctttg ccagatcttc gaaatgcagt tcctgctgcc tgtcctgctt
    16801 catttacgca aacaccaccc aacgaagaat ccccagcacc agcaggtgca gcggatagaa
    16861 cacatagaaa aaccacttgt gaaacggctt tttgctgccg ctttcgccgt tgtagcaata
    16921 ttgtattaac ggtatcacca gaaagatgcc cagctggaac agtccgctcc atatgggttc
    16981 atttcccagc atgctgacac agcacaccag tgtaatgccg ctgaacgtca gccatttttc
    17041 cctgggattc tcccgatacc ggaaaaaggt cagtgcatac agcacatcga acatcgccca
    17101 gtcaccaaac acagacagga tacagagcac cagcactgcc agccatttta cagaaacaga
    17161 gcatttcgcc ttgtcccaga accagatcgc cagcaggctg aggaacagcg tatagatcac
    17221 gccgaacgcc ggacgaatgc cgccgctttc gaaatagctg aacggcagcc acgagatcac
    17281 ggcaaaaata cccagccgca tggcgtattt ctttacgctg cgggtatagt gatacccctc
    17341 cgccagcata tacgccatgg tcggaccggt cagccgcccg atgatgtgca tcacctgccc
    17401 cagcagcgac gctgtaggaa caaatgccca cgcaatgtgg tcaatgagca tggctgcgat
    17461 caccagatat ttcagctggt tgcggttgag aaatcctttt gtgtttgtca ttcccgtttc
    17521 tcccgatcct gccgcagttc ccgcagcagc tttctcagca catacagacc ggcggcaacc
    17581 agcagcaaca gtactgccag cccgattccg gaaaccggac gttcccgcag cagatgcaaa
    17641 tagtccatga tgtcatgatc ggtaagcatt gcatagacaa agacgatcat caacacggca
    17701 aacaaatgat tcccgtaatc tttccggaat tgccggtatc tttcgatttt ctttttcgtt
    17761 tccggatcag ccacttactt gctcagcttg tcgcgggtgt aatccagcag accgcctgcc
    17821 agaatgatat ccttggtgcg tccggtcagt tcgcacagta ccggaatctc tgcaccggta
    17881 gtcttgttga ccagtgtcag tgtggtttcg ccggcttcga catgcttgcg aatatctgcc
    17941 agttccagta cgtcgccctg cgagatcttc tcatagtctg cttcgttgac gaaagtcagc
    18001 ggcagaatgc cggcgttgat gaggtttgca cggtggatac gggcaaagct cttgaccaga
    18061 actgccttga cacccagata cagcggagcc agtgcggcat gctcacggga agaaccctgt
    18121 ccatagttgg agccgcctac gataatgctc tgtcccattt ccttggcacg cttcgggaat
    18181 gcttcatcgc aaactgtgaa gcagtgctgc gaaattgccg gaatattgga acgcagcggc
    18241 agaatctttg cacctgccgg catgatgtgg tcggtggtga tgttgtcgcc gaccttcagt
    18301 gagcagccag cttcgatcgt tgcttccagc ggagccgttt ccggaaacgg cttgatgttc
    18361 ggtccgcgga ggatctccac cttgtccatt tcttcttccg gtgccggcag aacgatcatg
    18421 ttgtcgttga cggtgaattt ttccggcagc acgaagtccg gcatttcgcc cagttctctc
    18481 gggtcgctca gataaccggt cagtgcagaa tatgcagcca gttccggaga aaccagatag
    18541 atctgaccgt ccttggttcc ggagcgtccg aggaagttcc ggttaaaggt acgcagggag
    18601 ataccgccgg agttcgggga ctgtcccata ccgatgcacg gaccgcaggc actttccaga
    18661 attcttgcac cggctgcgat cagggtgccc agaatgccca tatctgccat catgttcagc
    18721 acctgcttgg agccaggagc gattgccaga gaaacgtccg gattgacagt cttgcccttg
    18781 aggatatgag ccactttcat catatccagc agggaggagt tggtgcagga accgatgcag
    18841 acctgatcga tcttcagctt gccgatctct gccacggtct tgacattgtc cggagaatgc
    18901 ggacatgcag ccaagggcac cagttcgctc agatcaatgt gcagttcttc atcataaact
    18961 gcatcggcat ctgccttctg ttcgctccag acttcgcctc tgccctgtgc ttcgaggaac
    19021 tgcttggtca cctcgtccga cgggaaaata gaagtagtcg cacccagttc tgcacccatg
    19081 ttggtaatgg ttgcacgttc cggaacagac agggtcttga caccctcgcc gcagtattca
    19141 atgaccttgc cgacaccgcc ctttacggac atgcgacgca gtacttccag aatgacatct
    19201 tttgccgcta cccatgggga cagcttgccg gtcagttcta cctttaccac cttcgggcag
    19261 gtaatgtagt aagtaccgcc gcccattgcc actgcaacgt ccagaccgcc ggcacccatc
    19321 gcgatcatac cgataccgcc gccagtcggg gtatggctgt cagagccgat cagggtcttt
    19381 cccggaacgc cgaaacgctc cagatgtacc tgatggcaga taccggtgcc gggacgggaa
    19441 aaataaatgc cgtgcttctt tgcaacgctg ccgataaagc ggtggtcatc tgcattttca
    19501 aagccgttct ggagggtgtt gtggtcgatg tatgccacgc tgcgttctgt acgtacacgc
    19561 ggaacgccca ttgcctcaaa ttccagatat gccattgtac cggttgcgtc ctgtgtcagg
    19621 gtctggtcga tccggattcc gatttcggaa ccctttacca tttcaccttc caccagatgt
    19681 gctttcagga tcttttccgt cagggtcagt cccattcaat atcattcctt tcgtagttgg
    19741 cactgcaaat acacggcaag ccatacattc acaagtacga ttccttaggg aaatgccgca
    19801 taaagctgcc tgtcggtgct gcccttatcc gacatttcca ttatactgta ctattatagc
    19861 aaaaaacagg cgtagttgtc aagtctgcgg cagcggcttt gaaaagatgc aaaggaaagg
    19921 ataaaatttt aagaaaaaac agaagtcgtc cggcatctat aacgcaaaac aacccgcagg
    19981 cacacacctg cgggctgtca catattcgtc aatattttct gttatgctcc ggctttttcc
    20041 agactgtaca catggtcgcc gcttagccgc ttttcgaaat cgtcgtgagg cagcggacgg
    20101 tcgaacagga agccctgtgc catacagcag tgcatgcccc gcaggaattc cgcctgcttg
    20161 acagtttcca cgccctctgc aatgacttcc atgtccagtt cattcaccat tttgatgatg
    20221 ttcttgatga cgatcacatc atttttccgg tgattttcca agctgctgag gaacgattta
    20281 tccagcttga taatatccac gttcaggtct ttcagcagat tcaacgagga ataacctgta
    20341 ccgaagtcgt caatagaggt gctcacgccg ttggcacgca ttgccctgac gaaattgagc
    20401 agggtttcat agttctcata gccggacatc tcggtcagtt ctacttcgat atacttggga
    20461 ggaatttcgt atttctgaag caccgccaga atttcttctg ccagatgtgg attttgcaga
    20521 tgcaccttgg aaaagttgac agagatccgc accggttcga tgcctttcgc cttccagtcc
    20581 cgcacatctc ggcaaacctg ttccagcaca taaaagtcca gcttgcagac gctaccttcc
    20641 cgttccagca ccggaatgaa gtccatcggc ggcaccatag caccgtcacg gaaccagcgt
    20701 actaacgctt cacagccgca gagctggttg tccaccaagg tgactttcgg ctgatagtat
    20761 accacaaact cccgatctgc caatgcctta ggaaacatct gggaaacttc tttctgccgc
    20821 agggcattct ctaccatctg gtcatcgaag cagaaacgat ctgcttttcc ggacatcttt
    20881 gccgcatgca ttgccgtaga agctgcattg aggccttctc ccatagactg tcccgcccgc
    20941 atatcataaa cgccgatacg ggaacgcagg tcaaacgttt tggtgccatt ttcgtaaggg
    21001 accgtcacct gcatacaatc aatttgttcc agcagtattc ccatgcgttc tttttttgcc
    21061 agcagcacaa agttgtcgcc gcccatgcgt gcgaaatatt cgtctggttt cagcttctgc
    21121 cgtgccagcc gtacaaattc ccgcagcacc atatcaccct gccgtgctcc tacggcacgg
    21181 ttgatgtaat tgaaattctt aatgttcaga aatgcacagg catactcctg caatttcccc
    21241 tgtgccagca gttttccgcc ggtttcgttg atgccgcggg tgttgagagc accggtcagc
    21301 agatccgtca ctgcaatgca ttccagaagc tgcaccagct ttacccgttc cagcagcagg
    21361 aacaactgac gggacagaaa caacagcatt tgctcctgta tcggactcca tgcagtgcct
    21421 tttacgggat aatattcaaa aacggcacta caggtttcgt ccatctgcac atgttcccga
    21481 cagcaaggct catcaaaacc ttcttcattc tcatagagca agaaccgttc gtcctgtccc
    21541 tcaggatcca gtatagacgg agccacgatc aaacggattt caaactttcc cagcggaaaa
    21601 agacgcacta tttcctgcat ctgctctttc agcagcgaaa acagttcttc tactgttttc
    21661 acgttcttct gcagtttgtc gaacaactga cagtaactct tcacatgaaa gtccatatcc
    21721 atacaatcac ctcgttatgc attttcaacc ctttctcgaa atcaacctct atatattttt
    21781 actataacac attttttgca ccttgtcaat caatgacaga aaacaaattg acttttacag
    21841 aatgtttgtc cattatcatg aaacatggaa gtgttttttg gcgtttccta caaatttgac
    21901 aaatcgcccg aaatgcgtta cactagggag tgttgatact ataatgatat gcgaattttg
    21961 tgaggtgatt tttctccgtt ctaggcaaaa attcgcaggc gtgctttcgc acggcaagga
    22021 tttttaacga cgaacgggga aaaatcaccc caaaaggcac gtgtcaggct tagtgtcaac
    22081 accccctaga gtatgttccc ctaggggaac cgctgaatat ttcatagaac ggacgtgaaa
    22141 cctgtgcaaa ctaccctgca aaatctttca ttcagtctga atgccaccat tccgatcttt
    22201 ctcatgatgg tattcggcgg tctgatgaaa aaagtcggac tgctggacga ccacaccacc
    22261 gccaagctaa accagtttgt ctttaaggca ctgctgccgg tactgctgtt catggacttg
    22321 tccaaggcag attttcagac agtgtgggac ggcaaattcg tgctgttctg cttttttgcc
    22381 acactgctta gtatcggcat tgcctttctc ctgtccctgc cgcgaaaaga ctgggcagaa
    22441 cggggcgaga tcattcaggc atcttaccgc agcagtgccg ccattctggg gattgccttt
    22501 gtcaacaaca tctacggaca cgccaccatg gctgccctga tgatcgtggg gacggtaccc
    22561 ctgtacaaca tcgccgccgt catcattctc tccctgacgg cacctcacga cgatgactct
    22621 cccaacgcca agaagctgct gctgcgaact ctgaaagacg tggtgaccaa tcccattatt
    22681 ctgggaatcg cagtgggcat gctctggtct gtcctgcaca ttccgcagcc gacgattctg
    22741 caaaagtcgg tttcctatct gggaaatatg gcgacaccgc tggcactgat cgcactggga
    22801 gcatcattcc ggctggagaa cgccaagggc aagctggggc tgacgctggg catttctgcc
    22861 gcaaagctgt tcctgttctg tgccctgttc ctgccggcag cgatctggct gggattccgc
    22921 acggaaaagc tgatcgccat tctggtgatg ctgggaagtg ccaccacgtg cagctgtttt
    22981 gtcatggcga aaaatatgaa gcatgacggc accattaccg cctgtgccgt gatgatgacc
    23041 accctgctca gtgcgttttc gctgacgatg tggctgtttg tgatgaaaag tctggggtat
    23101 atttgacagt cgttcggaat tggaagaaaa attctgaaat tcccgttcca acactgtagg
    23161 catttttcag tatgttctaa acgcttttat tgctttgtca aatcccaatt aggaagaaga
    23221 tagtagattt tatctgacat tgtttcatag aaattctgtt cataaaagtc tgtgatttgc
    23281 cattttccat tctgtttttc tattttaaat tttgttccca tcttaggacc gccttgattg
    23341 tctgtcatta tattgccgtc cttgtcaaca acatcgaagt gaatcacaac ccaaatgtat
    23401 ccggaatgaa aattatgcag cgtgtatgtg cgatgaacat ctatgttttg tattttatag
    23461 ccctctaaat attcttcgat tgacatttta cgtgtagggg aacaatacag ccgataatca
    23521 tcaaaaattt gattccagtg atattcttca tatttcactt gattttcgtc cagcaataga
    23581 cgttcttcca cggcaagttc atgttctgac atcacataat atgtatcatt ctgtacagca
    23641 ttttctattt ccaaaacaac ggcatttgta ctttgctgca tcattatcca tatcgttgtg
    23701 attccaagcg tcaaaagcac gataataaaa attatcactt tacttttttt gcaacatctt
    23761 cgcatttcct caccattatc cttaccacat caaaatccaa accgccggca cgcagatttt
    23821 tctgtacgcc ggcggtttgc tcatttatcc gattttctcc ggctcacact tcattttcct
    23881 gcaattcctg aaagaatgtg aaataatcct gccgcttttc attggtgaat gcgatcgccg
    23941 tccgcggatg ctggttcagg taaccggtca gcaggtactg aatgtcatag ccccagacaa
    24001 cgccgtcatt tttcagcttc tgcatttcgt cctgcaaaaa ccgcagcacc ggcttgatgt
    24061 catacttcgg atttttcagg aatcccatca gcagttccat tgcacagttt ccggcaccgc
    24121 gtcccatggc agccatggtt gcgtccagat agctgacacc catcatagca cactcggtag
    24181 tgttggcaaa tgccagctgc tggttgttgt gggcgtggat accgatcttc ttgccgtatt
    24241 tttcgccggc ttccagatac cgctgtgcca gctgacgcat ttgcagcgga tacagcgaac
    24301 cgtaactgtc taccagatag atgacttcca cgctgctctt gcccagcatt tccagtgctt
    24361 cttccagttc cgattcctgt gccttggaaa ctgccatgat gttcactgtg gtgtgatagc
    24421 cctttttcgt acaggtttcg atcatgtcaa tggctgccgg aatggtgttg atataggtgg
    24481 cgatccgcac catatccaga atactgtcct ttttgtccgg cagatcccgt gccacgttgg
    24541 cacgtcctac gtctgccata actgccagct gcatcttcgg gtcgagaatg tcgatcagca
    24601 ccttctgaat ggcatcttca tggcagaact tccacttgcc gaacttggat tcctcgaaca
    24661 gctccggatc tgccagatag ccgatttcca tcacatcgac accggcacgt acattggtca
    24721 gatacagctt ccggacaaag tcgtctgtaa actcaaagtt gtttaccagt ccgccgtccc
    24781 gaatggtagc gtccagcact ttgatgtccg gacgatagga gatcatggta ccctttatca
    24841 tatcgctcat tggagattcc tcatttctgt gtcattttcc acagccgtgg tgactgcttt
    24901 tctttaagca ataccgcaca aagattgtgc gacagatgcg tcgtcaaatt tttcgtcaga
    24961 t
//
LOCUS       DAWBGR010000010        24729 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_755_length_24729_cov_4.777620, whole genome shotgun sequence.
ACCESSION   DAWBGR010000010 DAWBGR010000000
VERSION     DAWBGR010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 24729)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24729)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24729
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_755_length_24729_cov_4.777620"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5600 a         6694 c         6041 g         6394 t
ORIGIN      
        1 gtacggcaca cagctgctct accgaagcca cgcggaaagc cacttcacct gttccggttt
       61 cgtcatgaat ccggcaatgg atcaggtgct gatggtctac caccgcatct acgattcctt
      121 tgcatggaca ggcggacacg ctgacggttc cagtgatttt ctctggacag ccgtgcggga
      181 ggcaaaagag gaaaccggta tccagaagcc ctatcccctt accggagcca ttctctcgct
      241 ggacattctc cctgtcaaag cacatcagaa aaaaggcgtt cccgttccgg cacatcagca
      301 ctataacgtc acctacggca tcattgccga ccagaaagaa acgctgcgga tccagccgga
      361 cgaaaacact gcggtgcagt ggatccctgt ggaacaactg ccggaaatct gcaaagagcc
      421 ccacatgctg ccggtctatg aaaaagtgat ccaccgcatg cgttgctgga aagccatgca
      481 ggaacaggca cttctgcaaa cagcacagcc gctgttggcg tggtatccgc tgcatgcacg
      541 ggatctcccc tggcggcaca cgcaggagcc ttaccgcatc tggctgtcag agatcatgct
      601 ccagcagacg cgggtggaag ccgtcaagaa ctattacgca cggtttctgg aaacctttcc
      661 ggacattgcc gcacttgccg cagcggaaca ggatcaggtc aacaagtgct gggaggggct
      721 gggctattac agccgtgcag cgaatctgcg aaaggcagca cagacgattc aggagcagta
      781 cggcggcgtg tttccccgca catgggagga agtccgcgga ctggctggcg taggcgatta
      841 caccgccggt gcagtctgtt ccatttgcta cggtttgcct acgccagcgg tagacggcaa
      901 tgtgctgcgg gtggctgcca gagtgatgaa ccacttctgt gagatcgacc ggccggaaca
      961 aaaggcagct gtcactgccc tgctgcggca ggtctatctg gacaatccgg agcactgcgg
     1021 cacgctgaca caggcgttta tggaaatcgg agccacggtc tgcgtgccca acggacagcc
     1081 gcattgtgaa gcctgtcccc tgtcggaact gtgtctgggc agacagtact gcgatgctct
     1141 gcggctgccc cagcggactg ccaaaaaagc cagacgacag gaacaataca ccgtttttgt
     1201 gctgcaatgc gacgggaaat tcgccctccg gaaacgcact gccaaggggc tgctccacgg
     1261 gctttgggaa tatcccaatg ttgccggcat ctgcactgcc gaagaagcca ttgcccaggc
     1321 cagtgcatgg cactgcaagc ccacggatct gacgcagacg gtagaacgga aacacatctt
     1381 cacccatgtg gaatgggaac tctacggcgt atacctcacc tgtgccagac aggacgaaca
     1441 attcgtctgg aaaactgcgg cggaaattac caaggagatt tccctgccca cggctttccg
     1501 gcagtttttc taaaggcaat accgcacaat ctttgtgcgg tattgcccta aaatcaaaat
     1561 atcacaaaaa ccccctgccc ttgcagccat tgccgcaagg acagggggtt gattttcttt
     1621 cagaacaagt ggatttttat cagtaacacc gcaaaaagcc cacccgacag catttgcacg
     1681 caatctgcct gcaaatacca tcgtgaccgt caccggtaat gtgattttca ggcttcgtag
     1741 aaagcagtct ttctttccag cactttctta ccgatgggcg aactgatcgg agccactacg
     1801 caggaaccat acttgttgat tgcctttgcc acgtggtcag cgatctcacg gacattgttt
     1861 tcgttttcca attccaaaac taccacgaaa ccgtctgtgt tgttcgcctt gacaccgtcg
     1921 atgtatgctt tcttgacatc tgcattttct gcaaagtatg ctttcaggtc gtcttccatg
     1981 ccctccggag tggagtcaat atcccgcatt gccagacgcg gctgaatgtt cattgcctgc
     2041 tccttgctga atgccagccc catggtgaac ggatcgagca cgatgccggc agctgtgctc
     2101 ttgggatcag acagcattgc cacatagttt gccatgctca tgggaactgc cttgcagtcc
     2161 ggtgccccgt tccacttctg tagttctgac aggctggtga acagcggcat gtactttccg
     2221 ccctcgtggt tcagcatgat gaactctgcc tgtgcattga ccttttcgcc agccttgact
     2281 tccttcggca cttccatcag caccggagcc acaaagcgtg ctttccgcat tgcctcaaac
     2341 atattgttgc gggcaggttc gttgttttcc cgacgcagac ggtctgcggc aacgatcact
     2401 tccggattgg tgaccttttc gcagccaaac agcgggagaa tgttgttctt cttcggagcc
     2461 tcctgcggct gttctgcctc cagagaaacg cccttcatct ctgccatttt ttccttgcgg
     2521 atcacgatgt tggtttccgc cggattgatg acgataccgc tgacatcgac accctgcttc
     2581 agcaggatcc cgcagtactg gtcaaatgta attaccattg tgtccgcgtc cggatcgttc
     2641 ttccacttca ggaattccag ccagtcggtg aaagccggaa agaactttac accgtccttg
     2701 ttggaaacag ccatgagaga agcctccatg tgacgctgac cattctcgtc cggttctacc
     2761 tcgttcagct tgttctgcaa cgtgatcgga gcaataaacg atgctgctgc aattgcgtcc
     2821 agcatttctt tttcggtctg tacgctctgc tgttttttga attccgccat tgcggcaacc
     2881 agatcagggt ttttgattgt gggttttttc agttgtgaca atttgattca cctcatctaa
     2941 aatttatcgc ccgaaaacgc cgccgtgccc gcaggcagcg gaagcgacag ggatagaaat
     3001 gaaaacactt ttccatgtca gacttccaaa tccggcatgg gtgctgtcta tagtataaca
     3061 tatcccgtaa aaaaaagcaa gtcaaattgt acaaaaaaac cgaaaaaagc ggaattgctt
     3121 ttcacgcaaa aatggcagcc gaaaacatcg gctgccattt gcagaataaa gatctatttc
     3181 ttttccggag aatcggttat tccgttaccg gcagggaggt aactatgcgt accaggaatt
     3241 gcagcagaac tgtcgaatcg ttggtagaga gttctccgtc tgccatgcag tcggcattct
     3301 tctgctggct ggcgttcagc gttaccacac cggcaaccgc cttgttcagc agaactgcat
     3361 cggtaatatc caccttgccg tccaagttca catcgccgta cagggactga gaggtcactt
     3421 ccgaagcggt ggtctgcgtg gtttctgtgt tgctgtcgcc gctgctgact ggtgtagtgg
     3481 cagtcgactg ttccgtggtg tccgccgtat ccgaagttgc cggagcagct gtggtttcag
     3541 tgggctgtgc ggaagtttca ctggtcttta cagcagtttc tgtcggctgt gcagaagttt
     3601 cgctggtctt tgcagaagtt tccgaaccgg tagtggtttc agtgggctgc actgctgtgg
     3661 tagtagtagt ggtggtttgc tgttcagaag aagtgcttgt ggttgttgtc ggcggaacga
     3721 caatgctgcc gttatacata tggtaaccca ttgtcttata gctgctggaa tccgcagttt
     3781 catacaaacc gcccggattc agcgaaccgt ctgcatttct ccatacagca tcaaaatcgg
     3841 taccctgttc cggagccttt gccattgtga taaagtcaga agctgccgga gccgcagaaa
     3901 gctttgtgcc gatcttcgca ccgtttgtga tcgcagtctt tgcatctacc tgatagtagc
     3961 cgctgttgta gtacacgcca ttttcataag taccagcata cttgtcgtta gaagcaccgc
     4021 ctttcagctt tgcgtcagac ataaatacag aatcatcata ataggacatc atattctcaa
     4081 agattgcacc cggatctgtg cagcgatagc aggagaagtt cggctttccc ttgccttcgc
     4141 cgttgttgtt gactgcggta cagttggtca gactgcccag cttcgggtta ttgttatcgg
     4201 tgaagccgca gtgaaggttc tcgaatgcca gacagttctt caagatatgt gcagaaccga
     4261 taccagagcc gcccagcttg aagccgttgc cgtcgcaatt gccgtagccc tcgccgaatt
     4321 ccgtaaagcc gttgcggaat gcaatacaat tctgaatggt gacaacaccg ataggaccgg
     4381 tagcactttt tgcaaacaga tcccagccgt cgtcactgtt gttgtatgcc atacagccgt
     4441 caaagacgtt gccctcgccg caggtcagct ttgctgcaaa gccgtcagca ttttccatgc
     4501 tggcattgtc gcagttgttc ttggaagtgc agttcaggat caggttattg ctgggccagt
     4561 cggcaatggt tgctgcactg gtattataac gggacagctg caaaccagtg tcctgattgt
     4621 cgttgaatac catgcgttcg atcttattgt tgttgccgga gaggagcatg ccgttatcgg
     4681 ctgccttagt gatctcaaaa tcatagaagt gccagtagtc accgtccagc acaatgccac
     4741 gcttgctgcc gtctgcatcg ccctgtccgg agaagtcgaa caccacatct gcaccgttat
     4801 aaggcttgat cgtcttcatt gcattagcag taccgctgtt ctgagcgtca atgaggatca
     4861 tttccgagaa cttgtatgta ccgcccagca gataaatggt gtggcctgcg gacagcttgg
     4921 agatcgcatc ggtaacagaa gccggatcct tttcggaaga accgctgccg gtaccggtag
     4981 gcgaacagaa gatgtcccct gccaccggca cgtccggtgt ggtaggcgtg gtgttcttgg
     5041 tggtagcagt ggtcagagcc gttgcggtcg tatccttgga cacgctgacg gtggtggtca
     5101 ctgtagtagt cttggtttcc tcagtggtga caaccggatc tgtggtgctg tcggtaaagc
     5161 cgctgccgat cgccacgata gaggacttgt aattgaccag agcgtctttc aatgcctgat
     5221 tgaccgcata gctggtatcg tccacggagt tgtcaaactt ccactggaga tcaccgccct
     5281 gtacgcgtcc ggccttggca gtaacgatag ccggcacatc agccgctgca tctgccttgt
     5341 agctgtacat gatgctggaa tcggtatcga agttgttgta gcctgtgccg ccggaaaccg
     5401 tcttgacgct gtccggtact ttttcagacg ggctggaaac ttcataggca tcgaacgagg
     5461 tgctgttctg cgaatacggg atatagctgg aagcaccctc gatatagttg ccgcaagcct
     5521 tgatgatacc gccggcttcg ccggagaatg taccttcacc cagtgcatca gtaccctgtc
     5581 cggaactcat catcgggttc ttacagttgc ggaagtagtt gttttccgcg aaagcagaag
     5641 atcccatggt gacgccgatg ccgtacttgg cattgccgtc gaagtagttg ttatagctgt
     5701 gtacagagca ggtgcggatt ctggggtgac gggaatcaga gtgatcgtac cagttgtggt
     5761 gataggtcac atagttggtt tcctcttccg atttcatgcc ctgcaggttg cactttccgt
     5821 tgtcccagaa gtggttgtag gaatgtgtta cataggtgga agtcttggtg tccagtgcac
     5881 cgtcgccctt gacctgatca gcatctgaac cggcatcacc atagaagaaa tcacagttat
     5941 gcacccagat gtggtcgttg ccctgctgca agccgcagtc atcgccctcg ctgctgttgc
     6001 agttcatgaa gccgatattg cggacttcta cgttagagca gttcttcatg accagaccaa
     6061 aaccgttgaa tacggtatcg gtaccaatac cctccacggt gatgccagcc tttgcggtgt
     6121 cgatcatcag atcgcccttg ggcatttctg ccggatcggt gatgttgccg ataaaccgca
     6181 ggcacagcgg acgcttttct ttatttttct tataagccgt aatgatgttc tgcacaccag
     6241 tcacatcggt cgcacccttg ccggtggagt caatgcttgc cgtcacggtg tccttgttgg
     6301 catcggtgac atataccaca atggcatctg ctttcagcgt gccgtcctcg ttgtatgcac
     6361 cggaagaagt gccctttgca aagccaaaac cgcttctgtc gtgagcctct acctgaagtc
     6421 ccgcagtttc tgctgcctta gaagcatctt ccttgccgtt aatcaccggc acgatcttca
     6481 tggtgtagct gccggctttc agacctactg catcagcacg gaagcgatcc ggatactggc
     6541 gtaccagcat ggtatccagc tgcgtgtagc tgccgcctgc cggcttgata tacacgttgt
     6601 agccggtcgc attggataca gcaccccatt ccgcataggc agcttccagg tcgccgctgg
     6661 catcgatcac agacaccgaa gcactggctg ccacaatcgc cggaacgttg tcagttacag
     6721 ttcccagatc tgctgccaga accggctgtg caccggctgc ggcagtggcc gccgccagca
     6781 gaaatgccag aatactggtt ccgatttttt tgttcttcat cattcattcc tcctgttcgt
     6841 ccgatgtgtg catacaccgg acatcagctg aaaaatttcc ggaagcctca ttgcccggaa
     6901 tatttctcat atttctcctt atagctcaaa ttgtggtgga tagctcacat tgtggtgaca
     6961 catttttcca cccaatttcc acctccattt tacgcctttt tccctgtaat gtcaatctgc
     7021 tttttcaaca aaaatgcaat gttttcagtg tgcacattac acaacatatt tgcacttttt
     7081 gttagttttc atcgcataaa gtaacagaat atatgactga ttttccgcat acattacaac
     7141 agcccgccga taaaatttca atgtgcattt ggaaacaaat cggtaaagga tgtttacaat
     7201 ttatatttca tgtcgaaaag tgttgacaaa cagaaatcca gtgggtataa taataaagaa
     7261 tggctctggc gaatatccac gctgcagagc ccgcttctct caatgtaata tttttttaat
     7321 ttttccagaa acagaggttc tactgtcttc ggactgaccg cagctgtcat cagtttgtca
     7381 acatactcgg aacgccgaca cgcggtgaag ttcaatgatg ctgtattccg cacagccctc
     7441 gtaccatgga agaaaggaaa gatgacagtg aacaataaag accaatcccc tgccggaaat
     7501 gcttccaagc agcatctgac caaagacgaa atgcgggaaa aaatcaaagc ttctctcgct
     7561 gcaaaagagg caacagaatt tcagtcatca tcgccgaagc cgtcttctga acgcttttcc
     7621 ggacagaatc gtccggcttc ccgcagccct gaaaacagct ccgcttcgga acgcacccgt
     7681 tcggacaagg cacggcagat ccatgatgcc gtttcttccg gctatgccta tgaccagccg
     7741 cggcagcagc gtgcaccaca gccgcagcac cagcagcgtt ccggaggcaa cgtggcaaca
     7801 gcacagcgtc cgccccgccc tacaccgccc agacattacg aaccagtgcg ggaggagtcc
     7861 aacgccggaa agatctgcat catcattggt attgccgtgc tggctgtact cctgctgagc
     7921 tacatcgccg gactggctgt ctatcacagc aagtttctcc ctaaaactta cgtcaacggc
     7981 gtggacatcg gcggcatgac cgcggaggaa gccagcgacg cagttctgaa taccgcacag
     8041 gatatggggc tgacattcat tcccaaaagc ggcgacccga tcaccttcaa gggctcctca
     8101 ttcggctgca ccgttaccct gccggacaac gctctgacag agccggcaga cgaaagtcac
     8161 gccctctggt tcaggaagct gttctccaaa accgagtata cagtcaagat gcaggattcc
     8221 tactcggaag atgcactggt cagcctgatc gccgcctatg actggggcaa tgtaccgccc
     8281 accgatgcca aggttgtcca gaacgaggac ggttccttta ccattcagcc ggaagataac
     8341 ggcaacatgg tagacaccca gaagctgtcc gactacacgg tcgcacagat gcgggagggc
     8401 aataacacca ttcagatggc agacagtgac tgctacaaaa aggcagctgt cactgcggaa
     8461 agtctggagc ctacactggc actgtacaac aagatcggtg ctgtagagat cacctatgac
     8521 atgactgacc gcgaggaaat tttcgatccg gttggcacgg aaaagctgga tcacgctact
     8581 atcatggact ggatcaccac ggacggcgat gacatcactg tagacaccga caaagcttcc
     8641 gcatgggtgc aggaacatat cgctgacaag tacgacacac tggtgaccgg ctacaacttc
     8701 aagttcaaag ccaccgaaga cggagagatt accctgccaa tcggttctga cggcatctac
     8761 ggctggaaaa ccaatgtttc tgccactgtg gaaaagctgg tagattacat caaagccggc
     8821 gaggcagtaa ccattgaacc ggtatataag gtagagggat tccgcatgaa ctccaacgcc
     8881 ggcgttacct acaccggaaa cacctacatt gaagtggaca tctgccacca gcacttgtgg
     8941 tactatgtca atggcgaact gttcctggag tccgatgtgg ttaccggtaa ggagtccgat
     9001 ccgacacgtg ccacaccgcc gggagcatat aaggtatggt ccagagaatc tccgcgaaag
     9061 ctggggactt acgcagtgca gggctatgaa acatgggtaa attactggat gcccgtcacc
     9121 tataccggca tcggtctgca cgacctgaac cgcagtgcct acggcggtga catctacatc
     9181 aacaacggtt cccacggctg tatcaacctg ccgctggacg ttgccaaaaa gatctatgac
     9241 aaggtcacta tcaacacacc ggttatgatc gttccgtaaa ccgcagccac tataacaacg
     9301 aaaaaagcgt accgttttcc tgtgaaacgg tacgcttttt gttatggcat cacggcacaa
     9361 aactgtgcgg tatcgcctta tgtttgcttt tgtattatcc gcggcgtact gccggcatca
     9421 tgctggtgaa tgctgcttcg tcttcggtaa cttctccctc cggatactgg aaccggatca
     9481 gacgatcccc ctgaaaagtc agctgtccct gctgatggaa cggggtttcg atatacgctt
     9541 tcttgctggt aacaatatgc agcacatcac ccttgcgggt cttgaaaatc aactcatagt
     9601 attcgttgta cagtttcaaa gtgccgctgg gatccatggt tctgcggcgt tcgtctacct
     9661 tttgtaccag aacagcgatc acggtacggg gtggattttt tccgcggttt ggtctacgca
     9721 ttgctctttc tcctgtgata taatattccc gattaaactc cagcggctgc cgtttgtcgg
     9781 cagtgtttgg attctgtacc agaagccggc tcagcttctg ccggaacggg ctttgccgag
     9841 aacccagcat caccaccagc agcagcacca cggcaacagc aaccatgata atcaggaaat
     9901 tatcgatcgc atttccgaat ctgcctgtca tatgtattcc tttctgtggg aacgcatttt
     9961 cgtaaacgga ttacgaaagc agcttctcac attccattgg gattctgctt tgtgaaacgc
    10021 cagctgtcgg cacacatctg ctgcaaatcc gcagttgctt cccagccaag cagttccttt
    10081 gctttcgacg gatctgcata cgaggtggca atatctccgg cacggcgttc cgtgatctct
    10141 accggaatct tcttgccgca tgccttttca tacgcatgca ccacgtccag cacgctgctg
    10201 ccgtgaccgg tacccagatt gatcggttca aagccacggt gctccagtcc gtatgccacg
    10261 gctgccacgt gaccggttgc cagatccata acatggatat agtcccgcac accagtgccg
    10321 tccggcgtgt cgtagtcatc accgaatacc cgcacatgat ccagcttgcc cagtgccacc
    10381 tgtgccacat agggcaccag attgttgggg attccgttgg gattctcgcc cagcagaccg
    10441 ctgatgtgag caccgatcgg gttgaaatac cgcagagcca cagccgtgaa ctccggattg
    10501 gcagtgcaaa cgtcccgcag aatctgttcg atcatgacct tggtgtagcc atagggattc
    10561 gttgcagagg tcggcatttc ctccacgtag ggagcctcat tgttgcagcc gtaaacggtc
    10621 gcactggagg agaaaattat cacattgcag tgatactccc gcatgacacg cagcagggag
    10681 agtgtaccgc tgatgttgtt ctcgtagtat tccagcggct tttccaccga ttcgccaact
    10741 gccttcagac cggcgaagtg aattacggca tcgatcttgt tgtcctcaaa aatctgccgc
    10801 atgtcctcct cgtcacgcat atctgtacga tagaaccgca catgctttcc ggtgatcatc
    10861 tcgatacggg caattgcctc cacctttgaa ttgcagagat tgtccataat cacgacctga
    10921 tagccggcat tgatcaatgc cacacaagta tgactgccga taaagccggt accgccagtg
    10981 accagaattt ttcccatgtt actttacgct tcctttcaat ttctgtcaaa tggagaggga
    11041 attttccctc ctatcagttt gccctgaaag gcaaactttc agcaacactg cacaaaagca
    11101 caatgctgcc ttatatcaat acagtcatgc cgccaacgga acgaattcgc agcacgtggc
    11161 agcagccctt tcccagaacg gattccatac cctcctgaaa ctgggacagc agcacagccg
    11221 gcacaaatgc ctgtactgtt ccgccgaaac cgccgccgtg tacacggatc gctcccctgc
    11281 cgcccagcac tgccgagccg actgcaaggg caatggagat gggctggtct tccggtgcgg
    11341 aggacgggta gacgttttgc agcaggcaat aggaagactg tccggagttc tgtaccaggt
    11401 gcaggaacgc ctcgaaatcg ccccgatcca gtgcatcaga ttcgccctgt acccgtccgt
    11461 cttcccgata aaagtgcatc gcacggagca ctgcacggtc gccgcaggct ttccgcagtt
    11521 ccggaatgga acgcagcacc tgtgccgacg gaacttccgc cagaacgtcc ttgccgaaat
    11581 actttgccac ttcctgcatt tctgccggaa tcgcagcgta ttccgctgtc aggtctgcgt
    11641 ggtttccgcc ggtatccaca atgcaaagta cataaccggt tctggaaaag tcaaatgcaa
    11701 ctgggtgcac tgccggcagc atcggattgg caaagtcaat ggcaacagca ccgcctactg
    11761 acgaagccat ctgatccatt aacccgcagg gcttctggaa atacacgttt tccgcatact
    11821 gccccatttg tgccagttct accggtgtga accgattttc ggcatacagg gcattgcaga
    11881 tatttcccag cagaacctca aaagctgctg atgaggacag accagagcct gccatgacct
    11941 gcgaggtggt gtatgcatca aaaccggttt ccagcggaca tccgtactgc ttgaagcggg
    12001 cagcgattcc gcggatcagt tccgctgctg tgggcttttt gtccgttacc ggttccagtt
    12061 cttccagaga gatctcatct tccggaaagc cctctgaatg cacccggatc gtggtatctt
    12121 cccgtcttgc cacaacagcg atcacgtcca gattgatgct tgctgccagt acgtgaccat
    12181 gctggtggtc ggtgtggttt ccgcccagtt ctgtccgtcc cggtgcagaa aaaatggcaa
    12241 tttcccgacc gctgccgtac aactgcacaa aactgtcaat ggcacacagc agccggttcc
    12301 gataagcttc cgccagatct tcaccgccgc caagtactgc cgcgatgtgc atgaattctt
    12361 ccgactgcag cagtctgcga gcctcctcaa ttgtcttcaa tatgatgccc ctttctcaaa
    12421 tcgctttttc gggtaactgc tgtgtaaaac ttagggaaac actgaataaa gcttgtgcgt
    12481 aagatgcgta gtcagatttt tcggcagatt gcctagaaat tccgcaggca gacttggtgt
    12541 ctggcaaggg atttctgggc aatatgacgg aaaatctgca agcagattgc gtacaaagcc
    12601 gtcaggcggg ctttattcag cgtttcctta gttcccggaa gcggcgatct gcgttcctac
    12661 aattctccac tattcagtat aaccgatttt tcacgatttt gcaagcattc tttgtgattt
    12721 tttctatctc aatacagaat tcgaaaaaag caaagcctca gccttgaaaa ttgaagcaaa
    12781 tcccgcctta cgcttgcaaa caaaaacccg atacgggaat gctcccgcat cgggtttttc
    12841 ttatgctgtc atagccttgt tgtcagaaac gcttacgcgt tcgaaatcag ggaatctgat
    12901 tattcagccc acatagagcc cttcaggttc ttcagatccg ggcaaaccgg attgtcccag
    12961 gtagtgctct tatgaccagc accaaccaga gcaacatagt atgcctggta gaagatgtca
    13021 gttgcttcca gcttgccgtc tgcagaattc tctcctgcct tgctttcggt atcaatatcc
    13081 gtcaggaaga aggacagttt ctccagattc tcatcctgtg cgtgctctgt gccgtccaga
    13141 agctttgcat ccttaatacc agcaccctgt cttgcaatgt agtagctact gtagtacata
    13201 tcagtagcct ctaccttacc ggacaagtca gtatcgccct ttacgccgat gtaaaccttt
    13261 gcagtagcaa ccttttcacc cttatacagg atatcgaggt cggtatctac atatgccttg
    13321 ccttcgctgt cgaacagtgc cttcgggctt tctgcaccaa aggtcagatc gtccatgacg
    13381 ctctcgccgt ttacagttgc agacttcaca agatcagcag cgttgaaatt acgtccgtca
    13441 atagacagat agaagtttgc atccggattt acttcgattt ccagttctgc ttctgcagtc
    13501 gtggtggtta cttctgtagt agtggtagct tcggtagaag ctgtcgtgtt ttctgtggta
    13561 gtggttgctt ctgtaacagt tgtcgtagct tctgtggtag ttgcttctgt agtagaagaa
    13621 acagctgccg gtgtggtagt agtttcttca gtggttgctt cagtagttgc ttccgtggtt
    13681 tcttctgtag tagttgttgt tgtggtagtt gtgctggtag ttgtcttctt gctgaagtcc
    13741 ggcaactctt ctgttgtaga agctgtggtc ttcttgctga agtccggcag ctcttctgtt
    13801 gtagcagttg tagttgcctt tgtagtagtt gtttcagatt ctgttacagt agtggttgtt
    13861 gcactggtgc tggtggttgt cggacctgca ctggtgctgg ttaccgtagc agtcgttgct
    13921 tcagtagtaa cagtcgttgc ttcggcagta gtggtagttg cttcggtagt cgttacaata
    13981 ggttcttcgg tagtggtggt tgtagcagca gtcgttgttg tagtggttgc tgcagttgtg
    14041 gtggtgacag tcgtagcctc tgtagcagtt gtagtctgcg gttcttctgt ggtagtagtc
    14101 gttgccgcag aggttgcggt tgtagtggtg gtagctgttg tagttgttgt agttgtagca
    14161 gttgttgttt cggtggtttc cactacatca ttcatgatta cgcggatcgc accgtccttc
    14221 agaccaacca tttcgtcgaa gatgtcttcg ccggttacgc cctcgccgtt caggtagttg
    14281 aaacgcttga catctaccat cgtggtgccg ctgggcagtc tgtcagaacc attcggtgcc
    14341 cagtctacag ggaactcata gtactcaaca gtcttgccat cgtattcacc agtctgaagt
    14401 gtcagaccat attcgtcagc cagtgcctgt acagtatcct tatccggcac attgaagatc
    14461 atgcggaata cctgattcat ctttgtcggg tcagttgcac cttcggcaga acgtgcacca
    14521 atgaaacgga tccagcggtc gctgccgtca gtaattttag aagtgccgtc cttgtatgca
    14581 atgaaatcat ttgcattcgg aataccgctg ccgaagtcgt tgacaacaaa tgctctcttt
    14641 ttcagcagct tggacggaat ggtgtacagc tttgcagttg cctccggcag gttgacaacg
    14701 cagttgatac catagctggt tgcgtctacg ccttccttgg cagttacctt cacgcccaga
    14761 gtgtagttct tcagagaagt ggcatccttt tcactgggat atacgaatac ttcgtcaatg
    14821 tccagattga tgtccttttc ttctgtggta gtggttgtgg tagtcgtgtc agttgtagtg
    14881 gtttcagtct gtgcatctgt gtaaacgcgg ataccgccgt tgtagtaaac tacatcagta
    14941 gtcgcaatat ctgtgccgtt ttcgtcgatg tactgacggt tcggagccag atagccgtcg
    15001 ccgtgtgtac cctcaaagtc ccagtttacc ttctgatact ctgcaaagtt taccgggaac
    15061 agatagtagc tgtcgccgtt ttcatctgtc tgcagttcca gaccatattc ctttgcgatg
    15121 tccactgcgt tttctgcaat ggtgcagttg atgtcgatca ccttgtcacc gctgatcgtg
    15181 atcgtgctgg tactggaagt ccactggaaa tacatccaac gcttggacgg ctcaacgctc
    15241 atatccttac cgtttgcctc atagcccttc aggttctgcg gagaaggagc caggtcgccg
    15301 ctgacataaa cgtccttata accatagtcc ttcttgttct tcgggttctt gtagatcgtc
    15361 aggatcttgg aagtaacttc cggaacttcc agagcgaagt tgaagccctg tgcgtccaca
    15421 tagttttcaa ccagaacgga cagcgggatc tttgcatagt tgcttgcagt cttgacctgt
    15481 gcttcgtcta ccttgaagat gatgcccttg cccttcggag cttccgttgt ggtggtgccg
    15541 atggacttgg tagtggttac aacagtggtg tctgcctttg tagcagtggt ctgtttttct
    15601 gtggttgttg cagcagatgc agaagtagct gctgcggttg cagtggtttt agctgcggtt
    15661 gcagaagttt ctgtagcctt gctggtttca gcagatgctt ctgtcgaagt agaagcagtg
    15721 gtttttgctg tagtgtcagc tgtggtagct acagttgtaa ctgtggtttc cttagtagtt
    15781 tccttggaag taacagtagt ttcttctgtc gttgcagaaa cggtgctttc ttctgtggtt
    15841 gcaactgttt cagtggttac agtggtcaca gttgtggaag tagtggtctg atccggatct
    15901 tcgtcgaagc acagtacgat cttgtcgatt gtcacatcgc cgaacttcgg ataataaacc
    15961 atgaactgta cagtttcacc aacagtcttc ggaatgtcat aagatacgat gacctgcttg
    16021 tttgccggta cagccagatc cttgaattcc gtgctctgcc attcttcacc ttccggcaga
    16081 ctgtcgttcc atgtgccaaa cgcaccgctg gtgttcttgt catttgtctt tacggtgtag
    16141 taagcaattg ccttgtatgc acccatggga tcgaattcaa ccttggtgtt gcttgccttg
    16201 ccgtcagtac ccttgtcttc ctctgccttc ggagtcagtt ccttggtgta ctttgcggaa
    16261 actgtagtgg tcggctgtgt ggtcacagtg gttgcagcag tcgtagtgat cgttgcatca
    16321 tctgtgtaaa tcacaacaga atctacagtg aagtcctcat aagtaccgtc gccccaggca
    16381 ccaaacttca gagaaccatc atattccttc tgaatggttg cgtccacagt cttggtgatg
    16441 tcccaggtga attcgccgga cttgcccaga gttttgtttt cctgtgtggt ctgtgtccaa
    16501 taatccggtg ctttctgtgt agaagaaccg aacatatagt tgtatgtgcc gatttcctta
    16561 tcagaggaaa cgttgacaac gaccttcttg acaacattgc tgttggtgat gccaaagtca
    16621 gcccacttcc agccgatcat gccttccttc gggtcagcct tctgtgcatc cttcagcagt
    16681 tcgctgtagg tcttgccgac agtgatcttt ttcacgccct tttcctctgc ggtagtcgct
    16741 gtagtgacag cagaggttgc agtggtttca ctggaagcaa cagtagtttc actggaagca
    16801 accgtggttt ccgcggtagt ttccgcagtg gtttcactgg tcgttgtagt agtggtgact
    16861 tcatttgcca gctctactgt gaagctaact gaaatcactt cgcctgctgc cggcatagcc
    16921 cagccagtgg aatcaattgc cagcttgttg caggtgaaat cccactcatc agatgcagac
    16981 tgtacagaag acggagtaaa tgtcgtatac tcctgattga gttcatgagt cgtttcgccg
    17041 atctttacag aatcaattgt gatcttctta tacagagatt taccgtcgta aagatccgga
    17101 tcctgaacgc acaagtcgaa gaaattgtag gaactcagag catcacccca gtcacctctt
    17161 tttacagaaa cttcataaga gccgctctta tcgatggtaa catcagttcc agttcctgcc
    17221 ttatagttgt tcagcgacca tttgccatcg ctactctgaa tgtgcagaaa cgcaatcggt
    17281 gcatcatctg ccgctgtcgc agcaagggac atcatcgggg tcaccaatgc actggtcaga
    17341 atggtcgttg ccgccattac gccagctgtc agagatgaaa gaaacttttt catcttcatg
    17401 attatcattc ttcctttctt gaattgatgc ggcgtattgc cgcttagttg tgtgcagaga
    17461 agcttttggc atttcaaaaa aacgaatgcc gccgcactgt ttttcacgtc cccctttccg
    17521 ctttcaaatt gtacagccgt gcgatttgtc ctctgaaatc cgcccagtgc tttcctgcac
    17581 cctacttctc catataccag catagcatat ttatcgttat tttgcaagta ttttttttat
    17641 tttccacata tttttataca gcaccaataa aagcacacct tgtttttgtg cattttttct
    17701 aaatctcgaa ttgccagtga aaaacaactt gataatttga tacgacatgc cggtgaattt
    17761 tgcatttttc aaatgtcaat ttccggatac attgtgaacg ccgtcacggg gtgcggcggc
    17821 gatctcctgc aaatcctgtt ccagctgact gtccagcata cggcagttgt tccggtcata
    17881 gaacacaccg gtaacatccc acagcatggg acacatacct ttggtatacg ccgcctgtgc
    17941 caccgcggtg aggaatgtcc ggacagtttc ttccgtcttg ttcttggtaa aacagccgta
    18001 ctccccgatg atcaccggca cgccgttatc cagaaaccgg cttttcagct tgtccatgtc
    18061 gttttccaga atcctctgat ctctgggtga accccatgtg gtcttggctt ttccccagtc
    18121 ggcatcttct tccagaatgc agaacgtagg cggcgtataa taatgtaccg aaaccgcaca
    18181 tcgtccggct tcgtcctgtg gcatctgaaa ggcttcatca caggtcaggt ctacgtcagt
    18241 ggcataaccg gcgatcagca gatgccgctt ggggttgttt cccctggaac cgcgtaccag
    18301 atccacaaac tgctggttga tgcggttgac aatgccgtat gcctccgcct taccatcatc
    18361 agagccgctg tagcggttcc acagatcgtc aaaacagcct tcttcgttca tggattccag
    18421 aatcaggtgg tcaccgtaat ctgcaaaccg gtctgtcacc tgctcccaga tacgggtata
    18481 tttttccata caggtgtcat agtccgtgga aaatcctgcg atccagccgt tgtcccagtg
    18541 gagatttacc accgcataca tgccgttgtc cagcacatac tgtgccaccg tttccacacg
    18601 gtctaacagt tccggtgcaa tggtgtagtc ctctgccatg agattcgacc acgccacagg
    18661 aatccgcacc gaatcaaaac cggcggcagc gtagccggca atcatatcct ctgtaattgt
    18721 cgggctgccc catgcagttt cgtagctgtt taccccgccg ctgctgtcga tccagtcgcc
    18781 gcaggcttcc agtgtgttgc ccagattgat gcccagcccc atttcttcca cgatctggct
    18841 ggtagtcatg tcgtcccgca tttcagtgcg ggggttgctg tcggcttcct ccttgctgct
    18901 ctcctcttta gaggaattgc cgcagctgca cgacaacacc gccagcaccc ctgccagcag
    18961 cagagaaacg actttttttc cgtttcgcat gtaacacctc ttattccttt ttcggcggct
    19021 tcatggtgag ggcgatgcgt ttccgcttca agtccacgtc cagcacccag actttcacca
    19081 cctcgccgac cttgacagcc tccagcgggt gcttgatgaa ccggtcacag atctgggaaa
    19141 tgtgcaccag tccgtcctca tgaacgccca cgtctacgaa acagccaaag tcaatgacat
    19201 tccgcacagt gcccaccagt tccatgcccg gtttcaggtc tttcaattcc atgatgtccc
    19261 cgctccggag cagcggcggc ggcagttcgt cacgtgggtc acgccccggc ttttccagtt
    19321 ccttgagaat gtcccgcaca gtgggttcgc ctacgcctgc ctttttcgca atggcagaaa
    19381 tgccgtgctg cttggcaatg gcaccgatct cgctgcggtt ctgtccggca atgtctgcca
    19441 gccggaagcc gcactcctgc aagatctgct ctgctgcccc gtagctttcc ggatgcactg
    19501 ctgtattgtc cagcgggttc ttggctccgg acacccgcag gaatccggca cactgctcga
    19561 atgccttcgg gcccagtttt gccacctttt tcagctgctt ccggtcggtg aacgccccgt
    19621 tttccgtccg gtatgtcacg atgttcttgg caatggtctg gttgatgccg gcaatgtggg
    19681 acagcagaga gaacgaagcc gtgttcaggt ctacgcccac atggttcacg cagtcctcca
    19741 ccacgccgtc cagtgcggca gacagttccg ccttgggcat gtcatgctga tactgtccta
    19801 cgccgatcgc ctgcggatcg atcttcacca gttccgccag cggatcctgt aaccgtctgg
    19861 caatggaaac ggcactccgg agagaaacgt catactccgg aaattcctcc gccgccagct
    19921 tggacgcaga atataccgaa gcccctgcct cggaaaccac catgtacgcc gcggaataag
    19981 gcagttcctt cagcagttcc gccacgaccg cttccgactc tctggaagcc gtgccattgc
    20041 cgatcgccac cgcggtcacg tggtgcttcc gcagcatctt tcggatcacg tccttttccc
    20101 gttccagcga agcccctttg ctgacggtga tatgtgccac gccggtgtcc agtaccttgc
    20161 cggtttcgtc caccactgcc agcttgcagc cggtgcgata gccggggtct acgcccagaa
    20221 cgatgcggtt tttcagcggt gccgccatga gcagctgccg cagattggac gagaacacgc
    20281 cgattgccgc cgttgccgcc tgctctgtca gagccttgcg ggtttcccgt tccaccgccg
    20341 gaaacagcag ccgctgaaat gcatcttccg cggcagtctg caccagtttg ccgcaggggc
    20401 tctggttctt cacgaacttc tgaatcagca gcgaagcccc gtgtccttcc ggcagcgtaa
    20461 ctgccacttt cagagcaccc tcccgttcgc cgcggtcaat ggcaaggatc cggtgcccca
    20521 caatgcgttt caccggctct gcatagtcgt agtagttctg gtacggcgta tccgcatctt
    20581 cctctgtggc acgggactgt atcgttccgg cagacatcac cagccgacgc atctgctttc
    20641 gcaggtcggc atcatttgcc atttcctccg ccagaatgtc cattgccccc tgcaatgccg
    20701 cttcggtgtc cggcacttcc tcctgcaaaa aggcttctgc ctcctgagcc ggattcgtcc
    20761 ggggattctg tgccatgagg attcgtgcca gcggttccag tccgcggaca atcgccatag
    20821 atgcccgtgt tttccgcttg ggacgatagg gcaggtacaa gtcctcggtt tccaccagtg
    20881 tttccgcacc gcggatcttt tcttccagtt ccggtgtcag cttgccctgt gcttcaatcg
    20941 ccgtgcagat ttcttctttc cgctgttcca gcttccgcag atattgcagc cggttgtcaa
    21001 tggcacgaag cacctgatcg tccagcgaac cggtacgctc tttccggtac cgtgcaataa
    21061 acgggatcgt cttgccgtcg tccagcaagg caatggtatt gtcgatctgt tcctgccgca
    21121 gttggaattc ggcagccagt ttctgaggaa tattcaaata gagttcctcc gtttctgtga
    21181 tttcaatcgg actgttataa tagtataaca aacttttgga aaaattgcaa gtgatcattt
    21241 tcacatgtcc ttacaaggac aatcccacca gacactcttt gtgcggcatt gtttgagaat
    21301 ctgaaaacca aaaaccgatt tctgcacctc cgcaaaaatc ggtttttctt ttattcatgt
    21361 tgtgcaaagc catcacgcgg ctttgtgtgg tattgcctca attgtgttat gcactgtgcg
    21421 gcaagaagct tacaatatgt accagaaatt gcagcagaac aaccgcatcg ttgctgctga
    21481 cctccttatc tccatcgcag tcagcatttt ttaccgcatt cttattcaaa gacaccgtgc
    21541 cggaacaata ccggttcagc aggatcacgt ctgtgatgtc cacttgcccg tccaggttga
    21601 tgtcgccgta gataggcgaa aaagactcag atgaggtaga agaagtggtg gtttgatttt
    21661 cagaagttgc agtagcttgg tgtttaattg cagttgaagg aggtgtaact gtccgtgccg
    21721 tagtactcgt tgcttttgac gttgatatag ttgttgttgt aacaagttca gttgttgtag
    21781 ttgccacagg ttctgtggtt tgaacaccac ctatggatac aaaagaaatt ttattttctg
    21841 ctgcagaagt ttctgctgca gaaccaaaat atccataaat tgttaagttg gtacaatttt
    21901 caaaagcact ttctccaata tccaccacac tatttggaat ggtgatttcc gtcagactgg
    21961 tacagccttt aaaagcatac cctccaatac tcgtcacgct gtccggaatc gtgattttgg
    22021 tcagtctggt acagtcagaa aaagtataat atccaatact cgtcacgcta ttcgggatcg
    22081 tgatttcggt cagactggta caaccctcaa aagtactctc gatacttgtc acgctgtccg
    22141 gaatcgtgat ttcagtcaga ctgatacagc cgtcaaaagc acgccctccg atactcgtca
    22201 cgctgtccgg gattgtgatt ttggtcaaac tgatacattc gtcaaaagca taccatccaa
    22261 tactcgtcac actgtccgga atcgtgattt cagtcagact gatacagccg tcaaaagccc
    22321 acactccgat actcgtcaca ccactgggta ttgagacaga gccagtacaa gttgttccat
    22381 caatcaaaat atgattaaca attaccaatg gattttctgc ttgttttatc tttaaccaag
    22441 gtgtatcaag aaaagcttta tatccaatat acgtcacact atctggtaac gtgatttcgg
    22501 tcagactggt acagccccaa aaagaacaat ctccaatact cgtcacgctg tccggaatcg
    22561 tgatttccgt cagactggta cagccgtaaa aagcatactc tccgatgctc gccacgctgt
    22621 ccgggatcgt gatttccgtc agactgaagc cggaaaaagc atgctttccg atgctcgtca
    22681 cgggctttcc atcgatttct gtgggaatat tcactactgt agcagcatct ttattgcatt
    22741 tggcgatttc gatttctcct gtatccgtca cattataatg caaaccttca tatgtgccat
    22801 cgtaagcact cgccgataac actgttccca cgctttcttg cacactctcc accccagaca
    22861 gccccacacc gctcacgcaa agcacgccta cggtcactga tgcaagaact tttttccaaa
    22921 acctcatcaa ctgcatcctc ctcaaattag tcaagattca acgtttttta cattatagct
    22981 caaaaagaaa aagaaaaaac aaccattttt gcgatatttt gtaaatttca gtatttcatt
    23041 cgcatttaag taacaaaaaa gccagcccct gcacatacag gaaccagctt tttgcaacat
    23101 tcacttgtca gcgttttact tctgcttcat ctccgcagaa actgcttcaa actcgtctac
    23161 gatctcctgt tccggagcct tggtcagcag cgaaaccaca acgatcgcga tcagtgcaca
    23221 caggaatgcc ggcagcagct cataaatggc aaacatgccg cccagcttac tgatggcaaa
    23281 tttccatacg aaaatcatca caccgccggt aatcagcccc gccagtgcac catacttgtt
    23341 ggaacgcttc cagaacagcg aggtcagcat taccggtccg aaagtcgcac cgaaacctgc
    23401 ccatgcaaag gaaaccacgc ggaatacaga actgttctga tcccacgcca gcacaatgcc
    23461 cagcactgca ataatcagca ggacggcacg tgctgcgatc atggactgct tttccgacag
    23521 cttaatatgg aacacgcctt tcagcaggtt ctccgacata ctggacgaag ccgccagcag
    23581 ctgcgagtcg gaagtagaca tggtgcacgc cagaatgcct gcgaagatca gtccggcgat
    23641 cagggcagct cccacgctgt gtttgctcaa taccgttgcc atttccagca caatggtttc
    23701 cgccttggaa gaggtttcaa gcggagcaac cgtgccgttt accgtcaggg cgttgccgat
    23761 gataccaatg aatactgcca cgcccattgc cagcactacc cagacagaag caatgcggcg
    23821 ggagatcttg accttgttct tgtcatcgat cgccatgaac cgcaggagaa tatgcggcat
    23881 gccaaagtaa cccagtcccc acgccagcgt ggaaatgata ctcagtccgc cgaacgaaac
    23941 ggcttcgccc tctgcggtgc gggtgctggt aaagctaagg taattttcaa tacccttggc
    24001 attttccaat acgttgtcca gtccgccggc agtatgtacg ccatagatca ccagcaccac
    24061 cagagccgaa gtcataacga tgctctggat caggtcggta aagcttgccg ccagaaagcc
    24121 gcccagacag gtgtaggaaa tgatgatgac cgcactgatg atcattgcca tgtgatagtt
    24181 ccagccgaac agctgattga acagcttgcc gcaggcggag aaacccgaag cggtatacgg
    24241 cacgaaaaat atcagaatga tgactgccgc aatgcccatc agcacgtgct tatcgtcccg
    24301 ataccggttg gaaaagaagt caggaatggt aatggagttc cggcaacgct gagaatacac
    24361 gcggaggcgt ttcgccacca gcagccagtt cagataggtg ccgatcgcca gaccaatggc
    24421 agtccagcct acctctgcac agccgttcag atacgccagc cccggcagac ccatgagcag
    24481 atagctgctc atgtccgaag cctctgcact catggcagaa accaccggtc cgagccgccg
    24541 gccgcccaga taaaaatcgc cgacactgct gttctttttg gaatacacag caccgatcac
    24601 gaccatcatg cccatataga caagaatagc aatcagaata catactacgg attttgtcac
    24661 aaatcatatc tcctttcaat ttggcgtaag agcctgttca ggcaataccg cacaaagatt
    24721 gtgcgacag
//
LOCUS       DAWBGR010000011        24140 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_771_length_24140_cov_4.312643, whole genome shotgun sequence.
ACCESSION   DAWBGR010000011 DAWBGR010000000
VERSION     DAWBGR010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 24140)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24140)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24140
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_771_length_24140_cov_4.312643"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4522..4531
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6835..6844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13875..13884
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6065 a         5955 c         6318 g         5772 t
ORIGIN      
        1 tcgctttgca tagattttct tttgaatctt tgtctaactt tttggggtca gttcatattt
       61 tcccgtaccg gttttatcat gctaaaagtt taggcaacac cgcacaaagc ctgccttata
      121 tcaaagcatc gtatgatctt ccgctgtatc actttgctgc aatacctcgc ttgccagata
      181 cagagagccg cagatgacga ccatgccgcc gtctgccttt gcctgtgcca gtgccgtttc
      241 atatgcctgc tgcaatgacg gagcagtgtg cacttccgcg tggttttcga agcatgccgc
      301 cagtgtttca cgcgggacgg cgtgatgtac aaaatcgtcc acacatgtga ctgtatccag
      361 cacatcaccc agtatccggc aggcagtggc atagtccttg gtggagatca tgccgcagat
      421 gccgtgtatc ggcttggtgc ctgcgtgttt cagcagttcc gccagtgcca tgacacctgc
      481 ctgattgtgc ccgccgtcca gcagtactgt gggatttttc cgaagcactt ccgttctcgc
      541 ggggacacgc accttggaaa acgcctcgtt tgccgttgcc gccgtaatca gataaccgtg
      601 catgcccaga aaccgaatca cttcaatggc agtcatggcg ttatacacct gatgccgtcc
      661 ggacatgtgc agcgtatagg tgaagctgtg atactggaac acaatttcct ccagcgtttc
      721 cgacacaatg cggcagtcct gcatgttggg aatcaccagc tgggcatttt tccgcttggc
      781 agtgtcctgt accagacaca ccacgtccat gggattgtcc gtggagagca ccacagggca
      841 gcccgacttg atgataccgg ctttctgggc ggcaatttcc gtcagggtgt tccccagcag
      901 tgccatatgg tcaaaggaaa ctgaagtgat cgcacaaacc agcggcttct ttatcacatt
      961 ggtagcgtcc agcaggccgc cgatgccggt ttccagcacc acaaggtcgc acttctgttc
     1021 cacgaaccag agcagagcaa tggcaaacgt gatctcgaac tgcgagcagt cctgcgatac
     1081 cggacacgcc tgcacctgtt cacagaaccg gcacagtgcc tccggcggaa tatccctgcc
     1141 gtcgatctga atgcggtcgg tatagtgccg gataaagggt gaggtcagtg tgcctgtacg
     1201 aaaaccggca gccgttgccg cccccgcaca aaatgccgtc acagaaccct ttccgttggt
     1261 tccggcaata tgcaccacct gcaactgctc ctgcggattg ccgagggctt ccagcagagc
     1321 ctgaatgcgg gacaagtcat gcactggctt gccgcttttc tgatacccct gaatataggt
     1381 cattgcttcg gaaaacgtca ttgcactcac tccctcatac gttcacatac ctgcaatcat
     1441 tactgcaacg ctgccataga ctgcacgatc ttgtccattt tctcctgtgc ctttgccagc
     1501 tttgcctttt ctgcttccac cagcttttcc ggagcctttg ccaggaagcc ctgattggac
     1561 agcttcttgc tcaggaagtc cagatccttc tgtacctttt cctgctcctt tttcagccgt
     1621 gccagttcct tgtccttgtc aatgagttcg tccatgggga taaagatgcg gacactgtcc
     1681 gtgactgcgg tggcagcgtc cggcatgtcg aacttttcgc ccacttccac agcggaggca
     1741 gaagccagct tctcgaagaa taccgcggtg ctccggtaca gttccggctc ggtcgtttcg
     1801 atgcagacac gtgctttctt ggacggcggc acctgcattt cggtacggcg gttccggatc
     1861 gcacgaatgg catcgatgac attttcgaac tgtactgcat cggcaaagtc atacttggca
     1921 tcgtagaccg gataggattc cacaataatc atggattcgc cgtcagtcag cacctgccag
     1981 atctcctcgg tgatatacgg catgaacggg tgcagcagct tcagcatgcc acgcattacc
     2041 cataccagaa cagcctgtgc cttttccatg gcagcaccgc cagcctgaat ccgcggcttg
     2101 gtcagttcga tgtaccagtc gcagtaaacg tcccagataa agtcgtagat cttggcactt
     2161 gccacaccga tctcaaactt atccagattc tcgcccactt ccttggcaac ggcgttgaac
     2221 ttggacacga tccagcggtc ttccagctcc agatcttccg gcagtccgtg gtactggaaa
     2281 tcctccggca gattcatcat gataaaacgg gaagcattcc acagcttgtt ggcaaaattg
     2341 cgggcagcct tgaccttatc gtcactgtaa cgcatatcgt ttccgggagc attaccggtc
     2401 gccagcataa accgcagagc atcggcaccg aattcgtcaa tgaccttcag cgggtcgata
     2461 ccgttgccca gcgacttgga catctttctg ccctgctcgt cgcgaaccag accgtggatc
     2521 agaacagtat cgaaaggcac ttcgcccatg ttcttcagac cagagaacat catgcgaact
     2581 acccagaaga agatgatgtc ataaccggtg accagtgtat tggtgggata gaagtatttc
     2641 agttcctcgg tgttgtccgg ccagcccagt gtggagaacg gccacagtgc ggaactgaac
     2701 caggtgtcca gtgtatccgg atcctgccgc atgggcttgc cgcacttcgg gcagactgca
     2761 ctgttttcct tggtaacaac catttcgccg cagtcgtcac agtagaacgc cggaatctgg
     2821 tgtccccacc acagctgacg ggaaatacac cagtccttga tattttccag ccagtggaaa
     2881 tagatcttgt tgaaccgttc cggaacgaac ttggtcttgc cgttcttgac agcgtcgatc
     2941 gccggctgtg ccagtggctt catcttgaca aaccactgct tggaaacccg cggttctacc
     3001 gtggtgccgc agcgatagca ggtacctaca ttgtggctgt agtcctcgat ctctaccagt
     3061 gcaccctctg ctttcagatc ttcaacaatt gcctttcttg cctcataacg atccatgccg
     3121 gcatacttcg gatatgcgtc cacgatcttt gcatcatcgg tcatcacgtt gataacttcc
     3181 agattgtgcc gcagccccac ttcaaagtcg ttcgggtcgt gtgccggtgt gatctttact
     3241 acgccggttc cgaagtccat ttccacataa tcgtctgcca caatggggat ctcacggtgt
     3301 accagcggca ggatcagtgt ctttcccacc agatctttgt agcgttcatc gttggggttg
     3361 actgctacgg cagtatcgcc cagcagggtt tccggacggg tggtcgccag ttccagatag
     3421 ccgctgccgt ccttcagcgg ataccgcaga tgccagaaat gccctgcctg cacttcgtat
     3481 tctacttctg catcggaaat ggaagtcaga cacttcgggc accagttgat gatacgtttg
     3541 ccgcggtaga tcagaccttc ctcgtagtac ttgataaata cttccttgac tgccttgctg
     3601 cagccctcgt ccatggtaaa gcgttctctt gtccagtcgc aggaggagcc cagctttttc
     3661 agctgttcca cgatcctgcc gccgtactgc ttcttccatg cccaggcacg ttccagaaaa
     3721 gcttctctgc cgatgtcctc ttttttgata ccctctttcc gcatggcttc cacgatcttg
     3781 gcttcggtcg caatggaagc gtggtcggta cccggcagcc acagggcact gtagccggac
     3841 atccgcttcc agcgaatcag aatatcctgc aaagtttcgt ccagtgcatg acccatgtgc
     3901 agctgtccgg tgatgttcgg cggcgggatc acgatggtat agggctgctt ctcagagtcc
     3961 acatggggcg tgaagcttcc gctgtcgttc cacattttat agatgcggtc ttcaaagtct
     4021 ttcggctggt aagatttcgc cagttcctgt ttcataaagt tcgttccttt ccatttcgat
     4081 caccgataaa aaaagccccg gcaatcctca gaattgctca gggcgaatca gccgggaaat
     4141 tttccgtctg ctgtccgtgt taccacctga attcggcaac gatcttgccg cactcatcct
     4201 gcacggttac gggtgcgaac cggcacagac tacccatttc cgtcctcacg gaaacttccc
     4261 ctgtgctgct ccgaggctac cttctcccac actgtccgaa aacttacacc gaccgttttc
     4321 tctctgtcag aaccagcggc gggatactcc tcctcttctc tgcatttctt gttttcttta
     4381 ggaaacactg aataaagctt gtgcgtaaga tgcgtagtca gatttttcgg cagattgcat
     4441 agaaattccg caggcagact tggtgtctgg caagggattt ctggacaata tgacggaaaa
     4501 tctgcaagca gattgcgtgc annnnnnnnn ngagaagcag tccataagct tcatggtttc
     4561 caagcccaag taccggcttt cggatctgat cttgtcggag agcaacttgg acgaattgca
     4621 gacggtcatc aaagcgaaag aatactggaa caaggtattt gtggactgga atctgcaatc
     4681 cgtgatgaaa cagcggaaaa gcctgttcat caacctttac ggagagccgg gaaccggaaa
     4741 gaccatggct gcccatgcaa ttgccaacgc tctgcacaag gaaatgatct gcgtcaatta
     4801 tgcggatatt gaatccaagt atgtgggcga aaccagcaag aacctgacaa atctgttcca
     4861 cactgcctca gaaaaagaca tcatcatttt ctttgacgaa gcagatgcac tgctgagcaa
     4921 gcgtgtgacc aatatgtcca gttccacaga tgtcagcgtg aaccagaccc gttcggtgct
     4981 gctgacactg ctgaatgact atgagggcat ggtgatattt gccaccaact ttatcagcaa
     5041 ctacgatccc gcatttatgc ggtggatcca gtaccatatc cggtttgatc tgccggacaa
     5101 gtctttgcgg gaaaagctgt ggcggctgta cattccgcag gaaatgccga cagaccttcg
     5161 tatttccgag attgccgaaa aggatgacgg cgtaagcggc agcgatattt ccaacgcggt
     5221 gctgaaagct gccctgaaag cggcgaagaa tcaggaatcg tttgtgaagc aggcggactt
     5281 tgaagatgcc gttgcggaga tcgtcaagag caaaaaggcg aacaaaagcg gaagccccag
     5341 agatgttaag attacaaaat ccgagatagt tccggaatcg gaagtaccga aagatattgt
     5401 aaataaatca aaagaatttc aggaggtgaa ctaaaatgat attgttttca gtacttgctg
     5461 cagtctgagc ctgtgtggtt ctgacactgt tggacgcatt ttacgacagt atcatcaaat
     5521 ggctgtccaa gatcggtgag gttgcacagc gggtggtcaa gggtgtactg atcggctgca
     5581 aaacatttct ttctgacgtg cggaagaata tcgggaagga gatttccaag aactattcca
     5641 aggtcggtga aaaatggcag gtgacagagg tttccagaga aattcctgcg gacaagattc
     5701 cggacgagat ctatgacaga atggaaatgg aagacagcga ttctctggaa ctgacagaag
     5761 acatcgaagc acaacttttg agcaactatc agtgaggtga agaaaatggc tggatttttt
     5821 gaacgactgg caaacatgat cgatgaggaa gtcagcaagg caggtgcaaa cgggaacacc
     5881 tcgtctgtcc ctgcggctgc cccgaagaag cctgtcacat ggaacgaatt cctcgagcag
     5941 acaggtaagg acgtagatca gcaggtcatc agtcacgccc gaaaagaggg cttgatctac
     6001 gtgggcggca agtgcaaatt tacggcgaca aagtgtgagg aagacaaaga caaggaaaca
     6061 tttgttgtca acgtagacgc tgcactctac ttcaaagatc aattcaagac ggaaaagaat
     6121 tttcagctgt atccgctgca ccgcgaggtg aagttcagcg aatttacaga agatgcggaa
     6181 acgcgggaac aactggaaaa gatcaagacc gaagtcgtag aactagatgt ggaaacaccg
     6241 cagatcacgg cacaggagga gtaagctatg ggctttttta gtaaactggg agaaaaaatc
     6301 gataagggaa tctcgtggat tggtcagcaa ctgaaaaaat ttgcgacgct tgctgcggat
     6361 tttttcagac gtgtaaaaga cggaatccgt aagtttgcaa tgattatcgg caaattcttc
     6421 tcgcttctgg caaagtggct ggatactgct attaaatacg tagaagctac attccatgta
     6481 atcgtttctg gtgtacagac atttttgaag aaggtaggaa acgagtatca acagttgtcc
     6541 tataactata cccgacaaga tgacggtacg tttttgaaaa acaccgtgat aaagcagacg
     6601 tttgtgtcag aagatgagat accggcagac atcagagagc aggcactcaa gctggcagac
     6661 ggcaaaatgg tggatattac agaaactgcg gcagaaagag aagaagaagc atgcacgcag
     6721 ctgggtctga gtgcgtaagg gggaatgatc gatgccgttt attatcattg gtatcattat
     6781 cgctctggtt ttgattttcg gcggaaaggc cattgccaaa aaggcaggtg cactnnnnnn
     6841 nnnntcgtgg tacttggtta tgcgtacatg ttttacatgt cccttgttgt aggttggttt
     6901 gcagacacca gcgttttttt gatgttgttg ttctgcgtgc ttcctgttgc ggtatggttc
     6961 attcgcagaa agctgacatt cggaagcaga gtgaacaagc agatcaccaa ttacatcaat
     7021 tttgttttga atgaagatac gacaggaaaa accattctgt tgagtgactt cttggctctg
     7081 gataccacca aaaagctgat gaatgaacgg aaacaggtag acgataagcc ggtttcgcag
     7141 tacatcagcc agcagttcga tcagatcttc tacagcacgc tccgcagcag aatccaagag
     7201 gacaaaattg accagaacga aagaatttac ttttactggg aatactataa gtttctcgat
     7261 tatctggaat gctgcaacgt agaacttgcc gattacattg acaaggtcag tgccgtagat
     7321 atgagttata tttatgtttc tggtctggca ttcttcagca gcaagattct ggacaagatc
     7381 acggaaagct tcctgccctt tgtagagtcg gttccggaac tgaaagatgc tccgttctcg
     7441 acgtatacac tgaaaaacaa agcgtatcag gtgggctttc tggatccgta tataaaggaa
     7501 tccacattgc aggcttttga tgatgatccg gatttgtaca aagacgattt ttccgatctg
     7561 tatcacaaca tactggattt tgagatcgga aactgcattg agcagaaaga aatcgttcac
     7621 tccacaatgc atgcacaaga agatgatgac gacgaagaat atttcgaact gaaccacgca
     7681 agtccggacg gtattgaaga atacggaagc gagatcgagg cacatagaat tgccatggaa
     7741 gatgatgagg acgacctcga tatgctggaa ggctgtgccg cataaaaatc cgttttgtac
     7801 aggctcttaa aaaccaaaaa tcccagtcgc aaacaaaatg cggctgggat tttttttggt
     7861 gacagcaccg tataaagccg tcaggctagg aaaatgtata ccacttttgc agcttttccc
     7921 gataggcgtt gacgatcggg ttttgattcg ggttgtcaag gagaaaccga taaaggtctt
     7981 cttttgtgat cacatggata tgcgggctgt tcttgcccga atcagtaact gcaatgccgg
     8041 aaaaaagata gattttccag taggtatccg tatcggaact gtaatcgtca gcgtggattt
     8101 cttcctgatt cttgacctga caggtgattc ttctgatttt tccgctttcc ttttccatgc
     8161 ccagcagctc gaattcatac ttcggcgtgt ttcgtttgca gctggagggg agcaggcaga
     8221 agttcgattt cttgttgttc tcgttattct cgtcgttctt gttgtcctcg ttgttcttat
     8281 cgagaatcca catggctgta aggtcttcca gttcatcggg attcatagcc ttgcagaaat
     8341 tctcggcgtg aatcggaatc ttttcgtatg tcgtctgtgt cgcaacactg tacaagatgt
     8401 ccgccagttt ttgcaagtcc gtgtccgcga ttcgtctgat ggtctgctgc cgccgctggg
     8461 aaaaccgccc gactactgcc gacggcacat ctcaaatcgg ataccacttt tccacacggc
     8521 atgcccatga caacggatta gacggcattt ccgcttcttt ggcgttatgg tatgtttcaa
     8581 aatcaacagt atcttcggtc agccactgtg cggttgtttt cacttttgca atataatatt
     8641 tggcgtttcg atctcggaaa agtacaagat ctcctgcctg aatgttctga taggcgtgat
     8701 atgctgtttt ggaaacattc ccagagccgg tctccagcca tttctgctga tattcttcat
     8761 agtcgggcat gggttcaccg gcgggatagg cgttatcatc gatattccaa cccatgccga
     8821 aaaggttgtt ccggatacag tccttctgaa aattcagaac aatgttttcg tttcgcttct
     8881 tcggtgagaa aggtttgatt tgaatgatcc agtactccat aaaagttctc ctgttctgtg
     8941 ttctcttgga tatatcttta tgttaccatg tgatttgtga aaattctaca tggaaattgt
     9001 aaaatcagag atttgcagaa catctctata agaaatcatt ctcacatgaa aagaataata
     9061 gcacaatttc ttagaaaata gcatcatctc taattgattt tttccagata tgtgataaaa
     9121 tataggtgtg attaatgata caacaggctg gagaagcgaa tgtgaggtat gctttctcga
     9181 aaataaggga aacactgcac aaagcttgca agcagattgc gtgcaaagcc gacaggcagg
     9241 atttatgcag tgtttctata agagggaatg gagaacagag aacggctctc aagtgggaga
     9301 ggctgttata gttcggagtg atgccgcagg gacaattgca gttgtctttg cggctttgct
     9361 ttttgcttgc aaggctgttt tcgacaaggg aacagctgtt tctggctgac ttgcactttt
     9421 cttctcacag caaaaggaaa cggaactttc aaaaatcaag tgcagtggaa tttctgaaaa
     9481 aatctgcggc attttgtttt ttataaaaga aaaaaatcgg cgtttttctt gcttttttcg
     9541 gaaaaatcga aaaaaaccta ttgacaaagc cggcggaatg gtctataatg aatattagca
     9601 aaagcgatta gaggatcgca aaatgaagga aagcaatgac gctttatgga aagaaacagg
     9661 acgtttcttt tcataaggcg tttttttgtt attttgcaca tcttgcaaag gagaagtgac
     9721 tatggataac tacagaaacc aagagccgtt tgcaaagaac ggcttgtatg accctcgttt
     9781 tgaacacgag aactgcggta tcggtgcggt tgtcaacatg gacggcacat cggaccggaa
     9841 agtggtggac agtgcactcc gaattgtaga aacactggag cacagagccg gtaaagatgc
     9901 cgagggcaag acgggtgacg gcgtaggcat tctgctgcag atctctcaca cttatttttc
     9961 gaaagtggca aaagaagccg gaatccagat cggcggcaaa cgggaatacg gtatcgccat
    10021 gttcttcttc ccgcagacca aggaaaaata cgaaaaggca atgcagactt ttgaggaagt
    10081 gctgaaagag gaacatctgg aatttctggg ctggcgtgaa gtaccggtag atccggacgt
    10141 tctgggcacc aaggctctgg acagcatgcc gcacatcatg caggcattca tcaagaagcc
    10201 agacgactgt gaaaagggtc tggaattcga ccggaagctg tacatcgccc gcatggtatt
    10261 cgaaaagcgg gacagcgaaa cctatgtggt atcctgctcc agccgtacca ttgtatacaa
    10321 aggtatgttt ttggtcaaac agctgcgtct gttctaccac gacctgaata gtccggatta
    10381 tcattcggcg atcggtatcg tacactcccg tttcagcacc aacaccaacc ccagctggca
    10441 gcgttcacac ccgaaccgca tgatggtgca caacggtgag atcaacacca ttaccggtaa
    10501 cgtggataag atgctgagcc gtgaaaatat tctccactgc aaggcactgg acgaccgcat
    10561 gaaggatatt ttcccgatgc tggacgttcg gggttccgac tctgcccgtc tggacaatac
    10621 cttggaattc atgaccatgt ccggcatgga tctgccgctg gcagtgatga tgaccattcc
    10681 ggaaccgtgg cagcatatcc ccaccatgag ccgtgaaaag cgtgcgttct atcagtacta
    10741 cgcaaccatg atggaaccgt gggacggtcc ggcttcgatc attttctccg acggcgacgt
    10801 catgggtgca gtactggaca gaaacggtct gcgtccgtcc cgttattatg tgaccgatga
    10861 cggatgtctg atcttgtcct cggaggtcgg tgcactggac gatattccgg cagaaaagat
    10921 cgtcaagaaa gaaagactgc acccaggaaa gatgttgctg gtggataccg tcaaaggcaa
    10981 gctgatcgac gacgaaacca ttaagaattt ctatgcaaag cgtcagcctt acggcgaatg
    11041 gctggatcac aacctgttca gcctgagcga actgaaagtt cccaacaagc gtccgttccg
    11101 tcatggcgga aaggaactgc tccggttaca gcgtgcattc ggctatactt atgagtccct
    11161 gtatcatgtt atggtgccta tggcactgaa cggcggagaa ccgacttcgg caatgggtgc
    11221 agataccccg attcctgcaa tgtccaagaa gaatccgccg ttgtttgatt acttcaagca
    11281 gatgttcgca caggtcacca acccgccgat cgactccatt cgggaatctg tcatcaccga
    11341 taccaccgtt tatctgggcg tatccggcaa cattctggaa gaagacgaac ggaactgccg
    11401 tgtactgaaa gtcaacaacc cgattctcac caatctggat atgatgaaga tccgcggcat
    11461 ggacattgac ggactgaaaa ccgcagacat ttccatgctg tataccaagg acgtttctct
    11521 ccatgatgcc attgaaaacc tgtatcagca ggcagaggaa gcacacgcca agggtgcaac
    11581 gattctgatt ctgaccgacc gtggtgtcga tcacgaccac ttggcgatcc cgtcgctgct
    11641 ggcagtggct gcactggaaa cctatctggt acgtaccaga aagaatactg caatttctct
    11701 gatcatcgaa actgcagagc cttgtgaagt acatcatttc gcaaccctgc tgggcttcgg
    11761 tgcaagtgca gtcaatccgt atctggcatt ggattccctg tatgacatga tcaaccgcgg
    11821 tctggtggat aaggactatt cgcaggcaag caaggcatat attgacgcac tggtttccgg
    11881 tatcgtcaag atcgcttcga aaatgggtat ctccaccatt cagtcctatc agggctccaa
    11941 aattttcgag gcactgggca tcagcgagag cgttatgaac gagtattttc cggatacggt
    12001 cagccgcgtg ggcggtatcg atctggacga tattgcaaaa cgcagcatga ccatgcacga
    12061 gagtgccttt gaccccaacg atctgggcat ggacgaggga cttcgcagcg tgggctggca
    12121 caaggaacgg agcaatcagg aagaacacct gtacaatccg gaaaccattc acttgctcca
    12181 gcaggcaaca tggcgggacg attacaatct gttcaagcag tataccgcct gtgtcaatgc
    12241 cgaggaccgg cacattacgc tccgcagcac gctggacttc aagttcgcag aggacggcgg
    12301 cattcctatg gaggaagtgg aaagcgtaga atccattatg cgtcgcttta agacgggtgc
    12361 gatgtcttac ggttccattt cgcaggaagc ccacgagtgc atggcaattg ccatgaaccg
    12421 tatcggcggc aagtccaaca gcggtgaggg tggcgaagat ctggaacgta ttgtcaccgc
    12481 aggcagtgct gtcgataagt gctcggcaat caagcaggtg gcttccggac gtttcggcgt
    12541 tacctcgaag tatctgacct ccgccaagga gatccagatc aaaatggcac agggtgcaaa
    12601 gcccggtgag ggcggacatc tgccggcaaa gaaagtgtac ccatggatcg ccaagaccag
    12661 acattccaca ccgggtgtgg ctctgatctc accgccgccg caccacgata tttactctat
    12721 cgaagatctg gcacagctga tctacgacct gaaaaatgcc aacaaaaagg ctcgcatctc
    12781 cgtgaagctg gtttccgaag ccggtgtcgg caccattgca gccggcgttg ccaaggctgg
    12841 tgcaggggta atcctgattt ccggttatga cggcggtacc ggtgcggctc cgggcagctc
    12901 cattcacaat gcaggtctgc cgtgggaact gggtctggca gaaacacacc agacgctgat
    12961 ccagaacaat ctgcgttcca aggtcgtcat tgaggcagac ggaaaaatga tgagcggacg
    13021 tgacgtggtc attgccgcaa tgctgggtgc agaagaatat ggcttcgcaa cggctccgct
    13081 ggtaacaatg ggctgcgtga tgatgcgtgt ctgcaatctg gatacctgtc cggtaggcgt
    13141 tgccacacag aatccggaac tgcggaagcg tttccgcggc aagccggaat atgtcatcaa
    13201 cttcatgcgg tttatggcac aggaaatgcg ggaatacatg gcaaagctgg gaatccacac
    13261 cgtagatgaa ctggtgggac gttctgatct gctggtacag aagcagtatc cggcaggcac
    13321 gcacgagagc aagatcgata tgtctaatat cctcaccaat ccctatgcca aggaagaaag
    13381 cgatgtcaag atgcacttcg tgccggaaga cgttttcgat tttaagctgg ataagaccat
    13441 cgatgaaacc gtgatcattc cggaaatgaa agaagcactg gcaaagaagc agaagaaaga
    13501 gatccgtatc aatgtcagca acctgaaccg tgcactgggc acgctgttcg gggcagagat
    13561 cacacggaaa tattataata cactggaaga cgacaccttt gtggtacact gtaccggtgc
    13621 cggcggacag agcttcggtg cgttcattcc gaagggcctg acactggctc tggaagggga
    13681 cagcaacgac tatttcggca agggcttgtc cggcggtaag ctggcagtgt tcccgcccaa
    13741 gggttccaag ttcaaggaag acgaaaacat catcatcggc aacgtggcac tgttcggtgc
    13801 gaccagcggt aaggcattca tcaacggcgt ggcaggcgaa cgcttctgcg tccgcaactc
    13861 cggtgcttct gtcgnnnnnn nnnntgtcgt agtagaaggt gtcggtgaac acggctgtga
    13921 atacatgacc ggcggttgtg cagtcattct gggcgaagtg ggcaagaact ttgccgcagg
    13981 tatgagcggc ggtattgcct atgtactgga tcgggaaaat gacttgtaca ccaagctgaa
    14041 caagtcgctg gtaggcttcg gtgcagttac caagccggaa gatgtggcac agctgaaagg
    14101 tctgatcgaa gaacacgtgg catggactga ttccaagctg ggcaagaaga ttctgaagaa
    14161 tttcgacacc tgcctgccgc acttcaagaa gatcgtaccc catgattatg caaagatgct
    14221 ggacagcatt gcagaatttg aggcagaggg catgagccgt gaagaagcac agatcgaggc
    14281 attttatgcc aacacgagaa attaacagga ggagctatca tgggaaaatc aactggattt
    14341 ctcgaatatg agagagtcga aaatgctgga caggaaccgc tggaacgaat taagacctat
    14401 cgggaatttc ataccccgct ttccgacgaa gaacgccgga aacagggagc ccgctgtatg
    14461 gactgcggtg tcccgttctg tcaggcaggc aagccgatct ttggcatggt ttccggctgc
    14521 ccgctgaata acctttgtcc ggagtggaac gacatggtct acaacggctt ctgggaagaa
    14581 gctgcggacc ggctgctgct gaccaactgt tttccggaat tcacctcccg cgtttgtccg
    14641 gcactgtgtg aaaaggcatg tacctgcggt ctgaacggcg atccagtcac cgtcaaggaa
    14701 aacgaatacg ccattatcga aaaggcattt gccagcgggc acatcaaggc aaatccgccg
    14761 aaagtccgca ccggcaagcg tgtggcagtg gtcggttccg gtccgtccgg tctggcggca
    14821 gcattgcagc tgaacaagcg tggacatcat gtgacagtat tcgaacggga agaccgtgta
    14881 ggcggtctgc tgatgtacgg aatcccgaac atgaagctgg aaaaaactgt agtacagcgg
    14941 cgtgtggaca tgatgcagga agagggcgta gagttccgta ccagcgtaga tgtgggcaag
    15001 gacatttctg cggagcagct gctgaaagag tatgatgcag tcattctggc gtgtggttct
    15061 tcgaatccga gaaacatcaa tgcagacggt cgggacgcag acggcattta ctatgcggtg
    15121 gatttcctga aagccaccac aaagagcctg atcaactcca acctgtctga cggttactat
    15181 atcagtgcca agtataagaa tgtcatcgtc atcggcggcg gcgatacggg taacgactgc
    15241 gtcggtactg ccattcgcca cggctgcaag tctgtggtac agctggaaat gatgccgaag
    15301 ctgccggaca ctcgtgccga aagcaatccg tggccggagt ggccccgtgt ctgcaagaca
    15361 gactacggtc aggaagaagc cattgccgta tttggtcacg atccgcgaat ctaccagaca
    15421 acggtaaagc ggttcatcaa ggacgacaag aatcacgttc gtgcagtgga aaccattaag
    15481 ctgcaatctg tacacgatcc caagaccggc agaatgcgaa tggaagaaat cgagggcagc
    15541 gaacagattc tggacgcaga tctggtgctg atcgccgcag gatttctggg ggcacaggca
    15601 tatgtgacag atgcattcgg cgtggaaacc acaccgcgaa ccaatgtttc caccaagatc
    15661 ggtcactaca gcaccagcgt ggaaaaggtg ttcaccgcag gcgatatgca ccgcggacag
    15721 tcgctggtag tctgggcaat tcgtgagggc agagaagctg ccaaggaagt agaccagtac
    15781 ctcatgggct acaccaatct gaattgatgt tatggaaaca ctgcatacag tctgcccgcg
    15841 gaatttttgt acaatctacc gaaaaatctg actacgcatc ttacgcacaa gctttatgca
    15901 gtgcttcctt attattctga caattccaag ttattcatat accctttgca aaacaggctg
    15961 catttccgga aacggaggtg cagcctgttt ttcttgggaa acactgcatt ctatcatgac
    16021 atggaaaacc agagctcaaa aaaacagaaa aactgctctt atctgagtcc gcaacatatt
    16081 ccgtgccctt taacgcagca atcaggcggt tgatcatgtc cgctgccgga atcatcaatt
    16141 ccttgctgac cggaagctga atgactgtgc cgtcagtttt gtgaaagacg atataggtga
    16201 gcatcagttt cttcaccggc tcgcattggg aaatggcgtt gaactcggtg aacttttctt
    16261 ttgtggtgta gattcttttg ttggtgatga acagcgtttt gccgtcgccg acgatgtcca
    16321 aaactctggc gtaatggaaa tagccgagga ttacttcgcc ggatgccagc ttgtcctctg
    16381 catagttctg aacggtgaag tccgcatccc gttccagctt gccgccgttg atctttttgc
    16441 attcttcctc aaagtcgatg taatcttcgc cttcttcata gaaagcatag tttttgtcac
    16501 cgtcggtggc atcgtccttt aagtgcaggt tccggataac gtcgtgatat tctgccacat
    16561 gcagtctgag gaatcttctt ctcaaaatca agatgcagtt ttcatcgtca acatcgcttt
    16621 gctctccggt tgcaaacgtg ttgtttccgt tttggatgga cttccagttt tctgtgattt
    16681 cctcgaggga catttcgtcg gcgatccgcc ccagttcgag aaaatgctgc cgaaagagaa
    16741 tgcttttggc ggctttggaa tcgctgtagt ggaagctttt ttcctttgcg aaaatctgct
    16801 ctttcagttc cagtgtggat tctagtgtcc cgatatcgcc gtccttgtaa attttcatca
    16861 ggatactttc aatatgagat gtcacatcaa tatccatgat tttggcaagc ttttgcagtt
    16921 cgccgttttc atcgctgaaa aagtccagaa tcgctgtgta aaagtcgctc ggatagggat
    16981 ccagagccag tgcttctgtc aggttttcca ttcgcgtatc cggttcgcag tcctcggcac
    17041 tggcttcggt gtacagtttc attgcctttt ttgtatcttc tgccggaatc ggctcatatg
    17101 tgcacaggcc agcctcctga aacagctcca ttttttgaga gaacatatgg aatacatctt
    17161 ctcccacacc ctttttcaga gcacgctgta cattggcatt ggaaaagact ctgttggaga
    17221 tttttgttgc tgcatcagaa aaagcggata tgctcttggg cttgccgtcc tcgtccagtt
    17281 tgatgagaga actgaacatg gtactgtatg cgtatgtaaa gaagcacttg ttcagcggtc
    17341 tgatcagact gtcatacaaa gccttgtctg cttcggtgtg taccagatca tgttcttcct
    17401 gcatggcaca ttgttccatg ccctgaacga atggcagaac ggcatcgtgc acgctgaaaa
    17461 aagtggcttt cgattccgaa attttcttcg cggcaccatt cagatatttt gccacattgc
    17521 tggtggcgaa agtcttgttc agcattgctt cgatgctgta attttccgta tctacaccct
    17581 gttcctgaag ctcgtggaga atgttttccc cttcgttggt gatgacctgc atgtattccg
    17641 caatgtagct gtttctgatg tcgatgaagt gcttgaacaa gccgtagtcc agtatttcat
    17701 ttttcacttc gttgaaaata gcaccgtatt ctttcagctt gcccagcgta ccatcagcct
    17761 tgttgctttt gtaggtggtt atcaacgcgt tgccgatcag tgcccctata tcgccctctg
    17821 tggcttccgg aagcttctcc tgcagcatct ctctggctcc tgaaattttc cggtcgtatt
    17881 cccgtttgag tttgacataa ttcacttttt catcagaaaa agtgagttct ttccccaaaa
    17941 tacaaaatgt attttccata tgcatcctcc ttgaaaaaat cctaagacaa cgccgttcca
    18001 gagaaagtac gtcgagcaga ttacgtgcaa agccgtcagg caggctttat gcagtgtttc
    18061 ctttatgcaa catttctcta agtttgtgtg gcattatctt tattataaac cacataattt
    18121 ctattgtcaa gttatttttc aacttaccat taacaaatga acttgctttc gaaatttttc
    18181 gatcgttttc tgtggtgctg tttttaggaa acattggata aagcttgtgc gtaagatgcg
    18241 tcgtcagatt tttcggcaga ttgcgtgcaa agccgtcagg cgggctttat gcagtgtttc
    18301 cttttttatt atacgcggag aaccggacat acctgtgtcc gttcaaagat aatttacagg
    18361 agcacatgca aaacatacag ctgggaatgt ctgtaccgca gaatcgcccg atagacttgt
    18421 gtgcaatctt tctgtgttgc ccttgatttt atcccagtaa tatggtagaa taaatacgac
    18481 agaaatcata cagttgcgat gatatttata agtggctcag agcagctgct gcggcagact
    18541 gggagcagga ggaaaagcag ctgcaagcgg gtgcacttct ttataagaaa agctggcatt
    18601 cgcgttacgc gatcaagggc aataccgcac aaagcccgcc tgacggcttt gcacgcaatc
    18661 tgcgtgcaga ttttccgtca tattgcccag aaatcccttg ccatacggca gtatgcctgc
    18721 ggaatttcta ggcaatctgc cgaaaaatct gacaacgcac cttacgcaca agctttgcac
    18781 ggtattgcct atagaaacag agatcaaaac cacgcttggg gaattgggta tcacatatcg
    18841 aggagtttta cacatgacaa attcacacca tggggtacca gaatcaaaaa ggagcgtgcg
    18901 tatcatgcaa aagaaaatat ttgcctgggc aatgagcatg gcggtagcgg caatgattac
    18961 ctgcaccggc tgtgcacaga acaacaactc gtccggcaca gacagcggca gcagcactgc
    19021 acagactacc acgctggacg ataacgacac agcagaagct gctgtcggac agagcctttc
    19081 ggcgatacag tccggcgaag ccggcgtgac cacagatgaa gatgacctct gcgacaacta
    19141 tgacgcattt gatgcagaga tcacgctgga ggacgacagc accaaggtga aaggcaatac
    19201 caaggcggtt tccgtcaagg acaactgcat caccatttcc gccggcggca cctaccggct
    19261 cagcggcaag ctgaccaagg gacaagtcct tgtgaccggc tcggaaaaag tcaagctgta
    19321 tctggacggt gtggagatca ccagtccctc tggaccggct ctggtctgca ccaacgaaaa
    19381 acgcaccatt ctgtcactgg cgaaaggttc acagaacact ttgacagaca gtgcggacaa
    19441 cgccgataca gagatcaacg gctgcaacgt ttccgcctgt gccctgtttg cacaggataa
    19501 actgacgatc aacggcagcg gcagcctgac tgtcacaggc agcagcgtgg acggtatcgt
    19561 ctgcaaggac gacctgaagc tggtgaatgg tacgatcact gtagaagctg cacaggacgg
    19621 cgtaaagggc aaggactgcg tggcaatgtt cggggcagac ctgaacgtca ccgccggcaa
    19681 tgacggcatc aagtccacag aatccaacga cgacaccaag ggctttctcc agctggagca
    19741 gggcagtgca acagtacagg ctggcggcga ctgtttgcag gcggaaacgc tggtgtggat
    19801 cgcagacggt acgtatcatc tgaccagcag cggtacggct gtgaatacgg aaaccggcga
    19861 agccaacagc agcaagggaa tccactgcgg cggtgatgtg gaaattgccg gcggcaagct
    19921 gaccattgat gccgcagaag acggcgtgaa ctgtaccggt tcactggagt tacaggcggg
    19981 agaagtcaat gtgaccagtg ccgaggacgg cattcaggca gacggtgacc tgacagtttc
    20041 cggcggtgct gtgaccatta ccaccaccgg tacagttgcc gcttctgcac aggacgattt
    20101 ccagccgaac aacttcggcg gcggcaatcc gccttccggt gacatgccct ccggaaaccc
    20161 gccggagctg ccggacggcg aaacctttgg cggcggcaat ttcagcggca acggcaatac
    20221 accgcccgac aatgcagaca accgccagaa cgccgcagcc agtgatgcca gtgcgattcc
    20281 ggcagcagcc atgcagacca tcgcagattc ctccacagac gcaggagcat ctgcggcagc
    20341 tgccatgacc actgctgcgg aagatgccac cagcaagggc atcaagtgcg gcggaaacct
    20401 gaccatgagc ggcggcagct gcaccattga ttccaccgac cacgctgtac atgcggtagg
    20461 aacggcagtg ttttccggca ccacgctgga gatcacctcc gacaacaagg gcatcagcag
    20521 ccacggcgat ctggaaattt ccggcggaga tattacgatt cacagctgta ccgagggtgt
    20581 ggaatccaag gcggaaatga ccatcagcgg cggcaatgtc cggattctgg acgcatcaga
    20641 tgacgggctg aacaccggcg gctctgacag cggctcttat gccatgacca ttaccggcgg
    20701 ttatatctac gtcaacgctt ccggtgacgg tgtagactcc aacagcacat gggagatgtc
    20761 cggcggcact ctgctggtat gcggccccac cagcggcggt gacggctcgc tggacgcaaa
    20821 cggcaacatg acctataccg gcggcaccct gctggcactg tccagcaagg gcatgatgga
    20881 gtatccggaa agcggctgtc tgatcgccac caactgcaat gccgcagcag gggaacagat
    20941 cagcattgtg gacaagaacg gtacggttct tgcagcattg caatccccga aggccgtttc
    21001 cgatgtgatc tatggcattg gcgacagcga ttccgccgac tataccattg ttaccggcgg
    21061 cacctatgac ggcaccctca acgaggacgg ctatggagaa ggcggctctg tcagcggcgg
    21121 cacagaggtc acagcaaacg gcggcaccgg cagcatgaac ggcggagcac ctgccggcgg
    21181 cggacggggc ggtgctccca atggtgcacc aaacggcgga cagggcggca caccgccgga
    21241 aatgccgaat tcgaacaact ccggctctta aattagagtt tcacaagtga tcttcagtag
    21301 tcttctttgc agtgcacaca ggcggaatgt ctgtgtgcat tttgcggttg acggcaaaaa
    21361 tttcaggtgc tgctctgcac aaaccgtcat tttgtgcaaa aaaagcacga aacagaagca
    21421 gaaaacattg cactatatgg aaaatacttg acaaagtggt gtaccctctg ttataataaa
    21481 agtgtgtgct gcggtgcctg cttcgcgcag tgccgagatg cagttttgcc ggaattcttc
    21541 cggcggatcc tgtggaaagg aaagcatgtc agcacagaaa tgtgctgtga gaatatgatg
    21601 aaaaaaaact ataaccgaga tcagttcaaa cggatctttt cattttttgc agcggtgctg
    21661 ctggtggggt tgtatgccgc cggctttgca tgggtgtggt atgaaagcta cagcgatgcc
    21721 attctgctgc cgttctatcg ccgcggcaac tgggtcatga tcctgatcta tgtggtgctg
    21781 gttctgctgt ttgccaagtc atttggcggg ctgaagatcg gctacctcaa gcgaacggac
    21841 atgttctact cccagacgct gtcgatcttc tgtgtcaatg tggtgaccta tttccagatc
    21901 agcgttgtca accgtgattt ttctgcggca gtgccgatga tcctattgac cattgccgat
    21961 ctggcggtgc tgattttgtg gatcgtgctg accaaccgct tatgtttccg gctgtatccg
    22021 ccgcgaaagc tggtagtcat ctacggcgac cggaatgccg cggagctggt gctgaaaatg
    22081 agccgccgcg tggacaagta tatgatctgt gaatccgtca gtgccaacgc accgccggag
    22141 gagattcagg aggcaattgc acggtacgag ggtattatcc tctgtgaggt ttccggaaag
    22201 ctgcggaacg atcttatcaa atattgcttc gcccagaatg tgcgggcgta tattgccccg
    22261 aaaatttcgg atattatcct gagaggggca gaggagatcc ggctgtttga tacgccgctg
    22321 ctgctgtgcc ggaactatgg gctgacattt gaacagcggc tgggaaagcg tatcttcgac
    22381 cttgtttttg cggtggtctt tctggtgctg ctgtccccgc tgatgctgct gtccgccatt
    22441 gccattaagc tggacgacgg cggtccggtg ctgttcaaac aaaagcggct gacctatcac
    22501 ggcaaggaat tttacgtgta caaattccgc agcatggtgg tcaacgctga aaaggccggc
    22561 ccgcagctgg caaaggaaaa cgacagccgt atcacacggg tgggacggat tctgcggaag
    22621 tgccgtctgg acgaactgcc ccagctgatc aatatcctcc gcggcgatat gtctgtggtg
    22681 ggcccccgtc cggagcgtcc ggaactggcg gaggaatacg agaaaaccat gccggaattc
    22741 gagttccgac ttcaggtaaa ggcaggactg actggttatg cacaggtcac cggtctgtat
    22801 gataccctgc cctatgataa attgaaaatg gatctcatgt atattgagaa ctattccatt
    22861 tttctggacc tgcgtatcat tctgatgaca ctgaaaactg ccctgttccc cggtgaaacc
    22921 aacgaggaag ccaatgctca cgatgcacag gaactgctgt atcaggagga gcagatgcag
    22981 gagcaggaac aagagaaagg agatcactca tgaaagtgac cgcagtaatc atggcaggcg
    23041 gcagaggcga acgtttctgg cccaagagcc gtggcaatta tcccaagcag tttctctctc
    23101 tgaccaaaga cggagaaacc atgatccaga aaaccgtcaa gcgtctgctg ccgatggtgg
    23161 caatggacga cgtatttgtg gtgaccaatg tcagctatgc cgcactggta aaggaacagc
    23221 tgccccagct gccggaggaa aatatcctgc tggagccctg tgccagaaat acagctccct
    23281 gtatcggtct ggctgccgct gtgattcgca agaagtacgg cgaggcaatg atgctggtgc
    23341 tgccctctga ccacctgatc cgttatgagg aaatgtatgt ggacaccctg cggcaggcag
    23401 tggcagtggc agaaaaggat cagaatctgg tgaccatcgg cattacaccg acctatccgg
    23461 aaaccggcta cggctacatc aatttcgaac gggacaccga tctgggtatg gccggcgttt
    23521 atcgggtaga gcgtttcgta gaaaagccgg atctggaaac tgcaaagcag tatctggctt
    23581 ctcaccggta cctctggaac agcggcatgt tcgtctggaa agcatcgtcc attctggcaa
    23641 atctggaatc cctgatgccg gacatctacg ctggtctgga acgcattgaa gcggctgtgg
    23701 gaactgcgga attcccgtca gtgctggaaa ctgagtatca cgcattccgc agcgagtcca
    23761 ttgacttcgg tgtcatggaa aaggcagaga acatctacac tctgccgggc agcttcggct
    23821 gggacgatgt gggaaactgg ctggcagtgg aacgcatcaa ctccaccaat gaatacggca
    23881 actatatcga gggcgatgtc atcacgatcg gcaccgaacg cagcaccatt tgcggcggca
    23941 agcgtctgat cgcagcagtg ggcgtggaag atctgatcgt ggtagacact gacgatgccg
    24001 ttctgatctg tgccaaggac agcacacagg acgtaaagaa ggtcatcgaa aatctgaaga
    24061 tctgcaaccg gaaccagttg gtataaggca acaccgcaca aagagcgtct ggcgactttg
    24121 cacgccatct gcttgcaaat
//
LOCUS       DAWBGR010000012        24085 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_774_length_24085_cov_3.869080, whole genome shotgun sequence.
ACCESSION   DAWBGR010000012 DAWBGR010000000
VERSION     DAWBGR010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 24085)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 24085)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24085
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_774_length_24085_cov_3.869080"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4592..4601
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6104..6113
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5062 a         6953 c         5620 g         6430 t
ORIGIN      
        1 ggaaaaaatt ttcgtttcgc tatctggttt tgcattcatt ttgaatatga aatctctaaa
       61 cttgtctaac gtcttttcgt cttctgacac tttcaattcg tttttgcatt ctaattgtga
      121 tgccaaaaaa atcccccctt ttttgatata tcctaagttg ataagaaaag catctatata
      181 tttattttac catattattt gtaaaagtca agcgaaaaac gaaaataaaa atcagaaaag
      241 tccgccgctc catacttctg gagcagcgga ctttttgcgg atttatccct atttacactt
      301 cccgaaaatt atcccaagac acataccccg tgacatactt ccccgccggc tttttaccgc
      361 aggaagccgc cgtagtggtg atgcggtacc gtcccagctt acaaggtacg ccgtcgtaga
      421 tgtagtacgt tccggcggtc agtcgggcag ccggcgtgga ggtagtttca tttgcaaaca
      481 gcggcacgtt gctgccgatg tgcaccgcct gacctttcgc aaacttcccg gatctgctgt
      541 tggtatacac cgccttccca ctggaatcga aaacggaata ccctgccttg cacgccttct
      601 tggcgttctc cagagaagca tatgcgccga gctgactctt catatcgttc gcgctctttc
      661 ggactctgta cagctgcgcg ttggatggct ttgcggcaga agtaccattc agataggctt
      721 gtacttttgc tttgaacgcc gcccaatgcg gcagaatgta cgccggacac attttatagc
      781 tgttgtgcat ggtgttcagc tgatccactg taccacgctt gccgtcacgg acattcagcc
      841 aatgcgtgtg ggtgtaaagg tggttgatgt caagtccgta cttcttcaga agtgctgctg
      901 caagtcttgc acagttgtcc tcggatttct tatctctgtc gttatacgca gatgacataa
      961 tacattcgat tgcaatggtt cttctgttgc cattgccgct accgtcagca gcgtgccatc
     1021 cacttaagga taaaggcaga ttctgccatg cacacacatt atccacatag taatgaactc
     1081 gcacgtcctt catattgcca ttaacggttg ctcttgtata ctgttccgca ggtgtcgtgc
     1141 ctcttgctac ggtgatccag tctgtgttgt gaacggtcac cccgatgatt tttcctgcca
     1201 tgggaacaga tggcatgtca atacgattag ggttgtgctt tgtcagcaga tactccttga
     1261 cggtcacacc gccgagcgtg gttgttttgt ctggggttaa aattgccata attaatcttc
     1321 ctttcccttg ctttgcagca agtcaattgc tttttttaac gccgccggca tggggattcc
     1381 cattagtccg gcgttttcaa aaatggaaat caactcgttg acagtaaacg cgatgcagac
     1441 tgcatcacgg atatacgttg tgccaagaat cagatccagt tgtgcagaga tcagaaccag
     1501 cagcagcgtt acacattttc gggaaacgcc tttccagcca atttttgatt gcagaccgcc
     1561 ggaagctgtc ttgggggatt tccggaatac gcctgccaaa atcaaaccgg tcaaaaaatc
     1621 gattgccata aacaacagca acgcccgaat agatgcgtcc caaccaccga acagcccggc
     1681 aataaagctg ccgacaatac cggcggtggt gcagatggtt tctttcatag tttcctccta
     1741 gtcgagttaa atgttaatct acaaaaaacg tacaatgcat gtaatagtca actcccgtag
     1801 taaaaattat ctttttctac ccaagcagat atgttaaatc aacgcgatac gtagcttagg
     1861 ttactggata cgaaaacatc agtctgtaaa tggtagatgc cttaaaatta cctcttaaat
     1921 ataatttacg gtctggattg taagatatgg ttgctataaa gctttcagtc ccatcattct
     1981 tactgcccac tccatatacg ctcgtagccg gagtaacagc aacaaacgga tcaattttag
     2041 tcacgtcaca aatcgtaata ctggtcatgg caacgttcgg tgtaaaactg catttaaccc
     2101 atgcaatctg accgaccctt cggaattccg tgcttatatt attaaacttg cttttatcag
     2161 cgatataagt agtaacactc gaccacccgc tatcagtaag agtagtggct tcggtggctt
     2221 tagctacagt gctggtcaga aatgcctggt catagtatgc tgtgtcagca cctgtgcctt
     2281 ctggttcctc agactgtatc aaatgctgag atgtccaaga tttgtatttg ccatatacag
     2341 cgtaatttcc gttccagtaa ccccaaggca tatacgccca aacggtatat gtgtcatgtg
     2401 ctgtaggtat cactttgact ttgacactac tacagccgac gcgatagacc gtaacgccac
     2461 acgcttttgt cgccgattct gtggattgcc atccgtcctt gatctgaact tcaaaagacg
     2521 agttctggtt cgcacgacca tttgctccgt cgccgctcca cacgcgtatt atggcattgc
     2581 tgaaattacc ggaggatacc agcgtgccaa gcttaaccca ttttgcagtg ttgttggcac
     2641 cgtttaccca aagagcactg tttgttatca tcgcatgtgt atgaccagat gccgacttgc
     2701 tgtccagtgc ggcttttacg gctttgttct gcaccggatt tgtggaggta gcagacaagg
     2761 cactgtctac cgcgaccttg gttgcaccag cagcgatacc gtccagtttc ttcttatccg
     2821 ctgcactcat cagaccgtgt gcagactgtg tggcatcgct gtaggtggtg tcagtaaatt
     2881 tcgcatttgc cgggacgtca gcgttaatag agtgtgtaat ggcggtagcc gcaccattcg
     2941 cagcgacata tacaggctta gtcgctgaac ctaccgcggt attcgctgtg tgagttactg
     3001 ccgtggattt gtcttgcttc ccatcaagct tttcatcatc cggaattccc aacgccatga
     3061 tatctgcttt cgtcacattt accgcgtccg tgacatgccc ttcgctgttg ttagcgaatt
     3121 tgtaaaatcc ttttgcgtga gaagtgtggg acgggtgggt gtaaattgta tcatcatccg
     3181 ttacattgta aaccgttccg tctgatccag tcagtgtgat ggtgtgcccg gatttcgata
     3241 atgcgtattt tgtattcaca ttttcgccgc taccattttt tgattttctg tataggtatt
     3301 tgctgtagtc atttgccttt ttcgccgcgt ttctgtttgt gcttacagat agcgtgcggg
     3361 tatcttctcc ggcgaatgaa atttgctggc cgccgcggaa tgtccatgta acgtctgtta
     3421 catatgattt gaatttttta cctgtgaacg ggtcgataat cgttaccata tccccaacac
     3481 gaaaataata ctggacaaat acggtgcagc gaaacggatt gaaacacggg tttccatcgt
     3541 ccgcgtatgc cgcgtcgcaa atatacgacc acatgtattt tatcatttgc gttactttgc
     3601 caccgtactg gttaggaatc atatcttcct gtgcctgcca gaacgggttg ttcgtcagat
     3661 tgacagatag cggtgcagcg ttatctggtg tatccgttgt tctacgccac cagccgccga
     3721 aaccgctgtc aaaaccgatt ttccatatag tgggtgctgc atagtacggc gaccgctgat
     3781 agctaccacg ttgtatgttg gtttcattta gttcaacgcc gcatttgcga tccccaaaac
     3841 ggcagaaacg cagccggcca tccggagcag gcacgataaa acagcccagg tattccgcca
     3901 tgtagttggc aaaatctttg acagattcaa tgctgcattt ttcagaaacg catgtgcaca
     3961 acatagtgtt tccggattcc ggtatgattt tctcattttc actggcatta tagtgcggaa
     4021 cgccatatgc gttgcaaatt tggttaaaaa tcacgctgac cggtttaaat tttccggaca
     4081 gaacagtgaa cagccagttt tgtgcgtttt cggtgtcaac cgcagagtag cagccatcat
     4141 ccaacagccc cacattatca gagccagaaa cggtataaat accatagtta ttttcagcag
     4201 acgttacatt atagacgcca acctttgttt tgctgttgtt ttcataccat acatacaaat
     4261 gcacttttgc ttttgccagt ttggaatacg tcaaaccagc tggcgggcgg aatgacacgg
     4321 aaatcgtacc agcattgcaa cttccggctg ggatgccgcc actggaagaa cacgaagagg
     4381 atgtagtcag gctatcccgg ataattccgg attcatctac actgtatgtt ttcccatctt
     4441 tcgtttcgat gtcaatagcc gcatattctt tcaaaaccat ttaactgtat ctcctaacac
     4501 tgaaccgcaa ttcaaaccgc ttgaatgcgt tcatagggct ttcaatcaca atttttatat
     4561 ccgaatcgcc gaccgggatg tcgtaccatg tnnnnnnnnn ncatgtgctt gctggaatct
     4621 ccgtttctag cctccacgcc ttcaacgtat agtccgtttc gtcatttgat cggatataaa
     4681 aattcgggga cggcgtataa ccgtatttgt caatcatttc accgcacaaa taaatgccgc
     4741 cggcgtcctc gctagcctgc atgtatgtca ataacctatc gcatacatac acgccgtttc
     4801 cctcatagcg gcattcagac agtgcaataa agtgcgtcgg gtcagcgtct gccattccag
     4861 atatattccc gaaatatacg cccggcacat cgtttaattc aatccgcatc gccggtacat
     4921 ctatagtttc gcgcacaatt accggaatgg acacagaaac aatatcagca atatttgccg
     4981 gcgaaattac tgtgatattc ggatcaccgt ctgtgtagca taaaatgccc ttccatgttc
     5041 cgatgttcac gccgtctacc atcgcattcc ctatacagat ttcaccgttt gccagcactt
     5101 cagaaatgct cttgatttgg tcgcaccgac actcaaactg cagtgttgtg ctgatttcaa
     5161 cctttccgcg cgtataggtt gcatatgatc cgttttcaca tttttcacgt tctacgtccg
     5221 gagcataatc cgtagacagt cccccgatct gtgccaggtc gcacgctgca taataaatcc
     5281 tatccatgta cggcgttggc gggttgttat aataaatgtc gcacggctgc aattctctca
     5341 tactcctttt tttaaaattc gtttccgccg ctgatggcgt tcgcacggtc ttttgcacgc
     5401 agcacaattg tgtctagtgc ttgtgtattt ggtgtcatgt agatcgggaa aatccaatct
     5461 tttcccgcac cgttctgctg caactgccgg tattcctcct ccgctgtttt cttgggctgt
     5521 gtatactgta tcgccggaac agcggttccc aatccggaat agccctgcat ctgtaccgca
     5581 gaatccagct gcatttgcag tctgtcggtg tccaccttgt ccatcatggc atccagcgaa
     5641 cggttcagat catcggcagt gtcgcctgtg gtgtcttcca tgccgactgc aacgcctggc
     5701 aaaaggaatt taccgatggt atcacgcatg agcttagacg gggaattgat cccgaaaaag
     5761 tccttgaatc cgtcccagat ctgtccggca acgtcctgca ccgtatccca gatagcagaa
     5821 acaccctcaa cgagaccatc tgcaatgcct cgaagaatgt tttttcctag ttccagccag
     5881 tccacttctg tgattgcgtt ccagatgctg tcaataattt ccggaatttt ttccaccaga
     5941 tccggaatgt gcttgatgag gcctgttccc agtttcagca taatctgtag tgcagcatca
     6001 atcagctttt ccagattgtc cccatccaga agagaatcca ccagcttttc aatgatctgg
     6061 attgctgcat caatcagttc gtccagatta tcaagcaatc cgtnnnnnnn nnnccgtcca
     6121 caatggcgaa aatcatatca atggcggcgt tcagaagttc atccagattg tccagaatgc
     6181 ctgtaacaat ggcaaggaga atctgcactg ccgcatccaa cagcagcggc agattgtcaa
     6241 tcaccagagt gacaagggtg tccagaatag aaatcactgc atcaataatc attggcagat
     6301 tttccagaat gccgttgata attgccatga gaatttcaat ggcagccgtc agcaaaatgg
     6361 gcaaattttc tgtaatcact gtcacgatag ctgtcacaat ctgcggcaca tactccatca
     6421 gcagaatcag tgcatcggat aatccgtcaa tcaaagacgt gataatctgt atggcaatgg
     6481 gcagcagctg cggcagtgcc tgcacaatgg tttcggcaat ggtggacaaa atgccagaag
     6541 ccgtttccat caaggacggg agattctctc cgatgctgct gacgagttcg tccagaatgc
     6601 tttttccggc ttttgccaga ccgcttgtgt tctgaccgac cgaatccagc accgtctgaa
     6661 cgctgttcat gagcccgtca aagattcctt ctgcatctgc gccgttcatg acgttgatca
     6721 caccggaaat ggcatcggca aatccgccgg aaagtgtgct tttgatgtca atggtgctgc
     6781 ttgaaatctt gcgtttcagc ccctctacag cactttccac atcgtcatac ttgacttcat
     6841 tgatttgccc cagtgcatca tacgccacac cggatgcatc ttccatgttt gccagcatgg
     6901 gcaacaggtt tgcctgtaag tcctcgaatt gtgtcccgaa caggtcaatg gcagcttggt
     6961 ttttcgccac cggatcggca atgctgtcca acgcctgcac cgtctggaaa aacgcctcct
     7021 gtgctgcgtc accgccggca gcaaaccgct gtgccatgtc atctgcattc atgccgatca
     7081 tggcaaaacc ctctgccgtg gaatcactgc cgtccttgca gcggatattg aactccttga
     7141 ccgcatcgcc caccttgtcg atggagaatg ctccggattc tgcaccggag atcaggctct
     7201 gggtgaactg ctctgcggaa agtcccaacg ccgcatactg cgtagaatac tcgttgaggg
     7261 tgtccagcaa atcgccgttc tggtctgcac cattctgtgc tccaacagca atgaggttat
     7321 atgcttcttc cgcagagatg ccgaagttgt tcatcagtgc cgaagccgcc cgtgtggatt
     7381 cgtttacctc atagccgaag gtgtcaccca gtgcaaaagc cccctctgtg gctttctgga
     7441 gttcctctcc catgagccca gtctgtttgg agacctctgc cacggcgttg gctgcatcgt
     7501 cataggattc gccgaagttg ttgccgtaga catccttgac cacatcacgc agtccctcca
     7561 gctctgcacc ggttgcaccg gtagaagccg ccatctggtt gattgcctga ttgaactgat
     7621 cgccggacga gatcatatcg ccaaatgcag acagggcttt ctttcccatg tcagagagca
     7681 gattgcccat cgccacagat gccgcagaga ttgtcccttt catgccttca accttttggt
     7741 caaagccgct ggtatccccg tcaataggga ctctgatgct ttcgtctgcc atagaaacca
     7801 cctcctgcag ggcagaaaaa tagcgttcgg ttgtcccgaa cgctgctgtt ttgtgtatga
     7861 aaatgcaccc gatttctcag atgctgattt tacaaataag aacgccgtac ccacaggctt
     7921 ttcctgttct tgttttccgc cctccgccag tttatgcccg tggtcggggc ggtaattaca
     7981 gttcgatgtc ttcaatcgct gcacgtgctt ccagactggc gatataatct gccatcgctc
     8041 tgatctggaa attatagaca cctctcggac aggacggtgt gaagcccagc tccccagtat
     8101 cccatttgtc gagcatcgct ttcagcttct ggtaccggat gcacacttgc atatactccg
     8161 ccttgaatcg ctctttgtaa tcgatactgt ccatcaagct tacagtatct ctcagttctg
     8221 catgtctgta tgttgccatt attttctcct ttcgggcatg aaaaaagcgc accgtgcggt
     8281 acgctttcca tatttttctg cattgttttt taccattctg tgataacaat ttcactggca
     8341 acttcattaa gcgttttacc ttttatgaat ggtgttgaca tcacatcatc tatactatgc
     8401 acagtttgtt cctcgccgtc aaaataaagc aagtattctg tggattttgt ttctggtaaa
     8461 taatatgggt ctacattacc atctttacca cagtattcaa aaccaaaaag tgtggcacat
     8521 tctccaatgc gagattttat ttgctttgcc gtcataatat atcaccattt tccaatcgtt
     8581 cactatcgtt gagttctctc gcttttccat gaactacatc gccgttttca ttaataaaat
     8641 aatcgtgtgc gtgttctccg tgctttccga atttgcgttc ttctttatgc ccgtggtcat
     8701 tgtttgaaat ttgttttatc tgaatcccat ttgaatcgta gtaatttcta tcaattccgc
     8761 cttttgcgtt ttctctttgt gtaatgccat ttgacggacc atttcttact acaatttttt
     8821 tgacaacaaa aaccctgttt ccagccgcat cctttgtttc ttttattata ccaccactat
     8881 catccgatgt caacgccttt ccagaaactt tctcagcttg atacaacaca ccgctgccgc
     8941 cgttcgtctc cggtgttgga acatccactt ctacagcctt gccgcttgaa accgcattct
     9001 ccgcagccgc ttttttcgcc ccatgcaccg ccttttgtgc ctcactccgc ccgaagcctt
     9061 gcacttgtgt ccggaacgta tccttccgcc gcttcgtctg cttgcagaag tcgtccagct
     9121 cccgttcaaa gcctttcagc ttcgccgcag ttgccgtgta ttcgctttcc agtactgccc
     9181 ttgtctcatc atctgatgca gcttttaccg cttcctgtgc cgccagaaca cgccgcttct
     9241 gtgctctgat cttgcgttct ccggcacgct gcatctggct gacctcatac tcggtatatt
     9301 tcttgccgtt gtaagaaatg ctcttttcgt ccagtttggc gatctcctcc ggtgtgtagt
     9361 tcggcgtaga cagccccgga taatacgggt gccagttgtg ccggcagttc caacccttga
     9421 agcctgcacc gtctccgtag ccgatttctt tcagcgtgaa tactttcaga ccgtcaatgg
     9481 ttttttctgt gcgttctcca cgaatctgga caagctgccc ctgccatctg gcgtgttctg
     9541 gtcttgcacc gccgtgtgcc gtcagttcca tgtactgaca gcccgaatcc tccgcccgtt
     9601 tcttcgccac cgccgccgat gtttgcccaa cgcctgtcaa cacacaccgc cgcaccgcca
     9661 cgtcaattct gtccttgtgc ccggtgggat aggtgatgtg tgccccggta tccgccagat
     9721 tccgcaccgc catacggatt gcctgctgat agctgaacgc accgcttgac acctgcatat
     9781 aggctctgtc gcagacctgc aagaaccgga actgcgtggt gctggctgtc gtactcacca
     9841 gattccgcat ggtgcccagt gtcttgcggt atccggcttc cagcacctgt ttcatgccgg
     9901 cgtcctgtcg gatgtccaca ggcacttcgc ctgcctcctc gtgggcttcg ttgtcgattt
     9961 ccaccgtctg cacaccggca tcctcaaaca gtgccttgac ctgtgcaacg cttgcatccg
    10021 accgctgtac aatcatccgg acgatgtcct catacagcaa accggaagct tgcagcatct
    10081 ctgcctgata cgccgtgctt tctgacacaa agcccatttt cagcatacgc cgcaccatgt
    10141 ctgcaatgat atcatcttcc agctgctgat acagtgccag aatctgcgtc acgtccggct
    10201 catagtcacg catcacgcat caccgccgaa caacccgccg tcaccgttct ctgtcggcag
    10261 atattccgcc gctttctttt catcgcagcc gaagtaccac atcacaaact gctccttgcg
    10321 gtacagccct gcactgacca tctgcagccg ccgctggaat tccttgtctg catcctccag
    10381 cacaccgtct ccgaaggtac aggacagctt tgccggtgtc tgtgtgcggt tggcataata
    10441 gtcccgataa tactgcatcc catagaccag atcatccagt gccgtacgca aattcgactg
    10501 caagtcgctc acacgcacaa agctgcgctg tttggaagta cgaacttctt cggcagtctt
    10561 atctacatcg gacacctcgg acagcgtacc ataggcaagc cccacggcat tctcgattcg
    10621 ccgcagaatc tggttcaatg cgttgaaata ggacgtgtca cgaacttccg gcgaaaaggt
    10681 attgatgaat gtctttccat cggttttctc gtagctgcgg aacattcgct ctctgccttt
    10741 gggcaatacc ggcttgccgt ccttgtaccg gaacaagtcc tcgcttgcat caatggcacg
    10801 ttctgaggat tccaattccc agagaatgcg ttcccagtgc acatctgcat cacgcagaaa
    10861 cccgactgca tccccataga cagaaacgcc aagaggagaa gccgggtcga tgttgttgga
    10921 atcgggcgtc tgaaatacgg caaacagagg acgctgaaca ttttcataag ttttttccgg
    10981 cagaagatct gcccatgcag gtacttctgt aaggctgcat tccgtgccca gcgtgccggg
    11041 cattccagac cgaaagcatc gctgcgaaat ggtgtgagtt tgctttgccc ggtcatagac
    11101 gtggtattcc aatcgggtat agtacgtttt ccccactgcc agttcttccg gacagattac
    11161 cgcatcgcag gcggcgtctg tataattcac cggcaaatag gcgttttgcg gcaccatgtc
    11221 caccagcaca ccgccggaaa aatacggttt cagcagcata ccgcccatgg caagcccaaa
    11281 atccaagctt cgccggagca ccggtattgt cttttgcaat atcccatcca gttccgtatc
    11341 ctgcacagaa gcggaaaact ccgtcatcgt cagacgtttc agctcccgta caattgccgc
    11401 aggcagccgc ataggtttca ctcgcttttt ctgccatccc gtcttattct gatacagatt
    11461 ttcccacagt tccatttgct gctgcatatc gctgctgacc atacacggca cctgcatcgt
    11521 tgctgcaatg gtattggcgt caatcatgct gcattcctcc ctcctgtctg atttcccgca
    11581 atgtacgaac cataaccgtc cgaacaaaat agcgtatatc gtccatagcg tgatcgtttt
    11641 ccttgatgac ggtgtccgct tcggttttct catcccaaca ataaagacca aactcccgaa
    11701 tgcagtctgc acagttctcg tgaaactgca aatatccaag ttgtaacaaa gtggaagtat
    11761 cccgaatgcc gtccaacacg ctgttgtctg cctgccttac ccggaataga tgatgccgcc
    11821 gaatgcactc gatgaacgaa gcggcagacg gatccgccac tacatactgt atctgctgca
    11881 ccagatctcc agcaagctgt accaactctt tgtaatgctc ctcgtctgtg cggcgctgcc
    11941 cagtctgtcg gctgttccag taatactccc gaatacgcac ggctctcccg gtgtatcggt
    12001 ttactgccca tagtcccatg ctggtgggat tcactgtgcc gtaatcgcag gagatgaagc
    12061 aatcgcattt ttgcaagtcc ggcatttgct gtaccacatg cctttccttg tcaaacatgg
    12121 gatagacaag cccctctgcc acgcaccact cgccgagtac ataccgccgg tggaacacac
    12181 cagaaaacat agattctgct ttgcggatct tctccggcgt cataatcggg ttgtcctgca
    12241 taagaaagtg gatgtgtctg gtgtcgctgc gttcgccgcg gtcgcacttc tctacccact
    12301 gtttgtggaa ccagtgctcc ggcgaatcgg gattgcagtt aaaccatagc tttgcatctt
    12361 ctacggaaag cgttcttgca atggtttgat tcacaaaaga ttccggcatc agtgccactt
    12421 catcgaacaa cacaccggac aacgtcaatc cttgtaccaa tttgtaggac gcttcatcct
    12481 tgccgccgaa cacatgaaac cggttttctg tgccgttccc tgtaatggtg agaatgtgct
    12541 tgctccccag atagcggagt ttgaaatagt gggtgatatc caccatttgc tccagttcca
    12601 tgatgatgtt gcgctctgcg gattgtaccg tattgccaca gacggcaaat cttgcgtttt
    12661 tgaagcgtga cattgcccac aaaatgaagc tgcacgccat agaagcggtt tttccggagc
    12721 ggacagaacc gtcacagatc agtgcatagc tgtccggttg atagcaccac cgaaacacct
    12781 gcttttgttt ggctgacagc ttggaaaatg tcatgtgtca cctccgtctg agcataaaaa
    12841 atgcacccgt ttccagattt tttatccaaa aaacgcaccg cattgatagc cgtccagctc
    12901 cggctgtgga atacgtatcc gctcctgaat cttccggatt cgcagccgct catttttgtc
    12961 tttcaccgat gccagattca cactgcggta tcccatacgc tgcttaatgg gtgtttcgtc
    13021 cggaagggca tcaaacagcc ccagaaacag atgccagtgc atctgctcca cctgctgcaa
    13081 gtccatgtga tagaccgaca ggaacgccgc atacacatag gctgcatcat actgccatga
    13141 cagcacccga tctgtggctt tgcgttcgga atcgctctgc cgctgctgcg gctgctcctc
    13201 ggagcgtgtc gcaaacccga tgagagcctc caaagcctcc tccagacagg aagccggcgg
    13261 cttgtccatg taccattcca gcatcagcag cagcttgtcc cgtctggaaa tgctatcgtc
    13321 ctcctgcata tcgtagaacc gcagccagtc ccgatagtcg gtatagatcc ggtacgccct
    13381 gccgcccacc gtcacagtat ccggcagggt atcgtacagc agattcaacg cttgccgcct
    13441 ttgggcaggt acttcttttg cagctgtctt tgtgcttcct gtgcctgtgc cgcctgtttt
    13501 gccacaaagg tcagaaattc gccgtacact gcggtatact gccgtgcatt gtccgaaatc
    13561 ccggcaaaga tctgctccgc cgtaccaccg ccgaacaggg tgtcatacag cgtgcgaaac
    13621 gccttgcagt acgcacggat atatgccgca gtaccgccgg acttatccgc cggcacattc
    13681 tgctccagct gtgccagagc agcctcatac ttttccacgg tatccgcatc ttcaatatcc
    13741 agatgcagtt ccagcccgtg aatatgccag attgtcagat catctttcat cattcattcc
    13801 tcctcacgaa gcaaagcacc tgatcgcata ggtattagaa cccagtgcac cgtttgtaac
    13861 agtaacataa atatagtcgc cgtcctttac ggtaaatgcc acactagaat tgataatggg
    13921 caaagaattt ccgttgcagg aagctgtaat gctaaacgtg ttgctctccg ctgttgcgta
    13981 cacaaccaga gaaccaatct tattgaccga atactttgta acagacggct tgaaggtcga
    14041 tttcagcaga ttctcgttgc cataagaaac agacagcgtt ttcagtactg gattggtgtt
    14101 ggaaggaatc gcaatggtct gaaagtcatc ggtgctgtgc accgtgacat cttccatctc
    14161 accccgtgtc ttgaagttgc cggaataggt catgcagtct gtggtgtcgc cgtcagcgtc
    14221 cggaatgacg gcatagtcac gcatctttgc ctctgccgac cagatgccgc tgccgtccag
    14281 ctgtgccgtg gtcatatcca cgctgataat cgaacgcact gcgtcctgtc ccagcagctc
    14341 attttcatgg atattaatga tgtcatcggt caccggatgc ttcttgtatc tgtctagcgc
    14401 atatgcagtg gcaacattgt acccggtaat gtccgtccgc ttgaaatcct cgtccacata
    14461 ctgccgctca tattccgtcg gattcttcga tgtggaaaga cttgtaaagc cctccattct
    14521 tgtgtatgcc gagtttcccg gcaccagata aaatgccacc ttgccggtac gcagcacaag
    14581 gtctgcgttt ttcaaatttt tacccatgtg taaaatacct cctgtcctga taatacatca
    14641 gtcgtaattg tatctgatag cgagccgtat catcgccggt gtcataggca tagccgctgg
    14701 aaacaatgtc catgcggtat ggtgtccgat aatcgcccag ttccggaaag ataccctgcc
    14761 agttgttccg ctcgatccag tccgcaaagt cctcgtagaa accggagttt gcaagattct
    14821 ccagcacctt tgtgccgtat ttttcccggc tggcgaatac aaacaggaac tgccgcctgt
    14881 cgctgccgtc cgtgtacttc tgcaccactg gttcacatgg tgtcgtgtcc acagtatagc
    14941 caatggtgtc cgcttccagc tggtctacgc ctaagatcgc tccgtcatgg agcagcggac
    15001 agccggcaat gtactcccgt atcgccgaaa tgatcgccat tctatcaccc ctttgcaagg
    15061 atacgggcgt ttttcagaat aagcttgcca tggtctgccc atgcccgcaa cgcccacttt
    15121 cccccacgca acccacggga agcaccggca taccactgca cggctgcata aggtgttttc
    15181 cacatgagat agccgtccgc cggcttgctg tgggatatgc cggaatctcg gagcataccg
    15241 gtgtcaaacg gcacataggg atcgcatttc cgcagcagtt cattgcccac aatttcctgt
    15301 gcctgctgct tcagtgccgc cgattttgcg tgaagctgct tggtattgaa attgatcttt
    15361 actttgataa tcataccgcc gtcacctcaa tgtgctgcac accggcagag ccatagcgaa
    15421 agtctttcac ttccatgacc gtccggcatt cctccggcgg ttctgcatcg ctgcatctgc
    15481 cgcagaagat ccggtcgctc cgtttcggca gatagccgga aagagaagat gccggaatga
    15541 tgcacagcac gctgtcctgc tgcttcatgg acgtgccaga ctgcgtctga cctcttacat
    15601 cctcccagta gatcgtaggg aagaagtgcc gcacatacgc ctccatacgg tctttcccca
    15661 ccgttcgctc gtagacggta cagccgattt tgttggtata catcaatcgc accccctgta
    15721 caacagccca gtcgcaccca gatagcgaat gcagatggat ttcagataat cctgcaagcc
    15781 ggacgtgctg ccgtccagca gcacggagat gctttccgtg ggacttgcaa agctgacgct
    15841 gtatgcaccg atgctttcgg atgtcttcat gccgcttcca gatgctgcat ctcctgcacc
    15901 gtatgcctga taggtataca ccgcttcggc aagtgcacag cagcatttct tcacacggtc
    15961 ggtcaaatcc tctggaacac cgtccagcag ccgcccgaat gtcaccatgt ccagatactc
    16021 gcttgcccgt tctgccgccc gaccgaatgc ggtcgggtcc cgtatcataa cgcccaggta
    16081 aaaatcctgg tagtatggaa aatcggcgta tgccatgcct tacgcctcca ctctcttgac
    16141 gtagaccgtc tgcggcttgg aaatcccgat gccgtagacc tttctgccct gtaccgcaga
    16201 agatccgatg tagttgttgg tcagattctt gatttccacc ggcacagacc actcctggac
    16261 acggtgacac cagttcgggt gaccgcagat aaactcggtg gttgtcttct ttcccaccac
    16321 aagctttgta tcctcaaaca tggtgttgtt ggattcaaag acgttgtagc cggcaattct
    16381 gccaacaacc ccattctgta ccagttcctg cgacaaatcg ccctgcttga tgaatcggtc
    16441 gtctgtcatc aacacttcca tgaactccgg cgatgcaatc agccaacgct tgccgtcatt
    16501 gggcacgccc atacgggact gggtacgctt cgccgccaat accgccttgt atgccgtcgt
    16561 ttcagtgacg gcggtcttgg ttgccgatac agtgatgcct gcggtttcct ccaatgcacg
    16621 aatggacttg gtatccatag acagaccaag agaatagcct gcactgtcca gacgctctgc
    16681 ggtgatgccg tccggtaccg cctcggcatc ataaccgtca atcagttcgt tcaccgcctc
    16741 gtcaatgtca atgttcagat cgaagtaaga cgtagagctg ttggaaaggc ttgcaccgtt
    16801 ctgcttgtca tacttcttta cctcgacctc tgtatcacgc accgggactt ttaccttgcc
    16861 tgccttcggg ttgccctcga atctggaatt gaaaataagg ttgtcctttg tgaccagtgt
    16921 cgctctgagt ttctcgtcta ccatggatgc gtagcgttcc tgcaaaatgt gtgccataat
    16981 ctgatacctc cgttaagtta tttcttcaag tttggattca tttcataaaa tgcggattcc
    17041 acaccggaca ttgcttccgg tgggtggttt gacgtgggtg cagccgcacg ctcgctcgga
    17101 ttcggtgcaa aagcgtctgc atgagcttca cgaaattcat tcacatactt ttccgcaccg
    17161 accagttcgc cgccgtcaaa cttcagctct ttttccgtca gcaggtcggt gacatacttc
    17221 tcgtacacgt catttttcag ctgcaatccc ttgacatact gggacagttt ggtactgtac
    17281 tcgaatgcgg cacggtctgc ctcggactgc tccagcttct gcttgtagtc ctccacgctt
    17341 gccttgatgc cgtcaatgtc catgtcttta taggactgaa tggtcttgtt ggcttcgtcc
    17401 agctgcgtct gcacggcggc agcggcatcc tgttccgcct tgatgtccgc ggcataggct
    17461 tccgtgatct tctgcacggt gtccttgtcg gtgataccga tgccttccag aaatttctca
    17521 tcaatcattt gcttgtttcc tcctgttcct gcaatgcttc cagcagcttc ggcagttcgg
    17581 cttctgtatc gcctgctacg tcccgatcat ccggacggaa tccggcacgg tcgagaatgt
    17641 caaatgcagc tttcagcctg tccgcgtcct ttgcgtcgtt gtccatcatg atggcataga
    17701 cggtggcgac tgcgtccgaa gccgaaatgg caagctcctc ctgaatccgc cgaatcaacg
    17761 cagctttttg cttttgcaaa tattcctgaa cttcgggatt tttcagaagg tcacacgctt
    17821 gctgtgctgc cgatttcggg ctgtatccgg cattgattgc cgcctgctgc tgattcttgc
    17881 attttagctt gatgtattca tccacgaatt gcttacgctg ttctgtcagc atgctgtttc
    17941 accgtcctca cctccatttg tgcacaaaaa acgccgcccc aactctcgcc gggcggcgtc
    18001 ctctgtatat tccttgatgc tattattata ccacaggtgg tatgtatcat tctatatcat
    18061 ttttcagcat ttccaacgct gtaccatgcc acctgtgaaa tgtcatccac gagcacggca
    18121 tttccacgca cacttgttcg caggtcatgc cgtccagata ccgcagccgc atcagccgac
    18181 gcagttccgg cggcagggtg tcgatggcat tctccacggc tgcctgttcc tccagcagga
    18241 tgcactgctt tctccggtag agccgttcca gtgtttcctt cttctccaca taccgctgtg
    18301 cctctgacag cggttctccc cgctggtgcg gactgtcgcc gtatgtcacg ccgtgacagc
    18361 ccttgctttc ttccagttcg tgcagccgcc ttgtgatgtc ggcgagctcc cgccggatgc
    18421 tgccgtatcg gcgtagatcg tctttggtca ttgcatctcc tccagttccg gcagccctgc
    18481 cttctgccgc agttcattgc acacctgccg caggtcaatg ctgtgcagcg tcagtgccgc
    18541 atagtacggc gtaaacagtt cccgttccac cgccttcagc tgtgccagat cgttgtcgct
    18601 ccgggttctg gcatagcgtt ccagtgcagt gcggtaccgg ttcatctgcc ctcgcaggat
    18661 atgctctgcg atgcggacgc agccggtgtc gtccttgcag ctgtcagcac tgttcccgcc
    18721 gtccttctgg aacagcgggc agctgatcac acagtaggtt tcgtaaacgc tgccgctctg
    18781 tttctgctgg tgcttttccg cagtccagcc ctcgactggc acaaagcggc gtgaccagct
    18841 gcatccggtt gacgcactgg gcacggcgtg cctgcaccgc cagcagagcg tggctttctt
    18901 gatttgcttg ttttccatgt agatccactc cttttgggtt tcatactcgg tttacgctcg
    18961 gttaacgctt ggcgagtgtt agtcttacac tcggcgtgcg ttagcgtttc ttgcacttac
    19021 tcctcctcac acccgatgca ctcacgcaca atgccatacg cttcgtcgat ttcgatcatg
    19081 tcattgccga gaacatcatc gctggaactc caaaatttct tcctgatctg tttcagctgt
    19141 tccccggtga ccagcacgcc gggcttgcca cgcagcgtgt caaacgccgt catgatccgt
    19201 gatgccgttt tcttgtcctc gccgcagatg agatacacct cgtcctcaaa ctgccgtctt
    19261 gtttcttcgt cgatcattgt ctactccttt ccaactccac acaaccggtc atacagctcc
    19321 cgacacttcg ggcagccgtc tttccggtaa tagtcgccgg tcagctgcac ctcccgcaga
    19381 atctgcatat cctgttgtgt gatcttccac cgcagccgga acgcttcgta tccgctgacg
    19441 atctccgtct tttcccgttt cgcctgctgc cggctcaccg tccctgcccg aaactgggcg
    19501 tacagcgacc gcaggcagag gaacagatac cgttccggca cgtccagccc gtccggcaga
    19561 ggggcgttct ttgccgcaag gacttcaatt tctttctcag tcatcggctc tgccctctgt
    19621 cagataggtc accagcgtgt gtgcggcatc ttcccagctg cggcagacca cacagcagca
    19681 gccgttctcc cgcagccgtt ccagccacca catctgattg gcggacaccc tgcccttttc
    19741 cgttttcatc tccagataca gcccgtgata cacgcccctc gccacaggca gatgcaagtc
    19801 cggcacaccg gatttcaccc ccatgcgttt cagccttgca ccctgctggg gcgtacactt
    19861 tcgctcgttg ggaatgtggt acagcagtgc cagctgggga aacatcttcc ggacgctcgc
    19921 ctgctgagac cagcggatca ccgcctgctg tatctggtct tctgtcatat ttgccatttt
    19981 ctcacctcat catcagccgg ttcagaattt gcgatgcctg cagtttcgtc agcccgtcgc
    20041 agtccaccgg atacttcctg cacttcttcc ggatcagctc cacctgcttc tcgcttgccg
    20101 gagccgttcc ccaccgcttg gcacgggaca agtcccagag ataccgctgg tcgggataat
    20161 ccgtcagcaa ttcctgatac accttgtcga tcgcctcctg catgggcatt gccctgccgc
    20221 cgtacaccgt gttccccagt tcgtcctgac aggggatcac cagctttttc cggtctttca
    20281 gactgcacac cagcctgccg tccggcatgc ggaaaaagtg tatccctctg gtgtcgtact
    20341 gctgctgttt tgcccagagt tccacgatct gcgtgttccg gatccagctc tgtgggcagt
    20401 ccgaagccag ttccgccttt tccggcagtt cgaacagcaa gccctccact gcctgctgcc
    20461 ggctctgggg aatctgggac aggtctacgc ccagcagcgt gggtgcggtg cagagatgtg
    20521 ccttgcctgt cacgcccacg cagtccatga gcgtcagctt gtccttgtcg gggtgcagcc
    20581 gcagcccccg ccccaccatc tgggcataca ggctgtcgga ctgggtcggt ctggcgatga
    20641 tgaccgtttc caccagcggc atatccgttc cctcggtgaa caccatgcag ttcaccagac
    20701 agggaatttc ccgtgccgta aaccgccgga tgatctcgct ccggttgggc gtttttcctg
    20761 tgacggccac tgccccgtcg atccgcatgg cgatctgttc cgcatggcgg acgctggcgg
    20821 cgaagatcag cgttgcaccc ttggcgtatt tccggtacgc ctctgccacc gcatcttccg
    20881 tacctgccat tgcctcgtcc agttcccccg gtgcatagtc cccgttgcgg gtgtgtacct
    20941 gccgcaggtc atagccgatg tcgatgcggc ggcagaagat gtcacagaga tagccctgcg
    21001 aaatgcccca ccgcagatcc cgccggaaca cgatctcgtc aaacacgtcg ctgagccgtg
    21061 ccttgtcaga acggttcggg gttgccgtga acccgatgac tttgtgtggc gtgaagtagt
    21121 ccagcacttt ccggtaggta gatgctgcgg cgtggtgtgc ctcgtccaca ataatggtgt
    21181 gaaagcgttc cggctcgaac cgttccagcc ggtggacaag gctctgcacg ctggcagaaa
    21241 ccacctcgtc agagggtctg gaggattccc cagccatttc cacgccggtg cggcagccgg
    21301 tgtaataccg caggggctga cgcaccagtt cttcccgatg ggacagcagc agcacgtccc
    21361 cgtgccgcgg caggtgggta aaggtcgccg tcttgcccag accggtcgcc atctgcacca
    21421 gataccgccc gtccggcttg cgttccaaaa tgtcgataca ttcctgttga taaggtctta
    21481 actgcataag cattttcctt tctgttatag gctcccctcc cctaaaggag aggctatttt
    21541 ttgaaaattc ttgtgggacg tgtggaacac cgtgggacgg ctgtcccaca gaaaaagcca
    21601 cgcaatttcc ataatatatc cttgttgtgg gactgtggga caaattcgca cattttctat
    21661 ataggaaaaa tcatttcccg ttttcgtatc tttgcaaata taaactggtg tatatggggg
    21721 aaaaatgtcc cacagtccca cacgcccgaa actgcgtgac ttttggtatg tgacatgtcc
    21781 ctcaaactgt cccacattgc tgcaaaacag tcccacactc aaagcaattc ttcttctgaa
    21841 acgtctaaat cgctgttttc ctctgtgctg tcgtggtgca gtttcatcac aatacattcc
    21901 gtgttgatgc cgtgaatgcg ttttcctctt gtcatatttc gtcccctcgt caaaatcaat
    21961 cctttcgcct tgagccagct cagcagtgca cggctgtcat agccggcttc ctgtgctgcc
    22021 ctgcggaaca cgctgctgtt gatgtacgcc cagccggatt cgatcctgcc gaacacctca
    22081 ccgctgaggg tgggcagcgg tgtgctgtcc tgcatcatgc ggcgttccac ctcgaacttg
    22141 ttggcgttca ccgccaccca gtcgcacatg tactgatagc cacgctcgcc ggcggaaacg
    22201 ctggccttgg tctgcaaaaa cgctgcgatc tcgtccgctg tcagccactt ccccgtatgg
    22261 aacacgatct cgtccgccag cttgtccgcc gccagaatcg ccgcagccgc cattgcctgt
    22321 ttctccgtgg tgtcccgtgt ggacagttcc cgaaactgcc gggtgtagta cgcctttgtc
    22381 gcctcgatcc gctccggtgt cagtccctcg acaaatgcct ttccggcgtg gccgaagtgc
    22441 tgtttcaccg tcgccgaaac ggaaacgccg tcctgcacca ccgattctgc ggcacggcac
    22501 tcaatgtcaa tgacacggtt caccgcaccg gctccggctc cgctctggac aataggagat
    22561 tcgcccgtgg tcagaatgca cagcgaccat gtgggtgtgc ggtcaatgcc gcctgccttg
    22621 ttgccccgtc ctctgcccac gccctgtgcc agctggtaca cgtcgaaccg gctcttgccg
    22681 tggctgtcct tggacagctg cagctcgtcg atgcacaggg gaatgttgtg gagaaagccg
    22741 gcagtgcgtt cgtggctcac ctgcgtggcg ttgaacgtct gcacataccg ccccagcatc
    22801 gggtcgcccc agacgctcgc cgcaagcatc agccccacag tcttgcccgt gccggattcc
    22861 acgccccaga ggtgcacgaa aaacggcagt gctccgatat gccccagcag cacactggca
    22921 aaggaagccg ccagcaggat ctgtgccgtg cggctctcct gccggcagcg gacagcggtt
    22981 tcccgccaca cgtcatagct gccctgggga cgaattgccc ggtacatctc gctgtagttg
    23041 ctctcgccgt caaaggtcag gtcgggctgg tacggggaaa atacattgtc gtcccccaca
    23101 taccccagcc gtcccacact gtttcgctcc ggcagaatgt catagttccg gttttcgatg
    23161 tagcacagat atttcaccag tgccttggcg ttttccgagg tcactgccac gccgatggag
    23221 gacagctgtg tcaccttgtt cgccacaaac agcacgtcct tgccgtagac cgcataccgc
    23281 caccgtctgc ccttgcagaa ggcaattttc agcttttcct cgccggtgtc gatgttgatg
    23341 agccgttcca ccggcatgat cgggtgcggg caggcgatga tgggcatgcc ctccatgttc
    23401 tgctgcacgc cgtcctcgct gcacgtccac tcgccgccgt ccagttccag aggctggttc
    23461 gggaacgccg tggggttctg catattggga tcgccgccca cgttcatata ccgctgtgcc
    23521 ttggcgtagt tcttgtacac gctctggaac ttggtgaagc ccacggcgtt ggcattgtac
    23581 gccagctgtt ccagccgctg ggctttctgg aatgccccct ttgtctggaa cgcctcccga
    23641 tagggttctt ccgtgttgag gaagtcctcc cgcgtgtacc acagccgcag cgtcaggcgg
    23701 tgctcggcga tgtatgccgc aagcccctgc acggtcagcc gatagcagcg gtcgaagatg
    23761 tcctttttcc ccttgtcaat ggcggactgc cgcagtgcag cgatcgcccc gttgatcttc
    23821 tgggaatcat ccccgttcag cagcacgatg tccgacagca cctctgtcac cgcttcggca
    23881 gtgccgcagg aaaagttctg gtacttctcc ttctgctttt gaatgtaggt cagcatctcg
    23941 ctgtccatgg catcacttcc tttgattttg tacaatcctg tatcgccctt tcttggtgag
    24001 gatagggacg aatgccgccg atttttttcg ggtgtccttt ttgtataata ggctcccctg
    24061 taggggagct gtcaccgcag gtgac
//
LOCUS       DAWBGR010000013        23513 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_796_length_23513_cov_3.759272, whole genome shotgun sequence.
ACCESSION   DAWBGR010000013 DAWBGR010000000
VERSION     DAWBGR010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 23513)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23513)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23513
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_796_length_23513_cov_3.759272"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11792..11801
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18809..18818
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19438..19447
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5629 a         6312 c         5635 g         5907 t
ORIGIN      
        1 gcgttcccct tccgtccatg cttttattat acacaggaag ccagacagac gcttgtccgt
       61 tcaaaaacag tttacaggta ctcgccaaaa aaatccgctg cgaaatgatc ccgcagcgga
      121 ttttttcgtt ttttcctttg tatttatgcc aaatttccca ttttttcttg caaaagtgca
      181 ggaaactatg gtataataat tatatcaacg gacagctaaa gctgtcagac aggagcatga
      241 ctatgagtat acacaaacgc attccggctc tgctgctggg tgtgatcctg ctgtttgcag
      301 gaatccccgc aggcagaatt tctgcacagg cagcagacac aacgcaacag ctaaacaaca
      361 tcgttctgtt tgcccagttt ccagatgccg acacagacaa ctttatggca gacaagacgg
      421 acacggcgat cgccatttgc aacgacacca gcacaccgcg ttctctcacc agctatatcg
      481 acgccatttc ctatggcaag ctgcacgtga actcttattt cccgcagctt tccgacggcg
      541 tgatccagcc ctacattctt cagaattcca aagcggaata caccaactat gaacagtacg
      601 ccattgaaat ggtgcagaat atccggattc ctgacagcat accgctggac ggcaatcagg
      661 acggcatgac agacaacatc acactggtga tcgacggacg ggcagagtct atgggtgacc
      721 cgctttgggc aaaagctttc caggtgggcg gactgtatct tggagatacc cctgtaggca
      781 gcatcaacct gatgagcagc tacacactgc tcagttccac tattttcagc ggcgtgggaa
      841 cactctgcca tgagttcctg cacagtatcg gttatcccga tctctaccgc aaagacagtt
      901 cttccggcaa ccctgtggga cagtgggatc ttatggcaac caactccatc tttctgcaat
      961 accctctcgc ctatcagcgt tacgcggttt ccggctggct ggacgcgaaa actattacca
     1021 ctgccggcac ctacacatta cagccggcat cttccagcac aggcgatcgg ctctatctgc
     1081 tgaaaacgcc gttttcagac acggaatttt ttgcggtgga ataccgtatt cccggaaagc
     1141 agtactccga agaactggac tgcaagatct acggcgaagg catggtagtc tatcgtgtca
     1201 acactgccgt acagggcaac tatcgaagtg atactgacgg catctacgta ttccgacccg
     1261 atgaaaccac gctgaatgcc ggcggcggtg acctgacacg ttcctgctac ggcggcaaaa
     1321 acgcaccgga ttcgatcggt tccacagatc tggaaagcac ctttgcagac ggtgccttgg
     1381 tctattccga cggcaccaac agcggcatcg ctctccatga cattcagctg aacggtgacg
     1441 gcacattaag cttttccgcc gactttgccg atgtatccga ccggttgttg tggcagaccg
     1501 tggacagcgt gaactttccc gcagaccaga ccggctacga catggtgacc ggcgatgacg
     1561 gcaagctata tctgctgtcc gccaacaccg acggtgccac actgtactgt gtggaaaacg
     1621 acaccttaca gccagtcagc actgtgcttt ccggtactat gtacaacccg aagctggcat
     1681 tctccaacgg tacgccctac ctgctctggc aggacagcca gtatttcctg cacctgaacc
     1741 gctggaacag cacctccggt gtatggcaag attgctgcac cggcacagaa ctggctcaat
     1801 atgccgatat tgccgcagac agcagcggcg tgtacgtgac ctacactacc ggctcgttcc
     1861 cctatgttct tcacgcattc cggtttgaca atgccgccgg caccgtttcc ctgctgggag
     1921 atgtcattgc tgacaacgcc tgcaacatgg agatcgccgc tgcaaacggc agcattggca
     1981 tcggctaccg cgacctgaac gacaacaatg taccaaagct tgccgtctgg gacggaacag
     2041 catggaatac cacaacgctt tcggaacagg actgcggcac gatttcagtt ctggcagacg
     2101 gaaacagcat ctgggtcgtt ccctccggcg gcaagacaga cgttttccgc tgggaatccg
     2161 gcacggttac aaatattccc ctgccggaag ctgtccaagg acgggcattc cagatgacac
     2221 cggcagtggc acagggaaac ttctatatga cggtaaacgc ccagaatccg gaagaatttg
     2281 tcctttacgg attaaacaaa gacggcacat ggtcactgac cggcaacccc attgcacaga
     2341 gcatggtaaa ccagccggta ctggcagcac agaagcaggc tctctactgc ctttacgcca
     2401 cctctgactt gcagatgcag ctgaaaaagc tgacactgga aactgaaaca ccggcagtta
     2461 ccggcgatgt caacggggac ggtacagcaa atctggcaga tacagtgctg ctgcaaaaat
     2521 acctgctggg agaaactgcc ctgacagcag cacaggcaaa ggcagctgat ttgcaggcag
     2581 acgacagcat caacggtttc gatctggctg ttctgcgaca gcttctgaca aaggcggcat
     2641 aacatggatc tgaaacttct gctgcaatat ccgaaggcag tacaggtact tctggcagca
     2701 ctgcaaaaaa gccctgcttc tctgccgaaa ctggaaaaac tgctgccgcc ggaaatccct
     2761 gctgcggcac tgctctccgc catggaacag gcagggtttc tcaccaaaac cggcagccgg
     2821 tatgttttgc agcaggaagc actggagcag atgcagcagt ttttgcagct atacgccgct
     2881 gtgcagaacc aacaggcaga acaggacttc actcattttc tcgccgccca acgggaggca
     2941 gaaacacagc atgcaatgct tgtcacagaa cttgcctttt ttgaaagcgt ggtttctgga
     3001 aacagcgata ctgtgcatct gctgtacaca ccccttggcg gaaaaggcta cggcgagctg
     3061 agccgtgacc cgctgcgaaa tctgaagtat catctggtca tcaccatttc catgctgaca
     3121 cgctactgca ttcagggcgg catgccacag gaagaagcat tcaatctcag cgacctgtac
     3181 attcagcgaa cggacactgc cgtcagtgaa gcacagatcc atgtgctgca ctatcaggca
     3241 atacaggatt tcaccggcaa aatgcggcgg ctgcaagaca atacccgcta ttcgccgctg
     3301 gtgctgaaag ctatggacta catcagcatt cacctgcaca gccgtatctt tttacaggac
     3361 gttgccgatg ctgccgcagt gagtgcaccg cacttatccc gtctgtttca ggccgaagta
     3421 catcttagca tcagcgaata cattgccatt aaaaaagtag aagctgccaa gaacctgctc
     3481 cagtacaccg agcacactgc cgcctcgatc agcagcctgc tcagtttcag ctctcaaagc
     3541 tatttcatca aggtattcca aaaatacgtg ggcatgaccc cgaaagagta ccggaagcag
     3601 ttccgtgaca gcactcattt ttgataaggc aacagcataa aacaacagcc ccgcacttct
     3661 ccggaaagag aaatgcgggg ctgtttgctt ggaatcagca gttcagagaa gccgaaactg
     3721 cgggtttgga aatccgcgtg gaacaggtgc ggatttcctg taaaaagaaa tggagatgaa
     3781 taatgaagct tacttagttg gctttggctg tgctctgata ggtcacccat tcatagcttg
     3841 cagtcaaagg tgtcttgccg tcgaaaggct tcagccagtc gttgacacca attccgggcc
     3901 atacattcat catgatcttg ccggcagtgg acgggatatt gctggtagct gtgtagaccg
     3961 ccttgccgtc cacataccat gtaatgtaat ccttctgcca gtcgaatcca taagtgtgat
     4021 atccctctga tgcatcaaag cccagatcgt acatgtactc atgattgccg acaccattgg
     4081 tgtaatagtt gagctgtacc ttggtggtat ccttacccag aacctccaca tcgatctcat
     4141 cccacgggtt attctccgag gggccggtat aggtaaagaa cgaggaaacc acgccatcat
     4201 ttttgattgc cttcatggaa gtttcgtaat aaccatagtg atagtgctcc agagaacggt
     4261 actctgcacc agtatagtta tactgaccgg aactgtcctt gtcaatggtc agattcaaca
     4321 cgccgttgtc gaaagaagta ttgttctttg tccagccgca gtcaaaggga ttgccgtttg
     4381 tccagccgtc cgaagcttca aacagactgg tagcaccctt acggaaatct gccagtactt
     4441 ccgcatcggc actgggttca ttgcccttgg tgtcgtcctt gccggaagta ttgtcgtctt
     4501 tctgcaccag cttttgccgc agcactgcca gatcgaagcc gtttaccgta ttgtctgcct
     4561 gtacatctgc cgcctttgcc tgtgtttctg tgagagaaac ttctcccagc agatactttt
     4621 gcagcagtac aacatccgca gtgttgactg tctgatcctg atttacatcg ccggaaatga
     4681 tgtcatcatg tcccggtgtt cttgcggtgc tgttcatcgc cagcaggcag gtcagcggag
     4741 agttccagta gatcgtgatc tcattggtgg aatagctttc ccaatggtct acatagcact
     4801 ttgccgacgg agaatttgca cagtatgcct ttgcagcact gtcctccaga ccggaattga
     4861 caccgccgac cagcatacct ttcattgcct gattcttggc catagacggt ctgtggtgcg
     4921 ggtgttctgg tgaaaccgtg ccatagccag tcacgaagca ctctcccatt gcgttattgc
     4981 ccagcagata gtggagattg ctgcgggaag tgtccagata ggtgctgtcc tcagtcatct
     5041 ggtatgccag attcaggatc atgccggaag tggcaacgcc catgttgctg ccccagttat
     5101 atttggaaac cgacacccca taaggattgc tctgggaaaa acctttcatt ttgtcggctt
     5161 ctttcagaat ggaagtcttt gcacgggtgt agaggtcaga gttcgtatcc acaccgtcca
     5221 tagtgagaat ggcaatatta ccatagtctc ccacttccat ccagtccatg ccattctgca
     5281 cacgcatgct ttccaacgca gtacgatatg tgtcctcacc ggtagcacgc cacatctgtg
     5341 ctgctgccca gtagcgttcg tcaatatcgg aagtgtcgcc ataatcacca gtcgtaatgt
     5401 cagacggatt ggagaagata aagttgggat ttttttgcag gaatgcccac gcgttctttg
     5461 cagcattcag gcacttttcc gcaaagtcct tgtcgacctt ctggtagtat tcatatgcca
     5521 ttgccatcga cgcacagaaa tcggcagtcg ctgtggtgga aaccggcgtt acgatcagtt
     5581 cgtccgtttc cgtttccggc atcacataac cggggaaatt ctcgcagctg accttgtggt
     5641 gcacgccgcc ggtctggctg tcctgcattt tcagcatcca ttccagttca tagcgtacct
     5701 cgtccagcac gtccggaaca ccgttgccgc tttccggaat gccaatgctg tcgctgaaca
     5761 actccggatt ggcatcatag gcaatcagca agtctgccac agactttgca ccgggaacaa
     5821 catatctgcc gtagtcgccg gcatcatgcc agccgccgct gacatcgatt ttttcgttgg
     5881 tgtgataaac tgttgccagc gtgttatggc aagccacatg cccgaatgtg ctgtccttca
     5941 cctccgtgcc gcaccgctgg aggtacagca ttttcacgct gtcgtccagc agactgtcgt
     6001 aaacgttatt accaatggtg aacgaatacg actgatccag accgccgcag gtgatatagt
     6061 atgtaccagc ttctgtcacg gcggagaaat ctcccgtcca gttgttttca tcggcggaag
     6121 aataaaacgt cggagccgac agttccccgg tatagaccac ctgtccgctg tctgcgttga
     6181 cgacggaaaa actggtttca ctggtcacgt cgcggaacac agctgtcttg gtttcgtccg
     6241 gctgataacc tacctgatcg gtcatgatcg acggagcata aggcagaaca gaagtataat
     6301 ccacgttgga atcatctacc agttctacct ttacattgtc cagataaata gtatgtgccg
     6361 gcagatcttc ctcgttttcc agtccgcagt tgaacaccag cttggacatg acatctgaat
     6421 cctgcttcat ggtgaattca tagtccacgg tctgcggttc tgcggtcaga tcaagtcctt
     6481 tccaggtgta cgcctgatag gtgccgccat tctgctggat catggcttcc acataacggt
     6541 ctgtggtgga agagatctca tagctaacac ggtacacccc gtttttgtac aaggggataa
     6601 tatcatagct gcactgtaca gaatagttca gctttcccac agaatcaatt tgcagggcaa
     6661 gcttaccgtt ttccgtagtc agggacgcct ttccgccgct tgcctgatag gtgtcccagc
     6721 cgctggtgcc gctgtcaaat gtggagttgc tgaccagatt tttcacttcc gcagaagccg
     6781 gcatcgctgc cagactaccc atgctcaaca ctgccgcaca gagcactgcc agacattgcc
     6841 gaagtccttt gattcgcatc atactcattt cctctctttc tgaaaacagc atgccgctgt
     6901 ttctgtgtaa tttgtctgca cagaatgtat gaaaaacaat atcacatctt ctgtgcaaac
     6961 ggctttatgc gaacaattca ttatctttag tatatcacat ctttcacaag gataatacta
     7021 tttttcatga caaaagtagt aatttcatga tatgttatca aaaaagacct gacgagaaaa
     7081 tttcccgtca ggtctagtgt ttccctaagg caataccaca caaagattgc acgacagatg
     7141 cgtgcgggaa gatacgaaac aggctctgaa ggaaaatttg caagcagatg gcgtgcaaag
     7201 tcgccagacg ctctttgtgc ggtattgcct taactccacg tgttcctatg ccttttcata
     7261 taatgccagc atttgatgca gttcttcctg catttcgtcc accagactct gcggcgacag
     7321 cacctttacc catgccccct gcgacagcag gtacatgata atgccgcgtc cgtggttgac
     7381 ttccgcttcg atgatgctgg ttctgccgtt ttgctccact acctttgctg tgggcagtct
     7441 gtccagaatt gcctgtaccg acagaccgga aaaggcaaac cggattcgct ccgtcttccc
     7501 gggaaacaaa aactggttct tctcccgcag atcgccttcg tcaaagtcgt acttccgttc
     7561 cagcgtaaag tgttcctgat gctctgtcat cgacacaatg cggtctgccc gaaagaactt
     7621 cggcagatac tggtcatcgt caaatgcata cgcgatcagg taaaaataat actcactgaa
     7681 catcagcgaa accggtttaa ttttccgctt gacggcttcc cgattcattt tgtagtaagt
     7741 aatggtgatc ggtcgctttt catcaatgac ctgtatcagt ttccacagcg tatcgatcag
     7801 cgaattgcag tccgtcttga cttcgtgata atggtaaatt tccttgcgta ccagcttgtc
     7861 aaatttttcc cgatcctgtg ttgtggtaaa ccgtttcagc ttggaaatca gccgcagcac
     7921 gtcttccttg ggcaaacaac ggctgcccag cattaccttt accaatgcaa acaattcctt
     7981 atttttgagg aactcctcac agttcaggtg atacgcttta tctttatagg aatacaccag
     8041 ttccgcctgc tgcatcaggt cgcggtgttc cgccagaaat tcctgtattt ttccaatgtc
     8101 ccgtccgatg ctcttggtag acacgccgta ttccgctgcc actgctttta cagagatggt
     8161 ttctccccgc agcacccgaa agaaaagttc cagcatacgg ctgttttttt cagtttccat
     8221 gatgatgcct ccttttcttt cattatagca cagcagcggg acagcggtct gtctgttcaa
     8281 aaagaattca gaacgtattt acacaaaaat gagatgcacg tttttgcggc aaaattttat
     8341 gccagcggta ttgtcttaaa aatcacttcg cctgcaccat ttcgattgcc tttttcagct
     8401 gcgggtcttt ttccggattc ggatcgctgt agtccactgc atcttctccg gcttccacga
     8461 tctcgtccgg cttgatgcct accttgtgat aacactcccc cttggtggtc tgataagttg
     8521 ccaccgtcag tgctccgccg gaaggcatgg actgtgtcac ctgcatgata cccttgccga
     8581 atgtctgcgt tcccacaacg gaagcctttc cgaaatcccg cagcgaagcg gtgaacagtt
     8641 ctgctgcact ggcagtgttg ccgttgacga tcaccgtcat gggcaagctg cactctgtgg
     8701 catctgattc caccagcgtt ttggtcgtgc cgtcccgata ggtggcaatg gcgatctcgc
     8761 cctccggcag cagcggatcc aaaatctttt ccaaagccga caccagtccg ccgccgtcgt
     8821 cccgcaggtc aaatatcaac gcctttgccc cgtcctgtac cagccggtcg tagatcgcct
     8881 gaaactgttc cggcgtgttg cctttaaagg aactgatccg gatatagccg atctgtccgt
     8941 ccagcatctg tccctcggca gtcgtcactt cgatattctg ccgttccacg tccagttcca
     9001 gtgtgtcgcc gttccgctga atcgtcagct gcaccgtggt acctgcttcg cctcgaattt
     9061 tagacagtgc tccggcgtag cccagttctg ccagatcttc gccctctacc ttggtgagaa
     9121 tatcgccctg ctgcataccg gctttttctg ccggagaatc tgcattgact tcctccacct
     9181 gcaaatagcc ctcgtctgtt tgtgtcaccg ttacgccgat tcccacggtc tgaccagctt
     9241 cctctgtctg ataagcctga tattcctcac tggtcatgta ctgggaataa ggatcgtcca
     9301 gaccggcaac atagcctttc atagccccct gcataacggc ttcgtcgtcc acgtcggtat
     9361 aataattctg ctttacagtt gcggcaagct gccggagttc cctgtactcc gtgttgccag
     9421 cgtagttccg ataaatgccc agtcctgttc ctacggcaag cacaccgcaa agacagaaca
     9481 gcaggatcag tgtcgatttc cgcaccggca ctgttttttc ctttggcata tcctcctgca
     9541 ttgcatctga ttgttttcgc atataaccta caatcctttc ttccaaaaaa ggcagaggac
     9601 tgcaaacgca tacccctgcc tttttcattt tctcaaaaat gcattttgat tacagatact
     9661 tcatcgggtc caccttggtg ccgttgacac gcacttcaaa gtgcaggtgg aaaccagtgg
     9721 aaaagcctgt gcttcccaca tagccgatgg tctgtccggc actgacggtc tgtccggcag
     9781 aaacagccac cgatgccata tgtccgtaca acgtagaata tgtaccgtca tgctggataa
     9841 tgacatagtt accgtagccg ccgccgcagc cgcagctgcc gcttttcgga tagttgtggg
     9901 agcagccggt tacaacacga acgactgtgc cgccctttgc tgcaacgatc gctgcaccgg
     9961 cattgccgcc ggcaatatca attgcaccgt gtgtggtacc ccagcggttg ccgaaggtac
    10021 tggaaatggt atagaatccc gggcagggcc atgtgaagcc gccgccggac ggtgcaacac
    10081 tgccgctgtc gtcgctggaa gaatctgaac tgctgctgga agaactgttc gaactgctgg
    10141 agctgctgga attgtccttc tggctgttcg aagaagatgt attgtcattt ttcgaagaag
    10201 agctgctgcc ggaggactga ttcttcttgg cttcctcctg ctgctgttgc ttctgggctt
    10261 ccagcttctt cttggcttct tctgccgcag ctgcttcttc ccgcttcttc ttttcagctt
    10321 ccgcagccgc tgcaattgcc gccttgatct ggtcttcttc tgcgtccaga gccttgttgt
    10381 tttcttccag tgttgccaca tcgccgttca gctgctcttt ctgccgctgc atctgctcct
    10441 tggcttcatc ggtttcagaa taggtctgct ggagatcctc gattgccgcc ttgaattctt
    10501 ccttcttggc ctgctggtct tcctgctggc tttccagatc ggacttttct gtttccagtt
    10561 ccgccttctt tgcgttgcag gtttccagct tgtccttcag gtcgttgacc agctggttgt
    10621 catgatttgc cacacgggac atcagttcat acttggaaag caggtcgtag aagtcagtcg
    10681 cacccaccag agccgaagcc agactgtcgt tgccctgtac gtacatggca cgaagccgca
    10741 tcttgaactc gtccagtcca atggcaatat cggcttccat gaccttgatg tcatcttccg
    10801 cctgtgaaat tgcctgtgtg gtttcgtcga tagactgagc aatgcgatcc agttcctgtg
    10861 aaacgatgtc cagattgctc tgctgcaggg tgatcttttc ctgcaaagtc ttctggtacg
    10921 cttccttttc ttccagatca gactgcaaag aatccagttc tttctgcttc tctgcgatct
    10981 tcttttcgtt gctcttgcgt tcttcctgta attctgccag cgtctttgcc gataccggaa
    11041 tatccctgat cggtgtccag tttgctgcca tgccgcctgt cagaatggtc atacaaagca
    11101 acgcactggt aaaacgcaga ctcagatgtt tcttcttact catagaaaat agcctcctaa
    11161 gatgtgcatc ggcattgttt taaacattca ggtgcttccg catagagatc atactgccga
    11221 acgctccgaa cagcgtaccg gacagcagat atgccagagc caccttccat gccagtgcac
    11281 ggaacggaat gatgctgccc atgccgatca cagacaggaa ttccacctgt ccggtcagca
    11341 ggccgaccag cgaatcatag ccgaaccaag tgatcgccag tgcaacacca ccagcaagca
    11401 agccggtgat cataccctcc acaaaaaacg ggatacggat aaaagagttg gttgcaccaa
    11461 cgtatttcat gatctgaatt tcttcccgcc gtgcgaaaac gctcgccttt gtggcattgg
    11521 aaacgatcac aacggaaatg acgaccaatg ctgcaatgat cacaccgaat acaacagtca
    11581 cgatcttccg tacctcggtc agaacgttga caaagtccat gggagcacgg atcgaatcaa
    11641 tgtgatccag tccctctacc tgtgtaatcg ttgcagaaat ctgtgaaata tcttcaacac
    11701 ggatccgata gctgtcaatc agcgggttgt cgtcccccag tgaatcgaac agcacttcat
    11761 aatccgacat gctgtttttc agatcttcaa annnnnnnnn ncgcctgctc cttggagtaa
    11821 aaagaaatct ccgaaatgtt tggaatctgt gccaattccg attgaaaatc tgcaagttcc
    11881 tgatctgaag taccctcgtc catatagata atgacttcat ttttatcttc tacgccgccg
    11941 atcatagagg tcaggttcat ataaaacaac accgacatac ccaccagcag cagtgaaacc
    12001 aacagcacac agaatgaagc gaatgccatc atccggtttt tccagatgtt ttccacaccc
    12061 tgtccaatca ggtacttgaa accactagct ttcatggatc agattccctc caatcacttc
    12121 gtcaaagacg atctgtccct gatcgatatt gatgatccgt ccgccgaact gccgcaccag
    12181 actgtgttca tgggttacca tcagaatggt agtcccgatg tcattgatgc cctgcagcaa
    12241 ttctacgatc tcataagaaa gttccggatc gatgtttccc gtcggctcgt ccgcaataat
    12301 cagctgcggg tcgttgacca atgcacgggc aatcgccaca cgctgctttt caccgccgga
    12361 cagctcgtcc ggataggcat atgtcttatc ctgcaaacct accagaccaa ttacatacgg
    12421 cacacgcttc cggatatatt tcgtcggtgc atcgattacc cgcagcacga atgccacgtt
    12481 ttcatacact gtcatagacg gaatcagtcg gaagtcctga aagacaaatc caatggtacg
    12541 ccgcagggca ggaatccgcc gccgccgaag tttgttcagg ttatagccgt tgacactgat
    12601 cgaaccgctg gtcgggttga tctcttttaa gatcagtttg atcatggtgc tttttccggc
    12661 accggacgca ccaactacaa aggcaaagtc tccgtcattg attttcaagt tgacattgtg
    12721 cagtgcatct acgccgctgg agtatgtcac agacacgttc ttcagttcaa ccatagttca
    12781 cacacatcct ttctctgggc acttccggca taccgccgga aataccgttt cagttgatcg
    12841 cagacatcca cttttccgga cagcacacac aagtttcctg cgttgctgcc cttaccaata
    12901 aaattgcaca aagagccgcc gatgcaaaac gccacacgct gcaccggcgg cttttatgcc
    12961 ggagcgatgc ttcacgccct caccccgtcc ttattcatca gaacttcgcc ggattcgcag
    13021 agaggtactt cttgaccatc aatgcgatct tgaagataat ggcatggtcg aattcccgca
    13081 ggtccagacc agtcagcttc ttgatctttt ccagacggta caccagtgtg ttccggtgta
    13141 caaacagctt tctggaagtt tcagaaacat tcaggttgtt ctcgaagaac ttctgaatgg
    13201 taaacagcgt ttcatgatcc agagattcaa tgctaccccg cttgaatacc tcgtgaagga
    13261 atgtttcaca cagcgtggtc ggcaggtgat agatcaggcg ggcaatgccc agattctcat
    13321 agctgacaat cgccttgtcg gtatcgaata ccttgccgac ttccagagcc atctgtgctt
    13381 ccttgaacga acgggacagt tccttgacac cggtcacaat ggtaccaata cccacgttca
    13441 cgcgggtgta gaactcgctg ctcaaagtat ctgcaatgga acgggcaagc ttttccagat
    13501 ccttggtgtc cacagcaccc ttgatctcct tgaccagaac aatatccgat tcggaaatgg
    13561 taaagacaaa gtctttattc ttatccggaa acagattctg aatgacatcg tatgcagaaa
    13621 cgtcatttgc cgatacaatg cgtaccagca gcactacgcg gctgacttcc gagttgaagt
    13681 gcagttcacg tgccttaatg acgatgtccc ccggcagtac gttgtccagt actacattct
    13741 tgatgaagtt gttgcgatcg tacttttcat cgtagtactg cttgatattg aacagggaaa
    13801 ttgccagaat attggcatac ttcgcagcag tgtcgtccat gccctctaca aagaccgcat
    13861 actccggctt tgcacgagaa ccaaacggct tataggtata accgtcgcgg ataaaaatat
    13921 catgtacatc actgaggtcg agggaaacga actcattggt cgttcccacc tttgtcaggt
    13981 cgctgcaagc gatgatggtc gcggtgtcat cgacaacgcc aacggttgcg tcaatggtat
    14041 cccgcatctg gtgaaccagt ccttgaaata atctgttcga catatggaac ttcctttcct
    14101 tttgtaatct cggcttccgc cagttttgcg gccggcgggc atatcacaaa ctgcatccac
    14161 ctgttcaata tattacaaat tgtaacacat tgcgaaacga tgtttgtgaa tatgcagtta
    14221 aactttatga gatatttcga gtattgctac gtttttttgt gctttcgcac gattttccat
    14281 agagaatcta tttttttgct gtaacctgct tgtaatcttt cagcaggatc acatcttctc
    14341 cggacaggtc accttcagga tccggagggt attggcaacg ccctgacgag ccgcctcaca
    14401 gagcagcaga cgtgcctgct tcagttccgg cgtttccgca tacttgatac tgcacacgtc
    14461 atagaacttg tggaacagag tcgccagctc ggtagcatac ttagtgacct tggacgggtc
    14521 atatgccttg gcagcagcgt ccagttccga cggcagtgct gccagatgcc ggatcagttc
    14581 cagttcctgt ggctgtgtca gcagggacag gttgctgtgg tcgcactgtt ccagtgtcag
    14641 tccttcctcc gccatggcac gcagcaggct gcaaatgcgg gcatgggcat actgtacata
    14701 gtataccgga ttctgcgagg actgctccac agccagatcc aagtcaaatt caaaatgaga
    14761 atttgcctct ctggtgttga agaagaaacg ggctgcgtcc agcggaattt cgtccagcag
    14821 agtgaccagt gtgatcgcct tgccgctgcg tttggaaagc ttgaccactt cgccgtcacg
    14881 catcagcatg accatctgca tcagtaccac atccagacgg gctgcatctg cacccagtgc
    14941 ggtcagggct gctttcagac gaggcacata gccgtggtgg tctgcaccca gaatgtcaat
    15001 tgccttgtca aacttccgca ccatcagctt attatagtgg taagcaatgt ccggtaccac
    15061 ataagtgggg atcccgttgg cacgtaccag cacgaagtcc ttgtccgcac cgaaatccgt
    15121 tgccttgaac cagacggcac cctcctgctc ataggtatag ccacgcttgg tcagttcgtc
    15181 taccacccac tttacctgtc cattctgatg cagggtgctt tcgcggaacc agttgtcata
    15241 gacaatgcgg tacttcttca gatcccgttc cagaccggcg atgttcttcg gcagtgcaaa
    15301 tgccaccagt gcgtcccgtc tggtcttggc atcggtgttg acatacttgt caccgttttc
    15361 agcgataaag ttttccgcat gctctgtaat gtctgccccg tgataggcat cttccggcat
    15421 ttccacagag gggtcaaagt gctggagata ccgcacttcc agagaagtgg caaacttttc
    15481 gatctggttg ccggcatcat tgacatagaa ttcccgctgt acgtcccagc ctgtccacgc
    15541 cagaatctct gcaaggctgt cgcccagtgc accgccgcgg gcattgccca cgtgcatcgg
    15601 tccggtgggg ttcgcggaaa cgaattccac tagtgcccgc tttccggcac cggtctgcgt
    15661 cttaccgtaa ttttcttttt ccgtgaggac agtttccgtg acatcggcaa accacttctg
    15721 ctggaggaag aagttcataa agcccggacc tgcgatctct gcccgtgcaa agatcgtgcc
    15781 gttcagcgaa atagcctcca gaagcagctc cgcaattttc cgcggtgcca tgcggaaggt
    15841 ctttgcactg accattgcca catttgccgc aaagtccccg tgggacttat ctgccggaat
    15901 ctctacccga aacgccggga tcggttctgc cggaagcttt ccctcgccta cgagccgtcc
    15961 cagtccctcc accagaatct ggtgcagagc ctcggaggca ctcttcatac aatctgccat
    16021 agtttacttt gttcctttcg tattctcctg cggttcctgc cgtttccagt gaatggtgat
    16081 cgtgttttcc gacaggaaat ttgcattcat atcaataatg tattccattt ccacagaacc
    16141 ggaggattcc tccagttcgc agagcagccg cttcgcctgt acgccgatag tcattgcccc
    16201 gtatggagtc tgatacaggc agaaatgctt tctgccggtt tccagcgtca ggttggaatt
    16261 tgccgtgccg gtgcgtaaga tcgtcaccag tgactcgttc gccacctgta tggtggtcgt
    16321 cgagccggca aagcccgttg cgtctgtatc ttcataggac aactgcaacg tgtcgtcccg
    16381 catctcacag gtaccggtgg tcatcagttc ggtttcttcg tcaccgtccg gtgtgatctg
    16441 ccgtgttttc atggtaatca gaacagattc caaaaatacc tgctcccttc ttagaaattg
    16501 cagcggaaca cctttcccac cacattccgg tgcagaaaat gtcctgcatt ttcaatgaac
    16561 atctccgtgc tgtcgctgac ataataggtg ttttcgccgg cagtctgccg gtctgtcagc
    16621 agatcctgtt cttccagaca acggcgggca tacttcgccg cctccgctcc ggaggagatc
    16681 agctgcacat ccggtcccat ataatccgcg atgatgtcac ggatcaccgg ataatgggtg
    16741 caacccaaga tcagggtatc cacgccggct ttccgcaccg gttccagata ctcctctgcc
    16801 accagccgtg tcaccggatt gtgaaaatcc gtatagccgt tttccaccag atgcacgaac
    16861 agcggacaag cctgcccgaa tgctgtcatt tccggatgca gcttctgaat cgccttttca
    16921 tagctgccgc tgcgaatggt cgccggtgtt ccgatgatgc cgatcttgcc gttgcgtgtc
    16981 acttcgcagg cagtccttgc cgccggaagc accacgccgg taaacggcat atctgttaca
    17041 aaaggctggt cgcctacaat agagctgact gtaccgcagg cggcaatgat cattttcacg
    17101 gcatactgcc gcagaaacgc cacgtcctcc ttggcatagc ggagcaccgt ttccggactg
    17161 cggctgccgt agggaatgcg ggcggtgtcg ccgaaataga taatattttc atggggcaga
    17221 atggcgttca gttctttcac tgccgtcaga ccgcccatac cggaatcaaa aacgccgatt
    17281 gcatcatttt tcatggtgtc atctctgctg ctcattgtcc ggagcagcgt ctcctttcct
    17341 taacgactct aaggcaaccc tgcacaagct ttgtgcggtg ttgccctaac tatattcagg
    17401 ttatcgttca ggttcttgca gcgttttcta ttgcctgctc aatgagttta tctaccagat
    17461 attcctgctt catgcccaga tgttccatca gcttcggata catgctgatg ctggtgaagc
    17521 ccggcagcgt gttgatctcg ttcagaatga tgctgccatc cttagagagg aagaagtcca
    17581 cacgggacag ccccttacag cccagtgcct gatatgcccg cagagcagtt tcccgaatcc
    17641 ggtcagattc ctgatcggtg atccgtgccg gaatgtacag cttggaggaa ccgttgacat
    17701 acttgtcgtc gtagtcgtag aattccgcag ccggctcgat ctcgcccaca taagaagcaa
    17761 tgggagcatc gtagccgaac acagccactt ccagctctct gccgtcgatg aattcttcga
    17821 tgatgacttt ttcatcatgg gtgaacgcga tctgcaccgc atctttcagg gattccagat
    17881 cggtcgcctt gctgacacct acagaggagc ctgcatttgc cggcttgacg aacagcggaa
    17941 agcccagtgt ttccgcaatg gcaacgcact tttcgttcag cttgttcagg tcacgctggt
    18001 gtacgcttgc ccacttggca gtctgaatgc cgttgtagtc cagcaccgta tgggtcatgc
    18061 ccttatccat gcacacagca ctgctcagaa cgccgcagcc cacatagggg attcttgcca
    18121 tttccatgat gccctgtaat ctgccgtcct cgccgtttct accgtgcagc accggaaaga
    18181 ccacgtctac cttttttata taagtttccc cgttttcaat ggtgacgatg ccgccgtgca
    18241 gcggatccgg agagaggatc gccgcagtgc agtccggatt ctgttcccat gcaccagtag
    18301 agatctccga aacgtcgccg ggaaaataga gccagcggcc tttcttggtg ataccgatgc
    18361 acagtacctc atacttttct ttcggaatgc tgcgaatgac gttctccgcg gaaaccagcg
    18421 aaacatcatg ctcactggat acgccgccga acaaaacggc tactctgatt tttgccatgt
    18481 gtctatactc ctttatgatt tgtgagagcc tgcttcgcaa aagcaaagcg aacccaaatc
    18541 ctgtcgctgc aagaaccggt gtcatgccgg atactattat atatccggat acagcggatt
    18601 ttagaactga tctgccgcca aattctgtat acaaaaccgg caaaacagac agcagcgaag
    18661 attcctcatt ctctatttta tcataatcta caaaaatctg caaggggttt tcaaaaaaaa
    18721 gtgaatctga tataatgtta acaattatat gattaagaca aaaacaacag acagaatgga
    18781 tatgaatatg gaaaaaacga agaaagccnn nnnnnnnnaa aaaaagctgt cccgaatccg
    18841 tttttttctc aaaaaaggaa atttttttga gaaaagcctt gacagaaaac cggaaatgtg
    18901 ctatactgga atcacctcat tgcatgaggc ttattttttg cacaaattcc ggacttgctg
    18961 tccggtttgc tgcgaaaacc ctcatgcgga ttctgaggga ggcaaaagaa ccggaagact
    19021 gctgatgaca gcaggagagc cggcttccgc atatcggaag aagtaaacga gcaggaggtg
    19081 ccgatcatga gagtgaaagt aacactggct tgcacagaat gcaagcgtcg caactatgtt
    19141 accaagaaga acaagcggaa caatccggac cggattgaaa tgaacaaaca ctgcaagttc
    19201 tgccggaagc atacgctgca cagagagaca aagtaatggg ggtccgaggc aatggctgat
    19261 aaggaaaaaa acgccgggca ggaagctgct gtaaagaagg cgaacaaagc agcatccgca
    19321 gactccaaaa aatccggcaa aaacaaaaaa ggcgataagg aaaagaaggg cttcttctcc
    19381 ttgggggcaa aggcgggtcc ctccggccgg ggctgcggct gcttggcggg tggctcgnnn
    19441 nnnnnnnatt caaaaaggtc gtttggccgg atcgcaagaa ggttttcaac aacacgctgg
    19501 tcgtagtcgt tgttgttgtc atcggttctg tgctggtagg tctgatcgat tccggtctgc
    19561 tgaagctgat gcagttcctg atgggacttt ctaactaatt tcgtgaccag aacaaggagg
    19621 atttcgtatg tcagaggcag ctaaatggta catcattcac acttattccg gctatgagaa
    19681 caaggtcaag caggacattg aaaaggtggt tgaaaaccgg aagcttcatg accttatcct
    19741 agaagttcag gtccccaccg aaacggtgac agaaatcaca gacggaaaaa caaaagaact
    19801 ggaacgtaag ctgtttccgg gctatgtttt tctgaaaatg gtttacacag atgatacatg
    19861 gcatgtggta aaaaatatcc gcggtgccac cggttttgtc ggtcccggct ctgagcccac
    19921 tcccctctct gaggaagaag tggcaaagat gggcgttgac atcggcggca acatcgaagt
    19981 gaactacgct gtcggtgata ccgtaacgat ctccggaacc gctatggacg gcttctccgg
    20041 cgtggtacag gccattcatc tggaagacgg tacagttgat gtgctgatct caatgttcgg
    20101 tcgtgaaact gccgcaacat tgcagctcaa tcaggtgatc cgtcaggacg attaattgca
    20161 aacatgcaaa aacaattcgg aaccgcccgc ggttccggtg ggagagatgt gacagcatct
    20221 cgccattaac cacaatatgg aggtgtgcag tatggcacag aaggtaacag gttttattaa
    20281 gcttcagatt cccgcaggta aagcaacacc tgctcctccg gttggcccgg cactgggtca
    20341 gcacggtgta aatattatgg cgttcacaaa ggaattcaac gaacgtacaa agaatgacat
    20401 cggtatgatc attccggttg ttatcactgt atacgcagac cgttcgttta cctttatcac
    20461 caagactccg ccggctgctg ttctgatcaa gaaggcttgc ggaatcgaca ctgcttccgg
    20521 tgagccgaac aagaacaagg tcgcaaacat caccaaggaa caggtacaga agatcgcaga
    20581 acagaagatg ccggacctga acgctggttc tctggaagct gctatgagca tgatcgctgg
    20641 tactgcccgt tcgatgggta tcgttgtcgt tgactaacgg gagccgaacg tcggtgggag
    20701 gaaatttccg ctaaccggat ctgcgagaaa gcaatccggt actaccacta cactaggagg
    20761 attaaaatat catgaaacac ggaaaaaaat ataatgacag ccgcaaggct gtagatagtg
    20821 caaagcagta cgcttctgaa gaagcactgt ctttggtttg cacaaatgca aaggctaagt
    20881 tcgatgaaac catcgaagct cacattcgtc tgggcgttga ctcccgtcac gcagaccagc
    20941 aggttcgtgg tgcagttgta ctgccgaacg gcactggtaa gaaggttcgc gtttgcgtat
    21001 tctgcaagga aaacaagtac gaagcagcac aggctgccgg tgcagactat gtcggcggtc
    21061 aggatctggt agacaagatc accaaggaaa actggatgga ctttgacgta gttatcgctt
    21121 ctccggacat gatgggtctg gttggtcgtc tgggtaaggt cctcggtccc cgcggcttga
    21181 tgccgaaccc gaaggctggt accgtaacac cggacgttgc taaggcagtt accgaagcaa
    21241 aggctggtaa gatcgagtat cgtctggaca agaccaacat cattcactgc ccgatcggca
    21301 aggcttcttt cggtgcagat aagctgactc agaacttcga aactctgatg gaagcaatcg
    21361 tcaaggcaaa gccggctgca gcaaagggta cctacctgaa gtcctgcgtc gttacttcta
    21421 ccatgggcgt tggcgtaaga gttgctacca gcaagtatgg tgtctgagtg ctctctttga
    21481 gaatctcaaa gttttttgca atttcttccc tgctgtcgtt tccggcggca gggatttttg
    21541 cgtttcatct gcatctcgaa aaaaattttg atttttttgc aaaaaaggct tgacaagcac
    21601 cgaaagatgt gctatactgt aatagttgtc aaacaacaat atcgtattct aaagacagtt
    21661 ggtggcgtaa gccgtaagtc caaccgagga atgtgcaatg acctgtgaaa tacagtgcac
    21721 ggttccctgt cgaatcgtgc ttttttattg cgatttctga tacgatacat gatctatcgg
    21781 aggtgaaatc tcatggcaag tgaaaagatt ttggaaagca agaaggctaa ggttgctgct
    21841 ctgacagaaa tgctgcagga cagtgtatct ttcgttctgg tagactacaa gggcatcagc
    21901 gtagaagatg acaccaagct gcgtaaggaa ctgcgtgaag ctggcgtaca ctatgtagtt
    21961 gaaaagaact ccatgctgcg ttttgcattc aagaacatgg gcatcgatgc atttgacgaa
    22021 gttctgcacg gtacaactgc tgttgcactg tccaaggacg atcagactgc tccggctcgc
    22081 gtactgggca agttcgctgc agatcacgaa gacttcttca atctgaaggc tggttacgta
    22141 gaaggcgttg cttatgacgc ttccggcgta gaaaagctgt ccaagattcc gtcgaaggaa
    22201 gttctgctgg cacagctggt tggttccctg cagggtccaa tccagaagct ggctgctaca
    22261 ctgcaggcag ttgtggacag caagtccgaa gatactgctg cataagtatc gcagagattt
    22321 tggctctgca aaatgtttta tttaattgtt aaaggagatt attatcatgg catctgaaaa
    22381 ggttttgaag ttcgttgaag atatcaagga actgtctgtt ctggaactgg ctgaactggt
    22441 tgacgcaatt caggaagaat ttggtgtaac tgctgctgtt gcagctgctc cgactggcgg
    22501 cgacaacggt gctaacgctg ctgctgaaaa gactgaattc gacgttgtta tgacttcctt
    22561 cggcgacgct aagatggctg tcatcaaggc tgctaaggaa gttctgggtc tgggtctgaa
    22621 ggaagctaag actctggtag agtctgctcc ggctactctg aaggaagctt gcccgaaggc
    22681 tgacgctgaa gctctgaagg ctaagctgga agaagctggt gcaaccatcg aactgaagta
    22741 agttttttcc gacatttctt cgtttcataa aaagcgggga catctccatg tgaggtgtcc
    22801 ccgctttttt attgctttca aagcgtaaag tgcagcacta ccatgagaat cacccccggc
    22861 acgcccagca cccctgcctg taccagatgc agcagattca atgcgggaga aaatccgaag
    22921 aatcccccgc cgtagtgcac cagcagcaat gccagaaagc cgcttccgac tcctgccagc
    22981 attgacaaaa tctttcgctt tcgccggaga taatagcata gcatgcaaat tcccaacaca
    23041 gcccagattc cccagaatac cacttcttcc atctttcttc tccttttatg tgagcaaata
    23101 cgccagagcc attagcaatt tcttgtcgca gccgtcccag ccgccgtctt tgtacagcat
    23161 gaccatcatg gcgagaatca acatggtgtc accgtccagt ccggacaaga gccccttggg
    23221 gcgaaactgt gccagatttc ccagcagcgg cataccagtc tgcggcgacg gtgttttttc
    23281 ctgcggcggc tcctccggcg gatagtccgc atagggagag ggttcctgct gtttgccgaa
    23341 atacacccgc ttctgcattt ccctggcacg ccgtgccgcc tcccgccgca ttgcgttctg
    23401 ctcctgttga gaaaattgtg ccatgtgatt ccctcctctc ctgccaaaac ctgtttcctt
    23461 ttaaggcaat accgcacaaa gattgtgcga cagatgcgtc gtcaaatttt tcg
//
LOCUS       DAWBGR010000014        23400 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_799_length_23400_cov_2.828571, whole genome shotgun sequence.
ACCESSION   DAWBGR010000014 DAWBGR010000000
VERSION     DAWBGR010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 23400)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23400)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23400
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_799_length_23400_cov_2.828571"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1107..1116
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6101..6110
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7743..7768
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9442..9451
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11005..11014
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14929..14938
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19827..19836
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8039 a         4530 c         4502 g         6243 t
ORIGIN      
        1 tgctgggcat tgtcatgatt ccggttctgg tcggcatgat gaacattacc accggttaat
       61 tttactgtga tggtaaatca ctcaaaggag gaacagagca tgaaaataat gtcctctgtt
      121 ccttttttgg cattatgaaa aattattcaa aaaaggaggg atttcatggg cgttgtttct
      181 gattttgtgg atggcattga aagtgctgtc aaggactttc taaacgatgc tgtcacacaa
      241 acattgaaaa caacattcga gctgctccaa ggcggattgg ataccagtaa agcagatggc
      301 attggcggaa tgtttcagca atttcttagt gttcatcctg caacctttac gggcggcgga
      361 agcactggtg caactgctat ctggacaaca attgaaaagg tttgcaatga tgcagtagta
      421 cctgttgctg gcatggtatt tgcaattgtc gtcataaatg acttaatgca aatggtcatg
      481 aacaacaata acttcaagga catagatgac agtatttttg ttcgctggat attgaaagta
      541 gtttgcggag tgcttttaat ctctaacaca tactatatcg cctccggctt ttttgcattt
      601 ggtacaaaag cgaccgcaac agctatgaca acactactcg gcacaaatgc ttttagcggc
      661 aatatctcag ttgcatctga ttacggtatc ggaatgctca ttgtcctact gttattgtcg
      721 ctgattatct tagtaggcgt ctttgttatg cttgcagtca ttgtagttgt actagcatca
      781 cgtatgatag aaatttttat gtacctcgga atttcccctc tcccaatggc tacgatgatg
      841 ggaggacaat ggtcagaagt aggaaagaac tggtttcgag gacttgttgc actggctttt
      901 cagggcttat tcattatcct tgccttaggg atattctcca caatgtttac gaatgccctt
      961 gcaaagttat cttccggagg ctctgcaatc atgcagatgg caatcttatt ggggtatacc
     1021 ctcgctttga tctttaccat tctgcggtct ggtgcaatat ccaaatctgt ttttaacgct
     1081 cattaacttt tatcttttcc ctactgnnnn nnnnnntttt ttcttgtgcg atcatttgta
     1141 gggttgggcg gcggtattgt tactggcggc attcttgcct cacctgctat tttctgtgga
     1201 ctatacagca aaaacggact gcatctggaa cagcaagtga aattgacact tgacttcttt
     1261 cgcagcccca gaaaacgaac ctatcaatct gaaaatgttt accaacaaat caaagatgca
     1321 atagaatatc agcacttacg cacagttttg cgatataaca cgtatgaaat tccaaaaaga
     1381 aagggaggca atcaccgtgc aaaaaaacaa aaagcagata aagaactcca aagcaccaac
     1441 gcataaagcc gttccggcaa cgccgatgac aaaacaagag agaaaagcaa tagaagccag
     1501 aatggcaaaa ttgaaaagca gcaagaaaag cagtacacaa aagacaattc cttatctgga
     1561 aatgtttcag gacggtactt gccagataac aaattccact tactcgaaaa ccatacagtt
     1621 ttttgacatc aattatcgac ttgctacttt tgacgaaaag aattttatct tctccaagta
     1681 ttgtgatctc ttaaattatt tcgataacac tgtaaaattc caattgacat ttgaaaatca
     1741 aaacactgat gtcgatgaac ttctgaaaat gttggaaatt ccggaacaaa ctgacgactt
     1801 caacgatatt cggcaagaat actccgctat gttgaaaagc caattgattt ccggttcaaa
     1861 cggtcgtgtg cttcgaaagt ttttgacatt tacagtagaa gctagctcgc tgcacaacgc
     1921 ccgtttgaaa ttggacagta tcgcagcaga ggtacaaaac attctgcgtt ccatgggcgt
     1981 tgcaagtaca attttagatg gcaaagaacg attagaagtg ctctaccgtt cattgaatcc
     2041 gttttcagat gaccccttcc ttttcgattg gaaatatcga attaagtctg gtgcatctac
     2101 caaggatttt attgcaccta ccagtcttag gtttgtaaaa tcaaattttg aaatcggaga
     2161 aaatggattt ggttcagttt attctatcaa cttgcttgcc ggagaactgt ctgatgaggt
     2221 tttatatgac tatctttcca atgacgatct gatatgcgtc aatctccatg tagagcctta
     2281 cgatcaaatt gcagcattga aatttatcaa aggcaagctt tccgacgtgg aaaagatgaa
     2341 aattgacgaa cagaaaaaag caacgcaatc tggatatgat ccgaatatcc tgccgccgtc
     2401 tgttaagctt tatatcgatc agctggaaaa aatgctggac gacttaaata gcaagaatga
     2461 acgtctgttc cacatcactt taaccattcg ggcatatgca gaatccaaaa agaaaatgaa
     2521 acttcaacag gatttgctaa aacgaattac gcagaaaaac agctgcaaat tgttttcatt
     2581 ggactatgag caagaacaag gtctttgctc cagtttgccg ttgggttata acaatgtcaa
     2641 agtgaatcgt gtattgacaa cttcggctat tgctgtattt gtgccattca cgacacagga
     2701 gttgtttcag ggtactgacg cttcatatta tggcttaaat tcactttcgc agaatatgat
     2761 tatgggaaat cgaaaattac tgaaaaatcc aaatggattg attcttggaa cgccgggttc
     2821 tggtaagtct ttttctgtaa aacgtgagat tttagataca ttcctaaaaa caacagacga
     2881 cattatcatc tgtgacccag aagccgaata ttaccctctg gtacaagctc ttgacggaca
     2941 gctgattaaa atttctacaa gttctcaaca gcacatcaat cctatggata ttgtctggga
     3001 caatgaagaa gacgacgaaa atccaattgc agtaaaatcc gactttttga tttctcttat
     3061 ggaattgatt gttggcggca aatatggact aaccgcagaa gaacgttcta ttgtcgatca
     3121 atgtgtgaga aatatctata accactttct ttctcataat ccttctaagg aaaccatgcc
     3181 gattttagaa gatcttctca aagaactgcg tgaaaatggc ggagatattg caaagcgtgt
     3241 tgcaaattcg ctagagatgt acgttacggg atcacaaaac gtgtttaacc accgtacaac
     3301 tgtggactta aataaccgca tcatttgttt tgacattaag cagctcggca atcagctaag
     3361 aaaagtcggt atgctgattg tacaagatca agtatggaac agagtttcct tgaaccgtgg
     3421 aaacaaatca actcgatatt acatcgatga atttcacttg ctcttgaaag aagaacaaac
     3481 tgcaaaatat tctgtagaaa tgtggaaacg tttccgaaaa tggggcggta ttcctacagg
     3541 cattacacag aatatcaagg acttgctgca atcacaggaa attgaaaata ttttcgataa
     3601 cagtgacttc atttatatgt tgaatcaggc tgccggcgac agagatattc ttgcagaaaa
     3661 gctgcatatc tctgaccagc agttaaaata tgttacaaat gccggacgtg gacagggact
     3721 tattttcttt ggggatacaa tcctaccctt cgtagacaga ttcccaagag atacaaagat
     3781 gtatcagctt atgacaacag atccagccga tcttgcaaac aaagtttcat aactcaattt
     3841 agtgtcaaag gtggtgaaaa aaatgagaat gcacaaacgt aggcaagtag gatttcaatc
     3901 tgggagagat tttcagctac aggaaaaaaa gcacgtccat gttcattttt ctcagcaagt
     3961 ggacgactcc ggaaagctaa aaacaggtgt acaaattaca cgccagcaaa atcaagaaat
     4021 tgggaaatat cacggcgttt ttcatcgtgc tgcttctata cagcatcaca tcaccggaga
     4081 tgttcctaag attttccctg acaataccgt tgtatctgct gcaaagaatg ccatgaaatt
     4141 tacaggacat actgttctag cagcggaaaa tgctgtgatt cgtcgaaaag acccagctca
     4201 tgcagataat cggcattggc atttgcaatt tcaacgtatg caagattcta ctggaaatca
     4261 gcatacacaa gttcatttca tccgaaaaga aaatgaaaac ctcggaaaca cacatggctt
     4321 attccatcgt gcagcttcct tgcagcatcg tattaccgga gatactccta agatcttctc
     4381 tgatagtgct gctgtatctg ctgcgaagaa tgccatgaag tttacaggac acaccattct
     4441 ggcagctgaa aatgctgtac tgcgacgtac tagaccagaa tacgctgata accgacattg
     4501 gcatctgcaa tttcaacgca atgcagacag cagcggaaaa ttgcatacac aggcgaggtt
     4561 tgttcgtata gaaaatccta cagtcggtga atatcacggt gtattacatc gcattaactc
     4621 tatgcatcac cgtatcactg gtgatacgcc aacgatactg cctgattcag ttgcctttca
     4681 aactgcaaaa aaagcacttc gatttacagg acatacagca ttagctgtag aaaacgcttt
     4741 tatacggcgt tttgatgctg gtcatgctga taatcgacat tggcaattgc aattgaaacg
     4801 aacacaagat caaaatggtg gatttcacac taaaatgcac tttgtgcgta cagatgttcc
     4861 gcaattgcaa aaaagtcgtg gtgtgataca taaagcagtc tcattccgtc accgtatttc
     4921 cggtgatatt ccatctctga ctaaacatct gcaatctcaa caaccaaaga caaagaaagc
     4981 tattgttgca aaggcagctg cacaaggtgc gtgggcagat gtaaaattaa ctgcaaaagc
     5041 aggaataaat actgcattag ccgctgaaac cacagcctta acaatcaaag atattggctg
     5101 gtgtgaagtc aagcgtaaaa tcgcttataa atatcaacat gacgtttcca atgtagatga
     5161 tgcaaatcac ggtcttcttg ccagcggcaa aattgtaaag gatgcagttg ttggattcca
     5221 gcgatttcag cggcaaaaag ttactctaaa gcaagaacgt caacgacttg tacagaaaaa
     5281 gcagcataca atgctgcttg cggaaaaaac aaatcaaagc ttacaaaaga gtgttcatcg
     5341 ccaaatagaa aagaaagctg aatttatcag ccggaaaaaa gatttccgca tatccaaaaa
     5401 gaatggcaca gttacgccgc ttcaaaaaac tgcattaaaa aggcgaaaac aagtctatcg
     5461 ttttgagaaa aagaagtatc agaaattcaa gcgtactact tacaggattc agaaaaaaga
     5521 aaccaaggct tatttcctgc aaaagaagat cgtaaaattc agcaagccta aaccgcttat
     5581 ttttatgccg attagctatg ctggagcccg tgccaaatct agcagctggc aaaaagctgt
     5641 cagtgcagat gctgacaatg attttatgcg attagcagat gccggtgttc gcacggcaag
     5701 acgctttcaa aaatcacccg cccaaaagac aaaaaaggca cagaaaaatc aaaaatccct
     5761 acagaaaaaa tccagtcaaa cgcaagtcaa gttaaagcag caggaaagca aattaaagca
     5821 atccagcagc aaacagcagc aaagaaaaaa gaaagcgaac cacaagaaaa gaacaaaaaa
     5881 gaaaagtagt ttaaaagaac gttttcgtcg tggattccat aagggaattg aagaagccgc
     5941 caaaaaattt atgattcctg ctttacctgt tttattcggt ggtctcatct ttctggttat
     6001 tcttgtactg atttttggtg tctttgcaaa ttccggcttt attatgggta catatgccgc
     6061 tttggacgat tctctctcca aatcagaaat ggtatacacc nnnnnnnnnn atacaccaat
     6121 tttgcaaata acatgaataa aaaggtgctt gctgttggaa atgaatctct atggaaaata
     6181 ggattaaatc aactgggtgt ttctttggaa atcacagaga attatgatga cactcctact
     6241 gaattcatct ttggacaatc cgctgtattt cctgacactc cggcatatga tttcaatccc
     6301 aatcagttgt ggagttttct ctgtgcttat tattatgact ttgacgcatc agagaaagca
     6361 aaagataacg aagaagattt tgatgtacct tactgggaat atgactccga cacttacaaa
     6421 attataaaaa agctatttga gttggagtac acttttgatt accggtacga taacgcatcc
     6481 agctggagtg aattaggtac atacacatac gattggaaat tccattatgt gactggaagt
     6541 ggaaccgaaa atggctatcc ttacatcgat ttcaaagcca ttccaaatga cttaaaagac
     6601 tttgcaaaag taaaatcaaa ccgcatttat tacaatgccg aaaatggtga aattctaaat
     6661 gccagaaaaa aatacaaagc aaccggctgg tacttacaag atcaacgcta caatgtgatt
     6721 gatccttccg gaaatgaatt gccttcattc tacacacaag atacaaacgc atttcagtat
     6781 gattactctt ataccggtgg atatggacgc ttctatacat ttgaaagatc aacctattgg
     6841 attcccagca gcaaaaaaac atctgatcct gccttttgtc ctactgttgc cccctatgac
     6901 gttgccttct tcaaattaaa taacagtaca gatgtgtcta tcaaaaatgg tttttctggc
     6961 tatttgggct ataaaagtgc tatggaaaat aagaatctaa ttacagatga ctggaatcgt
     7021 cattacgcct ataattcttg ggcaaaattg aatgcagaag ctggtggctt tttgggatat
     7081 ggtttctgta cctattatca aaaatacgag tgggtaaaaa attgcacact gtactataca
     7141 gtgcatcaaa attacacatt tgacgaagcg atcagaaaaa ttctaagcga gcaatcagat
     7201 gccgcagatc gaattgctta ttatgaactt ctttgtggct ccggtagtac tggttctact
     7261 accaatctat tgggcaatca tcaaacattt ccatcccctg tagatggtgg cattgttgat
     7321 ttaattagcg gcaattggat ttacaatggc tatggatatg atatgcacgg ttggaatcag
     7381 gaacactgct ctatcaatca gcataacgga ttagatatta ttcgcaattc cggaagcaac
     7441 gtatacgcca tggtatctgg aaaaattaca gattatgacc ctgatacgca ttctcttacc
     7501 attgtttctt ctaaaaaggt agacctgtgg tacgataaag agcgaaaagt aaaaatccta
     7561 tacagcaacg tcaaactaaa aagtggcttg aataatgggg acgttttaag tgccggagaa
     7621 aaaataggca cttctaccag cgaccgaaac tgcgttacta ccaaaaatga aaaagcagtc
     7681 tacgactatg ttcacgttca agtatacatt agctatgact ggaacagcta tgaaatggct
     7741 gannnnnnnn nnnnnnnnnn nnnnnnnntt attaaaacgg aaaggcaatt tgatatgaaa
     7801 gaaactacaa aaaggaaatt gaagcggctg catcaaaaac tgaatgagct ggaagaacgc
     7861 atgaaaaaag atgcaattga taaacgagct attcagaagc agattcaaga cattgaaagc
     7921 actgaaattt ttaatatgat tcgaaagggt ggattatcct tagaagttgt ttgtgatgat
     7981 ctggaactag gaaagaagtt gcgtgaatcc ggcataacag catcagaaat tgcagaaatg
     8041 attacatcgg aatcaaatgc agaaacaacc gctatgaaag aaacaagcac aacccaaaat
     8101 gagaaagaga ggaatttcga atgaaaagaa aatggattgc agcacttgct gcactgacaa
     8161 tggcattgcc ttttcccatg agcatttctg cttctgcggt aaatgaggat attcctgcac
     8221 aaactgatga aagcatgatg ggacaagaag aaagcagcaa taccgatgca gcacaagaat
     8281 ctgcggatca aattctggga gaaattcttg actccaacac agcaaccgga aatgcagatg
     8341 gcaactctga ttcgtctcag attgattatg aaaatgatcc ctactatgat accgaaggaa
     8401 atgcatcact gatcgagaat cagaagatta tctatgattc tgatgagatg cagtttatct
     8461 ctgtaaccac aaaaaatgga aatgtattct acatcttgat cgactatacc gctgcaaagg
     8521 aaaacggcgg tgaaaatgtt gtttattttc tgaacaaggt agatgattat gatttatata
     8581 ctctgctgaa cgatggaaat aattcagata attcggtaga ggactacgaa aaacagaacg
     8641 gcactgcatt tggcaagacc acatcttcta ttgatgaatc cgaaaatgca gtagaaacaa
     8701 ctacttccac cgctgctgct tcgtcaagtt cgaaatcagg aaacaaaaat atgatgatta
     8761 ttctttgcgt ggtagttgtt atcggtgctg gtgctgcaat tttcttcttt aagttcaaaa
     8821 agaaaaagtc taccgttcca aatgatgatc tggactttga tgagtacgat gaagatgagg
     8881 atggtgacga ataagctatg aaaaaagtta ttgtaggtgg aatgattgca gttgcagtaa
     8941 tgggtgtact ctttgctctc tccatcaagg gagaaattga tgcccagaaa catcctgtat
     9001 acatcaatga agcacgattt cagaacagcc gcaatcagaa aaaagcgtga aaggaggaat
     9061 tgctatgcag ctggtgattg cagaaaagcc cagtgtagga cgatcagtcg cttctgtaat
     9121 aggagcaact gaaaagaaag atggctattt ggaaggaaac gggtatattg ttacatggtg
     9181 cattggtcat ctggtacaac ttgcaagccc agataagtac gattccaaat acggtactaa
     9241 aatttcagaa tggagttttt caacgctccc gattcttcct gaacactgga aatttgttgt
     9301 ttcctcaaca acaaaacaac agtttcaagt aattcaggaa ctcatgcagg attctcgtgt
     9361 agatgaaatc atttgtgcta cagatgccgg acgagaaggt gaatgcattt tccgctatgt
     9421 ttatgaaaaa atcggctgca cnnnnnnnnn ncggctgcac aaaaccttgt aaacgccttt
     9481 ggatttcttc aatggaggat aaagcaatcc gtgagggatt ttcaaatctt cgtcctgaca
     9541 gtgactatga caacttatat gcagctggac tttgtcgggc aaaggcagac tggcttgttg
     9601 gattaaatag cacacgtctt tttacttgcc gttatggaac attgctttcg attgggcgag
     9661 tacaaacgcc tacgcttgct atgatcgtac aacgtgacta tgatgtaaaa catttcgtta
     9721 aacagaaatt ctttactgta gagttggact gcggcacatt tgttgcatcg tcagaacgaa
     9781 ttgacgaaga agaatcagca aaaaacattg cagaatcttg cagtggaaaa acagctacaa
     9841 tttcagaaat caaaaaagaa atcaaaactg taaatccgcc gaaactttac gatctaacca
     9901 cgttgcaacg tgaagcaaat cgtcagtacg gctatacagc acaacaagcc ttagactata
     9961 cgcagtcttt atatgaacag aagttggtta cctaccctcg aaccgatagc cagtatctaa
    10021 cggaagatat ggaacaaacc gcagctgatc tgatacaaac gatttgcaat accatatcat
    10081 gctgtcaggg attatctatt ccagaaccaa atgtaaaacg ctgcatcaat aattctaaag
    10141 tctcagatca ccatgcaatt attccaacag cagaaattgc acataaaaaa ttagcagatc
    10201 ttcctgatgg cgaaagaaat attctcaacc tgattgcagc aaaattgatt cttgcaacag
    10261 ctgatccaca tcggtatgaa gcaactaaag tttcagttat ctgtgaaaac cacaattttt
    10321 ctgcaaccgg caaagcaatt ttaaatgctg gctggaaagc ctttgaaatt gcaataaaag
    10381 aaatgctgaa aagcaatgag gacactgtaa aatctggaga tgaaaaaaca ctgccgcctc
    10441 tcgaaaaagg acaggtattt gaaaatgtaa cttcttctgt tatagagcat tataccagtc
    10501 ctccaaagcc atatacagaa gatacactgc taaaagcaat ggaaaccgca ggaaatcata
    10561 attatgatga aaatgctgat gtagagaaaa aaggacttgg tactcctgcc actcgtgctg
    10621 caattttgga aacgctggta aaacgagcat acattgagcg aaagaagaaa cagatcttcc
    10681 caactgcaaa gggcattagt ttaatcgcag tcgttccaga tgaggtaaaa tctgcacagc
    10741 tgaccgcaga ctgggaaact cagctgcaag agatagaacg tggtcaatgc aatccagatg
    10801 attttatgca tgaaatcgtc tcatttgtaa gtgacatttc tggaaaatac aacgaaaaag
    10861 ctgaaaatgc tgcttttcag gcacagcgaa cagtgattgg aaaatgccca aagtgcggaa
    10921 agaatgttgt cgaatggaaa aagtcttttt cttgtgagaa tgataaagaa aaatgcggat
    10981 ttatcatatg gaagtccatc tgcannnnnn nnnngattta aaagcaaagc cggcaacgaa
    11041 tttgatgcct atctcatctt aaaagatgac aaaaccaccg gttttgaatt tcctccacga
    11101 aagaagtaat tctatgtttg aaaaatttgt aggcacttac atttccgcac ttctcgtcac
    11161 ttcatctgtt tcaccatcta cagaaccagt gacaatacag ccacatccag acacgtccat
    11221 tcattgcgtt tctgatttgc aagtttctcc agtttccgtt tcatgcttaa aagtgacatg
    11281 gacagcagag cagaaccgtg attatacggt tacctgtacc attttagatt ctgacatcgc
    11341 atacgttgat aatatgtgct ttgtttttaa atcgaattct ctctgctata ttactggatt
    11401 acgggaaggg acttcctatt caattgatgt gattccaatt gctaaagaag gagaaacatc
    11461 ggaaatgcaa tccacttgtg catatgccaa aacggaacaa gtagaagtga ttgaaaattt
    11521 cccgtatgaa gatggttgga caaattgctt tgcatacgaa aatgctgctg gattaactag
    11581 gaatccttct tggtctgcaa tacggggatg tattcctgac ccgattacag acactggaat
    11641 tatgcgtgat gaatacggag attactgtgt tgcaatgggc acaaaatacg gatattgcaa
    11701 cgacagattt ctgatcactc tggaaaatgg gattcaattt accgttaaaa tttgcgatag
    11761 caagggggat gttccctatc atcatttttg tggtacagga aaatgtgtta tagaatttat
    11821 tcatggcaac ggacaacttc caaattgtgt tgcttttacc ggaaactacg gctgcttttc
    11881 ttggaatggt cttgactttg ataacattca atctatacaa aaaatcaact acggcaatcc
    11941 cattagctat tgatgaaagg agcgacatat gactactgtc ggcaatatcg actataaaaa
    12001 aatcgaaaat cgtcaatttg tcactgtcga tacagacaaa gccgctgaca ttatttctga
    12061 attgacgcaa aagcaagtgc ctttttcggg aagatatgat gattctcgat tgacattaca
    12121 tttttcccaa aaggactggg acattgtaca aaatgctctt tctggagctg cacgtccgca
    12181 tgaaaacgat acttcaaaag cccagcaaga attagatgac ttgcagcaac aattaaaaag
    12241 ggctttggaa cgtcaaaaag aattggaaga acaggctgca aaagtgcagc aaagtcaaga
    12301 caaggcagaa tacaccgaac caagcgtatt ttcagggcaa ccacaacctg aacaacttct
    12361 cccgatctta caagcaaaag caatccaaca cactgaaaaa ttagacgcac tttccgaaaa
    12421 aaggactgca ttagagaata aaattcaaac caatcaaggc aaaatcgata gattttcaga
    12481 taaggtacaa cgacttattg ataccaatca aatgctccac agtttatttc aaaatgcgaa
    12541 agttcccggc atcaaaacta tggcagaagc tgcgattcat cgcaacgaac aaaagattat
    12601 aaaaattcaa gaaaaacgaa ttccgaaact acaaaccaaa atacaagagc aatctaaaaa
    12661 gattgagaag atcgacagga aaagcgacat tacccaatgt aaacttgaca gatgccgttg
    12721 tattaaccaa attatcaaga gtttcggtat ctctgacaat gctgatagac gacaagcctt
    12781 ttcgcaagca atggatgggc tacatcaaac cacaatacag cgattatcct ataaattaga
    12841 ttcttgtcag cagaagatct ctctactgtc tcaaaaatac atggcaacag acagtgcggc
    12901 tgataaattg aaaatccatc aaaaaatcaa tactctaaaa gatcgaaaac aaaaaatcac
    12961 tgcaaagctg gacaaacttc acagtgtgac attaccttat gccaaacaat ccagtactac
    13021 cattgaccat gttatttcag gaactgaaca gcaattaaaa gaagctgcgg agactggtaa
    13081 aataaacatg cccgtatttg cagaaaaaat atgcgtagat aatgctgatc ttctgcctga
    13141 aatagctctt tctgtaccca tagaaaagcc cgatcagtct cttatccctg aaattgcaga
    13201 tcttctaaat atgtctgtgt ctgaagtgga aagcaaacca ttggatattc aggcaatgtt
    13261 aaaagacaca tatactgcta attacttttc cgatccctct actttacaag aaacgcttac
    13321 ttacattatc aatcccaatt atgaaacaaa ccgtgatttg caagagcatc aagaaaaaga
    13381 taaaatgtct cctgctgttg ctgccaaaga agcggattct gattacagaa agcatgaaca
    13441 ttcacaggaa atgcctctcg gaaaaacagc tgcaactgca agtagtgttg ctcaaagcca
    13501 ccttcgttct gtagaagaat tgacagaaac caatgcaaat atgatcgatg gaatcctcaa
    13561 caatgaaaaa ccagaaccag agcctgaaaa acctcaaaaa gcagaatttt ctttttccag
    13621 aaacagcatg aaagctttag cagcaaaggt tcatgatatt ccgtccaatc cccctgaaaa
    13681 gcagagagat gaactgaaaa agtaatggaa ttataagaga aaaggagaaa cgatgaaata
    13741 ctacggcaat caaatctgga atgcagcaac gccaagtatt cgaaaagcta cgcttttaat
    13801 ggacgaagct gcctttcgta ttttacaaca gcagctagaa caatatcgat taaactatta
    13861 tgcagccgag aaaaacggca aagtgaaaat ggtcattaac tataatgatt ctcacgatat
    13921 tcaaaaatta attggcaaaa aaacagcttc tcaaattcat tggtttccaa aatcaagaca
    13981 ttatgcacca ccggctttta acattattgg aaacaccaag tatcaagaat tatctcaaaa
    14041 gagttatatt cgaggtaaca cagaccttgt tcttcaagtt gccaagcaat tagaagaaca
    14101 aggtatttct ttttcaggga gagtctacaa tcaaaataga gctaccctca ctgtacagga
    14161 tcaggatcaa gaaaaggttc ttcgtttcta tcatgatact tcagaaaaaa gagctgcaca
    14221 gcatcaagct gctgctgcat tagaaactgt tgatatctcc cttcccaatt taccgcagca
    14281 aacatttgac gaaatcctcc cctatctaaa acaaacagat atttcattct cagcacgtgc
    14341 ggaaaaagat cattgcattt tttccatttc tcaaaaagat accggtatct tctatcaagc
    14401 tttagtacaa gcaaaatttc gttctgaatt tcataaaagt ttaaaagaac ttggatttac
    14461 agaacaacaa gatgcatttt tagagccctt ttctgttttt cttgccacac aggaagcatc
    14521 aggaaaatta acattagatt atacagcata tctcaatccc aattacagca atcaacaatt
    14581 acaaatggtt tgtgaccttt ttcaagagta tcacaaacaa tccattacag aacaaatttc
    14641 tgcggacaat ccagtattgc agaaattaca acaacagaaa tctcaatttg acgcagaaat
    14701 taccctaaac agcatcattg ggaacaatcc ctttcagcca gaacaaaaga atgctctaat
    14761 cgctgccatt caaatcggca ttcctcatga attattgaaa aaaatcgata acacttattc
    14821 agcagaaacg atcaatgact tcatacaagt atatcagacc tatgatatgc aaaaaataca
    14881 atcttttttg gattcacatc agcctgaaac tgacaatcac tcacagggnn nnnnnnnnaa
    14941 gaatcatatt acaaataaca taatttcttc tgcttttgaa aaatcgcatg gtttccgttc
    15001 tgcatacggc aatgaaaaat tactaagcag tcgtatcttt ttcgggagat tgcatccagt
    15061 ttcagaagca attgaacatg ctgtttccaa taaaacaact gtgattcaga ccgaaccaca
    15121 aaaagcagaa ccaaaaccta ctgctgaaaa agaaccatcg tctgtcacac aggaagactt
    15181 cgatgtactt cgcacactag aacctcgaaa aagtattttg aatcttaccg aggacgaact
    15241 tactgcaatt ccaacatgga aagagcgatt tcaaaaagaa ttggctgaaa aatccccttt
    15301 ttatcgattg gaacaaggag aatggagaga tcaggaagca acacaaatac caattctttc
    15361 aattcctagc cacgatgcag attttcaaag tgtccgtgcc gatataaaaa gcagaaatat
    15421 cgaacgaggt tcttttacca atacagatac cggctggaca attcaagtta gccgtaatgg
    15481 attagaagat tccgtaaaat acggacacca acatcatgat aatgctgtgt atgatatgct
    15541 ttaccaactt cccaaacttg ttgaagaaag cattttcctt gacagcacta tttcagaaaa
    15601 aaacaataaa aataaagcaa acaacacagc ctttatgcac aagctatacg ctgtttgcaa
    15661 aatagatgat cagccctatc ttgccaaatt aaccatagaa gaatttgcag atgggcaaaa
    15721 agatacattg aaaagaatgt ataatgttca ggatataaaa atagagccac ttaggctcct
    15781 agagttcact gataaacagc tagctcgttc agtcctaaat gactccacta ttagtatagc
    15841 agatttattt actgttgtca aggaatttga ccacgatttc tacttaaacc gtactccgca
    15901 aaaggcttcg gaacatacaa ttcaagccga gcctaatatt cctgttccag atgaaattac
    15961 accggattcc tctatagaag aaagcacgcc tccgcctata gaaaaaaaca cagattccat
    16021 tgaggaggat acagctagcc attctcaaaa atcagaagta gcttcatcgc ctgaaaaaga
    16081 gaaaaatttc actatcacta atgaatcact tggcgaaggc ggtttaaaat caaaattcaa
    16141 gaacaatatc actgccgtga aaaccttaaa aactctggag caagaaaatc gttctgcgac
    16201 agatgcagaa aaagaaatcc tttcaaaata cgtcggttgg ggtggaattc cgcaagcttt
    16261 tgataagaat aatgaatctt ggagtacaga gtatacacag ctaaaggaat tacttacgca
    16321 tcaggaatac agacaagcga atgcttctgt tttggacgca ttttatactt ctcctgtggt
    16381 aattgatggc atctatgaag cacttgaaaa tttcggtttt caaggcggaa atgtattgga
    16441 accagcaatg ggtgtcggaa acttcttcgg acgtatgccg gaagaaatgc aaaaaaattc
    16501 tcggctttat ggtgtcgaga tcgacagcat atccggcaga atcgcccaga aactctaccc
    16561 cgatgctgac attgcaatac aaggatttga aaaaaacaat tttcaaaatg gatgctttga
    16621 tgttgctgta ggaaatgtcc cctttggaga gttgagtttc aaagatgaca aacatggtac
    16681 aacaaaactg cacgactatt tcttttccga aacactggac aaagtaaaag acggcggtat
    16741 tgtggcattt gtaacatcag ctggaacgct ggataaacgt gatgaatcca ccagaaaggc
    16801 attagcggaa aaagcagact ttattggagc aattcgctta ccgggtggta aaaatggggc
    16861 atttaaaagc aatgctggca cagaagtcac aaccgatatt atctttttgc agaaaaacga
    16921 gcataaagtt cccgatccag aaaaagaaac ttggatttct attggcgaaa cggatgaaag
    16981 tttgccaatc aatcgatatt ttgccgaaaa tcctaacatg gtgcttggga ctgttgtaga
    17041 aggaaataag ctatatggca gcggaaccat ggttatagct gaagatggtg ttgatctaaa
    17101 agaacagatc tcacaggctg tatcacaatt gtcagctaca atttcggata caagaacaaa
    17161 agaagtatat gcaaaatcag cagacagtac tgctatcgaa attccatctg ttcttcgaaa
    17221 ttatagcttt tttgcagatg acaaaagcgg agaaatctac tacaaaaagc ctgatcaggc
    17281 gattcgctgg aatggaaatg attctccttc caaaaagaaa cgtatggaag cctttctcac
    17341 gcttcgagat gttacaagag gaattttgca ggcacaagaa gaaaattgct ctgacacggt
    17401 tctaacggca ttacagggca aattaaatca gtcttatgac acgttctatg aaagctttgg
    17461 cttacttcac agttcctata acacatcaca actttcagat gatgtttcct ttcccttagt
    17521 ttgtgcacta gagagcaaat ttgaaaaaga aaagcttatt gcaaaatcag acctctttac
    17581 aaaacgcacc attcagccgc ccaaaccaat tgaacacgtt gatacaccgc aagaagctct
    17641 tgcattatca atggctgaaa aagcgtgtgt tgactttgat tatatgcaaa agctgactga
    17701 cattcccaag gaagatattg tatcagcact gacgttagca aaaagcattt atcctgttcc
    17761 agaatgctct gacgatgaga agatcatcta tcaagaagca tctgaatatt tatctggtga
    17821 tattcgaaaa aagttagata atgcaataga agccgcaaaa agcaatccac tctttgaagc
    17881 aaacatttct gcattgcaag aagtaatgcc tgagccccta aaagcaggcg atattgatgt
    17941 aaaaatcggt gctacatggg tggatcccaa atattatcag caatttctgt acgaaacact
    18001 gcatacgccg caatcgcttc aggcatctga gagagattcg tggatgagaa aactatttgg
    18061 aaagaaatcc attgcaatag aatttgccga aaatcaatgg cacatttcta ataaagggtc
    18121 tgaccgttcc gtaagtgcta cacaaactta cggtacatcc agtaaaaatg cttatgaaat
    18181 catggagaac ctcttaaatc tccgtgagac aaaagtctac aagtcggtgg gatacggaga
    18241 agaaaagaaa agcgttttag atttagatgc cacaagagca gcacaacgaa aagcacaaaa
    18301 aatagaagaa tcctttgaat catggatttt tcaagatcca caacgacgtg cagatctagt
    18361 agacacctac aatcgcaaat ttaatgcaat acgaccgaga gaatatgacg gttctcactt
    18421 aacatttccg ggcatgaatg ctggcattca gttacacgga catcaaaaaa atgcaattgc
    18481 ccatgcaatt tacggcggaa atacactttt tgcacacagt gtcggtgctg gaaaaacctt
    18541 tgaaatgatt gcaacggcaa tggaatccaa acgtctaggc ttatgtaaca aatcactttt
    18601 tgtagttcca aatcatttaa caggacaaat cggcgacgac ttcctaaagc tttatcccaa
    18661 tgccaatatt ctcgtgacca ccaaaaaaga ttttgagaaa aagaatcgac aagaactttt
    18721 tgcaagaatt gcctctggaa attttgatgc agttataata ggacactctc aactgaaaca
    18781 tattcccctt tctcaagaac gccaagaaag acaacttgaa gaacaaattc aggatattct
    18841 ctccagcata gaacaactga aacatgaaaa tggtgatagt tttcaagtaa aagctatgga
    18901 acgcaccaga aaatcattgc aaaaacaaat tgaaacgcta aaagtaaaaa aacaagatga
    18961 tgttattcca tttgaacagc tgggcgtaga ccgcattttc gtcgatgaag cccacgaatt
    19021 taaaaattta ttctgtgcga caaaacttca aaatgttgct ggtatatcaa attctgcatc
    19081 tcaaaaagca ttagatcttt ttcaaaagtg tcggtacatg gatgaaaaaa caggcggcag
    19141 aggcattgtt tttgcaactg gcacacctgt atcaaactct gtcactgaac tacatacaat
    19201 gatgcgttat ctccagtatg attttttgca ggaaaaagga ctacatcatt ttgatagctg
    19261 ggtttcgact ttcggcaagc aaaaaaccga ttgggaactt gctccggcag gaaataaatt
    19321 caagcagcgt accagaatcg caagctatgc aaatatgcct gaacttacta gtatgtttaa
    19381 gcagtgtgct gatgtaaaaa cagcggatat gctgcactta aatgttccag agtgtgaaat
    19441 gcacattgtc aatgtagagc ctacagaact tcagcaaaaa cttgttacag agttggctga
    19501 cagagctgat gatatacaag gtggagctgt ggatccggaa gtagataata tgcttcgcat
    19561 taccggcgat ggaagaaaag ttggtttaga cccccgtttg attaatcctg attttgagga
    19621 taatcctcaa acaaaattaa atcagtgcat agataatgta taccgtattc accaagaaac
    19681 cgcatcagac aagcttaccc aaatcatttt ttgtgattta ggtgttccaa gaggaaagcc
    19741 ggcaaaaaca gcaaaatccc aatctgatga agaagaaatt atatctgatt ccttagaaga
    19801 aagcggtgat ttctgcatct atgatgnnnn nnnnnncatt aagaaaaagc ttatggaaag
    19861 aggaattcca gaaaaggaaa ttgcttttat tcacgatgca ccaacggaaa aagcaaaatc
    19921 agaacttttt gaaaaggttc gatgcggcga tgtacgagtg ttgattggta gtaccgccaa
    19981 aatgggaact ggtacaaatg ttcaagatcg ccttgttgca cttcatgatc ttgatatacc
    20041 atggcgacca gctgatctgg agcaacgtcg tggaagaatg gttcggcaag gaaatatcaa
    20101 caaacaagta cacctatacc gctatgttac caagggaaca ttcgatgcct atagctatca
    20161 gacgttggag gcaaaacaac gatttatcag ccagattatc acttctaaaa caccagctcg
    20221 tacttgcgaa gatgttgacc aacaagcatt gtcctacagt gaaatcaagg ctctatgcac
    20281 tggtgacgaa cggatcaagg aacttatggt gcttgaatcc gaagtcaaag aattaaagtc
    20341 catgcagtct gagtatcaaa atgtacacta tgatatgcaa gataaacttc aacgctaccc
    20401 tcaaacaaag cagcagcttg aatctagtat agatgctatg aaaaaagatt tggagcattg
    20461 caaaagtctt ccgattgatg aaaatacaca tctgccagca ttcaaaataa cgctagacgg
    20521 aacaacgtat acagatcgca ccgaagctgg aaaagcatta gaaagcgtgt gctgcaagct
    20581 gatcggcgaa tctaacaaac ctttgccaat tggcgaatta catggttttc caatcacaat
    20641 acgatttgca ccagatcaga attgcctaca ggcaacattg caaggttcaa agccatacac
    20701 aacaaacttt tccgtttctt tcccaacaaa tctaaagcat ctggaaagcc tgttattttc
    20761 attagatcaa aaaattgcag cagctcaaaa cgctctaaac caattgaatt tagatgccaa
    20821 agaagctgaa aaaattcttg ctgaaccgtt tgcatctgga gatattcttc gagaaaagtc
    20881 ggaacgttta accacacttc gagatgattt gaatcaagaa gcaatggaag ctgcaaaaag
    20941 cggaaataaa ccacaacgaa cattttattt tgaaatggca aaattaaaga aaaatgcaag
    21001 aacaatttca aaatccactt tggaaaaagc agaaggaaag aaaaaggcgg aatttctgga
    21061 ataataatct cagcattgct cgatcgattt tttcaaaaag ttcttgacaa acggcatgat
    21121 ttcgggtata ataataatga agataagctt tgacttatca tatgaaggca tcacgccttt
    21181 gtaaaaagca gtgagtccta ctgtgagtgg aaccgataag agcggtgagt cctaccgtaa
    21241 gtggaagcga ttaaaaaggc tgcggatacg tccacagcct tttttattta tatcagaaaa
    21301 gtcttgacaa atgctaaggc tttgagtata ataaaaacgg agataagttt cgacttattg
    21361 tatgaaggtt ctttgccttt gtaaaagtag tggaccctac tatgagtggg agttttaaaa
    21421 gcggtggacc ctactgcaag tgggagtttt aaaagcagtg gaccctactg tgagtgggag
    21481 ttttaaaaat caaagctgcg ataatattct tatcgcagct ttttcattag aaataaaaag
    21541 gagaaaaaat ggaacaaaaa cgactacggc tatacaaatt agatatgaaa tacatccgtg
    21601 atctcgctaa aaaagatgat aatgttcatt ccgtttcacc acaagtcgga aaagataatc
    21661 gtccatttgt gggaattatc attgtatgcg acaataagaa atactgcata cctctgagtt
    21721 caccaaagcc aaaacatcaa aaaatgaaaa atgacataga ttttatgaaa ataaatgttg
    21781 atggaaaatt actaggcgtc cttaacctca ataacatgat tccagttgat gaaagatttc
    21841 ttaagcctgt tgagctacgc attacccgac aagattctcc agaaagtacc cattataaaa
    21901 aaatggcagt aaaacagctg aactggtgtc agcaaaatca agatttaatt gttgcaaaag
    21961 caaataaact gtatcatcta gttaccgata cgccggataa aatgcgtatg gtcactaaac
    22021 gttgctgtga tttcaaaaag cttgaagctg ttctggagaa gaaattgctc tctcatcaag
    22081 agcaacatga acagccttca aagccgtctt cttttacatt ttctagcaag cagagaaaac
    22141 aagttcagtc tgctgtgcag cagaaagaat cagataaacc tcacactcag gataagaaaa
    22201 gaaaaccatc actatgacgt taaaagttca attcaaagcc gagccataaa aaacaattcc
    22261 aatcaactca ctcaaggata tactaactta aaaatttaat ctataaacaa atatggagta
    22321 cattcttcaa ttaattagaa gaccatgtac tccattttta tttgagaaag tattatcaat
    22381 tacgcttaat agaattatct atttcgtatt tttttcacaa acaccgatat ccaatatcgc
    22441 ttgtttagct aatgtgtata aagtgctgtt tttaaaattg atggtttaaa caattttgca
    22501 tatactcatt taattcaaat catctaagaa ttacagtgaa tcataattcc tattgtcatt
    22561 gtgttatcac gttttccttg ttgcttattg gattaccctc agtttttgat gcagtaatat
    22621 gtttttgatt ttcaccaaaa aatgcacatt gccaatccat gtaattacaa agattttccc
    22681 aagtaacatt tgcttcggaa agggcggcag gatacaagta aattgtcatt ttctttgata
    22741 caaactcgtc ctgagattta gaggcaaaag aaaaaagtgt ttcgtgtaca acatcttcaa
    22801 gagttccatc ttttagcccc gctctaccag tcccaattac tggtattgca ataggttctg
    22861 tatgtccttc tcttgcaaga aaatcccaaa gtccaaccaa agccttcgtc acattttcca
    22921 tagttacatt ttgtggctta cctgttttgc ttacatccgc tacggcaaga aaatagaaat
    22981 gctgtccttt tattgtaact tttgccactg ttccaatatc gtatcgatta caattcgttt
    23041 tttttctatc acttaatttt tcgtattgac tgggaaaata ctcgtcaaga gatcttttga
    23101 ttgctgcatc taactcagaa aaatctgtac cataatgctt tctttgaaat tgaccttgta
    23161 tgctttttat acttataaag tctccgctca ttgtagtgtc aaatgtggtg ttggtaggaa
    23221 taactactgc agaatccttc aatgtaagta tgtcgcctat gcaaaaagac aggagcgtat
    23281 cttttgatcc caagtaagca gaatgagata acggctcctt atgtaaaagg attgcaatga
    23341 gcacaacgac caatagaaat gactttaaat gatttctaaa aaaaatcatc aatatggtac
//
LOCUS       DAWBGR010000015        23048 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_813_length_23048_cov_4.826947, whole genome shotgun sequence.
ACCESSION   DAWBGR010000015 DAWBGR010000000
VERSION     DAWBGR010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 23048)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23048)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23048
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_813_length_23048_cov_4.826947"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9283..9292
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5733 a         5779 c         6369 g         5157 t
ORIGIN      
        1 tttgcaagca gatggcgtgc aaagtcgcca gacgctcttt gtgcggtgtt gcctttgaag
       61 aaaggaatgg gtttggactg tggagcagga agcgtttctg acatggaatc cttggcacgg
      121 ctgccggaaa tacagcgaag gctgtgcaca ttgctatatg tattatctgg atgcacagcg
      181 tgacagggac ggcagcgaga tctatcggac aaagaccaat ttcaacctgc ccgtcaaaaa
      241 gactcgacag ggtgcataca agattccgtc agggacaatg ctgcacgtct gtatgaccag
      301 cgactttttt ctgcaagagg cagacgactg gcgggaagaa gtatgggaca tgatacgctg
      361 ccgcagagat gtgcacttct ggattcagac gaagcgggca gaacgtatgg cagaccacct
      421 gccggaagac tggggcgacg gctgggaaaa cgtgacattg tgtgtgacaa cggaaaatca
      481 gaaatgtgca gaggaacggc tgcccttatt gctggcagtt ccggcaaagc acaaggcgtt
      541 catgatcgca ccgattcttt ctgaagtgca tgccgaaaag taccttgcca ccggacaggt
      601 ggaacaggta ctctgcgacg gggaaaacta cgacggcaac cgcccctgcc gctatgagtg
      661 gatcagatcc ctgcacgacc agtgccggaa cgcagacgtt ccgtttcggt ttctcggaac
      721 cggaaactat ttctgtaagg acggaaaggt gtactgcatt cctaaagcat atcagcgtgt
      781 gcaggcacag cgttccggtt tgtcctatcc ggcaggagat acccacgtac ccatgcagcc
      841 acgttgccag aactgcaaaa gaaagcacag ctgcaacggc tgccgctggt gcggaaagtg
      901 tcattgaatg ctctcttgca ctgagaacct gtcaggcaat accgaagaac acttgatgaa
      961 tgaggacaaa attatgaatg tacgagcaaa aatagagccg ctgctgttac aggcacagaa
     1021 gccggcacag tatatcggcg gcgaggtggg cagtatctgc aaagagcggg aatcagtcga
     1081 tgttcggttt gcgttctgct ttccggatac ttacgaaatc ggcatgtccc atctgggcat
     1141 gaaaattctg tacagtttaa ttaacgcacg ggaaaacttc tggtgtgaac gggtctttgc
     1201 accctgtgca gactttgaag cactgatgcg ggaataccag attccgctgt atgcactgga
     1261 aagtctggat ccggtgcggg aatttgactt cgtgggcttt acgctgcaat atgagttgtc
     1321 atataccaat atcctgaata tgctggactt ggcaggaatt ccgctgcatg cctgtgatcg
     1381 cggcgaagca ctggcaccgc tggtggttgc cggcggtccc tgtgtctgta atccggaacc
     1441 gctggcggac tttttcgatt tgtttattct gggggagggc gaggaagtca atctggaact
     1501 catggacctc tacttaagaa tgaagcggca gggagcaggc cgctctgcat ttctccgtgc
     1561 ggcggcacag atcgagggga tctatgtgcc ctctctgtac gaggtgacct atcaggcgga
     1621 cggtaccatt gccgcagtga cggcgaaaga cggtgcaccg gaaaaggtga ccaaacggat
     1681 cgtacaggac atggacaagg cgttctatcc ggaaacattt gtggtaccct ttatggaaac
     1741 ggtgcacgac cgtgccgtgg aagaagtgct tcgcggttgt atacggggct gccgtttctg
     1801 tcaggcaggc ttcctgtacc gtccgtttcg ggagaaatcg ccggaaacca tcgtccggca
     1861 gtgccgttcg ctctgcgata ccaccggcta tcaggaactg tccctgtcct ctctgagtac
     1921 cagcgaccac agccagctgg aagaaacgct gaacgaactg ctgccctatc tggacgaaaa
     1981 ccgcatcaac atcaccctgc cctctctgcg ggtggacaac ttttccaagg aactgatgga
     2041 tcggattcag ggcgtgcgga aaagcagtct gactttcgca ccggaagcag gtacccagcg
     2101 gctgcgggac gttatcaaca aaaatgtcag cgaagaagaa ctgatgcgga ctgccggcat
     2161 tgccttttcc ggcggattcg ctacggtaaa gctgtacttc atgatgggac tgcccacaga
     2221 aactatggag gacgtagagg gtattgtgca tctggcggaa aagctgatcg acctctatta
     2281 taatacgccg gaacgttcca aaggcaagac catacagatc tccatcagct gtgccacctt
     2341 tgtgccgaag ccgttcacac cgttcgagtt tgaaccgcag gtttcccgcg aggaactgct
     2401 ggcacggcag gagcaccttt taagttgtat ccagccgcgg catcggaaga agatcaaagt
     2461 cagctggcac acctacgaga tcagcctgct ggaggcggtg cttgcccgcg gcgaccggcg
     2521 gctggtacca gtcattgaac gggcatggca gctgggcagc aaatttgaca gctggagcga
     2581 gtgctttcat tatgagatct gggagcaggc aatggcagac tgcggcgtat ccgcttcctt
     2641 ttacgccaac cgcaccagag cctatgacga gatcttcccg tgggatcatc tggattatct
     2701 ggtggagaaa tccttttttg tgcgggaaaa ccagacagca catgcttcag aaaccacacc
     2761 ccactgccga gccaaatgtg ccggctgcgg cgtaaaccgg agaatcggga gggcttgctt
     2821 ttgaaaaaca tcgttcgaat gcgttttgcc aaaaccggca gagccagata tatttcccac
     2881 ttggatctga tccgctgctt tcagcgagcc gtctgtcggg caaagttgcc ggcggcgtat
     2941 tccgagggct ttcacccgca catgcagact tcctttgcca ccacgctgcc gctgggattt
     3001 ttcagcaccg cggaacaaat ggacttggaa ctgacagagg agctgccctg tgatgcggtc
     3061 atggaacgac taaacgccgt cctgccggag ggaatccgga ttctggaggc gtggaaaggc
     3121 atgctggcgt tcaaaaagtt ggcatacgcc aattacactg tgcagattcc cagtgaaagc
     3181 agcggggaac tgtacgattc tttctgtgtg tttgcggcac agccggagat cctcacggaa
     3241 aagcgtacca aaaaaggcgg caccaaagaa gtagacctga agcctatgct ggaggtgacc
     3301 ggtgccgagt cgttgcctga tgcactggaa ctgcggctct gcctgccggc agggagcact
     3361 gccaacctga accccatgct gctgctggaa acgtggaaaa aacagacgga acagacgtgg
     3421 gcttctccgg agatctgccg gacggaactg ctcacagaag aaaaacaaaa atttttttga
     3481 aaaaacactt gctttttctg cggaaatgtg gtatgatata aaagcatttg aaatttgtgc
     3541 cgaattcggc tgaaaaagcc gcggcacaga tgatccgcac ggtaacgtgt gaggttaatg
     3601 ccggctgtgc gtccgatggg aatcggcggc tcagcagaac gacattaaat cggacagtcc
     3661 gctgacgatg ctgtgagaaa atacggcagc gttccaccgc ccaatgctga acggcggcag
     3721 aggaacacgt gaatgaatgt tcgcaaattt taggaggatt taaccatgga tgcattgaaa
     3781 acaatcagcg acgcaagcct gaagcaggaa gtaccggcat tcgcagtcgg cgataccgta
     3841 aaggtacacg taagaatcaa ggaaggcgac aagagccgta ttcaggtatt cgaaggcacc
     3901 gttatcgcaa agaagcacgg cggcatcagc gaaaccttta ccgttcgccg cgtagctcac
     3961 ggctgcggta tcgaacgtgt attcccgctg cactctccgg tcgttgaccg cgtagaggtt
     4021 atccgtaagg gtaaggttcg cagagctaag ctgtactacc tgcgtgacag agttggtaag
     4081 gctgctaagg ttaaggaaca gattcgataa tcaaaagaat catgcacaaa atgcaaagac
     4141 gggcagaatc ggtgtgcgtg tcgtcgtttg cttcatgaca cctgcctgtc ggcagtaaaa
     4201 aagggacagt attgtccttt ttttattatc ggaagactgc aaccggctgg cgaaagcacc
     4261 agcggttaga aatggagaac ggcggaatgg aacaacagac aatacaagag gaagaaaagc
     4321 agcagcagga gccgcagaaa agttcttttc tgagcgatgt ggcagagata ctggaaaccg
     4381 tattcgtttc gattttcgta gtcattctgc tgtttgccta tgtagtgcgt ccggtcacag
     4441 tagacgggga ttccatgcgg gaaaccctgc acgacaagga tcgtctgctg atgtctgacc
     4501 tgctgtatac gccggaatac ggagatattg tcattgtcaa caatacacat tcctatttgt
     4561 atgatgaaga cggcaatctg gtagagggca acggtctgcc gcaggaaaag cgactgatca
     4621 agcggatcat tgccaccggc ggacaggaga tcaacattga ctttgccacc ggcgaagtga
     4681 cagtagacgg tgaggtactg gacgaaccct acattcggga actgacagcc aatcaggaag
     4741 acggcttcgc cagctatccg gtcacagtac cggaaggcta ctatttcgtc atgggtgaca
     4801 accggaacaa ttctactgac agtcgttcgc agttcgttgg cttcgtgccg cgggatgcga
     4861 tccttggcaa ggcgatcttc cggatctcac cggacgattt cggcagtatt tataagaata
     4921 tgaagtaagt gcaataggaa aacactgcaa gcagattgcg tgcagtgttt ccatcgggaa
     4981 aggcggctgg catggcagaa gaactgatac ggaatatcca atggtttccg ggacatatga
     5041 caaaaacacg gcgtatgatc gcggcgaatt tgtcgctggt agatgctgtg gtggaaattc
     5101 tggacgcacg tgttccgcag agcagccgca atccggaaat ggacagcatg gtgggcagca
     5161 agccccgcct gctgctgctg aacaaggcag atatggcaga cccgtcagca acttcccgct
     5221 ggatcgcagc ctaccggaaa cagggctttg tggctcttgg catggactgc cgcagcggaa
     5281 agggcgtttc caattttgca ccggcagtgc gggaacagct tctgcctgca ctgctggaaa
     5341 agcgggcggc tcacggcatg actggtgcac ccattcgctt aatggtggtg ggaatcccga
     5401 atgtgggcaa atcctccttt atcaaccgca tggcacagtc caagcgggcg aaggtggaag
     5461 accgtcccgg cgtgacccgt accaaacagt gggtgaaaat cggcagcaac atggaactgc
     5521 tggatatgcc gggcgtgctc tggccgaaat tcgatgacca gactgtggca cagaagctgg
     5581 cgtttaccgg tgcgatcaat gacgatgtca tggatctgga agcactggca tgcctgctgc
     5641 tgcagcggtt agcggtgtct tatcccaagg cactggaaga acgctaccgc atgaccgacc
     5701 tgcaggcaat acaggaagat ggttttgcca tgctggaact gctgggacgg aaacggggca
     5761 tgctgatctc cggcggcgag gtaaacaccg agcgtgctgc gatcacactg ctggacgagt
     5821 tccgcagcgg caagctgggc agaatcacgc tggagctgcc ggaacaggag gcactgtaat
     5881 ggcaaaggct gcaccgaaaa agtccaccga ggaactgtac gcgtgggacg cggaaaaacg
     5941 gggcagtggc ttgctgctct gcggtgtcga tgaagctggc agaggtcctc tcgcaggaga
     6001 tgtctatgcg gcggcggtaa tactggatcc ttccaagccg ctgatcgcag ggctgaatga
     6061 cagcaagaag ataacagaaa aaaagcggga atctctcttt gcagaggtgc agtccgcttg
     6121 tctggcgtac tgcgtggcat ctgcaactgt ggcggagatc gaggaactga acattctcgg
     6181 tgccaccatg ctggcaatgc agcgggcagt ggctggactt tcggtgcagc cgcagctggt
     6241 actggtggac ggcaatcgtc tgccgcagct ggcaatgccg gcggaaaccg tagtcaaagg
     6301 agatgccacg tcggcgtcca ttgcggcggc ttcgattctg gcaaaggtgt ccagagatcg
     6361 gacgctgtat gcattagatg aacaatatcc gcagtatggg tttgcgaagc ataagggcta
     6421 tggcaccaaa gcacactgtg aggcaatctt acagtatgga ccatgtccgg cacatcggcc
     6481 cagttttttg cggaagctat atcagaaaca tgaagataaa aacaactgag attggccgtc
     6541 tgggggaaac cgcagtttgc cggcagctgg aacagcaggg ctattccatt gtccggcgga
     6601 atttctgcgt ccgcggcggc gagatcgaca tcattgcgga aaacggggag tatctggcgt
     6661 ttgtggaggt aaagacccgc aagccgaaca gcctgacttc cggattcgat gatgttacag
     6721 caaaaaaaca ggctcgtttg atccagacag cagccgtgtg gtgtgcagaa caccccacag
     6781 agttgcagcc gcggtttgat attgcctgcg tggtgatgca gggaaagcag atcctgtcgg
     6841 tggattatct ggaaaacgca tttgatacga cgggcagtga ttttatattt taggcaggta
     6901 ttctgccggg aaaagggtgg taacagtgtt ttacaagagc accagaaaca gcagcattcg
     6961 agtagagagt gccgctgcaa tcgcaaaggg gatttcggaa gaaggcggtc tgtttgtacc
     7021 ggaaaccatt ccgtcgatct ctatggacga actgaaatcg ctggctggca tgaattatgc
     7081 acagcgggca gcatttgtgt tttccaagta tctgacagat tacaccgatg ccgaactgcg
     7141 ttactgcaca gagagtgctt acacaaccaa ggcatttgac accgagaaca ttgcagagat
     7201 cgcacacctg ttcgagggaa cctacatgct ggaactgtgg catggtccga cttgtgcatt
     7261 caaggatatg gctctgcaga tcctgccgta cttcctgaca actgctgtga agaagctgca
     7321 catggacaag aaggtagtca ttctggtagc aacttccggt gataccggta aggctgcact
     7381 ggagggcttc aaggacgttc cgggcaccga gatcatggtc ttctatccgg tagaaggtgt
     7441 cagcgacatg cagaagcggc agatggtgac acaggaaggc gagaacgtta cagtttgtgc
     7501 cgtaaagggc aacttcgacg actgccagag tggtgtcaag aagatcttca ccgaccacaa
     7561 ggtgctggaa gcactggaac agggcggtat gacattctcc agtgcgaact ccattaactg
     7621 gggcagactg gtaccgcaga tcgtgtacta tgtttcctct tatgtttctc tggcagaatc
     7681 cggacagatc gcatacggtg acctgctgaa cgttgtagtg ccgaccggta atttcggtaa
     7741 cattctggca gcttactatg ccaagatgat gggcgtgccc ctcggcaagc tgatctgtgc
     7801 gtccaatatc aataaggttc tgacagactt tatccgcacc ggcgtataca accggaaccg
     7861 tcagttctat cccaccgcat cgccgtctat ggacatcctg atttccagca atctggagcg
     7921 tctgctgtac ctgctgaccg gtgaagatga tgcccagatt cgggaatggt tcactgctct
     7981 ggcggaacag ggtacctatg aggtaactga tgcggtaaag gcaaagctga cagaacagtt
     8041 ctacggcggc ttctgtgacg atgccgaaac caaggcaacg attgcagaac tgtacaagaa
     8101 gtacggctat acctgcgata cccacaccgc agttgctgtc aaggtatacg aggactaccg
     8161 caaggaaacc ggcgatacca ccaagactct gatcgcatct actgcaagcc cgttcaagtt
     8221 cagtgcaagc gttctggacg cactgggcaa gaagccggca gacgatgttg atgaatatga
     8281 tatggtagag ctgctgcatg aggcttcgaa aatggagatc ccgcagtcgc tggctgcact
     8341 gaagaccaag ccccgccgtt ttgagggcag cattgataaa tcggaaatgc agcagttcgt
     8401 attgcaggaa ctgcacctgt cctgaacggg ggttgcatgc ggttatttac cataggtgac
     8461 ctgcacctct cttttggtgt accggacaag cccatggaca ttttcggcgg ctggaaggat
     8521 taccagaagc tgctggaaca aaactggctg gaacggatcg caccggagga tactgttgtg
     8581 ctggcaggag atttctcatg gggcatgaca ctggcacagg cggaggcaga cttcgcattt
     8641 gtgcagaatc tgccggggca gaagattctg ctgaaaggca accacgacta ctggtgggct
     8701 tcgaaaaaga aaatggacga ctttttcacg gcacacggat tcaatacgct gcatatcctg
     8761 cacaacaacc actatgcctt cggacagtac gggatctgcg gcacccgcgg ctgggtgagc
     8821 atgcagggag aagctgccga tgccaaaatt ctggcaagag aagtgcagcg gcttcaggtt
     8881 tccattcagt cggctgtgga cgcaggactg gaacccattg tgttcctgca ctatccgccg
     8941 atctacggaa cgtcgatgaa ctacgagatt ctggaagtgc tgcacaagta tcagatccgg
     9001 aagtgctatt atggccatgt gcacggaaaa agcgggcata accatgcatt tcaggggatg
     9061 tatgaaggca cagaaatgcg aatgatttct agtgattatc tacaatttgt gccggaacag
     9121 attttctgaa ttgtataaaa tggagaagtg ttcaaaatat actggtcaaa acaggacaag
     9181 tatgctataa taaatttgta tgcattttgc atgaaaagat accctgatgc agagtgagcc
     9241 ccgatcatca gagggcacgc ttcaagggaa ttgcaataca ttnnnnnnnn nnacattatt
     9301 ggaggactca aacatgaatc tgaaatcatc aaacaagaca gatgtaaaca tgaccgaact
     9361 ggtcgttgaa atcgatgcag aaagctttga aaaggcagta gaagctgctt atcagcgtca
     9421 gaagaagaat atctctatgc cgggcttccg taagggcaag gtaccgcgga aaatgtgcga
     9481 gagaacttat ggcgaaaacg tattctatga agatgcgatc aacgctctgc tgaatatgga
     9541 actgccggct ctgatcgtgg acaacaagct gaatgtggta gataccccga aggtagaagt
     9601 aactgctgtc agcaaggaag acggcgttac catgaaggtc atctgcgtta ccaagccgga
     9661 gatctctctg gaaggctata agggcatcga ggctccgaag gttgtcaagg aaatcgctga
     9721 cgaagacgta gatcagcaga tcgaatccat gcgtcagaga aatgcacgcg tggtatctgt
     9781 agatgaccgt gcagcggaaa tgggcgacga agtaaacctg aactttgagg gcttcttcgg
     9841 cgacgaagca tttgacggcg gcaagggcga aaactatcag ctgaagctgg gcagcggtca
     9901 gttcattccg ggctttgagg aacaggttgc aggtcacaac atcggcgaaa acttcgatgt
     9961 taccgtaact ttcccggaag attaccagat ggacgaatat gcaggcaagg aagctgtatt
    10021 ccactgctct atcaacagca tttctcacga agaactgccg gaactggacg atgagttcgt
    10081 aaaggacact tctgatttcg atactgttga cgaactgaag gctgacgttc gcaagaagct
    10141 ggaagaaaat gcagaaaacg cagcttccaa ctctttcgaa aatgcagtta ccctgaagct
    10201 gatcgaaaag gtaaccgatc cgattccgca ctgcatgttt gaacatcgtg cagacagcct
    10261 gatgaaccag tttgcaaacc agctgaaggc acagggcatc tctatggatc tgtacctcca
    10321 gtacactggc atgacacagg acgcactgaa ggaaacttat ctggaacgtg cagaaaacga
    10381 agtaaagctg cgtctggctc tggaaaaggt tgcagaactg gaaaagctgg aagtttctga
    10441 cgaagatctg gaagcagaag taaatcgtct ggcagaagaa aacaacttga ctgcagaaga
    10501 tgtcaagaag cggatctatc tggaagacct gcgggaagac atgctggcaa ccaaggcgat
    10561 ggaactggtc aaggaagcag ctgttgcaac agacgctccg gaagaaaccg aagaagctcc
    10621 ggcagaagaa actgcagcaa cggaagaata agtacacact ggcatttttg ccgaaataga
    10681 agtgaaaaaa cagacgggcg aacgacttag ttcgccctct gtaatatgtg gacatttttc
    10741 gaaatgtgtc gatttggaaa ggaagatgaa catgagtagt ttagtacctt acgttgtaga
    10801 acagaccagc cgcggtgaac gttcctatga tattttttcc cgcctgctga atgatcgtat
    10861 catcatgctg tccgatcagg tcaacgatgc aactgccagt ctggtagttg cacagctgct
    10921 gtatctggaa ggacaggaca atgagaagga tatttctctg tacatcaaca gccccggcgg
    10981 ttctatcact gccggcatgg caatctacga caccatgcag tatatcaagt gcgatgtttc
    11041 taccatttgc atcggtatgg ctgcttctat gggtgcattc ctgctgtctt ccggtgcaaa
    11101 gggaaagcgt atcgctctgc cgaacagcga gatcatgatc caccagccgc tgatttccgg
    11161 cggtctgtcc ggtcagtgca ccgatattaa gatcgcttcc gaccatttgg tacgggttcg
    11221 gaagaagatg aacgaaattc tggcagaaaa caccggaaag ccgctggaag tgatcgaaag
    11281 agataccgaa cgcgataact atatgtatgc accggaagca aaggaatatg gtctgatcga
    11341 taaggtcatc gcacacagat aaaaagaggt gctgaaatgg acgagaaaaa ggaaaaacga
    11401 tgcaactttt gcggtaagcc ggagcatctg gtagaaggca tgatctccgc cgcagatgta
    11461 aatatctgta gtgagtgcgt gacttattgc tacgaaatgc tctatggtcc cgtcggcacg
    11521 ccggattccg caaaggataa gaagaaatct tccaaaaagc cggcagagat ccacctgctg
    11581 aaaccggcag agatcaaaaa ggtgctggac gagtacgttg tcggtcagga tctggcaaag
    11641 atcacgctgg cagtgtcggt gtataaccac tataagcgaa tcctcaacca gtccaaggac
    11701 gagtccgcac gagatgtgga actgcaaaag agcaacgtcc tgctgctggg ccccactggt
    11761 gtaggtaaga cttatctggc acagacgctg gcaaagattc tggacgtgcc gtttgccatt
    11821 gcagacgcta ctaccattac cgaagccggt tacgtaggcg acgatgtgga aaacgtgctc
    11881 cagcgtttgc tgcaagctgc tgatttcgat gtggaagctg cggaacgcgg tatcatctac
    11941 attgacgaga tcgataagat ctccagaaag tcggaaaacg tttctctgac cagagatgtc
    12001 agcggagagg gcgtacagca ggctctgctg aagatcatcg agggaaccgt ttccaacgtg
    12061 ccgccgcagg gcggcagaaa gcaccccaat caggaattta tccacgtgga tacccacaat
    12121 atcctgttta tctgcggcgg tgcgttcgac ggtctggaaa agctgatcca gaagcgtatg
    12181 gctccggcag cactgggctt tgatgctccc attgtgcagc agaagaaaga tctggtcaat
    12241 attttccgtc aggtaacacc gcaggatctg atgaagttcg gattggttcc ggaactggtt
    12301 ggccgtctgc cggtcatcac cacactggac gatctggacg aagaagcctt gatccgaatc
    12361 ctgactgagc cgaaaaacgc tctggtcaag cagtatcaga agctgttcga cttcgacgac
    12421 attgaactgg aactgacgga cgatgccctg aacgcgattg cagcagaggc aattgccaga
    12481 aagactggtg cccgtggtct gcgttcgatc atggagggaa cgctgatgga taccatgtat
    12541 accacacctt ccgtggagaa tgtcaagaaa atcgtggtag atgccgactg cgtcaagggc
    12601 actgccaagc cgagatttta tgatgcaagc ggcaaggaaa tcaccaatgt tccgcaggga
    12661 atctgaaaag gtgaggtttc tgccggctct gtcaaacaag attgagtgac atcttttata
    12721 aacagaaaac gcctgtctga tctcaatggt cagacaggcg ttttcttata tagatgtcag
    12781 cttattctcc gatattcttt ttcagaaatc gctccagcgg tgcggtaatg tacttgctga
    12841 agaacttcac tacgaaccct gtgcagaaag ctgcaatgac tgtaccttcc cgcgttccca
    12901 ccagcttgtg gaaaaacagg aacgagagaa tcactgccac cacaacacag ctgatgtcaa
    12961 aggcgatttt cacattgccg aattccttgt gaatggtatc cgataatgct ttgacaaatg
    13021 cctctccgga attcagaatc acatttgcaa tgaccgccag cgtaatgccg aatccgagaa
    13081 ccacagttcc tgccagtacc agcaaaatct gtaccacgta gtgcttcggc gtgaaaaagg
    13141 aaatcagcca gacaccgaaa tccgtaaacc agccaaacag aaaggacagc ggcacttgca
    13201 gcagctggat ccattggaac tttcgccgca gcagtaaaat ttgtcccaga atcagcacac
    13261 agttccagat gatcagccag taccccagcg acagctgggg cagccgaatg ctcatcacat
    13321 tcgccaccga ggagattggg gaaacgccca gttcgccttt tttggtaatg gcaactccca
    13381 gggcaccgaa aaatagtccg ataatgaata agagatagcg ttgtatcgtg tcaatcaaca
    13441 gagggctttt gattgtcttt tctttcaaat ccgtttcctc cagttttgaa attctgcggg
    13501 cacgtccgga cactgcatca tctgcgacat cacacagcag ccggcagcac cagcttgcgt
    13561 aatagcaggc agccgttccg gtgtaatgcc gccgatcgca tagaccggaa tagaaacact
    13621 gctgcaaaca ttccggagaa agtccagccc tcgcggcggc agatccggct tgcaggcagt
    13681 tgcaaaaatg tgtcctgcaa tcagccagct tgcccccagt ttttccgcaa gcaccgcttc
    13741 ttccggactg tgtaccgaag caccgatcgc cggaagctgt gttctctggg atttggtaag
    13801 agtacgcaga atcggcatgg gcagctgtac cggcagctgc aatgctccgg cgacttcccg
    13861 gagactgttc agccacagcg gcgtatgata tgcctgacaa atcgccagac actgtttggc
    13921 aagtgctgca tattccggaa cggacaaatc tttttcccgt agtaagatcg catcagcccc
    13981 gccggcggca agctgctcga ttcgttccag aaaatcaccg cggcagagtt ttcgattggt
    14041 aacgcagatc atcataagat attggtgttg atgtagtcgg tatagaccgg ctgcatgccc
    14101 tgtgccagaa tggcgttgtg gatttcctcc agacttctgg ggtcggagat ctcaaactgg
    14161 gcatcgcctt tctgctgatg ttcatcgttg tgtccgccga ttcccacatt gacccctgcg
    14221 gagatcttgg tggcagccag cggcagcaca tggtcacgga agtcggcacg ttcccgcgtg
    14281 gaaatggtga agctggcaaa gggcaggaaa atgcggcagg ccagcatcat ttgcagcagc
    14341 tgtgtttcat gcacgtcgtt ggggttggtt tcctcgtggt ttttataggg acgcagccgc
    14401 gggaaagaga gtgccatttc accctgcgga tacttctttt gcagcagtgt cgcatgcaca
    14461 ccggcggcga acatgtcctt gcggaaatcc gccagcccca gcagtacgcc gaagccgaca
    14521 ccacgcatgc cgcccatcag tgcacgttcc tgtgcattga cgcggtaggg gaaacaacgc
    14581 ttggcaccgg caacatgcat ttcaccgtac cgctgcaagt cgtaggtttc ctgaaaaacg
    14641 gtgacgaaat ctgcccctgc ttcccgcagc atcgcgtatt cgtcagcatt catggggtac
    14701 acttccagtc cgatcgtgga gaaatatttc ttggcaatgc ggacagcttc cgcaatgtat
    14761 tccggaccgg aaacagaacg gctttcaccg gtgagcagca gtacctcccg catgccggtt
    14821 ttggcgatgt tctccatttc cgcttcgatt tccgctgcat tcagcttgca gcgggtaatg
    14881 ggattcttgc agttgaagcc gcagtaaatg cactggtttt cgcagtagtt ggagatgtac
    14941 agcggtgtga acagcagcac attgttgccg aagtgctttt cccgttcttc ctttgcccgc
    15001 cgagccattt gttccagaaa aggctgtgcc gcaggggaga gcagtgccgc aaaatcctcg
    15061 aaagacagca catcttttct caatgccgcc tgtacctgct tggctgtata ctgttccggc
    15121 tgatatgccg ttctggcagc gatcacatgg tcaaaaatgt cagaatcgat ttgctgcatg
    15181 ttgggctgat attccatagg gtcataaatc ttacgcattc cgcttcctcc ttaatcctgt
    15241 aaaaagccgg tcagcgggct ggaagcctct gcaccggatt ccagcactcg gcctagcttt
    15301 gccagatatg ccgcacgtcc agcctcaatt gccagcttga acgcgtgtgc catcatgggg
    15361 cagttgtcag cggttgccac agcagtgttt gccatgaccg cagcggcacc catttccata
    15421 gcttcgcaag cctgcgaagg agcaccaatg ccggcatcta caatgatcgg caggtcgatc
    15481 tcgtcaatga ggatttggat gaagtccttg gtacgcagtc ccttgttgct gccaatagga
    15541 gcacccagcg gcatgatcgc agcagctccg gcatctgcca gacggcgtgc cgcgatcaga
    15601 tcagggtaca tgtacggcaa tacgtggaag ccctctttgg acaaaatttc tgttgcccga
    15661 atggtttcgg cattgtcggg gagcaggtac ttcgtatcgg gaatcacttc cacttttaca
    15721 aaatcgccgc agcccagtgc acgggaaaga cgggcaatgc gaaccgcttc gtctgcgttt
    15781 ctagcaccgg aagtgttggg cagcagtgtc aggtttttcg gcaggtaatc cagaatattt
    15841 tccggactgt cggcattggc acgccgcaga gcaatggtgg caatttcgct gccgccgtac
    15901 tccatgacat cacggatcag ctgctgggag aacttgcccg agccgagaat aaagcgggag
    15961 tggaattcct ttccacccag tacaaaagta tcatttttga tcgtttccat aagaatcttc
    16021 ctttctatca caacatcaca tatttgcggt cagcatgcac cgccgcccat aaagctgacg
    16081 atttcaatca cgtcgctgtc ttggagaagg atcgtggcat agtcttcttt tgcctcgatt
    16141 ttctcgttgc gttccacggc aatgcggtgg agattgtacc ccttctcgtt cagaaattcc
    16201 agtaatgtct gcggtttttc cagagggatc gaaacaccgt ttacctgcat ggcatacact
    16261 ccttttctac attttaccat aaaattgcac aaaaaaaggg cacacacctt ttcggtggtg
    16321 tgccccattt tacagtgatg atattctttt cgaataccct tagtatagca cattttgcag
    16381 gaaaagtcaa cgctttttcc tgctgtgtag aaattttacg cttttgccat gaaatccggc
    16441 agaaagtcca caaccggtag gtcatacatt tttgccaagt ccagacaatt ctgtatatat
    16501 gcgacgttgg aaagccggtc aggcgtgtgg gcatcgcagc cgatgatcgc agaatttccc
    16561 gctttctgtg cgatgcggag aaatctgccg gaggtgtagt gccggtgttc cacaacgccc
    16621 agcaggttga tctccaccgg aatgtcgtgt gctttcagat actggcagag ccggagcatg
    16681 tgccgttcgt agatctcgtc cgagccgatg tagtggatca ggtcggggtg agccacatag
    16741 gcatattttc cggtttccat gccctcaatg acaaggtcta cataccgctg caaatccgcc
    16801 tcggattctg tttcatgacc agtataggga ccggtgactt cgctggcagt gaagtgttct
    16861 cccagcagca tgtaatccac gggataatcc ttcagcagcc ggtcctgttc ttccatcagt
    16921 tccggaatat attcggactc aaagccgatg taaatggtaa tgtccttggc atattcccgc
    16981 ttcaggtttt ccagcgaggt gaagtagccg tccagttctt tcggttccat gcgggtgtgg
    17041 gaaacatagt ccggcggaaa gatccacgga cagtggtcag aaaatcccag cacttccatg
    17101 cccgcctgaa ttgcctgttc cacatactcc ttgtcttcgc cccatgcatg cttgcagcgg
    17161 gcagtgtggg tgtgataatt tgctttcaac atgaatggca tacgctcctt actgttcttc
    17221 cggtgactgc ggggcttctc ctgcggcatc tgtatctgca tcagtgatag tggtttctgt
    17281 aaagctggtt tccactacgt tgccggagta cttgggagcc agccgtgcaa cgatttcgtt
    17341 gatcattcgc agcacgccgc tgataatcat cagaatgcca atgatgttcc agccggtgtt
    17401 ctggttgatg aagatcacat aaatcagaat gaaaattccc agcaggcagt tggtgactgc
    17461 aaagagcatg gaagccacaa tgttggtgtc cgctttgcgg aattccattg tgttgacgat
    17521 accggaaatg ccgttgcaca gcacgcagat gcccagaatg atcgggatca gcttgttgat
    17581 ggtaaacggc aggataatga gcagggcacc gagaatcagc agaatgatgc cggtcgtcag
    17641 tgtcttagcg ggctgcgggg gctggcactg gcggcgggaa gcatacacta ccacttcgat
    17701 caacccgatc acggcaatga gaatgcccag cgtggtgaag ataccgttca ccaatttctc
    17761 gttgacctgt agcagaatgc ccagtgcgat caggcacagc ggcagcacaa agggaaatat
    17821 cacatagaac atgcgtttgg aaaatggtgt ttttttggat tgcatcagta aaactccttt
    17881 cgcatcaggg aaacactgaa taaagccgtc aggcgggctt tatgcagtgt ttcctcaggg
    17941 caataccgca caaagagcgt ctggcgactt tgcacgccat ctgcctgcaa atttcccgtc
    18001 atatttcaca aaaatgctcg tgaatacatc aagtattcac tccgcttttt gtttcatctg
    18061 acgaaaaatt tgacgacgca tctgtcgcac aatctttgtg cggtattgcc tcagatcaga
    18121 tgcagtaaat atttcataca aaaccgcttc agcggattgc atcgtttgaa aatggcatct
    18181 gccagagcag aagcctctgc ggcagcttgt cgctgttctg ccatttccag attctgtttc
    18241 ccgaattcca ttgccagaat catgggaatg atctgttccg gagccgttgc aggcagtttc
    18301 tgctgccgga gatgctgcac tgccttttcg cagaattcca gcagcggctc ctgccggaac
    18361 cgcagtcctg tccaacgcag aagccggaac agatagcggt acatggcatg aactgcctgc
    18421 cgcgggtgac gggtatccag aaagacattc tgccggtatc gaacatggag cagccgcagc
    18481 aaaaccgctg ccccaatgaa aagcccgatg cagatcgccc agatcagcag ccgcactgca
    18541 accgcagcga agccgggtat cgaaccgtcg gcgggtttgc tgccggcaat ggtgttgtct
    18601 tttggagccg ccatgctgcc ggaaatttcg gtagaggtca gtgcggcact ggtcatctcc
    18661 tccggcgttg cagaggtgtt gttttccggc gtagtatcga cagtggtttc cgtggagggc
    18721 tccgacggta cttcctctgt gtcgctgtcc ggctgtagct ctgcggtgta gccgggagtc
    18781 atttcaaagg gcagccaacc gtagccgtca atgtaaaact cacaccatgc gtgagccttt
    18841 ttgttgggga cgttgacgac ataacgcccg tcacggatag aagcactgtt caggacttcg
    18901 ctgtccacca gatacccttc gcaatagcgt gcgggaattc ccaggtgccg tgccagaatg
    18961 gcgccggcgg tggcgaagtg catgcagtag cccttgtgat tttccagcag gaagaagttg
    19021 acatagtcgc ggtttcgcgg cgtttttccg ggagcaaggt cgtactccgc ctgctccgag
    19081 agccacgccc gaatctcaca tagtgccgca tagcgttcct gggtggtctg cgggtgagcg
    19141 ttttgcagcg attccggcat agcggcatat accgcatcca gggcttcgct ttccggcagc
    19201 cttgtataca catctgcggc aaaatcacgg taggcgacag attccagaaa cgccgccgga
    19261 attacttttc cctgcacacc gtcaggagcc gttgcaattt gatttgccga aaaccgcgtg
    19321 gagatcgtca catcagagcc gttgctgggc acatgcagaa tgtccataaa agaccgtacc
    19381 gcatcattgc tccacgagga attaaacgaa atcgaagctg gttcctgctg tgagagcacc
    19441 gacaagatct ccggcgaaag ctgtgccgct gtccagtcgt agctgtcgcg gtttttcgga
    19501 cgaaaatcga catcttccag ataggaaaca tcagacgaaa atgccgcata gggctgataa
    19561 tatctgccgt cccgccgctg cggggtaatg gtgcagtgat acgtaccgga atccggctca
    19621 ctggtctgct gaaacaaaaa cggaaaattc tgcgggttgc agtcgtagct ttccagtatc
    19681 ggaaagacca tatcgctgtt gaccgcctct gtatctgaag ccagtgtgtc ccagcagttt
    19741 ttcccatagg tgctgccggt atagcttttc agatacatgg tgctttccgg cagatcgtcc
    19801 agcgtcagct tcagatctgt ccggtcgtat atccgctgtt tggcagtgtt tccgagggtg
    19861 acagtctggt tgttgcccat gccgattgcc gcagcgatct tgcgaatgct ctggggcaga
    19921 ttctgaatgt cgaaatccgc cagactttca ctgatctcct gccgccgcag cttgtccgat
    19981 tccggacggc tgtacccctg ccatgtgcct gtcagggaaa ctgttccgta taccagcaga
    20041 atcagcaatg ctgtgagaga accgccgatt gcggaggtac gctgtgtgca gcggatcttc
    20101 tcccgtcccc acagtgccgg aaaccgcagt gccagcatgc cgccccagta tgccagaagc
    20161 agtcccattg ccggaacgct gacagggatt cccagataca gccccggctc tgcaagcagg
    20221 aaagaaactg cggtaggcag tgtgaaatgc ggctttcgaa tggtgaagaa ggcaaagaga
    20281 aatgccagca gttccgccac aagtgtgccg gtcagcgttg ccgcaaaagt gatgtttgcc
    20341 cttttggcaa tctgaaaata tgcgatattt gtattgtgag ccgtctggta gaccacattg
    20401 aaaatggcat gaaaacccag cacgatctgt ttggaaaaca gggcagacat gcccagaagc
    20461 accagaacaa aacccagtgt cagctttccg ctgtgcttcg gcaaaaactg gagcagggaa
    20521 gccagcagga aaccgccgac tgccagcggc agaaagattc cttttcgaat gtttgtcccg
    20581 tagaggtcgc agatgaccag cacagagccg aaatacccca gtgctgtcag gaacaattgc
    20641 agcagcttcg ccgtgttccg cttggtcggg ttgtccagat catatcgtac cgcttcttcc
    20701 tcgattttca gagaacaggg gacgtgtgtc tttttcatgc tgcacctccc tcggaatctg
    20761 tcagtgtccg atagacgatt tctgccccgt tgccggcaat ggaaacgtgt ttgtccgagc
    20821 cggcagcttt cgcagtaaac actttctgaa acggcggatt ttccagaatc gtatctgtca
    20881 cacagaacgc cgccgtttct ccaaggctct cccagtggga aaggcaggcg gcagcatcgg
    20941 cttccggcgg tgtttccagc agattttcca acagcggcag cagggactgt tcctctgtaa
    21001 tggtttcgct ccggtgccag tcagaacggc agaacaccac cgtcacacca tcttggttgg
    21061 cacagaaaaa ggcagtacac gcctgcaacg cttccagcat agcgtccagt tcctgcccgc
    21121 gttcctgcgg gttatctgcc tgcacagcat agagaaatgc caccgtcacc ggcgtgcgtg
    21181 caggaatgcc gtactcccgc accactggtt ccggaaagcg gctgctcagt ttccagtgaa
    21241 tggcacgcat gcggtcgccg ctgcggtatt cccgtacacc cagaaattcc tccggctccg
    21301 tggtgggctg catcactgca tcttcgccgg cttgcagcgg agatgtactt gccggcggct
    21361 gtggcacttc cggcagggca gtcgcagaaa aggcggcgga aaaccgcttg cggtgagaga
    21421 acagcagcag ggtgtccagc acctgaaccg attgcagggt aacgtgaact gcaccggcat
    21481 gctccggcgt gaccggcaca tggatacaga cagtctgccg cggcggaact gcttcgtgaa
    21541 attcaacatc gtccagttct gtcagcgttt cattttccag atgtatgcgg aaaatcacag
    21601 tgctgatagg aaatatcgtg ggatttttca gccgcacaca gcaggaaaac ggctcgccga
    21661 tatgtgcatg cggcttcggc ggcaccagtt ctgcctgtaa ctttgccccg ctgtaatgtg
    21721 ctgcaaagaa aagcaacgcc ggcaaaagca ggatacacag cagcaaatac gccgagaact
    21781 ggggcagata cagacaaaag aacaaataac aaacgccggc aagcagcaga tagagaattt
    21841 tcagtgtccg catcgcgttc accgtctgtc cggcatggga gccggtacct gatgcagcac
    21901 ctcgtccagc acttgctccg ctgccacgcc gcctttggtc aggattcggt gtgccagtac
    21961 gtcctgacac aggggcatga tgtcgtccgg cagcagataa tcccgtccgc ggagcattgc
    22021 cattgccttt gccgactgga tcagtgccag cgttcctctg gggctgacac cgaggacagc
    22081 ctgcgggtgt tcccgtgtgg ctctggaaac cgaagccgcg tagtaataca ggcggtcgtc
    22141 cacatagact gccgcagcct gctgctgtaa tgcctccagc tgctccggtg tgcacaccgg
    22201 ctggacatct tccagcggat cgcgggcggc atgtcctttt aaaattgcca cctcgtcctg
    22261 aatctccgga taccccatgt gcagccgcat cagaaagcgg tccagctgag attccggcag
    22321 ggactgcgtg cccacacagc ccagcgggtt ctgggtggca atgaccagat gcgggcgggg
    22381 cagggcgtag gtcttgccct ctacagtgac gttgctctct tccattgcct ccagcagagc
    22441 tgcctgtgtc ttgctggagg tgcggttgat ctcgtccgcg aggagcaggt gacaaaaaag
    22501 tgctcccggc tggaacacga atgttccggt atttttttca tagaccgaaa agcctgtcag
    22561 gtcggagggc agcacgtccg gcgtaaactg tacccgacgg cagttcagcc ctagagcctt
    22621 ggaaaacgcc atggcaaggg tagttttccc gacaccgggc atgtcctcca tcagcacgtg
    22681 tccgcctgcc agaaatgcaa gcagtacttt cgtcaaaacg aggtcttttc cgataacagc
    22741 tttttgcacc tcccgcagga tcgcggcggg gagtgtacga gttgtggtaa ctggcataat
    22801 tttctcctgt tccggcgagt atgtgccgcc ggcttcacat ctttttcatg atatatgatt
    22861 tattatatca ttttttcggg gtttttgcaa gcgttctgtc tgtcttttcc gcaaaatccc
    22921 cgcgtgtttt tttgtttgct ttcggtaaaa acggcgaaaa aacaaaaatg cggaaaaaaa
    22981 gcagcgggag gatattgaca aaaccacaaa aaatgttata ctagaataag agcggcacgg
    23041 cagaaagt
//
LOCUS       DAWBGR010000016        21911 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_847_length_21911_cov_4.811951, whole genome shotgun sequence.
ACCESSION   DAWBGR010000016 DAWBGR010000000
VERSION     DAWBGR010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21911)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_847_length_21911_cov_4.811951"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5518 a         5918 c         5507 g         4968 t
ORIGIN      
        1 caagcagatg gcgtgcaaag tcgccagacg ctctttgtgc ggtattgcct ttactcctac
       61 ggcggcaaca cccgccagat cgccaagctg gtacagcaag aaaccggcgg tgacctctgc
      121 gaaatcgaaa ttgcaacacc ctacaccggc agctacaacg acgtggtcga gcagggacag
      181 cgggaagtca acagcggttt tacgccaaag ctgaaacccg tttccgcaga cctgaaaaat
      241 tacgatactg tgatactggg cacgccggtc tggtggtaca cctacgcacc agcaatgcgg
      301 acactgctcc agagcagtga cttttccggc aaagtgatct atccatttgc cacgaacggc
      361 ggctggctgg ggcacacatt tgccgacatt gccgaagcct gccccacagc cacggtaaag
      421 gcagggctga atattcgatt caacgaagac cgtctggtca caccggaggc tgagatcctg
      481 cggtggataa agggcatttc ggagaagtaa aggaaacact gaataaaggc agtgctatac
      541 aaaacagtgt aagacaaaac ccgacttctg cagctgtatg cagaggtcgg gtttttaatt
      601 gcggatatgc tataaaagaa tcactttgcc agaatttctt cccacgattt ctgcatgccg
      661 gctccgtgaa gtgccacgta atagagcatg tagtagatgt ccgtggaatc taccgtgcca
      721 ttgccgtcca catctgcggc agcgatctgc ttggcactca attcaccgtc attgcctaca
      781 gcaatgtttg ccacatagta caaaacatag tagatgtcgg tggaatcaac atcaccattc
      841 ttgtcaatgt cgccggcaac tacatcggca ggctttgtca cagtgccgtc agcaaaatga
      901 atcgcagcag ttactaagaa gtaattgtca taattatcag tggaattgcc gccggtgatt
      961 gcaattgcat tccactgttc cggcgttccc gcatagtaaa catctgacaa gttcatacaa
     1021 ttgcaaaaaa cagtttctcc tataaaggtt acacttttgg gaatagagat gctggtcaaa
     1081 ctataacagc cataaaaagt tactgcgtca atactggtta cgccctccgg aatcgcaatt
     1141 tctttcaaag tcgcacagtt aaaaaacgca gatgagccaa tactgttcac actatccgga
     1201 atagaaattt ctgtcatttg acaatttgca aaagcacctt ccgtgatgct ggttacccca
     1261 tctggaatcg tcaccttgcc ggaacactgg ctataatcca catcaactaa aatatggttg
     1321 atgatcacaa acggattttc atcatatcga tacgttaccc atgcagtatt cgtaaagact
     1381 gactctccaa aaaatgtaac cgaatccgga attgtgatat tttttaaatt tccacagtca
     1441 gaaaaagcat aactgccaat actggtgaca ctatccggaa tcacaacgct cgtcatatga
     1501 ttttcccaaa aagcaaaacc acaaatactt gttacatcat tcggtatgac aatttcttca
     1561 tcagtacaag tcgttccgtc cagcaaaata ccgtttacaa ttaccagagg attttcttcc
     1621 tgtcttgcag ccagccaagg cgtatccaga aaagcatttc cgccaatgct ggtcacactg
     1681 cttggaattg taatctctgc caaactcgta caaccatcaa aagcactata tccgatattt
     1741 tctacgctat tcggaatgat aacactcgtc agattctcgc aacctgcgaa acttctatca
     1801 ccaatgttcg tcacactact cggaattgta atgttttcca gtttttcgca attaccaaaa
     1861 gcattatctc cgatatttgc tacaccattc ggaatgatga cactcgtcag attcgcacaa
     1921 tagctaaaac tgtaatatcc catactcgtc accgttttcc cgtcaatctc tgccggaatt
     1981 tccaagtccc ctgccgcatc attttcacaa tctgtaattt ctacagtatc atcggcattg
     2041 acagtatagg tcagctgcgt tgttcttgtt cccaccggaa ctgtcactgt gtacgcagta
     2101 tcattttccg ctgcacacac cggcagcacc gcactgacac ttcccagcac accctgcaag
     2161 ccggatacgc ctgcacagcc cagacagagc atgccggctg tcactgctgc gagcactttt
     2221 ttccataact tcattttcat tcctccgttt ctattccatt gcaaaacatc tgcattttta
     2281 ttatagcaca tatgcttaaa aaagtcaatc tacttttcaa aaatttccat ttttgttttt
     2341 accttgacga accgctgtga atatgctaca atacaaataa aaacaaccac cacaacagag
     2401 aaaggacgca tcatggatcc acagacacaa ccacaaaaca ataacactcc gcagcaacca
     2461 aatcaaccac cgcaaaatgg ctacccctac cagcagcctt atggacagcc gcaaaacggc
     2521 tacccctatc agcagcagcc ttacggacag ccacagaacg gctactccta ccagcagcag
     2581 ccttacgggc agccacaaaa cggctacccc taccagcagc agccttacgg acagccacaa
     2641 aacggctacc cctaccagca gcagccttac agacagccac aaaacggcta ctcctatcag
     2701 cagcagccct atttcccgcc ggcacagccg cccaagcagg gggcaggacg tgtggcaaac
     2761 tttttctttt ccctgctgcc aatgctgagt tttttcgcca tacagatctt tgcttctttt
     2821 gtgatgatct tcctcatgat tttcgttgcc ctctgccgta ctgacggcaa catgcaggca
     2881 gtcctgccgt attatgaaag catcgtctat agcagcgttc cgaccgccac ggcactgtcg
     2941 cacctgctgg cagccatcgt ctttgcgttc tggtatcggt tcagcttccg gaaaccacgc
     3001 cccgccatac gagaaaccac acagaagctg aaaccggagc cgattctcat ggcactggga
     3061 ctgggcattc tcatgtctgt tttcagcagc ggcacctccg gcatcgaaag cctgctgttc
     3121 ccgcaaatgg tcgaggacac ctacgaaatg atggagcgtt ccggcatcgg caccaatggt
     3181 ctggtcatct ttgccactgt ctgccttgct cccattggag aggaactggt tttccgcgga
     3241 ctgaccatga aatatgcgga aaaagcattc gggcggttct gggcagccaa tctgctgcag
     3301 gcgtttctgt tcggcgtgat acacatgaac tgggtacagg gcgtgtatgc attcttcggc
     3361 ggactggtgc tgggctgggt cgctcaccgc tatcgctcag tggtattctc catgctgatg
     3421 cactttgctg tcaacagctt ttccacgttc ggtggggact atctcctcgg ctggatcccg
     3481 ctgaacttca tcactgctac cctgctggtg gtaattcctg cggcaattgc aatcggtctg
     3541 attgcatggc gaaatgatgc cacggagaaa gctgccccgt ccggcggaac actgccgccg
     3601 cagggacagt gctaacaaac cacaatattt ccgaaaccgg tttctgtaga tacaggagcc
     3661 ggttttttgt atggacagca ctgcggaatc cattgacttt ccttttgctt catgctataa
     3721 tgagaacact gcaaaacgtc aatatgcagt gtcctaaaac gcaaaaagaa atggagaata
     3781 cacatgaaat tatggaaaaa agttctggca gcaacggctg ccagtgcatg ttgtctgagc
     3841 tttgcccaag tatccggatt ccaaaacctg ctgcaaactg ctgaaccggt gttgtcggca
     3901 agtgcagctt ccggcactta tgaagatttg acctattctg tcaacagcag cggagaaatc
     3961 accattaccg actgcgacaa aacggtaact gctgtggaaa ttccggcgga gatcgacggc
     4021 tgccctgtaa ccagtatcga aaatgcagcc tttcggcggt gttctaatct gactgatatt
     4081 accattccgg aaagcatcac ctatattggc aaagaggcgt tttcctatac cccgtggctg
     4141 aaagcacgac aggcggaaaa tccactggta attgtcaatc atattgttat cgacggacag
     4201 acctgcaccg gagatgttgt catacctgac ggggtgacca gtattgccaa ctatgccttt
     4261 tcttccgact tgggactcgc aaccgcctgc tctacacaaa tgaccagcgt caccataccc
     4321 gacagcgtga cacgcattgg ttcctctgcc ttttccggct gcaaggggct gctccgtgtc
     4381 gtactgccga aaagtattac caccattgaa gtggcatctt tctatcagtg ctccagcctg
     4441 accacgctca ccattcccca aggcgtgacc acgatcaaaa gcaacgcctt tgccgcatgc
     4501 accggactga ccggtctgtg tctgccggac agcctgacaa aaatagaaaa ctgggcgttt
     4561 gcttcctgct ccaatctaac ccgcatcacc gtccccgcaa gtgtgaccag catgggagaa
     4621 tctatattcc gagaatgcag cgggctgacc attcggggat atgccgactc cactgcccag
     4681 agatacgccg aaaaatacag catcgccttt gctgacctga acaatcctga caccctgctg
     4741 ggtgatgtgg acaacagcgg cagcattgat tccacggaca tctactatgc cctgttccac
     4801 gtggcaaata ttgcggtagg caatgacagc gggctggaaa tacagcagat cgctgccgca
     4861 gacatagact gcaacggggc agtggattct accgacatct actacctgct gtactacgtg
     4921 gcactccacg gggcaggact tgacaagtcg tggagcgagg tactggcgaa gtaatccaca
     4981 cagagaaaat ccttctgcaa caaaaaattc attgacaaaa ccagtgaaat cgcatataat
     5041 aataaggaaa tcctattgca cagtatgatt tgtgcataca ggcagatgat aaaaacaacg
     5101 cagcagtgag agtatctccg gcagcggtgt gcagagagca ttgggtcggt gaaacaatgt
     5161 cccgcaccgg agggaagtgc aactgcgacg ggaatccgaa tttttcgtat tccccgttgg
     5221 catggggaac ggcggtttct gtgtaagatc gcccgaagta tccgtgtccg gcctgccccg
     5281 ttatcggcat gggaactgat tttttgcgta attgcgtaat agaaaaccag ttccaaggga
     5341 ttttctctat agaaaaatcc cgttgagtga gaaatcagag tggaaccgtg gaatattttt
     5401 tccgcctctg gaagtgtatg aaaaacgcac ttgcagaggc gtttttttac ccctgcaagt
     5461 accgacaatt ttaggaggtg acatgggaag atgtttcgac agcttcggga agatattgcc
     5521 attgtcaaag agaaggaccc tgccgcacgc agcagtctgg agattctgct gacctattct
     5581 ggtctgaaag cagtccgcag ctaccggcgt gcccactggt tctatcagca taaatggttc
     5641 accattgccc gcatcatatc ccagcgggca cggcacaaga ccggcatcga gattcacccc
     5701 ggtgcacaga tcggaaaagg gctgttcatc gaccacggca tgggtgtcgt gatcggggaa
     5761 accaccgtga tcggtgacaa ctgtctgctg tatcagggtg tcacactggg cggcaccggc
     5821 aaagacaagg gcaaacggca ccccacactg ggcgacaacg tcatggtggg tgcaggtgcc
     5881 aaggtgctgg ggccgatcaa catcggcaac aatgtcaagg tggctgccaa cgccgttgtc
     5941 ctgaaagaca ttcctgataa ctgtaccgcc gtaggcgttc cggcgagaat cgcccgcatg
     6001 gcaggacaaa aagttcagca gcaggatctg gatcagatcc acattccgga tctgaccatg
     6061 gaggacatca ccatgctgaa atccgcagtg acctgtctgc ggacggagat gtctaagctg
     6121 gaaaaacagg aacagacgga gaaaacggag gaatgatcca tgcagtgtgt ggtatttgat
     6181 atggacggcg tgctgtttga ctccgaagcg ttggtgcttc agtgctggga acagacggcg
     6241 aaactccacg gaattccgga aatacaggag atctgccacc ggtgtctggg cagcaatgcc
     6301 gacatggccc gacagatgtt tctggagaaa tacggagccg attttcccta cgacacatac
     6361 aaggcggaga tgtccgcaaa gtactgggag aacgtggaag ccggcaggct ggaactgaaa
     6421 tccggtgtgc gggaaatgct gacggcactc cgtgcccacg gctggaaaac cggcattgcc
     6481 tcctccacac gggcagtgat agttcggaaa gagatgcggc atttcggctt ggaacagatg
     6541 ttcgatcaaa tcatcggcgg cgacatggtg cagcacagca agccgaatcc ggagatttac
     6601 cagactgcct gtcgggaact gggcgtactg ccggcagagg cttatgcggt ggaggattcc
     6661 tacaacggga ttcgctccgc cgccgcagcc ggcatgcagg cgatcatgat tcccgacctg
     6721 ctgccgccca caccggaaat ggaacagctt gcggcaaaaa ttttcccgtc tatgggagat
     6781 tttcaacaat ttttgtttgg ataagccaaa ccggaacggc actgccgttc ctacataatg
     6841 tgcatatatt atataatagt ataaaggagc agaacggtca tgcagatcta taacacgctg
     6901 acacgaaaaa aagaagaact cgtccccatg acagcagggc acatgggcat ctatgcctgc
     6961 ggtcctaccg tctacaacta tatccacatc ggcaatgccc gcccgatctg tgcattcgat
     7021 gtgctgcggc ggtatctgaa gtaccgcggc tacacggtca cctatgtgca gaacttcacc
     7081 gatgtagacg acaagatcat caaaaaggca aacgaagaag gcaccactgc ggcagaaatt
     7141 gccaagcggt atattgccga gtacaagacc gatgcccacg ggctgaacgt catggacgct
     7201 gatgtacacc ccaccgtcac cagctgcatg gatctcatca tcgacattgt aaaaaagctg
     7261 gtagactccg gtcatgccta tgccgctgcc aacggagatg tttacttccg cacttcctct
     7321 tttccggaat atggcaagct gtccggtcag cctattgaag aattgcaggc tggtgcccgc
     7381 atcgacatca acgacggcaa ggaagaaccg ctggatttcg ccgtgtggaa agctgccaag
     7441 ccgggcgaac cctactggga atcgccgtgg ggcaagggca gaccgggctg gcacattgag
     7501 tgctctgcca tgtcctgcca ttatctgggt gaaacctttg acctccactg cggcggtcag
     7561 gatctgatct ttccccacca tgaaaacgaa atcgcacaga gtgaagctgc caacggcaag
     7621 ccatttgccc actactggat gcacaacggc tatatcaaca tcgacaacaa gaaaatgtcc
     7681 aagtcgctgg gcaatttctt caccgtgcgg gaagtggcag agcagtacgg ctatgaagtc
     7741 attcgctaca tgatggtgca ggcacactac cgcagcccca tcaactactg caaggaactg
     7801 ctggacgcct gcaaggcatc tctggaacgg ctctatgtct gccgcgacac actggatcgt
     7861 gccattgctg cggcaaagtc cggcgagatc tccgctgaag caacgaaaac tttcgccaag
     7921 cggaaagaac agttcatcac tgcaatggac gacgacctca acaccgcaga cggcatgact
     7981 gcgatctttg aactggtacg cgacctgaac cgcatgagtg cagatgcttc cacctccaag
     8041 gagcagctgg aagccggtgc aaagctgttt gacgagctga ctggtgtcat gggcttgctg
     8101 tacaaccgga aacaggctga tacaattccg gcagaagtca ccgcactggt tgaacagcgg
     8161 caggctgccc gcaaggcaaa ggacttcgcc gaagcagacc gtctgcggga caagatcgct
     8221 gcactgggct atgcagtacg ggaaaccaga cagggcactg aaatcaccaa gatcgacgcg
     8281 taactgcgtg agaatccact ccggaaagga aagctatggc actcaaaaac cataaaaaga
     8341 ccattacccg aatttttatt ctgctggttg tgatgacgct gatctgcatg ggcgtgttcg
     8401 cctgcaccaa ctatctggag cagcagagca aggcgtttgt ggacatgagc aaggtgcagc
     8461 tgatccagtt ggacgaaccg gaatcagacg ctcctgccat gaagatcact accaccgccg
     8521 gcacgatcgt ggcagaactg tttccggaac aggcaccggc gtatgtaaag cagttcaccg
     8581 aactggcaga atccggctac tatgacgaca cctatgtgtt ctcggtggaa aagggtgtat
     8641 acttcgaagc aggcagcccc aatgcagacg gctcgctgga cagcgatgca gacggcacct
     8701 atgaaaaagt agaacgggaa accagcggcg acctctggcc gttccgcggg gcattctgcg
     8761 tgccgacgac ctccaaggag ggcaacattt tagaccgctt caccggcaga atgacctcct
     8821 actgcggtac ccgctttgta gtgtgcaatt ctatcgtttt cgacgattcc accaaagagg
     8881 agttgcagtc ggtttccgaa aatgcagaaa aaatcaacga tgcatttctg gaacgcggcg
     8941 gcgtgccgaa ctactcccag cagatgactg tctttgcaca ggcttacggg gacgaaagct
     9001 tcgataccat tgacaagatc acagaagcag cgacccagcc tgccgctacg gagggcggct
     9061 acaccccgcc ggaagatgaa atccgcatcc agaccattga gatcggcacc tatggtgaat
     9121 ttgcagacac cgccgaaaac acaaaaaaat gacgggaaat ctttcgtttt tgaaaaatac
     9181 atatttcccc ttatggattc catacaaacc agttttcaca taggagaaca tttccgtcga
     9241 tacttccaaa taacgcgaaa aaagtttggt ttttacttta caaagtatca gaattcgtgt
     9301 ataatgaaga agatagcatg caaactatgt ttgtatgctc accggagaat cgctccggaa
     9361 atggaagtca accggcagca attttcactg ccggaaacag tgcggcatgc ctgcatgcca
     9421 atgacacatg ccgcacgaaa tcgtggaacg gggacgacct gcgacaagct gctgcgggaa
     9481 tgtccctgat gtgccgccgt atctgggtga taccggttcc tttcccgatg tcatcacagc
     9541 aatggacaat tgggtggcac agaaaaaagg agagacgaaa ttggataaat ttgtcattca
     9601 cggtgggcat cggctcaccg gcgaaataga agtcagcgga gcaaagaatg cagcagtcgc
     9661 actgatccct gccgtgctgc tctctgatga gccttgtgtt ctggaaaatg ttccggacat
     9721 cagtgatgta tccatcagcc tgcagatcat gagcgaaatg ggtgcaaccg tagagtatct
     9781 gaccaagacc accgtccgca tttcagcggc aggtctgacc aattattgtg taccctatga
     9841 gagtgcacga aaaatgcgtg catcgtatta ttttctgggt gcactgctgg gacgctatgg
     9901 caaggcgaga gtttccatgc ccggcggctg tccgttgggc gaccgcccca ttgaccagca
     9961 tctgaaagca tttgcggcac tgggtgccaa gtgcaatatt gaacacggca tggtagatct
    10021 ggtggcagac caactgacag gcaatcagat ctacttagat gtggtttccg ttggtgccac
    10081 gatgaatgcc atgctggcag cagtccgtgc caagggtctg actgtcattg aaaacgccgc
    10141 caaggaaccg cacatcgtcg atctggcaaa cttcctgaac tccatgggtg cagagatcat
    10201 gggtgcaggt accgatgtca tcaagatccg cggcgtagaa cggctgcacg gtgcagtgta
    10261 ttccgtcatt ccggatcaga tcgaagcggg tacgtttatg attgctgccg ccgccaccaa
    10321 gggcgacgta ctggtgacca atgtcattcc gaagcatctg gagccgatca ccagcaaaat
    10381 gcggaagatc ggtgtcaata tcgaggaatt tgacgacagt gttcgggtat gggtagacgg
    10441 tccgctggtg cgtaccaacg tcaagaccat gccgcacccc ggctttccca cagatatgca
    10501 gccgcagatc accaccctgc tgacactggc agagggtacc agcatcatca ccgaaggtgt
    10561 ctgggaacag cggttccgct atgtggacga acttcgccgc atgggtgcag atatttccgt
    10621 ggacggcaag gttgccgtag tagagggtac cggaaagctt accggtgcac cggtgaaagc
    10681 ctgtgatctc cgtgcaggag ctgccctgat cgtggcagga ctggctgcac acggcgtgac
    10741 cgagatcgag gacatctatc acatcgaacg cggatacgcc aatctggacg caaagctgcg
    10801 gcagatcggt gcagatattc agaaggtttc cgatccggca ataccagcga agctgaaaga
    10861 agcactctaa gccaaaacat cattcaaatg caaaaagccg tcagcttcgt tggcggcttt
    10921 tcttgtggaa aagaggagat ctgatcttgt cgaaatttta ttctctgttc agttccagta
    10981 agggcaacgc ctcctttatc ggcagccctg ccggcggcat gctggtcgat gccggagcca
    11041 cctgccgtaa actggtgcag tcgctggagg ctcacgggct ctctgccgaa gctgtgcagg
    11101 gaatctgtat tacccacacc cacaccgacc atatcaaggg actgcgggta tttctgaacc
    11161 agcaccccgt accggtttac ggaacggcag aaacgctggc acagttgcgg gtgctggaca
    11221 aatttcccca gaacgccgac cttcgggaga ttcttcctgc ggaaaccata cataccgaca
    11281 ccgcagccgt aactgcattt gccaccatgc acgactcccc cggcagctgc ggctatcgct
    11341 ttgaactgtc agacgggcag acctgtgccg tgtgcactga tctgggctgt gtgacaccgg
    11401 aagtgaaaaa gggcgtatac ggagccgatc tggtgcttct ggaggcaaac tacgatccga
    11461 gcatgctgcg aaacggtccc tatccggaaa tgctacagga acgtattcgc ggaaagtacg
    11521 ggcatctctc caacggggac agtgccggta tggcagaggc tctcattgca catggcacca
    11581 cacggctgat tctggggcat ctcagcgaaa acaacaacac catgccgctg gcacagcaga
    11641 cggtactgga gcatttgcag gaacagggca tgcggcgaaa tcaggacttc ctgttgcagg
    11701 tttcctcgcc ggaaggattg aaacaggcgg tgatcttctg atgcagacga tacaaatcat
    11761 agcactggga aagtgcaagg aaagctacct gcgggaagcc tgcaaagaat acgaaaagcg
    11821 gctgtcccgt ttctgccagt tgcaggtgac agaactggag ccggttcccc taccccagaa
    11881 cccctcggaa aaggaaattg cggcggctct ggaaaaagaa ggggcagaac tgctgaaacg
    11941 taccaagggc ttttgcattg ccatgtgcat cgagggcaaa cagctggaca gcaccggact
    12001 ggcgaagaag ctggaacagg cggcacaggg cggcgagaac ggtgccgtca catttctgat
    12061 cggcagttcc tacggactgg ctgatgcggt aaagcagcgg gcaaatttgc ggctgtccat
    12121 gtccgccatg acgttccccc accagctagc acgggtgatg ctgctggaac aaatctaccg
    12181 gagctatcag atcttatccg gcaccaagta tcataaatag gagtgatttt tcatgcagaa
    12241 agcaatgacc atcgcctatg cagtaggcaa aaatctctat ctgaatatta ccaaccgctg
    12301 tccctgtgcc tgtacgtttt gtatccgcac catgtgtgac ggggcatacg gttccgaccc
    12361 gctgtggctg gatcatgaac cgtctatgga ggagattcgg gcagcactgg acaaagagga
    12421 cttgtcccat tatcaggaag tggtattctg cggcttcggc gaacctaccg aacggctgga
    12481 aaccctgtgc gaaaccgcaa agctgctgaa agcccgcggt gtcaagacca ttcgcatcaa
    12541 caccaacgga ctgtctgacc tgatccacgg cagaaagaca gcggcagacc tgaaaggact
    12601 ggtggacatt gtatccgtca gcctgaacgc cggcacagaa gcggaatacc tgaaagtcac
    12661 acgtccgaaa tacggtgcgg cagcctatcc ggcaatgcag cagtttgcac tggactgcaa
    12721 gcagtacgtg ccgcaggtga tgttcagcgt ggtggacatt ctgccgaagc cggaactgga
    12781 agcagcacag cagctggcag accgactggg aatccacctg cgaatccgcc agtttgacga
    12841 tgcagcgacc cgccggaacg gttaagagaa catttatcat ttacggaaac actgaataaa
    12901 gaggcagtac cagatctggt gctgccttta ttattttctg cacacctgaa acattttctg
    12961 aaaaccggtg tatatatcag tgaacgttca aaaacgaggt gataacatgg aaaccgattg
    13021 gattcgccgc ctgaaacagg acgacgaaac cgcactctgc gagatcatca gaaaatacac
    13081 cggctatgtc tataccattc tccggaactt ttcccacagc accctgccgc aggaggactt
    13141 ggaggaactg acagcagatg tgttcctgcg gctgtggaac agccggaacc gtctgcggga
    13201 cggcagcgac cttgccccgt atctggcggc gatcgcacga aatggtgtga aaaaccgctt
    13261 tcgcatgctg ggaaaacagc cgccggtgcg gcagtcctac gaagatctca cactgtccga
    13321 tgccaaagat ctctgcggcg atgcagaaat ggtggagatg atcgcctgtc ttctccgtgc
    13381 actggacagc ctgtgcgaac cggatcgttc tctgatgata cggttttatt tctatggaga
    13441 aaaaacctct gcacttgcaa aggcatatca catgacagat gccgcagtac ggctccggct
    13501 gcaccgcagc agaggaaaac tccggaagct gatgacagaa aggggttttg accatgtcga
    13561 atgaaaccaa ctggctgaaa gacttaccgc cattccagcc tgacgaagac ccccagcgtg
    13621 ccgaacgcgt gcaaacgcgt gtgctgcaaa agctggacga acctgtagaa agtgaggaaa
    13681 tcgctatgaa accgaaaatg acattccacc gtatccgccc tctgatcttt gccgccgctg
    13741 ccgtggctgt gggagccgtg tccctgatta ccgcaaatgc cgccaccggc ggagaactgt
    13801 tctcgaagct ttccatttac ctcaacggcg agaagcagga ctatgatgtg gaactggtca
    13861 gccaggacga cagccagctt acctacaaaa tcaaagacga aaacggcaac gaatacacct
    13921 atcaggagaa cagcaattct gtagagattc aggctgacgg cgatgttgcc gacggcgaag
    13981 tagaggtaga agtcagcggc aacgcgtccg acctgccggc agctgataca gcagaataac
    14041 aacaccggac acactttaag gaaacaacaa aaaaaccgga ctgcaccaca cagtccggtt
    14101 tttcttgatt gagaagtttt agggcagtat cgcacaaaga ttgtgcggta ttgccttacg
    14161 cattttgctg catgcccgtg tacagcaagt agtatctgcc cttttccgcc atgagttttt
    14221 catgactgcc ccgttcaata attctgccgt aatccaatac catgatcaca tcggaattct
    14281 gaatggtaga cagccggtgg gcgataacaa acacggttct gccgtgcatc agagcatcca
    14341 tacctgcctg tacaatgcgt tccgtgcggg tgtcaatgga agatgtcgcc tcgtccagaa
    14401 tcataaccgg cgggtctgca acggcggctc tggcaatgga gagcagctgc cgctgtccct
    14461 gtgacaagcc ggagccatca ccggacaata ccgtctgata gccgttcggc agcagcatga
    14521 taaagtgatg ggcgttggca cgctttgccg ccgcgatgca ttcttcatct gtcgcgtcca
    14581 gtctgccgta ccggatattg tccatgaccg taccggtaaa cagatttacg tcctgcaata
    14641 cgatacccag cgaacgccgc aggtctgcct ttttgatttt gttgatgttg atgccgtcgt
    14701 agcgaatctt gccgtctgcc agatcgtaga accggttgat caggttcgtg atcgtggtct
    14761 ttccggcacc ggtcgccccg acaaacgcca ccttttcgcc gggcttggcg tacaggctca
    14821 catcatgcag cacgattttt tccggcacat aaccgaagtc cacgtcaaag aactgcacgt
    14881 cccctgccat acgggtatag gtgaccgtgc cgtccccgtg agggtgcttc cacgcccaca
    14941 cgccggtgcg gtggtcggtt tctgtcagca cgccgtcctg ctcctcggca ttgaccagtg
    15001 tgacatagcc ctcatcggtt tccggctcct cgtccagcag ttcgaagatg cgtcctgctc
    15061 ctgccagtgc catgacgatc gcactggcct gctgggagat ctggctgatc ggcatggtga
    15121 agctgcggct caattgcagg aacgcggcaa ttgcacccag tgtcagaccg cctacgccgt
    15181 ggaacgccag tgcaccgccc agcattgcca gcagcacata ctggacatag cccagattgt
    15241 tcatgatcgg catgaggata ttggcgaagc tgttcgccca tgcggcgttc tggaacaatt
    15301 cgtcgttgtg cttgtcgaag tccgccttgg tctgttcctc gtggcagaac accttgatga
    15361 ctttctgtcc gtgaatcatt tcctcgatat agccgtttac cgcaccaatg gagttctgct
    15421 gcttcacaaa gaaagcaccg cttttgccgc ctacaaactt ggtaacaagc atcatcaatg
    15481 ccacaaacgc cagcacaaac agcgtcagcc agatactcag ccgcaccatt gccacgaaca
    15541 ctgcaatgac tgtgacgatc gaagaaaaga tctgcgggat actctgtgcc agcatctgcc
    15601 ggagcgtatc ggtatcattg gtgtacaggc tcattacatc gccgctggcg tgggtgtcga
    15661 aaaagcgaat gggcagtgtc tgcatatggg agaacatctc gtcccgaatg gttttcagaa
    15721 tgccctgtgc cacaacgacc atgagccggt tatagaacag cgtacccaga atgccggcgg
    15781 catacagcac gcccagcttt gccagtgccc ctgccagtcc ggaaaagtca cggctaccgt
    15841 tcagcagcat gggagaaata tagtcatcga tcagcgtttg caggaacgta gaacctgcca
    15901 cgcctgccac agcactgacc agaatgcaga caaacaccag tatcagctgc atccagtacc
    15961 gtttcatata cgagagcagc cggacgaagc tttttccggc acccttagct gctcccggca
    16021 ccatcacacc gcggggcggc ggtccctgtc gtctttcact cattgcggta cacttccttt
    16081 ctgctgtgat tcatatactt cccgataaat ggcgttattt ttcaggagct cttcatgggt
    16141 accgataccg tctatcctgc cctcgtccag cacaatgatc tgatccgcgt ccatcagaga
    16201 agtaacacgc tgggcaatga taaacttagt ggtatcgggg atttcgtccc gcagtgcctg
    16261 ccggatacaa gcgtccgtgt gggtgtccac ggcactggta gagtcgtcca gaatcaggat
    16321 cttcggcttt ttcagcagtg cccgtgcaat gcaaagccgc tgtttctgtc cgccggaaac
    16381 attggaaccg ccctgttcaa tataggtgtc atacccctcc ggaaaagtct ggataaattc
    16441 gtctgcatgg gcaagccggc agacacgttc catttcctcg tcggtggcat caggatcacc
    16501 ccagcggaga ttctccttga tcgtgccgga aaacagcacg ttcttttgca gcaccattgc
    16561 cacttcctgc cggagcgttt ccacgtcata ccggcgaacg tcgataccgc ctacctgcac
    16621 tgtgcccgct gtggcatcat acagacgggg aatcagctgc accagcgaac tcttggcact
    16681 gccggttccg cccagaatgc ctacggtcat gccagccgga atgtgcagat ccacgtcttc
    16741 cagacagcat ttatcttccc gcttgctgta actgaagctg acatggttga agtccacgct
    16801 gccgtccggc accgcggtca cggcatctgc cggactggta atgtcactct cctcgtccag
    16861 cacttccaca atacgctcgc aggaggcacg ggacatggtg atcataacga acaccataga
    16921 cagcatcatc aggcaggaca gaatctgaat ggcataggtc atcaggctca tcagttcgcc
    16981 agtggtcagt gtccggctga cgatcattct ggcaccgaac cacgatacca gaatcagaca
    17041 gacgtacatg cagaactgca tcagcggcat gttaaatgcc agccgctttt ctgccctgga
    17101 aaagtcctga taaatgttct ccgaaatgcc ggaaaacttc tggatctcgt gttcttcccg
    17161 cacaaaggat ttcaccacgc gaatcccgtg caggttctcc tgtgtcacgt tgttcagctt
    17221 gtcataggtt ttgaacaccc gttcaaaaat ggggtgcacc cgtgtcataa tgaagtacag
    17281 ccccacaccc agcaccggaa tggctgccag atagatcaga gccagtttcg cactcaggtg
    17341 aaacgccatt gccatggaga agatcagcat caccggcgaa cgcagtgcca cacgaattaa
    17401 catctgatag gcgttctgca cgttggtcac gtctgtggtc atacgggtga caaggctggc
    17461 ggctgaaaac ttatcgatat tggaaaacga gtagccctgt acgcggtaga acatatcgtg
    17521 ccgcagattc cgtgcaaaac cggcagaggc aaatgccgca aaatgccccg ccagtgcccc
    17581 gaaaatcagg gagagcacac agcacaccag caagatccag ccatagtgca tcaccgcgtc
    17641 catgccgccg ctgccgtcaa tgccgtcgtc gatcagcttc gccatgagaa tgggaatcac
    17701 cacttccatg acgacttcca gcaccacaaa cagcggtgac agcagggaaa acagtttgaa
    17761 ttcccgaatc gactgcatca attttcgaat cattcgcaat ctcctccttt acagatagaa
    17821 atgccctgct ttgcaaggtc ttcccgcaga ttttcctgca ttttctttgc cacgcggaga
    17881 aagacttcct gctcctcctc tgagattcca cgggcaagat ttgattcaaa cgccagaatg
    17941 tccgcttcga tctgccggtg cagatccctg gctttctgtg tcagcacgat tcgcttgagc
    18001 cgtgcgtcct gtgccacgct ctcccgccgg atcagcccgt ttttctccat cagttgcagc
    18061 gttaccgtca ccgaggaacg gctaatggaa aacgcctttt caatgtcccg ctggaacacg
    18121 tcccgttcct gattttcgta cagatagcga agcagatacc cgtgcatgcc ggtctgccgg
    18181 tctgcgtcct gatgtgccgc agagtacccc cgctgccgtg ccatcagctt ttccagccgg
    18241 tgcacctcta atcctaaatg ccgcatctta cacctcctga attgtttatc agaaaacagt
    18301 ttaatgatag actgtttagc ttggaactat tttagcacat tccccgaaaa tgtcaagagg
    18361 gattttgaga agtttacatt tttgtcaaac ttccgcccat ttgacaaaaa cgcatagaac
    18421 tggtatacta ttactattgc tattactatt aaggtatctt aagaaaagga ggcattcctt
    18481 tgcaaaaata tcggtattac ctgccgcccc agtcgaaact gcctttcgct gccggtattc
    18541 tctccggact gctctgtctg gacaacatct atacgctgat ttccatgccc cactacaccc
    18601 agtactggga ctactgggca acactgactg cggcactgct gctgtgtgtg ttctgcttcc
    18661 tgttccggca gcgtcgcaca gaactgacac tgattccggc ggcaattctg gcactgatcg
    18721 cctgcatcac ccccaacctc gttcactggc tggaggtggg catgttcttt ctgctgctgc
    18781 tgctctggct gctgcggctg cccaaatggg tgggaacact ctgccggctg gctggcattg
    18841 tcctgacggt catcggaagc ggagcgattc tgactcctat gacagaacga atctctatgc
    18901 tttcccgcac cggacgtgct actgcggaat tcgttgtgcc ctttgtcatt cgcagtctgg
    18961 gcggcgaact gctgatcctg ctgacgtttc tgctgttgat tttcgctatg cgtcctcatg
    19021 tgctgccccg ctggacagac gaaaacgacc tgtacgaccg catctgggaa tgacagatat
    19081 tcccatgaaa aacaaaaacc gtacctccgg cacagtctgc cgagggtacg gtttttttat
    19141 tgttaaggca ataccgcaca aagagcgtct ggcgactttg tgcggtattg ccttaatctg
    19201 ttttgccttt acgcaacgga attgacgaac atatcataga tgcagcgaat tgcctgctcg
    19261 aaatcggttt cctttacggc aacgattacg ttcagctcgc tggagccctg atcgatcata
    19321 cgaacgttga tatgtgcgtg tgccagtgcg gcaaagattc tcgcagccac accgcgggta
    19381 cggcacatgc cgcgacccac tacggcaatc agagcgatgc cgtcttcggt ttcgatgctg
    19441 tccggctgta ctgccttgat gatctcagtg cggatttctt cttccagacc gttcaggtga
    19501 gccgaatcca cgataacggt catggagtcg atgctggacg gcatatgttc cagcggaatc
    19561 tggtgttccg acagaacatt cagcacgtcg cggcagaagc cgatctggct gttcatcagg
    19621 tccttgtgga tattgatcga agtgaagtcc ttcttgccgg cgataccggt aatggtatac
    19681 gcagacagtt cctcatcggt gctgtccgga acgatcattg tgcccggttc ttccggagcg
    19741 ttggtgttgc ggatattgat tggaatgcct gcggaacgta ccggaaagat cgctgcttca
    19801 tgcagtaccg atgcacccat ataggacagc tcccgcagtt cagtataggt gatcgtgcga
    19861 atcactgccg gatcgtccac aatgcggggg tctgccacca agaaaccgga aacgtctgtc
    19921 cagttctcgt acagcgaagc ctttaccgca ccggctacga tagaaccggt cacgtcgctg
    19981 ccgccgcggg agaacgtctt gacatagccg tctgctccgc agccatagaa gcccggaact
    20041 acagcatgtt ccagtgtgct gaggcgttcc cgcagaacgg attcagtctt ttcatcgttc
    20101 aggctgccgt ccggctgaaa aaagatcacc tctgcggcat ctacaaaggg gaagcccaga
    20161 tatgcagcaa tgactttccc gttcaggtat tcgccgcggc ttgcggcata gtccttgccg
    20221 gcacgcttct tgaagtttgc tgcgatttcc tcgaactcat cgtccagaga aaaggtcatg
    20281 cccagaccgg tgataatatc gttataccgt tcccgaattg cctgcagggc atcggagaaa
    20341 tccagtccct gttctgccag atcatagcag gtgtacagca tatcggtcac cttgatgtcc
    20401 ttggagaacc gcttgccggg ggcagacggt accacatacc gccgctgcgg atctgccttg
    20461 atgattgctg ctgccttctg aatctgcttt gcgtccgcca gactgctgcc gccgaacttt
    20521 actactttcg ttgccatatg tttcactctg aaagaaaacc cgaagatctc ctttcaactc
    20581 cttatctgca tttatttgcg gtcaatctca aaaaccgccg gattgcactg cattcgcctt
    20641 atacttcgcc ttatacagtg tcactatatt agtatattgg tttttcgaca aaaaatcaat
    20701 agggaaatgc gacaaatttt ccttgtttta ttttgtgttt ttggtaaata cgcttgtatt
    20761 tatagggcgg acagattttt cttcctgtgg tatcgttcac ataacggcaa tttccataaa
    20821 tacgacgtac acaacttgtg cagattgtat atttcattct atgaaaactg gggataatcc
    20881 gaatccatag gttttgcatt attgattcag cccaaataaa tattggacgt accaattcca
    20941 cttgtggtat actaagtgag gcgaatgaca ataccattcg tccgcacaac gacacgattc
    21001 tacaaacatc aagggcaata ccgcacaaga aaaatggagg acaacatcat gggtggaatt
    21061 ctggaaacgg caatgctgat ctgcttcggc tgttcatggc cgatcaatct ggtgaaaaat
    21121 tacaagtgcc gcagtgccaa aggcatgagc ctgcccttta ttctcctgct gatcgtcggc
    21181 tatgtggcag gcatctgtgc caagttcatc atcggtgtca acgtacacaa cgcctatgtg
    21241 ttagtcgtat acctgctgaa tctggcaatg gtgacagcga acctgttcgt ctatttccgg
    21301 aaccgcggac tggaccgcaa ggcagaactc tgcgtggaac tggacgcgga cgatccggca
    21361 atggagctgg tagcagcttc tgttgcagac aaggtacagc agcgttctaa gagccgcggt
    21421 gctgtcctcc gccccaaggc tgccggcaat gccggattta tcgactatat ccagtggggc
    21481 aagacctttg aagaatgaca attctctccc ctgaacggca gtgctgccta cctctttccg
    21541 gcagtgctgt tcacaaagca tggattctgc aaaacgtgtg tgcttatctt caggcatcct
    21601 tgcagaaaca tgctccacat ataatcccac agaacaagga ctgggctgta ttttttacag
    21661 catggtcctt gttctgtttt ccatggaatc tgttgtattc tttgtacata aaattgtatc
    21721 atttgtgcat tgacatcgtt atttcgaaca gctataataa aggtatgctg taaaggaagc
    21781 actgaataaa gcttgtgcgt aagatgcgtc gtcagatttt tcggcagatt gcctagaaat
    21841 tccgcaggca tacttgatgt atggcaaggg atttctgggc aatatgacgg aaaatctgca
    21901 cgcagattgc g
//
LOCUS       DAWBGR010000017        20659 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_892_length_20659_cov_4.821879, whole genome shotgun sequence.
ACCESSION   DAWBGR010000017 DAWBGR010000000
VERSION     DAWBGR010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 20659)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20659)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20659
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_892_length_20659_cov_4.821879"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    17783..17792
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4700 a         5317 c         5490 g         5142 t
ORIGIN      
        1 gcaagcagat ggcgtgcaaa gtcgccagac gctctttgtg cggtattgcc ttagcaaaaa
       61 aggcaccgca gccatgcagc cgcagtgcct tttttatttg tgaaactcgt ttagaataac
      121 gatttcatta tgcgttcatg ctggagtttt ccggcaggcc cttcagaatg tgaacgagga
      181 acttcagcag agctgttgcg tcgttgccgt cgatttcacc gttctgatcg tagcagtcac
      241 cattcagcat ctgctgtgcg ttgaaggtta caacatttgc agccttcttg ttcagcagaa
      301 ctgcgtctgt aacgtctaca gcgccgtcca gattggtatc accaagcagc agctgacccg
      361 gagtgatgga tgcagcagtt acgctggtgg tggtagcatt gctgtcagtt gtggaagcgt
      421 tgcttgcatc tgtagtagca tcgcttgcat cagtggaagc tgcggtagta gcagcggttg
      481 ttgtagttgt atcctttgtg tcggtagaag aagtagctgt agtagtagaa gatgcatcac
      541 gttcgatgta cggagcttcg tcatccagga aggaagcaac ccagcacagc ggagagttcc
      601 agttgatggt gcactcgttt acagaccatg cttctacgtt atccagatag cacttcatcg
      661 gagcgataga acctaccttg tagccctgac cctgaaccat cgggtcttcc atgttggagt
      721 tcggaccacc ggacagaacg ccgtacggag catacgggaa gtcgtcgctg ttcagctgac
      781 cagaccacca tctgtggtgc gggtaagtgg tagagtgttc accgtaaccg gttacataac
      841 cctgttccag cgggttgcga cccatcaggt agtccattga agtggtaaca ccgtcaatgt
      901 actttacttc cttagatgcg tcatatgcca gagccagtgc catagagttg ttgattacca
      961 tggagttgga accccattcg taaccgccac tcagagtctt ggtttcagaa tcgcctgtac
     1021 cggaagcagc attgtacttc catacagtgg tatcatagtc atgacctaca tacgggatac
     1081 catatgcaga tttgtcctgt acttccagaa tggagtcagc tgccttcaga acttctgcct
     1141 tcagggtgtt tacttcatcc tgagtcagca ggcccttatc cagcatagcc tgagaattta
     1201 catacaggga gatagaacca acggagttca gagtacccca tgtgaacagg gagaatgaac
     1261 cgttgttttc accaccaacc agagttgtgg agatttccag agcctttgcg ttgtcagtac
     1321 cataagcgtt ggtaccatac ttcatcagtt catcataata ggtcttgtcg cctgttgtga
     1381 tgtacagttc gcaagctgcc cagtagaatt cgtcgctgac ttcggtgtca ccgtacgggc
     1441 caccgccctt gttctgatcc agcggagcat acatgaggtc agcagagata ccgccttcac
     1501 ccttggtgtc gccaccccag ccagagaact tcttgaagtt cttctgtgct gctgcatagg
     1561 tcttgattgc tgcatcttcc atggtctttg cataagctgc atcgtaatcc ttgaacagac
     1621 gagctgcctg tgcaaatgtt gcagaagcgt tcagagttgc tgcataggta accggcttaa
     1681 cgattcgcat cggagccttg ttttccttgt cgccttcgtt cttgtcatac ggagcaacag
     1741 ccagaccggt ccacttgtag tcgtgagcct tgtggtatac cataccatcg tcacgcatca
     1801 tttcgaggaa gaagtccagt tctaccttgc actcgtccag aatatccgga gtaccgtttc
     1861 ctgcttccgg aatagacata acggtggagt tatcgccgaa cttatcagcc ttgcctacca
     1921 tgagggatct ctcatacatg ttcatcagag tccacagaga gataccgccg tttacaacgt
     1981 acttaccata gtcaccagcg tcgaaccaac cgccagctac gttaatggac tggctgtagg
     2041 acggcttgga ggtgtagatg taaacccact tgtctgcgat gtaagcgtta tcagtcggcc
     2101 agtgctgaga atccttacga gccagagtct tcttgctgcc ttcctggctc tgcggagacg
     2161 gaatgtactt gtcttcagta tccattgcac ggttctgata gaagtagttc agtgcatagg
     2221 tcagcatgct cttgtcgtgg tgcttttcat acaggttctt gtcgatgtca aaactcttag
     2281 aagtcttgcc ggcaacagtc agggtgtagc ccttgccttc tgtagtgaaa tctgtgaagt
     2341 cgatctgctg ggtgtaatcc caggcaccag actcatcata cttggtgttg ccgtcggtag
     2401 tgccgctgta aacaacgctg ccgcttgcgt ccttgatctc gtagtcatac ttggtagtat
     2461 caccttcgga cagaacaaca gttgcaacct tcttaccatt cggatagtaa ccaacctggt
     2521 ttacttcaac gccggtcggc acataagcag cttctgcctg atagtcagat gcatcatcgg
     2581 tcatatcgat gagtgccagg ttgtcgaacc ggatgatggt atccttcggg aagcagtcca
     2641 tcttcgcgta gttaccctga ccgcccatgt ggaaggtcca ttctgcggta cccttaacgg
     2701 tttcagtagc ctggaattca taagcaactg tggtccactg gtttgccgga tattccttgt
     2761 tgtaccaagc atcccagccc tgaccgtagt ctaccttgtt accggtcttg gaagcactct
     2821 tcagcttttc ttccagctgt tcctgtgtca gaccttcttc gtaagacagg gacagttcgt
     2881 caccgttgct gtgccacagt tcctgatcat ctttggacat gttgcccagc tttgggtaca
     2941 tgtgaccgct gttagacggg tttacgctgt aggtaatacg ataggtgtga ccgctttcaa
     3001 tggtcagatt tctgtgacgg aactggcagt cccaacggct ttcaccgccg tttgcagtac
     3061 caccgatgtt ttccagcttg atcgcataga taccgtcggt gatgtcgaac ttcatgacag
     3121 cagttgcatt ttcacaaatg tgccaaggca agccctttcc gttttcgaag gtaccttcgc
     3181 ccagctcctc gcctgcgaac gctgtcatag acagtgcagt cagacctgta gcggtagcag
     3241 aaactgccat cactgcagca gaagcgaagg caagtgcctt cttgccgagt tttttcttca
     3301 gcataaatat cctccttgaa aattagagat tttttgatgt tggaacaagg gtacgcatgt
     3361 tccactttca acgattgttt tcgccgaaca cgccggcaga tatacgccgc accggatagg
     3421 attcagacgc aactatccct tacagggcat atctttctcg tttcatttat tatagcacat
     3481 gaaaaattca ttgtaaagta gattttcaaa tttcagtaaa acatgctaaa cagtttccat
     3541 gctttttgtg caacttgcca tatgtaacga caggtttttc ggaaaaaaca gaaaagaaaa
     3601 aggatacagg caaaatttgc ccgtatcctt tttgtgaaaa ttgccgattt ttgacagaga
     3661 ttagccctca gccggcagag tccttacgat cgaaacgagg aactggagca gtaccgtcga
     3721 atctgcctga ccaacaccgt catcggtgca gcagtcagca tttgcatact gctggtcgtt
     3781 caggattacc acgccggcaa ccttcttgtt cagcagaact gcgtctgtga tgtccacacg
     3841 gccgtccaga tttacgtcac cgtacaggct tgcctttacg ctcggcttcg ggccgttggt
     3901 agcggtggtt gtctgcttgg tggtagttgt ggtggaagcg gtggtcttgt tggtagttgc
     3961 cttggttgta gatacagtgg tagtgatctg accgctgccg ttgtaagcgt ctgtgtaaac
     4021 tgtaatagaa tcgatagtga acttgttgca gtcgatccac catacgccga acttcagttc
     4081 gccgccgtac tgggtctgaa tgatcgcaga agttgcagaa tccacatccc atacgatgct
     4141 gccggtcttt ccgctgatgg tctgttccat atcctcagac tgtgtccagt aatccggagc
     4201 cacgctggta gaagaaccga atgcaccctg ccacttgccg atctgcttct tgctggtgga
     4261 gaggttgatc tcaaccttgg tgaccttttc gcctgccgga acgcccagat cggaccactt
     4321 ccagcccaac atcttatcgc ctgccggcag ttcatcataa acaattgcct tattaatatc
     4381 cttggtatac ttaccgccgc tggaaggctt tgcagtagta gtgcccggtg ttacgttgcc
     4441 gtagtgcaga acgaccttgt cgatcttgac actgcttgca tccggataga agaccattgc
     4501 cttgacagtc tgacccacgt tggacgggat cacatagtct gcggtgcact gaccgttcga
     4561 accaacattg acctcaaagt cagtctgttc ccaggaacca gtccatgtgc cgaatgcacc
     4621 gctggagttg gtgtccttgg tagaaatggt gtagtacagc gttgcagact ttgcaccgtt
     4681 gggagcaaac tctgcgtaca gctgcttgtc gcctgcggta ccagtctgtg tgccggttgt
     4741 aatgtttgca gtcttgtcat cgccgcttgc cgcagcagta gtagcagttg tagtcgggtt
     4801 atgcttggta gtagttgcat tggttgtcgg gttgctgctg cttccttcgt ccagaacgac
     4861 cttgtcgatc ttgacgctgc ttgcgtccgg atagaagacc attgccttga cagtctgacc
     4921 tacattggac ggaatgttat aagttgcctt gcactgacca tttgcaccaa cagtaacctc
     4981 aaagtcagtc tgttcccagg agccggtcca tgtgccgaat gcaccgctgg agttggtgtc
     5041 cttggtggaa atggtgtaat acagagttgc agtctttgca ccattgggag caaattctgc
     5101 gtacagctgc ttgtcacctg cggtaccagt ctgtgtgccg gtggtgatat tggcattctt
     5161 gccgccgccg gaagtatttc cactggtagc aacagttgtg gtgaccggtg tgttgctttc
     5221 gtccagaacg accttttcga tctcaacgct gcttgcgtcc ggatagaaga tcattgcctt
     5281 gacagttgca ccaacgttgg acggaatgtt gtaggatacc ttgcactcac cgtttgcacc
     5341 aacggtaaca tcaaagtctt cctgctccca ggagccgatc catgtgccaa atgcaccgct
     5401 ggagttggtg tcactggtct tgaccttgta gtacagagtc gcagtctttg caccgttggg
     5461 agcaaattct gcatacatct gcttgtcacc tgcggtgccg gtctgtgtgc cggtggtgat
     5521 gtttgcagtc ttaccgccgg acggagcggc agtggtaatt gtagtggttg tgatattctg
     5581 cttggtagta gcctgtgttg tggttgtagt cgccggctta ttggtggtgg tagttgttgc
     5641 cttggtggtg gtaacaatgc tgccgctgac accttcaatg ctggagtcta cagaaccggt
     5701 tttgtaaata gaatacagac ctgctgctgc accgaccaga ccagcgttgt agtccagagc
     5761 cacttcgttt gcctgatagt cgtttacaga gtcagtataa gtgaagttgg agtctgccgg
     5821 accgccgacc agagcaccga ccagcgtgtg gccattgctg ctgtagtcgg ttctgccctt
     5881 catttcgtca aaactctgat agccggatgc agctctgtgg tgcggatact ttgcagaatt
     5941 gctgtccatg ccgacaacga aattcacatg cttcgggttg ttgcccagga tcatgcccat
     6001 ctgtgccttg caccagctgg aataatccat gccgctcttt gcggaatatt tcgtggtagc
     6061 cagtgctgcc atctgcagtg cagtgttata acgtgcactg ccccagtcgt tttcgcagta
     6121 atatgtgctt tcgctggtgc acttgccgtt cagatatttg gtgacctttg cccagtccat
     6181 tgcatcgccg gtgatctcac tctggaggat cgcagcaccc aggctgacat tattccagca
     6241 catggcggag tatacgcccc acttgcatcc ggactgacca gacttaccag cattggagtc
     6301 gttcatgaac ttgtccagga acgagctgta ggtgctgtca ttggttgcca ggtacagcca
     6361 gccagctgcc catgcctggt catcataata gtcataagag ccgtagaagt ttgctgcctc
     6421 cgggcagttt gccttattgg tcttgatgga atagttgtac agtgcctttg catacttcag
     6481 gtcttctgcg ttgccgaagt tgatgtagtt gacagccagt gctgctgcat attctgcggc
     6541 gatgtcgctg gcatcatcag aagtccagta agcagtacgc agactctggt cattctggga
     6601 ctccggagag caccagacat catggtcagc ctgtcctacg cctacctgat agcagaagct
     6661 ggttacggta tcgccgctca gctttgtgct tgccttgaag aagtcacaga agcgatcggt
     6721 cagtgcctgc aaatgagccg tctgtcccag tgcatcatag ctgtccttga attcataata
     6781 gccccagccc agagtagaag cggtgtagcc agccggcaga ccaaacttta catggtcgcc
     6841 tgcatcgtgg aagccgccgt ccacttcatc gctgccgtgg cagttatctc tccagtcaaa
     6901 ctgggaggca cttcccacat cagaaccgca catattgccg tcgtaaaaat acatggagta
     6961 ctgcagcagc tttgcgtagt tgtcataaga cgcagcagat gccgccatgc ttcccagcgt
     7021 tgtcagtccg gacaacgctg tagttccggt cagtaacgtc gcagcactgg cagcacacgc
     7081 cagtacccgc ttaccgagcc caagtttctt cataaaaaag ctcctttcgt agttcgcatt
     7141 ttttctgcac cgttctgtcc caacttcagg acaattctta aaaagtgcag acatgcccga
     7201 aatttattct atcatacctc acaaaaaaaa tcaacagttt tttcctttca tgcgagaaat
     7261 ctatgaaaac tgcccaaacg gcatctggat tttttagcca taatgtcaat ttgctgccga
     7321 aatcatgctg attttgcaaa tgcatacacg ctccaaaagt gctgcatcgg tcagatccgc
     7381 gtttccacgt cacgcagctg ttcctgtaac tgtttccagt ccggcagttc tgcggcgatc
     7441 tccgcataaa atgccggcga atgatccgga tggagaaaat gggaaaactc atgcatcacc
     7501 acgtactcca tgcaccgcac cggtgccgcc agcagccgca gattaaacgt cagcacgccg
     7561 cgggtgggac ggcagttgcc ccactgggaa accatatgcc ggaaccggat ctcaggaaat
     7621 gccacgcctt tcgcttcaaa cacgggataa actgcacggc agagattccg cactgccttt
     7681 tcgcaggcgt tttcccacca gctatcgaat acctgtttcc gaacctgttc gttttccggc
     7741 tgtttcacca gcagccggat tgtttccccc tgcaccagta cgctttcccg actgccgcgt
     7801 tccaccgcca gccgacagga tttccccaga tacagcactg gttctccggc ggtatagtgc
     7861 accggatact gtttcggctt ttctgcggca tcggcgtatt ggtgcagggt ctggagaatt
     7921 ttcgcggcat gctgctgtaa gatctgttcc acccgttctg acggtacatg catcggtgca
     7981 gaaaccagca gaccagtttc cggacgaagc cgcatgttga tatttttgac agctttccgt
     8041 tccagcgtgt aggaaatggt ttcgccggca agagtgatgt tccgctgttc tttcatgggt
     8101 cggtgtcctc gctttcggtg agatgcattt ttgcagcact tgacagatcc tgcaatccga
     8161 tgtataatgt atataaatta gggaatgatg ttctcccttg ggattccaaa accgcttgag
     8221 tgctgatgac atctgctttt tagggtctgt ttcatatttt ttatctgccg aaaagacgtg
     8281 cagaaaaaga tatgaaacag gtttctaggc agatattgtc agctttttct tttttcggag
     8341 gtattgtcta tgcaaaaaat caaactgcaa cttattttcc ccactctgct gctctgtgtg
     8401 ctggcggcag ctgccggctg tatcaccggt gcactcaccg ccggattcgg gcgtgtgctg
     8461 ctgtggatcg gtacattccg gacagagcac ctgatctcgt gtgttccctt tctgcccatt
     8521 gccggtgtct gcatggtatg gatataccag aaatacggca gagaaagcag tcagggcatg
     8581 agcctgatct ttgatgctgc ggacggcact gcaaaacgca ttcccctccg gctgatcccg
     8641 cttctgatcg gcagcacgtg gctgacacat ctctgcggcg gcagtgccgg acgagagggt
     8701 gtcgctgtac agctgggagc tgcggtttcc cacaacatcg gcagccggat tccgtggttc
     8761 cggagcattc ccgatgccgt tcccattctg accattgccg gaatggctgc cggtttcagc
     8821 gggctgtttc acgcaccgct ggcggcagtc tgctttgcac tggaagtgct ggcaatcggg
     8881 aaactggaat atcgtgccct gctgcctgcc gccattgccg cagtgacagc aaacgggatc
     8941 tccgtcgcac tggggctgga aacctttcag gtggcacttc ccgaagccgg cttcgcaccg
     9001 ttttccgtgc cggttctgct gctggggatt tcctccggca ttgtaggcgg catcttcacc
     9061 ctgctgctga aacaaggaaa aacctatgcc gccaagctgc tccccaaccc tgtgattcgc
     9121 attgccctct gcggttcagt actggcaatg tttctcctgc tctccggaca cggcagatac
     9181 accggactgg gcaccaatct gatcgccctc gcctgtgacg gcggaaccat ctactggttc
     9241 gactttctgt gcaagctgct gttcaccgtc tgcacccttg ccattggatt tcagggcggc
     9301 gaagtcacac cgctgtttgc aatcggtgcc tgccttggtg cagcacttgc cccgctgttc
     9361 ggtatctctc cggtgttcgc cgccgcactc tgctatgccg ccgttttcgg cagtgcttcc
     9421 aatacatggc tcgctccgat ctgcatcgga accgaagtgt tcggattcgc gtacctgccc
     9481 tatttcgccg ttgtctgcac cgtttcccgc ctttgcaacg gcaaccgttc catgtatggc
     9541 agacagaaac cggctccgct gtttttgcag gggcaaaaat aagacacgtt ttcaaaaagc
     9601 cgtacatgat cgctcatgta cggcttttaa ggaagcagat ttctcaaaga gggttgcggg
     9661 agagccgctt cagcagcgga atgcacacac caccgcacag atttcccaga attgccaccg
     9721 ggaaataaca cagtgcacga agcgggttcg ggcagccagc catgcagtaa tagaacgcat
     9781 ctgccacgca gtggttgaag ccggacagaa tgaataccat gatcggcatt accacaatga
     9841 acacgccgcc gatgaagttc ttcttttcgt tgcagcggcg ggatccttct acagcggaga
     9901 acatcagaat gccgcagaag aacgccagca caaaagagct aagcaagctg tcgtccaact
     9961 tggcggacat ggttgtctgt gcacgatcca tcagtgtcac gtccatgccg tttaccttgg
    10021 tttccgttgc gaaccgtgtc aggtgcacga tgccggcagt caggccggca ccgatcgcat
    10081 tcgccaaaaa ggtgaagaac gtttcgccca gatacgcctt gtcacggtcg aaaatgtatc
    10141 ccaccttgcc ggtgtacagt ccatactgga actgcacaat gcagaacagt cccagcgaga
    10201 acagaaatgc ccccagatag cggttttcac aggacatgct gacgacacag ccgatgccga
    10261 tcatcagccc ggacagcata ccgtccgcca gaaaggacag ccacttattt ttttgcttct
    10321 tttccatttt ttatttccta cactttctat actttaggaa acactgcata aagcttgtgc
    10381 gtaagatgcg tagtcagatt tttcagcaga ttgcatagaa attccgcagg cagactgtat
    10441 gtctggcaag ggatttctgg gcaatatgac ggaaaatctg caagcagatt gcgtacaaag
    10501 ccgtcaggcg ggctttattc agtgtttcct tagcccaaaa caacttcaat ttcgttcttg
    10561 tcagccagtg cgtcagccgg tacatagtcg ccgtccagtg ccttgccgtt cagtgtcagg
    10621 gaagccacgc cgctttgtac gtggttcggg ttcttgaccg tgatagacag ctgcttgccg
    10681 cggaacagtt tttccatggt gaagccgtcc catgctgccg gaatcgacgg gcagatgtgc
    10741 agaccgtcag cgtccggacg catgccgcag ataccctcta cgcagccaac cataacggtg
    10801 gaagcggtac cagtcagcca gtgtacgtga gaacgtcctg catacgggga agcggtagac
    10861 tcggtgaact gaccgtgtac atacggttcc agtacacgct gttctgcctt gtcgttcatg
    10921 gaagccggag aactttcctc gaagtagcgg aatgcatcgt caccgtgtcc cagcaggctc
    10981 tctgccagaa tcagccagcc ctgcgactgg gagaagatac cgccgttttc cttggtgtcc
    11041 ggattgaaga tgtgcatcag ggcaccgtcg aaatagtggt cgatatacgg cggatccagc
    11101 agctttgcac catacggggt gtccagaatg tctgctacag actgcattgc cttgtcgctc
    11161 tgttccttgg aagcaaagcc ggaaatgacg ctccagctct gggggttcag ccacatggaa
    11221 gcttccggat ccttcttggc accaactact acgccgtctt cgcggatacc gcggataaag
    11281 cggtcttcgt cccagcatgc agccagtgct tcgcccagag ccttctgagc cttgtccaga
    11341 taggaaacat actcgctgtc cttgcgttct gctgccagtt catgcatcat ggacattgca
    11401 tagtacagct ggaatgccac gaaagtggac tcgccctttt tgcccagacg caggcagtcg
    11461 ttccagtctg cgtgcagacc agccggcatg ttgtggtcgc ccagacgttc catggagaag
    11521 cggatcgcac gcttcaggtg gttgtaaaca gtatcttcgc cgccgtttgc atagacgatc
    11581 acttcgtcat agaatgcctt gttgccggat tcgcccacat acttgcgaat ggtggggaac
    11641 agccacagag catcgtctgc acggtaggac gggtgaccgg tttccttgac atattccgga
    11701 tcgtccggtg tgttttcatg accggcgttg tggtcgaact tgaccagcgg cagaccgccg
    11761 ccgttgtcta cctgtgcaga cagcataaag cggatctttt ctgcggcgag ttccggatcc
    11821 agatgaatga taccctgaat gtcctgtaca gtatcgcggt agccgtagcc gttccgcaga
    11881 ccgcagtata caaacgatgc ggcacgggac cagataaagg tgatgaagca ctgataagcg
    11941 ttccagacgt tgatcatgtt gttgaacgca tcagacggtg tgctgacctg gaaatggttc
    12001 agtttgctgt gccagtattc cttcagagcc tctacttcgc tgtcgacctt gccggcagct
    12061 tcataggaag cgaggatttc gtttgcctgt gcattgtctt tctgacccag tacgaagatc
    12121 agttccttgg attcgcccgg ctggagggtg atgtcactct ggagagcacc gcaggcattg
    12181 ctgttgtagt tcatctcacc ggtgcacttg ccctgctcta cagcgatcgg gttggaatag
    12241 gtgcggtacg gaccaatgaa gctgtccaga ttgccgttat agccggagat cggagcacct
    12301 accatgccga agaaacgctc tctgtggttg gaaccggtag cgtccttgcc gtcattttcg
    12361 ttgatgtgct ggaggatctt gttgccctca aagctggtac gggtgatgaa cagggtgtac
    12421 tggaggttga cctgatcctg ttcgtagttg ttgtcgttgg taaactctac gaaaccgaat
    12481 gtggacaggt ttctcggctt gtcgctgttg ttggtgatga ctgcacgcca tacctcataa
    12541 gttgccccga tgggtacata ataagtgact tcggaggaaa tgccggcata gtctgcggaa
    12601 attacggtgt aagcagtacc gtggcggcag acgctcttgt aggaatccag cggcttgcct
    12661 accggctgcc atgtagcaga ccagtaatcc ttggtgtcgt tatcccgcag gtaaatgtag
    12721 cgtccgggca gtgccatcat ggagttgaag cggaagcggc tgattctgcc gtttgcaccg
    12781 gacttgacaa agctgtatcc ggcagcgttg ttggagatca ttgcaccgta ttccggagaa
    12841 ccgaggtagt ttacccaggg tgccggcgta tccggtcggt cgatcacgta ttctttgttt
    12901 ttcaggtcaa aataaccata ctgcataggg agtatctcct ttacatctgt attttcaatg
    12961 tcaaatgacc atcaccgata aagacagtgt ggtcgtacat ttatcatacc ataccacaga
    13021 agattttgca agggatttgc aaaaaaaatt gcagtaaatg tggtatggct tctgtgggct
    13081 ttgcagattg tgcagtattg ccaaaaagtt ttcccgccgg cttgacagac aagcttttgt
    13141 gtgctataat gtgcttatta aaccagtaca attagtacaa aggaggggag cccgtggaaa
    13201 ttatcatcag cggcaacagc agcaagccca tttacgccca gatcacggag cagctgaaag
    13261 cccagatcat ggacggcacg ctgcatgccg gcgaatcgct gccgtctatg cgaagtctgg
    13321 cgaagtctct gcatgtcagc gtgattacgg tgcagcgggc gtatgaggaa ctacagcggg
    13381 acggatttct ggaaacgaca gtcgggcggg gcagctttgt ggctgcccag aatccggaga
    13441 tctatcggga agaacagcag cgaaaggcgg aggagcacct gctggcggcg gcggaaattg
    13501 cccgcgtcag cggcatttcg ctggaatcgc tggtgcaggc actgaccatt ttttatcggg
    13561 aggaagaata gccatgcaga atattttaga agtacgggat ctggggcgtt cctatgtgaa
    13621 tttccggctg gaacacatca gcttttccat tgccggtgga acgatcatgg gcttagtagg
    13681 cgaaaacgga gccggaaaaa gcactaccat tcggggcatt ctggggctga tccggcggga
    13741 gtgcggagag gtgctgttct gcggcgaacc gctgaacaca gacagcgttg ccatgaagga
    13801 gcagatcggc gtggtgtttg acagtctttg cttttcaccg gaactgacgg tggaaaaggc
    13861 ggggaaaatc tgccgcagca tctattccaa ctgggacgaa gcctattttc aggaactgtt
    13921 gcagcagttc tccctgctgc ccaagcagaa ggtcaaggaa ctgtcccgcg gcatgagcat
    13981 gaagctttcg ctggtgctgg catttgccca ccacccgaag ctgttgatcc tggacgagcc
    14041 taccagcgga ctggatcctg tggtgcggga cgacctgctg gatttgtttc tggaattcgt
    14101 gcaggacgag gagcatgcca ttctgatctc cacccacatc accagtgatc tggaaaaggt
    14161 ggcggattat gtgacatttc tccatgcggg aaagctgctg ctttccaagc ccaaggacga
    14221 actgctctgg cagtacggca ttctccgctg cggagaagca ttattccaga agctggaatc
    14281 gtcagaggtg ctggcgtggc ggaaaaagga ctattcctat gatgtgctcg tgccggatcg
    14341 ggaggcaatt ttgcggaagt atccggaggc tgtcatagat caggtcacaa tggacgagat
    14401 cctgttgttg tatgtgaaag gagtgcaggg taaatgaaag gactgctttt gaaagatttc
    14461 tataccatat ggaaaggcgg acgaattttc ctcattctgt acgtctttat ggcagctgtc
    14521 tccggcgtga atatccagaa cggctggtct tacatgggct tctggctggt gatgatggac
    14581 tcgcagacct caagcacttt ttcctatgac cattcctgca actggaaccg ctttgcactg
    14641 acaatgcccg tcacacgaaa agacgtggtg cggtcgaaat tcctgttgca gctgctgatt
    14701 gtatgcggca gtacactggc gtatctcgtg ctggcagccc ttgtcagtct ggtactgcgg
    14761 acgtttgaag tcaaggcatt ttgcaatatg ctgtttgccg catggctcgt gctctgggtc
    14821 gtgctgctca tgggcagcgt ttccacactg ctgctgttcc ggttcccgcc ggaatatgca
    14881 cggctgatct ccattttgat ctatgtgatt ccggcgtgtg ctttcatggg tgcgggcagc
    14941 gttttcatgc aggaaatgac agacaatctt gccgctctgc ctgcggaatt ttccgcaatg
    15001 ctgctgccgg cggcgattgc catgccggtg ctgtcgctgg tggtttccgg actgtgctat
    15061 gtctttagct gccggatctt ccagaagaaa gatctggcat aactccgtga tgcagcagca
    15121 tctccgcaga aaggactgtt attgtgaaac ctattgctca tattgaaaac gattttccca
    15181 ccaagttcgg cattccccgc cagagtggac tggtcaacag tctgcatgcc cgcattatta
    15241 tggagccgga ataccggaat cccgatgcgt ttcgggggct ggaggattac tcccacatct
    15301 ggctgatctg ggaattttca caggcggtgc ggcagaagtg gtcgcctacc gttcgcccgc
    15361 cccgtctggg cggcaatgtc cgcatgggcg tttttgcgac acgctcaccg tttcgtccca
    15421 atccgctggg gctgtcggtg gtgcagctgg aagaaattac attgcacggg gcgaatgctc
    15481 cctacctgac ggtttccggt gccgacctga tgcacggcac gcccatttac gacatcaagc
    15541 cgtacctgcc acacattgac agccaccccg aagccagggg cggttttgct ctgccggcag
    15601 cggctcatcg tctggaagtt cgctttccgg aagtctggct ggagaaagtg ccggaatccc
    15661 tgcgggcagg gctgaccgag gtgctggcac aagacccccg tccgtcctac cagcacgacg
    15721 ccgatcgggt gtatggcttc ccgttcgccg gtctggaagt ccgctttacc gtggacggcg
    15781 aagtgctgac ggtttgcgaa attctggaag cgacggtata aacttacgac atctgcacat
    15841 acttcaggca gtatcacata aaggcaatac cgcacaagct ttattcagtg tttccgtaga
    15901 aaaaatttcg tcggatactt gacaaggcga aggttctgtg gtacaatatc tttagttaga
    15961 gataactaac ttcaaaatga acatgcgtaa tggagaagga aacacggtat aaagcttgtg
    16021 tacacagccg aaaggcggga tttgtacagt gcttccgcga tacgtaaagg agtattctac
    16081 tatgagttat ttgcagatcg aaaaagttgt tggcagagag attctggatt ccagaggcaa
    16141 tccgactgta gaagcagaag tcacactggt tgacggtact gttgcaagag gtactgcccc
    16201 cagcggtgct tccaccggcg aattcgaagc actggaactg cgtgacggcg acaaggctcg
    16261 ctatctgggc aagggcgtga ccaaggctgt agaaaacatc aataccgtta tcaacgaaac
    16321 tatcactggt atggacgcat ctgatctgta cgcagtagac aaggcaatga tcgccgcaga
    16381 cggcaccaag gacaagtcca agctgggtgc caatgcgatt ctggcagttt ccattgccac
    16441 agcaagagcc gcagcaactg cactggacat tccgctgtat cgttttctgg gcggtgtatc
    16501 cggcactcgt ctgccggttc cgatgatgaa cattctgaac ggcggtgctc atgccaccaa
    16561 tacggtagac acacaggaat tcatgattat gccggtaggt gctcccagct ttaaagaagc
    16621 cctgcgttgg tgtgcagaag tgttccataa tctggcgaag atcctgaagt ccaaggggct
    16681 ggcaacttct gtcggcgacg agggcggctt tgctccgaac ctgtccagcg atgacgaaac
    16741 cattgaaacc attctggaag cggtaaaggc agcaggctat gagccgggca gggatttcat
    16801 gatcgcaatg gacgcggctt cttccgagtg gaaaagcgaa aagggcaagg gcttctatca
    16861 ccagccgaag tccggcagag attttacttc cgacgaactg attcagcact gggctgatct
    16921 ggtagagaag tatccgatca tctcgatcga agacgctctg gacgaagaag actgggaagg
    16981 ctggcagaag ctgacggcaa agctgggcgg caaggtacag ctggttggcg acgacctgtt
    17041 cgttaccaat actgaacgtc tgaagaaggg catcgaactg ggctgcggca actccattct
    17101 gatcaagctg aatcagatcg gttctgtttc tgaaacactg gaagcaatcc agatggctca
    17161 caatgcaggc tacactgcaa tttcttctca ccgttccggc gaaaccgcag atacgaccat
    17221 tgctgatctg gcagttgcac tgaatacctg ccagatcaag accggtgctc ccagccgttc
    17281 ggagcgtgtg gcaaagtaca accagctgct ccgcatcgaa gaagaactgg gcgacagtgc
    17341 cgtatatccg ggcatggcag cattccacgt caagtgattc taatgtctgt gcaacactga
    17401 aacgactgtt ttaggatttc ccttggaact cttggcaaat gaggctccct tgaaaagggt
    17461 gattccccat ggaatgggga aatgtcatga agtgacaaaa gggacggctc ggtaagagct
    17521 ggcaagccgc aggcttgact gagggattta aaactataag taaaagattt tagtagaatc
    17581 cctccaccag ctttgctggt ccccctccct tttcaaggga ggcttgttcc tgaaattgtt
    17641 gtaatgccat aataatccta tgcacgagtt ttgtacgcag ttgtctaata gacattcccc
    17701 acacaacatt tgctttccct tcagggtgag atttcacccg cttttagaac tctttcataa
    17761 ccttatggaa aaacggtacg cannnnnnnn nntaaaggtt ttccttttta ttttcaatag
    17821 catattatta ttttagaaag cgaggtcgat ttttatgaaa agaagaacaa ggataatttc
    17881 tgctgtttgt gcagctgcac tgttgggaag tgcaatgacg atgccggcag cagcataccc
    17941 catgcatatg aatggggaag ctgaagtgcc gctggcacag gtgctgcaga tgctggaaaa
    18001 tgacaagacg gcatatgtgg aaaagggcga aagcatgtac tatggcttga aagatcccac
    18061 accagtaggc atgtatgaca acatttttta tgcaacggcg gaacaagatg atttgattca
    18121 gctcaacgag tatgcatatc ggattcagct ggagcaggac gtggcagatg tgcgggaact
    18181 gacagaacag ctttgcggtc tgctgaccaa aaaggaaaat gcagaggagc cgactgccga
    18241 tctggcattg agtaccgaag aatcggaaac acgccagctg actatgaccg cagagggaag
    18301 cggagcatat acgctgcgat tcagcacatc ctcgtatcgt ctggacgagg aaaaactttg
    18361 tcagttcctc cagaccttgt ccggttttgt atcggtacag caggaagtct ggggcgtttc
    18421 tttaaagaaa aacaatgttg caccaatgtg gggtagtcaa gacagttctg gtatttgttg
    18481 tgttactctg aaagaggggg ctgttctgac accggaagat ctggatctgg actttgatgt
    18541 ggagatcgaa caaaatgaaa aaggcacggg tactataatt cccaagaaat ctcttgacat
    18601 gcagcagcgt tatcaattgc tgcggaaagt ttctctcatg atggaagccg gagagaaaat
    18661 cgatcaagta agtgttatct atacaatata tgaatctgcc ctcaatacag cggcagagtc
    18721 ggacggctcc atacagctaa tggggcgggg cgactgtgac ggcagcaagg aactcgacag
    18781 ttctgatgtt ttcgaactgt tacagcacac ggcaaatgtc ggtgccggaa acgagggtac
    18841 gctgaccggc aaggaactgc ttgcggctga cattgacggc aacggcagcg tagattctac
    18901 cgatctcttt gaactgctcc gctactgtgc agaaaagggt gccggcttgc agcccacctg
    18961 gacagaaatt ctgggctgat tgcagggctt cgcatgctga tacagacttt gaaatattgc
    19021 tgtaaataga aatattccca cggggtgctc cctgtgggaa tatttttttg ggcggtattg
    19081 cctttggtta tttttctcgt tgtgtcccga tctgtttccc cagaagcagc agccccagca
    19141 ggttcggcac agccataagc ccgttggcga tgtcggagag tgcccacacc gaccgcaggg
    19201 aaaccacagc ccctacgcct gccgccagac agaacaacac ccgatagggc agccgccacg
    19261 cttcaccgcc cagataccgc accgcctgtt ccccgcagca gcaccagccg atcagcgtac
    19321 agaatgcaaa cagtgcggtg atgcccgcca tcagttccgg tgcggcttgc ccgaaaacgc
    19381 ccgaaaatgc ggcggaaatg atgccgccgg cattgtctgc ctgcgtcatg gctccgctgg
    19441 tgagaactgc cagagccgtc agcgtacagc acaccagcgt atccaatgcc acctccacca
    19501 tgctccacat gccctgtagc tgggcatctt cggaactgcc gccggcgtgg acaagggcac
    19561 tgctccccag tccggcttcg ttggaaaata ccccgtgccg cagaccagtc tgcacagcac
    19621 gctgcaaaac agcaccggag attccgccgc ctacggcatg cagcccgaat gcttcgcgga
    19681 aaatctgccc gaatgctgcc ggcagtgcgg cggcgttccg gaagatcact cccagtgccg
    19741 ccagcagata caccgccgac agcaccggca tgagccattg aatgaccttg ccggtggagc
    19801 gtgtgccgcg gagcaaaata attcccagca gaagcgtgac accgattcct gtccacagcg
    19861 gcggcaggga gaacgcctct gcggcaaggg tggaaatggc actgctctgt gtcatgttgc
    19921 ccatgcccag cgaagcaccg atgcagcaaa gactgtaaaa tactgccaga acgggactgt
    19981 gcagcccgaa ccggagatac gccatagggc cgcctgcctt ggtgccgtcg gggagtgtgc
    20041 gggcataccg cagagccagt acattttcgc tgtaagtcag agccatgccc agcagggcgg
    20101 atacccacat ccagaaaatt gcaccggcac cgcccagaca gagagccgcc gccacgccca
    20161 gaatgtttcc cgtgcccatc gcggcggcaa gagcagtgga aaaggtctgg aactgggtaa
    20221 gggaagtgcc gcccttgccg gacttggtac gcaggctgcc gaacgtccgc cgcagaatgg
    20281 ttttccagcc ccagaactgg aagaaccgca gccggaaact caggtacagc cctgtcccca
    20341 gcagcaacac cagcagtcct gttccccaga tgtatgatgc tacgcgttcc agcataattt
    20401 ccctcctgac agaatatccg tagtttatca tgatctgtct gcacaaaacg gtttatataa
    20461 tttatataga gaaatcgccc ctgaaaggga agatgtcacg aagtgacaga ggggtattgc
    20521 ctatgaggaa taaaaaggat ttgtgtgcaa tctattcgca ttctcttgac gaaaatgtgt
    20581 tctcggggta taatagaaac agagcagatc gctctactag ggggtgttga cactataatg
    20641 atatgcgaat tttgtgagg
//
LOCUS       DAWBGR010000018        20629 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_895_length_20629_cov_3.921308, whole genome shotgun sequence.
ACCESSION   DAWBGR010000018 DAWBGR010000000
VERSION     DAWBGR010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 20629)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20629)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20629
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_895_length_20629_cov_3.921308"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4945 a         5071 c         5956 g         4657 t
ORIGIN      
        1 tctgacgaaa aatttgacga cgcatctgtc gcacaatctt tgtgcggtat tgccttaaga
       61 accttataaa tcacaattcc ggtaagtctg ccgtgaccgg tttgtccggc aggcttttcg
      121 gctgtcagga aagaggcgtt tcccatgaag aaatacgacc cgtttgaaaa tgtgcagacg
      181 gtaatggaac aggcggcgat actgggaaag atcgaccgca gaatgtttga gatcatcaag
      241 aatccccagc gggaaacgaa ggtgtatctt cccgtagaaa tggacgacgg cagcattcgg
      301 gtgttcgagg gctatcgtgt gcagcactcc aatatccgcg gcccgttcaa gggcggtatc
      361 cgttaccacc agaactgcga cctgaacgag atcaaggcac ttgccacatg gatgtccctg
      421 aaatgtgctg tggcgaatat tccttacggc ggtgcaaaag gcggcattca ggtggatccc
      481 agtaccttgt ccaagcggga actctgccgt ctgacacggc ggtataccta cgccattgaa
      541 ccgctgatcg gagcggacaa ggatattccc gcaccggacg taaacaccaa tgcccagacc
      601 atggcatggg tgctggatac ttacagcatg ctccatggaa aaccatgtcc gggcgtagtg
      661 accggaaagc ctgtggaact gggcggttcc agaggacgtg cttccgccac cggcagaggt
      721 gtggtcattg ccacccagct ggttctgaag accaacggcg tggattcact ggacggcgta
      781 aaagttgccg tgcagggtat gggcaatgtt ggagccaacg cggcacgaat tttctgtcac
      841 cgcaacgcct gcgttgtggc actcagcgat gtgtccggcg gcatttactg cgaatccgga
      901 ttagatgccg atgcggtttc cgcgtttctg gaagaggggc acctgctgaa agactatcag
      961 gcagagggcg tttctcatat ttccaacacg gaactgctga cttgtgattg tgatgtgctg
     1021 gtgccggcgg ctctggaaaa ccagatcaac gccgaagttg cagaacagct tcgctgccgg
     1081 tacatcgtag agggtgccaa cggccctacc actgtggaag cagatgcgat tctggaacag
     1141 cgtggcatta ctctgattcc ggatattttc gccaacagcg gcggcgtgat cgtgtcctat
     1201 ttcgaatggg tgcagaatat ccaggaactg acgtgggaac gctcacaggt caacgagatg
     1261 ctggaaaaac tgatgacggc ttctttcggc gaactgctgg aagaacgaaa aaagacggca
     1321 tgttcttatc gtatggcagc ttatatcgta gcactgcggc ggctgctgta cgccgaggac
     1381 atcaagggac tgttcccata atcgcaggaa gcgggagtgt agaagggaaa cactgcataa
     1441 agcccgtctg acggctttgc acgcaatctg cttgcagcgt ttccgtagaa aaagagaggt
     1501 gttgcatcat gacaggcaat gttgcattca tgttcgtatg taccattatg gtattcttta
     1561 tgacaccggg gctggcattc ttttacggcg gcatggtaag acggaaaaac gccctgaaca
     1621 ccatgatgtt ttgcatggca atgatgggaa ttggtatgct gctgtggttt ctctgcggct
     1681 attcgctgag cttcggcaag gatcatgccg gcattatcgg cgacctgaac aatgtgctga
     1741 tgcggaatgt ttcgtgggaa gtcaccggcg gagagggcga tgcaccggaa atggtgtctg
     1801 ctctgttcaa catgatgttc gcagtgatct cgccggcaat cattctggga gccgtggcag
     1861 aacgcatgaa attttccaaa atgatgttgt tcgtggtgat ctggtcgttc ctggtatatt
     1921 acccgctggc acatatggca tggggcggcg gactgctgga gatgatcggt tccattgact
     1981 tcgccggcgg caacgtcatt catatcagca ccggtgtttc cggactgacg gcatgcatca
     2041 ttctgggcag ccggaaaggc tacggagcca tgtcctacca cccgcacaac ataccgttgt
     2101 ttctgctggg agcctccatg ctgtggatgg gctggttctg cttcaactgc ggctgtgccg
     2161 gtggtgccaa cagtatcgcc gttctggcgt ttgccaatac agcactttcc tccgccgcaa
     2221 gcatggtggt atggatgctc atcgaagcat tggtgcagaa aaagtgcacc gtcatgggtg
     2281 ccgtgaccgg cggcatcgtg gggctggtcg gcatcacgcc gggagcaggc tatgtgccgc
     2341 tgtggtcgtc cctgatcatc ggtgcaacgg cagctccgat ctgctatctg gcaatttcca
     2401 aggtgaagaa aaagttcggc tatgacgacg cactggacgc attcggctgc cacggtgtcg
     2461 gcggtatctg gggcggtatc gtgactggta ttttcgccac caaacagatg aacgatgcgg
     2521 ttccgcggga aggcttgctg ttcggagatt accggctgtt tctctcccag ctgggggcaa
     2581 tcggcatttc cattgcagtg gcagtggtca tgacgactat tatcatgctg gtactgaaaa
     2641 agtgcggcgg tgtgcgggtg tctgatctgg aagaagcaca ggggctggat ctgatcgaac
     2701 acggcgaacg tgcatatccg gcgttcaacg gactggattg aggaggtgcg agatcatgaa
     2761 aaaaatagaa gtgattttcc gaccggaaaa gctggagccg ctgaaagaaa agctgctggc
     2821 agccggtgtg cagggtgtga ctatttatca ggtgcacggc tgcggaaacc agcacggctg
     2881 gatcgcctat caccgcggca atgaggtcat gatgaacacg ctcccgaagg tctatctgat
     2941 gacagtggtg ccggacgacc gcgtggagga cttcctgcaa ctgatcaagg aaaacgtcta
     3001 taccggaact gtgggcgacg gcaagatctt catttccact gtggacgagt gtattcgtat
     3061 ccggacaaat gaaaccggcg acgcggcact ttgacaggaa acaaaaggca ataccgtgca
     3121 aagtcgccag acgctctttg tgcggtattg ccaaaagcaa ctataaactg tacatattgg
     3181 aatatggaag aaagcacggt ctgcatctgg cagttcgtgc ttttttcggt ggtgggacaa
     3241 ctcgatactg tttgtagagg gcgatgccca catcgccccg cggtcgactg ctttatttcg
     3301 caaatcaact gtcatatttg caattttcac ttcaatatgg tatactataa aagtaacacg
     3361 aaaagcaaca cggcacaatg cccgaccgga ggtgaaccca tgcccatttc catatccacc
     3421 ggcagaaaaa tgcagatgtt tctgctggat ctgctctatc ccaatcgctg cccctgctgt
     3481 aactgttcta tttcttggcg ggaatatctc tgcgaagcct gccggcagga agtgactgcg
     3541 gaaccggcag acgcattttg tcccctttgc ggaaagctgc tgcgggactg cttctgcggc
     3601 acaggtctgt cctatgaccg tgccgcgatt ctgacagaat acaccggagc cgcccgtgcc
     3661 ggtgtgctgt ccatgaagtc cgcggcaagt ctgcacttcg gcagattctg cgggaacgtg
     3721 ctgggacagc ggatcgccga agatgcggtg ctgtccggct atgacttggt tattcctgta
     3781 cccatgcagc tgcggaaaca gcggaaacgc tgctgtaatc ctgccgcagt atttgccagg
     3841 gagatcgccg ctgtcacgcg gattccctac cggcaggaca tactgcacga caacggtacc
     3901 ggcaagacgc agcacaccct ttccgccgcc gaacgccata aaaatgtgga acagttttct
     3961 gccgctccgg tatcgctgga aggcatgcgg gttctgctgt gcgatgatgt actcaccacc
     4021 ggcagcacca tgaactgctg tgccgccttg ctgaaagcac agggtgcggc agaagtggct
     4081 gccatatcca tgaccacaac tgcaatgaaa tcaccctcaa aagaacccac acacggctga
     4141 tttccgatac ggacaatggc ccgccagaaa ggaaaccgac atgaattgga ttctactgtt
     4201 actcgctcta gtgatgatcg cctgtattct ctgcaacaag ctgaccagta agatcggcat
     4261 gcccgtgctg ctggcatttc ttgccgtggg tatgctctgc ggcgtggacg gtccgctgca
     4321 tatccgtttc gacaactacg ccctgacgga aaccctctgt accgtcgccc tgatctttat
     4381 catcttttac ggcggattcg gcaccaaatg gcgtgcggca aaaccagttg ccggaaaagc
     4441 ggtgctgctg tccactctgg gcgtgttcct cacagctgcg gcaatgggcg tgttctgccg
     4501 gtttgctctg cacaccggct ggctggaggg catgctcatg ggggcagtgc ttggctctac
     4561 agatgccgcc tccgtctttt ccatactccg ttccaaaaag ctggatctga aatacggaac
     4621 ggcttctctg ctggaagtgg aaagcggcag taacgaccct gtggcatacc tgatgactac
     4681 cattgtgctt gccatgatga ccacagaaat ttccgccggc agaatgctgt acatgatttt
     4741 cgctcagatc gtctacgggg tggggatcgg tgccgttctg gcagtctgca cggtcttttt
     4801 cctgcggcgg ttccggtttg aaacgtccgg cttcgatacc gtttttatgg ctgccattgc
     4861 gattttgtcc tatgtgctgc cggtacttat cggcggcaac ggctatctga gtgcctatat
     4921 cgccggcatc attctgggca accagaagat tcccaacaaa aagaatcagg tgcatttttt
     4981 tgacggcgtg acgggattta tgcagctgat ggtgtttttc ctgctggggc tgctctgcac
     5041 gccgtcgaag ctggggagcg tgatcctgcc ggcactggcg atcgcggtgg tgctgacatt
     5101 tgtggcacgt ccgctggtcg tgggcgtgct gctgacaccg tttcgggcaa aactgccgca
     5161 gcagcttctg gtttcatggg caggactgcg tggggcaacg tctgcgatct ttgccctgac
     5221 cgcggtggca agcggcgtac atttgcaata cgatctgttt catctggtat tctgtgtggt
     5281 gctgttttcc attgccctgc aagggacact gctgccgctg gtttcccgaa agctgcacat
     5341 gctggacgat tccacacacg ttctgaaaac ctttaccgac tataccgaag aaaaggaact
     5401 acagctgctg cggctgccgc tggaggcggg aaacagttgg gtaggggctg cggtcagtac
     5461 tctgacactg ccgccggaaa ccattctggc agcagtcctt cgcggcggcg actgtctggc
     5521 accgcgggga gataccgtgc tctgtgccgg tgatgtggca gtgctggctg ccggacactg
     5581 cggcgaaaag ctgcggcata tccagctgaa cgaactggaa attacatcgg aacacgactg
     5641 gaacggcaag acactggcag cactgaagct tccgaaagaa gagctggtga tccatatccg
     5701 ccgcggaaaa gaaacggtga tcccacaggg aagcacggaa attcttgccg gtgatgtgct
     5761 ggtcatgtac tgctccgaaa aaactgcttg aaagatgtga gagaatttga aaaagtggac
     5821 tccgaaacat gtaaactggc tcaaggcact gaaaattgct gccggagccg tggcggcgat
     5881 tctgctggca gaagcttttg gcttgcagaa tgccgcttct gccggtatca tcacgctgct
     5941 gactgtacag aacacccgac gggaaactgt catgagcagt gtgcggcggt tcagcggctt
     6001 tgcaatgatg acgctgctct gcctgccaat ctatcatttc tgcggcacag caccgtgggc
     6061 attcggcatc gtgttgctgt tgctgctgct gatttgctac agcttccgca tggacgatgc
     6121 cacgccgatc aacgccgtca tggcaactca ctacatgctt gccggcggtg tcactctgca
     6181 catggtgggg aatgaagtgc tgctgctggt catcggcagc ggcatcggcg tttgcctgaa
     6241 ctggttcatg ccgcggaatc tggggaaaat ccacatcatg cagcgggaac tggacgagga
     6301 gatccgcggg attctggaac gcatgtccat gcggctcctg gaggcagacc ataccggcta
     6361 cggggacagc tgctttgccg aaaccgaacg gctctccggc gaactgcggc aggagatcga
     6421 cgcattcctg caaaaccaga cgtggcagga cgacatttat tttatgcggt atgtggagat
     6481 gcggcgggaa caatgccgtg tgttgcaggt gatctatcgt cagctgctgc ggctgaatca
     6541 ggtgccggaa caggcgaaac cgctgtccgc atttctggca gatattgcga tgcatttcca
     6601 tgagggaaac gactgttctg ccctgttgga acagctggaa tccctgtacg cggcataccg
     6661 gcaggacgaa ctgccggaca cgcgggaaag ctttgagaac cgtgcaattc tttacagcat
     6721 tctgacagaa ttgcagtctt tcctacagat caagcggcag ttctatctga ccctgccgga
     6781 gcaggaacgc aagcagatgt tcgaacggct gatgcaggca tgattattgt gcggtgctga
     6841 tcggttcgat cgcggcaatg ttgccggaca attccggata gacggaaaca gttccggcgg
     6901 ttctggcggc atcagagagc gattcggttt ccacaatgaa ctccagcacg ttggcaccgt
     6961 ctgcacagct gcggtagcgg tgggtgctgt cggtgtgctg gtctgccttg atgtcgccca
     7021 cgcggacggt aaacacagag ccgctttcca acgtcacctg aaaccggtcg ccgcagccct
     7081 ctgtcagcca gcctgtcccc atggcaacca gatagtcatc acagcttcgc cgcagcccgc
     7141 ggctgtctgt ccacgcattc tgctgtatgg cgtactgttc ggaactggtg tccgtaatgg
     7201 tgcggtagtc catgtaggct ttgaatgcgg tatcgtattc ctgaaaaaat gaatctgttt
     7261 ccggcggtgt ctgcggtgct tctgtagatg ccaccgtggt ttctgtggtg ataactgtgg
     7321 tgcatgttga agtagttgtc gctgtggttt ccgttgtggt ttcgagagat gtgccgctgg
     7381 ttgcgagcgg cggtgctgtt gtaagcggtg ttgccgttgg cggcgggaca gcggcagcag
     7441 attctgtctt gcagaccgga acagcagcgg ctgtggaaac cggcggggca gacagcggca
     7501 ccgtatcacg aacggcagtg ctcacatcgg gggcaattgc ctgtgtacgg ggcagtgcgg
     7561 cttcactcca cgccggcacc tgctgaacag tcagcgtgca gaaacagccg gcacagaaca
     7621 cggaaaacaa actggaaaat cgaaacatac aagacctcct gacaaaatca gatccattgg
     7681 cggcgttgtc caagcatatg cgaatcagca gcatggatat gcagaaacgc tttcggattt
     7741 gtggattctg atttgaagta aactgttgaa actgtgcaga atgatgaaat tggcgaaaat
     7801 tttgcaaaac gccttgacaa atccggtgga ttcgcgtata ctgaaggcag ttaaattcca
     7861 tgagcaaaag taagtagccg gcaaaacggt agcacagaga gtcgggggtg gtggaatccc
     7921 gatgtatacg ctgtctggtg aatgggtttg tgagaagcga ggcgaacggg cagatgcccc
     7981 agtacctgat gccgcatctc ctgcgttatc gggagaaaga tatatccggt aaatacggcg
     8041 tatctgaaaa gagggaaacg aatgcttatc gtttctgaat ttgggtggca acacgtagac
     8101 aacaagtctg acgtcccata ggtgtgggac atcaggcttt ttttatttgc ataaaggagt
     8161 gaatgcgtca tgttaaaacc atcattggag gaagtcaagg cactggcaaa acagtataac
     8221 accattcccg tgttctatga gttttctgcc gacaatcaga ccccgctgaa catctacgcg
     8281 gctctttcga aagacagccg gaattcgttt attctggaaa gcgtcagcaa cggtgtgcag
     8341 tgggaccggt attcgtttgt tggcgtttcc ccgaaagagg agatctgcat taccggtaat
     8401 accgcagaga tccgcactgg tgacaagatc gaaacgaaag aagtggaaca gcctattgcc
     8461 tttctgaaag aacgcatggc agcatatcgc tctccggtgc tggagggtgc tcccaatttc
     8521 accggcggtc tgatcggcta tttcggatac gacaccgtcc gctacatgga aaagaaactg
     8581 actcatgtgc cggaagatga cattcacatg ccggactgcc atctgttcct gtatgaggaa
     8641 ctgctggcat acgaccacct caccagcaac ggcattgtca ttctaaacct gcacacggac
     8701 ggtgatctcg gagcacagta cgcggcggca cagtggagag ctgaagaact ggcagacatt
     8761 gtccgccact ataatccgca gccgcaggta cggcagcatc acggagaggc tgtggtacgc
     8821 tccaacctca ccaaggaaca gtacagccag atggtacggg acgcaaagga gtatatccgg
     8881 aacggcgata ttttccagat cgtgctttcc cagcggttcg agatcagcaa tccgccggac
     8941 agcttttccg tttaccggaa actccgtgcc acgaacccgt caccgtatct gtactatttc
     9001 cacacaccgg aatatgacgt ggcaggggct tcaccggaaa tgctggcaaa ggtgacaaac
     9061 ggtgtaattc acaaccgtcc aattgccggc accaagccca gagggcggac accggaagaa
     9121 gacatcgcca acgaaaaggc acttgccgca gacccgaagg aacgtgcaga gcacaccatg
     9181 ctggtggatc tgggacgaaa tgatgtggga aaggtttccg aattcggcag cgtaaaagtg
     9241 acaagatata tggtgacaga acgtgcatcg aaagtcatgc atctggtttc tgatgtggag
     9301 ggcaagcttc gcaaagacca gaccgcactg gacgcactca tggcgattct gccggcagga
     9361 acgctgtccg gtgctccgaa agtacgtgct atggaactga tcgaccagtt cgagaacaaa
     9421 aagcgtggac tttacggcgg caccgtaggg tatctgggat ttgacggaaa catcaatacc
     9481 tgtattgcaa ttcgcaccgt gctgtttgcc aacgaaaagg catatgtaca ggcgggtgcc
     9541 ggtattgtgg cagattctgt gccggaaaat gagtattacg aaaccgtcaa caaggcgttg
     9601 gcagtcatca atgccattaa ggaggcagaa caatgatctt actgattgat aactatgatt
     9661 cgttcaccta taatctgtat cagtatatcg gtgaactgta ccgtgacatt caggtagttc
     9721 gcaacgacga gatcaccatt gagcagatcg aagctctgca cccgcaggca ctgatcctct
     9781 ctccgggacc gggatacccg aaagatgccg gcatcaccat ggaggcgatc cgccacttca
     9841 acggcaagat tccgatgctg ggcgtgtgtc tgggacatca gtccatttgt gaagctttcg
     9901 gcggaacaat tgtccgtgct tcccacctga tgcatggcaa ggcaagccag atggtactgg
     9961 atatgacctg ccccattttt gcaggactgc ccaagacggt ttcctgcggc agataccatt
    10021 ctctgatcgc acaggaatcg gattttccgg catgcctgaa agtgactgca cgggacgaga
    10081 acggacagat catggcactg gaacatcggg aaacccagac ctatggcgta cagttccacc
    10141 ccgaatccct gctgaccggc gatggtaagc gtatgctggc aaacttcctg aatctgattc
    10201 cggacgtatc tgtgccgctg ccgcccattg tggaagaacg acctaagacg gaactgaaaa
    10261 agtatatcgc acaggtgacc gacggcaagg atctgacaga agcagaagca caggacgcaa
    10321 tggacatcat catgagcggc agtgcgacca atgcacagat cgccggtctg ctggtggctc
    10381 tccgcatgaa aggcgaaacc attgcagaga tcaccggttt tgccaagggc atgcggcaga
    10441 aggccaagca tgtcaccggc tgcgagaact ccattgagat cgtgggaacc ggcggcgatc
    10501 tggcgaactc gtttaacatc tccaccacat cttcctttgt tatctctgcg gcaggcatgc
    10561 cggtggcaaa gcacggcaac cggagtgttt ccagtaaaag cggtgcggca gatgtgctgg
    10621 aaagtctggg cgtgaagatt cagaccacac cggagcaggc aaaggcttcc attcaggaga
    10681 tcggtatctc gttcctgttt gcacagagtt atcacggtgc tatgaagtac gtggctccgg
    10741 cacgaaagga actggggatc cgtaccgtat tcaatgtgct gggacctctg accaatccgg
    10801 catcgaccga ttatatcctt ctgggcgtat acgaaaaggc actgctgccg gtaatggcac
    10861 gggtgcttat ggggctgggc atcaagaatg cgatgatcgt ctacggcaac gaccggctgg
    10921 atgaggtgtc catttgcgat aagacctccg tggcagaggt acgggacggc aaaatcacag
    10981 agtatgagat cgctccggag gacttcggct tcccgcgggc agacaagtcg gaaattgtcg
    11041 gcggcgtggc agatgaaaac gccaagatcg cacggggcat tctggacggc tccattcagg
    11101 gggcaaagcg gaatatcgtt ctcatgaacg ccggctgtgc cctgtatgtg tccggcaagg
    11161 cagccaccat tgcagacggc attcagctgg ctgcccggca gatcgacagc ggcagggcgt
    11221 tggcaaagtt acagcagctg gtggaattca ccaatcggaa tgtgtgaggt gcggaaatga
    11281 ttttagatga gatcatgaag tatcgtgccc ggcagctgga acgggaaaag gctgcctacg
    11341 gactggaaga aatgcagaag cgtgcggaaa aggcactggc ggagcgaaag ccggtttctc
    11401 tgaaagcggc actgcggcag gatacgctgt cctgcatttg tgaagtgaaa aaagcgtccc
    11461 cgtccaaggg actgattcgg gcagattttc agccggtgga aattgcaaag gcgtatgaag
    11521 ccgccggagc gagtgccatt tcctgcctga cggaggagca ttactttcaa ggctcgtccg
    11581 catatctgac ggcgatccgg caggtggttt ctctgccggt cctgcggaag gattttctct
    11641 ttgatgcata tcaggtttac gaggcggctg ccattggggc agatgcagtg cttttgatcg
    11701 ccgccgtgct gccgccggaa acgctggcgg aactgtaccg gctggcgtat tcgctggggc
    11761 tggaagttct cgtagaggtg cacaatcagg aggaactgga acagatcgca tttctggaac
    11821 cgcagattct gggtgtgaac aaccggaatc tgaaaacctt tgaagtcagt ctggacaccg
    11881 tggcaaagct gaaacccttt gcaccggagc aggcagtgtt tgtatcggaa agcggtatcc
    11941 ggaacaatgc cgatatgcaa atggtgcggc agctgggagc agatgcggtg ctgatcggag
    12001 aaacactgat gcgtagcgat gatattgccg gaacgctgca ccagctgcgg gagggcgtat
    12061 gaaaatcaag ttttgcggaa tccggcggct ggaagatgtg gcgttctgca acgaactcca
    12121 gccggaatat ctgggcatga tcttgtcatc aggatttcgc cggacgattt cgccggaaac
    12181 ggcagaacag ctggtaaaag ccaaaagccg cagcattgct gcagtcggcg tgttcgtcaa
    12241 cgaaacgccg gagcacattg cacaggtgct gaaacaggtg ccgctggacg tgatacagct
    12301 gcacggcagt gaaacggcag agatcgtttc cggtgtgcga tggcgtaccg gtttaccggt
    12361 atggaaagcg gtgcaggtgc aaactgccgc cgatatacag gcagctcggc agctgggagc
    12421 agaccagctg attctggagg gcaaagctgg cggcatgggc attaccgcag actgggaact
    12481 gatcgccgga gcaaaaccgg cacagccgtt tctgctggct ggcggactga caccggagaa
    12541 tgtggcagaa gccgcagcca gggtacagcc ggacggcgtg gacttttcca gcggcatcga
    12601 aaccggcagt gtaaaagact atgcaaaaat gaagcgaatt gtaacgataa tacgaggtgt
    12661 ataacatggg caaggtagga agatttggta ttttcggcgg gcagtatgtg ccggaaaccg
    12721 ttatgaatgc agtgcatgaa ctggaagaag cttatgcgaa atacggcaat gatccggaat
    12781 ttctggaaga ataccggaca ctgctgcggg actatgccgg cagaccgtca atgctgtact
    12841 atgcagaccg catgaccaga gatctgggcg gctgcaagat ctacctgaag cgggaagacc
    12901 tgaaccacac cggtgcacac aagatcaaca acgtgctggg acagatcttg ctggcaaaga
    12961 aaatgggcaa gaagcggatc attgccgaaa ccggtgccgg acagcacggc gtggcaactg
    13021 caactgcttg tgcaatgttt gatatggaat gcgagatcta tatgggcaaa gaggacgttc
    13081 gccgtcagcg gctgaacgta taccgtatgg aactgctggg tgcaaaggtc aacagcgtgg
    13141 acaacggcac tgcaactctg aaagatgcga tcaacgaggc gatgcgtgac tgggcaacca
    13201 atattgacac gactttctac ctgatcggct ctgtcatggg accgcacccg tatccgacca
    13261 tggtgcggga tttccagaag gtcattggcg aggaagccaa gaaacagctg atggaaaaag
    13321 agggaagact gccggattgt gtggttgcct gcgtcggcgg cggctccaat gcaatgggta
    13381 tgttctacga tttcattccg gacgagagcg ttcgtctggt gggtgcggaa gcagccggaa
    13441 agggtattga taccaagctg cacgctgcaa cagttgccaa gggcagcctc ggcattttcc
    13501 acggcatgaa gtcctacttc cttcagaatg aagaggggca gatcgcaccg gtttactcca
    13561 tttccgctgg tctggactat ccgggtatcg gaccggagca cgcaaacctc tataagaccg
    13621 gacgtgcgga atatgtgccg atcactgacg aagaagctgt acaggctctg gaatatctgt
    13681 cccgcacaga gggcatcatt ccggcaatcg aatctgccca tgctgtggct gcggcactga
    13741 agatcgcacc ggagatgaaa cccgatcaga tcatgatcat caatatttcc ggcagaggag
    13801 ataaggacat gcagcagatt gcagaataca gaggagtgaa gattgatgac taagaataag
    13861 attgatgcaa gactggaaca gtgcaaggca gagggcagaa aagccctgat cacatttatc
    13921 actgctggtg acggcggcta tgactgcacc gaaaaggcag tgctggaaat ggcgaaaaac
    13981 ggagcagacc tgatcgaact gggcgtaccg ttttccgatc cgatcgcaga gggaccggtg
    14041 atccagtgtg ccagcgaacg ctccctgcgg aacggtacca ctctggtggg tattttcgat
    14101 ctggtacgcc gtctgcggaa agaaaccgat gtgccgctgc tgttgatgat gtacctgaac
    14161 accattttct gtttcggaac cgaccgtttt ttcgcactct gtgaggaatg cggcattgac
    14221 ggtgtcattg taccggacat tccctttgag gaaaaagacg agattcaggg cagtgcagat
    14281 gcccacaatg tacacaacat cagtcttgta acaccggcaa gtgaagatcg tatccagatg
    14341 atcgcttcgg aagcaaccgg tttcctgtac tgtgtatcct ccaacggtgt aactggtgtt
    14401 cgcagccagt tccagacgga tttcgcacca ttctttgaga agatccacaa gtacgcaaag
    14461 gttccctgtg ctgtaggatt tggcatttcc ggtccggaag cagccaggga gatgagtcag
    14521 tactgcgacg gcgtgattgt cggcagtgcc gttgtgcggc tggtggaaca gtacggtagc
    14581 gatgcaccga aggagatcgg ggcatttacc aagagcttgc gtgacgcact ggacgcgtaa
    14641 gctttctggt gataattttc aaaaaaagat tgacaaccca ccggaaatct gctatactat
    14701 aactatcgat aatttttttc agcgatgaac ggagtgactg ctgcggaagc tgcacagaga
    14761 gggcgtgtct ggtgagaacg ttccggctgg ggcacaggtg ctgtccggga gctgtttcgg
    14821 gaaaccgaaa cgtatttcct gcgttaaagg agcacgaggg aagtgtctgt acatcagcac
    14881 ttctgaattt gggtggtaac acgaaggctt tcgtcccatt tggcggcgaa agcctttttt
    14941 gtatacagga gggaatgcgt atgctgccaa tggactgcca cacccacacc ggcatttctc
    15001 ccgacggttc gggcacggtt gccggacatg tggaacaggc gaaaaagctg gggctgccag
    15061 tgctggcctt cacggaacat gtggagatga accgctattt tccgcaggca cactacaata
    15121 ccctgccgcg gaacgagtgg gagatctttg accatgccgc cgtgctggaa cgatcgctgg
    15181 aagctgtcac tgccgcaaag gaacaggcag ctgcagccgg tctgacactg ctgtgcggca
    15241 tggaaatggg gcagcccaat gcagatttcg ggctgtcggc tgctgttgga gcagatgtac
    15301 ggctggactt tatcattgcc tcgctgcatg aactgctgga tcgaccggat ttcttctgcc
    15361 tggattattc acaggagaac attccggctc tcatggagca gtacttcgac caactctatg
    15421 acatctgccg ctggggaaag tttgacgtgc tgggacacct gacctacccg cttcgctata
    15481 tcgaagggga agcaggaatt cccgtgccgc tggaaccgta tctggaacag attcgctgct
    15541 gctttcgggc actggcggaa aacggcaaag gtattgaact gaacacttcc ggctatcggc
    15601 agaagtacgg caagccgttt cctacgttgg aatacctgaa actctatcgg gaacagggcg
    15661 gcgaggtgct gacctttggc tctgacgcac actgtgcaga agatctggca aaaggcattg
    15721 cagagggggt ggaactggca aaggctgccg gcttcacacg agcctgctat tttgtacaac
    15781 gaaaaccggt ctatatcaca ctttgactgg tttttcaaat tatttttcag gaaaggctgg
    15841 gtttctcatg aaagaacaat tactggaatt attgcagcag aacgcccgac tcaccaacgc
    15901 ggagctggcg gttatgctgg acacaacaga agcagaagtc gccgacgcga tccgagcgtt
    15961 ggaaaatgac ggcgtgatca agggctatag tgccattgta gatgacgaac tggcaaacaa
    16021 ggacatggta acggcaatca ttgaactgcg tgttaccccg cagcgggaca gcggctatga
    16081 ggagatcgcc cataccatta tgcagtatga cgaggtggag agcattgctc tgatggcagg
    16141 tgcctacgac ctttccgtga ctgtgcgggg aactaatgtg aaagacgttt ccatgtttgt
    16201 ttcgcagcgg ctggcaacgc tggcaggcgt attgtccact gcgacctatt ttgtactgaa
    16261 aaaatacaag gaaaaaggta tcattattga aggcgaagaa aaggacgaac ggggctacta
    16321 tttcccgtga tgtccgacag gaaaggggag aaaactcgtg ttgaattatg acacgcttct
    16381 ttcagatcag gtaaagaaaa tgaagccctc tggtatccgg aaatttttcg atctggctgc
    16441 catgatggag ggcgtgatca gtctgggtgt cggagagccg gactttcaga caccgtggac
    16501 agtacgaaaa gctgccgtgg acagtctgga acggaaacgc atggtgtaca ctgccaattc
    16561 cggtatggca gatctgcgaa aagagatctg tcattatctg aaccgcaagt accacctgac
    16621 ttatcatccg gaaaacgaaa cattggttac agtgggcggt tcggaggcga tcgatcttgc
    16681 gattcgtgca accgtcaatc cgggagatga agttctgatc gtagagccgt ctttcgtttg
    16741 ctataaaccg atcgtggaac tgatgcacgg tgtagcagtg ccgatctcca ccaagctgaa
    16801 aaacaagttc aagctgacac cggaggagct gcgttcccat atcacagaac gcacaaagat
    16861 gctgatcctg ccctatccga acaaccccac cggcggcatt atgaccagag aagatctgga
    16921 ggcgattgcg gaagttctgc gggatactaa tatcttggtg ctgtccgatg agatctactc
    16981 ggaactgact tatggcagaa agcatgtttc tattgccgaa atcgacggca tgtgggaacg
    17041 gacgatttat gtcagcggtt tctcgaaagc atttgccatg accggctggc ggctgggcta
    17101 tgtctgtgct ccggaaccga ttgcgaaaca gatgctgaaa attcaccagt atgccattat
    17161 gagtgctccc acattgagcc agtatgccgc tattgtggca ctgcgggaat gcgatcagga
    17221 agtgcagaaa atggtggacg aatacaaccg ccgccgccgc atgctggtag acggctttaa
    17281 ccgcattggt ctgacctgct ttaatccgga gggagcgttt tacatgttcc cctgtatccg
    17341 aagcaccggc atgaccagtg aggaattctg cgaaacgctg ttgcaggaag aaaaagttgc
    17401 cgtggtaccg ggcaatgcgt tcggagaaag cggtgagggt tttgtgcgtg tttcctatgc
    17461 gtactcactg aagcatttgc aggaagcact ggttcgcatt gaacgttttg tccagcggca
    17521 tcagggggaa aatcaatgcg agcagtaacc agaacggctc cggcaaaaat caatctgggg
    17581 ctggatatta caggcactcg tgcagacggg tatcatcttc tggaaaccgt gtttcaggca
    17641 gtttctattg cggacacggt gacagttgcc atgacggaat caccggaaat cacactgacc
    17701 tgtgatgtgc cggcagtgcc gtgcagcccg aaaaatattg cgtggaaagc ggcacagcgg
    17761 tatcgggagg cagccggctt gcagtccggt gtgcagatac agatcctcaa gcggattcct
    17821 atggaagccg gtatgggcgg cggcagtaca gatggtgctg cggtgctgct ggcattgcag
    17881 gaactgacag ggaatgctct gccgcaggaa aagctgctgg aaattgcagt ttcacttggg
    17941 gcagatgtgc cgtttttcct ttacggcggc actgcctatg gggctggcat cggagaaaag
    18001 ctggaaccgc tgccgctgtt ttccacagat tgtctggtaa tcgccaaggg taccgcaggc
    18061 gtatccacgg cacaggcata cggggcaatc gatgccctgc aaaatccgaa acacccacca
    18121 gtgcagcagc tgcgaaaggc gttggaagcc ggcggaactg ctgcggaaat tgctccgctt
    18181 tgcggtaatt tgtttgaact ggcggtggaa ctggaagaag tacagcagat tcgcagcacc
    18241 atgctggaac agggggcact ttgcagtgtc atgaccggca gcggtgcggc agtgttcggc
    18301 ttgttcccga atcgggaatc tgcggaatct gcctgtgcag ttctgtggaa acaggtaccg
    18361 tttgcggaag tttgtcacac attataaggg caatcccgca caaagattgt gcggtattgc
    18421 ctaagaaaat acaaaacgcc ctgtgtcaac gaaaatcagt tgacacaggg cgttttttgc
    18481 tattataaat tacaaattgc agaaattagt tcagaatcat tgcactgaat gcagtgtcca
    18541 gagccttttc cttgactgca ccagcttcgg tagtgtccgc tgtgacggtg tagtaagcct
    18601 tgatcttcgg ctctgtaccg gacggacgaa taacaacctt tgcaccttct tccagagaga
    18661 atgccagtac gttggacttc ggcagggtga tctctgtttt ttcaccggtg gtcaggttct
    18721 tggaaatgct ggtctggtag tcgtcgaact ggagcacctt cagaccgttg atctcagtcg
    18781 ggtgttcttc ccgcagctta gccatgatct tctgcatttc gatcatgccg ctttcgccct
    18841 caaaggtgaa gctgtgctgg ctgtgtacat atacgccgta cttctcgtac atacgcttgc
    18901 gggcttcgat catggaaatg cccttggtgc ggtagtaagc agccatttcg caaatcagca
    18961 tggaggcaac aactgcgtcc ttgtcgcgga cataggaacc agccagatag ccgtagcttt
    19021 cttcgaaacc gaaaatgtag cggttttctt cgcccttggc ttcgaggaag ccgatctgtt
    19081 cgccgatgaa cttgaagccg gtcagcacgt cgatgatctc cacgccgtat tccttggcaa
    19141 tggcattgac aatatcagtg gtaacaattg tcttgactgc gatcgggttc ttcggcatag
    19201 tgcccttttc ggttctctgc tggcagatgt attccagcag cattgcaccg acttcgttgc
    19261 cggagaacag tgcatagtca tcgccgtcag gaactgcaat gccaacacgg tcagcgtccg
    19321 gatcggttgc cagcagcaga tccggcttta cttcgcggca gagtttcaga ccgcattcca
    19381 gagcctcgcg gatttccgga ttcggatacg gacaggtggt gaagttgccg tccggatttt
    19441 cctgttccgg aacaacagtt acgtccttga tgccgattgc agacagaatg tggcgaaccg
    19501 gcttgtttcc ggtaccgttc agcggcgtgt aaacgatttt cagaccgctt tttgcacaca
    19561 gttccggatt caggctctgc ttcagaacgt cctgataata agcatcaatg acagactgcg
    19621 gcgtgtagga gatcatgccg gaagccattg cttcgtcgaa gttggtgtgc ttgacaccag
    19681 tgaacatatc cagagcgttg atctctgcca gaacggcgtt ggctgcatca atggtcatct
    19741 gacagccgtc gctgccgtat accttataac cattgtactt tgcagggttg tggctggcgg
    19801 taaccatgat accggcactg cacttctgtg cacgaactgc ataggacagc atcggggtcg
    19861 gcatcagttc tgtatagatg tgtacgcgaa tgccgtttgc agccagaact tctgcagcag
    19921 cttttgcgaa aacatcggat ttgatacggc tgtcatagga aatggcaacg ctgggtgctt
    19981 cgaaagcgga cttgacataa ttggcaagac cctgtgtcgc acggcgtact gtataaatgt
    20041 tcatacgata ggtgcctgca ccgataacgc cccgcagacc accggtaccg aattccagat
    20101 cccgataaaa acggtcactg atcgcagcgt cgtcaccagc gattcctttt aattccgttt
    20161 gcagatcggc atcttctgtt gcatgctcca gccactgctg atacaattct ttttccgtca
    20221 ttataggata cctccatgaa aacaatttac aataatcagg gaacaatacc gcgaaaatac
    20281 acccatagac gggcggctgc gataccggaa tgtgtagcct tataagaatc acacacccaa
    20341 actgtattta ttataccaca ttctctgtca agatgcaacg attttttttc gtgcaaacgt
    20401 gaattttttc agaaaacaaa aaaacagcaa agttacgcta tgattttgcg ggaaaatctg
    20461 atttgtgttt gggccatggg cgggctctta ctatatccat ataatagtgt tgtacattgt
    20521 cttatatgat ggcagcaccg ttgcttgtgc gatttgcata gttttttgca aaattctttt
    20581 acagatattt acttgacaaa tgcgaaaacg catggtataa taataaagc
//
LOCUS       DAWBGR010000019        20067 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_927_length_20067_cov_4.215821, whole genome shotgun sequence.
ACCESSION   DAWBGR010000019 DAWBGR010000000
VERSION     DAWBGR010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 20067)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20067)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20067
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_927_length_20067_cov_4.215821"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5214 a         4679 c         5501 g         4673 t
ORIGIN      
        1 gaaatgaaga aatgtcaaca actgtaaata ttgaaaaata atactccaag cacaaagcgg
       61 taaatcatat accctaacag catacagcac tctttcattt ttgaaggagt gctgtatcaa
      121 tttttagaca ggtatgaatg gtgcaagtca tgtattcatc acgctgaaaa atatttttgt
      181 acagtggaaa atgctgaaat atcgcgggaa agagtaatcc tgtggtgttg aaaatcagaa
      241 gaaaggcacc tcttagaata gaggtgcctt tttcagtttc caaggcttgc ggttagctac
      301 aactaatcaa aattagggcg attggtaaga aatttacaga aaaaagcttg attcttgtcg
      361 ggagatggtg tataatagtt cctgtatgcg aatcgtatac aatgccatat catgaaagga
      421 gtttgaccat gatttattca aacgaagtaa acgaaatgtg ctgcgtaaaa cagggcgtac
      481 agcacggtgc tgcaccgatt cctgaggaag caaagtgggt aaaggcaaag gaaatctctg
      541 acatttccgg tctgacacac ggtgtgggct ggtgtgcacc gcagcagggt gcatgtaagc
      601 tgactctgaa tgtaaaggaa ggcgtgatcc aggaggctct ggtggaaacc atcggatgtt
      661 ccggtatgac ccattccgct gctatggctg cagaagttct gccgggcaga accattctgg
      721 aggctctgaa cacagacctc gtttgtgatg caatcaacac tgctatgcgt gagctgttcc
      781 tgcagatcgt atacggccgt acacagagtg cgttctctga agaaggtctg ccgatcggtg
      841 ctggtctgga agatctgggt aagggtctgc gttctcaggt tggtaccatg tacagcacac
      901 tgaagaaggg tccgcgttat ctggaaatga ccgacggtta tgttaccaag atcgctctgg
      961 acgaagacga tgagatcatc ggctacaagt ttgtaaactt cggcaagatg atggacttca
     1021 tcaaggctgg cgacgatgca aacactgctt tcgaaaaggc acagggtcag tatggccgtg
     1081 ttgctgatgc agtgaagttt gttgacccga gaaaagaata aggaacagga ggaaacaacc
     1141 catggcatta tttgaatcat atgagagaag agaaaagcag gttctggctg ttctgaagga
     1201 atatggcatt gattccatcg aaggtgcagc tgaagttacc aaggctgctg gtctggacgt
     1261 atataagatg gtagaaggca ttcagccgat ctgcttcgaa aacgcaaagt gggcttatac
     1321 cgttggtgca gcaattgcga tcaagaaggg ctgccgcaga gctgctgacg ctgctgctgc
     1381 aatcggtgaa ggtctgcagg cattctgcat tccgggttct gttgctgacc agagaaaggt
     1441 tggtctgggc cacggtaatc tgggcaagat gctgctggaa gaagatacca agtgcttcgc
     1501 atttctggca ggtcacgaat ccttcgctgc cgcagaaggt gcgatcggta tcgctgaaaa
     1561 ggcaaacaag gttcgtaagg aaccgctgcg tgttatcctg aacggtctgg gcaaggacgc
     1621 tgcacagatc atcgcacgtg taaacggctt cacttatgta gaaaccgaaa tggactacta
     1681 caccggcgaa gtcaaggaag tattccgcaa gtgctactcc aaggacgcaa acagcccccg
     1741 tgcaaaggta aactgctacg gtgcaaatga cgttaccgaa ggcgttgcaa tcatgtggaa
     1801 ggaaggcgta gacgtttcca tcactggtaa ctccaccaac ccgacacgtt tccagcatcc
     1861 ggtagctggt acttacaaga aggaatgcat cgaaaagggc aagaagtact tctctgttgc
     1921 ttccggcggc ggtacaggcc gtaccctgca tccggacaac atggcagcag gtccggcttc
     1981 ctatggtatg accgatacca tgggacgcat gcactctgat gcacagttcg caggttcttc
     2041 ctccgttccg gctcatgtag aaatgatggg tctgatcggc atgggcaaca acccgatggt
     2101 tggtgcgact gttgctgttg cagtatctgt tgaggaagct gctaacgctg gcaagttcta
     2161 agaaattgcg tggtttttcc atgtaaatag aaaaagaggg cacctcggct ggttttggct
     2221 ggggtgtcct tttgcgtttt tacagctgtt tattgaccac attttgacca catacacgct
     2281 ccaaagcaag ccctgcaaaa aatacaaaac gcccccgaac aaaaaagaat cccaagcgaa
     2341 accggcaaat aaactggaat ccgtcggaaa agccggcagt ccattgacgg cagagctgtt
     2401 tttgtggtat actgttgcag gaggtgtgtg gctgatgcat atatttgacg tactggggcc
     2461 ggtgatgatc ggaccgtcca gttctcatac tgccggagcc gtgaaaatcg gctatgtcac
     2521 gcgaaagctg ctgggagaag caccggtgca ggcgaacatt ctcctgcatg gttcgtttgc
     2581 ggataccggc tcgggacatg gtaccgaccg tgctgtgatt gccggactgc tgggctgtaa
     2641 gcccgatgac ccgcagattc cgtccagctt tgaagcggca aaaaaagccg gcttttcctt
     2701 tcagatcacc aggggtacac tgcggggagc tcaccccaac acagtgaaga tcgaagttgt
     2761 cggagaacac ggcagccagc tggaagtgat cggttcgtcc gtaggcggcg gccgcattcg
     2821 catttgcgag atcgacggga tccggacaga cttttccggc gaatacaaca ccattatcgt
     2881 acacaacacc gatacccccg gacacgtggc acaggtgacc acagttctgg cacagaaaaa
     2941 cgtgaatatc gccgccatgc agctgtaccg caataccaag ggcggctatg cggtaatgat
     3001 tctggagtgt gacgaccgga taccgccgga catggttcgc tggctcagcg gactggacgg
     3061 cattctgaaa atcaccagct ataatgcaga ggagaacgtg tgatatggaa ctgcggtctg
     3121 ttgcggcttt gatagaggca agccggaaaa tgcccatctg ggaagcgatc ctttgcagcg
     3181 atctggaaga agatacgccg gaacagcggg cggcttcgct ggagaaaatg caaatgctct
     3241 ggcaggtgat gcaggactcc gccgccggct atgacggttc tcagcgttcc ctcagcgggc
     3301 tttccggcgg ggacggggag aagtatactc agatgctgtc ctctggcaag atgatgtgcg
     3361 ggacatacct caacgatgcc attgccaccg ccctgaaagt cagcgagtgt aatgcctgta
     3421 tgaaacgcat tgtggcagca cctactgccg gctcctgcgg ggtgattccg gcggtgctga
     3481 ttccctaccg gaaagaatac accgatacac cggagaagga gatgctccac gccctctacg
     3541 tgaccggcgg ctttggaaat atcattgctg agcgggcatc ggtttccggt gcggaagccg
     3601 gctgtcaggc agagatcgga gccgcttcgg ctatggcagc agcggcactg gtctacctgc
     3661 ggggcggctc accggaagcc tgcggcaatg cctgtgccat ggcactgaag aatctgctgg
     3721 ggctggtgtg tgatccggtt gccggactgg tggaagtgcc ctgcgtcaag cggaatgtgg
     3781 tgggcgtggt gaatgccatt tcctgtgcca atatggcgtt ggcaggtatt gtcagccgga
     3841 ttccgccgga cgaagtcatt gatgcaatgg cagaggtggg cagcagcatg tctgccgacc
     3901 tgcgggaaac ggcaagaggc ggtctggcgg caacgcctac cggaaagcgt atcgctgcgg
     3961 agcgagcaga gtaaaggcaa cactgtgggt gctgccgaaa gagaaagagg aaacatttat
     4021 gaaacttgtg acttatttac agaacggaat agaacagatc ggcgtgctga caccggcaga
     4081 agatgcggtt gtgccggtag gcatgctggg gctggatttt gcagatatgg cagaactggc
     4141 agtgcagatg accgatgccc agcgggacac ggcggcggct ctgctgccgg aaaaagccgg
     4201 agagggaatc cctctggaac aggtgaagct gctggcaccg attccccgac cggcacggga
     4261 catcatctgt ctgggcgtga acttttccga acacgccaag gaaagcgtag gcgttatggg
     4321 gcaggaatac ggcagcgaac ggaagttccc gatctatttc gccaagcatg tcaacgaggc
     4381 agtcgctccg ggggacagca tcaacggaca ctttgacatt gtcaccgatc tggactacga
     4441 agtggaaatg gcattcgtcg tgcggaaaga cattcagggt gccagggcgg aggacgcggc
     4501 ttcctatgtg ttcggctaca ccattatgaa cgatgtcagt gcacggtgca ttcagatgcg
     4561 gcataagcag tggttccgcg gcaaaagtct ggacggcttt gcaccgatgg gcccttgcct
     4621 tgtgacggca gacagctttg actttccgcc gaagctgaat ctcacctgta aagtcaacgg
     4681 ggaactgcgg cagaagtgca acacggaact gtatatccac aacattccct atgtcattga
     4741 ggaactgtcc cacgccatga aaattcccgc aggcacgatc atttccatgg gaacgccggc
     4801 aggtgtcggt atgggcttcg tgccgccgaa gtatctgaag cacggcgatg tggtagaatg
     4861 tgaagtagag ggcatcggaa aaatcatcaa tccggtagaa taataaaata gaataataaa
     4921 aaccaaaacg ccccgcatgc gaagggatga aaccgcatgc ggggcgttgt ttccggaacg
     4981 tatcccgtgt gtggagaaag ttcacacaga cacattcctt aaaagagaaa atggaaagtt
     5041 gtcaatggac aagtaggtta tgaaaacaca ctctggaact gcttactgta aatcgcccaa
     5101 ggaaactaca gttttctgac cgtagcccag gatgtcagca tacaggtctt gcagttcgtc
     5161 ctcatccatg aggaatgcca cagttgcctc gacactttcg cccggagcaa cattggtgat
     5221 gttgttcttg ctgtggtctc tgtcagtggt aaacaggaag aactcaccat acttcaagtc
     5281 gtccaatgtg ccattgcagt acatggtttc atccgtcggc tcgcaggcaa ccgcgccgta
     5341 atccagtctg tcaccgttct tctggagcag actcgggcag acacagatct cctgtgtgat
     5401 gctgcactca ttggtgtaag tgagatgcat cacaaggaca cgctggggaa cggtttcttc
     5461 cttgattttg gtatccagtg tgttcacgcc gtcgccgcgg gagtaccatg tgcgtacatc
     5521 gtccttgaca gtgccgtccg cattgagata ctggctgtag tctgtcggat tgccgatttc
     5581 atccactgcc ggaagtccgt caaagttatc ctggacggaa acgctgtcca gcttgacagt
     5641 gacgcgggaa tatctgtcat catcatcata gaaatccgaa cgaatggtgt caccgatctg
     5701 ctgaatggta tagtcatcga ccttgtaaac ctcatcggtt ccgccagtct cttcctgcgg
     5761 ctcaccgctc cacagctcag ctgtctcctt gtcagaaggt gtcagactga gattttctgc
     5821 gatcttttcg atttcttcct ttgtcacgcc ggttgttgca tagagctgta cggcatagtt
     5881 ggaatccggg aatgcgaccc agacatcctt gtcaaaaaca tccttgccgg acagtcccgt
     5941 ggtgtcagtc actgcataga gaacagtatt gctgccgcag gtgagtgtct cctgagaaac
     6001 gctgtagttg acggtgtgcg tcagagcagc ttcctgactg tcaatcttat agaagaacat
     6061 acttatggcg cgtccgctgg ggtctctgta ctttccgctg ctgggatcgg ttgccatgtc
     6121 ttccggcatc cagcccacct gcaacgccat gatctggtca gaaacgctgt cgttcttcgg
     6181 aatcaccatt tcctgctgat actcaccgcc gccgttctgg aaatagccgc ggatctgagt
     6241 ggatgcatat acagcggtag ggatgactac gatggctgcg gcaactgcta cggctgcggt
     6301 cagggacttt ttcatgtgga atttatgcga tctcatagga ctttcctcca tctggtgaag
     6361 catctgctgt ttccggcggg catagcgttc cgaaaaaacg tggggctgtg cgtctgatgt
     6421 aaatgcgtcg ttgacgatgt tgttccattc ggtgtcgtta tacggaaaag agttgtgttc
     6481 ggatttcatc ataaagttcc cccttttttt gttggattgc ctttctggca cgttcaaagc
     6541 gttttcttgc agcggcttct ttcatagaga gccgctcggc aatttcgcga aaggataact
     6601 cctcctcgca ccgcatcagg acgatctgcc ggtccaggtc gtttagcggc tctaacattt
     6661 cggcaatgga ttcttcctgt gcaatgttgg cgagaagctg ttccatgtca tctttctggt
     6721 tggggacctg caaaatgcgg tcgtcccaga cggtatagcg ttcggaatcc cgttggttct
     6781 gccggtattg gttgatcgct gtgctgcgaa tgatcgaaat gatgtaatgc ttggtttttt
     6841 cgctgcgaac gtcgccgatc tttttcagat tctttaggat tcggaggaac gcatcggata
     6901 cagcatcttc cgcctgttct gtatgatgca ggattgcgta agcgatgcga tacatgggct
     6961 gttcgtagat gtcatagagc agcttgagtt tttcctgttc tttttctttg tgcgtagcca
     7021 tttgctcacc tctttctgaa acgggctgtc attcctgtgc atcggtcttt cgcccgtctg
     7081 catatcataa ccgtctggcg gtatcgattt gtgacagggt tccaaaaatt ttaaggcaat
     7141 accgcacaaa gcccgcctga cggctttgtg cggtgttgcc ttaagagaac ttcaaatcac
     7201 agctgcactg gtctttcgcc cgtctgcatt gcatattata accgtctggc ggtatcagtt
     7261 tgtgacataa ggagaatctt ttagccagtg caatgcatta ccagttcatt ttgaaaattg
     7321 atttgtgtga acacacagca agtccccaca tgcagattac cttttgcata tggggacttg
     7381 tatcatacaa aaaatcctgt aacgagagct gctgccgagg ctgccagagc caccacgatc
     7441 aggggcaggt tccagtacgc cagtaaaact gcgacgactg ttcccacggc tgctgtgatc
     7501 atgttgcctg tggagtagaa aatggcagga atggtcatgg cactgagcac tgcgtaggga
     7561 atgtaataga ggaagctttg cagaaaatgg ttctggatct ttttccggaa aaccgtaaac
     7621 ggcacaacac ggatcagata ggtcacgcct gccataatgg caatataaag aatcaggctc
     7681 atgtgcgttc ctcctccgga atgggaaaga cgatcgcccc gaatatcgat gccagaactg
     7741 cacaaagaat aatggcgaag ccgctggaaa ccgccgtgaa cacgtaccgg aacaatatgc
     7801 tgcacgccgc cgccagagcg accactgcca gcacgctttt tttcttgcgg gaaaccggaa
     7861 cgatgattgc caggaacatg ccgtacagca cgatacccat ggcatcaatg ataaacttgg
     7921 gcagaatttg tcccgctgct gcccccagca gtgttcccag tgtccagccc agaaatgcga
     7981 tcaggatcag cccgtacatg taggcggggc aaatggtgcc ggactgtgcg gaggctacgg
     8041 cgaaaatttc gtcggtgatg ccgaatgccg ccgccatgcg ttgggggagc cggaacgttt
     8101 tgtccagctt ctgggacagg gaaaagccca tgagggcata ccggaagtta atggtcagct
     8161 gagtcagagc catttccaga taggaaccgc cggcagcgat cacgcctacg ccggcagcct
     8221 gtccggcaga ggtgacattg gtaatggaga ttgccacggc ggcagaaacc gacagccctg
     8281 cacgcactgc catgatgccg aatccaaagg aaacggagag gtatcccaat gcaatgggga
     8341 ttccctgtaa aatgcctttt cgaaatgctt tgtccaatgt gatgatgtcc tttccggagc
     8401 ctgcggacag gctcatgtaa aatgtcacag ccaaaacggc acagtaattt ttattatacc
     8461 acaatccgac gggaaatgca aattttttca gcatttgatt ttctaaggaa aaatgtaaaa
     8521 ttttcttgtg tttttgtgtg tggtatggta taataagaaa gacgggaacg gggagttttt
     8581 gggaaaatcg ggatttatag gagataagtt atgagcaaaa taacagcttt actgcaaaag
     8641 atgaaatctc tgtgtaacag taataatggt aaggacacat ttaattctcc ggtgacagcg
     8701 gaagaaatat ctgattggga gattgcacac aacgtaaagc tttgtgatga actgaaagaa
     8761 ttttatcttt tcagtaatgg tatgaattta cgtatctatt tctcaacatt caatatctgt
     8821 ccgtttgaaa aaattacctt tagagaaggc ggcttattgg gatatggaga atttgaaggc
     8881 tatatgaaaa taggtgattt tgtgggagat ggttcaataa tatgtataga cagaaattat
     8941 catgtttgct gtatatttga aggtgatgct gaaataacca aatgctcact tacagaacta
     9001 attgaaagag aacttgaaca tcttgaagaa aaaatcgagt acagaaaatg aaaagagaaa
     9061 aataacgaag tccttatacg taaattccag tttgcaaaat aaatcaacag agaaatcatg
     9121 gaggcaggca tgaaaagcaa aatcatatat tgcatcaatt ttatttggac aagctgcgtt
     9181 gcgttttcgt ttccgatatg ctttggtttg atttatctca acataactgg tcatgcaaaa
     9241 ggctatagct atgatttagg atcagagaaa gatgtttcta tcatgttggg ctgtgtggaa
     9301 cttctcattt ggcttgccct tgcactgccc tcaaatatct atatctttcg gaaaacactg
     9361 cgtaaaggca aagcatattt gctgataccg atcgcatggt acatagtcct tgctgtaatt
     9421 tgcctgatga tcacattcgg tgggtggtca gaatacgcaa aagaagtatt tcatgtcaga
     9481 aaaattgaat taaatggaaa cgaggacact gatattgtct tttacaatgg aactgaatat
     9541 ctttccggac ttttttattg tgcagatgat gacaggagga tcattggacg tatagatcat
     9601 ggtgctagag tctatacggt tggttcagat acatcgccgc agtatcttct aattgtaggc
     9661 agagataata gcggcacctt tattgcagaa ggtgcttctg ttccaacaag tggaaaaatc
     9721 accaaaatac tgatcgatcc cggaattcgc agcgataatt cacagtatct ttcatctgct
     9781 gatgagatcg ctgtgattga tgaaataaca aatctatcag gcgaattcca gacatttcgt
     9841 gtggataatt attacacaaa tggcaatgcc ttttactatg tatacaataa cagtaatgtg
     9901 tcatgcaatg aaaattatgg tggctatatt gcttttaccg aggggaaatg gatatatgct
     9961 ccacctgaaa acagacctgt atggacaggc gaatgcaatg gtgtaacgat agaagccctt
    10021 gttattgatg atgaggagat cattgaaaaa atgtgcagga ctgacatggt gaaatatata
    10081 gattatcaaa agtgattata gggtatccat ttgcggcatc tgtgcaaaac gcaacaaaac
    10141 aacacatatt ctatatgcaa cctgacgaaa gcaaaaagaa gatttctaaa tcacttgact
    10201 tttgcataga aatgtggtat aattcatatc aaacagatag gtaatgtagc agttaccgga
    10261 aagggagtca tatcatgaaa gtttatcaga aaatcacgga tttgatcggc ggaacaccgc
    10321 tgctggaact gacgaatctg gaggcagagg acggactgcc ggcaaagatc tacggcaagc
    10381 tggaatattt caatccggct ggcagcgtca aggacagaat cgccaagggc atgatcgatg
    10441 atgcagaggc aaagggtctg ctgaagccgg gcagtgtcat cattgaaccc accagcggca
    10501 ataccggaat tggtctggct tccattgcgg cagccagagg ctatcgtctg attattgcaa
    10561 tgccggaaac catgagcgtt gaacgccgca acctgatgaa agcatacggt gcagaactgg
    10621 tgctgacaga gggtgccaag ggcatgagcg gtgcgatcgc caaggcaaag gaactggcag
    10681 cggagatccc caacagcttc attccgtcgc agttcaccaa tccggcaaat ccggacgtgc
    10741 atgaaaagac caccggcgtg gagatctggg acgacacaga cggtacagta gacattttcg
    10801 ttgccggcgt aggcactggc ggtacggtta ccggcgtggg cagatacctg aagtccaaga
    10861 atcccaacgt caaggtcgtt gcagtagaac cgaagggttc tccggtgctg tccgagggca
    10921 cagctggtcc gcataagatt cagggtatcg gtgccggatt tgtgccggat acactggata
    10981 ccagcgtgta tgatgaagtc attgccgtag aaaacgaaga tgcgttcgaa atcggtagag
    11041 ccattgccag aagcgagggc gtgctggtag gcatttcctc cggtgcagct gtctgggcag
    11101 caattcagct ggcaaagcgt ccggaaaaca agggcaagac cattgtggca ctgctgccgg
    11161 ataccggtga aagatacctg tccacaccga tgttttctta agaagttcct atgagcgaaa
    11221 caaaaccaaa cacaccagca gagatcctgt atcattttat agagggacag ctggaactgg
    11281 aaccggagca ctatccgtac accgtcgcac agataacggc tctggaaaca aaggtgaaag
    11341 ccaacacgcc gctgaccgat gcagaatcgg aaacactgcg gaaagtgctt cagacctaca
    11401 tggaactgta cgagtacagc gaagccgagc aggaaaaagt gctctctctg ctttcacgct
    11461 aacagaatac aaataataca aataacagcc gcagggaaac cggatttccg ttccctgcgg
    11521 ctgttgtttt atttatgttg aaatgccggc agaaactgtt ggaaaaacgc ctccggtgtc
    11581 atcagcacac tggaagtgtc cgaacagggc gaaaagcaca gcagcttcga ttgccgcaat
    11641 gtgctgtctg cctgcatccg gcagaggtgc tcttggtcga acagcagtcc cataggggaa
    11701 agtgtgtggg gctggcagtg caaaatttca cagatctgct gtaattctgc cggctctgcg
    11761 ggagcatagg attccggcgg ggaaactgtt tccgcgtctg tacccagcat cagcaggaag
    11821 ctgcctgtgg tgctgacgaa aactctgcac accggaaaaa ccggctccag cttagatttt
    11881 ttcagactct gtatccactg tgtccggtca cgataggcgg ggtgacagaa gcgggtaaag
    11941 ggaatgtcct gccgcatcag ataggagtag agcaacagtg catctgcatt catggcaaat
    12001 ccgcctcctt ttccgcggga cagacgacgt tccgataata acgggtgaag tgcttcggcg
    12061 taatgcccgt gaattccttg aattcccgtt gaaaatgagc ctgatcggca taattcagca
    12121 ggtcaataaa actggaaacc gaatcggcag aatgctccat catgcggctg agagcatatt
    12181 gaaaccgtgc aatgcgggcg tacatcttcg gggaatagcc catggcatcg gaaaacatgc
    12241 gggtgaggtg gcgttcggaa taacagattt ctttggcaat gtccccgact tccacatcgc
    12301 cgttggcgtg actggtaagg tataagactt gctgcactgt ttcctcaact atatattgtt
    12361 ccggcgagaa tcggagcaga tattcctgga acagccgtgc ccgttcccag agggtatccg
    12421 tttcaaaaat ctgttccagc agcagtgcgg tatctgccgg aatctgcgga acaaaggttt
    12481 ccgtgttggt gatctctttc agacagacct cgtgattggg aaaaaacatg cccggtttca
    12541 ggcgtactcc gaaatagtct gcatgcggat acacgataag ggattttctg caaaatggag
    12601 aaccgatcag ttccattttg ttctgctggt tgtgtcggat aaagacgatg tccacacagc
    12661 cgtccggaat gcagcacatg aacaggttct cgttggtgcg gtcgatgatc ttgtgggtgg
    12721 agaattgata atatcgtgca gcaaaaggcg ttttttgaac tgcctcctga tagtgcttga
    12781 catcaaatga gaaaaaaggc tgccggatca taaagtcaga atagctttga tacagatttt
    12841 tcataggaat tctcctctcg gtgtaccagc gattgtaaca attaaaacta gggcaatacc
    12901 gcacaaagcc cgcctgacgg ctttgcacgc aatctgcgtg cagattttcc gccatattgc
    12961 ctagaaatcc cttgccatac ggcagtatgc ctgcggaatt tctaggcaat ctgccgaaaa
    13021 atctgacaac gcaccttacg cacaagcttt gtgcggtgtt gccctaggga taactgtcga
    13081 agtcagctaa gctacaatgt actggggcaa taccgcctgt cagctttgca cataatcttc
    13141 cggtgcatct tacgcacaaa ctttgtgcgg tgttgcctta agatattctt attataaaaa
    13201 ccgtggcgtt atttgtcaat cccgcacggc ggatttctgc tttttgtttt tgaatttgca
    13261 gtttcgcttt attatattag gcgttgtgcg ttgaatttcg gttagtttgt gataactaaa
    13321 attgttattt taagttttag cgtgtccgtt tttttcaata aaaccagccg gaatgggtgt
    13381 atactaagta cagaaacaag ggaaacgcaa agcgaaagcc acagcggcag aaacgcattt
    13441 gcggttccgg aggaacagaa aacgaagctg caaaggtgca tctgattcgg aagatgtgcc
    13501 tttttctcgt ttctgcccag tgcttctata gaaagacaat tgaggaggtc ttcgttatgc
    13561 gtattagaga gcatccgatt atcgaaagct atcacaaagg gaagcagatc acgtttactt
    13621 tcgacggaaa ggaactgact ggttatgagg gcgattccat tgctgcggcg ttaaaggcag
    13681 ccggcgtcat ggtacaccgc tacacagcga agcagcacaa gcccagaggc attttttgtg
    13741 ccattggcag atgtaccgac tgtgtcatga tcgtcaacgg cgttcccaat gtgcggactt
    13801 gtgtcactcc tctggaggcg ggcatgcaga tccagacgca atacggcgta gagccaaaga
    13861 aggaggactg acaatatgaa acggcatgaa ttgatcgtca tcggtgcagg acctgcgggt
    13921 ctgagtgcag cgatcgaagc aaagaaaaac ggcatggatg ttatcgtgtt cgatgaaaac
    13981 caacgccccg gcggacagct gttcaaacag atccacaagg agcatcatgc taagacccgt
    14041 ggatttatca ttggtcagca gcttctgaaa gaggcagagg atctgggcgt tcgggtagaa
    14101 ctggactcca ccgtaatggg tatctacgaa aataaggaag tcaccgtaaa gcagaacggt
    14161 gaagtccatc attataaagg cgatgcgatt atcatcgcca ccggtgctgc cgaaaacatg
    14221 atcccgttca agggctggaa cattccgggt gttatcggtg ccggtgcggc acagaccatg
    14281 atgaacattc agggtgtgca gccgggcagc aagattctca tggtcggctc cggtaacgtg
    14341 ggactggtcg tcagcttcca gctgctgcaa gccggctgtg aagtggcagc cattatcgat
    14401 gcggctccca gagtcggcgg ctatggtgtc catgcggcaa agctggcaag aaccggcgta
    14461 ccgttttatc tctcccacac cgttgtggag gcaaagggca cggatcgtgt taccggtgcg
    14521 gtgatcgcac aggtggacga gcacttccag cccattccgg gaacggaaaa ggaactggac
    14581 gtggatacca tttgtattgc cgttggtctg acaccaatgt ctcagctggc aagcaatgcc
    14641 acctgtgata tggaactgat tccccagaag ggcggtcatg tggcactgct gagcgaatac
    14701 ggcgaaactt ccgtagaagg catctattgt gccggtgacg ttgccggtat cgaggaagca
    14761 agttccgcaa tgattcaggg ccgaagcgtg gcttctcacg tcagcatgaa agccggctac
    14821 cttactgaag acgagtttga gagcaagtat cagggctatc aggaggctct gggacagctg
    14881 cggcagggca tgtttgcccc gaagaataag ggcagaaacg acttcacgga aactgatgag
    14941 ggctatccga tctccaagac gctgctggca cacggttata tgactgagga ggaactttcc
    15001 aagtttccgg cggcttctta ccagaaagcc ggtatccacc ctgtgatcga gtgtacccag
    15061 aatattccgt gtaacccgtg ccaggacgca tgtcatttcg gctgcatcaa ggtgggcgag
    15121 aatatcacac ggctgccggc aatcgatgag gaaaagaagt gcaccggctg tggcttgtgt
    15181 gtggcttcct gctccggtca ggcaatcttc ttggtggacg aaacctatga agaaggcttt
    15241 gcttccgtga cattccccta tgagttcctg cctatgccga agatcggcga taagggcaag
    15301 gctctggatc gtcagggcaa gccggtctgc gatgcagaga tcgttggcat caaaaagaca
    15361 ccgatcatgg acaagaccgc agtggtcacg atgaaggtgc cgctggaata tgtgcacgct
    15421 gcaagattct atcgggcaga ggtttgacga aaggaaggtg caggataatg cgtaaaatga
    15481 ttcgggttgc caatccggaa ccctttgtag aacgggaaga cgatgacatg atcatctgcc
    15541 ggtgtgagga gatcaccaag ggcgagatcc gtcgggcagt gtatgacggt atgcgtacca
    15601 ccaatgaagt gaagcggtat ctgcgttccg gcatggggct gtgtcagggg cagacctgcc
    15661 agcggctggt gcagggcatt atcgcttcgg aactgaaagt gcggccgtcc gagctgggca
    15721 tgattcaggg cagagcaccg gttcgtcccg tagagattcg gacgttcagc aaaaacgtga
    15781 cagatgtaaa ggaggactaa gccatgctgg aaaaacgaga agcagatgtg atcatcattg
    15841 gcggcggcat catgggctgt gcaacagcct accggctggc aaagcggggt gtcgatgttc
    15901 tggtactgga aaagaaagag atcggcaacg gcggttcctg ccgaaacgcc ggcggtgtcc
    15961 gccagtctgc acgtgatccg aaagaactgc cgctggcaat gtacgcggta cagaacatct
    16021 ggccgcagct gagcgaggaa ctgggcgttg atgtagaata tcatcagggc ggcaacctgc
    16081 gtctgggtct gacggacaag gacatcaagg ttctggaaaa ccacaccgcc aagagcacca
    16141 agctggggct ggaagtggaa atgatcagcg gcgacgaagc acgaaagctt tgtccgtaca
    16201 tgtccgaaga agtggttgcc gcaagctggt gtccctctga cggacacgcc aacccgctga
    16261 aagccacact gggcttttac cgcaaggcat tgcagctggg cgtgacattc gtcaccggtg
    16321 tcaaggtcat ggctctggaa aaggtcaaag gtcggatccg caaggtcatc acagacgatg
    16381 gtgtctatga agctgaccag atcctgctct gtgccggcta tgaatcccgc cgtatcgcca
    16441 ataccgtagg cattgatgct ccggtagaac gacagttcaa tgaagtactg gttaccgagg
    16501 cacagccgaa gatgttcgat attatgctgg gcgttgccgg cggcgagttc tacgggcacc
    16561 agtcggaaca cggttccttt gtgttgggcg gcaacagtgg cttgcaggcg tttacttcca
    16621 acaatgacaa tttcgtcacc aatagcctga ccgctccctg tatctcccgc ggtatcatca
    16681 agtatttccc gatcctggaa aatgtcaagg tggttcgtac atggtccggc tggtatgacc
    16741 agtgcattga tgtgctgccg gtgatcgaca acatcaagga agtgccgggt ctgactgtgg
    16801 cgttcggttt cagcgggcat ggctttggca tcagtccggc ggtttccatt gccgtcagtg
    16861 aactgatgct ggacggcaag tccaccacca ttgacatcag ccagctgacc tatgaccgtt
    16921 tccacgcaaa gggttaaact acatattgta agttgaaaaa atgaatcatt acattgcaag
    16981 gacaaaggcg tctatactac agacactttg tcctttttca attggagagg aggagaatcc
    17041 catgatactg cataatgcca ccgtagatac cattctggaa cggcgaagcg ttcgcagcta
    17101 tgcaccgcag cagatcacac cggacgaact ggaaacgatt ctggaagccg gacgatcagc
    17161 cccaagcggc atgaatcagc agtcggcaat tgccgtggca gtgcagagca aggcggaact
    17221 ggcacagctg gtggagctgg caaaggcggc agcccatcaa gaccacaacc cgttttacga
    17281 tgcacctact gtgattctgg tatttgccag gatgaccaat ctggcaccca tggaggacgg
    17341 ctgtacggcg atcgagaaca tgcttcttgc cgcgaaatca ctgggcatcg gtacctgctg
    17401 gatctatgcc gtcaatgctc tgtttgcttc tgatgccggt atggcgtggc aggatgcaca
    17461 tggtatcgga aaagagtacg ccattgtcgg ttcgctggcg gcagggtatc ccgccggagc
    17521 agaacctcag ccaaaggaac accatgcatt ttataaatgc atttaatgaa aatggaggaa
    17581 cagaccaatg agcagattta cattgccgag agatttgtac cacggaaagg gaacactgga
    17641 aaccctgaaa acactggagg gcaaaaaagc ttttcttgtt gtgggcggcg gttccatgaa
    17701 gcggtttgga tttctggaca aggcagtcgg ctatctgaaa gatgccggca tggaagtagc
    17761 actgtttgaa ggcgtagagt ctgatccgtc tgtagatact gtgctgcgtg gagcaaaagc
    17821 catgcaggag tttgctccgg actggatcgt ggcaatgggc ggcggttcgc ctatcgatgc
    17881 cgcaaaagca atgtgggtat tctatgaata tccggatacc aaattcgaag atctgtgcac
    17941 cccgttcagc ttcccgaagc tgcggacaaa ggcaaagttt tgtgcagtgc cctctacctc
    18001 cggcactgcc acagaagtga ccgccttttc cattatcaca gataacgaaa agggtatcaa
    18061 gtatccgctg gcggacttca acattacgcc ggacatcgca attgtggaca gtgcactggc
    18121 ggaaaccatg ccgccgaaac tgatcgcaca caccggtatg gacgcaatga ctcatgccat
    18181 tgaagcttac gtttccacgc tgcactgcaa ttataccgat gcagtggcac ttcacgccat
    18241 taagatgatt caccagtacc tgaaaaagtc ttatgacggc tgcatggaag cccgtgcggc
    18301 aatgcacgat gcacaatgcc ttgccggtat ggcattctcc aacgctctgc tgggaatcgt
    18361 ccactccatg gcacacaaga ccggtgccgc atacagcacc agccatatca ttcacggctg
    18421 tgccaatgcc atgtatctgc cgaaggtcat taagttcaat gccaaagacg aagccgcagc
    18481 caagcgttac gcggaaatcg cggagcacat tggtctggca ggcaattcca tagaagaact
    18541 ggtggattcc ctgatcgcag aattgcgttc catgaacgac aagctgaaca ttccccagtc
    18601 cattcagaac tacggagcag gcggtgtcaa ggcagaaacc tcgatcatcg acgaaaccga
    18661 gtttctcgac aaactgcctg aagtggcagc caatgccatt gccgatgcct gcaccggttc
    18721 taatccgcgt atcccctcgc aggaagaaat ggaacagctg ctgaaggctt gctattacga
    18781 tctggcagtg gatttttaag gaaacactgc aagcagacgg acaggcgata tatattggaa
    18841 caccttgttc ccaacccaat ttaaaggagt gtatcattat gagttatccg aaagttgaca
    18901 ttctgtatct gagtgaaaag gacatgctgg acgctggcgt aaatgatgtt gtgaagtgca
    18961 cagacaccat ggaagaagtg gtgaagcttc tggacgacgg agattaccgc atgggcggtg
    19021 aaaacggcaa ctctcacggc tgtatgatca gttttccgga taatcccgca ttcccgaaca
    19081 tgccgaagaa cggtccggac cgccgcttta tggcaatgcc ggcatatctg ggcggcaagt
    19141 tcgatatggc aggtgtgaag tggtacggtt ccaacgtgga gaaccgtgaa aagggactgc
    19201 cccgttccat tctgatggtc attctgaacg acaaggacac cggtgctccc aaggcattca
    19261 tgtccgcaaa cctaatcagt gcataccgta ccgcagccat tccgggcgtt ggcgtaaagt
    19321 tgctgtccaa gccggattcc aaggtgctgg gtgttgtagg accgggcttc atcaacaaca
    19381 tcaccattga aacctttgca gcacttcgtc cctcgctgga caccctgaag atcaagggac
    19441 gcggcagagc ctccattgac cgctgcattg cctatgtcaa ggaacactgc ccgcagttca
    19501 agaacatcat cgtctgtgat accatggagg aatgctgcaa ggacgcagac attatgacca
    19561 ttgccgtttc cacacctacc ggcggacaca ccaactatcc gtttatccag cgggaatggc
    19621 tgaagccggg ctgtctggtg tgtgcaccgg gtgccgtaga ttttccggac gaactggtct
    19681 gcgatccgaa tgtaaagctg gttgtagaca acaagaagct gtatgaggca tgggcagagg
    19741 aatatccgta tcccactttc gataagatgt tcctgctggg cgacaagttc accgacctcg
    19801 accatgacgg aaagctggac tggggcgtgg tacagaacat cggtgccatt gccaacggca
    19861 aggtgccggg cagagagtcc gacgatcaga tcattctgta ctccatcggc ggcatgccgg
    19921 tagaagatgt ggcatggggc aagaccgtgt atgaaacggc tctggaaaag ggcatcggca
    19981 cgaagctgaa cctgtgggac gcaccgtatt tgtattaagg caataccgca caaagagcgt
    20041 ctggcgactt tgcacgccat ctgcttg
//
LOCUS       DAWBGR010000020        19940 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_938_length_19940_cov_4.526025, whole genome shotgun sequence.
ACCESSION   DAWBGR010000020 DAWBGR010000000
VERSION     DAWBGR010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19940)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19940)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19940
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_938_length_19940_cov_4.526025"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4321 a         5766 c         4897 g         4956 t
ORIGIN      
        1 tccgcttttt gtttcatctg acgaaaaatt tgacgacgca tctgtcgcac aatccttgtg
       61 cggtattgcc cttacaatca acgccccgaa aggtcactgc ttttcggggc gtttttgaca
      121 aagccaaaaa atccccgctg cttttccaca gcggggattt tgtaaatcgt atccagttgt
      181 gcttagcggc gatccagaat gttgaagatt gccatcaggt cgtcatctac cggcttggac
      241 tgctgctgcg gtgcttcctg cttcggagca gctgccggct gtgccggtgc cggattcggt
      301 gtgctgccgc tgactttaac gtcatctacc agcgggttat ctaccggaat cggttcttct
      361 gccggagcag cttcttcctc cggtgcttct tcctcttccg gagcgtccgg atctaccata
      421 tcgaaaccag ccgcaattac agtaatcacc atttcgtcct gcatgtcttc cttgaatgcc
      481 gtaccgaaaa tgaattccac atcttctgct gccttgtcgg taatcatctt ggtggcaaca
      541 tctacgtcgg tagacataat gtcctcgctc attgcgatgt tgatcaacag acgctttgca
      601 ccggcaatcg aagtttccag cagcggactt gcgataacag cgttggcagc ttcctttgcc
      661 ttgtccttgc cggagccgtg accgattgcc atgtgggcat agccggcacc cttcataatg
      721 gtggaaacgt ctgcgaagtc cagattgatg tagccttctt ctacgatcag atcggaaatg
      781 ctcttgacac cagtcttcag cacgtcatct gccagtgcga aggattcccg catggtcagg
      841 ggcttgtcca aaccttccag cagcttttcg ttcgggatca cgatcaggga atccacatac
      901 ttccgcagtt ctgcgatacc tctttctgcc tgtaacatct tctgttcccg ttcaaacgca
      961 aacggctttg tgacaactgc gacagtcaga atgccggctt cctgtgccag acgtgcaacg
     1021 accggagccg caccggtacc ggtaccgccg cccatgccgg cagtgatgaa taccatgtca
     1081 gcacctttca gagctgcttc gatctcgtct ttgttttcct ctgcactttt ctgtccgact
     1141 tccggcttgt tgccggcacc gcggcctctg gtgagtttcg aaccgatctg gatcttggtg
     1201 gttgccttgg aattattcaa tgcttttgcg tcagtattga ctgcaatata ctcgatgtca
     1261 tgcacatcag aagatgccat gcagttcagg gcgttgccgc cgccgccgcc gacaccgatc
     1321 accttgatat ttacatcggg atcaaacgca tcttcataga taaaatcagt catctcaaca
     1381 tttcctccta caagaacttc tgtggtacgc tgtatatttt ccgcatcgtc cgtcttccgc
     1441 acatacggat accttcttta gaaatacact tattatttta acacatttcc gaaagcattg
     1501 caagcttttt tttgaaataa atcgggtttt tccctttccc acaagatttt aagggaaaat
     1561 ccgcctgaat ttcacaattt catacagatt ctgcaagaac attttcgtta ctgccagcct
     1621 gccgctgttg ttgtggtttc tgtctgggtt tctgtggtaa cgagattgcc gtattcgtcg
     1681 gtttccgtca caggatttcc gtattcgtcc gttgcagtgg tttgcagcgg tgccgtggtc
     1741 tgctgttcca ccgcctcctt gtcccgataa gaacactggt ggtcgcccac catagagaga
     1801 tagcccacct tgtcggggga cagctgcccc agcacagttt ccgccagcgt gatcttatag
     1861 tccatatcgc tccagtttcc caactgaaag gacactctgc cgtcaaagat caggttgatg
     1921 ttgtatttat ctgcgaggtc gatggaagtc accggacaat ccagcccctt ggcgatccgt
     1981 tcggacaggg actgaaagat cttgtccttc tggctgtccc gcgatgtcag cgttttcccc
     2041 ggctcccgca cggaagcttc gaagccggtg atcgtcggca gggacggatc actgtccgga
     2101 ccgctgacca gaatttttcc ggcggcactg acttgcagcg taccgctgga atccgtcaca
     2161 ttaaacgccg ccttgctggg ttcaatggtg atgaccagcc ggtcggggaa gtccttctgg
     2221 atcgaaacgc tgtccacaaa gatcagccgg ctcagaatgt tttccttgac cgtttcgcgg
     2281 ttcagccgca tcatattgtc accggtatgt acaccggaag cctttaccac gtcgtttttt
     2341 tgatacggct ggtcggcatc tccggcaacg acgatctgct caatattaaa gaacactgtc
     2401 atcgacagcg acacacccac accaatcacc agaattgcca ccagcagccc gtacagcggt
     2461 cggaaccgat tccggcggcg gcggcgtttc gaactggtct ggctctggat ctctgttttt
     2521 tccacgtcct gcatgacttc tgctcctcac ttcttttcta tggaaacact gcataaagcc
     2581 cgcctgacgg ctttgcacgc aatctgcaag cagattgcgt gcaaagcttt attcagtgtt
     2641 tccctatccg acacgttttg gtgtctgttc tgccggcgga ttccgataca ggggcagttt
     2701 ccacgcggga aatttttgcc ccaacgccct gcaatgcctg ctcgattgcc tcatatccgc
     2761 ggtcaatgtg tgcgatctgc cgcacctctg ttttcccctg tgctcccagc cccgccagca
     2821 ccatggcggc tccgccccgc agatcggtgg cggcaacgga agcaccgcta agagaacgca
     2881 caccggtgac tacggcaatt ctgccggaca cgcgaatttt cgcccccatt ttcacgagag
     2941 cgtccacatg acgatagcgg ttctcgaaga tgttttcttc cagcacgctg acaccgtccg
     3001 ccagactcag cattgccatg aagatcgcct gtgcatcggt gggaaaaccg gggtgaggca
     3061 tggtacggag aaacggcacc gcccgcagcc gccgcggtgc cagcaggtgc agccgatccg
     3121 gctccggaaa tacccgacag ccggtctgtt ccagcacagg gagcatattc tccatgggct
     3181 gggtgctgac accacgcagg cacagcgtcc cgccggtaat tgccgccgcc cccagatagg
     3241 tcacgccggc aatgcggtcg ggcatgatcg tgtgacggct gccccgcagc ttccggacac
     3301 cggagatcac aatggtgctt tcgccgtcgc cggagatgtc tgccccgcag ccccgcagaa
     3361 atgccgccag atcgcagatc tccggttctc ttgccgcatt gtgcagcacc gttctgccct
     3421 ctgccagcac cgccgccagc atgacatttt ccgtagctcc caccgagggg aacggcaggt
     3481 gtatgcctgc tccgtgcagt ccctccggtg cggtgcagtc cagctgtccc ccatgtacag
     3541 aaaaatgtgc acccatttgc cgcagagcat ctaagtgcat atcaatggga cggggaccca
     3601 actcacagcc gccgggatag gaaagatgac agctgccgga gcgtcccagc acagccccca
     3661 gaaaaatgat cgaagaccgc atctcctgca tcagcacttc ggggatcgta tccgcacaca
     3721 agtcccgcac ctgtactgtc acggtatgtt cctgcacggt acaccggcag cccaggtgtg
     3781 tcaggatccg gcatgccgca aacacatctg tcaggcgggg acaattttcc agaaccgttt
     3841 cgccgtcaca gagcagcgtt cccgccagaa tgggcagggc actgtttttt gccccatgga
     3901 gaaaaatttc tccctccagc ggtttgccgc cctcaatcac cagtttttgc aaactgcatc
     3961 acctcttttt attttcagtg tatgcagttt cgacagaatt ggtgaaatgt gcaggcaggt
     4021 gggctttcta cagtatttcc cgcggtctta tttggatttt gctttctgat acagctgggc
     4081 gaccacgttc cagatacggt tgtccgtgtc tgcaatttcc agcgacttcg ccttgtgccc
     4141 catttccgtc aggcgttccc gatcggcata gagctgttcc acttctgcaa tgatgcgttc
     4201 cggcgaagcg tctttctgtt caatgaccac agccgcaccg gcatcgccca gcacgttggc
     4261 gttgtgcagc tggtgattgc cggtgacgat gggggacggg atcagaatgg aaccccgtcc
     4321 ggcagcctcc agttccgcca gcgtggacgc accggaacgg cagatgacca gatctgccgc
     4381 cgccagacaa acgtccatgt cattgatgta ttccgtgatt cgcagacggt cgcctttcag
     4441 cggaatgcct gccgcagcca ttgccttggg gaaggtttcc tttcccatgc cgccgtagcc
     4501 gtgaatgtgg ttgatcgaca gccccttctg cacgtgccac ggaatcacct ttgccatcgc
     4561 ttcgttgatg cagcctgctc ccagactgcc gccgaacgag agaatgcaca tgccgtcatc
     4621 aaagcccagc tgcttccgtg ctgcggctct gtccatgccg gaaatgccgc cacgcaccgg
     4681 cagaccggtg acggtatacg gacagttcag gtggaagaat tccaacgcct tttccacagt
     4741 cagcatcacg tggtctactt cctttgccag catcttgttg gtgacaccgg ggaatgcgtt
     4801 ctgctcgtga atggcacagg gaatccccat gcgggctgcg gcgtggagga tcggccctgc
     4861 cacatagccg ccggtgccaa tggcaatgtc cggctggaac tgcttcagga tctgcttggc
     4921 acggtgctgc gaagttgcca gatagtacac cgccttacag ttccggcgaa tatttttcag
     4981 cgtcagcttc cgctgaaagc ctgccacatg catggtttcc atcggatacc ccgccgccgg
     5041 caccagacgt gcctccatgc cgttgggcgt tcccacaaac aggaactctg cggacgggtc
     5101 atgttttttg atgatcgagg caatggcaag tgctggattg atatgaccgc cagtgccgcc
     5161 tcctgctaat aatactttca cacgttacct ccctgcagtt gtacttctgc ctgtggtgct
     5221 ctctccgccg ccggtgcccg ctggggcgac tgataccgta cacgggagat gttcaggaca
     5281 atgcccattt ccgccagctg tagaatcaac gccgtaccgc cgtagctgaa aaagggcaga
     5341 ctgatgcccg tattgggaat caggttgctg acaaccgcaa tgttgaaaaa tgcctgtagt
     5401 ccgatatgag atgtcagacc aattgccatt aacatgccga acttatcctt ggcacgcata
     5461 gcaatataga atccgcgaat gaccagcagg gtaaacagca gcaccactgt gagtgctccc
     5521 accagcccca gttcctcgca gacgatagag aatacaaagt cgtttttggt ttccggcagg
     5581 tacagatact tctggcggga ttcccccagt cccagaccga acagtccgcc ggagccgatg
     5641 gcgatcaggg actgacaggt ctgccatgta ttgccctgac tgtctgcaaa cggatctacc
     5701 catgactgga tacgcttctg aatgtagtcg tagttgcggg acatgatcga aaccaccagc
     5761 agggcacccg ccgcaccgat aatgccgatc agcagaaact gcttgagatt gacaccgcct
     5821 acaaaaatca gaatgataca agtcatgacg atcagagccg ttgcagagat gtgcggttcc
     5881 ttgatgagca gtgccgcagg taccagcagt gccagcagca tcggcagcat gccccgcagg
     5941 aaataccgca tattgtcata gttcttggtg atgacatagg agtagaagat caccatgccg
     6001 atcttcatgg tttccgaagg ctgaaagttg aaactgccaa tggcgatcca gcgggtcgca
     6061 ccgccgtcag taacgccgat tcccggaata aacaccatga ccagcaggat cagcggcagc
     6121 acataagcac cgatcgcgat cagttttttc tgaaagaaat gatagtccac catgcacaaa
     6181 aacagcatca gcaccagacc cagtccggca tttttcatct ggctgacggc atagtaagaa
     6241 ccctcttttc cctcggcaat tgcccacgca tagctggcgg agaacatcat gacaatgccc
     6301 atgaccagca gtgcaatgac aatgaggaaa aacggcagat cgacttcccc gtactgtacc
     6361 ccacgcctcc agaaaagctg catatccccc agtctgcgtt tttttccggc agtttctttt
     6421 tccgccatat agaacctcct tctgccttac agcagtacca gcagcagtcc gcacagtccg
     6481 gtaactgcac cgaacagcga aaatgtgatc acaattttat actcactgaa tccgctcatc
     6541 tcaaagtggt ggtggatcgg tgtcatcttg aagatgcggc ggccttcgcc gtacttcttc
     6601 ttggtatact tgaaataagt cacctggatc ataacggaca gtgcttccag aatgtacacc
     6661 agagccacca gaatcatgct caggtgctgg tggcttgcca gtccgatagc tgccacagag
     6721 gcacccagat acatcgagcc ggtgtccccc atgaagcact tcgccgggtg caggttccac
     6781 accagaaagc ccagacagcc gcctgccagt gccatggtaa acagggtgta gcagcccatt
     6841 tgcagcttgc tgcaaatcag ggtaaagatc agcattgcca ccagtgtcac cgtgccgcac
     6901 agaccgtcca cgccgtcagt caggttcacc gcattggtca ggtacagaat taccagcacc
     6961 atcagcggat agtagaacca cgagatgttg aaccggacaa agcccagatc aatgtgggtg
     7021 tcccgatcgc ccagccagta cattgccgcc agccacagca ctgctgccac tacttgcagt
     7081 acgatcttct gcataggctt cagcccctcg ttctgcttct tggcaacctt tgcgaagtcg
     7141 tcgataaaac cgatcacgcc gaacagcaga cagaagatca tgcagctcaa cagccgcagt
     7201 gcctcccgat tactgaatgt ggaaacgaaa tcggtggtgc cgaaaaaacg gtacagggca
     7261 tagcccaccg cggtggcaat gatgctgccg aaaatgaaca gcagtccgcc catcgtaggc
     7321 gtaccctgct tgttggcgtg ccagctggga ccgaagtcca ccttaatggg ctgcccgaat
     7381 ttcagcttgt gcagccacgg caatagtgct tttcctccga gggctgccac gacaaacgcc
     7441 agcagtccca ccaatatgtt aatccagaac ggcatgatct gcgtcctcct cctgctcaaa
     7501 aatcacatcg atacaatcct ctaggtgcat gccgcggctt gccttgaaca agatcacatc
     7561 attgggacgg agatactgcc ggatcacgcg gcagagttcc cgtttgtcct cggtgtgata
     7621 cacggcaact tcctgcattg cggcacgctg tgccagataa aaagattctt tcccgtagca
     7681 gaacagagcg tccacttcac tttctgctgc catatcgcct accagtgtgt gcagctggcg
     7741 ggacatttcg cccagttcca gcatatcccc cagcacggcg attcgtctgc ccttgcacgg
     7801 cagatcccgc agcacagtca gagccgcttt catggagtca gggctggcat tataacagtc
     7861 tgcaataatg gtatatgcac cccgccggta aatattctgc cgcagtccta cggtgtgata
     7921 ctgctccaga gccttgcaga tctgttccgg tgcaatgcct gccagccgtc ccacgcagaa
     7981 cgccgcaagg gcattcatta cgttgtggtc accggcacag ggcagttcca ccggataggt
     8041 gctgccgtcc tgtgccagaa tggaaaagct ggtggactgt gcctttcgct ccacgtctgc
     8101 cgcacggaca tcagcctttt cgctgtgcat gccgtaggtg atgaccggac gggagagttc
     8161 ccgcttcagc ggtgccagca gcggatcatc gccgttgacc accagcggag catctgccgc
     8221 catgccgtcc tggatctcca gctttgcctg ccggatcccc tcctgtgtct tgaggttttc
     8281 gatgtgggaa tagccaatgt tggtaatgac accaatgctg ggttcggctg tccgtgccat
     8341 gcggctgatc tcgccgaagt gggacatgcc catttcaatg actgccgcct gatgacagtc
     8401 ctccagcccc agcagtgttt tcggctgtcc gatctcgttg ttcagattgc cctgtgtttt
     8461 cagggtgcag aactgggatt ccagtacgca ggcaatcatt tccttggtgg tcgtctttcc
     8521 cacgctgccg gtgatgccca ccagaatcgg gtgaaatttc tcccgataga accgtgcgat
     8581 ctgctgcaat gcccgaccgg tgtccttcac caccagacag gggcagtccc ccacctgccg
     8641 gtctgtgacg gctgccacgg ctcccgccgc gatcgcctgt tccacaaaag cgtggccgtc
     8701 aaatttttcc ccacgcagtg ccacaaacag acagcctgcc ggcaggtcac gggtgtctgt
     8761 gctgacacag gtgatctctg cttctaccgg cacagcacag tgcagcaccg cttccaactc
     8821 tgacaatttc caaggtttca tgtacaatat atcccccagt cctatttatc gcttcggtac
     8881 cagcggcagg cattcccgca cgatttcccg ttcgtccata tgaatatgta cgccgccttt
     8941 cagtacctga taatcctcat ggccttttcc ggcaagcaca atgacatctt ccggctgtgc
     9001 cagctgcatg ctccgcagaa tggcttcctt tcggtctgtt acgacctcat agggcacatc
     9061 gctgtcagtg agtccggcga gaatgtcggc aatgatcgcc tccggctcct catctctggg
     9121 gttgtcggaa gtgaccacca gaaaatcggc atacttcgcc gcagcctttg ccattttcgg
     9181 acgctttgtg gcatcgcggt tgccgccgca gccgaacaga cagatcagtc tgcctttggt
     9241 gtattccttg acactgcgga gaatattttc cacagcgtcc ggcgtgtggg catagtcgca
     9301 aatcacggtg aagtcccgtc cggtggggat cacttcgcag cggccccgca caccggtgca
     9361 ggtttccaga gccttgcgga catcgtccac cgacataccc acggcacagc atgcagccac
     9421 cgctgccagt gcattggaca cgttgaacat gccggtcagg tgcaggtcta ccggatagga
     9481 ctttccgccg cagcacagcc agaagctggt gccgtctgcc cgcagcttaa tggcatcggc
     9541 gtagaaatct gcctgattgc tgctgccgta ggcgattttc ggacaggtca cttcgctgta
     9601 cagccgcttg ccgtagtcat cgtctgtatc gatcactgcc gtatcgcatc ggctgaacag
     9661 cagtttcttt gcctgataat aattttccat ggtgccgtga tagtccaggt gatcctgcgt
     9721 caggttggta aacaccgccg ccgcgaaatg ggtttcgccg atccgctgct gcaccagccc
     9781 gaaagaagaa acctccatga ccacatcatc acagccagcc tctgccattt tcgcatacag
     9841 agccatcagg tcgtagacaa acggcgtggt gttgtcggtg tggacgatct cgtccccgat
     9901 ctcgttctga atggtaccga tcagccccac ttttctgccg ttttccgtga gaatatgctt
     9961 gatgagattg gtgatcgtgg tcttgccgtt ggtaccggtg acaccaatga aacgcatttt
    10021 ccgttccgga tgcccgaacc atgcggcaca gagttttccg tacagggcac gggtgtcctc
    10081 agtgaggatc tgccggttgc ccagtcccag atcatgatct accagaaccg ccgcggctcc
    10141 cttttccaga gcctccgctg cggcagtgtg tccgtcaaag cgttcacccc gaatgcaggc
    10201 gaacaccatg ccggactgca cctttctgga atcgtctgtc agtccggtga tctccagatt
    10261 ctccagactg gtttttgcat tgtcttccaa aagcgtatac aattgcattt ctctcatcct
    10321 ttctgtgcag tattgcctca aattacctct ataggatcag tccgttgcct caccggcgga
    10381 cagtgtcaaa gtcactgacg aaccacgctg gatctgggtg ccttccggta cagactggct
    10441 cttgaccagt gcaccctcgc tgctggcaga agctcccacc agagcgtagt tcaagccata
    10501 gtatgccatg gtttcattcg cctgtgaagc cgtcatgccc accagattcg gtacggaaac
    10561 cagatccggc gttgcattct gctcggtata cagcagcacc ttaccgccct tgactatgct
    10621 ggtgccggta gtcggggact gggaaatcac agaagaaccg ctgccgacaa cttcatatgt
    10681 cagccccagc tgttccagtg tggatttcgc cgtatccagc gttgcatctt gcagcagcgg
    10741 aacactgacg ttcatttcct tggcttcctc gtcagtatac tccggataga agcccatgta
    10801 cggaagaatt tcttccatga tctcctgtgc atagggcgtt acgaccttgg aaccgtagta
    10861 gccgatttcc ggattcggat agtccgcctg aatcagcata atgagttccg gatcttccgc
    10921 cggtgcaaag cagcagtacg atgccacata gtcatcttcg atgtctctgg tggcacgaat
    10981 ttccgctgta ccggacttac cgccgatgcg atagccctga atataggcat tgtgagaggg
    11041 gttggaggaa acgactgcct ccagctgctc ccgcatggtc tgagaggttt cttccgaaac
    11101 gatctgccgc ttcaccgttc tggtattttg cagcacggta ttgccgctgc tgtctattac
    11161 atggtctacc acatacggtt caaccacata gccgccgttg atcgccgcag agtacgcggt
    11221 aatcagttcc aacgccgtgg tttcgttggt ctgtccgtat gcaccggaag ccagatcgac
    11281 ctgtgtcatt ctatcctcag gcacgaccac gctggaaacc tcgcccggca gatcgatgcc
    11341 ggtcttttcc gtcagaccga agccctgcaa ataatagctg aacttctgca cgcccagccg
    11401 cagaccaatt tccataaagg cagggttgca ggagttggtc agtgcctgtg tgaacgtctg
    11461 cgagccgtga ccggaacgca gcgagcagcc gattttcgcg tcctgtacct gcaaatagcc
    11521 ggagcagaag aacgtgtcgc tgttcagatc gatcaggttt tcctcaaacg ccgatgccga
    11581 agtcacaatt ttaatggtag aacccggcgg gttgatctca ctgatcgact tgttccgcca
    11641 ctgtttttct ctggcttcca gatatgcgtc ctgatactcc gattccggca gttctttcag
    11701 ctgtgctgcc acaacgggat cgtagatctc cgagggattg ttcaggtcga aggacgggca
    11761 ggtcgccatg gcgtagatcg caccggtttt cgggttcatg atgatgccgg tggcacggtc
    11821 gttgacctcg aacttctcca ccatttccga catgctcttt tccagatagt actgcaaggt
    11881 ggagtccagt gtcagataca gggacgcacc gtcctccgca tcataggtgg tggaataccg
    11941 gtagggcatt tcctcaccgt tggcactctg tgccgaaatg attctgccgt ccacgccgga
    12001 gaggtagtcg ttgtactgat attccagacc atactgtccg tcgccgtcgc cgttggtgaa
    12061 gccgatgact gcagctgcca gttcattctg gggatagtac cgccgtgtgg tcgcctcctc
    12121 gccgataaag gacaacttca gtttatcgaa atatgtggtg atctcgtccg ccacatcttt
    12181 ttccacttgg gttttcagca cgtagtagcg accgcttttg gtataggatt cctccagctc
    12241 agcccgatcg atgcccagcg tttccaccag aaatgtggtg atggactgct tcagttgatc
    12301 cacgtccacc agatttgccg cttcttcgtt attcttcgcc gcagtttctt cctttttggc
    12361 gtttgccttt tcgattgcgt ccatttcgtc ctgatattgt ttggggtcga tgtacacgtt
    12421 gtacaccgtt gcactccacg ccagtgccgt gccgttggca tcataaatgg caccgcggtt
    12481 cgcccgcagg gtcatggtac cgaaatggta gttgttcgcc agcgtttcgt acttctggtt
    12541 atccaccacc gaaactttca gcagctttcc ggcaactgcc gcaccgcaga ccaccagaat
    12601 ggtcatgcag accgccgacc ggcgacgcat caccgcagat gcagaacgag gctttgcttc
    12661 tgttttcttt tccttttttt ctttccgctt tttatggatc atctccacgt gcagcacacc
    12721 tccgttttct gataccccga tgaacttctg ccggcaggca ttcgccgccg atgacatacc
    12781 attataaata gaatcaggca gggaatcatt accattaccc cgcctgtttc caccgcctgc
    12841 caagacggga atttcccatt ctgcggcagc aagtctgcct tgccgcaaaa tcaaatctaa
    12901 gggcaacgct gtataaagcg tgtttccctc atacaaaatg gggatcgtga ttccgcaaaa
    12961 gccgctgtct gccgcctatc tcattgtatg gcggtcagcc gcggagatat gctaaaatat
    13021 cctcacactt gtgtttgagc cgaacgaaaa tattctgctc ctccccatcg atctctacgg
    13081 tatcctcttt ctgaatctca atatattgaa tctgggactg atccagtttc tggagcccca
    13141 gatgattttc cgcgtaatcc ttgatgttgc gaatggaagc cttgccctcc agttccgtct
    13201 gcatccgcac gttttcgctg cggaggttgc tcagttccga gttggcatcg gagatggacg
    13261 aatagacttc gttcagctgc atatagctgc tgatgacctg actgaacatc aacagtgcca
    13321 gcacgccggc gataaacacc ttaatggcag caccccgctt ttcggggctg aaccggaaca
    13381 tcaaaaatct ggtggaagta ctctttttgc tgtcagtttc cgcaggtttt tccggttgtt
    13441 tttttgcgtt tcctgccatg aatcagcgac tcctttccag aacccgcagt tttgcactac
    13501 ggctacggct gttctgttcc acttcttccg cactgggtgc aatgggcttt cgggaaacca
    13561 gcttcgcctc cggcgttctg ccgcagacac actgggggaa atccggcggg cagatacagc
    13621 cctgacacca ctgggcaaac tgccgcttga ccatgcggtc ttccagcgaa tggaacgtga
    13681 taaccgccag tctgccgccc ggcttcagca cggaaaatgc cgctttcagc ccgttttcca
    13741 gatgttcgaa ctccccgttt accgcaatgc ggatcgcctg aaacgacttt ttacagggat
    13801 ttttcatgcg acgcaccttt tgcggcacgc cgcttttgat aagctctgcc agttccagcg
    13861 tggtatgaat gggctgttcg gctctggcac ggacgatatt gcgggcaatg ctgcgggcaa
    13921 aagattcctc cccgtaggta tagagaatac gggaaagttc ctcctccgaa gccgtattga
    13981 ccagatcttc cgccgtcatg ccggtctggc tcatacgcat atccaggggt gcatcgtgat
    14041 gataggaaaa accgcgggac tcctcgtcca gctggtggga agaaactccc aaatccagca
    14101 gcacgccgtc caccggcacc tcgcccatct ccgtcagcac ctctgccatt tcatcataat
    14161 tgtggtgcac caccgttgcc ggataaggtg ccagccgctg cgttgcaact tccacagcat
    14221 cagggtcgcg gtcaaaaccg atcagttttc cgcctgccgt cagacgcttt gcaatttccg
    14281 tggaatgtcc ggctccgcct acggttccgt ccacataaat gccgtccgga cgaattgcca
    14341 gcccctccag cacctcatgc agcagcacgg gaatatgtac aaaatccata agcttctcct
    14401 aaaatccgat ttcttctaac gtttgcagca tttccctggg atcccatgcg gcgttcaatg
    14461 cttcccaccg agcccgatcc cagatctctg ccttggtatt ggcaccaatg actacaacat
    14521 ctttttccag tccggcgaac tgccgcagat cctgcggaat cagcacacgc ccctgcttat
    14581 ccggtgtcag aacaccggct cccgaaaaga gccaccgccg aagctgtccg cccttggatt
    14641 ccggaatagc agccactttt gcggcaaggc gttcccactc cgcagcggag cagaccgtca
    14701 gacagagctg atccagcccc tttgtcacat aaaatgttgt gcccagaatc tcccgcagtt
    14761 tcgtggggaa gttcatgcgt ccctttgcgt caatattatg ataatatgta cccatgaggg
    14821 aagccacaaa cgccgccgcc tttctgcaag aagctgcggt tttccgtgaa aaacgttcca
    14881 ctttcaggga atcttcccca cttttcaccg ctagttatta ttataccgta tctaacccga
    14941 aattgcaagg cgacagcggc agaaaacgaa aaaaatatgc cagaaaaaat gtgctttttt
    15001 tatacacctt gcacaaacaa aaaaggacga gccgaagccc gtcctctttc agagaaaccg
    15061 caaaaatgcg attcaattat tcgttaattg ctgtaacgac accagaacct acggttctgc
    15121 caccctcacg gatagcgaaa cgcagacctt cttcgatagc gatcggctga atcagctcaa
    15181 cgtccatttc aacgttatcg ccaggtgtac acatttcaac gtctgccggc agagtgatga
    15241 taccggttac gtcagtagtt ctgaaataga actgcggtct gtagttgttg aagaacggtg
    15301 tatgacggcc gccttcttcc ttcttcagaa cgtaaacctg acccttgaac ttggtgtgcg
    15361 ggtggataga acccggcttg cacagtacct gaccacgttc aacctggtca cgggtgatac
    15421 cacgcagcag tgcaccaacg ttatcgccag cttctgcgaa gtccagagac ttacggaaca
    15481 tttccagacc ggtaacaacg gtgttcagct tttcttcaga cagaccaacg atttcaaccg
    15541 gttcgttaac gttcagcttt ccgcgttcta cacgaccggt aacaacggta ccacggccag
    15601 aaatggtcat agtatcttcg atcggcatca ggaacggctt tgcatcgtca cgttccggag
    15661 tcggaatgta ctcgtcaaca gcgtccatca gttcgatgat cggcttgtaa gccggatcgc
    15721 tcagatcgtc cggagcttcc agagcagcca gagcagaacc aacgatgatc ggagtgtcat
    15781 cgcccgggaa gtcgtactcg ttcagagttt cacgaacgtc catttcaacc agttccagca
    15841 gttccggatc gtcaacctgg tcagccttgt tcaggaatac aacgattgcc ggtacgccta
    15901 cctgacgagc cagcaggatg tgctccttgg tctgagccat cgggccgtca gaagcagcaa
    15961 ctaccagaat tgcaccgtcc atctgagctg caccagtgat catgttctta acatagtcag
    16021 catgtcccgg gcagtcaacg tgagcgtagt gacgcttgtc agtttcatac tcaacgtgag
    16081 cggtgttgat tgtgatacca cgttctcttt cttccggagc cttatcgatc atatcgtaag
    16141 cttcgtactg agcctgaccc ttcagagcca gagtctttgt gatagcagca gtcagagtgg
    16201 tcttaccgtg gtcaacgtga ccaatggtac caatgtttac atgcggtttg gttctttcaa
    16261 atttagcctt tgccattgga ataatcctcc tcaataatga attttaattc agcgtaatgc
    16321 acttacgaga agtgttacac atattgtacc agatttcaaa gcaaattgca agtctttttt
    16381 gaaaaaaaca gagaattttt caaaaaagat tacttcttgc ggtctgtcat gatcttttca
    16441 gcaatattct tcggaacttc ctgatagctg tggggttcca tagcatactg accacggccc
    16501 tgtgtgttgg aacgcaggtc gctggtgtag ccgaacattt cagacagcgg caccagagcg
    16561 tctacctgta cagagcccgg acgagcttcc tgaccctgga tctgtccacg acgagagctc
    16621 aggtcaccga taacggtacc agtgtaatcg tccggaacag taacggaaac cttcatgatc
    16681 ggttccatca gaatcggctg tgcctgcttc attgcttcct tgaaagcgat agagccggcg
    16741 atcttaaatg ccatttcaga ggagtcgact tcgtgataag atccatcgta cagttcaacc
    16801 tttacgtcta caacttcata gccagccaga ataccagcct gcattgcacc gcggatacca
    16861 gcgtcaacag ccggaatgta ttccttcgga attgcaccgc cgacaacctt gttgatgaac
    16921 tcatagccct taccggattc gttcggagcc acacggatct taacgtgacc gtactgaccg
    16981 gaaccaccgg actgcttctt gtacttgtag tccacgtcag cagtaccctt gatggtttcc
    17041 ttataggata cctgcggagc accaacgttt gcttctacct tgaattcgcg aagcagacgg
    17101 tctacgataa tgtccagatg cagttcgccc atgccggcga taatggtctg accagtttcc
    17161 tcgtctgtcc aggtacggaa ggtcgggtct tcttctgcca gctttgccag agcgataccc
    17221 atcttgtcct gaccagcctt ggtcttcggc tcgattgcca actggataac cggttccggg
    17281 aattccatgg attccagaat aaccggagcc ttttcgtcgc acagagtatc accggtggtg
    17341 gtgttcttca gaccaacaac ggcagcaata tcgcctgcgt atacagtttc gatatccttt
    17401 ctgtggttgg agtgcatctg tacgatacgt cccatacgct ccttgttgtc cttggtagcg
    17461 ttcaggacag ttgcaccagc attgacagta ccggaatata cacggaagaa gcacagctta
    17521 ccaacgaacg ggtcagttgc gatcttgaat gccagagcag cgaacggttc actgtcggaa
    17581 gaatgacgga acacttcctc gccggtttcc ggatctacgc ccttgatgga cggtacgtcc
    17641 agcggggacg gcatgtaatc tacgatagcg tccagcagct tctgtacgcc cttgttacga
    17701 taagaagtac cgcaggtaac cggaaccatc ttgtttgcga tggtggactt acggatgcat
    17761 gccttgatct cgtcgatggt aatttcttct tcgttaaaga acttttccat cagagcatcg
    17821 tcctgctctg caacatgttc catcagttcc tgatgatatt cttcagcctt ttccttcagg
    17881 tcatccggaa tttcttctac gcggatgtcc ttgcccagat cgtcatagta aacataagcc
    17941 ttcatctcaa ccaggtcaac gatgcccttg aactcgtcct cggcaccgat cggcagctgg
    18001 atcggaacag cattacactt caggcgatcc ttcatcatgt tgataacgtt gtagaagtca
    18061 gcacccatga tgtccatttt gttgacataa accatacgcg gaacctgata cttatcagcc
    18121 tgacgccata cagtttcagt ctgcggttct acaccgccct ttgcacacag tacggtaaca
    18181 gaaccgtcca gtacacgcag agaacgctca acttctacag tgaagtccac gtgtcccgga
    18241 gtgtcgatga tgttgattct gtgaccagcc cagtgagcag ttgttgcagc ggaagtgatt
    18301 gtgataccac gttcctgttc ctgttccatc cagtccatag ttgcggcacc ctcgtgggtt
    18361 tcaccgatct tatggttgat accggtatag aacaggatac gttctgttgt tgttgtttta
    18421 ccggcatcga tgtgagccat gataccgata tttctggttt tttccaggga acaatctcta
    18481 gccatagatg atcctcctta tacagcagca caaagtgctt accagcggta gtgtgcaaac
    18541 gccttgtttg cttctgccat cttgtgtgta tcgtcacgct tcttgcaggc accgccggtg
    18601 ttgttcagtg catcgagaat ctctcctgcc agacgctcct tcatggtctt ttcgttccgc
    18661 agtctggaat acttggtgat ccaacgcagt cccagagtct gacggcgttc cggacgaact
    18721 tccatcggaa cctgatatgt ggcaccgccc atacggcgag ccttaacttc cagaacaggc
    18781 attacgttgt ccatagcctc ctggaacatt tccagtgcgt ccttgccggt cttttcagcg
    18841 acaatatcaa atgcaccgta aacaatcttc tgggcaacac ccttcttgcc gtctaccatt
    18901 acattgttga taagacgggt tacgagcttg gagttgtaaa tcggatcctc cagcacgtca
    18961 cgttttgcga ttttacctct tcttggcatt ttcgcttccc tccttcacaa taattcatga
    19021 attcacaggt actcaatcgg gcggaatttc cgccgaccgt agggccaatg gagaggaata
    19081 cgccggcacc cgaactggtg ccaatctata ttcaaatctc cacagttccc gtggtgatct
    19141 cagttttcct gttacgccgg agcagttttc acgctgcccc tgatttttgt cggtaagttg
    19201 cagatcgaaa aaagatctta cttcttgcca gccttcggtc ttttcgcacc gtacttggaa
    19261 cgagcctgca ttctgtttgc aacgccctgt gcgtccagag taccgcggat gatgtggtaa
    19321 cgtacaccag gcaggtcctt tacacggccg ccgcggatca gaaccacgct gtgctcctgc
    19381 aggttgtgac cgatacccgg aatgtaagaa gttacctcat aaccgtttgt cagctttact
    19441 ctggcaatct ttctcatagc ggagttcggc ttcttcggtg tagcagtacg aactgcggtg
    19501 cacacgcctc tcttctgcgg agaactctga tcggtagtaa tcttcttctt ggcattatag
    19561 cccttctgca gtgccggtgc ggtagacttg tactgctgta cctttcttcc cttccgaacc
    19621 aactgattaa aagtaggcat aattttcctc ctttgcaaaa aaatgaattt gccgcagcag
    19681 acaagtctgc cgcatgcttt tctattttat cacattcagt ttgcgttgtc aacgctttta
    19741 cagaattttt acaaaatgca ggcttgtctt gcggaaacac ggcgtactgt gtcacggaaa
    19801 tcaattttca tgatagcctc ccctgaaagg ggagggggac cgccgttagg cggtggagag
    19861 gttttttcgt aaaatctttt tatgacgttt ccggcataag tggaaacccc tcagtcagtg
    19921 ctacgcactg ccagctcccc
//
LOCUS       DAWBGR010000021        19925 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_939_length_19925_cov_4.241520, whole genome shotgun sequence.
ACCESSION   DAWBGR010000021 DAWBGR010000000
VERSION     DAWBGR010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19925)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19925)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19925
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_939_length_19925_cov_4.241520"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         5169 a         5230 c         4455 g         5071 t
ORIGIN      
        1 caattgtgca agattggcgg cagctttgct gaagaaatac aatcttgata ttaaccacct
       61 ctttacccac acccactggc tcaatgtcag ggacgggaaa tcgggtactg tggattatct
      121 caatactgca agaaatcctt acaagatgtg tcctgcgtac attctgcctc attgggtagc
      181 ttttaaagct aaggtacaat cttatcttaa ttctggttct acaccggcaa ctcctacacc
      241 tgcaaaacag ctttatcgag taagaaaaac atgggctgat gctaagtcgc agattggggc
      301 atattcctca ttggaaaatg caaaaaaagc ctgcaagacg ggatattctg tctttgatgc
      361 aaatgggaat gttgttttca gcaatggaaa gtcctatgca aaaggtgcaa aggtaacact
      421 caaaaatacc gcactttacg catccgcagc tgcaaaaaca ggtgtgaagc gttccggtac
      481 gttctatctg tacgacggca aggtcatcag cggaagaatg agaattacaa acagtcccaa
      541 aaactgcggt aatacgccga ttggcaagta tgtgactggt tgggtaaata aatctgacat
      601 ctgattatct caaaattttt tctatcccgg agttgttaaa actgctccgg gataattttt
      661 atcacaagaa ctgatttttt tccgaagtat gaatgggggg ggcattttaa tgaaagaaaa
      721 tcatgatgaa ctcggctatt ttatggtgca gactctcacc gaaaaaatgc ttgaaaaagg
      781 actgatttcc tttgacgaat atgacaaatt aaccgcatta aaccgccaaa ctttctcacc
      841 cctttacgcc gacttattgc cgaaaagact tgatatatcg ccaaatttag tttaatatgt
      901 tcataccgaa aggagggaaa gtcaatggct gttacaaaaa ttgacgcaag accgcagccg
      961 aaaacgccta agaaaaaagt ggctgcctac gcaagagtat cgaccgataa ccttgaccag
     1021 aaagagagcc ttgacgcgca gaaaaagcat tatgaaatgt acatcaaagc ccattcaact
     1081 tgggagtttg cgggacttta ctttgacgaa ggcgtttccg gaacaaaatc tgaaacgaga
     1141 aacggtttgc agaatatgct ccgtgattgc aatgcaggaa aaatcaatta tattttgacg
     1201 aaatcagtca gtcgttttgc ccgaaatacc gtggattgtc tgcaaatcgc acgtaatttg
     1261 aaagacaaag gcattttcat ttacttcgag aaagagcata tagacacagg aaatatggac
     1321 agcgagttga tgctttccat tttcagcagt tttgcggcgg aggagtctat ttcgatttcc
     1381 gaaaatgaaa aatgggctat ccgaaagcgt tttcaggacg gtacatacaa gcaatcctat
     1441 ttaccctatg gatatgtgaa aaacgaaaat ggtgaaatga tacccgataa acaggaagct
     1501 gaaattgtcc gctggatttt caatctcgcc ataaacggac acagcacaca ttccatttgc
     1561 aagctgctcc gtgaaagaaa cgtgcctaca agaaagggcg gaaaatggag ttcttccacc
     1621 gttcgtgata tgctgaaaaa tgagaagtac acgggtgacg ctctttttca gaaaacctac
     1681 acagacggca gtttccgcag acatgaaacc aaaaattcgt caaaacaaat cttcattgag
     1741 aatcatcacg aggctataat aagccgtgag atttttgaaa ctgcaaaccg acttttagag
     1801 cagcgagctg ctgaaaaagg catcctcaaa gggcaaggaa agtatcaagc ccgatatgct
     1861 ttttcgggaa ttatcgtctg cggagaatgc ggcggaactt ttaagcgaag aatccacgac
     1921 cacggctcgg aaatagcatg ggcttgtacc acgcatattg agaatatttc aaaatgctct
     1981 atgaaatata tccgtgatga tgcattgaaa gcggctgtta cgaccatgat caacaagctg
     2041 atttttggca gaaaaataat actttttcct tatctggcag agctgaaaag atgcaatgcc
     2101 aattcggaag aaatcaaact tttaaaggca gaactcatca aagaagccga gaaaattgac
     2161 gttctaaggc atctcttggc agataaaatc atcaagcctg caattttcat tcaggatcag
     2221 aacgcaagta acaagcgttc agaggagctt cgcaggcaga tcacgcagtt gagtcagatt
     2281 gataccggaa attccgaaat ggttgcagaa accgagaaac tcttgcattt ccttgacagt
     2341 gcagaaatgc agacggagtt taaggacttt tttgcaacgg attttattga gaaaataatc
     2401 gtatatgatc gccacagcat cggcattcag ctgaaatgcg gactgacgtt aaaggaggca
     2461 ctgctatgag ccacattccc tacggctacg aagtaaaaga cggaaaaatt tttattgtag
     2521 aagaagccac tgataaggta cgcctgattt ttcagaatta tgtatcgggt atgagtttga
     2581 cggaatccgc aaaaacagcc ggacatccaa ttacccacag tatggtaaag cagcttttac
     2641 agagaaaatg ttacattggt aacagtttct atccgaaaat cgtggatgaa aatttattcc
     2701 gtcgagttca gatagaactg caaaaacgtc agaaatctac gggaaagaca agacgaaaag
     2761 tgccggtaat acgcacaaat tttgtgcttt ctgatgaaag agaaccgcaa gaaaatttta
     2821 caaagcaggc agaatattta tacagtctga taggagtgaa ataaatggtt agaacaatcc
     2881 caccaaaacc gcagatcggc aacaacgccg cacggcatac agaaacggtc aaaaaactgc
     2941 gtgttgccgc ctatgtcaga gtttctacgg aaaacgagga acagcagacc agtttcgagg
     3001 cacagtatga gcattattat gactacatca cgaatcaccc tgcatgggaa tttgcaggca
     3061 tatatgccga tgacggtata tcagcagttt cgaccaaata ccgtgagcaa ttcaacaaaa
     3121 tgattcagga ttgtatggac ggcaaaattg actatgttgt cacaaaaagc atatcacgtt
     3181 ttgcccgaaa taccgtggat tgtttgaatt ttgtccgcaa gctgaaggac aagaatatcc
     3241 ctgtattttt cgagaaagag ggcattgaat ccaccgacca gcgcggtgaa attatgctga
     3301 cgattttggc gtcgcttgca caacaggagt cggaatcgct cagtcaaaac gtgaaaatcg
     3361 gtatgcaata tcgctttcag caagggaagg ttatggtgaa ttgtgcctgt tttctgggct
     3421 atgataaaga cgctgacgga aagctggtga taaatcctga acaggcagaa acggtcaagc
     3481 gaatctttcg tgaatatctt gagggtgcaa gctgtatcaa aattgccaag ggcttggagc
     3541 gtgacggcat tctgacgggc aggggaaaga cacgctggta tgacagcacg gtcaaaaaga
     3601 tattggaaaa tgaaaaatac atgggtgacg cattgctgca aaagacctat actacggact
     3661 ttttgcagaa aaagcgagtg aagaatacgg gcatcatgcc gcagtattat gttgacgatg
     3721 atcaccccgc aatcattccg aaagagctgt tccttgcggt gcaggaggaa atggcaagac
     3781 gtggctcagt cgaggactgc aaaggcagaa agcgcagttt ttcagcgaat cactgcttta
     3841 caaggctgat tgtctgcggt gaatgtggag atcagttcaa cagaattcac tggaataacc
     3901 gtggtaaaaa atccattgtg tggcgatgtg cgacacggct cggcaaaaag ggagaatgca
     3961 acgctaggac ggtaaatgaa gaagccttga aagatgcctt tgtagctgcg cttaatcaga
     4021 ttcttgccgg aaaacaggaa tttcttctcc aattgaaaaa gaacattgaa tgcgttctga
     4081 caggcggtaa ttccgaaagt acggcagaaa ttgatgcaaa acttgcagat cttgaacgcc
     4141 agcttgtcgc aaaagccgag gagcatgagg actacacaga catagcaagc gagatttatg
     4201 ctctccgcaa gcagagagat aagcttacga tgagcgaaaa tgcgagaaat gactatctaa
     4261 aacggattga ggaacttgaa tctttcattg atgagcagcc gacaaaaata aacaactttg
     4321 aggaatcatt ggtaaagcgt atgctgaaaa aagtcacggt ttttggggat aaattggagt
     4381 ttgaattttt gtcgggagta attgttgaag taaatatgtg aaataagcga acaccctcgc
     4441 tgctgtcgtg gaaatacgat agtggcgagg gtgttttgtt atttattcat catcttcaaa
     4501 atcgtcataa tcaggatatt gctcaggagg ttcaccaaca actcttataa gctccgcatc
     4561 tttagtatcc tcataaacaa tacgaaggac tttgcaattg aactcccagt catcaccaaa
     4621 atcaaagaca aatttaaatg cttttccctt tgtaagtcca agctgatgaa gcttaaaatc
     4681 gcaggtgtga cggtacattt catcttcatc gacttctgcc atgtagtagc aatcataatt
     4741 actccaagcc ttattgtcca tgaaaaaagc gtgagcgtgg tcattatcga aatcaaatgc
     4801 ccacaaaata atatctgcca gcgtttctaa atcttcattc actgaaacct gaatatggcg
     4861 gtaacagcct ttcataacgg aaacactaag tataattgat tgtttaactc ttttacttgc
     4921 cattttaatc ttcctttctt gtgacaacat ttccacgcac tcaaccctac ccgaaataca
     4981 acattttcaa gcactcaacc tgcgagtgct gtattttgat agtgcttaga tttgctgtca
     5041 gatatgtttc cgtgaactga cttacagcat atgttccgag tgcgtagatt tgatgatatt
     5101 gcaaaaacca cgcaaaatag gcattttctc agttattttt gcgtaattct tgacatcaat
     5161 accacgcact caacgtgccc cgtccccggg aacaaatcca ccggctgcac ctgttccacc
     5221 gcatatccca gttttctcaa cgccgcacaa tctctcgccg cagtcgtagc gttgcaggag
     5281 atcatcacaa ttcgctcagg ctgcatctgt accatagcac gaatcgctgt ttcatcgcag
     5341 ccttttctgg gcggatcaac cacgatcaca tcgggagcag tcccctctgc tgccagctgt
     5401 tccgcaatgc tgcctgcatc gccgcaaagg aatttcgcat tttccacgcc ggctctcgca
     5461 gcatttttct tggcattttc taccgcttct ggtacaattt ccacgccaac caattccgac
     5521 accgcatctg ccatggacag cccaatggtt ccggcaccac agtacaaatc cagcaatcgt
     5581 tcctttcccg tcagctgtgc aaaagccttt gctgcaccat aaagccgttc cgcctgtatc
     5641 gtgttcacct gatagaacga ctccggcgaa aggtctacag cattgccgca catggtgtct
     5701 gtaatcgtat ctttccccca cagcgtttct gtagaatctc ccagaatcac gttggttttc
     5761 ttcgaattac agttcatgac aatgctctgg atctgcggaa actgctccgt cagctgctgc
     5821 accagaggca tcagcttttt ccgaattggc tttcgcacaa tgaagcagat catcacctcg
     5881 ccggaatgct gcccgcaccg caaatacaag tgccgcagtt ccccttttcc gctggcttct
     5941 tcatacaccg gaatccgatt ttttctcaca aactccagca cgacaccagc aatttcccca
     6001 aacagttccg gctgtagcaa gcagtctttc accggaatga cccgatgact tcttctggca
     6061 aaaaagccca gaaccggttc tcctgccttg tctattgcaa aaggatactg tgccttatta
     6121 cggtatctgg tgcgggattc tgcccccaga atcggcagaa atgcaggcga cagtttcccg
     6181 attcgtgtaa aagcgtcctg cactgccttt tccttgatac gcagttctgc cgcataagtg
     6241 atatgctgaa acagacagcc cccacactgc gacaacgggc aggcaggcga aatacgatcc
     6301 gccgaaggtt ccagcaattc ttccacgatg ccaaaaccat agcttttcag cactttcaca
     6361 attcgcaccc gaatctgatc tcctactgct gtatgcggca caaatactgc catgttgtcc
     6421 acccgacaga caccgctgcc ctctgtggtc atatcaatga tttctgtttc aaaaatgtcg
     6481 ttctttttca actctccacc gcctgatata gaagcaacac cgcacaaaat tgcctttaca
     6541 tttgtgcgat gctgcccgat tatttcattt cttccagata tttcgccgga actgcttctg
     6601 tcagccagat accatttacc gaacggtaaa aagcatagcc gtcctctgcc atttttccag
     6661 tctgcacccg cagcagcact ggctttccgt gacgtgctcc tactgtctgt gcaatttccg
     6721 gcgtggcaga cagatgcaca tacagccggc tcattcgccg cagtccctcc tgaaaaatcg
     6781 ccggcaaaaa ccgttctgcc gttccatgcc acaagaattc cggcggcagc acctgttcca
     6841 gttccacatc tactggaatc gagtgtccct gattacaacg aatcagctgc ccgtctgcac
     6901 tgaacgaata gcgacttttc acatctgtac gaacaatttc ttccagctgt tcccgacaca
     6961 aagggcaccg ttctgaaata ccggcaagga gttcgtccac atctgcccaa ccgtgctcgt
     7021 ccagcgtgat gccaattttc tccggatgat gccgcaaaat aaaacaaaga tattttccga
     7081 ttttatcaaa atccatacat tcttctttcc atcttctttc cattgaagct cagaagatcg
     7141 tttcataatc ttcttcctcc tgccatgcac ccataccctc acaaggtcct acatagagaa
     7201 ttttatctcc acgaatgatc cagtccagtg catgctctgg ttcccagacg caggcactgg
     7261 aaatgctata tccgcaaaca gcttcctccg gcggttcaat gtacatttca ggcgtggtca
     7321 aaaatgccag cacttctctc ccgtgcagtt tctccggaat ttcacgctga atgtcttcta
     7381 caatgtccgg atagacatct tcaaattctt tccattcctt ccgcataaac tcacaatacg
     7441 caattgccat ctcgcaaaac ttgtcgatca tctggtcaga gagatgatta aaatgctcca
     7501 cgcactgctc tgcatatgcc atatcgactt ccggatcaat gtgaacaggg attgcacgct
     7561 gaaataaaac agatgcaaag gttccgttcc ggaaatattc atcctggtcc tgtatacaaa
     7621 tcaaactcat ttttccgcct cttgttcccg aatccgcttg cagatctccc gcacctgttc
     7681 ctttttctgc atcagctgat cgaactggcg gatatactca taggggaatt tataattgtg
     7741 aattcgtgta attctgccgc agggttccac cagcttggta gaagctccgc accctgctcc
     7801 aaaaatggac tggctctcgt ccatgatcag aatattgtac agcgactctt tccctgcacg
     7861 ggcatagccc acattctcct gattgtcgat cgtattcttc tgccggtaca tataatatgg
     7921 ggcatagccg tgctccggca gccgccgcat actctcctgc accatttgtt ccaccggatt
     7981 atgtacctgc tgctcaccct ccgcaaaaag attcgccgcc cgtttcagcg tcagagcgtg
     8041 caccgtgatg ctgtccggat ccaatgccag aatctgttcc agcgtgtcat gaaatccttc
     8101 cggcgtatcg tccggcaagc cggcgatcag atccatgttg atgttctcga atccggcagc
     8161 ccgtgccatg cggaatgcct ccaccgtttg tgccgtagtg tgctttctgc cgatcgtttc
     8221 cagcacgtga tcctgcatag tctgagggtt gacagaaatg cggtctacgc cgaaacgccg
     8281 cagtgtttcc agcttttccg aggtaatggt gtccggtcgt cctgcctcga cacaatattc
     8341 ccgcaaatgc gaaagatcaa agttttcctg caccgccgtc agcacctgtt ccaactgtgc
     8401 cgccgtcagc gaagtgggcg taccaccgcc aaagtagacc gtatccacca gcaattcctg
     8461 ttcccgtacc agctgtcccc aaattttcag ttcctcgcac agcttttcca gatatgccgg
     8521 aatcagattc tgagcctgtg caatggaatg ggacacaaaa gaacaatagc tgcaccgtgt
     8581 aggacaaaac gggatcgaca catacaggct gatgccgtgc ttcggtgttt tttccagaag
     8641 cggctgctgc accattgctg tatcatagac cagcgacagc ttttccggag cgatccagta
     8701 cttttcgtgt aatttctcac agacttccgc cttggaaagc ccctgtccca gcaaaggctg
     8761 tacctttttc accggacgaa ttcccgtcag cagtccccat gcagggtgca gatcagtgat
     8821 ctcttccaac agccggaaca gcatggcact gaaccggtat tcgcaaagct gtttttccgt
     8881 ttccggagaa caggctggaa actgttgttc ctgctccatc acctgccccc gatactgtac
     8941 ctgtacccaa aagcggctct gttcgcccaa ttgttccaga atcagaacag ccccctcctc
     9001 tgcacctgcc ggcagctgtg tcgtgtcgta gagaaaggta aacttccgca tcggcagaaa
     9061 gattttgcag atacattcga tttcgtattt cagcgtgtgt ccggaaaatg ccaccggaaa
     9121 cggctttatc agattggttt caacattcga catgaatcaa agtcctcgca tggttggatt
     9181 gtgtgctttt tcaaaggaaa gcgtcgattc ctcattgtgc cccggcagca cccgatactc
     9241 cccttccaga gctttcagtt tccggaacga ttccagcatc ttggcatcgt cgccgtccgg
     9301 aaaatccgtt ctgccccgcg acatgcggaa cagcgtatca ccggtataca gcacatcgcc
     9361 ggatcgccag caaacgcttc cggaagtatg ccccggcgta tgcagcaccg tgaaagtact
     9421 gtcacaaaat tgcagcgtgt cgccgtcctc gacagtttcc cacgcctgca ccggctggaa
     9481 cggctgtgtg gagatccagt ctcccagatt ggcatgaccg tccgtcagca tatgggcatc
     9541 cagcgtgtgc acataaacgg gaatgtcata tttctcccgc acctgctcca cgccggcaat
     9601 gtggtcatag tgtccgtgag tcagcagaat cgccgccggt tccagtccca gcattcccag
     9661 ccggcgaaac agcaccggtg catctccgcc aatatccacg acaacacatt ttccatttcc
     9721 cactggctgc acatagcagt ttgaagccag ttcacccagc attatttttg taatttccat
     9781 tgcatgccac ctcacttttt tgcacgttct accgagatca caccgcgaat cttccgcagc
     9841 ttatcgatga tcgcctgtaa ctggtctgtt ccggcaacct gtacacttgc ctcaaaaatc
     9901 gcattgccgt tccgcaggtt acgggaggag gagtgcacga tcagaatatg ggactccgag
     9961 agaatcttcg taatatcgaa taccagtccc acacggtctg tggcaatgac ttcaatgctg
    10021 gtggggatca gcgtggaagt gttattggtc cactgcaccg gcagccaccg agccatttcc
    10081 tccggatcat tccgtttcaa agctgcctga taattgacac agtttttggt atgcaccgaa
    10141 atgccatgtc cgcgggtaat gaatcccaca atgtcatctc ccggcagcgg gttacagcac
    10201 tgggcgaact tgaaaacgca gtcgtcaatg cgatccagta caatacagtc cttgccgctt
    10261 gcagtttccg gcggcactac cggtgctacc ggattggctt cttcctcctc tggcccgtgg
    10321 gatttcacat atagttcttt cagacgcggc atgatcttgg acagcattac accgccgtaa
    10381 ccgatagagg catagaaatc gtccagtgta tcacagttgt gccgtttcag atcatctgcc
    10441 agaaaagctt cccattccga ttccggcaaa ttgatatgac agcgtttgaa ttcctgttcc
    10501 agacttgcct tgccggtagt gatattctcc tcccgacatt cctttttgaa ccacgaacgg
    10561 attttcgatt ttgcttcgtt ggtcttgaca attcccagcc atgcccgatt cggccccttg
    10621 ttggggtctt tgctggtgag gacttcacaa atttctccgg tttgcagctg ataatccagc
    10681 ggcaccattt taccgtccac cttgccgcca atggtcttgt gtccgatctc cgtatggatc
    10741 cgataggcaa agtcaattac cgttgcaccc accggcaggg aaatggaatc tccgcggggt
    10801 gtcatgacca caatatcttc cggtgacagg tcgttcttga tcgcacgaac aatttcttct
    10861 acatcgtcag aagtctgctg tgcctcaatg accttgcgaa cccatgcaag gcgttcttcc
    10921 attttgtcct tgccctgaat gccctctttg tatttccagt gggcagcgat gccatattcc
    10981 gcagaaacgt gcatatccca tgtccgaatc tgaatttcaa agggaatact ctcccgtccc
    11041 agaactgtgg tgtgcaggga ctgatacata ttcgacttcg gcgtggaaat atagtccttg
    11101 aaccggttcg gcagcgggcg gaacatatca tgcacaatac ccagcaccat atagcactct
    11161 gccacagtag aaacgatcac acgaacagca tacttatcat aaatctcgtc catgctttta
    11221 tgattgacat aaactttttt gtaaatgcca tagatgctct tgacacggcc ttcaataata
    11281 ggctccttgg caaagtgttc tttttccagc cgcttgcgga ttcgctcctt aatcgagtcg
    11341 atgaactttt cccgttcgtc cttcttgatc tccatgatgt gttcgatctc tgaatatgca
    11401 taggagtcca gatagtagaa cgataagtcc tccaattctt ccttgactgt acggattccc
    11461 agacggtgtg cgatcggagc atacacgttc atggtttcca gtgcgatgcg acgctgtttt
    11521 tccggcggga gggatttcag tgtccgcata ttgtgaatgc ggtctgccag cttgatgatc
    11581 atcacgcgaa tgtcatggga cattgccagc aggatcttcc gaacattttc tgcctgttgc
    11641 tgctctttgt caaatatggg aattcggttc agcttggtca caccgtccac cagcattgcc
    11701 acgtcctgcc cgaatttctt gcgaatatcg tccagcgtat aattcgtatc ctctactaca
    11761 tcgtgcaaca gtcccacaca aatagtatcg gtatccatgc ccagatccag cagaatatct
    11821 gcaacagcca gcgggtgaat gatatatggt tctccggaag accgtttttg atcagcatgg
    11881 gctttttctg ccagctggta cgccgagaga attttactta agtcatactg cttatctgtc
    11941 gccagaatct tcttggacag gtctgcaatg ctaacttcat gctgcggaat ctgcggtctg
    12001 tcctgtgcag acggtgcagc agatcgggta tccccatttg tgttctcttg tgtcatacaa
    12061 catccactcc tcttttctgt aatccctgca gcaggactga ctgctgcaga tccacctttg
    12121 ccgaaaccgg tttccggtgc acctgccgtg tccaaagatc ctgctgtacc aatcccaatt
    12181 ctgaaaaaat atccaatgcc agacggaact tgcaatagtt catctgcggc agatcctgca
    12241 tgccgccgaa cagtccgtca aaagtggtat ctgtctgcgg gatccgctgg tagatctgta
    12301 ccagcacgtc ccgcgtcggt gtgatcgccg gataaaaact gggctgcaat tcctcatgcc
    12361 ggcaatattg ttcgtatgta ttttccgcgg caaaatactt tgcctgtttc agaccgcttt
    12421 tccgatagtc cttgacaatc acagaaagct gcggtctgcc ctgaaaactg ccgacctcta
    12481 tgttgatcag aaaatcgcac acatcgccag ttttcagcat cacttcttcc ggcctagtcc
    12541 ggaacagcag cagatccatt gccatgccgc cgtaggtgat tttcagcttg gagtgcgttc
    12601 cgccggacag tggcaggaca ctttgcagaa ccgcgtggca aatggcaaat accggcaccg
    12661 gattgccttc gccaaacggt gccagtgcct ccagtccggt gatgttctcc atggtgatct
    12721 cctgcggcat cagcagctta tccgcctcca gtgtcagaac cggcatgacc ggattggact
    12781 ccttggcgta ttgctgtacc gcctgccgga atcgctctac atgctcggtc ggcaaagaaa
    12841 atccgcctgc accggggtga ccgccatagc gtgacagcaa ttcccgacac gcgtgcaggc
    12901 agcgaaacgc agaaaacgca ccaaaggaac gcatggagcc gcgggcacgg tcgccctcaa
    12961 gggtaatcag aatcgttggc tttccgaact gttcttccag tcgggaagcc gcaatgccga
    13021 tgacaccatg atgccagccc tctccggcaa acaccagcac ccgttctttc agaagccggg
    13081 gattctctgc cgcctgctct gcaatttctt ccagaatgcg aacttcctct gccttgcgtg
    13141 cctgattgcg gcgttccagt tcctctgcca attctgccgc ttcatctggg tcttcacaga
    13201 gaagcagttc cacagccgtt ttgggtgagc cgaaccgtcc ggcagcattg atacgcggtg
    13261 caatgccaaa cgcgactgag gtggaagtga aactctttcc cagcagtccg cttttttcca
    13321 gcagtgccag cagccccaca cgttccgtat tcgccagaag ccgcagcccc agctgtacca
    13381 gatagcggtt ttcgccggat aaattcacca cgtctgcaat cgtcgcaatt gccgccagtt
    13441 cgccgaactg ctccaacgcc attgccgtat cgccgccgtc cagtgccgcc accagtttca
    13501 acgccacacc ggcaccgcac agatcatgga acggagatgt gtcatctgca cggtgtgcat
    13561 ctacaattgc cgccgctttc ggcagcgttt ccaacggctg gtggtggtca gtaatcacca
    13621 gcgtcatgcc cagatccgcg atcagttccg cttccgcaat ggctgtgata ccgttgtcca
    13681 ccgtcacgat cagttccacg ccgtcctcgt gcatggcgtg gacagcgtcc acattcagac
    13741 cgtatccctc gtcccgttcc ggaatgcgat aggtcacatc ggcacccatt tccagcaagt
    13801 aagagtacag gatcaccgtg gcagtcacac cgtcacagtc atagtcgccg tagatgcaga
    13861 tgcggctgcc gtcgtccaga gccgtgttga tgcatgccgc ggctgcctcc atgtccgctg
    13921 tcagaaacgg atccgccagt ccgtcacact gtaaaaacgc cgccgcttcc tgcacattat
    13981 gcataccgcg tgccgccaga acctgtgcac acagcatgga cagatcgctg ctgctgcgaa
    14041 tctgttttgc cgcttcttct gacggctgat gaatctgcca cttcttcatc ttgttcctcc
    14101 tgcttcctga aaccaaatat cgtaccttat attatacccg aaaaagtgac aaattgcaat
    14161 agtgcccgtg aaaaaaatag gagaacctct acggtgcttt cctgtattta acgtcgcgtt
    14221 cacaaaaaga aacagcaaag ccaaagaaat tgtgttatac taaagctata ttcaaatgat
    14281 tcccatatag gcaacattgc atgatgccgc ctataacaga aaggaagttc ctgttatgga
    14341 aattacacca tatacccgca tggtctttta ccatgaaaca gaccgcatgg acatcgtaaa
    14401 ccactccaac tacattcaca tggcagaaga agcccgcgta gactttatgg agaaaatcgg
    14461 catcggctac agccgcatgg aagaacaggg cattatgctg ccggttctgg gcgttacctg
    14521 tgagtacaag cactccctgc acttcggcga acatcttgct gtatactctt acatcaccaa
    14581 gtacaacggc tttaagctgg aactgcggta tgacattgtc tgtatcgaaa ccggacagct
    14641 ctgtgcggtt gccacttctt cccactgctt cacagacacg aagatcaagc cgatgcggat
    14701 tcgggacaaa tttccggaaa tgcatgcctt tttccagaaa gccatgaaag tgacttatcc
    14761 ggaacactga aaaaagctgc tgcaaatgca aaagcccagt gcgtatttct tataacgcac
    14821 cgggcttttt ctcatgtaat ttaggcaata ccgcacaaag agcgtctggc gggctttgtg
    14881 cggtgttgcc ttaaaaaata tcttcataca gctttcgata gagggagatt ttcacgtccc
    14941 gacaggtggg aaatgcctgc cggaattgtt cggtatcgtc ggcaatggtc acatagacaa
    15001 gctgtttctc gccgccgcag gtacacagta gctctccctc cggattcacc agacaactct
    15061 gcccgtcgaa tgtgtaagac tgctgccgcc ccacgcagtt gacacccagc acatagacct
    15121 gattttcgat tgcccttgcc cgcaacagaa tccgccagtg agccgtccgc tgatgcagcc
    15181 agtttgccgg taccagcagc aagcggtttt tctgtgctgc cgcccgaaac acttccggaa
    15241 accgcaggtc ataacagatc agcgttccca cggaaaactc tcccagcaca gcactggtaa
    15301 gcttctcgcc gctgtggtag aaccggtctt ccccgccata ggaaaacggg tgtatcttca
    15361 cataatccga cagcacaccg ccgtttttgt ccagcagagt atagtggttt tcaccttttc
    15421 catgctgctc ctgtgaagtc cagccgaatc cgatcgcaat accgcactgc actgccagct
    15481 gccgcatttt ctcatggatt ttcggggcat atcccgcgat cttctccacc tgcatggaaa
    15541 agcctgtgaa actcatctcc ggaaagacaa tgcagtccgc ctgtgccaat gctgcctgtg
    15601 cgatccacgc ggcggcattc gccagatttt cctccggctg ttcaaacatg atctctgttt
    15661 gtgcaatggc tatcttcatg ttatccccta cttccggtga gaacctgtca gttctttttc
    15721 ccgttcgttc tcctgcggtg cagatgtccg gtctgtttgt ctggactccg gaccgtgata
    15781 aaatacagta tcagaacgtt tccagttctg ccgcggacgg tcatggaatt cggttgcatg
    15841 gggcaccaga tgaatcggat cctccggtgt cacgtttttc ttggcacgat aggaagcgta
    15901 gatacacaaa ccgatataca gggcagtcag cacaaatccg gcaatgatgc cgttgcggaa
    15961 ccacggtgaa ttggggaaat ttttcaaagc ataggcgtta aacttggtaa aggaacggct
    16021 gacatcagac accgccacaa gcggcacctc cgcaatggtt tctccggaaa atttcagttc
    16081 catcgttccc agttcgtccc cttttttcac tggtgccatg acatttttgt ccagatgaat
    16141 aacggtctgt accgcagtaa tatccacact cttgcaccac agcaaaacgc agtctgtctt
    16201 tgggtgcacc agcacatagg agttcccgtc ggagttcatc acttccacct ctgcggtttc
    16261 ctcactgctc tccagcagcg tggtataggc aaaggaacga aatgcccagt tcagcagcga
    16321 tgtggcatcg tccagatgat agtactgcaa ttttccgtcc gcatcttcaa agggtgcgtt
    16381 cagcagaatt accagatatg tgttctcgtc ccgcgttgcc tcggtgatga tgctccgtcc
    16441 ggtcttgctc aggttgccgg tcttgatacc cttggcaccc tcgtaataat aggagctgtc
    16501 ctccagcgtc atggaatttg cctgtgtcca gttccatgcg gaagcgtcag ggtggtttgc
    16561 cggattggca gtggtgggag taaatgcagt ggctgttgca atttcctcaa agccgtccag
    16621 cgagagagcg tagttggtga tcgtcagcat atcccgtgcc gtggtctgtt gtctggtatc
    16681 gtacagtccg gtagggttgg cgaacgtggt agcggtacag ccaatttcag cggcggtgtc
    16741 gttcatcttg gtgacaaatc cgtcaatgct gccgtccccg aactggttcg ccagaatcag
    16801 agaagcttca caggacgaag tcagcatcat ggcatagagg tattcccgca cggtcaatgt
    16861 atcgccgttg tcaatgtccg cataccgcag gtcgtctggt tcgtcatagc tgtacagcgt
    16921 tttatacagt tcatcactgg cagtgatcgt tgtgccgtcc agatcagtgc agttttgcag
    16981 caccacaatg gcagtcatga tctgcaccag atgaccgggc atctgctgct ggtcagcatt
    17041 tttttcatag agtatctgtt gggtatcctg attgtacagg atcgccgcct gactgtggat
    17101 cgttgcgtcc ggtgtaaagg tataagcctg tacagaaagt gcgtttccgc acagcagtac
    17161 cgcggcaaca atgccgatcg ccaatgcggc acgcagtcgt tttatcaaat ggaacaagtg
    17221 cataagatgc aatcctcccg aaaattcttt attcgtgaaa gttttcaaac tccacattct
    17281 tattataaca aaaaaaatcc gattcgaaaa gacttttttc gaaaatttat ttacctttca
    17341 cggaaaatat gataaaataa tctatgtaac tgaaaatcat gcaaaaataa acaccagagc
    17401 atgaaatttg atttcacttt ttaaggaggg cttccttttg gtatatatca taggtgacat
    17461 gcacggagat ctgtcccgtt tcaaaaatcc cattttcaaa aaaatgaaag ccggcgatgt
    17521 tctgatcgtc tgcggcgact tcgggtttat ctgggacggt tcccgtcagg aaaagcaaaa
    17581 tctgaaaaag ctgcgggaaa tgccgtttac cattgccttt gtagacggct gccacgaaaa
    17641 ctttgacatt ctggaacaaa gcttccggac tgtccgctgg cgtggcggca aggcacatct
    17701 cattgccccg aacattttcc acatgatgcg tggcgagatc ttcaccattg acgaccgcac
    17761 ttacttcaca ttcggcggcg gacacagtca ggacttcgag ttccggcaag gtcggaactg
    17821 gtggcagcgg gaacaaccca cgcatgcaga gattcgccgt gccattcaca acctgaacga
    17881 gcacaatgca caggtggact acatcatcac ccacgaaccg ccggcttctc tgaaagactg
    17941 ccttggtgtc gatatgcagc agcgtctgga aattcacacg ttcttcgagg acatcatcaa
    18001 acagtgcagc tatcggaaat ggttcttcgg caagtgccac atggaccgct ttattcctat
    18061 gaagttctat gccatgttcg aggatgtgct gccggtagaa tcggaaagcc gcagacgcag
    18121 ccgaaagcag acggctccgg aagaagattg atcctgcaca caaacagccc gacagtttca
    18181 gcaagagaaa ctgtcgggct gtttaattct tatggcaata ccgcacaaag agcatctgtc
    18241 gcacaatctt tgtgtagtat tgccttatct cttttgggta tctttttgct ttcgggcttt
    18301 tttcacgggc aggcgaatcc acttcccctg cgatttttcc cgtgagaaat gctgcttcac
    18361 gtactgatcc agcatttccc gcaccggcac accgccatgc atgggaaaga agaaccgacg
    18421 tacatttttc ttttgcaggg catcgtgaat gtcctcccgc agttcttcca cccgtaccac
    18481 gatattggtt tctttcgcaa attcgcggat ctttaccatc aggtgtgtgg aatatcctac
    18541 atcgatgaga aaaaccccat agaaaaaacc gatgccgaac gaaaacgcca gattttctgc
    18601 cagccagtgc aacgcattca aaatgtgcgg gtggatcaga aaatagtaca ctgcactgag
    18661 caccatccag aaaaatgtaa acaacggaca gataatgccc tggatattgc cccaacggtc
    18721 agaataatcc cacagcttga ttttcatgcc gcggataaag atcagccctg ccacatattc
    18781 cagtgctgtg attgccaatg ccatgaccac aaagagcagg agctttttca gcaacggatt
    18841 ttccaccggc atcagcggtt ccagcatcgc cagcaggtac agcacacaca aactggagcc
    18901 atacagcggc aaatacggcc ctaccagaaa tcccggattc acccagtggt gtgctgaaaa
    18961 gaaccgacgg aatattacct ccagtcccca gccgaggaca cttccgatca aaaataaaaa
    19021 cgccaatgtc aaaaataaat tcatatcagc ctcccctgca ccgacacaaa acactcacgc
    19081 aatagaacta aggtcataca gtgactgatc tgccaatgcc tgttcaataa tatcatagtg
    19141 ggtctgcaac gctcccatgc ccattttcag atcaccgatc atcaggctct tataaatctt
    19201 ttcatgaatg gttgcctgct tctgccgcag tttttccgtg ctgtctgaca agatcaggtt
    19261 gatctgtgca gaacaaatat cccagatgcc ctgcatcaca cattccatga gccgatttcc
    19321 tgaagccttg atcagccagc ggtgcatttc ttcatcagca cgaatactat ccagtccgtt
    19381 gccgtatcgg aattcgtcca gcaagttttt caattcgtcc agatccagcc gttcccgtct
    19441 ggcaaatacc ataggaaatg ccgacaactc cattgcccgc cgcatctgac acacctcata
    19501 gggtgaaatt ttttgtatca gaaacatcac atgcagcagt tcctccatgc tgtcggacat
    19561 atctttagaa agtgtcatct ccccctcttt ggaacaggac agcacaccgg tcatttccaa
    19621 attctttaga atcggacgca catcccgctt tttgatagac agagcttctg cccattcctg
    19681 ttccgagggc agcacatcgc cggcatgcag ctgtccgttt tgcagccgct tccgcaattc
    19741 ctcgatcaga cacaaataat gatactgttg atttttcatt ttcctccatc tcctgtattc
    19801 agtgtttccc caggtcttac cgcgaaacgc cccatgcatc ccgccttctt tttcttttag
    19861 gaaacactgc ataaagcttg tgcgtaagat gcgtagtcag atttttcggc agattgctca
    19921 gaaat
//
LOCUS       DAWBGR010000022        19195 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_973_length_19195_cov_5.038506, whole genome shotgun sequence.
ACCESSION   DAWBGR010000022 DAWBGR010000000
VERSION     DAWBGR010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19195)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19195)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19195
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_973_length_19195_cov_5.038506"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4961 a         4774 c         5448 g         4012 t
ORIGIN      
        1 agcagattgc gtgcaaagcc gtcaggcggg ctttatgcag cgtttcccta atttttcctg
       61 aaataaaaaa gccgctgtgc ataacgcacg gcggcttttt gtttctgttt attcagtgct
      121 tcctgaagtt ttcttcttgc tgacagtacg ctttctggcg gcgggctttt tcacagccgg
      181 ctgctttttc ggctggattc gcagcatgat gccggagaac ggcggcagtt ccagcgtgaa
      241 ctgcatgccg gtgcgggtaa catcgcctac aattgcctgc ttgctctcct cggtgttgcc
      301 gtgaaaacgt ttccagtcgg tgtgcagcag cggtttcagg ctgccgcagt ccggcagggt
      361 aaaggtgaac gtctgcgtgg cttcgtcgga catgtgcagc agaatcagaa tgcggctgtc
      421 actggcacgg cgttccatgg cgtagatgcc cttggcttcc tgatgacagt ccacccagca
      481 gaagccgtcc cgttcgtagt cgtcctgata cagtgccggt tcggtgagat agagccggtt
      541 cagttccagc atgtaatggt agaatgcatc gtgaatggga tatttcagaa tgtcccagtc
      601 ctgctgctgc tcctcggtcc actcccgcag ctgccccagt tcgctgccca tgaacagcag
      661 ctttttgccg gggtgtgcat acatatataa atagagcagc cgggcctgcg ggaatttctg
      721 ttcgtattcg ccgaacatct tctgcatgat cgtggctttt ccgtggacgt tttcgtcatg
      781 ggaaaggggt agcaggtagt attcgttggg aaagtacatc atggagaagg tcagcttgtg
      841 gtaggtgtcg ctgtcccgcc gtacccacgg atactgccgg aagtagtcca gcgtgtcgtt
      901 catccagccc atgtcccact tataatcaaa gcccagtccg ccgtattcta ccggacaggt
      961 cacgccctca tagttggtgg agtcttctgc gatcagcatg ctgtccggat gcagctggtg
     1021 cagtccctga ttcatgcgtt tcaggaacgt gatgctggcg ggattgatgc cgcggctggc
     1081 atcgccgccc cagtagatca gccggctgac tgcgtccata cgcagcccgt caaagtggaa
     1141 aacagacagc cagtagttgg cacaggactg gagaaaactg cacacttcgc cgcgggcgtg
     1201 gataaagttg cagctgcccc attcgctgta gttgtcctct ttctccggat actcatataa
     1261 tgttgtaccg tcatagtggt tcagggcata gccgtccact gcgaaatgca ccggtacaaa
     1321 gtccagaatc acaccgattc ccgcctgatg acaggtgtcg accagctgca tcagctgatg
     1381 tgctgtgcca tagcgggaag tggggctgaa aaagccggtg ttctggtatc cccaggagca
     1441 gtccgcaggg tgctcactca ggggcatgaa ctcgatatag ttatagcctg cctttttgac
     1501 gtagtccacc agcttcgggg cgatctcctc ataggtgtac cagccgttgg ggttgttcgg
     1561 attggttcgc cacgaaccca gatgcacctc gtagatgttg accggtttct tccggtgatt
     1621 gccacgctgt gccagccatt tcttgtcgtg gaacgtgtag ctggacaggt cgcggataat
     1681 cgaagcgttg ttcggacgca gctccatgcc gaagccatag gggtcgcagt ggtcgatgat
     1741 gtcgccgtcg ggattctgcg gggtgatgcg gtacttgtac atctgtccgg cgacggcttc
     1801 cggtacggtg agctcgtaca cgccgccgtc gtggatccgc tgcatgggta tcggctccca
     1861 gtcgccgccc tcccagatca ggtctacggc tgccgcattg ggagcaaggg tgcggaatac
     1921 aacgccctgt tctgtcacgt gtgccccgaa atacgtgtgg gcatcgaaag cgttgcccag
     1981 aaaaaactga tgaatatcca taaggttggt tcactccttc gattgatata tccattgaaa
     2041 ttgcataagg tttgccgttc cgttgtaatt tttttctcaa agtgcatcga aatctagtat
     2101 tattatacta aatctacatg ctgcattgca agcaccaaat ctgcatatag tgttgcatta
     2161 tagacaaatg tacatatagt gtttgccgga actgcacttt ttgagaaaaa ccgaaaaaaa
     2221 tttcggaaaa ccattgcaat cgaacggaaa atgcggtata ataaaaacag gacaacaccg
     2281 tgcaaagccg atacacggac ttgtgcgggg ttgccggaac agatgaccgt ttctgacaga
     2341 ggcggctgta gttaggctgt acgaatctgt ggatacgggg aacaactgtt gcaccaattt
     2401 ttttaggagg cacttattta tgttagtatc ggcaaaggat atgatggaca aggctagaga
     2461 cggcaagtat gcagtcggtc agttcaacat caacaatctg gaatggacaa aggcaatcct
     2521 gaacactgca cagaagctga attctccggt aattctgggc gtttccggcg gtgccggcaa
     2581 gtacatgacc ggtttcaagg ttgttgcaga tatggtaaag gctatggacg aatctctggg
     2641 tatcaccgtt ccggttgctc tgcatctgga tcacggcacc tacgatcagt gctatgactg
     2701 catcaaggct ggctttactt ccatcatgtt cgacggttct cacttcccga tcgaagaaaa
     2761 tctggcaaag accaaggaac tggttgcagt tgctcataag ctgggtctgt ctatcgaagc
     2821 agaagtcggt gcaatcggcg gcgaagaaga cggcgtaatc ggtagaggcg agtgtgcaga
     2881 tccgcaggaa tgcaagacta tcgctgatct gggcgtagac attctggctg ctggtatcgg
     2941 taatatccac ggcgtatatc cggcaaactg ggaaggtctg agcttcgaaa ctctggaagc
     3001 tgtaaagaag gctgtcggcg acatgccgct ggtactgcac ggcggtactg gtattccgga
     3061 cgaccagatc acaaaggcaa tctctctggg cgttgcaaag atcaacgtaa acactgagtg
     3121 ccagctggta ttcgcagaag ctaccagaaa gtacatcgaa gaaggcaagg atctgaaggg
     3181 caagggcttt gacccgagaa agctgctcct gccgggctac gaagcaatca tggcaaaggt
     3241 agaagaaaag atgaacctgt ttggttctgt caacaaggcg taagaagcct gacacactgc
     3301 acgaatctgc agagaaagga atgtgtttct gatgaaaaag acattacagg taatcctcgc
     3361 tgtactggaa atcgtcacat atactctggg aattgtcgtt tggacaaaga acctgattaa
     3421 tttgacacat tctgatgaga acgaaagcac agctgatgag gcgtaatgct tcgaagaaac
     3481 cggtttgcac ccaccgtggt gtgaaccggt tttttgtgca cgtatcggct tcgggcagaa
     3541 aaaagacgga tatgatgcga aaaacgtaaa tttttttgga aaaaaacgta cttttttctt
     3601 caaaagcctt gattttttcg tccggcagtg ttataataag tgtaatcatg cagcaaaaga
     3661 ccggaacccc accggtcttt cgtttatgta ggcacttttt tcggcgaaac acactgccgg
     3721 aaaaagtgcc caatacgcgg aaaaaaggag cagatgccat gaaaatgaag aagttcggcg
     3781 gaacggaaaa ggtcgtctat gacctcgtaa agccgattgc cgatgaacag ggatattcca
     3841 tctgggacgt gagctttgaa aaagagggtg cctactggta cctgcgggtt ttcatcgacc
     3901 accccgacgg gatcgacatt gaggactgcg agaagatgac acggccggta aatgcggcag
     3961 tggacgccgc ggacccgatc tcgcaggcat atgtgctgga agtcggctct gccggtctgg
     4021 aacgggaact gaaccagccg cagcactttg cagaaacgct gggcatggag gttcgtgccc
     4081 gtgccattcg cccgatcaac ggggaaaagg aatggatcgg cattctgacc gcgtatgata
     4141 cagaacagat taccatacag ccggaagaac aggaagcacc ggtgacactg ccgttggcag
     4201 acctatccta tgtgcggcgg tatgtaacag tagaatttta accaacggag gcataacgaa
     4261 gaaaatggat aacagttttt ttgacgcact ctccctgctg ggaagtgaaa acaacgtaga
     4321 cattgcacag ctggtggaaa aagtaaagtc tgccatgctg aaagcagcca gaaaggcata
     4381 tccggagtgt gaggacaaca tcaccgtcga aatcgatccg gcaaccaaga aatttgaaat
     4441 gtatgtgcgg cagactgtcg tagacgatga gccgatcgat gccaacgaag tcaatatcga
     4501 cgtggcacgc accgtagatc ccaacgccta tgtaggcggc accattgaca gacggctgga
     4561 catcgccaag ttcggcagag ccgctgcaca gtccgcaaag caatctatta agggtgacct
     4621 gcgggagatc aatcgggaac ggattctggg cgagttcgag tcgctggaaa acgactgcat
     4681 cacctgtgaa gtttctcggg tggaaaccaa cggcactgca acactgctgt accataagac
     4741 agaactgtat ctgttcccca atgagcagat cccgggcgaa acactgcacg aaggacagcg
     4801 tgtcaaggtc tacatcacca cgatcgccaa caagcagaaa aagccgatca tcaagatctc
     4861 ccgtgtccgc aaggaactgg tcaagcgtct gtttgaactg gagattccgg aaatttatga
     4921 cggcactgtg gagatcaagt ccatctcccg tgaagccggc tcccgcacca aggtggctgt
     4981 ctggtccaag gacgaaaacg tggatgctat cggtgcctgc atcggtccga agcgttcccg
     5041 tatttctgca attgtggctg aactgaacgg cgaaaagatc gacatcattc cctacagcga
     5101 aaagccggag gaattcattg ccaaggcact ggcaccggca acggttctgg acgtgcagat
     5161 cctgagcgaa gaagaacgca cctgcaccgt aaaggtaccg aacaaccagc tttcactggc
     5221 gatcggcaac aagggacaga atgccaagct ggctgccaag ctgactggct ataagatcga
     5281 catcaagccg gaattcgatg tgctgactaa gcacgagcac gatgcagcac cggcagaaca
     5341 gccggaagca ccgcaggcag aacagccggc agaagccgca gcagaggctc cggcggaaac
     5401 agcagaaaca gaagcaagtg caccggaggc gaacagcgtt gaagagtaaa aaagttccta
     5461 tgcggctttg tgtgggctgc ggcaccatgc agccgaaaaa agacctgatt cgggtgctga
     5521 aaaatgcaga aggggaaatc aagctggatt tcacaggaaa acaatccgga cgcggtgcat
     5581 atttatgtaa gaatatggac tgcttcgcag cagccaagaa ggggcaccgc attgagcgtt
     5641 ctttcggctg ccgtgtttcg gaagaagtct atgaggagat ggaacatgca ttacagacag
     5701 aactggcaag gaattctgag catctgtcgt aaagccggaa agctggcaat gggactggag
     5761 cccaccaaag atgccatgta ccgcgggaca gtgtccggcg tgctggtggc atcagacgca
     5821 tctgccaaga cgcggaaaga agcggcgttt tactgcgggc aggcacaggt gccctgtctg
     5881 gaactggcat ttaccaaagc ggactttgcc cagatgatcg gcagaggcag cggtgtgctg
     5941 gcgatctgtg acgacggctt tttccggaaa atgcagctgc tggcggaaca ggaaaaacag
     6001 gaaccacgcg gagaagcgta acagactttc atcatcacat gcggtgccgc agacagctgc
     6061 ggtccgcctg tgctgtggag atacattcgc aggagtggag tgccgcggcg gaggaacagc
     6121 ctacgccgga cattccttgg agaaaaggag gattttcgcc tatgacgaag aaagtaaaat
     6181 tgagcgacgt tgcacgggat ctgcagatcc cgacacagga actgatcgga ttttatcagg
     6241 aacgcgacga aaagggaaat acgaaaaagg gtgtgaccgg tctgacaccg gaggagatca
     6301 atctggcact ggagtattat tcccagaaaa cacaggtaga cagtctggac gcttactttg
     6361 cttccaagga taagccgaag tcgaagcagg aaaagaagtc cggtgacaag aagccggcgg
     6421 ataagaaacc ggcagacaag aaaaacagcg acaaaaagcc ggcagagaaa aaggcagcag
     6481 acaaaaagcc tgctgacaag aagcctcagc cggaaaagaa agcggaagaa aagaagcccg
     6541 cagctccgca ggctcagaag cacgaaaagc ccaagaagca gcaggcaaag gcacagaagc
     6601 acggcgagcg tctgcacgtt gaagtggaaa tggcatctgc ggacagcatt accaccgaaa
     6661 aacgccgcac ggtagacacc agaggctctt atgtcgatct ggataagtac aaccagcggt
     6721 atgagcagat cgctccggca agcaagtata ccaacgacag atattccacc aagaagcaga
     6781 agatcaacca gaagtctgca cagcggaata agcagcggta ttccagcaag cgggaaaccg
     6841 aacaggataa gctgcgtcgt ctggaactgg aacgtgccag aaagcagcag ctgcgtgtcc
     6901 gtattccgga cgtaatcact gtcggcgaac tggcaacccg tctgaaagtc accgctaccg
     6961 aagtcatcaa gaagctgatg atgctgggcg ttatggcgac cattaacgag gagatcgatt
     7021 tcgatactgc atctctggtg gcagaggaac tgggtgcaaa ggtagagcac gaagtgatcg
     7081 tgaccattga agaccgtctg atcgacgagg acgacgatgc cgaggatacc gaagaacgct
     7141 gcccggttgt ctgcgttatg ggtcacgttg accacggtaa gacttccatt ctggacagaa
     7201 tccgtaacgc tcacgttacc gcaggtgaag ccggcggcat tacccagcat atcggtgcat
     7261 atcaggtacg ctatgagggc aaggacatca cattcctgga tacaccgggt cacgaagcgt
     7321 tcacagcaat gcgtgcccgc ggtgccaata tcacagatat tgcaattctg gttgtggctg
     7381 ccgatgacgg tatcatgccg cagacagtcg aatctatcaa tcacgccaag gctgccggaa
     7441 tcaccgttat tgttgctatc aacaagatgg ataaggaagg tgcaaatccg gaccgcatca
     7501 aggaagaact gaccaagtac ggcatggtct gcgaagaatg gggcggcgac gtgatctgcg
     7561 taccggtttc tgcaaagacc ggcgagggca tcgatgacct gctggaaaac attctgctgg
     7621 tcgcagaaac cagcgaactg aaggcaaatc cggatcgccg tgcaaagggt actgtcgtag
     7681 aagcccgtct ggacaaggga cgcggaccga tcgctaccct gctggtacag aacggcaccc
     7741 tgcacgtcgg cgatgtcatt atcgcaggta ccgcagtcgg ccgtgtgcgt gtcatgacca
     7801 acgataaggg gcaggcagtg gaatctgctg gtccgtctgt accggtagag atcaccggtc
     7861 tggcagaagt gccggaagca ggcgatacgt tcaacgcagt agaagatgag cgtctggcaa
     7921 gagaactggt agaacagcgg aagcacgagg caaagcaggc gaagttcaac gagtaccaga
     7981 aggtcactct ggataacctg ttcagccaga tcgaacaggg cgaaatgaag gaactgtccc
     8041 tgatcgtcaa ggcagacgta cagggttctg tagaagctgt cacccagtcg ctggaaaagc
     8101 tgtccaacga cgaagtccgc gtccgcgtga ttcacggcgg tgtcggcggt atcaaagaaa
     8161 gcgacgttat gctggcaagt gcatcgaatg cgatcatcat cggattcaac gttcgtccgg
     8221 atcagactgc ggaagaaatt gcagcacggg ataaggtgga tattcgcacc taccgtgtca
     8281 tctacgatgc gatcgaagaa atcgaaactg cgatgaaggg catgctggct ccgaagttcc
     8341 gcgaagtggt aatgggacgt atcgaagtgc gtcaggtata caagatctcc aatgttggtg
     8401 ccgttgccgg tgcatatgtg ctcaacggca aggtgaccag acagtgcgag attcgcgtgg
     8461 tacgtgacgg cattgtcatt gcggaagata agatgtccag cctgaagcgg ttcaaggacg
     8521 atgtcaagga agtcggcgaa agctatgagt gcggtatcac actggaaaaa ttccgcgact
     8581 tcaaggaagg cgacatcttc gaagcattta tcatggaaga atacagagat tgacagaagg
     8641 gcaacgccgc ataaaattgg tgcggcattg ctgtgacatc atgttgctga aaagaaatgc
     8701 aacgatagaa aggagccgta cagcatggca aatttcaagg caaatcgtac cgaggaagac
     8761 atcctgcggg aaatgacagc aattttgcgg gaactgaagg acccccgtat cgatccgatg
     8821 ctgacggtcg tccgtgcaga actctcccgc gacctgtcgg actgcaagat cttcgtttcc
     8881 agtctgtccg gcaaggaaac cgccaaggaa tcctgcaaag gtctggacag tgcctccggc
     8941 tttatccggc gggaactgct tcgccggctg aagatccggc ggagtccggc actgcatttt
     9001 gtggcagacg attcgatcgc acatagtgca gagatcgacc gcatgctgca tcatatccag
     9061 ccgccggtgg aagaaccgga ggaaagcggc acgggagagg agtgaacggc gtgcaggagc
     9121 tgacattggc aaaaacagcc gcatggctac agcaatgtga agatgcttat attctgatcc
     9181 accgcagccc tgacggggac tgcatcggtg ccggttatgc actggcagaa attttacagc
     9241 agctgggaaa gcgtgcggca gtgctctgca acgatgaaat cccaccgcgg tatcagttca
     9301 tgctgccgga acagcccgca gcagcggact ttccgccgaa gtgcattatt gcggtagatg
     9361 tggcagatgc caagctgctg ggcagcagga tacaggaaca gtatggcagc cgtgtagatc
     9421 tctgcattga ccaccacgta tccaacgtgc cctacgccaa ggcaagactg ctggacggta
     9481 cggcttcggc ggtgtgtgaa atcctctacc gcctgtgccg cgtgttgccg gttcagataa
     9541 cggacagcat tgcagtgtgc ctgtataccg gcatggcaac ggataccggc tgttttcagt
     9601 atgataacgc cggtccggaa acccaccgtg cggtggcggc attgatggaa atatgccccg
     9661 atgtgcggta tgcgtggatc aaccggaaaa tgtttgcagt caagagcttt ggcagattgc
     9721 agctggataa actgctgatc gatcatctgg aatgttatct ggacggcaag tgtgtcctga
     9781 tctgtgtgtc acaggaattc ctgaaaaaat tccacgtgga cgaagcagaa ctggaaggca
     9841 ttgccggatt tccgctacag gtagagggtg cagagatcgg catcaccatg aaagaaaaag
     9901 aagccggaaa attccggatc tccatgcgtt cggcagatca ggtcaatgta tctgcgatct
     9961 gtcagcggct gggcggcggc ggccatgtaa aggctgccgg ctgtcaggta gagggtaccg
    10021 cagatgaggt acgccgcatt ttgctggacg cagtaaagga agaatggggg aaggcatgag
    10081 tcaggaaaca atgcaggggg ttctctgcat gcaaaaaccc gcggagttca catcgtttga
    10141 tgtcatcggc aagctgcgtg gcattctgca tatgcggcgg ctggggcata ccggaacgct
    10201 ggatcctatg gcaaccggtg tgctgccggt gctggtggga aaggcagccc gtgcctgtga
    10261 cattctgccc gatcaatcga aaatgtatcg tgctacggta cagttcggct gggaaaccga
    10321 caccgaagat gtctggggca cccgcacggc tgtctatccg gaaatgcacg tgacaaaaga
    10381 ggcactggac gcagtgctcc cgcagtttgt gggagcaatc cagcaggtgc cgcctatgta
    10441 ttccgcggtt tctgtggagg gaacacgtct gtacgaactg gcaagacggg gcgttgtgat
    10501 cgaacgtccg gcacggacag tacaggtgga ttccattgtg ctggaagcat gggacgaaga
    10561 caagcagacc gcggaactgt ctgttgcctg cggcaagggc acctatatcc gcacgctgct
    10621 ctgcgacatc ggaaaagcac tgggcggcgg tgccgtgatg acggcactgt gccggacaaa
    10681 ggcaagcggc tttacgctgg aacagtgcta tacatttgag caggtgacgg agttttgccg
    10741 gcggggaacg ctggcggaac atctcattcc cacagaccgt ctgtttacgg cactgccgga
    10801 gctgcacctg acagaacagc agtccaccct gtacggcaac ggtgtcaagc tggcattgag
    10861 ccgtgtggag ggcgttctgc cggagcagga tttgtaccgc gtttacggtg cagacggcag
    10921 cttcttcgga actgcacagg cagaccggaa tgcggacgaa ctgcgtgtgg gaaagaatct
    10981 gaaatagaaa ggcggaaacg tgcttgaaag gattccaacg catcactgca ccgctggcac
    11041 agaaaagtgc cgtggcactg ggcttttttg atggtgtgca tctgggacac cgtgccgtgc
    11101 tgggggcagc tgcccgctgt gcggcagaac aggaactgct ggcgtgtgca tttacttttg
    11161 cggcagattc cgttccggta aaacaaggcg tgccgctgtc ttatctctat acagataccc
    11221 agaaagcgga attgatgcag gactgcggga tccatggggt atactgtccg gcattttcgg
    11281 aactgtgcgg actggacggc gaggcattct gccggcaggt gctggtggaa ctgctccatg
    11341 cacaggaggt attctgcggc ggagatttcc gctttggtgc aaaagcagcg tggaatatcg
    11401 acagcctgcg ggaattcggc agccagttcg ggttcggcgt gcatcaggtc gcaccggtgc
    11461 tctgcggcgg agagaaaatt tcctccaccg cgatccggca ggcggtccgt gccggaaagc
    11521 cggaacaggc agcgaacctg ctgggcgtgc cctatcacgt cagcggcaga gttatccacg
    11581 gggcagcact ggggcgaacc aaggcagtcc cgacgatcaa cattcccttt gcagcaggac
    11641 agctggtgcc gcggtatggc gtgtatgttt cacggacgca caccccgcag ggcagcttcg
    11701 attccattac ggatattgga gtcaagccca ctgtcagcga agcacagctg ccggcggcag
    11761 aaacgtttct gctgcacttt tccggcgacc tgtacgaaca gccctgtcgg gtggaattgc
    11821 tgcatttcct gcgggacgag cagaaattcc ccgatgtgga cagcctgtac cggcagatcg
    11881 cacaggatca gaaacagtgt caggaatggc tgaaacaaca tgatctgtga gaacacagag
    11941 attatcatat gaaaggaagt actagcaatg tacaaaagag tgagaaccag atttgcaccc
    12001 agcccgacgg gctatatgca catcggcaac ctgcgtaccg cattgtttgc ctatctgatc
    12061 gccaagaaac aggacggcga ttttattctg cggatcgagg acaccgatca ggaacgttat
    12121 gtagaaggtg ccgttgatgt gatctatgat accctgcggg ttgccggtct gaactgggac
    12181 gaaggtccgg acatcggcgg tccggtggga ccgtatgtac agtcggagcg tatgggcatg
    12241 ttcaagtcct acgcggaaga actggtgaaa accggtcacg catattactg cttctgtgac
    12301 aaggagcgtc tggaagaagt gcggaaaatt caggaagctt ctcacattgc tcccatgtat
    12361 gaccgccact gccggaatct ctctccggaa gaaattcagg aaaagctgga cgcaggtgtt
    12421 ccctacgtca ttcgccagaa gatgccgttg gagggaacta ccacattcca cgacgacatt
    12481 tacggagatg tgacagtgga gaacagcaca ctggacgacc agatcctgat caagaccgac
    12541 ggcatgccga cctacaactt tgccaacgtt gtggacgacc acctcatggg catcacccat
    12601 gttgtacgcg gcaacgagta cctttcctct gctccgaagt ataatctgct gtatcaggca
    12661 ttcggctggg acgtaccgga atatgtacac tgtgcaccgg tcatgaaaga caagacacac
    12721 aagctgtcca agagaaacgg cgatgcttct ttccaagacc tgatgaaaaa gggctatctg
    12781 ccggaagcag ttatcaattt catcgcactg ctgggctggg caccctccgg cgaatataac
    12841 gaacaggaaa ttttcacgct ggacgaactg aaaaaagcat tcgagatcag cggcatcagc
    12901 aaggctccgg caattttcga cgatcagaaa ctccgtgcca ttaacggtgc ttatgtacac
    12961 aaaatggatc cggcagattt cgaaaaggca gcagagccgt atatccgcca gacagtaaag
    13021 cggacagaca ttgacctgtc cctgctgtgc agtgtgctcc agaaccgcac cgaagtattc
    13081 tctgatattc cggagcaggt ggactttatt gatgccatgc cggaatacga tctggaaatg
    13141 taccgccaca agaagatgaa gaccacaccg gaaaccgcac tggaagccct gaaggaagcc
    13201 ctgccggttc tggagagtgt cgaacagtgg gatactgagc acattcacga agcactgttt
    13261 gcaaagatcg cagatctggg cgtgaaaaac ggctggatgc tgtggccgct gcggacagct
    13321 gtttccggaa agcagtttac accgggcggc ggcgtagaac tgtgtgcaat tctgggcaag
    13381 gaagatacac tggcacgtgt tcgaaaggcg attgacgcac tttctgcata aggctttgca
    13441 atcaggcaat tggaaccttt cgtaagatta tcacttctct atcacaatag aatgataaac
    13501 tatacctagt ttttgtgtgc agggcaggag cgttctgtcc ggcacgcagc agaaggaggc
    13561 aatcacaagg atgattgaag tcaaacatct gacgaaacaa tacggcgaca agctggcagt
    13621 caacgacatc agctttaccg tagaggacgg cgagattctg ggacttctgg gaccgaacgg
    13681 tgccggaaag tctacgacca tgaatatgct gaccggctac atcagttcca cctccggaca
    13741 ggcactgatc aacggcattg acattttgga agacccgatc aaagccaagg cacagatcgg
    13801 ctatctgccg gaactgccgc cgctgtatct ggatatgacc gtcatgggtt atctgaactt
    13861 tgtctatgac ctgaaaaagt gcaagctgcc ccgccgttcc catctgaaag acgtgtgcaa
    13921 cctgtgccgc atcagcgacg tttccggtcg tgtcatcaag cacctgtcca agggctaccg
    13981 ccagcgtgtc ggtctggcac aggcactgat caacaacccg ccgatcctga ttctggacga
    14041 gcccactgtt ggtctggacc cgaagcagat cattgagatc cgcacactgg tcaagaagct
    14101 gggcaaaaag cacaccgtca ttctttcctc ccacattttg tcggaggtac aggaagtctg
    14161 tgaccgtgtg gtaattatca accacggaca gatcgccgca aacgacacca ttgacaacct
    14221 gtcgaaggcg gtttccggtg tcaaccgtct ggtggttcgc atcagcggtc cgaagaatga
    14281 agtgctacag gcgattcgtg ctatagacga cgtgacgaaa gtccgtgcag atatggaacg
    14341 ggagccgaag atcttcgaat tcgagatcga ggcaaaaccc gatgccgaca ttcgtccggc
    14401 tctgaaccag atggtcaagg aacacggctg ggacatttac aaaatggatc tgggcgtgat
    14461 gacgctggaa gatgcattcc tgaagatcac catgggcgag ggtgtcaagc tttcttcgga
    14521 aaagagcagt gctcccgccg cagaaaagtc cgcagctccg gcagctgaaa ctacggctcc
    14581 ggaagaaccc gcagcagcac cggcagaagc cgaaaaggca gaaacgccgg cagaagatgc
    14641 tgcacagaaa caagaaggag gcgaagcgta atgggtgcga tctatcgccg tgaagtcgga
    14701 gcgtttttca cctccagcat tgcctatgtt tttctggcag tgttctatct ggcagcaggc
    14761 tatttcttct tcggctcgtc gctggcaatg gcaacgaccg atatgtccaa cttgttcagc
    14821 agcctgtttt tcgtcatcac cattctgatt ccggtactga ccatgcgttt gtccagtgag
    14881 gaaaagaagc aaaagaccga acagggactt ctgacggcac cggtttccat cggcggcatc
    14941 gtcatgggaa aatatctggc agccctcacg ctgtatacca ttggtctggc gatcattttt
    15001 gtctatgctc taatcctgag ctttttcggg gcagtggcat ggggcatcgt gatctccaac
    15061 attctggcac tgttcctgct gggagcagcc tttattgccg tgacattgtt catctcgtcc
    15121 ctgaccgaaa atcaggtagt tgccgctgtg ctgggcatga ttgttctgct ggcactggct
    15181 ctgatcgacg tggtggcacg gtatgcagaa aatgtaccgg ttgttcccac cgttctgaat
    15241 gctctgtcgt tctacaccag atactatgat ttcactgccg gtctgtttga tccggctggc
    15301 gtgctgtttt atgtaagtac ggcagtgatc ttcaacttct ttacggtacg cgtgttcgaa
    15361 aaacggcgtt ggagttgaga ggaggacatc accaatggaa aaacagaaac atacagaaac
    15421 agcagaggct tctctggaaa aacagcagaa aaaactgaag aaccggaaaa agctgaaata
    15481 cggcggtctg gcaactgcga tcaccgtgat ctttgtggca gttgtggtac tggtcaacgt
    15541 cgtggtcacc cagatcggca agcggtatcc ggattttaag ctggatctga ccaccgcaaa
    15601 cctctatgcg atcagcgatg aaacactgga ctatatccgc aatatggata aagacgtgga
    15661 cattgccatt tcttccgatg aagctacttt cacttcggac aagaacaata agatgatcgc
    15721 ggaaaccctg tccaagtatc agggctattc tgaccgcatc agtgtgacct acttcgacac
    15781 caccaaggat ccggacgtat tgtccaagta tcaggaactg tacggcggtt cgatccagtc
    15841 caatgagatc atcatcacca gcggcagccg catcaaggtg tacaacacta ccactgatat
    15901 gtttgaagtg gatcagcaga agtaccagta ctatcagtac ggcatggcaa gctttgcaga
    15961 ctgcatcacc gcgttcaagg gcgaacagac cctgacttcc ggtatcatga acgttaccga
    16021 ttccaatccc aagaccgtgg gcatcattgc caccactaac ggcagcccga tctttgcaca
    16081 gaccaacact tcttctgacc cgaacaccta tgcgttctat gcaatggaaa acctgctgga
    16141 cgaaaacggt tatgatgtga gccgtctgga catgatcaac gacacactgg atacagaaaa
    16201 gtatgacatt ctggtgctgc cggctcctaa gacagacctg accaccgatt ctgtacagaa
    16261 gctgcaaaac ttcatgtaca atgacggcaa gctgggcaag cagctggtct acatcgcaga
    16321 ctacacccag tcctccacgc cgaatctgga cgcattcctg aaagagtgga atgtacaggt
    16381 ggattacagt tctgtcatcg acgaaaacaa cagcaccaat caggaagtca acattctcct
    16441 gtcccagagc aacaacagaa gcttcgtggc tccggtcgtg acagtgacag atgaagaaga
    16501 ctataccggt aatctggcaa atgccagcct gccgatcgtg gctcctatgg cacgtccgat
    16561 ccagacactg accgccaaca acggcagaac ggtaacggca ctgctgacct cgtcggatac
    16621 ctcttactgc tacccgctgt cgccgaagga ctacagcaca gacagcactg ctgatgtggc
    16681 tgacggttct gcggacagtc aggaagctac agaggcagcc accgaagcag ctgctaccac
    16741 cacttctttc gataagaatt ccgcacccaa gggacagaat accgttatgg cactttgccg
    16801 tgaccagcag tccaccggtg acgctttcat tgaaagtgat gtgatcgtgc tgggctccat
    16861 gtcgatgctg gacgtaaatc tggtacagaa ttcggcatac aacaatgcgg aatactttgt
    16921 aggtctgctg aactctgtct gcggcaagga agacagcatc gtagttgctt ccaaggactt
    16981 gacacagact tcgatctccg catctgcaac acagctgaaa gcgatccgtc tggtcgtggt
    17041 attcctcatt ccgctggcag ttgttgtggc tggcgtgatc gttgcagtcc gcagacggta
    17101 ccgttaaggg aggcacatca tgcgtaaaaa agcactggga atcatcggcg gcgtggtgct
    17161 ggtggcagca ctgggcactg catcgttttt cctgctgcgg aatgcaccgg aggaggattc
    17221 ttcttccgtg tcttcttctg acagcagcga ggaaagcatt tcgctgacct cggaagaccc
    17281 gaacgatgtg acttctattg acatcaccaa cggcagcggt tcttttgcag tggttcgcac
    17341 ggcagagggc accggcacag gagatgacca tgcggcatat gccgtgtccg gctgggaaga
    17401 tctgcctatg gatacctcca ccctgtggac actgtccaac aataccgctt ctatggaagc
    17461 caacggggtg gtagaagaaa actgctccga tctggaaaaa ttcggtctgg acgatgccga
    17521 ggcgatcgca gtcacactgc atttcgccga cggcagcgac tacagcttcc gcgtgggcaa
    17581 tgctgccgca gatgcttcca atacctactt tgcaccggca gacaaggaca ccgtctacac
    17641 ggtgaaaaca tcgctggtgg caaacttcag caagtccgca acggatttcc tgtccactac
    17701 gatgctggaa gcaccggcgg ataccaatga ttatcccatt gtcaacaagc tgactgtgga
    17761 acgcaaggat atagactata cgctggagct ggactatgac gaagatgccg caaacaacac
    17821 caatatgggc ggcactgtgg caagccatga aatggtatcg ccggtgccgg catatctgag
    17881 cgtagaccgt tcgacaccgg tggtgaccgg catgttcgga ctgaaagcgg aaaaggttgc
    17941 agttccgcac ccctcggcag aggacatcgc caatgccgga ctggacgacc cgttcgggac
    18001 ggcaaccatg gcgtgtgcag acggcaacac atatgtactg acattcggcg aacgctttac
    18061 cgaaaaagat gaggagaacg gtacggaaac ggcgtactat tatgcgatgc tgaacggtgt
    18121 ggacgcgatc tatcaggtga ccggggaaaa cttggtctgg gcgacaacaa cgcccacgga
    18181 tattgcttcc aagctggtgc tgggcaccta tgtctgggac gtaggctcac tggatgtcag
    18241 cgtcggcgag cagaagttcc agtttcaggt gaccggcagc gataaggaca ccgcagtcgt
    18301 taccctgaac ggagagagta ccgacaagga acgctaccgc cagttctact cgttcctgct
    18361 gaacaccacg gcggaaaccg tcaagctgga cggcgagccg gcaggggaga tgctgggaga
    18421 aatccacgtc aagacacagg acggcaaatt cgaacgggat ctacagttct acgccattga
    18481 cgagttcacc tgtctgatca ccgcagacgg caagtcagcg tacacctgcc gtaaaactta
    18541 tctggacaca ctgctgaaca acatgaacat ctacgacgac acaagtcagg actttacgac
    18601 gaactggagc taagtcaggg ggctgaccgt tccggagaaa ggaaagaaca gcaatgaatc
    18661 aggaattttt tatgtatcgg gggtatcctc tggtacgcaa gggaaacgaa atctattatg
    18721 ggtatatgtc agagccattc gtggtaatga tgcagatcgt gcatcagcag gaagtgaacg
    18781 ggctgaaggt tgcagataag attcgcgtct atcagatcgc aaccaaggag ccggatcctg
    18841 tgaaggcgat caccaagact tccgaccgac cgaatttgta tgaagccgta gatctggcaa
    18901 acgtctggct gaagcgtgct gaaaaggacg cagcagcaaa gtaaatccga tttgcctgga
    18961 aaagagcatc tctgcacggg agtgatcctg tgggagatgc tctttttgtt tggctgtatt
    19021 tttaaaaata ggctcccctg aaagggcacc cgcagggcgt gccttgtgct gagctggcaa
    19081 gccgtaggtt tgactgaggg atttttgcgt aaatctttta caccaagcaa aagattccac
    19141 aagaacccct ccaccgtctg acgacggttc ccctcgtact gagcggtccc ctctg
//
LOCUS       DAWBGR010000023        19105 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_980_length_19105_cov_5.583465, whole genome shotgun sequence.
ACCESSION   DAWBGR010000023 DAWBGR010000000
VERSION     DAWBGR010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19105)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19105)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19105
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_980_length_19105_cov_5.583465"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6240..6249
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10107..10116
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4379 a         5381 c         4542 g         4783 t
ORIGIN      
        1 aagcagatgg cgtgcaaagt cgccagacgc tctttgtgcg gtattgcctt tattgtgctg
       61 catctgcttc tgcgtccgga gccgtttctc cggagacatc ggctgcactg tctgctgcat
      121 gattatcggc agcaccagag gcctctgaag ctgccgcttc tccgctctgt atctctccgg
      181 tgtccaccgt cacaataaat ccgtcgctgt tgttgccgga aatggtgtac tttttatccg
      241 gcggcaccat gacataggtg gaatccgcag ctgtatcagc agccacataa tatacctgat
      301 agatctcgcc gtcccagttg gtaatggaca gcggcacgtc tgcactgtaa tttttcagca
      361 gtgtcagata ttcttccaga caaacgttgt tctcctgcat ataagcggca tggggctgtc
      421 ccacatagcg gtagtgccac ggctctgcct gaatgccggt agtgtcctgc ttgtcctcgg
      481 tgtagcggag cacatagcca tagtgggaac agttctcatt gatccacgca tattctcccg
      541 tgccgtcata gtcggactgc acgccgtcct catacaggga gaagtccagc gaatagccgc
      601 tttcgtgctc gctgtgtccc ggcagtgcca cacgggtgga agtctgttct ccggttgcct
      661 gcaagtcctc atcatacagt tcctgctgct gggcattggt acgatagccg ctgatgacga
      721 tcatatcctg atgaccagta gccacataga acgcgtccag catttgattc agggcatcta
      781 ccgcctgctg ccgcagactg gtttctgtgc cgctgacatg gtagttgtcc gtcttgtggt
      841 cgtaaatgct gacgatctct gacgggttct cctccacata ggcagtattc gcattgacca
      901 gaatcaaatc tcccgcatgc acatcttcat tggaaacaga aacattctca taaacattct
      961 gcggctctgt cgcagcttcg ctcatgtcgc ttgtcaattc catgccctct gttccctgcg
     1021 agatcggctg cagcgaatgg tacatatgaa atccctgata gccgacaaat gccaccagtg
     1081 ccacagagat cacgatttcc gccgccattt ttccgccggt acctctgggg cggcgtggat
     1141 tcggacgttt tgtcatctgc ttctgcggcg aagcagcctg ctttttgggt gcttttttct
     1201 tggctggttt gctttgtttt tgttcttccc tgctcatcga aaaacctcca aatatcaaga
     1261 cggcatcaca caaattccat atcgtgccgc cgagtgaaat ctcaaacatc ttttcagaaa
     1321 acacggaaaa aacttgtacg cagtatcgca tgcaaagttg ttactccgta tcgcctttat
     1381 attatagcat attttacgca aaatgcaaat actttttatg caaatttttt tcgaacggca
     1441 aaattgacat gctatggaaa cactggataa agcccgcctg acggctttat gcagtgtttc
     1501 cctacatcta taaaatcacc gccgcacgtc gccaatatcc gtgacaatgg catatcccgc
     1561 cgaagccacc cgccgttcca gacagcttct gcactgagcg gattcctccc ctgtgcaaat
     1621 tttgtcctca tacagcgtat acagctttcg cacggaaacc ggcgacagat tcggcatgac
     1681 cacgttggca ccggcacgca gtcccatttc ccgcccctgc gggtggatcg tccccaacgc
     1741 cgtagttgcc ggaatcaggg catagggaaa caacagccgc agaatgccga tcagccgcag
     1801 tgtctgtgca agactgccgt tgggctgttc ccgaaaaggc gtgtctttat gggtgagata
     1861 aggtccgatg ccgatcatgt ccggctccag ttcctgcaaa aagcgtaggt cggcacagag
     1921 attttccgtt gtctgatacg gtgagcccac cataaatccg gagcctacct gatagccgat
     1981 gtctttcagg tcaaacagac agcgtttccg gttttcagac gaaagcggct gggggtgcag
     2041 aaggcgatag tgtgccgcgt ctgcggtttc atgccgcagc agataccggt ttgcaccggc
     2101 acggaaaaac gcccgatagc tggatttttc ccactccccg acagaaagcg taatggcaca
     2161 gtccggaaac cgttcccgta tggcagaaac catttcacag acacgctgct ccgtgaaaaa
     2221 ggcatcttcg ccgccctgta ggacaaatgt ccggaacccc agtgcgtacc cctctgtgca
     2281 gcaggcaaga atttcctccg gtgtcagccg gtaccgctgc acatgggcat tgctccggcg
     2341 aatcccgcag taatagcagt cgttccggca atagttggtg aattcgatca gcccgcggac
     2401 aaatacatct gtcccgtacc gctgccgccg cacacggtct gccgcctgaa acagtgctgc
     2461 atcatagcgg tctgtttcca gcagcgaaca cagcagatcg tccggcaggt cccgctgctg
     2521 ctccagcgtt gcgatcagtt ctgacatgga aaaccccgtc ctttcctgcg gcagcaccgc
     2581 acaagctttg tacagcgttg ccctatgaga aaatattgct gccattcgaa aaaatccaca
     2641 aaaaagactt gcaattatcc cagaaatctt gtataataaa actatactgg ctgaataatc
     2701 acagtcaaaa aggagttata ttatgacaac agatgaaatt atgaaaaaaa ttcgggaagg
     2761 tctgaccggc gaatacagcg tagatatggt ctatctggaa tcacaggcgg acaagtaccg
     2821 ttccgcaaaa aatgcaaaag aaatcggaag tgcgatcgct gatctggcat atgagatcct
     2881 gccggacgac aagcgggaag tcctgaacaa aatgatgtac atcgacggca agcggctgga
     2941 cgttgtcttt gccgaagcca acaagctgat tcaggaaaag aaaatcgaag aatcgttcaa
     3001 gctgacagaa gcactgtaca ccaaaattcg catgaactat cgggaaaccg aatcggaagt
     3061 ataccttagc ttccgcaaca cgctggaaca tcagctgtat ctgtacttct accgcccgtc
     3121 caagaagctg gtgcgtccgg tctttgacct gtcgcagatg atcctgctcc acggctacaa
     3181 cctgctggaa ctgaaccgtg cagaagaagc tgcacgcgtc atcaacgatg cgatccgcta
     3241 caaccccatg aacacagatg cctatttcga actggcagag tgctataagt ccctgcggga
     3301 tcacgaccag ctgctttccg ttatcaagga aacgctggaa gttgccgtaa cgccggtaca
     3361 gatctcccgt tgttactgca atctgggctt ctactgtgtg gaagtcaagg actatgaccg
     3421 tgccgtttgc ttctattatg agagcctgat ctacgccgac caccctatgg ttccggcaga
     3481 actgcaccat atccacacca ttacccagaa aaaaatcgta ccgcccacac gggaagaagt
     3541 caacgaggct ttcgagcact acggtatgaa gtgcggtgcc aatgaggacg tagtcggtgt
     3601 tatcacagca ctggcaaagg aagccatcga aaacaaagac ctgccggcaa tccgtttcta
     3661 tctgcatatg cagtatggtc tgaccaatga tcctgagatc aaggaaatgc tggatcgcta
     3721 tgacaccctc gccaaggaac ggggacaggc ataatctttt cacacttact aagaaccgat
     3781 cacacggaca gcagcaatgc tgtccgtttt ttgtgcggtg ttgccttatt tttcatattg
     3841 ctgatatgcc tcatgcagcc gctttgccat ttcctgccgc agcgtttccg gagcaagcac
     3901 ttccatagct gccccgtatt tcatgatcca gcgatacagt ccgtggctga tgcccatttt
     3961 ggcacgcacc agaatatagt tttcggggtc ggtttcatct tcccgcacat gggcatagtt
     4021 tcccagctgt tcgatcatat cgtccagcag cacccgcttc acccgaagct gtacatcttc
     4081 gtacacctcc ggcacaaaaa tgtccgcgga aaagccttct gctttcgaca acttcggcat
     4141 gatgtcaacc cgttctcccg cggtgatgtc tttcatgcgg tcgatgcgat agttccgcac
     4201 attgccggtg tcctccacga tcactttcag ataaaaccgc tctgcggtgt agaccacctg
     4261 acatgggatc agatttcgct ttttggtata gtattcgatg acccgattgg tattatactt
     4321 gccgtacttg aaattgatct tccgcttttc ggcaatgatc tggtgcacac actccagatt
     4381 gtccagcaga tccagtgacg gcggagccac ctcactcaag gtgatccgcc ccaccagctt
     4441 ccgcacttcc gcttcggaac acagcccttc aaatttcttg aacagccgct gtttttgttc
     4501 gttgttcagg aatttgttga tagacaccga gtccaccaga aaccgaatct cattaaaggt
     4561 gaagccccgt tctttcatgt aataatacgc tgtgttgtgg gctcctttcc gaactccaac
     4621 ggtatacccc atggtgtcca gcctttcaat gtcccgcttg atactcagca ccgataccgc
     4681 tcccagattc gtctggttca gcaagtacgc ctgaatctct ttcagcgttg cccctttttc
     4741 cgcatcggta ttgtggctaa gataatccag cacatagaga attcgtgaca tggtatcctc
     4801 ctttacgctg ttaccggcag cgacggaaac agatgcacca gaaattgcag cagcaccact
     4861 acatctcgat tatccagcac gccgtctgca taacagtctt tgttctgctg ctgggcatcg
     4921 ttgacataca cctgccccat gatgattttt tgcagcaata ccgcatcgct catgtccacc
     4981 agaccgtcca gattgacatc accgacgata cgcggcaaaa ccggctctgt ctgtgtcgtc
     5041 gtcaccgttg tggcagcagc agtttccgaa gtggctgtac tgctgtcagg gtacctcgcc
     5101 ccctccagcg gttcctgtga ggaaatcagg agcatatccc agtacatata gtaatgattg
     5161 gtatcttccg gcaggtagta caccccaatg ccgatgtact gatacggttc tcccagcatg
     5221 gctttccggt gggtgggaga atcgatccac gcctgcacca cttcctgcgg tgtgtcgtat
     5281 cctgccgcca cattttccgc cgcataacaa ttggaatcca gcgtggaatc ctcaatgacg
     5341 ctgaaccatt cccgcccgtc cggtcggttg tgtccgtaag tctgcgacag ttcttccgca
     5401 cgctgtgccg ataaatctaa cagtaccggt gccagctgta acgccggcag tccgttttcc
     5461 tgccggaact ggttggtcag tgctgccgct tcctctgcaa aattggtcat gtctgccgct
     5521 gcggaaacct gccacttttg cggaaatccg ccgaatagta tagaaaacag taccgccaga
     5581 ccgcaaaaaa cggcaattcc cttttttcgc atctccacgc ctcctttgtg cgtttgtgat
     5641 attttagggc aataccgcac aaagagcgtc tggcgacttt gcacgaacaa tctttgtgcg
     5701 gtgttttctt tattatacta catgaggaca tttttttcaa tacatttcaa cgaacatttg
     5761 tgaaaatgta cagagaatgc cattcgtgtg atgcattgcc cttttcatca aggggaagcg
     5821 agctcttttc tttttttgct tctttttttc ttttcagtaa agaaaaaaag aagcgtaact
     5881 ccttgtgaaa tcgtttgatt gctgtagaga gtaacccctc agtcacctgc ggtgacagct
     5941 cagcacaagg cacgcccttc gggtgcccta aaggggagcc tagcaataca agcttgcaaa
     6001 cgccgtaaaa ataaaagttg accgactgta caagtaacac cttagaaaaa caacgctgca
     6061 agtacaagca agggtaacta tcggcgttgg caagcccaag gcggatagcg agtaaaacga
     6121 gcgaattccg ccgaatggtt tcgacctgat taccctagga tgtgttgaca ctaaacctga
     6181 cacgtgtctt ttggggtgat tttttcccaa aaaccaaaag cgtcatgcag tacaacaccn
     6241 nnnnnnnnnt ccttgcaata ttttttaact catcttagga attctttact gctgtttcgt
     6301 cttccttgcg aatctgagca cgcttgatct tgccgcccag cgttttcggc agttcgtcca
     6361 catactcaat aatacgcgga tacttgtacg gtgcggtggt gtgctttacg tggttctgga
     6421 gttccttggt cagctccgga ctgggggtat atccctttgt cagaacgaca gttgccttga
     6481 caacctgtcc gcgaatcgga tccggagccg cagtgatcgc acattccagc acagccggat
     6541 gttcgatcag ggcactttct acctcgaacg gtccgatacg atagccggag cacttgataa
     6601 catcgtcatt tctgcccacg aaccagtagt aaccgtcgga atcccgcatg aacacatcgt
     6661 gcgggcaata gctgccgccg cccagaattt cctgtgtcat ttccggattc ttatagtagc
     6721 ccacaaacag acctgtgggg taagcctttt tcaggttctg catcaccagt gcgccctgct
     6781 cgttgtcctt ggttcgtgtg ccgtcatcat tttgcagcac aatgtcatac agcggcgaag
     6841 gctttccggt agagccgggc ttcggcgtga tccacgggaa gtttgccagc agaaccgaac
     6901 cctcggtctg tccgaatccc tcgcgaattt ccttgccggt cagttcctgc caacgatagt
     6961 atacctccgg attcagcggt tcaccggcgg tggagaaatt ctgaatgctg gacagatcat
     7021 aagaagccac atcttcctgc aacataaacc ggtacatagt cggcggtgca cagaatgtgg
     7081 tcagcttgta gtcctgcatc ttttgcagca gatgtgtcgg cacgaacttg tccatatcat
     7141 aggcaaagat cactgccccg cagatccact gtccgtaaat tttaccccag ccgaactttg
     7201 cccagccgga atcggatacg ctcatgtgca gcttgttttc ctgtacctgc tgccaatatt
     7261 tcgcagtcac gatatgtccc agcgggtggg tgtaatcgtg ttctaccatt ttcggttcgc
     7321 ctgtagtgcc ggaggtgaag tataccagca tcaggtcatg tgccttggtg cctgcttcac
     7381 cggtgggacg tgcaaattct gtggggaact tttccacttc ctcgtggaag ttccgccagc
     7441 cctcgtgaga gccgcccact acgatcttgt gctgaatgct gggcacagag ggctgagcct
     7501 cgttcacctg ctccagtaca tacttgtcgt ccagacatac catggtgtgg atctgtgcac
     7561 tgtttccgcg gtagatcagg tctttctttg ccagctgcaa agaacccggg atcagaatgg
     7621 caccgatctt gtgcagagcc actgcacaaa tccagtactc ccaacggcgg cggagaatca
     7681 gcatgacaac agttcccttt ttgatgccaa ggctctggaa atagttggct gtctggttgg
     7741 acaggcggga aatgtccgtg aatgtgaaca ccttttcttc gccggcatca tcgcaccaga
     7801 ccaaggcttt cttttccggc tcctgctttg cccattcgtc tacaatgtca aaaccgaagt
     7861 tgaagtcgtc cggaatgttg acacgataat ttgctttgaa atcctcgtaa ctgtcaaaat
     7921 cgatacgagg caaatatttg tcgagcagca tatgcaaatg ctccttttct gttcagattt
     7981 ttctgcatgg aaaatcccat tgccgcacct gctgcggcag tttttcctga cagacactat
     8041 ttaaaaaaac agtgattatt tttattatac tacaattcac cgagccttgt caagattttt
     8101 tcccactttt ttgcacaact ttacaaaaga ggcagatctc cctgccgaga ggtctgcccc
     8161 atatccattt cttctctttt tctctgagga aacactgcaa gcagatgctt gcaaatttcc
     8221 cgtcatattt cacaaaaatg ctcgtgaata catccagtat tcactccgct ttttgtttca
     8281 tctgacgaaa aatttgacga cgcatctgtc gcacaatctt tgtgcggtat tgccttaatt
     8341 acttgaattt tgtccgcata gcacgggtgg aattcagaat cgcaatgaca gacaccccga
     8401 catctgcgaa aactgccgca ccgatatgtg ccagaccgac agcacccagt accagcacga
     8461 ttgccttgac acccagtgca aagacgatat tctcccgtgc gatgcccata gtctttttgg
     8521 cgatgtgcat cgccagcccc agcttcgaag gcttgtcgtc cagaatgacc agatccgctg
     8581 ccgcaattgc cgcatcagaa cccagaccac ccatggcaac gcctacgtca gactgtgcca
     8641 gcaccggagc atcgttcatg ccgtcaccca caaagaccag cgttctgccc tttgccagct
     8701 tggcacggta tgcctgcatg aactgtacct tatccgccgg cagacacttg gcgtgtacct
     8761 cgtccagctg caaagtgtcc ccgactgctt ccgccgccga ttcgctgtcg ccggtcagca
     8821 ttgccaccgt ggaaacccca gcctgccgga atgcctgcac tgcctgtgca gcgtccggtt
     8881 tgatcgtatc cgcaatcagg atatagccgg catatacacc gttcacagcc agatgcactg
     8941 ctgtaccaat ttccgtcggt gtttccggct gcaagccggc atcttccaga taagccgcat
     9001 tgcctgccag cacctgtttg ccgtccaccc tggcagaaac gccgtgtccg gcaatctcct
     9061 gcacgtcttc cacacgttcc gtatcaattg ccttgccgta tgcctctttc agcgactggg
     9121 caatggggtg attcgatgcc gattccgcaa gagatgccag ctccagctgt tcctccggtg
     9181 tgatttccac tgctttctgc ctggtaacgt ggaattttcc ctccgtcagc gtaccggtct
     9241 tatccatgac cactgtctgt gcgtgggaca gggcttccag atagttgctg cccttgacca
     9301 gcacaccgtt tttcgaagca caaccgattc cgccgaagaa actcagcggc acggaaatga
     9361 ccagtgcaca cggacaggaa ataatcagga acgtcagtgc ccgatagatc cagccggaga
     9421 acggctgacc ggtaatcagc ggcggcacta ctgccagaat gagggcagca atgaccacgc
     9481 agggggtgta ataccgtgcg aacttggtga taaattgttc tgttttcgcc ttgctggaag
     9541 cggattcttc taccaattcc ataattttgg aagctgtgga ctccgaagcc ggcttggtga
     9601 caaccagttc tactgcaccg ctcagattga cacagccact gcaaactgcg tcaccctctg
     9661 ccgcactgac cggcatagat tcgccggtga gagccgctgt atcaatggtg gtgtgtccct
     9721 caaaaacagc agcgtccagc gggatttttt cgccggggcg aaccagaata tgctcaccga
     9781 cttccacctc atctaccagt accgtttccg gcttgccgtc ccgcaggaca gtggcttcgt
     9841 ccggacagag atccaacaag tcggacactg cctgacggga acgtcccacg gcaatttttt
     9901 cgaacagttc gccgacctga tagaacagaa taacttctac agcttcggga tactgtccga
     9961 ttgcaaacgc cgcaatggag gcgatcgtca tcaggaaatt ctcatcaaat acctgcccgt
    10021 gtgcaatgtt ccgcacagcc ttttccagca cactgaaccc cggcaccaga tacactgcca
    10081 gaaaaatcag cagttccgcc caccacnnnn nnnnnncacg gcaaacagta ccgcaccaat
    10141 aatgatcttg atcagaattt tacgctgttg ttttgtcata tgaatcgcct cctgtttttt
    10201 atcatgggca ataccgtgca atgcttatgc agcgttgccc ttacttgatg ataatctcgc
    10261 agtccggttc taccttgctg atgcacttct gtgcagcctt cagcaccttg tcaaaatccg
    10321 catcgtctgc ctccagtgtc agcttctgtg tcatgaagct gatggttgcc gacttgacac
    10381 cgtccagctt ctggattgct gcctccattt ttgcggcaca gtttgcacag tccagatcat
    10441 tcagcttaaa tacctttttc atttgcgttt cctccatgca attcatttga acgattgttc
    10501 atatgttcat tatatacaat tgcaaggcaa ttgtcaaccc ttttcccgaa aaaaatcaga
    10561 ctccgccaca tcttcaaaga aacaaaagta tctctatcat ttcgcagaaa aaaagaacgt
    10621 atccaattcg aatacgttct ctgtctatcg ttattgcttt gctttattcg ctgtaggaga
    10681 aatcttctct ggtgagagcc gcttcggagt tataaaactc cattaaggtg ttattgtcgg
    10741 tgtactctcc gagataagga gcataccaca gcccgaagta cgaccacacc gcgttatccc
    10801 gaaacatggc attcatgtcc ggcacgttgc tgcactcgct gatcgccaga atctttccgg
    10861 cggacatgtt ccgcagtgcc acatactgtt cgtaacggct gccgtaagtc tgatcttttt
    10921 ccacgtacag atccagtgcc gcaatgtcgt acatgctgct gtccaccagg aagctgctgc
    10981 tctgtccgtt ccagacccag agcagattgt tcaggtggtg atactctgtc atacgggtgt
    11041 acagcagctt ccagagccac tggtaattct ctgccccgtc agagccccac cagtaccagc
    11101 cgccgccagc ctccggcagc ggtcgccaca gcaccggtac atcggcatct gccaacggct
    11161 gcaatgcctc tgaaatgctg tcgatgtccg acaccagagc atagcagttc tgcgagatct
    11221 tgccctctgt caccatggca gacaattcct ccagcgactt tccggcaatg tcctcgtctg
    11281 tcaccgccgt tttcaggtca taggaggtgt ctttctcgta gatcgttccc gttcccatgg
    11341 gagcgttcca gagccacgaa agccctacca caccgccgtc tttcgcccac gccagactgt
    11401 ccgccacagc agtggcgttc tgggaatccc cgccgttggg agaataggcg ttcatgtccg
    11461 caaaccggat cagcgggtat tttccggtga tgttatagat ctcgtccagt tccttgccgc
    11521 tgctgtccga ggcatactgt ccggtaatga ttttggaacc gtagttctgc tgcaaaaatt
    11581 gatacagctg ctgtgtcttg gcagttgcct tgctgttcac cggtgtggcg gaaatttcag
    11641 aagtcccgtc atataagctg gtattgttca ccagttcaat gcagtcaatg tccacaatgc
    11701 cgtccaccgg agccaccgac agttcacacg ctcctgcatt gaggaagatc ccctgaatct
    11761 tgacccgcat gaaattgttg gtggcatcgg ttttcatggt gtagacagtt tcgccgtcca
    11821 cctgaatctt acaggtcgaa gcggcacttg cccccactac aacggtgatg tcatagtgct
    11881 gtgtcgccgg aatctcggca ttcagcacca gactgctgtc attcttggca tccagcccac
    11941 tcaggtatcc gtttccggaa tagcccagtc ggggcatcat cgccacggaa cagccgctgg
    12001 gaacggcagc atcttctgcc tccagcttca tggacacgtc ctgtcgggtg atttccggca
    12061 cctgtaacgc cacagacggg tactgctgtg ccggaaccgt ttctgcttct gttgcaagct
    12121 gtgttgtggc agttgccacc gaagcggacg gctgggattc ttcctgttca atttttgcac
    12181 accctgtcag cagcacacct gccgccgcca gagcgaacac ttgttctttc ctcaaaattg
    12241 ctccatctcc tagagggatc gtccggagaa acacgacagg cagcacacag accacctgcc
    12301 atctgtttca cccgaatatt acgctttcag acgataagtc atgccctttt ctgtggggaa
    12361 ccgcagcagt gcaccgtcaa ttttggcatc aactgctccc gcatcactgg acacactggt
    12421 cactgtattg gtacgaaccg tgcacatatt tccgctgaga gagcggatca ccgcagtttc
    12481 cagcttgccg gcatgccata tcatatccac ttcaaagccg ccccgtgccc gcagaccgct
    12541 gatgctgccg tcctgccacg attccggcag tgccggcagc agattgatct cgccggaatg
    12601 actttgcagc aggcactcgg caattgccgc cgtgccgccg aagttgccgt cgatctggaa
    12661 cggcgggtga gaatccagca gattcgggtt agtagaccac gccagaagct gctggaagtt
    12721 ctcgtatgcc atgccgctgt ctaacagtct tgcccacaga ttgatgatcc atgcacgact
    12781 ccagccggta tgcccgccgc cgtggatcag ccggcggatc aaagtcgcac gggcagcctt
    12841 gcccagctcc ggtgtgtagt acagtgtgat cagatctgcc ggatacagag caaacaaatg
    12901 ggaaatgtgg cggtgtccga tctctacttc ctcgtaatcc tttgcccact cctgaatctg
    12961 cccgtatttg ccgattttca gcggcggcag tttttgcagc agggtttcca gttctgcggc
    13021 aaattctccg tcccgtccca gcagcttgct gctttcgatg acgttccgga acagcactgt
    13081 caaaatttcc gtatccataa acggtccgct gcacaggcag cccttggcac cgctttcggt
    13141 tttataggta ttttccggcg acacagacgg gcaggtcacc agttcgccct cgtcgttttc
    13201 cgtgagatag tccacgaaga acagggcagc ctcccgcatt gcctcatagt gctggtcgag
    13261 gaattccaag tcctgtgtga actggtagtg ctcgaagata tgcaggcaga gccatgccgc
    13321 acccataggc cagatcgtcg ccggcatcca cagatcctgc ggagccgtat cgccccagat
    13381 gtctgtgttg tggtggcaga caaagccgcg gcagccgtac attgcctgtg cggtttcccg
    13441 tccgtttgcc cgcatgcgtt cgatcaggtc gaacaacggc agatggcatt ccgacaaatt
    13501 gcagacttcc gccggccagt agttcatctg aatattgatg ttaatggtat agcggcagcc
    13561 ccacgccggc cacatgtcct gattccagat accctgcaag ttcataggct gtgttcccgg
    13621 gcggctggcg gacaacatca gataccgccc atagttgaag tacagcactg ccagcttgct
    13681 gtcatggatc tgtagcttgc actccttgtg gtcaccctcg tcgccgcgaa gccgcatcag
    13741 gcgttcatct gtaggcagtt cgctgccgcc ctcgctgttg tccggcaggg tgaaccgtac
    13801 ccgacggaac ttttcctgat actccgtcag atgccggtac agcagttcct catagctgca
    13861 gtctgccgca taggatgcgt ccagcttggc ggagtcctca tactgttctc cgtggtaaaa
    13921 gctggtgccg acgctgagga tcagcgttac ctcattggca tcttttacct ccagtgctcc
    13981 gcccaccgtg cggagtgtgc cgccctcggc aaatccggtc agagctgcgg cgaagaaaat
    14041 gccgtctttg ctgccggtac cgccgtcata gaccagcatg ttggggtagt ccgtgcaggg
    14101 acggcagtcg tcgtagtagt catctctgcc gtccaaggtt gcccgcaggt tgaccattgc
    14161 cggctcgctg gcggcaatgt gcatcaccag cacccgatcc ggttctgaaa cgaacacttc
    14221 ccgcacatag gtgatgtcgt tggcggtaaa gcgtaccgtt gccagtgcgt gttccagatc
    14281 cagactccgc tggtactgct ttgcctttcc ggcaaaatcc atgtgtaaat tcaggttgcc
    14341 caagggcatg tagtgtctgg aattcggcgt cacaccctgc attttttcga aagcgacttt
    14401 ttctgcttcc ggaatgcgtt cctctttcag cagcttccgg acttcttcca gtccctcccg
    14461 tgcatcagga ttgttccggt ttcgcttgcc gccggaccag atagagtcct cattcagctt
    14521 cagcacttca tctgctgcac cgccaaagat catggctccg attctgccgt tgccgacagg
    14581 cagtgcatgg tcaaaatcct ctgccggttt ttcataccag agcatcgttt ctgcgtccat
    14641 gtcaaatcct ccgttttttc ggggactccc gcccctgcat ttcctctgaa agctgcatca
    14701 gccgccccag catttttccg caccggatcc ggtcggcgtt ttcctgtgcc gcatttttgg
    14761 acggcgggat cacgcggacg gagtacggtg cttttgtatg ttttctggat tcattctgct
    14821 gcatcgccgt ttcctcctga aacagcatat gcggcacaat ggtgtatttt tcattgcaca
    14881 gctgcaaagt aacattgcac aaagcacaaa taagagccag agcccatttt gtatccggac
    14941 atgcaggcaa aatgcgtgca aaaaatacaa aacaggctct taaagcacgg tatcaatgct
    15001 gtgctgtttt tccatgacca gacctgtaag ccgagttctg tcgtgtgtga taatttatct
    15061 ggtctgtgag tcgcctcaca gctctagccg ccattacgcc gctgtccacc ggaccgatgt
    15121 taagacagcg tgtaccaata gacgttgcac cggataaggt ttacatagca gtacagtctc
    15181 ctgcactctg gtgtgctctt accacacctt tccaccctta ccgcaggaaa cccgcggcgg
    15241 tatctctctg ttgcactggc tctagggtcg cccccggctg ccgttagcag ctatcctgtc
    15301 ctatggtgct cggactttcc tcacagcgaa accgctgtgc tatcacacag tctgctcatg
    15361 gacttttatt ataccggatt tttgccgttt tgtcaagtcc ggatttttat accgttcaat
    15421 tctctaacga tctgttttat tttggcagtg catccttata atccggatat ttttctgcaa
    15481 ccagtttttc atatgccgca tacgcttccg tcagactatt ataacgttca cctttggtgt
    15541 atggtctgaa tccggcagaa ttataaatat gatctacgat cgcgacataa tttttaccct
    15601 tattcgatcg ttctgccgtt gtcgtttcag agatataaaa tccaccatct gccctgctga
    15661 tgatcacagg gatctttttc cccgcaccaa ctgcttcagt atttaccgaa gtccagaaca
    15721 gataccttcc ggaagcatca ttcggacttc ccaaatatgc ccatgtaccg taattcagca
    15781 gactgctttc tctgatctgc tcctgtactt ttggtaccat tgtagaattg ggacactgag
    15841 aatcgatctc aaagcgagca gatttatttg cgatcaaatc tgacaatata cttgtttgat
    15901 tcagggcact atgcagatct tcgttaaaat caatatcagt aactgtactg gtttcactct
    15961 ttcctttcca gtagaaaaca atgccggtcg aaggcgtata ggtgatctca cactctccgt
    16021 tcgccgcggg gatacctttc cagtgatctg tatccccttc ccaggtatag tgtttgatcc
    16081 ccgcaatcgt tacaggattg aacgactgtc agtcatctcg tttttgttca agctttaccg
    16141 ttttggacac acctgctgct ccatcgatca tcgatgccgt tctgactttg agatatgcct
    16201 cacgcacatt ggtgatgtca atggtttcat gacttcgttc aatatattgc agcagagcag
    16261 gcaccaaaat tgtagcaaga atccccataa tcacagaggc aatcaaaagt tcaacaagcg
    16321 taaatccatt tactttattc tgaactttac tcataacaac ctccacgctc ctttagcggc
    16381 atcactgcac aagggaaaca ctgcctaaaa tgttgtgcgg tgatgcctta tacctttttc
    16441 cactttcaac gatccacata catgacctgc acacaacggt atctgtgtat cgttttctca
    16501 ttatactata gtgcacataa aattgcaagc aaaaaaaatt caaaaagcac cgaaaaacgc
    16561 aggaaatgcg ttatttttta cagtattttt caaagacgtt cacaaatcaa aatgcttgca
    16621 tttttgtaaa atttgtggta tactaaaggc aacatctttt tctgaaaaaa ggaggtagca
    16681 cagttgaaac agatctattt tatctgcaat ctgcattccg gcagagcctc ggcgggggca
    16741 catctggcaa tgattatcga caaattcacc gctgccggat ttcaggtgac cgtacatccg
    16801 acacaggacc gcggcgatgc caccgcctgt gccaagctgg cgtgcgatgc cggctgttat
    16861 gatattctcg tctgctccgg cggtgacggc accctgaacg aagtcattca gggctgtatg
    16921 aaaagcaaga cccagtgtcc catcggttat attccccttg gctccaccaa cgacttcgcc
    16981 cgcggtctgg gaattcccaa ggatccggaa caagccgtag actgtatcat tcacggcgaa
    17041 gatctggtat gcgacgtggg cagcttcaac gacaaatact tcacctacgt ggcagcattc
    17101 ggagcattta cggagatctc ctacgaaacg ccgcagcatt tcaaaaacct gctgggacac
    17161 tccgcctacc tgctcaacgc gatctccaag ctgccgaaaa ttcgggcata ccgcatgcga
    17221 atcgaatacg acggcggcgt gatcgaggat gaattcctct acggcatggt gaccaattct
    17281 tcctccgttg cgaaatttct ggctctgtcc gatgttcgct gggacgacgg cttgtttgaa
    17341 gtcaccctga tccacaagcc ctctaatctg gtggaattcc acaaactggt catggcattc
    17401 tccaagatcc agctgggact ggcaaaacag tatctccact atttccgcac atcgcacctg
    17461 aaagtcactg cactggacga agagccggtt tcgtggacca ttgacggcga atacggcggc
    17521 acagaacggg tcaacgaggt gttcaaccgg aaacaggcgg tgacgttcca cgtggacccc
    17581 gcctgccgcg aacaggcaga agaaaacgcc gacagcggtt ttgccatagt caagcctctg
    17641 ccggacgatg tccagccatt cggcatagaa aagcaaaccg atgacgacgc ggaaaacagc
    17701 ttccaaccgg aagaacccga tccagattct tcagaatcat aaaaaatgat gcagatcgcg
    17761 tgcaaagccg tcaggcgagc tttgtgcgat ggtgccataa atcgctgcgg gtgtgccggg
    17821 tccctcaaag aactggggat tcggcggagc cggtaccatt tgattttctt catagagcat
    17881 gatcactgtc cgcaggatcg aaatgcccca gtaaatgctg aaaagtacac atacgacaat
    17941 gccgacgatt cccaaaatga taccgcagcg acgcatgccg cggctgtaat tccactgccg
    18001 ctttttcgcc cttgctgcat agaccagtgc cacgatgccc gtcacgacgg acagcaccgg
    18061 cagctggcaa aacaaaaacg acacgatccc aaaaatcaat gctctaagat catagtgata
    18121 ctccggtccc ataaacggag gtcggttgga atattgctgc atggcatttt tcctcctgcc
    18181 tttgatgaaa gcgaacacca catattcctg tgcggcgtta tccctgtatc agtatcttag
    18241 cagaattccg gaattttgtc aagaagtgtg tcgaattatg ggataggcaa ccgattcaga
    18301 aaggagttcc ctatggcaga acgtcagttt cacattcaat gcactgcgga ggacatcgga
    18361 cactatgtct ttctccccgg cgatccggac cgtgttccgt ggatcgcgtc ctatcttgaa
    18421 aacgcccaga aggtggcaca gagccgggag ttcaccacct ataccggcac gctggacggc
    18481 acccgcgtca gcgtcacctc caccggcatc ggcgggccct ctgccgcgat cgccatggaa
    18541 gaactggttg ctctgggggc acataccttt ctgcggatcg gcacctgcgg cggcatgcag
    18601 ccggaactgg tgccgggggc gttgatcatt cccacaggag cgatccgcat ggagggcact
    18661 tcgcaggaat acatgccgct ggcatttccg gcggtgccgg atttcggatt gacggaacac
    18721 ctggtctgtg cggcacgtgc tgctgcatat gattatcata ccggcgtggt gcagtgcaag
    18781 gattctttct acgggcagca ctcgccggaa accatgccgg tagctcccac cctgctccag
    18841 aactggcagg catggaaagc cgccggcacg ctggcatcgg aaatggaatc ggctgctctg
    18901 ttcacggtcg ccgccgcacg gcacgtccgc tgtgccacca ttttgcagat gctatggaac
    18961 caggaacgac aggcagtggt acgggacaag gaacacgccg caagcgacat gacacacgcc
    19021 attgaggtcg ccgtggcggc aatgcgtgct gtgattcggg aaaacaagga aactgctgta
    19081 taaaggaaac actgcataaa gcccg
//
LOCUS       DAWBGR010000024        19072 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_983_length_19072_cov_3.738707, whole genome shotgun sequence.
ACCESSION   DAWBGR010000024 DAWBGR010000000
VERSION     DAWBGR010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19072)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19072)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19072
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_983_length_19072_cov_3.738707"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4565..4574
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4251 a         5454 c         4649 g         4708 t
ORIGIN      
        1 aaataggaag tgtttatata cgaactggaa gtaatacatt tgaaattgat gatggtagca
       61 agccgatatt cgttgataag caaaagcgat aaatcctagc tttttcaaga aacaacaaag
      121 catgtactct gaaactcaaa tgctgtagca aggagcttaa agaggaaata ccagaatatt
      181 tctttgaaac cattcccatt attgagagaa tggactgtaa agtcattgac ttgaagaaat
      241 gcgaacaagc ttttcaaaag tggggaaaac aaacgcccac attggaagaa atccgcagtg
      301 aactgttttg atgaatgttc tcataccaga ataaaaaacg gctgccgtga ggaaatcccc
      361 tcggcagccc ttttctcttt tttatcatat cccacaacaa ttactgctgc ggaaatacaa
      421 ccttacgata agtatcaaag tgcatccact tgctgaagaa tgcctgatcc tgtccgccgg
      481 tttgagcata ctcgctcaaa aatgcctttg cttcctcggc ggagtgtacc agcacctcaa
      541 aaccgcgtcc gttgataacc ggcacgccgt cataggtata ctcacttgcc ggcaggatcg
      601 ccgtgatctt cttgtcacgg atccgcacag ccacgccgtc acggagtacg atgcagtcga
      661 agctgtccgg atagtggtag ctgtcgccgc tggtgggaat ctcgtcccct actgtatagg
      721 tctggctcac cggattgtac ttcaacacgt tcaggctctc ggtatcgtag tagaattcct
      781 cgaagatgtc ccctcttgcc ttgaggtcgc cgtactggaa ggagttgcgg tcggcagtgc
      841 tgctgacctt ctcgctgaca gcatcgacaa cgccgcatgc agaagtcata ttcgtcacac
      901 ggtcgatcag gatcagctcg cccagcacct tgtgattgga gaacagatct gccacaatgc
      961 cctctgtcag cagaatctcg caaacggcaa tgccgttctt ttccagtgtg tctgtttcct
     1021 gatgtgcacc ggtgttgaca tcaatggcat aatccacctt agtcagaatg ccggggatca
     1081 gcttggttcc cagcttcacc agatagtcct tgccgatgct cagcggctca tcatccatcc
     1141 acagcagaga aaccgtcagc ttcttatata ctgccacatc tgcgtccttg acaaaaacgc
     1201 agccgcggga aacgtccact tccttgtcca gcgaaatggt gaccggctgt ccctcaaagg
     1261 cttcgtccac agactggtct gtcatcagaa tgcccttgac ggtagcagtt tcattgctgg
     1321 gcagcgtggt gatcgaatcg cctacgcgaa ctgtaccggc ttcgatctgt ccctgaaaac
     1381 cacggaaagt gtgatccgga cggcatacac gctgtaccgg catatagaat cccttttcgt
     1441 ggctgtcgga atgaatgtcc acgttttcca gatattccag aagcggcaca ccatcatacc
     1501 acggaatgtt ctctgacttc ttggtcacat tgtcgccctc tgttgcagac agcggaatga
     1561 tgtatacgtt ttccagttcc agctccttgc ggagttctgc gatctgtgct tcgatctcgc
     1621 ggaaccgtgc ttcgctgtag cccaccaagt ccatcttgtt taccgcaaag acaaaatagc
     1681 ggatacccat cagcttgcag attcgggcat gccgtctggt ctgcaccagc acaccctgcg
     1741 aagcgtcaac cagaattaca gacaagtcag cgaacgatgc acctaccgcc atgtttcggg
     1801 tgtattcttc atgtccgggg gtgtctgcaa cgataaagct gcggttgtcg gtggtgaagt
     1861 accggtatgc cacgtcaatg gtgataccct gttcccgttc tgccatcaga ccgtccagca
     1921 gcagggagta gtcaatggca ccgctgcggc tgcccacctt gctgtccagt tccaatgtct
     1981 tttcctgatc tgcgtacagc agctttgcat catagagaat atgtccgatc agcgtggatt
     2041 ttccgtcgtc tacgctgccg catgtgataa atttcaataa acccttcatt agaagtatcc
     2101 ctcccgcttt ctgcgttcca tgctgccggc tgcctcgtta tcgataactc tggaggttcg
     2161 ctccgaagaa acggaactca gggtttcatc aatgatctcg tccagcgtgg tagctgttga
     2221 ttcaataccg ccggtcagcg gatagcagcc gagggtacgg aaccgaatgg atttatactc
     2281 cggcttttcg cccggcttca gcggcagacg gtcgtcatct accataatga tattgccgtc
     2341 ccgataaacg accggacgtt ctgctgcaaa atacagcggc acgatgtcga tgttttcccg
     2401 cttgatatac tgccagatgt ccttttccgt ccagttggag atcgggaata cccgcatgct
     2461 ttcgcccttt ttgatcttgg tgttgtacag cttccacatt tccggacgct ggttcttcgg
     2521 gtcccacgcc tgtgcttcgt tccggaacga gaagattcgt tctttcgcac gggacttttc
     2581 ctcgtcacgt ctgccgccgc cgaatgctgc ggtaaagccg tacttggtca gagcctgttt
     2641 cagagcctgt gttttcatga tgtcggtata agctgcaccg tggtcaaagg ggttgatgcc
     2701 ctgcttgaca ccttcttcgt tggtgtagac cagcatctca atgcccagct tcttggcagt
     2761 ttcatcacgg aaacgaatca tgtccttgaa cttccatgtg gtatcgatat gtaagaacgg
     2821 gaaaggcggc ttttccggat aaaatgcttt catcgccaga tgcagcatta cggaactatc
     2881 cttgccgatg gagtacagca ttaccggctt ttcgcattct gccgcaactt ctcggataat
     2941 gtagattgcc tctgcttcca gttcgtcaag atgtgacaat tcgtgcataa ggtttcctcc
     3001 tcaatattgg ttcgactgtt tggtatgaat acggatctca ctggtttttc cgtcggggtg
     3061 atcgatgatc cagcgtagga catcgccctc tttttccgtg tgtaccagac tgcccatgcc
     3121 gcccatatct gctcccagat aaaagcgaat ggcgtgtact gcacactctt tcagacagga
     3181 ggtgcagccc cagcagtctt tcgggtactt gataaacgct tttccgtctg cattcatgtg
     3241 gatcaggctg ccggggcaga ccatgctgca ttttccgcag ccgacacact gcttggcgtt
     3301 aatggcaatg ctcatagttc tgtccctcct ctaccagatc gcggaaaacg atttccagct
     3361 ttccgttttt catgcgggaa ttgacgtatt tcttccagtg tgcatcgtcc ttttccggat
     3421 agtccagatt ttcggcgaag ctgtgccagc gggtttcttt tctcgccttg aggtgtgcga
     3481 tcacgctgcg gcacaccaga agccgttccc gcagttcgta aatatacatc agttcctgat
     3541 aatccgctgc atgcagctgg tcggacagct ccgtcagccg tgcgatcttg tcatcggcaa
     3601 tgtccagttc cttttcgtgg aaccggtaat tggtgccgat accgccggca taagtgtcca
     3661 tgacggtctg cattgcatct tccagctgtt ccgtatcaaa cagcccgccg tctgttgtca
     3721 agaaatgtgc tgcttctgca aggtgctcct gcacctccgt ttcttccggc ggcaccggcg
     3781 tatgggaacg cagaaattcc agcatggatt ccgcggcaat ttcgccctcc gccaacgcac
     3841 cggtaacgta tttctgggga catccgccgg caacatctcc ggcggcgtac agcccctcta
     3901 tcgtggtggc acgcttggtg tccacccagt agccgctggc agtatgaccg cccacgatgt
     3961 acggctctgt gccctcgatc tccacattct gctgagaggg cttcctgccg ttctcgatcc
     4021 acttcagcgt ctgggacggt gccatgttca gataggcttt catcagggag tcgtcctgtt
     4081 ccggcgtgat gccctctgtc cgcagatagc agggacctct gccggcaagg ttctcgctga
     4141 ctgtgccgta gacacgttcc gaggtggtca gcccgtactt ggtttcatag acctcgccca
     4201 gcgaattcac ctgcttggct cccacgccct gtgccagtgt accggtagga gcaatggtgt
     4261 ccttgcaacg cagagcaatg aaccgcatct caaaggtggt catctctgca ccggcacgaa
     4321 tgcccatcgc gtagcctgcc cccgtattaa agggcggata ccacatcttg tggcgggaga
     4381 agccggggtg gttgggctta tacagaccag ccgcaccgcc ggtggcgatc ataacggcat
     4441 tggcttcaat gacatacata gtgccgtcct gaatgttgaa gccgaacgcc ccctggatcc
     4501 ggttgtcttt cacgaagaaa tccgtgatgt tgacgtgatt cttaatcgtc acctgcggca
     4561 tagcnnnnnn nnnngcctct acggcctttg ccaggatcgg cttcatgttt tcgccgttga
     4621 tcttcagatt gcggttgccg cgtgccacgt actttccgtt tgcatctttg aggatcacca
     4681 gccccagacg ttccatatgc tcggtcacct gattgagccg ttcagacatg gtgagaagca
     4741 gatcgccgcg aacgatcccg tcggcatctt ttcgggcata gtccacataa tcctgcggca
     4801 ctctgccctc tgtaatatac gcattcagtg cattgacacc ggctgccaga cagccgctgc
     4861 gtttgatatg agccttttca cagatcagca cttttgcatc agactgatct gcaatggtca
     4921 gtgccgcata gcagccggct gtgccgccgc cgatgatcag tacatctgtt ttgcagcgtt
     4981 cgattttcat ttcatcacca tctttatcct ctgttttcca acaggtattt ttccgcagaa
     5041 gccacacagt ttgcaccgtc cgccgccgct gtgattacct gccgcaatgc ctttgtccgc
     5101 acgtcccctg cggcaaacag tccctctaca gaggtcctgc cgtcctctcc tgcctgcaca
     5161 tagccgctgc tgtccagttc tgcaacgccc tccagcaggc tggtattcgg cacgctgccg
     5221 atcgccacaa acacaccgtc cagcgaaagt tcttccggac tgccgccctg cagcagcttc
     5281 agggtttcca cccgtttttc tccggtgatc tccgtgggaa cggcattggt caccagaaca
     5341 atattttcag ttttctgcac cgcttcctgc aatgccgcat tggcacggaa cgtatcccgc
     5401 cgatgcacca gatacacagt ctttgccatg cgggagagat acagtgcatc ttccagagcc
     5461 gtatctccgc cgccgatcac ggcaacaact ttgccctgat aaaatgcccc gtcacaggtg
     5521 gcacagtagg aaacgcctgc cagcctgccg ccggcaattt ccagccgccg gtaggaagtt
     5581 cctgcggcgt agatcacgct tctggcttgg agctctgtgt gatcctccag cgttgcggtg
     5641 aagtatccgt ctttcttttc cagatgtgtg acatcattct cacagaacaa cgcccccagt
     5701 tctgtggcat gttcccgaaa tttttcgccc agttcatagc cgccgatgtg gggcagtccg
     5761 gggtaattat ccacctgttc tgtcacggcg atctgtccgg cacccatggt atcccgttcg
     5821 agcaccagtg tctgtaatct ggcacggctg gcatagattg ctgccgtcag tcctgccgga
     5881 ccgctgccga tgataatgat gtcatacata gcttactcct tgatctgcaa gtcttccttt
     5941 tctgcatcgg tgccttcgat atggaacgaa ttcagcaccg gattctcccg atccatcagg
     6001 gaaagaatca tatagcttgc cttgctgtca tatgccagcc gcttatcttc ttccgacggt
     6061 ctggacggcg attcggggtg agaatgccag tttcccagcg gcaccagccc attggcacgc
     6121 atatccttga ctgctgccag ttgttccttg ggatccagac tgaaatgctc attggaatgg
     6181 tcaatgttcg tcagcaggta taccttcctg acgatcttgt ccgttccctg tacctcgccg
     6241 gcgatcagac cgcaggcttc gtccggaagc tctctggtcg catgtgccag cattttttca
     6301 aaatctgctt tgttcaattg aatcatagtc caacaccatc gcattctgcc tgttcatagt
     6361 cgatcagttc ggtaattgtg gggtgtgtcc cgcaaactgc acagccggaa gtttcagacg
     6421 gcagctttac cttgcggaat tccattttca gtgcatcata ggtcagcaga tagccagtca
     6481 gcaggttgcc gatacccaga atgtacttga tcgcctccat tgcctgtaag ctgccgatca
     6541 cgccgcccat tgcaccaatg acaccagcct gtttgcaggt cggtactgcg tccttcggag
     6601 gcgggttctt gaatacgcag cgatagcagg gaccttctcc cggcacatag gtcatcagct
     6661 gaccctgaaa ccggataata ccggcatgag agaacggctt cttcgccata acgcaggcat
     6721 cgttgatgag gaactttgcc ggaaagttat ctgtgccgtc aatgacgaaa tcatagtcct
     6781 tgataagatc catgatgttt tcgctggtga cgaaagtccg gtaggtgttg accttgacat
     6841 caggattgat tgcctccatg gtttccttgg cggactgtac ctttgccttg cccacatcag
     6901 cggtggcatg aatgatctgc cgctggagat tggacaagtc tacttcgtct gcatctgcaa
     6961 tgccgatcgt acctacgcct gcggcagcca gatacattgc agccggagca cccagaccgc
     7021 cggcaccgat gatcagcacc ttggcattca gcagcttctt ctgtcccttg acaccgactt
     7081 ctttcagaat gatgtggcgg gagtaacgct cgatctgttc gttggtaaat gccatgttac
     7141 tgtccacctc ccatgaaata gaggaactct acattgtcgc cgtccttgag aacggtctgc
     7201 tcaaatgtgc cgttttctac gaattcgtcg ttaatggtga cggtgacgta ctgcggggtt
     7261 tccacatttt cgtccacgat cagctgagcc acggtcagac cgtctgcgac ttccttctgt
     7321 acacctgcaa ctgtgatttt cataattcta tactccttta tgtgttattt cagggaatca
     7381 atgatctcct gcaattctgc ttttttgacc agacctctgc gtctgtcctg ctctgctccg
     7441 tttttgaaga agatcagagt cggtgccgcc tgcacttcgt actgttccgc cagcggcaga
     7501 tcaaagttga tgttgatctt tgccactttc agagcatcgc cgtatgcttc ttccagttcc
     7561 gccagaaccg gcgaaagccg cttgcaggga atgcagctgt cggaataaaa gtccgccagt
     7621 accagtccct ctgcttccag cacttctttc cggaacgtat cctgtgtgac acgtgcagcc
     7681 attcaatcac ccactttctt gacgatcagg tcgtatgtgc cgtcctcatt ctggagcagc
     7741 ttcaggatct tgtgtccttc ttccttaatg ctccgcggta cgttctgcac cggctcgccg
     7801 tcgttcatgt gaatggaaag gatctgtccg tcgtccagtt cttccagtgc cacctttgcc
     7861 ttgacaaatg tcaccgggca aaccacgtct gtaatatcta cggtatcatc aatttgaaaa
     7921 tcagccattg tatgcctccc agattttttc ttcaaatttt tctctgccta ctcgttccaa
     7981 tgtgaactgg aaccgttcgc ttgccttggc gttgttctca aagaactgaa tggtcgcatc
     8041 gcaaatcttc agaagctttt ccttgtccgc aataaacgga ataaccggtt cgcccttatg
     8101 aatgctgttg ccgaacagtc cgccaaagga aacgatgtag ccatgtacgg tatcccatgc
     8161 ttctgtggga catgccttga cgcagcgtcc gcagaagtta cacttgcttt cgtctacgac
     8221 gattttttcg ccctcgtgtg taatggcacc gctgcggcaa gccttgacgc agacaccaca
     8281 ctgaatgcag tcctcctcac gccatttcac ctgaatgccg cccttgatgc ccaagtcatt
     8341 ttcttcggac ttcaggcagt tgttctgaca gccggtgatg ccaaacttga acttatgggg
     8401 cagttcccgt gcaaagtacc gttcgtccag ttcctttgcc agtgtgtacg tatcgataca
     8461 gccgctggga cagattgcct cgccctgaca tgcggtaatg gtacggacac gcggtccgca
     8521 aacgcccggt tccacatcgc cctctgccag tgccgccttg acttcttcga tctgatccag
     8581 cttcacaaag ggaatttcca cgctctggcg ggaggtcaga tgcacatgtc cgtcaccgaa
     8641 ctgttctgcc acttctgcaa tttttgccag ctgctttgcc gtcagattac cgccgaccac
     8701 gcgaagccgg agggaaaagt gattcttctg tttctgccgc atgaagccgc ctttttttaa
     8761 ggtcgcataa tcaactgcca tttaacattc ctcctgcatt ttcgcgatcg cctgtccgaa
     8821 atcttcgatc aggtcttcaa tatcttcaat gcccacgctg atgcggatca aatcctcata
     8881 tacgccggca ctttgttttt gtgcttcatt gctgtgtgca taaatcgtac ttgcggggtg
     8941 gatcaccagt gtccgcacat caccgatatt ggtagcattg gtggcgaact gcaaatgatt
     9001 gatcaagcag aacgcctttt ccttgctgcc ggcacggagc gtcagaatcg caccgccctt
     9061 gccgccgaac tgtttctgca ccagcgaata atagggactc ttttccagtg ccggatagtt
     9121 gacctctacg ccgtccacgc tgtccagata ctgtgccagc tgcaacgcat tgtcacagag
     9181 ccgttccatc cgcagcccca gcgtttccag tcccacggag ttcaggaacg cattcatcgg
     9241 ggacaaacaa ccgcccacat ttctccagat cgcattccgc agctttgcca gataagcaaa
     9301 cttgccatac ttggcatagg gctcgaatcc gtcataccgg ctgctgtccc agtggaaatt
     9361 gccgctgtcc acgatcacgc cgctgatagc atttccgccg ccgttgatgt acttggaaga
     9421 agaatgtacc accacatctg ctccgtatgc aaacgggtgg atcagatacg gtgtagcagt
     9481 ggtgctgtcg atgatcagcg ggatcccgtg agcatgcacc agttccgaaa ccgctttcaa
     9541 gtctactacg tccagcttcg gatttccgat cagttccgta aagaccacct tggtgttctc
     9601 gttgatcagc ggttcgatct tctctaccgt gatcgattct gcaaagcgtg tggtaatgcc
     9661 aaacgctttc aggtcagaaa acagatcaat ggttccgccg aacagtcccg aaccggcgat
     9721 cacttcatct cccgcatgga gaatgttcag aagtgccatt gtcaccgccg tcatgccgga
     9781 agaacagctg actgctccga tgccgttttc cattgcggct acccgctttt caaaagcatc
     9841 tactgtggga ttgctgattc tcgtatatgc aaatcccggt gcctgattgt gaaaaacttt
     9901 ctctaatttt tctgccgttt catgtgcgaa tgcactcacc tgataaatcg gcatgatcgt
     9961 agaacctgta ctcttttcgc cgtcaaaatt ctgatgcagc aatgccgtat taaacttcat
    10021 gcgttcaccc acgctcctct cgttctcgga acattttttc gattgttccc ttgtcatgag
    10081 cctattatat caccttttca ctagtaagtc aataggtttt atatgatttg ttcgactttt
    10141 tcaatttgtt caaattcgac atttttaatc gatgtttaaa atagaaagta catgactttt
    10201 cgtgcaaaaa tcagacggtt caccagaaat cgcttctggt gccgtctgat tccggaatcc
    10261 gctttccctt agggcaacac tgtacaaagc tcgtgcggta ttgccttaga tgtagtacat
    10321 aaatgcctcg tcctgcggaa tagaattccg ataacgatcc atcatatcct gtaacgtaat
    10381 gctgctgcaa aactgccgca gataagcagt catctgatcc cagaggcatt cctgcacagt
    10441 tgctttcagg agagaggtgt ctgctgctcc gccggtatcc gcatcgccca gaatggtaat
    10501 atccagagcg tcaatgattt cctgaaacga aatcttgtct gccggacggg cgatcacata
    10561 gccgccctta aagcccttga taccacgaat caaatgtgtc tgccgcagtg caaccagaat
    10621 ctgttccaga tatttcacag aaatattctg ccgcttggaa atgctgctga ctgcaactgc
    10681 ttctccattt tccgaatgca gggcaatatc gatcagtgcg ataatgccgc attctacctt
    10741 cgttgaaatt tttatggtac ttcctcctct ctccggtttg cgtgatattg cagtgtttcc
    10801 tttagtctgc gaacagggtg gtggaataat accggtcgcc gctgtccggc agcagagcca
    10861 cgatcgtctt tccggcattt tccggcttct tcgccagttc cagtgcggcg tggagtgctg
    10921 caccggagga aatgcctacc agaatgccct catgcttcgc gatcagtttt gctgctgcaa
    10981 acgcatcatc gttctgtact tcgaaaatgt catcatagat tttggtgttc agggtctgag
    11041 gcacaaatcc ggcaccgata ccctgaatct tgtgcggacc gggctttccg ccggcgagca
    11101 ccggagaatc tgccggttct acggcaacga ttttcacatc cggattctgg gacttcagat
    11161 attcaccggt accgctgaca gtaccgccgg tgccgacacc ggcaacaaag aagtccacct
    11221 tgccgtccgt atcccgccag atctccggac cggtcgtctt gcggtgtgct tccggattgg
    11281 cagagttttc gaactgtccc ggaataaagc tgttgggaat ttctgccgcc agttccctgg
    11341 cacgttcaat ggcacccttc atgcccttta cgccctgtgt cagcacgatc tccgcaccgt
    11401 atgctttcag aatgttccgg cgttccacac tcatggtttc cggcatggta aggatcacac
    11461 gatacccctt tgatgcagca atggcagcca gtccgatacc agtattcccc gaagttggct
    11521 caataatcac agagccttct ttcagcttgc ctttctgctc tgcatcttcc agcattgcct
    11581 ttgcgatacg atccttgaca ctgcctgccg gattgagata ttccagcttc accagcacac
    11641 gggctttcag ttcctgtttc ttttccagat tggtgacttc caccagcggg gtgtttccaa
    11701 tcagttctac tgcaccttga taaatcttag acataacgcg taactccttt tccagaataa
    11761 tgatatagac aacaatataa aaaaaccaag ggcaatctgc cgaaaaatct gacaacgcac
    11821 cttacgcaca agctttgtgc ggtattgccc aagtttaaat gaatacggaa tcgtcctgta
    11881 aggactggtt ttccagcagg tagcattttt ctcctgcaat gacaaacagc cggtagagga
    11941 acacatctac agtttctcct accgtaatgg gttcttcctc caagtcccgc ttagcgatca
    12001 gcagatctcc gtggacacgc accgccaact cgatccagtc gccgcggaac gaagtccgtt
    12061 ctaccacgcc cttggaagcg gaactgccgt acttctgcac ctcgttcttg cgggtgatct
    12121 tcaggaattc cggacggaca atcgcctttt ccacaccgtc catacgctgg aatgtgtggt
    12181 acttctgata gtctgccagc acggaggtct gcccgaagaa cgatgctgta aacgcggtct
    12241 gcgggtgacg gtagatctca atgggcgttc ccatctgttc aatgtgtccg cggttggtga
    12301 tgatgatctc gtccgccacc tcaatggctt cgtcttggtc atgggttacg aaaatgctgg
    12361 tcacgcccag cttgctgatc atttccttca gccagctgcg gagttcctgc cgcaccttgg
    12421 catcgatcgc cgcaaatggt tcgtccagca gcagcagctg gggattcggt gccagagcac
    12481 gggcaaatgc cacacgctgc cgctggccgc cggaaagctg gctgggatac cgccgctcca
    12541 gtccccgaag tccgatcagg tctaccagtt ccatcacacg gctggcaatg gttttccggt
    12601 ctactttctg aatcttcaag ccaaaggcga tgttgtcata cacagtcatg taccggaaca
    12661 acgcgtagtt ctggaacaca aatccaatgc cacgcttgct cgccggaatg tcattgacca
    12721 cttttccgtc aatgcggatc tcaccgctgt cggggtgttc cagaccggcg atcatgcgga
    12781 gaatggtggt tttgccgctg ccgctgggcc ccagcagacc gatcagcttt cccttttcaa
    12841 taccgaagct gacatcatca gaagcctgat aattgccata tcgtttctga atatgattta
    12901 actctacgta catcagctat ccctctttcc tccgcggtgt tcgatcagac tgcgaatcac
    12961 cagaatcaca attgccataa gcaccagaat cgacgaagcc gcaaatgccg gtacatactg
    13021 aaattcattg aacaaaattt ccacgtgcag cggcagggtg ttggtcctgc cacgcaggtg
    13081 tccggacaat accgaaaccg caccgaattc gcccatggct ctggctgcac acagcaccac
    13141 gccgtacagc aacgcccact tgatatgggg aaacgtcacc ttgcggaaga tcgtccacgc
    13201 gttggcaccc atcagggcag ctgcttcttc ttcatctgtc ccctgcgatt ccagcaccgg
    13261 aatcagttcg cgggaaacaa aggggaatgt tacaaaaatc gttgccagca caatgcccgg
    13321 caccgcaaac accacatgga tccccagatc atgcagcagc ggataaatca cgctctgtct
    13381 gccgaatgtc agcaggaaga tcagaccggc aatgatcgga gaaatggtca ccggcacatc
    13441 gatcagcgta gtcagcactt tcttgccgcg gaactgatac ttggtgactg ccagtgctgc
    13501 aaacacgccg aataccgtgt tcacacctac ggcaatggca gtggcttcca gcgtcagcag
    13561 tacagctttt accgtgtatt tatcggtgac agctgcctga taggttttcc acccgtcccg
    13621 cagtgcctca gaaatgacag tcgccagcgg caggagcagc atgacgacca gaaacagcac
    13681 gctgacaccg atcagaaccc acttgacgat tttcgaacca acggtttctt catgcatact
    13741 cactgccctc ctttcagaat cttcgaactt ctggactgca ccagattggt caggaacagg
    13801 atcaggaacg ctgcgaccag cattaccaat gcaatggaag tggcactggt gtagtcatat
    13861 tcctgcaact tcgacatgat gatcaacggc gtgatctcag tttcataggg ctggttgccg
    13921 gcaatgaata ccacgctgcc gtactctcca agacatctgc cgaatgccag accgaatccg
    13981 gtcagggctg ccggcaggat ttccggaaat atcacctgcc agaaaatccg gatccggctg
    14041 gctcccagaa cccctgccgc ttcttcataa gtggaatcca gcttttccag aatcggctgc
    14101 accgtgcgga ctacaaaggg aatccccaca aataccagtg ccacggtaat gcctacccta
    14161 gtgtaggcga tctgaatgcc gaaccgtgca aaaaactttc ccaccagacc ggtgtctgct
    14221 gtcagggaag tcagtgcaat gcctgcaacc gctgtcggca gggcgaacgg caattcgatc
    14281 atgccgttca gcatccgctt catgggaaac cggtaccgta ccagcaccca cgccagaatc
    14341 acgcccatta ccgcattgat cgcagaagcg gcaaacgccg tcagcaggct cacccgaaag
    14401 cttgccagca cacggggcct ggtaatagtt tcccagaact ccgcggggct aagttttgcg
    14461 gtatagacca ccagcgatgc cagcgggatc aacacgacga tactaagaac ggaaagtgtc
    14521 actcccaagg ataatccaaa tccgggaatc acccgtttac tgcctttttt catagctgct
    14581 ccttattttt catagatctg atcgaaaatg ccgccgtcgg agaagtgcac cttctgtgcc
    14641 tcgtcccagc cgccgaaatc ctgaatgctg accagatcaa tgttcaagtc aaatacatct
    14701 gcatattctt tcaaaatttc cgaattggtc ggtcggtaat aatgctttgc cgcgattcgc
    14761 tgtgccgcat cggaatacag atactgcaaa tactcggtcg ccacatcgcg tgtaccgcgg
    14821 tcgtccacca cgtcatctac cactgccaca gagggctgtg ccagaatgct gatgctgggg
    14881 tttacgatct catagtcctc cggattgtcc cgcatggaga gatacgcttc attttcccat
    14941 gccaccagca catcgccctg tccgttttcc acaaaggtgg tcgtagctcc tctcgcaccg
    15001 gagtccagca ccagcacatt ctgatacatc ttcttgacga agtcctccgt ctgctgggtg
    15061 tcgccgttat atttccggtc ggcatatgcc catgcggcaa ggtaattcca ccttgcaccg
    15121 ccggaggttt tcggattcgg ggtaatgaca tctacgtcgt cccgcaccag atcgtcccag
    15181 tcctgaatgt gcttcggatt gtcctttcgc accaagaaca caatggtcga agtatacgga
    15241 gcactgtcgt ccggaaactc gtccacccag ccgtcctcga tcaatcccgc cttttccagg
    15301 gaatcaatgt cgtattccaa cgccagcgtg accacatcag cttccagccc gtttgccacc
    15361 tccagagcct gctttccgga accgccgtgg gactgaatga cttccacgtc ctgtcccgtc
    15421 tgctctttcc agtgctccgc gaatatcgta ttataatctg tataaagttc tctggtgggg
    15481 tcataggaaa cattgacgat gctcaccgtg ttatgatctc cgcagccggt aaatgtcccg
    15541 cctgccagaa ccgccgccag cagcagtccg atgcacttct gaatcttcat acagcatcct
    15601 tctttcctgc ggagaatcgg gcttttcccg cttctccgat aatacatacc attttgatat
    15661 gtttagtatg ttacatatca tactgcaaaa ctagtaattt gtcaatagga tttctccggt
    15721 tttgtagaat tgcacaagca aagaactgaa atcatagcga ttttataggc attatatata
    15781 aaaagctgtc cgatgccgtt ctatcccctc gcttgtctgc ataggataaa gcaaaggcag
    15841 caccgcagca gaatctgtac ggtgctgccc aagccaacgc cgccggacgg caaaaaagga
    15901 ggagttccat atggacctga aaatcaatcg ggaaatgctc cctgtgactg aaattcttct
    15961 ggacgaagta caggagcaaa gtgttgaact ggactacgta ctgccggact atgatccgga
    16021 catctttcgc atcatcggct gtgagatcca gccgaccatt gtcaattcca ctaccggaac
    16081 cgaccgcatc acctacgaac tgcgtgcaga cattcgcgtg ctgtactgcg gttcggagag
    16141 cacgctgctg caatgcgtgg cacagcagat gacattcacc agaaatgtag aactgccccg
    16201 ccctgctgag gacctgcaag tgcagatccg tccccgcacc acctatgcca actgccgtgc
    16261 cgtcagtccc cgccggctgg aagtccgcgg agccgtttcg gtttcgattc gagctgccgg
    16321 cgaccggaaa caggaggtca tcgaggacgt tttcggcatg cacgtacagc tgcgaaaagt
    16381 tcccgtggaa tacgccgcac agaaacgccg tgccaccaag catatcctgc tcaacgaaga
    16441 tgtggaactg ggacagtcca aaccgccgat ccgcagcatt gtccggagca gcgtccggct
    16501 gaaccacgag gagcagtcga tcctcgccgg caagctggtg gcaaagggcg aagcaacggt
    16561 gcagttcctc tacacatggc agcaggagga cggttccaac gggctggaac agatgcagtt
    16621 cactgtcccc tacagccaga tcgtggacat ggagcagatc gacgacacct accgcggcag
    16681 tgtggacaca caggtggtac ggtgcgacct gaagccttgt accggcaaaa acggcagcat
    16741 ggacagcttg cagtgcgaac tggaactgtg cctgcggtgc acagcgatca aaaccgcttc
    16801 cattcagctg gtgactgatg ccttttccac gctgtacccc tgtgagcagg aaacggtttc
    16861 tctccagatc gacatggcac cggtgccggt gcatgcggtg atgccgtgca gtgcctcgct
    16921 ccagcccacc gacaccgtgc cggaatgcat ctatgatctg cactgccacg tccgaaacat
    16981 caacatgcag ctgctctccg acaccaaccg cattcgcatc agcggcatgc tctgctgtaa
    17041 tctcctcgcc ggtgatgcag acggcatgcc catgatgctg gaaaaggagg aagcgttcga
    17101 ggtgtacacc gactcggaaa cgccggtaga caatgctgta ttgcaggcgg aggtgctccc
    17161 cacagactgc acctatcatc tggcttctga cggcaagatc accattcagg catcactgct
    17221 gctgcgggga tacctccgtc cctctgcccg ccacacctgt ctgtccggtc tgacggtgga
    17281 cggtgaaaac aaactggtgc gggacggcga ctatgccctg aagctgtact atggagtaga
    17341 gcacgaaaac gtctgggaca ttgccaagcg gtgccgcacc agcgtcgggg cgatcatgga
    17401 ggaaaacgac ctgacagacg aacagcttac cgctgcgggc atgctcttta ttcccattgt
    17461 acagtaacgg caggaggtac agaatatggc aaacacgatt tatcaggaca ttgcctgccg
    17521 cacaggcggt gacatttaca ttggcgtagt gggtcccgtg cgttccggaa agtccacgtt
    17581 catcaaacgg ttcatggaac agctggtcat tccccacatt tccaacgaag catggcggga
    17641 acgtgccacc gacgaactcc cccagtccgc cgccggcaga acgatcatga ccaccgaacc
    17701 gaagttcatt ccggaagatg cggtggaact gacgctggag gacaatgccc gcatgcgggt
    17761 acgcatgatc gactgcgtgg gatatatcat tcccagtgca ctaggctaca tcgaaaatga
    17821 acagccgcgt atggtacata ccccgtggtt tgaacaggaa gtccctttca acatggcggc
    17881 agaagtcggc acgcggaaag tcattgcaga gcactccacc atcgggctgg tagtcaccac
    17941 tgacggcagc atcaccgacc tgccccgtgc ggaatatgcc gagtgcgaac agcgtgtcat
    18001 tgcagagttg caggaactgg gaaagccctt tgccgtcctg ctgaactgtc tggatccgca
    18061 cactgccgcc gccaaggcaa tggcagaaca actctcccag cagtatcagg caccggtgct
    18121 gcctgtcagc tgtcccgatc tggatgaaaa cgacatctgt cagatcctga cagaactgct
    18181 gcgggtgttt ccggtgcggg agatcaacgt ctgcatgcca cgctggatct cctcgctgga
    18241 aaaaagccac tggctgcggg aatcggtctt ttcctgtatc cggcaggctg ccgcagaaat
    18301 ccacaccatt cgggatacgg ctcttttcgc cgaggcagtg agcacctgcc cctatgtcac
    18361 gcgggcagcc attcgggaga tcgacatggg caccggcagc gtctgtgccg aagtggaact
    18421 ggacgcatcg ctgttctacc gcattctggg agaaaccacc ggtctggaca ttcacggtga
    18481 aagcgacctg ctgcccattc tgctggagct gagccgaatc caaaaagaat acgccaaaat
    18541 cgaaccggct ctgcgggagg tagaagccac cggctatggc atcgtgatgc cggagcagga
    18601 ggaaatgaca ctggaagaac cggagatcat caagcagggc ggacgatacg gcgtgcggct
    18661 ccgtgcttcg gcaccgtcca ttcacatgat gcgtgccgac attcgcacaa ctgtcagccc
    18721 cattgtgggc acggaacgcc agagcgaaga actggtcatg tacctgctgg agggctttga
    18781 ggaggaccct acaaagatct ggtcgtccaa catctttggc aagtccctcc acgagctggt
    18841 gggcgaagga ctgcacaaca agctgtccaa aatgcccatg gacgcacggc tcaagctaca
    18901 ggaaacacta cagcgaatca tcaacgaggg ctgcagcggt ctaatctgct tcatcttgta
    18961 atcgtaaaac agcaaaacgc cggaacggtt ctgacacacg ttccggcgtt ttgcgttttg
    19021 tggaatgggt tatcgaaaag tcctctcgga ctgaaaatag ctatggcaat ac
//
LOCUS       DAWBGR010000025        19058 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_985_length_19058_cov_5.377467, whole genome shotgun sequence.
ACCESSION   DAWBGR010000025 DAWBGR010000000
VERSION     DAWBGR010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19058)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19058)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19058
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_985_length_19058_cov_5.377467"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4973 a         4832 c         5258 g         3995 t
ORIGIN      
        1 aaactccccc ggcggggcga acaggcggct gccgccgccg taacactacc cttttcacac
       61 ttgaaattgg ggcgtaaggt cagcggcgac tcgccgccgc ccgcccctct tgccaaggtt
      121 gtcgaaatat gctataatag aatcagaagt gattccgtca atggattcca aaagaaagag
      181 gtacaaaaag atgcgtaata ccacaagtta tgaaagcccg ttctgcaccc gctatgccag
      241 cgaggaaatg cagcacgttt tctccgctga caagaagttc tccacatggc gtcgcctttg
      301 ggtggctctg gctcgggcag aaatggaact cggtctgccc gtgacacagg agcaggtgga
      361 cgaactggaa gccaatatcg acaacattga ctatgacgtg gcagaagcag aagaaaaacg
      421 ccgccggcac gatgtcatgt cccacgtgta tgcatacggt caggtctgcc ccaaggctgc
      481 cggcatcatt catctgggtg caacttcctg ctatgtcggc gataatacag acattatcgt
      541 gatgcgtgac gctctggaga tcgttcgccg caagatgatt aacgtgctgg caaatctcgc
      601 tcacttcgcc atggaatata agggcatgcc ggctctggca tatacccacc tccagccggc
      661 acagctgact acagtgggca agcgtgctac actgtggatg aacgaactgc tcatggactt
      721 caatgaactg gaataccgta tcgcaaacct gaagctgctg ggctccaagg gtaccaccgg
      781 tacacaggct tctttcatgg aactgttcaa gggcgatacc gataaggtca aggaactgga
      841 agccaagatt gccaaggaaa tgggcttcaa cggcgttgtt ccggtttccg gtcagactta
      901 ttccagaaag atcgactcgc agattctggc aacccttagc ggcattgcac agtcctgcag
      961 caagttctcc aacgatatgc gtctgctcca gagcttcaag gaaatggaag aaccttttga
     1021 aaagactcag atcggttcct ctgcaatggc atacaagcgg aaccccatgc gttgcgagcg
     1081 tatcacttct ctggctcgct acgtgatgat cgacagcctg aatccggcat ttaccgcagg
     1141 tacccagtgg ttcgagcgga cactggacga ctctgccaat aagcgtgttt ccgtaccgga
     1201 ggcatttctg gcagtcgatg cgatcctgaa catcatgctg aatgttaccg acggtctggt
     1261 ggtatatccg aagatgatcc gtcagcggat catgcgggaa ctgccgttca tggcaaccga
     1321 aaatattatg atggacgctg tggaaaaggg cggcaaccgt caggaactcc acgaaaagat
     1381 ccgtcagtat tccatgatcg ccggcaagca cgtcaaggaa gaaggtctgg acaacgacct
     1441 gtgcgatatg ttgctggcag atccggcatt catgattacc aaggaagatc tggacaagat
     1501 cctgaagccg gaaaacttca ccggccgcag cgaacagcag accgaagaat tcgtggcaga
     1561 gtgcattcag cccattctgg acgcaaacaa ggatattctg ggcgaacact tcgagatgaa
     1621 gaactaagga aacactacat aaagcccgtc tgacggcttt attcttgcgt atcccataga
     1681 aaacaaccct cggcggaatc caatcgtcgg gggttgtttt ttcgtaggac gaaaaatcac
     1741 cgcaggtctt gacaacttcc tcaaaaaggt gtaccctaaa caaagaatca tcaagggagg
     1801 aatcatcatg ccgatccaga ttcgtgcggc agtgccggca gatgcaccgg cactgctcgc
     1861 catttatgcc ccttatgtgg agcaaactgc cattacattc gagtacgatg ttcccgcaga
     1921 ggcggaattc acccgccgta ttgtggacac cctgcaaaac tacccctatc tggtggcaga
     1981 acaggacggc gtgccggtag ggtacgccta cgccggaaaa ttccacgaac gtgctgccta
     2041 tgactggtct gtggaaacgt ctatctatgt ggaacagcac cggaagcgac agggtattgg
     2101 cagactgctc cacgatgcac tggaacagga attacggcga cggcatttcc tcaacatgaa
     2161 cgcctgcgtt gcctgtccca taggggaaaa tgacccctac ctcacacggg acagcatttg
     2221 tttccatgaa aaactggggt atcgcatggt gggcgaattc caccagtgcg gctataaatt
     2281 cggacggtgg tatcacatgg tgtggctgga aaagcatatc ggcagccacg agggaacgcc
     2341 gctgccggta ctgcggggat aatttcaaca atggatttgc gtgaaaggat ttgcgtgaaa
     2401 attttcctcg tcctattgac taatctgccg ttttcgggta tcataagaag taggaaattt
     2461 ttgcagtatc ccctgcacgg aatacggagg acacatacat gaacaaacaa ggacggttca
     2521 cagccgtttt gctgagcgga gttatcctct gcggcatcgg caccggctgt gccgcggacg
     2581 gctccaaaac tgccaaggca gactacacat ggagcaatgt tgccattggc ggcggcggat
     2641 atgtcaccgg catggagtat aaccccaagg aagaagggtt ggtctatgcc cgcacagaca
     2701 tcggcggact gtaccgccgg aaaaaggaca ctgactggga accgctgacg gactttctgg
     2761 gggcggacga atggggctat atcgggatcg agagtatggc gaccgatccg gtggagccga
     2821 accgcgttta ttttgccggc ggcacgtata tgagcgatga tgcggcactg tttgcctcgg
     2881 acgattacgg cgaaacatgg acacgctttg atgctccgtt cagctgcggc ggcaaccagt
     2941 ccggacgggg ctgcggcgaa cgcatgtgcg tcaaccccaa taacaacaag gaagtctggt
     3001 tcggcagccg tgacagcggt ctgtggaaaa ccaccgacta cggcaagaac tgggaagagg
     3061 tcacctcttt tccggaaaag ggcgactata aacaggagag caacagtatc ggcattctgt
     3121 gggtgacttt tgatccgact tccggcgaca tgtacgttgg cgtggcaatg acagacggca
     3181 cctgcattta taaaagcaca gacggcggcg acagctggac agcactggct gccaatgcgg
     3241 caggcatgta cccgctgcat gctgcattct ccaacagcgg aaagctgtat ctggcgtact
     3301 ctgacaattg cggtccgaat ctgtccccca cggctggggc agtctatgtg tacgataccc
     3361 agtcggacag cttcacggat attacgcctg atctgaacga cggcagacag ggcggcttcg
     3421 gcggcatttc cgtagatgcc cggaatccgg atacggtggt ggtttccacg ctgggctggt
     3481 ggtcggacaa cggcgacaat ctgtactaca ccaccgacgg cggtgacagc tggagcggac
     3541 tgttcaacct gaacaccaag gaaaaccact accagatgga cactgcacag gcggaatggc
     3601 tggactgggg cagaggtgaa aatcaggcga aaaccggctg gtgggtggca gatgtcaaca
     3661 tcaacccgtt caattctgac gaggtgatgt acggcaccgg tgcaacgatc tattccaccc
     3721 agaatatgac gaagatcggc acagagccgg tgaccattgc ctttgatgcc tacgggctgg
     3781 aagaaacggc agtgttcaaa atgctggcac cgcccagcaa ggacgattcg ccccagctgt
     3841 attccattat gggcgatctg accggctttg cccacaagga cgtgacacag tgccccgatg
     3901 atgcccactt catgaaaaac ggcaagccca cagatttaga tgtggcattc cagaacccca
     3961 acacggcggt atacctcagc gaggaaaaga ccaccacgat caatttcacg caggacggcg
     4021 gtgccacatg ggaaaccgta aagcacaagc ccgaccccgc cattggcgga caggtggcga
     4081 tgtccgcgga cggcagctcc acgatctggg tgcccaacag catttccgga aagccctatg
     4141 tgaccactga cctcggaaaa acgtggtact atgtggaagg actgggcttt aacgccaaaa
     4201 ttgccgccga tcgggtgaac ccgaaggtgt tctatgccac ctgtgacggg ctgttctacg
     4261 tgtccaggga cggcggcgtg actttcgaat ccacgggaca gatggtggca gattctgccg
     4321 aaccggtggc ggtgttcggg caggagggca acgtctggat ctccagcagc accatgatca
     4381 tgtacacgga ggacggcggc gaaagctttc agactgtaaa gaccctgccc accgtggaca
     4441 gcatcggctt cggcaagggc aaagaggaag acagctatcc ggtgatctat gccgagggcg
     4501 acgacggcga aaacggctac ggcatctatc gctcggagga caagggcaaa agctggatca
     4561 gaatcaacga cgagcagcat aagttcggaa acctgaaccc caagtacatc accggtgaca
     4621 gcaatgtcta cggcagagtg tacttctgca cagacggacg gggaatcgta atgggtgata
     4681 ttgcttcgtg aaaaaaagac attatggaaa atacattttt cgctggatca tgaccttgct
     4741 gctgctggcg gggctggtgt ttgccggcat cggcggcaca aggctgctgc acatctatct
     4801 gaccagcacc ggcaggcagg cggcagatgt tcccagcaaa caggattatc cggtgcgtgg
     4861 agtcgatctg tcctactatc agggcaatat cgactgggac gtgctggcat cacagggcgt
     4921 ggacttctgc tttatcaagg cgaccgaggg cattgaccac aacgacagcc agtttgcaca
     4981 gaactggcag acagcacagg cggcgaagat ctacgtgggg gcatatcact ttttccggtt
     5041 tgaggacacg ggaaaggagc aggcggaaaa ctttatccgc acagtgccgg tgacggaaaa
     5101 caccctgccg ccggtgatcg acgtggaatt gtatgagtcg ctgggcggca acatgccggg
     5161 caccggcacc gcaaggcaga atctacagga aatgctggac ctgctggaag cccactatgg
     5221 tgtaaagccc attctctatg ccgcccccaa cacctatcgg gcatatgtga aatcgtttgc
     5281 agcggactat ccgatctgga tcagcaacta ctattataag ccctatttcg actggacatt
     5341 ctggcagtat accgattccg gcgaactgga gggctatgac ggctatcaga cccatattga
     5401 cctgaacgtc tacgccggaa gcatggacga attccggcag cagttcggct tacaggcatt
     5461 ttagaaagga actattatga cagaaccaat ttttttgcag ggaatctgta aggactacct
     5521 ctggggcggc aatcgcctgc gggaggaatt cggcaaggaa agcgatgctg acaagatcgc
     5581 tgaaagctgg gaactcgcct gccacaagga cggcagcagc atactcactt ccggcagtga
     5641 tgcagggctg aacctgcggg aatatctgga caagaacggc accgcattcc tcggcaccaa
     5701 ctgtgcggac tgtaccactg tgccggtgct gatcaagctg atcgatgcca aacaggactt
     5761 gtccattcag gtgcaccccg atgacgacta cgccatgcgg gtggagggag aagccggcaa
     5821 gacggaaatg tggtacatcg tagatgcggc tccccacgct gccctgtact acggactgaa
     5881 acaggaagtg accaaggcgg aattcgaaca gcggatccac gaccagacct tgacagagat
     5941 tctcaatcgg gtggaggtca agccggggga actgtacttt atcccctccg gtacgctgca
     6001 cgccattggt gcggggatcc tgctggcaga gatccagcag aactccaaca ccacttaccg
     6061 tgtctacgac tacggcagac ggggagcaga cggcaagctg cgtccgctgc acattgaaaa
     6121 agctctggac gtgaccaatc ttcgtcccat tgcccccacg gtgcagtatc ctgcggaggc
     6181 ggtttccggc ggcacggtgc ggaagctgga agcgtgcaag tatttcgctt cagacgtggt
     6241 gactgtgcac ggcacggttg catttaccgt agggacagaa tcgttccacc acctgctgtt
     6301 cctgtcggga gaagccactc tgcaaacaga atccggcagc tatccggtga aaaagggcag
     6361 cagtgtgctg ctgcctgccg gactgggagc ctataccgtg accggcgaaa gcgtacagta
     6421 cgtggttacc cgcgtggacg aaaaattgag ttgacaattc cacatgatgg atttgcatat
     6481 tgaggaggaa acatatatga agtattacat tggtattgat ctgggcggca caaatattgt
     6541 tgccggcgta gtcaacgaaa gttatgagat tctggcaaag gcaagcgtca agaccaatct
     6601 gccccgtccg gaagaagaaa tcgctgcgga tatggcaaag gtttcgcagg aagctgcgga
     6661 aaaggctggc ctgaccatgg cggacatcga gtggatcggc gtaggcacac cgggcgttgc
     6721 caacagtgcc accggcgtga tcgaatattc caacaatctg ggcttcaata acactcccat
     6781 ggtgaagtat ctggaaactg cactgggcaa gcccaccttt attgaaaacg atgccaacgc
     6841 tgctgcatac ggcgaatatg ttgccggtgc tgcaaagggt gccaagaatg cagtttgcat
     6901 cacactgggt accggcgtag gtgccggcat cgtcattgac ggcaagatct acagcggctc
     6961 caacttcggc ggtgcagaag ttggtcacac tgtcatttcc gtagacggtc cgcagtgtac
     7021 ctgcggcaga aagggctgct tcgaggtata ctcctctgca accggtctgg ttcgcatgac
     7081 caaggaggca ctggcggcca atccggaaag tgccatgaag gacgaggaac gtatttccgc
     7141 acggactgcg ttcaactaca tgcgtgccgg cgatgctacc gcaaagcagg tggttgacga
     7201 ttacatcaag tatctggcag ctggtatcac caacaccatt aacattttcc agccggacat
     7261 tctgtgcatt ggcggcggcg tgtgcaacga gggcgatccg ctgctggttc ccatgaagga
     7321 gatcgtcaag aaggaagtct acacccgcaa ctccgcaaag aataccgaga tcgtgattgc
     7381 gaagctgggc aatgatgccg gcattatcgg tgcggcattc ctgggcaacg cacacgacta
     7441 atggcagcac tgcatcaagc cgcctgacgg ctttgcacga tttgtgtcac aacagcataa
     7501 aaatcaatct gataagcacc ccgagagcca aatgcaccgg ttttcggggt acttttgtat
     7561 caacattctg tacaattccc taaaatgaga tttgtgcatc ttgctggttt cttggaaaag
     7621 ggagggggtg aatggggaaa atctcttgac agcttgcaca aaaggtgata gaataaggtg
     7681 gataaggtga gcactgcttc agacggcaga ggactgttct gtgcaggtta cacaaaaaag
     7741 ataaggagtt cgatcacatg aagtttatga agaaactgct cagcaccatg acggctgcgg
     7801 caatcgggct gtccatgacc actgcactgt ctgtgggaac ctctgttgca tctgcggcag
     7861 acaagactgc ggtacagatg gtggaggaca tgggtctggg ctggaatctg ggcaacgctc
     7921 tggattcgac caacacatgg accagcaatc cctctccggc agatattgaa actgcctggg
     7981 gcaacgtggt tgcgaccgaa aacatgatca aggaagtcaa gaaggctggc ttcaacaccg
     8041 tccggatccc tgtcacctgg tgggatatga ccggtgcttc cggcactgca aagatggatt
     8101 cgtttgacgg tacagttgca gatcagtatc tggcaagagt caaggaagtc gtagactact
     8161 gcgtagataa cggtatgtat gcgatcatca acactcacca cgatgaggac tgggaaaagg
     8221 atacctccaa gatcgcaaca tttgaaaagc tgtggaagca gatcgcgacc tactttgaag
     8281 gctatgatga acatctggta ttcgagggca tgaacgaagt cagcttctct accagcgatg
     8341 ccatgaccta taatcaggcg tttgtagaca ccgtccgtgc aaccggcggc aacaacaaag
     8401 accgtctgct gatctgcacc gcagacagca acaacaccgc aaaggcactg tccagcagct
     8461 tctccatgcc gaaggacgac tccaatatgc tggcggtttc cgtacactac tatgaaccgc
     8521 cgacgttctg cgttgcagac accacttcca cctggggaca tcgggaaaca tggggcactg
     8581 ccgcagacct gagcaaggtg gaaaaggatt tcaatgacct caagaccaag tttactgaca
     8641 atggctatgg cgttgtgatc ggtgaatacg gtgcatgcaa tgccgacaag tacggcgtaa
     8701 acaccacacc gtataacaag gacgaagaat ccatcaaggc attctatcag gcaattgctt
     8761 ctacagcata caatatggac ggtatctgcc ccgttgcatg ggacgactct gactccggta
     8821 caatctgcct gttcagccgt aagaatatga aatggtttga cgaagacctg aagaacatct
     8881 tctccaacat tgcttccggc ggaaatgtag accccggcaa gcagaagacc gaccgtctga
     8941 cattccaggc aagcgacgtt gcagacggca gcgatctgct gatcgacctg aagccctaca
     9001 aggattacgg cgtaaacttg acttctgttg tcatcaacta tacaattaag ggcaacaagg
     9061 cttcttatgg taccggcggt gcagtacagt acaacattgt agacagcaat gacggttctt
     9121 cgcactgggc ataccagccg tattcgttca cagtcggaga aagtgtaacg acagtagaca
     9181 ttccggcaac caccaaggca actgacgagg acggaaacga attcgaaggt ccgctggata
     9241 tggactacct gaagatcggt cactggtatg actggaccga cccgaagggc ggcaaggtag
     9301 aatggtctta tgttgatgtc actctgatct ttgacaactt cttctatgta gacggcgatc
     9361 cggatcagac aacagcaaca acacagacta ctaccgaagc accgaagacc actactacag
     9421 aagctaccac aactgcacag ccgcagggcg acccgatcgg taaggcatac ttcattggta
     9481 tgatcggtgc agcaaacaac tgggcagagg gcgacgatga aggtgccgca gtgacaccga
     9541 tcactggcga cggcacatac agcgtagact gggacgttac cggcggcggt accgatacgg
     9601 ttcagttcct ggcagttttg atcacaccgg acggcgaaac cgaaaacttc accaccgata
     9661 ccttcaagga tctgaaggtt tctctggacg aagtctgggt tgacggcgta aagatcgaag
     9721 attacaccgt tagtgaaaag gcaatcaaca cccattatta tgaaggtgag ggtccgggcg
     9781 ttacccgtct gtacctccac gatgactggt ccaccaagat caaggatctt gcaagcaaga
     9841 ccaccattac aaaggatatt cgcgtagtat tcaccatttc cggcactggc cacgaaggtc
     9901 cggcagtgac taccacagaa cagactacca ccacaaccga aaaaattact accactacaa
     9961 ccaccacaac taccgaagca actactgaac cgactactac cacaaccact accacagcga
    10021 tcgaaacaac caccactacg acaaccacca ctgaagcaac tactgaaccg actaccacta
    10081 caaccactac cacaacgccg gagccgacta caacaacgac tgcggaaaca actactacca
    10141 ccaccgaagc aaccacaact acaacagaag caaccacaac cactccggga ccgactacca
    10201 ccacagcaga aaccacgact acaaccgtga cgatgctgac taccacagaa gccactacaa
    10261 cgacaactac caccgaagaa actactacca ccacaaccac cacaactacc actacaacaa
    10321 caacaaccac tactactacc acaaccacga ccacccagta tgatggcccg atgccgaaaa
    10381 cgctgttcgg tgatgtgaac atggacggca gaatcgacat cacagatgct gttctgctga
    10441 acaagatggc agctggtgca gttgaagcaa accagtccca gcgtctgaac ggcgatgtca
    10501 acggcaacgg cgagatcgat tccgctgacg gtctggtatt gtttatgttc gtgatccagc
    10561 agatcaacac cctgccgtat caggcagaaa tctgatcggt gaagtttatc cgatgatttt
    10621 ctcatcataa aaagagcact gtgtcctgca tactgtgggt acagtgcttt ttcttttgtg
    10681 aaaatacagt ctgcacgggc gttttacatg agtatgttca agaatggcat ttttcacaat
    10741 gtaaaatttg tgaaaaatgc cggagaagag gaaaaccaac gcaatatcta acaaaaaagc
    10801 gggattttac ttaaaaaaca gcccccaaat ccggcggata cttatcatta ttccggaaag
    10861 tgttgtgcct ttgtgaaatg tgcaacaaaa caagaaaaga aactagtgga aatcgtcatg
    10921 aaagtcgatt gacttttttc gaaaatcgtc ctataataaa ggaaacagca ataaacacgc
    10981 tgttgatttt cgtttttcag gagaattgga aaaggcaggg aatcctgcgg aattccgcgg
    11041 aaaaccactg tttctctctc cggaaaaacg atgcagaaaa ggtggtggat acaatgggtg
    11101 aagaactgca ggaacagctg caaacccgga tcctctggga gagtccgttt gacgtaccgc
    11161 tggtgggacc gatccacatc agtgaatcgg tcgtcatgac atggttcctt atggcgatca
    11221 ttatggtgct ggtactaata ctgacacgga atctcaagat cgtaccggga aagcggcagg
    11281 cgtttctgga aatggcggtc acatggctca caaacttctt tgagggcaac atgggcaaag
    11341 ccggcagacg ctatgtgccc tatctgagta ccgtgctgat ctatctggga atcgccaata
    11401 tgatcggcat gttcgggttc ggcatgaagc ctcccacaaa ggacatcaac gtcaccgtgg
    11461 cactggcact gatgagcatt atcctcattg aagcgtcaac atttcgggct cagggcggca
    11521 tcaagggctt tttgaagtcc ttcacaaagc cgatggctct gctgacaccg ctgaatgtga
    11581 tggagatcgc cattcgtccg ctgtcgctgt gtatgcgact gttcggtaac gtactgggtg
    11641 ctttcgttgt catgcagctg attgaacatg tctgtggtct gattatcccg atggtgttca
    11701 gcatgtactt cgatgtgttc gacggactga ttcaggcgta tgtctttgta ttcctgacat
    11761 cgctgttcat ggcagaaggc atggaagtcg aggaggagag tgtgccgaag ttacagcagg
    11821 catagcttgc tgcaatccca ctcggcagct acagaaagga gcgttcccta tggtaggagc
    11881 aggtatcgca gtattgacag gtatcggtgc aggacttggc atcggtatcg caacgtccaa
    11941 ggcaacagaa gccattgcca gacagccgga ggctgccgga gcaatcaaca aggcactgct
    12001 gctgggctgt gcactggcag aagcaaccgc catctatggt ttcgttgttg cactgctgat
    12061 ggttatcatg aagtaaaaaa cacgcccccg caaaacaacg cgggcagaaa ggtaggaatt
    12121 gaaaatgata ggagcaggta ttgcagtatt gactggtatt ggtgccggtc tgggtattgg
    12181 tatcgcaaca tccaaggcaa cagaagccat tgccagacag ccggaggctg ccggagcgat
    12241 caacaaggca ctgctgctgg gctgtgcact ggcagaagca accgctattt atggtttcgt
    12301 tgttgcactg ctgatggtca ttatgaagta aaaggaccac cttctgaatc tgagagaaag
    12361 gagcacggct tttcttggaa aaagctgtga gagtggatat gttagatttt gatttttgga
    12421 ccattttgtt ttccgtcatc aatatcctcg tgctgttttt ctttctgaaa aaattccttt
    12481 tcggcagagt aaatgccatg ctggaaaagc gggcacagat ggtgcaggcg gatatggacg
    12541 aagccaagca gcatgccgct gatgcggaaa agctgaagaa tgcatatgag gaaaccctga
    12601 gtggtgccaa gcaggaagca aaacagatca ttgcgaccgc agagcagaat gcacacgcac
    12661 agggcagcga gatcactgcc aaggctcaac agcaggcaga cacgctcctc aaagaagcac
    12721 agaaggaaat cgaacgggaa cgccggcaga cactggacgg cgtacagggc gagatcgccg
    12781 atctggcact ggctgctgct tccaagctga tggaacagaa agtggacgac cagaccaacc
    12841 gcgagctggt gaatgcgttc ttgtctgagg aaggagcagg agaatgaatg gtgcagtgac
    12901 atcttatgca accgtgctgc tgacgctgcc aaatgcgtca gatgccgtgg cagatgcaca
    12961 gcaaaccttt cagaactgcc ccctgttgac ggattcccta agcaacccga cgatttcgga
    13021 ggcggaaaag tttgcagtga tcgaccggat ctttccggaa tccctgcgga ccttcctgaa
    13081 ggttgtctgc caaaacggtc agatcgacag catactggag attttttcag aatatcaagc
    13141 attggaacgc cggcaaagac attgtgctca tgccgtgctg gaatatgtaa cgccgctgac
    13201 agacgcacag ctgacagcga tgaaaaaaat gatctgtgca aaaaccggcg aaccggaggt
    13261 acagttggaa ctgcaccaga atccggaact gctgggaggc tttatcctgc atatcggcga
    13321 tgacgagtat gaccgcagcg tgcgttctgc cgtccgcaat atgcgaaaga gcctgatacg
    13381 gaggtgaggg taagacatga gtaccatgaa tgcaagtgag attatatccg ttttaaaaag
    13441 cgagatagag gacttcgacc ggaagtccgg cgtgcaggaa accggtacag tcgttcgtgt
    13501 gggcgacggt atcgcaacgg tattcggcat gcagcatgcc atgtacggcg aactggtaga
    13561 atttgaaacc ggtgtacgag gcatcgtaca gaacttggaa accaagacca tcggtgtcgt
    13621 tctgttgggc agtgactatg gcattacaga gggtacttct gttgtccgta ccggaaagcg
    13681 tgccggtatc ggcgtaacgc agaaaatgat cggcagagtt gtcggtgcac tgggaaaccc
    13741 gatcgacggc ggcgatcccc tgacagcaga ggattatttc ccgatcgaac acgaagcacc
    13801 cggcgtggcg taccggaaat cggtcaatgt accgttacag accggtatcc tttccattga
    13861 ctcgatgttc cccatcggac gcggtcagcg tgaattgatc atcggcgacc gtcagaccgg
    13921 taagacatcg attgctgtgg atacgatcat taaccaaaaa ggacagaatg tcatttgtat
    13981 ttatgtcgca atcggacaga agtcctcgac agttgcaaag atcgtttcta ccctgaaaaa
    14041 gaatgatgca atggagtata ccattgtcat gtctgcatct gccagcgaac cggctccggt
    14101 acagtatatc gcaccctatg ccggcacggc gatcgcagag tatttcatga gtcagggcaa
    14161 ggacgtgctc attgtatacg atgacctttc caagcacgcg gtggcatacc gtgcaatgtc
    14221 cctgctgctc caccgtccgc ccgggcgtga agcttatccg ggtgacgtat tctatctgca
    14281 ctcccgtctg ctggaacgtt cctgccgtct ggacgatgca cacggcggcg gttcgatcac
    14341 cgcactgccg atcatcgaaa cacaggcagg cgacatctcc gcctatatcc cgaccaacgt
    14401 catctccatt acagacggac agatcttcct cgagagcgaa ctgttcaacg ccggtatgcg
    14461 tccggcagtc aacgtcggtc tgtctgtatc ccgtgtcggc ggtgccgcac agaccaaggc
    14521 gatgaagaaa gcctccggtc agatgcgtgt ggatctggca cagttccgcg aaatggaagt
    14581 ctttacacag ttcagttccg atctggatcc tgctacgcag gcaatgctgg atcacggtca
    14641 tgtgctgatg gaactgctca agcagccgct gtatcaccct ctggcaatgt ggaaagaggt
    14701 cgtgatcctg tccgcagcct ccaacggtct gctggacgat gtggcattga gcgatctgcg
    14761 ttcgttccgc gagggactga tgaactatgt ggaggcaaac ggtgcggcga tcgttaccga
    14821 aatgaaagaa accggcaagc tttccgacga atccagagaa aagctgctgg aactggtgcg
    14881 tacctataag aagcaggtga actaaaatgg gcaacatgcg tgagatccgt gaccggatca
    14941 agagcatcaa cgacattatg aaaatcacca atgccatgta tctgatctcc tcctccaagc
    15001 tgaaaaaggc gaaaaaggat ctggcggcaa cacagcccta tttcaataag ctgctgtatg
    15061 ctatgcggag tatcctttcc cgtgcaccgg aggacatcga tatgcggtat ttcgatcaac
    15121 gggaggagat caagcccgaa gaccggaagg tgggctatat tgtcatcact gcggacaaag
    15181 gcatgtgcgg ctcctataac cacaatatca tcaagctgac agagcagaaa atggcggcac
    15241 acaagcaaag tcacctgtac gtcatgggac aggtgggaag aatgtacttc cagcggcgtg
    15301 cccgtgccgg agaactggac gtggatacag agtttatcta taccacccag aaccctacgc
    15361 tctatcgttc ccgccgcatt gcggagcaaa tgctgaaagc gtttgacgag ggaaagctgg
    15421 acgatgtgta tattatctat accgatatgg tttcgtccat tgcctttgaa ccgaaaattt
    15481 tgcagctgct tccgctggat gcagaacact ttgtacagag tgcgggcgag atcaccaagc
    15541 accatgaggc agtgttctcc cccagcccga atatcgttat ggagcatctg actccgaact
    15601 ttatgaaggg catgatctat ggtgccatgg tcgattcgtt ctgtagtgaa cagcaggcga
    15661 gaatgtctgc catggactct gcaaccacca gtgcgaagga tatgttacag gatctgtcgc
    15721 tgcaatataa ccgtgcaaga caggcggcaa tcacgcagga gatcacagag atcgtcagcg
    15781 gtgcaaattc cgccgaattg tgaagacagg ttctaagaac agcacgacac caagcagtgc
    15841 tgtctaaggt aataccgcac aaagagcgtc cggcgacttt gcacgccatc tgcttgcaca
    15901 atttttgtgc ggtattgccc taagaattgg aaggctcggc agcggcggag tcggcgggtg
    15961 tcatctgatg cccgctgtgc cgccgccgtc tggaaaggaa tgatgatatg gaaaaaggga
    16021 aaatcgtgca ggttttagga cctgtcattg atgtggcatt cccgggaaag cttccgatga
    16081 tcaaggatgc attgaccgtc gataacggcg gaaagacctg tgtcatggaa gtggcacagc
    16141 atatgggcaa cggcatcgtc cgctgcatca gcctgtcctc tacggagggc ttcgcaaagg
    16201 atatggaagt caccgccacc ggttcctgca tcaaggtgcc ggtcggtacc gccacactgg
    16261 gcagaatgtt caacgtgctg ggcgaagcga tcgatgatgc cggtgaagtt aagaccgaag
    16321 aaaagtggcc gatccaccgt ccggctccgt cgttccgcga tcagagtccg gtagtcgaga
    16381 ttctggaaac aggtatcaaa gtcattgacc tgctggctcc gtatgcaaag ggcggtaaga
    16441 tcggtctgtt cggcggtgcc ggcgttggta agaccgtcct gattcaggaa ctgatccaca
    16501 acgttgcgac cgaacacggc ggctattcta ttttcaccgg tgtcggcgaa cgttcccgtg
    16561 agggtaatga cctgtggaac gaaatgaaag attccggcgt tatcgaaaag acggctctgg
    16621 tattcggaca gatgaacgaa ccgccggggt cccgtatgcg tgtggcacag actggtctga
    16681 ccatggcaga atacttccgt gatgaaaagc atcaggacgt gctgctgttt atcgataaca
    16741 ttttccgtta tgtacaggca ggttctgaag tttccgccct gctgggacgt atgccgtctg
    16801 ccgtaggcta tcagccgaca ctggcaaacg aagtcggcga actacaggag cgtatcactt
    16861 ctaccaagaa cggttctatc acctctgtac aggcagttta cgtgccggca gatgacctga
    16921 ctgacccggc tccggcgact acattcgcac atctggatgc gacgactgta ttgtcccgta
    16981 agatcgtgga acagggtatt tatccggcag tagatccgct ggaatccacc tcccgtattc
    17041 tggaaccgga cgtagtgggc gaagaacact atcaggtagc cagaaaggtg caggaactgt
    17101 tgcagcggta caaggaattg caggacatta tcgcaattct gggtatggaa gaactggacg
    17161 aggatgacaa gctggcagta tatcgtgccc gtaagatcca gaagttcctc tcacagccgt
    17221 tcaacgtggc agaagtcttt accggtctgc cgggcaagta cgtaccgctg cgggaaacca
    17281 ttcgcggctt taagatgatc gtagagggtg ctatggacga actgccggaa aatgcgttct
    17341 tcaacgtggg cactatcgac gaggcgatcg cgaaagccga gagcatgaag aaggaatcgt
    17401 gagggatgta ctatggcaaa cacatttcag ctggagattc tggcatctga ccgcccgttc
    17461 tatgtaggtg cggcagagca gctggtgttt ccgacagtgg acggtctgat gggcattttg
    17521 ccgggacatg agtccatggt caccattgtc agccccggag aactgaaata tctggtggac
    17581 ggcgaatggc actatgcggc gatcagcgag ggctttgccg aagtgcgtcg ggactatgtg
    17641 ctggtgctgg gtgatgccat cgaactgccg gaagaaatcg atgccaagcg tgccgcagag
    17701 gctgccgagc gtgcgaaaga acggctgctg cataagcaga gcattatgca gtactaccac
    17761 agtcaggctg ccttgaaccg tgcaatgaac cgtctgaaga tctccaagcg acacaatcat
    17821 gaaatttaaa tgcagttttt tgcatgaaaa aacaccgctt ctcagtctgg gaagcggtgt
    17881 ttttatttgc atagtgtgat cgccggaaaa aagtttcgca cgatgtcata ccccagcact
    17941 atcgcaagta caattaccag tgcggccgtc cgccgcggaa gaatctgccg tggtttgcct
    18001 atggcggtgc agaccagttc cgcatagcag cagagcaggg cagcacagag cgtgccgatc
    18061 atggggttgc agccaagcga acggagcacc tgccctttgc atagagccag taccgcacgg
    18121 gtatttccgc aggcgggaca gagccagccg gtggttcggt agaatgtgca gtccggcagc
    18181 ggcagagcct ccagaacgtg cagcagcggg cggtgcaggc agaccagcag cactgctcca
    18241 aggaacagcc cgatgctcca taggcaggcg gtgcggcggc tcattgccgc agaggcacgc
    18301 cggtattgtc gcggaatttg cccagcagaa tgtgaaacag gtctaccagt gttccgatca
    18361 ggaaaaagcc gccggtgcac agccagagca gccctgtgcc gatttttccc acatagaacc
    18421 ggtgcagtcc tgcaatcccg aaaaatccca gaatacacag cagcagagcc ggcgtttttc
    18481 gcttgtcgct ggtaatggtg acgtattgtg ccataatctc aaaacctttc ttgtttttct
    18541 gtatcatacc agatttttcg gggaatgtca agggggagtt gagatttgcg gtgataatta
    18601 cggctttatt atgttagggg acttcttgaa gtagggggcg atgcccgcat cgccccgccg
    18661 gttgactttt ttcaatattg ccgcccctga aaggggaggc ggtggagggg ttctgctgaa
    18721 atcttttact tttgtttcag gtttcacaga agaaatccct ccgtctgccc ttacgggcag
    18781 ccacctccct tttcaaggga ggctattata gaaaaaacat ttttgtgcag tttctaatgc
    18841 tgtcgtgtcg aaaccgtccg gcggaattcg ctcgtacctc gctgtccgcc tagggcatgc
    18901 caacgccgat tgttgacctt gcctgtattg ttaatgttga tattcaagtg ttttccgata
    18961 caggtgggta actttatttc atacggcgtt tgcaagctcg catataagcc tcccttgaaa
    19021 agggtgactc cccacggggt ggggagatgc ccgcaggg
//
LOCUS       DAWBGR010000026        18743 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_998_length_18743_cov_4.551798, whole genome shotgun sequence.
ACCESSION   DAWBGR010000026 DAWBGR010000000
VERSION     DAWBGR010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 18743)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18743)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18743
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_998_length_18743_cov_4.551798"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4095 a         4951 c         5237 g         4460 t
ORIGIN      
        1 tgctgccgcc gtaacattac ccttttcact cttgagattg gggggtaagg tcagcggcga
       61 ctcgccgccc gttaatcatt actagccatg gatcgcataa tagatctaac ggatcaccgc
      121 gataaagctg taagtccgcg tccatgcctg cggcaatgct gcctatgcgg ttctctacac
      181 cgagaatctc cgccgcaccg gcagtaatgg catataaggc atcttcccgc tgcatgccgc
      241 ccttgactgc cagagccgcc gtggtgggca ggtactgaat gggaatcaca gggtggtcgg
      301 tgcatagtgc cagcttgaca cccgctttct gcattataga cggaccggaa atggtgaggt
      361 tctgcatttc cggcttggac cggtcgcaca ggatcggacc ggcaatcacc ggagcattct
      421 cctccgccag aatgtccgag atcaggtgtc cctccgtgcc gtgaataatc acatagtcca
      481 gattgaactc cttggcaatg cgaatggcag tgcagatgtc gtctgcccgg tggcagtgga
      541 agtgtgcttt cacatctcgt tccagcaggg gcagcagtgc ttcggatttg gcatcgtagt
      601 ccggcatgtg ttcgtcggga tcgctctccg caaagtccag ttcctgctga taccgctgtg
      661 ctttcgccag cccctcccgt atcattgccg ctgtcgccat gcgggtgatc ggcgtttcgt
      721 ccctgtcgtt gtagacgctc ttgggattct cgccaagggc aaatttcaca ccggtgccga
      781 tctgcatgcg gtctgccata cgtcctgctg ttttcaggat cgccatggtg ccgccgcagg
      841 cgttggcact gccgggagaa gtcatcaccg tagttacgcc ggctgcacgt gcttcggcaa
      901 aacagtgatc cagcgggttt acgccgtcaa tgacacgcag atgcggggta aaaggatcgg
      961 tggcttcgtt gcagtcgtcc ccctcgaaag caatgccgtt ttcgataatg ccgaggtggg
     1021 tgtgtgcatc gataaagccc ggcagcagca ccgaaccgcc ggcatcaata tcctccggcg
     1081 acagttccgc cggcttgccg gattccacgg cggtgatctt gccgttttct atgcagacat
     1141 agccgttttc cagaatgccg gcatctgtca tcgtatgtat ggtcgcgttc cagattttca
     1201 tgtacgttcc tcttttcgtt tccgtggttt cggcatggaa agactgtagc tttcttccag
     1261 cagccagaac agttccggtt ccggcagtgt gccgtctagc aggacagtga cccagtgtgt
     1321 cttattcatg tggtacgcgg ggaagattcc cgccttttgc agcagcgaac cggtttccag
     1381 cggactgcac ttcacattga gaatatgcac cgttctgcca gacggcagtc ccagcttctc
     1441 cgcgggaata tccgtcagca gcccgtacca tttgccgttt tctctgtgcc gcagaactgc
     1501 cgcgtccggt gtgcttttcc acgggtattc cggcgtggtg ccgaaatttt ccgcggcata
     1561 gcggagaatg ctttcccgtt gatttgtcat attgccgtta ctccagtgat acaatggtca
     1621 cgccgtcctc gccctcgcca aacgtgccaa gacggaaatt cttgacgtat ttcagctgtt
     1681 tcagccgctg gtgaatggct tttcgcagca gtccggtgcc cttgccgtga atgatcgtca
     1741 ccgtggcaat gtgggacatg acggctctgt ccagaaaagc gtccatctga taaacgccct
     1801 cgtcacaggt acagccgcga atatccagtt ccatggtgcc ccgtctggct gcctgagccg
     1861 aaacccgtcc ggcagtctgc cgctgtttct gctggctctt tttcttttcc tccgcaccgg
     1921 caaccagccg cagcttttcc tgcggcacct tggtacgcat ggcaccgatc tgcaaaaaga
     1981 cagtgccgtt cttgtccggc tttccggcaa ctaccgcttt ctgcttggtg tcggtcagca
     2041 tgacggtgtc gcctacctgc aattcccgcg ggagcacata gtcctcgtcc ggcacctcgg
     2101 tcagcgggtt ggcggtttcg tacatcttcc ggaagccccg cttgacgttg cccttggcac
     2161 ggatcacatc gtccccgaaa gtcgcttttt ccttggcttt ccgcagctga tccagttcgg
     2221 agatcatctc gttggactgt gccttggtgg tttccacgat tcgcatggct tccagccgag
     2281 cctgttccat gatgttttcg cggtcggtgc gggtacgctg gacttcttcc cgcagctttg
     2341 ccgcatcttc ttccgcctgc cgcagggctt cctgcatggc actgcggtct tgttccagag
     2401 cctttcgggt ttcttccagc ttggaaaccg cttcttcaaa tcgcagattt tcctgactga
     2461 ccagcgtttc cgccgcggca atgatcgatt ccgacagccc cagccccttg caaatggcaa
     2521 acgcgttgga cttcccggga gagcccagaa tcagccgata ggtggggcgg agagtttcaa
     2581 tgtcgaattc acaggaggcg ttctgcacgt ccggctgctc ggtggcatac agcttcagtt
     2641 cctgatagtg ggtagtgacc atcagctttg ccccgttgtc tttcagccgc tgaatgacag
     2701 cctctgccag agccgcaccc tcgatggggt ctgtaccgga acccaattcg tccagcagca
     2761 ccagcgagtg ccggtctgcc tgttccagaa tctcgatgac acttcgcatg tgtgcagaga
     2821 atgtggaaag gttctgctcg atgctctggg tatcgccgat gtctgccagc acattgttga
     2881 aaacggaaat gcggctgccc tctgccgccg gaatcagcat gccgctcatt gccattaagg
     2941 tcagcagtcc gcaggtcttc agagcaaccg tcttaccgcc ggtattcgga ccggtgataa
     3001 tgagagcctg ataagtatcg cccagcgtga agctgatcgg taccgctttc ttgtagggga
     3061 tcagcggatg acgtgccttt ttcagcagga tcacaccgtc gtctgtcagc gtaggcggaa
     3121 aggcgtgcat ctgttccgcc agacgtgcct tggcgaaata cacgttcagg tctgcacaaa
     3181 cggcaatgtc gcggataatg gtatccgccc atgtgccgca ctcggtacag agttcctcaa
     3241 tgatgcgttc gatttcctct gcctcctgca attccagcag gcgaatgtcg ttgttggcat
     3301 cgaccacagc aatgggttcg atgaaaatgg tctgaccggt ggcggaggtg tcgtgtacca
     3361 gaccggcaat cttgctgcgg tgttccgcac gaaccggcag tacgaaccgt ccgtcacgga
     3421 gcgtgacacg gttgtcttgc agacagtcct gcacctcctg ccgccgcatc atgctgtcca
     3481 gtgtgtcccg cagcttctgg cgggaacgtc cgatcttccg gcgaatttcc gccagagccg
     3541 gactggctgc atcggcaatt tcttcctcgg agagaatgga acgatccagc ttttccagca
     3601 aaaagggcac cggtgccagc tggctgaaca ggtcgtccag tgtggattcc acaccggcac
     3661 agcggtcgta ccagtcactc aaagactgca tctgccgcag gatcgtggcg atttccagca
     3721 ggtctttcag cgaaagcttg gcaccggact ttgctcgccg cagtcccatc cgcatatcct
     3781 gaaaccggtg gaacggcgga gcaccgaact gtatggacag ctgaaaagca tcgtttgtct
     3841 tttccagttc tgcacgggct tccgcgaggt cgggattcgg ggtaatggca agaacttttt
     3901 cccgtgtgat gtcgttggcg gagagattcg ccagcatgtc taagatttta tgaaattcca
     3961 gaatttccag atttttattc atgggagtac tcctgtgata ttggtattgc cgtaaaggca
     4021 aaaatccgca gttactctgc ggacgggttc acgatcgatc gataaaaggt cagagccttg
     4081 ggctttagcg acaggtgata ctggacgaaa cgctccgtac agatgcccag catctgcaaa
     4141 ttctctgccc ccagctgaaa gtggaacagc cggttataat ccgcaaagag aatgtgccgc
     4201 atcgcctgta ataccggtgc acggagcaga agcggttcct gcggggaatc cggtgcaccg
     4261 cagtcggtac agaaaaaaca gcccttttcc acggagaaat agagccgttt tggtaaaaat
     4321 accccacagc cgcggcacat gagcaggtcg ggcatcatac ccagctctgt catcagccgc
     4381 agttcaaaaa tgggttttaa taatgcttcg ctgcgttgtc cgctttccag atagtggagc
     4441 gtgttcagca tcagccgcat gacgaagcag ttttcttccg gtgtctggtg ttcccgcaca
     4501 ctaagccgga tcaactcgcc gaaatacatg gcgaggctca gacgggagag ggattcccgc
     4561 agccggtaga agatgcggat cggttcggca ctgtccagat accactgatt ccggcgtttc
     4621 tgtacgctga aggtggcata ggaaaacagc tgtgtcgcag aaagtcccgc agaactgttc
     4681 tttttcgccc cgcggacata gatctcctgt actgtgttgt gctctgtcag cacatcaatg
     4741 aacttcccct gttctccggc actgcgttcc gccagaacca gtccccgata gatctccatg
     4801 tgtctgcttc tcctttgctg cgggtgcgtt ctgcggcttg gcgttcatgc cctgccgata
     4861 gtgcagatag tcgtcaatgt gtccggtcat gcaaaaaata tcccatgcgt ccatatctca
     4921 ggttcctcct gtttcttcgg atagagatag gatttccgga atcgtgcaat ttatgcgtga
     4981 gagaacgaac aggcaggtta ctccggattg tccagaccga aattgtggat cagtccttcc
     5041 ttgttccgcc agtcctcctt gaccttgacc cagcactgta aattcacctg aatatagaag
     5101 aaccgttcca gatcctttcg ggcgagggtg gcgattttcc ggagcatggt gccgtttttg
     5161 ccgatcagga tccccttgtg ggattcccgt tcgcagtaga tcgtggcatc aatgtccagc
     5221 aagtccttgt cgtcccgttc cttcatgcgt tcaatggaaa ccgcaatgcc gtggggcact
     5281 tcgtcccgca gcaggttcag gatcttttcc cgaatcatct ctgccgccag cacccgttcc
     5341 ggctggtcgg tgaatttttc gtcggggaag tagtggggag ccggctgtgc cagtgccatg
     5401 acttcctgtt ccacaaggtc gatgccgtcg gcttctgtga cgctgacagg aacagtcgcc
     5461 gaaaacgggt gtgccgcatt cagcctggca atggtggcca tcagcttgga cttgtcgctg
     5521 caaaggtcga tcttgttcag caccagaatg gtgggcgttt ttgccttttc cagcgaacgg
     5581 agcagttcct cctcctgctc ggagagaggc ttggtgcagt cctgtacaaa cagtaccgcg
     5641 tcaatgtctg ccaccgcatc tttcaccgct ttcagcatgt gcccgctgag cttggtcgtg
     5701 ggcttgtgca gaccaggggt atccatgaaa accatttgcg tttccccgac tgtcttgatg
     5761 ccggtgatgc gggtgcgtgt cgtctggggc ttcgggctga ccgaagcgat cttttccccg
     5821 ataatggcgt tgagtaggga ggactttccg gcgttggtgt gtccggcgag cgtcacaaaa
     5881 acagattttg tcatgcgtca gtatcctccg gctgattggt gaaggtggct tctctggaaa
     5941 tgcccagttc gcccagtact tcttcttctt tttcccgcat cttccgttct tccagcgggc
     6001 tggtttcgtg gtcgtatccc agcaggtgca gcatggaatg cactgtcagg aagccgattt
     6061 cccgttccag cgagtgtccg tacatgtttg cctgcttgac tgccatttcc agtgaaatga
     6121 ccacatcgcc cagcagtgca cagccggttt cctgattggt gtcatagtgc ccgtcctgtc
     6181 ccagtgggaa cgagagcacg tcggtgccac ggtctttttg ccgatagatc cggttcagtc
     6241 tgcggatctc tgcattgctg acgaaggaga cactgacttc tgcgtcctgc ccgaaatgtt
     6301 ctgccaccag aaccgcctga cagcagcgac gaatgagcat acggattcct acaggcaccc
     6361 gcacttcggt ttgattattt tttaccatga cttttacttt tggcatgatg aatgatctcc
     6421 tttcgtgaga ttttatatat aaaatctttc tatgcaatgt aaatcagatc ttttctatac
     6481 gggcaggtgt ttctgcaaag caggggacag gctgtgccct gtcctttgcc acagaagcat
     6541 acgctgcctt aaattttcgg caataccgca caaagattgt gcgacagatg ctctttgtgc
     6601 ggtattgcct ttcaaattca gtgttttccg gcatctttcc ggtcggtgtg ttccggcttc
     6661 cggtgggctt gcttttgttc tgccgcttcg taggcacgga taatgtcctg taccagcttg
     6721 tggcggacaa catcgcggtt ggtgaactcg cagatcgcaa tgtcattgac ctttcgcaga
     6781 atgcgggagg cgatgaccag accgcttttc tgcttgtccg gcaggtcgat ctgcgtggtg
     6841 tcgccggtga tcaccatgcg gctgccgtcg cccagacggg tgaggaacat cttcatctgt
     6901 tcgccggtgg tgttctgtgc ctcgtccaga atgataaaag cgttgtccag cgttctgcca
     6961 cgcatgtacg ccagcggggc aacttcaata gagccccgtt ccagatgccg ctggaacgct
     7021 tctgctccaa gcatttcaaa caagccgtcg tacagcggac gcagataggg gtctaccttg
     7081 tcctgtagat cgccggggag aaatcccagt ttttcgccag cttccaccgc agggcgggtg
     7141 agaatgatcc ggttgacctc gtgccggcgg aatgcccgca ctgccatagc cactgccaga
     7201 taggtcttgc cggtgccggc aggccccagc ccgaatacga ttgtattttc ggcaatggca
     7261 cggacataac tccgctgtcc cacggtgcgg gtgtgcaccg gttttccggc ggcagtcaga
     7321 cagacggtgt catcatctgc cagttccgcc agctgtccgg cagagccggc atcgaccata
     7381 tcggaaacat agtgaatgga ctggggcgtg atcgcctgtc cgttggagat catttgcagc
     7441 agagccgtga ctgcaccggc ggcaagttcc acctgtgcac cgtccccctg tatccgcagt
     7501 tcttctccgc ggcagatcat acgaacgtga taccgctgct ccagcagctt gaggttttcg
     7561 tcaaagctgc cgcataatgt ctgaaattgg tttgtgtctt gtattgagat cagtcgttct
     7621 gccattccgg aatccgttcc tttccccagt ttgctacagc ggcaacatcg cacaagtttt
     7681 gtgcggtgct gcctatcctt tcctattata gcataatctt tgtgaaatgt gaaatggtat
     7741 tttgttgttt taacaaaaaa ttcaggaatc actgcaaaaa gcagcaacgc ctgaattgaa
     7801 aagcctattt tagtagaacc ggtattcgga aatatctgac aaaaagatct ggtataagga
     7861 gaacattccg tcgtccagtg tggtgcggct gctctccagg gacaggaacg atgcatgttc
     7921 atacagcgga aaccgcaggg tgtactgcca catttcctcc gcggcggaaa acagaacggc
     7981 ggcatgatac gtgcgacaga atttttgcac gatctgttcc tccggagagg gaaagtgtgt
     8041 gtcatagaga tgttccgtct ggctgtgccg gtgtgccggc ggctcggtgc cgtcagaaac
     8101 tgccgtgcac cgcagcacta cctgatcgcc ctgctgttcc gtctgccacg tcaggcggga
     8161 gatctccgga cagtgatccc gcagatacag aaccatgcag agcagacaga tctgtaaccg
     8221 ccgccgattg gcgaaaatgc agagcggttt ttcggcatcg gctgtcattt ttagtgcttt
     8281 tcccagtacc atacgacacg attcgacaaa attcgacaat tcgcggttca gaaaaagagc
     8341 ttcgcccgaa agctcgtttt tttgcacata tttcagctgt tccagccaga gcacagaagg
     8401 acgcatcagc cggcagcagt tgccctgaat gatgttcagc tgttccattt gttcttccag
     8461 caccatgcgg gggatctggt cgccgttttc ctccagcgtc tgatacagcc cgctgacggc
     8521 gttggaaatg ccgaagatct tctgccgcat ggcagcgatc cggttttccg tttcatgctg
     8581 cttgtcggta tcgctccaga gaagatcgtc tgccggctgg ctgtggatct gcaccagtat
     8641 tgccggtttt tcgccgtact gccgtggctc ctgccggacg cagtacagac tgccgccctg
     8701 ccggagcaga aacatgccgt cctccggtgc accgttttta tgatagcggg gcaaagagct
     8761 gttctgcggt ttctgtcgga ctgccgcggc atcgttctgc cagagcagcg tgccgtccgg
     8821 ggcataaaaa agaatggcgt cttcagactg ctgacaccat ttctggatct gctgtagttc
     8881 ctcttgctgc atgggcgttc tctcctcctc actgcatgat gcttttgtca ttattctatg
     8941 ctattattat actacatatt tcagaatagg caaataaaaa aaatgataat tttgtccact
     9001 ttttaatttt tgtgcagttt tcacaaaata tgctttcctt ttttgcggaa aacgtggaaa
     9061 ataagagaac ttgctatcct tgcccctgct ttttttgcga aagtgcttga attcttctcc
     9121 gaaatggtgt aaaataagag tatcaaggaa tccgctggga gaaaatcggg cttcggtccg
     9181 gcaattccgc cggtgttttc ctaaaaacta ggaggtaatt tccaatgtca gttaaagttg
     9241 ctattaatgg ttttggccgt atcggtcgtc tcgcattccg ccagatgttt ggtgcagaag
     9301 gctatgaggt tgttgcaatc aacgacctga ctaagccgtc catgctggct cacctgctga
     9361 agtatgatac cgcacagggc ggttatgcag gcaagatcgg cgagaacaag cacactgttt
     9421 ctgctgatga cgaagctggt actatcactg tagacggcaa gaccctgaag atctatgcaa
     9481 aggctaacgc tgcagagctg ccgtggggcg agatcggtgt tgacgttgtt ctggaatgca
     9541 ctggtttcta cacttctaag gctaaggcac aggctcatat cgatgctggt gcaaagaagg
     9601 ttgttatctc tgctccggct ggtaacgatc tgccgactat cgtttacaat gtaaaccaca
     9661 acactctgac tgctgatgac aagatcatct ccgcagcttc ctgcaccacc aactgcctgg
     9721 ctccgatggc taaggctctg aatgactatg ctccgatcca gagcggtatc atgagcacca
     9781 ttcacgctta caccggcgac cagatgatcc tggacggccc gcacagaaag ggtgacctga
     9841 gaagagcaag agctggtgct gcaaacatcg ttccgaactc caccggtgct gctaaggcaa
     9901 tcggtctggt tattccggaa ctgaacggca agctgatcgg ttctgcacag cgtgtaccgg
     9961 ttccgactgg ttccaccact atcctgactg ctgttgtcaa gggtgcagac gtaactgttg
    10021 acggcatcaa cgctgctatg aaggctgctg ctactgagtc ctatggctac aacgaagaac
    10081 cgatcgtttc ttctgacgtt atcggcatga agtacggttc tctgttcgat gcaactcaga
    10141 ccatggtttc caagatcgct gatgatctgt atgaagtaca ggttgtttct tggtacgaca
    10201 acgagaacag ctacaccagc cagatggttc gcaccatcaa gttcttcgct gaactggcat
    10261 aagttcgcgt agcgttctga acggacaggt ttctgttcgt taccacattg caaaagaagc
    10321 ggcatggcgg tttcgtcatg ccgcttttta tatgccctgt cggtgttccc tgaaaaaatc
    10381 tgcaaaaaat gattgacgta tctgtttcca atatggtata ataaaaatga gaaaatgaag
    10441 cactgtgtaa agcctgctga caggtttttc acagcgttga caaagcattg ggacagaaag
    10501 gcaggtgagg gatccttgcg aaagatcgga cggaaaagtt tgttcgtttt gctggcgttg
    10561 ctgctggtga tcggctgctt ttccggtatg tgtccggttt cagccgcggg aagtgcggat
    10621 ctgaagatct atcaggacac catttattac acggtttctg cgatgcacga ggtgacgatc
    10681 aaaagtgccc gttcagcagt gaccgaagcg gtgataccgg cggaactgga cggctgtccg
    10741 gtgacagaga tcggcgagta cgcgtttaaa gactgcacgc ggctgaagcg ggtggttttg
    10801 ccggacacga ttcgccggat cggggaattc gcctttaagg actgttcccg cctgacagaa
    10861 ctgtccattc cggatacggt ttccgagatc ggctggggta ttgtgcaggg tacttcgtgg
    10921 ctggaaaacc agaccagcga tttcgtatca gccggacaag ggatcctgct ggcgtacacc
    10981 ggcacggaaa aagatgtgac tgttccggat accgtgcgtg cggtcggcgg ctatgccttt
    11041 gacggctgca ccacggtgga aacggtacag ctgccgtctt cgctgcgttc gatcgatgcg
    11101 tttgcatttt ccaattgcat caacctgcgg caggtgcaga tcgcgaacgg gctggaatcc
    11161 atcggcgaat atgcgtttca ctggtgcgtt tcgctggaac agatcgaact gccggacagc
    11221 gtgaaaaacg tcggcggtca tggattcagc tattgccggt cgctgcggaa agtgcgtctt
    11281 tcgcaggcaa tgacacagat cagcaacact ctgtttcagg ggtgcagcag tctgacggag
    11341 atcgagcttc ccgaaaacat caaaacgatc tacaactatg ccttcgacgg ctgtgccgca
    11401 ctgcaaaaga tcgtgctgcc ggcagcagtg gaggagatcg gagccagtgc cttttccggc
    11461 tgcggcagtc tggaacagct ggtgatcctc aatccggcgt gccgtatcta tgacacggaa
    11521 caaacggtaa gttcaagcac ctgtatcaat ggctttgcga attccactgc ggagatctac
    11581 gctcggaaat acagccggca gttccggccg atcgaccggc agcgtggcga tatggacggt
    11641 gacggcagtc tggacacatc ggatctgttt ttgctgctgt atctctgtgc ctgtcgcgga
    11701 gccggtgtgc agacaaatcc caccagtgtg gagctgtact gtgcggatta taatgcagac
    11761 ggcaaaattg acaatctcga tgtctacgac ctgatgatgc atctggcaaa ggttggtgcg
    11821 ggattggcgg aaaaatgatt tttatgaaaa agaatggatc gcccccttgc gaatgttggg
    11881 ggcaaacagg ttgaaaagaa aggaagtgtt ccgcaatgcg gaaaaatcgt tggagaaggt
    11941 ggaccgcgtt cctgctggca gtgtgcataa gcatttctct ggtactcggg acagcacagg
    12001 ggataggtgc tgccgcagaa tccagccaat cgcaggaaca attattcaac ggtatctatt
    12061 atcagacaga tgatgcggga aacgctgtga ttaccggcat ggaagggaat ttttctgtgc
    12121 tggacattcc gtccagtatc gacgggtatc cggtgactgc aatttccgaa tcggcatttc
    12181 aacagaaaac cgccctgaaa agcgtgacga taccggacag cgtgaccacc ataggcagca
    12241 gtgcatttcg tggctgtacg gctttgaaaa cgataaaggg cggcagcggg atcaccagtg
    12301 ccggctcctg tatcctggag gggaccgcgt ggctgcaaga ccagactgat gatgtggcag
    12361 tgctctccga cagagtggca gtttccgcaa aaatcggact gaaaaccgca gtggtgccgg
    12421 acggtgtggc agtgctctgc aatacgctgt tcagcgatca gactgctctg acggaagtgc
    12481 agcttcccgc cagcctgacg tatatcggca aaggtgcatt tatcggttgt aacgctttgg
    12541 aaaagatctt tttgccaaat caggtgcagt ctgttggtgt ttcggcattt cgccactgca
    12601 ccagcctgaa aagcattcag ctgccggatg agctgctctt tgttggctcc ggagcatttc
    12661 agagctgttc tgcactggaa accgtacagt tcaacgaaaa attacaggtg atctcccaga
    12721 gtgcctttca ggactgcacg gctctgcggc tggcagtttt gccggaatcg ctgcagctgc
    12781 tggaaagcag tgcatttcag ggctgtactt ctttgagcat ggcggtgctt gctggcagta
    12841 cgttgccgga gattccggaa aatgcgttca gcggctgttc catgctggaa tatgtcacaa
    12901 tgagcaattc tgtcaaaaag atcggggcaa gtgcgttcag caactgcatc aatctgcgga
    12961 gcatgacgtt ttcccctgca ctgactgaga tcggggaata tgcattttcc atgtgtacta
    13021 gtctgaaaaa tctgaccata ccggaaacgg tggaaactgt gggaaaagat gcctttttca
    13081 attcaggctg gagtttgcag cagtccagca gctggaagat ctgtgacggt gtacttctgg
    13141 aatactgcgg agaagccagc gagatcgtca ttccgcctac ggtgcggctg atcgctacag
    13201 gatttttgtg cagtgatgcg gctccggttt cggtcacgct gccctatggc atgataagag
    13261 tggcagacgg agcgtttcag ggctgtgaaa ccatagaaaa ggtgatcttc tcagacagcg
    13321 tgacagagat cggcaatgcc gcatttcagg actgcacctc gttgaaaaac ctagtggaca
    13381 tgaacgccat acagacgatc ggcgacaatg cctttgaggg ctgcaccagt ctggtgaatg
    13441 tagtgctgcc ggatacgctg acaaagctgg gcgtggcggc gtttcgcagc tgctccagtc
    13501 tgactgctgt gaaatttccg gtaaatctga cggaactgcc ggaggcggtg ttctataaat
    13561 gcgttgcact cagcacggaa aacggcagca tggatctgtc agattcgtcc ctgcaaatcg
    13621 ttggcgatga tgcattcggc ggctgtgaga atctggacaa tctgacattt ccggcatgct
    13681 tcaccaccct ttcggcgaat gcgttttacg cgtatgtgca caagcagaga acggtgacat
    13741 tctccggcaa tgcctgcacg ctgcctgacg aagccaacat ctttccgcgg gatacggttc
    13801 tgagagggca gaagaactct ccggcacaga cctatgcgga aaagtacagt ctggaatttc
    13861 aggcactccc ggatgaaact cccgcttcga ccacgacagc caggacaacg acaacaatag
    13921 tagaacacac caccaagaga agcaccagca aaatcgtagc aactgccgaa gccaccacaa
    13981 agacaacgcc tgtgaccaca gtgaccctgc cgccgtatat cacggtgccc accacactgc
    14041 cgaccacgac aacggaaagc agcactacgg aatggaaaac cataccgatt cccaccggca
    14101 cgaccgcagc agcttctgca aataccacca cggaaaatgt taccacgaca accacggtgt
    14161 ctactgctcc ggtaccggtg cggtatatca agggtgacgt ggacagaaac gccagcatcg
    14221 acagcaccga cctgttcctg atcctgtacg ccagtgcccg catcggtgcc ggctatccga
    14281 tcctgacgga cggcacgctg agcgattggg agatcaaaag tatggacgta aacggtgacg
    14341 gtaccattgc ggcagacgat gcatatgcgg tgctgctgta ctgcggattg aagtccgtgg
    14401 gaaaacaccc cacgtcgctg gacgattttg attgggaaaa caacaccatt tatacaggct
    14461 aaaataggat attccaaagg aaacaagaga aacgctgcat aaagtcgaat tatcagcttt
    14521 atgcggcgtt ttctcggaat ggagaaaaaa catgcagtat caaaacccga ttttatccgg
    14581 atttcaccca gaccccagca tttgccgcgt gggaaaagat ttctatctgg tgaccagttc
    14641 tttcgagtat ttccccggga ttccgatttt tcacagtcgg gatctggtgc actgggagca
    14701 gatcggacat tgcctgacgc gaaactcgca ggtgcatctg gtgacaggag cacccaactg
    14761 tctgaacatc tacgctccga cgatccggta tcacgagggg acgttctatg ttgtggtgac
    14821 caatgtcacc ggcgacaatc acggcaactt tatcgttacg gcaaccgatc ccgcaggcga
    14881 gtggtccgaa ccgattcagc tgccctttgc tggaatcgac ccgtccctgt tctttgacga
    14941 tgacggcaag gtgtactatc tgggcacaga cggcggcatt tatctaagcc agctggacat
    15001 tgccaccgga gaagccattg gcgaaaccca cgccctctgg cagggcaccg ccaacaatcc
    15061 ggaggggccg catctgtaca agatcaacgg catgtactac ttgctgctgg cacagggcgg
    15121 cacggagctt tgccacatgg cagttctggc acggagcaag tccattctgg ggccctatga
    15181 gccgtgcccg cacaatccga tcctgaccaa tatcggcaaa aacctgccca ttaaggcagc
    15241 tggtcacgcc gatctggtgg acgatgccga cgggaactgg tgggcagttt gtctgggcaa
    15301 tcgtccgctg gcgtatccgt tccgccacaa tctgggacgg gaaaccatgc tggtgccggc
    15361 tcgtctggaa aacggctggt tctatctggg cgaggaacgt gcagtgctgc cggaattcac
    15421 agtagagcac gcccccgcag aggagattcc cgcaggctat gcggcatgca gtgcggtgac
    15481 agatgatttc cgcggtgaca gtctgcacct gatgtggaat tacatctata atccggtgga
    15541 gggcttgata cagcaaaccg catctggctt acagatgcac ggcaacgagg tttccctctg
    15601 ggaagatgcc cccaaggcgt ggctgggacg acggcaggaa cacttctgct gcaccgccga
    15661 agcagacctg cattttgtgc cgcagcagga gggggaagaa gccggtctga ctgtgtacat
    15721 gaatccggca catcactatg agattgcccg caccatgcgg gacggcaggg actgtgtgat
    15781 tttgcggcgg cggatcggcg gactgtgtgc cgtagagcag gtgctgcagt acagcgatga
    15841 aaccatgacc ctgcggctgg aatgcgatcg ggatcgttac cggttctcgt tccgcacggc
    15901 ggacggcaca gtgcagcctg taggctccgg tgaaacccag tacctgacca cagaggcagg
    15961 cggctgtttt accggcaact acattgccct gtacgcttcc ggaaacggca agccgatgac
    16021 gaccactgcg acctttcccc gtttctctta ccagccggaa gcagaataat cacttgcttc
    16081 tacatctaca gtacaccatg cagatatgct gtatggtgta tttttttggc atgaatgcgg
    16141 caaagcgggg catactttcc ttgggaggaa agtgcgatgc gaaaactgaa aaatcgtctg
    16201 gcagtgctgc ggctgctgtt ggtgctgctg ttttgtacgg ttgccgtata tctggcgtgg
    16261 ctgatgcaga agccggaact cttgcaggct gccgccggac agggcagcta cacctgtacc
    16321 gccggaacag ctgccggcac gatctacgac cggaacggtg tgccgctggt caataccagg
    16381 tcggtctgca ccgcagtcgt cagccctacg ccggaggcgg cggaggctct gctgccccat
    16441 gtactggacg cggaagcgtt ttacgaaaag ctggcacagg ggaagccctt tacctgtacg
    16501 gtggacacag cggacattgc ctgtccggac gtgactgtgc tggagattcc ccagcggtac
    16561 accacgcccc agccggcaca gcatgtcatt ggctatacgc aggaggggaa aggcgttgcc
    16621 ggactggaat ctgcctacga tgcggtctta cgcggcgtgg actcccagtg gagcgtgact
    16681 ttttccgtgg acggcaaagg aaagccgctt gccggcgaag cggctcaggt ccgttacggt
    16741 gccaatccgg tgcagggcgt gatgacaacg ctggacattc ggatgcagcg aatctgtgaa
    16801 actgccggtg catcactgca aaagggctgt gtggtagtca tggacgtgga aagcggggac
    16861 attctggggc ttgccagctt tcccggctac atgcccgatg cactgggaga agccatgcag
    16921 gatccggaca gccccatgat ccagcgtgcc ctgtacgcct atccggtggg gtcgatcttc
    16981 aagctggtga cggcagcctg tgcctacgag aacggtgcgg aaacggcgtt tcagtggaac
    17041 tgcaacgggg cgatttccat tggctcccag cggtttcgct gccacgaccc gcaggggcac
    17101 ggactgcaaa atctggcaga tgccatgcga aattcctgca acccctattt tatcgcactg
    17161 gggcagacgt tttccggaaa ggaactgctg gaaacggcaa aggctctggg cttcgggacg
    17221 gaaacggtgc tgacctccaa catgatcggc tccagcggga cactgccgac attgcagcag
    17281 ctgaaactgc cggcggagca ggcgaacttt tcgttcggac agggcgttct cacggcgaca
    17341 cccctgcaaa tcacccgtat gacctgtgcc attgccggaa acggtaccct gccggctgtg
    17401 cggctggtgc gtggcgtgac ggaaaacggg cggacagtgc tgcgggagga aacgggactt
    17461 tctgaaaccg gcatttctgc ggacacggca cagtttctgc ggcggctcat gtgctacacg
    17521 gcggcggacg aggattttca gggcatgcct gcccatgttt ccatgggagc aaaaacctct
    17581 acggcacaga ccggcagggt ggacgaatcc ggagacgaat actgccatgg ctgggtgacg
    17641 gcgttttttc cggcggactg cccgcagtat gccgtgacgg tactggcgga ggacggcggc
    17701 tatggcaacc agactgccgc accggtgctc cggcagatcg cggcggcgat catgaggctt
    17761 tgaacattct cttgggcaat accgccagac gctctttgtg cggtattgcc ctggtgaaat
    17821 tgtccggttc gtctattgac acgccgtgga aaatcgtgta taatagaagc agggaattgt
    17881 tcccgaaaca aacgccgcta tggcagattg gagttttacc catggcagat gaaaacatga
    17941 tggatatgga tatgcagggc atgctgtata ctctggaaga cgaagaagga aacgagcagg
    18001 aattcgaggt tctgggcgaa ctggaatatg aagatgcaac atactgtgca ctggttccgt
    18061 accatgaaaa tgaagaagac atgctgaaag acgacggcga atttgtcatt ctgaagcggg
    18121 aaaccattga cggagaagaa atgctctgca ccattgagga cgatgacgaa tatgatgcag
    18181 taggcaacat gttcctccag tatctgaacg acatgttcga cgaggaggac gaagaggaag
    18241 cgtaagcact tccggttgac ggtagaaaaa ttttcacaaa tttcctattg atttttggga
    18301 aagatagtgc tataataaac atagaacgaa accggaagaa gcattccggt tggcattact
    18361 gcccagtgcg agacattgcg gtttttataa tccaacactc ctttataggg aatctgactc
    18421 cgtctgtgga ttgcagacct cctgcttgcg gcaatgaccg taacggacga agggagcgtg
    18481 aagcagaagg gaagataccc accttgctag atatagcttg gttttattcg ctgcaagacg
    18541 gctgggtggt tttttgaaat cacaaggcac tctgtgagag atcacggagt gccttttctt
    18601 tttccgcgag atgtttccac atggaaatca attttcaaaa atatgttgat ttaaattgtg
    18661 ccaaattatg aagtttgaca agtaaggctc ccctgaaagg gcacccgtag ggcgtgcctt
    18721 gtgctgagct ggcagtgcgt agc
//
LOCUS       DAWBGR010000027        18703 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1002_length_18703_cov_3.093844, whole genome shotgun sequence.
ACCESSION   DAWBGR010000027 DAWBGR010000000
VERSION     DAWBGR010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 18703)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18703)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18703
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1002_length_18703_cov_3.093844"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2407..2416
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7082..7091
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11382..11391
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13391..13400
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15173..15182
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4468 a         4418 c         3894 g         5873 t
ORIGIN      
        1 tgtaccaagc tgtgtaatgt cataatcctt agaaacatag ttttcaatga catttgccat
       61 gttgtttcgt gcagcaatct caatagagga agaaagagcc tgtatctgct gattatcaag
      121 ccccatattc tgcaagtttt caagaatttg attttcacag atcgtctgac ggagaattgc
      181 ctgaaaatgc ggtgcattct ctttttctgt ggtaaacata actttgccgt cacgaatcag
      241 tcctgaaaat tctgcaccac gctcgtaaag cgtcggttca atcttatgat acttgtcagg
      301 tgtcaaggga gaaatcataa actgtcggtt tcggatttct ctgtatggtt tgttaccgat
      361 gatttcttgt gaagcctgtc ggctattctg aatcattggt tttctcatag cctttacggc
      421 atcctgaata gcacttatct gagaaatatc agcctgagat acagtcatta cccctttgcc
      481 gctgggataa attcttgctg aaaacgaaat gttcgctttg tttgccagtt ccgccatttt
      541 gagaatggtt tctctgtcct cgctgatata ggatttctga ggaatatatt tgtagtctgt
      601 attgccgata atatttttat cctgcggaat atgttctttg cccgacttct gcaatctgca
      661 aagggattca tcgagacctg taacattctt gaagtggtca atttcagaac tgtttaccgc
      721 aatcatcgtg ttgctgccat cgctgtatgc acagtaattc agccccgatt tatcaagtgc
      781 ctgctgtatc tgctgaaatg tatcttcacg catgactgca acggttttat ctctgttatc
      841 cgttgcttcc tgccaataag gattgccgta tcgcttcatt ttgttcattc tcccttgtaa
      901 tatgtcattc caatcttttg ctccatttgg tgttactctt ttcatggcag gatactgagc
      961 aatcagtttt tcggcaaatt cgtttcctgc cttatcgtta tcggtgcaga gatagatgtt
     1021 ttcaggtgat attgcatatc gttgcatcgt ttccgtgaca acagaaggtt ttacacctgc
     1081 catggaaacc aacctgtaat cactttgttc agagtgcaga tcagcaaatg agatcgcatc
     1141 aatcgcactc tcgaaaaaat aggtgctttt gggttcaccg atgaccattt caaaaccgta
     1201 accgtctgct gcccctttgt caaaggattt gaaccgtaca cttactgacg taccgacctt
     1261 ttccgcaccg accagtaagc cgctctcatc gaagatcttg aatattgcat ttcctgtttt
     1321 ttcttcctgt gataatcttc catcagtgat cagatcagca attgcatcgt aggacagacc
     1381 acgaatatta tgcagatagt ctgtgatacg ggaaatttcc ttgctgtcat gaaccgtgat
     1441 ttcacgcttt ttcggtggtt caactcttga tgtactgtga gagtgaaacg aaactgcttt
     1501 ttcatcgctt aaaagttcca ccgaagaaat aaagtcaagt cccataaatt cctgcacaaa
     1561 ctgaatgttg tttccgcccc tgttttccga gaagcgaaac cattttccaa cttctccggc
     1621 tttgttatct ttgatgtgca ggctgtcatg ttcttgcaga acgtactctc tgccgacctg
     1681 ttttaaggaa aatccctgac ttctcaggaa atcagggaga ttgacaagat tggcacgttc
     1741 gatctgttcc ggcgtaactc gcatcaaagt tcctgccctt tgcttttaga ttccctttgt
     1801 ttcggacttt ctgccttcac ttcctgtgca agctttttca tcttctttcg gctgaaataa
     1861 gccggtgctt ctgattttct tacggttacg gcagatttgt caccgtccaa tcttgcagag
     1921 tagggaatgc cgtccttttc aagcttttgc atggtttcct gtgcgtcttt ttccgacatt
     1981 ggatggattg ctctgtcctc tctgggaatc tgtcggaaca cttctggatt tacaagacga
     2041 acgtgtgcat ccttcgcttt ttcctccgca gcgtggaaag caccctcatt tttcttggaa
     2101 acggtaatgg taacaccttt ctccgtttcc acagctgaaa atgggatctt ctgcttttcc
     2161 aattctgtca ttatgattgc acccaccttt gccggttcgt taataatcac tctgtcctcc
     2221 ggcggaatct gcctgtaata gttgggattg atttttcgta ctcccgaacg ggatctctgc
     2281 ggttctttct tttccggtgc aggtttttcc atctgttttt ccggctgtga tttcatgaca
     2341 gttttcgcaa acagatttac cagtcctgca tgggaactga gggaaagact tcgttttgtg
     2401 tagagtnnnn nnnnnngtgt agagttccgg tacgttctga agcgtatctc ttgcaaagtc
     2461 cttgacttct tttgcgattc tgccgtcact ggtaccgttc aacgcacagg caagaacaaa
     2521 ggcgatacgg tcatcagaaa atttctcacg cacgtcactt agtgcctgtt cagaattcag
     2581 ggtatatcgc tcgtaatcat aattctggga aagtgcctgt gagatttcct ttgcacaatc
     2641 catattagcc tttgccgatt ctcggaagga ttgcagtgtg gcatcatcac aagtgctaaa
     2701 atcaatttct tccagaaata acggcacatc ggaacgttct tcctttttgc cattgttgat
     2761 gattccatca atcatgttca tatcatcttc catagacatt tcagcattgc gaagatattc
     2821 agcagaatca agcatcacgg tttcaatcgg tttgtcaaaa tcagacaaat cgccatttga
     2881 caaaaagtcc gtattttcaa tttcagcttc tgtttgtgca atggctttgt caacaatatg
     2941 atcgtttgca ttttccgttt caggagcatt ttcaagctta tggattttcg cttctacagc
     3001 agagatactt gattttactg cttcgatttt ttgaccaagc ttgaattttt ctgccatgga
     3061 atcacttttt tcatactgtt caaacagttt ggaaagcttg ctcttttctg tatccatgct
     3121 gatacgcagc agacctttgc tgctgttccg caaattttcc atggattcct tgaacagttc
     3181 tctgcgtttc tgcggatttg gtatcatgaa cgactgaatg acgttgctga aacttctgag
     3241 tttttcggct ttcagcctga aaacagcgat acggtcatcc agtttggaaa ttttgttttc
     3301 tgcctttttg atgctgtttt caatcttcgg aatacggttt tcatggattg cattgatttt
     3361 tgtttcattt gctctgatga tcgcagatac agcaccggga agtttaccgc tgtccattaa
     3421 gtcctgcagc atcgcatttg tcgcttcaag tcttgcagct ttttcttcga gcaccgcaat
     3481 tctgttttcg ctcttgctga tctgatcctg tgtttcagct ttctgttcct gaagatgatc
     3541 cagtgtgtcc atatggctgc cgtaagccat atgaaaaatc ggcagcagcg tttcactctt
     3601 gtgtgcatct gcttcaagct ccggctgttt tgtaacttcg ttatcttgta tcggcattta
     3661 tgtcacccct cgaagaatat atttatatga ttcatctcgt tcgctccatt tcacgatttt
     3721 tctgctttat attttcctga ttctcaattt cagcttctct tgaagatttg tcatctacat
     3781 acaaatcctg ttccagtgct ttctttatgc tgcaattatc gctgtgccaa cagttgatat
     3841 aggtttgtgc aagcatgatc tgaatgtcaa tcggcttgtt ttccaattgt gcgaccgtta
     3901 cgcccaaaac cacagcaatt tcaggaagca acagccgtgc atagtactta ctgtccggtt
     3961 cagacaaaaa gagcagcatt tcttctgtat atggcttcat cgctcattga ccattctgac
     4021 aagtttgtct ttctccgtgc cgatcgcata ctcatacatg agattcagct tttcattttc
     4081 agaaagcggc atcagactgc tgatctccat gcctgtattt tctgcaatct cacgcatacc
     4141 gagaatgagt gttccttttc gccacagctt ttcgatttct tcatactgct tatgtgccga
     4201 aagttcgtca cttgcttcat agcttttcat ggcatcgcat atttcagcct gtatttcttc
     4261 cggaaatgaa tagaggctgt cagtgagaac gccgagaaca tctgccgctt ttcttaatgt
     4321 ttctatatcc aaaattcgtt cctttctgac aggtcagatt ttccagacgc tgtctggaaa
     4381 atgcaaatca ttcttttctt gtcgaccaat tgtaatacgt cttgccgtta aacgcatttt
     4441 ccgctacttt tggcacaaca atggtctttc tgccgctttt gatatatgac gtttctttac
     4501 cggaaagcag agatttcaac tgcccctctg aaagttccac gccatatact ttggtaagat
     4561 tcattccgca tcgtgtggaa cagtaccagc catacttccc cttgataacc ggcttgccgc
     4621 actttggaca atttccgata ctcggctgat tttcgccaaa agacacggaa tcatcaacag
     4681 aaccgtattt acttacaatc tccgtcacaa agcggttgat ttcattcatg aaactgtccg
     4741 cagaaagact gccatgttcc acttcaagca gcttgttttc ccagtcggca gtcagacgtg
     4801 cagatttgat ttcatctggc atgacatgga tcagatttct gcccttgtca gtagaaatga
     4861 tcttcttgcc gtcacgctga atatatccgt gctttacaag attttcaatg gtgttggctc
     4921 tggtggcagg tgtgccaagt ccctttttct ccgcattttc gtcaaaatct tctgcacctg
     4981 cgtgttccat ggcagagaga agcgtatctt cggtaaatgc ttttggcgga gatgtaaagt
     5041 gttctgattt tactgccttt gccgtaaaag tcatgccctt ttccaaatca ggcagactct
     5101 tttcgtccga tttttccgct tctccaattt tgccgtctaa tgctttccag ccggattcca
     5161 aaacagtttt gcctgatgca gtaaatgctg tgttttcaca ttcgagtgtc acttttacag
     5221 catcatatcg atatgcagat gccgaagcac acaaaagcct taaagcaacc agtttcaaga
     5281 tattctgttc acctgttgca agtgccgaaa tatcacattt cacgccgctt tccgttggaa
     5341 taatcgcatg atgtcctgtg actttgtggt tattgatgat tgccctgaga ttcggttctg
     5401 caacacttcc aaacccaaaa tgttcgctga ttgcaggaat gagagcatag gcggtttgtt
     5461 ccatatcatc ggataagtac tgagaatcgg tacggggata ggtgaccagt ttcttctcat
     5521 agagggactg catatagtcg agtgtctgct gtgccgtgta accaaacttt ttgttggctt
     5581 cacgctgtaa ggaagtcaga tcaaaaagtt tcggagcaga tatgctcttg acctcctttt
     5641 tcacatctgt taccgttacc gttttgccat tgcaggaaga tgccagcata tccgctgtgt
     5701 tttcctcatc gattcttgca ctttctgccg ggaaacccat atccagaacg accgtgtaat
     5761 acttctgctt gacgaagttt gcaatatctg catcacgctt cacgatcata gcaagtgttg
     5821 gtgtctgtac tctgcctgta ttcaggtggg cgttgtatcg caccgaaaac agccttgaac
     5881 cattcatgcc gaccagccag tccgcctttg cacggcagaa gcctgcctga tagagattgt
     5941 catacacaga actgcttttc agcttataga aaccgtccct gattgccgat tcttccagtg
     6001 aggaaatcca aagtcttttc accggctttt gacactttac aagattgtat acatatcgaa
     6061 aaatacattc gccctcacgg tcagcatcgg ttgcacaaat aatctccgtg atgtttctgt
     6121 cattcataag ctgcttcaat actttgaact ggtccttaga attttcagaa atcttaaatt
     6181 tccattcttc cggaatcatg ggaagctgtg caaagctcca ttttgccgcc caattttcag
     6241 aatactcatc tggaaactgc aatccgacca gatgaccaaa acaccagctg acaacatagc
     6301 cgtttccctc taaccaaccg tttttcttgg tgtttgcacc gagaaccttt gcgagttctc
     6361 ggctgaccga aggcttttca ccgattacca gaatactcat acgaaatcat ctcccatctc
     6421 atcgctgtca cgatgtgcct tgttgtatcc cttttcataa cctttgcggt aaaaaattgt
     6481 tcctgcaatt acggcggctc ctgccgctgc aactgcaccg attattttgc aaatcctgtt
     6541 catgtaaatc caccctttca atatttaatt ggagtaccat aattgatctt tttgatactc
     6601 tgaatattgt caaaattcaa accataccag ttgtatgaac cgtaatttcc tgtgaaagaa
     6661 acgcagtccg gcaaatatcc gtcggcatgg ataaactcca cgatacactt gccgtcaccg
     6721 ccgaacgggt gataccaacg gtcgcccttg ctgtcacaga tctttacggt aaactgtgtc
     6781 ccgttttcca gcgtgatgag gaatcgatca ccgcagtagc cgaagaaagt tcccatggca
     6841 cagcagtaat caccatatgc atcacgcatg atgcctgtgt tggtaaccgg atcggggaca
     6901 gcaccctgaa tcgctgaata ggacggatct cttgtcagac cgcttgcttt ttcataggcg
     6961 aaacaatttg tccagccgtc tatgtagtca aattccccaa tcacttctac cgcttcggtt
     7021 ttctgcaccg cagatgcaag aatttctcca ttctcatcag aaagctcaaa tgtatattcc
     7081 gnnnnnnnnn nacgaagtcc tgtcacatag caaatggcat tggatttgaa ttcaaaatag
     7141 atgttgtcca catagtcatc attcacattt tggattgcgg taactgtgta ttccgtatac
     7201 gcatcgccgt tccattccag cttgatgcag gatacggaaa ttgtcgctgc cgtcagtgta
     7261 acagcgtctg tctgttcgtt ttctgcatag gctaatgttg ttgattctgt cgattttgta
     7321 cttgtggtag ttgatcttag agcggaatcc accggcattg cagatgtagt ctgtgttgtt
     7381 gcaggaacgg caatgtcctg tccctgcaca ttctgatcca gatcgtagtg accacttccc
     7441 agaaccacaa agaagcttgt gccgatagag atcagtttgc cggctagcat tgtactaatg
     7501 ggcatttcgt tcacctctgt tgatctgaat cactgccatt gtgaaaacgc cgatgcttcc
     7561 accgataata agacctaata ggaatttaag cattcgtatt ctcctttgct ttctttttca
     7621 tcattttctg aaacgctcgg tatttgtggg tatacgcata agaatctccg aatatattct
     7681 gtgctgcggt gtacagcttt ggttcatgct ttcggatgat ctccagttct tctgtcactc
     7741 tgggattgta tggacagccg acacagccgg tacgtttcat tccatattcc gtatagcacc
     7801 gggaatgctg tattccatag aactcctcat atgctttttt atcgctgtcc gtgtaccaga
     7861 atacaggacg ataagaattg cagcctttgg atttgctttc ggaaaaacac gttttgtagc
     7921 tggcagaacg aattccgccc tctgcctttc gtacaccgat aatttccagg tctgcatcgt
     7981 atttttgaat gagaagcttt gcaggctttt tcttggtgta ctcgcagcat ttgttagaga
     8041 ctttgaaatt cggcggattt gccatcataa actctttcag ccacctgttt cttccaatag
     8101 aaaagcggct cattttgacg attccatttt ccggggagta ataggcattg caccaccatt
     8161 gcagggcgat cttgcagttc gggtattgct ccagcaattc cggtaacggc ttgtcttccc
     8221 attggaatcc atgcagctgt aaccgcatga tctgttcgct gacgtatttg gagagaatgg
     8281 gctgaccata ttccttacaa cagactggaa tcggcttgca agccttgata cgttcgattt
     8341 cgataccata cttcttttcc agatcgttga gatgttcttt tgtagcacgg tattccagtc
     8401 cggtatcaat ccagaagtag cggactttct ttttctcgtc cacattgtgt atcacatcaa
     8461 gcacaatgtc gctgtcactg ccgccgctga tactgcaaag cggtgtgtgg gaacgggaga
     8521 gaacgctgac cgcttttgca taaccgtcaa taatggcaaa atttttggta tccagagaag
     8581 atgcaaacgc cttagacatt tttcaatcct cccaagatgt gtgcaatcac gtctactgtc
     8641 cagccattgc cgatacactt gtaccgctgt gtattgctga ttcctgcggt gtagttgtcg
     8701 ggtaacgtct gcaaacgttc cgcctccaca ggtgtcagct ttctaatgat gtagtcaccg
     8761 tccggcagat caattttgta aagtcctgtc tttgcaccca ttccgccgcc gtttgccgtc
     8821 attgtgatcg atttgccaca gacagaataa atccgctgtc cttgtccgcc gttgccaatc
     8881 tgaccaatgc gaatgggaac ggcgatcttc tgacggcttt tttctgcttt gtaaaccgga
     8941 aaaaggttag tcccagcctt gtagtaagtt gcaggaatgg tatgagattt gccgtttact
     9001 gtttccacag gaacagccac catagtacgc tgctttcgtt ccaaagtgtt atgaaaagca
     9061 gcaccctgat agctggctgt gacacagtag cttttttcct gccaggacac accgttttcc
     9121 agtatatcct gcaacagaat gcctttgtcc aaaggttgtg taacatgggg aatattcgtc
     9181 cagtaacaac gctttcggtg ctgtcctgac accaaggaag aattgatgac gattggctca
     9241 acaccaagtg ttctggaaat ctcatctttg atatttcggt gaatggaatg gttattctca
     9301 tagagaaagt aacggcattc cgattcctgt aaggctctca catattcatt gaaaagtttg
     9361 aaccctttgc cgttgcttgt ggtttctctg ccttgctttg cgatagacca gtatgtacag
     9421 ggtgaaccac ccaaaagcag atcatatccc ttgaattttc tgaaatcacc gtcaaacaca
     9481 tttccgtgat gaacaatctc agggtgattt ctgcttgaaa ccgttatggc atatttgtca
     9541 atttcaaaag catcataggc ttccacggga atgcctgcac gttccagtgc caccattcca
     9601 caggagatgc cgtcaaaaag gctcagtacc ctcatgcttc atcgtcctca tctgtcacac
     9661 tgccgtagtc atcgtcataa tcttcctcga aatcctcagc accagtattt ttcttcttgc
     9721 cgaatttgcc ctttttcagc acaaggacgg caccgccgat cacagccagt acaccgactc
     9781 caatgagaat gagtgcattg ctgttcatag atgaaggctg tttggtggta gatttttctt
     9841 ctgcacgctc tgtttcaccg gtggcttctt cctcacggtc agaaacatca gccgtcacac
     9901 cgccgttggt ggctgcatcc gcatatttct ttgcctgttc cgcaggatct ttttcttctt
     9961 ctccctcatt gcctgcatag agcagagaat acagatcgaa atcgtctacc ttgttcagaa
    10021 agaatacatt gtcctcatca ccctccgcag aataattgat caggatgtag aacacatcgc
    10081 cgtccttggt ggtgaccgca ataaactgca tctcctcact gtcgtagatg atcttctcct
    10141 gtttgatgag cgttgcatta ccgtccgtgt cgtaatggtc atcggcatag tagtcatgca
    10201 cagtgtcatc gatttctgca ccgctgtttt ctattgccgt tttgttctgc tggatctcgt
    10261 ccaccagtcg ctgcatttct ttctcaccga gttccatatt cgctgcggaa ttgtcagccg
    10321 ccgtaattgc agatgtggtc atatctgatt cctctgcaaa tgcaggaatg gcatttccca
    10381 cacttgcgag ggtaagcacc attgcagaaa atactgccgc cgcagatttt ttagtgttcc
    10441 acattttttc tttcctccat caatattttc tgttcatgca ccacaggaga agcagccgtc
    10501 agtctggcga tgatctcctc cgtggtcatg ccgttgtcat gcaaaaactt tttgagtgct
    10561 tccacttcaa gcatttctcg cttggctttc agttcctcaa gaagttgctt gtcctgattg
    10621 atcttttctt caagcttcgg aattttcttg ttgagagcat ccaaagcggt ttcttttctt
    10681 gtcattttca tgctccttag tagagtacca gtcttgggtc aatgtagtcc cagccgtaac
    10741 cgtccgtgtc aatctcaata tggaaattga cattcgtatt ggttgtttgt gcaaacattt
    10801 cgccctcatg gatcgtatca ccctccgaat agccattcag aagaacagca ttggcgatcg
    10861 tcacttctgt atctcgcttt gttccgccgg aaccgtcata ccaatactgc acatcatctt
    10921 ttcgcagggt gatcttgtga ttcgccgtgt ccacgtcctt gatcttgcag tcaaatggtg
    10981 catagagcct tgtacctgta ttgcagtaat acttgatgcc ctgatacaga ccattatttg
    11041 tccggttcca ttcccacata tcgtagccaa acgcctgttt gaccaaataa ttccgaatgg
    11101 tatcaccgga aagcatattg cgaagtgtct gatgatttcc atagagcgtt tccgaaccgc
    11161 tttcctgacc tgcaagaagc ttgtagtatt ccagccgttc ttccttatgg gattgacttt
    11221 tcagcttatc ttcgatcacc tgatcaaagc ttttcttctg cctgacgttg taatagagat
    11281 tgcactccgt tttccagtag tacttttcgt aatagccgta catacaggca tcatggatgg
    11341 tgtcattcca gatatgcgta tcggcgggcg aaaccagaaa cnnnnnnnnn ncattcccat
    11401 tccagcccca gaaagaacgg tcgtaccggg tgtcgccctt catgaagaaa aatttctgtg
    11461 catcgttgtc gatgaagtaa aacggttctt ttatgccgga ataatagcgg tgatccatga
    11521 tcatgaagcc ggtcagagca tagtcatcgt tcggattcag cacccggtag tccgcattga
    11581 tgcagacata gccctcactg tcaaagtact tgctcagttc agacgtatag gcaatgggct
    11641 tgaaacgata tttgtatggt ctgtcgctgt agacaaaggc atctttttcg gctctgtaat
    11701 acgtaccggt ttccgcactt ccgccgcccc agtagttgta gttggaaagt tcctcccagc
    11761 gggatttatt gtcataccag tagataaacg catattctgc attgaatatt tctgtcagca
    11821 gattctctgt gtcaccgcca tagctccaat acttgatgtc gccgttgctg tctgtatcaa
    11881 agtcatagta ataggcacac aggaacgacc acagcttgta cacgtcaaaa tcatagaccg
    11941 gatcccagtc ataaacggaa gaacgtcccc agtaccaatt gtcgggatcg tctttcagat
    12001 tacgcttgtt tgctccgaaa gcggcaagac cgtttttcca gtctgtatca ctgctgactt
    12061 tcagaatctt ctcattcatg tcgtaggcaa gttttgtgta atatttttct gcttccgaca
    12121 agtcgtagtc ctgagccgca tatgtgccaa ggacaaaacc cgaaccgctt aaaatgccgg
    12181 aaaagatcat gagaatgaat gccagcacca gagcgataat gagaatcggg acaaggcagg
    12241 cgagcagaac ggctcttgct tccttgttgt agatgttctt aagaaattga aaagcctgcc
    12301 gtagtgcatt tttcagtctg tccccaaagg aattcttggg ttgctttttc ggcttttttc
    12361 tgcgtttcac ctgttcgccc ttgcttttca gcttgtcctc acgcttttgc aatctccgat
    12421 gctgatctga tttcttctgt tccagtttcg ctttcttttt ctgattttgt tctttccggt
    12481 gatacggctt cattttttct atcgcttgat ctacaccgtg gcgtttcaca gattccaccg
    12541 cagagagaaa atcattgttt tcatcggcat gaactgcttt ctgccagccg cttgccgcaa
    12601 gtcgtttgcc tgtgtaatta gccggcttta agaccagcgg tgcaggtctg gacagatcag
    12661 cgatccgcca ttgctttcgg agtgtctttt tctgaaactt cttttctctt ttcagcttcc
    12721 gggataccgt tttgattctt ttagcatcgg ttttgtattg tttttttcgc ctttgaaatc
    12781 gatgtgcggc aacagctaca gtaaacgaat ccgatacttg tatcttcttg aaatgcttga
    12841 atgacttttt gcgttccctg aatttccgtt tttccgcttt cagttcctta cggtttttcg
    12901 caagctttgg acgatactgt tcgcccttga aaagcttgta ctctgccttt tgcaggcggt
    12961 aacgtgtctt ttccagtctg aactgctttt tctgcttgaa atgactgcgt gctccatgaa
    13021 ccgcatcttt tgcgatgaca agggttgcaa tcgtgccacg gtggtagtca tctaccgctt
    13081 cctgacggta cttttgcttt agtttgtatc gtacatttcg ttctgctgct ttggtggtct
    13141 tgagcgtaac ggtttctgcc ccaagagccg tactttctga ggtatgccat gccgtatgag
    13201 cagttcgatt gaccaactgt gccgagcgtt tcaggacttt tccacggaat gtttttggct
    13261 ttaaactatc aagagatttt gtgatggacg gtttatcgcc tgtaaacctc gttttgagat
    13321 tcactgtcct gtgaatcaga ccatgtccgg tatatcggct gcttttttct ccacgtcgga
    13381 tacgggcagt nnnnnnnnnn ttccgctttt gcaagctgat gatgtgtcct ttttagattc
    13441 gccatagcgt ttcgctcctg actatttcag ctgctgttcc aacggttttg tggtaagcag
    13501 actgtacatc acggtatcgg tcgggaaatt atccgtaaac ggcagaatga tcttatcgta
    13561 gcggatcaga ccttcaccgg gtccgctgtt ggtgacaaac tccatttgct gtggactgat
    13621 gtgcagctct tcggcaagaa tatcacggtc gccggcagcc tgattcagca tatatacaaa
    13681 gtccgagttg tcgaagatgt tttccacttc ggcactggac agcaagtcct tgacattttg
    13741 cgtaatgccg gtcggaacac cgccccattt acgaaatcgc ttccagattt cagcggaata
    13801 tttcgccgtc tgttcttcct ttaagagaag atgaaattca tcgatatagt agcgggtgac
    13861 ttttcgttcc gcacggttca gtgtcactct gttccagacg ttatcctgaa caatcagcat
    13921 accgaccttt ttcagctgat tgcccagttc cttaatgtca aagcagatga tacgattctt
    13981 caagtcaata ttcgttctgt gattgaacaa attctgcgag ccgttgacat acatttcaag
    14041 agagtttgct acacgaagtg cgatttctcc ctccttgaca agagcattgt gcagatcgga
    14101 cagaatcggc attttttctc tggacggttg attcctgaaa aaatccatgt agatggcacg
    14161 aacgcatcgg tcaatgacag aacgttcctc cgcagtcagt ccatacttgc cgcccacaat
    14221 gatctcacaa agagaaatga taaaatccga ctttgtggag atcggattct catccatgtt
    14281 gttttcaaag ttaatgtcca tgggattgat atactgttct gagctgcttg ctattcggat
    14341 cagctgtccg tgtaaatgct gcaccaacgg aaagtattcg ccttcagggt cgcagatgat
    14401 aatgtcatcg gttgttttca gaaagctgtc taagatttct ctttttacgc tgaatgattt
    14461 accggaacct ggtgtaccaa gtatcagacc atttggattt ttcagtctgc ttctgtttgc
    14521 catgatcatg ttgccggaaa cggtgttcaa accgtagtat gtaccgccaa tctgaaagag
    14581 ttcctgtgtg gtaaacggaa caaaaatcgc aattcccgat gtgtgcatct cacgggaaac
    14641 ctgtatctta ttgataccca gcggcagtga tgaggaaaga taatcctcct gcaagtagtc
    14701 acagggcatc agggtacagt tgttcttctg acagatacgc tttaacaatt ccagctgcaa
    14761 gcctaagtct ttcttatttt ttgcatagtt tcgtatcgta atggaaatat gaaacaagcg
    14821 ttcattcttg ctgttgaggt catccagcag cttttccaga tccgtaatgt acattttgat
    14881 tgccggagga agaatatccg gatcgtagcc tgctttggaa gcttttttct gttcgtcaat
    14941 tttcatagac tccacagcag tcagctttgt cttgacaaat ttgagtgccg tgatctgatc
    15001 cagcggtgta acgtgcagat tgacacagaa aaggtgctgt aattcgagaa aatcggaaag
    15061 aatcgtgtcc ggcagttctc ctgcaagaat gttcattcca ttgatcgcac cgaaagaagc
    15121 accaatctca aagtttgcct tattgaattt catgccggac ggagcaatga agnnnnnnnn
    15181 nngcaatgaa gtctttcgta ttccagcctg ctttcagcat ttcattccag tcaaagacaa
    15241 aaggggcact ctgaaatggg tttaggctgt aatagagtgt ttccagtctt tcttttccgc
    15301 tgagtgcctt tgcttccaca ttgaagccct tgaacatctt gataatttct gtctggatat
    15361 tcaggagttt tggctttgcc tgtttgtagg tacttgcttc aatggtgaaa gtcaggaatt
    15421 ttctgcatga ctgtccgttt ttgcccgaca acagctttga tgtcagcatt tcggagtatt
    15481 ctgcacgaat ctcattgaaa tcgtcctcct gtgcaggaat ctgaatttgc ttgaccagtc
    15541 cgtcctgact tctgttctga ttttcaaagg tgagctgaaa tgtgacggta ttgtcaaagt
    15601 aattgatcag atcgcagtac tttgagaaaa tgccgttttg ttcctcaaat tcagcaagct
    15661 gataattggt atcaaagaac tgaatggtct ttgaaaatct atggtcatcg atctggcata
    15721 cgccgtccct gaacatctgc gtatagccga tcgtattctg tgtggacgaa atatcgtcct
    15781 tatatttctt gatctcgtgc attcgtgcca gaatgactgc cttatcattt ttgtcaatgt
    15841 tagaaattgg cttttcagcg gcagcttttg ctcccgacct gagatctgtt ttcttcttgt
    15901 ttttcaagcc tacttgctcc tttctatctt gtccaaaagc tttttcagct tggtatattc
    15961 tatctgtctt tcgatacatt cgtatagatt gaccgttctg taagttcgct ttctcggtct
    16021 tttgaaatag cggaacatat tcttgatggt ctgctcgaaa aagatcccgt ttttcttata
    16081 caatccgcag atgagagcag gtgctgccac tgcacccatg acaaagattg ctcctgaaac
    16141 gccaagagta ctgcgtgtca gaaagaaaag cggaattccc agcacaccac cgattccaaa
    16201 cgaaaccacc tgtcttttgg tcagtccgaa catgaacttc tgtttgatgt cattcaagtc
    16261 ttttggtatt tgtacataat gtgccattca caacctcctg aaaatcaatg tgcattgaaa
    16321 atagattttg aaacagcacc tgtacggagt accgtaaaga tcagtgcaac cgtgtatccc
    16381 agaagcattg ccatttgcag aataatgccg tcagtactgc tgttcagcga tgcaatggca
    16441 ttggagaaca gcgtcttgaa gataccaagt gcaatgatga taaagaatcc ctgaaacgat
    16501 aatgccagaa gctgtttgat ccagttcttt ccgattcccg accattcacc gctatccagc
    16561 atagctgcca tgggaatcgg tgcaacgcct aagtacataa agacctcaat gatacgggaa
    16621 gcaagcacaa ttacaatggc aaccatcata atcattacac ccaagtgaac aatcagcgag
    16681 ataaaccaga cggttatcag ttcaccaagt cccagactgt tcagagccga ttttttgagt
    16741 gccaagtcag ttgacaggaa atctcctgta ccgaacagcg ttgtaatacc gttggcacaa
    16801 gcgtctgttc cgaacgaaaa cagggcagat gcgatgtaaa atacgtttga taccaaaatg
    16861 acaccgcata aggctttgat aatccatttg ataaagatgg aatcatcaaa atccttgaag
    16921 ttatttccac gaattacggt ttgtatcaga tcattgagca atatgatgac aagaatgaaa
    16981 ccgccgatgg gaactaccac gttattgcaa agtgtttcaa tcgttgacca tatggttgtt
    17041 ccgccgcttg cagaaccgga aaaacttgca ggatgtcctg tcagaaatgt ggaaatcaga
    17101 ccgccgtctt ccgtggtttt gtgaaaggtt tctgtcatca ggtcggatat gctgtcaaac
    17161 tgcgatttga taccgtcctt gaacagattc tcacaccatt cctgtaagct gtcgatcgca
    17221 tcacttatga cgcccattct ctcaccgtcc tttccttatg ccggctgccc acacaccacc
    17281 cgccacgcag ccgttaaaaa attcggtcag attaaacggc accggtcatc atgctctgaa
    17341 gaaccggaac gaggacgatt gcaaggagaa tcagtccaag accagccata agctgcttca
    17401 tgccctgcga tttcgcaccg gggttatcgt taccgtaacc ttcaaggagg ttgacaacac
    17461 cccagacacc gacacctgca ccgatcaggc agatcacgga cttgagtaca gtgcaagccg
    17521 tactgatgaa agaagaagta tccacttcac cggtagcggc aaaagcctgc gtggtacaca
    17581 ttgctgccat agaggacatt accgttgcag tgagaattgc tttctttgcg aagcggctgc
    17641 cctttgcaag ccacttatgc tttggctttg tgttttcaac cgcagtatca gaaactgctg
    17701 tcatgacatt tacattttcc atcgtaattt ctccattctg tataagtatt tatctgttgc
    17761 tgaaaacagg aatcaaaaag ctctgtcgat gttgttggtt ttcttcggta ttcgctgctt
    17821 tcaggcaaga tcttcgtttc cgtccgtttt gtacacatct acctttgtgg tgcttttcag
    17881 tgtcagataa cgtttttccc gtttcctgac aaaggctgca atatcaaatt tcgctttcgg
    17941 attatcgtcc agcaaaaggt gatagtgctt atgttttgta atatcaaact tatctgagaa
    18001 gaacggacgg acacctctca gctgtaagat acatttgccg ccgtccatga cggctaattc
    18061 gtcctgcgac tttaattcct tgccaagttt ctgataattc agcccatagc tttcagactg
    18121 acctctcgtg ttggaggtgt taaaactgtc tatcgtttct tttccaagcg attccgagat
    18181 ttccttgaga gtggatttct ccttgcctcc aagaaacagg gtggtatcgc agttgccctc
    18241 aatggtatcc gcattgtctt tataaattgc ctttagctga gattttgcct gcaagatgat
    18301 actggcagag atctcacggc ttcggatcgt ggcaatcagc ttttcaaact gcggtatttc
    18361 gccgatattc gcaaattcat cgagcaagca gcgaacatgg tagttcagct tgccgccctc
    18421 tgaattatcc gcttttgtgc ataaaagatt gaacaactgc gaatacatga tcgcaaccag
    18481 aaaattgaat gttgcatcgg tatccgagat gataacaaag agtgcagata gttcatcgcc
    18541 cagcttgtcc agaccaagtt catcatagct tgtgatttct aagacttcat caatggcaaa
    18601 aggggctaag cgggtagaac aggaaatgag gatcgacttt gcagtctttc cagagacgaa
    18661 aagtcaagga ctttttcatc aaattcaaaa agaagtcaca ttt
//
LOCUS       DAWBGR010000028        18299 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1033_length_18299_cov_4.758222, whole genome shotgun sequence.
ACCESSION   DAWBGR010000028 DAWBGR010000000
VERSION     DAWBGR010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 18299)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18299)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18299
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1033_length_18299_cov_4.758222"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7209..7218
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4363 a         4630 c         5508 g         3788 t
ORIGIN      
        1 gatcgtctgg cggaaatgac cgatgccgtg gaaaagctgg tgccggaaac cgtgcagcag
       61 tactacgaaa agctgtaccg gaaaattacg gaattgctgg gcgaccgaac tgtggacgaa
      121 tcccgtctgg tgacagaagc tgccgtggtt gcggaaaaaa tcgcggtaga cgaagaactg
      181 gtgcggctgc ggagccacat tgcacagttc cgtgtattcc tgaccagtac ggaaccggta
      241 ggcagaaaaa tggattttct ggtgcaggaa ctgaatcggg aagtcaacac caccggctca
      301 aaatcgcagc atttggaaat caccaaattg gtggttgata tgaagtctga aatcgaaaaa
      361 attcgggaac agattcaaaa tatcgaatga gaaaggaagt atgcagcatg aaaaaaggaa
      421 cattgtttgt catctccggt ccgtcgggct gcggaaaagg caccgtgctg aaagaggtgc
      481 tgaaaaatcc tgatctgtat ttttccgttt cggcaaccac gcgtgatccc cgccccggcg
      541 aagtggacgg tgtcaactat cgctttatga ccaacgaagc ctttgaagcc ctgatcgaac
      601 aggacggttt cctggaatat gccggctact gtgaccacta ttacggttcg ccgaaacagc
      661 cggtggaaga acagctggca atgggaaaaa gtgtcattct ggaaatcgaa gtcgaaggtg
      721 ctatgaaagt ccgtgcggct cgtccggacg cagtgttcgt gtttatcctg ccgccgtcta
      781 tgacagaact ggaaaaccgg cttcgcggtc gtggcactga ggcagaagaa gtggtgcaga
      841 agcgtgtggc acaggcaaaa cgggaaatcg gttttgcgga aaaatacgat tacgttgtga
      901 ttaacgatga cttgtctgcg gcgattgctg atgtcaatgc aatcctgtgt gcagaatctc
      961 ataaaacaaa gaaaaatctg gaagaaatca aagaggtgct tggaaaatga atatgttaag
     1021 accgtctgtc aatcagctga ttacaaaaaa tgaaagcagt tattctctgg taatcggcgt
     1081 ggcgaaacgt gcccgcgaga ttgccgatga actgtatgaa aatggaaaaa cattagagga
     1141 aaagccggtg aaaacagcca ttcgcgaact ggctgccgga aaatatcgta tcgtagacaa
     1201 cttcgaagaa ccggctcaga accactaagt aaatggaaaa acaagcggaa ttgccgaaaa
     1261 tctggtgtgt aggcgtggca gtctgtgcgg cttcttatac gtttgaccgg ctgttttcct
     1321 acattgccat gcaggagatc cctgccggct gccgtgtagt cgtgccgttc ggcaagggaa
     1381 atgccaaacg tgtgggaatc gtgctgtcct gtgcagaaca ggctgcccag cccgaacagc
     1441 agctgaagcc ggtgcttgca gtggtggaca aacagccgat cctgtcgccg gaaatgctgg
     1501 atctggtgtt ctggctcaag gaaatgacgt tctgtacata ttacgatgca gtgcgaacca
     1561 ttttgcctgc cggcatgcag gtgcagctgg tggaaaccat ttcgctggca gagccgcagc
     1621 cggactgccc gctgacagat gccgaagcac acctgctgtt tttcctgcgg ggagcaaaga
     1681 ccaagcagga attccggcgg attctggcgg acgcagatac acagggcaaa aaaatcgtgg
     1741 atacgctggt acagaaaggc tttctgctcc ggcaggaaac ggtaaaggaa aagacccacg
     1801 gacagaccgg catcaagatg ctgcgaatgg cagccccgcc ggacgaagtg ccgcctgtga
     1861 ccaaaaccca gcagaagctg gtgcggatct tacaggaaac cggtgccgtt tcggaaaaag
     1921 aggcgtgcta tctcagcggc gtgaccacgg cagtggcgaa aaagctggtg cagaacggcg
     1981 tggtggaatc ctatacggtg aagccgccgc tgcacacata tgccgtcagc ggtcagaccc
     2041 gccagcccgc agacatcgtg ctgtccgcgg aacagcaggc agtatatgac cgtatggttg
     2101 ctgcactggg cgagggcatg cactgctttt tactgcacgg cgttaccggc agcggcaaga
     2161 ccagcgtgtt tatccggctg atcgacaccg tggtgcagcg tggaaagcag gtgatcctgc
     2221 tggtgccgga aatcgccctc acgccccaga tcgtggaaca gttctatcag ctgttcggca
     2281 atatcgtggc ggtgatccac agtgagctgt ccttggggca gcggagcgaa acgtggcgga
     2341 gcatcgcttc cggtgcggtg aagatcatca tcggcaccag aagtgccgtt tttgcaccgg
     2401 tgcagaatct ggggctgatc gtcgtggacg aggagggcga gcgtacctat aagtcggaca
     2461 gtgccccgcg gtatcatgcg atctccgtgg cgaaaaaacg ctgccagacc catcactgcc
     2521 cgctgctgct ggcttcggca accccttcgg tggaaagcta ctactttgcc aaacggggaa
     2581 tctataccct gctggagatg caccagcggt ataatcagac accgctgccc cacgtggaag
     2641 tggtggatat gcaggaggag agaaccctcg gcaaagaggg catgttctcc gaaacgctgg
     2701 cacgggcact gcgggacaac ttagaatcgg gcagccagtc gctgctgctg ctgaaccgcc
     2761 gcgggtatca taccattatc agttgtgcgt cctgcaatca gccggtgtac tgccccaatt
     2821 gcagtatccc catgacctat cacaaagtca acaacagcct gatgtgccac tactgcggac
     2881 atacgcagga catggtggaa acctgtccct cctgcggcgg aaagcacctg cggaaaatgg
     2941 gctttggcac ccagcggctg gaagaagaac tccggctgat cgtgccggaa gcacggatac
     3001 tccgcatgga cgcggacacc accatgtccc gctatgccta cgaggaacgg tttcaggaat
     3061 tccggcaggg caagtatgac atcatgctgg gaacgcagat gatcggcaag gggctggatt
     3121 tccccaatgt cacgctggta ggtgtgctgt cggtggacaa ggctctgtat gccggcgatt
     3181 tccgcagcta tgagcgaacc ttttccctga ttacacaggt cgtgggacgg ggcggcagag
     3241 gtgaaaaagc cggccgtgcc attttgcaga ccagtatgcc ggagcattat gtgctgcggc
     3301 tggcggcaca gcaggcgtat ccggcgtttt acgaacagga gatcgccctg cggcggcagt
     3361 tgatcttccc gccgatctgt gatatttgtg tggtgggctt taccggcatg cgggaaccgg
     3421 acgtgcggac agcggcaatg cacaccgcag acgtgctcaa agatcagatc cggaaaaccg
     3481 gcttttccta tccgatctgg gtgctggaac cggtgccctg tgtttacggc aggatcaacg
     3541 gaaaataccg gtatcagctg gtgatcaaat gtaagaatac caagccctat cgtgatctga
     3601 ttcatgcggt gctgaccgaa gccggtgcag accgcagctt tgcccgcgtg catctgtatg
     3661 cggacatgaa cggcgatgtg ggcatatgag actgtcctga agttgcgggg caataggagg
     3721 aatatctatg gcaattcgac gtatcgtaaa agagggcgac ccgattttac gcatgaagtg
     3781 ccgtccggtg accgagttcg gagaaaagct gaaccagctg ctggacgata tgcacgaaac
     3841 attggaacag gcgaacggag ttggtctggc tgcaccgcag gtgggctact gccgccgttt
     3901 tttcattatg catctgggtg atgtgaaaat cgaagcgatc aatccggaga tcaaaaaaac
     3961 cagcggcaaa cagtatgtgc tggagggctg cctttccgtg cccaacaagt ggggctatgt
     4021 gacacgcccg aacaagtgca agttccgtgc ccagaaccgc aagggtgagt ggtacgaaat
     4081 gacactgtcc ggactgggag cacagtgcgt ctgccatgaa aacgctcatc tggacggaca
     4141 actgttcatt gatgtggtag aagaatttgt agatccggag gatttggaag aagcatgaga
     4201 atcgtttata tgggaacgcc ggacatcgca gtgccgacgc tgcggtttct ggcagagagc
     4261 aagcatgaag tcgtaggcgt tctgacccag ccggatcgcc ccaaggggcg gggacaccac
     4321 accattccca cgccggtgaa agcggctgct ctggaatatg acattccggt ataccagccc
     4381 ctgtccctgc gaaaagggga agatgcagcg gcggctctgg aaacactgcg gcagctggca
     4441 ccggatctga tcgtggtgct ggcatacgga cagcttttgc cgaaagaggt actggaactg
     4501 ccgaagtatc actgcatcaa cctccacgct tctctgctgc cggcataccg cggagcagcc
     4561 ccgatccagt gggtgattct gaacggcgaa accaaaactg gtgtcactgc ccagcaaatg
     4621 gcagagggac tggacaccgg tgatatgctg gtaaaaaagg agatcgccat tggagcagag
     4681 gaaaccgcag aggaactgta tgaacggctg tcggaactct gcgtggaaac cctgagcgaa
     4741 acgctggaac agctggaagc cggcaccttg cagggcactc cgcaggacga tgcacagtcc
     4801 tcttatgcat cgcgtatcac aaaggaaatg tctgcactgg acttcacccg tccggcggca
     4861 gaactgcata acctgattcg cggcatcacc ggatttacca tgctggaggg caaacgcctg
     4921 aaggtgtatg gttcccgtgt ggtagagggc gtttccggag aagccggaac gattgcagag
     4981 ccggaagatt tcgtcgttgc atgcggtgac ggaaacgggc tacggttcac ggagattcag
     5041 ccggagggca aacgccgcat ggcagcggca gattttctcc gcggcaagaa gctgaaaaaa
     5101 ggtgaaaaac tggggaacgc agaataagat gtgtaaacga aagtgaggga agcctatgca
     5161 gttttacgat ggttggtttg atgtgggata tggactggtg atcgtcggcc tgattatcag
     5221 cctttgggca tcgtggaatg tgaagtccac attccggcgg tatcagaatg accgcaacag
     5281 ccgcgggctg acggcggcac aggtggcacg gcagattctg gacgacaacg gtctgtatca
     5341 gattcgcatt gaacagatcg ccggcaatct gactgaccac tatgacccca gcggcaacgt
     5401 gatccggctg tccgagagtg tgtacaattc cacctctgtg gcagctatcg gcgtagccgc
     5461 ccatgaatgc gggcatgccg tacagcatga ggtgaactac gtccccatgc aggtacgcag
     5521 tgccattatt cccattaccc agatcggttc ccgcttctgg tatatcattt ttgtgctggg
     5581 catggtattc agcaattctt atatcggtgc cccgctgcaa atgatcggca ttctgctgtt
     5641 caccctgatc gtggtgttcc agctggtgac actgccggtg gaattcaacg ccagccgccg
     5701 tgccatgcag acgctggaga gccggtatat tctctccggt gaagaactga ccaaggcaag
     5761 aaaagtgctg accgcggcag ccctcaccta cgtggcagcc ctgctcaacg gcatcttaca
     5821 gctgctgcgt ctgctgtcaa tttcaaagag gaacaatcga agatgagtgc aagaaaactg
     5881 gcagtacagc tgctgaaccg aaccgaacag gaacagagtt attccaattt gctgctggac
     5941 agtgccctgt cccagtccca gctggacgaa cgggacaagc ggctctgtgc ctctctgtac
     6001 tacggcgtga tggaacggcg tattacatta gatgcggtgc tttcgcacta cagcaagcag
     6061 cccctgcgga agctggacag caccgtgcgg aacattctcc gtctgggcat ttaccagctt
     6121 ctctactgcg accagatccc cagccgtgca gcagtcagtg aaagcgtcaa gctgaccaga
     6181 agctgccgga aagccagtgc gtccggcttt gtcaatgcgg tgctccgcgg tttcctgcgg
     6241 gacgactgca agattccgta cccgaaagat cccagggcgg caatggctgt ggaatatgcc
     6301 gttccccagt ggctgctgga aaagctggtg gacgcttatg gtatggatac cacaaaggca
     6361 tttctggcgg acgctctcca gccggctccg cggtttatcc ggcagaatcc ggcggcatgc
     6421 acctgtgcag aactggaaca ggcattggga gatgccattc gtcccacaaa tctgacaggt
     6481 gcctatgaac tgcacgccgg cgatattcgc aaactgccgg aattccgcaa gggctggttc
     6541 tatgtgcagg atctctcctc ccagatctgt gctctggcac tgggggcagc gtccggcgaa
     6601 acagtactgg acttgtgtgc tgcaccgggc ggaaaaacct gtaccattgc ggcaggcatg
     6661 gagcagaccg gctgcgtaca tgcctttgat ctggcggagc atcgggtgaa gctgatacag
     6721 gacaacgtga agcggctggg gctgcggaac gtgcaggcgg cacagggcga tgccacccgg
     6781 tttcaggaag cttacgccgg tgcagactgt gtgctctgtg atgtgccctg ctccggtatc
     6841 ggtgtgctgc ggcacaagcc ggaggtcaag gaaaaatcac cggaggcact ggcagaactg
     6901 ccggcactac agctggcaat tctggaaaac ggttcccgct atgtgaaagc cggcggaatc
     6961 cttcagtact ccacctgcac cattctcaaa gaggaaaacg agggcgtggc agaagccttt
     7021 ttgcaggcac acccagagtt tgaaccggta ccggtgtatc cggctctggg cggcgtactg
     7081 gaacagccta tggcaaccat tctgccggag atgttcggca gcgacggctt ttttgtggcg
     7141 aagttccgga aaaaaggata gggcaatacc gcacaaagag cgtctggcga ctttgcacgc
     7201 catctgctnn nnnnnnnnca atctttgtgc ggtattgcca taggatcact gcattctgaa
     7261 aggaggaagc caatggaaaa aatcgacatt ctgtccctga caccggaagt gctggggcag
     7321 catctggcgg agatggggga accgaaattc cgtgccaaac agcttttttc atggcttcac
     7381 cagaagcagg tcacggaatt cgaccaaatg actacacttt ctctccaact ccggcagaag
     7441 ctgaacgaaa aattttgtat aaatagacta aatattgcaa gaaggcttgc atcttctacc
     7501 gatgatacgg taaaatactt gtatgagctg ccggacggca actttgtgga aacggttctc
     7561 atggattatc accacggaaa aagcctttgt atttccacac aggtgggctg ccgtatgggc
     7621 tgccagttct gtgcatcgac cattgccggc tatgtgcggg atctgatgcc gtcggaactg
     7681 ctgctgcaaa tttacgaaac ccagcgggat gccggctgcc gcattgacag cattgtgctg
     7741 atgggaatcg gcgagccgct ggacaacttt gaaaatgtgg tgcagttttt ccgcattctc
     7801 tcacaccccg acggcatgca gatgagcctg cggcacgtgg cactttctac ctgcggactg
     7861 gtgccgcgga tccggcagct ggcagacctg cggctgggag tgactctttc ggtatctctc
     7921 cacagccccg agaaccggca ccgcagtgag atcatgccca taaaccggcg gtatccggtg
     7981 gaggaactgc tggacgcatg ccgctactac ttcgacacca ccggcagacg ggtgaccatt
     8041 gaatatgcag tcatcaacgg tgtgaataat accagggcag atgccctgca gcttgccagg
     8101 cggctccgcg gcatgcagtg ccatgtgaat ctgattccgg tgaatccgat cagggaacgt
     8161 tcattccggt cgttccggaa agatgtggaa cggttccaga gagaactgac cggactgggc
     8221 atcaacgcta cgatccggcg gacactgggc agcgacatac aggcggcatg cggacagctg
     8281 cggcgtgacg cagaccaaca gaaacaaacc gccgcacaaa ctgtgaaaaa tgaatccggc
     8341 aacacgctct ttggaggtgg taaaaattga cgagtgcatc gcaaacacat attggctata
     8401 ttcgagatga aaatcaggac cgcgtgggaa cgcggcagtt cggagagaat ctgctgctgg
     8461 tcgtctgtga cggcatgggc ggcgaacgct ccggcagcaa ggcaagttct atggcgatag
     8521 acgtcttttt tgaacatttt gaagaaggct acaccgagga catggacgca tacagcgtgc
     8581 gttcgcttct gctttcctcc gtgtctgcgg cgaattccgt agtatatacg accgcccgca
     8641 tggactacca gaactttggc atggggacga cctgtgtagc cgcttttgtg acagcggaat
     8701 acatcgccgt ggcgaacgtc ggcgacagtc gggcgtatct gctgaccagg gaaaccatgg
     8761 agcaggtgac cactgaccac actgtggtgc atatgctcat ggaaaccgga cagatcacag
     8821 aagaagaaat ggaaacccac cccaagcggc atatgctgat gaaagcggtg ggggtagaac
     8881 ggacagtgtg tccggatttc ttccgcattg atctgaagga ggggcagacc tatcggctgc
     8941 tgctctgttc tgacggtctt tccggattct gctccgatgc agaaatacag gaggtcttgc
     9001 aggaagatgt ttccgatacc gaaactgctc agaagctgat cgacgctgca ttggaaaagg
     9061 gtggacgaga taatatttca gttgccattg caactgtgtc acataatacg gaggaatgag
     9121 aagcatggat aaggataaac tgataggaac acggctggac ggtcgctatg aactgacaga
     9181 tctggtcggc gaaggcggca tggcgaatgt ttatcgtgca tcagatgtgc tggataatcg
     9241 tgttgttgcc gtgaagattc tcaaaaatga atactccgag agtgaagaat tccagcggcg
     9301 gttccgcgac gagtccaagg caatcgccat gatgtcccac ccgaatattg taaaaattta
     9361 cgatatgggc ttttcggaca agatgcagta tatcgtcatg gagtacattg acggcattac
     9421 gctgaaagac tacatcgaca gcgaacacgt gctcaactgg aaagatgcgg tgcactttgt
     9481 cgtgcagatc ctgcgggcac tgcagcacgc ccacaaccgc ggtatcgtac accgtgacat
     9541 caagccgcag aacatcatgt tgctgacaga cggcaccatc aaggtcatgg actttggtat
     9601 tgccaagttt gcccgcgagg aaagccgtac tgcgaccgat caggcgatcg gcacggtgca
     9661 ctatatcagt ccggaacagg cacggggtga cgtgaccgat gccaagagcg acctgtattc
     9721 cgtgggtgtc atgttctacg agatgctcac cggcagaaag ccctttgaca cggacaatcc
     9781 ggtttccatt gccgtgatgc atatgcagaa tgtggcagtg cgtccccgtg acatcaaccc
     9841 gaatattccc tccggtctgg aggagatcat catgcacgcc atggagaaag acgcttccaa
     9901 gcggtaccag acggcggcag aaatgattcg ggacattgaa gcgttcaagt cgaacaatca
     9961 gatcattttc ggctattata acgctccgga gccgacccag tatttcaccc cgccggaaac
    10021 cggaaacaga cgggtacccc agcgggaacg ggtggcttat gagcagaacg gcggcggtgg
    10081 ccgcggcgat tactacgagg actatccgcc ggaaaacgag tcggaaccgg agcagtccaa
    10141 gtcgctggtc attccgattc tgacggcagt caccattgtt gtgctggtcg ttgccgcaat
    10201 cgtgattttc ctgatcgtgg gacagggtac caacggttcc aacagctcca ccgatgaagt
    10261 gccgaaggtg atcggcatga actacgatga tgcagtgctg aactacccca acatcgattt
    10321 tgaagtggcg gagcaggact acaccgacaa gtatgaggaa aacatcatct ataagcagag
    10381 catcgaagcc ggacagttcg tcaagaagaa caaggacggc aagatcgaac tggcagttgc
    10441 cgtcagcaag ggtgtgcaga aggtacagat ccctgacctg accaactata cagcacagga
    10501 agcagaagat gccctcaagg cactgggact gactgcggag cagaagaaaa cccagagcac
    10561 ctccgaggag aacatcgaag ccgaccacgt tattcgtacc gatccggcaa tgaatgagga
    10621 agttgccgtt ggctccaacg tagtgatcta cgtcagtcag ggtgttgccg tagacgaaac
    10681 cgacgtgccg aagtttgtgg gcatgacaca ggaagatgcc cagaaagagg cggacagccg
    10741 caagctgaag ctggagatca ccgaagtgcc ctctacagaa gaaaagggta tcgtcaccga
    10801 gcaggatccg gaaccgggca aggtcgtggt ttccggttcc accattaagc tgaatgtcag
    10861 caacggcgaa cagccggagg gtgaagtcac ctatggtctg gagaacctgc cggtttccaa
    10921 catgagcggc atgtacacct tgagcttcac caccgatgag gacggtgtca ttgcctcccg
    10981 ccagttcgtt gccgagagcc tgaccaatgc ttctgtcagc gtgaaagtca agggcaaggg
    11041 cagcaagatg gtaaaggttt cgatcaccag tgcacaaagc ggcaagaccg aggtgctggg
    11101 aacgtacacg tttgactttg ccaataagaa gttcactgcg tccagcgaga acatcgaggg
    11161 tgctctcagc ggagttgcag tgcagactgt tccggcagtg actgcggcac cggcagaaac
    11221 tgccgcaccg gacggtgcac catcggaggt ggaatgatct gatgaaaaat ctccctgttg
    11281 ccattcccga aaccgcacag ggcagaatta tcacatcggt aggaggtctc tatacagtag
    11341 agaccttttc cggtatctgc acctgtaaag caagaggcat ctttcgaaaa cgcggcatca
    11401 cgccctatgt gggggactat gtgcagctgt ccgaggacgg cgtgatccag gagatcctgc
    11461 cgcggaaaaa cagcatttta agaccgccgg tgtccaatct ggatcagatc ttctttgtgg
    11521 tgtctacctg tcaccctgct ccgaatctgc tgctgctgga ttcgttcctg acggtcgcca
    11581 tttatcagga tattcagccg gcagtggtgc tcaccaaaat ggacctgcac agcggagaac
    11641 agctggaaaa gctgtacacc gatgccggca ttccggtgta tccggtggaa tacgacaagc
    11701 cggaaacggt cgccgccgtg tcagaactcc ttgccggaaa aatcagcatg atgaccggca
    11761 attccggcgt ggggaagtcc acgctgctca acgccattga cccgtccctg caaatcgcca
    11821 ccggagagat cagcgagaag ctgggacgcg gcaagcacac cacccgacag gtacaactgt
    11881 atccctgtgc cggcggctat ctggcggaca cgcccggatt ttccaccttt gaggtggagc
    11941 agtatctgca aatggacaag gaacgcattg cagactgctt tccggagttc cagccctatc
    12001 tgggacagtg ccggttcagc gactgtgcac acgtctgtga aaagggctgt gccgtggtag
    12061 aagctgtgca ggcggggaaa atttccaaga gccgtcatga tgcctacgtt tctctgtatc
    12121 agacggcaaa gcagcgaaag gcgtgggaat catgaagcag gcagtgattt ttgcaggagc
    12181 accggtacag ccggagttac agccgccggt gccgcaggca gacttgtatc tctgtgccga
    12241 tgccggcgtg cggctggcac aggctctggg aatcgttccc gatcgcatca tgggtgattt
    12301 cgattcgctg ggcaatgttc ccgccgggga aaatgtggaa gcgttttcgc cggaaaagga
    12361 cgacacggat attttgctgg cggcaaaata tgccttgtct gcggggtgca gccgtctgat
    12421 tttttacggt gcactgggcg gcagactgga tcacatggtg gcaaatcttc agatgctgcg
    12481 gtttctggca gaccggaacg cacagggcat actggtagac catgcccatt ggatcacgct
    12541 gcagcgtggc ggaacggcat cgtatcccag acgggagggc atgtactttt ctctgtttgc
    12601 catgacggaa cggtgcgaag atgtcacgct ggagggtgtg gcgtacccgc tgtgtcacgg
    12661 ggtattttcc aacgcgtttc cgctgggtgt cagcaacgag atcctcgcaa aaacggcaaa
    12721 ggtgactgtg ggcaagggcg atttgctggt gatgtatgcg aaagactgat gcggcaaatc
    12781 agatttgtgg cagtgtctgg gaaaacctgc tgggagaaaa ctttctgagg aaagttttcc
    12841 cccagacccc ttttcaaaga cttttattcc atttttgtgg ataggggcta tagcccctat
    12901 ccacaaaaat aaagtgaagt tttcggaagg gagtttgagg gagaaccctt tttcaaaagg
    12961 gtttccctca acaagctttc ccagacatcg ccataaaatc tgatttggca ggaggaacga
    13021 atgagcgaaa aacgagaact aaaagaaacg cttcttttcg agaagaatca tcgaatccac
    13081 ggtgggatct atcataaaat gcaggtggat tttgcctata attccaatca tatagagggc
    13141 agcaggctga ctcatgaaca gacctgttat attttcgaaa cccaaagcgt ggacggtgct
    13201 gtgttcgtca acgatgtttt tgaaacggcg aatcatttca ggtgtctgga ctgggtgctg
    13261 gacaccattt ccgaaccgct gtcggaggaa tacgtgaagc agatgcaccg cattctgaaa
    13321 gccggactga tgacacaaca gaatccggaa gtcgtgatcg gtgattataa gaaatacgcc
    13381 aatgtggtgg gcggcatcga aacggcaaag cccggtgagg tatccgcaca aatggcacag
    13441 ctgctgcaaa cttatcagaa tttacagcag ccggacttct atgagattgc gtggtttcat
    13501 atggcgttcg aaaagataca cccattttat gacggtaacg gcagagtcgg cagacttctg
    13561 ctgctgaaaa tgtgtctgga aaacgacatt gtgccgttct atatcaacag cgaaagcaag
    13621 atgttctatt atgccggtct gaaagaatgg cagacacagg gcaagcccaa ccgtttgctg
    13681 gatgtgttcc tgtctatgca ggacgacatg aaagctgtgc tggactattt cgaaatcgac
    13741 tacgatcaca ccgaaaccac tgccagtaca ctggcagagc aacacaaaaa gtggaagctg
    13801 taacagtatt gggacagcct gttgagggaa acccttttga aaaagggttc tccctcaaat
    13861 tcccttccga aaacttcact ttatttttgt agataggggc tatagcccct atctacaaaa
    13921 atggaataaa agtctttgaa aaggggtctg gggaaaaact ttcctcagaa agttttcccc
    13981 cagcaaactt cccgaaacac agccggaagc ttgaataatt ttatgacctc cgtgtacctg
    14041 aattggtgca cggaggtttt ttgcatactc gtccgatgcc gtgcatagaa tgggacaaac
    14101 acagggacag cccatgaccg acaggaggac gtatggaaca gaatggaata caggctgtgc
    14161 tgcactgtct gccgccgcag atccgcagcc ggatacagca gctttcggcg gcagaactgg
    14221 cacaggtaca ggagattcgg ctgcgggcag aacgccccgt ctgcatacag attcacggca
    14281 gggaacagct attgcagccg gagagtgtcc tctcccagaa agccgtggaa caggtgtttc
    14341 aggcggtgtg tgaatactcc gtctaccgct atgcaaagga gatcgcagag ggcttcgtca
    14401 ccattgccgg cggcaatcgg gtgggcattg ccggctcggc agtctatcag gaaggcaaac
    14461 tggtgcagat gcggcatatc agcggactga atttccgcgt ttcccacgcc gtgaccggct
    14521 gtgccgaatc gctgtaccgc cggacttgtg ccaggcagcc tgccagcctg ctgctggtcg
    14581 gggcagtcgg ctccggaaag accaccatgc tgcgggatct gtgccgactt ctgggggctt
    14641 ctcaccggct gacactggtg gacgaacgca gcgaaattgc cgctgtacac cacggtgtgc
    14701 cccagtatga tgtgggactg cacaccgatg tgctggacgg ttttccccgt gcagagggac
    14761 ttctgacggc actgcgggtg atgacaccgg aggtgctggt ctgtgacgag atcggcacag
    14821 aggcagatgc acaagccatt ctggggatac atgggtgcgg cgtgccggtg atcgcctctg
    14881 cccacgggac atcatgggag gacttggaac gccgtccggt gctgcggcag ctgctggaac
    14941 agggcgtgtt ccgctatgcg gcgattctgt ccgtgcagga acagggcgtt gtccggacgc
    15001 tgcggcaggt gacagcatga tgaagtggct gggactgctg ctggtagttt gcagcggcgg
    15061 cggtatcgga ttcctgtatg ccatgcggct gcggcaggaa cgcaccaatc tggaacggct
    15121 ctgtcagatg ctgcgggaaa tcgcggtgca gatcgcgttc cgcagtctga cggtgcagga
    15181 actgctggaa cagctttgtc gggaaaccgc ttatgcagcg tttcagtttc cggtgacagt
    15241 gctttccggc atggaacagg ggctgccgct ggcggaggcg tgggcggcag ggatccggca
    15301 ggacaaggca gtgccggaac cggcaaagaa actgctgcta ccgctggggg aagaactggg
    15361 agccagcgac ttggacggac aggcggcaac gctggcacag tatcgcatgc agctggagcc
    15421 ctatgcggcg gaagccggag aaaaatgtgt gcagcggcag cggctgtatt gcagtctggg
    15481 gctgctggga ggactgctgg cggcgatact gctttgttga aagggaatgt ggcaatggag
    15541 atcgatctca tttttaaaat tgccggtaca ggcattattg tggcagtgct gaatctggtg
    15601 ctgaaacggg cggaacggga ggaacaggcg atgatgacaa cacttgccgg attggtggtg
    15661 gtgctgatgc tgctggtaga ccagatcggc accttgttcg aacgggtgaa gtcggtgttc
    15721 gggctatgat cgatgaaacc gtaactgcgg cggcagtctg catcacggcg gcgatctttt
    15781 ccacattgtt gaaacagtac tgtcgggaac acgccctgtt ttgtgtcctt ggagcctgtg
    15841 cggcagtggg cattgcagcg gcggcgtacc tcacgcctat cgtcagccag atgcaggaac
    15901 tctttgcaaa aaccagtctg ccggcggcat atctggaaat cctgtggaaa gcactgggca
    15961 tttgctatat taccggtatc gccggcgacc tgtgtcagga ctgcggcgag tcggcactgg
    16021 caaagacggt ggaactctgg ggcaggctgt cgctggtgct gctgtccctg cctctgctgg
    16081 aaacgctgct gcggactgtg acggaggtgt tgtcatgaaa ttgtggaaag tcctcttgcc
    16141 gctgctggtg ctggggtgca gcctttggtg ctcctgtccg gttctggcgg cggaatctcc
    16201 tgcggaaaca ctggcagagg aaagcggctc tgcggaactc acggagcagc tggactggga
    16261 catacagtcg gtgctggaac agctggggct gtcctttacc gatgttggcg gggcaggaga
    16321 atcctttacc ctctcccgca tctggcaggt gttttccgca tatctgtcgg gacgtatcag
    16381 ccgtccgttt gccatgctgg gagggctgct gacagtaatt ttatttgcgg cgttattgca
    16441 gagcgtacag gacaacgcca ggcaccgcgg caccggacag gtctatgagt ttgtctgtgc
    16501 attggcggcg gtaggcgtac tgaccgagcc gctgtgccgc agctttgccg ctgtggaaag
    16561 tgctctcacc caaggggcgg aattcatgat gctgtttgtg ccggtgtttg ccggaattct
    16621 cgcggcaagc ggcacgctta gcggagccgt ggcataccag actgccgtgg ttctgctggc
    16681 ggacagcgtg atgcagctga ttacccgtgt gctgctgccg ctgtgcacca tgggattttc
    16741 ccttgccatt gtcgatgccg tcagcccgtc ggtgtcggta ggcggtttgc tgcggctctc
    16801 ccgcaagtgc accacgtgga ttctgggact gctgatgacc gtatttctgg gggtgctctc
    16861 catgcagaac tggctgggga gcagtgtaga caccgcggca tccaaaacca ccaagtatgt
    16921 cattgccaat tttgtgccgg tggtgggcag tgctgtttct gatgcctata ccaccgtgcg
    16981 gagcagcctg caaatgctgc ggagtaccac cggcgtagtg ggaatggttt ccctgtgcgt
    17041 gctgtttctg ccgccgctgg tgcagctgct gttgtatcgg ggcgtggtgg cgatcggagc
    17101 cgctgccgca gaactgttcg gcaccaggag cctgctccgg ctgctccgcg gcacccagca
    17161 ggctctggcg atcgcctttg ccctgctggt ctgttttggc gtgatgttcg tggtttccac
    17221 tgccattgcc atgaccattg gaaagggggc ggtataagcc atggaagcca tgcgtgcagt
    17281 ggtgctggtg acctgcgtca tcagcattct tagcggcgtg ttagatgccc tgaaacccaa
    17341 tgcccgattt gaccggcagc tgcggctgct gctgtcggca gtgtttgtgc tgggaatttt
    17401 aacgccgttg agccaaggga cagcagcctt tcagccggac tgggaaccgg acacagaaac
    17461 cgccgcggtg gaactggaag ccgccgccgc acaggcaacc caaaaacagg cggcggcgaa
    17521 tctggaagaa tcgctggagg caatgctgcg gcagcacggc attgccgatg cacaggcaga
    17581 agcggagatg tatatctccg gggacaactg catagagata gaacaagtca cagtgtactg
    17641 cacggatcct gctgccgcag aaccgctgct gacagactgt ctgggaaaag aggtgaagat
    17701 acatgtggaa acagcttctt gaattcctgc ggcagaaact gaaaggggaa aacggtgtcc
    17761 ggctggtggt ctttctgggc attgcaggac tggtgctgat cctgctgtcc ggattcctgc
    17821 cggacaaagc gaaaccgtcc tccaggacgg aaaccagccg gacagaaacc accgcttccg
    17881 acctctccat ggagcagtac tgccggcaga ccgaacaaca gctggtgtcg attctggagc
    17941 aggtcgaagg ggtgggcacc tgtcaggtca tgctcaccgc agacggcacc tccgaaacga
    18001 tctacgcaca ggatcaggaa caggagcagg gagaatcccg caccaagatc cagaagaaat
    18061 gcgtgatcgt ttccgacagc ggcagccagc agcctctggt gcagcaggta gttgcaccgg
    18121 agatcagcgg cgtgatcgtg gtgtgcagcg gagcgtccag tgcagtggta caggagcgtg
    18181 tgacaaaggc agtgcaggca gtgctgcatc tcgccgccag ccgcatctgt gtggttccgg
    18241 cggcatagga aacactgaat aaagcccgcc tgacggcttt gcacgcaatc tgcttgcag
//
LOCUS       DAWBGR010000029        18034 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1051_length_18034_cov_3.988598, whole genome shotgun sequence.
ACCESSION   DAWBGR010000029 DAWBGR010000000
VERSION     DAWBGR010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 18034)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18034)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18034
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1051_length_18034_cov_3.988598"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2121..2130
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16046..16055
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3930 a         4660 c         4966 g         4458 t
ORIGIN      
        1 aatggtataa taagctacat atatttatcg tctgtgtctg tttggaaagc accgattttg
       61 cataagtttt gtgcggtgct gcctttgcac agatgctgac aaaatgccga ggaaggaacg
      121 agtgacatga attttttaga ggaaagaatc gtaaaggacg gtatcgtcaa ggaaggcaac
      181 attctgaaag tggacagctt tctgaatcac cagatggacg tgaacctgtt ccagcaaatg
      241 ggggaggagt tcaaacgccg ctttgcagac aaggagatca acaagatcct gaccattgag
      301 gcttccggta tcggtatcgc ctgtatcgtg gcaagctgtt tccacgtgcc ggtggtgttc
      361 gccaagaagt ccaagagcgt caacattgac ggtgcggtct acgtggcaga ggtggaatcg
      421 ttcacccaca agtgcaaaaa tcaggtgatc gtttcgaaaa agtttctgca ccccgaggac
      481 aaggtgctga ttatcgatga ttttctggca aacggctgtg ccctgcaagg actgatctcc
      541 attgtggcac aggcgggtgc aaccgttgag ggcattggca ttgctgtaga aaagggcttc
      601 cagcagggcg gacgcattat ccggaatctg ggctatccgc tggaatctct ggcaatcgta
      661 gacagtatgg acgcttccac cggcaccatt gtctttcggg aacagaactg aaaggcggcg
      721 gacaaatgga aaaacaaaaa ttttcagccc gcgtgaaaca ggcgatggga gaagcttcca
      781 ttacgaactt gtaccagctg aacggcatgg tgccggtgca taaggcgatt ccctttggct
      841 tgcagcatat cctcgccatg ttcgtggcaa atattgcccc gatcctgatc gtaggcggag
      901 cctgcggact ggcggcttct gatatggcaa tgctgatcca gagtgccatg ctgattgccg
      961 gtatcggcac gctggtgcag ctgttcccga ttttccataa aattggttcc ggactgccga
     1021 tcgtcatggg catcagcttt acctttgtgt cggtgctgtg ctttatcggt gctcaatacg
     1081 gttatggtgc aattatggga gctgtgctca tcggcggcac tgtggagggc atactgggac
     1141 tttttgcgaa gttctggatc aggatcattg ccccgatcgt gtccgccagc gtggtcacgg
     1201 cgatcggctt ttcactgctg acagtcggag caacttcttt cggcggcggc agcggcagtg
     1261 cagatttcgg ctctgcggaa aactggatcc tcggctccgt aacgctgctc tgctgtatcc
     1321 tgttccacat tttcgcgaag tcctacttca agcagctgtc cgtgttgttc ggtctggtag
     1381 tgggctatat tctcgcggtg tttatgggaa aggtcgattt ctccgccctg cgggaaacct
     1441 cgatcattgc ggttccccac ctgatgccgt tccgcatgga gtttcacgct gatgccattg
     1501 tgtcggtggt gctgatcttc ctggtgtctg ccacggaaac cattggggat acctcggcac
     1561 tggctgcctc tggtctggat cgtgacgtga ccacgcagga aacctccggc tcgattgcct
     1621 gtgacggctt tgtcagttcc ctttcctccg tgttcggctg catgccgatc acttcgttca
     1681 gccagaacgt gggactggtg gcaatgacca aagtggtaaa ccggttcacc attgccatcg
     1741 gagctgtaat catgatcctc gccggcattt ttccgatctt cggagcattg ctggcaacac
     1801 tgccggacgc agtgctgggc ggctgtacca ttatgatgtt cggcaccatt gtagtcagcg
     1861 gcttgcagat gatcagcaag tgcggtttta cccagcggaa tatgattatt gccgcactgt
     1921 ccctgagcat cggtctgggc tttaccgaat gtgcagatct gtttgcgatt ttcccccgga
     1981 tggtgcagaa tgtctttgca cagaactgtg ttgccgtggt atttctggtg tcgatcctgc
     2041 tgaatctggt gctgccgaaa aatatggagg cagaaaaggt gaccgaacag aaaaaggaaa
     2101 aggcataaga gcctgtgcag nnnnnnnnnn gtatctggtt ttgtttcaca ggaggtggtg
     2161 caatgacaaa aaagtatacg tatctggtac atattgcttt gtttttggca gcaatgatcc
     2221 tgctctgtct gggctttttt ttggaaaccc ctaatggcgg actgattgca gaacagcgtg
     2281 ccatgccgac ctgtaaagag gtacaggaag tggtagaaga aacccgctct gatgcagcgg
     2341 ctccgcaggg cgtgaccaga gagtttcact ttacgctgga caacaatctg acggaaaagc
     2401 agctgatgtt ttatgttatc aacagcaata ccaaggtcta tatcaacgga gaactggtct
     2461 ttgacaaaca ccgcagcgaa accaataaag tcagtgccac gggctgttac tgggtcaatc
     2521 tgacactgct tcgtgcagac aacggaaagg aagtcactgt agagctgcag ccgctgtatg
     2581 ccgcaactgt caaacgtgct gtgacgtttt atgagggcag ccagtatccg ttgttttgga
     2641 agattttgaa acaggatgca gttttttgct ttttcgggat atttctgatt ttgtttggta
     2701 ttatctatct gggcattgcg ttccactatc gtgtcaaaat gcagatccgt ctgaatcttt
     2761 cggcattggg catggtttcc gtgctggtgg ggtgctggaa aatttccgac acctgtctga
     2821 tcgcatggct ggtgccggca catgtgcggt ttttttccta tttaagcagc attgccgtac
     2881 tactgctgcc acttttcgtg accatgtaca tcaatatttc tgcccgaccg cacagcacca
     2941 ttcagagggt aatgtacggg ctgatgatcg gcaatgctgt gctggcggcg atagcgggtc
     3001 tgctgcacct gctgcggatc gtggcactgc gggaatcaca gggcatgttt gtctatctga
     3061 ttctggcaga agcaattttt ctgatcgtat tggagttctt catttatccc cggcacagaa
     3121 acgacatgca acgcaagctg tttatggctt tggtaatttg cctgatgctg tcgatcttgc
     3181 tggatgtgct ttgctttttg atatggcatt cttccaattt gctgtttttc accatgatcg
     3241 gcattgccgt gtatatcgtg gcaatcggaa ttcttacact tcgcaatgca caggacaagc
     3301 tgttccgtga caaagcgact ggtctgtata accggaacaa gtgcaacgaa ctgatccata
     3361 tgggaatcgc accggatgac cggctgtgct ttatgatgtt cgacctgaat ggtttgaaaa
     3421 aaaccaacga caattgcgga catgatgccg gcgatcagat gatcgcaaag ttcgccgaag
     3481 tgctgcgaaa cagcattccc gcaggcaatt ttatcggcag aagcggcggc gacgagttta
     3541 tcagcattat ctataatact gatgaagaac gggtaaaaaa gatcattgca gatatgcggg
     3601 aacagatcac tgcattcaat caggaaaacg aacacggtgc atggcagctg ggagcagcgg
     3661 tgggctatgc ctttgtgaaa gaggtgggca gccgttccta taaggctctt tatgaactgg
     3721 cagataagcg tatgtatgaa aataaacgtg ccatgaaagc tgtacggaca gagtagtaaa
     3781 ggagttttgt tatgacaaca caggaatttc agaagaagat caaacgcgtc ctgatcacag
     3841 aacaggagat acaggacaaa attcaggaag tgggtgcaca gatcagtcgg gaatacgagg
     3901 gcaagccgct gctgctggtg agtatcctca acggggcatt tgtgtttatg gcagacctgt
     3961 gccgtgctat taccattccc tgtgaaacgg ctttcatgtg tgccaagagc tattacaacg
     4021 gcacatcttc ttccggcatc gtcaagatca ccatggatct gatgcaggac atcagcggct
     4081 atcatgtcat cattgccgag gacatcatcg acaccggcag aaccctgaac gacgtgctga
     4141 agctgctgaa agcgagaaac ccgctttcgc tgaaagtcat caccctgctg gacaagccgg
     4201 aacgccgtct ggtaaagctg gacgcagaca tttccctgtt taccattccg gatcattttg
     4261 tgatcggcta tggactggac tgcggcgaga tctatcgcaa tctgccgtat attgcagaat
     4321 atgatgaagg agaagaagca taatgtttga gaaaattttc cacctgaaag agcataacac
     4381 caatgtcaga acggagtgta ttgccggtgt caccacgttc atgacaatgg cgtacattct
     4441 ggcagtcaac cccagcattt tgagttcgac cggcatgaat cccactgccg tgctgctggc
     4501 aacctgtctg gcatcgtttc tcggcaccat gtgtatgggc atgatggcaa acttgccctt
     4561 tgccctgtct gccggcatgg gactgaacgc ctttttcgcc tataccgttt gcggcaacat
     4621 gggctattcg tggcaggtgg cactgcttgc cgtgttcgtg gagggtatcg tctttattgt
     4681 gctgtccctg acgaatgtgc gggaagcgtt gttcaacgtc attccgcttt ctctgaaaaa
     4741 agcggtttcc gttggtatcg gcgtgtacat cgcctttatc ggcttgcaga acgccggtgt
     4801 tgttgtcaac agtgattcca cgctggtaac agtcgtttcc tttacggaca acttccgcac
     4861 cggcggcatc tgtgccctgc tggcaattat aggtaccctg tttaccgcgt tcctgtacat
     4921 caagcgggtg aagggctcga ttctgatcgg cattctggca acatgggtgc tgggcattct
     4981 ctgccagctg accggtttgt acgttgtaga tccgaaggct ggctataaca gcctgctgcc
     5041 ggcattttcc atgaccgact tctctgcact gggcgatacg ttcgggcagt gcttcaaatt
     5101 caactttgac ggtgtcagca ttgtgaactt tatcgtggtt atcttctcgt tcctgttcgt
     5161 ggatattttc gacacactgg gcacgctgat cggcgtgtgc aacaagggca atatgctgga
     5221 caaggacggc aagctgcccc agatcaagcc ggcactgatg gcagatgcag ttgccacctc
     5281 cgcaggtgca gttctgggta cttccaccac taccacattt gtagaatcct ccgccggcgt
     5341 ggcagagggc ggcagaaccg gtctgactgc ggtagtcact gcggtgctgt tcctgatctc
     5401 catgctgttt gccccgatct tcattgccat tccgtccttt gcgacggctc cggctctggt
     5461 catcgtcggc ttcctgatgt tcaccaccat tccggacatc aaattctccg aagaaagctt
     5521 tctggaagcc attccggcat acctgtgcat cgtggcaatg ccgctgttct acagcatctc
     5581 ggagggcatt tccatcggga tcatctccta tgtagtactg catctggttt gcggcaaggg
     5641 caagcaggtg aaaccgctga tgtatgttct ggcggtgctg ttcgtgctga agtatatttt
     5701 cctttaaatg catctgggcg atacagccct tgtaattgta agagcctgct ttcggtttgt
     5761 ccgggagcag gctcttatgc agtgtttccc ttgtttttca gccgaaactg tgttataata
     5821 gagcatgtac ttgttttgca cttcaaacga aaggaatcgt ctatgtctga aattactacc
     5881 cttacccaaa ctctccgtga tgcgatctct gcccgcattt acggcaaaga agatgtgatc
     5941 gaaaaggtgc ttgccgccct gtactgcggg ggacacgtgc tgctgaacga tattccggga
     6001 accggaaaaa ccacccttgc ccgcaccctt gccggagcac tggacgcgga ctttcaccgt
     6061 atccagttta caccagacct gctgcctacc gacctgatcg gcgtgagcat tccggagccg
     6121 aaaaccggtg cgtttacgtt ccggaaaggg gcggtgttca cccagatttt gctggcggac
     6181 gagatcaacc gtgccacgcc ccgcacccag tcggcactgc tggagtgcat ggaagaacgg
     6241 caggtcactg cggacggcgt aacgtatccg ctggaggagc cgtttttcgt gatcgccacc
     6301 cagaaccctg tggagatgca ggggacattt ccgctgccgg aggcacagct ggaccgcttt
     6361 ctgctctgtc tgtcgctggg gtatccggaa aaggaacagg aacttgccct gctgtcccgt
     6421 cctgcggcag tgccggagag caagccggtg gcaactgcgg cacagctgct ggcagcacgg
     6481 caggagctgg cacaggtgac cgtttctgcc gctgtgcagg aatatctggt ggaactggct
     6541 cacgcctccc gtgagcaccg cgggatacag ctggggctca gcacccgcgg catgctgtcg
     6601 ctgctggaag tttccagggc agcagcagcg atgcatggca gaacctacgt gacacccgat
     6661 gacatcaagt ggatcgccgc cgactgcatg gcacaccgca tgaccgcacg gggcagcatg
     6721 tgggaggaga atcaggaaac ctgccgcagc attgccgaag aactgctgca aacagttccc
     6781 gtgccaaagg aatcgctatg gaactgatcg cagtaatttt gctggtgctg ctggtgctgt
     6841 tcggagaggt gggactgtac cgccggcggg gactggaagg gctgtcctat tactgtcact
     6901 tttcggaaac agaacgcacc gagggcgaat ctctgcaatt tgtggaaact gttaccaatg
     6961 acaaaacact gccggtgccg tggctcaagg cagaactgac cacgtcccgc tggctggact
     7021 ttccggaggc gaattctgct gtcaccggag aaaaccggtt tgtcagcagc tttttctccg
     7081 tccgcagccg ttcccgtgtc agccgcgtct gggagatcac ctgcgaaaaa cgcggggtat
     7141 atgaaatgga acatatcgtg ctggtaacgt cggatttgct gggtatcgtg cggctctctc
     7201 tccatgccgc agaccacggc gaaaagctga ccgtactgcc gaagcggctg accgaagcgg
     7261 ggctgcttct gccgaagctg ctgcggcagc agtacgggga acaggcggtg cggtacagcc
     7321 tgctgacaga tccctgcctg tctgccggtg tgcgggaata tgtgactggt gatccgatac
     7381 accgcatgca ctggaaagct tctgcacatg ccggaacgct gctgatgcgg caggaggaac
     7441 ggacggctcg gcagacggca acggtgctgc tggcactgga atcccaccgc cccgatgccg
     7501 gacagatgac accggacagc gaattgttgg agcacaccat tcgggtgtgt gcccagtgcc
     7561 tctgggaatt ttgcagcaac ggctggcagg tgcggctgtg cgtaggggaa cgcaatgcca
     7621 agggcaaccc cattgccacg ccctacggtg ccggcaatac catgtatcac cggcttttgc
     7681 agcagctggc ggagttgcag ttgcaggacg tgctgtccat gccccggctg ctctcactgt
     7741 ctgccagccg catgacacag gaaaccgtgc tgctgatcac gccctataca gaccggcgtg
     7801 tggcacgctg gaagcaacag tccggcgggt tggtgctggt gacgggacat gcccatgaca
     7861 gcggaaactg tgcggatgcc gtcgttcctg caccggagta tctgcgaaac ggggaggcgg
     7921 cattatgaaa aatatccgct tttggctgca ctggctgctg gtaacggcac tggctgcggc
     7981 tctctatccc tttccggtgc tgcttcgcgt gtggagcaat ggggaacagt ggacggctcc
     8041 ggtgatgacg gcagcccttg caggctgtgc atggctgggc atgctgtata tcctgttgct
     8101 gcggcggcag tacgccagac accccaagtg gatcaacggc ggtgtctggg caatcacgct
     8161 gctgctttgc atggcaggct gtctgttcgc cccctatccg gcaaggctgg cacaggtgct
     8221 gtggggaatt ctggcaggcg gctgcttttt cggcagtgca cggctggtgt ttcacccgct
     8281 ggagcatctg gcacacccga atgtatttgt ggggctgtgc ctgtgggact gcttcaccgg
     8341 attcctgtta tatcttagcc atgccaagct gccctttgtg ccggtgatgc tgctgtttgc
     8401 ggcgaatgcg gcactgtttg ccctgatcca caatcgggac gctatggaac gcatgctttc
     8461 cggtcgggac ggggacacat gggaactgcc gacggagatc cggaagtcca acggaaagct
     8521 gatgggcgtg ctctgcggca tagggctgct tctggtgctg tgcagccgac cgctggcaaa
     8581 agtcatgcgt tgggcgtggc ggctgctgta cacggcagtg tggtacgctc tgcgttggct
     8641 gctctcccgc ggcagtcagg aacagactgc ggaactgccg gaggacacat cggaacaggt
     8701 gatgcagcta cagcaaaacg gtgctgccgg ctggattcgg ctggtgatcg aactggttct
     8761 gctggtggca gtgctggtgc tggtcatctg gaaacgccgc gaaattggcg atgcactgct
     8821 ttccgcgtgg tacagcctgc ggcactggat acagcggcat ctgcggaaaa accacgcgga
     8881 gcagcggaac gaccggcacg aggcgtactg cgactatgtg gaggacttgc tgactgctga
     8941 acagccggct ccgtctgcgg cagccctgta ccgccgccgt gactggaaaa aggcttaccg
     9001 ccggtatcaa cgtttgccgg aacaggcgga acgcttccgg ctggggtacg cgttgctgct
     9061 ggcacggctg ccggaagaag ttgccctgcc ggcagccagt ccggcggaaa ttctgaacca
     9121 tctgtccacc ggtggggaac aggaaacgct gcctcagtgg aacacggtga cagaagccta
     9181 tactgccttg cggtacggag aaattctgcc ggagcatgaa gcatttgcgg caatggatac
     9241 acttttgaaa agacgttcat aggataaaaa aagccagccc acgggaaatc tcccacgggc
     9301 tgactttatt tttatgcagc gttgtcttta ttcgtcgtct tcgctgtgcg ttacctgaat
     9361 gcccagtacg tccagacttt cggcatctac cggagcaggg caggcactca tcagttcgct
     9421 tgcgttctgc accttcggga atgccaccac gtcacgcaga ctttccgcct gtaacattac
     9481 cattgccaga cgatccagac caatgccaag accaccgtga ggcggtgcac cgtagtggta
     9541 cgcctctacc aagaagccga acttggcttc gatttcctcg tcggtcagtc ccagtgcctg
     9601 gaacatcttt tcctgcaact cgtaatcggt gatacgaatg gaaccggagg acagttctgt
     9661 accgttcagc accaaatcaa atgcctttgc agtgacacgt gccggatcgc tgtccagata
     9721 gggcaggcac tcgtccatgg gcatggtgaa cgggtggtgc attgcgtccc agtgctgggt
     9781 gtcctcgttc cattcgaaga acgggaactg ggtgatccac aggaagttga acttgccctc
     9841 cggaatcaga tccagcttct ttgccacttc cagacggagt gctcccagta ccggcagggt
     9901 caccttgttc ttatctgcca caatgagaac cacgtcgccc ttggcacagt ccagacggga
     9961 caggatctcg tccagcttgc cctccggcag gaactttgcg aaagagcagg taggcttctc
    10021 atcgttccag cgaatgaatg ccagtccctt tgcaccgatg ccctttgcct tttcggtcag
    10081 cttgtcgatt tcctttcgtg tcagcacgga agctgcattt tttgccacga ttgcccgaac
    10141 gctgccgccg gcttccagtg ccgagcggaa taccataaag tctacgtcct tgactgtgtc
    10201 agacagatcc tgcaattcca tgccgaagcg ggtatccggc ttgtctgaac cgtagcgttc
    10261 catggcttcc tgataggtca tgcggggcag gggtgtggta atatccatgt gcaggacctt
    10321 ctggaacaga tacttcacaa agccctctgt cagctggaga atatcttcca cgtccacaaa
    10381 ggacatttcc aggtcgatct gggtgaattc cggctgacgg tctgcacgga gatcttcgtc
    10441 gcggaagcag cgggcgatct ggatataccg gtccatgccg gcaaccatca gcagctgctt
    10501 gtagatctgc ggggactgtg gcagagcata gaacttgccg ttgtgtacgc gggaggggat
    10561 cagatagtca cgggcaccct ccggcgtaga cttcatcatc atcggggttt cgatctcaat
    10621 gaagttgttg gcatagaaat actctctggc agccagtgca attttgtgcc gcatcatcag
    10681 gttgtgctgc agcggagcac gccgcagatc cagatagcgg tatttcagac gcagttcctc
    10741 gttggtcttg gtttcgtctg tgatcgcgaa aggcggtgtc tgtgccttgg acagcacacg
    10801 caggtcatct accagcactt ccacagcacc ggttttcatg tccttgttga tgctggaacg
    10861 ttctgccaca atgccctttg ccagcagcac gtattcgctg tgacaggacg cagccttttc
    10921 aaaaatggca cggtcggtct ggtcgttgaa cgccagctgt acaataccgg ttctgtcgcg
    10981 gaggtcgata aaaatcaggt tgccgaggtt ccgtaccttc tgtacgaatc cgcccacaac
    11041 gacggtttct cctgcttttg tcacttcacc gcagtaacag gtgcggcgta aatcctgcat
    11101 caaatccatg atcactgaca ctccttttca aaatcttcca caatgtcgtg gaacatatac
    11161 tgcacggcaa tatcgtcgaa cttgtcctcg aatgtttcgt ccagagcaat ttcgatctgt
    11221 tcgccggttt ccatattctt cagctttgcc tttttgtcct gtagttcgtt ttcgcccaga
    11281 accatgacaa aagcggcacc gatcttgttg gcatacttca tctgtgcctt cagaccacgc
    11341 tgcatcacat catattccac ctgatagccg gaaacccgaa gctgctgtcc cagctgcatt
    11401 gttgtaggaa cagcactttc gtccatgggg gcaatgtaga gatcgcagcc cttgggttcc
    11461 tcaaaggcac agccctgctg ttccattgtc atgatgagcc gttccagacc catggcaaat
    11521 cccattgccg gagtcggctt gccgcccatt tgttcgatca gaccgtcata ccggccgccg
    11581 ccgcagacag tgccctgtgc accgatgtca gtggtgatga attcgaaaac agtcttggtg
    11641 taataatcca gaccgcggac gatcttcggg ttgatgctgt agtcgatgtg cagggctttc
    11701 aggtattcct gcaatttttc aaagtgtgtg cggcagtcgt cgcagagata gtccagaatg
    11761 accggagcat ctttggcgat tccggcacaa atcgggctct tgcagtccag aatccgcatg
    11821 gggttctttt ccagacgtgt ctggcaggtt tcgcagagtt cctctttttt cggttcgaag
    11881 taagctttca gagccttgtg atactccgca cggcagacag ggcagccgat ggagttgatg
    11941 ttgagggcaa tattctgcaa gccggcacgg tcgagaatgg tttttgccag agaaatgatc
    12001 tccgcatcag aagccggtcc ctgactgcct gccatttcca caccgaactg gtggaattcc
    12061 cgcagccgtc ccttttgcgg acgttcgtgg cggaagcagg acaggatata gaacaccttc
    12121 tggggcaggg catcattcag caaaccgttt tgcagcatgg aacgaatggt gccggctgtg
    12181 ccctccggac gcagggtaaa ctgggtttcc tttgcggtga cggtgtacat ttctttctgt
    12241 accacgtcag tggtttcgcc cacggaccgc aggaacagat tggtgttttc aaaagtgggg
    12301 atccgcgttt cgtggaagcc gaagctttct gcggtttccc gtgcaatgcg ttccaccgtg
    12361 tgccacttgt agatctgctt tggggtgaca tcttctgtgc cctgcgggcg tgtgattttg
    12421 atttccatgt atgaaaaacc tcctcgcatt ccgaagaccg gactgcaaaa aatttcccct
    12481 tccgaaaaag gggagaactg tactgtaagt gtctgtccgc cgtggacaga ctaggacatc
    12541 tctttcaggc attcagggtc agatcgacgg atttccgatc tcacgccagt ctaagtcacc
    12601 gcgttccagt gccatgatga gggcttctgc ggtggcgatg ttggtagcga ccggaacatt
    12661 gtgcacgtcg caaagacgga gcagattcat ttcgttgggc tccgaagcct tgcggttgat
    12721 cgggtcacgg aaaaacagca gcacatcgat ctcgttgcag gagatccgtg cagcgatctg
    12781 ctgatcgccg ccttgagagc cgctgagata ccgctggatc cgcagaccag tcgcctctgc
    12841 aacctgcttg ccggtagttc ccgtcgcaca tagggtatgt cgagagagaa taccgcagta
    12901 agcgatacag aattgaatca tcagttcttt ttttgcgtcg tgtgcaatca gagcaattgt
    12961 catataggat aaccattcct ttctacattg tttatcatat cgaaaatgaa aatacaaaga
    13021 tctcattctg tgatttcata attatcagat agtaagacta ctatatcaat tatacaccat
    13081 atcggcaaat ttgtcaaaga gtttccgccg aaaaatataa aattgtgaat tgtatggtgc
    13141 ttcccacgtt ttttcttgtg caatatgacg aaacaaatcc aaatgtgctt taatgtgtag
    13201 tcgtgctggt tgcagaagtg gtggtttccg tgccggtagt atctgtagaa acagttgtct
    13261 gtgctccgac agcatctacg ctgacctgtt cgcccctaac agttttttcg tatgcctgaa
    13321 tgatagagcg aatcatatac ttggagtatt cgccgccttc aatgacgccg gcaaaggcga
    13381 tttccggatc gtctgccggt gcatagccga tgaagaatga atcctgtaca cggttgctga
    13441 cctgcggcgt accggtcttg actgccacat caaagcccag atcagacagg gaataccgtg
    13501 tcgggaaatt gttggtggaa gcggcgatca taccgttttc cacatactgg aacacgtcat
    13561 cgtttaccgg cgtgatctga tcggcaacta ccggttcttt ctgttccagc ttggtggtgt
    13621 ggctgtagtc ccagatgctg tctaccagat gcggttcata gcgggtaccg ttgttggcaa
    13681 tggtgcatgc cacatttgcc atttgcagag gtgtgactgc ccattcgccc tgaccgatcg
    13741 cactctgcaa cacctgaccg acagtccagt cggcacccag ttctgcaaag gtttcctgat
    13801 tggacagatg accggcagca tcgccggttt ccagtccggt ggactgtccc agaccaaact
    13861 tggtggcata ttcagagatg cggtcaatgg tcagttcttc ggacaactta tagaagaaga
    13921 tattgcagga aacccgcagt gcctgtgtca cggcaatgtc gccgtgagaa ccggtacagt
    13981 tgtagtcatg atccttgaaa tgataggtat gaccgcagaa ataggtgctg tttccggtga
    14041 tgatctgttc gtttagacct gccgttgccg taatggtctt gaacgtggaa cccggacggt
    14101 acagaccgtc tgtggcacgg ttgaccagcg gcgtgttttc cgcattgaga atggcttcat
    14161 agtccacctt atagtcttcc agattatagg tgggagcagt taccatgccc agcacgccgg
    14221 cagtttttgc gtccagcact acgattgccc cgcaggaaat ctttttcagc cccagctgtt
    14281 cggttttgga atcccgcagg ctgtcaaagt tgttgataaa gccgtccaga atgttctgca
    14341 attcccgctg gtaatcgctg ttgacagtca gctgtacggt ttttccggct tccaccggtg
    14401 ttttcatatc cgaggaaacc actacaccgt tttccacggt gatctccttg ataccgtctg
    14461 taccgcggag cgtgctttcc atggcacgtt cgataccgct tttgcccacg gtgtcgtcca
    14521 gagcgtagcc ctcggatttc agcttttcgt attcctccgc atagatcggt ccaaccgtac
    14581 cgatctcgtg gggcaccaca tcgccctgtg caacggaacg aagtgcttcc tcgacgatgt
    14641 ccatgccggg gagcgtaaca ccacgttctt tcagttctgt gactacattc agcggcacgt
    14701 cttctgccag tgtaaaacga ttgctgagag agaagccccg cagctgcatt tcataccgga
    14761 ttccggcgag gatccgctgc tgttccggcg tgtagctgtc atcaatgtcg tagtccgatt
    14821 tcagcttgtc catgcagttg tctgcggtgg catagacgtt cattttcaga ttttctttca
    14881 gcttgctgac ggcgttttca tcgtcggtga atgtgtacgg ctgggtatcg gaaatcggca
    14941 gggaagtgct ccactccacg ctgttttcgt ctaaaatact ggtcagcgag agcagtacct
    15001 gattgccctt ggcgttgtcc tcgggaaagg tgtccggctc aataatgaca ttgtagccaa
    15061 ccttgttttc gataatggct ttgccggaag aatccacgat ctcgccgcgg gtggcactga
    15121 tcgtctgggt gtatttctgg gtggagatac gctttgccgt ataggtgtcg ctttccacga
    15181 tctggatttt catcagccgc catgcacagg cacagcctgc cagaagaacc agcatgctgg
    15241 cgagggtgag cttgacatag ctgaatgccg tgtgtttcat agaaaaatgc tcctttccgg
    15301 agagaatgcc tgtgcgatca tgcggtttct tccatgggtt tcagtttctt ctcaatggta
    15361 cgccgccgct ttggcagcag gaaacggtga atcagagaaa agactggata gcagatcatg
    15421 ctgctgatga cggtatacgc cagacagggg agcgtgtggt gcagatatag ctgggaaacc
    15481 tggtgatagc cccagatcgc atactgaaag acgaagtcga aaccggtcac cacaaaggca
    15541 gccactgcag tcagccagat cagattccag aaccgctgca gcagcagacg gtcgtacata
    15601 caggaaactg ccagacagat caggaacaat aagataccgt gatagcctgc cagcgtgccg
    15661 caggaaatat ccagcagcag cccgcacaga atgccgatac cgccggaaac gactgcaccg
    15721 gaaaccgaag aaatgcacag ggcaatggga atcagcagca gcggctttgt aaaatcaccg
    15781 ccgtttgcgg tcacaaaagc cagaaaaatg cagactgcat acaagcccca gcgtatggca
    15841 ttccgcagct tcggacggcg gactggtttc tgggtgagaa actttcgctt actttgccgc
    15901 atcctgctgc tcctttccgg cgaaatcctt gatgacgata acatttttca gattttccag
    15961 atcgacagac ggacggatca cggcacaata actcaagccg ttgtccattg gctcaatgga
    16021 ttccacagag ccgatgagat agcccnnnnn nnnnncggga aaatgcccac agagttggac
    16081 gtggtgatca ggtcgccgct tttcagggtg gtatcctttt ccagatagat catgcggcag
    16141 cggtagttgg aggacagcgt cacatcgccc tccacaatgc cggactccga cttgtccgaa
    16201 gcctttgcac caatggaaag attcggagag cagatggttt ctactgtggc ataggtgtca
    16261 gagatctcgg aaataatgcc cacaagccct tctgccgtga ccaccggatc atagacggaa
    16321 agcccgtcgt cactgccgcg gtcgatgtaa aacccgtgga acggatcgtt ttccacatag
    16381 ccgataatgg tgcaggggtc ggagaactgg ttgtcggggt ggtcttcctt gatgcccatg
    16441 aattccgaca ggtctgccaa gtcctgtttg gtcttgtcat agtccaccat ctggctttcc
    16501 agttctgcca cacgctgacg aagttcctca ttttcagtct ggtacgcttt ggatttggtg
    16561 aatacgtcca gcgtgtcctg cacactggtg gaaatgctgt tggatgccga acggatcggg
    16621 ttcaggatcc tgcccagaaa tccggtagtc ggcagcgtat agccgtcctg tgtgacagca
    16681 tagagcatca tgccgatcag cagggcaatg atgcagagaa tgatgcgaaa ttttttgctg
    16741 cggaaaaagc cgcccatagc tatcagcctc ctttgtcaac cgattactca aatcctctgg
    16801 gatcgtccga aacggcatcg cggtatttgt ccagattttc gagaattttt cccgtgccct
    16861 ctgccacgca gtccaaagga tattctgcga taaacacagg catgccggtt tccttgtgga
    16921 tcagctgatc cagtccgcgg agcagtgctc cgccgccggt cagggtaata ccgtggtcga
    16981 tgatgtccgc agccagttcc ggcggtgtct gttccagtgt gcacttgatc gcatcgacaa
    17041 tgcgggaaag gggttcagag agagcctccc gcacttcttt cgaagaaatg ttgacatttt
    17101 ccggcagtcc gctcatcaga ttccgtccct tgatctgcat gatctcgtca cggtcaacag
    17161 gatacgcgga accgatctgc atcttgatgc tttcggcagt ccgctcgccg atgagcaggt
    17221 tgtaccgctt tttgataaag ttgatgatcg ctgcatcaaa ctcatcgccg gcacagcgga
    17281 cgctctggga aacgacaata ccgcccagtg caatgacagc cacttcagaa gtgccgccgc
    17341 cgatgtccac caccatacag ccgataggct cggtggtagg cagtcctgca ccgatcgccg
    17401 ccgccatcgg ttcttctacg atcagaaccg gtttcgcccc agcctgatct gcggcttccc
    17461 gcactgcacg gcgttccact gccgtgacac cggagggaat gcagatgatc actctggcac
    17521 gggcgaacat tttgccccgc atggctttcc ggataaattc ctgcaataaa atggttgtgg
    17581 tttcaaagtc cgcaatgaca ccgtctttca gcggacgaac ggcaacgatc gagccggggg
    17641 tgcgtccgat gacttccttt gcctctttgc cgacatattt tgcacggtct gtgtgggtat
    17701 tgactgccac gacagacggc tctctgataa taatcccctt gccacgcagg tagacaaggg
    17761 tgtttgctgt tccaagatca ataccaatat cttttgtaaa cattgtgtgc tccttttgat
    17821 gcgtgtccag ccgcatcttg ccccgtggta aaaaagcctg ccaattcgcc tctttccgga
    17881 gaaaagagaa cactgccgcc gaagaccggt gcagtgaaga atctgtattc ataaatatgt
    17941 atacttgttt ctttttatga atactgcttt tgaaatttca ggcaataccg cacaaagatt
    18001 gtgcgacaga tgcgtcgtca aattttttgt gaaa
//
LOCUS       DAWBGR010000030        17749 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1073_length_17749_cov_6.732791, whole genome shotgun sequence.
ACCESSION   DAWBGR010000030 DAWBGR010000000
VERSION     DAWBGR010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17749)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17749)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17749
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1073_length_17749_cov_6.732791"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    17072..17081
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4261 a         4661 c         4471 g         4346 t
ORIGIN      
        1 gccctgcggg catctcccca ccccgtgggg agtcacccct ttcaggggag gctattatga
       61 aaattgattt ccgtaggatt cgcaaaatcc ctatggacaa atactgaaaa atatgctata
      121 ctagatgtaa caaatcatct tatcaaagga gtcgggtgaa acccatgcag catgaaaaat
      181 cctgcggtgc cattgtgtac cgcaaatccc acgggaacac ggagatcctg ctcatcaagc
      241 acatcaacag cgggcactgg tcatttccca agggacacat ggaaaacggc gaaacggaaa
      301 ttgaaacggc aattcgtgag atcaaagaag aaacctctgt caatgtcatg atcgatccca
      361 cctttcggga aacggtttcc tactttccca agcgggatac acaaaagacc gtggtctatt
      421 ttatcgcacg ggcaaagaat tacgactttg tgccgcagga agaagaaatt gcagagatcc
      481 gctgggtaga catcggatac gcctctacca ttctcaccta tgagaatgac aagaacattg
      541 tcaacaaagc aaaagcagcg atcaaagaat tctgaacact ttacaaaaat caaaaccgct
      601 tccgattcgc attgcataga accggaagcg gttttttgag gaaacactga aggcaatacc
      661 gcacaaagag tgtctggcga ctttgcctga ataaagcttg tgcagtgctt cctataaaat
      721 attccaccag aacggcattt cgctttcgcc aagccgcgtg atccacatac gcacttcttc
      781 cagttccgag gtcagttcgt cacagtcctc tgcctgcatg ggtgccagat gcgaaactgt
      841 cacatcgatt tgcagcacct tgtcccgcca gaccaccaga ttcagccggc gttccgattt
      901 ttcccactgg ttttggaact ccgcaaatgc ctgcttcgcc tgctccggct gggtttcggc
      961 aatttgctgt acggtgtcca gctgtttcag caggcggctg gtttccgaac gcaccagcca
     1021 cgctgtcccg cagctgcaca gcaccagtgt cagcagaatg gcaactccca gacgtacccg
     1081 attcatgccg cgtccccccg tttctttgga ataatggtac aggtgccctc ggcatcgctg
     1141 agcatcagga acacctgttt ttccgtcagg cggtgctccg ccagtttctg ttccagccac
     1201 gcctgatttt tccccagcat ggcaagggac ttgttcatga cgcagccgtc ctgtatcacg
     1261 gcaacaggcg gattctgtgc cgtctttttc agggtgatgt ccgccggtgt cagaggctgg
     1321 cactccgcct tgagatagac ggaaatcgtg ccggtggttt ccatgacggc gaactgcacc
     1381 tgactgatgt caaacacctg ctgctgccgg agagccgtca tcaggtcatc taccgacagc
     1441 cgcagatccc gcatggtcgc ctgttcgatc tgcccgtccc gcaccacgat ttttggtctg
     1501 ccggaaatga tgcgacgcat tcgcagggat ttcagcgtca cccacgacat caacgcttcg
     1561 aagcagacca gcgtcaggat cggcaggatc cccagtatca ggggaatgct ggtatcctcc
     1621 agcggcaggg tggcaatgtt ggagatcaga atggtgatga ccagctccga gggctgcaat
     1681 tcccccagct gccgcttccc catcagacgc actccgaaaa tcaccacaat gtacaagatc
     1741 agcgaacgaa tgagaaccac actcacaaaa aacgcctcct ttcatacgag tagtatgtcc
     1801 ggaggtgttg gagattatgc ggttgctctt aggcaatacc gcacaaagag cgtctggcga
     1861 ctttgcacgc catctgcttg caaatttccc gtcatatttc acaaaaatgc tcgtgaatac
     1921 atccagtatt cactccgctt tttgtttcat ctgacaaaaa atttgacgac gcatctgtcg
     1981 cacaatcttt gtgcggtatt acccttattc tgctgtttct ttttctgctg tttctttttt
     2041 cttgaagatc accagcttgc tgaaaatgta gttcagcacc agaatgacta cctgtgccag
     2101 aatcttcacc agcagcgaac gccatgtgac gatctcgccg ttgacggtgg cgaagaagtg
     2161 gttgcccagt tccataatgg caagctccag aaaataggtc accagacggg caccgtagaa
     2221 catgtagaat tcccgccaga acgccgccct ggtgtgggtt tcgcttttga acacgtactt
     2281 cttgttggtg aagaaggcaa acgtcaccgc aaagatccac gagatcgtgg tggaaagtcc
     2341 ggtgttcagc cagttcgcca tatgcatggt gttgaacagt gctgtcggca gcatctggaa
     2401 caccagcgaa actactgtag tcagaccgcc catgagaatg tacatccaca cggattcgtg
     2461 gtcgtaccac agcttttgca gcggcttcgg caaatggtcg atgatccaat gaattgcttt
     2521 ttccatagaa gattcccctt ttcgacaagt tttcacaaaa attgtggttt cccttgattt
     2581 tttttaaatt ttatggtatg atagaagaat caatgattct acaggcagca ccgcacaaaa
     2641 cttgtacagc agtgttgccc acactttctc gggaggaact attttgggtt acgtcatacc
     2701 aattctcatt cttgttgctt tttccgcact gttttccagc acggaaactg ctttcgcctg
     2761 cgtcaacaaa atccggttaa agcacatggc agattccggc gacaaacgtg ccgaaaaggc
     2821 tctggacatc gcgaaccgct atgaacaggc tctgactgcc attctggtgg gcaacaacgt
     2881 ggtcaacatt gctgcatctt ctctggcaac cattctcttt accgcgtatt tcggctctgc
     2941 cggtgcagcc atttccacag tcgtcatgac gattctggtg ctgacattcg gcgaggtgct
     3001 gccaaagtcc tacgccaagt cccatgcgga caagctggct ctggctctgg gcggcattct
     3061 ctccaagttc acactgctga tgacgccgtt tgtactgttc ttcggtttgc tctcccgtgc
     3121 gttcaccgcc aaagaggaaa cgcccagtgt caccgaggac gaactgaaat acatcattga
     3181 cgagatcgag gaggaaggcg tactggaaga acaggaaagc gatctggtca tttccgccct
     3241 ccagtttgac gaaacttccg tcagcgatat tctgatcccc cgtgtccgca tggtagctct
     3301 cccctcggat gccacagtgg aggaagcaaa gcagctgttc ctcaacaacc acttttcccg
     3361 ctttccggtg tacgaaaagg acatggataa catcattggc atgatcacca acaaggactt
     3421 tttccggctc atgtgcggcg aatttacaga gctgtcagaa atcacacaga acgtcatcta
     3481 tgtaccggaa accaaacgca tcagcgatat tctgcgggac atgcagcacg ccaagactca
     3541 ccttgccatt gtggtagacc agtacggcgg caccaagggc atggtcacac tggaggacat
     3601 cattgaacag ctggtgggtg agatctacga cgagagcgac gaagtcattc acgagttcca
     3661 gaagacggct ccccacacat acaccattct ggggtcgttc agcatcagcg atgtctgttc
     3721 cgcactggac gaggaggacg ctcctgcaaa ggttccggaa accgactgca ccaccatcgg
     3781 cggctgggtc atggaactgc tggggcacat tcccgatgcc ggcgaaaccg tagagcacga
     3841 caacttccac ttcaccgtac tggaagtgga aaaccagacc attcagtcca ttcggctgga
     3901 ggtcacaccg ccgcccgcag agaacggcga atccaaataa aagcctgaca gcgaggcagc
     3961 cggaaaaccg ttccggctgc ctttttttgc gttgccgccg ctatttcttc ttatacttga
     4021 acaggacaaa tgccacgatc gccgtcagca gcagtgacag cagcgtggga aaccatgtat
     4081 gctccgcaaa gggcagatcc agcgtattca tgccataaaa gctatagacc atcgtcggga
     4141 tcgccagaat gatcgtcacc atagtcaatc gccacatgac gatattcaga ttgttggaaa
     4201 tgacagatcc gaatgcgtcc accacgccgg acaggattcc ggagtaaatg ctcgccattt
     4261 cctgtgcctg tttcaactcg atcagcacat cttccagcag atcctgatct tcttcgtaca
     4321 gcttcaccac tctgccgcgg aggatcttgt tcatggtcac atcattggat ttcagcgatg
     4381 tggagaagta gatcagggat ttttccagtt ccagcatctg aatcagttcg tgattcttca
     4441 tggcaccgtg cagtttccgc tgcatggcac tggaagtttt gtcgatttgt ttcaggtata
     4501 ccagaaaaga tgcggtgatg ttcatcatca gccgcaggac aaactgtgtc cggaactgag
     4561 tctggatatt ccgcacccgt ccgctgacta catcattgat gaccttgttc tcccgcagag
     4621 aaatggtaaa aacataatga tcggtcagga taatgcccag aggcatggta taaaaaatca
     4681 gcgtgtcgtc cttctgcact tccgaaacgg ctgtgtccac gatgatcagc gtctggtttt
     4741 cctcctgctc cacacgggac gattcttctt cgtccagtga ggatttcaga aagtcgctgt
     4801 ccagaccgta atcgtcgatc agcattttcc gttcctgtgc tgtggggtct accacagaga
     4861 tccagcaccc cggcatcaga gaatccacct cgatcaaccg gtttgccacg gttttataat
     4921 aagaaaccaa atcggatacc acctttcccg caaggcagcc cgaccggtgc cggcatcagc
     4981 tgtccttgct cattttccgt gtgttcatcc gtttgccaaa gtcatatcgc gtacatggca
     5041 agggataagg gtcagcgttc atgccgcacc tcctagtgtt atcatattcc ggcaaatttt
     5101 ccggcgtgtg catcacgcgt caatttgccg cattttctat tataacagaa aaaaccgcct
     5161 ttgacaagac ggttttttgt aaaatttgaa acaactgaaa caacaccgaa cgagatccgc
     5221 cggacagcgg acgggttgcc ttaaatacag taatcatctc cgcagcccgg tccgctgttc
     5281 aaaatgtcct gtagtgtgat gctgtccaga taacggttga ctaccttata cagtccttcc
     5341 cacacagaga tcgtcaggca ctgttccgca cggggacagt ccaccacacc ggtttgcaga
     5401 catgccactg gtgcaaggtc gccctctgtg atgcggagca cctcgccgac agtatactcc
     5461 tccggagcct tggagagccg gtaaccgccc tgatagccgc ggttggtgcg gagcatattg
     5521 gatttgttca gcagtggaac gatctgctcc agatattttt tcgaaacgtc ctgccgctgg
     5581 gcaatgtctt tcagcgaaac gtagccgtcc ccctgattct gtgccagatc cagcagcatg
     5641 cgtaaggcgt atcgtccttt ggttgagatt ttcatgtgtc gtcctttcca gcgtgacagg
     5701 cagcatttac acgccgtgct gcctatactt ttttatcata tactactatt ataccatagg
     5761 ttttgtaggt tttcaagggc aaaacagcaa tttttttcaa aaaggtgtac aaaacaacgc
     5821 tgccgaaatt gtggaaaacg acgaaatacg cctttgtgcg gtattgcctc aggacttttc
     5881 cgcactggaa attctgccgc cgcccacaac ggtttcgccg tcatagagaa cgacagcctg
     5941 cccgcaggtg atcgcccgct gcggctcgtc gaattccaca tggagtacgc cgtccggtgt
     6001 gcaccatgcc gtggcaggct gttccggatg gcggtagcgg atcttcgcct gtacgcgaac
     6061 cggcttttcc agactgtcgc aggagatcag gttcacgtcg gtggcggtca gcctgcggga
     6121 aaacaagtcc tgactgccgc ccagcgtgac ggtgttctgt tccggacaga tgccgcagac
     6181 atacagcgga tgcggggcag aaatgcccag cccttttcgc tgtccgatgg tgtagtgaat
     6241 gacaccacgg tgggtgccga tcggttcacc ggctgtgttg agaaatgcac cggtccggga
     6301 cgttttgccg gtgtagcgtt caatgaatcc ggcatagtcg ccgtcgggaa caaagcagat
     6361 gtcctgactg tccggcttgt ccgcgttgat gaagccgttt gcctcagcga tctcccgcac
     6421 ctcgtgcttg gagagttccc ccacagggaa ctgggtgtgt gccagctgtt cctgtgtcag
     6481 attgtacagg acgtagctct gatccttgct gttatccagc ccctttttca gtagataccg
     6541 tccggaagct tcatcgtaga ccacgcgggc atagtgacca gtaacaatgg tgtccatctg
     6601 catttcctgc atccgcagat agagcttttc aaatttcaag taccggttgc agtcgatgca
     6661 ggggttggga gtccagccct tttcgtaggc acagacgaac cgatccatga cctccttttc
     6721 aaagtttgcc gtgaagttga aaacatagta gggcatgtgc agccggtaag ccacgctgcg
     6781 ggcatcttcc acatcgtcca gagaacagca ggtcttttcc cgctttacgc ctgcctgttc
     6841 gttttcgaac agcttcatgg tcgccccgac gcattcgtag cccttttgct gcatcagcag
     6901 agccgccacg gagctgtcta caccgccgct catcgcaatc agtgcctttt tcatacgatc
     6961 acctttttcc tagaatctta tattatattc agtataaacg agaacggggg aaaagtcaag
     7021 ggcaataccg cacaaagagc gtctggcgac tttgcacgcc atctgcttgc aaatttcccg
     7081 tcatatttca caaaaatgct cgtgaataca tccagtattc actccgcttt ttgtttcatc
     7141 tgacgaaaaa tttgacgacg catctgtcgc acaatctttg tgcggtattg cccaaggaaa
     7201 tgtggaaaat catctgacag gagcgtttga cttttcccgg aaaagtgtta tactggaaag
     7261 tatgtagaac gtaacagaaa aggagaatgt atcaatgaaa cgttttttgc aatggtggaa
     7321 aggacagccg gtgctgggga tctcgctttt ggcggcgctg gtgtccatgt gctttgtgcc
     7381 gccggatgcc ggctatttgt cctactgcaa ccgggctgtg ctgattcagc tgttttgtct
     7441 gatgctcacg gtggcaggac tgcgcagcat cggcgtgttt gaacaggtga cgcaaaactt
     7501 gttgaaacgt gccgggacgg tgcggcgtgt gggaatgttc ctcgttcagc tttgtttttt
     7561 cagtgcaatg ctggtgacca atgatgtggc gctgctcact tttgtgccgc tgacactgct
     7621 gctgttccag gaaatcgggg atgagaaaag ccgcatctgg gtcattgttc tggagacagc
     7681 cgccgccaat ctgggcagca tgatgacacc ggtaggcaat cctcagaacc tatacctcta
     7741 tgcggcgtat cagctgcata ccgcagactt cttccgtacc atgctccccg ccggcgtgct
     7801 gagctggctg atccttctgg gactgtcgtt cctgctgccg aaaacgccct gcgccggaaa
     7861 gcctgtggaa acggcttcgt ctgccatacc gaaaaagccg gcggcggtat atctgctgct
     7921 gtttctggcg tgtctgttga ctgtgttccg ggtgcttccg gactggctct gccttctgct
     7981 gactgctgta ctggtatttc tctgtgacaa aaagctcttt gcccaggcag attactgtct
     8041 cctggggaca tttgtctgct ttttcgtgtt cgtgggcaat attgcacggg tcgatgtgat
     8101 tcgggatttc tttgccggcg tgatgcaggg cagggaactg tgggtctcgg cagcactttc
     8161 gcagtgtatc agcaatgtgc cggcggcggt gatgctgtcc ggctttacag aaaatgcaaa
     8221 tgcactgctg ctgggcgtga atctgggcgg ccttggaacg cctatcgcat ctctggcgag
     8281 tctgatctcc tatcagtttt acagtaaggc agtgcattcc cgtccgggaa aatatctggg
     8341 cattttttct gctgtgaatt tcgggatgct ggcgattttg ctggcagtgg gatggctaca
     8401 gctgcgatcc ttctaagcgg attccctcgt atgccagaca atgcgcctcc ggctgatgcc
     8461 ccaattctgt ggtcacggca atgggcagcg caggatcgtc tatgatgtcc tgcaccaacc
     8521 ggtacagcgt cgggagctgc tgcttttccg ccagttccga aaagttcccc agcaacacgc
     8581 cgctgcatcg ggagaacatt tccatttgcc ggtattggtg cagcatagtc tccattcgag
     8641 caaaactgcc ggaatagctc tccagaaaca gtacccgtcc cgtgggatct ggctgccacg
     8701 gtgtgccggt gagcttcagc gtgcagcgga tattgccgcc gcagaccctt ccggagagcg
     8761 aactgccctg caaaaaggca tagtgaaatc gaaacagggc atcgtcctgc tgctgtatcc
     8821 agcgcgcaaa ccgttctttg gcggcggaac agcttgccag aaagcgcgcc tgcaaataaa
     8881 acaccgggat gtctccgaaa tcctgcagcg gattgagcag aaccgagacg tcgctgtacc
     8941 caaaaaaggg ctttgagcat ctggcgatct ctttaaaatc gagatggggc agaatggcat
     9001 ttgctgcgtc accgccggtc agatcgaata aagcatccac ttccgggtct gcaaacgcgg
     9061 cttgtatgtc tgcggcgcgt cgttttgccg gctgcggctt gccggcagca gtctggtata
     9121 gggatggaaa aatcacggtg tgcagtccca gtgattccag aacagcacaa agacgcgaaa
     9181 ccgtatcact gtcccggggc agcccgtcgg aactgcccac cagtgcaatg gtactgcggg
     9241 gagaaagctg taacatgaaa agacctccgt tcttatttat aaggcaatac cgcacaatct
     9301 ttgtgcggta ttgccttata aatctgcttt agtataacac atcagatcgt caccgtcaaa
     9361 gctgaaattg gacctacaat tttacaaacg aaacaggaaa tgcgattttg cttttctttt
     9421 ttttaagagg aatccaacaa atcgccggaa aagatcaaaa agtaaggttt tgcaaaaaaa
     9481 aatcttggga atttgcaaga aacctattga catcgtagtt gagatgtgat attattaaga
     9541 cagatcaaaa cacagtcctc tgacggcaaa aaacacgtcg tgcaagcgtc gtgaaaattg
     9601 cctgcccaaa agattgtgct ttggaaaaat ccaaatcaaa tttcgccggt ttatccggca
     9661 aaggggagat cttaaaatgg cagagacaat ggcaaaagca tgggaaggct tcgttggcgg
     9721 tgcttgggaa aagaccatcg acgttcgtaa cttcatccag aagaactaca ctccgtatga
     9781 gggtgacgaa agcttcctgg ctggcccgac agaatccaca acaaagctgt ggagcgaagt
     9841 aatggatctg ttccagaaag aacgtgaaaa cggcggcgtt ctggacatgg ataccaagct
     9901 ggtatcgaca gtagattctc acgaagcagg ttatatcgac aaggatctgg aaactatcgt
     9961 aggtttgcag actgacaagc cgctgaagcg ttctctgcag ccgttcggcg gtatccgtat
    10021 ggctgaagaa gcatgcaagg cttacggcta tgaagtagat ccggaaatca cctatatctt
    10081 cacacactac agaaagacac ataacgaggg cgtattcgat gtttacacac cggaaatgcg
    10141 tgctgctcgt cataatgcaa tcatcaccgg tctgccggat gcttatggtc gcggccgtat
    10201 catcggtgac tatcgccgtg tggctctgta cggtatcgat cgcctgatcg aagacaagaa
    10261 ctaccagaag gcaattactt ccggcaccat gaccgaagaa aagatccgcc tgcgtgaaga
    10321 actggcagac cagatcaagg ctctgaaggc tctgaagaac atggcaaaga agtacggcta
    10381 tgatatttct gttccggcta ccaatgcaaa ggaagcaatc cagtggacat actttgcata
    10441 tctgggtgct gtcaaggatc agaacggtgc agcaatgagc ctcggccgta cctccacatt
    10501 cctggacatc tacattcagc gtgatctgga caacggcact ctgactgaag aacaggctca
    10561 ggaatacatc gaccacttca tcatgaagct gcgtatggtc aagttcgcaa gaactccgga
    10621 atacaacgca ctgttctccg gcgacccgca gtgggttact gaatctatcg gtggtatggg
    10681 catcgacggc agaactctgg ttaccaagaa cagcttccgt tatctgcata ctctggaaaa
    10741 tctgggtact gctccggagc cgaacctgac cgttctgtgg tccaagcgtc tgccgaaggc
    10801 attcaagcac tactgtgcaa agctgtccat caacacctcc tcgttccagt atgagaacga
    10861 tgatctgatg cgtgtgacac acggtgatga ctatgcgatc gcttgctgcg tatcctccat
    10921 gcgtgttggt aaggaaatgc agttcttcgg tgcacgtgca aacctggcaa agtgcctgct
    10981 gtacgcaatc aacggcggtg tcgatgaaag actggacgct aagaccggca agcacaatca
    11041 ggttggtccg aagtatcgtc cggtagaagg cgattatctg gattatgatg acgttatggc
    11101 aaagtacaca gacatgatga catggctggc taagctgtac gtagataccc tgaactgcat
    11161 ccactacatg cacgacaagt actgctacga agcaatccag atggctctgc acgacagaga
    11221 tgtaaagcgt tatttcgcaa ccggtatcgc tggtatgtct gttgttgctg actccctgtc
    11281 tgcaatcaag tatgcaaagg taaagtgcat tcgtgacgaa tacggcgttg taactgactt
    11341 cgaagtagaa ggcgacttcc cgaagtacgg caacaacgac gaccgtgtag acagcattgc
    11401 tgttgagatc ctggacaagt tcatggatat gatccgcagc aactactgct acagaggcgg
    11461 cgttccgacc acttctatcc tgaccatcac ttccaacgtt gtatacggca agaagaccgg
    11521 taacactccg gacggccgta agaagggtca gccgctggct ccgggtgcaa acccgatgca
    11581 cggccgtgac actcacggtg cagcagcatc tatggcatct gttgcaaagc tgccgtggaa
    11641 gaatgcacag gacggtatct ccaacacctt caccatcatt ccggatgctc tgggtaagga
    11701 ttccaaggta ttcgctggtg atattgacat cgcttctatc cgcgaggaaa tggaaaagca
    11761 gtaagtctga aaaggactat ttgaaaggag gcacagggtc atggcagaac atgaagtcaa
    11821 ggaacaggat aagtcggacg aactggtgga catgattgaa aagctcatgg aagatggcag
    11881 cggtcatctc acgatttccg aagacgatct catgggcgag ggcttgaatg tgaagaccta
    11941 tcgcagcatg gattgctcga aggggaacat ggcttgctgt cagccgactg aatttataga
    12001 ggacgagtga atttttcacc agtcccgcgt atatcccgat ttgtaaattc tcaaggagga
    12061 atttatcatg gcaaacgaaa agcaaattga aaatctggta ggtctgttgg acggctatgc
    12121 agaaaaaggc ggacaccacc tgaacgtaaa cgttctgaac cgcgaaactc tgctggacgc
    12181 tcaggcacat ccggaaaagt atccgcagct gacgatccgt gtcagcggct atgctgttaa
    12241 cttcatcaag ctgaccaagg aacagcagga cgacgttatc accagaactt tccacgcttc
    12301 tctgtaagcg gaaacgtact gaaacagcaa aattagcata acataatgtt agcagtgggc
    12361 gtgcagtttc tgtacgccca ttgttgtatc aaaatgatcg gagtgcggca gaatatttgt
    12421 gaaatgctgc atttcaggaa aggagtacaa tatggaagga cacattcatt ctaccgaaag
    12481 cttcggcacc gttgatggcc ccggcattcg ctttgtgatt ttctttcagg gctgccccat
    12541 gcggtgtcag tactgccaca atccggacac gtggacgatc gacaacagca acaccgaaaa
    12601 tctgcgttct gtggaatccc tgttgaaaga gtatgacggc atcaaggaat ttctccatga
    12661 cggcggcatt accgccaccg gcggcgagcc gctggtgcaa atggagttcc tgacagaact
    12721 gtttgaaact gccaagaaaa agggcatcca tacctgtctg gacacctccg gaatcctgtt
    12781 ccggcaggaa cgccgcgagg aattcgaccg ccttatggca gtgacagatc tggtcatgct
    12841 ggacatcaag cacattgacg acaccgagca ccagaagctg accaagcaga gcaacaagca
    12901 cattctggaa ttcgcggagt atctgaacga aaagggcgtt cccgtctgga tccgccacgt
    12961 ggtcgtgccg accattacgc tgaatgatac ctatctgaaa cagctggggc attttatcgg
    13021 cggactggat aacctgaaag ctctggacgt gctgccgtac cacaccatgg gcgaagtcaa
    13081 atacgaaaat ctgggcatcg actatccgct gaaaggcatt ccggcggcga ccaaggaaca
    13141 ggcaatccac gcaaaacagg tgattttcga gggcatcaag gaacggattc gggaagatat
    13201 tcacaagaag tgaacgaaaa aatgcccctg aaagcgaaag atctgcacgc agatcgcatg
    13261 caatagaaac aacaaaagcc gcatcacgga gaaagttccg gtgatgcggc tttttgcaat
    13321 accacacagt tacactggca tagaattatt ctgcggtttg atgacaatgc ggttcttgtt
    13381 tttcttatcc tgatatagct tctgatcggc gtacttgacg taattctgca cctgttcccc
    13441 gccctgacat gtcatggtat acacaccgac actcagcgtg atgttatagg gaatggcact
    13501 ggtggcgtta tactgctggg cagccttttt gatgctgtcg tagatctgct ttccggcagt
    13561 ttccggatca tcgaccatga cgaagccggc aaactcgtcg ccgcctactc tgccgatcgc
    13621 gtccagcagc ggcgttgcag aactgagata ctgcacgcag gttttcaggg catggtcgcc
    13681 gccgtcgtgt ccgaatgtat cgttgatgat cttcagattg tccaagtctg ccagaatcag
    13741 cacgcagcct ttcccagcgg attcctgact gctcagcatg gcatttgccg cacgatagaa
    13801 gccgcggcgg ttgtacacac cagtcagttc gtccgagtcg gagatcagct tcagacggct
    13861 gttggcgaat cgtgcttcct ccagattgcc ctccagttcc ttgaccagac gcgtcatctt
    13921 aatggcggag cagatctgcg gtgctacgga atagatctgg gagaaatggt tatagtccag
    13981 ttcacagata aacagcccgt actgctcctc gttggagaac agcggcacca tgacaaacgt
    14041 ataccgccga tcctgcggca tatagcggtt ggcaatgcag gagtaaacgg aaacctgctg
    14101 ttccggcggc tctacccgct gtaacttctg cccgatatgg taggatttca ggtacaggtt
    14161 ttccggcatg atccactgct catactgata gtgcaccacc ggcgaatgga acacgtagat
    14221 atagctgctc ttgaagctga cccgatagag gtttttcaca atggagaaaa agcttttttc
    14281 ctcgtcattg ctgttaatca tcatgtcctt gttgatgttg ctgatcagga aattgctgta
    14341 caccagattg gtgcggagca gatactgctg ctgcatgctg tagtccagca ggttatccag
    14401 aaagaacgca tggagccgtt ctactgccag cagattttcc ggctgttccc gacagatctg
    14461 cacggcaaat tctttcgcat gcgtcacaac ttttttcagc agcggcacct gtatcggaat
    14521 cacgtccgtc tggtcgaaca tctcggagat ttccttctgg tattccgcca gcggcagcgg
    14581 ttcatggctg caaactgcct gaaattcttt tatcagcagt tcccacaccc gctgaatgaa
    14641 tctggtcttt gtcgtgctct ccagatcgcc gatgtacttg ttgaagatca tacccacaat
    14701 ttcttccgcc gcacagtgat ctgccggatt ctggatctgt tcctctgtca ggtcgcagga
    14761 ttcccgcacg atcagccgtg cgtccagcgt cctgctccgg gttttccctg tctggatcag
    14821 ttccaccaaa gcctgcaccg actggtagcc catacttctg atattggtac gcacggtcgt
    14881 cagcatgggt ttcagactgg tggcaacttc ggaatcgtca aagccggtga ccagaatatc
    14941 ctcgccgaca cgcattttcc gttccttgaa cacctcatag ccgccgatgc acatcttatc
    15001 attggcaaag cagatcgcct ctagatcggg gtgcagatcc agcagattcc gcaccacttc
    15061 ttccgaagcc tcggagaagt tgccgtatac caccttggtg cagtcatacg ggatatggtt
    15121 ctcctccagc actgcacggt agcattgcag acgctccatg gcatcctgat ttcccatagg
    15181 accggaaaca aagccgatct tccggcagcc atggtggtgt atcagatgtt ctactgccag
    15241 cttcatggcg ttggtattga accgaatgca ggggtatccc gggaattcca cttccatagt
    15301 cagcagaggc agcggatcat aggagtggag aaactgtgcc atttcctccc gactgatata
    15361 ggagccgaca gtgcctaccg acaccagcag accgtccaga ttcctttcag aaacatagtt
    15421 gtagatcacg ttgtactgat aatcatagat cgagtatttt tcatcatctt ctactgcatt
    15481 gatgcctctg cccggcagca ggatcaggtt gatgtccagt tcttctgccg cagcaatcgc
    15541 acccttgaga atttcgcttg catattcatt ttccagatgg tgtatcatca gaccgatctg
    15601 gagccttttt ttctcagtca tgattgctct cccctgtctt ctatccaaaa ctccgcttgt
    15661 cattgcgatg aaaccacaca aaatgatttg tgcggtgctg ctttatggga atattatgaa
    15721 aggcaatacc gcacaaagag cgtctggcga ctttgcacgc catctgccgc acaatctttg
    15781 tgcggtattg ccgaaagcat tgtagcattt gattgcatgc attctgtatt catgcacttc
    15841 tgtagtataa cacatttgat ttggaaatgc aagagttttt tcacatttta ttttcatgaa
    15901 aataatgatt aaattgcata atttaacttt cttattacac ttcattttcc agcgtatagc
    15961 ggcattcggg atagttggca cagccgtaaa aagagccgta tctgccctgc cgcagcacca
    16021 gctttccgcc gcaacgggga caaagtcccc tccgcagggc aaaccgccga taagcgatct
    16081 cgtcatcgat ctgggagagg tgctgttccc gtgcctgcgg gtcctcgatg ttggcacaca
    16141 aaatcacgcc ggcataatag cggacatcgt cccacagcag caggggctgt ttccggcggc
    16201 ggacggcacg gcacaccttg gaaatagaca ccagctgttc tgtactgtct tccggctgta
    16261 ccacctgtaa gacggcatca tctgcgaacg ccacaacgga aaagaaacag tcccgttcca
    16321 gccccagcaa actggcaagt gcctgcacat gagcgtaatt ctggcgaaag ggattccgca
    16381 ggtcatactg cctgccgtga cactcctgca cccagaactc tttccagatg ctgccggaaa
    16441 tggtgccgtg ataattcttg gtttcgatca cgaaaatgcc gtagacggac accaccacgt
    16501 ggtcgatctg tgtcagacgg ctgccgcagg gaagcagcag gtctgacagc acgatgtact
    16561 gtcgggaatc cagccgccgc agccggcggg cgacctgttt ttcgccctgt atgccttttt
    16621 tgccggacgc tgccagccac ttcatgttct tccctccggt ctatgcaaaa aacggcaaag
    16681 cagtgatgct ctgccgctgc cgttattctc ccgcgttttc tgccggctgt gtcgaagcgg
    16741 ctctgccgga agaagcgtcc accagaatcg aggaatccga ggaaaccaca ttggggtatt
    16801 cgccgttcca cttttcgatc tgctggtact tcagaacgtt ttcgttcagg gacgcgttga
    16861 tcttttcgtt tgcctcggct tctgcctctg ccttttgccg aatggcatct gcctccgcct
    16921 tggcggcgat caccttttgt tcggcatctg cctgtgcttc aataactttc tgttcctttt
    16981 cggtttctgc ccgcagcttg ttctgctcgg caacctgttt ctgttcgatc gctgcgttga
    17041 actcctcgga gaagtcaaag ttgatgatat tnnnnnnnnn natgatattg aacttgtcga
    17101 tgagaatgcc gtattcctgc accttttccg ccagtgtggc accgatttcc tcgcctacgg
    17161 cggaacgctt ggtaatcagt tcttccgcgg tatactgggc ggtaatggct ttcatggatt
    17221 cctgaattgc cggctggagc acggtgtcgg catagccctc gccgatgctc tgatagatct
    17281 tggcagaatc gtccttgttg atgtggtagt tgatcgccac gccgctggaa acggtttgca
    17341 gatccttgga aacaccgttg gaatcgatct cggtcttttg gatcttgttg ttcatcatca
    17401 cgatctgctg ggcaaagggc actttcagat gcatgccctc gctcagtacc cgatcagaaa
    17461 cctttcccat ggtgacgatc acgccggtgt gtcccgccgg aacgatggtc acagcagacg
    17521 tgccggcaat cagagccaga agcactaccg caccaatgag gatcccgttc cgcagtttgt
    17581 tgtttttcat agacattcct ttcctgaaca attgaaatgt tcgtttttct ttgggtgttt
    17641 ccttaattat agcataatct cgctcttttt gtcaagatat gcacgttccg gtgccacgga
    17701 aatcaatttt catgatagcc tcccctgaaa ggggtgactc cccacgggg
//
LOCUS       DAWBGR010000031        17690 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1081_length_17690_cov_5.220471, whole genome shotgun sequence.
ACCESSION   DAWBGR010000031 DAWBGR010000000
VERSION     DAWBGR010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17690)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17690)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17690
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1081_length_17690_cov_5.220471"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3626 a         5174 c         4699 g         4191 t
ORIGIN      
        1 gcagccctag aaagtatact tcgcgggtga acccctccac cagcttgcaa acgccgtaaa
       61 aaataagatt caccgtctgc acaggaaaac acctgaatat cagcattacc gatacaggca
      121 agggcaacaa ccggcgttgg caagccacag gcggacagcg agtgtaacga gcgaattccg
      181 ccggacggtt ttgacatggc tgcactagaa gttacgctcc cctgcctctt tcaagggcga
      241 ttatgcacaa accgatacga ttcccgcgag gcgatatggg catcgccctc tacatattca
      301 aaaaagcaag gaaattgcga cgttttttca gaaagccagc ttctcacaat tgcagtggga
      361 ttccgctcag tggggtttct gcacacgcct gttctgcgac ttccagtgat gcatatccgt
      421 atgctctgac ggtttccgcc atggtcatgc acgccagatt ttttccagcc tgatacagcg
      481 acaccaccca acggccatag ccatagagca cttcaaaagg atacaccgaa agttccccgc
      541 ggagataggt ttcgtaggaa gtctgtgccg tggaatcctc cgccgtatga atcacacggg
      601 cacggctgcc cagatgcgga tactttgccg cgaattcctc catccacgga atctgaatgg
      661 caatgactgc ctcccgcagc tgcacaaatt ccggcgacag ttccggcagc tggtcggcaa
      721 agccggcgta cagctgaggg tctgtgctct ccatcatgcg ggcgtacttt tccataatgg
      781 ggttccgccc cgcctgcact gccgcggtaa acgccgccag ccattgttcc agcagctcct
      841 cctcccacgg cagatactgg ctccgccgca tgatcgaaaa ggtttcccag tcgtcctgac
      901 aggctgccct gccgccgatg ccctgcactt tatcgaacgt gcggaattcc agttccgcca
      961 gctgttccac cgtggcacgg tgcttttcca gagccgccgc ctgtgccgcc aacgtcttgg
     1021 cggtatccgc caaaaacgga gcctgtgccg tggttttttc ggttttgtcc attgctgtgc
     1081 actcctttcc gattgccgca caaatgctct cgatctgtcc gctgccgtcc tgctccggcg
     1141 gcaaaagcag caggtttcgc aaggattccg ccagtgctcc gaagccctcc agttccgcgg
     1201 tgctccgcag gagccacttc tcatagggag cgtaccggtt ccggagcaga tgacacgctt
     1261 ccatggcatt tttgcagaat tctgtcaaat acagctgtgc tgtggcgtag tcgccccgct
     1321 gccgcatgcg ggggtagttg tactgtccgc actgtgccat gcgtcccagt gcctgtgcca
     1381 gccgccgcag ccgcacttcc tccggatagc ccagcgacag ctttcgccgc accgccgtga
     1441 agctgccgga atcgtcccga aaaactgccc cactgcacgc cgccgccagc atgccggact
     1501 cgatttccag ccactgggct tctgtttccg gtacgccgtc tgttcccagc agccgccgga
     1561 aaaaggcact ctgccggcac acgcccactc tgccgtctgc ctccggcatg gcagcacggg
     1621 acacgccgca gtagcttttc ggcaaaacgg cataagcgtt ccgcagctgc tgcactgttt
     1681 cttccggcag atcgtccggc acccagatgc agaaaccggc tccgaaatcg tggtcgcggg
     1741 acagggcatc atcatagccc agacactccg aaccctcccc cgcaagaccg atcgccagcc
     1801 gcggcaagac cgccccgaag ttccgttcca gcatcgattt tccgaaagca atatagtacc
     1861 gttcgcagag cctcaatccc cgctcctccg gccgactgcc gcccaatcgg gcataagtct
     1921 gcttcagatt ttccgacacg atctcgtaga aattgttccg ccccatgtgc agctcgatct
     1981 ccggcagggc tttttcgtag gcatcggcag ccttggcgta ttcccccagc tgatagtatg
     2041 cgtccccgaa gccggcatac acggcactgg cgtgaaaatc cgagggcgtt ttgccgatca
     2101 gaatcttttc cgcctgctgc aaatagcaaa gtgcttctgc cgtccggtga agccggagca
     2161 gagaaaccgc cagatttgcg gcagaaatgc cggtacgggt gggatcagag tcatgctgta
     2221 ccagttccag agcctgtttc agacaggtgc aagcctgctc ccactgctct gtttcctgat
     2281 agaggagagc cagattattc caaaggcttg ccacacgccc gtcgtccggc ggcagcaggc
     2341 gggcatagag ggactgcacg gtttcgtagg cggcaaaagc gtcctcatag tttccggcag
     2401 cccgataggc attggcacag ttgagcaagg tcgtggcgta ggaaatggta tcggtttcac
     2461 cggtctgctc gaacaatgcc cgtaccgcgg caacagtttc cagagccgcg ggaaacttcc
     2521 cgcagtcccg atagaagccg atctgttcgt tccgcagcag cttttcgctg tcggtgtctg
     2581 cctccgcctg tgcctgtgca acggcttggg tgagcagggt ttctgcttcg tccagccggc
     2641 actggtgcag caggtcgtcc agacgggaga gagtttgctg tatctgcata gagttacgct
     2701 tcttccggca gcgtggtgaa gaaatgctgt gccagataga atgtagcacc ggtggacagt
     2761 tcgctgaagc cggtcttttc tgccggcata gccagattcg gtgcatcgat cattgcggtc
     2821 atcggctccg gacagaagta gtgcttttcg ccgcggtcgt tccacagaat ccagaagtga
     2881 tagccgtcgg aaacctcata gccgataccc ttgccgctgg cggtgtcggt catcagcact
     2941 ccgcggaacg gcttaccccg ataggtcagc ttgccggcgg cgtacatgtc gttgtccacg
     3001 atccgcttta ccggacaggt ggtgccgttc aggtatgcct tatcgtagtc gttcagcggc
     3061 aggatctctc cggtaggcag gcagcgttcg ttcagtgtcc acttttccac taccggcact
     3121 tgcaggcgaa tgtcttcttc tctggctccg tctacaaagg gggcagaaat ggtggtgtgg
     3181 gttgctacag aaaccggcag cttcttcggg gactggttgg taatggagaa cttgtactcc
     3241 agaccgtatt cactcaacac gaagaaaaag tccgctgtga accgcagggg catgtactgg
     3301 aagaacggat cctcctcgtt gtacacataa gaagtccgca gccatgcacc ttcctcattg
     3361 gcggaatatg ccacaacgtt atgctcccgc ttatgaagga aaccgtggat atggttgtgg
     3421 aacggagccg cttcgtttac cggcagctga taggccgcat cagaggtttt cagcacgccg
     3481 tctgcaaaac ggttcggcag gtacaaggtc ggcagacccc agacttccgc agactgcttg
     3541 atgtcctccg gtgtgttgtt ctcgtcccag cggaaaaatt ccacgccgtt ggcatcgtcg
     3601 tgaagccgca gcacgttcga accgattccc ggtgcgatca gtgcggtata gccgccggca
     3661 caaagctgaa tgcattccat acccttccag ttgatttttg ttgctgtgtt cttcataata
     3721 tgttcctttc cggaattccc gttcaccgga gaatcccagc gatattttgg gcattctgac
     3781 gaactgtgca gtccgtaacg cctatatgtt tgttattata gcatattttt ctcgttttgt
     3841 ctatagaagc agggaaaaat attgggattt tccaagcact cgctcttgac aaatctgccg
     3901 cggtgtagta taatgaaaat agaaaagagc ttatcaagca agcggtatgc tcccatagtt
     3961 tcaggtaaaa ataaccgtca caagtttggt agcaaggggc ggttattttt tcttattgtg
     4021 atgattactg tttttatgat tgtagtagat gtatgcgata aaggttgcca attcgaggag
     4081 aaaactcccc agtttcagca aatcgccaaa agttacaacg ctgtccatca taaagaatca
     4141 cctcccatct gttcaagagg aacctatggg agctaagaaa aggaatacca gcccgtcgcc
     4201 cagcggaaat ggcgacggca gactcgccca aggatagcga gccgcctacc gtgaatgata
     4261 agccctttcc tctagcataa cagaaaacga gcatgttgtc aaatcactcg tttttgtcca
     4321 aaacaaaaag cacattgccc tcggaaccgc tccgttggca atgtgctttt tctgtttcta
     4381 tctataatct tatttgtccc cgtcaatata cttccagcag cccttgagat agaccagacc
     4441 ggagataatg gtgagggcgg tggagatcca gatcagtgct cccagaatcc acgttttcac
     4501 gaagtccggc actgccagac cgaagtcata cgccagcgac aggtacgcca taacgcctac
     4561 aatggtcacc atggtgaatg cggtcttcag cttgccccag atgcctgcgg caatgacaac
     4621 gccttcctcc gcggccagca gccgcagacc ggaaaccatg aattcccgtg caatgatgat
     4681 gaccagcgga attgccgaca tgcggaattc tttcagttcc acaaacaccg tcagggcggt
     4741 gatgaccagc accttatccg ccagcgggtc gaggaacttt ccgaagttgg tcaccagccc
     4801 tctggcacgg gcaatcttac cgtccagcat atccgtcagc gaagccgccg cgaaaatgac
     4861 cagtgcccac agaaaatgca gcggaatcgc gtccacatac atcatcacca gaaaaaacgg
     4921 aatcaggatc acacgcagca gcgtcagttt attcggcagg ttcatcggtt acctcctccg
     4981 ccaacaggtc gtagtccata gtgtcgaaaa cttctaccgt gatgtactgt cccagacgca
     5041 gacggctgct gctcttgatg aacaccttgc cgtcaatatc cggtgcatcg gcagcagttc
     5101 tgccgaagta acagccggcg tacttgtcat atccctcaac caccgcagtc attcgcttgc
     5161 ccagctttgc cgcattcttg gcagcactga cttccgcctg ctgctccatg agaatatcgg
     5221 cacggtgggc acggacatct tcgtccacct gatcttcaaa ttcggcagcc ttggtgccct
     5281 cctcctcgga gtaggcgaag cagcccagat gatcgaactg catatcctgc acgaagtcgc
     5341 ccagccggtt gaactgctcc ttggtttctc cggggaagcc ggtgatgagc gtggtacgca
     5401 gggtgatctc cggcacccgt tcccgaatgt gggtgatgag gttccgcagg gtttcctcgt
     5461 tgccggggcg gtgcattctc cgcaggatct cctcatcgca gtgctggatc ggcagatcca
     5521 gatagggcac catatggggc tcctctgcca gcacgtccag cagttcctcg gtgatgcgtt
     5581 ccggatagca gtacagcacc cgcacccagc ggatcccgtc aatgtggcac agttcccgca
     5641 gcagttccgg cagcctggac ttgccgtaca gatcggcacc gtatcgggtg gtatcctgtg
     5701 caatgacgat cagttccgtc acatggtgct ctgccagcca gcgtgcctcg tccagcacgt
     5761 cctccatagg tacgctgcgg tacggtccgc gaatggcagg aatggcacaa taggtacagt
     5821 tgttggaaca gccctcggca attttcaggt atgcgaaaaa gggcagggtg gtaatgatcc
     5881 ggtcgccctt catttcggca tcgctctttt tgccgaactg cacgacacgc tctccagcca
     5941 gcacgtggtc gagaatgtcc acgatgtccc gattgttgcc gatgccaata acggcatctg
     6001 cctcggtgaa cagttccgcc gcttcttcct gatagcgttc tgtcaggcag ccggtcagaa
     6061 tgatcttttt cagggtgccg tcctttttca gggcacccag ttccagaatg gtttcgatcg
     6121 cttcttcttt ggcggactgg ataaatccgc aggtgtttac gattgcaacg tcagcttcac
     6181 cgggttctgt cacaagctga tagccgtggg cacgcagctt tgccagcata cgctccgaat
     6241 cgaccagatt cttggaacat cccagagaaa ccatgcctac acgaattggc atattttttc
     6301 ctccatatga tcgttgtgat ataatattac agcggacaag ccgcctgttc ccatcactgc
     6361 cccgcagcag ccacagtttc cgtggtagtt tccgtttccg gagccggtgt agcctgcggt
     6421 tccggcacgt ccggatccgg cggcagctta tagttggggc tggcggggtg catgttgaaa
     6481 acgatcatgc cgttctccat ttccacgccc cgataatacg tcagctcgct aagggtcacc
     6541 agctgatagc cctgctgcac cagcgacggc acgatctgtg ccactgccgc agccgtttcc
     6601 ccgtagatgt cgtgcatcag caccacatca ccgtcctcga tgttgttcag ggttgcacta
     6661 acagtgctgg cagtgttccg cgttttccag tccctggtgt ccaccgacca gaacgccagc
     6721 ggtttcccca cgtggcggcg aacgtcatcg ttgaagtccc cgtagggcgg acggagaaaa
     6781 tagggatacc gtccgatcgc ctgcaagatg cggtcgtctg cctgctggat ctcctgctgg
     6841 aacgtgttcg catctgcctt caccaaatcg gcatggtttt ccgtatggct gccgatctca
     6901 cagcccagcc ccacggcacg ctgcatcaca gcactggttt cgtcgttgat gttctgtccc
     6961 accacgaaaa agctggcgtg tgcctgatag gcttccagcg tgtccagaat gctgttggta
     7021 tactgccccg gaccgtcgtc aaaggtcagt gccaccatag gctttgaggg gtcgatattg
     7081 gcgttctcat cgataaacgg ctggcagagc cctgccgcag tagtggtcgt cactgtagta
     7141 gtcacagtag ttgttgtggt ttcggttgtc gctgccgttg tggtttcgac tgtagtggaa
     7201 acttccgccg ccgaagctgc cgccataacg gcttcctgtg tctgtttgcc gtcggaacgc
     7261 actgcatcga ttttggtgca ggctccaccc agcagcacca acgctgccag cacggcacat
     7321 ttcgcgatcc ttctttctgt ctgcataata cccctccaaa tctcctgctt gagatcctta
     7381 ttccacattt tccatttctt ccaaaaatgc ctgcaccgcg gcgttgatct cgctgaagcg
     7441 atacccctgc cgtaccagtg cagctttccc cttttcaatc gtccggcgat cgtcgggatc
     7501 cagaaaatac ttccggtact tccccgccac cagttcctcc agtattcccc ctgtcacctc
     7561 gtcctcatag ggctccagtg cctcggtgat gtgttcgtcc agcagtcccc gccggtacat
     7621 ctcctgccgt gcccgccgca gcccgaagtg tttcacttcc acatagtggt gtgccagctg
     7681 ttccgcatag gctcggtcat tgatctgccc gaacttcgcc agccggtcta ctgtggcata
     7741 gcagacggac tggggatatt tcggctccag cttccggaac atttcctgat agctgtagcc
     7801 ccgccggtcc agcagaaaac aggcatactg gtacgccttg cgggcgagtt ccgtagtctg
     7861 cacctgctcc cactgctgcg gtgtcagcgt catgcccttt tgcagaccgc accgctgcac
     7921 cacagaaacg tggagaaaat acagttcgcc ggagtcgtcc agttcgagtt tccaggtgtt
     7981 gcccttatga gcagccagtg tggtaatggt catgctgtgc cgtcacctta cgccggatcg
     8041 aattcttcga agtcgtcctc tgcactgatc tccagatttt cgccgtcctc cagcgagttg
     8101 aactcgccct cggcttccag cacttcgccg ccgtcctccg gtgcctcggt gttctctccg
     8161 gcagcctttg ccgcagcttc ttccagcttg gacttcttct ttttcttctt ggtcgatgcc
     8221 atgagttcgt ccttgtgttc caggatctgg gcttcgatct ccttcatcag ttcggggttg
     8281 tcctccagtg ttttcttggc gttttcccgt ccctgtccca gcttctgatc ctgatagctg
     8341 aaccacgcac cgcccttttt gacgatgccc agttccacag ccacgtcgat gacctcgccg
     8401 gtacgggaga tgccctgtcc gtagagaatg tcgaactcgg cttcgcggaa cggcggagcg
     8461 accttgttct tgttgatctt caccttggtg cggttgccga tctgactgct gccgtcctta
     8521 atgggttcgc cgcggcggac ttccatgcgg acagaagcgt agaatttcag ggcacggccg
     8581 ccgggagtgg tttccggatt gccgtacatc acgccgatct tttcccgcac ctgattgatg
     8641 aagatcgcca cgcacttgga cttggagatc acggcggtaa gcttccgcat tgcctgggac
     8701 atcagacgtg ccagctgtcc cacgtggctg tcacccattt cgccgtcgat ctccgccttg
     8761 gtggtaaggg ctgccacaga gtccacgacc agcacgtcaa tggctccgga gcgtaccagt
     8821 gcttcggcaa tttccagagc ctgttcaccg ctgtccggct gggaaaccag caggttgtcg
     8881 atgttcacgc ccagtgcttc ggcataggtg gggtcaaggg cgtgttccac gtcgataaac
     8941 gccacttcgc cgcccatttt ctgtgcctct gcgataatgt gcagggcaac ggtggtctta
     9001 ccggagcttt ccggtccgta cagttccacg atacggccgc ggggtacgcc gccgatgccc
     9061 agagccatgt ccagcatcat ggagccggtg ggaatggctt ccacattcag gttcgccttg
     9121 ttctccccca gccgcatgat cgcaccggta ccgtatactt ttgtgatatg tgcaatggca
     9181 ctctggagtg cctttttctt ttcttcctcg gtggtgggga tatccagtac cacctttttc
     9241 ttcagatctg cttttgccat aaattatcgt cctttatctg gtatagcgaa tgaattcgct
     9301 ccttacggtc aatcatgtta ccaattgccc cgccgcatat cccgaaattc ctaccgctgc
     9361 cccagcacga cgcgtccgat gcctgtgagg tcggggacgg tgtgtacctg ggcatagccg
     9421 ttttcctgta ggatcgcttc cacgtccggt gcctgtcctg ctcccacctc atacaccagc
     9481 cagccagccg gctggagtgc cggcttccac aggggagtca gctttcggta gaagtccagc
     9541 ccgtcggtgc cgccgaacag ggcggtttcc ggttcacggg cgacttccgg ctgaagctgc
     9601 tccatatctg ctgcggtgag atagggcggg ttacaggtga tgatgtggaa ctgccggtgc
     9661 agcagttccg ccggcgggtg cagcacatcc ccggcgatgc actccagctg gggagcgtgg
     9721 cgtttcaggt tctgccggag atagcccatt gcttccggcg aaagttccag cacggcaccg
     9781 gtgacaccgg acaggtgcac cgacagtgcc gacgggatac agccggagcc gctgcaaaga
     9841 tccagcaaac aggtggacgg ctcattcttt cgcagttcca gcaccgcttc caccaatgtt
     9901 tcggtatcct gccgcgggat cagcacaccc tctcccacct gaaactccag cccgaaaaat
     9961 tcccatgtgc cgcagagata ctgtagcggt tcatgggcac agcggcgtgc ggtcagttcc
    10021 gccagacgct gctgctgggc tccggtcagg ggctgctgcg ggagaggtgc cccatagggc
    10081 agttccagca cggtttccag cagaatgcgg ctttcgaact gcaccgaatc cagtcccgct
    10141 tctgccagtt tctgctgcac cgcctgcatg gcggcacggg gcgtatttac cacttgtcgt
    10201 cctccatatc ctccggaatg cagggcttca gtgcagcaat ggcacactcg atctgtgatg
    10261 cgtccggttc ccgcgtggtg atccgctgga tttgcagacc gggccacgac agggcacggg
    10321 tcagccagtt gttgcttttg ccggcgatac ggataaactc ataggagatc ccgaccacca
    10381 ccggcagcag cagaatgctg acgatcacac ggggcagcac ctgataaatg ggcacgatgc
    10441 acattaccag aatgctgacg atcagggtga tgaaaataaa actggttccg cagcgagggt
    10501 ggaaacgagt gtgctttttg acgttctcca cggtcagttc ctcgcccgct tcatagcagg
    10561 caatggtctt atgttctgcc ccgtggtatt cataggtacg gcgaatatct ttcatcagac
    10621 cggtacacgc catgtacccc aaaaagatcg cgatcttgat aatgccctcc agcagcgacc
    10681 gcacaaagcg gctgttgtcg ggaatggcgg tgtgggagat cagctgtccg atgaggaatt
    10741 tcggcaggaa cacaaacaga ccaatggcaa cggcaatgcc gacgatcatg ccaatggtca
    10801 taatgatgcc gaacagctta tcccccagct tttcgtccag ccatttttca aagcggttca
    10861 tttcttcggt gtcgtcttct tcgtcctccg gtacctgttt ttctgccgac ttagtcaggc
    10921 agcgatagcc gtctttcatg gacagaacga agttgaagct gccccgcaca aaaggacact
    10981 tccggtacca cggcatattt ttgccgttgt tctccgccca cgtttctacg tcgatctcgc
    11041 cgttgggcag gcgacacgcc attgcaccgg tgtctgcccc tttcatcatc acgccctcga
    11101 tcagtgcctg tccgccgatc ttggtcatgt gcttggggtt ttctctgggt tgttttgcca
    11161 tatgtatctg tttcctttct cattcaatcg agatcgtgtt tttcacaacg tcccagtctg
    11221 catcaacgtc cagcctgaca cccttatggc aacaccgtac aaagccgtca ggcgggcttt
    11281 gtgcggtgtt gccttgggta cttctgcatc tctgctccct tcgtgttatg tggtggcact
    11341 gctgcggcgt tgcggctcta ccggcagcgt aatggtcacg ttggttccca cgttcagttc
    11401 gctggtgatg tccagtctgc cgccgtgcat ggtgatgatc tcatctgcca cagcaagtcc
    11461 gatgccggag ccgcggcggg tgtggtttgc cttgaaaaac ttggtcttga cgcggggcag
    11521 gtcggcttcg ctgatgccca cgccgttgtc ggtgatcgtc acctgtatca tgttccccac
    11581 ttcctccgca ctaatggtaa tggtgctgcc ggaattggag tacttaatgg cgttgtcaat
    11641 gacgttgatg aacacctgcc ggatacggtt tttatccccg tagacaaagg gcagcatttc
    11701 cggttcctga tagatgatgc ggatattctc ccgctttgcc ttttcggtat agatgagcac
    11761 cgcgtccccc agttccgcca gcacgtccat ggtggcacac tggagcgaga accgtccgct
    11821 ttgcatgcgg gaaaaatcca gcagttcctc aaccatttcg gacagccgtt cggtttcgtt
    11881 cacgatgaca tgcacgccac gctgtacggt gtcggggtcg gcttccatgc acatggtttc
    11941 cgcccagccc ttgatcgccg tcagcggtgt ccgcagttca tgggacaccg aggagatgaa
    12001 ctcgttcttc atgttctccg tattggagag ttcgtccgcc atctggttaa tggacacgca
    12061 gaggtcgccg atctcgtcgc tgctgttctc ctgaatgcgg acagagaaat ctcccttggc
    12121 gtatttcttg gtcgtggcgt tgatctgctt aatggggcgg acaatggagc gtatgaaata
    12181 cagtccggtc accaccagca gcagcagcac gccggcacac aaggcactga caccccacac
    12241 ataggaattc actgtgctct ggatctgttc cagagaagcc accacccgaa tggcgttgta
    12301 ttccgtgttc atatcggaaa tatccacgga aaccgccata atggtttcac cgccctgctt
    12361 gccgacccag tagccgtcgc cgccgtccat gacggcttca taatccggca tggcggcacc
    12421 ctccgccggt gtgaagccgg aggaggtcag tgccacccgt ccgcgggaat tgatcgccat
    12481 gagttccatt ttgtccttgt cggaaaagtt ttcgatggta ctccgcattt ccgaggaaaa
    12541 gttgatgtcc gcgtcctgtg catagcggct caggacaccg gtcacggaac tgatcttcgt
    12601 gttcagatac tgctgagaag cactataata ataattctgc atgatgtaga taaacgcgaa
    12661 atccagcact gtcagcacca gtgcaatgat ccccagattg ttgatgaccc agcggcgggt
    12721 gatcgaactt ccggcacgtt ttttcgggct gcgtctgcgt ttatttgtca ttccacttat
    12781 atccataacc ccagatcgtc gtaatatact gcggcttgct gggttcttct tcaatcttca
    12841 tgcgaatgcg gcggatattg acatcgacaa ttttatcatc gccgaaataa ccgtcgcccc
    12901 agatatggtt cagaatgctg gcacgatcca gtgccgtatt ctggttattg agaaaatact
    12961 ccatgatctg gtattccacc tgtgtcaggt caatcagctt gccgtttttg gtgacggtgc
    13021 ggcttttcag attcagggta aacgggccgc tttcgatcac cggcgactgt tcgtccttga
    13081 taaagctcat gcagacacgg cgatagatcg cgtccactct ggctgtcagt tccgaggggg
    13141 aaaacggctt tgtgatatag tcgtccgccc cgaacatcag cccgctgacc ttatccattt
    13201 cctgtgttct ggcagtcagc ataatgatgc cgatggtaga actcttttcc cgcagctttt
    13261 tgcagacggt aaagccgtca atgccgggca tcataatatc cagcaggaca atgtcaaaat
    13321 tgccgtgttc ttcttcgaac acacgcagtg cgtcctcgcc gcagccgaca tcacaaacgt
    13381 catagccggc acgcttgagg ttaatgacga caaagtcccg aatcacatct tcatcttcac
    13441 acactaaaat tcgtttcatc tctacacctc tgtttgggta cacctctgtt tgggtttgat
    13501 ctggggaatg tatgggatcc tctgtgaaat ctgatccata ccaaaaacgc ccagccgtca
    13561 cgctcccgcc actcactgca cgaaccggaa ccgcaccata aggttctgcc acggctgtgc
    13621 cagcgggtcg tcggtttcgg cggctttcat ataataggaa gctttgccgc gggtgtggag
    13681 cagctgatag ccctccgtgt cccgttcttc ctgtgattcc gtatcctgtg ccacgctgta
    13741 ccgcagcagc tctgtcatat ggtcgttcat ctcgccgtca tagcggcaaa agacaatatc
    13801 tcccgtgagg gtatccgtca ctgccgtcac tttatcgtac cacgactgcg gcagcaggaa
    13861 catacagccg tcattcaggc tgaaataccc ccgcattttc tcttgcagct ggctgccgga
    13921 aacgcccagc cagcgtgtca gccgcacctg atcggaggaa tctgcaccat agccgggaaa
    13981 cggctcctgc accggaatct ccacgctgcc gtcctcgtcc aagtccatgg acagatagcc
    14041 tacagagcga ttggtctttg ccaccacatc gctgtcggcg agcacatacg ccagcgtttc
    14101 tccctgtacg tggaggactt ccgtttgcag cgaggaggga cctatggcac cgtcgatata
    14161 gatgccggtg gtgccgtcct ccagtctgcc gtactgcacc tgactgaaat ccgtaaagcc
    14221 agtccgcaga tccagcttgc cattgtcctt taccgtcccg ttttgcaggc ggtacattgc
    14281 cgcagaggag gaagtgtttt cggtgagttt ccccagcacc agcagttcct gtgcctgatc
    14341 gccgtccaga tctgccacgt caaacatggt atacgcggca gtgaacagct gctgcacttc
    14401 gctgtcgctg tagcggtaga cggtcaggga tttgtccgac tggtctacgc cgctgtagcc
    14461 cacgaagatc tgattctggg aatcttcgct gcccatggga gagatgacga tgcgttccac
    14521 ttccgtgccg tctgccggac ggtcgcagac cgacatccac tcccccttca cctgatccaa
    14581 tacgctgagc cgcagaccgt tttccatggc ggtaaggctg gttttcttat agaacaccaa
    14641 cgcttcgtcc tcgccgtcgt tgtccagatc gactaccgta aaggcagaca gattggctcc
    14701 ggctctgggg tattgcaggg tgatgccgct gcccactgcg tcctgtagtg ccagatagat
    14761 ctgctgctgt tccttgctca gactgggcgg tttcagcagg gaatccaccg tcgcccccgg
    14821 cgtacagccg gaaaacagca ggcagcaaag taggcaaaat gcggctggca gccaccgccg
    14881 ccgccgcatc atagaatgct ttaccgatcg catagcggaa cgtactcctt cgactttgcg
    14941 atcgccttat atgccggtcg gataattcgt tttccggtga gaagctcttc catacggtgt
    15001 gccgcccagc ccaccattct ggcggtggcg aacagcggcg tgaacaactc gtccggaatg
    15061 cccagcatgc gatacaccag accggagtac atgtccacat tggcacagac caccttgtca
    15121 ttgccgcgga ccgatttcat cagttccggt gtcagctttt caaccgtatc cagcagtctg
    15181 aactcccgtt cgatctcctt gcccgcagcc agagccattg ctttccgctt caggatcact
    15241 gcacgggggt cggacagcgt atataccgca tgtcccatac cgtagatcaa accggagccg
    15301 tcgttggctt ccttccggag gatcttggtg aggtagtctg ccacttcgcc ctcgttctcc
    15361 cagtgtgcca cgtgtgcctt gatgtcgtcc agcatctgaa tgaccttgat gttcgcaccg
    15421 ccgtggcggg gtcccttgag ggagttgatc gcggaggtat atgccgcaaa ggggtctgta
    15481 ccggaggacg ttgccacgcg gcaggcaaag gtggagttgt ttccgccgcc gtggtctgcg
    15541 tggagtgcca gcagggtatc cagcagctgt gcctctgcct tggtatagga acggtcgttc
    15601 cggatcaggg acagaatgga ttctgcggtg ttttcctccg gacgcagcgg gtgcataatc
    15661 aggctggcgt tgtcatactt gtgccgcttt gcctgatagg caccgtccat gagaatgggc
    15721 atcttggcga tcagatccag agcgatggac atttcatctt caatagtggt gttttctgtc
    15781 acgtccgaat aggaatacag ggacaggata cttctcgcca gcttgttcat gatgttgttg
    15841 gacggggact gcataatgga gtccaccaga aatcccggcg gcagttcccg ccgcacaccc
    15901 agcgaacggg tcatgcgttc cagatctgcc ccgttgggga gcctgccgaa cagcagcagg
    15961 tatgcgactt cctcaaacac atagcggtct tcccgttctg cattttcaat gaggtcgtta
    16021 atatcataac cgcggtagat gagctggccg gggatcggct ggcgttcgcc ctcgctcatc
    16081 acgtagccgt gtaccataca gatacgggta atcccagcaa tgacgccgga accgtctgca
    16141 tttctcaagc cgcgtttcac atcgaagcgt tcataatatt tcggagcaat atcggaatat
    16201 tgcaccaggt cttcacataa attatgaata tcagaatcct cgatgctgtg gttgtaaatc
    16261 agttgcggtg tcatagagat tgcctccttt ttaatcgtta ccttatatta tacacaaaaa
    16321 accgcctcct gtcaatcgtt ttgcggtttt tttgtgcggc agaaaaattg gaacaaaatc
    16381 ccaattcacc agaaaacaaa agaaaggaaa gcggatcgca ccaatgaaaa aatatttgat
    16441 gattttaggc ggcttcaccg gtatattatt tctttttacc gtgatcccgt cccgcttcgc
    16501 cgcccctgcc tccgatgcgg cagagccgcc ggtcgccacg gaaactgcca cgatccccgc
    16561 agaaaccgcc acttccacca cggcagaacc tgccggacgg gctgacctgt gtgaaaacta
    16621 tcagatgctg gacatcacca gcggcaaggt ggaggaactc tccgtgcggg attatgtgat
    16681 cggggcagtg tgtgcagaga tgcctgccac ctttgaacca gaggcactga aagcacaggc
    16741 tgttgcggca catacatatg cggaacggca gcatttgctg gaacagtccc accccaccgc
    16801 ggaactgtgc ggggcggatt tttccaacga cagcagccag tatcaggcgt gcttcacgga
    16861 aaatcaggca cggcagtact acgaggacaa cttcgaagct tcctacgcca agatcaccgc
    16921 cgccgtggac gaggtgctgc cctatgtact ggaatacgac ggcacgccga tcattgcggc
    16981 gttctgctcc atgtccgccg gcaccacgga aagtgcggaa accgtctggg gcagcaaggt
    17041 ggaatacctt gttccagtgg agagcgaagc agacacctct gccccgcggt atctggaaac
    17101 cgcatcgttc accggcgagg aatttcaggc ggctgtgcag gacgcactgc ccgatgcagt
    17161 ctgggacacg gatatggcga aatgggtgca ggtaggagcg tgttcccagt cggggacggt
    17221 tctggaagtc actgtgggcg gaaaaacctg ctccggtcag gaactgcggc agatgctggg
    17281 gctgcggtcg gcggtgttct ccgtggacgt gcaggacggc aagctggtat tcaccaccaa
    17341 gggctacggg cacggcgtgg gcatgagcca gtatggagcc aatgctatgg caaaagacgg
    17401 agcagactgg caggaaattc tatgtcacta ctaccctgcc gcacagctgg tgcagggaga
    17461 actgccggcg taatttctac agatgataca atgttttcaa catagttttc aacattttaa
    17521 catacatcaa caaacatcat ttgagctggt tcgccagcag tcgtggtttt tcttgggcaa
    17581 taccgcacaa agattgtgcg gtattgcctt tgtacgattg cggaaacact gcataaagga
    17641 aacactgaat aaagcttgtg cgtaagatgc gtagtcagat ttttcggcag
//
LOCUS       DAWBGR010000032        17528 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1096_length_17528_cov_3.523493, whole genome shotgun sequence.
ACCESSION   DAWBGR010000032 DAWBGR010000000
VERSION     DAWBGR010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17528)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17528)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17528
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1096_length_17528_cov_3.523493"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9855..9864
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14964..14973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16509..16518
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17250..17259
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4556 a         4567 c         3956 g         4409 t
ORIGIN      
        1 tttcatctga cgaaaaattt gacgacgcat ctgtcgcaca atctttgtgc ggtagtgccc
       61 ttgattcagt ggttttcacg attcggttcg atcaaggcaa actggctatg ctggttcagg
      121 tccactacgg catcgtgaat ctgctgcaaa tattccaaat gctcggtttc cacaaacggc
      181 agcagtgttt ccaagttctt cgccgtaatt gccggaaaag cagctgtggc aattcggatc
      241 agcggcagcg attcctccgt atgtccctcg taatagagaa tcgttccgtg ataaacgtgc
      301 acggcaaatt ccgcctgcac ctgaaatgcc tcgcctgcct gccatactgc cggctctccg
      361 gcggcatttg tcaccagctg taaccggaac tggaaatctg cgtctgccag cttttcgccg
      421 tctgtcacaa tgcccagata gtccagcacc attcgcggtg tcatgttctg tatcaggtca
      481 ttcttgccgt cctgctccag ccgccgcaca gtttccgaag tgccgttccg cagttcaaag
      541 gcaccgttta gatacgcatt ccgccagatg ccggattccg actggtacgc cagctgttcc
      601 agagcgtctg cgttcagcag ccgtgcccgc tggtttgacg gttctgcaaa taccgcatag
      661 gaagctgcct ttgcggcacg gcgataattg ccggcggcat agtcctgctc cgccagatgc
      721 agcacctgct ccacagaacc cacatattcc acaaacagct tggcttcctc tgtttctgtc
      781 agagcgttca gttctgtggg gttgccgttg taaaagccca gatatttgca gtagactgcc
      841 cgtgcgttga tctccacaga accgtaatac gggcgggtgt accagacttt cgccagcttc
      901 tccggcagta ccacttcccg tgcgatctcc ttggcagttc tgccctgatt tgccagatgc
      961 agcgtccggt cgtggatata tttgtacact gccgcattgt tccggagaaa atcctctacg
     1021 ccatgaggcg tttccggtgt gttccagtgc ggccagttgt gggactggaa caccacatct
     1081 gcctgcttgc cgtagcgttc cagtgcaatt gccgtaaacc gccaccaatt cgccgcatca
     1141 cgcagctgtg ctccccgaat gggatagaga ttgtggaggg ttccggtgca gttctccgcc
     1201 gcccagaatg cccgatattc cggaaaatag ttgttcatct ccgccggtgc ttctgtgccg
     1261 ggtgtcagct ggaattctac ctccagcccg tcaatggtca gcgtggtatc ctccgcaata
     1321 aaatcggtag gcgtgatata gctgagcgtt ccggaagaag tccccagtcc cagccctgtg
     1381 gaaactctgc cctttggacc ggcgagcaag tcgccgccga actggtactt ggaacggtgg
     1441 aacatcgctg ttccggcgta aacgttttcc ttgacagttt cttcgtcaaa gccagccggc
     1501 acgtaaatgc gtacctgtcc ctctgcctta cccacctgct gcggggaaac gatgcccttg
     1561 atgccgccga aatggtcggc gtgggagtgg ctgataatca cggctctgat gtggtcgcga
     1621 atgttctctc ccagcacttc ctcagtgata cgcaggccat aggcagccgc ttctgtattg
     1681 gtggtcacgt caatgacgat ccagccggtt ttactgcgga caaaggtcag gtttgccaca
     1741 tcaatgcccc gcacctgata gatcttccct gccagtactt caaagacacc ggcttcgata
     1801 ttcgccttgc cgttgagcca caggctgggg tgaacggtat ccgccactgc ctgctgtttc
     1861 agaaagtcgt atgcctgcaa gtcccagact acgtttccgg tcttgtcttt cagccggaat
     1921 ccgtccagct ttttcaggca gccgcgtcgt gccagtgcta cttcactgtt gtaagcatct
     1981 tccagatcaa ttgcaaactg ctgggcgaat gcggcgttat gctgcttggt aaagacagtt
     2041 gcttctttcg catctgcgtt ataactgtat tcagacattg ctgtttcctc cctttaaatt
     2101 cgttccagaa ccggcggttc ccattcgccc cgaatggtca gttcgtccgg tgtgtacacc
     2161 cgcaatgcca gtgtgaaatg ttcctccgca gcgggtgccg gcagccagtt tttccgtgcc
     2221 agtccgccct ccggttcctc tgaggcaatg aagatgtcca gcgaaccgtc cggattcttt
     2281 tctacgctgc cgttttccag atcataggta ttgaccttgt accgcccaat gggattggac
     2341 accagatact tgctgggctc gccgtacagc gtgatcgacc agaacacgga agcatgcgga
     2401 tagccgtccg ccggaatgtg cagccggtag cgattccggc tcgccaacgg tgcaccgctg
     2461 tcatctgccg ctgcaatgag ataggcactg tcctgaatga tgttggcacc ccagccgcca
     2521 taggcagttt ccgcacgctg gagatagtcg gtaccataag ttcctacgcc gccaatgaca
     2581 gagagccagc cgcggtgctg tacggtgtat ttcggattcc gttttcccgc ctgaatttct
     2641 gcaaaaccca gacggctgcc ctcggtcagt gcctgctgct gttccgcagt caggtcgtcc
     2701 catgaaaagg ctcctgtggt gcggtcatag ccgaactgtt caaaaatctg cacataggct
     2761 tcgttccgaa taggattttc cgcggaaagt gccgcaaaca gcgtgaagaa ttccttgggc
     2821 gaaacggttt ccacataggc tgccggagaa agctccggtg cctgccgaac cggacgcacc
     2881 cgttccggat acagcggacg aatggtaata gagtcttgga tttggttcac taccggataa
     2941 tctgcctcgc cccgcgtttc aatccgcagc agcactgcat tcagggaatc ctgtaagcga
     3001 atcacccgat aggattcgta tccagccggc accggtgtgt cctgatacag cagaatataa
     3061 tccccttctg ccctgtccgg agtccgtgtc ccgatggatt ccaccacatt ggtgtatgcg
     3121 tccagaatgg ggaacaaata gtagcgttcc gtgatcttcg gaatgtgtac cagatatggt
     3181 gtccgtgcca gctgtgtcca cgcggtggag tacagcgtgt cattgttcat tttcacgatc
     3241 ttgttcacct tgtcggtggg aaatgtccgc agatgctgaa atgtcttgtc gtctgctccc
     3301 cagtgggtcg cttcggtcac caccagcggg aagccataga gatagagtgc ctttgcctgt
     3361 tctttcaaat ttgccatttt ctgttcctcc ttattgttca cttgttttca gtttttcgta
     3421 ataagatgtg tccacaaagc tgtccacatc gattgccttg gagatgtagc cgttttccag
     3481 catgaagtcg ctgaggtagt tcagcttgtc ggtcacgccg ctgtcaatat cgctgtccag
     3541 aaaagcaaag gtatcatcga acccataggc tttccggaac acgtccacgc ccagtgtggg
     3601 ggatgcaatg gcgtgaaaga attcgtccgg attggaaacg gtttcctcct tggcacgctg
     3661 caacgccttt agtacagcca cagctgcgtc cggattttcc tgtaagaagt cgtttctgcc
     3721 ggtaacagtc caaaggcttg ccatttccgg atgtgccaca gagttttcca catcgacacc
     3781 caagccctga ttctcgtagt acactacatt atagtacaag gtcagccatg catctgcttc
     3841 gccggtggta aagacagatg cagcatcaga aaccacgttg acgatctcca ccttgctctc
     3901 atcaatgtca taggtgtcta cgagatgctt ccagtattcc tgatagtttg tgccgatacc
     3961 gataatgact tttttgccct ccagatcttt cggtgactgg attccggcat ctttctgtgc
     4021 cagcacacca gcgttcatct gcttatctgc cacgccgata acacgaatat ccacgccgtt
     4081 gctgtaagct gtcgccgccg gataatcccc gtagaatgcc atgtccagtt caccggaagc
     4141 atatgcctca ttgattgccg gacctgcctc ttggaacgtc accagatcca gcgtgtagcc
     4201 tgcggcattg agttcttctt ccagatagcc cttgtcgtag gcaagcttgc cgttttccag
     4261 aagcggatag tcgtcaaaac cagggcagcc cagccgcagg ggcttcagat caccagaagc
     4321 cgaagatgca gcctgctctg tgctgctgct gtcagaagaa ccggcactgc cgcagcccac
     4381 aataccaagg ctcaggacac ctgccagaag aaatgcgaaa atcttgttgt gtttcatttg
     4441 aaatacctcc attttttagg gcaacaccgc acaaagcctg tgcgtgactg ccattatttt
     4501 tgttttctat tccaaggcgt aatcactgta aagattccgc ccagaatctt atccatgaga
     4561 atcccgatca gtccgatgag aatcatgcac acgatcacca cgtccgactg catcaggctt
     4621 ctggcattgc tcatgcggta gccgataccg gacagagaag caatcagttc cgcagcgaca
     4681 actgccatcc acgaaatccc cagccccagt ttcagcccta ccagaatctg cggcagtgcg
     4741 tggggcagat ggaccttccg gaacgttttg aaaaagttca gcttgtacag ctgtgccacc
     4801 tcaatatagc ccgccggtgt actggaaatg ccgctcatgg tgttcatcat aatggggaag
     4861 aatgccgcca tgacgatgac taccactttg gacaattccc cgatgccgca ccacagaata
     4921 atcagcggaa tccatgcaat aatgggaatc tgccgaatca cagtcaccgt gggctgcaac
     4981 aggtgtaaca ccgttttgga catgcccatc agcgacccca gaaccgttcc caacacggcg
     5041 gaaagcagat accctttcag cacgcgggaa agtgtgactg aaaggtcatt ccagagctgc
     5101 ccgctttgca gcatctccag aaatgcctgc cacaccatgg aaattttcgg caggatactg
     5161 gtgggaatgt tcccgaaatg ggtagcacag atccacgccg ccagtatcag caccggaaca
     5221 atcagtccca gtaaaatctg tttgaccttc tgcgttttca tggtatctcc tccctcagat
     5281 catgtactct gcctcttgca gaaattccgc agaatcctga aagaagtgct cgtagatttt
     5341 ctttttgtat gcggcaaagc cggaactgcc gcggttcatg cgggagtatt tatcagtagt
     5401 gatgatctcc tgaatttcgc ccggccgtgc agacatgatt acaatgcgtt cgcccagata
     5461 gattgcttcg tcaatatcgt gggtgaccat gaccatggtg actttttcct cccgctgaat
     5521 ccgcagaatc tctttctgca tctggattcg tgtcagtgcg tccagtgcac cgaacggttc
     5581 gtccagcagc agaatggaag gattgccggc aagtccgcgg gcaattgcca cacgctggga
     5641 catgccgccg gacagctgcg agggataggc attttcaaag cctttcagtc ccaccagttc
     5701 cagatgccgt gcaatgtgct cttcccgttc tgccttagtc atattccgga taccgaagcc
     5761 aatgttgtcc ttgatcttca gccacggcag cagccggtga tcctgaaata ccataccgca
     5821 ctgtgcatct ggtccggaaa cggcgtgacc gtttcgggaa acgccgccgt cctgatacga
     5881 taccagaccg cagatgattt tcaaaagggt actctttccg cagccgctgt gtcccacaat
     5941 tgtgacaaac tctccttttt ttacggaaag atcaatgtct ttcaatacct gaaacgaagt
     6001 gccgtccttg acgaattctt tgttcagatg tgaaactgta atgatgtttt cctgttctga
     6061 ttttttcatt gctatcacct ttttccgatt tgccttaggg aattcattcc cttggtgtga
     6121 tttcattcta ccccaccgca agcgaaaagt ccagagaaaa tggtgacagc gttgtcattt
     6181 cacgtgattt tctccgccaa acgggtaaaa aaatgccgct tttctgtgaa aacagacagt
     6241 agctttcttt ttttcacagt acaagcggca acgatgccaa aaaagacttg acgaaaatac
     6301 gtggtttttg tttattttcc tacatttcct acaggatacc cagtatttca ttttagacaa
     6361 cgttatcata cgccttgaca agcagaagtt ttcccgctat aataaaatat agaatacaga
     6421 tagagattct cggatatgag gtgaggggaa atgtccatta caaaaattgc ggcaatcaca
     6481 ggggcatcgg tttccacggt agcacgggtg ctgcgggatc cggagcacaa gtgccactct
     6541 atggaactga aagagaagat cctacagact gcacgggaaa tcggctatgt gcccaacgaa
     6601 gcagcaaagg ctctgaaaag cggcagtgct ccgaacaaga tatttcatat caacatcttg
     6661 ctgacccgcg tcaactccga tgaaactgat ccgttctaca gcgaaatgct ccggctggtg
     6721 gagatcgaag tgcggaaaag cggcggactg attgaaaata tctggcggca cgcggtcttt
     6781 tccagcgaaa aaaagaccga cttttcagaa atcgaaaagt cggcggcaca aatgtttcat
     6841 gataccaagc aaaagcacga cgggctgatt ctcattggaa aatgctcgca aaaaggactg
     6901 aaatcgctga aacgctatga gaaaaatctc atttctatca accgcaactc caccacttat
     6961 gaagtggacg aggtcttgtg cgacggcaga aaaatcgcac tgacagcagt gaattatctg
     7021 atttcctgcg ggcatcaaaa aatcggctac gtaggagact gccataatga aacgcggtat
     7081 gtgggctatc aggaagcact ggtgcatcac ggcattgcac cggatattga cgatgtttac
     7141 gacatcacac ccaacgagaa aaacggctat caggccatgg catatttctc ccagcaggaa
     7201 acgccgccca gtgcgtttta ctgtgccaat gacattctgg cgatcggtat gctgaaatat
     7261 ctggcacaga gcaagaactg gtactatcac cccgcagtga tctccagcga caacattgtg
     7321 gaatctcagt acaccacgcc gatgctgaca acggtttccc tgccgaaaac ggaaatggca
     7381 catctggcga ttcagattct gacagaccgc atgaacggcg gtcacaaggc agtggtgaaa
     7441 acggaactgc aatccacact ggtaattcgc cagagcgtac atcagtatgt ttcaacagaa
     7501 aatgaaccgg aatattatat ttaaggcaat gccgcacaaa gtccgccaac gactctgtgc
     7561 ggggttttct tgattattcg gcatttccct ttgtgcggta ctgcgtaggt gtcatgccgg
     7621 tttccttttt gaactgctgc atgaagtaga tctcgctgtg atagccgcac tgttcggcga
     7681 tctccgcaag ggtattctgc ccgtctgcca gcagtttttt cgccatggcg attcgggcag
     7741 cgatcacatc ggacatggca ctgacaccga aattctgctt atacagccgc tgaaaatagg
     7801 cacggctcaa cagcatcatc tgtgccatat ctgcgatgtt ccagttttcc tggggacggc
     7861 tgtagatctt ttgccggagc gtgttcatgt tcttccaata ggcaagctgc ttttcctcct
     7921 ccagctgttt ctgtgcaatg acatggtcca cataggcgat cagttccgca agggataccg
     7981 tctggctgac tttttcacag aagcaactca tcagcagctt aacatgcaca ccggattccc
     8041 tttcttccag atgctgattg cagtactgct gaatggcatc gatatgcgtt ctgccagcat
     8101 caaaccggta agaataggaa ccgatcagat aaaagtggtc gccgttgcag cggcagcaaa
     8161 tttcgtttcc caagcagctg ctgctgaggg catctgccac ggtcatcaga attttatccc
     8221 ccacgttgct gccctgtttc cggttgatct cgtgcagatt gtaaatatcc actgccacca
     8281 gcaggaactt ctccccgtgc agaatggact gctcgtaaat ttcagaggac aactcctcga
     8341 agccgcggcg gttatagatt cccgtaagcg ggtcgcgaat ggcagtgagg tggatccgcc
     8401 cgctgaactg tttcagatag tttcggatac gcatggtttc cagagccgag ctgacatatt
     8461 ccgtccacgt caggaactgc ttgtccaagc tgtactgttc tccctcgaac tccaccgcca
     8521 gaaatccaaa gcagcggtcc tcatagtgca gcggaaccag atgataggca gagggcgttt
     8581 ccggattctg caaaatttcc accaattccc gcaggtggac gatctcataa tcggaactgc
     8641 cgtacacctg ccggaacaga aaaaatgtgt ccggataccc tgcggtgcgg tatgtgctgc
     8701 cattggtgct gatgatgccg tcccagtctt cgccgataca gaggtagaac accttgatgc
     8761 ggcgaatcag gtatagcacg tggtgcagtt ctttcgcaaa ttccatcaag ttgtccggtg
     8821 cggaaatttt ctgaatcatg ccgctgtact gaagcaaata agcaaacttc agctgctctg
     8881 ccagatctcg cttgtatccg gcaaacacac catttccgga catggtacag ccgcagcttt
     8941 ccgcaggctc cagcacttcc ggtcggcatg gaatgggagt acagatttca ccggtgattt
     9001 cgtagtgcag ctgacaaacg ctgttgactc ccagattata gttcatgctt gtcatacaag
     9061 tgattgaagg aacgttgagc atattggtca taaacgggtc ataccccacg actgcaatat
     9121 cttccggaac ggcaatgccg cgtttttgca gcgtgcggat cagagcctct gccattgccg
     9181 cattggcaca tgccaccgct tccggcattg caacaccgcc gtctgcaatg gcattcccca
     9241 gccgttcgcc ggagtgcacc cagaaatccc cgtaaaatat catctgttcc gacaccggaa
     9301 gcccgtgctg ctgcattgca tcccgaaatc ccaggacacg gttgcgggac tggtgcagtt
     9361 ccggcggacc agtcaggcag tagatcttac gcttgtggtg cacttcgatc aaatggttca
     9421 ccagatcgca gaaaatcttc cggtcattcc aaagcggata cactgcgtca caattttcct
     9481 cgccgatgtc cagaaacggc aaaccggatt ccagacagat ctggtcgatt cgctgccgca
     9541 cctccggctt ctgaaacatg cccttttgtg tgagaatacc gtcggcatac cctttgtgaa
     9601 acaatattct cctcccctgt ctgaaacggc gtgtcacagt tggcattgga aaaaaaggaa
     9661 aatgtgacaa tatcatagcg gagagcattc gcctgcttgg tgatgccctc taacaatgtg
     9721 tgattgtact ccgattgcag atctgtcagg aacactgcca gaatgccgcg ttttttcggg
     9781 aatgcctgca tgatttcacc gccttttcct atggcaacac cgtacaaaac ttgtgtaata
     9841 ttacttttag gcaannnnnn nnnncgtatg cagattgcgt gcaaagtcgc cagacgctct
     9901 ttgtgcggta ttgcctttat tatagcggaa aaagcggctg atgtcaataa gaattataag
     9961 atattattct cactgcaatg aaataatagc acaatttctt ataaaatagc atcatatctg
    10021 attgaattat ctcgtgaact gtgataaaat atagctacaa tccacaatac caaaacagga
    10081 ggaattggga atgaaacaac aaagaaagaa caaacacctg ctgcccgcca tactgaccgt
    10141 actggcaatt ctgagcagtc tgcttggaat tctgccggca ggttctgtat cggctgcgga
    10201 cgtgaccgct tatccggcac aggcagtaca tttcggggcg tacaccacga accggaatct
    10261 gaacaatgcc ggcagtgccg ccaacacgca gaaagccgcc ggagccaatt cagaggactg
    10321 gcgtattgac tatgtttctg ccggcgtgta ccaaattgtc agtctgactg acggcaagta
    10381 tctgactgcc aacggcacag catgcacact gacggcaaag gctgctgaca gcagccagaa
    10441 ctggaacatc gaaagcgtac agaaagactt cgagggatac gatctgtact acaagatcac
    10501 cagcgtttcc accggtgcgg cactgaccta ttatcaggga aataacacca tcgggctgac
    10561 cgcatacacc ggtgacggtg cccagaagtg gaagctgaat tgcagcggac tggaggggta
    10621 cgccgcaaat gcactggcaa atggcaagga aaaagcggga accattggcg gtctgctggg
    10681 cgaaaccgtt ttcgtttcca cagcggacga cttggaaaaa cagctgaata ccacagagcc
    10741 gaaaaccatt gtcatcacgg cggatattga tatgcagaac aagtcccaca cccgcattcg
    10801 ggacaacaag accattgtgg gttcttacgg gaacaagacc atttacgact ctcagttccg
    10861 caccaatgac acctacggtg cagtggacga caatcccagc gacaacatca ttttccggaa
    10921 tctgaacatg atcgcaaaga atgtgaagaa ccggatcttg atcaatatct ggtcctcgcg
    10981 tcagatctgg gtggatcact gcacgtttat ctcctacttg ccctcagatc atacgggaaa
    11041 cgggcaggac gaagtgggaa aattcatttg gctgaacacc ccctatgaaa gctatctgga
    11101 cgcaaaagac aacggcagaa gtccggacta tatcacgatt tcctataaca cattcaagaa
    11161 ccgtttctgg acggtggcat acggcacaca gaacagtgaa acttcccgct gccgcaccac
    11221 gctgatgtac aactggtggg acgaatgtgt ccgccgctgc ccgcagatcg gaaacggcag
    11281 cggtcacatc tacaacaact actattccgg cgatgacagc tttttgccga acagctgtaa
    11341 ccagatcatc agcggcgagg gctccaatat ggtcagcgaa aactgccgat tccaggcggt
    11401 ttccggacgg gaaatcattg tacagccgga tacttctccg tatcgggaca acggttccta
    11461 cacggcgaaa agcagttcag aaaccccgac caagctgaat tataccgcaa aagtcaccag
    11521 cacatggaac cccaaggaca actacggcta cacactgtta gatgcctaca acaccagggg
    11581 cacagacacc aaggatttct gcaccaaata cgccggtgct gcatcatctt ccggcgaact
    11641 gaaatacatc acagacagca gcctgcagac ctttgcggca actgtttaca cagcaccatt
    11701 cctgacggac agctttgatt ctgcctacgg caatccggtg actgactaca caccggcagt
    11761 tttgcaggaa ggtgcagtct acatgataca gaacgtcaac agcggactgt atctggaagt
    11821 ggacggcgga aaggctgcgg acggcaccaa cgtccagcag tggggtgcaa atgaacccgc
    11881 agcccacaat acatggcggg tactgtcaga cggtgacggc tattataccc tgtatcccca
    11941 gatcggtgac aaggtgagct atctgctgga cgttgccgga aatgctgcgg caaacggcac
    12001 caatctcggc atctggtctg ccaccgatgc cgatgcacag cggttcaaat tctatgaagc
    12061 cgcagacggt gtctattacc tgctcaccaa gaccagcggc gaccagagct gcgttgccgt
    12121 acaggcggct tccacaagtg ccggagccaa cgtggtggaa tgggcagtca acctctccga
    12181 cacttcccag cagtggaagc tgacacaggt agaggacacc ggctgtgtca tggataccac
    12241 aaagacatac cagttccgca atgccaacag caatctgtat ctggaagtgg caaacggaaa
    12301 ggctgaggac ggcagcaacg tgcagcagtg gggtgcaaat gaacccgcag cccacaactc
    12361 atggacactg aaagaattcg gcggcggata ctactacatt ctctctgctc tggcagacgg
    12421 caagacctac tatctgaatt ctgcgggaga agcagacggc tccaacatcg agatccacac
    12481 caacaacaag tccagctcgc acctgttcaa gtttgtcaag aatcccgacg gaacatatac
    12541 gatcctgaca cgcacttcaa aagatcgggc tgctgtagaa gtggcaaacg cggagaaatc
    12601 cagcggtgcc aatgtgcagc agtggacggt caacggcaat gcctgccaga agtggaatgc
    12661 ggtcgccttt accacgacag ctgcgacaac aacaactact actaccacaa caactactgc
    12721 cactgccgcg aaaacaacgg cagcaactgt tcctgctacg gccactgcca acacgtccaa
    12781 aaccactgca acttccgcac aggaacaaac tactgtgaca acagcgacta cactgcctgc
    12841 ggctacatct gcggctacag aaactgaaac agctgcatct acgcaagcta ccgtttccga
    12901 aaccaccgca gccacaacct ccctcacaga gcaggacacc acaactctgt cctctgcgga
    12961 aaccaccgcc gcaacttctg ccacaacggc aaccgaatcc tctggtacgt ccccgacaac
    13021 ggaaaccgtg atcttcggcg atgtcaatct ggacggaaag gtggacattt cagacgcggt
    13081 tctgttgaac aaaacggttg ccggaaccat cagcctgagc gaatctgccc aaaaaaatgc
    13141 cgactgcgac ggtgacggag aaaccggcag caatgatgca gttgtgctgc tgcggtttct
    13201 ggtgcacctg attcaaacgc tgcccagtac ggaatgacgc tgcataatgt ttgtgcggtg
    13261 ttaccctaag tctgaaaaac caatgcggga aaacgccttt tgaaaaagag ttttcccttt
    13321 gcattacaac caaaaagcac atcacgttcg ccagaccagc ggggtctgct gcgattcgcg
    13381 atgtgctttt tctgttttcg atatttcgtt tccggtttct ccgaaagcgt tttgggaaac
    13441 agcatcgttg acaatacttt cacaccttcg ttttcagccc tgtcggatac agcaatccgg
    13501 cagggctgaa caaaactcat tttttcattt aggcaatatt tcaaacgatc gttatttcac
    13561 cggcaggctg tccacagcgt gcaccaagaa ctgcatcaga gtagtgacat catcaatatc
    13621 gatctcgccg ctgccgtcgc agtccatgtt ctttttctgc atgtcattca cagtcacact
    13681 gccgttgacc gcctttgcca gaagaacaga atccaaaagg tttacctttc cgtccagatt
    13741 tacgtccccg caaatgccgg tatcgtcggg cagataattg cactttaaaa catcagcata
    13801 ggaacaaccg tcgtcctctg tagatgctat aattttttca tattctttcg caaatggact
    13861 attggggtag caatatattt gacgaatttc attaggagaa cgaagtgtta ttgacattac
    13921 aaatgtattt aaatccgtat gttcatagaa cttattttcc ggcggaatga aatctttttc
    13981 aaaaacaatt ttacggcaag gaagttgcgg catttcatct attcttatcg tagaaccgct
    14041 aatatgcaat tcgcgattag gatagtcata agtccatgtt gcaccagatg cacaggttcc
    14101 actggacgga tattttgaat cgtctgtagt ttccatgtac gatgttgccg ttgcgacatc
    14161 aaccgctgcc gccggcaaag caagacagtt cacactgcca atcaatgcgg aaaagagaag
    14221 tacgtttttc aaaatttttt tcatagaaaa catctccttt catgaaaaga aaattacagt
    14281 tctacttgtc ctgctgctac cttagagtac agtataacgt cctgatcgtc cagtcttccg
    14341 tcgcggttaa tatccgcaga gatttttgca agatcaatcg gtgccgtaac tccctgaaaa
    14401 ccaaagtcta aagcttcgtc tgcatcagca ggatttttct taagtcttgc catataagtt
    14461 gcaattgcat tccagtcgtt agaatctacg ccgccatctc tatccagatc tccaaataca
    14521 tatgtggcaa tgtcaatagt agaaccagat gaatccattg tatatgtata gttaattctt
    14581 cttctaagct ctgcttcgct tttcactctc ttagtgtagg ataaattagc tacaccgatt
    14641 actttagctt cactggacgg ataagctttc atatttgaaa acatagcatc atcaaatacc
    14701 acagtttcat catcatcgaa ataagcacct gatgtagaaa gacgaccacc atagtttaca
    14761 taagtgaacg gaatagtcat aaaattaata gaactgatat aagaggtgtc tgcattcaaa
    14821 taaacattaa cacgtcctgt tgtactttca gcgtttgtag taatcaaagc aacatagtgt
    14881 tcttcgttaa gcaaatattc attgtaatac gctctctttc cagaattgct tgcataagca
    14941 acaacttcgt tgtttgtttc gccnnnnnnn nnngtttcgc cctgctgata aagattcatc
    15001 agttctgtag tttcatcaat tgaaaaaccc agatcgagca aatattctgc taccagctgt
    15061 tcatcaccaa cggcataaga ctgaacaaaa tccgcttcct gcactgcctc atactcatct
    15121 gcaaatgccg gaacagctgt cgcactcaac gccgccgtca ttgccagtgc cgaaacacca
    15181 gctacggttt tctgaataaa ctttttcatg tttcattttt ctcctctaat tttaaatgac
    15241 agactttgta acttttttct ctgcaagcac tcgatttttc caactatcga cattactgct
    15301 tctgtggaca accccaaggc tataattttg gctgccacat tgatatgcag acacaatgta
    15361 actatggtca ctgcaatttc aattcccaaa acaatatatg tccgcatacg ataaacatgc
    15421 ttctctactt catctaaagg gttattttct gtttctacgg gcgataaaat cgcaatcacc
    15481 acacttgaaa tcaaaatcaa accgccagaa atccataaat ttatcgacac ccatcgcacc
    15541 aaaaccaaca gccctgcaat catcaccgta gatgacaagt agcactttac cggactgctg
    15601 gcatgatgtc cgcctgcaca catacgaatc ggaatatatg aggcgtttaa caacaggcac
    15661 tgccaaaact gacggaacac aaccccaaga aatatagttg tgacaacgtt tatcagcatc
    15721 atgaaaagct gaaaaaatcc gaagctgtaa atctctgcat cttctttctg aacaatcccc
    15781 agagaaacta atttttcggt aaacctctcc gaaagtttct ccagcatcag aacttccgca
    15841 tttcctttac agacttgggc attttcggct catagttcca ataagagcac gggaagttcg
    15901 accagaacga agatgtcttt cttgctgcct ccgccatttt cttccacact attttcgaat
    15961 tctttttcat ttaaaacatt cctctcattt tagatttaat aagatttctc ttacattttt
    16021 agtatagcag tccaactgga aaaaatcaat agctttgcac taaacgggaa taatctgcat
    16081 tattcgggaa tggggtaaca aaaacccgat agcatttttg tgcaaaatca ctatcgggtt
    16141 cgaatttcat ataattggct gagaatctat cggaatcgtg atagacacca caaagctgtt
    16201 attttcaact gtcgctttcc agattccatt atatttttta acggtcattt caatgttttt
    16261 taagccgatt cccgcatgtc catttttagt agacaaaaaa ctgttctccg tatttggatt
    16321 taatttttca tacttatttt ccgtgtgaat catacagcac tgcatttgtt cttcgattac
    16381 aaacaaaaca gttctgtctt tatccaattc cgaaaccgct tctattgcat tatctaaaag
    16441 attcgaaacg attgttgtta agtccatatc tgataaaaaa tcaagcttat catataaaat
    16501 attcaagtnn nnnnnnnncc tttctgttct gctttcagca actcatgatt taggataaca
    16561 gatagcagtt gattgctgca atgaaatgat gggtgcagtt tgtccagctg ttgatataac
    16621 gatttttcat actgttttgc ctctgtcaaa ttttcagtct gtatcaatcc ctctaaagca
    16681 tttacatgcc gctttacatc atgtatgatc gtcatagaat tttgataccg cttttcaaga
    16741 tcacgataaa ctgccatttg catatctgtt tgctgctgca acaatgcgtt tttcttttcc
    16801 atttcccgaa tcgcagaaac acgataaaat acaaaaacaa tgtagacatc tagcacaata
    16861 cagcctgtta aaaagaaaac caagaatgtt ccggaatgcg tatttgcaat attttctatg
    16921 atgtaattgg cagtgataat ttctccagtt gctaaaattg catatgagat cgattcatac
    16981 catacaaaac tactcttttt ctttcgaatc atagaaaaga caatgtgaca taaaataaat
    17041 gtaagcaaac aagttcgaag gcagcttgaa atcgttaaac tatcttccgc aaccgcagac
    17101 gaaatcgttt tttgtgtcac aaaggacaag ctcagcgttg acatagcatc agctataaaa
    17161 caaatcaatg caatcaggaa gtcataaatt aaaaatgaga tttttggtat acgaaaacaa
    17221 ataatcgtgg ataaaacaat cattgccaan nnnnnnnnnc ttattgtatt tagaatcgga
    17281 attccaaata tacacatcaa tccttgtaaa acagaaatta tcgtcacact aagaaaacgc
    17341 agccaaatct cagatttttg aatggagaaa aaattagata acgcccatat tccagaaaag
    17401 gtacaagtaa ggcttaccat acaactaaat aataatttca tcagaaacac ctcctatttc
    17461 tgcaaatggt gatgcaaata gtcacttatc ttttcatgga cttgttttct tctgtttttt
    17521 gcaattgg
//
LOCUS       DAWBGR010000033        17268 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1110_length_17268_cov_3.548481, whole genome shotgun sequence.
ACCESSION   DAWBGR010000033 DAWBGR010000000
VERSION     DAWBGR010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17268)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17268)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17268
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1110_length_17268_cov_3.548481"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7813..7822
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4207 a         4483 c         4083 g         4485 t
ORIGIN      
        1 cgaaaaattt gacgacgcat ctgtcgcaca atctttgtgc ggtattgccc taaaatttat
       61 tctgccattt ccggcaggtt gttgatgatt tgcaccaaga accggagcaa aacggttgcg
      121 tcattggtat ctgtcatgcc gtcggcatcg cagtctgcgt tctgccgtgc agtatcattc
      181 aaggttacca cgccggcagt cgccttgttc agcagcactg cgtctgtgat gtctactctg
      241 ccgtccagat tcacgtcacc gtacagcacc tgtcccagca cttcggaaga agatgcagtt
      301 gtctgagctg tggtttctgt ggtggaggca acggtttctg tggtgctctc cgaagaagcg
      361 gtttcacttg tggtgtctgc cgcggaggaa gtggtttcgg tttctgtggt agtagtttct
      421 tccgtggtcg tgactgtggt ttcggttgtg ccggactgtc ccagcacctg aatggtcaga
      481 acaacgtccg gacaagtggc aaatgtcaca tgcagggtgt gcttgccttc tgtcagggtt
      541 tccagatagg agcgattgaa gcggaactgg ctgccgttga cagcgtaatc cgttccctgc
      601 tgcaatgcat ttccgtcctc ggtaatgccg gtcagcacat gtccgttagt gataatggta
      661 aagatgatgt ctgtgggatc ctgcggatca aaggtcgctt ctgtttctgt tgcttctgca
      721 tcgggtaccg tgaccgtacc gtcatggtac agaccgatgt catcgaaata ccatacaccg
      781 gttcccggat tgccgttctg tccggtgtag agagaaaatt ctttcacacc actcagtgtc
      841 ggagtttctg cagcagtgcc ccattgctgc acatagaagt ctgagaacgg gatctttacc
      901 tctgtccagc cttcctcggc agtgactttc tgaacgctct cccagtatgc accggcaccg
      961 tccacaaact ggaatgtagt ttcgcggttg ctgccgtccg acaggatcca gaagcgaatg
     1021 ccgtcgaagc cggtccagtc tgcactgccc agattcttgg tggcaccgca gtagcctgca
     1081 ccgccgtttg ttacgctgta gtcgtatttc agggcagttt tgccattctt tacatgttcg
     1141 gtgtccagcg aaagggtcag gctgtcaccg ctggaattct gctgatatgc tgcggcaact
     1201 gctttgctgt ctgcatagct ttcgaagtcg tcgatcacgc tgcgggactg ttcctccgca
     1261 gtggaatcgt acacgcgaat caatgcggtc ggctgtgcac cctcggtggt aagcagcgtt
     1321 accttgtgat tgcccagttc cagcgattgc agtgcatttt tcgaaatggt aactgtagtg
     1381 ccgctgatgc tgtagtcggt gtcagcagtc agtgcggtat cgtccagctg tagtccggag
     1441 atgctgccca gtttgatcgt tgcctgcaag gtcacatctt tcgggttcac aatgtcaaag
     1501 tctgcctttt tcggagaaac gctgttctgt gcattgcggg cattcatacg ctctgcatgt
     1561 gccataatga catcacgtgc atcattggtt ttcgggtaat cccgaaaata aacggtatag
     1621 ccgtcatagt cctgatacag gctgccgccg tccatggaag ccagcatcca gtagttggtt
     1681 ccggcgaatt ctacgccctc gtaggtgttg ccctcgaaaa tgttgaacca gtctgtgtaa
     1741 acatcggctc tttcgccctg gtctttccag ccgaattctt ctgcgatcag cggcttattc
     1801 attgccgcgg tgtcctcgcc gtgcttcttg aaccagaatt cgccctgctc cttggtcagt
     1861 ccccactggt cgcagtacac atgcagagtg ccgaagtcaa tatccggaat cgaggtcagc
     1921 tgtaaatagt ccataccgga gctgccgtgg tatacatact tgtggtcgcc ctccggaaaa
     1981 tcgttgtaac catagttata gaagccttcg tcaccaacag ccagcatgtg gttgtcgtcg
     2041 atagacttca cataggcact catttcggtt gcccacttta caacggtgtt gttttcacag
     2101 cccgcatcgg attcacagcg aggttcattc gccagttccc atgagaagat cgtgggatcg
     2161 tccttgtact ttacgccggt atatacgttc tcatgattga gcagtgtctt gatgtaatcc
     2221 ttataccagc ccttaatggt ttcattggtg tagaagtcat cgtgaccgct gacattttcc
     2281 cctgccagct ttgcccactg gacatactgt cccatgccgc cgaatgcttc ccagttattt
     2341 gtaaaggtga tcagcagttt gataccttcc tgctctgcct tgtacagtgc atagtccagc
     2401 ttctgcaagc cgtcctcgcc ttcgttgacg accggtctgc ccagatcggc atcgaaatac
     2461 tgatagtaca cgccttcctt ttcgccggag ccatctacgt tgtcggtgaa tacggtgtaa
     2521 cctttatcag aaaccttatc cgttttcaca ccggcatcta agttgcccca tgtgcggaca
     2581 actttcagtc ccatttctgc ggcatcttcc agcactgcat ctacgctttc cttcggcttg
     2641 aagttcagat agtagttatt ggtgccggca tagtagaatg tagagccatc cagcatgaac
     2701 tgggtgccgt tggtggtaac gaatccgctg cgggtatcag ctgcatttac ggtgaatgat
     2761 gatgcaaacg gcatcattac ggtgctgcag ccgagaacga cactgctgat tgctgcgatc
     2821 catttcttct tcattttggt tcctcctcat aataattcca aaagagtgtt atccctgttg
     2881 atgcataaat tccagattcc ggtcgatcag atcacaccgc tgcttgacac aatcggcact
     2941 gcgaagcggg tcctgcgggg tgtgaaaggc gtaatccaac agtcgcttga tgtcggtagt
     3001 ggcaacgtgc aaccgtgtgt ctgatgatgc ataataaatg tataccgtgc cgtccggacg
     3061 aacgatcgca ccgttggtga acaccacgtt ggaaacatcg cccacccgtt ctccgcccag
     3121 cggagccaga aaatagccag acggttcggc aatcactttc cacggctcgt gcaggtctgt
     3181 gacaaataca tacaatacgt agcgaagtcc cgctgccgta ttccgcacgc cgtgtgccag
     3241 atgcagccag ccctgttccg tttcgatagg caccgcaccg gctccgtttt tggattcggt
     3301 aatggtgtga tacttccgca ggctggtcat acgttcttct tcaatcaccg catgggtgat
     3361 gtcctctgcc agtccgaacc cgataccgcc gccggaacca gtttcaatga agccgtccat
     3421 cggtctggta taaaacgcat acttcccgtc tacgaatttc ggaagcaggc agacgttccg
     3481 ctgctggggg ctttgcttgg tcaccagatt cggcaggcgt tcccacgtgt tcaaatcctt
     3541 ggtgcggaca atgcctgctg ccgccactgc cgctgacaag tcggcaggat ctttggcact
     3601 ggattcagaa cagaacacgc cgtagatcca gccgtcctcg tgctgagtca gccgcatgtc
     3661 atagacatta gtttcgtccg gatacagatc cggcagcaca acgggctttt cccgaaagcg
     3721 aaaattgtcg atgccgttgg tgctctctgc aaccgcaaag aagcttttgc ggtcattgcc
     3781 ctctacccga accacaagac aatacttgcc gttcagataa atcgctccgg cgttgaacac
     3841 tgcgttgact cccagccgtt ccagaaaaaa cgggttcgtt tccggattca gatcgtaccg
     3901 ccagtgcagc ggcacatggt cacgggtgag caccggattt ttccaccgca ggtagatacc
     3961 attataaaaa gaagatttct catttttgcg gcggagcaat tcctcctgct tctctttctg
     4021 ccgcagatat tcaacatgat acatctggat tcctccttat gatctccata cacattctgc
     4081 cgttatgata cggacacttc cacggatcaa caatcgtcag ctttttcacc ggtttgccgg
     4141 tgatgtccac tttccagaac cattcgctgt tttcccgcga gtccaccagc tgccgcagaa
     4201 tatacttcca cactttcgcg gcagcttctc cgtactgttt ctgttcaggg tgtttttcca
     4261 gcatgtggac aaatccaacg atcgcctctg actgcaccca ccagatccgg tctgtatctg
     4321 tcacaccttt ttcgatttcg ttgcagacgg cttcgccgtc aaagccgcgt tccagcacag
     4381 actgtgccag cacatcggtc atgcggtgta ccttttcaga cagtgccgca tctcccagca
     4441 ggtcacagcc ccagtccagc agccatgcac tttcaatatc gtgtccgtag ctctggaggt
     4501 cgatcagact gtggtagtcc ttgtcaaaga atacctcctg ccggcgtttt tccggttgga
     4561 acatatgctt tccccagatg tccagcaggg cacgcagttc tgttgccact tccggtgcct
     4621 gcgtcgcctg atacagtccg ctgtagcctt caaaaacatg gagcagggtg ttcatggtac
     4681 gctctgccat aacaccgttt tcggaaagct tttcgttaga ttccggattc cagaaacggt
     4741 cataggcttc tccatagccg cctgcatcgc ggcaatggtt ttccacgcac tgaagcaact
     4801 gattcgccgc aaacaacgcc tccggatttc cggtcagccg ataataagca gacagggcgt
     4861 agatcgcaaa cgcctgattg taagtgtgct tggtggtgtc cagcggtttt ccatcaaaag
     4921 tcaccgacca gtacacgccg ccggctttgc gatccatgca gtaccgcatc agaaaccggt
     4981 acgcatgatc tgccgcctgc cgcaggtccg gacgtttcag cagcattgcc gcttcggaaa
     5041 aaaaccagag gatccggctg ttaagaatac agcccttttc ggctttttca ttccgccgca
     5101 ggttcagatc cacataactg acaaacccgc cataatgatc gtcctgcagc gacagccaga
     5161 acggaataat tttttgttcc agcatcattt gcacttctgc ggtcagtttc atgcccatcc
     5221 ctcctattgt tggcatatct gcaattatcg tctgccctcg ctgcagtcat catcacaagc
     5281 cgcatcatgg gactgaatgt attcgactac agtctgtaca tcagtaaacg ccagacccac
     5341 atagctgtct gcacagccat agtaaatcgc aattctgccg gtgtcgccgt cctgcagggt
     5401 ggcacagggg aaacaaacgt tcggcacaaa gccacgttct tcgtaccact gttccggcgt
     5461 cagcaggaag ttctcacagc ggtatttcac cctggacggt tcgtccaaat ccagaatggc
     5521 accgccgatg ctgtagacga atccgttaca ggtattggta acaccatggt agaacaacag
     5581 ccagccctca ctggtttcga ttggtgctgc tccgccgcca attttggtgt tttcccacca
     5641 gcggctgctc ttgcccataa catggcggtg gtgtccccag tattccatgt ctttgctttc
     5701 gctcaggaag atgtccccga atggggtgtg tccgctgtca ctgggacggg acagcatcag
     5761 atatttgtcg ttgacctttc gcgggaacaa taccgcgttc cggttaaacg gcaggaacgg
     5821 attttctacc cgcacaaatg tccggaaatc ggtcgtcttt gccatgccga tcgctgcacc
     5881 atatgcgtcc tgacaccaga tgacataata ggttccgtcc acttctacca gtcggggatc
     5941 gtatgcgtag atcggctgaa acggctcgcc gttctcatct acaaactgga tcttatccgg
     6001 ttcgaactcc cagtggattc cgtcgcggct tctgccgaag tagataaacg gaatgccgtt
     6061 cacctgctct ccgcggaaca cacccacata ggcaccctga tagggaatca cagcactatt
     6121 aaatatgcgg gcaacatcgg gaatcggatt gcgggaaatg atagggtttt cggtataacg
     6181 ccacaccggt gcagaacagt ttcccggtcg gttctgccac ggaatattcg gcaggctggc
     6241 tgcctgtgtc aaagtcgggt tcatatatat tgcctcctat tattccttta cagcacccat
     6301 tgccatgccg ctgtagatct gtttctgaca aaacaggaaa ataatcagaa tcggaatcat
     6361 ggtgataatt acgccggcac agatatactg atactggctg cccataggtc cggtgaatgt
     6421 gtacaaagaa gtggatacca cctgatacag gttcttgtcc tgcaagtaca ggtttgccat
     6481 atagtattcg ttgtaggtgc cgacaccttt cagaatcgca ctggtaacga tcgccggttt
     6541 cagcagcggc agcaggattc ggaagaatac gccgaaataa ctgcaaccgt ccagaattgc
     6601 cgcctcgtcc agtgtctttg gcagattctc gaaaaactgc aaaaagatat aaatggcgat
     6661 aacatcagtc cccagcatca ggaaaatata gccgtaaagc gtattgacca gatgcagaac
     6721 cgtcatgatc tgatagacgg tgacctgtgt ggcaattccc gggatcagtg ccgcaaccat
     6781 aaacagattg cgaatcaggt gattgccgcg gaactggaat cggttcagca catacgccag
     6841 catggcggaa atcatggtac tgcccaccag cacacaaacc agaatcagaa agctgttgag
     6901 aaacgctttt cccatgtctg ccttttccca tgccaccttg aaattctcaa agttcagcca
     6961 gctttccggc agtgtcataa cactggtcgt gcggtattct tcatcgtttt taaatgccgt
     7021 tgtcacacag ctgacgatcg gcaccagtgc acagaatgca cccagcagca gaatcgcata
     7081 ctgtagaatt tgcttcggca gtttttttag aaagacgtgt ccctgtttca ttttctcgcc
     7141 ctcctcttac tggtgcggac accagaatcc aacttctttt ctgccagctt ctgcacagca
     7201 ctgaccaaca ggatcagcac cagcagtacc actgccattg ccgatgccag accaaccttc
     7261 tggttggcgt gtgccagctt atccatcaag acaaaatagg tcgatgttcc gaagccgccg
     7321 tctgtgatta cataagggat ttcgaatacg gacagcgaac cggaaatggc gaggatcata
     7381 ttcagtacta cgatcgtgcg aatggacggc aggataatgt accggaaccg atgccatgcg
     7441 ttggcaccgt caatggctgc ggcttcgtac tgagttttgt ctacgctggc aattgctccg
     7501 ataaacagta ccatgttttg tcccatgtac cgccagatag aagatgccac cagcacccag
     7561 ttgttgatgc ggatatcttt cagccagaac ggcagcatgt cttcaggaat tcccagcaag
     7621 cccaaaatgg agtccagcac gaatccgtgg gtatagaaat acttgaaaat gaatcccacg
     7681 gcaatgccgc agaccaaact cggaaagtac agtgctccgc ggaaaaagtt tccgcctttt
     7741 ggattaaagc tgagaatcgt tgcaaacagc agtgccaatg ccagctgcac aacggaacca
     7801 accatataat acnnnnnnnn nnatataata caggctgagt ttcagggcat tgaagcaatc
     7861 atctcgtgtg aaaacgtcca catagttttg cagtcccaca aactttctgg caccgatata
     7921 tttcatgtcg taaaagctga actgcaccat tttgaaaaac ggcagatatg taaacagtac
     7981 cagcaggaac aatggtacaa acagaaatgt cagagtcact accacttttt gtacattcac
     8041 attggaccat ttcagccgcc gcattcgttt ggacaatccg acttctgtgt gatgtgctgc
     8101 catgtccatg cacttccttt ctgacttcaa gcgttatctt tattccggag ttacaccgtt
     8161 cttttcctgt gcactggtcc atgcagcatt ccattcatcg gcgatttctt ccatcgtttg
     8221 attgccgccg atggctgctt ctataatcga agccacatgc gtggaatcag aatccagaga
     8281 aatttcagaa tcgttattga tcttaccgaa caagtcttct tctccggcag gagcaggatt
     8341 atcggtcacc agcgtgatac tctgaaagct ttccagagaa tccggatacg catcgccctt
     8401 tacgatcggg ataccgccct gagttttcgc aaagccggag gactcggtga gccacttgat
     8461 gtacagcatc gctgccgttt tttcttcgct ggtactgttc acattgacac catagcaata
     8521 gtctgcactt gcggaagcat actgtttgcc gtccacgctg atcgggaacg gcatataggc
     8581 gatgtcatct gcattgggac cgcctgcctg catctgcggc actgcccaac tgcccagaac
     8641 catgcagcca atattgcctg cgttgatctg tgccttacag ccctcccagt cagttgtagt
     8701 cgggtcttct tccgtcagcc cctgctttac tgcctcatac agagtatcat atacggcaaa
     8761 cggtccggtc atatcgtcat gctttgcaaa cggatccttg gagtgtacca gcttgttgtt
     8821 tttgaaatct gcatctcccg ttgcaccgcc gctgatataa gcatcccatg ctgttaatgt
     8881 ccagccggca gcgtagttgg tatacagcgg aatggcatcg gttttttcct gaatcagctt
     8941 cagatctgcc agaaatgttt ccggatctgt cggcagttct gtgatgcctg ccttttcaaa
     9001 aaccgcttta ttgtaaacga caccgctgac attgcccatg gtagggatac cgtagacttc
     9061 attattaaac atctgcttat ctgcaaattc gtaagtatcc ttgaagtcac cgtagtcacc
     9121 gatcttttca aaataattac tcagttcgct cttgtctacc gtagttggaa tacagcagat
     9181 gtcaccccag tcaccggaag acagccgtgt tgtagcatca gtatcgtaat cggtaatacc
     9241 ttcataggta attgtgatgt tcggatacat ggcattgaat tcgctgatgt agtcctgaaa
     9301 atcggaatcc accagatcgg tgcggtttgt caggatcttc aggtctgcct tcagatcagt
     9361 agccgtttcg cctacctgca tgtccttata ggcaacggtc ggctttgcgt ctgcgttttt
     9421 gctggttttg ctgttggtgc ttccacacgc ggtcgccgca gcagtgagca tgcctgccag
     9481 taccagagcc agaattctgt gtttcataat aagttcctcc atttcggtat tgcgttctcg
     9541 tgctgaatcg caattttatg ctgatttttc acgtataggt ttccggaatc ttttttatat
     9601 tccgtttctt tatgatccta tcttactcta ttgttaccta aaagtcaagc taaatttaag
     9661 tttaaaacag cttttctgca ttttccacaa aaggattcta ttcgaattag ctatattcaa
     9721 caaacatttc accggatttg ttgtgcaatt cggtatcgag attcgggatt tccgattttt
     9781 ttagctaaaa aaataagtcc gacagagaaa ttctcccctg tcagactgac atataagcaa
     9841 aattgttcct aaggaaacac tgaataaagc ttgtgcgtaa gatgcgtagt cagatttctg
     9901 agcaatatga cggaaaatct gcaagcagat tgcgtgcaaa gccgtcaggc ggactttatg
     9961 cagtgtttcc tgaatgtttc tgcacgcttt cccgaatcac cagttttccg ctgacgacaa
    10021 tgcggcgaac ttcggcttcg ggcttctgga tccggcgaat cagctgctga gcactgactt
    10081 ttgccatatg atctacgtca atggcgtaag tcgtcagcga tggaacagcc agatctgcca
    10141 gaatgtagtc atcgaatccc accacagaaa cgtcctccgg cacacggatc ccattttctt
    10201 tcagagcatt cagcagcaca tatgcagtaa catcacagtt acaggcaaac gcagtgggca
    10261 gcttaggcag acgttccaga gaaactgtca cctttccttc tgcatcacga tccggaagaa
    10321 tacgatcttc cgtcacagtc acactagctt cccgcatggc acggcaatag ccataatagc
    10381 ggtcagtgat actgctggtg gcattgacag aacccacaaa gacaatatcc cgatgtccca
    10441 tttgcagcag gtaatccacc atggtataca tgccgtaata gccgtcggag atcacggagt
    10501 cccgatgata ccgtgcgtca aacgcgtcca gcactaccaa gggaatttca gtttccgcca
    10561 acatatttcg ataggctttt gagaaattgc ccatgacgat cacgccgtcc acccgttctt
    10621 cctgtaccag ccgcggcacc tgacagtttt gctccgcagt atcgttcacc acttccagaa
    10681 tgccgatatg tccggtctgt gccagataaa acaggatccg ctcgtacaga ctccagtaaa
    10741 acgactgtcc tttttcaata tatcgataag atgtcacaat tcccagcgta taggttttcg
    10801 ccgcagccac cggcttggct ttcttgttct tgtaacccat ttcttctgcc agctgtagaa
    10861 tttcctgccg cagctcctcg ctgacaccgt cctttccggc aagtgccttg gaaactgtta
    10921 caatgctgac atgcagccgc tcggcaatgt cagacattcg tactgctttt gccacgtttc
    10981 tccgcccttt ctttttgcaa agttgctttt ttagctaatt atacagtaat ttaagtaatt
    11041 tgtcaagaat tatttttcga acagcagacg aatattttgg gaaaagctga aagaaccgct
    11101 gtacatttct gtacagcggt tcttgtctgg tgtaagtgat acgtgaaatt ttaccaaaaa
    11161 acatatatct gaaaacattc gaacaccatt tgcattttct ccaagcctat gctataatat
    11221 agttgtattt tatcattagg agaaaagcta tggcagaaat gatactggtt gtcgatgatg
    11281 aaacagaaat tgcagacctt gtggaggtct acctgaaaaa cgaaggctat ctggtgaaaa
    11341 aggcttcctg tgccgcagag gctctgcata ttgcagacac ccagcccatt gcactggcac
    11401 tgctggacgt gatgcttccg gatctggacg gcttcaccct ctgccggaaa attcgggaaa
    11461 agcatttgtt tccgttgatt atgctgactg ccaaaacgga agatgtggat aagatcacag
    11521 ggctgacact gggtgcagac gattatatca cgaagccgtt caacccgctg gaggtcgttg
    11581 cacgcgtcaa gacacagctg cgacgatata tccggtacaa ccagactgca ccggaacctg
    11641 ctccggcggc agtgtacgac tgcggcggac tgtacatcga ccatgaaagc catacatgca
    11701 ccctctacgg caaaccgctt tcgctgacac cgctggaatt cgagatcctc tggtatctct
    11761 gttcccgcaa ggggcatgtg gtttcttccg aagaactgtt cgaagcagtc tggggcgaaa
    11821 aatatctgga caacaacaat accgttatgg cacacattgc ccgcctgcgg gaaaaaatgg
    11881 gcgaactgcc ccgcaagccc aagtggatca aaacggtatg gggcgtgggg tacaagatcg
    11941 atgaataagc aaaacagcaa acacaaaaaa ctggtacagt tctggctgtg cggcatcggc
    12001 ttcaccattg gcatggtggt cttgtttgga ctagggtata ttctctgtca tctggtcaca
    12061 tggcagtcct acgaaacgcc ctatatcctg ttgaaaatgg cggaatttgg catcgcacca
    12121 gtactctttc tggcaggctg gggatttttg atttaccgtt ttttcttcta tgtccccaga
    12181 gcagtttctg accgttcccg tgccattcgg cggctatggc tgcgaacggt actgctgtta
    12241 tttctgtcga tcgtgctgtg gctgggacta atgattctgc tgcttgtcgg catttcgtct
    12301 ttgcttcgct gggatattgt ttggaacggt tcggcttcca tggcatatat cttgcagaca
    12361 atacgttttt tgactccgtt tctggcaatt gtcggcgtgg tcgtcatttc gctgctgctt
    12421 tttcgaaagc cgttctccta tttacaggag atcgtacaag cagcagagca aatgcagcag
    12481 gaaccggaaa aaccaattac cctttccgca ccagtcaaga cgattcagga cgaactgaac
    12541 cgcctgcggg aatcctccaa ccaaaacgca cttgccgcaa aggaagccga acagcggaaa
    12601 aatgatctga tcgtctatct ggcacacgac ctgaaaacac cgcttaccag tgtgatcggc
    12661 tatctttctc tattgcagga cgagccggaa atttcacagg aactgcgtgc aaaatatacc
    12721 ggcatcgctc tgaacaaggc acagcggctg gaagaactca ttaaccaatt ctttgagatc
    12781 acccgttttc agctgacgca tatggaactg gaatgggaaa cgattcactt ttcccgtatg
    12841 ctggagcaaa ctgcctttga atttcagccg attctggagg aaaagcacct acagctgcaa
    12901 acggaaattg cccctgatgt gaaacttacc tgtgatccgg ataagctgga acgggttgta
    12961 gacaacttga ttcgcaacgc tatcagctac agttatcccg ataccgccat ttcactggag
    13021 atgcacacca ccgaggaata tgccgtgctg acgatctgca ataccggacg cactatttca
    13081 ccggaaaagt tagaccgcat ttttgaacag ttcttccgcg gggacagttc ccgtgccact
    13141 gctaccggcg gggctggatt aggattggca attgccaagc agatcaccga actgcacggt
    13201 ggcacaataa aagcagacag ccggaacgag cagattcgat tcaccgtaac gctcccgctt
    13261 cgtcagaaaa tcgtatgatt tttttcagaa aaaaaacaga aagccatatg catttccgca
    13321 ggcacttctt tcgcagatgt gatacaatca gaagcgaaag gagtgctttt tatgacttca
    13381 caaagacaca gattttttcg ttttctgttt attcccctgc tgattctggc agtgcttctc
    13441 ggactggtgc tgttccgaca gcatgccggt ttacctacca gattttccac gcaaagcaac
    13501 accccgtgga atctgatact ggtcaacaat gaacacgcct tgcccagagg atactcgccg
    13561 gaactgacga cactttccaa cggtgtgcag gtggattccc gcatctaccc tgatttacag
    13621 agtatgtttg atgacatgcg aacggaaggt gtctaccctg ttgtcggcga gggttatcgt
    13681 tcggagcagc agcaacagga gatgatgcag gaaaaaatcg atgcctacct tgcagagggc
    13741 tacagtgaac gcaaagcacg caaggaagcg gaaaagtggg tggcaaagcc aaactacagc
    13801 gagcatcagc tgggactggc actggacatc aacgccgaca agagccggtg cgacaacgac
    13861 accatttatc agtggctgtc acaaaacgcc tatcgctacg gctttattct gcggtatccg
    13921 gagggcaaag aggacattac cggcattgac tacgaaccgt ggcactatcg ctatgtggga
    13981 aaagaggcag cacaggaaat ccagtcggaa aatatttgct tggaggaata cttagaaaag
    14041 acatgactgc tccggcaaat ccagcaaaag tttttctctg ttctccgatt cccacaaaaa
    14101 caattgacat ttcccacaaa ttgtgctaca atataggcaa aacagaaatc agatgcggaa
    14161 gcattccgca cgcacatgat acagggagga ttttatgctg catcatattt ttcagaaatt
    14221 gctgctgccg gcagttttgg cgggaaccat gcttgccggc acagtggctc cggcggtgtc
    14281 cctcgcggca caggccgcca cacagccgga cagctaccac gacgactggc tgcacgtcaa
    14341 cgacaacgcc gaaatcgtag acaaggacgg caatccggtc tggatcaccg gctgcaactg
    14401 gttcggctac aatgtcggca gtcaggtttt cgacggggta tggtcccaga atatgcacga
    14461 tatgctccgg cagatcgccg accacggatt caactttctc cgcattccca tgtctacgga
    14521 aattttgctc cagtggaaaa acggcgaccc tgatcccgct acccccaaag tcaaccagta
    14581 caccaatccg gaactgacgg aagaaggcat cgagggcggc acgatcaagt acagcttcga
    14641 catctggaac atggcggtga agtggtgtcg ggaactgggc atcaagatca tgatcgatat
    14701 tcacagtgca gaaacggctt ccgccggtca tcagatcagc ctgtggtata cggacaaatt
    14761 cagcacagag gactggtgcg atgcactggc atggttcgca gactactata aagacgacga
    14821 caccattctt gccattgact tgaaaaacga gccccacggc acagctgatg taaaagacca
    14881 aatggcaaag tgggacgatt ccaccgaccc taccaactgg aaatacgccg cagaaacctg
    14941 tgcggcacgg gtactggaaa agaatccgga actgctggtc atggtagagg gtacggaagt
    15001 ctaccccaaa gagggctatg actggactgc tccgaaaatc gactacacca ccatgacaga
    15061 atattattac ggcacatggt ggggcggcaa tttccgcggg gcgaaaaaat atcccattga
    15121 tctgggcaaa tatcagagcc agctggtcta ttcccctcac gactacggtc cgctggtatg
    15181 ggaacagaag tggttctacg acggcttcac acaggaaacg ctgctgaaag actgctggta
    15241 tgacaactgg ttcttcctcc aggatgaggg cgtggctccc ctgctgatgg gcgaatgggg
    15301 cggatttatg gacggcggca aaaacgagca gtggatgacc tatctccgtg actttatgat
    15361 cgaaaaccgg attcaccaca cgttctggtg cttcaacgaa aactccggcg acaccggcgg
    15421 actggtctac gacaacttcg gcaagtggga cgaggacaaa tatgctctgg tcaagccggc
    15481 actgtggcag gacgacaacg gcaagtttat cagtctggat cacacaattg ctctgggtgc
    15541 caacggcatt tccctttccg actactacgg cagcgggaac agcagtacaa caacaccgga
    15601 cagcaaaata attgcaggtg atgtgaacag tgacaagctg gtcaacggtg tggatctgac
    15661 actgatgcgg cagaacatca ccaaatggca gagcacagag gatgtgctga ctgccagccc
    15721 gcaggacacc aacggcaacg gcgtattcag cgtagcggat attgtcctgc tgacacagta
    15781 tctgctgggg aaagatgtca cactgaaaag ctacacttcg taacaatacc attctataaa
    15841 aaagccccct gactttcacg gtttgaacca gaaagtcagg gggcttttat gtacataata
    15901 aagctatatc agacaaatgc ttccctgatg ctgtcatagt gcaggaaaat accctgtgca
    15961 gcaatggcac gaatctcctc ggcagttgcc agtgcgaatt ccgtcacctc gtccggctgt
    16021 acttttacat ctgcttcggt aaaatccagc acgtacttgt aaatatccac aaagtaattg
    16081 ttctgttctt ccggattgac acgcttatag gaaaacgcat agccgccctt ggcaccggtg
    16141 acatcaatac cagtttcctc cagaatctca cgttttacgg catcggctcc gtcttctccg
    16201 gcacgaacac cgccgcccgg aacttcccac cagccggcag cccattcctt gtccagatta
    16261 cggcgtgtaa tcagatattt gccgtccgga cgctgcaaca ctcccagcac ggtcaggtga
    16321 aattcgtccg gcttcatgtg ccagtcattc cggttcattg tgcgtccggt cttttgtttg
    16381 ttttcatcgt aaatatccca ctgttccatg gatttttgct cccttttctt tttttgcctt
    16441 tttattgtag catagttgag gggcggttgt caaggcgaat ttgatttttt gctattgcat
    16501 ggaataaagg aaacgctgaa taaagcccgc ctgacggctt tatgcagtgt ttccttaact
    16561 cttgtttgct gtacataaca gttaattatc cctttccaaa gtgatatgat ttaaataatt
    16621 tttgctgtgg tgatgcttcc ctcatgataa aattctatta tccgcttcat atcagttgat
    16681 attagccaag tatcaaacga aacggctaat acatcataaa tattatctaa aatatcctca
    16741 atattgcact tgatgatcgg aattgctgcg ttatcccata tgacataata ttcactttca
    16801 tcaggtaact gacggatttc ctcaaagtta atttcatgag cgtccgcata ctttgaaaaa
    16861 tcaattttcc cataaaatgt agactttaaa ttggaaagaa tttgttcttc cagttcctta
    16921 tcctccaagc attcttccaa taaagtcata tcagttgcct ccacattttg tgcagtgttt
    16981 gtttgtttgt ttgtgtatag tcatgtgctt tgcttcatga atattctatt gtattatacc
    17041 ataattgagt ggggaaagtc aaggtggtga ttctatgttt cggtgcaaac gtgtgcaaga
    17101 aacaggcaaa ttgatgaagt gctcttttca tacgaaaaaa ttacaaactt taggaaacac
    17161 tgaataaagc ttgtgcgtaa gatgcatcgt cagatttttc agctttataa aaaacggctt
    17221 aaatgcgtcg aatttcgcat ctaagccgtt ttgcatatgg tcgaggtg
//
LOCUS       DAWBGR010000034        17251 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1112_length_17251_cov_4.787218, whole genome shotgun sequence.
ACCESSION   DAWBGR010000034 DAWBGR010000000
VERSION     DAWBGR010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17251)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17251)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17251
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1112_length_17251_cov_4.787218"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6218..6247
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4815 a         4146 c         4507 g         3753 t
ORIGIN      
        1 agctggcagc cgcaggctga ctgagggatt atctcgtgga atctgaaaca aaagggaaag
       61 attttggtag aatccctcca ccgtctgcga cggtccccct cgtactgagc ggtcccctct
      121 gccctgcggg catctcccca ccccgtgggg agtcaccctt ttcaagggag gcagttgcaa
      181 gttgcttaaa ggatttgtta tggaaaaaga ttatcatttt actggctaca ataaaaaatt
      241 gtgcgatact gctcgaatgc ttcgaaaaaa catgacaaaa caggaacgac atttgtggta
      301 tgactatttg agaaaaagtt ctcctacatg gcttcggcaa cgcgtgatag accagtacat
      361 tgttgatttc tattgcagta aggcaaagct gataatcgaa ttggacggct ctcaacatta
      421 taccgtggag ggtgaggaat atgacagcgt tcgaacggaa gtgctggagc agtacgggct
      481 ggaagttatc cgattttcaa atgatgagat tgacagacac tttgatgatt cgtgccggta
      541 tatcgaagaa aaaataaagg aacgaaccgg cgaatatcct gtgtatcgtt gaagaaagta
      601 aaaaggctcc cttgaaaagg cacccgtagg ctgactgagg gattatctcg tggaatctga
      661 aacaaaaggg aaagatttca gtagaatccc tccaccgcct gcggcggtcc ccctcccttt
      721 tcaagggagg cagaattcaa ggtgcagaaa aggctccctt gaaaaggcac ccgtagggcg
      781 tgccttgtgc tgagctggca gccgcaggct gactgaggga ttatctcgtg gaatctgaaa
      841 caaaaggaaa agatttcagt gaaatccctc caccgcctgc gacgttttcc ccttttcttt
      901 tcaaggaagg caaaaaaacc acaccaaaaa gaggtgaata cagtgccaaa ttacaaatat
      961 cgtgccacag ccatgagcgg aaaaatggtg cgtgggacgc ttgctgcttc cgatccggag
     1021 gcactgctga acgaactgaa aaagaaagat ctgttcttgc tggactacac ccagaccagc
     1081 aagacttcgt tcggctccaa gctgaaagcc aaggaactga cggacttttg ccgccagctg
     1141 ggcagcctgc tggacgcggg cgtttcggtg atccagtcgt tcaatattat ttccaatcgt
     1201 gccagcattt ccaagtttgc gagaaaatgc ttctctgaca tcaccaccga cctgaaacac
     1261 ggcaaggcgt tgtccgatgc aatggcagat cagggcaagg tgttccccga actgctcatc
     1321 agcatggtgc gtgccggcga agcaagcggt aagatcgggg atacgtttat caacatgggc
     1381 gagcatttcc agaaacagca gcgtatcaaa tcgcaggtca agggtgctct ggcgtatccg
     1441 ctggtgctgg tcattctgct ggtgctggtc attattgtaa tgtttacgtt tattctcccc
     1501 atgttcggcg atatgttcac cgatatggaa ctgccgctga tgacacggat cctcatgggc
     1561 atgagcgatt tccttaccca ttattggtat gtggcgatcg gcgtggtggc ggcaattgtg
     1621 gttgtctttg tcatgctgat gcgaacgccg gcttcccgtc tggtgatcga ccgcatgatc
     1681 gtgcacctgc caaaaatcgg caatctggtg cgtatcgtct atacggcaag cttttccaga
     1741 acgctggctt cgctgtatac cagcggactt tccattttga acgccctgca gatctcgcgg
     1801 gacaccgtga acaacagcta tctccgttcc cagtttgacg actgtatcaa gagcatccgc
     1861 gtgggcaacg ccctgtcgga atccattatg gcaatggacg gcttcgacag caagctgggc
     1921 gataccatta aagtcggcga agaaaccggt aagctggacg caatgctgct ttccaccgcg
     1981 gacgactacg aatatgaatc cagtgccgcg atcaagtcca tgatgtccat tatcgaaccg
     2041 ctgatgatcg tcattctggg tgttgtcgtt gccggcgtta tggtttccgt tctcgttccc
     2101 atgtactcca tgtacggcaa tatcgacgaa aacaccggtg cggtcttctc gtggctcgca
     2161 cagaccgcag gacgattcct gaaccggttc taagagaaaa agaggtgaat ttccgtgaag
     2221 aaaaaagatg tttctgcaca cgtgtatatt accaacagta aaatacaggt gctgatgggc
     2281 agctgtgtga aaaataaatt gcaggtggta aactgctatg aagaaccgct ggaggaaggc
     2341 tgtatcctga acggcattat catgaacaac tactctctcc agaatactct gacggaaatg
     2401 tggcagaagt acaacctgcc tgcaaagggc gtgacgctgg tggtaaacgg cagttccatt
     2461 accgtaaagc ctctgaaagt tccgcagatc gctccgaaaa atgtgcccgg ttttattcgc
     2521 ggggaactgc gggatattga gggaattcag aatatgctgg tggattattc cgtggtgacc
     2581 ccgaaaaatc cggacggctc ctgtagtatt ctggcggtgg cttccaccag ggagttcatc
     2641 accagctata tcagcctgtt caaagaggcg aagatcgaac tggaagtcat tgatctggtg
     2701 cagaactgtc tgatcaaaat gatgaagcgg ctgaaatctt tacagggaaa aacctatgcc
     2761 gtgttcattc tggataagaa tatgctgatg cagtgtctgt tttccaatga caacttcgtc
     2821 atgacccgcc gcagccggat tctggcagac cctaccgatg agggcttcga acgggaagtg
     2881 gggcagaata tcaacagcat tatccagttc aacaagtcgg aacagaccgg ctctgatatt
     2941 acggacgttt atctgagcgg ctttccggaa gatgctgtgc agatcttccc gcacttcgcg
     3001 gaaaatttcg gtgtaaatgt gcgggcattt ccggagtttc tccctacgga actgactgtt
     3061 ccgccggaga tgaatcccag cgagtacacg gtgctgctgg gcagtatgat acgttattcg
     3121 gactaaatcg gacaggagga atccaaaatg gcaaaactac caaaaaagga tattaacctg
     3181 tatcctcttg tcgtggcacc gaaaaagaac accatgaaaa aaggtgttct tttcggcata
     3241 ctggggacag tggcagtgct gctgctgggc ggcagttatg ccggtgcacg gatctacgtc
     3301 cacacccaag agaatctggt ttcagaaatg gaaaccaagg caaacgatac cgcattgatg
     3361 gaaaagatca acaacgccaa cgctgtggcg aatgacatca gcacactgcg gactgccggt
     3421 gatgtttata ctgaggtgcg ggctaccatt gacgggacac aggagatctg cgacaacttt
     3481 tccgaagatc tggtgcataa gctggttggc tgtgagaaaa cggtcagccc cgacagagga
     3541 gaaattcagg tggcagagat cactggactt tcctatgacg gcagcaccct ttccattgcg
     3601 gcaagttcga cggacagcca gtatgtttcc acatttgtga cgaatctgac acggctggac
     3661 ttgttctcct cgatcaccta tgacggctat tcgctttcgg acagcgtgta tacttataca
     3721 gtgaacgctg tattcaagtc ggcggaaact actgcaacag atgccgacgg caatcctgtg
     3781 gaaacaacaa cggaggtggt acaatgacaa cggaaattac ccgaaagcag gcagttctgc
     3841 tttctgtgct gggtgtcgta ttggtcgttg tactgtttgt acagcttctg atccgtccgc
     3901 tgatgacgaa agtctcggca gcaaaggaac agctggaggt tctgaaccag cagtattccg
     3961 acctgttgct gcaaagtcag agttatgacc agaatattgc ctcactaaag gattggaaag
     4021 agaaaaatgg cgaggaaacc aagaagctgt ttccgctgag cgatgcggaa cggatcgacc
     4081 gttttcttac ctttgtgatg aatgaagcgg gtgtcactgt cgatggcttg aacatcgggc
     4141 agaccatgca gtattatatt gacggtgagg gcaatctggt gactgcttcg ccggacgagg
     4201 tagccggtac cacagacagt tctgcctccg gaacagatgc ttcggacggc agcacaggtg
     4261 acggcagcgg tgctgcatat accgccactg gtgaatactg ctgcgacttt acctatacga
     4321 tggaaggcac ctatcagaac atgaaacaaa tgttggactt tgtcaatcgg gtgtcgtttc
     4381 tgggcgttac tgcgtattct ttcaacagtg tggaaaagcc ggcagcacag gaacaggctg
     4441 cggcaaacgg tgatgcggca aatgcggata aatttcagga ttggtataat tttaccatga
     4501 cgattaccgc gtacatgtat cagagtccgc tggaaacaga ctatgcagat gatgtggatt
     4561 ccgacgaaac caccgaagaa gcggcggaaa aagccatgca gtcggcaaca gcagacattg
     4621 cggcggaaga aatgacaacg accacgatca taaccacagc agcagatgct gcataaacag
     4681 ggtgatgcca atatgaaacg gaaaacagca cagcgaaacg gtgcagcgat tttgtgggcg
     4741 gtgctggtca tgctgatgat ctcactgatc tcggcgggga tcattcttgt agaccgtatg
     4801 tattacatgc gggagcagga ggaaaacaac cgcgtacagg caaagttcta tgcagaatcc
     4861 gcgatcactt tggtacagca ggacattctg aaagagggta cggtgacgga cgaaaccacc
     4921 ggggcaacct atcagagtat tttcgtcagc gacggcaaca ccgcggaaac ctttacgatc
     4981 tcactccccg atgcggggaa ctggaaatgt acggtaacgg tgaatcactc tgtggtcaac
     5041 gacagcgaaa ctgatgccaa caaaaggcgg ctgtccggtg agatctatct cacggcaaag
     5101 gtgacgcgga acactgccgg cagcggggaa aaggaattgt cagaagtttg tggaaagctg
     5161 tcagttcccg cgaatgcaac ccagtggcgg tgggacggct attatcaatt gtaaagggag
     5221 gcgtgacaaa atgcggaaac tgcgaaaagg aaccactgtt gtggaagtgg tgattgcatt
     5281 tgccatgatc tccatgtcct ttgcaatcgg catgatcggg atcgcttccg gctcgaactt
     5341 tctgaactcc ggtgcgaagc tgaagaacca gcagcggaaa aatgccgctg tggatctggc
     5401 gggagctgct gcggaaactt ctgatactac gattacagta acatatcggg accgcacaag
     5461 cggggacgaa aagaccaaaa cttttgatgt gcagaggcgt tccgcggaca atttcattca
     5521 gtatcaaccg aaaccgtaag ggaggaaagc agatgcggaa acttgtgaaa aaccgaaaag
     5581 gttttacgct ggcagaagaa gtggttaccg ttctgctgat cggcattctg gtggcttctg
     5641 cttcgggaat cctgctgaat gccatgcgga tcttctgcca gaatgtcatc atgttcaatg
     5701 cccagtccaa gggaatcgcg gtcatggaac agctggaaag tcatctggcg tatgccaaga
     5761 ccatcggaca gcaggaaaca gaaacgatcg ctgctaatga cggctgtgcc catcaggtcg
     5821 tattgtatgt ggacacacaa gacggaaaga gcgaactgaa agaaaaaggg acaatttact
     5881 attcagacac cgacagtgct ggaacaacgg tcgatcaggt gctctgtaag ctggggactt
     5941 atcaggcaaa gatccttgtc caaaaagtcg atgatgtaac agtgacagta gatgtgcgga
     6001 tctcgcggaa cgatactgtg tactactcgg aaaccagaat ggtcaagctg caaaatcagg
     6061 caacggtaaa ggcaacgctg aatgcctccg acatatacga cagttctgtg gcagcttcgc
     6121 ggtatttata catcggcagt ctggaataaa acatagggca atgccgcaca aagattgtgc
     6181 gacagatgcg tcgtcaaatt tttcgtcaga tgaaacannn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnngta ttgccatagg gcagcaccgc acaaagcccg cctgacggct ttgcacggtg
     6301 tcgctataga aacaatagaa aaaggtgaga acattgcaga atttacccat tggtgaagtg
     6361 ctgaaagaat atggctatat cacacaggaa cagatcgatc aggcactgca attccagaag
     6421 acacaaagcg gcaagaaggt gcgtttcggc acactgctgc aggaaatggg atttgtcaca
     6481 gaaacacagg ttctggaggc actggggaaa aagctggatc tccagatggt caatctggag
     6541 gagtatgagg tcaatgtaga cgtggttgcc aaggtgccca agcagcttgc ggaaaaatac
     6601 aacgtcattg ccctgtcgga aaacgacagc cgtctacagc tcgccatgtc tgacccgctg
     6661 aatttctacg cacaggaaga tattcggcag atcgtggata tgccgctgga agtgctgctc
     6721 agtgaaagtc cgaaaatcaa aaaggcgatc gagtattact atgccgagat cagtaccaag
     6781 caggcagcta aaaaggcgaa tatttccgca gaagatattg aggtgcccca gctgacggca
     6841 gacgatgcgg acgacgatgt gccgatcatc aagctgatca actcgctgct ggtacgggga
     6901 tacagcacca acgccagcga tattcacatt gaaccgttta aggatttcac acaggtgcgt
     6961 atgcgtatcg acggtcagat ggtcagctat ctgacactgg aaaaggcact gcacaactcc
     7021 attgttgccc gtatcaaaat catgagcaac ctaaacattg cggaaaaacg tatcccgcag
     7081 gacggcaact tccacgctgt gctggaggga atcgacatca gtatgcgtat ttccgttctg
     7141 ccgacgacac acggcgagaa gatcgttatg cgttatctgg tgtcggatac caagatcgac
     7201 cacgccggtc acttcggcat gaacgagtat tcctataaca tgctgatgaa gatgatgtcc
     7261 gcccccaacg gcattatcta tattacgggg ccgaccggtt ccggtaaaac gaccacgctg
     7321 tatatggtat tgcagcatct gtcccagcga ccggtaaata tttctaccat tgaggaccct
     7381 gtggaaaaag atttgcccaa tatcacgcag acttccgtta atgtgactgc cggcatgacc
     7441 tttgcagtgg gactgcgtgc cctgctgcga caggatccgg atattatcat gatcggtgaa
     7501 acccgtgatg cagaaacggc tgaaatttcc gtgcgttctg caatcaccgg acacctggta
     7561 gtttccaccc tgcataccaa cgatgccatt tcgtccattg tccgtctggc ggatatgggc
     7621 gtggcaccgt atctgatctc caactctgtg gtaggtatcg tggcacagcg tctggtgcgt
     7681 aaggtctgcc agttctgtaa atacgaagaa ccggcgacag cagatgacat cgccattgtc
     7741 ggggacggca ttcccatgat tacccgcggc aagggctgta aggtctgcaa caacacgggc
     7801 tataaaggac gtatttccat tcacgaaatg gtatatatag acaaaacgct gaaacgtatg
     7861 atcgccaaaa gtgcagacag tcaggaaatg ctggagtatg ccaagaaaac acagggcatg
     7921 cggagtctgc tggacagtgc cattgacttg gtaaaagagg gcgtgaccac gccggaagaa
     7981 gttatgaaga tcgcatatta ttccgactaa tgattacaga aaggacggta taccgccatg
     8041 accatgaatg aacttattat gaaagcacgg gagaagaaat gctctgatat tcacatcagc
     8101 gagggcatgc cggtttcgtt ccgtatccac gggcagctgc tggacagcgg cttttcattt
     8161 ccggcggcag aaacacggga aatgatcctg tccatgctga gtgatgaaca gcgggaacag
     8221 ctggaaaacg gcgacgacgt ggactttgcc attgaaacgc cggactattg ccgccagcgt
     8281 gtcaatgttt tccggcagat gaaaaaggtg gcagccacga ttcgtctgct gaacaacacc
     8341 attcccacac tggaacagct gcaaatgccg aaagtgctgc gggagttggc agaccagcca
     8401 agaggactga ttctggtgac aggtccgacg ggaagcggta aatctaccac tctgtccgcc
     8461 atgatcgact atatcaacaa gaatcgttcg gagcatatca tcaccattga ggaccctgtg
     8521 gaatatgttt acgagtcggg catgtcggtc attcaccagc gtgaaatcgg cgtggatacc
     8581 aagagctttg cagcgtccct gcgttctgcc ctccgtgaag accctgatgt cattctcgtg
     8641 ggagaaatgc gtgactacga aacgatttct gccgctgtca ccgccgcaga aaccggacac
     8701 ttggtcatgt ccactctgca caccaccggt gcggcacaga cgctggatcg tatcattgac
     8761 agctgtccgc cccacgccca gaaccagatc cggacacagc tgtccacgat tctgaagggc
     8821 gttatcacac aggctctgat cccgctggca atgggcggcg gccgtgtggc tgctacagaa
     8881 attctggtgg gaaccgatgc ggtaggcagc ctgatccgtg acaataagtg cttccagctg
     8941 gcaacggtca tgcagtccag tgcggcactt ggcatgcaca ccctgaatgc cgatctggca
     9001 cggctggtaa gagccggcac gatccgtcgg gatgatgcca ttcgggtatc caacaacaag
     9061 aatgagattc tggatctgtt aagttaagct tcgcttcggc gtgtccgtct gtatattttc
     9121 ggagaggtga gagagatgaa acgaaatcga aaaggctttt ctcttgtgga attgatcgta
     9181 gtgctggtga tcatggcaat cattgcggca ctctgtgccc cgaatatctc tgcctatgtg
     9241 caggcggcaa aggttcagaa ttatcagact gtggcgaata atctggcaga cgaattacag
     9301 acccagctgc cccagagccg gtactggaac tgggacgagg tgcagcggaa tgcgcatggc
     9361 attctgtgtg cagatgccgg aagatacgtg acactggata cagaaaaaag caccgataat
     9421 caaaaggtgt atcagatcac cggtgccagc agcgatgcca atgcggtgtt tacggtgacg
     9481 ctgacctatc atgatgcggt gaaaactagt cagaaagtgg atattgcggt ttcctgcgac
     9541 gggtacgctg cggtgcagtc ggtgcaggag tgtaatttgg tgttgaagac gaattataca
     9601 gatgcgagtg cgtatccgat gagcagcacc acgatcacca caacagaaac caaaggctgg
     9661 gggaatctgg acgatagagt caataccaaa tcgtactggg aagaatgggg aaaaacacat
     9721 cccgagaatg actttacaaa agatgcaact tggagtggct ggtggacacc ggacaccacg
     9781 atcgtcaatt tgacagacta tgtttccaac agctgtaagt tcgtgcagtt tgacatgccc
     9841 tatttttgcg gttatcatca aaccaatttg aatcctgtcg aatatgcagg aagctatgcc
     9901 tttccgtcta tgtacagtct gaatggtgca ggcgacggtg cacagtatgc agttaccagt
     9961 tatagtgacg gttccgaaag tacatgggaa aaaatctata tttacatagg cgatgacaat
    10021 aacacagaag atctgaaatt cattgataaa gatgctgacg gaaatgagat ctacgacagt
    10081 gctgcaatgt acgatcatgg ctgggtaaaa tacagtgata tttttgcaaa cgagcggggt
    10141 aattttcgaa ccgacggaaa tccggattat atcagtgctt ttcaaaataa gtacgattct
    10201 tccattactg taaatccgga cagtacattt gtaaataaca caaagataaa ataccgtgta
    10261 cttttacgaa ctaacagcgg tgaggaatcc agccgtggct ggctctatgt agaaggaaca
    10321 tatgccggaa tcggaaaaaa gtgttctccg gaaaagaaaa attttctggt gtatgcaaat
    10381 aatggttctc aactgaatga cttttcagat cgatatgaag tggataagca gagttggaat
    10441 acttccattt atatggacgt ttcgaaatgc ggatactctg tgggaaatca gttgagcatt
    10501 acaattcgca atgttgatgc cgataaactg atcgattcca tggaaaatgg aagaacggga
    10561 gatgtaagcg ttggacagaa caagtcctat gatggatatg acggcgtaaa atatggcagt
    10621 attcaacaaa gttcatttat ttacggaaaa ccggaaaacg gcgggaatta ctgggcagaa
    10681 aagccagatg catcagacaa ttctacagct agaattgtta ttgtcatgac atacagccgc
    10741 tacccttatt tgaaattagt atttcttagc agcttttgga catctggtgc aaccagtaca
    10801 gatgtagaca ttagcctgaa aatggattac tccggcggaa actcgtctga aagtacgatc
    10861 gacagttaca cccccatgct caccctcaac ggcgacagca cctacaccaa catcaccctc
    10921 gactggacga agtgcgacta tatcaccaat gcgggcgaag tgggcgtgaa gtttgtcaag
    10981 tatccgtcca cgctgaatta tgatgtgtac agcaaggatt acaccctgct ggctccggat
    11041 cagaacctga aaacctttat gatgaattca aacaatgctg tgacatcggc tgctttggcg
    11101 gataagatcg tgcacctcta ttgtaagggt gtcagtccgg aggagattca gtgcgagatc
    11161 gttgctccga aaaatacggc aacgacaacg actaccacga ctaccacacc tccgccgcct
    11221 gaaaccgaaa ccacagccaa aacgactacc agcacgacga ctgcggctac aaccaccaca
    11281 acaacgacta ccacaacaac aactacaaca acgacgacta cagcaaccac cacaacaacg
    11341 actaccacga caactaccac aacgaccgcc gcaaccacaa gcacggctgc aacaactgca
    11401 accactgcga ctgccgcaac gaccaccgcg gaaaccactg caagcagtac cgcaacggcg
    11461 actacctccg cgacagaaac tacggctgca acgactacaa cctccgccac aaccagcacg
    11521 actataagca ccactgcaac aactgcaacg accagtactt ctgcgacgga aacaacactt
    11581 attacgacag ctagtaccga tgttacaaca gaaaaaaatc aattgataat atatcatatt
    11641 tatgattcaa ctaaaaatgg ttgttatgca gaaattaaac cttcatattg tactgaaaat
    11701 ggaacacaat acaatatgaa cgtcataatg aaaaaggaag atggcagttt aatttcaaat
    11761 aatagttgtt acaacttgaa tccggaaaat gggaaaacct cgtttattgt tgcaacactt
    11821 gaaaataatg gtgctattgc gatttatcca aaattgtcat ggagtgatgc tacatatgat
    11881 gcaacaatac ctaatatttt gcaatcatat aatgtatgca gttggggtgg gtatcacgtt
    11941 gactatgatg atgttgtaaa tggtcttcac gaactctgga ttaaaaacgg agttgtttat
    12001 aaggctaatc ctgacacacc acttgctcaa atggtaaaca ctgtatatcg agaaaacagt
    12061 atcgctgctt gccttcctaa atctgttgaa gttgttacta caaccacaac aaccaccgtc
    12121 cctcaaacca ccaccacaac tacaacaaca accaccactg caccgcccat tgctgccagc
    12181 gaaaacggca aaaacgtagg tctggacgaa tccagtgcgg cgggctgcaa catcacgttc
    12241 agcgacccgc tgttcaacga cagcggcgtg tgctacttca ccattacccc caaggcgggc
    12301 tacacgctct ctgacagcta taccgtaaag gttggcacgg aggtgctgtc tgccaagaac
    12361 aactacaacg ccaagacggg gcagtggttc ttctggagca ataagatcat gacggactac
    12421 accattcatg tggagggtgt ttctccggcg gcgtaacgct cgaaataatc caaaaagaaa
    12481 aggctcattt ccggagcgta ccggagatga gcctttctgt gttcgggcgg tcatttcaga
    12541 ccgcagagct gtaagtacag atttgcaatg ggttcaccga ccaccagagc cattgccatg
    12601 ccggcggaga ggtacggccc aaaggcaaat ttggaatcgc cggtttttgc cttgtggatc
    12661 atgccgaaga tgcagccgag aatcagtccc agaaacagcc ctaccacaac acctttgaca
    12721 ccgaggaaca agccgcctgc cagcatcagt tctgcgtcgc cccgtcccat ggcacgttcg
    12781 ccggacagaa ttaccagcag gaacagcgga accgatgccg ctgcggctcc gatcaggtgg
    12841 gagagcagcg tgccgtttcc ggtgcagaca tcgtaaaccg taaccgcaac gcccagcagc
    12901 ccgatgaatc cgatcacata cagactgatc agctgggtgt ccaagtccat gaaaaagacc
    12961 acgattagag ccgaacagaa cagacatacc agagccgcat agaacggctg ttcaaagatg
    13021 ccgaaatagg caaagatcaa cacgtataag atcgcattca ggctctccac cagcgtgtag
    13081 cgaaagggga tttttgcccc gcagttgtgg cattttcccc gcagacggca ccagctgaag
    13141 atcgggatca gatcccgctt gcgaatgggt gtaccgcagg tcatgcagtg ggagttgttc
    13201 cgccagagag attcctcttt gggcagacgg tagatacaga cgttcagaaa gctgccaatc
    13261 gccgcaccaa aagcaaaaat gatgcagtac agcagtacat aacagacgta atacataggg
    13321 caaattcctc tcttttttct tgtcctattg tatcacattt tgaaaaagat gtcaagaaat
    13381 atggaaattg cgtgtacaac cgggcggctg tgtgattttc acgaaaatcg gactataccc
    13441 ttgacgaaag cggcaaaaag tagtacaata ttgtaagaag ccggtgcatt gccggttagc
    13501 atttatggga ggtattacac catgtctgaa aagtttattg ttgaaacatc tgcaagacac
    13561 gtacatctga ctgcggaagc attcgcagtg ctgttcggtg agggaaagga actgactgtc
    13621 aagaagatgc tgtcgcagcc gggtcagttt gccagtgaag aacgcgtaac gatcgttgga
    13681 ccgaagaagg aactgccgaa cgtttccatt ctgggtccgt tccggagtgc caatcaggta
    13741 gaactgtctg caacagacgc acgttcgatc ggcatcgctg ctccggttcg cgaatccggt
    13801 gatgttgcag gttccggtgc atgtaccatt gtgggaccgg caggtcagct ggaaatttct
    13861 gagggtgtga tcgtggcaaa gcgtcacatt cacttcactc cggaagacgc agcaaaggca
    13921 ggcgtaaagg ataaggacat cgtttgggta cgtgttgaaa cagacggcag aaaggcaatc
    13981 ctgggtgacg tggtttgccg tgtttccccg aactatgcaa ctgcaatgca catcgacacg
    14041 gacgaatcca atgccattgg tgcaggccgc aaccagatgg gcgagatcgt cacagtagac
    14101 tgatcacatt ttgatggaaa ggcaatcctg tactaagcat gtgcagtgtt gcctgaaaga
    14161 caaacggcgg atgctgaaat ccttttggca tccgttgttt ttttagaaag gaacaactat
    14221 atgagatcat ggaaaaaatt gacggcactg cttctcgccg gtgtgctggc tggctcggca
    14281 tttaccggct gcggcaagca gagcagcgac agcagccagc cggagtcttc tgctgccgag
    14341 gaatcctctg aaaaggaaac cactaccgcc gcggaaaaaa ccaccaccgc ggaaactacc
    14401 acagttcacg tcagccccac agaaacggtt tccaccacgt tgggcacgga actgaccgac
    14461 atcaatgcca tgctggacaa gggcagcgat accaccaaca attacaaagc caagctggat
    14521 aactttattc aggagggcga cgtggtacag tcctttacct ttatcgtgta ctccgccgac
    14581 ggttcgtcca gtctgggcga cttcaagggc ggctacggcg tttccgtcaa ggacggctgt
    14641 gccagtgcca ccgatgccgg ctggtatcag gcggcagact ttgagcagtc ccagaacagt
    14701 gcctatgcgg aattcacatg gaatgttccc gcagagatcc agacggagat cgaccccagc
    14761 ggcgaagtcc tgttcggaca ctggtggagc aacgtgcagc aggtgaagct gtccagcatt
    14821 gtgtgcacct acacccgtac caaggaagtg ccggtagacg gtaccaattc cgccagtccg
    14881 gctgcaactt tgagtttcaa cgatgaggca gccaagacag cgaagatcgc tctgtccgac
    14941 ctgatcgggg cagaggatac gcttcagaca gtcacctttg acatcagcag cactggtgca
    15001 ctgggcaagt tcaccggtgc gttcggcgtg tctgtggaca agaacgcaga ctgtgccacc
    15061 gatgctggct ggtatcagac caagaacgtc tgcgtgttca cagattcctc cagcctttcc
    15121 ctgacgtgga tcgtgccgga cgatgtcaag gagagcattg accccagcgg cgatgtgatg
    15181 ctggggtact ggtggagcga ccagcccagc attaccctga acaacatcag cgtccgctac
    15241 agcaacagca ccggaacgac caccaagacc gagccgaagt cggacgagtc ctccaagggc
    15301 ggggaagtgg cagcagtttc cggtgaaaca cctactgcgg agcaggtcaa cgcgatgacc
    15361 agcaagcaga tcgtggagaa catcaaggtg ggctggaatc tgggcaactc actggacagc
    15421 tacaacacca gctccagcga tacggaaacc ggctggggca acccgaaaac cacccagcag
    15481 atgatcgaca ctgtaaaggc tgccggcttc aatgccattc gtgtgccggt aacgtggagc
    15541 gagcacatgt ccgccgacgg caccatcgat gcggcgtgga tgaaccgcgt caaggaagtt
    15601 gtagactacg cctataacga cgggctgtat gtcattgtga atgtacacca cgatgactac
    15661 acatggctga caccctccag tgaaaaactg gaaagcaata agtccaccct gacaaacatc
    15721 tggaagcaga tctgtgccac attccagaac tacgaccacc gcctgatctt cgagggcatg
    15781 aacgaaccgc gtatgatcgg ttccgcagag gaatggaccg gcggcacaca ggaatcctat
    15841 gacgttatca acgccctgta tcaggcattt gtggacacag tccgttcttc cggtggctcc
    15901 aacaaggata gaacgctggt tgtcagcacc tatgcccagt ccgtggagaa gaacgctgtc
    15961 ggcggtctgg tggttccgaa agacgaccat gtgatcgtat ccctgcacat ctacgccccg
    16021 tggaatttct gcggtccgga cgacacccgt gccgactggg gcagcgatgc cgacaagcag
    16081 gaactggaca ccatgttcca gtatctggac gacacctttg tcagcaaggg cattccggtc
    16141 atcattgacg aaaccggctg cgtgaacaag aacgaaaaca cctccgcaag agtagcatgg
    16201 ttcagctatt atctcagcac agccaagaag cacggcatca agtgctttat ctgggacaac
    16261 aacgaaacga agaacggttt cggtctgctg aaccgcaagg actgcacatg gtattatccg
    16321 gaaatcatcg aagccattca gaatgctacc aagtaaacgg atattgtcat aatgcataaa
    16381 tcattcccca tatagatgtg caaaatgtag aatttttata aaagccagaa aaaattccgt
    16441 gtgaaacgtg caaaaaactg caaagtatgc tataataaga gaaagcactg gaaaacgggt
    16501 acgcccgctt ttcaaacaag aggaggcatt ttactatgag tcatcgaatc attaccattg
    16561 aaagagaata tgcaagcggc ggccgtgaga tcggcgaaat gatcgcagaa cggctgggca
    16621 ttccgtgtta taatcgagaa attttacaaa tggcatctga acgctgcaat gtttccatgg
    16681 attatctgga aaccgcagag gaagctgccc ccaagagttt tttgtacact ctgatgctga
    16741 cctccagccc tacacgcacc attgaggaaa acctgccgct gtctgataag gtctacatca
    16801 tcgaaaccaa catcattcgg gaactggcgg aaaaaggcga ctgtgtgatc gtgggacgct
    16861 gtgcaagcta tatcctgcgg gatctggaaa acctgttcaa cgtgttcatc tacgcggaca
    16921 ccaagtcccg ttcggaacgt gccgttcagg agtatcacgt ggacgaacgg cgggtagagg
    16981 caatgctgcg gaagatcgac aagcgtcgtg aaacattcta cagcatcaac accggcggaa
    17041 actggtacga caaggacaac taccacctct gcctgaacag cggagcactg gggctggaac
    17101 tctgtgcgga tattgtggtg gaaaccgcaa agaaaaccat ttattaagtc attcgaaaga
    17161 atagatacag gcgggacagg gaagcttctc tgtcccgctt ttttgcacgg aaatcaattt
    17221 tttcatgata gcctcccctg aaaggggagg g
//
LOCUS       DAWBGR010000035        17249 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1114_length_17249_cov_3.332732, whole genome shotgun sequence.
ACCESSION   DAWBGR010000035 DAWBGR010000000
VERSION     DAWBGR010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17249)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17249)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17249
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1114_length_17249_cov_3.332732"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    742..793
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2742..2751
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15029..15038
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16218..16227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4363 a         4266 c         3530 g         5008 t
ORIGIN      
        1 ttagcatatc gccatctact aaaacaattt tttgtggcat tttttgatta gcaagttgaa
       61 ttgcttcatt tgagtatttc gctgtcgtaa taaacaaacc tttagaagca ccttttccaa
      121 ctaatgcacc taaaaacttt tggatttctg gtctgctgac tgtagaatct agcttccatt
      181 gtttggcttg cacataaatt gtatcaaacc caaatttatc tgcttttatt gaaccgtcaa
      241 tgccagcatc gcccgatttt tttgtaacac taggttctcc atatcccatc ttctccaaca
      301 actgaacaac taatttttca aaatcataag gagacatttt taaaactttt tgcatcaagt
      361 taatagcaag actttcatta agttggcgac taatagtatc taactgttct tgaggatctt
      421 gcaaacttaa ttcaggagat tcgctatcat tgcaagtgga gttgtccata gaattagcta
      481 ttgatttttg ctctttttct cctttgaatt ggcgaaaaga ttcaaattgt gtaagatatt
      541 cgagagttat attttgtact ccattttttg cagcttgttt ccctaatgtt gtgattatat
      601 attttgatcg gcttggactt tcaataagac ctgcattctt taggtatatc tctgcccatg
      661 caattctatt gctgtatttg tattgtccac tagttaatct ttcttgtagt tcttcttctg
      721 ttatggaata ataactcaca cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnttttgta gaatgttctt tttcgtctaa taggcattcc ataacaggag
      841 caaaaaattc aaaatatttt ggaattgcca ttacttttct tccttctcaa accgcaccgt
      901 aattgcattg gcgtgtgcgg tcaggccttc cttgtttgca atcagcacaa tatcgtcctt
      961 agctgcatgc agagcgtcct tggtatagta ggtgtagctg gaacgtttgg taaagctctg
     1021 tacgcccagc ggagagaaga accgtgccgt tccgctggtg ggcagaacgt ggttcggacc
     1081 agcgtagtaa tcgcccagcg gttccgatgc atatgctccc agaaataccg aaccggcatt
     1141 gtccagtgct cccagcagtt ccatggggtt ttccataagt acttccaagt gttccggtgc
     1201 aaccatattt gccagaacag tggcttcttc acgggtattg cacagcagaa ctgcaccata
     1261 ttcattcatc gatttctcaa tgatttcctt gcgggaaaga tactgtacct gacgattgat
     1321 ttctgcttgt accttttctg ctagttctgc actggtagtt accagaattg cagaagccag
     1381 tacgtcgtgc tctgcctgtg acatcagatc tgcggcaaca aatttcgggt cagcagtttc
     1441 atctgccata accagaactt cactgggacc tgcgatcata tcaatatcta caacaccgta
     1501 cagcagcttc ttggcagttg ccacaaagat gttgcctggt ccgacaattt tatccacctt
     1561 cgggatttct tctgtaccat aagccagtgc tgcaattgcc tgtgcaccgc cggagaggaa
     1621 tacacggtct accccacaaa gtccggctgc taccagaata tccggattcg gtgtgccgtc
     1681 cttgcagggc ggtgttacca tgatgatttc cttaacgcca gcaatttttg ccggcactgc
     1741 gttcatgata acgctggagg gataagcagc cgtaccgccc ggcacgtaca gaccaacacg
     1801 ttccagaccg cggacacgct gtcccagaat tacaccattc ggcagtgcgt ggataaagcc
     1861 ttcctgtacc tgacgctggt ggaaatcgcg aacattttca atagcgttca gcatggcatc
     1921 tacaaatgcc ggatcagctt ctgtcattgc gtcattgatg acatcgcggg gcacttcgta
     1981 atattccggc aaactgccgt cgaatttcaa ggtgtaattt ttgactgcct gatcgccgcc
     2041 ctctttgact gtgcggataa tttcgctgac agtttctgta acacgctggt tggtttcacc
     2101 ggtacgcttg tccaagctgg tgaaaaatgc atcacgctgt gccagatcgc tggcgttaat
     2161 ggttttcatg gtaatcattt tgataaagcc tcctcaattt tctgaatcaa agcttcaatt
     2221 tctgtctgcc gcagctttag actaacggta ttggcaatca gacgtgcaga gatcggaacg
     2281 acatcttcta ccacttccaa gccgttttct cgaagggtaa cgccggtttc tacaatgtcc
     2341 acaatgccgt ctgccagtcc cagcaacggt gccagttcca cggaaccttc aatttttaca
     2401 atatcaacgt ccatgccttt gctttcaaaa aagcggcgtg caacatttgg atacttggtc
     2461 gcaatgcact ttacgccgaa gccgctgtaa aaatctgttc cttttttgca tgccagagca
     2521 aatttgcatc tgccaaatcc caaatcgacc agttcgaaaa atttgccctg catttcaata
     2581 atggtgtctt ttcccacaac gcccatatca caaacgccat gttctacata ggtgatgacg
     2641 tcatttgcct ttgccagaac gacttccatg ttgccgtccg gaatcggcag gatcaggcgg
     2701 cgaccttttt cccgtacagc ggtacaatca tagcccaacc gnnnnnnnnn ncgttccagc
     2761 agtccgacag tatctttttc cagtctgcct tttgtcaatg caattctaag cggctttgcc
     2821 atttattcta cctccgtaat ttcttcatct acaacaacga cttttccgat tttattttcc
     2881 cgtgcatatt cccgcaccag ttccagactg tcaaagaatg caaattcaac aacctgtccg
     2941 cggctgcgta attcctgtga aacctttgcg gctttcattt catagccagt tgctgcaaaa
     3001 atgacagcat ctgccggctt caggacaaca gggcggtcct tcatgcggac atttgccaca
     3061 gcatcgacat tgactgcaaa gccggttgcc ggaatgtcat aaccaaaatc agacagcagt
     3121 ttattgtacc gtccgccgga gagtacttct tctccgtagc cttccagata gcccttgatg
     3181 atgacaccgg tatagtagtc agcattattg accataccca gatcgacagt cagacgacct
     3241 tttccgatta cggtggaaat gtcattatac agtttcttca gatccataag gatttcatcg
     3301 attcgctgat tggaataaag ctgtgaagcc ttttcaaaca cttcttcacc gccaaacagt
     3361 gccggcagtt tcttcagtgc agcagcaact ttcacatcat ccatggtgtc cagcacgtcg
     3421 ttcaatgccg gatagttttt tgtttcaatc aaataacgga tattttcccg ttcttccggt
     3481 gtggcatgca gttcccgcat cagttcctta aatacaccgg catcacccag ttccaaagag
     3541 aagctcacat ggtcactgct gaacgaatcc agtgcctcaa tggctgtggt aatcatttcc
     3601 acatctgcca gataggaaga tgaacccagc agttcaatac ctgcctgtgt gatttcgtcg
     3661 cttctgcctt tgagtgcagg ttcaaagcga tacactgact gagtatagca cagtcgcagc
     3721 ggtaataatg catctcgcag acgggttgct gtgacgcgtg caatcggcat ggtagattcc
     3781 ggacggagta ccagcaagcg tcccttgctg tcggtcagct tatacagtcg ttcctgcgga
     3841 tagtgaccgg attctgactg gaacacatcg aaaaattcca atcccggtgt agtaagttcc
     3901 gaatagcctt tgctgtgaaa caagtcatgt agattctgct cgatttcacg gcgcacataa
     3961 cattcttcaa ataaaaaatc ttttgtcccc tcaggggtaa tcaaatcata ttttctcata
     4021 cagctctcct aatgctttag tgtgctacta taatagcata atatcacata aaacgaataa
     4081 agtcaagaca aatttcttaa aacagcgaca tctgactgga tttcggtaaa tcgcccatag
     4141 cattcaaccg ttccaacgtt tcaattattg tttttgatgc accactttgt gtctgaaact
     4201 cttcaataga aatgaaatcc cgcttttgtg ctgtttcata caagttctgt gcagctgaaa
     4261 taccaatgcc cggaatcgca ctgaacggaa tccggatttt atgatcttct accagatact
     4321 ttgtagcatg agacttgtag agatcaatag gcaggaattc gcagccgcgg ctaagcatct
     4381 cattgacaat cagtagcgta tccagcaaat cagattcttt tttggaacgg tcattcccct
     4441 tttccttgag ttcttcgatt tttgcccgaa cagcttcctt gccctgcaac gccagaattg
     4501 catcgaaatc atcaccgcga gtactgaaat acaccgcata atacgccaat gggtgataca
     4561 gcttgaacca acccagttta atggcagcag taacatacgc tgctgcatgg gctttcggga
     4621 acatgtactt gattttcaga cagctgtcaa tataccattg cggcacattg tgatctttca
     4681 gcatttgtac acgttccggt gtaaaagtct tgggggcttt tccctttcgg gtgtcttcca
     4741 tgatttggaa tgcctgtttc ggctcaacgc ctttgtgcag cagataggtc ataatgctgt
     4801 cacgggtacc gattacgtca gaaatggtac aggtgccgtt atcgatcaag tctttggcat
     4861 ttccaagcca tacgtcagta ccgtgggaaa gtccgctgac ttgcaggaag tcgctgaatt
     4921 ttttcggttt ggagtccagc agcatctgaa tggtgaagcc ggttcccatt tccggaatac
     4981 caaggcttcc cgtctggcag tagatttctt ctggtgtaac gcccagaaca gaagcatcgg
     5041 tacacatttg aatgaccttg ggatctgttg tgggaacatt gtgaatcaag ataccagtgg
     5101 catcttccaa gtgcttatac atggtcggta cgacatgccc cagttcgtcc agtttcaaaa
     5161 tggtatcgtg gagcgaatgg aagtcgaagt gtgttgtcag aatttcagat tcggcatcgt
     5221 ccgcagggtg ctgtaccggc gtgaagtcat atacatcata atcgctgggg acaacaacca
     5281 tgccgccggg gtgctgacca gttgttcgtt tgatacccgt acagccgatc gacagtcggt
     5341 tgatctcagt aggactatac tgcaatccct tttcttccag atattttttc acgtaaccat
     5401 aggctgtttt gtcggcgaca gcggaaatgg ttcctgcttt gaacacgtta tctcgtccga
     5461 aaagttcttc cgtgtaacgg tgagcggatg attggtattc actggagaag ttcaagtcaa
     5521 tatccggagc cttgtcgccg tcgaatccca agaatgtttc gaaaggaata tcatgtccgt
     5581 cccgattcat ttccgtaccg cattccggac agttttttgg aggaaggtca aagccggaac
     5641 caatgctgcc gtctgtgata aactcgctat gtttacactt ggggcagacg tagtgtggtg
     5701 ccaacgggtt gacttcggaa attcctgcca tagatgccac aaaagaggag ccaacagaac
     5761 cacgggaacc aacttgatac ccatggtcta cgctatccca aaccaatttc tgtgcaatga
     5821 tatacagcac agaaaaacca tgtttgatga tagaggagag ttcgcggttc agtcgctttt
     5881 ctaccagttc cggcaatggg tcaccataga tttcctttgc cttagtattg gtgattcgca
     5941 ccaaatcttc ctcggcaccg tcaatggtcg gcgtaaaggt accgttcggg aacggctgaa
     6001 tgtgttcaat ttgatctgca atggcattgg tgttggtaac aacaatttcg tatgcctttt
     6061 cttcaccaag ataggagaat tcttccagca tttcttccgt ggtacgcaga tacagcggcg
     6121 gctgaaattt tgcatcgtca tagccttgtc cggcttgcag gatctcgcgg taaatggcat
     6181 cttccttgtc catgaagtgc acgtcacagg ttgcacaaac cggaattccc agttcatcac
     6241 ccagtgcaac aattttgcgg ttgtattcct gcaattgttc catattggca gcagtaccgt
     6301 ttcgaatcat gaaagcatta ttccctgccg gctggatttc cagatagtca tagaactttg
     6361 ccagtttcac aatttcctca tgcggtttct cttccacgaa tgctcggaag agttcgccgg
     6421 cttcgcaggc actgccgaaa atcaagccct cccgatactg catcaacact gatttgggga
     6481 tcaatggctt tcggtaaaaa cattcgatat gcccatagga aatcaacttg tacaaatttt
     6541 ttaagccgat attatttcgt accagaatga tctggtgaaa cgatttcagt ttttttacat
     6601 cgatttcctg tgttttggtg ttcagatcag aaagcattgt ggcacccttt gtctgcatca
     6661 gacggctgac caattctttg taaatttttg ccagaatctg tgcatcgtct gatgcccgat
     6721 gatgatcgaa ctttcccaat ttcagagcct ttgcaacacc atccagcttg tggcgtgcca
     6781 tagtaggcag catgctcttg cagagtacca gtgtatccag tgttgcgaac tcaaatgtga
     6841 tatgatttct cttgcagaca ttgcggacaa aagaagtatc gaaattggcg ttgtgtgctg
     6901 ccaaaaccgg attttcaccg cagaattcaa taaacttcct cattgcttct acttctttgg
     6961 gagcatcttc taccatggaa tccgtaatac ctgtcagttc cacgatcttt gccggaatcg
     7021 gtcgttccgg atttaccatg gtgttgaatg tttccaccac ctgcatgcca cgcatacgaa
     7081 ctgcaccaat ttctgtcagg cggtcattgg ctggactcaa gccggttgtt tccacatcaa
     7141 aaacaatgat ttcgtcctgc agtgttcggt cttctggatt atcaactacc agtagacgat
     7201 tcaagtcatt gactacatag gcttcgcagc cgaaaatcac tttgaaattc ttgtccttaa
     7261 agccacgcca cgcattcatg gcatccggaa atgcctgtgc aataccatgg tctgtgattg
     7321 caattgcagg atgtccccat tcatgggcac gttctaccag tttgccacag ggcgtaactg
     7381 catccatttg ggacatgttg gtatggcaat gcaattctac acgtttcttg ggggcattgt
     7441 ccattttccg ctgtcgtttt accacaacaa tagattccgg tttcatgaca tcttctcttg
     7501 caaattcatc atagccgatc cgtccgatca tcaataatgt acttcccttt ttgatttcat
     7561 gaaacggcag cttctcaatt ttttctgtgg aatcgaaaat cttgaccaat accgagtaag
     7621 tgccgtctgt gatctgatag gtcaaaatgg ttcgctctcc ccgtacttcg cgggaatcca
     7681 ttgcaaagac atcacccatg attaccacac gttgattctg tacttctaca gcttcccgaa
     7741 tcggcatagg aatatcccga atcggtctgc ctttgatcag gatagcactt ccaggaacga
     7801 tgcccggaat atcctcgttc atagtaacgc tttgtgcaat tggcttttcc ttatcgctgt
     7861 gagttactgc cggagcaggt gctgtagaag tttgcagttg agcttcatgt ttgggtaatt
     7921 cctgttctgc cttggagatc atttcatgga aagcagcttc actgacactg gtttcccctt
     7981 caaataccac atgaaacggc gtagagaaca ttttttccag caaaccggaa aagaaatctg
     8041 ctacgtgata ctttttcaaa atatcatagc cgccgttgtg cagttggatt cgaatggtat
     8101 tatcttcccg tgtaatgtct gctccgtcaa caaaaccgtt gacaacggtc atttcccgtt
     8161 tcagaaacat gaccagatcc ggaaaatatg ccatagaaaa caattctggg gcataccgtg
     8221 gatgcagacg aacagcatga atttccagcc gaatttccag ttcgtgttcc agtgcgacca
     8281 gatcctgtgc cggtaccaga tggggaaatg tgatataaaa cgaaattttc gtcaggtttt
     8341 cggaatatgt cagccgattc aaaataccgt ctttgatgga ttctggcata atcgggacat
     8401 attcttccag aaactctgcg aaattcaaat ccatatgttc cactcctaca gtttctcaat
     8461 ttcagccatc agttccggaa caatgtcctg ttccgcgacc tttcgtaaaa ctgttccctt
     8521 gcggaacagc attgcacagc cgtcaccacc ggcaattccg atgtctgctt ctttggcttc
     8581 tccgggacca tttacaacgc agcccatgac agctacttta atgtctttct ctacatgttc
     8641 cagagctgct tctacttctt ttgctgtttt taccagatca attcgtgtcc ggccacaggt
     8701 aggacaggat acgatttgcg gtccgccacg cttcccaata catttcaaaa tatctcttgc
     8761 cgcacgaatt tccttgacag ggtcttctgt cagggatacc cgaatggttt cgccaatgcc
     8821 gtcgtgaatc aacgagccaa tgccaattgc agatttcagc atgcccatac gctctgttcc
     8881 agcttctgta acgcccagat gcagcgggta attgcactgc tgtgcacata gacgataagc
     8941 atcgatcatg gtattaacat cggaagattt catggaaatg acaatatcat caaacccaca
     9001 ttcttccagc atatgtacat gccccattgc actttctacc aacgcttccg ctgtcggagc
     9061 accgtacttt gcaaggattt ctttttccaa tgagccagag tttacaccaa tgcgaatagg
     9121 gatctgtcgt tcctgacagg cgtggacgac ttcctgaaca cggctcatgc cgccaatatt
     9181 gccggggttg atgcgaatct tgtcgattcc tgcttcagca gcttccaatg ccagtcggta
     9241 atcgaaatga atatccgcca ccaacggaat cgagattttt tccttgattg ccggaatcaa
     9301 tgcaatggct tcatgatttg gaatcgctgc ccgtacaatt tcacagcctg cctgttccaa
     9361 tgccactgcc tgtgcaacac tgcctgcaat atcatcggaa cgagtgttca gcatggactg
     9421 aatgtagatg tgttttccgt ccagtataca attgccgact tttaccggct ttatttctct
     9481 catgattgtc actccctaat ttttgaagaa tcgccctata tcctgtactg ttaccagaat
     9541 catcagcaga aacagtgctg ccataccaat aaaatgaacc atagcttccc gattttttga
     9601 aattggtttt cgtcgaatcg cttcaatcaa cagaaacaca aaccgactgc catccagtcc
     9661 cggaatcggc aaaaggttga atacacctac atttactgta atgaaaattg tcaaattcat
     9721 gacagactgc acccgttcca gcagattttc tccggtagat gctgcctgtt caatgacact
     9781 gacagttccg acaggaccag aaaggtcttg cagactatat tttccgctga ccagttcaat
     9841 caatgacatc caaatcagct tggcagttgc cactgtgtct ttggcggcat aggtgatgac
     9901 atttaccgga tttttagatt ttccctccac agaaaaatcc agcagaccac ctgtgttatc
     9961 attatggaac gttacatttt caatggtaac acgggaaccg tcacgcttta cagtaatgtc
    10021 ataggtgtct gtatctgttg tttgcagttt gtaaatcaga tcagttgtgg aaaaaatatg
    10081 gctgccgtcg attgcaacaa ttttatcgcc gatttgcagg ccggatcgtt cactttccga
    10141 tgcagttaca gtatttccgg cagaattggt cgtttccgca aattttgcga ttgtcaaaga
    10201 cggcaccttc tgtgctgtgc acagcagaat tgcaatcagc agaaatccca gaaccagatt
    10261 cataaatgca ccggcaagta ctaccgtcat tctacgccag actgcttttt tttcaaaagc
    10321 gttaggattg tcactggact gatcctcgcc ttccatggca cagaatccac cgattggaaa
    10381 caaccggaga gaatattctg tttcgccttt ttgaaaatga atcagtttgg gtcccatgcc
    10441 aattgcaaat tgattgactt gaatgtggtt cagctttgca acgataaaat gtccaaattc
    10501 gtgaatgccg atgattaaac cgaaaatgag aattgcaatg aggatcgtga taatatgctg
    10561 catatgcagg tcctttctaa ggaaccactg caaacagatt ttgtgtaaaa tcgacagttg
    10621 aattttatgc agcggttcta taattgtaaa tcgttatgcg atagtatttc gtacaaattc
    10681 ccgagccttt gtgtcagccc gcataacatc ctcataggtt tctatcttgg ttgccggaat
    10741 catttccatt gccttttgtg ccaatgcacc gatgtccaga aaacgaattt ttccggacag
    10801 gaatgcagca actgctacct catttgcccc gtttacaata caaggtgctg tgccaccggt
    10861 tgtaattgct gcaattgccg ttttcagaca ggagaatgtt tccatatccg gtttttcaaa
    10921 agtcaatgtt ccgtaatcag tcagtgacag atgttttgtt ggacaaggca gccgttccgg
    10981 atacaatagt gcatactgaa ttggaatttt catatccggc acgcccattt gtgcaatgac
    11041 tgaacaatcc tgatattcga ctgcggaatg caccacactc tgccggtgta caacaatttc
    11101 aatctgttcc ggtgtcaggt caaagagcca cttagcttca atgaattcta gtcccttatt
    11161 catcagtgtt gcagagtcaa tggtaatttt attgcccatg ctccagttcg ggtgatttaa
    11221 ggcatctgct acagtgacat gctccagttc ctgacgagtt tttccgaaga acggtccgcc
    11281 ggaagccgtc agaatgattt tgtgcagctg ttcccgccga ttgccctgta aacattggaa
    11341 aatggcagaa tgttcgctgt ctaccggata aattcgaacg cctttttttg ctgcctgttc
    11401 cataaccaga gagccgccgg caaccagcgt ttccttattt gccaacgcaa tgtccaaacc
    11461 ggcagaaatc gcggtcagtg ttggcagcag cccaaccatg cctaccacag aattgatgag
    11521 aatatctgca tttttatctg acgcaatgtc acatagcccc tccataccgc agagcacctg
    11581 tactggcaaa tctgccagac gctgtttcag ttctgcgtat ttttcagttt cgaagatgca
    11641 gacctttttc gggagaaact ctcgtgcttg ctgctccaat gcatcaacat ttcgattggc
    11701 agccaatgcg taaatctgca tttcgtggga tctggcaact tccaaagact gtgttccgat
    11761 agaacctgtc gatccgagaa tcgagattgc ctttttcatt ttatcattct cctttttgat
    11821 ccatgtgccc ataaacacaa cacgatgcaa agcaaagggc ctcacatcgt gtggtttgtg
    11881 catgaaccca tgcaattatc caaagaacac cggatacatc atttccagtg cataaaatgc
    11941 cggaactgta aacaaaacgc tgtcaaaacg atccatgaca ccgccgtgtc cgggcatgat
    12001 atttccgaag tctttgattc cctgctgacg cttcagtaca gatgcactca aatctcccag
    12061 tacgctgatt acagcacaaa caatgccaat aaaaatcagc cagagatagt gtacatgtgc
    12121 gtgtgcgatc aagctgtaaa tcaaggcaaa aatcaccatt accacgacat ccgtcacaat
    12181 cccaccgaaa aagccttcta cagttttctt cgggctgatt tccggacaaa gcttatgctt
    12241 tcccagaaat gttccggcaa aataggcacc agtatcggca atccatgcag aagacagtgc
    12301 cagaataacg cttgccagac caaattggct gatatgcagc aaagtattca gcatgctcaa
    12361 agaatgggaa accaaaagtg taattgcggc aataaacagg gtattctgat atgtgatatg
    12421 cttatgcagc aaaaccattt ctgcaaacat cgcaacgata caaaggaatg tcaatgcgat
    12481 tgtcgcagaa gtgtttccgt aatactgtaa gaacggcagt gcagcaccat acagaaaccc
    12541 ggcaccgata gaaatccggc aatgcaggca cttccctgca cgaaacagtt caaatagtgc
    12601 aatgcaaatc aaagccgcaa ttgccaatgg aaatacgaac gtcttatgca gaatcaaaat
    12661 caggattgcc agaatgccgc caactacgcc agagataatc cgaactttca tcgtcaaatt
    12721 cctccaaatc gtctgccacg ttgtgcatag tctgccaaag ccttattcaa ttcgttgcgg
    12781 ttgaagtctg gccacaaaac atttgtaaag taatattctg catatgcaca ttgccagatg
    12841 agatagttgg acagccgcag ttctccgctg gggcgaatca acagatccac atccggaagc
    12901 cccgcggtat acagatgctc agaaatggtt tgttctgtga ttgcttccgg tgcaagtttt
    12961 cctgcctgta cttctttggc aatctcttgt gcagcatgtt tgatttcatc tcgtccgccg
    13021 taattgattg caagaatcag attcatcttt tcgaaatctc tggaatcatg ttccagttct
    13081 tccatacgct tttgcaatcg atcctgcaaa cggcttcggt caccgacaaa aatcatacga
    13141 acgcgtcttt ccagatactg atctgcttca cagagataat cccagaacaa atccataatt
    13201 gcatttactt catcttgtgg acgtttccag ttttctgttg agaaaacata aaaagatgca
    13261 tttcgcagac caatatcccg acaatacagg gacaggtctt tgaaattttt tgctccttcc
    13321 cgatgtccaa actttcgtgg cataccacgt ttggttgccc accgtccgtt tccgtccatg
    13381 atgaagccga catgctgcgg cagctgttcc ggaagaacaa ttgttcctgt ttcatctttt
    13441 ggtgcatttt ttttgttgaa aaatggcaga gccatatcgt tcatctccta ttctgctgca
    13501 ggttttcgag tcaatgcaat cagatgctca tgatctcctg atcctttgca gttaccattt
    13561 tgtcgatttc tgcaacatac ttatctgtca gatcctgaac ctgcttttcg ccgtccttga
    13621 cctcgtcctc tgtcagttca gaagcctttt tctttgcttt cagcttttca attgcttcac
    13681 gacgaacatt ccggacagca accttggttt cttcgcccaa tttcttaatg tctttgcaca
    13741 gttccttacg gcggtcttct gtcagctgcg gaaataccag acgcagaact ttgccgtcgt
    13801 tggtcggtgt gattcctaca tcagaagaca gaatagcctt ttcaattgcc ttcagagaag
    13861 tgacgtccca cggctgaatt accagaattc gagcctcgga aacagatacg gcagccattt
    13921 gctgaatcgg agtcggcaca ccataatagt ctacctgtac cttatccaga acagccggat
    13981 ttgcacgacc agcacgaatg gttgtaaatt catgtgccag tctggcatta gactttttca
    14041 ttttttcttc ggtttctttc atcaattctt tcatagtact tccccgatta acggggctcc
    14101 tttcaattca ttttatgcaa acaaaattgt ttttatactt ctgtggcaat cgggtctact
    14161 tcggacacaa cggtacctat atcttcgccc atcactgcat cgtagatatt ttccggtcga
    14221 ttcagatcaa ataccagcat cggcattttg ttatcacggc acatagatgc agcggtgctg
    14281 tccataacct gcagtgcact gccaaggaca tcgctgaatg tcagacggga gtagcgtact
    14341 gcatcatcgt acttgtgcgg atccttgtca taaacgccgt ctaccatcgt tgctttcagc
    14401 agaatatctg cttcaatttc caccgcacgc agagttgcag cagtatctgt ggagaagaac
    14461 gggcttccgg taccgcagcc gaaaattaca actctgccct ttttcaggtg cgaaatagca
    14521 cgattgcgaa tatatggttc tgcgatctgc tgcatgctga ttgcagtctg cacgcgaact
    14581 tccaatccca gcgattccag catatctgca actgccaatg cattcatagt agttgccagc
    14641 ataccaatgt ggtctgctct ggtacgatcc atattttcac tggaacgtcc acgccagaag
    14701 ttgccgccgc cgactacgat ccccatttgt acgccagcat tcacgcactt ttgaatgctc
    14761 ttacaaattg ggagcatcgt tgcagtgtcc aatccggttt ttttcggacc agcaagagct
    14821 tcaccgctta atttcagaag aactcgatga tacttaggtt ccatgtttct aaattccttt
    14881 cgtttgtttc tcgattttca gaccttattt ggaatccgac aaaatgcctt ccattctgga
    14941 ggacaatcta tatgtttatt ttacactatt tttcgggaaa agtaaagtga tttctgcaaa
    15001 aaaataaaaa aagagcatga caattgccnn nnnnnnnnca taatcgcctt gtgaaaaaat
    15061 attcttactt gatcatgctg gcaatttctt ctgcaaagtt gtcagccttc ttttcgatgc
    15121 cctcgccgcg ttcaaaacga acgaaatcgg tgattgcgat tgtagtgcct gcagcctttg
    15181 caacgccatc tacatagctc tgaacagttt ccttgttttc ctttacgaaa gcctgttcca
    15241 gcaggcagtt ttcaccgtag tacttgccaa ccttaccagc tgcaatcttt tcctttacct
    15301 gttccggctt atttgccatc ttcggatctt ctgccatctg agccagaatg atcttctttt
    15361 cttcgtccag aacagctgcc ggaacatctt cacgcttcag atacggcgga ttcattgctg
    15421 caacctgcag tgcacagtcc tttgcagctg ccagaatttc cggaattgca ccagccggag
    15481 attctgcacg aaccataacg ccgatcttgc cgccgccgtg gatatacggt accagaatac
    15541 cttccatgcg aacgaagcgg cgaatctgca gattttcacg aatcttcagg aacaattcct
    15601 gaagtgcttc gtcaacagtc attgtgccgc cggaaacagt agttgccttc agagcttcta
    15661 cgtcagccgg attcttttca ataatggtat cagcaacctg atttacaaat gccttgaatt
    15721 catcggtatt tgcaacaaag tcagtttcag agtttacttc aacaacacag cctacagaat
    15781 tgtctgcatt tacctttgca actaccatac cttctgcggc aacacgacca gccttctttg
    15841 cctgtgtagc cagtcccttt tcacgcagca gttcaattgc cttttctaca tcaccgtcag
    15901 cttctgtcaa agccttcttg cagtccatca taccaacacc ggtagacttc cgcagttcgt
    15961 tgacatcttt tgcagtaaaa ttagccattg gatcttcctc ctaattctta tttcaaacga
    16021 tttcctcaaa aaacccgaat agactgctca tgtcaagcat attcgggttt ttgatctaac
    16081 agatatttca gtttcgcttc tgcttattca gcgtcttctt cagcagcctt tgcttctgca
    16141 gcttccgcag cggcagcatc agcaccctga cgaccttcga taattgcatc agccataacg
    16201 ccggcaatca gctttacnnn nnnnnnncat cttggaaacg atcagtttta cagcacggat
    16261 tgcatcgtcg ttacccggga taacataatc gatttcgtcc ggatcacagt tggtatcaac
    16321 gatagctacg atcggaatgt tcagcttctt agcttctgca actgcaatgc gttccttacg
    16381 cgggtcaacg ataaacagtg cacccggcag cttcttcatt tccttgatac cgcccaggaa
    16441 tttctccagc ttttcaattt ccagctgcag cttaccaact tccttcttcg gcaacagatc
    16501 gaatgtaccg tctgtttcca tttcgtgaag ctgttccaga cgcttgattc ttctctgaat
    16561 ggtagagaag tttgtcatca taccgcccag ccaacgtgca tttacatagt aagcaccagc
    16621 acgggtagct tcttccttaa tggaatcgcc ggcctgcttc ttggtaccaa cgaacagtac
    16681 agactcgccc tgtgcggaca gatcgcggat gaacatgtaa gcttcatcca gctttcttac
    16741 ggtcttctgc aaatcgatga tgtagatgcc gttacgttca gtgaaaatgt acggagccat
    16801 tttcgggttc catcttctgg tctgatgacc aaagtgtacg ccagcttcca agagctgctt
    16861 catagatact acgcccattg tgtagtcctc ctaaataaaa tggttttgct tcctccgcct
    16921 gctttttcgg ctgcacaact ctgcatttct gcaaagcacc cgtgaaaacc aaagcagacg
    16981 tgtgaactgc cttattatta taccaaaaac cgagccaatt ggtcaagtat ttctatgtag
    17041 aatatttttg ttgatttaag ctcgcatttg tacaagttgt acaaatgctg ggattcccta
    17101 actccaccaa aatagcatct tttccaacca atcggatttg ccgaaaaccg atgatgcaat
    17161 tgtcacgcgg ccccagaatt ccgaaaaagc gtggtctgcc atacaaaatc aatgctgtga
    17221 cagttgcaga atttgcgtcc atttggaga
//
LOCUS       DAWBGR010000036        16861 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1136_length_16861_cov_5.161311, whole genome shotgun sequence.
ACCESSION   DAWBGR010000036 DAWBGR010000000
VERSION     DAWBGR010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16861)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16861)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16861
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1136_length_16861_cov_5.161311"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4322 a         4009 c         4822 g         3708 t
ORIGIN      
        1 gtgaatacag caagtattca ctccgctttt tgtttcatct gacgaaaaat ttgacaacgc
       61 atctgtcgca caatctttgt gcggtattgc cttaacacta tgcagatatg caggcggaca
      121 gaaatgttcg cctgttcttg tatataaggc actgcgaagt gcattttctc atgcgtacag
      181 aaaggactac ccatgaaaca atacttggaa atcggaaaga tcgtaaacgt ccacgggctc
      241 cgcggcgacg tgaaagtggt gccgtggtgc gatgagccgg aattcctgtg cgaattcgac
      301 accctgtatc tgggcaaggc acagaagccg gtgagcgtga cggctgcccg tctgcaaaag
      361 ggcaacgtat tgctgcatct ggagggtgtg gacaccgtgg aggcggcgga acagctgcgg
      421 aatcaggtgc tctatatgga ccgcgacgag gtggaactgg aagaaggcgt gtacttcatt
      481 caggatctga tcgggctgga ggtgctggac gcagacaccg gcaaaagcta cggcaagctg
      541 tctgatgtga tgcagaccgg tgccaatgat gtgtacgaga tcaaagacga aaccggaaaa
      601 accgtgctga tccctgcgat tccggacgtg gtgcaggaaa ctgacctcga cggaggcgtg
      661 atgcggatcc gaccgctgga gggcttgttc gatgcagatt gagatcgcca ctttgtttcc
      721 ggaaatgtgc gaggcggtgc tgtcggagag cattatcggc agagccagag ccgccggtca
      781 tatttccgtc cgctgctgga atattcggga gtatacgctg gacaagcacc gccgtgtaga
      841 cgatgtgccc tacggcggcg gcaggggcat ggtcatgcag gcagatccca tttaccgctg
      901 ctatgatgcc gtacagcagg acatcggtgc caagccccac gtgatctaca tgtcccccaa
      961 gggcagggtg ctggatcagc agcgggcatt ggaattgtcc gtgatgccgc atatttttgt
     1021 actttgcggg cattatgaag gtgtagacca acggattctg gacgagatcg tagacgagga
     1081 gatttctatc ggggactatg tgctcaccgg cggggaactg ccggcactgg tgctggtgga
     1141 tacggttgca cgaatggtac ccggggtgct gccggaaaat ctctgcttcg aggacgaaag
     1201 ccacttcggc ggtctgctgg aatatcccca gtatacccgt ccggcggtgt ggcatgatcg
     1261 ggaagtgccg ccggtgctgc tcaccggaca ccacaaaaac atcgcagaat ggcggcaggc
     1321 acagagtctg gaggaaacca aaaccaaacg ccctgacctt tatgcaagct atttggacaa
     1381 aaaatagcag ttttctttgt gggatttgaa taaaatatgc aggttcacca atttgaaaaa
     1441 atcaggattt ggcattgttg tcatgtttca caaagattga aagcaaaagg gagaatggtt
     1501 ctgtcaacac gtagaaaatt ccggaaaaaa cgcaaaatat tgcaaaaagc acgttgacgt
     1561 tctccggaaa aaacggtata atgatagcat agcagagatg aggagtgcac aaatggttcc
     1621 tatcagaagt ggggatcgcc aagcgtcttc aaatcagaga agatacgaaa tgtgttgttt
     1681 cgtgcttcag taagtatggt gtgtgaacgc acatgcatga tcataaggat gggagagttt
     1741 atcatgtttg taaaagaagt tactgtacag aatcaggttg gtctgcacgc aagaccggct
     1801 acatttttca ttcagcgtgc caacgagttc aagtcctcga tctggatcga aaaggaagaa
     1861 cgccgtgtaa acgcaaagag cctgctgggt atcctttctc tgggtatcgt tggcggtacc
     1921 gacatcaaga tcattgcaga cggttctgac gaacaggttg cagtgaactc tctggtagaa
     1981 ctggtagaaa gcggattcag cgactaatta ccgcagtgtc tgtgacaaaa gagaacctca
     2041 cgggggaaag gtccgccgtg aggttttttg ttttcggaca gcaacgtgca aggtttgtta
     2101 aggcaatacc gcacaaagtc gccagatgct ctttgtgcgg tattgtctta actgccgctt
     2161 agcccgtaac gtaccatgtt ctcgcagaag atgccgtagc cgcgggtggg atcgtccaca
     2221 aagacatgtg tcacggctcc caccagcttt ccgttctgga gaatggggct gccgctcatg
     2281 ccctgcacaa tgccgccggt aacttccagc agttccggat cggtgatgca gaccgtcatg
     2341 ttgcgggtgc tgtctgtgcc gctgtagtcg atttcctcca gcgtgatgtc ataggctttc
     2401 ggcgtgtcgc cggaaacggt acagaggatc tgtgcggctc cggtctggat ctcctgcttc
     2461 agtgccatgg gaatggcttc ttcggcggaa ggattgtgat acagcgttcc gaaaacgccg
     2521 cagcggttgt tgcacagaag ttcccccagt ggggcatcat cggaaaagct gccctgcagc
     2581 atgccggcac agccggaaac gcctttggtc acctgtgaaa tggtcaccgc ggcggcttct
     2641 cccgaaccaa agggcagaat gtcaccggtg tctgcatcgc agatcgggtg tcccagtccg
     2701 ccgaagctgc cggattccgg ctcataaaaa gtaatggtac caatgccggc actgctgtcc
     2761 cgcacccaga tgccggcttc atagcggttg tcggcggcgg cgtaggcggg agtgagggtg
     2821 acggtgcagg tttcaccgct gcgggtgagg gtgacagtta ctggcgtgcc gtcggattcc
     2881 gcaatggctt cctgtaggga ctggttggag tcgatcgccg tgccgtctac agattggatc
     2941 aggtcgccgg tctgaatgcc ggcttcctgt gcgggacaac gctgccccga tgcagtttcg
     3001 attcgttcca tgcctaccac catggcaccg tccattcgca gcttgatgcc aaagggctcg
     3061 ccgcagggca ccagcatggg cgtgtcgatc tcctgtattt cgacatcttt aatgggaatg
     3121 atcccgaaaa gccggagcga ggtggtgtct gccgcggacg atgccgtgac ggcagcctgt
     3181 acggtatgtg cagtgggcac ggcggcaatg tgcagagccg tgtctacttc cagccggctg
     3241 ccggtctgga cataatagct gtccggcagc tgccacgcat aataggcggc tgtgccgtaa
     3301 agctgtagca gcaccgcagc acccagtgcc gccgtgcatt tgatccatcg tatcatattg
     3361 gaaaagacct gcctttcttt ccgaaccggc atgcctgccg ttcgaagtct tttgttttag
     3421 aggtgttttt ttggtgcggt gtaacagcga agctggtgga ggggttcttt tgtaaaattt
     3481 tcccgtgaca caacaccaaa aacaatcccg cgggaacgac gttcgcccgt aatcttatcc
     3541 tatgtaaacc ggcaacgctt atcagcgaca atgcaaggaa aaaatcctga cattccaacg
     3601 attccaaaaa tttgaaaaaa atgaggtgta tcccatgcat accaaaccaa cgaaaaacca
     3661 acccattccc accgccgccc agctgcggaa acgccgtgcc cacactgtct gctgctggat
     3721 cctgcgggga ctgctggtgc tgacgacgat tctgttcacg tggatcacct gctggggctg
     3781 cgggctgggg tggatcagcc gtgcccgtgc cggcagcaac tggccgatag aattcgccgg
     3841 ctacgggcag atgctgcttg tcggctccgg tctgctgaca ctggggacag tgcttgtcct
     3901 gctgtgccgg aagaactggc tcaactgggc agctgcaggc agtgctactg ccggcgtaac
     3961 gctggcgatg ctggcactgt accgcgtgac cgcctacgcc tctgagcatt cgttctacag
     4021 ccggctcatg gaaatgcctg ccgcgacact gtaccggctc cagctgctgc cggtgctggt
     4081 gccctatgtc tgtgtggttg cgttgggact gctgcaattc ttctccgcgg aagctgtccg
     4141 gcggcggcag gagaaaaaac gacaggattc ggcaaaggct ccgtcggtgc tttaaggaaa
     4201 cactgaagga aacactgtac aaagcttgtg cagaagatgc cctgtttctg tttttgcagg
     4261 tgggaaaaac tgtcatattc gcgtgaagga catgtgcttt cgcatgtttc cctaaaaaaa
     4321 tttctatttt gacagatttc agtcttgcag ttccccatag aatgtggtaa aattataggg
     4381 tagaacaaaa aaacaggagg taacaaaatg gacaacaaaa aaataaaagt cctgatcgga
     4441 gatgacagtg cagaatatgg catcacctgt gccaatgaac tgcggtcgct cggtctttac
     4501 gtcgtgacgc ggcgaaaaga cgggaaagtc attctggacg cgatccggaa tgacacaccg
     4561 gacgtggttg tgatggacgc tgtcctgccg ggtatggatg ccatagagat ccttgagaaa
     4621 tctcagacat tcgaaaccgc accggctttc atcatcacat cttcttatga aaatgcgttt
     4681 atcgagcgtc aggtcatgga aaagggtgcg gcgtacttca tgctgaagcc tttccacatt
     4741 tccactctgg gcagccgcat caccgatctg gctgccaagg gcatgccgaa cccgaagaac
     4801 tctgccaacc gcaatatgga ggtagtggtg acagagatga ttcatcagct gggcgtgcct
     4861 gcacacatta agggctatca ctatctgcgt gccgcaattc tggcttcgat cgagaatcag
     4921 gcactgctgg aaagcgtgac aaagctgctc tatcccacag tggcaatgca gttcgacacc
     4981 acgtcttccc gcgtggaacg tgccattcgc cacgcgatcg aaatcgcatg ggatcggggc
     5041 gatctggata cgattaactc gttcttcggc tatactgtga acaactgcaa gggcaaaccc
     5101 accaattccg aattcattgc cctgctgaca gacaagctgc ggctgcaata cggcagaagc
     5161 accgtccgtc atgcctaagg caatactgca caaagcctgt ccgacggctc aatacagcgg
     5221 gattctgtat atttttgtat gttatcgaaa aaagttcaac ttctttgtgc attttcttct
     5281 tgcagtctgc catatactgt ggtaaaataa aaagagcatg catttgttcc ggcgggcggt
     5341 ctgtccgcag acagcatgtt attatcttat gcgaaaggat ctgattttat gactgacgga
     5401 aaaatgtttt tcaagaagat ggacctgagt gatctgcagt gcaatccctt tgatatgatc
     5461 gggaagacat ggtacttact gacttccggt acaccggagg attacaacac aatgacggct
     5521 tcctgggggc aatgggcgaa atgtggggag caccctcgtt ccactgcgtc gtgagaacca
     5581 accgttatac cctgaactat ctggatcaga atgagctgtt ctcggcaaca ttcttcgatc
     5641 cgggctataa acccgcactt cagttctgcg gcactcattc cggaaaggac tgcgataagg
     5701 cacagagaac cggattgcag cccgttctcc ttgaaggaac taccagcttt gcacaggcac
     5761 gccgcgtgct gatctgccgg aaacgttata cggcaatgtt gcagccggag ggttttctgg
     5821 aacccgaaac ttatgaaaaa tggtacggca aagacccgat gcaccgggaa tttgtaggcg
     5881 aagtactggc gtattacgaa agaatgcact gatcgttggt gcacgcaaag agcctctgtc
     5941 atctgacagg ggctcttttg gtatctgtac gccgctgccc aaagtgcatt tttttcacga
     6001 aagcacaata atatgcaaaa ataactgcat attatacgtg aactgttgca aaaagaaaaa
     6061 aattccgtat atttatgcaa aaacgcttga caatgccgga aaaaggtgta taataagaaa
     6121 caagagcatt tgcagcggat caaaaagggc ggtacagatg cgataaatta tgctgatgga
     6181 ggagtttgca tcatggcaaa ggcaaaaaag gacattaaga aagtcgttct ggcatattcc
     6241 ggcggtctgg atacttcgat tatcattccg tggctgaaag aaaactataa caactgcgaa
     6301 gttatcgcag tttccgcaga cgtaggacag ggcacagaac tggacggtct ggaagaaaag
     6361 gcagtcaaga ccggtgcttc caagctgatc gtactggatc tgaagaagac tttcgtagaa
     6421 gactacattt tccccaccgt acaggctggt gcggtatatg aaaaccgtta tctgctgggt
     6481 acctctttcg ctcgtccgat catcggtaag gaactggctc ggatcgcact ggaagaaggg
     6541 gcagatgcga tctgccacgg tgcaaccggt aagggtaacg atcaggtccg tttcgaactg
     6601 gcaatcaagg catacgctcc ggatatggcg atcattgctc cgtggagaga atggaccatc
     6661 aagagccgtg acgaagaaat cgactatgca gaggctcaca acattccgct gaagatcaac
     6721 cgtgaaacca actactccaa ggataagaac ctgtggcatc tgtcccacga aggtctggat
     6781 ctggaagatc cggcaaacga gccgcagtat gaaaaggaag gcttcctgga actgggcgtt
     6841 tctccgatcc aggctccgga caagcccact tatgtgacca ttcactttga aaagggtatc
     6901 ccgactgcta tcgacggcaa ggaaatgggt gctgtagaac tggtagaaaa gctgaacgaa
     6961 ctgggcggtg caaacggcat cggtctggct gatctggtag aaaaccgtct ggtcggcatg
     7021 aagtccagag gcgtttatga aactccgggc ggcaccattc tgtaccacgc acacgatgtg
     7081 ctggaaacca ttaccctgga caaggaaact gcaagaacca agcagtatct gagcatcaag
     7141 ttcgctgaca tcgtatacaa cggtcagtgg tacacaccgc tgcgggaagc actgactgct
     7201 ttcgtaaacg aaacccagaa gaccgttacc ggtgatgtca agctgaagct gtacaagggc
     7261 aacatcatca atgccggcgt tacttctccg tacaccctgt acgatgaaga agttgcaacc
     7321 ttcgatgcag aagacgttta caaccagaag gatgcagagg gcttcatcaa cctgttcggt
     7381 ctgccgatcc acgtaaaggc tgtgctggat cagaagcgga acaacaagta attttgaaaa
     7441 aatcgggaaa cacggcggtg atttccctgt ataccggttc tcctgccggt gaggaagagg
     7501 cggctgttta ctggtcgcct cttttccgtt tcaaaggaat tacttcctaa tttttaaaca
     7561 atagaaggga ttcataatgg ctactattca gcttgacttt actaatctat gtgcggcaca
     7621 ggatatgatt gcaattattc aaaaagagcg aaattgtaca gttcaagaag caattttgtt
     7681 ttcaatcaat tctgcaaatt gtaagagtat catggccacc ggttgggctt cgattgcata
     7741 ttcactttgg ggtcatgacg atccggaaag agagtggctt tttttgaact ctcctaaggt
     7801 ggacgttgaa tttagcgaaa cggatattaa atatcttaaa aaagtagcta aagcagaaaa
     7861 agtgaaagtt gtaacagcag tatgctattt tcttgtattt acaatgcaat cattaggcta
     7921 tcatatataa tgatttgagt aaaaatcacg aaaggacgtt tcatatatgg cactttgggc
     7981 aggaagattt tccaaagaaa ttgacgcagg cgtaaatgcg tttaactcct ctatcgcatt
     8041 tgatgcccgt atgtaccggc atgacattca gggcagtatc gcccatgcca ccatgctggg
     8101 ggactgcggc atcatctcca aggaggacag caagaccatt attgaggggc tgactgccat
     8161 tctggcagat ctggacagcg gcaagctgga atttgatccc aatgcggagg acattcacat
     8221 gttcgtggag gcagagctga ccaagcgtta cggcgatgtg ggcaagcggc tgcacacctc
     8281 ccgttccaga aacgatcagg tggcactgga tctgcggctg tatctgcggg acgagatcca
     8341 ggagatccgc ggactggtgg aaaagctgtg tgccgtgctg gtgaagctgg cagaacagca
     8401 caccgaaacc gtgctccccg gctataccca tttgcagcgt gcccagccgg tgacttttgc
     8461 acaccacctg ctggcatatg tacagatgct gctccgtgat ctgggcagac tggacgacac
     8521 agcaaagcgt atgaacgaat gcccgctggg cagcggtgca ctggctggca ccacatacca
     8581 catcgatcgg gaagaaacgg cgaagctgct gggctttgat gctcctatgg caaacagtct
     8641 ggacggcgtt tccgaccgcg actactgcat tgagctggcg aataccctct cgatcatcat
     8701 gatgcatctg tcccgcttct ccgaggagat cattctctgg tgctcgtggg aattcaagtt
     8761 cattgaactg gacgatgcat tcgcaaccgg ttcgtcgatc atgccccaga agaagaatcc
     8821 ggatattacc gaactgatcc gcggcaagac cgccagagtg tacggcgatc tccagaccct
     8881 gctgaccatg atgaaaggtc tgccgctggc gtacaacaag gacatgcagg aggacaagga
     8941 agcgattttc gacgcagtgg acaacgtgaa gctgtgtctg gaaaccttta cccccatgct
     9001 ggaaaccatg cgggtgctga aagacaacat gcgtgccgcc gcagccaagg gctttatcaa
     9061 cgccaccgac tgtgcggact atctcgtgaa gaagggcatg ccgttccgcg atgcctataa
     9121 gatttcaggc acgctggtgg cagagtgcat ttccaggggg ctgacactgg aaaccctgcc
     9181 gctggaggaa taccagaaga tgacaccgct gttcaccgag gacgtgtacg atgccattag
     9241 tctggaaacc tgtgtccgcg gcagagtgtc gcagggcggt ccgtccccga aggctgtggc
     9301 agaacagctg cggctggtca agacaaagct ggggctggca tgagaaagcg gcagatcaag
     9361 tttattctgg cgttgtgcac tgccgcggta gtgctctggg tcggcggaga gatcctgctg
     9421 ggactggtgc tggatctggg actgacagtg tcccgcatct gggcaatcgc cattatcgtg
     9481 ctgacctgtg cgttcagtct gtggaaatgt ccgcgggaat aggagaaatc atgagaaaat
     9541 ggatcgggat cctgatggcg gttgggttct gcctttgcac tggctgcggc aggacggctt
     9601 cgcctgaagc ttcggaaacg gagacatcgt ccgtagtgac aacggcaaag gtacaactag
     9661 atgcggtgca gacaactgct gctgtgcagg aggacgatgc cgcggcagtg acgaccaaga
     9721 agaagactgt ggatgtgccg aacgggaaaa agctgacggc ggtgactcca aagcaaacgc
     9781 tggatactgt ggaaaagcag gagatcacaa ctgcggaaac gcagagtacg actgccatag
     9841 aaatggtata ctatagtgca actggctctt attggcatct atcggaggaa tgtgccaaag
     9901 aggattatgt ccacgttgtg tacaatatgc agggagagac aatgcctgca ccgaagccgc
     9961 acataataaa atcgccgaaa acaatgatcg tcggcggaaa aacgccctgc ccgaattgtg
    10021 cggcagggga atgaggtgga aaaatggctg taaagatatt tattgacggg caggctggca
    10081 ctaccggtct gcggattcat gaacggctgg cagaccggaa cgatctgacc atgattaccc
    10141 tgactgagga aacccgaaag gatccggagg caagacgggc tgccattctg gaaagcgacg
    10201 tgacattcct gtgcctgccg gacgatgcgg cgagagaagc tgtggcactg gctggcgatg
    10261 cggacgtgcg gatcattgat gcttccacgg cacaccgcac caatccggac tgggcatacg
    10321 gctttccgga actgtcgccg gagcatcggg aaaagattcg ccactccaag cgtgtggcag
    10381 tgccgggctg ttatgccagc ggcttcgctt cgctggtgta tcccatggta cacagcggac
    10441 tgctgcccaa ggactatccg gtgcagtgct tcgccatgtc cggctacagc ggcggcggca
    10501 agaaaatgat cgccgagatc gaatctcccg accgtgctcc ggaactgtac agcccccgtt
    10561 tttacggtct ggcaaacaat cacaagcacc tgccggaaat gcagaagatc tccggtctgg
    10621 aaaagaagcc catgttcacc ccgattgtag atgattacta taacggcatg gtggtctgcg
    10681 tgccgattca ggcggcaatc tccggcttct ccctggaacc ggaacgggtg caggaaatgc
    10741 tggaacgcca ctatgccggt tccaactttg tgcacgtgca gcccctgaaa acaccggcag
    10801 aaatgacagc caccaagctg agctgcaacg atctgcggga taccaaccag atggaaattt
    10861 tcgtgttcgg tgatgcagag cagatcttgc tgtgctctcg tctggacaat ctgggcaagg
    10921 gtgcctccgg tgcggcagtg cagtgcatga atatcatgct gggactggac gaaactgtag
    10981 gacttttgta agggaaacaa acgctgcgga ggaataacca aatgaaacaa attgaaaaag
    11041 tgctgtatga cgggtttacc tatatcgacg gcggcgtttg tgccgctaag ggatttcgtg
    11101 caaatggcgt gtactgtggt atcaaggaaa accccacgaa aaaaccggat ctctgtctgg
    11161 tcgtatccga caagctgtgc cgtgctgccg gcgtgtttac ttccaataag gtgaaagctg
    11221 caccggtgct ggtttccaag gcacatctgg aacagaccgg cggtaaggca tggggcatgg
    11281 tgctaagctc caagaatgcc aactgctgta atgcagacgg tattgacaag gcgaaccgtg
    11341 tctgcgaact ggcggcggaa caaatgggcg ttcagccgga agaactgctg atcgcacaga
    11401 ctggtgtcat cggtcaggtc ctgccgattg agccgtttga acagggcatg ggcaaggtgt
    11461 atgacggctt gtctgctgaa aatcatgagc aggcagccat tgccattatg acaacagata
    11521 ccgtgccgaa agaagtggca gtggaattcc agctgggagg aaagacctgc cacgtaggtg
    11581 caatgggcaa gggcagcggt atgatctgcc cgaatatggc aacgacgctg aatgtggtaa
    11641 cgacggactg tgcgatctct gcggaactgt tacagacagc cctcagtgat gtggtacagt
    11701 atacgtataa ctgcctgtat atcgacggcg accagtccac caatgatacc tgcgtggtac
    11761 tggcaaacgg tctggcagaa aatgcggaga tcactgaaca gaatgacgat tatgccgtat
    11821 tccgtcaggc tctgtatatc gcgatgtcca acatcaccaa gatgctggca aaggacggcg
    11881 agggtgcgac aaaactgctg gagtgtactg tgaccggtgc gaaagatcgg gaaaatgcac
    11941 tggcagttgc caagagcgtg atcgcttctg atctgctgaa atgtgccatg tttggtgcag
    12001 atgccaactg gggacgggtg ctgtgtgccg tgggctatgc accggcagaa ttcgacatcg
    12061 atcaggtgtc tgtgacactg gcttcccgtg ccggttctgt ggacgtgtgc aaaaacggtg
    12121 ccggcatcga cttctccgag gacatcgcca agacggtgct gctggaggac gagatcgaga
    12181 ttctggtgtc tgtcggtgac ggtccggcgg cggcaacggc gtggggctgc gacctgacgt
    12241 atgactatgt aaaaatcaac ggtgactacc gttcctgaga gaggcttcga gaaatgggag
    12301 agatattgaa caatgtgcgg gcacaggtgc tggtagatgc cctgccctat attcagaaat
    12361 acaacaacaa gattctggtg gtcaagtacg gcggcaacgc catgaccaac aaggaactga
    12421 aacaggctgt gatggacgac atcgtgctgc tgtccttggt aggtgtcaag gtcgtgctgg
    12481 tgcacggcgg cggtccggag atcagcgaca tgctgaagcg gctgaacatc gagtccaagt
    12541 ttatcggcgg tctgcggtat accgatgagg cagcggtgga cgtggtaaaa atggtgctat
    12601 ccggaaaggt caacaaggaa ctggtcagcc tgctgacacg gacacggggc aaggctctgg
    12661 gaatgtgcgg catcgacggg cacatgctga cggcagaacg gaagctgggc cccaacggcg
    12721 aagatctggg ctttgtaggc gatattgtca aggtcaacga acagcccatt ctggacgctc
    12781 tgaacaacgg ctatattccg gtcattgcca cggtggcaac cgatgagttc gggcagtcct
    12841 ataacgtcaa cgcggataca gctgcggcaa gaatcgcggc acagctgcgg gcggaaaatc
    12901 tgattctgat gacggacatt gcgggactgc tgcgggacaa ggacgatccg tctaccctga
    12961 ttccgtttgt caacgtcagc gaagtgccgt tcctgaagcg gaagggcatc atctccggcg
    13021 gcatgatccc gaagatcgac tgctgcgtag aggcagtccg ccgcggtgtc aagaaaacgg
    13081 cgatcattga cggcagagtg ccccactcca ttctgatcga aactctgtcc aatgagggca
    13141 tcgggacaca gttccgataa gcacagaaag gatttgaaag aatgggtttg ttagaacgtg
    13201 ctgcgatgga agcagcaaag aaaatggcgg aaagtgaaga aagccacaca gatgtcaggg
    13261 gcattgtaaa agcttctaag gtgtttccgg tggtttctat tgcctctatt ctgctggctt
    13321 tgggaatggc atttctgaag cacagacaga gtgatgcggc gttttatgta ctggctgggg
    13381 tgtttgcagt gctgggaatc tgcctgctga tctatgggca caatttcaga atcgagtacg
    13441 accgcacggg aattacggtg tacaactttt tcggaaagcc caagcagtat accctgcagg
    13501 acgtgcagaa gatcgaactg aaaagcgatg gctatattgt gtggactgcc aagggaaaaa
    13561 ttcgggtgga acagcagttt ttcattggtg cagatgcatt tctctgctat ctgcgggact
    13621 atcagaagga ggaacaggta tgaagttgac atctgcaaga aaagattatg ggatttacgg
    13681 ctttatggta gtggtgtttc tggcggggac aattgcattt tttgtgcggt acggtacaag
    13741 tgagatctgg tacacgctgc tgatggtagc attcctgctg gtcagtctgt attttttcac
    13801 ctgtacgctg cggtttcggg tgatgtggga cgatgacggc attcatatcc ggcagaactg
    13861 gaaaccggaa gtgcactaca cttgggacga ttttgaacag atgtacataa aggaccagtg
    13921 catcatgctg cacctgaacg gcaggcggta cggcttttcc caaggctgtc ccaacgcaat
    13981 ggaactgatc gaatttttgg aacagaaatt ttccgctgaa caggcggcag aataaaggaa
    14041 atgataacta tgacaacaca agagcaaatg aaccagtccg tcatgggaac atacggacgg
    14101 tttccggtgg tgatcgatca cggtgcaggg gagtcctgtg ctgacgaaac cggaaagcag
    14161 tacatcgact tcggcagcgg catcggcacc acctctctgg gttactgcaa tgaggcgtgg
    14221 acggaggcag tctgcaagca ggtcaagacc atgcagcaca cctccaacct gtactacacc
    14281 aaggtgcagg cagacttcgc acagaagctc tgccagactg ccggctatga aaaggtgttt
    14341 ttctgcaaca gcggtgcaga ggcaaacgag tgtgccatta agctggcgag aaagtacagc
    14401 ttcgacaagt acggcaaggg cagacacaag attctgacgc tggtgaattc gttccacggc
    14461 agaaccctct gcaccctgtc cgccaccggt caggacgtat tccacaacta tttcttcccg
    14521 tttgtggagg gcttcgacta tgtgacagcc aatgacattg ccgatctgga atccaagctg
    14581 gacgacaccg tttgtgcagt gatgctggaa tacattcagg gcgagggcgg cgtgaatgca
    14641 ctggagcagg cattcgtgga cgctgttttt gaaaagtgtg ccgcaaagga cgtgctggtc
    14701 attgcggacg aagtgcagac cggtgtgggc agaaccggta cattccttgc cggtgaacag
    14761 ttcggacaca aggcagacgt gacgacactg gcaaagggca ttgccggcgg tctgccgatg
    14821 ggggcatgcc ttgccaacga gaagtgtgcc ggcgtgctgg acaagggctc tcacggctct
    14881 acattcggcg gcaatccggt atgctgtgcc ggcggtctgg cagtgctgaa caccattacc
    14941 gcagacggct ttctggcaga agtgcagcgg aaagccaagc tgctgcggga gaagctggaa
    15001 aagctggacg gcgtggagag cgtcagcggt ctgggactga tgatcggcat tgccctcaag
    15061 gacaagaaag ccatcgatgt ggcaaatgcg gctctggaaa agggcctgct ggtgctgacg
    15121 gcaaagacca aggtacgtct actgccgccg ctgaccatta cagacgagga aatcgaaaag
    15181 ggcgttgcca ttctggcgga cgttctgtcg aaataaagga aacactgcgt gaagcccgcc
    15241 tgacggcttt gcacgcaatc tgcttgcaga ttttcagctt tatgcagtgt ttcctaaacg
    15301 gaggagttag actatgaaac atttactgaa aatgctcgac ctgagcaccg aagagatcat
    15361 tgagattctg gatcttgccg atcagctgaa gtacgagctg aagcacggca ttccccaccc
    15421 gcatctgaaa ggcaagtcgc tgggcatgat cttccagaag gcatctaccc gtacccgtgt
    15481 ttcttttgaa accggcatgt accagctggg cggacacccg ctgttcctgt ccgcaaacga
    15541 tctgcagatc ggcagaggcg aaccggtgca ggatactgcc cgcgttctgt cccgctatct
    15601 ggacggcatc atgatccgta cctttgagca gaaagaagtg gaagatctgg cagagtacgg
    15661 ctcgatcccg atcattaacg gtctgacaga cttctgccac ccgtgccaga tcctcgcaga
    15721 cctgatgacc attcgggagt tcaagagcag cttcgacggt ctgaagatgt gctttatcgg
    15781 cgacggcaac aacatggcaa actctctgat cgtgggctgc ctgaaagtcg gcatgtccgt
    15841 ttccattgcc tgcccgaacg attaccagcc ggatcaggag gtactggact ttgcggcaca
    15901 gtacggcgac aagttccaga tgactgacaa gccgctggag gctgccaagg acgctgacgt
    15961 ggtaattacc gacgtatggg cttctatggg gcaggaaggc gaagcagaga agcggaaaaa
    16021 ggcattccac ggctatcaga tcaacgatga agtcatggca caggctcacg acgacgcaat
    16081 ggtactgcac tgcctgccgg cacaccgcga agaagaaatc accacaaagg tattcgaggc
    16141 acatgccaac gagatctttg aggaagcaga gaaccgcctg cacgcacaga aagcggttat
    16201 ggtaaagctg atggcgtaag gacgctgtcg ttctataaac atcatgaagg gcattctgta
    16261 aaaaacagag tgcccttttt atgatatgct tgacaacaga aaatgaaaaa tgtataataa
    16321 aaagaaatat tatatgggaa aatccctgcg acaggagcga aatgagcatg tataaaaaaa
    16381 ttttagtcgc aattggagtg atctttttga ttttctttat tgtgaagctt tgcgaaaagg
    16441 gaccgtcaga gccgagcagt tttgattcga tttatgaaga aagtcaagaa gaatcccaag
    16501 aagaaaccag tacatacgac aactccgaca gcgtggtcac acgtaagcgg gagtattctt
    16561 ttccggagta taacagcgat accgatgaaa ttgacgatga atacgagggc gaagattatg
    16621 atgactacga tacaaactgg tttgatgatt acgacgacga tgagggtgat tacgactatt
    16681 agcgaaataa acaaaattca acaagaatat tttacatcaa ttttacttga caaagtcaaa
    16741 acagcgtgat ataataataa agctgtcgca cgagagcgag agttgaggaa acttgtgaat
    16801 aacggtaact gtgaaacgga tcgggagatc gacagttagc ggttggagtt gttcaagaaa
    16861 a
//
LOCUS       DAWBGR010000037        16604 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1163_length_16604_cov_3.423893, whole genome shotgun sequence.
ACCESSION   DAWBGR010000037 DAWBGR010000000
VERSION     DAWBGR010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16604)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16604)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16604
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1163_length_16604_cov_3.423893"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    15309..15318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3864 a         4746 c         3903 g         4081 t
ORIGIN      
        1 gacgaaaaat ttgacgacgc atctgtcgca caatctttgt gcggtattgc cttgattctc
       61 actgaaaagt tacttccacc ttgccgtcct tggcggaaac cagcagtgtg tgaatttctg
      121 tactgtgtgc aataatcaga gaagcgatct gatcttctac gatctggcga ataacacggc
      181 gaatgtcacg ggcaccgtag ggcttgccgt atgcttcttc tgcaatggct gccagtgcgg
      241 cagcatcgta ctgcaacgtg atgttctttt ctgccagcgg ctgtttcatt tcgtccagca
      301 tcaaagcagc gatcttttcg aaatccggct ttgtcagatt ccggaacacc acgatttcgt
      361 caatacggct gatgaactcc ggacgcagga attcccgcag tcccttcatt gccttttcct
      421 tggaaatttc agtgtctgtc cggttaaagc ctacgccaat gctcttatcg gtagagccgg
      481 cattggaagt catgcagatg acagtgttct caaagttgac cgttctgccc tgtgcatcgt
      541 cgatcttgcc ctcgtccaga atctgcataa gaatattcat cacgtccgga tgagcctttt
      601 cgatttcgtc gaacagaatc accgagtacg ggcggcggcg aactttttca gtcagctgac
      661 cggcttcatc gtagccaaca tatcccggag gggaaccgat catacgtgcc acagagtgct
      721 tttccatgaa ctccgtcatg tccagacgaa tcagcgggtc attggaatcg aacagttcct
      781 gcgacagcac cttgaccagt tctgtctttc ccacgccggt aggtcctaca aagataaagg
      841 aagccggacg gcggcgaggg cttagttgta ctctcgtccg cttgattgcc tttgccagcg
      901 aatccactgc ttcgtcctgt ccgatgatat gggctttcag acggtcttcc agatgtgcca
      961 gcttggtgaa ttcagtttcc gcgatcttgt gtgccggaat ccctgtccac agttcgatga
     1021 ccttggacaa atcgtcagca gtcacctgca cttcgtccag cttcttctgg gcttcttcca
     1081 gactggtatt cagacgaatc agttcgccct tgacctgagc cagttcttcg aagttgatct
     1141 cggtttcatc ttcaatggat ttttccttgg cttccagttc ttcctttttc tttgccagct
     1201 tatcataagc ttcgatttcc ggagaacgca gattggtgca tgcacaactt tcgtccagca
     1261 ggtcaattgc cttatccggc aggaaccggt cggtaatata ccgttctgac agcacagcac
     1321 acagacgaat catctccgga gaaatgtgca cacggtgata ggattcgtag tactttgcaa
     1381 tgccgttcag cacctcaatg gtttcgtcta cagtcggctc attgaccgtt accggctgga
     1441 aacggcgttc cagtgcagaa tccttttcga tatatttgcg gtattcttca aaggtggtgg
     1501 caccaataac ctgtacttcc ccacgggaga gtgcaggctt caggatattt gctgcattca
     1561 tggcaccctc agaatttccg gcacctacca gattgtgcac ctcatcgata aacagaatga
     1621 tgttgccttc ctgcttcacc tctgccagca agcccttgac acggctttca aactgtccgc
     1681 ggaactgggt accggcaacc agtgaagtca gatccagcag gtatacctgc ttatctttca
     1741 gatgcatggg aacgtttccg gagtggatcc gcaaagcaat tccctcggca attgccgttt
     1801 tgccgacacc gggttcaccg atcagacagg gattgttctt ggtgcggcgg gagagaatct
     1861 gaatcacccg tgcaatttcc ttatcccgtc caatgattgc gtccagcttg ccctctgcgg
     1921 cacggcgagt caggttcgtg cagtaattgc ccaagaaccg cagttccttt ttccgcttgg
     1981 aacggcgttc ccgtctggaa ccgcttggga tttctgtgta gtctgctgac ggaatctcct
     2041 catcaccgtc tgcggtctgc aattcattgg attcctcacc gtcatctttc agattcccga
     2101 aaatctttcc cggatccttc atgaaagatt gcaggaactg cggcattaca ccggagccgc
     2161 ccatttcaaa gctttccccg tccatcatgt ccatcatctg attggagatc tgctccagtt
     2221 cctcatcggt aattcccatt tgtttcatat attcatcgat ctgcggcacg tgcagttcct
     2281 tggcacacac cagacacaga ccatcatttt tcttcttttc gccctgcatc gctgtaataa
     2341 ataccacggc aggccgtttt ttgcatcgac tgcataacat catcgttttc tacacacctt
     2401 tctgataggt ttattgcatc agctgataga gatgctgaaa taaaacagac ttctgaattt
     2461 cagcctgatt gaaaaaggca atctgatttt cgccgtgctc cacgatccac cccgtcagtt
     2521 tgaccatcag caccagcaga aaaattgcct gcactgctcc cagtccggca ccaactacct
     2581 gatctgtcag gcgtatgccc tcggtgcggc ggaaagcaga tagtcctttt attatacctt
     2641 gaatcaatac agaaatcaag agaaatagaa ctgaaaaagt aaatgtttta accattataa
     2701 tcagcaccgg acgaacatac agctttgtca gctgctttgc agcatcgctg gtattgctgg
     2761 agagcaccat tgccgccgcc tgtacagtcc ggttttgcag ttcctgtgaa ctctgctgcg
     2821 gcaggatcgc ctcgtgcatc catgacggca tctccgtaaa cagctgagaa gatgcactgt
     2881 caatactctg ggacagtcct tgctgcacta gttccgcagg cagtcctgtt ttctgggaca
     2941 ccacgctgta caactcttcc gaagcattgt ccggattgtc tgcgatctgc tgtaatgccg
     3001 catcgtccag ctgaatccca taatttccca gcgtctgctg taaatcgtct gccagatgat
     3061 actgttccat tttctgttcc agaatcgccg cacatggctc tgccagccag cggtcgtata
     3121 catattctgc cgccggctga gccagaacgc ctgccagcaa tgccgcagca gcatatccca
     3181 gcagaccagc aacagaactg atcagacccc gccgccagcc gccccagata cagaggatca
     3241 gcactgccaa cactgtcaga tcatacagcc accacgtctg atgcatgaaa gattccacaa
     3301 gctactcctt aaagtccaca cgatcaatct gatcatatcc cagcaggaac aaataaccgt
     3361 cctgtttcag aaacgaagta taggcaccgt ctagatttac tgttgccagt gccgtaccgg
     3421 aaaaatcata ggaagtgata tttccgcggc tcatgacaat agcatcgccg tcgcacaccc
     3481 gtacttcttc cgcagtgctg tcaatagaca acactttcgg actggaaaca gactgatcca
     3541 gaagcaccag cgaggtgttt cgcttctggt cgtcctgtat gatcagagca gcctttccgt
     3601 tttccagatc tgcactgacc agtgtgccgt tatagttata tgttccctgc atttcgccgg
     3661 cactgctata gtatgcacaa gcagtatttc ccaccacaca gaatttgtcg ttggaggaaa
     3721 aaccagattt gacgctcaaa gtttccagcg gcatggaagt ccactgggtg tccacctgat
     3781 taaatgtgat gctggtgaca gtgctctgca ccacaccatt ttccactttc actgaagtca
     3841 gacacaagcc gttgctgtcc ttgtgaaatg ccagatccac cacgtactcc gtacagttcc
     3901 gctgatagag ccgctttccg gtattgtcgt ataccacaat ggcacaggca taggagccag
     3961 actccgtaat cagggcaaca gttccgttgt cgctgacgac accggaaaga atgttgttgt
     4021 ccacagactt gccatagaca tttttgtgcc tggtgtccag ccggaatgag gtgccgccgt
     4081 tttcgaacag caatacatat tttccggcag tattcatggt ggcgttctgg aacgcgtgct
     4141 gccggttatc tcctgcatcg ccgtgcatag aataggtact cagataggca tcatgcagca
     4201 gaaacaaggt gtcatctgcc aacgctgcct gatagctgga acttccggaa atggtcagcg
     4261 ggaagtttcc ctctgccagt gtgccgtcat tttgcgtcac agaatcgtac cttccgccga
     4321 tcccctccag cttcgggatc cacgcgtccc gctgtatata caaatacccg cagaagccga
     4381 cgatcagcag gaatatcagc aaattccgca gagaacgcag cttactgcgt cgcttgcgtt
     4441 tttcgatcat atcatcagaa tcatagctca taagcttttt gacgatctcc tttccgacag
     4501 gctttattca taaaacaccc gattcaggta cagtccatgt gcctgtgcgg tgattccggc
     4561 gaggacacga tttttagatg ccagaaggtc gtccagttcg gaaacggcga ttcttccctc
     4621 gttgacactc aacagcgtcc ccaccagtat cctaaccata ttatacaaaa aaccattgcc
     4681 tttgacaagc agaatcacgg tatttccttg cttttctatg gttatgtcgt ggattgtacg
     4741 aatggtattc tgtttttctg tgtaatttgt acaaaacgtt gcaaagtcgt gggtgcccac
     4801 aaagtgctgt gccgcctcct gcatcagcgg aatatccatt ttccgccgat agagcaattc
     4861 cagatcggtg gtaaacggat tcttgctttc gctgttgtgg atccggtaca tatattcctt
     4921 tgccttgcag ctgaaccgtg catgaaactc cggcggcatt tcctccaccc gataaatgga
     4981 aatatcctcc ggcagatacc cgcaggctcc ccgaatgaaa ttccgttccg gcagctgttt
     5041 ttccgtctgc atggtgaagc agtactcctt ggcatgcacg ccggtgtctg ttctggagca
     5101 gccctgtatg gtcaccggct cgttgaacag ctgcgacagt gctgtttcca ccacttcctg
     5161 cacagtgatg ccgttgggct gccgctgaaa cccgtggtaa ttggttccgc gaaagcccat
     5221 tgttactttc agatttctca cgattctgtt tcctccgcct tttctttttc cggaacagca
     5281 aattctgcct gttcctccgg aaccggctgc aacagccgct ttccggtttt ccactgcaca
     5341 aaagacacgg caagcagcag taacaccgca ccggcagtac agcaagcccc tgccacaaaa
     5401 cctttcgggc aataggtcag cgtaatggta tgcgttcccg cagtcaccgg aacggcaacc
     5461 atttctccgg caatgggtgt gatctcggtt tttacgccgt ccactttcgc ataccagccg
     5521 gattcatagg gaatcgaaaa atagcatacg ccgtcctgct ccgcagtcag agtgccgcta
     5581 agcttagtgt ccgaagcttc cgtcagcgta atgccgccgt tctgcaatgc tgcaaagccc
     5641 tgctccagca cgtccggatt gatctgatac atatacagca cgatcagacc ggaaactgcg
     5701 tccttgggca aggtcataga aacacttgcc gtatccgctc cctgataggt gcccattggg
     5761 aaaatgaatc cctgcttgcc attgttgaaa tagcccttgt agacgccgtt ttgcagaatc
     5821 gtaatattgg aaacgtcctg caaattcatg taaccataca gtgttgtatc cagcggcacc
     5881 cgataattgt attgcaggct gtgagattcc gcactggcat cggtatgata cgtgtagttg
     5941 ccatagctgt ttttcagggc atctgctccc tcataatcca cgctgtccac ttccagcatg
     6001 gtaaacaacg gtgtttcaat gccggtcgcc agccggaaca gcgtgttcag attatcgaac
     6061 gggttcgcct ccggattcgg atcgtattcc agcaaagagg cattggcacg gaagcctatg
     6121 ggaagatcgt actgattccg gtacgcatag caggactccg aagaagcgat ctgctcccac
     6181 gcactgtcat ccagcactgt gcttgctcgg ctgattagat agcgaacacc ggtaaaggca
     6241 tttcctaccg gtgtgccgcc gccgtagtag taacggtttc ccgcctctga tgccggaaag
     6301 cccagcgaac ggatccactt ggtggcattt tcatttgcca tggaagaaaa ctgcgaaacg
     6361 ccgcgataac catacaacgc cggatcattc agcgtatacc atgtggaaag ttccgtccgg
     6421 taaaacggcg tttcgtcctg ttcatcgatt tgttccagca gattttctac gtctgtatag
     6481 gaagccggat agccggtata tgccgatgtt gaaaccgttt ctgtaccgag cttcacgttc
     6541 tggaacatct cgaatgccag cacgatcgac accgccagat acagcatctt ctttccgaaa
     6601 atctgccgat atgccaatag cataatgcct gtatacagca gcatcagtgc ggctgtccag
     6661 tagacagcac ggttttcctg cacattgtat gcaatcagga acacacctac tgtcactgcc
     6721 agcattgcca gcaagtccca cacgcggcat ttttcctcca gcacgaccaa gaaagcacga
     6781 tagcccactg tcagcagggc aaaggaaaac aggaatgaga atcgataagg cagcatattc
     6841 ggaaaatgga atccgtgcca gatatagttc agcacattgc agttacagct gatcaccaag
     6901 aacgccagca ccagaactgc catgatcttt tcccgaatcc gaatcctgcc ggaccgcaga
     6961 aacggtccga tcaacaccag cggcagtaca ccgcacgcca gattcggcag accgtccttg
     7021 gaagtcggtt catgaaagct gatgagattt gccgccagcg tccgccagtt ttcataaaac
     7081 tgcactgccg tagggaacgc attgttcaca ctataggtca gctgcaatgc gtaataagtg
     7141 ggtatcaaca caattgccgc caacgctccg cccagcaaag aacaacctgc cattgccaat
     7201 gtccgcatcg gcagctgttt ccagcgaaga tagcacacac acagacaaca gtaggcgatc
     7261 actgcaaaga tgcaggtgaa catgccgata tagaagtttg tgaacagcga cagccccagt
     7321 gcaatgcagt acagccggca ctttccgtcc cgcaccagat ataccagtcc cagcactacc
     7381 agcggcagca gggcgactgt atccagccag atgatattcc agtagtaccc cagaatgtag
     7441 ctgcacagag catacatcac ggaaaagata cagagggaaa aatcatttct ccggaatgtg
     7501 ccgcggagaa agcatgcgaa aaacaagccg gcaaagccga ctttcaacag caataccaat
     7561 gttaccgcgt cacgcagaaa tgtttccggc accagtgccg tcagcaggtt cagcggactg
     7621 gcagcatagt aggcaaaaat agatacgaag ttggaaccca gaccggaatc ccatgtatac
     7681 agcagagaac ctccatgctg caatttttca tgcagcagcc gcaggaacgg ataatactga
     7741 tgccagaaat ccaccaccag aatctgctga tctccaaaag ggtgcacgtt ctgcagcaca
     7801 agccctgccg ccagaaacag catggggata ccaaacgaca gcagcagaaa aatgcggctc
     7861 ttttgacttt ttacatttgt ttgagaaagc ggcgttttca cagacggtgt tatttccatg
     7921 caaattctcc ttaaaagatg atttttaccg ctaccgcacc ggcaaacaac agcagcgtca
     7981 ctgccattgc tgcataatcc tgtccggcag catgcagctg ccgcattttg gttctgccgg
     8041 tttccccgcg gtaacagcgg cattccattg ccgttgccag ttcttccgca cggtgaaaag
     8101 acgacaccag cagcggtacc agaatcggca ccagtgcctt ggcacgctgc aacagattgc
     8161 cctcactgaa gctggctccc cgtgctttct gtgccgtcat gatcttgtcc gtttcttcca
     8221 gcagcgtggg aataaaccgg agagaaatcg tcatcatcat ggcaaatgta tgcaccggca
     8281 catgcagctt tttcagcggg gacagcagcc gttcgatggc atctgtcaga gcaatgggcg
     8341 aagtggtata agtcagcagc gaagagcctg ccagcagcac aatgattcgt gtaaccatta
     8401 aaaccgccgt ccgcacgccg cctgctgtga ttttcagaaa atgccagtga agatacacat
     8461 caccgctgtc caccatgaaa aggtctaaaa ttgctgtgat cagcagcacc ggtatcagcg
     8521 gtttgatgct tttccagatc agcttcaccg gaatgcggga gagagccagt gccagaatgc
     8581 aaaacacaat tccgaaacaa atggagtaca gatgctgtcc cataaacaac atggcaataa
     8641 acaacagcgt catgatcagc ttgaatcgcg gatccatgtg gtgtaccgga ctggagccgg
     8701 ggaaatattg tccgatcgta atgtctttca gcatgttccc tgccctcctc ttgtccgatg
     8761 ataccattcc agaagctgct gttttgcctg ctccacggtg aaaatatcat ctgtcagcgg
     8821 tacgcccctg gcacggagtg catcacaaac gcgggtgatc tgcggcactg ccagaccgat
     8881 cgcctgcaat tcctgcgaac ggcggaaaac atttgccacc gtatcgtaac agaacacctt
     8941 tcccgcattc atcaccagaa tttttttcgc atgccgtgcc acatcttcca tgctgtgaga
     9001 aaccagcaaa atagtcgctc cggtctgatt gcggtacgcc tggatctcct gtaggatctc
     9061 ctcccgcccg cggggatcca gtccggcggt aggctcgtcc agaatcagca cctccggacg
     9121 cattgccata acgccggcaa ttgccacacg gcgtttctga ccgccggaca gcaaaaaagg
     9181 tgactgcttc aggatttcct cggaaagccc cacaatggca gccgtttccc gcacacgtgc
     9241 ctgcacttct tcttcggcaa gtcccatatt ccgcggacca aaggcgatgt ctttcgccac
     9301 ggtttcctcg aagatctgat actccggata ctggaacacc agtcctaccc gaaaccgcat
     9361 ctgccgcatt ttcgacttgt ctgcccagat gtccacaccg tccagcagaa cctttccctc
     9421 tgtgggacgc agcagcccgt tcaggtgctg aatcagcgtg gatttgccgg agccggtatg
     9481 cccgataatc cccacaaagg agcctgcttc aatttccaga tctacatgat ctactgccgt
     9541 tttttcaaaa ggtgtcccaa tgccatagcg ataggtcagt ccttctgtct gtatcgttgc
     9601 cacttgcaca cctcatttca cgccatgttc tgcaaaattg cctctacgcc ggcatcttca
     9661 ctgataatat gactgtccag cggaatcccc tgctgccgca gttcatacac cagttccgtc
     9721 acctgcggca cgtccagtcc gattgctttc atctgttcca catgggaaaa cacttcctcc
     9781 ggacggtcgt ccagcagaat gtttccgtcg tccatgacga tcacacgatc tgcctgtgct
     9841 gcttcttcca tatagtgggt aatcagcaca atggtaatgc cataggaacg gttcagcatg
     9901 cgaatggtct tcatgacttc catacggcct ttcgggtcca gcatcgctgt gggctcgtcc
     9961 agtacaatgc aggacggacg catggcgata atgccggcaa tggcgattcg ctgtttctgt
    10021 ccgccggaaa gctgattggg agcatgcatg cgatactcgt acatgccgac cgctttcaga
    10081 gcatcgtcca cccgttcccg tatagtttcc ggcggcaacc ccagattttc cggtccgaaa
    10141 gccacgtctt cttcgacgat cgttgccacg atctgattat ccggattctg aaacaccata
    10201 cctacatgct gccggatctc atacagcttt tcttcgtcac aggtatcgat gccggcaatc
    10261 cacatttttc cgccggtggg cagcagaatg gcattcatat gtttcgccag cgtagacttg
    10321 ccgcagccgt tgtgccccag gactgccaca aaagagcctg cttccagtgt cagagtcagg
    10381 tctttcagga tttccggaga agctggtttt ccctcctcgt cggtatagtg aaatgacaga
    10441 tgctctgccg caatcagttt ttccacggta ttttctcctt ttttcaggca atacgggcag
    10501 aaaattcctg ccgtatcttt atttttgccg gaataccagc agcagaagca ccaccagtac
    10561 cgccacaccc gctgcgaaca acccatacgg agcaaaaaag tgcaccattt ctgcgatgcc
    10621 ctccggcggg tccccataca catagcggcg atccagatac tgtatccctg ctgcgaagca
    10681 gtccgccgcc aaagaaagca cccccagaag caacgttccc cattgttttc gacgcataga
    10741 cttccgacag caccgcacaa acagcgttat gcagtgtttc ccttattttt tccagatcgc
    10801 agtagaaccg cagggcaata ccatgccgct ttgttctacc ggcagcccga tctcatcggc
    10861 tgtcaccccg ccgccgaatt tcgggttcag aacgctcccc agcagatacc ccattactgc
    10921 gggagaaagt ccggtggtat agctgttcag caggaagaac agcgggtttt ccgacaacac
    10981 gccggaacag agttctacca gatcgaaaat gttttcttcc agcttccaga cctcaccgcc
    11041 cggacctctg ccgtagctgg gcggatccat aatgatggca tcgtattttc tgccgcggcg
    11101 aatctcccgt gccacaaact tttcgcagtc atcgacgatc cagcgaatgg gcttttccgt
    11161 cagtccagac agtgcggcat tttcccttgc ccactgtacc atgcccttgg atgcgtccac
    11221 gtgacacaca gaagcaccgg cggaggcaca tgctagcgtt gcaccgccgg tataggagaa
    11281 caggttcagg acagagatcg gtctgccagc gttgcgaatc tgctctgcca tccagtccca
    11341 gttcactgcc tgctccggaa ataatccggt atgcttgaag ccggtgggct gaatcttgaa
    11401 tgtcagcccc agcggttcat attccatttc ccaaaacggc ggcagcttct tggaaaacgc
    11461 ccagcttccg ccgccggagg acgagcggct gtaatgtccg tccgccctgt tccatgcggg
    11521 gtgctttttc ggggtcttcc agatgatctg cggatcaggg cgtaccagtg tgacaccatg
    11581 ccagttctcc agcttttcca attgcgaagt gtctaaaatg cgatattgtt tccaaccatt
    11641 tgctactctc aattcgggct actcctttat ctatcctgca ttatgatgcg gcaaaaatct
    11701 tgacctgttc tgtgatttcc tgtgcagcgg cttcgatctg accggcgttt tttccctcta
    11761 ccatgacgcg gatcagcggt tcggtaccgc tttcccgcac gaggattctg ccgttgtcgc
    11821 cgaggatcgc ggtatgcttt tcaatgactg ctgcaatgtc ggcatcgtcc tgccacagtc
    11881 cctttttctg gggctgaatg gtcacattgt acagcacctg cgggaagcac tccatcagac
    11941 ctgccagttc gctcatcttc ttgccggact tcttgagaat ttccagcagc ttgatgccgg
    12001 acagttcgcc gtcgccggta gtggtttcgt ccagaaaaat gatgtgacca ctctgttcgc
    12061 cgccgatgtt atagccgccg gagagcattt cctccagcac ataacggtcg ccgaccttgg
    12121 tcgcctttgt ggcaatctgg tttgccttgg caaagtggaa gaagcccaga tttgtcatca
    12181 cagtcactac ggcagtattc tttttcagct tgccctcctg ctgcaatgcg tgggcacaga
    12241 tagcaatgat cttatcgccg tccaccagtt tgccgttctc gtccaccgcc agacaacggt
    12301 ctgcatcacc gtcaaatgcc agacccagat cgcactcgtg ggtctttaca tattccatca
    12361 ggttttccat gtgggtggag ccgcagttct tattgatatt ggtgccgtcc ggtgatgcac
    12421 tgagcatatg tacctctgca cccagacgag tgaacaagga ttctgccgtt gcggaggaac
    12481 tgccgttggc acagtccaga accacacgca tgccggtaca gtctgcggca atacagcttg
    12541 ccagataaga aatatagtct tccttggcgt tagcgtattc cgaaatggca ccgactgcgg
    12601 taccggactg caatgtgatc tgctccggat gatccagaat cagtgcttcg atctctgctt
    12661 cgatcgcatc cggcagctta taaccggtag cggcaaacag cttgatgccg ttgaattcca
    12721 cactgttgtg tgatgcggag atcatcacgc cggcatctgc accgtgtgtc cgcaccagat
    12781 atgccacagc cggtgtgggg acaacgccca gctttaccgc ttctgctcct accgaacaaa
    12841 tgccggcaca aagagccgct tccagaatat ctgaggaaat gcgggtgtcc ttgccgatga
    12901 aaatcttcgc acggtgcttt gcctcttttg tcagcaccaa tgcagcggca cggccgatct
    12961 gcattgccat ttcacatgtc agttctgtaa ttgcgacacc tctcgcaccg tctgtcccaa
    13021 acaatctacc catgattctt atgttccttt catacatacg gagatcactc cgatgttttt
    13081 gtcaatgatt tgatattttt aggcaacacc gcacaaaatg cgattgcacg caaagccgtc
    13141 aggcaagctt tgtacagtgt tgctttaaga ttcttctgtg gaaaatttca tctgaccgtc
    13201 cataccgccg ccggaatccg gctgttgata gcccagatgc tgatacgcca atgcagtagc
    13261 acagcgtccc cgcggcgttc tcgccagaaa gcccagctgc atcaggtacg gctcgcacac
    13321 gtcttccagt gtgaccgttt cttcacccag tgcagaagcc agcgtttcca gtcctaccgg
    13381 tccgccgctg tagcccttga tgatcatctc cagcatgcgg cggtcattgc tgtccagtcc
    13441 cagttcgtcg atctccagac gatccagtgc catgtgggcg atttcctgtg tgatctcacc
    13501 gttgcccatg acatcggcaa aatcccgcac ccgcttgagc aggcggttgg caatacgggg
    13561 tgtccctctg gcacgccctg caatttccat tgccccgtca atggagcacg ggatcttcag
    13621 cagcattgca ctccggcgga caatgtccgt cagctgttct gtggtataca gttccagccg
    13681 cagaattacg ccgaagcggt cacgcagcgg gctggaaagc tgccccgctc tggtggttgc
    13741 ccctaccagt gtaaacgggt tcagatccac ccgtagcgaa cgtgctgacg gcccttttcc
    13801 catgataata tcaatggcat ggtcttccag tgccggatac aggatttcct ctacctgacg
    13861 gttcaggcga tggatctcat cgataaacag cacgtcattc ggctgcaaat tggtcaggat
    13921 cgccgccaga tcaccggctt tttcaattgc cggaccagtt gtcacacgaa gattcacgcc
    13981 catttcgtgg gcaatgatcg aggacaaggt cgtctttccc agtcccggag gcccgtacag
    14041 cagcacgtga tccaaagggt ctccacgccg cttggctgcc tcgatataga ctgccagatt
    14101 ttccttgact ttatcctgtc cgatatattc caccagcgtt tgcggacgga gtgtcacttc
    14161 tgcttcgcgg tcttcttcca cttcgcgggg tgtcaccata cgctcctcaa actgcatatc
    14221 actggtttca atcattttgg ttgattccct ctctctcaaa agttacgctg ttttccgaat
    14281 tcctgtaacg ccagccgaat caggtcggag gacgacaggg acggatccag ctttgccacg
    14341 atcggtgcca cttcgctctg ggtatagccc agtgtgacaa gagcttcgat agcttcgccc
    14401 acattgccgc cggactgggt cgccgccgca atctctcctg caccggcagt gccggagagc
    14461 agcccggtgg catcgccggc gatcttatcc ttgagttcca tgacgattcg ctgggcagac
    14521 tttgccccga tgcccttggc acgtgtcagt gccttgctgt caccggaagc cactgtcaac
    14581 aggaactgat ccggtgtcag atccgacaga atggaaaggg ctgccttcgg tcccacgccg
    14641 gaaaccgagg tcagaagctg aaagcactgc aattcctgct gtgtggaaaa gccaaataat
    14701 tctacggcat cttcccgcac gctcatgacc gtatacagca gaacggtttc accgagattt
    14761 cccacctgcc ccagtgtatg cagagtggta cggcaggcat accctacgcc gccgcattcc
    14821 accactgcca ttgccggttc tttggcgatc agttttccat gtacactata tatcatattg
    14881 tatctcctgc tttgatgatt ctcttttgca gctgcacgcc ggtcgcacaa tgtccgtggc
    14941 aaatcgcaag ggcaagggca tcggcggcat cgtccggctt cgggatctgt gccagcttca
    15001 gaattcgcct tgtcatatcc atgacctgcc gcttttccgc ctttccgtag cccacgaccg
    15061 cctgcttgac ttgcaggggt gtgtactcat acatcggaat ctggtgctgc tccgcacaga
    15121 ggacgataac accgcgtgcc tgtgccacat cgatcaccgt tttctggttg gaagtaaagt
    15181 acagccgttc aatcgcgata cagtccggct gaaaccgttt cagcaaaaaa tccagatcgt
    15241 catagatcgc cgtcagacgt gctgcaaacc gtgtgccggc ttctgtggta attgccccgt
    15301 attccagcnn nnnnnnnntc cgccccgtga taggacacca ctccaaagcc tacaatggca
    15361 tagcccggat cgattcctaa aatccgcatt tccagccctc ctgtttgcag gtcagtgcct
    15421 gcctcgtttc acacgtacat ttcattatag cacaaaatga ttgctgtttt caatagctat
    15481 tcaaaatttt atcttgattt ttgaaaaata attgtgtata atggaagtta gcatactttg
    15541 cacacggtag cagaagttgt tctcacagaa agaaacctcc cgcaaatttc aagggcaaca
    15601 ccgcatctgc accgaaaatc ccttcacaga aaggaataca acatggaatt acaggaaatc
    15661 cgcaatgaga tcgaccggct ggacaaacag atccagtctt tatttgaaga acgcatgctt
    15721 ctctgccaag atgttgcaag atataaaaaa gcacacaaca tgccggtttt tcaggcagga
    15781 cgggaacagc agatcctcga ccgcgtagaa cagaatgcca aagaggggct gggactggct
    15841 tcccgcacca tgttcgccaa catgatgagc atcagctccc agttacagca gaaaatgctg
    15901 gtcgctgccg aagatgcccc caatttcaaa acgccccgca tgaaggaagc cgtccgtgtc
    15961 ggctgtcagg gcaccgtggg tgcaaactcc gaaaccgccg cacaggcatt ctttccggaa
    16021 cggcacattc agttctatcc gacctttgag caggtctttg aagcagtaga atccggcgaa
    16081 ctggaatacg gcgttctgcc aatttacaac agcacctccg gaacggttac acagactgta
    16141 gacctgatgg gaaagtacag tttctttatc accgcaatga atcaggtaga agtgatgcac
    16201 tgtctggctg ccctgccgga tgctaaaatg gagaacatca ccgaagtgta ttcccatccg
    16261 caggcactct ctcagtgcag cgatttctta gatgcacgtt ctctccacag ccacgactac
    16321 agcaacaccg ccactgctgc caaaatggtg gcagaatccg gtgacaagac cattgccgcg
    16381 atctgttccg aagactgtgc caagcacaac cgcctacaga ttctggcttc ccgtatttcc
    16441 aatgttgtcc cgaacttcac tcagttcatc tgcatcacca aggacatgca ggtggcggaa
    16501 aatgcgtctg tgatttccgt gatgctgtcc atttccaacg agcccggcag cctgagccga
    16561 atcctgaaca aattcttcct gtatggactg gacatgacca aaat
//
LOCUS       DAWBGR010000038        16018 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1205_length_16018_cov_3.724363, whole genome shotgun sequence.
ACCESSION   DAWBGR010000038 DAWBGR010000000
VERSION     DAWBGR010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16018)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16018)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16018
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1205_length_16018_cov_3.724363"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3797 a         4395 c         4000 g         3826 t
ORIGIN      
        1 cggaaacgac acaaacaatg cctcacatgc ggcatgctgt aagatctgtc cggcttcctc
       61 tgtcagatgt gcctgctcat gttcccctgt gggctggatc agcacacaga caggcgaatc
      121 tgcggcggcg atccggcaga tttttccggc aatttcaatg gtttcccacg tcatatgaac
      181 tcctcctgtt ccattttttc tattatagca tatctttgca aaagttgcaa tgctatttca
      241 gctatctgta tgatattctg aggcaatacc gcaccaagat tgtgcggtat tgcctcagaa
      301 tttctgcaac aatataggaa acaaatttgc aacaaaactt ttgcaccttc cccgccttga
      361 caaatacccc ttaggggtat ataatactgg tagggggtat atccccgata caccgcaaat
      421 acagaaagga gcctatccgc tatggaacat acagaaaaac agggatgtcc ctgttgtcac
      481 aagacaaagg aacgctcgca ggaggaatac accaaactga tccaccgtct gaaccgcatc
      541 gaagggcaga ttcggggcat tcggggcatg gtggagaaaa acgcctattg tccggatatt
      601 ctggtacagg tcgccgccgc caacgctgcc ctgaacgcct tcaataagga actgcttgcc
      661 aaccatatcc gcacctgtgt ggcagatgat attcgtgccg gcaaggacga agtcatcgac
      721 gaattggtga ttacactgca aaaattgatg aagtaggagg ttttcaatca atggaacaat
      781 acaatgttac gggcatgagc tgtgccgcgt gcagtgcccg tgtggaaaaa gcggtttcca
      841 aggtggacgg tgtcaccagc tgctctgtca gcctgctgac caactccatg ggagtcgagg
      901 gcacagcttc tccggaagca attatcgatg ccgtccagaa agccggttac ggtgcttccc
      961 gcaagggcgg ctctgacgaa gcagcggctc cggacagtga tgccctcgcc gaccgcgaaa
     1021 caccggtgct gaagcggcgg ctgatcgcct ctgtgggatt tctggcagtg ctcatgtata
     1081 tctctatggg acatatgatg tggggatttc ccctgccccc gttcttcaac ggcaaccatg
     1141 tggcaatggg tctggtacag atgctgctta ccattatcat catggtcatc aaccggaaat
     1201 tcttcgtcag cggattccag agcctgctcc accgtgcccc gaatatggac acactggtgg
     1261 cactgggttc cggtgcggca ttcgtataca gtacctacgc cctgtttgcc atgacagatg
     1321 cccaggtcaa gggcgacatg gacgcagtga tgagttatat gcacgaattc tactttgaat
     1381 ccgccgctat gattttgaca ctgattaccg tgggaaaaat gctggaggca cactccaagg
     1441 gcaaaacgac caatgctctg aaagggctga tgaacctctc cccgaaaacc gccgtgctgc
     1501 tccggaatgg tgaggaagtc acggttccgg tggagcaggt gcaccgcggc gaacagtttg
     1561 ttgtccgccc cggcgagagc attccggtgg acggtgtcat tgtcgagggc accagtgccg
     1621 tcaacgaatc tgccctcacc ggtgagagca ttccggtgga caaggcagtc ggcgacaatg
     1681 tttcagctgc cacgatcaac cagtccggtt ttctccggtg tgaagccaca cgtgtgggag
     1741 aagataccac gctttcccag atcattcgca tggtcagcga tgccgctgcc accaaggctc
     1801 ccattgccaa gatcgcagac aaggtttccg gtgtgtttgt accggctgtc attgccattg
     1861 ctgttgtcac aattgtggta tggctgctgg tgggacaaac tgtgggatat gctctggcac
     1921 ggggcatttc cgtgctggtc atcagttgcc cctgtgcact ggggcttgct acaccagttg
     1981 ccattatggt gggaaacggt gtcggagcca agcacggaat cctgttcaaa accgcggttt
     2041 ctctggagga agccggcaag gttcagatcg tagcactgga caagaccggt acgatcacct
     2101 gcggcgaacc ccgcgttaca gatattctcc ctgcggcaga tgtcaccgaa caggaactgc
     2161 tctccgccgc aactgcattg gagcagaaaa gcgaacaccc gcttgcaaaa gcgatcttac
     2221 aatatagcga agaacaccag attcccgctt cgccggtcac tgaatttcag gctctggtgg
     2281 gaaacggttt gacagcaaag tggaacgggc aagtgctcta cggcggcaat gccggctgta
     2341 tcgcccaggc gacgaccatt ccggaggaag tacagcagca ggcggaaatg ctggcgaaag
     2401 ccggaaaaac accgctgttc tttgcccgcg ataaccagct tctgggaatc atcgccgtgg
     2461 cagacgtgct gaaagaggaa agtccggagg cgattcgcca gctgcaaaac atgggcattc
     2521 aggtggtcat gctcaccggc gacaatgaac agaccgccag ggcgatcgga aaacaggctg
     2581 gtgtcgatca tgtcattgcc gacgtactgc cggacggcaa ggaagccgtc attcggaagc
     2641 tgtccgcact gggcaaagtg gcaatggtcg gcgacggcat caacgatgca ccggcactga
     2701 cacgtgcaga catcggcatt gccattggtg ccggtaccga tgttgccatt gatgcagcag
     2761 atgttgtact gatgaaaagt cggctgacag acgtgccggc tgccattcgt ctgagccgtg
     2821 cggcactgcg gaacattcac gaaaacctgt tctgggcatt tatctataat gtgatcggca
     2881 ttccgctggc agccggtgtc tggatcccgc tgttgggctg gaagctgaat cccatgttcg
     2941 gagccgctgc catgagcctg tccagcttct gcgttgtcac taatgctctg cggctgaacc
     3001 tgttccagct gaaccgttcc gacagggaca aaaagctgaa acacgctgtg gcagccattc
     3061 agctggaaca gcagactggg gcaacgggaa aaaactccgg catgcaggtc acactgcaca
     3121 tcaagggtat gatgtgcagt cactgtgaag caacggtgaa aaagtgtctg gaggcatttc
     3181 cggaagtgga cgaagccgtt gtcagccaca ccgagggaac tgctgtggta tctctgaatg
     3241 caccggcaga tctggctgcc ctgaaaaaag ctgtgacgga acaagggtat aaagtgttga
     3301 aataacgtaa ggcaataccg cacaaagctt gtgcggtatt gcctttcata aaacctgtcg
     3361 taagaactta tcgtgctttt acgccttagc ccctacgatt gcctgcattt cgatttccac
     3421 cagctgtgtg ggacggttca gttccttgat accgatccat gtgcagcagg gtctgctttc
     3481 cttgaagtac tcggaataag cacgggtgat ctcgttgttg taggaagact tggtggcaaa
     3541 gatgttcacc ttcacaactt cttcgcggga aacgccgttt tcttccagca gaccgaccag
     3601 cttgttaaag atgtactttg cctgttcgta gggatcaccc tcaccgtaaa ccgtgccctc
     3661 cggtgtcacc gatgtggtac cggaaacgta cacgaacgga ccagctttca cgatacggga
     3721 atagcctgcc acgttctcat aagcggagcc gcccttgatg ttcactctct caaaatccat
     3781 tgcaatttcc tccttgccgg aaactttccg aaacacgccg ttttgcggtt tcaccagttc
     3841 ctttgatgct tctattgtac cacggttttt tccgaaataa ttgaacaatt cggactttgc
     3901 ggcagatgcg gaaaaagaaa tgacagctgg cagcagattg acatttttcc agtaatatgt
     3961 tacaatgtag gcatcaaccg caattttcta aaggcagcac cgtgcaaagt tgccagacgc
     4021 tctttgtgcg gtattgcctc aagggaactt tccctgcaac caaaggagga aaaagcacct
     4081 ttatgctgga attaaaagga atcaaaaaag actatctcgc cggagaaaac accgtccatg
     4141 ctctgaaagg cattgacctg cgattccgac ggaacgaatt cgtttccatt ctgggaccgt
     4201 ccggctgcgg caagactacc atgctgaaca ttatcggcgg gctggatcag tacaccgagg
     4261 gcgacctcat tatcaacgga cgctccacca aggatttcaa agaccgcgac tgggacacct
     4321 atcgaaatca ctcgatcggc ttcgtgttcc agagttataa cctgatcccc caccagactg
     4381 tgctgcaaaa cgtggaactg gctctgacgc tgtccggtgt gtccaaggca gaacgccgga
     4441 agcgtgccaa aaaagcgttg gaagatgtgg ggctgggcaa ccagctttcc aaaaagccca
     4501 gtgagatgtc cggcggacag atgcagcgtg tggcaatcgc acgggcactg gtcaacaatc
     4561 cggacatcat tctggcggac gagcctaccg gtgctctgga tacagaaacc agcattcagg
     4621 tcatggagat cctcaaggag atctccaggg atcgcctgat cgtcatggtc acacacaatc
     4681 cggaactggc agagcagtat tcctcccgca tcattcgtgt cctggacggt aagctgactg
     4741 acgactccag accgctgacg gaggaagaat accagcggga attacaggca gacaaggcgg
     4801 aaaacgaaaa cggcaaaaag ctgaaaaagc cctctatgtc tttcggcact tcattctccc
     4861 tgtccctgaa aaacctgttc accaaaaagg gcagaaccat gctgacttct ttcgcaggaa
     4921 gcatcggcat cattggcatt gcactggtgc tctctatttc gcagggcttc aacacctata
     4981 tcaacaccat tcaggaagaa accctgtcct cttatcccct gacgattcag aaaaccaaca
     5041 ccgacctgag tgccctgatg gaaaccttta tgggctatgc cacctcggac agcacccaca
     5101 gcaatgatgc ggtgtacaag aagtcggcga tctacgatat gatggacgct ctgaacaaca
     5161 tggaaacttc cgaaaacgac ctgaaaagct tcaagtccta tattgaatcg aaacggaaag
     5221 acagcgaaag caacctgcac agggcagtca acggcgtaca gtattcctac gatctggaca
     5281 tgctgatcta caccaaaaat gtggacggca ccattatcca ctccgacacc gaagaactga
     5341 tgcaggaaat ggtactggaa cacatgggca ttgatatgca ggcaatgacc gatgccagca
     5401 cttccatgtt cggcgaagac gctacctcga tgatgtccat gggtatgggc ggttcgtcca
     5461 tgaatctgtg gcaggaactt ctccccggcg acaacggcaa gcccatcaac gacctgctga
     5521 aaaagcagta cgatgtggtc tacggctcat ggccgaacag ctacgacgag atcgtactgg
     5581 tgctggacga aaacaacgaa ctggacgaca tgacattata cgccctcggt ctggaaccga
     5641 aagaagatgt ggacagcatc atggaagcgg cagtgaatgg caaggaactg caaaaggaca
     5701 acaaaagctg gtcctatgcc gacatttgca gcatggactt ccgcacgatc ctgaattccg
     5761 actgctaccg ctatgacgaa agcacagggc tgtacaccga cctgcgggac accgaagccg
     5821 gcttgcagta cctctacgac aacggtctga aattaaaagt gactggcatt atccgcccca
     5881 atgaagatac caccgctccg atgctgtccg gctccattgc ctataccagc gacctgacca
     5941 agtatatcat tgaacacagc aaggaatccg atgcgatcac tgcccagaag gacagtaaga
     6001 acaccgatat tttcaccggc ctgccgttca aggatacctc cggcagcacc tctgatgcgg
     6061 acaagcagac tgcctttctg gaatatctga acgaactgga cgacggcgga aaagcacagg
     6121 catatgtaca gataatgagc attcccaccg aggaacagct gaacgattcc gtacagcagg
     6181 caatgcaggg catgagccgc agcgatatgg aagcggcaat gctgcagggc atgacacagc
     6241 agatgagcat gagcgaaagc gatgtgcagt cctacctgga atccatgagt gacgacgaaa
     6301 tcaccgacac attcacccag atgatgcagc agcaagtcaa ggcacagtat gcccagcagg
     6361 tacagcagaa aatggctgcc atgcagccgg cagaactggt aggagctttg aaccagctgc
     6421 tccccaccct gaccacggaa cagtgtgcga attactatga cgaaatgatg cagttctccg
     6481 attccaccta tgaggacaac ctgaaagcac tgggggatat tgatctggac gaccctgcat
     6541 cgatcaatct ctatgcagca acctttgagg acaaggatgt gatcgaagat gccattgcag
     6601 actacaacga gggcaaggac gaaatggcac agatccagta taccgactat gtggggctga
     6661 tgatgtcctc tgtgacgaag atcatcaaca ccattaccta tgtgctgatc gcatttgttg
     6721 ccatttcgct gatcgtttcc tccattatga tcggcgttat cacgttgatc tctgtacagg
     6781 aacgcaccaa ggaaatcggt attctccgtg caatcggtgc ctcgaagcga gatgtttcca
     6841 gcatgttcaa tgcagaaacc attatcattg gatttacctc tggtttgctg ggcatcgtgg
     6901 tcacatatct gctctgcatt ccgatcaacc tgctggtaca ccacctcacc ggcattcaga
     6961 atctgagtgc ggttctcccc gttccggcgg cgatcattct gattgcgatc agcgttctgc
     7021 tgacattgat tgccggattt atcccgtcca agagtgcggc aaagaaagac cctgttgtgg
     7081 cactgcgttc ggaataattc gaggcaatac cacacaaatt ataacaccat aagtaaaaag
     7141 ccataccgca gagcgtttat tctctgggat atggcttttt ggttttctct atatcgcaag
     7201 ctgcgttact gtagcaattc cgccaagtcc tgctccggtg tggtgatcgg ggtgataccg
     7261 tagctgtcta ccagatattg cagcacgtcc ggtgacagaa atgccggcag cgacgggcca
     7321 agccggatat tccggattcc cagatgcagc agtgtcagca gaatgcagac tgctttctgt
     7381 tcataccacg acagcacata ggacaggggc agttcgttga caccgcagcc gaatgcctct
     7441 gccagtgcca ctgcgacctg aatcgcacta taggcatcat tacactgtcc catgtccatg
     7501 attcgcggca gcccatcaat ggtgcccaga tccagatcat ggaaccgata cttgccgcat
     7561 gccagtgtca gcacaatagt gtccggcggt gtctgcttta cgaattctgt gtaatagttc
     7621 cgtcccgcct tggcaccgtc acagccgccg accaagaaga agtgccgaat ctttcctgcc
     7681 ttgacagcgt tcaccacctt gtccgccacc gacagtacag caccgtgtcc gaatccggtc
     7741 gtcagttctg tgccgccgtt gataccggtc tgctgctgcg gtgctgtata gccgcccagt
     7801 tccagtgcct tttcgatgac tggcgtgaag tccttatcct tgccgatgtg taccatttcc
     7861 ggaaagccca ctgcttctgt ggtaaaaaca cggtcggcat agctgggctt cggcggcatc
     7921 aggcagttgg tagtaaacag caccggtgcc ggaatggcat cgaattcttt ctgctggttc
     7981 tgccatgctg ttccgaaatt gcccttgaga tgcggataag ctttcagctt cgggtacgca
     8041 tgtgccggca gcatctcccc gtgtgtgtag acggcaatgc ccttgtcctt ggtctgttcc
     8101 agcagcagct ggaggtcttt caggtcgtgt ccggtaacta caataaacgg tccggcatcg
     8161 atctgcatgg ataccttggt cggctccggt gtgccgtagg tttccgtgtt ggcacgatcc
     8221 agcagtgcca tacagcgaag gttgacttct cccagcttca gcacttccgg cagcagttcc
     8281 tccatggtca gatccagccc gatctgcatc agtgcttcat agaagaactg atttaccgtg
     8341 ttgtctgtat aacccagcac catagcatga taggcatagg ctgccatgcc acgcagtccg
     8401 aacagcagca gcgatttcag cgaacggata ttttcttccg cctgccacag ctgttccatg
     8461 ttgtaatctt cgtttctgcc gcagggcgat gcacagacgg cacaccgggg aatcattctg
     8521 ccctgttccc gatgcacgcg gtcgatttgt gcctgaatcc ggctgtcgtc aaaattgaca
     8581 ttggtcagag tcataaacag gctctcgatc atcaacgcat ctgtcttttc tgtaacaaga
     8641 tcttcattgc cgtccacggc tctcgccagt ccgatcagag caccggtcag ttcgtcctgt
     8701 agcttcgctg tgccggcttt tttgccgcac acgcctgcgg ctccggtaca tgctgtacag
     8761 cctacagtct gttcacactg gtagcaaaac attttctgtt ccatgttatc tctcctcctg
     8821 aatggttccg tctgttccaa tcaccgttac gtgacaagaa atgtcttttc cgcttgccgc
     8881 aatggcacgc tgcactgcct gttcgatacc gccgcagcag ggcacttcca ttcgtgccac
     8941 ggtgacactg cgaatggaat gtgccgcaaa aatcgccgtc agcttttccg catagtccac
     9001 cgcgtccagc ttgggacagc cgatcagagt gattctcccg cggataaatt tttcgtgaaa
     9061 atttccgtag gcataggcgg tacaatctgc cgcgatcagc agatcggcat catcaaaata
     9121 gggtgccatg acacctgcca gcttgatctg caccggccac tgggaaagct gactgggaca
     9181 ggctgctcct gctgcgggag cagtgcttcg ctgaaactgt ttggcacgca ttcccggaca
     9241 gccggaaaag ggctttttct gtgccagctt ctgggcacgc tgcacagctg cttcgtcata
     9301 ggcaggggct tcccgctcct caaaagtgat cgcatcggtg ggacatgccg gcagacagtc
     9361 gcccagaccg tcgcagaaat gttcccgcac cagcactgcc tttccgtcaa tgatgtcgat
     9421 cgcaccctcg tgacacgccc tggcacaggc tccgcagccg ttgcatttgt cacggtcaat
     9481 gtgaatgatt tttcttatca tgtgtattcc tcctcatatc atacgcaaaa gctttgcctt
     9541 tcgatactgt tgccttgact ttctgtatcc attatagtat aataacagca tcttgtatgt
     9601 tgaaatttca acaaaacgga ggttcacatg gagaaaattt ttccaatttt aaagcagtgt
     9661 acgctgtttg ctcacattgc cgaaagcgac ttgtccgcca tgctgcactg tctgcaagtc
     9721 acggtgcagc actatgacaa agggcaaatt gtgatacacg agggcgaaaa ggcagaacga
     9781 tttggcattg tgctgaccgg tgcggtgcag ctgtggcgta tcgactactc cggcaaccgg
     9841 agcattatgg caaacgtgga gcctgcacaa attttcggcg aatcctttgt ctgtgcagaa
     9901 gtggaatcga ttcccataca ggcagtagca tctgcgtcct gcgacgtgat gctgatcgac
     9961 ggcagccgca ttctgcacgc ctgcggcaac ggctgcgaat ttcatcagca ggtgattttt
    10021 catctgctgc aaatcattgc ccgcaaaaat ctggtgtttc accagaagct ggagatcacc
    10081 tccaaacgta ccacacggga aaagctgctg acctatctca atcagcaagc ccagcggcac
    10141 cggtgcagca cgtttaccat tccgtttgac cggcaggaac tggcagattt tctggaagtg
    10201 gaccgcagcg ggctttcctc agaaatcagc aagctgcgaa aagagggcgt gttggaaagc
    10261 gaacggagca cgttccgact gctgtgacca gataaaaaag ctgtgcaggt tatcaaaaac
    10321 ctgcacagct tttttgcaaa tataaaggag atgatattgt ctgtgttttc cttattcctt
    10381 taccagttct gcacccagag ccgcacactt ttccagtgct tcatcgtccg gagtttcgtt
    10441 gacgatcaga ccttcgccgc caaccagcgt tgcaccgtca ttcttagcgc gttcttccca
    10501 gttccgcatc cattcgccgt cgccccagcc ataggagcca aacagagcga tggtctttcc
    10561 ggaaaggtcg ccttcgatcg aagcgaagaa cggttcgaat tcgtcttctt ccagaacctc
    10621 gtcgcccatt gcagaacagc caaatgcgat acggtcatag tctgcaatgc tgccgctgaa
    10681 gtcagatact gcgaacagtt ctgcacctgc tgcatctgca attgcctgtg ccatctgttc
    10741 agtattgcct gtgccactcc aataaattac tgctgctttc atgctttttt cctcctaaaa
    10801 attctgctga ctttcgccgg caaccggtga cattgcaaag ctttttgcaa tattaccctt
    10861 atgatatttt cagagcctgc aaaatattac agcccttact caactttgca cagagaaata
    10921 ctctgcactt ctcatagctg gcaaatgtcc ggcgtgcctg ctccacgttt ccgtacactt
    10981 tccaacaacc gcacagcttg cagttttccc cctgatgcgt gataaagccg tccacatctt
    11041 ccaaagggta ccccagaaaa attccgatct catggggaaa ttccggcttc tccgcaatgc
    11101 ggttctgcaa ccgcatcaga catgccgcca gatccatatc agcggtgtaa ccatatcgca
    11161 gcagaaaacg ccggatttcc ggctgttcca gccgtttttg cagcagcttc gggcgataca
    11221 gcagtaccag ctgccgcttc tgacagcggc acatgatctg catctgcaat cccccgcgga
    11281 ctgcctgtgc attaaacgct gccgcatacg ccggaatgtc aaattcctct cgggacaagg
    11341 acaccagact ggcacacttg atgcccagca gtgtcggtgc cgtaaaatgg gcaagctttt
    11401 tttcgaatgt tctgcactct cttcacctct gatttgcaat gctgtacgtt ttgtatatct
    11461 caggaaatac acaagggtta gaaaaagcta actactttcc cgaaaaaaca gagctttttc
    11521 ccgctctgca tagttagttt tcactaacag tagttagtat agcataactc aaaccgtttg
    11581 tcaagtgttt tttgcaaaaa aaatacggaa catgccacag ggcacattcc gcattcacac
    11641 ataaagcctt tatatagcgt ttctcttagt atctgggttc aaacttggaa caggagttca
    11701 acgcctgtgt cgttcttgca gtctggtagc aggtctgctt ttctgtcatt tccacttgga
    11761 aatagtcctt cgttttcatc tggatcattg tgctgcccat tgcaccgttc caatgacggc
    11821 acaacacgca tttttttgcc gttttcaaaa gtgtgtactt tgccatattc ctcacctttc
    11881 cgtccggaat gctccggtgt ttccgatatt cctgcaagtc gtctgccgca gctatgtact
    11941 cgctgtaagg gattctcctt acatactcta tcatacactt catatcggac aggtgactgt
    12001 ctgttcaaaa agaatttaca acattttgca tttttcgaac agcaaaaacg tgttcccgca
    12061 aagatgcaat gcacgatttt ccgtacaatc attttgtggg aacacgttct acagtttagc
    12121 tttgtacggt gttgcctcaa ttaaaccgtt cgtcaatgac acgggcagtc tttttggtgc
    12181 tgcggggcag tacgccaact tctaccactt tgacgatcgg tgtgaagccg atacggctct
    12241 tgacctgctc tgcaatgcgg cggctcaagt ctgcgaagtc cacatcaccg gtggattcca
    12301 catagattcg catggtgtcc ttgccgtcca gatgagagat gcgaatctgg tactcgctgg
    12361 aaatttcatc aaatgtgctc agaatctcct cgatctggct ggggaatacg tttacgccct
    12421 tgatcttcat catgtcgtcg gttctgccca taatgatgtc gatacgcggg aacttgctgc
    12481 cgcaggcaca ggtgcccgga atgatgcggg acagatcgtg tgtacgatag cgaatcagcg
    12541 gtgcaccctg cttgaccaat gttgtgatga cgatttcgcc gacctcgccg tccggcagat
    12601 tttcgccggt gaccggatcg attatctcca gatagatata gtcgttccag tagtgcatac
    12661 cgcagttttc cttgcagctg atgccgattc ccggaccata gatttctgtc agaccgtaaa
    12721 tgtcatacag ttcaatgccc agttcgccgg aaatccggtc acgcattttc tgactccaac
    12781 gctccgaacc gataacaccc tttttcagat gaatcttgtc cctgatgcca cgcttgccaa
    12841 tttcctccgc cagcagcaac gcataagaag atgtggaaca cagcacggtg ctctccatat
    12901 ccatcatcat ttgcagctgc ttctcggtgt tgcccggtcc catgggaatg accattgctc
    12961 caagcttttc cgcaccgttc tggaaaccaa taccggctgt ccacagaccg tagcccggtg
    13021 tgatctgaat ccggtctttt ttggtgatgc cggcagtttc ataacaacgc ttgaactgga
    13081 ttgcccagtc gtctacgtcc ttggcggtat acgggataat gactggtgtg cccgtagtac
    13141 cggaggacga gtggatccgg acgacttctt cttccggtac tgccatcagt cccagcggat
    13201 acgcgtcccg cagatctttc ttttccgaaa acggcagctt cgcgaattcc tccggcgtgt
    13261 gcacttctgt catgccggca gcttccagct ttttgccgta aaagctgttt gagcgtatca
    13321 acgcctgcat gcgatcgttg acctgttcca actgtgtttg tgtaatcttc attgtcaaaa
    13381 cctcttattc ttcaatatgc agtgctttcc ggttcaattc ccgaaactgc ggctttacag
    13441 tacgttccat ggtttccgtc acttcttcca atgtcaaagg cagtaccccg ctgcggagta
    13501 cggctcccag catgaccatg ttcagcactt tcggggaacc gatcttccgg caggcagcat
    13561 cgccgtccag catcgtcaga ttgccggcat gcttctgcaa ataggcaatc attgccgcgg
    13621 catcataggc accgccgccc agcgttgctg ttaccggctg aatcgggtgc gtgttgagaa
    13681 caactgttcc ctccgatttc agataaggca gcatgcggac agcttccgcc ggctcaaagc
    13741 cgatcagaag atctgctgtg cccttgggga acatggggct gtacagctcc tctcccatgc
    13801 ggagaaagct gaacacgctg ccgccctttt gtgccatgcc aatggtttcc gcactcatta
    13861 ccggaatgcc ctttgccatt gccgccgctg ccagcagttt ggaagtcagg acaatgccct
    13921 gtcctccgac accgcagaga atgatattct tacgcatgac aagcacctcc tattgccgca
    13981 gtggggcaga cctgtgcaca cagtccgcag ccggtacaaa gtgcctgttc gatctgcacc
    14041 ttgccgtcct gtagcatgat cgccggacag cccagtgtcc ggatacattt cttacactgt
    14101 atacatttgc tttcttcgat ctgtgccggt ttgccggact tgatgagtac ggcacagggc
    14161 gacttgaaga tgatcgcctt tacaccgtcc tgcacagcca cccgttttac gcagtcgatt
    14221 gcctcctgca agtgcagcgg gtttacggtt tctacggttt tcacaccgat gccgtggagc
    14281 gtgtcctcga tgctcacctt gtccaccact tgccccatca tggtttttcc ggtgccgggg
    14341 tgaggctgat gtccggtcat tgctgtggtg gaattgtcca gcaccaccag tgtcatattt
    14401 gcctgattgt ataccgcgtt aaccatgccg gtaattgccg acgcaaaaaa tgtggaatcc
    14461 cccacgaaag caaaacaagt ggtatccggt tcgatttttc caatgccctg cgtcatgttg
    14521 acaccggcac ccatgcacag acaggtatcc accatgtcca gcggcatggc gttgcccagc
    14581 gtatagcagc caatatctcc gcagaatacg ctctttttgc ctttcatcgc ttcttttacg
    14641 gcaaagaacg aggctctgtg cggacagccg gcacagagca ccggcggacg taccggcagt
    14701 tcaggtggct ggtctgctgt gttctgctcc ggcggctgcc agcccagaaa ggttgccaaa
    14761 tctgccgcca cgctgtcgca ggaattctct ccggcaagag ccacgtcctc tgtcagcttg
    14821 ccgcggatac gggtgggcag gtggtacttg ccgcagaggt acaccagttc ctgttcaatg
    14881 acaggatcca gttcctccag acacagcacc tcgtccagcc cctgcaaaaa ctccactgcc
    14941 agctgttccg gaaacgggaa cggtgttgcc actttcagca gccgcggtgc agctttgccg
    15001 tgcagggctt ccatggtata ggaaaagctg atgccatggg tggcaatgcc cttccgacat
    15061 gcggtatctg tttccggata gatccggttg cggggatagc cggaaaagac actttgcagt
    15121 tcggtattcc gcttttccat acggatatga ttctgatagg acagcttggg aaaaatcacc
    15181 cacttggagg agtctttcac aaacccctcc ggcgagttga cctgatattc tgccgcatct
    15241 ttcaccgtga tcgaagcgta gccatgacag actctggtcg tcgggcggaa cagcaccggt
    15301 gtgtggtaac gctccgaata ggcaaacgcc tcctgaatca tgtcataggc ttcctgcacg
    15361 ccggaggggt cgaagcaggg cagcttggag aacgacgcaa accgccgcgt atcctgttcg
    15421 gtctgcgagg aaatcggccc gggatcgtct gccaccagta tcaccatacc gcctttggca
    15481 ccaatatatg ccaaactcat cagcggatca gaagccacgt tcagccctac ctgcttcatg
    15541 gtcaccatgg ttcgtgcacc gcagtacgcc gcaccggcag ccagttccat tgccgctttt
    15601 tcgtttgtgg accactccac ataaatgctg ccgtcattgt gcttggcagt cgtttccagc
    15661 acctcggtag acggcgttcc gggatagccg gaaaccacgt tcagacctgc cgcaatggca
    15721 cccatggcaa tcgcagcatt tcccattaaa aattctgtat gcatgttaac cttccaatct
    15781 gttttgggaa tcccagtccg ctgggaacgg cttttcgcaa gtcaagatgc acaaaagcct
    15841 gtcttttatt ataacggaat ttgtttgcaa agtcaagtgt tctgcaataa aacaaatccc
    15901 attttattac acgtgcaaca aaaagccgcc atgcacaact gcacagcggc tctctatttc
    15961 aggaaaatta gggaaacgct gcataaagcc cgcctgacgg ctttgcacgc aatctgct
//
LOCUS       DAWBGR010000039        15799 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1218_length_15799_cov_4.646850, whole genome shotgun sequence.
ACCESSION   DAWBGR010000039 DAWBGR010000000
VERSION     DAWBGR010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15799)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15799)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15799
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1218_length_15799_cov_4.646850"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3492 a         4710 c         4006 g         3591 t
ORIGIN      
        1 gattgcgtgc aaagccgtca ggcgggcttt attcagtgtt tccttaaagt aattgtacca
       61 taaatttgcc agacttgcaa gctggtttcc acttttttat attgataaat tgtaaaagtg
      121 aaagattctt tgggcaatgc cgcgtaaatc cggcaggcag tactgtattt gcaaaagtgc
      181 agtcaaaaaa tgcagaaaat ggaaaatgac aggggatttc tctgaaaccg gtaggattat
      241 atacgattgt tgtaattttt tttgcaatag tacttgccac aacgcaaaaa aaatgctata
      301 ataggattgc aaagcacgcg tgaaacaaag cagaagaatt cccttttcct tgttgccgtg
      361 tgcagaacat ttctgccgga atcacttcgg gcaaagaaag gaaaacgtct gccgcctgag
      421 ggcaggcgag tgaatataca tgcgtgcaac tactgcttct gcggaaacga cttctgccgg
      481 ttcgatctgg cggaaacgct tcctttccct gatcagcgtc gggtatctgg ctctgatgtg
      541 ctggttttct tatctggcga tcttttacga attttccgtc accaactccg tgttgttttg
      601 tctgaccctc tgtgtggtat cttttgcggc actcagtgcc atgctgtaca gccggtttca
      661 gatcctgaca cgcctgacgg ggatcctgct gctgccggcg atattgcccc agattttgct
      721 ttgtttcggg caatgggaac tgatcctgcc aattgcagtg acttccctga tcatcttttt
      781 cctgtccggt gcaggggaaa ctgccaagac cgtattcggt gtcatatacc tgttgctgta
      841 tattctgggg tcactggcat ttttcatgct catgtctttt ttcacgccga gtacacagca
      901 gactgtgctg gaaaacggca cttctccctc cggagcatac cggtatgaga tcattcagac
      961 agacgacagc tccggcggca atgtggcagt acacgtagag ccaaatgatc gggacattca
     1021 cctgccgttc ctgacattta tctccaacgg ctatgaccgt accgtctatg aagaacgccc
     1081 cgttccctct gaagtcggca gtgcagagtg gacgacagct tcccgtgcag acatcactgc
     1141 acagctgctg gagatctcca atgatgtaac actggatctg accaaggcac agaagtccgc
     1201 ggtcggtatt ccggcagata cggaaaccgt ctatctgaaa gacctgaccg atgcacagct
     1261 ggagcagctg ggtgtgccgg cagaaaatga cgttctgacg ttctccggaa aggtctgctt
     1321 ccggtcttat attgccgtgc tggaggatta tttcgcaaag gacaaccgcg aaatttcatt
     1381 attcaattaa gttgcgcaaa gcggaatccg ctttccatat accacacctt attattaatc
     1441 aggaggaact actgatgagt gaatttttca acaacattcc gaagattgct tatgaaggca
     1501 aggattccaa gaatccgctg gcattcaagt tttataatcc ggacgaggtt atcgccggca
     1561 agaccatgcg ggaacagctg aagttcgcac tgtcctggtg gcacactatg ggcggcgacg
     1621 gcacagatat gttcggctgc ggcaccacca acaagacatg gggctgtgat gatgtcactg
     1681 cacgtgcaaa ggcaaaggta gaagctgctt tcgagatcat ggacaagctg agcattgact
     1741 actactgctt ccacgacaga gatctctctc cggaatacgg cagtctgggc gaaaccaacg
     1801 aaaagctgag agccatcgtt gacctgtgca aggaaaagca ggacgctacc ggcaagaagc
     1861 tgctgtgggg caccgcaaag tgcttcgatc acccgcgtta tatgcacggt gcaggcactt
     1921 ctcccagtgc ggacgttttc gcatatgctg ctgcacagat caagagtgcc attgatgcca
     1981 ctgtaaagct gggcggtcag ggctatgtat tctggggcgg ccgtgagggc tatgaaaccc
     2041 tgctgaacac caacatgggt ctggaactgg acaacatggc tcgcctgatg catctggctg
     2101 tagactatgc aagaagcatt ggcttcaccg gcgacttcta tgtagaaccg aagccgaagg
     2161 aacccaccaa gcaccagtac gacttcgact ctgcaaccgt tatcggcttc ttacagaagt
     2221 acggtctgga caaggacttc aagctgaaca ttgaagcaaa ccacgcaaca ctggcacagc
     2281 acaccttcca gcacgaactg cgtgtggcaa gaaccaacgg catgttcggt tccatcgatg
     2341 ccaatcaggg cgacccgctg ctgggctggg ataccgacca gttcccgacc aacgtctatg
     2401 atgcagcact gtgcatgtac gaagtactga aggcaggcgg cttcaccaac ggcggtctga
     2461 acttcgacgc caaggcaaga cgcggctcct tcgaactgga ggacatcttc cacagctaca
     2521 ttgcaggtat ggataccttt gcactgggtc tgcgtatcgc ggacaagctg atcaccgacg
     2581 gcagaatcga caagttcgta gaagatcgct atgcaagctg gaataccggc atcggtgcag
     2641 acatcatctc cggcaaggca accatggcag atctggaaaa atacgctctg gaaaagggcg
     2701 aagtgaccgc ttctctgacc agcggccgtc aggaaatgct ggaagcaatc atgaacgaga
     2761 tcatgttcag cctgtaattc cacaaatata tcgaggcagc atcgctgctg aaacgaaaca
     2821 aaagcacatt gacggcatca ggcatagtca atgtgctttt ttgacaggag ggtaattctg
     2881 aatggaaatc caaaacggca gacccgcaga cgaaagcggc agactgccaa aagagatcgc
     2941 cgtatacaac ttactggaac ggctcgacat tccctttgca cgggctgacc atgcggcagc
     3001 ctttaccatg gaagcctgcg aaagtatttc tgcggcactg caaacgcctg tctgcaagaa
     3061 tttgtttctc tgcaaccggc agaaaacgaa cttctatctg ctgctcatgc ccgccgacaa
     3121 gccgtttcgc accaaggaga tcaccagcca gctgggctgt gccagacttt cctttgcatc
     3181 agaagatgcc atggaacagc tgctgcaact gactcccggc tctgccacga tcttcgggct
     3241 gatgcatgac accgaaaacc gcgttcagct tctcgtagac cgcgacctgc tgggagaagc
     3301 cactttcggc tgtcacccct gcataaatac ttctacgatc cgcatgcaga ccagcgacgt
     3361 ctttgacaag ctgacaaccg ccctgcatca cccctacacc atcgtaacac tcaaaggaga
     3421 ataacggtta tggcaaaaga aaaagtcatc aaaaccaatg cgatccgcat gctggacaag
     3481 gcgaaagttc cctatgtaca ccgcagctgg gaacaggcaa ccggacagca caccggcgta
     3541 gaactggcgg cggcactggg acagaatccg gaacaggtat tcaaaacgct ggtcaccgtg
     3601 gcaaagtccg gcgaacacta cgtttttctg gtgccggtgg cggaagaact ggacttgaaa
     3661 aaggcggctg ccgcagtggg agaaaaggca atctccatgt gcaaatccaa ggatttgctt
     3721 gccctgaccg gatacattca cggcggctgt tcccctatcg gcatgaaaaa actgttccgc
     3781 actaccattc atgagtccgc acagaatttt cccacgatct tgtgcagtgc cggcaagatc
     3841 ggacaccagc tggaactgtc actggacagt ttgcagcagg tcgtgccctt tcagctggcg
     3901 gacatcatcg cggagaaagc acaatgagat actgctgtac cgtgttccag cgggtgggaa
     3961 cctgctatca cgaattccgg aagggcagct tccggcaagg ctccagccac tggagttccg
     4021 attctctttt gctgcacgac aacattctac agcgttccgg tttcggaaaa atcttgcagg
     4081 aaacactgcc cgcgtataat cagtacggca tcaccgttgt caactggaca cagtggcagt
     4141 gtatcttaca gcatgccctg gcacagggcg gcggtgcgga gctggtcgca ctggaagtga
     4201 atgaatgggc agaggatacg tttcgggatc acgcggtgtt tacaattttg gggatttgaa
     4261 aggggaaacc gatcatgcaa cacaaaaaga ccacagccat tctgcttgcc gttctggcgg
     4321 cggcatttta cgcggtaaac gttccggttt cgaagctgct gctgaaggag atcagcccta
     4381 catttctggc atcactgctg tatttcggtg ccggcatcgg catcggactg ctgtactgca
     4441 tcaaccccaa aagccaccgc ggcgagaaaa aactgacacg caaagacctg ccctataccc
     4501 tcggcatgat cttgctggat attgccgcac cgattctgct gatgctgggg ctgaaaagtg
     4561 catcttccgc caatgcttct ctgctgggaa actttgaaat cgctgccact acgctgatcg
     4621 ccctgctgct ctttcgggaa aaagtttcca agcggctgtg ggctgccatt ctgcttatca
     4681 ccctgtccag cggcattttg tctttcgagg atatgtccag cctgcacttt tccagaggct
     4741 ccctgctggt gctggcggcg gcagcctgct ggggactgga gaacaactgc acccgcaata
     4801 tttccgacaa aagtactttc gagattgtct ttctcaaggg catcttttcc ggactgggtt
     4861 cgtttctcat tgctctgtgc atcggcgagc cgttccccgg actgcacgct gctgctgcgg
     4921 cactgcttct gggatttgtt gcctacgggc tgagcatttt ctgctacgtc cgttcccaga
     4981 acaccctcgg agccgccaag accagtgcct actatgccat cgcccccttt atcggcacgg
     5041 ccctttcctg catcatctgg cacgatgccc tgacagtgca gtattttctg gcactggcgg
     5101 taatgctgct gggttccgtt ctggtggttc tggacacgct gcaaacggaa cacacccatt
     5161 cccacaccca cacgttcaca cacacccacg acggcgtgac acacacccac accattaccc
     5221 acagccaccc gcaccttcac ggcatgcacg gggaaagtcg ccagcaccgc cacaccatgc
     5281 acacgctgct gcgttcacac aactcattgc agcaccggta acacctgttc gtctttttcc
     5341 cgcagcagcc agaacaccag cccgtacagc cccagagcga ttcccagaaa cagcatgatt
     5401 ccctgcggcg tttcccgcaa agtcgggcag aaccattccg gcagagaagc tgccgtaaac
     5461 gaaaatcccg cggcaaacac cggctttccg ataagctttc cgaatcggaa ccggaggttt
     5521 gccgttttta ctgccatacg cagacgattg cagtttccga acacgttgct ggtatagtac
     5581 gatgccacga tgccgtaaaa tccgaacagc ggaatgcaga ccagcaccac acagatgcgg
     5641 atcacgtact ccgccagata attcagggaa gaaaaattct gcctgcccat gcctttaatc
     5701 agtgcttcca gcacgatttc cagatagata aacggcacca ccggtgccag aatccgaatc
     5761 atcttccccg acagttctcc gccgtccatg caccgcccga tcacgccgcc gaatttcagc
     5821 aggactgcac tgatcagcac cgcaaaaatc agtgtcagtg cgatcacctg ttctgtcagc
     5881 cgacagacac gttcccgatt gccagcggca ttggcacggg cgacttccgg aatcaggatt
     5941 ccggacaggg cacagagaat ggtggacgga aaaaacagca ccggaatcac aatgccctca
     6001 aaagtgccga actgccggag ggcattgctg gcactgtccc cgaactgccg cagtgtcacg
     6061 ggaatcaggg catcattggc tgtgctaagt ccggcagtca gacagccgcc cactgccaca
     6121 ggaactgcca gccggatata ccgccacagg gacaggctgc acgttccgcc gcagggttcc
     6181 cgcttctgaa agtacagcac tgtcagtgtg accgccgtaa acagtgtgcc acacgccatg
     6241 ctgcacacca gatccgtgca gacctgttcc ggtctggtgt ccccacgcca caggagcagt
     6301 ccggtgagga ttcccgcacg gagcagaaat tccgccacat cgcaggcggc tgtcactgtc
     6361 acgcggcaca ctgcattgaa atagcctttc aggcaggcac tcagactgcc cagcggcaga
     6421 agcagtgcca tgagccgcac cgcatgtgcc atggaatcgc tctgcaaaaa ctgctgactt
     6481 agaggttggg caaatatgca gactcctgct gtcaccggca gggacagcat catgcaaaag
     6541 ctgataccat gccgcaggac acgtgccgga cagccgcagg gctttcccag ttcctcggac
     6601 acaaaacggc tggcacagag catagcattt ccgccggcga ccattgcggc aaggttgaaa
     6661 aaagaacctg ccagtgccag cgttcccacc gttgccgccc ccacacgccg cgtcatccac
     6721 gcgttgaacc acaacgccag acactccaga aaaaattgta tcacggtcat ctgaatggta
     6781 tcccgaatta cactgcgttt tcgcatctgt atgcctcctt caaggcaaca ccgcacaaag
     6841 catgtgcgta agatgcccta aggaaacata tgcaccggac aaaaagaaca tgccccgcac
     6901 ggattggttt cccgtgcggg gcatgcgttt ttttatttgt acgctagaat caatgcttac
     6961 acaaatctgc tcactcactg ctgtacccca gccagaaact gctgcaactc ctccagaaac
     7021 attttcccgt acttctgtgc cttgatgaca cccacaccgg aaacctgccg catctcgctg
     7081 aggttctggg gctttttccg gcacatgtcc cgcagcgtgg catcggaaaa gatgaaatac
     7141 ggcggtattc cctcgtgctt tgcctgtgcc gcccgcagct gccgcagctt ctgaaacagg
     7201 tcgttgtccg ccgcggacag cgtaggatct tttgttccca ccggagctgc cagcttctcc
     7261 cgcaccaccg gcacacgccg catgaccgtt ttctggtcgc gaatgatctc caccgagccg
     7321 gcagtcagca ccaattcctg atactcctga aactgccgca gtgccagatc ttcccgctgg
     7381 atcagttcat cgatcagctg catcacttcc cgccgcgaca gcttttcgat aatgccgtag
     7441 gtggaaagct gatccagccg catgcggaga agcgattcct ttttgctgcc catcagcaca
     7501 tcggcagtca tagtgcggct caggtgatag ccgcctttct gggcacgata cacgcaggag
     7561 atgatctttt tcgccgccgt ggtggcatct tcttcccgat aattggtcag acagcaggaa
     7621 caattcccgc agctgtccgg tgaatggtcg ccgaaatatt gcagcatgcg gtgccgcaga
     7681 caggtggtag aggtggcata gaacgtcatc tgtttcagcc gttccttgcc ctgctccagc
     7741 aggaactgcc gtgtttcctc gtcctccacc tctgtggttt cccgactgcg ttgcagcagg
     7801 aaatcgttgg tacggacatc ttttccgctg tacagcagaa tgcactgtgc cggcgagcca
     7861 tcccgtccgg cacgacctgc ctcctgatag tagctctcca gatccatggg catgttatag
     7921 tgaatgacaa acgacacgtt ggacttgtca atacccatgc cgaacgcgtt agtcgccacc
     7981 atgatcggct ggcggtcata caggaaatcg tcctgattct gtgcccgttc cgtgtcggac
     8041 agacctgcgt gataccgcgt ggctggaaag ccgttttccc gcagagcatc acagacttcc
     8101 tccaccgctt ttcgggtgga gcagtacaca atgccgcttt tgccgtcgtt ttcccgcagc
     8161 agctgaaaca gttcccgcgt cttattattg ggacggcgta ccccgaaaaa caggtttttc
     8221 cggtcgaatc cggtatgtat gatttccggc tcctgcaatt tcagaatccg tgcaatatcc
     8281 gcctctacct gctttgttgc cgtagcggta aaggcggtac agatcggacg tgccggcagc
     8341 tgttccataa acaccggaat atccagataa ctctgccgga aatcctgtcc ccactgggac
     8401 acgcagtgtg cctcatcaac ggcgatcatg ctcaccggca cctgctgtgt cagacggaaa
     8461 aagctgtccg tcatcagccg ttccggtgca acgtacagaa tccggcactg cccctgctgt
     8521 accatttgca ccgtgtgata gaattcttcc tgcgtaatgc tgctgttcac atacgccgca
     8581 gggataccca cctgccggag agcctccacc tgatctttca tcagcgagat cagcggagaa
     8641 atgacaatgg tcaccccgcg gagcagcagg gcagagatct gatagcagac cgacttgccg
     8701 gctccggtag gcatgaccgc cagcacgtcc cgtccggaca agatcgcatc gattgcctgc
     8761 ttctgtccgg tgcggaatga cgcatgccca aaatattgct gtaacagctt ttccggctgg
     8821 ttcatgtttc ctgaccgtcc tctccctctg ccagcagatc tttctgccag cgtttcaatt
     8881 cttcccgtgc agtttccagc agcagcacgc tgggattttc accgcccatg atgcgggcga
     8941 tctcatggac acgtccctcc ggttccagta ccatgacatg ggtttccgtc cgcgtgccct
     9001 cggtctgctt ctcgatcatg aggtgctgat ctgccattac ggcgatctgg gacagatgcg
     9061 tcacacagag cacctgccgc agctgtccca cttcccgcag cttcatgccg attttctggg
     9121 cagccttgcc gctgacaccg gtgtcgatct cgtcgaagat cagcgtggga atgctgtccc
     9181 gatcggcaat gacacacttc agagccagca tgatgcggga cagttcaccg ccggaagcga
     9241 ttctcgccag cggacggggt tcttccccgc ggttggcaga aatcagaaat tccagactgt
     9301 ccatgccgtg aatggtcagc ttgccggtgg tatgtttccc caccaggatc acgttgggca
     9361 tattcagaaa tgccagctgt tccgtcacgg cagtgataaa gcgttccagc gtctgtgcac
     9421 ggtattccga cagagccttc gcctgtgcgg aaaccgtttc cagcagttgt gccttctgct
     9481 cccgcagggc agcgatctct gctgtgtcgc cctgcatctg tgccagttct tcctctgcac
     9541 ggtcacggcg ttccaccagt tcgtcaccgg tacactggta ctcccgactt agccgccgga
     9601 tctccagcat acgcttctgc accatcgcat accgctgggg gtctaagtcc atgccgtccc
     9661 gtagactgcg gcactccgaa gcaatgtctg ccagttcaat gcctgccgcc gccagccgtt
     9721 ctcccagcac ggcaaactcc gcccgcatgt cggaaaacgg cgacagttct tctgccgccg
     9781 cctctgtcag cagtactgcg gaaccgtcgg catcttcgcc gtcgattgcc tccgctgccg
     9841 cctgcaacgc cgcggcaatg ctctcggcgt gctgcagcag gttcagttct tcttccagtg
     9901 cctcttcttc gccctgttcc agtcccagtt cgtccacttc tgccaccagt gctttcagct
     9961 gctgccggcg gtgtgccttg tcctgttcct gttttgccag atgattcagc cgcttcgccg
    10021 tctgctgtaa ctgccggaat gtgtcctgat actggtgcag caatgtgtca tctccgccga
    10081 aagcgtccag aatctgcaag tggcactccg gtgccagcag cacctgattg tcgtgctgcc
    10141 cgtgaatgtt gatgagagcg tccccgatct cccgcagcac cgaaactgtt gtggtatgtc
    10201 cgttcacccg tgctgcactg ccgccgtctg cggcgatctc tctggtgagc agcaattcat
    10261 cgtcggtgtg ggaaatggaa agtgcgtcca gtctggcaca gaccagcgga tccagatgcg
    10321 tgaaaagtgc cgtcaccact gcctttttgc agcctgtccg cacaatgtcc cgcgtgacac
    10381 gctgtcccag aaccgcatgg atcccgtgca gcagaatcga ttttccggca ccggtttcac
    10441 cggtaaacac attcagatgc ggtgaaaagg gaatccgtgc atgggcgatc accgccagat
    10501 tctcgatctc cagttcagct agcattaaaa ccctcctgtt tccatcagct ttgcgaagct
    10561 gccggcaaat tcttttgcct gtatttctgt ccgcagcaat acaaaaatgg tatcatctcc
    10621 ggcaatggtt cccaccacat cttcccgatg catggcatct aacgccgccg ccactgcctg
    10681 tgccgtgccg gtacggcagt ggatcactgc cacatttccg gcaaagtcgg tacgcagggc
    10741 agcgttctgc aaaatgccgc cgttgacttc ctgccgcacc ggtgccgtat agcatgccct
    10801 gccgctgcca cgccgttcct tggtcagctt cagttcccga atatctctgg aaagcgttgc
    10861 ctgcgtcacc gcaaacccgt gttcctccat gcgttcccgc agcacttcct gcgtgggaat
    10921 ctcctctgct gcaatgagcg aaagaatggt atcctgccgc tttttcttca tgtgttcctc
    10981 acgtccttcc cgctttcaaa gactgcatca gcttccggct cagcgaatca aaaaaggtat
    11041 tgccgctaag gtccagaatt tccagcgtct ggtcagaacc ggaaatttcc atgactcgtc
    11101 cctccggaaa cggctccggt ttttcgccgt ctgcacagaa ataggcatca tattcctccc
    11161 gcacccgcag gctcacctgc aaatcgttgt ggggagaaaa caggatcggt ctggcagaaa
    11221 gcgaatgggg acagatcagg ttcatctcaa tgcaggaaaa ttccggttcg ataatgggac
    11281 ctccggcgga gagggcgtag gcagtcgaac cggtaggcgt gctgaagatc acgccgtctg
    11341 cccgatattc gcccaacacc acgttgttgc gggaaacggt aaactcaatg acacgggaaa
    11401 agttgcggga gatcacgatg tcattcaacg ccgtcagttc ccgcaggcag gtgccggtct
    11461 tgtccagcac cttgccatgc agcagcatgc gtttggaaac ccgatagtcc cccgtttgca
    11521 gccgtgcaat ttcaccgatc tgatccggct ccatagatgc cagaaagccc agccgtccgg
    11581 tgttgatgcc cagaagcttt gcctttgtgc ccaccacgtg acgggcacag tgcagaatgg
    11641 taccgtcccc gccgatcgcc actgccacgt ctgcgtcctg cactgcttcc agaaacggag
    11701 cataacagat aaaggacata tcccccagca gtcccagaca gcggggatct gcccagactt
    11761 ccatgccgtt ccggtacagt gccagacaga cctttcgggc aacttccggt gcgttgcctt
    11821 tttgcagatg atgaaataac acgactttca tttcgtcttc tccttttttg cctgtgcgaa
    11881 tgcctgctgt accactgcgg caatttccgg cagatacgcc gcttcgccgc ctttttgtac
    11941 aacggcgaga tactcaatat tgccggagcc gccctgaatg ggcgaagggc acagcagctg
    12001 tacagtcagc ccctgtgcgg aaaacagctg caagatgttg ttcagcacct gcatgtgcac
    12061 gcgggaggac tgcaccagtc cgtttttccc gatgttttcc cgtcccgctt caaactgggg
    12121 cttgatgagc accacgccct gtgcccctgt ccgcagcagc ggcagcacgt agggcagtac
    12181 atggtgcagc gagatgaaag acacgtccgc cgagaagaaa tccgccggct gttccagctg
    12241 ttccggtgca atgctgcgaa cgtctgttcc ctcccaattt atcacgcggg gatcctcccg
    12301 tagggactgc accagctgtc cgtgtcccac gtcaatggcg tacacctttg cagctccgtg
    12361 ctggagcatg cagtcggtaa aaccgccggt agatgctccg atgtccagac agaccattcc
    12421 ctccagagag agccgcatct gctgtaccgc atgttccagt ttcagaccgc cccgtcccac
    12481 aaaggggatg tcactgccct ccatggcgat ccggtcggtt tccgaaacgg caaacgccgg
    12541 cttggtacag accttgccgt tcacagagat ctgcccgttc tgaatcagcg ttttggcacg
    12601 ctggcggctg ggcaccatac cctgctgtac cattaccgca tcaagccttg ccatgttttg
    12661 cctcctgctg catatcctga taaatggcat gctccgacag ccccatagtt tccatttgag
    12721 aatccgtaga agcctgcggc agatatgcct tgggtgctac ccgccggtaa gtgccgcgga
    12781 agccgcgttc cagcagcaga tctcccagca gctgggaaat tccgccgtaa tagtatgcct
    12841 cctccagaaa taccacatgg gcataggaca tggcaatttc caccacgccc tctgccagcg
    12901 ggaaaatgcg tgtcagcttc agcaggtcac agaacacgcc ttcctccgcc atgcggcggc
    12961 tggcacggta caggtgatcg tacaccctgc cgtaggaaat atacagggta tctgtttttc
    13021 tgccggaaac gtgggtgtat tcggtattca gggcagtctt gtcaaaggaa accttttcgc
    13081 tgcctctggg ataccggata catgccagcc ccttatcccg atagagtgcc cgccgcagac
    13141 agagccgcat ctcctcgtag catgccggtg cataaattac cgtattgggc accgtgcaga
    13201 ggaacgacac atcgtacagt ccgtggtggg tttcgccgtc ctcgccgaca acgccggcac
    13261 ggtcgatccc cagcaccaca tgcatgccgc caatggcaac atcgtgcatc agctggtcat
    13321 aggctctctg caaaaaggaa gaatacaccg caaataccgg cagttctccc atagaagcca
    13381 gtgccgcgga aaacgtcacg gcgtgctgtt cggcaatgcc cacatcatag taccggttcg
    13441 ggaactcccg tgcaaaatgg tgcagtcctg tgcccccgcc cattgctgcc gttacggcac
    13501 aaatgcgggg gtcgtgtttc gccagctgca ccagttcttt gccaaacatc tccgaatagg
    13561 aatccgcagc ggaaacctcc ggattgccgg tttcaatgtc aaacttggag attccgtgat
    13621 actccgacgg ctttgcctct gccggtgcgt agcccttgcc cttgacggta tgtacatgga
    13681 taaacaccgg acggtgatat tcctttgccg ccagcagcac ttcttccagt tccaccagat
    13741 tatggccgtc caccggtccc agatagacga aaccgaagtc ctcgaagatc gttgccgact
    13801 gcaacaatac gtcccgtaca acgctcttgg aattcttgat gactttcgcc acagacggac
    13861 cgatcaccgc ggtgcggttc aggcggcgtt ccaccgcctt tttggtgcgg acgtatcctt
    13921 cactgctccg catggaggac aggtagcgtg ccagtgcccc cacgtttttg gaaatggaca
    13981 tctcattgtc attgaggatc acaatgagat tattcctcga ttttccggca ttgttcaatc
    14041 cttcatagga cagcccgccg gtcatggctc cgtcccctac aacggcaacg gcaaaatgct
    14101 ccttgtcccc gtgcagccgc atgccctctg caatgccgca tgccacggag atcgcagtgc
    14161 tgctgtgccc gctgatgaag ctgtcgtgtt ctgattcgtt tggcttcggg aaaccggaga
    14221 tgccgttttc ctgccgcagg gtcgaaaatc gttccagccg tccggtcaaa attttatggg
    14281 tatagcactg atgccccacg tcccagacga atttatcttc cggcgaatca aacacacggt
    14341 gcatcgccac cgtcagttcc accgacccca gattggaagc gagatgcccg ccggttttgg
    14401 aaacagtttc tatgagaaga cttcgaatct ccgcacagag tgcggtacac tgttccggcg
    14461 aaagtgcctt cagatcgtcc ggcgagtgca gacgctccaa caggtgctcc tctgtttccg
    14521 aaattttcat atattccatg gttttgtttt ctgcggctcc tttgccggcg gcacattccg
    14581 cggcagttcc gcaggattca ctccttcgat tatttttcac ggcacagcag ttcccgtgtc
    14641 agatccagca ggaattcatg ctgcgggaac tgggacagga tctgttctgc ctctgccgtc
    14701 agtgctgctg cacgtgtctt ggcagcttcc agccccaaca gagtgacaaa cgtggtcttg
    14761 ccctctgcgg catcgctgcc caccggcttg cccagcactt cagaggtact ggtcacgtcc
    14821 agaatatcgt ccacgatctg gaatgccagc cccagctttg ccccatactg atctgccagt
    14881 gcgacctgtt ctttcggagc aaatcccgct gtacagccca gccggcatgc tccgcgaatc
    14941 agggcaccgg tcttggcggc acacatggct tcctgttctt ccagtgtgac atcgctgcgt
    15001 ttctcgtttg ccatatccat gatctgaccg ccgaccatac cggctgcacc ggttgccgaa
    15061 gtaaggattt gcagcagttc cactttcatt tccggttcca gtatcggatc acgcagaatt
    15121 acggcaaatg cttctgtcag cagggcatca cctgcctgca acgccacagc atgtccgaat
    15181 gccttatgac aggacggctt gccgcggcgg taatcgtcgt tgtccatttc cggcaagtca
    15241 tcatggatca gggaaaaggt gtgcaccatt tcaatggcac acgccgccgc cattgcctgc
    15301 tcctccgagc cgccaaaaat ccggcagaat tccagcacca gcaccggacg gatccgcttg
    15361 ccgccggctt ccagagaata ccgcatggct tctaccacct gtgcctgccg ccgggtttcc
    15421 ggtgtcacct gcacatattc caacagtttt gcatctacct ttgccgccag ttcttccaga
    15481 tacgccttat gcttcgcttc catcatgaac ctcctgttcc tgtgccggca ccgtctgttt
    15541 ggaaatttgc agctttgctg tctgcaattc ttcctgacag gataccgcca gttccatgcc
    15601 cttgtgatac agctgcaccg cttcttccag cggcaggttg ccctgttcca gcttctgtgt
    15661 cagttcccgc aattccttca tgtgtgtttc aaatgccata tgcctgctct ccttccccaa
    15721 aaacacgaaa atacgtggtg ttgtcttatc tttctggcaa taccgcacaa agattgtgcg
    15781 acagatgcgt cgtcaaatt
//
LOCUS       DAWBGR010000040        15705 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1226_length_15705_cov_3.591246, whole genome shotgun sequence.
ACCESSION   DAWBGR010000040 DAWBGR010000000
VERSION     DAWBGR010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15705)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15705)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15705
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1226_length_15705_cov_3.591246"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    13915..13924
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4076 a         3776 c         3764 g         4079 t
ORIGIN      
        1 caaccaatct ttccacatat acaaagtaat tttgccataa cacacctcac ttacaaattc
       61 cgatttgcca agcaaacagt aatccctact tcttcaccac attcaatttt gcaatctcca
      121 tatcctgcgg cccggtcaga gaacagccct tttccttggc gtactggata taattcacaa
      181 tatccgccgt aaagcgttcg ccgggggcga gaatcgggat tcccggcagt gcctgttctt
      241 ttgtcatgtt gattctcctt tgcaaaccag aagttgtaag tgatttaaag gaaaatttgg
      301 ttttaatcaa tgcggtaaac ccgggattta ttcttcaaaa ctttttgggt ctaacagata
      361 agctatatca tcttgaaatt ttagtggtat tctgtttctc ttttcttcga taagtggaaa
      421 tggtggtaac tcatagctca tcagtaattc tattgcatct tctattacat catcttcatt
      481 tggttcagta atcactgttt gtaacaataa cactaaatca tcatgaatat cacttatatc
      541 ctgctgataa tatgggtcca taaaccaatc taaacaaaaa agcatactga gttttctttg
      601 ggtatcatct ccaagtaata cctttgcaat ttcacatatt ccttttctga caatatctct
      661 atctttgtct gtatatgtaa caaatctgcc attctccata atttatactc cccgattgaa
      721 attactggat tatatttttt taattattca aattcttttt tactcactta agtcccaatt
      781 taaaccagtt cgtcaaattc cgatttgcca agcaaacagt aacccctact tcttcaccac
      841 attcaatctt gcaatctcca tatcctgcgg accagtcaga gaacaaccct tttccttggc
      901 gtactggata taattcacaa tatccgccgt aatgcgttcg ccgggggcga gaatcgggat
      961 tcccggcgga tagcacatga caaactcact gcaaatttca cctacgctct gttccagcgg
     1021 cacagccttt ttgtccgcat aaaacgcctg ctgcgggggg agtgccacgt ccggagccag
     1081 atactcgctt tgcagcatgc ccgtaggatc gccgccgtac agccgcttga tctccgacaa
     1141 cgccgagatc agccgttcga tctccaacgc ccgatctccg gcagtgacaa tggcaaggat
     1201 attgccgatg tcgccgaact cgatctgaat gccgtattcg tcccggagca agtcgtacac
     1261 ctcaatgcct gctagaccgg tgtttcgggt gtgaatggac aattttgtcc ggtcaaacgc
     1321 atagaacgaa tcgccgttgc aaagttcgtc cccgtagaca taatacccgc cgatgcggtt
     1381 gatttcctcc cgtgcatagt ccacatattg caccgctttc cggtacattt cccgtccgtt
     1441 catcgctaga tttcgccgtg caatatccag cgataccatg agcagataag acgcactcgt
     1501 ggtctgtgtc aggttgatga cctgccgtac atagtccgca ttgatgccct ctgcacagag
     1561 cagcatggca ctctgtgtca gactgccgcc ggtcttgtga atgctgaccg atgccatatc
     1621 tgcaccggca gccatagcgg aacaggggag attctccccg aaatagaaat gtgtgccatg
     1681 tgcttcgtct gccagcagca gcatgccgtg ttcatgggca agcttggcga tacggcgaat
     1741 atccggacag acaccgtagt aagtcggatt gtttaccaat acagcttttg cgtccggatt
     1801 ggcaagaatg gcacgttcca ccgcttctac tgacatgccc agcggaattc ccagatcctt
     1861 gttttcgtcc ggatccacat aaacgggaat agccccgttg acaaccagtg cattgatcgc
     1921 actgcggtgc acattgcggg gcatgatcag cttttcaccg gctttgcaga ccgacataat
     1981 catagcctgc gaagcaccgg tagcaccgtt gatgataaaa aaggcattgg cagccccgaa
     2041 cgcttccgcg gcaagctgct gtgcatcgcg gagcacggaa accggatgac agagattgtc
     2101 cagatttttc atagaattta cgtcgatctg tacgcaccgc tgtccgagaa aatccgccag
     2161 ttcatggttg ccccgtccgc ccttgtgtcc gggaacgtca aaccgtacta cacggttcat
     2221 cagatgctct ttcagagcgt ccatcagagg agcgtcctgc tgtgtccagt tcatatcgct
     2281 ctgcctcctc aatagatgtt tctgccgctg aaaatttcga tcatttcccg ccgcagacga
     2341 ttggtaatat ccagccgcac ccgcgggtcc agttcatatg catcggtttt gaacagataa
     2401 ttttgcagtt ccagttcctt gatcagcatg cgggtgtgaa aaatattgga ttgatatacg
     2461 tttacatcct gtgcatcata ccgcaggaga atttcttcgc tgataaaatc ctggatcgaa
     2521 gccatttcct gatccataaa gcactttttg ccggaaacat ctctggtaaa gccgcggacg
     2581 cgataatcaa tggtgataat atccgaatcg aaactccgga tcagataatc cagcgtgttc
     2641 agcggagaga tttccccaca ggtggaaaca tcaatatcga cccgaaatgt ggtgacttca
     2701 ttgtccggat gactttccgg aaaggtgtgc actgtgacat ggctcttgtc cagatgtgcg
     2761 tgaatatccc gacgttggag aaatccgtcc ccctgattgc aggacggatc gatgctttcc
     2821 ggtgctacac tgccttcagc gatcaataca ttgaccgaag ctccctgcgg ttcgtaatcc
     2881 tgtctggaaa tattcagcac cgccgcaccg atcatttcgg tgacatcaca gagaatattc
     2941 gttagacgtt cggaattata ctgttcatcg atgtagcgga tatagtcccg tttttcccgt
     3001 tcggactttg cataacatac atcaaaaata ttaaagctca acgtcttagt gagattgtta
     3061 aatccatata aggtcagctt tttctccaac cctaccatca cagcctttct gaaagcatct
     3121 gcccgcagac agatgccctc taaaaatcga aaagtagaag tatgggagca gcagtcctgc
     3181 tcctgtctgc ggtaaaagat tgcaacaccg cagttcacgt tcttacaatc aaaaaccatt
     3241 gtaccacagt ttgcaaaaaa atgcaatggg atttacaaaa cttttttcga aaacgacggg
     3301 gagaaacaac atttccccgc cggagaagat tccaattata gtttctcttt ttccaatttt
     3361 tgaattttca ggacttacca cttgcaaaat atcacgaagt atagtataat agaacaaatc
     3421 gaatgaaaca gtgacgaaat gccgcatggc agggggagca gaaaatagga gttactgcga
     3481 tacgtttcaa ggcaattctg tacaagcttg tgcagtactg ccgcatggaa aacgcacagc
     3541 caagccaatg gattgtgaga tttccggatt cgcagaggca tggggagaac ttgccgctgc
     3601 acgaaatgct gatgcagaaa ggaagacaga aatcatgact gaaaccattg aaaatacaga
     3661 aaactatatg gaagaaatca ccgcaccaac cgtacaaaaa ctgccgctgc ttgcaatgcg
     3721 tggaattgta ttgtttccga acatgatcat gcacttcgat ctggctcggg aaccgtttgt
     3781 caaggcattg agagccagtg ccaaatcgga tcgccgcgtg tttctggtga ctcagaaaga
     3841 ccccttggtg gaagaaccca aacaagacga tctgtataca gtgggtgtga tcgcagaagt
     3901 gcggcaggtg ctccgttcgc cggacggcgt gacacgggta ctggtcgagg gaaaagaacg
     3961 ggcagccatt accgcagctt ttctggaaaa cacagaaaaa gaaaaggact tttacccgca
     4021 ggcagaagtg gaactgctgc cggaagtttc ggcagaggaa gaccgtgaaa aggaactgac
     4081 cgcttctgtc cgctgcataa aggacatttt tcaggaatat gcagaactga tgcctcgcat
     4141 gccgaaagaa ttgatggcaa ctgtgatctg cgaacaggac gctgccaaga tgtttaacga
     4201 gattgtgttc aacatttcgc tggactatga tgcaaagcag atgcttctgg aggaaagcag
     4261 cctgctgcag cggctggaaa agctgtaccg gattctggcg gacgaaatgg acattctccg
     4321 gctggagcga aaactacagg aggaaacaca agaaaatctg gatcgctcgc agcgggaata
     4381 ctacctgcgg gaacagatgc acctgattgc tgaacagctg ggagaatccg aagatccggc
     4441 tgcggaaatg gaacagtata ccgacatcat cgacacgctt ccgctggacg aagccagccg
     4501 caaaaagctg gaaaaggaag cagaacgtct gtcccgtctg ccggcagctt cacaggaagc
     4561 ctttgtcatt cgcagctatc tggacacagt tctggcactg ccgtggaata aatccagcca
     4621 taccaagccc aatctgaagc gggcacagtc tgtactggaa aaggatcatt atggtttgaa
     4681 aaaggtgaaa gaacgaattc tggaaagcat tgccgttcgg acactgctgc cggaagcctc
     4741 cggacaaatt ctgtgttttg tcggacctcc cggcgtgggc aaaacttcta tcggcagatc
     4801 gattgccaag gcactgggac ggcactatga acgtgtttct ctcggcggtg tgcgggacga
     4861 atcggacatt cgcggacacc ggaaaactta cgtaggggca atgcccggac gcatcatgga
     4921 tgccatgacc cgtgccggca ccaacaaccc catgatcctg ctggacgaag tggacaagat
     4981 gagcaacgac tttcggggcg atccctctgc ggcactgctg gaagtactgg acagcgaaca
     5041 gaacaaggcg ttccgtgacc actatattga agtgcctttt gacttgagca aggtgctttt
     5101 tgtggcaaca gcaaactctc tggatccgat tccggcaccg ctgctggatc gtatggaaat
     5161 tatcgaactg ggcagctata cccgtgagga aaaattccac attgccaaag agcatctgct
     5221 gaaaaagcag ctgaaaaaac acggactgaa aggcacccag tgccggattc cggacgaggt
     5281 gctgtatgcg atcattgatg gctataccag agaagccggc gtgcgggaac tggaacgcac
     5341 gatcggtacg ctttgccgaa aggctgccaa agaaattgtg gagggcatct gcaaaaaggt
     5401 ttcctttaca gaagtcaacc tgaaagaata tctgggaatc ccgaaattcc gaccggacga
     5461 acagagccat gaagatactg tcggcgtagt caacggtctg gcatggactt ctgtcggcgg
     5521 cgtgctgatg cctatggaag tgctggtgat gtccggcaag ggaacagttg agtacactgg
     5581 ttcactgggt aacgtaatgc aggaaagtgc caaaattgcg gtcagctatg cacgcagcgt
     5641 ggcaaagcag tacggcattc cggaagattt ccacaccaag tgtgacctgc atattcatgc
     5701 accggaggga gctgttccca aggacggtcc ctcggcaggc gttaccatgg caaccgcgat
     5761 catttccgct ctttccggca gaccggtgcg gggggacatc gcgatgacgg gggaaatcac
     5821 cctgcacggc aaggtgctgc ccatcggcgg actgcgggaa aaatctatgg cagcttataa
     5881 agccggcatt cacacggtga ttattccgga agaaaacctg ccggatctgg aggaagtaga
     5941 cgaaaccgtg cgggaacatg tcacatttgt gccggcaaaa acgctggaca ctgtgctgaa
     6001 tacggcactg gtaccggcag aaccagccgc agaagcacag acagccgctg cggaactgtg
     6061 ccatgtataa aaaggagaag aatccatgca gtttcaaaaa gcagaattcg tagcgtccta
     6121 cgggacattc tcacagatac cgccctgcaa ttccatggag attgcctttg caggacggtc
     6181 gaacgtggga aaatctacgt tgatcaataa aatttttcag cggaaatcgc tggcgagagt
     6241 cagtgctgtt ccggggaaaa cagcgacaat caacttttac cgtctggatc ctttgacgat
     6301 cgttgacctt cccggctatg gctatgctaa ggttgccaag tcggaaaagg aacggtggtc
     6361 cggactgatc tcgggctatc tcggtgcaga tcgggacttg cgtctgatcc tgttactgat
     6421 cgatatgcgt cacgcaccct ctaaagacga tttgcagatg atcgatttct tggtagaatc
     6481 ggaactgccg tttttgattg tgctgaccaa agcagacaaa ctgaacaaga cagaacgtac
     6541 caaacgcatg caggcatttg aaacagaaat tccgtacttc tcacagattc atgtgatccc
     6601 tttttccgca gtgacgcggg agggtgttgc agaagtacag ggcgttctgg aagaaatcgc
     6661 ctccgaaacc gaagaatgat ctgcggggtg aacgggagta ttttcaccgc gataccgcgt
     6721 tcataaaagc aatgcagaaa ggaagtataa acatgtttgt atctgataat ctgggggtaa
     6781 atgcgtccgg aaatctgacc attggcggcg tagacaccgt tgcactggca aagaagtatg
     6841 gtacaccgct gtatgtcatg gacgaggaca tgattcgtcg ggactgcaaa acattccaga
     6901 aaagcatcga cacatactat gacggacagg gtctggtctg ctatgccagc aaggcatttt
     6961 cctgcaagga gatctatcgc gtgatgcagt ccgaaaatat gggcatcgat gtggtttccg
     7021 gcggggaact gtacaccgca gtgcaggcag gcttcgacgt gacaaaggct tgcttccacg
     7081 gaaacaacaa gaccacagaa gaactgaaca tggcactgga ttacggcgtt ggcagaattg
     7141 ttgtggacaa catctatgaa atggagcacc tgaaccagct ggcagcggaa aagcatctga
     7201 ctgccccgat cctgctccgc atcaagccgg gcgtagatgc acacacccat aactttatcc
     7261 gtaccggaca gattgacagc aagttcggct ttgcattgga aaccggcgaa gcatacgaag
     7321 cagtcaagaa agcgatttct tacccgaatc tgaagcttcg cggtctgcac tgtcatatcg
     7381 gctcgcagat tctggataca cagggcttta cggcagcagc agaagtcatg atggaattca
     7441 ttgaaaagct gaagaaagaa ctgggctacg aagcagaaga tctgaatctg ggcggcggtt
     7501 tcggtatccg ctatactgaa gcagacgacc cgctgccgta tgatgcatat atggaagcgg
     7561 tagcaaaggt catcaacggt ttctgtgcat cgcacgatgt aaagcgtccg tttatcctga
     7621 tcgaaccagg ccgttccatt gcggctccgg caggcattac cctgtacacc gtaggcggca
     7681 tcaaagagat tccgaatatc cgtacttatg tttctgtcga tggcggcatg tgcgacaacc
     7741 cgcgttatat cctgtatcaa tctgcatatg atgcactggt tgccaataag gcaggacagc
     7801 cgaaaactga aaaggtaact attgccggaa agtgctgcga aagcggcgac ctgatcggcg
     7861 aaaatatgga gattcagcct tgcgaagtag gcgatattat tgcggtttgt gcaactggtg
     7921 cctataatta ttccatggca tcgaactata accgactcca gaagccggca gtcgtttttg
     7981 taaagggcgg cgaagacaga ctggcagtga agcgggaatc tctggacgac attctgcgga
     8041 acgacatcta agacaatcaa acgttaacat aaggcaatac cgcacaaaga gcgtctggcg
     8101 actttgtacg gtattgctat aaaataacag caaacaaaac ggcggcgatc cgaagaaaac
     8161 ggattgccgc tgttttgcaa tttggttaca ctataaagag cacttttcag aatgggaaat
     8221 agcttatcgg caagcttttg cagcttctac catgttttcc aagctgggta ctgtttcagc
     8281 aatacctctg gtttttagac cgcagtccgg attgacccaa agcttttctt tcggaatacg
     8341 ggagagcatt ttggaaattg ccgttttgat ttccgcagtg gtagggattc tgggggagtg
     8401 aatgtcatac acgccggggc caacttcggt gcggaagtgg ttctcctgca atacgtccag
     8461 aatcgtcaga tcagaacggc tggcttcgaa cgtgatgaca tccgcgtcca tatcatcgat
     8521 gtcctgaatg atgtcagcga attcgctgta gcacatatgg gtatgcacct gtgtttccgg
     8581 ttttacaccg ctgtgcacca gtcggaatgc cggaattgcc cagttcaggt attctgtttt
     8641 ccagtcagat tttcgcaaag gcagcttttc ccgcagagca gcttcgtcta cctgaataat
     8701 gcggataccg tttttctcca gatccagtac ttcttcccga atggcaagtc cgatctgata
     8761 cgcacaatcc tgcaaggaaa tgtcttcccg cgggaatgac cagttcagaa tagtgaccgg
     8821 tccggttagc atgcccttca tcggcttttt tgtcaggctc tgggcataga cagaccattt
     8881 tacagtcatc ggctttgcac ggctgacatc accccagacg atcggcggct ttacacagcg
     8941 tgtgccgtag gactgtaccc aagccttttc agtgaacagg tagccttcca gacactctcc
     9001 gaaatattct accatgtcgt ttcgttcgaa ttccccgtgt accagcacgt ccagaccgat
     9061 tttttcctgc aatgccacgc agtctgcgat ctgcttcttg ttgaaatcta catattgttc
     9121 ctcggagatc tcgccctttc ggaatgcagc acgattggca cggacttcct ttgtctgcgg
     9181 gaatgaaccg atagttgtgg taggcagcag cggcagctgg aatgtttcct gctgaatctt
     9241 ttctcgttct gcaaaagtcg gctttcgaac aaaatcagct tctgtcaagg ctgcaacttt
     9301 tcgttgtact tctgcattgc tgcaatgtct gtcctcgcag aacaactgct gatttttctg
     9361 atatgcctct gtcgcctgcg gctgtgcaga agcacagatc tgtgacagtt ctgccagttc
     9421 ttccagcttt tcttctgcaa aagcgaagtg cttctgatag ctctccgcca gcttggtttc
     9481 gtttcgcagc gtgtacggaa cgtgcagcag agagcaagag gtgttcagta cgatctcacc
     9541 gcaaaccgga cgcagctgtt ccagaatttt cagggttttc tcataggaat tccgccagat
     9601 attttttccg tttactacac cggcaaacag aattttttcc ttggggaatc catttttctg
     9661 taccagtgcc atcgtctgtt ttccctctgt gaagtccaga ccaataccgt caaagtccag
     9721 tgctgtgatc tcagaatagc agtcccgcac atcgccgaag tatgtttgca gcaacacctt
     9781 gacattgccc tttgccgcca agacagtctt gtaaatctgg gtaaacagag caatatcctc
     9841 tgcggacaga tcttttacca gatacggttc gtccagttca gcccatgcag cacccagttc
     9901 attcagcttt gccagataat cgcagtatgc cttgactgta tcttctacaa agtcagagag
     9961 tgtcttttca ccagtgaatc gtgccagctt caggaatgta aacggtccga tcagcaccag
    10021 ctttgtcgga acgcccagtg cctttgcttc tgcatattct gcaaaaggct tttcgccggc
    10081 aaggcgaatt tctgtggtgt cctcaatttc cggcaccata tagtggtagt tggtgttgaa
    10141 ccactttttc attgcgaatg cttttacgtc accgtgttcg ccctgatagc cacgtgccat
    10201 tgcaaaatag gtgtccagtg gcgacagaga aagagcacga tagcgtttcg gaaccgcatt
    10261 cagcagaacg gcggtgtcca gcatgttgtc atagaaagaa aagtcgttga caggaatcag
    10321 agagattcca ttttccttct gcagctgcca atgggtgcac cgcaattctt ttgcggtgtt
    10381 ctgcaattct tctgcggaaa tttctccgcg aaaatacttt tcgctggcaa atttcagttc
    10441 ccgcagactg cccactctgg ggaatccgat aatagttgtt ttcataagat aactccgttt
    10501 ctggggaaac acatgcttgg gcaaccatgt aaactgaggc aataccgcac aaagagcatc
    10561 tggcgaaaaa tttgacgacg catctgtcgc acaatctttg tgcggtattg ccctgaaaca
    10621 atgttgccca gcgtattccg ttttgtttct gattgtaatt ccattataca ggattccacc
    10681 ggacatgtaa aatatataaa aataccggtt tgctatagct ttattatata gcaaaccggt
    10741 atttgaggtc tgtgctgcat cagagcctat tcagtatttt aggacaacac cgcacaaagc
    10801 ccgcctgacg gctttgtgcg gtgttgcctt aggagttttt cgcataacgc tttaatgcat
    10861 ctaaataaat gctacccaat cggctgggca ctgcatgctt tttggtgatg taaccgatat
    10921 gcatgcttcc gttttccgca agaggaattg ccacaatgtt ttctccgttt agttcttcgt
    10981 caatgacacc gctgcaaatg gtatacccat ttagtccgat caacagattg aacagcgttg
    11041 ccctgtcccg tacccgaata tttttcgagc gaatttctgt gctgaaaatt tcttcagagt
    11101 agtaaaacga gttgtgttcg ccctgttcaa aagacaggta cggatagggc tgcaagtctg
    11161 ccatggtgac ttctgctttt tgtgcaagag gatttttgtc gctgataaaa atatgcggct
    11221 tcgcttcaaa caattccgta aactgcaagt cgttggattt caaaatcttt cgcaaaacgg
    11281 tttcgttgaa ctcgttgaga taaagaattc cgatttcact tttcagacgg gaaacgtcat
    11341 caatgatctc ataggtctgg gtttcccgca gactgaaatc ataattgtca ctgccgtaca
    11401 cctgaatcaa gtctacaaat gcatttaccg caaaggaata atgctgtgtc gaaacgcaga
    11461 attcctgttt tccaccggac tggtgggtat acttttccgt gatcagagca gcctgctcca
    11521 gaacttgtct ggcatagccc agaaacactt ccccctcttt ggaaacaaaa acgcctttat
    11581 tggtgcggtt gaaaatggag atgcccattt ccttttccag ttccttgagt gcttttgtca
    11641 ggctgggctg ggcaatatac agagaagcag ctgcttcact gactgttcct ttttccgcag
    11701 tgacaacggc atatttcaat tgctgtaatg tcatacggct gtttcatcgc tttccggctc
    11761 tgcttccggc aagtctgtga gatccgattc cggttctgtc ggctccggct gtaattctgc
    11821 aatgcgagcc gtcaacccgt tgatttccac agccggatcg tccagatcga tcagcagata
    11881 ccgtctgccg tctacgcact gtgtccgcac cttgtctgtg gcatctttcc caatcgaaac
    11941 agtgatgccg tctacgctga tcgttgtttt gttgtcgatc aggttgttga cagtaatcgg
    12001 cttgtcctgt gttgcctccc gatatgccat ctgcatttct tctgcacgtt cctgactaac
    12061 gccggagtcc agcaaaatgt cccgcacgtg aatatcgtcc acaatgggca gatccggttc
    12121 attggcgtat tctgctgcga tatcccgcag cttttcattt acgccggtga tgacagagta
    12181 actcagttca tcggaaacga tttcctgcat catgctctgg aatgtttcct tctgttcctg
    12241 agccgtccgg ttaaacgggc agcccagtac attttctatg atgctgatat taacatcttt
    12301 gggcttgtgg gcggagtaca gcacatggtt tacgtccgga cctctccccg tgaaagttgg
    12361 atagacaaac ccgtctgtgg ggatctctgc cacgattctg tcgtaaacat ttttcttttc
    12421 aatggcattg tcatcttcgt tgtagatcag cccgtctacg cgaacttcta ccggacaaat
    12481 tgctgccacc agaaaacggt aatcgtggct ctgatacggg tctaattcgt cactcttgga
    12541 cttttgaaaa acggtataag tacaatgtcc gaccaaaatc gcataggtgg aaacatagtc
    12601 catgttgcgg acgatctgtt ccatcagcgt tgtaacggca gtttcatctt ccagtttgga
    12661 ctgtaacgct tcgtagagaa tcgcctgact gccgccttct tcataggctt ctttgggaaa
    12721 ttcgtattcc agcagtcctt ttcccagtgt gcccgcaaga acacgtttca gcgttgccat
    12781 aaggcagtcg ctttcttcgg aagcaatgtt gtgataggca cggctggtct gacagcggat
    12841 atttttttcg ctgtccacaa aagcggttgc cacctgattc agtacaaata aatcactggt
    12901 atctgaaaaa ttttttcgaa tttcggagag ctctttttta ttcatggaaa ttcctttctg
    12961 ttatctatgg aaacgctgcg taaaaattgt ttcgctgttt agcgtgcaat ctgtctgtac
    13021 gattttacag cacatcgttt cctttgcaaa agcattgcaa tgcccgtgca gtttctgcca
    13081 ccggcaagcc cattacgctg taaaaatcgc cgttgatgtt ctgcaccagc agtgcaccgt
    13141 atccctgaat gccgtaactg ccggctttgt ccataggttc gccggtcgca agatacgcct
    13201 gtatctgttc ttcagtcagc ggataaaatg ttaccgctgt ttctgtctgg aatgaggata
    13261 cgcgttctcc ttgtgccaaa gctacaccag tgatgacagt atgtgtcttt ccggagagtt
    13321 gacgaagcat ctctgccgca tgttccggcg ttttcggttt gccgaaaatg gcattttcca
    13381 acaccaccgt ggtatcagaa ccaagaacaa tgtcattcgg atattcgttg gcgacggcaa
    13441 gagccttttt tcgtgccaga tactgtgccg ctttctgtac agacaacacg ggcggcaggg
    13501 actcgtctac ggctgccgga aaaaccgtaa agtcttccgt gatcagccgc aggagttcct
    13561 gccgccgcgg tgatgcagaa gctagaatca gccccattac agcacgtcct tcgggtcgta
    13621 atgcggttct ttgtagatgt gttcactctc ggtgacttcc ttgcggaatt cgtcctgcca
    13681 ttctaccggt gtgaaatctt ctactgcaac agaaatgtgt tccggattgc agccgatcgc
    13741 atctaccagt gcagcggcaa ccttgtctgc agcaagcttt ttctgatcgt ctgttctgcc
    13801 tttgagcatt ttgatcgtaa tatggggcat ggtatgttcc tccttgaaat tctgtttctg
    13861 atatattgcc cgtgaaattt tcgggtgctt ttattatagc atattttata ggagnnnnnn
    13921 nnnnagtcgc cagacgctct ttgtgcggta ttgcctacag tatcattttt gcttttccgc
    13981 ttggttatta cggaatttag acagaaaaca ttgacaccac accctcaatg tgttataata
    14041 aaaaaagaaa tttacatgga catgacatac agcattgccg cagcgtccat gaatcataag
    14101 gagcaaaaac atgaaacaga ttattttgac cggagatcgt cccaccggac ggctgcatgt
    14161 aggacattac gtaggttccc tgaaagaacg ggtaaaactg caaaactccg gaaaattcga
    14221 cgagatttat attatgatcg ccgatgcaca ggcactgaca gacaatgcag agcatccgga
    14281 aaaagtacga cagaacatta cacaggtagc tctggactat ctggcatgcg gtctggatcc
    14341 ggaaaaaagc accattttca ttcagtccat ggtaccgcag ctgacagaac tgacgttcta
    14401 ttacatgaat ctggttaccg tttcccgcgt acagcggaac cctaccgtta aggcagagat
    14461 tcagcagcgg aactttgaag ccagtatccc agtaggcttt ttctgctacc cgatcagtca
    14521 ggcagcagac atcaccgcat tccatgcaac tgccgttccg gtcggagaag atcagctgcc
    14581 gatgctggaa cagtgcaagg aaatcgtgca caaattcaat tcggtatatg gcgaaactct
    14641 gaccgaaccg gagatcattc tccccagcaa tcaggcatgt ttacgtttgc cgggtattga
    14701 cggtaaggca aaaatgagta aatctctggg caactgtatc tacctgtctg atgagccgga
    14761 ggagatcaag aaaaagatca tgtccatgtt caccgatccg aatcacctgc gggtagaaga
    14821 tcccggtaag gtagagggta atccggtatt tatctatctg gatgcattct gcaagccgga
    14881 atatttcgag gaattctgga aagattacag ctgcctggac gaagtgaagg cacactatca
    14941 gcgtggcggt ctgggcgatg taaaggtcaa gaagttcctg aacaatgtaa tgcaggaaga
    15001 actccgtccc attcgggaac gtcgcaagca gtgggagcag cgtttgccgg aagtttacga
    15061 gatcttgcag cagggaagtg ctacagcgag agcaactgcg gaaaagactc tggaaggtgt
    15121 tcgccatgcc atgcgaattg actacttctc tgaaggtggt ttgcagtcag aactgtaaaa
    15181 agaatatcca aacaatatat tgcaccgttt tgccgtgagc agaacggtgc tttttgtaaa
    15241 atggtaagca ttgtagagga atgaaacgat gaagcgattg aacgctgtaa tatccgcagt
    15301 tgtgttcttg ttttttacgg gctgttcttc ctatccccac caaagctggc tggaagatag
    15361 aagcctcgat aaaatcgcag atgatagggc ttcagaaatt gttacagcat tagaaaatgg
    15421 agatcaagtt gcaattagat ccatgttttc taatcaggca ttgaacgaag ctgaagaatt
    15481 agacgattct attttatctg caatagaata ttataaaggt aatttggtat catctaatgg
    15541 aacgattgca accgatgaaa gtcagaatgg aaaaaagaaa acatttaaaa tcagggcaga
    15601 ttatacgata ttgactgatt tagaaactta taatttatat tttattgaaa agtataattc
    15661 ggaagatgag aatgaaaatg gcctatattt gatttggttg tctaa
//
LOCUS       DAWBGR010000041        15584 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1235_length_15584_cov_3.621160, whole genome shotgun sequence.
ACCESSION   DAWBGR010000041 DAWBGR010000000
VERSION     DAWBGR010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15584)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15584)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15584
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1235_length_15584_cov_3.621160"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4451 a         3449 c         3863 g         3821 t
ORIGIN      
        1 taatgattga atataatctc ggtgttcata ttgaaacaat atatgagttc aaacagattg
       61 atgatgcttt ctttgacgat gatgactaaa aagtataggg aggaatccat atgcggcaag
      121 cacaacttgc acgaaagacc aaagaaacag acatacaggt agccgtaaaa ctagacaaca
      181 taggaactgc caacatcgat accggcatcg gcttttttga ccatatgcta acagcactgt
      241 ctgttcacag cggcatcagc atggacattg ccgtaaaggg tgacctctat gtagacggac
      301 accatactgt agaggacacc ggtattgttc tgggaaaagc actgggcgag gcactgggcg
      361 ataagtctgg tattactcgt tacggcagtg catttattcc catggacgaa gctctggcat
      421 tttgcagtct ggacatcagc aaccgtccgt ttttggtatt tcaggggacg ttcaccaatg
      481 ccatgatcgg acagtatgat gcctgcctga cggaagaatt tttccgtgcc tttgcattca
      541 acgccggcat tacgcttcat gtgaatatga tgtatggctc taacgatcac cacaagtgtg
      601 aggcagcatt caaagccgtt gcccatgcct taaaaaccgc ggtaaccccg ctggaagcag
      661 gaaaaacctt gtccacgaaa ggagtgctgt aaacatgatt gccattatcg actacggtgc
      721 cggcaatatt ttcagtgtaa aaaatgcact ggactatctg ggattagctg gcgaactgac
      781 ttctgatccg gaagcaattc gcaaagcaga tgcattgatt ttgcccggcg taggggcatt
      841 ccccagtgcc atgcagatgc tgcacgacag cggattgacc aacatcattc aggaagaagc
      901 tcacaagaaa ccttttctgg gaatttgtct gggtatgcag ctgatttttg aaaaaggata
      961 cgaatttgaa gaatgcaacg gtttggggct gatccccggc aaagtagaaa aaatcgttgc
     1021 tccggatatg gtgatcccac atatgggctg gaacaagctg gaaatgcagc atgactgtcc
     1081 gattctccga aatatgccgg aagaatccta tgtgtacttt gttcactcct atcaggcggt
     1141 agtgccagac gaatatctgg cagcctatgt ggaatacgga ccagcaaaaa ttccggcaat
     1201 ggtctatgac ggaaaaacgg tttacggagc ccagttccat ccggaaaaaa gcggagagat
     1261 cgggctgcaa attctgcgga actttggagg gctgattcaa tgattatttt accagcaatc
     1321 gatattaaag acggcaattg tgttcgcctg ttcaaaggtg atttttctac agtagaaaaa
     1381 gtagcagcgg atcacatgga aactgccaaa ggatttgaag cggcaggagc atcatggatt
     1441 cacatggtag atctggacgg tgccaaagag ggcagaccgg taaaccagca tatttatgaa
     1501 gatgttgcag caaagaccgg attgaaagta gaagtcggcg gcggtatccg aaatctggaa
     1561 accattgaca cttatctgaa aagcggtgta gaacgtgtca ttctgggctc cgcagcattg
     1621 aaaaatccac aactggtaaa agatgcagta caaaaatttg gcagccagca tattgcagtg
     1681 ggaatcgatg ccaagaatgg catggttgcc acagagggct ggctggaaga atctgacaca
     1741 gattatatta cgctggcgaa agaaatgatt gcagtaggcg tacaatattt tatcgtaacc
     1801 gatattgcga aagacggcac gctttccggt gtcaatctga aacagctgaa agacttgcga
     1861 gatgcaacca acagcaattg caacatcatt gccagcggcg gtgtgcatac aattaaagac
     1921 attcgcggtt gtaaggaact tggactgtat ggcacgatct gcggcaagtc gatctataaa
     1981 ggcacactgg atctgaaaga agccattgct ttggcggaag gggtgtaaca tatgctggca
     2041 aaacgaatta ttccttgtct ggacgtgcga aacggaaaag tggtcaaagg tgtaaacttt
     2101 gagggaatca aagatgttgg cgatccggta gaatgtgcct acgcctataa ccaacagggt
     2161 gcagatgaaa ttgtctttta cgatattact gcgtcttacg agggcagagg cgtgatgctg
     2221 gacgtggtac gggaaacagc aaaaaaagtt ttcgttccgc tgaccgttgg cggcggcatt
     2281 aaaaccgtcg atgacattcg tgaaacactc cgtgccggtg cagataaagt ttctgtcaat
     2341 tcacaggcag tgcaaaatcc tcagcttatc aaagacgggg cagacatttt cggctgccag
     2401 tgcatttgcg tgggaattga tgccaagaaa gtcggcgaaa atcaatggac agttttcatt
     2461 aacggtggcc gtgtagacat gcacttggat ttgatcgact gggtaaaaca gtgcgaacag
     2521 ctgggtgccg gagaaatctg cctgaactcc attgacacag acggtgttcg cggcggattt
     2581 gatttaccaa tgctgaaggc ggtttgtgat gctgtacaaa ttccggttat tgccagcggc
     2641 ggtgccggaa agttgtccga ctttgcagaa gcatttctga aaactggttg ttccgcagca
     2701 ctggcagcat cgttgttcca tttccgcgaa ctgactgtac aggaagtcaa ggcagactgc
     2761 cgtgcaaagg gaattcctat gcgataacga tttgaggaga acattgcact atgatttttc
     2821 tcattttctg cgtactttgt attcctatta ttcaactgat aggtggctgg tgcatgcgat
     2881 accacacacc aaagaaaatc aatagctgga ttggctatcg caccacaaat gcaacgaaaa
     2941 atgcagatac atggaaactt gccaatcagt attgcggcaa gctttggctg atgatcggaa
     3001 cagtttcgct gattctttct gcgattatta gttttagctg tctgttcttt tccaaaaaaa
     3061 ttgccgccat tttgataccg ggaatgatta gcgtacagac acttgtactt ctgctgacaa
     3121 ttccttttgt agaagccaaa ttaaaaaggg agaacacttc acatgataaa aattgacatc
     3181 aacgatctgg acaaatattt ccagaaaggt gaactgattc cggcaatcgc atttgatgtg
     3241 gatactaaaa ccgtactgat gctggcatac atgaatcggg aaagcttacg gaaaacactg
     3301 gaaaccggat acacatggta ctggagccgt tccagacagg aactttggaa caagggtgcc
     3361 accagcgggc acttacagaa agtcatttct atttacagcg actgcgacaa tgatacgctt
     3421 ttgctgaatg tcaagcagac cggtgtggca tgccataccg gctcatacag ctgctttttc
     3481 aatgaaatgt accaaagcga agaagactga cattttagaa aacgctacat atagctgcat
     3541 atataagata tgcatacaaa tctgtcagaa caggctcttt cttaatccaa gtcgaaaaat
     3601 gccgtgcagg acaaatcctg tgcggttatt ttttggacgg atagtcacaa atgggagaac
     3661 tcctgaatac actgtagaga agcatttatc atgcttgaca gtgataaaag gaggtttcgc
     3721 tatgaaccag catcattctt tgccggaatc gtgtgcaaat acagttccga tcaaagtcaa
     3781 ccgcgtgttt gacagctgca gcgacagaga ttgtttctca aatatccaga ttctcatgga
     3841 cggaggagca ctgccggaga atattacaat ggtcaaaagc cggtgcgttc gtgtgtccga
     3901 catctgcatg aatattgaac cagtaccgtt caaccgcggg ttctattcta ttgacttgac
     3961 ctatacgttt cggatagaat tcatggcata tgaacgggca tgcagcagtc ctgtgctcct
     4021 gaccggaaca gcctatgcca gcaaaaatgc gattctctac ggcggtgaaa gcaacactca
     4081 gacatttttc agcagcggag aacagctggg agataccaat gcctgctgtg aaactgtaaa
     4141 tctgcccaca gcagctgtac aggtcgttgc tcctatcgca ttggaagcca gaattggttc
     4201 tgtttgcggc tctgacggca caacaccagt tcgcacagtt attatgacgc tgggactttt
     4261 ctcggttgta gaagtttttc gtcccgtaac cattctggtg cccacgtatg agtacaccat
     4321 tcccgcaaag gaatgtcata ccgacaccga atctccctgt gctgtttttg acaagattcg
     4381 gtttccggca gaagaatttt caccgtcggc actggacacc attcccaaag agaagtgctg
     4441 cggctgcaat gaactaacag aaaacgacga aaattctaca ttggaataaa cagacaacac
     4501 cgcccagagc tcgcctaagg gctttgcaca atttttatgt ggtgttgcct ttgatcttat
     4561 ccaacgcgta caggcaaacg ggcggcagtt ttgtcgcccg ttccttattt caaaggggtt
     4621 tctcacatgc gaaaacaagt ttaccggaaa cgccgtactc atatccgttc cctgctcctt
     4681 tttggaacgg ctatatgctt actgctggca tttctgcttc gcatagacca tgcgatccgt
     4741 cccaatctct gtgcagtttg tgaaagcgaa accaaacagt ttaccgccgg actaatggca
     4801 gacagcattg cagaagtgtt gcaagatcaa cagatgaaaa atgtcagctt tgctgaactg
     4861 acatatgata ccgccggcaa tgtcaccaca atggaaaccc gaacagacca gatcaatcgg
     4921 ctgcaatctg caatctaccg caatgtacag cagcatttgg aacagtgccg tgatgcagaa
     4981 cttgaggttt ctctgggaac tgcatctgga gtctggattt ttgccggacg gggtccgcat
     5041 gtttctgtgc ggcttctgcc ggtagggcat gcttccgtga agctcatcag tacaatagaa
     5101 tctgccggca tcaaccaaac ttgccacacc atacgagcag aggtaactgc ggaagtacag
     5161 acagccattc ccttcgcaca gaccacaaca aaaaccacct atgcatgtct tctctcggaa
     5221 actttaattg tcggaaccgt gccggattcc tacatggaat ttgaaagcac gtcggattct
     5281 ggcgaaaaac aggatttcag agaataaagc atgaaatatt tgtaaaaggt ctagcatatg
     5341 acacgttttt gtggtataat aaattgtgtg ccaaaaaaaa tgcagagccg ctttctgtgg
     5401 ctctgccaca caaggagtgt gaccgaatgg acgaagcaac agcaaaacag cgtatggcgg
     5461 aattacaaca gcggctagaa cgctataacg aagaatatta tgtacaggat gccccgtctg
     5521 taacagacta tgaatacgat atgcttctac gggaactgga aacattggag cagcagtatc
     5581 ctcagtttgc cgcagctgat tcgccaacca aacacgttgg cggagcagcg tcccgcacat
     5641 ttgaaaaggt gccgcacacc gtacagatga tgagcttgca ggacgtattt tcctatgaag
     5701 aaatgaacgc ttttgtacaa aagtgtcagg aggaactgga gaatccagag tttagcgtag
     5761 agccgaaaat cgacggatta tccgtatcgc tggaatatac caacggcgta ttcactcgtg
     5821 gttccacccg cggggacggc tttgtagggg aagatgtaac agcaaatctc atgacgattg
     5881 ccaacattcc caagaagctg gaacacgctc cggcatttct ggaagtccgc ggcgaagtat
     5941 atatgccgcg aaaaagtttc gctgcattag tggagcaaca ggaactgaac ggagaaacac
     6001 cggcaaaaaa tccccgcaac gcagccgctg gttcactgcg gcagaaaaac gccgtagtca
     6061 ctgcacaacg cagtctggac atctggattt tcaatatcca gcagatcgac ggagaaattc
     6121 tttcagaaca cattgaatcg ttagagtatc tccggaagtt aggcttccct acagttattc
     6181 cgcacagcaa acctttacga accgcagagg aaattcgtgc ggaaattgct gcaatcggtg
     6241 aagctaagct gcattacgcc tatgacacag acggcgtagt tgtcaaagtg aattcctttg
     6301 cacagcgaga agaaatggga gcgacttcaa aagtcccgaa gtgggcagcc gctttcaagt
     6361 tcccgccaga agaaaaagaa accacactgc gggaaataca gttgagtgta ggaagaaccg
     6421 gtgtgattac accagtggca attttcgatc cggtacaact tgccggaacc gaagtagcac
     6481 gtgccactct gcacaatcag gattttatca ccgaacgaga tattcgtctg ggtgatacca
     6541 ttgtggttcg taaggcaggg gagatcattc cggaagtact tcgtgtcaag tgtcatgcag
     6601 aaggagcttt accgtatcag ttgcccaatg cctgtccggt atgtcatacg ccggcagtac
     6661 gcgatccgga agaagctgcc attcgctgcc cgaatccaga ttgtccggca cagttgatga
     6721 aacgcatgat ccatttcgtt tccaaagatg ccatgaatat cgacggactg ggaccgcaga
     6781 atctggctgc cttgcaggaa aatggtctgg tgcattctgt ggcagacttg tattcattga
     6841 caaaaaagga actgaccgaa ctggaacgct ttgcagaaaa atctgcggaa aatctggttc
     6901 gtgccattgc cgcttctaaa gaaaattctt tggatcgtct ggtatttgca ctgggtattc
     6961 gcggcattgg ttcccgtgcg gcaaaattgc tctgtgaacg attcccgact atggacgctc
     7021 tgctgcacgc aacggcagag gaaatacagg aaattgacgg tttcggagaa accatggcag
     7081 aaagtgttgt aaaagcactg tcagagccgc atatgcttca actgattaca cacctgcggg
     7141 aagcaggctg taatatgacc taccacggaa cggtggtaga agatcatcgc tttgctggta
     7201 tgacctttgt actgaccgga acgctttcta ctatgaaacg cagtgaagcc aaagaattgg
     7261 tgctgcgtta cggcggaaaa gtcagcggca gtgtttcaaa aaagacaact tttgtggtgg
     7321 caggagaagc cgccggcagc aagttggaca aggcaaacga actgggtatt ctggttctta
     7381 gcgaagaaga atttcgtcag aaaattttgg agaacgcaga aacaaaatag attcttgcgt
     7441 actctgtgaa aggaacgata accatgaaaa ttgatattgc acatattgca aaactttccc
     7501 gactgcatat cgagccggag aaaatggaca agtttgaaaa agatatggct gccattgtag
     7561 atatggtaga ccgtctgccg gacgtggaag acgaactgac actggacgca gaacacccca
     7621 tgaaattgcg ggaagatgtt gcagtggaac ataaattccg ccgtgaagaa atgctggcaa
     7681 atgcaccagt ggtacagtct ggctgtctgg ttgttccgaa aacggtagaa taaggaggag
     7741 atacacatga agctgtatga gaaaaatgcc gcagaactgt ctgcaatgct gcaaaaaaaa
     7801 gaatgcagtg cagttgaact gacgcaggac gttctggaac gtatcgaaga aaaagaatct
     7861 gctgtcggtg cctatgtaac acgttgcgaa gattgtctgg atcgggcaaa ggcagtagac
     7921 gatgcacgtg ctgccggaaa agaactgcac ccgctggctg gtattccaat tggcatcaaa
     7981 gacaacattt ccactaaagg aattcgtact acctgttctt ccaaaatgct tggaaactat
     8041 gtgccgcctt ttgatgcaac agtaatgcag ccggttcgtg aagctggaat ggtgatcacc
     8101 ggtaagatga acatggacga gttcgccatg ggttcttcca cggaaacttc ctatttccac
     8161 actacacata atccgcacaa tctggagtgc gtaccaggcg gttcgtctgg cggcagtgcc
     8221 gcagcagtag ctgccggtga agcaatcctc acactgggtt cagataccgg cggatcgatt
     8281 cgtcagcctg cttccctctg cggtgttgta ggactgaagc cgacctacgg cagcgtttct
     8341 cgttatggtc tggtggcatt tgcttcctct ctggatcaga tcggtccgtt cggaaagtcg
     8401 gtcaaagatg ttgccctgct gcaaagcctg attcagggac atgacaacat ggacgccact
     8461 tctgcatatc ggacatacac taatctgtat gacaatctga acggcaacgt acagggtctg
     8521 cgtatcggtg ttccgaaaga atatttcggt gagggtatcg atgcagaagt caagactgct
     8581 gtgcatgctg cgatccagct gctggagcag aacggtgcta ctgtaaagga aatttctctc
     8641 cccagcacag aatatgcgat caatacttac tacatcattg cttctgctga agcttcttcc
     8701 aatctggcaa gatttgacgg tgtaaagtat ggcttccgtg cagaaaatta tgatggtctg
     8761 acagacatgt atgaaaagac cagaagtgaa ggttttggcg atgaagtaaa gcgtcggatt
     8821 atgctgggca cattcgtgtt aagttccggt ttctatgatg cgtactataa aaaagcaaaa
     8881 ctggtacagc gtcgcatcgg tcaggaattt gcagcagcat ttcaggattg tgatgtaatt
     8941 ctgacaccaa ctgcaccttc tgccgcattc aagatcggtg aaaatattgg tgatccgttg
     9001 aaaatgtact acagcgatgt ctgcacagtc actgtcaaca ttgcaggtct gccggctgtc
     9061 agtgttccct gcggaaagtc tgcttccggt ctgccgctgg gtatgcagct gatcggtaac
     9121 aagtttgcag aacagaccat tctgaacgca gcatatggtt atgaacagct ggtcggcgga
     9181 tttcacgcca ttgcagaaac cctttgagca ggaggaagaa atcaatgaaa gattatgaaa
     9241 ttgtcgttgg actggaagtc cacgcagaac tgaataccaa atctaaaatt tactgcaact
     9301 gccgtaacgc atttggtctg gaagtcaaca cacaggtttg ccctgtctgc atgggcatgc
     9361 cgggtacatt gccgacactg aatgaaaaag tggtagaata tgcaatcaaa atgggacatg
     9421 ctatgcactg ccagatcaac ggtgtttgca aacaggacag aaagaattac ttctatccgg
     9481 atctgccgaa ggcataccag atttctcaag cagaagtacc gctttgcgga aagggttatc
     9541 tggacgttat ggtagacgga cagatgaaga gaattggcgt aacccgtatc catatcgaag
     9601 aagatgccgg aaagctgctg catgacgagt ctttctctgg ttccctggtc gacctgaacc
     9661 gctgcggcgt gccgctgatc gaaatcgtat ccgaaccgga tattcgcagc agtgccgaag
     9721 ccaaggcata tctggaaacc atcaaatcaa ttttgcagta tctgaacatt tcagactgca
     9781 agatgcagga gggttccatt cgctgcgatg tcaatgtttc tgttcgtgaa aaaggtgcaa
     9841 ccgaatttgg tacccgctgt gaaatgaaaa atgtcaacag tttcagtgca gctgttcgcg
     9901 gcatcgaata tgaggcaaaa cgccagatag aaattctgga agccggcggt accattgcac
     9961 aggaaacccg tcgttgggac gatgtcaagg gtatgagcat tgttatgcgt tcaaaggaag
    10021 atgctcagga ttaccggtat ttcccggagc cggatctgct gaccattgta gtgccggaag
    10081 aaaagattca ggcactgaag gaaagtattc cggaattgcc gaatgtaaag ctgcagcggt
    10141 atgttttcga cttcggtctt tcccagacag atgccacact gattgcagaa gacatttctc
    10201 gttctaagat gttcgatgcc tgcgttgcac tgggcacctg caagcccaag agcatcagca
    10261 actggattct ggcagatatt gcaaagtata ccaacgaaac cggaaagtct attgaagaaa
    10321 cagctctgac accagaacat ctgacttctt tgattgagca ggtggaaaaa ggcgttatct
    10381 ccaacgcttc tgcaaagaaa gtgtttgctg taattgtaaa agaagataag gatccgcttg
    10441 ccattatcga agaaaagggc atgaagcaga attcggacac cggcttcttg gaacagctgg
    10501 caaaggacgt actggctcaa aacgaaaagt ctgtaaccga ttacaaaaac ggaaaaacca
    10561 atgcactggg ctatctggtc ggtcagtgta tgaaggcatc taagggcaag gcaaatccgg
    10621 gtattgtcaa ggaaattgtc accaaaatgc taaatgcata atcacacatt cgaaagcaca
    10681 tgatagaatg cattttctat ttcatgagaa tacattctaa aaaatcaagg caacagcata
    10741 cgatttgtat actgttgcct tgatttgcaa ctggattcag tttaaagctg tactttggga
    10801 aattcttttg tcatgaacgg cggtggcggt gtctgcactg tcaatccatt caaatcggca
    10861 aggcaagaat ccgcatctgt ttccaactgt aattgatatg cccacagcaa ctgatgctta
    10921 cagcggcagg cacggttttc ccgcatgttg ccatacttgg tatctcccag aatcggcgtg
    10981 tgaatatgtg ccagatgtgc acgaatctga tgcgttctgc cggtaatcaa atccactgaa
    11041 agtaatgcat gacgttcatt ggaagcaagc acctcataac cagtgatgat ttcttgaaat
    11101 ccttcttccg gtgtgtcacg aaccgtgact gtattatggc tggctgactt cttatgccag
    11161 ccgtaaagcg tcgctttccg ctcctttggt gtaccgacag tgacacagag atattttttg
    11221 tgtaccttgt tttcacgaat caaacggttc acttcccgca gtgccgcagc agttttggca
    11281 gcaatgacaa tgccaccagt atttcggtct aaacgattgc agagtgccgg tgtaaaactt
    11341 tgttcctgct ccggctgata tgcaccgttt tcgtacagat agtgcagtac gcgattgata
    11401 agagtatccg gaatctgtcg gctgtcacaa tgcaccacca gaccaactgg cttatttacc
    11461 agcaaaattc gctcatcttc gtagaggatt tccagttggt tcggtgcctg caaaaattcc
    11521 ggaacaccac tgcgttcttc ttctgttttg atagcaaaat aagaatcgct gacaaacagt
    11581 gtcaccacat cgccggtcac caaccgctga tctggtgtac agcgttttcg attcacttta
    11641 atgtgcttgg tacgaatcca cttgtagcaa agactttttg gcagttccgg caacgttttt
    11701 tgtaaaaagc gatccagacg ctggtcgctg tcgttttcct gtatgcaaaa ttctctcatg
    11761 gttgtaaatt gctgcgaaag cagcactcct ttttctgtta ttgattctat tatagcatat
    11821 ttttgcttaa aagaaaaggg ctataagcgt gcacacatga atttttatgg caacaccgta
    11881 caaagcttgt gtgtaagatg cgtagtcaga tttttcggca atatgacagc tttgtgtagt
    11941 gttgccttgt attttacctg atgataggag gctttatcat ggaacagtct aaaaaagaaa
    12001 tccagaatct gaaacaacct gcggacaagc ggaacgtttc gaattcggat actgctgttt
    12061 ccaagaaaaa acacagtcat gccggtcatc gggaacgcat gcggcttcgt tttcaaaaaa
    12121 gcggcttgga aagttttcag ccacacgaga ttctggaatt gttgctattc tatgctttgc
    12181 cgcgagtaga cacaaatccc attgcacatg atttgattgc agagtttcat tcactttccg
    12241 gtgtgctgga cgcggatata caggatttaa aacgtatcaa tggcatcagc gaaaacgcag
    12301 ccatatttct gaagttgctt ccgcagcttt gccagcagta ccagttggat aaactgcggg
    12361 aacatgttgc gttagacagc acacaaaaat tgtgtgccta tgtgcaggca aaactttccg
    12421 cgacagtgga agaaaaggtg cttctgctct gtctggacga gcacttgcat ttgctgcact
    12481 gcgaaacaat ctccaccggc actgcacaaa gagccgcact ggatacacat cgcattgtgg
    12541 aatgtacaat acgcatgcgg agcagccgtg tagtgctggc acataatcac ccgcagggac
    12601 aggcagcctt ttctgatgct gatctgtttg ccacccaaca gctgcggcgg ataatggaca
    12661 gtatgcaaat acaactgttg gatcatattg tattgggaaa acagggagat acaatttcca
    12721 tgcggcaagt catgggctgg agcgattcat aaaatgcttt gaaggaggga accaaatgga
    12781 tcattttgat ttgcatgcac cattttcgcc taccggtgac caaccggaag cgattgccaa
    12841 actggtacag ggaattcggg acggtgacca cgagcagact ttactgggcg ttaccggttc
    12901 cggtaaaacg tacaccattg ccaatgtcat cgcacaggtg aacaaaccga ctctggtgtt
    12961 ggctcacaat aaaattctgg cagcacagct ttgttcagag tttcgggaat ttttcccgaa
    13021 taatgccgta gaatattttg tgtcctatta cgattactat caaccggaag cctatattcc
    13081 cagcacggac agctatatcg aaaaagactc ctcgatcaat gacgagatcg acaaaatgcg
    13141 gcacagtgcc acagcggcac tggcagagcg aaaagatgtc atcattgtcg ccagcgtttc
    13201 ctgtatctat tccctgggca gtccggaaga atatcgttcc atgatggttt ctattcgtgt
    13261 gggaatggaa aagtccagag atcaactgat tgaccaacta gttgcaattc aatatgaacg
    13321 aaatgacatc aattttgtcc gcaataaatt ccgcgttcgc ggcgacgtag tagaaatctt
    13381 tccggcatct tccactgaca aagcaattcg cgtagaatat ttcggagatg aaattgaccg
    13441 catctgcgaa atcaatgtgg tttccggaga agttacagca accatgcagt atgcggcaat
    13501 tctgccggca tcgcattatg ttgtgggaag cgaaaaagtg accaaagctg tcaaagaaat
    13561 acgtcaggag atgctgcaac gggtggcgtg gttctccgcc aatcacaaac tgatcgaagc
    13621 acagcgaatc caacagcgta cacagtacga catggaaatg ctggaggaaa tcggtttttg
    13681 cagcggcatc gaaaactatt ctcgtgtatt agccggcaga ccggcaggca gtgtacccta
    13741 tacgttgtta gatcactttc cggaagattt tctattggtg gtcgatgaaa gtcatgtgac
    13801 actgccgcag gttcgtgcca tgtacgccgg cgacagagcc agaaagcaaa cgctggtgga
    13861 ctatggattc cggctgccca gtgcgttcga caaccgcccg ctgaatttcg aggaattcta
    13921 cagccatgtg catcaggcaa tctatgtcag tgcaaccccc ggaccatttg aaaaagagca
    13981 ttctgtacag accgtagaac aggtgattcg ccccaccggt ttgctggatc cggaaatcac
    14041 tgtgcgtccg gtagacggac agattgaaga tttgttttca gaaatcaatc tccgtgcaga
    14101 aaaaaatgag cgtgtacttg ttacaacact gactaaaaag atggcagaag acctgacggc
    14161 ttatttggaa cgtatgggcg ttcgggttcg gtatctgcac catgatatag atactgtaga
    14221 acgtatggaa attatccgcg atctgcggct gggagaattt gatgtgctgg ttggcatcaa
    14281 cctgctccgt gaagggctgg acattccgga agtttctctg gtagccattc tggatgcgga
    14341 taaggagggg ttcctgcgaa gtgaaacctc tatgatccag acaatcggac gtgccgcacg
    14401 aaatgcacac ggacaagtta tcatgtacgc cgacgagata accggttcta tggaacgggc
    14461 aatcacagaa acagaacgcc gtcgtgccaa gcagcaagcc tataatgaag cacacggcat
    14521 cattccgaaa accattcaaa aagatgtgcg tgatgtcatt gaaattacca caaaaacaga
    14581 agtggaaaag aaaacaactg gcaagaagtt acgcggcaaa gaaaaacgtg cattgatcga
    14641 acagctgact gcccagatga aagaagctgc aaaattgttg gaattcgaac acgccgcata
    14701 tctgcgggat aaaatcaaaa agctggagga ggaacaataa ttcatgttgg ataaaattgt
    14761 tgtacgcggt gcaagagcga acaatctgaa aaatattgat gtggagatcc cacgggataa
    14821 actggtagtc atgaccggac tttccggctc cggaaaatct tctctggcat ttgacaccat
    14881 cttcgcagat ggacagcgtc gctatatgga aagtctctcg tcttatgcac ggcagtttct
    14941 gggacaaatg gaaaagcctg atgtggacag catcgaaggc ttatcgccgg caatttccat
    15001 cgatcagaaa accacttcca aaaacccgcg ttccacagtg ggaaccgtaa ctgaaatcta
    15061 tgattatctc cgtctgctct atgcccgtat cggcattcct cattgtcctg tctgtggcag
    15121 agaaattaaa cagcagtctg ttgatgaaat tgtagaccgc attctggcac tggaggaggg
    15181 aacacgaatt caacttctgg cacccattgt ccgcgggaag aaggggcagc atctaaaagc
    15241 attagaaacg acaaagaagt ctggttatgt tcgcgttcgc atagatggaa atcaatacga
    15301 tttgtctgag caaatcacgc tggaaaaaaa taaaaagcat accattgaaa tcattgtaga
    15361 ccgacttgta gtaaagccga caatccgtcc gcgactgact gacagtctgg aaacagtgct
    15421 cgctttgaca gggggattag cagttgtaga cgtgattggc ggagaagaaa tgctgttctc
    15481 acaaagttat gcttgtccgg aacatggcgt ttccgtcgaa gaactgactc ctcgtatgtt
    15541 ctctttcaac aatccattcg gagcgtgtcc gcactgtacc ggtt
//
LOCUS       DAWBGR010000042        15465 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1248_length_15465_cov_4.472161, whole genome shotgun sequence.
ACCESSION   DAWBGR010000042 DAWBGR010000000
VERSION     DAWBGR010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15465)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15465)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15465
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1248_length_15465_cov_4.472161"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3157 a         4232 c         4405 g         3671 t
ORIGIN      
        1 acactaccct tttcacactt gaaattgggg cgtaaggtca gcggcgactc gccgcaaaag
       61 gaggtatttc tcatggcacc tattcgttta acggaatcct tctaccaccg cgactgtctg
      121 gaggtcgctc ccgatctggt ggggaagctg ctgatgcggc ggctgccgga cggcacgatt
      181 ttacaggaac gcattgccga aaccgaagcc tatcgcgggc aggaagatct ggcgtgtcac
      241 gcctccaaag ggcgaacgcc ccgcacagaa ctgctctatc gggaaagcgg cgtgatctac
      301 gtctacctct gctacggcat gcactggctg atgaatgtga tcaccggcga acgggaacag
      361 ccgcaaggcg ttctgcttcg tgccggagcc gtctacaacg gccctgccaa gctgaccaag
      421 tatctacagg tggacaaaca gttcaacggg gacagctttc tcacctgtcc ggaactgtgg
      481 atcgcagatg acggctttcg tccggctctg cgtaccgatg tgcgtgtagg tatcgactat
      541 gccggagaat actggaaaaa catgccatgg cgttggatcg ccgatgaaaa ataaaccgcc
      601 ccggtcgtaa accgatcagg gcggtactca aaacaaaaag cggcagtacc ggcacaaaac
      661 cgatactgcc gcttctattt tacaagctta cgctttcgtg ttcggggaaa ttagtcctcg
      721 tagtcagcgt cgttgtcctc ctccagcagt gcacgcatgg agatagagat ccgcttgttt
      781 tcgatgtcga tgtcaatgat ctttacccgt acaacgtcac caacggaaac gatgtccttg
      841 acattttcca cacgcttgtc agccagctga gaaatgtgga tcagaccgtc tacgccgtca
      901 atgatctgtg caaatgcacc gaacggagtg atggaaacga tcgttgcatc gatctcgtcg
      961 ccaaccttgt agtttgcctt gaacttctcc cacggattgt cttcctgctt cttgtagccc
     1021 agagagatgc ggttgttttc cttgtccaga tccttgatgt atacttcgat gtagtcgcca
     1081 accttgacaa cttcgctcgg gtgcttgatc cgcttccagg acagctcgga gatatgaacc
     1141 ataccgtcga tgccgcccag atctacgaat gcaccgtaac tggtcaggga cttgacttcg
     1201 cccttgtagg tcttgccgac ctcggcagtt tcccagaaag cttcctttgc agcgtccttt
     1261 tcagccttca gaacagcctt gatggagccg acagcccgtc tgcggtgttc gttgacttcc
     1321 agaatgacga aacgctgggt ggtctttacc agagaatcca tctctgcacc tctcggcaga
     1381 ccgctctgcg atgccggaat gaatacgcga acgccggcat agttgatgat aacgccgccc
     1441 ttgacagatg cactgacagt gccttccaga acagcttcct catccttggc cttgatgatc
     1501 tcctcaaagc ccttcatctc gtcgaccttc ttcttggaca gggtaacgat accttcctga
     1561 tcgttgatct tggtaacgat cagatccaga acatcgccga cagcgacaac gtcagacggc
     1621 ttcttggatg cgtcgtctgt cagttcgctg cgaggaacat aaccggtgtg cttggtgccg
     1681 acatcgacga tcgcttcaga atcgttgaca gagcaaacgg taccctttac cttctctccc
     1741 gtatgtattt ttttgaagga ctgatccaga gcttctgcga agttgatatc ctcctccacg
     1801 gcgttgttat ccatgatttc agtcatagtt tgttgaacct ccttaatgat atgtgccggt
     1861 gtggacgcgc cggcggtaat gccgatcttc tcggcgttac caaggtctaa agtgtaaagc
     1921 tcatccgaat tctcaatatg ataagaccta cagtttctgt tgcagacatg atacagcttc
     1981 acggtgttgg aactgtgtct gccgcccaca ataatcatta aatctgactt gcgagagagt
     2041 ttgtctgcgt cggactgccg cacctgtgtg gcgtggcaga ttgtatcgta aaccataata
     2101 ttcgggttgt ccttgggaag agcttctaaa cacttttccc atactaatat attatacgtt
     2161 gtttgtgcaa caattgcaag tcttttgcga gaattcacta attttttttg aaaaaaattg
     2221 tttaacgctt cttcactttc aaaaacaaag caattgcctt tacaatgccc cacaattgcg
     2281 gcaacttccg gatgattggc atctccggcg atcagaataa aatcgccggc tcgggtgtgt
     2341 tcttccacga tccggtggat cttcgccaca aagggacagg tggcatcgac atagggattt
     2401 ccagcctgtt ccagctggtc atagaccgca gcactgacgc cgtgggaacg gatcaccacg
     2461 cattcgtccg gctggagatc tgcgatgctg tccacaatgc gtgctccgcg gcgggtgaga
     2521 tcgtccacca cgttctgatt gtggataatg ggccccagcg ttgccacatg ggggtgctgt
     2581 tccagagcct gatagcaaag cttgacggcg cggtctacgc cgaagcagaa gccggcggat
     2641 tccgccacag tgatttcagc catacgcgat cattcctccg tagtgtcggc agcttgttct
     2701 gcctgttttt tctgttccag agtcgcccgt tttttgtcct gagcgatctc gatcgaagtg
     2761 cggtgtttcg gcacatcgtg aaggatcggg aacggcgggt tctcctccac catagatttg
     2821 atgctgtcca tgatgtcttt tcgcatggca tgcatttcgt ggggggtgga gtcggcgttc
     2881 agtccataat ctgcggcgta caggggcttg cccacgcgga cacagatgcg gctgcggaag
     2941 tgcaggttgt tggaatcaaa gaccagcccg atgggtacta ccggcttgcc gctgcgggca
     3001 gccagtacgc agacaccgct ttttcctctg ccgaccttgc cgtcggtgtg gcgggtacct
     3061 tccggaaaaa tggtcagatg ccgccgttta tccagccgtt tggtggcttc ggagagcatg
     3121 tcatagtcgg ggtgcttggc atctgcggtg ggaaaggctc ccatgagccg gataaaaccg
     3181 ccgaaaacgg ggttccggaa cagaggctcc ttggcgataa agttgaacgg gtgcggggca
     3241 gccattgcca ccagcaccgg atccagatag ctgcggtggt tggaggcgaa gatgacggca
     3301 gtgcccttcg ggacattttc ccgcccctcg aaagaaatgc ggtagtaaaa gaaaaatgcg
     3361 atccgcacca gataataggc gatgcggtaa aagaaaaaga caatgggata cggcaaaaaa
     3421 tgacctcctt tttggcagca gagccttttc aggcgttatt ccacgtccag acgctgttcg
     3481 atcagatcca tgatctcctc gcagacttcg tgaaagccca tggtggtgct gtcgatcagt
     3541 acggcatcat ctgcctgccg caggggcgaa acggcacggt gcatgtcgtt gtaatcccgc
     3601 tgcttgatgt cctccagaat ctggtcatag gtaggcttgt cgggcttgtc ctccatttcc
     3661 agataccggc ggctggcacg ttcttctgcg gaagcggtga gatagatctt caggtctgcg
     3721 tccggcagaa cgactgtgcc gatgtcccgt ccgtccatga tgacgttgta ttcccgtgcg
     3781 atgtccttct gggtttccag caggaactcc cgcacttccg gcagggcaga aacctcggaa
     3841 gctttcatgg aaacctccgg tgtgcgaatg ctttcggtca cattttcgcc gttgaggaag
     3901 atgcactggg tgccgccgaa gtatttcagt tccagatgac agtcgttctc cagatgcttc
     3961 agaaagtcct ttttcgtcgg gtactcggtg gagaacaggg caatcgcacg atacagtgca
     4021 ccggtgtcca cgtagacaaa tcccagctgt gatgccactt ctctggcaat actgctttta
     4081 ccggctcccg caggcccgtc aatggcgatg ttgatctttt tcataaaaat gacaaactcc
     4141 tttatgtatc atagaaatta tcatgcctgt tctgccattg ccagaccggc actataggca
     4201 gttgcaaagg caatttgtaa gttgaaaccg ccggtgtatc cgtccagatc gagaatctct
     4261 ccggcgaaat acagcccctc acagcacctg gaagccatgg tgcgggcatc gacttctttg
     4321 acagaaacgc cgccgcgggt gacaatggct tcttcaatgg gacggaatgc ccgcacatgc
     4381 agggaaaccg ccttgatgca gctgcccaga tgcagccgct gtgccttggt gagggcgttg
     4441 gctttggtgt ccggcgagat ccccgccagc cgcagcaccg gcaagatcag ctttgccggc
     4501 agcagggcgt ggagaatgtt gccgaggtcg cggttgggac tgtcggcaag atcccgctgg
     4561 atccgtttgt ccagctgctc cggagtcaga ccggatttca ggtcgatctc cagccggtag
     4621 tcctgtgggc tgccctttcg catcagggca ctggcactga gtaccagcgg accggacacg
     4681 ccgaagtggg tgaacagcat ttcgcccagt tcctcaaaga tgcacttgtt tccccgatac
     4741 agccgcagga tcacgttccg cagggaaagc cccatggctt cctgacacca tgtttcctgt
     4801 gtgaccagcg ggatcaggga gggcattggc ggcacaatgg tgtgaccggc tgtttctgcc
     4861 agacgataac ctgagccgtc agaaccggtc ttggggtagg acatgccgcc ggtggcgagc
     4921 agggtcgtcc ctgcccgata ttctctgccg tctgcggtgc ggacaccggt acatctgccg
     4981 ttttccagca gcagttctgc cgcgtcgccg tgcaccacgg ggattcccgc cagccgcact
     5041 gccttttgca gagcctgtac gatgtcctcc gcacggtcgc tgacgggaaa tacccgattg
     5101 ccccgttcgg ttttcagtgg aacgcccagc ttttcgaaat aagccatgac gtcctgcggg
     5161 gtgcagcggg aaaacgcact gtacaggaat ttcccgttgg cggggatatt tgccatcagg
     5221 gtatcgcggt cacagttgtt ggtgagattg cagcgacctt tgccggtgat gcgaagcttg
     5281 cgtcccagct gtttgttgtg ttccagaatg atacagcgtt tgccgtgttc ggcggcggtc
     5341 agtgccgcaa agacacctgc cggtccgccg ccgatgcata aatggtctgt ttccatatta
     5401 cctcactttg tttgatgcat ttcatgtaag cagatacagc ggctgctgca cgcttatgga
     5461 tagtatagca catcgtgctg aaaaatgcaa gggaaaatca caggcagcca tgaaaatcaa
     5521 ctttcatgat agcctcctct gaaaggggag ggggaccagc gaagctggtg gagggatttt
     5581 gatgaaactt tttcttgaac tatagatttt acggcaaaat ccctcagtca gcctgcggct
     5641 gccagctccc ttttcaaggg agcctgaaat aagcctccct tgaaaaggga gggggaccag
     5701 cgaagctggt ggagggattc ttctgtggaa tcttttgctt aatgaaaaag acttacgaga
     5761 taactcctca gtcaagcctg cggctgccag ctcagcacaa ggcacgccct acgggtgcct
     5821 tttcaaggga gtccaattat gttgtaaaat gaagtgcgaa aacccctctg tcacttcgtg
     5881 acatctcccc tttcagggga gatttttttg cttcatggtt ttcttcccgt caaaaactct
     5941 ttctccacac cgacggagcc agcagtacca gaatggggaa gatctccagc cgtcccagca
     6001 gcatgtccgc ggacagcacc gccttggaaa agtccgacag gaacgagaaa ttctccatgg
     6061 ggcctacgct gttgatgccc gggccgatgt tgttggtgca ggtgatgacg gcagaaattg
     6121 ccgtgccgaa gtccatgtcg tccagcgaca gcagcaggaa cgaagccagc agcacaccca
     6181 gatacagaat gaggtaactc cacacatggt ggagtgcctc cggacggaga attttgttgt
     6241 cggatttgac ttggttcacg gagttggggt gcaccatttt ccgcatggca gcagagccgc
     6301 ttttccagag gatcacacac cgctgcacct tgaacccgcc gccggtagag ccgccgcagg
     6361 caccgataag catcagcagg atcagcagca tctgggagaa cagcggccac tggttatagt
     6421 ctgccgtggc aaagccggtg gaggtgataa tggaagccac ctgaaaaacg gcatagcgga
     6481 agcactttcc cacgctgtgg taaatggggc ggatattcag cataatgagt gcggtcgaaa
     6541 tggcaacaat gctgaaatat acccgcagtt ccgtgttctt ccgcacttcc cgaaaccgcc
     6601 gtatcagcat gtagtagtac agggtaaagt tcaccccgaa cagaagcatg gtcactgtca
     6661 ccacgccatc cagataagga gagttgtaat aggacaatcc gtcgttcttg atgccgaagc
     6721 cgccggtgcc tgcaatgccc atgccatggc atacgctgtc aaacaggggc atgccgccgg
     6781 tgaacagcag cacaatggtg ataaatgtca gggctccgta gatgaggtac aggtacaggg
     6841 cggagttttt gcccttgggc accagcttgc ctacctgcgg accggggcat tctgcctgca
     6901 tcagatacag ggcattcccc gaggagggga gaatggcaat ggcaagcacc agtacgccca
     6961 tgccgccgac ccagtgggtg aagcttcgcc agaacaggat cccccgcggc agagcctcta
     7021 tatcagtcag aatggaagaa ccggtggtgg tgaagccgga tacgctttcg aaaagagcgt
     7081 ctatgtagtg gggaatgctg ccgctgcaat aaaagggcag tgctcccact gcggaaaaaa
     7141 tgatccacat cagggcagca atgaccatgc cctcccgtgc aaacatgtac ttgtgcctgg
     7201 gacggtagaa cacccccggc agcgatgcca gagccatgcc gccgcctacg cccagaaaga
     7261 tcagcatgaa gaaatattcc cgatagtaca ggctcactag agccggcacc agaaacagtg
     7321 ctgaggagag cagcagtgcc ttggacagat agtggaatac aatgccttta ttcatactcg
     7381 ttcaccgcct agtccagaat gtctttcatg ctggagaagt ggtgctcctt ggaaacgatc
     7441 accacggaat cccccagttc gatgacagca ctgccgtccg gaatgatgat ctcccgcttc
     7501 cggataatgg cacagatcag gatattcttt ttcagctgca cgtcttttag cggtacgcct
     7561 accagatcgg ggatccgttc cttgacgcgg aactcgatcg cctccacctg atcgcctacc
     7621 agatgataca gggattcgat ctcgctgccg gcggtgctct ccagcgaacg cacgtaactc
     7681 agcacgttgc ccatggtcag ggacttgggg gaaaccacgc agtccagccc cagttcggag
     7741 gacaggtcga tgtagttttc ccgactgact tttgtgacta ccttggcgtt ggtgctgtcc
     7801 ttggcgaaca gggagataat catattctcc tcgtcgatgc cggtcagagc cacaaagccg
     7861 tccacttcca gtacgccctc ctcgatcagc agatcctgat cggtgccgtc gccgcagatg
     7921 acgatggcct tggggagcag atcggacagc tcttcacagc gttcccgatc tttttcgatc
     7981 agcttgactt tcatgcccac tgccagcagc tgcttcgaca ggtaatagcc cactctgccg
     8041 ccgcctacga tcatgacggt acgcaccttg tccttcatga agccgctggc acggaagaac
     8101 cgttcaatgt tccggtgcga agctgccacg tgaatgcggt cgccctcttg cagagcgaag
     8161 tcgccggagg ggataaacac tttctcgtcc cgctgtacgg cacagatgag gaatttcgtc
     8221 ttgatctttt tgtagagttc caccagcgtg atgttttcca gccacgggaa gtccggcagc
     8281 cggtattcca ccagctccag cttgcccttg ccaaagacct ccacctttgc cgcggagggg
     8341 aacaccagta cccgcatgat ctcgtctgcg gtggaaagtt ccggattgat gaccattgac
     8401 agccccagat cttcccgaat caggtgcatc tgctggtaat actccggcat acgcacgcgg
     8461 gaaatggtct ttttcgtgcc cagccgcttg ccgatcaggc agcagagaac attcacttcg
     8521 tcatagggcg ttgccgcgat gagcagggtg gctttgtctg ctccggcttc cagctgggtt
     8581 tccgccgttg ccccgttgcc ctcgatgcac ataacgtcca gtgtgttctg gctctttcgc
     8641 aagtgggttt catcacagtc taccacggtg atgtcgtgtc cctcctgcac cagcagggca
     8701 gccagattgc tgccgatctt gccgttccca ataattacga ttttcatagt tgattcacct
     8761 gttgttccac ccgttatttt cccatgagcc gccgtgtccg tgcggcaagc ttttcccgcc
     8821 gtgcctgtgt ggcggcggtg tcggtctgat agctgccgtc cggattctgc cggagaatgt
     8881 cgccctctct ggcatcgccc agaatctggc tgcgggggat ctctgtcatg ccgtcgtcgg
     8941 tttcgatcac ggcaatggtt tcttcgaacc ggtcaatgat ccacatcaga agcacctcac
     9001 ttttcagttg tcacggtgag attggagccg tcggacgaaa tggtcaccgt gccgctttcg
     9061 tctgtccgcc agatctgttc gccgcagtat tgctgcaggc gggataccgc ctcgctgtgg
     9121 gggtgtccgt agctgttgcc tgctccgcag gagatcaccg catagctggg ctgtaactgt
     9181 gccagaaatg catctgtgga ggattcacgg ctgccgtggt gtcccacttt cagcacgtcc
     9241 gcctgtatgc cgtccagttc gcctttttcc agcaggtctt tttcctccgg cttggacgcg
     9301 tcaccggtga agaaaaagct ggtgtccccg caggtgagat aggcacagat ggaatagtcg
     9361 ttcagagaac tgtagtcctc ggcgggagaa ggtatgatct ccagcgaagc cgttcccatg
     9421 tcaatggtca tggaggtggc aggtgtcagg atcagtccgt tggcttcgat gccgtccaga
     9481 aacttttcgt atgtacgggt ggtcggcacc atgctgtccg gcagctgggg gctcatggcg
     9541 ttgcagatcg tcaggtcact gttctgtgct gcgtagttta aaatcgtcgg gaacccgccg
     9601 atgtggtcgg cgtgggggtg ggtgccgatg atccagtcca gcttggtgat gccgtactgc
     9661 tgtaagtcgg cgagaatgtc gtctgcggca ctctgttcgc cggcatcgat gagcacagcg
     9721 gcggtgtcgg tgacgatcag ttcgctgtcg ccctgtccca cgtccagaaa gtggacggta
     9781 cagcttccgg tggcagagcc gccgccggtt tcggcttcct caccgccgaa cgcctgctgt
     9841 acggtgtcgg tgagatattg ttcggcatcg tccagcgaac agccgcccag caggggcagg
     9901 gctgccgcag ttaggatcgc cgcgattttt tggaagaatg ctttggtttt cgtggggata
     9961 cgcctccttt ggtgtgggaa ttccggtttt tgtcggcatg gctgcgttgg ctgtcggatt
    10021 tttgttccgg agccaccaga cagttcggct tggtgccgat gagatcctgc cgccccgcct
    10081 gcttcagtgc agacagcacc agctggcggt tctggggcag gtaatattgc agcaaagccc
    10141 gctgcatggc tttttctttc ggcgttttcg gcacatagac cggctccatg gtgtagggat
    10201 ccagttccgt gtaatacatg caggtggaga tcgtccccgg cgtggggtag aagtcctgta
    10261 cctgttccgg atgcatgccg ttgtcccgca ggaacaggga aagctttacg gcttcctgca
    10321 gagtgctgcc ggggtgagag gacatgagat agggcaccag atactgtttc ttgccgaggc
    10381 tcttggtgat ctcatagaac cgcttctgga acgcttcgta gcactcgata tggggcttgc
    10441 ccattttgtc cagcaccacg ttgctcacat gctccggagc cactttcagc tgcccgctga
    10501 cgtgctctgc caccagttcc ttgaagaagg tgtcgtcctt gtccgccata acatagtcga
    10561 accggatacc ggaacggata aacacccgct tcaccttggg gagtttccgc agcttccgga
    10621 gaatgcggag atactcgctg tgatccacct gcaacgcctt gcagggttct ggagcaagac
    10681 actttttccc gacgcacatg ccggatttcg cctgcttttt gcaggagggc agccggaagt
    10741 ttgccgtcgg gccaccaacg tcatggatat agcccttgaa gttgggcatt tccgtgatct
    10801 ttttcgcttc ttccagaatg gactcctcgc tgcggcaggt gatgcggcga ccctgatgca
    10861 gggcaatgga gcagaagttg cagtagccga agcagccgcg gttgtgggca atggagaact
    10921 gtacctcctc aatgcccggc acgccgccct gtgcatcgta catggggtgg ggcttccgct
    10981 gatagggcag ccggtatact gcgtccagtt cttcctgtgt caggctgact gccggcgggt
    11041 tctgcaccag catcttgtcc ccgtgccgct ggagcacggt ctttccggtg atctcgtcct
    11101 gctggtcgta ctgctggcgg gttgccttgg cgtatgcctt tttgtcgcgg cagacctgtg
    11161 cataatccgg acactgcacg ccgccccacg gggtgttttc cggcgaggtg agataacagg
    11221 tgccgcgaat gtccgtgatc tcctgtatgg gcacgccctc tgccagcagc tgtgccagtt
    11281 cggtgatctg gtgttccccc atgccgtaca tcagcagatc tgctccgctg tctgccagaa
    11341 tggacgggcg gacagtgtcg tcccagtagt catagtgggc gaaccgcctc agagaagctt
    11401 ccagtccgcc aatggcaatg gcagcatcgg ggtaagcctc ccgaattttc cggcagtagg
    11461 tgatgacggc acggtcggga cgaagcccgg ctctgccgcc gggggagtac atgtcagtgc
    11521 tgcgtttccg cttggcggaa gtgtagtggg caaccatgga atcgatgttg ccgccggtca
    11581 ccagaaacgc cagtctgggc ttgccgaatc gggtgaagtc ttttactgtg tgccagtccg
    11641 gctgggcgag aacggcgacg ctgtagccct gcgactgtaa caggcgggtg ataatggctg
    11701 ccccgaagct ggggtgatcc acgtaggcat caccggtaat atagacgaaa tccggctggg
    11761 atacgccggt ttccagaagt tcctgcatgg tgacagggag aaaattggtc atgtatttgc
    11821 tccttgaata ttgtgacatt attgcagtcg ccgcattttc cgttcggcaa actgctcctt
    11881 ggacatcagc actctgcggg gcttggcacc ctcatagggg ccgatgatgc ccttttcctc
    11941 cagttcgtcc atgatgcgtg cggcacgggc atagcccagc ttcagccgcc gctggaggga
    12001 ggaggtggag gcctgtccgg cttctacgac cacttcaatg gcacgttcga aaatgtcctc
    12061 gtcgctgccc ggttccggct cgccggaatc ttttcgctgt tcctttgtct ttccctctgc
    12121 cttggtgacg ggaatgttct gttctacctg ctccatgatc tcgttgctgt attcgctttg
    12181 ggtctgggac ttgataaagt ccaccacctt ttcgatctcc ttggtggagg tgaaacagcc
    12241 ctgtacacgc accggcttga tcttgccgtt gggcaggtac agcatatcgc cgtgtcccag
    12301 cagcttttcc gcaccgccgg tgtccagaat ggtgcgggag tccacctgcg acatgacaga
    12361 cagggcgata cggctgggga tatttgcctt gataagtccg gtgatgatgt ccgtggtagg
    12421 ccgctgtgtc gcaatgatga ggtgcatgcc tgccgcacgg gctttctgtg ccagacggca
    12481 gatcgcatct tccacttcct tggatgtggt catcatcagg tcggcaagct cgtcgataat
    12541 gactacgatc tggggcatag gctccagcgg actgttcggc tgggcagccg cggcgttgta
    12601 gtcgcccaga tcgcggacgc tgttttcggc gaacaggtta taccgccgct gcatctcctg
    12661 cactgcccag ttcaacgcac cggcagcctt tttcgggtcg gtgaccactg ggatcagcag
    12721 gtggggaatg ccctcataga cggtgaattc gacgatcttc gggtcgatca ggatcagctt
    12781 gacttcctcc ggtgtggcat ggtacaggat actcatgata atggagttgg tgcagaccga
    12841 cttaccggaa ccggtggctc cggcgatgat catatggggc agccgtgcaa tgtcgccgat
    12901 gacggcgttg ccggcaatat cccgccccac ggcaaacgcc agcttgctct tggaactgcg
    12961 gaagctgtcg ctctccagaa tctcccgcag ggaaacagtg tccttgtggc tgttggggac
    13021 ttcgatgccg atcgccggct tccccggaat gggggcttcg atgcggacac cctgtgccgc
    13081 cagcgacagg gcaatgtcat ctgccagtcc ggtgatcttg gaaaccttga tgccggcggc
    13141 aggttgtatc tcataccgcg tgacggaagg accgcggaaa ataccggtaa tgcggacagt
    13201 cacgccaaag cttttcagcg tgtccaccag aatgcccgct ttttctttca tctcctgatc
    13261 taccgcaggg tcgccggcac ggctgacacc gttctgcaaa aagtcgatgc tggggatctg
    13321 gtagggacgt gccggaaccg tcggtgctgc cggagcagcc ggttctgacg ggctctctgt
    13381 ttctgcggca ggctcagcca gttccggcgg gtcctgcagc agttcatccg gtgatacgat
    13441 cgcggcgttg tccggctcac tgtccggaag gggaatgtca atgtccatgt catacgccgg
    13501 cgtatagggg gcatcgtcct gcggctggga atagctgttc cggatcatgt cgtccagttc
    13561 cgatggcagc ggctggtcgg cagccacaaa gtccggaatg tctgcgtccg gagccggttc
    13621 tgcggcagcc ggttccggct gtggctggac agtgcctttg cggtgtacct ttgcaatttt
    13681 cggggcgtgc tgttccggca gcaggaattc cggttcttcc tctgcggcgg catcttcggg
    13741 aatatcgtct gcccattccg gatcacggtt ccacagttcc ctgagtttcc ggaacggaaa
    13801 gcctatgtac caccacagtt gccggatact cagatccatc agccgcataa tggtgagaaa
    13861 gagcaggcag atgatgatga tctgtgctcc gggtgtgccg atgatccgca ggaacggata
    13921 ggcgaggaac gtactgattg ctccgccgct ggcgaaagcg ttttccgtgc ctgccacaaa
    13981 cagatatttg aagcagcgga aaaagccctt tttgccgatg tcgcccacga atatgatctg
    14041 gaaaaaactg ctcaccagca cggtcagccc cagactcagc agaagccgtt tggtgagatt
    14101 catgccggac tggcgttcgg tgaagtaggc agccgccatg agcaggatcg gcaccagcag
    14161 aatggaaaag ccgaaaatgc cgcggataat gcagtgcatg agataccagc cggcgttacc
    14221 gtggacgaat gccagcagaa aaaataaaat ggcactggtg aaaagtacca ctgactgaaa
    14281 tttggtgttg ccgttctgct gctggttcag ctttgccaga gcctcctcct tggaggtctg
    14341 tgcagcagaa gtgccggcag tctttttctt ggctgccggc ttctttttcg ctgcgggttt
    14401 tgaagtcttc tttttcgaag acgcagattt ttttgccaat gttacgcctt tctgaaaaga
    14461 actgcaatgc tgaaaattca ggttttcgcg tttccggacg gtcgcagttc ctgtgtttct
    14521 atccgatgat agagtgcatc aatggcatcg cggagagaac ccagccggtc gatcagcccg
    14581 cattccaccg cttctgcacc ggagatgact gttcccatat cggttgccat ttcctgtgtg
    14641 ttcagcatca tgttttccag ccgagccttg gagatgcggc tgtggctggt gatgaagcct
    14701 gtgatacgct cttgaatttt tgaaaagtaa atggttgtct gcggcacgcc cagcaccagt
    14761 ccgctggtac ggacagggtg cacagtcata gaggcagagg gcacgatgag ggaaacgtcc
    14821 gcactgacgg cgagagggat cccgatggag tgaccgccgc ccaccaccag tgaaaccgtg
    14881 ggggtctgca tgccggcgat gacttccgca atggcaagcc ccgcttccac atcgccgccc
    14941 atggtgttga gcaggatcag cagcccttca atgctgcggt cctgttcgat cgccaccagt
    15001 gccggcagga cgtgttcata tttggtagtc ttggtgtcct ccggcagcac gttgtgtccc
    15061 tccacctgac cgatgatgtt caggcagtgg atcaggtgct tggcgttggg tgtccggacg
    15121 ctgccggttt cttcgatcag ctgtgcctga tccagctgtt cctctgcggg ggacttgctg
    15181 tcagatggtg tcgtgttagc catagtgctg ctccttcggg taaaatatcg gaaacgtcct
    15241 ttttattttg tccaaaagaa cagcagttat gcagacaaca gacagggact tctgcaagat
    15301 gtccctgtac acctatatta tagcagattt tgtggaaaag tcaaggcggg gagaagaacc
    15361 gtcattaggc ggtggagggg ttctttcgtg aaatcttttt atgacgtttt cggcataagt
    15421 ggaaacccct cagtcagtgc tacgcactgc cagctcccct ttcag
//
LOCUS       DAWBGR010000043        15284 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1259_length_15284_cov_3.390242, whole genome shotgun sequence.
ACCESSION   DAWBGR010000043 DAWBGR010000000
VERSION     DAWBGR010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15284)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15284)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15284
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1259_length_15284_cov_3.390242"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8831..8840
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11824..11833
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4074 a         3628 c         3637 g         3925 t
ORIGIN      
        1 attgtgccgt cggcggtgag tgtccaggtc aggttgcctt cgttccagta ggttctgatt
       61 ttgcttgcat ctacgccgag ttgtccgctt gtcagcgtag agccgttttc aaggtatacg
      121 gtcagcttgc cgcagccata aaacgcaaaa tttccgatgc ttgtgacact ctttggaatg
      181 gtgatgcttg tcaggctgct gcaactgtca aacgcataat ctccgatgct tgtgacacta
      241 cctggaatat tgatgctggt caagtcagtg caattgtaaa acgcaaaatt tccgatgctt
      301 gtaacactct ttggaatgct gatgctggtt aagccgatgc aattccgaaa cgcaccctct
      361 ccgatgcttg tgacactacc tggaatcgtg atactggtta agccggtgca attctgaaac
      421 gcaccctctc cgatgcttgt gacatcgttc ggaatcgtta cttctcccga acaagctgtg
      481 ccgtcgatca agatcccatt tacgatcaca agtgaatctt catgctgttt gtttgttaac
      541 caaagtgtgc cagaaaacgc acaatctccg atgcttgtga cactactcgg aatttcgatg
      601 ctggttaagc cagtgcaatc gtaaaacgca ttatctccga tgcttgtaac gccatcttca
      661 attacaactt tttttatgtt gtctttcttc tgggttgcag ggctatcatt agagtaattc
      721 ttcatcgtcc ccgtgccgct gatggtcatt gtgccgtcgg cgacgagtgt ccagatcagg
      781 ctgtcttcgt tccagcgggt tacaactttg tctgcaactt caccgaaact ttcgcttgtc
      841 agcgtagagc cgccttcaag aagcacctca gtcagagcag tacagccgga aaatgcttca
      901 tctcctacgc tttcaacact ctttggaatg gtaatgcttt ttaagccggt gcaattccga
      961 aacgcatacc tttcgatact tgtaacactc tctggaattg tgatacctgt caagctgctg
     1021 caatactcaa acgcagaatc ttcaatgttt gtgacactct ccggaatcgt gatgtctgtt
     1081 aaactgctgc aaccggaaaa cgcagaattt ccgatgcttg tgacactatc tggaatcgtg
     1141 atgtctgtca agctgctgca accggaaaac gtattatctc caatgcttgt gacaccactc
     1201 ggaatttcga ggctagttaa gccgccacac ccggcaaacg caccatttcc gatgtttttg
     1261 acagtttctg gaatttcgat actggttaag ccgccacaat tgtaaaacgc aaaagctccg
     1321 atgcttgtga caccttctgg aatttcaatg ctttttaaat tgctgcaatt gtaaaacgca
     1381 gaatttccga tgcttgtaac gccctcttga atcacaactt tttttacgtt gtctttcttt
     1441 tgggttgcag ggctaccaga gtaccaacta taacccttca tcgcccctga gccgctgatg
     1501 gtcattgtgc cgtcggcggt gagtgtccag gtcaggttgc cttcgttcca gtaggttttg
     1561 attatgcttg catctacgcc gaggctttcg cttgtcagct tagagccgtt ttcaagaagc
     1621 acctctttca aagaagaaca atgattaaac acaccagctc caatgcttgt gacactactt
     1681 ggaatcgtga tgctggtcaa gttggtgcaa cccaaaaacg caccacttcc gatgcttgtg
     1741 acactacttg gaatcgtgat gtctgttaag ctgctgcacc aagaaaacgc atcacttccg
     1801 atgcttgtga cactacctgg aatcgtgatg tctgtcaagc tgctgcattc ccgaaacaca
     1861 ctatttccga tgcttgtgac actacctgga atcgtgatgc ttgtcaagct actgcaccac
     1921 gaaaacgcag aatctccgat gcttgtgacg ccatcttcaa tcacaacttt ttttatgttg
     1981 ctattgttgc gtgcaggact atccgtcatc tcccctttgc cgctgacggt cattgttgtg
     2041 ccgtcggcac tcagtgtcca ggtcaggtcg ccatagtgcc atgtctgcga tcccgattct
     2101 gtccctgcac ctgcgtcctc cgcactcacc gtgattcccc acgagatatt ttgcagggta
     2161 tcggtcggta cgtacagcag cgttcccgcc gccatgacta atgccgacga aagggcagcc
     2221 agccgttccc attgtttctt cataatatgc ctcctttgtt ttcggatata ttacatatcc
     2281 ttgatattcc tattataaca caatcgagta ttctttgtca agaaaaaagg aggatttttt
     2341 gatatttcgt tcaatttttg gaagttggag aggggagggt gttgtttcgt tcttgtagta
     2401 gtcaaggtca aacttctcgt gttttgtgag aaaaactgct ttgcacgcaa tctgcttaca
     2461 gatttttgtg ttgacatgtt tcacaaaata tagtataata ttgttgacag aatcatccgc
     2521 attgacgatg tactcttttg agatgtgcat cgatttcaag gaggtataaa tgtatgaaac
     2581 aatggattcg cgtaaaaaag gcattgctcc tgtcgttaat tttgctgatg gcttggctgt
     2641 tgccgctgtt tcaatggaat ggaacagttt tgtcggcagc ggcaatctct acagattatc
     2701 cggcacagct aatgcatctt gccagcaagg gcagcaccaa agttctgaca gcaaatggaa
     2761 cttctgacgg agcggcactt tctctgcaaa cgctgggcag tgatttatct gcatcatggc
     2821 ggttcgatcg tgtcggttcg gatgggaacg gtactttttt caaactagtc aacgcacagt
     2881 ccggaaggct tttgacgccg cgaaattata acgtatctga caaaacagat gtgattttgt
     2941 acggcagtga atccgcacaa tctcaacatt ggtatgttgt tcccgtgaaa caagatcatc
     3001 tgggcaatga tctatactat aaaattgtga attattctga tacttctctt gccttgacgc
     3061 agggaacttc cggcatgaca ttagccaaat acagcggaac agacaatcag ctctggctgc
     3121 ttaatgctga cggattacag ggttttgcag gatattgctt tgatgacaac accggcaaca
     3181 tcaaggcagg ggacattggt ggtctgttcg gcgaaatcgt agaggtttcc acttttgccg
     3241 atcttaagaa atatgctacc gctgatatac cttataccat tgttgtcacg gcaaatattc
     3301 gtgttaccgc actgcaaaag gattcctccg gacgcaacta ttgtccggac ggacgaattt
     3361 atgttcacag caacaaaacg atcatcggca gttacgcggc acatactatg tataatgtac
     3421 agttctgtac atcttccaat aacggaaccg gaaacaatct gatcctgaaa aactttgagc
     3481 tacagcacga tgcggagtcc aacggcaatg attccattgt tgtctatctc ggctctggac
     3541 agaacatctg ggtagatcac tgcacctttg taggccatag cgattataat acagcatcta
     3601 ccggactacc agactgggat aagtttctgg catgctgcta tgatgctgac tacacaacag
     3661 tttcagactg ctctttcgga cttcacgaat acggtgtgat attggggtat ccggcagatg
     3721 atgaaaactc ttataaaacc tataataatt atccccgcat gtccattatc agcaatcgct
     3781 ttgaaaaaac tctgacccgt ggccctgggc tgatgcgtta cggttatttt cattcgctga
     3841 ataactatgt gaaaacattc agcatggcat atactgtgca cacagcatct aagatttttg
     3901 cagaaaactg ttattatgaa gacggcggaa acgttatctg tgactggaac acagcaacct
     3961 atcccggctc ttatgcagaa acaggttcaa aatcggtgaa ctgcaagcga acaacgatcg
     4021 aggggtatgc acaggactgt acatggcgac caacttccaa ttacaaaacc atttctcgca
     4081 ctgcagatga ggcaaagttt tattgtgaga actacagcgg ctgtcaaaat gacagaaatc
     4141 atatgatgta tctgcgatat gcagttgctg gtgtgccaag tgcaagctat acagaatcac
     4201 ccagtgcacc attggcagaa ctttttgccg agggttctgc ttaccgtatt cgcaatgtca
     4261 actccggctt gtatttgcag gtagcaggtg ctgccgcaaa aaatggcacc aatgtgcagc
     4321 agtggggatc agatggaatc gcagttcacg acatctggaa attgtgcagt gccggtgaag
     4381 ggtactacta tctggtttct gcagtagggg acggcggaac atatgtgctg gacgttgctg
     4441 gaaaaaaggc cgcaaatgga accaatatag atatttacac ctataacggc ggtgacaatc
     4501 agaagtttat gctgacaaaa aacggggacg gcagctatca gattcgaacc catatttcaa
     4561 atggaaattc tgtggtagaa gtagaaaatg cctcccaaac ctccggtgcc aatgtgcagc
     4621 agtgggaagt caacggtgcg aactgtcaga attggatttt agagccgaca actgaccccg
     4681 gctgctccat gaacaccgat gtgatctata cctttgaaaa tgctggttct ggtctggtaa
     4741 tggatattac cgatggaaaa atgacagaca atacaaatgt acagcagtgg tcgtccaacg
     4801 gattgaattg ccagaagtgg acacttcgtg cctttggctc tggcaattac tattggattc
     4861 gctcacagca ggatagtcat tatgcattga aggcagaggg cagcaaaaac ggcggaaatc
     4921 tggcaattgc agcatggtcc aataaagata gcactcagct gttccggttt actaagaatc
     4981 tggacggatc ctacagcatt ttgacacacg cttctggtga ttcctgttat gtagaagtcg
     5041 ccgatgcatc cactgcaaac ggtgccaatg tgcagcaatg ggaaccgacc ggcagcagtt
     5101 gtcagaaatg gcagacaaag acagaaacca ctactgtaac aactaaggta actacaactg
     5161 taacaacaac gacaacgacc aaagcaacca caaatacgac aacagccgca gcaactagca
     5221 ccactactgc aactgctaca gaaccgcctg tgatttctgg tgacatcaat gcagacggca
     5281 aaacgaatct ggcagatgtg gtgctgctgc aaaaatggct gttgggattt cctgaaacaa
     5341 aacttgccaa ctggcaggca ggggatctga atgcagatag aattctgaac ggattcgatc
     5401 tttgcctgct gcgaaataat atgatatagc tacctgtaca cttttgtgca gtatttcctt
     5461 tgcacagagt gattcctgtt cgtacctgcc gaacaggcgt acagcagaat atcaattttc
     5521 cggcgatgac aaaagattcg cttactcatg ttccctcatg ctttccatga aaatcgtcac
     5581 gcatttccag ccgtcgctga gatgctgccg tctgtcctac ggagtgcagc agttctgtca
     5641 gcattttttg cataaatcgg cgggtttccg gctgcatgtc tgtccatgca cggagcacac
     5701 cagagagcgt tcctgcaccg ccgccgatca gatcttgtat ggtatgggca ttggtttcac
     5761 tttgcagaat gtggaaaatg gcatcggtca gcttttctcg ctcctccggt ttcatgccca
     5821 ggatccactg ccgcagcgag gaatccagca gcttactggt gttggtggta tcctcggaat
     5881 agtaccagtc attccggatc acacaccacg agtatggatc gtgctgcaaa aaaccttttt
     5941 gtgtgctgta aactacctga tatttctcgt catgctccag cagcatgcct actacagaag
     6001 cttgtggaac aaaggtatac actcgttcct gcaattgctg aaactgcggc tcagcaaaca
     6061 ggtctgtcac aaagcctggt ccgtcattgt tatagactgc acgaatccgc ggaaagtctt
     6121 tcggctgcat ttggacggct gcataaactg ccagattgcc gcctttggag tgtccgccca
     6181 catagacgtt ggaaaatccc agtgacaaaa tcccccgcag atacgccgcc gctgctgcct
     6241 gtgacggcac atagggcaga aaagacaagt tgaaatcttc ccgccagcca gtcagtgtgt
     6301 tgtctgtgcc acggaatgcc acataaacag tatcactgtc cagcagaaat gcagtggcgg
     6361 aaaattgctg ctgctgggtt tcgctgaatt gttctgcata acggaataaa cgcacttctc
     6421 cgaaacgccg gctggctgcg gtctgccgaa gtaggtcgcg gtcagtttct acccgcactt
     6481 tctgttctgc agtgggacgg tgttcccaga gccttgctgc atcccgcaga gaaatcgatt
     6541 ccgctgcggt aaaaccttct gcgatgccgt cccagttcag ataagacagg cagcagaaaa
     6601 tcagattgtc cacgtgacag aaaggcgatt gttgcatggt cagatcatcc cgccactggc
     6661 aataggtcaa gatatttgca tcatacgtgg ttttcattgc agtcacctca aaatccattt
     6721 tgttttagct tttgtaccag ctggctgtac cattcccgca cgtcaaagtc cggaatattt
     6781 tcccgattca ggtcgatcat atccccgtgg gaaactcccc gccgcttagt ttcaggcacc
     6841 atgagaaaag gtgcccactc cagttttgcc gatacaaccg gtaccaggcc gtcgttctct
     6901 ccagcttttc gccggttcag gaaatatccc agccacagcg ggaacggggc ggaaaatgca
     6961 ctgtgcatcc tggacatcac cgcctgataa tagacaccgg aaataggggg atgttccgta
     7021 tgaaattttt gacagttttc ggcagtcaat tcccgcacac cagccagaaa atccggattc
     7081 tgatctccca gttttgtaaa cagccggtta tactttgctg cgatccatcg tgccacacct
     7141 gccggcacct tttgcaatag atcgtccaca aaggcacagc catgatgcgg cgtattgatc
     7201 gttgtcagag aagccacata ctttgccgca ccatattcct gcatggcaca gcggcagtcc
     7261 agaccgccct tggaatgggc aatgacgttg accttttccg caccagtttc agcaataaca
     7321 gactgaattt gtgctgccag ttccttggca ctgacggaaa tcgattgtgc cgactgctgt
     7381 tttccgtaaa aaatagttgc tccgtttcga atcagttctg ccggaatccg tccccagtaa
     7441 ttgaaaaatt gccagtcccg aaaaaagatg ccgtgcacca gcaaaatcgg atacctggtc
     7501 ttgcagatct ctgattcttt tcgcataacg ttacattcgg ctttggcaca ctccagctgc
     7561 aattcccgtt tggcagtatg atagacatgc cccaccagaa acaaattcag cggcggcacc
     7621 caccagagaa aaaagagggc aacatgccac ggcagtttca cctgtgtgga gtgcagagcg
     7681 atatgcagca gcccctgaaa cactgtgaac cagcagagca gagagaacac acccagatcg
     7741 atcagcagaa ttacccagaa caatttcacg ccccgaaaaa gtgcaccggc agtgagaagt
     7801 tccagacaaa accagacagt gctggtgcgg agcagatcac agccgtgtgc caatgaagcc
     7861 agcttctttt ctcctgctat ggtatgcaca tgggcaaact ggaagaacag gaacaagata
     7921 ccgcacaaga tgccgacaag aattttccaa ccgacagcgg cgtgccatat ggcaaaaatg
     7981 gctgtcagat ttcctgtgac ccaaaatgtc accagccgaa gcaggcgaaa gcctaacgaa
     8041 ttctttttca tctgtattcc tctctgttca gacatttttc atgtcctatt gcaaaaaacc
     8101 ggaccggcag tgctgcctag tccggttttt tctttattca tcaatatggt tttgttccta
     8161 gaaaatcaat catagaaacc atcatctgga gccggaatcg tttcttccgg aacagaaggt
     8221 gccgcagtaa ctactggtgc ctgtgtaaca acaggagcat ttggagccgg cggctgtgtc
     8281 gctgccggag tggtgtatcg gggttttgtt gcgttactgg tattattgtt gtagtatgcc
     8341 cgtgtggtca ctgccggctg atagctgttg tctgtatcgt catcatatga accgtagttg
     8401 ttatacgtat catcatcttc atagactgcc tgtgtgccat aggttttcgc ggtagtaata
     8461 gtttctgtaa tgacacgatt ggcatcggtg gtctttcgct caaatttagt agaaactgct
     8521 gacgctgtgg tttcgctggt gatctgggtt tcttctgtgg aagccacagt taccaccaga
     8581 gaatcattga cgttgttggc ggaactgctt tcctgaatcg gacagagcat caaaaaaatc
     8641 aagatcagca ttgtcaatat cacaaagctg gagcaggtga tcagcgtgat tttggtgggg
     8701 tgtgacaaat ttgcaaagaa gtttttctta ttccaaaacc tttctgaaaa agaaaatttt
     8761 actgttttta cgcaaagaca tttcacttta ttataatgga tttttccgga aaagtcaagc
     8821 ctttctgcaa nnnnnnnnnn agtgaaaata caacaaatga gataattttc atcaaaacat
     8881 tgagataaaa tttgtgcaca agtacaattt taaaaaaaag cttgcttttc tttgtaaaat
     8941 gtgatataat acaaagtgtt gtatcgtcgt tggatttgac gattcttatg cactgatttg
     9001 gagagttatc gaagtggtca taacgagctg cactcgaaat gcagttgtcc gaaagggcac
     9061 gtgggttcga atcccacact ctccgccatg tttttcaaaa agccacgcag tttcgacttt
     9121 gtttgaaatt gtgtggcttt tttagtcttg tttgcaatat tgtgcacgca ttttcgtatt
     9181 ttacggccga gtgctgatat tttgcataaa acatctattt ataaagctta taaagcggga
     9241 aaatcattaa ggcagcttac atttttcaca aattgtgcaa tgaaacaaga aaaaaactgt
     9301 tgactttttc ttgaaaatcg cgtataatga agataatcga ataaaggcat tccgatcaaa
     9361 tcatttcgga aaggaaaggg attaaattaa atgaaacgat tttccaaagg aagcaaacga
     9421 ctgctttccg tcttgctcag cggtgctatg ctggcaggga catgcagttc tgtagccgtg
     9481 ggagctgcag acgaaaaaga agcgaatttt gcacgggcac tgcaatattc catctatttt
     9541 tacgacggca atatgtgcgg cacagaagta caggacaaca accgctacac ttggcgtggc
     9601 aactgccata cctatgatgc tcaggttgcc atgaactcca ccgccaccaa tttaagtgcc
     9661 gatttcctca ccaagtataa agatatactg gatccggacg gggacggcta catcgatgtt
     9721 gccggcggtt tccatgatgc cggcgaccat gtaaaattcg gtatgccgga gaactactct
     9781 gcagcaacac tgggctgggg ctattacgaa ttccgtgatg cttaccagaa aactggtcag
     9841 gacgaccata tcgaaactat tctgcggtac tttaacgact atttgatgaa gtgtaccttc
     9901 ttggattcca acggtgacgt gattgcacat tgctatcagg taggcgacgg tgacattgac
     9961 cacgcttact ggaacgcacc ggaattggat gaaatggatc gtccggcatt tttcctcacc
    10021 ggcgataagc cacagacaga ctatgttgct tctgccgctg cttcgctggc gatcaactac
    10081 ctgaacttca aggacaccga tccggaatat gcacagaagt cgctggacta tgcaacagca
    10141 ctgtataaat tcgcagaaac tcacgaaaag cagctgagcg acaacggcga tggtccgaag
    10201 gcttattaca attcgtccaa gtgggaagac gattactgct gggcttctgc atggatgtac
    10261 aagatcaccg gcgatcacca ctatctggaa cagatcttcc cgtactacga ctactatgca
    10321 gcaccctgct atgtctactg ctggaacgat gtctggggcg gcgtgcagtg tattctgggc
    10381 gagatcacca gcgagcagta cccgaacttc attgacgaat acaagaaagc tgccggaaag
    10441 agtccctacg aggaaatgaa ctgctggagt tccgtagcag aagccctgaa caaatacatg
    10501 actggcggcg tgggaaccat tacaccggct ggttatttct ggctgaacac atggggctct
    10561 gcacgataca atgcggcggc tcagatgatg gcgttggtat acgataaata taacaacaac
    10621 ggaaagcccg gcgaatacag cgaatgggca aagggacaga tggaatatct gctgggggat
    10681 aaccccatga accgtgccta tgaagttggc tacgatgaaa ccgcagcgaa attcccgcac
    10741 caccgtgcag cttctggtct gacaaagtgt gaggacaccg acgagcagaa gcatgtgctc
    10801 tatggtgcac tggtcggcgg cccggatgca caagataagc ataacgacat taccgcagac
    10861 tggatctata acgaagttac cattgactat aacgctgctt ttgtaggagc gtgtgctggt
    10921 ctgtatgatt actacggcac agatgcaatg gagatcacac cggatttccc gccggaagat
    10981 aagaacagcg gcagtgataa cggcggcaac gatttctggg tagatgctta tgcggtagac
    11041 gacatacaga cttccggtgc cggcgtgaca aagcttgcca ttcagatgcg taccaattct
    11101 atcacaccca agaccgacct gtccatgcgg tattatttca gtatcgcaga aatggaaaac
    11161 aaatccaata tcagcaaagt taccggcaat gagttatacg atcaggcaag cgtagaagct
    11221 gctccggcag acggtgtgat ctctggtccg taccagtacg atgcttctta cgatccggat
    11281 atttactatg tagaagtaaa gtgggacggc tataaaattg ccaactccaa caagaaatat
    11341 cagttcaccg tgggcttgta ctacggcgat aagtgggatc ccaccaacga ctggagttat
    11401 cagggcatta ccaagtgcaa ggatacctat caggacggct ccgaaaccag aaccgattac
    11461 atttgtgtat actccaatgg agaactggta ggtggcattg aaccgaatgg caacaagccg
    11521 gcagttacca ccaccacaac acctgcggaa accacgatca ctaccactaa aactactact
    11581 gtaacaacca aagctaccag tacctctgcg gaaactacca caacggagca gacatcatcc
    11641 gctgcacctt ccacagatac aaccgaaacc actgcaaaca ctacaaaaac ctcggaaagc
    11701 ggttctgatg tcacaccatc tgtcatgtac ggtgatgtca atctggacgg tcgaatcgat
    11761 gtgactgatg ccgtgttgct gaataagatg gctgccggtg cagtgacagg gaacgaccag
    11821 cagnnnnnnn nnncggcgtg ccggcgactg caacgttgac ggagaagtgg acaccgcaga
    11881 cagcgttatg cttctgcaat ttttggtaca catcattcaa tacctcggac cgcaggcaaa
    11941 gtaaaggcat tgctgaaacc gaaagaaagg aatatcattt ttcatggagc aagaacatac
    12001 aaaaccaact tccaactggc aggaattctg gcagattctc aggcacggac aattcgggaa
    12061 attatttgcc ggtgccactg aaaatacgct gattcaattc ttccgctact gttttgtagg
    12121 cggagctgca actgtggtag actggggaat ttccgcactg ctgttttttg ccgtatttga
    12181 ccagcattac gccattcttg caaacggctg ttcctttgtt gccggactgc ttgccaacta
    12241 cttcctgagt acattctggg tctttcgttc gtcgaaagtg cagtcccgtc tggtggaatt
    12301 tctcgcattc gccgcaatcg gcgtggtggg acttctgctg acgatcggca ttacaaaggt
    12361 cagcgagata acacttggaa atatcaccag tgcctatcag atgatcggaa aagtagtttc
    12421 cactatggtg gcattcctgt ggaatttctt cgcacggaag tatctcttgt tttcgaaaaa
    12481 agaagcttaa ccggctgaaa aagccgcttt tatccaaaaa aagcactgtc agccgtgggc
    12541 ggttttgccc tgctgtacag tgctttttat atttgaaagg agtgaatcgt ttatgaaaat
    12601 gacatttatc ggggctactc atgaagtcac tggcagctgt acctatctgg aagtctgcgg
    12661 aaagcggctt ctggtggatt tcggtatgga gcaggggaga gatacctatg aaaatgtaaa
    12721 aatcccttgt gctccgtcac aaatcgacta tgtgctgctg acgcatgctc acattgacca
    12781 ctccggcttg ctgccgctgt tgtacgccgg cggcttctcc ggtgagatcc acgctacctc
    12841 agccacgtgt tctctctgtg agatcatgct gcgggacagt gcacacattc aggaattcga
    12901 agcagaatgg cgaaaccgca aggcaatgcg aaaaggcggc gaaccctatg tgccgctgta
    12961 caccatgaaa gaggcattgg gagcactgga actgtttgtg ccacacccat atggcgaacg
    13021 attcacgctg acatccggca ttgatgtgcg gtttctcgat gcgggacatt tgctgggttc
    13081 gtccagcatt gagatctggc tgaccgagaa cggtatttcc aaaaaactgg tattttccgg
    13141 tgacatcggt aacctgaccc agccgctaat tcgtgatccg cagccgccgg aagatgcaga
    13201 ctatgtggtc atggaatcca cctacgggaa ccggctgcac gaaaaaccaa agggatatat
    13261 tccaggcttg gtacaggtgt taaaagatac attcagccgt ggcggtagcg tcatcattcc
    13321 gtcttttgct gtaggacgca cacaggaact actgtactat ttcaagcaga tcaaagatga
    13381 aaacatactg ccggaattca gcgatttccc gatttacatg gacagcccgc ttgccattga
    13441 agcaactgaa attttcatgc gaaataagga aagctgtttc gatgaggaag cactgacata
    13501 tgttcgccgc ggcattaacc ccattggcgt ttccggactg attccggcag tgacaagcga
    13561 tgcttccaaa gcaatcaacg cggacaaacg cccgaaagtc attctgtccg ccagtggtat
    13621 gtgcgaagcc ggacgaatta aacaccactt gaaacacaac ttgtggaagc cggaaaacac
    13681 cattttgttc gttggctatc aggcagaggg ctctttgggt agacgcatta ttgagggagc
    13741 caaaacggta acactgtttg gcgaaaccgt gcaggtcagt gctcagatcg tccagctgca
    13801 gggtatgagt ggacatgcag accaaaaagg gctgctgtcg tggatctcac aagtacaggg
    13861 cgtacagaaa gtgttcgtca accacggttc ggcagaatct gctgaggcat ttcgggaaaa
    13921 attgtggctg tctttgggca ttgatgctta tgcaccgtac agcggtgcgg aatttgacat
    13981 tgccaccgga aaaatcactt acgatgcact tgcggtgccg attcagcaga agccgcgtcg
    14041 gagtgcaatg acatacgacc ggctgtcgca gacgttgagc cgtctgacag cattggtaca
    14101 aaacagcagg ggattgtcca acaaggaact ggcaaaaatg gcagaccagc tgggcgaact
    14161 ctgctccaaa tgggaacgga ggtaatcgaa catgtatgaa gaaaatgcag tactgcatgc
    14221 actttgcacc agaagaagcg tgcggagtta taacagtacc aagccggtgg aacaggaaaa
    14281 aattcaggcg gttctaactg ccggacagta tgctcccaca ggcatgaacc gtatgcctac
    14341 caaatttgtg gtgatacgaa atccggaaat gctgcaccag ctgtccaatc aaaacgcaga
    14401 gatcatgggt gcaacagatc gggatccgtt ttatggagca ccggtggcaa ttctggtact
    14461 ggtagattcc acagtacaca catgggtaga agacggcagc cttgccatgg gtgccatgct
    14521 gaatgcagca tacgctgtcg gtctgggcag ctgctggatt caccgtgcaa gagaaacatt
    14581 tgacagcgaa gccggaaaag cactgctgaa acagtggggg attccggaaa cttatcgcgg
    14641 cgtgggattc tgtattctgg gctacgctgc cggagaactg ccgcagccaa agccgcggaa
    14701 agatgaccag atcctctgga tagactaagg caataccgca tgatgtatag aaaaaggtct
    14761 gatacccata ttgggcacca gacctttttg gataaaaaat gagaatttta ctgcatctta
    14821 taggcgtcga tcatcttttt caccatctgc cctccgatgg atccagcttc ccgtgcagtc
    14881 aggtcgccgt tgtaaccctg cttcagagtt acgccaactt cgctggcaga ttccatctta
    14941 aaacgttcca tagcttcctt gcccttctgc gtaaattcac ccttcatagt gcattctcct
    15001 ttttcatgtc tgggataagc gacttatccc agaagtttgt tttcattttt cagcagctgc
    15061 tttttctcac acgctgtgtt tcgtgttcgc cgctgcaaga atagtatagg acaaaagggc
    15121 gtttctatga caggtagttt tttcgtattt tttctataag cctttgggaa caatggaaaa
    15181 tgctttcttc tgcaaaaaac atatttgcaa aagcaatact gcacaaaata atctatggca
    15241 ataccgcaca aagattgtgc gacagatgcg tcgtcaaatt tttc
//
LOCUS       DAWBGR010000044        15272 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1261_length_15272_cov_4.912006, whole genome shotgun sequence.
ACCESSION   DAWBGR010000044 DAWBGR010000000
VERSION     DAWBGR010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15272)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15272)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15272
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1261_length_15272_cov_4.912006"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3635 a         4320 c         3994 g         3323 t
ORIGIN      
        1 gcacgcagat tgcgtgcaaa gccgtcaggc gggctttatg cagtgtttcc taaatccttt
       61 acagcagaga atgtataaaa agacctgccg aacagtcggc aggcaaatat cacacgaggt
      121 gaatgtttat gaagaaacac aaactcacgg catggctcgc agctgccgca gcggggctgt
      181 ccatgctggg cggcacactc ccgcaggcag ccgtgactgc atctgccgcc ggcaactgcc
      241 agatggaata tctggaccgc ggcatttccg ccatcaacac cggttccggc atgctggtca
      301 gctggcggtt tctcgccaac gattcggaca gtgccgtgtt ccagctgtac cggaacaaca
      361 cgctgatcta caccagcaac gccagagaat ccacctgtta tctggacggc agcggcaaga
      421 gcaccgacca gtatcgggtg gacactgtgg aaaacggcaa agtggtttcc acttccaact
      481 gtaccatgat ctccaatcag aattactttc agatcaatct ggatccgccc accggcagcg
      541 gctgcaccta ctcccccaat gactgttccg tcggcgatgt ggacggggac ggcatgtacg
      601 agattttcat gaaatgggac ccgtccaact ccaaggacaa ttcccagaaa ggcaagacgg
      661 gaaacgtctt tattgactgc taccgactgg acggcacgcg gctgtggcgg attgacctag
      721 gcaagaacat tcgtgccggt gctcactaca cgcagttttt cgtggcagac ttcgactgtg
      781 acggcaaggc agaaatgacc tgcaaaactg ctgacggcac tgtggacggc aagggcaagg
      841 tcatcggcga cgcctccaag gattaccgca actccaacgg ctatgtgctc tccggtccgg
      901 agtactacac cctgttcgac ggcagcaccg gtgcagcact ggacaccgtg aactatgaac
      961 cgtcccgcgg cacagtttcc aagtggggcg acagctacgg caaccgtgtc gaccgcttct
     1021 ggggcactgt ggcatatctg gacggcagca agcccagtgt tgtcaccgga cgaggctatt
     1081 acacccgcat gacagccacg gcgtatgacg tggtcaacaa aaaactggtg aaacgctggg
     1141 cgtttgacac cggaaacgac aagagcgttg ccggctacgg cgacggcaac cacaacagca
     1201 tggctgccga tgtggacggc gacggcaagc aggagatcat caccggctcc acctgtatcg
     1261 acgacaacgg caaggtgctg tggtgtctga acaagggaca cggcgatgcc atgcatctgg
     1321 gcgatcttct ccccgaccgc aaggggctgg aactgtggat ctgccacgaa gacaaaccct
     1381 acggcgtttc gctggtagac gcttccaacg gcaagatcct cttccacaag gacggttccg
     1441 gcgacaccgg acgctgctgt gcagacaatg tctggacagg caacaaggga gcagaattct
     1501 ggggactggg gaacgatgtc tttgacggca gcggcaacac cctcagctgc cgcagaccgg
     1561 cagtcaactt tttgtcctac tgggacggcg atctggaacg ggagattctg gacggctaca
     1621 ccgactcccc tgccaccatt tccaaaatgg gagcaaacgg caagctgacc accctgctca
     1681 ccacagacgg ctattacacc tgcaacacca ccaagggaac gccctgcctg tccgccgaca
     1741 tcttcggcga ctggcgggaa gaactgatcg tccgcggagc agacagcaaa tccattcgca
     1801 tttactgcac gccctacgac acggactacc gcatcacaac tctgatgcat gatccccagt
     1861 atcgggtaca ggtttcggca cagcaaactg cctataacca gccgccccat gccagcttct
     1921 ccctcggctc cgaccagccc ctgccggcac gtcccagcgt cactgtgctg ggacagacta
     1981 ctccaacgcc cacacctacg ccaaaccctg aaccggtaac gcccactgcg gcagttctgc
     2041 cggaaggctc tgtattcatg ctgaaaaatg taaacagcgg actgtatctg gaagttgcca
     2101 acggcagtgc agaagccggt gccaatctcc agcagtgggg tgcggacagt gccgccgccc
     2161 acaacacatg gcgggcgatt tccgccggtg acggttacta ttatctgtac agtcaggtag
     2221 gcgatcagat cacctacctc atggacatct ccggcggcaa ggcagacaac ggcaccaacg
     2281 ctgccctttg gaccaagagc agccgcgaac aggcacaaca gttcctgttc gctctgaacg
     2341 aagacggctc ctacaccatt tacaccaagg cctctaatct gaaaagctgc gtagaggtag
     2401 aaaatgccag caaatccagc ggagcaaatg tccagcagta cgagagcaac ggcaacgcct
     2461 gccagcactg gattctggaa gaaaccaccg ctgccggtgt gaagctggac accagtcagg
     2521 tataccagtt ccagaacgcc aacagtgcac tgtatctgga agtcaaagat gccagtgcag
     2581 aagccggtgc caatgtgcag cagtggagtg ccaacggcaa caactgccag aactggacac
     2641 tgaaagaatt tggtgacggc tactattata tcctgtcccg actgggcgac ggaaagacct
     2701 attatctgga cgttgccgag ggcaagggcg acgacggcac caacattcag atcttcacca
     2761 acaccaaaac ttccgcacag ctgttcaagt ttatccccaa tcccgacggc acctattata
     2821 ttgtgacacg tgcttccaag gataagggag ccttaggcgt tgctgccgcc tccaaggaac
     2881 agggagcaaa cgtgctccag tggaccaaaa acggcagcat ggatcagatg tggaatatta
     2941 ccgctatcgg agccattccc acaccggtga ccactacgga accgccggtg gtgactaccg
     3001 aagcacccac agaaaccact accgctgctc cggtgctgac taccgaagca cccacagaaa
     3061 ctactaccgc ggctccggtg ccgactaccg aagcacccac agaaaccact accgctgctc
     3121 cggtgctgac taccgaagca cccacagaaa ccactaccgc tgctccggtg ccgactaccg
     3181 aagcacccac agaaactact accgcggttc cggtaatgac taccgaagca cccgcagaaa
     3241 ctactactgc ggctccggca accacagtca caacggcagc cacaaaggca actaccaagg
     3301 caaccaccac gaaaaagact tcagaagtac cgagttccac ggttctgtac ggtgacacca
     3361 atctggacgg cagagtggac atcactgatg cagtactgct gaacaaggca gtcgccaatg
     3421 cagttacact gtccagtgct gcccgccaga acagcgactg caggcaggac ggcgaactta
     3481 caagcgatga tgctgtagta ctgatgcagt tcttagtcca tattgtcact aacctgccgc
     3541 agtaaacggc gatagtttaa ggcaataccg cacaaagagc gtctggcgac tttgcacgca
     3601 atctgcgtgc ggtgttgcct tgaacaaaag gatcaacccg tttccggatt tttccggagg
     3661 cgggttgtct tttttgcaag attttccgca ttcccgcata gagtatagag gtacgcagaa
     3721 agtaatcgaa acggaggttt tttctatgtg cggaattacc ggcatggtac atctggaaca
     3781 agacctgcgg cagcaggaac tgctgctgcg gaaaatgcag cgaacactgc aacgccgcgg
     3841 ccccgaccaa gagggcattt ttctctcgcc ccagtgtgct atggcacaca cgcggctggc
     3901 ggtcattgac gtggaaaagg gcaggcagcc tatggaagtc actgccggcg gagaaaccta
     3961 taccattgtc tacaatggcg agctgtacaa caccgccgaa ctgcggacac agctggaaca
     4021 ggagggcaca gtatttcaga cacactgcga cacggaagtg cttctgcaat cctatgtgca
     4081 ctggggtcct cgctgtgtgg aacagtgcaa cggcattttt gcctttgccg tctgggagca
     4141 cagtgcccgc cgactgtttc tggcacggga ccgcatcggc gtaaagccgc tgttttacgc
     4201 ggagatcccc aacggctttg tctttggctc cgagatcaag gcactgctcc agcaccccga
     4261 tgtgccccac gagatcgaca agaccggcat tgcagaactg cttctcatgg ggccggggcg
     4321 gactgccggc ggcggcgtgt tccgcaccat acgggaggtg aagccggcac actgcggcta
     4381 ctacatgcag gggcagctgc atctccaccc ctactgggaa ctgcacgccg cagaacatac
     4441 ggagaacttt gccgacaccg ccgcccatgt gcgggaactg gtgacggatg ccgtccggcg
     4501 gcagatgatc tccgatgtgc ccattggaac attcctgtcc ggcgggctgg attccagcct
     4561 gctctcctcg ctggcaaatc aggtcatgca ggaacagggc agggaactcc acaccttttc
     4621 cgtggactat cgggacaacg accgctactt tcagaaaagc cacttccagc ccaccagcga
     4681 tcccacctat atccgtgcta tggaacgcta tctgggctgc cggcatcact ggacggtgct
     4741 ggacacaccg gctctggcgg aagcactcta tgccgccgta gatgcccgcg acctccccgg
     4801 catggtagac gtggacagtt ccctgctgct gttctgtcgg gagatccggc aggaagtcac
     4861 agtggctctg tccggtgaat gtgccgacga attgttcggc ggatacccgt ggtatcggga
     4921 cgcagacatt cgccgcatca atgggttccc gtgggcacag agtactgcct accggacaca
     4981 gtttctcctg ccggaaattg cggcggagat cgatgcacag gcatacgtgc agagtgccta
     5041 tgcccgcacg gtggctgccg cggaacagct gccggacgat tccccgctgg aatcccgcat
     5101 gcgggaaatg atgaagctga atctggattg gttcatgcag acgctgttag accggaaaga
     5161 ccgcatgagc atgtacaacg ctctggaagt ccgtgtgccg ttctgcgatt accgcattgc
     5221 ggagtatctc tacaatgttc cgtgggagta caaggactgg aacggctacg aaaagggact
     5281 gctgcggacg gcaatgcagg acgttctgcc gccggaagtg ctctggcgga aaaagagtcc
     5341 ctatcccaag acccacaacc ccagctattt gcaggcggtt tcagaaatgc tccggcatgt
     5401 ggtggcagat ccgctttcgc cggtgctgca aatcgtgcgg aaggaaatgc tggaacgact
     5461 gctggaaagc gaggaatccg tgcagtggta cggacagctg atgacacgtc cgcaaattat
     5521 ggcgtacttc gtccagatga actactggct ggaaaaatat caggtaaaag tggtactgta
     5581 agacagcacc cgtcggcatg ccggcggggg ttttttcgtg gctttcactt gacaatcccc
     5641 ggcattgttt gctataatga aaccaatgcg aaaaaatttc gcaaagcaac accgcacaaa
     5701 gcttgtgcgt aagctgcatc gtcagattgc gtgcaaagcc gtcaggcggg ctttgtgcgg
     5761 tgttgccgta attttttgga ggcataaaca atgaaacaaa tcaccagttt tacgatcaat
     5821 cacgactatt tgaagcccgg tatgtacatt tcccgcgtgg acggcgatgt catcacctat
     5881 gatctgcgaa tgaagctccc caacaatccg gcaggcgact atctggagga caacgccctg
     5941 cacacatttg agcacctgtt cgctacctat gtccggaaca ccccgctgtc tgacggcgtg
     6001 gtctatgtgg gacccatggg ctgccgcacc ggcttctatt tcctcacccg caataccgtt
     6061 tccggtgccg atgccattcg gctggtgcag gaaactttac agtatgtact ggacttcacc
     6121 ggcgagatcc ccggcggcaa gcggcaggaa tgcggcaact atctggctca cgatctggag
     6181 ggtgccaagg caatggctcg ggagtatcag cccattctga caaactggac tccgcagcag
     6241 ctgaattatc ccgcagaggt gcttacagga acagcttccg tctgaaaagg aggggttatc
     6301 tatgaccaca tcaaaatatc tgcaaaaaaa cgcagcagaa caggataccg catcaaatac
     6361 aaccctgtcg ccgaacgagc cgacagaatc caacagttct tccttatctg agccaaaacc
     6421 gcccgtggtc actgctgaat aaacgccaga gaacaagccc caaaaacctt tggggaaact
     6481 gctctgggaa agcttctgca tcatacttcc gccagtgctg ctactgactg cggtaggcgt
     6541 ggtggcatac tatattctct gtccgtcaaa aggcgaattt catgcagact gcactgatac
     6601 gctctactgg gcaaaagcga cttatgacag cggaaaactg atcaacccag acttttccta
     6661 tgcttgtctg ctgccatttg gcggcagcct gttgatgttg ttgttcctgc cgcttttcgg
     6721 tttttccatg acaaccaata tgatgggaat gctggtcttt ttccttctgt tcacaacatc
     6781 attgtgttgg atgcttcggc aaatgcactg ggattcccga tggaactgta tcacagcggc
     6841 ggcattcctc atgcttctca gtgccagcaa aaaactgcgg gaaatcttct gggggcatac
     6901 catttattat agtttaggaa tcttattcct gttcttcgga ctggcactgc tgttccggct
     6961 gcaaaatctg tcggcaatac gtcagacgcg agaggtccgc atgcatacga tcctgacctt
     7021 tatcgcactg ttcctgtttt tcatactttg ctgcaccgac cagatcacgg cgatcacgat
     7081 ctttgcactg cctgttctgg ctggtctgtt tctggaacgg gtgctggatc gaaaaacacc
     7141 actgctgtac caaaagaata ctcgtgtact gctgctgttg ctttctctgg gaatcgcaat
     7201 cattgccggc atgaaattgg gaaatctctg ggcaaacggc gttgcaggtg catatgcaga
     7261 taattattcc aactggactg ctcaggaaac ttggacagag cactttcaaa aactgccctt
     7321 ggcatggatg acactgctgg gactggagga cattcccgac aaaaaactaa tgtccggaga
     7381 aagtatcatg aacctgatcc gtatcatcac cgcactgatt ctggcagtgc tgcctgttgc
     7441 agcgacctgc tgttacgcca agtatcgggg acagtccggc agacagatgc gtattttact
     7501 ctgggcacac tggacagtca ccggattgat tctcgtgggc tatctctgcg gtgcactttc
     7561 tgtggcaaac tggcggctca gtccgatcgt ctgcacggct ttcattgtat ctatggcatt
     7621 cctacgctgg gcaattacca aacagaaaac tatgcagcgt gcggcgggcg ttctctgcgt
     7681 gccgctggca tgtttctgcc tgttaagtgc ctgcaatgtc ctgttgctgc ccaccaatgc
     7741 ggaggaagtc aacgtacagt accagctgac caatatgctg gagaaacaag gactgaccta
     7801 tggatatgcc accttctggc atgccaatgc aatcacagtg atctccggcg aaaaactaaa
     7861 agtacgcaat gtcagcgtaa atgagaatgg cgtacaaaag gcgacctatc agactgattc
     7921 taggtggtat gaagatcagc cggaacagaa aaactatttc ctgctgctga acaacgccga
     7981 atatcagaaa cttgacgaaa gaaaagatcc gctgctgaat caggcagaac gttcatttgt
     8041 cctgacagac agcaacggca caacatatca ggtactggtg tttgcaaaaa atattttcta
     8101 aggcaatact gaataaagct tgttccgcag cattccccaa ttctcatgaa attatcacaa
     8161 agtgtgcccg cattttacaa aaagatgtgg tatgctattg tcatcatgaa aagacagccg
     8221 gaatctgttt cccaagattt cggcagattc cccgccgtgt ctttcagcat acggcacaga
     8281 gaggagtatg tatatggaag aatccaaaaa gccaaaaaaa tcatggatct catactggct
     8341 gatcgcgtta tgtgtgttca tgctgcttca gacgtttgtg ttcccgaacc tgttcaaggg
     8401 acgcgtcaag gaagtcggct acgacacatt catcagcatg acagagaaca aggaaatcgg
     8461 ctctgtcaag atcgaaagca gtcagatcac atttactgac aaggacgaaa agcaggtata
     8521 ccagaccggc accctgaacg atccggaact catcgaccgg ctgtacgcct ccggtgcaaa
     8581 gtttgaatcg gagatcgaca gcggcaccag tcctctgctg tacatcctga tcacctgcgt
     8641 gctgcccatt gccctgatgc tgctggtgtt ccacttcttc aacaagaaaa tggtacagcg
     8701 tatgggcggc ggcgacagct ccatgatgtt cggcatgggc aagagcaatg ccaaggtcta
     8761 cgtcaagtcc accaccggta tcaagttcaa ggacgttgcc ggcgaggaag aagccaagga
     8821 actgctggcg gagatcgtgg actatctcca caagccggag cgttacaagg agatcggtgc
     8881 ttccatgccc aagggtgccc tgttggtggg acctcccgga actggtaaaa cactgctggc
     8941 gaaagccgtt gccggcgaag ccaacgtccc gttcttctcc atttccggtt cggaattcgt
     9001 ggaaatgttc gtgggcatgg gtgccgcaaa ggtgcgtgac ctgttcaagc aggcaaacga
     9061 aaaggctccc tgtatcgtct ttatcgatga gatcgatacc attggtaaaa agcgtgacgg
     9121 cggacagtac ggcggcaacg acgaacggga acagaccctg aaccagctgc tgaccgaaat
     9181 ggacggcttc gacggttcca agggcgttgt cattctggcg gcgaccaacc gtccggaatc
     9241 ccttgacccc gccctgctcc gtccgggacg gtttgaccgc cggattccgg tggaactgcc
     9301 ggatctacag ggacgtatcg acattctgaa agtccacgcc cagaaaatca agctggcagg
     9361 aaatgtggat ttctccgcca ttgcccgcac agctgccggt gcttccggtg cagagttggc
     9421 gaacatcatc aacgaggctg cactccgtgc cgttcgtctg ggacgctcct gtgccacaca
     9481 ggaagatctc atggagagcg tggaagtcgt cattgccggc tatcagaaga aaaaccgcgt
     9541 gctgtccaat aaggaaaagc taacggtttc ctaccacgaa gtgggacacg ctctggtggc
     9601 tgccctccag tcccagtctg ctccggtgca gaagatcacc attatccccc gcacttccgg
     9661 tgcactggga tacaccatgc aggtggacga gggcgaacat tacctcatga ccaaaaccga
     9721 aatggaaaac aagatcgcaa cgcttaccgg cggacgtgcc gcagaggaac tgatcttcca
     9781 cgagatcacc accggtgctt ccaacgatat tgaacaggca accaagctgg cacgctctat
     9841 ggtgagccgg ttcggtatga gcagcgagtt tggcatggtg gcaatggaaa ccgtccagag
     9901 ccagtacctc ggcggtgata ccagcctgac ctgctccatg gaaacccaga ccaagatcga
     9961 caagaaggtc acagagatca ttcagacaca gtacgacaag gcaatgcagc tgttacagac
    10021 aaaccagcca aagctgcacg agatcgcgaa gtatctgtat gaacacgaaa cgatcaccgg
    10081 cgaagaattc atgcagattc tcaaccgccc gatgatcgaa caacagtaac tgaaaatttc
    10141 cgaaaagcaa acgccctgca acaattgaac tgttgcaggg cgtttttgtt gcctttattt
    10201 gttgccttta agaaaggtct ggctccggca gtatgttaat cagatgtacc agaaacggca
    10261 tcattgctgc cgacttcgcc gtcactgtcg cagtcggcat ttttctttgc catgctgtcc
    10321 agtgaaacgg ctaaagtcaa ctcatacgca aaaaaagctg tcacatgatt ctttgcatgt
    10381 gacagccttt tataacaaca attgtttacg cttctgtagg tgcagaagaa tctccatttt
    10441 ccgtgggctt ttcttctgtc ggttctgctt ctgttgcaga ggtctccggt tctgccggtg
    10501 tttcctcaaa ctgggaggga tccattttgc ccagcatcag ggtttcgaag tcgtccttct
    10561 cgatcttctc gtgaatcatc agatactttg ccagcagttc cagctgatcc atgtgcattt
    10621 gcaggatcgc cgttgccttg tcgtatgcct tttccacgat gttccgtact tcgtcatcga
    10681 tttcaccggc aacgcggtcg gagtaatcgc gggacgagcc cagatctctg ccgaggaaaa
    10741 cctcgttctg gtcattgcca tagacgatcg gtcccagctt ttcgctgaag ccgtaacgag
    10801 tcaccatgct ccgtgcggta gaagtggcac gttccaggtc gttggaggca ccggtggaga
    10861 tgtcgtccag caccagctgt tctgccacac gtccgcccag cagcgtgatg atgttttctt
    10921 ccatttcctt tttggaaacg aagcttttgt cctgctccgg cagggacatg gtatagccgc
    10981 cagccatacc tctggggata atggacacct gatgcacctt atcctgatga tcgcagtaat
    11041 aggtgcagac tgcatgccct gcctcgtgga atgcggtcag ccgcttttcc ttgggcgtta
    11101 ccttatggga cttcttttcc ggtccggcga tcaccttaat ggtcgcctcc tgaatttcgt
    11161 ccatggtgat tgccttttta tttttccgtg cagcaagcag gcaggcttca ttcaccagat
    11221 tttccagatc agcaccggta aatcccacgg tagacttggc aatggtagtc aggtctacgt
    11281 ccggtccgat gctcttgctc ttggtgtgta ctttcaggat ctgttcccga cccttaacgt
    11341 ccggatagcc aactacgatc tgacggtcaa accgtcccgg acgcagcaga gccggatcca
    11401 gaatatcccg ccggttggtg gcagcaatga caatgacgtt gtccttgccg gtaaagccgt
    11461 ccatttccac cagcagctgg ttcagggtct gttcccgttc gtcgtgtccg ccgccgatgc
    11521 cggtgccgcg gtggcggccg actgcgtcaa tttcatcgat aaagataata gacggagcgt
    11581 ttttcttcgc ctgatcgaac aggtcacgga cacgggacgc accgacaccc acaaacattt
    11641 ccacgaaatc cgaaccggaa atggagaaga acgggcagcc ggcttctcct gcaacagctc
    11701 tcgccagcag agtcttaccg gttccgggag gtcccatgag cagtacgccc ttggggatac
    11761 gggcacccag ttccgcatac ttccgcggat ttttcaggaa gtctacaatt tcttccagtt
    11821 catgcttttc ctcatcggca cctgccacat cggcaaaggt tgccttgcgg gtgttcgcct
    11881 gattcttgat gtttgccttg ccgaaggtgg tgtacttgcc gccgccgttt gcctgccgca
    11941 tcatgaacac gaacaggaag atcatcagcc ccagcagcac cagtgtggga atcagcgtca
    12001 gcagccacga agtatcccga atcttataat agtccacctg cagcggagca tcggggtgct
    12061 tctggttata gctggtgcga tagtccttcg tatcatcacg gaataccgat acattgggca
    12121 catcataggt cagcttatcc ccgtcgtcca gcgtcagctt cagttcgccg gagcccagat
    12181 ccaaggtgta cgcagtaact tcataattat cgaagtactt cataatgtcg gaatatttgg
    12241 tttctttgcc gccggttttc agcccgttca gcaggaaaac cagtgccacg atcaatacca
    12301 cgacaacgag cagatacgct ccgatcccac ggcctttctt tggtgtcaaa ttacattcac
    12361 tccattctgt tgaaaagaaa accggcaggc agcattgccg ccagttctct ctatatcatt
    12421 gcgagggaac tgctgcatca ggtttacgca caagcttcag catttcccga gaatttccag
    12481 caccagcagc cgtctggttt ctccgtccgg acaaactctt gcggcacagc ccacgccctc
    12541 ggcgaaaatt actccgtctg catctgccag cacatgagcc gtgcggcggc gttccggcgg
    12601 aagattcgcc tgtatcagtt ttttcagagc agaggtgaac cctctgccgg aaagctgcat
    12661 gcggtcgccg gactgtctgg gacgcaggaa gatttcacct tttattttat cataatccag
    12721 acacgcattt gcaaattttt tatgaatatt tgcaacattt tccgcaggcg acactgtttc
    12781 cagtcgggca tgcaacacaa aacctgaaaa aagccggttt tcccccattt gcagagggat
    12841 cgcttccggc aaaggctccg gcagctgcac cgtttctatg gtcagtgtgc ccgactttcc
    12901 cacagcataa acagcaccag acaaattcca cctgccgccc tgccgtgcca gtgccgccag
    12961 ccgttccagc agcggagctt cacaggagat ccccgcctcc tccagcagca tggcaataca
    13021 ccggaactgc aacgcctcgt gaagcgtttc caagccggaa agggtattct ttccggtatg
    13081 gcatttggca taggctgcct gtgcctgctg gtgcaggaaa tcgtcctccc gccgcaggct
    13141 ccgcagggta taggaaaccg tgcgttctac agaagggttc agctgttcca gtgccggaat
    13201 cacctgatgc cggatccggt tgcgggtgta ggcatcggtg aggttggtgc tgtcggtaac
    13261 ataggtctgc cccagtgatt ccagatactg ttccacctgc cgccgtgtca cccgcagcag
    13321 tggacggaga aaccgcccgt ttttcggcgg aattgccgtc agtccatgca ggcttgctcc
    13381 ccgcaccagc cggtgcactg ccgtttccag attgtccgag gcagtgtgtg ccgtggcgat
    13441 ccagtccgcc tgcacctgtg caaaggcagc ataccggcat tcccttgccg ccagttcctc
    13501 ggagcagtgc tcctgtgctg cccttgctgc cggattcaca tcatagaccg ccagccgcac
    13561 gcccagcccc gcacaaagct gctgacagaa tgttctgtcc cgttcgcttt cggctccgcg
    13621 gagatggtga ttgacatgca ccgctgtaat tgtaataccc agtaccggac gcagttccag
    13681 caggcagcgg agcagacaca cgctgtccgc cccgccggaa agggcacagc agacagtttg
    13741 ttccggttcc agacagtgca gctcccgcag ttgctccagt actgcacggg aaacagcatc
    13801 agccctcatc acgcatttcc gcattggaga agcgagtgaa ctgaccgtcc cacatcagct
    13861 taacggtacc ggtttcgccg tgacggttct ttgccacgat gcactcggaa atgttcggcg
    13921 tggggctgtc cttattatag taagcatccc gatagagaaa cattacaatg tccgcgtcct
    13981 gctcgataga accggattca cggaggtcgg acagcatcgg ccgcttgtcg ttacgtcctt
    14041 ccggaccacg ggacagctgg gacagaacaa taatgggaat atccagttct ttcgccatga
    14101 ttttcagctg acgcgtcagt tccgaaacca ccagtacacg gttgtcggtt ttcagggtag
    14161 aacccatcag ctggagatag tcaatgacca ccagtcccag attcttcata cgccgcagct
    14221 tcgccttgat ctgctgcacg ttgatgctgg cagtatcgtc aatgtacatg ggcagtccgc
    14281 taagaacgcc ggcactggtg gcaagccgca cccagtcctc gttggtgaga aagccgttgc
    14341 gaagcttctg ggaatctacc agtgcttccg aagaaagcat acgggtggca agctgctcct
    14401 tggacatttc cagcgaaaag atcgccactt ccttgccgct gtgccgtgcc acgttggtgg
    14461 caatgttcag ggcgaaagaa gttttaccca taccggggcg ggcagcgatc agcagcagat
    14521 ccgacttgtt cagaccggaa gtcaccgtat ccagatagga aaatcctgtt ctggcaccta
    14581 catatttttc tttgtccggc cctgcaattt tgcccacatg gtcataggct tcacaaatgg
    14641 catcgccgat cttggtcagc ccctgcacgt ccttgccctg ccgaatgtca taaatccgct
    14701 gttctgccgc gtccatgagc atctgggcac tgccctcgcc ggactgaatg tcctgcaaaa
    14761 tgccccgtgc cgcatatgcc agacaacgga tataatattt ttccgtgaca atggcacagt
    14821 agctctcgat attcgaggta gacggcacac tgttgattaa cgccgccaga tagtttctgc
    14881 cctctgcggc ggtttcgaac accttgcgtt gcactgccac attgagcacc gtcaccacgt
    14941 ctgctctttc accggaagtg aacatttgca gaatgatctg gaacagttcc tgatgctggg
    15001 gattgaaaaa gcattccgga cgaacggttt ccaatactac cggcagtaca ttcgggtctt
    15061 gcagaatggc acccagcacg gtctgttctg cttccagact aaagggcagt tctgcggctt
    15121 cgttggaaga atacactgta tctgccatga aaatccctcc ttggaaaaat aatgcgggac
    15181 gaaaaccgta cccgcattgc tctgttgctg atgattctgg gcaataccgc acaaagattg
    15241 tgcgacagat gcgtcgtcaa atttttcgtc ag
//
LOCUS       DAWBGR010000045        14920 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1285_length_14920_cov_4.483687, whole genome shotgun sequence.
ACCESSION   DAWBGR010000045 DAWBGR010000000
VERSION     DAWBGR010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14920)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14920)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14920
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1285_length_14920_cov_4.483687"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3186 a         4308 c         3689 g         3737 t
ORIGIN      
        1 tttgtttcat ctgacgaaaa atttgacgac gcatctgtcg cacaatcttt gtgctgtatt
       61 gcccttactt ctcaacagct tcctgctccg gcatattcgc cggctttgcc ggatctccga
      121 caatgaccgg ttccagttcc agctgatagc cggttttttc cagaacggtc tgctttacaa
      181 aatcgcacag ttccagcaca tcgcgacaag ttgccttgcc ggtattgacc acgaagccgg
      241 catgctttac gctcacctgt gcatcgccga cgctggcacc tttcagaccg cactggtcga
      301 tcagaaatga ggcataattg cccactggtc tttgaaaggt gctgccggca ctgggcagct
      361 ccagcggctg ttttgcccgc cgctttccca gcagagagtc catttcctct ttgattgccc
      421 catagctgcc cttttgcagc tgaagcgtga cacccagaat gattctgccg tcctgccgga
      481 aaatactttt ccggtagccc agttccattt cagattttgg taccacagag atcgtgccgt
      541 ccggtgccat acagcgtgcg gaaactatca cgtctgccat ctcgccgccg tatgcccctg
      601 cgttcatgta cagggcaccg cccacagtcc ccggaatgcc ggcaaggcac tccatgccgg
      661 tcagagattc ttccagagcg aacttgccga tacggcccag tgtggctccg gcttcacagt
      721 acagcgtggt gccgtcctgc cgaatctggg aaaagtcttt tcccagatgc aggatcacgc
      781 cggcatagcc gctgtctgcc gccagaatat tgctgccgtt tcccaatacc agatagggat
      841 agttccggtc acggcacaga ccgagtatct gccgcagtgt ttcttcggaa ttcacgttca
      901 cgaaaacact acaggctccg ccgatccgga acgtggtatg gttccgaagc ggctctgcat
      961 actgcacgct gcaattgcac gtcctgcaaa tcgcagcaag ttcctgccaa tttttcatta
     1021 ttttctccta aaagggattc tgatttcgtt ttgccgactg tccgccggtt aaaaatcatg
     1081 ctgttgctct taggcaatac cgcacaaaga gcgtctggcg actttgcacg ccatctgctt
     1141 gcaaattttc cgtcagagcc tgtttcgtat cttctcgcac gcatctgtcg cacaatcttt
     1201 gtgcggtatt gcccttaaaa tgcgtcgtaa tccttgacaa cttccttgga ttccatatcc
     1261 agtcccagct gatccagcag ttcggttgcc gcattgtagc ccattttttt ctgacggttg
     1321 ttcattgcag caacttccag aatgactgcc agatttctgc ccggctttac cggaatggtc
     1381 agcatcggga tctttacgcc cagaatggtg gcaaactggg tatccacacc catacggtcg
     1441 tagatctttt ccggattcca ctgttccatt tccacgatca gctcaatttt ttccgtgttc
     1501 tttaccgcac cgataccgaa cagccggcgg gcgttgatga taccgatacc acgcagttcc
     1561 aggaagtggc gaatattgtc cggagaactg cccaccagac tgatgttgga aaccttgcgg
     1621 atctctaccg catcatctgc caccagacgg tgaccacgct tgacaagctc aattgcagtt
     1681 tcgctcttgc cgacaccgct ttcgccggta atgaatacgc cttcgccgta gatctcgatc
     1741 agaacgccgt ggcgtgtgat gcggggagcc agattcaggt tcaggaatgc gatcagaccg
     1801 gagataaagt tggaagtgct ctccttactg cggagcagcg gcacctcgta ctttttcgcc
     1861 agatccagca tttccgaaaa atacggcagt tcccgtgcaa taatcagtgc cggaatgtgg
     1921 cgggacatca gagcgtccag acgctgggtg cgggtttctt cgtcgatcgt ggagagatat
     1981 gcaaactcca ttttaccgat gatctgcata cgttccggat tgaagtactc atagaagccc
     2041 atgagctgca atcccggacg agtgacatcg ttttcgtcga tcaggatctc tgtggggtct
     2101 ttgggcatat aaatggtttc cagatgaaat tcatcaatga tttttcctaa tgatacagta
     2161 aattttccag ccattctgac cagctccttt atatttattc tgtctgttat ttttcagtca
     2221 gcacaaccgc agaaacgccg ttgtctgctg cacttctcaa tgtttgtaat tcctcggtag
     2281 aggctccgct gatctcagca gtcagacaca gcggcatctc cgccgtcttt gcctgaatct
     2341 gcggcagtat tgcctgtgcc tgccctgcat ctgcctgaag atccagcacc acgccggaaa
     2401 ctgtcaggtt ctcaggatca aatacttccg ctgttccggc ggcagcatct gcggcagaag
     2461 tttccaccca cagcggcaca ctgccggcag tctgtgccag cgtgttcacc aaaccggtca
     2521 gagccgtttt ccgttccgga ttctgtacgg attctccgat ataggtgagg tcggagttcc
     2581 ggaaatcggg aaacaccacg tccgtgcacc agatttgaga gaagccggct gcggtcacct
     2641 cggcggtaat gtctttcagg tactgcactg taacatcgct gaacggactc gcccacgcct
     2701 tgccgccgtt ttccttggta ttgtccagcc agcgtccgcc gctggtcacc atataccctg
     2761 cctctgcatc tgcgtccggc agcaggttat cccgcagcag gctcagctgt gccaccggtg
     2821 tccagccctg tgcctttgcc gcttctgcga tctccgccag ctgcaggctg ccctttgccg
     2881 caccgcagct tttcgccagt gccacatctg tctgatagca cagttccccg ccggcaattt
     2941 tcagcgggat caccagcagt tttcccgccg gcactgcatt tcgtgcagtc tgcatggctg
     3001 cggaaagtgt gctgctgttt tccagtgccg ccgcagaaag ctgatagccc tcggacagcg
     3061 attcttctgc tacagccggt gtggaagtgg caactgccgg cagtgtttct gcctcggtgg
     3121 agatctccgc tgttgccgct gcggtggctg ccggtaaagt tccctccgcc tgctccgtga
     3181 cacggggagc accggagtat tccagaatcg gcttggcaat gctgtacccc accacgccga
     3241 tcacgccggc aaccagaaag gtaaagccga ccgataaaat cgctctggcg ggctgcactt
     3301 ttcgccgccg ctttttggaa taggtatgca ctttcggctg acgtttggat tgcaacttca
     3361 ttcctccttg gagcgttttt aatgtgcctt aaggcaaccc cgcacaaagc ttgtgcgtaa
     3421 tgcggggttg ccttaaatat tatttatgct ctgccctgtg ccgatagacc ggcagcttct
     3481 tcccgaatgg cattcatcgc cgccttggga tctgcctgcc ggaacaggta actgccgatg
     3541 accagaattt ccgcaccggc ttcaatgaca cgaggagccg tttcggcgtt gataccgccg
     3601 tctacttcga tctggtaaga cagcccgtgt tcctgtgcat actgccgcag tgcctttacc
     3661 ttttccacca tgtcccacag gaacgactgc ccgccgaatc cgggttccac agtcatgaca
     3721 agcaccactt ctacctgctc cagataggga atggcttctt cccacggggt gttgggtttc
     3781 aggacaatgc ctgccttaac gccggcttca tgaatggcag caatcgtcgc cgccggatcg
     3841 ctgttgcttt cgatatgaaa cgtgatcaga tctgcaccgg cttctgcaaa ctgctttaca
     3901 tagcggatcg gatcggcgat catcaggtgt acatctaaaa acaggttggt gcagtggtct
     3961 actgccgcca gaaccggcat accaaatgta atattgggca caaactgccc gtccatcacg
     4021 tcaaaatgca gcatgtccag accgtcgcgt tccagtccct ctgtgactgc cgtcaggttt
     4081 gccatatctg cactcagaat tgacgcagaa atttgaattg ttttcaaaag cgtcatgctc
     4141 ctctcccgaa ctttgcagtt ctgcttctct gctgcgtgcc gaaggttcac agcaaaatgc
     4201 agaatgcttc ttattttata ttatataata aatccggata ttcgtcaata gcgtttttct
     4261 gagaacatgt gacttttttg ccggcagctt cagcgggaat tttcgcaaaa cttgacaaaa
     4321 gtacggaaat ccggtataat aaagcatgtt tatatacggc aacaccgcac aaaacccgcc
     4381 tgacggcttt acacgcaatc tgcttgcagt gttgccttta ggcaataccg cacaaagagc
     4441 gtctggcgac tttgccttta tttttttctg aaagaggtgt aaccagtttc atgatcggaa
     4501 tcgcactgga gggcggtgca gaacgcagtt cctttaccgc cggtgttatc gatgctctga
     4561 tggcacgtga tttctacgcc gctgctgcgt ccggcacgtc tgccggtgcc ggctgtgtgc
     4621 tgaattatcg ttcccgtcaa cagggcgttt cactggaaat gatgatcatg gaacggcaca
     4681 gccgctactt cgggatccgc catatggcac acaccggcag attcctcaat ctggatcaca
     4741 tggcacagga gtacgccagc cgcatcgact ggactgttta ccagcagttc gccatgcaga
     4801 ccgattttgt ggcgacctgc tgcgaggacg gcgaaccggc atacctcact gatgacggca
     4861 gcaggcaccg gctgtttacc gcgatcaagg cttcctgcgc cctgcccatt atctgcgaac
     4921 cggtggaact ggacggcaaa cactatgtag acggcagcat tgcagacccg attccctttc
     4981 tgcatctgct ggagcagggc tgtgacaagg tgatcgtcgt tctcaccggc agcgtgcatg
     5041 cacgtcccac agattacacc cgcctgcgtc ctctgctgcg gcagctgtat gccaaaaaat
     5101 accccatgct gtaccacgcc gttctccacc gcatcgcccg ctatgaagaa caggtacatg
     5161 ctctggagca ggcacagcgg gaacagcggg cactggtgct tcgaccacag gtcaagtcga
     5221 tcccgctgtt cacccagaat gaggacaaga tccgtgccta ctaccagcac ggctgcgaac
     5281 tggtagagca gcggtggacg gagatcacgg cgtttctgga aagccggaca ctaacacaat
     5341 aatatgaaaa ccacctgtat gagagagatc ttgtacaggt ggttttttta atactcgatg
     5401 ccctttcgtg ctgatatgcc ttcttcatac gggtgcttcc gcagacacat ttcggtgagg
     5461 tagtccgccc gttctgccag tgcaggactg ggatttctgc cggtcatgac gacttccaga
     5521 ttctccggac ggctgtccag aaatgccagc accgtttcct ccggcaaaaa cccacaggag
     5581 atacaggaga gcaattcgtc cagtatcagc atgcgatacg tcccgccctg cactgcctcg
     5641 gtaattctgc ggaattgtcc ggtataccag tgagccgcct ctgccttttc cgccggtgtc
     5701 atgcgaaagg aaaacttggc atcgggataa gtttcccaca aggtgacctg cggcagctgc
     5761 tccagaatcc gccgttctcc ggagttatcc tgcttcatca gctgcaccac cagcacccgt
     5821 tcctgccaac cggcacagcg taccgcaagc cccattgccg cagtcgtctt acccttgccg
     5881 tcgccgcagt agagatgtac cagaccgctc atttcgtctg caccgcccag tccggcagca
     5941 gcacttcccg attggtgccg taaaaaatat cgtccctgcc ggcaagcatc tcacaaaggc
     6001 gttccagtct gtcccagttg cggttgcctt cgaattcata gctgtgtccc cagatgtaga
     6061 agatctgggg cgtttcagct tctgcctgca aaaactgttc cgccagtgag aacagagctt
     6121 cgtcatcgtg gtgacaggtg ggacgaaacc gcagcaagtc ctcctgttcc gcgaagcagt
     6181 gagagctttc cgtgcctcgt ccgtagccga ttccaagccg ccgcagttct tccacaaccg
     6241 catcattgaa cacaccgtag ggatatgcca tacccaccgg aacagtgccg taaatctctg
     6301 tgattgccgc aatattgtcc tgcaattccg ctgtgcactc ctccggtgtc agttccgtca
     6361 gattgtagtg gtaaacaccg tggacagcca tttcatgacc ggcatagagt tccggacact
     6421 ctgccagatt cagccggtgc acttccagca cattccggta tacccatgtg ccgcagtcgt
     6481 ggttcagtcc ggtgttgacg ttaaacgtgg ctttcatgcc atagcggttc aggatctcca
     6541 gcagctttct gtcctgctcg ttgccgtcgt catagctgaa tgtcagggct tttcgatagt
     6601 gatttttcca gttcattggg gaactccttt ccttgtgggt agtcactttt ggcattatac
     6661 catttgcagg cggatttgtc aattccaagc gtgtttcgaa aaatactggc acccgttccg
     6721 gaacagagag cagcatcttc ccgcagcttt cgaaaaaaga cgaacttcca cgagttttcc
     6781 cgaacatatt gcaggcggat tcataatatg ctatattaga gtgtgctgat attttgacac
     6841 gatcaggaaa cactacataa ttttccaaag gaggtatgtc ccctgcactc tatccagaaa
     6901 aagcggctgg ctgctgccat gctcacctgt gctgttctgc atcagcagtc tatgccgcag
     6961 ccgcctccga ttcctgtaca gaacaatgcg aagccagact tgacactgca ctttctgcac
     7021 gcaccggcaa tttctcagct gcactgtcac acgataaaat aagaaagccg tcctcagggt
     7081 gcatcccgag agcggctttc tgtgtgcaac tgaatatatg aaatccgttt cgccctcacg
     7141 aggagaacaa cacgggattc accttaatag agcggaccgt agtcggcaca cgcacgatag
     7201 tccgcagcct ccagatcctt ggtgccatag gcactccatg tgtgcggacg atagatccgg
     7261 tcttcggaaa cattgtgcat cgaaaccgga atccgcagca tgcttgccag cgtgatcagg
     7321 tcggctccga tatgcccgta tgccagacag ccgtggtttg caccccagtt tgccatgaca
     7381 tggtacacgt ctgtaaacgc accctctccc gtcagattcg gcacgaacca tgtggtcggc
     7441 catgtcttat cggtacgctc gtccaaaggc tgatgaatct cgtccggcag gtctactgta
     7501 tagccctctg cgatttgcag cagcggctgt gtgccgttga gaatgttcac gcgaatcatc
     7561 gtcaccggca tctctgcacg ggtccgatag tgcgaggaga agccgccgcc gcggaaatag
     7621 cccagatttg ccggtgccca gtcggtcgct gccagcattg cctgaatatc atcggtgctg
     7681 acttcccacc acggcttcat gaccgccgtg ccattttcgt ccctgcatgc accggtgccg
     7741 tccagagccg ctgcaccgct gttgatcaag tggataaagc cgccggctgc ccgtccggta
     7801 ggtgcgacac ctgtcacacg ctttaccgct tccggactcc agtaggttcg aacatcggca
     7861 aagatcgttg ccgtgccggt gagcagtttg ccgaacagca tcgccgtacc attcaggccg
     7921 tcattttcgg ttgccagaat ggacggctct tttttcccgt tccagtcaaa gctggaattg
     7981 agaatggctt cggtgaagtc gccgttgggg agccagtctg tccacatacg ctgtccctga
     8041 aagccgccca gaattgcatt tctgccgtgg ctttcttctg cccagcccat ttccagcagc
     8101 ttcggattgc cgaagaaaat gtcctgtacg atcagcgtaa acttaacaat gaattcccat
     8161 tcctttgcct tttcttcggc agtgcggggc ttcttgatgc cgaaggggtc gatttcctta
     8221 tcctccgaca cgtcccgatt gacatcatag ccctccggac aattcgcctt tacccacgcc
     8281 agagcctttt catactcgtc gtggtcatag atgcccagat ggatccggcg gagaatttca
     8341 gtcaggtcta cccattctgc acggattccc agatacttct ggaaaaagtc tgaattgcag
     8401 taggagccgg cgatccccat ggacacgccg cccagaccga catacgcctt attccgcatg
     8461 acacccaccg caacggcaca cttggcaaac cgcaggatct tttcctctac atctgccgga
     8521 atcggcatgt aggctttctt ttcgggaatc gctccggatt ccggaacgtc agactgccac
     8581 ttgtcctgca cgtctttgcc gtaaatggca aacgccggca agccacgctg tgcatgagcc
     8641 gccatgcagg ctgccagata aacggctccc ggacgctcgg tgccattgaa tccccacact
     8701 gcctttatgg tcagcgggtt cagatccatg gtttcgctgc cgtagcacca gcagggcgtt
     8761 acggacaggg tagcacacac gttctgtgtc tgaaaaaagg tttcacattc ataggcttct
     8821 gccccgccgc caatggtttt cggggcgatg acgcactgca cctttgtgcc gtccggatac
     8881 cgcagacggg actcgatcag cttctgtgct gcatatgcca tgtccatggt ctgctgttcc
     8941 agatcttcac ggacaccgaa ctgtctgccg tcaatgaccg gacggatacc gattttcgga
     9001 tatatcataa actcaacctc acttcaacag ggttcgatat ttctcataag atgacgcagg
     9061 gaatgctcct gcatgataaa actgcatcga ttccgatgcg gcaatgcaac gccgtgcttc
     9121 tgccggcgtt ttcaaaattc ccagtgccag catttgtatc gcggcatttc cgtacacggt
     9181 agcttctacc ggtccggctg ccaccagccg ctgacaataa tccgctgtca gctgacagag
     9241 cagactgtcc ttgacaccgc cgcccatgat gtggatcaca ggataggttt caccggtgca
     9301 ggcttccagt tcttccagag ccatgcggta tttcagtgcc agactttcat agatgcagcg
     9361 gaaaatggct ccgtcgctct ccggcacatg ctgaccggtt ttccggcaaa acgccttcac
     9421 ccgcttggga atatccccgt ggggcacaaa gaccggatcg tccggatcga taaagctgga
     9481 cggttcgtgg ctctgccgtg ccatggtttc gatctccgca aagctgtagt tctttccctg
     9541 acgggcatat tcccgacggg actcctgcaa cagccaaaga ccgatgatgt tcttgaggaa
     9601 cgtcaccctt ttcccatagc cgacctcatt ggacaagccc agtgccgccg cctgctctgt
     9661 cagaaccggc tgttccggct cggttccgaa cagggaccat gtgccgcagc tcaggaacac
     9721 atgcttgtct gtctttgccg gagccgcaaa tgcagcacac tgggtatcgt gcccgcagac
     9781 cgccgtcacg catatcgccg gcaagcccag ccgctgctgt acctccggag aaagcgtgcc
     9841 ttttgccgtg ccgctttcca caatttccgg cagaatccgg tggggaatgt ggaaaagctc
     9901 cagcagttcc cgatcccatg tgccggtttc cgcggagagc atctgagtag tgctggcgat
     9961 agagcgttct gcggaaatgg ttcccgtcag cagatacgca aacagatccg gcattggcag
    10021 aatcgtggca cactggtcaa agccgccgct gtcccgctga cagagcagct gaaacagcgt
    10081 gttgatttcc atgacctgtg tgcctgtccg gcggtagagt tccgctgccg gcaggaactg
    10141 ttctgccttt ttcaaaatac cgtctgttct gccgtcccga taatgccgcg gcagttccag
    10201 caaggtgccg tctgccccca gcataccgta gtccacgccc cacgtatcaa tggcgatgct
    10261 ttcatagggt gcaatggcat agcccgcctg caagccctgt tccagttctt ccatgagctg
    10321 cggcacgttc cagtacaatc tgccgtcctt ggaaacgggt tcattggcaa agcggtgcac
    10381 ttcctgcaag gtgatgcggc tgccgtcaaa acctgccagc atggcacggc cggaggacgc
    10441 tccgaaatcc agtgccagca cataccgcat catggggttt cctccggcag gactacgccc
    10501 tttttcaaca ggacgttggc gtagatcgcc gtttccccgg tggcaattac ggcgtatgct
    10561 tttttggcac gctcgtaaaa tgcgaaccgt tccataaatt cgatctcaca gtgctccggt
    10621 tcgtaccggt tcaggatctc acggtaggtg tcccaaatta caggaactgc cggatcgccg
    10681 gctaccactt ccataagtcc caccggcttt tccgtgtagc tgtccagcgg aaagaactgc
    10741 atcaccgcgt ccagaatctc cggcacgccg tgaccgtcca gacggatcag ccgctgtgca
    10801 attgccgcac cggggaaatt gccgtcggcg atcacaattt catcgccgtg tcccatttcc
    10861 atgagtactt tcaaaagctc cggtgtcaga atcgccggaa tgtgtttcag cataaaaaac
    10921 acctccgatt cagttctgac cgttggggta cttcttatag ccggggtgct ttcccgttac
    10981 gccgtactgt gcacgcatgc tgcaaagccg gtcgatgttc tcccgcgaga tctcctgtgc
    11041 accgcccagc aggtgggcat tcagggagat ctttgcaaag agttccagcg tttccatgcg
    11101 gtagtacgca gtgatcaggt ctgctcccac cgtcagggca ccatgatttt gcagaagcat
    11161 cacgtcatgc tcctgcaaat agggcttgat cgcctccggc acttcgtctg tagagggcgt
    11221 gccgtaaggt gtcaccggca cagagccaat ggcaattacc gactcgatca tggtgtactc
    11281 gtccagtgcc ttgttggcaa cggcataacc ggttgccaca ggcgggtgtg catgcagcac
    11341 agctcccaca tcgggacgtt cccgatagca gcagaggtgc attttgattt ccgaggacgg
    11401 ccgcagccct gcagccccct ccagcacttc gcccttgtcg ttgatgcgaa ccagcttgtc
    11461 cggtgtgatg aagcttttgc tcatgccggt aggtgttgca aaaaaggtgc cgtcctccag
    11521 cttcacggaa acatttccgt cattggcagc cacccagccc agctgccaca tcttgtggca
    11581 gacatcacaa agctgttctt tgatttcctg ttcgttcatt gaatcctcct gtaagaacca
    11641 catgggttca tctccttgtc agaccattct gcaaatctga cgttctgacg atacgatacc
    11701 gtccgacaga aattccgcga tagttccaga ccgttccgca taagctgcga aaaaatgccc
    11761 gcttggaagg catctcggaa aatcccatct ttattataca attttctgat ggaattgtcc
    11821 agctttttcg gctgaaattt acattttatt tggtgaacaa tttatttccc gcactgccgg
    11881 aacgggtttc tccgcagaat tccgtcatat ttcatcggac agatcacaac aaaaaagcac
    11941 accgaagccg tatgtgctcc ggtatgctct gctctttcaa aatgcttttc gcatggtcac
    12001 atggggacaa aactcgtcca tgtgatactc ttctgttttg gtatagccca gcttttcata
    12061 aaagcctgcc gcacggcatt gtgccgaaag ggtaatgcac ttggcaccgc atgccttcgc
    12121 ctgctgttcc agtgcagtga caacgtgttc tcccagatgc tgcccccgat aggcagacag
    12181 cacggcaact ctgccgatga gatatgtttc gccgtcctcc gtgaacagtc gtccggtggc
    12241 aacgggacgc tctccgtcct gtatcagcac gtgatacgca cggtcgtcca cgtcgtcgaa
    12301 ctcctgctga aacccctgtt cttccatgaa aacggctttt cgcacagcgg cacagccctc
    12361 gttaagctgc ttcaaaatga cagtctgata cgccattgct cacagtacct tggacaggaa
    12421 ctctcgcagg cggggattct tggggttgct gaaaaattcc tgcggttcgt tttcttccac
    12481 gatctttcca ccgtccatga acatcacacg ggaggcaact tccttggcaa agcccatttc
    12541 gtgagtcacc acaatcatgg tcatgccgtc gtctgccagt tcccgcatca gttccagtac
    12601 ttcgccgacc atttccggat ccagtgcgga agtcggttcg tcaaacagca tcacatccgg
    12661 attcattgcc agtgctctgg caatggcgat acgctgtttc tgtccgccag aaagctggtc
    12721 gggatactgg tttgccttgt ctgccagtcc gactttttgc agcagttctg tggctttggc
    12781 atctgcctcc gcctgtgtca tgcgtttcag ctttaccggt gcaagagtga tattcttctg
    12841 aatggtcagg tgcgggaaca ggtgaaaatg ctggaaaacc atgcccattt tctgccgcac
    12901 gctgtaaagc tgtcggtcgg gcatgtgcgt caggtcttca ccctcaaaga agatctgtcc
    12961 gtcgctgggc tgttccagac ggttcagaca gcggagaaag gtgctcttgc cggaaccgga
    13021 cggcccgatg atgactacct tttcgccctt ttcgatgttt gtggaaatgt ctttcagaat
    13081 atggagatct ccaaaggatt tgtccagatg tttaacctcg atcacttctc gccaatctcc
    13141 tttccagttt tgtcaccaga gaactcaaga tcatgaccat gatcagatag atcagggcaa
    13201 ctgcgatcag cggcaggaat gcctcgtagg tctggcttcg gataatatcg ccgcccttgg
    13261 tcaggtcgat caccgcaata tagccggcaa ccgaagtttc tttcagcaga acgatgcact
    13321 cgttcgccag tgccggcagc acgttcttga acgcctgtgg cagaatgacg gagagcagtg
    13381 ttttcgggta gttcagtccc agactggcac ttgcctcaaa ctgtccgttg tcgatcgaca
    13441 taatgccgga acgcacgatc tctgccacat acgccgcaga gttcagaccg aacgccagca
    13501 ccgccacaaa ggttttcccc acattcaccg aagcgaaaat gacaaagtag ataatcaaca
    13561 gctgtacaac gacaggcgtg ccgcggatca ctgtcagata gatccgtgcc agaacattca
    13621 gaaagcccag ctttccggtc ttgtcgtggg tggaacggac aatggcaatg agaaagccca
    13681 gcaccatgcc caggatcacc gcgaaaaatg tgatgatcag cgtcgttttc aagccgtcca
    13741 gcaggtacag ccagcggtct ttctcaataa gagtggaatg gaatttattt ataaagttat
    13801 cgataaaatt cacggttacg atctcctgtt agtctttccg gacaatgata acctgtgtcg
    13861 tcgttgcata gccctgtgta aaggatacgt tttcctcacg gtccggtgta acagtcatgc
    13921 cggctacgcc gacatctgcc ttgccggact gtacagcagc aataatggaa tcgaattcca
    13981 tgttctccac tttcagttcc atgccgatct cgtcgcagac agcctgcatc atatccacat
    14041 cgacaccgac aatggtctct ccgtctacgc tctcataggg cggaaattct gcgttagtcg
    14101 ccataaccag tgtgccgtcc cgttctacgc tgtcgtccgg tgtgtaggac ttctggtctg
    14161 cgttatcgcc gatccagtgg ctgacgatct cgtccagtgt gccgtcctct ttcagcttgg
    14221 tcagagcatc gtccagcttc tgtcccagtt catcgttgcc cttcttgtag gcaatggcat
    14281 attcttcctc tgcaaaggga tcgtccagaa ttttcagggt atcgttcttt gccacaaatg
    14341 cctttgccgg ctcgctgtcg atgataacag catccagctt gccctgcttc agagcctgta
    14401 cggcatctgc acccttatta tacggttcta cggtggcatc ttctacatcg ccggcataga
    14461 catagccggt ggttcccagc tgtgcaccga tcttcttgcc tttcagatca tctgcactga
    14521 atacagtgtt ggcaggagtg ccgcatgccg tcagagatac tgccagcatc gtgcttgctg
    14581 ccagaagtgc gattgctttc gttgtttttt tcatatgtga acttcctttc tgtcagtcct
    14641 gtactgacat ggcttttatt gctgatacgc aattcttttc tattataatc gaaattcgca
    14701 aagaatgcaa gcggttttcc ggtattttcg gattttttgc gtttttgata tgatatgttg
    14761 ataaattctt tcttttcaca cgtgaaatcc ggttgacaga ccggaaaaac catgttataa
    14821 taaaaatgtt ttttgtgata atgtagtagt gctgtaaagg aaacactgca taaagcttgt
    14881 gcgtaagatg cgtagtcaga tttttcggca gattgcacag
//
LOCUS       DAWBGR010000046        14548 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1315_length_14548_cov_4.314842, whole genome shotgun sequence.
ACCESSION   DAWBGR010000046 DAWBGR010000000
VERSION     DAWBGR010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14548)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14548)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14548
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1315_length_14548_cov_4.314842"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3242 a         4159 c         3509 g         3638 t
ORIGIN      
        1 tcgccagacg ctctttgtgc ggtattgcct tacgcttcgt ccggtgtcag ggaaaccgat
       61 tccccgttca gaatcttatt ggaagtccgc attgcacaca tttttccgca catggagcag
      121 gtatgacgat cctgcggcgg acggctctcg aaataatccc gagccttttc aggatcgatt
      181 gcctgagcaa acatgccctc ccagtcgatc gcacagcgag ccttgctcat ggcgttatcc
      241 caatctcgtg cccccggaat gccccgtgcc agatcggcag catgtgcggc aatcttcgaa
      301 gccacgatgc cgtcccgcac gtcctgcaga tccggcaggc ggagatgctc tgccggtgtg
      361 acatagcaca ggaaatccgc accgctcatg gcagcaatgg caccgccgat cgcagctgtg
      421 atgtggtcat atcccggagc aacgtctgtt accagcggcc ccagcacata gaacggagca
      481 ccatagcaca gccgcttttg cagcttcatg ttggcagcga tctcgtccat ggtcatatgt
      541 cccggccctt ctaccattac ctgtacgtcg cgttcccacg cacgcttggt cagggcaccc
      601 agttccacca gttcactgat ctgtcccgca tcagtgctgt ccgcattgca gcccggacgc
      661 agagcatcac ccagactgat ggtcacgtca tatttccgca gaatgtccag cacgtcgtca
      721 tagtattcgt agaacgggtt ttcgttgccg gtcatctcca tccacgcgaa cagcagcgag
      781 ccgccgcggg aaacaatatt ggtggtacgc ttctgccgct tgagagcttc caccgcacgg
      841 cggttgatgc cggcgtgaat ggtctggaaa tccacaccct ctttggcatg tgcctccagc
      901 actttcagga agtccgttgc cttgatgtct gccaaatctt tttccagata gccaatggca
      961 tcatacatcg gcacggtgcc gatcattgcc ggcgacattt cgatcagctg tttccggaaa
     1021 gtatgggtct ttccgtagtt ggacaagtcc atgatcgctt cgcactgaaa ctgaattgcc
     1081 agcttgacct tttcgaactc tgccgtgtaa tcgtggcaat ctccggagat gcccagattc
     1141 acgttcattt tcgtccgcag tccctcgccg atgccatagg ggtgcaggct cttgtggtgg
     1201 atatttgccg gaatgcaaat ggtacccttt gccacccgct gccggatcag ttccggctcc
     1261 agatgttctt ccttggcaac agtttccatt tccggtgtca gaatgccctg ttttgcggca
     1321 tccatttgtg ttgcatatgc agccatgtat tctattccct tcttttatgt aatatgatct
     1381 ttctttaaaa ttgcatagtt atgtgcaatc ggtccgtgtc cgtgtcccag cgacaagcct
     1441 gccgcaatgg caccgctgat atacgccttt ccgcggcgga cactttcttc cagcgaagcc
     1501 ccctgtgcca gaaaagatgc aatcgccgag gacagggtgc agccggtacc gtgagtgttt
     1561 ggggcatccg tccgctgtcc ggaaaaccac gtggcagtgc ccgccgcata gagcaggtcg
     1621 ttgcagctgc ccatgctgtg tccgcctttc agcagcacgg cagtgtgata ggtttcggac
     1681 agctgtcggg ctgcctgctc catttgcggc ggcgaagtga tgcttgttcc ggtcagctgt
     1741 tctgcttccg gcagattggg agtcagcagt tccgccagcg gaaacaactg ttccgtcaac
     1801 gcctgctgtg cctgctgttt cagcagcggg tcaccgctgg tggcaaccat gaccggatcc
     1861 accaccacat ggggcagctg atactgtgcc actgcttctg caatggcgag gatctgcggc
     1921 gtatcgtaca ccatgcccac tttgacggca gccggcggaa tgtcctcaca gaccgctgcg
     1981 atctgctgct ttaaaaattc tgccggaaca ggacaaatgc cctgcacccc cagcgtgttc
     2041 tgtgcagtca gtgctgtaat ggcactcatg ccgaaacagc ccagcatggt catggttttc
     2101 aggtctgcct gaacgccggc accgccgctg ctgtcactgc cggcaatggt caaaaccgga
     2161 gcaatcgtca tcttccctgc tccttctgct gaaacggtgc agtcagttcc cgcattgcct
     2221 gtgcggcagc ttccacgttc ggctgtccga acaccgcgga aactactgcc acgccgtcaa
     2281 tgccggtatc gtacagcttc gggatattct gggcattgat gccgccgatc gccaccaccg
     2341 gaatggacac tgcctgtcga atatcgcaga gtacatcatg gggcagaaaa gtggcatcgt
     2401 gcttggtctg ggtgctgaac acagcaccac accccagata agttgctccg tcccgttctg
     2461 cctgtaacgc ttctgccaca ttgtgggcac tggttccgat gatcgcctga tctcccagaa
     2521 tctcccgtgc ctgctggact ttcatgtccc gctgtcccac gtgcacgccg tctgcaccga
     2581 ggatttttgc cgcttctgcg tcatcgtcga taatgcaggg aattcccgca tcacggcaca
     2641 gctgcaaaat gggatatgcc agctcaattc tccgtttggt ggtacagttt ttttcccgaa
     2701 tctgaagcat ggtaatgccg ccggcaatgg cacgctttac ctgttccgcc agatctgccg
     2761 ccggtgcagt caccgcgtac agctgataga tcattggatt ttgttgcatt gaaattctcc
     2821 tttctgtcag gcagcaccgc acaagttttt ttgcagtgct gccataattt ccttttgccc
     2881 taggcaatct gccgaaaaat ctgactacgc accttacgca caagctttgt gcggtgttgt
     2941 cctaaagaaa aagcgtaccg caaaggcacg cttttccaca aaatatcgtg tgtcgttccc
     3001 ttcgttggca ttatccaaat caggtgcggt cgagatgtct gcatctcctc tcagcccacg
     3061 cacagcaagc tcccgtgtat catgttgttc cggtttcaga ccgtgttcgc cgaaggcttc
     3121 cggcgaaacc gtgccagcat gtgccgggca cccagcccga aaaactccag cattgccgca
     3181 tacttatcca caaaagtaat ggcatagctt tccttatagt ggggcatgcg gtgagtcagc
     3241 ggccacatgt gcttttcgat catgtcccgt tcccgcgggt tcagggcaaa ccggactgcc
     3301 gcattctgca tggcaacttc cggatgatgt gcactgtggg acttctggtc gggagaatcg
     3361 gaacggtcat agtccgctgt ttcataaaag tacagatcat gaagcagccc tgctcttgct
     3421 gccgaaaccg cgtcccagtg gaaaaagcgg cagagcaggt aattgtaata ggaaacattc
     3481 aggctgtgct gaaaccgtgt ggtataatga tgatgacgaa actgtttcaa cgcctgtacg
     3541 tctgcatgtt ccaagatgtc tgcgaccagc atgtaatatg cctgccgcgt cagttctttc
     3601 ttcgagcctg ttgcatgaag caatacacgg caccgtcctt tctgcaattc cgcaaagaac
     3661 cataggtgca cccgtttgtg cagcctacct ctattttagt gtaacagatt tcatatgatt
     3721 tgtcaaggga atttttatgg cagcacacaa atcaaagcat ctccattcga aaaaatccga
     3781 caaaaacccc atttgtatct tgcacaacct gttctgtcat gctataatag agaaagcaat
     3841 attgttttat tttgtcttta tcgaaaggaa cgtactatgt cagttcgaaa atcgctacgc
     3901 tgtttcagca ttcttctcgt ggcagtctgc accctgccgc tttgcaccgg ctgttccata
     3961 ccatttctgg aacaggaaga aagcgacggc tccggctatc tgtttaccgc cagccttgct
     4021 gccaatccca aaagtctgga cccccagtct gccaccgatg ccgcttcgaa aaccattatc
     4081 gaaaacctct acgaggggtt ggtggagctg gacgaaaacg gctccccgca gctggcggca
     4141 gcggaaaact acaccgtttc tccggacgga ctgacgtaca ctttcacgct gaaaagcgac
     4201 cgcttctggt tctatgatgc caatgcggac gatgtcgtgg acgacgacga aaagtggcag
     4261 gtcactgctg acgactacgt ctatgcgttc cagagaatct ttgatccgca gacacaatcc
     4321 ccctatacca gcatgttctc ctgtctgcaa aatgccgacg cggtgcagag cggacagctg
     4381 gcaccggagg agatcggtgt ccgtgctgtc agcagtaccg tgctgcaatt ttccctgtcc
     4441 cgtccggacg cggaattttt cagcaatctc gcctccactg ccgccatgcc gtgcaacgaa
     4501 acctttttcc agcagaccaa gggcagatac ggactggaca aggattctgt tgcctcctgc
     4561 ggggcattct atctccgcct gtggttctac gacccctacg gcaaccagaa ccagatcttc
     4621 atgcgtcgca attctgtcaa cgccgaagca cggcgggtgt accccaccaa tctgacattc
     4681 cagattcgga aaagcagcga tgaaagtgcc gcagattttt ccaacggcac agcagacctg
     4741 tttatcagcc ctgtttatca ggcacaatac atggagagca gcaactatgc cgtcaccgcc
     4801 agccgtgcca ctacgctggg actgatcttt aacccaaacg atgttgcttt ttccaaccac
     4861 aagattcgcc aagccctttc catgggcatt gaccgtgcca acattggcaa gaactccaac
     4921 ggtgatctgc tgccggcata cggactgatc ccgccagcag tccactggaa cggcactgca
     4981 taccgcgaca gctatccgga gacacagact gcctatgatg ccgacgctgc ccttgccctg
     5041 tttcaggagg gcatgcagga gatcggcaag gaatcgctgg attccaccaa gatcctggtc
     5101 tgctcggctc tgatggactg cgaaaatctc cacgacatca ttcagacatg gcaggaggtt
     5161 ttcggttttt acatcggcat tgaagaagtt tccgagtcgg actactggaa acggctcgcc
     5221 gaccagacct ataccgttgc agtctacggc atcaccgccg gttatgacag ccccgccggt
     5281 gtactggatc agttttactc ccgcaccaac cagttctatt atgccaacgg ctctgtcgat
     5341 gcggcgatca acgccctgca ccagtgcagt tctgcggaag cactgcagca gcagtgtgca
     5401 cagctggagc agaccattct gaacgaattc tgtttcctgc cgatcttcta caagaaccag
     5461 tactgcatca ccaagagcag caacaaggac attggctacg atccgttttc cggtgcactg
     5521 aatttccgga acgccaagca ttttgaatag ttccagaaat ttccttgttc ccttttggac
     5581 tctgctgcat acaataagaa taccagcagg gcctgctttg aaatagatgc tgcttatcag
     5641 caaagggctg ctgccggcaa tgtgttttcc gcacatgtcg gcagcagccc ttttttatgt
     5701 tttaaggcaa tactgaataa agcttgtgcg taagatgcgt cgtcagattt ttcggcagat
     5761 tacgtgcaaa gccgtcaggc aggctttatg cagtgtttcc ttaagttaat cggccccgcg
     5821 gtctttttcc cgcagacgtt tttcaatgcg ttcccgatct ctctgggaaa tgcccttggt
     5881 ctgtttcagc ttccgcagtt ccgcttcggt ttttccatag aagtccttgg gattccggtc
     5941 gttgtcattt cgcatatctg ctgcctccta tgcctttttg gtcttccagt tctgcaaatc
     6001 attttcgaac cggttcacct gatcgtgtct gcccaccact gccagaatat ccggtgcccg
     6061 cagaacagtc tgcggcgtga tgtttacagt cacttcttcg ccacgcttca atgcgatgat
     6121 gttcagcccg taccgctgcc gcacattcat ttcctgtaca gtcatctcct gtgcagattc
     6181 gggaatggaa atttcggtaa tgctgatgtc gtcgttcaag gcaatgtact ccataacgcg
     6241 gggagccagc agccggtttg ccagacgaat ccccatttcc cgttccggat atatcacttc
     6301 agcaccgatc ttttccagaa cgctgccctg ttcctcggtc aacgccttgg caatgacatg
     6361 gggcacaccc agctgcaaga cgatcatcga ggtcagaatg ctcactgcca gattgccgcc
     6421 gataccgata atgactgtat cgcagttctg aatgcccaca ttccgcaggt tttccttgga
     6481 cagatctgcc atcagatagg cgttgtccgt aaacgccacc gcatcattga tcaccgtttc
     6541 gctcttgtca atgacgatca ggtcttttcc ggattccgcc agacgtttcg ccagtgcaaa
     6601 gccaaaccgt cccagtccga tcacagcata taatttttct tttttcgaag aatttctcat
     6661 attattcacg ctcctgttat agtaaggtca gcagtggagc aatttcagac aacgacttgc
     6721 cattgtaaat tttgtcggtc atcaatgccc caatgctgtc gtacactttt tcaatatctt
     6781 cgtagctaag caaaaatttg tgtatatgaa acgggttaat aaaaattgta tcatgttccg
     6841 gataatccaa caagacatca ttggtaaaag acaatatcac gtcctgcaat tcctcttttg
     6901 tcataacacc tacctccata tttggaatta tcccacagaa atagtttctt ccggtcttaa
     6961 aatctcgctg tttcttctgc cctgcacaat ccacagcgtt gccaccgtca gcggtcccag
     7021 ccgcccgatg tacatcgtca aaatcagaac cagcttgctc agaccggaca gtccaccggt
     7081 cagcccgcag gaaagcccca cggtcgcaaa ggcagaggtg gcttccatca gcacctgaat
     7141 gaacgaagca tctggttcca gcatacacag cagaaatgtt gaaaccagca ccacagacaa
     7201 ccccatcaga aaaatcatca gagccttatc ccgcacttcc tgcgaaaacc gccgccggaa
     7261 cacagtgggg tgctgatttc tggtataggc gatcgccgac cagatgcaga cgaacagcgt
     7321 ggtcaccttg atgccgccgc cggtagagcc gggagctgct cctacaaaca tcagcagaac
     7381 catgcagagc agcgaggcgt tggagaagtc ccccagatca taaatggaga accccgaagt
     7441 ccgcgtcacg gcactataga aaaaggactg cacccatgtc aatccatgct gtgtggcgtg
     7501 gagcagcaaa gtgcctactg tcagcagcag cacagtcatg gtcagcacca ccttggtgtg
     7561 cagcgaaaac cgccggaacc gccgcttctg tgcgatgttc agcatcacca caaatcccag
     7621 tccgccacag atcgtcagtg ccgtggtcag cagcagcatg ggcacgtggt tccggtacac
     7681 cgcaaggctg ctgccgccga tcaggtcaaa gccggcattg ttgaatgcgg aaacagaatg
     7741 gaacacactg agccacactg cctttcccac cggataatcc tgcacaaaaa tcggaaagga
     7801 cagcacggct cccgcaccct caatgccaaa tgtcaccatc agcattactt tcagcagccg
     7861 cagaatgccc tgaaagctgg gctggttcaa agattccttg atcagcgttc gttcccgaat
     7921 gccgatcttc ttgccggtca gcagaatgat gcctacaccc agcgtggtga tccccatgcc
     7981 accgatctgg atcagcagca agaggactgc ctgtccgaat ccggaaagca ctgcattaca
     8041 ctgaatcgag gaaagaccag tgatgcacac gccgcttgtg gcagtgaaca gggcatcgat
     8101 aaaagacagc gactgccccg actggtgcga gatcggcagc atcagcaagc ccgttcccaa
     8161 taaaatcaaa aaggcgaatc cggcggcgat gacacgcccc ggcgtaaaaa aacgattcca
     8221 tctcatatgc tgttctcctt aaaagaaaaa gccggcacac actgtccgtg tacaccggct
     8281 tttttcgaaa tgcattcagt ccaaatccat cgtcatgctt caaacgtcaa agccattttt
     8341 ccgaatgcaa aatcacaggt ttactgtgca gtatcttctg tttctgcact ttccgcagcg
     8401 gcatcggttt cctccgaaga aaccggttcg gcagtggttt catacacccc tgccggtgca
     8461 gcctgagccg acttgcgagc ctcacgctct gcctgttccg cttctcttgc tgcacggcgt
     8521 gcagcctctg ctgcttctgc ctctgctttc catttgccat aggactcgtt cttatggccg
     8581 cactccggac agaacttctg cccgtcgttg accttagaac cgcattccgg acaaatgctt
     8641 gcgttgtcca gccctgcaag ctccgacttc agcacatcga tctgtgcgat caggtcagaa
     8701 gcctgctgga acagaccttc gtactgctcc tccggctggc actggttctc cacataatag
     8761 cgggaaccga tttcctgata aatggtctgg agcgactcat tacagctgtt gatcatgcgg
     8821 cgtacccgtg cacgggcagt cgccttcttg gaagtatctg caacttccct cgccattttt
     8881 cccacgtcgt cgctcatttt ccgaaactga tcacgaaaat ccattttatt ctcctctaca
     8941 aaagcgttct gctgattttg atccgatcat tgttattgta tgacagtccc ctgccatttg
     9001 attcccgaaa cacaacttca aagcaacgtt tcaaaacaag cagtctgccc tatggtatta
     9061 ttcgattttt taaggaaaca ctgaataaag cttgtatgtg caaagccgtc aggcgggctt
     9121 tatacagcgg ttccttatcc gcaaacatat gcgaatctgg cactgcgaag tcccagagtg
     9181 cttctgaatg cgtcgccgtt tactgttttc tgaccgtcga tgtttaccga acggacatag
     9241 ccgctgtcgc cgacttccag ccggatccag ttggacggat ttgctgaaag agaaacgcca
     9301 taggcattct gaatacggga gcgtacttcg tccacagaca tataggtgac cacaccataa
     9361 taaggatcgt attccgcatc gtattcactg gggacactga ccagataatc atactgtctg
     9421 ccccagatgt ctgccgcact ggcagtcgca ccgccgctgg atgcaccata aactgtcaac
     9481 gcatagcttc cgtcataata cagtgcctgt ccggcgactg cggaaactgc atcgatcaca
     9541 ttctgcggcg gattcggttt caggatcaca ttgttgacac tgccgccgtt aaacatgatg
     9601 tatgtataag cggcgattgc ctgtgccttc atggcttccg gatgcatggt agagctcatc
     9661 tcgttgtata cgatctgaga aataatatcc acatagctgc cggtaacacc gttggcagtc
     9721 acggtgctgc ctgcaccttc ctgcaccagt gtggttccgg ggaagtagtg gaacaaaatc
     9781 gactgatagt cataaccgcc gtaagtggcg taattattcg caccgttctg gctcatgcca
     9841 acacaatgtc catatccata agtggtaaat gcaaactgac cggcactgac agatgctgcc
     9901 atagaggcgg ctgcaccgat gtttgcactg ccctctgtgc cggaagaacc gatcggcacg
     9961 gtgacttctt tcagcgttgt cgcattctga attgccgcag cctgttcttc cgacggtgta
    10021 acttcttcat actgaaccaa agataagctg gaatcgtaat ctgaaatttc agctttccac
    10081 aaagtatcgt cgctttcgtt tgccgcagtt gtcgtgtcag tgacagcagt actagtacct
    10141 gctgtttctg tttccggcac tgcataggca ctgacggcac aggttcctgc cagcagtgct
    10201 gcaactgcaa gtgcaatacc catttgtggg taacgtattt tcatatcaaa actccatttt
    10261 taccgacaaa atgccgggta gtaaaaggcg attctcatcg cccgaaagcc gcagacattg
    10321 ctgccgcggc tttctgctgc acagcagtac cgtctgggtc aagccctccg gtcgtgtcac
    10381 tcctgtgcag cggcggtttc actgctgccg ccctgaatgg ttttcagcat ggacgcagta
    10441 tcttcgccgt cgcgaagccg gcgtgccacg atgacaaacc gtgagttatc ctcatcggaa
    10501 tagcaggtgg agagcgtcag caggctgtcc ccatactgca cgtcaatggg attgtcgatc
    10561 atattcttcg cgttgacaga acccatgaag ctgtcaaatg tggtcttgtc gtccagttcc
    10621 tccatttgcc agtacttgaa atctgaatcc gcattgccgc tggtaataat cagaccaacc
    10681 atgacatacg tatagtccgc atagttggag ctgaatgtga taaacggtgc ttccttgtaa
    10741 taggacgggt tctgacggta ctgccgcaga gagccgaaca tggtgttgtc tgccatgttg
    10801 tgaccgtaca ggacaatgtt ttccgaccac tgatccggat aggcaccgaa cgaatcagtg
    10861 cagtccatga atacagtgcc ggcacggaat tcctgtccgt caaagccgtg atccagataa
    10921 tattcattat ccgcagtctg taccacaggg ttgtccacct gtgtaccgtc aatggtgata
    10981 taaccgaccg tatccggatt ctgagccagc agctttttgg cacgctctgt cagttccggt
    11041 gccgcagttg cggcctccgt cggtgcctct gtggcaggtg cagaagttgc aatgcccgca
    11101 ttctgatcga tctgttctcc gcagcctgtg gcaaagagag aaaagaccaa tgccccggcg
    11161 actgcaccga tttgccattt cttcatattc gaattcctca tttccattat cgcccttctg
    11221 catcaaccgt aaggagcaaa aggggcatca gggttgtatg tcttttcatt ccacaaataa
    11281 tacacattgg gccatttgat gttgggattg agtatgctgc cggcgattcg ctcgttgggg
    11341 tcctcgccgt cccgcacacg ccgtgccacg atcaccagac gtgccgtatc aaaggcatta
    11401 ttgcaggtgg aaagtgtcag cagctggtcg ccgtatttca catctacatt cgtgatgcgg
    11461 agcgtccgcc gcttgacttc gttgacatag ttataaaatt ccgtttcatc tgcaaaatca
    11521 attgcgttcc agtagtcaaa acgagtgtcc gtcgtatcct cggcatctgc aataaagtag
    11581 ccgaagatct tatactggta ggtttcgtaa ttggaattca gcgagatcag cggatgttcg
    11641 tcataatagt tttcatcggt acggtaatat ttcagcgtac caaacatgga aaggtcacgc
    11701 atgttgtgcc cgtagatgat caggttgtcc gaattcggcg tttgcagagt gccgtcctca
    11761 cccacgtcat cgaacgaaca gcggtagtcc agaaagatcg agcccggctt ggaatcctct
    11821 ttctgcacat tccggtagag atagtactca tttcccggct catcgtccag atggtgcagc
    11881 accggataat ccactttggt acccggaatc gagatccagc cgttgatctc tgaattcatc
    11941 gccagcaggt ctgccgcacc cggcagcagc gtgtagatct tttccgtagg ttcttctgat
    12001 tccggctgcg gctgagtgag ttccgtggtc tgggtggcaa agttggcatg atacgccgaa
    12061 ccaatgtcgt catacatctt gtctgcacgg aaaatgtcca caaagtacag cacgatcagg
    12121 acaacgcaca ccagaaacac tgccgcggaa aacaggaaaa tactctttcg caccacttcg
    12181 cctcccgaat ctccccgctg cggaaacaga tccagcatct tctgcccgaa cgttttcttc
    12241 ttcggtttcg gagacacgcg gggcggtggc ggcgggtatt tttccggatg ttctttctgg
    12301 tctttgacct gtcggatctg atatgccacc cgcagggaaa ccgcttcttc ttcctcttcc
    12361 tgtgcaatgt ggaacagcgt tgtccgaatc gccttgatct tgtctttcgg ttcgctgggc
    12421 gttttgctct tggcttcgcc tttttctgaa gaatcggttt ccggttcttc cggcagcaca
    12481 gtccgggaac gctctgctcc cccggcttcc ggcggtgccg gtgtttccga agattccgca
    12541 gatgttacag cggttttttc ttcttcctgc attctgtttc ctccatcagg atccgttctg
    12601 cgacaccaaa tatgcacaca gcggctccgt gacggatctc ctcccgcgtg tgcagaccgt
    12661 atcgccaaag ctgcaggtgc atgacatatt cctgtttgcc atacaggatc cctgcataga
    12721 ctgttcccac tggctgttcc ggcgttccgc cgccgggacc ggcaagtccc gtcactgaca
    12781 gacacaaatc cgcctccgcc tgtgttcgca gtccctgcac catggcagaa gccacttctg
    12841 cactgaccac gcctttttcg gcgatcagtt ctgccggaac gcccagcagc ttggttttca
    12901 tgcgttccgc ataggtacaa agtcccagtt caaacacatg ggacgctccc gcaacagaag
    12961 tgatcttctg ggacaacaga ccgccggtac agctttccgc cgtagcgact gtctgatgtt
    13021 ctccatctag taattgtaca atatgtgcaa gggttttgtc aagtactttt tctgtatcct
    13081 gatagatacg gcacaatttt tcttcatcta acaacgtata tgccatattt cacagcacct
    13141 ttccattccg ccggttcggc aatcacactt tgtgcggcgt ttttctaaga aaagaaaagt
    13201 ttcagagccc gtccctacaa aaattttctg tggtttttaa gacagctcga acactttcat
    13261 cgttgtgcct tccacagcct tgttgaccat ttctgtaaaa tcgaacttgc cctgtgcctt
    13321 ttccacttcc tccagggacg gtctgacctt ggggtgcttc ggagtaaaca cggtgcagca
    13381 gtcctcatag ggcagcgtgg aggtttcgaa tgtgccgatc atgcgggcag tttctacgat
    13441 ctcggtcttg tccgaaccga tcagcgggcg gaataccgga atcctgctga ctgcatcggt
    13501 gcaggcaatt gcctgcatgg tctggcttgc cacctgtccc acgctctcgc cggtaatcaa
    13561 ggcaaggcaa ttgtcctttg ccgcgatttt ctgggcgatt tccatcatca gccgccgcat
    13621 gatgacagtg aaatattcct ccggacaata cttcttgatc gcctcctgaa tctccgtaaa
    13681 cggtacgcag aaaaaggcga tgtttccgca gtacggagtc agttcctgac agagctgctc
    13741 caccttttcc tgtgcacggt cggaggtata cggcgggctg acatagtgaa tggcagaaat
    13801 gtgtacgccg cggcgtgcca tacgatagcc ggctacaggg ctgtcgatac cgccggacag
    13861 caggagcagt gccttgccgc tgctgcccac cggcagaccg ccggcacctt tgagatttgc
    13921 accgtgtacg aatgcgtgcg tgtcgcggat ctctaccata acagtcacgt ccggatgacg
    13981 tacatcaaca gacaggtgcg gatacgcatc cagcagaatg ccgcccagtt ccgcacagat
    14041 ctccggcgat ttcagcggga acgccttatc tgcccgcttt gcctctactt taaatgtctt
    14101 tgctcccagc agaacttctt ccagataagg cactgccttg gcggaaatgt cctccatggt
    14161 cttttcgcag acacaggcac ggcacagagc cgcgatgccg aacacacact gcaaatgctc
    14221 cagcacctcg tccagatcga tgtcctccga cagcggttcc atataaatgg tggactgggc
    14281 acggttcagc ttgaatgcac caaacttttt cagacggcga cgtaggttct tcatcagaat
    14341 gtcctcgaaa gtaccgcggt tcagcccttt cagtgccatt tctccgtatt ttgccaaaat
    14401 caattctttt gccattggtt ttctccttct atggaaacac tgcatcaagc cgcctggcgg
    14461 ggcttatccg tgttttccgg tatttcaggc aataccgcac aaaaagcgtc tggcgacttt
    14521 gcacgccatc tgcttgcaaa tttccctt
//
LOCUS       DAWBGR010000047        14423 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1326_length_14423_cov_4.965479, whole genome shotgun sequence.
ACCESSION   DAWBGR010000047 DAWBGR010000000
VERSION     DAWBGR010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14423)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14423)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14423
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1326_length_14423_cov_4.965479"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3492 a         3968 c         3759 g         3204 t
ORIGIN      
        1 gtcgcacaat ctttgtgcgg tattgacttg aatcaggctt aaagcctgcc ggaaacaaaa
       61 aagtctgtat gcctcacggc acacagactt ttttcatttc aaaacacagt ttcccgaaag
      121 ttccaatcag ttgataatgg tttcttcggt ttccttgtcg aacaggtgaa tcttgttcgg
      181 atccagagca atcttgatct catcctgcgg acgagcagta gaacgcggat caacgcgggc
      241 agtcagagaa acgccggcac aaaccagata cagataagtt tctgcaccca tcatttcggt
      301 gatttctaca gttgcatcta caacaccagt ggaaacgttg gacaggaaca tttcgtcatc
      361 gtgcagagcc tccggacgga tacccagagt cacttcctta tccacatact ttgccagttc
      421 cggtgttacc tttgcagccg gtacttcgac ctgatacttg acagccttgc tggtcttggt
      481 agcttcagca ccgaattcta catagtagtt gtctgccttc ttcagaacag catcgatgaa
      541 gttcatctgc ggagagccca ggaatcctgc aacgaacttg ttgcccggac gctcatacag
      601 agcctgcggt gtatcgatct gctgaatgat accgtccctc ataactacga tacggtcacc
      661 catggtcata gcttcggtct ggtcatgtgt tacatagata aatgtagtac ccagcttctt
      721 gtgcagcttg gagatctcag ttctcatctg tgcacgcagc ttagcgtcca agttggacag
      781 tggctcgtca agcaggaata cctgcggatt acgaacgatc gcacgaccca gtgcaacacg
      841 ctgtctctga ccaccggaca ttgccttcgg cttacgatcc agcaggtggc tcagatccag
      901 aatctttgca gcttcttcta ccttgcgagc gatctcgtcc ttcggaacct tacgcagctt
      961 cagaccgaat gccatgttgt cgaatacggt catgtgcgga tacagagcgt agttctggaa
     1021 taccattgca atatctctgt ccttcggagc gatgtcgttt accagacggt cgccgatgta
     1081 cagttcgccc tcagaaattt cttccagacc agcgatcata cgcagggtgg tggacttacc
     1141 acaaccggaa ggacctacca gaacgataaa ttccttgtcc ttgatctcca gatttacgtc
     1201 ctttacagca actacattgc ccggatactt tttataaata tgtcgaagtg ataaacttgc
     1261 catttgcaca gttcctccta aataagattt tccagaacgt ccccgcagct gagccgcttg
     1321 ccgttcccgg tgacatatat atcataccaa aaataaaaac atttttcaag tcgtttattg
     1381 cccaaaattc tgcttgtaca tttatccatt atgacgaatg actttggagc gtttgtagca
     1441 aacttgcaaa atcggtttca catttcaaca atttttggac atctttttca gacagcacac
     1501 aacacgctcc cagcggcaat tccggcggca gcagcagtcc gcccattgca acacgtgcca
     1561 gttcactgac atgatttccc agtgcggcaa acatgcggcg tacctgatga tacttcccct
     1621 catgcagaca gatgagggct tcccgatcgg tgtcaggaac cggcgctgcc tttgccggca
     1681 ggcactgtgc cccgtctgcc agcgtgatgc cctgttccag cggctggaga tagccgtcct
     1741 cccacggacg tgccagcacg atgtgataat acttcgccac atgcccccgt gccgcgatca
     1801 cctgatgggc aagctgtccg tcatcggtca gcagcagcat gccggtggag tccttgtcca
     1861 gccgtcccac tggtcgcaga tctttccggc acagttccgg cggcaccagt tccagcacgc
     1921 ttttctgtcc tgcctcatcg gcggagctga catagccctt gggcttgtgc agcagcacat
     1981 agagatactg gtcgccgcag atctcccgtc cgttcaggca gaccgtgtgg gcttccggct
     2041 gtactgcctg atcggctttc cggacaaccg caccgtctac ggtgactttg cctttttgta
     2101 tcatctgccg cagttcccga cgggaataca tggtacgggc agagagaaac tgatccagtc
     2161 gcatagaggg acctccgttc tatttttcat ttggttacat aagctgtaca agcaacattt
     2221 tctgaaaggt ggtttttaca tatgggaaaa cgcagtgtac tcgctctggg aatcgcaggg
     2281 gtattgttcg ggtgcgggtt tttgtggcag tggctgcacc agccggaagc agaaccggca
     2341 ccgcaggaga tcgtgctctc cgacggagca cagcggctcg cctttctgga ggcgaacggt
     2401 cagccggaac gctgctgtgt tgccacagaa acggtgcagc tgcctgccgc tgccgatgat
     2461 acctaccgcc gctatctggc tctccagcag gcacaggggc tgcctctggc ggcacatctg
     2521 ggagaagccg ccgcccgata cacttacaca cagaacagct ccggacaggg ttccctgtgc
     2581 acagagctgc ttcttagtga agatcaggtt ttgctgggag cgatccaata cgacagcacc
     2641 gcaccggaac agttctcccc cgtcctttcc acagaataat tacttgtcaa atacgtgcag
     2701 cagattgccg atcaggggca gctgctttgc cttgccattg gcagcgttga ggattccaat
     2761 aattatcaga gccagaatcg ccagagaaag cagggcaggg agaattctgc ccagcaacgg
     2821 aatcttcccc agaatcgcat tggcaatgac aaccacagct tccgcaagga acaggataaa
     2881 gccctgattg gcgtggtact tggcatagcg ggactgcccg ccgtttacca gcaacggcag
     2941 aatgaacagg attcccacgt aagacagagc cgcccagatt ttgttattct gcacgtcctg
     3001 tgtgtcatac agcatggaat cgtcctgcgt gttcatgaag tcgttaaaat ctttactcat
     3061 cgtttttcct ttccagtcaa gatgcagcat tctctgatgc atcacttttc atctgtttta
     3121 tccagtgcac cgatcaccgc cagaaagccg gcaacgtcgg tgaagtcctg atacaccgac
     3181 gcaaaccgca cataggcgac tgcatcgata tatttcaggc gttccattac cgcattgccg
     3241 atctccgaag aagtcacagt actccgcatc tgccggctgt attcgtcctc cagttcttct
     3301 accatggcaa cgatcagatt agagttgacc ggtcgtttct tgatggcgtt gaaaatgcct
     3361 gtcaccagtt tatgccggtc aaagggctca taagaaccgt cttttttctg taccatcagc
     3421 atgggacggt ctacggcttc ataggtcgta aagcgtttgc cgcagcccag acactcccgc
     3481 cgccggcgaa tccgttcatc gtgaggacga gaatccagca cacgggtttc cgtgtgcccg
     3541 caaaatggac aattcatgac accacagttc ctttctttcg ggcagcaccg cacaagcttt
     3601 gtgcggtatt gccttaacgt tttccctcaa tgcggtttcg cagcagcgtg taattggcgg
     3661 caagttctgc cacatcagaa aagccgctgc ggtatagttc caggatcaca gagcaatccg
     3721 gactgactgc cgccagttcc tttagaaatg catcgaaccg aaaccgccct tttcccagcg
     3781 gcagacagtc cccgtattcc ccgtggtcgc ttatgtgcac atgcacgata tggctgttca
     3841 gcccgcggag catggcgaaa ggatcttctc cggaacgcac tgcctgcttg atgtccagca
     3901 caaagcgtgc ctgtttgccc agtgcgtccc gcatctccag caaaaacggc agcgaggcac
     3961 tggtacagcg tgccacattt tcctgtgcca cctgtacccc gtaggattcg cccagttctg
     4021 ccagccggcg gaaccgtgta aagtacatgt ccttgttcac cgaagccgca ggcaccttgt
     4081 tgccgtgcag aacaaagacc tttgccccca gctgctgcat ggcggtgaaa taataccggc
     4141 agtattccag ataatcgtcc acacgccttg cgtagttgga gaacagcatg ttcggctcaa
     4201 tctcacaggt gtacgggtgc agcgaagcac aggtcatgtc aaaccgctgt aacagccgtg
     4261 ccagcgtatc cacaaagctg cggcgtaatt cggagtgact gttgatgaag atttccacgt
     4321 gggaaacgcc ggaaagtgcc agatcataca gggcttcttc caccacgcgg ggataaagac
     4381 acgccgtaga aacaccggct cgcagcataa gatcactcct tccagtttgt tttcctatta
     4441 tagcacatca ccgcggaaat ttcaatagaa agacgcagat tttcaccaat ttttcagtca
     4501 gggcagcact gcgtaaaaaa caaaaaccgg tacagacaca acgcctatac cggtttttta
     4561 caattttgga atcacgaaca agttatacgc ttgtttttgc ctttttcttt gcaggcttca
     4621 gcacggtgat gccacgcttg gtctgccacc atacccacag gctgggaaca aagcaaatgg
     4681 agttaaaggt acccattgca atacctgcca tcatcggtat ggagaagctc aggatcgaat
     4741 ccagtcccat taccagtgcc acaatggtaa ccaccagcat ggaactgaat gtagtaacgg
     4801 aagtccgaat gcttcgacgc agggactggg agtcactgat gttgaccaga tcacggatct
     4861 ggattcccgg cagcaatgtc tggttttcac ggatacggtc gtagataacg atcgtatcgt
     4921 tgacagcata tcccaacagg gtcagaataa ctgccatgaa gttggagtcg atctcaaacc
     4981 ggcacagtac cacgcagccg taagtaacga ccagagtgga aagcagtccc agcacggcac
     5041 aaacgccggc ggaccagccg ctgatctgct tgaatcggaa tgcaatgtaa atgatcagga
     5101 tcagtgcggc aaaaacagct gccacaatgc actttgcgaa gaattcgccg ccggaacgtg
     5161 cattgacatc gtttgcatcc agcagtgtca cttcattgtc cggacagagt tccttgacct
     5221 tgttggtcac gtcgttctgg cggtcaatcg tcagaccatc gttggaagtg aaggacaggg
     5281 acagggtctt ggtgtcgctg tccaggctgt caccctgctg taccttgacg gtagagccta
     5341 ccagactggt cagttccttg gaaatgccgt tctcgtccac atcgcctaca tagctgtaag
     5401 tgataatggt accgcccttg aattccagtg ccacattgac accagtaaag gtggaaagca
     5461 atgagatcag aatcaatgca aggccgataa tgaagaatac tttcgacttg ctgcaaaagt
     5521 cacgcacttt tacgtcaaca tcaaaatgct ttgcctcttt tgcttcttta gtctgctttg
     5581 acatcgatac cacctccata aagtttcttg ttctggaagc acttgaagcg agagagcgac
     5641 atcgtcatca gtcttgcagc aaatacgccc atgatgaagt tcatgatcag accggtcatc
     5701 agtgtgaagc cgaaggagta gatcacgccc tctgtggtcg caccgaagaa ccagaagatc
     5761 gtatggttga agatctttgc ccagatgctg ctggggacac cgaatgcacc catcagtacg
     5821 atggaaacca gaatacctgt gaggtttccg tcgaagatcg cactgaatgc acggtgataa
     5881 ccggagtaca gtgctgtatc cagcgtcttg cccttccgca gttcttcgcg gatacgctcg
     5941 ccggtgatga tgttcgcgtc aacgcccatg cccacagaca ggataatacc ggcaataccc
     6001 ggaatggtca gtgtggtgct ggacataaag ccgaaccagc cggaaactgc tgccagcatg
     6061 ccggcaacct gtccgaccag ggtgagcgaa gccacaacgc ccagcaggcg gtacagcacg
     6121 atcatgtata ccacgatcag aagcagtgtg atcagaccgg cgatcaccat agcgttcagt
     6181 gcaccctcac ccatagtcgg ggagatggtc ttgaagctgg tggtttccag agagaacggc
     6241 agtgcaccgg aattgatctt atctgccagt tccttggcac tctcataagt aaagttaccc
     6301 gaaatagaag ccttaccgtc cgagataact gcactgacag acggtgcaga gatcatggta
     6361 ttgtccatcc agatcgagat ctgacccttg gactgataca gttcagaagt tgcctcatag
     6421 aacttttcct taccgctgtc gttcaggtcc agtgttacca cccatgtgat ctcaccggtg
     6481 gcatcgtcct tggaaccgcc ggtagatgcc tgtgccacat cggcaccggt caggacgatg
     6541 tcgcctgccg gaatggtatc tccgttttca tcggtagaag tatcggtacc cttgcggaaa
     6601 gtcagttctg cggtttcgcc cagttccttg acagcctgct ccggattgaa atccgtttcg
     6661 cccgcctgcc acggataccg cacgataata cggttgctct tgtaatccag atacgtttcg
     6721 ctgtcgctga tgttctggtt tgccagacgc agcttgatgg tttccagtgc tgcgtccatt
     6781 tgttcctccg ttgcatccac atcgcccgcc ggttcaaagg tcacgtctac gccgccttga
     6841 atatcgattc caagacgaat gtcgccaaca ccgtgtacat aggtcttgac aatatcacca
     6901 tactgcgaat gtacgccgaa tatggtggaa agcgtaaaca gagcgatcac ggcaaataca
     6961 atgaagaaaa caggtttttt cacttttctc acgatttagc ctcctgtgct gcggtcctgt
     7021 ctgtcccagt accgcatatt aaactgtgcc gtcggcagaa tgcacgatcg acaattacct
     7081 attattatat tccaattttc gcaattagtc aataggtgcg gcaaaacttg tacaacattg
     7141 ccgatttgaa agggcttttt tcggcgactt ctccaaaaca ccggacaaaa cttttcgaat
     7201 tgccgttgca attcagggat acttatgata taatcaaaag gaaacaaaag gaaagacaac
     7261 accgcacagg ctttgtgcag tgctgtccgg acttgcaaac agaaaggacg aacctcacat
     7321 ggtacacttc ggcaacgact gggacaccat tttagacggc gaattcgcca agacctacta
     7381 ccagaacctg cggcagtttc tgttgacgga ataccgcacc cagaccattt atcccggcat
     7441 gtatgatatt ttcaacgccc tgaaatatac cccctattcc caggtgcggg cagtgatcct
     7501 gggacaggat ccctatcaca accccgatca ggctcacggg ctcagctttt ccgtgcggaa
     7561 aggcgtggct ccgccgccgt cgctggtgaa catcttcaag gagatcaagg acgatgtggg
     7621 catcgacaac accggcaagc acggcgaact gaccaactgg gcaaaacacg gcgtgctgct
     7681 gctgaacacc gtgctgaccg tccgtgccgg acaggcgaat tcccacaagg gcaaaggctg
     7741 ggagcagttc accgatgatg tcatccgact gctggatcag cgggaagaac cggtggtgtt
     7801 cctgctctgg ggcaacaacg ccaaggcgaa aaaggccctg atccagcacc cccagcatct
     7861 ggtactgact gccgcacacc ccagccccct gtccgcctac catggcttct tcggctgcaa
     7921 gcatttctcc aaggcaaacg cgttcctgcg ggaacacggg ctgccggaaa ttgactggag
     7981 catctgatat ggcatattcc tatcgctggc agtactgcga catcgatttt ttgcggcact
     8041 gtctgaaaca atacagcctg ctgctggaga agatcccgtc ggcgattctc acggaggaaa
     8101 ccacgggaac gcagatgttc gtcacaggat acctctccga ctgcatcaag cggtgtgatg
     8161 acggattcga cgattacacc atgacggcac ttttgccgga agggacagcc tctgtcacgc
     8221 tgcaaggaaa tgggtggacg attcgccgtc tttccacccg ctaccacggc aagtatgcgg
     8281 cactgcttcc ggaaatagca gcctattact tttccaaagc cggcagacag cgacggcggg
     8341 atacggaaca ggctctggga agccgctgct tcgcattttt cgattatgcg tacaacagaa
     8401 tgttggaaac gcagtttctc cggcacgatt ttcgctgggt gggcggcgaa attccctctg
     8461 tcaatcattt gaaaacgctc tccgacgctg ccgaccttac tgaggcactg gggcaattcc
     8521 ggcgggaaga atatggcagg cgaaagaagc acgatctctc ccatgcaaag ctttgcactt
     8581 tcttggaaac tcagtttgga atactatatt ttgaaaatcg gtctgcttcg tacctgttgc
     8641 agccggtttc cggcggtttt tccgcagatg acggcagcac ggaaagctgg ctgctgtgga
     8701 gatgcaaccg gctgctgggc ggcactgctc tcacggcaga gcgaaaagtg tgccgcctgt
     8761 ttggggctga tgaaaacgaa cggcagcagg tactggcggc atacttctgt ttctgccggc
     8821 aaaaggggat tcccttttcc ggagagatgc agctgtgcca gccggaatac cccgaaccgc
     8881 tggggaaaga tttcctgccg tggaagtggg agtccggcaa ggtcacgccg cagctgctgc
     8941 aaaccatagc cgaccaactg cggcaatgtg ctccggaaca agctgcggac tggtgctggc
     9001 tgctggggac acggctggta tttactgtgc tgccgctggc aaaagaagca ttggcagcac
     9061 tgccccagct ggaacgagat gcctttttcg tacaggcgga gcagttcctg cgggaaacgg
     9121 cgactgcctt aggcgaccag ccgcagccgc tgacggagcc gaacttcgaa aaactgacgg
     9181 tacagtacgg caagcttttg ctagtagatt ccaaggaaaa gctgcggcgg tatgcccgcc
     9241 tgtatcccac attggcatgc accaagtttc cgatacggca gaaaaatggc tatgtctggc
     9301 tgccggagca accagtacgg caatcagcaa aagctgcctc agacggcatc accctgccgg
     9361 aaatccgcag agaactgctg ttgaaacagt tgcaattgcc gccggaaaaa attgctctgg
     9421 aacaggtcta tcccctttgc tatgaggata tgggattttt ctctgaaaat gggagccacc
     9481 gcaaacgtgg tgaagatttt ctgaaagaag caccgcagct gctgcaggat tatgaagcac
     9541 agcatggcaa agttctccgg gaacggtact caccagagga atgggactgg ttccggcagg
     9601 ctgccgatta acccgaatct gccataggaa aaattgataa caagcgatac gattttcccg
     9661 tctactcaaa tacctacaat tatgctataa tataaacaat atcattatag gcaataccgc
     9721 aaattgccgc agaaaggaca acaccatgaa aatcgcatcg ttattttccg aaaaaaccgt
     9781 tttttcattt gaaatttttc cgccgaagaa aacttctccc attgaaactg tctatgacac
     9841 gctggaagcc ttacaggatc tgaagccgga cttcatcagc gtgacctttg gtgccggcgg
     9901 cagcggcaac ggggaaaaca ccctcgccat tgcacggcgg ctgaaagagc actacggcgt
     9961 actgccgatg gcacatctgc cctgtatcca ctataccaag gagcagatcg ataacattct
    10021 gaaacagttt caggaaaacg gcatcgaaaa tgtgctggct ctccggggcg accgcgtaga
    10081 gggcatggag ccggcaatgg acttcgtcca cgctgccgac ctcatcagct atatccgcag
    10141 cttcggcggg ttcgacattg ccggagcctg ctatccggag tgtcaccccg aagctcccaa
    10201 catggacacg gacatccgtc acctgaaaga aaaggtcgat gccggtgcaa gccacctcat
    10261 cacccagctg tttttcgaca acgattcctt ctacgacttc cggtatcggg cagcccgtgc
    10321 tggtgtcaac gtgcccattg aagccggcat tatgccggtg accaaccgga aacagatcga
    10381 gcgtatggtc accatgtgcg gtgccagcct gccccgaaag tttaccaaaa tggtacagcg
    10441 gttcgaggac aatcccgagg ctctgcggga cgcaggcatt gcctatgcca ttgaccagat
    10501 cgtcgatctg gtgaccgacg gcgtagacgg cattcacctg tacaccatga acaatcccta
    10561 tgtggcacgg aagatcagcg aagcggtaaa cggcatcatc aaggcggctc cgaaatgctg
    10621 accctgccgc tggaggcgat cgacaagcag gaggctctcc gctatatggg ctgccccgca
    10681 gaaccggacg aagccctgcg gactgtcgtg gacacctgcg agcagcagct tcgccggacg
    10741 gtacagcccc gcgtagtcta tcgggtactg cccctgtgtc gggaagcggg gacactttcc
    10801 gccggcggca tgcagctaac cggtgaggac attgcacggc atctggcgga atgcgaccgc
    10861 atgctcctca tggcggcaac cctctccgga caggcggatc agctgatcca ccgtgccggc
    10921 atctccgata tgacacgttc gctggcaatg gacgctcttg ccagtgccgg cattgaacag
    10981 gtctgcaacc gtgcggaaga atgctttcgg gagcagttgc cggacactta ttttacatgg
    11041 cggttcagtc ccggatacgg cgatttgccg ctttctctcc agccggagat cctccggctg
    11101 ctggacgcgg aaaagcggct ggggctgaca gtcaccgccg agcacattct cattccgcga
    11161 aaatccgtga ccgcgatcat cggactggca gatcacccgc tgaaaaaagg ggcaagaggc
    11221 tgtgccacct gccgcatgcg ggaaacctgt atgttccgaa agggcggcac gcattgttaa
    11281 aaccactttc aacatggaca cagacacctg tgtccatatt gtgctatctg agcatgtatc
    11341 catacagcat gttccggaca acaaaaggag ccttatctta tggaatacga aatcgtacca
    11401 tataccgaag ccgatgtcga tgccatgatc gagatctgga acgaggtggt caatgacggc
    11461 gtagcattcc cacagttaga cgaactgaac cacaaaaccg gcgtggaatt ctttggaaag
    11521 caggacttcg tcggcgtggc aaaggtgaac ggcgaggttg ccggtctgta tattcttcac
    11581 cccaacaacg tgggcagatg cggacatcac gccaatgcca gctatgccgt ttcctcgaaa
    11641 atgcggggca taaaaatcgg agaagggctg gtgaagcact ccctgaaaac tgccagggag
    11701 ctgggctacc gcattttgat tttcaacgcc gtagtcaagg gcaatgaccg tgcaattcag
    11761 ctgtacacca agctgggatt ccagcgtgtg ggcgtgatcc ccggcggctt tctgctgaaa
    11821 agcggcgtgt atcaggatat ttatgtgtat tatcatgtgc tgtaaaaaac tattgaatca
    11881 ctttctggaa aggacactct cctatgcttt cttccctatt tccgcatcgt aaattcattc
    11941 gcttagacgg cggcatgggt tccatgctgc aaaagggcgg catgcccgta ggtgcactgc
    12001 cggaggtgta caacatcacc gaaccggaac gcatcattgc cgcacagaaa cagttcgtgg
    12061 ctgtggggtc ggacatcatc tacgccaaca cgttcggagc aaaccggcac aaaatggcga
    12121 aatccggcta ttccgtgcag gaactcatct ctgccggcat tgcctgtgcc agaaaagcct
    12181 gtgccggcac ggacaccaag gtggctctgg acatcggtcc gatcggacag cttctggaac
    12241 cgctgggcac actcacattt gaagaagcct acgacatttt ccgcgaagaa gtcctcgccg
    12301 gacaggacgc agacctgatc gtcatcgaaa ccatgaccga cctgtacgaa accaaggcgg
    12361 cactgctggc ggcaaaggaa aactccgaca agccggtgat ctgcaccatg acctttgagc
    12421 cgaatctccg cacctttacc ggctgtaccg tcgcttctgc ggcaatgacg ctggagggac
    12481 tgggagccga tgcagttggc gtgaactgct ccctgggtcc ggacgaactc tttcccattg
    12541 tggaagaaat ggcaaagtgg accacgctgc cgctggtggt aaagccaaac gccggactgc
    12601 cggatccggt caccggtgaa tatctggtag accctgcgga attcgcacag gcagtggtga
    12661 agtttgcaca gctgggcgtt acggtttacg gcggctgctg cggcaccacg ccggaacaca
    12721 tcaaagccgc atacgatctg ctggaagaag cagaaattcc caggcgggaa aacatcgaga
    12781 ttccggcggc agtctgctcc gcttccgaag tggtgctgat cgaccagcca cgcattatcg
    12841 gcgaacgcat caaccccacc ggcaaaaagc ggttcaaggc ggcattgcag gcaaacgaca
    12901 tggactacat cttggatcag gcagtacagc aggtagatgc cggtgccgac ctgctggacg
    12961 taaacgtggg catgccgggc attgacgaaa agggcatgat gatccgcaca gtcaaggcgt
    13021 tgcagtcgat cggcggcaca ccgttgcagc tggactccac cattccggcg gtcatggagg
    13081 cggctctgcg ggtgtacaac ggcaagccca ttgtcaactc cgtcaacggc gaagaagctt
    13141 ccctgcaaaa tatcctgccg ctggtcaaga aatacggtgc cgcagttgtc gggctgacgc
    13201 tggacgaaaa cggcattccc aaaaaggcag ccgaccggtt tgccattgcc aagcggatcc
    13261 tcgaccgtgc cactgcactg gggattccga aacgggacgt gtacattgac tgcctgactc
    13321 tgacggcttc tgccgaacag gacggtgcca gggaaacgct ggaggctctg catcgggtca
    13381 agacggaact ggggctgaaa accgttctgg gtgtgtccaa catctccttt ggtctgccga
    13441 accgtgaggc agtcacccgc acattcctca ccatggctct ggaaaacggg ctggatctgc
    13501 ccattatcaa ccctaacatt gcttctatgg cagaggcagt gcgggcattc cggctgctcc
    13561 gcggctttga cgtgaactgt gccgcctttg tagaagcgta tgccaacatg cccactgcca
    13621 cagcagccgc aacgcctgct gcggcggcga agcctgccgc agaaaaggct ccggaaacgt
    13681 tagacctgcc ctatgccatg tcccacgggc tgaaaaacga gggggcaaaa atcactgccc
    13741 agctgctgga aaccacggat gccatggaag tggtcaacga aattctgatc cccgcactgg
    13801 acagtgccgg aaagctgttt gaacagggaa agatcttcct gccccagttg attcagactg
    13861 ccagcgtggc acaggcggca ttcgaagtca tcaagactac cctgatcgaa cgcaatgcgg
    13921 ctccggtcag ccgcggtgag atcgttctcg ccactgtcaa gggcgatatt catgacatcg
    13981 gcaagaatat tgtacgggtg cttctggaaa attacggcta caccgtgctg gacttggggc
    14041 gtgatgtgcc gtgccagctg gtggcagaca ctgcaaagga acacggcgta aagctggtgg
    14101 gactgtcggc actgatgacc accacgctgg gagccatgga agaaaccatt cagctggtga
    14161 agcagtcttg tccggactgc aagatcgttg tcggcggtgc agttctaact gcggactatg
    14221 ccgagaagat cggggcagac ttctatgcaa aagatgccat ggaaactgtc gccgttgcga
    14281 aagaggttgt tgggtaattt tttccgtgga tcgctgtcct ttcagggact tattccgata
    14341 cattccccaa ccattttgaa aatacaaagg aaacactgca taaagcttgt gcgtaagatg
    14401 cgtagtcaga tttttcggca gat
//
LOCUS       DAWBGR010000048        14243 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1348_length_14243_cov_3.187835, whole genome shotgun sequence.
ACCESSION   DAWBGR010000048 DAWBGR010000000
VERSION     DAWBGR010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14243)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14243)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14243
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1348_length_14243_cov_3.187835"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9226..9235
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10920..10929
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13627..13636
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4021 a         3024 c         3545 g         3623 t
ORIGIN      
        1 gacttgtagc ataaggagga tatgatgagc aaactaatca gtgacaaata caaacaatgg
       61 gaatccgaat gcgaagcccg tttccaaaag ctaaagtcca acgaagagga actgaaccgt
      121 atcttcatcg acatctacgg cttacaggac gagctgacac cggaggtgga ggacagggac
      181 gtgaccgttc gccgtgctga cctcacccgt gagatcaaga gcctgataag ctatgccgtg
      241 ggctgcctgt tcgggcggta ttccctggac gcggacgggc tgtgttacgc cggcggcgag
      301 tgggacgcag gcaaatacaa gactatcatt cccgtgaaag acaacgtgct gcccatctgc
      361 gatgacgact atctggagag cgacctcacc ggaaagatcg tggacttcgt gaaaacggtc
      421 tacggcgagg acaccctgga ggagaacctc aagtttatcg ccgatgccct cggcacaaag
      481 ggtgcaacgc cacgggaggt gatccggaac tatctgctca ccggcttcta cgccgaccac
      541 tgcaaaatct accagaaacg ccccatctac tggctgttca gttccggcaa aaagcatggg
      601 ttcaaggcac tggtctatat gcaccgctgg gaaaagacca ccgttgccac cgtccgcacc
      661 gactacgttc acgagctgca ggaacgctac cggacgcagc tttccatgct gggcgaacag
      721 ctggaacagg cggcaccgtc ggagcgtgtg aagctgaaaa agcggcagga gaaactgacc
      781 gcacagctgg acgagatcaa cgcctatgag gagaaagtcc accacctcgc cgaccgcatg
      841 atcgacatcg atctggatga cggcgtaaag gtgaactatg cgaaatttgc ggaaattctg
      901 gagaagataa aatgacgata gataccataa accgttcgct gaaccagcgg ttcgctgcgg
      961 aactgccgga ctgctatgaa cgccggatca tcttctggtt cgactccgag cgggagtttg
     1021 agggcatgct ggacgagctg gacattcccg gcgtgaagct tctgaagctg accggcgaca
     1081 atttttttgc ggcgaagatg ctcctgtgcg aaacggatac ccggagcaat tatctggtgt
     1141 atgatcccct gacctatcct aagcgggagg acaactggct gcgggatgtc cagctgtaca
     1201 gcgaggaatt ccgtgccgat ctggtttcca tgcagatgga cgagctgcac attccccaga
     1261 ccactcagct acggcgggca atgaaacact acggcaaatt tttcgagagc aaggaacgtg
     1321 cagcgaagct tgtcgccttg gggacgcagt accagaatgc cggacagctg cacatcgaca
     1381 ttatggcggt gctgtgcaat accagacaca ataccgtcaa cggtgtcctg cgtgcgatac
     1441 tctgcgattc gctgtacaac gaggacaaca cctgtctgga gcagatcgaa aagtttggca
     1501 gcatgcaggc actgcaggag atgacggcac ggtatacggg ctatgccgat gagaagtatg
     1561 tgctgtttga cctcgccgca catatcctgc tgacggcgtt ttctgccatc ggggaggagc
     1621 gtgtgctggc tgggctggag aagtatctct ctccggaaaa ccagccggcg tgctatgcct
     1681 tccttgacga gtggaacgcc tcggcggaac aggaaaagct gttcgagacc gccgatgaca
     1741 tcacagagcg gatgcggctt gtggagcgtc tggaaaaggt ggatacggca gtgctgatgc
     1801 gtctggacag cctgccctgt atcgacgagg taatcattgg cagatttatg caggagatcg
     1861 cggagaatgt catcaagaca gaggaaattc tggaaatcgc tgagagccga cgcacatcca
     1921 agtggtatgg cagatatgcc tatttgtatg acgggctgta caatattgcc aaaatgcagg
     1981 agtttcacca tgcccatatt gcaggctttc acttcgggat atatgaggaa ctgtgggatg
     2041 cctactgcaa agagttgtat catatggata catactatcg gcggctgcat atggacttcc
     2101 ggaaaagtct tgtcagtgcg tcgggagcgc tggacgacct gttcaagggc gctgtggtca
     2161 ctgcggagaa cctttacaaa aactggtatc tgtcagaact gaacggcagc tggaatgcac
     2221 tgatccgaga gcttgttgcg gacggatttt ccctgcaggg tattccgcag caaagcgact
     2281 tttatcggag aattgtgcag ccagtcacca atgacagcag agtgtttgtg attatttctg
     2341 atgcattgcg ttatgaggtg gcggcagaac tgacagaaaa gcttttgcgg gaaacgaacg
     2401 gcacggcgaa gctgtcggcg atgcagtcgg ttttcccgag tatcaccaaa ttcggcatgg
     2461 cggcactgct gcctcacaag acactgacgg ttactgatac catgaaagtg ctgtgtgatg
     2521 gagtgtctac agatgggaca gaaaacagag ataagattct aaaagggaaa aatctgaaaa
     2581 actgtgcggt gacatatgaa gcactgctcg ccatgaaaca atcccagcgg cgggaacttg
     2641 tcagcggtgc agaagtggtg tacatttatc acaatatcat tgatgcagtg ggggataagg
     2701 cgaatacaga aaatcaagtt tttgaggcct gtgccgatgc tatggaggaa ctcaaaaatc
     2761 tggtgcgtct gatcgtcaac tctatgagcg gctctaatat tctcattact gctgatcatg
     2821 gcttcctgta ttcctatgaa ccgcttgcag aaagtgaaaa gatcagcacc agtctggtca
     2881 ctgaagatgt tctggaaacg ggcagacggt atctgctcac agacaatgcc ggcaagtcag
     2941 atgttcttat gagcatttct atggaaaagt atgcggaaga gttggtcggt cttacacctt
     3001 gtgaaaatgt ccgtattcag aaaccgggcg gcggcagtaa ttttgttcac ggcggtgtgt
     3061 ctttgcagga gtgctgtgtg ccggtcatca cgttcaagaa tatctcaaaa acctctaaga
     3121 aatttgtaga catcaagaaa gtttctgtca agctgctcag catgacaaga agaatcagca
     3181 acaacatttt ttctcttgac tttatgcaga gcgaggcagt tggcggaaaa acggttcctg
     3241 caacatatga gatctatctg tgtgacgaac tttccaatcc tgtgagcaat gtgcaaacgc
     3301 tgcttgccga taaaaccagc gagccgcagg atcgggtatt ccatgttaga ttcactttga
     3361 aaagcgttgc gtttgatgga agtgcggcgt attatctgaa tattgtggac aaggaaaccg
     3421 gcgagatcat ggagcggaca gaattttccg taaagattgc tttctccaat gattttgatt
     3481 tttaggggaa accgcaatga aaacaagaaa atttaaagta aaaggcggaa tgagaagtcc
     3541 ctgggtgacc atacaaggaa cgactaggcg tgaagtgcgg gcaaaacaaa accgggaagg
     3601 aaatcaggca gtgaaacagc tgatctgggc tggagcgatc ggtgcaaaga tactgttgtc
     3661 ctccaacgga aaaggtcgca tatgtcatta tcgacattcg caaaagcgga atgcaccgga
     3721 ttatcaggca gcacagaaac agctggaaat ggaactgcac gttccaaatc aaaatgcaat
     3781 gggaggaatc atcatggatg aatttgatag gaaagtaatt gcagcatttc ctggcaagtc
     3841 tgttcgtaaa gacttgacat cccttatgaa aaagggcgca aatgttccga cttatgtatt
     3901 ggaatatctg ctgggaatgt actgtgccac agatgatgag gatgcaatca agactggcct
     3961 ggaaaaaatc aagaagattc ttagcgagaa ctatgtgcgt ccggatcaaa gtgattatgt
     4021 aaaatcaaaa atcaaggaaa acgggcagta cactgtgatc gataaggtga ctgtcaggct
     4081 ggatgaatct gaggataaat atgttgcaag ctttaccaat ctggatctgc aggattttga
     4141 ggtgaattct gatctggtaa cacataatga aaagctgttg atcggcggca tctggtgtat
     4201 tatcaagatt gaatatgtag ggcttgaaaa ggaagatgat gaagaggagg aatatgaaga
     4261 agacattttt gacggcaaca aaaagaaacg cggtagaaag cggaaaaaga aaaagtcaaa
     4321 atatgaatcg ccctttacaa tcgcagcact gaaacctata caaatggcaa acctggatct
     4381 tgacgagatc ttcgaggcaa gaaagcaatt taccaaagac gaatggatca cacttctgtt
     4441 acgctctgct ggctatgagc cgaaagagct gagcgaaaaa cagaaactgc actatctgct
     4501 gcggtttgtg ccgtttattc agaaaaacta taaccttgtg gaattgggac cgagaggtac
     4561 cggaaaatcg catgcttaca gcgaactttc tccgtactcg atcctgatga gcagcggtca
     4621 tacaacggta tccaatatgt tttacaatat ggcatctcat cgtgtgggac ttgttggaaa
     4681 ttgggattgt gtggcgttcg atgaggttgg cggcattacc aacacctccg gcgatatgat
     4741 tcagatcatg aaaaactaca tggcgaacgg cagctttgca agaggcagcg attcgatcaa
     4801 ctccgatgcg tctatcgctt ttgagggaaa cacattccgc agtgtagaag atatgctgcg
     4861 aactaccaat ttatttgaac cgtttccgga ggcgttcaac aacgattccg ccttttttga
     4921 tagaatccac gcctatcttc cgggatggga aacgcccaaa ctgagggcaa gcttgtttac
     4981 gaaccgttat ggactgattt ccgactgctt ttctgaattc tgtcatgcca tgcgaaagta
     5041 tgatttcacc aattcatttg gggaatattt cgctttgaac aataatttca atacccgtga
     5101 tgataccgca gtcagacgga ctttttcagg acttgcaaaa ttgatctacc cggacgagcg
     5161 catcgaaaag gaagaggcaa gagaattgtt ggaatatgcg atcgagtgcc gccgcagagt
     5221 caaggaacag ctccggaaaa tgaatccggc agagttcagt gatgtgatgc tggggtatat
     5281 cgatctggac accggagaag aattttctgt ggatctgccg gagatcgcaa gtggttccct
     5341 gattccggag agttttggaa agccgggtta tgtttatgcc attggaagct ctattgatgg
     5401 acatatcggt gtttaccgct ttgaaaataa gctgattgag ggtagcggca aattcaattt
     5461 ccggaatgta gagtgacttg cgggggcgcc gaaaagcgtg cgtgacagtc tgactgcggc
     5521 atttcactat tttgtggaga acagtgagaa gctgatcgac ggcagccctt ccagttttga
     5581 ttacagcttg tattataacg atttgcagaa ccggaatgtg agtgacgaag tatcagtggc
     5641 tgaggttgtt ggcctgtatt cggcactcgc aaatcgccct atattgcctt ctatggtgat
     5701 ttgcggcaga gtggtgatgt ccggtgaaac aatgccggtg attacgatgc tggaagatat
     5761 ttttgttgcg gcagtaaatg caggagcaaa aaaaattctt ctgccggaaa gcagccgaga
     5821 taactatatg ctcctttcag ataaattgaa aagagagatt gcagtgggct tttataaaac
     5881 accgcttgag gcagcaaaga ttgcactggg agttgatctg tgatgaaaaa atatcaatgc
     5941 attgcttgtg gaacggtgaa agaaagcaag gaaagctgta cttgtccggt atgcggatat
     6001 atgatgtttc cgcagccgta tgaacgcagt gaagtaatga tccgtgaaat acgcagcctt
     6061 gcagataaga ccattggtca ggagatcgat gtgagcacat tggattttgg aaagctgagg
     6121 aacgatattg accgttttcc cgattttcag aaaatcaaag tttatgtcac aaagtctgag
     6181 aaaacagaga cattttatcg gcggctgaaa aacagtgcag agcagatgca gcgatatttc
     6241 catgaaacct tctgcaaaaa ctattcgtca aaatgcaagg aactggaaac gctgtcagag
     6301 gagacggctg tatatttgca gcgtgtgctt ttggaacttg gaatagaagc agaaccggaa
     6361 aaagcagctt ttccagttgt cacagtgcag tatacggagt gtgctaatca tgatctcatc
     6421 gggattgccg accagcttct cgaaaagatc gatcagatgg cagataagat ctatcagttt
     6481 atacgggcaa acaacattta cggacgtgcc tatgatgcgg aagtcaaaac attccatgtt
     6541 ccggaaaaaa agcaagagat caactggaag gatatgatgg cagagcgtat ctctgcctgt
     6601 gacaaagttc tggagaagaa atatatcatt gatatttttg aggatggcag tacagaactg
     6661 accgccatgg tgaaagtact gtgggatgcg gtatttgtac tcttagctgc tccggtgaaa
     6721 aaaagaaaat atttctacac ctttgacagc gataaagttt atgaagaaat gtctgcgaaa
     6781 gcgtgtaata aacggttatc ctcgatcttt gcagggcagt ttcatgctgc cgctgaaatc
     6841 atcgatgcag aagattttct gaaagacaag tcggaagatg aactcttcga actctatcag
     6901 aaaatgcttg atcttgacat gtatcactat atgaccggaa caaaaggcta tcttgtagtc
     6961 ggcagctgtg aaaaaaagct ggaatcactt attggactgg ataccataaa atcaagcatc
     7021 cgaaaaatca aggcatatgc gatagcaaat cagggaaatg cgaaacccaa tctgcatatg
     7081 tgctttctgg gaaatccggg aacgggcaaa acggaagttg cccggatcat tgcagggata
     7141 cttcatgaaa atggccttct ccgcacagac agggtggtcg aaacagaccg cagcggtctt
     7201 gttgcaggct atgtcggaca gacggcaatg aaaaccgcag ccgtcattga agaggcaatg
     7261 ggcggtgtgc tgtttattga tgaggcgtat tctcttgtgc agggagacac aggcggtgac
     7321 tatggccatg aggcagtgtc aacgctgata aaggcaatgg aagactatcg gggtgaattc
     7381 tgcgtaattc ttgcaggata taaaaaccag atgcatgaaa tgcttgcgtc taacccggga
     7441 tttcagtcta ggatccaatt tacgttggat tttccaaatt acaatcggga cgaattgggg
     7501 cagatcgctg cactgatgct gaaatccgga ggatatactg caagtaatgc ggcaaaggac
     7561 agaatgctgg atatcacaga ttatatccga aaagaaccaa actttgcaaa tgcccgtgag
     7621 atgcggaata tcattgagca ggtcattatg tgtcagaatg tacgctgtgc agggactgcg
     7681 gataaaaccc tggaacttgt ggacgtgaac aattatatca aggaaaatca tgtcaatctt
     7741 cccacaagcg gcgagggcac agaaaggaag atcctgacgg cagaggatga gttggaacag
     7801 ctggtgggac tgcgctctgt gaaacgcatg gtaaagaaaa tcaaagcata tgtcaagcgc
     7861 aataaagacg attcggatat gaatctgcat atgtgttttt gcggcaatcc cggcacggga
     7921 aagacggagg ttgcccggat cttatctcgg attctgtatg aagccggtgt gctgccggag
     7981 gccaaactca cggaaaccga ttcctccggt ctcatcagta aatatgttgg cgagacagca
     8041 tcaaaaacgc tgaataagat caatgatgcc atgggcggtg tcttgttcat tgacgaagca
     8101 tatagtctta cccaaagtac agacaatgga acagccggtt atggtgatga ggcggttgca
     8161 gttcttttga aacaaatgga ggatcggcgc ggacagttct gcgtgattct tgccggttat
     8221 aaagatgaaa tgggtgcatt tatcagtacg aatccgggat tttcctcaag aatacagttt
     8281 tcgcttgagt ttcctgatta tacgcaggaa gagctttccg agatcgcaca gcgttttctg
     8341 caacgaaaaa aatataccat agatgacgat gcacttgcac ttgtgcttga tattgtggaa
     8401 tatgacaggc taagaccaaa ctttgcgaat gcccgtacag ttcggaatat tctcgaacag
     8461 gtgatcatga accagaatct tcggacggat gaggaaggca tcacagattc tgcgatcata
     8521 cgatcggatg tgcaggatta tctggaggat gaaaacattg acttaaacag tacggcagat
     8581 caaactcgaa gaattggatt ttgaatgtgg tgaaaatggg atggaggatt tcttattatc
     8641 tcattgtgtc tcctggatac agcggagaaa gcaagtataa agccattctt gagcaggcaa
     8701 aaaggtcata cagtagaagt gatattctat gatgaacttg ttaaatacaa ctgtgattgc
     8761 taataaacat ttgatctact gcgtaagcgt ttatgccgcg aaaattactt gacaatgagt
     8821 atgactgtgg taggctaatt gccgagcaga agcgctattg cgtcattttc tccttctgcc
     8881 caaagccaca gtcatagagg ctcattgtta gcaaaccgcg gaataaatgc gtctaattcg
     8941 tagttaaagt ggctcaaaat ttaaatatca tttcatgtga ttttggctca cttttttatt
     9001 agcgaacata gctggagcat ccaggatcac tttacctatc acaaaacagc ccttggaacg
     9061 gcaagcttat tgctgttcca aggtctgttt gttatcctct aaaaacgact ttctatgaaa
     9121 ttctcaaagg atctgactaa attcgattac ttcgcagttc ggccctgtaa tcttgaagga
     9181 ccttgaagaa ctttacgcca tgctcccaga agggcagaaa atgaannnnn nnnnnatatc
     9241 tttctggttg ttcagtccaa gtgcacagat ctcttcatag gcggcttcga tgtccttgac
     9301 atcgattgcc agatgatccc atgctcctgc ctgcatcgat gcctgatgat tctcatagat
     9361 ctccagcatc aggtttccca actgcaaaaa tgcaactgct tcgccggctt cttcgttata
     9421 ggtgcgaagc gccacgctga atccaagctt ttcataaaaa gcaatggttt cctgcaaatc
     9481 gttggtcggg aatcctatgt gctggaatcc ggtcgcattg tcctgcaaat tcatgcttat
     9541 aacctctctt cttttaattg ctccatgacc tgtgccatat tcggcattgc cggaatcgcc
     9601 ccgtgtttct ggacgcaaac gctcgccaca gcatttccga aatctgcaaa ctctgctgct
     9661 tcttctgttg tgacagcttc cggtgctttt ccgcagcggc tgaactgaaa cagaaatccg
     9721 ccccagaatg catcgcctgc accagtcgca tctgccgcct tgttccggaa accctttacc
     9781 agctgtccgc cctgtctggt ttggacgtag gcacctgttt ttccaagtgt tacgaccacg
     9841 atttttacgc cgccctgcat caggacttcc gcagctttct gatagttgct ctcaccagtg
     9901 agcagcatgg tttcttcctc tgagatcttc atcagatctg catgcggcac catgttccgc
     9961 atttgctttt tggcaagttc ctccgatttc cagagggaag cacgataatt cggatcgtaa
    10021 gagatcacac agcctgcttc tttcgctttc tgcaccgcaa acagggtggt gctgcgagct
    10081 ggctcatcgg tcagcgaaat cgagccgatg tgcagaatcc cgctgctgcg gatcagttcc
    10141 agcggaacgt cacccttttc caacatcttg tctgcaccgt gattccgtgc aaaggagaac
    10201 tcccgctctc cgtccggctt gacgtccaca aatgccagtg ttgtgaaata atcattgctc
    10261 agcgtaaaac cagtgctgtc aattccacac tgttccagcg tttccctgag aaatctgccg
    10321 tgcatatcat ttccggcttt gccgataaag gcggttcttg cccccagctt tgccatagca
    10381 gccatgacgt ttgccggagc accgccggca ttttgtgcaa agacacgctg tccctgttca
    10441 tttctgccct gcatggtgaa gtcaatcagc atttctccca gtgcacacgc cagaaatgtg
    10501 gttttctgaa tcattggtcc ttccggacac aaaattccca aatgttccag ctcttccaaa
    10561 aatggtgtgc agacgttcgg gtcgtcggag tatacctgca aaatgccgga tattacgatt
    10621 acagcaagaa caacgactgg aatgctgatt ctgaaaaaca gatagtgatc ggtgtttatc
    10681 gtctgacgat gaagtccaac agcgataact tccgccagtc ctcaattcaa ggggttatga
    10741 agcgtataaa agctaaaggt gcaacagtta tcatctatga accaacgtta gaagacggcg
    10801 aaacattctt cggaagcgtc gttgtcaacg accttgcaaa attcaaggaa atgtctgatg
    10861 cgattatcgc aaacagatat gatccatgtc ttgatgatgt agcagaaaag gtctacaccn
    10921 nnnnnnnnna gagattaaaa ggagtaaaac atgtgcggaa ttgttgggtt tacaggaaaa
    10981 aatgaagcag caccgattct tctggacggt ctgtcaaaac tggaatacag aggttatgat
    11041 tcagccggta ttgctgtcag aaacggcgat gcagagcctg aaatcgtcaa agcaaaggga
    11101 aagctgaaag tcctaaagga aatgaccaat gacggcaagg cagtcaaagg aagttgtggt
    11161 attggtcata ctcgctgggc aacgcatggt gaaccgtctg ccacgaatgc tcacccgcat
    11221 tgcagcgatg acgaaaatgt tattgcggta cataacggca ttatcgagaa ctatcaggaa
    11281 cttcgtgaaa agctggtcaa aaagggctat accttcaagt cacagactga tacagaagtg
    11341 gcagttaagc tgatcgacta ttactacaag aagtacctcg gaacacctgt agatgctctg
    11401 aatcacgctc ttgtaagaat ccgtggctct tacgcacttg cagtcatgtt caaggactat
    11461 cccggtgaga tctacgctgc cagaaaagac agtcctatga ttatcggtgt gacaggcggc
    11521 gaaagctatc ttgcatctga cgttcctgct atactgaaat ataccagaaa tgtctactat
    11581 atcggcaatc ttgagcttgc aaagctgacc aagggagaag taaccttcta taaccttgac
    11641 ggtgaagaga ttcagaagga aatgaaaacc atcgactggg acgcagaggc ggcagaaaaa
    11701 agcggatatg agcatttcat gttaaaggag atacatgaac agccgaaagt agtcagggat
    11761 accatcaact ctgtcctgaa ggacggaaat atcaattttg agagcctgac agatgaagaa
    11821 atgcagaaca ttcagcaggt atatatcatt gcctgtggtt ctgcatatca tgtaggaatg
    11881 gcggcacagt atgttctgga aaagctgacc tctctgtctg tccgtgtgga gctggcatcg
    11941 gaattccgat acagagaaat gcagcttgca aaaaacagtc ttgccattat catcagccag
    12001 tcaggagaaa ccgcagacag tctcgcagca cttcgtcttt gcaaagaaaa gggcgtaaaa
    12061 actcttggta ttgtcaacgt agttggttct tctattgcaa gagaagcgga taacgtgttc
    12121 tatactcttg caggtcctga aatttccgtt gccacaacca aggcatacag ctgtcagctt
    12181 gttgcagcct acctgcttgc catgcagttt gccaaggcaa gaggagagat cacagaagag
    12241 agattcgcag agcttatcgc tgagatcaat acgatccctg aaaagatcga aaagattctg
    12301 gaggataaag agcgtttgca atggtttgct tccaaggtag tgaatatcaa ggacgcattc
    12361 tttattggac gtggcatcga ctacgcaatc ggtatggaag gcagtctgaa actgaaagag
    12421 atcagctatg ttcactcgga ggcatatgca gcaggcgaac tgaaacacgg accgatcagc
    12481 ctgatcgagg acggtacagt ggtattcagc gtagtaacac aatctgcact ctatgaaaaa
    12541 actatcagta atatggtaga agtcaagagc cgtggtgcaa agctgttcgg actcacgaca
    12601 tacggcaatt ataacatgga agatgtcgca gactttacgg tgtatatccc gaaaatcgac
    12661 aacctttttg caggcagtct tgctgtaatt ccgctgcaat tgctgtctta ctatgtgtcc
    12721 gtaggcaagg gatttgacgt ggataagccg agaaaccttg ccaagagtgt gaccgttgag
    12781 taagcggaaa aagctgctaa ttgcagtctt tttgatatac atatttattg ttctatggct
    12841 gacactgatc gacagacagt ggggcgaacg cagagcaatg cttatgccct tttgggaaat
    12901 gcgggaactc ataacaacag atagaaaact ttactggtca cagcagatcg ggtgcaatat
    12961 tcttatgctg atgccgatgg gattcttgtt gccatgcctg caccaaaagt ttcggaaact
    13021 gaggattatt ttttttgcag cactggcgtt ttcagtattc atcgaagcta cacaatactt
    13081 tactggcaga ggtctttgtg agtttgacga tgtgtttcac aatacgctgg gtgcttgtat
    13141 aggatttatt atatggaaaa agttaagcca ttgaggaaat caaacctcaa tggctttttg
    13201 tcatttgtgt ttatattcgt tatactgttc ttctgtgatg ttggcggctt tcaaggcgga
    13261 ttctaaatca aagcccatgt ttgaaactaa gttgtcaact atttcagctt tacctcgcat
    13321 tttgccctct ttaatacctt tttaaagcct cttctttcag aattcgtaag agcagaacgt
    13381 tcatccatga ttgctttttc acgcatttca gcttcgtatc ttgttttttc atcagcactc
    13441 atttttctga tagtgaagat gatgtcatgg atatccttta catttgttgc agattcaatc
    13501 atttcaagat caccctccgt ttctgcattg atcaaatcga gccaaacctc tacgggtttg
    13561 ttctttctgg tatttttaag ctttttcagt tcaaagaaaa caatcgaaaa tttgtctgta
    13621 agaatgnnnn nnnnnngtat ttggattcct caagagcttt tgccatgtgg ttagtattca
    13681 aaccgatcaa taccgccgga ttttactctg catcttttct gacagaaaca tatgccaagt
    13741 ttggcgatat tttcgtattg ttcatctctt aaagatgctt cataatcctt atcttcgatc
    13801 tgctgtaatg cctcatcaca ggcattaagc atttggttga atttatcaac gattttgaat
    13861 tccagtatgg cagcatagtg ccatttggaa cgttgcttca ctaccaaatc aaaacgcccg
    13921 tttcccgact ctctgtttga aacgactaca tatccattaa atcctgaaac aagtcctgcg
    13981 acaaacccat gatagaaatt ttctttttca tcaaaacaac tgatcgtatc tgtcatccat
    14041 gtacagagaa gatcttctaa tttttctgca tctgcattca ggatggcttc aagaatatcc
    14101 tttctgctgt cagcattgat ttttttctcg aaccaagctc gaattgtatt tttgtaaatg
    14161 cttttgattt cagtgtttgg aattaccatt tcataataag tttcatcacc gattgtttcg
    14221 taagaaatta cttttaggta acc
//
LOCUS       DAWBGR010000049        14066 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1369_length_14066_cov_4.953322, whole genome shotgun sequence.
ACCESSION   DAWBGR010000049 DAWBGR010000000
VERSION     DAWBGR010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14066)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14066)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14066
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1369_length_14066_cov_4.953322"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3713..3722
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11423..11432
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3189 a         3914 c         3448 g         3495 t
ORIGIN      
        1 accggcacac cctcaatttc cgcgggcacc tcgattgtcg ttgcattctt gtcacagcca
       61 gtaatggtaa cagttttcgt ttcatcgctc acaatttcat acgtcaggtt gtcgtaagtg
      121 agatcttccg cacttgccac aacagacacc tgcggtagat gcacctgtgc caccggcact
      181 gcaccgacgc acatggtcac agccgtcaaa gctgccaaaa aagttctgat ttttttcatt
      241 gctttttact cctttcacaa atctgtcatt atacgaacgt acaactttcc ataacacacg
      301 ctagcatttc ctattatacc gcaaaaaaaa aaaaaaaaaa taaacaactt tttgagaaca
      361 ttctataaac tgtgaaatgc aacaacattc caaacacaaa aaagcctgta ttccgaaaaa
      421 aagaatacag gctttctgcc tctcgcgaat gcaagcatta tttcaccaca tctgcccatg
      481 tactgcggac acccgcacca ttcagggcga tatagtacaa caaatagtac acgtctgtgg
      541 aatcgatgct gccgctttcg tcgatgtccg cagcttgcat ctggtcagcg gtgagattgc
      601 cggctctgcc cacagcggtg tatgccacgt agagcattgc cgcgaacacg tcactgctgt
      661 cgatcttgcc gtcgccgtca acgtcgcctt ttatgaacgg cacaaccggt tctgtcgcag
      721 tggtgactgt ggtagtcgcg gtagtttccg tggcagttgt ggttcctgcc ggcgtggtag
      781 tggacgcgga cgattccccg ttgaaatgga tacgcacttt cagaatcttg tccagtgtcg
      841 tgcagaaccg aatcttgttc cagtcctcct ccgagccggc atagtacaca tcggtcagac
      901 tgtcacagcc ggcgaaactc tcaaatgctc cgccgatctg tgtcacgctt ttgggaatga
      961 caacttcttt caggttggtg cagtaggaaa acgcgttgga gccgattcgc tgtgtgccgt
     1021 cggggatttc cacccgcgtg aagccgtcac agctgaaaaa ggtgctttcc ccaatggtgg
     1081 tcaggttctt aggcagggtg atttccggca gctttttgca ctggcggaat accgtctgcc
     1141 ccagttccgt aatttcatcg gaaagccgca cccgttccag acttgtacag ccataaaaga
     1201 cgctgtagcc cagtgtatct gtcgcatccg gcagcagtgc ctcctgtagt ccggaacagc
     1261 cggaaaacgc ttcattgccg atacttttca gcttttccgg aaatgccact gccgtgaggg
     1321 acgagcagtt ccggaaagca ttgccgccga tctgtttcag attttgagga agcgtgacgt
     1381 gctgcaaatc tgtacagccg gagaatgtgg actccccgat ctgggtaaca ctgtccggaa
     1441 tggtcactgc ctgtagggaa acgcagtcca gaaacatgct gtttcccagt tccgtcaagc
     1501 cctccgccag tgtcacttcg gcaagtctgc ggcagtcgca gaacatgttt ttcccgagtg
     1561 tcagcacgtt tccggaaact gccgcctgct ccagtgcggt gcagtgggca aaagcgtagt
     1621 cgccgacttc tgtgacctgc tccggcaggc tgatgttctg caaaccggaa cagttttgaa
     1681 atgccccgta gccaagcgtg gtcaggcttt ccggcagggt gagcgtttcc agtttggggc
     1741 agtcttcaaa ggcacagctt tggatctcgg tcatctttgc cggcagtttg acctgataca
     1801 gacgggaaca gccggaaaat gtggagccgc ccagcgtctg cacctgttcc ggaatggcaa
     1861 ccgttttcaa cgcagtgcag ttctggaaaa cgctgccgcc cattttctgc aagttggcgg
     1921 aaagctgcac ctctgccaga ctttcacagc cggagaacac gctgctcccc aatgtttcca
     1981 cactgtcccc caacactgcc ttttccagat tggtgcagcc ggaaaaagcg gagttgctga
     2041 cagactgtgt gcctgacgga actgtgatct cctgcaagtt gatacagccg gaaaatgcct
     2101 gtgagccgat gctctgaacg ctgtctggca aggaaacggt ctgcaacttc tgctgcttga
     2161 gaaaggcact gtccccgacc gccgttaccg gactgccgtc gatttctgcc ggaatctcca
     2221 gtgctgtcac acctgccgca cagccagtga tctcggcgtt tccgttgtgc agcacgtagg
     2281 tcagatcgcc gtaggtctgt tctgccgccg atgccgacag ggtgtttttc gatacccaca
     2341 gtgccattac ccgcatgatt gctccgcaaa ccacccctgc gaccagcagc accagaagct
     2401 ttttccacca tttcatgttg attttcccct tttctgtgga attctttctt atctcagcac
     2461 atcgtcccac gaactatgaa ttcccgcacc gttcagggca acataataca ttaaataata
     2521 aacatctgta gaatccacag tgccgttgcc gtcaatgtct gcggcggtca gctgtccgtc
     2581 agtaagcgtt ccgcctacgc cggcaccgac acttgccaca tgcagcagcg tgctgaacac
     2641 gtcggagctg tcaatggtgt cgtcgcactc cacgtcccct ttgacaatgt ctttcacttc
     2701 cacattcagc gtgtaggtct tgccgcccag atctgcggta agtgttgcct tgccataatc
     2761 tttcacaaca gcggtattgt tcccctcaat gctcaccacg ctgggtgcag aggaactcca
     2821 cttggcatac tccaccggaa agccgctgtg cccattataa gaagcggtca gctgcaaggt
     2881 tttccggtca ccataataca ggtacttgct ctggttagag gaaatcgtca ccgcactcgc
     2941 cggcacttcc agcgtctgga cacaatcacc gtaggtgaac tggaacgttt cactgttgtt
     3001 ggtgccgctg aactccgcac aggaagtgct gtagtactgt gcggtactgg taccaaaagc
     3061 tcccatgccg acatagcggt tgctggggga gatcatactg gtatagtgtc ccgtcacgcc
     3121 gccggtctgg ttgaggtaat cgtccttttc gttgtaccac aattccacac cggtcgtcat
     3181 atccagatcc cagctccacc acgcaaggca ttcaccgccg gaactgtagc cccctggggt
     3241 ctgaatctga aaacaattgg aaccattggt gcgttcgtga gcaatcgtct gggaactctc
     3301 tgccgcacga atccgtgcca gatattccag tccggcagac cactccatgg ggacgtagtc
     3361 gtttttggtc agctttttgc cgttcaccgg attgggatag ccattgctgc aagcctccca
     3421 gcggtactgg ttcaccagtt tcagagccgc ctgtatatcg ttggcataag tgccctccat
     3481 tccgatgaga acattgcctg ctgacggcac tgtaacatct gtgtccagaa ccggctttgc
     3541 agatgcggcg gaaaccgtca tggaaactgc cccgccggaa accagcattg caccggcaag
     3601 caccgccgca gtcagtctgg aaaaaatttt gcgtgtcttt tgcatgaaaa cgaccccttt
     3661 ctgatatggg caacaccgca caagggcaat accgcacaaa gattgtgcga cannnnnnnn
     3721 nnctctttgt gcggtattgc cctagctttg ttcagtgttg cctgtagaag atttcttcca
     3781 tatttttatt ttatatcttt tatcatacca tatttctgtg aatgtagttt acaacgcata
     3841 aaaatatttc atgaattgca gtgagaaaat taaaaaggtc gctgtaccgt tttcacaata
     3901 cagcgacctt cttccagaac tatcgtgatt ccagcgttgt ggtttccgtt gcaacagcgg
     3961 ctccggactg aaactgggta aatgtcaggg tggtatcaaa attttccatg gtaaagccgg
     4021 acaaatcccc gttttccgtc agtgtcaaag tgtagggatt gccctccaag tccccctcca
     4081 ccgacacgca gccgtcctgc tccttgccgg tgatctcctc ctgctgtgcc agttcatcta
     4141 ccacctgaat catctggctc agcaccgact tcgccggcat ggcggactgg ggcacggaaa
     4201 agcccagccc cttatatgac gctgtcactt ccccgccgga aaagtccagc accacaccgg
     4261 caagcgtagc cggctcggca aacgacacac tccatgctcc gtcacccaag cgggacagcg
     4321 tgccggctgc ggtccagtcc tccattgtca tggtcatttc cgtggtaaaa ctgccgccca
     4381 gcttttgagc agcagtggct tcgccgccgg ctttggcaca gccggtacag cagatcccgc
     4441 cggcgagcag caaactcaag acagcactgt aatttcgttt catgcaattt cctcccaatc
     4501 agagcctttc ggacaatatt gtgcagtgtt gcacattttg ggcagagaaa gggcggatac
     4561 atttcaccgt tcctgttcca gcttgaccag cacctgcggc agatatgcca gcaagtcccg
     4621 cggcagcatt gcctgctggg acagtgctgc cgccgccgca tctccggcaa ggccgtggag
     4681 atacacccct gcacaggctg cctcccaaga cggaatcccc tgtgcggcaa aggcggcgat
     4741 cagtccggcg agaacgtccc cgctgccgcc gcggctcatg cccggattgc cggtggggtt
     4801 ctgtgccaga cggctcccct gtgccaccag tgtgccggct cccttgagtg ccagcgtcac
     4861 cggataggcg gctgcaaact gcgttgccgt ttccacacgg ttttcctgta tctgcggaat
     4921 cgaacgtccg gtcagccgtg ccatttcgcc ggggtgcggt gtcaggatcc agtccttccc
     4981 tgtccttctg cttaactcta tgcaggaaac cgcacagttt agaccatctg cgtccagcac
     5041 caccggacac accgcctgtt ccagcaccca gtgcaccagc cgacgggttt cttcggtcac
     5101 gcccagcccg cagccgagca gcaccgcatt ggcacgggaa agctgctttt ccagcgtttt
     5161 ccggttctgg tcgctgctgc ggagaaatcc ggcttcgtcc gtttccagcg gcagccacat
     5221 tgcttccggt gctgccgccg ccagccgcat gggattttcc ggagccgtgg caactgtcac
     5281 cagtccggca ccgccccgca gggctgcctg tgtggaaagc aggcaggctc cgggcatatt
     5341 ccggcttccg gtaatgcaga gcagccgccc gaaatctcct ttctgggcat cggcttttcg
     5401 tttgggtatc atctgacaga tttcctgcca gttcaattgg tgcggcatca tgaaatactc
     5461 ctttcgcaat gtgccggaat ccgacagcac ttctgccgga ttgacaaaaa aatttctgtg
     5521 tgttatgata gagtgtcggc ttcttccgtc agggaggagg tgataatttg ctatactata
     5581 tcattatcgc aggtatctcc gttggagtag acattgtgct gtacaaagtc tacaaatggc
     5641 tgaacggaga gtaagccgac accagcctac ttatttccgc ctttttatag ataaggcgaa
     5701 agcccccaga gttgcagatc tgggggcttt ctttttagtg attacttgca attatattat
     5761 ttcaatgtaa attattatag cataaactac gacaattgtc aagtgtacag aaatgctttt
     5821 aaaaatggaa cgtagaccaa caaatatgcc tacttcattt gccttattga cagacaaggt
     5881 gaaagcccct agagttgcag ctccagaggc tttctgttgg taataatttg ctactatatc
     5941 attgcaagat tattatatca caaccagctt gctttgtcaa gtgtgcaaaa acaccttttg
     6001 cagcaccgcc taaaaatcgc acacgacctg cactgccaaa ttgctggggt cagatgttcc
     6061 gtctactctg tagacctcta acgcctgctt caaatctgcc agtatttgtg atctgatatt
     6121 cccctgtacg tatctcaagg gattttccca tgaggtttgg aaactccaga ttcgcgtatt
     6181 cggcagctgg ttttcctttc tcagcggcaa aatcatctct tgatctttcc agtgaaaaaa
     6241 gaacaactcc tcattcgggt aatcccgatg cacgccgatt gcaaacaaaa acacatcttt
     6301 tcccgcatct atgatccact ctctcggctt gagaatccct aaaacgggag ataacggatc
     6361 ggggatctgc ttcgctgcaa actgttcccg ttgttccgcc gtcaatgctt caatttcaaa
     6421 tgccatgccg tacctcctgc tgtttcattt tcgaccattt tattataccc tatatttcac
     6481 gaaaaagcaa atatcccgaa caatgttttt ataattcata atttatttac ttgatatttg
     6541 catgtttcta tgctataata aatttgtttg acacttccat atttttacgt gcgatttgtc
     6601 aaataatttt ggggacagga ggtaattgac acatgaatca gaaaaacgga aaggcagttg
     6661 cggctctggt actgggaatc gtttccctgc tgggactctg tatcccgctt gcatgtatca
     6721 tctgcggcat cattgccatt attctggcag tcatggcaaa gaaagagggt tccaccgacg
     6781 gcaaacagaa agccggcatg atcttgggta tcatcggcat tgttatcagc atcattatgt
     6841 ggatcgtcaa tgcggtgatt ctggcaggaa gcggcattct ggatcagatt tccagcagca
     6901 tatcctaagt taacatgatg acaaaagcaa aagcgtgcag aagtatctga acttctgcac
     6961 gcttttttat gttcctgttt cagggaatat tgatactatc atgatatgcg aataagggtt
     7021 ttcaacaacg aacgaagaaa aatcgcccta aaatgcacat gtcagacttc gtgtcaacac
     7081 gccccaggtt actgcatctt catagaaatg atcgcagaca gtacggtcaa aaagcctgcc
     7141 actgcggcaa caattgccgg cagggttccg ctttttccca ccatcaggct ggcaaaacca
     7201 gtcagcacgg caatgccgcc cagaatggct gccaccagtg tagacagcca gagcagcgat
     7261 gctacaccaa caacggcaag ccccagtgcc accagtccca gcattaacgc agtcgcttct
     7321 ctgggagtac gctcctgctt ttcctttttc ggttttggtt gtttttccgt tttttctttc
     7381 atggaaatca cctcaaatag aaagccctgc tcttatgctt cccgcagcac agcgtccatg
     7441 gcagccagtg ctttcagtac ccgcttcatg gctgcatctg cctgttcgtc agtcagtgtg
     7501 ccctcgtggg aacgcagccg cagagagaac gatacgctct ttttgccggc agcgatctgg
     7561 tcgcccttgt atacgtcgaa caaggttact tcttccagca gctttccggc agcctgtgca
     7621 atggcttctt ccattttgcc aaccggcagt gcatcatcac acagcaggct caggtcacgg
     7681 gtgattgccg ggaacttcgg cagcggctga taggtcactt cagtttctgc cattgcagcc
     7741 atttcctgta ccagcagttt tgccacatag gttctggtgc cgatgccgta ggtcttctgc
     7801 accgtggggt gcagttcgcc cagatagcct accggtgttt ctccgcagag aatgacagca
     7861 cttctgccgg ggtgcagtgc acagcactcc tcaaaaacgc cctccacttc cgacggacgc
     7921 acataggtgt acttcggcag acgcagttcc ttcagcaggg catctactgc acccttgagt
     7981 gcaaagaagt ctgcatcgcc gccgtacata ccgatcgtca ggcggttggg ttcttccggc
     8041 agagtgtccg gaccagtcgg caggtattcc ttgccgatct cgtacagtgc cacagcggca
     8101 ttccggttct tatagttggt tgccagtacg tccagcatgc agggcagtgt ggtcgtccgc
     8161 atgatactgg tatcttcgcc cagcgggttg gtaatggtga tcgtctgacg cagcttgctg
     8221 tctgccggca gaccgatgcg gtcgaagcac ttcggagaag tgaacgaata agtcagcgta
     8281 ccgtaatagc ccagtcccac catcgtctgt tctgcctttc gcaggaactg ctgctgcggg
     8341 gtcagctgtg cttctgccac accgcgaata acggtagacg ggatattgtt atagccgtaa
     8401 attcttgcca cttcttcggc aatatctgcc ggacgctcca gatcgatccg gaagctgggg
     8461 gaaatgcaaa cgtcgccgtc cacatgaatc tccagtgctt ccagtgtctt gaccatatcc
     8521 tcacgggaaa tttccgtgcc caggaaccgg ttgatccaat ccggatcaaa gggaatctgc
     8581 accggttctg cggtgcggtt gtccacttca atgaactctg ccaccggttc gccggcaccc
     8641 agcagttcta ccagttccat tgcccgtttc agtgtccgcg ggcagccgtc cggatccagt
     8701 cccttttcat agcgtgcaga agcatcggta cgcataccca gcttcttagc agtccggcga
     8761 acctgtaccg gttcgaaata agccgactca aagataacgg tattggtgtc gtccataatg
     8821 ccgctgtatt cgccgcccat gacaccggca acggcaatgg gcttttctgc atcggcaatg
     8881 atcagcattt ccggagaaag ctgccgttcc tgtccgtcca gtgtggtaat ggtttcgccg
     8941 tccttggcat tgcggacaac gatcttgccg tccttgacat accgctggtc gaacgcgtgc
     9001 atcggctgac catattccag catgacatag ttggtgatgt ccaccagatt attgatcgga
     9061 cgcacgccgc tggcacggag ccgttcccgc atccagcggg gagagggacc gatcttgaca
     9121 ttcttcacca tgccggcaat gtaccgcggg cagagtgcag cattttcaac ggataccgac
     9181 aggctgtcgc taagcttgtc tgcggagccg tggaattccg gtgctttcag gttcatgggc
     9241 ttgccgaaag tcacggcagc ttctcttgcc agaccaacta ccgacaggca gtccggacgg
     9301 ttagaggtga tctcgaattc gacagaagta tcgttcagac cgatcgcctc gtgaatgtcc
     9361 tgtcccaccg tgcagtcctc ctcgatgagg aaaatgccgt cttcaatggc ataggggaag
     9421 tcatgcttgg tcaggccaag ttcgcccaga gagcacagca tgccgttgct ctctacgccg
     9481 cggagcttgc cctttttgat gcggacaccg ccgggcagtt ctgcaccgat cattgccacc
     9541 ggcaccagtg caccctctac gatattctgg gcaccggtca cgatctggat cggagaatcg
     9601 ccgccgacat cgacctgaca cacctgtaag tgatcggaat tttcgtgggg cgtaatggac
     9661 accagcttgc cgacgatcac gttcttgaca acttcgccct ctgtttcata tgtttccact
     9721 ttggagccac tcatggtcat gccggcacag aattccttga cggaaacgcc gcagtccacg
     9781 tagtcagcca gccacttcaa agataaattc atgtttttct ccctcccgat cagaactgtt
     9841 ccaagaaccg ataatcgttc tcatagagca gacgcatgtc accgatgtta taacgacgca
     9901 tagcaatacg ttccagaccc agaccgaacg caaagccgga ataaacactg ctgtcaatgc
     9961 cgcagccctc cagcactttc gggtgcacca tgccggcacc cagcagttcg atatagccct
    10021 cgcccttgca catgcggcag cccttgccgt gacactggaa gcacatcacg tcaacttctg
    10081 cactgggttc ggtgaacggg aagtgatgcg gacgcagacg gatcttgcag tcatcgccgt
    10141 acagccgctg catcagcagc tccagtgtac ccttgagatc tgccatggtg atacccttgt
    10201 ctacgaccag tccctcgatc tggtggaaca gcggggaatg tgttgcgtcc actgcgtccg
    10261 aacggtaaac ccgtcccgga gagatcacgc ggatcggcgg cttctggttt tccatgacgt
    10321 gtacctgcac cgaagaagtc tgggtacgga gcagaatgtt atctgtaata tagaaggtat
    10381 cctgcgtatc ccgtgccgga tgatccggcg gcaggttcag ggcttcaaag ttgtagtagt
    10441 cgtattccac ttccggaccg tctgccacag aaaagcccat gccgaggaag atttcccgaa
    10501 tctcgttttc cacgatggtc agcgggtgca tgctgcccac tttctgcttt ttgccgggga
    10561 gcgtaatatc cagtgtttct gcttccagct tgtgtgccag cattgcctcc gccagcttct
    10621 tctgcttgtc tgccagtgcc gtttcaatat ctgcacgcac ctgattggca agctgtccca
    10681 ttgccggacg ttcctctgcg gacagctttc ccatctgctt caggataccg gtcagttcac
    10741 ctttttttcc cagatactgt acacgcaggg tttccagcac cttggaatct gcacaatccg
    10801 acaatgcctt tttggcagcc gcacggattg cttccaattg ttccttcata atatcgttcc
    10861 tttcttccgc aggttctgcg ggcatttggc tctatggcaa taccgtacaa agtcgccaga
    10921 cgctctttgt gcggtattgc tctatgggaa acacatatca aaaaagccct gtcctgtgaa
    10981 aacaggacaa ggctcacgct tgttcaaaac cacccatttt caaagagccg tcactcttct
    11041 gtccttaatg cgaacaccac atctgcacct actgtgaaag ccggttcagc acagccactc
    11101 ggaagggaac ttcagcatat gccgccggac gggttttcag cactccccgc tctctgtacc
    11161 ggcaggctct ccggcaacat ctttcagaaa atccgctgaa atttgccgcc gcaatatgcc
    11221 tactcgcttc gtctttgtgt tttcaaatca cattatacct gttttcaaaa aaaaaatcaa
    11281 gcccttttga aatttttttc ttgaattttt ttgaggagta tggtataatg gtaattggac
    11341 gcgacaaaaa gagaaatgtt ttcagcaact tgcacaaaaa tctgtatcac atcggtgttt
    11401 ttccagcagg ctgtgatcat ttnnnnnnnn nntaaaatcc gtccgctcag aatgcgaccg
    11461 gaacagacag ggcaatattt tgccggaacg ttcccataaa aggagttttt acagatatgg
    11521 ataattttgc agagtacatg gtaaaaaagc gaccggattc ccgtgacaat gccaagcgtg
    11581 ccggcatcat tgcactggca gtggtgcttt gtgccgcatc ggtctttctg gtgtttgtca
    11641 cacacgttcc gtttattctg ctgatcacct gtgcgatcat ctacggagca tatttcctga
    11701 tcacgggtac ttccgtagaa tatgaatacg ctgtcaccaa cggggaaatg gacgtggaca
    11761 agatcattgc acgccgcaag cgggtgcacc tcatcacggt caacactgcc aagttcgatg
    11821 cttacggcga aatgacagat gctgttccgg acgctcctga ccgcaccatt gtcctgtgtt
    11881 ccgacaatac cggtgagggc gaatattacg ccgatctgga aaccgaggag tatggtgcaa
    11941 cacgcatcat cttcaccccc aatgaagccg tgcaggaagc gatcaacgcg gctctccccc
    12001 gacagctgcg ttccggaaac ggagcagcca aatgagtgtg attttccgct atgtgcttct
    12061 ggtgctgttt gtgctggtga atctggtggc atttgccctg atgggcgtag acaagcagcg
    12121 tgcccgcacc caccagtggc gagtgccgga aaagacattg tttctggcag cactgctgtt
    12181 cggcggcacc gggggcacga tcggcatgtt cttctttcac cacaagacca agcactggta
    12241 tttccgcatt ttctttcccc tgctggcaat cgtccagctg ggcatctttg gttatctgat
    12301 gcaaatatta gggaaacact gaataaagct tgtgcgtaag atgcgtagtc agatttttcg
    12361 gcagattgca tagaaattcc gcaggcagac tgtatgtctg gcaagggatt tctgggcaat
    12421 atgacggaaa atctgcaagc agattgcgta caaagccgtc aggcgggctt tatgcagtgt
    12481 ttccttagat taggaggttt tctctatggc aaacaaattt ttccgtaaac tgaacgggct
    12541 tctgactgcg gcagccctgt gcgtatcttg tctgcctttc ggcggcatgc tctctgccag
    12601 tgcggcagac gtgcaggaaa caattccggg gatgacacaa tctccggaac agctgataca
    12661 ggaagctgac cgcggcgatt ttctgacgct gggctatgct gtgatgctgg aatgcagcta
    12721 cggcggtgtg ggatttgctt ccacgctctc cgaaaaaaac agtgtccttc tggacgtcga
    12781 ccattccggt gccatcgaca gtgcagacat tttcctgctg atgtactggt gtgcttactt
    12841 cggtgcctat ggcaaatata ctgacgaagc actggccttt ttgcaggagt gggacgctgc
    12901 gaacggcaac ggcaatacgg aaaccacaca gccgaccaca gttccggtgg caactgcaac
    12961 cacaacgctg accgaaacca ccacggccat tctcagtacg ttcccgccgg taccggaaac
    13021 cgctacggac agttccacag cttctgcatc tgctgaaagc aagaccacgc cggcagagtc
    13081 catggcggta acttcgcttc ctgcttccag cactaccacc aaagctacta cgaaagcaac
    13141 tacaacgctc ctcgccacca ccgctgctcc ggcaactacc acagtgacca ctacacagac
    13201 ggcaaccgga gcatgggcac agaaggattc ttaccgcggc attgacgttt ccaagtatca
    13261 gggcaacatc gactggaatg cggtaaaggc ggcaggcaat gacttcgcca ttatccgtgc
    13321 aggctacggc aaatacgcca gccagaagga tccttacttt gaacagaaca tgcggaatgc
    13381 caaggcggca ggcgttgcct gcggtgcata ctggtacagc tacgctgtca ctccggcaga
    13441 ggcaaagcag gaagcagaag tatttgccag cattgtgcag ggataccagt tcgaatatcc
    13501 gctggtactg gacattgaag attctaccca gaccaagctt tccaaggagc aggtatctgc
    13561 gatcattcag gcattctgtg acaccatgga atccaaggga tactacatca gcctgtacag
    13621 ctacgcaagt ttcctgaaca gctacgtcta tcagtcagtt ctggaggact acgacatctg
    13681 ggtcgcacac ttcggtgtca gccgcccgtc ttactccaag accagctacg gcatgtggca
    13741 gtattccagc actggttctg tcagcggcat cagcggcaat gtggatctgg actacagcta
    13801 taaatgctat ccgaacctga tgaccaagta tcatctgaac ggattttaag cgtacataac
    13861 agacaaaagg agcagcacca tgcagtgtgc tgctcttttt gtttttcgga gatttcagta
    13921 acgctgtctt tgtatttccg agaaaagtca aacgggcact ctggtatttt tccagagtgc
    13981 ccgcttttgt gatgtttgat tttcaagtcg ccttagggca acaccgcaca aagcttgtgc
    14041 gtaaggtgcg ttgtcagatt tttcgg
//
LOCUS       DAWBGR010000050        13911 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1389_length_13911_cov_3.871464, whole genome shotgun sequence.
ACCESSION   DAWBGR010000050 DAWBGR010000000
VERSION     DAWBGR010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13911)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13911)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13911
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1389_length_13911_cov_3.871464"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2867..2876
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3114 a         3603 c         3171 g         4013 t
ORIGIN      
        1 catcctccgc agatgtggca tcaccgcaca tcagcttgtg tctgccaact gtccatatat
       61 cgccacgctg gacaaaagcc gctttttcca gtgcagtggt gaggtcaaaa tcatcgtctt
      121 tgacttcatc accgctgttt gtatcaaata aatcagcaat ttcagattca tcgaaaccgg
      181 tcaaaccaag gtcaaatccg agattctgta actcttccat ctcaacagca agcaaatcat
      241 cgtcccagcc tgcgtccaat gccatacgat tgtcggcaag aatatatgcc tttttctgtg
      301 cctctgtgaa atggtcaaca tatacacaag gaacttctgt aattccttct tcctttgctg
      361 ccatgatgcg tccatgccca gcaagaacgt tgtattcctt gtcaatgatg acaggattca
      421 caaatccaaa ctcacggagg gaagaacgga gtttcagaat ctgttcttta ttgtgtgtac
      481 gagcgttatt tgcataaggc actaacttgt tgatgtcaac aagctgaaat tctgtggtca
      541 ttgtcatctg taattcctcc tctgctgaat tctgagcata cctcttcggg cggcatccat
      601 attgcctttg acagcctgtc cttttatcgt gcggtattgc tgtttggtca tgttgtttct
      661 atgctgtttc agttctctcc agaattgaac atctgctttc atgtatttct cactttctgc
      721 ttctcagtaa tttttccatc atatcttcct gcggattgcc ctgaaattcc acagagcagt
      781 tttcacggac tatctgaaaa atctgattcc agatttggtt tgcctgtttc atgtaatttt
      841 gtgacatcgc tacataggga gaggcaattg ccgcaccagt tgtaggatgt ttggaaatgt
      901 atccgtactt tgtgacaatc tgctcacagt gaatccaacg ggaaatgctc atggcatact
      961 gttccacaag ctgacggctg acgatcttct cgcaggaacg ttctttcagc cattgataag
     1021 tttctgtata cacatcatct gcaaggagtt ttgtgccgtc acgctgtaat tctttcatga
     1081 aatctctgac aggcggtgtt tcagcagatt ctatatccgc aggctgcatc ataacttctg
     1141 ccgattttcc ctcagcaatc ttctcagtga gtgcttttct cggcctgcct gcacccggtc
     1201 ttgcaccgcc tcggtttgta ccgtctttcg ccatgatgtc atcacctccg aaaaatcaaa
     1261 gaaaatcaaa caaaacttaa aattgggcat aaaaaatgcc gactgcaaaa gtcggcaaag
     1321 ttagctgtta tcagtgtttt tcagtatttt tatctctgag gggtcaatag ggtatttgaa
     1381 tacccgtttt tgtgcataag aggggacgcc ggtctgtaaa aaattcacaa ttagcgattt
     1441 ttatcccccc accggcagca tttcagacac aatcaatacc gatagacggg atttcggtct
     1501 tctgtccacg tcttgcggtc atggcaggac ttgcaaagag cctgccagtt gctttcattc
     1561 cacatcagat gcggatcacc acggtgagga atgatatggt cgaccacggt cgctgccgtg
     1621 aaccgtccct gtgctttgca ccgcacacac aaaggatgcc ggcggaggta tgccttgctg
     1681 acacgctgcc acttgctgcc gtagccacgc ttggcggcag atggtcggtc gggatgcagg
     1741 ggctgatgct ctgcacaata caaaccgtct gtcaagttgg gacagccggg atgcttacat
     1801 ggtttcagtg ccttcctcgg catagggaca ctcctcacag tcgaactcac cgccgcagtc
     1861 acagcccttt tccaccttga tgcgcttgag ggctttcttg tggagcttct tcacccattc
     1921 ggtggtgtcg cccagctcat cggcgattgc cgcccatgtt gcaccgtaga agtagcggag
     1981 gcggagcacc tcacgctcat cagcattggg attcgccatg atgatctgtt cgatgttacg
     2041 cttcatacga atggactcga taagcttcca acgggcatcg gtgaggattc cctgtacctc
     2101 ttcgtcatta aactcctcgg cgagcttctt ccactcgttg tagataacga cctgttcact
     2161 gatacgggaa cgaagccctg ttgcgttacg catgacactc tttgcaagca ttgtgtttac
     2221 ctccatttca tggcataaga aaagccgcac gatgcttttc ggcactgacg gcttcacttg
     2281 tatcttaaat ttccattata cagtatatca cattattttg tcgcattcaa gggacattca
     2341 gtcgcaatta gtcgcattca gtcgcatcct gtcgcaactt ttcatccaac gccgatacag
     2401 ccttatcatg aagccgatag atatgttttt tgctgtatcc catcttgccg gcaatcacct
     2461 cgaaggattc aaagaacaaa tatctgcgtt ctaacagttt tctgtagcga cggtcgtcca
     2521 gctttccgat agcatgttca atgtcaatct taatagcgat cattttatca atcaaagcat
     2581 cggcttcctg ttcaaaagcg ataaccttgc agatagcttt gctgattgca tcggagccac
     2641 cattaggaga agatccgcca ttgttttcat agctgacacc tctgccatac aaatcctgac
     2701 ggagtctttc agccttttcc atcttgtcgt taatcatttg attaagctcc catgcctgat
     2761 tcagatattc ctttgcgttc atcatgctgc ctccttaacc attctgcgga ttctcgccat
     2821 aagtacgacc gtatcaatat cgctgagact ctgcgcccac tcggagnnnn nnnnnncgaa
     2881 agaacttctc gttctccgca attgctacat catcatgttt tacgagtgca tccttgtaga
     2941 cggtcgctgc cgcttcgatg atggcaagac caagagcctt gtagggatcg tatgctgtag
     3001 attttctttc ttcaacctgt atcatttctt acctccaagt tctgccttga ccgcatcgat
     3061 cagtgcgttt tgggtttctt cctttttctg caatgccgac atgatttttc tgtcgatcgt
     3121 gccggttgtg atgatgtgct gtataatgac tgtcttggat tgctgtccct gtctccacag
     3181 acgagcgttg gtctgctgat acagttcgag cgaccatgtc agtccaaacc atatcagcgt
     3241 agaaccgcct gactgaagat tcagaccgtg accggcactt gccggatgta tcagaccgac
     3301 aggaagctca cctcgctgcc aacgggatat actgtcagcc ttatccattg ttgcgaacgg
     3361 aatatgcagc ttattgagcc tttcctgaat tctcgcaagg tcatgcttga accagtatgc
     3421 cacgaggacg ggctttccat tggcggcttc gatgatgtcc tcaagagcgt caagttttcg
     3481 gtcgtgtatc ggaagaatat ctcggtcatc ggaatatatc gcaccatttg ccatctggct
     3541 tagtttattg gatagacttc cggcattgct tgcggttaca tcaccgtcgg gaagctccaa
     3601 taccaactcc tccttcaatt cttcgtaatg ctgtctttct tcctcggaca gcttcacggt
     3661 atattcggag attataagtt caggcatatt caggtggtcg gtggatttca tgcttatcgt
     3721 gatgtcggat attttgtcat agatctgctg ctctgcatcg ggcagaggtc tgtatgaata
     3781 cacgatattt ccatttcgac ggtcgggcag gaagtaatct gtacgatatt tgccgatgaa
     3841 tcttccaagc cgctgtccca tgtcgagcaa tcggaactct gcccataagt ccatcagacc
     3901 gttgctggag ggtgtacccg tcagacctac cactcgcttt actttcggac gcattttcat
     3961 gagagacttg aatcgcttgg tgtcatgatt cttgaaactc gaaagctcgt caattattaa
     4021 catatcgtag ttgaaaacca cgccgttttc ttcgatgagc cactgcacat tctctcggtt
     4081 gatgatgtag atgtcggctt gagtttttag tgacctcagt cgctctgcct cagttcccac
     4141 cgctacgctg taggtcagat tctgaaggtg ctcccatttc tggatttcgg caggccatgt
     4201 atcccgtgcc actcgcagag gtgcgatcac gagtaccttg ctgatctcga agtagtcata
     4261 tatcaactcg ttcacggcgg taagtgttgt gaccgttttg cctaagccca tatcgaggag
     4321 cagagccgtt acgggatgtg ttatgatata ttcggctgca tacctttggt actcatgagg
     4381 tatgaatttt ttcacgggtc atcacttcca atcttatgaa ctaactctgc aatatctttt
     4441 ttatcatcca gcacataaac ctgaaagccc aatgctctaa gctgtttatg cctttttagc
     4501 tgaatttttc tcggaacatc tccgggagct tttgtctcaa caaaggcgat cctccctttc
     4561 ggcatcaaga caattcggtc gggaacaccg gatgtgccgg gagaagtgaa cttccaacag
     4621 agacctccga ccgccttgac tgcatcacga agtttcaact cgatgtccct ctcacgcata
     4681 ggctctctcc tcgtacattt tccacaccgc tttgaacaca tcaatgcatt cctgcgatgc
     4741 ctcacgctga tacaccagat agtcgtaaac ggcatcatag ttatcatctt ccgggaagaa
     4801 gtcatccctt gccatgtcat aagccagatc accggcaggg tcatcatgcc caagaaagca
     4861 tttcactacc cagcggtgaa aggcacttct cttcggtttc tcacgttcca tatatctcat
     4921 catgttaaaa actcctattc tacgaggttt ttagcttttg gtgacagtct acgacagtca
     4981 tttctatacc cttatatata gatatttttt tacatttttc tctcgcctgc gtaaagtaag
     5041 gatatgagtg tcctcgactg tcaccttggc tatttttcta cctttttttg acttcaaggt
     5101 gacagtcgtc agttcaggaa atcgtccaaa actaacctca atcccataat cagtcttgcg
     5161 gtcttcgttt ttttcttaga gaatcctgcc tgctccaatg cggtataaaa atcagttgta
     5221 cttctgacaa attccccatt ctgcgcacaa tagttacggt actcttggta gagatcaccg
     5281 gacttttcag tgtaggactt atcgacctca cagcattcag caataaaatt tccaagccag
     5341 tcgttgtcat cacggtacga gccgatcgcc gccttaacgc aatcgggagc tttgatctgg
     5401 taatttgccg aaacaacctt ttttgcacct tcgatcagcc acttcaaaac tgcacctcca
     5461 gcgttgtcaa tgaggtactg cgtgtaattc ttgatatccg ctgctccctg aatctttgcg
     5521 tggaaaggaa tcacgatcag tcgtctccaa gtaccgtcat cagaggctcc cacctttggc
     5581 aggtgattcg tatagaggat cagcgtgtgg gaaggctcga aatcgaacgg ggctttgaac
     5641 tttttctcgg caaaaatgct gtcagtggaa cagagctgtt ttaccacgga ggtgttcagt
     5701 ctcatgcctt cctgtaactc ggcagcgata atcagtcgtt tacccttgag ctctgccatt
     5761 tcgggcttga cattgcgctt gcagttcatg gtcaaagcat cagccgacat attgccgctg
     5821 tagcttccga ggactttgta gatgacattc caaaaagtcg atttgccgtt gcgtccgtca
     5881 ccataagcga taataagcga ttcaacgtat acttttccca cgatacacag accgcaaatc
     5941 atctgcacat agtcgataag ctgtcggtca ccgcagaaga ataattcaag tgcatcgagc
     6001 cacaactgca taccctcgtc cgagggaaca acagcggtca ctttggtcag caggtcttca
     6061 gggttggtag ctctccaacc gtccatgcca tcggggaggt agtaagtgcc gccgggcgtg
     6121 ttcaggagca ttgcttcttt gtccagtgct tccggcggct tctgcaccag agcttttgcc
     6181 gcatcaaggg cgttgttcag accacgcata ttgcggtatt tcatgacgaa tgccttaaac
     6241 gactttgcaa agctgtatgc ctcataagca tcaagctgtc catcgaagag agactcaacg
     6301 aatttctttc cgcccttgag agcctgttcc atgttgatgc ctgcgttctt cattcgttcc
     6361 agagctgtct tttcaagctc atcagctttg ttgagctgtg catcggtgtg ctccatcatc
     6421 gcaagcaccg ccgactgctc gttttcttcc caataagtgc cgttgtatct gagatagtca
     6481 gtcgccttgg tgaaaacaag ctcatcaccg aaacagtccg caagggtacg agcttcaccg
     6541 acatcggaga agtcatcggg gatcatgctg ttcttgccat attcttcggg gctgacatag
     6601 ccttcctgct ttgcgatctt tttgccgaac ttacaggcag aatgccatat cgtttcgagt
     6661 tcctcgtcat caagcggagg gtcacactcg gcagccattt cgagaaactt ctcgtgtgca
     6721 tcttcgccga taccgaaccg cttcacgata cgacctgcct ttcgggacat ggtcgcatta
     6781 cgctgtccct gcggaatact gcggttggac ttcatgaaaa gaagccagtc ctcgatggag
     6841 agcgatccct catgccactc gacttctccc gtactgccga agaagaagcg agctgcgtca
     6901 agagcgttgc tgtcgaagaa cggcatttct ttctgaatgc ggcgtttcat cttcttgtgc
     6961 atggtggcat ccttgcaaat gccagcaggg agatagacgt gcatcttggg gcgagcggtc
     7021 ttgccgtttt tcggtatcat gtgactgcgg ctgtatacga tagcgtatgc aacatcggtg
     7081 aggatcgccg acagactctc ggcatcgatc cattcttcgg ggtcatcgga atgggtgttg
     7141 tcgcagtcga gtacaagtac atcggacatc tcgaaattcg cctcactacg gacatttccg
     7201 gcatatctgg cacatacatg gtcgaagcgt acagctttct ttaggtcagc cgccgaggtg
     7261 acgatcatct cgtggggata agccttgttc ttcggattac cactgcaatc agcggtgtag
     7321 agcgtaaatt tcattcgttt tcctccttct caaaaatacc gttgcatttc gggcagtgca
     7381 tgaagttctt ttccttgccg aggtctgcat ttccgcacac agggcaagcg gaacgactgt
     7441 ggtgatgttc ttccttacag ggcttgcggc attccttttt gcgaagctcg gcaatgacag
     7501 cgtcaagcac cttgatgtcg tggtagaata catcattgaa gtcgtcatcg agtccgatat
     7561 gcgactccgc atctttgcgg agcgacatca gccctgcaat aatttcgctg atcctcattt
     7621 ttttcctcca tttcttccgt aaagtagcgg atcttcatgt atttgcgttt tgcctgtttg
     7681 atctcctcgc tcatgccctt ggagatcaca tcgccgaaca cccacagttc acagcagtga
     7741 ctgaggagaa tattgttcat cagcaccgcc tcagctctct cgtcctcatc gttcataaac
     7801 tgcgtaaagt agatgtgagg tgtgataggc aggcaatggt tgtccaccgc aaaacggctg
     7861 tatttttttg cgttctcggt gttctgctcg atgttccctg cgtatggcga gcagatgtat
     7921 acgataggac gaaagtttgc ggcttttcgg gcagcctttt cttccttttc gatgcgagtc
     7981 atcgcctcat attcggtagg gctgaagtag ccctcggagt tgtagagatt tgccacgatt
     8041 tatacctcct ttgccagcat ctcacggaga atagcggtga agatctcacg gagctttttg
     8101 tcgtcgttga tgatgtcgag gacagacacc gccttgatag ctgtcttggt tgcaccattc
     8161 tccagcagtc tatttttctt attgcgcacc ctcgctgtca ggcatacacc tgcacggtac
     8221 tccagtgcgt cataggctct ctgatagacg gactcaatgc cggagtagtc gccgcattct
     8281 gtcatagcaa tcttgccgac catactgcgg acatcatcac gccagtcatc gaactcgacc
     8341 gtaaatgctt cctgaatggc attgaccttg tcaccgaccg ctgtaatttc agcctgctgt
     8401 gcattgagct tctgttctgc ctcgatcatc cagcggagtg tcggggaaag gctcatatcg
     8461 gcaaggtaac cgccggtctt gcggatcgca ggcagtacct ccgaggtcac ccaatgtttg
     8521 aagcgttttg ctgtggggag cttgctgcaa agaacgaggg agtagagacc gctttcgttg
     8581 ataacggtca gcttctgtgt tccgccaagg gtgtcgcatt ttgcgacatc cttatcctca
     8641 tcgtcaacgt gcttgaaaat tgcatctctg gcattgctgt atccgagagc ctttgctaca
     8701 tctgctccta cgaaccacgg ttcaccgtta atgtccattg ttttgatttc accgaattcg
     8761 gcatttttgt aaatttcgta tttcatgtag atattctcca attctatctg tccgtattgg
     8821 tgcggacagt ccttttcgtt agttatactg tctgtattgg ttgtttgtgc gtctttacca
     8881 gagctattta acgtctttta aagcgaagtg cagctcacac cactttatcg ctgtaagtgt
     8941 atgctcacgg ctgtctgtaa accagccatt cgtcaccata atcgtgctga tctttcttga
     9001 aatgcagtaa agattctcta tgctgaaatc tgtcttgtcc gaattcaaaa agacgatcac
     9061 atgacctttc ggcactttct tgccgtggtg ctgttcccaa ataagccgct gtttttcgat
     9121 ataatcctgt gtcttaattc ctgtgtcttt gattttcaca taggtgtatc cgttggaatg
     9181 gatcgactca ctgcctacag gagcattatt ccatgtctga tgtccctttt tatatgcctg
     9241 tctaccggat attagtttga gaaatttagc acaatgtgcc tgaacacctc tttcagtctt
     9301 ttccgcacca aatcgcttat tgaaagcatc ggctactgca acataattag cgtactcatg
     9361 gcaatgctct ctgagccatt catcctgctc ctcggtaaaa ggatgatgaa cacccgctat
     9421 accaaggctt ctgcatttat ttttcagcga tgatgctgta acgaagagtc caaaataatc
     9481 tgcaaactgt atcgcaagtt ctgcataagt tgcagttccg actttatcac gaagccactg
     9541 catttcctgc tccgtatagt ggtgcatctg cctcatcgga tcagcttctc gatattggag
     9601 ttctccagct ttccttcagc gaccagcttt tcggtgcgga gaacgacgtc agcgttgttg
     9661 atcatctgct tggcgagcga ggagatcgtc tgagaaagct cagcctcctt cttgcgctca
     9721 tccatactca tttcagtatt gcttacgatg cggattctct caccgagaat tttctgtaat
     9781 tccataagtg tcataaaact tcctcctcaa tctttcatat aaaattcgca ttcatatccg
     9841 tctgcacgga gcgtcagtcc tcttgcccat cggggagtga tacacatttt ctgcacgatc
     9901 tcatcaacgg ttatatcgtc agtagcttcg ataatgactt catcgtgtac atgagcgaca
     9961 atgcgataat ccctgagatt catcattgca tggagcagaa tatctcgtgc aatgccctga
    10021 atgatgttct ccacgaactt cggtccgtag ctttcgatac gttcccactt tttctgctca
    10081 ccaacaccca tgtaagtgac cgattcgccg ccgaacttgt tttcgccgat tttcggtttc
    10141 acataggcaa gtcgtctgcc ggatggaagc tcgatgaaca gaaatcctga ctcacagaag
    10201 atatgcagtc ccttgacatt agcctctgtc ttttccttga tgcacttttt tacggcacgg
    10261 tcaacatccc accagagctt tacgatattt ggagatgcat ccctccagtc cgtcacgatc
    10321 tgctggatct catcatcaga cagtcccatt tcctgtgcac ccattgcttt catcgcacct
    10381 acggagccgc catagccaca ggcgagctcg gctattttac ccttctgtcg cagatgccca
    10441 ttaataccgt gtttcacaac gggaactccg aatatctttg atgcagagat acagtagatg
    10501 tcgccgccat ttttgaacac atccaggcgc cactgttcac ctgcaagcca cgacagcaca
    10561 acaccctcaa tgctcgtgaa gtccgctaca atgaagcgtt tgtatggggc tttcggtaca
    10621 agagccgtgc ggataagctg tgacagtaca tcgggaatat ctccgaagag gaggtcgagc
    10681 gtttcgatat catcggcttt gatgaggtct cttgctgtat cgaggtgagg catcgaatta
    10741 cgataaaggt tctggagctg taccagcctc ccgctgaatc tccctgaacg attcgcaccg
    10801 taaaactgaa acattccacg aatacggtca tcgatgcaat gagaactgag catcgcatcg
    10861 tatttcttaa cagacgactt cgcaagctgc tgtcttaggg agagtacctt tgcaagctcc
    10921 ggcggcgctg tctccaatag ttcctttact gcctgttttc caagggactc cgtttccatg
    10981 cagttttcgg caagccactc cttcatctgc tgtacggaat ttgggttgtc aagcatggta
    11041 agtgcctgaa tttcggcagt gatctcgttt ttgatgtcct cattgatcgt gatagcgtga
    11101 cgcacaagct ccatatctac accgatacca cgatcattga tctgctggtc gaggtgatat
    11161 tcattccata caaaattcgg tacgggatat ttggcgagcc tttgctgaat ggcgatctcc
    11221 acctcaacat cccggcagtt gtaggacttg aacagttccc atttgcccgg atgtaccttg
    11281 ggatgatgac ggttgccgtc cttgtcagga atacagaaga acttgatgag gtctttgccc
    11341 tcggacatct tctgtttgtc cagaccgaga actgcaccaa caccggcaag ggacatgggc
    11401 agaccgagcg ttgccgccca gaccatcgtg cagtgccact gtgtcgggtc aagatatgtt
    11461 cccttgggaa gtccgagatg cttggaaata cagatgcgct cgaacatggc attgtaggcg
    11521 gtcttgatga cattcgggtc agtcagtgct gctataatat cgtcggggag ctgttcaccg
    11581 tttgcaaggt caatgacctg cggcggatgt ccgtccacgc tgtagccgaa gaacagaatc
    11641 tcaaaattat cggattccgc atagggataa actcccgatt tactcagatc aacatccgaa
    11701 taggtctcta tatcaattga aagtattctc atagttcaaa taatcccacc caagccgaag
    11761 cccagcgccc cacccgtctg tattcttagt tcaggaaatc gtcatcgtcc tcatcatcga
    11821 ggtcagcaaa gtcatcttcc gcactggaac ggctaccgag aggctcaccg tcacggagct
    11881 tctggagatt accgagaccg caggcgatac cacgggcagt tccggtattg tatgcgtaga
    11941 aattgataga tgctctgccg taaacgccgg agtacacctc gctgtgagta agaatcggct
    12001 gacgatcagc atccacgatc tcaggtgcag tgatgctgtt ggcattgacg aagtatgcgt
    12061 ttgcgtaggc ttcatcatct ggacgctcga tgtcaccgtc acgaagaggt gtcttgatcg
    12121 ctgccagagc cggtacggac ttgccgttgc ccttgagctt gcctgcacct tcctcgtagg
    12181 cagcctgaat tgctgccttg atcttctgaa tggtgacggt gtcgctcttg gggatgatca
    12241 gggaaacgga atacttctcc ttgccaccgt cctgacctgc tttcggttcc catacatttg
    12301 cgtaagacca gcgggtattc acaccggtca cgaccttggt cggatttgtc atcttctttg
    12361 ccatgttact gttcctcctt gaaatcttca tttgcatcgg aatacacggg acgcttgtcc
    12421 gtttcgggaa cgagcgtcgg tgcgcccttt ggtttttcta catatccgct gagaatttca
    12481 gcgaatttct tcttgccgag cagcttttcc attgcagtga tgcccatgat ctcaggcgca
    12541 ccgtaggggt catagcctgc atcggtgaca gcttttgcgg ctgcatcctt gtcgatgtat
    12601 ttgcggacgc tgcgaccttc gaccagctta tagccgtcat aatgaactcc gctgagagcc
    12661 tgtgcaagag catactcctt gacatcattt gcccatgtga ccagctcact cgccttggag
    12721 agaatgattg gaatttcatg ctcctcaagg ttgtcgggag gagcgaactc atactgtgca
    12781 aggagcaaat tctgttcagc tcttttgcgg cagacagcct ttgccttaca gaaacggcag
    12841 tgacttccgg cacagaattc tccctcaccg ttataggcaa gctgtgcggt cggtttcagc
    12901 gtgtgttccg cccattcgag cagctctgcc acgctgatct cccactcatc tacattttca
    12961 agtctcggct gatagatgat aaggcgaacg gtgttgatgt catacaggaa atcaaacatc
    13021 gcaagtgtgc ccaatgcgta ggtcttgagc tgcggattgt cggttgcttc gacctttata
    13081 cccatcccgt gcttgtaatc cgatatggtc atcgtgccgt cagcaacaat gatgcagtcg
    13141 gcggtaccga agcattccgg gatatattcg gacacatcaa ctcgctgttc gagcatgatc
    13201 tcggtcgtgg tgcaattctg ctttgcggtc tcatacagct ccatgatgta agccgcatag
    13261 ccctcggcgc actcctgcat ttcagcgttg tagaacttca gctttttctt cgggttacgg
    13321 actttctcgc ccagtgcctt gcgtaccaga tattcgcaaa gctcgtgagc ctctgttccc
    13381 tccgcagcaa agtcgctttc ggtatcgggg aactgctccg agaatcttgc ggacggcgga
    13441 caggcaagca ctctggcgat ggacgaaggt cccacccacg cgtgtgcttt cggcggcatt
    13501 acatcatcgc ctccaattct gcgagcattg cgggatagac ctccgctttc aggtcggaga
    13561 atttctgacc gccgtacttc tggatgagtt cccttgcttt cgccttatgt ccggctctgc
    13621 tgaactcgga catcttggcg aagagcacct cgctgacatc gggctgcgga ggttctgctt
    13681 ccttaatttc aggctgatct gtttcgggat cgtagatcgg aacgaagtcg tcggggttga
    13741 taggctcggc gggtttctca caggcgatat gctcattggt tgtcacagcg ttggcatatt
    13801 ccgcaaggct gtctgcaaga tgtctcatgt cgctgacaac atccagcaaa agtttgattt
    13861 ggctcatgtt tttcactcct gttacaatat aattagtcgg gtaacacctt a
//
LOCUS       DAWBGR010000051        13860 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1392_length_13860_cov_6.403912, whole genome shotgun sequence.
ACCESSION   DAWBGR010000051 DAWBGR010000000
VERSION     DAWBGR010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13860)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13860)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13860
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1392_length_13860_cov_6.403912"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    12457..12466
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3202 a         3441 c         3750 g         3457 t
ORIGIN      
        1 caagcagatg gcgtgcaaag tcgccagacg ctctttgtgc ggtattgcct tatatttcaa
       61 tgtgcttttc ccagtgcact tctcacccca gcaacgaccg gtacagttcc acatacttct
      121 ccgccgaaac attccagctg tagtcgcact tcatggcgtg gcgtaccaga gcgttccact
      181 tccgcttgtc tgcgaacagg acgcaggcac gctttactgc gtccagcatg tcgaatgcat
      241 cgtaggtctt gaaggtgaag ccgttgccgt cctttccgcc ggcatcaaaa acagagtctt
      301 tcagaccgcc ggtttcccgt actaccggaa ttgtgccata ccgcatggca accatttgtg
      361 ccagtccgca gggttcgttc tgggagggca tgaggaacat atctgcaccg ccgtagatcc
      421 gccgtgccat tgccggcaca aagccaacag tcacatgcac ctgttccgga tattctgcct
      481 gcatttcgcg gaagaagtcc tcgtacagcc gttcgccgct gcccagcact acaaaccggc
      541 agcccagttg taacatttcc tgaaatgtct gctgtaccag ctgaatgccc ttgtgtgcta
      601 caaaacgagt cacaatgccg atgaccggag catcagaatc agtcagcccg cactctttca
      661 gcagggctgc acggcattct tttttgccag ccattttggt gacagaataa ttctttttca
      721 ggttcgggtc tgtttccgga tcgtagccga taacatcaat gccgttgaga atgccgacaa
      781 tgccgtcctg ccgccgcacc agtgcacgat ccatgccgta ggaataccac gggtccagca
      841 attctttggc gtaggtgggg ctgacggtag tgatacggtc tgctgtttca atggcacctt
      901 tcatcagatt caccatgccg tcaaattcca gcaggctggt gtgataggtg ggaattccca
      961 ttaactctgt gatgacttcc ttgccgtaaa cgccctgata ctgaatgttg tgaatggtgt
     1021 aaatgatacg gatattttcg aattcctgat gatactggta gagcacacgg tagaacaccg
     1081 gaaccattgc ggtctgccag tcgttgcagt gaatgatgtc cggcttccag tccagagccg
     1141 gcaggatctc cagcacggca cggctgaaaa agacgaagcg ttcgcagtcg tcatagaagc
     1201 cgtacatgcc gtcccgcatg aagtagtagg ggttgtccag cagatagtag gtgaccttgc
     1261 ccacctgaat ggtatagatg ccgcagtact gtttccgcca gccgacctca acggtgatgt
     1321 ccttgagata tgtcatcttg ttgcggacgt tgggagcgac actgcggtac agcggcatca
     1381 caacccgaca gtcgatgtcg tcatgtttgc ccagggcagc cggcagagaa ccggcaacat
     1441 cggcaagccc gccggaagcg gcaaagggca gggcttcact ggctgcaaat aaaattttca
     1501 tggtatactc tcctccaagg cggcagcaca caggctgtgc tgcaaaggaa acactgcata
     1561 aagctttgca cgcaatctgc cgaaaaatct gacgatgcct cttacgcaca agctttattc
     1621 agcgtttctc aaaaaactgc cgtatgcacc gtttcacagg caaacagcct gtgtacgccg
     1681 gacgcatacg gcagaaattt ttgttttcac tccattgata aaaaggctct tatgaaaggc
     1741 aataccgcac aaagattgtg cgacagatgc gtcgtcaaat ttttcgtcag atgaaacaaa
     1801 aagcggagtg aatactggat gtattcacga gcatttttgt gaaatatgac gggaaatttg
     1861 caagcagatg gcgtgcaaag tcgccagacg ctctttgtgc ggtattgcct aagaaaagct
     1921 tttagatcgt tgcctttttg cccagataca gcggatattc cggagaaccg gtcagcacgc
     1981 ggttttcaga aatggtcacg tttttgtctg caacaatggc atcaatattg cagccctcgc
     2041 cgatctctgt accctgcatc agaacgcagt tgcgtacaac agcacccttg gcgatcttga
     2101 caccgcggaa cagaacgcag ttttccactg tgccctcgat catgcagccg tctgccacca
     2161 gagaattctt gacgttggat tccagaccgt attttaccgg tgcattgtcg ccgaccttgg
     2221 tgtagatcgg agcttcgctg ttaaagacgt tgttgcgggt gtccgtatcc agcagagcca
     2281 gatttgcctt atagtaagct tccagactgt cgatcttgaa gaagcgtccc ttgtgttcgt
     2341 atgccttgaa aacgaagtcg tccttccgtg cctgaagcag gtcacggatc aggcttttct
     2401 ggttcttgct gatcgcttcc agaaccacct gctttaagaa gtccgtggtc atgatgaaca
     2461 tatccagaga gatgttgcac tcgccggaca gcatcggatt ggtcagaact tccttaatgg
     2521 tgttttcttc gtcgatttgc agaatgcagc cgtttgtgct cttgtcgcga ttgtacagac
     2581 ccttggcata gatcacggta atatctgcac cgctgctctg gtgctgtgcc agtgcatcag
     2641 tgaagtcgat cgtggttacc agatcgctgt ttgccagaac gacatactct gccttggtgt
     2701 gttctacgaa actccagata ttgctcagtg cttccagacg accctgatag gtaccgccgc
     2761 cgacctgact gaacggcggc agcaggtgca gaccgccctt tttccgagcc agatcccatt
     2821 cacggccgga acccagatga tccagcaggg agccgtagtt ggacttggtg ataacgccga
     2881 cttcggagat gccggagttt accatattgg aaagcggaaa gtctaccatg cggtaacgtc
     2941 cgccgtaagg gatcgaaccc attgtccgca gcttggtcag atcgatcact gcggagtcat
     3001 tgatacttgc aaaaattaac cctaaaacat tctgattgat actcatttta cgccaccgcc
     3061 tttacagatt ttcggaaata atttctttcg gaagaacacg cttgccggaa gagatcgtga
     3121 tattatgacc gagaacggcg atgccccaat cgtcacggtt cgggacggat tccggattca
     3181 taccgatcat ggcgtcagat tcaaccacgc agttttcacc gatgatcgca tattctacaa
     3241 cggcgttttt cttgatggta gtgccgggca taacaatact atacttgaca gaagcacctt
     3301 cttcaatggt gacaccggag gagatgatag agaagtccac ggtgccatct accacgctgc
     3361 cctctgtgat catggagttt tccacgtgtg catttttacc gatgtactgc ggcggcatgt
     3421 tgttgtggcg ggaatatact ttccagttcg gatcgtacag gttcagcggt acgctggggc
     3481 tgagaagatc catgttggct tcccacaagc tgtccagtgt gccgacatct ttccagtagc
     3541 cctcgaattc ataggcgaac aggcgttcgt catttgccag cattgccgga ataatatcct
     3601 taccgaagtc cttggaaccg gtgttggcgt tttcattttc gatcagatac ttccgcagct
     3661 tctcccagga gaaaacatag atacccatag aagccagtgt ggatttcggt tccttcggct
     3721 tttcggtgaa gtctacgata cggccttctt cgtcgcaggt catgatgccg aagcgggaag
     3781 cttccgaacg gggaacgtcg ataacagcaa tggtgctgtc tgcattattg gcgatgtggt
     3841 agtccagcat tttggagtaa tccattttat agatatggtc gccgcccaga acgatcacat
     3901 attccggatc atagcgttcg ataaatgcca tgttctgata aatggcgttt gcagtgccct
     3961 cataccaaga tgcccctgcg gcggtctgat acggcggcag gacatgaaca ccgccgtgca
     4021 ttttgtctaa atcccacggc tgaccgtttc cgatgtactc gttcagcacc atgggctggt
     4081 actgtgtcag aacgccaact gtatcgatgc cggagttgat gcagttggaa agcgggaagt
     4141 cgatgaagcg attcttaccg ccgtagggga ttgccggttt tgcaactttt tgagtcaggg
     4201 catacagccg gctgccctgt ccgccagcca gcagcatggc gacgcacttt ttcttgatct
     4261 tcatgatatt cctcctaata taaattcaaa attccgtatg gaatgggtca agctaaactt
     4321 ttgaatacac aaatatactt taggcaataa gggattacac actttctgac tttgtgactt
     4381 tcttttttcg ggcggaaacc gatgctgtcg gttttgctgc actttctgtg cggatcacgg
     4441 tttttttcgg ttttacggct gcttttttcg ttgtggtact tttcgccgca gtggtttttc
     4501 gcggtgcccg ttttttcact ggtgcatatt ccagatacag tacagacagg ggagccagtg
     4561 tcatagaaat gcactgctca aagccgtgca tggaaatcgg cagggtttcg aatttctttt
     4621 cggtgatgcc gctgccgccg tagactgccg catccgtgtt gaatacagtt tgatacctgc
     4681 cctctttggg aacgccgatt ttgtaatcgt tccgctgtac cggacagaag ttgcagatcg
     4741 caatgatctc ttttccgtcg tcgtcgatgc ggcggaatgc aatgacgctt tgctggtagt
     4801 cgtcgtggga gatccagctg aatccttgcc acgagtcgtc gtcctgccac agtgcagggt
     4861 gttccagata gaacttgttc agatcctgtg aaaactgttc catttgtttg tgagccggct
     4921 gttccagcag ctcccaatcc agttgcgttt cataattcca ctcgtttttc tgagcgaatt
     4981 cctgtcccat aaagagcagc ttcttgccgg ggtgagccat catatagccc aggaacgcac
     5041 ggtaagaggc aaatttttct tcctcgttct gcccgctcat tttctggagc atggagcatt
     5101 ttccatagac gatctcgtca tgggagatcg ggagaatgta gttttccgag aaacagtaga
     5161 agaacgagaa tgtcagcttg tcgtggttga acgcccgata gatcgggtct aatgagatat
     5221 agctgagcat atcgttcatc cagcccatat tccacttgaa gttgaatccc agaccgccca
     5281 tatccgtggg cttcgtcacc atcggccatg ccgtggattc ttctgcgatc atcagaatgt
     5341 ccggattccg tgcaaaggcg gcttcgttca ggtgccgcaa aaagtctacc gcttccagat
     5401 tttcgtgtcc gccgtagacg ttgggccgcc attcgccgtc cggtcggtcg tagtccagat
     5461 agagcatcga tgctaccgca tctacccgca gaccgtccag atgaaaatct tccagccagc
     5521 agcaggcact ggagatcagg aacgagcaga cttcgccttt tccgtagtca aatactctgg
     5581 tgccccagcc cttgtgttcc attttcaggg ggtcggcata ttcatagcag tatgtaccgt
     5641 cgaactcgca gagccccata ggatccttcg ggaagtgtgc cggcacccag tccagaatga
     5701 caccgatgcc tgcctgatga aacatgtcta tcattttccg gaagtcgtcc ggtgtgccgt
     5761 gtcgggaggt cggtgcgaaa tagccggtga cctgataccc ccacgagccg tcgaacgggt
     5821 attccgtcag cggcataaat tcaatgtggg tgtaggacat ctttttcaga tagggaatga
     5881 tctcctctgc gaagccgata tagctgggga acttttcctc cggcttgtgc ttccacgaag
     5941 aaatgttcac ctcgtagatg ttcatggggc ttttgtaggg aacgtgtttt ttcttttcca
     6001 gctgccagtc gcggtcctcc cagacatagc tgctttcgaa cagcttggaa gccgttcccg
     6061 gccgtcgctc atagtgagcg gcataggggt cggctttgtc cagaatctga ccgtcttcag
     6121 tttcgatgct gtatttataa ctgtcaaatt gtttcagtcg gggcagctct gcctcccata
     6181 ccgtgtcaga gatcttgtac atcgggtgac aggtgcggtc ccattggttg aatgaaccga
     6241 tgacagatac actcttggcg tgaggtgccc agacgcggaa acgatagaac tgtcctctgc
     6301 ctcgtcggaa cgggtgtgca ccaaagaatt tatgtgcttc aaaatttttc ccctgatgga
     6361 ataaatacag tggaaaatca tttgcagtgg ttttctgtgc ggattgcata gcataccctc
     6421 cttttgtttt ctattcatat tttaacatat ataggaatgt attgcaagtt atttttgtgg
     6481 taactagtaa atttcataga aacaaccaga attaaacttg tattttcggc atgttgcaca
     6541 agcagataga ctgagaatag acactatgta tcacatttgt tacgactgta gcagtcttgc
     6601 ggcacaaact gtcacgtcgt cccgtcgatt tccgccggca aaaatttctg ccttgtcaca
     6661 aatttccatt acgatttgct ccagatcgct gctgctctcc agaagctgcc gaatgagggg
     6721 atacgtttcc tcgctgactc cgtcggacag catcagaatg atgtcatcgg ggtagagcat
     6781 cacgtgccgc acagaggctt cgccctccgg ctccagtccc agcgggaatg tggtggcact
     6841 gatgcgggaa acctttccgc cgtggcggat cagggtggcg gcggcaccgg atttcatcat
     6901 ggtcatttcg ccggcatcga gatcaaagcg ggcaacatcg agggtggcaa aggtttcctg
     6961 cggggacttg gtgagaagca gtccgttcat catgcggatt gccgccgtcc cgcaaatgcc
     7021 gccgctgata aacttgcgga acagttctgt tgtcatgcgg gattcgatcg cagcattttt
     7081 gccggtgccc atgccgtcag acaacaccag atagggattg ccggcgttgt cggtgaacag
     7141 cagtgccgtg tcgccggaga tcgtttcctg actggcgtgg agggcggaag tgaaatgttc
     7201 cagccggtac ttcggtcgct ggcagagccg atagcgaacc gtgtcaccgg agaagatcgg
     7261 atccagttct tccagtgtca gattcagcga ttccgacaga atgtgtttga tcgtaggcag
     7321 gcagccgtcc agctcccgtc cctgacagca catttcgatc atcagccggt cggattgtgt
     7381 gtggtatacg gcggcggagt cacaggggta gccgtagtgc agcagcttcc ggcggacagt
     7441 gtcgctcagt tcgctgctgt agcggatatg cagatggtca ctggtcgcat gcagcaggtc
     7501 ttctgcggct gccagctgtt ccagaagtac cgtgcggctt tctgctgtcc gtgccgccag
     7561 aaaccgtgcc ttccggcggt tggcggcgtg gcgggaaaac agtgcccgca gtcgttcctt
     7621 ccggctgcaa tgtgccagtt cctccggaat cggcggcagg tctgctccga ggtgggattc
     7681 cattttccgg aagccggtga gggtttcctc gtatgccgat tcccagcacc gcagcttgtg
     7741 gcggcagctg ccgcagacgg tttcgcagac ttcacgggtg gcatctccgg tgggttcttc
     7801 ctgcggcaga atggctgcaa tggcatcggt atcttcccgc acactgcgaa tggcatctgc
     7861 catgtattgc agacgggagg acaggggcag cgtgttgtcg gaacggtcgg gcaggtcggt
     7921 gaccagccac ttgtccagcc agctgctgtc cagaaacaga aaggcagcac tgcccagaaa
     7981 caggttgatg acagcggaga tctggagcag gctggtgccg aatgtaatca gtgccataag
     8041 cgagaacagg aagaatacgc cggcgatcag gaaacggttt ttttctgcca gataccctac
     8101 cagcagaccg cccaccggca ggatcagcag gggcatgccg gcttccggcg agccgaggat
     8161 cgcaccgcag gcagagagtg caccgcagat cacaccgccg gcacagcgaa atttctttgc
     8221 cccgatcagc gtaactgcga cgctgatgat gccgccggcg ttcatggagg gaattccata
     8281 gcagctgaat gctgccaccg tcagaatcag aaccactgcc gcggcacagc cgtctgtgga
     8341 acgcagcggg atctttccgg tagaacggac gctggcaaag acagcgtgca taaagtagga
     8401 ggcacagccg gtgagggcgg cagtcatggt gtagccgatc acctccgcac cgctggcgtg
     8461 gaacagcagg gaaacgacaa tgccggagaa aaagacacac agtccggtgc tgcatgcgat
     8521 gccggcggaa gttttggcag ggcgtttcat cacccgcagg cagacgacca ccaccaatgc
     8581 aaacagcagg ggcagattgt ccagcatggt gttggagatg aggtaggaga tcaggctgcc
     8641 ggcaagcacg gaaacggcac ccgcagagcc gaggttggcg gcaatggcaa tgggcagcgg
     8701 agaagtcatg ttgcccacat aagttccggc gaggagcaga ccggagagaa atccgccgca
     8761 gctctcatag agcatgcggg gaacgcgggg acggcggaga agctgggaac ggctgggatt
     8821 gtgcatggtg gaaacgtcct ttctttacgg cggcacggtg atgctgcctt gaaaatgatt
     8881 tgtgcggtat tgccttgtgt atctattata acgatatgtg gaaaaaatat ctgtcagaat
     8941 ctcttgcacc attttaggac aatgtgcttg aatctgcggc ggcagggcgg gcaatcggga
     9001 gaaggtgcgt ttccggtcag tttggcgaaa aaatcagagc gtgttcccgt atttggagaa
     9061 cacgctctaa ttcctacttc ttctgatatt ccgccagcac atgctgcaat tcctgaatgt
     9121 acagttttgc ggcacggctg gtctgtgtct tggggttgga gatccagccg atctccatgc
     9181 gttccgccgt atccagcggc acagaaacaa tgtcgccgcc gttcaggtcg gcactgacca
     9241 taccgctgca aatggtgtag ccgttgactc cgcggagcag gttgaacagt gtcgcacggt
     9301 cgctgacgcg aatactcttt ttgtgcggca ctgtgctgag cagttcctct gcaaagtaaa
     9361 aggagttgtg gacaccctgt tcaaagcaca ggaacggata tggctccaga tctgccgtgg
     9421 tgacggaact gcgttttgcc agcgggtgag ccgcccgcag aaagacgtgt ggttctgcca
     9481 ttgccagcga gtggaattcc agatgatatt cctccagcat ggcacgaagc accttttcgt
     9541 tgaagctgct gaggtagagg atcccgatct cgctgtcgaa attgcggaca tcttccatga
     9601 tctcatgggt gcgggtttcc cgcagtgtga actcatagga ctccggcgag agccgctgga
     9661 tcagctgtac aaaggcatcg acagcaaagg catagtgctg tgtggaaatg gagcaccgct
     9721 gccgtgtctg tcgggtggca ccgtagtgct ggtggagcag gtccgcctgt tctaccacct
     9781 gtcgggcgta ggagaggaac tctctgccgt cctccgtgag ggcgatccct ctggcggaac
     9841 ggtggaaaat gagaatgccg aattcttctt ccagcgtgcg gacagcactg gaaagcgtcg
     9901 gctgtgccac gtaaagctgc ttggcagttt cgctgatgga gccgtgttcc acaatggcaa
     9961 tgacgtattg cagctgttgt aatgtcatga ccggtttcct caaatggttt cgatcatgcg
    10021 gagcacaatg tctgccacac gcttggcggc aattttctcg aactggtcga aagacattgc
    10081 tccctcatcg tcggcgttgt ccgagatgca gcggacgctg acgaacggca cctgattccg
    10141 gtatgcacac tgtcccacag ctgcactttc catatccacc gcatagggtt ccagccgctg
    10201 ctggatttca gccttgacag cactgtctgt gacaaaggct tcgccggaaa cgatacgtcc
    10261 tacaaaagac ttcacgccga actctgtgca ggtctttact gccagatcaa tcagagcctt
    10321 gtcagacgga tagatgccgc agtagggcgg gtagtctttc aggaagtgca ggtccagatc
    10381 atggggaaga acctcgctgg aaacgactac gtcgcagacg cggatctggt tgttcatgcc
    10441 gccggcgata ccggcattga taactgctgc cggctggaat gtgtcgatca gtgcctgcgt
    10501 gcagagggca gcgttgacct ttgcgatgcc gctgcaaaca tggacaatgc gggtgtcgcc
    10561 tacctgattg atgtagtagt caaagccggc ttttttctga atttccgaat ccggcagacc
    10621 gttgcgaatg tctgccagtt ccgacggcat agccccgatg attccgattg ttttcaacat
    10681 aaaaaattct ccttgttaat tggtgtgaaa ttgtgcctgc atctgaatcg gttctgcccg
    10741 atagaatgcg gagctgacca gtccgtccac gccggtggca gcatattcgg taacgttttc
    10801 ttctgtgagg tttccggcgg caactacggt gcagtgggga tatactgcct ttacctgttc
    10861 cactgctgct gccagtgcgt ccggctgcat ctggaaaaac tgaatggcat ctacgccgac
    10921 agacaaacag cggagagccg tttctgcgga agtggtttcc acaaagactt tcttttcaca
    10981 gaagtggctt ttctgctggg gataccgttc cagaaaaacg tccagaccgc ccatgcaggc
    11041 gatatgggtt tcgtagatca ggacagtttc cgacaagccc atgcggtgcg gaatcacacc
    11101 gccggcaacc actgccttgg tgaccagtgc ttttgtaccg gggaaatgtg cccgtgccgc
    11161 agtcagggaa atggcagggg aaatttcctg tacccgattg agcatgtttc tggctttggt
    11221 ggcaacgccg gaacagtggg aaagcaggga ctgacagatc ttccacgcac ggtgcaggtc
    11281 acagacgctg ccctctgcgg tgaagaacaa ctcgccggct ccgatctgtg tgccggtggg
    11341 gagggaatag gcggtggaaa gtcccagctt ggaaagaatg cgggaaacct cctcggtgca
    11401 gcagagcacg cagggttcgc gggtgtagaa agacaacgag cccggctcgt ccccgatgtc
    11461 cagccccagt gtggtcagat ccagatacgg ggtatcctgc tggatcaggg catccagttc
    11521 gctgtccgga atatagatca gatcgctacg cattgtgatc gctccttttt cattgtgatg
    11581 ctaccattat accacaaaac cgctgtgttg tccactgatt ttctttttcg tttcgggcgg
    11641 attgctttct tttcgaaatg aggcggtaaa aaggaacagg aaagttttgc aacaaatttt
    11701 aggactgtac tgcacaattg ccaaacggga gaaaatgtga tataatgatg atagattgtc
    11761 aagtcaagtg caacacaatc agagaaaaat agatttctga agaggtctta aggaagtata
    11821 gattgacaga aaggttattt atgaaaagca aaagaaaact acgatttctc atacctatat
    11881 tctgtatcgt tctatcactt gtcatctatc gtttggcgaa tggcttgatg agagaaagag
    11941 aatttcagca tatgccagaa atatatgaga taccgaatac ttcacaagtg tttacgcaaa
    12001 acaatgtcac gacagttcgg acgaattata tcataaaaag tgagtatacg gatttggatc
    12061 tgctgcatca aaaagtacag gattttattt atgataataa tatcatcaat acctcgatca
    12121 actgttcaga aacagaagaa tactatataa acttaagatt ctatcgcatt agtgaacagc
    12181 ttccttttga ttttgaacaa atatcgggat tcgggggaga tgtcattgaa aatcacactg
    12241 aagatttgat tgcaagagtt tctgtttatt ctgattctcc atatcaattg agttacaagt
    12301 tatggaatag aacagagaat gataaagatg gctataatga tgtgatagag gaaaggtctt
    12361 tttatgaagc actgcgaggc gttgcatcat gataatctga aacaacatgg aatttgaaaa
    12421 attcattttt acttttatcc caattctgtt ctgtatnnnn nnnnnntttt atactcactc
    12481 cgtatttttg ctgggtgagt tttttatttg atactttagg agttgattat gcggcaatgt
    12541 attaggaaaa tgcacaacgt ctatcttctt taattgacaa aaactgtgga aatatgctat
    12601 aataccaata gcacatctgg atatgtgtaa gtgcagttat gattgcagaa aggtgaaacg
    12661 accatgaaat atcgaaaact cacagacatc aatcaggaaa tgacagcctc cgagaacgcc
    12721 ttgcagcagg cagatcaggc atttctgggc gaactgcaaa aggtggcaga atcgatccgg
    12781 cagactgcgg cagagcgtcc ggtgatcctg ctggcaggtc cctctggttc gggaaagacc
    12841 acgacggcat ttttgctgga acggtttctg gatcgctggg gtgtggaaac gcatacgatt
    12901 tccatggaca atttcttttc cactatgaca ccgcagcagc gggaattgtc cgtcaagggc
    12961 aagctggatc tggaatcgcc ggcacgggta gatattccct atctgaatca gcagctgcaa
    13021 aaaatcattg cctgcgaacc cacatggttg ccgcggtatg aatttcccac aaccacgcgg
    13081 atcgatcagg actggcagct gacgcgaaag cccaacgaac tgttgctgct ggagggaatc
    13141 cacgccctga atccggaagt catcaccatt ccggaggaga tgaccgtgcg gatttacgtc
    13201 agtgtgcgga cgcgtgtgac caacggggaa actgtgctgc acccgtcacg tctgcggctg
    13261 atgcggcgaa tgctgcggga tcggaacttc cggcatcgca gcgtggcaga aactttgtcc
    13321 atgttcgaac acgtgcaggc aggggagcac aagtatattg ccccgtataa gaagcgggca
    13381 ttgttttcgg tagatacgtt tttgccttat gagctgggca tttataaaac cttgctggac
    13441 ggggaactgg aagcggaaat gcagcaccct cttctgcggg aattgcggca gatgtgggac
    13501 gacgtagaac cgctgcggct ggaacaggtg ccggcggatt cactggtgcg ggagttcgca
    13561 ggggatagtg cgtttcatta ttgaggggaa tcgattcggg tggaactgga aatttttgcg
    13621 gaagcgtagc ttctgagtct gccgtgtcaa aaccgtccgg cggaattcgc tcgtacctcg
    13681 ctgtccgcct gtggcttgcc aacgccgatt gttgcctttg cctgtatttg tgacgttgat
    13741 attcaggcgt tttctagtgt aggtgggtaa ctttatttca tacggcgttt gcaagtttgt
    13801 attgctaggc tcccctttag ggcacccgaa gggcgtgcct tgtgctgagc tggcaagccg
//
LOCUS       DAWBGR010000052        13612 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1418_length_13612_cov_5.007007, whole genome shotgun sequence.
ACCESSION   DAWBGR010000052 DAWBGR010000000
VERSION     DAWBGR010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13612)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13612)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13612
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1418_length_13612_cov_5.007007"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3014 a         3850 c         3341 g         3407 t
ORIGIN      
        1 gcactaagat aaattgcgac cggcagtatg cctgcggaat ttctatgcaa tctgctgaaa
       61 aatctgacta cgcatcttac gcacaagctt tattcagtgt ttccctaatt tcccgaaagg
      121 caactctata gcataaataa gcaatgccct ttccgcaaag gaaagggcat ttttgttgga
      181 aaccattatg ttccgcaaaa agtgcagtat tgcctttaca attttgtcgc ttcatggtat
      241 aataaaggca atctacacga aaacaaagga gcatgctatg agatccgcca gaactgcaaa
      301 acatatcatt atcggactgc tgctgttggg aattcttctc gtgggcgggt ggtatttcct
      361 atacccctac tcgctgggca gccgtatttc cgcaagcgaa cccctttctc tctcagtcaa
      421 cattacgggc gtgactgtca ccggaaaaga caccgtcgac acagtgacct atgaattcca
      481 gccggacacg caggagtaca aggcattggt atccctgctg aacgactacg actatcaccg
      541 cagcttcctc actctgcttc cgctggagga ctacacaccg gacagcaaca ccaccacatt
      601 ccagctgcaa agcacggaca gccagtatct gctgaccgac aagggcggct tctctgcgga
      661 gccggtttcc ggcggaaaat ctctcgtcta cgcaataggg tatctgggca gcggcaaggc
      721 ggaacagctt accgcggaat tgcaggcact gctgaaaagc tgcacgccgg tggaaacgca
      781 tgatgatacg gaaatgggaa cgacataatg caaacggcag ggaaccgatc aaagttccct
      841 gccgttttta aagcccgttt actgcaccgg cagcgtatcc accagatgca ccagaaattc
      901 catcagcaca gtggcatctg cactgtcaac ttccccgtcc ccattgcagt ccgcattctt
      961 ctttgccgct tcgttcagcg aaaccgttcc ggcactcgcc tttgcaagca gtactgcgtc
     1021 ctgaatgtca attctgccgt ccagatcgac atcaccgcaa agccattctt ccgacagtgc
     1081 cacaaaggga atctcgttct ctgccgcata ctgttccgca gtagaaccgg catagccata
     1141 gagcaccaga ttttcattgc ggttcggatc ggtttccagc cagacggaca catagcccag
     1201 tccggtatcc gtcaagtcta cgtcacggga gagaatggtc actttctcca gagtcgaatc
     1261 actttccaaa gcattgtcac cgatctcctc cacccctgcc ggaatggtga gttcttttgc
     1321 gccggcatca taaaatgcgt gatttccaat ggttttcagg ctgtccggca aggtgatctg
     1381 ggtacagtgg gtattctcaa atgtactgtc cccgatagaa accaagctgt ttcccagctg
     1441 taccgtttct gcatctgcaa gtccgagaaa tcccaatgcc acgtccagtt gtgtcaccgt
     1501 gtcggaaact gcaatgtgct tgatgccggt aaacgctgtc tgcactgcat aaacctccgg
     1561 catcagctct gcgagcactg cgttcttttc ctcgctgccc attgcagaag ggtcatagcc
     1621 atttgcctcc agcagttccg tcaattcgtt tgctgccgca ttcaaccatg catgatattc
     1681 ttcccaagga taaacaccct tttctatagt gcaggtatcc ccctgtatcg aaatctccac
     1741 accgtccgcc gttgcagatt cccatgtgca atgcaccgta tccccgtcaa ctgtccatgt
     1801 catggtatct cccaatgtgc cggtagcttc ctctgcggaa accggcagcc ctgccgttcc
     1861 ggtcagcatt gccaatgccg ccagaagtgc agtcattttc ttgcacgctt tcatacaatc
     1921 accctttctt gtaaatccct gcatagagcc gtctatgcat cgttccttgt ttttacatga
     1981 aatccggaac tgaaaaatcc aatttcatgt gattttgcac ttatatttca tattatatca
     2041 tatcagtatt gttttgtcaa ctttttttgc ggggagttca taaattcggc aaagcaacaa
     2101 aaaacgcacc acttccggtg cgtttttcta taggcaagtt gaaaagtacg cttatccctg
     2161 ccacggagca gcatagacgg aatccacgcc cagatattcc tccaatgtca ggtcatattt
     2221 ctggatctcc tttgcaatgt ccacacccac atagcggata tgccacggct catactgata
     2281 accggtgatg tcctcttttc ctttcggaaa acggataatg aagccatagt ccgcacaatg
     2341 ctgtgccagc cacgcggctt ccggcgtatc gccaaaggca tcgtcaatgg tgttgcagtc
     2401 aatggtcagt ccggtctggt gctcggaata tcccggacgg gcagaaaagc tgtccgcctg
     2461 ttccgaaccg tatatctcac agtaattgtt gtagatcttc acctgatagg catagtcccg
     2521 ataatcggaa ccaatataca gatccagccc ctcctgtgcc gccgcgtcac ttagcttctg
     2581 aaacgcgtcc ttggtaatag gctgcaagcc gggatcatag tcggcggaca gcggatagga
     2641 cttattaacg atcagaatgc cgtctacata ggttccctcc tgccgctgtt ccaactgccg
     2701 cacggcgatc tggtaagcag ttttgccgtc ggtgatctgg catctgccct gggttacgcc
     2761 ggtcactgtt ccattgctgt ccacggccgc caccgattca tcactactct tccactttcc
     2821 gacaggcaac tgcgccgtat tgccctccag caccgacaga aatgccggtg catcgatatt
     2881 agacggcgta tcgagagcat tggttccggc ggcattgttg ctgtcagaag tgctgttctg
     2941 caaggcagag gattctccgg cacggttcag tgtgttgggg atgaacaaga gccatacaga
     3001 aaaccccagc actgccagac agagcagcag tgcgatcagg tataactgtt tttgccgttt
     3061 ccggcggtgc tgttggtttc tgctcatatg aaacatcctt tctctgcacg ttattgtgcg
     3121 cgatagatca ctgtgctgtc agtgagatca tagccgctca gtccgctggt gccgtcagaa
     3181 gcttcgccca caaaagcaaa gcgatatgcg gtgatcgtct tcacctctga cggatcgatg
     3241 ggttcgctgt agcaaagcac atctgcggcg ctgtccgcat cccaatagac ctctgtcagc
     3301 ggcacatact gcccgatggt gcctgttccg cagccgggca gcgcatggat ccgccgcagc
     3361 atctcctcac cttctgcatt ctgtaccaac tcatcaaaaa tctttctgcc gtctttcagc
     3421 gtgatgacat aatcgactgt gtggctctcg tcatcccatt ctgccggagc tgtgatcgtt
     3481 gccgaaagtt cctgcaagtt tactgtgcct ccgtcaaaat cagctgtgat gggtgctgca
     3541 ctggcaaaat ccaatgtaac gctagtgtgc cagttgccgg aaactacacc ctctgtctct
     3601 gcatagagtt ccttttcccg gtcgctgcac ttctgcgcca gttggttttc ccggcgataa
     3661 gctttccaag cgtttacatc aaaacctgcc ggaagatcgc cgtcccccca gtctttcaac
     3721 gcatcttccc cgaagttctg ccggacatac tcctgtagga actggtcgtc ataggctgtt
     3781 agctgttccc agatctgaga gacctgtgcc tctgtataga aacttccaaa agtcacatcc
     3841 agtgtttttc cctgcaaatc cgtgtggttg atacgaaggt tgatataata cgctccgtcc
     3901 ttttcccgga catctaccgt tctgccataa cagtccagtg tctcactgcc gccgtctgca
     3961 tccagcaaga gcaattgata gggcagtgcc aagccgtcct gcaacgccgc accgtccatg
     4021 tcagtcacct gaaagctcag cattgcctgg ctgtcatcgc cgtacatgcc cagcagctta
     4081 aactgtacat tctcgttggt gctgtcgaag tccgctgctg acgtagtgac cagagccgcc
     4141 agcttttccg gatctttcgg tgtttcttca ccgatcaggc tctggaagaa tgcgtccagt
     4201 ccgccgtagc cggctgctgc taccagcgtt gtgccgccag tgaccagcac tgccgctgcg
     4261 gcgatgcctg ccacccactt ggttatgtgc atgcgattcc gcttcggctg cggagcaatg
     4321 ccagtttgtt cctgcactcg tttccaaatt gcatcggtat gaatcggctg ggtttccagc
     4381 ttcggcacac tgtttttttc tgctgcatag tggtcaaacc aatcaaagca attccatttc
     4441 atgagatagc tcctttcccc atggcggtga agcgaacctg ctgcactgcc ggtgtgtctt
     4501 catacgcata tccccgttcc accagttcct gctgcaactt ctttctggca cggctcattc
     4561 gcattttcac tgcggattcg ctcatgtgca gcgtgccggc aatctgccgg acgggttcat
     4621 agcaatagta gtaccgcacc agaatctccc tgtcctttgc ggagagggtg tccagtacgt
     4681 cctgtaacag ttcccccaga atcttctggt cgatgctgcc ttctacatct tcttcgctgc
     4741 cgatctccac cagatcttcc agattcacgg tttcgtgata gctgcggatt cggttcttcg
     4801 cccgatttcg ggcaattgcc gccagatatg ccgtcagctt tccggctttc agctggtcgg
     4861 tatgttccca caccgccaga aacacatctg ccgtcagttc ttccacgtcc ggttctgcca
     4921 gatactttcc gataatattg cgtatgatcg ttcccacata ggggctataa gttgtaatca
     4981 actcctccag agcctgcggc tggtgctgtt tgagtctgcg tatcagagaa cgttcctgca
     5041 cgctggttca ctcctttcgt atgttccgaa cggccgttca cctataacaa cacgcattcc
     5101 gcagaaatgg tcacatgatt ggtgtttccc tattcctcgt ctttcttttc gtcctctgct
     5161 gccggcatat cggcgtgacc gatatggtgc agctggctgt gtgaaatggt ctgctcatag
     5221 tctgccactt cttccgcctc gtcctcgtcg tcatcgtcca gaatgctgcg gtaaccgtca
     5281 tcgtattccg gtttctcctc tgcagcttcg tcctgcttgc cacgctggga gagcgggatc
     5341 ttgtgctcag cttcggcttc ctgcaaggct ttcttttcct ctgctgtcat catgttccgg
     5401 cggatcaggc gaattgcgat cagcagcact gccatcaaaa ccaccgccag tatgccccac
     5461 agcataacag ctttccagtt gacgttcatt gccttgaagc tgatcttcac gtcgcagtcc
     5521 ttgacataat ctgatgctgc accggtatag ccgatcagca ggaagccgtc cagagccttg
     5581 actttcagat tgtcgccgga gtctgacgaa actgtcacat agtcctcgct gtcgtcatca
     5641 tcagcaggag aggtgcagcc aaaagcgtac ttgcccacag aggaaacccg cttcggcagc
     5701 tgaacttctt tcaggttgtc gtcacagaaa aaggcgtagt ccccgatttc caaatcgccg
     5761 ccctcgccgc cgtcctcgtc agaggcaccg gaaaaagtga cagaagtcag accagtacag
     5821 tcggcaaatg cagtcgttcc aatggtggta actgctttcg gaatagtcag gctggtcagc
     5881 ccgctgcaaa aatcgaatgc catttcgccg atgtactgta atttctcatc aaagttgatc
     5941 tgctggaggt tgcctgctgc aaagaacgca ccggcataga ccaccagtgt gctttccggc
     6001 aaagtaaagc tggtatccgt ccgccctgcg gggtagagat acagaatgct ttcctctgta
     6061 tcatacagca cgttgtccac tgcctgatat ttggtgttgc cgtcctctac cgcaatggtt
     6121 tccagcgacg ggcatgccac aaaggacatg gctccgatat tctccagatt cttcggaacg
     6181 gtaaaggact tcagggacat gcagccgtaa aaggtatagc cacccagttc cgtcaccttt
     6241 tccggaatct gcacttcgtc cagagccgtg cagtagtaga acagctggtc gcccagtgtt
     6301 tccactgtat cgggcagcgt caccgtttcc agactggtac agtagccgaa tgccatatcg
     6361 ccgatctccg tggtgtcctt gcccagcgtc aggtcagtca gtgcggtaca gctgaaaaat
     6421 gtgccctgct cgattttggt cacagcgtcc ggtacagtca ctttggtcag tgcggtacag
     6481 ccgtaaaagg cagccgaacc gatctcggtc acactggcgg gaatggtcac actttgcagc
     6541 gacgtgcagt tggcgaaagc ttcttccccg atcgatacaa tgtcatagcc gtcgattttc
     6601 ggcatgatac tgatatgggt cgccgactcc acacagtcag tgacagaaac tgtggtattg
     6661 ctcagctttt tataggtcag cgtgccgtca tcgaacgtgt cgctgctctc ggcagtagtg
     6721 gtttctgccg cccataccac gccgccggca ggcagcagag cacagacagc aaccgatgct
     6781 gcggcaaggg cagcagcacc gcgtttgaat gcaaatggaa atgtagactt catggatttc
     6841 gggttccttt ctgcggcaag tgtaccggta tcactttttc tttgttgccg aggcatgccg
     6901 actggtaggc cggatcttat tccgactctg gtgcacagcg atcagcgtaa caataccgcc
     6961 ggcaacgact acagtgatcc cgccgccgat cagccagtat ttataggtgt ttgctctcca
     7021 gtgctgcatg tacttccgca ccgcttcgct gccgtcgttg tacacgtaca gctggtcgat
     7081 ctcttttgcc tcgttgtctt tggcatcgga cggcgtatag tatccgattg cctgttcccc
     7141 gatctctgta atgcttttcg gcagattcat ctgcaatagt gccacacagt tatagaagca
     7201 ctggtcgccg atcgtggtca gaccgtccgg cagttccagt tttttcagct tggcacagga
     7261 atagaagcaa tgattcccca attttcgtat ggtggacggc agtgttgcct cctccatgga
     7321 caaacagtag gcaaatactg ccccgttcag ttctgtgatg ccctccggca cagtgatgct
     7381 tttcagagcc gtgcagtaat agaagcagtc ctctccgatc gcagtcaccc cgtcaatgtt
     7441 gatctgctcc aatgctgtag agccgataaa gctccagtcc gccagcgtgg tacagccctc
     7501 cggcacggtg tagctggttt ccgcctttgc ctgcggatag cggagcagcg tttctccgtc
     7561 tgcggtaaac agcacgccgt cctgctcctg atatgccgga ttttccggtg caacggaaaa
     7621 tgccgtcagt gcctgacagc cgtcaaacac ataatttccc agcgtggtgc aggaggcagg
     7681 cagggaaacg gtttccagca cctgacagtt ctggaacgca tagtccccga tgctgacaat
     7741 gccctcggac agggtcactt cctgtagggc agtgcagctg tagaacgcct cgctgccaat
     7801 ggtctggata ttggcgggca ccgtaaagct gggaaaatag ctgcaattgt agaacgccgc
     7861 ctccggcagt tctgtcagcg tggagggaat gttcacctca ctcagcatgg tgcaggattc
     7921 aaaagtaccc tttcccagtt cccgcagtcc ctccggcaaa gacactgtgc acagcatggg
     7981 acagtccgaa aaggcacttt ccccgatttt tgtcacgtgg gacggaatcg tcagcgaggt
     8041 caggaactga cagttcataa aggcggcatc accgatctct gtcaccggtt tcccgtcgat
     8101 ctcctccggc aggttcgctg ctgtggcaga agtgtcacag gaagtaatgg tcacgccggt
     8161 gtcattttcc cgataggtga acacatcata ggcagcatcg ccggatgtgt ccacagaaat
     8221 gccgctgtca tcttctgttg cattctccgt tgtcattgcc gccgctgccg gcactgcacc
     8281 cagcagtccg acacacagcg tggttcccaa aagtccgcaa atcagctgtt tccacgttct
     8341 catttggtat cctcatggtc tgccgcttcc ggcttttctt tactttccac agactgggta
     8401 tcctttttgg ctgccttgcc ggatttcttc tgttttccgc cgccggcaaa gatcagcact
     8461 ccgccgccga tgagcagtac cagtgcaccg gctcccagca ggatccactt gccgtatttc
     8521 tgccagccgc tttcggtttc ctcaacagca acagccgtat tctccgtatc cgaaacttcc
     8581 tcggacatca cgctgcggaa tttgaagttg ttggaatagt cgttttcaga gtcctctgcg
     8641 gtgcagtatg cctctgcctg tgaaccgacc ttgccgtaga tcacgaagtc ctgtaccttg
     8701 gtcaccatat cgtcttcata gccgaatgca cagaatccaa tggtggaaac gctctccgga
     8761 atggtcactt ctttcagacc ggtgccggca aaggctgcca tttgaatatc cgtcaggctg
     8821 tcgtaaaatg tcaccttggt caggctggtt tcgccggcaa aggctgccgc ctgaatcagt
     8881 tccagatttt tcggcatgtc gatttccgaa atgccagtgc agtatgcaaa ggcgtactgt
     8941 ccgatttccg tgacagtatc cggcagatcc agagaagtca gtgccgagga ggcaaatgcc
     9001 cagtcgtcaa tgtacagcag tccgtccgga aatgtcacgt cagtcagctt ggtgttggaa
     9061 aaagcagagg tccagatctc tctgacggtg tccggaatgg tataggacgt gccctccatt
     9121 gcctgcggat agacgatcag atcttctccg tttgcggaaa acaatacgcc gtccgtcacc
     9181 gtaaacgccg tgttgccctc tgccagcgac acctgttcca gcacggtaca gccgtagaag
     9241 caggaactgt tgatcgtgtc aaccgttgcc ggaatggtaa tcgaagtgat catatccttg
     9301 gtggcaaatg tgccgctgcc cagtgtggta acggtgtgcc cgtccagctt gtccggtatc
     9361 gtaacggcag tttcgctgcc gttatacttg gtaatggtgg cgttgtcacc gtccagcgta
     9421 taggaataat ccccgctggt cagcgtttct ttgctgctct catcgctgga taagctgtct
     9481 gcgtcgatca cgctttcttc gctgctctgg gcagaggttt cttcctctgc cgcagccgac
     9541 agtcccagtg cctgtgtcat catgacgact gccgcagtca gcagcacgat tttggtatgt
     9601 ttcatgtttt tcccgttcct tttctattat tccactgtca cggactttgc cagattccgc
     9661 ggcttatcta catcattgcc cttgagcaca gaagtatagt atgcgatcag ctgcaacggt
     9721 accacagcga tcatgggcat cagcaggtcg tcaaattccg gcagaccaaa cttcaggtct
     9781 actgcgtccg gatccacatt gtcgctctca cggcacacca gaatgacccg tgcaccacgt
     9841 gccttgactt ccttgatgtt gctgatggtc ttttcccgca gtgccttctg ggtcgctacg
     9901 gcaatgaccg gcatgttttc cgtaatcagc gaaatggtgc cgtgcttcaa ttctccggct
     9961 gcataagact ccgagtggat ataggagatt tctttcagct tcagcgagcc ttccatgctc
    10021 agggcgtagt ccagtccgcg tccgatgtac agcaggctgt cggcggtgat gagggaggag
    10081 gcaatatact gtgtctgctc cttatgttcc agaatgccgg taatcacttc cggcgtttcc
    10141 tgcaaaagct ttaccagacg gcggcactct gtttcgtcaa tgttgcccac tgccagagcc
    10201 agttcaaagg caaacagata catcaccgcg atctgcacca tgtacgcctt ggtcgatgcc
    10261 acggcgattt ccggtccggc atgggtgtag atcagcatat ctgcttcccg tgcaatggaa
    10321 ctgcccattg cgttgacaat ggcgagcgtc tttgccccca gcgatttcgc cagccgcaga
    10381 gctgccagac tgtcggcagt ttcgccggac tgggagatga tgatgaccag atcgccttct
    10441 cccaccagcg gttcccgata gcggaattcc gaagcaatgt ccaccgttac cggcacccgt
    10501 gccagctttt caatgacata cttgcccact gttccggcgt gcattgccgt accgcaggca
    10561 actacctgaa tgtgccggaa ttcccgcagc acctccggtg tgatgccgca ttcctccaga
    10621 ttcggcaagc cgtcctgaat ccgcggcaga atggtgttgc gaatcgcgtt gggctgttcg
    10681 tggatctctt tcagcataaa gtgggcatag ccgcccttgc cgatcgcctc cacgtcccag
    10741 tctgcggttt tcagttcctt tttcaccggc tgcccgaacg cgtcgaagat ctgtacgccg
    10801 ttgtcgtcca gcacggcaat ttcctcgtgt tccagcaggt agtaatctct ggtgtattcg
    10861 atgattgccg gcacatcaga ggcaataaag ctttcgccct tgccgacacc cacgatcagc
    10921 gggctttcgc agcggacagc gtacacctgc tcggggtgat ctgcgaagat aatgcccagc
    10981 gaataggaac cttccagatc gcggacggtt tccgaaatgg tctgaatggg gctgccgtta
    11041 tagcgatagt ccagcagctt tgccaccact tcggtgtcag tgtcgctggt aaaggtcacg
    11101 ccttttcccg caaggaagtc cttgagttcc ttgtagttct cgatgatgcc gttatgtacg
    11161 atcgtcactc tgccgccgct gtgggggtga gcgtttacgt cagagggttc gccgtgggtc
    11221 gcccagcggg tgtggccaat gcccacatgc ccgaaaggtc tgccctcacg ctccattttt
    11281 tcctccagat ctttcagtct gcccttagac ttcgccacag tgatttttcc gtcctgaaaa
    11341 acggcaatgc cggcagagtc atagccgcgg tattccagtt ttcccagacc gttcagcagg
    11401 acctccgtac agtcccgttt gcccacatat ccaacaattc cacacatctt ttgtgcctca
    11461 ctttcgtttt catggcagct ccgcaccaaa ccggtgcgaa gtcgcttcat atgcatcgtg
    11521 tcccaaatga ccggacacta catattatgg cagcactgca cctagtctgc ctgcgaaatt
    11581 tctatgcaat ctgccaaaaa atctgacgat gcaccttacg cacaagcttt gcacggtgtt
    11641 gccttatcaa ttataccata accgcactgg aattgcaact gattttttag tgaaaattac
    11701 gtgatatggg tttgtgaaac aaagggaatt ccggcaattc gacagcaaaa acaacttttt
    11761 cgattctgcg gaattttcaa aaaatcgccg aatgcagttg cagtctgtcg aaaaatgtga
    11821 tatactaagg gcaatactgc acaaagagcg tctggcgaaa aatttgacga cgcatctgtc
    11881 gcacaatctt tgtgcggtat tgcctaaggg aaacgccgaa tcagggaaac actgtataaa
    11941 acgaaaagag gtcatgtccc atatgaatca aaatcaagta aatctcgagc cggaagaacc
    12001 gcttctgccg agtccgtctt cggaaatgct cgcttacatg gaaaaactct atccccttat
    12061 ctttgccttt aagcagacaa aactgtggaa atccatacgg gattccgagc cgtttgcgat
    12121 ccagatgccc gacggacaaa ccggctgctg cattctgacc ggaaatgagg gggattttat
    12181 ttccctgtcc atttattttg gtgatgccgg ctggaacagc atctgcaatt tctacagcga
    12241 accggtagaa acggaagaag ctcgcctgga acagatgatg tcccaatcct gtgtacaggt
    12301 agtattctgc cgcaaagacg gactgacaga aacggaagtg caaagcctgc ggcactacgc
    12361 cagagaaaac aacatcgcat tccgcggcaa aaacgcctac ttctgcctgc ggaactccga
    12421 gccctaccac tgctcatggt ttctggaaac caaagaacag gcagacatac tggaactggg
    12481 gcttcgtgca gcactggatc tccataagca gctgggacag ggaaaaacac cggctgaact
    12541 gggcatcagt tcctgcatcg acacactgcc gctgtatgac ggtaagacat ggagcaccat
    12601 gatgccgccg gaacgaaccg tatcttcttt gacggaacct ttgctgagcg atgccatgca
    12661 gcagaagatc cgccgcatga aaaagggcgg cggcaagtgg gaatgccagc tgtttccatt
    12721 ttcaagtcct gtgctcgcca aagagggtgc accgtttttc ccgttcctgc tgatgattct
    12781 ggacaccaag cgggagcagc ttctgcccca gcagccacta cagtacaacg cagatattct
    12841 ccaaaaggca ctggacagct tcgtacaggc aattatcgac tatggtcgct gccccaagag
    12901 cattgccgtc tgcgacgagc gaaccgaagc gatcctctcg gagctgtgtc acaaatgcgg
    12961 cattctcctg ctgaagacca cccgttttgc catgctggac gaggcgaagg catcttttct
    13021 ggaggatatg gaagcagaaa aacaggcaga cgatgccgag tcggaaaaaa tgatcgccgc
    13081 atttgtagag catctgaaag acatgactgt ggaggagatc aaggacgatg ctccgctgga
    13141 cgtgatccgc tggctcatgg tgtcggacat tccggaggat ctgatgaaaa agctggagaa
    13201 agtcatggag gaactgcagt aatatgaacc agaccattta ccgcgtcctg cacatttacg
    13261 agagcgacta tggctgtgag gagcatgcag agcctaccgc agaggtagtg ctggaagcgg
    13321 cggacggcag ccggaaaacc atttcctatt ccgatgccgc actttacaca ctggacattt
    13381 tcgagggtga cagcgttacg tatacagacg gcacgctgca aaaagtcatc tcggaacagg
    13441 attcgtaatc agaaagacaa cactacacca agggcgaacc gaaatcgtat cggttcgccc
    13501 tttttgcttt atgcggtgtt gcctttaggc aataccgcac aaagagcgtc tggcgaattt
    13561 gcacgccatc tgcttgcaaa tttcccgtca tatttcacaa aaatgctcgt ga
//
LOCUS       DAWBGR010000053        13546 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1424_length_13546_cov_3.331184, whole genome shotgun sequence.
ACCESSION   DAWBGR010000053 DAWBGR010000000
VERSION     DAWBGR010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13546)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13546)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13546
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1424_length_13546_cov_3.331184"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4123 a         2729 c         3112 g         3582 t
ORIGIN      
        1 ggcacttaaa tgacctacga acaaatcgca gaaatgatgg aagaaatggg gctgcctttt
       61 gcttaccacc atttcgctga gggcgaaagt ccagcacctc cttttttact atttttatct
      121 cccggagaga atacattttc tgcggataat cagatgtatt tcagctttaa acagctggat
      181 attgaattat atacagacat taagaatcct gaactggaaa agcagataga acaggttctg
      241 aaacgtcata aaatctacta tacaaaatca gaagtatgga tagagtccga aaaactctat
      301 gaagtacttt acgaaacgga ggtataactt atggcaaaca aaaagaataa ggttaaattc
      361 ggtttgcaga atgtgtactg ggcaaagatt aacgaatggg gtgtagactc cgatggcaac
      421 aggactttcc ctgcatatgg agagtcaaag catctgccgg gtgctgtatc gttatctatt
      481 gatgcaaacg gcgaggccga gaatttttac gcggacaacg gtgtatatta cgtgattaac
      541 aacaacgccg gctacaccgg tgaccttgaa attgccctta tcacaaccga atttgcgacg
      601 gaaatccttg gagaaattct cgacaacaac ggcgttcttg tggaaagaaa tgatacagaa
      661 cttgcacagt ttgcattgat gtttgagttt cttggagaca agcaccacat cagacacgtg
      721 atgtattgct gctcagcttc acgtcctgcg acagaatctg caactacaga ggaaagcaca
      781 gaagttaaga cggaaaagct gacgctcaag gcgacgcctt tgccgacggg tcttgtaaag
      841 tccaagacaa ctgaaagtac atcggataca gtttacaaca actggttcaa gacaccctac
      901 agcccaagta cacaaacagc cacaacaact accaagacat cttaaggagg tactgctatg
      961 tctatcaaga agaatattac cattgacggt atggaggtac cattcaaggc aagtgcagct
     1021 gtgccacgtc tttatcgtct gaagttccac agggatattt acaaggactt tgcatcgctg
     1081 aaaacggatg tggaagaggg agatgagaac aaaagcgaac tcgatattga gagccttgag
     1141 gttttcgaga acatcgccta catcatggca aaacacgccg acccggaaaa cgttcccgac
     1201 agccctgatg atttcctgga acggttcaat acgtttagta tttatgagat tcttcctcag
     1261 ctgattgaat tgtggggact gaatacagca acacaggttg agtctaaaaa aaacatcgcc
     1321 cgactgaccg gccgatgact actccgcttt ttctcctgag atgcaaacag ctcggtcttt
     1381 ccatgaccga gctggatttg ctgacaattg ggcttatcaa tgatatgttc acggaacgtg
     1441 aaaatgatga ttacgatggc tggaatgagg tcgctggaca ggcggatttt gataatttct
     1501 aaatagcctc tttacttttt tctctttatg cagtataata aaggaaaaga ttctaagggg
     1561 tggaaatatg agtaatttta gagatgaaga ttatgcttat attcttggag attgtctgca
     1621 tgatattttt tatgttgatt catcttaccg tggaaaaatt gctcaaatga ggttgctttc
     1681 tgaaataata gtaaggaagc tgatcgatta taatccagat gatcagttga caatcggtga
     1741 caaggaagtc ctaaagactg taaaagctct cacatacgga gaccatttta agaaatgtat
     1801 tcttgctgtt aagaatgata caactgacta ttacgcagcg aattcctgtt cacattcaaa
     1861 agtacgttcg caaataacac aagatgatta taacaagatc tatgagcatt tacttgatct
     1921 tatttcttgc ttatttattc agttcttttc caagcatact tttggcacga accaaaagat
     1981 agttagctgt ttttctttgc tcccgcctat tattcgatac aaggtgctga gttttttata
     2041 tgctaatgac aaccataaca ttattttgat cgataaatta gtgttagtaa cattaaaaga
     2101 atttgggaag gaaaagacga ctcaatggct tgaagacaat aaagctcatt tgacttcaat
     2161 tccaactgat ttttatgata atatgtatca atactctcta cgaacagtgg caacaaacat
     2221 gaaagcaaca tatcaaacaa tggaagaggc aaaggtgttc ttcaatcaca ataaaaaagc
     2281 ttttgttgag gacaccgagg aagaggtgcg agactttgcc aaattgatgg aatttttcta
     2341 taccggaaga aagctggaca caaaaatagt tccgtcagaa tatgttgtga gctttcatga
     2401 caaaaaataa ctgccattta tgtaaggagg aatgatttgt atgccgtaca atgctttagg
     2461 tgatctgtat aagatcgaag ttgtcaaaag gcttcaaaaa atgggatgca atgtcaaaag
     2521 tgttcatgcc ttaaatctga ttttagagaa aatgggcatc cttattcatt ctggagatca
     2581 ttggctcact tcaaaaaacg gggttaagta caccatatac agcagtcaag tattcgatgc
     2641 tgatgcgtgg cacccttcaa ttgttgatgc tgttcttgaa tatttacaaa atagcggaag
     2701 agcctaaaat attaaataca cacaaagcac ttgctacggc aggggctttt ttcatgcctt
     2761 tttgcaggag gtgaaaccac atggcaaaca gaatcaaagg cattacagtt gaaatcggcg
     2821 gtgataccac caaactttcc aaggctctgg aaggtgttaa caagaacatc aagagtactc
     2881 agacgcagct gaaggacgtg gaaaagcttc tgaagcttga cccgaaaaat acagaactgc
     2941 tttcccagaa acaaaagctg cttgctgaca gcattgctac taccaaggag aaactcacca
     3001 cgctgaaaac tgccgcagaa caagccaata ctgcactcgc caatggtgaa atctctcagg
     3061 aacagtatga cgcattacag cgagaaatta tcgaaaccga acaggaactc cgcaatctcg
     3121 aaaccgaagc cggcaaagca tcggactctt tgaagcaaat cggtgaagcc ggagagattc
     3181 tccagaatgt cggggacaag attttcgatg ttggtgctac acttaccaca aaggttacag
     3241 ttccgattgc cgccgcaggt acagcagcag tcaagacagc atctgacttt gattctgcta
     3301 tgtcgaaggt cgcagctgta tccggtgcaa ccggtgatga ccttgaaaag cttcgtgata
     3361 aggctcgtga aatgggttcg aaaacgaagt tctcagcatc ggaagctgct gaagcaatga
     3421 actacatggc tatggcaggc tggaaaaccg gcgatatgct ttccggtatt gacggtatca
     3481 tgaaccttgc ggctgcgagt ggtgaggatt tggcaaccac atcggatatt gtcactgatg
     3541 cgcttactgc attcggctta tccgctgctg acagcggtca ttttgccgat gttctcgctt
     3601 ctgcaagttc caacgccaat accaatgtat ctatgcttgg tgaatccttc aagtactgtg
     3661 ctccgattgc gggtgctttg ggattctcct gtgaagatac agccgaggca ttaggcttaa
     3721 tggcaaacgc aggcatcaag tccacacagt ccggtacttc catgcgttcc attatgaccg
     3781 cactttccag tgaggtcaaa ttctgctcct cttctttcgg agaaatggag attgcaacca
     3841 gcaatgcgga cggctccatg cgtgaccttt ccgatattct tgccgactgc cgagtggcat
     3901 ttgaccagat gtctgaatct gaaaaagcaa gcgcagcaca ggctcttgtg ggcaagaatg
     3961 ccatgtcagg ctttttggct ttgatgaatg ctgcacccca ggacgttgaa aagctgtcat
     4021 ctgcgattgc gaactgtgac ggcacatcgc tttctatggc ggaaacaatg caggacaacc
     4081 ttggcggtca gctgactatc ttaaaatcac agctggaaga actggctatt tctttcggcg
     4141 aaattctgat gcctgtcatc agatcaatcg taacgaaaat tcaggaattt attgataaac
     4201 tcaacgccat ggaccctgcc acaaaggaga cgattgtgaa agttgctctc gttgctgcgg
     4261 caatgggtcc tttattggtg gtaatcggca aagtcatatc ctcggtggga agtctgatga
     4321 cttttatcag caaagttccg actataattg caggtgcaaa gacagcattt tccactcttg
     4381 gagctgccat tggcggtatt tctgcaccgg tggtggctgt gattgcaatc gttgctgtat
     4441 tagttgccgc ctttgtaaat ctgtggaata ccaatgagga cttcaaaaat agcattctct
     4501 ccatttggga acagataaaa gctacatttg aaaggcttac ttccggaatc gtcgaccgca
     4561 tcaatgctct cggctttgac tttgagagct tcggagaact ggtgaaagcc gtgtggaatg
     4621 gactttgtga agttcttgcc ccactgtttg agggcgtatt tcagcacatt gcagatattt
     4681 tttctttcgt aaccgacacc attttaagta ttctcgacat tttcatcggt ctgtttaccg
     4741 gagattggga tcagtgctgg aatggcataa aagacctctt tacaggcata tgggatttca
     4801 ttgtaaactc actcagcaat attctgaaca cgctgacagg tgtgttagat gtattccttg
     4861 gctggttcgg cacttcatgg gacgaggtct ggacggcaat caaggatttc ttcattggca
     4921 tatgggacag catttgctct ttcttccagt ccattgccga tttctttgtg aacacctgga
     4981 atgcgatatc ttcattcttt acgggaattg taactgctat tcacgatact gcggtttcta
     5041 tttttacggc tgtttatgac tttttcgcag gaatcctgac aagcattcac gatttcttct
     5101 ccaccatttt caatgccata tggacggtca tttctacggt atgcaccaca atctacaata
     5161 cgatttcaag catatggaat acgatatatg agttcatatc tccgcttttg gaagccttga
     5221 aatatctgtt tgagacgatt tttcaggcaa tacatatcat tatcagcaat gtgatggact
     5281 ggatttctga gaagatacag accatatgga atgccattgt ggagtttatc acccctctgc
     5341 ttgaaggcat taagtcattc tttgaaacca tctggaatgc aatcagtact gcgatttcca
     5401 cagtgatgag tacgatttca aatatcatca ccacggtatg gaacgcaatt tcaggcttta
     5461 tttcaagtgt gatgaacacc atcaaatcgg taatttcctc catctggaac accatcagcg
     5521 gtgcgatttc aggtgttgta aacggaatca gaaatacaat atcttccgtt tggaacagca
     5581 tttcttctac aatttcatcg gtgatgaata ccatacgttc tactgtgaca agcatctgga
     5641 acagcgtaaa atcagcgatt tccaatacaa tcagcggtat ttacgatacg attaagagtg
     5701 gatttgacaa ggcggtcaac tttgtaaagg gactggcgag tgatgcattc agctggggtt
     5761 cggatatcat cagcggaatt gttgacggta tcaaaagctg tatcagctgg atttccgacg
     5821 cctgtaccga tgtggcggat accatcagaa gctatctgca cttctctgtg ccggacgtgg
     5881 gacctcttac cgaatacgaa agctggatgc cggactttat gcaggggtta gcagacggca
     5941 ttatcaaaag caaaaaggtt attgcaaagg cggtatccgg tgttgcggat acaatgaaaa
     6001 ttgctcttaa taccgacctt agctacaaac ttgacggaat gacaggtgct atcatgaacg
     6061 gcggaattga aagttctgtg gtcaataact actacaataa cgacaacagc cgtacagtaa
     6121 atcagaccaa caatagtccg aaatcactgt cacggctgga gatttataga cagacaaaga
     6181 atgcggtgaa gatgtagaaa ggagtgattc cagatgttct atcacctaat cctggaaaac
     6241 gaaaccggtc agcaaattaa cctgtccaga acagcaaaca ggttcatgtt ctcaaagatt
     6301 gaaggactga atccacctgc cggaacagtc agcacatcaa gctatgccgg aatggacggt
     6361 tcttacctca acaatgcttt cattgaaaag cgaaacgtgg tcattccttt tgaaatgcgt
     6421 ggctttgatg tggagaaacg caggcatgag ctgtatcagg tggtcaagcc gtcacgctac
     6481 atcaaaatat attactccac aaaaaacatt tctgtgtatg ccgagggtat tgttgaaacc
     6541 tgcgaaatgg agaactttga gatgctgacc aaagggcaga tatctattct ctgtcccgat
     6601 atttactggt attccacgga aacccagatt gcggagtatt ccaaaatccg tggtgctttc
     6661 cacttcatct tccccgataa tgatgagccg tttccaatcg gtcaatacag cacacaaaac
     6721 atcatgacca ttgtcaatga cggtgatgaa gtgggcttta tccttgaaat cagcggaggt
     6781 cctgcaaaga atccgactat ttacaatgca gctacagacg aatatatgca gattctcggt
     6841 gacatcaaag acggcgatgt tatcaccata actacgaaaa caggcaataa gacggttaca
     6901 ctggaacgtg agggtgttgt tactaatatt atcaaccggc ttgtttcggg ttccacctgg
     6961 ctgaccttaa agcagggaga aaataagttt tatgttcgtg cgtctgaggg actgtcaagt
     7021 cttaaagtcc gtctgataca ccgcaatgcg tacttgggag tgtgaaaaat gcaaattgaa
     7081 atatacaaca tgattcccat agaggacaaa ctctccataa ccctggaggc tgtgtgtgac
     7141 agcttttctt cgctgctttg ggatattgaa tactatgcct gcggtgtatt tgaagtgtac
     7201 attgcggcat ctacgaaaaa tattgaaatc ttccggacag gcagaattgt gggtcgtgat
     7261 gatgataaag aacactacgg actgattgaa tcggtacagc tggaaacaga tgcagaggac
     7321 ggcgattatc tcattgtgag cggtcgcttt ttaatgtgtc ttctggaaag aagaatcatt
     7381 tgtcctacat tcaactttac aaagaaagtt tcatacgcac agattgtgaa caatgtggtt
     7441 tattacaacg cctgcagaac aggtgcgaga aagattccgg ggctttcgat tggtgattct
     7501 tccggttctt gctggaaaca agatactaaa ctgcaaatca gctacgataa ccttatgaaa
     7561 tgggtataca ccatctgtga gaaaatcggt gggactgcca atatacgact ggcaaagaca
     7621 accgatgaac agtatgaaat gctgctggaa ttgtcagagg gtacagatag aagtatctta
     7681 caagatgata acccacatat tgttttctca gatggataca acaatctgct ttcattttcc
     7741 tattccacag atagttctgt gcagagaaat tatgcctata tattgggtaa aggcgaagga
     7801 gaagagagaa aacgaaccac atattgtgac ggcgatgaac cggaacacct tgaccgctat
     7861 gaggtgtatg ttgacgcaaa ggatatggcg gacgaagaac aggaagacgg cgaaaccaag
     7921 ccaattccca atgatgaata cattaacctt ttacaggaaa aaggcaaaga aagtatggtg
     7981 cagccacttg tggtttccga atcgcagatt gcggtacagt ctacgcagtt tcagtataac
     8041 agggactatt ttgttggcga ttttgttacc gtagaacacc gcagatttgg tctgagacag
     8101 aataaaatac agctgatcgg tatgattgaa agcttcgacc agaacggaag aaatctcaca
     8161 cccacattca aggaggtatg actatggcat tttcatatgg atttttcaac gcaaagaatc
     8221 ttgacagaac gtatactgcc gagaatttct gcgattatct tggcagtatc atctgcaacg
     8281 gcattcagga taactacggt cagtgtttca agctgacagc aaacaagcta aagctgacca
     8341 ttggcagcgg taaggcgtgg ataaacggac attactttct ttccgatacg ccgtatacct
     8401 atgatttatc aggctatgtg aatgaatctc tgggcagata tttatcagtg ggtatctgct
     8461 gtaataccgg cgaaaattat cgtaagatag agtttgaaat tctatccggt acgcctgcca
     8521 catcgccgtc aattccaaga ttcaaagata cagaaaccaa aacatatctc accctttgtg
     8581 ccgtcaggat tgatgccggt gcagcagaaa tcaaagtgac ggattatcga gagaatacaa
     8641 cctactgcgg ttatgtccga tgtattctcg gaaaatgcaa ggtcacggat atgatgtcac
     8701 agctttcgga gattacagca cagataaagg attacaattc aaccattctt cagctgacca
     8761 atcaggtggc agaacttgca acaaaggtaa atgaaatgac cggagatgtg gtttcagtgg
     8821 gcaaatgcgg cgctgatatc aattacgttc tctattctga cggcagactg attctcaaag
     8881 gcacgggggc aatgtatgat tatgtaggtg catatgaaac aagcggaaac aaatctccgt
     8941 tctgcgaaaa tgataacatc acttctgttg tcgtctcaga gggcatcaca acggttggtg
     9001 aatatgcatt tcaatactgc aacaatctga aaatagccgc tcttccgaca acgctcaaaa
     9061 ccatcaagcg aaatagtttc ataccgcaca ttgatgaata tctggttcat cagaatcttt
     9121 acggactgac tgaaattaca attccgtcta aagtgaccga gattgcaaag ttcgcctttt
     9181 caggtacagc tatcaaatcc cttaccattc ctgcatctgt tacaacagtc ggtgaacagg
     9241 cgtttggaga atgtcagaag cttgaaactg ttcgctacag cggtaaggtt atcggtgaca
     9301 gaatgtttgt acgctgtatc aagctgaaga atttcacgat caccaaaagc acaacagaac
     9361 ttatcggtgg ctgtttcaac tactgtgaaa gcctcacgca aatcacctat gagggcagtc
     9421 ttgctgaatg gaacgctgtg aaaaagaaca caaactggga cggtcattca agcagtacag
     9481 tagatagtcc ccttgtcaaa attcagtgcc tggatggata tatggaatat gatgcagata
     9541 cggaaacgtg gaaggaagtg aaggcatgat aaaatttctt gtaaagggac agaacatcga
     9601 aacgctggag catgaggtca ttgcagctga tcagattgct tttgtaaaga tacattttgt
     9661 atttgataac agctggaaac ccctgcataa agtggtgcag ttcacacagg acgagttcac
     9721 ttacaacaga gttctcggat ttgacgagac aagctgtcat ctgccggcgg aacttgcagc
     9781 cggtgctgtg aagatgtctc ttttcggtta tgacgcagaa tcatctgaaa cagtcagggc
     9841 aacaacggtt gtgaaaaccc tgcatatcag accatctggt tttgacggtg aaagcagcaa
     9901 tgtaccgcca acgcctgacc tataccagca gttgcttcaa aagataagtg aaaaaggtgc
     9961 tgacggcaag tctgcatttg agattgctgt agagaatggc tttattggta cagaagctga
    10021 gtggcttgag agcttaaaag gtaaagacgg aaaagatgga gttgacggaa aaaatggtca
    10081 ggacggtaaa gatggaattg atggaaaatc tgcatatgaa atcgccattg caaatggcta
    10141 ttttggcaca gaatctgaat ggcttgagag cttaaaagga aaaaacggta ttgacggaca
    10201 acccggcaaa gatggaattg acgggacaaa tggacaggac ggtaaagatg gcattgacgg
    10261 tacgcccgga actgacggaa aatctgcata cattattgct gtagagcatg gatttaccgg
    10321 aacagaaact gaatggcttg agagcttaaa aggtaaagac ggtaccgacg gacagcccgg
    10381 aaaagatgga gttgatggta aagatggcgt cacgcctgat atgtcggatt atccgaataa
    10441 agcagatttc gaggctttac agcagaaatt acagtctttg caggatagca ctatggacta
    10501 catcatgggg cttacaagca gatgcgactc atttgataca gaaattcagg aaatagacgc
    10561 caatgctcag cagctaagag attctgttca gtttgatttt cagacaaagg aagaagaaat
    10621 cctcgctctt gaaaccagaa ttatcgctct tgaaagccgt tccggcatcg aatacatcac
    10681 ggtcttttct tccggcagtg atgctttgca gaaatatggt gagagcgtct acacctatta
    10741 caacgacggt taccgttcgc ttgcaggctt tgcggaaagc tatccccatt tctgctgtac
    10801 agagaatgac tatgctttgt acttcaatca gtctgatttc agctgggcag gaacagtatt
    10861 tgtgctttgc ctgacgcctg ttgctctcac ttcttctatg aatctgattc tcagttatac
    10921 agtcggtgcg tcacaggacg ctgaatttta tctggtgaag aaaaccgaca agaccggggc
    10981 tgaacttgct cagtatatct atgaggaaat acaggcagga aatgcagtaa ctttacaatt
    11041 caaatggctt tattccgata catatatttc cgtgatgcag tcattggaaa acgtaccgga
    11101 tggagaatat tatcttgcct tcaaaggcac atcggataat tcacatccga tgataaaatc
    11161 aatcaaattt atgggaggat gatgtttatg aaagaatgga tctgtacaat tgcaggcatt
    11221 gtaggcggat ttattgccac gctgtttggc ggatgggact cggctcttgc aacacttgta
    11281 gtgttcatgg gtatcgattt tgcaacaggt ctcgtcactg caattatggg aaaatccaag
    11341 cacagcaaaa gcggagcact caacagcaag gcaggctgga ttggtcttgc gaaaaagttc
    11401 tgtattctgc tgatggttgt ggttggtgtg agaattgata ttctcatcgg cacaaactac
    11461 atacgtgacg cagtctgcat cagcttttgt ctgaacgagc tgctgtctat tgtagaaaac
    11521 accacactga tgggcgtacc ctatccgccg gtattcaaaa aagcaattga tgttctgcaa
    11581 accaaggttg gcagaatgga agaaacaact gacaaggagg aatctgatca tggcaattct
    11641 gaaacctgat aacacatcta ctctgaatgg agtaaaaatc aacgagtatc tgctcaccaa
    11701 acacaatcca aacagtatag caatgcccac cgtttctatg gagggcaaag ttatcggtat
    11761 cactgttcac aataccgatt ggatttcggt agcaagcgga actactcctg cagaacagta
    11821 taccagagca acttacaatg gcaatatgaa agatgtcagg gtgcattatt atgtggacaa
    11881 cacctgtgcg tggcagaatc tacctttaac gcttagtggc tggcacgccg ctgatggaag
    11941 cggcaacggc aaccacagaa cgattgccat tgaatgcatt atgtcatctg cgtataattc
    12001 tacggataag aagtcggaag acaactgtgc gagacttgcc gcagcacttc tgaagaagta
    12061 tggtcttgac attaatcacc tctacactca tacccattgg ctgaatgtac gtgacgggaa
    12121 aagtggtact gtggactatc tcaatactgc aaaaaattct tacaagatgt gtcctgccta
    12181 cattctgcca cattgggcgg aatttaagaa gaaggtcgaa tcttatctga atactggttc
    12241 tacaacttca aatcctacac ctgcaaatca gctttacaga gtaagaaagt cctggtctga
    12301 cgctaagagc cagattggag catattcttc tctggaaaat gcaaagaaag cctgcaaggc
    12361 aggttacagc gtttttgata attcaggcaa agcggtatat accgttgctg aaaagacata
    12421 tacaaaagac acaaaaatta cgctgaacaa tgtcactttg tattcctcca gcacagccaa
    12481 aaacggaaca aagaaatccg gaacgtacta tctctatgat ggacaagtga taaatggcag
    12541 aatgcgtatt acaaattctt ctgcaaactg cggaaaaaca cctaccggtt cttatgtgac
    12601 cggttgggtg aacaaggctg acatctgata acgatcccca cgagatatga ttttttcata
    12661 tttcgtgggg attttttgtt tttcggtact caaaagccat atttccgtcc tatgtaaagt
    12721 aggaggtggt ttcaatgaca gatgcacaaa aatctactgt actcacattg cgttcaaagg
    12781 gaatgtcgtt ttctaagatt gcagaaactg taggattgtc tgtcaacacg ataaaatcct
    12841 tttgcagcag acataaaggg cagttctgcc tttgctgcgg cgagccaatc acgcagccgc
    12901 caagagtacg tcagaaaaag ttctgttcgg ataaatgcag aatgaaatgg tggaatgctc
    12961 atataaaaga tgttaacagg aaagctatgt atgattttat ctgctcaaat tgtggtaagc
    13021 cttttcaggc atacggcaat aatcacagaa aatactgctg ccacagatgt tatattcttg
    13081 cacgattcgg aggtgaaaaa gatggatatt cagaaggaag ccatgtacca ggtgacgatg
    13141 ggcattgtaa agaagatgtt tcatgcaaat ctgatttcgg aggatgaata tcgtcagatt
    13201 gatacaatgt tcagggagaa atacgagccg aaaatcggca cattattcgt tgacttagag
    13261 cctgaacagc ggtaatatgt gtagtgaaag gagagctgtt atgcgtaaaa tcatcaaaat
    13321 agaacctacg gagcccgtat tgccaaggcg aaagcgagtg gctgcttatg ctcgtgtatc
    13381 aatggagtgt gaacgcctta tgcattcaat gtcagcacaa ataagctact atagcgaact
    13441 gatacaaaag aatcccgaat gggagtatgc aggagtttat gctgataatt ttatatcagg
    13501 aacagagacc aagaaacggc aggagtttca aagaatgatt tctgac
//
LOCUS       DAWBGR010000054        13503 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1429_length_13503_cov_4.655265, whole genome shotgun sequence.
ACCESSION   DAWBGR010000054 DAWBGR010000000
VERSION     DAWBGR010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13503)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13503)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13503
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1429_length_13503_cov_4.655265"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3200 a         3671 c         3535 g         3097 t
ORIGIN      
        1 ttgcaagcag atggcgtgca aagtcgccag acgctctttg tgcggtattg ccttacagaa
       61 aaagaacaga aaaaccggct gtcagataac tgacagccgg ttttttagaa tgcattatca
      121 aattaataag taaagccttg tgcagtgctg cccttacttc ttttccgcca ttcccaaagc
      181 aattgccttg aggtttgcct caatgaggtg agcacgtttc ggcggaatgc acttttccat
      241 tgcctttttc acggtgtcca tatcaaacag gttggtttct gccagcattc tgcccagcag
      301 caccatgttt gccgcaccgt tcagtcctgc attggtggca atgtccgttg ccggcagaga
      361 gattgccgtg atgtcggttc tctcgcaggt ttcctcaaca atagagttat cgaacagcac
      421 cagaccgtcc ggcttgactg catcgataaa cttcttatag gacgggccat tcatcgcaat
      481 gagatagtcc ggcgttgtca cgaccggaga accgatcggt tcatcggaaa tacaaacgga
      541 acagttagca gtaccgccac gcatttccgg accgtaggac ggcagccagc tgagttcctt
      601 gccggcgatc agtccgcagt atgccagaat ctttccggca aacagaatgc cctgtccgcc
      661 gaaaccagct aaaatgattt cagttgtcat gcctttgcac cctcctctgc ggtaacgtcc
      721 ttatatacgc ccagcgggta gtaaggaatc atcttgtcct tgacgaactg catggactct
      781 gtgggcgaca tgccccagtt ggtgggacat gtggacagca cttcaatgat cgacagacca
      841 cgctttgcct tttcattttc gaacgccttg cgaattgcct tcttggcttc gcggatatgg
      901 ggaacgctgt cgatcgccac acgctgtgcc aatgcagtac cgctgagcgt tgccatcatt
      961 tcacagatac ggatcggata gccggcagtt tcaacgtttc tgccgaacgg tgtcgtctgt
     1021 gtcacctgtc ccggcagcgt ggtcggagcc atctgaccgc cagtcatgcc gtaggtggtg
     1081 ttattgatga agatcaccac gatattttcg ccgcgggtac caacatgaac ggtttctgcg
     1141 gtaccgatcg ccgcaaggtc gccgtcgccc tgataggaaa atacaaactt gtccggattg
     1201 gtacgctttg caccggtagc cactgccgga gctctgccgt gaggggcttc gatcacatcg
     1261 cagccgaaat agttatagga cattaccgaa cagcctaccg gaacaacgcc gatggtgtcg
     1321 ccctcgatgc ccatttcgtc aatgacttcg cagaccagac ggtgtacgat accgtgaccg
     1381 cagcccggac agaagtgtgt ggaaacgtcc agcagggact tcggatgatc aaatacaaca
     1441 gccattattc tgcacctcct gccagttttt cgatctccgc cagtacttct gccggagtgg
     1501 ggatcacgcc gccggttctg ccgaagaacg ataccggcag acggcactct gttgccagac
     1561 gaacgtcatc gatcatctga cccatagaca tttccacgtc cagaatatga gaagcatgct
     1621 tggttgcttc gcacagttct tttttcggga acggccacag ggtcttggga cggcacagac
     1681 ctgccttgat gcccttgcgg cgggcagcag tgacagcgga cttggataca cgtgcagatg
     1741 caccataagc ggtaacgacg atctccgcat catcacagca gtacagttct gcatcggttt
     1801 cggtttcttc aataatggcg taacgcttga accgttcctg tacctttgcc tccagcagcg
     1861 gtgcgtccag atacagagag ttgatgatgt ggtgttcccg cttgttgccg tgaccgtttg
     1921 cagcccagtc ggacttgtcc ggcagcgtgg tgcggggttc cgggaatgtt accggctcca
     1981 tcatctgtcc cagcagaccg tctgccagaa tcattgccgg catgcggtac tggtcagcct
     2041 tatcgaatgc cagcgtcacg gtgtccacca tttcctgtac ggaacatggt gcatatacca
     2101 gaatatgaaa atcaccgtgt cccatggcgt gggtcgcctg attatagtca gactgtgccg
     2161 gctggatcga gcccagaccc ggaccgccac gctgtacgtt gataatcagc cccggcaggt
     2221 cggaaccagc catgtaagag attccctctg ccttcagcga aacgccgggc gaggaggacg
     2281 atgtcatggc acgcacgccg gtagaggcgg cacccagcac catgttgatc gctgcaattt
     2341 ccgactctgc ctgcaaaaag acaccgccgg atttttccat acgctttgcc atatacgcag
     2401 acagttcggt ctgcggtgtg atcggatagc caaagaagca cttacagccg gcacgaattg
     2461 ccgcttctgc caatgcctcg ttgcccttca tcagacttct ttccattggt tatgaacctt
     2521 ctttcacttt tgaatggtaa ttgcacaatc cggacagatc attgcacaca tggcacaact
     2581 gatacatgcg tcctgatcgg tgcattctgc ggtaaaatat cctttttcat tccggtgttc
     2641 cttttgcagg gcaacgatct gcttgggaca gaccgttacg caaagaccac accctttaca
     2701 gcgttcaaac cgaacctgca ttttctccat attcctcact cctttctgcg tacatttatt
     2761 ctacaagaca cttgaggcaa cgccgcacag gacacgccct tcgggtgcct tttcagagaa
     2821 gcttactttg taaaatctta gggcaatacc gcacaaagag cgtctggcgg ctttgcacgt
     2881 aatctgcttg cagattgctc cttattcctc ccacaccggc ttgacaaaca cctctaccgg
     2941 cagaatgtcc ggtacagaaa cggtcaggtc cttccggcag gtggtgcaga ccagcggcag
     3001 cttcgcctgt gcggcgattt cttccgcata aggcactgcc cgcagaatgg tttccgccgt
     3061 ggtttccttc cccagattgg agttgttcac aattccggtg tgacgcatgc gggaaacagc
     3121 ttcgatctcg tacatcagtt ccagtgtttc ctgcggttct tctgtgagat agcgatagcg
     3181 gttcaccaca taccacatgt ccagctgatc gggaacatag gcggaaaagc cctcggcata
     3241 ccgtcccagt ccaatggcac cggcatcgtc cccgcctacg tcgatgataa gatagccgtc
     3301 ctcatatgcc atgcgttcca tatcaaaaga aatggcagga atgtccagac tggtgttggc
     3361 atacatggaa gccgccagcg tgacaccccg ttcctcaaac agtttcgcaa aatccgccgt
     3421 gcggaaatag ggattgacaa tgtcaaaatc gacaatggtc accttttcgc cggcatctgc
     3481 cagccgcagg gcaaggttga ctgccagatt ggtttttccg gaaccatagt gtcccgtaat
     3541 gatcgtgatc tttttcatag aagttccttt cccagcaaga gcatgtttct ttttattata
     3601 gcacattcta tacaaaaatg caactgaaaa tgttggggca ataccgcaca aagagcgtct
     3661 ggtgactttg cacggtattg ccttggattt tttacagaaa tacagcccgc tgccccctgc
     3721 tttctccgat aaaaaatacc gtactccctg cacggaatac ggtatctgca tgcagcaaca
     3781 aaaagcggga ataacagcgg ctcttatacc cgaatcagtc tgccctcctg caagtccagt
     3841 tctgccagct gtttcatgac ataatgcatc ttctttcctg tgacattgcg gggcagttag
     3901 ctgacaaacc ggtaataatg cgggcgttta taattcgcca gcagcgggct ttccttgcag
     3961 taacgttcca gttcctgtgc tgtcagcgac gggtctgcag cgatcacata ggcggtgacg
     4021 atctcgccac gcctgctgtc cggtgctgcc gtaacaatgc agtccttgac tgcggggtgc
     4081 aggttcagca cttcttccac ttccaccgga taaatgtttt ctccgccgga aatgatcata
     4141 tcgtcttttc gtcctacaat gcggaaataa tggttctcgt cccatgtgcc cagatcattg
     4201 gtatagaaaa agccgtcgtg gaaccgccgg cgggtttccg cagggttgcc gaaatagaac
     4261 gccgccgact tggcgttaga gcgaatgatg acttctccca cctcgctgcc gtctgtcgct
     4321 gcaaggtcat ccggctctgc cacacgatcc tcatatacct ttatcacacg cacatcatca
     4381 tcaacgcagg aacgccctgc actgcctgcc atttccggca ggtcgtccgg tcggaggaac
     4441 gtgttccaga acgtttctgt cgtaccatag ccgttgaaaa tccgcttggt cagaatcttc
     4501 tgatagcgga tacagtctgc ccgttccagc gggctgccca tggtgacgat gccacgcagg
     4561 ctgctcagat cacggggctg tttctgctgt gcttctgcca gcagttccag cacgttggga
     4621 acgccgatga gaaatgtcag cccgtactgc tgtaccatgt ccagtgtctt ttccggctgg
     4681 aatttccgct ggataatcac agtaccgccg gcgtacagcg tcggtgtaat gccgccggag
     4741 tgcagcccgc cgcggtgaaa ccacggtgta gtgttcatgg aacggtcggt aaacgaaaac
     4801 gggaaatgga tcatcacatc atgagcggaa agcacctcgt tgatgctggt caggcatacg
     4861 cttttctgtc tgccggtggt accggaggtg aacagccgca gggtttcatc atagacagac
     4921 agttcccatg gcagctccgg ttcagttgcg ggacagtctg cggtgaagcc agcgtaggca
     4981 tgggctgccc tggcatctgt ggtcagcaga atctgcgggg cgtgtgccgt ccgccgcagt
     5041 gccatgtcca cttccgcctg tgtttccggc tccagcagga acaccttggg acggctgttg
     5101 tccatacagt ctgtaatttc cttggcagac agacggaaat tcaccggaca gaataccatg
     5161 ccggtcttat ggcaagccag ataggcgaac acgaattccg gacagttgtg cagctgtacc
     5221 atgacaacat cgcccttttg cagtccggca cggagcaggg cgtgcgaaaa ccggtttgcc
     5281 tctgcattca gctgtgtgta cgtccagacg gttccgctct ccggttctat cagggcttcc
     5341 cgatcaccga accgtgccac attccgcaga aaaccgttcc gccatgtgta gccgttttca
     5401 aactgctgcc ggaacgcctc catcgcacgc tgcttttcgt ccagaatgcg tttctgccgc
     5461 acctgctgca aaaattgttc cagatgtgcc gtggctcttt ccagagcctc tgctccggca
     5521 gggacagctt cagcagccag aatcatttgc tccatttccc gcagtgctgc catttcgtgc
     5581 tgtttgacac tgcttttgcg ggaggtatgg ctgccggtgc gaaaaatggt ttcggtatat
     5641 tctttgcggt acatggcgaa atcctccttc gatcagaatg taaaatatgc ggtgccgtaa
     5701 ctcaaaccgc cgccgaatcc ggacagcatc atggtctgtc cccgttttac gctgccgctg
     5761 cggacacagt cgtccagtgc aatggcaatc gatgccgcag aggtgttgcc gtattcgccg
     5821 atgttcagga agaacttttc catcggttct ttcagctgct tggctgctgc ctgcaagatg
     5881 cgggcattcg cctggtggca gacatagcag tccggatagc cgcccacttt ctccgccaca
     5941 cgctggatac tgccggagag tgcctgcacg gcaaaactgt acacggctct gccctgcatg
     6001 ctgacgaact gctctgccag ttccggcagg ttttcagcaa aggggtttgt atcgtgataa
     6061 tacttcgggc agtacagcac ttctgtgttg ccctctgctc cgcagtcaaa ggcatagcgg
     6121 ctgttttcgt ccagcgtgat taccgctgca ccggcaccgt ccccgaacaa gacgcaggtg
     6181 ttccggtcag caaaattggt aacacggctc aacacttcgc ttgccaccac cagagcgtat
     6241 ttcttttccg gcatgctgca cagcagagaa cgggcggtgt gtactgcgta gaggaatccg
     6301 gtgcaggctg cacagatgtc aaaggcaaac accgtttccg gcagtcccag ttccttttgc
     6361 agcatgcacg cctgcgacgg aacgatattg tccggcgtaa tggtggcaca gatgactacg
     6421 ccgatttctt ctttagagat gccagccatt tccagtgcct tttctgcggc ttccatgccc
     6481 agagaaagat tcgtttcctt gtccgcaaaa tgccggttgg aaatgccggt gcgggtgcgg
     6541 atccactcat cgttggtttc tacgatcgtt gccatgtcat cgttggaaat cttccgttcc
     6601 ggcacgctgc tgccggtgcc gcagatgcga atgccaatgt cctgtgtgat tttcatatag
     6661 ggttcctcgc tttcatgttg ttgcagtact tagtataccc tttttacgga atagaatcca
     6721 cctattccca cagaaaccgg ctctaccgtt ttgagaattt ccgaatgctg gtgtatattc
     6781 acacaaaaaa ggcatggtac aggtgtacca tgcctttttt cgttatattt caaatataat
     6841 atttgttaaa aacagcggta ttgtctttac tttttccgcg gaaacggata gggaatcagc
     6901 cactggtgct ttgccgcaca ccgccagaaa tagcagagga ctaccggcag ttccagcaca
     6961 ccagccaatg caaacaccac cgccgcaccg gaaatggtgt gaaacgtcac aaagacagag
     7021 agagcccctg cccagatgat gccggttatg gcaatgaaga tccatgcccg ttcccaccac
     7081 aggctggcaa aaatcaggaa caccacaaac gaaccggcaa gagcgtagca cagcactctg
     7141 ccgtcccaga gatctgccac gctcggtgcg aggtactgga acagacagat cagcagaaat
     7201 gccaccagcc aaacgccggc tacggacagt agagtgatga aaaagtgctt ccagcggaat
     7261 ctaccgtaag tagaatctac cgattttgcg tcactttccg gctctaccga caattccggc
     7321 tggggcttcg gcggatagat cagttcgtcc acagaaacgc cgaaaacttc cgccagctgc
     7381 accagtgtcg gcacatccgg aatgccgtcg ccccgttccc atttcgaaat gctcttgtca
     7441 gaatagttga tcttctccga cagatcaatt tgtgtcatgc ccagctgctt ccgatatttt
     7501 gcaatgtttc cggcaatggt tttccgcata ttttcctctg tcattccaaa ggcccctttc
     7561 tgtaaaattc caaagtgctc ttatccatgc atcattgcag atgtaaaggc aacaacgcac
     7621 cttacacaca agctttgtgc ggtgttgcct aaaatcattc gtagaatcaa aagtattata
     7681 gcataagaaa aagttatttt ggtgaaattc ttatgaatct tcataccgga aaaagtctct
     7741 tgacaaacgg cagaaatcat gttatactga tagcagcttc agggtactgt gcaattcaga
     7801 accggcagtg aacggcgata tgccgtagtc tgcgagccag tgtggaactg gttgattcgg
     7861 tgagattccg aaaccgacgg taaagtccgg atgagagaag ccacgcagca aatacgatgt
     7921 gtttccccca taaccatgaa tccatacagc tcccgcagaa aagcggctgt ctgatttcgc
     7981 cctgttgtgc ttctggcaaa acagggcgtt gttatttttc ggaggtgttt tcatgacaga
     8041 gcattctttt tacatggagc gtgccataca gcttgccaag cgtggcaccg gctttaccaa
     8101 tcccaatccg ctggtaggtg ctgtgatcgt caaagatcac cgcatcatcg gcgagggctg
     8161 gcatgcccgc tatggtgacc tgcacgcaga acgaaacgcc ctgaagaact gcacggagga
     8221 tcctgccggt gcgaccatgt atgtcacact ggagccatgc tgtcaccacg gcaagcagcc
     8281 gccctgcacc gaagcactgg tggaaagcgg cattcggcgg gtgtacatcg gttcccgcga
     8341 tttcaatccg ctggtattcg gcaagggtgt gcagtatctg gaagaacacg gcattgaagt
     8401 gattccggat ttcctgcggg aagcctgtga taaactgaac ccgatcttct tccggtatat
     8461 tggcaccaaa atgccctata ccattttaaa atatgccatg actgctgacg gcaagatcgc
     8521 ctgtgccggc ggggcttcca aatgggtaac tggcgaagct gcccgtgccc atgttcaaca
     8581 gacccgaaag cgggtggctg cgatctgtgt cggcatcggc acagtactgg cagacgatcc
     8641 tctgctcacc tgccgctgtg agaatccttc ccagccggta cgggtggtac tggatacccg
     8701 cctgcgaatc cccatggaga gccggctggt gcagactgcc aaggaagtgc cggtcattgt
     8761 cttttgctat gctccggaca aggacaaaaa ggcggctctg gaagcggcag gcgttaccgt
     8821 actggaggca aagttgctgc ccaaggaaga actggacgag ttgttcatgc ggctcggcga
     8881 atttcaactc tccaaggagg aactgaacgg cagacacatc tccctgcgaa actgtctgga
     8941 acagctggga cagcgtgggc tggacagcgt gctgattgag ggcggtgcga ccattcacgc
     9001 cgcagcattg caggaaaatt gctgtgatct ggtgcaggtt tacgtggcac cgaagctgtt
     9061 cggcggcgac ggtctgtccc ccattggcgg catgggcatt accacaccgg acaaggctgt
     9121 cctgctgtcc gaacccacca ttacccgtct gggacaggat ctgcttctgg aatataccag
     9181 aaaggagcaa ttttaagatg tttaccggaa tcgtagaaga aatggggact gtgcggcggc
     9241 tgaaccagtc ccccaaccgg tgcgaactgg aactgtccgc cacaaaggtg ctggagggga
     9301 cgcagatcgg cgacagcatt gccgtgaacg gcgtttgcct gacggtgatt cgcatggaca
     9361 aagaccactt cacagcagat gtgatgccag aaaccctgcg gcggagcaat ctggggcagc
     9421 tgaaaaccgg ctcgaaagtg aatctggaac gtgccatggc ggcggacggc agattcggcg
     9481 ggcacattgt cgccggacac attgacggca cgggaacaat ccgttccatg cagccggagg
     9541 gcaacgcaat gctagtgacg atcagtgcca caccggaact gctccggtat gtggtggaaa
     9601 aaggctccat tgccattgac ggcatcagcc tgacagtggc aaaagtttcc catacggatt
     9661 tcactgtgag cctgatccca cacaccggtg aggaaaccac cctgctgaag catcgctccg
     9721 gtgagatcgt caatctggaa accgacatca tcggcaagta tgtggaaaag ctgatgcaac
     9781 cgaaaagcgg cggtgtcact atggaactgc tgcaacagaa cggatttttt taaaggagat
     9841 gcatatgcgg catatcattt gttttcacaa tccagaagaa gaaaacggct atctcagcaa
     9901 ctggtatccc tcgccgttcg agaaagacgg catttccttt gcccacatgg aacagtacat
     9961 gatgtaccag aaagccctgc aatttcagga taccgaaatt gcacagcaga ttttgcaaac
    10021 cgacaacccc ggcaaggtca aagcactggg gcggagcgtc cggaactatg tggactcccg
    10081 gtggagtgca gtccggcagc agatcgtctg cgaggggctg ctggaaaaat tccggcaaaa
    10141 ccagtcgctt ctgcaagaac tgctggcaac tgcaccgcat ctgctggcag aatgtgcggt
    10201 acatgacagg atctggggca tcggactgtc catgacagac agcgaccgtt tttctccgga
    10261 aaaatggcgt ggacaaaatc tgttgggtta tacactgatg caggtacggg acgaactatc
    10321 caaataagga ggtcatttct atggcaatta catataatac cattgaagaa gctttgcagg
    10381 atttgcggaa cggcaagtgc attctggtaa cagacgatcc ggatcgggaa aacgagggcg
    10441 acctcatctg tgccggacag tttgccacca cggaaaacgt caacctcatg gcaagtgccg
    10501 caaaggggct gatctgcatg cccatgaacc atgacaacat tgtccggctg ggactgacgc
    10561 aaatggtttc tcacaacacc gacaaccatg aaaccgcgtt caccgtttcc attgaccata
    10621 tcgacaccac caccggtatt tccgcagtgg agcgtggtct gacagcccgc atggcagccg
    10681 atccggaagc acaccccggc gacttccgcc gtccgggaca catgttcccg ctggaggcca
    10741 agcccggcgg cgttctggaa cggaacgggc acaccgaagc aactgtagac ctgctccggc
    10801 tcgccggact gacacaggta ggactttgct gtgagatcat ggacgatgac ggcaccatga
    10861 tgcgtggcga aaagctggca gactttgcaa aacagcacgg gctgtccttt atcaccattc
    10921 gggaactcca gcactaccgc cggaatcatg acaacttcgt ggaagaagtg gcacgggcag
    10981 aactgcccac agtccacgga catttccaga tgttcggttt ccgcaacacc atcaacgggg
    11041 acgagcatgt ggctctggtc atgggcgatg tggcagacgg caagccggta ttgtgtcggg
    11101 tacactctga gtgtctgact ggtgatgtgt tcggctcctg ccggtgcgac tgcggcgaac
    11161 agctgaaccg tgcactggac atgatccacg cagagggacg aggcgttctg gtctatctgc
    11221 ggcaggaagg cagaggcatt ggtctgctga acaagattcg ggcgtacaag ctacaggaag
    11281 acggactgga taccgtagag gcaaatatca aactgggatt tgcaccggac ctgcgggatt
    11341 acagcgtggg tgcacagatc ctgaaacatc tgggggtaac ttctctgcgg ctgatgacca
    11401 acaatccgga aaagatcgca gagctgggcg aatacgggct gactgttgcc gagcgtgttc
    11461 ccatcgacat tccggaaaag ccggagaatc tgcggtatct caagaccaag cagttcaaaa
    11521 tgggtcatta tctgcacttt taaggggatt tgtctatgaa aatcatttat agtagtatgc
    11581 ttcctctgct ggcagctgtc tgtgtatgga caagcggctg cggctcgctg gagtccgctg
    11641 tggaatcttc cacaacaacc gcgactactg ctacagaaac gacaacagaa actactgttg
    11701 cggaaaccac tacaacatct gcaacagaaa ctactacagc atccgcaaca gaaattgcta
    11761 cagaaattgc tgcaatacca acaacagaag ctacggacga tgctgcggca atagaagtgt
    11821 tcgcctgcac tgccggcacg tggtatatgg acggagatcc cgatacggct tctctggtca
    11881 tggacggtga aggcggcgtg gctgcctact acgccaccgg caatctggaa tacagcggtt
    11941 atctggaatg caccgatgcg acatcaggaa catacgccat ttatcaggac agcggcgaat
    12001 atgttacaga ttttaccttt gatgatgcgg acacatttca catcaacagc aactggggac
    12061 ctgtgacatt ccgccgtact tagggcaata ccgcatcagc gtttccttag acgttgtcca
    12121 cagaaaggaa gtgattcccc tgcacctatt attctgtatc aaccgcaatt tttgctggca
    12181 gctggtacaa tgcctgcgtt ctgtggtacg atttccctgt tcggagggct acaccgtcca
    12241 tgtgctgcac tccgatctga cagcggacga tctgcaacag atacagcaga aggttccgga
    12301 agcggaaatt ctgggggtgt ccatagacga aacctttttt caggactttc cggaaactga
    12361 ccgctatccc aggcaaatct actaccgcat ctttgcggct cagtttttac cgacagaatt
    12421 agaccgtgtg ctgtatctgg acaccgacac gatcgcaatt cagccgctgg acgacctgta
    12481 ccacaaggca tttgacggtg caagctatat tgcctgcacc cacgtgcgtg ctctgttcaa
    12541 caagatgaac tgcctgcggc tgggcattgc ggaagatgtg ccctacatca acaccggtgt
    12601 catggtgctg aacctgaaac aacttcggca gcagcagacc accgaggacg tgctggcatt
    12661 tgtcagcgac aagaaaaacc gcctgctcct gccggatcag gacatcatca ctgctctgta
    12721 cggcaagcag attcagctgg cggacagcta tcgctacaat ctgagcgatc gtattctggc
    12781 aatgtacaac gccagacacc cgaacgagat ccgcagtatc gactgggtca gacagaacac
    12841 cagcgtcatt cactactgcg gcaaaaataa accatggaag tccggataca tcgggaaact
    12901 gggtatcttt tatcaggaac ttctcgaaac tgaaaacaac aacggaggta caaaaacatg
    12961 aaaacactgg aaggcatgta tgacggcaaa gggctgaaaa tcgcgatcgt tgcatcacgt
    13021 ttcaacgaat tcatcaccac caagctggtg ggcggtgcag aggactgtct gctgcggcac
    13081 ggtaccgacg gcgacgacat cacactggca tgggtaccgg gggcatttga gatcccgctg
    13141 gctgccaaga agctggcatt ctccggcaag tatgatgcga tcatctgcgt cggtgcagtg
    13201 atccgcggtt ccaccagcca ctacgactat gtttgtgcag aagtttccaa gggcattgca
    13261 caagtaggac tggagtccgg tctgccggta ctgttcggcg tgctgactac cgacaccatt
    13321 gaacaggcaa tcgagcgtgc cggcaccaag gcaggcaaca agggttatga ctgtgccatg
    13381 tccgcactgg aaatggtcag cctgttcaag aacgcctgaa aacgagaaac gaggcacttt
    13441 ccacattgtt gaggcaatac cgcacaaaga ttgtgcgaca gatgcgtcgt caaatttttc
    13501 gtc
//
LOCUS       DAWBGR010000055        13467 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1437_length_13467_cov_5.123322, whole genome shotgun sequence.
ACCESSION   DAWBGR010000055 DAWBGR010000000
VERSION     DAWBGR010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13467)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13467)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13467
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1437_length_13467_cov_5.123322"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2909 a         3889 c         3594 g         3075 t
ORIGIN      
        1 gccagacgct ctttgtgcgg tattgcccaa gaggaacaca agggaagaag tattatatca
       61 tggcaaaagt atccaaaaag cagttgggac ggcggaggag aaccgcattg cttctgcttc
      121 tggcactgat cgcggcagca gtctacctgc tctttttccg gtcagacagc agcagaaacg
      181 ttcctacaaa agaaaccacc gccgtactgc aaagcacggc aatgacagaa acgactacta
      241 ccgaggagat cggcgtgctg tatcagggca ccattccggt acagacagaa ctcaccgttc
      301 ccaccgagcc ggcagtgctg acggcttcgc aggtggaact ggacgcacag ccggtcctgc
      361 aaaatccgga actgcctacc ggctgcgaag tgaccacact gacggcagca ctgaactatc
      421 tgggctaccc cgtggacaag ctcaccatgg cagaccagta tctcacccgt gctgaaccct
      481 atcaggcaac attcggggaa gcttttatcg gctcgcccca cgatgccaac gcgtggggct
      541 gttatgcacc ggtaatcgtg gaaaccgcac agaaatacct tgacgaacag gacggcggcg
      601 aggtggcaca gaatctcaca ggctgttccc tgaagacgct gctgtgggag gtagccaacg
      661 gcaatccggt catcacgtgg gtgacgatca acctcaccag ccgcgtggaa gaacgctact
      721 actggacaac gcccaaggga gaagatgcgg tattcctcat caacgagcac tgcgtcctgc
      781 tctgcggcta cgacctgaac gccaatacgg ttaccgtctg cgacccgctg gagggcaaaa
      841 tccagtacga catggacaag ttcgagaacc gctaccagct ggtctatcag caggcagtgg
      901 tgctccggaa gccggagagc ctgacgggga cagaaacgga aacgacggaa atgtttgtgc
      961 aataagaacc tgtcttcaca agcgtataca cgtgtctttt cggcaaattt tgttgcatac
     1021 tgcgtgaaaa atacttgccg ggcgacagcc cgcctgcgta tttttgcctt gtctgcaaca
     1081 aaattatgct cgaaaatcca catatatttc ttgtgaagac aggttctaaa aaccaatctc
     1141 gcaacaggag gtatgcccta tggcgtatat gcccgcactc tcttatgaag ccattgcaga
     1201 cggcttgacg gtctgcacca gttcgctgca ccggttcggc acagatgcgg tgctgctgac
     1261 ggaattttcc ggttatcggc aaaaagaccg cgtctgcgac ttcggcaccg gctgcggcat
     1321 tatccccatg ctgatgcagc ggcaggctcc cccgcaggag atctacggcg tggacattca
     1381 gccggaggcg atccggcaga tgtgtctggg cattgccgaa agcacagccg tacagtctgt
     1441 cctgcaccct gtctgtgccg atctgaagaa tctgtgggaa gatgctccgg cgggacagct
     1501 ggatctggtc acctgcaatc cgccgtacaa ggcatatcag gcaggcattg aaagccagct
     1561 gacggcacag aaaattgcac ggcacgagat tttgtgcaac atctatgaca tctgccgttc
     1621 ggcaaagcgg ctgctgaaat tcggcgggcg gctgtgcatc tgcaaccgtc cggaacggct
     1681 ggcagactgc atcgatgcca tgcggcaaaa cggcattgaa ccaaagcggc tgcaatttgt
     1741 ggcaaaggct gccgatgccc agccgtggct gttcctgctg gagggcagaa gcggcggaaa
     1801 gccgtttctg aatgtgctgc ctctgcgaat cgtcggggac ggcagcggcg tgacaacacg
     1861 gctcgaaaag acaatgccgg tatgatctgc actgcggtaa cggttaaggc aacactgcat
     1921 aaagcccgcc tgacggcttt gtacgcaatc tgccgaaaaa tctgactacg catcttacgc
     1981 acaagcttta ttcagtgttt ccctagaaat cgtatcagaa cacatagaaa ttgtattaga
     2041 aacccgctgt ttatttttgc tataataagg gaaacactgc acgcaatctg cgtgcagtgt
     2101 ttcctgaaat atgaaactgg ctctaaggca attacttttt tgacatcaca ggaggaacta
     2161 tccccatgag aaaacacaat ccgatctaca agcgggcatt gatgctggcg gtgccgatga
     2221 tgatccagaa cggcatcacc aacatggtca gtctggtgga caacatcatg gtcggcagtc
     2281 agggcacgga agcgatgact gccgtttcca ttgccggtca gctgatcttc gtattcaacc
     2341 tcgccatttt cggtgccctt gccggtcccg gcatctacgg ggcacaatat tacggacagc
     2401 ggaacatgca cggttttcag agcacgttcc gcatcaagct ctggctcagt ctgacatgtc
     2461 ttatcggcgg actgctggtc tttctgctga gggacgagtc tttgatcggg ctatacctcc
     2521 acggagaaag tgccaccgtg gactccgccc tcaccatgac ctatgccaag cagtacctgc
     2581 acatcatgct gctggggctg ccgttctttg tggtgacaca ggtctatgcc accagcctgc
     2641 gggaaaccgg cagcagcgtc aagcccatgg tgggcggcgt ggcttcagta gtcgtggaca
     2701 tcgtactgaa ctacctgctc atttacggca actgcggcat gcccaagctg ggcgttcgcg
     2761 gagcggccat tgccaccgtg ctggcacgtg tcatcgaaat gagcatcgtc atcatctggg
     2821 cacacctctg ccggaacaag cacgtgttcc tgaaaggcat ctaccgcaca ctccgcgttc
     2881 ccaaggaact ggcgggaaag gtactccgca agggcatgcc cattttctgc aacgaactgc
     2941 tctggtctgc cggtctggct gccctgacac agtgctactc tacccgcggg ctggaagtgg
     3001 ttgccggact gaacatctcc aacgccatct gcaacctgct gaacgtggta ttcgtggcac
     3061 tgggttctgc ggtggggatt ctcatcgggc agacgctggg ggcttcggaa tacgagaaag
     3121 ccaagaagga ttcgttcagc ctgatgtggt tcaccggtgc agtctgtctg ctgctggcgg
     3181 cgatcctgat ctccgtttcc ggcgtgttcc ccaaggcata tgacactacc gcagaggtac
     3241 ggaactacgg ccgctggttt atcaccatta ccgcggtgtt cttcccgata cagggcttcc
     3301 tgaacgccct gtacttcacc ctgcgttccg gcggaaaaac gatgattacc tttttgtttg
     3361 acagcgtctt tacatgggtg atcccgattc ccgctgccct gctgatgtgt cacttcacct
     3421 cgctgccgat cctgggcatt tatgccattg tacaggcggc ggatattctc aaggtggtga
     3481 tcgggtatat cctgattcaa aagggtgtat ggatcagcaa tctggtagag gagatgcagt
     3541 aaccggcatt ttcaggaaac aacaccccaa gaccgtcttt tccaaaggcg gtcttttttc
     3601 tgttctttct gcatagaatg gcaaaaattg aggaagcagc accgtgtttc ccgcacccca
     3661 cacagaaagg acgaattgcc atatgtgcac atcatctgcc ggactgccgc cggaattatc
     3721 tcaaacggaa ctccctgtcg tcgcccttgt aggcatgccc aatgtgggaa aatccaccgt
     3781 gttcaacgcc ctcaccggca tgcaccagca caccggaaac tgggcgggaa aaaccgttgc
     3841 cagtgccgtg gggacagtca cctggaacgg ggaatcgctc ctgctggcag atctccccgg
     3901 cacatactcg ctccgtgccc gcagtgccga ggagatcgtg gcacgggatt ttctctgctt
     3961 tgcagagccg gatgccgcgg tagtggtctg tgatgccacc tgtctggaac gagggctgct
     4021 gctggtcatg caggtgctgg aaacctgccc ccgcaccctg ctctgtgtca acctgctgga
     4081 cgaagccgaa aagcggcaga tcgccattga cctgccgcaa ctggaacagc tgctgggcat
     4141 tcctgtggtg ggcatggctg cccgcagcgg caccggactg ccggaattcc aacagcggct
     4201 gttcggcctc ctgcaccagc cggcaccgca gccgctgccc tgtcccgttc cggaacagct
     4261 ggaacagcca ttggaacaga tccagaacga actgccggaa aacctctgcg gactgcagcg
     4321 ggaatggact gcccgacggc tgttggaacc ggagccggaa ctgcacgaaa aaattgccgc
     4381 acacgggaat atttccttgg aaatccccgc actgtccgat gcactggaac agacgtggaa
     4441 gcagtactcc cccgaagtct gtgccgatgc catgatcgcc gccattgtcc gacacagcga
     4501 agctctcgcc gcccagactg tacaccagcc ggaacagacc gatgccgccg accgccgcct
     4561 tgaccggatt ctggcgggaa agtggggcat tccggtgatg ctgctgttgc tggggctggt
     4621 gctctggctc accattgtgg gggcaaatta tccctcggca tggctctccg ccggcttttc
     4681 ccatttgcag ggctggctct ctctgctgct ggaaaccata catacgcccc actggctgca
     4741 cggtctgctg atcgacggca tctacaacgt gctgacctgt gtcattgcag tcatgctgcc
     4801 gcctatggcg attttctttc ccctgttcac cctgctggag gattttggct atctgccccg
     4861 tgtggcgttt catctggata aaccatttca gcgtgcctgt gcctgcggca aacaggggct
     4921 gaccatgtgc atgggcttcg gctgcaacgc cgccggcgtg accggctgcc gcattatcga
     4981 ctccccgcgg gaacggctga tcgccattct caccaacaac tttatcccct gcaacggcag
     5041 attccccacc atgatcacct gtatttccct gtttttcgcc gccggcagcg ggctgtgccg
     5101 ttctgtcagt tctgccctgt tgctgttggg gctgatcctg ctgggcgttt ttgtcacatt
     5161 cgccgtgtcc cgtttgcttt cgcataccat tctgcggggt gtgccgtcct cctttacgct
     5221 ggaactgccg ccgtaccgca agccccagtt cggcaaggtg ctggtgcgtt cgctgctgga
     5281 ccggacgatt tttgtactgg gcagagcctg tctggcggcg gcaccggcgg gagccgtgat
     5341 ctggtgcttc accaactgcc atatcagcgg cggctccatt tttgcacact gtgcggcact
     5401 tctagacaca ccggcacaat tcatcggact ggacggcgta attctgctgg ggttcctgct
     5461 gggatttccc gcaaatgaaa ttgtcctgcc cattatctgc atgggctaca ccgccggcaa
     5521 cgccctcgcc gagatcaccg acttgagccg cattcacgag atcctgacga caaacggctg
     5581 gacggctgcc accgccctgt gcatgctgct gtttttgctg tttcattttc cctgttccac
     5641 aactctcctg accattcaca aggaaaccgg cagctggaag tggacactgg cggcgtgggg
     5701 gatccccacc gctgtgggaa tcctgctgtg cggcgtagtg cactggacgg cagtacttct
     5761 ggcttatatt tgtaaaattt tgtaaatcaa atacaactgc atatctccgc ggattccgga
     5821 tacactatag gcaacgcggc ataaagcttg tacggaagat gcgtcggcag atttttcgga
     5881 agattgtcta aaaatttcgc aggcagactt ttgtctggca aggaattttg aggcaatatg
     5941 ccggaaaata tgcaagcaga tttcgtgcaa agccgtcagg cgggctttgt gccgcattgc
     6001 ctattcaacg cggcaaattt ctgccgccgg ctgccggttc ttctgccgca cacggagaac
     6061 catcagagca gtcctgtgtg cggagaaacg gagaacacca tgtccaagca gaatcagagc
     6121 cagaaccgtc agcagcagca gagccagaat cgccagaacc agcaggcaca gcagaagcag
     6181 aaccagtctg cccagaactg ccggaaccag cagggcggcg acgatgacga ctgtggctgc
     6241 ggctgcggca gataacttct gaaatttgat gttataaaac agaaaacagg acagcttttc
     6301 cctgcggaat cgctgtcctg ttttttgtgg catgctactg ccttatgtac ttaaaatttc
     6361 gtcccagctt ttgggaatgc cggctccccg atacgcacag tacagcatca gatagaatgc
     6421 atctgtggca tcgatcttac tgttgccgtc gcagtctgcc gctgccgcct gcgacatgga
     6481 caaaccggaa gcatagccca cggcatcttg tgcctgtgcc agcagggact ggaacacatc
     6541 ggtggcatct acgctgccgt tcccgtctac atcgcccagc aaaaacaaag gctctgcggt
     6601 ttccttggcg tagatatact gagaatactg tatcagccct tcccgttcca gtccggcaaa
     6661 gagattggcg gcggcattgt ccacctctgc ctgcgtggca tcttccgaca gggctgccgc
     6721 ttcggcatag gctgcctgat acagcggatc tgcatcggcg gcaggctcgc cgtaagacat
     6781 ggcggcattc cactgtgccc agttgaccgc agtgtcctgt tcccgcacaa ttactttaat
     6841 tgcagcgttg cccagattgg aatagggctt tttgccgctg ctgttttgcc actgcttcac
     6901 gtcaaccata tcgatccatg tgtcgccgct gcaaataaac gtctgccccg actggatcga
     6961 gcagtggcgt tccagcgttt ccgtggagtt ggactcaaac gaaagccaga gtttttccct
     7021 gctgcccttt tcctttcgca gttccagcac aaccgaaaac cggtcgccct tttgcagcat
     7081 cacattttgt gctgccagtg aaatgttctt atagccgccg tactgcactg tgccctgttt
     7141 gctgcaaagc ttcgtgccgt ctacagggga ctgggcgtta tttttcagcc ggtacaccga
     7201 aatggtatac cacccgccct gagaattgtt cgatgtcatg ggaaacatga cttccgtcag
     7261 tttgccggac ttttccgcag tgaacacatt ggctgctgcg gaaaagttta cattggcata
     7321 ggaaacactg ccatcgtact gatagcgtgc aacaggtgtg ctgtcctgtg tcatctggaa
     7381 ccgtgccgct tcgcagagag aagcttcctc ataggacagc cagaagtagc cgccgccaac
     7441 atcatctgcc ccccagctgt tccgcaccag ccacgccccg tccgacttgg gctgacagct
     7501 tttttggaaa ttctctctgg aatagttatc gtcccagccc accagcagaa ccgcatggtc
     7561 agcatctccg gtgccgtgcc gcttctggta ataggcaatg gagtccccgt tgtcaaaaaa
     7621 tatgcccttg gaatagaacg atgcactgac ggcaccgctt tccatgagcc actgcttgat
     7681 gacagtccgc cccgcggtgt ccacctgctg caagaggtcg gagctctgca tgcggtagta
     7741 ggcgttatac cgctgatact cggaataacc ctgtgaccag tcgtccatgg aggcgaactg
     7801 ttccagctgg gggccaatgc ctgccgccag tccgtagccg gcaatggtgg ggttgccgcc
     7861 gctgatgccc ttgttgtcca tggtgatata gtcgccgctg gtgaggtcgc ctaccagcaa
     7921 atgattggta aagggatacc agcccagtgc cgcttcggag aagttcagtt cgccgctgct
     7981 gtctttccac gattccggaa tgtccagccc ctgtttcatg atgttggact cacaggaacc
     8041 cagtgccgca tacgcccagc aaagacctgt ggagccctga tctttgacag aagtgacata
     8101 actgctgccg tccacgtcca gcagactata ggaagacggc agcgagccgg ctccacgcag
     8161 gctttcttcc gcctcgtcgt ctgtgacctc cggtgtgtcc gtctgtgccg gctcattcgc
     8221 cggcaccagc agcccgtcgt tctgtttcca cgtttcttcc agaacctccg gcatgtccgg
     8281 ctccgccgcc gcaggaagtt gtccccacgg caaacagctg atgacacaca gcagcgaaat
     8341 cacagcacgc cgccagtacg tccatttttt cctcatatat tcctcctgcc attccggcaa
     8401 tgtgtacgaa attttgtata cgatattatc aattatagca cattccgcca gagatgtcaa
     8461 acaaaaacgc cggagcgttt cctttgagat tcgctccggc gttatttttc cgtgcggtat
     8521 tgccttatga ttcctgcacg gcatcgtaca gttcgctttt ccggtagccg gtttctgcgg
     8581 caacggtttt gcatgcctgt gtcagcttct gcccgtccgc cacacagcgg cgtacctgct
     8641 ccacagcttc ttccagcgtg acgctctccg ccttctgggg caccgcaccg gcaacgacca
     8701 gcacgtattc cccgcggggc ggcgtgtttt cataataggc aatggcttgt gcaaaggtgg
     8761 tcagctgcac ttcctcgtga atcttcgtca gttcccgaca gagggacagg cttctgtccc
     8821 cgccgaacac tgccgccata tctgccagtg tccggcaaag cttatgggga gcctcgtaga
     8881 aaatcatggt tcgctgttcg tcccgcagtt gttccagatg tgccgcccgc tggttccggt
     8941 tcacggagag gaacccctca aaggcaaacc gtgaggtatg ctgtccggag atcgccagtg
     9001 ccgcaatggc tgccgacggt ccggggacta cttccatagg cacacccagc tgatgacaaa
     9061 gctgcaccag cggctcacca gggtcggaaa tacagggcat gcctgcatct gtgaccacgc
     9121 cgcaggattc ccctgcgagg atccggttgg caatgctctg tgccactgtc agcccgctgt
     9181 gttcgtggta gctgacaatg ggtgtgtgaa tgtcatagtg ggagagcaac ttgcgggtga
     9241 cacgggtgtc ctccgccgcc agaaagtcca cctcgcccag cagccgcagc tgccgcatag
     9301 tgatgtcctc cagatttccg atgggcgtgc cgataatgta tagtgtacca ctcatgttga
     9361 aactcctttt gccgccagac gctctttgtt cggtattgcc ttagagaaac tgcttcattt
     9421 ccagaatatc gtgcacatgc accgttgccg tcgcacagat ctgctgccat gccgccggat
     9481 tggctgcatc atacaccccc acggtgaaaa atccggcagc cctggcacat tgcagagcgt
     9541 ggagagaatc ctctgccacg gcgatctgcc gtctgcccaa atgcagcttc tgtgccgcca
     9601 gctgaaagat acggggctgg gtcttgggag caccagcctc ctgctcggtg agcagaaatg
     9661 ccagccgttc ccatacatga agccgtttca gtgccgcctc tgccagttcg ccgtaagtcg
     9721 ccgtggcaac gccatagacg atctgctggc tgtccagcca gtccagcagt tccatgacat
     9781 ggggtttcag cggcagcagt tcccgatact cctgtgcaac ggtatctgcc acctgctgtg
     9841 caaactgttc cggcgtgacc ggcaagtgga actcctccac aaaatagcgg gagaattctt
     9901 caatggacat tcgctgtacc gtttcggaca ggtcgtccgg catttctttg ccgtagtagt
     9961 ccatgatctt cttgtccacc tgccgccaca tgctgccgga atccagcagc gtgccgtcca
    10021 gatcgaaaat gacgccctgt agggtgttgt gttccatggt gttgcgattt cctttcgtgg
    10081 gaaaagcaac accgcacaga gccagcctga cggctttgca cggtattgcc tgaggtttgt
    10141 ttcccgatta cagcttttcg aaccgcggct ggttatcgtg tgccgtgccg gtaaagatca
    10201 acttcggcat tgcccagacg ctgccgtcct gctccttgcg gtaaactacc agttcttcct
    10261 gtgtttctgc gtggcggacg gaggtctgga gcagttcgca gacagagccg tccagtacat
    10321 cgcgatattt tgcaccggct tccagaaccg taggctccgg tacggcaact gctgctgctt
    10381 ccggttcagt ctggttgaca atatcgtcct cgaccagaat catcgaggag aacggtgcca
    10441 tgcggatcca tgcaccgccg ttttctacct gcaccggctg accgctgatg acatcgacca
    10501 gcgagggcac gtgggtgccg aagcggaatt cgctggggca gtcctccaga ttcagtgcca
    10561 cgtaaacggt tctgccgtcc tgctccttgg cgaacagcag ctgacggttc cggacttcca
    10621 cagtgtagta agagccggta cgcagtgccg gatatgcgtg gtagattctg cccagcttta
    10681 ccagatgccg gtacaagtcc agattgccgc tgcggttcag gtcgtccaga tccagacaag
    10741 gccgcagacc gtcatcggaa ttgttctcgt gtctgcccat gacaccgtat tcactgccgt
    10801 agtataccga cggaatgccc ggcatgcagt acatcagcgt atagcacagg ggcagatacc
    10861 gctggtcgca cagggtgctt gccagacggt tgacatcgtg gttgtccaca aagttgtaca
    10921 gagccacgcc ctgatagatg ccgccgttgc cgaactggcg gttgatgctg tagttgatct
    10981 cgaaatagtt cttgtcgttg tgggaagaat agatgccctt atagcactcg tagttggtca
    11041 cgctgtccag catttccgga tttgcccaac gcttatagtc gccgtgaatg atctcgccca
    11101 tgagccagaa ttccggattg cgttccttgc agaagttgtg gatccggcgg aagaagtcga
    11161 agtccacaca gtctgcggca tcaaagcgga taccgtcgat atggaactga tccatccaca
    11221 tgcccacggc atcgatgagg taatcgcaca cgtgccagtt ccgcagattc agcttgacca
    11281 gattatagta gccgttccag ccctcgtacc agaagttgtc gccgcagggg ctctgaccgc
    11341 cgaagttcag accggcaaac cagtcacagt actccgaagc ctgtcccttt tcctgtacgt
    11401 ccttgaacgc ccagaagttc cgtcccacgt ggttgaacac gccgtccaga atcacgcgaa
    11461 tgccgttctt gtgcagttcg tcgcagatgt gggcaaacat ttcgttggtg cccagccggc
    11521 ggtcgatcac acggtaatcg gtggtgtcat agccgtgttc cacagattcg aataccggtc
    11581 cgaagtaaac ggcatcaaca cccagttctt tcagatgcgg gatccagtcc agcacctttt
    11641 ccaaccggtt caccggttcg ccgccctcgt tgcacttcgg tgctccgcag aagcccaagg
    11701 gatagatgtg atacatcact gcgttttcgt accaattcat ttttatttcc tcctactcct
    11761 attatctatt acaaacaagg caatttccct gttggagttc tttaggcaat accgcacaat
    11821 ctttatgcgg tattgccttt aaagcctgtt caggctccta tcacttcaca gacccttcca
    11881 gataatacgt tgcttccata tccgccacgt tgactgccag agccagcgga aacatttcca
    11941 gtgctctgcc cacgctgttc ttgtcctcgt tgccggaaaa gcccatgtga tagcgaatgg
    12001 caaaagcttc gtcacgggtc agccgcataa agctttgaat catgtacacg gatttttccc
    12061 cgtgcccata gggcagggtg tcccgcacgg tatagtaggg caccttttcc cacacgccct
    12121 gttcattctt ggcgttgcga tagtccacgg cataaaagcc aaccttgcag atgtcgtgga
    12181 gcagtgccgt cacggcaatg gtttccggtg tatagtccat gccgtacagt tccttggtgc
    12241 ggggacgatt cagatagtct ttcaggcact cgtacacatt cagactgtgt tccaccagcc
    12301 cgccctcata tgccccgtga aatcgggtgc tggagggggc agtaaagaaa tcgctggcat
    12361 cagagtccag ccattccagc agccggtctg ctccccgccg ctggatattt tcctgataaa
    12421 cggcaataaa ttcttctcgt ttcgtcacgg catttcccgc ctttctatac tctgttgtat
    12481 attataccac acattcaaga aattggcaag cgttccgcga aaaatcccat gagaatccat
    12541 ttgcgtttcc cgccaaaatg tgctatacta ctatagtaag tctatactaa ctaaaaagga
    12601 gcaccgctat gacaaattcg aaaatcaaac cggaactgaa cggcgtggca gaaaccatgc
    12661 tgctgacttt ttatgcaaga gccaagtact cgaaatcccc gaagcatgcc ttttatgatg
    12721 ccaaggcggt ggagctggcg gaacggctgg actacgattt ttccaaggct tccagggacg
    12781 ttgccatggg cagcggcacg attgccagaa ccattgtatt tgacgaactg gtgcagacat
    12841 ttttgcagaa gcaccccgat gcaacggtgg taaacattgc ctgtgggctg gacaccagag
    12901 tgtaccgcat ggacaccgga aagtgcacat ggtacaacct cgacctgccg gaaaccattc
    12961 gtgtccgcga ccagatcttt caggaacagg gacgcatctc cacggtgggc aagtccgtac
    13021 tggatcccac gtggcacgag gacatcaccc aacgcggcac catgctgttc gtcattgagg
    13081 ggctgtccat gtacctccag ccggaagaag tggcacagat gctccgcatc attcacgaca
    13141 acttcgacaa cgcctatgtg atgatggaaa ccacctcgcc gttctgggtg cggaagcagt
    13201 ccattgaaaa atcggtacag gacaccggtg cagtctttac atggggtgtg aaccggttct
    13261 ctgagatgca gaaccttgcc caaggattcc acagcgtgaa agacgacaat atcctccgtg
    13321 gcatggcgaa gctgtatccc gcctaccggc tgatctcatg gctgccggtg gtgaagaaca
    13381 tggcagagaa aattctgatc tttcaaaagg attaagggca ataccgcaca aagattgtgc
    13441 gacagatgcg tcgtcaaatt tttcgtc
//
LOCUS       DAWBGR010000056        13444 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1440_length_13444_cov_3.051908, whole genome shotgun sequence.
ACCESSION   DAWBGR010000056 DAWBGR010000000
VERSION     DAWBGR010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13444)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13444
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1440_length_13444_cov_3.051908"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8964..8973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3892 a         2774 c         2954 g         3814 t
ORIGIN      
        1 cgcctgtacg cattgttcgg aacgaaaact gtgagtcagt gtcgctggct ggtttgtatc
       61 catgcggcga aggagccgga tacgcaggtg gaatcacatc tgcggcagta gacggaattc
      121 gttgtgcaga acaagtgctg caggcatatt tgcaatgagc cgaaatatga cagggaaatg
      181 ctaatatgta attgcaccaa atacaaattt ggtgcaatta agaggaccca attacgaggg
      241 gagggcaatt attttggaaa ttgatcgcaa acagtgccct gatttttttc tggattatat
      301 tttatatatc actgtaacga aatcattgtc cagccgtacg gtacaggaat actatctgga
      361 cattcgtttg tttctgaagt atcttcgcat gatgcgtgag ccgcagtttc aggacatcaa
      421 cgacttgcag cagattccga tcaaagatat gtccgtatct gctttgcaat ctgtgacatt
      481 gcaggactta tataactatc tgtattatgt tacagaagaa cgtgaaaatc atgatcgtgc
      541 ccgcggccga aaagtttctg cactgcgtag ctttttccgc tatctctgcc aacatcagaa
      601 attgatttcg caagacccga ctgagcaatt ggaactgcca tcgccgaaaa aagctttgcc
      661 gcgatatttg acattggaac aaagccagca attgctgcaa tctttgaatt ctgcatatcc
      721 agaacgggat tattgcatat tgacattgtt cttgaattgc ggaatacggc ttagcgaact
      781 ggtgggtttg aatctttcag acatcaactt caaggaagcc aaaatgcgtg tgctgggcaa
      841 aggcaataag gaacgcatgg tgtacttaaa tgaagcctgt atggacgcac ttcacgcgta
      901 cttacaagtt cgtgagttgc ctgagggctc tgcggaaact gctgttttcc tgagcaagcg
      961 gcatacacgg atcagcaaac ggcgtgtgca gcaagtagta gaaactgcgt tggcacaggc
     1021 agatcttggc ggacaaggct tttctaccca caagttgcgg catactgcgg caacgctgat
     1081 gtatcagtac ggaaatgttg atgcattgac gctaaaagaa gtattgggac atgcaagcac
     1141 ctctacaaca gagatctaca cccatttgtc caatcagatt ttgaaacatg cgacagacag
     1201 ttcgcctctg gcacactttg aaagaaacag tcaaagtcaa gctgagcagg aaaacaatga
     1261 agatcatgaa tcgaaatcag agaatacaga tattacaaat ccagagtgaa ttactaatac
     1321 ataacattga tatgtattgc taaaacaacg ggcaagttac aataatcatg taacttgccc
     1381 gttgttttta tatcaatttc aaagtacttt attcccatgt aaactgtgtc agttcgcctt
     1441 ctacagataa ctctggatat tcccattgcc gtaatacttc atatgctgca attgccacag
     1501 aattggaaag gttcagactg cgaagggtat ttcgcatagg aatgcgaaca caggtgtccg
     1561 gatgttgtac tagcagagat tcgttcagtc ctttgtcttc tctgccgaat accagataca
     1621 caggtttatc tgttggataa gaaacatcac tgtgaatatg tctgcccttt gttgtgaaga
     1681 aatacctctc cccttccgga tttttgtcgc aaaagtcctc ccagttcgtg taatatgtca
     1741 agtccagctt gtcccaatag tccaaccccg cacgcttgag gtgcttgtct gtcaccgtaa
     1801 atccaagagg ttccactaaa tgcagccttg ctccagtaac ggcacaggtt ctggaaatat
     1861 ttccggtatt ctgcggaatt tgcggttcga ctaaaacgat gttcaattgt gataatttca
     1921 taaaagccct ctcttttcca gttgtggaat ggatatacag ggataaatgc agttttcttt
     1981 tcgcataata agcatgcagc agaaatcgct gaaaaatgaa aatgcggaaa ggagtatgca
     2041 aaaatgaccg taaaatctgt agttcgcggg gttacagttg gtgcaactgt aggaacagtt
     2101 tgttatatga tgtccaaggc aacctctaag cagaagcatg acattcgaaa aaatgccggt
     2161 aaggcagtca aagccgctgg ttgtattctg gacgatctca cttctctgat gtggtaaatt
     2221 caatagtatc gctatcgcat agaaatcacg cagctttttg aaactgcgtg atttttgctt
     2281 tttagatttg aggcaatacc gcacaaagag cgtctggcga ctttgcacgc catctgcatt
     2341 gccttgactt agaatatgtt tttatttcag ctggttgccg tcagaaaatg cggtgccaac
     2401 tttttcaccc agtttgcgat aggtgtgagc aacgctgtcg aaaacaatcg gatctacctt
     2461 gtcagtatca atctttccat tttcgtccag cacacattcg tcagcacaaa cattgacaat
     2521 ttcgccgaca acaatgccgt cttcattgaa ctttaccagc ttgcattcca atgccatcgg
     2581 gaattcctga aacagcggag catcaactgt agcactcttt tctgcgtgta gaccggactt
     2641 ttccatttta tccggtgtgt tatttccgga aaccaaaccc acataatcag ccggaacagc
     2701 cgtatccgca gttgcaaagc tgactgtgaa agaacccttt gcccgaatat tctttgtcgt
     2761 cttgtgactt tcacttaagc aaagcatgat ctgattggtt tcataaatgc cgccccaagc
     2821 tgcattcatg gcatcgggcc ttccgttttc atcataggtg ccgacgatga gaaccggcat
     2881 cggatacatc catgtctgtg ctccaaaatt ttttctcatt tgtaagactc ccttttcgat
     2941 aaaatccggc aggacaaatc ccgccttttt tattatagca caattttagg ggattgtcca
     3001 gctgttcaaa atacattttc agaaaaacag caaatttgca aacttgctaa aattatggta
     3061 tactggggat tatgatatag agcatattta cataaaaatg aatagcaccg ttgaaatgca
     3121 ttttgatata tcatagaatt ttatagttca aaaaatattt acaaatttaa acgcacattt
     3181 ttccagttca aacgttaatg ccaatgagag gggtgagatg cagtgcctat gaatctgtcg
     3241 cagcaatatg acaagatcta tcgatattgt tttttcatac cagatgtcag gaacttgcag
     3301 aagatttgac gcaggaaaca tttttgagat actatcagca tccgcagtat caaagcaccg
     3361 gaaaagaatt gcagtatttg tatgtaattg caagaaatct ttgtattgat acataccgca
     3421 agccctctgc cgaacccctc agtgaggaaa atatgccggt tgtttttgta caagatgatg
     3481 attttatcac acatgtagca ctgcgaaatg ctctttcaga attgacagcg gaagaacggg
     3541 aactgatcct gcttcgttat gtcaatgaag tgcctgtcgc ggtgatgagc cagctgtacg
     3601 gaatatcccg ctttgcactg catcgaaaat tgaaagccat actggcaaaa ctgcgaaaac
     3661 attttggaaa ggaggcagac gggtatgaaa cggaaatata aaaaggcact gcaagactgt
     3721 tttgaaccgc ctgctcctca aagaaaagca cagtttctac agcaaataca ggctgaacaa
     3781 tcacaagata ccgccactcc cctctttttt cagcggtatc cgaagtggat tcgacagtct
     3841 gcaatggtgt tctgtgcagc tgcggtactg ggtattgggt atggtgcgta tcaagcgttt
     3901 ttcgattcca gagatcaact cgaggttgtt ccgccgcagg aaaccacaac aacagcctgg
     3961 atagatccgg caacaacgac tgtaacttca caatcaatga catcatttac ggcagaaaca
     4021 acacgaacaa cagttactac cggtgaaaaa aattctgctg aaacagtttt agcaaccgca
     4081 gaaaatactc tgtcaagttc aaccagtaca gtatccaaac ataccacctc tcaccaagta
     4141 ccggaaaaat cgacttcgga ttccaagcaa actacaatgg cggtttcaac tgtaagaaga
     4201 tctgatatga caacagcaaa agcagttgtg acttctgttg catatactac cacacggaaa
     4261 agaacaacga ctggcacaaa gcagatgacc actccccttg catcaacagc agttgcatca
     4321 gagcatacga ctgctgcaca gacaacaaca cgctatatag atggcaaaaa tgacatacag
     4381 accgttacaa cgtgtgctga ttcctgtgaa cccatgcaaa caaaagatta tacggtacag
     4441 ccgccatgca gttatacggt tgcaaaacac gttctggaag tttcccgggt aggtataaca
     4501 tttgacacac tttcagatct gattgcaaaa tcggatacga ttgtactcgg cacaacaaat
     4561 gctgtaactt ataccagtat tcagggacag ccgtggattc aagttgatgt caaactggaa
     4621 atggtttttg attcggcaaa agagttgcat tacggagatt gcatttcaat ttatataaag
     4681 ggcggttata tgccaatgac ggaatatctt tcctatatag gagaaacacc ggaacaatgg
     4741 aatatgacgg cagagcaggc agcagaaacc agtctgtttg agaacaatgg agatatatca
     4801 cttcctaaag tcggagaaac gctcctatat ttcttgaatg atggcggaga tgcggtgcct
     4861 gacggagcat atgttcgata ctcggaattc tattggaaaa acggcgtata ctttaattcg
     4921 gatgccaata acccgcttac ttttacgggc agcgaactgg tagatgccat tcacaaattc
     4981 aactgaaaaa tcacatgaat ggaggaaagc aagatgaaac aaaaatggat cgcacttgcg
     5041 gttttgggtg caatgcttgt acaggttgct cccatcagca gcatgactgc cgctgcacta
     5101 gaaacaacgg ctatttctga tataacagca acagatgctg tattactgaa atacaacaaa
     5161 catgatacct atgtggagat taccggattt cgtgcagatg aaacagacat tgtgctgccg
     5221 gataccattg acggtctgcc ggtaacgacg attcaagaag gggccttctt ttcgagtcag
     5281 ttaacttcta tttatattcc ggagagtgtc acccagatcg gcggaattgc ctttcaagcg
     5341 tgtgcagatt tgcagagaat cgaagttgat gagcataatc cggcgtatac cagtgtagac
     5401 ggcgtgctct atgacaagga agaaaaaata ctgtatgctt atccgaatgc tcttgcagaa
     5461 aatgaaaaca cactgaaaag ttcgtattct gtgccagacg gcgtggaaac gattgcacag
     5521 ggtgtatttt tctacaacca atccttgact aatctgacct tgccgcattc gattcaggtt
     5581 ctggaagctg atgctttctc cgatactgca ttgcgggata tatattatga cggaacacag
     5641 gcagactgga atgcgattgc aaaaaaggtg cctactggtg gaaatgtgga acagccgttt
     5701 cgagaattct atgcggagat gcactatctg cgagaaaata ctttcacaga aactactgct
     5761 gcaattacaa caatccagac aaccacagaa tctactgaag gcacaagtca aacgacttca
     5821 gaaatcacta catctatacc agtttctact gcaactacaa cgcaggagca gcggacttct
     5881 acggaaagcg aaaccgcaac cacaataacc accacctcaa ctgcctctgc ttctacagaa
     5941 accagtacca gttcttccac caaacaagaa gttcctgctg atgctctcta tggggatatt
     6001 gatttggacg gaagaattgc cattaacgat gctgttctcc tgaacaaata tgtggctggt
     6061 gtcgttatac tgaatgcaca gcagacgcag aatggggatt gctatcagga ttcttcctct
     6121 ggaacgatcg atatgcagga caccacttcc ctactgcgat ttttggtgca tatcaccact
     6181 accctgccgg aacaggatac atcgcagaag ggaacactta cgcctaccac agcaattcac
     6241 aatgtctatt tcaaaaatga atctgaaata gactctatgc cggcagtgat ttccacaatg
     6301 gacgaatgga atgcatatgg ggaacagttt gccgtggaat taggttccaa aaatagtctg
     6361 cttaacacag aaccattctg caatcaatat acagaagaat ttttccaaag tcatgtgttg
     6421 ctggtcaata tctcaccggt agtttctgcc ggaacacagg agttcttttc cattgacgat
     6481 gtttttcata gtaccgatgg aaaaatacac ataaaggcat cgaatcgttc tggcatgctc
     6541 atggattgtg ttggtatcgg cagcagaatg ctgcttcagg tgacgattcc gaaaacacag
     6601 tatcacggtg aagaaattgt atgggatacc aattggattt ttccgtcgta tgatatgctg
     6661 ttcaatatta ccacagcaac agaacagggc tgggacaaag caaagagttc cgattccgca
     6721 ttgatttttt ctactgtgga agaattagag aaacaaatta catcgctgtt gggagaaaaa
     6781 acaacagaaa atctgaaaaa atactatgat tcggagttct ttgcttccca tgtgctgttt
     6841 gttgatctcg ttgcaaaacc agacggaaac gactatgaaa atataatttg tgaagtttca
     6901 caggataaaa ccggaacgct gcagatcact tatcaacagg aactttgtaa cggttttcat
     6961 tcaaaatcta ttgatctgct tttggttgct ttgcccaagt cccaatggaa tggaaaagca
     7021 ccggtgtgga attctaaaac attggacaca agcagctgtc aactttcagt ggaacaaaat
     7081 attcaagcag ttaccaaaag cggctggaat cgtgcattga gcagccgtcc ggaaatggtg
     7141 ctgacatcgg agcaggactt tgaggatagc ctcgctcctt tgtatgaaaa tgcagttgcc
     7201 cagcaattgc gtgactattt taacgatgcg ttctttgcag actatgtgct gtttgcacgt
     7261 ccgactgtaa aatgcaattc tgtagtctat gcacccgaaa aagttgtgca gcaggacaat
     7321 ggctacattg agatttttta cagtggaaca gaaattgacg gcacctccgg tactggaatc
     7381 gacttgcttt gggttgcagt tcctatagag cagtggaacg gaaaaaaacc aacatggaaa
     7441 gaagtcggag aaaccgagat ttctgcaagc ttgcgatatt atgatgtatt gaacatttct
     7501 tccatgaaaa aacagtggat ttcttcaagg gaagaattgc ggacggtgct tcaaagtgca
     7561 aatatcacag atgaatacat cacagccgat acattgctgc aattatatcc ggaaagcttt
     7621 tttgagaaaa aagcacttta cttatctgta gatgatgata ttgcaggcac cagtcgaact
     7681 gtgttccggg ttacccaaaa tgaaaacact cgtaaaatca cagtatcctg tcgaaaaaaa
     7741 cagcctgtat cctgcgacgg agttatgcag atcagcatgc ttgcagtttc caagaatatt
     7801 gcagagaatg cagaagttac ttatgcggat tataatgatg agtttttgct gaataatgga
     7861 gcatgggcat ttacgacaat tccggatacc atgggagaag ggtttgctgt caatcagtat
     7921 agctttggaa attacagcga agtgattttg tacagtacct cgccgtctgg catagttcta
     7981 tatcacaact ttaatcggtt ggcttccctg ccgctggacg aagcgttttc cgtgttctcg
     8041 gattctcagg cagtctggac aaaagacgat tccggaaata atgtgtgctc ctttggcaat
     8101 ttgcagatta ccgttcggga aaaagaattg gaagtttctt atccttacaa taaaaccggt
     8161 gacagaaaaa ctgtgacaat tcctcttgct gaaatcgttt aaggcaatac cgcacaatct
     8221 ttgtgcggta ttgccttaag caaaatgaca gaataaaatc aatctttttc gtacagcgtc
     8281 gctctgcaaa acagggcggc gttttatgat atatccatag gatttcattg tgatttttca
     8341 taatattcat tgacaattct acagaaatcg tgtatactaa atagagtatg ctcacaggag
     8401 tgataaaaga aatctttgtg aacgcacttt tgtgctggtg tacagaaaat tgaacttgtt
     8461 gatattttca tattagcatg cttcaagtga aaagagagga gtattttatc atatgcaatc
     8521 aaaaactaca caagtcaaaa gcggatttgg atccaagctg ggatttattt tagcagctgc
     8581 gggctctgct gttggattgg gaaatatttg gcgtttccct tatctcgccg caaaatacgg
     8641 cggtggaatt tttctgttgg tgtacatcgt gctggccgtc acgtttggct ataccatgat
     8701 cgtggccgaa acagcgcttg gccgcatgac caaaaagagc ccggtggggg catttggcac
     8761 attcggcaaa agcggcaggc tgaaattcgg cggctggatc aatgcagtca ttcccattct
     8821 gatcgtaccg tattattcgg tcattggcgg ctgggtcatc cggtatcttt ccgcatatgt
     8881 cagcggccac ggcagtgagc tggcacagga cggctatttt tccggcttta tttccagcgg
     8941 tctgtctgca gaggtgtgtt tccnnnnnnn nnnaccggaa aaagtgtcat acaggcatat
     9001 tcagatatga actgtcattt caaaccgctt ggttggctag caggagcgat tccggtaatt
     9061 atccttccct actactgtgt gatcggaggt tgggtgctga aatatctggt gatatttaca
     9121 actggttctg gagcaagtgc ggccggtgac ggttcttatt tctctaactt tatcggtcat
     9181 gccggatcac cgatgttgtt ttttgcaatt ttcattctcc ttaccgcagt agttgttatg
     9241 ctaggtgtgc aaaaaggggt agagaaagtc agcaagctaa tgatgccggt attgttgatt
     9301 ctgattgtag gaattagtat ctatacgttg actttgccgg gagcaattga tggattgatc
     9361 tactatgtca agccggattt caaccagttt tctataaaaa cagtaattgc cgcagtagga
     9421 cagttgttct attctatgtc attggcaatg ggtattatga ttacatatgg ttcttacatg
     9481 cggaaagaag ataacattga aagttctgtt cgccagattg aagtgttcga cactttggta
     9541 gcttttctgt ctggactgat tatcgttccg gcagtttttg tcttttccgg tggtgatgaa
     9601 aatgctttga atgctggtcc aagtctgatg tttatcacac tcccgaatgt tttcaaggaa
     9661 atgttgggcg gacagatcgt tggtgcagta tttttcttgt tggtggtact ggctgcaatg
     9721 acatcttcca tttctttgat ggaaacagtt gtttccattg tggaagaacg attccactgg
     9781 aaacggattc ccagttgcct gattgttacc ggcatatcgt ttctgatcgg tttactttct
     9841 gtctttggat atagcatttg gtctgatttc actattttcc acatgcagtt cctggacttc
     9901 ttcgactata ttagcaataa cattatgatg ccgattgtcg cattgatgac ttgtgtgttg
     9961 gttggatttg ttgtaaaaac aacttttgta gaagatgaaa tcgagaagaa tgcacgcttt
    10021 cgtgtgaaag gtttgtatcg tgttatgatc aaatttatct gtccgatttg catgctgttg
    10081 attttattct ctgctctgtt cctgaaattg taatttctat ttttacgtga aaaacgctct
    10141 ggaaagcgga ttaaacaaag ctttccagag cgtttctatt ttttgttatg gaatgtattt
    10201 actgaacaat cagaattact gttccgtcca agtatcactg aaaatggctt ctccgttagt
    10261 gttaattaca tcgcggtaga aataaccaga atcctttatg gtacgctttt gtgtctgata
    10321 atccacgtgt accaaaccga aacgcttatc ataaccgtca gcccattcga aattgtccat
    10381 gacagaccag tacatgtaac ccattacctt tgctccgtcc tgcatcgaac gatacagttc
    10441 ccgcagatac cgtttcatga aatcaattcg ctgcggatca tgaactttac cgtccagctg
    10501 tacccagtca tggcttgcca taccgttttc actaagcaga atcggtttct gatagcgttc
    10561 atataggaac aacggactcc agtacagtac ctcaggtgtt accggccaac ccatttgtgt
    10621 tcttgcacag ccgatataag cgttttctgc atagccaaat tctgtttccg tagccttgct
    10681 ctcataaata ttgacaccgt aaaaatcgac cggctgagag atgatttcca tatccccttc
    10741 cagaatttcc ggcatgtcgt ttccaagaat ccgataagct tcttccggat acttgccaaa
    10801 gaagatcgga tctgcccacc aagcaagttc aaatgggaag cggtttttag taaaagcgaa
    10861 actcttacgt tttgcttctg caatggcttc cggtgtattg ctttccggtg tgcagcaggg
    10921 tgttgcaaag gcaaatccaa cagttggctt ctgttttgca tacttccgga tcacctgtac
    10981 tgcttttccg tgagccagca aaacgttgtg tgtcatgcga gtcaagtcgt gtgcctgatg
    11041 cacctcgaaa ggagcaaatt ttccaatggc atagccgcag ccgatgaata cttgcggttc
    11101 gttaatggtc atccaatact ttacacgatc agacagagca tcgactacga ctttggcata
    11161 ttcagcaaac cagtctgagg aatcacgatt cagccagcca cccttctgat gcagtgccag
    11221 aggatagtcc cagtgaaaca gcgttaccaa cggttcaatg ccgtttgcca gcagttcgtc
    11281 taccaaatca gaatagaact gcaatccctt ctgatttact tttccagtac cgtccggcaa
    11341 aactcttgtc cagctgattg aaaaccggta attgcgaatg cccatttttt tcatcatttt
    11401 cacatcatct ttgaagtgat gatagtggtc gcatgcaata tttccgtttt cgctgtgtgc
    11461 cacatgaccg tcttcctgtg tgtaagcatc ccagacgttt aagcctttgc catcgtcttg
    11521 atatgcacct tctacctgat atgcggcgga agcagttccc cataaaaaat tgtcctgaaa
    11581 tttcatatat aaacctccta aagttgatag atcaatggtt gacagattgc ctgttctgtg
    11641 ttatattata tctgtaacaa tccgtaaaat ctagtactat atcctcggat tcttgtactt
    11701 tttccactaa ttttctctga ttgtcaaaat gcagagaaaa aaagaaacag aggtgtcgga
    11761 attggaaaaa cagaaatttt atgagaagcg aaaacatggt accccacgat tccctatgga
    11821 atgttttgtg acacgtgcag atggaaatca acattttctg gtaccgttgc actggcacag
    11881 aaatatagaa attatgcaaa tgttgcatgg tacagcagta attacaattg gaaatgaaac
    11941 gttttccgca gtagagggag atattttctg tatcaatcag gaggaactgc accgaattgt
    12001 ttcagaggat aatcagttag tatatcgtac agttattttt ccgttacagg cattgacttt
    12061 ttcagaaagc gatgctgctc agacttactt ggaacaaatt gtacaagggg tactgcggtt
    12121 tccggtacaa gttaccaatg aaactgaaaa atattttctg cgtgaaacct tgtatcagat
    12181 tcaaattgcc gcggagcaaa aaagtgttgg gtatgaactt atggtaaagg cacagctttt
    12241 gcaaataatt gcacaaatgc tctgccaaca tcagattgtt tcggtgcagc attcccccag
    12301 tgaaaaaagc aaacgcttga aagaaatgtt gcattttatt cagaggcatt atgctgctcc
    12361 tttgaccatt acagttatgg cagagcagtt tcatatgtct gaaaaatatt tttcccgcta
    12421 ttttcgcatt gctacaggtc aaaactttac ggcatattta aacgctgtcc gaattgaaaa
    12481 ggcacaggca ttgttactgg aaactgatga aactgtttta gaaattgctt tttcttgtgg
    12541 gtatgaaaat gttagctatt tcaatcgggt atttcgcagg cagacagggt attccccatt
    12601 gcagtatcgc tcgatagcag tatcttgaca aattcataaa tagtttacaa tactacctat
    12661 ttacattctt acccaaaagg attataataa aggcagcaca gcacaaggtt tatttgtcga
    12721 ttttgcacgc aatttaccaa gtgcatctta cgtacaagct ttgtgcagtg ttgtttgaaa
    12781 ttggaaatca tgtagcggtg cacgattgca gcaacatgta tttccagaaa gaatattgtc
    12841 aaaagagttt gctgggaaat gctccggaaa ctcgtgtaca ctggaggtat ttgtatcatg
    12901 ggtagattta cattgccaag agatttgtat catggcacag gttctttgga aacattaaaa
    12961 acgctgactg gcaaaaaagc aatcgtagtt gttggcggtg gttccatgaa gcggtttggc
    13021 tttttggaca aggcggtggc ttatttgaaa gaagccggca tggaagtgga gctgtttgaa
    13081 ggcgttgaac cggatccttc tgtagaaacc gtaatgaagg gtgcagaagc aatgcggaaa
    13141 ttccagccgg attggatcgt tgccatgggt ggtggctctc cgattgacgc tgcaaaggca
    13201 atgtgggtat tctatgagta tccggatact acatttgaag aaatcattac accgtttagc
    13261 ttcccgacat tgcggacaaa ggcaaaattt tgtgcaattc cgtctacatc gggtactgcg
    13321 acagaagtaa ctgcattttc ggtaattacg gactatcaca agggtattaa gtatccgttg
    13381 gctgacttta ggaaacactg attaaatctt cccattacgc agcaaatatg gtataataga
    13441 aaaa
//
LOCUS       DAWBGR010000057        13314 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1450_length_13314_cov_4.740026, whole genome shotgun sequence.
ACCESSION   DAWBGR010000057 DAWBGR010000000
VERSION     DAWBGR010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13314)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13314)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13314
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1450_length_13314_cov_4.740026"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10338..10347
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3068 a         3601 c         3556 g         3079 t
ORIGIN      
        1 gacgaaaaat ttgacgacgc atctgtcgca caatctttgt gcggtattgc cttgaaaatt
       61 ttaaaacagc actgcacaaa gctttgatgt cagactgcgt gcaaagccca cagacgggtt
      121 tcgtgcggtg ctgcctcaac atacagaaag gatcatcact atgatttatg tatttcttgc
      181 caatggattt gaagaagtcg aagctcttgc accggtggat atgctccgcc gtgccaagca
      241 ggaagtgacc actgtaggcg tgaccggaaa gatcgtcacc ggctcacacc acattcccgt
      301 gaccgcagac atcaccgcag aggaattgca gatcggcggc gatatggata tgatcgtgct
      361 gccgggcggc atgccgggca cgctgaacga ggaagcttct ccggtggtgc aggctgtcat
      421 tgactattgc gttgccaatg accgctggat cggtgcgatt tgtgctgctc cctctattct
      481 gggacacaag ggcgtgctga acggaaagac tgctacctgc tataccggct tcgaaaagga
      541 actgaccgga gcggagatcg gcagcgacgg tgtcattcgg gacggcaaga tcattaccgc
      601 acggggtgcc ggcgtggcag tggatttcgg tctggaactg gtgggagccg tatgcggcag
      661 agaaacccag cagcagattc gcgggtccat tctctgtgat tgagcagatt gcggcaaaac
      721 aggctctccg gcaggaaatg cgtcgccgcc gcaaagcaat gccgccggaa gaaaaagccg
      781 caggggaaca gcggctttgc cgccggtttc tggaaagttc gtattaccgg aactgccgcc
      841 agctgttttg ttttgttgcc ctgccgcagg aaccggaaac acggggcatt ctccggcagg
      901 ctctggcgga cggtaaaatt gttgccgtgc cccgctgtct gccggaacgc cgcatggcat
      961 ttcaccagtt gaacagggaa gtgccgctgg aacagcagct gctctccggc agcttcggcg
     1021 tggcagaacc gcaggaaacg ctgccggtca tcacgcccca gccggagcag gtaccgctgt
     1081 gtctggtgcc ggggctggcg tttgaccgaa agggcggcag actgggctat ggtgccggct
     1141 attacgaccg ctttctggcg gcgtatccct ttttgctgaa aatcggctgt gcactgacac
     1201 cgtttctcac ggactgtgtg cccacggaac cgacagacca gcgtctggac ggtattgcta
     1261 cagaatcttc gctggaggta tggaatggat aacaaagact tcaaccgcga tcaattgctg
     1321 aatgagatcc tgcgggaaac ggcagccccc aggagccagc cggctcctca gcccgcaccg
     1381 gcacgggatc cggaaccgcc aaaaccggct cccgctgctc cggcagcccg gccggcaccg
     1441 gcagaaccgg cacgccctgc ggcagcaccg cagacacagc agcagccggc tgcggaaaca
     1501 ccggcaccgg agcccgacaa ccgcactgtt cttttcaaca gtgcccggtg gaatacggat
     1561 caggtgttca ctgcccgaca gccggtattg gaaaatgacc cgctgtatga agatctggaa
     1621 ggggagagcc agccgtttcg tccggaatcg gtacatagca ccactgccaa caccgccaag
     1681 cgtccccgca aaaagcggaa acagcatcgt gtcatcagtg cactggtaat gatcattatc
     1741 atcattggcg tgtccattct gctgtccagt gccctgatcg tctacgggcg tgatcttctg
     1801 ggcatcaaca gcgacagttc caccaagatc gttaccattc cgcagggtgc caccatgaaa
     1861 gagatcgcag ggactttgca ggattcgggg atcattacca agccggactt ttttgtcttt
     1921 atcgtgggca tgagcgacaa ggacaaggac atcaagcccg gcgaccatga gctgcggccg
     1981 gacatggcgt atgaaaccat tctgagcgag ttgatcagtg atcccatgga tagttcgctg
     2041 tcggtatctg tgacatttcc ggagggaatg cgtctggtag atgccgcaga tctgctggag
     2101 gaaaacaacg tctgcgatgc tgacgaattt ctggattact ttaaccacag tgcgaagttc
     2161 ggctatgcct atgaggacta tctgcccagc ttccaggacg aaaagttcta tcagatggag
     2221 ggctatctgt tccccgatac ctatacattc tatcaggaaa tggacgtgga actggtctgc
     2281 cagaagattc tggggaactt caacgacaag atcaccaagg acatgtatga ccgcatggac
     2341 gctctgcata tttcgctgga tcagaccatt acgctggcat cgatgatcca ggcagaggca
     2401 gggaacaccg agcagatgac gacgatctcc tccgtattct ggaatcgtct gaacaatgcc
     2461 accgagtatc cgctgctcca gtccgacccc accaccaagt atgtggagga agtcatcaag
     2521 cctcacagcg aatcctacaa tcaggatctg tatacctctt acgataccta tcagtgcacc
     2581 ggtctgccgg ctggcccgat ctgcaatccg ggcagtgaag ccattcacgc ggcactctat
     2641 ccggacaaca ccaactacta tttcttctac tccaacattg acaccaagga aacctacttt
     2701 gcggaaacct tgcaggagca cgaagccaac cagcagaaag tgcaggaaca gcaggaagca
     2761 gccgcagctg cggaaagcag cgaggaggaa agcaatgaca cagaagaata aaccggaact
     2821 gctggcacct gccggcgatc tggaacgcct ccgcggggct ctggattacg gggcagatgc
     2881 ggtgtatctg ggacgggaaa gcttcagtat gcgggcggcg gcaagcaatt ttcacagtgc
     2941 ggagcagctg cacgaagccg ttgcactggc acatcagtac gggaaaaagg tatacctcac
     3001 ctgcaacacc ctgccccgca acgaggaaat tcccacgttt ccccagtttc tgcgggaggc
     3061 acaggaagcc ggagcagatg ccctgattct ggcagacctg gggcttctgg caacggctcg
     3121 gaagctggca ccggatatgg agatccacat gtccacccag accggtattg tcaactatgc
     3181 cacctgtcag gctctgtatg atatgggcgt gaaacgtgcc gtgctggcac gggagctttc
     3241 gctggaggag atcgccggta ttcgggcaaa gattccggcg gaaatggaac tggaagtgtt
     3301 tgtccacggt gccatgtgca tgtccttttc gggacgctgt ctgctgtccg cctatctcac
     3361 cggacgggat gccaaccgcg gtgcctgtgc acagccctgc cgctggaact atcacgtggt
     3421 ggaatccaag cgggagggac agtatttccc tgtggaggaa accgatgagg gcacctatat
     3481 tttcaacgcg aaagacctct gcatgatcga ccatttgcag gaactgctgg atgccggcgt
     3541 ggacagcctg aaaatcgagg ggcggaacaa gtcagcgtac tatgtggcaa ttatcaccaa
     3601 cgcttaccgt gcggctctgg acgcggcaat ggcaggacag ccggcaccgg aatgggcact
     3661 gcgggaagtg gtacaggtca gtcatcggga atactgcacc ggctttttct acggcagaga
     3721 aaacgctgcc cagcgatacg ccgacagcag ctatgtccgg aattgtgacg tggtggcagt
     3781 ggtcaaccgc tgggaacagg gcagactgta cctcacccag agaaaccgct tttttgcggg
     3841 ggattctctg gaaatcgtgg ctgccggaca gccgccggtg acggtggctg cacaggggct
     3901 ggaaaacgag gacggcgaat ctatcgaaac cgcaaatcat gccatgatgc agtgctccat
     3961 tgcctgtgaa acggcattcc ccgcaggcag cgtggtgcgg aaaccggtgg aataaggagg
     4021 cgttttcatg cggcatacac aagcgatcat tctggcagca gtgctgcttg ccggtatcgt
     4081 gtccggcggc tgtacccgaa tccacagtgc agaggaattg acaaaggaca ccagaccgga
     4141 aaccacgccg gtgttcaccg atgccccgaa gctggaattc tccgcagatg gggtagaccc
     4201 ttacgccaat gtgatcgcgt actatgtgga acagtatcag aaagatctgg aaagtgacga
     4261 tatgcttcag ccgttcacct atgtgctcta tgacatggac aaggacggca caccggaact
     4321 gatcgtccgg acaggctaca gcgaagcgga ttatgccctc tacgtctgga cagccgatgc
     4381 ggacaaacag ccggtacagg tggagggctg tggaaacggt gcccataccc agatctacgg
     4441 ctcggaaaca gaactgggct ttttcatcaa cagctgtcac atggacacgg agtatattgc
     4501 acaggattat ctgacagagg ggaactcgct ggaagaagtg cagatctacg cctaccagat
     4561 ggagaacgaa gcacagtcgg cttacggcta ttacgatctc accgccggag agggcttttt
     4621 ccggctggac gagcagagca tcgaggacta tcgctataaa gccagcccgc aggtgaaaga
     4681 tgccaaaact gccctgtcgc tggaggagta tcagaaacag ctggaacagc tggacgatgc
     4741 tcacgctgtg ggataaaact ttcatagatc gtataaaacg cctcggcaaa cggtaaaaat
     4801 tccgtttgcc gaggcgtttt agcttaggca ataccgcaca atctttgtgc ggtattgcct
     4861 taatgtattt tccgcatctt ttccatagtg atcgctgtca cgatccaccg cagccacggc
     4921 agacacatga ccacctgcaa aaagattcgc cagggctttt tccagcccag acagggagca
     4981 caggcgatca gataccggct gccgtagaca atgcccatga catagggaat cagcagggca
     5041 atgagataca gcagcagggg cagagccatt agaagtgtca catatcccag catcacggct
     5101 ccgaaaataa ttgccggcag gaaaataatg ccgaacatca gtaccagcag tgcaaatatc
     5161 caccagaaac tgagcaggta cacgagcaga atgacaccgg aaaccagcat cagcaggaaa
     5221 aacggcaggg agagccacag caaggtcagc tgttgttttg ccgcctgctg cggggtgctg
     5281 ccggtttggg aaatgggtac acagctgcaa gtgcgtacca tggtatagca ccatgtcatg
     5341 gcacaaaatg ccattgccag tttgggcagg aaggacagcg gtgccggcag atcatacaaa
     5401 taccgcagga cttgcagcac aggattcgaa tcggtctgat ctgggaacag aacgtacacc
     5461 caaaccatca ctgccagcgg aaacagtgct cccaccagcg aaaacagatg cagcagtacc
     5521 tgtgagtggg tgcgttctgg gtgcagcttt ccggcaacgt ccagaatcac cagcacggca
     5581 cagccgatgc cgcagagaat tggcagcatg acttactccc gaatctgtcc gttgccactg
     5641 accagatact tcactgtggt cagttcgtgc agtcccatag gccctcttgc gtggagcttc
     5701 tgggtggaga tgccgatctc tgcaccgtag ccgaattcgc cgccgtcggt aaaccgtgtg
     5761 gaggcgttga catagacgac tgccgcatca acggttcgct ggaatttctg ggcagcatcc
     5821 agatcgcggg tgatgatgca ttccgagtgc ttggtgctgt acttggcgat atgtgccatg
     5881 gcttcgtcca gacttgccac cactttcacg gtaattttgt agtcgttgaa ttctgtgtca
     5941 tacagttctt ccgtcaccgg ctgaacggca tcgcccagaa tcttccgtgt ctgttcgtcg
     6001 ccgtaaatag tcacatcatg ggtgcggaat gcagcctgta cccgcggcag aatttctgcg
     6061 gcaacgtcct gatgcacgat cagcccttca atggcgttgc agacagaggg acgctgggtc
     6121 ttggcgttgt cgatgatgcg gacagccatt tcataatccg cttcgccgtg caggcagctg
     6181 tccacataga cgtgacagtt tccggcaccg gtttcaatga ccggcacggt ggactgccgc
     6241 agcactgcct gaatcagacc ggcaccgccg cgggggatca gtacgtccag atagccagtc
     6301 aggtgcatca tgtcggcagc agtttcacgg gaagtgtcct ccaccagttg aatgatgtcc
     6361 ttgggcagtc cggcagtttc cacagcgtcc cgcatgatct ctgccagtga ggtgttggag
     6421 tggattgcct ccttgccgcc gcggagaatg accacgtttc cggctttcag gcagagggtg
     6481 gcagcatcta cggtgacatt ggggcgggat tcaaagataa tgccgatgac acccagcggc
     6541 acacgggttt tgaggatctg caagccgttg gggcggacag agccgctgtc cacagagcca
     6601 atgggatctt cggcggcgat cagctgacgg gcagcgtttg ccatgccgga aatgcgggca
     6661 ggagtcagcc gcagacggtc ctgcagtgat tctgtcatgt ggttctgtac ggcagcctcc
     6721 aagtctttgg cgttggcggc gaggatttcc tcctgtcttg ccagcagggc atcggcaatg
     6781 gcagcgaggg cgttgttttt gacctgcggt gcggcagtgg caatggcgga ttccacagct
     6841 tttgccttga caccaagggt ttcagcgtaa ttcatgggta tgctcctttc tgagtggtaa
     6901 aagtgatggc atgattcata ataagaggat ttttttggtg cagctatttt tctaaaaaaa
     6961 tgctgccgag gaccagggcg agaagccctg gtcgctgtcc gcagacagcg aaatctctgc
     7021 gccgtgcttt tggcgcacgg agaaaagggt gagaaaatcg acagattttc gagggaaagc
     7081 gaacacgacc gctttccctg gctacccctt ttgtattggt gactctgtaa aacatcccag
     7141 cgggatgttt tacacggaag cgttctctaa tgatttcttt catgacagcg ggtactgcaa
     7201 aaaccgcccg tttttctcga atccatatgc ggtgtataac agcacgccca tttcagacgc
     7261 agtgatccac acggctccgc agccataggt tctggcttct tccaccacat gatccagcag
     7321 ttttcgggca atgccttgtc tgcgataggt gggattcgtg tacatgctgg acagcaatcc
     7381 gatttttccg gtgggattgc tgtagtacgg cggtttttcc acaaaggaca tgccgcttgt
     7441 cccgataatg cgttctccgt ccaccgccag ccatgacacg aaagtaccgt ctgccagatg
     7501 cttctggtag tatgctgtca gtgccggctc tatgtccaga tcagactttg ccccttcttc
     7561 ttttagctgt gcgattcgca ttgtcacaaa tgtgggaatg tctgcttctg tcagacggcg
     7621 gtaggtcagt aaggcgttca tgggtgtgct cctttccaga tgataaaggt ggataagaag
     7681 attttttagt gcagctattt ttctaaaaat gctgttcacg ttgatactgc accaagtctt
     7741 gtttcaggta tctcgtgaga aatattccac tggaatattt ctcataggtt tcttgctgga
     7801 aaagggtagt caggggaagc ggtcttgttc gctttccctc gaaaatctgc cgattttctc
     7861 accccctttc tccgcgtgcc gaaagcgtgg cacggggcgt ttcgctgtct gcggacagcg
     7921 attcagggct tctcgccctg gacctcgcca gcatttttga aaaaattgct ggagcaaaaa
     7981 actctattga tgcaatcact tgcgggtttc gtgtgataaa ttcattctgc cagaaaatgt
     8041 gttccgatcg gttcgccgtc aaagagggcg tacagctgtt ccggatctct gccgttcatg
     8101 atgaccatat ccacgccggc ttcagtggca atttttgcgg cattgatctt ggttgccatg
     8161 ccgccgctgc ccagtgccga accggcaccg cctgccattt gttcaatgtg ggcatcgatt
     8221 ttccggactt cccgaatgac ctgtgcattg gcatcggtgc gggggtcggc ggagtatagt
     8281 ccgttgatgt cggacaggat aatcagcaga tctgctccca ctgcggcagc aaccgtagcg
     8341 gaaagggagt cgttttcgcc gatttccagt tccagctcat cgatcgccac cgtgtcgttc
     8401 tcgttgacaa tggggatcac gcccatttcc agcagtctgc caatggtgtt ctgtacgtgg
     8461 ggcagacgct cgttggtcag ggtggcacgt gtcagcaaaa tctgtgccac ggtaacatta
     8521 tatttggaaa acatgtcgtc gtaaatgtgc atcagttcgc actgtcccac tgcggcggca
     8581 gcctgtttgg agggcgtgtc cttggggcgt tccggaaggt tcagcttgcc gacacccatg
     8641 cccaccgcac cggaggaaac cagcagcagt tcctttcccg cattgtggag gtcggacaag
     8701 atccgcacca gattggtcat gcggcgaatg ttcagttttc cggttttatg tgtcagcgtg
     8761 gaagttccca gcttgagaac aatacgcttt tttgaggata catctatcat tttggttttt
     8821 ctccttatgg tgcagtaatc acattctgcg gcgttccggc gagccagcac cgtaaatttt
     8881 ccgccgccag ttccagcagc cgctgtctgg tttccagtgg tgtccatgcc gcatggggtg
     8941 tcagtgtgca gttttccagc ccgaacagcg gcgtatctcc catgggttct tccgtcagca
     9001 cgtccagtgc agcaccggcg atcacgccct tttgcagagc ctgtgccagg tcggcttcca
     9061 cgatcagtcc gccccgtgcg gtattcacca gcagagccga gggcttcatg gtgctcagcc
     9121 gccgggcatt gaccatgccg gcagtctgtt ctgtcagggg cgtgtgcagg gtgagaatgt
     9181 ccgcctgccg gaacgcttcg tccagcgata ccagcggata ggggcagttc tggggctggg
     9241 ttcgtgtggc gatgcagagc cgcatgccaa aagcagcacc aattgcggca acctttttgc
     9301 cgatgctgcc gtagcccacc actgccagcg tttttccggc gagttcctgc atggggtagg
     9361 gcatgatgca gaaagtcttt gcctgtttcc acgcaccctg ccggacagcg gcatcatatt
     9421 gtgccacgcg gctgtagtgg tggaggatca gggcaaaggt gtgctgtgcc acggcatcgg
     9481 tggaatagcc tgccacattg cagactgtca cgccgtgtgc ttttgcggcg gcaatgtcga
     9541 tgttgttata gccggtggca cattcgccca cataccgcag cttcggacag cggtcgaaca
     9601 attccgccgg aatgggcgtt ttgttgcaga gaatgccgtc ggcatcgctc acacgttccg
     9661 ccagttgttc cggtgcggtg aggtcgtaga tctgcacttc gccaaactgt gaaaatatgt
     9721 ccagcgacac cccgccgccg gacagggtcg ctccgtctgt cactaccagt ttcatgcgtc
     9781 ggtttcctcg gttttgctgt cgccggcggt gtcggctacc tgtgcagcgg ctgcctgctt
     9841 ctcctttttg cgggcgatta tggacagaac aatggctccc agcagaagca ccagttcaaa
     9901 gatgcccacc gtgtggaacc agaccttgtt gtgttcgccg ccgacgtaga tcagcaggga
     9961 agcgacgatg cccactgcca gaagcagctg gtacgggcga cgcaggcgaa tgaactgcac
    10021 aacgaaaaag gctcctgcga tcaggcagaa gatcaggtga taggtgaatg aaatcggaaa
    10081 tgtacttgcg tccatagtga aaaaacttcc tttctgttga tacgaatcct gtttacggga
    10141 ttcccatgac cggcgttctg ccggatatac ccttctatta tagcatagat gatgctggtt
    10201 tgcaatgggt tttgtgcact ttttgggtgg ctgcccatag aaaaagccgc cggcagaatt
    10261 gccgacggct tgcgtttcaa acgaaagcag ttataaacgt ctgtttcgta ttttgtgtca
    10321 tcaatttttt tgagaaannn nnnnnnngac tctaatacat gctcaaaatc gccttaatac
    10381 acgctcaaag taacgtttgc cgttgttggt ccaacaacgt tgatattcac ggttcccgga
    10441 atagaatcca ttgtgagatc atagcgtcct tcttcaaaat aaatggtctt ttggattcct
    10501 gctcccataa atcccacgtc aaccagtgca gttagagcat ctgtattgtc cagaacgccg
    10561 tcaccgttca cgtccatcaa gttatagcat acttccgaaa attcgtcgtt ataatccaga
    10621 acagaatcca gaagatctgc accctttacg ttggagcaag cgtttccggc accagatctg
    10681 gcaacacaat acaactggaa gtacaccgtt actacgtcca gctgagatgt cttatccatt
    10741 tgtcccagtt catacacctg attctcatgg atccagtctg catcgctctg gtcaacaaca
    10801 ccgtcgccgt tcacatctgc cagcttcaac tgctccgcag tcaaagcctc gttgtccaca
    10861 tacagtgcat gtgtcaccat tgccgcatcg tgtccggtga taaaaccgtc catgtctaca
    10921 tcgcccagtg catacgctcc ctcagcactt gcactcatag cagataccgg cagtgctgcc
    10981 agtgccataa cgcccgcaag gactgcggaa ataccctctc tcattttcat aaaaattcct
    11041 ccttcaaatt aaaaaaacgc ggaataaatc ttgcacataa ctgccttaag cgttttctac
    11101 accataccga atgctgtgtt ctacaccagc tttgatctga tcccagacat tttctctctc
    11161 gccagctgca tcggcatcca cccaagccag caccagatct tcacccagat cataccgctt
    11221 ttccgtgaaa taaatggtgt ccatttcaca tgcacctcta agagccacat ccaaaacagt
    11281 gacaaacaca tcctcattat ccaaaacgcc gtcaccattc acgtccatca agttgtagca
    11341 gacttccggc aaagcatctt catagtaccc gttttccaaa agagccacgc ccttttccag
    11401 aggacgtgcc ttgcccgcac ccattctggc aatagtgtat gactgttcat aaatcgtgca
    11461 tgcattcatc gtaaacacgt cgatctgccc cagtgcatac acctgattct cgtgaatcca
    11521 gtctgcatcg ctctggttaa caacaccgtc accgtttacg tctgccagcc gcagctgttc
    11581 cgcagtcagt gcttcattgt ccacatacag tgcatgtgtc accatagccg catcgtgtcc
    11641 ggtaatgaaa ccgtccatgt ccacatcgcc cagtgcatac gctccctcag cacttgcact
    11701 catggcagat accggcagtg ctgccagtgc cataacgccc gcaagaactg cggaaatacc
    11761 ctttctcatc ttcataagta aaagcccctt tctggttgaa aatgcgataa actcctgccg
    11821 ttttgacaga catttatcta attttattat aatggttttt ttgtattttg tcaatctttc
    11881 aaccggtgtg attttgcaca aattttcaca gaaaaattca gcagatcata caaccaacgc
    11941 caaacaaaaa agcgttccgg caaaagccga aacgcttcca ttttcgttgt aaaaaacagc
    12001 ttagtcgctg acatacggca gcagtgcgat gtgtcttgca cgcttgattg cagttgtcag
    12061 ttcacgctgg tggaatgcac aagtgccggt gactctgcgg ggcagaatct ttgcacgttc
    12121 agtcatgcac ttcttgagct tggcaacgtc cttgtagtcg atacgctcaa cgcgatctat
    12181 acagaacata caaacctttt tgcgggggcg tttgccgcca cgctgagatc tttctctctc
    12241 catgatgttt ttatcctcct atctgcaatg cggtatttct ttggattcga tcagaaggga
    12301 acttcgccgt cactcagaat ttcttcaaag tcacccagat cgcccagctg catcgcaccc
    12361 ggtgcgttgt tcgctgccgg agcctgcggt gcaggctgtg ctgccggctg ctgatatgcc
    12421 ggtgcggact gtgcaggagc cggctgtgca tacgccggct gctgataggc accctgatac
    12481 ccgccgccat tttcctgtgc ggaacgcttg gactctccaa aggtaacatt gtctgccaga
    12541 acttccatgc tgtagtgctt tacaccattc tggtcggtgt agtcattgtt tcgcagactg
    12601 ccttccacaa tgatcatact gcccttgtgg aaatagcggc tgacaaactc tgcctgctgc
    12661 cgccagcagc tgacgttgat aaaatcagct tcccgttcgc cggtctgctt attcgtaaaa
    12721 cgacgatcga tcgccagacg aatgcggcaa actgcaacac cgctcggcgt ctgccggaaa
    12781 tccggatccg cacacagccg tcccatcaga attactttgt tcagcatgtt gttccccttt
    12841 ctgaaattcg tcttacttta cggtaacgag agaacgcaga acgccgtctg tgatgttcag
    12901 aacacgctct acttctgccg gaacgtccgg agtggacgta aaggtataca gtgtgtagta
    12961 accgtccggc tcgtcgttga tcggataagc cagcttacgc ttgccccact cgtttacttc
    13021 gtccagtgtg gcagcagatg cgatcttctc gcggaacttt tcaaccagtt cctttacagc
    13081 ttcttcaccg ttcttcagac tgaaaaccgt catcagttcg taggattcct gtttctttgc
    13141 catactcaat acacctcctt ctggatttcg cccatgcagg gcatgagcaa ggataacata
    13201 cctattatac caaacccgac aaaaaaagtc aagccctttt gcaaaaaaaa ttgcacggaa
    13261 attctgcgtt gtcaatagat gtgacccatt ggatgaaaag atgccagaga taag
//
LOCUS       DAWBGR010000058        13250 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1456_length_13250_cov_5.281849, whole genome shotgun sequence.
ACCESSION   DAWBGR010000058 DAWBGR010000000
VERSION     DAWBGR010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13250)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13250)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13250
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1456_length_13250_cov_5.281849"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5737..5746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3163 a         3663 c         3449 g         2965 t
ORIGIN      
        1 acgagcgaat tccgccggac ggtttcggtg tggctgcact agaaagtaca ctttatgggt
       61 gaacccctcc accagcttcg ctggttcccc tcgtactgag cggtcccctc tgccctgcgg
      121 gcatctcccc accccgtggg gagtcacccc tttcagggga ggcttatttc aggctcccct
      181 gaaagggcac ccgtagggcg tgccttgtgc tgagctggca gccgcaggct gactgagggg
      241 tttcttctgc gaaacctgaa acatgagtaa aagatttcac caaaaccccg cccgtataat
      301 aaaaaaatcg ccctgcctgc ggagaatccc ccacgaacag ggcgaaatat tttcgttttg
      361 tcttgcggca gtgctgttac attgccgtcc ggttttccag tgccttgaac agtgtcacct
      421 cgtcggcgta ttccagcgaa ccgcctaccg gcagcccaaa cgccaaccgt gtgaccttga
      481 ccttcagcgg ctccagcagc tgtgccagat acatcgccgt ggcatcgccc tctacagtgg
      541 gattggtcgc catgatgact tcccgcacct tgccgtcctg caagcgtgcc agcaattccc
      601 gaatccgcag cttgtccggc gtaatgccgt cgatcggcga gatcagcccg tgcagcacat
      661 gatacacgcc ggtatattcc cccgtgcgtt ccagtgccgt cacatctttc gggctctcta
      721 ccacgcagat caggctgtga tcccgttctt cgtctgcaca gacagaacaa agttccattt
      781 ccgtgagatt ctgacagcag gggcaatagt gaatgtcccg cttcgccgac agcagagcgt
      841 tggcaaagcg ttccacgtcc tcctccggtc tggaaatgat cgaatacgcc agacgctgtg
      901 ccgatttcag cccgatgccc ggcagttttg caaactcctc cgccagaagt tccagcggga
      961 gtgcgtgata accagccatt ctgttccctt agaacaagcc cgggagagaa acaccgccgg
     1021 tgatcttgct catttcagat tctgtggttg cctctacctg attcactgcc tcgttcacag
     1081 cactgatgat cagatcctgc aacatctcga tgtcctccgg atctaccact tccggcttga
     1141 tctccagagc ctttacggtc tttttgccgg taacggtaac gctgactgca ccgccgcctg
     1201 cggttgcagt aaagtcacgg ctctcgatgt cttcctgcag ttcggagatc tcgtcctgca
     1261 tcttctgtgc ctgacggatc attgcgttca tattctgggc accgccctga ttctgtttcg
     1321 gtaatcttgc tctcatgaga aaatctttcc tttcagtata aactcatatc ttttcgcatt
     1381 ggtaaaaatg cgggtttcac ttcatcactt acgccagcct gtcaggcagg cttctatcat
     1441 tcttccactg ccgtatccag atgactgtcc attgcctttt gcagcagatt ctgtgccttg
     1501 gaggtctgtt cctccggctc gctgcacttg gcacggatat tgtactctgc cccgaggaac
     1561 tgcttgacag tttcccgcag ggacagtgca ttttccttat tcttcagcag ggtgaggaaa
     1621 aaccggttct ttgccacgat cagcaggttg tttccggcaa cataagccga agactccgcc
     1681 agactgccgg acaccgccgg attcactgcc tgatatgccg ccagaatatc cgcccagttg
     1741 ttgaccggct ggatctctgc cgccgtcagg ggcttctgtt ccggttctgc cgcagcaggc
     1801 tcggctgctg gtgcagactg tgtggtctgg ggtgccgcag caggctgcgg ggcaggctgt
     1861 gccggctgag gagccgctgt cactgccggc gaaaccggta cagcgacctt gggcactgcc
     1921 agctgcaaca acagcatttc cagttccaca cgcttgttct gatttcgctg catgcgttcc
     1981 cgacagtctt gcagctgggc gatctgccac aggatcgtat ccagcgaggg cttctgtgcc
     2041 agctgttcca gccgcggcag ctcgtccggc agacaggtca gcagatccgg ctgtcccgtg
     2101 ccggctttca gcagcatcat attccgcagc tgttccagca gttcgtcaca aagccgcacc
     2161 atgtccttgg actggtcgta gagggcaccg gtgtattcca gtgcctttgc cgcgtcctgt
     2221 gcctgcactg cttccagaat ggcgaacaca ctgtcccgac cggcaacgcc ggctgccgcc
     2281 gtaacagatg ccgcggtgat cgcttcgccg ccggacgcac actgatccag cagggacaac
     2341 gcatcacgca taccgccgtc agacaggtga gcgatcagct gtgccgcctc cggctccagc
     2401 gaaagctgct cctcgccggc gatatattcc agccgtgccg caatgtccgc agtgcggatc
     2461 cggtggaagt cgtaccgctg gcagcgtgaa ataatggtcg ccggcacctt gtggacttcc
     2521 gtggtcgcca gaataaactt gacgtattcc ggcggttctt ccatgacttt cagcaatgct
     2581 ccccacgcac tgggagagag catgtgcact tcgtcgatga tatacacttt ataccggcag
     2641 cgttccggca gatagacggt gccttcccgc aggtcgcgaa tgtcctcgac gctgttgttg
     2701 gaagccgcgt cgatctccac gatgtcagaa agggctcccc gttctgcttc gcggcagatg
     2761 tcgcattcca gacagggatt tccttccggc gtgcggtgtt cacagttgac cgttttcgcc
     2821 aaaatgcggg cacaggtagt ttttcccgtg ccgcgggaac cggtaaacag gtaggcgtgg
     2881 gctgtctttt cctcacggat ctgattccgg agagccgttg tgatatgcgg ctgcgagatc
     2941 acatcgtcaa acgaccgcgg ccgccatttc cgatacaatg cctgatacaa cccttacacc
     3001 tcccagcaaa aaagattccg gaacataggt gtgcaggttt gctgcggcac acaaacacat
     3061 ccgcttaatg ctgctcggtc ccccgcctga catggttcac ggtgctttgt tgcacaggac
     3121 ccgcacacct atattccgga atccgtcctg atgtcaacgc atcagatatt tgtattatag
     3181 catactctta cagaaattgc aagtgttttt cgaaaaaaac cacagaaatt cctttcggcc
     3241 gtcagccaga gaaagcatac gtgaatatat atggcaacac cgcacaaagc ccgcctgacg
     3301 gctttgcacg caatctgcgt gcagactttt cctcttatcg ttcttccagc ggcacatatt
     3361 cctgcggctc tgccacagag agatatgccg gacggataat tttccggcag ttgatgagtt
     3421 cttccagccg gtgtgccgac cagccggcaa tgcgggcaat ggcgaagatc ggcgtgaaca
     3481 aatccatagg cagtcccagc atgcggtata caaagccgct gtagaagtcc acattggcac
     3541 aaacgccctt atagatcttc cggttctggg tgataacttc cttggcaagg cgttccacgg
     3601 tggcgtacag ctggtactca gcgtggcagc ccttttcatc ggaaagctgt tccacaaagg
     3661 atttcaggat cacggctctg gggtcggata cggagtacac cgcatgaccc atgccataga
     3721 tcagaccgct gtggtcgaag ccctcgccgc ggagcagccc cttgagatat tcccgcagag
     3781 cgtcctcgtc ctcccagtcg gggcagtgct gcttcaggtc ttcgaacatc tccatgacct
     3841 tgatgttcgc cccgccgtgc tttggtcctt tcagagagcc cagtgccgca gcaacggtgg
     3901 aataggtgtc cgttccggag gaggaaacca cgtgcacagt gaatgcggag ttgttgccgc
     3961 cgccgtgttc cgcatggagc accagacaca gatccagcag ttttgcttcc agacgggtat
     4021 atttgccgtc cggacgcatc atatacaaaa tgttttccgc aatggaaagc ttcggatcgg
     4081 gcttctggat aaacatgctt ctgtcgttgc ggaagtacat agaagcctga tagccgaaca
     4141 ctgccagcga cgggaactgg gacaccagct ggatacactg ccgcattacg ttgggcaggc
     4201 tggtatcatc gggcttgggg tcataggcat gaagtgccag cacgcacttt gccattgtgt
     4261 tcatgatgtt gtcactgggc atcttcataa taacgccgct gttaaagtga ttgggcagtt
     4321 cctgatagtc tgtcagcagc cccttgaact gtgccagctg gtcggctttc ggcagatgtc
     4381 ccatgagcag cagatagacg atctcctcga agccaaagcg gtcgtgggta ataaagccct
     4441 gcaccagctg ttcaatgggg atcccgcggt acaacagctg tccggctgcg ggaacttccc
     4501 tgccgtctac gatctgggta gcggacacct tgctgatcgt ggtcagaccg gcacgtaccc
     4561 ctttaccgtc cagatcgcga aggccacgct tgacttcgta ctttttataa agttcctgtt
     4621 cgatctcgta gtggggttct gcatctgcgg tcagctggtc taccagctgc atgtcgtgtt
     4681 tatattgtgc gatcatttgc tggatctttg tagaatgcaa cagtttttct tttttcatct
     4741 tatcagtcct ctcttgtcgg aacagcggtt tcgtctgttc gggctccata tcgttcaaat
     4801 tctgctgttg gaaagcagtg cataaagctc gtgtagagaa tgcaacaccc tatgaacatt
     4861 ctgcataata ccatgattga aaagttgatt tctttttatt atacaggatt tttgcagaat
     4921 tgtcaagttc gttggtgacg ttaaaataaa agtcacccgc ctgcaccaga aagcacctga
     4981 atatcaacat taccgatacc ggcaagggca acaatcggcg tcggcaagcc caggcggaca
     5041 gcgaggaacg agcgaattcc gccggacggt tttgacatga ccgcactaga aaattgctgt
     5101 cctcaggcaa gtccgcgggg cgatgtgggc gttctcccta cctatttcaa gggaggctcc
     5161 agtgtactta caatcacccc aaaaatgaac aaatcgcatc gagaaatcaa ggttgatttc
     5221 gatttgtaca caaaatcatg tggtttcttc gtgacaggta tcactttgtg tcacgaaatt
     5281 ttccggtttt tttcagtgct tcagccagaa acgctctttc gtgtcgcttt tgtaaatttt
     5341 taagcaaagt ccgcgggcgg cttgcaaatc caaatgctat atgataaaat agataaatcg
     5401 gaacgatgta tgtgcgattc cttgggcaat accgcacaaa gcaaaccgcg gcaccgcatc
     5461 gaaactcccc gaattttttc ggaatacccg caggaaatcc ctgcgaaacc atacgaaacc
     5521 attcatcaag aaaacggttt cagaatttga aagaggaaga agaagcatga atgactgtgg
     5581 catcagtaaa ctggatctga aacggcgaaa ccgcatgcag atcctgaaag ttgttcggga
     5641 acaggggccg atctcccgcg tggacatttc ggcaattctg ggcatcaccc gtgcggctgt
     5701 caccattatc accaacgaaa tgatcgacca gcacatnnnn nnnnnnttct acaggaagtg
     5761 ggcgaagagc cggtcaaccc caacgccgaa gtgcgaaagg gcagacgaaa gatcctgctg
     5821 gacatcaacg aaacctacaa gttcgcgatc ggcatctatg tggacgaaaa agaaatttcc
     5881 attggcctga ccaccctgaa cgcggcggcg ttggacaaga agtcgatcac gcacaaagcc
     5941 gacatgacac cggaagcact ggcagaactg ctgggaacca cactggaaaa aatgctccag
     6001 aacagctgtc tgacctacga aaacctgctg ggcatcggcg tgggcatcat gccctccgct
     6061 tttgaaatga tgggcggcag tacaacggac accggctgtc cggcatttcc tgtgctgcaa
     6121 aaggcactgg aggacagcac ccgcctgccg gtatacatca acagtgccgt caccgaattc
     6181 gccatggcag gcgtgcacta cggcgacctg catgccaagg cggaaaatca ggtctttctg
     6241 tactgggaca gcggcagtta ccgtgccatt cccgtcagcg gcaacgctcc cattctgcct
     6301 gccggctcca atgatgcctt tgtggaacac atctgtgtaa accctgccgg tgctcggctg
     6361 gagggctacc ccaagggcag cgtgcgggca gaactgtccg cggcagcagt ggcggaaaaa
     6421 atccgtccgg tcttttccga agaacagact ccgacactgt acgctgcact ggagagtgac
     6481 ctttcccgtc tgacaccggc ttccctgctg gctgcggcag aaacggacac cgccttgcag
     6541 cctgtcgcag acgaaactat caagcagttc tgcgtcatgc tccatacatt atattgcctg
     6601 tattttgcag agcatatcaa cctgtacggt ttcggcttca caccggagca tctggaacag
     6661 atccgcaaag ctgcggcaga attcgctggc aaggacttcg cacaggcgat cattctctgc
     6721 cccatcgggg aacggtatca cttcctcagc ggctgcacct atctgatcca gacaggattc
     6781 tatcagcgtg gcgggctgtg ctcgtaagcg ggtggcgaag aagacctgct tacaaaatct
     6841 ttgtaatttt agattcacgt gaaaatccct cagtcaccct acggctgcca gctccctttt
     6901 caagggagcc tgatttaaga agttacaata tgcgattcac ctgcacatat taaggaaaca
     6961 ctgaataaag cacctctcca tttccggaga ggtgtttttc attttgaaat ctgtctgatt
     7021 tttctgatac attctcaaaa aattaacttg tgttttttta cggagaacac ttgcacacca
     7081 gtgggagatg tgctataata atacttgcgg tatgcggcgg agaatccctg catcacgcac
     7141 gatcatatga tgtatcaaaa atggagagcc gatccccaaa acgacttctc caaatttatg
     7201 caaaaggagc agagttcgca ggggtgcgga ctgggtgaga aatcatgaga gtagcagaga
     7261 tgaatcgaga agcattggaa gcatttgccg ctgactgtaa aaagcagtac gaggcatttc
     7321 aggcacaggg gctgaaactg gatatgtccc gcggcaagcc ctcgccgaaa cagctggatc
     7381 tgaccaacgg cattaccaac tgcctgtccg aggacgacta taaggcagaa aacggtctgg
     7441 actgccgcaa ctacggctgt ctggacggtc tgccggaagc gaaggcattc ttcgctccca
     7501 tgctgggtgt aaagccggaa gatgtcatcg tctgcggcaa ctccagcctg aacatcatgt
     7561 actgggcaat gtccctcgcc atgaccaacg gcgtaatggg cagcaagccg tggggcaagt
     7621 atgacaaggt aaaggtgctc tgtccggtgc cgggctatga ccgccacttc gctgtcagcg
     7681 aatttctggg catggaactg gtgaacgtcc ccatgaagga agacggtccg gatatggaca
     7741 tcgttgaaaa gctggtggca gaagacgata ccattaaggc aatctggtgc gtacccatgt
     7801 actccaatcc gggcggcgtg gtgtacagcg atgcagtagt aaagcgtttt gcagccctga
     7861 agccgaaggc aaaggacttc cgtatcttct gggacaacgc gtactgcgta caccatctgg
     7921 tagacaatcc gccggtacag gcaaacctgc tggaagaagc caagaaggca ggcaatgagg
     7981 acatctgcta tatgttcgct tctacctcca agatcacaca ccccggctcc ggcattgcag
     8041 tcatcgcagc aagccagaac aacctggcat acctgaaaaa ggctctgggc ttctccacga
     8101 tcggctacga taagatgaac cagctgcggc acctgcggtt cttcggcggc aagttcgaga
     8161 atctggtaga gcacatgaag aagcacaagg ctctgatcgc tccgaaattc cagatcgtcg
     8221 aaagcacgct ggaaaaggaa ctggcagatc tgggcattgc caactggtcc aacccgcagg
     8281 gcggctactt catttccttc aaccagaagg gctgtgcaaa acgcatcgtt tccctgtgca
     8341 aggaagccgg cgtggttctg accggtgccg gtgcatcttt cccccacggt gtggatccgg
     8401 aagatgaaaa tatccgcatc tcccccacat tccccacaga ggaagaactc cagaaagcaa
     8461 tggacgtttt cgtttgtgct gcaaagctgg ctgccgcaga acaggctctg gcaaagtaag
     8521 cacttcgctc agcatataaa accaaaaaat ttccggacgg acagggacaa tctcctgtcc
     8581 gtcttttttg acaattacca caaaagtcta ttgccggttt tgtgcagtat ttttgccctg
     8641 tccggaaaag cccataaaaa aatacgattt cggggaaaaa tatacgattt tcccgaaaac
     8701 agattgacat ttcgattaca aatatggtac aatgggatta catgtagatg ggtacgggca
     8761 caggcagaca ctccggtgta ccggaaagtc gcatggaata caaaacattc tatttttatg
     8821 caggaggtac tccatgaaat tttctgacgg gctctggctc aaccagagag gatttgacgt
     8881 ttcctatgca gtacaggcat acgatgtcac aaccaccaaa aacagcatca aaatttttgc
     8941 cacctccagt gcgatctgga accgtgcaat gacactgggc ggtgttgcgt tcgagatcac
     9001 atacaccgca gttgcaccgg acatgatccg tgtgcacatc tgccaccata agggcagcct
     9061 gcaaaacaag ccccagtttg aactgaacct gccggagggc tatgtgccgg acgacattca
     9121 cgaagaagac ggctttgttt ccatgactgc cggtcacacg actttgaaga tcaagaaggg
     9181 cacagacggc tgggacgttt cgttctcccg tgacggcaag cggctcaccg gcggcggctg
     9241 gcgttccact tcctatattc aggaaaacaa gttccaccgc gacgcacgtc tggctgtcca
     9301 gaccgacgac gacttcttca actacccgca ggacgcacac aacactttca tgcgggaaca
     9361 gctgacactg gacgttggcg aatgcgttta cggcttcggc gaaaagttca ccacattcgt
     9421 gaagaacggg cagaacgtgg aaaactggaa ctctgacggc ggcacctgct ccgagcagag
     9481 ctacaagtgc attccgttct acatcacctc caacaagtac ggcgtgctgg tcaacacagc
     9541 ggacaaggtt tcctttgaag tggcttcgga tactatcaac aaggtttcct ttaccgtacc
     9601 gggcgaggaa ctggactact tcttcatcgg cggcgaaaac ttacaggaag ttctggtgaa
     9661 ctacaccaat ctgaccggca agccggctct gccgccggca aagaccttcg gtctgtggct
     9721 gtccacctcc ttcaccacca cctatgacga agaaaccgtt acttcgttca tcgacggcat
     9781 ggcagaacgg gatattccgc tgcaaatgtt ccacttcgac tgcttctgga tgaaggaata
     9841 cgaatggacc aacttcgagt gggatctgga tcagttcccg gatccgccgg caatgctgaa
     9901 gcgtctggca agccgcggtc ttggtctgtg ctgctggatc aacccgtata tctcccagaa
     9961 gtccaagctg tttgacatcg gcatggaaaa gggctacttc atcaagaatc cggacggcag
    10021 cgtcttccag accgacctgt ggcagccggg catggcgatc gtagacttca ccaatccgga
    10081 agctgccaag tggttcgctg ccggcgtgcg gaagctgtgc gaaatgggtg tcaccgcgat
    10141 caagacggac ttcggcgaga gaatccccac caaggcagtt tactacaacg gtgcagatcc
    10201 gatccacatg cacaactact acacctatct gtacaacaag accgttttcg aggtactgga
    10261 agaatactac ggcaagggac aggcatgcct gtttgccaga agtgccaccg tcggcggtca
    10321 gaagttccct gtacactggg gcggtgactg ctccgcagag tacacctcta tggcagaaac
    10381 cctccgcggc ggtctgtccc tgtgctcctc cggcttcggc ttcttcagcc acgacatcgg
    10441 cggctttgaa gcaacagctt ctccggacgt gtacaagcgt tggtgtgcat tcggcctgat
    10501 gtccacccac agccgtctgc acggctccac ctcttaccgt gtgccgtgga acttcgacga
    10561 ggagtcctgc gacgtgctgc ggttcttcac caagctgaag ggcaagctga tgccgtatct
    10621 gttcgcacag gcagtcaaga cccacgccac cggtatcccg atgatgcgtc cgatggtcat
    10681 ggactacacc gacgatattg cctgccgata tctggatcag cagtacatgc tgggtgacag
    10741 cctgctgtgt gctccggtat tccgcgagga cggcgtggca aacttctacc tgccggaggg
    10801 caagtggtat gacatcatca ccggaaagac ctatgagggc ggcaagtatc acgctgtaac
    10861 ctgcaccttt atggaaatgc cggttctggc gaagccgaac tcgatcgtca ccttcggtgc
    10921 attcgagaac cagttcgaat acgactatct ggaaggtgca gatgcaatga tctgtaatct
    10981 ggaagacggc aagaccgcag aagcttccat ttacgatacc aaggcaaatc tggtgctgac
    11041 cattcaggcg acccgcaagg gcaatgtcat ctccgtggaa accagcagca ccgacaagac
    11101 ctttaccgtt tctgtggccg gcaccgacaa gaagatcacc ctgcagggcg gcaagggcga
    11161 gatcgtactg taaaaatcag catcaaccgg aaaggcggca gtcatgacaa aaggaaaaac
    11221 caaagacggg ctggagtatc tctggacgga caaaaagcgt accctgttcg gactgcccct
    11281 gtcctttacc cgatattatc tgacagaaag caggctgatc acccgcattg gcctgctcac
    11341 cgtagtggaa gacgaactgg acatctaccg catcaccgac aagaaaatgg aattgccgct
    11401 gggacagcgg ctgttcggct gcggcacgat cattctgttc agcaaagacg ttgacactcc
    11461 ggaaaagcac attcactcca tcaagaatcc ccgacaggtt tccgacctca tcacggctct
    11521 catcaacgaa cagcgtgacc agttcgggat ccgcggacgg gacatggtgg gaccgtccgg
    11581 catgccgccg gaggcatggg agaatgacga ctgctgcaat gactaaaaca ggcactacat
    11641 gcagcagcca aacccattca tgcgaaaaag agaaaaggag gtcctgaaca cattgctttc
    11701 tttcatcaca gaaaaacagt cctcgtggga aaaactacag gcggaaaccc gcccgatctt
    11761 tatctacggc atgggcgacg gtgcagaaaa gatcatgcgt gtctttcggg aaaagtccat
    11821 tccgcttgcc ggcatttttg caagcgatga atttgtacgc gggcattcct ttgccggcta
    11881 caaggtgcgg aagctctccg agatcgaagc acaggtagac gactttgtga tcgtgctggc
    11941 gtttgccgca gggtatcagt cccttgtgga caagatcgtg gagctgaaca gccggcacac
    12001 cctcattgta ccggacgtac cggtagcagg gggcggactg ttcacctatg attactgcgt
    12061 agagcacgcg gcggaactgg aagaagtcta tgagatgctg gcggacgacg aatcccgacg
    12121 ggtatatgcc aacatcatca atttccgcat cagcggcaat atccgctatc tgatggacgt
    12181 gacttcgccc aaggcggaga tctaccgcaa gatcatcaag ctgacaccca acgagatcta
    12241 cgtggatcta ggggcataca acggggacac cattgacgag gtgctccaat acacccgcgg
    12301 aaagtatatc cgcatctacg ccgtagagcc cgaccggaaa aacttcaaga agctgtgcaa
    12361 gtatctggac ggcaagaact ttgtctatgc ctacaatgcg gtggcatggt gtctggacac
    12421 cacggtgccc ttttccacca aagccggcag acagtcggca gtttccgcct ttgggcagga
    12481 gatcgacgca cgtgctgtgg acagcatctt acaaggcaga ccggcgactg tgatcaaaat
    12541 ggacgtggag ggctgtgaac gggaagcact ctggggagcc tcccagacga tcgcacactt
    12601 ttctccgaag ctgatggttt cgctctatca ccggaacgag gacatctttg ctctgccgct
    12661 gctgatccgg cggctgaacc cgaactacga cttatatatc cggcaccagc tgtatattcc
    12721 cgcgtgggaa accaatttat acgctgtgcc ctcacaaaag aattcgctgg cataaagccg
    12781 gcagaaaaca aaactccctg cacgaaaacg tgcagggagt tttgttattg agctcataca
    12841 tcgtagggtt gtgctgtgtc gcagttcttt gtgtaatctt cgtgcaacgc attgccatac
    12901 agtacaaggg taggcgagtt cttttctttt tttgcttctt tttttctttt cagtaaagaa
    12961 aaaaagaagc gtaactcctt gtgaaatcgg ttgtacatga aagataataa acagataaac
    13021 ttgcaaacgc cgtatgaaat aaagttaccc acctgcacaa gaaaacactt gaatatcaac
    13081 attaccgata caggcaaggg caacaaccgg cgttggcaag ccccaggcag acagcgaggt
    13141 acgagcgaat tccgccggac ggtttcgaca tggctgcact agaagttacg ctttgcggag
    13201 gaacccctcc accgcctaac ggcggttccc ctcccctttc aggggaggct
//
LOCUS       DAWBGR010000059        13126 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1471_length_13126_cov_3.474562, whole genome shotgun sequence.
ACCESSION   DAWBGR010000059 DAWBGR010000000
VERSION     DAWBGR010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13126)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13126)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13126
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1471_length_13126_cov_3.474562"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    439..448
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2144..2153
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5330..5339
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8485..8494
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11003..11012
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3775 a         2758 c         2615 g         3928 t
ORIGIN      
        1 ttgtagcaat aaacaaaatt ggaatcaaat atttgtctat tttgctaaat atagccattc
       61 tagtataatt ttagcataaa atactttttt cacacactta atgtatcata aaaacaaaaa
      121 aatcaatact tttgcactaa atggtcgttt ttcagcattg tttggtcaat tttcaccata
      181 ttttaccata ataattctat ttaagatcta tttcaagaag acgtaaaaac gtgtgggttc
      241 ttctttgtca atatgtcaat ggctctctat aacatataat atataatcct aaaaacagtc
      301 aaaaaagtta cggtttttaa aaaaattcta tcaattatgt attaatgcac aaaatcgaaa
      361 tcaagtattt ggttgttttg aaaacaatta gctgtaaata aaattatagc ataaaatgtc
      421 aaaattggta tgaatcagnn nnnnnnnnaa aaaatcagaa aaatcaacca ttttgcacta
      481 aatggtcatt tttttacgtt aaatggtcaa tttcaactat tttctaatat atattcatcg
      541 attaacctca aatattcctt ttcctccgac tcaatgacat ccatcgcaaa ctgttctgca
      601 tcgcaaacag aatttttcag tatctcaaat cctaccgcag cagtaaacac atccacggtc
      661 acttcttccg attggttgga gaaaatttca atctgaaatc cgttgcaatc actgatactc
      721 ccgtcttttc tctccctctg aatatttttc aagggcgtaa tttttacata aaatccatga
      781 tacagcatca aaatatctcc attccgaatt tagattcaaa ctccatattt tccgctagaa
      841 ccgcttcttc aaacgcagtt tccagatatc ttgtgtccag tccgttttcc ttgtagcctt
      901 gctttattat gccgtagtac tgctctgtgg gagcttcaag cggcgtatct ccgttcatga
      961 tgtaaaccat cgcttcaaca gtttctccgt taagttcaag agaaatattc tccttgcgat
     1021 aatacttcgg ctcgccttcg taacgatcta aaaatttttc atcctgcgaa tcaagctccc
     1081 ataaaagcac aggaacttca ctttcctcat tcggttcaat cgtggcatga tgcctgaact
     1141 gcaactcata gccctttagc atggctgttc ctgcaactgt tgaatgcggg cacctatatg
     1201 ccatttgttc caaattgatg ttgctgccgt aagctgcata taattttttc gccataaaaa
     1261 gctcctttac attgatatgg aaaagtccat actttcctca aattcttctt gctcgtaatc
     1321 ttcctcaaaa tcgtccacag cggcttgctc cacttcatcg ggtaaactct cacattgctc
     1381 cagaacttcg cttacaggat gctcatgagc tgcgatacgc tcttctttca gccgttctcg
     1441 ctgagcaatt gcatcttcgg gatgccgcca tgaaatacat ccgtccagat gcgaaatcag
     1501 gtgcttgcgg caattgcgga attcctcacc gttcagtcca agattaatca gccaaaccct
     1561 tgcactgtac ctcagattat cgctctctga aatgtgcggc gaacagaatt ttttcgtcag
     1621 cgcagcattg gaaatggcaa gcgcaagagt cacataagat ctaataactc cggcgtgcaa
     1681 tgaactattg caggctctca tcttccaatg cccattagca aaaaaactat gtaaattaca
     1741 agctttatag cgagtggaac tgtaatgatg atgcacctca tccatatcgc ctctatacca
     1801 caatttctgg attgcttcga tagttttagg tttctctttg ttcagcattt ccagaaaatc
     1861 tctgtccacc ttctggcagt aagtttctct cataggcgat acctgaagca tatcaaacaa
     1921 gaagttttcc ttgctggcaa aaatgttcac caagtttcgc aaactctgcg ccgtataagg
     1981 tgcaccgtct acatgaatgt gaataccgca attgtactgt gctccggtca caccgtcagc
     2041 gcttctgacc gcccggacaa tttcctgcaa cagtggaata tcgctatatt ccagcaccgg
     2101 tgtgacgatt tccacggcat actctcttga cgcattaccg ccgnnnnnnn nnngtttttg
     2161 tccacacgcc gaatgctgga atcataaaca actgcccatt ttctgtcctt gctatcacaa
     2221 atatgatact tatcgtaact gccgccaaag tgctccggca cgctgtcaaa tactctgccg
     2281 attgcccttg cggctgcgtt tctcgtcaat ccggtacact caatttccac gccaaatttt
     2341 tgcgttttaa tcccgtcaaa atttcctgac aaattctcat ctcccatgaa aaaacgacct
     2401 gcaatgcaag ccgtttttct tgatatttcg ttatttttcc atctgtgctt ttacttccac
     2461 gccgcccttg aaaacgatca gtaatttctc tttcgacagc actttcacgc aatcaatcag
     2521 cttgcggaca atcacattgt caaacacttc aacctgaaag ttttcatgtg ccagcatttc
     2581 catggcagaa tcgattttat tctgcgtatc cgttgaaatc tctgcattct ttcggagttc
     2641 cgaaagcttt tcatttatct cttgttcctc tgcatgaatt ttttcaaact cgcagtccag
     2701 cttattctca tcacatccgc cgccagtaat cagcgatatc aagtcgtttc gggctttatc
     2761 taaatccttc agccgctgtt ccagattcaa aatttcctgt tggtttttgc actctagaac
     2821 cgagccgata tttgctttca aaattttagc aatctcatct ccacagccga gatattcgtt
     2881 cactgctttc agaatcgctc tatgcaactt gtcctcatga atggtcggcg attcggaaca
     2941 atactttttc ccatgatcca gacggctgat acatcgccag acaatctgct ttttaccgcc
     3001 agccgtccat gtacaccgac gatatggcgt gccgcaattg ccacatatca aaagttcgga
     3061 aagcgcgtat ttactggaat attttccttg ctcggtagtc gttttatcgc tgattttacg
     3121 cttactggaa cgccttgcaa gttcttgttg aacacggttg aacgtgtccc tatcaatgat
     3181 tggcgcatga tgttttgtca ccagatacat gggacgttca ccgttatttt tcacaacctt
     3241 tttggtaatg cagtcagcag taaatgtttt ttgcagaagc acatcgccca catatttttc
     3301 atttttcaga atgcttttga tgttggattc gctccagatc tgattgccca tggcagtcag
     3361 tttcccttta gattcaagag cattctttat ccttgtgaag ctcaatccgt ccagaaataa
     3421 ttcgtatatc aaatggactg tttccgcttc atcaggaaca atttccggct tgccatctgc
     3481 acctttctga tatcccagaa gcttggaata ttgaaacgcc accttgcctt ctgcataagc
     3541 tttttgcttg ccccagctga cattcttact gatggactca ctctccgcct gagcaaatga
     3601 gccgtacaga gcaatcataa attcacttgt catggtcatt gtgttgatgt tttccttttc
     3661 aaacaccacc ccatcgctat cataacgtat tgtatcgttg aaaagtgccg agtttacgcc
     3721 aaattcgtat gtccaaaaat gtgtatcgct gcatatcgta tcccctcatt ttgtgggttc
     3781 aaacaccaaa taaacgccat gctgaacgga tttctgtgtc cgaatcagca tggcgtttgt
     3841 tcatatattc aattgactgt tccaagtgcc tgactaatgg catcggaagc tttcgctctt
     3901 gtttctgcaa aaatatgagc gtagatattt tccgttgtgc gagtatcaca atgtcctaaa
     3961 tgttccgaaa tcaccttaac cggaactccc atattgataa ggagtgaagc gtttgcatga
     4021 cgaaggtcat gaatatgaat attaggaaaa tcatgttttt tcagcagttc tttaaatgtt
     4081 gtatttacat aagtggaatt catgtactct ccgtactcgc ctgtgaaaac cgtacccctg
     4141 tcaatccatc gatttccgat atcctgcttt ctatgctcct gccattccat ctgctctgcc
     4201 agtgtttcca taacctgtgg cggtagtgaa attactcttg cactgctttt cgttttcgga
     4261 gtaccaagct tgtactgccc tttgctacga taaagactat atttgactgt cagcgtggaa
     4321 ttttcaaaat ccacatctgc ccagtgcagt cctgtgattt cacctactcg cattcctgtg
     4381 aacagcagca ctctgataag ccttgtaagc tgtggattac tgaattcatt gatgatagtc
     4441 agaagttctt tgcattgttc tgcattcaga tacgctcgtt cctttgtatc tgtgtccttt
     4501 ggtgtggtgg cattcataac tggatttttc agcaacaatt ctttcttgac agccgttgaa
     4561 aaaatcggag aaagtgcagt gcgtaccctt gcaattgtgc cgtcctccaa accgccacta
     4621 ctttccgcaa gtgtgaatgt ctgtttcagc gtcttgccac aagcctttga aactttttct
     4681 gctgtagaac gcatcacagc cttaccattc gccatatccc gcagcgtatt catatttact
     4741 ccagattttc gggaaagcgg tctgcgatag ccgtgagcaa gcaaagaagt atcctttaaa
     4801 atgtacattt cacgcttact gccgtggcga tgcagttcat tgaacagtgc gtcaattctt
     4861 gctgtgttga tgtctttcag cttcatgttt ccgatgtatg gcagcacata agtttcgaga
     4921 atataacggc tggattccag tgtatattct ttcagcttat gaggtgcaat ctgatcgtag
     4981 taccagtcaa ataactcatg aaagcgaata ttttcatcaa gattcgtcat gcccctgcat
     5041 cgctgctcaa attcataggc atatgctctc gccagttttt ctgccttacc ctcagttact
     5101 ccgtcaggcg gtgtaaatgt ggtggtcttg cgtatctgtt tccccgccat atcatagccc
     5161 cttgacacca tgatacgaaa agattttcct cgtttcttaa tacttggcat tttgtagcct
     5221 cctttcagat gttgccttac tctatattcg ctctgaatcg gctgaaagtc aagtaatatt
     5281 gtggattatc cggtagaaat atcactgaga aatttgtgca gattattctn nnnnnnnnnt
     5341 tattcttcgg aaacagtact gtcctcaatt tgtaagccac gcttctgcaa ctgtttcaga
     5401 ataaattctt ccacaaactt ctgcaaatcc cagatgaaat atccttctat attaaaattg
     5461 cccactttac ttttatcgaa tttcccattt aacacatttc tgacctgtgt ggatttgaac
     5521 tccagttcct ccaaaaatgc gtagaaatcc ttgtcactaa gcagtgaatc caaaagtttg
     5581 atcagtggtg cggtactttc aaagccctct gtattttttg catatttcat gagcgtgatt
     5641 gcactctctg ataagcccaa ttcctcatgc agagccttat tttccgagct gatacctgtg
     5701 agcagatagt caacagatac atgaaaatac tctgcaattc ggatcaggtt ctccggtgtc
     5761 ggctgtgttg ttccattttt atacagactg atggtttgct gacggatgcc gacatattcg
     5821 cctaaagcct tgagtgtggt tttctcgcct gtttttggat ggcattcgag caattctctc
     5881 aatatcgttg ggaacgttcc ctcatatccg gttacttgtt cagccatatg attttctcct
     5941 ttgattttcg atttacttga acttctctaa attgatttcg ctttcattga cattctgttt
     6001 tgcttgtgat ataattatta tatcagatga aacgaaactt gtcaagatga aacggaggaa
     6061 ttttacaatg aaagaaatgc agaaacaacc gacaatgatg actatccgtg aaattgccgg
     6121 aacagggctg atgtctgaac acgctctccg aataatgctg aaagcaggaa aattaccggc
     6181 aattttcatt ggtaaaaaag cacttatcaa ttatgataag ctgtgtgagg aattgcaggc
     6241 attagaggcg aatgtggcaa agcaggaaga gaacgatttt tggaattaat catattgttc
     6301 aagatgaaag gaggggtgta ttttgctcaa gttgaaacaa atgaagatct ggctttgctg
     6361 gaattatatt gaagttaagg gaaagaaaac aaaaaaacct gttgcagccg gaggcggtgc
     6421 gacaggtaca aacagcgagt atcaaaaaac atgggtaaca tatgatgaag ccatattggc
     6481 aaagcaacac atgaaagctg atggcattgg ctttgtgatt ccggaggggt atttcttcct
     6541 tgatgtcgac catcaggaac tgacaaatcc attcgtacag atgatgctga aacgcttcag
     6601 ttcctatgca gagatttctc cgagcggaaa tggattacat atctatggta ttgctgatat
     6661 gacaaggctg cctatggcac aagataaaga cggcaaacag aaacttgaca gacaattcta
     6721 tatgaaaaat ccgaacaatg ggattgaatt gtatttcggt ggtctgacaa accgctttgc
     6781 aacatttaca ggcaatgttg tgcatgatgt cccactttgt gattgcaccg aagctgtact
     6841 gacaacactt gataaagata tgcgaagaag tcaaccgaga aattacagtg ccaagcgtga
     6901 tggtgacagg gaaacttttg atatcatctg caacctccgt aaacagaaga atggtgcaaa
     6961 gtttatcaag ctatttgata aaggagattt cagcgattac ggcagtcagt cagaagctga
     7021 ccttgctctc tgcacgatgc ttgcttttcg tgcaggaaat catcctgaaa tgattgatac
     7081 gctattccgt cagtctgctc tgtatcgtca gaaatgggac agagctgatt atcgtgaaat
     7141 gacgatacaa agagcaatag aagcttgtgg agaacaattt cataagagtg tcatgccaca
     7201 tcctgattgg atatcctata gcgaaaaggg gattcctcgt atctctgcga caaagcttgc
     7261 aaaggcagta aaaacaagag tgaaatatat tctcgtccgt gacaatggca aagaaggtgt
     7321 gcgtgtatat gtctatgagc aaggcgttta tgtgtattac gctcccgata tgttcaaagc
     7381 cgtgataaaa aatatcattg caaaatacga tgaagatgtt gtcagtatgg gggcagtaca
     7441 tgaaacatac gcaattctga caacagacct cgactatata tctgaatccg cattgaatgc
     7501 ccgtgaggac atcatcaatt ttcagaatgg gttgcttgtt gtgactgcca cagatgttca
     7561 tttgattcca tatacccccg atgttctaac aaccattcag attccctgca actatacaga
     7621 ggagttgatc cccacaccga atttcgaccg ctatcttcat acgctgacaa acggcgatcg
     7681 tgatgtacag cagcttttac tggaatttat gggcgtggtt ctctccaata tcaagggtta
     7741 cagaatgaag aaatctctgt tccttgttgg tgcaggtgat acaggtaaat ccgttctgaa
     7801 atctttaacg gagcgtctga ttggcaaagg gaactttatc ggtattgacc tcaaagaaat
     7861 cgaagcccgt tttggtacgg gtgcgattta tggaacaaga cttgcaggca gttccgatat
     7921 gagttttctc tctgtagatg aactgaaaac attcaagaaa ataactggcg gtgacagtct
     7981 gtttgcagaa ttcaaggggc agcaggcatt tgaatttacc tatgatggtt tgctatggtt
     8041 ctgtatgaat cgtctgccga agttcggcgg tgatgatggt aaatgggtat atgacagaat
     8101 tatggtggtg gaatgcaaaa atgtaatacc tcctgaaaaa caggacaagc aactcctgga
     8161 taaactgtat gcagagcgtg agggaattgt ccgcaaagca gttctggcaa ttcaaaacgt
     8221 tattcgtaat ggttaccgtt ttttcgagcc aaagtctgta accgttgcaa gggagcagta
     8281 tatgagtgat aataatacag tcgtcagttt ctggaatgag tgcatggtgc agagaaacaa
     8341 gatttctgat aaagccacag ttggtaaaat ttatgatgtc tacaaggcgt ggtgtcagga
     8401 taacaacagt ggctatgcca aatcagcaaa ggagttcaga aacatcattt ccgagtattt
     8461 aaatacgtct tattcaaatc tgacnnnnnn nnnncaggaa tcatataccg aaactataca
     8521 ctttctgtag ataccaaaca gcagtattgc agaatctatg ggtttgatga aattctgtgg
     8581 tgaaagccta caccaactac acccaactta caccataact acaccttgta aaatctcgta
     8641 ttttagccac aattctgctc tttggtgtaa ttggtgtagg ttctggcaac ttcttagatt
     8701 ctgaaaaaaa ctttgaacct tgtatctgac ataggtacaa agtacaaggt ttattttagg
     8761 aaaagagatt tcaggaaaaa ctacaccaac tacaccaaaa acactgaaac cacgcagaat
     8821 agcagaaaag tgtggtgtag gtttgatgta ggtaaggtgt aattagtgca ggtatgaaag
     8881 ggtgtggtta aaatgagcag tttatcatcc gaatatccac caaagagaaa aggaggtgca
     8941 gtatgttatt aaaaccgtcc gatttatcta atgctgccta tgactgtcac attatcatgg
     9001 aaacattgaa aggagaacct gtactgatat taaagaaaaa gaccgattct ccgatgaatt
     9061 ggtgcgttat acagaggttc tcaacctgtt tctttgctac cagaaaagaa gcgatagact
     9121 tctgcgaaaa gcatggttgc agcattccta agccaccccg agggggcata aaaatcgcta
     9181 aaaacgattc atctgctgac cgtctggcag cagtacaaac aaaaacacat attcaaacat
     9241 ggtattaacc ctgttatgaa ccgaaattgt ctaaaagtaa actgaaaata acaaaaggag
     9301 aaatcacgct atgttactga taaaagatta ttacagagtt gcatttgaaa atcgcctgag
     9361 aaccaatcaa ctcggtgatg ttgacgatta caatctcaaa cttgcaaagc tgaaaaacaa
     9421 cagctatcca ttaccgtata gtttcaaggg agattataca gaacatcttg ctcatgaaaa
     9481 tttgttccgc cgtttcggaa cagtgattca cacggaaatt gaagaaggaa ctgtggtatg
     9541 tatggatgtt atgccggaac ctgaaatcat tgatgagaac cagtcttatc cctctccgca
     9601 tgaaatgcgt tatccatgtc tccgctgccg ttcacacaaa ctggcaatgt taggaaagat
     9661 tccatgcagc ttcgttgatg atacacgttt tgaccttgca ggctatgttt acagggaaat
     9721 cggtcactgt ttcggtagag cggaagaaaa tatatgcctg aatggtatcg ggcagactaa
     9781 accaaaggga ttgctccatt ctgctgaaat tggtgttaca gcaagtacac tcacagcaga
     9841 tgcagttatt gaattgttct tcagtctgga caagcagtat cgcaaaaatg cggtatgggt
     9901 gatgaatgac gaaactgcta tgacacttcg tatgctgaaa gacagtgccg gaaattttct
     9961 ctggagaagc acggacgata cgattttcag ccatactgtt gtcatcagca actacattaa
    10021 cgacagcaca attgtcttcg gagacctttc ttattattgg cttattgagc gtcaacccct
    10081 tgctgtacgc cctctaaacg agctttacgc acaggaaagc tgtcttggtt tagctgcttc
    10141 ggaacgcatt gacggcaagc tggtgcgttc agaggcagtc aagctgttta aactgaatta
    10201 aaagcgtttt ttattctatt agtctatatg tctaaacgca gaattacccg taagatttcg
    10261 caaaagaatc ttacgggtaa ttttatattc agtaccgatc ataccaagcc gtagtaaaaa
    10321 acagcgaaag gtgtattttt cattgtcggt tcccttaact gttcccactc ttttctgtct
    10381 gtttgtcatt tcacacagct tggtgacaag ttctgtatag gctctgattt catcagaatc
    10441 ctgcgtataa ggaaaccatg gaaagctgat tttctccaca ctggttttga ctgtaagcgt
    10501 atctgttccg attgcttttt tcagcagact tgctttgcct gcaatgattt tctcaagcat
    10561 tgtcagtttc tcgtccgaaa gtgtatttct cggaacagag attgtaaaac gatctgtgtc
    10621 tgtttctccg ctctcccaac cacgctcttt cagatgattg agaagtgtca tagcatcaat
    10681 attatcggaa atcgtcagcg ttccgtcacg ttccagaatg cagtcgccaa tctgatagtt
    10741 acaggttggg actcccagat aacgtggaat tgttccaagg atttctccga tatggtcggc
    10801 aagttccctg cgtgttctgt tttgcagatt gattttcatt tgaattgtac ctccttgaat
    10861 ttggtaggta cattataaat caggtttggg ataatagcaa tggtgtgtag gagagttata
    10921 aatttattat caaagcaata cacaagatgc atattttatt aaaattaata tataagataa
    10981 acgatatata gggtttcact tgnnnnnnnn nngtataata cagacataga accaacgaat
    11041 ttgaggtgaa ttgcttatga tcgatatgca gccgaagaag atgattatac ttgacatctt
    11101 gcatatttta aagaaacatt ctgataaaga tcacaggtta tcccagcacg atttacaaga
    11161 attattggaa tcggaatatg gaatgaaagt agaccgcaaa actatcagcc gcaatattat
    11221 caaacttgtg caatgcaaat atccaatcga atatggcagc aacagcgatg aatcaatatt
    11281 gacaaatata tattacgatc acaagttcct aaacggcgaa cttcgtctgc tgatagatag
    11341 tgtcctgctg acggacggtc tgtcgaaaaa agatcgtatc agtttgatcc gccgtattga
    11401 gggactgtcg agcaagtatt ttcattctga aatatccaag atcgatatgg atatatacgg
    11461 caggattgaa aacagggaaa tcatactgac acttgaagaa atcggcagtg ccattgctga
    11521 gggaacacag gtcacatttc attattccga ctgtggtatt gatggtgaat cgaatatcag
    11581 gcgtgacagt aatgggaaag aaaagcagta taccgtcaat ccctatcaga tcatatcaaa
    11641 aaacggacat tcttatttaa tctgcaacct gccggaatac catgatctga cacattttcg
    11701 tattgatcgt atcaaggact gccgtaaaac agatgtgctt tccagatcac ttcgtatgct
    11761 gaaaggcttt gaaacaggta tcagactatc ggagtatatc aaatcacatc ccaatctttg
    11821 gagtggagtt cccacacata ttacgttcca gtgtaagcaa tatatgatga acgatgttgc
    11881 agacagcttc ggcactgaac tcaatataga agagttgcct gatgatatga tgaaagtaca
    11941 cgtttatgca agcgaatctg ctatgcttca ctgggcggtg cagtttgctg atgctgttga
    12001 ggtcttatcg cctgcaagtt tacgcaaaca gattgctgaa acactgcgaa acgctcttga
    12061 aaaatatgaa acataagtat cccttttttg gtacatcgac cgtgatataa tagaattaca
    12121 aacggaaggg aacgttcagc tgataccgtc cgttggtcgt gttgcacatt tacgacatca
    12181 ttcttttgtg caaaagggag aattttcgga ttttcacccg aatcttcgga attgagattt
    12241 ttgtgtgcta taatggaaac ataaagagaa agagtgagga ggtatgctta tgtatcagta
    12301 cacttgcgat tcaaaatatt gtgccacctg ttcctgctgg tctggcgaac ggaaaatctg
    12361 cgatcatttt ggctcaagac ttgaggtcag cagtccaatg acaacaggca agtgcatgaa
    12421 tcagaagtca ggtgcgtggc agcaaacaag acaggcaaac ggcggatgct ctgcacatga
    12481 gaagtgggga gcgttgaggt aaaaacattt tcagtcaaat tatgctatct attaaggaga
    12541 aaatgcttat gttagtaaca tccgcacagg cagcaaaatt gctgcgtcag ttcaacgatg
    12601 aactgagagc attacagctc aaagaggcaa actcctgcag ctttgtagcc gcaattcagg
    12661 aggatgtcga atctgtacgt cctgagtaca atttcaagga aatgcgtgat gctcaggcag
    12721 aggttgagtg taagatccgc aaagtaaaac acgcaataaa cgtgttcaac acaacaacga
    12781 ttattccgga ttttaatatt acgatcgacg agatgcttgt atatcttcct cagcttaatg
    12841 cgaaatgtac gaccctctct aaaatgagag atgcaatgcc gaaagtgcgt gtcagctcag
    12901 gttattccgg cggtggcaat atcatcgact ataaatatgc taattacgac atcgaagaag
    12961 tcagcaagta ttatgccgaa ctttccgata cgcttgcaaa ggctcagaca gtgcttgatc
    13021 tggttaacag cactgtcgaa ttcgagataa atatctaaga cttattccgt ttacagttct
    13081 cacgcttcct tatgaatatg actttgttcg tgttcaaggt tatcgt
//
LOCUS       DAWBGR010000060        12898 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1493_length_12898_cov_5.491630, whole genome shotgun sequence.
ACCESSION   DAWBGR010000060 DAWBGR010000000
VERSION     DAWBGR010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12898)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1493_length_12898_cov_5.491630"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3050 a         3457 c         3494 g         2897 t
ORIGIN      
        1 gaaaaatttg acgacgcatc tgtcgcacaa tctttgtgcg gtattgcctt gacaacattt
       61 ggcgaagcat atcaaaaatg gtaatgacag atgacttttt catgcttttg cgttctccga
      121 ttccccgccg ccgaccccgg tattgcctaa actgtttgca caatttgagc gttctggatt
      181 ccgtcacaaa aaatttttct gtttctgttt gcaggcatgt gcaggatctg tggcgaaatg
      241 tcgaaaaaac cacgcaattt cgccgctttt gacgattttc acaaatcggt caaaaaaatc
      301 gccccaattt tcaccagctt gacattttca tcggaaattg ctataatatt taagaactgt
      361 tttgcgggat ttctgtataa tttgtagaat tcccacggtg gtgcaccgcc gatcatgcgg
      421 tcatcagcag aaaaaacggc tgtctgtccg atttgcacag atagtcaagg tggcattata
      481 tgcaaagtgt ggtgctgccg aaaaaatacg tccgtccccc aaaagccacg gcggacagaa
      541 agaagggtgg aacagccttg gcgaattaca aatggcacaa acgtgcagcc gccgcagtac
      601 tggcttgtct gatgacagcg gcggcacttc ctgcgacggc ggcatttgcg gcaaagaccg
      661 acacggtgca gaccacagcg gcatctgcgg agaaggcggt atcggatacc ggcggcgacc
      721 tcacctatga ggagcaggaa acctacagcg attactacga ccagtatgcg gagcagcctc
      781 gtccggcggc ggagatcacc gtttccggtg cggattttca aaaggcagac tgcgaaagct
      841 atgaagcagg cagctatact gacaaaaacg gagaatctcg gaacaatgcc ctgatctgga
      901 acaatgcgga gggcagcttc agctaccagc tgaacgtgcc ggaaaccggc atctattccg
      961 tgcagataac ctattgtccc atcgtttcca atacctcgga gatctccgtt tctctggcaa
     1021 ttgacggcga ggtgccctat gacaccgctt ctcgtctgaa actgaacaag gtctaccaga
     1081 acaagaccga gatcaagacc gatgacgtgg gcaatcaggt acgtccggca caggaacaga
     1141 tcgaattgtg gcagaccaca tggctgggcg actccgacgg gctgttcaat gagccggtgt
     1201 tcttctatct ggaaaagggc acgcacgaga tcaccctttc ttccgaaaag gcgtattttg
     1261 cactggaaac cattcgcttt gcacagccgg aagaagtttc cgactatgac acctatgtca
     1321 gcagcgtcaa tgcgtccgtt tccaaggaca gtacgccctc tggtgtggtt cgcatcgagg
     1381 gcgaaaacgc cgtgctgaaa tcggattccg tgctgtatcc gacctatgac aattcctcca
     1441 gttccatttc cccttccgac ccgaagcaca tgctgtataa caccatcggc agcggcaact
     1501 gggaaaaggc actccagacc attacatggc aggtagatgc aggcaccctc gccggcgacg
     1561 gctggtacaa gctgggtatc aaggcacggc aggaggaaat gcgtggattt tactccaacc
     1621 gccgcatcta tattgacggc aaggttccca gcgaggaatt cgatcaggtg aaattttatt
     1681 atgacaccga tttccgcatg actaccgtcc agaacgacga cggcgaggac gtgtatgtgt
     1741 acctcactgc cggcgaagac cacaccatta ccatggaagt aattcccggc gagatcggcg
     1801 actccatgcg gcagctggac gcgatcgtgc tggacttgaa tacgtattac cgaaagattg
     1861 taatgatcac cggtccggag ccggacaagt acaccgacta ttatgtacat gaaaagattc
     1921 cggaactggt ggacgaattc cagcggatct ctgacgaact gaaagccatt cagggacata
     1981 tcgagtcgct ggcaaactcc aagggttctg aagccgcttc tctggaacag atgacagtca
     2041 ttctggacaa gtgcatcagc gaacctttga agatcccgaa ttacctgtcc cagatcaagg
     2101 actatatcac ttccctgtcc tcgtggatgc gggactaccg cgaccagccg ctggaagtgg
     2161 actatctgga actggcttct ccggacgcag acttcccctc tgccaaggca ggcttctgga
     2221 gtgcaatctc ctatagcttc cagcgtttca ccgcatcgtg ggacgaggat tacagctcgc
     2281 tgtcctccac caccggcgat gatgccattg aagtatgggt ttcgctggga cgcgatcagg
     2341 cacaggtggt caaagatctg gtggaatccg aattccagca gcagtacaac gtgccgatct
     2401 cgatcaatct ggtagtcggc ggtgtcgtag aagccacact ggcggacaag gggccggacg
     2461 tggcactgtt cctcggcggc gaattccctg tcaatctggc agcccgcgga ctgctggcag
     2521 acgtttcccg gatggacggc tacgacgaaa tgaccaggct gtatcaggat acggcaatgg
     2581 tgccctatca gtacgaaggc ggcacctacg gcctgccgct gacacgcagc tgggcaatga
     2641 tgttctaccg taaggacgta ctcagcgaac tgggcttcac acaggcaccg cagacgtggg
     2701 aggaactgat cgatatgctt cctgccctcc agcggagtta catgtctgcc ggtctggtgc
     2761 tgccggcggt agcggcagat gccaatcagg caaccatttc tgcggcgacc gaatccggtc
     2821 tgacctttgc ttcgctcctg ctccagcgtg gacagaacta ttataatagt gcacagacaa
     2881 agaccacatt cgacaccaac gcggcgatcg atgcattcga gatgtggaca gacttctata
     2941 ccaagtacga cttcgaccag cagtatgatg cattcagccg gttccggacg ggagaatatc
     3001 ccgttgtggt ttccgactac tgcaccttct tcaaccagct ggcagtggcg gctccggaaa
     3061 tcgacgggct gtggggcttc gctcccattc cgggcacttt gcaggaggac ggcaccattt
     3121 ctcacgccgt caactccaac aacaccggtg cagtcatctt caataaggta gacaaaaaga
     3181 ccaacggcat ggacaatgca tggacgttcc tcaagtggtt ctgttccacc gaagtacagg
     3241 tggaatatgg tacccagatc gagggactac tgggacaaat gggacggtac gcaacggcga
     3301 ataccgaggc actcacccag ctggcatggt ccacggacga ggcgaacacc attctgggag
     3361 ccatggacga actggaagag atcccgatca ttccggcatc gtactctgtc acccgaaacg
     3421 tgatgaacgc attccgtgac gtggtcaaca ataacgagaa cccccgtgat acgctgatgt
     3481 cgtacaatcg ggacatcaac gaggagatca ccagaaagcg tgaaaacctc ggattagact
     3541 cttaaagaga aggagggaat ccctttggat actgccgaga ttggcaaaag aacccgacag
     3601 gaaaaccggc ggctcacgtg gctggcggtg aagcagaaca aggcaagcta tctcatgatc
     3661 gctccgttca tgctgttttt cttggtattt accatcgtgc cggtgctgat gtccctgcct
     3721 atgggcttta cggacttcaa catggtacag atgccgaaat tcataggact gtccaactac
     3781 accaccctgt tcctcgcgga caaggtcttt atccagtcca ttcgtgttac gctggtgttt
     3841 gccctgttca caggaccgat cagctatgtg ctgtgcttcc tgctggcgtg gctcatcaat
     3901 gagctgcacc cgaagctccg cacgatcttc accctgatct tctacgctcc gtctatggcg
     3961 aacgtgtaca ccgtctggaa gctgatcttc agcggcgact cctacggcta tctgaactcg
     4021 ttcctgctgg atctgggact aatcaatgca ccgatccagt ggctgacgga cggcagctat
     4081 gtgctgggtg tcaccatcgt tgtccagatg tggatcagtc tgggtgccgg atttctggca
     4141 ctccgtgccg gcttccagaa catcgaccgt tcccagtatg aagccggtgc cattgagggc
     4201 atcaagagcc gctggcagga actgatctat atcacgatcc cgtccatggg accgcagctg
     4261 atgttcgcag cagtcatgca gatcgtcagc tccttcaccg ccggcacggt atcacagaat
     4321 ctggtgggct tccccagtac cgattacaag gcacacacca ttatgaacca cgcctatgac
     4381 tacggctgga tccgctatga aatgggctat gcctctgcga tctgtatgat tctgtttgtg
     4441 gtcatgtata ttctgaacaa gctgatcggc aaagtcctca gcaagtatat ggactaaggg
     4501 ggtgcagtat ggcaaaaaaa gaaaaggtat acggcacaca cctgaaggtt tccaccaagc
     4561 gtgccggaca taggatcggc ggcactgtcg gcattttcgt ctttctgctg gtgctggcac
     4621 tgttcatggc actgcccatt tatctggcag tggtcatgtc cctgaagccg gtgcaggaac
     4681 tgttcgtgtt cccgccgaag ctgtacgtca tcgatcccac gctgtccaac tacagtgcca
     4741 tgttccggct ctgttccgac ctgtgggtgc cattctcccg ctatgtattc aacagcgtct
     4801 ttgttaccgt atccgttacc gtgtgtcagg tggtgttctc cgcaatggcg gcgttctgtc
     4861 tggcaaaggt acgcttcccc ggtgccaagg cgatcaacgc gatcatcgtc gttgccctgc
     4921 tgtatcagag taacgtcatc tacatcatgc agtatatggt catggcaaaa atgggcatga
     4981 tcgataccta ctgggcactg atcctgccct ctgtcgctgc ccccatgaac ctgttcctga
     5041 tgcggcagtc catgacgcag gtgcccgatg ccatgatcga agcggcaaag gtagacggag
     5101 cagatatgtt ccgcatctgc tggcaggtgg tcatgccgaa ccagaagccg gcactgatga
     5161 ctgttatcat cttctcgttc caggcggctt ggaacattca gggcggcacg ctggtgttca
     5221 gcgaatccct gaaaacactg cccaccgtgg tacagcaggc agcatctgcc ggtctggcga
     5281 gagccggcgt ggcaatggca gccgccgtgt tcatgctggt gccgccgatc gtcatcttta
     5341 tgctggcaca gcggcaggtc atcgaaacca tggcacattc cggaatcaag gactaaaggg
     5401 agggtgaaga ctttgcagaa aaaggcaaat catctggcgg cggcgtgtct gtccgtgatg
     5461 acgctgacgg cggcgatcgg catgccggca gtttcggcgg aacattacaa cgtctataac
     5521 tacgaccgct gggacgaagc gacaccgtca caggcaggct atgcggcaca gcgtgctgtc
     5581 agcggtctgg atctgggctg tggtgcgttc aataccccct ccgatctgtt tcgggactgg
     5641 gaggatcgct tttacattgc ggacagcggc aacaaccgca tcgttgtcac agacagcggc
     5701 ttttccaagg caaccagaat ctatgacaag ctgaaaaccg cggacggcga aaccacactg
     5761 aaagatcccg agggcatcta tgtgtcccgg gaaacccagt gcatgtacat cgcagacacc
     5821 ggaaattccc gtctgttggt gtgtgatctg gacggcaatg tacaaatgga actgacacgg
     5881 ccggactcca cattatatga aaacgaaacc tttaagcccc agaaagtcgt tgtggacaag
     5941 gcgggcaata tctacatggt gctgaacaac atcaccaacg gctctgccat gttcaacagc
     6001 gacggcgaat ttcagggcta tttcggtgcc aactccgtgg acgctactgc ggaagtggtg
     6061 gcgaactact tttggaacat gatcgccacc gacgaaatgc gggcgaattc ctcccgtaac
     6121 gtggcggcag gcatcaccag ttttgacatc gacgatgagg gcttcatcta caccgtcaca
     6181 cagtcctcct cctcggagac agaccgtgtg aaaaaggtga accctgccgg ctataacctg
     6241 ttctccgtgc tggaggtgac attcggcgac ctggactctg tttatgactc caccgccaac
     6301 gagaactaca ccacccagat ggtggacatc gacattgacg acatgggacg catcaactgt
     6361 ctggacttgg aaaccggacg ggtgttccag tacgacgagg acggcagcct gctgttcatt
     6421 ctgggtacca cagccgatca gatcggcggc ttttccatga aagtttccgc ggtggaaacc
     6481 atgggcaaga acatctatgt ttcggatgcc atgaagaata ccgttaccat tttcaccgaa
     6541 accgagttcg ggggcattgt gcacaatgcc gtggctctgt acaacgccgg ctattatgct
     6601 gaggcactgg agccgtggcg ggaggtgctc aagcgggacg gcaattacca gatggcgtat
     6661 atcggcatct cgtcggcact ctataatgag ggcaactata aagaggcaat gaaatatgcc
     6721 aagctggcac agtcccgcaa tttgtacgat aaggcgtttg agggctatcg ctctgaatgg
     6781 ctgaatcaga acttcacatg gatcattctg gtggtcgtgg tgctgatcgc cgcggcagtg
     6841 ttcttccact tccgcaacaa aaagaagaaa aagaaccagc cgaagaatct gatcgaaatg
     6901 ctgcacgagg gagaggagga gtgatgctgc tatgatggat ctgacacaaa agcaatgggt
     6961 caagcacagt gtgttccacc cgtttgaggg ctttgaggat ctgcgttgga aaaagggcgg
     7021 ttccgtcctg tatgccagca ttgtgatcct gctctggttc gtggcgaaga ttttgcatga
     7081 caatctggtg ggctatcagt ttgccgtcac caacaccaag atgttcagca ttgtcccgta
     7141 tatcgtccag acgattgcgg tatttttggt gttcgtcatc ggcaactggt cgatctgtac
     7201 cctgctggac ggcgagggca cgatcaagaa aatctatatc tacagtgcct attcaatgat
     7261 cccctacgtt gccggactgt atatccggac gctcctgtcc catgtactga ttcaggacga
     7321 agtcatcttt attacctgcg tcacggtaat cagtactgca tggtctgtgc tcctgatgtt
     7381 caacgccatt aaggcggtgc atcagtattc gatcagcaag accattctgg cactgctgct
     7441 cacctttgtg gcaatgctga ttattctgat cctgctggtg ctgctggtgg cactgttcca
     7501 gcaggtatac gtatttgttt cttccgtcta cacggagatc acataccgcg tgagagtata
     7561 gaaaggacgg tggaaccaat atgaagaact ggaaaaaatg ggctgccggc atctgtgctc
     7621 tgagcctttg catgacggca gtttccctgc cggcggcggc ggagggcgag gacgacattg
     7681 ccctcatttc cgacacctcg gaggagatgc cggcggcaga cggtacagcc gatgccgata
     7741 cggcagacga caccgccgaa gaagaagcga cccgttccga atcgcaggag gaaattgcga
     7801 ttacggcgga acaggtcacc cagtacatgc agaagaagaa ttcctgcgac ggcatcactt
     7861 tctactaccg tccggaggac tatgaggata ccatttccga cgaggacgtg gtagatctgc
     7921 tggacgacat cgaactggca ggcattgacg atgccaccgg cgaggtagtc tgcacactgg
     7981 aagaagacag cgacaacagc gacttcgtgg tattcctaag tccggaaagc cgctggctgg
     8041 tgtacatgga tccggaatac agcaaggtca caatggttcg ccagatcgtt tcttcgctgg
     8101 ataacgaact gctgttccgt tcccgcgaca accggacact ggaactgtac aacaaggact
     8161 acgacgaagt agaacgcagc tacaccacag acggtgctgc caaggacggc aaagtcacct
     8221 ataccaatga ggacggctgg caggtcgtac tggcggacac tcatgatgcg gtgatctcct
     8281 cggcacggtt tgtcacggaa aatgacaagc tggcactgta cgtggacgac gacaccgccg
     8341 tgatcggact gtacgacaag gcaaaagaca agatgtggtg gtctactcct gaaaatgtgg
     8401 gacacgacaa gactgccacc aataccattg tagaggactt gtcctcctcg ctgaaaatga
     8461 tctacggcga acccgatgcc cgcagcacta ccaatatgcg ttccaagggc gatgcgaaga
     8521 tcaaggtcaa ggacaagagc agcggtgtga agatcaccta ttcgttcaag aaagcaggca
     8581 ttaccgtgcc ggtgacttac acgctggaag atgactatct ggaggcgaag atcgacaccg
     8641 ccgacatcga ggaggacgac acctccgaaa ccggcaagct gaccacttcc ctctccatgc
     8701 tcagcagctt cggggcggct tcttctactg atgagggcta cttcgtcatt cccgacggca
     8761 gcggtgctct gatccgcttc aacaacggca aaaagaccgc gaaatcttat accggctatg
     8821 tgtacggctc cgatgtgacg gcggttcccc tgacagaacc ggcagtgaca gagcaggttt
     8881 ccctgcccat gtacggcatc gtcaacggcg ataacgctat gatggtagtc tgcaccgagg
     8941 gcgactccaa cgccaagctg actgcaagcg tcagcggaca gtccaagagc agcttcaacg
     9001 tctgcggctt cgatttcacg gtgcgggatt cggataccta ttatatgtcc ggcgacaacg
     9061 gcacggcact gaccgtgttc gaggacggcg acatgaaaac cgacacgctg gcagtgcggt
     9121 actaccccct ggaaaccgag gacacaccgg actataccga tgtggcggcg gcataccgga
     9181 actacctcac cgaagaagcc ggcgtgacca ataccgtgga gaacacagat ccgtctttgt
     9241 acctcaactt ttacggcggc acgaaaaagg aaaaatccgt gctgggaatt cccgtaagca
     9301 tgaaaacggc tctcaccagc ttccagcagg cagaggaaat tctgcaaaac ctgtcggacg
     9361 gcggtgccaa gaacatgaag gtgcagtact acaactggac caacgccggc atttccggca
     9421 aggtggacat caaggcaaag gctgccggct gtctgggcgg aaacggcgac tggaacgacc
     9481 tgcaaagcta tgccgcgtcc aacggcgtga ccatttatcc ggtatcggaa aatgaaacgt
     9541 tccgttccgg cagcgggttc tatacatttc aggataccgc cgtccgcatc tccggctcct
     9601 atgcccgcat ctatgattac aatctggcat acggtactca gagtactgtg aacaagcctc
     9661 tgtccctgct ctcgccgtct gccttttcgg aggttgccga gaagcttacc ggcagcctgc
     9721 aaaagaaaga cctgaacacc ctttcgctgg gcagcctgac cactgctctt tacggggact
     9781 acggcaagca ggcgatctca cgagatgctg cccagcagct gctggaggac gcatatcagc
     9841 agatcactga tgcagacatt tccctgctgg cgaacggagc caatgcctac gctctgccct
     9901 atgtgcagga gatcacagac gtgccgctgc aatccagcgg attcgatgtg ttcgacgagg
     9961 acattccgtt ttaccagatg gtaatgcacg gggtgaaatc ctacggcacc agtgcggtga
    10021 atgcttcggc aacaccggaa gaaaccgtgc ttctcgccat tgcctccggc agcagcctgc
    10081 actttgacat gatcggcgaa gaaaccagta cgctgaaaga tacggtactg gacgggctct
    10141 attatgccag tgcggaaagc tggacagact acgccgcaca gagctatgca ttctccaagg
    10201 cggtgctctc cggactgggt gatcagacca tcaccggcta tgagagaaag ggcgatgtca
    10261 tcaccaccac ctatgaaaac ggcacggtgg tagaaaccga tctggcgaaa cagatcgtca
    10321 ctgtagacgg cacggcatac gccatggcgg actatgtgga ggaagggagc tggaacgaag
    10381 catgaaaatg gcatatgaaa aacgaaaaag catgtacggc tactgcttta ttgctctgtg
    10441 gttcgtgggt gcactgattt tcttcctgat ccctgtggta gagtcactgc tctattcctt
    10501 tcaggagatc acgccggaca ccggcggcat ggtcggcagc tgggtcggca tggacaacta
    10561 taactacgcg ttcaacgtgg acccgaacta ccgccagtat ctggtgaagg tgctgaagga
    10621 cactgcgtgg aaaaccccgc tgattctggt gttctccctg ttcgtggcag tcattctgaa
    10681 tcagaagttc aagggacgga cattctcccg tgccgtgttc ttcctgccgg tcatcattgc
    10741 caccggtccg gtgtataaca tcatcaacgg caacctgcaa agcaccggca acagcgatgc
    10801 cagtcagttt tccaccatgt tttccacaga tctgctgggc gaactcatgg aattcctcgg
    10861 catctacggc atttccgaca gcatgcagac caccattgaa atggtatcgg acaacatttt
    10921 cggcatcgtg tggaactccg gtatccagat cctgatcttc cttgcggcac tgcaaaacat
    10981 tccggtttcc gccaaggagg cagcccagat ggagggtgcc accgcgtggg aatacttctg
    11041 gaagatcacg ctcccctatg tcagccccat gatcctcgcc tgctttatct tcaccattat
    11101 cgactcgttc accgatccga acaacgtggt catgggacga atcctcgatt tgcagagcga
    11161 ctggcaatac ggaccggctt cggcaatggc gtgggtatac ttcgctatcg tgctggcggc
    11221 ggtggggatc attaccgcga ttctgaacaa gttcatctat tatgaagtag actaaaagga
    11281 gatgtcagtt atggaaacaa caaaaaacaa gccgctggca tccccgaaag aggatacgct
    11341 ggtgggcaac ggtatgagca agcgggaggc ccggaagatc cgcatgcaga gcatgaacaa
    11401 atccgagatc gcctatatgc ggcggaaacg ggtgttcgat gcggtgtggc cgtttttccg
    11461 gttcttcatt ctgttcggac tttgcttcgt cattctctat ccgctgatct acatgatcag
    11521 ctgtgcattc cgcgattctg cggacatgag tgacccgaca gtcatgtgga tcccccgtca
    11581 cctgacgctc aatgtcatca aggaaaccgc agatgtcatg gacatctgga acaccctgaa
    11641 aaccacgctt ctgctgaacg tcggctgctc gctggtgcag gtggcttcct gtgcactggc
    11701 aggctatggc tttgcccgct ttaagttcaa gggcaagggc attctgttcg gcatcgtcgt
    11761 catgatgatt ctggtgccgc cgcagatcat ctccattccc cagtatatgc tgttccggca
    11821 ttttggtatc ggcagcctga cagtgaacct gattaacacc aagctgtgta tgtacctgcc
    11881 ggcggcaatg ggcaacggca ttcgtgccgg tctgatgatc ttcatcttcc gccagttctt
    11941 caagggactg cccaaggagc tggaagatgc ggcatatctg gacggctgcg gaccgcttcg
    12001 cacttttgtg caggtcatga tccccaatgc ttcgtcctcg ttcctgaccg tattcctgtt
    12061 ctccgtggtc tggtactgga atgactatta tgtcagctcc acattcttca ccaagaacca
    12121 gaccattgcc ctgatgctga aaaatctgag caccctgctt tcccagcagc tgttcaataa
    12181 cgcagatgtc agccccagag agcagatcgt ctggatggaa gctggctgcc tgatcgcgat
    12241 cttcatcccc cttattatgt atgtgttcct gcagaagtat ttcactgagg gtatcgaacg
    12301 ctccggtctg gtcggataac gacacaacaa ttgcacatgt ttcaaagcac tctcctgttg
    12361 gagggtgctt tttgtgctgg aaaagggcag ggattctatg attcgtaatt gtagggacgc
    12421 ggcgtttatc agtttgtaca taagccttcc ttgaaagggg tggaggggtt cacccgtgaa
    12481 gtgtactttc tagtgcagtc atgtcgaaac cgtccggcgg aattcgctcg ttgccctcgc
    12541 tgtccgcctg tggcttgcca acgccggttg ttgcccttgc ctgtatttgt gacgttgata
    12601 ttcgggtgtt ttctggtgca ggcgggtaac tttattttgt atggcgtttg caagtctgct
    12661 gttataagcc tcccctgaaa ggggtggagg ggttcactcg tgaagtgtac tttctagtgc
    12721 agccacaccg aaaccgtccg gcggaattcg ctcgttgccc tcgctgtccg cctgtggctt
    12781 gccaacgccg gttgttgccc ttgcctgtat ttgtgacgtt gatattcggg tgttttctgg
    12841 tgcaggcggg taactttatt ttgtatggcg tttgcaagtc tgctgttata agcctccc
//
LOCUS       DAWBGR010000061        12696 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1511_length_12696_cov_4.964164, whole genome shotgun sequence.
ACCESSION   DAWBGR010000061 DAWBGR010000000
VERSION     DAWBGR010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12696)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12696)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12696
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1511_length_12696_cov_4.964164"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3082 a         3463 c         3248 g         2903 t
ORIGIN      
        1 gaaaaatttg acgacgcatc tgtcgcacaa tctttgtgcg gtattgccat aaaaacacgc
       61 tccgaaaccc gcctatggca ggttctgata ttcgtgcggg cagtcccttt tccggactgc
      121 cttgagaaag gacgatgtta tgacaaccag ttatcaacgt atcccgcttt cagatgccat
      181 tgcattttct gaaattctgg atcccaaact caaaaccaac tcgctgcgga tccaattcct
      241 ccagcctttg tcggagaaaa acgcagctgc ctgcggactg ggcggttctc tgatcgcctc
      301 cagcagcaaa gcctatccct ccaacgcgat catgaaccgc aagctgcaca tgctgtacgg
      361 ggcagatctc agttgcagca tttccaaaat gggtgatgta caggtcatca ctctgcggac
      421 ttcttccatt gctgaccgct atgctctgga acacgaagct gtcttttcgg aactcaccca
      481 gatcctgctg ggctgcattt ttgagccgaa tgtgacagac ggtgcatttg acgaaaccga
      541 gttccgcatt cagcagacca acctgctgga ctgcattgat gcggaagtca acgagaagcg
      601 gcagtacgcc atttctcaaa cccacaagac cgcattcgcc ggcgaaccgg ctgcgttttc
      661 ctgctacggc acccgcgaat ctgccgaagc catgactccg aaggctgctt acgaagcatt
      721 tcagcagctg ctccggacgg cacagatcga gatctacttt gtgggaccgg aagctgttcc
      781 ccagctggcg gacaccctgc ggcaggcttt cgctgccatt gccgaccgga agccggttcc
      841 cgtttcgttc ctttccccca gcccctgcaa gccggagcct gtcaccgtgc gggaagccct
      901 gccggtcaac cagtgcaaaa tggtcatggg ctggaaaacc agctataccg accggtatgc
      961 cctgaaaatg atgacgatgc tcctcggcgg tacacccagt tccaagctgt tcgccaacgt
     1021 gcgggaaaag atgagccttt gctactactg tgcggcaaac ttcaacgagt gcaagtcgtt
     1081 catgcaggtg gacagcggca ttgaaaccga gaatctggaa actgccgaaa ctgccattgc
     1141 ggcacagctg gaagccattt gcagcggcga catttcggac gaggaaatgc acagcaccat
     1201 gatgaccatt cacaacaccc tctgcggtgt gggcgatact gccagcagct atattgcatg
     1261 gtatttcggg cagttctgcc gcggcaccaa gctgacaccg gcggaggaag aagaacgtta
     1321 ccgtcaggtc accagggagg acatcatcgc ggcggcaaag tccatgcagc cggataccgt
     1381 ttatattatg gaatgcaaag aacaggaggc ggaataacat ggcagaaacc acgaaaaaga
     1441 cgatcgtttc cccccagacc ggtgacaagt acacccatat ccaccacaaa agcggactgg
     1501 agatcttagt ctgcgaaatg gagggctaca gcaccaccga agccatgttc ggcacaaagt
     1561 acggctcgat caacacccag ttcaagaccg cggcggacaa ggactactgc accgtgccgg
     1621 agggcatcgc ccacttcctg gaacacaagc tgttcgagaa cgaggactgc gatgtgttcg
     1681 acctgtacgc caagactggt gccaacgcca acgcgttcac ctcgttcgac aagacctgct
     1741 atttctttag ctgctccgac aacttcaagg cttcgctgga gatcctgctc tcctttgtcc
     1801 agtcgccgta tttcacgccg gaaagcgttg ccaaggaaca gggcattatc gggcaggaga
     1861 tccgcatgtg cgacgacgac cccggctggc gggtattctt caacatgctg tgcggtctgt
     1921 accagaagca tccggttcgc atcgacatcg ccggcaccat tgagagcatt gcggaaatta
     1981 cagacgatct gctgtaccgc tgctaccgca cgttctacaa cctccacaac atggtactgg
     2041 cagttgccgg aaagtgcacg gtggacgagg tgctggaggt tgccgaccgg ctgctgaagc
     2101 cctgcgaaga ccagaagctg gaaaccgtct ttccggagga aacactggac attgtacggg
     2161 aggaaacggt ggaatctgcc gcagtgggac agccgctgtt caatctgggc atcaagtgcc
     2221 agccgcggca gggactggac aatctccgtg cggaaatgct ggcggacatc accatgcaga
     2281 ccttatccgg cagttcctct cccctgtatc aggaaatgct gcaaagcgga ctggtcaacg
     2341 atagtttctc cagcgaggtc tttaacggcg acgggttctt taccatgatc ttcggcggcg
     2401 aatcccgcga cccgcaggcg gtgcgggaaa aggtcaccgc ggcaattctg gacgcacaga
     2461 aaaacgggct ggacgaggtc atgttcaacg agtccaaaaa gtccgcctac ggagaactgg
     2521 tgcggaagct gggcaatgtt tccgccgtca ccaacacgct ggtagcagct cacatggctg
     2581 gctgcggact gtacgacccc atgcaggtgc tgtctgaaat gacactggac gactgcaacc
     2641 gcttcctgcg ggaagaagtc gatgtccgcc gcatgagcct gtctgtcatc acgccggcag
     2701 aataatccaa aggagccgaa aacatatggc aactgaaaca atcgggacac tggactacaa
     2761 cgagatcgtt ttcccaaagc tgggcatcga tctccacgtc aacagcaccg ccttttcgat
     2821 cggcgggctg gacattcaat ggtacggcat tctcatcacg ctgggactgg tgctggcaat
     2881 gatctacgcg ttcacccaag tgaaaaagta cgggctgaat ccggaccgcg tgctggactg
     2941 catcatcggc ggcatcatcg gcggcgtggt aggtgcccgt gcctactacg tgctgctgaa
     3001 ctggagcgat tacgccggcg actggaaaag cattttcaac attcgcggcg gcgggcttgc
     3061 catttacggc ggcatcatcg gcagtctgct ggtgggcttt ctggtggcaa agatccgcaa
     3121 ggtgaagttt ctccccctgc tggacatcgt gggcatcggg ttcctgctgg gacagggcat
     3181 cggccgctgg ggcaacttct tcaatcagga ggcgttcggc tgcaacaccg acagcctgtt
     3241 cggcatgtcc ggcggacgga ttcaggagtg gatcaccgac cagtacccct ctaccaccta
     3301 ctttgccaac ttcggcacca cgctggacgc ttctcagccg gtacacccct gcttcctgta
     3361 cgaatccctg tggtgcctgc tgggctttgt gctgctggcg atttttgcca agaaaattcg
     3421 ccggtatgac ggacagatct ttctgatcta tatctgctgg tacggtgcag agcgtgccgt
     3481 ggtggagagc ctgcggacgg acagtctggt aatcggcaac gtccgcgtgt cccagatact
     3541 ggcgatcacc tgcgtggtga tttccataat tctgcaaatc gcaattggca ccaaagtgaa
     3601 acgcatgggc gtggactacc gcatgtacaa ggacaccaac gaatccaagc agatgctggc
     3661 agaatatgag gctgccaaat ttgtgaaaga gccggaggac gacaccaacg aggacaccgc
     3721 ttcgacagac gaagtggcag aaacagatac gacagattct tcagaatcgg acgaaacacc
     3781 agcagaaact gataccaacc aaacagaaaa ggagtaaata accatggcaa aactgatttc
     3841 gggaaaggaa gtttccaccc agattcgcca gaaaattcgg gaacaggcac agcagctgcg
     3901 ggaggaccgc ggtattcagc cggggcttgc cgtgattctg gtgggcaatg atccggcatc
     3961 gcagatctat gttcgcaaca agcagagagc ctgcgaggaa gtgggctttc acgcattcga
     4021 gtataagctg aacgaaaacg caacggaaga acagctgctg gatctgatcg gcgtgctgaa
     4081 caaggacaac aaggtacacg gcattctggt acagatgccg ctgccggatc acctcgatga
     4141 acagctggtc atcaagacca tttccccgct gaaagatgtg gacgcattcc accccataaa
     4201 cgtgggcaag atcatgatcg gcgactacga cttcctcccc tgcacaccgg cgggtgtcat
     4261 ggaactcatt gacagcaccg gcgtggagat cgacggcaag aactgcgtgg ttgtcggcag
     4321 aagcaacatc gtgggcaagc ccatggcaat gctgctgctc caccggaacg caactgttac
     4381 catttgtcac tcccacacca agaacctcaa ggaggtttgt gcatcggcag acattctggt
     4441 cagtgccgtg ggaaaggcac acttcatcac cgcggacatg gtgaaagagg gtgcgatcgt
     4501 gatcgacgtg ggcatgaacc acgacgaaaa cggcaagctt tgcggtgacg tggaattcgc
     4561 cgaagtggaa cccaaggcaa gctatatcac accggttccc ggcggcgtgg gaccgatgac
     4621 cattaccatg ctgatgaaaa acaccctgcg tgcagcagaa atgaaacaga agtaatttca
     4681 atcctctata tcaaaaacgc ccctccggtg cacttgccgg agaggcgttt ttttgatatg
     4741 ctgcatcttg ccgccgcagg gtgacatgcc ataacacaaa agagccactg cccgaaggca
     4801 atggctcttt ctatgaacaa caatttgaaa tgcgattatt cctttacgcc gcccagtgct
     4861 accccagcaa cgatgaatct ggacagaatc aggtagatca caatgatcgg gataacagac
     4921 agtgcaactg cagcgtactg tgcacccaga ttagggtgct tatcctgacc cagaacgctc
     4981 tggatcatga tcggcattgt gaaacgcttt tcattggaaa tgatgatcat attctgtgta
     5041 tagaagttgt tccagttctg tacgaacgcg aagatcgcct gtactgcaat tgccggtttg
     5101 cacatcggaa ttgcaatggt caggaaggtg cggaactcgc cgcttccgtc gatacgggca
     5161 gcttctacga tgtccagcgg gaaggaagac ttcatatact gacgcatata gaatacaacg
     5221 gcaggtgctg cgattgcagg aatgatcagc ggaatgtagc tgtttgccag acccagagta
     5281 cctaccatga actttacgaa accggtagat gtaacctgga tcgggatcat cataactgcc
     5341 agaataaacg tatatgcacc gtcacgaatc ttgaaatcgt aaacggtcag accataagag
     5401 gtcagtgcgg agaagaatac ggtcagaact gtggtacagc ctgcaatgat cagggagttc
     5461 cggtagccca gccaaacgtt aaagttcggg gactgcggga attcaccgcc ggcaatattc
     5521 ttgatattgg tcatcaaatt accgcccggc aggaactgca gcggctgaga acgcagtgca
     5581 gcgtccccgt gggttgcgtt catgaccatg atgtacagcg gaaccaagca gatcagtgcc
     5641 agcagaatgc agacaatgaa gcagccagcc gatttcagcc tttgcaccag aatgtaattg
     5701 tccttaacac caacgttttc cattatgcac ccagacctcc attcttagcc agcttctgct
     5761 gtttcttgta ttccttgacc agagccttac gctcctgctt cttccgatgc tcgtcttcat
     5821 cacggttcat acggaatacg agaataccca gagccgcagt gatgataaac agaattacgg
     5881 aagcggcacc ggcaatgccg tagtcaggct ggtgaacctt tgcaccggca cggaactgtt
     5941 cataaacgaa taccgcaacg gtacgcaggt ttacgttgat tttacctgca cctgtgtgat
     6001 acagatacgg aatatcgaac atctgcaaac caccgatcat ggaagtgatc agggtataaa
     6061 caaccatcgg tcgcagcagc ggcagggtta ccttacggag cacctgacca ctggaagcac
     6121 catcgatcgc agcagcttcg aacagagagg ggttgatacc catgatacca gacatcatca
     6181 tgatcatggt attaccaaac catagccatg tctgaataaa cataacgatg ccgcggctcc
     6241 aaacagcaga tgcttcgaag cttaccggat cgctgcctgc attcagcagc agtgtgttga
     6301 ttgcggacag ctggctgttg gaaataaaac gcatgaacag agctgcaaca gatgcagcgg
     6361 tgatgatatt cggcaggtac ataacaacct taaagaagcc cacgcccgga atgtgcagtt
     6421 tcgaatctgt gaaccagact gccagcagca gagacagcaa gatctgcgga atgaagttac
     6481 caaaccacag aatgatcgtg tttttgaacg aagtcaaaaa cagctcctgc attgcctttg
     6541 cacgacgacc cattccttca ccggaaccga acagaatggt agcatagttg tcgaagccga
     6601 caaatgtgcc cgcatctgca ccgttaccct gaaagctcag gataaaggtc tggatcagcg
     6661 gccagatctg gaagaaacaa tacacaagaa agaacggggc aatgaacata tagccatatt
     6721 tcgcatagct aatcgactta atacgacgtt gtgcagcgga tgccatttgc acacaccctt
     6781 tcaaaaattc agtagaatag taagagaaaa gataaacata cgcctatagg acgcaattat
     6841 cctataggct gtacgttcaa tcattcaaaa agcgttgaga agccgaagct tattcaacag
     6901 tgacgtcagt tacatcagct gcaattgcat ccaggaatgc gttcaggcag tcatcctcag
     6961 aagaggttac gccattgcag taatcctgaa ctgcgttctg caggtcagtg ttgatctttg
     7021 catcatactc agaagcaatg ctcatgtcga tcttgtcagc agagttgaac aggatctgga
     7081 actgatcctg accaccgaac agcgggttgc tgtttgtgcc agcttcagtt gtagcagaca
     7141 ttacgccctt gttgtttacg aagtcaccac gaacgtctac atacttctgc attgcatctg
     7201 catcagcagt catagtgtag atgaactggc cagcttcctt agcgttatcg gtcttcggat
     7261 atacgcagat ccatgtgccg ccccagaacc aggatgtcgg gccttcgcac agtgcatact
     7321 tgccgtatgt agagccgcct tcgccgcctt cagcctgttc cagagaaccg cccttagcga
     7381 ttgcccaagt cgggaagaag aaaccgaagg tcttgttagc ctgagcaccg tcagacatac
     7441 cctgcgggag ccagtcatca ttccactgag ttgcaccaga gatgtaaccc ttgtcgaagt
     7501 tctccttagc catagagatg aaatcaccta cggaagaagg atccagcttg ccgtcgttta
     7561 cccaagactt ggttctgtta ccgttggagt aagctctcca tacgccgccc agagagtcag
     7621 ccatagcagt ggtgttgcca gacttctcat atacggtagc tgcggtcttc cagaagtcat
     7681 cccagctgcc gacctgtgcc tgcatatctt ccggagaagt tacgcccaga tattcttcag
     7741 ccagatctgc acggtatgca tagccgcctg ctgcagcctg ccaagaagca cccttcagga
     7801 cgccgttctt gtccttgccc atagctacag tgtagtcata agcatttgca tacatgtctt
     7861 cagagatacc tactgcagac agcggagcag aatactcatc gttgttctga taagccatgt
     7921 tccagtcagc gtcgcagaag tacaggtcaa cgtcgtcgcc agacaggaat acctgtgcgt
     7981 actgttcgtt agcctcagta ccgttagaac caacgttctt gtagttgatc atgctcttgt
     8041 ccatgccggt tgcatcagca acgatgtcgt acatgtactg caggtcatct tcgttccagc
     8101 acaggattgt cagcttcttg ccgccgtcct tcagagtgcc gtccggttcg cccagagccg
     8161 gaacatcaac gttggtgtag ccagctgcgg actcaacaga agattcttct tcagcggtag
     8221 aatcttcagc ggaatctgca tctgcttcgc tgctgtcttc agcagaagac tcagccttag
     8281 actcagaagt gctggagctg tcattgccgc cgcatcctac gaatgcagtt gcagtcatag
     8341 ccagagcagc catcagagcc agtgttcttt tcaacttgtt cattggaaat ttcctcctta
     8401 aatatatata tcacacgttg tgccggtttg gtacagcagt gcaataaaca ataaaaaacg
     8461 ataccatgcc gtccgccgga gtttctccgc cgtgacatgc cctgttccca taaaaatgca
     8521 caaaacacgc ctagggaaca tttcttgtga taagcctaat atacaacaat tcgaaacaaa
     8581 tgtcaatgcc ttataccgtt ctttcatttt tttcagtgat acggtacaaa aatcagttga
     8641 tttttttgtg caatccgcca agtatttcga acatttctgt aaaaggttac aaactggtta
     8701 aaatgtcgag tttcctcaaa aaagtgcggc tactgggcaa gttcctcggt aatttgcaca
     8761 tagtcccgaa gctgcacgaa atacgcctcc gtcatgccgt ccacgtagag cagttcatat
     8821 accgacgaaa acgcctgaat ctgctgccgg aacgccacga tcttctccgc cagttccggc
     8881 tgcatcccgg gaatctgttc cagttccgcc gccgtcgcct gattcagatc cagcgggaac
     8941 gtcactgccg gtgctgctgc cgccggtggt tctgttgcag gcggagccgt ttctgccggc
     9001 tgcggtgctg ccgctggtgc gggagctcct gccggttccg gctctggctc cggttcggga
     9061 aacgtttcgt tttccagata gagcagatca taaatttcgt acagggtcgc ctcgccgatg
     9121 ccctccacct ccagaagctg ttcccgattg gagaacccgc cgatctggtc gcggtatgcc
     9181 gtgatgcgtt ccgccagcac cgccccgatg tggggcagct gttccagttc cgccgccgaa
     9241 gccgcgttca gttccagcgg aaaggtcgcg gtttcggctg ccggcgtggt gctttccgga
     9301 gccgtccggg tttcttggga agcctgtccg gcacgcttgg cagaagccgt tttcgtccgg
     9361 cgggtgccgg tgtgcagagt ggtttccgcg gcagaaacag ctgcttccgt gcccgtagtt
     9421 tccaccgttg cggcagccag ccgctgaggt cgggcggaaa gttccctttc ggaaacgtgc
     9481 cgtgtcccct gccagaacag cagccccaca gcacctgcca gcagaaccgt agttccgccc
     9541 cgcatcagcc atttcaagca ttcctgtttc atgcgttcct accccgtcct gcggaaaaat
     9601 ttcttccccg tcctgcggaa aaattccttg ctttgttgta atcctgcaaa aggttctccg
     9661 caagtccact gtctatttta gcacatttca cacaaatccg caagtgcttt tccggttttt
     9721 ttctatcttt aatcattttt caaaacattc gccgccgaat cgggatttca cttaaaaata
     9781 ttacgctttc cccttgaatt tcattcgatt tcaggcaaaa aaagcagggc accgaaaccg
     9841 gcaccctacc tcatttcaaa aatgtttcga gcctgtttcg ggaagccggc tgtcacgaga
     9901 atacgttaca ctcttgcaac gccggaatcc ttggctgcct gtgccactgc cttggcaacg
     9961 gctttgctga caccttcttc cagcgggctg ggaatgatat attccgcaga cagcttatcg
    10021 ggtgttacaa agtcggcgat tgccttggct gcggcgatct tcatggcatc gttgatgtcg
    10081 ctggcacgaa cgtccagtgc accgcggaag attcccggga atgccagaac gttgttgatc
    10141 tggttcggga agtcgctgcg tccggtgccc attactgccg caccggctgc cagtgcttcg
    10201 tccggcatga tctccggcgt ggggtttgcc attgcgaaga tgatcggatc ctttgccata
    10261 gacttcacca tttccggtgt cacgcagtgc ggagccgata cgccaatgaa aacgtctgcc
    10321 cctgccagca tttctgccag tgtgcctgcc ttgtggccgc tgttggtgat ctttgccatt
    10381 tcttccttct gagggttgag gttatcccgt ccctcgtaga tcgcaccgtg gcggtcgcac
    10441 agaatgacat ggtgcggatc cagacccatc gcgatcagca ggcggataat ggcaagtccg
    10501 gctgcaccgg caccgctggt caccacgttg atgtcgtgaa tgtccttgcc ggtgagcttc
    10561 agtgcgttga gcatagctgc cagtgtgaca acggcagtgc cgtgctggtc atcgtggaaa
    10621 atcggaatgt cacagcattc cttcagacga cgctcgatct cgaagcagcg gggtgcggaa
    10681 atgtcctcca gattgacacc gccaaaactg ccggcgatca ggcgaaccgt ttctacgatc
    10741 tcatccacat tcttggagcg aacgcaaagc ggaatcgcat ctacattacc gaaacgcttg
    10801 aacagtgcac atttgccctc catgaccggc atgccggctt ccggtccgat atcgcccagt
    10861 ccgagaacgg cggtgccgtc ggtgatgact gcgaccagat tgccgcggcg tgtcaggtca
    10921 tagcttttgg aaacatcttt ctgaatttcc agacaaggct ctgcaacgcc gggggtgtac
    10981 gccagagaaa gatcatcggc ggtttccagc ggtgcacgaa gtgtgacttc gatcttaccc
    11041 tgccatttgt agtgcagttt cagagattct gctgcataat ccataatttt ttcgtccctt
    11101 tccaaccaat caattgcctg tgttcggcga tttgccgaag tttttgtcag cggacagtgc
    11161 cggtgcatca gccccgccgc tgtacggcag ccgtctgcac agaggtctgt gccggcgtgc
    11221 gggtgctttt atactattta aattagagag ccatctggtc gattacagaa cgcagtgctt
    11281 cgccgctctt ctggaagcgt gcgatctcct cgtcagtcat cggcggtgtg atctcgtgtt
    11341 ctacaccatt tgcacccagt acgcacggca ggctcaggca aacgtccttt acgccgtagt
    11401 gtgtgccgtc ctgcagtgtg gaaactgtca gaactgcctt ggtatcccgc agaattgctt
    11461 cggtgatctg gctgacagac agtgcaattg cataatatgt tgcacccttg cacttgatga
    11521 cctctgcacc gccgtcgcgg acttcctgtt cgatctcgtc cttgtcgccc agattttcta
    11581 catttgcaca gtattcatca aacttcatgc ccgcaatgct ggacagggac cacgggatca
    11641 tggaagaatc gccgtgttca ccgaatacat atgcgtgaat gttcttgatg ttcagaccag
    11701 cgtgatctgc cagacaggta cgcagacgag aagaatccag aacggtaccg gaaccgatga
    11761 cctgcttggg gtccagaccg gatacgcgaa ttgcttcata ggtcagcacg tcaaccggat
    11821 tgctgactac gagataacat gcgtggggag catacttcac gatctccggc attacggaag
    11881 ccatgatctt tgcattggtc ttgcacagat cgatacgggt ctgacccggc tttctggcaa
    11941 taccaacggt gatgatgacc atatcggaat ctgctgcgtc cggataatca ccgtcgtgaa
    12001 tatcgcagga cgggcagaat gcattaccct gcataatgtc catagcctcg ccctttgcct
    12061 tgggcttgtt gatgtcgatc agcagcagtt cagagcaaag acctttgatc gccagagcaa
    12121 atgcgataga cgcaccaaca tttccggcac cgagaacggt aactttcttt ccttttttca
    12181 gcttcttttc cacttcagaa ttcctcctaa aaccttatct aggacaatat tgccctgaga
    12241 atccatactc catgcagatc gcagaaagga tctctcttta tcatacgcca cgcagtgaca
    12301 gaatgataga agaaaaccgc ctttgccctc tgcaacggca cagacagccg gtgcaggtac
    12361 aaaatccaac acccgtctgc ctatacaatt ttattataac atgaaccgta cttttttgca
    12421 agatgcaatc tggttttcca gaatttttgt cacttcatac agatttttcc cgttggaagc
    12481 cgacaatctc tacaaattac agcttgtttt ggaagtttga atcatagcct ttcctttatt
    12541 gaaatagcct cccctgaaag ggaaggggga ccagcgtagc tggtggaggg atttttatgt
    12601 ggaatctttt gcttaatgta aaaggtctac gatataatcc ctcagtcagc ctgcggctgc
    12661 cagctcccct ttcaggggag cctgaataaa aaaacc
//
LOCUS       DAWBGR010000062        12679 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1514_length_12679_cov_4.695421, whole genome shotgun sequence.
ACCESSION   DAWBGR010000062 DAWBGR010000000
VERSION     DAWBGR010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12679)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12679)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12679
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1514_length_12679_cov_4.695421"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    658..667
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3171 a         3297 c         3227 g         2974 t
ORIGIN      
        1 gaaaattttt tctgtttttt taattttagg ggttgacttt tatttgcagg tcttttcgtt
       61 tttccgatcc tctgcaacca tacgccgcaa ttccgcaaag gcagcgtcct ctccctgctc
      121 cgccagcaga gtcagcagcc gcatcagttc ccgttctgtg ttggcgtgca gcagtccccg
      181 cttgcgggaa gtggatttct gaaaatacgc cagcggacag gtctggtcat aggcatcttt
      241 catgtagatc ttgctggcgg caactctgtc acagaacatc tccgccagaa accgcaccgg
      301 catttccacc ggttccactt gcttggttct ggggttatag tccgaccagt actcgaaatg
      361 gtgacggttc cgccccttgt ggtgcatcca tgccagcgaa tagcccaacg cctctcgttc
      421 ggcttcgttg ggactgcgtt tgccgtcctg atagtaccgg acaccctgcc agaattctgt
      481 cgggctgtac ttggacaaat catgccgcag cccctgccag aaaatccccg cccgccggca
      541 gtgccgcatg acagtgtgcc gatgtcgggt aatggtacag aaatgccccc aaaaagaatg
      601 ccagcccatg ggaaaaacct cctgttgcag atgatacttt tagtatagct ttttttgnnn
      661 nnnnnnnttg ggggaaaagt caaggcagcc atggctcaaa gcttgcacgt aaattttagg
      721 aaacactgaa taaaacttgc aagcagattg cgtgcaaagt cgccagacgc tctttgtgcg
      781 gtattgcctt atagttcaat tccaaaaaaa tttctccccg cccttgactt ttccgaaaaa
      841 tctggtacat tccatagatg cggaagggaa atcacaagga gggatttcca tgaaagaaaa
      901 aatctgtatc ggaatcggtg ctgccggcgg gctgttggct gcggctctcg gcggctggga
      961 cttgggctta caagtgcttc tgggctttat ggcggcagac ctcggaacag ggctgcttac
     1021 tgccggcgtg ttccacaatt ccccgcacac caaagacggc aggctggaca gccgctgctg
     1081 ttttcagggc atgtgccgca aggcagaaat gctggtgttt gtcgggacgg caaacgcggc
     1141 ggacttactg ctgaacagtc acttcctgcg aagtgcagtc atcattggct tctgtgccag
     1201 cgaagccctc tcgcttacgg aaaacgccgg acgtatgggc attcccctgc cggcagcgtg
     1261 gaaaaatgct gtcagtctgc taagccgcaa ggcacagccg gacagcccgt cggaacagga
     1321 gtgatcgcta tgcagataca aaacgcctac ctcacccaca accgccccaa aacacgccga
     1381 agccgtacca ctgccattgc cgtgcactgg gttgcaaatc ccaacagcac cgccatggca
     1441 aaccggaact actttaacac caccagccgt gccgtttcca gcaactatat tatcgggctg
     1501 cagggggaaa ttctatgctg cattcccgat gaggaaacca gctggtgcac aaatgaagca
     1561 aattcctata cggtcagtgt ggagtgctgc catccggact ggacgggctg cttcacggga
     1621 aaaacctaca ccagtctggt ggaactgact gcccagctgt gccagaaata tcatctggat
     1681 ccgcagaacg gcggggtgat acgacactac gacgtgacac gaaagatctg tccaaaatgg
     1741 tttgtccctg ccagccgcgg cgggtcggat accaacgatg agcggcattg gaaacaattt
     1801 ttgcatgacg ttgcccggca gatgcaacgc ggcagtactg ccatttccac accggcagca
     1861 gagccggaca gctatcgggt tcgcgtgact gtcgatgccc tgcggatccg taagggagct
     1921 ggtaccagct atgcgatcac cggctgcatt cgggacaagg gtgtctatac tattataaag
     1981 acctgcggca actggggcaa gctgaaatcc ggtgccggct ggatctgtct gggatactgc
     2041 cggaagcttt gattgcagat cctgctgaac gacaaaaagc ctgtcgggga gcaattctcc
     2101 ctgacaggct tttgcaatat tctctacctt tttaccagag tgttccgttt actttttcag
     2161 atccataagc atggcgttca gtgcactcaa gaatgcagag atcgaagagt tgatgatgtc
     2221 cgaatgcacg ccaacgcccc aataggtctt gccgttcttc tccagtccca cataagccac
     2281 tgccttggaa ttggagcaaa cctccagtgc atgttctgtg taggtggtga gagagaagtc
     2341 catattcagg tgtgccttta ccgcattgga aaccgcgtcc agacgaccgt ttccgtcacc
     2401 gtgtactgtt tctgtgctgc ccttatagga aatggttgcc tcggtgccaa tggcattttc
     2461 gcccttctgc acgaagtgga cttccggaat ggaaaccggt gtttccacgt tgacatagtg
     2521 cttggtgaag atgtccagta cttcttccgg cagcaattcc ttgtgggcat ggtcggaaac
     2581 gcccttgacc agatagccga aagattcccg catcttctgc ggaatgtgat agccgtactt
     2641 ggtttccatg aggtagctga caccgccctt gccggactgg ctgttgatac ggatcacgtc
     2701 tgcctcgtag acacggccca catcttccgg attgatgggc agatacggca cgttccagtg
     2761 cttgcagtcg tgttcctctc tccagtgcat gcccttggca attgcgtcct gatgagaacc
     2821 gctgaacgcg gcaaatacca gctgaccgct gtacggcgaa cgctcataaa cgtgcattct
     2881 ggtgacacgc tcgtaaacct ctacgatctc cggcatgttg gagaaatcca gttccggatc
     2941 cacgccctgc gaatacatgt tcagagccag tgtgacaatg tccacgttac cggtacgctc
     3001 accgttgccg aacaaggtgc cctcgatacg gtcgccgcct gcaagcagac ccagttcgct
     3061 gtctgccacg gcacagccac ggtcgttgtg agggtgcagg gagatgatga cattttcccg
     3121 acggtacagg ttgtcgctca tgtattcgac ctgattagca taaacgtgcg gcatggagtg
     3181 ggaaaccgtg accggcaggt taatgatgac cggatcttcc ggtgtcggtt cccaaatttt
     3241 caatacggcg ttgcagatct ccagagcgaa ttccggttcg gtgccagtaa agctttccgg
     3301 agaatactca aaccggaagt gtccctcggt ctttgcccga tattccttgc agagatttgc
     3361 ccccagttcc gccagcttga tgatgtcttc cttgctcttt ttgaacacct gttcccgctg
     3421 tgccacggaa gtggaattat agaggtgcac aatggcatgt tttgcaccct tcagggcttc
     3481 aaatgtcttg gcaatgatgt gttcccgtgc ctgtgtcaat acctgaatgg tgacatcgtc
     3541 cggaatcata ttgttttcga tcagagtgcg gcagaattcg tattcggttt cagaagcagc
     3601 cggaaagccg atctcgatct ccttgaagcc gatctttacc agatagcgga agaattccag
     3661 cttttcatcc agactcatag gcgtgatcaa agcctgattg ccgtcacgca gatcgacgct
     3721 gcaccagatc ggtgcctttt cgatcattgt cttttgtgcc caacgcatag gcgggttctt
     3781 taccggaaaa aactgcggtg catactgttc tacgtttttc ataagataca ttcctttcct
     3841 gttgtgtgta ggtgatctac cctgtcatgt gatctgcggc gatctgctct gtcaacaaaa
     3901 aaagcccctt ccatacagat attttcatct gtacaaaaga gacgaagatc tatttttctc
     3961 cgtggtacca ctctttttga cgcagcgtgt gcgtccactc atcacatctg acaatgtgta
     4021 ctctgctaac ggagagtatc cggcacagcc tactgatacg ggataatgcc cgtctgttcc
     4081 gcctgcggct cggggaccag ttatcctgaa cgccctgctg tcagctcaca ccagccgccg
     4141 actctctgcg atgcagaatg cgttctcttt tcttcccgtc aaagcctttt tcatgataca
     4201 accagcaatc ccattgctgt gaatatcttc agtatagcag ttttcgcctt gtttgtcaag
     4261 catttttgcg aaaattcttt ggatttcacg aaagacgctg aatatttctg ttttacagtg
     4321 ctgtatattg aacaaattgt atccaaaatg taaaaacaaa agacagacac ttgcatttgc
     4381 gaaaacatag tgctatacta gacacatacg aacagcaatg gcggcgaata tcgccgtccg
     4441 gcggcggagt cgtaaagtct ttcggaaagg gagtatcctc acatgtacag tgacatgatg
     4501 tgccggctgg cagcacgagt gatgatcgtg gaactagacg ctcgagtcat tttgacccga
     4561 agcggaattt gtgctgtaca gaccgtggtt ccaaagacaa atgcgaacgg ctgtttttcc
     4621 gccgcaacgc ttgcatgaag attggaagct ttcggtatga cagccgaagg ctttttttat
     4681 aggacaccgc aaaatgtctt gctgtacagc cgcctgcgtg cctgtacttc tttcatagaa
     4741 agcgtgccgc atctgccggc atatcgcttc gggtctatac caatcgtatt tttgctaaca
     4801 gaatcagcaa ttcttctcaa atatcggagc ttttgcctaa atggaaaagt ttcccagtaa
     4861 aaaggtgaaa aacaaatgaa attgaaaggt gcacaaatca tcgtagaaac cctgatcgaa
     4921 cagggtgctt ctacggtatt cggctatccg ggcggacagg tcatcgacat ctacgatgcc
     4981 ctctatctgg cacaggaccg gatccatcac atcatcacag cccatgaaca gggtgccgca
     5041 catgcagcag acggctatgc acgtgccact ggcaaggtcg gcgttgtcat cgccacctcc
     5101 ggtccgggtg ccaccaatct ggtcaccggt atcgccactg catatctgga ctctgtgccg
     5161 ctggtagcga tcaccggcaa cgtgccgaac tctctgatcg gacgggacag ctttcaggaa
     5221 gtggacatca ccggcgtgac catgccgatc accaagcaca acttcctggt aaagcgtgtg
     5281 gaagatctgg cagataccat tcgggaggca ttccgcattg ccaagtccgg cagaccggga
     5341 ccggttctgg tagacgttcc caaggacgta cagaacgccc tgtatgagta cactgcacag
     5401 aagccggtgg aaaaagatcc ggttccggca gtcaacgaca gcgagatcga aaaagcaatc
     5461 gaaatgattc tggctgccga aaagccgtac atctacatcg gcggcggtgc aattaccgac
     5521 gatgccggtg cggaagttct ggctctggca gaaaagatcg acgcaccgat cggctgcacc
     5581 atgatgggca tttccgcagt gccgtcctcc aatccccgct ggctgggcat ggagggtatg
     5641 catggacact atgcctcctc cattgcacag aatgaagcag acctgatcat cacagcaggc
     5701 tgccgcttca gcgacagagg caccggcaac actgcaaagt atgccagaaa tgccaagatc
     5761 attcacatgg acatcgatgc ttctgagctc tgcaagaaca ttccggtaga actgggcatg
     5821 accggttcca tgaaggaaat cctgcaaaag ctggcagaaa aactgccgca gcagactcac
     5881 gccgactgga tggcacgtgt ggctgaatta caggaaaaga ccagagaact ggaagcacag
     5941 aatccggacg aactgacacc gttcactgcc atcgatgcac tggcagaagc ggcagacaag
     6001 gaaacgatcg ttgccacaga cgttggacag catcagatgt gggtggctca gcactatccg
     6061 tttgaaacac cccgcacctt tatttccagc ggcggtctgg gcaccatggg ctttggtctg
     6121 ggtgctgcga tcggtgccgc aactgcaacc ggcaaaaaga ccattctggt caccggcgac
     6181 ggcagcttcg gcatgaacct gaacgaactg gctaccgcag tcaccaacca gacaccggtc
     6241 gttgtattca tcatgaacaa cggcgtgctg ggcatggttc ggcagtggca gaccttgttc
     6301 ttcgacaagc actattccaa caccacgctg gaacgcaaga ccgacttcgt caagctggca
     6361 gaagcattcg gcatgccggg ctatcgtgcc gtgaatatgg cagaactgaa agaagtcctg
     6421 cccaaggcgt ttgccgaaaa aggaccggtt ctggtagatt gtgcgatcga ctgtgacgca
     6481 ttcgttcttc ccatgctgcc gccgggcggc tgcatcgatg atattatcgt cgatatttct
     6541 gcaatgtaaa ggggtgtgtc atcatggatc gaatcgaacg ctttgttatt tccgtcctcg
     6601 ttgcaaacca ttccggtgtg ctgtcccgcg tttcgggcat gttcaccaga cgaggcttta
     6661 atattgatac tctgacagtc ggagaaaccg aatcaccgga attctcccgc atcaccattt
     6721 ccacccactg tgatcccggt atctgtaacc agatcgtcaa gcagctggaa aagatgtatg
     6781 atgtcaagac cgtcaaggtc atggaacagg atcagacagt ggtacgcgag ctgctgctca
     6841 tcaagatcaa caatgtggca gacaagcgtc aggatattat gtctgtggtc gatgtcttcc
     6901 gtgcgaagat caccgactat tctccggagg cattggtcat cgaaattcgc ggcgagtcct
     6961 ctaagatcaa cgcttttatt gagttgctgg aaccctacgg catcatggaa atgtgccgca
     7021 ccggtctggt cgctcttgaa agaggcggct catgcctgaa agatgcgtga gcagtcgtat
     7081 ataaatggat cagcagactg cacggatgct ccggcggtac tgcgtcggaa atgaaaaatc
     7141 gtttccgaaa cccaaattta ttcaaaggag tctttcacaa tggatcatgt tgcaaaggta
     7201 ttttatcagg aagactgcga cctgtcgctg ctgtatggca agactatcgc tgttatcggc
     7261 tacggcagtc agggtcacgc acacgcactg aatgccaagg aatctctggg cgataaggcc
     7321 agagtaatca tcggtctgta caagggcagc aagtcctggg caaaggctga ggcacagggc
     7381 ttcgaagtat tcaccgcagc agaagctgca aagcaggctg acatcatcat gattctgatc
     7441 aatgatgaaa agcaggcagc aatgtacaag aaggacattg caccgaatct ggaaccgggc
     7501 aatatgctga tgttcgctca cggctttgcg atccacttcg gtcagatcat tccgccggca
     7561 gacgttgacg ttaccatgat cgctccgaag ggaccgggtc acaccgttcg ttcggaatat
     7621 caggctggca agggcgttcc gtgcctcgtt gctgtacagc aggacgcaac cggcaaggct
     7681 caggatctgg cactggctta tggtctggct atcggcggtg caagagccgg cgttctggaa
     7741 accacattcc gtaccgaaac agaaaccgac ctgttcggtg aacaggctgt tctgtgcggc
     7801 ggcgtttgtg ccctgatgca ggctggtttc gaaactctgt gcgaagctgg ttatgacccg
     7861 agaaacgctt acttcgaatg cattcacgag atgaagctga tcgtagacct gatctaccag
     7921 agcggtttcg caggcatgcg ttactccatc tccaacactg cggaatacgg cgactacatc
     7981 accggtccga agctgattac cgaggaaacc aagaagacca tgaagcagat cctgaagaac
     8041 attcaggacg gttccttcgc aaaggaattc ctgctggata tgagcgaagc aggcggtcag
     8101 gcacacttcc gtgctatgcg taagctggct gctgagcatc cgtctgagaa ggtcggcgaa
     8161 gagatccgta agctgtacag ctggaatggc gaagacaagc tgatcaacaa ctaatcagca
     8221 catttgctac agcaatcccc catttctgaa tcgatgggat acgcagggat aaaaccataa
     8281 cgcagaaact gctttcctga cgcaatatca ggaaagcagt ttttggatac acagcaaatc
     8341 cattgtgaca cacggaaaag tatttttccg aaactatatg aggtgaaaaa aatgaacggt
     8401 aatttttact gtgagggtgt cgataaggca ccccagcgtt ccctgttcca cgcactggga
     8461 atgaccaagg aagaaatgga acgtccgctg gtcggcatcg tttcttctta taatgagatc
     8521 gtaccgggac acatgaacat cgacaaaatc gtggaagctg tcaagctggg cgttgccatg
     8581 gctggcggta caccggtagt atttccggca attgcagtct gtgacggtat cgccatggga
     8641 cacaagggca tgaaatattc tctggtgaca cgtgacctga tcgccgactc caccgagtgc
     8701 atggctctgg cacatcactt tgatgcactg gtcatgatcc cgaactgtga caagaatgtc
     8761 cccggtctgc tgatggctgc cgcaagagtg aacgtgccga ctgtctttgt cagcggcggt
     8821 ccgatgcttg ccggtcacgt caagggcaag aaaaccagtc tgtccagcat gtttgaagct
     8881 gtcggctctt atgctgccgg caagttcact ctggaggacg tggaagaatt cgagaacaac
     8941 gcctgcccca cctgcggctc ctgctccggc atgtacaccg caaactctat gaactgcctg
     9001 accgaagtgc tgggcatggg gctcagaggc aacggcacca ttccggcagt atattctgaa
     9061 cgtatcaagc tggcaaagca ggctggtatg gcagttatgg atatgtaccg gaagaatatc
     9121 cgtccccgcg acatcatgac cgcagatgct tttgaaaatg cactgaccgc agatatggca
     9181 ctgggctgct ccaccaacag catgctgcac ctgccggcaa ttgccaacga atgcggcatc
     9241 aagatcgatc tggacatggt caaccagatc agtgcacgca ctccgaacat ctgccacctt
     9301 gctccggcag gtcacgcata catggaagac ctgaacgaag ccggcggcat ctatgccgtt
     9361 ctgaacgaac tgaccaagaa gaatctgctc cacaccgatg tgatgactgt caccggcaag
     9421 accatggcag agaacatcaa gggatgtatc aacaagaaca ccgacatcat tcgcccgatc
     9481 gatcagccgt acagcgaaac cggcggtatt gctgtcctca agggcaatct ggctccgaat
     9541 cgctgcgttg taaagcggag tgccgttgca aaggaaatgc tggtacacaa gggaccggca
     9601 aaggtattca acagcgaaga agaagctatt gcagcgatca ccggcggcaa gatcgtcaag
     9661 ggtgatgtcg ttgtgatccg gtacgaggga cctgcaggcg gtccgggcat gcgggaaatg
     9721 ctctccccga cttctgcaat cgccggcatg ggactggaca aggacgttgc cctcatcaca
     9781 gacggccgct tctccggtgc gacccgcggt gcagcgatcg gtcacgtttc tccggaagca
     9841 gtcaacggcg gtactattgc ctatgtacag gacggcgaca tcatctccat tgacattccg
     9901 aaccacagca ttcagctgga agtttccgac gaggaactgg cagaacggaa aaagaccacc
     9961 aagattctgg tcaaggaaaa cctcaccggc tatctgaagc ggtattccaa gatggtttcc
    10021 tctgcggatc agggtgcgat catcaaccgc atggactaaa tcaaacaaag gaggaacttc
    10081 ccctatgggt atgacaatga cacaaaagat ccttgctgca cacgccggct tggattctgt
    10141 cgaagccggt cagctgatcc tggtcaatct ggatcgggta ctgggcaacg acatcacttc
    10201 ccccgttgca attcgggaat tcaacaagct gaacaaggac aacgttttcg ataaggacaa
    10261 ggtgaccatg gtcatggatc actttgcccc gaacaaggac atcaaggctg ccacacagtg
    10321 taaaatgtgc cgcgatttct gcggcgaaaa ggagatcacc cacttctatg acgttggtca
    10381 aatgggtatc gaacatgccc tgctgccgga aaaaggactg gtcgtttccg gtgacgttgt
    10441 tatcggtgca gactctcaca cctgcacata cggtgcactg ggtgcatttt ctaccggtgt
    10501 cggctctacc gatatggcat gcggcatggc gatcggcaag gcttggttca aggtgccgtc
    10561 tgctatcaag tttgttctca agggcaagct gaacccgtac atctccggca aggacgtgat
    10621 cctgcacatc attggcaaga tcggcgtaga cggtgctctg taccgctcta tggaattcac
    10681 tggcgagggc gtggcttctc tgtctatgag cgaccgtctg accatgacca acatggcaat
    10741 cgaagccggt gccaagaacg gtatcttcga ggttgacgat cagacactgg cattcgtggc
    10801 agagcacagc gaccgcaagc cggttgtcta taaggctgac gaagatgccg tttacgatga
    10861 agttatcgaa atcgacctgt ctgagatcaa gtccaccgtg gcattcccgc acctgccggg
    10921 caacacccgc accattgacg aagtgggcga ggtgaagatc gatcaggttg tcattggctc
    10981 ctgcaccaac ggtttctaca aggacatcga agaagctgct tctattatcc gcggcaaaaa
    11041 ggtggcaaag aacgtccgtg ccattatcat tccggcaaca caggcaattt acctgaaggc
    11101 tatggaaaac ggtctgctga aggatttcat cgaggctggc tgcgtagtat ctaccccgac
    11161 ctgcggcccg tgtctgggcg gacacatggg cattctggct gccggtgaac gtgcagtttc
    11221 caccacaaac cggaacttcg taggcagaat gggtcacccg gaatctgaag tatatctggc
    11281 aagtccggcg gttgcagcag caagtgccat tgccggcaag atcgtttccc cgaaggaggt
    11341 actgtaatca tgcaactgaa cggaactgtc atcaaatacg gcgacaacgt ggacacggac
    11401 gttatcattc cggcacgtta tctgaacacc attgacaaga aggaactggc ttctcactgc
    11461 atggaagatc tggataccac cttcaccagc cgcgtgaagc cgggcgacat catggtcggc
    11521 ggcgaaaact tcggctgcgg ttcttcccgt gagcatgccc cgattgcaat caaggaaagc
    11581 ggcattgctc tggtcattgc caagagcttt gcccgcatct tctaccggaa ctctatcaac
    11641 atcggtctgg cgatcgtgga gtgtccggag gctgtagacg ctatcagcga gggcgacacg
    11701 gtggaagcag atctggacaa cggcatcatt cgcgatgtca ccaccggcaa ggaattccat
    11761 gtggcaccgt ttccggaatt tatccagacc attatcgcaa acggcggtct gatcgagtcg
    11821 atcaaaaagg gtgctgtgaa gtaagcggtt ctctgaaacc ctatgatata caaaaagctg
    11881 ttgtaccatt tgtttggtac aacagctttt ttgttgtctg aaaactggaa tttatgcttg
    11941 ctttttagaa tttctgatat atattgcgga tgttataaca accattagaa taagaatatg
    12001 caaaatagaa tgataaacat ctggtatata tcttccatca atttcaagcc actttatagc
    12061 ccctgaaaaa cactttatct taccttcaag tgaacccatt atccctttac caaaaatagt
    12121 ataccattcg tggcataaga attgaactac aaaccatatt gatgtccata ccgcaacagc
    12181 ccattttccg aatttttcct taaatataaa taatattata gttactaaat aaataagaaa
    12241 aaatattcca tcatctttat atgagcgtgc gactaaataa gtatttctga aataaatacc
    12301 tgtcatatca agaaaaaacc agaacagaag caatatttga ggtattatac aatatctttt
    12361 cttcattatt taccacctaa attccgattt accgcatttc cattatacca cacagcccca
    12421 acgcagtcaa tccgccacaa atttcaccgg cacagtgccc gctatgtgtt cttctcatgt
    12481 ggaaaaacta gaagttgtct atttctcctc ttcatcaata cctagttcat cgtgtatttg
    12541 ttcttcttca atatcttcgg tagtgacttt tcctgtaata tccctatagg cttcttttat
    12601 tccatttctg acagcccatt taactataaa atataatgca agtaagacga ttagtgctgt
    12661 gccaaaacta attcctaat
//
LOCUS       DAWBGR010000063        12346 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1544_length_12346_cov_5.276788, whole genome shotgun sequence.
ACCESSION   DAWBGR010000063 DAWBGR010000000
VERSION     DAWBGR010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12346)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12346)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12346
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1544_length_12346_cov_5.276788"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2806 a         3505 c         3049 g         2986 t
ORIGIN      
        1 aaaatttgca agcagatggc gtgcaaagtc gccagacgct ctttgtgcgg tattgcccta
       61 aactcaatcg atctcgactg cgatcttctg attgtttttc gcagcaccgg aacgcatgat
      121 tacacgaatt gcctttgcaa tgcgtccctg cttgccgatg acgcgtccgg tatcctccgg
      181 tgcaacgtgc agatgataaa cgatcacgcc ttcttcattg ggtgcgtcct ctgtgatctg
      241 aatggcagtc gggtcttcca ccaaaccggc aacgatcact ttcaacagtt cctgcatgtg
      301 tcatacctcc ttgtgaacaa cagttacggc aataccgcac aaagtcgcca gacgctcttt
      361 gtgcggtatt gccttacatg cgattgctcc actggtcagc cgtagcacac aggatacggt
      421 tcaccggcgg agcaatcctg ttctgccatt gcagaacaac cccgcactgg cactttccct
      481 aagtgtaaaa cttagagaac gccttccttc ttcaggatca ccttaacggt atcggtcggc
      541 tgtgcaccat tcttcagcca agtctttgcc ttgtcagcgt ccagattaac aactgtcggc
      601 tccttggtcg gatcatagta accgatctcc tcgatgaagc ggccatctct cggataacgc
      661 tcatcagcaa caacaatacg atagaacgga gccttctttg cacccattct tctcagtctg
      721 atctttactg ccatttgaaa ttcacctccg tgtttattct tgacaaatgt caaatctatc
      781 tccagcagtt tcctgcggga tctcttattt cttcggcggc atcagccccg gcggaacgcc
      841 cgggataccg ccctgcaagc tttgcagctg ctccggattc atgtttgcca gacgcttcat
      901 ggcacggcgg ttcatcttgc cgcctttgcc tgccttgccg cccagctgct tcatcatctt
      961 ctgcatctgg tcgaactgtt tcagcagacg gttcacatct tccaccttgg tgccgcttcc
     1021 ggcagcgata cgccgcttgc gggaggggtt gatgatcgag ggctttgccc gttccgcctt
     1081 ggtcatggac gtgatcattg cctcaatgcg gacaaactgc cggtcgtcga tgtcggcatc
     1141 tttcagcttg ctgcccacgc cggggagcat gccgataatg gatttcaggg agcccatttt
     1201 ctggatctgc cgcagctgtt ccagcaggtc gtccatgtcg aagcggtttt cctccagctt
     1261 cttcgccatt ttctcggcat ctttctggct gatggtgttt tccgcctttt cgatcagcgt
     1321 cagcatatcg cccatgccca gaatgcggga agccattctc tgggggtgga actgttcgaa
     1381 ttcgtccagc ttttcgccca tacccacata cttaatgggc ttaccggtga ctgccagcac
     1441 ggacagtgcc gcaccgccgc gggtgtcgga gtccagcttg gacatcagca cgctgtcaat
     1501 gcccagtgca tcatcaaatg cctttgccac gttgacggca tcctgacctg tcatggcatc
     1561 gaccaccagc atgatctcgt tggggttggt cagttctttc agatccttca gttcgtccat
     1621 gagctcggtg tcgatgtgca gacggccggc ggtatccagg atcaccacgt cattgccgtg
     1681 atccttggca aaccgcagtg cctctttggc aatgatccgc gggtcgatct gtcccatttc
     1741 aaagaccttg acaccggcac gctcgcccac cacctgcaac tggttgatcg ccgccggacg
     1801 gtaaatatcg catgccacca gcagcggata atgcccttcc tttttcagca tcttcgccag
     1861 cttggcggaa tgggtcgtct taccggagcc ctgcaagccg cacatcatga tgacgcaggg
     1921 cggcttggac gggaactgga tccgggcgtt ttcattgccc atgagattgc acagttcctc
     1981 gttgacgatc ttgatgacct gctgtgccgg tgtcagactg ctgagcactt cttccccgac
     2041 tgcacgttcg gaaacctttt tgacaaaatc cttgacgacc ttgtagctga catccgcttc
     2101 cagcagagcc atgcgaactt cccgcatggc ttccttgaca tcactttcag tcagcttgcc
     2161 gtgggatttc agctttttga acacctgatt caattttccg gaaagtccct caaatgccat
     2221 gtgatctcct cccctgcaaa ttttttcaga gcagctccag cccgtggtgc accgcttcca
     2281 ttgcctcgcg gcaggcggtc tgcacctgcg gctggtctgc cgtctgtgtc tggatcgact
     2341 gtaacgtcga ggcgatcgca ctgtagtttt cccgcagagc gttgaacctc gccgcaaact
     2401 gcagcttgtc ttccatttcc tgcaaaatct gttcgccacg cttgatgccg tcccgcaccc
     2461 cctgacgggt gatgccgacg atctccgcga tctcgctgag ggacaggtct gcacaataat
     2521 acagttccgt cagccgatac tgatgcggtg tcagaaattc gccgtagcaa tccagcagca
     2581 tggcaaatcg caaatccttg gacattccca gccgcctcct gcctgtaaaa gtcatctgct
     2641 ttacaagtat acccgatttt ccgccgcttg tcaagccctt tttcttcgtg aaactgcaca
     2701 aatttgaaat cccacttttt ccggttatgc aatatatttg cttatttttc atgttttatt
     2761 tgcattctcc ttgacattct ccaaaaatac gcctataata gatagaaaag cacagaacaa
     2821 caccgcacaa ggcttgtgcc gtgttgccac aggaatattc cggcagttct cgccggaaac
     2881 cgcgaaagga atgacaaaaa tgacttttga tgaaatgttt gcacaggcaa aggcactgct
     2941 ggaaaacgcc agtgccaagg acaccgacac cgccgtatcg atacagtttg atgtttccgg
     3001 tgacggctgc ggtgcattct atgcagtagt ttccgcagac agcgacaagc tgaccattga
     3061 gccgtttgac tataaggatc acgatgtact ggtatccgca gattccgctg ccctgctgga
     3121 cgctctgcgt accgcagaaa ccgctgcact ggaactcgcc ggtgaatggg aaaagatcgt
     3181 ggcattccgc agcgttctgg atactctgcc caagccgaag aaggaggctg ctcccaaggc
     3241 aaaggctccg gctgcgaaga aagcccctgc aaagccggct gcaaaaaagg cagaagcacc
     3301 gaagactgct gcgactgtaa aaaagacaga accggtaaag tctgctgcaa aaaaaccggc
     3361 gactgcaacc gcaaagaagg aaaccgtcaa ggctgaagct cccaaggctg ctgcggctgt
     3421 caagaccgaa ccggtaaagc cggcagtgaa aactgcttct gcaactgcaa aggttcccgc
     3481 tgcaaagccg gctgcaacca ctgccaagac tgctgctgcg aagaacagca aaaagtaaag
     3541 gcaataccgc acaaagactc ctcggggacg acacccatca caaaaaacag ccgtgacaac
     3601 accgtgcaaa gccgacagac cggctctgtt cagcgttgcc ctcagagaaa ggagtccttg
     3661 aaatgccgca gatcaccgta acagatgaac agggaaattc ttattttccc acctatccca
     3721 gacgggcaag aggacttgtc aaaaaagggc gggcaagatt catcagtgca aatacgatct
     3781 gtctgaactg tccgccgcag aatcaggagg aacagcagat gacagaaaat acaaatacaa
     3841 gcagcctcac agctcccact gccgctgaaa tttttgcaga attgcagcag atccggcagg
     3901 atacccagca tctttcgctg gctctggaaa aactggaaaa ggttcccgca gacaacaatg
     3961 ccgatgagaa gatttgtcag gcaaagatcg acgcgattga agaagttgtc gtacagcggg
     4021 aggaaaccaa tcgttctctc attacgctgt atgaaaagct gtacgatgcg atccgttaag
     4081 gcaataccgc acaaagcttg tgcgtaaggt gcgttgtcag atttttcggc agattgccta
     4141 gaaattccgc aggcatactg ccgtatgtca agggatttct gggcaatatg acggaaaatc
     4201 tgcacgcaga ttgcgtacaa agccatcagg cgggctttgt gctgtattgc cttaaggaaa
     4261 taccgcactg acaaagccgg caggctggct ttatgcggtg tcgcctttac acgcgatcac
     4321 tataccaaaa caaaaaccgt ttcgcttccg gaactctccg acggcgaaac ggtttttttg
     4381 ttttcttaag gaaacactta ttccgatttt gcggcggtgt ctgccggctt tttgcccaca
     4441 gagaagcaca cccagaaaat cactgccaac agcagcacgg cgaacgccat ccagccgaga
     4501 agcacgcccg taccctttcc ggcgaacatg tcataatagc ccttgaggta gatcaggcaa
     4561 atcaaggtcg gcaccacgta tgccatatac ccccgcagcc atgccggcag cttcgtgcct
     4621 gtaccctcgt tgacttctgc cagaaacttt tcccagcccc agccgttttt ccggacgcag
     4681 aacagcacat agatcagcga acccagcggc aggatattat aggaaaccaa aaagtcctcc
     4741 agatccatca cgctggagcc gtccccgaag ggctggaagc cggacagcag attgaagccc
     4801 agaattgccg gcagggacag cacgaagatc agcaccagat tcaccagcag cgatttcttt
     4861 cgtgtccagc tgcccagttc cacggtcatg gtgatgatgt tttcaaacac ggcaatgacg
     4921 gtggagagag cggcaaagga catgaacacg aagaagcagc agccccagat gcggccgccc
     4981 accatgtgtt ggaacacgtt cggcagggtg atgaacagca gggacggacc ggcatccggc
     5041 tgtacgccat aggcaaagca cgccggaatg atgatcagac cagccataac ggcaacgaag
     5101 gtatccagaa ttaccacgtt gatcgcttcg ccggcgatac gccgaccttt tttcagatag
     5161 ctgccgaaga tctccattgc accgatgccc acgctcaggg tgaagaacgc gtgcgtcatg
     5221 gcggcgaatg ccacagaacc gtagccgttt tctttcaggc gggagaagct ggggacgagg
     5281 aaatatttca gaccctcgcc tgctcctttc agccggagtg agttcaccac cagtaccagc
     5341 atcagcacaa tgagcagcat catcatgact ttggtgatct tttcaatgcc gttttgcagt
     5401 cccagagcac agatgccgat acagagaaca atgacaatac cggcaaacag gatcatcagt
     5461 gccggactgt ccagcatgcg ggagaaaaag ccggacgttt cttcggcgga aacggcggtg
     5521 atctttcccg aaccgtacag gaacgcgtag tagagcatcc agccggcaac catggtatag
     5581 aacatcatca gcagataatt tccggcaatg ccggcgtatt tggtgatgtg ccagcggctg
     5641 cccggctgtt ccagaatgtt cagtgcctgt gaaatgctgc ggcggctgcc ccgtccaatg
     5701 gcaaattcgc agatgacaat gggcagtccc agcagaagca gacacagcag gtagatgagc
     5761 agaaatgctc cgccgccgtt ttcgccgcag atgtacggga atttccagac gttgcccagt
     5821 cccacggcac agccggcaga taccagaata aagcccagcc gcgagtggaa tgtcgctcgt
     5881 tttttggatg atgtactcat atcagttctc ctttgtaatt ccgcagttgc agaaaatgtg
     5941 attgttatat tatacaacaa gattgcgttt ttgtcaattg cgacgaaagt ggtaggtgtt
     6001 ttggcaggtg gggtggtgcg gacacggcgt tccatgttcc cccattttac gaaaaaacgc
     6061 accccacaaa cgtgagatgc gttttccggc agagaacatc tgccgagtta cttcttccac
     6121 atcagcgtgc cgtcctctgc gtagacctcc tgcacctgat cgggatcgac cggttccaga
     6181 aaactcatgt acaaagtcac acgtccctcg gcatacgcct gatcgacata gacatagggc
     6241 acatgtccga cgcggtagaa cagattatcc agatctttcc gttccttttc cacctgtctg
     6301 gtcaagccgt ccggtgtccg gacataaccg gtacaagagc cattcagaat atcgtgttca
     6361 ttcactgtca catagccaaa cacccgtggc atggggtctg taggtttcca tgtaaaggaa
     6421 acaccccacg gagaaagttc catggtcatc ggcgtatctt ttcccgtttc agagtccgta
     6481 aatgtaaaac tgtctgtcac ggtctttgtc atgggatagt ccagatcgaa ttccatgctg
     6541 ataccgccct cggtcagaat gtccgacttt aacaggtata cccgctcatc atcgttctgc
     6601 cacttttcac cgctgagatt cccgtgttgt attgcactca tttggtctaa cagctcgaaa
     6661 ttttctgaaa ctttctgcca gtcctccgcg gacaagggct ttttggtatt gatatccgcc
     6721 atagaatagc ccagccagtt ccgaatatcc cacatatcat acgtttcgcc ccaatcccat
     6781 gtcagatagg catcaggtgc attggagccg gtcagaaatt tctcgatctc catagaaact
     6841 ttcgggtggc tcttaaaatc gatctgatcg agttcgttat ggatcgtcac ataccaatgc
     6901 ccgctgtcag aagcatcata aatatagtct atcttgctgg agccaccggt ggcaacatcg
     6961 ctttctatcc aaggaaattc cccgaaaaac cgcggtgttt ccggcagctg aaaaccgttg
     7021 acaccggaaa attccagaac gaagttgacg gtatgctcat ctccaaatac gcccagcacc
     7081 tgacagtcga tgtcctcgca tgtgtccgtc attttcactt caggaacggc atacagttct
     7141 tccagcgtgg acggatacga aacgtctttt ccaaacacca gctgcatcag ctgggaaaag
     7201 ccgccggttg ccgctgccgc ggtgatcgca ctgccgctga ccagcactgc cgccgcgatc
     7261 agcaccgccg gaatccgtcc cattttccag tgcggagcgt gacttcgtgc cggcacgccg
     7321 gctgcggtac attctggttt tcacggtgga ttctttcatg tgcatttcgc cggcaatttc
     7381 ccgcacggac tggcggtaga aatagaaccg cagcagcagt tcccgatcct gttccggcag
     7441 ctgtgccagc atttcctgta caatgcccgc cagttccgcc tgctctgcct gtgccgaaac
     7501 atcggtatca tcggagatca ctacgtcatc aatgggcatg gtttcggggc ggtttttccg
     7561 cagccagttg taccccttgg aacgggcaat gctggcgaga tagccggtga ggtgttccgt
     7621 ccgcatctcg ggagcgtggt gccacagggc aaggaacacg tctgcggtga gttcctccaa
     7681 atcctcctgt gttgtctgtc cgcgggtgac attccgcagg attgttccca cataggcggt
     7741 atagcgtttc atcacccgtt ccagcccgcg gcggttctgt tgtttcagct gttccagcag
     7801 ccgtgcatct tttccggtgt cctccggagc cgccgccgac agaagcagtt ctgccggaca
     7861 gctttcccat gttgttgcat gcagcatcgt gccgcctcct ttccggagag catatgctta
     7921 gggaaacact cttgcagtgt ttccttagat atgctctgtg tggtttcata tagatagaca
     7981 ccggtctgtc ggaattggtt tcactttcgg ggaaattttt tgcggtgccg ctttcgtgaa
     8041 aacccatgag aaaacggtaa gggcatagaa gaaaattaca gcaagaaaca tacatttcaa
     8101 tttttcatac cccaaacaaa aagcggacag cacttacact gtccgcttct tctttttaca
     8161 gctgtcgaat ctccgcatcc gggatttcct cgtcctccgg tttttccggc ggcacgatgt
     8221 cccgtatctc gaaccagaac gtagagccct cgcccagctt actgttgaca ccataattat
     8281 agttatgcat cttcaggatt tctttcacga tagacaagcc cagtccggtg cctaccactt
     8341 cccgcttatg gttctcggaa cggtagtact tatcgaagat cagcttgatc ttgtcctcct
     8401 cgatgccgtc gccggtgtcc cgcacttcaa ttcgcacgca ctggggcaga ttccgctgcg
     8461 tgacaaacac ctgtttgtcc tccttggagg tgtaattgat cgcgttattg atcaggtttc
     8521 cgatcacgcg gtcgatcttg ctctcgtcgc agcgtaccat gcagggctcg tccagcgtca
     8581 gatggatctg atagcccata cgctccgaca cgccctcata gcggtgcacg atctccgcca
     8641 tgcactgggc gatgtcaaac gtgctgtaat ccagcttctg tgctccgctt tccagcttgg
     8701 acaagtccag catatcgttg acgatctgcg tcagccggtc ggtttcctcg atgatgatgt
     8761 tcaggtgctg ttcccgcttc accggattgt tccccgacag gtcacgaatc atttccgcat
     8821 aggctttcat catggtcagg ggcgttcgca ggtcgtggga aacgttggcg atcagatccc
     8881 gctgcatctg gtctgtcttg gaaagctcat gtgccgcata ggtcagcgtt tccgccagat
     8941 cgtctacttc ggcatagccg ccgccctcga acttcaccgc ataatccccg tgtgccaacc
     9001 gcttggcaga ctgggtaata cgggtaatgg gagtggcgat gtggttcgcc gcatagaacg
     9061 agatcaggaa cccgaagaac accagcataa tggtgatcat catggtctgc cgcttcagaa
     9121 tggacacggt ggattccacc ggcaccagcg gtgaattcag cagcagatag cccagcacgc
     9181 tgccgtcgtc cttgctgtac atggtacagc cgtagaccag catttcattt tgcagtgtcg
     9241 ggttggacac ccgccggcag atcgtgccgg tgctgctgtt ctggaggtcg atgagataga
     9301 acagcgttcc gctttctttg ccgtgcagca ggcacttgcc gttatacaca cagttatagt
     9361 acacttccct gccgttggaa ttgagcagtt caatgcacat ttcattttgc agcgagatct
     9421 cctgtgcatc gtcagaaaag tcctcactgc catagtccga caccagctgc tgtgccagtt
     9481 ttgccgtgtc gcggatcttc atggactcat agaaccattc cagaaagatg atctgaaaca
     9541 gccacatcag cagaaatacc acgcacacaa acagcataaa aaacagccag atctttacgc
     9601 cgatgcggag attgcgttca ccgagatgat gccggtgatg cgtcattttc cggatcaaat
     9661 ttatatccca ttcctctcaa ggtcacaata tctgaacgat acggtccaag attgccgcga
     9721 agcatcttga tatgcgtatc cacagtacgg tcgtccccga agaaatcgta tccccagact
     9781 tcttccagca gcttatctct ggaaagagcc agtcccttat ttttcaccag atagaacagc
     9841 aggtcgtatt cttttggtgt catggacgca cgcacgccgt ccacatacac ctcccgtccg
     9901 gcaaggtcaa tctccagccc gccgaatttc agccgctgct gggtatcgcc gccgccctgc
     9961 tttgccgcat ggctccgtgc cagaactgca cgcacccgtg ccatgagttc tctgggcgaa
    10021 aagggcttta ccacatagtc gtcaatgccg gtttcaaaac cgaacagctt gtcgtattcc
    10081 tcgccgcggg cggacagcat gataatggga acatctttct gcttccggat ctccttgcag
    10141 gcggaatagc cgtccagacg gggcatcatc acgtccatga taatgatgtc ataatcattt
    10201 tcccgacaga gtgccaccgc ctccatgccg tcccgtgctt cggtcacttc gaactcctcg
    10261 aactctgcgt actcccgcac tacggcacga attttttctt catcgtctac gatcaacatt
    10321 ttgctcatgg gttccacctc cctggaaagg tatggatcag cctcagctgc tgcgtaacat
    10381 tttcaaaaac gccgcacagt actcttactg attccgtttt ttcagtgccg caccgataaa
    10441 ggaagcgaac agcttctggg gcttgttcgg gcgggacttg aattccggat ggaactgtac
    10501 gccgataaac cacgggtgat ccggcaggga aacgatctct accagacgtt cgtccggaga
    10561 aagtccggcg atctgcaagc ccttttcggt cagctgcttg cggaagttat tgttgaattc
    10621 aaaccggtgg cggtgacgct catagatcag gtcttcgccg tagatctggt gtaccagact
    10681 gcccgctgtc agcttgcacg gatacagtcc cagacgcatg gtgccgccca tgttggtaat
    10741 gtctttctgg tcttccatga tgtgaatgac actgtgtgca ccgtccggct caaactcgga
    10801 ggagtttgcg tccggaaaac cagccacgtt gcgtgcaaat tccaccactg ccatctgcat
    10861 gcccagacag atgccgaaca tgggcacctt gttcagacgg gcatagcgaa tggcttcgat
    10921 catgccctcg ataccgcggt cgccgaagcc gccgggaacg ataatgccat cgcagtcctt
    10981 cagcatttcc ggagccgttt cccgtgtgat cttctcggag tcgatccact ggatcttcac
    11041 tctggcatca ttgacaatac cgccgtggtg cagagcctct gccacagaca gatatgcgtc
    11101 ctgcaatgcc acgtacttgc ccaccagacc aatggtcacg tccttgtcag cacgcttctg
    11161 ccgttcggtc atggcagtcc attcggtgag gtcgggttcc cgattctcca gacccaggtg
    11221 gtggcagacg ctgtctgtca gaccttcgtt ctccagcatc atgggcactt cgtacaggga
    11281 cggtgccgtc atgttctgga taatgtcttc ttcccgtaca ttacagaaca tgctgatctt
    11341 ccgcttcata tcctccggaa tctccagctc cgtccggcag actaccacgt ggggctgaat
    11401 gccgatagac agcagttcct tgacggagtg ctgtgtgggc ttggatttca gttcattaga
    11461 gccggcaata tacggcacca gtgtcacgtg aatgtacatg gcattgcctc tgcccacttc
    11521 ggtgacgaac tgacgaatcg cttccagaaa cggtgtgctc tcgatgtcgc ctacggtacc
    11581 gccgatctct gtgatgacaa catctgcgtt ggattccttg ccgacacgat agatgcggtc
    11641 cttgatctcg ttggtgatgt gcgggatcac ctgcacagtg ccgcccagat agtcgccgcg
    11701 gcgttccttg ttcagaacgg tccagtacac tttaccggtg gtaacattgt tgttgatcga
    11761 caggctctcg tcgatgaaac gctcgtagtg accgagatcc agatcggttt ctgcaccgtc
    11821 gtccgttacg aatacttcac cgtgctggta cgggctcatg gtacccggat ccacgttgat
    11881 atacgggtca aatttctgga tcgtgacctt atagccgcgg gctttcagca gacgacccag
    11941 cgaagccgcg gtgattccct tacccagtcc ggacacaacg ccgccggtga caaatacata
    12001 ttttggcata tttaataatc ctcccgtttg ttcgggcagt cctgtacaaa gactgtgtgc
    12061 aaagccgtca ggcgggcttt gtgcggtgct gcccttaggt tctttttcag agcctgcatc
    12121 atgcaaacat attgattcat cttattatac cgtattccgc caaaaaaagc aagcccttat
    12181 ttttgaaatc catggaaatc aattttcata atagcctccc ctgaaagggg agggggaccg
    12241 ccgttaggcg gtggagaggt tctttcgtaa aatcttttta tgacgttttt tgcataagtg
    12301 gaaacccctc agtcagtgct acgcactgcc agctcagcac aaggca
//
LOCUS       DAWBGR010000064        12240 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1561_length_12240_cov_4.777185, whole genome shotgun sequence.
ACCESSION   DAWBGR010000064 DAWBGR010000000
VERSION     DAWBGR010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12240)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1561_length_12240_cov_4.777185"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2791 a         3213 c         3430 g         2806 t
ORIGIN      
        1 tgcccagaaa tcccttgcaa tacgactgcg ttcgaggcag gattctgctc gagaacaaag
       61 gagtgtcgca ctaagatgaa ttgcgaccat acagtatgcc tgcggaattt ctaggcaatc
      121 tgccgaaaaa atctgactac gcatcttacg cacaagcttt atgcagcgtt tccctaagct
      181 ttggaaaatc gatggcggca gacttatgcc ggcaggcgga accgttttgc ggcgacgctg
      241 caaatgccga atgcgggaat gcacagcaaa aaccacagtc ccgaaaaggg caggcagatc
      301 tgtccgtaga ggttcagcgg catgctgctg tagtcccaga cgttccagcc catgaccaga
      361 ttgtccgcca ccccaacggt gaattccagt gccgtgatca gcagtgctcc gcaaaggcat
      421 tgcagcagca gcgttttcgg cggtgctgcc gcgtggagca gatacagccc caccgcagcc
      481 gtaccgccgg tgagcgtcat ggtccagtgg gtgtacccgc ggcagacgat ctccagcaga
      541 ctgtagacaa acgcccccag caggaagaac cagcagagtt cgtagaatcg gatatgcagc
      601 ttcaaaaaaa tcacttcctg tttcgtgtgt atgcataaac gtagtctgtg cagaatggga
      661 gaaaatatgc ggctggagtt tccagtccaa agcgatctgt tcaagtttta ttttttggag
      721 gtatcaatat gcgaaaaagt ggtattttga tgcacatttc cagtttgccg tcagaattcg
      781 gcatcggaaa gctgggcgat tctgcctata tgtttgccaa tttcctgcgg gactgtggtg
      841 tgcaggtgtg gcagatcctg ccgctgtcgc cgaccagcta cggcgattcc ccgtaccagt
      901 ccttttccgt ccacgccgga aatccctatt tcattgattt ccagcggctg gaaaagcagg
      961 gctatctggc accgccggat tacgaggaca ttgtctgggg cgaagacccg cgtcgggtag
     1021 actatgcccg catatataat tattgtttcc acgtgctgcg gacggcgttt tcccgtttcc
     1081 ggaaagagga taaagcctat ctggaattct gcgaagccca gaagtactgg ctgccggact
     1141 atgccctgtt tatggcgttg aaagatgccc atgacggcaa gccgtggtac cagtgggaaa
     1201 agccgctggc aatgcgggaa ccgaaggcac tggaaaaggc aaaggaaacc tatgcggaac
     1261 agatcagctt ctacactgtg ctgcaattct ggttctatca gcagtggggt cagctgaaag
     1321 cgtactgcaa ccagaatggc atttccattg tcggcgatat tcccatttat gtggcatatg
     1381 acagcgttga tgtgtgggtg aatccggaac tgttcctgct ggacaagacc agaacgccca
     1441 tcgatgtggc aggctgtccg ccggacgtgt tctcacctac cggtcagctg tggggcaatc
     1501 cgctgtatga ctggaagtat catcagaaaa ccggttttgc atggtggatc cagcggctga
     1561 aaagtgcatc cacactgtac aacaccgtgc gtatcgacca cttccgcggc ttcgaaagct
     1621 tttatgcgat cccctacggc aaaaaaactg ccgaggtggg cgagtggcgt aagggcccgg
     1681 gcatggctct gttccaagct gtcaaggaag cactgggcga cctgtcgatc attgcggaag
     1741 atctgggctt tgtgacaccg gaagtgcgga agctgctgga ggattccggc tatccgggca
     1801 tgaaagtatt gcagtttgcc tttgacgatg atcccaagag cacctatctg ccccagaact
     1861 acaccacgac caactgcgtt gcctacacgg gcacgcatga taatatgacg ctccgcggct
     1921 ggatccacgg ctcgccggca aagaccattg cctttgcgaa gcggtatctc cactgccgga
     1981 gtgccaacga cctgccggct gccattctgc ggctgacatg gagcagcacg gcaaacctcg
     2041 ccgtggcaca gatgcaggac tttctggacg caggtccgga gggccgcatg aatacgccgt
     2101 ccacgaccgg cggcaactgg cagtatcgga cactgaccag cgatttcaac cccagactgg
     2161 caaagcggat ctaccagctg aatgaactgt tcaaccgtct gccggaagaa tccctgcaaa
     2221 agccggtgtc caaaaagcgg cagaaagcag cggcaaaagc agcagccaag gcggctgccg
     2281 gaacgaaacc tatggagaaa aaggagaaga agtcatgatg aagaaagaag cattgaaaac
     2341 cggtatcctg aacgccctcc acggcgtaga cccgaaaacc gctaccccgc agcagctgca
     2401 tcaggcactg ggcgaaactg tcatggaagc cattgcaccg gattggaaaa agagcatgga
     2461 cgctcacgaa cacagccgcc gtgcctgcta tttctctatg gaatttctgg tgggacgtgc
     2521 cgtttacaac aacctgctgg tgctggacgt gatgcaggag gcagaggaag cctttgcaga
     2581 actgggcgtt tccctgtctg atctggaagt gatcgaagat gcagcactgg gcaacggcgg
     2641 tctgggcaga ctggctgcat gctttttgga cagtgcggca acactggatc tgccgctgga
     2701 cggctatggc attcgctata agtacggtct gttcaagcag tacttcgagg acggctgcca
     2761 gaaggaagtg ccggacgatt ggatgcgtta cggtgatgcg tggtctgtcc gtcgggaaga
     2821 agatgccgtg ctggtagaat ttgcaggaca gaccgttcgt gccgtgccct atgacatgcc
     2881 ggtcattggc tgcaagacca agcacatcgg caatctgcgg ctgtggcagg cagagccggt
     2941 gcacaccttt gacttcgacc tgttcaacca gcagaagtat ctggaagctg cggcagaaac
     3001 cgtgtatgca gaggacattt cacgggtgct gtaccccagt gatgacacat gggagggtaa
     3061 gaagctgcgt ctgaaacagc agtatttctt ctgtgccgca tctttgcagg acattctgaa
     3121 aaagcataag gctgcctatg gcacgctgga caatctggca gacaagctgg cgatccagct
     3181 gaacgatacc catccggtca tctctgtacc ggaactggtg cgtcagctga tgctgccgga
     3241 aaacggctat acattcgagc gggcgttcgg catggcacgg cagatcttcc actacaccaa
     3301 tcataccgtt atgccggaag cactggaaaa gtgggactgc aatctggtgc agcagctgct
     3361 gccggcagtc tatgacatca ttctgcgaat cgaggaacag ttcatgaccg agatgtacca
     3421 gaagggtgtg gacaaggcac aggcaaaccg catgaagctg gtgcaggacg gcatggtgca
     3481 catggcacgg atagcagtgt atgcttcggc acacaccaac ggcgttgcgg caatccatac
     3541 cgaaattctg aaggattccg tgctgaaaga ctggtatcag gtttatccgg aacggttcca
     3601 gaacaaaacc aacggcatca cccagcgtcg ctggctggct ctgtgcaatc cggaactgtc
     3661 cggtctgctg accgaactgc tgggttctga tgactggaag attcacctgg acgacctgaa
     3721 gcagctggaa cgctacgccg acgataccgc tgtactggag cgttttctgg caatcaagca
     3781 gaccaagaag gaacagcttg ctgcatatat cgcagaaaaa gagggcgtac agatcgaccc
     3841 gcagtcgatt ttcgacattc agatcaagcg gctgcatgaa tataagcgtc agctgctgaa
     3901 tgcgttctcc attctgtatc tgtacttcgg actgaaagac ggcagcattg cggacatcac
     3961 accggtgaca ttcctgttcg gtgcaaagtc tgctccgggc tatcgccgtg ccaaggcgat
     4021 catcaagttt atccacgaag tggcaaagct ggtggaagca gatccgctgg tttcgcagaa
     4081 gatcaaggtg gtattcgttt ccaactacaa cgtttcctat gcggaaaagc tggtagctgc
     4141 ggcagacgtt tccgagcaga tctccaccgc cggcacagag gcttccggca ccggcaacat
     4201 gaagctgatg ctcaacggtg cagtgacact gggtacctat gacggtgcca acatcgagat
     4261 cgtggaagaa gccggcgagg aaaacaacta catctttggt gcaaaggtag aggaactgga
     4321 acagatcatg ccgacctacg acagccggaa gctgttctcc gagaacgaaa agattcgccg
     4381 cgtggtagaa accctgatcg acggcacctg ctgcgacggc ggcagcggcg atttccgcga
     4441 actgtactat tctctgctgg acggtgccag ctggcatgca ccggataact actatctgct
     4501 gggcgatctg gaatcctatg tggctgcaaa gcttcgctgc aacggcgact atacagatcg
     4561 tatggcattt gccaagaagc agtggttgaa tatctgcaac gctggcaagt tcagttccga
     4621 ccgcaccatt gcagactatg cagaaaacat ctggaacatt caggcagtca cagtagactg
     4681 atcggtacgg cattctggga ggtgctgtaa atgggcggcg tgatctcatt tttcattttc
     4741 ttcctgctgg gcattctgga taccgttttt gcagtgaagc tgaatctgtc gccgtggaag
     4801 ttcctgctgg cgggaaaccc gcggcttccg gacggcacgc ggctgtatga tatggaatgc
     4861 tttcagcggg cggcgggcat gctctgtgcc atttatgccg gtgtcttcta catgacggca
     4921 ctgtttgttc tgctgacaca gattctgtgc gtgatgccca atgcactgat ggcaggcatg
     4981 ctgtatatac acggggcagt gctgacaatg acagcattcg gtctgctggc gtatctggac
     5041 agctcttgtc aggtgcagcc gcagacggaa aaactgtcag agtaaaatca catcaaaaaa
     5101 caaaaaacgc tatggtacgc aaagtaccat agcgtttttg ttttagggca ataccgtaca
     5161 aaaatctgca cgcagattgc gtgcaaagcc gtcaggcggg ctttaaaagt aaactcaatc
     5221 cattttcttg ccgcaggagc cgcagaactg gctgtccagc gggttcatgg caccgcagtg
     5281 cgggcagaac ttcacggtag aagaatcatc ggtcttggga gcatggcttt cgccggacat
     5341 ttcggtgtag cggagttccg gttcagccgg tgcattgtcc ggagcctgcg gagcaggtgc
     5401 ttccggtgcg ggagcattgg ggacaggtgc actgacggca gcagcctttt tctttttgga
     5461 cttgactgcc gcgttggtgc agatgccgat cactgtaaag agtacgccat atgccgccgg
     5521 aatcagcgtt gccagagcga ccggtttcag cagtatcggc agccctgcct tggagaagat
     5581 cagtgcatct ggtatgtgga acgcctccac cagcagcagg gtgacaccac ttgccgccag
     5641 taccagcaga ccgggaattg ccactgccag accgtaggcg gtgaacattc tgcccttgtg
     5701 ccagtgcttt ttcactgcca gcaccagcca cagcacgaag catgccactg tcagcacacc
     5761 aaaggcgatc atgccggaca gattgcacag ctgccgtgtc aggaatttgc cgaagccgga
     5821 accgatcgtg tgctgcagca tgctgttcca gccgttgagg tcgtcgccca tggtccagtt
     5881 cagggatttc cggtcagctt ccgcaaagac catatgggtt tcctttgtca gcttgctgcc
     5941 cagttcctcc tgcaacaagt ccgccacatc gtccggctgt aactgcggca gttcggagtt
     6001 gtcgccggtc atgtatgtac tcaggtcgtc cagatagtca cagaggaact gattgcactc
     6061 cggcatggtc accagctttt cggcatattc ctctgtcaga ttgggagcgt cccagaagta
     6121 ccagtcatac aggcacttgc caatgagctg ctcctttccg tggtcggaaa gctgtatctg
     6181 ggacaagtcc attttcgcta ttgccgcgga cagtgttttc tggggcagta cgctgtttaa
     6241 gatcgaactg cacaaaaagc agatgccgaa gagcagcgtc agcagaaaca gcaggatcga
     6301 accgaaaacg gctccggccc gtgtgggctg cttctgtttc ttttcctttt tgggaactgc
     6361 ctcggcttcg gcagtcgtgt tggatctttc ttttgccatg tccattcctc ctgttcggca
     6421 gcaccgcata agcagtacta tggaattgtt ttgtttctat gaaaaaatta tagcatacaa
     6481 aactgcctcg aatgtgtatt ttctgaaaat tttaggacaa taccgcacaa agtcgccaga
     6541 cgctctttgt gcggtattgc cttagcatca tgcaaacatc tcccagccgc gaatccgttc
     6601 ccgcatctcg tcaatgccca gcaccccgca ggcgaacccc tttttcacca gttcttccgg
     6661 caggtactcg tcgtactttt caaagacagg tacttcgttg gggtagacca gttccatcgg
     6721 atccagcggc ttgcgttcct gttccagcag cacgcggaaa aaagtccgct tgtccgcctg
     6781 cattttgaac tggataaaat agggacggga gggtgccagt ccggtcagct tcagagcacc
     6841 ggcacagcgg cacttgagaa atcgttccat tgccagaaag gcatacgatc ccagccacgg
     6901 aaacaggcac cacatgtcac cgcccagaca gatcagcggc tcctctgtga caccggagtt
     6961 ccgagccaca tgccgtgcca gctgtaggcg ggatacggca tttttcatca gatagggata
     7021 ggatttctgt tcctgcaata ccagccgcat gcgttccagt accttcgtgt gaatatcgcc
     7081 ggggcattcg ccgaagtagg cgggaacttt ccccttgacc tgttcgcagt acaccgtgtg
     7141 ccgctggtga tccacttctt ccaccaccca gacgtgaccg gcaatggcga tcttttctcc
     7201 agccggcggg ggctgtacaa tggtgcccag ttcctgcgag ttccagcgga cagtgtattc
     7261 ctcgttttcc tgaaagacgg cgtagaactt gtaggaactg gtgagccgtt cgccggcaat
     7321 gcccacgatc aggccgccgg tttccgtgcg ttctatgtgg tcgatttcca gcaggtgccg
     7381 cagcagcagc ttgaagtcct cctgtgaaac gcggtggaaa tagctgagtg tcagtacgcg
     7441 ggaagccaga gccgccggtg tcagctcacc gcaggaggcg agcgtgcaca tggtctggtg
     7501 atacagcagg ctgtagggca gccgatccag tcggggcggt tctacccagc gttcctccag
     7561 atagacctgt accaaggcga tgccctgtaa cagtttccat gggatcgtgg tgggcagcag
     7621 ggcacgtggc tccggcggtt cctcccgcat gacaaaccac atctccggcg gcaggtcacg
     7681 ccgtccggta cgtcccatac gctgcaaaaa agaggacacc gtaaaaggag catcgatctg
     7741 gaacgcccgt tccagcctgc cgatgtcaat gcccagttcc agcgtggcag tggtgacggt
     7801 cgtcaggaag gaatcctcgt ctttcattgc ctcctctgct gtttcccgat aggcagagga
     7861 cagattcccg tggtgaatga gaaagcgatc cggctcgtgt ttgacctcgc agtattgccg
     7921 gagcgtggtg gtgacggctt cgcactcctc gcgggagttg acgaagatca gacacttctt
     7981 tccgcgggtg tgctcgaaaa tataccccat gccggggtct gcatagtccg gagccgtgtc
     8041 ggttttcggg gcgagttccg gcaatgcctg tgccggcgga ttgtcctcgg cagcctgccg
     8101 atcctgcaca tagaaatgtt ccatagacag ccgccagcga acgcccttgg actggatacg
     8161 gggaatcacc gttcctctgc cggtgccggc ggcgaggaat ttgccggtgg cttccagatc
     8221 gccaatggtg gcagaaagcc cgatgcggcg gggattgaca ccagccatgc ggcagagccg
     8281 ttcgatcagg caaatggtct gcccgccgcg atcgccacgc atcagggagt gcacctcgtc
     8341 aatgacgata aaccgcagat cgtggaacag cttggtaatg gcagcgtgct tgtgcagcag
     8401 cattgcctcc agcgactccg gcgtaatttg taagatgccg gaggggtgct tcatcatctt
     8461 ggctttgtgg gactgggaaa cgtcaccgtg ccagtgccag acgggaatgt ctgcttcggc
     8521 acagaggtcg ttcagccgga gaaactggtc gttgatgagg gctttcagcg ggccaatgta
     8581 aattgcccct accgaagtgg gcgggttctc ggaaaacagg gtgagaatgg gaaaaaatgc
     8641 cgcctcggtc ttgccggagg ctgtggaggc ggacagcagg acattttcgt ctgtgtggaa
     8701 aatggcatcg cccgcagcca cctgaatggc acggagcgat tcccagtcgt tctggtagat
     8761 gaaatcctga ataaacggag catagcgttc aaaaatagac atggaaaaac ctcttgggga
     8821 aacacggaat aaagcttgtg cggtattgcc ttaaatgtca aactctgcaa attccggtgc
     8881 aacgctctga tcgctctccg gctgtgccgc ggcgtaggag aactggtcgg actgtaagag
     8941 ttctgccaca tgcatctgcg ggttctggaa aatgatgtcc agcagttcga taaagtcccg
     9001 aattacctcg cggggcgtga tgtgagaatc tgccccgatc ctgccgaatt cgatctgcaa
     9061 aaacaggata cgatcttcct gtgtcagcgt aggcgtatac ccgaacagct gggcatgaat
     9121 ttccgtcagc ttttctgtca gcaccagcaa ttcctcattg gtcagggcgt tcaggtggat
     9181 caccggagcc agcatatccc gcatgccctc ctgtgcaaaa cgtccctctg ccaggcggga
     9241 acggagagct tcatagctgt acacgccgcg gcgtgtgtcc tcgatgcact gaggtgtgcc
     9301 gcccatgatg atgcccagat actgtgcctt gccctgcatg gtgtcgttgt acatggtgag
     9361 gatcttttca taattgtact ggcgggtaat gctgtgggga actttgtaga tgttcaccag
     9421 ttcgtcgatc agcaccagca tgccgctgta gccggctttt ttcaggaaca tggcgaacag
     9481 cttgatatac tcgtaccagt cgtcatcggt gataatgatg ttcacgccca gttccgccct
     9541 ggcttcggtc ttggtattgt attccccgcg gaaccacttg accacctttg ccttgcattc
     9601 gtcgtcgcct tcccggtagg cgttgtagta cagcgtcagc agtctggcaa agtcgaaccc
     9661 gtgcaccatt tcatgcagtg ctccgatgac ttcgtagatt tttcgttcca cggctttccg
     9721 gaactggggg tcgttggtat ccagaccgga ttccgcggca gtttcgctct ggacgttgct
     9781 gatccagcga tccagaatca gcgtcagtgc accgccgtca ggacgtgtct tggtggacat
     9841 gttccgaatc agctctttat aggttgccag tccctgcccc ttggtgccct gtaaccgccg
     9901 ttcgggggag aggtcggcat ctacgacaac gaaatttctg tccatgacat agctgcgaat
     9961 ggtctggagc agaaagcttt tgccgctgcc gtacttgccc acgataaacc ggaacgatgc
    10021 tccgccgtct gcgataatgt ccacatcgtg gagcagggcg ttgatctcct gttctctgcc
    10081 tacggtgatg tagggcagac cgattcgcgg cacaacgccg cctttcaggg aattgatgat
    10141 cgccgaagcg attcgcttgg gtattttcat gataacgatc ctttctgaaa cggactgtat
    10201 gggcatattg acaatgaaac aagccttccc tgaaagggaa gggcatggaa ttttgatgta
    10261 ttagttctaa ggaagaacct ctctcacccc agcaatgccc gcagctcctc cacataatcc
    10321 tcgatcagtt ccggctggtc gccgtcgaat aaaatgaccg tgtcgccgaa gtcatcgaac
    10381 aacgtttcgt tgatgtggtc taccagcacc gagagcatca cgctgccttg ccggagcagg
    10441 tctgcatagg gctggtgttc cagcagacag tgcaggaacg cccgttcggt ttcgctgagc
    10501 cgtgtatctg cggcaggtgt ttccggtgca gccggttctg ccggtgttgt ttccggaacc
    10561 ggcagttccg gagcaggctt tggagcagct tgcagttccg gttcttcctc ggtgtccacc
    10621 agcagcttgt cccgtgtgac ctctgccgcc tgccggatcc ctgccagacg ggacaggtcg
    10681 aattccactt tcggaacggc gttctttttc cgttcgtcca gaaatgccgc cagagttttg
    10741 tccagcacct gctggagata tttcggcagt tccgtttctt ttatggggtg gggatactgt
    10801 actagctgcc gcagcttctg gtcaatggtc ttgacaaaac agcccagccg tttctttccc
    10861 ttttccgcat cgaaataggc ttcgcatgtc cacttgccgc tgcggcagcg gtaaatatga
    10921 tacggcgata aaacatagtc gcagtttctc tccggatcgg tttcaaaaaa cgtggcatag
    10981 ttgaacatgt ggtaggggag cagccccttt tttccgaaca ggtattccag atagtcgctc
    11041 tttttccggt tccggaagta gtcgcacatc tggcggaaac agtagggcac tgcctgttcg
    11101 atcagttccg tctgctctct gtaaaacttg gaacgctgga gcccgtagcc ggacaagtcg
    11161 gaaatcgcct gaaacagttc tgcatctgtg tgtgtttctg cttcctgcac cgtctgaaag
    11221 cttttgtcga accgtgcttc cgcggtgtag tcctcagaga gttccggcaa aagagcctga
    11281 tccagtccgt agtaaatgat gtagtcatgg agccatatcg agaaataggg gaagcggcat
    11341 tccggcgaaa ctgcggtata gtcctgccgg aactgctgta acatgcggaa tccggtttcc
    11401 ggatctggta cgccgatctg gtgcagcagc tcatagcagt agagttccgc gtagctggga
    11461 gaagtgcttt ccacaatgcc ctgccgcact ttggttcgcc atgtgaaata ggtgcgaagc
    11521 tgtcggtcgt tcatgtcccg ataacaggga tagcaggagg aaaaagaagc ctgttcggaa
    11581 aaatcgtcgg tgaaatcttc cagcagcttt gcctgctgat agaacagctg tctgtaacta
    11641 taatggtatt ctaccggatt ccgcatcagt cgcttggctt ccaccagcgg tctgggcaga
    11701 agccgctgca tttgtgatgc cgtctgtagg atcggctcgt cgtgatactc ctgccggagc
    11761 cggtctgcaa catgcttcag cttgggattg tctaaaattt cctgaatcag atcttgtaca
    11821 tcttccagat ttgccatgca gtttcgccct ttcttgatat ggctgtatta ctttcaatat
    11881 accatatttt atcggaaaag tcaaacgtca cagccgccgc aatgggaaat cgctgatgtt
    11941 tctctgaaat tgacatatac tatcgataat gtgccggata cgttctctgc tgatcgcagg
    12001 gacggaaagc ggattctgat aatgattgtt tcggaaaacc ggagagggca cagcgtacca
    12061 gagcatattc ccataaaaag cataagccaa aaagcgttta cacaaaagaa taaaaagagt
    12121 tttggaaaaa ttatagaaaa agaaatcact ttgtggtata ctagaagcgt tcgggcttgt
    12181 gaaagggcaa taccgcacaa agattgtgcg acagatgcgt cgtcaaattt tttgtgaaat
//
LOCUS       DAWBGR010000065        12080 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1592_length_12080_cov_3.169647, whole genome shotgun sequence.
ACCESSION   DAWBGR010000065 DAWBGR010000000
VERSION     DAWBGR010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12080)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1592_length_12080_cov_3.169647"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1940..1949
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3183 a         2639 c         2916 g         3332 t
ORIGIN      
        1 ctctgcggtg tcttaatacg gctttcgata ttgtgaattg ggtttcgctg aaaccgcata
       61 ctgaattcgt tgtcgaaaat attcagctgt gtctttacca gttcaattgc agaatcgtaa
      121 agatgttcca attccaaaaa cgaataaaac acatcttgaa tctgttcttc cttgtgttcc
      181 ggaaccacat cgcgaatggc atccagataa aattccttgt cgtccatata gatgatccct
      241 cctatacaga aaacgcacac aaagaccgtg catttgtgtg cgttttacga aaaatcagag
      301 tgcatacaat gctactctat acgatttgca aatgataatg cctattacgg gagcagacgg
      361 ttgatgtcac gcgggaacat gcttgcctga cgaacatttg acagacccag cagcttcata
      421 accagacgtt ccagaccaat acccagaccg ccgtgcggag gcagaccata tttgtgggct
      481 tccagatagt atttgaagtc ctccggatcc agaccttgtg cgtgcatctt gtccagctgt
      541 tcctgataat cgtggatacg ctgaccgccg ctggtaattt ccagaccgcg gaacaacaag
      601 tcgaacttgt atgccagacg cggatcttct ctggaattca ttgcatagaa cggcggctta
      661 gaggacggga aatgcgtaac aaagataaag tcgctgtcga atgttttctt tgcgtattcg
      721 cagattgcca cttcgtcttc tggttccaga tccagcttgt tcttggaacc tttgttgccc
      781 agaatttcct ttgcttccag cagggtaacg ctgggaatat cccgaattac cggtacatct
      841 gcttccagca gttccagttc aaatgcataa tgctttttca gatattccat gacatatcgc
      901 aggcatgctg tttccattgc cataacgtca tacatgctgt caatgtaacc catttcaaag
      961 tccagaccaa tgtattcgtt gatatgacga gatgttgcgt gcttttctgc acggtatacc
     1021 ggtgcaattt caaatacgcg gtcaaagaat gccacagcag tctgcttgta gaactgcggg
     1081 gactggttca ggaatgccgg tttgtcaaag tattccagat ggaagatatt cgcaccaccc
     1141 tcggcacctt gtgcaacgat cttcggggta tggatctctg tgaagccctg tccaatgagg
     1201 aattcgcgga atccaccgac aacgccttcc tgaatcttga aaatggcacg ttcataaatg
     1261 ttacgcaggg caacagaacg gttgtccaga ttgatgtcca gtgcacagcc cagttttttg
     1321 ttggaaactt tcaacgggta ttcctcgttg cagtttgcaa tgacttccag tgcagacagc
     1381 tgcaactcta cgccgccgac agccttttca ttctttgtaa ccttaccgcg aacccagaca
     1441 aagttgcctt cccgaagtcc ggtgatagaa tccttgcact gttccggtac gtacagcgac
     1501 tgaatcaggt aacgaccggt acgcaaaatc aggaatgcaa aaccactcat ctggcggatc
     1561 ttatgtacgc aggcacgcag agagatttct tctcctacat gatctgctgc atcagagaaa
     1621 tcaaattcgt gcagcagatg atcagtattg atctgtgtga tgccgtcttt cttctccgga
     1681 atatactgat tcggattagt gaacttagtg cattctactg atttaacttc ttctgccgca
     1741 tttgccttgt cagcagattc ttccgcagtg actgccggtt ttgcttctgc cagtttggtt
     1801 tccagtagtt ccttgattgt actgggggtg caaacgccgc gaagggattt ggtgcattgt
     1861 cccatcagga agccgaatac tttcttttca ccagactgat actgtgaaac agcttctgca
     1921 ttctctgaca gaattttgcn nnnnnnnnng ataacctcat ctgccttttt ggtatcgttg
     1981 acaatcagca tgccggattc ttctgcaatg acctttgctg tttttccggt ttcgatcatc
     2041 tgacggagaa tcttcttggc atcgtttttg gatacctgtt cagtatctgc catttttacc
     2101 agttctgcaa attcagcagg ggagaacggc agtgcttcca tagaaacttc gcccagattt
     2161 acacggcgaa gcagttctac aatgataaag ttcgcaatgc tcttaggtgc gttataaact
     2221 gcaacggcat tgtcgtagaa atcggaaacg ctcttttggt tgatcagaat ttgagcatct
     2281 gtcttggaca gaccatagtt tttcatgtag cgttccatac gcttatatgg cagttccggc
     2341 agcatatccc gaatggaatc cagcatttca tctgtaaagt tgacctgcaa aatatccggt
     2401 tccgggaaat accgatagtc atgtgcgttt tccttgcttc tcatggaaga agtttcgccc
     2461 ttgttttcat taaaacgacg ggtttcctgc acaacttttt tgccggcatc cagcagtctg
     2521 gactgccgct tgatctcata attgatggca cgggtaatcg atttcagaga gttcatgttt
     2581 ttgatctctg cacgagtacc cagttcctta tcctccggac gcataataga aacattgaca
     2641 tcgcagcgga gagaaccttc ttccatttta cagtcactga caccagcgta ctgcaacagg
     2701 agcataacct tttcaatgaa agccttcgct tcttctgcgg aagaaatatc cggctctgta
     2761 acgatttcta ccagcgggat accacaacgg ttgtagtctg ccagagaaac ggcgttgaag
     2821 tcatcgtgca ccagttttcc ggcatcttct tccaagtgga tatggttgat tcggatatac
     2881 ttctgtccgt tcggggtgtc aatagcaact ttgccttcca gacaaagcgg ataatacaac
     2941 tgggaaatct gatatgcctt cggcaagtcc ggatagaagt agttctttcg gtcaaaaaca
     3001 gaaaacttgc tgatgtcgca gcccagtgcc ataccagcct ttaccgcata ttctacagca
     3061 gtctggttga tgaccggcag tgtgcccggc ataccggtgc agaccggaca gcagcgggag
     3121 ttctggcttc cgccgaattc tgcactacag gtacaaaaaa ctttggattt ggtcaggagc
     3181 tccgcatgga tttccagacc aataacagga atatatgatg acatgataaa actgctcctc
     3241 cttacttcaa taccggaaca gcggtttcaa actgctgttc aaaggcatct gcaacctgta
     3301 gaatggttgc ttcatcgaac tgcttgccca caatggacat accgatcggc atgcctgctg
     3361 cattatagcc gcagggggta gaaattgccg gcagagaagc aatatttacg gttaccgtac
     3421 aaatatccgc ctgatacatc tttaccggat cagaaatgtt ttcatgtacg ccataggcaa
     3481 cagacggggt tgtcggtgtc agaattacat cacaggtttc gaaaatgtgt gcatattctg
     3541 cggagattcg ctgcttgagt gccatagcct ttccgtaata tgcatcatag taaccgctgg
     3601 acagagcata atttccgagc aaaatacggc gttttacttc gctgccgaag ccttcgcttc
     3661 tggagttgca gatcagttct tggaatgtgt cgccgacctt gctgcggtga ccatatttga
     3721 tgccgtcgta gcgggacagg ttggaagaag cctctgcgga agcaatcaga taatatgctg
     3781 caacagcgta tttcagactg ggcatgctgc attccaccag ttctgctccc attttttcat
     3841 aagttcttgc agcctgcatc actgcatttc gaacttcatc gtcaataccc tctgcataga
     3901 attccttcgg gattgcgatt ttcagtccct tgatgtcttt tcccagtttt gcggtatagt
     3961 cttccggatt gcgtttggaa gatgtaccat cgtgcgggtc aaatcctgca atggcattca
     4021 aaatccatgc acagtcctgt gcgtttttgg gaatcggtcc gatctgatcc aaagaggaag
     4081 cgaatgccac tagaccataa cgggaaactc tgccgtaggt tggcttcagt ccggtaacac
     4141 cgcagaacgc tgctggctga cgaatggagc cgccggtgtc cgaacccagt gcagctgcag
     4201 ccagagaagc agatacgctt gctgcggaac cgccggaaga accacccggc acacattcag
     4261 tgttaaacgg attgcgggtt tttgcaaaag cagaagtctg tgtggaacca cccattgcaa
     4321 attcgtccat gcttgctttt cccagcatga caatgtcctg tgcagccagt ttttccatta
     4381 cagttgcatt atacggcgga atgaaatctt ccagcatttt tgaagcacaa gtggtcttga
     4441 tgccatctgt gcaaatattg tctttgattg ccatgggaat gccgcaaagc acgcctgcat
     4501 ttcccgcatc aatttttttc tgtgcagcag cggcatctgc cagagcacgc tctgctgtta
     4561 ctgtaatata actctgaatg gtatcatcat attttgcaat gcgatccaga tagctctgtg
     4621 tcagttccgg tgcagaaatt tctttgctgt ccagcatctg acgcagtttt ccaatggttt
     4681 gttgcgtaag atccatatgt tttcctcatt cgtccgcaga cgaatgcctc cttttctgat
     4741 tgttatccgg cgaaaagcac gtacacaacg ggtgtggctt atcgcgatta ttctacaacc
     4801 tttggcacaa cgaaacagtt ttcgtcgttt acagcatttt gcagaatctt ttctgtaggg
     4861 aaactgtctt ccttgatgtc ttcccgcaag tcgttcagat aaacattttt attgtcggca
     4921 tatgcatcat attgaatgtc gatttccttt acggtatcca taatttcaat gatgcttgtc
     4981 atttcagatg ccatttgctc catttcttct tttgtaaaat gaagctttcc caattctgcc
     5041 agataggcaa tcatctgtaa atccaatctt gaccctcatt tctatttctg aaattgcaga
     5101 aatttattcc acggcaaagc cgcagacatc tgctcaaatt tacacaggtt ttattataac
     5161 ataatccctt gaattttgca aggcttttct tacgggaaag cacaaaaaaa cagaatttga
     5221 aaattttttc cggatatgct tgattttttt ccggtttttc agtataatag aatgtgatat
     5281 tgtttggaaa cccttgtgtc tgtcaaaaca tagagtttgg gatttccaag gtagggagga
     5341 ttcataatgc tgactttttc aaaaatgcac ggcattggca atgactacat ctacatcaat
     5401 tgtttcaagg aaaccgtaac ggatccggaa aagctttctg tttttttaag tgatgttcgg
     5461 tttggcgtag gttctgacgg tctggttttg attcttccgt cagaagttgc agatttccga
     5521 atgcggattt ttaatgcaga cggctctgaa gcaatgatgt gcggcaatgc caccagatgc
     5581 atcggcaagt atgtttacga catgggcatg actgacaaaa tagaaatttc gctggaaaca
     5641 aactccggca tcaagtatct gacactgtat cctgatgcca acaacaagat tgaaagcgtc
     5701 aaagtggata tgggaaaggc aatcctcgtt ccgagagaaa ttccggtcaa ttcggatctg
     5761 gaacggttta ttgcacagcc agtaacagtg gacggtaagg aatacgcaat gacctgcgtt
     5821 tctatgggaa atccgcatgc agttgtgttt ttgccggaag tggattctct ggatctggag
     5881 aaaatcggtc cgtcttttga acatcatgag ctgtttccgg atcgagtgaa tacggaattt
     5941 gtccgcgtga tcgatgacca tacgctgcaa atgcgtgtat gggaacgggg aagtggtgaa
     6001 acctttgcct gcggtaccgg cacttgtgcc actgtagtgg cagcagttct caatggattc
     6061 tgcaaaaagg aagaagaaat tctggtacat ctccgcggtg gagatctgcg tattatttat
     6121 cacgaagatg atacagtcac tatgatcgga cctgcaatct atgtttttga aggaaagatg
     6181 gagtatccga atctctaaag caaatgtagg gtgtgttgac acgtgtcttt tagggtgatt
     6241 ttccccgttc gtcgttaaaa atccttgcca tgcgaaagca cgcctgcgaa tttttgctta
     6301 gaacggagaa aaatcacctc ataaaattca catatcatta tattataatg ataatgtcaa
     6361 cgccccctaa atcataattt taatttcata aaggagtatt tcaaatgcca acgatcaatg
     6421 agaatttttt gaagctggga aagaattatt tgttcatcaa tattgcaaaa aaagtcaatg
     6481 cgttcatggc tgaaaatcca gatgcaccgc tgattcgtat gggcatcggc gatgttacac
     6541 tgccgattgc accggtttgc gtagaggcaa tgaaaaaagg tgccgatgaa atgggcgtaa
     6601 aagaaacctt ccgtggctat gaggacagcg gctccggcta tgatttcctg aaagaagcaa
     6661 ttgcaggata ttacaagaag tttggcgttt ctctggaact ggatgaaatt cgtgtcaatg
     6721 acggtgccaa gagtgactgc ggcaatattg tagacatttt tggcgatgac aatattgtac
     6781 tgattacgga tccggcatat ccggtttatg tagattccaa taaaatgaac ggcagaacgg
     6841 taatttatgc agattctgat gaatccaacg gttttgcagc tatgccagat tcgtctgttc
     6901 atgcagattt gatttacctc tgctctccaa acaacccgac cggttccgca tataccagag
     6961 aacagctgaa agaatggatt gcttacgcta aggcaaataa agcaatcatt atctttgatg
     7021 ctgcgtatga agcgttcatc acggatccgg atgtgccgca cagcatttac gaagtagacg
     7081 gtgctaagga atgtgccatt gaaatgtgtt ctctctccaa gacagcaggc tttactggta
     7141 tgcgttgcgg ctatactgtt attccgaccg cactgcatgt aatcgcttct gacggcactg
     7201 atgtcagcat cgcacagatt tggggcagac ggcagggcag taagttcaac ggcgtttcct
     7261 atccggtaca gtgtgcagca gcagctgttt tcacggaaga aggacagaaa cagattcgta
     7321 agaacattgc atactatcag gaaaatgctg caattattgc aaagactatg gaagaactgg
     7381 ggattgaata taccggcggg aagaattctc cgtatatttg gttgaagtgc cctaagggta
     7441 tgaacagctg gggatttttc gattacctgc tccacgaagc agcagttgtc ggcacaccag
     7501 gcgaaggctt cggcaagaac ggcgagggct ggttccgtct gactgcattt ggcgacagag
     7561 aaaaaaccaa ggaagcaatg gaacgcatga agaagctgct ttcttgaaaa aattggctgc
     7621 ctgaaataat ataaaaaagt caaagccgtt ttcggctatg atagaaatga caacggagga
     7681 ttatttatta tgcgtgctgt aatcacagta attggtaagg acaatgtagg tattctgcat
     7741 caggtaagcg gcgtttgtgc aaagtatcag gcgaatgtgc tggaagtgac tcagagcgtt
     7801 ttgcaggata tgttcgccat gatcatgatg gtggacatca cagcaatgac aacagatttc
     7861 acacagctga gtgacgaact gaacagcttg ggtgaaacac tgggcttgtc cattcacgct
     7921 atgcatgaag atattttcaa tgcaatgcac cgtatctaag aaaacgcagg agaagattat
     7981 attatgaata gcactatgtt taatgcagga gatattctgg aaacgatccg aatgattcag
     8041 gatgagtgtc tggacattcg tactaccacg atgggtattt ctctgctgga ctgtattgac
     8101 cccgacatca ataaggcttg tgataaggtc tatgacaaga tcacaagaaa ggcagaaaag
     8161 ctggtggaaa ccggtgaaaa gattgaaaag gaatacggta tcccgatcat caacaagcga
     8221 attgctgtaa caccgattgc tatgctggca gctgcatgcc cgaaggaaaa tccggtaaag
     8281 ttcgccaagg ctttgcagcg tgcagctgaa acctgtggtg tcaattttgt cggcggttat
     8341 tccgcactgg ttcacaaggg attcagtgca ggagatctgg aactgattcg ttccattccg
     8401 gaagcactgg atgtaactac tcgtctgtgt tcttctgtca acgtaggctc caccagaagc
     8461 ggtatcaaca tggacgcagt tcgtatcatg ggtgacattg ttctggaatc tgcaaagctg
     8521 acagcagatc gtcagtgtgt tggaccgtcc aagctggtca ttttctgtaa tgcaccggaa
     8581 gataatccgt ttatggctgg tgcgttccac ggtgtcggtg agccggattg cgttatcaat
     8641 gtcggcgttt ccggaccggg cgttgtacgt tctaccgtat ccaagtatcc ggacgcttcg
     8701 atcgatcaga ttgcagacat catcaaaaag accgcattca agatcaccag aatgggtcag
     8761 ctggtaggtg caaaagcatc gcagatgctg ggcgttccgt tcggcattgt agacctttct
     8821 ctggccccca caccggctgt tggtgacagc gttgcacata ttctggaagc aatgggtctg
     8881 gaaacctgcg gtacacatgg cactacagct gctctggctc tgctgaatga tgcggtaaag
     8941 aagggcggcg ttatggcttc ctccaatgtt ggcggtctgt ctggtgcgtt tattccggtt
     9001 tccgaagatg ctggcatgat cgatgcagca gtagacggta cattgacact gacaaagctg
     9061 gaagcaatga cttctgtttg ctccgttggt ctggatatga ttgcagttcc cggcgatatt
     9121 gaagcgtcta ccatttccgc aattattgca gatgaaatgg caatcggtat ggtcaacaac
     9181 aaaacaaccg cagttcgttt gattccggca attggtctga aggaaggcga tatgttggaa
     9241 ttcggcggtt tgttcggcag tgcaccggtt atgcctgtaa agcggaagtc cgctgcaaag
     9301 ttcattgcac gcggcggtcg cattccggca ccgatgcaca gcatcaagaa ctaatgggag
     9361 aaccatgcag cagtatacaa cagatataat gattatcggc ggcggagcct caggcctcgc
     9421 cgccgctgtt gcagcaaaac gtttttgtcc ggagttatct gttatgattc tggagcgtaa
     9481 ttccagagtt ggaaaaaaga tactggctac cggaaacggt cgctgcaacc ttgggaacat
     9541 ttcaacagat tcccgatatt attatggctc ctgcaaaaag tactggaata caattgtaga
     9601 acagacgcag ccagcagaag cattttttca aagcatggga ctgtactgcc gtcgtgaatc
     9661 tgatggtagg ctgtatcctt gttcgaatca ggcggcgagc gtattagatg cacttcgttt
     9721 gacaatagaa caagagggga tttctgacgt ttgctattgt ttggtttccg atatacagca
     9781 gacggaatcc ggaattctgg caaagaccac acaaggagat ttccttgcaa gtgcggcaat
     9841 tgtcacaaca ggtggatttg ctgctcctaa aaccggctct gacgggtcag cattctcatg
     9901 gcttttgaat ttggggcatc attgcaatac accaaagccg gcattggtgc cttttttaac
     9961 aaaaccagtc ttactgcgac tgttgaaagg tatccgtctt tcggcagaag tgactgcaat
    10021 ttctgaaaat ggcactgttc ttgcggtaga tacaggagaa gtgcagttta cagaacggac
    10081 tatctccgga atatgtgtaa tgaatctctc tgctgcttgt gtgggacagg aaccggctgc
    10141 aatttgtcta aggcttttgc cggaaattcc agaacaacgg ctacttcctt tgttgtggga
    10201 attatatggc gttcggacaa actggaaact ggaggattgg cttaccggct tgctgcctaa
    10261 aaaggcgggc atacagcttt tgcgaagtgc tggtattgct atgtcaatgg atacgcctgt
    10321 ttatgaaatt acgccgcagc aattggagca agttgcgaaa actattcttt cttggaaatt
    10381 tccagtcctc tccagaggca gttggcagga ggctcaagtg acttccggcg gcattccggc
    10441 agaggaaatt gatgcggatt tgcaatctcg tttggtaaat ggcttgtttt ttgcaggtga
    10501 gatcttggac tttcatggaa actgcggcgg ttataatctg aattgggcat ggcaatccgg
    10561 acaattttcc ggagaaaatg cggcgaaaca aattttgcag aaaagaggtg acactgtatg
    10621 attaaattaa cgaatctctc tatgcccttg caattcacgg aagatatttt acggcagaag
    10681 attgcacaac ggctgcatat ttcagcacag gaaattcagt cagtgttata tttgaaacgt
    10741 tctgttgatg cacggaaaaa accgaaaatc actgtgattt tgacagttgc agttgccgtt
    10801 tcaaatgagg aaaaaattct gcggcgtgct gtaaaagatg ctgacattca gtcgtacgta
    10861 ccttattatt atcgtatccc aaaatgtaca gttcccgtaa gccaatcaaa tcgtcctgta
    10921 attgtgggat ttggtccggc gggcatgttt gcggcattaa ttctggcaaa ggcgggattg
    10981 cgtccaattg tgctggaacg cggaaaatgc gtggagcagc gacaggaaga tgtgacaaat
    11041 ttccgaaaaa ccggcatgct ctgtacagac agcaatgtac agtttggcga aggcggtgca
    11101 ggaacatttt cagacggcaa actgacgact ggcattaagg atccgcgaat tcgttttgtg
    11161 ctgcaaagct ttgtagaatg cggcgcaccg gaagaaattc tctatctggc aaaaccacat
    11221 atcggaacgg ataagctggt acatgtcgtg cgagaaatgc ggaaaacaat tcaacgatac
    11281 ggcggagaga tcctctttca ggctcacatg gaaaagctgg tacagaaaaa tggcaagctg
    11341 acagctgtac agtatcaaaa ggacggaaaa acgacagaaa tccttacctc tcactgtatt
    11401 cttgctgtgg ggcatagtgc tcgagatgtg tttgaaatgc tgtacaatgc gaacatttct
    11461 atgacgcaaa aaaattttgc agtcggtatg cgaatcgagc attcgcagga ttggttaaat
    11521 catgccatgt acggcaggga agcctctccg ccggagcttc cggttgcaga ttataagctg
    11581 gctgtgcact tgccgaacaa acatagtttg tacacatttt gtatgtgtcc gggtggggaa
    11641 gtcgttgcag catcttcaga acaacatcga ctggttgtca atggtatgag taattatgcc
    11701 agaaatggca gaaatgccaa tagtgcatta ctggtcggcg tttccgcggc agagttgcag
    11761 gattcacacc cgttagccgg aatgcggttt caacagaaac tggaagaagc tgcatttcaa
    11821 gctggcggcg gcaattatta tgcaccagtt tgctgtgttg gcgatttttt gcaaaaacac
    11881 gctgcgactg gttgcggaaa aattgaacca acgtatcagc cgggtgtcaa ttggatttca
    11941 ccagatgaat atttaccggc atttgttgca gaaactttgc gacttggaat tccagccatg
    12001 aatcgtaaga ttcatggatt tgcagaaaca gatgcagtat tgaccggcgt agaaagccgc
    12061 agttcgtcgc ctgtacgcat
//
LOCUS       DAWBGR010000066        11949 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1608_length_11949_cov_5.672020, whole genome shotgun sequence.
ACCESSION   DAWBGR010000066 DAWBGR010000000
VERSION     DAWBGR010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11949)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11949)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11949
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1608_length_11949_cov_5.672020"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2028..2037
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7196..7205
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2821 a         3049 c         3281 g         2778 t
ORIGIN      
        1 agcagctgat ttgtaatcag caggtcgggg gttcaagtcc gtccaccagc tccaatattc
       61 tacaacatac atatgttaca tgggggagtt cccgagtggc caaagggggc agactgtaaa
      121 tctgttgctt ctagcttcga tggttcgaat ccgtcctccc ccaccagaaa acaggcaaac
      181 ccttcgggag tttgcctgtt tttctttttc gcatgtgtat ggattgtaga gcaatacata
      241 tcaggcagcc ctgcataagc tttgcacggt gctgtctgtg cagaatcggc ggacaggctt
      301 tgatgccggt tcgcgaaacc cataccatag gaatgaggag agaattatgg ttcgtgagga
      361 tttgagaaat gttgccatta tcgcacacgt tgaccacggt aagacaacgc tggtagacga
      421 aatgctgaaa cagggcggtg tgttccgtga aaatcaggct gtggcagaac gtgtcatgga
      481 ctccaacgat ctggaacggg aacgcggtat cacgattctg gcaaagaata cttcggtacg
      541 ctacggcgat accaagatta acatcattga tacaccggga cacgctgact tcggcggcga
      601 ggtagagcgt gtactgagca tggtagacgg tgccattctg ctggtcgatg ccgcagaggg
      661 tccgatgccc cagacacgtt tcgtactgtc caaggctctg gaaatgaacc tgaagatcat
      721 tgtggtcgtc aataagatcg accgccccga tgcccgtctg gacgagatcg cagacgaagt
      781 gctggaactg ctgatggatc tggatgccag cgaggaacag ctggagaata cgccgttcct
      841 gtactgctcc ggccgtgcag gtaccgcaag ctttgacccc cacggtgagg gcaaggatct
      901 gaagccgctg ttcgacacca ttatgtccta tgtgaatccg ccggagggcg atccggacgc
      961 accgttacag atgctgatct cctccattga ttataatgaa tacgtgggca gaatcggtat
     1021 cggcagaatt gaaagaggta ccattcaggt gggacagcag gtactgctgg gcaactacca
     1081 cgatccggaa aatacccgcc ggaacaagat tgtttccctg ctgcaaattc agggcttgca
     1141 gcgtgtgccg gcagagagtg cgaccgtagg cgacattgtc tggatcagcg gtatcgaaaa
     1201 catctcgatc ggagatacgg tctgtgcacc ggacgacttc gaagcactgc cgttcaccaa
     1261 gatcagcgaa ccgactgtgg aaatgacttt cagcgtcaac gacagcccct ttgccggtca
     1321 ggaaggcaag ttcgtcacct cccgccagct gcgtgaccgc ctgatgcggg aactgctccg
     1381 tgacgtttct ctgcgtgtca ccgaaaccga cagcactgat gcgttccgcg ttgccggcag
     1441 aggcgaaatg cacctgtcca ttttgatcga aaacatgcgt cgtgagggct atgaactggg
     1501 cgtttccaca ccgcgtgttc tgttccacga agtagacggc aagcgttacg agcccatcga
     1561 ggaactgtcc attgacgtgc cggaagactg tgtcggctcc gtcatggaaa agctgggctc
     1621 ccgtaaggcg gaattgcagg atatgcaccc gcagggcaac cgtacccgtc tgacattttt
     1681 gatcccgtcc cgcggtctgt tcggttacaa gagcgaattc ctgaccgaca ccaagggcga
     1741 gggcatcatg agcagtgtat tctatgaata cgcaccgtat aagggggaga ttccccgccg
     1801 cagcaccggc tctctgatct ctttcgaaac cggcgaagct gtcacctatg gtctgttcaa
     1861 cgcacaggaa cgcggctccc tgtttatcgg tgccggcgta ccggtgtatg agggcatggt
     1921 cgtcggtgca tcgccgaagg cagaagatct ggtagtcaat gtctgcaagc ggaaacacct
     1981 gaccaatacc cgtgccagcg gcagcgatga tgctctgcgt ctggtacnnn nnnnnnngtc
     2041 tggtaccgcc ccgcaatctg agtctggaag actgtctgga attcctggca gatgatgagc
     2101 tgctggaagt aacgccgaaa tcgctgcgga tccgcaagca gatcctgagc aaccagcagc
     2161 gtgccaagat gcgtggtaag aaataagtcc tagcattttt gggttccccc gtttcagaga
     2221 ggaatgaaaa tatgaaacag ttttggaaaa agacactttg ggcagccctg ctgacagcag
     2281 catttgcact gtctgccacc ggctgcggcg atgacaaaca gccagccagc agcacggcga
     2341 attccgccag ctctgcggca caggtagcag acagcagcac tgcaccggaa gaaactgaca
     2401 gcagtgcgga aacaaacgtg ttccagtatg agatgaacga tgacggcacc gtcagcatca
     2461 ccggctatca aggcatggaa gccgttttgg aagtgccgtc taccgtggac aattatgtgg
     2521 tttctgccat ttccaaccat gcctttgagg caaaccagaa tctcacggaa gtgactttgc
     2581 cggagggctt gtcggaaatc ggcgaaagtg cgtttatgga ctgcggcagt ctgaccaccg
     2641 tacagattcc ggaaaccgtg gcacagatcg accgtgcagc ttttgcaggc tgttcggcac
     2701 tgaccagcat ttctctgccg gaaactgttt ccaccgtcat ggaggaagca ttcaccggct
     2761 gtaccagcct gactgatctg actgtggcga atgcgtcact ggaatatgac cgctggggac
     2821 tggtagaggg cagcgagccg ctgggcgtga cgattacttg cccgtccggc tctgccatag
     2881 aaacatgggc agctgccaac ggcatcagca caaaaacatt cggataaatc ataaagacaa
     2941 agcgtgggac aacgcgggtg tagttcccta caggacaaac catatggtgt gtaggaaaaa
     3001 ccggcagctg cccacgcttt tttgttttgg aggaaaacat gatgaaacac atctggaaac
     3061 aggcactggc tctgctgctg gcatgcggca tggcggcagg tgcagccggc tgcggcgaaa
     3121 agaaagataa ggcggaaagc tctgcggcag agagcagcac agcggattct gccgattcca
     3181 tagagccggt gacagaatcc gatccggaca acatgaacta caagctgacc tacgataagg
     3241 acaaggtgcc ggacgaactg gcaaatacca tcgccctgta cttttatgca gtggacaccc
     3301 agaactatga cctgtatctg gagcagatca acccgctgta ccggacatcg ttggagagcc
     3361 tgctgcaaga gcagtacggc tacggactgg agaacagcat ggaacagctg caccagaatc
     3421 tggtgaacta tgccggcacc gataacttta ccattcagag tctggaactg gcacaggcac
     3481 aggaagtgct ggcggaggac ttcgaggaag ataccaattt tgtgcaggag tacctgaacg
     3541 cctatacaca ggcattcggc gaggaattca ccaaggatct ggaagaacag tccgatgcga
     3601 tctatgatat tgccgtgacc atgaagggcg agaacagcga cggcgaggag atcaccattc
     3661 tggacagtct ggaaattctg gcggcagagg cagacggcag cttcggcgtg ctgggataag
     3721 cggaaaccga cacaaacggc agtacggctg cccgcgtaaa acgtgcatct gttttggctg
     3781 aaaagacatt tccaaaatgg attttcatga tttccctgtg aaatgcttga caatttcgcc
     3841 gaaaaagcgt ataatagaaa gtaccgtgtt gcaattttca atgaaaatgc ggaaaagttt
     3901 tggaaaaaat gaaagcagta ggataagctg tatgcttttt tccacaggag ggtattgaca
     3961 atgcatagag tatacaattt cagtgcaggc cctgcggtat tgccggagga ggttctgcgt
     4021 gaagcagccg atgaaatgtt ggattatcag ggtactggca tgtccgtaat ggaaatgagc
     4081 caccgcagtg ccgcttttga tgagatcatc aagaccgcag aacaggatct gcgtgacctg
     4141 atgggaatcc ccgataatta taaggtactg ttcctccagg gcggtgcctc tcagcagttt
     4201 gcagcagttc ccatgaacct catgaagaac ggcgtagcag actatatcgt taccggtcag
     4261 tgggcaaaga aagcttatca ggaagcccag aagtacggta aggctgtcaa ggtggcttct
     4321 tcggaagata aaaccttctc ctacattccg gactgctccg atctgcccat cgatgatgat
     4381 gcagattatg tgtatatctg tgagaataac accatttatg gtacaaaata taagaagctg
     4441 cccaacacca agggcaagac gctggtcgct gacgtgtcct cctgcttcct gagtgagccg
     4501 gtcgatgtga ccaagtacgg cgtgatctac ggcggcgtac agaagaacgt gggacctgcc
     4561 ggtgtcgtta tcgccattat ccgcgaagac ctgattccgg aaaagccggc tgtggaaaac
     4621 acccccacca tgctggcatg gaagacacag gcagacgcag attctctgta caacactccg
     4681 ccctgctatg gcatctacat ctgcggcaag gtcttcaagt ggctcaagaa aatgggcggt
     4741 ctggaagaaa tgcagaagcg gaatatcgcc aaggcaaagg tgctgtatga tttcctggac
     4801 gagagcaagc tgttcaaggg tactgtccgc aaggaagacc gttctctgat gaatgtaccg
     4861 ttcgttaccg gcgacaagga catggatgcg aagttcatca aggaagcaac tgctgccggc
     4921 ttcgtgaacc tgaagggtca ccgcaccgtt ggcggcatgc gtgcatcgat ctacaacgca
     4981 atgccgatcg agggcgtaga aaagctggtt gcattcatga agcagtttga agcagacaac
     5041 gcttgaggag aggacagcat aatgaaatat tccattcaga cactcaataa gatttccaag
     5101 gcaggcacag accggttctg tgatgcttat accatttcgg acgatgccaa ggatccggac
     5161 gcaattctgg ttcgcagtgc atcgctgcac gatacctcgt tcggggataa cctgaaagcc
     5221 attgcccgtg ccggtgccgg cgtgaacaac attccgctgg atcgctgctc ggaggctggt
     5281 atctgcgtat tcaatacacc gggtgcaaac gccaatgccg taaaggaact ggtgctcacc
     5341 ggtctgctca tttcctcccg taagattccg gaggcaatgg catgggcagc aaccctgaaa
     5401 gacggcgaaa cctctgtggc aaagcaggtg gaaaagggca agagccagtt cgccggtccg
     5461 gagatcaagg gcaagacact gggcattatc ggtctgggtg ccattggtgc actggtggca
     5521 aatgccgctg tggcactggg catgaaggtc gttggtacag acccgttcct gtccgttggt
     5581 gctgcactgc gtctggatcc gtcggtaacg gtcgtaaaga cacaggacga cgtattcgct
     5641 gccgcagatt atctgaccat tcatgtaccg ttcaacaagg acacagaggg catgatcaac
     5701 gctgccgcga tcgcaaagat gaaggacggc gttcgcgtgc tgaactatgc ccgcggcgaa
     5761 ctggtcaacg atgaggatat gatcgctgca ttggaaagcg gcaaggtggc ttactactgc
     5821 accgacttcc ccaatgccaa gacctgcaac gccaagcaca tcattgctac cccgcatctg
     5881 ggtgcatcga caccggagag cgaggacaac tgtgcaagca tggctgccga tgagctgatg
     5941 gcttatctgg aacagggcga tattcgcaac agtgtcaacc tgccgaatgc acagcttcag
     6001 gcagtgtaca ccaaggtttg cgtcattcac cggaatgtgc cggcaatgat ttctgccctg
     6061 acttctgtca ttggcgaagc cggtgccaac attgagaaca tgaccaatgc cagcaagaag
     6121 gattacgcgt ataccattct ggatattgac ggcgtaaccg acggcatgat cgaagcactg
     6181 actgctatcg acggcgtact ccgtgtacgt gtgatcgaaa agtgatctgt gcgtatagcc
     6241 tttggcatct gcccgaattt tcgggttgaa acggtgtgaa agcaccgaat agattgcatt
     6301 tccagagtag gaaatcgtgc gttaagtgtc cgtcagacgg ggagttgctg tcgggacgaa
     6361 aagcgagaac gatcgcttgc ggtacggttt ccgcatcccg ctgaatgtat cataagaagt
     6421 cagaaatctc tcagagaaaa cacctcttat gctgcacgaa gcagaaacgt tttgtactgc
     6481 cgcaaggcaa aggaggtatt ttttcatgag agattttttg tctgtattta cggattccgc
     6541 aaaggaattc accaaggttc gcagtctggt gactacatcg ctgctggtgg ctgtttccat
     6601 ggtcatcgag atgttttcca ttgacatcgg ttttgccaag ctgaactttg cgttcctcgc
     6661 tatcgccatt atcggcatgc tgttcggtcc gtgcatggga atgatggcag gttttgcctg
     6721 cgacatcgtg ggctatctgg cacaccccag cggcggcttc ctgccggcat atgtgctggt
     6781 cggcggctta caggggctgt tctacgggct gttcctgtat cagaagtgga gtggtcacag
     6841 cattcagttt gtcagcaaca agaccggcaa gactacggac atcaccctgt atctccgtgc
     6901 gatcgtggca cggctggcgg acgttgtcat catcaacctg ctgatccaga ccaagctgaa
     6961 tttgcactac ggttttattc cgcaggaggc gtatggtgcg gcgatcgtgg cacgtgtggc
     7021 aaagaatgtc atcgagttgg cgatagacct gccgctgctg ttcattctgc tgccggtggc
     7081 actgaaagcc taccagcaca ttttccggcg gtcggtatcg ccgaaagtgg ctggataatt
     7141 tcaggcaacc cacaacttcc gcatataagg caataccgca caaagagcgt ctggcnnnnn
     7201 nnnnntattc actccgcttt ttgtttcatc tgacgaaaaa tttgacgacg catctgtcgt
     7261 acaatctttg tgcggtattg ccgtaaatga aaaccacccg cacacggtac tttttactct
     7321 ggaaaagtac tatgtcgggt ggtttttgtt tcggatattt ccctaatctc ccagtcgttt
     7381 tatcatctgg agatacccca gcgattcgta acccagccgg cggtacagtg cctgtgcccg
     7441 ttcattttcc ggttcggttt ccagccggag cattaccgca tcgccccgca gctgttcctc
     7501 cagccattgc agaaaacggc tgcccagtcc ctgcccgcgg tatgcgggac ggatatacag
     7561 atcttccacc cagaccatga caccgccggc ttcgtgctgc atcatgcggt tggtgacgga
     7621 aaagcctgcc accgcatcat cacactgaaa aatatagccg gacagatact ggtcggaacg
     7681 catcatctcc gcaaacgtcc gccggtgata ctctgcggga atggggtgca gcaccgcatc
     7741 ggagtgaaag aattcctctg ccatggtgta gaagtccgtt tcgtctgccg ctgtgatcgg
     7801 acggaatgtc agttcgctca tcggtgtccg cctccgccgc cgtggctgcc gccggaagaa
     7861 ctgctgtggc tgctgccgcc gcctccgcca ccgccgccgc tgctctcgga ggagatcttc
     7921 gtgcggctgg tgtaggttcg caggtactgg tcggatcgct ggaggtactg cacatcgttg
     7981 gcaaccagat agttttgcag gctgcccgca gccttgaact tataccgatt ccggatcgac
     8041 agcacggaaa tgccaaaagc gatcacgccg atcagaacgg caaggatcac aatggttccc
     8101 cagctgagta ttgcggtgta ttcctctggc agtttgctga cttcctgcac ctgaccgtcc
     8161 tgcatgatga gatactttcc ggtgtctttg ttgtaggtgt agtatttgtc agtgggaacg
     8221 ccctggtctg catagtattt cagatcggaa cagaagatgt gtatcgcaga cggcaggtct
     8281 tcttcgccgc ggggcagata ttcttccaca tcctggatca tggttcccac acgattgtag
     8341 tcctcattgt ccgtataata gaactgtccc ttgccgctgg tggagaggta gtcgaacaga
     8401 gaggtttctc cggacatatc cagatagagg aatacgccgt cggtattcat gccgtagagt
     8461 tcctcatagg tttcatcgca gaatgcaatg gtgtctgcat ctgaatcgtc cccgacggca
     8521 gtgctgccga tccagagagc gacgttcatg ccgctttcgt ctgcggcttc ctgtaattcg
     8581 tccattgcct cctgaaattc tgcatcggac aggaagtcgc cctcgtcaca gaggtagaca
     8641 ttgctgctgg aatcctcggt ttttgccgaa acagcgggaa ttgcgagatt gcagatgcag
     8701 ataccggctg ccagagctgc cgcggtctgc ttccagatcg ttttcatcgg aacatcagcc
     8761 ctcctatcag tactgccagc cctgtaattg ctgccgccag cacgccgctg aaaagcagtg
     8821 ctttccccca gctgagaggc ggcgtgcctg cctgcgtgcc ggtctgcccg ttcatggcga
     8881 agtcgtagtt ctttccatgg tactggtagt gcatgaacca taccggcagc agagcataat
     8941 cgtgctggat attttccatt tgtacattgt gagaagtcac cacgtgagaa ctgtagccgc
     9001 tgatggattt ttccacggtc tttacggcgt gttcgtcgat ccgcttatgg attcggggca
     9061 ggatctggtc gtaggggaca tcgtatttct gtgccataaa gccgctgagg tacttcatgg
     9121 agaatgcttt catcttgtca taggagaacg gctcaatagc gttcatgaga gcatcttcga
     9181 ttttttgaga gccgtctgcg ggaacgccgc gataggggat ctttgccaga cgttttacag
     9241 cgaattcttt ggtttcggtg acccgatagt ccccatcccg ccagctgcgt tcctttttgc
     9301 ccagtgcatc tacatgggtc ttggcatcac agttggtgag ccagaacggt acataaacac
     9361 cggacatttt ttccagctgc ttttccgagg tgaagcttct ggggagaaac caacgctttt
     9421 tgcaccagct gtggaatttt tccacagctg cttcccgtgt gaccgcaaag ggcagcacat
     9481 aggccggctt gtattctccc gtcagccgtc cggcgagtac caccggagtg tggcagtagt
     9541 agcagaatgt ggcggaagtt tccgcttctg caatgatctg tgccccgcag ttctgacagg
     9601 tgtagagccg tgtttcgttc tcgaacgcct tgtcatcagt ttcgctttcc agcgtttccg
     9661 ccgaagcgtc tgcttgctga tctgcttgtt gttccatgtt ctgatacagc tgctgcattt
     9721 ctgcttcggt gtaggtgctg tcgcagtatt cgcagctaaa attctgatgc tccggatcaa
     9781 aggtcagttc cgcaccgcag ttaggacatt tgtattgtac ggtattcatg ttgttctcct
     9841 ttttgccgtt tatccacgtt tgttgccgca gttggtgcag aaattgccgg tattgacggt
     9901 gccgcaggaa caggtccatt caccggcggg agccggcttt ttgctgccgc agttggtgca
     9961 gaaattgccg gtgttttctg taccgcagct gcacttccaa gagccggcag gagccggttt
    10021 tttgctgccg cagttggtac agaagttgct gctgttgaca gtgccacagg aacaggtcca
    10081 gccgtttgca gctgctgcag cgggggctgc ctgctgttga gccattgcct gctgctgcat
    10141 ctgctgggca ttggcctggg aagcacttgc catgaagttg ccggcaccct gcatacccat
    10201 gcccattgcc atgtacgcct gtccggcacc ggcagtatta gagccggcgg cctgaagtcc
    10261 ctttgccaca gccgactgta caaagccttc ccgtacagag gggtcgccca tcatggcacc
    10321 ctgattccgc atgttgatga gagccttgct ttcctcgtca tagctgatgc tgccgatacc
    10381 cacagcacaa atatccacgc cccgtgcctg tgtccagtcc tcgtccagaa cggttgccat
    10441 atacttggac agctccatgc tcttggacgg cagggcggag atgcggacac cgtctacaga
    10501 catctgtccg atcgccgact ggagagccga caggaattcc atgttgaact ggtcgcccaa
    10561 ttcgtcaaat gtcaggctgg tagcgttgcg gggaaccact tccatgaaga atttcagcgg
    10621 gtctgtgatc tttatcgaat agctgccgtg acagcggacg aacaattctg cattgtagaa
    10681 attgtcaaag tattgcaggg cgttgcgtgt gccgaacttg atgttgcgga tctcctgtaa
    10741 gttgatgaaa tagacctgct gggacttgcc cggctgtccg ccgaacttga agcgttcgaa
    10801 agactcccgc acagaatcct tcagctgtcc gttgaacatg gacggagagg aggagaggaa
    10861 tacctcataa cagccttcct cagcagtgta gtccatgatc cgaccgctgt ccaccaggat
    10921 catcatctgg ttcggtccaa cctcaacgcg ggaaccattg gaaatgatgt ctggtgtgcc
    10981 cttggtgttg ctgccgtgtt tgccacgctt gatctgtaca ccctttgtca tgacggtgtt
    11041 ggcacccatg tcaccggctg tgatgtactc cagccacgaa tctgccagtg tgccgccaac
    11101 ggcacctgct gctgcacgaa tcaaacccat attgataacc tccagttctt tcataaggcg
    11161 aagcaatgca aaccaacggt ctggtttatg atatgcgagt gatatgcgga caatgccgct
    11221 ggtttacatg ctgatttgca ataatattat cataattata ccataaaaaa atcgacttgg
    11281 caagtgaaaa ttgacatttt ccatgaaaat ctccgaaaaa aattgtaatt tttttcggag
    11341 atgaaaaaac acttgacttt ttccgagatt tatggtatac taatgtttgt ctgaggcaac
    11401 actttcgaaa gaatggggta tagccaaggg gtaaggcaac ggactttgac tccgtcattc
    11461 cgctggttcg aatccagcta ctccaaccaa aagtgaaaag agaaaacccc gtccagctgc
    11521 tgtatttgga cggggttttc tggacattga gaaaggatcg gacatctatg ggcgaacata
    11581 aactggcaat cgttctcggc atcgtcaccg tggtgtatat cctcgccatg atctttctgt
    11641 tctggcggta tcggaaagcc atggggaacc tctcggaaaa gctgcgggaa tgccgtgaaa
    11701 aagaggaacg gcagcaggaa caggagcagg tgaaagctga ggaggacatt gttacgatct
    11761 atcagaagcc ggaagccgct gctcctgaaa ccgaaaaaac gcagccggct cccgaagaac
    11821 cgactgcgta agttaaggaa acgctgaata aagcccgcct gacggctttg cacgcaatct
    11881 gcttgcagat tttccgtcat attgcccaga aatcccttgc catacatcaa gtatgcctgc
    11941 ggaatttct
//
LOCUS       DAWBGR010000067        11945 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1610_length_11945_cov_5.072750, whole genome shotgun sequence.
ACCESSION   DAWBGR010000067 DAWBGR010000000
VERSION     DAWBGR010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11945)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11945)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11945
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1610_length_11945_cov_5.072750"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7637..7646
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3183 a         3235 c         2709 g         2808 t
ORIGIN      
        1 atttgcaagc agatggcgta caaagtcgcc agacgctctt tgtgcggtat tgcctaaagg
       61 aaacaggtca tcggcatatt ccggtgacct gttttttcag acggcttgcc gcacgagaaa
      121 attcctgctg gtgaaaattt gtgagaatcg gatttccctt gacatttccc gcaggcaatg
      181 ctataatcaa ggcaataccg cagataaagg agaatttact atgccaaatc aaccggaacg
      241 tttccgtgac accagcctgc ctattattga acgggtgaaa gacctgcttt cccgtctgac
      301 catagaagaa aagatccacc tgctctccac ccatcagctg cctgtggaac ggctgggcat
      361 cggagaatgg tatgtgggtc aggaagtggc acggggctat gtttcccgcg aaaagacaga
      421 gccgtctacc gtgttcccgc agccgatcgg gctggcttct acctttgacc cgaacctcat
      481 ggaacagctg ggagaaattg ccggcgaaga agcccgctat tatcaccgga aagaccccaa
      541 ggggcacctg atgctctggg gacctacagt cgatccggaa cgtgatcccc gctggggacg
      601 cacagaggag ggctacggcg aagacccgtt tctgatcgga gaaatgacca ccgcctatac
      661 gcagggcatg gccggtgacc acccgacata ccgccgtgtg atccccacac tgaaacactt
      721 ttgtgccaac aacaacgaaa aagaacggaa cagctgttct tccaacgtca ccccgcgaac
      781 actgcgggaa tactattacc gtgcctttga agcttctatc gtccgcggcg gcacaggctc
      841 tatgatgacg gcttataatg agctaaacgg cgttcccgcc tgcatgaatc ccgacctgaa
      901 aacgctggta aaaaagcagt gggggctgga attcattgtg acagacggag cagatttttc
      961 ccagaatgtg ctggcacacc attctcacgc cacccatgca gaggctcttg ccaactgcct
     1021 gaaaaacggc aatgatgtga tgaccgatga agcagacatg gtggcggcag cagccagaga
     1081 tgcactggac agagggctgc tgacagaagc cgatattgac cgtgctgtgg ggaattctct
     1141 ctccggacgg ttccggctgg gagaatttga cggggacagc tgtccctata acaccgagcc
     1201 ggcggaaacg gacacgctgc tgcaccgtgc agtgaaccgc tgtgctgcca tggaacagat
     1261 gtgtctgctc cataaccgtg ggatcctgcc gctgcaattg cagcctgatg ccagggtcgc
     1321 tgtgatcggg ccactgggaa acgagaacta tcgggactgg tacaccggcg tttcttccta
     1381 tgccgtaccg attttggagg gactgcggca gcagctgggg gcagaaaatg tgctgtttga
     1441 tgacggttat tctgtggttg cccttcagtc tgtggaaacc ggaaagtact gcaccgtttc
     1501 tgcggacggt acgctccgtg cggcggcaga acagatcggc gaacgggaaa cctttttgct
     1561 tcacgactgg gattttggca gcatgaatct ccgtgcctgc tgcaacggca agtacgtcac
     1621 cgaaaacggc acctatcagg cagtcagcga tacgccctat gagtggttca ttcgggaatg
     1681 gctcaaaccg gagccgtatg gagatgccat gcggtttggc agctggcatg attgtcctat
     1741 ggacatcgca ctgcagccgg acggcacgct ccgtgcagtt tctcacggaa agcccacacc
     1801 agaccggctg ttccggctgg tgccggtgga ggacggcatc acccgtgcgg cggctctggc
     1861 gaaacaggcg gacgtggtca ttctctgtct gggcaaccac cccatgcagg tggcacggga
     1921 gtgctatgac cgtcccgatc tggaactgcc ggcacatcag aaaaacttgc tgcgtgctgt
     1981 acaggctgcc aatcccaaca ccgtgctgac tgtggtttcc agctatccct atgctctggg
     2041 ggaagcgatg gaactgctgc cggcgatcct ctataccacc catgccggac cggaactggg
     2101 caacgccgta gcggatacgc tgctgggaaa aaacaatccg gcggcacgct gtccgatcac
     2161 atggtatcgt tccgcacaag atctgccgga catcatggaa tatgacatca tttcggcaga
     2221 gcgtacctat ttatatgaca cggttgaacc gctgttcccc tttgggcatg gtttgtccta
     2281 ttccagtttc gtctaccgtg attttactgc ggaggagaag ccggaaggca tgctgttccg
     2341 gctgactgta gaaaatacct ctcagcggga cggggatgaa gtggtacaac tttactatcg
     2401 ggcactgcac cctcgtgtca agcgtccgct ccgacagctg tgcggcttcc agcgagtacc
     2461 agtgccggca aatacagcgg tgccggtgac aatttttgtt ccgtggtatg ctttggaata
     2521 ctatgacgtg acacaggaaa aaatgctggt ggaacagggc gagtaccgct tttctgcggg
     2581 ggcatcttcc gcggatattc ggcaagaatt ggaatgcaca gtttccggtg aagtgatccc
     2641 accgcgaaac ctctcccatt ctatctgcat caagaactat gacagcaagc acggcataga
     2701 aatcactctg cgattctcgt accgaaagaa cgactggtac ggctgcacca atgactgggg
     2761 cggcgggttt acctttgccg atgcagattt tgccggctat accaaggcag aactgtgggc
     2821 agcagcacct tgttcgcagg caggtgttgc catctatgca ggagaacagc agctgggcag
     2881 tgtggagatc ctgccttctc gcagcatgga cgatttccag ctgtacacga tcccgctgga
     2941 gccctatacc ggacaggcag acctgcggct ggaaatcacc ggcatggcaa gtgtgtaccg
     3001 gattcagctg ggttgaggca atacctggac ttgtttgaaa aagggaattt tctcctaacg
     3061 gaaacactgc ataaagccac acatgacgct ccgcctttcc cacagttctc atacctcccc
     3121 aaaaacaaaa aaagacagac cacggtctgt cttttttttc tttcttcaaa gcatggatat
     3181 tccattgcgt gcaatctttt ccgcctgctt ttttagacga agttcccgaa aaaaatcccg
     3241 caggatctgg gcacattccg cctgcaataa accggcatgt acctccggtg tccagttgta
     3301 gggcagagtg aacagctgtt ccaccgaatc cactgccccg cccttggggt cggctgtgcc
     3361 atagaccacc tgttccagac gggcatgcag aatcgctccc gcacacatgg ggcaaggttc
     3421 caacgtcacg tacagagcac tgtccagcag ccgccagccg cccagtgtcc ggctcgccgc
     3481 atcgatcgcc atgatttctg catgagcaag aggcgaacgg tctgcttccc gccggttata
     3541 tccctcgccg atgatccgtc cggtggattt ctgcaccacc actgccccca caggaacttc
     3601 gcccattgcc gcagcctgtg ctgccagttc cagagcacgc atcatgtacc gtgccttggg
     3661 gacaaatccg gtttccgtca aaaaatcacc tcgatcacca tcagaatcac acagaggatc
     3721 agcacaatgc tcagcggtcc cacatgcatc accgtataga gccgttcccg cagggtcagg
     3781 taatgcttct gtgtcagcag ggcgggattt ttacgggtgg gacgaatgga cagcaggcag
     3841 agcagaattc ccgctatcag cacgagaaac agaaacagca agcaatacga ctgccaaaac
     3901 gacgactgcc agatctccat tgcaaacaac ccgaacagca tcacatggta cagcagccgg
     3961 cagaatatag ccggtaccac agaaccactc cgcagaacgg cgacaccgga aaccagcgaa
     4021 aaagtcagtt ccatcaatat cgtggtgcag ctatgggaca gacaaactgc aacaactgct
     4081 gtcaggatca ccgcatccca gtccccgaac tgccgcagca catgaagcat atccccatgc
     4141 agaagcagtt ctttgcagat gctgatcgcc agcacttcaa aaaccagata aacgatctgg
     4201 taagaacgcc gcatatacga aacggtgtaa tacgcttccc ccagcgtact gctgctcaga
     4261 aaattcaccg gcgaaaacag cagccacaca ttagcgaccg caaaaacgat cagtgtcacc
     4321 gcaatagcag ccggaaactc ccgcggtgaa tccagcttcc agtgatacgc cacacgccgc
     4381 ggcatgcgga aacagcaccg gaggatcagc acaggcacca tgaatttcag caccgtcttg
     4441 cacatcagaa tgaccagaac cgccgtctgg ttgccgtaaa cagtgccgtc actgtagcaa
     4501 tagcctacgt ccattccggc tatctgagcg atgcccatca ggatcgctgt cagcaggttt
     4561 tcgatcagca gataggcaaa tatcgccatg ccgatcacca tggtgatctg tatgagcgac
     4621 cgccgttctt ccgcagccgg gtctggttca tgaaaccctt ttccctcgat gtaaaccgct
     4681 tcttttttct gtttcttgca gcgaaaggca tatgggttat cttctttgtt ccgccatgca
     4741 cggaatgcat gattatatgc ttccgaaaca ttgctgtagt tggaatctgc aatcacgtcc
     4801 accgtatccg aaacggcagc agaaggcttc tccagatttt gtaatttcat atgttattac
     4861 gctcccctca agcgtcatct caaacggaaa cgcccttctc cgcggcattt cctttcaggg
     4921 aacactgcat aaagcccgcc tgacggcttt gcacgcaatc tacgtacaga ttttccgtca
     4981 tattgcccag aaatcccttg ccagacaact gcgttcgagg caggattctg ctcgagaaca
     5041 aaggcgtgtc gcactaagat tgattgcgac catacagtct gcctgcggaa tttctgtgta
     5101 atctgccgaa aaatctgaat acacatctta cgcacaagct ttatgcagtg ttccctttca
     5161 ttcttcacaa tttttgccgt gtgccctgtg cacaacggaa caacattttt ggttgtatat
     5221 caaattgcta tgatttttcc cattttttct actcttatta taccatgcca ccgaaaatga
     5281 tggtttagtg aagtggaaat aatatcgtta tgttttttga aaccttcata tcagctttgt
     5341 gtataagatg aataaatggc aacaggacat aaaaagcaac aggaatcgtg gggttcgcgg
     5401 ggaatgtttg agaggagatg ccttgaaaaa agcctcctcc cttgcgaata tctgtcattt
     5461 cgcaagggag gaggcttatc gataagagga aatcagtttt ggtaaggcaa tttgtcaacg
     5521 atatgcacca aaaattgcat cagcacaacg gcatcatcgc tgccaacttc gctgtcgccg
     5581 ttgcagtcgg catttttctt agtggcatcg tccagcataa ccgcaccagc acaggctttg
     5641 ttcagcagga ctgcatcggt gatgtccact cgtccgtcga ggttgaggtc gccgtagagg
     5701 gcggtgggtt cggtttgggc ggaagatgtg gtggtttggt tggtagttgc agtggtctgt
     5761 tttgctgtgg tagtttcggt ttgttttgca gtagtggcag tggtttgctc tgccgtggtg
     5821 gttgtgcttt gttttgtcgt agttgcggta gtttgctttg ttacggtagt ttcagtctgc
     5881 tttgcagtgg ttgtggtttg ttctggtgtg gtagtcgttg tttgttcggt ggtagtcgtg
     5941 gtagtttgtt ttgcagtagt ggtagtttct gtagttgtgt ctgtggttgt cgtggtaaca
     6001 atactgttca aggacacaaa gggaatctct ttttctgccg cataagtttc cgcatatgaa
     6061 ccggtatagc cgtaaatggt cagatcgcta cagcttttaa aaacgttata atcgattttt
     6121 gtcacactgt ctggtatgat gatttcagtc aggctaacgc aattgtcaaa agtcatagca
     6181 tcaatgctcg tcatgccatt tggaatcgtg atcttggtca aatgtgtgca atcgccaaaa
     6241 gcaagcatgc cgatattggt tatgctgtct ggcagaataa ttgtcgtcag attggtacag
     6301 ccatagaacg cattgccatg aatacttgtc acactatccg gcacgacaac acttgtcaag
     6361 gcagtacaat tgttgaaagc acctatattg atcgtcacaa catcttcagg aattacaaca
     6421 tcaccagcac aagtacttcc gtcaatcaaa agatgattga taattaccaa tggattttct
     6481 ttctgtcttg ctgccagcca agcagtgccg taaaaagctt tgttctctat actagtcacg
     6541 ctgtctggta tcgtaatttc agacaaattg gtacagtttt caaaggtgtt atgtgcaatt
     6601 gtagtcatgc cactcgggag agttacagca gtcagaccag tgcaattttc aaaagccaac
     6661 cctgctattg cagtaacgct atccggtatt gtgattttct tcagtccagt acaattcaaa
     6721 aaggcactag ttccgacact tgtcatgccg tccggcagca caacttcagt cagacctgca
     6781 cagtccacaa aagcatactc atctatactg gtcaggctgt ttggcatggt aatcttttct
     6841 aattttgtgc atgccttaaa cgcataacgt ccaatgtttg ttacgctatc cggtatagtg
     6901 atttccgcca aactggtaca gttagaaaaa gtatattctc caatacttgt catactgttg
     6961 gatagggtaa catcagccaa atttgtacaa tctgaaaaaa tgatttcctc catgcttgtc
     7021 acactgtttg gaatcgtgat ctctgtcaaa ctggtacaac cagaaaaggc agcttgtcca
     7081 atgctcgtta cgctgtccgg tatggtaatt gcagacaaac tggtgcagcc cgaaaaagta
     7141 tatcctccaa cactggtcaa gtgatcggat agtgtaatgc tttccaagtt ggtacaattc
     7201 gaaaaaacaa aaccgccaat atctgtgaca ctgttcggta tagcgatctt tgtcagactg
     7261 gtacagccgg aaaaagcact ttcaccaatg cttgttacgc tgtctggtat ggtgattgca
     7321 gtcagattct caagatttga aaatgcattt ccttcaaagc ttgtcacact atccggtata
     7381 gtaatcgcag tcaaatcact gcacccataa aaagcatttt ttgcaatact tgttacgcgt
     7441 tttccgtcaa ttcttgccgg aatttctact gattctacag tttcattgca gccggaaatc
     7501 tttacttcat cggtatctgt cacgctatag tacaagtccc cgtaggtgcc gtcataagaa
     7561 tttatcacat atgcacttgc ggtgaatact gtctccacgc tctccaacat gttctgcacc
     7621 ccagacacgc caacacnnnn nnnnnncgcc aacactaccc agacagagca cgccggcagt
     7681 cacagctgca agcacttttt tccacagctt catcttcatt cctccgtttt ttcttttgtt
     7741 gcaaattgtc tgtttttatt atagcatgtc aacttaaaaa agtcaaatgc cttttcataa
     7801 atttccagtt ttgtggaaca ttctgctatt gtccaagtga tttttccatg tcttccgcga
     7861 atttcccact tcgaattctt ccaaaattcc aacaaatata caaaaagtca gctttaaatc
     7921 aaacctgcat tgtcaaaaac agagaaatca ttacaaatat attgcatcgt tttcaatgac
     7981 gtaatataat aaaagcagga aaattctgcc ggaaacccgc agaacatggg cagctgtgtt
     8041 ctgctttttc tttccctgga atttgtcgaa tcgtgtcgaa aaaaggagaa tccaccatga
     8101 aaaccgcatt ccaaaaaaag gcatatagta tcctgacggc actggtgctg ctgctgacag
     8161 catgcaccgg tgccgttttc agcagcagtc gggcagcggc tctggaactg gagggtacag
     8221 gcaaagcgga caatccttac ttactctcaa ccgccgcaga actgctggag tttgcggaaa
     8281 aggcagcggc tgatcccagc atttgtgcaa tgctgacggc ggatatttcg ctggaaggag
     8341 aaacttggac accgatcggc tcctatgccg gcacttttga cggcaattat cactgcattt
     8401 cgaatttaca gtgcagcggc ggcagaaaca caggtatgtt taccaatctg gaaggcactg
     8461 tgcaaaatct ggggctgact gatgtgcata tccagggaaa aaactatgtc ggcggaattg
     8521 cggcagtgtg cagcggtaaa attataaatg tattctgcga gggggatatt actgcaacaa
     8581 gtagtgcagg agctgccggt ggtttaatag gacagggcaa aaggaaatac taccaaggag
     8641 ctgttttgca aaacgcctac cacatcggca cagttaccgc aaagggcacc gtcggcggtc
     8701 ttgccggacg aagtgatgca ccgttgcaaa actgctacca tatcggaaca gttgcaaaaa
     8761 cagacgctgc ttccgaagat tccgccggca tgctggtggg cagactggga gagatgccag
     8821 aaacgccatt tcagaattgc ttctacacag cgggaaatta ttctgcggtt ggacaaggtt
     8881 acgcagaacc agagggcatg cagcaggcag aggagaattt ccagaacggc attgttgccg
     8941 aactgatgaa tgccggcggc gatacgtggt gtgtccgcga ggggaaaact gtactgaaag
     9001 aattcgcgaa caatacttcg gcagctacca ctacaatgga gggaactaca acttctgata
     9061 caacgagcac gactacttcg acaactacaa cttccactgc tacaagcaca accacttcga
     9121 caactacaac ttccaccacc acaagcacga ctacttcgac aacgacaact tccgccacca
     9181 caagcacaac aatttcaaca gccacgactt ctactactac aagcacgacc actttgacaa
     9241 cgacaacttc caccactaca agcacgactg cttcgacaac cacgacttcc accaccacaa
     9301 gtacaacagc tttgacaaca acttctacca ccacaagcac aatcactttg acaacgacaa
     9361 cttccaccac cacaagcaag accacttcga aaaccacgac ttccacaact acgaatacaa
     9421 cagcttcgac aaccacaact tccgatacta caagtacgac tgctttgaca accgcaactt
     9481 ccacaactac aagcacgacc acttcgacaa ctgcaacttc taccacatca aactcaacag
     9541 tttcgacaac tgcaacttct accaccacaa gaacaacggc ttcaacaact acgacctcta
     9601 ccaccacaag aacaacggct tcaacaacga caacttccac caccacaagc acaactgctt
     9661 caacaaccac gacttccaca actacaagta cgactgcttt gacaaccacg acttccacta
     9721 ctataagcac aactgcttca acaacgacaa cttccaccac cacaagcaca actgcttcga
     9781 caacaacaac ttctaccacc acaagcacaa ctgcttcgac aacgacaact tccactgcta
     9841 caagcacaac cacttcaaca actgcaactt ctactactac aagtacaacc actttgacaa
     9901 cgacaacttc cgccaccaca agcacgacca cttcaacaac gacaacttcc accaccacaa
     9961 gcacaacggc ttcaacaatc gcaacttcca caactacaag cacaaccact tcaacaacca
    10021 cgacttccgc tactacaagt acagctgctt cgacagccac gacttccact actacaagca
    10081 caaccacttc aacaaccacg acttctgcta ctacaagcac gacaacttcg acaaccacaa
    10141 cttccgctac tacaagtgcg accacttcaa caaccacgac ttctgctact acaagcacaa
    10201 tcacttcgac aaccacaact tccgctacta caagtgcgac tacttcgaca accacgactt
    10261 ccgctactac aagcacgacc acttcaacaa ccgcaacttc cactaccaca agcacgacca
    10321 cttcgaaaac cacgacttcc gctactacaa gtacgaccac ttcaacaacg actactttca
    10381 ccacaagcac gaccacttcg acaacgacta cttccaccac cacaagcaca actgcttcaa
    10441 caacgactac ttccacaact acaagcacag cggcttcaac aacgactact tccaccacca
    10501 caagcacaac gacttcaaca accacaactt ccaccacaac caccataccc cctgacgaaa
    10561 atttcccgca gatcacaggc gatgcagacc gcaatggtcg ggtggactcc acagatgttt
    10621 acatggtaat gctgtattgt gcatatcacg gtgccggcta tcacgattac accttcacgg
    10681 aatatccgga gatcgaagcc gctctgctga ccaagctgga cgtcaacgga gacggctcca
    10741 ttgacactat cgatgtctat tttatactcc gctacgttgc ctatcaggga gccggcattc
    10801 cgacagactg ggacgaactg tacgaaaagc atattttgca atattgccca gaatattctt
    10861 tagatgtttt taaattgtac ccacaatctt tttacatcac cacacagaaa aagtgtataa
    10921 taaaataagt tgtaaacaaa aaacgaaaaa caagaaagga caagcaaaca cacatgaaaa
    10981 aactaattac acttctgcac ggtttctgta tggcactggc agacagtgtc cccggcgttt
    11041 ccggcggcac gatcgcattc ctcatgggct tctatgacaa ctttatcggt tccctgaacg
    11101 ccctgatctc cggcagcaag caagaacgca tccaggctgt caagtacctc atcaagctgg
    11161 gcatcggctg ggtgatcggc ttctgctcct cggtgctggt gctggcaaat gtctttgaat
    11221 cccatatcta tcgggtatcc tccctgttca tcggattcat catcttcgcg attccggtcg
    11281 tgatctacga ggaaaaaaag gtactgaaag accgctggaa aatgagcttc ttcgtcctca
    11341 tcggcatggc attcgtggtg gcgatcacct atgcaaacac ttttgtaggc ggcggaaaag
    11401 atctgagtaa tcccgacatc agcatgtaca tctacgcatt cttcgtggga gccgttgcga
    11461 tctgtgccat ggttctgccg ggcatttccg gttccacgct gctgctgatc ttcgggctgt
    11521 acattccgat cattaacggc attcacgacc tgctccactt gaatttccac atgctgccca
    11581 ttctgatcgt ctttgggctg ggtgtcatta ccggcatcat cagcattatc aagctgatcc
    11641 agaacgccct gaccaaacac cgcggggcta cggtatatct aatcatcggc atgatgctgg
    11701 gttccctgta ctccgtcttt atggggccca ccacgctgga aaatccgcag ccggcaatgg
    11761 gactgcacac gttcagcatt gtgttcttcc tcatcggtgg cgtagtaatt ctgggcatgc
    11821 agggcttgaa aatgctctcg gataagaaca gcacccctgc caatgagcca aaagcagaag
    11881 tgcaggcaaa gtaaaggcaa taccgcacaa agagcgtctg gcgactttgc acgccatctg
    11941 cttgc
//
LOCUS       DAWBGR010000068        11880 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1620_length_11880_cov_5.861734, whole genome shotgun sequence.
ACCESSION   DAWBGR010000068 DAWBGR010000000
VERSION     DAWBGR010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11880)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11880)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11880
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1620_length_11880_cov_5.861734"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7011..7020
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3018 a         2694 c         3244 g         2914 t
ORIGIN      
        1 ctttgtgcgg tgttgcccaa ggaacaattc ctgcaccggc agtggggaaa gtttatgcgg
       61 cgaaaataat ggaggcatag aacgtggaac atcagaatcc tttttcctat gcggaattcc
      121 tgcggatctt tgaacatgac gactggtttt tgattgatga aacggtgttt tatctggact
      181 atgctccgga cgaagaatat tatttgggtt gccttcggga atacgaggaa ccgtattggg
      241 cagggtactg cgatatttcg gaggacggct gttttcgtac agcttcggca ctgttgaatg
      301 caaaaatttt tcatgggcga tctgtcaaag aacgttggga aaatgtgaga ttttttcaga
      361 tcggcgggat tcccgtggaa acatggctgg aactgtatga agaagatttg ccgaaagtgg
      421 aacgggaatc gcgaatcgaa gaactgtacg gggaattttt gctgtggaac tgcgggtttc
      481 attccagtga aacttatttg tccatgctgg atacgcttct tttggagtac cccgaaaacg
      541 ccttactgct gaagctgaaa aaattttctg aaagccgcaa agtcacttgt cgtttgtttc
      601 tgcaccactg gaactatgag agcgtgtctg ccggacgtgc ggattcttcc agttatgctg
      661 cttttgggaa atatctgttt tccgcattgc aaaagcaata tgagggaagc caatttaacc
      721 ctgaaacata cagcatcggc tgcttttgcc tatggcatta cctgccggaa gcggtcaagg
      781 caaaagaacc gttttctacg ctgtgccgcg tgaaaacgta ctgggaccgc aacgatacac
      841 aagcttggaa attgctacag caggcgtttg cattttatga ttcaggcaac actgcataac
      901 gcttgtgagc aagatacgtt gtcagattgc gtgcaaagcc tgttgcctga aacacgtttt
      961 gtgaatggag gaacagtatg aaacattgca ggattccgat tcagcggatt cgccccgcgg
     1021 cattgcattt tatgagagaa acgggttccg tctgactgga gaaaaaagaa tcgaagatga
     1081 agttatttcg ctgctgaaaa tggagcgtgt cacagaataa aaaggagaaa cacatatgac
     1141 caaaaaagaa cgtgccctgc tggcggtgca gacgctggaa gaagtttatc cgcaggcaac
     1201 ctgtgcactg caatatgaaa agccctatga actgctgatc gcggtgcggc tgtcggcaca
     1261 gtgtaccgat gcaagggtaa accagatcac accaaaactg ttcgcagctt atcccacact
     1321 ggaatcactg gcggcggcag acatcggcga tttggaagag atcgtccgtc cctgcggctt
     1381 ctatcacatg aaagccagga gcatcaagga aatgtctgcc cagctgctgc tgcacttcgg
     1441 cggcgtggtg ccggacaata tgaaagatct gctttccctg tccggcgtgg gacgcaaaac
     1501 tgcaaacctg attctcggcg atgtctacgg caagccagca atcgtggcgg atacccactt
     1561 tatccggatc accggacggc tgggactgac caaatccaaa gaccctgtac aggtggaaaa
     1621 agatctccgc ccgctgatcc cgccggagcg ttcctcggac ttttgtcatc gggtggtgtg
     1681 gttcggcaga gatacctgcc gtgcacgaaa gccccggtgc aagagctgtc ccatggcggc
     1741 attctgtccc tcccgaagcg agtaagggcg agttccgcga cagtcggcag gggaaatgtt
     1801 gcggaatgca cagagaatct gcttgaaaat gaattgcact ttgtgaaaat atttcaaaat
     1861 ctattgacaa gcagagtggt tcgtgctata ctagttattg tatatttcga tgaaaaggcg
     1921 gtaagtcaat gggcattttt agtaaagtat tcggttcgta cagtacccgt gaactggcac
     1981 gtattgaacc gctgaaaaaa aaggttctgg agttggacga ggagtatcag gcactttctg
     2041 atgcagatct gaaagaaaag accaatgaat ttcgggaacg tctggaggac ggcgaatcgc
     2101 tggaaagcat ggaggcagaa gcactggcaa ccgtgcggga agctgcatac cgtgttctgg
     2161 gcaaaaaacc gtttccggta cagatcatcg gtgccattgt cctgcatcag ggacgtatcg
     2221 ccgagatgaa aaccggtgaa ggtaaaacac tggtagcctg tgtggctgca tatctgaacg
     2281 cactgacagg caagggcgta cacgttgtta cagtcaacga ctatctggca aagacacagt
     2341 cggacgaaat gggcaaggta ctgcggtatc tgggcttgac tgtcggctgt attctccacg
     2401 gtctgaccaa cgaccagcgt cgtgcggcat ataactgtga tgttacgtac ggtaccaaca
     2461 acgaattggg atttgactac ctgcgtgaca acatggtcat ctataaaaag gataaggtac
     2521 agcgggaaca caacttcgcg attgtggacg aagtggactc cattctcatt gatgaagcca
     2581 gaaccccgct gattatttcc ggtaagggcg acaagtctac tgctctgtac ggcgtggtgg
     2641 acaagttcgc caagactctg accgctgcaa ctgtcgtaga aatggacgac aagcaggatc
     2701 aggaagaagt caacgaaaac gccgactaca ttgtagacga aaagtcaaag actgcaacca
     2761 ttacccgccg cggtgtcaag aaggcagagg aatattttgc agtcgaaaac ctgatggatc
     2821 cggaaaacat gaccttgttg caccatgtca atcaggcact gaaggcaaac ggtgtcatgc
     2881 ggaaagacat tgactatatc gtagaggacg gcgaagttgt catcatcgac gaattcaccg
     2941 gtcgtaagat gctgggccgt cgtttcaatg acggtctgca tcaggcaatc gaagccaagg
     3001 aaggtgtaga ggtcgcacag gaatccaaga cgctggcaac catcaccttc cagaactact
     3061 tccgcctgta caccaagctg tccggtatga ccggtactgc aatgacagag gaagacgaat
     3121 tccgtgagat ctacaagctg gacgttatcg cgatcccgac caaccgtccg gtacagcgta
     3181 tcgaccacac cgacctgatc taccgcactg aaaagggcaa gtataaggca atcattgagc
     3241 agatcgctga gtgtcatgaa aagggacagc cggttctggt aggtaccatt tccattgaaa
     3301 aatctgaact gctctccagc atgctgaaac gaaagggcat caagcacgaa gtcctgaacg
     3361 caaagtatca cgcaaaggaa gcagagatcg ttgcacaggc tggtaagctg ggtgcagtta
     3421 ccattgccac caatatggct ggtcgtggta cggatattat gctgggcggt aatgccgaat
     3481 tcctcgccaa ggcagaaatg cggaagcggg aatatccgga agaaatcatt acagaagcaa
     3541 tcggctatgc agatacagac aatcaggaag tgctggacgc aagagccgta tatcaggaac
     3601 tgtacaaaca gttcggcgaa gaagtcaagg aaaaggcaca ggcagtcaag gaggctggcg
     3661 gtctgtatat tctgggtaca gaacgtcacg aatcccgccg tatcgataac cagctgcgtg
     3721 gacgttccgg ccgtcagggt gacgagggtg aaagccgttt cttcctgtcg gtagaagatg
     3781 acctgatgcg tatttttgcc ggcgatcgtc tggaaaacat gatgcgtaca ttgaacgtgg
     3841 acgaggatac cccgatcgag agcaaggctc tgaccaagat catcgagtcc tcccagagaa
     3901 aggtagaggg acggaacttc aatatccgta agaacgtcct gaactacgac gacgttatga
     3961 acacccagcg tgagatcatt tataagcagc gtgcacaggt actggacggc gaagacattc
     4021 acgacagcat cctcaagatg ttcgaggaac tgattgccgg aactgtaaag cagtacatca
     4081 acgaagaaga aaccgatcac aaccagtgga atctggttgg tctgcgggat cacttcctgg
     4141 gctggatcac agaagaaggc gatctggaat atgatgaaac cgatctggca ggtctgacca
     4201 ctgcggacat taccgaggct ctgactgaaa aggcaaagtc tttgtatgct acaaaggaag
     4261 aagaattcgg ctctgaagta atgcgggaac tggaacgcgt tgtactgctg aaggttgtcg
     4321 ataccaagtg gatggcacac atcgacgata tggacgaact gaaaaagggt atcggtctgc
     4381 gggcatacgg tcagcagaat ccggttgtcg aatatcgtta cgagggcttc gaaatgttcg
     4441 atgcaatggt agattctatc cgcgaggaca ctgtccgcat gctgctgaca gttcgccttc
     4501 agaagaatca ggctccggag agagaacagg tttccaagcc ggacgctccc aatgcaggag
     4561 caggcgacgg cagcttcggc ggtcagcgtc gcagtgcaaa gaagaagtaa gaatgtttcg
     4621 ggaaatgcgg cacacgatct ggtgaaagtg tgctgcccta ccaaataagc aaacgaaaac
     4681 agggggacat ggcattcgct gtgtgccctt gttttgtgtg gaggtggatg catgcaggaa
     4741 acgggctatg aaacattttc gttttactat gatgccctga cggaaaatgt caactatccg
     4801 gcacgggcgg cgtatctgga cggactggtg cagaagtaca aacagtctgc cggcaaagtc
     4861 atgctggatc tggcatgcgg caccggcacc atgacagaag aaatggcaga acgtggctat
     4921 gacatgatcg gcgtggacta ttccgagggc atgctggggc aggcaatgga aaagaaaatc
     4981 gaaaagcagc tgccgatcca gtatgtttgt caggacatgc gggaactgga actctatggc
     5041 accgttgatg ccgtgatctg tacgctggac agtctgaacc acctgccgtc gtttgaagat
     5101 gtcacaacgg tgtttcagcg tgtgtgggaa tccacggaaa ccggcggcgt gtttgtgttc
     5161 gatatgaaca cgctctataa gcatcgggaa ctgctgggaa atcaggtcta cgtctacgaa
     5221 acagatgcgg tttactgcgt ctgggaaaac gcgttgtgca aggacaactg caccgtggag
     5281 atcacactgg atctgtttca gctttgtgca gacggtcgct atcggcggac agaggagcag
     5341 atcaccgagc gtgcttatgc accggaacag gtgcaggcgg cactggaaac cattggattt
     5401 cacgtgctgg gcatttatca tgcagataca gaagaaccac tgcattcgga cagcgaacgc
     5461 atggtggttg tggcgagaaa ggaagcataa acaatgggaa ttctgaaacg tgcaattgca
     5521 aaagatgcat cagtcgtggc aactgcggta gatgccacgg atattgtcgc agagatcgaa
     5581 aagattcacc agacatctgc ggtagttacg gcggcactgg ggcggctgtc cattgcggca
     5641 agtatgatgg gctatgacct gaagaatgag aacgatactg tcaccctgcg tgtcaagggc
     5701 ggcggtccgg caggcatgct ggtagctgtg gcagacagtc ggggaaatgt caaggcagac
     5761 gtagatcagc cggtggtgga actgccgctg aatgccaagg gaaaactgga cgttggcggt
     5821 gccgtaggta cagacggtac gctgtctgtg gtaaaagatt tggggctgaa agagccttat
     5881 gtgggcattg tgccgctggt gtccggtgag atcgccgagg acgtggcaca gtattttgcc
     5941 actagtgaac agacacctac ggtttgcgga ctgggcgtgt tggtgaatcc ggatctgaca
     6001 gtcaatgttg ccggtggctt cctgatcgaa gtgctgcctt ttgccagcga tgcctgcatt
     6061 gataccattg agaaaaatct gaaaacactg ccgccggtaa ccactatgat gacacagggc
     6121 atgacaacgg atcagatcgc tttgcggctg ctggacggac tggaaccgaa cctgctggac
     6181 gagggcgaag tgcactacca gtgcgactgc aatcgggaac gaaccgagcg tgtcctgaaa
     6241 gcactgggca atcaggaact gacacagctg atcgcagaag gggaaccggt agaggtcaac
     6301 tgccatttct gcggcaagaa gtatacgttc acaccagaag aattgcagca gctgcgggac
     6361 gaggagccgg aagaataacg tttctctaag gcaataccgc acaaagacgt gtctgggaaa
     6421 ggataagagc atgcggaact ggaaaaaaat cgtgggattg cttgtcgtta tggcagtgtg
     6481 gctgggattg atgtggcggc tgtccaccgc agacggaacg gaaacgctgc aggacagcat
     6541 gcgttttgcc cgaaaaatcg gcagctggat ctatgaatct cctactgtac agcagctgaa
     6601 ccatctgaac ctgctgctgc gaaaactggc acatgttttc ctgtatgcga tactgggcgg
     6661 catgatggct ctgctctggc agctgcttct ggaactgcat cgaatcgggt ggcgtattct
     6721 gggagcggca gcttgcagca cgacaattgc gtttttggac gaactgcaaa aaatcccgat
     6781 tgccggacgg cacttcgact tttcggaatc gctgctgaat gccggcagtg cccttgtggt
     6841 cattctgctg tttttcggaa ttgccggact gctttcccga aagaagtcaa cttcataatc
     6901 tgaaaccgaa agcattggaa ttgcaaaagt gatttgcggt tctgatgctt tttcttctct
     6961 gaaaattttc aaaaaatact tgaattttcg tcttgcattt tgtataatat nnnnnnnnnn
     7021 aaatgctttt caacttttga ggaagaaagg gtcgattcag tatggaaaca caggaagctg
     7081 gtttaaaaac cgtggaaaat gcgtatatgg aatccagaag aatcctgcgg acaccctcgg
     7141 cattggcacg ggaggctctg ctgtatgtgc aggaaatcgg agaaatgact tgtaaagagc
     7201 cgcgtcagaa ctggcggaat tcgttggatt cgtacctgct gattctggtg gaagaaggct
     7261 gcggaacagt taccgtcgga gaggaaactc ttgccctgca agagggggac gtggcgtttc
     7321 tggactgcca ccaaccctat tctcattgct gtgatgccag agaaccgtgg aaaatccttt
     7381 gggtacactg gaacggacag gtaatgccac atttatacga ggtctttcgg cagcggaatg
     7441 tttcagttgt tctcggcggt gcagcagact gggtagaacc tgtgctcctg cgtttggaat
     7501 ctattgcaag agaagaagca ccggattatg aactgtatga atccgagtgc ctgactcagc
     7561 tgatgacgct gttgctgacc aaaaaaccgg aacgcggcgg gcatgcttcg ggggaaacac
     7621 tccagaaatg ggaacaaatt catcagtatc tggaagaaca ctttgcagaa aaaatcacac
     7681 tggaggatct gggaaagcgt ttcagtgtga gcaaatacta tatgctgcgt ggattcaaac
     7741 ggcggtacgg cgtaacgatc gtgcagttta tcaaccagtg ccgtatgaac tacgccaaaa
     7801 acgaactgcg atttacagat aagcaaatcg acactattgc agaagagtgc ggcatacacg
     7861 attccagtta tttcaatcgg attttccgta ccacagaggg gatttctgcc ggagcttatc
     7921 ggcggcagtg gcggaactaa aacataaaaa caaacgggct gcggcaattg ctgcaagccc
     7981 gtttttgtat acacaccaaa aagaaaagga ctgccctgca atgggacaat ccttttcctt
     8041 tataatagga gtgtagaaaa tatcaaaatg gcattactga ggatttaccg gcagagaact
     8101 tatcagctgt accaggaact ggagcaatgt agttgcatca tcgccgttga tctcgccgtt
     8161 gaacatgcag tctgcgtttg cagcagcctg tgcgttcagg tctacctgac cagctgcctt
     8221 cttgttcagc agaaccgcat ctgtaatgtc taccttgccg tccagattga cgtcgccgta
     8281 gagcgatgca ccgataaccg gaacttcgcc gctgtcgctg gctgtggtag aagccgcagt
     8341 tgttgtggtt tcatcagtgg aaacagctgt cgtatccgag gaaacatcag tatcggattc
     8401 ggtggttacg actgtggttt ctgtatcggc aacagttgtt tctgctgtta cggtagttgt
     8461 ttctgtatca gaaacagttg ttgcctctgt tgtggtttct tcagaagaaa ttgtagtagt
     8521 tgttccagag gactctgtag aagtgctgct gctttttgga agatatgctc cagtcagctt
     8581 gacgttggag gattccaacg tatcgccagc accattagcg acccaccaga tctgtacttg
     8641 tgcattcttc tgcttgattg cttcatcgga gatcttcttg atcatgtcgg catcagtaac
     8701 ttcgtccttg ccgttgttgt aggtgatatt atacggtttg tccagttttg cagtgacctc
     8761 gtcagtggag tccagatccc atttgtagtt cagccagtaa tccacgccgt ctacattagc
     8821 agaaataccg atgcagccgt ttgcttttga aacgtcatcg ctcagatcga atgtcagatc
     8881 cacgctgtta ccaatggact gtttgaatga aatgttgtaa ttgccgctgt cagccacgat
     8941 ctttgcagct tctcccttgc cttccggtac ggtagttgtg gtagtaggct gtgtggtgtg
     9001 gctgggcttg gtgccggtgc cctgctgacc attgccgtac aggtcggtcg gcagttcgtc
     9061 cagtgtaatg cagtagtcac tggtgtacat ttccttcagg tcgtcctttt tgttgaacag
     9121 ttcatttgtc aggaagttgg tgctttcgct gccgccgtca taccacgggc agaaatacag
     9181 ccagccagcc ttttccgata ccagattgtt cagcgtcgga atgctgtcgt tttcagccat
     9241 agctaccatc ttcttgcagt tgtacttctg cataatggag tagaaggtac tgctgatggc
     9301 actgtcatta tgtgtcagaa ctgcactgcc ggtagaccag tctgtgcagt tgtacttgtc
     9361 ataagccacg atgtctacat attcatcgcc cggataccag tttgcggaag tgtcatatgc
     9421 ataactgttc cactcccaga tcaggttgtg cagaccgtac tgttcggtca gggtttcata
     9481 ggtgaggatc cacagttcct tgtaaacagc ggagccttcc ttgccccacc agaaccagga
     9541 accggtttca ccgccgccgc cttctgcttc atgcagcgga cggaagatca gcggaacgtt
     9601 tgcgtcctgc aatttctgca attctgctgc cagacctttc aggcagagca tatagtattc
     9661 ccgttccttt gtgccttctt ccagaatctt ggacggctca aagtcggttt ccttcggaac
     9721 ataggtcgca tttgcccagt ctaccttatc gccgatgttg tagctggaga aatccttcgg
     9781 tacagtgatg tgccaggaag aggttgcgat accctgattt tccgaatagg ggttgttgtt
     9841 gacccactga atgatgcggt ctgtagtgcc gtcttcgctg ccgtacagcg ggttgcagtt
     9901 cagatagtca aagccgcgga ttgccggata tttaccggtg gtgtcgttga tgtacttgaa
     9961 ctcatattca aaatcgtggg ggccatactt gtaaatttcc tgctgtccgg aaatgatgtg
    10021 ctttccgtag acgctggaca gatacttcat caggctgtcc gcttctttgg ttgccgccgg
    10081 atcgctgcag gtggtgtcac ttgcggtgat gtccggcaga gtagcttctt ctacagtgag
    10141 atagtcgaag ttggtccagc cccagctgcc cttaatggca atggtgttgt caccggcagt
    10201 cagcttgaca gtacccagat ccagttcttt ccacacgcct tctgcggtag atgcaaagct
    10261 gcactgtccc tgatcgacac cgttgacata caggttctgg atcttgtcgc catagggagc
    10321 agaatagctt acctttaccg tgtacatacc agttttttct gacggaacag tgagagaaat
    10381 ttcatcaccg ccggttttca gaaagacata gctgccgccg gaagcgttgc tgtcatcggt
    10441 ttttacttcc gcgtcaccgg tgagcttacc gctttcaaat tcgtacttgg cagttgctgc
    10501 accagcttgc agaacactgg acagtgccag acagttgaca gacattacgc cggcaactgc
    10561 ggtagtcagt gctcttttga gatttttctt cataaatctg atctccattc tgctgcgttc
    10621 agcaagaacg ctggtttcat tgctgccgca gtgcattttc tttttatgtt ttcatagtga
    10681 taaagtacag attcggagcc gcccagcgat gcgactgtcc acgtttaatg cttattatat
    10741 cacaagtatt gtgcaatgtc aatagtcctt tctgtacatt ttcagaaaaa tttatgcaca
    10801 tagagattgc tcttcacggc aatataagaa cgttctgaaa agattattga aaaaaataat
    10861 gccttggctc tctgcagagg atttttgtaa agctatgtga aaaaatgatg aaaacagaaa
    10921 aaggaagtgg aaaaaggctt gcttttcccg ccgtggtgtg ctatactata ggcaatacca
    10981 cataaagagc gtctggcgac tttgcacgcc atctgcttgc aaatttctcg tcatatttca
    11041 caaaaatgct cgtgaataca tcaagtattc actccgcttt ttgtttcatc tgacgaaaaa
    11101 tttgacgacg catctgtcgc acaatctttg tgcggtattg cctataggca gaatcaacga
    11161 taggagtgag gcgtatgcgg catcggatac agcactggga acagcagacg ctgctgtggt
    11221 ttcaggaacg cctgcggcgt gcatggctga cagcggcgat gaaaacggca acatttttgg
    11281 gaaacggtgg aatcctctgg ctgacggcgt gtgcctgcct gctgtttcgc cagcagacaa
    11341 ggcgtgcttc tctgacggcg ttgctgtcac tggtgttttc tgcattggtc tgcaatgctt
    11401 tcttgaaaaa tctggtggag cgtgccagac cgtttgacaa gattccaagc ctgcaattcc
    11461 taattcggaa acctcacgat ttttcatttc cctctggtca tacgtccagc tcgtttgcag
    11521 tagcgactgt gtttctggca atgctgcccc tgtgggtagg tgttgcggca ctgacaattg
    11581 ctgtgctcat cgccttttcc cgtatgtatc tgggtgtgca ctatccgtca gatgtgctgt
    11641 gcggtgcggt gctgggagtg ctgttcggat tggcagcctt ggcagtgatg ccgctgctgc
    11701 ttcgcatcag ctggctgcca gaagcagtgc gtacctgggt tggggcataa actgaacgct
    11761 ttttcatctt cgaataaaac agcggatacc cgttttcgaa tcgggtaccc gctgtttttt
    11821 ctgtgtttta aggcaatacc gcacaaagat tgtgcgacag atgcgtcgtc aaatttttcg
//
LOCUS       DAWBGR010000069        11872 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1621_length_11872_cov_7.320809, whole genome shotgun sequence.
ACCESSION   DAWBGR010000069 DAWBGR010000000
VERSION     DAWBGR010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11872)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11872)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11872
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1621_length_11872_cov_7.320809"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9247..9256
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2867 a         3258 c         2765 g         2972 t
ORIGIN      
        1 aaattcgcag gcgtgctttc gcacggcaag gatttttaac gacgaacggg gaaaagtcac
       61 cccaaaagac acgtgtcagg cttagtgtca acacacccta aattatcagg tacagtggcg
      121 gaagttcttg tcggtgcaaa gtcttgcagc caccaagccc atcggcaatg ccataacgat
      181 cagaatcgct ttggtcagcc agagagagcc gtcgccgatc tcattcagcc cgatatagta
      241 gataccacgg tacatataca agcccggaac catgatgacg attgccggta cagtcaggga
      301 aattcgcgga taaccaatct ttttcttgac gatcgatgcc acacagccgg tgaagaatgc
      361 tccgataaat gcagcaatgc ctaccgggaa atgtgccaga tcgatcagtt ccagacggag
      421 cgtgttggca aacatgccga tcagtcccgc tgtcattgcc attttccgcg ggctgttgaa
      481 catcagagag aagccgtata ccccgaaaaa gctggtgatg attcgcaaaa taatcatcat
      541 gccggtgtca atttgcagag ccacaaagtc tgccggctgg aagcgacaga ccagagccgt
      601 cacccaacca gtcatggtcg cagtgaggat aatcagcaga gcgtatgtga tccgctccag
      661 accggaacga acatccagct tggcaaggtc gataccgccg gtaatcaacg ggaatccagg
      721 gatcacgaac agcattgcac agatatagcc cgcctgatgc actttcgaca cgccgaaaat
      781 ggtctgtgcc agcagaatgg tcagcacgta tgtgacacag gaagccgcta cgctgacagc
      841 aacatttccc agcagggaaa tctttttttg cagcatcagc tttcgcacaa agtttcccac
      901 gcctgcacca aaaaaggcac agatcatttc cataggaccg ccgcccagca aaaaggtaaa
      961 tgcacagcac gcaattgcgg atgccagtcc cagattccaa gccttataat tcgccggctt
     1021 tttctgaatt tcgtccaaca ccagatgaat ctgcttgacg gagatctgcg ttgccatttc
     1081 cggaaattcc cgcataaagg cttccagagc attcagctta tccgtattca cgccggtagt
     1141 cggcagtgcg atcgaatggg tatatgtgcc gctttcctca tagcaagtga actcaatgga
     1201 cagcagacca atatctgccg tacaggaaat gcccagcgtt ctggcaacct cattcatcga
     1261 attccgcaca cgccacgcac cggttcccac cgaaagcatc atctgtccga ttctacccac
     1321 cagcgtcgcc ttttccttta aggtcgcctc agtcgaaggc acgctgtcat catccttgac
     1381 aatgtgcttc cagtggatcg tcatgtgctg tttgttaata ctccccatgc actttgttcc
     1441 tcctcttttt tcatgcagca ccgcaaaagt cattcagcag actttctaca gtagtttttc
     1501 aaaggcaata ccgcacaaag ccttgtttct gccgctccaa cacaacagcc gcccgttttt
     1561 gtcggtgggc ggctgttttt tttccattgc agaataccct atttattata gtaaactcat
     1621 tttgcagtgt caagattttt ttcgtgaaca caggacagtg gggaacagat tttttgcaca
     1681 gtttttgtag aattttgggc agattgtaat ttccggaaag caaaaaagca gtttcaaaaa
     1741 cttgaaactg cttttcattc tggcggacag ggagggattc gaaccctcga tacgctatta
     1801 acgtatacac gagttccagt cgtgcgcctt agaccagctc aaccacctgt ccatttatag
     1861 tgcttcctgc gatttgagga agtggtgcga gtgacgggac ttgaacccgt acgccgaagc
     1921 acacgcccct caaacgtgcc tgtctgccaa ttccagcaca ctcgcttcat gttttttcat
     1981 gtgtcgcatt cagctttatt attatagcag aaaaacaagc ccttgtcaac acttgaaatc
     2041 gcttattttg tttatttttt gtaaatatag cgtgaatcct ggagaaaaca gcatttttct
     2101 actgcacatg caaaaaggca gaccctgccg gagcgttccg cacagagtct gcctatgaaa
     2161 tcttttacac ctgttccgtt tttgccgatc acagcatctt tgccacgtct tctgcgatct
     2221 gctctgcggt caggcggttt tcccgcagaa cttcctgtgc atcatagcga tccagaaatt
     2281 cctttttcag accatagcac ctggtcttta ctgcagagtc gccgtagaac cgtgcaatct
     2341 tttcaccgaa gccgccgtcc agcacgccgt cctccagcgt aatgactgcc ttgtgactgg
     2401 atttcagctg ttccagtagt tcggtgtcct gtccggtgat ataatacgga ttgacgactg
     2461 tcggcgtgat gccggtcttt tcctgaatct tttccgcagc agcaacgccc agcgaataga
     2521 acgagcccag tgccagaatt gccacatcgc tgccctgctg tgtcacctcg taccggttca
     2581 gctgactgaa atccttggtc acagccttgc cgtcggaaac atactgtgcc ggcacacgga
     2641 ttgccacagg gtgttccgtc tgatccagtg cccagtccag cattgcacgg tattcctcta
     2701 cagtgaccgg tgccagatat accagattgg ggatattgga aatcatggga atgtcgtaga
     2761 tgcccagatg cgtcacatca ttcatgccaa aaacagaagc ggcgaaaacc gcaatggtaa
     2821 cagcattctt gttgatgcag atgtcctgcg acagctggtc gtaggtacgc tggaggaagg
     2881 aactgtacac gccccagaac ggcttgccgc cgtttttcgc aataccggaa gccattgccg
     2941 cagcctgctc ctctgcaata cccacgtcaa caaactgctt gccagtcttt tcccgaatct
     3001 ccggcgtgaa tccgaacacc gccggcgttg cggaagtgat cgcacagacg ctgggatctg
     3061 ctgccatttt ttcctgcaaa tagtttgccg taaatgtgcc gtagtcgccg tcgccgtcaa
     3121 aggaaccggt gggctttccg gttgccggat caaacggcat gcaccagtgc cactgctcct
     3181 tgtgctgctc tgccagttcg tagccctttc ccttttgtgt aacgatgtgc acagccaccg
     3241 gcctggtgct gtccttgacc tgccggaatg cagcgatcag ggacgaaata tcgttaccgt
     3301 ctttcacaaa cacatagtcc agacccatgg cacggaacag attgcactct gccttgccgt
     3361 cagtatcccg cagcagcttc agattccggt ataagccgcc gtgattttcc gcaatggaca
     3421 tatcattgtc attgacaaca ataataaagt tggtgtccat ttcgccggca aagtccagac
     3481 cttccagtgc ctcgccgccg ctgagagagc cgtcaccgat caccgcaatg acattaccgg
     3541 tttcgccgtt cagatcccgt gcctttgcca gaccgctggc aaggctgatc gaagtggacg
     3601 tatgccccac gttaaagaaa tcgtgttcgc tctcctccgg attggtatag ccggaaacgt
     3661 cgtcaaagtg ttcttcatac agataggcat cttttctgcc ggtcagcatc ttgtgcggat
     3721 agctctgatg agaaacgtcg tagacgattt gatccttcgg ggaatcgaat acataatgca
     3781 tggcaatggt cgcttccacc atgccgaaat tcggtccgaa gtgaccgccg tgcttgctca
     3841 gacgaaacag cagagccttc cgcatttctg ctgccagtgc atgcatttcg tctatgttca
     3901 gtttttttac atcttccggt gcattgattt tttccagata cattatgatt cctcctgttc
     3961 gtgattcttg ggaaacgcta cgtttccttt gcattcctgt ttatcccatg tgagtacgcc
     4021 tgtccggaca gcttcctcat agcggctgat cttgtagttc agccgttcca gtgtcgtgtc
     4081 gatctgctcc cgctgttcca gcagtgtttc ccgctgatcc agcagcagct gtaaccgtgc
     4141 cggaatggtg tcatcgccct gctggcacag cttcacatac tcgatcatga cttccaccgg
     4201 cagtccggca tttcgcatac agagggcaag ctccacccag cccagatctt ccggctggta
     4261 gttccggata ccgctgaggg ttcgggtgac tttggggatc atgcccaccc gctcataata
     4321 ccgcagcgta tcctgcgtga tatgatactt ttcgctgact tccttaatgg tcaagggttc
     4381 tcacctcctg caattgtatt ataacacttg gagttggctc caagtcaata gggctgtgct
     4441 ggaatttaca ttttgtctac aaatacaaaa aagcagcatt gcaaccaact ctgcaatgct
     4501 gcctttataa taccacgttg tcactgcatc gaatcaatct tccacagacc gttgtccagc
     4561 acaatttgta ccgtcagcgt atccgccgca ccgaaattgt cgtactgcat cgaagcggta
     4621 aacgaactgt ctgtagcatt ggaaatggtc acctgttccg gatcgatcca ggtgaagcca
     4681 ccactggtgg catactgtct gccgtaaagt ccgccgttgg catcgatgta cagcggcttg
     4741 tctgtgccga gaatggcagc atagcggctg tcgatcatct tctgtgtcag agaagctgtc
     4801 atgtaggctt tcaggtcggc agtgctgctg aactgcgttt ttgtcactct ttcataggtc
     4861 acgccggact ttgtcgtctg tatggtatcg gtttcatctt tcggcaggtt gccgctgccg
     4921 atctggtcaa tgtagttcaa cgccccgaac agtgtctttg ccgcagccgc atattccgaa
     4981 gtgtctgcac cggcttcggg aactgttgtc gtttccgccg aagtggtttc cgctgcgggc
     5041 tctgtggttt cgtccggtgc cggaatggtt tccgcagttt ccgttgcggc ttccgtttct
     5101 gcggcagaag tttctgctgt ttcctcggtg ctgctctctg cggcggattc ttccggtgat
     5161 gcagttgtgg tttcggcagt gcccgaagaa gacagcgacg ggatctcccc ttctgtacag
     5221 ccagaaaagc cggtcagcac tgctgctgtc agcattgccg caatccaaat cgtgttacac
     5281 tcctttctca tgatcgattc cctccttggg aaagactacc ggcgaaccgg cagttttctt
     5341 atgatgttct ttcagttttt cattcatctg ttcggcagtt tttttataga tctgataatt
     5401 caacgccgat gtgaaaagat acactgccgc actcgtcagg cacattgcca gaactccgac
     5461 aacagaaagt tcgtaccagc cgtacagcca cccgcatatc agcaccccca gggtaatcaa
     5521 cacatagtgt actaccagcc gcagcatgcc ttcccacttc tccatgtctt tcggcggcag
     5581 caccaattct gtaacagcac ctgccagcac gccaatggac aaaatttccg gcagcaccat
     5641 atagggaatc caaaagtgca gaatctcctg atgtcccgac agacagtaga caccgtacca
     5701 gacaatgcag gcgatcaaag tacctgtcgc agataccgtt gtaatggaga gaaaccgttt
     5761 gaatctattc ttcatcgccg cacctcacaa ccccagccgc tttttcacag cgggaacata
     5821 ctgacgggag atcacgactt tctccccgtt ttgcaggatc gcctcaaacc gaccgctgaa
     5881 cgacggagca aaccgttcca cctttgccag attcagaatc atcgacttgg aaatgcggat
     5941 aaaatccgtt ccggcaaacc gctgttccag ttcgtaaagt ttctgcttca attccgatac
     6001 ctccgctttt tcatacgcat acacgcgatt gtccacagac tcaaaataaa acaccgatga
     6061 cggacagagc acactcaggc tcccgtcctc gtgatagcac atcagtttct cgtcctccgc
     6121 ctgtatcaca cgcagcagtt ccagcacacg ttctgaaagc tgattgacgc gaacgatcag
     6181 ttcttcttct ccgccctccg gcggcttttc aatgacaact ctcatggcag tttttgtttc
     6241 ctttccgcaa tcccgtgtac caaaatgtag catcccagca acagcagaat tgtcaggcac
     6301 agaaacaggt acatttccgg aactgcaact gtttttcccg caaattcagg atcacagtcc
     6361 agtgcttttt gtatgccgcg aaccacttct tccggtacgc cctcgctctg catctgctgt
     6421 aacacgccgc ccatggcgtg atttcgcatc aatgcggtgg cataggtgct tggcaaaaag
     6481 gaaagcacct tttgcagacc ggttccaaag ccggagatcg gcatgtatgc cccgcaaatg
     6541 aaaccgtagc cggcactgac aatagtaccc accgccgaga tctgcccctg tgtactcagg
     6601 aaaaagtgaa tcagtgagga aagtgccgtt ccgaacagca ccagaagcac cacatcgccg
     6661 gtaagccgca gcacgtcgcc ccacgtatag aaccagccca ccgcatggac gtagcccaga
     6721 cacagcccga acgccgcaag gcagacgatc agagaagatg ctaccgttgc cagaaaatag
     6781 ctgactggca gcactgccgg tttcagcgga gcgatcagca ggtctttccg tgcaccggtg
     6841 atcttgtcct gcaccatcag catattggaa cagaacggca ctgtcacgca gcacactgcc
     6901 agaatcgatg acagcagctg tgcccctact gccccgtcga tcacctgcct ggaaagagaa
     6961 aagccttccg gaaatcccgc agaaaagttg ttccgataga cattccccag aaacgtcaca
     7021 tacagcacca gcaaaatcgc cggcgtaatc agcgaggtga aaaacatcgc cttgtcttta
     7081 aagtacagct tcacattacg cagaaacatg gctttccaca ttgtcggcac ctcccatatt
     7141 ttttcctgtt acagcaagaa agacatcgtc cattctgccc ttgatgatct cgtaatccgt
     7201 gaacaattcc ggctgctgca aaatcagctg tgtggctgtg cgggtatccg gcacggcaat
     7261 ttgcaacgct gccccgactt tttcataagg cagtcccagt gctgccaccg cggattcctc
     7321 tgcgtgatac agtgtaatcg tatcgccggt gtagcggttt ttcagttcca gcggcgttcc
     7381 ctctgccaga attctgccct cgtccagaat gaccacatgg tctgcatcgg cagcctcctc
     7441 catgtagtgg gtggtgagaa ataccgtcat gtgctttttc tttcgcagtt ccccgatcac
     7501 ccgccagaca gattttctgg tttgaggatc cagccctgtg gtgggttcgt ccagaatcag
     7561 cagctgcggc tggtgaaaaa tcgcccgtgc aatgtcgatt cgccgccgct gtccgccgga
     7621 gagcttcccc acgcttcgtt tcagcaggag cttcagctcc agcagtttgt ccagttctat
     7681 cagacgtttc tgaaatgccc tgccggtaat gccgtagagt gccgcacggg ttttcaggtt
     7741 gtccagcacc gaaagctgtt tatccagcac ggaatgctgg aacaccacgc caaggctctg
     7801 cctgatctgc ggcatgtcct ccagtgtatc ctttccctga atccgcacgc tgccggcatc
     7861 ttgccgcagc tgtccgcaca gcatggaaat ggtcgtgctt tttcctgccc cgttgatgcc
     7921 cagaaacgca aacaattccc cctgtttcac ttgcagggac aagtcgttca ctgcgtgcac
     7981 agttccgaag cttttttgta aatgttgtat ctcgatgcag ttttccatct tcaccgccgc
     8041 cttcctttga gatttgtttc agctggttgt cttgattcag tgtttccttt attatactgt
     8101 ttttataaaa caattgcaag aaaaaaaccg taagcggctt cgcaggcgag gtaacatgca
     8161 aaaagagagc actgtgatct ctcacgatgc tctctttctt gaggcaatac cgcacaaaga
     8221 ttgtgcgaca gatgcgtcgt caaatttttc gtcagatgaa acaaaaagcg gagtgaatac
     8281 ttgatgtatt cacgagcatt tttgtgaaat atgacgggaa atttgcaagc agatggcgtg
     8341 caaagtcgcc agacgctctt tgtgcggtat tgccttaaaa ggctattgtt gattctgcac
     8401 agatcttgcc gacggtttcc ttatttctcc gacagcgatt tctgctggga tttcaccttt
     8461 tcatctttca gaatatactg cggtctgccc ttgacttcag agtatgtctt ggaaacatac
     8521 agaccgatca cgccgttgca gaacatggag atgcctgcgg taaagcagag aatgcatgcc
     8581 agcgacggcc agccggcaac cttgtccccg aacgccagtg ttttccagat gatcacaaag
     8641 atcatgataa tggcaatggc acagaacgcc agtcccagaa ttgccgacag tgccagcggt
     8701 gctgtggaaa atgccagtat gccgtcaatg gcatagacca gcagacccca gaacgaccac
     8761 ttggtagtgc ccgcttcacg attacggttt tcaaatggaa tccacttggt gttgaatccg
     8821 acccatgcaa aaatgccctt ggaaaaccgg ttgtactccc gcagggacag caccgcatcg
     8881 gtcatctggc gtgtcatcat gcggaaatct ctggctccgt ccatgatgtt gaccttggag
     8941 atcttccgaa tgaccttgta gaacattctc gcaaagaacg accggatctt cgattcgccc
     9001 tttcgggaaa cacgccgcga agccacacag tcatagtcgg tggtttgcag agtttcgtac
     9061 atctcgataa tcagttccgg cgggtcctgc aaatcggcat cgataatgac tacatagtca
     9121 ccggaagcct tttccagacc ggcaaacatc gctgcctctt tcccgaagtt gcgggaaaag
     9181 gacagatagt tcacccgcgg attctcggct tcctgttccc gcaggaacat cagcgtccgg
     9241 tctgcgnnnn nnnnnnattc catttcgaca ttgtgcttta cgtgcattac ctcatagatc
     9301 ttttcaattt ctttcagaaa cagcggcagc acttcttctt cattatgaca cggcacaatg
     9361 atcgaaatct tgtcttgttt ttgcatatat acaccttctt tttcgggttc agtacgtttt
     9421 ctctgccagc tgtaaatccg ggtgctctgc cagatattcc ggattgacgt aaatatccat
     9481 catatcctga cagccgtcat agtatacata gccttcggtt tgcagcttgc tgacttcctg
     9541 atcctcccat tcctcgttct gctcagtgcg gacaataacg aagtccgtaa aggaaggcgg
     9601 ttccacatca ttgctgtcca gcaggtaaat ctcatcccgc tgggcaaggt gaggcagata
     9661 gaacgaatca catgccacag aggcatcttc cggaatcgaa tcgatcagtt cgtccttgat
     9721 gtcatatcgt tcccgattgt tgtcgtacag ttccttggaa cccacattgc cgcccaccag
     9781 cgaagaaaag aacagcaggg cggagatgct catgaagatc ggcaggaatt ccttatgctt
     9841 gcccttgagt tcggcatagt tgatcattgc cgcatagatc aggcaggtgg tggtaccgaa
     9901 cacatactgg aagccgtagt cattggcata gccatagccc ggtgccagat tcatcagaat
     9961 aaacggagcc agcaggaaat aatgcgagaa gtgcttcgtc acgaacggca ggaagaacag
    10021 cgggatcatg atgatcagca tcagcttgaa atccttttcg tccacgcact gggtgataaa
    10081 gtacatcggg tcagtgataa cagtcttaat gatgttgccg aagcttgcac tcttgtcagt
    10141 catcagattg ccgtaggtac gggaggtgaa cacgccttct ccgtattttt ccatcagtcg
    10201 ggtcaccaca acgaagtaca caccggagaa gccggcgaca acggcaccat gataccgctt
    10261 ttccagacgg aaaatgcaga acagtcccag aagaaccatg tagatcgaca cgtcttcctt
    10321 gacgatcaga agcagtgccg tcatgatgta catcagaaca tactttttct tttccaccgc
    10381 atagaagaac cacatcagca gcatgggcag gaatgcgttt tcgtggaagt catagaagca
    10441 cggagcaatc aattccacgc agaacagata gaccacagag aagccgatcg ttgccatgtt
    10501 ggagaacttg aatttccggc aaatgccaac cagcggaatg acaccggttg ccaccagaac
    10561 ggcctgtccg atgagcaggg tatacggggt cgggaaaatg tagtagaacg gcagcagcgt
    10621 ataatagatc ggcgagaagt ggactgcaaa gtgagaaagc agcttgtccc gttcgcaggt
    10681 agtgaccggt gcgaagctct ctgccatgtt atgatacatc tgtacaaaga tgccgaagtc
    10741 aaatgccatg gagccgtaga ccttatgccg gtaaatggtg aacacggcaa tgaatgctcc
    10801 catcaggcac atcaacacgc caatggtaat atagatcgct gctttcggca gcttttccac
    10861 gatcttgaac gagccgtagc gataggcgaa ccatgcaata atggtgcaca ccacgataaa
    10921 tgccagtgca taataatagt tgttggttct ccagatcgtc tggaacgcca ttgccacaac
    10981 accgacaacc atcacaagcc cgtcgggttc gcagcggctt ttccagatgc gtttcagcag
    11041 aagccgcagc aatgtcaaaa cgacgaatcc agcaagcaca cagagcaata ccggcagcag
    11101 cggcagcttg tccacaaatt cttcccattt ctccacaggg ttcaggctgc cgccccgaat
    11161 ctctttccgg ttgatcagca ggaacgctgc cattaccatg aaatagcagc caacgatccg
    11221 catgatcaga tagggatagg gcagcttcag caccactttt tttgccgtct gcaacacctt
    11281 tttccacttc ggcggttccg gcggcacatc accttccgca ggctctgccg ccgtcgtttt
    11341 ctccggaatg gaaccggttt cttctgctgg cactgcggca tcagattctt tcgcttccgg
    11401 ctccggcggc gtatctcccg ccgcggactg gtgcagttca tcttccaaca tagttgctcc
    11461 tttctcaaga cctcatactg cggacgatgc cgcctgtatt caggaaacac cgcacaagct
    11521 ttatgcggta ttgtccttgt cttttctttc acaaaaattc tccgctatga acgatcacat
    11581 acaaccgtca aaaccaatgc ggattatttc ctattatagc attttctctg taagctgtca
    11641 atactctatg aaatttgcac tgcttttttt gtgtaaaacg acaataacta cgttatggaa
    11701 acactgaata aagcttgtgc ggtattgcct taatggtaat cagaaatgta atcttcgaca
    11761 gaaaaatctg ataggttttc tgaaatgtga caaacagcag cggcacaaag ttgtttacca
    11821 ctgcctgcac aatatagccg ataaaacagg cgtacatcgt cttttgataa gg
//
LOCUS       DAWBGR010000070        11772 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1631_length_11772_cov_5.080055, whole genome shotgun sequence.
ACCESSION   DAWBGR010000070 DAWBGR010000000
VERSION     DAWBGR010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11772)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11772)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11772
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1631_length_11772_cov_5.080055"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10758..10767
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2445 a         3399 c         2957 g         2961 t
ORIGIN      
        1 caggcatact gccgtatggc aagggatttc tatgcaatat gacggaaaat ctgcacgcag
       61 attgcgtgca aagccgtcag gcgggcttta tgcagtgttt cctttacttc cgctttttcg
      121 aaatcggcgg aacggtgtcg ataaactgct gcagctgtct ctcgaacacc tttcgaattt
      181 ccggataggc gaattcctgc tgccgccggt gctgttctgc cacaatctcc cgccgcagtt
      241 ccgcattgcg gagcacatgg tctgccgcca gtgccgcttc catgggatca ttggtgggca
      301 gcaaaatgcc gcttccgccc agcgtatcgg aaattgccga tgaatcgtat gccagaatcg
      361 gcagattgaa gtacatcgct tccaccagcg gcacacagaa gccctcgtgg tcgctcatgc
      421 acaggaacag gtctgccaga tgatagtatg ccagaatctg tgcaaaagga atgtgtcccg
      481 taaagaccac gttgtccagt tccaacgctc ccacatagcg gcgaaggcgg tggtaatacc
      541 gttccatgcc gttgtacgaa cccacgatga acaggcggga cttgggattg cagtacttct
      601 tatagcagta aaacgcccga atcacgtcct cctgcttctt gttcggagcc acacggccga
      661 cgaaaatgaa attggtgtag ccgtcgtcct gatagtgttc catgacggac tgatccggtg
      721 tcttggcgta gtcagcaaag gggatcagga tcggacgcac ggtaatgggg cagcggtagt
      781 tcagttcccg cagcacatcg ccgttatagg cggagtctgc catacagccg tccaccttgt
      841 ctgccagatg acagagtcca cgcatgccgt attctgccga ttccattgcc gcctcattat
      901 agcctttgaa gaagcggggc ggagtgatgt tgtggaatac cataagtttc cggcaggggt
      961 acttctccag agaaaagttc agttccgaac cggtggacat gtgatacaaa atcacgtcct
     1021 cgccggaaag gcggggcatt ttctccacct gctgtgcagt gccctccggc agtctgccgt
     1081 caatgacttc tgcatagatg ccggtctgat agcccatgcc gcgaatgacc ttgtccagtg
     1141 cacggacatc gtttccgatc gcatcgccca tggcaagggt aggcaaaagc tgcaatactt
     1201 tcatcgagcg tcctccccgt tcagcttcgc ctgtaatgcg gcgatctgtg cctgcatctg
     1261 ttccatggcg atcttggtgt tcttctgctg gagttccagt gcctccacct tatccagcag
     1321 ttccttggtg ctgtgcatgc gggattcccg aatgtacagt tccacctgct gctctgcctg
     1381 tgcgatattg gcattcaggc tgctctgctc tgccgcatag ggttccacat agaagctcat
     1441 gaatttccgc aggactttct tgaaaaagac tgccacggga tttcctgcca gaggacgata
     1501 cgggtctacg cggtggtgtt cgctgatata ctgcacgttg ccgcggagca tgtccgcatc
     1561 gaaccgttcc acatagatcc ctgcatctgc atctgcgggg acatcggcaa acgacagcat
     1621 gtcactgctg tatccctttt cctgaatttc tgcccgaatt ccggacatga tctcttctac
     1681 atttacgctc attgctgcga tactccttta accttccagc gaaacgccgg tgattttttc
     1741 ataaaactgc ttgtccagaa aaattgagtt gatacccgta aaggcatatt ccacataccc
     1801 cttttcctgc atgaaccgca ggatctccgg attcttcttt cccgccacca gctgtttgga
     1861 ataggggatc atttcgatga tcatggtggt gggacgatat tttgcaaagt cgatgctgcg
     1921 gaggatctcc atttccatgc cctcaatatc cagattcagg atcttcgggg caccgcccat
     1981 ctgttccatg aggtcgttga ccgccacggt ctgcatggga atggtttcca gcacccgcac
     2041 tgccggattg gcacggagca cctcagaaac atctcccgga gccgaaagcc cgtcggcact
     2101 gagcacatgg aaatccagcg tttcgccgga aaccggaccg acgcaggcgt tgatgacctg
     2161 atctttcggt cttgcccgcc gcagttcctc tgccagcttc ggattggcat cgaccagaat
     2221 gccggtggct ccctgctgat agaagaacca ggtgttgctc atatcgcagg ggtgattggc
     2281 acccaagtcc agataattgc attgtgaaag cggaacaccc agcatgacaa agatgtacat
     2341 gagaatggca tcttcgcctg cctgtgaata gctttttttg ttgaacagct ggagctcaga
     2401 cggcagcagc agacccgctt ctctcatgcc gtcctctgcc cactccatac gccgctgtaa
     2461 gtcatttgtc tgtcccgcag tctgctgtaa ctgggcatcg atgttgttgc gatgctcgtc
     2521 ctgtgcggca gcgtgcagtt ccagatgatc cagccgttcc tgcgatgccg tgttgaattg
     2581 ctgttgcttt gcgtcttgtg cttccctttc ttccctctct ttttccattt gtgccagctg
     2641 catctgctgc gttttcacct gctgttccag catctgaatg tgctgctcca gcagatacgc
     2701 accagtcgcc cgatacagac agcggcgaaa aaaccccttt ttcacaatat cgctcattct
     2761 tttttattct cctgtcttgc gttccagtgc ctttccgtcc acgaaccact gggactccag
     2821 ccggcagaca ccaatatccc gatgctctga agtcacggcg aacgcctgaa tccggcgaat
     2881 gtcatcgtaa taggtgccgt ccggtgcatg gactgccacg tccagaaaat agcgtcccgg
     2941 cagcaaatga ttgcggaacc gcacttcgat ctcgccgttg ggctgcaatg ccacccatga
     3001 gtccgattcg atctccacat tggtgccgta acagtggaca ctgtcctccc gaaagatccc
     3061 gtacccgaag ttgcccttgg gggtcttttc cggttctacg caatgatatt tgagccgcag
     3121 ggtgatctcg tcaccggtgc ggaacacata cttggattcg ccgtcctcgt ccagcagggc
     3181 ggcatctgta aactgtacct gaccggttcc cagacgaact gccgactttt cacagaatgc
     3241 cggcagcttc tgcttggaga gcttttccgc atctgcggtt ttctcctcgg caggcatttc
     3301 cgcagattcc tctgcggctt tttcggcttt ttctgcggca gcgtgttctt tctggcggtc
     3361 ggcttcttcc cgttccagac ggatctgttc catttcggac agatagtgct cgtccacgta
     3421 cttgggaacg ccttcttcct tgatcagacc gtcctcgatc cagatacacc gatcgcagat
     3481 ctgttcgatc tgtcccatag agtgggacac gatcacaatg gtagtacctt ctgccttgat
     3541 ctcctgtagc ttctggaagc actttgcctg aaagttgaca tcgcctaccc ccagaatctc
     3601 atcgatcagc agcacgtctg cgtccacgtt gatcgccacg gaaaaggcga gccgcatgta
     3661 cataccggaa gaataggtac gcaccggatt gtcgatgaat tgttccagtt cggaaaagtc
     3721 gatgatcgtt tgcagacggg aatcgatctc tttcttgctc agtccgaaaa tggaagcatt
     3781 ggtgtagatg ttttcccgtc cgctcatatc cggatgaaaa ccggcaccca gttcgatcag
     3841 gctggacacc cgacctttga tggtgatggt gccgctgtcg ggatagagga tccgtgtcag
     3901 cagcttcagc gtggtgctct tgccgcagcc gttgtgtccg atcagaccca cggcttctcc
     3961 acgctttacc gtgaaagaaa tgtcccgcag cacctgacgg ttctcatacc gccggcggtt
     4021 ccggaacagg agccgttcct taaagctctg tcccttgtcc agaaacacct tgaagtgctt
     4081 cttgacatgg tccacaataa ttgcattttc ctgtttgctc tctgccgcca ttacaattcc
     4141 tccgcaaagc cctttttcaa ttttccgaac gccagcatac ccagcaccag gaacagggct
     4201 cccagcacga ctgcctccag caggctggtc agatccggca cctgagcgta gtacagcaca
     4261 tcccgataac agctgatgac cgaggacatg gggttgatgt gatagacaaa ccgcagcttc
     4321 tccggaatca tgtcaatgga atagaagatc ggtgtcatgt acatccacac catagtgatg
     4381 atgccgagaa tgtgctccag atcgcggaaa tagaccgtga ctgccgagga cagcatcgcc
     4441 atgcccagtg ccagaaaata ttccactatc ataatcaccg gcagacacag cagagccagc
     4501 gggttcagcg gaatgccgga gatcaccatg acagggaaaa tgataaggaa gcacagcagc
     4561 atattgacaa agcagcttgt cacataggag atcggtatga cctcccgcgg gaagtagatc
     4621 ttcttgatca gatccttctg tgccacaatg gaactggagc cgccggtgat cgacgaggaa
     4681 aagaagatcc acgggatcag ggcgacaaac aggtacagat aatacttgtc gatgccggct
     4741 ttcatgatgt aggtaaacac aaaggtgtaa actaccagct gcatcagcgg gttgataaac
     4801 gtccacaaaa agcccagcac ggaaccttta taccgtccac gcaggtcttt tttcaccaga
     4861 ctgaagatca tctggcgata ctgataaatt tccttgcaaa ttttcataaa aacgtcctgt
     4921 ctggggcttg cggtactgca ctgctgtttt ctgcacggca agcccgaatc aaagtgttcg
     4981 tgcagaatac acgtatattt tattataacc gatttccgcg aaaaagtcaa tgtttttccg
     5041 gcattccgac agattgcaca agaggctgcc gctttttcga aaaatggaat cgatttctcc
     5101 aaaattcacg gcaaacaaaa agccggtgca gaaaaaaact gctccggctt ttctgtttca
     5161 catatgctgc accgcgtcag aacttacgct ctgtcttcga tgtagtttac gatgtcgcca
     5221 acggtcttga tgttttccag atcttcgtca gcaaccttga caccgaattc ttcctcaacg
     5281 gacattacca gatctaccag atccagagag tctgcaccca gatcgtcctg catggaagct
     5341 tccattgtca cttcgtcttc ttcaacaccc agctgatcta cgaccagttc cttcagcttg
     5401 tcaaaaattt cttcttttgc catgttcttt cttcctccaa taatttgatg ttgcaccctt
     5461 ctgcggaggg cggcgtcacc ggcagaaacc atgacgggtt tcgccgttca aaccggcatg
     5521 gttttagttt tcttcaaaca cgccgatttt cctaaactta gtataccgtt ctttttgcaa
     5581 aacgtcaata tctttttgta atttttctgt tatcgtttcc gccaaatatt ccttgatatt
     5641 tgccgagatt ttgtctatat cgccctgtgc ccctgcttcc ggctccggaa tgatagcttc
     5701 tgccacgcca aaacgcacca tatcctctgc tgtgatctgc atgacctcac aagcttcctg
     5761 ttcgcgggag gcatctttcc acaggatcga tgcggctccg cggggagaaa tcacagagta
     5821 caacgcgttc tccagcattg ccagagcatc gcacacgccc agtgccagag caccgccgct
     5881 gccggcttcg cccagcacca cagagatcac cggcgttttc agcatcatga actctgcgat
     5941 atttctggcg attgcctcgc cctgcccccg ttcctcggct tctacgccgc agaacgcacc
     6001 gggggtgtca atcaggcaaa tgatcgggcg gtggaatttc tccgcctgat gtgccagacg
     6061 cagagccttg cggtagccct ccggatgcgg catggcgaaa ttcgatgcct tgttttcccg
     6121 caggtttctg cccttgacct gtgccagaat cgtcaccggc aagccgttca gtctgccgat
     6181 gccgccgata atggcaccat cgtccccgaa attccggtcg ccgtgcattt catagaaatc
     6241 tgtaaagatc cgcgggatat aatcccgaac ggtgggacgg tttttgtccc gtatcaaaag
     6301 ctggcgttcc cacgccgtcc ggtgctgctg ctgttgttcc attgtcaagc acctcctgct
     6361 tcttgttccc gatcgtagcc gtggagtttc agcagatgcg acagcgtgct acgcatgctg
     6421 cggcgttcca cgatcatatc cacaaagccc ttttcctgca aaaattctgc cagctggaaa
     6481 ttgtcgggca gccgctggcg aatcgtcccc tcgatgacgc gtctgccggc gaacccgatg
     6541 agcaccttcg gctctgccag aataatatct cccagcgaag caaacgaagc ggtcacgccg
     6601 ccggtggtgg ggtctgtcat gacggtcaga tacagcagtc cggcatcgtt gtgctttgcc
     6661 actgctgccg aggtctttgc catttgcatc agagaaatga tgccctcctg cattctggca
     6721 ccgccggagg ctgtgaatgc cactaccggc aagccctttt ccgtagcggt ttcaaaggca
     6781 cgggtgatct tttcgcccac cacgctgccc atagatgcca tcatgaaatg ggagtccatg
     6841 ataatcagca cggtgcggga gcctttaatg gtgcactctc cggtgatgac agcgtctttc
     6901 agccccgtca tggtctggag ggattccacc tttctggcat accccggaaa ttgcagggga
     6961 tctgcactgt gcatctctgc atcatattcc acaaacgaac cgctgtccac cgtcagccgc
     7021 atgcgttctg cggcggtgag ccgtgcatga tagttgcagt gggggcagac cttattcagt
     7081 tcctgaaact tgctttccgg cgtggtctgc tggcagcggg gacacttgaa caccggctca
     7141 tgttcctccg gtgcgtcgtc gttaaatcgt ttggtgacgt tcttgaacag ttcttccaat
     7201 gccatgttac attctcacct tactttccgt tttccttctc gccgttcttg gaatccgcaa
     7261 ggaatttttc caaaaaggtg ttgtcatatg taccggcttc gaagtccttg ttcttgatca
     7321 gccgcagctg gaagtcaata ttggaatcga tgccgtccac gataaattcc gccagtgccc
     7381 accgcatttt catgatcgcc tgactgcggg tcggggcatg gacgatcagc tttgcgatca
     7441 tggaatcgta gtacggcgga atggtgtagc cctgatacac agcactgtcc acacggattc
     7501 ccggaccgcc ggggatatgg agcgaccgga tctttccggg cgagggacgg aagccctgct
     7561 ccggattttc cgcattgatg cggcactcga tcgcatgccc gcgaacctga atgtcctcct
     7621 gtgtaaacgg caggggcttg ccctgtgcca ccagcagctg agtctgtacc agatcaactc
     7681 cggtgaccca ttcggtgaca gggtgttcca cctgaatacg ggtgttcatt tccatgaaat
     7741 agaagttccg gtcgtcgtct accagaaatt caatggtacc ggcgttgtaa tagccgcacg
     7801 cctttgccgc acggacagcg gcttcctgca tggctttccg cagttccggt gtcatcagcg
     7861 ggctggggct ttcctccagc actttctggt tccgccgctg catgctgcaa tcacgttcgc
     7921 ccagtgccac agtgttgccc atctggtctg caatgatctg gatctccacg tgatgcggcc
     7981 ccacgatcag cttttccaga tagacttcat cgttgccgaa gaacagcttg gcttcttcct
     8041 ttgccacgga catctgttct tccagctggt cttcccgatc gatccggcga ataccgcgtc
     8101 cgccgccgcc ggcagaggct ttcaccagta ccggatagcc gatctctgcg gcgatcttct
     8161 tggcttcttc cacagaagaa atggcaccgt ccgaaccggg tattaccggc acgccggcag
     8221 ccttcatggt ttccttggca cgtgccttgt cgcccagcat ggagatcgac tccggtgacg
     8281 ggccgatgaa agtgatgccg ttatcgatgc aaagctgtgc aaactccgca ctctccgaca
     8341 aaaagccgaa gccggggtgc agtgctgttg caccggtatt ctggcacgcc gagatgaggg
     8401 cgttcatgtt cagataactg tcctgtaccg agggcggtcc gatgcagacg gattcgtccg
     8461 cgatctctgc atgcagagcc gtccggtccg cagtggaata gaccgtgacg cactgcaagc
     8521 ctgcttcacg gcaggcacga atgatacgca cggcaatttc accgcggttt gcaattaaaa
     8581 cctttttcaa catcttgctg ccgccttact gctccactgc gaacttgatt tcgcaggtca
     8641 cggcacgctt gccgtctacg ctggcgatcg ccttgccgac acctaccggt ccacgctgtg
     8701 caatgatctc cacttccaga tccagcacat caccgggaac tacctgccgg cggaactttg
     8761 ccttatcgat tccggcaaag agggcgatct tgcccttgaa ttccggctgg gacagaatgc
     8821 agaccgcacc tgcctgtgcc agcatctcga ccatcagcac gcccggtgtc accggctttt
     8881 cggggaaatg tcccttgaac caaaagtcgt ctgccttgat atatttccgt gccacaacac
     8941 gctttcccac ttccatttcc agaacctcat caatgaggag gaacggggca cggtggggaa
     9001 tgatctcctt gatctgttcc tgattgagca atacttctgc catgtctgca ctcctttacg
     9061 aaagaatcat cagcggctgg tcatattcta ccgcatcgcc atttttcacc agaatttcct
     9121 gtactacgcc gtccacgtcg ctggtaactt cgttcatcag cttcatggat tcaataatca
     9181 gcaccacgtc gccctttttc acggtatcac ccacctgtac aaacggcggc tgatccggag
     9241 agggagccgc atagaaagtg cctaccagcg gagccttgat cgacttgcct gcgggcttct
     9301 gggaaactgc cggtgtttct gccggagctg ccggagcagc agcggaaact gccggcgaag
     9361 cagccacagg tacacctgcc agcggcatcg gcggcaggca cttcttaccc tttacggtaa
     9421 tatatttttc accgtccgca atggtgatct cgcccagttc ctttgcagcc acgatgtctg
     9481 ccagtttttc aatggtttcc agatccacca tgttataaat tttttcactc atgtctgatt
     9541 ctccttagtc tgtgcacttc ttgaagcaga gtgtcgcatt gtgtccgccg aagcccagcg
     9601 agttggacag tgcagcattc accggcatct ctaccggacc gtccacacag taatccagat
     9661 cgcactccgg atccggtgtg gtatagccga tggtctggtg aatgaagctg tcccgaatgg
     9721 acagggcaca aactgccgct tccactgcac cggtaccgcc cagcatgtga ccggtcatgg
     9781 acttggtgga gttgatcttc acctttcgtg cctgctcctc gcccagagcg atcttcaccg
     9841 ccttggtttc cagcatatcg ttcaggtgtg tggaggtgcc gtgggcattg atataggtga
     9901 tgtcctccgg cttcagacca gcttcggtat aagcctcgat cattgccttg gaagatgcac
     9961 cgcattcgtc tgccggagct gtcatgtggt atgcgtcgca ggtcgcaccg tagccagcca
    10021 gttcgcagta gatctttgca ccgcgagcct tggcgtgttc gtattcctcc agcaccagta
    10081 ccgcagagcc ctcacccagt acaaagccgc cgcggttcag gtcgaacggg gtagaagcct
    10141 ttgccggatc gctttcggta gtcagtgcct tcatggtgcg gaagccgccc agtgtgaatt
    10201 cggtaatggt ggattcgctg ccgccgcaga ggcaaacgtc cagatagccg tccttgatct
    10261 tgcggaacgc ttcgccaatg gcgtgagtcg ccgatgcaca ggcagtggtg gtgcagaagt
    10321 tgtcgccgcg gaagccggtg cggatcgcta cctgtccggc agccatgttg ccgatcatca
    10381 tgggcacaaa gaacggagag atccgtcccg gtccctttgc acgatacttg tcgtgttctt
    10441 cttcgattct gccgatgccg ccgatgccgc agccgataat ggtgcctaca cgatacggat
    10501 ccatatcctt gaagtccgtg cctgcgtcct gcaacgcctg aatcgcagat gccacggcga
    10561 tctgggtaaa gcgttccatg tggcgaatgt ccttgcgttc aaaatattct gcggggtcga
    10621 aatcgcggat aatgccggca acgctgacac ccagttcggt ggtgtcaaag ctgtcgatct
    10681 tggaaacacc cagtgttccc ttttttacgt tttcccagaa cttttctacg gtgctgccca
    10741 gcgggtttac cgtacccnnn nnnnnnnccg tacccagtcc ggtgattact actcttttca
    10801 tatttcctcg ctctctgccg ccgtgctgcg gcaagcttgt ttttcatctc aaagcattct
    10861 cagactgcct tccctgaaag agaaggcgaa cctgcgaagc tggtggaaaa gtcccttaca
    10921 tcatcagtcc gccggagatc tcgatggtct gaccggtgac atagcttgca tcttccgagc
    10981 agaggaacga gatcacgctc gccacttctt ccggattgcc gtaccgcttc attgccacca
    11041 gttccagcat cttgttccgc tgttcctcgg tgagagcgtc agtcatgggc gtcttgatga
    11101 agcccggagc aacggcgttg acggtgacat ttctggcacc cagttccttt gccacggtct
    11161 tggtcatgcc gataatggca gccttggaag cggaatagtt gatctgtccc ggattgccgt
    11221 acagaccggc taccgatgcc agactgacaa tgtgtccgtg ccgctgccgc atcatgacag
    11281 cacccaccag cttcatcatg ttgaacacgc ttttctggtt gacggcgatc acggcatcat
    11341 actgttcctc ggacatacga accatcagac cgtccttggt gatgccggcg ttgttgacca
    11401 gtgcgtcgat cgagccgaaa cgctctttta ctgccttggt cattgcttcg cactgggcat
    11461 aatctgccac gtttgctgcg aacagttctg cctctacgcc gaagctgcgg cattcttctg
    11521 ccaccttttc tgcatttgct ttatcggaat cgctggggaa atggttgatc gctacattgt
    11581 agccgtcctt tgccagacgc tttgcgatgc aggcaccaat gccctgcgat gcacctgtga
    11641 tcaatgctgt tgccatagtg atttttctcc tttatttagt atctatctgt atctgcctta
    11701 gttcttgcct agggaatcac tgaataaagc ccgcctgacg gctttgcacg caatctgctt
    11761 gcagattttc cg
//
LOCUS       DAWBGR010000071        11656 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1648_length_11656_cov_3.807689, whole genome shotgun sequence.
ACCESSION   DAWBGR010000071 DAWBGR010000000
VERSION     DAWBGR010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11656)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11656
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1648_length_11656_cov_3.807689"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8705..8714
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2565 a         3510 c         2853 g         2718 t
ORIGIN      
        1 gctttgcata gattttcttt tgaatctttg tctaactttt tggggtcagt tcaaaagctg
       61 atgttccgct ttattttgca atattttctg tttacgcacg gattttcgag cacaggctcc
      121 tatacaaact gattgcgttc cggcttgtat acgaagccaa ctgcttccag ctttgcggtg
      181 atctctgcct gttccagaca catagacttg cacagttcct ccagagaggc atagttgtcc
      241 cgcagctggg tattcagata actcatcagc atgatcggat cgttaggaat gttcggcatt
      301 accgttcacc tccctctggt gacaattcca gcatatgctg tacatgtgcc acatagagtg
      361 cctgcaccgc cggatatgct cccagcccct gcatttgcag tgtgacagta tagccgtgtc
      421 tggtcaatac agacttccac gaatcttctt cgtcccctgc catatcgttc tgggcgtggt
      481 cgcctgccac cagcatcatg ggcaccagca ccacttcctg atactgccgt tccttcagct
      541 gttccaaaag ggtatccagc gtgggatagc cctctaccgt tcccacaaaa acattgtcga
      601 acccgcaccg tttcagccag tagtcaaatg ccggatacgc ctgattgatg atgtgcggcg
      661 tgccgtgtcc cattaacaga tatgccttgg tcgatgcctt ttctattacg ccgtcctgct
      721 ccattgcctg aatcagtgcc ggatagtctt ccgtttctgt cagcagcggt ctgccgatgc
      781 ggagcaccgc aaaggctcct gcatacgatg ccgcatctgc acaaaggcgg tcgtactcct
      841 cgccgggaat gacatgagtc ggctgaatat acacatgggt ataccctttc tgtttcagct
      901 tctccagtgc ctcccgcacc gtgtctaccg cagtgccctg ccgccgcatc ttccgaatga
      961 tcatgccgct ggtaaatgcc cgaaaaacgt ccatttccgg aaacgcctgc tttacagcag
     1021 attccactgc accaatggtc tgctctctgg tgtccgcata gctggtgcca aagctgacca
     1081 tgagcagtgc gttccggcat gccggcagct gttcttctgt caggctggaa gcatatgcca
     1141 gatatgcccc ttttcccttt tcccgaaaca gctccaacgc tttcggctcc gtcagaattc
     1201 gcagcagctg catgcggcga atttcgtccg gcatggtttc tttccatgcc cgccgcagga
     1261 ttcccagaca ggtgcaaagt tcgctgtact ctgccagctt cggttccagt tcccgacaga
     1321 tcgcccccgc cagtgacgga gcatcgccct cggtcgtcac tgccatggta atgtcgccgt
     1381 gtttccgatg tgccggaaca gtaaagtccg gcgtgcagtc cggcgataca gttgccgacg
     1441 atgccagctt tccggcagcc tgagcttccc gtacaatgcg ggcattttct gcactgtcat
     1501 cagttgcagc aaacaccagc aggttttccc ccagcagttc cggtgcatag ggctgctgca
     1561 aataagtcag ctttgcgttt ttccagcacg ccggcatagg ctccggtgct gccacggtga
     1621 cttccgcacc agattccagc aagccccaga cacgccgctg tgccactttt cccgcaccga
     1681 acacgatgca ctttttcccc tgtaaatgca atgcgatagg atacatattc tacacctcgt
     1741 tttctttccg aatgcatgcg atcatgccgg ctatatccga ctgtaccgca gtttccgcca
     1801 cagaaagtcc tgccgtttcc gctgcctttg tggtagcatt tccgatagag atcagcttcg
     1861 ccgcagtttc tgttcccgcc gtcatttctg caaaggcacg tgcagcggag gcactggcaa
     1921 ggaaaacata gtccgtatct gccagttcct gcaacagcag ttcccgtttc cgcaggtcgg
     1981 tctgcacccg atatagcggc actgtatcga cggctttccc cgcctgttcc agcagctgcg
     2041 gcagaatgac agagccgttt tccgctcgca gcaaaagcac acggtcttgc atggtcagct
     2101 gcgggatcag tgcctctgcc agactgcggc tgtcgaagtg ctccggcaca cagtccacga
     2161 aaatgccgta ttccgccagt gcctgtgccg tgccgtttcc aatggcagca aaatgcagat
     2221 gcagcagccg ccggaggtct acatgggatt gcttcagcga tgcaaacagt tgttccacgc
     2281 cgttggcact ggtgagcact gcccacgtat acgactgcca gggcaacgcc ttcagcacgt
     2341 ccggcgaaga agccttcggg tagatcaggc tgacttccac cggctctgct ccacaggaac
     2401 aaagctgttc tgccgccttg cgggtatacg ccggtgtacc ggtcagcagt acctttttgc
     2461 cggacagttc gcccattcca aaccacgaca gctgttgccg cagagaaacc acgtcaccga
     2521 tcacagtcat tgccggcgtg tggattccct gccgctgtac ttctgccgcg atctcagaaa
     2581 gtggagccgt caccacacgc tgccgtgcgg ttgttccctg ctggatcact gccgccggtg
     2641 tatccggaga tttcccctgc tgtatcagcg aagcggcaat tgccggcagg ttgctcaggc
     2701 tcatcaggaa caccagagtg ccgtccagtt ttgccagcgt ggcatagtcc agttcggcat
     2761 gctgttccgt tttgcggtgt cccgtgataa catgaaatgc aggagcgtat tcccgatgtg
     2821 tcacagggat tccggcataa gccggcacac tatagcagga ggaaacgccg gatacgatct
     2881 gaaaagggat tcccagcgat tgcagcacct gtgcttcttc tcccccgcgt ccaaagacaa
     2941 aggggtcacc gcccttgagc cgtaccaccc gttttccctg ctgtgccaac tccgccagca
     3001 gttgattggt ttcactctgc tttttgtggt gatttcccgc cgtctttccc gcgaagatca
     3061 gttcgcagtc cggtcgtgtc aggttcagca ccgacgaaca cgccagtgca tcatagacca
     3121 ccacgtccgc ctgttccaga caagcctttc cctgttctgt cagcagtgcc ggatctcccg
     3181 gacctgctcc caccagaaat accgttcctg ttttcatggg tattcctcct cactccgccg
     3241 catgccgcag caattctttt cttgcaccgg aaatggtaga aaccgaagca tctacgtccc
     3301 agtgatcctc cttgtgcaaa atttccagca aatgcgaaat tcgcggcaga cgcagatgat
     3361 gctcccgcag cagttccggc tgggcgaaaa tttccgccgg cgtaccatgt gccaccatgt
     3421 gtcccttgtc cataacgtcg atcacatcgg cgtaaatggg taccacatca atatcatggg
     3481 tagataaaat aatggtggtg tgctgctgcc ggcagacatc ttggagcagg tgcatcagtt
     3541 cactgatgcc cacagggtcc agtcctgccg tgggctcgtc cagaataatg acttccggct
     3601 gcatgaccat aatgccggca aatgccaccc gctttttctg tccgaagctc agggcgtgag
     3661 tgggacggtc tttcaggtat gtgattccca cccgttccat gaccgtttcc acacggcggt
     3721 gcacctcctc cgcgggcaag cccatgttga ctgctccaaa agaaatgtcc tgatataccg
     3781 aagccgaaaa cagctggtcg tccggattct gaaagacgat acccaccttg gaacgcagtt
     3841 gccgcaagcc tgcttttttg taggaaatcg gctgcccgcg aaagcgaacc tctccctgct
     3901 ccggctgcaa aatgccgttg agatgcagaa acagcgtaga ctttccggca ccgtttgctc
     3961 ccagaatcgc ggtagtcttg ccttcttcaa atgcaatgga caaatcctca atggcgtgac
     4021 tgctttcgtc atagccatag gtgagatgac ttgcctctaa aataatcccc atacactgcc
     4081 tcctattctg caaaagagca cggctccggt caggagcaca ctgtatccga cggcgagccc
     4141 tttctgccag cctgttgttt catatgtcaa tcccagtgtc cgcacttcat cggtgtaccc
     4201 tctggactcc attgccgtat aggtacggtc tgcctgccgg tatgcccgca ggaacagatt
     4261 ggagatcagc tgtcccatgg agtaaaagct ggtctttaac gagccatacc ccaggcggca
     4321 ttcctgtgcg ttccgaatct gcgaagccga ctccatcagc acaaagatat accgatagac
     4381 cagttccatc atctccacca gaaacgccgg cagatgcaga cggcgtagtg ccgtaaacag
     4441 ttctgtcatc ggcgtagtgg cagagagaaa atacagacag ctgactgccg ccagacactg
     4501 taaaaacacc tgtccgaact gatgcagact ctctccggtg atgccgaagt accagcttcc
     4561 cagatgcagc gataccagca tggactcgcc atgatgtgag atctgcagca ggatcatgat
     4621 gcaccccagc acaacaaaag ccagcgggat ctgcattaag tggataacat gatgccaccg
     4681 ggttttcccg atggcagtca tcgcggtcag catgcagcat aagatcagca cgctcaccgc
     4741 ccatgtatgt gagatcaggc acagaagcag actatacagg gacacaaaaa ttttccagaa
     4801 cggagaaaca cgccgcaatg cagatgcata ggcgatctca tcaatgagga ttcgttctcc
     4861 gtgactatgg gaatgtgtat gtcgtttcat ctgttgcctc ctgcggtcag gaagcagaag
     4921 tttgcttctg gtctgtcaat gcagcatcgt cctctgatgc agatgctttc ggtttccggc
     4981 tgatgcgtcc cagcacaaag ccgcagatgc ctgcaccgat cgccgactgt acacagaaca
     5041 gcagagattc gacctctccc gaaggcggtt cccagaagga ctcaaaccac ggggtgtagc
     5101 cgccgttgat ttcttctact acagtagaac cggcatcatc agaaccgccg aattcactgt
     5161 tgcccagcag catcagcgga atggtgacca gtaaaacaac acccagtgcc agcaccagat
     5221 ttcttgccca caatttgccg cggttggggt tggaaactgt aatgccggac agttccttca
     5281 actccttgac gcagttcttc tgaatgacat tatagacaat gacagtcaga atgccttctg
     5341 caatggcaat ggggacttga gtcggtgcaa aaacggcaat gaacttgccg aacgatgccc
     5401 agaagttatc tccgggataa gcagctgcaa gctgtaccga agtcaggcag taagttgcca
     5461 tatctccgat cgttgccgac agaaatactg ccagtcccat cggtgccttg cactttttca
     5521 gcagctggaa cagtccgtaa gaaacgaacg gtccgaaaat cgccatggaa aatgtgttgg
     5581 cacccagtgt ggtcagaccg ccgtgtgcca gcaggatcgc ctgaaacagc agcacgatga
     5641 tgcccagcac ggacatgatc gacggtccga acagaatcgc acccagtccg gtaccggtcg
     5701 catgagaaca gcttccggta accgacggga tcttcaggga agaaatgata aagacgaagg
     5761 ctgcacaaag tgcgatcagc agcttcactt ttccctgata cgcgattttc cgcttcaggc
     5821 tgactgcacc agccaccaga aacggaatgc aaagtgcacc ccatgcaata caccagccag
     5881 cgggcagata gccctccatg atgtgcatcg cagatgcacc gaatttgccg cttgctgcca
     5941 tatcggacag cagcagacaa actgccagag cagacaccag cacacgacat cccgtcatgc
     6001 ctctggtgct gcggttctgc aaaacagagc cattgtgttt gttcatatga aaacctctcc
     6061 tttcggaacc cctctccgtt ggctcccggc tgagaacaga aagaggttct cagtttccgc
     6121 tgtgtgcaga gaaaaatgca tctgcatcac ccagcgtttt atctatctca cgcagcgtgt
     6181 gtgcgttcga tacaaaaatt gcttcaaact ggctgggacc gaaatagttg ccctcatcca
     6241 gcatgaaatg aaaatattcc cgaaatcttg cggtatccgc ctttttggca tcagtatagt
     6301 tccgcaccgg ttccggtgta aaatacaggc aggacagcga accgataccg ttgacggtac
     6361 agttgccatg gtgccggcga ataatgtctt ccagaccgct gcgaagccgg tgggacagga
     6421 agcctacctg atgataaact tccggatgtg cctgcaaata ccgcagctgt gtcagaccgg
     6481 cacgcattgc caccggattg ccgctaagcg tacccgcctg atagacagag cccagcggag
     6541 ccaccagctg catgatctcc cgtctgccgc cgtatgcacc gaccggcatg ccgccgccga
     6601 tgatcttgcc ataggtcacc agatccggtg tgacaccaaa gtgtccgaca gcaccgtcca
     6661 gctgcagacg aaatccggta atgacttcgt ccagaatcag cagagcaccg tactggtcac
     6721 agatctgacg gattccctgc aggaatccct tttccggaac cacaacgccc atattggcag
     6781 ccaccggttc cacgatcacg caggcgattt cctccggata ctgctgaaac agctgttcca
     6841 cgctgtccag atcattatag actgcggaca gagtatccgc ggtacagcct ttcggcacac
     6901 cgctggaatc cggaatgccg gcagtcatca cgccagagcc ggctttcacc agcatagcat
     6961 ctgcatgccc gtgatagcag ccctcaaact tgacgatctt gtcccgtccg gtaaatcctc
     7021 ttgcggcacg gacagcactc attactgcct ctgtgccgga gtttaccata cgcatcattt
     7081 ctacgcaggg aacgatctga tgaatcagtt ctgccatttc cacttccagt gccgtggcag
     7141 caccgtaact caagccgttt tccagctgat tccggacagc ttccagcaca atgtccggat
     7201 tgtgtcccag aatcatcggt ccccaggagc caatataatc gatatagacg ttgccgtcct
     7261 catcgtagag gtactggtct ttggcacgct gaataaaccg cggcgtaccg cccacagact
     7321 gaaacgcccg caccggcgaa ttgacaccgc cgggcatcag ttccttggca cggtcgtaca
     7381 acgcctcgga atgagcaatg tgcatctgtt ccattatccg atcctcccct cgcgaatgta
     7441 cttcgccagt tccattgcaa agtatgtgat caaaagatct gcaccggcac ggtacatgca
     7501 gaccgctgat tcgcaaatga cagcttcccg atcaatatag cctgccgcag ccgctgcctg
     7561 aatcatggca tattcgccgc tgacgctgta cagtgccacc ggctggcgga attgttcttt
     7621 cagcgatttc agcacgtcca gatacgccat acccggcttt accatgagaa tgtccgcacc
     7681 ttcctgcacg tccaattccg cttccagcaa agcttctctg gcattgtggg gatccatctg
     7741 atagcttttc cggtcgccga acgccggtgc agaaccggct gcgtcccgaa acggaccgta
     7801 gaacgccgaa gcatacttta cggagtacgc catgatcggc gtgttgacaa agccgttttc
     7861 gtccagcacc tgccggatac gtcccactct gccgtccatc atatccgagg gggcaaccat
     7921 atccgcacct gcctgcacat gggacagtgc tgtttttgcc agcacttcca gtgtcttgtc
     7981 gttgtccacg tcatgcccgc agagaatgcc gcagtgtccg tgcgacgtat actcacacat
     8041 gcagacatca gtaatgacat acagctgcgg gaaccggctc ttgatatgcc gcaccgcctg
     8101 ctggacaatg ccgtccggct gccatgcact gctgccgatc tcatcttttt gagcagggat
     8161 cccgaaaagc agcacgcttt tcacaccgga atccacgatc tgctgcaaga gttcatctac
     8221 gcagtccaga ctccagcgat actgtccggg catggaggga atctcttccc gaatatgctc
     8281 cccctcctgc acgaacatgg gatagatcag tgtatccgca gaaatacggg tttcccgcac
     8341 catgctgcga agcagagcac cgtttcgcag gcgacgcggc cgattggtca gattcatatt
     8401 gataccttct tacttattcc gtaatggtca gttttgatgc cagctgctgt accagcaagt
     8461 ccgcatcatt tcggtttccc tgcacgctgc cgtaccgcgg caccccgccg ataccggaaa
     8521 acccataaag ggtcatgtcc gttccatgca gctgtgcatg acagcccacc ggtgccgagc
     8581 agtccccgcc cagcagcgat gccagctgcc gttctgtttt cgcacaaagt gccccgtcgg
     8641 gggaattcag ccgggcaagc aggtcggcat attcgccggc acgagtctgt atcgccagaa
     8701 tcccnnnnnn nnnnccggca ccagttcctc cggtgaaaag cggtaggcga tccggtcaga
     8761 cagccccaac cgtttcagcc ctgcctccgc cagaatcagt gcaccatact caccgctgtc
     8821 cagtttttcc agccgtgtca gcacattgcc acgcaccggc tgtatcactg cttccggata
     8881 cagctgttga aactgcatct ttcttcgggc actggcacac cctaccggca gtgccggatt
     8941 ccagtgtgaa gtttccttcg ggaacaccag cacatctgcc ggcagttccc gcacagagta
     9001 tgccccaatg ggcagtgccg gattctctgt caccggcaca tctttcaggc tgtgcacgca
     9061 gatgtccacc tgtccggcga gaagtgccgc gtccagttct ttcaaaaaca gcccttttcc
     9121 gccgatcttg tccagcgtct tgtgcaggat cttgtcccca gtggttttca tggtcaccag
     9181 ttccaagtct gcttccggca ggatcttccg gatctctgcc atgaccagtt cgctctgtac
     9241 cacggcaagc cggctggcac ggctgccgat acgaattttt cttcccatgg gttcaaaatc
     9301 ttcctctgtc tttgattttc gcacagcttt gccgcaccag ttccggtgta atttccgatt
     9361 tcatgctgcc catgagcata tccaccgttc gttccgtcac cagccgcacg atctcctcca
     9421 gctgaggcag ttctgcataa ggttctgctg ccgtactgtg caggattcgc tgtgttgtca
     9481 gctctttcag ctgctgcata tagggcaccg cgtcccgata attccgccac atctgaaagg
     9541 tgtcggtata ctgttccgcg atttgccgca gagccgccaa cgccgtttcg tccggagccg
     9601 tgctgctgtg caggtcgtcc agattccaga gcgtaacacc ttccactgtg ccgcagtctg
     9661 cttccacgtc ccgtggcatt gccagatcca gaatccagac cggctttcgc gagagtgctt
     9721 ccagctgctt tttggtaatg gtgtgatgcg gacttctggt ggcactgatg acgatctctg
     9781 ccgcttccat ttgcaggaaa cgctgttcgt aaggaaccgg ctgcacaccg aacgggatca
     9841 ccgtttcccc gtgatgatag gaacgcagtg tcataaacac acgacagccg tgctgcacca
     9901 gcagagaagc tgccagccgt cccatgctgc cgttgccgat caccagacag gttttcccgt
     9961 ccagtgttcc ggcatgctgt tccagctgct gtaccgcact gtaagaaacc gaaagcggta
    10021 cattctgcac ggacacttgt gtctgtgcct cttttccggc agaaactgcc gttcggaaca
    10081 gggtgtccag cacgccgtct gtactgcaac aggcacgtgc cagttccacc gcatgtccca
    10141 cttgtgtgac gatctgttct tcatgcagga tctgcgaatg cagaccgcag gcgacttcca
    10201 tgatatgctg taccgcatcg atttccgtca aaacccgccc tgtaaaagac ttttccggtg
    10261 catgggcata ctgccgcagg agttctgccg gagagatgtc cattgccggc tggctgacag
    10321 catcgtcttc tgcggaagac cggcgttcct gtacggagag atacagttcc gtccgattgc
    10381 aggttgcaag caatactgct ccgctgatgc cgtctttctg caccagttct gtcagaatct
    10441 gcctgacctg ttcttccgaa aatgcaaact gttcccttgt ggaaacgtct gcggaatgca
    10501 cgtccactcc tgccatataa atgcacatct cgttgtttca actccctgat gcagcaagcc
    10561 gcagggggga taaagtcacc accctgcaaa aaaccggcgt acaaggtttt acctgccatg
    10621 aaaaatacgc tttctgctgt tgttccaatg cagaaaacac cgccataatt gtgcctccgt
    10681 gtactactgc tgcgatatga gattcctgcc ggctttgcag tgccgtttcc aacgctgcac
    10741 agcatcgccg cacaaattcc tctctggatt cgccctccgg aaatgccagc gtaccgccgc
    10801 tgtcgatcca cgcctgatag gacgtgtcac cggacaactc ctgataattt tttccttcga
    10861 atgctccgaa atcgcactcc tgtagtcccc atacgatttt ctgcggcata tggggaaaca
    10921 ggatctctgc cgtctgccgg caacgctgca tcgggctgac cagcaccagt tccggtgccg
    10981 gaaacgaccg gcgaaattcc accgatttct gctgtgccag agcgattccc gcttcgcaaa
    11041 gcggttcgtc tgttcggcag ccgatatagc ggtgttccag attgccggct gtgatgctgt
    11101 gccgaatcaa gagcagctgc ataagtttcc tccaatgtca aaaaagcccg tgtttccaga
    11161 agaacacggg atacaacaca caggccttgt gtgattgtct tgggaaatac cgatcatatt
    11221 tccttcgtat gggtgcaaaa agcagagcct ttgcagttat gcaaagcaca gaatacacat
    11281 gcaccaacaa tttcctctat tctcgtagat gggatctatt ccaaagcagc agaccggtat
    11341 cccgacttcg gctctccgcg gaccatacgc cttcccggaa cggaacattc cagtggcatg
    11401 tgtatggtgc tccccgacac ggtagcggaa ctgtgcagga ttctcacctg cttccctgac
    11461 atctgctgcc aattgttcga taaaaagggc ttttcaaatg cccacgtact actataccac
    11521 atttttcgca gattggcaag tctttttgca aaaaaagcag tcctgtacgc atatcataca
    11581 ggactgcctt tactctgctc ctaaggaaac gctgaataaa gcccgcctga cggctttgta
    11641 cgcaatctgc ttgcag
//
LOCUS       DAWBGR010000072        11234 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1698_length_11234_cov_3.205117, whole genome shotgun sequence.
ACCESSION   DAWBGR010000072 DAWBGR010000000
VERSION     DAWBGR010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11234)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11234
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1698_length_11234_cov_3.205117"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2626 a         2539 c         2646 g         3423 t
ORIGIN      
        1 ggcctgctgt acggcagcct gttttgcttt ttcgtccttt tcgtctgcat cgatcagaat
       61 cgaaccaatg tcgctgtcga tggcgttttt caccagattg tgatccggaa aatcattgcc
      121 catcaggtca ttgatgtagg cgttcatttt ggatttgttt ttcaggattt cctttccgcc
      181 catttccaga cagtaggcaa ttccctccga aaccgaatcg aaatactgga acttagagat
      241 aaatttttct ttgcaatggg gacaatgctg cttatctgac aatttttcgt caaacaagct
      301 ccatgtctta ttgcatttgc tgcacttcat aatcagattc ctcttttctc acatactgcc
      361 gccttttagc gacatgttgt tttgtgcttt tgtttccccg gataatccgc accaggcaca
      421 cgttgcatag gattccccat ggtagcaggt aatcttggaa cagcgtggac agtgtaaaaa
      481 agttttggtt ccgcaaaagg gacagccgtt ataattgggg gacgggtgaa acgttccgtt
      541 caaaacagtt tcgtcgtaac cctccgattt tgctttgctc tcgtcaatcg ggaacgccca
      601 tgtcagtgcc cagtggtctg gctgcttttc gactcggatg ccgtataagt gccggttgca
      661 ctgggggcag cgtgcaagaa caatgtgtgc ctctttgatc gccatgtttc tcactcctct
      721 tcccattcat tttcgctttc gctgatgatc tcttccggaa ctgcgtcatt tacttcgccg
      781 gcattgtcgc tttctgccga atccggttct gctgcatcca gagagaattc ctctggctgt
      841 gcggatgcgg tttctgtttc acaggcggga atgtgggctt cctgtgcatc ttgctgcgct
      901 gtgatttcct gtgaccggtc atcctctgaa accgtgggac agattgcagc gttgtcttgt
      961 gttttctcct ctgtatcagc agcttgcacc tgaccagaag catcgtcttt ttcttcccag
     1021 cctctttgca ctgctgcaat atcccgtgtg acacctgcca gcttctgctg ctgttcagag
     1081 agcttttcct cgattttacg tttcttttcc agataccgct tgattttttc ctcggtttcc
     1141 ggcgtttcag ccgattgtga caaaaggcgc tgtatctttt ccagctggaa ctgcaattcc
     1201 tgaatggtgt cctgcaaatg ctttttctgt gcagttagcg tctccaattt tgtgatctgt
     1261 tgtaaggttt gttcgatagc ggataacttc tgtacatcca gaatccccgc aatggaaaca
     1321 tcgtcatgac tgccggattt ggagatgttt gcaagattgg cttgcagcat ttctgttcgc
     1381 tttcggaaat tgtcgtgtaa aaaatcaagt gcaacgttgc ggtaaaaaga gtgcagcatc
     1441 tcggcaaatg tatcatcgat accgtccgtt cctagaaaaa tggcaacggg aatctggttg
     1501 tcccagatgt ggcagcgaaa ttcactggct gcatttttat cgcaaatgga agtgcagcgg
     1561 ttcaaatggc agttctcatc ccatggaatc ggctcgtcca tggtcccgtc ctcgtatagg
     1621 gcaacgcatt tcccatcgcc gatgtgcaga ccgatggcgt aggatttgca taacccgacc
     1681 gcaattagag tagtaccgta ggctttctgt acgtgagaat gctccttttg tatcaggtct
     1741 gagacctcgc tttccgtatg tgctctgtag ttttcctcgg tgaaaaattg aaaggttttc
     1801 tggtactttt ccggcactct ctccagttcg ttcggcgtga aaggggattt gatcaggtca
     1861 tttctcacgg caatatgcca ttccgtaatg atgctgtgga tcagctgtgt caccacactt
     1921 tttttcgttt tggagtctgg aaacggaaag gtgattgatt ttagaaattc ccgaatctta
     1981 ttgcaagcga tctctgcggc aaagcgggaa cctcggtcgc tccggaagta ctgcttgctg
     2041 ccgtgaccgt ctgccacagt tgcaacgaca cagtccgcat cctgcagata ttgtgtactg
     2101 tcttggcata cggtatggtt caagatatgc tgatagccct tttctgaaac ggcaaatcct
     2161 tcgtacatca tcattgttta atcccattcg tcatcatcat cggaatcatc cgaacccagt
     2221 atcatcggtt cgggcgattc ttcggtgctt tctatactgc tgtatgcaga ttccttttct
     2281 tcttccagca taacctttgc ggcttccgcc atctgatgtt ccgcagacgt agaacggacg
     2341 tccttcacat ctgccggtgc ttccgcagaa tcctcggaaa ccgttgcaga ggtgcttgcg
     2401 atctgtgagg aggtgactgc aatgaaacgg atgatctttt tgaggcttcc aatgtcattt
     2461 acggtaaaca gaagttcaac attgcgaatt gcttttttca gcacttcccg gttggcatct
     2521 tcgccgattg caattgcaat tttcaacgca gcctgatacc acttattttg ctgtagcttt
     2581 ttcagtgcag tgaggtagtc atctgttggc tcgccgtcag agaggagcag gatgacaggc
     2641 gcataaaatc cggaagatgc tgcgcctcgg tccatgaatc catttttgga cagcttgtcg
     2701 ttcagctcca gaaatgcagc tcccagatcg gtcaggccgt tggcgtccag cggttcccaa
     2761 tcatcaaagg cgtccagaga catcagagca ggcgtgaccc attcacagcc ggtggaaaat
     2821 ttcaacacgc tcagtttgat ttcggaatct gcctgcgatt cggaaagatg ccgcagatcc
     2881 ggaatcagtt cctcgattgc agaattgacg gcaccgattt ttttgccctc catgcttccg
     2941 gaggtatcga tcaggaaaaa gatcggcatg actcttttcg ggatgctctc tgcatttccc
     3001 agaaaactgc ttagattttc agacatagtc tagtctcctc ttctgttatt tgatttccat
     3061 atactcattt ttgatttgaa tgcggattcc cttttgaatc agaacacgct ctccgggagc
     3121 gacagtttca ttgttgatcc gccaagattc gccggacttg ttcatgagtg caattttggt
     3181 tttggtttta ttctgtacga cctttgctgt aactgtccgg cattcgtttt ggtcgtagca
     3241 atctgtgatg taggctgtaa caatctcctg cttggggaaa atcggaatgc ggaaatattg
     3301 tgtctggatc cagccaatgt gcgtgattgg ctgatgacag cacatacacg ttgcattttc
     3361 agtggtatat ctggtttctt ttccgcaatt gggacagatg atgacttccg actggaaccg
     3421 aagcagattt tgcagccact ccttttccgt gattcccatt ttgtcttcgc acatgacttc
     3481 cttgctgaaa gccttgataa aggtattctg aatatatacc ggcatctgcg gccatctcat
     3541 caggaagtta ttgtgcacgc cctttaccgg acggttggac gcatccttgg ggtcggcaat
     3601 gaaaacaggg gattctccgt aatagcgctt ctgtcgttgt tccgtcatga cggccttgct
     3661 ctgcgtctgc tcgccctcca gagggtggtt tctcagcaga agcaggaaaa ggacaacggc
     3721 aagagaaaac tggtcggttc tcttatccgg cgtctttctt cctacaacga tagatggtgc
     3781 catgtatctg cattttccag caatcccgga cttttctcca aactgggaaa cgttgtcgtt
     3841 atcgcagatc aggacatctc cggtttcagg gttcaggaag aagttgccgt cattcaaatc
     3901 ctgataggag aatccacggt tatgcagaat ccggaaactc tctacaatgt tgatggctgc
     3961 attcagcagc gtgtgaacac tggaaaagtc aatctttccg atcaggaaat cagaaaagtc
     4021 atagtagtta tcggggcgca gtttcataat gtagccaaat gtatcgtagt acatacgggt
     4081 gatggcaacg ggccacagga atgagtctgt gggagcctgt tcccggatgt tgttttgcag
     4141 gttcttgcga aatccctcca tgtcctttat cgaacctttt ttgtaccatt tcaatgccat
     4201 ctggtttccg agatagtcca ccagatagac atatccctgg ccgccctctc ctaatttccg
     4261 gacgatttga atcgttccca tgttatcatc taattcaatt tgcattcctg cttgcaattc
     4321 agacattttt ctatctcctt ttccttagtc ctccgcagcg agaatccggt cgatttcccg
     4381 aatgatttcc agcctgcgtt tccgattttc attctctgca ttcagctgat tgtatttttc
     4441 ctctaaaata tgtgcctttt ccatgttgat gaacgaggtg aatgccgcag tttccagact
     4501 tttttgtaag ctgataatat cctgctttac cagatccaag aaaggaattt caatggaaac
     4561 cgcattcatg acctgttcgt ttaaaatgtt cttcaggatg cagacatcct ggttgaactc
     4621 gctcggaatc ttttcaattg tttttctgta gttctgaaag aactgcgcat atgctctttc
     4681 tgtcaactgg ctttcagcgg aatttggact ttcctgtagt gcatgttcca gcagctgatt
     4741 cagcttggtg actgctggat tttcctgaat ggcaaagagt ggaattgcat tggcatctgc
     4801 cgtttcaacc ggtgggagtt gagccagaag ggtgtccagt tcggcgagtg cattgttcgg
     4861 atgcttttca cagaagtttt gcagattgtg gtacagcggg ctgttaactg cgatcacttc
     4921 gtttgtcaca gattccacct ttacaattgc atcgttcaaa gaattgcttt gcgggtcatt
     4981 gttggaattg atttgctgat gaaataagat ctgatagtgc atcgactgtt tgtccggctg
     5041 gatctgcgga ttttcttcat ctgtaaacag agagatgctg tagaagttgc gtcggtcgtc
     5101 atcaaatttt ttatatcttg cgatgtttcc aaagggtgtg gaaatcaata tttcaaacag
     5161 gcttctggag tagcggcgaa tcaagctgtt ataatagcct tctggtgcaa ctttaccgca
     5221 ttgttcagag atgtactgtt tcagtgcttt ctccagctcc ttgaaaaaag gatgggaagg
     5281 tgtcagttcc agaccggatt caaatgcggt taaaatcttc ttttcgctct cacgatactc
     5341 ttccactgcc aaatgtacta cattgtcgat aaactcctca tacattgcag taaatttgct
     5401 gtcccgtatg gatgcgtcaa atcgctccaa aacgcctgca ccggagagtt cctcgtactg
     5461 tgctcttcga atctcgtctt cggtgatctg atagcgtaca ggatcgatgc tttgaattgc
     5521 atttttgcgg actttatccg tgattggctt gttctctttg cactgctgta caatttgcgt
     5581 ccggcactct gccagtgcgt tatggattct ctgccttgcc gttcttttta gttcgtcaac
     5641 cagattgtcc atttgcaatt ctatgctgga attgccgtcg atctgatttt cctgtcggag
     5701 ttcactgaat agtgcgagca cattttcctt taagttggca atgcgctgct gcatcacttg
     5761 caggcgaacg gtattgttgt agtcttcaag caggctgcgg atggtgtcga tgccgtctcc
     5821 ggcaagtcct ttttctttta ggctttgtaa aattgcactt tgcgtattgc cgttgtcaag
     5881 ttcaattctt gcccttgcgg agccggccgt caggcgctgt gcaatcaatg ccgttggcat
     5941 gattctgtaa ttttgcagtt cctctcgaat ccgctgcaaa ttatcctgga gtgtggttgc
     6001 aatatctgca cggtttgcga aaacaaatag cttttcccca atgaaaatgc cgtcatcgtc
     6061 tttgtccaca gattcaaaga actgtaccag agaatcattg aaactggggc gatctgcatt
     6121 gacaattaaa atgacagcat ccgatttttt catccacgct ctggtctggt ctttgtgtag
     6181 ttgtgtcgga gaatcgaaac ctgggacatc atatatgatc gcatttcgca tggcggaaag
     6241 cttatcggag cgtatcacaa tttccttaac ggcaagtgct tttgcaggat tttcgatgta
     6301 ttcctttacc tcggtttcta gttcatggtt aacaaaatgc agagattcag aaccgatcag
     6361 attttgaatg ctgctccgat ttgtgatgat ttcgaggatg tcgttataga tatttttctg
     6421 ttccgaacgc aagggtggca attgttcggt ttctttgtcc agcaattcct ttgaccattc
     6481 gctccaatgc tgcgggtttt caatgccgag aaggttcagc tttctgtgaa attcctcgtt
     6541 gaactgtgca tcggagtaga aatcgacctg tgcatcgttc tccgtgccaa accggatgct
     6601 ggttgcggtg tatgtgcacc gtgcgtcttt cgtcggaaga acatcattcc ccataaatgc
     6661 gtttgcaaaa gtactttttc cggatttttc aaggccaaca atggaaatct caaaggtatt
     6721 gttttttagc ttttcctgaa aaaaagtcat ttttttcaga atttcccgga gcttttgctg
     6781 ataagaaatt tcaattacct cactatattt tgtatccaat agcttttgta cacaagaaat
     6841 ctctgaactc agccgttcta atgtttcttc acgattcatc gatagctcgt cctttctgat
     6901 aagcatattt cttctcaaaa aaattatttt tcaaatggtg aatactatta accaccacta
     6961 tcctcattat atcatataat agatatgatg tcaaataata tttaccatat cttcaaatga
     7021 gggaaaccaa tatgttcaaa ctgaaaacca ccttgggcac caacaatatc tgcggaccga
     7081 atttgattcg gatccggcat gaaaaagggc tttctcagcg acagttggca aagaatctgc
     7141 aaattgccgg atatgatgta gaccaccact ttatccgccg catcgaaaat ggagaacgat
     7201 ttgtgacaga tatggaactg gtggcactgg caaaagtgct gcaggtggag atcacggact
     7261 tgattgatcc gacaattccc gtggcggtgc agaaagattc cctttgattc tattgtagca
     7321 aatgagcagg acaaacgtct gtccgttcaa agaaatgtta cggctgtaat aaaaaaagga
     7381 ccggcttgtc atgcagccga tcctttccgt tatgatttct tgggagtata tttccgaatt
     7441 tctgcaaaac acgtgtcaca gtagcctaat cgcttttccc aatgcagctg ctgttccgga
     7501 acgactttgc cgcagctttc acagcggcaa ttgccggaag cgtctttcca ggggtggtgc
     7561 agctttaaca ttcgcaaaaa tcgaagctgc catgttcctg gttgcaataa tgctcataag
     7621 cccagacaaa ctgcatcatg tccatttcgt cctggaagtt cgctgaagaa tacttcatca
     7681 gccggtttac atagaggttt ccggatttcg gagactgata gagatagatg gtaagcggta
     7741 caaagcaggc attgtgcagc tcacacagat cgatcagctc attggggttg tcctcttcat
     7801 agagcagccc aagcgtcgca cagaagtgct cgtaatcgcc acgccctaca gagcgaatgc
     7861 ggttgatgta atagtccctg tgtacgatgt gtcctctgtc tatgatagca acgtaacagt
     7921 atagtgttag ttcttccgga cgaaagcggt cttttttcag ctggatcttt tcgataaatc
     7981 cggcacaggg gcgatgcagc tgtagcggcg gaaaacgacg gtagttttgg aataatgccg
     8041 gaatctcccg ttctgccttt tgataagaaa cgctctttat cagcatgaga tctcgtttca
     8101 gctgcagacg ctgttccctg atctccggag caagatttgc catatctttc tcagtgcagt
     8161 aggtttccgg ctgaaaccag cggatctttt cgtagcagtc gtccagtccg aaagcaattt
     8221 tcagatctgt atcgccaaga ggtgtgatgc tttcaaagcc acggtctgaa gaaagaacag
     8281 cggtgcaaga tgttccaatg gcatagtcga gttccagtac gtagtcattt cgatgttctg
     8341 ttcgatcgga aaacaggtcg aactcctcgc agaaattctg gaatgcttct cgtccgttgg
     8401 taaagggctt gttgaaacat ttctgatgtt cggaaatttt gccgtcgacc gccagtgcag
     8461 cggttactgt aacactggga tacggttttt ccactgtctg aatgtctgtg atgatgccga
     8521 taaatgtgcc gtctgggtag ttgcaagtgt ctttcattga aaaaatctcc tttgtattga
     8581 tttgcagttt tttctgcctt tatagtatac catgccaaaa atccattttg taaaaacgac
     8641 tttgcaggag gcgatcgtgt gacggaagaa gaattgtacg caggtgcagg tgaggttacc
     8701 agaaagtgtt tggatgacat tctggaatcg gagtatggct ttgtgcagga cgatgaggaa
     8761 acttacacgt cggagtacct gctgacctat ccgtgcaaaa catttgcaga gggcttgacg
     8821 gatacgcttt tgcagcatgg attttccgga caggcagacg atgccaaaga aaagattgct
     8881 tatgttcggg aatgctgtaa gcagcgaggg gtaacgctca atccggaagt catcaaaagc
     8941 tggttctgcg gaactcgtcc taacagtggc gaacgcagcc gggacagtct gttccggctc
     9001 tgttttgctc tgggattgaa cgaccgggaa acggcttctt tttttcagaa ggtgtacttt
     9061 tcttgtccct tcaatttccg cagtgcaaaa gaaacggtga tctggtactg tctgcgaaat
     9121 ggactggggt atccggaaat gctgtctctg gcagagcagg cggaacagct gataaatgag
     9181 gaaagtgcga cagaggaaga aatgtgctat gagcagacca gtcagctgga aagtgcactg
     9241 ctgcaagttg gttctacaga ggaattgctt tgctttttcc gggaaaaccg tctggatttt
     9301 cagatgccca gaaagactgc cattcactat gcgaagtgcc tgatacagga agccacagaa
     9361 cttgctcaaa atgcagttgc agatcaaaat gaaatttccc accaaaagca gaaatccgga
     9421 aatgttgatc tgctgctgag tgtgatcttc ggggagaata tgcggactta ccagaaaaag
     9481 agttcctttt ccaaagaggc tgtttttccg gaattgatac gttctgattt tcccttggga
     9541 atgaaccttt ccagaatcaa acgaggagaa ccagtttcag atacgatttt gagaaagaca
     9601 ctgatcctgc tgaacttcta ttgtttcttt gcagtgctgt ttgcaaagaa tgacacatct
     9661 gatttgtttt gtgccattga gtctgatttt ctggactttg tccatgaaac caatgacctg
     9721 ttgtgcagca gcggtctgcc ggctttgtat gtgcggaatc cttatgactg gctgtttctg
     9781 cactgtgcca atcatcccta tccgttggaa gaactgcggt ctgccatgcg gcggtactat
     9841 tttgaccagg cagacgaaaa aagcacatca ctttccacac tgtgatgaaa gtgatgtgcc
     9901 tggatcatgt tgctgttaga acatggtctt gattttagaa ctgcatttgc aggtgtaagt
     9961 agaagtcacc gcggttacgg tgtaccatgc gtgcttctgt gccttcttcg gtcagaatgt
    10021 tttgaaacat ccggtgaaat tgaatgctgg aaatgctgca ggggaatcgg ttctcccggc
    10081 agtagcttaa aaatccggca tataccacag cggttcgctc tgcttttttg tgcttgggtt
    10141 ctggcaggac gatgcagtga ttgtaaaacg cctttaccac attgtgttcg tttttgtaat
    10201 gctccaatgc agcttcgcag gcaggagaat aggtgaaatt gtaatcgttg tcgatcagcc
    10261 gttccagtcc ggcataggca aaattgaaga tgcccgggag ttcttgcatc agatctcctt
    10321 ccagataaat gttcttgtag agaacaccct cctccggctc ctgattggga gcgaggtcgt
    10381 ggaaacactg ttcaaaggga atgatcagca gccggcgata gaacccgtcg ctgtaatcct
    10441 tggtctggat catatcattt gcaaggagaa tgaacttgct gtggatctct gtggtatagg
    10501 acttttcaaa tttttcttcg atttcaatgg aatcgccgcc ggtcagtgcc tttaggtcag
    10561 ctgtgctgta tagcttttct gtcttggtct cagaggaaat gttcagcagc tttcggtcca
    10621 ggttctgctt gccaaatctt ccgctgctga tctgatccaa cgcaacagca gatacattcc
    10681 attctcccag catatgcttg atcatgttgg ataacagaga ctttccgttg gaaccggaac
    10741 ccagaaagac aacaagcttc tgcataatgt cttcatagta taggcaggct cccatgatct
    10801 cctgaaccag atcgatccgt gtctggtcat tgtcaaagag ttcctgcaga aagcggaaaa
    10861 actgcggtgc ttttgccttg gggtcgtagt catatggaat ctgtaccaga cagaaaatgt
    10921 tcggcgttgg tttctctatc gtttcctcct gtaaatcgaa gatgcagttt tggaattgca
    10981 gaagtcccgg acgcattccg ttatcctctg catagggaac ttttcgctta aaatagggga
    11041 tatactcgtt ttcctgcttt cgtttccaga tattgcagga agcttcttca atgatcgcaa
    11101 agaaaatctt tttgtatgcg tgatcttcca ggcattcata acactttgac ttgaaattgt
    11161 agaagtagag ctttccattg cagtataatg cgtggactct tgtcattgca tactgaatga
    11221 atacggtgtg gtcg
//
LOCUS       DAWBGR010000073        11201 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1709_length_11201_cov_4.452808, whole genome shotgun sequence.
ACCESSION   DAWBGR010000073 DAWBGR010000000
VERSION     DAWBGR010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11201)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11201
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1709_length_11201_cov_4.452808"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2888 a         3055 c         2917 g         2341 t
ORIGIN      
        1 gcataaagcc ctcctgacgg ctttgcacgc aatctgcgtg caggttttat aaggcaatac
       61 cataactgca ccgaatgcgg cggatctgca tacactggaa tgcaccggat tctcgaaagc
      121 ggtgcagaaa ggagttttgt aatgcaaagt tctatggatc acatgccgca gatggcaggc
      181 atgcagcgga acttcctgag ggaattccag tcggctgatg ccccgatgta cggcatgcag
      241 gaagcagaac cattccaacc caccacgccg tttccggagc agacaccgcc gggcatggca
      301 tatgtcccct atcagcagtg ggaggagccc tatgatgcag acaccgcctt ccctgtcgga
      361 accatttttc cggcactgga ctatccctat cggggaggtg gttcctgtgc atgagaaaca
      421 aaagctgctc tccatgattc ggaaatatga tttcgtcctg tacgaattac agctgtatct
      481 ggacacccac ccccgctgtc cagaggctct gcgaatgtgg aaaaactatc aggcaatgcg
      541 gcagaaagcg gcaacggcat atgtccggca gtacggtccg ctgcagcctt tgcagaccag
      601 cggtgacagc ccctgggcat ggaatcaagg cccgtggccg tgggaaaagg aggcgaactg
      661 agatgtggac ctatgaaaaa aagctgcaat accccgttcg gatcaaaaca cccaacgcca
      721 gaacggcgaa gtatattatt tcacagcttg gcggcccaga cggagagttg gctgcttcgc
      781 tacggtatat gcaccagcga tacacggcaa aacctgacaa agtaaaagga gttttgacag
      841 atattgccac agaagaacta gcccatgtcg agatgatttc cgcgatcctg taccagctga
      901 ctgacgggct gacaccggaa cagatcaagg aagccggatt cgacgcgtac ttcgtagacc
      961 acaccctcgg actgtacccc cagagtgccg ccggcgtacc attcacggct gcctatttcc
     1021 agtccaaggg ggatccgatc accgacctga ccgaggatat ggcggcagag caaaaggcac
     1081 ggagcaccta cgacaacatt ctccggctgt gcgatgaccc cgatgtccgc gacccgatca
     1141 agttcctgcg gcagcgggaa atcgtgcact tccagcgttt cggcgaagcc ctgcggcaag
     1201 tgcaggacga cctgaattcc aagaatttct actgctgcaa cccgtctttc gacagcagct
     1261 gcggcaaaac cgcctgccgg agaaaaatgc ggtaacacac ttcccataag gcaatgccgc
     1321 acaaagattg ttcggtattg ccataacttt cagatgctgc tctgtttccg gagcagcatt
     1381 ttttctgttt ccctgaaaag ttcacatacc actccttgac ttttcccata tttatggtat
     1441 actagcatac ggacagtgtg attttcgcct gtccatagaa gaagggagaa gtgggtatga
     1501 tacaactcat tgcaacagat atggacggaa cgctgctgga cgaggagaag cagcttccgg
     1561 cacagctgcc tgcactgctg gaggaattat atcgccgcga tattacattt gccgtggcaa
     1621 gcggcaggtc gcacatgatg ctgaccaacc tgttcggaga acttgccgaa gaaatcattt
     1681 tcatctgtga caacggggca tgcgtcatgt atccccatga agcaccgctg ctgcacagtc
     1741 tgccaaaaga tgtcattcat cggattctgg acgcgtgctg cagcctgtct gccggtgtgc
     1801 cggtgctgtg cggattccac cacatttact atccggaaaa cgacaacgct gccatgcagc
     1861 atgaaattca gcggtattac cgcaatccgc aggtggttcc ctatgacgac ctgtacgctg
     1921 ttgacgaacc gatcctgaaa gttgccctgt gcgacaccaa cggaccgaca aaggaagcct
     1981 accctgtgat gcacgaagca ctgggcgatg cctatgaact gctgatctcc ggcgactgct
     2041 ggatggatat tatgtgcaag ggtgtgacca agggtgccgc cttgcaggga ttgcaggagc
     2101 gtatgggcat cacaccggcg gaaaccatgg cattcggtga ctatgacaac gacatttcca
     2161 tgctacagca tgcggaattc agctacgcta tggaaaacgc ctcggagcgt gtccgccagc
     2221 aggcaaagca cattgctccg gcaaacacca aaaacggtgt ggtgcgggtg ctctgcgaaa
     2281 cactggggat ccatatggaa tcgctgtgag agtgcggctg cccataaacc aaaaatgaaa
     2341 ttcttagggc aataccgcac gcaatctgct tgcagtgttt cccttataac agagggacgg
     2401 tgccggcatc gtccctctgc gtttctttcg ctgcaaaatt tttggcaata ccgcacaaag
     2461 agcgtctggc gactttgcac gccatctgct tgcaaatttc ccttcagagc ctgtttcgta
     2521 tcttcccgca cgcatctgtc gcacaatctt tgtgcggtat tgccttttca tttcacctgt
     2581 tttttaccgg cactttgcaa aaaaagcttt acaaaaccac aaaaatatgc tatactgtcc
     2641 ttaacgttct gatgatatgg aacgtgaagg aaacactaca tgtcagaccg caatccggca
     2701 tgcggtttta cgtttcctga aagcactttt tgcatttgtc gtcactgaca gaaaggagtt
     2761 ttggaatgga agcatctatg ccatttctgc tgtggggcgg cttgtttgtg ctgacggttc
     2821 tggcggaatt cgccacccag cagctggtga gcatctggtt tgctgccggt gcactggcgg
     2881 gctttctcgc agcctgtttc ggggcttcgc cggtggtgca gatcgtcttg ttcgtggtga
     2941 tctctgtggt acttctgatc ttcacccgtc ctatcgtccg ccgaattttc tcgttcggca
     3001 tcaaggacac caacaatcag gagatcggca ggatcgccgt cgtcattcag acggtagacc
     3061 ccatacacaa caccggacgc gtccggctgg acggagtaga ctggatcgca gtttctcagg
     3121 acggcacagt cattccggaa aacaccagcg tccgcatcga agcgatcagc ggcaccaagc
     3181 tgctggtcag ccggctgccg cagacagaag aacaaactgt aaacaattaa aggagaacac
     3241 acatgggact gattatttta cttctggtta ttttcatcat tgtgattttt gtcacacgaa
     3301 ttcgcattgt accgcaggca aatgtctacg tcgtagaacg tctgggtgca ttctataagg
     3361 cgtggggcac cggtatccac tttctggtac cgttcatcga ccgcgtggca aagcgggttt
     3421 ccattaagga acaggtcgta gacttcaagc cgcaggcagt gatcaccaag gacaacgtta
     3481 ccatgcagat cgacacggtc gtattcttcc agatcaccaa ctcggtacag tttgcctacg
     3541 gtgtggaaca ccctatcgca gcaatcgaaa atctgactgc aacgaccctg cgtaacatca
     3601 tcggcgaact ggaactggac gcaacgctga cttcccgcga cctcatcaac acccgcatca
     3661 ccgaaacact ggaccttgcc acagaccgct ggggcatcaa ggtcattcgc gtggaactga
     3721 aaaatatcct gccccctgct gaaatccgtg acgccatgga aaagcagatg aaggcagagc
     3781 gtgaaaagcg tgaaaagatt ctccgtgcac agggtgaacg ggaatatcag gtcactgtcg
     3841 cacagggtga aaaggacgca aagatcctcc gtgcagaagc agaaaagcag actgcgatcc
     3901 tgaaggcaga agcggacaag gaagccgtga tcctgcgagg cgaagcgatc aaggaacaga
     3961 aaatgcgtat cgctgtcggt gaagcacagg caattgaact ggtgcagaag gcactggcag
     4021 acagcttggt caagctgaac gcagcaaatc ccagcgaagc tgttctgaag ctgaaatcgc
     4081 tggaagcatt cgaaaaggca gcagacggca aggcaaccaa gatcattatc cccagcgaga
     4141 ttcaggggct ggctggtctg gctgccggtc tgaaaggtgt cctctctgac agccctgtaa
     4201 ccgcaacagc tcctgcaccg gctccggctg gaaaaacggc tccggcagca gaggctccgg
     4261 cggaaaacca gtaagctttc tgaggcagta cggtacaaag gcaataccgc acaaagtctg
     4321 tgcagattgc cttgattttc catgaaaata ttgtgactaa aaaggacggc ttcgtgccgt
     4381 ctttttttgt ggaaaaaaac gattttccgc gaatgcgaat tcttggggct gttccccttg
     4441 caatcctgtc gcaaattgtt gtataatagg agttacgaga gaaacgcgga acaaaagcat
     4501 gcccgccatg cggcgtttct ccaaagtaaa ggcaccaccg tgattcgatg ccggatcata
     4561 tttgatattc tgacagaaac agaggttata cacatatggt tttttcccga ttgtttttca
     4621 tttacctatt cttccccatt tgttttctct gctattttgc gacaaagaaa acctcttacc
     4681 gcaacgcagt gctgatcgtc ttttccctgt tgttctacgc ctgcggggat atttcctttt
     4741 tgtggctggg cgtcgccagt gcactggtca acttcgtgct ggggcgtggc atcgacaaat
     4801 tccgcgacaa ggctcccgca aaggcattct tggctctggg actggtgctg aatctgggta
     4861 cattggtggg atttaagtac accggctttt tcattgagaa cctgaacaat tggttctccc
     4921 tttccattcc cgtccccgac ctgccgctgc cgctgggcat cagctttttc acgttccgca
     4981 cggtttccta tctcatcgac tgtgcatggg gcaaagtcaa ggcggaacga aatttcttca
     5041 cgttcctgct gtacgtttcc tttttcccga tcaccatttc cggaccgatc gcacggtacg
     5101 aaaccatgca ggcagacctg caccagcggc gggtgtctgc cgacgacctt agcattggct
     5161 tcggacgcat tgccgttggt ctgggcaaaa aggtcatact ggcagaccgg ctctccggca
     5221 tcgtagacca gttcctgggg aacgacttca ccacccagac cacactgggt gtctggtacg
     5281 gtgtaattct ctacgccctg caaatgtact tcgatttctc cggctattcc gatatggcaa
     5341 ttggcattgc ccgcatcttc ggctttcgtc tggacgaaaa cttccgccac ccgttcctct
     5401 gcaaggacat cactgagttc tggcagcgtt ggcacatttc gctgggaacg ttcttccgcg
     5461 attacctgct gtacgtaccg ctgttcggca aacgccggaa aatcggcggg ctgctgctgg
     5521 tctggttctg caccggtctg tggcacggtg ccagctggaa ctatatcatc tggggcattt
     5581 actacggact gttcctcatg ctggaaatgg gcatcggcaa aaagggcatg aaaaagattc
     5641 cggcagtgct ccggcatctg tgcaacaaga tcgtcatcat tgtcgggttc ggcatcttct
     5701 actgcaccgg cggtatcgac cagctggggg atatgctgct ggcaatggca ggacagaacg
     5761 gcaccggctg gctggacacc atgctggtca cttccgtgca gaacaacctg ttcctgctga
     5821 tcgctgccct gctgtgctgc ttcccgctgc tggaactgcc gaaaaagatc atggcaaaaa
     5881 atccggctgc caatgttgcc ctgtccgtca ccggcacagt gcttgccgcc ggcctgctgg
     5941 tcatcagcag cattctcatg gtagatgcca ccaacacccc gttcctgtac accaagttct
     6001 gatccaagga gggagaacac catggacgaa aacaacaagc cgcaaagtga cttcgatctg
     6061 gacgcggcac tggcagaaaa atttcagcag gtacagcagg acgcaaaccg tctgcgtgcc
     6121 atgaatgaca ataccgatac accggcttct tcgcctgttc cggaaccgac tgaggtgccg
     6181 caggaacagc cggaaccgga aaccggatcc cctgctccgg agccggaatc accggaagaa
     6241 ccggcagagg aattgccaca gttcgacatc ggggaaacac ccgaaaaacc ggctgctacc
     6301 accgaggagg acaaagccgc agccaaagag cgaaaagccc gctacaagct gaaacggcag
     6361 ctcctaatca ccaaccgcat cggtgccgtt gtgcggatct tcgccatagg aacgatcatc
     6421 ttcggcggct cgatcttcct gctggtgggc acacgcccca cggaatccgc cgaggaaaac
     6481 cggaagcttg ccacgccgcc cggcttctcc ctcgaagcac tgggcagcgg cgactatgtg
     6541 aaagacctga tgcactatta cgaggacacc gtccccggac gcagcagctt caagcacatg
     6601 atcagcaaac tggaaagcta tcagggctta cagggcgagg acaaggtaca gttctacggc
     6661 aacattgccc ccgtcaaaaa agaggaaacc gccgatgcgg acagcaggac aacgacgact
     6721 gcaccggttg ccagcggaaa ccgttccaca gaaaccacca cggcaactac cgttgtcacc
     6781 gagccggagc cggatcctgt ggagatcggc gatggcattg tgctggtgga caagcgggcg
     6841 atcagcattt acggcggcag cttctcccgc ggggaaaagt atgcggcaac gctgaacgaa
     6901 tacaagaaac agctgggcag caaggtcaat gtctattcgc tggtggctcc tacggcagta
     6961 tcgttctacc tgccggattc ctacgcgggc tacaccggca gcgaacccga caacatcgac
     7021 cacattgacg aaaacctgaa agatgtcaag gcagtcgatg cctatgcagc actggcggca
     7081 cacacggacg aggacatcta tgcccgcacc gaccaccact ggctgccgct gggggcatac
     7141 tacgccgcag aagcctttgc caagacggca aacgtcagct tcgctcccct gtccggctac
     7201 accaagacta ccctgtccgg ctatctgggc agcatgtaca cgttcaccaa gagtgccgtc
     7261 ctacaggaga atccggagga cttcacctat tatacgcccc acaaccagta caccacccag
     7321 tattatgaca ccaacttcac caacgaacgg gacggcaagc tgctgatctc gctggacaac
     7381 gtggagccgg tcagctggta tctggtgttc atgggcggcg acgaaaagat cacccacgtg
     7441 aaaaccgatg tcaccaacaa gcggacactg gtgatcgtca aggacagcta cggcaacgcc
     7501 cttgtccctt atctcacgca gtccttctcg gacatctacg tgattgatat gcgttacttc
     7561 gacctgaacg ccgtttcctt tatgcagcag gtcggtgcaa cagatgtgct gttcgccatg
     7621 aacacgttca gtgccaccgg cggcaacagc gaatgtctgg aaacaattcg gacacagtaa
     7681 gaggtttcac aaatgaaaga actggaatat ccctttgaca gcagctttct gctgcaaaaa
     7741 cgacgcaaac tgaaaaaagc acttctggca gacggctctg cccgcatgga aaaacgcatt
     7801 gccgtgctgg gcggttccac caccaacgac attgtttcca tgctggaact attcctgctg
     7861 aactgcggca ttcagccggt gttctaccag tcggaatacg cacagtactg gcaggatgcc
     7921 atgttcgatg ctccggaact ggcagcattt cagccggatc tgatctttat ccacaccaca
     7981 agccgcaaca tcaccgaagc cccgctgccc ctgtccgctt cagagcagga agtacaggac
     8041 gcactggaac ggcagtacac ccacttcacc cagatgtggg agcacctcgc ggcaacatac
     8101 cactgtccca ttttacagaa caactttgaa ctaccggcaa cgcggctcat gggcaaccgc
     8161 gacgggtggg actgccacgg catggtgtgg ttcatctcgg aactgaaccg gaaattcgga
     8221 cagtatgctg ccgcacacaa cgacttctac attcacgata tacactatct ggcggcaagc
     8281 tacgggctgg acgcatggca cgacaattcc tactggtacc tctacaaata cgccatgacg
     8341 ctggacgcga ttccggaatt cgcctacagc ctgacacgaa tcattcgctc catttacggc
     8401 aagaacaaaa aggtgctggc tctggacttg gacaataccc tctggggcgg tgtcatcggc
     8461 gatgacggac aagagggcat ctctgtcggg caggaaaccg ccgaagcgga ggcatacacc
     8521 gaggtacagg aatactgcaa ggcacagaaa cagctgggcg tacttctcac gatctgctcc
     8581 aaaaacgaac cggaaaacgc cagactggga ctttcgcacc ccgattctgt cctgcacgag
     8641 gacgacttcg tggcaatcaa agccaactgg gagccgaaag accggaatct ggtacagact
     8701 gcggcggaac tctccctgct gccggaaagc ttcgtctttg tggacgacaa ccccgcagaa
     8761 cgggaaatcg tttccgccca gatcgccggc attgccgtgc cggaatttca ggatcctgcg
     8821 gactgcattc gggtgctgga tcacgcgggc tactttgaaa ccaccacgtt ttccgcagca
     8881 gatgccagcc gcagcgaaat gtaccgtgcc aatgcccagc gtgcccagat gcagcagacc
     8941 tatgccaact acacagacta tctgctgggg ctggatatgc acgcagacat tcgggatttt
     9001 gatgcggtag cactcccccg cattgtccag ctgaccaaca agagcaacca gttcaacctg
     9061 accacacggc ggtataccca gagcgaaatg gaacaggtgg ctgccgatca caagcacatt
     9121 cgcctgtacg gacggctgcg ggacaagttc ggcgacaacg gcattgtttc cgtcgttatc
     9181 ggagagatct ccggcgacac cctgcacatc gaactgtggc tgatgagctg ccgtgtactg
     9241 aaacgggata tggaactggc gatgctggac acccttgtgg cagagtgccg gaagcagggc
     9301 atcaccgcca tctgcggcta ttactacaag accgccaaaa acgccatggt caaagacctg
     9361 ttcgggacat tcggctttac gaaaaccgaa cagttcgaga acggcgacag cacatggcgt
     9421 atggaaatcg ccggctatga aaaccagaat catgtgattc aagtcaacga aaaggagcat
     9481 cagcaagcat gacacacgaa gcgattatgg agaaactgaa cgaaatcttt caggacattt
     9541 ttgacgatga cacgctggtc atcacagaga acaccactgc caacgacatt gaggactggg
     9601 acagtctgga acacatcaac ctcgtcagtg ctgtggaaaa ggcattcggc atgaaattca
     9661 ggatgcagga agtttccggc atgaaaaatg taggcgaaat ggcagacatt attgctgccc
     9721 gtgccgaaga accgaagccg aaaaagcggg gattgttccg gcggtaagcg ttttttgctg
     9781 ctggaagaac aaaaaaattg gcaacaaaat acaaagaacg cctgaaatgt gcagaaatag
     9841 ctggacattt caggcgtttt gctgtataat gatgatagat ttgtatacca ttcgaatcca
     9901 ctcggaggaa tccagatgcc aaaagaacag tttttatctt atcaaaaaga aaatcatcac
     9961 ccgtcacagc caccggtact ggacgctgtc gggtcggttc tgaatgccct gtgtgtcacc
    10021 aaaggataca cttggaaaga ggcgtactgc aagctgatcg ccgtcgcggg aaaaatcggg
    10081 cagatgcccc aatatcccaa aaccattcgg gaactgctgc atgaagaagg gttctttttg
    10141 caggcaaaaa ccaatgtcaa caagtgtata cgggagatca ttgccgattg caaccacagc
    10201 tttcatgacg gcgaagtggt catactgaat ttgtccgtcg gacacacgaa cacggacgac
    10261 ggggaatatt gtcccctcgt gccccatgat ctggacggac agacaaagta tgccctgcac
    10321 ttcccgcagg acaacagaga tcggatcgcc cgtgaagtgt gggttgcctg gaaagacgga
    10381 caggatcaca gccccctgcc gcagcagcag agccgtacac agagaaaaga gctgaaactc
    10441 cacatcgaag aaaatgaatc gttggtagtc ctgaacgaaa atcccaacga caactacatt
    10501 ggagattgtg ccgtcagagc ctttgcagca gttctggaga tcccgtgggc agaagccata
    10561 aaaaggcttg ccgaagctca aaactacgcg gcgaccattc tcaatgggaa aaaaaacatc
    10621 gatgcccttt tgaaaaaaga ggggttcgag aaattcgatg ccatgaagcg aaacggaaag
    10681 atcctgaccg gcaaggagtt ttgcagcctg attcacgaca tgtttccggc gggaacaaga
    10741 atatacgcgt attcgggaag aagccatgta gttgccattc tggtgtttga cggggaatac
    10801 aaaatcgtgg acacgtggga cagcaccaac cgaaaaatca tagaatactg ggcaaaatat
    10861 ccccaaaagc ccaaaagacc gagaaaaaca gaagcaccga cagaaaaact cacagcactc
    10921 tctgtcggca tgaccattca gcacaagacc ttcgggaacg gcaaagtgac tgccctttct
    10981 gataccattg ccaccattca gtttgccggc ggtgtcgaga aaaagtttgc agtcgcttgg
    11041 gtgctgggga attgcaaacc atgtgaaact gcctgaactt ctaccgaaaa agagaaaagc
    11101 tggtattcta tgcaagagaa taccagcttt ttgtattcag tattgccttt aggcaatacc
    11161 gcacaaagag cgtctggcga ctttgcacgc catctgcttg c
//
LOCUS       DAWBGR010000074        11087 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1727_length_11087_cov_7.410986, whole genome shotgun sequence.
ACCESSION   DAWBGR010000074 DAWBGR010000000
VERSION     DAWBGR010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11087)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1727_length_11087_cov_7.410986"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2524 a         3017 c         2687 g         2859 t
ORIGIN      
        1 tgcaagcaga tggcgtgcaa agtcgccaga cgctctttgt gcggtattgc ctcaacattt
       61 tatttgtatt tctgcgttga tttccctgct ataaaaagag cgtcctgcaa ccacagaacg
      121 cccttggatt ttctgttgga gatgagtctt agttctccac aaccaccttg tggaatacct
      181 ttttgccctt tcgcagaatg gtttctgctt ccggatagat cacgctcttg gggtcggtga
      241 ccttggtgtc attcacagaa acgccgccct gctgtaccag ccggcgtgct tcgccgttgg
      301 acggggacag tccgcagcgt accatcaagg tcaggatccc gatgccgccg tcggtgagtt
      361 cctctgcttt cagaacggtg gtcggcatat ccgcattgtt tccgccgccg ccaaagacag
      421 cctttgccgc agtctgtgcc ttttcggctt cttccttgcc gtgcaccagt gcagtcagtt
      481 catacgccag cagttccttt gcctgattca gctgctgtcc ctccagtgtg cgttccattt
      541 cctcgatctg ctcaatggga acaaaggtga gcagcttcag gcacttgata acgtcctgat
      601 cggaaacgtt ccgccagtac tggtagaact cgtacggtgt cgtcttttcc gcatcgagcc
      661 ataccgcacc cttttcggtc ttgcccattt tcttgccctc ggagttcagc agcagggtaa
      721 tggtcattgc gtaagcgtcc ttgcccagct tccggcgaat cagctccgtg ccgcccagca
      781 tgtttgccca ctggtcatcg ccgccgaact gcatgttgca gccgtaatcc tgaaacagct
      841 tgtagaagtc gtagctctgc atgatcatgt agttgaattc caggaatgtc agaccgcgtt
      901 ccatacgctg cttgtagcac tcgtgagaga gcatgcggtt tacgctgaag tgagcaccca
      961 cgtcgcggag cacgtccaca tagttcagat ccagcagcca gtcgccgttg ttgacaacaa
     1021 ttgccttgtt ttccgaaaaa tcgatgaacc ggctcatctg cttcttgaag cagtccacgt
     1081 tgtgctgaat ggtttccttg gtcatcattt tccgcatgtc ggttttgccg gaggggtcgc
     1141 cgatcattgc cgtgccgccg ccgatcagaa cgatcggctt gttccctgcc atttggagcc
     1201 gcttcatcag gcacagtgcc atgaagtgtc ccacgtgcag gctgtctgcg gtgggatcga
     1261 agccaatgta aaaggttgcc ttgccggcgt tgatcagttc ctcgatctcc ttttcatcgg
     1321 tcatctgggc gatcaggccc cgtcgtttca gttcttcaaa tactgtcatg ttctttccct
     1381 ctttccccga agggtacaaa aaagccctcc ggaaactatt tttccagagg acgatgtttt
     1441 caccgtggta ccacctcaat ttatcagagc gaaagccgcc ctgacctcaa agacgctgta
     1501 acgggcgtgc ccgaactccc ctacatacga cggtttcagg aagcctgctc ccaagtgtat
     1561 tctctgcggt gacgttcccc cgttccacca acccgagggc tctctgtgcc ggcacgaatg
     1621 cagatacttc tcttgctcta cgcatttcat aatgtgttta tcataccata ttgtcgggcg
     1681 gttgtcaagg gatttttctg taaaaaatcc agaacgccta cttgacaaat ccaaaggaat
     1741 cgtctatcat agggcaatac cgcacaaaga gcgtctgggg actttgcacg ccatctgctt
     1801 gcaaatttcc cgtcatattt cacaaaaatg ctcgtgaata catcaagtat tcactccgct
     1861 ttttgtttca tctgacgaaa aatttgacga cgcatctgtc gcacaatctt tgtgcggtat
     1921 tgccatagaa atagaaagag cataccagta gacggtttgc ttccaaagat tgctatatga
     1981 aaaaatagcc gccttatgcg agagtggggc ggttattttc atttatagcg gtaaaatatg
     2041 cttgacacga caccattccg gtgctataat aaaggcgtac ggggcattag cacagttggg
     2101 agtgcgtcac attcgcattg tgaaggtcag gggttcgatt ctcctatgct ccaccagaaa
     2161 cccaccaaaa ttccaagaga attacggtgg gcttttctat accatttctt acatcaaaac
     2221 ttgcgatgca taattaggga gaaaaaatga aattcaaaac gataaacacc ataatatgtt
     2281 ccgccacaat gtcaattatc acagtgtcag ctatatttct ttcggaaatt atcggtatcg
     2341 caaacgagtt attccgattg ccatatttgt tggttgtagc agtaatatat gcagctatgc
     2401 tgttaagtga aagtaagaag cagcttctgt tgaaatgggt tttgtcactg tctttttcct
     2461 ttttttgctt cgaatatttt tggcaaacac actattctat cagagcgtta aactggataa
     2521 tagagggata tggaacacaa tctgccggcg gcaatttttc aggttttata gttcttattc
     2581 ttttgctagt cctttgcttt gcaggcatga tatttgcata ttctaagtca tctgaaaaaa
     2641 tcaaacgata tataaaagtg cagtcattga caggcatatg gattttcatg ttaatgatta
     2701 tcgtagtcgc atacttggaa acccaatttc cagcatatca cgatgtatta tcatatcatt
     2761 aaacaccaca caaagaaacg gctctgccac aggaatttcc ctgcggcaga gccgtttctt
     2821 gttttcagcg tatcttaggc aaaccaacga ttatgtctgt ttgccgtccg ctttcttttt
     2881 cttttcaatt tccgcctttt tggctgcaaa ttccttgtcg tccagaatgc cctgttggtg
     2941 gagtgcctcc agtttttcca gcagcttctc tggagagtcg tcatttgcct tgtccttggt
     3001 accggacttc agcttcacgc gttttttcgc ctgctgctgt tcccgttcct tttcagcttc
     3061 gatctctgcc agcattgcag cataccgctt gcggtcaccg aaccagaaca gcacgatcag
     3121 gataatgccg cctgccagtg tacccatgcc gatccaccac cccggcggtg tgtaggtcat
     3181 ggtcacggta tgttctccgg cagacagcgg cacaccgatc atggcatcta cgattgccac
     3241 ctgatcgacc ttcttgccgt ccaccttgac ggtccagccc ggctgatccg gaatggaagt
     3301 catcatgatc tgaccgtctt ctgcggtaat ggtaccggtc agcttccggt cgttgctctt
     3361 tttggtatcg atctgccact gattctgctt cagaatgtca atatcctctt ggaatacgtc
     3421 cggatgatag gtgtagaaca ggaagttctt gacaatggtg aacttttcct cattggcaat
     3481 ggtcatacgc agggtgatct tcgtgcccgg ctcaaaggta cccagacgca gggcaatgta
     3541 gttatcgccc tcgaagtatt cgcccataga ctggaagcca ccgttgttct tggcgtattc
     3601 atcggcatct ttccaagtga agtcgccgct gttagattcc ttccagccgt tttctgcctc
     3661 gctccattcg ctgtcgttcc atgtggacat ccacaagttg actttcttct ggaactcggt
     3721 cttgaagtag atgtagatcg gttcgttgga ctctgccacc aagtggaagt ccacagtggg
     3781 gtcgccgtcg ccggtcacgt catacttggt ctgttcgccg taggggcttg tggtgattgc
     3841 atcgctgacg atcggatttt ctgccagatt cagcggtgtg taatactcgt gccagctctg
     3901 gaactgtccg ttttcgtcaa atgtggtctg tccgctcatg gtgctcatga agatgttctg
     3961 gctgttgaac ggattgtcgt tgcccagatg atcgatcttc tgcacatcct cgtctaccat
     4021 gtagccgata gacagagcgt tggtattttc ataagcggta atggtctttg cagtaccatt
     4081 ttcgttggta tagtcgtatg cccagcgtgc ttcatatgcc ggattcagac ggcggttctg
     4141 atcaccgctt tccccgcggt ccagcacata cttgataccc agcagagagt ccgcaatttc
     4201 ggtgttgccg tcatagcggg tatagtagct gtgcatgcag taacccaagg tttcaatgaa
     4261 attgatggca cgggtattca tgacagaact ggagtgtgag atacctttca gtccgaatgc
     4321 tgcattatcg ttgacacagc ggaagaaggt cttttccgca cggtagaagc cgtcgtcctt
     4381 ttcctccagc atctgtgttg ccgcacgtcc gttctgcacg tagttctgat aggaggcacg
     4441 ggtggagtat gccacttcct tgtcgatgtc cttcatggaa tctacagcgt tgtaggttgc
     4501 ttcgccgcct accatgatca gcatcatagt ggtcagggca acgcttacgc cacgcttgcc
     4561 ctgcgtaatc tttccgccca gcatgaagta cagcaggatc aggtaaatgc ccatgagtgc
     4621 cgcagagatc cagacagcac cctttgccat ctggtcaaat ttcagaccgg caacaatggc
     4681 aatgatgacc gccattatca ctgccgatcc gatcagatgc ttcttttcga taccgtcgcg
     4741 gtttgcgaat gcagttgccg ccatggtcag gaacacaaag gacagcagga atgcatagcg
     4801 gaacggcagc cagttcggca cctgtccgcc gtgccacatc atgtctatcg gtgtgatcag
     4861 catgcacatt accattacag cgatcagcag agaataaccg atcttctttt tccggttgac
     4921 ctttttgttc aggtagaaca ggggcagcag aaccagtgtc agcagaccgc agtagatttc
     4981 cggcttgccc tgtacgttta cagagtcata ctgtgccggc agcagctgcg ggaacatgtc
     5041 cagcaggtcg cacaggaata ccagcagcgg cgaatcctgt gcattttcca gcagtttctt
     5101 gctcagcggg aacgactgga accggaagct gtagtccgga tctgtgaatt cgaacttacc
     5161 cagtttcaga gcgttgtaca ccggcagcag catgaatgcc gcacatgcca gtgcaatgac
     5221 ggaagaatac gcgatccgta caaacgcggt gaaatagtcg taacccttcc gcttcttgtc
     5281 cgagccgaag cacagataaa acagaaagta caggatcacg aagaagcaga tcatgtagcc
     5341 gatgtagaag tttgccacac acatcagtgc cagcggaatg atgaaattca gccggcggcc
     5401 gtcatcgacc agatattcaa tgcccagcat gatcagcggc aggaatacca gaccgtccag
     5461 ccacatgggg tcgatcagct ggatcacgcc gtatgccatc atcgcataca tggtggaaaa
     5521 gagcagtgac gggtacggct gtacgccgcg gcgtttctgt aagaagtagt taaatgttgc
     5581 accggctgca cccagcttgc acagctgcat gatcagcagg ctgctcagca tgaatttctc
     5641 cggcaaaagc atgacgatca gcgtgaacgg gctcgccaga taatagccga taatgcccat
     5701 aaactcgccg gaaagatttc gcgaccagtt gtagaagatg ctgccgtcac cccagaacgc
     5761 atcacggagt gcctcgaaat agtacacata ctgtccgttc aggtccagac agagcacgga
     5821 ttcttcgcca aaggggtaga gtccgaaaag ggcatatgcc agataggtca gcacagccgg
     5881 aatggcaaag gcgatgagat agatccggtt ccggacgatc catctgccaa ttgcagagat
     5941 gacactgccg gcactgaaac tgctgcgttt tgcagtctgt ggggcagctt ggattttggt
     6001 ggttgccatg aaagtcctcc ttaaatgata agatgatcgc cggattccag caacagaatc
     6061 cggaaagtgt gttccgccgt tcccctcctg cggacagaac aacagatacc aacattatag
     6121 cacaatcttt cccgtttggc aagccctttt tatgcaaaaa acacggaaaa gtacgaaagc
     6181 catatcccaa aatccataaa aaccacgaaa gccgcctgcc ctgcaaatgc aaaaatgccc
     6241 cgcacagcac aacgctgtac gaggcatttt caagagaacg ctgtcacaaa gccgtcaggc
     6301 ggactttgta cggtgttgcc tatattgata cgatcttatg cggtctgggc aaattgtgaa
     6361 tgatacagat cggcatagaa gccgttcttc tccagcagtt cgtcatgggt accctgctcg
     6421 atgatgtccc cgtccttcat gaccagaatc atatcggcat tgcgaatggt ggacagccgg
     6481 tgagcaatga taaagctggt tctgccctcc atgaggtgat ccattgcctt ttggatcaga
     6541 atttctgtac gggtatccac agaggaggtc gcctcgtcca gaatcatgac ccgattgtcc
     6601 gccagaatgg cacgggcaat ggtgagcagc tgtttctgtc cctgtgaaac gttgctggca
     6661 tcttcgttca gctccatctg gtagccgtcc ggcagctggc ggataaagct gtccgcatgg
     6721 gcagcctttg cggcggcaat gacttcttca tctgtcgcgt ccagtctgcc gtaccggata
     6781 ttctccataa tagtaccctg aaacagccat gtgtcctgca acaccatgcc gaaagattcc
     6841 cgcagcttgt ggcggtcgaa gtcccgcaca tcatggccgt ccagcaaaat cgcaccgccg
     6901 ctgacatcat aaaaccgcat cagcagtttc accattgtgg tctttccggc accggtgggt
     6961 cctacaatgg cgatcttctg tccggacttc accttggcac tgaaatcatg gatcacgcac
     7021 tgctccggct ggtaaccaaa ggaaacatgc tgaaaatcaa cattgccctc cacgtttgcc
     7081 agttccagcg ggtgctctgc tgtctgcact tcttccgctt cttcgaggaa ttcaaagaca
     7141 cgttcgcttg ccgcagccat agactgcacc tgattgagta cctgtgcgat ctgctgcatg
     7201 ggctgggtga agttcttcac gtactggata aacgcctgaa tgtcaccaat ggtgatcgtg
     7261 ccgcgaatcg ccagcagacc gccggacagt gccacagcgg cataacccaa attgcccacg
     7321 aacatcatga tcggctgcat caagccagac agaaactggg atttccatgc ggagtggtac
     7381 agggtatcgt tggtttcctg aaactgctgc accatgtcct tttccttatt atacgcctga
     7441 atcactgtat gaccggcgta ggtttcttcc acttgtccgt tgatgtgtcc cagatattcc
     7501 tgctgctgct tgaagtactt ctgggatttt ttcaccacca gagaaatcag agttacggaa
     7561 atcgggagaa ttaccacagc gatcagagtc atcagcggcg agatacgaag catcattacc
     7621 agtacgccga ccatagtcgc cacggaagtg atgatctggg tgatgctctg gttcaggctc
     7681 tgtcccagtg tgtccacgtc gttggtgatg cgggacaaca cttcgccgta ggtacggctt
     7741 tcaaaatatg ccattggcat gcggtcgatt ttttccgaaa tgtctttccg catgcggtag
     7801 cagactttct gtgtgacacc agtcatcagc cagccctgca caaagctgaa cagagtactc
     7861 agcagataca gtcccagcac aaacagcaaa atctgtccga tcttggtgaa atcaatgctg
     7921 ccagttccct gaatcttcgc cagcagtccg ttggaaagtt ccgtggtcgc cttacccaga
     7981 atgtccggtc cgatcacgct gaacacggtg gaggcaattg cacacagaat taccacggcc
     8041 actgcggcat gatattgctt catatatgca aacagtttgc caatagagcc tttaaagtcc
     8101 ttcgcctttt ctaccggcat ctgtccgcgt ccgcccatac gccgtctttc attcgccatc
     8161 tgtcatcgct ccttcctccg gtgtttcctc caagcccagt tccgacgggg aaagctggga
     8221 cttggcgatc tgctgatata cctcacagtt cttcagcagt tcctcatggg tgcccttgcc
     8281 gacaattttg ccttcttcca gaaccagaat ctggtcagca tggagaatgg tgctgattcg
     8341 ctgtgccacg atgatcatgg tgctgttctg cacctgttct cccagtgcct ttcgcagagc
     8401 cgcatcggtt ttcagatcca gtgcggagaa gctgtcgtca aagacgaaca ctttcgggtg
     8461 cttggcaatg gctctggcga tagtcagacg ctgtttctgt ccgccggaaa cattggtacc
     8521 gccctgtgca atggtactgt cgaaccggtc gggcttctct tggataaatt ccattgcctg
     8581 tgcaatttcc gccgcctgct gtacttctgc atcggaagcg tccggattgc cgaaccgcag
     8641 gttgctggca atggtaccgg agaacagcac gcccttttgc ggaacaaaac cgatctgctg
     8701 ccgcaattct tccagcggca ggtcacgcag ttccacgccg tccagcgtaa tgctgcctgc
     8761 tgtgacatcg taaagacggg gaatcaggtt caccagtgtg gacttgccgc agccggtgct
     8821 gccgatgatc gccgtggtct ggtttggctt tgccgtgaag caaaggtctg tcagagcgtt
     8881 ctcctccgca tgcggatagc ggaagcagac atgatcaaag gtcagcacgc ccttgctgtc
     8941 ctgcaaatgt ttcggctgtg ctgtttcctg tacagaggtc ttggtttgca gcacttcgtc
     9001 aatacgtttg gctgccacag aaccacgggg cagcatgacg gacatcatgg tcagcatcag
     9061 gaacgacatg acgatcatca tcgcatacgt aatgaatgca gtcatagagc cgacctgcat
     9121 agagccgccg tcaatgcggt gagaaccgac ccacacgatc agaatggtca ggctgtacat
     9181 gatcagcatc atcaccggca tcatgaaggt catgacacgg ttgacaaaca ggttggtctt
     9241 ggtgaggtcg cggttcgcct tgtcgaagcg ttcttcctcg gtcttttccc gtccgaacgc
     9301 ccgaatgacg gacagtccgg tcaggatctc acgggaaacc agattcacct tatccaccag
     9361 cacctgcatt ttctggaact tcggcatagc gatcgccatc agcacgccga cgattcccag
     9421 aattaccagc actgccagta caatgaccca gcccatgccg gctccggtgt gtaccacttt
     9481 cagaacgccg ccaatgccca gaatcggagc atacagcagc attcgcagga aaatcgccgt
     9541 aaccatctgc acctgctgaa tatcattggt gcttcgggta atcagtgaag cagtggaaaa
     9601 ctgatccatt tccgcactgg aaaactgcat gacacggctg aacaccttgc cgcggagatc
     9661 acgtccgaca ccggcaccca cgcgggaagc cacataacaa acacaaatcg ccgcagccat
     9721 catggcaaat gccatcgcca tcatcttgct gccggttcgc cagagatagc tggtctggat
     9781 cttgtctatg tccagccctg cgtctttgtc acactgcacc gcataggcaa cgcccataga
     9841 ctgtaccagc gaatccccca tagtgtccag catatcttcc agctgggaac gcatctgggt
     9901 gactgtatct tcattgagct gtccgctctg ctgcattgcc gcaaacacgg ggcggacatc
     9961 gacacaagtc actgtggacg agttgccgtc gctgtcctcc tctgtccgct gaaacggttg
    10021 cagttctacg cccatggcag caccgagttc ctccacggtc atctgctcca tctgagccgc
    10081 cgcgttggga tcggtctgct gctgtgccag catctcccga aagctgtcct cactcatcat
    10141 ggacatctga tagttcagca gcaccggtac cagcaggtcg ctgtccagtt cgtccagatc
    10201 tttgcccgac agactttcct ttctctgttc tccgtcatag gcattctgcc acttctgttt
    10261 ttcgctttcc gtcatgaaaa gctgggcgtt ttcgaattct tctgccgtga tctgttccgg
    10321 cagaatgtgt tccacgccgc tgttctggat tccggtgtca ataatatccg aagtgtactg
    10381 cggcagggac aggtcacaaa atgcctgtac caccaacagc accaagatca gcaacacgga
    10441 actccagtag ggtttcagat ttttgaaaag tttacccatt ggtcgctctc gctcctttca
    10501 tttcctccag ttcctctgag gccgccgcat agagccgctg aagcagttcc atcagctggt
    10561 cggcttcttc ctgtgtaaag cgtttctgaa tccccctgtg aaaatcctcc aacagctgat
    10621 tcgcccgctg cacctctgcc tcgccggcag ccgtcagtgt cacataggta ttccgccggt
    10681 cagtgcggca tacttcgcgg cagatataac cgtcgttttc tagcccacgc agcgtgcggg
    10741 aaactgccgg ctggggcacg tgcatcttct gtgtcaccat agacaccgtg ctgccctccg
    10801 gattggtttt ccgctgtccg gcaattacaa acatcaccat atactcctgt cgggacagcg
    10861 gcagcagcga aaacagattc agactgcgaa acgcatttat caccccgaaa aatgtttttg
    10921 catcacactt caagtgttcc acctcctatt cgtatttaat aacattgtta tgaaataact
    10981 ttgttctatt ataacgcatg ctatttattt tgtcaaggga attttgcagt tttcatctgc
    11041 ctgacacaaa aaagtgcatt ccgccaaagc acagcagaat gcacgaa
//
LOCUS       DAWBGR010000075        11041 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1733_length_11041_cov_4.041416, whole genome shotgun sequence.
ACCESSION   DAWBGR010000075 DAWBGR010000000
VERSION     DAWBGR010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11041)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11041)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11041
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1733_length_11041_cov_4.041416"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2689 a         3008 c         2618 g         2726 t
ORIGIN      
        1 catctgacga aaaatttgac gacgcatctg tcgcacaatc tttgtgcggt attgtcttta
       61 cttcgacgcc atttcgccca acgtccgaat catttccaga tcgtcctgtg tcacacgcat
      121 gttggtgcag agttctctgc cctcggcggt agtcaggctg atctcgatca cgctgccctc
      181 gttgattgcc ttagaagcag cggagaaaaa cagaggcact ttcgggtggt tctgccgaaa
      241 tttcgtcagc atggttttca gttgcaaaat cattttggga ttcatcataa gtcagttact
      301 ccttttctgg tatgcagcgg acagtgctcc gatacgtggg gaaacggcac cgtgcggaac
      361 tgcattcgca gtccgtccag caccagcagt tttcccgtca gaagcgacgg aattcccagc
      421 agataccgaa ttgcctccgc agcctgtatg gtgccgatga tgccggcagt gacaccaatt
      481 accggaatgg gtgccggtgt attgtcctcc ggaacctctc cgaagatgca gcggtaacac
      541 ggcccctttt ccggcacata tgtcatcacc tgtccggaaa agcctgtgat gccgccgtgc
      601 acaaagggct tttctgcgga aacacagaca tcgttgatca gaaatttgtt ctccagctgg
      661 tcggtcgcat caatgacaat gtcataatcc ggcagcacct gccgcaggtg tgcttcgtcc
      721 ataaactggg agtacgtctg cacatgcacc tgtgcgttca gtccgtggag agatttcgcg
      781 gcggaaacgg ctttgttctg ccccagacgg gattccttgt gtaggatttg ccgctggaga
      841 ttggacagct ccacggtatc gccgtctgca atgccaatgg ttcccacacc ggctgcggca
      901 agataatacg ccgccggact gcccagtccg cctgctccga tcaccagcac acggctgcgt
      961 ttcagagttt cctgaccagc ttcgcccagt tccggcatca tgatctggcg ggcataacgt
     1021 tcctgttcct gtgatgtcag catgatgctc ctttctatgt ttccttaaaa gtgtgcctgc
     1081 aagcccgtca ggtctttgat gacttctccg gcaaggatca gccccgccac cgacggcaca
     1141 aaggcacagc ttcccggaac cggtttgcct ttctctgtcc attccgtccg ttgcaacgct
     1201 gccggcagtt ccttggaata gaccactttc agcttgggga tcccccgctt tttcagttcc
     1261 cgccgcatca ctctcgccag cggacagaca gaagtctgca taatatctgc cacttcaaac
     1321 gccgtgggat ccagcttatt gccggcaccc atggcactga taatgggcgt tccggcggca
     1381 tatgcctgct ccaccagtcc cagctttccg gaaaccgtat cgatcgcgtc caccacatag
     1441 tcatactggg tgaagtcaaa gtcccctgcg gtttccggca ggtaaaattt ctgatgcttg
     1501 gtcacgcggc attccggata cagcagccgg atccgttctt cggcagcgtc cacctttgcc
     1561 atgccaatgg tggcacgggt ggcaatgatc tgccggttca gattcgatgt gcaaacagta
     1621 tcgttgtcga tcaagtccaa cgccccgaca ccgcttcgtg ccagagcctc cacggtatag
     1681 ccgccaacgc cgccaatgcc gaatacggct acccgtgcct gtgccagccg ttccatagcg
     1741 gctgtgccga aaagtaattc tgttctggaa aatgcattca tatatgtcta tcctgatact
     1801 tggtcaaaac ttggcgaact tcttctttat gtgccaaaaa cgttcccaca attttcggat
     1861 caaagtgtgt cccgctggat tcctccagaa tccggaacgc cttttccacc ggaaacgctt
     1921 ccttataaac acgcttgctg gtcagggcat caaacacatc gaccaccgcc atgattctgg
     1981 cacagagagg gatctccttg ccggaaagat gatacggata gccgctgccg tcccatttct
     2041 cgtggtggta caatgccatt tcctgtgcca ctttcaggta ggagtcactt tcaatatcgg
     2101 tcagcgtttt ctcaatgatc tccccgccgg aaagagtatg ggtcttaatg acttcaaatt
     2161 catcttctgt caatctgccc ggtttattca gcaccacgtc cgaaatcttg atctttccga
     2221 tgtcgtgcag cggtgccgcc ttgcacatgc ggtcctgcat ctccggtgtc agtatttttg
     2281 catactccgg catttccgcc agatatttca ccaaaatacg cagatattcg ctggttcgct
     2341 tgacatgctg accggtgaaa tcatctcggg attccaccat atcggcaaag ctgacgatga
     2401 tcttgttctg gatctcctga agccgccgat tagagttccg cagagattcc tgctgcacaa
     2461 acatccccac acagctgtcc agtgtcagca cgaactgatg cattctctgc accacgtaat
     2521 caaatgaatt ggcaaacacc accagatagc catacaccat ctgctgattg tgcacggcat
     2581 aaatgaccac agggtctgtt ctttcggtaa tgtagttcca gtccggcacc agctgccgct
     2641 gcgggatctt cttgacttcg gtgttttcct ccccgtaaaa aaaccggagt gatgtcactt
     2701 cttctgtaaa cggttcagtt tcataagtat gcccgttctg attcccgtgg aaaaaatctg
     2761 cattcacaca gagataagta tccgaacgaa tatagtaccg gagaatctca ttgacttttt
     2821 ccacatcctg ttcaaatgtc agctttgtca gcatattggt catggaacgg tcgtactggg
     2881 cactgttgtt catgcgggaa tagatcgaat tcacggcaag accgactgcc ttcatggaga
     2941 ttttccggca gccgcaggat tcggaaacgt ccagcgtgta cggaaaaaca tattcccgct
     3001 tcatttcttc tcctgcattg gcacgttcta tcagcttgga gagaaaactg ccgaaccggt
     3061 tcaggtttct gcgacaggtc gtcagccgcg ggaaattata ctgttcctgc acaatgccgt
     3121 cgaatccggt cacaatcaca tcttccggca cacgaatacc ccgttcctcc agaatttcac
     3181 acacagtgat tgccatggag tcgttgatgc aaacgatcgc ctgcggcagg ccctccagat
     3241 cagcaagaaa gcgttccctg acttcctgtg tgggaatgct gtagaaattc ccatagccga
     3301 accacttttc gtcaaacgga acgccgtgct ctttcagcac gtcccgaaaa atcatttccc
     3361 gtatttccga agcgggattg cccttaaagc cggcaatgca ggcgaacttg gtgacgtggt
     3421 gctgttcgat caggtgcttc accagatacc ggaatgcatc agcttcatca aacgaaatgc
     3481 ggatcccgcc ggaatagcgt tctgtcggtt ctatcagaaa taccggtttc cggtgtttct
     3541 gtgccgccgt cagaatgtcc tccacgatcg cacggcattt cagacactgg gtatagatca
     3601 aaaccacatc gatcaccgaa aaatccatca gctgaaagat gctggcttcc ccgcggtcaa
     3661 atgccgtatc ccgaaacaga tctgttgtcg tattgaacag caggcaccgc cagttctttt
     3721 ccaaaagcga ctgatacagc ggggctatca tgctctgtgt attttcgttt tgtatttctg
     3781 acacacaaag tgcagccacc ttgtatccat tgatcatatc cttttcctcc gccgaaaatt
     3841 cctcggacag ctcagcctac agaacctgta aactgtccct gttgtcattt ttccccattg
     3901 caaaaacaac cccgcaaaaa agcgacagaa aagccttgtg cggtgttgcc taaaatatct
     3961 ctttttattg tagcatattc caattctgct gtcaatctgt atttccggaa tgtccggtgt
     4021 gtctgtttat ttttttacaa acacgcaaaa aaagcaccgc attttgcagt gctttcattc
     4081 cgtgcagtgc tgttctcaca acagcagttt tcccagtgtt tccacgctgt cggtcaccca
     4141 gtctgcacca tattgttcca gttcgccggc ttccgcgtag ccgtaccgca ccccgatgca
     4201 gggcacgcgg cattcctttg ctccgaggac atcgtgcttc cggtcgccga tcatcaccgt
     4261 ctgcggcggc tcctgctccg gcagtcccag ccgacgcagt gccagacgaa tcatgtccgc
     4321 cttggtttcg cccgcccgat gaatgtcaca gccggccaca gccgtaaagt acttaaacag
     4381 gtcaaaatgc tccaggatcc ggagcgtata gacctccggt ttggaagtcg ccactgccag
     4441 cacatagccg tgttcccgca gcttttccag cagctccgga atcccgtcat acaccgcatt
     4501 ttccagcagc cctacagtag agaaccgttt ccggtactgt tccaccgcaa aggcagcacg
     4561 tgccggcggc atgccgcaga actcctgaaa ggaatccaac agcggcggtc caataaacgg
     4621 ccgcagcttt tccgcatccg gctccggtgt accgcattgt tccagtgcgt actgcacaca
     4681 gcggagaatc ccctctgcgg agtcggtcag tgtgccgtcc agatcaaaca gcagataccg
     4741 gaggtctttc ggtaacgctt tcatgctcag cctcccagtg cgatcatgcg gtcaatacag
     4801 cagcgtgcct tggcaatggt cttgctgtcc agctggatct ccgtgccctc gccgtctttc
     4861 agaatgtgat aaatgtccat cagcgtggtg gacttcatat tctcacagat cagcttcttt
     4921 gccagcggat agaaccgctt ttccggacaa gcattttgca gtgtttccgc aatgctgatc
     4981 tctgtgccga taatgaattc ctttgcgtcc gactgcatag cgtaattcac aatgccggaa
     5041 gttgaaccga tatagtccgc ctgctcacag actgccggac ggcactccgg atgcaccagc
     5101 atcaacgcat aggggtgtgc ttcttttgcc cgacgcacgt cctttgccgt cagtgccgca
     5161 tggatcgggc agccgccctg caacagcttg atcttctttt ccggcagctg ctgctggaca
     5221 aaggaaccca gattgcagtc cggaataaag aggatcttgt cattgctcag ctgcgaaacg
     5281 atcttgaccg cagaagaaga agtcacgcag acatcgcaaa tggttttcag ttctgcggtg
     5341 gtgttgatgt atgccacaac ggtatagtcc ggatactgca tctttacagc acggatcatc
     5401 tctttatcca tctgttctgc cataggacag cctgccgtgg ggttggacag gtatacgttt
     5461 ttttcaggag aaaggatctt cacagtttcc gccatgaacc gcacgccgca catgagcacg
     5521 ttcttctgcg gtgtatcttt tgccttcacg ctcagagcat aggaatctcc ggtaaagtca
     5581 gcaatttcca gaatatcgct ggtctgatag ctgtgtgcca gaatacaaaa gtcgcgttct
     5641 tttttcagcc gcaggatctc ctcttgaatc tcacgtattt tcataatctc aattcctttc
     5701 gggacagtgt acttcatgcc acaatttctc atcttcaata aaaaatatgt gatttatact
     5761 tgcatttgca aaaatagata aacttattct agtatagcac aacaaatgga gtttgtcaag
     5821 attttcacag gatatttaca taggaaaatg ctatgttttg ttcctgtaaa atatagaaga
     5881 aaactgagaa gaaaactgga ggaatttcag gcagtcctgc aaagaaacag aatccacatt
     5941 tttcgcttcg gaaatttgtg caaaaaaggt acttcacaaa atgcaaaaag tatgttataa
     6001 tgatattatg cattccttgt gcatttccaa cagattccca aagcgatttc atgcttcatt
     6061 ttaacaattt tactattcac agaaagaagg ctctgcccat gcaaaaagcg gcacgcatca
     6121 ttggtatatg tctttcccgc gtacaggaag aatacaggct gcaatgtatc aaagcattca
     6181 agcaacatgc ttctgcaaaa ggataccgcc tttttatatt caacgcaaaa tccgactttt
     6241 ttctgccgcc gaccccgaca gacttcggcg agatgactgt ataccagctg atcccctatg
     6301 atctgctgga cgcaatgatc ctctttccgg ataccattta cagagattcc attctgaatg
     6361 acattctgga gaactgccgc agccggcaga tcccagtact gacgatcgat aaggctctgg
     6421 agggctgcat caatttccgg ttcgactatg ccaactcgtt tgaaaaactc tgccgccatg
     6481 tggtggaagt ccaccacgcc agaacgctgt tcatgcttgc cggatttgca gaaaataatt
     6541 tttccaatga acggatcgcc gcatttcaga aagtcctgca agaatacggg ctgtcgcagg
     6601 agaactgccg tattgcatac ggggatttct gggaaaagcc tgcaatagaa gccatggaaa
     6661 agtggtttgt agaagataaa ctgccgatcc ctgatgcaat tatctgtgcc aacgacacca
     6721 tggcgattac agccagtcag tttctgcaaa acctcggcta tcggatacca gaggactgca
     6781 tcatcaccgg atttgacggt atctcacatg caagctatca cgtgccgcac ctcaccacct
     6841 gcacgcagga ctatgacacc atgggacggc tggtcatcga agcagtagaa aagctgtggc
     6901 tgggacagcc tgtagaacct tccaccgttg tggattttta cgtgcggttc tcccagtcct
     6961 gcggctgtga ggcagtcggc tcggtcaatg tcaataccat tttgcagaac gtgctgaatc
     7021 agctgaagct ttccaacgaa cggcaggagc tgacatgtca ggtacagtcc gctgtcacca
     7081 gcatgtcctc actgtcggaa ctgccggaga tcattctgga taaattcatc ttccacacca
     7141 tagtctttgc cctgaatgac gacctgttcc gtgctccgga attcggcgag ggaaaacaga
     7201 aaaaacaggc attcagtgaa aatgtcaacg tcctctgcca gcggtacttc tggaatcccg
     7261 aggagccctg tgtgacctcc cgaagccact gatcccagat ctgaacaggc tgctggaacg
     7321 ggaagatcct gtgatcttca gtgccctgca ttttctggat ctcactatgg gctactgtgc
     7381 attccagacg gacatctcct atgattccta cgaaaagatc aacagcttca tgaatgccat
     7441 gggttctgca ctggggatct tccattccca gatgcacacc aaatccatca atatgcagct
     7501 gaaaagcgtc aacagcgaac tggaaaagct atatgtgcac gaccatatga ccggacttct
     7561 gaaccgccgc ggtttctacc gccagttccg gcagcagctg acggagagca agggaaagga
     7621 catgagcgtg atcatcattt ctgccgatct ggacggactg aagcacatca acgataccta
     7681 cggacacacc gagggcgaca acgccatcag cacagtgggc aaagcattga tgaccagtgc
     7741 cattcaggga gaagtctgct ctcgttttgg cggagacgaa ttcaccgttg ccggtgtcat
     7801 tgcagacatg gacgacaact atttcgaaag cttccgtgag agattccgtg agtatctgcg
     7861 gcagtataat cagatttccc gcaaacacta tctggtggaa tccagtatcg gcttctgctt
     7921 tcagaaactg gaagacgaga ttgatctgga tcagatgatc aaaattgccg acgaccgcat
     7981 gtatgaggac aaagtgcagc gacgaaaagc ccgccgctga tctgacaaaa aggtggtgac
     8041 attttatgca tgaaaaagaa atcgtttctt ctgccctgct ctaccgcgat cagacagacc
     8101 tcaaaaattt tctgaaggaa caacgccgca cagctccgcc ggattgttca tggctggttc
     8161 tgatacatac ctgttgtcac agtcccgcca gtgccgtcgc gatcgcacag cacgtgaaag
     8221 aagttcttcc cgatgccgca gtcctcggca ccagtgcagc cgccgtcatt catcacggca
     8281 gcatctatac ggaccagtgc ctgctgcaca tcacccgatt ccgccggaca cgtccggaaa
     8341 ttttccggct ttccctggac ggcaaaacac cggaggagct ggcagaagaa gccgcagaga
     8401 acttccccgc agacagccgt gccctgtttg catttttcac agatcagtac atgcacatgc
     8461 agccgttcct gcaacatctg gaacagctgc ggcagcacat tcccgctgcc ggcggcatga
     8521 tcagtgccaa cacattcggt gcgttttcct ttgatgaaag cggggtttat cctcacaacg
     8581 ccgtgtttgc cgttctgtgc ggcaccacgc tacgcacatg gagcggtgta gttcaggggc
     8641 aggaagcatt cggcgaaaca tataccatta ccaaaaccga gcaggacagt attctggaag
     8701 tagaccacca gccggcatct caatggtttc agcagaagct ggaggagatc tgccacacta
     8761 agccctgtcc gatctgttcg gaactgctga tgcggtttcc gctggcatta cagcaggtgg
     8821 gtacacaaag ccagtttttg agctatagtg aaccgcaaaa ccgcatacag acctattcca
     8881 actggctgcc ggcaggcaca cgcttccgta tggcatatct cagccctctg actgctgtgg
     8941 cagagctgcg ggagtgctgt gcagaactga acaccacgcc ctgtcagacg atccttgcct
     9001 atccgggaac gctgcactac tctgccatga caaactgcac tgcatgggaa ctgcgggcac
     9061 ttcgcagcag ccgcatttgc ggagcatttc tgcacggaga gatcgccgcg gcagaagaac
     9121 acaatcaagt ctatgtggga gctgatacgc tgttcggtct gacagaatcg gaacacgact
     9181 atctgcccat tgaccagacc ctgctggacg atttacaggg actggatgcc gactggcaca
     9241 acatcgatca atatctggaa catgcacagc agcaagcaac gatttcctct gcttcccgac
     9301 agatcatgca gcaggagatc ctgcttcgca gcaatacatt tttagataag gaaacggagc
     9361 tggaaaatct cgtgaaattc aagttcgacg acaaaaaaca tcgatacaac aagatctgca
     9421 tgatctccat tgaaaagggg atcctgatct ccggtcgccg tggttccaag gtatggaacc
     9481 agctgatgcg ggaaaatatt cagcaggtga tccgtacgct gcacgacacg gagcttttct
     9541 tttactgcaa cgataaatgg tcgtttttct tcaccgccga gccggaatat ccggacgata
     9601 aatttctgga acaggcagaa atggcgttcc atgaatgcgg ctattacttc tgcaaggaat
     9661 tgaacatcac agcagtcaac gccttttatg ttgtcatcgg cgaaaccgat ctgctggaaa
     9721 agatacagct ttgcatgtct gccacggcag accacaatgc ccgcttttat ctgtacaaca
     9781 aggataatgc cgaaaccatg aagcaaatgg acgaaaagct gcgtatgacc aacatcatca
     9841 gcgatgccat tttgtatgac cgcattgtcc cctacttcca gcccattcgt gacaaccgca
     9901 cacaggagat caccaagtat gaagccctga tgcggctgtc cgacaaggat cataacatct
     9961 atgctccggg gcagtttctg gaaatcgcca aggactatca cctgtaccta cagctttccc
    10021 agctgatgat caaaaaggta ctggaactgt tccgcgaccg cacagaaagt gtgttcctga
    10081 acctgtccgc ctatgacatc agttccgaag ccagccgttc catgctgtat gaactgttga
    10141 gtaatctgcc gcaggaggca tgcggacgca tcacctttga aattctggaa tcggagaaaa
    10201 tccgcgattt caacgaaatg gtgaacttcc tgaacgagat ccgcaagttt ggcgtgaaaa
    10261 ttgccattga cgatttcgga gcaggcttct ccaatttcac ggcgattctc cgcgtcaatc
    10321 cggactttat caagatcgac ggcgacctca tcatcaactg cgataaggat ccgatgaagc
    10381 agctttgttt gcaggcaatc tctgacattg ccaagggcat tgatgcggaa ctgatcgcag
    10441 agcacgtgga aaacagcggc gaacagcaga cggtagaatc taccaatatc cagtatacac
    10501 aggggtacta tttctccaag cctttgcctt atcagaatct ggagcagaag aaaaaggact
    10561 gaacgcatat ttgactgccc ttcgtgtaac acattgtctt ttaaaatagg tgtaggcgaa
    10621 ctcttttctt tttttgcttc tttttttctt ttcagtaaag aagcggcggg tcgccgctga
    10681 ccctacgccc caatttcaac tgtgaaaagg gtagtgttac ggcggcagca gccgcctgta
    10741 tgccccgccg ggggagtttt gttgatgaga atctcttgta tgatcttcgg gtgatattgg
    10801 aatcttacag tacagggaaa atgcgaactc ttttcttttt ttgcttcttt ttttcttttc
    10861 agtaaagaaa gcggcgggtc gccgctgacc ttacgcccca atttcaactg tgaaaagggt
    10921 agtgttacgg cggcggcagc cgcctgtatg ccccaccggg ggattgcggc gggtcgccac
    10981 tgaccttacg ccccaatttc aactgtgaaa agggtagtgt tacggcggca gcagccgcct
    11041 g
//
LOCUS       DAWBGR010000076        10928 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1751_length_10928_cov_3.466385, whole genome shotgun sequence.
ACCESSION   DAWBGR010000076 DAWBGR010000000
VERSION     DAWBGR010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10928)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1751_length_10928_cov_3.466385"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7828..7837
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2859 a         2493 c         2514 g         3052 t
ORIGIN      
        1 aaaaatttga cgacgcatct gtcgcacaat ctttgtgcgg tattgcccta aaaaaattac
       61 aaaacgggat acgaccggta aatccagtcg tatcccgttt tcttagctat tgatttgctt
      121 cggtaaatac tccgtttttc ttgcgaagaa tccgttgatt tgcagaaccg cgaaatgcca
      181 gctgcaagga cttctgtccc tccacaaaag gtccgtctac cagcacatcg atttcccgca
      241 gcaacgcatc agtggcatcg caccgatagg ggctgtctgc cagcaggtct gtttccagaa
      301 cacagccggt atagcaccag atgtcttttt ctgtaccgta agcttttcgc actgcctgta
      361 ccaattccag cagtacaggc tggttctccg gctccattgg ctccccgcca atcagtgtca
      421 gccctcggat ataactgggt tccagcagcc gcagaagctg ttctgtcgtt gcttcagtgt
      481 acggtgtgcc ataatcgaag caccacgttt ccggattgaa gcagttcttg caggcatgcc
      541 gacagccgga aacgaataag gatacccgca cgccctcgcc attggcaatg tcgcaagact
      601 tgattgcacc ataatacata tgcattcgcc tcttttacag gtgcattaca cggtcgcgga
      661 tttcctgtgt gcgcccctga ttccagaact gggtaccgat gtaaccgcag gtgcggcggg
      721 caacgttcag tttatcctga tcgcggttgc cgcagttggg gcattcccat accagcttgc
      781 cgtcatcttc tacgatctgg atttcgccgt catagccgca aacctgacaa taatcgctct
      841 tggtgttcag ttctgcatac atgatatttt cgtaaatatg ctgcattacc gcaatcacag
      901 ccggaatgtt gttttgcatg ttgggtactt ctacatagct gatcgcaccg ccgggggaga
      961 gttcctggaa ctgtgcttca aaagacagct tgtcaaaagc attgatcggt tcggtaacat
     1021 gtacatgata gctgttggtg atgtagtttt tgtctgtgac gccctcaatc acgccaaagc
     1081 gttcctgcag gcaacgggca aacttatacg tggtagattc caaaggcgtg ccgtacagac
     1141 tgaaatcgat attggtttcc tttgcccatt tcttgcaggt atcgttcaga tgccgcatga
     1201 cctttagtgc aaatggcgtt gcagaggggt ctgtgtgtga ttttccggtc atataccgtg
     1261 tgcattcaca cagtccggca tagcccagcg acagggtaga ataaccgccg tacaaaagct
     1321 tgtcaatggt ttcgcctttt ttcagacgag ccagtgcacc atactgccac agaatcggag
     1381 ccacatcaga cggtgtgcct ttcaggcgtt catgacggca catcagggca cgatagcaca
     1441 gctgtaaccg ttcgtcgaag atttcccaga aacgcttttc atcgccgccg gaggagcatg
     1501 cgatgtccac cagattcagc gtgacaacgc cctgattaaa gcggccatag aacttgtact
     1561 tgccggcttc gtctttgtaa acggtcagga aagaacggca gcccatgcag gtatagcagt
     1621 tgccctcttt cagggatttc ataatttttt cggaaatata atccggaacc atgcgttttg
     1681 ctgtgcactc cgcagccagc tttgtgagat aaaaatactt ggaatcctcg cgaatattat
     1741 tttcttccag tacgtagatc agcttcggga atgccggcgt aatgtagaca cccttttcat
     1801 ttttcactcc ttgaattcgc tgccggagca tttcctcgat aatcaatgcc agatcatctc
     1861 tggtttgtcc ctccggtact tcattcaggt acatgaatac tgtgacaaac ggagcctgcc
     1921 cgttggtcgt catcagagtg ataacctgat actgaatcat ctgtacaccc tgaatgatct
     1981 cgttccggac acgcatttcg gcgatctggt tgatcgtttc atcatcggta tcttttccca
     2041 gacgttcaaa ttcgcggcga acatcacggc ggaacttctc acggctggtc tgcacaaatg
     2101 gtgccaagtg ggacagggta atactctgac caccgtattg agaacttgca atttgtgcaa
     2161 tgatctgtgt ggaaatgtta catgcggtag aaaaactgtg cggcttttcg atcatagttt
     2221 cgctgataac agttccgttc tgcagcatat cctccagatt gaccagacag caattgtgca
     2281 tgtgctgtgc gaaatagtct gcatcatgga aatgaataat accttcccga tgagcatctg
     2341 taatatcatg gggcagcaaa aagcgggtcg taatgtcctt gctgacttcg cccgccatgt
     2401 aatcacgctg tacactgttg acaatcggat tcttattgga attttcctgc ttgacttctt
     2461 cattttcgaa attcagcaga cttagaatct tttggtcagt tgtgttggac tgccgcagca
     2521 gtccccgttc atagcggtag gtgatataat tttttgcaac ggtgaatgca ccgtacttca
     2581 tgatctcaat ttccacccag tcctgaatgg tttcaacgtc cactgcacgg ttcatgccgg
     2641 cacatcgtgt ttcaatgtca ctggcaattt tggcaatggt ttccgaggaa agctgctccg
     2701 cttcaatgac agtgatattt gctttcccaa cagctgcgat gatcttcgca cggtcgaaga
     2761 catcttcctt gccgcttcgt ttgatgattt tcacaattat tcctccaatt ccagataata
     2821 atcaaagaac tcagctttat gaacacagaa aagtacttca aaaaggtaag tttttttgga
     2881 aatgctttga gcagtattgc acagagcatg tttgttgatt ttgtgcggta ttgcttttat
     2941 tgcaattttc tttttcatgg caaacagaaa gctgcatgtt acagcagcct ttcttcccaa
     3001 tcattgataa aaatccgttt caaaatacga tgttgaacag ctattattat atcgcgattc
     3061 ttccgatctg tcaagaaaat tttttatgta ttttccactc gaaacacaac aaattgtggt
     3121 tacgcaactt gcgcaccaca agatatagta tttttcaaga aacactttcg aaatagcagc
     3181 atacatcttg accgccgtgt tcacatgctt ttcgtgctgg aattttccgg aaggaataag
     3241 ccgcttacat gtaacatata aagttcaaaa ggttcaaatt tttcggaaat gccatgcaga
     3301 aaaatatcgt tttgattgtt gtgcgactgc aaaaaatgag ttacttgtat tataccaaag
     3361 agaaattgaa aaggcaagtg atttccataa aaactgaaaa gaacaacttt ttgtcacacg
     3421 aaatttcaga aacaagaact ttcggaaaga atactgacac gttttttgtg gaacacagct
     3481 ttttgtgtat tttttgcaat ttttcaaatc agtgttgact ttttgcagaa tgtgtgctat
     3541 aataaaacca caatacactg tggcatgcat cacaaattgt gcaatgttac aaataaagga
     3601 gttttgattt atgaagactt tgaagaaagt cgcagctgtt ctggctgcat cgatggcttt
     3661 gaacgcagtt gcatttactg gtgcattttc tgcatctgct gaagaagacg ttaccgtaat
     3721 cggtactgca tccctgaagg gccagatggg aacatatggc tactggggcg aagacaatac
     3781 tggaaattcg gaagatctta caaataagcc ggtgcagatc acaaacgaag acaagcagta
     3841 tactgcatct tgggagatca ccggtgatgg cactggatct atcgaattcc tgatgctgga
     3901 gtttgatacg aacgatgacc atttcattac aaaggatact tatccggatt tggcaatcac
     3961 aattgactct gtaaaggtag acggcaacga ggtcgattac aagatgtctg ataatgcagt
     4021 agactacaaa tattatgcta atgataccgg tacaacacgt gcatatctcc tgaatacgtg
     4081 gaatgcatct ggtcgaaaag taaccgatat ttctgcggaa actgctgtgg ctcagagcgt
     4141 tgaagttact ttcagcgttg ccaatgtata cgctgctctg ccgtatggtg accttaacga
     4201 agacggtatt gtagatacta tcgacctgct ggaactcgca ttggacgtgg cacagaaggg
     4261 tgctggtatg ggttcttctt tcgatgagcg taagaccaag ctggcagatc tggatggaaa
     4321 tgatactttg gattcaatgg atatttacta tctgttgctg catattgcac agagtggtgc
     4381 tggcattacc gttgatccgt tcccggtaac tgtaaagtaa tcctatcttt tctttgaaaa
     4441 gtaaaatcta atacaaaagg gggagttgta ttttgcaact ctcccttttt gtatgtgata
     4501 taaaccagag cagaaaaagc aggcacaata ggagagagta ctcgtcgtat tgtgcctgct
     4561 cttttaattc aaatttgtaa acttattctt gtaccagttg cttcatgtcg tacatgccag
     4621 ccggcttgcc gcagagatag accgcagcgt ttactgcacc tacagcgaag actgtttttg
     4681 aagctgcact gtgggaaagg gtaatcactt cgtcctgtcc tgcaaagatc acgtcatgtt
     4741 cgccgacaat tgtgccgcca cgaatggaat gcataccgat ttctttgctg tcacgcttca
     4801 tgcggcgtgc gtgacgatcg tatacataat gatactcatt gttcagggtg tcgttaatgg
     4861 cattggcaat cataattgct gtgccgctgg gagcatcgat cttctgattg tgatgcttct
     4921 ctacaatctc aatgtcaaac tgtccgccca gaacagtggc agctttcttt gctagttcca
     4981 ccagcaggtt aatgcctaat gacatgttga acgtaaagaa taccggaatc tgttgagatg
     5041 cactctggat tttctggatc tgtgcatcgt catagccagt ggttgccagc accagtgcaa
     5101 cattgttgac cagtgcgtat tccagcaatt cgtccagtgt ggacggattg gagaaatcaa
     5161 taatcacgtc cggtacttcc ggaagttctg ccggagttgc aacaatgggg aaatccgcat
     5221 actttgcagt gtacgaatcc acaccgccaa ttactgtgca atcgtcccgt ccgtcaatac
     5281 aggaagctac ggtgtgtccc attttgccgt ttgcaccgca aattacaatc tttgtcataa
     5341 tccttgcctc ttttctcgtc cgaatatctt tccttaggag agcataccgt atgcacgcag
     5401 agttgcctgc attgcagcct tgtgctcatc gctcatatcc aacagcggca ttctgcaggg
     5461 accagagggc atgccaagct ggttcatagc ttccttgacc ggaatcggat taacttccat
     5521 aaacagatca ttgatcagac ggagatactt cagctgcaat tcggaagcag ctgcgaagtt
     5581 gttttccaga caatactgag tcatctgatg tgtttccttc ggcagtacat tagacagtac
     5641 agaaataaca cccttgccgc ccaaggacat gatcggaacg atcatatcat cgttgccgct
     5701 gtagatgtcg atcttatcgc cgcactttgc agcaaccttt gcggtatagc caatgtttcc
     5761 gctggcttcc ttgattgctg caatgttctt gtgctctgcc agtgctgcaa cggtgctgac
     5821 atcaaaacct acgccggtac ggctgggaac attgtaaaga atgatcggca ggtctacgct
     5881 gtctgcaatg gcattgtaat gagccacaag acctctctgt gtggtcttgt tgtaatacgg
     5941 ggtaacgatc agcagaccgt ttgcacctag ttcctgagcc tcttgagaga gctcaatggc
     6001 atacttggtg tcattgcttc cggttccggc aatgaccgga atccggttgt gggtatgctc
     6061 tactgcaaaa cggatgcatt cccgatgctc cttatcgtcc agcgtagagg attcaccggt
     6121 tgtaccacag atgataatgg catctgtacc gttttcaatc tgaaaatcaa tgttctttgc
     6181 cagaccggca taatctacag aaccgtcctc cagcatcggt gtaataatgg ctacgccagc
     6241 accggtaaaa atggtgtttt tcataaaaat tacctcctga tggtaatcaa aatctcaata
     6301 agatgtgtgc tttacagaaa tgcattagtc catatagccc tttgctgcca gcagttctgc
     6361 cagcagaacg ccgccgcctg ctgcaccgcg gagagtgttg tgagaaaggc agacgaactt
     6421 ataatcgaac atagtatctt cccgcagacg acctacagac actgccatac cgccttcaat
     6481 gttacgatcc agctttgcct gcggacggtc atcttcctgg aaatagtgaa tgaactgctt
     6541 cggagcagac ggcagttcca gttcctgcgg tacgccctta aagtcttccc agatcttcag
     6601 gatttcttcc ttggacggct tctgctcaaa ggatacgaat acagcggcag tatgaccgtc
     6661 ggaaaccggt acacgcaggc actgtgtggt gatcagcggg ctgtctgcac tgacgatctg
     6721 tccgttttcg attttgcccc agaccttcag cggctccttt tcggactttt cttcttcacc
     6781 gccgatatac ggaacgatgt tgtcaagaat ttccggccag cgttcaaaag tctttccggc
     6841 accggaaatt gcctgatagg tgcaagccag aacattctga atgccatact tccgcagcgg
     6901 ggacagcgcc ggcacgtagc tctgaatcga gcagttcgac tttacagcga taaaaccacg
     6961 cttcgtacct agacgctttc tctggctctc gatgacctcg atgtgttccg gattcacttc
     7021 tggtacaacc atcggcacat ccggtgtgaa acggtgtgca gagttgttgg atacgacagg
     7081 gcattctgcc tttgcatagc gttcttccag tgcacggatc tcatctttct tcatgtctac
     7141 ggcacagaat acgaagtcca ccatgcctgc gatcttgtca acatcagcag ttgcatcaaa
     7201 cagaatcatc tgcttcatag attccggcat tggcttctgc attgcccagc ggttgccgac
     7261 agcttcttca taagtctttc cggcagaacg gggagaagcc gccagtgcag ccacatggaa
     7321 ccacggatgg ttttccaaaa gtgtcgcaaa acgctgtcca accatacctg ttgcaccgat
     7381 aataccgacc ttgtactgtt tcatttttgt cattcctcca aaaaaatctt aaaaatatga
     7441 aaaaaccggt tgaaaaccgg cggctcatgc gatataatag aatagaatga cgtacaccga
     7501 atggtatatc gccgatagca ctccatcata acgatgacag tcatatccct gtttggaaat
     7561 atgcccagaa gctgtgtcac cggtacagct tcttcggcac gaacgccttt ctgctccgat
     7621 catcgcggaa tggtaacctg tcggaacata ctcatcgcaa gtgcgcctct acctattcct
     7681 atcatatcat ttctatggga atttgtcaat aacatttttg cgaaaaagag gaaaagctca
     7741 tgaaaaaatc agatttttac tatgatttgc cggatgaatt gattgcacag atccctgttg
     7801 agccatgagt tcactccaga ttgatgcnnn nnnnnnnttt ttatgatctg ttagagattc
     7861 tgcaaccggg tgatctgctg gtaatgaatg attcccgcgt tcttccggca cgtctgtacg
     7921 gagagaaagt agataatggc acctttatcg aattcctgct gttggaacag aaaggggata
     7981 agctttggga aatcatttgc cgtcccggaa aaaaagcaaa agtcggtgta aagttctcat
     8041 ttggcggtgg cagattgatt ggtgaagtcg ttgaagtgaa accggacggc aatcgaattg
     8101 tgcagtttac ctgtgagggt aacttttata ctgcactgga agaagtggga cagatgccgc
     8161 ttccgccgta tattcacgaa aagttgaaag acaaagaacg ctatcagaca gtttactcca
     8221 aggaactcgg ctctgcggcg gctcctacag ccggactgca ctttacaaaa gacatgctga
     8281 aaaagctgaa agagaaaggc ataaatgaag cctatgtgac actgcatgta ggattgggaa
     8341 cgttccgtcc ggtaaaggaa gatgacgtat tgcagcatca gatgcatagt gagcactatc
     8401 atctccctca ggaaacggta gatatgatac gggaaaccaa ggcaaacggc ggtcgtgtca
     8461 ttgcagtggg cacaacttcc tgtcggacgc tggaagcagt tgctacggca cataacgggg
     8521 aactggtggc agaagacggc tatacggata ttttcattta ccccggttat tcatttaagg
     8581 tactggacgg tttgattaca aacttccact tgccggaaag cactttgatt atgctgattt
     8641 ccgcatttct gggatatgaa aagactatgc atgcctacga aatcgctgta caggaaaaat
     8701 atcgtttctt tagctttgga gatgccatgt gtatcttgcc agagaaaagc gaatgaaatc
     8761 aaactgatgt gcttctttgg tatcagtacg atatggcatg cggaatgcat ctaacaatac
     8821 aatgataaag cagaaaggaa tttacacgaa tgtttacact tttaagaacc gaaggacatg
     8881 cccgaagagg catctttgaa accgtacacg gaaccattca gacacccttc tttatgaatg
     8941 taggaactgc tgcggccatc aaaggcggca tatcgtccat tgacttggcg aatgaattga
     9001 aatgtcaggt agaactttgt aatacctatc acttgcattt gcgtccggga gataaaatcg
     9061 tccgtcaaat gggcggactg caccaattta tgaactggca caagccaatt ctcacagaca
     9121 gcggcggatt tcaagtgttc tctttggcaa agcttcgccg tatccgagag gaaggcgttt
     9181 acttccagtc ccatatcgac ggtaaggcga ttttcatggg tccggaagaa agcatgcaaa
     9241 ttcagtcgaa tcttggctct accattgcaa tggcatttga cgagtgtgta gagaatccgg
     9301 caccgttgga ttacgtgaag aattcctgtg ctagaacgac ccgttggttg attcgctgcc
     9361 aaaaggaaat gcagcggttg aatagtctgg aggggactat caattcgcat cagatgctgt
     9421 ttggcatcaa tcagggcggt acttacgatg cgattcgtgt atcgcatatg caggaaattg
     9481 cacagcttgg gctggatggt tatgccattg gcggtctggc ggtaggagaa cctacagaga
     9541 tcatgtatca catcattgag caggtggagc cacacatgcc tgttgataag ccgcgttacc
     9601 ttatgggtgt aggaacgcct tcaaacatca ttgagggcgt tgcacgcggc gtagacatgt
     9661 ttgattgtgt aatgcccagc cgaaacgcac ggcatggaac gatctttaca tgggacggta
     9721 ttctgcatat caccaatgct gcttatgaaa cggattccca gccgttagat ccgaaatgtg
     9781 actgtccggt atgccggaat catacacgtg cttatgtgcg gcatttgttt aaggcaaagg
     9841 aacagctggc tggccgtctg gcagtaatgc acaatctcta tttctataat acgctgctgg
     9901 aacggattcg tgatgcactg gacgcagggg agtttgcaca gttccgcaag gaatacagcg
     9961 aacggctggc tcagagaatc taaggaaaca ttgtataaaa agaaagcgga tactgcatga
    10021 cacagtatcc gctgtatgtt cagaaacaag ttgtaaaaaa caaaggagca gggaagaaag
    10081 ttttattcgt aaatcggctc cagaaccgca tatttgccgt ctgcacgctt gtagacaaca
    10141 ttgacttcac cggtattggc attggtgaac atgaagaacg agtgtcccag caaatccatt
    10201 tgcagaattg cttcatccag atccatgggg tgcattacaa acttcttgtg gcgaatgact
    10261 tcgtgatctg cttcgacaac cggttcttcc agcatgccgg taaatgcagc ttctttcaat
    10321 ttcttttcga cacgagtttt gttcttgcga atctggcgaa tgatcttatc gatggaagca
    10381 tccaaagcat cttccttatc tacagcagac tgttctgcac gataaatcag gttgttataa
    10441 gtaaccgtca attcgatgac gatcagattt ttgtgtgttg ccattgtgat ttttgcttct
    10501 gcttcactgc cgaaaaattt gtccagtttt gtactcagac gcttttcagc atactctgta
    10561 aagttttgcg ggatctgcat ttttttcgct gtgattgtaa ttttcatgat gaattcctcc
    10621 ttctacagtt cgctgcaaga taccgaagca ttttacgttc gaactgtttg tttctcatat
    10681 tataacatat ttctagtttg gtggcaagta cttttttatt ttttgtggaa tacatacaaa
    10741 aacaattctc tggtttcgtg aaaatagctg atatttcgac gaaacagcac ttcatgattg
    10801 gcaatttata caaaacaagg ggattggact tgcaactttt caaaaaatcg attatcatag
    10861 tcatagagtg tttcatctat ggcaataccg tacaaagatt gtgcgacaga tgcgtcgtca
    10921 aatttttc
//
LOCUS       DAWBGR010000077        10830 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1770_length_10830_cov_3.519350, whole genome shotgun sequence.
ACCESSION   DAWBGR010000077 DAWBGR010000000
VERSION     DAWBGR010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10830)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1770_length_10830_cov_3.519350"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2762 a         2599 c         2931 g         2538 t
ORIGIN      
        1 aaagtcgcca gacgctcttt gtgcggtatt gcctaaaatt gatcttattg aggggcatcg
       61 tgtcagcgtg atttgcaatg acggcagagt atatgagggg ttcggagctg ttccgtgcat
      121 aggggaagat gagaatggtg aagaaactga tgcggtattg ccttatgaaa tgatatggaa
      181 acgaaaatgg gaggtatcgc tatgaaacga tcggattttc ctgataccaa tgatgaagaa
      241 cataagagtt ctcgtttttt gccctgtgcg ttgcggtttc tctatatcga tgatgacacg
      301 ctgcgtcgat tacagtcgct ggagcttacc agttatgagg gatttccttt gcatctggat
      361 tgttgtactg tgcatggcga tagagcgaaa caggtttatt ggatcaatgt tcgtctggaa
      421 gattgggaac aaaatcaaaa atttcgcaat gcactggcag aagaatatgg aagaatggta
      481 gtgccgcaca ttcgaaatcc tcagattcgg gagtatgtgc ttgtcatgaa cgagaaaccg
      541 gtgtatatct ttttgcaata cagcggctgg gcagacgggt gttccgtgaa tatcctgaaa
      601 gtgatctcgg atcatactgc taaaaatgaa atcgagtatc tgaagcaagt catttcgaag
      661 tgtgcaatgc tgcaattttc cggcggaccg gagtattggt atcgggatga ctatgcggtt
      721 ttcataccga gccagaaatg aatggacttg caaagatttt ttcaaaaaag cttgacatat
      781 agatagaatt atgctataat gaaaacatca tcatttgcgg aaggagtgtc atatcatgca
      841 gacgaaagga acaaagcaga tttgcgaaaa ccggcaggca cggcatgaat atttcattct
      901 ggaacgctat gaagcaggaa tttctttgca ggggaacgag gtcaagtcta tccggcaggg
      961 gcatgtgaat ctgaaagatg cttggtgcga tatttctgac ggagaattgt ttatcaaggg
     1021 catgcatatc acaccctatg agaaagacgg actgttccgg acagacccta tgcgggtgcg
     1081 gaaattactg atgcatcgtc aggagatcaa ccgtatgttc ggcaaggtga agcaggacgg
     1141 tttgacattg gtgccgcttt cgctgtattt taaggattcc cgcgtgaaag tacaggtggg
     1201 actctgtaag ggtaaaaaac tgtatgataa gcggcagacg gctgcaaaac aagatgccca
     1261 gcggcaaatt cgccgtgcag taaaggaaca caaccagtaa agattggcag aatgggggcg
     1321 taacggtttc gacggggacg atgaagcatg ataagcgagc agagattgtc gcatctcttt
     1381 aaacagcggc acgtttaaaa ttaaacgcta aaaagaactt ttctgtaagc tttaacaaga
     1441 gcctgcaggt agctgcctaa gtcagctact gtcgcccgta ggagtaccac ggcctacgct
     1501 gcggcatcat tgcagtggtg aacgttccgg cggcctgttc acaccgcagg atcggatttg
     1561 aactaccttt cttagcagcc tgtttaccgg cggttttgaa agggaatcaa tgtagataaa
     1621 ctacgctcgt agaaagttgt gtggatttgt tttcggacac gagttcaatt ctcgtcgcct
     1681 ccaccacacg caatactgca cgaactctcc cgcagagcag cggttacctg ctccagcggg
     1741 ggagtttttg ttttatcggt cagattgacc agcaccgtga tctgctcctt gtacacgatg
     1801 acctgattca caagcagctg caggatcttc tccgtgccct ctgcctgtac ttccagcaga
     1861 cggtgtgcga agtactggaa atgctccagc ttcagctccg gtgcgtcggt ttgcagcagt
     1921 cgcacttccg tttccagagc cgctttctgc tgctccagct gctgcatggt cgtttgcaag
     1981 gcagccgatg taatgccgct cagaatggcg ttgacggcgt tctccagttt cttttccgtt
     2041 tcctgcaatc gccgctgtgc agctgtcaga tcggcgtttt cgtgggtgct ctgctggtac
     2101 agtgcatagg tggcatttgc aatctgctcg cacgcctccg gagtagtgta ctgtgcaatc
     2161 gcatccagca cagcgtgttc cagctctgca gcaggcagcc agccgcagca cttgttcggg
     2221 cagcagtagt aataataccg cttgtccgtt ttgctggttc cggctctgcc ggtgagccgt
     2281 ctgccgcaag tgccgcagat cgctttcccg gtcagcacat agtcatgggg actttgggtg
     2341 tggcggtgct tgtgtctgga ttccgtgaga cgctcctgca cgctccggaa cagctccggc
     2401 tcgatgatcg ggggacagtg ggcatcttct ccggcacact ggaacgtccc tgtgtatctc
     2461 tggttggcaa ggattcggga aatggtatca atgcggaact gtttgccgcg gctggtagta
     2521 tagccggcgt ggttcagatc gtcgcagatc tcagtgaatg tcctgccgct gtcatagccg
     2581 gtgaagatcc ggcgtaccag tgatgcacct gccgggtcga tctccatctg cttgtctgct
     2641 ccgatgcgat agcccagagc cacgttgccg ccggtagtct ttcccttgat ggcgttctca
     2701 cgcatgccac gcttgatctt ctgggaaagc tccgcgctgt agtactcgtt cattgcctcc
     2761 agcagtcctt ccagaatgat gccttccgga ctgtctgtga tatgctcttt ggcagagaga
     2821 acccgcacgc cgttggcttt cagcttgctt ttgtacatgg cactgtcata gcggtttctg
     2881 gcgaagcggt caagcttgta cacaatgacc gcctgaaagg tcttgtacct gctgtcggcg
     2941 atcattcgct gaaactccgg gcggttggca gtcgtgccgc tgatcgcacg gtcgatgtac
     3001 tctccgacga tgtggatgcc ctcagacgct gcaaacgcct tgcattcccg aagctgcccc
     3061 tcaatggact gctcggtctg ccggtcgcag ctgtaacggg cgtagatcac tgcattcatg
     3121 tgtaaatttc ctcctaagac ttgatttttc agaggaaata tgctataata gaccttgttg
     3181 tggaatggct attatcgcaa tagtttttcc tcttgccgct tcacggttgc tgccgtgggg
     3241 cggttttttt atatttgatg ttgccctgcc gattttggta gggcattttt gtatggttac
     3301 gattgcctac ggattgcatc tttcgcacgg tgtatatccc tgtgcaatca gatcttcccg
     3361 tgttccattg tactcgctgc ggtttgcgtc ggaaattctt ttcgcactgg agcagctggg
     3421 atagtgaaat tttccagtgt tggtattgat gacataattg cgtgtatttc ccgccggctg
     3481 ctctgccgga gtttcttcct gtgccggagc aggcgggtct gttggtgctt cggtgggcgg
     3541 ctctgtcgca gccattgtgg tttctactgc tgttgttgtg gtggttgtgg ccgttgtcgc
     3601 ctttgttgtg gatgtagttg cttttgtcgt tgtggtagtt tctgtggcag tcgtggtagt
     3661 gaccacagtt gtggttgttg tgacagtgct gtccttcttt tggctgctgg tggtgtggct
     3721 gccggccgtg gtgaacgcca gcagtccgca gaccagaaaa atgcctgccg tgatcgctgt
     3781 ggcacgtttg cggtatttcg gatccagttc gctgtccagt ttttcaaaga gccggttcac
     3841 tggaatgaaa atgaatgcgg caacggcaaa gcagagaaag ccgctgaaag tgtgggcgaa
     3901 gatcgcaatc acggcaagta ctgctgaaac agcgaaagcg atccagcgaa acgtcttatt
     3961 ctccatcatt gcttctacct ccgcagtaag ggcatacgcc gctgtttggc atctgtgcac
     4021 cgcagtaagc acacacccgt ccggacggtg catctgcggc agacagagca ggatgtttct
     4081 tgtacaccca gaaccagagt gccgccgaca ggatcagggc gacaccaaac caaccggtaa
     4141 aggtcagaaa gacaacgccg gcgactacgg cagtccaaag aattttggtg cgtttctggt
     4201 atctggggtg tgcccagatc aggattagtc ccaatggggg aaagaagatc gtgggcagag
     4261 caacgacacc gtcttgacga taccacggct cttttcctga ggaatgataa tttggcatga
     4321 tcgatcaggt cctttcttta tggaatttgg ttatgccgtc ttaatcatac cacagtttca
     4381 tagatttctc aatgattcga caaagactgt aatcgaattg ttaccagttt gtcgaatcct
     4441 gtcgaatggg agtatagaaa accgctctgt ggcttgtccg tcacagggcg tttttctata
     4501 tttccggaaa catagaattg gttagtcgtc aatcacatcg gctgtcccca gcacacgccc
     4561 gatgcagcgg ttttctgtcc cttgcaggga aatgggatca tagctgctgt tgtgggagat
     4621 caggcagccc ttacccaatt ctttgatata gccgcagccg tccacccaga acacgccgat
     4681 ctcgccgact tccacgctgg gggtctgctg tacgcagacg gtttcgccgt cgtggaacgt
     4741 ggtttccatg ctgtcgccct cgatctcaag caggaagtct gccttgcggc tgtcgtctgt
     4801 atccttaacg gtgacggttt cccatgcgtc agaatcgttc agatcgtagc cggttccggc
     4861 ggacaccttg tgcaggctgc gtttcatgac aagcagcttt tgcggagctg tgccgttctt
     4921 tcgctgcaat gcttttgcag tggcgtcatt gatgaaccgc accactgcct gccgtgcctc
     4981 gtggggcagc tccatgtact tgcggagcag ttcctcttcc agtgcggaaa aagacttctc
     5041 cgaaaataat cgctccagcg catctgtcgg tggctgggcg gtcagtctgc cgaggaggta
     5101 gtcggtggac acaccgtaga agtcggcgag ttttacaagt gtgtcgtagt ctggctttcg
     5161 atcgttgcgt tcgtaattgc gatatgaacc gccttttatt ccgattttag aacacagttc
     5221 ttctgaagta agaccttttt tcgttcttag tcgttttaat gtatcgccaa tcattttttc
     5281 acctcctctc tacaataata gaatatcaca aaaccgaaca tttgtcaata tctgtgtttg
     5341 actttcggga aaaagtatct tttttgcacg aaagcgaacc aattcttttg tgcaatgtta
     5401 caaaaacgaa caaaacgcat tgacaaatgt tcggatatgt gatattatag aatcacagca
     5461 agggacaaac ccgaacaaag ctgaatccga cggcaggcgt cagaaaggag gtgaaaccaa
     5521 ccatggcagg attaattgct ttacttgcta ttgttattac aattttaggc atgattttga
     5581 actcgaaata aaattgtatt atcacagata caacggcata tgaagtttgt taaaatcaat
     5641 accgacaact tttgttttgt ttccacatac aatcttgatt ttgcatttcg tatttttcaa
     5701 atcgtcaatt ttaatatagc aatctggtac gagaaagcat ccgctgattc cctgatgcgg
     5761 tggaaggtgc tgcggcaggg ataaagaata atattcccct gttgttttat gattcgtttc
     5821 aatagaaaac aggttttctg atgcagcaga aatatacttt tcccgttctc cgaaaatgaa
     5881 gaaagcttgt gtaattgtgc atggcgtttc agacagattt gtgattgcaa tctgtatcgc
     5941 aagattctta tcgcatccat gattaaacaa aaaatactta tatgcaaatg gcgttatttt
     6001 cagccggaac cgcttcgcaa aaaaccggaa cacatatgga ataatggaaa aaacaagagc
     6061 cgcaaatgac attagcaatt cgtatccggg atgtttatca aaataatcca caatattttt
     6121 gaaaaattcc atttcattca cccccctccc ttgccctatt ataacataat tcggcaaggg
     6181 atacaacaga aaggaatgtt ttttatgaac gaacccattc aaattcacta tgacaacgca
     6241 gaccgcccca cggtatcggg tcgagagctg catgaagtgc ttggcgtaaa tacaaagtac
     6301 gctgattggt tcaaacgtat gtgtgagtac ggatttcacg aagatgttga ttattcttgc
     6361 ttctcaaatt tgggaagcga aaatcagcac ggtggacaga acaaaattga ccaccagctg
     6421 accatcccca tggcaaaaga aatctgtatg ctccagcgga gcgaaaaggg caagcagttt
     6481 cggcagtact tcattgcggt ggaggaacag tggaacagtc cggacgccat catggcaaga
     6541 gccttgcaac tgtccaatgc caagctgaaa gagatgcaaa tcacggtttc cacactcact
     6601 gttgaaaacc agattatgaa gccgaaagca gactattttg acgatttggt tgaccggaat
     6661 ctgctgaccg gcattcggga aaccgccaaa gagttcggca tcaagcaaaa ggattttgtc
     6721 aatttcctga tggacaagaa gtacttgtac cgtgacaaga aaggaaagct cacgccgtac
     6781 gccaagccgc ttgcagacgg attgtttgag ctgaaagaat ccaaaaacga taccaccgca
     6841 tggtctggca cgcaaaccat gattacaccg aaaggtcggg aaacgttccg gcttctgatg
     6901 acaggtatct gaaaatcaag aaaaggaggt gaatcatatg aacaatacca taacacaaca
     6961 ggtggataag acggacagag agcagtatct ggaagcctgc cggaactatg cggcggcaac
     7021 gtcgcccatg gacggtctgg cactggcaca gctggcgatc gctgcgatct ttgccggaat
     7081 gcagatcggc gagcagaatg ctgcacgcac acagaaagga gcataacatg gcaaaaacca
     7141 acctgaaaga gatgccggtg gaagttcgtt cttccagcaa agaaccgcag aacgacttct
     7201 tcgcagcatt tctggagtac gtgaagcagc atgccgatga agcgatcgct gcggtagagg
     7261 cacagaaaaa tgccgcaaag taaatcaaga acgtgaaagg aagtatcatc atggaagaaa
     7321 agaagaaaca cattgagatc cacatcaaga tggacgagaa cgagcagatc accccgagtt
     7381 caaagataag caacgccaag gcaaagtgtg atgaaaatac gcagaaagag gtgatagaaa
     7441 atgaaagtac tggtagcatg cgaagaatcc cagcgagtgt gcacagaatt tcgtagactt
     7501 ggacacgagg cgtacagctg cgacgttcag gagtgctccg gcgggcaccc agagtggcat
     7561 gtacaaggtg atgtactgcc gtacattgac ggtagatgta tcgtcacaac aatagacggt
     7621 gttgcacatc agattgatgg tacatgggat ttgttgattg cacatccgcc ctgcacatac
     7681 ctcagcaacg ccggagcacg tcacctttgg aaaggtcacg tgctcaaccg agagcgatac
     7741 gcaaagggac ttgatgcaaa aaattttttt ctgaaatttt tgaatgccga ctgccccaaa
     7801 atttgcgttg aaaatcctgt cccaagcaaa gtatatgagc tgccgcgata tacgcagatt
     7861 atacagccgt atgaattcgg gcatccgtac agcaaaagga catgcctttg gctgaaaaat
     7921 cttccgaagc tggtaccaac cgaaactgtc gagactatcg gtactttttg cccatccggt
     7981 tcttacagca aaaaacacga cagcaagcat cgcggcgttt ttacaaaaga ccgtgcaaaa
     8041 aacaggagca aaacattccc cggaatcgcc agagccatgg cagagcaatg gggaaaagaa
     8101 aacccccgca ccggcggcaa ccggtaacgg gggcatggaa aataattaac cacaactatc
     8161 atatcacact taggaggaaa tgtcaaatga caaagcgtga agcactggaa agagcaatcg
     8221 aaagcattcg ccacgacgac acaatggacg caaaaacatg gagaagcgtc atgaactggc
     8281 ttttggcagt acagggattg gctctgtgtg aggaaatcaa ggcggcaaac gaaaacgaag
     8341 atgacacaaa cgccgcagca gaggaggcat gccgccgtgg ttaaaaccat caccgaacgc
     8401 atcagcgact gcgtaggctg tccgccggaa ataggctgca tggggcaagc ctgcccgtat
     8461 cacccgcacg atgccgaacg ggaagtgctg gtgtgcgacg aatgcgaaca ggaaacagag
     8521 gaactcgtct gcctgccgtg ctatgacagc gacctgtggc tgtgcgagga ctgcatggac
     8581 acactggtgg acggtctgct gctgcggaag aaaggagagg ctctgacatg accaatcggg
     8641 aatatcacgc agaccccgcg atctccaagt ctgatctgga tcagatacac cgttcgccgc
     8701 tgcactggca gtaccgcaaa gcccacccgc cggagcagac accggcactg ctggtcggct
     8761 cggctgtcca caagatgata ctggagccgt ccggatttgc agacgaattc gtcgtcgcac
     8821 cggaaaccga ccgccgcacc agagccggca gggaagcgta tcaggcgttt caggaatccg
     8881 ccgccggaaa gacggtcatc acgccggaac tataccggct ctgcaccgac atggcggagg
     8941 cgttgcacca ctgccagact gcaagacagc tgctcaccgg cggcagggcg gaacagtcct
     9001 ttttctggga cgatgcccgc accggaatcc gctgcaaatg ccgtcccgac tatctgcgtg
     9061 acgggttctg cgtggactac aagaccacac aagatgcctc tccggaggcg tttcagaagg
     9121 cggcataccg gtaccgctat cctgtgcagg cgtactggta tctgcacgga ctggggaaaa
     9181 acggtctgtc cggactggac ttcgtgttta tcgcacagga gaaagaaccg ccgtatgcgg
     9241 ccgccgtgta ctatgcggac gagttgttcc tgcaactggg acagcaggag gcggctgcgg
     9301 acttggaaac actggcgaag tgcagaagca ccggcatcta cagcggctat ccggaggaga
     9361 ttttgccgct gacaccgccg aaattcgccc tcagagatct gatgtaaagg agtatcacac
     9421 atggaaaatc atgcattggc aagcaacccg tttgccgcac cgaaaaagca cgaccacacc
     9481 aatcagggca ccgtggagat cgaatccagc aaggcggtgg cagaggcaca gggcaagctg
     9541 gtgatcgcaa agcggttccc gcgggacgag atcaccgcct atgaaaagct gatgaccgct
     9601 tgttcccgcc ggaactttgc ggaaaaggcg acctacagct atccccgcag cggcagcacc
     9661 atcagcggtc cgtccattcg tctggcggag gaagcggcac ggtgctgggg aaacgtggac
     9721 ttcggcatca aggaactgtc ccagaaagac ggggaatcgg agatgctggc gtactgctgg
     9781 gacatggaaa ccaacgtcat gtccagccag cagtttgtgg tgcatcacac ccgcgacacc
     9841 aagaacggca tccagaaact gacggcacag cgggacattt acgagaacaa cgccaacatg
     9901 gcagggcggc ggctgcgtgc aaggattctg gcaattctgc cgccggacat catcgaagcc
     9961 gctgtggaac agtgccgcaa gacgcttgcc ggaaacgcag acgtgccgct ggaagaccgt
    10021 atccagaaca tggtggcggc attcaaaaag ctgagtgtcc gtcgggagat gctggaaaag
    10081 cgtatggggc atgacgtgtc ccgcatgacc atggaagaac tggtggagtg catcggcatc
    10141 tacaactcta tcaaggacgg cagcacccga ccggcagact ggtttgatgc tgtcgccgac
    10201 accggcaaga ccgatgccgt caacgagaaa ctgaaagggg aacagagcca tgcagacagc
    10261 gtttaaggac ggcagcgtta tggtgtgcgg cacactgccg aaagatgccg tgtacaagac
    10321 cgtggggcag aacagcagca gtctgaccac gttcggggta aaggtaggcg aaaagccgtc
    10381 gccggacgtg aaccggaaag ctgatgccgt gtggtgcaac tgtgagtgct ggcacagcgt
    10441 tgccagagcc gcacagcatc tccggaaagg ggacaccgtg ctgtgcgtgg gaaagatcaa
    10501 ggtggagcag tggaacggca aagacctgaa aaagctggtg tgtgagttcg tgatgcccat
    10561 gccccagaat ccgccccagc cggcaggctc tccgccgccg caggcagccg tgggaaatct
    10621 ctcggactat gaggaacttc tctccgacgg cgatccgttc tgaccagaaa tgtaaattta
    10681 taccgtatat cctgtaaatt ctcattcata gttgcacgtc ggcgtgcaac tttccgcctc
    10741 tgagagccta tttatggagg gacattgcag ccgcctctga aaaggtgctt gaatgaaaaa
    10801 tagcctcccc tttaggggag gggaaccgcc
//
LOCUS       DAWBGR010000078        10493 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1832_length_10493_cov_3.961487, whole genome shotgun sequence.
ACCESSION   DAWBGR010000078 DAWBGR010000000
VERSION     DAWBGR010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10493)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10493
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1832_length_10493_cov_3.961487"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2997..3006
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8481..8490
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2396 a         2981 c         2667 g         2429 t
ORIGIN      
        1 acgcatctgt cgcacaatct ttgtgcgata ttgcctaaag caacttttcc cattttttct
       61 gttgtctgca taaaaaaatt cctccggtgc aaactagcac tggaggtggt tttgatgaaa
      121 acccagacaa aacagaaatc cagattccgc ggattttaca cagccctttt cctgagcctg
      181 ctgatgatcg gtgccgcctg tgtctttgcg taccgccaga ccagtcagac actggagcag
      241 aacctcaatt ccctgaacga acagatggaa ctgaccgaat ccgaaacgga accctacgaa
      301 gcccctgttg tcggcgtgca gactgacgtg gcgaagcccg cagaaaccac tgccccgccg
      361 gcagccacca ctgcaccggc aacagaaaca gcagccaccg cagcagaaac agttccggaa
      421 acagttccgc cggaagaagc accggtacac cagctgaccc cgccgctggc agactttgcg
      481 atcctgaacc cgttcagtga cggcgaactc atcaagtccg aaaccaccgg tacatggcag
      541 acacacaacg gcgtagacct cagctgtgcc gccggtgcag acgttttcgc catagatacc
      601 ggtactgtca gcaaagtgtg cagcgatgcc ctgtggggct ataccgtgac cattgaccac
      661 gacaacggcg tgaccagccg gtactgcggt ctggacggct cgctggaagt gcgggagggc
      721 gacacggtac agaccggaca gaaaatcggt gtgaccggtg ccagtccgga cttggagagt
      781 gccctggagc cgcacctgca tctggaagtg cagaaaagcg gcgtgtatgt agatcccacg
      841 gcatatttcg gctgattacg gcacacaaaa aacgggacgc tgtttccacc gtcccgtaca
      901 tattattccg cctgctctgc atgttcttcc ggcagatacc agtaggagtg aaagccatgg
      961 agatacgttc tcgctccctt gggcatctgt gtcagggcat tgatgatatt gtaatagtcc
     1021 ccgatgccca tcggaataga agtcgctccc agtataccca gaaatcccag ctgcggaaaa
     1081 atcaggtaca gcaggaacgg cacaaatccg aatatcaggt tcggcatcag gctcatcaca
     1141 ataaaatggc tcttgctcat atcctccgtt gcaaccacaa acagcatacc gtttttcaga
     1201 ttggtgtaga tatacgcctc tttccggaag cacatggcgt ggataaactc atggggaatc
     1261 aggcaggcaa ttgccgccag aattcccgcc cagtatatcc aaaagtgttc cagtatcaca
     1321 tcaaagccgc cgcggagcag cacaatggca tatgtcacga tcataagcgg cacagaaagc
     1381 aaattcaaaa ccaaccccaa ttttttgatg tcgtcaattt cctggaattt caccgcattg
     1441 ggtttgtgct ctccgtgagg caaactctcc tcgttgccgt caaatttgcc tttccagatg
     1501 attctcataa gattttctct ctccctatca attgtaattc cggaactgct ccggcacaga
     1561 gatgtcgaac agttcccgaa tcttctccat gctccacacc tgataggacg gacctgtact
     1621 ggacgaacaa tagggcagtc catgctgggc agccagttca tccgtataca tgctgtaagg
     1681 atacagccgc agattggatt cccgcccgtc gtcaaactgg gtaatcaccg gcgaaacctt
     1741 gacatcttca atggtcacag agccgtcctc tttcgctaca atgttgaagt ctgccagttc
     1801 cccgatcagg ttcatgttgt ccgtctgggc agaaatgaaa ttccccagcg aataaaacac
     1861 aaagccctgc gtaccgtccg gacgggtcag atattccatt gtctgtgcca catgcgggtg
     1921 ggtgccgata atcacatcgc cgccccagtc aatgattttc tgtgccagtg cctttacgcc
     1981 gtcggtgacg gtataggtat cctccacgcc ccagtgacag gtcaccacta ccacgtccgc
     2041 aatttccttg gctgcggcga tctgctgctg gataatgtcc tcctgtgagg tgtacacgat
     2101 ctccagttca gagttgtccg gcagcgaata gccattggta ttctcggtgt agctaagaaa
     2161 tgccactgtt tttccgttga attccttggt gcggatatgc tgctgcatgt ccacatagtc
     2221 ccgatagact ccgcatgcca gaatgtccgg atactgctgc atcttctgat cccagtagtt
     2281 caggcagccg ttcagtccgc tttcaccctt gtccagcagg tggttgttgc acatggtgat
     2341 aacattgaag cccatgccca ccacggcatc ccccagttcc accggcgaat tgaagttgaa
     2401 gttggagccg gacacctcga agccgtcgcc gcagatcagc gtttcctgat tgatgatatt
     2461 caggtcaccg ttgattagcg gcttgatgtt gtcatagcaa taatcgaaat tataacttgt
     2521 cccgtcgttg gagtgtgcct gtgcactctg atacacgcac tgctgtacca gattgtcccc
     2581 caccgcgacc acgtgaatgg aagtgtcgcc gccggcttct gcggtggaat ctgcggtgct
     2641 gtcgccggac ggcgtttcac cgctgtctgc ggagctgtcc gaaaccggtg cgtttgccgg
     2701 aactgccgca ctgtcgttga tctcgttggt cttggagcag ccagccgccg cacaaaggca
     2761 ggcgataccg gtgagcactg ccgccagcct tttcccgtat atccaaatgt gttgtttcat
     2821 aaaaacgtcc tttcttcgac aaaatattca cttcattata gcacatctcc ggaaaaaaag
     2881 caatccattt tgcatctttt tttgagaaag cggcatactg aaaacagaac ggcagtcctg
     2941 ccgtgaatcc attcgggagg ttgccctatg caacaaaacc acacagagat tttattnnnn
     3001 nnnnnngatc cgcaccattg cagagggttc gtcagacctg ctggtcaacg aatttgtcct
     3061 cagcgggctg cctgccgccc tgctctgctg tgagggcatg ctgtctacgt ccaccatgac
     3121 ggaactgatc ctcaagccca ttaccgcgat ccggctggaa tgccccactg cggcaaatgt
     3181 ttttgccttt atccgaaatc accagctgta ttctatcgac cgtgcagagg caaacaccta
     3241 tgctgacctg ttccggtttc tgaactccgg ctttgccgtg ctggtgctgg acggagccga
     3301 ttctgcactg gtattcggga tacagggcta cgacaagcgt tccgtcagcg agccctccgg
     3361 cgaggacaac gtcatgggag caaaggacgg cttcattgaa gttgtccgcg tgaacatgtc
     3421 cctgcttcgc cgccgcatga aaaccccgca gctgaaactg gaactgtttg tcaaaggttc
     3481 caaaagccag accgacctct gtctttgcta catggcagac cgcgtgccca aatccctgat
     3541 cgcacagatc aaggcacggc tggacgaaat ggaactggag tccatactct cctccggcta
     3601 cctccagccc tttctggaga accgccgcgg ctgtctgttc gacactgtgg gcactaccca
     3661 gcgaccggac gtactctgtg ccaagctgct ggagggacgc gtcgggctgc tgatcgacgg
     3721 cacgccgttc gcactggtga ttcccaagct gttctgcgaa agcttccaga ctatggacga
     3781 ctactgctat cgtcctgcct atgccacgct ggttcgctgg atcaagtacg ccgcctttct
     3841 ggtggcagtg ctgctgccgg cgttgtatgt ggcgatcgcc ctgcaccacc ccgaactgct
     3901 gaaccgcacc ctgctgctga ttctggcaga tgccgaggaa aacgccccca tttccctgac
     3961 tgccgaagcg atcggcgtgc tgctggtcta cgagatcata cgggaagccg gtctgcggct
     4021 gcccaaagcc gtaggcggtg ccgtgagcat tgtcgccgga ctgattattg gggacgcggc
     4081 agttgcctcc ggactgatca gcactcccat gctcaccgtc accgccattg ccgtcattgc
     4141 cggtttcgtc gtacccgatc tggcacagag catcaccctg ctgcggctgg cgtttctgct
     4201 gattgcagga ttctgggggc tattcggcat cagtctgctg ggggcagccg tgctgttcaa
     4261 tctctgtgcc ggcgaaagct tcggcttccc gatcacagca cccattgccc cgctgtatca
     4321 aaagggcttt cgggataccc tgacgcgggt cagcttccgc aagatgcagt ccggcagttt
     4381 caccgtggag gaataccatg aataaactcc gttccaacca gatctttgca attctgctgg
     4441 gcagtgccgc ctttcccctg ctctgccgca ccgagccgtt caccattgag ggcatcttcg
     4501 gggctgccat tgccgccgca gtacagctgc tgctctgtat tcccatactg ctgctctatc
     4561 ggacagggtt ttgttttggc gaatgtgccc gcagacaccg cattctgccc ctgtgcttta
     4621 ttgtctatct gctgtttcgc ggcgggactt cctttgtgca gttacagcag acctcacagg
     4681 aaatttccct gcccttttcg gaaaagtttc tggcggcggc actgatcgca ctggtatgtc
     4741 tgtacaccgt atcgctgggg atccagacca ttgcccgcag cagcaccatg attctgggaa
     4801 tcctgctgat tacgctggcg atcatgtgta ttggtgccat tccgcaggca gaaccgcaga
     4861 atctggcact ttctcccaac gataccattt ggaaaggctt tttccgcagc atgaccaccg
     4921 cagacgaact gccgctgctg tttctgctgc tggacttttc cgagaagaaa acctaccgca
     4981 gcattgtgcc ggtatgggtg ggaaagttcc tgctgggcag ctatctttcc gtgctgggca
     5041 tggcggtact gggcagtcgc atggcaaaag ccagccaccc gtttttcgcc attgtttccg
     5101 tttcccagcc cctgagcacc cagcgtgccg atgcccttta tatcttggta ttcgtcatgc
     5161 tctgcgtgct gcggatcacg ctgttcgccg tactggcggc acatctgtta aggctgtgct
     5221 ttccgaagct gcgttacacc agtaccctct gtctgctgtc tatgctgggc attgcggggg
     5281 cagcttcaaa aatttctgtt tccggtatct gggagttctg atccggttct gctgctggca
     5341 acgcccgaaa ggaggaactg tttcctatga aatataccat tgcatttccg gctggattga
     5401 ctgcggcagt cctgctgctc tgcaccggct gtgccgccac ccaagtgcgg gacagggcat
     5461 atctacaggc gatcgaactg cgatctcctg acacgccgac ggtactgctg cacgactttc
     5521 acgaatcaaa agcgattgcc tacggcaagg acagttccgt ttctcaggca gttgccaacg
     5581 ccgccgttcc ggtaggaaag acactttttc tggggcatct ggaactgatc gcctatgaaa
     5641 atcccgcatt tggagaacag ctggacgacc tgatgcagca gtaccgcctg tcccccgcct
     5701 gcaaggtggc atgttccgcc gacggcatca cccttgcaca ggaggacacc accaaaaccg
     5761 tagaacagct gcggcaggcg gaacacgccg gacagctgcc ggaaaccgac ctgttcacca
     5821 ttctgcggga atgggacacg gcttccggca cggcactggt tcccctgatg acagaggacg
     5881 gattttccgc cgccattgca accaaaacga acctctccgc tgccctttct ccagatgcgg
     5941 tttccggact ctgctggctc cgcggggaca attatccgga acagttctcc acagaaagcg
     6001 gcagctacac tgtttcgtct gccaaaacac agctttccgc ggaaaaactg ccggacggat
     6061 tccacgtcac cgccgccatt ttcctagaag gcgagggcga ttttgaagcg gcatcacagg
     6121 agatcacaag gctgtgtcag gcggcatttc gggaaaccgt ttccgaatat cacgcagacg
     6181 tgttcgatgt ggagccatgc ctctggagcc agtgctacaa ggatatggaa caaagcgact
     6241 ggaaaactgc cgcagggcag atgcactttt ccgtacaggt caacccgcac aagaacatgc
     6301 tctgaaatca aaaaggacgc ttccgccgaa acggaaacgt cctcttttta tgcaatcacg
     6361 caattatttt ttatgccaga aaaaatgttc cttactttgc agccttcttg atgcggtctt
     6421 ccagcttatc cagctgttcg tacagagcag ccggaatgtt gccgccctta gccttgatgt
     6481 cttcatcata gtatctacgc atctcggtga tctcagattc ccacttatcc atatccagct
     6541 tcagcaggtc tgcgatgtca tcggaagtga actgcttgcc ctcgtaaacc agaccgtcaa
     6601 cgttgatgtc ctcagccttc ggctcatagc cgatcggagt ttcaacagcg tctacagtgc
     6661 cttcgcaacg cttgatgatc cagtccagaa cacgcatgtt gtcgccgaag cccggccaca
     6721 ggaagttgcc ttcatcgtcg gtcttgaacc agtttacgtt gaagatcttc ggagccttgt
     6781 cgcccagctt ttcgcccatt tccagccagt gtgcccagta gtcacctacg ttgtaaccga
     6841 tgaacggctt catagccatc gggtcgtgac gcagaacgcc gactgcaccg gtagcagctg
     6901 cggttgtttc agaagaaaca gcagaaccca tataggtgcc gtgtgtccag tcgaaggact
     6961 gatataccag cggagtggta gatgcacgac gaccgccgaa gatgattgcg gagatcggta
     7021 caccttccgg attgttgaat tccggagaga ggcacgggca gttctttgcc ggagaggtga
     7081 atctggagtt cgggtgagcc agcttgccgc cttcagcgat gaattccgga ccatttacct
     7141 tcttgccagt ccactcagtt gcgtcaaccg gcggattctt atccagcttt tcccaccaaa
     7201 cagtgttgtc tgcattgttc agagcaacgt tggtgaagat cgtgtcagac ttggtgcaag
     7261 ccagagcgtt cgggttggag tggtcattgg tacccggagc aacaccgaag aaaccattct
     7321 ccggattgat tgcatacagt ctgccgtcct tgcccggctt catccaggcg atgtcgtcgc
     7381 cgactgtgaa taccttatag ccattcttct tataaccctc cggcgggatc agcatagcca
     7441 gattggtctt accgcaagca gacgggaaag cagcagtgat atacttggtt tcgccgttcg
     7501 gcttctgaac gcccaggatc agcatgtgtt cagccatcca gccttcgttc ttgccctgga
     7561 aggaagcgat acgcagagcg aagcacttct tgcccagcag aacgttgccg ccgtaagcgg
     7621 agttgatgga ccagatcgtg ttttcttccg ggaactgtac gatataacgc ttttccggat
     7681 caacatctgc cttggagtgc agaccacgaa cgaagtcgtt ggaggtgtca cccagattct
     7741 taaatgcatc tgcacccata cgagtcatga tgttcatgtt cagaacaaca tagatggagt
     7801 cagtcagctc aacgccaacc tttgccagcg gagaaccgat cggacccata gaatacggga
     7861 tcacgtacat ggttctgccc ttcattacgc cgtcatacag cggaatgagc tttgccttca
     7921 tttcttccgg atccatccag ttgttggtcg gacctgcacc ttcctttgtc ttagagcaga
     7981 taaaggtacg atcctctaca cgagcaacgt cgttcgggag tgtgcgatga tacagacagc
     8041 ccggcagctt gtccggattc agcttgtaca tcttgtcatg attgccttcc ggcagagaag
     8101 ttacttcttc tgtcagtgca tccagctgtt ctttagaacc gtcgatccat acgaccttag
     8161 ccggctttgt cagagcgacc atttcgtcga cccactgcag cacattcttg ttgttagtca
     8221 atgccattgg tactacctcc tgataatttg cagcaatctc attgcatgct ttttttgggg
     8281 aacttcttgc aaaattctcc aacactttta ttatacccaa attcgcctgt catgtcaagc
     8341 agtttctgca aaaagccact aattttcaaa atgcgttcat gaaacagaat catctgtttt
     8401 cgacacgtgg acaaaatatg gcaggtcaat accgcacaaa gattgtgcga cagatgcgtc
     8461 gtcaaatttt tcgtcagatg nnnnnnnnnn gtcgccagac gctctttgtg cggtattgcc
     8521 gcaggtatcc aacagaatta ccggcggcag ttcaggcact gtgcagacat tttcgagatt
     8581 ttcaaaatcc ccgtttacat tccgccaaaa ctatgttata atatagggca acaccgcaca
     8641 aagtatcaaa cattatcata aggagaaccc tcatgcagac aatacaaacc gcattcaccc
     8701 tcggaaagac cgccctgccc cgcacggcaa ttctggcacc aatggcaggc gttgccgacc
     8761 gtgcctaccg gctgctctgc cgcagcttcg gggcagctgc caccgtcagc gaaatggttt
     8821 ccgtcaaggg gctgtgctac ggggacaaag gctccgcaga actctgcacc attaccgagc
     8881 cggaacgccc catgggcttg cagctgttcg gcagcgagcc ggaattcgtg ggaaaagccg
     8941 ccgcactggt gcagcagtac gaaccggact ggatcgacat caacatgggc tgtccggtac
     9001 ataaggtagt tgccaccggt gcaggaagtg ccctgatgcg gaacattccg ctggcacagg
     9061 agatcgtgcg ggctgccgtg cgggagagca gcgtaccggt cacagtcaag ttccggctgg
     9121 gctgggacga gaacagcatc aacgccgtcc cctttgcaca ggcaatggaa gctgccggtg
     9181 ccgccgcaat taccgtacac ggcagaacca aatcccagct gtacagcggc aaagccgact
     9241 ggaacgccat tcgggaagtc aagcaggcag tgtccattcc cgtcatcggc aacggcgatg
     9301 tgcagactgc ggcggagtgc cttgcactgt atgagcagac cggctgtgat ctggtcatgg
     9361 caggacgggg cacctatggg aatccgtttc tgtttcggga gattcaggcg gcaatgtccg
     9421 gcgaactggt agtgccgcct acgctggaag aacgcatgga aaccatgctc cggcatgtgc
     9481 ggctgattct ggaatgcaat ccccagaagc cgccggagat cgccattcgc gatgcccgaa
     9541 agcatgcggc gtggtacatg accggactct atggggcagc acagtttcgg gcaagatgct
     9601 accacctgga ttcttacgcc gccgcacaac agctggcgga agattttttg cagctgcaaa
     9661 aacagcacca tgaaaaatag aaaaacacga aaggcagtgg gaaagaattc ccgctgcctt
     9721 ttttagaatc attccatgag aaagagaaac tacccctgca ctgcttcccg ctttttccag
     9781 ctgacaaacc cgtacaggtc attcagaaag aaaatcgcaa agttcactgc caccgggata
     9841 tacgccggat ttttcagcga tgccagcacc cacagcacga tcagcacaat atcgttcatg
     9901 gcatacccca acgcataata agaggaacgc agcatggtta gcgatgccgc caggaaactg
     9961 gtggtaacgg aaatggtact gaacacgata ttcggcgtat ccagcatgtg caaaatgtag
    10021 taaaacgccg cagttaccgc cacgctgaaa acagaaagcc ccgcaatatg tttccggttc
    10081 agcttctgga tctccacttc gtttccgttt tttgacgggt tccggatcca cgtgatcgta
    10141 gaccagaccg ccatgggcat ggtcatgccc agataagtaa tcatctcacc ccagtagcgg
    10201 aaccgccacg aaatgatgcc gtacagaatg ctgaacacga tcatcagaat ctgtgcccag
    10261 acgtttccct ttgcggcaaa gatcagcgaa gtaatgccca cacagacggc aaccagtgtc
    10321 agaacgtcca cactgccgga taccagattg gaaagaatga cgatcaacag cgatcccagc
    10381 cagaggagcc attccttttt ggtaaggtct tgtattggat tatgcatcag agatctcctt
    10441 tatggcaata ccgcacaaag attgtgcgac agatgcgtcg tcaaattttt cgt
//
LOCUS       DAWBGR010000079        10423 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1850_length_10423_cov_5.274306, whole genome shotgun sequence.
ACCESSION   DAWBGR010000079 DAWBGR010000000
VERSION     DAWBGR010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10423)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10423)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10423
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1850_length_10423_cov_5.274306"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2520 a         2791 c         2549 g         2563 t
ORIGIN      
        1 cgaaaaattt gacgacgcat ctgtcgcaca atctttgtgc ggtattgcct ttatcatagc
       61 acagtcgggt tccatttgtc aagaaatgcg gcaagggggg aatttggatt ttcaggaatc
      121 cgcgaccgac agtgccacaa gcaaagaaac caaaaaacgc cctctgcaca tcatgtccag
      181 agagcgtttc cttgggaaac actgcataaa gcccgcctat cagctttgca cacaatctga
      241 cgacgcatct tacgcacaag cttttgcagt gtttcccttg tatttttatt gcaataacgg
      301 attgcttacc gttccagaat ctgacttctg tccggtccgt tgccgaccat gactaccttg
      361 cactcgcaga cttcttccag ccgtgcgatg tacttcttgc aggcttccgg cagttcgtca
      421 aagctcttgc agccggaaat atcttcgtca aagccttcga attcctcgta gatcggcgag
      481 cagtctgcca gagattccag tgctgccgga aaatctttca gtactgtgcc gtccggtttc
      541 tgatagccaa cgcacatttt cagggtgccg atgccgctga gggtgtccag cttattgatt
      601 gccagaccgt ccagaccgtt ggtgcggaca gagtgccgta caatgacagc atcgaaccag
      661 ccggtacggc ggggtctgcc ggtagtggta ccgaattcgc cgcccttgtt gcggatcgtg
      721 tcgccggttt cgtcattcag ttctgtcggg aacggtccct tgcccacgcg ggtggtgtat
      781 gcctttgcca cgccgatgat gttggtgatc atcttcgggc caacgccggt accaatgcat
      841 gcacctgctg caatggggtg agaagaggta acatacggat aagtaccgaa gtcaatgtcc
      901 agcagggttg cctgtgcacc ctcgaacata atggtctttc ctgccttgtt tgcctcatag
      961 gtcagaacgg aaacgtcgtc cacatacttt gccagacgct tgccgtattc ggtatactct
     1021 gcaatcactg cgtccagatc aaatgccttg ccgccgtaaa cagcagtgat gatcttgttc
     1081 ttcagtgtgc cggtagtccg tgccttttct gcgaaaattt ccggatgtac cagatcatag
     1141 atccgcagac cgcagcgttc gtacttgtcc atgtaagtcg gaccgatgcc acgcttggtg
     1201 gtaccgatgt ccgagttgcc gcggaacttt tccgacagac cgtccagctc aatgtgccac
     1261 ggcattacca cgtgtgcacg gggatcgatc ttcaggtttg cggtagaaat gccgcggctt
     1321 tccagactgt ccagttcaga gatgaagtcc ttcgggtcca gaaccacgcc ggcaccgatc
     1381 aggcacaggg tttccggata gagaataccg gacgggatca ggtgcagctt gtacacttcg
     1441 ccgttgttga caacggtgtg accggcattg ttgccgcctg ttgcacgaac caccacatct
     1501 gcacgagatg ccatgatgtc gatgatctta ccctttcctt cgtctcccca ctgtgaaccg
     1561 actacaatat ttgcaggcat aagaaattcc tcacttttca aaataggatt tgtttccgga
     1621 atccagcttc cggtgcagtg tatcactgca gcatactatt ctattataac aggctttttc
     1681 gggaaatgca atagcatttg cagatttttt tctttttgtg gggtttctgc ggggaaatac
     1741 gggaaatgtc accgccgttc acccgcaaaa atccaccgct tgtcgcaaac cgcttgaggc
     1801 gttctgcaaa atatgctatg atatagacaa caccgcgaaa ggaggaacaa cgccgcgtga
     1861 agatacaaat acaactggac agccagtgcg aagaaccgga gattctcatt cgcaccgccg
     1921 ccgtaaccgc agagattcgc gaactggtgg aaaaactgtc cgaaaagcgt tccgattccg
     1981 ttgtgggcta tgatgcagac agtgccgtcc tgctggaaaa atcccagggc aataccgcac
     2041 aaagtaaaaa cagtgaaaat accgcacaaa aacgcctcct gcacccaacc gtgcagaagg
     2101 cgtttccttg tatcgtttta cttcgagaac cgtccgtcct cgtccttgat cttgctccac
     2161 atataccgca gcatccagcc gtcgaagtcg gtgatcttca tggcagagcc tgccatcatc
     2221 agagaatttt ctccgccggg gaaaccgccg ggcgggaagc cattggattc gccttcgccg
     2281 ccgtaaaagt ctgtcatgcc gaaaccgtga ccgatctcat gttccagcac gtgaatgcct
     2341 gtgccgtcca gcatgttgag gtacgctgca tccgaaagtc gctgtcccca gtcaccgccg
     2401 cagccgccga tgttcgggaa gccctgtgtg ccccacaggt acatatcaaa gcggctgtcc
     2461 agcccgttgg ggtagacata gctgctgtct gcaaagtggt caaaacggga gatttccgac
     2521 ggggcactgg gcagcttatc cggaatggta tccacgccgt tggaggtatc ataggtgctg
     2581 tcatagtcgg agatcaggtt gtcatagaca acttcgtccg gctgcaggtc ttgcagaacg
     2641 gatttgtcca gcactgccca gcctacaatt tttacgtcca cgtggtcata cttccagccg
     2701 tcccagctct tgagccagtc gttccagccg ttgatgctgt cgctgaccag cttttcaaac
     2761 tgctgccgct gttccagtgt cagcgtccgg taggactgcc accgtaccac atagttgatc
     2821 gtaccgtttc cggcatcgat ctggtcaaaa atggtgtttc gccgttccgt ggacttttcc
     2881 gcagtaatgc ggttgttcca gatccagtct gccgcatagg tgtaatctga cgggaaatcc
     2941 gccatagtca gctcctgacg gggtgcttct gtggtggcag tcactgtagt ttccgtcgtt
     3001 gtcgttgtag tcgtagattc ctctgtagtc gtggtgacaa ccggttctgt cgtcaccggt
     3061 tccgtcggct ctggtgccgg cggcacaatc gtacatgccg gcacggaaac cggtgcaagc
     3121 gaaaccgatt ctcccagcag atactgctgg agcagtttca catcagcagc atctaaaaca
     3181 ccgtccccgt tggcatcgcc cacagcctgc tgcataaagc tgacctgtgg ctgttccact
     3241 gcctgccgca gctgcaccag atcggtgccg tccacaacac catcatcgtt ccagtcgccc
     3301 atgtgcagat tggtcactgc ctgcggtgtc acctgtgcat agaccggcga ctgcacaccg
     3361 ctgcggaatc cccgcagata gccgtcctct ccgaattcaa actgccccgc atttgtggag
     3421 atctccagcc tgttttcctt tgtgttttct gtcagatagt attcgccttg ggacagtgcg
     3481 gatttgatcg taacgcccaa gttggtctcc gtgaaatacc acgtgttgta gggtgctgca
     3541 ccgtctgcct cataaaagcc gacctgtaca ccctcaccct cggtgccgcc cagcacactc
     3601 agatagcggc cggtgctgtc ctgtagcatg tatcccgtcc aggtgttgta cgattccgat
     3661 gatactgcga tcaccggaac ggacactgcc cccagaactg ccgcagtcag ggcagccgca
     3721 cagcgtcgca gcgtttgttt cttcttcata ctcaaattcc tcccgaaccg aaacgccttt
     3781 cggttctgct ctcggagcga tcgcagcact gcacacgctc ccatttctaa tttccaataa
     3841 gcctattata gcacatcgaa atcataaaag caacaattat ttcctgtcat gaggaatact
     3901 tttattgaaa gaacacatct gcaacaaatc tgacaacatg ccccttttca atcacccttg
     3961 gcaatttcac cggaaatctg catgaaatct cgtacaaatt gcggattgca cccatgcagt
     4021 atttcatgtt ataataatag tatatataaa gcggcactgt atcagacgaa atttgcgtca
     4081 tgctgttgtg agcttgtgta agacaggaga acgtttatga aaaaaagtgc aagaatcgcc
     4141 ctgcactgtc tgctggcaat ggtactcacc gtaccgctgg cggcatgcgg ttcggatacc
     4201 aaagatacca ccccgaaaaa agatatgtcc accaacacgc gggaacagct ggcagaattg
     4261 tccaagtcgg acgaacggct caccggtgag ctggaaaaca agacgatcaa gtggatgtca
     4321 gactgggaca tcaactccga cgataccgga aagaccacgc ccatcgaact ggcagtgttt
     4381 caggaacgct acggcggaca gatcgagttc caccagacca cctatggtgc acggtatgac
     4441 aacctcgcca acgccattaa cagcgatgag ggcatcgact ttttctacgg cggcaactgg
     4501 gacgcattcc ccaagggtgc tgtccgcggc atgtttgtcc cctatgatga ttacattgat
     4561 ctggactccg acctgtggaa agacgtaaag gctgccaatg atgccatggt ctggaacgac
     4621 aagcactaca tggcaatcgt agaagtcacc ggcgaccagt gtgccattat ctacaaccgc
     4681 aagactatgg acgaaaacgg tctggacgat cccgcagaac tgtttgaaaa gggcaagtgg
     4741 gactgggacg ctttcgagga tatgctctcg gaattcgtag acacctctga cgaacgctac
     4801 ggcatcgaca gctggtattt cgaatccggt ctgtccgcct ccaccggtgt tccctatatc
     4861 ggactggaaa acggcaagct ggtgaacaac ctgcggaacg aagccattga agattaccag
     4921 aactggatgt acgggctgtg gcagtcggac tatatcgctc tgggtgtcgg cgactacggc
     4981 tggacctcgc gggactacta catcggcgag ggcaagctac tgttctaccc ctgcgggctg
     5041 tgggcactgg gcaccgaaaa atcccagtgg tccaagacgt tcggcgagga cgtattcttt
     5101 gtaccggtgc cgaagtacga caaggcagac gaatactata tccccaccag tgtcaacgcc
     5161 tactgcttcg tcaagggcgg acacaatccg gagggcgtgg gcaagttcct cgactgcttc
     5221 cggttcagca agatcagcga cggtgcacag gagatcagcg atgaacagtt cgtcaaggac
     5281 tacgagtgga ccgacgaaat gatcgagatg aaaaacaaaa tgaacgaaat ggctctggcg
     5341 aatccggtat tcgactacta caacggcatt tcctccgatg tcaccgacat tctggacagc
     5401 agcgtcaacg gcattcgtgc cacttcccgc ggaattccgt ggaacgaatc tgtcaacgcg
     5461 atctatgacc agacacagaa cttcattgat gaggtcaacg aaaagaacgc tgctgaataa
     5521 tcccctagta aaccataaca actctgaaat attgcaaaca accgccgaca gaagatttcc
     5581 tctgccggcg gttgtttttc cacaaaaaag ccggaacaca ggtgaacccc atgttccggc
     5641 tttctgtatg cgattaaatt gtcagagaag ttctctcaca gtttttctta gtacataccg
     5701 cccatgccgc ctgctgccgg agctgctgct gccggttctt ccttcggaat gtttgcaacg
     5761 gcactctcgg tagtcagaac catagaagcg atcgatgcag cgttctgcag agcacttctg
     5821 gtgaccttgg tcgggtctac gataccattg ctcatcatat cgcaataggt ttcgttgtat
     5881 gcatcgaagc catagccaac cttgcggctg cggcgaatct tatccacgat gatgctgcct
     5941 tccagaccag catttgcagc gatctgacga agcggagctt ccagtgcacg gagtacgatc
     6001 tttgcaccgg tcttttcatc gccgtccaaa gacggaacca gcttttctac tgccggaatt
     6061 gcattgatca gtgcagtacc gccgccggca acgatgcctt cttctacagc tgccttggtt
     6121 gcagacagag cgtcttcgat ccgcagcttc ttttccttca tttcaacttc tgttgcagca
     6181 ccgaccttga tgactgctac accgccggac agcttaccga tacgttcctg catcttttcc
     6241 ttgtcgaaat cagaagtgga agcagcgatc tggttcttga tctctgcgat tctctcctcg
     6301 attgcttccg aagtgcctgc accgccgacc agaatggtgt tttccttgtc gatcactgcc
     6361 tgactaacct tacccagcat ctcaatgttt gcatcagcca gttccatgcc cagatcagaa
     6421 gacagaacag taccgccggt caggatcgca atatcctgca gcatttcctt tcttctgtcg
     6481 ccgaagcccg gagccttgac agctgcacac ttaaaggtac cgcggaggtt gttcaggata
     6541 atagtggtca gagcctcacc ctcgatgtcc tctgcaatga tcagcagctt cttgcccatc
     6601 ttgacgatct gttccagcat cggcagaatg tcctgaatgt tgctgatggt cttatcggtg
     6661 atgaatacca gagcatcatc ataaacgacc ttcatcttct cacggtcagt aatcatgtac
     6721 ggggacatgt aaccgcggtc gaactgcatg ccttctacga cttcgctgta ggtttccgca
     6781 gtcttgcctt cttcaatggt gataacgccg tcggtagaaa ccttttccat tgcctctgcg
     6841 atcagcttgc cgacattttc atctgcggaa gaaatggtag caactcttgc aatatcctct
     6901 gtgccgttaa cgaccttgga attttcctga atgaccttta ccgcagtgtt cactgccttt
     6961 gcgataccct tgcggagcac catggggttt gcaccggcag cgatgttctt tgcaccttcg
     7021 tgaatgattg cctgtgccag aacggttgcg gttgtggtgc cgtcaccggc agcatcattt
     7081 gtcttggtag caacttcgcg aaccagctgt gcacccatgt tttcaaacgg gtcttccagt
     7141 tcgatgtcct ttgcgatcgt cacgccgtca ttggttacca gcggagcacc gaattcgcgg
     7201 tccagaacca cgtttctgcc cttcggtccc agtgtgatct ttacggtgtc tgccagcttg
     7261 tcgataccgg cgatcagtcc gtttcttgcg tctgcattgt acttaatatc ctttgccata
     7321 ggtcaaaact ccttttctct gttgtggaat cgttcttgtg ggatcactta ttctgtgatg
     7381 cccagaatct cgctctcata taccagtgtc agttcctcgc cgtccagctt gacggtctga
     7441 cctgcactgt ggctgatcag aacggtatcg ccaaccttga cttccatggg aacgcgtgtg
     7501 ccgttcgggg agaacttgcc ttcgccgcat gcaacgacct tgccctttgc gggaacgcct
     7561 gccggattct tggtcgccag taccagaccg cctacggtct tttcttcttc tttttctact
     7621 ttaatcagta ctctgtctgc caacggtttc attgtcatga tccattcctc ctgcaacata
     7681 acattatcac ttgttttcga aatgtgttag cactcaatat tcccgagtgc taatatctat
     7741 tttaccaact tttcagaaaa aatcaagggc ttttgtgcga ttttccggaa attcagacat
     7801 tttcacaaaa aataaatctt tccaacgcta aaacaacagc aatatccaca aatgccctgc
     7861 catgaaatct tgaaaaaaca gctgtaaatc tgtttctatg caaaaataaa ctaacaaata
     7921 cacgtgtaga aatcactatc ttcacatttt acaatttaaa cataattgag aaataaaata
     7981 tacacctatc aatcacagtg ctatagtata ataatattgc aagaatgtga cagcgaccga
     8041 cggaatttcc gcggtcctgc cggaagcagc tctgcttctc ttttgtatag cccccaatat
     8101 tcgtacaaaa aaagaagcaa aaaaacaaat ctattctgaa ttatgaggaa ggcgtgaaaa
     8161 atcatggcat tggaaaaagt tcttgttgtt gatgatgacc cgaatatttg tgacgtactg
     8221 cggatgtatc tggaaaatga aggatacagt gtaattctgg catacgatga tgaagaggct
     8281 ctggtaaagt ttaatgcact gaagccggac atcattctgc tggacgtaat gctgcccact
     8341 ctggacggct ggcaggtttg ccgcgaagtc cgcaagaagt ccagcgtgcc gatcatcatg
     8401 attaccgcaa agggcgacac ctttgataag gtactgggtc tggaactggg tgcagacgac
     8461 tatatcgtaa agccgttcga caccaaggaa gttatcgcac gtatcaaggc aattgcaaga
     8521 cgtgtgggca attctccgga ggaatccgaa gtcaaggaag ttcgctatga caagctgtcc
     8581 gtaaacatga cccgttatga actgcgtgta gacggcaagg tcgtagacac cccgccgaaa
     8641 gaactggaac tgctgttcta tctggcatcg aacccgaacc gtgtatacac ccgtgaccag
     8701 ctgctggacg aagtatgggg ctttgagtac tatggcgact cccgtaccat tgatgtccac
     8761 atcaagcgtc tgcgtgaaaa gctggaaggc gtttccgaca agtggtcttt gaagaccgtc
     8821 tggggtgtcg gctataagtt tgagtctgaa gaagaagact aagtcccgtg gaaaaagcag
     8881 aacaaagtcg aaaaaaaagc caccactggc tccgcatctg gagagccttc ttgatccggc
     8941 tgctgctggt cgctgtagtt ctcattatta cggtaactgc actgtccggt tatgcgatct
     9001 ccaagtacaa gcaggattat tacagcaatt ccatgcgtgt ctgcgatatt atggcgaagg
     9061 atttcaccga atacaacgcc cagaacccac agctggatct ctggcaggag tatattccga
     9121 aaagtgtgaa taaagttgcc gaacagatgc acgtttttct gtacgtgttc aatgcagacg
     9181 gcagttgccg gatcgcaagc agcaacagca gcatcgcggc agcagacctg accctgaaca
     9241 acgaaatgct ggcgggcata cggaaaggcg gcgaatacct gacggacaaa gcctccggca
     9301 agatcgatca ttcgatcgta gagccgatgc tcacccgcgg attcacattc accctgacgg
     9361 aaaacggcgt agaagccact tattatctgt ttggtgcatc atacaccaag cctttgaatc
     9421 tgtataccac acatctcttt ttgtttatgg cgatcctgtc gtttacactg ctgctgattg
     9481 cgggcgttct gatcttttat caggtatggc gaggcaattc gcagctagaa cagctggaag
     9541 ctgcattgca gcgttatgca cagggcaact acagccagcc cattcctgta gagcagtata
     9601 ccagttccaa tctggaactg attgccgcac tcagtgcaca gatcggcagt cagtccggac
     9661 gggcagaaga atccagccgc cagtttgtgt ccaacgtttc tcacgaactg cgtaccccga
     9721 tgaccattat cagcgggttc gtagagggca ttctggacgg cactgttccc aagaacaaac
     9781 gcatggaata tctctctatt gtgtcgcagg aagtccagcg gctgaaaatg ctggtcactt
     9841 ccatgctgaa tctaacaaag tttgatgccg gcaccattca gctgaattac cgtatgttta
     9901 tcctgaacga cactgcattc cggacgattc tgatgtttga aaaccgactg gaaaagcggc
     9961 atatttccgt agagggactg gacggcgagc cgattcgcgt ctgtgctgat ccggatctga
    10021 tcggacaggt ggtctacaat ctggtggaga acgccgtgaa gttcgtcaac gacggcggca
    10081 cgatctcgtt cacctttgca gacgaggaag actgctgggt atttgcagta cgcaataccg
    10141 gaaacggtat ttccaaagaa gaactgccga aaatctttga acgattctac aagtccgatg
    10201 cctcccgcag tcaggacaaa accggactgg ggctggggct ggatattacg aaaaaaatca
    10261 ttcacctgca ccacgcacag atcagtgtcc gcagtgagga acatgcctat acggagttcg
    10321 aagtgcgttt gccgcacgta gagccgccgg aacaggaaca cgaatgatat aaggcaatac
    10381 cgcacaaaga gcgtctggcg actttgcacg ccatctgctt gca
//
LOCUS       DAWBGR010000080        10389 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1860_length_10389_cov_5.611186, whole genome shotgun sequence.
ACCESSION   DAWBGR010000080 DAWBGR010000000
VERSION     DAWBGR010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10389)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10389
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1860_length_10389_cov_5.611186"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2598 a         2892 c         2542 g         2357 t
ORIGIN      
        1 gcaagcagat ggcgtgcaaa gtcgccagac gctctttgtg cggtattgcc ttttgtatct
       61 gagagccgca cagtgcctac agacaaaaaa acgcccgact ccaaccggaa tcgggctaaa
      121 caaaatcaag aacaaaagaa aggagacaac aaaacgagtg ataaaatcaa tggagaactg
      181 ataaaaacaa aataggaggg ttttcaccaa tcataagacc tccatctttg cggttttatc
      241 accgtatcta ttatacacaa ttcgccgtca cccgtcaagg gaaaatacac aaaaaatgcc
      301 tgttctctgt ggattttgta gaattgcaca aaaagtatgg tacgccattt gtggttgtta
      361 tcaaaggcaa taccgcacaa aatacatacc gtcagaaaac gggtgttttc cacagaatat
      421 tccacacggt ttgcgggcat actataagag caaaactgtg agaagcggat atgtattctc
      481 acagaaattg cagaaaagag gtgtagctgc atgtgggata aacttgcact gatcctgctg
      541 attatcggcg gtgtcaactg gggactggtc ggcgtgtttg aatttgatct ggtggcatgg
      601 atcttcggcg gttcggcatc gttcatcagc cgtgccgttt atattctggt ggcgatcgca
      661 gccctttggt gtatgacatt cctgttccga cgggaagcac tgaccgagcc cgcgacggtt
      721 tcccagcgga gatagggaaa cactgcatca agcctgcacg cagatttcgt gcaaagccgt
      781 caggcgggct ttatgcaggg tttccatgtg ctttccacat tccactgtgg aaaacagaaa
      841 ggtctgtccg gacaagggca gacttttttg tgcggtgttg tccatgcaca tatttcccca
      901 ggcaatcgca tagattgtgg aaacgcggaa cgtgttcaca tcaacgcttt gacaggaggc
      961 taactgctat ggaaaaaaca ccggaagaat atcaggcaga gctgctccgg ctctatcaca
     1021 cggcacaggc atctgcacca gcaccttcac ccgttccgga accggaagaa gctccgccgc
     1081 aggagccaca gccggaaccc actcccccag aaccggagcc tgctcccccg atgccggaac
     1141 cgcttcctac cgaaaccgtt cctccgccgc agctggaagt ggagaaaact ccacaggaac
     1201 agccggcgat cggctggatt caggtcatca cccgctgtgc cggcaatgcc cgtgcccttg
     1261 ccggtgtgtc cgtgctggtg accaacgaca cggaggaaca agtacatctg gaacacactg
     1321 ccgtcaccaa cgaaagcggc gaaaccggaa aaattccgct gccggtgccg gcggcttctc
     1381 tctcactgaa ctgcgacgaa acccgaaagc catatagcac ctatgatgtc agcgtctatg
     1441 cggacggttt ttaccggcag gtcagcgaag ctgtaccagt attcgccggc acgacctccc
     1501 gacagatctt ccacatgatt cccctgccca gctacttgca ggagccgccg aaaaccattg
     1561 tctaccaaaa caccgaaccc aatctgtaag gagggctgcc agatatgcca gtcggagaac
     1621 cctttatccc cagagatatt accgtgcatc tggggcggcc ggaggaaacg gcgaacaacg
     1681 tcaccgtttc ctttcccgat tacatcaaaa acgtggtatc cagcgaaatt taccccacgt
     1741 ggccggaaaa tgccattcgt gccaacatct acgtcattgt cagctttgcc ctcaaccgtg
     1801 tctataccga gtggtatcgc tcccgcgggt atccctttga catcaccaac tccacccagt
     1861 ttgaccagaa gtacatctac ggcagagaaa ttttcgaaaa cgtggggcag ctggttgacg
     1921 aactgttcaa ctcttacgtc cggcggcagg gcaatgtgga gccgctgttc acagcgttct
     1981 gcaacggcac aacggtaacc tgtgacgggc tttcccagtg gggcaccgtg ccgctggcac
     2041 aacagggcat gactccttat gagatcctta ccacatttta cggcagcgac atcgacattg
     2101 tcaccaacgt tcccgtaatg accaacacac cctcctatcc gggatttgat ttgcggctgg
     2161 gactttccga tgatccggtc cgcaccattc aggtacagct gaaccgcatt tccagaaact
     2221 accctgccat tcccaaaatc ggcgaagctg ccggcgattt tgattatatc acacaagatg
     2281 ccgtcattgc ctttcaggaa atcttcgggc tgccggcaac cggcattgtc aacgaagcca
     2341 catggtaccg gatctcctat atttttacca gcgtgaaaaa cctggcagaa ctgaactccg
     2401 aggggctgac ccagcaggac atcgagctta cgcacaccga ggatctgcac ttcggcatgc
     2461 agggaaaaaa catccgtgcc ctccagtatc atcttgcagt ggtgggagcc tactatgccg
     2521 ccatacagcc ggtggaaatt accggctact tcggagaaca gacagaaact tccgtcaaat
     2581 ccctccagca ggcgttcggg cttccggtga cgggcgtggt ggatttttcc acatggaacg
     2641 ccctctatga tgcctacacc ggcattgtgg aatcgatacc gctggacacc tcggtcaatg
     2701 acgtggtgct ctaccccggc gtggttctgc gggagggcac taccagcgag tacgtccgtc
     2761 tgatgcagca gtatctcacc tatatccacg gcacctatcc ggaaattccc accgtcaacg
     2821 ccaccggcta tttcggcccc atgaccaagg cttctgtcat ggcgtttcag gaactgttcg
     2881 gctacccgaa aaccggcgta gtgggtaccg cggtatggaa cgcgatcacc agcctgtact
     2941 ccgatttgaa atacggctac gacaaacaac cctatcaggc tccgggatat acgatcacct
     3001 gaggaaacac tgcataaagc ccgcctgacg gctttgcacg caatctgctt gcagattttc
     3061 cgtcatattg cccagaaatc ccttgccata catgaagtat gcctgcggaa tttctatgca
     3121 atctgccgaa aaatctgact acgcatctta cgcacaaact ttattcagtg tttccttaag
     3181 gagggtgaca catggcgtac acagagttac agaagcaaca acacattcgg gaattacagg
     3241 ggtatctcca cggcatttcc cacatacagc agctgccaca tataataccg gacggcatct
     3301 atggagagaa aaccattgag gcaatacggc agttccagca gcagaaccac atgccggcaa
     3361 ccggtgaaac tgacactgcc acatggaacg ctgttgcaca ggcatatttg caggaggttc
     3421 gtccaccggt actgcatctg gcactgtttc ccaccggcat caccgcctac acgctgggcg
     3481 atacgggcgg tgctgttttc gtgatacaag gcattttgca ggcaattcag aacaattttc
     3541 cgaagatccc gccgatcagc aacagcggtg tatttgacac tgccacccag aatgccgtga
     3601 agcagttcca aagctacacg ccgctgccgg cagacggtgt ggtagatgct gccacatgga
     3661 atcatctgct ggcggcactg gacgcggaga tccggaattc tgccggcgta gtataagaca
     3721 gaaaaaagga gttaccttgc ttgagagcaa agtaactcct tttgttttac acgttcagaa
     3781 aacgatattc tttggaatag ggcttcacat gtgcagggtg ctctgcggca tatgccagca
     3841 gcagcttgcc caagccttct tccgccagaa atgtttccag ctgctcatcg gtatagcgtt
     3901 ccatgccggg acaatatccc gccacagcga cacgcacatt gccagccaaa tcccgcatca
     3961 tgcggaacgg ccagaccttg cagtcaaagg gcttttccga ccccatggta cagccggttt
     4021 cgcagagcat gggacagaag aacagttcct cgccccgcag cggcggtgcc gcaaatgtct
     4081 gctcctctcc cgccggaacc agcttcggcg aaacacccat acggtgcatg gcttccaccg
     4141 tttcctgcgg cagtaccgga aattcccacg tgtctgtaca gtcaaacccg cagcagagcc
     4201 ggcaggctgc acaggtttcg ggagaaagaa tctttcgcag catgctcagt cgatcggtac
     4261 gatccagccg aattcgtccg gaatcttgcc ccactgaatt ccagtcaggg tatcatagat
     4321 catctgggaa accggtccga tcttgttgtt gttgatcgtc atgaccttgt cgccccactt
     4381 caggtgacct accggagaga tgaccgcagc ggtaccggta ccgaatactt cggacagctt
     4441 gcctgcgtca tatgcatcgg caatttcctg aatggtgatg cggcgttccg aaaccggaat
     4501 gcccttggaa cggcacagtt ccagagcaga ctttctggtg ataccggaca gaatggagcc
     4561 ggtttccaaa gacggtgtta ccacttcgcc gtcaatgacg aaagcgatgt tcatggcacc
     4621 aacttcttcg atgtacttct gctctacgcc gtccagccac agaacctgcg aatagccctg
     4681 ctcataagca tcagcctggc tgtgcagaga agccgcatag ttaccgccgg tcttggcata
     4741 acccataccg ccgcggacag tacgaacgta cttgttttct acgtagatat tgaccggatc
     4801 cagaccggaa gcatagtacg gaccggacgg agaaagaata atcatgaaca gatagtgttc
     4861 acccggatgt acgccgagga acggatctac tgcaaaaata aacggacgaa tgtacaggga
     4921 agcaccttct gtgtgcggaa cccaatcctt ttccacagag atgagttctt tcagacactg
     4981 cagtcccagt tcctccggca gttccggaat gaccagacgc tcgttggaac ggttcagacg
     5041 cttgaagttt tcgtccggac ggaacagctg tacaccgccg tcggcacggc gatatgcctt
     5101 cagaccttcg aacacggtct gaccatagtg caggcacatt gcagacgggc taagggtaat
     5161 atcaccatag ggcatgatca ccggatcgtg ccagccctgt ccttcatcat agggcattac
     5221 cagcatgtgg tcagtgaaga tatgaccgaa tccaagcttg ctctcgtcct tgggtttttc
     5281 tttcggacaa gtcgtcagtt gttttttgat ttccatgtca aaaacctctt ttttgatagt
     5341 tttgccgcaa atcgctgtgc ggttggacgg ctctgctact attatagcac acttttctgt
     5401 attttggaat agttttttga aatttttttt ggattttcgg attctttttt atggtgcggg
     5461 gcaaaagtca ttctgcaagt ggcgtttttt ccctgctctt gtccttgaaa tacggttcca
     5521 gttccacttc cgagtcaata taaacggaac gaatgtcatt gctgttataa acccgaatca
     5581 tttttccgtc cagccaatac cgctgcatca gcgaaccgtc cacatcttcc agcagttcgt
     5641 ccttgaccag atgcgggaga tgcgtttcca gtgccctgca ctgtttctga aagatcactt
     5701 cgtctgcaat cgtgcagata ctgtaatggt acattgcatc tgacctccat actataggca
     5761 ataccgcaca aaaaatctcc gcacctcagg ggcacggaga tctcttacat atctgttatt
     5821 aaccttccca aggtcctgca tattccgact gcacgcccag atattcttcc agcgtcaaac
     5881 cctgcgacat gatctccttt gcggtatcca cgcccacata gcggatatgc cacggttcgt
     5941 actgatagcc ggtgatgttt tccttaccgt ccggaaaacg gataatgaag ccgtagtctg
     6001 cacaatgctc tgccagccat accgcttctg cggtttcacc aaaggcatca tcaatggtgt
     6061 tgcagtcaat ggtcagtccg gtctggtgct cggaatatcc cggacgggcg gaaaaggtat
     6121 ctgccatttc ccagccgtac agatcgttat agttgttgta gatcttcacc tgatatgcat
     6181 aatcgcggaa gtccgaaccg atatacaggt tcagatcctc cttggcagcg tcggctttca
     6241 tctgttcaaa ggcagccttt gtggtggaat ccagtcccgg atcatagctt tccggcaaac
     6301 cgtagctctt gtttacgatt aggatcccgt ctacgtaagt gcagccgtct ttctgttcca
     6361 gatgccggac tgtcaccgga acggtctggg caatggcatc atttcctttt acagaaacgg
     6421 taatgtcaca cttgcccgcc ttgacaccgg tcacagtgcc tgcattgtct acggttgcaa
     6481 ccgtttcatc gctgctgctc caaaggagat caccggcact tgccgcaccg gtggtggaca
     6541 tgttcaccgc aatgccggcg gtaccaccct ccagcactgc cagataagtc ttgtccacag
     6601 acagatccag aatgttctca ttggtggtgg cagtttccga tgcggcagtg ctctgtgcag
     6661 aggtgccctc cgcagaagcc acaacaccat tgccctccgg aatgatgctg gtgccccgtt
     6721 catcggagaa gaacagcagc cggactgcaa atcccagaat gataaacatc acgtataata
     6781 acactaaaat gacaccgatt ctggaaaact tgtcccagac gttgtctttg gttcgccttg
     6841 ccataacgac ttccctttct ctttctcatt tcttttctcc ggttttcctg ctttttctcc
     6901 aaattgtaat aattgcaaca aaagccggtt caaatcttac aaagtggtta catctcttgt
     6961 attataccac actttctcgc tcctgtcatt tgacaaattc cacaaatatc gcatctctaa
     7021 attgcgtatt ttcagtaatt tgtgcatttt gtcagaaaat tgctcttgta aactgccaac
     7081 ctgcacaaat tttacggtac atgtgatttc attcttgaaa acctgcacaa agcatgctat
     7141 aataaatagg aacgtggaca gaaggcgtgc cagactgtct gcaacaaagg aaccgtgcga
     7201 gccgaactgc acgaacggtt cgggaatttt tgaaagggga aactccatga ccaaacagaa
     7261 aaaaatcatt tccgtgctca ccgcagccgc attgctgtgc acgggaatcg gcactgccgg
     7321 catcagccag ccgctgacgg catctgctgc ggactcgatc gaatcctcta tggattggga
     7381 tacactgaac attgccggcg gcggctttgt ttccggtatc atcaccggcg acgatcagat
     7441 gtatgcccgt accgacgtag gcggtgcata ccgctatgac tacgaacaga aaaaatgggt
     7501 tcagctgctg gacttcttga acgaagctga ccgcggcttc ctgagcgttg atgccatgtg
     7561 cgtcgacccc aacgatgacg acaccctgta tctgctgtgc ggctgtgcct atttcagcga
     7621 tgcacggaca gctattttcc gttcccgcga tgccggtgaa acctttgaag agatcgacgt
     7681 taccgatctg attcaggtac acggcaacgg ctacggcaga cagtgcggtg aatccatcgc
     7741 agtggatccg gacaatccgg acatcatcta ctgcggcggc gatgccactg ccagcacctc
     7801 cggtctgatt atgagtgagg acggcggtga cacatggaaa gcggttgacg gctacggcaa
     7861 gctggacttg ttcgaatatg agatcaactg gccgacatgg acagagcaca aggtcaagac
     7921 gactgcggag aagtatgacg gcggagcaaa cggcgtttcc acgatcacga tcacagacgg
     7981 caaggtcttt gtgggtactt ctattaaggg taaggcaaat ctccacgtgg cagaagtcgg
     8041 cagcgacgat ttcaagcccc tgagcgagga actgcctacc gagcagatgc cttcccgtat
     8101 caatctggat ccggacggca acctgctgat cacctacatc aacggtctga tgttcgacag
     8161 cggcactggt tatgcttata agtataaccc caagaccgac gaactgaaag atattacacc
     8221 gactgcaact tccaacggta ctgccaaaaa gctgaacgtc ggctacggcg gtgtctttag
     8281 cgaccggcac gactccaaca agctggtggc aactacctgt gcacagtggt actcccagtc
     8341 ttggactgct gatgcctggg agagagatgc cattgcatgg ggcgaccgct tcttcaagtc
     8401 ggaagacggc ggcgaaacat ggacagaaat gactcccggc aactccacct cctggggcgg
     8461 tccgctggaa gcgaactatc tggaggacgg cggtcacagc tggattcggg acaaggcgat
     8521 ccactggtcc ggttctattg ccatcgaccc ccgcaacagc gaccagttct gggtggtttc
     8581 cggtaacggt gttttcacct gtgaggatac ctgggcagaa tgccccacca ttcgctttgc
     8641 cgcagacggc attgaagaag tggtatctct ggacttcatc agccgtccgg gcaaggatcc
     8701 ggtttcagtt atcggtgact atgacggctt ctatcacaat gcagacggca cagcgaccca
     8761 gctgtccccc agcatgaaca agctgactac caccaccgct tctaccggcg gcatcgcata
     8821 ctgcccggca aacccggatg tcatggttcg tctggcagag ggctctgcaa tgggctatta
     8881 caccacagac ggcactacat ggcaggaact gccgaacgta ccgctttccg gtgcaaaggc
     8941 tgccatcaac cagctggagg acggcaccta ccgtattctg gtcagcagca gcggcaagat
     9001 ctcctatacc gatgactacg gcaagacctg gagcaccgct tccaccagcg acagcctgtc
     9061 cagcaccatc tggatgtgcg tggacgaaaa gaacccgcag tacgtctatg catacggcta
     9121 ctactacaac cagtactatt tctacagcaa gcccaaggct gacatcaccg atgcccgcta
     9181 tattctgatg gtcagcgatg actacggcaa gacctttaag aacaaccaga ccgtttgcca
     9241 gtacgaccag tgcgacggag cataccgcat tgcttatctg gacgagggca cattcgccat
     9301 tgcagccggc tactacggcg catatctggt aaccgactac ggcaagacca ttaccaagat
     9361 ggacaacgtc agctactgca agaccatggg ctatggtgca gcagcaaagg acggcgatcc
     9421 ctacaccctg tatatgtacg gcaagccggc agattctgat ccggaaggca tctatcgttc
     9481 taccgactgc ggcaagagct gggtactcat caaccagaac cacctgtacg gcggtaccgg
     9541 caacggtaac tatctggtcg gcgacatgaa cacctttggc accgtttata tgtccactgt
     9601 cggctgcggc atcgtagtcg gcaaagtcaa cggttctgaa gacccgaagc cggtcaccac
     9661 agataccaca cagacaacca aagcaaccac cacaacaacc agcaccacaa ccgtttccac
     9721 agcggcttct acgaaggatt ctgctgcatc tactacaatt gctaccacga tcgtaaccac
     9781 actcccccag accactgact ccaccggcgt ttctacaacg gcttctggca agaacaccgc
     9841 cacagtgccg gcaggagcaa cactctatgg cgacaccaat ctggacggca gagtggacat
     9901 caccgatgca gtacttctga acaaggcagc tgccggagca gtatctctgg ccgcacaggc
     9961 tgcaaagaat gcagactgca atgcaaacgg tgaagtcggt tctgacgatg cggtttccct
    10021 gctgaagttc ttggtacaga tcatcaagaa cctgccggaa gttgcagagt aatccgaaca
    10081 aatttgcaat cgcgatcata aaaagcactg cccctgtaag cgtaatggct tacaggggca
    10141 gtttgttttg gggcatcatg tgttgaaaaa aacgtttgac aaacacccaa cgtttattcc
    10201 gaaataaccg ttctggtttg cagcttcgga cagttatttc cgtttgcatt aactgtccaa
    10261 ttggtttctc aacgaaattc ataccataat caaaagagca gataccgcaa agtacctgct
    10321 cttttttatc ttagggaaac actgcataaa gcccgcctga cggctttgca cgcaatctgc
    10381 ttgcagatt
//
LOCUS       DAWBGR010000081        10384 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1861_length_10384_cov_3.156840, whole genome shotgun sequence.
ACCESSION   DAWBGR010000081 DAWBGR010000000
VERSION     DAWBGR010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1861_length_10384_cov_3.156840"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2460 a         2446 c         2529 g         2949 t
ORIGIN      
        1 gccgtgaagc acctgatcac cgccgacaat ttgcggaaac tgtccagcca gtccagccag
       61 ttccagccgc tgttctgcca gttctttgga ggtggcggtg gcaatggcat aaggcagtcc
      121 ggattcaggg cagaattgca ggaattctgt aatgccggat ttctgttcta cgccgtgctc
      181 tgcaatatat tgggaaacca gttcccgccg gcggtcacgt actttgaaat agtcaaagtt
      241 ttctccgtaa agctgccgga aataaggttg ggcgtgggcg gcacagcagc ttcggacatg
      301 cagggcggct tcttcagaca tgggataccc cagttccttg gcactttgca gccagcagcg
      361 gaaatagatt ttttccgtat ccagcaatac gccgtccata tcaaaaatca atccgcgaat
      421 ggtgctcact ggttctgtcc tcctgcattg gtcagcatct gccgcagggc tgccaagtcg
      481 aaaccattta ccatggaatc cgtgtacacg tcagcacact gatatgcctg ctgggaaatg
      541 gtttcgctgc ccagcagata acggcacagg cgaacggcat cggcgatgtt gacggtaccg
      601 tccagtgtga catcaccctt ctgccatgtc ggctgtgcag aagtttctgt ggttgatgtg
      661 gttgtgtcac tttcgctgac agaagacgaa gctgttgttg tttctgtagt agtttcttct
      721 gacgattctg ttgcagaggt ttctgtagaa gtggtagttg cttctgttgt ggtcgaaacg
      781 gttgtttctg ttgttgcttc tgtagaagtc gtagttgttt caattgcagt tgttgtggtg
      841 gcttcggtag cggtagtcac ttctgtcgtt gttgcaggtg tcggtgtggg tgttgtaccg
      901 ttcaggtaag cagatacctg ctccgaccag tattttccca tgcaggcgta gccggcttca
      961 ttggggtgta cgccgtcctc taatcccgtt ttcatatcta caacgctgtt gatgtctgca
     1021 aaccgaattc gtgctccgac agcctgcttt ttctctacca gttctttgac agaggcattg
     1081 tagttgtcta cgctctgctc taccagtgct gtgaattctt ccggattgtt ttcataggaa
     1141 tagccgctgg actgatatgc ccagagccag tcgtgcttga ctgatacgtc aatatccggt
     1201 actgatgcca cgaacagcat gctgtcgctg tctatgctgt ccagcagctt gtctaccagt
     1261 ttttccagac ggtctgtaat gccggcattc tggttgtcca gcagatcatt ggtgccgatt
     1321 tgcagcagga caatatccgg atcgtatgct tcgatcatat tgcctgtgac attgttattg
     1381 taataggttg tgtcaaaaat agtttcatac agtccctgcc gacttccgat tttctcaatg
     1441 gcatagccgc tgtagccggc atgggcggga tcatattgga atgttacgcc gtcagaggtg
     1501 gtgtaggaaa cggaatcaga ccagctgttt ttcggtccca ccatatcaaa attggtaaat
     1561 cctttttgct gcatttcgta gcaaaagtat ttccgatagc cgttgtcccc gttgatatag
     1621 ccgtctgtaa tggagtctcc cattgccaga atccgtaccg gttctgtagt gtcttctgcg
     1681 gcaaccggtg cagctgccat accggctgca aggggcagta cgccgataca aaggcttgtt
     1741 aaaacggcaa gtattttttg tttcatcatt ttattcttcc tttcatgtaa ttccttattc
     1801 ctgaatcaat ttgacaagag catctgtgat tacagtgccg ccctcgtcat tcgggtgttg
     1861 tccgtaaacg gtttttgaag catctgacgg gtttgccaac agggaggcaa catcaaaata
     1921 agtcgctccg ttgtccttgg caatgccctc caccgtttcg ttataggcag ttcgaattcc
     1981 atccaattct ggtgtcatgt caaacggcgg agagttccac aaaatcgtgc gaatgccggc
     2041 atcggtacaa gttttagtga tcttccggac atctgcttcg atttcttccg gtgtggcatg
     2101 accggtgccg ccataaggtc cgctgatgat gtcgttggtg ccgaatgcca cggtaatcag
     2161 atctgcaccg gcaacagcac gattcagcca gttgccctgc cgagcacagt cagaggcacg
     2221 ggcatagccg attcccagat tccacaggct gtaatcttct gtacccaact gatccagcag
     2281 ctgggctgcc cagaactgat acccgaattc ggaagtctga cagccctgtg tgacagagtc
     2341 ccccagcgtg acaattcggg tcttgacttt tcggtcacag cccacaagct ggggcacagg
     2401 aatttcattg gtgtagagga atccctttcc gtccccttta tctgcatagg cataggtcag
     2461 gttagacatg gcaattgccg gaatatttgt tccattgact gtccattccc acagcagata
     2521 gtgcccttcc ggtacgttca gcgttacagg atcgctccag aaagtttcgt ccggagcgac
     2581 ttctttggcg gcactgccgg aaaaggtcac ggctgcggaa actattggtt ccacgttggc
     2641 atcgaattcc gtgccgccgt catagacagt tgccttttca atggtgtagc tgccgccgga
     2701 catgcccaca tgactttcgc tgccgtcacc ccatgtggaa tctaccgtat tggaaaagta
     2761 aaaccggtag tcatattccc ctgccgcttc taccggaaac catgcccgat aggtcacatg
     2821 ttctgcgttt ttgataacgg tattgctgcc ggtagaaatc acggtattgg acacgtaagt
     2881 atccagcaga gaaagttcct gatctgctgt tgaagtagtt tcggcacttt cttctgttgg
     2941 cgtatttgat gtgccgcagc ctgcaaaagc tatggtcaat gctgccgttg tcaggagtgc
     3001 caaaatttgt ttccgttgga ttcttttcat acaacgcctc ctgtcttttt catcttgact
     3061 cattgtagca cggcaatttt cttttgtcaa ggacaatttt atgctttttc tttttgcaaa
     3121 tttgtttttc aaagtaaatt tatgcacaat aaaattgcag tgctgttaga attttttata
     3181 tgaaacgctt gattgtagag caagaatgtg ctataataaa aatgttcgtg agaaaactgg
     3241 catgaaagat gtatgccaca actgaaaaaa ggagaattga aatgaaaaaa cgcatctttg
     3301 cgggaattac ggcaagcatt cttgcccttt cctgtgccgc acaagtgccg gcaattttgt
     3361 ccgcttcggc tgctggaaat tatgaaatgc agttgaacat caaattgaac ggtgaaaaga
     3421 aagcaatcag cccgtatatc tacggtgtga acgaagccgg aaattcaacc agtttgaaaa
     3481 aagtgacaac gcattcgctg cggcagggcg gcaatcgttt tactggctac aactgggaaa
     3541 ataattattc caatgccggt cgggactgga acgatagttc tgacacaaac atgggagatg
     3601 taacagacgg tccggcatat caggcacgcc gactgtcgaa ggaagctgct gaaaacaacg
     3661 tagactacaa aatggcaacg ctgcaaatgg cgggttatgc ttccgcagat aaaaatggaa
     3721 ctgtggcagc ttcggaagta gcaccgtcct ctcgttggaa taaggtggaa tttcagaaag
     3781 acggtgcact gtctgacacg ccggatctga cggacgacac ggtatacatg gacgagtatg
     3841 tcaattatct gatcaataat ctgggggatt ctacaacttc taccggtatt cagggctata
     3901 atctggacaa tgaaccggta ctctggaatg atacacaccc cctgctgcac aaggacgaag
     3961 tttccaacag cgaactgatc tccaagtcgg ttgctctggc aaaggtggta aaggatcttg
     4021 atccgaacgc agaaatttac ggtccggcat tctggggcat tctcccctgc gtacaggcag
     4081 gaagcggcga caacttcaag gattcggatt gggaagctgt caaaggacag tattcgtggt
     4141 atatggatta ctacctgaaa caaatggcag atgcagagca ggaaaacggc aaacgactgt
     4201 tagatgtagt tgatgttcac tactatgcac aggactgcga aacagatgac ggcattttgc
     4261 aggcagcgag aagcctgtac gatccgaatt acaaggaaaa cagctggctg acacagtggt
     4321 ttgggggttc gttcccgttc ctcaccagaa tgcaggagtc tattgacaag tactatccgg
     4381 gtaccaagct ggcactgacg gagtacaatc tggcaaacat cagcaaggaa tctacaactg
     4441 gaaaatctgt aatttctgcc attgcagaaa ctgaagcgtt gggggctttt gccgatcagg
     4501 gcgtatatct ggcaacctat tggggaacgc tttctctttg tccgtatgtg accagtgcga
     4561 tcaaccttta cactaactat gacggcaatg gcggtgcatt cggggatacc cttgtggagt
     4621 cttcctctgc tgacctgtct aaggcggcaa tctttgcggc aattgatggc tctgatgaca
     4681 gtgaagtgac tacagtgatt tccaacaagg ataaggaaaa tacagaaaaa gcggttattt
     4741 ctctggaagg cacagacact gactatcaga gtgctgttgt ctatgctatc acacaggaca
     4801 gcagcgatat tcagattctg gatgtgcaga atgatgtttc cggcaatcag gtaactgtag
     4861 aactgccgcc gttgtctgtg gctcaagtgg tgatttctga cgaaaagacc aatgtgacaa
     4921 ttccggaaaa accgaatgtt gaaatcaaaa aaaccactta caatatcagc gatttgtcga
     4981 caaataccgc aggcaatccg atgattccgc tgggtgacaa ggaacacctg aaagaaatta
     5041 ttgtgaacgc tactgtatct tcgaatgccg gatcttcgtg ggctgtcggc ggcggagctc
     5101 tctgctttaa cgaagtcgtg aaaaagggtt cctcctctgc tggcgtttgg ggaaacaagt
     5161 ctttccaata ccgtctgggc acaagtgata taacagttcc ttttgacgat cagtttacta
     5221 tttctttgag tgacggcaag agtgaaaata ttactggttc ctgcaatgat gaaagtgcag
     5281 aattacagaa ctggtgggct tcttccgaaa acgattccaa aaacggcgaa gatatctcta
     5341 ctacatttaa tacagtgaca ttggtgtatg aatacaatta tgacaaaccg gacgaaacga
     5401 ctacaacgcc ggcagaaaca accacaacaa cagaaacaac gatgacagaa tccacttcgg
     5461 aaactgtttc tgaaaccact gcttctacag aagaaacagc attgactaca gagagtaccg
     5521 ctgcttcgac ggaaagcagc aacaccactg ctgtgtcctc ggaagaaaca accacgactg
     5581 caacagaaac tacctctgct gcaacttctt ctgaacaggt gaacccgtcc gcagttttgt
     5641 atggcgatgt tacgctggat ggcagagtgg atattactga tgcggtgctg ctgaacaagg
     5701 caactgctgg tcaggtagaa ctgaatgtac aggcaaaggg caatgctgac tgcaacgctg
     5761 atggacagac ggacagcaat gatgcaattt gcctgctgcg gttcttggtg catatcatca
     5821 acacgttgcc ggaacagtaa gcctctgcaa atatttatga caacgaaagc cactccgatt
     5881 ttggagtggc ttttttgttt ggattcgttt aacaatattt acagaagcga aagctgtccg
     5941 ctttccggca gatctttcgc catgccacca gttgccggaa tggtttttac acgatatttt
     6001 ttcagtgcaa tgcattcttc ttcgttcagc acacgtgctt cgcagacaag gtgttttgct
     6061 ttcaatgtga ctacctgtac gccctgtgta tcacgggtgg actttgcgga aatcagtaca
     6121 gtatctgcca gcactgcccg attgccgtct gtacggaaaa agacggagca ggattcctcg
     6181 ccgcttggaa tttgcagaat ttgtaccagt ggcgaacggt cgctgtaagc cttttgcagc
     6241 ttcttacggt tggttttcgt tgcgtaggca gacatcggta cacgggccgc ctttccgttg
     6301 gcaaatacaa agagcagatc accttggtaa tctgcggtgg caatcatttg caccacgaat
     6361 tccccgtcag caaatcccag ttttgccgga atgtaatcgc ccatgacgct ggctttggtt
     6421 tccgtaaagt cgctggctct ggttttgtaa acctgctgtt ggttggtgaa aaacagcagt
     6481 tcgatgcagt tggtggtttc cagatgacag acgatctggt cttcctcttt cagcttttgt
     6541 tcgctgctca tgcggagaga agctggtgtg atctttttga agtaaccact ctgcgacaga
     6601 aacaggtgta ccggataatc gggaatatct tcttcgtctg gttcttcctc gatgtcagac
     6661 gcataataga acagtgtctt tcggggctgt ccgtattttt ttgcggtttc tttcaactct
     6721 gcaatgatga ttttcttcat ctttttcgga tccgcaatgg tttcttccag ttcggcaatt
     6781 tcctgtttca actggtcgat ctcctgcgtg cgtttcagaa tgtattcccg attcaagtgc
     6841 cgcagtttga tctccgccac atattctgcc tgaatctcat caatgccgaa gccgatcatc
     6901 agatttggta caacatctgc ttcttcgacg gtttcccgca cgatgcgaat tgccttatca
     6961 atgtccagca ggattttttc cagaccaagg agcagatgca gtttttcctg ttttttctgg
     7021 agatcgaata ccagacggcg gcggacacat tccagacgga atgcggacca ttcctccaga
     7081 atttctcgca cgcccataac gcggggtgct ccgccgacca gaatattgaa gttgcaggag
     7141 tagttgtctt gcagcggtgt catgcggaac agcttctgca tgaccttttc cggatcagag
     7201 ccacgcttta agtcaatggc aatcttcaag ccgtcaatgt cggtttcgtc gcggacgtaa
     7261 gaaacttccc gtagtttgcc ctgtttgatg cagtccacga tcttgtcctt gattgcttca
     7321 atggtggtgc tgtacgggat ttctgtgact tccagacagt tgtcctgttt gttgtagttc
     7381 cattttgcac ggacagttac actgccgcga ccggtatcat aaattttttg cagttctgtt
     7441 tcattgtaga acagaaagcc gccgccggga aaatccggtc ctttcagtgt agacagaata
     7501 tcatgcttcg gatttttcag cacggcaatg gttgtttcgc agacttctgc cagattgaac
     7561 gggcagacgc tgcttgccat ggaaacggca atacctacat tggcattgac cagtacggtg
     7621 ggaaatgttg ccggaaacag tgtcggctcc tgcatggtat tgtcgtagtt cggaacaaag
     7681 tctactgtgt ctttgtcaat atcccgaaac agttcgttac aaatcggttc cagtttgact
     7741 tccgtataac gggaggcggc gtatgccata tcttttgaat atgccttacc gaagtttccc
     7801 ttggattgca cgtaggggtg cagcagtgcc tcgttgcctc ttgccagacg caccatggtc
     7861 tcatagatgg cactgtcacc gtggggattc agccgcatgg tctgccctac aacgtttgcc
     7921 gatttggtca gcccgccggt gagcagtccc attttataca tggtgtacag cagtttccgg
     7981 tgagaaggct tgaagccgtc gatctccggc agtgcacggg aaatgataac gctcatggcg
     8041 tagggcatat agtttttttc cagtgtatct gtaatttttt cttcttccac aatgccggca
     8101 ccggcaatat aggcattggg aattgcattt ttcttaggtt tttcagattg cttttttcgt
     8161 gccatttttg ccctcgcttt tatgaaatgt ccgccagttc cagatactcg ctaccgtatt
     8221 ctgcaatgaa gtcttttcgc ccctgcaggt catcgcccag cagaatgtcg aacatctttg
     8281 ctgtttcttc gatgcctgcc gcagtcacct gaatcagccg gcgtgtttcc ggattcatgg
     8341 ttgtcagagc catcatgtct gcttcgttct cgcccagacc ttttgaacgc tggagagtgt
     8401 acttctcccc ttccagtgtt gctagaatct ctgttttttc cttttcggta taggcaaagt
     8461 atgtccgctt tttggttgtg atttcaaaca gcggagattc tgcgatatac acatagcctt
     8521 tgtcgatcag tgtcggtgtc agacgataca gcatagtgag aatcagtgtt cgaatctgga
     8581 aaccgtctac atcggcatcg gtacagatga caattttatt ccaacgaaga ccttccagat
     8641 ggaaagagga aatatccttg tttgccttgg tagtaacttc taccccgcag cccagcagct
     8701 tgacaatatc agtaatgatc tcgtttttga agatcttgtt gtagtctgct ttcaggcagt
     8761 tcaggatttt gccacgaacc ggaatgactg cctgaaattc tgcatctcgt gccattttta
     8821 cggaacccaa tgcagagtca ccctccacga tgtacaattc ccgacgggac agatctttgc
     8881 tccggcagtc tacaaatttc gggatcatgt tggtgacgtc aatgctgccg gtcagctttt
     8941 tcttgaggtt cagacgggtc ttttcggcat tttcacggct gcgtttattg atcagcacct
     9001 gtgctgcaat tttttctgct tcgtccggat tttcaataaa gtagatctcc agctgatgct
     9061 tgaggaattc cgtcatggct tcgtagataa atttattggt gatcgccttt ttggtttggt
     9121 tctcatagct ggtttctgtg gaaaaactgg aagaaatcag caccaggctg gattccacgt
     9181 cctcaaattt cagtttggct tcacttttca ggtatttgtt attctgtcgc agataggtat
     9241 cgatctggga taccagtgcc tgccgcacag ctttatccgg cgagccgccg tgttccagcc
     9301 agctggaatt gtgatagtat tccagtcgct gtacctgatt ggaaaaagtc agagccacat
     9361 tcagcttcac cttataggtc ggcttatcct cacggtcacg gccctctcgc tggcagctcc
     9421 agttctgtac ggaagtcagt gtgttttctc cggcgatttc ccgcacatag tctgcaatac
     9481 cattttcgta gagatactgt cgttcggtaa aagtgccgtc agcttcttct tttcggtaga
     9541 ggaacaacag gttcgggttg acaactgcct gccgccgcag agtgtcacag aaataatcat
     9601 ctggtatttc gatctctgta aaaacgtcca gatccggcag ccagtgagtt cgtgtgccag
     9661 tctgcttctt cttggtcggc ttcttttgca gtccgcctac attgacaccc ttttcgaaat
     9721 gcaggttgta ctgaaagccg tcacgaatga tctctacgtc catgtaagag gaagcgaact
     9781 gtgtggcaca aagtcccaga ccgttcagtc ccagagaata cgcgtagttg tcatcattgg
     9841 agttgtattt accgcctgca tagagttcac agtagaccag ttcccagtta tagcggttct
     9901 cgtttttatt gaaatctacc ggacagcctc tgccgaagtc ttccacttca atggaattgt
     9961 cctgatagcg tgtcacgacg atcttgctgc cgtatccggc acgtgcttca tcgatagagt
    10021 tggaaatgat ttcaaaaacc gaatgggcac agccgtccag tccgtcagag ccaaaaatga
    10081 caccggggcg ttttcgtacc ttgtctggtc ctttgagcat cgtgatactt tcattatcat
    10141 aagattgctt tttcgccatg ttcgttcctt tctaccggaa aagttccggc tttttcagcg
    10201 gtttttctgc atgaaaaagt ttccgcttgt agtgcattat ttctatcata ccacaaaact
    10261 aacggaaaat caagcttttt ctgaaatttt gaaagaatca gcataagaac tctcacagaa
    10321 ttgccggatt tttttgtggt tctgcctagg aatttcattt ggacagtatt ctgtcagctt
    10381 tatg
//
LOCUS       DAWBGR010000082        10306 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1875_length_10306_cov_4.183689, whole genome shotgun sequence.
ACCESSION   DAWBGR010000082 DAWBGR010000000
VERSION     DAWBGR010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10306)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10306
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1875_length_10306_cov_4.183689"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8978..8987
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2409 a         2485 c         2897 g         2505 t
ORIGIN      
        1 ctgacgaaaa atttgacgac gcatctgtcg cacaatcttt gtgcggtatt gccttgacaa
       61 tttccgccag ttatggtata ctgttctaag ttagaaaaag ctaaccatcg ggaagcacca
      121 catgtgatcc catgcagtgc ttcccgataa tcgtacatgc aggttagcga aaactaattt
      181 ccaaacaaag ttttttcaaa aagaggacgg tgtaacatgt ttttagagat tcaacaaatc
      241 aagaaacatt tcggagaggg tgaaagccgt gtcgatgtgc tcaggggcat tgacatcagc
      301 attgaaaaag gcgaaatttg tgtattgctg ggaccttccg gttccggaaa atccacgctg
      361 ctgaacatca tcggcggcat tgaaacgccg gacagcggca agatcaccat tcacggggaa
      421 accattgggg aaatgaacga aaagaatctg acgctgtacc gccggaagca tctggggtat
      481 attttccaga tgtacaatct gatcccgaac ctgaatatca aggagaatgt ggaagtcggt
      541 gcgtacctga gcgacaatcc gctggacgta gacgacctgc tgaaaacgct ggggctgtat
      601 gagcaccgcc acaagctgcc gaatcagctt tccggcggac agcagcagcg gacggcgatc
      661 ggcagagccg ttgtcaagaa tccggacatt ctgctgtgcg acgagccgac cggtgcactg
      721 gactaccaca cttccaagga gatcctgaag ctgctggaag atgtcagccg caagtacggc
      781 aatacagttg tcatggttac ccataacgac gctctgcaaa atatggcaga ccgtgtggtg
      841 aagctgcgtg acggaaagat ccgtaagaat tttgtcaacg aaaccaaaat tccggcagca
      901 gaattggagt ggtgaccgat gacgaaaaag atgaaaaatc cattgaaccg acggctgccg
      961 cgggaactga aaagcgaatt cggaaagtat ctggtgatct ttctgttttt tatcatggtg
     1021 atctctctgg tttccggttt tctggtggca gataccagtc tgcatacggc ttatctggac
     1081 gctttcgaca agtacaatat cgaagacggt aactttgcgt atgtggaaga agcttctgac
     1141 gatgccattg ccgcaatcga aaaagacggc aaggtcaagg tctatgccaa cttctacaaa
     1201 gaagaagaca cggacgattt cgaaagcaca ttccgtgcgt tcggagaacg aaacgacatc
     1261 gacaaggtgt gcctgctgga cggcgatatg cccaaggcgg acactgacgt ggcaattgac
     1321 cggctctatg ccaagaacca tgatctggaa attggcgaca agttcggcgt tgccggaaaa
     1381 gacctgacca tttccggcat tgtggctctc tcggattatt ccacactgta ccagaatgcc
     1441 tcggatatga tgttcgataa cgacaagttc ggtgtcggcg tcatgacaga tgcagggttt
     1501 gcagccttgc gggaaaacca cctgcactat aactacagct ggagttataa cactgcaccg
     1561 gaggacgatg ccgaagccaa ggacatggcg gaagatctga tgccgattct ggcagagaat
     1621 ggcatgctga ccaatttcct gccggagtat ctcaatcagg cgattatttt tgccggcgat
     1681 gatatgggcg gcgatatgtc catgattctg gcatttctgt atatcgtcat tgtgattctg
     1741 gcgttcgtgt tttccattac catttccaac aacatcacca aggaagcgaa tgtcattggc
     1801 acgcttcgtg cttccggtta ttctcgcgga gaaatggtgc ggcattatct gatcatgccg
     1861 atgctggtgc tgctggttgc cgcagttgtg ggaaatgtgc tggggtacac atggctggaa
     1921 cagatattcg ccaaccagta cctcgcaagc tacggcttga ccacttacga agttctgctc
     1981 aattcgcagg catttgtgct gacgacagtg atcccgctga tcctgctgtt catcatcaat
     2041 tttgtgatgc tgactgtcaa gatgcgaatc tcgccgctgc aattcattcg ccgcgacctg
     2101 tccaagcgga agaagaaaaa ggcattccgc ctgaatacca aaattccgat catgcgtcgc
     2161 tttcagatgc gtgtgatttt ccagaacttt ccgaaccatg tcatgatctt tttcggcgta
     2221 ttctttgcca acttcatcct catgttcgga atgatcctac agccgatgct ggtgcattat
     2281 cagaatacca ttaccgacaa tgtgctttct gcatatcagt atattctgaa aacaccggca
     2341 gaaacagaaa ccgacggggc agaaaaattc tgcttcggca gtctggacac cttgccggag
     2401 aaacgcagca gtgaaagcgt ttccctgtac ggcgtaaagc cggacagcga ctatgtcaag
     2461 ctgcactctt ccggaaaaaa agtagatatt tccactgcct atgcagaaaa gtacggcgtg
     2521 gaaaagggcg acaccattac tctgaaagac ccctatggca gcgacaagta caagtttgag
     2581 gtcggcggca tctatgatta tccgtccacc attgccgttt ttatggaaca ggatctgttc
     2641 cgtcagacgt tcgattacga cagcgattac tttaacgcct atttctctga ccagaagatc
     2701 aaggacattg acgatgctct gatttccaca gagatcacag tagacgatct gacaaagact
     2761 tcccgtcagc tgattcgctc tatgggcagc atgatgaatt tgttcttggt attcggtgca
     2821 ctgatgttcg tgctgatcct ctatctgctg tcgaagatca tcatcgaaaa gaatgcccag
     2881 tcgatctcta tggtcaaaat tctggggtac aacaatcggg agatcaaccg catctacctg
     2941 cactccacga cgatcgtggt aatactgtgt attctcatta ccataccgat ttccagtctg
     3001 gtgatgaaag aagtcgtaga agtggtattc tttgaatact ccggctggat cacctattat
     3061 atgccggcag tcacttacgt ggaagttgcc gcatcaggtg tgatctgcta tgcaatcgtt
     3121 gcgttcctgc tgaatcggaa ggtgaaaaag attccgatga gtgacgcact gaaaaatgtg
     3181 gaataaacta taactcacca atcaaaaaag caataccgca aggggaaaac tcctcctgcg
     3241 gtattgcttt ttcttgctta ttataaaccg gaaatcaccg ccgctgctgt ttccgcatgc
     3301 ggtagtcaga cggtgtacag ccgattgctt tgcggaatgt ttctgtgaaa tagctggagc
     3361 cgctgaatcc gcactgcatg gcgatttccg tcacgctcag atccggattg gacagcagtt
     3421 cgatgctctt ggaaatgcgg tattccgtca gataagcatt gggtgtctgg ttcaggtatt
     3481 ccttgaaaat ggagcagcag ctgctgcggc agacgtaccc tgctgcggcg atgtcctgta
     3541 atgcgatttt gctgccgtac ttctgctgga taaagcctac cattttgtgc atggcttcca
     3601 gccgcttgct gtcgttgggt tcccagggtt cggtgttgtt ctgtgtcaga ttccacaggg
     3661 cgtgacagat gcggtagatg cagctgaaca gcagcagcgt gtatccctcc tgctgaacag
     3721 atgcacactg aaacagttcg cagacatcgg tgatgatcgc cttttccgcg gcgatttcag
     3781 ggtgcaggat cagatatggc ggagcatggt ggatcaccgc gtccagcagg tctttggtaa
     3841 cgggattcgt tgcaagcgtc ggcgggaaca ggacacagtc ataaacccag ttgctggatt
     3901 tttcccagta tccatagtgc atctgtccgg aatttacaaa gatcatctgc cccggcagca
     3961 gcctgcactg ttcgccattg acgctgtacc acatgggagc gtttttgacg tagatgaact
     4021 caaattcggt atgccagtga ttgacaatgg agagtgaggg atagtcctcc tgtgaaaaat
     4081 gggaagcttt gatcggcaga ttcggaacat tgtattggat aatttccgat ttgttgtctg
     4141 tcagttccag tgcactgtac atgtggaagg ctcctttcat ctctttgcta aggaaacact
     4201 gaataaagcc gtcaggcggg ctttgtgcgg tattgcccta attcttcacg cggctgcctg
     4261 tgtcagggct tccggctctg ccgccggttc ctgtcgtgtc agattcttta cccagttgtg
     4321 cttgcggtag ttcttgagca ctgccggaag cttgatgatc tcgtctacgt tgatcagaat
     4381 ataaacggtc agtaccggca gtttcagaac gaacgccgca atcagaccaa tgggaacggt
     4441 gacacaccac atggtgatgc cgtcacagat aaagccgaat ttggaatcgc cgccggctgc
     4501 aaagatgccg gagatcactg tcatgttgat agactttccg atgacatagt aggagcagac
     4561 caccagcatc acacggagat agcccttggc ggtgtctgtc agagtggaga acagcggcgt
     4621 aaaggggatc acacccagaa taatcacacc ggcgatcaca ccagcaaaaa tagaagtctt
     4681 ggaaagcttt ctgccgtatt cctttgcctt ttccagttcg ccctgtccca gcaagcttcc
     4741 caccataatg ccgcttgcgg tggcgatgcc ggtgcagaag cagatgacca gattcttgac
     4801 gatgttggca atggagtttg cggctgcggc atcgctgccc atgtgtccca taatgacgga
     4861 gtacatggtg aagcccagtc cccacatcag ttcgtcaccg aggatcgggg cagtgtattt
     4921 ccagaagtcc cgcagcagca ccttgtccgg acgaatcgcc cagcccagcc ggatcttcac
     4981 gctgtccttc ttcatcatga ccagcagtgc ccatgccagt tcaatgacct tggcgataac
     5041 tgttgcaatg gcagcaccgg cgatacccat tttcgggaaa aagccgatac cgaaaataaa
     5101 agctgcattc agaatgatat tgatgactac ggaaacagaa ctgatgactg cactcagagc
     5161 ggcgttgccg cagttcttca gaatgcacag gtagatctgc gaaacactga gcagcaaata
     5221 ggaaacgccc actacccgca gatattccgc accgtctgcg atcagcacag gatccgatgc
     5281 aaatgcccgc atcagctgtt ccggaaagaa cgttgcacca atggtgaacg gcagagacag
     5341 cagaacggag aatgccagcc caatgcccag cgtcttttcc acggcacgct tgttgcggat
     5401 cccccagtac tgtgcggcga aaatggaaac gccggcagta atggcaaaca agaacaggga
     5461 atatacgaac tggatctgtc cggcgaggga tactgccgag agagcgtcct gactgacaaa
     5521 acccagcata aatgcgtctg aggcattgac cagtgccatc atcagttgct ggagagagat
     5581 cgggatcatc agatgcagca gttttttccg gaattctgca cgattgcttt gtgtcatatg
     5641 tcattctcct tttctgtgat ttttttcaaa caaattcagg tgtatttccg gagcagagca
     5701 acacgtcttg ttccggattc gtttgatatt ataagacaaa gaaaattcga tttctataac
     5761 attccgctga atcattataa caatcgtaca aattcaaggg atttgaaaaa cgttttgcag
     5821 aatatgtggt atcgatttca aaatcgtgca caaattgtac ggttttgatg ttgctccgat
     5881 catatcataa aaatatctgc ttgtcaagct gccggagaaa aaagaatttt ttacaagacc
     5941 agtatggcac ttgaacaaca tcaacagata tgatataata aaagtgtacc caatctgtga
     6001 aaggagcatt tgcacatgca gagaacgatc ccacggctgc tgatcagtgc agtcggcagc
     6061 ggcaccggaa aaactacagt taccgcagca ctgctgtctg cactctgtgc ccgcggcatg
     6121 cgtgtgcaga gcctgaagtg cggaccggac tatatcgacc ccatgtttca tagtcacatt
     6181 accggaagac cggcatacca tgccgatcct ttttttctgt cccgttttgc catgcgggaa
     6241 ctggtttccc gtgtcagtgc agatgcggac tgcacgatct tcgagggggc aatgggctat
     6301 tatgacggca tcggcggcac ggagcaggcc agtgcctaca ccgtttcgga ctggctggaa
     6361 ctccccacgc tgctggtggt aaatccccaa ggtatgggct gttccatagc ggcagtgtgc
     6421 cgcggattcc agacgttccg gacaccgaac ccgatctgcg gcattttgct gaacggggtt
     6481 cgccgcggca tggctgccta ttatcgggag attctggaac gggaaaccgg cttgccggtg
     6541 ctgggctgtc tgccgcagct ttcggaggtg cagctggaaa gccgtcacct tgggctgatg
     6601 acagcgggag aggtgtcccg actggacgaa aaagttcggc ttctgggaaa gactgcggaa
     6661 gaaaccatgc agctggatca gattctggaa ctcgcacgca aggcaccgcc gctgccggaa
     6721 gtgccgctgc tgtcaaagcc gcagccgtca tttcggctgg gcattgcaca ggatcaggca
     6781 ttctgctttt actatgcgga aaatctggaa ctgctggagc attatggtgc agaattggtg
     6841 ccgttttcgc cgctggaaga tgcagccctg ccggaaaatc tggacggctt gtatttcggc
     6901 ggcggttatc cggaattgta cctgccccag cttagcggca acacgttgtt tctggaatcc
     6961 ctgcggcagg cagccgaaac tggtatcccg atctggggag aatgcggcgg ctttctgtac
     7021 ttgcagcagt ctatggctgg aaaagacggc atacgctatc ccatggctgg actgctgtcc
     7081 ggagatgcgg cactgggaga acgcctttgc cagttcgggt atgtcacgct gacggctcgg
     7141 caggacaccg tgttcggaaa ggcaggagca tcaattccgg ctcatgagtt ccattatgcc
     7201 gacagcacgg cgaacggcaa cgcatttctg gtgcagcgtc ccaatgggaa aacgtgggag
     7261 gcaatgcagt gtcggggaaa catcattggc ggattcccgc acttgtattt tcccgccaat
     7321 ccggaatttc ccatgcagtt tgccgcggct tgtcgggcgt atcggaagaa aagaggtggc
     7381 atatgctgat tctggtaaca ggcggtgccg gcagcggaaa gtccctgctg gcggagcgta
     7441 tcagcatgca gctgaaacgc atgccgttct ggtatgtggc aacgatgcag gtatgggatc
     7501 aggagtgccg cgaccgcatc gaccgccacc ggcggcagcg tgctggaaag ggctttcaga
     7561 cagtggaagc ccctgtgcat ctggcagaag cggcagcagc actgccgccg gacggaacag
     7621 cactgctgga atgtttgagt aatctggtgg caaatgagca gttcggcggc gacaccggag
     7681 caccggtgca ggcggttctg gacggggtgc atgctctgta ccggaatgtg cggcatctgg
     7741 taattgtgac caatgaggtg ttttctgacg gcacgccggc tgattctgcc atgcaggcat
     7801 acctgcaaaa tctgggaaac atcaactgtg ccctcggggc ggaggcagac gttgtggcag
     7861 aatgctgtgc cggtctgccg gtgatctgga aaggagaatc gttatatcat gaaatcatgg
     7921 ctggaatctt tgctgatcgc actttccatg tatagtgcga ttccggtaaa gtgtttgaac
     7981 tggactgaga aaaatctgcg gaatgcaatg gtgtttttcc cgttggtggg gctgctctgc
     8041 ggggtagggc tgtggctggt ctggctggtc tgcggcttgc tgcatgtcag cagtgtgctg
     8101 tttggcgtgc tggcagtggc aagtggtgtg ctgatcaccg gcggcattca tctggacggc
     8161 ttctgtgata cggcagatgc tctctattcc cgccgtccgc aggaggaaaa actgcggatt
     8221 ctgaaagacc ccaactgtgg accttttgcg gtgttcagcg taatactggt gctgttggtc
     8281 agcttcggtt cgtatgtgca gctgtatcag acacaggggc ggaatgtggt ctggctgctg
     8341 gcaggcggat ttttgctgac acgctgtctg agcgggattg ccgttacgac gctgcccgtt
     8401 gccccgacca gttcgctggt gaaaacattc ggggaaaatg ccggcaaata tgttcgtgtg
     8461 ctgctgctta tcgagtacgg cattaccggt ctgctgctgg tgtggttctg gggctggctg
     8521 gcagtggtgc tggtctggct gggactgttc gtcttttggg gctgttctca catggcgaag
     8581 aagcagtttg gcggtatcac cggcgatctg gcaggctttt tcctggtgct gagtgaaacc
     8641 gtgtacctgt tgggagcagc tctgctggga ggtgttctgg cgtgaaactg attctcggcg
     8701 gctatgccaa cggcagaact gcctacgcca tgcagaacta tcagctgaca gaatcggact
     8761 gttttgatgc ggcggcagaa ccgcttgccc ggtggaacgg gcagcggctg atcctgcatg
     8821 cggagcagct ggtgaatccg tggctggagc agggaaagga accgtgtacg gaaattttgc
     8881 catggctgga acggtggcag aatgcagtgc tcatcacgca ggaagtgggc tgcggtctgg
     8941 tgcctgtcac cccgcagcag cggcagctgc gggaagcnnn nnnnnnntgg gacatttcaa
     9001 cagattgttg gcagaatgtg cagaaacggt ggaacgtgtc tgctgcggtc tgggcatgca
     9061 gctgaaatag gagcagaacc atgtggatat tgttgagtgt tgcgttggga tttttgctgg
     9121 atctctgctt cggggatccg gtctggctgc cgcaccctgt ggtgctgatc gggaaaagca
     9181 tttcgcggct ggaaaaattt ctccggcaaa agttcccgca aacgccggca ggggaaacaa
     9241 aggcaggcgt gattctggcg gtctgcattc ccctgtgcag cctgctgttg tccggcggaa
     9301 tcctgctgct ggcgtatcat atttcgttct ggctctggtt tgtgctgcac acgttctggt
     9361 cgtatcagat tctggcagca cgttgtctgg agcaggagag ccggaaagtg tatcggtttc
     9421 tggcggctgg cgatttgtcc ggtgccagaa cgcagctttc ctatctggta ggacgggaaa
     9481 cgtcacagct ttctgctgaa gaagtgacga aagcctgtgt ggaaacggtt gcggaaaata
     9541 cctctgacgg cgtgactgca ccgctgtttt acttgctgct gggcggcgtg ccgctggggt
     9601 tttgctataa ggcaatcaat acgctggatt ccatggtggg ctaccgcaat gcaaagtacc
     9661 tctttttcgg cagggcatcg gcaaaaacag acgatgcggt aaactggctg cctgcccgta
     9721 tctgcggtgt gctgctgact gtttccgcgt ttcttctggg actggacgga aagggagcat
     9781 ggcggatttt tcgccgtgac cgaaacaacc acctttcccc gaactctgca cagacagagt
     9841 ccgcggcagc gggagcactg ggcattcagc tgggcggcac ccatgtctat ttcggggaag
     9901 tggtggaaaa gccaacgatc ggaaatgcag acagaccggc agaaccggag gacattcccc
     9961 gtaccaatgg catgatgctg gcggcatcgg ggctgtcact gctgggattt ggtctgctgc
    10021 ggctggttgt agttttgctg ctgcggtaag tgaaaaccga gtttgccatg tatgccatcc
    10081 atgaaaaagg gtggagaggt tctcctgtga agtgtagctt ctagggcagt cgtgttgaaa
    10141 ccgtccggcg gaattcgctc gttcctcgct gtctgcctgt ggctttccaa cgccgattgt
    10201 tgcccttgcc tgtattggca gtgttgatat tctggtgttt tctggtgcag gcggtcaact
    10261 ttatttgata cggcgtttgc aagtttatct gtttatcatc tttcat
//
LOCUS       DAWBGR010000083        10304 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1876_length_10304_cov_4.950532, whole genome shotgun sequence.
ACCESSION   DAWBGR010000083 DAWBGR010000000
VERSION     DAWBGR010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10304)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10304)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10304
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1876_length_10304_cov_4.950532"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2306 a         2660 c         2464 g         2874 t
ORIGIN      
        1 tccggtgact tcctgctttc cggtgatgtc cttcttggca atggtaagct tggaagtgtc
       61 atcttccatt acgatcttgt tgtcgtcctt ggtcagtccg tttaccgttc cgaccttgcc
      121 gtcagctccg acctcgaagt tcatcgcctc agtctttttg gtgaagccgt ccggtgcggt
      181 ggtttcttcc agcgtgtaca cgcctgccgg cagatttgcc agtgtcacac cgcccttggc
      241 gttgtccgaa gtccatgtaa tggtgttgcc gctggtttcg attccagcaa tgctgccgtc
      301 gctgtaggag cccttgactt tgctcaggtc tgttcctgcc ggtgcagtca gtttcagtac
      361 ggctccggtg agttcttctc cgccgccgac tgcctgcttg ctgatcgtca cgctgggctt
      421 ctgaccgtcc ttcatggtaa tgtcgttgcc agaaacgtca tattctgtgg ttcccgttgc
      481 atcaactaca ccgtttttaa tggtaaaggt gaagtctgtg cttaccttgt tgtagccgtc
      541 cggtgcaact acctccgtca tggtgtaggt accgtccggc agaccgatga tgtcggtctt
      601 gtttttgccg tcagaaataa aggtcagttc cttgctggtc agctggtact gagattcgtc
      661 gtccacgctg tccgacttgg tgaaggtggt tcccgttctg gagagttcta ccagagcttc
      721 gttccagctg ccgtctgtct tggtaatggt gatctttgca ccagccagtt ccttcagaga
      781 ggtgatgtct ttcttgctga cgctgatctg cgaccggttg atgacagtca gtccgttttc
      841 cgtcagtgag aaattgctgc cggtgatcgg gttgccctgt gcatctgtgg tgacaatata
      901 accgtttgca ttgactgcaa aggaaatgct taccggtgca tagcctgtca tgggcttttc
      961 tgtcagggta tattcaccct ctgccagtcc tgtgagtctc tccggatctg catcagatgt
     1021 ccatgtcttg ctgtagccgc caggaccggt cagtgtcatt tcacagcctt tcataacgct
     1081 gcccttgaca tctgtttttg caaaggacaa ttccacttgt gtggtattgg catcagtaat
     1141 gaccttttcg cccggaatac ccggcagcag gtctacccga ctgctgttgc cggagtaggt
     1201 agcgaggaat gtcttgctgc tgcctgccga tcctactgtg gttgtgctgc cattttccac
     1261 gcgtttgcct tctgcgtcca gctcaaagac gtaataggtc ttgctgctgt ccaatccgtc
     1321 aaaggtaaca gactttttgc ccttgtcgcc ggcagcaatg ttgatgttct tcttttccac
     1381 ctgctgatag ctgccatttg tgtcctgcgt aaacagtcca acttcaattg catggtcaac
     1441 tgccggattg ccgtcctcgg tagcaatctt cgttaccttg atctgagccg gattcttggt
     1501 ggtgtttgtg ataatggtgt ttgcctgcgg tgcgtccttg gtcagggtaa gtgccgcaac
     1561 ctgattgtgg tcatcacagc agtcataaga aacgtagaat gtcgtatttg ttttcgtagt
     1621 ggaattcttg ggatcgagaa caacaggcat ctggaaagaa gcaccgtttt cgacacgctt
     1681 gtcaccgtcc atttcaaaaa tgtagtacgg gataccttct ttcaaatcgt accgtttgtt
     1741 ctgttcattc agttcagtac tgaatttcac ctcagaaccg gcagcgactt tttgagttgt
     1801 gccgacctgt gtgtaatcgt ttccgttctt ggtgaacagt cccacggaga atacgggtgt
     1861 gctcttgaca tcatctgcga tcagcacctt gctgacggtg atgctgtatt gggtttgttc
     1921 aaagttagtg attgtagtag taccgcctgc ctgtactgcc gaccgtgtta ccggtaagaa
     1981 agagtagcgg ataccactat ccgcagtgta agtaccagaa tctcctgtat aagtcggaac
     2041 gccgttggca ttggtttcca gcacgtagaa atccataccc ttgggcacgt tttcaaatgt
     2101 aactttcttt gagtctgcct cggtaaaggt cagggactgc accggtgctt cctcatagcg
     2161 aacataatcg cctgccgcat tcttggtgaa cagggcaaaa tagaaagtat caccggtgac
     2221 gtctccgcct tccacgcctg ccagagtttt ggtcagtgtg atctcacctg tatttttggt
     2281 attggtcacg ccgacagtct ggtccgttgt ggcatcagtg gagatctcat tgccggtata
     2341 tgccggtgtg tagttgcgaa tctgattgcc cttggcatct gtttccagca ggtaaacgtt
     2401 gttggcgttg ttggtctgct gcttgatcga ggacaggtct acgtccttca tggtgacaga
     2461 agccgcggtt tgtcctgccg gaatggtgac ggaaatcaca tcactcaacc gtgtggtgta
     2521 attttcatcg gtaaacagtg caaagtagaa cgttgcgtcc ttggtttctg cttctgtcaa
     2581 ggtcttgttg accttcacat caacggtggt gtccggtgtc tgtacccaga cgccgacctt
     2641 tgccggctct gctaccatga cagtatcgcc cagaatgtca ggattctgat aggagtacgc
     2701 gaagctgttc catgcgatgt tttcctcgcc ggtctttgcc acatagctgg gaacggttgc
     2761 ggtaactttg acaacaactt catcgccaac cttgacggtc gtatcttgca gctggatccg
     2821 gaagtctacg gtatcgtcag acttgatgtt tgtccagttc atgacatcgt tctgccccag
     2881 ccagtccttg gagtactcat tcaggacaga ggtcttgtcg gtggaatagc tacgtgctgt
     2941 tttatcaatt acatctgttc cgcctaccat aacagaatca atgctcttga gtttcacgcc
     3001 gaatgcggag aaacgctcat agccggaaac cagacccaag tcacccacat agggcagacg
     3061 gtcaataatg gtcatgtttt ccagagctac cggcgagttg ttcttgatgt ggagttcata
     3121 ggtcacatct tcgccctgca tgccctgcac ataattgtgg gtgggctggc ggctgtaacc
     3181 ggtatcggta gccggatccg tatggggtgt gccggtgtga ttctgattgg tgtaggtaat
     3241 ggtcttccac gattcggtgg tcagaccaac gatattgtaa tttgcatagt tccagatctg
     3301 cgtgtcgcgc ttttcgccgg caaccgtatc ctctgcggtg aacggctggt caatggtggc
     3361 gtaaccggtg ttggtgataa cgccttcttt ttcctggccg tcttttacag tggtggcgaa
     3421 ttgtacgacg atggcatagt tcggctccag ttttccttct gtaacgcctg ttcctgtcac
     3481 actggaaaca tcccagctga cagaatcacc gttagcagtg gttttgattg ttgcaccatt
     3541 tccgctaacc ataccatatt tgtccacagc aaagctgccg tctgccttca gcggcactac
     3601 aaaacatttt gtagggtagc tggtgttgtc aaattcatag cagctgggaa gattatctgt
     3661 cagcgtcacg tcagtgagat ccagcctgcc cagttcttcc ccgttcaggg tagccttgcc
     3721 agcggtatct gtttcgccct tgccgttata gaggactgcc tgccagatca gggtatcgcc
     3781 agcagtgata tagccgtttt ccaccttctg gagatctgcg ttttgatagt cctttccggc
     3841 ataggaagct actgccagct tggcgaaacc aggggcggga gtggttttgg tgaagctgat
     3901 gtccacctta tcctccggcg agatcacacg atcactgccg tcatttttgc cctccaccac
     3961 tgtcaccttg gaaagggtgt ttgtcagtgt gacttttttc gccgtgctgt ttgccagttc
     4021 ctgtgccttg tcggcggtca gcttggcttg gtaagtcaac tttacgccac cgccccactg
     4081 atttgcaaat ttttctgtgg aaacaaatgt aaaggtcagc gtctgtccaa ctcgagttgc
     4141 ctgaatgcca ctcaaagctg tataatttgc agttccctct acactatttg gcacatattc
     4201 catgccgtcc ggcagcgtat cttctacaac gatcgttttc ccagcatagt cattatttga
     4261 accagaaagt gccaagctgt aactaaatgt tttctgctcc agatctgccg ctgtcatatc
     4321 aacgatattg gtcgttttgt ccgcactatt tgtcagtgac tttttcgcct caaaaacagt
     4381 attgcttact ggattcaaaa tcatgtcatg tgcataccaa gttacagttt tgcttccgtg
     4441 ttcgtttaca gtcagaccgc ctactgcggt atagttatag acaccactct gtgttccgaa
     4501 ctttgtttca aatgcactta atgtctgcat caatgcacta ttattcagat cataaatacc
     4561 accaaacccc tgaattccat ttttttcatg attgtagtca ttattattag tgacatcgtt
     4621 tgcaagtacc acgtattcca ctgtcactga ttcgccagcc gccagcttga ttcccataat
     4681 attaaaggtt tcagccgcat agcctccatg agatctatta tcttgctcta cagtaacagt
     4741 ctgaattctg gagtcagacg cattgccgtt aattgcaacg actttcagaa catcagaagc
     4801 ttcccacttc aacgttgtac cataatcata taagtacagg ttttccatgg tcgtggttcc
     4861 cttgttggta aaagtcgcct gatatgttag cttatcacca tttacaattt ctgttgtatc
     4921 atctgcttgt ataccattta ctgcaatgat tttcttttgc aagctatatt tgcttaaatc
     4981 ttcaccgccg taaatagtat gcgtcgcagt acccttttct tcagcaccct catacacctt
     5041 tgcggtattt gtcagtgttg cagctgttgc agaagcttcc acatttgcat atactgtcaa
     5101 cgtaatcgac tttccttttg gaacagtaac cgttccgccg gcagtcaatg tttttgtatc
     5161 aacagtagta atatcctttg ttgtaccgtc tgccaaagtc agcacaccat atacgccgct
     5221 attctcacgg aatgccatat catttggcag aatatcctct atcttcacat tctcagcatc
     5281 tgcttcaccg ttgtttgtta ctgtaattgt ataaacaaca tcttctgcag tgccaaatgt
     5341 gtaattgccg acatctgccg tcttcgtaat ccccagctgt gctttcggtg tatccggtgt
     5401 aaacttcact ttattggaag acttgtcgtc tacctttgcc acattggaga tctctccggt
     5461 cacgctgctt ttgattgtac cagtcaccgt gatctgcacc gtttttcctg ccggaacgtc
     5521 aatttcattc ggaaaggctg attttgtaac accgtctacc ttataagtta tattctccag
     5581 cttgctcgac aggttatcat ctgcaacagg aacatctttc gcgtcctttg caccggagtt
     5641 ggtaacagaa atggtatagg tcgcaatctg tccggccatg ccggggtagt acttctgtcc
     5701 cagattctta ccattataag ttacatagcc gctttttacc ggcgtttcga tcactgcctt
     5761 gtcttccttg acagagatcg ttgccttggc tgtattgttg ccgtcggtca cttcgttggt
     5821 caggtttgca gtctggtctt tggaaacctt gcagaccacc tttgctgtca ccgttgcacc
     5881 ggcagcgaca ttctggaatg tcacgctgtt tccggaaaca gtaccgtctg cggctgtgat
     5941 gctctccagt gtcatgccgt ccggaacgtt atctttcagg gtaatatccc cggcagcatc
     6001 accggtattc gaagcggaga tctcaaattc tacagtatcc ccgttcacgt agtatgcttt
     6061 tccatttttt acggtttttc caatactgaa cttcggcccc tgcggagcag aaaccttcag
     6121 ttccacgctg tttccgccga tggtcgagta gctggcatcg tcaccgtagg gcttgccgga
     6181 aacagtcagg ctgttggtga ccttgccgtc cgcggtgccg ttcatcttca cggtgctgct
     6241 gttcagaggc agtgccacgt tcagattaac cgccggcatc tcggcattgg tgttcttgct
     6301 gtccacggtg aagctgatct tggcagtgct gccgctgacc gttacactca ggttgccggc
     6361 agtgtagcct tttgccgcaa cggcagactt gacggcgttt tccaccgcgg tctgtgcatc
     6421 accgataaag gtcatttccg acagtgaaat ggtatcctcg aatttcaggg actgcaccca
     6481 ttccacgccg tttttcttgg agacgttgtt cgccgaagca ctcagggtat aatttaccgt
     6541 ggtgccggca gccagctgtg ctgctgtcag ggcgtttctg tcctccgact tggacgcact
     6601 ccactgccgt tctgccctgg cagtaattgt actgctggca ttcttgcctg tgctgtcctg
     6661 tacgatcttt acggtcgaga tcgttccgcc cggggtcttg ccgttctgga acttcgcctg
     6721 cagccggacc tgtttggtct gaccgttgtc aatatcaaag gcgatatacc ggctgccgtc
     6781 gctgttgaca tgcagggtat acccattata aacggctccg tccctgaagc cgtttccggc
     6841 aaagttcggc agcagcagat tcttgtcggt gatctcgatc cgataagaat ccatctctcc
     6901 ttcattgacg ttgtttccgg agatcgtcga cattagatag aacacatctc ccgtattcac
     6961 ttcatcaatc ggattatact tgctgtcccg aaattcgaca ctggcgtttg tcagtaccag
     7021 cggtccgccg gatcgtccgt tattgtccgc cgataccgga aacagcatcg acggcaggca
     7081 ggtcacgatc atcaggactg ccagcaagac actgatgact cttgtacgca ggtgtcttgt
     7141 cttttctgtc tgcataaaaa aactcctctc attgcgggaa cgctgcacgc agtctgtttt
     7201 gcattctttc gtgcaaactt tatgcggcgt ttcctcagat tttttgcacg ggaaaaccgg
     7261 ttccctgtgc agatttgcac cgtggcagca acatcgacaa aaacgatata cataccacat
     7321 tttttacagc aatttccact aactccgcca cttttaactg ttcaatcgtt tccacaatca
     7381 aaagaatatt ttggtgtaaa tcacggaaat tactctgata caactttatt atactgatta
     7441 tctgtgaagt tttatatcac tttttatgta atatccgaaa agcaaaagga aggatcagga
     7501 agtctgttca aactgctttc tgattctcca aattttgcag cggcatctcc ggcgaatggc
     7561 tgtccgaatt tacccgcgtg caacttcttt caatcacata tgacgaatcc cgccattgtt
     7621 ttttcaaata tttcgtgaaa aaagctattg caaccttgcc caaaatccgg tataataaat
     7681 agtaggggat gttccagagg aaacggaaca gtccgcagta tggcaaatcg aagccagcac
     7741 taataaatgg cacgcctatg ccaaatgcat tgaggaggat attatggtag caaaaacaac
     7801 agaaaatccc cagagagaag aattaaaaca aaaattcgta gaccgacttc agctggagtg
     7861 cagcgtttct ccggacgaag cttctgacaa gcagatctat caggtgctgt ctgccctgat
     7921 ggtcgaaaag ctgaagcaca agcggcagca ttttatcaat aaaaccaatt ccgtcggcgg
     7981 aaagaacgtt tactatctgt ccatggaatt cctgatgggt cgttccctga agaccaccct
     8041 gtacaatctg gaactggcag atgatgtccg tgcgatcctg caggattatc acatcaacct
     8101 ggacagcatt ttcgagtgcg aaccggacgc tggtctgggc aacggcggtc tgggtcgtct
     8161 ggcagcatgc tatctggacg caatggcaac acaggaaatc cctgcaaccg gtcactcgat
     8221 ctgctatgag tacggtatct tcaagcagat tctggaagac ggctggcaga ccgaagaacc
     8281 ggacaactgg ctgccgggcg gcagcgtatg gctggacccg aagccggacg aagccattga
     8341 agtccacttc gagggcaacg tacaggaata ctggcaggat tcctatcact atatttccca
     8401 tgtcaactac aacaccgtta tcgcgatccc gtatgacatg tacgtttccg gttacggcag
     8461 tgccggcgtt tctaccctgc gtctgtggaa ggcacaggca cccagctttg acatgaagga
     8521 attcaacgag ggcaactatg aacaggctct gtcccgcaac tccatggcga atgcgatctc
     8581 caaggtactg taccctaacg acaaccatct ggagggcaag ttcctgcgtc tgcgtcagca
     8641 gtacttcctg tgtgccgcag ctatcggcga catcgtcaac caccacatga acgtatacgg
     8701 cacactggaa aacctgcatg aaaaggtagc gatccacatc aacgacacac acccgacact
     8761 ggcaattccg gaactgatgc gtgtgctgct ggacgactgc ggctatacat gggatcgtgc
     8821 atggcacatc gtcaccaaca cctttgccta caccaaccac accgttatgg cagaggctct
     8881 ggaaaagtgg gacgtgaatc tggtcaagca gatcgtgccg cgtatcttcc agatcatcgt
     8941 ggagatcaac aaccgctact gtgccggtct gatggaacgc aacggcggcg acagtgcaaa
     9001 gaccacccgc atgtctatca tcaaggacaa ccagatccac atggctaccc tgtgcgtcat
     9061 ggcttctcac agtgtcaacg gcgtttccaa gctgcactcg gagatcatca aggacagcgt
     9121 attccacgaa gagtatctgg atacgccgaa caagttcaag aatgtcacca acggtatcgc
     9181 ataccggaga tggctgtatc agtccaatcc gggtctgacc cagctgctgc gtgacaagat
     9241 cggcgacggc ttcctgaaag acggcagcga gctgaaaaag ctggacgttt tcgcagacga
     9301 caaggccgtt ctggaacggt tgcggaagat caagaaggaa aacaagcagc gtttcgccaa
     9361 gtatgtcaag aaaaagagcg gtctggttct ggacaccgac agcattttcg atgctcaggt
     9421 aaagcgtctg cacgaataca agcggcagca cctgaatgct ctgcacattc tggcacagta
     9481 caacgccctg ctggaaaatc cggatatgga ctttactccc aagacctaca tcttcgccgc
     9541 aaaggctgct ccgggctact atctggcaaa gcagatcatt aagatgatct gggctctgtc
     9601 cgaggagatc cgcaagaacc cccgcatttc cgacaagctg tctgtatact tcctcgaaaa
     9661 ctactgtgtt accatgtctg aactgctgat gccggcctgc gacatctccg agcagatctc
     9721 tctggcaggc acagaggctt ccggtaccgg caacatgaag ctgatgctga acggtgccat
     9781 tacactgggt acactggacg gtgcaaacat cgaaatcggc gatgcagttg gtccggaaaa
     9841 tatcctcatc ttcggcatga agaccgaaga agtcaacacc ctcaaggccc gcggctatcg
     9901 tccggaggac tattacaaca acaatccggt catcaagagc tgtatcgacc gcatgtatgc
     9961 cggcatcaac ggctgcaagt tcactgaagt ggcagactct ctgcggacac tggatccgta
    10021 catggttctg gcagacttcg acagctattg tcaggtacag gaacacagtt ccaagctgta
    10081 caccgacaag gacacatgga ccaagatgtc cctgcacaac attgccaacg caggcatctt
    10141 ctccgcagac cgtgctgtaa acgagtacgc aaaggatatt tggaatatct gaggactggg
    10201 gaatctttcc cggaactgat ataatctgac gaaaacggag cagcacaaat gaaatgctcc
    10261 ccttttgtta gacaatatgg tataatagaa atataccaat taaa
//
LOCUS       DAWBGR010000084        10248 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1885_length_10248_cov_4.839792, whole genome shotgun sequence.
ACCESSION   DAWBGR010000084 DAWBGR010000000
VERSION     DAWBGR010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10248)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1885_length_10248_cov_4.839792"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5827..5836
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2209 a         3058 c         2566 g         2405 t
ORIGIN      
        1 acctatgacc ctctgcttgt aaggcagatg ctctcccagc tgagctaagc ttccacttgc
       61 ttgtctttcc ggtgtttttt gtgttttcct caccggcgac tattacagta tatcacattg
      121 caaggggttt gtcaagcgtt tttttcaaaa aaatcgaaaa aacgcacact ttgttatgtc
      181 tgtatagttt caacattgtt ccattgtgtg atatgcacat gaaagccgtc taatttctgc
      241 cattttcgcc gctgcgacgg aacagccgca gcacttccag ccgcaacgcc ccatgttcgg
      301 gagccgacag atccgcatct ttttccagaa ttttttttgc cgtttcctgc acttcatgga
      361 gcagctgggt gtcatgggac aggtcggcga tctgtaacgg cggcagaccg tgctgccgct
      421 gcccgaaaaa gtcccccggc ccccgcagct gtaaatccgc ctccgcgatc tgaaagccgt
      481 cgttggtacg gctcatgatc tgcatgcgag tgcggcagtc ctccgtcacg tggtcggtca
      541 tgaggataca gtaactctgt gcactgcccc gtcccacgcg gccccgcagc tggtgcagct
      601 gggacaagcc gaaccgttca gcgttttcga tcagcaaaat ggtagcgttt ggcacatcaa
      661 cgccgacttc aatcacggtg gtgctgacca gcagatccag ttcatggttc cggaacgccg
      721 tcatcacgct gtccttctct gccggcttca ttctgccgtg gagcagtccc acccgatagg
      781 cagagaatgc tcctgcctgt atggactctg catagctgga aactgcctgt aattcgctct
      841 cgtcccgatc gtcgatcatg gggcagacaa tatagccctg atgcccctgt tccagctgct
      901 gccggataaa gctgtacgcc cgctgccgca gctttccggt gactgcaaaa gtctgcaccg
      961 gcagccgtcc gcggggcagt tctttcagga cagaaatgtc cagatcgccg tagatcatca
     1021 gagccagtgt gcggggaatc ggcgtcgccg acatgaccag cttgtgaggg cagccgccct
     1081 tgtccgccag cagcgtccgc tgtgccacgc cgaagcgatg ctgctcgtcc gtaattacca
     1141 gcccaagccg tgcaaaggaa acctctttct gaaaaatcgc atgggtgccc accaccagcg
     1201 tcatgctgcc gtccgccagt gcttcgtaaa tctcccgccg ttccttggca cggacagagc
     1261 cggtgagcag ccccacagac acgcccagcg gctccagaaa ctgctgcatg gtgtgatagt
     1321 gctgcaccgc cagaatttcc gtcggagcca tcagagcaca ctgcatgccg ttccgtgcgg
     1381 caaaatagca cgccgccgcc gcaaccgcag ttttcccgct gcccacatcg ccctgtaaca
     1441 ggcggttcat gggagcatca ccgcacaggt cggctgtaat ttcctccact gcatgcttct
     1501 gtcctgccgt catggtaaac ggcaacgccc cgtaaaacgg ctccagatcg gtatcctgcc
     1561 gcatgggata ggcagaccgc cgccggttgt ccgcacggcg tatcagcatg cccagctgca
     1621 attgcagcag ttcgtcaaaa gcaagccgcc ggcgagccgt ttccatcagt gccggattgg
     1681 caggaaaatg cacctgttcc agtgccgccg tcaggggcag cagtccgtgt tccagacgca
     1741 tctcctccgg catccagtcg aaaggctgct gccgcatcag ttcgatcgcc tgcttgactg
     1801 cccgcaccag aatcggcatg gtaatgccgc tggtcagagg ataggacggg tagatcagtt
     1861 ccttgctgtc gtccgccagc acgtgaggtg cagaaatttc ccgccgcagc agtccgccct
     1921 gcaatttgcc ggacatgcgg taccagcttc ccgtgtgcag agcctgaaag ccgtaggggt
     1981 tgttgtataa gatcaccgtc acgtcattca gcccgtccgt ggcaaccgcc ttgaaaatgg
     2041 tcatgccctt gcggataaac tgcggggcaa gcttgtgcac gatctgcacc cgaatcacac
     2101 agggttctcc cgtaggtgcc gccgcaatgg gaacagggtg gctgtagtcc agataccccc
     2161 gcggcacatg gtacagcaaa tcatagggag tgcggatccc cagcttttcc agagcctccg
     2221 cccgcttggt gccaatgcct ttcaggttgg caaccggctg aaataatgcg tccatgaaac
     2281 gattcctcct ttctgaaaaa tgttgttcga gggaacacag cctcttttgc tctattatag
     2341 cacagtttcc tgcaaaaggc aacaaaaagc ttctccacca acgcgaagaa gcttttttca
     2401 aaaaatctca aagagggata tttgtcttat ttgttgttgt agccgtcaat gacttcatcg
     2461 atcacagagg accactcgtc acgcagtaca gcccaggagt ccttatcgcc gttcagcagt
     2521 gcagaggtca ggttgttcat gacacctctg gtttcatagg tgtattcgcc gtcgccgtac
     2581 attctgctgc ccatgccata gccgaagtcg aatatcggtg tgatgttctt cggatccttg
     2641 taatcctgca acgcatcata ctgttcctcg gtcacatagg acttcagctt gccctgtgcg
     2701 ttgacttccg gaatgatcgc cttttccttg gcagcgtcct tgtattcgtc aatcatagct
     2761 gccagacgct cgcacttgat gtacgctgca actgcatcgc ccttgtcaga attcttgacc
     2821 agcatctttg caccgaagtt gcagttcaga tagtattcat cgctgcccgg ctgcttcggg
     2881 aacggtacga tcatcagatc gttttcttcc acaaagccgt catcttcata gtccggattc
     2941 ggatccgcct tgacattcga tgcacccagt gcccagtctg ccatgccgaa gaacagcgtg
     3001 gagccgtcgt ccggcagata gccgtaccat gtgggatcat acagaccaag gctcatgatt
     3061 tcttccatta cgccctcggc ttcttcgatt gccggattgc tgatgttgtt ggagaactta
     3121 gagccgtcat acttgacaaa tgtttcgccg gtagactgca ccattgcctg accaaaccag
     3181 ccgttgatgc cgtagcgggt ttcgccgtcc ttggcattgg aaacaaacgt tttcatcatg
     3241 tccatgaaag cgtcccagtc ccacttgtcc ttcttgtaca gctcgtaagg atcgtccaga
     3301 ccgttctcct cacacaggga acggttatag gtaatcagca gcggatcaga caggcagtac
     3361 ggtactacat aatgcttgtc cttatacttg aacatttcaa taacatctga catatccgac
     3421 cagatgtcgt cgctcagatc cagatagtcg tccagcggct gatactgttc cttggtcacg
     3481 ccgttgggca cagcgtccca ttcatagggg aacatatcca ccggatcgcc gcccagaata
     3541 cggcttgcca gtgtgtcaaa cttggtgtca gaggtacagg aaacatactc cacctttgca
     3601 ccatatttgt cctcgaacag cgacagggca acgcttctgt cctgattgtt cttcgggttg
     3661 atgtcatagt ctgccagcca gtaaatggtc tgtcccttga tgtccggatc attcttgacc
     3721 atatcgtcta ccagagaagc atcaatggca acgtcgtcct tttccttata ggcagattcc
     3781 gcctcggagg aagaagcact cttggaatcg tccgaaccgc cgccgcaggc acccagtgct
     3841 cccaccattg tcaaagccat cagtgccgcc agcatacgtt ttaaagttct cattcttgta
     3901 aaaaactccc ttccaaatta tatcgcacca aaaacgattc gtgtcacgaa tctagcactt
     3961 gtattatagc ataaaattcg agaaaggtca agcatttcag acggtcaact tgcacaaatt
     4021 gtgggaaata tttttggaaa tcccacagaa cattcccgca caaattgccg gttgcagaat
     4081 tttcaaatat atagtataat agaacctatg cagaaaatac aaggaggcaa catatgaaaa
     4141 cagtacagat cttcacagac ggagcatgca gcggcaaccc cgggccgggc ggctggggag
     4201 cgatcctgcg gtacggcacc gcggaaaagg aactctccgg cggcgaacac gaaaccacca
     4261 acaaccgcat ggaactgacc ggtgtcatcg aagccctgtc cgccctgaaa gaaccgtgca
     4321 gcgttctgct gactacggac agcaaatacg tggtggacag catcaccaag ggctgggttt
     4381 acggctggca gaagcggggc tgggtcaagt cggacaagaa acctgcactg aatgtggact
     4441 tgtggaagca gctgctcccg ctgctggaaa agcatcaggt cacattccac tgggtcaagg
     4501 gacacgccgg tcaccccgaa aacgaacgct gcgaccagct ggcagtgatg cagcgggatc
     4561 ggaatgcgtg atgtcgtatt caccagacaa tgacaggaac attgcccata tccccacaaa
     4621 cggaaaagtc cccactcttt cgagtgagga cttttcctgt attatagggg taaaaatgaa
     4681 aaaatgagat tgtcaaaaat aagattcgcc agaagcagaa ttacttcttc tttctggtga
     4741 tcgtgtatgc accaacaccc agagctgcgg ttgctactgc acccagtgca actgcggcaa
     4801 taccaaagtc accagtctga gaagtggttg cattgttggt accgctcttg gagttgttgg
     4861 aactggaaga accaccggaa ctgctgttgg aagaaccacc attgctgccg ttggaagaac
     4921 cgccgttgct acctgctgcg gtagttgttg ccggagtacc gtcagcgttt gttgctgctg
     4981 tggtagtacc gtctgtgccg ttaccgccgt ttgcagcggt agtggtagta gtagtgttat
     5041 tgccgctgtt gtcagacgga gcagttgtgc cctcgccgtt accgccgttg gagataccgc
     5101 tgacggtgaa gtttacaacg accttatcgc cggcagccaa accgccgtcc ggagaaacat
     5161 gatcgcccgt accaatgtca tttactttcg gctgtgtcca tacattcata atatttacac
     5221 gaatgctggt gccgtcatct gcagtacgca gtgcaccctc agacggacct gcatatgcca
     5281 acggtgtaga accgccgcca acatgctgaa cctcaataga atcgatttcc acctttgcgg
     5341 tgcagccctc cggcagaccg ccggtcgggg tagatgctcc ctccggagca aaagaaaatg
     5401 cattaatatt ggtagaaaga atcatagctt caatggtttc cgaaccgttc accatggtaa
     5461 tatctacaga atagctgccg tcgccggtaa ttgttgcttc cgttgcttct gtaatattgt
     5521 tcttgtctgc aattacagcc ggatcatctg tggaagaaac ccacttctta tccccgcctg
     5581 cctgaaatgc cagaaaagca ttatacggtg ctttatcctg tgccttcaga tcagcagatg
     5641 ctgtcagaga tgcaaaagac aataccgcaa ccgcagatgc cgcaacagct gccatgatct
     5701 tcttcataaa cttcattgta aactcctcct tattcacgca ctgccacgca atgcgtttca
     5761 cattctttca gtattatagc acatttcaaa aaatttttaa agggttaatt tataaaaata
     5821 atatacnnnn nnnnnntttc aaaaaagctg tctatgcaca ttttgactaa atttcaagag
     5881 gaaatccgtt aacttcgaca aaaaccgcac aaaattgcac tgcggcagta ttatttcgca
     5941 ccgtttttcc cagcttttcc taaaacgtcc aagacttcgc gaaacaattc cagcggcggt
     6001 tctcccgcca gttccacgcc gagatagggc ttccccacgc tgccgaactg cacccgtccc
     6061 cgatacttcc gggacagtgc cccgatcatt tccgccgtgg ggtgctccat gtagaacagc
     6121 atgctgccgc cacgctggtt gatctccgtg attcccagct gtgccgctgt gttccgcaga
     6181 cgggacacag taatcagccc ctgcaccgat ttcggcggtt cgccgtaccg gtcgatcagt
     6241 tcgtccagca ggtccatttc gtcctcctga ttccgcacgc cggcgatctt ccggtacata
     6301 tccagccgct ggctcaggct ctccatatag ctttccggaa tatgggcatc gatctgaata
     6361 tccacggtgc actctgccgt ttcctgtatc ggctcgccct tggcttccgc cacagcttcg
     6421 cccagcagcc gcaggtacat gtcatagccc acggattcca tgtgtccgtg ctgtctgccg
     6481 cccagcaggc tgcctgctcc gcgaatttcc aagtcccgca gggcaatgtg aaaaccgctg
     6541 ccgaactggg taaattcccg cattgccgcc agccgccgcg tggattcctc ccgcagcacc
     6601 ttgtcccgct gaaacgtgaa ataggcatag gcacggcggt tggaacgtcc gacacgtccc
     6661 cgcagctgat acagctggga cagtccgaag cagtcggcat cttcaataat gagggtgttc
     6721 acgttcggca cgtccacgcc ggtttcgata atggtcgtgc agaccagaat atccgcctca
     6781 tgttccacca gcttccgcca gatctccgac atttccgcct cgcccattcg cccgtgtgcc
     6841 accagaatcc gtgcgtccgg cagataccgc tgcagcttcg ccgcggtgaa ttcaatggtg
     6901 tcaatgcggt tgtggatata gtacacctgt ccgccccgtt tcagttcccg ctgaatcgcc
     6961 tgcaccagta ccggctcgct gtactccatg acataagtct gcaccggata gcgatcctgc
     7021 ggcggctggg agatgacgga catatcccgt atgccgctca ttgccatatt caatgtgcgg
     7081 ggaataggcg ttgccgacaa ggtcagcata tccacgccgg caaacatgtg cttgaacttt
     7141 tccttgtgtg ccacgccgaa ccgctgttcc tcgtccacga tcgccagccc cagcgaacgg
     7201 aattccacgt ctttctgcac aatacggtgg gtgccgataa tgacatccgc cttgccgctt
     7261 ctgatctctt tcagaatctc tttctgccgc ttctggggaa cgaagcggga cagcatcacc
     7321 acgttcaccg gaaaatgttc aaaccggcgg actgccgtct gataatgctg aatggcgagc
     7381 acagtggtcg gcaccagaat ggcacactgt ttcccactca tgacgcattt cattgccgca
     7441 cggagagcca cctctgtttt cccgaagccc acgtcgccgc agagcagccg ttccataggg
     7501 cgttcctgct ccatgtcgtg cttgatctcg tcgatgcatg ccagctgatc ttccgtttcc
     7561 acataaggga accgagcctc aaagtcatgc tgttccggac tgtccggctc aaaggcaaag
     7621 cccttggtct tttcccgctt ggcatacagt gccagcagtt cgtccgccat atctttcacc
     7681 gcacggcgga cgttgctgcg tgtcttttgc cactccggcg aggacagccg gctgagtttt
     7741 acgttggaat cttcccgcgg accgatgtat ttcgtcacca tgtccagctg tgtgaccggc
     7801 acatacaagt tttccttgcc ggcatactga atggtgatgt agtccttggt cacgccctcc
     7861 agttccagct tgcggattcc cagaaaccgg ccgataccgt gcagggcatg caccaccaga
     7921 tcaccgggga caatgtccgc cagtgtccgg atttcctcac cgcggcggtg ctgtttccgc
     7981 cgccgtgtgc cggcgtggag tgctctgccc tgtgtgatga gagccgtttt ggtttccgga
     8041 taggcgaagc cgccggacag gctgccggtg cggagataga ccacgcccgc acgccacacg
     8101 ctgtccggtt ccgccagctc gcagcggatc ccgctttcct gcaagtcctg ctgcaagatc
     8161 ggcagcgttt tttcactgcc ggcacagagc atggtgcagt agccctgact gctgtactcc
     8221 tgtaagtcct cagtcagctg ccgcagttcc ccgccccacg gggcgttctg gactgcctgc
     8281 acactgagca gcttctgaaa ttccacatgt tcgccgccct gtaagaaatt gctgcaatac
     8341 acctgaaagc gtttgtccag cttcgccgcc gtttccgcag ggtcctgata atagccctcc
     8401 agaccgcggc aaaggctacc ctcctccagc agcagcttgc agtcctcctg atactgtgcc
     8461 atcagcccac gctggctctc cacaatggca ccgtattcgc tccagcagac gatctccatg
     8521 ccgtaatcat acagcatcgg cggctgctcc cacagcagcg gggcgtactt atccagatgc
     8581 accggaatca tgccggcttc cagatgatcg gcatctgcat aaagcttttc ccgcaccaga
     8641 tcggcacggc gaatgcggag cgactttccc agcatacgaa ttttcttcgc cagctccgca
     8701 gggtcgcaga gggtttcccg tgccggcggc accagcacct cgtccagcgg gtctgtccgc
     8761 cgctgggttt ccggatcgaa atagtgcatg gtgtcaatcg tatcgtccca gaattccagc
     8821 cgcaccggag ccgaaagctg caccggaaag atgtccacaa tatcgccgcg gacggagaat
     8881 tgtccctgac cggaaacgct gtccccgcgg acatagccgc agtccgtcag ctttctggtc
     8941 agttccgaca ggtctgccgt gccgtcccgc tgtaacaata tggtatgttc ccgcagctgt
     9001 tccggcgaaa gggtgggctg gagcagagcc tcgatgctgg cggcaaccac cttgcactgc
     9061 ccgttctgca acgcagccag tgctccgatc ctggcgtgtt cataggcagt ggtgatgccc
     9121 tctaccggtg tcagaatcac ttctttcgcc ggaaataccc atgccgcagt ttcttccagc
     9181 atggtgttga catcatcaca gagccggcgt gcctctgctt cgtcggcagt gatgaccaga
     9241 accggtgccg aggtctggct caatgccgcc agcaggtgtg ccttgtgaat ctgggaaacg
     9301 ccggtaaagg acaccggtgt cacaccctcc tgccttgccc gcatcagttc ctgatatgcc
     9361 gacagctgtt ccattgttgc ggtaaaaaac tgcatggctg attcccctgc ctttcgtttc
     9421 taagaaaaca cggattagtg actgtacaga ttcattgcgg tgttcatgtc gccgcccacg
     9481 atcttcacgg cagcatctgc ggcgtgttcc agtgccggct gcagggcagt catatcctcc
     9541 tcgctgaact tggacagcac ccagtccgcc agatcatact ggggatttgg cttcgccccc
     9601 acgcctacgc ggatacgggg aaattcctcc gtgcccagct gtgcgataat gctcttgatg
     9661 ccgttgtgtc cgccggcact gccctttcgc cgcagccgca gctttcccgg cggcagggag
     9721 atgtcatcat aaatgaccag cacctgctcc ggcgggatct tgtagaagtt cgccgccgcc
     9781 gcaatggcat cgccgctgag gttcatgtat gtcgtcggct gcatcagcag gcagcgtact
     9841 tcccccagca tcgcctcgca gcagttggac tgaaacttca tctggttcag ttttacgccg
     9901 ccgcactgtt tcgccagttc ctccaccgcc agaaagccgg cattgtggcg ggtgtgttcg
     9961 tattccagcc cggggtttcc cagaccggca acaatccagc tcagcttgcc gtgcactctg
    10021 ccggcggaac ctgactcgat ctgctgaaat aaatcaaaaa tgcccatttt tcctataacc
    10081 tgcttcatgc ggcaccagag ccttccgatg ccgcatgaat cctttcgcta atactgcttt
    10141 ccgttttttg tcagcgagca cagcatgctg taccctgtcc tctaggcaat accacacaaa
    10201 gattgtgcga cagatgcgtc gtcaaatttt tcgtcagatg aaacaaaa
//
LOCUS       DAWBGR010000085        10055 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1921_length_10055_cov_3.566400, whole genome shotgun sequence.
ACCESSION   DAWBGR010000085 DAWBGR010000000
VERSION     DAWBGR010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10055)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10055
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1921_length_10055_cov_3.566400"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4285..4294
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2513 a         2655 c         2116 g         2761 t
ORIGIN      
        1 catcgacacg gccaaggttt tcaccattta caatattaat gacatctttg caacgcagtt
       61 cttccagcgt acacaccaca gtgcatcacc tctgcaacag gatatgcaag attctgcgaa
      121 ttctttcaca aaaacaattt gaaaaggacg gtacaaatca aatccagtcc cattataact
      181 gctgctgtga tgctcactac gtgcaatacc ttttccgaca tcagtccggt aattttggca
      241 aacagcgggc gaagcagata caaaaacaga acgccgccga tgccaaaacg aacagacggg
      301 gaaagagcaa ctcttgcctg aaaattgatt gcatagtctg cataggtttg ccatggccag
      361 cttccagttg tccattccaa cagatagctg accgccaatt cgatcaatgt ggcaactgcc
      421 ccacaaccca gaaaaatcag aatcggtctt gcccaacgta cacgccatga tagttgcatt
      481 tttgcaaatt ttccaaagca gagcaaaaat gccagcatgc cgactccata taccggacaa
      541 tacgggccaa acagtacgcc tcggttttga aacccccaac gatagacaac aacttctaaa
      601 aacacttcat agcaccagcc aataattgct gccatgagaa aatagaggaa caatttttca
      661 attgtttgca gtttattttg catttctgtc ctccctccaa tcatcatctg tctaatacaa
      721 aagcaggtgt tctcacaaaa atagaaacgc ctgcttttgt atcctatgtg tacttatgaa
      781 aaaattactt cttttcggta actgcctttt tggcaactgc ggcaatgttt tctgcacaca
      841 gaccaaattc cttcagcagc tctacagccg gaccagaatg accgaactca tcgttcacgc
      901 caatgcgaac aaccggaacc ggacagcatg cagtaactgc ttctgcaaca gcaccgccca
      961 gaccaccgat aatactgtgt tcctctacag ttaccagcag accagtttct gaagcagcct
     1021 tacagatcag ttcagtgtcc agcggcttaa tggtgtgaat attcagtaca cgagcgtgga
     1081 ttccttcggc ttccagcagt tctgctgcct tcatggattc ccataccatc agaccagtag
     1141 caaccaaagt tacatctgtg ccttcccgca gtgtaatgcc tttaccaatt ttgaatttat
     1201 aagttgcagc atcgttgaag atcggagttg ccagtctgcc aaatcgcaag taaaccggtc
     1261 cctcatactg aatggcagct tctacagcag cctttgcttc tacatcgtct gccggattca
     1321 aaattgtcat acccggaatg gttcgcatca gtgcaatatc ttcgttgcac tgatgtgtgg
     1381 caccgtcttc accaacggaa ataccagcat gagttgcacc gatttttaca ttcaggtgcg
     1441 gataaccgat agagttccgc acgatttcga atgctctgcc ggcagcaaac atggcaaatg
     1501 tgctggcaaa cgggatcttt cctgccgtgg aaagtccggc agcaacgccc atcatgtttg
     1561 cttctgcaat accgcagtcg aagaaacgct ccggataagc tttcttaaaa atgcctgttt
     1621 ttgtggcagc agccagatcg gcatccagta cgaccagttc cggatatttg tcagcgagat
     1681 cacgaagtgc ctcaccatag ctttctcttg ttgctttttt ctgcaattct gccatcgttt
     1741 aaccctccag ttccttcagc tgggctgtca gttcagccat tgcctgttca tactctgcct
     1801 gattcggtgc tttgccgtgc cagccgacct gattttccat aaaggacaca cccttgccct
     1861 tgacactttt ctgaatgatc gcaaccggct tgcctacgat tgtttcagct tcctgcattg
     1921 cagcatcaat tgcatcgaaa tcgtgtgcgt ccatacgaat gacatgccag ccgaacgctt
     1981 caaacttttc cggaatcggc tccggcgagc aaacttcgga aagcttgccg tcgatctgca
     2041 aaccgttgtt gtctacaatt gctaccagat tgtccagatg atactggttt gcgaacattg
     2101 cagcttccca aacctgtcct tcttcgattt cgccatcacc cagaatggtg taaaccttat
     2161 aagcagcatt gtccattttt cctgccagtg ccatgccgca ggctgcggaa atgccctgtc
     2221 ccagagaacc gctggacata tctacgcccg gaatatgaat acacgggtgc ccctgcaaaa
     2281 gtgcgccgat atgacgcaga gattgcagtt cttctttcgg gaaaaatcca cgttctgcca
     2341 gtgtggaata cagagccggt gcacagtgtc ctttggaaag caccagacga tcacggtctg
     2401 ccatttccgg attcttcggg tcaacgtgca tctttgcaaa atacagataa gtcagtgtat
     2461 cacaaatcga gagagagccg cccgggtgac cggactttgc gtgataggtg ccttcaataa
     2521 cacccatacg aatacggcag gcagttgcct gcaatttttt cttggtcatt tcatccatgt
     2581 tatgaacctc caatgatttc gaaagacgat aaatcaattc tgcaatcgcg ttttcttcac
     2641 aggaatgcat caactgtata ttggcaattt ctttaatgca gggctgtgca ttagacggac
     2701 aggcactggt gtctgctacg cgaagcatat ccagatcgtt gtcaaaatct cctgccgcca
     2761 caatatgcca gctttccgca ccgcatatgg tgcgataccg ccgaagggca ctccctttag
     2821 taacgccttt cggcagcatt tcataaaaac gtgcttcaga ctgtacaaaa tctgcacacg
     2881 tccagttttg ttcttgaaaa taggcaatca aatcaggcat tcgttcgggt gcaattgcaa
     2941 acagcacttt cagccaacca tgcggaattt cctccggcat tgccagaatg ggttccacct
     3001 gacagatctc cagatgctcc ttttcataag gtgtcattct ggaaacatag gtgccgtctg
     3061 tgcggagaat ctccgccgaa acgtccggaa atgcttctaa cacttgtcgg gtaatcgata
     3121 cggcttctgg cggcaattct tctgtgtaaa gccacttttc tgctaccgga tcataaatag
     3181 ctgctccgtt gaacaatatc acaggaatat tgggctgcag ctttttcaaa tatcgctgag
     3241 ccgcctgcaa cgttcttcca gttgcaattg cgaatttgcc accttttgca cggaactggg
     3301 aaattgctgc tgcatctgcc acagaaatct ctttgctggc tggcagcaat gtaccgtcca
     3361 ggtccgtaat aagcatggta ttttccaaag actgcatcaa aatcaccctt tcaggatttc
     3421 agttttcgaa gtaccttttc aaaatattcc ggcaaaggac tttcaaactc cagatactgc
     3481 cctgtagacg gatgaataaa tccgattttg gcagcatgaa gacactgccc ttcgattccg
     3541 ggaaatgctt ttccatagac atcatcaccc agaatcgggt gacccaaata cgctaaatgt
     3601 actcgaatct ggtgtgtgcg tccggtttcc aatcgcagcc gtaaatgcgt ggcacgggga
     3661 tattctgcaa tcacagtata gtgcgttgta gcaggtttgg aatttttttc tgtgacacac
     3721 atttttttgc ggtctaccgg atgtcgtccg atcggagcag aaatcgtccc ttctgcttcg
     3781 cgaaaatgtc ccattgccac cgcctgatat tctcttgtaa atgaatgggc ggcaatttgt
     3841 gccgataacg actgatgtgc catatcattt tttgccacca ttagcaagcc gcttgtaaaa
     3901 cgatcgatac ggtgcacgat tccggggcga attatgccat tgatgctgga aagccgatct
     3961 ccgcaatgat acaatagtgc atgtaccagc gttcctgtcg cgtgtccggg agccggatgt
     4021 acaaccatgc ccttgggttt gttcaccaca agtaagtcat catcttcata gacaatatcc
     4081 agcgggatat tttccggctg caattccagc ggctctggtt ccggcagctg caccaaaact
     4141 tgatcccctg ctttttgccg ataatttttc ggaacttttt tgccatttac tgtcacatca
     4201 ccgtgttcca tacgaacttg cagggcggaa cgcgtcatcg caagactctc ctgtccggaa
     4261 agccatttgt ccagacgttc tccgnnnnnn nnnntccaca aacaaaaagt aaattgcaaa
     4321 caaaacaacg ccaattacca cgcaacaatc tgcaaaatta aacacggcaa agtgaaacag
     4381 cttcacttcc agatagtcta ccaccagacc accgcgaaac agtcggtcaa ttaaattgcc
     4441 cagaccgccg cttagaatca aggtcaagct gactgtcagc agcttggatt tttttccatg
     4501 acgtaccagc cagtaagcac aaaacgccat cagaatcacc gttgttccca ctagcagcca
     4561 acgcatgccg gcaaaactac tgaaaacagc tccgttattt tccagataag tcaaatccag
     4621 aattttatga tcgccaatgt gcagaaacgg tacacttccg actggtttca aatcctgaac
     4681 gacccagtat ttgatcagtt gatctgctgc ggtcagcaag ataattccga ttaaggctgc
     4741 aatcagcaca aatacatcct ttcccgatta cagctgatct acaacagaag cacaacgtgc
     4801 acagagtgtt gggtgtgcag cattcttgcc aacactgtcc agatacatcc agcagcgttc
     4861 gcacttttca ccagcggcac gttctactgt ataggtagtt tctccggatt ctgcctttac
     4921 aacagaaaca acagatacga tcagcacctt tgccagaatg tcagcagctt ctgacaatac
     4981 tgtatactga gcatcatctg caacagcgat tgtcaccttg gcttccagtg atttaccgat
     5041 cagcttttca tttctctttt cttccagaac cttattagct tccatacgag tctgatagat
     5101 cagatcccac tttgccataa agtctgcatc ttctgtcaat ccgcttgcct tgggcatttc
     5161 gttcaggaat acactcttgg catcatcgct tgcagcatgc ggcagataag accagatttc
     5221 ttctgcggta aaggagagga tcggtgcgat cagacgagtc agtgcactca aaatccgata
     5281 aatcgtggtc tgtgctgcac gacgagccac ggaattttcc ggttcgcagt acagacgatc
     5341 cttgatgatg tccagataga aattggacaa atcagtggtg cagtagttat ggattgcgtg
     5401 gaatacgatg tggaagtcaa atgcttcata gccctcatgt gccttttgga ttacgttatc
     5461 cagacgcatc agtgcccagc gatccagttc cagcaactga tcgtcggata cgctgtcgcg
     5521 atccggatgg aatccgtcac cattgcccaa gttacccaga atgtaacggg cagtgttccg
     5581 gatcttctta taggcatcgg acaactgtcc caaaataccc tctgaaatac gaatgtcaga
     5641 gtgataatcg gaagaagcta cccacagacg aagaatgtct gcaccgtatt tcttgacaat
     5701 ttcttccggc ataatgccgt tgcccaaaga tttcgacatc tttctgcctt cgccgtcaac
     5761 gacccagccg tgtgtgcaga cagccttata cggtgcactg cccttataaa cgacagaagt
     5821 cagcagagaa gactggaacc aaccacgata ctggtctgca ccttccagat acagatcagc
     5881 cggccactgc aagcagtcat actgttccat gacagcactg tgagaaacac cactgtcgaa
     5941 ccatacatcc atgatgtcat attccttggt gaattcctta cagccgcatt cgcactgtac
     6001 ttccggagaa agaaactctg aaagatcctt tgtccaccat gcatctgctc cctctttgcg
     6061 gaacaattct gcaatgtcgc gaatagtggt atcattgaca atcggctttt tgcagtcctt
     6121 gcaatagatg atcggaatcg gcacgcccca agtacgctga cgagaaatac accagtcgct
     6181 gcggtcacga accatgcctt tgatgcgttc ttctccccat gcaggaatcc acttgacatt
     6241 ttcaattgct ttgattgctt cgtccttaaa gccgttaaca gagcagaacc actgttctgt
     6301 tgcacggtaa atgatcggtt tgtggcatct ccagcagtgc ggatactggt ggacaatttt
     6361 ttctgttgcc agcattgcac caacttctgt cagcttcgca gcaattgcac ggtttgccgc
     6421 atcggtgtcc atgccttcaa actcgccggc ttccttggtc tggcgaccct tgtcgtctac
     6481 gcagaccaga atgccaatgt cgtcatagtt cttgcagact tcaaagtctt ctacaccata
     6541 gcccggtgca gtatgaacgc agccagtacc gctttccaat gttacatgat caccgacaat
     6601 taccggagaa agacggtcat acaacgggtg cttagttttg acatgttcca gttctgcacc
     6661 agtgaagctg ccgaccattt cgtattcggt aataccagct gctttcattg ttgcagaaac
     6721 cagttccgca gccatgcagt agtattcctc gccaaccttg accagtgtgt agtcataaga
     6781 cggtcccaca caaattgcaa ggttgcccgg aatggtccat gttgtggttg tccagataac
     6841 aaagtaaacc tgttccttgg caattcccag ctttgtaaac agacccttgt cgtcagtcac
     6901 ctgaaatttc acatagatag aataacaagg atcgttttcg tattcgattt cagcttctgc
     6961 cagtgcagtg ttgcagtcag ggcaccagta aaccggcttc aatcccttgt acatgtaacc
     7021 gcgttttgcc atttcaccga atacttcgac ctgacgtgct tcatattccg gcttcagtgt
     7081 caggtacgga tcacagtaat gtcccagact gcccagacgc ttgaactgtg ccatctgatt
     7141 cttgacatgc atcatggcga agtctttgca gtgctttcgc agttctaccg gcggaatggc
     7201 accattttct acgccgatag acttcattgc tttcagttcg atcggcaaac catgtgtatc
     7261 ccagcccggc acatacggtg cacagaagcc actcatattt ttatacttga caatgaaatc
     7321 tttcagtgtc ttattcaaag cagtacccag atgaatttca ccattggcat acggagggcc
     7381 gtcgtgaaga atgtacttcg gcttcccttc atttttggcg atcatctgtt catacagatc
     7441 ctcgtcttcc cacttttgta aagtgtcagg ttcccgcttc ggcaggtttc cacgcatcgg
     7501 aaaatctgtt ttaggcaaat ttaaggtctt attgtaatcc tgtgacatag cttgatccgg
     7561 ctttttctcc ttttcagcgt atcgctgtgg caaaagccgc tcctttccct gaaattagtc
     7621 tttatcctgc aagtcgccga atctggagtc atatgctccc tgaattgctt ttgcagaaaa
     7681 cggttccgca tcaaacggat cgcttgcagc tggtgcagcg gctactgctg gcttcggctg
     7741 tgccggttct gtcgcatttt cttctgtcat accaaaggta gtgtcatctt cttccggcat
     7801 agcagagagc atgttcagat gctccttgta catgtcaaag agagcttttt tgaaatcagc
     7861 gacctgcttt tttgcagctg ccagtttctt gtgctcctcc tcgatctctg cctcattctt
     7921 ttcaatgagg gcatcgtgct gtacagttac ctcgtccatc attgcatctg ctttttcttt
     7981 tgcctgtgca atgatctctt ctgccttggc gttggcttcg gaaatgatgc ggtgaccctc
     8041 cttttgtgca ccgaggagtg cgtccttcaa tgcatcttca tcacgcatgt attcccgtac
     8101 cttgtctgcc agaacctgaa ttttgttgtt gctgtcatcc agttctcgct gggcgattgc
     8161 agcttcttct tccagcttat tcaggaattc atcaatgtct tcctgtttat agccgaaagc
     8221 agctttttca aaccgtaatt catggatttc gcttgcacta atcattatgg tatctcctct
     8281 cataagagcc tatgcagcct atcctacatt agccctgata tttttgaatt gtgatatggt
     8341 atcttccttt tcggctgatg ccatcgaccg cggcaatacg aaattttccg tagccacgaa
     8401 ttgaaaacac atcacccacc tgcattacag cagaaggcgt atcgatcgct gcatgacgat
     8461 aggtcagccg gccttgccga atcaactgca ctgccttttc ccgactcaga cgcgttgcca
     8521 gtgcggcaac tgcgtctgcc cgcagagatg caatcgtgcc ttgtatcagc aggaaatccg
     8581 tttgcaccga caggcagacc ggatcatctc ccgtcacgct gacaccaact cgtccaatct
     8641 gatgaacttc ctgtaaaagc acaggagcca catgggcaca gacgaacatt tgtgccatct
     8701 tcgtatcgat caaaatgtcg ccgatcgtat ctcgttccag attgcatgcc atacatgctc
     8761 cgagaaaatc ccgatgtgtt ggcggcttag gaacacgata agaaaacgtc agacactgga
     8821 ttggaaactg ttccggtgta accggaatga aatccggagc aaaacaaagt attacacgag
     8881 tggtgttttc tgttccgccc caagctgtac agactgctcc tgctgtattt gcaatcgctt
     8941 ctgccattgc ggtatcttct gccggtaaaa aatccgtaaa gtacggacgg ttgtcccgca
     9001 cgcatcggtt cgccagatcg ttccaatgtg cttttagttg ctgcaccgca tctgccattt
     9061 tcatgtttcg gcttgccatt tttgtttgtt aacctaccag accgttgctc tgcagttcgc
     9121 caacgaggct gtcgccgtca aaaccgacat tctgcggaat aatcaggaaa gtctggttgg
     9181 ctaccggctt gatccgacca tgggttgcat atgccgcacc aactacaaag tcaatgatcc
     9241 gacgcttttc atcatcgtca atcttttcca gattcagaac aacagtctta ttctgcatca
     9301 aataatctgc aactttcttg gagtctgaag tataggaagt tgcctgtacc agcactacct
     9361 gaaactgtgc agttgccttg atcgttgcat agcgttcatt gccgcccttt cgttcttcat
     9421 aagaagacgc acctctgctg taactagaat tgctgtaacc agaggtctgt gcagcgtggc
     9481 tctgcggcgg agcctgttct ggttccggcg gagtcatgct cggggtttcc ggctcattat
     9541 catcagccgg cataaagaag tctttcagat tgtctataaa attcatgata ctctccattc
     9601 tccgcatcag cggatttcat tttatttctg ataatttctt gcaccgaaca aggctgtccc
     9661 gatccggaca agtgtcgaac catagcgaat tgcggtggca aaatcccccg acattcccat
     9721 cgacaaaatc tccatatcta tattatccat ttttttggag gaaatgtcaa gataaagttg
     9781 ttgcattttg taaagaaatt tctcatcacc ctctactggc gggattgtca tcagcccgcg
     9841 tacatgtacg tgggacagtt gtcctgcctg ccacaacaat tcttctaatt gatccggtgc
     9901 aatgccgctt ttggattcct ccccgccgac attaacttca atcaagatgt cctgcacttt
     9961 attgacacgt gctgcatgcc ggtcaatttc agatgccagc ttgatgctgt ctaccgaatg
    10021 aatcagttcc atgtcctcaa tgatatactt tacct
//
LOCUS       DAWBGR010000086        10042 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1923_length_10042_cov_3.352458, whole genome shotgun sequence.
ACCESSION   DAWBGR010000086 DAWBGR010000000
VERSION     DAWBGR010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10042)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10042
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1923_length_10042_cov_3.352458"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2577 a         2520 c         2339 g         2606 t
ORIGIN      
        1 gctctgcact tgccgccgga acagtgcgtg gcaattgagg attcgcccaa cggcgttacc
       61 gcaggactgt ctgcacactg taatgtgatt ctggttcccg acctgacacc gccggaagca
      121 ggggacaggg aacgcttgtt tggcatcgcc gaaaatttaa aagaagtcat tccgctgctt
      181 caggcggctg gcataggaga aaaagaacat gcgaaaaaat cctgcatcgg aagtctatga
      241 cattccacag tacacctatt ttgaattcgg aaacaccttt accggcagct acggcaagct
      301 tagctataag atcataccgg gggagaactt caccgtgcag atctggcaca gccgtctttg
      361 ttcggaactg gcagacatcg aggaggaaca gacgtatccc atgacagaag acgggttcca
      421 cgaaatgctg cgttggctgg aaacgaaagc acctaccgga aaataacaac aaaaatcgtc
      481 ctgaagaaag tctgtttctt caggacaatt ttacttttat gatgacagtg aatttttttg
      541 tgaatcattt atcttttaca tagtatacta attttatttg attgagaaca tataaaatta
      601 gggcaatact gaataaagct cgtgtaacca gattgcgtgc aaagccgtca gacgggcttt
      661 atgcagcgtt ttcttagcgt tcacacaaca cactaaaaca aagacaactg ccgatgacat
      721 tccggaaatt cccgcaagta ggcgaaaatt tgatttggtt catacatgac atcatacttg
      781 gcacaagact cgcggaaaat tgtcatgagc cgtgcctgat tgggactgga gcactcataa
      841 gcatttccaa accgacgtat gtactgctct ttcatgccgg ggaaatgctt gtccaactgt
      901 gcataaaaga attcccgatc gccttcccgc agagtaacgc ccataccgaa acagcaaatg
      961 ccttttacac cagcatcaaa acagtattcc agaatgccgc ggagattttg ctctgtatca
     1021 tttaaaaacg gcaaaatcgg cgtgagccat accaccgttg gaattccatt tgcctgcata
     1081 atcttcagca cttcataccg ccgtctagtg gtgcagacat ggggttccag aatgcgacag
     1141 agcgtttcgt tccacgtggt caacgtcatc tgcaccacac actttgcatt ctgctgaatc
     1201 ttttgcagca gatccagatc tcttaaaacg cgatccgact tcgtcaatac cgttgcacca
     1261 aatccatatt ttgcaatcaa ctccaggcat tgccgtgtca gccccagctg ttcttcgcag
     1321 tgtaaatacg gatcgctcat ggcaccggtt ccgatcatgc agagccgccg ctttctccgc
     1381 aaagcttcct ccagcaactg tggagcgttt tctttcactg ccacatcttc aaaatcgtgt
     1441 cccatttgat agcatgccga acgactgtcg caatagatgc agccgtgtgt gcaaccacga
     1501 tacagattca taccatttgt ggcagataag atgttttttg cttgtatccg atgcatgttt
     1561 acttctttcg cagttccgca attgccgcat aatgccgtac caaaaatgtc tccggtgcaa
     1621 tacgctccaa caactgcaaa tgttcttttt cccatgccgc aatttcttcc ggcgtcagcg
     1681 aagctccgat accgcggcaa gctttcatac gaccgttcca gctggtacgg gtaaaggaca
     1741 cgtccaattc gtattcctcg tgataggtca gatcaaaata ttccaacagt tccggtgcaa
     1801 cggaaatcgg gtgcttggtt tctcctgctc cactccattt tggattatac tgcaacacca
     1861 gtttttcgct ggcttcagca atcttgtccg aatatggcag ccattccatg cagagcagca
     1921 acagttcccc atgcggcttc agaagccttg caaataccgg agccgccttt tgattgtcaa
     1981 agtaccagta gcactgacag gcagtaatgc agtcaaactg ctttccggca aagtccagtt
     2041 cctccgccgg cgaaacaaga taggagattt ccatacccgc cgaaagccgt tttgcctctg
     2101 cgatctgatt tggagcaata tcgatgcctg tccaatgtgc cccgaaccgg tacatatttc
     2161 ggggcagaac gcccgttcct gttccgatgt ccaacacttc ctgtccggca gtgccgcaat
     2221 ttctctccac tattttctga taaaataacg ggggatagat gtcccgaaac tttccgtaat
     2281 cttgagaagt ctttccccag tcgaacgcgt tgccgctgca taaaacgcac tcctttccgc
     2341 acgctgataa aaacaaaacc cacctaaagt aaaaccttag gtgggttggt acgcctgatg
     2401 cgattcgaac gcacgacctt cagagtcgga gtctgacact ctatccagct gagctacagg
     2461 cgcatatcgg aaaaacatca tcagaatcat atctgtcttg tctgacgact tatttagtat
     2521 agcacattct caggataaat gcaagaggtt ctgcggaaat atccgagatg ttcacagaaa
     2581 attttcagag aatctggaaa cgcagtgatc ccttaccagt ctgtcagcag ctgccaaagc
     2641 agtaccgcac catttccggc agtgtccggc agtgctgcca gccagagcca ctgagaatcc
     2701 aaccgcggca gttcccaacg atactcctgt gccgcaagac aaagtgatgc cgtctggttc
     2761 ggctgtatgg tcaattgtgc catagcggcg ggagtatggg tcatacggtt ttgtgaacgt
     2821 gtctgcacag tcagatatgc ccagaaaaac ccgcagagca atccgtaaag acacagccct
     2881 tgcaaaagcc gcctccaacg gcggcgatga gtttgtttca tgttgctttt attccttttt
     2941 caatttcaaa agtgtttgag ccagagcctt tcccaccagt tcaccggcat actgtgcctg
     3001 ctccagtgtg tttccgtgag tgccgatttc cagcagcaga ctgcccgtag tcagatcctg
     3061 attatagtgc cggtaatcaa acagaattgg tctggtcagt cctgcataca tgccttccat
     3121 attttcttgt aaccgacagg caaagtgata attctgcatg tagtttggca tgttcatcgt
     3181 accgtcatca caccccgaaa taatcatcac ttgtgcagat tctttgccgt tgatttccac
     3241 aaccggctgt atcagcgtgt tatccttttg aatcgcgtcc cgatgaatgt ccagcaccac
     3301 ccgaatagac ggatactgct gtaaaatctg tctgactgtt tcagcactgc ggtcatagga
     3361 accgttgtag gaagggtagt cgtgaatggt cgtattgtgg atcacgccaa tgccggcagc
     3421 ctccagctgt gcagtaatag catctccaac cgacaccata ttatacgccg gatctgttgt
     3481 ccgatagttg aacgccggat caaaattttg ccgcacatac ggttcaaaag attctgtcgt
     3541 gtgcgtatgc ataatcaaca cctgcggttc gccgctggtt tcgatcgtga atgccggtgc
     3601 tgccgtactc tctgcaatca gcgtattgtt gggaatgctg gttttgttct gtacctgccc
     3661 cgcggaaaac aaactgaaaa accgtgtgcc ggaatacgtg ccataactgg tacgctgcac
     3721 actggtgtct gaactaagat tccagctttc cggatacggc ttttctccgg catctgcgga
     3781 ggctttttgt tccggcagtt ccgccgaaat ttctgctgct actggtgcgg tttcttcctc
     3841 ccagccatag ccgtcggaaa acacattcca tgaaacagac tgtgcagcac cctctttttc
     3901 ggcataggca tgtgcggcag acttattttg tgcatcatta tctttttgca gaaccgcagc
     3961 agtcggcaga atcaccattg ccgccagaaa gcaagtcagc gtccaccgcc ttttatgaaa
     4021 ccgtctgtgc ataatgaaag acctccctat ttttcgtccg gcagaatctg cctcataatt
     4081 tccgcagtac tacaatttcc aaatcggttg taacggttga tgccatatag acacgctgga
     4141 tttctggtaa tgaagttcgg cttttccaga caagtcagcg taatctgaaa gcccagttct
     4201 tccagcacca gtttactctc cggacagaca aatccatagg gataggcaaa cactgtaggc
     4261 tgtatgtgta gattttcctg aaattccgtt tgcagcatgg aaagatctgt atacagtgct
     4321 tcgtgatagg cttcctccgt ttcctgttcc attttggcac agcccctgcg ggtgccattt
     4381 tcatgcatgt cataggtgtg acttccgaga gtgatccgtc cggaatcttc taactgctgc
     4441 atatcttccc acgtcagata ggaatatata tcgttgtgag gagcgtccgg agcatagaca
     4501 tctgcaaaac tgcccacaac ggaaatcacc gcacacatat catacttttc caacaacggc
     4561 agtgccagac taaggttgtt atataagccg tcgtcaaatg tcagcagcac cggcttctcc
     4621 ggcaagtcac cagtaccctc tgtataggcg atcaattctt ccggagatac tgctgtgtag
     4681 tgatgctcct gtaaatattg cagatcggat tccagcgttt ccggagaaat gcaatactgg
     4741 gtttccggca ctggtgcaat gctgtggtac atcagcaccg gcagggaaac accgccggca
     4801 ctgtccaccg ccgcaaagtg ttgtctgcct gcccgcgtca ggagaattgc tgcaagcacc
     4861 agtgccatat caaaggcaat atatcgaagc agttttcgca aattccagct gtggtacata
     4921 gttcttccct ccattttccg atttcatgca gtaccggcat gtgttcatgt gcaatacata
     4981 atcgtgtggt atactatatg aagggttttt cccaaaaata acagagagag gttttttcat
     5041 ggtacaaatg attgcactgg acttggacgg cacactgctc cagccggaca gcaccctgtc
     5101 tgctgccgcg gaagatgctc tgacaaaggc aatttcgcag ggcatctgca ttgttgtagc
     5161 gagtgggcgt gcctttacag cactgccgga aaaaatccgt cattttgctg gcattcgtta
     5221 tgccgtcaca tccaacggag cagcggtttc cgaattgcct gccggaaaac tgctgtgtgc
     5281 atggacattg ccggagcagg cagttctgga tctgctgaaa atgcagcaag cggcaggaac
     5341 ttttctgata gaagccggca taggcggaca gatgtacgca ccggaagaat atctgaacaa
     5401 tccggaaaag taccggcagg cggcaccgct gcaagcatat ttaaaacgaa cacgaaaacc
     5461 agtagccgat atgacagcgt ttttgctgga acatcaacat gaaatagact gcatcgattt
     5521 gatttgtccg gaatgtgcag aaaaagaccg gcttcgtgta caggtgcaac aggaaatcgc
     5581 tgatatttac gtaacatctt ccacaccgga attggtggaa atcgcccatg cagaagcagg
     5641 gaaagccaat gggctgcggt ttctgtcaga acaaaccggc ataccggcag aaaatattct
     5701 ggcatttggc aatgcggaca atgatgcaga aatgcttgcc tatgccggaa tcggtgtcgc
     5761 agtggcaaac gcttccgatg cctgcaaaca agcagcagac cgaatcacct tatcgcacac
     5821 agaagacggc gttgcaaaga caattctgga atatttgcat tgatttttgc aaggaaaaga
     5881 ttgacttttt gccagaattc ttttataata gaagaaatgc tgtatgaagc ggttaagaaa
     5941 acagctcttt ccagcaattc cgtatatata ccggcaatac cgcacaaaga gcgtctggcg
     6001 actttgtacg gtattgccta ccatattttg tggaaaggaa tctcgaaacc gattacaaac
     6061 aaatcggttc gaagaaagaa ggttgaaaat ttctatggaa caaaaacatc gaacatccgt
     6121 cacgctttca acgtcaccac gaaagctgac attgccgggg aaaatactgg cagtctgcct
     6181 gtgtattctc ccgtttctca caaggtcact gacggtagaa atcccacagg cagtgcggtc
     6241 tgcctatttt ctgggagacg gcggctacag cgatttcgaa cagctttttc gggaattttt
     6301 cctgctggga atcgcggctg ccggcgtgat ctggtttttc tatgagcaga ttgcactccg
     6361 cccaaagcga aaattgccaa tgacccgtct tacggcagtg gtatttattc tgctggggct
     6421 ctatttgcta ttgggcttgc tgtccactat tacatcagag tatgcaacag aatcgtggct
     6481 gggcatctac atgttgtacg agggttatct ggcactggta ggttacgtag tcgtgtttgc
     6541 ggcggcatgg tactggatcg accgaaaaga agttttggac tttgtaaaaa gctgtctgac
     6601 tgtactttct attgtactgg gcgtattggc attgctggaa cgctccggca tttgctatta
     6661 caatagtgca atcgtgcaat ttctgggcgg cttgcaggga acagtcggcg ttggcgatgc
     6721 tgtatcactg acatttggca atgcagacta tctcggcatg tactgtgcaa tgcttttacc
     6781 ggtagtagtt tcgctgatta cccgcgaggg aactaccaaa cgtttactgg tacagattct
     6841 ggcagcggtt ctgctggcaa cggcactgtt gctctccaag gtcatgaatg cgattctgct
     6901 gggctttgga ctgacagttg tctttctgct gatctgggca ttccacacca attggaaacc
     6961 gatagtcaaa ggctgccttt gcggtgttac tgccgccgca gtggtttttg gcagtgtagg
     7021 attcctgtgg agccggaacg gtgacacttt gtcagaaaaa ttccggcaca ccatgatcgg
     7081 catggcacag gaggaaacct tccggattct atctatcgat ttgaatggaa acaccgtttc
     7141 cgtaaaaaat gcgaacacca cattcagtgt ggctaccgcg ggaaacaatc tgacaccgca
     7201 ggaactgaca ttcacctgca acggcacaga agtcaccccg caaatcgatg cagacggcat
     7261 gacttgttcc tttgccgaac cggaattgaa acactgtcag gtgcaggtgc agtctgaccg
     7321 actggatttt ctgatgggct atgccacgcc gctggaagtg gtacggcaaa acggcagctg
     7381 gcttgcactg ggcgtaggac aaacgccgct gacagaggta ccggaagtaa gcagttcaaa
     7441 atctcttcag cagagctatc cctatctgaa cgggcgagta tttgtctggg caaacaccat
     7501 ttctgcacta cgggactgct ggattctggg acacggtcct gccaccacaa ttttctatct
     7561 gaatcagtat gacttgccgg cactgttgaa catcttcggt gtgtacgcct tgtacaacaa
     7621 accgcataac tggtatctgc aagtggcaca ggataccgga attccgtcta tgctgctgat
     7681 tctgggtgta ctggtgctgt tctttgtatg cggtttccgg aagtgtttcg gaaagcagga
     7741 aaaatgggac gcattccgca ccggactgct gctctcagtg ttatcttatg cgttaacggc
     7801 atttttcaat gatagcctga tttaccatgc tccaatgttc tggttcctgc tgggaatcgg
     7861 ttggcggcaa atgactgtcg gtacagaaga ataaaacgcc ttttctcaaa cataaaaaaa
     7921 tcccgctgtg catacaatca gcacagcggg attttcaata tttcttttta taaaaactga
     7981 tattacagag tagaaaccga gaggctgtct tccggatcgg gagaaacacc gttgtcggtg
     8041 tacaactcat aagaagcatc agcatatccc agcagccgga gcgtgctcaa atcggtcttt
     8101 gctgtgctct cagccggctc tgcaaacgga attgctttct ggttccggat aaagaacacc
     8161 agttcgtcca gagccaccgg cacataatga tgccccttcg gcaaaatctc tgtttcgtag
     8221 ttgtcgtaag acttcatacg taccagcatc atttcttccg gcaggtaaac ataccgtccc
     8281 tgtgcgttct gcttgtaaat cggagcgatc atcagttcat tgccgaggaa aagctgatcc
     8341 tccacctgtg ctgcttcagc atcttccgga aaatcaaaag caaggggtct gaacatcatt
     8401 tcgtcgtgca aagctgcttt caggaattcg ctgtagagat acggaatcag actatagcga
     8461 atcttgacca tttctccggc agcagcaaca ttgttaaaac gatagagttc ctgttcacgg
     8521 gtgccgtctg cagcatgatt gcggaacagc ggtgtaaaca gagaatactg aagccagcgg
     8581 agcatcagat cttcggtagt atcgcagcca aatccgccag tgtcacagcc agtaaagagg
     8641 aagccagcca tattcagtgc cggcagctgc tgcatagact gaagaatatg ggaccaccaa
     8701 gacttgttgt caccctgcca gatgccaccg taacgatgtg caccaatata agaagaacga
     8761 gagaagaaca gcgtccgctt ttcagggcag atctcctgca atgcctcaaa ggcagagcga
     8821 gtcatattca tgccatacag gttgtgtact tcactgtgct tcaccatttt gccgtctaca
     8881 ttgtgataga acttgtcata gtcaccgata ttgccgttca gtcctgccac catgccagtg
     8941 aatgcgaagt attcctgaat gcccatgttg ccggaagtca gttttttaat ctctgcacaa
     9001 gtgtctgcca gacgatcttc ggtatagaaa attgccggtt cgttcatatc attccagaaa
     9061 ccgtcaatac cctgttctgt cagaacggcg tatttcttac cgaaccactt acgggcatcg
     9121 ggctgcagga agtccggaaa atgcacccga ccaggccata cagcaccgac aaaatcgccg
     9181 ccctctgcat tcttacagaa gtagttgttc tgtacgccct cctcgtaaac atcatagtct
     9241 ttttcaattt ttacaccggc atcgataatc ggaaccagat gaataccctg ctgcttcatg
     9301 tcttccgcaa gagctttcag attcggaaaa cgctcagtgt ccacggtaaa atccttgtag
     9361 cgttccatat agtcaatgtc cagataaatg ctgtccagcg gaataccagc agcctgatac
     9421 cgctttgcga cctcgcgaat gtcctgctcg gtcttataac cccaacggct ctgaccgtaa
     9481 ccgaacgccc agaacggggg aatatagctt ctgccgatga ttttccggaa ctgcttgaca
     9541 atgtcctttt cattttcacc agtgatgata taaactacca gatcattctt tgccgccttg
     9601 atctgcatca ggtcccgccg agtatagccg atgtcaaatt ccatttttcc ggcatagtcg
     9661 aagaatgctc cgaatgtcac atcaccgcga atgagaatga agttgtgaga gccataaaga
     9721 gaacgagtat cctcatggtg gttgggattg tcatagttcc agctcacata ctgccagccc
     9781 cgcttattga taccgcgaat ctgttcgccc agaccgtata caatatcatt ttcagaaagg
     9841 gcatagttaa aagttccctg attgtcagtt tccagaaacg gcagcttgtc ctttgcggtt
     9901 tccatttctg tgacaacagc attggtaggg aacggtgttc cgaatacata ctttttgatc
     9961 ataatggcac ttcctttcaa attatctctt gggcaatacc gcacaaagat tgtgcgacag
    10021 atgcgtcgtc aaatttttcg tc
//
LOCUS       DAWBGR010000087         9901 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_1948_length_9901_cov_5.490453, whole genome shotgun sequence.
ACCESSION   DAWBGR010000087 DAWBGR010000000
VERSION     DAWBGR010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1948_length_9901_cov_5.490453"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5770..5779
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2137 a         2566 c         2921 g         2267 t
ORIGIN      
        1 atttgcaagc agatggcgtg caaagtcgcc agacgctctt tgtgcggtat tgccgcaaag
       61 gcagtcacgc acctgtggag gtgtgcggct gctttttttg tgtgtggatt ctctacttta
      121 agcggtggag gggttctgtg gaatcttttg cgtaatgtgc taggttttac ggaagaatcc
      181 ctcagtcagc ctgcggctgc cagctccctt ttcaagggag ccatttatgg cttaacaaca
      241 taacaaaaac cgccacaccc atgcactttt gcatctgggt atggcggttt tgtaatgact
      301 ggaaacggaa tgttctcacg ttcccgcagg aatcaaacga ttacttgaag tatgcgacac
      361 cgctttcgaa gatcttctga tccttttcgc ccggaacgtt ctggtacagg ttctgtccga
      421 tacgttcgct gtgtcccatt ttgcccagca ctctgccgtc cggagaggta atgccctcga
      481 tcgccatcat ggaagtgttg gggttgaagt gaatgtccat ggtcgggttg ccgtccatgt
      541 ccacgtactg ggtggcgatc tgtccgttct tcgccatgtc tgccagcagt tccggcggag
      601 caacaaagcg tccctcaccg tgggaaatgg ctacggtgtg cacatcgtcc acctcggtgc
      661 cgtacagcca cggggactta ttggaagcga tgcgtgtccg taccagcatg gactggtggc
      721 gggcaatggt gttgaaggtc agcgtcgggg atacatcggt catgtccatg atctcgccga
      781 acggtaccag ccccagcttt accagtgcct ggaagccgtt gcagataccc agcatcagac
      841 cgtcgcggtt cttcagcagg tcgtggacag cgtccttgat gcgggggttg cggaagaacg
      901 ccgtaatgaa cttaccgctg ccctccggct cgtcgccgcc gctgaagccg ccggggatca
      961 tgatgatctg tgcctgacga accattgccg caaaggtggt gatagaatct tccattgccg
     1021 ctgcggacag gttgcggacg ataaaggtct gcggtcttgc accggcacgc tcaaagacgc
     1081 gggcggtgtc gtattcgcag ttggtgccgg ggaataccgg aatcagcacg cggggctgtg
     1141 cactcttgac cgaagcggac agcgtggtct ttctcggata ggacagcttt tccggaacct
     1201 ctgtggtggt cttgatgcgg caggggaata ccggttccag cttcttctcc cagacggaca
     1261 gcagcttgtc cagctggagg gtgaacttcg ggcagacgat ctccggcttt tcgctggtgg
     1321 aaccgatcac gtcctcgccg tcctctgcat cgccgcagag ttccagcagg aagccgccgc
     1381 agcagggctg gaacagttct gcatctgtca gctggcggtc aaaggtgaag ccgatgccgt
     1441 tgccgaagca catcttggca ataccttctg ccacgccgcc gtagtcgatg acccagccgg
     1501 cacgaatgcg gccggcggcg atcattgctt cgaccttgtc gaatgtggca cgaacgctct
     1561 cgaagctggg gataccgttt tcgtcagttt ccggacggag catcacgacc ttgtcgccag
     1621 cctgcttgaa ttcggtggaa accaccttgt ctgccttggc ggtggaaacg gcaaaggata
     1681 ccagcgtcgg cggaacatcg atgttctcga acgtaccgga catggagtcc ttaccgccga
     1741 tagagccgca ggacagttcc agctgtgcct tgaatgcacc cagcagggca gccatgggct
     1801 tgccccaacg cttgggatca ttctgggtac gctcgaagta ttcctggaat gtcagccagc
     1861 agtggtgcca gctgccgccg gctgcaacga ccttggcaat ggaggtgatg actgcgtcca
     1921 tggcacccag ataggggttg cgttcgctga ccgtgggatt atagccccag cccatgagcg
     1981 agcaggtgtt ggtttcgccc ttgagcaccg gaatcttggc agccattgcc tgtgagggtg
     2041 acagctggta gacaccgccg aaaggcatca gcacagtgcc ggcaccaatg gtggagtcga
     2101 acttttcgat cagacccttc tgggagcaga tgttcaggct ggacagcagt tctgtccatg
     2161 cgtctgcact gtcggtgagg gtggattctg ctgccggctg cggtgcttca atggcaatgt
     2221 cggtgtgctt tgcggcaccg ttggagttca ggaactcacg ggacaggtca acaatggtgt
     2281 tgccgttcca gtgcatccgc aggcggggtt ctgcctgtac ggttgccacg atcgtagctt
     2341 ccagattttc cttgccggct tccttgatga acttgtcggc atcttctgcg gcaaccacca
     2401 cagccatacg ttcctgcgat tcgctgatcg cgatctctgt gccgtccaga ccgtcatact
     2461 ttttcggtac ggcgttcagg tcgatctcca gaccgtcggt cagttcgccg atcgcaacgg
     2521 aaacgccgcc tgcaccgaag tcgttgcagc gtttgatcat ctgtgtcaca gtggggttgc
     2581 ggaacagacg ctggagtttg cgttcttccg gagcgttacc cttctggact tctgcaccgc
     2641 agtgacccag agattccacg gtgtgggact tggaagaacc ggtcgcaccg ccgcagccgt
     2701 cacgtccggt cttgccgccc agcagaatga caatatcgcc gggaacaggg gcttctctgc
     2761 ggatattgga agccggagca gcacccagta ccgcaccgat ctccatacgc tttgccatgt
     2821 agccggggtg gtatacctct gccacgtgac cggttgccag accgatctgg ttgccgtagg
     2881 agctgtagcc gttggcagca cccacggtga tctttctctg gggcagcttg ccggcaatgg
     2941 tatcctctac cggcaccagc ggattggctg caccggtgac acgcattgcc tgatatacgt
     3001 aggaacgtcc cgacagcggg tcacggatcg caccgcccag acaggtcgca gcaccgccga
     3061 acggctcgat ctcggtgggg tggttgtggg tttcgttttt gaacatgagg atccagtcct
     3121 cttctttgcc gtcgatgtcc accttgatct tgacggagca ggcgttgatc tcctcgctct
     3181 cgtccagatc gttcagcttg ccgtccgctt tcagcttctt ggcagcgatg gttgccaggt
     3241 ccatgagcgt catgggcttt tcgcctctgc ccagttcctg ccggacggtg cagtatgcgt
     3301 cataggtttc cctgatgtag tcggtgggaa tgtccacgtc ggaaatctgt gtcaggaatg
     3361 tggtgtggcg gcagtggtca gaccagtagg tatcgatcat gcgaacctcg gtaatggtgg
     3421 ggtcgcggtg ttcggtatcg cggaagtatt tctggcagaa ctgaatgtcg ccgagatcca
     3481 ttgccagacc gtagtcagcg atgaactgtt tcagttcgtc ctccgacagg gagatgaagc
     3541 cggtgagggt ttctactgtt gtcggaatgg tatagttggt gtccagtgtt tccggcagtt
     3601 ccggagaagc ttcgcggcgt tccaccggat tgataagata ctttttcaga cggtcgaatt
     3661 ctgcatcggt caggttgccg gtaacgacgt agacatctgc tgtgcggaca cggcagcgtt
     3721 cgccctggag caggatctgg atacactgct cgcagctgtc ggcacgctgg tcgaactgtc
     3781 ccggcaggta ctcggttgcg aatgtccgct gcccttcttc cagagccggc agttctgtgt
     3841 aggtgtgatc caccgccggt tcagaaaata cggtgggaac ggcacgctgg aaagcttctg
     3901 ccgtcagatg atctgcatca tagcggtgga aaatgcgaat gtcggtgaca ttcaggcgga
     3961 gtgcggtctg cacgtcgctc agaacagaac gggcttctac cgcgaattcc ggctttttct
     4021 ccacataaat acgaaaaaca gacatggtaa atctccttta ttttggattt gcgggtgctg
     4081 cgatgttgtg aactggctcc cctttggatt ataggctccc ctttagggca cccgaagggc
     4141 gtgccttgtg ctgagctggc aagccgcagg cttgactgag gggtgcttct gtgaagccta
     4201 gcacatgagg gaaaagattc cacagaaccc ctccaccagc tacgctggtc cccctcccct
     4261 ttcaggggag gctttttgtt caggttttcc tttggattat aggattcccc ttttaaggga
     4321 ggctgatttg gcagactggt tcaagggagc ctgattttgg ggcacacggg gcgatgtggg
     4381 catcgccccc tacagggctg tcctttcgtc aagactggat tctgtcagta tcccatagca
     4441 gcattccgca tcactctcta ctattctaac acaaaaaacg cgattacgca aacttttttt
     4501 tggatttttt gcagaaattc gcacaacggt gccgtttggg gtatgtccca cgtggtagcc
     4561 gtcgccccgt tcccgttcaa aaagtactgg aaattccttg ccgatctggc ttttcaggaa
     4621 gttttccttt gcggttttcg ccgctttctg ggcacgttcc atgcggctgg ttttttcggc
     4681 tccgctgcac tggccgggga actgtgcggc acgggtgccg gctctggggg aatagcggaa
     4741 cacgtgcatg gcggcgaagc ccatgcgttc cgcaaacgcc agcgaggtct gaaagtccgc
     4801 ttcgctctcg gcgggaaagc ccaccatgaa gtccgtggta atggcacagt cggggaagtg
     4861 ccgccggatc ctccggcaga tctccgcgta ttccgcactg tcgtatttcc ggttcatggc
     4921 acgcaaggtc ttgtcacagc cgctttgcag ggacaggtgg aactgggggc agaactgggg
     4981 cagggctgcc agtcgggaaa gctgttcctc ggtcagccgt tccggttcga gagaacccag
     5041 ccggacacgc tccacgccgg cggtgcgggc acagcactcc acagcttctg ccagcgagcc
     5101 gccccactcc atgccgtaga agcccagatt gatgccgcag aggacgatct cccgatagcc
     5161 gtttttcgca aaggcagcgg ctccggcggc aacgtctttc agcgaaaggc tccggcagcg
     5221 tccccgtgcg tagggaatga tgcagtagct gcaaaagcag ttgcacccat cctggatctt
     5281 caggaatgca cgggtgttgt ccggcatggt gtcacagggg agcacctcga aatcgtcctg
     5341 ctgggcgtag ctgtccacgg cggcgatgcg ggcgtggttc tggcacagct gggcgagcag
     5401 tgccggcaga cggctgcggt gcttggtgcc gataatcaga tcggcttcgg ggatcgccgc
     5461 agcctgttcc ggatacgcct gcggatagca gccggtgagc actagcagag catcggggtg
     5521 ttcccgccgg acttttcgca tggcgtggtg taggcggctg tcgccggagg cggtgacggt
     5581 gcaggagttg aacaaaaaga tgtccgcctg ctccggctgg gaaaccacgg tatagccggc
     5641 gttttgcagc agggactgca tgccggcggt ttcacaggtg ttcaccttgc agccgaatgt
     5701 gtaaaaataa cagttcaagt ttgggtgtga cctcctcggg ggaaagatcc ggcagctttt
     5761 tcgacggaan nnnnnnnnnc accggatttt cccgaaatct ttgtgtcatt ttcaccggat
     5821 tttcatgaaa attaacgtca ctgtcaaaaa agcagcggcg atttcatcat ctattatact
     5881 atatcgacga aaatgttgca atcatcttga caagaaaaat ttttcgtggt agactataag
     5941 tacagaaacg gaaggcgtca gaaacttcca aaagaaagca tgatgcggtg tgcacgttgc
     6001 cgtagcgtgg agttcgcttt tcgttctgca atttattgtg acaccggaag ggcgagaggt
     6061 aaccccttca agaggaggta attgttatgt atgaaaccac aattcagctg aatgcaatca
     6121 atgatgtaaa ggatttcgta aacaaggtta tgctgtttaa ctatgacatt gatctggtgt
     6181 ccggtcgtta tgccatcgat gcaaagtcga tcatgggcat tttcagcctg gatctgtcca
     6241 agccgatcaa gctgcaggca cacaccgatg atccggaaaa gctggtagaa tctatcggca
     6301 agtacattgt caagtaagta cagattattt tgagaacaca tatcttttca aagattgagt
     6361 agaatagtaa gaggggaaga cggcactccg gcaacggggt gccgtttttc tgttttgggg
     6421 tctttcttta gggaagcggg caagacgtag gcttgactga aaggtgtcct gatacatcac
     6481 ataaagaaaa agatttcaca aaaaacctct ccaccagctt cgctggtccc cctctccttt
     6541 caggagaggc ttatttccac agttatcaaa aataaaaatt cttaactatt gcagtgtatt
     6601 acagaattga aagtaaggat ctcttttgct atttagcctc ccctgaaagg ggaggggaac
     6661 cgccgttaga cggtggaggg gttctttcgt gaaatctttt ccattccgtt ttagatttca
     6721 cagaagtacc cctcagtcac ctgcggtgcc agctcccctt tcaggggagc ctaaataaca
     6781 aaggggagcc tgaatgaaaa tagcctccct tgaaaaggga gggggaccag cgaagctggt
     6841 ggagggatac tgctgagatc ttttgcataa tgtttcagat ttacgcaaaa atccctcagt
     6901 cagcctgcgg ctgccagctc ccttttcaag ggagcctgaa taacaaaggg ggagcctaat
     6961 tgacaacaga gaaaacccca gtcattccgc cgctgcaaaa caccgcatac ccgcaccctg
     7021 ttcggcatat tttatacaaa atacaaaacg aattcgggag ggtgtgctta tgaaacgtta
     7081 tgcggcgttg ctgtgctgcg gagtgctgtt gttctggtgg ctgctgccgg gaagtccggc
     7141 ggcggtttcg gcggcggagg agccggagat ccctgcggct gcctatgccc tgctggaggc
     7201 agaaaccggc acggtactgc tggaatccca cggcagtgac cggctgccct gcggagcgat
     7261 ggcgaagctg atgacggcat tgctggcggc ggagcagctg gagcaggggg cgtggacaat
     7321 ggacacagtg cttactgcca cagatgcggt gaccggcacc aagggggctg tgatctggct
     7381 caccgccggc gaggaaatga ctgtggggga cttgctgaaa gggctgatcg tggggaacgc
     7441 caacgatgcc gccgttgtgc ttgccgcagc tgccgccggt gacacgaagc agttcgtcat
     7501 ggacatgaac gcccgtgcct ttgatctggg catgcgggac acgtggttca ccagccccca
     7561 ggggaacacc gacaccagct acaccaccgc ggcagatctg ggacggctct gctgtgctct
     7621 ggcgaaaaag acagcgttgc agccctattt ccggacgtgg cgggatttcc tgcggggaga
     7681 agccacggaa ctggtcaacg aaaacacctt ttcccgtacc gacgaaacca gcatcggctt
     7741 caaggcttgt cacagcgaag cggagggctg gagcattgcc gccggtgcca ggcggaacgg
     7801 catgcagtgc gtggcggtgg tgctggggtg cggggacacc gacagccgct tcactctggc
     7861 gaaacagctg ctgcggaagg gcttcgccgg ctggaaagtg gtgcagcccc agctgtccgg
     7921 cgagttcctc tatccggtac aagtccgtgg cggcgtggaa cgggcagtgc tggcggagtc
     7981 ggggccgctg aaaggactgg tggtaccacg cagctgcacg gagctggaaa ccgttatggt
     8041 actgccccgt tttgtgcagg ctcccgtccg gaaagggcag aagctggggc atgccgcgtt
     8101 ctatcagggg gacacgttgt tatatgaaac tgaagtggcg gcggctgatg cagtgccggt
     8161 gcgggacttc tgggacgctc tgtatcaaat aacggtcaaa atgtttaaaa tgtaatcgga
     8221 aaaattgtgt agattataca agaatccaga taccccttgc aatctgccgc agaatatagt
     8281 atgatataag tggtaaagtg gttggacagc acccgtatgt gcagtttctg tcagagcgtt
     8341 cctgcggtca caagggaatc gtctggcgga agcagcgttt ttcgccgtac tgtccaacgc
     8401 cttgagaaga aagtggaggg aaattgtatt atgtcttctg taaagctgaa tacaaaatat
     8461 ctggcaaact atgtgaatcc ggacgaactg accggaatcc gtcatcaggt ggaagcatct
     8521 gcaaagatgc tgcacgaaaa gaacggtctg ggttctgatt ttctgggctg gctgacactg
     8581 ccgaccgact atgacaagga agaattcgca agaattcagg cggctgccaa gaagatccag
     8641 aagaattccg aggtgctggt cgtgatcggt atcggcggtt cttatctggg tgcccgtgcg
     8701 gcgatcgaat tcctgaagtc cccgttctac aacaacctga agaaggacac gccggacatc
     8761 tactatgtgg gcaacaacat caaccccacc tacctgaacg aagtgatctc catctgcgag
     8821 ggcagagatt tctctgtcaa cgtcatttcc aagtccggca cgaccacaga gccggctctg
     8881 gcattccgtg tgttcaagca gctggtagaa aagaagtacg gcaaggacgg tgccaaggat
     8941 cacatctttg caaccacaga taaggcaaag ggcacactga agtccctgtc cgacacagag
     9001 ggctatgaaa ctttcgtggt tccggacgat gtaggcggac gttactccgt tctgactgct
     9061 gtaggtctgc tgccgatcgc agttgccggc tgtgacatcg aagccctgat ggctggtgcc
     9121 gcagaagcac agaaggaact caccgcagat ttcgacaaca acgactgcta caagtatgct
     9181 gcactgcgga acattctgta ccgcaagggc aagaacgtgg aaatgctggt ttcctatgag
     9241 cccagcttcg tgaccatggc agaatggtac aagcagctgt tcggcgaaag cgaaggcaag
     9301 gacaacaagg gcattttccc gtcctccgca gtattctcca ccgacctgca ctctatgggt
     9361 cagtacattc aggaaggcag ccgcattctg ttcgaaaccg ttgtggacat caagaagccg
     9421 aagcaggact tctatctgga accggacgca gagaatctgg acggtctgaa cttcctgacc
     9481 aaccagaaca tgtctgtggt aaaccgcaag gctctggaag gcgttgttct ggcacacacc
     9541 gacggcggcg taccgaatat gatcctcgag gtagaggacg tttctgaaaa gagcctcggc
     9601 tatctgatct acttcttcga aaaggcttgt gcagtttccg gctatctgct gggcgtaaac
     9661 ccgttcaacc agccgggtgt agaaagctac aagaagaaca tgttcgctct gctgggcaag
     9721 ccgggctatg aagaccagaa ggctgctctg gaagcaaagc tggcagaata actggaaaat
     9781 actttaggca ataccgcaca aagattgtgc ggcagatgcg tcgtcaaatt tttcgtcaga
     9841 tgaaacaaaa agcggagtga atacttgatg tattcacgag catttttgtg aaatatgacg
     9901 g
//
LOCUS       DAWBGR010000088         9610 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2006_length_9610_cov_3.922135, whole genome shotgun sequence.
ACCESSION   DAWBGR010000088 DAWBGR010000000
VERSION     DAWBGR010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9610)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9610)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9610
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2006_length_9610_cov_3.922135"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8686..8695
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1955 a         3038 c         2422 g         2185 t
ORIGIN      
        1 aagcagatgg cgtgcaaagt cgccagacgc tctttgtgcg gtattgcctt atatattata
       61 ttttgtgttt gtgctgaaaa tttctccggt tccaattctg tttttaccgg tacagctgat
      121 acagcaacgc attacagatc gccgccgcca cattgctgcc gccctttcgc ccctttgcca
      181 caatatatgg cacctgctcc tgtgccatga tcagttcctt ggcttccacc acgttgacaa
      241 accccaccgg cacgccgatg atcagcttcg gcagcagctt tcctgcacga atcagttcgt
      301 ccagttgtat cagagctgtc ggggcatttc ccactgcaaa aatcaagtcc ccgtccagcc
      361 ggcatgcctt ttccatggaa accattgccc gcgtcacacc acgcactctg gcttcctctg
      421 ccacgtctgc atcggacatg aaacaatacg cttcacaccc cagcccgtgg agtgaacgct
      481 tattgatgcc ggaaagtgcc atattggtat ccgtcacaat gtgtgctccc ctttgcaggg
      541 cttccagtgc agacgacacg gcattttccg aaaagcacag attttccgtg taatcgaaat
      601 cggcagtcgt gtggatcacc cgcttgagaa tgctccgttc cggctccggc agtgttacgc
      661 cggtgaggtg ttcctcgatg atcgccatgc ttctcgcttc aatgtccatg ggtttctgat
      721 agatcggttc cataaaaatt cctccggtac tgctgcaatt gtgctgcgac ctctgcaatg
      781 gtgtgaacgg attcctccgg tgccgtgatc agcaccaccg gaatctgcat ggtctttgct
      841 gctgcaattt tttctgctac gccgccgcgt ttgccgctgt ccttggaaac cagtacagca
      901 atctgatgtt cccgcagcag cttctccgtg tccgcctgcg aaaaaggcgg cttggcgtag
      961 atacaatgtg cctgaaacgg ctccgtcagg cggcgggaat ccggtgtgtc cagaatcctc
     1021 gcccaccccc gcaccgcaaa ttccggcaca ttttccgcat aaaacgaaag ggtatttcca
     1081 ccggtggtca gcaaaatgtt tccggaaatt ccggcgagat aagctgccgc cgcttctttg
     1141 gaatccaccg gcaccagctt gtcataggca tacgtcatct tctgccgtcc cagccgaaaa
     1201 taaggcagct gcaattcggt gcagacctgc cgcacggttt ttgtcacttc ctgtgcaaat
     1261 gggtgggacg catcaaccac ggcatgcata ccgcggagag cctgccggaa cccttccgtg
     1321 tccagccgtc ccacatgcac ggtgcagtgc tgtcccgcca gaatttcttt cccgtatgcc
     1381 gtagcggcaa aagcggtgac agtatcttcc gccggcagtg ccgcgatcag ttccgtagct
     1441 tcgctggttc cggcaatgac agcgatcttc aacgtgattc ctcccgtctg acacaatagc
     1501 cgcggggcgt gatcattttg ccgttctgca cataggtctg ggaattgccc accagcacca
     1561 cacagaacat atccaccggt tcttccggca gcttttccag cgtggtgatc caggcccgtt
     1621 ccccttcccg tcccgcattc cggacaatgc ccaccggtgt ttccggcttg cggtgctgct
     1681 gtatcagctt tgccgcttct gccagatacg ccgtgcggga acgggattta ggattataca
     1741 ggcagatgac aaaatccgcc tccgccgcca gtgcgacccg ccggtagatc tgttccagcg
     1801 gtgtcataca gtcgctcagg ctgataacgg caaagtcgtg catcagtggt gctcccagca
     1861 ctgccgccgc cgcactggca gccgtgacac cggcaacggc agttacttcc acatctgcct
     1921 gcatctccgc tgcaatttcc agcagcaccc cagccatgcc gtaaataccg ctgtcgccgc
     1981 tggaaaccag agctactgtt tttccggtca gtgccgcttc caccgcagca cggcagcggt
     2041 ctgcttcttt ccgcatgggg gtggcataat attgcagttc ggggaactgc ttttccagca
     2101 ggcggacgta tgtggtgtag cccacgacca gatctgcctg tgtcatagcg tccactgcct
     2161 gctgtgtcat atacggacgt tctccgggtc caaacccgac aacataaatt ttcggtttca
     2221 ttgccctcgc tccttccaat gcagcggctc gattgttttc cgacaggcgg caaatgtgac
     2281 accgctgtac ttcacttttc cggtcagcac ctccccgcag tccgatgcca gataggcaga
     2341 agcctctgaa acagaaggca gccctgtgac ctgctgcaca aatgccgatg cagcaaaggg
     2401 atacgcacaa gcctgaatat ctgcatctgc cacgatctcc agcggcaggc ggtacttctc
     2461 acacaatgcc agaatcgccg gctcttcccg cttcagccca atggtcgcca gcttttccac
     2521 tgccgaaaga ctgaggtggt gctcctgcaa aaacgtccgg aagcactgtt ccagatgtgc
     2581 cggcaggatc tgctttttgc accccacccc cagcaccaga cttttcggcc gcagatagag
     2641 cacatggggt gccgcgacag attccgtggt atctgcaatt gccacagcac aggcatgccg
     2701 gggcgtttcg gagaaaggct gtatctgtac cgtttccgga aacgcgtttt tttccaccgg
     2761 aaacgctgtg caaagagaaa ccggctctcc cgccaccaac gccccgctga tatatttcag
     2821 ttcttccaga ttttcgatag tcagatgatt ttccttggca aacagatcga acgccggcac
     2881 gtgttccacg tcagtcgccg tggtgatgac ggcttctccg cccagatacg ccgccgcctg
     2941 ctctgccatg gcattggcac cgcccagatg accggacagc agactgatga catgttttcc
     3001 ccgctgatcc agcaccagca ctgccggatc agatgccttg gactgcacca gcggagcaat
     3061 ggtacgcacc acgatacctg tcgccatgac aaacaccagt gcatcacagt ctgcaaacgc
     3121 ccgccgcact gcttctgcaa aatccgcctt tggttccacc gtgccgccga agtgtgcctg
     3181 caattgttct gccagctgtt tcccctgcgg tgtcagccag aaatacgccg ttttcatgct
     3241 tcggtaccct ttcgaaattc gtgggtaaat gttttatcgt acagcttcga cagggcatag
     3301 gtgtccccca gaaattcgcc cacagtcacc agtgcagtct tggtaatgcc tgccgcctgc
     3361 actttctcgg caatgtccag aagggttccg gtcacgattt tttcctccgg ccagcttgcc
     3421 ttgtacacca ccgccaccgg cgtggctgcc ggataagcag tccgcagccg ttccgccagc
     3481 tccgcaatac gtcccaccga aagaaaaata atcatggtgg cacggtgctt cgccagttct
     3541 tccagccgtt cccgttccgg aaccggtgtt ctgccctcca tgcgggtgag gatcaccgtc
     3601 tgtgtcacgt ccggcagggt gtattccttt tgcattgccg cagccgcagc cagaaacgaa
     3661 ctgactcccg gcaccactgt atgaggaatc cccagcttgt ccaacgcgtc cagctgttcc
     3721 cgaatcgccc cgtaaatgga agcatcgccg gtgtggacgc ggacagtcgt tttcccctgt
     3781 gcttctgttt cctgcatcac ggcaaggact tcttccagtg tcatggcggc actgtcgtac
     3841 acctgacaat ccggcttgcg gtctgccagc accgcgggat tcaccagcga accggcataa
     3901 atgatcgtat cggcgttgtc gatcagccgc ttgcctttga ctgtcagcag ttccggatca
     3961 ccggcaccgg cacctacaaa atataccatt tacagatctc ctcccttgat gagcagtgtg
     4021 gtaaaatacg aatacggctc cagttccggc ttgccgatgt attccgacga catacccaca
     4081 tcgcggagca tggtagtatg ttccagcagt ccccgttccc gcagcagcgg caggagccac
     4141 gccatagcac gtccggcttt catcactacc agattgtcga actgttccag tgcggtttcg
     4201 accgcttcac gggaatgcac tgccggcaga atttgcaggg actcgctgtt ctcgcaaagg
     4261 ctctgctgtg cctttgccgc accggcacaa aaggacggca caccggcgat cacctgtgtg
     4321 gcatagccct tttccgccag aagctgccgc acataggaag ctgtgctgta aacagaaaca
     4381 tcacccagcg tcaccattgc cacctgcctg ccgctgtcca gggctgcaca gaccggctgc
     4441 aatacaccgg aacgaagccg ttcccgatag tcgttgtgtg cctgcatagg aaacagaatg
     4501 tccagccgtt ccgcatggga gaggtctgcc gcctgtgccg caatggcata tgccgcagat
     4561 gtgccgctgg ctttgggaat gggcgtgacg atgatgtctg cctgttccag cactgccttt
     4621 gcccgcagag tcatgtccat cgggtcacct gccccaacgc ctaccgcata aaaaattcct
     4681 tgtttcatgg tttactcctt tgtcatgcac gagaacagca gcaccggatg attgccctcc
     4741 agcacgtgat aatttcccac cgtttttccg ccgctgactg ccagctgcac cacttcgaac
     4801 tgcggcagtt cctgcaacag cggaaacagt tccgcctgcg tttccattgt caccgcactg
     4861 gcgaccagcc gtaccggctg ccgcagctgc cgcagaccgg caaccagctg ttttgccgca
     4921 ccgctgctgc cgcccaaaaa gacacagtcc ggcaccggca aatgctgcac ctgttcttct
     4981 gccctgccgg caatgatctg caaattcttc gtctgaaacc gtgtcttgtt ctgttccaga
     5041 atgtccagtg cctccggctt atactccacc gcgtagacct ccccgaacgg acacatgcgg
     5101 gcacattcta tggaaacgct gcctgttcct gcccccagat cccagacgac cgcgtccggt
     5161 ttcagccgca gcttggacag tatcaccgca cggacttcct ccttggtcat aggcgaacga
     5221 ttccgcagaa atgcactgtc cggcagcagt cccagtctgg tcaccggctt gggagccgga
     5281 ttccgcaccg ccgccacgca cagttccgga tcgtccagtt ctgccgcctg tgccggtgtg
     5341 ccgtgccaga gtttctgggt gggataggtc agatctgccc ccacgaaaat ttccgtatcc
     5401 gacagcccgt atgccagcaa tgccgccgcg atttcccgcg gtccctgcgt gcgggaacaa
     5461 aagaaaaaag tttccgcatg ctccgaaacc gtgcaggcaa tggtgccggc atgatacgcc
     5521 ctgccgtgaa tgctgcaaat cttcgcctgt tccatcgtca gcccaaacgc cgcccccaac
     5581 agctgcagcg atcccacgcc ggggatcacc tgcatttcca gttccggata ccggttccgg
     5641 agcgtccggc aaaggctgta cagcagcggg tcaccggaaa ccacaatgcc gatgctctcc
     5701 cgttccagca ttgccggaag ggattccagc agttcggaaa tatgtcccat aggcatggtc
     5761 tttctgatgt ggagcagttc cagaaaccgc ggagaagcaa tgaccagttc cacttcttcc
     5821 agtgcttttt ttgctgccgg cagcagatac tccgccgtgc cgctgccgcc tccggcaaga
     5881 atcagtttgt gccgcatgtc aaccacgcct ttctgatgga atccgtctgg ctgctctccg
     5941 ccagaatcct gtcattttcg tccagcagca gcagccccac ctgtattttt ccatgggtac
     6001 gccgcatgca gttttcgcag accttctctg caatggtatg ccagacctgc tccgcaaggg
     6061 gataggtaca gagcagttcc gtcatgtccc gtgtggtgtt acactgatac agctgctgca
     6121 cctgtgcacg ggacgctccg gcaagtgccg catgggtgca gatcacttcc cgctgtccgc
     6181 cggcagtgtg actgtgcaga tacataatat ccgctgccgg cttcaccagc tttcccgtcc
     6241 ggcctgccag cagcacatgg gtaaagccca gttcttctgc acagtccagc agatatccca
     6301 gataattgct gcaaagcaca atggcttttc cctgcggata ctgccgcttc agaaatgact
     6361 ctcccgcata gcccgtcaca aaggcacagg cggtgccgta ctcctgccgg cacatggaca
     6421 attccaggta cagggtttcc cgtatggctt cttcgctcat gggacggacg atgccggtgg
     6481 tgcccagtat ggagattccg ccggtaatgc ccagccgtcc gttgaatgtt ttttctgcga
     6541 tcacttcccc gttgggcacg gagatcgtca ccactgcacc cttttctcca atgacagacc
     6601 gcactgcctt ttcgatcatc tgccgcggca ccggattgat tgccggttct cccaccggca
     6661 gtttcagccc ttttcgggtg atcgtcccca caccagttcc ggcacgaaac tgcacggctc
     6721 cggcagcggg aagaatctcc acagaagccg tcaccagaca gccgttggtt tcgtccggat
     6781 cgtcgccgcc gtctttctgc acaccgcagg tataggcttc ttctgcctgt atttccagat
     6841 gcagcagctt tcctgccggc acttccagtt cgacccactc cggacacttg ccggaagtct
     6901 gccacagcac tgaggcaaaa cttgccgccg ccgcacagga accggtcgtg tatccttccc
     6961 gcagctgctt catggcattt tcccccagct gttcagaatg tcgtagattt tcggcatgtc
     7021 cagatgctcc cgcagcagat cagccagctt attgtattcc tgttcctttt tcgccgcaaa
     7081 gtccaccgcc ggcactgtcg ttccggaaat gccctttttc tccaacagca gctgcaccag
     7141 cttctgcgtc agttccggcg tgtcgaaaat gccgtgcaga tatgtgccgc aaactgtgcc
     7201 gtctgcacaa acacagccgt cctttctgcc gtctgtcagc atggcaaaag gctgtgccgt
     7261 ttccgtgtac accgtttcgc ccatgtgaat ttcgtatccc cgcacagcac agccggaaag
     7321 cagttccagc tgtccggaca aacaggaaac cattccggca gtctgagcac gatgcttttc
     7381 cggtcggaaa acggtttcca catcgagcag ccccatgcca cggagttcgc cgccgtcttc
     7441 cacctgttcc ggatcgcaga gccgttttcc cagcatctga aaaccgccgc agatacccat
     7501 gaggaacacg cctttttcgt ggcatttcag cagttccgcc tccagtccgc tttgccgcag
     7561 ccagcggaga tctgccattg tgctcttggt gcctgcaaga aggatcatgt ccggtgtgcc
     7621 cagttcaccg cgtcgggaaa cataccgcac cgacacgccc tccacctgtt ccaacgcttc
     7681 gaaatccgta aagttggaaa tgcggggaaa ccggataacc gccacgtcca gcagagattc
     7741 cgccgccgct tcccgtcggg aaagccgttc ggaaaggctg tcctcatcgt ccagttccaa
     7801 cggcagatac ggcaccacgc ccagcacggg aataccagtc agttcctcca gcatagtcag
     7861 ccccgacttc aggatttcca catctccgcg gaatttgttg atgacaatac cccgaatccg
     7921 gcggcgttcc gcatcgtcca gcagcatgat cgtaccataa agtgatgcaa acacgccgcc
     7981 gcggtcaata tcccccacca gaatcgccgg agaatccgag agttccgcca tgcccagatt
     8041 gacaatgtcc actgacttga gattgatctc cgccggactg ccggcaccct ccagtacgat
     8101 gatgtcgttt tccgctgcca gagcgtgata ggctttcaga atgtagggaa tcagttcctg
     8161 ctttttcgtg taatattcct gtgcacgcat ggtgccatag atttcgccgt tgacgatcac
     8221 ctgcgagccg ctgtcgctgg tgggcttcag caaaatcgga ttcatgcgaa cgtctggtgc
     8281 aatacctgcc gcttccgcct gcatcacctg tgcccgtccc atttccatgc cgtctgcggt
     8341 gatataggaa ttgagtgcca tgttctggga tttgaacggg gctgtccggt agccgtcctg
     8401 ccggaagatc cggcaaagcc ctgccgtgac aaaacttttt ccggcagacg acattgtccc
     8461 cagtaccatt agtgattttg ctgccatagc aagccctcct cgcccagaat gcaccgcaga
     8521 cagtgcagca gatattgatt ttcctccggc agccgcacag ctgcacgata ataagtcgcg
     8581 tccagtccgt cataggtatc gcagtgccgc agcaagattc cgtagggcag cagctgcctg
     8641 tccagatccg gacattgttc cgcctgaaag aacacgtaat tggcannnnn nnnnncccag
     8701 ttttcccagt tcgtcaaaca gccgccaacg ctcattttgc agaaattcca gcgacatttt
     8761 ccgatagtcc tccgccgaca gtgccgcgat gccggcagct tctgccagca gattgaccga
     8821 ccacggctgt ccggtgtgtg cgatttttgc cgtcactttc tgatcggaac agatgccgta
     8881 acccagccga agaccggcaa gggcgtacag tttcgtcagg gatttcagca ccagcacatg
     8941 tggaaactcc gacagccgcg gaatcatgga aacaatgtcc ggctctgttt ccgccatatc
     9001 gcagaagcac tcgtccagca ggacaaagac gtgcttttct gcggcaagat gcaggagctg
     9061 ttccagcagt gccggtggga ttccggttcc tgtggggtta ttgggattac acagcaccag
     9121 aaagtcataa tttccctgcc gcagttcgtc cagcagggca ggcgtgatct gatacggaaa
     9181 cggcatattc cagtgtgtca cgtggcagcc ggcttcgtgg agtgccgctt cgtactctga
     9241 aaatgcagga accggcagca gggcgtgctg cggtttcaga gcatgggcga tgcgaaagat
     9301 cacatcagca ccgccattac cgcagacgat ttccgccggc agtctgccat ggaattccgc
     9361 cagtgcctgc cgcagttttt gctgggcggg atcgggatag tgcaccaatg caccgacagc
     9421 acgctgaacg gcatgctgca ctgtttccgg cacgccgaac gggttgatat ttgccgaaaa
     9481 gtccagccat tcggcggaac attccggatt ttgatagata tttccgccat gtttcggcat
     9541 aaacacacct ccctacggcg agtcgccgct gaccttacgc cccaatttca actgtaaaaa
     9601 gggtagtgtt
//
LOCUS       DAWBGR010000089         9559 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2018_length_9559_cov_4.884785, whole genome shotgun sequence.
ACCESSION   DAWBGR010000089 DAWBGR010000000
VERSION     DAWBGR010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9559)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9559
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2018_length_9559_cov_4.884785"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2234 a         2267 c         2833 g         2225 t
ORIGIN      
        1 aatctgccga aaaatctgac ttacgcatct tacgcacaag ctttattcag tgtttcctaa
       61 aaatcattat aggggaaaaa atttttgggc agtaccgcat aaagccggtg cgtaagatgc
      121 acttccggcc gtcaggcggg tttggtgcag tgctgtcttt gagaaggtac gccgcagaac
      181 gcggtacatt ggcaggaggt catatcgtca tgcagaaaat tggattcgtt attccgtggt
      241 ttggagaaaa tattcccggc ggtgcagaaa tggaactgcg ggaagtcact gcacacttgc
      301 agcaggcagg tatggaagtg gagatcctga ctacatgtgt caaggaattc accgccgact
      361 ggaatgaaaa ctattacgcc gcaggaactg ccgtggtggg aaacatcacg gttcgccggt
      421 ttccggtgcg gcggcgggac actgccgctt ttgaccgcgt gaaccggaag ctgatggacg
      481 gcaagcagat ctctcccaag gaagaacaga tcttcgtgga ggaaatggtc aacagtcccc
      541 agctgtacga gtacatgcgg gacactgccg acgagtatgg tctgtttgtc tttattccgt
      601 atatgttcgg tacgacatac tacggcatgc aggtttgtcc ggaaaagtcg gtgctgatcc
      661 cctgtttcca tgacgaagcc tatgtgtaca tgcggctgtt ccggcaggcg tatgtgaaag
      721 cacggggcat gatctacaat gctatgccgg aaatggaact ggcaaatcgg gtgtacgact
      781 tcaccaccac cgagcagatc tgcatgggta tcggtatgga taccaggatt cagtccgatg
      841 cggaaacgtt ccggaaacag taccatatcg ataagccttt tctcctgtac gccggcagaa
      901 aggacgtggg caaaaacgtc cacactttgc tgcggtactt tgctgaatgg aaacagcggc
      961 agaaggacga ccttcagctg gtgctgatcg gcggcggcga tattgccatt ccggaatcgg
     1021 tgaaagacga tgtctatgac ttgggcttcg tttccaagca agacaagtac aacgccatgg
     1081 cagcggcagc actgctctgt cagccctctc ataacgaaag cttttcgctg gtcattatgg
     1141 aaagctggct ctgcggcaga ccggtgctgg tttcgtccca gtgtgccgtg acaaaggatt
     1201 tcgccaagcg ttccaacggc ggtctgtatt tcaaggatta ctttgaattc gagggctgtg
     1261 tgcagtacat cctgcaaaat ccggaggcag ccgcagaact gggtgccaac ggtggggcgt
     1321 ttgtacggga aaacttcgag tgggacgtga tcgtggaaaa ataccggaca tttttccaga
     1381 agctgatggg agcgtgagag catgaagcga atcgcactgg ttcacccgcg gtacgggctg
     1441 gagatcagcg gcggcagcga aacctatgcc cgaaaaattg cagaacatct ggcgaaccgg
     1501 tatgaagtgg aggtgctgac caccaaggca gtggacagtg ccacatggaa agactggtat
     1561 gtgcgggacg tggagatgat tcgcggtgtc acagtacgcc ggttttccgt acagcagatg
     1621 cgtgccagag attttcaggc attcgacgag gcatatctgg cagaactggc acagggcaga
     1681 tccaatctgg tgacggagaa agagtggttc gaaaaacacg gtccctatgc accggctctg
     1741 atccgttatt tgcagcggta tcgggaaacc tatggggcga tcctgtttct gtccgatgcc
     1801 aactatctga cttatgccgg actgccgctg gcgggaaatc gggcgatttt tatccctatg
     1861 gcacaggata ccccgttcct gcggttttcc acactggaaa gcctgtacca gaagccgcgg
     1921 gcgtttgtct ttttgtcgga ggaagaacgc ctgctggttc gccggcagtt tccgctgtca
     1981 gagccgattc cctgtgccgt aatgggactg ggcatggacg tgccgcggca ggtggacacc
     2041 ggcacattcc ggcggcagta cgggctggac ggcgaatatc tgctctatgt ggggcaggta
     2101 gacgaaaaaa aggagtgccc catgctgatg cggtacttca tggagttcca gaagcggacc
     2161 ggcagcaagc tgaagctggt gctggcggga aagaagatct gccggattcc ggagcatccg
     2221 gatattctgg cactgggctt tttgccggag aacgaaaagt tcagtgccat tgccggagcc
     2281 aaggccgtgg tgattccgtc ccgacagatg ggcgtttcca tgacgctgct ggaatctatg
     2341 gcaatgtctg tgccggtgct ggtcaacgga ggaagtccgg tgctgcaggg acactgcaaa
     2401 aagagcaacg gcggcttgtt ctatcagagt tattttgagt ttgagggtgc cctgcaatat
     2461 ctgatgcagc accccgtgga gtatatgcag ctgtgtgcca atgcgagggc atacatcacg
     2521 gcatattacg attgggagag tatcatgcag cgttttcagc agatcataga gtgggtcagt
     2581 ccggagaaag actgacgcgg aaaacgcagt ggaggaagaa gaaaaaggga tgagtttatt
     2641 tacaggagca gtgctgctgg gggttgtact ggcggcactg acagttggtg tctggtggaa
     2701 aaagaccaat gtgctggaac ttttggtgct gggcgtggtt tactggctct gcacatgggt
     2761 gatttccggc atggggcttt ttgtactgga tcagttttcg ctgcggcgtt gtgtcattgc
     2821 agcagtttct gtggaactgc tggtaggcat tgcggcagta ttggtacgaa agctgcggga
     2881 caagaccccg tggaaacagc tggtgcggat ttcatggaac gtgcgtgcct actggattcc
     2941 tgcggtggtg cttgccggcg gttttgtgct ggtggcattc aagcatgagc tgtttggcat
     3001 gggacaggac gagggcgtgt atcagacggt ggcggtcaac ttcctgaacg gcatcacaga
     3061 ccgccagcag gactttgcgg aatatcatac gctttccaag gagcagcagg aattgtttca
     3121 cgacaaggtg ttttcctact tggtgggata cgacttggaa acagaggatt atcccgctac
     3181 agtctatgac cgcaatgtca gtccggtttc cggcatctat cacggaatcc ccacctatgc
     3241 atcgatgctg gcaatgtggg gaaaaatctt cggtatggca aatatgcagg gggtacagac
     3301 cttatgctat ggctgtacta tttttttgtt gttttttctt tgccaaaatt tgaaaattcg
     3361 gaaaagcagc agtgcacttg cctgtgcagt attcgcggct tcgccaatcg tggtctgggt
     3421 ggaaaagtcc gctctgacgg aaagttttct ggcggtgctg ctggtattgt tcctctattt
     3481 gctgacagat gaggaacacc cttatcggca atggctttcg atcattccca ttgctgtttt
     3541 ttcgtgcttt cacgtgtcga tttataccat gataccgctg tttgtggtgc tttatggcgg
     3601 catgttctgg atcacccgcc ggaaaagctt tgcgattctc atgccggtga cacttgtggg
     3661 ctattgcatc agctttttcg ccatgcggca tgtccagccg ttctacacca tgaacaacta
     3721 ccgcccgttt ttcgtggcag ggatctcccc gaaagatctg ccggtgctgg tgccgctgct
     3781 gtgcggcgtg gggctggtgg ctgtgggaat ctacctgtgt ctggtctatt tcctgatctg
     3841 ccggaaaaat cgggacactt ccacggaagt ctatatgcag tgggtgcagc actccaaaat
     3901 tgcagtggtg ctggtagaat tcctgttggt ggcactggtg ctgtacatcg gtgcaaaggt
     3961 gcttaccgca gaaaatccat tgctgaaatt gcaggggaac accctgtggg gatttgtctg
     4021 tggttcgggc ttgttcctgc tgcccattgc gtgggtagtg ggtgtgattt gcccgaaaac
     4081 ctatctggaa acgcccaagc atctggtgat actggtagcc gcatgttact gcgtggtggt
     4141 gtactctgca tttttgcgga cagaggtgga attctactat tattatggtc gctatctggc
     4201 accgtttatg cccattgccg ccgtgtttgc ggcactgaca ctggacaaac tccgtgcgag
     4261 agtgacgctg ccggtgatga cggcatgcct gctgtttgtt tcgccctttg actattttct
     4321 ggcgacgcat caggacgaca cccgcatgac atggtcggtg ctggaggact tgtccaatga
     4381 gatcgattct gatgactgcg tgatcgtcgg cgacgacgac atgtggacac tgtatctgac
     4441 gctgcgtgcc accaccggtg cggcaatgta tccggaaatg gaagatccgg aaacacaggc
     4501 ggcagaactg gctgagcagt atgaccatgt gtactatctg gggaatggca agtggaacga
     4561 ttcctttgag catcagttcc agcaggtata caccaatacc attacccaga gcacagatga
     4621 aaactacagc aataccggca gacgcatcat catgttcccg ctgtcctatc aggaagtcag
     4681 ctatacggtt tccctgtatg cgtactgggg cgaggaacag aacctgtcct atccggcaag
     4741 cacctgtgcc aatacggcat actttgggtt ctatccgctg gaagggaact tctgctggtc
     4801 gggcagcgag aacgcttccg tgcagtgtga cctgcaaaag cgggattaca atatgacggt
     4861 ttcgctgggc ggagccgtgc cgctgtacca gatccaccag aatccgtatg tggtgcatgt
     4921 gttggtaaac ggcaacgacg ttggaacgat cacgctggac gaccagcaaa acgacggtgc
     4981 gtatacgctg tcggtgtcca aggaagtcct gcacgacggc tcgaacattg tcacattcca
     5041 gtcgaaactc tggaagggca gctgtgtgat ggaggacgat gaacgggagc tgggaatccc
     5101 gttgcaggat gtggaattcg aagcggcata agcggctctg aaatatggag aaagggaaga
     5161 agtatatgaa actgatcatt caggtaccat gttacaatga ggagaaaaca ctggaactgg
     5221 cgtacaatga cctgccccgt cacattgacg gcatcgatac cattgagtat ctgattatca
     5281 acgacggcag caaggacaag acggtacagc gggcgagaga actgggattt caccatgtcg
     5341 tgtcgttccg gcggaacaag ggtctggcac ggggctttat ggctggtctg gacgcttgcc
     5401 tgcggctggg tgcggacatt atcgtcaaca ccgatgcaga caaccagtac tgcggcgagg
     5461 acatcgaaaa gctggtgcgt ccgattctgg atcgcaaggc ggatatggtc atcggcgaac
     5521 gtcccattga cgaaaccgaa cacttttcgt ggaaaaagaa gaaattccag catctgggca
     5581 gctgggtggt acgggtggct tcgaacactg acgtaccgga cgcaccgtcc ggctttcggg
     5641 catattccag agaaactgcc atgcgaatga acgtggtcaa cgagtatacc tacacgctgg
     5701 aaacgattat tcaggcggga cagagcaaaa tggcagtggc ttcggtgccg atccgcacca
     5761 atccggaaac ccgcccctcc cgtctgtttt ccagcatgtg gcggtacatg aagcgttccg
     5821 catcggtgat cattcgctct acggcaatgt accgcccgct gcatttcttc ggcgttctgg
     5881 gaacgatcct gttcctgctg ggcgttgctg tgggagttcg ttttctggtg tacttctgca
     5941 tgggcggcgg acacggccat gtgcagtcgc tgattctggc atcgtccctg atgatgatcg
     6001 gattccagac cattaccatt ggtctgctgg gggatactgt ggcagccaac cggaagctgc
     6061 tggaagatgt gcagtatcac gtgcggaaaa tggactatga cgatcatccg gaagccaaaa
     6121 cgccggacga caagcccttg ccggagcagg cagaacagaa ataaaacatt tttgagcggt
     6181 gctgcaaaag caacaccgct tttgtttgtg agatgtgctg aatttgcaga gaaaagtttg
     6241 tgtaatatca cgtgttggaa aggttgacaa atcaagaaaa aaccattata ataaaattag
     6301 gtaaatggct gttaaaacga cagagattta tcacattttc aaccagaaag gggttttatt
     6361 tatgaagatc agaaagggta tttccgcggt acttgcaggt gtcatggcac tggcggcatt
     6421 gccggtatct gccatgagtg caagtgctga gggagcgtat gcactgggtg acgtagatat
     6481 ggacggtttc atcaccggcc acgatgcggc tatggtgaca catgcactgt atgtggacaa
     6541 tgaagcaatg accgcagaac agttaaagct ggcagatgta aacggtgacg gtgttgttga
     6601 ccagagcgat gcagactgga tccatgagaa tcaggtgtat gaactgggaa agactgataa
     6661 aagatttcga atgaatgcga tgacggttta ttatcaggcg tattgtattg ccgcagacgg
     6721 tactggtaat aatggagaat tgcagtactc tttggaaaag ggtgcggatc tttgggaagg
     6781 cggttcttac gacgacaaag tgttgccgga agtctgctac aacctgctgg acgtgaatgg
     6841 tgacggtgtt ctgactgttg acgatatcct tctcacggcg tttgatgtat gttacatggg
     6901 ctgtggtgca agcaagacta tctatttcga agaaggtcgc tatgatctta cagaagatac
     6961 cattccggta gatgactatc agccggcacg aatcaacatg ggataatcaa tatttttcac
     7021 agaaactcaa acagccgtcg gcgacattgc cggcggctgt tttgcatctg cggaaaagca
     7081 cttgaaaaca ggcagcttct gtggtataat agagtataat caataaagca taatcaaaag
     7141 taggagtgtg atactatgaa ggtggttttg ctggcacata cccccacgcc ggaacagacc
     7201 gttgccgctg cggcacggct atgttattct gacaccgatg tagaggctct gcgggagtcc
     7261 atttcgcagg agcgtgcaga acagtttgtg gaaatgctgg cgggattcgg tcacgaaagc
     7321 cccgtggaac atgtgacatt tacatttggg atcgagggcg tttcccgttc gtttctggca
     7381 caggtgaccc gtcaccgcat tgcttcgttc tctgtgcaga gccagcggta tgtgcggcag
     7441 gatcactttg tctttgtgac accgccggca attgttgccg agccggaact gctgcgggaa
     7501 tacgaaaacg ccatgcagca gtccattgac agctacaacc ggctggcgga cgcgttggcg
     7561 gcgaaatatg tgccggaatt tctggcacag ggctgcaccg aaaaggcagc ccgcaccaag
     7621 gcggagaaaa aggcgatcga ggacgcacgg tatgtgctgc ctaacgcctg tgaaaccaaa
     7681 atgatcctga ccatgaatgt ccgtagtctg cggaactttt tccggctgcg gtgctgcaat
     7741 cgggcacagt gggagattcg ggaagtggca acggaaatgc tccggctctg ctgtgaggtt
     7801 tctcctgccc tgttccgcac cgccggtccg tcctgctact gcggcggctg taccgagggc
     7861 aagatgagct gcggcaaggc gaaggaagta cgggaaaaat ttgaggtgat ccatggtgaa
     7921 acaaggtaaa attatcgtgc tggacgggct ggacggctgc ggaaagtcca cccagtttgc
     7981 cgcactgggc aagctgctga cggagcaggg cgaaacggta aagccgatca gctttcccat
     8041 gtatgacaag ccctctgccg cactggtgaa aatgtatctc cgcggcgatt tttccgacac
     8101 gccggacggc gtgaacgcct atgccgcttc cagcttctac gccgtagacc gctacgccaa
     8161 ctataagctg gactgggaaa agaactacac ggctggggaa ctgatcctcg ccagccgcta
     8221 taccacgtcc aacgccattc accagatgag caagctgccg caggagcagt gggacgggta
     8281 tctggaatgg ctggaggatt atgagtactg taaactggga ctgccccagc cgaatctggt
     8341 gctgtttctg tccctgccgt tggaactttc ccagaagctt ctcgccaaac gctatgacgg
     8401 ggacgcttcg aaaaaagaca ttcacgaggc agaccttgcc tatctggaac agtgtgaccg
     8461 tgctgcccgc tatgccggcg aaaagctggg gtggcagttc gtggaggttt cagacggcag
     8521 acagctttat ccggtagaac agattcggga cgaattatac cgacgcatac aggaggcatt
     8581 tgcatgctga atttccggca gatcactctg gcagaccgtc cgtggatcca gacggctctg
     8641 cggcagtccg atttcatggg gtgcgaatac agcttcgcca acaatctggc atggtgccgc
     8701 ggggcggatt cccgcatctg tgcctttgag gggttctatc tgatctgtgc attcgatacg
     8761 ccggacggca cgccgcattt ttcgttcccc agcggaacgg gcgacctgaa acaggtgatc
     8821 gcggcaatgg cggcatacgc ggcggcaatg cagaaaccgc tggtgattac tggcctgacc
     8881 cagcagagcc tgccgctgtt gcaggcggta tttccggaac agttcacgct ggaaaacgac
     8941 cgcgacagtg cggactatct ctatcgcacc gccgactttg tggcactctc cggaaagaag
     9001 tatcacaaaa agcgaaatca tattgcccag tttgccaggt atgctccggt tttctcggag
     9061 atgacaccgg acgactttgc cgagtgcatt gcctttgcag cggaaagcta taacgccaag
     9121 gacggctata ccgatgcttc cagcgtggcg gagcagtacg ccattcacac ctttttcgag
     9181 aacttttccg cactggaact gaaaggcggc gtgttgcggg tagacggcag gctggcggcg
     9241 ttctccatcg gggaacccat ttgcagcaat gtgttcggcg tgcacattga aaaggcggac
     9301 acccgctatc atggggcgta tccggcaatg gcacgggaat tcgcggcaca ctttgccatg
     9361 gactacacct atctgaatcg ggaggaagat ctgggcattg ccggactgcg gaaatcgaag
     9421 ctgtcctact atccggaact gctgctggaa aaatggacgg caacttgtca tacgccggag
     9481 gaactgtgct gaaagccgtt tcttttgggc aataccgcac aaagagcgtc tggcgacttt
     9541 gcacgccatc tgcttgcaa
//
LOCUS       DAWBGR010000090         9466 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2039_length_9466_cov_3.183084, whole genome shotgun sequence.
ACCESSION   DAWBGR010000090 DAWBGR010000000
VERSION     DAWBGR010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9466)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9466
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2039_length_9466_cov_3.183084"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    250..259
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    737..746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8052..8061
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2655 a         2111 c         2116 g         2554 t
ORIGIN      
        1 caaaatcatc ggaatacagt cccgtacccc atgcactcat aattcgtttc tcccttttac
       61 tgtcgtattg ccctaaacat cctcatatga tcttttataa tctgatccgg taaatcaatc
      121 caattatcca tacccaacat attgacacta tcaaatatat tttctaaatg tatcccataa
      181 tcatcttctt ttgctataat tgcagtattc cccacaaaaa caacattttt tgcatttttg
      241 gtgtttaggn nnnnnnnnna cttccatata acaccatctg aagcttcttc aatatttcca
      301 ccatcatcaa cgggaatata ttcagcaaaa tttccaataa aacgaatgct tttaggaacg
      361 gagcgtatag aagatgcaat cataaaataa ccgtaattat aatttccatc attccttgga
      421 atactactcc atttcacaca tgttgttttg ggaggaaacg ttaactcctc tgcaatccaa
      481 cagcgaacca caggatactc atcgacattc tttttcattt ttctctttgc agcaacttga
      541 aatataataa attttccatg taacccgttt tcttttgcaa gattgctaat caatggataa
      601 gcaaacacat caccaatatt ccaggggcac tcatagaatt tctttatccg aatcggtttt
      661 gcctctggct gcggtgaaag taatttagcc tttaattttt ccaaaactgt ttttcttttc
      721 tttcccagag cagatgnnnn nnnnnnataa ttcttgcata gacatgaact ttatcatatc
      781 tacttaaaag taatcgatcc ttgcaaaaat catttaatcc aggtttttct ctcgactctg
      841 ttaacataac acgaactatc ggatatgtac caattgtatt aataaacatt ttctctgcaa
      901 cctgaaatat cgcataagtt ccagcaagac ctgttttttc tgccagttct ccggttaatg
      961 caaacgcata aacatcccca atattccacg gacattcata aagcttcttc actcgaattg
     1021 gtttcgcttc cggctgtgga gattgcaatt ttaattttag cttttccaga actacctttc
     1081 gctttctgcc caaagatggt gattcttctg cccagcgtgc aatatcggtt tcactttcaa
     1141 ggtattccag tgctgtctgt ttaatatcct cgtctaagcg gccttttttc cataaaatgt
     1201 ccgcacatac aaaccaaaat actggtatgt ccggatcgtc attatatgca ttatccacat
     1261 atgttttctt taactcctca aatgcttctc gatatggctt tccagaattg attgcatcta
     1321 aaaattcact tttcacatcc agtgcaaaat catcggaata cagtcccgca ccccatgcac
     1381 ccataattcg ttcctccctt ttactgtcgt gttgatttta ttcccttatg gctcgcctga
     1441 aaaatccgta tatttgtcag acagtcctat tccacttttc cgatcaatgg caatcttaca
     1501 gcgaaagtcg ttcctttttc tgaacttgat tccacacaga cattcccgcc atgcaatact
     1561 gcaatttctt tcacaagggc aagccctaaa ccgacaccgc ccatttgcct gcttcttgat
     1621 ttatccacac gaaaaaaagg ctgaaaaata ctctctctgt atgcttccgg aatcccacag
     1681 cctgtgtctg taatatgcag caatgcctga tgctgttcct tccggataga aaccgttacg
     1741 ctgccgcctg tatgattgta tttgatcgca ttctcaatga gattaaacag cagtctgtaa
     1801 atcagcgtat cactgccgat catatgaacg tcctcacagt tttgatgcag tgtaatattt
     1861 ttctgttccg caagtgtggt gagatccgtc aggatctcgt caaccagcgg tgccaattcg
     1921 atttcatcgg aacgtgaaac agactgcaaa ttgctcattt ccagaagcgt gcggacaagg
     1981 gcagtcaggc ggtttacctg atcgatttga aattgaacca gttctgcggt ttctgcacag
     2041 ctgtcagaat gttcttcggc gaacagttcc aattttgtct gcatcattgc caacggtgta
     2101 cgcaattcat gtgcagcatt tcctgcaaac tgacgctgca agtcgaagga aagtgccagc
     2161 cggtcaagca tatgattgac cgagcagctc agctgctgaa attctgctgc ggtgttttca
     2221 tcaaaacggg tgctggtaag attgtcctga ttgatgtttt ctgtctgctt tgaaaattgt
     2281 ttcagcggtt tgagtgctct gccgcttaca aaatatgcga tcgctgcact aagcagtgtg
     2341 actgcggctg tgatatacca gcattttctt ccgaattctg ctttcacatc gtaaatttcc
     2401 tgcgaaaact gatggagaaa tgcggacatc tgctgatcat caaattcaat tttcagaaca
     2461 tcggaacttt ctggatcata ttccatgaca aagccattca gcgaatctat gccgaccgta
     2521 ccggaacgat agagcagaag attggaacat atacaagttc cggcaatcaa aatggcagtc
     2581 agcagtgtga ttctccattg aaaggacagt cgtttcattc gcttcatttt gattctcctt
     2641 ctatttcata accctctccg attcgattcc gaatggggtc atagccaaga actgcacgga
     2701 gttttttccg gagtgcagaa atgtggacac gaatcgaatt gctgaaactg tccacgctgc
     2761 tgtcccatac atgttcaatg agttcctcct gactgacggg tctgccctga tgcagcatca
     2821 gatattccag tacgccggtt tctttgcgtg tcagattgat tacaacgccg tctgctgttg
     2881 cagtacgact tttggtgtcg tatacgatct gtccgcattg caggcgtaca tcccgctgaa
     2941 tgaactgacg gcgtgtcagg ctgcgaattc tggcttccag ttctgcaagg tgaaatggct
     3001 tcgaaaggta atcatttgcc cctgcatcca gtccctccac tttgtcagca atttcgcttc
     3061 gtgcggataa gatcaatacc ggcgtttcct gattttcttt ccggaagctg cggagaagcg
     3121 tcattccatc gatcttagga aggttcaggt ctaataatat caaatcataa tattccacag
     3181 acagatattc tgaagctgtt tctccatcgc cgcaggcatc gacttcatat cctgaacgct
     3241 gtaatttttt tgtgatgtcc tcccgcaggg attgttcatc ttctatcacg aaaagtctca
     3301 tagcatgctc cccagagatt gttctgtatc ttttgctgca cggtttaccg ttaaaagcct
     3361 tgccatggag cagtgttcgg ggcaggatac aaaacaggct cttataattt ttacctttat
     3421 tataacagac gcagataaaa tctgtgttaa ttttctctta acgcagattt tatatcgcat
     3481 gatgtatact ggtgtcatca aagcattgga gggaaacaaa tggtattcaa aaaagcggca
     3541 gtgcaagtgc tgcttcttct tgcgggaata gcgatgctca tctttggcat ctggcgggag
     3601 gaagcccaga cagttttttc aaaagcgatc cgcctgtgta tggagtgtgt tggaattggc
     3661 taagaacaaa cagatcaaca aaaaaaatgt gcacattctt gcacggtttc gcggttggat
     3721 tcaggcagca gcagcacttc tgaccaacat gcatctcccc aattttttga atggttctct
     3781 gaatcgggga aagggaaaat acgcctgtgt gccgggattg aattgctatt cctgtccggg
     3841 agcagcaggc tcttgtccca tcggggcatt tcaggcagtt gtagggtcat cgaaattcaa
     3901 gttttcttac tatgtcaccg gaatattgat attgcttggt gttttgctgg ggagatttat
     3961 ctgtggattt ctgtgtccgt tcggctggtt tcaggagctt ttgcataaga tcccgtcgcc
     4021 aaagctctct acccaaaagc tgaaaccgtt gcgttatctg aaatatggta ttttgctggt
     4081 gatggtgatt cttctcccga tggtagtcgt caatgagctg ggaatggggg atccgttctt
     4141 ctgcaaatac ctttgtccgc aaggcgtgct ggaaggtgcg attccgttgt cgatcaccaa
     4201 tgaaggaatc cgcacggcac ttggtacact tttttcatgg aaagcatttg tcctgctggc
     4261 tgtgatactt ggcagtgtgt ttttctaccg cccgttctgc aaatggctgt gtccgctggg
     4321 agcattttat gcacttttga atcgggtatc gctttttcag atgaaggtgg acaaagacaa
     4381 atgcgtatcc tgcggagctt gtgcaaaggc atgcaaaatg gatgtcgatg tcaccagaca
     4441 gcccaaccat gccgagtgta tccgctgcgg catgtgtatg aaatcctgtc cgacccatgc
     4501 catatgttat caatatggtt ttggagatct atcaaaggca aaagaaattt caacagaaaa
     4561 aaaggagctt aaaaaatgaa aatgaaaaag aatctgatga tgatgttagc agcttgtctg
     4621 gcactttctg tcccgctcag cggatgcggc agtacggtaa aggacacttc ttcaagcgtg
     4681 tccagtgaag tatccggcga agcagaaaca tccggaaaat cttcacagga acttttggat
     4741 gaggaaaatg cgatcatggc gaaaaacaat gatctgtggg aaaaggtgtt tgcatctatg
     4801 agcaaaaatg tcacagagga cacgctgagt tcaaactacg gcgatgtgct gatggctgct
     4861 gtagacggtg caaaagatca gtttactgat gcagaatatg aaacgctgaa atcagatgca
     4921 gaacagatcc gcaaaattga agaggagatc gcagcacttc cgacggattc ttctgaggag
     4981 cagaccagcg atgcgtctgg cactgcattc ccgcagtttg agggaaaaga tctggacgga
     5041 aataaggtgg acagcagtgt tttcgctgag aatgctgtga cagtggtgaa tttctggttc
     5101 aacgaatgca aaccctgtgt ggaggagctc agtgaaatga atgccctgaa tgatcgcatc
     5161 aaagaacagg gcggtgaagt gcttggtgta aatgtcggtg cacttgacgg tgacgagcag
     5221 aatattgcga cggcgaaaca aattctggaa acaaagggag cgaaatatcg gaacatctat
     5281 tttgattctg acagcgatgc cggaaaattt gcactgggtg tcactgcgtt cccgacaacg
     5341 tatgtcattg acagaaacgg caatatcgtg ggcgaagcac ttatgggcgg cattgacaat
     5401 gacgaaaatc tcagtacact tcagaatacc attgatgagg ttctcgcaaa agatgctcaa
     5461 aagtaaaggc aataccgcac aaagcctgtg cataagatgc gtcgacaaat ttttcggcag
     5521 attgcataaa gttccgcaga tatacattta cacaagatca tatgacaata atgcaaaagt
     5581 acatgacaaa aatgatatac accgcaatgt gaaattgcta taatagacac aacaagcaaa
     5641 acggcacatt tcacaggagg gaatgtatat gaatttgaga aaagtcgtag ctggttttac
     5701 ggcagcactg ctgtctgtaa caacagcatc catttttccg acgaaaacag tttctgccgc
     5761 tgacgtttgt gtcattgata cagacacaga gtatcagacg attcgtggat tcggcggaat
     5821 caaccatccg gaatgggcag gagatctgac gcaggcacag cggcagacgg catttggaaa
     5881 cggagagaat gaactggggc tgacggtact tcgggtgttt gtaaatccgg acagcagcca
     5941 gtggaacaga gcactcccga ctgcacagtt tgcgacgcaa atgggtgtca ctgtttttgc
     6001 atcaccgtgg gaaccgccgg caaatctaac ggaatccggc ggaagcaacg gtaagctgca
     6061 tctgcccaaa tccaattatg ctgcctatgc aaagcatctg aatgattttg gcacttacat
     6121 gaaaaataat aatgttgact tgtacgctgt ctctgtacag aatgaaccgg attatgcgtc
     6181 cgagtggaca tattggtcta ctgatgaaac aacagacttc attgccaact atggcgatca
     6241 gatcacaagc acccgcctga tgtcaccgga atcttttcag tatgtaccgg aaaatgcctc
     6301 atgggtgccg gacggcggca agaaatttta ccggaaaatc ttgaataaca gcaaggcaat
     6361 ggcaaactgt gacttgtttg gtacacattt ttacggcacg cagcgttcgt ggatggactt
     6421 tccggatctg gagaacagcg gcaaagaaat ctggatgaca gaagtctatg ttccaaacag
     6481 tgacaaagat tcggcaaatc gctatccaga ggcactgcag gtttcggaaa atatccataa
     6541 tgccatggtt gtgggcaata tgagtgccta tacctggtgg tatatccgcc ggaattatgg
     6601 tttgatgacg gaagacggaa agatcagcaa gcgtgggtac tgcatggcac aatattccaa
     6661 atatgttcgt cccggtgatg tgcggatcga tgctacggaa cagccgacag acaatgtcta
     6721 tgtgtctgca tataagggtg atgacaatca agtgacgatc gttgccatta acaagggaac
     6781 agaaagctat tcgcagcagt ttgctgtaga tgctgatgca caaatttcag aggttgaccg
     6841 ttatcgtact tcttcctctg aaaatctggc aaaaacagga aatatggagc atgacagcag
     6901 cagcttctgg gcacagctcc ctgcggaaag tgtttctacg tttgttgtga cattggaaga
     6961 ccagccggta gaaccggatg aaaacggtta ttattttcac gatacttttg agtcggacaa
     7021 ctgcgactgg cagggacacg gtgcagcaga tattgcactg agcggaagaa taccgtatct
     7081 gggaaccaac gcactcttgg tgcagaatcg taccagtgcg tggaacgggg cagaaaaggt
     7141 actgccggca aaagcatttc aggctggaaa agaatatagt ttcagcgtat gcctgaatta
     7201 catggacgga gaaagtacta aaaatactgc actttctcta cagtacacag atgctgccgg
     7261 agaaacgaag tacgccagaa ttgcgtctgc ttctgcagca aaggaaaatt atgtgcagct
     7321 cgcaaatccc agtttcaaac tgccggaggg cggaaaagat ttcaagatct atgtagaagt
     7381 cgaaggcgat acagataatt tctatatcga tgaggcgatc ggtgccgtga aaggaactgt
     7441 gatcgacgga ccgcctgtgg taaccaccac aacgacgaca acaacaacta ctactaccac
     7501 taccaccaca aaggcaacga ccacgacaac gaccacaaag gcaacgacca cgactacaaa
     7561 ggcaacggta acgaccacaa aggcgaccac cgcaacttct gcttcgacga caacaacggc
     7621 aaccacgaca gtgataacag aaaaaccgcc tgtaaatacc ggctacatca atgcggacgg
     7681 caaggtaaat ctggcagatg ttgtgctttt gcaaaagtgg ctgcttggcg ttccggaaac
     7741 aaagcttgct gactggcagg caggggatct gcatgcagac ggtgtgctga atggtttcga
     7801 cctttgtctg ctccggcgta cagtgacatc ttccggcaat atctgacgat gcagtttaca
     7861 gatgtgtttt gtgcatcgtt gctctgaatt atgacatttt gtttcatctg atttcctata
     7921 aaaacccgag attctcatgt gggatctcgg gttttccttt tgtaacgaat tttagtggat
     7981 tttttgtatt gacatacttt acaaaatata gtataatatc taaaaaatat ataaaaggag
     8041 acaatatatg gnnnnnnnnn ngtataatat tgttatcact tataattgta ttgcctaaag
     8101 cttcgccagt gagaatttta caaagagagg agtgaaaatt ctgccatgtc tgaacaatct
     8161 tctgtgcagc cgatcttcac cgaatcacag ctttgctatt tcagccggca gttttccaat
     8221 atgtttggac tgccggtacg cctctaccga cagcaggagg agatctacac ctattctccg
     8281 gtgcagctgg cagcagatcc cgtgaccctc tgcattgatg cacttttgca ggaaaccgca
     8341 ccactgggct attttgccta tcacgatatg ttttactatg gctatgtccg gcatcagtcg
     8401 tactgctttg tcgccggacc agtcagcgaa ctggcaattt cggaacacga attgaaaaag
     8461 ctgggaggtt cgctgcattt gcagccggaa cagttttctg tgtttgcagc cgagatgaaa
     8521 acgctttcgg gaatgcatcc ggacaccctt ttgcaggcaa tgatcctgta caatttcaca
     8581 gtcaaccgaa ccatgtatga catttctaat ctgcggatcc agcaaaagga acagaaaacc
     8641 attacagcag aaatgaaaga aaatgagatc ctgtccggtc cggaaaacag aaatccagaa
     8701 gggcacatgc gttctctgtc gatcgaacag gacatcattc ggaaagtgca gcagggtgac
     8761 gtagacggct tgatcgatgg tgcaacaaaa attccggcag ttcgttccgg acagcttgca
     8821 ccgcagctgc tgcggcatca caggaatttt ttcattcggc tggaaaccat tgtttcccgt
     8881 gcggcgatcc aggctggtct gcctgccgaa gaagttttag aaacggagga acactacatt
     8941 tccaaatgcg aatccctgcc ggacatggaa cgcatcaaga atttgcagta tcacatgatt
     9001 ctggactatg ccgatcaggt gcgaaagctg aaacaatgcg gcggcagcca gtcccgactg
     9061 gtgcgaaatg tgttgcagta tattcgcagc catatggcag aaccgattcg caccgcagac
     9121 atcgcagcat actgcggcaa aagccgcggc ggactgacta cggaattcaa aaagcaaaca
     9181 ggcaggacgt tgtctgattt tatccagcag caaaaaatgc aggaggcaga aacactgctg
     9241 ttaaaaacgg agcaaaatct ctcccagatc agcagtctgc tggggttctc gtcccagagt
     9301 cattttacac gagttttcaa agaagttcac ggtatgacac ccacagaatt tcgaaaatcg
     9361 caaaaataaa attagcttta cagatatata aattgcatga caatcctgca atacaagaaa
     9421 acctccttct gttataataa atgtagtttg aatcgaaact tacagc
//
LOCUS       DAWBGR010000091         9305 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2071_length_9305_cov_5.538378, whole genome shotgun sequence.
ACCESSION   DAWBGR010000091 DAWBGR010000000
VERSION     DAWBGR010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9305)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9305)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9305
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2071_length_9305_cov_5.538378"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2155 a         2571 c         2478 g         2101 t
ORIGIN      
        1 aaaaatttga cgacgcatct gtcgcacaat ctttgtgcgg tattgccttt attcttctgc
       61 cgccggcttt tcctttaccg gctccggctt cggggcaact accttaggct tccgcaggtt
      121 ttcaatacac cctctggggc attcctttgc acacaagccg caggacttac acttatcata
      181 atcgatgacc ggataggcgt tctcgacgat gatcgcctca aacttgcaga ccttctcgca
      241 cttcttacag ccgatacagg agttctgaca ggactgcatt gccaccttgc ccatattgcg
      301 ggaggaacag cgaacgtcca cctttttctt ttctgaccgc agttcgatca gcccgttggg
      361 acagactttt gcacacttgt tgcaggcaac acacttttcc gtacagaatg tggcaatgcc
      421 gtctttgatc gagatgacat cgttttcaca gacagaaacg cagtctccca gtcccagaca
      481 gccataggca cagacaccgg tgccaccgta aaaccgtttc gcagcggtac aggactgcac
      541 accgtcaaac gtgaacttcc ggttggtggc ttcgcaggta ccgttacagt gcagcaccgg
      601 caccatgcgt tccgacgcag tcacagacac gcccatgatg tcaccgatcg ctgccgcagc
      661 attggcaccg ccgggcagac aggcattcat gggagcacct ttattcacaa tggcatcggc
      721 atagtctgca cagccggcat aaccgcaggc cccgcagttt gcacccggca gtgcctcggt
      781 gatctgcacc gcacgctcat cggttttcac cgcgaacacc tttgagatca cgaccagcag
      841 aacaccgaac acaatgccca tgaccacaaa tatcagaatg ggatagacaa attccatgac
      901 acaccctcct tactgtccca gtccgccgaa gcccagaaaa ctcatggaca caatggcggc
      961 tgcgataagc gttgccggaa cgccgcgaaa catcttcggg gtttcgctct cggcatcgtc
     1021 cacgcgggaa cggacaccgg agaagatcac cattgccagt gcaaagccta cgccggagaa
     1081 cacggcattc accagcgact gtccgaagct gtagctttcg tcgatgttca taatggtaac
     1141 acccagcacg gcacagttgg tggtgatcaa aggcagatac acgcccagcg ttttatacag
     1201 cggcggcatc agctttttga taaccatttc cagcagctgt acaaatacgg caatgaccag
     1261 aatgaatgcc actgtccgca gataagtcag ttccagcggc ttcaggacaa agtagtaaat
     1321 gggataagtg cacagggacg cacacagcat gacgaaaacc accgcggcac ccatacccac
     1381 gctggaatcc agctttttcg aaacacccag aaacggacag cagccgtaaa atttcgacag
     1441 aacgaagttg ttcgtcagca cagccgcaat gatgattgca atataggtac tcatgcgtca
     1501 gcctcctcac actttgcgtg acatgccgcc gcattggggc agcttgcaca gccgatcttt
     1561 ttcgggggat tcttcctgga cagggcacag accacagcga tcacgaagcc cagcaccaga
     1621 aagccgcctg ccggcatgac aaacaggctc atggtatgtc ctaccatgcc ggggatcgtc
     1681 agtccgaacc atgtgccgct gccgaggatc tcccgcacgc tgcccatgag gaacagagcc
     1741 agtgtgaagc ccagtcccat gccggcaccg tccagagccg aagccagcac gttattcttg
     1801 gaggcgaaca tttctgcccg tcccagaatg atacagttta cagtaatcag ttccagaaat
     1861 gctcccagac tggtatacaa cgctggcaca aagccgtgca ccagaaactg aatgaccgtt
     1921 acgaacgaag caatgattac gatatagcag gggagtttca cctgctccgg aatcactttc
     1981 cgcagcagag agatcacgat attggaacag atcagcacga acatggtaga caagcccatg
     2041 ccgatgccgt taaatgccgc ggtggtaact gccagcgtcg ggcacatgcc cagcagtcct
     2101 accagcgtcg ggttttcttt caggataccc ttggtaaatt cttttccgta gttcatgcac
     2161 cggtcacctc ctgattggca tgtacagttg caagggcaca gttcacagcg gctttcatgc
     2221 ccttggaaga ataggtcgct ccggtgatat tgtccacgcc gtctactgcg tcactgtccg
     2281 acagtcccac cagatgatct accagcagtt ccggatcgga ctgcaccttt gtgcccagtc
     2341 cgggggtttc cgaaacggtg acaaaggaaa caccggtaac ggctccgtcc ttgtccacgc
     2401 cgatgacgac ctgcaggccg cctttgttat agccgtctgc ggtcaccagc acggcatact
     2461 gtccgttttc gtccagatac agaccggtgg cagctgcctt ttcgttattc tccggctgga
     2521 agtctgtgac ttcctcaaac gtgccggcat cgggcagctg tttcagactg gtctgaatgg
     2581 catctgcctc tgcggcagcg atcttgtcct tggtcaccat attggcaagt gccagaaatg
     2641 cacagcagac tgcacagatc agtgccagca ccagcggcgg cagcaccatg gattttacag
     2701 aagatgtctt tgcctctgcc atgttacttc gcctcctttt tcactgcggg ctttgcccca
     2761 aaggggtgcg tcatggtcag cttgtcgatg tacggagtca gcaggttcat cagcaggatc
     2821 gagaacgagc agccctccgg catgttggca aactcccgaa tgacaaaggt cagcaccgca
     2881 cagcccagac cgaaaatcag ttttcccgca ccggtcatgg gggtggtcac atagtcggtc
     2941 gccataaaga atgctcccag aattacaccg ccgctgagca gggcgtagag cacgtcggtc
     3001 atttcgccgc cggtggcgag ggtatagaac agttccagca ctgccagacc gcccagatat
     3061 gccacagggg tgtgccacga gatcagcccc agcaccagca ggaacacgcc gccgatgagc
     3121 agagccagta ccgaggtttc acccagcgaa ccgcccacgt tgccgaggaa caagtccagc
     3181 agaccggcat caccggaagc cagcggagtg gcaccggtca cgccgtcggg gaggtttccg
     3241 gtagcggcaa acatttcaaa gaagcttctg ccgcgataga aaaagggctt cacccaagtg
     3301 gtcatatcgc cgccgaaaga cagcagcagg aacacacgtc cggtaatggc gggattggcg
     3361 aagttgcagc ccagtccgcc gaacagctgc ttgacgactg caatggcaaa gacgcagccg
     3421 attgccgctt cccacagggg cagcgttgcc ggcagattca gagccagcag cacgccggtg
     3481 agcatggcac tgccgtcggt ataggtggtt tctttctgca tcagcttctg acagaccatt
     3541 tcggtgaaca tgctgatgac agcacagaac acaaccagaa acaatgctcg cggtccaaag
     3601 accaccgtcg ccgccacgat cgccggcacc agagccagca ccaccagcag catgatcttt
     3661 tgcgtggtca tgccgcttct cacgtgaggt gacggagaaa cagtcaataa tgatgtcatg
     3721 gtttttcctc ctattttcat ggattggtcg gagagtctgg gggattttaa atatgtgcag
     3781 ttgtaatatg aggtatcgtt cttttcagaa ggatctcaca aagcaataca tattaaatat
     3841 aacacatcga gtaaaagatt tcagtaaaac ccctccaccg cctaacggcg gtccccctcg
     3901 tactgagcgg tcccctctgc cctgcgggca tctccccacc ccgtggggag tcaccccttt
     3961 caggggaggc ttatttagtt gtcactatta taatgaatag cggggcgatg tggcttgcta
     4021 tccttgggca agtctgccgt cgccatttcc gctgggcgac gggctgggca tcgtccccta
     4081 caacaagggc tgtcaactct ccggatgcca tgcttatttt ttgatgctgg acatatagaa
     4141 tgcctttgcc agctggttgg tttctgccac cggacgcttt gccggacagg cgtaagtaca
     4201 gcagccgcag ttcatgcaca gggtgacttt cagtttgccc agtgcttcgc tgtcctcctg
     4261 ccggaacgcc ttggcgatct ccgtgggcat cagccgcagg ggacaggcgt gaacgcatct
     4321 gccgcagtga atgcaggcgg tgggtttcgg ggaaacgaat ttctccagag ccagcacagc
     4381 gttattggct ttcagaatcg ggtcggcagt gtccggcaca gcgatgccca tcatggggcc
     4441 gccgtaaagc actttctttg cccgttccat atcgcactgt gcaaaatcca gcacgtcctg
     4501 caccggtgtg cccataggca ccatgacatt gcagggcttg cctacagcat cgccgtccac
     4561 cgtcaccata cgctgcacca tcggcatgcc ggttttcagg tagcggctga gaaatgccac
     4621 ggaggacacg ttcatcacga tcacgccctg atctgccggc agctgtccct ccatgacggt
     4681 gcgtcccagt gtgtgataga tcagcacctt ttctgcaccc tgcgggtacg tagtggggag
     4741 agagaagatc tgaatctccg gttcggactt agtcttttcc gtcaggacgc ggatcgcctc
     4801 cggcttattg gtttcaatgc caatgactgc cttggggatt tgcagccagt gcagcacctg
     4861 ccgcacgccg tccagtactt cgtccggtgc ttcgatcatc tggcggtagt ccgaggtgat
     4921 atagggctca cactctgccg cgttgatgac cagcgtgtcc acggtctgtt tggggttcag
     4981 cttcacgtga gtggggaagc ttgcaccgcc catgcccacc agtccgcttt cccgcactgc
     5041 cttgaggaaa ctctctttat cggtgacagc gggcggacag acatccggag aaacagtctg
     5101 ttcgccgtcg gtttcgatct ccaccatggg acatgtcctg ccgttggaca tacggtaggt
     5161 ggaaactgcc ttgactttgc cggaaacacc ggagtggatc ggcactgcca tgaatgcatc
     5221 ggtatcgccg atcttctgcc ccaccagcac ggtatcccgc ggctttacaa gaggtgtaca
     5281 gggtgcaccg atatgcatac tcatgggaat ccgcaccagt ttcggcagcg gcagcggtac
     5341 agaagcactg ttttctgtgt tcttatggtg tgccagatgc acgccatgca gtttcttcat
     5401 agaatacagc tcctttcact ttttcggatt gctttacatt tatatatcta cagaaacgga
     5461 gtggtttctg cgataggcgt tgggaatgat ttatagggtg ggaatttttt tcttcatgtg
     5521 tgaaattcca cagaagaatc cctccaccag cttcgctggt ccccctcgta ctgagcggtc
     5581 ccctctgccc tgcgggcatc tccccacccc gtggggagtc acccttttca agggaggctt
     5641 atttctatag ttttagctgt tcatcgtatt acaatgtaca gaagttttac gcattggggg
     5701 tgaagtgcag cccgtcgctg acgtacagct tgccggactg gtcttgtgct acgatctgat
     5761 aatcctctgc gtgaaggtgt tcctccagat gctctgtgcc ctccagccag atcagcgtgg
     5821 agagaaagtc ggattgcagc ccgctgtcac agaacaccgt cactgtggtc aggtcggatt
     5881 ccgccggcat gcctgtctta gggtcgagaa tgtggcagta ttgctttccg tcatcagcaa
     5941 taaaataccg ttcgtatccg ccggaggtgg ataaaaaaca actgtcagtt gacacggtgc
     6001 ccagcgtacc gtcgccggac gggtcacgga tctcgatctg aaacggtttc ccgtcaggct
     6061 tctccccgta acacaaaatg gaagatgtca tggaaactgt gccatagccg gcggtgccgg
     6121 actggtcgag agcctctttc acgcagtcca gactgtagcc ctttcccacg gctccgcagt
     6181 ccagctgtgc tccgtcggtg agggtcacgg tgccgtctgc cgccgtcacg tggtcgggag
     6241 aaatggtttt cagtaccggt tccagttccg ccgccgtggg cacgtggggc gtatctgtgg
     6301 taatgcccca cagtgccgtc agctgtccca cggtcaggtc tacgctgtcc ccgaatcgtt
     6361 tttgcagggc agccgtttcg gacaccagct ggtatatggt atcgttccca gaaattgtgc
     6421 cgctctgatt cagccgggag atcgcactgt cctcctgata attgtccagc gtcttgtcca
     6481 gcgttttcag cagcgaagcc gtttcggaaa cgccgccgcc gtggagagaa atggtacagg
     6541 cggtatccat tgcccagacg gtttcggtct gagccggctt tgcagaagtg catgccgtca
     6601 gcaccagcgg cagcagcacc gcaaaaagtt ttttcccgtg catcataaaa acatctttct
     6661 tttccgaggg aaatgtgata taattaaagg caacaccgca caaagcccgc ctgacggctt
     6721 tgcacgtaat ctgcttgcag attacgcaca agctttgtgc ggtgttgcct aaaagccggc
     6781 atttccggac ggaacttgtc cgatttcttc aaaaatgcct accctttatt ataatacaag
     6841 ccgtttattt tgtaaacggt aaataattgt ttattttaca gcgaaattat tacctttttc
     6901 gcaaaatttc cgaagatgta tcgactatcc aacgaattcc acaatggagg tttatttttt
     6961 atgaaaggtt accgcatcag cgtgatccgc cacggcagaa ctgccgcaaa tgacaaaggc
     7021 atttacatcg gcagaaccga ttttccgctt tctgacaaag gggcggcaga gcttgccggt
     7081 aaaatggacg agttccggta tcccaacgtg gcgagggtgt acagcagccc gctgcgtcgc
     7141 tgcaccgaaa ccgcggaaat tctctttccg gacgtacaga tccagacggt ggacaatctc
     7201 attgaaatgg atttcggcac atttgaggga aaatccgctg acgaactggt acacaagccg
     7261 gagttcaagg agtggctgaa aggcggtatg gactgccgtc cgccgcaggg cgagagcgtc
     7321 aaggaagtgc agctgcgaat cttccgttcg ctgcgggaga tcattcggga catgatgcag
     7381 gaggatctgc tccactgtgc cgtcattacc cacggcggca tgatcgccaa catgctggca
     7441 gggttcgggc tgccgaaaat ttccccgcag gagttacagt gccagccggg agagggcttc
     7501 gacatctacg tcactgccga cctgtggcag cgttcacagg cgtttgagat tctgggcatg
     7561 gtgccgtatt cccgatgagg atacttttat aatagtcgga aatataatag tagaaaaccg
     7621 caccctttgc cggagttttc cggaaaagag tgcggttttt gattttgatt tgacaaactg
     7681 cggcaagttt ggggtttcat aggcaatacc gcatgaattc ttcgaatgaa agaaagtttt
     7741 cgtgtgcata tgaaaacgat ttcgcacgga gcacatatta tttcgaaaaa gcttgttttt
     7801 tttcaaaaag gcttgtattt ttctgcatat tatgatataa tgtgtttatt cctatcgaaa
     7861 cgggaatttc ggcgggattt ccgagatttc cgagatttcc gaagacaata cttccggtac
     7921 ggcaccggtt tttcaagaaa gggttataca catgacgtat gaagaagcat tgacttatct
     7981 gactgcccat ggtcagcagc acctgctccg gtattatccc actctgacac cggcacagca
     8041 ggaacacctg ctggaacaga tctccaacct gcatttggaa cgcccccagt tccagaaccg
     8101 cgaagcggca gcacagcagc ggggacactt tgcaccgctg ggggctgtga cactggaaca
     8161 gatcgcagaa aaggcggacg aataccgcag gatcggctta caggcaattc gtgatgccaa
     8221 agtgggagcc gttctgctgg ctggtgggca gggctcccgt ctgggattcg atcaccccaa
     8281 gggcatgtac aacatgggca agacacggga tctgtacatc tttgaatgcc tgatccacaa
     8341 cctgaaaaag gtcactgacg aggcggaggc gtatgtgccg ctgatgatta tgaccagcga
     8401 acagaatgat gcggaaaccc gtgcatttct gaaagaacac gactacttcg gctacagtgc
     8461 ggacaacgta cactttttca agcaggaaac ggaaccggct gtggacgaaa acggcaaaat
     8521 tttcctgaac gctcccgacc agatcgtact ttcccccaac ggaaacggcg gctggttctc
     8581 ctcgatgcag agagccggtc tgctgcaaac gatcgaggag atcgggctgg aatggctgaa
     8641 cgtctttgct gtggacaatg tgctccagca gatcgccgat ccggtattca tcggggcaac
     8701 catcgcttcc ggctgtgcct ccggcggaaa ggtcgtcagc aaggcagatc cggacgaacg
     8761 ggtgggtgtg ctctgtctgg aggacaacaa gccctcgatc gtggaatact acgaaatgac
     8821 cgaagaaatg cggacactgc ggaacgagga cggctcgctg gcatatcggt tcggcgtgat
     8881 tttgaactac ctgttccgcg tagatgctct gatgcatacg ctgggctcgg atttcccgct
     8941 gcacatcgca cacaaaaaga tctctcatct caacgaagca ggagagctgg tgaagccgga
     9001 ggaacccaat ggctacaagt tcgaaacgct ggtgctggac atggtgcaca tgcaggacag
     9061 ctgtctggcg tttgaggtag accgcagcaa ggaattcgct ccggtgaaaa atgctgtggg
     9121 cgtggactct gtggaaaccg cccgggcact gctggagcag aacggtgtcg tactgtaacc
     9181 gataccggaa atgatccgat tctgaaagag ggcaagacgg cttgtcctct tttttgtttt
     9241 ccctggggcg ataccgcaca aagattgtgc ggcagatgcg tcgtcaaatt tttcgtcaga
     9301 tgaaa
//
LOCUS       DAWBGR010000092         9298 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2076_length_9298_cov_3.729309, whole genome shotgun sequence.
ACCESSION   DAWBGR010000092 DAWBGR010000000
VERSION     DAWBGR010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2076_length_9298_cov_3.729309"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2079 a         2687 c         2293 g         2239 t
ORIGIN      
        1 aagcagatgg cgtgcaaagt cgccagacgc tctttgtgcg gtattgcctt atcttttcac
       61 gccgtatttt cgataaaacg cagcaacatc tgcctcgttc cgcaccagct ccgcatagca
      121 aagccgcttt tctgccgcat cataaaagcc gatcacagtt tcaccagtac acgtgctgga
      181 ttccattcga atatccgccg ccgtgaatct atccggaatc gcctgcgatt ttactcgctt
      241 ttgaaaaatc cgcatgcaaa cacctccata tccaaagtgc cggcgaatgg tatatccgcc
      301 ggcacttcag aaatcatcaa ttgagaacgt gtttaagacg caataaaggt acagaatgca
      361 cgataggtca tataaacatc cagcttggaa accacctcaa aaggtgcatg catggacaac
      421 accggcacac caacgtcaat ggtgtcgatg tccagatttg caatatatgc cgcaacagtt
      481 ccgccgccgc ctgcatcaac cttgcccagc tcgccggtct gccataccac atcgccttcg
      541 tccagaacac ggcgaatggt gccgacaaac tcagccgatg catcagacgt gccagacttg
      601 ccccgtgcac cggtaaactt ggtcacgcaa acgccgtggt tcagatatgc acagttcttc
      661 tgttccatta cgtccgggaa cgtgggatca aatgctgcgt tgacatcagc agacaggcac
      721 tgggaagcgt gcatcacatg gcggcccttt gctccgaagc agtctgccag atcttccaga
      781 aaatacggca gataagaaga gcacagaccg gtgttgccgt cgctgccggt ttcttccttg
      841 tccgtgagaa tcgtcactgc ggtgtgtacc ggtgtttcgc agtccagcag agcacgaagt
      901 gccggataag cacaaacgcg gtcgtcatga ccatagccgc cgatcatact gcggtcaaat
      961 cccagttctc taaccggaaa tgccggaaca gcttccagtt ctgccgacag gaaatcggtt
     1021 tcggtcatac cgtatttttc atgcagaatg ttcaggatat tcagcttgac ctgttcgctc
     1081 tcctcgtcct tgttgaacgg catagagcct accagcaggt tcagttcttc gccgcggaca
     1141 atttccgttg ccggacgctt catctgctgc ggtgccagat gcggcagcag gtcggtcaca
     1201 cagaataccg gatcaccggc atcttccccg atgtttacct ctaccgaagt accatcagcc
     1261 tttaccacaa cgccgtgcag ggacagcgga atcgtagtcc actgatactt cttgataccg
     1321 ccatagtagt gggtcttgaa cattgccagt tcgtgatcct catacagcgg attctgcttc
     1381 atatccagac gcggagagtc gatatgtgcc gcacaaagac gcacaccttc tgtaatcggt
     1441 gcagtaccga tcactgctgc aataatcgcc ttgccgcggt tgttgacata gatcttatcc
     1501 cctgccttca gctgcatatc cggtgtgaaa ggcacaaagc cctttccttc cagcagtgca
     1561 gctgcattct tggctgcttc acgttctgtc ttggacgcgt tcagaaatgc catgtagtcc
     1621 gctgcaaacg catcgctctg cttctgtgtt tccgcagaaa tgcgggtcgc accgtgctta
     1681 gacttcatga aaagctgttc ttttttctgt tttgcctgtg tttctttcga ttccatttga
     1741 aaagcctcca attcttgaac atattccgta ttccagaata tgaaacccat ttttatacaa
     1801 atgcaccgta ttccgtttcg taataacaaa tcagcttggc ggaaagttcc tgtgcagcgt
     1861 cccgcagttc ggaaaagctt gcattatttt ccagaactgt gtccgaatgc agctggaaga
     1921 attcctctgt cagctgcatt tccatacgct gcttcgccgc cgccatggag atatgatctc
     1981 gttccatgat ccgctgcatt cgcaattctg gttttgccac cacagaaatg atcaggtcgc
     2041 agaaatcgtc cgcccgactt tcaaacagtg tcggtgcgtc caataagatc agtttgtggt
     2101 tctgctggga aaaccgatgt atcattcgca gaatttcacc catgatatag ggatacataa
     2161 tggtattgag catttcgagc ttgtgatgat cggaaaaagc gatcgacgcc atcacctgcc
     2221 ggtccagttc cccgtccggc gtcaggatcc ggtcggtaaa gcactcctgc aagtctgcca
     2281 gacaatgcga gccgcgtgcg accacatgac gggaaatcgc atctgcatca atgagccgga
     2341 atccgttctg cacaaagacc ttagaaacag tgctctttcc agcaccagtc tgaccggtca
     2401 gaccgatcac catcacaccg tccagcgtgt tcatacctgt atcacctcaa atcctgctgc
     2461 acgaatcaga cgattgtaca ccgccagccc gatcccctcc ggcttcggag cacgcacgta
     2521 cactttctgt gcaccgtcgt cgtccaattc ccgcaatttt gaaaaaatct gctgtgcctg
     2581 ttccctgccg gatcttccgt aggtcagaca tggcagggag agcatggttt cctccccgtc
     2641 gaacaccagt gcataaacac cgtctgcccg attctgttcc gccagtgccg caaagttctg
     2701 ccagctgccc tccaccagaa tcacgtgagc acggggagag taatgctgat acttcatgcc
     2761 gggagaacgc acagtctgcc cttcttgcag ctggtgcaga atggcaggat ccagtgtcac
     2821 ttccggagcc acttcttcca gcatctcctt tgtgacaaag cccggacgaa gaatccgcac
     2881 tgtaccgttt ggctccagtg caatgactgt ggattcaacg cccacagaac atgctccgcc
     2941 gtccacaact gcggcaattt tgccctgcat atcccgcatg acatgctgtg ccgttgtcgg
     3001 gctgggataa ccggaaatat tggcggaagg agctgcgatc ggtacaccgg acagctggat
     3061 caatgcccgt gccaccgggt gagagggcat acgaatgccc accgtatcca gcccgccgga
     3121 agtgatctcc ggtatctctt tccgcttcgg gagaatcatg gtcagaggac ccggccagaa
     3181 ccgttctgcc agtttttccg ccagcggcgg caccgttgcc gccacctgct ccagtgcccg
     3241 catttctgca atgtgcacga tcagcggatt gtctgccggt ctgccttttg ctgcaaagat
     3301 ctgccgcact gcctccggat ttctggcatc tgccgccagt ccgtagacgg tttccgtggg
     3361 cattcccacc acggcaccgt ttcgcagcag ttctgccgct tccaaaaggt cttctgcatg
     3421 atctgaaagt aactttgttt ccaccgtgtt tacgcctctc cctgctgtcc tgccagccgt
     3481 gccagttcgt cggctgtggc aagggcatcg aatacttcgt ccagatttcc gttcataata
     3541 tcttccagcc ggtacagtgt cagcccgatg cggtgatcgg tcatgcgtcc ctgcgggaaa
     3601 ttataagtac ggattcgctc ggaacgatct cccgttccca cctgcattct ccgttccgat
     3661 gcgatcttgt cgttctgttc ctgaagcatc tgttcataca gacgggaacg gagaattttc
     3721 attgccttat ctttattttt atactggctt cgttcgtcct gacattctac gaccagacca
     3781 gtgggaatat gcgtgatgcg aactgctgat tctgttttgt tgatatgctg tccgccggca
     3841 ccgctggcac ggaacacatc gatccgcaga tctgtgggat tgatgtccac tgccacttcg
     3901 tccgcttcca ccagcaccgc aacagtgact gtggaagtgt ggattcgtcc ctgtgattcc
     3961 gtttccggca cacgctgtac ccgatgcaca ccgctttcat atttcagacg ggagtatgct
     4021 ccctcgccca gaatggaaaa gctgatttcc ttgtagccgc ccagttccgt ttcgttggca
     4081 ctaagcactt cctgtttcca gccctgtgtt tctgcataca tggtgtacat gcggtagaga
     4141 gacgcggcaa acagagaagc ctcctcacca ccggcaccgc cgcgaatctc aatgatgaca
     4201 ttgcggtcat cgtccggatc tttcggcagc agcagacgca tcagttcgcc gtgcagcgag
     4261 tccatgcgtt ccttgctctg ttccagctct gcctgtgcca tttcccgcag ttcggcatct
     4321 tcttcgccgt ccagaatttc cctggcttct gtaaaatcgt tctgtgcctg caaatactcg
     4381 tgatattttt caatgatcgg ctgcatatgc ttgtattccc gcatcagctg ggcgtactgt
     4441 tgattatccc ctaccacagc aggatccatc atcttttcct gcatcgcgtg atagttttct
     4501 tccatttgcc gaagcttttc cagcatatct gcgttcctgc ctttctttct gtcacgatat
     4561 gtaccggcat gcactccgat acgtcacgat tcgggggctt ctctccgaat ctgtttgatt
     4621 cgccgttcga tcttgtttcg cggcaccatg aattccctgc cgcagcccgt gcaacgcagc
     4681 ttgaaatcca tacccgcacg aatcacatac atggtctggc tgccgcaggg gtgcggcttt
     4741 ttcattacca gaatatccgc cggacgaacg tccattttca gccctcccct ttttgcatca
     4801 taagtacatt atattatacc ccatttctcg gaataaggca atattttctg cggatatttt
     4861 tttcgctttg ttgaaatgct ataaaatcca aacgtgaaat ccggtgaagt ttcataaaaa
     4921 gcaatttgca ttttccaatt tttgcattct tcatttcaat gctgtccgca tatttcaggc
     4981 ttctcataca tacactggaa cagcatcaga gaacggcagc actacgttgc cgaaacacga
     5041 ttctttcagg aaattgtcag gaggtcaaaa tgcaatactt accagaaggc agccggctgc
     5101 acacgccgga aaatcaagct gcactccgca gcgttgcgtc catggagcgt gccatgcacg
     5161 ccggaaccat tttagaagcc cgtgccgtcc tctgcgacag cgggcacaat ctcaccgtac
     5221 agctgccctg tatgcggggc tggatcccca gagaagaagg agccatgggc atcacagacg
     5281 gcaccaccaa ggacatcgct ctgatcgccc gtgccggaaa gcccgtttgt ttccggatcc
     5341 tccgcatcag tcaggacgaa accggtgctc ctgtggcgat cctgtcccgc cgtgccgtac
     5401 aggaagaatg ctgggagcag tacctctccc gccgccgcgt aggggatatt ctgcccgcca
     5461 cggtaacaca tctggaaaag ttcggggcgt ttgtggacat tggctgcggg atcccgtccc
     5521 tgctcccgat cgacaccatt tctgtgtccc gcatctccca cccccgcgac cggtttgtca
     5581 tcgggcagaa aatccgtgcg gtggtacggc agacagaacc gcaccgcatt cacctgacgc
     5641 atcgggaact gctgggaaca tgggaggaaa atgctgccgc tttctctgct ggtcagactg
     5701 tcgccggcat cattcgttcg gtggaaagct acggcgtttt tgtggaactg gcacccaacc
     5761 ttgccggact ggcggagccc tgcgaagatg ccgttgccgg acagtgcgcc agcgtctata
     5821 tcaaggcgat cctgccggag aaaatgaagg tgaaactgat tctgatcgac acatttgccc
     5881 agactgaccc gccgcagccg ccccgttatt tcgtgccgga aagtcagaca catctggact
     5941 actggcgata tacgccggaa tcttctcccc gcattatcga aacggatttc cgttttccca
     6001 acagcatctg aaaatcaaaa agcacgcaat tttcgttaga aactgcgtgc ttttttgtgc
     6061 ggtgttgcct ttgcttttct ccttattctt ctgattctgc aaacgctttc tcccgctgtt
     6121 ccatttgttg tgcgatcact tcctgctgca ctttggaggt ctttttgatg tggtcgccga
     6181 tgatttccga gatgtccgaa accgtaataa aggcactgcc gtcatacttt tccacgattc
     6241 gccgcagttc catgagttcc agtcgggtaa tcacgcagta catcatatct tttttgccgc
     6301 tgatgagtcc gtgtgcgtcc agaatggtca tagtacgccc cagcttttca tagggcaata
     6361 ccgcacaaag attgtgcggt attgcctata tcatatttta aagataaatg caactgcacc
     6421 aattccgtaa gctgtgcata cagtgaaatg gagcagaaac gacagccctg tacaaaaaca
     6481 ttgtacaggg tatccacaga aagaggtgcc gaattatgcc aaaagttttt ctgagccctt
     6541 ccacgcagga gtggaacccc tatattgacg gcggcaacga ggaacagtac atgaatctca
     6601 ttgccgaccg catggaaccc tatctccgtt ccagcggcat cacctatgtc cgcaacgacc
     6661 ccgaccggaa tgttgccgga gccattgcag attccaacgc cggcgatttc gacgtacacc
     6721 ttgccattca cagcaacgcc gcaccggaat ccatggcagg catgctgcgg ggcattgatg
     6781 tgtactattc cccctacgac aaatacagcg aaactctcgc ggtcatcatt gccaacaact
     6841 ttatgagcat ctatcccctg ccggacaaat gctccgcaaa acccacgacc acgctgggag
     6901 aagtgacaca gaccaaagct ccggcagtgc tgtgcgagat cggctaccac gacaatcggg
     6961 aagatgcgga ctggatccgg aacaatctga cggcaattgc agtcagcctg acccagtccc
     7021 tctgcgatta cttcggcatt ccgctgatcg aagcgggacc ggttttccgc ggcaccgttg
     7081 tcactgacgg cagcaatctg aatctgcggc agtttccctc cacacaggga gccgtgatcg
     7141 gttccattcc caacggcagc gttctcacgg tgtacagccg ttccaacggc tgggacgtgg
     7201 tttcttaccg cggcaccgtg ggttatgcca gcagcgaatt tattgtgatt tagggcaata
     7261 cccttgcaat tttttcaaat ttccggtata ataaaggaaa cactgaaatc aaggagcgat
     7321 tgccatgaaa gacggatttt tgaaaattgc ctgtgtcaca ccgccgctgc gggtggcaga
     7381 ctgtgcctat aatgccgaac aaatcattgc ccacgccaga gaagccgctg ctgccggtgc
     7441 aaaactggcg gcttttccgg aactctgcct gaccggatac acctgcggcg acctgtttct
     7501 acaggagccc ctgctccgcg gagcagagga cggactgatg caaattttac aggaaaccgc
     7561 tgacctgtcc atgctgctgg tgatagggct gccctatgtc tgcggcggtg ccctgtacaa
     7621 ctgtgccgca gtctgctatg ccggaaagat actgggcatg gtgcccaagc agaatctgcc
     7681 caactacagt gaattctacg aagtccggca cttcaccgcc ggaccggacg cagaagctgt
     7741 tccacagcag cagtccctgc accatcatac cggatacacc tttccctttg gggcaaagct
     7801 gctgttcagc tgtgcagaaa tgccggactt tacgttcggc gtggaaattt gtgaagatgt
     7861 ctgggtaggc gatacgccgt ctgttgcaat ggcaaaagct ggtgcaactc tgatcgtcaa
     7921 cctgtcgtgc agcgacgaga tcattggcaa ggaagattac cgccgcacca ttctcaaggc
     7981 gaaatccggc agcctgctgt gtgcctatgc ctacgccgat gccggcttcg gagaatccac
     8041 acaggatatg gtcttcgccg gacacgatct gattctggaa aacggttctg ttctggcgga
     8101 atccaagctg tttgcacagg gcattctcta cggagatatt gacgtgcagc ggctcgccgg
     8161 agaacgccgc cgttccaaca ccttccagtc agaacccttt ggaacaccgg ctgtgaaatt
     8221 ctccatgccg ctgacagaaa ccgtgctgca acgctatgtg gacgctgccc cctttgtccc
     8281 cagcggcacc gatgaactga cacagcgttg tgaaaagatc ctcaccttac aggcaatggg
     8341 gcttgccaca cggctgcggc atatcggctg taaaacagct gtggtggggc tttccggcgg
     8401 actggattcc acgctggctc tgatcgtcat ggtacacgcg ttccgcatct gcgggctgga
     8461 tcctgccggc atgctggctg tgacgatgcc ctgtttcggt accacagacc gcacctacca
     8521 gaatgcctgc cggctggcgg aagcctatgg ggcaaccctg cgggaagtgc cgattcagaa
     8581 cagcgtccgg cagcacttcg ccgatattgg acacgacgaa tccgtccacg atgtcacata
     8641 tgaaaattcg caggcacggg aacggacaca ggtactaatg gacatggcaa accaggctgg
     8701 cggcattgtg atcggtaccg gcgacctgtc ggagctggct ctgggctggg cgacctataa
     8761 cggcgaccac atgagcatgt acgcggtaaa tgcttccatt ccgaaaacgc tggtacgcta
     8821 tctggtagca catgaagctg cccagacaga gccggctctg agtgcggtgc tgcgggacgt
     8881 actggatacg ccggtcagtc cggaactgct gccgccggaa caggacggca ccattgccca
     8941 gaaaaccgag gagctggtag gaccatatgc tctccacgac tttttcctgt actacgtgct
     9001 gcgtctggga tttgctcccc ataagatcta ccggctggca aagctggctt ttgccggcac
     9061 atatgacaac gccaccattc tgaaatggct gaaaaaattc tactggcggt tcttctccca
     9121 gcagttcaaa cgcagttgtc tgccagacgg tcccaaggtg ggcagcgtaa cgctctcccc
     9181 cagaggagat tggcgaatgc ccagtgatgc ctgcgtgaca ttgtggcaga aagatctgga
     9241 gaatattccg gaagaataac gattaaggca ataccgcaca aagagcgtct ggcgactt
//
LOCUS       DAWBGR010000093         9240 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2091_length_9240_cov_7.060098, whole genome shotgun sequence.
ACCESSION   DAWBGR010000093 DAWBGR010000000
VERSION     DAWBGR010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9240)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2091_length_9240_cov_7.060098"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7090..7099
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2049 a         2562 c         2284 g         2335 t
ORIGIN      
        1 gaggcttatt ggcagccaac attggcaagc cccaggcgga cagcaagggc aacgagcgaa
       61 ctccgccgga cggttttgac atggctgccc cagaaaatta ctgccctcgg gcaaatgtgg
      121 gcatcgcccc ctacatattg aaaagagttt tctgtaaaac cgaaaacata ggaaaaagat
      181 ttcaccagaa gctgtccaca tgggcggctt ttgttgttgc aaaatttttt tcaaaaagtg
      241 agatattttg accctctgga ttgttatagg ggtgaagtac ttcaaaaggc aacacaaacc
      301 aaggaggtgt accacatgga cgaagatgta ttccgcggca agctgaaaca atacaaagat
      361 accgtttacc gcattgcctt tacctatctg cggaatcagg cagatgcgga agatgtcgca
      421 caggaaacat tcctcaagtt ttatctgcgg gaaaaaccgt tccccgatgt cggcagcgaa
      481 aaggcgtggc tgattcgggt gacgctgaac gcctgccaca acctccggcg gtcggtctgg
      541 tttcagaatc gggcggaact gccggaaaat ctggcgactg cgtttgaaac gccgcaggac
      601 aatgcactgt acgcggcggt gttctccctg ccggaaaaat atcgggtggt gatcctgctc
      661 tactactatg aggaatactc cgtgcaagag atctccgaaa tgctgcaacg caacccctcg
      721 accatacaga cacaactgga acgtgcccga aaaaaactga aagcactctt agaacagaaa
      781 ggtgggtttc actatggaca aacggcaata caagcagctc atggaacaaa ttcagatgtc
      841 taatgcctgc gagcagcgga ttctgaacgc agtacaccag acgaaaatgc cgcagctgaa
      901 aaagcacatc acatggcgaa aaagagccgg cattgccctt gcggcagctg cctgtgtggc
      961 agtcgtcagc gtcttttctg tagcagccgc acagaacacc agtctgctgg agaaactatt
     1021 cccgtgggca gagtcggtag acgtgccgga aacggcagtg ttcaccaacg gacagatcga
     1081 atcctttacc gtagacggca tggacggtct ggacattacc cctgttggcg tggtcaatga
     1141 tgcaaaaaat gtttatctga tgatgcgtat tgttcccagt gatcctgatt tcaaccctgc
     1201 aaaaagagcc attgtagact ataattccgg ttcaggcatt gtatatccga tgatcaacgg
     1261 ggaaaacaaa ctcgatgatg cagataaaga tgatccctat tacttgggga aatatgaatt
     1321 tttcgaacgg acagacatac cggaagaaga cggttcgtac accgcatttt atcagctgca
     1381 atttacaaac ggtattacct ttgaccatct taattttacc attggactga ctgatattac
     1441 agatctcata gaaaaacaaa atggatttgt aaaatctgaa caaacagaac tactgggaac
     1501 gatcgatgtc acagtttcac tggatacctc tgttcaagaa gtgacattga actatacaga
     1561 accgattgca attcccaata aaaacggcac tggttatctg aaacatctgg aaatacaggc
     1621 actttcgatg cagctttccg gcacaggaga tatgcagctg tttgattcct atgattccgc
     1681 ttatccagta acgatcacct atgcagacgg cagcactatg acaccagatt gtaaaaacag
     1741 cagtatatca ttcaccgacc aaagtgatac gccttgggaa tatagcattg atttcgacga
     1801 gcctatcgcc ccagaaggca ttgtttccat tgaatttggc ggcacaacta ttccattcca
     1861 ttgacagttt catcggcggc accatgcaaa gtgtggtgtt gtccgcatac tcttcctgac
     1921 accctgcttt tctccagcag ggtgtttttc tgttcgggca aaaaatcccc gaacaaacgt
     1981 cgtgacgatg tctgttcggg gagtgatttg ataataagcc ttcccttcag aggaggggga
     2041 ccagcgaagc tggtggaggg gttctctgtg gaatcttttg cataatgttt tagggcaaca
     2101 ccgcacaaag cttgtgcgta aggtgcgtta tcagattttt cgacagattg cccagaaatt
     2161 ccgcaggcat actgccgtat ggcaagggat ttctgggcaa tatgacggaa aatctgcacg
     2221 cagattgcgt gcaaagccgt caggcgggct ttgtgcggtg ttgccttagg ttacacggaa
     2281 gtacccctca gtcacctacg gtgacagctc agcacaaggc acgccctacg ggtgccctaa
     2341 aggggagcct gaataagagc cttcccttta gggaagggga accagcgagg ctggtggagg
     2401 ggttctctgt ggaatctttt tctgaataca tcaggttcta cagaaaaact gacaaaactg
     2461 atattagccc tttctccgct tgcggttata gccgccgctg cgggcacgct ggttatagcc
     2521 gtgacgggac tggcggtgct gtccgccgtt tctgccgccg cgtccgcgag agtcgccgcg
     2581 atagtcgtta tctcgttcct cataccgccg cacggcaacg gacttgccgt tgatcttttc
     2641 gttctgcatg gagtcgatga cgaaatcggt ttccgtttcc ggcacttcca cggtggtgta
     2701 ctggtcgtga atgtcgatct tgccgaactc gttgccagcc atgccggtgg cttctaccag
     2761 tgctcccaga atgaaattcg gtgccatgcg gtcttttctg ccggcgttga tgtgcacccg
     2821 aaccgtcttg gctccggcac gcttgccctt gcgaataggc ttcggcacgt caaatgccgg
     2881 caggttcttg gtggcatcgt ccagctgctg ctgaatcaga gccgccagca gctgttccgg
     2941 tgattcggtc tgtgccagca gttcctgtgc cactgtcagt gcttcttccg aagccggtgc
     3001 tgccgccagt tccagcagct tggcacgggt ttccgctata gctgcactgc ggagttcctt
     3061 ggaagtgggc accggaatct cggtgatatc cgccttggta tagcgggaga tttcccgcat
     3121 ctccatttcc tgctttctgc cgctgagaat gttatacgcc gcaccggtct ttccggcacg
     3181 tccggttctg ccgatccggt ggatatagta ctcatgatcc tgcggcaggt cgtagttgaa
     3241 taccgcgtcc acgcccttga catcaatgcc gcgggctgcc acgtcggtgg cgatcagaat
     3301 ggtacttctg ccgcttttga agccgttcat cacctgtgtc cgctgaagct gcttcatgtc
     3361 gccgtgaagc cctgccgcag aaaagccacg cagacagagg gcttcggtca gttcgtctac
     3421 cattttcttg gtgttgcaga agatcatggc agacttaggc tgtttcgaca gcagcaacag
     3481 ctggagagca tcggttttac gtcccatggg cacgctgaca gaaaactgtg caatcgcatc
     3541 tactgtccgg tgggcactct gaatctgcac cagcaccggc tctttttgat actgcttggt
     3601 aattgccaga attgccggcg gcatggtggc ggagaacagc acagtctggc gttcctccgg
     3661 aatctgtgcc agcaccgatt cgatgtcctc gcggaagccc atgttcagca tttcatcggc
     3721 ttcgtccagc acgatcgttt tcaggttgtc cagcttcagg gtacggcggc ggaggtggtc
     3781 aaggacacgt ccgggagtgc cgaccaccag attggcaccg tttttcagct ggtagatctg
     3841 gcgttccatg ctggcaccgc cgtagactgc caccggcttc acctgcttct gaaagcgggc
     3901 aaacttacgg atctcgtcgc atgcctgcat tgccagttcc cgtgtgggac agaggatcag
     3961 ggcacagaca ctgcggcgtt cctcctgcgg cagctgtccc atttgttcca gaatcgggat
     4021 actgaacgcc gcggtttttc cggtaccggt attagacttg ccgatcacgt ccctgccctc
     4081 cagcagcagc gggatcgcct tttcctgaat ttcagtcatt tcggtaaaat ccagctgtgc
     4141 cacagcttcc agcagcggtg tggatagatt cagttcttca aatcgcattc tttttccttt
     4201 ccgattttgg gcaataccgc acaaagattg tgcggcagat gcgtcgtcaa atttttcgtc
     4261 agatgaaaca aaaagcggag tgaatacttg atgtattcac gagcattttt gtgaaatatg
     4321 acgggaaatt tgcaagcaga tggcgtgcaa agtcgccaga cgctctttgt gcggtattgc
     4381 ctttgctgtt cctgctggta ctgtcagggc attgctcccc tgcctgtgct ctgttgccgc
     4441 atctgcgaac tcacaataac cattcaactt tcccatacga tgtctgtcat accatgaaca
     4501 gcagttttct ctctttttcg tttccgcaaa aaagcgtacc cagcaccgcc cgaggcgaaa
     4561 ccggatacgc caattttctg ttaaaatatc cgccgaactt tgtgcggcgt tgccttagtc
     4621 ttcctccggc atgctccagt tgtggaatac gttctgcacg tcgtcatctt cttccagatt
     4681 ctccagcagc aggttcatct tcttgactgc ttcttcgtcg tccagtgtgg tataggtaga
     4741 cgggatctgc atgacttctg cggacagcac gttatagccc ttttcggtca gaccttcccg
     4801 cagaccacgc agagccgccg gtgcacagga aacctcgatc acttcttcat cgtccatgga
     4861 gaagtcgtct gcatcaaact cgaagcagtc ctccatgacc tgatcttcgt ccagaccgtt
     4921 gttttccagc accagcacgc ctttttcgct gaacataaag gatacgcagc cggaagtgcc
     4981 cagattgccg ccgaacttat cgaaatagtg gcggacgttt ccggcagtac ggttcttgtt
     5041 atcggtcagg cattccacaa tgactgccac gccgcagggg ccatagccct cgtatacgat
     5101 agattcgtag ttgttcttgt cgccctcgcc ggctgccttc ttgatgacac ggtcgatgtt
     5161 gtcgttgggc acattgttgg actttgcctt tgcgatcaga tcccgcagct tggcattgct
     5221 gctgggatcg gggccgccct ctttcacgca aacggtgatc tctctgccga tcttggtgaa
     5281 aattttgccc ttgattgcgt cagttgcttc ttttttccgc ttgatattat tccacttcga
     5341 atgtcctgac attggattca cactccttat tcttccgcag gcgtttcgcc tgtatgcatc
     5401 acaatttcaa acagttcttc cagccgttct ccggcaccgg tcaggtgcag atagccgaac
     5461 aggctctcca gtccggtcgc cctatgataa tcccgtgcag aagtatggcg gggaacagtg
     5521 ttattggcat ttctgccgcg tttatagaca ccggcttcgt cctccgtcag gtggggcaac
     5581 agtcgctctg ccgccgccgc ctgaaacgca gcacacacca gctgtacctt ttggtcgtgg
     5641 agttttgccg ccggctgatt cgcctgccgc agcaggtatt cccgcaccat gacctcgtac
     5701 acgctgtccc ccagaaatgc cagtgccatg gggctgtatt gtcttgcctg tgcctgtgtc
     5761 aacggttcca tctgctcacc tcattacggc acaaagtcga atgcaaagcc cagaatgtct
     5821 acggtgtcgc cctcctgaat gccttgttct tccagtgcat cgataatgcc actgctgcgg
     5881 agcacccgct ggaagtattg cagagaggaa tagtcctcca tgttgactgt ccgcaggatc
     5941 ggttccagcc acggggcgtt gacatagtac acgccgtctt ccacttcgat ctcgaatttg
     6001 ccatgggcat tttctgccag ttcttccggc gagggagcct ctgcctcgta ttgcaggatc
     6061 ggcggcaggg tttgtagcag ggctgctgtc gcctgtacca gttccgcagt gccctgtgtg
     6121 gtcgctgcgg agatctcgta gaacggcagt cccttttcct cgatatactg ccggaaccgt
     6181 gcgatctgtt ccggctctgc catgtcgcac ttattggcaa ccacgatctg cggacgctct
     6241 gccagatctt cgcggaaccg ttccagttcg ccctgaatgg tgtcgtaatc tgcaatgggg
     6301 tcacgtccct cactgccgga aacatccacc acgtggagaa tcaggcggca gcgttccaca
     6361 tgccgcagga agtcgtgtcc cagaccgaca ccgtcgctgg caccctcgat cagtcccgga
     6421 atgtctgcca tgacaaagga ctgttcctct gccacgcgaa ccatacccag caccggtgtc
     6481 agggtggtga agtgatagtt ggcgatcttg ggctttgccg cactgactac ggagatcagg
     6541 gtagacttgc ccacattggg gaagcccacc agtcccacat ctgccagcag cttcagttcc
     6601 agtgtgacat caaatgcctc acccggaaag cccggctttg caaaacgggg gatctgtcgg
     6661 gtagatgtgg caaaatggat attgcccttt ccgcctctgc cgccctttgc aaggatctgc
     6721 ggctcatcgg tggagaggtc tgccatgatc cgaccggtct tggtttcccg caccacggtg
     6781 ccgcggggca cgcggatcac cagatccggt gcactctttc cggtacaatt gcgggcaccg
     6841 ccgttttcgc cgcgttgtgc cacatacttc cgcttatagc ggaaatcgat caaggtagaa
     6901 aagttgtcat ctacctgaaa gaccacgtcg ccgccttttc cgccatcgcc gccgtccggt
     6961 ccgcctgccg ccacgtactt ttcccgatgg aaagaaactg caccatcgcc gccgtcgcct
     7021 gctttaatag aaatccgtgc ctgatcgaca aacatctctc acatctcctc tcatatcacg
     7081 gtgcatttcn nnnnnnnnnt aataagaaaa tcccaagcat acaaacgctg ctcgggattt
     7141 ccagtcgttc ttcactgatc cgacaaaatt gtcgtttcgt tactgtgcag cgtatacgct
     7201 gaccttcttg cggtcacggc caacacgctc aaactttaca gtaccatcca cggttgcgaa
     7261 cagggtgtca tcagaaccga tgccaacacc tgcacccgga tgaatgtgag tacctctctg
     7321 gcgaaccaga atgttgccag cctttacgaa ctgaccatct gcacgcttta cgcccagacg
     7381 cttggactca gagtcacgac cgttcttggt agaaccggaa cccttcttat gtgcaaaaaa
     7441 ctgtacatta atcttcagca ttttcttaca cctccatgtg tgtgacttgg atattcgcag
     7501 gaaagacctt ggacaactcc cccagctgca attccagacc ggcaaacagc cgctgaacat
     7561 ttccggacgg atccggttct ttcagctgta tcaaaaccaa tgccggttct tcctgttccg
     7621 ccgccgaaac ggggagccgg aaaatttccg tcatcaggtt tgccacagtc tgtacggtcg
     7681 cggaaacata ggcacagatc acatctgtcc cctcctccga ataatcggcg tgtccgctga
     7741 tctcacagcc ggtgtactgc tctcctgcaa gaaaaaaacg tgcggaaatc atttatgcct
     7801 cgatggcttc gatctgcacc tgtgtatacg gctgtctgtg accctgcttc ttcttttcgc
     7861 ccttcttcgg cttgtaggtc ataaccgtaa tcttctttgc cttgccattc ttgatgacct
     7921 tggcacgtac agaagcaccg tcaacatacg gagcaccgat cttcagaccg tcctcgccgc
     7981 caactgctac aaccttgtca aaaacaatgc tgtcttctgc ttccacgttc agcttttcga
     8041 taaagagcgt gtcgtttgcc tttacctgat actgctttcc gccggtttca attactgcgt
     8101 acataattct tggcacctgc cttttacaaa aactcgctgc tgaaggcaca ccaaagtgtt
     8161 tcgaaacctc cacgcatgcg gcttttttat tataccatac ttttcggggc ttgtcaacta
     8221 tttttttgaa aaaaatctgc cggcaggact ttccccgccg gcagcgtttt ctggcgttcc
     8281 agaggtgatt cgaacacccg acctaccgct taggaggcgg tcgctctatc cggctgagct
     8341 actggaacat gacagaaacg gtgcacggtt cgttggaacg tgtccgtttc cgatcaaaaa
     8401 aaagtttatg cctttacgcc gtactgttcc cgatatgcca gcattgcgtc cagatgttcc
     8461 ttgcccggaa tgacctcatc gcggattccc tgaatgatgt cctccatgtt gacgatagaa
     8521 tataccggaa cgccgtactt ttcatagaca gcctgtactg cggacttttc gccctccagt
     8581 gcacgctcca tacggtctac agtgatgacc atgccggtga tgtccacatc agcagcactt
     8641 ttcagcttcg gatacacttc agccattgcc ttgccggaag tcataacgtc ctcgatgata
     8701 acgacctttt cgccgtctgt cagcttcttg cccacaaaca tgccaccctc gccgtggtct
     8761 ttgacctcct tgcggtcgaa gcagaagttg acatctttct ggtacttgtc cgccagagcc
     8821 actgccgcag ctgctgccag cggaatgccc ttataggcag gaccgaacac ggtttccacc
     8881 ggaatgttgt gctcggcaat gcaggctgca taatagcgtc ccagttccat cagctgggca
     8941 ccgctgtgat agttgccggc gttgatgaaa tacggtgctt ttctgccgct tttcagggtg
     9001 aattccccga acgtcagcac gccgcagtct accataaaac gcaggaatga ttctttgtat
     9061 gtcatcagtt ggttcttcct ttctgttaat ccatgcacac agcgttgccg ctgtccatac
     9121 tattattata gcgaaaatca gaagccttgt caaggaaatt tgctggggaa tcaattttca
     9181 taatagcctc ccctaaaaag agaagggaac ccccctcagt cagtgctacg cactgccagc
//
LOCUS       DAWBGR010000094         9092 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2125_length_9092_cov_3.605400, whole genome shotgun sequence.
ACCESSION   DAWBGR010000094 DAWBGR010000000
VERSION     DAWBGR010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9092)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9092)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9092
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2125_length_9092_cov_3.605400"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5961..5970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7795..7804
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2739 a         1761 c         2248 g         2324 t
ORIGIN      
        1 cggggtaaat ccctttggaa ctattttatc gcttttttag tgccgaatca acgtttgggt
       61 gtggttggcg gttacttttc atccagttct atggtagagt agatgcagtt catttgttcg
      121 caaaccatca aagcagcacc gctgcactga ccttcgaaag atgatcggta taaggtgtac
      181 ttgtggaggc ctatgaaaaa ttgcttgatt tttcaaaaaa atatagttat atcaaatttg
      241 aaaaactaaa gtgatataat atcaatatca aaagaaataa aacctatttt tataggagga
      301 aattcaaatg gctcaattag gattgtttcg ggcaattgct cgaatttaca ccaatacacc
      361 agaagcacag gcaattttgc agcagctgcg gaaactgttt caagatgctg aggagaagaa
      421 tgcggtttca caggaagatg ttgtgtggag tgaattttct gaggatgtgc aaccggacta
      481 cacagagtat catctgaagt cgggatacag ctgtctgtgc agatggctga cttttcagat
      541 cagtggcatt ttaagaaaaa tcgaagcact tcacaatggc tcttacgccc tgatgtccgg
      601 cagtgctgat cttagcagtt tctgctggtc gtgtggtgca gtaagctctc aaaagcttcc
      661 gctttcggaa tacaacggaa aaacagaatg gggtgtcgat gatgctttta cggcagaaat
      721 caccatgaat ctgaaaggct ggatggagct ttttcaaacg aaaaaggaaa acgtgcttgc
      781 acatatgata aaatattaca gctgttcatg ggtgtttgat gaaaaaacaa tcaaaatcct
      841 caaaggcaaa aatccggaca gttttatcga agaactgtac tatatgagag gtgaagaaga
      901 gtaccttata ttcgattcag gtgtgttttt gggctatcgt gagccggatc cgcaaaaacg
      961 ctatgttcct ttcgaacagt ttgagtgtca tgaagaggat cggattcgta tagaagttcg
     1021 tgcatctatg tataatttca caaaagatac gattactttt gcgcgggaag atttttatga
     1081 ttctctgaaa ttcctgcagc agttcgacaa agacgcattg ctccagatgc actttgtaaa
     1141 tgttatgtac gatgattttg acaatgcgat ctatagaaac gaaatgaccg gagattttac
     1201 gacattacgc tttcggaccg gtgcgaattg gtttgaagaa ccggtggtag aagttccgct
     1261 gattggaaca gaaccaactg acaatgtatg accgaaatgc aattcacggt atataatcaa
     1321 cagcattttt cagcaaattt caaaataaat cttgatttta gtcataagaa agtgtataat
     1381 agtggtattg aatttgtaat gataaaaata ttttaggagg gcgtttccaa aatgagcaat
     1441 aacagtgatt ttcagattga agatggtgta ctgatagagt atactggtaa ggataaggat
     1501 gttgtgattc cggacagtgt gaccagtatt ggagaaaatg cgttttattg gtgtggaagt
     1561 ctgaccagca tcatcattcc ggacagcatg accagtattg gagattcagc gttttatgag
     1621 tgtaaaagtc tgaccagcat caccattccg gacagcgtga ccagtattgg aagatatgcg
     1681 ttttatggct gtacaagttt gaccagcatt acgattccgg acagcgtgac cagtattgga
     1741 tacgaagcgt tttgtggctg taaaagcctg accagcatca cgattccgaa cagcgtgatc
     1801 agtattggat ataacgcgtt ttgtggctgt aaaagcctga ccagcattat aataacagat
     1861 ttaaaagcat ggttagaaat tgatttcggc aattattgga gtgattcacg ctataatttt
     1921 tttttgagaa acaataaaat cacaaagctt acgattcccg atgatgtgac cagtattgga
     1981 aatggtgcat tttctgactg taaaagtctg accagcatca cgattccgga cagcgtgacc
     2041 agtattggta aagatgcgtt tggtggctgc gaaagtttga ccagcatcac gattccggac
     2101 agcgtgacca gtattggtga agaggcgttt tgtgtctgcg aaagtttgac cagtatcacc
     2161 attccaaaca gcgtgactag tattggagaa tttgcgtttt ctggctgtac aagcctgacc
     2221 agcatcacga ttccgaacag tgtgaccagt attggtgaaa atgtatttga agactgtaca
     2281 agtctgacca gcatcacgat tccggacagc gtgattagta ttggatatcg tgcgttttat
     2341 gagtgtgaaa atctgaccag catcacgatt ccggacagtg taaccagtat tggagattgt
     2401 gcattttctg actgtacaag cctgactagc atcacgattc cggacagcgt gactagtgtt
     2461 ggagattgtg cattttctga ctgtacaagc ctgaccagca tcacgattcc ggacagcgtg
     2521 atcagtattg gagagagggc atttgaagac tgtacaagcc taaccagcat taaaattgct
     2581 gcttctaatg aaaagtatct tattatcaac aactatgata aagtagaaag agatattgtt
     2641 gcagcaactg aagtattccg aactagaaaa ctggattcag aagtaaagat accgctggat
     2701 ctgaaaattc agctggctgt aaaactgatt gactggtatg acaatgcaga agccaaggcg
     2761 tatgtgaaaa aaatgctcac caggggtatg aaagcattga tcgacagcgg caatctggag
     2821 ctgattcagg tacttctgga aaagaccgac tttgtcacca agagaaacgt ggacaagtat
     2881 atccagtacg ccatcgacac caagcagcag gagatctatc tgaccttgat ccactataag
     2941 gacgagatcg gggcgtatac ggagatttcc aagaagttcc ggctgtgatt tcggtgacac
     3001 aggaaaacag attacaaaaa gacaggatgt gaagatatga acgaatccaa tgaagaattg
     3061 gctttggacg atgtgctgaa aaaaatgcag cgagccgatc agctttcccg acaggtaatc
     3121 agctatgcca gaaatacgct ttttgtgcat ctgcggttta tggaaaacgc actgagccgt
     3181 ctggtgtttg taccctatgg cggaacgatt gcaacggata tgtgccgcct gtattataac
     3241 agtcagttta ttctgccgga ctttcagaaa aatgacaaaa cgctgacgca ccgctatctc
     3301 cacatcatac tgcactgcat tttccggcac gcctatgttg gccctgcgat tcgccgaccg
     3361 gtctggaatc tggcgtgcga cattgccgtg gagcagatga ttcaggaatt gcatctgaag
     3421 tgtattgatg acgactacgg gaacgcccag cgtggcatac tgcaggaact acaatcccaa
     3481 ttgaagtaca tgacagcgga aaaaatctat cattattaca taacccaaaa tatttccgat
     3541 gcacagtgtg aacaactgca ggaagcgttt gcactggatg aacacgaggt ctggtatgct
     3601 cctggaaacg acagtttcgg ctcacagggc aacggcgatt ccgattcaca gggtgacagc
     3661 aatcaaaagc cggattccga cagctcgcag tcgcagaaca acgaaaacag taccgctgtt
     3721 atgactctcg ccgaggcaca ggaggtctgg aaaaagcttg caaatcagat acaaacagat
     3781 ctggagacgt tccagaaaca gcatggaaca gacggcggca gcctgctgca aaatctgaag
     3841 gcagtcaacc gggaacgcta tgattatgcc gagttcctgc gaaaattcgc tgtgtatggc
     3901 gagatcatga agattgacga tgacgagttc gactataact tttacaccta cggcatgaat
     3961 ctgtacggga atatgccgct gatcgagccg ctggaataca aagaggtgaa gcgggtacgg
     4021 gactttgtga tcgcaattga cacttccggc tcggtttccg gcgaaacggt gcagaaattc
     4081 gtcaacaaaa cgtacaacat tctcaagcag caggacagct tttttacaaa ggtgaatatc
     4141 tatatcatac agtgcgatgc ggagattcag gaagtgcaga aaatcaccag tcaggaggag
     4201 tttgaggcgt atatgaaaac catgcagctg cacggctttg gcggaacgga cttccgtccg
     4261 gtgtttgcct atgtagacga actgatccgg cagcatgcat tcaccaactt aaaaggaatg
     4321 ctttatttta cagatggttt cggggatttt ccggagcatc agccggatta tcagattgcc
     4381 tttgtatttc tggatgatga ttacaataac tatcaggtgc cggtctgggc gattcggctg
     4441 atcttgacca cagaagaaat tgaacagtag gattggagga gaaagcaatg tattttcaaa
     4501 ttgaaaatgg tgtgctgaaa aagtatactg ggaaggataa ggatgttgtg attccagacg
     4561 gtgtgaccag gattggaaat ggtgcgtttg aagactgtaa aagcctgacc agtgttacca
     4621 ttccggatgg tgtgaccaag attgatgaat atgcgtttcg tggctgtgaa aacctgacca
     4681 gcatcaccat tccggacagc gtgacctata ttggtgaaga tgcgttttat ggctgtacaa
     4741 gcctgactag catcaccatt ccggacagcg tgactagtat tggagaatat gcatttatgt
     4801 actgtaaaag tctgaccaat atcatgattc cggacagcgt gacctatatt ggtagagatg
     4861 cgttttatgg ctgtacaagc ctgactagca tcaccattcc gaacagagta accaaaattg
     4921 gaaatggtac gttttctggc tgtacaggcc tgaccagcat taccattccg aacagtgtga
     4981 ccagtattgg tggaggtgcg ttttttggct gtacaagcct ggtcagcatc acgattccag
     5041 aaagcatgac cagtattggt cacaatgcat tctataactg tacaagtctg atcagtatca
     5101 tgattccgga cagcgtgacc agtattggtg aatgtgcgtt tgaaaactgt acaagcctga
     5161 tcaacatcac aattccaggc agcgtgacca gtattggatg cagtgcgttt gaaaactgta
     5221 caagcctgac cagcatcaca atcccggacg gtgcgaccag tattgaagga cgttcatttt
     5281 atggctgtac aagtttgacc aacattacaa ttccagacag cgtaactagc attgaatatg
     5341 gggcatttgc tgactgtaca agtctgacca acatcaccat tccgaacagt gtgactagta
     5401 ttggtggagg tgcgtttgga gactgtacaa gcctgactag cattatcatt ccaaacagcg
     5461 tgaccagcat tgcaggcggt gcattttctg actgtacaag cctgaccagt atcatgattc
     5521 caaacagcgt gaccagtatt ggagattgtg cgtttatgag atgtacaagc ctgaccagca
     5581 tcaccattcc agacggcgtg accagtattg gaggcggtgc gtttaggaaa tgtagaagtc
     5641 tgactagcat caccatctcg gacagcgtga ccagtattgg aggcgatgca ctttatggcg
     5701 gtgcgtttga aaactgtaca agcctgacca gtatcaccat tccaaattgt gtaaccaata
     5761 ttgggaaacg tgcgttttac ggctgtacaa gtttgactga tatcacaatc ccaaaacgtg
     5821 taacgaatat tggaaaagat gcgttttatg gctgtcaaaa tattaaaatt gccgtttccg
     5881 atgaaaagta ttttgtggtt gatggctacg aggaatctgg aaaagatatt gttgcagcga
     5941 aaagaatact tctgtctaaa nnnnnnnnnn gccaagacgt atgtgaaaag gatgctcacc
     6001 aagggcatga aagtattgat tgacagcggc aatctggagc tgattcaggt acttctggaa
     6061 aagacggatt taatcaccaa gaaaaacgta gacaagttta tccagtatgc cattgacacc
     6121 aaacagcagg aaatctatct gaccttgatt caccataaag acgagatcgg ggcgtatacg
     6181 gagatttcca ggaagttccg gctgtaatag agcagaaagg gaagtataac catgaatatt
     6241 catgaagcaa aagagcaaat caaaaacgca gtgactgctt atctgacaaa ggatgagctg
     6301 ggacgctatg tgattccgcc gcagaaacag cgtccgattt ttctcatggg gcctcccggt
     6361 gtgggaaaga ctgccattat ggaacaggtc gcctcggaat tgggcatcgg tctgctgtcc
     6421 tattccatga cgcatcacac cagacagtcc gcattgggat tgccgctcat caagcacgtg
     6481 aactatcagg gcagcgagta tgatgtttcc gaatatacca tgagtgagat catctcttcc
     6541 gtctatgaac tgatggaaaa caccggcatt caaaacggca ttctgtttct cgacgagatc
     6601 aactgcgttt cggagacact gactccgatc atgctgcaat ttctacaata taaagtgttc
     6661 ggcagacatg aagttccggc tggctggatc gttgtcactg ccggaaatcc gccggaatat
     6721 aaccgctctg tccgggagtt tgacattgcc acatgggaca gactgaaacg ggtagatgtg
     6781 gaaccggatt ttgcggtgtg gaaggagttt gcatatctga atggggtgca ctcctcgatt
     6841 ctgacctatc tggaaatcaa aaaggggaat ttctataagg tggaaactac ggtggacggc
     6901 aagcgttttg tgaccgccag aggctgggac gacctgagcc agatgatcca cctgtatgaa
     6961 caacacaaga ttcccgtcga tcaaaaactg gtggaacagt acattcagaa tccggaaatt
     7021 gcacaggatt tcgccattta ctatgacttg tacaacaagt acaagagcga ctatcagatc
     7081 aaagacattc tggcaggtac agcaagcacc gatattcagg aacgtgcaaa acgagcaaag
     7141 tttgatgagc gtttctctct ggtgggactg ctgctggatg ctgtgatggg tgccatgcgg
     7201 gagtctacgg aagaagagga cgtattggga gaactggtca agacgctgcg tgtgttcaaa
     7261 cgggaatcca agactgagga cggtgcagca ctgctgaaaa aatatatcca gaacaaccga
     7321 aaaaaactga atgccggcat tcgggcaaaa agtctttctg aggcacagca gaaacaaaag
     7381 ctgcaggtaa tgcggattct ggaggaacag agcaaactgg tgcagtctga tccgaagcag
     7441 gcagcttttg cactggtgaa aaaggatttc gataatcggt taaaggcatt ccaaaagcgg
     7501 agcgacgcat tgtcggagca ggtgcggcat atgtttgcgt tttgtgacac ggtctttccc
     7561 aatggacagg agcagttgat tctggtgaca gaactgacgg cgaatccgta tagtgcaaga
     7621 tttttgggtg aacacaaggt cgatgcgtat tttgtccacg ataaggaatt gatgttccat
     7681 cagagacagc tggaactgaa ccaggaaatt gcggatctgg agctttaaga aaactctttt
     7741 tttgcaactg ccaaattgaa gtgcgatgag gctcagtgca gcgaaaagtg cgtcnnnnnn
     7801 nnnntgcgtc agtttccagt tgtgttatat tgaaaatata ggagaaaaac aaaatgagca
     7861 gctgccgtga ttttcaaatt gaaaacggta tattgacaag gtacactggc gcaggcggag
     7921 atgttgtgat tccggacgat gtgctcgtta ttggagatga ggcgttttca gattgtgtaa
     7981 atctgactgg cattaaaatt ccggacagag taattcacat tggagaaatg tcatttcaaa
     8041 attgtatcaa cctaactaga atcacaattc cggatagagt aacctgcatt ggagatcatg
     8101 cgtttagagg atgcagaaaa ttgataagca tcaacattcc aagaagagtt gtccgtattg
     8161 gaatgggcat gtttggcggt tgtacaaact tgacgaatat cacactccca gaccgggtaa
     8221 cctatattgg agacagcgtg ttttggggat gcaaaagcct gaccagcatt acaattccag
     8281 atagagcgaa cagcattgga gatgaggcat ttgggatctg cgaaagtctg accaacattg
     8341 caatcccaga cagtgtaacc agcattgggg caggagcatt tttgcattgt acaagcctga
     8401 ccagcattgt cattccaaac agtgtgacca gtattggaaa aagtgcgttt tctggctgtg
     8461 cgagcctgac cgatattacc attccgaaaa gcgtgagaag tattggacaa aaggcctttt
     8521 atggctgcaa aagcattaaa cttgcggctc ctaatggaag agcttttatg attcgctgtt
     8581 atgataacat ggaaaaagat attgttgcag caatgcaaat gctaagaatg aaaaaaatag
     8641 atacaagagc gaagatgtca ccaagtctga aatttgcatt ggccttaatg cttttcgact
     8701 tttatgatga tacagatgcc agagcatatc tgaaaaggaa tttagcaaaa ggcatgaaag
     8761 cgctgatcga tgatggtaac ctggaactgg ttgaaatact tttgaaaaag acagatctcg
     8821 ttaccaagtg caacatcgac aagtgcatcc aatatgccat cgacaccagg cagcaggaga
     8881 tctatctgac cttgattcac tataaaaatg caatcggagc gtatgcggaa atttctgaaa
     8941 aattctggct gtgagaatga tgggacagga gaaaagcgaa ttttttaatg atagcttgtc
     9001 aagtcaagcg caacacaatc agatattcag aggattcgac ccaccgaaaa aaagtctgta
     9061 aaaacgcagt caactatggt aaaatataaa ta
//
LOCUS       DAWBGR010000095         9091 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2126_length_9091_cov_4.661908, whole genome shotgun sequence.
ACCESSION   DAWBGR010000095 DAWBGR010000000
VERSION     DAWBGR010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9091)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9091
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2126_length_9091_cov_4.661908"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3912..3921
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2210 a         2453 c         2207 g         2211 t
ORIGIN      
        1 acatcatctg ccagacgctt gccctcggtt tcccgcattg ccacgaactg ctgcaacgct
       61 tctgccatga ccggctggac ttcgctccag atcacatcgg gatcatctgc cacctgctgc
      121 acctgaaagg catcgggaat ccgcagcagt gtggaaagcg gcagggcatc tgtcgcattg
      181 ccgttttcgt ccgccgcagt gcccagcatg atctggttcc tgcggaagcc ggactcccga
      241 agcagtgcat tttccgccgc aacggcttgc agatatgccg tcaccacatc gtgattgatt
      301 tccaccacag tggattttgc accgatcgtc tgcatggtga gcgttacctc aaccttgccg
      361 cgggaaattt tctgctgcac ctgttctttc agtttttctt ccagatagct gaagctgcgg
      421 ggcagccgca cagaagtttc cagaaaccgg tggttcacgg aatgcacctc tgcggtgatc
      481 tcatagcgtt ccgtccgtgc tgtcgcacga ccgtatcccg tcatactttt caaaaatgtc
      541 acactcctcc ggaaaccgcc agagtttcca tgcaataata ttccatagta tcttattata
      601 gcattttggc acagaaaaat caagtcatat tgtgaaactt tttccgtgcc acccagacag
      661 cagacaccga caatacaaaa aggcactaca gcctttacag ctatagtgcc ttttgtaata
      721 gatgaaaatc gttgagattc gtgattctct tatgcgttca tgctggagtt ttccggcaga
      781 cccttcagaa tgtgaaccag gaacttcagc agagcagttg catcgttgcc gtcgatttcg
      841 ccgttctgat cgtagcagtc gccgttcaga gtctgctgtt cgttgaagga tacaacattt
      901 gcagccttct tgttcagcag aactgcgtct gtaacgtcta cagagccgtc cagattggtg
      961 tcgcccagca gcagctgacc cggagtgata gatacagaag aaacagaagt agatgcagta
     1021 gttacatcag aatctgtagt ttcagtagtt acatcagtgg tagcagttgt atcagtagta
     1081 gtagcagcct taacagttac ctttacttca gcagtcttgc cgtccttgga aaccttgatt
     1141 gtggtttcgc cttcggaaac accagttacg ttaccatcag catctacaga agcaatggta
     1201 gaatcagcag actcgaaagt gcagccagtc tgattcggct tcagggtttc gctttcgcca
     1261 acagtgatct caatatcaga cggtgttaca accagatctt cagacggagt atcgtcacca
     1321 tcaaccggca tatccggata cagttcatgc atagtaccca gagccatcat gatgtcacct
     1381 gcatgccaga atctgtgcag agtgaagtag tagtcatcgg tgttgccctt tgcatcatag
     1441 cattccttag cataatcgta catggtcttg ccgttagcgt ccttgctcat gtactcgcca
     1501 gtggtatcct tatagttgga acgaatgtct acgaatgcta cgccgtcctt aacggtatca
     1561 ccgttcggca ttgtaccggt gtaacggcta gcggacagaa ccttgttttc accgtttgca
     1621 cgagtgccgt ccggcagagc cagcttggtt tcccacagtc tcttcaggtt ggtgttgtgg
     1681 tcggaaacgc ccagaccaat atcatcacga tacttgttcc actctctgtc gaggagctgt
     1741 ttgccgaggt acagggactg ctgtgccagt tctgtagcag aagcagcaga tgcagtagtc
     1801 ttgttctcat aagaagtctt tgcagcagcc atgtcgcttg ttgcaacgcc ctttgccttt
     1861 gcatagtaga tcaaagtgtt tgccagagaa gatacacaac ccaggtcacc gtcgccgtag
     1921 ccaacgatgg tgcaggtcag gttgttgtta tcctgatatg tgcccttcca atcgttcggc
     1981 tgaccagacc agatcaggct ggacgggatc gagaaggatt cgccgtcatc agttataccc
     2041 ttagacagat ccggaattgt aaactctgtt tcagtagtat catacggctc atactttgca
     2101 gagaatgtct tttcgccgtt tgccttaccc agaatggtgt tgtccatgaa ccagcctacc
     2161 cagcgatcca ggatcttttc cagagcttcg ctcagcttca gaccagtctt gccgacctgc
     2221 ggatcatttg cattgtcgct ctttacaaca taatacagtt cagccagacg ctgtacagcc
     2281 catacctggt taccgatcca gtggttggaa cccgggtcag cgtatacagg gtgttccaga
     2341 taagccatat tatagaaagt agcctgaccg gacggatact gctcataacg gccgttccag
     2401 gagttggtgc agccgccggc gatcgggccg tccttagaca tcagccacag gtacagttcc
     2461 atctgtctgc tcagagaagc ttcgaagtcc tctgtagcac cctgtgcttt catgcctgcg
     2521 ttcagtgcag aatcatttac cagtgcatat gcagccagcg ggttctgata gaattcgtgg
     2581 cagtgagatg caccgatctg ccatgcccag gagccgtcca ttgcaccgcc ccaggaggtg
     2641 taccagttca tcagataatg cttaccatta tcactgccgc ccttagccca cttgtcctgg
     2701 cagccaattg ccttgtagta cttatcgtac atattgttac gggtttcgtc acccatctta
     2761 ccagccagtg cagagatgga ctgaccgccg ccccacttgg agtcaacagt ctgatcgccg
     2821 acaccccatc tgtttgcatc gtaaacagcc tgaattgcac ggtcttctgc gtccggtgcg
     2881 ttggtgtaag accactgctg agccggattc tgttcggtgt tgaagaagcc cttcataccg
     2941 ccgttgccgt tcagaccata gcctacatcg tgcaggtcgg tacaagcgtg cggaatggtt
     3001 tcccagcagg attcctgttc accacgctgg aaggtgttga tcagtgtgaa ctgtctgctg
     3061 ccgcttgtgg tgacaccagt ctgcttgccc tgagtaccat tcttaccgcc gaagccatac
     3121 cagtcatcaa cgtcagccag ccagtgcatc aggtacagac ccttatcgct gccgtatgcg
     3181 gagcagaagc tctggtggat cgggttctgt gcggtgttgc cctgtgccgt atcggtcgga
     3241 tactgttcaa tctgacccca ctcgtcgctg taagtagcac tcagctggcc gcccttctgc
     3301 atgaagttgc cgccctgctg atccgggatc atagttgctt ccatggtctt ccaagcctta
     3361 gccagatcgc cgatggattc ggtagaagca tccttggtgt tctttacggt tacttcgccc
     3421 ttgcttgcat cgtttacgat gttatcacgc attgcagcag cccataccag gtaggacagt
     3481 gcttcagaag tagtttcgtg accgtagtcc ggagcttcga tgcacagttc ttctaccgag
     3541 tggaacggga tacccagacc accggaaacg gtgtttgtgc tggacatata gccgttttcc
     3601 acaccgttgg ttacaacgtc atcatacagc gacataaagc gtgctgcata agagtcatca
     3661 ccgtaagcct cgtccttagt tctggagccg gcagcagatg cagcagtcgg caggattgcc
     3721 gagcatgtca ttgcagcagc cagtacagca gccagaaatg ttttgctttt cttgaattgc
     3781 atgtttagaa attcccctct ctgaaagata aatgtaggaa atggtggaat gccgcaggct
     3841 cgcatccttt cttcatggca ggacccggcg aacaggcgac acataatttt gagcgtatcc
     3901 tttgtccatt tnnnnnnnnn ncctatattt ttatggcatc acatatcttt tccgtagttt
     3961 tttgtacttt cgccggtttt ccgtctgttt ttcacagccc tatcacacaa atatggcata
     4021 ctgctttttg cgatacgcac aagcttttct gccagttgca gaagcccttt tatccatttt
     4081 ttctatattt catgatatgg ttacattctg tatatgacaa caacatatta ttttgcgata
     4141 ataagaggaa ggtgcaaact gcctacaaaa tggctttttg cagaaaggag catgatatgg
     4201 ttgaaattca aaacctgacc aaatactatg gaaataaagc tgctctcaaa ggcatcagct
     4261 ttaccataaa ggaaaacgag gtactgggct ttctggggcc gaacggtgcc ggcaagtcca
     4321 ctacattaaa tattatggca ggtgtcattc ccgccaccgg cggcagcgtc aagatccagg
     4381 gctatgacat cgcccagcag cctgtcaaag caaagcgatg catcggcttt ctgccggaga
     4441 tcccgccggt ctatccggat atgaaggtgc gggaatatct gaacttcgct gccggtctga
     4501 aaggcattcc ggcggcaaaa cgcaagggcg aagtggaacg tgtcatggaa cgcctcaaga
     4561 tcactgacgt gcagaagcgg ctgatccgga acctctccaa gggctataag cagcgtgtgg
     4621 gatttgccat ggcgatcctc ggcgacccgc cggtgctgat cctcgacgaa cctaccgttg
     4681 gtctggaccc gacacagctg atggaagtcc gcaacctgat cctggatctg cggcggaacc
     4741 acgccattat cctgagttcc cacattctgg gcgagatcag tgctgtctgc gaccgcatcg
     4801 tcatcatcaa ccacggcgag atcaaggcag acgacaccat tgaacggctg gaagaagctg
     4861 acgattccgg tctggtggta aagctgaaag tacagggcag ttccagagaa gtgccgaagg
     4921 tcgccagaag tgtggacggc gttgtccgcg tggacaatat tcagtttgtc cagactggtg
     4981 tctatacata cgatgtggaa ctggaaaatg acggtgtgcg gaacgctctg ctctctgcac
     5041 tgctccagca cggtctggaa gtactggaaa tttccaccga aaaaagaaat ctggaacagg
     5101 tatttgtcaa gctggtcaat cagaagacca agcactccag tctggaggat ctgttgcagg
     5161 aaatggacga cgaagccgca caggaacccg cagcccagcc ggcagaggac aagtctgccg
     5221 cagatgctcc ggcggaagat gctgccggaa cagacggcaa gaaggaggac taaccaccca
     5281 tgttttcact atataaaaag gaattacaga gttactttct gtcgccgatc gcgtactttg
     5341 tcatcgcgtt ctttatggcg acattctccc tgacgttcat tcaggagatc ggtaatgttt
     5401 cttcatctac ctatacgttt tcgttctcct cgatctttta cgtcaacatc ttctatttca
     5461 tcttcctgct gccgctgctg accatgcgga cattctccga ggaacgcaaa aacggcacgg
     5521 aaaccatgct gctctccagc ccgctgaacg tcacgcagat cgtcatggcg aagtttctcg
     5581 ctacagcgac cattctgctg atcatgctgg cggcttccct gttctatccg atcatcacct
     5641 ccatttatgg ccgtgttatc tggtccagcc tgatctgcac ctatatcggc ttcttcctgt
     5701 ttggtctggt gtgcatcgcc ctgggcatct tcatctcctc tctgacggaa atgcccctgc
     5761 tggcgatcct gctgagcgaa cttgccatgc tgatgctgat cctgatggac aacctgagtg
     5821 tcaactcttt cctctccggc attccggtgc tctctgatgt attgagctgg ttctcgaacc
     5881 aaaccaaatt ctatgtattc tcccaaggtc tgatgcagtt ctccgacttg ctgggatatg
     5941 taacggaaat cgcggtattc ctgatctgga ctatcatttc cattgaaaaa agacgctgga
     6001 gccggaggta agctgctatg agtcaaaaca aacaaacaaa ccgtttccac aactggaaaa
     6061 acaccaaatt cgccggcatg gcagtgacgc ttgccattct ggtgctggtc attgctgtgg
     6121 ttctgaacat gatcgtttcc cgtctggact tcagctggga catctctccc aacaagcagt
     6181 acagcctgag ctccaccacg gaaaaatatc tggatcagct ggacagcgag ggaaaaactg
     6241 tagacttcta cattctgacc accaaggaaa gtctggaaaa cgatatgagt tccctcaccc
     6301 tctatcgggc actggaagct tatgatgccc acaagtcgat caacctgatc tgggtagatc
     6361 cggacaccga caacgacacc atggagaaga tcaactccga caatgcattc accctgtcca
     6421 ccggtgacat ggtctttatc tgcgacaacg tgaaaaaacg tgtccccggt tcttccatgt
     6481 ataccgtcta tactgatgat gacggcaaca ccaccggcga ggacttcaag ggcgaaaacc
     6541 tgattaccgg cacaatcaag ggcgtggcag aaaaccgtac accgactgtg tattttctgt
     6601 ccggtcacgg cgaaaagtcg ctggacagct acaccaagtt caccaccaac ctgaccaact
     6661 acaactacgc cgccaaggaa ctgaacctgt cggaaactga cagtgtgccg gacgatgcgg
     6721 ctctggtact ggtggcagca ccctccaagg atattaccga tgcagaagcg gaaaagctga
     6781 atgcatatct ggacaacggc ggcaacatca gcctgctgat gtcccccaac ggcggtgcat
     6841 tcgcctatac caatctggaa gcgatcatga aggactacgg gttctacatg gactatgacc
     6901 gcgtttcgga aaccaactcc aactatcaca tttccggcga caagtacacc attcagtgtg
     6961 aactgaacga actggacgac gacagtgaag ccaccgacct gacttcctct ctgctcaatc
     7021 agggactgta cacctttatg ccggaatccc gcagcttccg ctacaacgac gagggcggca
     7081 agtacaccat cgctcccctg atcaccacct atgacagtgc agagggcgaa ccttacggcg
     7141 gcgtcagcga cgatccggaa aaacaaagcg gacagatgct tctggcagca gactccacca
     7201 gcatcgccag aaacgattcc aaaatggtcg ttttcggcaa cgcggaattc attgatgacg
     7261 aacatgtcag cgatgatacc gttatcgttc cggtatacct gttcctgagc acgatcagct
     7321 ggatgtatga cagcgacatc gacatggaga tttccacaaa gtccaccacg aacgactaca
     7381 tttccctgca ggatgccgga ttcgccaagg ggctgatggt agtgctcacc gtcattccgg
     7441 tagcgatcat ggctgccggc attggcatct ggatcaaacg ccggaattcg taatccgttt
     7501 ctgccccaag gaggcaattg tacaatatga aacgacaact cattatcggg ctgattctgg
     7561 ttctgctgat cggtgtgtgc ttcagtgcct atttcggcgt ggactactac cagacccaaa
     7621 aagaggaaaa ggctgcagaa gaagcggcag ctctccagct gagttccttt gattccgatg
     7681 cagtaacaaa gctggtgatc catacaccgg aactggatta caccattgac aaggacgaca
     7741 acagccagtg gcaggttgcc gagggggatg cgatgcacat caatacctat tatattgatg
     7801 ccctctgcac ctacggctgt tctctgacag cgaccaagga tctgggaact gcggacagcg
     7861 acaagctgaa aacctacggg ctgtccgatc cgatttcgat cacctactac accagtgatg
     7921 cagacaagcc ggaaaagacc ctgtacatcg gcggtcagaa tcccaccaag tccagcttct
     7981 atgtgatgca ggacgatgac gaccatgtgt atctggcaga tgccaacacc gccggttatc
     8041 tctatgtcac caaaacgcag atgcgttacc gctatctcat ggacgacaag agttctgaca
     8101 ttctcaagct gaccttacag cggaacggcg aaacggtcta tacctttgag gacacttccg
     8161 gaaactctga ctacaaactg acagaaccgc tggaaacgcc gattgcggtc aacaacgcca
     8221 acctctctac cctgttcacc cagcttaccg aactggaagc tgatgatttc ggggacagcg
     8281 atgtgaccga ggacaagtat gccgagtacg gattcgacaa gccggcatac atgttccagt
     8341 ttacacaggc gaccggagaa gtcaccaccc tgctggtgca ggatttcgat ccgctgacat
     8401 cttcttatgt agactgtctg cacaaggaaa ccaacgagat cctgaaactg gactccagct
     8461 atctgggatt cctgcaaaaa aatgcttcag attacatgga cgacaccgtc tgctatttca
     8521 gcatcagtga ggtttccgca ctgaccatgc agtatcacgg ttctttcaat gacaagacca
     8581 tagacatcga cgacagcttc acatttgacg atgccagcac cacctacagc tgcaacggaa
     8641 aaagcttcaa cagcgacgac acggaactga ccgaggcgtt caagaccttc tacaccgcac
     8701 tgtccgagat ctcctacgaa tcgctggaca cttctgcaca ggctccgaca gataccgagc
     8761 cggctctcca cttggagtat accatgctgg acggcagcac ccatgtggta gaccttgtga
     8821 aaaaggacga caccacctat tgggtattca ttgacggaga attcacccac gccgttgtgc
     8881 ggcagcgtgc cttgagtggt gaagacaagg tgctggaaac ctacactgca ttccagaagc
     8941 ttctggaatc tgctgcggcg taaacgctgc attgaatcat tgcatatttc tctgcggaga
     9001 tgctacagtt ttgtggcatc tccgtttttt gcggcgagcg gcgagtcgcc gctgacctta
     9061 cgccccaatt tcaactgtaa aaagggtagt g
//
LOCUS       DAWBGR010000096         9047 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2139_length_9047_cov_5.146241, whole genome shotgun sequence.
ACCESSION   DAWBGR010000096 DAWBGR010000000
VERSION     DAWBGR010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9047)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9047)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9047
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2139_length_9047_cov_5.146241"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1967 a         2515 c         2384 g         2181 t
ORIGIN      
        1 ccgaaaaatc tgactacgca tcttacgcac aagctttatt cagtgtttcc ctaacaactg
       61 atatgcttct gcccacatct cgtcgatgac ttccttgcag ggcttgacat cgtgcaccat
      121 gccggcgatc agaccgcaca ggaacgagcc ttcgtccacg ttgccgtcta ccactgcctt
      181 gcggagagag ccgactgtca ttgcctcgaa atcctccggc gggcaggctg ccttttccat
      241 tgcctgcaac ttcttggtgt acttggtctt gatgccgcgg caggggctgc cggaatagaa
      301 gccggtgatc tggttgtcgg tgtcctttgc cttaacgacc agttccttgt acttcggaga
      361 gatctggcat tcctctgttg cgaggaaccg tgtgcccagc tgtacgccct cggcacccag
      421 cataaaggct gcggcgatgc ctctgccgtc agcgataccg ccggcagcaa tgaccggaat
      481 gtctaccgca tcgaccacct gcggcaccag acacatggtg gtgttcatgc cgatatgtcc
      541 gccggactca gtgccctctg ccaccagtgc atctgcaccg gaacgctcca tacgctttgc
      601 cagagccaca gacggcacca ccggaatcac cttgatgccg gcatttttcc acgcctccat
      661 gtacttgccg gggttgccgg caccggtagt cacgatcggc accttttcgt caatgaccag
      721 ctgtgccaga tcgtcagcgt tggggctcat gagcatgatg ttcacgccga acgggttctg
      781 cgttttttcc ttgcagatac gaatctgttc ccgcaggtaa tcaatggggg cactgccgcc
      841 ggcgatcagg ccgactgctc cggcattgga taccgcagat gccagtgtgc tctctgcgat
      901 ccacgccata ccgccctgaa tcatggggta cttgctcccc agcagttttg taattctggt
      961 ttccatagct atgttccttt ctatttcagg agatgttttc tcaatattcc ataatcagtg
     1021 ctgcataggt cagacctgct ccgaagccga ccagacaaac cttatcgcca cgctggatct
     1081 ttccggcacg gatcgcttca tcaaaggcaa tggggatcga agcactggaa gtgttgccgt
     1141 gatattcgat gttggtgatg actttttcca taggctgctt caggaacttc attgccgttt
     1201 caatgatacg gatatttgcc tgatgcggaa cgatcttatc cagttcgttc aggtcgaagc
     1261 cgatcttttc cgctgccatt gttgccgcct ttgccagtgc gtgggtggcg aacttgtaca
     1321 cctctctgcc gtcctgcagc atggtgtgtt cccagccctc cggacctgct ccgatgccgt
     1381 catcatattc tgcccgacgc tccggcttca caaagggcga ctgaggcagt gcactgtggg
     1441 catagaggaa ctttgcaccg gtgccgtctg cacccagatg gctggtgtac agagcatctt
     1501 ccttgtactc gatgactgcc gctgcggcac cgtccccgaa gagaatgcag gaagaacggt
     1561 cactatagtt ggtgatcttg gaaaggctct cgtttgcaac aaccagtgca taccgcatgg
     1621 aggaatccgt tttcaggaag cggtgtgcgg tgtccatggc atagacaaag ccggagcatg
     1681 ccgcattcat atcaaagcat gccgcattgg ctgcaccggt ttcccgctga atgatgcagg
     1741 acatagacgg ggtgcagtac tccggtgtaa tggtggtgtc gatcagcaga ccgatctcca
     1801 gcggatcgat gcctgccgca gcaatggcac gcttggcagc ttctgccccc atgtaccatg
     1861 tcggctcgcc gtctgaaatg tggcgggtgc cgatgccggt tctggtgcgg atccactcgt
     1921 cgttggtttc aacgatcttt gccatatcct cattggtcac ctgaaatgca ggcgtatagc
     1981 ttcctgttcc taaaacgcga attcccatgg aaaaatttcc tccgttcctc ttgtcacttt
     2041 cctcattttg tggtatgata gatagggaac gtaccgcaga cagaggtttc ggtacgccct
     2101 gtaaaattta gagtttccct gcaatcacag gatacacttt ctattataga atatttttac
     2161 gcaatttgca atgtttccga gtggtttttc aagtcgtgaa agaggaaata ctattttttg
     2221 cacaaatcta agcctctgat tttgtacatc tttgtacaac tcttatgaga aaggaatggt
     2281 agttatggca acaatcgcac ttttttccgg acagggttct caatatgaga agatgggact
     2341 ggatctggta cagcaggacg ctgcactgga ggaaattttt gaagtcgctt cggaggttct
     2401 gggcttcgat ctgtatgcgg tcatcgcaga cagcgacgca cagacactgg ctcacaccat
     2461 ttatgcccag ccggcaatca tggcaacttc tctgatctgc ctggaggcag ccaagaagca
     2521 tggtttcacc tatgacgccg ttgccggcca ctctctgggc gaatatgccg caatggtcgc
     2581 ttcgggcatg ctctctctgg aggacggctt tcgggtcatc aaagctcgtt cagaagccat
     2641 ggacgctgcg gcaaaggcag ccgacggtgc catgtgtgca atcctgaagc tggctcccgc
     2701 agaagtggaa cgcatctgtc aggaaacgga gggctatgtc attccggcaa actacaactc
     2761 cagcgtacag accgtgatcg ccggcaaccg tgatgctgtg gaaaaggcgg ctgccgcttg
     2821 tgccgcactg aaggcaagag ccgtaccgct ggctgtggca agtgcattcc acaccaagct
     2881 gatgcagccg gctgcggata ccttttataa agcgatccag aacgtgaagt tctctgctcc
     2941 gacggtgccc ttctactcca acgtgctggg tgctgtccgg acggacttct ccgatatgcc
     3001 caaggctctg gcggaacaca tcgtttctcc ggtacgcttc accgacgaac tggcagctat
     3061 gcaggcagcc ggcattgaca cctttgtgga attcggtccc aagagcgttc tcaccggact
     3121 ggtcaagaaa accctcaagg gcgtgactgc aaagaacgtg gaaaatgtgg acacactgga
     3181 aggagcattg gcataatgga acagtataca ctgatcggac acccgctggg acactccatg
     3241 tccccgttta ttcacgaacg gctgttcgcc atggcgggca gagaagccag ctataccctt
     3301 accgacattg caccggagca tctggaggga aaagagccct atctgcgtag cttacaggga
     3361 ttcaacatta ccattcccca taaaatggcg attattccca tagtggacga actggacgaa
     3421 agtgcaaagc ggtacaactc cgtcaactgc gtggcaaacc agaacggcaa gctgatcggc
     3481 tataacaccg actgtgacgg cttcaccatg tctgtgaaag agtaccccat ggacggcaag
     3541 gtgctgctca tcggctgcgg cggcgtaggc agaatgatcg ccaccgaagc cctgcggcac
     3601 ggggcgaacc tgaccattgg cattatcccg caggacaagg aactggtgga agcgttcatt
     3661 caggaggcaa aacagaccat gccggaagcc aaggtgacct atgctgtcac tgcggacatt
     3721 cacgacaagt ttgatgtcat catcaacgct tctccggtgg gcatgttccc caaagtggac
     3781 gcgtgtccgg tatctgatga actgatcgac ggggcagact atttcttcga tgtcatctac
     3841 aaccccacca agacgctgtt cctccgcaag gctctggctc agggcaaggt ggcaaagggc
     3901 ggtgccgcta tgctggtatt gcaggcagtc cgtgcccacg agatctggaa cggcgacagc
     3961 tataccgaca agcagatcga atccattatc tgtgacatgg aagaactgat cgacgaacag
     4021 aacgccaaaa agtgagggac agcatgacga tttttctttg cggcttcatg ggctgcggca
     4081 aaaccactgt gggcgagatt ctggcgaagc agctggggct gccgctgatc gacacggatg
     4141 cttatattgt ggaacaggaa ggcaggagca ttccggaaat ttttgctcaa gagggcgaac
     4201 cgtatttccg gaaggtggag gctcaggcaa tccgcaccct gtgcaccaaa aacgccgtga
     4261 tcgcctgcgg cggcggtgcc atgctgaatc cggacagtgc tgcctacgcc cgcgaaaacg
     4321 gagctgtggt actgctgaac gaatccttcg acacctgcta tgcacggatt cagggcgaca
     4381 ccaaccgccc cattgtgcaa cgcagcacca aagaagaact gcaccagctg tttgaccagc
     4441 gtgccggcat ctaccgtgcc cacgcttccc acgtggtaga cggcggtgtt tcgccggaac
     4501 aaatggcgga acgtgtcgcc gcagcggtac agtgaccgaa caccacacaa tagaatcaag
     4561 aaaacgccgt ccgacaggag catatgcctg ccggacggcg ttttttatcg ttatctgtcc
     4621 tgttattctg tccaactcgg tgtcagacct gcaccctttt cggcacagta tttcagaatc
     4681 atatacatat cggtggtgtc gatttttccg tccccgttga catctgccgc gtccagtgca
     4741 gctgcttcgg cagcttcgcc gtctgtggtc agagtgacag cttccttgcc ggaacctttc
     4801 cacgccagat actgcatgac ggcatagagg tcgctgctgg tgatcgcctc gtcattgtcc
     4861 acatcgccgg tatagtacgc agcagtcagg tctaccggct ctccggaagt ctgtatactc
     4921 tcggctgccg acatcagata ttccagcccc acttctgcgg cgggatatgc ctcctgcaat
     4981 ttttctgcaa gagcgatgcc gtcatagatg gattctacct tggtaacgtt gatcgattcc
     5041 caagaaatgc cgtcgctgta atcgatattt cggatttcca cttctgcatc agaatccact
     5101 gtccgtgcca gccggagcac tgcatctttt tcggattcct ccacattttt cacccagatg
     5161 ccgttatagt agttcccctc tgctcccatt gcaaaaactg cgtgataggt tgaccgaagc
     5221 agaacgccgg ctttctgcaa gtcacgggta agcttcagca cagcatctcg cttgttttca
     5281 aaatcagcgg gatttttgga atttacgccg tctgcattct tttcatcacg catgataatc
     5341 agccagttcg tatcactgat ctgaatctta tcgtattcgt cgaaacccaa tgccgcctga
     5401 ttttcggcgt agaccttttc aaatgtatcc agattggaaa ccagaattcc cgtatcatta
     5461 aacgcatagt gttcaaaaat atacagattt gccgattcgg cttttccgtc ggtatacacg
     5521 gtataggcat cgctgtaatt tgtcgccctt gccaaaaaag acttatcgtc caactcggtg
     5581 tatcccttgg gcggttccag actggtgctg taaaacgcca ccgcaccggc agacataccg
     5641 gaaacagaag ccgccgctgt ggttactgcc aacgcagatc ccagtatcct tttccatttt
     5701 ttcatcataa aaatcatctc ctttataata atgagggatt gcctttacac ttttattata
     5761 attattttat gcacatctgt caatatcaac agcagaattt tggcatcttc aacaagaaat
     5821 gatactctat tttgtacccc cacgaaaaca ttggagaaat ctggaaaaac gcaaaaagac
     5881 cgcacatctg aaatgcacgg tctgaatttt tctattcttc ttcatacacc ggaaaatctg
     5941 ccggatcata gggaatgtgg ctgcggtcgt agtagtccct gaccgcggca cggactgcct
     6001 gctccgccag cacggaacag tgcagcttct gttccggcag cccgtccaga gcctctgcca
     6061 cggcctgatt ggtgagagcc agtgcctgtg acaagggctt tccggtaatc aataccgttg
     6121 ccatggaact ggaagcaatg gcagtgccgc agccgaacgt ttcaaaggtt gccctggtga
     6181 tgatgccgtc ctgaattttc aggtaaattt tcatggtatc gccgcagcgg gcactgccca
     6241 cctgtcccac gccgtccgcg tcctccatga tgccgacgtt ccgcgggttc atgaaatgat
     6301 ccatgacctt tgcactatat aacatggggc tttctccctt cttccaagtc ccgccaaacc
     6361 ggcgacatgc tccgcagacg tgtcaccact tccggcactg ctttcaacaa gtattccact
     6421 tcttccggcg tgttctccgc agacagcgac agccgcagag agccgtgtgc cgcctcatag
     6481 ggcacgccca ctgccagcag cacatggctg ggctccagtg aaccggaagt gcaggcagag
     6541 ccggaggagg cacagatgcc ctttgcgtcc agcagaagca gcagggcttc gccctctacg
     6601 ccctcgaagc agaaattcac gttccccgcc agacggttct tccggtcgcc gctaagcagg
     6661 ctgtggggaa tggtttccag ccctgcgatc agccgatccc gcagaccggc aacataggcg
     6721 gcgttctgtt ccagatttgc acatgcttcc cgcagtgccg ccgccatgcc tgcgattgcc
     6781 ggcagatttt ccgtgccggc acgcatgccc cgttcctgtg ccccgccgca gatcaggctg
     6841 tgcagcggtg tcccccgttt ggcgtacagc acacccacgc ctttggggcc atggaattta
     6901 tgggcggaca gagagagcat gtccaccggc aattgcgaca ggtcgatcgg cacatgtccc
     6961 accgcctgca cggcatcggt gtggaacagc acaccctttt cacggcagac tgctccgatc
     7021 tctgcaatgg gttcgatcgt gccgatttca ttgttggcaa acatcacact gaccagacag
     7081 gtgtccggac gaattgccgc ctgcaccgct tccggtgtaa ccataccggt ttccgaaacc
     7141 ggcagcagcg tcacggaaaa gccctcctcc tccagctgtt tcagggtgtg gagaatggcg
     7201 tggtgctcga tcgcggtgga aatgaggtgc ttcttaccgg acctggcacc ggcataggcg
     7261 gcagaccgca gtgcctgatt gtcggcttcg ctgccgccgg aggtgaagta gatctctctc
     7321 gccgtacagc ccagacagcc ggcaatgtcc tgccgtgcct gcaacaaggc ttctgcggca
     7381 gcctgcccta tatggtgcag actggagggg ttgccgtagt tctgttccag acaggacagc
     7441 atggcacgca ctgccgcttt agagggcttt gtggtagctg cattatctgc gtatatttgc
     7501 aatggggatt cctccgtttc cttagaacaa aaagcgggga ttgccgctgg tttccggcac
     7561 tccccgcttt tctattgata accagttttc tctctcgaaa ttgctgttgg ttttttactt
     7621 gactgcctcg aagccctgct ccagatctgc cagaatgtcg tcgatatgct cgataccgat
     7681 agacagtcga acggtggtcg gtgtgatgcc ggctgccagc agttcttcct cagtcatctg
     7741 cgaatgggtc gtagatgccg gatggatcac cagagatttc acgtctgcga cgtttgccag
     7801 cagcgagaag atcttcaggg attccgtgaa cttctgtgcc tgctccttgg tgccgttcag
     7861 ttcgaaggtg aagatcgatg caccgccgtt cggatagtac ttgttgtaca gttccagatc
     7921 cttgccggtt gcctgcgacg ggtggttgac cttcttgacc atggggtgat tcttcaggta
     7981 atctaccacc ttcagagcgt tctctacgtg gcgttctaca cgcagggaca gggtttccag
     8041 cccctgcagc agcaggaacg aatggaacgg ggacagggtc gcaccggtat cccgcatcag
     8101 ggtggtgcga agcttcatga tataggcgat attgccgcat gcttctgtaa agacaacgcc
     8161 gtgataggac ggattcggct tggacagtcc cgggaacttg tcgcttgctg cccagtcaaa
     8221 cttaccgccg tctacaacaa caccacccat cacggtaccg tgaccgccga tgaacttggt
     8281 tgcggagtgt accacaatat ctgcaccgtg ttccagcgga cggaacagga acggtgttgc
     8341 gaaagtgttg tccacgatca gcggcagacc gtgcttgtga gcgatctccg caatggcttc
     8401 catgtctacc acgtcagaat tcgggttgcc cagagattcc acatacactg ccttggtgtt
     8461 cgggcgaatg gctgcttcga tatttgccgg atcgtgtgca tctacgaaag tggtttcgat
     8521 accgatctct gcgtaagtgt ttgcgaacag gttataggtg ccgccgtaaa ccgtctgcga
     8581 agaaacgata tggtcgccgc tgcctgcgat attgcgaatg gaatagtcaa ttgctgcgga
     8641 accggttgcg gttgccagtg cagctgcacc gttttccagt gcggcgatac gcttttcaaa
     8701 aacgtcgttg gtcgggttca tcagacgggt gtagatgttg cccgattctg tcagaccgaa
     8761 acggccggct gcctgtgcgg agtccttgaa cacataagaa gtggtcgcat agatcggcac
     8821 tgctcttgca tcagtggtcg ggtccggctg ttcctgaccg acatgtacct gtttggtttc
     8881 aaaacgatat tcctgatttg ccatggtgaa tctttccttt ctatttcgtc cgtgccctgc
     8941 gggcattcag gaaacactgc ataaagcttg tgcgtaagat gtgtagtcag atttttcgac
     9001 agattgcata gaaattccgc aggcagactg tatgtctggc aagggat
//
LOCUS       DAWBGR010000097         9029 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2141_length_9029_cov_3.213060, whole genome shotgun sequence.
ACCESSION   DAWBGR010000097 DAWBGR010000000
VERSION     DAWBGR010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9029)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2141_length_9029_cov_3.213060"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    558..567
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1746..1755
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2988..2997
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2430 a         2229 c         2139 g         2201 t
ORIGIN      
        1 caaaacaatt tcatcgtagg atatgctttt tttaacgtag tatttatgct atactaaaat
       61 tataccacaa agaactacgt gaaatgcaca agcgtgttcc tataaaatgc ttgtgaacat
      121 actttgagtt atgagggatt ggaaaatgtt aagaattgtg atttttgatg atgaggaacg
      181 catcggactg caattaaaag aactcttaga gcaatctggt gacgatctgg ttgtggattt
      241 ttttcaggac tggcatgccc tgcaatttta tttttcgcag catgcacata ttgacgctgt
      301 atttatggac atctgctttc agggaagcac cagcagcggc atcgatttcg cggaaaaaat
      361 tgcacagtcc cattcacaaa cgaagtttat ctatgtgaca ggacacccca gggaatattt
      421 gcagcagatt tttatgagca aaacagtgaa cccgttcggc tatctgccaa agccctttga
      481 ccggaaggcg gtgcaggatt atctgcaaaa aattcgggca gcatcggcag acagtcagga
      541 tctgttccct ttcaaacnnn nnnnnnnccg aaaaagggac aggtggtcta cctctctctg
      601 cgggaagtgc tgtatttttc cagcgacaga cgctgcgtca cagcacacac ccttcacggt
      661 tcctatgagg gctactacca gctttccaag ctcatggaga gcctgccgca gtacttttat
      721 cagtgctata aaagttatgc tatcaatctg cactatgtac agcgaattga caccaaggaa
      781 gtacatattc aggaggagat cattccgatc agtgcatatg ggcaccgctc tgttgcagaa
      841 aatcgggcag agttggtgca acgtaaaagc gaactgtatc aatcgtaaaa catacggcaa
      901 taccgcacaa aggagtccta tagaaaatgg ataatttttt ttacaccatg aattttacgg
      961 tgtcgaatct gctgctatat ggctttttgc tgctgtatag ccacatctgt gtgggaaagc
     1021 cccgaaaagg ctgggcattg gcgactggtt tgatcatcct tctgattgac ctgatcttcg
     1081 caattatccc gtgggttttg tacggagatg cttcctgttg gatcattcag acacaccgca
     1141 atgggttcac tgctctgcac tggtgcatct ttctcttgtt tttcctgacg ggatttcccc
     1201 aagaaaaact cctgactcgt ttttttctcg caacgatgtc atttttattc atctttctgg
     1261 gagataccat acagatcatc gccatagctg ccatagacaa tcttgccgga accaatgtct
     1321 gcagcattgg ttccaactat gaaaactggc atctctggta ccgcacattt ctcatactgc
     1381 cggaactgtt aattatggct ggtctggcgt ttgtgttcca aaagctgttt ctcaagatcc
     1441 gttcacagct gtattcagct aagacgttct ggctgctgat ttttccggtg acacagtttt
     1501 ttgccattta caccctgatc gcaattttac agcaattcgg cacgatcagc acctccccga
     1561 agattctggc tgtgctgatc gttacgattc tgctgaatgt tgcttccaac atcatgctgc
     1621 tgcatctgat gaaaagaatg cacatcaaag aacaggaaaa gcagaaagtt cgtttttatg
     1681 aagaatacga aaaactgtcg gcaaaatatc aggaacagat cgcacaggct tctcacgaac
     1741 aggaannnnn nnnnnaaaat ctgaaacgag atttccacaa ccagatgcag gtcttttccg
     1801 gattgatgca gactgaccat gtggaagatg ccatgcagct ggcagaggaa ttgcaaagca
     1861 agttccggca gcagaagcaa cggctgtatt tctgtgaaaa cactgtcgcc aatgtgattt
     1921 tacagcagac cgcagaaacc tgtgcaaaag agcacattgc atttaaaaca gactgtgctc
     1981 ttgcggaaga catcggaatc cgcaaggttg atctgtgcag cctgcttatc aatccgctgc
     2041 aaaacgccat tcaggcatcg gaagctgttc cggaggcaga acgagaaatt tcctgcacta
     2101 tctggcagga tggagaaatg gtattcctgc gaatctgcaa ccggaaaaat caccagatcc
     2161 agaccaaaaa cggaaaagta cagactacaa aagcggacaa ggaaaagcac ggatttggta
     2221 ttgaaattat agaacatctt gccaatactt acggcggaat agctgtcgcc agctatgatg
     2281 accagttttt cacagtcacc attcagctaa atctgccggc agagaacaac acagaaaaaa
     2341 ttgcagagga ggctgctgtg gtatgtccgt aactgaatcc atctgggcaa tggtgaatct
     2401 gacgctgtct gctttggcag tgatctggct tactgcttcc tatgccaaag tcgcgttcca
     2461 aaagacgaac aaacgggcaa atctcctgct gcttggctat gctgcatttt tgacgatctg
     2521 ttttttcgca gtttcttacg gcgtgtatgg cgatgtgaaa tatctgattc tggaaaaggc
     2581 ccggtcgctg tactccctgc tcttttggac ggcaatcctc ttctactaca tctttgcgta
     2641 tcgaaagcag cccctgttca agcggatatt tcacagcatc atctgcggca ttgcaatggc
     2701 tttttccgat attatcagcg tcactcttct cactactttc gactttatcg ccaagaccga
     2761 tttttgttct ttgattttga gcgatgagcg ttggagccac tggtattcct ttttggggta
     2821 tattcccacg ccggttattt atttcacgtt tctattcttc tttcggaaag ttcttcaaaa
     2881 aaggcggctg catctcactt ttgaaagcac aaagcaaaat ctgatccttt tgatttttcc
     2941 gattgcacaa gccgctatca ttgctacatt taccatcatt attcagcnnn nnnnnnnatt
     3001 cagcagcata aagttctcac gtcctctccg ttgattctgc ttatggtggg aatcacattt
     3061 ctccttgaca tgatctccag catttttctg ttccggttca tacaaagatt acagaacgag
     3121 gaacggctca ccagaaagct gcaatttttt gagaaatacg aaaccctttc cctgcaattt
     3181 caggaacagg cagcagaagt ttcgcgggaa acagcgaagc tgcgtcacga ctttaacaac
     3241 cagatgcagg tctttttcgg attggtcacc tccaatgcaa tcgacgatgc aaaacagttt
     3301 gccgccgaat tggaggagaa ctttcagaat ccggaattgc agctgcaata ttgtgacaac
     3361 gagatcatca atgtgatcct gcatcaaacc gctgcgaaat gtgcggcaaa acaaattgca
     3421 tttcctgtag aatgcgactt gccaaaagca ctgccctttt ccaagttgga tctgtgcagt
     3481 cttttgatga atccattgca gcgtgcagaa cagctggtgc aaaccgtttc agaggcagag
     3541 cgaagcatta cctgccgcct ctgggaggag aacggcaatt tctgcttccg cgtgcagaat
     3601 cccaaaggcc ccgcatctgt ccagcctgta ccattggata ctgccgcaca gatcgccaac
     3661 agctatcacg gtacactgac actatcggaa gagaccggct gcgaagttac ggtacaaatt
     3721 ccactttcct aaggagttac tatgcgatat caagaagtga tccctgccgt ttttctggaa
     3781 cgcccgaacc gattcattgc actggtactg gtcaacgggc aaaccgaacg ggtacatgtc
     3841 aaaaatactg gtcgctgtcg ggagttgctg attgccggct gcacggtgta tctggctgcc
     3901 ggcaagaacc ccagccgcaa aaccaaatac gatttgattg ccgtggaaaa ggtgctgccg
     3961 gattccacgg cattgctgat caacatcgat gcacaggctc ccaacgccgc ggcggaggaa
     4021 tggctgcaca caggggcact ctttcctccg gcagctgaaa tccggcggga agtgacgtat
     4081 gggaattccc gctttgactt ctgcattacc cacggagaaa ctaccgcgtt tctggaagtc
     4141 aagggtgtga cactggaaca aaatggtgtc gtttcctttc ctgatgctcc gacagaacga
     4201 ggtaccaagc acttgctgga gctggctgcc tgccggcaga aaggctatga agcatacctg
     4261 ctgttcgtgg ttcagctgaa aggtgctgaa cagttccgcc ccaatgacag tatggatccg
     4321 gcatttggaa aagctctccg gcatgctgta gattccggcg tacaaattct ggcaatggac
     4381 tgcgttgtca cgccggattc tatgacgatc gatcagccgg ttccgatcat tcttccctac
     4441 gaaaaataat cctcaatcag ccgctgcaag gctgcttcat cggacacagt aattccctgc
     4501 tccaatgcgt gccggtcata gtcggacagc agattcagcg gaacttgcag tttctgatac
     4561 ggcacactgc tgccctcccg aaacagtccc acctgcccgt ctgtgatacg catggtatag
     4621 cctgcttctg ttgccgcttc ggaataggtt gtcccctgtg ctgcactttt ttcctgccgc
     4681 agagataggg acagcgagcc gcctacgatc accagcgata tggtgagagc cgtcagcaga
     4741 atccacactg catttcgttt ttccattttt ctcgtccgcc tttctgtttt cacatcgcag
     4801 aacaaaacca gatttttctg cgaatttgcc tgaatatata ccaaaagtat tctttccaaa
     4861 attccgaagt ttatacaatc tgcatatttt tcaaagaaaa tccgccgtac ctcttaacat
     4921 tgcccgtaaa atgtgctata ataatatggt atacattccg gcagtgtgca tctcggacaa
     4981 tgctgccgat acgtcttacg tacaaattta ttttgtgtgg tactgctcct gcaaaacaac
     5041 aacagattcc cctgtgtgta ttctgccgca cagcgggctt ttggaaggaa tcaatactat
     5101 ggataacaac aaaagacgaa acagttctcc ccccactgac aaccgaaagc gagccaaaaa
     5161 aggcgggaca ggcattccga tgcggaaata ttacggaccg accggtacac cggaatatcc
     5221 ctatcacagt ccggaactgg aagaagccgc tgccaagccc aaatgggtcc tcgttctgcg
     5281 gcaaacgctt tctattctgg gttccacact tgcggcgatg ttcatgatcc tgatcatcac
     5341 ctgcaccatt gtggcaacag cactggtcgt ttatgtcatg aactttcggg acgatgtgtc
     5401 caatgttacc attgaagaaa tggaactttc ctacaatacc aacatctatg ctcaggacag
     5461 cagcggcgaa tgggtgaaca tctatgaagt caccaacgaa tcccagcgta ttcccgttaa
     5521 catcgacgag attccccagc atacgcggga cgcttttgtc tgtgcagaag atgaacgttt
     5581 ctatacccat gacggcgtgg actataagcg taccgtttcg gcctttgtca acatgttcgt
     5641 acacatctat gataccaatc agggtggttc gaccattacg cagcagctga tcaaaaatat
     5701 caccggtgac agcgagcagt ccccttcccg taagatccgt gaaattttcc gtgccatgga
     5761 actggaacgt gcttactcca aagataagat tctggaaacc tacatgaact acatcggttt
     5821 cggcggcacc tccaacggaa tcgaacatgc cgcacagaaa tatttcggaa aaagtgccaa
     5881 agatctggac gtagcagaag cggcttgtct ggcagcaatt ccgaaaagtc cagaaacact
     5941 gaaccccttt gccggatata aagatgatac caccggagaa tgggtcaata ccggcaaaaa
     6001 aaagaacaaa aagcgacagg aatatgtcct ctggcagatg tacaacaacg gtgctctcac
     6061 ttatgacgag taccagtcgg ctctgcatga gaagctgatc ttcacagact ccgacgagta
     6121 caaggcagct caccctgatg cacaaaagga agaagacacc gatcccaaca agcccacctc
     6181 ctggatcgta gacgcggctc tgtatgaagt ggcagattac ctcaaggaca agttcaatct
     6241 gactacacag caagccatct cccgtatcaa ccgcggcggg tatcagatct ataccacggt
     6301 caatcaggat atgcaggctt atgtggagga gaagtacaag gatctaaaca atctggtgga
     6361 aaaagaccgc gttgcccagt ggcaggatca ggacggcgac ggcgaatact cctccagtga
     6421 aatcgtttat ccggagtctg cttttattgc aatgaactat aagggcgaga ttcaggcaat
     6481 ggtcggtgca gtcggggaaa agacagaatc cctgtgcttc aactacgcca ccatggaaca
     6541 gcggcagccc ggttctacca ttaagccgct gactacctac ggactggctt tggaaagcga
     6601 cctgattcac tggggctcta tttacaagga cgagccgatc gaagttgagg gcaaggcatg
     6661 gccgaccaac tacagcgagg acagttctgc catgtctatc agccacaagg aactgaagat
     6721 atatgaggca ctggaaaaat cctacaacac tgtaccggct caactttgtc aggctctgac
     6781 accgcagagc gttttcgact ttgccacctc caaaatgcgt ctggatctgt gtaaagattc
     6841 cggcgacgga cacaccgata tggcatactc tccgctgaca gttggtgcgt tgacatatgg
     6901 tgttacactg gaaaatctgg tcaatggcta tgttccctat ggcaacggcg gtacacagta
     6961 tcaggctcac cttgtcagca aggtggtaca aggtgccggc gacctcattt atgaaaatga
     7021 cggcagtccg gaagaagctg tcagcagcga aactgcgtat gtcatgaaca agctgctgca
     7081 ggatgttgtc aacaatggta ctggtcagaa ggcaaagctg gaacacaagc atgttgccgg
     7141 taagacgggt actacacagg actggaacga cctgaccttt gtgggactga cagaagattt
     7201 cgtcagcggc gtatggctcg gctacaagga acgtgcagaa ctgaaagacc accatatcaa
     7261 gtctgcacag atctggcaga acatcatcgg tgaatatgcc gaccagatgg acaccggtgc
     7321 ggaatatcct gaagacagca gcatagtaga aggtaaggtt tgtaccaaga gcggcaagat
     7381 cgctggtccg aactgcaccg ccaccactac cggctactgg aagtccagca atgctccggt
     7441 ttgtgatgag tgcacaaagt acggcagcaa aacgacaaag gctaccaatg aaaacagcgg
     7501 ttccacatcg gcaaccactg ctgcgggaga acagcatagt gaaacccagc atccggcaga
     7561 aaacaatagt aatgctgccg caacagaagc agctgccaca caggcaccgg ctcaataaga
     7621 accattttta aatttcatac accgcacaga accagctgtt ttgtgcggtg ttattgtgaa
     7681 ggagtatttt tatgatgcaa caatcatctt acctgctttg ctgcccctgc cattttgggc
     7741 tggaacgcac gctgcggttc gaaatttcca aaatcggcgg cactgacatt gcagctcagg
     7801 acggtcgtgt tctgttccgc ggcaatgccg aaaccattgc caaggcaaat ctctgcctgt
     7861 ctacggcgga acgtgttctg attcagctgg gacagttcaa ggcgtacaac tttgaagaat
     7921 tgtttcaggg tgtccgttcc ctgccgctgg aacaatggat cggtaaggac gatgcttttc
     7981 cggtaaaagg acatgctctg gactccaagc tgcacagtgt gccggactgc cagtctatca
     8041 tcaaaaaggc agctgtggaa cggctgaaat cggtatacca caccaattgg tttacagaat
     8101 ccggtgcggc tcacccaatc cagtttacca ttcgcaagaa cattgccacg atttatctgg
     8161 ataccaccgg aatcggactg cacaagcgag gctaccggag aaattccaat gctgccccga
     8221 tcaaggaaac gctcgccgcc ggtattgttg acctcgccag agtacattcg gacagcattg
     8281 tctgtgaccc attctgcggc agcggtaccc tgttgatcga agcggcattc aaggcaatgc
     8341 gtatggctcc ggggctgaac cgccgctttc tggcagagca atgggcacag attcccaaaa
     8401 ctgtatggca gaatgcccgt gcagaagccg ttgcggcaat ccggcatgat gctgctttcg
     8461 aagcatacgg ctatgacatc gatccggagg caattgccct gacagaagaa aactgccgta
     8521 aagccggcgt tcagccgcgg attcatctga tgcagcggga catcgccgat ttcgaaccca
     8581 tagagcatgc cattaccatt tgcaatccgc cttacggcga acgcatgctg gacattcagc
     8641 atgcacagtc tatttaccgc atcatgggtg acgtgttccc tgccgacaag gctcacccat
     8701 gctacattat cacgccggat gcagattttg agctgcattt cggaaagccg gcaaacaaaa
     8761 agcgaaagct gtataacggt atgatccagt gcaatctgat gagttattat caggattgag
     8821 aaaacgctcc cactgacgaa aatggtgtca gtgggagcgt tttgcagttg tgggatttta
     8881 ggcaataccg cacaaagatt gtgcgacaga tgcgtcgtca gatttttcgg cagattgccc
     8941 agaaattccg caggcatact gccgtatggc aagggatttc tgggcaatat gacggaaaat
     9001 ctgcacgcag atggcgtgca aagtcgcca
//
LOCUS       DAWBGR010000098         9019 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2144_length_9019_cov_3.377287, whole genome shotgun sequence.
ACCESSION   DAWBGR010000098 DAWBGR010000000
VERSION     DAWBGR010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2144_length_9019_cov_3.377287"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2162 a         2229 c         2082 g         2546 t
ORIGIN      
        1 gcataatcta ctacgaatta tatactattt tttttcattt gtcaagaaca tcacggaaaa
       61 atgacatttg cacggaattt cgcccttttt tcgcacgttt tttatggaaa accacataaa
      121 atctcggtaa aaaatcggaa atggaattga aacgctgttt ttttgcttca gtagagaaaa
      181 agcttgtatc tgcgtgtcgt tttctgcttt ttacaatttt ctgcgtgaaa aaaatagctg
      241 ccggcagaaa actggctttt gtcaggcatt tgtgcaatat taccaaaaga aatgatgttt
      301 catattttat atattgttca taaaatatct attgcaatta aataaagttt cctgtatact
      361 aaacacatga ggaatcgttc cgatgaaatg attcacttcg aatacggaca atggcgtccc
      421 gtacagtcgg ttgctgtgcg agggcgtttt tttataccct ctgcaatcgc tttgaaggtg
      481 gaacaatggg gttgccgcca gaattggcat cggttttttc ccttggtgat gtaacatcta
      541 cgaattgatt ggatatggag gacatttgaa ttatggattc tgggaatatc ggctttgtta
      601 tgatttgtgc tgcacttgta ttttttatga ctcctggttt gtcgtgtttt tacggcggtc
      661 tggtgcggcg gaaaaacgct gtgaacacga tgttcacctc ggttgtcacg attgcgacgg
      721 gaatcgtgat gtggatgctg ttcggctact cgctttcgtt cggaccggat cacggcggtg
      781 tgatcggtga tttcagctgg tttggtctgg agggtgtttc cctgacagag ccatacatag
      841 agggacaggg cattccgaac atggtattct gcctgtttca gatgatgttt gcagtcatca
      901 caccggcact gattaccggt tctgtagcgg gcagaatgaa attcaagtcg ctgtttacgt
      961 tcttggtgct gtggagtttc gtggtatatt atcccatggc acatatggtc tggggcgacg
     1021 gcggtttctt ggcaaagatc ggttctgtag actttgccgg cggcaacgta gtacacatca
     1081 gttccggtat cacggcactg acactctgca ttctccttgg aaaacggtat gactataaac
     1141 gtgcaaacta taagattcac aacaccccga tggtggcact gggagcattt atcctgctgt
     1201 tcggctggtt cggattcaat gccggttctg cactggcagc agatggtctg gctgcacatg
     1261 catttgtaac caccgcagtt tcttccgcag ccgcaatgct gtcgtggatg ttctgtgatg
     1321 tcgttgtcaa taaaaagccg acatttgtcg gagcctgcac tggtatggtt gccggtctgg
     1381 taggcattac accgggagcc ggttttgtac cggtctgggc ggccttgatt attggtctga
     1441 ccacttcgcc gatctgcttc ttcatgatct cttgggtcaa ggaaaagttc ggctatgacg
     1501 atgcactgga cgcatttggc tgccatggtg tcggcggaat ctggggcggt atctgtaccg
     1561 gtctgttctg caaggtgtct atcaacgaca tcggccaggg caatggtctg ttcttcgggg
     1621 acactcatct gttcttggca cagctggcaa gtatcggtat taccattgta gtggctgtag
     1681 ccggcacact gatttgctat ggtatcacac gtctggtaac cggaaagatt cgcgttaccg
     1741 aacgggaaga gcgtgtcggt ttggatcgct cgcagcacgg cgaatctgca tatccgtcgt
     1801 ttaacggctt agactaaaca aaggagtgac agcaatgatt attaaagtag aagcaatgat
     1861 tcgggaagaa aagctggagg acgttctgga cgctctggca gcattggacg tgcacggcat
     1921 gacttgttat caggtcatgg gctgcggtac ccagcgtggc tttaaggagt atgcctacgt
     1981 tcgcggacac cagcaggtag agattcagat gctcccgaag atcaagatcg aagtactggt
     2041 ttcgtcagaa gaatgggaga agaaaaccat tgatgccatt cagaaggcag cctataccgg
     2101 taatatcggc gacggcaaga tctttagcta tgatgtgcgg gaggcaatgc gaatccgcac
     2161 caaggaaacc ggctatgatg cgattcagcc ggaataaacg ggcgatataa ccaatagaga
     2221 agaacaagca tctcctggct ttgctgggga atacgattaa gggcaacacc gcacaagttt
     2281 tgtgcggtgt tgcccttatc atcttccgcc aaattgggaa ggctttaaaa cagtattgcc
     2341 tttataagga ttccgtctga aaatccggcg tataccctgc tgaagcagac gagcgttcct
     2401 gtccataacc gttcaatccc agctgcacag catgtttccg aacggactca tattcaaacg
     2461 tggcaattcg ccggttcagg tgcttgtccg acagcggcat ttccggcggg gtgtactgtc
     2521 ccatcagact tagcaaaaac gcatctgtag gcagagccgc cgccagccag tccagcagct
     2581 gcattgattc ttttcgatgt gccggcagaa ccagatgccg taccaccaga ccgcgttcca
     2641 gcaaatcgcc gttccatacc ggctttccca cctgtcggaa catttccgga atcgcctttg
     2701 ctgcaactga aaaatagtcc ggacagttgg catatgctcc tgcggtttcc ggtgtgtaaa
     2761 attttaaatc agggaggtag atgtccacca gtccctccag catgtgcagg atttccggcg
     2821 attcatagcc gccgcagttc cacaccaccg gaatgtgcag cttgggtttt gccagttcca
     2881 gagcctgcac gatccatggt gtataatggc ttcctgtgac cagattgata ttgtgtgcct
     2941 gctgttcctg caatttcaga aaaatctctg caagctgtgt cactgtgact gcttttccaa
     3001 cagcctgccg gctgattttc cggttctggc aaaatacaca acccagagca cagccggaaa
     3061 agaacacagt tccggaacca tgtctgccgg aaatgcaagg ctcctcccat gggtgcaggg
     3121 ctgctctggc gattcgaatt tccgtgccgc agtgacaaag ccccacgcct ttttcccgat
     3181 cagcaccgca tcgtctgggg cagagggtgc agtgtgccgc atcaaaactc atgacagccg
     3241 tccctcaaaa tcggcatagc caaaccgctt taccaaatcg atatgtccat tctgatagag
     3301 ggcaatgtcc ggcaagggaa cgccgttgaa tgtgttgttt ttcaccatgg agtaaatcgc
     3361 catgtcgcag aataccagct tttcgcctgc ttgcagctga tggtcgaaag agtaatctcc
     3421 gatcacatca ccggcaaggc aggtgtttcc ggcaagtcgg taggtatgag ttttttcgcc
     3481 ggctgtcccg ctgtacagaa tgtgcgggcg atagggcatt tccagaacgt ccggcatatg
     3541 gcaggcggca gaagcgtcca gaatggcaat gtccatgccg ttgtgtacca cgtccagcac
     3601 agttgccacc agataaccgg cattcagagc aactgcttct cccggctcca gatatacctg
     3661 tacgccgtag gtgtcccgta cccgttcaat gcagcgtacc agccgttcca cgtcatagtc
     3721 cggacgggtg atgtggtgtc cgccgccgaa gtttacccac ttgcatcgtt tcagccactt
     3781 gtcaaactgc ggcagcatgt ggtcgagggt acgctccaga acgtcagagt tctgctcgca
     3841 catggtgtga aagtgaatcc cgtccaagcc ggcaaagtcg tcttccattt gttccagcgg
     3901 tacacccatg cgggaaccgg aaatgcaggg gttgtataga tcagtttcta cttctgcata
     3961 gccgggattg atgcgaatgc cgcaggaaat attccgggtt gcatcttgca cctgctgccg
     4021 gtattttttc cattgtccaa acgagttgaa tacaatatgg tcgcagagtt ccagcagctg
     4081 tgtgaaatct tcctgcgtgt aggcagggga gaacacatgc acttctttcc cgaagtgctc
     4141 tcgtcccagt tttgcctcga ataatccgct ggcagtcgtt ccggcgagat cgtcccgaat
     4201 cagcgggtag gcggagaaca ttgagaacgc cttttgtgcc agcaggatct tacagccggt
     4261 tcgctgctgg atttgtttca gcaacgcggt gttcttttgc agcagccgtg catcagtgag
     4321 atagcaaggc gtatgcagat catcaatggg aatgtcaaat aaagattcaa agtccatttt
     4381 caaaattcct ttcttgcaaa agaagcgttc tgcttgatgc ggaacgcttc ttctaatttg
     4441 agggattcat agattgcgtg caaagccgtc aggcgggctt tgtgcggtgt tgccttaaat
     4501 caggctttat gcagtgtttc ctttattttg gatcgggaaa ataccgcaga ggcagcattt
     4561 cttcccgtgt acaggaatcg ctaagaagtt ctaccaattt cgtttcggaa aactccgaat
     4621 gattctgaaa gattctggaa tacacttcct gtacatcact ttccacgtca tcagactgaa
     4681 aacagtccag atagtctaca acagtgcaat gtacagtgtc gccgtacata tttctacgct
     4741 tgttgtccat tgtttgcttt acatagattt ccgccttttg ataagcttca tcaaaagagc
     4801 cggcatttac ttgcaagata gattcctcgt agaaaatctg ttcctttaca gtgtatttca
     4861 gaagtagttt tacatggtac attgttgtat ctccttaatc taccagcacc ggatcaaaat
     4921 tctcagtcca cggcagcccc cacttgttca gtgcgtccat gaacggatcc ggatcgaatt
     4981 cttctacatt ataaacgccc ggttttttcc acttgccggt cattaccagc attgcaccga
     5041 tcattgccgg aacacctgtg gtgtaggaaa ctgcctgaga gccaacttcc ttgtagcatt
     5101 cctgatgatc gcagacattg taaacgtagt agttcaccgg cttaccgtcc ttgacaccct
     5161 gacaaatgca gccgatgttg gtcttgccca cggtgcgggg accgagagaa gccggatccg
     5221 gcagaactgc cttgaggaac tgcaggggaa cgatttcctt gccctcgaac atgatcggtt
     5281 cgatagaagt catacccaca ttttccagac acttcagatg cgtcagataa ctctgaccga
     5341 atgtcataaa aaagcggatt ctcttgatac ccttgatatt cagtgccaga gattccagtt
     5401 cttcgtggtg cagcaggtac atatcctttt cgccaacctg atcgaagttg tattctcgct
     5461 tgatttccat aggttcggtt tccacccatt cgccgttttc gatgtagctg ccctttgcag
     5521 aaacttcccg aatattgatt tccggattga agttggtagc gaaaggatag ccgtggtcac
     5581 cgccgttgca gtccagaatg tcgatataat tgatttcgtc aaagtagtgc ttctgggcat
     5641 aagcacaaaa tacgccagtt acgccggggt caaagccgct gcccagcaga gctgtgatgc
     5701 cagccttttc gaagcgttcc cgataagccc actgccactt gtactcaaac tttgccgtat
     5761 cttccggttc atagtttgcg gtgtccacat agtccgtctt ggttgcaagg caagcgtcca
     5821 taatggtcag atcctgatag ggaagtgcca cattgatgac aatgtccggc ttctcctgct
     5881 cgatcagagc gatcagctgg tctacatcat tggcatccac ctgtgccgtg gtgatcttgg
     5941 tagccgtctt gtcctgcaat tcttccttga acttgtcaca cttggacttg gtacggctgg
     6001 caatgcagat ctctgtaaac acttcactgt tctggcagca cttgtgaatg acaacgcctg
     6061 ccacgccgcc ggctccgata attaacgctt ttcccatgtt catatacctc caaaaattca
     6121 aaaataacaa tattgattag cagcaatccc aggtataaag cacatcactg aaatcgcagt
     6181 tttttaactt ttcccaattg atgaagaagt tgccgatgcc gcaatcaccc cataaaattt
     6241 tccagtcggt tcccatgtca ctgtccaact gaaacagcaa tacatcatgc gtgtcttcac
     6301 tgcttctggg gtcccattgt gcaaagccgg ggtaaccgcc gatcttatga ccgctgcccc
     6361 cgtcgaaaaa gtcatccata ttttctagca ggttttcagc ctcctctgga taatcctgtt
     6421 gtaccagttt tcgaatacag ctttcaaacc gataatcact gtaagaaatt gtgtccacat
     6481 cttccgagaa tgccagccca taacagcctc tcaccgggaa ataatcttcc tcatcagaag
     6541 acgaatcctg tactttttcg cagatttctt tctctgtaac tgtccgatcc ggttccttga
     6601 aatagagaac ccgaaacgtg tcctgcaagg tgttgtcatc gaaattcagt ccgctgacat
     6661 cgtcgttcag aatccagaat tgcagcagcc cctgatgcgg aaagtcttcc agcgtgatct
     6721 ctgcacagtc gatctgtgcc agtagtcgca gttgcacacc ctcgctgtcc tccggaattt
     6781 tcgcatcgcg gggaacatat ccaataccgc caaccttgct gtcaaacaga ctgggcttgg
     6841 tatctgtcag ctgcaatttc atatagggga ttgccgtctt ttgcagaatc tcctcacgga
     6901 tttctttcac catattcgca ctagaatccg ttttttcaca tgtttcgact ttctccggta
     6961 cactgtcttt ttgtttaaat atatccaaca gtcccatctt tgctccttat cttttgtcac
     7021 gctcagattc ccgcagagca tcgcgaacgt tgttcggaat agcgaaacag ccccgatgca
     7081 gaattgtatt gtaatatctg gttttgatac ccagcccatt ccaccagtcc gcctgcaaat
     7141 cccgtacagg gtcatacttt ttcgaagcaa atccaaagag ccagtgtccg gagggataag
     7201 tggggatatg agcctgatac accagtgcaa tcgggaagaa ccccttgatg cggctgtgtg
     7261 cccgcttcat ggcgttggaa taggacgtgt agaatgtgct ttcatgctgg ttgaccagta
     7321 tgccgtcctc ggtcaacgcc ttatagcagt tgccgtagaa ttccttggta aacagacctt
     7381 cacccacgcc gaacggatca gtagaatcca caataatcag gtcgtattcg ttttccacct
     7441 tgcggacaaa ccgcaggccg tcctgatagt aaatatgtac ccgcggatcg tccagactgc
     7501 atgctgtaaa cggcagatac tctttcgcca gatgcaccac cagttcgtcg atctccacca
     7561 tgtcgatctg ttcaatggag gggtagcggc agagttcccg caccgtgccg ccgtcgccgg
     7621 caccgatcac cagaaccttt ttgatgtttg ggttggtcgc cataggaatg tgagtaatca
     7681 tctcgtggta gatgtactca tctttttcgg ttaccatcag cagaccgtcc agcgtcataa
     7741 tgcgtccgaa ctccacggaa tcaaacacgt cgattcgttg gaattcgctc tgcacactgg
     7801 caagctgcct gtcaatgcga atggagagcc ggacgttatc cgtgtgattt tcggtatacc
     7861 agatgtccat atcaaaactc ctttcacttt gccttttcca ttaggattcc ttgaccagtt
     7921 ttcctgtcat atgctccggt gtcagtgcaa ggcatagcac attcggacgg gaatgctgca
     7981 attcatgttc cagatacgcc ttgtcgtcag taaacttctg gcagaggtgc gtgcaaattt
     8041 ccagttcctt ttccgcatct ttcaccggct gaatcctgcc gaacactaca acacttttga
     8101 tatgcagcgg ccactgaccg tcctcccgat agccctcgtc atggatacag taggaaacct
     8161 tgtcgcattg ggaaatggca tcgattttgt gacctgtttt cgctccgtgg aaatacagct
     8221 tgccgtctgc ttcgcagtac cagtggttca tgggaatgcc gtaaggatat ccgtcatcgc
     8281 ccagtacaga taagatgcct ctcggctcgt ttttcagaat tgcaatgcag tcctcgtcag
     8341 aaagctgctg cttccatctt cgcatcggtc gaaacatttt tatgcctcct ttttcataga
     8401 aacgttttat tcaacaagct gagatttcgc aatttctaaa ttcacctcag cagcaatcac
     8461 aataatataa aactataaaa catcacgaaa atcacggaca gaacgttgct tttccaattc
     8521 gtcaagtgct tttttcgcag catatttcgc gtattgcagt gcctgttctt ttgtcatgtt
     8581 gattctcctt tgcaaactgg aatttattgg tgtggtttat accaaatatc tatttcttct
     8641 ttttctggaa tttcaaacag agaagaaact ttatttagta atccctcatt aacataaaaa
     8701 cttgatccgc catttccaga tatgacttca ttatttcttt tttcaatatt ttcataccaa
     8761 gtcttatcat caatatcaat ataatgcatt tctaccaaaa ccccattatt ttctccatat
     8821 ctttttattt ctttcctgtt ttctttcgtc caaaatcccc aatctaaaat tacagtgcat
     8881 cctgcattta caatttccac tgcttttttt cttaaatata aattaactct tatagcaaat
     8941 tcatcatagc cgtcaccttg ttgattattt gttaaatcat aggttacttc atctgtagat
     9001 aaaatcacag catgctctt
//
LOCUS       DAWBGR010000099         8925 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2171_length_8925_cov_5.608343, whole genome shotgun sequence.
ACCESSION   DAWBGR010000099 DAWBGR010000000
VERSION     DAWBGR010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8925)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8925)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8925
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2171_length_8925_cov_5.608343"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2023 a         2537 c         2304 g         2061 t
ORIGIN      
        1 aatctgcacg cagattgcgt gcaaagccgt caggcgggct ttatgcagtg tttccataac
       61 tccgcaacag actgcatcaa aggagaagtg aatgatatga agaaacaacc ccaccgctat
      121 atgcggaaaa cagcagcagg catggttgct ttgtccatgc tctgtgccgc agcgattccc
      181 tgcgtactgg ctatgccggc aggagccgca agtgcctccg gtgatctgaa cggggacggc
      241 tctgtcactg ccgcagacgc tgccattttg cagactgctc tgctgggcag ttccaagctg
      301 actgcccggc agtatgccaa cgccgatgtc accggtgacg gtgctgtcaa cggtctggac
      361 ttgtcccgcc tgcggcagat gatcgccact gtaccggtca gcgatgccat tgccattcac
      421 ctgagcgaca gcggcattac cgtagagggc gacaccaagg gtgtcactgc tgtttctgga
      481 aaaaccgtga ccatttccgc ctccggcaac tacaccgtag acggcaccat tacagacgga
      541 cagattctgg tcaatgtggc agatcccact gcggatcctg atgcggtttc cctgtacttg
      601 cagggcgtgg ccatgacatc ttccaccggt actccctgca ttctgggaca gagtgcaggg
      661 aagctgaagc tgacctgctc cggcatcaac accctgactg acacggcggc agctgtcaac
      721 gccgacacct gcggtgtcat ctacggcgac tgtgacatta ccgtcaccaa gaacagcacc
      781 ggcaccctga acatcaccag cagcatgaac acggcgatcc gttccaagga cgacatcaag
      841 ctgaacggcg gcgacatcag catcaacaca gatgtcgatg ccacttctga tgccgatgcc
      901 attcgggcaa ataatacact ggaaatagac ggtgcttcgg tgacggtgac ttcttccgca
      961 gacggtctga aatcctccaa ggaggacgtt tccattctgt ccggcaaagt cagcatcaag
     1021 gcaggcaatg atgccgtgca ggctgccaca gccctgaaca tctccggcgg tacggtgacc
     1081 gccagcggtg accgcggctt tacgctggac gaaaacggcg tgcttgccat taccggcggc
     1141 gatgtgctgg caacggcgac ggactacgca ttcggcatgg attctgccgg tgcagctgtg
     1201 actgtggaca cttctggctg cacgcagggc gtggtacagc tggactatgc ggcagagtgg
     1261 aaaaagagca atgctgtgac tctgaaaaag ggcagcagca ctgtctttga aatgacaccg
     1321 aacaagaagt acaactatgt gcttgcaagc agcagcagcc tttccggttc ggacagctgc
     1381 actctataca ccggcggcac ccagatgaca cacgacggtt ccgataacgg cacctttgcc
     1441 atgaccggca ccctcaccaa gttcaccggc gtacaggaac tcgccggcag cagcgtaaca
     1501 ccgacggacg atactgtggc aacggctctg gtctacaacg gcagcagcgt cacagcgacc
     1561 aacgcttccg gcagtgtcgt ttccaacccc tcgaacctga cgatctccgg agccaatgta
     1621 acagttaccg cttccagcga actctctgtc agcggtgaaa gcaccagcgg acagcttgcc
     1681 gtcaacgtgg ataagaccgc ggaaccggag ggcaaagtgg tgctgaatct ggagggactg
     1741 accttgtcca acgacagcgt tgccccgatc tatgtggagg cgatcggtga cgaagtgcag
     1801 atctctgcca agaacggcac gaccaatacc atttctgacg gcacgtccca cacggacacc
     1861 tatatggaca gcgacggcaa caccgatcct gtcaacgggg cgatcttctc ccgcgatgac
     1921 ctgaagctga agggcaaggg cacgctcatc gtcaacggca acaccgagga cggcatcgtc
     1981 tgcaagaacg acctgaagat ctggaacggc agcattaccg tcaacgccgc agatgacggc
     2041 attcgcggca acgactccgt ccgcatcggc gaccctgatg ccaccgatta cagcactctg
     2101 tctgtgacgg tgaacaccaa taacgacagc accggcggcg acggcatcaa gagcaactcc
     2161 accgaaacgg ataagggcta tgtcaccatc aacggcggta cagtcaacat caattcttat
     2221 gcagacggca ttcaggcaga gcagaccttc accatgaacg gcggcgacct gaatattacc
     2281 acatatcagg gctctgactt ttccggcaat gcttccggcg gctggggcgg cggcatgggc
     2341 ggtgacggca actccagcaa gaccgacatc agtgccaagg gcatcaaagc agtcgggctg
     2401 tatgatgagg ccggcaccac atggcaaagc ggcggcaacc tgatcgtcaa cggcggtacg
     2461 atcaccattg acgcctccga cgacagcctg cactgcggcg gcgatatgca gctgctgggc
     2521 ggcagcatga cccttgccac cgcagacgac ggtgtgcaca gtgaccacgc tctgattatc
     2581 ggcagcaccg gcggtgatga tgacactccc tatgtgaaca tcaccaagtc ctatgagggc
     2641 attgagggtg tggatattac ccagaacagc ggtactgtta tggtaacctc cagcgacgac
     2701 ggctacaatg ccgcaggcgg ttccgacagc agcggcaacc ataacaacgg cggctgggga
     2761 caaggcggct ggggcggccc cggcggcgga aactccagta acggcagtca gaccatgacc
     2821 tttaacggcg gttataccta tgtcaatgca gccggcgacg ggctggactc caacggcaac
     2881 atttccttca acggcggcta tgtctttgtt tcccagaccg gcggcggaaa cggcccgctg
     2941 gactgcggcg acagcaataa ctcgatcacc tattccggcg gcaccgtcat tgcagccggc
     3001 agcagtgata tgttcgaaac acccagcagc tattctttcc tgtcccagac ttctgtttct
     3061 gccggacaga ccattacctt tacagatgcc agcggcaacg tgcttgccac atttaccctg
     3121 cccaatggct ctgcggaaat ggtaatgtcc agcaaggaat ccagtgtgac ctgctatacc
     3181 ggcggcacgc tctccggcac gacatatttc gcaagtcagg acgacacaga ccgctgcggc
     3241 tacggcggaa ccattagcgg tggtacgacc gtttctgcaa gttccggcgg caacggcggc
     3301 tggggcggcg gcggaaacaa ccgcccgttc taaggcaata ccgcaaaaca gtttccggag
     3361 cgtgttcgca taaacgctct gtattttctc tcttgaaata gtgcctgtag aagcaggaat
     3421 gcccattttc ccctcagggc agcattcact gctgccgcag taaaaaagcg gagatccggt
     3481 tttcatcgga tctccgcttt ttgtgtttat tgggtgtgcc ttgacaatgt tcgacagggt
     3541 atgctataat aaagtcctag ggcataccga aaacggtagg cggttcatcc atgctcccat
     3601 gtacaatttt gtacgttggg aggtgatgct atggaacatt acgtaacatg gtcagatttg
     3661 tttgcacttc tgacattcct tgtcgcatac acaggaatca tgtacagtat ttttcatgac
     3721 gatcataaaa agaagtaacc gcctgactct cacataaggc ggttactttt taatcaccaa
     3781 catgggagca accgtctacc ggtatgctct ttctattttt attatacgca tttttttccg
     3841 gtttgtcaag gggcagaccg gcgttttttc agttttgctg tcttgacaaa attcgacagg
     3901 atatgctata ataaagccct agggcatacc gaaaacggtg gacggtttcg tttcctgctc
     3961 ccatgtacaa ttttgtacgt tgggaggtga tgctatggaa cattatgtga cttggtcaga
     4021 gctcttagct ctcttgacac ttttgattgc ttttgcatct ttgctatacg caattttcca
     4081 taacgatcat aaaaagaagt aaccgcccca agctaccaac ttaagcggtt acatctttaa
     4141 tctcttaaaa catgggagca accgtctgcc ggtatgctct ttctattttt attatacgca
     4201 tttttctccg gtttgtcaag gggcagaccg gcgttttttt cggttttgct gtcttgacaa
     4261 aattcggcag actatgctat aataaagccc tagggcatac cgaaaacggt aggcggttaa
     4321 tccacgctcc cagaagggag tgtatgcata tgaaacttga tatgattgtc aagcttgtca
     4381 gcattgtttg tgatgtcttt aaggcatatc ttgcttactt aaactctcaa aacaaacgga
     4441 aataaccgcc ccacttccac atagggcggc tatttctaaa agccattctt aaattgggag
     4501 caaccgtcta ccggtatgct ctttctattt ttattatacg catttttctc cggtttgtca
     4561 agaggcagac cggcgttttt tcggttatgc tgtcttgaca atattcggca gggtgtgcta
     4621 taataaagcc ctagggcata ccgaaaacgg tggacggttt cgtttcctgc tcccatgtac
     4681 aattttgtac gttgggaggt gatgctatgg aacattatgt gacttggtca gagctcttag
     4741 ctctcttgac acttttgatt gcttttgcat ctttgctata cgcaattttc cataacgatc
     4801 ataaaaagaa gtaaccgccc caagctacca acttaagcgg ttacatcttt aatctcttaa
     4861 aacatgggag caaccgtcta ccggtatgct ctttctattt ttattatacg catttttttc
     4921 cggtttgtca aggggcagac cggagttttt tcagtttcac cgtcttatcc cattcgaaac
     4981 gccccgttct gttcctgcgg ctccggtgtc cgcagctgtg cctgacagag atgcttttgc
     5041 ttggtgcgga gcgtttcctg aaaccccagc agttcctcca gcgacatgcg ttccaccgca
     5101 gcagaaaccg catcttgtgc cgcactgccc tccaccagaa accgcagccg cacaatttcc
     5161 tgccgcaggc tgttctccac gcggttcagc agagcgttcc ggttttgcag ctgctgttcc
     5221 agctgtttgc agcggcgttc gccgtcggtt tccatgccga actgcttggt cacccctgcc
     5281 tcccgctgtg ccggcactgc cacaaaactc cactcatagg catctgtcac atcagacagc
     5341 accacatgac agcgtttctc cccgtacagc tgccccacgc ggtgtgcaca aggattcttt
     5401 cggcggtcac tgccgcagac cgagcaggtc tgcgatacgg cagcacagga aatgctcact
     5461 tccttcttga tgccgccgtc aatttcccga atcagatccc gattggcatc ggtgcgtacc
     5521 atgtaggcat gccctttcag aaatgtgtac acttcgccgg cagaggtcac acgctccggc
     5581 tcctgtacca gttccgcagc atagattcgt gcagtctgat tttcaccctt gggatcgtgg
     5641 tcaaaaatgc cggttttccc cacaaacaaa gctttcagct gctccagagc ctccagcgaa
     5701 aagcgttccc cgtcccgatc aatttcattg tcacagagcc gcacatcaaa gaggaatacc
     5761 tcctcctgtg tgagggtacg ccgcgtaaag cggttcagct gttccagctt ggcttccagt
     5821 tccgtgccgg acggattctg ttcctgatta tccatagatc tattcctcct gttctgctgc
     5881 actgttttcc acactgtgga aaaccctccc gcgaaaaaac ttctcccacc cgtacagcgg
     5941 tttccttgat tttccacact gcctgtggaa aactattttt tatgcggcag cctattttcc
     6001 acactgctgt gtggaaaacg tactgtcagc ttgtgtgcaa agccgtcaag cggacttttt
     6061 gcacaatgct tccacaaact tacaggctca gcacatgtac tgcttcactc agcatcacgc
     6121 ggaacgccgc acgaatcgaa accgtgatca cgtccagctg gctgtcaatc agcttgtctg
     6181 tttccagcag cacatcgtct gtggtgatca tctccagagc aaagcggttg tccagtccca
     6241 cgatgtaatc tgcggacatg ccggcacact tttgcagctg ggcaccaaag ggcagcaaaa
     6301 tggtgttggg ctgtgcagaa gccatatcct gcatctgctc cattgccatg atttccgcag
     6361 ccaccgccgg agccgccagc accttggtca tgtcgaagtt gcggaattcg ccgtacagct
     6421 tcgccagatc agcataagtc agcttgccgt ctgtgacaac gtcgatcagg gaaccattgg
     6481 tttccttcat gcacaaaatg ctctgtccca gcagcccgtt ggacagacgc acgcccacgg
     6541 cacggaggat cgcactgaat gcgtccagac gctggcggcg gactgcctca taggaagcgt
     6601 gaatggaacg gccgtacttg tccagtcgca cggtgtttgc tgcttccaga taggaggcag
     6661 tgggcagggc attgccctgt gtggtcttgg tggtataggc ggtagtgtcc gtcagcactg
     6721 ccggctgata ctctccggcg ggagaaatgg tgtgcactgc caccagatca gacagcacag
     6781 actgttccat gccgctgcga atggcacggc ggataaactc cggaaacagc accgcactgt
     6841 ctgtggtgga gaagaacttc tccacgcggt cacagtggga accgctgacg tgaatgtcaa
     6901 aacgcttcag ctgacgctca tacgcgtcca gtccggcaag gggtgtttcg gcatacgcgc
     6961 cagacgggtc catgccctcc agtacctctg aaaagctctt gccagtgatg tgatacatgc
     7021 ccttttccag tttcagatcc tgatacataa aattccttcc ctttctgccg cttgatgcgg
     7081 catgtgtttt tattatatgg aaaccggcag tttctgccgg aaattcccgt ttatgccgga
     7141 taaaactcgt cctgatacgc ttccagcctc gtccgcacac cgttctgatt gccggtcatg
     7201 cggatatggg taatgggagc gttttcaata atgccggagc aggtgatccg gtcgcccaga
     7261 tctgctgcca ctgctccgat gcggtcgaaa tagtaggaaa ccaatccccg tccggcaaat
     7321 ttccgccgaa acaccagagc ctgctgcggg tcgtagagat actgccgatc cagcggcagc
     7381 tgttttgtcc gcaccagttc cagtgctgcg gcgttggggt cgttctcctg aaattttcca
     7441 taggtgccgt cggcatctgc catggaaaac cggctgtaaa tgcgggactg gtctgccgtc
     7501 atgcccatgc ccagcagtgt gtctgcaccc acataaaaat tccggtatgt ttccggcagg
     7561 tgcatgcgaa tttcgttggc atggtacaca aagggatacc gcccgcaaag cttgtaggtc
     7621 aggtttgcta cgccgtccca catgctggtg ccctctttga caaagagata gttgctggta
     7681 tccgtgtcag attcccacgt gatctccttg ggaaatgtca cgaaatcctg catcagcttg
     7741 tccagcgaca tagcagtgtg cagccccggc tccagctgat tttgcagcag cagtgccgtc
     7801 agcccgtggg aggaaaaccg cacataggag gtgccgtttt cctgcaccag ccggaacctt
     7861 tccactgtcc cgaagtgcag ttccgtgccg tccagcaata gctgtacccg atagatgccg
     7921 gcgaactgct gtatgctgaa attcccgtac accgtcgccg tcagctggct gtagggcgtg
     7981 taggcttcct tgtccagcgt aaaggcggca atgccgtttg caagatacgc ctttccggtt
     8041 ctggtaatta acaccacctg tatcatgtac tgcctccttc cgtttccgcc gtcacacggc
     8101 tcagggcgtt gggagcgatc agcgtcagct gacagggcag cacgccctgt gccgccgttt
     8161 ccgaaatcgt atacgctgcc aagctggtgc cgaaaaaccg cagatcccgc agatcaaagg
     8221 cgaacagtgt ccgttcccgt atggcggaat ccaacgccag aaccacctct gcggggctgt
     8281 ctgcgaagca gaatttgccg tccagcacca actgtctggc acggcagctg gtattggtca
     8341 cataggctgc tccctgtaca ccgccctgct gggcgtactg tctggtaccg gaaagcttcc
     8401 agccggcagc gtaaaaggtg agattgccca gacgaacggg aaactcccgc ggggaaacca
     8461 cctgaaatgt tccattcatg gtgcaagcac ctcctctttg gcaatttgaa agacaccgga
     8521 gagagtaaag ctgccctcca gtgcaatttt ccggaactgt ttccactccg tcggtgctcc
     8581 ggtgcgaatc ttctgcacgg tgcagccggc ggacagcata ccggaaacca ctgtctgcca
     8641 atagcggtca tacagcactt gtgcatccgt ttccggagtt gtcaacagcc gcacttgagc
     8701 agcggcggaa aatggaaaat accagtaagt ttcgtcggaa aacgcctgct ccgtttccag
     8761 tgcagtgatt cccacggtga ggaaagattc ctctcgtttg gcaaagggtg cgggatcata
     8821 cgcggcataa accgcccagt tgccctttac ccgaagtgcg gctttcagcc gttccaataa
     8881 taattccagc agcatttatt tacattcctt tctctttgcg gcggg
//
LOCUS       DAWBGR010000100         8826 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2194_length_8826_cov_5.079922, whole genome shotgun sequence.
ACCESSION   DAWBGR010000100 DAWBGR010000000
VERSION     DAWBGR010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8826)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8826)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8826
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2194_length_8826_cov_5.079922"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2000 a         2216 c         2432 g         2178 t
ORIGIN      
        1 gacgggaaat ttgcaagcag atggcgtgca aagtcgccag acgctctttg tgcgatattg
       61 ccaaaggcct atttacaagc acaaaactgt cgaaagacga ggaggctcta tatgagaaaa
      121 gtacagttta cggaaaccgt tttgcgtgat gcaaaccaat ctctcatggc aacccgtctg
      181 ccttatgcag actatgcaga cattctgcct gcaatggaca aggcagggta ttattccatt
      241 gagtgctggg gcggtgcgac ctttgactct tgtttgcggt atctgaacga agacccgtgg
      301 gaacggctgc ggaatctccg gaagaacctg cccaacaaca agctgcagat gctgctgcgt
      361 ggacagaacc tgttgggata taagcactat catgatgacg ttgtggaaaa gttcatcgaa
      421 ctgtccatca agaacggtat cgacattatc cggatcttcg atgcactcaa tgacttccgc
      481 aactgcgaaa ccgcgttgaa tgcgaccatg aagtatgcca ccaagaacac cattgcctct
      541 ggctgtatct gctataccca gagtccggtg cacaccgtcg aaaagtatgt ggaaatgtgc
      601 aaggagctca agcgtatggg attccagacc atctgcctga aggatatggc tggtaccatg
      661 actccgtatg aggcagaaca cctcatcaag ggcatcaagg acgcagtcgg cgacatgccg
      721 ctgatcctcc acacccacag caccaccggt atggcatata tgactttgac caaggcaatc
      781 gagtccggca tcgatgtgat cgacaccgcg acttcctgct tctccggcgg tacttcgcag
      841 ccgtctacag aaaccatgtt ctatgcctgc cagcagtacg gcattgaaac cggtctggac
      901 gaaaagatcc tgaacgaagt caacgactac ttcaagccga tcaagcagaa gtatatcgac
      961 aacggtcaga tcaaccccaa gagcctcggc accgatgcac aggctctggt atacaaggta
     1021 ccgggcggca tgctgtccaa catgatcgca aacctcaccg atatgaaggc aatggacaag
     1081 tttgatgctg cactggcaga gatcccgcag gttcgtgctg atatgggtta tccgccgctg
     1141 gtaacaccgc tgtcccagat ggtgggcaat caggcagtga ccaacgttct ggtaggcgaa
     1201 cgctatcgcc agatctccaa ggagatccag aactatatca agggcgaata tggtatcgct
     1261 ccggctccga tcagcgaaga actccagaaa aaggtactgg gcgagggcgg caagccggtt
     1321 gactgccgta tcgaggacca gaagcgtacc ggacaggaat tcgaagatgc caagaaggca
     1381 ctgggcgatc tggcacgttc ggaagaagat attatgagct atatctgcta tccggatcag
     1441 accatgaaat tcctggaagc ccgcaaggca caggaggaaa acgaagccgt ttataccatt
     1501 gaggcgatgt aaggaggaac agctatgcca ttaaatgtga tgagtgcatc agacatgggc
     1561 attggcgaag caggtctgac cgcgattttc ggttatgttg tggtattcgt cggactgctc
     1621 ctgttgatgg tcgtactgta tatcatgggt gctgtgttca agtcgaaggc aaagaaagcg
     1681 gaagcactga aggctgcgga acaggctgct gctccggcag aagcaccggc agctgagccg
     1741 gaaaaacccc ttgctcccgg ttctgccggt cacgtcaagc ggtttgacgt tccggataag
     1801 gaagccgcta tgatcatggc aattgttgcc gatcagctga aaaagccctt gaatgaactg
     1861 cacttcgttt ccattaagga ggtcaaagag gatgaagtat aaagttacac tgaaccagaa
     1921 agtttatgaa gtagaagtag aagcaggaaa ggctgtgctg ctggacgaat atgaggcttg
     1981 tgcaccggct ccggctgctg ctcctgccgc agaagctact ccggctgccg ctgctgctcc
     2041 tgcaccggct ccggcggcaa aggtagacct gaaggccggc gaaccggtaa aatctccgat
     2101 gcccggcaac attatccgtg tcgaggtcaa ggagggcgat cagatcaagg ctggacagct
     2161 gctggtcgtt ctggaagcca tgaaaatgga aaacgagatt ctggcacctc acgatggtac
     2221 ggttgcacag gtcattgttt ctaagggctc tacggtagaa accgattctc cgctgatcgt
     2281 gctggcgtaa aggaggggta ttcatgaaca tgcttgaaac ccttcagaat cttctgaacg
     2341 attccggttt cgtcagcttc tttgctgacg gcggctggaa aaaccttgtg atgatcgcgg
     2401 tatcctgcct gctgctgtat ctgggcatca aaaagcagtt cgaaccgctt ctactggtag
     2461 gtatcgcatt cggctgcctg ctggtcaatg tatccaactt cttcatagac ggtgcaagct
     2521 cgtccaatgc cctgtaccac cccgaattgt ggagtgcgtt tctggaagaa ggcggagaat
     2581 actaccacag ctatgggcac atcatggcga acggcggact gctggatatt ttgtatatcg
     2641 gcgtcaagtc cggtctgtat ccgtccctga tcttcctggg catcggtgct atgactgact
     2701 ttggcccgct gctgtccagc ccgaagaact tgttgctggg tgcagctgca cagatgggtg
     2761 ttttcctggc gttctttggt gcggtttgcc tgggctttga cggcagtcag gcagcatcca
     2821 tcggtatcat cggcggtgca gacggcccga cagcgatctt cctgaccagt aagctggctc
     2881 cggaactcct gggaccgatc gctatcgctg catactctta tatggcactg atcccgatga
     2941 tccagccgcc tctgatgaag ctgctgacca ctgaaaagga acggaaagtc aagatggaaa
     3001 tgactcgtca ggtttccaag accgagaaga tcattttccc gatcgtcgtt gctctggtgg
     3061 ttatcctgct gctgccgtcc accgctccgc tgatcggctg cctgatgctg gggaacctgt
     3121 ggcgggaatg cggcgtgaca gagcgtctgt ctgataccgt acagaacgcc ctgatgaaca
     3181 ttgttaccgt attcctcggt atctccgttg gtgcaactac ggttgcttct tccttcctga
     3241 ccctggatac cctgaagatc gtggttctgg gtctgctggc attctgcttc tctacagtcg
     3301 gcggtctgct gggcggcaag gtgatgtata tcgtcagcgg cggtaaggtc aatccgctga
     3361 tcggttctgc cggtgtatcc gcagtgccga tggctgcccg tgtttcccag aaggttggtc
     3421 agaaggcaaa cccgtccaac ttcctgctga tgcacgccat gggtccgaat gtagccggcg
     3481 ttatcggctc tgcaattgca gccggtttcc tgcttgctgt attcaagtaa gtttctcatc
     3541 tcatatccgt atataacaaa cgcccgatac aggcacgctg tggcttgtat cgggcgtttc
     3601 tctatctcgc agggaaaggg tactggtgtt ccgtaaaatt ggttctgaca ggctttctgc
     3661 ggcttctgaa cggtcggtgt gttggtggaa gtctgactgc ttacggggaa cagctgcttt
     3721 cagacaaaca aaaatccccg cagacaatgg actgcgagga acaatgcgat tatttcattg
     3781 ttatacatac gaagatttgt agctactcct gaaacaggag cacgtcaccc ctgtggtgac
     3841 ggctgatctc aagaagatgt attgtgaaat cgaaaaaata agttctagta aatcagttta
     3901 tacaggttgg ggaaaagtgc aaggtattcg ctcagcttgg tacgcttgct ggaacccgga
     3961 tcgttgatga taaagttttc tgcggagaat gtggtgccct tggaaccggt ataaccggta
     4021 accaatacgt aatgctggct gccgttggac ttctttgcac cgatgacaac cggcttgttg
     4081 ctcaacagct tgtcatagat cttctgcatt acgctctgtg aaatggtgcc gctggcattg
     4141 tctacggtgt agcccagctt tgtgcaggat gaccaaatca ggttgtcact gctgtaagtc
     4201 agcttggata ccattttgtc cggtgtggtg ttggtgttgg tctggtaaga atacttcatt
     4261 gccagagctg ttgtcacaca gccgaccttg ccaatcgtgg aataattgat gaccttgttg
     4321 ctccatttgg agtcgaactg cttgtagtct accacattca gctgtaccgg tgcatgtccg
     4381 gtttcggtga gaacaccggt cgtcggatca aaatagctgg aggaatctcc aatggtgatc
     4441 cagccggttc gcattgcaaa ggtggacgga tcaaaataat aggttttgtc gccgataacg
     4501 gtcacaccgg aagccgctgt accgtcagcg ttcagataaa tcttggaatt gtccttggta
     4561 gtccagccag tcagtgcctt gccgtttgtc ggattgaagc aatacagctt gccatttaca
     4621 gtctgccagt ctgtcagcag aatgccattt gcattgaacc cgtagatccc gccgtcaatg
     4681 gtctggattc cggttgctgc accggaggtg gtgaggtaca tggtaccgtt ttctgtttcc
     4741 agccagcctg tgcggagata tgcttcatcc ggatcgaagt aatactgtac accgttgacg
     4801 gtgatccagc cggtctgcaa agcaccagtc tgttcgttga agtagtaata cttatcgttg
     4861 atctgctgaa tgccggtctg tacagcacca ttggcatcgg tatagtaggt gatgccgtct
     4921 atggcacaaa caccagtcaa agcgtagcct tgtgcatcga agcaatgatt cttgccgtcg
     4981 atgctgacca ttcccttggc gagtacgccg tcggcatctg cccacatctt gccggaatcc
     5041 gtatcgatcc agccggtctt cagcttcttg tcgtcaccga agtaatactc cttgccgtcg
     5101 atcgtatatc ttccggttac ggctcccttg tctgctgtca ggtagtaggt accgttttcg
     5161 gtgactgccc agccggttgc cgttgaagca tctgtgttgt agaagtacag cgaaccgtct
     5221 gcataagaaa cccagccggt gactgcacag ccgtattcgt caaaggtgta cgggataccg
     5281 tcaatggtct ggacaccagt cagcttgccg gcagttttat cgatatagta catcgcattc
     5341 tgataatcca tccagccgaa taccggctgt ccgacctctg cactataata ataccgcttg
     5401 ccgtctacgg tctgccagcc agttcggagt gcaccgtcag aagcaaataa ataagtatag
     5461 ccgtcgctca gtgtggtttc gtcctgtgca cagctgccgt cgctgtagta ataataaagg
     5521 ctgtcatcgt cagatacata ccagccgctt tcagtgatct gatctgcttc gcagcttcct
     5581 gtcaggacac ggttgccgct gtctgttaca ataaagctgt tcagatgcag gatgctgtcc
     5641 tgtatatcat actcttctgc tgctgcggaa attgtgccga tcatcagaga agcgagcagt
     5701 ccgccggctg cagcaaagag aagtttgcgc atcggttgcg ccattggttt cacgtccttt
     5761 cgtgtcaggg aactgccgcc tgtaaggcgg agtttccttt tgggcgatcg ctgtttccat
     5821 tttgcaatcg ctgtcgtctg cgttcgtatg caatcgacaa aattttcctg ccgattgtaa
     5881 tcgatttgtc atgaatccac tatttattat accgatgttt tggggtgctg tcaatcgatt
     5941 tgccgtcttg ttcgaaaaaa ctgtcgaatt ttcggcacac tgtacaaaaa gcatctgctg
     6001 cactcgccca ttctgcacaa ttttttcgac gattttattc ctgatattgg caattacaaa
     6061 atgaaatgtt ggtaacaaaa cggaatcagg agcctgccgg cggcgttcag cgggggagaa
     6121 cgacggtggg cggcgtgctg attgcacggt aatattccgg ccttctgtcg cggaatacgc
     6181 cccagctcat acgcatttct tccagctcgt ccaagtcgta tgtctgcata gcaatgccgg
     6241 cttcgtcccg tcccagctgc tgtaaaactg caccggtgcc gtcggtcagg aacgaggtgc
     6301 cgtagaacga cagagaggag gtctgctgct ggttctcctc atcgggggtg atgtgctctg
     6361 tgccgatgcg gttggcagcg atcaccggca tcaggtttgc ggcggcatgt ccctgcatgc
     6421 aacgctgcca gtggggagca ctgtccacct ccagaatcgg ctccgagccg attgccgtgg
     6481 ggtagaacag cagttctgca ccggcgagag ccatgcagcg ggcagcctcc ggaaaccact
     6541 gatcccagca gatgcccacg ccgattctgg catagcgggt gttccagact ttgaagccgg
     6601 tgtcgccggg ggtgaagtag aatttttcct gatagaaatg gtcgtccgga atgtgtgtct
     6661 tccggtagat acccagcacc gagccgtcag cgtcaatgat cgcaatggaa ttgtacaggg
     6721 cagtgccggc tttttcgtag aagctgacgg ggagaactac ctgcaattcc gcagcgactt
     6781 tccgcagctg tgcaatggca ggattttccg ccggcgtggt tgccagttca taggaggcgt
     6841 accgccgttc ctgacagaag taaggggttt cgaacagttc cggtagcaga atgatctgtg
     6901 ccccgtttcc agctgcctgt cgtaccatcg cttcggcagt gcggcggttt tcggcggcat
     6961 cgggagagca tgccatttga atggctgcaa cagtgacttt acgcataaat agaactcctt
     7021 cctaagggaa tctgctgtgt gatacagtgg atattgccgc cgcccagcaa aatttctctg
     7081 gcggcaatgg gaacgatcgt ccggttcggg aactgggcag acaaaatttc acaggcggtc
     7141 ttgtccatgg ggtcgccgaa ctgcggaacc agaacgctgt cgttggcaat gtaaaagttg
     7201 acataggagg cagccagccg ttcgcctgcg gttctggtgg gttcgccgtc ccagaggtct
     7261 aactcgctgc attcctcctg tgtaatgcag accggcttct gcggaatggg cagcagtgtc
     7321 acttccagtt ttcttccctt ggcatcggtc tgctgtgcca gatagtcggc acaggctttt
     7381 gaaagggcat actgcgggtc gttctggttg tccgtccacg caagcactac ttttccggcg
     7441 gcagtgaacg cacagacgtt gtccacatgc tcgttggttt cgtcgccgta aatgccgcgg
     7501 ggcagccaca ggactttttg ggcacccagc atggagcaga gcttttcctc gatctccgcc
     7561 ttggtcatgt ccggatttct gccggcactg cacaggcagc tttctgtggt gagaattgtt
     7621 ccctcgccgt cgctgtgaat gctgccgcct tccagaatga aatcacggct gtcatagcag
     7681 tcggcacggt acaggtcgca gacttttctt gccgcatggt tgtccttgtc ccacggaaaa
     7741 tacagcccgt ccaccagacc gccccacgca ttgaagcccc agtcgatgcc gcggacagtg
     7801 ccctcggcat tgcggacgaa agtggggcag acatctcttg cccaggagtc gtcggtttcc
     7861 atttccacaa ggcggacttc cggcggcagc attgcccgtg cggtttcgta ctgttccgga
     7921 gccaccagca tggtgacttc ttcggaacgg gcaatggctg cggcaactgc cgcaaaggct
     7981 tttcgtgccg gccagccgcc gcttcgccag gaatctcccc gtgtaggcca gatcagcaga
     8041 cagccggcgt gccggtcgaa ctccgccggc atgcagaacc cgtctgtgcg gggtgtcgta
     8101 tgtaaaatgt gcatgatagg attcctttct ttttcaagca gtttttccac aggctgtttg
     8161 atttttgtgg aaagctgtcc tgtaatgact gttttcatta tagcatattt ttttggggaa
     8221 tgcaatcgaa ttctatgggc ggtttttcac gagattgtgg aattgctgta gagggcgtgg
     8281 cgtttccaag gaaacgcaga ccttttcggc agaattccgc gtatgctgtg gaaaatccgg
     8341 tgtataatct gccgcagatg tggaatcgta aagacaggga aacaccgcat aaagcttgtg
     8401 cgtaagatgt gttgtcagat ttttcggcag attgcataga aattccgcag gcatacttga
     8461 tgtatggcaa ggggtttcta ggcaatatga cggaaaatct gcaagcagat tgcgtgcaaa
     8521 gccatcaggc gggctttatg cagtgtttcc acaataccgc acgaagcgat cctgcggtga
     8581 aaaatgcgga ggtgaagatc agatgaccag taaagaattg ctgtatgtag aggacgcact
     8641 gggacatgag aagtattttc agaccaagtg catggaggct gccgcacaga tgcaggacga
     8701 agacctgaaa cagtgtgtga cgcaaatggc ggagaagcac cgccagctgt tccggaattt
     8761 ttacggactg ctttaaggaa acactgaata aagcttgtgc gtaagatgcg tagtcagatt
     8821 tttcgg
//
LOCUS       DAWBGR010000101         8798 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2203_length_8798_cov_3.491365, whole genome shotgun sequence.
ACCESSION   DAWBGR010000101 DAWBGR010000000
VERSION     DAWBGR010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8798)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2203_length_8798_cov_3.491365"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2633 a         1815 c         1975 g         2375 t
ORIGIN      
        1 gcagcattgg cacttgttgt tgctaccatg acagcaaatt caacttgtgc atggttggct
       61 tatcagccgg aagagccgga agaagtgaag atgctgaaaa aggattgatg aggcactgta
      121 tttttagata tgccgataaa ttaatccact tttatattga ttttaaaaga aataattttt
      181 caagatgata accactgtac aggacaacaa aagcttttgg atttctatct tactaaaatt
      241 tttctttgaa gaataaattc gaatatctgt tgctgatttc aataagaatt cgcctgactg
      301 aatgaaagga ggtgaaatca aaatgaccaa acttaaaaaa gtggtagccg gcattcttgc
      361 agcggcagct atggcaacga gcgttgttgg tatgagtgca agtgcatata gtcctactgt
      421 ttccagaact gttggtagcg ttacaggaac actttattct gatacaacgt atggatacgg
      481 tacaacagct tgcagtaatc aaacgtgtta tgtcaaagta aaacacggtg gaacaacctc
      541 aggttgggca aactcatatg gctatgcaag ttgcaaaaac tctgccacta atggaacaac
      601 gaatgcacaa tcatggcaca gaacagcgtc aacatcatca ttcagtcttt atttctaaaa
      661 agcctgttta ttgacttaat ccatttttaa tcaaatgagg ggattgtatt tcacttgatg
      721 tacactcccc tttttgagta taatagacat tttgtttaag aaaatcgtaa ctattcagac
      781 ccccgccata gccgtgactg aatttcgtat acatgcataa tagaaattta cctttcagac
      841 cgatagaaac cgttcaagtg aagtgaaaac aaaatctcga tctattattg gctgtaagcc
      901 cctaaaaatc agcacttgaa aatctgcgtt gatcgcaatt cgtcaatatg ccgacaccga
      961 ttattctgtg catttgcgat atgtctttaa tcgtgatata atgtagatag acattatcat
     1021 ggaggtgagc atatgccgga ttataaggat cagattattg agaatcttca aaaagaggta
     1081 gaggaattaa agcgcattgt cgagatgcag aacgatatta tccgtgacct gagagaacaa
     1141 ctccgcaaga attccagcaa cagctaaaaa cctccgtcat ctgatgggct taagaaaaag
     1201 cctgtcaaca agaatagaag tttgcgtgag aaaagcggga agaaacaagg cggtcagaaa
     1261 ggtcattctg gcacacatct tgctgtgctt tcagagcctg gccatgtcat taatcatctg
     1321 cacgctgact gccagcactg cccgaagcgt gagatttgca tgatgaattc tgaggtgaaa
     1381 gaaattcgcc acgaaattga tatgattgct caggttgatg tgaccgaaca tgagcttatt
     1441 acaatacccg tatgcccgat ttgcggtgaa gcaaaaacgg gttctttccc tgcggaaatt
     1501 aaggcggtcg ttcagtacgg acaaaacctt gaagcactcg ctgttgcttt gaacaccatt
     1561 ggcgcagtca gctttaatcg tactcacgat atattaagcg gcgtgtttaa cgttccgatt
     1621 tctgtcggga cgataaagaa catggtttct cgttgtgctg ctaaagtcga ggaggctaat
     1681 actgtctcgg ctgagaagct gaaatcagca caccacaagc acggtgacga aactggttgc
     1741 cgcgctgacg gcaaaacgcg atggacgcat tgtctttcaa acgaactcta cacagttctg
     1801 tttcttcacg acaaaagagg gcatattgct atggaggaaa tgggtatcat tcagtatagc
     1861 ggcggcactc ttgtccatga ttgctgggct ccttattggt gctttacgaa tgttaagcac
     1921 cagctttgcg gtgcccattt gctccgagag ctgaagggaa tagtggagaa tcatccaaaa
     1981 caaacatggg ctaaaaaatt cacaactcta ctgctgaatc ttaagcgcgc aaaggaaaag
     2041 gcgattgcaa aaggtaaaac cgctctacat cgaaatacct tgaagcggta ttccaacctc
     2101 tatgatgaga tcatgagagc agcttatgag gagaatccgc ttccggaacg aaagccggga
     2161 cagagaggaa gaaccaagcg cggaaaggtg ctgtgcctta tagatcggct ttctgcacac
     2221 aagggagagg tctgcctttt tgcacatgac ttcgatgtgc cttttgacaa caatcaagct
     2281 gaaagagaca ttcgtaacat taaggtgaaa accaaagtgt ccggctgttt cagatcagtt
     2341 gacggggcaa aagaatacct gaaaatcatg tcatatgttt ccacagcaat caaacacggc
     2401 tttaccagtt ttgatgcgat tcgtcgtgct gttatgggca attctatgtc tatctttgct
     2461 caggactctg aatagttaca gaaaatcata atttagaggt gcaaaatatg agtagaattc
     2521 ataagctaat tgttcctatc atgctgacaa tgaatctatg tgcctgtgga agcgaaagca
     2581 tacaaaaggg gagcaacatt caaaacttcg agcaaaatca ctatatcaca catgcgaata
     2641 cttatcagaa tggcgtaatc tataccggtt ctgagggcag ttccacagca gcttactttt
     2701 tagactatga gagtatgcaa gctgttccac tttgtaataa gccaaattgt gctcacaagg
     2761 acacctcgtg cttggcattt cagtgtgttc aggatagcat catgccattt gtttatcaag
     2821 gtatggtgta ttggtttcat acggattcta aaatcacgga ttccgatgat ggaagaagta
     2881 cagcatatca atttcaaact acatgctact gtgggaatat gactacgggc gaagtagaaa
     2941 gcttcgtgga aattccggat gtcagcatga aggatgccat tgaatttgtc atatccaatc
     3001 acacactcta catcatcggc tgtgattgtg catatcagga agaggatgga tcgtggacgg
     3061 aaatgtctcg aatcggagac cagtatcttt atgcgatcaa tctggataca gcggatgtca
     3121 agaattacgg tctcatcaat gatgcaccaa ctgcatccta caattggaca ctgaatggtg
     3181 caatgtttgc agatgtagaa ttgacaggta tctatcaaaa taaactttat ctgtcttatc
     3241 gctatgtaga tgatcccaat gatattattg attatgtcaa caccggagac ccggatagtc
     3301 cggattggtc ggatgccgga acagcaatcc cctggcatcg ggtgaacaaa tgcctcaatt
     3361 tagaaacgga taccatagaa ccaagcgaat atcccgcagc agacttgatt caagacgaca
     3421 catatattta ttatatggat gggcaatacc acgtaatgga tgcagatgga acagagacag
     3481 tgagtgcaga tattcctgca aataattctc gtaatctaac ttttgtcaat ggaatctttt
     3541 gggatggtcc gcaaagcacc tgttttcaac cgaaaaccgg agaaacacat gtcttatccg
     3601 aaacatattc cgatacaaat cttaccgtaa ttgcctatta caaagaaaga tacattatta
     3661 actatacaga tgaaaacaac ctcattcatt ttgaagctgt tccggaatcg gatttgattg
     3721 gaggcatagt agaatgacac tggttcttcg tcattgcaac aaacactatg gcaagaagca
     3781 cgcccttaaa gatttcacat tttcctttga aacgggtgtt tatggattgc ttggtcccaa
     3841 cggtgcggga aaatccacgc taatgaactt aatcacggac aacttaaagc cagacaaaga
     3901 cggcggcgaa atactctggg atggacagcc gattcggaaa cttggaaaaa gctatcgtgc
     3961 ttgcttgggt tttatgccgc agcagcagga attatacgcc aatatgacag cacgagattt
     4021 tctggggtat ctgtcggcac tcaagggcat tcctcggaaa gaggcgaaag accagattgc
     4081 agatgttttg gaacaggtag aactttctga gcaggcagca caaaaaatcg gcggcttttc
     4141 cggcggcatg aagcagcggc ttctgattgc acaggcattg cttggaaatc cgtctctgtt
     4201 aattttagat gaaccaactg ccggattaga tccaaaacag cgtgtgatta ttcgcaatct
     4261 gactgatcgt ttgggacaag agaaaatcat tctcatttct acgcacatca tttccgacat
     4321 tgaaaccatt gcagaccaaa ttctgcttct gcggaaggga acgcttttga cgcacggaac
     4381 agtttctgaa ctgatagagc aggtacagac cggcgaaaag acgctggaga atctctatct
     4441 gcaatactat ggaggtgagg caaatgctgc gggcggagct taaaaaatgc ctatcctttc
     4501 gagtcctttg gattctgctc ttttgtctgc tctgcatcaa tggctattcc caaatcacaa
     4561 atgcttacga ccggtattac acgccttcgg aatataacgc tttatacgag cagttaaaca
     4621 gaatgtcttt acaggaagca aacgcatttg tcagtgccaa aatcgagtct gcttctccgg
     4681 actcggatgc agactacaac ggctatctat actatgacac attggaaatt atcgaggggc
     4741 tgcaaaatta tccggcatat ttggagagca ttcagaccaa tgcagagaat atgacagcag
     4801 tttccatttg gggcgggaca gacacgtttt cctatcggaa cattcaaaaa acgccgtccg
     4861 cctatactgc ccttgctgga accacactgg ttttggatac ctctcttggc atagaggatt
     4921 tgctgtccag tcccttgacg gatttcttgg cggtgctgtt gctattcttt tgcgtctgcc
     4981 gtattttttt acgggatcgg gaacaaggca ttttgccgct gctctatgcg acgcccaacg
     5041 gaagacttcg actgatgagc tgtaaactgg gaatagcagt gctctgtgcc atcggattgg
     5101 tactgctttt ttatggcgaa attgcactga tttcctgtaa cttgtacgga cttggtgacc
     5161 tttcccgtcc aattcagtgc atctatggct atgaaacctg caaccttccg gtttctgtcg
     5221 gaacttacat tagcatctat ctgctgttca aaatggctgc ctatgcggta tttgccgtgc
     5281 tattcggctt tttctgtacg attgccaaaa ataatctgat ggtatacggt gcatccattg
     5341 gcgttgttgg aatatcctac ttactctatg cgaaaatttc tgttttgtca ccgattagcc
     5401 tattgcattt ctggaatccc atccagtttt tacaaggcaa agaaattctc ggaacctata
     5461 ccaatgtcaa ctgtttcggc tatccgattt ctctgaagat ttccgcttgc gtagtcatag
     5521 gactatggct ctttcttttt ataggcagta gcttcatcgt gtttacacac gccgggaatt
     5581 tgcaatatcg cagtttttcc gtccgacata aactgcatat tcggtttcgt gtccatcaaa
     5641 aattttggta cacttgctat cggattttga ttttgcaaaa gggcttgctt gtagtatttc
     5701 tgttgctttt cctatcggtt ggtttggcaa aaacatatac tcgaacttac agcaatgagg
     5761 atatttacta cgaaaatttt tgtaacgaat atgccggagt tgtaacagaa aaaaccgaac
     5821 aatttctcac ggaaaagcga gctttctatc gtgaaatcga agcacagatc gcagagctgg
     5881 aacagtcgga atcgccgaat tcctatcaga ttggacaatt gaccgcacaa ctgaatgaca
     5941 aggcagcttt tgaaaaattt gcacagcgag tagaacagat tcccgattct gcggagattt
     6001 tctacgacac cggatatgtt cgctatttcg cactggatgg caaccgggag ggcatggtac
     6061 aagtgctgct tttaacggct gcactgacac ttttattgtg ccccactaca tctatggatt
     6121 gtaaaaccaa tctaaagcag atttatcacg ccacgaaaat agggagattt ggataccttc
     6181 ggcagatgtt tcgttttgcg attctgaacg gcatactatt ttccgctgtg ctgatattgc
     6241 cctacttgct gcaaattttg aatcattacg gaatgcaggg ctggaacaca ccgcttgccg
     6301 gaatcgaagc atacagtact tgtcctgctc gcatttccgt tggtgcagcg gcaattggtg
     6361 tcatgggaat ccgcacaatt ggagcagctc ttaccggatg cagcatcaca tggattgcct
     6421 ctcattgcaa gagccttgtc acggcgtatt gcatcaatgg tgtccttttc gttcttccgg
     6481 cgggattgtg cctgttgggg ctggatatgt ttcgctacgt tgggctaacg ccgatgttgt
     6541 atgggataat ttagagtttt gaaatggatt tctttccatt gcggagcaac ttcggctgct
     6601 ccgcaatttt atgtgtgttc aatggaacat atttctaaaa agtgaaaatt tcaaatccac
     6661 ctgacgaaaa aaatcgaatc agaccatgta caaagttaaa agggtttact gacgcaggta
     6721 acgctgagcg atggtaccgc aaatggcgaa aagaatatca ccaagtattt ctacaacaac
     6781 ggcggtattc aaaccaagat ggaaaccggt ctgaacagtg caaatgattc ggactacatg
     6841 actaccgatt acgcatatga tgcgtgggga catctggtaa ataccactga cagcacgggc
     6901 tacaattccg gtacgactac ctatgactta aacggaaatg ttttgactgt tacggatgca
     6961 aacggcaatg taacaaccaa tacctatgac gcactgaatc gtgttttagc agtcaatacc
     7021 gtttgtgatg acagcacaaa gaacgtttct aaatcctata cttacaacaa tatgggatgg
     7081 atgaccaaca agtccagcaa cggtacagaa acttccattg agtacgatgc tctgggcaga
     7141 gtaactaaag agtggggtcc gaatttcaag ggctacttct atgaaggcgt ttcgaattat
     7201 gtagaaaagc aactggtcgg ttatgcacat ctgttgctgt attctgttac aaactatgaa
     7261 tacgatgcag aaatgcgaat cgtaaaagtt ctggagagtg gcagcgaaac tgcttcctac
     7321 acctacgatg ccaacggaaa caagacaagc gaaacgcagg cgaacggcgt agtttccact
     7381 tacacttata acagtgcaaa caaaatcacg aaccacatca cgaaatccgg caattctacc
     7441 atatctgagt acgaatattc ctattatctt gatggttctg acgctttcaa agtccgcaag
     7501 gaaaagtggt ataatagaga caacatccta cgagtacgat gggctgaagc ggctgacgga
     7561 agaagcagtg acgaccggaa gttcgacaga cacctattct tacgagtatg acgattacgg
     7621 caaccgttct aagatgacgg caactagctc ggaaagttat gtaactgatt acagctacag
     7681 cgatttcaac ggaaattata ctgctttact tcagaaagaa accaaaaccg tagatggcac
     7741 ttccgaagaa attgttgcaa atggcttggc aacgagtccg gcagacttgc tgacaacgga
     7801 taattctgac gtagaacaga cagtttacac ctacgatgcc aacggtaatc agattacaaa
     7861 gactgccgat ggcaagacgg aaaccaacac ttacgatggc ttgaatcagt tgattggctt
     7921 cacagatggt gaaacgactg caagctatac ctatgatgtg gatggcttgc gactgagcaa
     7981 aactgtcgat ggtaaaacga caaaacacgt ctgggacggc aatcaacagc ttgttgcaga
     8041 tgtgttggat agcgagcttt atagtgcaaa ttgctatctg cgaggcacaa atctggtagc
     8101 gacttatagc tatcagaacg gcgaaaagtc cgggtatacc tactatacgc agaacgcaca
     8161 tggcgatgtg gtgaatctaa cggattcgac aggtgaaatt accaagtcct atacctatga
     8221 tgcgtttggt gtggagaaga atatcgataa ctccgatacg aatgcgttcc ggtattgcgg
     8281 tgaatattac gactctgaaa cgggtacgat ttatttaaga gctaggtatt atgatccgag
     8341 tatgggtagg tttatctcaa gagattctta tgcaggtaag aattctgatc cactgagctt
     8401 gaatcggtat acgtattgcc ataataatcc aatattttat gccgatccca aaggacattc
     8461 tgcttggaca aaatttcaag aggcggcatt tgcggttgag catccgttta tagcatcaaa
     8521 aattggaacg gcaaaaccgg atgatgcaaa cagcaatact atatcttcaa gagcagctcg
     8581 ctttgcaatc aattctaaag tatcatataa ttacaaaaaa ggtcaagaaa atgaaggcgg
     8641 tcaaaggaac gctcttagac atgcaatctg gtctacaaca ataactagat attacggtaa
     8701 agaaataatg aagcaagttg gttattctca cgaaaacctt tctgaaatgc tgaaaataac
     8761 atccgatcct acgaaatggt atttttcaga tatgcaca
//
LOCUS       DAWBGR010000102         8628 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2232_length_8628_cov_3.128893, whole genome shotgun sequence.
ACCESSION   DAWBGR010000102 DAWBGR010000000
VERSION     DAWBGR010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8628)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8628
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2232_length_8628_cov_3.128893"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    411..420
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    711..720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2332..2341
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2423 a         2056 c         1315 g         2804 t
ORIGIN      
        1 accttatctc tggcatcttt tcatccaatg ggtcacatct attgacaacg cagacgcacg
       61 gcttttcgtt tgggagcatc agacatacca ctcacgattt catgtacttt ttcttttgtg
      121 agtgttcctg ctgcgttccg aagttgtttt gcaatcttca tatcaatttt gaaattcttc
      181 gcttgttcaa aaacaatacg ctgttcttct tctgacaaga aggaaagttc tacaccactt
      241 ctaacggcaa gcactttact atcgatccat tcttttagat catcattcaa cttattgatt
      301 ctgattagtt gtacaacgaa tgttctgcaa agtccgtagt cagatgcaat gttctttgcc
      361 gtatcgaccc cttcatttga cttttgacgc aatgcgtcaa aagttgcgac nnnnnnnnnn
      421 actccccatt tagaattttc agctctttaa taatatcatt tcgcttccct tgggaaaaca
      481 tttcatgatg ccgttgtgca attacagcag cttgctcact gactttcaga ttattgaaac
      541 cacgctgtat gatgttgctc tcaatcacat acatctcagc ttcttccgcc gtcagtcctg
      601 tcttcacaat tgccggaact gtagcttttc caatgctttt cgcacaattc caacgattat
      661 gcccgatcaa tatctcatat ttctgtactg atgttgacaa cggctgtacg nnnnnnnnnn
      721 cctccatatc ctcctttcgt tctccggtat aaagttggaa tttatgattg tgatagggaa
      781 tcaagttttc aacgggaatc tgttgaatct tattttcact tccttcttca aaagttccaa
      841 gcatcgtttg caaatcaaac gcagctgtca tcacccacac ctccctctat gcaattccaa
      901 taagaatccg cagaatcaaa tccccaaata gcataatcgc aagtgttgat ctctttccat
      961 aaggattcac ttgtattcgg aaattgctcc atccaatcac aaagcagcca gtctttcaca
     1021 tcaacatagt caaaacgttc taaatcaata ccattaccac ctcgaaaagc cgcatcttgc
     1081 acgttttgca tcacgaacaa catttgctct aatgcctttg catatcgctg ttcttctgat
     1141 acttctggat tctgatgaaa agcagacata caacgaagca tttttttcaa atcttcaaat
     1201 ctcatattct ttctccttgt aatagtttct tttgatgaca tcttgcgttc tgtggctttt
     1261 attccatacc agaaatcata aatctaacgc ttttctcata aatgtcacca agaacaatca
     1321 gatcagaaaa attgaatgga taatctctaa gtgcatcttt atgtttctta tcacttaata
     1381 taaaatcagc cttgcagcat agaaactcat cgggaatttg ctcgccagaa caaattccta
     1441 agttatgtaa aacaattctg ctaagctgca tctgcatatt agcgataatc ggattattat
     1501 ttacattatc aaagatcgca agcagcttat cccaaccacg ttcacttata tttgcagttg
     1561 ttctacatga ttgcctttca acaaactttt tctcatatcc acaaaggaaa agaatgctgc
     1621 aattggcgaa tacggctatc tcattcagat attcatcctt taaggggcta cctacctcta
     1681 aggaaatagt tcttgctgaa gtcaactcat ttccccacaa ctcctgactt accacagcac
     1741 ctacaccatt atcactatgt tcttctccca aaagaaaccc aactgtacac tcaagctttt
     1801 gagaaatttg aatgagcact gcataactag gattcccacc atgtttccag ttggtcacat
     1861 tacttttact caatcccaga tctgcaacta gagaactggg cgaatatcct ttttctttgc
     1921 agcgtgtttt gaacgcatta taatccacaa atctcactcc tcattttctg aaaaccggta
     1981 tctccaaaag tcatacatat ttcttgtaat cttatgaaat cgaatttctt cctgcttttc
     2041 ccaccacaat ttcaataaac tctgtagttc attatcttca aactgtaaaa gaaacttccc
     2101 gtcttccttt ttaatcttta tcccatgaat gtcttccaat gcaaacagca gctgcatggt
     2161 gtcggctata tctgaaacac aaaaagcttg taatgaaacc tgcaaaacac ttgcaagttt
     2221 ctttttcaat tgcatattgg gatttctgga tccctgttca tactgtgcca ctcgcacacc
     2281 ggcagtattt tcaccaaatt caatcgccat acctaattca gcttgtgtca annnnnnnnn
     2341 naatacggtt tccaagtaca ttttttccac tatgcctttg taagtttaat gcagcctttt
     2401 ctacattctc atcttcatga agatagtaat atctcatcca ctgctctatt cccggataca
     2461 ttttttctat gacagtaaaa agaaaacaat ccatttcttc aatgttttga atatcctgat
     2521 atttttttgt tttcccctca ttccattcat gccaaatcaa attcaattca tctgcgagtg
     2581 gaaccagcag atgctgattt actgcatata aaatctcatt gtacatttcg ctataagtca
     2641 taacgggttc tttatcgatc tcccccattt tgctgaataa aggctgctct gcccatttca
     2701 attctcgaat tacatcgatt atgaaaccgc ctattgcaat caactgtttc cattcaggaa
     2761 gatctccatc caaatacaac tgaatcgact tcaattttct gactagattc tcattttgtc
     2821 cattcttgtt gatgtattcc ttcgtaaatt ccatgttttc tcctacctat aaatcaaatc
     2881 tctgatggtg ctaccctgtg cagcaatttc attctctcct ttttgatttc actgattgtg
     2941 agatctgctt ttatcagtaa atccatacaa gcccacaaca gccgtttggt atcaaaatct
     3001 tgttcgcatt caaaagtata cgcactgatc atgccgtctt tatccaattc catatgaatg
     3061 ccctctacaa gctgctggac aaaaatctgt atctcatttt catagctatt tcgcaaaaag
     3121 catctctgaa aacaatcttc gtctgtttct gaaaaggcag atgtccgaag aaatccaaat
     3181 gccaaatcat tgttgctatc ttttagatac catttctcgc cattcttctt ttcgtcataa
     3241 gtcttaattc tgatttgttt catcatctgc cccccctaat tccctgtaag tcatcatact
     3301 gtatgatctc ctctgcaaga gcagcatatt ctgctcccaa ctttgtacgt acagcaatca
     3361 tgtttccagc aactgccgct tttgcagctt ccacactgtt gtgaataaca gtatgaaaca
     3421 atacatcctc gtattgctgt cccatttgcc acataacttc tcgactgatc gttgtccggt
     3481 catttaccat cgtcggcaaa atccctctca tacgcagcgt aggatttacc atagagcgaa
     3541 tttgctgata gaggttcata agagaacgca atccgtccat tgcaaatttt tgtgactgca
     3601 caggaacaat catttcatca gctgcaacca atgcattgat aagaagaatc cccaaagagg
     3661 gaagacaatc caccaacaca taatcgtact tctctaccac ctccggcaaa agaatacgct
     3721 ttaagacgct ttctcgtgca aaagccggta tcattctcat ttcagcactt gcaagattta
     3781 tatcagaagg aatatagtcg atgtgatatt tcacattgca atagatgcct tgctgcacct
     3841 gtagaggcgt caccatactt tgttcgatta cttcaagaat caaatctgta atcatcacat
     3901 cgccaatttc aaatccaaga taagctgtca aatttgcctg tggatctaag tctaccaaca
     3961 gtacctcttt ctttttggct gccaatgctg ccgcaaggtt aaacgtcgta gttgtttttc
     4021 ctacaccgcc cttctgatta caaatcgcaa ttatctttcc cataaataca cactcctttt
     4081 agttcaccat gttccgaagc atttgcatct gcattcgcag ctctgttact tcatcctgca
     4141 agttttcagc tgtcttcttg gcttgctcca actcacgccg ttttttgtca cgataccttt
     4201 tcgaacgaat ccgactattt tgacgatcag tcagtttttt acactctgaa cagtactgct
     4261 tcgaaatcaa cgcaaaataa tctgtatcat agacatttcc gatctctttg ccgcaaattt
     4321 tacagcgttt taattctaac attgcaaact cctttctgtt tccgttgcat cacgcaaaca
     4381 ttctaaaaac gattgcgttc gtcacggata catctgtttc agttcgtcac gaaatcattc
     4441 caaaagcctt ttcattcgtc acgcaaacgt ttttttctcc ccgtttgtgt gacgaacagt
     4501 ccgatttagc cgcttttcaa aagtctccga aggagagttc tttgacgagt tcgctcatgc
     4561 attaaaaatt gaatcaattc gccgcaagta gtgttctcgg aaaaatctcc atctaaccat
     4621 aattccaact ctttttcttc accattacaa tcaaaccatt gcaatgtcag cacaccctca
     4681 cgatttcctc gacgatacat ctgcatatcc gtaatcatat cttccaaact ttgaatatcc
     4741 tcattaacca tagacatctg ttctactctt ttttgtgctg caacataatc aaagtatccg
     4801 aattccgaat cacgctcttc cgataaaagt cgatgtgcca attggcgtaa caatcccatt
     4861 ttctcacctc atgtaaaaat cagtttctac taaaacggaa cttcttcgtc tgaaagaatt
     4921 tcttcaaact cggataaatc aagatctgaa ctccaaccat tttcctctgg gtgttctgcc
     4981 gtatcctgta ttgcagaatg ctccgtttct ttcatgtact gctttgattt ttctcctgta
     5041 aaatagatat tttccgccag cacttccatc gtaaaacatc gctttccgtc tttttcatat
     5101 gatcttgtcc gaagcattcc ttccagtgca atcatactgc cctttctgaa ccactcccac
     5161 aaaaacttcg ccctattgtt ccatgccaca cagcggataa aatcaacctg ttctttcttt
     5221 tttcgctcac aggcgattac tacattacaa acacatttct gcgtttccgc atcgatcata
     5281 tgcagttctg gatcagcggt caaacgtccc attaacacaa catgatttag catttactat
     5341 catacctttc tactcatatc tactcataaa tttcaacttc agttacccaa ctcaacaact
     5401 ggatcccatc ctctgttaat gaatactgtt gggtgtcgtt tgctgtcatc aatcccagat
     5461 acaaaagttc ctgtaaagca actacattac tttcatctct acagatgcaa ttttgatgaa
     5521 tctgaaaaga cttgtacttc cacccacgca aacgggttcc attaaaagaa attgtacgtt
     5581 tcatcaactc gatctgttcg aatgtgatat tgcaataatc ccgataccgc ataaactttc
     5641 tccaacccct ctttaataca attcatcatc tttcttctgc tgattctcct gcacttgctg
     5701 ctgactgtga agatattcct caatagtagt acgtgcataa tccaattctt ttaactcggc
     5761 tactacttga cgaaatactc cattttgaac atttctctgt tctatcagct tatctcgctc
     5821 ctgtgaaagc cgttccaaac ttggaatctt ccggtctggg aatcgttcca aaagagccga
     5881 tttagcatct gaaaactgtt gcagctcttg cttgtgctcc ttttcatact tttttcggaa
     5941 tgtagcactc tgatacgcat cataaaacgg cttgtacttt ttgtatttct tcacaagctt
     6001 tattgtatct gtcacagttt caatctgacg ttgcagcttg ttcagatctc ctactaactg
     6061 cacacgctgc ccatatgtca aaatagaccg ttgttctaat tcttcttgag ttgcaattcc
     6121 tttttctgtg aggtaattca gaatcttaga agcctctttc atattctgaa tttcagccca
     6181 tcttgacaat ccctttgcct gttgaaactt ctccgcagaa gtatcataca aacgagtctt
     6241 ctgcgtggac gttgttcgtc cagttcgcag taactgataa ttgtcaattc gctttcgaat
     6301 ttgtttttcc tcataatacc agcccagtgt tcgagaacga caaaatcgct tcataccctc
     6361 taagcaatat ttcaaagaaa tcacattctc cggacgataa actacattca catcttgttg
     6421 ctttagctga taaaggaaat catcgaaacc agtgcagttc atgattgcag catcaatctt
     6481 atcttttagt tttgctttcc atgaacggtt ctgtcgagcc atgtcccatt cataatgact
     6541 cttacctttt ccttgtttcg gattttcaat tacattcaaa ccatattctc tgcacaaagc
     6601 atcactgatt tcctgcaatc tttccttttc gtgcccacct cgattctctt gatattcgaa
     6661 gcttcgaaag gtttccatat tggtattgca aaagataata tgattatgaa tgtgatcctt
     6721 atcaatgtgc gttgcaagca catattgata atcatcatgc aaaaagcttt ttacaagttt
     6781 tctgccaatt tccattgctt catcaggtgt gatttcatcg ggcaaaaaag attgtatcat
     6841 atgcttggca agcacatctc ctcgaccagt tcccatgctt ctggtttcca taaaatcttc
     6901 tgcaattaaa tttgcctgaa atggtgcaca gcaaaatgtt tcaatcaatc tgccttgttc
     6961 agttttttca ggtttcatga tgtaggcaat tgcttttgac agcgtagaac ggattgaatg
     7021 gattttagtg accgccatat ttcctcatac ctttcttgca attccacaaa atcctccttg
     7081 tacagaattc cactttgatt cactcgaatt gcaatttggt ttacattgtt cccgatacgc
     7141 tgtaaacttt gctgcaagtc tttcagcaaa gccgaatcat aacgaatgat cattcccgta
     7201 attgccattt tgcgaagata atcagacatc gtagagcaac cagaacattg caatttctgc
     7261 atgatgtatg catattcttc ttgcgtaaca cggcacttaa tgcaaactgt tcgatttcca
     7321 gaaaaatccg acatgatata tcacctcctt tctttcaaac aaggtgtttt tggttttggg
     7381 cttttggaat aaaagctgtg atattctatt tttcacgaag tgcgaaaaat gcaaaaatgg
     7441 ggttttaggg gtatccctta acaagcatga ttttcgaaga aaatcgctat ttgtggcaca
     7501 aatagcttgc ttgctactct ggggaacgcc ctacgggtgt tccagccacc cacgggctct
     7561 gcccgttaag attagggaac caatccgcta aaaaggatac tctctgccca gcaagcaaat
     7621 tgttgcgtga caacaaaaat gcagatagag cacacaacaa tatgagctgt ttcagggaaa
     7681 agaagatttc ctgataaaaa ggctgtttgt caaaaatgca ccctcactat atataccgaa
     7741 tccaacaagc aagttatcgg tacttttttc agaaaatctc aaaaattgta gaaatacaca
     7801 aaaagaattt tttaggattg tagatatagc acaaaacaaa aaagagccat tctcaaataa
     7861 gaatgactct ttcaaaaaga tatagaaaaa aagaaaatgg actccagaaa aaatccagag
     7921 tccataatct gtgcggcagc ggcatcttca ttgcatcaga tacctgtcaa tcattttgca
     7981 gaaaaaagca aaagaacgga tagaatccga ggaaattgct gattctccgt cattttggga
     8041 cagaaaggga gcaatatcaa cgctttttct aaaaaatgtg cctaatattc tcaaatatca
     8101 caaagttttg cttgacatca taactgaaat gtgatacaat gataaacaaa agagcacccc
     8161 cacagggtat agttgcgttg acgcacaact atacccttac gccagacaga agacctaacc
     8221 cgtattctgt actgacttgt gagaatgctg ttcatgtatc cgttttgtaa gtggctgtcc
     8281 ggtcactcaa aatgcgatgc tttgaataga tttttgcgag cctgtccaga gtaccattct
     8341 ggcaggcttg ctcttttttt aaacaatccc aaacaatcaa acatcttata tcagcatttt
     8401 ttgctgacgg tactttctgt gcattcgttc aaaccgttat catcaaacga attggtttgg
     8461 actcagccgc tgcaaagtag tgaggatgtt gccgttcggt ggtatgtgaa gcgttctatt
     8521 cagacgcttt acaccatttt ttatgataca cagtctttcg actgattgat ttcaaaaaga
     8581 gcattcaggc aaatcaacag ctaaacttgc acctccagct gtttcact
//
LOCUS       DAWBGR010000103         8520 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2255_length_8520_cov_4.348021, whole genome shotgun sequence.
ACCESSION   DAWBGR010000103 DAWBGR010000000
VERSION     DAWBGR010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2255_length_8520_cov_4.348021"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6045..6054
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2407 a         1842 c         1898 g         2363 t
ORIGIN      
        1 tcgctttgca tagattttct tttgaatctt tgtctaactt tttggggtca gttcacaaac
       61 cctgctccgt tttcttgaat gaacgggttg tgcggtgtcc gcgaaattac aatttgcata
      121 ttctcacttc acaaagaaag gagcatctct gtatgcgtta tttttacaac agctgggatt
      181 cggcgtataa gtcgcacttc ggtgccattg ccagaaacca gagctgtcag ttcaccattc
      241 gcctgccgga agaaacgccg ctggactttt ctccggtgct ggtggttttc cgcaccggct
      301 tcaaggaacg ttttctgccg atgaacgtga aagaacacga aaacggctat gtcacataca
      361 ccacagatct cattcccaag tactccggcg tacactacta ctatttctcc tacacctcca
      421 acggtgtgcg gcactatatc aagcggggcg atgcctgcca tggcacactg gatcagggag
      481 aaatgttcca gctgacagtc tacagcgagg actatcagac accccagttc ctcaagggcg
      541 gcgtgatgta tcagatcttc cccgaccgct tttgcagcag cggcaagatg catgacaatg
      601 tgccctctga ccgcgtgatg cgggaatggg gagaactccc ctactacaag cccgaccaga
      661 acggacacgt ctggaacaac gactacttcg gcggcgatct ggacggtatc cgcagcaagc
      721 tggactatct gaaagatctg ggcgtgacct gcatctatct gaacccgatc ttcgaatccc
      781 acgagaacca ccgctacaac accgccaatt accggaaggt agacccgctg ctgggcacca
      841 acgaggactt tcgggaactg tgtaaggctg ccaaaaccta cggcatttcg gtaattctgg
      901 acggcgtatt ctcccacacc ggtgcagaca gcatctattt caacaagttc ggccgctatc
      961 ccacccccgg tgcgtatcag tccgaaaaga gcccctatta ctcctggtat cacttctggg
     1021 gcaacaagga gtatgagtct tggtggggca ttgacaccct ccccaacgtg gacgagaaca
     1081 accccagcta taccaagtac atctgcggcg atgacggcgt actggactac tggctcagtc
     1141 tgggggctgc cggataccgt ctggacgttg ccgacgaact gccggacgaa tttctggaca
     1201 acctgcgtgc ctgcgtcaag aagtttgacg aggaaaagat cgtcatcggc gaggtctggg
     1261 aagacgcttc caacaaggag gcatatggtg tcaagcggcg gtatctgctg ggcgaccagc
     1321 tggacagcgt gatgaactac ccgttccgcg aggcaattat tgcttacatg aagggcgaag
     1381 ctgccaccac attccgcgac cgcatcatgg gcattctgga gaactatccc aagtgcactg
     1441 tcgatgtgct gatgaacttc gtttccaccc acgacatcga acgggcgatc aaccggttcg
     1501 gcgggcagcc ctgtgacgac aagagcaagg actggatggc atcgaatgaa ctgtcgccgg
     1561 aggaatacga acgcggcaag acactcctga aaggggctat ggtattgcag ttcttcctcc
     1621 ccggtgtacc cagcatctac tacggcgacg aagccggctt gcagggctgg aaggatccgt
     1681 tcaaccgccg ctgctatccg tggggacagg aggacaccga gctcatcgac tacacccgcc
     1741 agctggcaaa gatccggcgg gaacaccctg catttgcaga gggtgctatg gaatttctgg
     1801 tgctggagga caatgttgtg ggatttgccc gctatattcc ggaaaatggt tccagtgctg
     1861 tgatcctgct gaaccgcggc ggggatacca caatggtttc gctccgcgac aaggcattca
     1921 gccgctatgc attcagcacg gtcatcagcg gcatctgcga cggtgatgcc gtgatcctgc
     1981 cgaagaacag ctatgccgtg atcagtgccg tccgcaccaa tgtgccgaat gcacgataac
     2041 aaacacagtg ttcccctaga caatcaccgc tagacaatca ccgcgagggc agccgaaagg
     2101 ctgccttttg cttttatctt ttcacacgct ctgattcgac aaattttcta tagtttgatt
     2161 tgaaaattga ttgttttgca atgcgaaact tttctttttt cgaaagtagt gaaaaaggct
     2221 ttacaagtgc ccgaaaatgt gatatactaa tgaagtcgcc aatcgaggtg tggctcagtt
     2281 tggtagagca ctacgttcgg gacgtagggg ccgcaagttc gaatcttgtc acctcgacca
     2341 attgcagaag gctgaattac gaaaaagctc gtaattcagc ctttttttgt tacctggggt
     2401 gttttgcgaa aaacaggttt tttcccaaaa tggcaagtaa atacgggcaa atggcaagta
     2461 aaatggcaag tgggaaacgg gcagcttgtg ccagttgttg gaatttttag agctgacagc
     2521 gttgtccaat caagaaagaa ggcgaatgga tttgaacaaa aatatgaaaa gcatacagcc
     2581 tatagacccc gtcattacac ttccagccta tccgaatcaa aggcaatacc gtgcaaagtc
     2641 accagacgct ctttgtgcgg tattgcctta aaataaaata ggcaaaggat ttgtcactgc
     2701 caatcgcagc aagtcctttg ctttttatta tttccgttcc gaccgcctgt gatttctgta
     2761 ctccttaggc gtcattccat agagtgcctt aaatttgcgg caaaaatatc ccacattttc
     2821 aaagcctact gtctgagcta taacgaaaac gctgttttca gtttccgtaa gaaacttggc
     2881 agcacgcttc aaacgatatt ccagagcata attaatgggc gatgtatgca gatagctttt
     2941 aaaaatattc agcgcactgc ttttgcttat agaaacagat cttgcaatgt cttccaaggt
     3001 tatccgacga taatagtttt tgtggatatg ctgcatcatt atctgcagct gtgcttgtgc
     3061 ctgtgcggag gttttgttta tgttttgagt gtcgttccaa cttatgcttt cgtaaatctg
     3121 cccccatagc ttcataataa gttcggttgt tttcagttcg gatatgcttt tcgattcctg
     3181 tatggcaaac acatttcgta ttgtatcaag catctcacat tgtttttcat ctgtcggcga
     3241 tattatcagg cattcaaccg aggacataag cacaggcttt atatatttgc ggtatatcaa
     3301 actgttttct gcggccatca gaagcggaga aaagaccata ttgggaataa ttgtactttc
     3361 gctactctca aaccgatgta tcacctgtgt gttgatgaaa attgcttccc ctgtgttaag
     3421 cacatatctg ctgctgccca caaaaaaatc tgccgtccca ttttccacaa gcacaaactc
     3481 aacctcgggg tgccagtgcc agtcaataca gtgcaggtca aagagccata tatcctcata
     3541 ataatatcga aagggatatt cctcactgcc gtgacttatg gtttcacgaa gagtttcgtc
     3601 agtgatgatc tttgcgtagc cgtcgtttag gtttttcaaa tttatcctcc atttttgcga
     3661 tatagtgtaa taaaaatgcg atcttgtaca ttgaaatatg ccgtttgtta tgatatgata
     3721 caattatatc aaaaagcgaa aggaacgtca agatgaatta cagaaaaact cttattgcct
     3781 gttatcttgg atttattaca caagctatta cagcaaattt tgcaccgctg ctatttctca
     3841 cattccacaa tgtttacaat atccctctcg gaaaaattgc gctcatatca tccgtattct
     3901 ttatcactca gcttattgtg gatattctct gtgcaaagtt tgcggacaga ataggctacc
     3961 gcagatgcgt tgtgggttct cagctgtttt cggcagcagg acttctggga cttgcgtttc
     4021 ttcctcagat aatgcccgac ccgtttatgg gaataatcat cagcacaatt gtatatgcgg
     4081 tgggcagcgg tcttacggag gtgcttgtca gccctatcgt tgaatcctgt ccctttgagc
     4141 ataaagaggc ggcaatgagt ttgctccatt ccttttactg ttggggctcg gtaggagtta
     4201 tcctgctttc cacagtattt ttctcggtgt tcggtatagg caattggaaa tggcttgctt
     4261 gtatctgggc aataattccg ctgtataata tgtttaattt catcacctgt cccattgaac
     4321 accctgccga ggacgggcaa ggtatgagta tcggaaatct tttaaaaacc cccgtgttca
     4381 ttatttccat actgctgatg atatgtgcgg gagcgtcgga gctttcaatg gcacagtggg
     4441 catctgcatt tgcggaatct gctttggatc tttcaaaggc tatgggagat attgccgggc
     4501 cttgcctgtt tgcggtgact atgggaataa gtcgctcact gtatggaaaa tacggcgaca
     4561 agcttgacct aataaaattt atgataggct caggcatgct ttgcctgata tgctatcttg
     4621 ttgcttccct gtcggatatt cccatgcttg ggcttgcagg ctgtataata tgcggctttt
     4681 ccgttggaat tatgtggcct ggaaccataa gcatctgttc ggggaaaatg ccctcaggcg
     4741 gcacagcaat gtttgccctt cttgccatgg caggcgacct tggcggtgca ataggtccgg
     4801 gtatcgtagg aaacatttca caaagtgcag gagatgatat tcgcaagggc gtgcttgcag
     4861 gctgtatttt tccacttgtg cttgttattc tgacttttgt gatttccaaa aatgtcaata
     4921 aaaacaggca ggaatgaaag agtatgacta taaggcaata ccacacaaag agcgtctgac
     4981 gactttgtgc ggtattgccc tgaatcagta caatgtgatt catatcgata tccagcagtt
     5041 tgtcagcaaa tatcctgtga taaaagacat ggtggcacgc ttaacaaagg aattgttgtt
     5101 tgatgtgaaa atggaaatgc cggatgttac attgtttgat gaaacagact tggtaaggac
     5161 attaaaagat attttttcgc agaaacggga aaagtttatc tttatcattg acgaatggga
     5221 ttccattttc cgggtacatc gggataatgc aactgctcca aaggaatatc tggattttct
     5281 gcgggatctt ctgaaagggc agccctatgt ggcacttgcc tatatgacag gcattctccc
     5341 aatcaagaaa tacggtcaac attctgcact gaatatgttc aaggaataag ctatgaccaa
     5401 tcaatatgca ctcgcaaaat tcactggttt tacagagagg gaagttcgtc agctctgtga
     5461 acgttatcat atgtcttttg aacaaacaaa agattggtac gatggctata ctgtcaatgg
     5521 tatatccatt tataatccaa gatctgtaac atcagcgatg atgaacggca tctttgacag
     5581 ttactggaca cagacagaaa cctatgaggc tttgaaaatg tatatcgttc gcaatgagaa
     5641 cggtttgcgg gataaaatcg ttcggatgat tgccggagaa catatttcca tcaatacgaa
     5701 aacatttcag aacgatatgt gtacttttga aaccgcagat gatattttga ccttactggt
     5761 gcatctgggc tatctgacat acgacttcga tacaaaaacc gcctggattc ccaacaagga
     5821 agtgcggcaa gcaacttttc catcggaaaa aggtcttgac aaggtactgc aattgtgcta
     5881 aaatgagaac ggaaagaaat aatgtagtgg caatacagtc acgcaataag aaagccccca
     5941 gagttgcgac ctctggggct ttctttaggc tagttgtcac tgtcgtctcc gtctaaccat
     6001 ttgcagatat aataggtgac tatacctgcc acgacagaaa caaannnnnn nnnnagttgc
     6061 gacctctggg ggctttcgcc ttatcccata caagaatcag gcaaaatgtt gtgctgttgt
     6121 cgggctactt cctgctcagc cacttgtaga agagatataa aatctcatct gctacaacag
     6181 aaattaagag agatattatg taatatagca ttaagcatac cacctcttta tttacagtca
     6241 aaagttttta tagtgcactt aaaaaactct tgattttttc tatcatttta tggtatacta
     6301 taaaaagagg tgattgtatg gtagaacgta agatctatat ggataaaatc aaaaaactgc
     6361 aggaccaaaa aaacttttaa gcacttcgaa ggaagggcat gaaaacgttc ccttgttgtc
     6421 atatttggat ttcagttgat tttctctttg cttcatgata taataaaaac agatagcgga
     6481 ttatcttgaa gaatatcacc ttgagaaagg ttatctgctg agctttcatt tcaacaaaaa
     6541 caaaaaaacc ggactgaaaa ccgcagtcat aaacggcaag actgtgattg aagccgtcat
     6601 ataaaacaac atcataaaca tatcaatcga gagtccgaaa agcatgacgc ttttggactc
     6661 ttttgtttta ttcttccaat gtcatggttg ctttttacca gtattgtgct atagtagtta
     6721 cggcaatgaa attttacaac aaattatgta ctaaggaaaa agaggggtga ccgatcatgc
     6781 acggaatatt tctgggcgga acgtacttgg aaaaacatct ggagagcaca tagagacata
     6841 gttctagaca agcaggaact atgggtgctt aacacttgtc tgtttctcat tctcgattac
     6901 ttcggcaata gaaacggctt ctatgacagc attatttcat gataataacc ttactgtttc
     6961 aagtgaaaaa tgatattgca gagtcaaaaa ataagcttta tcaaaacatt tatagagaaa
     7021 gtctgctcag agcaggcttt tttcttactg tgcctgcatt caagttgatt ttcacgctgt
     7081 ttcatgatat aataaatgca agtgagaaat cgttataaga ctatgaaagg agttacacca
     7141 tgcgttccga aagaaaaatg ctccccatcg gcgttgagaa ctttgaaaag cttcgcagaa
     7201 acgatatgta ctatgtcgat aagacagaga tcattgcaga cctgcttcgg aatcatgggg
     7261 atgcaagtct gattacccgt ccccggcgat tcggcaaaac actgaccatc ttaattttta
     7321 tatttgatct gtattgtata tttcatcaca cgaaaattga acggaacagg agttcgataa
     7381 tatgccaaaa tcagaaaatc aaaaacaaaa gcttctgtat ctgctaaaat tcttctatga
     7441 ggaaactgac gaggatcacc ctcttacggt taacgaaatc attgcaaaac tgaaccagaa
     7501 cggaatttct gctgcgaggc gttctattta tgacgatatc cgtacgctgc aagatttcgg
     7561 aatagatatt gttatacgaa aaagtaagac ttgtgcgtat tttttaggtt ccagactttt
     7621 tgaaacatct gaactgaaaa ttctcattga cagcatacag tcctccagat ttattaccaa
     7681 aaagaaaagt gaatctatca taaaaaggct gaaacagctc gcaagcaaac cacaagcacg
     7741 gagtttttca ggacaaatat atatttacga tcgtatcaaa agcatgaacg aaagcatttt
     7801 atacaacgta gatacgctgc acaacgccat tgcagcaaac cgcaggatca cgtttcgata
     7861 ctttgattat aatataaaaa gagaaaaagt ttttcgtaag aacggcgaat tgtattctgc
     7921 tgatcctctc gcactgacct ttgacaatga aaattattat ctgatatctt atcatcacaa
     7981 gtatgagggc tatgttcatt accgtgtgga taagatgact gatattcata tcagccctga
     8041 aaaaagaagc attcccaacg aacgattcaa tgccgcagca tatgtaaagc cgatgttttt
     8101 tatgtttgat ggtgaaatcg agcaaatcac agctttattt catatatcct atctgaatat
     8161 tatactggat cgatttggtg acaatgtgat tttacatgag acggaagatg cagaatgctt
     8221 tcaggcaact tttaaggcgg cggtaagtcc gacctttctt tcatggatta tgggattcgg
     8281 ttcaggtgct ctgatccttt ctccacagtg ggttgccgat gaattaaaac ggctttcttt
     8341 ggaaacagca gaaatgtata cggacatgaa agaatcatag tactgtttat agtacacctg
     8401 tcatgatata atgatagcaa cacctgagga aatgatagta ctaaagtgat ctactaagga
     8461 aacattgcat aaagcccgcc tgacggcttt gcacgcaatc tgcttgcaga ttttccgtca
//
LOCUS       DAWBGR010000104         8508 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2261_length_8508_cov_4.340826, whole genome shotgun sequence.
ACCESSION   DAWBGR010000104 DAWBGR010000000
VERSION     DAWBGR010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8508
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2261_length_8508_cov_4.340826"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6461..6470
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1879 a         2215 c         2223 g         2181 t
ORIGIN      
        1 gacgaaaaat ttgacgacgc atctgtcgca caatctttgt gcggtattgc ctaaacaaca
       61 aaaatccgcc tgaagaaaac acttcaggcg gattttttta tatagggcaa tgcttcggtc
      121 agtttttcag aacgtcgctc catgttgcct tgattccggc tccatgcatt gcgatgtaca
      181 gcagcaaata gtagagatcc tgcgaatcca gtttattgtc attgttgatg tccatcatca
      241 ggatcagcca gcggttgacg gtcggatcct cgtggaaaac cacgtccttt tcgccctcag
      301 ctgttctcgc aatatacagc agcagttcga aaacgtcctg cgagtccacc tcaccgtcca
      361 tgttggcatc accacgggta cgcggcaaaa taattggctt ggtcgtcgtc gcagtggcag
      421 ttgtcgtggt ggctgcggtg gtgttggtgg tagttccgga actggtggca gtactggttt
      481 ccgacgtggt tttttccgca gtcgtcgttg tttccgtcgt cagaatggga atcgtagacg
      541 ttgtggtgct ttccgtggtt tccgtggtac tgctctccgt tgtagtcgtc gtggttgttt
      601 ctgtcgtagt agttgttgtg gtggtcgtgg tggtcgtttc ttccgtagtt gtaaaagtcg
      661 tagttgtggt ggtcgtcgta gtcgtggttt ctgtcgtggt tgtggtagta gtcgtcgttg
      721 tcgtggtagt cgttgtcgtg gtagtagtag ttgttgttgt cgtggttgtc gttgtctttt
      781 tggtgaatgc ttcctctgtg gtagttgtcg cagtcgtggt agtcgtgacc ggcttcttgg
      841 tgaacgttcc ttcggtagta gtcgtcatag tcgttgtcgt ggtgatcggc tttttcgtaa
      901 acgtttcatc ggtggttgtc gttgccgtag tcgactccgt ggttttcgca gtggtgcttt
      961 ccgtagtcgt cagcgaggta gtcgtttctg tggtagtagt ggtagtagtt acagtcgttt
     1021 ctgcggtggt ggttgtcatt tccgtcgtag tttcggtggt tgtttctgtg gtagtcgcag
     1081 tggtttctgt ggtggtcgtt tcggtggtag tcgtttcggt ggtggtttcc gttgtagtgg
     1141 tggttgttgt tgtcgtagtc gtcgtcgttg tggtttccgt tgttgtgacc ggcttttcct
     1201 tggcagaaac gacaatggtg ccgatcgcgg tttcatagtt ggaactgtcc gcgggaacga
     1261 acttccattg cagctggttt ctgccggctt tgaaagtgct cggtgtgtct gccgtccaca
     1321 tcagaatacc ctcggtattg gcagtgtcca gccgtatcgg cgacacgtca tcgccttcgg
     1381 tatattccgt ggaatcgtcc catatcacag tcaccttggg caccgctttt tcaatgacaa
     1441 attccagcga tacgctgccc gaccagagag agccctctgc cccgctgacc agaatcgttc
     1501 ctctgccggc gttgagattg ttctcatagg aaagcgtgta atccgtgccc tgtttcagct
     1561 cgttgcctcc tgcaactacg atcacgtccg gtgtcagcgg ttcgccggtg tagaaaatgt
     1621 tcttctcatt ttccagcgtc acatcgacga tgttcatggg aatgtcttgc agctgcacag
     1681 tgaaagatgc agtcacattg ggatttcctg tgtgataagt ataagtgata ttttgcagcg
     1741 gatctgttat ataaaccttt gtgccgctgc attcggcatt gttccacgtc tgtacaaggt
     1801 ctaaccggaa cccgctccac tgcgtcggtt ccagatacag atcttttctg ttgcaggacg
     1861 gctttttcag aaccacacaa ttttgcagat cgcagacagt catcggtgcc tgctccgaca
     1921 ggttcagtgc cagcagcgga ttgctgccgg cgtaaagcat atccagctga tgcagattgg
     1981 tcagcatcag ccagcccagc tggttgccgc tgcaatccag ttcttccaga gcctgctgat
     2041 tgttgatgtt cagagaattc agctgatttt ccgcacagtt cagcacccgc agtgaaaccg
     2101 ttccccacgt gaagcctgtc agctgattct tgcggcagtc cacttccttc agtgccgtac
     2161 agcgggacag atccaaggtt gtcagctggt tattgtcaca aatcagcttt tccagctgta
     2221 ggttggacag atacagagaa gtcagctcgt tctctccgca gtccagcaat tgcagttccg
     2281 gcagaacatt gacgttcagg ctttgcagat gcaagccgcg gcagtgcagc tctgtcagcc
     2341 gatagaagtc cgtcaggttc agttcccgca gtgcggtatt gctacagtcc agcacctcca
     2401 atttctcgga aattccggac agatcggaga tcgaattccc tgcacagttc agctgcacca
     2461 gattcgggaa gtaagaaatc cccgtcaggt cggcaatgcc gcagttcgaa atgtccagct
     2521 gtgttaccgc ttcctgctcg gaaatttgca gcatgccgtc cccgtttttg tccagagatt
     2581 gcagcacata ggaacgaagc acatcgtccg gaaaatgctc tgtatcaata gcaacggcat
     2641 cttcttccat gactgtggga atccatgtga acgataccgt ccgactggca tcaccggcat
     2701 cgatctggta cgcatagcgt atctcctgcg aacggtcgtt cactgtcagc acattctccg
     2761 tacaggtggc attctgccac atatccggct gcacctttga cggctggaaa ccgtctgcaa
     2821 gggcagacaa atctacctgt cccatggcgt taaatggtat gcttcgggta ttgtcgatca
     2881 cttccacctg ctgtaatttc gacaagccgg tcacgtccag tgcgatcaga tgattctcca
     2941 tgcaccgcag tgtttccagc tgtttctgtg ccgaaacgtc cagtgtggtc agctggttgc
     3001 cggagcagtc cagcgtttgc agctgtgtca gtgtgcccag ttccagcaca gtcagctgat
     3061 tgccctcgca cgccagtttc gtcagttccg tcaatttgga gagttccaac gccgtcagct
     3121 ggttcgatgc acagctgagt tccttcagca atgtattggc agagcaatcc agcgtggtca
     3181 actggttatt gttgcagttc agcttttcca gctgccccag cgtccgcagg ggaattgtcg
     3241 tcagcttatt attggagcag tccagcgttt gcagtgctgt aaagtattcc acgccggtca
     3301 ggtcggcgat ctcgttgatc gaaacgtcca gttctgtgac ttgtttcagt tccgaccggg
     3361 aaagcagtcc gtcactgtcg gtgtcatacc ggacaacgat acggcggaaa ttttcgtccg
     3421 gaaaatactc tgcatcaatg ggcacacccg tttcctctgc tgcctgcggc accagtgtaa
     3481 atgtgattac actgtccgga tcatctgcct gcacctgata ctgatatgac acactccagc
     3541 tggggtctgt gacatagata ccgcctgcac ctgcctcacc attggtaatg ctgccctgta
     3601 ccacgcggga cggctgaaag ttctccgcca gatctgcaaa gcggatccgg ttcaggctgt
     3661 ccattgcaac agtacgtttg ttgcgtgccg cactcagctt ttgcagtgtc acgccatcag
     3721 ctgcttgcag tgcagtcagc tgattgttga tgcacaccag cgattccaga ttcggcagcg
     3781 actcggtttt cagcacggtc agctggttgt tggaacaatt cagtgtagcc agttccggac
     3841 actgttccac cgccagtgcc ggaatctggt tgctggcaca gttcagggat ctcagcacgg
     3901 aaagagctga taagtctaaa gcagtcagct tattcatgga acaattcagc gtggtcaggt
     3961 gggacagtcc gcccagttcc agcgaagtca gctgattcag tgagcagttc aatgtcgtca
     4021 gttccggcga attcgtcaga tccagcgttt tcaggcggca ctgggcacag tagagcactt
     4081 gcagatgaga aagagaagca acagagagtt cctgcaatgt gctgttgctg gagcagcgga
     4141 gttcctccag aaggctgttc tccggcagta ccagcgagga gagattatta ttggaacagt
     4201 tcagcgtttt cagttgagaa agcgttgtca gatccagctt ccgcagattg ttagcggagc
     4261 agtccagcgt ttccagccgg acaaaatact ggattccggt cagatcccag atagacttcc
     4321 cgccgatgag catttctgtt acggcaaaca gttccctgcg ggaaaggctg ccgtcgtcat
     4381 tggtgtccag tccctgtaca tagctgcgga atgcactgtc cggaaaatgt actgcatcaa
     4441 caggcagcag cggttcctct ggctgtaagg taaactgcac cgtctgggtt ctggcggcat
     4501 cgaggtaata ggtataggtc accggctggc taaggtctgc caccgtcaga acgccgtcct
     4561 gcaccgtgcc gtttgtccag ttctctgcct gctctgcgga gaagccggac agttgggaca
     4621 agtccagctg tcccagaata tccggcgaga tcgcagtcac gtttccatca gcagacactg
     4681 tgccgtctgc cagtttcgtg ccgtcgattt gcagggcaca gagttggttg tccgcacaga
     4741 acagcttttg cagattgctc tgcctgctca gttccagaga ggtcagctgg ttttgtttgc
     4801 aggagagcac ttccagcgag gattcttccg gcagcagcag ttctttcagc ccgcagccgt
     4861 cacaggacag catcttcaaa gcggaaaatc cggaaacgtc cagtcgttct aaagaaattc
     4921 cgctgcactg caaattctcc agccagcaca gctgttccat tccggtaagg tctgtgatgc
     4981 cgctgtctgc ggaaatcgtc agggtcttgg ctgcctgtag ttccgccagc gagagccagc
     5041 tgtttccgtc agtgtcaaag ctgtcgcgga caaggctccg gaatgctgcg tccggaaaat
     5101 gctcctcgtc cagctgcaaa taacacgctt cgtccggctg caatgtcagg gtgaacgttg
     5161 ccaccggcac tgcctgctgg ctgtccagcc ggtagccata agacacacgt tcggtgtctg
     5221 ccggaatttg cagtacattg gcggcatctt ccttcacaga agcgttctcc cagccggtga
     5281 tgcggtcctg ttccagttcc ggcagattgt tcaggtcata gctgtacgct cccgcccgtt
     5341 ccggctgcgg cacaatggca cgctgattgc cggcagtttc cagtatttcc agcgtcagtc
     5401 cggtggtgtc cagttccacc agctggttct gggaacagtc cagttcgcgg agagcagaac
     5461 actccgacag gaacagggac ggcagggcat tttccgagca gtccagtttc tgaagaaggg
     5521 cattatatac gtccagttct gtcagctggt tgccggagca gtccacttcc tccagcttgg
     5581 cattctggga aaagttcagc gtagtcagct gattattgga acagtttaca gtctgcaaat
     5641 tggaattcgc agaaacgtcc agtgctgcca gctgattatt ggaacagttt acagtctgca
     5701 aattggaatt cgcagaaacg tccagcgtag tcagctgatt gccggagcag tccacagtcc
     5761 gcaaattgga attcgcagaa atatccagtg ctgccagctg attgccggag cagtccagcg
     5821 ttcccaaaag ggcgtaagaa tccagcgaca gttctgtcag agcacagtca ctacagtcca
     5881 acatcaccaa aaaggaaaac gcaccgtttt tctccggcag tttcagtgca gacaaatccg
     5941 gattcgcaga gcaattcagc tgcaacagct gtgcagcacc ggaaatatcc aatgccgaca
     6001 acgtgtttcc ggaacaatcc agctgctgca atttggaaaa gacatcgatc cccgtcagat
     6061 cggcaatgcc ctgctgggat acattcagat caacgacttc gctgcattcc gtcgcggaca
     6121 gcatgttgtc accgtcgctg tccagctgtt cggaaatgta gttccggaac tgctcgtccg
     6181 gaaatcgttc tgccgaaatg gcgatttcct cccccgccgc cgcatctgct gcaaatgccg
     6241 gcagcggcag gctggtcaca gccatcatgc ccgccagtaa aaagctaagg caacggtatc
     6301 gattcgtgtt tgggttctgc acaaacatcg cttcctctct atgcaggcag cctcacgccg
     6361 aacctacctg acagactgac atgacgctgc tttcagagcc tccggaaagc acttccggag
     6421 gcgttttttt atctaaagtc atacattaat attatagcac nnnnnnnnnn agcaagaact
     6481 tgtggaaaaa ttctgtaagg cagccggtgt ttccgtcatt cctcacaaaa acagcgttgt
     6541 caaattctac aaaaaagcta ttgacaaaag ggataaaatc gattataatg taagaaatat
     6601 tgtatgtata ctcatgcact gaaaggggct tattcttatg agatcaaatt ggagaaaaac
     6661 tattgcaggc atcgttgcca ttgcggcact gattccctgc tgtctgggca cgaccgctta
     6721 tgcagcacct cagagtgccg ccgacacggt gatctcggca gtgatgccgg aactggaaga
     6781 accgatgccg tccagtcagg tcgatgcaca gattgcagca gatgtgaaag cagcgatcag
     6841 cggcactgcc ggcggtctgg aaaccattcc cacaccgaat tatgcggaag attccgacgt
     6901 tgtgattccg gacggcaaac cgacacagga cggcaagctg tactacaaga tcgtatcgaa
     6961 aaaggcacag atcaccggct gtgcaccggg caccactgat ctggtgattc cggataccat
     7021 ttatgacgaa gaggacgaag aagaatatcc ggtaaccttt atcgctgcgg ctgcattcta
     7081 cggcaacaag gaattgcaga ccgtcaccat gccggacggt atggtgggca tcggtgcaaa
     7141 cgcatttctg ggctgtacca atctgactag cgtcagcatg ccggacagcg tggcttccat
     7201 taacggtggt gcgttctgct cctgctccaa cctgacagac atcaagctgc cggcaagcct
     7261 gtactttgtc ggtaacgatg cattctccta ctgtgcatct ctggaatcca tcacgatccc
     7321 cggaaatctg acctccatcg gctctaccat gtttgcaaac tgtgcaaagc tgaaaactgt
     7381 cgttctggaa gaaggcgtac agagcatcgg ttccaatgca ttctatggct gttcaagtct
     7441 ggacactgtg cagttccccg aaagctcctg caccagaatc aacgcaaatg cctttactta
     7501 ttccggtctg tcttctatcg aactgccgga cagtgtgact aatgttgcaa cagcagcatt
     7561 cagccactgc cagaatctga aaactgtcaa gctgtcctcc ggcatgactt ccctgcggaa
     7621 ggacaccttt gcatacagcg gtctggaaag cattgaaatt ccggacagca tcaccacgat
     7681 caaatccggc gtattcgctt attgcagcaa cttgaaatcc gtaacactgc cgcagacact
     7741 gaagcaggtg gacgagatcg tattctacag ctgcggttct ctggaaagca ttgtcctgcc
     7801 ggattctctg accaagatca gtgacaacct gttctatcgc tgcaccagcc tgaatgatgt
     7861 caagttcggg gcaaatgttg actcgatcgg tgcttctgca ttctacggct gcaccagtct
     7921 gacagaagtc aatattccgg ataccgtcac caagctggga cagagtgcct ttgcagagtg
     7981 caccaatgtt accaaaatca ctgttccgga ctctgtgacc gatctcggca agtggacatt
     8041 ctacaacaat gccaacctga ccgatgtcac catcggcaat ggcgttacca atctggataa
     8101 ctacctgttc taccgctgca ataaactgga caatgttacc gttccggaaa gcgttaaaaa
     8161 ggtgggacag tacgccttcg gcggctgcac ttctatgagc agcattcagc ttccggattc
     8221 tctggaatcg atcgacaagt gtgcattcgt agactgcgat tctctcacaa atgtcaccat
     8281 tccgaaaaac gtttcctcta tcggcagcaa tgcattcggc tacaaagtgg aaaaggatac
     8341 gcttgtaaag ggcaatgata tgaccattac cggcaatgag agcactgccg caaaggacta
     8401 cgcaaacgca aacgacatca catttgattc gctggatccg atcacgacta caaataccga
     8461 agttccggta gcaacagaca ccactgttcc ggtagcaacg gataccac
//
LOCUS       DAWBGR010000105         8486 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2271_length_8486_cov_5.255248, whole genome shotgun sequence.
ACCESSION   DAWBGR010000105 DAWBGR010000000
VERSION     DAWBGR010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2271_length_8486_cov_5.255248"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1672..1681
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1783 a         2480 c         2022 g         2191 t
ORIGIN      
        1 acgaaaaatt tgacgacgca tctgtcgcac aatctttgtg cggtattgcc tttattctca
       61 gaaaggaagt tttattatgg gtttcttgac caacaaaacc gccattatca ccggtgccgg
      121 cagagctgtg ctcagcgacg gcagatgcgg ttctatcggc tatggcattg ccaccgccta
      181 tgccaaagaa ggtgccaatc tggtcatcac cggacggaac acacagaagc tggacgctgc
      241 caaggaagaa ctggaacgcc tgtacggcat tcgtgttctg acagtgcagg ctgacgtgaa
      301 cgccggtgcg gacaatgccg ccgttgtgga aaatgtggta caacaggcag tggacacctt
      361 cggggacatt catgtgctca tcaacaacgc acaggcatct gcctccggtg tcacgctggc
      421 agaccacacc acagagcagt ttgatctggc ggtctactcc ggcctgtatg ccacattcca
      481 ttacatgaaa gcctgctatc cttacctgaa aaagacacag ggttctgtca tcaacttcgc
      541 ctccggtgcc ggcttgttcg gcaacttcgg acagtgtgcc tacgccgcag ccaaagaggg
      601 cattcgtggt ctgacacgtg ttgccgctac cgaatggggc agagatcaca tcaatgtcaa
      661 catcgtttgc ccgctggcat ggactgcaca gctggaaaac ttcaagaatg cctatccgga
      721 cgcattcgaa aagaatgtca agatgccgcc ggcaggacat ctgggcgatg cagaactgga
      781 gatcggtcgg gtctgcgttc agctggcttc tccggatttc aagttcatga ccggcgaaac
      841 cattacactg gaaggcggtc tgggactgcg tccgtaaagg aaaccgtcac acaacaccat
      901 atccattccg gcaggggcac acgtccctgc ctttattttt tcttgaacac cagccccatg
      961 gtgaacccgt gcgactggct gtcgttatag gtgatgtggg attgctggat tcgctcccgc
     1021 acgtcctgca ggttactcag ccgcttcagg atcttcgccg tgttcctggc atctgccaac
     1081 gccccgtgtt ccgttccctc gaaatagatc tgcatagagc ccagtgcatt ggtcaggttc
     1141 atctggcggg taaatccata cattctctga tgcagtcgct gcaagtccac ccagtgggtg
     1201 tagaacatat cgtgcagcga ctgttccttg agatctgcct cgttcagaat tacgttctga
     1261 tcggaattgc tccatgtata aaaccggctg cgacagtctg aaccgatcca gtctgtaaag
     1321 ttcgccattg cctctgcgta ggacggagcc gttgccaccg tttcctccgt gattccggta
     1381 agcactgtca cccgcggcaa gactctgctg tactgcggct tgacataagt ctgaaactcc
     1441 tccagaaccg cattctgctc gttcagcttc accgcaccga tctccagaat ctcacttctt
     1501 gcaactgcct gcaagtccgg atctcgaatc ggcgtgaact ccagatccag aacgatctta
     1561 tttccctgca tatgcattac ttcccctttt ttcaccaatt ggataatgtc attatagcat
     1621 atcccagaaa agttgacaat ctattttgac ataatttgcg tttattttga annnnnnnnn
     1681 natttcatag tttttgtttg ttgttttatg attttttgta attttactta ccaaaacaaa
     1741 attttagttt tacacttcca tggaaaaatg ctctggaaca aaccggcttt tatgcttgaa
     1801 aaactgccaa tgttttcctt acactaagcc gcactcgtcg taatctttcc acttttcctg
     1861 attgacatgg cggcggtgtg tggagagagc gtagacaatg cctgccacgc ccactgcaat
     1921 ggcaaccagt accatcagta cgccgcaaag ggacgacttt tcttctttcc gtgctttcat
     1981 tctgccgcac tcccttctgt tgcagaagcg gcatcggtct gccccatctc tgccattaac
     2041 cgctgcagtt ctgcatctac gtccatatca tgctgcaatt ctgcaaaggt cttttcgtcc
     2101 tgtgtgctgc tggaaccggc gatctcggca aatgcctctg cctctgcctc ctgccgtgtc
     2161 accttttctt ccatgcggtt gaacttatcc atggcactgg aagaatccag accgcccacc
     2221 gactgtgcca gattcttctg ggtctttgcc atctgagagc gtgcgatcag cattgcctga
     2281 cggctcttgg cttcgtccag cttggacttc agggtttcca cctgttcctg tagctgtgcc
     2341 gtctgggcag cgatctgctc atacatctgc tgatactgct tgacagactc gtctgccttt
     2401 accttattgg aaagtgcctt ctttgccaga tcaacttcgc cggcatttag ggcagccttt
     2461 gctttcattt cccagtctgc ggaagtcttt accgcatcgt catactgccg cttggcaatg
     2521 cgttcgcttg cctttgcctt gcccagtgcg gtggtggcgt tgctgacttc cttttgcaga
     2581 tccagaatga tctgctttac cattttctcc ggatcttctg cacgatcgat caggtcattg
     2641 atgttagact ttacgacatc gccaattctt gaaaaaatac ccatattgtt cctccatgct
     2701 ctttgagctt ccgctcgtta tgatatgata gcaggttttg acagacccgc ctgtcggctt
     2761 tctgtgaggg ctgccattgc cattttgata tttcacgttt cctatattat atcgtacttt
     2821 ttccgtcttg tcaagagaaa tcttacaaat catttacaaa tccggcagtt gttccggcat
     2881 tttacccagt tttttctgaa aactgtcccg atgcttttcg caggtttgcg ggatttctgt
     2941 tgaaaatcct acaaaacgct tacaatgcaa aaaggggtgt gcatttgctg cacacccctg
     3001 tacgacgcac cattttatac cgtgctgccg tcactttttt caggacaata ccgcacaaag
     3061 attgtgcggt attgcctcag gattcggcgg aatcttccgt gctgtccggc tctggctcct
     3121 gctcctgcat ctgctgcaaa atccgtgcaa ccgcgttgcc gttcagtttc gcggaaacct
     3181 gtagctggct catagcggtc ttcatctgcc gtacatcaga agccaccttg cggaactggt
     3241 tcagatcgtc ctgcatgccg gtcagggatt ctgccagctg gtaaaacttg cggaactccc
     3301 gtttcatgtt ttcaatatcc agcaccattc tgccgaggct ctccacctct ttttcctttt
     3361 gccgctccag ttcttttttc tgttctgcca gttcttcctc cagacgctgc acccgaattt
     3421 ccagtgcccg attctggatc tccagcgtcc ggatccacgc ggaataccgg cggttgatct
     3481 ggttctgccg catgccgaac acatggatct gatgcggcca gactttctgc ttctgggttt
     3541 cccattcttc ttcctcgtag gcatcagaag gaagtgcact ataccccagg tcttcctgtc
     3601 ctgcttccga ttccggcgtg agttccgaag cgtctttcgg ctcctcaccc tgtctttgtt
     3661 ccaattgttg tgccatatgc tctgcctccc cttgggaatc gtttcggaga aacacaaaat
     3721 aaagccgtca ggcgagcttt attccgcatt ccctcaaata tgctgtttcc agtattccag
     3781 tgtttccgca atggtctgtt ccagcgggat ctccggctgc cagccggtgt ctgcctgtag
     3841 cttggaaata tcggcacgga tcaccggcac ttccaccgga cgcagcttgg cagggtctac
     3901 ctccacggtg atctccttgc cgctgtgtgc aatgatgcga tccagaatct cctgaatgac
     3961 aatggctctg ccgctgccca cgttatacac ctcgccggac ttgccgtgct cgatcagcag
     4021 gctgtacgcc cgtactacgt cgcggacatc ggtgaagtcc cgtgccgccg tcagattgcc
     4081 cacacgcatc accggctccc gcttgccagc catgatctct gccacctgtt tgcagaagtc
     4141 agaaaccacg aactggggca gctgtcgggg tcccacatga ttgaatgccc gtaccatcat
     4201 cacgtgcatt tcatacgcct tggcgtagag tgccccgaac aggttctgtg ccattttggt
     4261 aatggcatag gggttcgccg gatgcaccgg tgtggtttcg ctgaccagac tggtattcgc
     4321 cggcagaatg ccgtattcct caccggaacc gatcagcaga atcttcggcg aatagtccgg
     4381 tatgtcccgc acagcgtcca gcacgttcag gcagccgtga atgttgatgt ctgccgtcag
     4441 ctggggattt ttccacgaat acgccacgga gctctgtgcc gccagatgca ggatcgcgtc
     4501 cggcttgtgc cgcaccagca gtgcttctac ggcatctttg tccagaatgt ccagattgta
     4561 cgcctcgcag ccttccactt ctaccgtttc cttcggcagc ttggtcacaa tggtttccca
     4621 gccgcagtcc tgcaacaggt gctctgccag ataccgtcct acaaatccgc cgccgccaat
     4681 gataagtgcc ttcatgttct ttgccgcctt tctgcaattt ccgccagcgt ggcatagacc
     4741 ttgtccacca cgtctgcctc ctcatattct tcttccaccc gttttgccgc tgccctgcca
     4801 tactgctccc gcagagcctt gtcatctgcc aacttctgta tcgcctgtgc cagtgccggt
     4861 acgtcttccg gcggcacggt gattcccgtc acgccgtcca gactgacatg gggcacaccg
     4921 gtaggcagag ccgtattgat gaccggcttg ccgtagacca tggcctcctg ctgcacaata
     4981 ccaaatgctt cgctgtttgc cgtggagggc agcacgaaga tgtcacagtc cgcaaaggct
     5041 gcccgcagat cggcttcttc cagattcccg aggaaatgca cctgtgccgc catgtgggcg
     5101 gcctctgtga tctggtgcag cttttcttcc atttccggcg tgccgtgccc cacgaggaac
     5161 agctcgcagc cctgcacctg tgcaaacgca tgcagcagaa cctcgatgcc cttatagtag
     5221 acgaaccgtc ccacaaacag cacccgcacc gtttccggat cctgctggat ctgccgcaga
     5281 atgggcttgc gttccacact gcggtacgcc tgcacgtcga tgccataggg aatgatacgg
     5341 cacttgtccg cgaactgggt cagaaacgga gaactggtaa tgtgtccctc cgttgccgca
     5401 atgatgcagt ctgcacggcg gagaaaccgc atcagcagcg gcttatagaa ctggagcagc
     5461 gttttctgcc gcaccacatc gctgtgccat gcgatcacaa caccgccgcg atagcccgac
     5521 agcagacacg ccagatctgc cagcggaaac ggtacgtgga attccaccac gtctgcccac
     5581 ttcgccagct tccggaactg ccgcaaaaaa gaaaaggaaa tgggacagga aaagtatgtg
     5641 cccaagctgg acgcatagtg caccggaaca ccctctacaa tgcgatcctc gcccctgccc
     5701 ttttcctgac aaaccagcac ctgcacttcc gcacgctttt gcagttctct ggaaaatgtc
     5761 cgcaccagac tttcaattcc gccgatatgg ggataatatg ctttatttac ctgcaaaatc
     5821 cgcagttttt tctgctcact cacggcaagc ctcctgatac accgcgtaca gctgttccgc
     5881 agaacgctcc cacgtgaact gtgccgcacg tttcggacca gcttcccgca gcttctgccg
     5941 cagggcttcg tcggtgtaca gctgttccag accggcggca atgctctccg gcgaataggg
     6001 gtctgccaca acggcatctt ctcccactac ctccggcagc gatgccgcat gggtagtcag
     6061 caccggcaca ccgcatgcca ttgcctccag cggcggcaga ccaaagccct cgtagatcga
     6121 cgggaacaca aacgccagtg ccccgcacat cagcgggcag atctcctcgt ccggcacata
     6181 ctccgtgaac ttcaccagat cacccagccc cagttcttcc acctttttgt agatgccgct
     6241 gtccagccag cccttgccgc cggcaagcac cagatacggc ggggcagtct tgccctgacg
     6301 gaactggtca taggcagcaa tcaaccgttc cagattcttt cgcggctcca cggtacccag
     6361 atagaggaaa tattcccgat caatgccgta tttgtccctg accgcctgta tcgtttccgc
     6421 atcttccaca gggtggaagc gttcggtgtt cacgccgcag tataccaccc gaatcttgtc
     6481 cgcaaactgc gggaaatagc gaatgatctc ctgcttggaa aattccgaat ccgtcacaat
     6541 gcgggtggca cgcttcatgg attttttcag tccggtatcc agcatatacc gcgttctgcc
     6601 gcgaaccgtt tccggaaacg tcttgtacac catgtcgtgg acagtgacca cggtctttcc
     6661 tgccaccttg ggcggaacga tatagttgaa aaagtgggtg acttccgcct tgtccccgaa
     6721 gtaatgccga taggatatgg gcagaaagtt ggacaccagc cgatagacat agccggacgc
     6781 tttcgcccag tggggctgca cattttcccg cagatagggc tggagccgct ggatcttgat
     6841 ctcctcattc ttgcgggaga aaaattgcag caaaaattcg tcgtcagggt acatcttgga
     6901 cagagcctga atctgccccg cttcacaata gccaattccc gtcatgcggc agccgatcag
     6961 cggcagtaca tcaaatgcaa tgtgcactcc gtattcctcc ttcagaaccg gtgggcatag
     7021 ccgaccgcac cggttttttc cggcagcact gcaccaagtc tgtgcagtgt ttcctttagt
     7081 tcatgatatg gaaacaaggg cagagaaact ctgcccctgt ttgattggtt tcgataatat
     7141 cgtttatcct aaggaaacac tgaataaagc ccgcctgacg gctttgcacg caatctgcgt
     7201 gcagattttc cgtcatattg cccagaaatc ccttgccaga catacagtct gcctgcggaa
     7261 tttctatgca atctgccgaa aaatctgact acgcatctta cgcacaagct ttatgcagtg
     7321 tttccctaat gacacgctta tacgttctgg cacttcagtt cgtgttctac cagagccaga
     7381 tcgttcttta ccatacgctc taccagatcg gagaaagaga tctcacgctt ccagcccaga
     7441 acagattctg cctttgccgg attacccagc agaacgtcta cttctgccgg acggaagaac
     7501 ttcttgttga cggaaacgat ggtcttgccg gtagccttgt cgataccgat ctcgtctacg
     7561 ccgctgccct gccactctac agtgatgcct gcgtgtgcga aagcagtttc tacgaattcc
     7621 cgaacggtgc gggtttcgtt ggtagcgatg acatagtcgt ccggcttgtc ctgctgcaac
     7681 atcagccaca tcgcacgaac ataatccttg gagtgacccc agtcacgctt ggaatccatg
     7741 ttgcccagtt ctacatgatc cagcacgccg aacttgattc ttgcaacagc gtctgtgatc
     7801 ttacgggtaa cgaattccag accgcggcgt tccgattcat ggttgaacag gataccggag
     7861 catgcgaaca gaccatagct ttcacgatag ttcttggtga tccagtgacc atacagcttt
     7921 gcaacgccgt acgggcttct cggatagaac ggtgtctttt cggtctgcgg catttcctgt
     7981 accagaccga acatttcaga agtggaagcc tgatagaagc ggcattccgg ctttaccgaa
     8041 cggatcgctt ccagcatgtt ggtaacgccc agagcgtcga tctctgcggt agccagcggc
     8101 tgttcccagc tggttgctac gaaagactgt gcagccaggt tgtacacttc gtctgcctga
     8161 gagatccgca ttgcggagat cagggagatc acgtccgtca tatctgcata gatgaagtgg
     8221 atcttgtcct tgatgtgctc tacgttgccg taatccacaa cgctcttacg acgcatgatg
     8281 ccgtatactt catagccctg ctccagcagc agttctgcca gataggagcc gtcctgtcct
     8341 gtgatacctg taatcagtgc atgtttgctc atagtaattt cttttccttt ctatatatca
     8401 gaaatcgtcg gttttgcttt tgacgatttc gatttatggc aataccgcac aaagattgtg
     8461 cgacagatgc gtcgtcaaat ttttcg
//
LOCUS       DAWBGR010000106         8447 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2280_length_8447_cov_2.848785, whole genome shotgun sequence.
ACCESSION   DAWBGR010000106 DAWBGR010000000
VERSION     DAWBGR010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8447)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8447
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2280_length_8447_cov_2.848785"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3338..3347
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6011..6020
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2724 a         1650 c         1885 g         2168 t
ORIGIN      
        1 acattacaca gcttggtaca atgaacgatc tgctgattgg ctatctcgac caaagcgata
       61 ccagccaatt tcttgataac ggcagacaac ttgatttgct tctatttgca aaacaggagt
      121 ttgacttgca aattgcacag cgtgaggaac tggtatctgc tgaagaaatt gaccttccgg
      181 agccggattc tgtatcgctt cgctttggcg ataaagatca agattggttt acggaaagtg
      241 atctgcttgc cgattttgtc gatgaaaatc ctgaaatgtc atttgccctt gccaatgcag
      301 tacttggcta tcttgatgaa aagcagcata cagaacgtga tattgctgac ttgaaggcag
      361 gctggtacaa gaaaacggat tttacggtta ccaccatgat tgacggacag gaattccgtc
      421 attccggacg ttttgacatt ggggacggtg taggaacagg cggcggaacg ttgatcgacc
      481 atattgaaca gtttgcgaag aatgcattag aacaggagtt ttcttataat gaaaacgatc
      541 gtgaatcact gaattatctt cttgaccgtg ttgtgccttt cttagcagaa aatgctgcac
      601 ttacagcaaa agaacaacac attttaagtg atttcaagat acagcacccg attcgtacag
      661 ctgctgatgt ggaaattact gcatcggttc cagctgaaaa agaagttgaa ccgcctgcca
      721 ttgcagattt cagagcaaaa acagaagaac attttaagtt ctataacttc aaaccagttg
      781 agattgaaaa tatggtcaag ggttttgcac agagaatctt tgaggaaaac ggtataaatg
      841 cagaggttca cggtgcagct gttgccggtt ctcgctcccg tgggcttgaa aatgcggatt
      901 cagatattga tgttgttctt gaagtggatt ctgacttaaa ggaagatgct cttttcaata
      961 tcattcatga agaagcactt acactggaag gatatacaat tgacatcaat cccatcaaag
     1021 cggatgaaac aggtacactg gaaacttatt tacctacagc agaagcctat cttgctgaca
     1081 aagcacaatc tacagaactg tctgatttgg atattgcaaa acagcacatt gccgactact
     1141 acgaccgaga attcggtgaa ttagctgagt tttctgatct ttcaaatatc tcgctcgctt
     1201 atacaacaga cgaagaacat aatctcccca tacaggtgta tgcagatctg gaacaataca
     1261 ggctcatatt tgagtatgat ggcaatgtca ttcgtgatga gcagtataac accctttcag
     1321 agatgaatga aaatgctctg tcagtacttg attttaatga tttggtatcg ctttctgatg
     1381 atgagatcgc atcagttatt ccaacaaaga cttctgattt gccgttcaaa gttggcgatg
     1441 atattgaatt tcagggaaaa gagtggcata tcgacagcat caatgaggac agaggaagaa
     1501 tcatgctctc acgagatacc ggcaatgtca tcatgccaca agagggactg gaaacgttct
     1561 tgtctgaggt tgttgaatct gccaatcgtc atgcaggtat tgagaatttg gaacaaagtg
     1621 ataccgaaaa agtgcatgaa agtattgtag atacagatac acttgatacg gctgacaagt
     1681 ctattgcaaa tggaaaagag cctgaacaga aatctggttt acttactttc gatgtatata
     1741 tctatgatag agaaagcggt acaaattcac cgatgcgttg tcaggcaaaa aatacagcag
     1801 aagcgaaaaa gatagctgat gaatatatca atcgttggaa tctggtcgat gcggagatta
     1861 ctgatgttca gcttgtagca ccggaaaaag aacttgtgaa agatgaaccg attaccgaaa
     1921 aatcgtctga aactgaaatt tctgatgatg caccgctgtt tgacgaaaat gccattgccg
     1981 atgcagggct tggatttgta gcggatgaca aggagttcaa accttttgga aatggaaaac
     2041 aggaatatga gcagctgact ctgttcggag aatcagaacc tgtaaactcg tccacatctg
     2101 cacaagccat tgcacctgta cagtatggcg aacctgtcgc agatgtcaac cgttttgaag
     2161 aactgcacaa ggagatcatg cgtggaagcg gatttgaggg cggcaaattt cgcattgcag
     2221 aattttttca agaaaatagc ccaagcaatc aggaatttgc tgactttctg aaaaacgagt
     2281 atggcacagg tggtcatact gctgacggca atatcttctc cgttgaccat gactcaaaag
     2341 gcatacattt cacactccga agtgttgata atggaatcag caaggaaaca tttgatttca
     2401 gttggacgga agttgcaaaa ctcactgctg accttattca acatgataaa tatatcacgc
     2461 agaaagatat tgaccgcagg aatcgacgtg gatctaaaac agaaaaatcg gatattaagc
     2521 cggaaaatat agctgctccc gatgaaaaat tggaacagca aattaaagca gatgaaaaga
     2581 cgcagaattt caccataacc gatgataacc tcggtgaagg cggtgcaaaa accaaattca
     2641 agaacaatct gcttgccatt cagacattga agcaaattga aagtgaggac agaacggcta
     2701 ctcctgaaga acaggaaatt ctgtcaaaat acgttggctg gggcggtctt gcacaggctt
     2761 ttgattctga gaatgagcaa tggagcaaag agtttgccca actatctgac gcacttacac
     2821 cgaaagaata tgaagatgca agaaaatctg ttcttgacag ctttttcaca tctccagcaa
     2881 ttattgatgg aatctacgaa gcattggata agttcggctt caatggcggc aatctccttg
     2941 aaccgtcctg cggtgtcggc aattttctcg gtaaaatgcc atcagaaatg gctgaacata
     3001 caaatatcta tggtgttgaa attgattcta tcagcggacg aattgcaaaa cagctttatc
     3061 ccgatgctga cattcagata accggttttg aaagcaccca ttttcaaaat ggatcttttg
     3121 atgctgcggt cggtaatgtg ccatttggag atctgaattt tccggataaa cagtatggca
     3181 caaccaaact gcatgatttc ttctttgctc agactctgga caaggtgaaa gaaggcggta
     3241 ttgttgcttt tgtcacttct aaaggaacac ttgacaagaa agacgaaagt ttccgtatac
     3301 agctggcaga gaaagccgat ttaatcggtg cgattcgnnn nnnnnnngaa caatgctttt
     3361 aagtcgaatg caggtacaga agttacttcg gatattatct tcttacaaaa acgctccgca
     3421 ccgtcggaag aactgcctga ttgggtgcat ttaggtaaaa ctgaggacgg actcagagta
     3481 aacagctact ttgcggaaaa tcctgaaatg gtacttggta cgatcgtaga aggcaacaag
     3541 ctttacggca gaaatgatga tacaatgtgt attccgattg aaggtgcaga tcttcgacag
     3601 cagattcatg aagcggtgca gaaactgaat gcacagattt ctcaggtgaa aaccaatgca
     3661 gttttccaat tcagcgacaa gcaggttaca atgactgctg ctgaacttcg tccgttcagc
     3721 tttttcaaag atgataaggg cgatatttat ctcaaaggcg gcgaaagcaa gcttgggaat
     3781 tttgatgttt ccaaagtaat ggatacaaaa tcgaaagact acgaccgtat gactgcattc
     3841 attgatttgc gagatatggt tcgtgaactt ctcaaagtac aggcagacga ccgtcctgaa
     3901 aatgagatta aggcaattca gcaaaaactc tctgttcagt atgatgattt ttacaagaag
     3961 tatgggcttt tgcacagccg taaaaaccgt caggttttgc gtgatgattg ttcctataac
     4021 ctgttgctca ccctggaaaa ggaatttgac aaggacaagc tgatcgcaaa atccgatatt
     4081 tttaccaaga gaacggtaag accatcaaaa ccgattgaac acgtagatac agcactggaa
     4141 gcacttgcga tctctatggc ggaaactgca aaagttgacc ttgcctatat gtcaaaactg
     4201 acggatatgt cagaagaaat gttaatttca gaacttaaag gcgagatttt tcttgtacct
     4261 gataccaaca cctatcagtc ggcaagcgaa tatctttcag gtgatattcg tcagaaactt
     4321 gacattgctg aaaactatgc agaaaaagac agcagatttg ctgaaaatgt ggaggcactg
     4381 caagctgcca tgcctgaacc gctgaaagcc ggtgatattg atgtaaaaat tggtgctgct
     4441 tggattccga aagaactgta tcagcagttc atgtatgagc tttttcagac accgagagaa
     4501 aaccgtgagg acgtaaagaa agcgttctgg gtgaaactga aaaatataac agtggattat
     4561 tccgaacaca ccaacacctg gcatattgag aacaagaggg cggacagttc cgttttgagt
     4621 cgcagggatt ttggaacaaa gaatatgagt gcttatgaga ttatggagca gcttctgaat
     4681 ctgaaagagc caaaggtgta taagtcggaa acctatctgg atctgaatgg caatgaaaaa
     4741 gaacgcagag tggtcgatat caaggcaacg aagatcgttc agcagaaagc tgcaaagatc
     4801 aaacaggctt tcaaggattg gatatttaga gatccggaac gtgctattcc ccttgtagaa
     4861 cagtacaacc gacaattcaa ttctattcgt cctcgtgaat atgacggctc agctttaaga
     4921 tttccgggta tgaactctgc aatcacgctg aaagaacatc agaaaaacgc tattgcacac
     4981 gctttatttg gcggcaacac gctgttcgca cactgtgttg gtgcaggcaa gacgtttgag
     5041 atgatcgcaa cagcaatgga atccaaacgt ctgggcttat gcacaaagcc gttatttgcc
     5101 gttccaaacc acctgacaga acagattggt gaagattttc agaagctcta tccgggatca
     5161 aatattctgg tagcaaccaa aagcgacttc aaaaaggaaa accgacagca gctgtttgca
     5221 aaaatcgcta ccggtgattt tgatgctgtg attatcggtc actcacagct tggcatgatt
     5281 ccactttcca aggaaagaca ggtatctatt ttacaggacc agatcaatga tattatggca
     5341 ggcattgaag aactgaaaaa ggcagaaggt tctcgttttc aggtcaagca gatggagcgt
     5401 accaagaaat cgctggaagc aaaactggat aagctggaga aaacacatga tgatattctc
     5461 acctttgaag aaatgggcat agacaagctg atcatcgatg aagcccacga gttcaaaaat
     5521 gttcctactc aaacgaaact gaccaatgtg gcaggaattt cgtcctctgc ttcacaaaaa
     5581 gcacttgacc tctttatgaa atgccgttat cttgatgaaa aaaccggcag cagaggtgtg
     5641 attatggcta caggcacgcc cctcagcaac tctgtaaccg aactacatac catgatgcgt
     5701 tttttggagt atgacttcct gaaagatcat ggacttagta attttgacaa ctgggtaacc
     5761 gtattcgggg aacagaaaac ggaatgggaa cttgctcctg ccggcaacaa attcaaggaa
     5821 cgcacccgaa tcgccaacta tacaggtttg ccggaactga tgagtatgtt caaacaggtt
     5881 gctgatgtgc gtacagcaga cacgctcgac cttgctacgc cggaaatgaa gtatcacatt
     5941 gagaacatcg aagcaacaga ttttcagaaa acacttgtac aggaattatc cgaccgtgct
     6001 gatgatgtac nnnnnnnnnn gtggataaca tgctccgtat tacttctgac ggcagaaagc
     6061 tgggacttga cccaagactg atcgacccgt catttgagga tagccctgat acaaagctga
     6121 atcagtgtgt cagaaacgtt acgcagatct atcacgatac agcagaggaa aagctgaccc
     6181 agatcatctt ctgcgatttg ggtgttccaa agaaaaactc acagtcaacg gataagggag
     6241 aaaatgctga tgaaaagtca gcttctgaaa gagaatcttt agaggaagaa tgtgatttct
     6301 gcgtctatga tgatattcgg gataagctga tcgtaaacgg cataaaaccg gatgagatcg
     6361 cctatgtaca tgatgcaaaa accgaacagc agaaagctga tctcttcgac cgtgtgaata
     6421 aaggtgaagt gagagttctg ctgggcagta caggcaaaat gggtacgggt acaaactgtc
     6481 agaaaaagct gattgcattg cataatctgg atattccttg gcgaccgagt gacctgcaac
     6541 agcgaatcgg acgtatcgaa cgtcaaggca accagaacag cgtggctcat gtctttaact
     6601 atgtgacaaa aggcagtttt gatgcgtatc tctatcagac actggaagca aagcagcgtt
     6661 tcatcggaca gatcatgact tccaaaacgc ctgcaagaac ttgtgaggac gtagatcagc
     6721 aggcactcaa ttattctgag atcaaggcac tctgtacagg agatgaacgt atcaaggaaa
     6781 agatgatgct ggataacgag gtaaaggaac tgaatcttct gaaagcggaa cataccaata
     6841 cggtatttga tatgcagcag acagtagctg tcactcctgc aaaaattgaa aatgctgaaa
     6901 taagacttga aaatctcaaa gctgaccgtg aaacacttcg tcagcttccg atagatgaag
     6961 aaacaaaact acccgtattc aaaattacca ttgatggcac ggaatatacg gaccgtaaag
     7021 aagcagcaca ggctcttgaa gatacagtac tggattttgt caaaaagagt tccgacagtg
     7081 aaaaggaaat cggtgaattt cagggattca agctatccgt caaggcggtg atgtttggca
     7141 atgaacctga catttccatt accatgaaag gcaacgctga gcataaatgt gctttaacaa
     7201 gcagcttttc tgccaatctc aagcgtatgg aatctacact ctacaaaatt gaccagacga
     7261 tcagtgatgc acagaacaac ataaataagc tgaaaatgaa tttggaaaat gctatgcgta
     7321 ttgtcaatac accatttcct cagcaggcag aacttgaaga aaaaacagaa cggcttactg
     7381 ctcttaccga agaacttaat caggcagcga tggaagccaa gaaaaatgct cctgaaaaac
     7441 agcgtacctg ctattttgaa agggctaagc tgaaaaagga agcctttaag gacagacaga
     7501 aaacgactaa gccgaaagag agaagtcagg acaaaaacaa aatagaatga taaaacaggc
     7561 acttcaattt ttacgaagtg cctgctttcg ttaattcata tattgctgta cacagctttc
     7621 atagagaact tttgtgtatc gctctttcag tctgatcgga cttatgatct ccacctttcc
     7681 cctgaatgaa aatatccagg aaaagaacgg ctgcggatat tctgctctta caggaacatg
     7741 aatgataaag tgattgtccg atttcttgtc cctgagcatg agaactgact tcccgaaccg
     7801 gtcgatcacg atgttacaat atttattttc cagcggcaag cggagtgtaa cgtactcact
     7861 ttttccgctg aacatagaaa aagatgattt gatgtactgt tccagattca atatttcaca
     7921 gggagtattt ctgtataaat cgataagttt gatgtttctc attctgtcaa tacgaaagtt
     7981 cgtataggta tcaccatgtt tttcatagtt ggatacaaca tagtactgtt cattacacat
     8041 caccaatgca aacggcgaac aggttcgttt tactccatct ctgagaacta attgcttatt
     8101 tatatcatag tcataatagt caaagctgat ttgctgatta ttatgtaagg cagaatttat
     8161 catatcaata ttgtcaaaaa tctgctcgtt atcagacttc acacgattgg tcagcataat
     8221 ttcacgtctg agtagtggag cttcataaat actgcaaaga ctttccagca gtttaagcaa
     8281 tttttctgaa tccttaactg ttaaaaagcg aaatgaagca actgcatcag cgagcatttt
     8341 aagaacactg agcgaaaatg ttcggctttt caaatgatac cctttattgt aaatgtattc
     8401 aatgtcgtat cccatatata tcagtgcatc aatatcttct gtgatag
//
LOCUS       DAWBGR010000107         8403 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2294_length_8403_cov_4.693699, whole genome shotgun sequence.
ACCESSION   DAWBGR010000107 DAWBGR010000000
VERSION     DAWBGR010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8403)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2294_length_8403_cov_4.693699"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2070 a         2109 c         2284 g         1940 t
ORIGIN      
        1 cccctccacc agcttcgctg gccccccccc cctttcaggg gaggcttata cataagtccg
       61 cggggcgatg tgggcatcgc cccctacacg ttccaagaga tttctccgta aaatccaaca
      121 cattaagaaa aggtttcccc tcagcagcac tgtccttgtt tcacgtgaaa cattctgtgt
      181 gacacgctta tccccgcagc tggatcggca tcagcaggaa aatgaagctt tcgccctgcg
      241 gcggaaccag ttttgccacg cgattgccgc cgctcagctg gatttttacc ttgtcgcagt
      301 ctgccgcacg gacagcgtcc aacagaaaac ggttatcaaa accgatggtg acatctttgc
      361 cgctgatctc caccggaatt ttgtcgttga tctcaccaat accggtcttg caccacacgc
      421 caagactgcc gttggcaaac gtgcagcgaa ccggtgcgtt gtacttaccg ctgatcagca
      481 gggaacagcg ttccagacaa cgggtcaggg cactggtgtc cacagtgatc tcagtttcat
      541 agctggcagg aatgctgctg cggtagttgt ggaactcgcc ctccagcagg cgggagaata
      601 ccgtatagcc gttgaattcg aataccacgt gccgccgatc cgcagaaatg gtgcagagtt
      661 cctctgcgtc atcgtggagc atgtttgcca cttccagcag catccgtttg ggaaccacaa
      721 aacggtaatt ctccgcactg gaaatgggtt cctgccggac tgccagccgg tagccgtcaa
      781 tggctgccac atgaagggta tcgccctcca cctcaaacaa ttcgccggtg agcactggtt
      841 tcgcctcgct gacagaagtt gcataacttg tctggcgaat catggaattc aataccggct
      901 gttcgatctg catgccgacg ttcagatcca gactgggcag agccggatat tcctcagagg
      961 acatggcgga gatctgaaac tcggtggcat cacagaacag ttttaccaca tagttgtcat
     1021 ccacttccat ggttaccata ttgccggaga accggcggac agcctcactc agcagacggg
     1081 gaaggatcac aaattcgccg gagcagtctg attcgacttc aatgctccgc tggatcccga
     1141 attccagatc atagccggtc agttccagat tgtcgtgatc cagccgcagc ttcaggcgtt
     1201 ccagtgccgg aatggtggat ttttgagaaa caccctttgc cacatgggta atggcagtat
     1261 agaatgtttg tcggtcacaa gtaaatttta tcatgggtcg tcttccttta ttcagatcca
     1321 atggatcggt caattttgaa tggggttcgg aacgtgtgtc ccggacaacg ctgtatccgt
     1381 tttgtatctg tgttgtccga tgtctttccg attgtccttt tgcagccact tcgcggacag
     1441 aaaatattac gcagttcagc cgtttttctt gtttttgcag ctgtagcaag aacagttttt
     1501 tgctgttcgt ccacagtgta cagcacatgc tatccgctgt tttttgcagc gaagctgcga
     1561 aaagacagaa tacaaagaaa agaatatata gtagtaataa tagaggctcc ggaaaagtgg
     1621 aaaccgcaga ttctcctcgg aatttcgggc agatctttcc cacagaccgc ctgtggaaag
     1681 tttccggaaa agtggaaagc tttttccaca cgggaaaagt ggaaagtttg tggagtggaa
     1741 aacaaaagtt tgtggaaaaa atcgtggaaa acctgccgca ctcgaaatgc cgcttccaca
     1801 agctgtggaa aacacattcc acagctttct accacagatt ccacaagccc gcttctatag
     1861 ctgttaaatg ctgcggacac agcgggcact ttttctgcac atggattttc aacagactgt
     1921 tgaaacgcat gtggaaaact tgccgacgcc ggtggaattc ctgtggaatc ggcaaaaaag
     1981 cactgcaaaa tcctgttttc cacatgttct gcagtgtcct ttcccagttc tctatgcgtg
     2041 gcttttgttg cggatatttt tgatggtgtc ctcgaccagc tgcttcaggt ggtcatcgtg
     2101 cttcattgcc gtttcaatgt tgctgatgga gtagacaatg gtggagtggt cacggccgcc
     2161 gaattccgtg ccgatcgcct gcaggggcat ctgggtgatc tcacggacca catagatcgc
     2221 caccttgcgg gcagtggaga tctggctgga gcgtttgctg ctgcgaatgt cctccggaga
     2281 aacgccgtac acgtcagcca cttccagaat gatcttctcc acggtgatcg gagccggctg
     2341 gtcgtcgctg agaatgtcct tgatgacgct ctgtgccatg gaaatggacg ggggcgaacc
     2401 tgccagatgc ttcagggctt tcagcttctt gacggcaccc tccagctgcc ggatgttggt
     2461 tttcagacgg ttggcaataa actctgccac atcgtcggga atatacagat ccagcagttc
     2521 tgccttgcgg cggataatgg cgatacgggt ttcaaagtcc ggtgtgctga tgtcggtgat
     2581 caggccccac tcgaaccggc tgcggatccg ttcttccagc gttttcagtt ccttgggcgg
     2641 cttatccgaa gtgatcacga tctgcttgcc ctcagcgtgg agcttgttga acgtgtggaa
     2701 gaattcctcc tgcatgcgtt ccttgttgct gaaaaactga atgtcgtcga tcagcagcac
     2761 gtcggcgttc cggtacttgt cgtggaaccg agccatgttg ttgtcgttga tgcaggagag
     2821 gaagtcgttg ccgaattctt cactggtgac gtaaatgatc ttgtattcag ggtgattttc
     2881 gtgcacctgg ttggcaatgg catgcatcag gtgggtcttt cccagaccgg agggaccgta
     2941 gatgaacagg gggttgtagt tcttgcccgg ttcttttgcc acggcggtgc aggcagcgta
     3001 ggcgaactcg ttggagccgc cgacaatgaa tgtggcgaag gtgtactcat agttggcacc
     3061 ctcaaagctg tgctccagtt cctcgtggcg gcgtgccagt tcatcgtagt ccttggcgtt
     3121 gctgttttcg gaaagcgggt tgtcattttc ttccggtgtg acgacattga tctggatctt
     3181 gacatccagt cccagcacgt tgagaaatgc ctctttcagc agggcattgt aggtgttcat
     3241 aacaacggtt ttctggaact ctgactgtac ggtgaacact gccaccgagc cgtccagctg
     3301 taccggcttc ataatatcga tccagagctt catggcagcc tctgagatcc ggccctcttt
     3361 ccggcagtac tgtttgacac tggagaaggc ttcatcaatt gaattcataa atgggggatc
     3421 ctccttatgt aacttcaaag cctgtccggc aggatctgcg gggatctccg tggtctgccg
     3481 gacaggcgaa ttctgcggaa cggtggttgt ggaaaaccgt tttccgcaga ctttccacct
     3541 gtctttcaac aggtattctt aatataatag tataccacat catctgtgga aaatcaaccc
     3601 ctattgtgga aaactttatg aattccacaa aatttccacg tggaaacctg aggaaactgt
     3661 ggagagcaaa aacggacaaa aaacgggcgt tttccacagg cggtgcagcg tggaaagttg
     3721 ccagacgctc tttgtgcggt attgccttca ggcagccaac ggcaaaacgc gggtaccgga
     3781 gccggctgcc gcgttgccgg aatgccgtcc tgctccggaa tgctggggca gccggcggaa
     3841 ttgtctttgt aaaaaacgtc aaaaaccgac aaaaaatccg tgcagcctgc ccaaaacagc
     3901 tgtggagaac ctgtggaaaa agaaaaaaag ccgaaaattt cgcaaaaaag tttgccggag
     3961 ggcttgacaa ataaaacgga atccgctata atatatggta aatggattca cgcagacagg
     4021 ctcatccggt ctgggtggac ttccaaggaa tctgtgggac ggcagttctg ccgtctgagc
     4081 gagaaaggag gtactgtttt gaagagaaca tatcagccga agaagattca tcggaaaaag
     4141 gtgcatggct tccgcaagag aatggccact gcgaacggta gaaaggtttt agctcgcaga
     4201 agagcacggg gcagagcaag actgagccac tgatcgaagt cggaccacaa cgccattggt
     4261 ttgtggtctt ttgttttttc gaggagcgta ttctgaattc gccgatgtgc cggctgcgga
     4321 aaactgaccc ataccgccat ggtacaaggt cagacagtcc gcagccggca cacggaagat
     4381 tcggagtacg cttttgcttt ttccggtaaa aaccgtcaac aggctctgaa ggaaacactg
     4441 cataaagctt gtgcgtaaga tgcatcgtca gattttcggc agatggcgta caaagccgtc
     4501 aggcgggctt tatgcagtgt ttcctgaaca ccaaaaaatg gagtgcaagc aatggagaaa
     4561 acagtgatcc tcaaccagaa tcgggacttt cagctgctgt atcagcgggg aaaaaagatc
     4621 gcatcgccgt atgtgatcat ttatctgcga aagaccagac gaaagtataa ccggctgggc
     4681 attaccgtag ggaaaaaagt cgggtgtgcc gtaaaacgca gccgtgcccg ccgtgtgatc
     4741 cggcaggcat atcgggaaac cgaacacctg atgccggtgg gaatggacat ggtcattgtg
     4801 gcgttgccct ctattgtggg cattcgctcc acacaactgc gtgactatct ggcaggaagc
     4861 ggcgttcgcc gtatccgtaa actggtggaa ccgccgtcgg aagggggaaa accgaaatga
     4921 ggtatctggc ggcggcactg atccgtctgt accagaaatt ctcccgccta actccggctg
     4981 tgtgccggtt ttatccgacc tgttcctgtt atgcacagac tgcaattttg cggtttggtg
     5041 tgctgcgggg cggctttctc gccctgcggc gtctgctgca ctgtcacccg tggaatcccg
     5101 gcggtatcga ttacgttccg gaacatctgg atccgccgcg ggaagtcttc gggcggtatg
     5161 tgcgggcaat acgacacaac agaaagggtt aaacaatttt atgagtgatt ttttatatga
     5221 tctgtgcgga aagccgttcg gctggatcat gcgtctgatc tacgacttgg tagacaacta
     5281 tgctttggcg atcctgttgt tcacgatctt caccaagctg atcctgtttc ccatttcgta
     5341 taagcagcag aaaaattccg caaaaatgca gcggttcaat ccgaagctgg aaaagctgcg
     5401 gaaaaagtat aaggacgacc ccaagaagat gcaggaagaa cagatgaaac tgtatcagga
     5461 ggaaaacatc aaccccggtg cttcctgcct gcccatgttc ctccagttct ttatcctgtt
     5521 cggcattctg gacgttgtat ataagccgct gtcctttatc ctcaagatga aagacagcgt
     5581 gatctccagt gcttttgaga tcgtccggaa tgtcaatccg gaactcgcca agcagggcac
     5641 cagcctccgg cgggaactgg cgatcctgag tgcacagcac agcgatgcca gtgcattcca
     5701 gagcgtgctg ggggacaacc tctccgcaaa gatgaccgaa ttctatgaca agttcaacct
     5761 gttcggcatc aatctgggcg aagtgccgtc gtggcaccct gacgtgtgga acgctaccgc
     5821 gatcggcttg ttcctgattc cggtgctgtc cggcgtgctc cagctggcac tgaccattta
     5881 tatgcagatc taccagaaga aaaagaatcc cggcatgccg aatatgggct gcatgaacat
     5941 catgctctat gcaatgccga tcttctccgt ctggttcgca tttcaggtgc cggcaggtgt
     6001 cggcttctac tgggtatgct cgtccttctt ctccctgatc cagagcgttg gtctgaactg
     6061 ctacttcacc aaggagcgta tcgaggcgat ctgcgaaaag gaaaaccaga agaacgccaa
     6121 gaaatacgcc aacggcaaaa agagcttcat gcagcgtatg atggacgtac agcagggcaa
     6181 tgacgtcatg aaccagcggg aaaaatatgt agaagaaacc aaggatatgt cccgttcgga
     6241 actgaacaag tataaccagc aggtgctgaa agacgcacgg aaacgcatgg cagaaaagta
     6301 cggcgaaacg tatgacgaat ccgacagcac cgaggacagc ggcgatcaga aataatcgtc
     6361 cgtgaaaaat caattaccac agttcacagg gaaccgccct gtgaaatccc attaccaagg
     6421 aaagggatga atgagaatga aagagatttt cactgcaaaa agcatcgaag aagcaaagca
     6481 tctggcggtc gaaaagttcg gcgtttctga agaccagatc gtgttccgca ttctggaaga
     6541 accgaaaaaa ggaattttcg gaaaggtaaa gcgggaagca caggtagaag cagtgtacga
     6601 agcagcagaa gaagctgcac cggcagaaac cacagaagcc aaggctccgg aacagtcgga
     6661 agccccgcag ccgcaggcag aagccgcacc ggcaatgccg gtttcccgac cggtacagga
     6721 agcggtttct tccgtggagg agatcattat tgacaaggac gacaattcgc ccacacgggg
     6781 catggagtcc gaagcgatcg aaaccattct gaccgagggc gaaatgtccc cgaacctgct
     6841 ccgtgcaaga cagtatatcg cagacatcta cgctgccatg ggcatcaccg tgaccatcag
     6901 caccatgcgt accgcaaacg gcattcgcat ggaacttcgc agcgataata aaagcggcac
     6961 gatcatcggc agacgaggcg aaacactgga ttccattcag tatctggcat cgattctggt
     7021 gaataaggac agcgaggatt actgccgcct gctgctggac agcaacggct atcgggaaaa
     7081 gcgtcgcaag acactggaac agctggcaga aaagatcgca cggagcgtgc tccgcagcgg
     7141 tcgttccacc acgctggaac ccatgaatcc ctatgaacgc cgcatcattc acagcaagat
     7201 ctcggagatc gagggcgtga cttccaagag cgtcggcgaa gatccgtacc ggaaggtcgt
     7261 agtttcttct accaccggca gacgcagcgg cggaaaccgc ggcggcaagg gcggtcagaa
     7321 ccgcgggggc aagcgtccct atcgggaaaa gaagccggaa gatttccgcc ggaagaatct
     7381 ggattccatg aagaccagct tcgaacggga ttataaaaag ccgaagccgg aggacgaact
     7441 taacaccggc ttgtacggca agatcgaatt ctgatcggaa actgaacgca gatcacagaa
     7501 cggattgtag caatgcagtc cgttttgttg tatctgtttg gaatgtgtag aaggcgatgc
     7561 ccacattgcc ccgcggactt atgcataagc ctcccctgaa aggggagggg gaccagcgaa
     7621 gctggtggag gggttcttcc gtgcagtata acttctcatg caaccgtgtc gaaaccgtcc
     7681 ggcggaattc gctcgttgca ctcgctgtcc gcctggggct tgccaacgcc gattgttgcc
     7741 cttgcttgta tttgcagtgt tggttttttg aggtgttgct tgtgcaagcg gtcaagtttc
     7801 ttaaatacgg cgtttgcaag cttacataca gcctcccctg aaaggggtga cttcccacgg
     7861 ggtgaggaga tgcccgcagg gcagagggga atgctcagta cgaggggaac cgccgttagg
     7921 cggtggaggg gttcacccgt aaagtgtact ttctagtgca gccacaccga aaccgtccgg
     7981 cggaattcgc tcgttgcact cgctgtccgc ctggggcttg ccaacgccgc tagttgccct
     8041 tgcctgtaca tgtagtgttg atattcaagt atttttctta tgtaagtggg gtaactttat
     8101 ttcatacggc gtttgcaagc ttgtgaataa ggcttccctt tagggcaccc gaagggcgtg
     8161 ccttgtgctg agctggcaag ccgtaggctt gactgagggg tattcgctac agcagccagc
     8221 cggtttcaca aggagttacg cttctttttt tctttgctga aaagaaaaaa agaagcaaaa
     8281 aaagaaaaga gttcgcgttt cacttgtact acaaggtcaa gtatcacacg aagattacac
     8341 gccgcaaaaa tcccccggcg gggcgaacag gcggctgctg ccgccgtaac attacccttt
     8401 tta
//
LOCUS       DAWBGR010000108         8391 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2298_length_8391_cov_5.070777, whole genome shotgun sequence.
ACCESSION   DAWBGR010000108 DAWBGR010000000
VERSION     DAWBGR010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8391)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2298_length_8391_cov_5.070777"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1771 a         2267 c         2225 g         2128 t
ORIGIN      
        1 tgacgaaaaa tttgacgacg catctgtcgc acaatctttg tgcggtattg cctttttatt
       61 ttgttttgtc tgcggaattg ccgtcagatg ccgcctgttc cttggcagcc ttcagctttt
      121 tcaaactggg acgcatgacc agaaatgcag tcacgccgca tgccactgtg aaaatgacca
      181 gcgtttgcac gatctgtctg gtatttccgc tgtcctttgc atcgtctgtg gattctgccg
      241 cagtagtctg cggggcagtg gtttctgccg ccgcagccgg aactgtcagc aggcttcctg
      301 ccagaagcag tacgcctaac aggctgcaaa gcacttgttt ccaacgccgc tgcataccgt
      361 tagctcaaag aggacggaat ggtggaaggc ggcatggagg aaccggtgtg ttccagataa
      421 gcatcattct tgaccggctt tacgttgttg tacacatcga taccagtacc agccggaatc
      481 agcttaccaa taataacgtt ttctttcaga ccatacagat agtcgctctt gccgcgaatt
      541 gccgcatcgg tcagagcctt tgtggtttcc tggaaggaag cggcagacag gaagctgtcg
      601 gagctggagg atgccttggt gataccctga agcatcggct ctacagtgac cagagccact
      661 tcggtttcgc cgttgttgat gcgttcctgc aatccgtcgt tgacttccat gatcttgcgg
      721 cggtctacca tggtgcccgg cagcagatag ctgctgcccg gatcgtctac cttgaccttg
      781 cggagcatct ggcggacgat aacttcaatg tgcttgtcgt tgatgtctac accctgcaga
      841 cggtaaacct tctgtacttc gctgatgata tagtcctgaa ccgcctgaac gcccagaacg
      901 ttcagaatat cacccgggaa gacgttgcct tcggtcatgc gggtaccctt cttgatgaat
      961 tcgccttcgc gaacacgaat cttgaagtta tagtggatcg gataggattc cgatgcgtgg
     1021 gtttcttcgt tggtgacgat aatggtgcgg gaagtcttga cttcttcgat gtgtaccaca
     1081 ccatcgaggc gggacaggat cgcagcattc ttcgggtgac ggctctcgaa cagttcttcg
     1141 acacggggaa gaccctgtgt gatgtcttct gcggaggcga taccaccggt atggaaggta
     1201 cgcatggtca actgtgtacc cggttcaccg atagactgtg cagcgataac gccgacagct
     1261 tcaccgatgg aaaccagatt gccgtgtgcg aggtttgcac catagcactt tgcacacaca
     1321 ccctgttttg cacggcacat gaggacagaa cgaatcttga tacgctctac gccggaatcc
     1381 acgatcttct gtgcatcggc ttcggtgatc atcttgttct tggagatgat aagctcgccg
     1441 gtctgcgggt cacgcagatc gtctaccaga taacgaccgg tcagacgttc tgccagcttt
     1501 tcgatcagct cgttgccgtt gcggatctcg tagatctcca gaccgtcggt tgcaccgcag
     1561 tcctgttcac gaatgataac atcctgagaa acgtctacca gacgacgggt cagataacca
     1621 gagtcggcgg tacgcagtgc ggtatctgcc agacccttac gggcaccacg ggaggagatg
     1681 aagtattcca gaatgttcag accttcacga tagttggaac ggatcggcat ttcgatggtc
     1741 gcaccggaag tgttggcaat cagaccacgc atgcctgcca gctgacggat ctgggaaata
     1801 ctaccacggg caccggagtc cgccatcatg aagatggggt tataccggtc cagaccgttc
     1861 tgcagggcag tggtaacgtc gttggtagcc ttgttccaga tctcgatgaa ccgcttggac
     1921 ttttctgcgg cggagatgta accgtattcg tattccgctg tgatgtcatc gacctgtgca
     1981 tctgctgcgt tgacgatgtc ccacttttcc ttcgggataa tcgcatcgca gacagcaact
     2041 gtgatcgcag ccttggtgga gtacttgaag ccggttgcct tgatcttatc cagcacttcc
     2101 gaagtggtgg cagtgccgtg gatcttgata cagcggtcaa tgatctgacc cagctgtttc
     2161 ttgccgacca ggaatgtaac ttccagatcg aactgcttgt cagaattggt acggtctaca
     2221 tagcccaggt tctgggggat attttcgttg aagatcaggc ggccgacggt gcagtcgatg
     2281 atcttggaaa cccgcttttc accgatgtcc ttggtgatgc ggactttgat ttttgcatga
     2341 atggaaacgt cgccttcctg ataagccatg agggcttcgt tgacatcgcg aaagaccttg
     2401 ccctcgccct tttcgccgtc cttttccatg gtcaggtagt aggaacccag taccatatcc
     2461 tgtgtcggca cagcaaccgg acggccgtca gacggcttca gcaggttgtt ggcagccagc
     2521 atcaggaagc gggcttctgc ctgtgcttcc gcagacagcg gcaggtgcac agccatctgg
     2581 tcgccgtcga agtcggcgtt gaatgcagag cataccagcg ggtgcagctt cagagcacga
     2641 ccctctacca gaaccggttc gaacgcctga atacccagac ggtgcagggt aggagcacgg
     2701 ttcagcagaa cggggtggtc atgaatgaca ttttccagag cgtcccatac ttccgcagaa
     2761 gcacggtcta ccatttttct ggcactcttg atgttggcga tgaccttggt gtctaccaga
     2821 cgcttcagga caaacggctt gaacagttcc agagccattt ccttcggcag accgcactga
     2881 tacattttca gttccggtcc gacaacgata acagaacggc cggagtagtc aacacgctta
     2941 cccagcaggt tctgacggaa acgtccctgc ttacctttca gcatgtcgga cagagatttc
     3001 agagcacggt tgttcggacc ggtgaccggt ctgccgtgtc tgccgttgtc gatgagggca
     3061 tcgacagctt cctgcagcat acgcttttcg ttgcggacga tgatttccgg agcgtccagt
     3121 tccagcatac gcttcagacg gttgttacgg ttgatcacgc ggcgatacag atcgttcagg
     3181 tctgatgtcg catatctgcc gccgtccagc atgaccatcg gacgcagatc cggcggaatg
     3241 accggtacca cgtccatgat catccattct ggacggttgc cggactgacg gaatgcttct
     3301 acgatttcca gacgcttcag cagcttgaca cgcttctgac cggaagccag cccagccagt
     3361 tcagttttca gttcagctgc cagttcgtcc agatcgatct gcttgagcag cttctgaata
     3421 gcttctgcac ccatgccggc ttcgaattcg tcgccgtact tttcccgata ggagtggtat
     3481 tccttatcgg tgagcagctg cttgggcacc agctcggtgt tgcccggatc gagtacgata
     3541 aacttggtga agtacaggac ttcttccaga cgcttctggg agatttccag catctgaccg
     3601 atacgggacg gtgtgccctt aaagtaccag atgtgggaaa ccggagcagc cagttcgatg
     3661 tgccccatac gttcacggcg aaccttggca cgggtaactt ctacgccgca gcggtcgcag
     3721 atctttccct taaaacggat cttcttgaac ttgccgcagt ggcactccca gtccttggtc
     3781 ggcccgaaga tacgctcaca gtacagaccg tcgcgttccg gcttctgggt acggtagttg
     3841 atcgtttccg gacgctcaac tgcaccgtaa gaccattccc ggatacgatc cggagaagca
     3901 agacaaatct tgatagattc aaaagtgtta aattccaaac cgttacctcc tatatatcaa
     3961 cgaggaaaca ggagaagcga acggaacctg ccgtccgctg cctgtctgtt ggtgtgtata
     4021 tcaaaatcgc tcaaaggcga gtgttcttga gaattacagc agatcatcgt cgtctgtgtc
     4081 gtcaaagctt tcgccgatgc tgaaatcgtc gcccagcaga tcgtcatcgt cgctgtagga
     4141 acttgtgtga ctgctgccgc cgatgctgtc gcccagcaga tcatcatctg tcggattgcc
     4201 atccttgaag gacatgccgg catcttccat atctctgtct gcaccttcat agtccgtaga
     4261 agttcccata aaggacggat catcatcagc tgcactgacc ggctgcggaa tgatcgcttc
     4321 ttcttcctcg aagttggact tgaggtcgat ctcgttctga tccttgtcca gagcctttac
     4381 gtccagaccg agggactgca tttccttgat gagtaccttg aaggattccg gcagacccgg
     4441 cttcggtacg ttctgtccct tgacgattgc ctcataggtg ttgacacggc ctacggtatc
     4501 gtcggacttg acagtcagga tctcctgcag ggtgtaagct gcaccgtatg cttccagtgc
     4561 ccaaacttcc atttcgccga aacgctgacc gccgaactga gccttaccgc ccagaggctg
     4621 ctgggtaacc agagcgtacg gaccaacgga acgtgcgtgg atcttatcgt caaccaggtg
     4681 gtggagtttc aggtagtaca tgtagccgac agtaacgcgg ttgtcgaact tttcaccagt
     4741 tctgccgtca tacagcgtga acttaccgtc ttcgttcatc tccagagcct tcgggttgat
     4801 gaccttctgc atcggcttca gatcgaacgg atcatcgcgg tgcgaagcat tcttttcgtt
     4861 ggcttcgcgg aagcatgcac gaatgtcatc ttcgtgtgca ccgtcgaata ccggtgtcat
     4921 gatgtgccag cccagagcct tggcagccat acccagatgc acttccagaa cctgtccgat
     4981 gttcatacgg gaaggtacgc ccagcgggtt caggacaatg tccagcggtg tgccgtccgg
     5041 caggaacggc atatcttctt ccggcagaat acgggaaacg acacccttgt tgccgtgacg
     5101 gcctgccatc ttgtcgccga cggagatctt acgcttctgt gcgatgtagc agcgaaccag
     5161 ctggtttacg cccggagaga gttcgtcgca gttttcgcgg gtgaagatct tgacatccac
     5221 gataatacca gcctgaccgt ggggtacctt cagggagttg tcgcggactt cgcgagcctt
     5281 ttcgccgaag attgcacgca gcaggcgttc ttctgcggtc agttccgttt cgcccttcgg
     5341 ggtgacctta ccaaccagaa tatcgccggc atgtacttcc gcaccgatgc ggataatacc
     5401 gttttcgtcc agatccttca gggcatcgtc gcccacgttc ggaatgtcgc gggtgatttc
     5461 ttccggtccc agcttggtgt cacggcactc gatctccagt tcttcaatgt gcacggaagt
     5521 aaagacatct tctcttacca gacgttcgtt cagcagaacg gcgtcctcgt agttgtagcc
     5581 ttcccatgtc atgaagccga tcagagcatt tttacccagc gaaatttcgc cgtctgcggt
     5641 tgccggaccg tctgccagta cctgtccctt gtgtacccgt tcgccggcat cgacgatcgg
     5701 acgctggttg acacaggtag actggttgga acgcttgtac ttctggagta catatttatg
     5761 tgtcttgtgc tcgtcgtcca ccagaacgat ctcgtctgcg gaaacggagt cgatgacacc
     5821 atcatattct gatacaacgc aaacgccgga gtctacggca gccttgtatt ccataccggt
     5881 gccgacgaac ggacgctcgg ttttcagaag cggcacagcc tgccgctgca tgttggaacc
     5941 catcagtgca cggtttgcat cgtcgttttc gaggaacggg atcatagaag ttgccacgga
     6001 aactaccatt ttcggggaaa cgtccatgaa gtcaacgcgt tctgcttcga tttccagaat
     6061 ctgatcgcgg aaacggcctg cgaccttggc atttgcaaag tgaccgtctt ctgtcagcgg
     6121 ttcgtttgcc tgtgcgacaa tgtagttatc ttccatgtcg gcagtcatat aaaccacttc
     6181 gtcggtgacg cagccggttt ccttgtccac cttgcggtac ggagcctcaa tgaagccgta
     6241 ctggttaatt cttgcgaaag ttgccagata ggagatcaga ccgatgttcg gaccttccgg
     6301 tgtttcgatc gggcacattc tgccgtagtg gctgtagtgt acgtcacgaa cctcgaatcc
     6361 ggcacggtca cgggacaaac cgcccgggcc cagtgcggac agacgacgct tatgggtcag
     6421 ttctgccagc gggttgttct gatccatgaa ctgggacagc ggagaggaac cgaagaattc
     6481 cttgatcgca gaagtcaccg gacgaatgtt gaccagtgcc tgcggggtga gcacatcgtt
     6541 atcctgtgcc tgcaggttca tgcgttcccg gatcacgcgt tccatacggg tgaagccgat
     6601 gcggaactgg ttctggagca gttcgcctac cgaacggata cggcggttgc ccagatggtc
     6661 gatgtcatcg acgttgccga tgccgtcgca caggttcagg aagtagctga tcgttgcgta
     6721 gatgtcgtcc agaatgatgt gcttcggcac cagttcatct gcacgcttgc ggatcgcggt
     6781 gcggagttcg tcttcgctgg acgcactgtc gaggatctcc cgcagaacag cgtagcgaac
     6841 cttttcgctg atgcccagtg cttcgcagtc gaaatccaca tattttgcaa tgtctaccat
     6901 gccgttgccg atgaccttgg cttccttttc aatgaagcgg atctcgtttt cgccggactc
     6961 ggtgacaaaa tattccttgg tttctgcctt gatataggct tccttgatgc cggcatcctg
     7021 aatctgcatt gcctgatcga aagtcagtgt cacgcctgca tcaaagagga tctcgccggt
     7081 cgcttcgtct gccaccggac gggtcagctc gtgacctgcc agacggctgg cgatgcccag
     7141 cttcttgttg tacttgtaac ggccgaaccg gcacagatcg taccgcttcg cgtcgaagaa
     7201 caggttgttc agatgtgtca gggagctttc caccgtaggc ggttcacccg gacgcagctt
     7261 cttgtataca tcgatcagag catcttccgt gttcttggtg gaatccttct ggataatggt
     7321 ctgggacaga cgctcgtcgt tgtcgaacat ctcggcaatt tcttcgtctg tgccggtgcc
     7381 cagagcacgg ataaatgtag tgatcgggat cttgcggttc ttgtcgatgc ggacatagac
     7441 cacatcgttg gaatccattt catattccag ccatgcaccg cggttcggaa tgacggtagc
     7501 cttaaacagg tccttaccgg tcttatcctt ttccgaagcg tagtatacgc ccggcgaacg
     7561 caccagctgg gaaacgataa cacgttctgc accgttgatg acaaaggtgc cgctttcggt
     7621 catcaggggg aagtcgccca tgaagatctc gctctccttg acttcaccgg tttccttgtt
     7681 ccagagcgtt gcagtcacac gcagcggtgc tgcataggtc acgtcgcgtt ccttgcattc
     7741 tgcaatggta tatttcgggg tgtcatcgat gcggtaatcg gtaaagttca gtaccagatt
     7801 gccgttgtag tctgtaatgg aagaaacgtc gcgaaaaact tcccgcagtc ccttttcacg
     7861 gaaccacttg tacgagttct tctgtacctc gatcaggttc ggcatttcca gaacttcgtt
     7921 gatcttaccg aagttcatac gaacgttttt cccgcactgc tttggcgtaa catgcaccat
     7981 tggcgaaaaa cctccttaac tctttgtcag acaaaaattc gcctgtttcc cttacaacct
     8041 tccgcacaca gcggaaaatc tttttctgtg atttgaaaaa aaggacttga cagacgaggt
     8101 cggaatgtgg tatactattc tgctgaaatg tgcattgaca cattacgata cattttatta
     8161 ttatataatg caagcgttct tttgtcaagg gcgttttttt atttttgtag gaatgccgct
     8221 gaagcacagg ctgttccgct tctttttcgt caacttgcac aagccggacg gaactgccgc
     8281 agacagcaaa ggcaacaccg cacaaagccc gcctgacggc tttgcacgca atctgtgtgc
     8341 agattttccg tcatattgcc cagaaatccc ttgccatacg gcagtatgcc t
//
LOCUS       DAWBGR010000109         8380 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2303_length_8380_cov_4.791832, whole genome shotgun sequence.
ACCESSION   DAWBGR010000109 DAWBGR010000000
VERSION     DAWBGR010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8380)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8380)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8380
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2303_length_8380_cov_4.791832"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2192 a         2072 c         2266 g         1850 t
ORIGIN      
        1 tttctttact gaaaagaaaa aaagaagcaa aaaaagaaaa gagttcgcat tccacctgta
       61 ctacaaggtc aagtatcacc cgaagattac acatcaacaa aactcccccg gcggggcata
      121 caggcggctg ccgccgccgt aacactaccc tttttacagt tgaaattggg gcgtaaggtc
      181 agcggcgact cgccgcttga ccgaaaaagg gcaccctctg tagcagagag tgccctttga
      241 aaatttgctg tgtatttcct gaaacttaca gagttacctt gtcctgcaaa tagcttacca
      301 gttcatcgat cggcatgcga acctgttcca tagtgtcgcg gtcgcggacg gtgacgcagt
      361 tgtccttttc tatggtgtcg aagtctaccg tgatgcagaa cggagtaccg atctcgtcct
      421 gacggcggta acgcttaccg atagagccgg tttcgtcgta gtctacagag aagtgctttg
      481 ccagaacggc gtatacttcc cgagccttgt cgcccagctt cttggacagc ggcagaaccg
      541 cagccttgta cggtgccagt gccggatgca ggtgcattac cacacgggtg tcgttctttt
      601 ctgcatctac cacttcttcg tcataagcct ctgtcacaat ggacaggaac agacgctcta
      661 cgcccagaga aggctcgata acgtaaggaa tatacttggt attttcttcc tgatccatgt
      721 attccagcga cttgccgctg tgttcctgat gctgtgtcag gtcgtagttg gttctgtctg
      781 caacacccca cagttcgccc cagccgaagc tgaacttgta ttcgaagtcg gtggtcgcct
      841 tggagtagaa gcacagttcc tccggatcgt ggtcacgcag ccgcaggttt tcttccttca
      901 gacccagtgt cagcaggaag ttcttgcagt agcttctcca gtactggaac cattccagat
      961 cggtgtccgg cttacagaag aactccagtt ccatctgctc gaattcgcgg acgcggaaga
     1021 tgaagttgcc cggagtgatc tcgtttcgga aagacttacc gatctgagcc acgccgaacg
     1081 gcagcttttt gcgggtggta cgctggatat tggcaaagtt tacaaagatg ccctgtgcag
     1141 tttccggacg caggtacagt tcggacttgg aattctcggt tacaccctga taggtcttga
     1201 acatcaggtt gaactgccga atgtcggtga agtccgtctt gccgcagttc gggcagggga
     1261 tctgcttttc gcggatatag tccatcatct gctgattggt ccagccggca gggttggtgc
     1321 cgtcgaaatc ttcaatcagg ttgtccgcac ggtgtctggt cttgcaggac ttgcagtcca
     1381 tcagcgggtc agagaaaccg cccagatgac cggaagccac ccatgtttcc ggattcataa
     1441 tgatcgaagc gtccagaccg acgttgtacg gggactcctg tacaaacttc tgccaccatg
     1501 cacgcttgat gttgtttttc agttccacac ccagagggcc gtagtcccag gagtttgcca
     1561 gaccgccgta gatctcagag cccgggtata caaagccgcg ggacttgcag aggtttacga
     1621 ttttatccat gcttttctga tcgttggtca acattttcgt attctccctt gtttgaattt
     1681 gcttgctgta tacagcattg gtatctttta tattgtaccg gaaaactgcg gaaatgtcaa
     1741 gtgttttgtg agaatgaaaa taatcgggaa ggattctcta aatccccagt gcctccctga
     1801 cagtcatcag gtcgttctga gtgtcctttt cgccgtcatc ccgccagatc atcagtgcat
     1861 aatctgcacc gctgtcgggc ataatgccgt atagggtgcg ttcttcgttg tcgggatagt
     1921 agtcgatcag ccaggattca tcctgcactg cgatctcgtg ctctctggta ctgtactggc
     1981 taagttcgtc cagccattcc tgcatattgt tataatatgt cgttgtactg atctccagga
     2041 agatggtgcg tccctgctcg gcgtcgtaga aggtatagtt atagtatgca ttatcaatgt
     2101 atcccagccg gaattcccag ctgtccagga ctgcctgcgg tggctggtat tttgtaatgc
     2161 caggctgttt ttcgggaaag tctgcggtaa gtgcgtccag cgtttcgtat tctgtgtagg
     2221 tgatagggtc tttgtaaacg acttcttcgg tggtttctgt tgtggcaact gtacttttat
     2281 ttttttgtgc tgtgttcgat gtcgtaccac aaccagcaca aaccaacatt gcaatagcaa
     2341 cggagaatat cagaattttt ttcatagaag ctacctcctc actacatttt taaaacggaa
     2401 tggtatgaaa ataacagacg taaaggtgta tatcttatag ctgcattata tcatatcgga
     2461 atataaaaat acaaaatacg tgttttcgca caaaataaat ttaattattt atgtaaaata
     2521 aataaaacac gtgcgtatat cgttgaatat acgcacgtgt ttcctatatt atttagtgct
     2581 tccgcaaata gaataccact ggcaatcgcc gcagatgtcc tgtagcagtc ccggttccag
     2641 aatgtgccgg cttacccggt cggtaaattc gctccaccgc aggacactgc ccacagaaag
     2701 accgcagaac atgagaacgg cactgtcata gcgttgtacc ttttccgccg catcgcacac
     2761 atgggacagg ctgtggggac agctggcaca gatgcagtcc gtgccgccgg tcagggtgat
     2821 atatgggtcg tcctgccgga acagccgtag catttccgtc atgtttgcgg taaatgcttc
     2881 gctgtagccg tgcccgcaga aaaacgccga gcacaagccg tggtgtggtc gtagtttata
     2941 cacgctggat cagccgcttc gggagccgct ttccaatcag gattgccaga gccattttta
     3001 cgcagtccgg cagaatgaac gggaatacgc accagcccag tgcagtcagc agtcccacag
     3061 aaccggaatt cttggcgtat acgatcatga accacgcagt accaaaggcg tagcacaccg
     3121 ccagccccag caccaatgcc agaatcatta ccggcagctt ctccccgaaa aagtgggtaa
     3181 tcagccagta gagcagaccg gtgaacagga aaccgataat gtagccgccg gtggtgccga
     3241 acagaatgcc gatgccgccg gtaaagccgg cgaataccgg aacgcccacg gctcccagta
     3301 caatatagat cagaatggaa atggtgccgc ccttgccgcc cagtgtggcg agagcacaga
     3361 acacggcaaa ggtctgcatg gtgaacggca cggtcatggg aatctggatc catgagcata
     3421 cggcaatgat cgccgcgaac agggcaatct gcaccattgc cacgatcttc tggtgctggg
     3481 tcttcttggc agtcatgccg gattcggcag ctgcggtctg tgtttgctgt ttcataggaa
     3541 aaagtccttt ctggattgga ttggagagcg tcggcttgcg ggaaattccc cgccgcaaag
     3601 cgtgtgctaa cgctgcaacg ttcccattat agcacagtcg gccttatttg tcaacctatt
     3661 tgaaagagaa atcgggtgac aaatcgcaca aactttgcaa acaggcttca aatttgcaca
     3721 aaatgcacat ttttataaat ctaattttgt gcattcctac aaaagttgaa aaagcccttg
     3781 acaatcgtcc ggaatcatgg taaatttata ttagcactca gagagaatga gtgctaacac
     3841 tttaaaagtg aatctgccag aaaggatggt tattgtggat gcacgaaaac gaagaattct
     3901 atcggcggta gtcgatctgt atattcggac cggtgagccc gtggggtcga aaacgatctc
     3961 gcagttaccg gacattcatg tgtctgcggc aactgtccgg aacgacatgg cggtattgga
     4021 agaactgggg tatctggaac agccgcacac ctctgcgggg cgtgtgccta cctttaatgg
     4081 gtaccgcatg tacatcgagg agatcagtgc cgagggaacg ctcccccaag aggaacggga
     4141 ccggctggac gagatgctgg gcgatgagag tcagctgaca gaagacctga tcctgcaaag
     4201 tgccaccacg gcactttccc agatcaccaa gtgtgccacc gtggtttcca acttcgcacc
     4261 gaaatattcg gtgatctcga aagttgacgt gatccctaca gggaaacgca tgtatgtggt
     4321 gctgctgatc acatcggacg gcaagatcaa gaatcgtgcc tgccggttac agtttgacct
     4381 cacccatgaa cacatggact ttttcaagca cttcatggag gaaaatctgg agggcgtttc
     4441 ggtttccgac ttgtcggaag aaaagctgga acagatgatt accgccatgg gcacttacat
     4501 gatgacgtta agtccgctgg tgcagggcat ttgcgaaatg tccaaggatt tgcagcagaa
     4561 tgagttgtat atgaccggtg agcagaacct gttctcctgt gacgatctgg acaaggcgga
     4621 agtcgccaag ttcatgatgc acaaacagga attatccgga ctgctggatg attcgtttca
     4681 gggcatcaag gtcatgttcg gtgaggaagg caatgatttc gtcatcggca actccagtat
     4741 gatcgtttcc aagtatcaga aaggcgatca caccgccggt tcgctgggtg tcatcggacc
     4801 gatgcggctg aattacgcca aagtgatacc ttatatagaa tattttaccg aaaaaatctc
     4861 acacctgatt tcggacgacg acgatacgcc gccgccggaa ctggaatgta tcgggcagac
     4921 ggagccgcca aaggatccca agccggaaca atagaaagga cgtgttagca atggcagacg
     4981 aaaaggaaac caagaagccg gaaaccgaag cacagcagcc ggaagatgca aagacagaga
     5041 acgcaccgga agaagctgct gcaccggaag ccaaagccgg ggaaaccaaa gctgcggacg
     5101 gcaaggacga caaaaagaag aagaaaaaag acttgaaaga tgcactggca cagaaagaag
     5161 cagaactggc ggaacaaaaa gaccagcacc tgcggctgat ggcagagtat cagaactacc
     5221 gcaaccgcac caccgaggag aaaaagaaga tctacggtga tgccaaggtg gactgtgtaa
     5281 aggaactgct gaatgtcatg gatacgttcg aacgggctct ggaagcacct tgtgccgatg
     5341 agaactataa aaagggcgtg aaactgacat tcacacagat tcagaaagca ttcgaaaagc
     5401 tgggcgtgac agaagttgcc gcactgggtg aaacctttga cccgaacctt cacaatgcga
     5461 tcaagcaggt ggacgacagc gactacgaaa gcgacaaggt ctgcgaggtg ttccagaaag
     5521 gatacctgct gggggatcgg ctgatccgtc cggcaatggt tgccgtttct gtgtaggcag
     5581 gaggcagcag cgtgaagaaa cacacagcgt ccctcatgac ggcggcgatg ctgctgacag
     5641 tggcgtggct gacagggtgt gattccaagg aactggaaca gcctgccggc accggtactg
     5701 caccggcact ggaaaccgtt gcggagccgg taacggaagc agcaaccgaa gcgatagaag
     5761 ccacggaggc agctgggcag agctttgacg attacgccgc gtttgctgcg gcaatggcag
     5821 agcagcatcc ggaaaagaca ctgtacacgc cgtcggaaag cgtgttggca gcgtggacgt
     5881 ggaaagcgat tcagctgaaa gcggacggtt atcagtacca gctgtacaac gccgaaacgc
     5941 aggaaacggt aaacctcctg atcagcatgg agccgctgtt tcagaatgca caggaaattg
     6001 tggattccat ggcagatctg acagatgtca cagcgacact ggtcgatgca ggttgctgcc
     6061 tgtgccggtg gaacgacagc ggcgaatatg ccctgtatgg catcaccggt gacatgggtg
     6121 ccaattatat tgtcacactg gagcatgcag atcagacgcc ggcttcggca gacgaactca
     6181 ccgcactccg cagtgagttc tggctgtaaa gcaaacttct atccgcaagg gtagtttaaa
     6241 tatatttatt caatgagaaa atctgcccgt ttttccgcag gggaaacgga gatacagaaa
     6301 ggaagaattt actatgggaa agattattgg tattgacttg ggtacaacaa actcctgtgt
     6361 tgccgttatg gaaggcggta atgcagtcgt aatccccaac gcagaaggtg cgagaactac
     6421 gccgtctgtt gtcggcttct ccaagaatgg tgagcgtctg gtaggtcaga ccgcaaagcg
     6481 tcaggcaatt gcaaatccgg agcgtaccat ttcctccatc aagcgtcaca tgggttctga
     6541 ctacaaggtt gacattgacg gcaagaagta caccccgcag gaaatttccg caatgatcct
     6601 ccagaagctg aagaccgatg cagaagcata tctgggtcag ccggtaaccg aagcagttat
     6661 cactgttccg gcatacttca ccgactctca gcgtcaggca accaaggacg ccggacagat
     6721 cgcaggtctg actgtaaagc gtatcatcaa cgagccgact gctgctgcac tttcttatgg
     6781 tatcgataag gaagacgacc agagagttat ggtatacgac ctcggcggcg gtacattcga
     6841 tgtttccatt atcgaaatgg gcgacggcgt acagcaggtt ctggctaccg caggtaacaa
     6901 ccgtctgggc ggcgacgact tcgaccagag aatcatcaac tggatggtag aagaattcaa
     6961 gaagactgag ggcattgacc tctccaacga taagatggca gtacagcgtc tgaaagatgc
     7021 cgcagaaaag gcaaagatcg agctgtcctc caccaccacc accaacatca acattccgtt
     7081 catcaccgct gatgcaaccg gtgcaaagca cctggatatg aacctgacag ttgcaaagtt
     7141 caacgaactg accaaggact tggtagacgc taccatggga ccggtacagc aggctctgtc
     7201 tgacagcggt ctgtccccgt ctgacctgaa caagatcctg atggtcggcg gttcttccag
     7261 aattccggca gtacaggaag ctgttcgcca gaagctgggc aaggaaccgt tcaagggcat
     7321 caatccggac gaatgcgttg ctctgggtgc tgcatatcag ggcggtgtcc tcggcggcga
     7381 tgtcaaggac ggtctgctgc tgctggacgt tacaccgttg tctttgggtc tggaaaccat
     7441 gggcggtgtc tgcaccaaga tcattgagag aaacaccacc attccgacca agaagagcca
     7501 gatcttctcc actgcggcag acaaccagtc cgcagtagat attgttgtcc tgcagggtga
     7561 acgtgagttc gcaaaggaca acaagcagct gggcaccttc cgtctggacg gcattgcacc
     7621 ggctccgaga ggggtaccgc agatcgaagt aacattcgac atcgaccaga acggtatcgt
     7681 acacgtgact gcaaaggatc tgggcaccgg caaggaacag gacatcaaga tcactgcttc
     7741 caccaacatg tccaaggagg acatcgacaa ggctgtcaag gaagcagagc agtatgcaga
     7801 agacgacaag aagcgtcgtg aagaagtaga taccaagaac aacgcagaga gcctgtgctt
     7861 ccagtgcgag aacgctctga aggaattcgg cgacaaggtt ccggcagacg aaaagtccgc
     7921 aatcgaaagc aagatcgcag acctgagaag tgcactgggc ggaaatgatg ccgcagcgat
     7981 caaggcaaag tccgacgacc tgcagcaggc attctacgca ctgtcctcca aggtatacaa
     8041 gcagaacggc ggtcagccgg gtgctgatcc gaacatgggc ggttccgcag gctttaacag
     8101 tgctgacccg aatatgggcg gcaacccgaa cggcggttcc ggtgatgacg gcgttgttga
     8161 cgcagacttc aaggaagttt aagaatgaaa cgcagcggca tgaaatatga cgtgccgctg
     8221 tgaatagaac ggcggtgacg gggcaggttt cggcttgtcc cgacatcgtc gtatatgggg
     8281 actccccgca cggtgtgccg ctgcaagcgg acacagcgta acataatagg gcaataccgc
     8341 acaaagagcg tctggcgact ttgcacgcca tctgcttgca
//
LOCUS       DAWBGR010000110         8359 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2308_length_8359_cov_5.432322, whole genome shotgun sequence.
ACCESSION   DAWBGR010000110 DAWBGR010000000
VERSION     DAWBGR010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8359)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2308_length_8359_cov_5.432322"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2050 a         2192 c         2419 g         1698 t
ORIGIN      
        1 cagggagccg tgaaagtcaa cggcgaagtc tgcaccatgc gtggcaagaa aattcgcccg
       61 ggggatacgg tatccgtcga taaatttacg gtacaggtga cggcagaagc atgaaactga
      121 ctgccctgtc cgtggacggc ttcaagaatc tcaagggggt ttccctgatt cccgatccgt
      181 cctataatct gattctgggc gaaaatgcac agggtaagac caacctgctg gaggcgatct
      241 ggacactcac cggctgccgc agctttcgcg gtacgcggga acgggactat ctcccctttt
      301 ccggcggcaa tttgcagatc gaagcgaaat tccaggatgc ccgacgggaa cagtctgtcc
      361 ggctgtgcat ggtttccggt accgtaccgg aaaaaaagat ctggtgcaac gatgttcccc
      421 agaaaagcag tgccctgttt tccgtgttcc actgcgtcac atttacgccg gaagacctct
      481 ctctcgtaga gggcggtccg gaacggcggc ggacgtttct ggacttgtgc agtgcccagc
      541 tgcacgcccc tatgctgggg ctggtgcggc ggtatcagct ggcgttgcag cagcgaaatg
      601 ctgtgctgaa acaacggctg ccggaggcac aggcggcagg acttctggag atctgggaca
      661 gacagctgga acagcttggc accgagatcg ccgtccagcg gggcatctac gtccggcggc
      721 tggcggaagt ctgcgtgccg ctgtacgccc agatcgcacg gggacgggaa accgtttccc
      781 ttcactatca gtccagcgtg ttcggaaaag acgcacagga caccctgccg gaaacgcgga
      841 caccggaact ggtgcagcag tacggcgaaa aaatgcggca gagccgcagc tccgacctgc
      901 tgctgggaca tacggcaagc ggggctcatc gggatgacct gctcctcgcc attgacggca
      961 agcctgcaaa ggaattcggc tcgcaggggc agaaaaaaag tctggctctg gctctgaagc
     1021 tggcacaggc ggagatctac agcaaacgcc ggaaagaatc accggtggtg ctgctggacg
     1081 atgtcatggg cgaactggac gaggctcgtc aggcggtggt ttccaccatc gtgcaggaga
     1141 tgcaggtctt tgtgaccacc tgtcaccccg aatccctgct ttccccaaag aacggcaagc
     1201 ggtttttgtt gaaagacggc atgctgaccg gcgatgtgga aaatcgtccc gaaactgctt
     1261 gaaattccgt tttccacaga ctgttgaaaa cgctgtggaa aacttgtttg aagctgtgaa
     1321 aaagagaaat acacggcgaa tttttgtgga ttccacagaa gtctgccgca aaaagaaatc
     1381 attcgaaatc caaactactc ccgcttcggc gggaaatcga gggaatcaaa tatgacagaa
     1441 gaaatgaaac aaggtgcaca gaattatacg gaggatcaga ttcaggtgct ggaaggtctg
     1501 gaggcggttc gaaagcgtcc gggcatgtac attggatcga ctggtcccaa aggactgcac
     1561 cacttggtct acgagatcgt agacaactcc attgatgagg cgttggcagg attctgtacc
     1621 gaaattactg tagatattct gccggaaaat gtcattcgcg tatccgataa cggacgcggt
     1681 attccggtag gcattcaccc gaaagagggc atctctgcgg caactgtcgt gtataccatt
     1741 ctccatgccg gcggtaagtt cggcggcggc ggatataaag tttccggcgg tctgcacggt
     1801 gtcggtgcgt ctgtcgtaaa cgccctgtcg gaatggctga agctgaccgt caaggacggc
     1861 aaacacgtgt attttcagga gttccaccgc ggcagctatg acgaactgct ccgtgtgatc
     1921 ggggacacca ccgaaaccgg taccaccgtc gagtttaagg cagatgcaga aattttcgag
     1981 gaaactactg agtacgacta tgaaattctg ctgaagcgtc tgcgggaaca ggcgttcctg
     2041 aatgccggta tccgcattgt cttttccgat aagcggaacg aagcagaacc ccgcacggaa
     2101 gtgctccact atgagggcgg tatccgccag ttcgtggaat atctccacca gaagcgtggt
     2161 ctggaagccc tgtcggaaga cgtgatctac gtttccggca tggacggcga ttctttcgca
     2221 gaagtggctc tccagtacaa cgacagctac aacgagatga tcctctcgtt tgccaataac
     2281 attcacaccg ccgacggcgg tactcacgag gtgggcttca agaatgccct gaccaaagtc
     2341 atcaacgaat acggcaaaaa gttcggtctg ctcaaggacg gacagaagct tctgggcgat
     2401 gacgtgcggg aaggtctgac ggcgatcgtt tccgttaagc tgaccgagtg cgaattcgag
     2461 ggacagacca agggccgtct gggcaatccg gcagtcaagc ccttcgtgga gaaaatggtc
     2521 tttgataagc tgatgagcta cttcgaggaa aatcccaccg tggcacgggc aattttcgac
     2581 aagagcgtgt ccgcacaggt ggcacgggaa gcggctcgga aggcaaagga aacggcacgc
     2641 cgcaagtccg ccatggagag tgcctctctc cccggcaagc tggcagactg tcaggaaaag
     2701 tccaccgagg gcacggaact gtatatcgtc gagggtgact ctgcgggcgg ttccgccaag
     2761 gagggacgtg accgccgcta tcaggcgatc ctgccgctct ggggcaagat gctgaacgtg
     2821 gaaaaggctc gtatcgaccg cgtttacggc aacgaaaagc tgatgccggt cgttactgca
     2881 cttggcacct ccatcggcga ggatttcgac attcagaagc tgcggtacgg caaagtcatc
     2941 atcatggcag atgccgatgt ggacggttcg catatccgca ccctgctgct gacgttcttc
     3001 ttccggttca tgcggccgct gattaccaac ggcaacatct atctggcaca gccgccgctg
     3061 ttcaaggtgt ttaagggcaa gaagtacaag tacgcattcg atgaagccga acgggacaag
     3121 tacattgcgg aattcgccga tgtcgacggc agcactgcca aggtcggcgt acagcggtac
     3181 aaaggtctgg gtgaaatgag ttccgaacag ctgtgggaaa ccaccatgaa tccggaaacc
     3241 cgttctatga tccgcgtgga catggaggac gcagaaaagg cggacgaaat tttcacgatc
     3301 ctcatgggcg ataaggttgc tccccgtaag gagtttatcg agaagaatgc acgctatgtg
     3361 cagaatctgg acgtataaag aggaaatacc atggaaaaca caacaacgct gccggtattc
     3421 gacaccactg ccccgtatca catcaagcgg gagtattatc gggacggttt cacggcattt
     3481 cagaaaaagt ttgtcatgaa acgcaactat gtgatgatgg cactgttttt cgttctgctg
     3541 gtgtcgttcg tggtgtcggc agtggcagac cccagcaata agacggcata ctttctcatg
     3601 atgatctgcc ttgcggcgat ctttatgctg tggtacaacc cgcggaaaca gcgtcgcatg
     3661 gtgctggacg cggtgcagga actggaattc gaacagttca ccgcctgcaa tgacggcaag
     3721 gtgctgcgga tccagatcca acagggcgag gacgaaaccg agcagatccc cgaatcccga
     3781 atccccctgg cgactgccta tgtgcaggag ttcgaggaat tctatctggt ctgcgacggc
     3841 aagaagatgt tctacgtgct gccgaaatca gcactggaac agtccgccga agcactgccg
     3901 gagcagtcag cagaggaagc gtgactgttt cacgtgaaac atggactgct tccggtgaca
     3961 tgtcagcaga tacagcacag cttgtccctg aaccattact gcaaagcaat ccctcagtca
     4021 gtgcttcgca ctgccagctc ccttttcaag ggagccaaat catgcagcat tgtcttatgt
     4081 ttcacgtgaa acacaaaccc atacgacaaa acaccccgaa agggaaaagg cggttttgcc
     4141 gccgtgatag gaatatagaa tgatggataa taacacttca atggaaaaag aaaatttctt
     4201 taccaacgga aaacaaaaaa ttacccccat cgaaattggt cgggagatgc aggactcgtt
     4261 cctgcaatac gccatgtcgg tcatcgttgc ccgtgccctg ccggacgtgc gggacggtct
     4321 gaaaccggta caccggcgta tcctctacac catgttcgaa aagggactga caccggataa
     4381 ggcgtaccac aagtgtgcgg ataccgtcgg tgccgtgctg ggtagctatc acccccacgg
     4441 tgacgcgtcg gtttatgatg cactggtacg tctggcacag agtttctcaa tgcggtatat
     4501 gctggtagac ggtcagggta acttcggttc cgtagacggc gacccgcctg ctgcttaccg
     4561 ttataccgaa gccagaatgt ccaagattgc cttggaaatg ctgacagaca ttcacaaaga
     4621 aacggtgccc tttgtcccca actatgacga ccgcctgaaa gaaccggaag tgcttccctc
     4681 ccgtttcccg aacattctgg taaacggctc tgtgggtatc gcagtcggca tggcgaccaa
     4741 tatcccgccc cataatctgg gcgaggtcat tgacggcatt cacatgctga tggaaaatcc
     4801 ggactgcacc atggacgaac tgatgacctg tgtgcaggga ccggacttcc ccaccggcgg
     4861 cattctcatg ggcaaaagcg gtatccgtgc ggcgtatgcc accggtaagg gcaagctgac
     4921 gctccgcgga cagactcatt tcgaaaaggt tcgcggacgg caggcgatca tcgtgacgga
     4981 aattccgtac atggtgaaca aggctcgtct ggtgaagtcc attgccgatc tggcaaagga
     5041 taagcggatc gaaggcattt cgcacattca ggacgaatcc agccgtgagg gcatgcggat
     5101 cgttatcgaa ctgaaaaaag atgccaacga gcagatcgtg ctgaacaagc tgttcaccta
     5161 tacccagttg caggataccg tcagcgtcaa tatgctggcg ttggtgggcg gtgagcccaa
     5221 gactctgagc ctcaaacagg tgctggaaga atacctgaaa tttcaggtgg aagtcatcac
     5281 caaccgttcc aagttcgatc tggaaaaggc aaagaagcgt gcccatatcc ttcagggttt
     5341 cctgctggcg atcgactata tcgacgaagt cattgccatt ctgcgggaca gcaagaccgt
     5401 tccggaaggt aaggctcgcc tgatcgagcg tttcaaggac gtggatatgt cggctctcct
     5461 cgaccgtgca caatacgaca tggagggcta ccagctggaa cacaagcagg ggctgaccga
     5521 ggagcaggca gaggcaatcg tacagatgcg tctgggacag ctgaccggca tggaacggca
     5581 gaaagtcacc gacgaactgt atgccctgat ccagaagatc accgacctgc tggacattct
     5641 ggcaaatcgg gaacgggtat accagatcat catggacgac ctcaacgaga ttcgccggaa
     5701 gttcggcgat gcccgccgca cacagatcga aacagtcgag ggcgaagtgg acatcgagga
     5761 tctgattccg gtggaggact gcgtagtcac ctataccgag aacggctata tcaagcgaat
     5821 ccctgccgat gtgtataaga gccagcggcg tggcggccgc ggtgtcaccg gcatgaaaca
     5881 gcgggaggag gacttcgtca gcgaactgat gattagttct tcccactccc atatcctgtt
     5941 tatcagcaat ctgggaacta tgtataagct gaagtgctac gaactgccgg agggttccaa
     6001 gaatgcccgc ggcaccaata tcgtgaacct gctgcccctg aacgccggag aaaaaattgc
     6061 cgcagtactc accacgccgg acttcgacga gggcaagtat gtggtcatga ttacccgcaa
     6121 tggtaagatc aagcggacag cactgtccga ctataagaac gtgcggaaga acggtctgat
     6181 tgccattggt ctggacgagg gcgacgagat cgccgcagtg cgtctgacca acggctccaa
     6241 ccggctgttt gttgccaccc gcaacggtat ggcaattcgc attgacgaaa gcgatgttcg
     6301 ccccatgtcc cgttcggcac acggtgtcaa ggcaatcacc ctgcgaaagg gcgatgccgt
     6361 ggtatctatg gcaagagagc gtgagggtgc ttccctgctg actgtgacgg aaaacggctt
     6421 cggcaaaaag accgccctgt cagaataccg catgcagtcc cgcggcggtc tgggacttac
     6481 caactacaag gtggacgagg aacacggtgc agtctgcggc attaaggtcg tggacgaaag
     6541 cgacgacctg atgttcatct ccagtgacgg cgttatcatt cgtgtggcat gcagcgagat
     6601 tcgtgccatg ggacgtgttg ccaagggtgt gcgtgtcatg cgtgtcaacg agggacaaaa
     6661 ggtagttgcc tttacccgta ccgaagcggc acaggacgag gacgatgcag agagccttca
     6721 ggacggctct gaacagcctg ttgtaacaga cgaggctatt cctgcacgag aggcagaaac
     6781 cccgtcagag ggcatcacag acagttctac agacgaatct gcggaatgat cggtgatata
     6841 cgacaaccga cagacaacca acaataaaga aaccagcagc ggcggaattt ctgccgctgt
     6901 tgttgtatgc ggcagcaggt cagtccgccg catgtttcac gtgaaacaaa ccacttaaaa
     6961 gaggtgtgtc atgatattct ggattttttt cctgctgctg attctgctgg gcatctggtt
     7021 ctgcattgag cagcatgttc tgcgggtgcg gaaacaaacg ctctcgctgg aacatctccc
     7081 tgccggctgg gacggcgttt ccctgctgca aatcaccgac ctgcaccacc ggcaaatggg
     7141 caggggcaac tgccgcatca ttcggcaggc acagcaattg cagccggacg tgatcgtcct
     7201 caccggtgac ataatctcgc gggatatgcg ggactttcac gaaatctcgt catttttgca
     7261 ggcactttcg aaaataagcc ccacctatct ctgcacggga aatcacgaac tggacttgcc
     7321 gccggaggtg cagcaagagt tctgggcaat ggcggagcag gcaggctgtc acccgctgcg
     7381 aaatgaaacg gttccgctgg aaaagtccgg ctgttccccg ctgtatctgg cgggagccga
     7441 actggcgtat gggatctatc gggacgacaa ccgcggattc cggcacttgc agccctatac
     7501 ggcggacgac ctgacacagg ctctgggcac acggcagggc tgcacggtgc tgctggcaca
     7561 taatcccctg ctgctggaca gctacgccgg ctggggtgca gatgtggtgt tgtccggaca
     7621 cgtccacggc ggcatggtgc ggctgccctt tgtaggcggc gtgctgtcgc cggagcgaag
     7681 atttttcccg aaatatgaca aggggctgta cgagaaaggc ggcacaaagc tgtatgtatc
     7741 cggcggcatc ggcaagccgc ggctgttcaa cccgccggag atcaacatga tctatctgaa
     7801 cagcgtccag aaataataga tcagcccgta taccatggaa gcggaagtgc cgtagagaat
     7861 gaccggcccc gcaatggcaa aaatgcgggt gccaatgccg aggacccagc cctctgtgcg
     7921 tgcctcaatg gcagaggaaa ccacaccgtt tgcaaagccg gtaatgggca ccagcgtgcc
     7981 ggctccggcg tgtttgccca gcttggcgta ccagcccaga ccagtgcaga ctgcactgac
     8041 tgccaccagc ccgatagaga tccacgcggc agctgttttc tgctccacgc cgcagaattc
     8101 cgtcagaata tggataccgg cttcgccgat cgtacagatc agaccgcctg tcagaaatgc
     8161 aagaggaatg tttttccacg acttggattt cggggcggtc tgattttgca tggtttgata
     8221 gagttcacga ctgattttca tgagaagccc tcctttttcc ggagtatgtg cagttttggt
     8281 gcagttatgc gtgaaatttt tgcttggggc aataccgcac aaagattgtg cgacagatgc
     8341 gtcgtcaaat ttttcgtca
//
LOCUS       DAWBGR010000111         8317 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2321_length_8317_cov_4.589809, whole genome shotgun sequence.
ACCESSION   DAWBGR010000111 DAWBGR010000000
VERSION     DAWBGR010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2321_length_8317_cov_4.589809"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2062 a         2108 c         2240 g         1907 t
ORIGIN      
        1 ccgccgtaac attacccttt ttactcttga aattggggcg tagggtcggc ggcgacccgc
       61 cgccgcgaaa ggatatgata attatgatta ctgtttctaa cgttagtctg caattcggcg
      121 gcgatacgct gttcaaaaat gtagacctgc aattcaaccc cggcaactgc tacggtgtta
      181 tcggtgcaaa cggtgccgga aaatccacat ttctgaaaat tctctgcggt cagctgaaac
      241 ctactaccgg tgaagtgacc attccgaaaa atctccgcat gagtgtcctc aaacaggatc
      301 acttcgccta tgatgcctat accgttctga ataccattat tatgggcaac cagcggctgt
      361 acgacatcat gcagcagaaa gatgccctct atgccaagga ggattttacc gaggaggacg
      421 gtattctggc ttctgaactg gaagcggaat tcgctgaact ggacggctgg gaggcggaat
      481 ccaacgcttc caagctgatt caggggctgg gtctgccgga aaaactgctc tatcagcaaa
      541 tggcagacct ctccggtaat gaaaaggtga aagtcctgct ggcacaggct ctgttcggca
      601 atccggatat tatcctcatg gacgagccga ccaaccatct ggatattgat gccattgcgt
      661 ggctggagga ctttttgtcc gagtacttcg gtaccgttat cgtggtttcc catgaccgtc
      721 acttcctcaa tgacgtttgc acccatatcg tggatattga ctataccaaa atcaaaatgt
      781 atgtgggcaa ctacgaattc tggtacgaat ccagccagat gatgcagcgg ctcatcaaac
      841 agcagaacaa aaaggttgaa gataaaatcg ccgaactgaa aacctttatt gcccgcttct
      901 ccgccaataa gtccaagtcc aaacaggcga cttcccgccg gaagctgctg gacaacctga
      961 ctgtggaaga actccctgcg tccagccgcc gctatccttt tatcggcttc gatatggatc
     1021 gggaactggg caaggaagtg ttgcaggtca gcggtatctc caagactgtg gacggcgtga
     1081 agctgttgaa caacgtcagc tttacgctgg gacgcaccga caaggtggcg tttatcggcg
     1141 agagcgaaca ggcgatcacc atgctgttca agattctcat ggaggaagaa cagccggacg
     1201 agggtacgtt caaatggggc gtgtctactt ccacttccta ttttccggtg gataactccg
     1261 catacttcaa cgacaacgat gattccattc tggactggat ccgccagtat tccacatcgg
     1321 acgaaaccga aacgttcctt cggggcttcc tgggcagaat gctgttctcc ggcgatgata
     1381 tttataagcc ggtcaaggtg ctgtccggcg gcgaaaaggt tcgctgcatg tttgcccgca
     1441 tgatgctgtt ccactccaat gtactgcttc tggacagacc taccaaccac ctcgatctgg
     1501 agagcatcac cgctgtcaat aacggtctgg tgaacttcaa gggcgtagtc attctgtctt
     1561 cccacgacca cgagattctg gaaactgtgg caaaccgcat tatcgagatc acgccggaag
     1621 gctgcatcga ccggcagggc acttatgagg aatatctgga gtggaaaaag gaaaacggga
     1681 aataaggcaa caccgcacaa agcccgcctg acggctttgc acgcaatctg ccgaaaaatc
     1741 tgataacgca tctgtcgcac aagctttgtg cggtgttgcc caaaattttc ctgcataact
     1801 ccgcccgctt cgggcatact gaaagtgtac cgttcgccgg aacctgcggc ggtaacttct
     1861 ttcagaactg gagcgatcat tatggagatc aaaaagaaaa acagcaacat tcactgttcc
     1921 gtgacccagt gcaagcacaa catggtcagc gaaaactact gcggtctgga ctgcatctgt
     1981 gtgggcactc acgaatccaa ccccactgta ccggaatgca ccaactgtga ttcctttgtc
     2041 aaacgctgcg gcacagagtg ctgaacacgc gtaccggagg atttttcctc cggctacata
     2101 gggaatcctt tgggaaatcg gctgttgatt cgtcatgttg cacaaaaaac aaggtgcatt
     2161 caaatgcaaa acggcaaaaa aagtgggaat cccttgtaca tttacgaaaa ttcgggtata
     2221 ataaaagagt atccaagttc cgaaggaggt agaattttca tgtatgataa aacaatgacg
     2281 ttttccgtca atgacgaaaa agagatcgaa atgaagaaaa cgctgatgct tgtatatgat
     2341 gcactcacga aaaagggata taatccggtg aatcagattg taggctacat tctctccgag
     2401 gatcccacct acataaccac atacaacaat gcaagaagcc tgatccgtca tatcgatcgg
     2461 gacgaactgt tacaggctct ggtaaagtcc tatctgcacc agtgagctgt cctttaaaca
     2521 attcgaaaaa accgcaccgc aggtctgcgt acaagcagaa agcggtgcgg tttttttgta
     2581 cgttgcactg gaaaatgtca atattgtatc ccacaaatcc gcataagaaa tacacgaatc
     2641 agcgaggctc ttttatccat tccgtcaatt gacagatcat gcaggatatg gtaaactaac
     2701 aataaaggag gggatccacc aaaaagcacc aaatacacaa aaaacgaatt cgtcagttgt
     2761 acattcatac aaataagcca gaaattcgtt gacaaaaatg tataatagtg ctataatatt
     2821 aaatagaagg ttctgtgcgt tttccgattt agcaatgaga ggaaggatac acgatgaaac
     2881 gcatttggaa aaaaatgatg gcgattttaa tggcagtatt tctggtatgc acaacattgg
     2941 tgcttgcggg cggagccgaa gctgtttctg cactggcaga ggcaggcggg aatacaccgg
     3001 aggtaacgga cggggcagat gcacagcagt tcgccgacaa ttccggcgat attattatag
     3061 ggaacatttc gatcgagctg gatcagctga tcaaaaacca ctatatggtc actgtgccgg
     3121 tcaccatgcc caataacccc ggctttaccg ttctgcaatt tggtgtaaaa tgggatgtga
     3181 cacgcatgtc cgtgcagggt gcacgttcta ccggcaacat gaagctgccg attctcacca
     3241 ttgccaatga caaaaggcag atctggatga tgttcattga gaacgactgc aaggaaacca
     3301 atatcagttc cctgacagca gagatcaatc cggatgtcaa ggtgggggat acatttgtgc
     3361 tggaaggggc ttatgcggac tatgccgaca acaaagctct gtacaaagac aagacgatga
     3421 aatcacatga cctgaatatt gtttccggta cgatcaccat tgtggacagc gttgtgccgg
     3481 aagtaaaact gcagatcgga gaagtcagac cttctatgcg ggatctggat gagaacgact
     3541 atatggtaga agtgccggtg accgcagaag cgaacaatgg ctttagcgac atggtgtttg
     3601 gcttccgctg ggatgccagc aagatgacgg cagaaccgcc gtccggcaat ataccggaag
     3661 ggctttctct gatccccacc attgacaacc aaagcggtgt cgggtggatc catgtcattg
     3721 ccgacagcac ctataccggc agcgacatct gtacgctgcg gttcacagta ccggacaata
     3781 cgaagccggg gtcgatctat gacattaccg gttgtagcag tgatggagat acagaggcat
     3841 cggttgccaa ccactccggc actgcgggta cgctgaccat caacaacggc agaatttttg
     3901 ccacttccac ccaggcggcg aattcttttg cattcggtaa ggtgacgatt ccggacattt
     3961 ccatttccat ggaagatctg gagcagagcg atcacctgat ctccgtgccg attacgatca
     4021 cgtcaaacag ctgctttacc atgctggcgt tcggtgtcag ctgggacacc aacgatttga
     4081 gcattgtcag ctgcacctgt gatgatacca agaatctggg catgatcgaa tcctattccg
     4141 atgacaacaa cggcatctgg atgcagtttc tctatcgcgg cccctatgat gcttttacag
     4201 gaacggcact gtgtacgctg acgttccgtg tgcggtcgga tatatcgctg ggcgacaaga
     4261 tcatgctgaa tccgcaggaa ctgagccccg acggcgacag catgattatc gtcagcacca
     4321 agggcacctt tggtgacctg aacatgcaaa agggcactat cagtctggtg tcggcaaacg
     4381 agctgactgc ggcggcaacc gtaaagatca gcgatgtgga aattgatacc tatcatttgt
     4441 ggctcaagag ctatgtggta gatgtgccca ttgtcctgaa acggaatacc ggtgtgtcct
     4501 atctgtctat gggcgttttc tatgatgaaa ccgttgcaca ggcacaagaa ctgcaaagcg
     4561 tggacattga ccagatcggc gtgctggacg actttagcca gagagccagt tcgggggcaa
     4621 gttccggctg gctggaattc cgcagtgtgg atcccggcag cgggtatgtc tacagcggca
     4681 ccacgctggg cgtgctgaag atcaaactgg acgaaagcgt caaggcaggc gatgtaatcg
     4741 acctgtctgc ggtatcgctc tcgcctaccg gtgcagtagc cacggtagag atcgccgacg
     4801 gttcccgttc ttctccggca ctggtttccg gctcgatccg catcacggaa gccgcaacca
     4861 caacaccggc ggaaacgacc acttcggcgg aacagactac cgaaacggaa accactgtga
     4921 caacgaccgc cacaacggaa gaaacgacga tttcggaaac agaaaccacc actgttacgg
     4981 aaacgacttc tgccggaact acaacagaaa cgactacgga aaccacagaa actaccaccg
     5041 aagaaaccac cacggaaacg gtgacagaaa ccactgtgac ttctacagaa actacagcag
     5101 cgactaccac aggatatacc gctgcaatca agactgacca cctgaaccgg acaacactgc
     5161 gggtacgtgc cggacaaacc agccgcctgc aattttatcc ggcagagggc aacggggacg
     5221 actgtgtctg gatgagttcg gatacagata ttgtcaagct tgctcctgga aacacctccg
     5281 cggaaatcat gctcacctgc aaccgtgtgg gaacggcaga ggtgtctgtg ctgtatgacg
     5341 ggcaggtgca tacctgtagc gtcagtgtgg tatccggcga cagcgaagct gccggcgatg
     5401 tcaacgtcga caatcagaca gatctgtctg atctggtgat gctgaatcac atgctgctgg
     5461 ggcagattcc cacaggagat gccgcatctc aaagtgctga catcaacggc gacgggtatc
     5521 tggacaactg tgacgttctg ctgctgatgc agatgctcct gaatcaggga ttgctacagt
     5581 aggctttttg aaatttagaa caagaaaagc ggtacagaat ttctgtgccg ctttttggtt
     5641 ctgtaaagac aacaccgcac aaagagcgtc tggcgacttt gtgcggtgtt gcctatgcaa
     5701 tttatgggtg agaaaaatgg gactgggcgt gcccattttt gcggctgcat tgacttctgg
     5761 taaaaaatat gctaaactga acaggagaac atttgtggcg gcactgtccg gaaaaggagg
     5821 gacttctgtg gtacaattct gggttggcat gcggcggctg acagcacact ggaaaccgct
     5881 gatactgttt gaaattcttt ggaaactggt gacgctgctg gtgattgcac cggcatgtgc
     5941 cggtttgatc cagctggcga tccatctggc gaagctgaaa tatctcacca catccaacct
     6001 gttacagttt ctccgttccc cgtggacgat tctgctgctg gcagtgctgt tgctgctggc
     6061 ggcgctgtat accctgtttg aaattgcggc agtctgtacc tgtttccggc agccccgctt
     6121 tcagaaagtc cgtaccacgc tggggcgaat ggtgcgttcc ggtttgcagt cggtgctgca
     6181 cttctttcgg ggcggcggac cgtttctggt gctgcatctg ctggtgctga ttccgctgat
     6241 gcagttttcg gcgacttccg gtatttttac cgcaatgggc attccggatt ttctcgccta
     6301 ctatatgacg aaaaaggaat tcctgctgcc gatttatgtg gcagcgatca ttctgtgctg
     6361 tctgctttct gttcgctggg tcttttccag cgtgctgttc actcagaacc agtgcagcta
     6421 ccgcagtgcc agagccacca gcgtacagct ggtgcggggg cgtttctggc agacgttttt
     6481 ctccgtgctg gtgtggaact gctgctattt cgccgctttg ctggtgtttc tgtgtctgat
     6541 tacggtggtc gtgctgatgg tgattcgtgc taccggcagc aatgatctca tcatgtcgca
     6601 ggcaatgcgg atcctgaaac tgctgattca gatcgtgctg tggagtttct cgttctttgc
     6661 cacgccgatc tgcatggcac acctgacggc tctgctggag aaacgctgtg tgcagatgcc
     6721 ggaggtggtg cttccggaac cggtcccgtt gagccggagt gccaagccgt ttcgccggag
     6781 cacggcagtt ctgacggcat gctgctttac ggtggcggct ctggggctga acctttcgta
     6841 tgtgtacagc gttttcaccg gcaaggcgaa tttccgtctg gcactgttcc agaatcccac
     6901 tgttatggca catcggggac tttctgccga tgcaccggag aacaccctct acgccttttc
     6961 cgatgccatt tcggtggggg cggatttcat tgaactggac gtgcagcaga ccagggacgg
     7021 cgtgctggtg gtgatgcacg attccaatct gaaacgcact accggtgtga acaaggacat
     7081 ctgggacgtg gactatgcgg acattcagaa tctggacgca ggcagctggt tcgatccggc
     7141 gtatgccaac gccagaatcc ccacgctgga agaaacgctg caattcgtgg acaagcgggc
     7201 gaagctgaac attgagatca agcccaccaa gcacggttcc gatacgctgg agcaggacgt
     7261 ggcggagtta attacccggt atcagtacac tgatgcctgc tatgtgacat cgttttccta
     7321 cggctcgctg aaaaaggtca aggaagtcaa tccggagatt cgcaccggct atctcatgag
     7381 tgtggcatac gggcagttct actccctgaa gtatgcggac gcgttcagtc tgaacaaggt
     7441 gttcgtcacc agtcaggtag tcaacgccgc ccaccagcag ggcaagcaga tattcgcatg
     7501 gacggtaaac agcatgtccg aggtgcggag cctgtgcaat ctccacgtgg acagcatcat
     7561 cacggacgat ccggtcatgg tgcagaatgt gatctcgcgg gacagcaccg gcgaaacgct
     7621 gcggtctgtg ctggactatt ttatcaattg aggggaattt tgcagtcggg ctggcagccc
     7681 atgcatctat ttgtcaacgg tttacacccc acctcacgaa aggagaatcc ccatgcacct
     7741 attcgccgcc cccgttcccg ccggcaaaaa ggctctggaa cacgaaaccg cttaccggct
     7801 gctggcgtat gcggtgcagg aggtttacgg gctgcccctg ccggaaattg cctgcgactc
     7861 ccgcggcaag cccttttttc cacagcggca ggagatctgt ttcaacctta gccactgccg
     7921 cgggctggca gtctgtggca tctcggcgtt tccgctgggg gtagactgcg aaagcattcg
     7981 ctctctgcgg gagggggtgc tgcggcgttc ctttgcaccg gaggaggtgc ttgctgtcat
     8041 ggaaagcccc cagccggacg aaactttttt ccggttctgg acgctgaaag aagcgtttgt
     8101 caaggctctg ggcgtgggca tctcctaccc gctgcacacg gcggtgtttg cgttggacgg
     8161 ggaagatgtc actcgccacc cccagggctg gcagttccgg cagttccggc cggacagcgg
     8221 gcatgtggtg tcctgctgcg gagcatcgga ggacacgctg ccggaagaac tccacgttgt
     8281 gtcattctaa ggaaacactg aataaagccc gcctgac
//
LOCUS       DAWBGR010000112         8313 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2324_length_8313_cov_3.444660, whole genome shotgun sequence.
ACCESSION   DAWBGR010000112 DAWBGR010000000
VERSION     DAWBGR010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2324_length_8313_cov_3.444660"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6247..6256
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2081 a         2008 c         2341 g         1873 t
ORIGIN      
        1 tgcaagcaga ttgcgtgcaa agccgtcagg cgggctttat gcagtgtttc cttagggcaa
       61 cactgaatca cagagaaagg aggcagtcca tgccgatttg tgaaccgaaa cagctttccg
      121 cccagattcg aaagggcgaa ctgcaaaatc tttactatat ttacggtgcc gatctggtgc
      181 aggtgaaaca gctgactgcc agcctgctga aaaaagccac cggcggtaat ccggacatgg
      241 cactgacccg atttgacggg cagaagctgg aactcaaggc attggaagat gctgcccgcc
      301 agttttccat gttttccgcc tataactgca ttcaggtgca taaccttgat gccaacaccc
      361 tgcgggaaga tcagctgaaa caactgctgg aactgctttc tgatgtgggc ggtgccacag
      421 tgctggtgtt tgatgtcacc ggatttgacc cgaaaaacgg caaaaaaacg ccgcagggga
      481 aaaacaaaaa gctgatagac ggcatcgcca aacacggtgt tgtctgtgag gcagtgcagc
      541 gtaccccgca ggtgctggca aaggaactgt cagaacttgc agaaaagcgt ggctgtatat
      601 ttcccagaga gagtgcggaa gaactggtgc ggctctgtat gggcaatacg ctgattctcc
      661 gcagcgaact ggaaaaactc tgtgcctatg ccgataaggg cggcacgatc accacggcaa
      721 tggtgcggga gatgacctca cctcagttgg aatccaccac gtttcagctg gcaaaagccg
      781 tggtttccca gaaaccgaaa gcggcaatgg cggaactgga taagctgtac gccatgcgaa
      841 tcaaccgcac attcattgtg cacgcgatcg catcttcctt tctggatttg tatcgtgcag
      901 ctgcggcact ccgagccgga aaacaggcgg cagacatgaa agccgatttc ggatatgctt
      961 ttgattttat ggtgcagaac gcgtttcggg actgccgccg tctgccgccg gaacggcttc
     1021 gtgcctgcat tcgggtgctg cgggaactgg aactggactt gaattccact tccggagaag
     1081 aacgcgtcat gctggaagcg gcaattgtga aaatgcttgc cattgcagga ggtacatatg
     1141 attcatatcg cacaggcaat tatcgttgag ggaaagtacg acaagatcaa gctttcctca
     1201 atcttagatg ctgtgattct ggtgacaaac gggtttcaca tttttcacga tgcagagaaa
     1261 atgtccctga tacggtacta tgccaagact acaggggtga ttatcctgac ggattccgac
     1321 accgccggtt tcaagatccg tcactacctc aaaggggcag tgcatgaggg aaagctgtac
     1381 catgtgtaca ttccggacat ttacggaaaa gaacggcgga aagataaacc gtcagccgag
     1441 ggaaagctgg gcgtagaggg catcgacaaa aagcggctgc tggaagcgtt cgggaaagca
     1501 ggcattctgg cagaggaagc accggaaaag acagacccga tcaccaagct ggatttgtac
     1561 gaactgggac tttccggcgg agcagacagc aagaaccgcc gcaaagccct gcaaaaaaag
     1621 ctgggactgc cggaactgct ttctgcggca tctctgctgg aagtgctgaa taccatgatg
     1681 acacgggaag aactgctgaa aatgctggca taagcagggg agaggtctat gcaatttgca
     1741 tcatttacat tgatatttct gtttttgccc attaccatgc tgctctttta cctgacacca
     1801 aaccgctgga aacgggaagt catgctgggc atcagtctgt tgtttttgtg cagcggcggc
     1861 tggctggcgg cggcggtgga aattttgctg acagccctga cctatggagc aggcttgctg
     1921 ctggaacatc tgcggcagcg aaaacacttt tccagactgg ttctgaccgg cagtatcctg
     1981 ctgcacttcg gggcactgct gctgttgcga agtgactggc tgttttcact caaggcaggg
     2041 tggttttccg gaaagtcgct gttcccgctg ggactggcat ttttcaccct gcaaagcgta
     2101 gggtattgca ttgacgtgtt ccgcggtaag atacacggtg aaaaaaactg gcggcagctg
     2161 ggactgtatc tgctgtttta tcctcgcctg atcatgggac cggtcgtgtc ctacagcgtt
     2221 gcggtgaagt ccctgtgtac gccggtgttt gacattgcac gtgtgggaca gggcttgctg
     2281 cggtttctgg tagggctggg aaaaaagctg attctggcga actggctggg catgctgttt
     2341 cagacgctct gtcaggcaga caccggcacc tattctctga tgctggtctg gctgggagca
     2401 tttgcccagc tgatgacctt gtatctggaa ctgtccggct atgcagatat ggcgatcggc
     2461 ttggggctct gctatggtgt ccgactgccg gaaagcttcg gaaagtcgtt gttctatccg
     2521 tccatggcgg catttgccga ccagtggaac cgcaccgtag tgcagtggtt ctcccactat
     2581 gtgggaacgc acttccgcgg gaaaaaccat tttctctacc ttttcgccat gctggcaaca
     2641 tggggctgta tcggtctttg gtatggcttc cggctgccgt cgctgctgtt cggactgctg
     2701 atcggcggag cactctggct ggagcatctt ctctggaaaa actcgtatca ctctgtaatt
     2761 cgctatatcc tcacggcggt gctgctctcg ctgggagctg tgctgctgtc gctgccggac
     2821 ttgcagtccg tctggtcgta tgtccgcatc atggtaggga ctggcagtct ggcacccact
     2881 gaagctgatg cagacgtgct gcgtgcgtat ggactgattt ttctgaccgc agtctacagc
     2941 gtgtccggaa attggcggta cctgctgcgg cgggcagagg aacagtcctg gttccgtctg
     3001 ctgcggacac cgctgacgct gcttgctgcg ttgctgcttc tgtcagcaaa caccgccttt
     3061 ctgatacaga ccggcggtgc agtgtccatg cagctgttgc tctgagggag gtgcatgctc
     3121 ttgaaaatca aacaagtccg ccgatggaaa aaacagactg tggcggagca ggcaggcatt
     3181 gcacggcgat tttccggcag actgctctgg gcagttttgc tgctgggcgg tatcggcggg
     3241 ctggtttccc ttggcatgtc cccgcaaacc agcagccgga acacttctgc ttcttttctg
     3301 gaaaaagcag atgccaggct gactgcggct ctgccggtgc gggaactgtt tctgaaaacg
     3361 ttgagcagcg gtgttgttgc caccggcggc aatcgtatcg gggacgtgta cctgacaaat
     3421 gaccggcttc tggaatgccc gcagtcgctg gacacggcgg caatgacagc ttccgcagat
     3481 gccattgacc agttctatca gaagtaccag atccccacga ctgtgattgc cgtaccgccg
     3541 gcaggggagt tctacgcgag cgacctgctg gacggtatgt cctacccgtc gcagatgccg
     3601 gcaatcgaaa gcttctatca ggcgatcgat tcgcctattc gaaaaatcga cgtgtaccat
     3661 gtactgttta ccgccacaga ggactatatc tattaccgga cagatgagaa gtggagctgc
     3721 tacggtgcct actgcgtcta ccgcaacgcc attcagcgaa tgggattcgc tccgatctcc
     3781 tatgaccagt atgtgattac gcacgccggc acatttcggg gaaacttgta tgacagttgc
     3841 ttgtacgacc gcgtaacgcc ggatatgatc gatgtttaca gcgatgagga cggcagcaag
     3901 gtaacggata tgactgcata tccggcaaac ggcgaaccgg aacagcggga actgtatcaa
     3961 acgcagcctg acggcacagc agatttgaac accttttatc tgggagaacc ctgtgagaaa
     4021 ctggtgatcc ggacaaatct gaacaatcag aaaaaactgc tgctgttcaa ggacgattac
     4081 gcagactgta tggtgccgtt tttgttacag cattacagtg agatctgtat actggatgtc
     4141 acccaaatgg aacatggtct gtcagaactg gcagatgtat cagattacac acaggtgctg
     4201 attctctgcg atgcagacac ttttgcggac accaagacag catctgcttt tcgggagggg
     4261 aagaacacat gattgatttg cacatgcaca ccacttgttc cgacggacag ttttcgccgg
     4321 aagaaaccat gcgtatggca cacgaagccg gcgtgacagt tgccgccgtt acagaccacg
     4381 ataccgcatc gggaatttcg cgggcaaggg cagcggcgga gcagtacggc atgacctttt
     4441 tctccggtat cgaaatcagt gtacagggag aaaaggaact gcatattctg ggatatggga
     4501 tccagccgga gaatgcggca ttgcaggcgt tttgcaaaag gcatgctgcc gaccgcaagg
     4561 aacgctgcgg aaaaatgctg gagtatctgc acgggcatgg cgtgcagatc acactggacg
     4621 atgtccgccg ctgcaatgac ggaaagactt ctggcagacc gcattttgca cggacattgg
     4681 tggaactggg ctatgcctcc tcggtgcagg acgcttttga aaaatatctg accacaccgg
     4741 aattctatgc caaagtagaa cgcccgaagc ctacgccgga ggagggaatc ggtgtgattc
     4801 agaatgccgg aggcgtggcg gtgctggctc acccacacca gctgaagctg gaaaacgatg
     4861 cattggaagc aatggtggta cggctgaaag cagcaggctt gcagggcatc gaagcctact
     4921 acagccgcca cacgccgtcg cagacacagt tttatctgca tctggcagaa aagtacgatt
     4981 tgctgtacac ctgcggctct gactatcacg gaccagtcat caaaccggat attgcactgg
     5041 gcactggaat ttccaacaat cttggcattt gtgacgaaaa aattccggaa cggctgtctg
     5101 cggcaatcgc tgcaaaatgt tccgcttgac attttgggcg attcgtatta taatagacag
     5161 agattttgac gatgggaggc agtgcaatgg agtttcatag aaaacattct gtgtgctttt
     5221 tgctgctggg aatgctgttc tgtactgctg gctgcactgc aacagagcag gactccacgg
     5281 cagaaaagcc gaaaaaaatg acagatctgc cgccgttggt aacagagatt tccacagaag
     5341 atacaacggc ggaaatttcc acagaaacca cactggtgac cgaagaagcg gaattgccgg
     5401 aaccggttta tcaggcaaag atgaccgcgt gggaactgcc ggaggaactg ggcagtctgc
     5461 aagcagtacg ggtataccag aacacagtct acctgctggg tgtccggcag aatgcggcac
     5521 agcagtcgga aagcgtactc tattgtgtgt cagaagatgc ggaaagtgca gaaccagtct
     5581 ttacgccgct gtacccaaac agcaatgctt ctgattttgt gggactgaca gatttcgacg
     5641 tgctctctga cggcacgatc tgcgggctgc tctgtgaaaa cgcagatgca gtgccctatg
     5701 aggatccgac attcaacccc gacgatttcg actgggaatc ttattacgaa aactatgcca
     5761 ctcagtatca gctggtatgg tatgacaaaa atggtgtcat ctgcaaaaag ctgggacttt
     5821 ccacactgct ggacctggat gaaacctcca gacagacgat ggcatttacc ggtgtccgca
     5881 gtgacgcttc cgaccacatt tacctgacgg caacgatcga tgaatctgac tgcctgatgg
     5941 cattggacgg caatggtaat ctttgtccgg tacagggcaa tcacaagaat atgcttccgc
     6001 tggaatcaga ttatcagtgg attcggtgca gttcagacgg catgctgctg ttggaatgcg
     6061 atgccgaaga tgtacagcac ctgtcccatg ttgttgtaac agacggggca ctctggaaaa
     6121 cacagacggc tgttccggaa cgcatgaccg cagatacaat gctggcggag tcggaaaatg
     6181 atgactttgt gtacggcatc tggaacgcct ccggtctgtt ccgcatgtcg gcaaaaaatg
     6241 ccctgcnnnn nnnnnnccgg aactgctcta tcggtggagc gatgcccacg tagacttttc
     6301 gaagctggaa cgtgtcatga tgctgccgga cgaggaagtg cttttgtctg catacacggc
     6361 tcagggcaat ttgtcactgg agttgttgga accggatatg aaaaacaagg agccggattc
     6421 taccgagcat gtggaagaag aaaccaccgg cgaaactgtt ccggttgcaa cacctgtaag
     6481 ttaaggcaat accgcacaag ctatttgcgg tgttgcccct tgcaattttc cggtaaatat
     6541 agtatactta taaatgacat tgctgtgcgg aacggacagg ctgcggcaaa atgccgaata
     6601 cgccggcagg atcgaatgca atgagaaaga aggacgaata catgaatatg gaatttatca
     6661 gaaagctgcc gattccgaag gaactgaaag cccagttctc tctgtcggaa aagctggtga
     6721 atgtcaaggc gaagcgtgat gcggagatca aaaaggtgtt taccggtgaa tccgacaaga
     6781 agctgctgat tatcggaccg tgctctgcgg atcgggaaga cgctgttctg gattatgtca
     6841 gccgtctggc aaaggtacag gaaaaggtac aggacaagct gcttctgatc ccgcggatct
     6901 acaccaataa gccccgcacc actggtgaag gctacaaggg catggtgcac cagccggatc
     6961 cggagaaaaa ggaagatatg ctgcagggac tgatcgccat tcgcgaactg cacacccgtg
     7021 ccattgagca gacaggtctg acttgtgcag acgaaatgct ctatccggaa aaccaccggt
     7081 atctgtctga tctgctgtcc tatgttgcaa ttggtgcacg ttcggtagag aaccagcagc
     7141 accgcctgac tgccagcggt ctggatattc cggtgggtat gaagaacccc accggcggcg
     7201 acctgtctgt tatgatgaat tccctgactg cggcacaggc ttctcacgtg ttcctgtatc
     7261 gcggctggga agtcaagtct gcgggaaatc cgctggcaca cgcaattctc cgcggtgcag
     7321 tagacccgct gggcagaacc attcccaact atcactatga agatctgatc gcactgtacg
     7381 aactgtatca gaagaagaat taccagaatc cggctgtgat cgtagatgca aaccatgcca
     7441 attccggcaa gaaatatctg gaacaaatcc gcatctgcaa cgaagtactg cacagctgcc
     7501 gccacagcaa agaaattgct tctctggtaa agggtttcat gatcgaaagc tatatcgaag
     7561 acggagcaca gaagattggc gagggcgttt atggtcggtc gatcacggat ccttgtctgg
     7621 gctgggaaaa gtccgaacgc ctgatttacg atattgcaga tcagctttaa ggcgacaaag
     7681 taccgggcgt tgtactttgt ttcctgcatg agagcgtgtt ccaaaaatgt ggaaaccagc
     7741 tcgaaaagtg aaaggagcga ggtcatatgg catcaatgag tcagttgaca atggcaatgg
     7801 tcacattgta tagcggggat cccaaacgga tacagcattt tatgaaggtg tatgcctttg
     7861 caaaaatgat tgccgaagaa gaacagctgg gagccaaaca gcgggagatc gtagaagctg
     7921 cggcactggt gcatgacatc ggcattcacg cggcagaaaa aaaatatcgt tctgccgcgg
     7981 gagaatatca ggaaatcgaa ggaccaccca ttgcacgggc aatgctgatg gcactggatt
     8041 tttcgccggc actcattaac cgtgtctgcg atatggtggc acatcatcat tcttatgaca
     8101 agatcgacga ggtggatctc cagattctga tcgaagcaga tttcatggta aacgcctatg
     8161 aggacggttt gccgaaacgg aatatcaagt cgtttcggga taagatcttc cggacagaaa
     8221 cgggaatcca ctacctgaac gaattgtttg ctttggagaa ttaaggaaac actgaataaa
     8281 gcttgtgcgt aagatgcgta gtcagatttt tcg
//
LOCUS       DAWBGR010000113         8312 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2325_length_8312_cov_4.340196, whole genome shotgun sequence.
ACCESSION   DAWBGR010000113 DAWBGR010000000
VERSION     DAWBGR010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8312)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2325_length_8312_cov_4.340196"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1942 a         2089 c         2169 g         2112 t
ORIGIN      
        1 gaaaaatttg acgacgcatc tgtcgcacaa tctttgtgcg gtattgcctt agaaacggca
       61 acactgcata aagtttgcct aatggctctg cacacgattt gccgaaaaat ctgacaatac
      121 atctcacgca taagctttat tcagtatttc cctaattata ttataaaaga tttgtcgata
      181 ttctgacatc ttaaatatgt aaaatatatg ttatgttttt gttaagttga acgattacgg
      241 aattaaccag aatttaacgg tacgtttgaa taatttcgct tgtatcaggg aagatctttg
      301 tcgtataata aaatagaatg atactgtaaa tgccagacag ccggtatcgc ttgcaattta
      361 tggatttgat acagggctgg tttaaggagt gaaatcatga aaaaatatgc agtgatcgat
      421 attggatcaa acaccgtgcg tatggtcgtc tatgccgtgg aagaaaacga cacctttcac
      481 gcattgtttt ccaaaaagta tacactggga cttgccggct atgttcagga cggcgtgatg
      541 tcgcaggagg ggattcatag gctttgcggt gtgctgatag aatgccgtat gctgttgtcc
      601 cagtttgaaa tggaacggag ttttgtcttt gccactgctt cgctgcggaa tattcagaac
      661 acgcgggagg cggctgacca gatctttctg atgaccggat tttccgtgga cgtgatctcc
      721 ggacaggcgg aggcatatct ggactattac ggtgtcatgg tagaggcacc gctggaaaac
      781 gggctgctgt ttgacatcgg cgcggcagca cggagctggt gactatggca cacgacggtc
      841 cgggcgtgat tgaaagtctg cctattggct cgctgacgct ggcaaaagaa tacgtgggaa
      901 aggtgttccc caagcagagc gaatgcgaga agatacaggt acggatccgc aaggagttga
      961 agaagcggaa gctgcaccgg ctgccggcac acacgtccct gtgcggcgta ggcggtacgg
     1021 ctcgttcgct gctgctgctg gcacaggaac agcaggaact gccggacacc cagcggcttc
     1081 tgacagcggg acagctgaaa aagctggaaa agctgctctg gaaaaaagac aacaccgcac
     1141 gggaactgct gctgaaaaat tgccccgacc ggctgcatac catttataca ggcatgctga
     1201 ttctggacga gttggtggaa ctttcgcagt gtgaaaccat ttatatcagt cagtacggtg
     1261 tgcgggaggg ctacctccgg cgggaactgg cacggaagcg aaaggcttga gaatacaaaa
     1321 acggctgcat catcttcgcg tgatgcagcc gttttctgtt tctggataag tagttcttat
     1381 tctggataaa tagttaagcg gtttccggaa ccggcagagt ggtttcaatg tgtaccagga
     1441 atttcagcag aacaacggca tcgttgccgg acagttcgcc gttggcatcg cagtctgcat
     1501 tcagagcagc ctgttcgttc agcttgactg tgtcgctgac tgcacgattc aacagaacgg
     1561 catcggtaat gtcaatgcgg gagtccagat tgacatcgcc gtacagaacg ctgctgcctg
     1621 aaccactgcc ggaaacagtg gctgcggtgg tggtagttgt tattgtcgtt gctgtgccct
     1681 tgtcggtggt gccggtcgtg gtaacgtcag tggtttcgga aggcttggta ccgtccggtt
     1741 cttcgcccca gaccagtttg ccgttggcat aagcagggat cttcttggtg attgccgccg
     1801 cgtcgttgtc atcttcaaag ctgatgagat cgctgtagct gtagtcgttg gaggaatccc
     1861 atgtggtgtc ataggtatcc ggattcattt tgttcagcag accgaactga tatacgcggc
     1921 tgccatagaa gttgcagttt tcccatgcga tttctatata gtaggtgccg ttgtcgtcat
     1981 acttgacagg ttctgaaatg gtagcactgg tcttgttgtt ggtcatggca ctttctgcat
     2041 catagtccac acgggtttcc accatggaaa tatcttcgcc aatggcagtc agctcgtcaa
     2101 tgttgaagta gtagcgaacc cgcagatcat tacagaactg cggcggattg atcgtgcggt
     2161 tgtggattac gatcttgacc tgtagaccag ccttgttttc ctgtgccttt ccggcagtga
     2221 catagaagcc ggtaggcgtg ttctcgtcta cttcgtagcc agccttggcg ttttcagaca
     2281 tgttgaagtc cggaatcacg gcgtttttgt ctgccagttc cttgccctct gtgccgtaga
     2341 agtgatacag tcccgcaagg tcgccgcaga agccggcgtt atagtcgtcg gtaacttcat
     2401 tgtaaatgta gtcgctggtg acatcgttgt gatagtcctt cagatccgga ccgccgacca
     2461 gagcacccca cagggtgtgt gtctgcggat cgggttcgtc catgctctgc gtggaggagc
     2521 aggaggacga acggtggtgc gggtgtgtgg cgtagctgtt ttcatagccg acttcaaagg
     2581 aatagcccat ggggttgtcg cccagaatgt attccatctg atcttttgcc cattctgcga
     2641 atgtcaggtc gtcagtttcc ttggcgtata ccattgcaca cagtccagcc gcggtgttgt
     2701 agcgggcact gccgtagtcg gaaactaccg cgaagccagc cggagatttt gcgatccatg
     2761 caccgtcggt ttccggcaga tctttgatct cggaagcggt gatcttggta ttccacagct
     2821 ggtcgtccat gccgaagtat ttgtgcttgg tgaccggtac gtcaaaatct gctttggcag
     2881 catcggcttc acccagaccg gaccagaatt ccagattcca gcggaaaata taccagtcgc
     2941 gggagatgtt ggtgaccgga gccagctttg cgaatacgcc gccccagacg gtatcccagc
     3001 agtgcaccca gatgttctgc cagcagttgc cggtgtcttt gatgatacgc ttcatatagc
     3061 cggtgtacgg gtgagcaccc atttcgccgg tgacagattc gtctacgctg ataatgtcgt
     3121 caatgtagtc atagttttcc gtgcagtagt acagccatac tgctgcccag gacagttcat
     3181 cgtagtcata gctggaggta tagaaaccgc cgtcgtagcc cagactgtct actgtcaggg
     3241 tgtcgtcacc gacttcgtgg tgcgtcttta cggagaaatc gtacagtgcc agagcatagt
     3301 ccagacactt ctgtgcatat tccggatcgg tgtccttgaa gttcagatag ttcaccgcaa
     3361 gggaagctgc tgcaccggcg gtctggtcag aagccggtgt ttccgtggtg gcgaaataag
     3421 ccggacgctt gaccgcacag gaagaagtgg caacataggt gtcgtctacc tgcaattccg
     3481 gagcacacca gtagttgtgg tcgttgttgc cctcacccac ctgatagcag tacgccacca
     3541 cattgccgtc gtcgtccaga aatgtggctt tcataaagta gtcgttgatc cagcggagtt
     3601 cgtcctccac atgcttttgc agaccggttt ccacatagga ttcccggaac tcatagtagc
     3661 cccagcccac agtagaagcg gaatagctgc ccggcagacc gaacttcacg tggtcgccgg
     3721 catcgtgcca gccgccggtc aggtctacgc agccgtcacc gtcggggtcg aggtatttct
     3781 tgttggcatc gatgaagtcc tggctcatgt tggtgcccac gtacagtcct tccgccgcag
     3841 aagcaccgcc gtcaccttta ccgctgtcgt cgttggcttt gccgccgccc agactttgca
     3901 tgggtaccag cggaatcatg ccgtccttga cgtgacagtt gccacgccat gtgtaatagc
     3961 cgtcgtctgc cacttcgtcg ccgcacttgt tggcatcgta gaagcacagt gccagctgta
     4021 gtgcttctgc aaagttgtcg tagttgttgg gattgtcctt gtctactact gccgcactga
     4081 ccggcgaaac agcagcagag gcgatcatga caccgctcag gagtccggca gagattcgcc
     4141 ggagaagttt tgcaatcatt ttacacatcg ttcctttctg ttgccggcac aacacttcat
     4201 attatcatgt gcaccagcat gcttgttgta cattctcaat cacattcttc tggtcgattt
     4261 ttgtggcgac accgcacaaa gtccgtttgc cggctttgtg cagtgctgcc ctatgataac
     4321 cgcaggcaat gcggcaactg cctgcgggaa tatccgttat tctttcggcg gttccggcga
     4381 ttctgtccgg tcgaagatga tctgatagcc gtcacagccg tccttgatgg cacggttgac
     4441 gcggctgatc gtggcagtgc ttgcaccggt ttcctcgacg atttcatgaa agacgtgtcc
     4501 gctgtggagc atttttgcca cctgtaaccg ctgtgccatg gcgttcagtt cctgataagt
     4561 gcagaggtct ttgaaaaagg cgtagcattc ctcaggggaa cgcagcgtga gaatggcttc
     4621 aaagagagct tcctgatctg cattttgtcg ttcttttttc atttttaact tcctttcttc
     4681 gaagtcactg gagcgtgtgt acataaaagc ctgttccgta tttttatgtg cacacactat
     4741 aataccattg taccatatca gccggataat gtaaatactg cacaggaaat ttgtctggat
     4801 ttcggcaaaa ccaccagaaa cggcacctcg tttttgacga attgagaatg ggaaaaacgc
     4861 cgctgctgcg ttgttttgcg gaaatcgtgg caatattctg aaaattccca gcgttttccg
     4921 cgggctttat gcagcgtttc ctttcccctt tgcggtgaaa aacaagcagc acctgtagga
     4981 gatacttccc acaggtgctg cctgctcatt gctacatttt tatgatccaa ccaaatccct
     5041 ttggtcgttg gcgtgagaaa tcatgtcagc aatactccaa tgtcttagat gtcctgcagg
     5101 gcatcatatc cggtttcgcc ggtacggatc tttacaacat cttctacatc ataaacgaag
     5161 atcttgccgt cgccgatctt gccggtgtac agaaccttct tgactgcatc gatgatcttc
     5221 tgcggcggga tctttgccac aaccgcctcg accttgacct tcttgaggag ctttgtgccc
     5281 tcggtcttca ctccacgata gtaggattcg ttgccctttt gagaaccata gcccatgaca
     5341 tgtgttacgg taacaccgtt gacacccata gaggacagtg ttgtctgcaa tgcgtccagt
     5401 ctgtccggat tgcacataac aaccagcttg gtcatctttg ccttggcacc gtttgccggc
     5461 ttcatgatga gttccgactc cggaatggtg acaccagttg cagcagcgtc tgcggcatcg
     5521 gaagccatgg aaacggtaga cggcataaag tctgcatatg cagagggcag attgtgctcg
     5581 gttgcgtcca gacccagaac ttcttcttcg gcggaaacac gcagaccgat cgtcttttta
     5641 atgatgaaga atgtcagtgc aatggtgaca gaagtccatg ccagaatgca gataacaccc
     5701 agagcctgtg tacccagtac ggtgaaaccg ccgccgtaga acagaccgtc ataaacatcg
     5761 tttgccggag cagaagtggt ggcaaacaga ccaactgcca gtgtacccca cagaccgttc
     5821 aggcagtgta ctgcaacggc acctaccgga tcgtccacgt gaagcacgtg atccagcagc
     5881 catacgccga agcataccag cagaccggca acaataccga tgatcgcaga accgaatgca
     5941 tctacgacat cacagccggc ggtgattgca accagaccag ccagcgaagc gttcaggcac
     6001 atggaaacgt ccggcttgcc atacttgatc catgtgaaga tcatgcagga aacggttgcc
     6061 agagccggag caatggtggt ggtcaggaaa atggtgccca gctggggaac cgaggtggca
     6121 gcggcaccgt tgaagccgta ccagccgaac cacagaatga atacgcccag tgcaccgatg
     6181 gtcagggagt gaccggggat cgcattggct ttgactttgc cgtccttggt cttggtgaat
     6241 ttaccgatac ggggtccgag gatcgcagca ccgatcagtg cggaaacacc gccgaccatg
     6301 tgaatgacgc cggaacctgc gaaatcgtgg aacggtgtgg acagctgcga aagccagccg
     6361 ccgccccata cccagtgggt ttcgatcggg tagatcactg ccgagatgac agcggaataa
     6421 atacaataag aaatgaactt tgtacgttct gccatagcac cggaaacaat ggttgctgct
     6481 gttgcacaga ataccagatt gaatacgaag ctggaccagt caaagtttgc atagtccgag
     6541 aaaatgtcaa atcccggctt gccgatgatg ccggctacgt cttctccgca cagcagcccg
     6601 aagccgatga gaatgaatac caccgtgccg aggcaaaagt ccatgaggtt tttcataatg
     6661 atgttgccgg cgttttttgc tctggtgaag cccgcttcta ccattgcaaa gccgcactgc
     6721 ataaagaata ccagtgccgc accgaccagg aaccagatac cgtaaacgtt atcgtttatc
     6781 aggtcaagaa ttgaattttg atccataaga atgcacctct ccgtgatgta atatcggagc
     6841 actatatatg ctctctctgg aagcagcggt accgttctgc ttccatttct tttctgcgat
     6901 tctccttttc ctcacaaaaa agaaaaacag caaaggctct acgcatttct gcacagcgcc
     6961 tttgctgttt gtgttttcag tatagccgat tttgccgcac ttgtcaagcg ttttgcgggg
     7021 atttttgcaa atcaagaaaa aactccgctt ccgctttgga aaacagaaaa aattactggt
     7081 ttttcttttc tccgaaggaa tctgccagaa tctgagctgt ttttgtcaga taagtcacat
     7141 cagactgtgc cagtgagccg gcatcacatg ccgcagctac cttaggatcc agcgggatcc
     7201 gctccagaac cggcacgttg gtgctgtcta ctgcatcatc agaaccgaac aggggaatct
     7261 ccttgccgca gtccggacac ttgatgaaac gcatgttttc caccatgccc agcaccggaa
     7321 tatccatacg ctgtgccatg gaaacggctt tttgcacgat catggaaacc agtgactgcg
     7381 gtgtggaaac aatgatgatg ccgtccagcg gcaggctctg gaagaccgtc aggggcacgt
     7441 caccggtccc cggaggcatg tccacaaaca taaagtccac atcgccccac gaaacttctt
     7501 cccagaactg cttgacaacg ccggagatca ccggaccacg ccaaaagacc ggatcggttt
     7561 cgttttccag cagcaggttc agggacatga ccttggtgcc gtctgctgcg atatagggca
     7621 gcaggttctg ctcatctgtc tgtgcatggt cgtgaatgcc gaagatcttc gggatcgacg
     7681 gaccagtgat gtctgcgtcc agtacagcgg tttcatatcc cagccgccgt gtctggattg
     7741 ccagcaggct ggtgacgagg gattttccca cgccgccttt tccgccgaca acaccgatta
     7801 cctttttcac gccctttgta ttgttgatgg cacgttcatt ctgttctttt tcctgacatc
     7861 cgccgctgga ggcacaggaa ctgcattcgt gattgcaatc tgccatagta tttttcgctc
     7921 cttttggtga ttttccgctg taaaacggta tagggcaata ctgcaccgca gtctgccgga
     7981 aaattcaacg gcatatctta taagctttgt gcggcattgc ctttcttagt ataccacatt
     8041 tttgctccgg ttgcaagtcg gtatgcaaaa gaattgcagt gccttccgac gatgatacgg
     8101 atttttgcct gaaactgaag gaaacactat cctgagctat caaaatcgta caaattcaaa
     8161 ctgttggaaa tatacgatag ttgcacgcgt acccttgaca aaatccgtgg tttttgttaa
     8221 aataaaatca tcaaatcgcg agagtcagag caaaatgctc tatggcaata ccgcacaaag
     8281 attgtgcgac agatgcgtcg tcaaattttt cg
//
LOCUS       DAWBGR010000114         8209 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2353_length_8209_cov_4.144837, whole genome shotgun sequence.
ACCESSION   DAWBGR010000114 DAWBGR010000000
VERSION     DAWBGR010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2353_length_8209_cov_4.144837"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2031 a         2047 c         2369 g         1762 t
ORIGIN      
        1 agcgccacct tgccaaggtg gaggtagcga gttcgagcct cgtaatccgc tccaatggcg
       61 ctatagccaa gtggtaaggc aagggtctgc aacaccctga ttctccagtt caaatctgga
      121 tggcgcctcc aagagatgag aacgcaagcg ggcgttctct ttttttgtat ctcttgaagg
      181 tcaagggcaa taccgcacaa agattgtgcg gtattgccca agctttgcgg ctggaggatg
      241 cgtatacctt agagcctgtc ctgtacctgt tttgcagtgt gcaggacagg ctcttgtagc
      301 acctgaacca atcgaaagga aaggaggaaa tgcaatggca acgtatctga tcgactatga
      361 aaatgtttat atcgagggac tgaccggttt ggagtatctg acaaagcggg acgaggtagt
      421 gattttctac acacagaacc gctgcgggct gacgtttgaa ctgcaccagc ggctgatctc
      481 ctgtgatgca aagctgcggc tgatggaagt gacagcactg tccaccagaa aaaacacggt
      541 gaaaaatgca ctggacttgc agctttccat gtatatcggc tatctgatcg gcagccgtcc
      601 gaaagaacgt ctgtatatca tcagcaagga caccgacttc gacctgaacc agcagttcta
      661 cgaccggtac ctgcaagagg atgtgcacgc tctgtcgatt tattctacca ttgagaatgc
      721 aatgaaaggc attcccacga gagatgcagt acaggctctg gaagaaactg ctgcggctgc
      781 acctgcggat acgacttcaa cagaagaaga aaacgtaccg gcagcaccga aaaaggcaaa
      841 gagcagtgcg gcaatgctgt ttccgaaaga gttacagtcg gaagtgcggg aactgctgga
      901 cacagacgac aagcaggcgg tgaaccggat ctgtgcgatc atcaccgctt cggaagacct
      961 gctgacactg aacaacctgc tgtcccgcta ttaccacgac ggcaagcagg taaaggacat
     1021 gtatcacaaa ctgaagccgt cctataaggc actccgtaag ctggcaaacg gcacgaagtg
     1081 acagactttc aaaacccaaa agagcaccct ccggtgaaac cagccggaga gtgctctttt
     1141 tgcttcataa aaatgaaaaa ccgaaaacaa gcggcaattg cctttgcttt cggtcacttt
     1201 tcatattcaa ctgtcaaaaa cagagcttac gcacgctcga tcttgccgga gcgcaggcat
     1261 ctggagcaaa cgtacatatg acaaggagtt cccttcacaa tcgcttttac acgctttacg
     1321 ttcggcttcc atgttctgtt cgaccgtctg tgggagtgag aaaccttgat accgaaggta
     1381 acaccctttc cgcaaacttc acatttagcc attctactgc acctccttat cctaacattg
     1441 catgccgacc gccggaaaat agacttctgc ggcagtctgc aaactaacat atctatttta
     1501 tcaaatttcc cgaaaaaaag caaccctttt ccttggattc ttttgaatat tttttttacg
     1561 atcaaaatac ctttcgaaaa atacaattat ttgcgaaaac agactgtaaa atcaaatgca
     1621 gacttatttt caaaaagtaa ctgaaaaaga aatcggcaca tttccggcat tctgtcctgc
     1681 ggaaatcttg taaattttat gtcaaacggc aaaataggct tgaatttttc cggaaaacca
     1741 tgtataataa aaagaacaca ttatagggta tctgggtaga acattttcat ttggaaagga
     1801 ctggagcaat gggaattctg agcaatttta tgaatttgaa ccgttatgag attctggcaa
     1861 gaatcgtgat tatattgttg gtgttgccgc tgcatgagtt tgctcatggc tgggtggcac
     1921 ggaaatgcgg tgatgacacc gccgaagctg ccggacggct gacgctgaat ccgctggcac
     1981 acgtagaccc ctttggtgca gtgttgttgc tgctgaccgg attcggttgg gcaaagccgg
     2041 tgccgatcaa tccggcacga atgaaccacc cccgcaaggg catcatctgg acatcactgg
     2101 cggggccgct gtccaatctg ctggcggcac tggtgggcgt ggtgctgatg cagatcgtct
     2161 ttccgtttta ctatgccgac cccaacgccg ttttgacagg catctatacg ctgctgtact
     2221 ggttcgtggc gatcaacatt tcgctggcag tgttcaacct gattcccgtg ccgccgctgg
     2281 acggctccaa ggtgctgatg atgctgctgc cgtaccgcca gtccgcgtgg cttgagcgaa
     2341 accagcggta tttgtcgctg gcactgtgga tcctgattct ggtgggcgta ctgagcacac
     2401 cgctgagtta tctgtccaac tggatcatgc acttcttcgt ctgggcgacc cagtggatcg
     2461 gcacgctggt gcagatggtg atgtgagttc atgcagacga tttcgtttaa gctggaggtc
     2521 tttgaaggcc ccatggatct gctgctgaac ctgatttcca agcacaagct gaacatctat
     2581 gacattgaga tctccagcct gctggaacag tatctggcgt atctggatca gtgtcgggaa
     2641 caggacctgg agctggcagg ggaatttctg gaaatggcgg cacggctgat ctacatcaaa
     2701 acggcttcgc tgctgccccg tccggaagat gcccaaaagg aaaagcaggc attacagggg
     2761 gcactgatcg agtacgccct ctgtaagcag gcggcacagg ctctggcaca gcgttttgtg
     2821 ggcggacaga tcttcacccg tccgccgatg caggtggaag tttctccggc atacgccaga
     2881 acccacctgc cctgtgtgct gctggaaaca tggaacacca tggggcagaa acgctttgcc
     2941 ccgcaggaaa acgcccgcga acggttacag cgtgtagtga cgcgtccggt ggtgtcggtg
     3001 atgtccaagg tggtctacgt gatgcgaaag gtgtatcacg atgaacacgt gtatttcggg
     3061 gcactgtacg ccgatgtgcg ggagcgttct gcacgggtgg cactgtttct ggcggtgctg
     3121 gaactgacca agcatggcag gatcggcatc agcgaggacg gcacctatct ctatcgccgg
     3181 acagactatg tgaaacgccc tgcaaagggt taagtcaatc ccgcgttttg atttgaatca
     3241 agtatgaatc cgaagaatcg gggaatgata atggaaagaa acaccagatc gccgctttgc
     3301 ctgatgctgg aggcggtgct gtttgccagc ggagagccgg tgccggtgtc ccgtctggca
     3361 caggcttgtg aagtatcaga atctgcggtg cagactgcct tacagcagct ggcggcgtgg
     3421 tatgaaaaac aaaaaagtgc gatctgcgtg cagcagctgg gggacagctg gcagatgtgc
     3481 acccaccctg cctatgcgac tgtgatccag cgggcagtgg aaaccaaaaa ggcggctccg
     3541 ctgtcggcgg ctgccatgga ggtgctgacg atcgtggcat ataaccagcc ggtcagcaaa
     3601 agctttgtgg agcatgtccg cggcgtggac agcagttccg tggtgaacac cctcgtggaa
     3661 aaggaactgc tcacagaagc cggacggctg gacgtgccgg gcaagccggt cgcctatgcg
     3721 acaacgcccc attttctccg gtgcttcggc ttgcagtcgc tggacgagct gccgccgctg
     3781 ccggtgtcgg ctgtgccgca ggaaatggta ccggagtcgg aggaatctga caatctggca
     3841 gaatgatgca gaggaggcga gtggtctatg gttggctggc tgatcgtgct ggggattctg
     3901 gcactgctgc tgggactgct gttgtcgccg ttctcctttg cgtgggacta cgatgccccg
     3961 aaagaccgct ttttctggaa gctttcgtgg ctggggatct cctttgctga cagtgccgga
     4021 aagggattgt ttcagcggga aaaagaggaa tcccggaagc cggaaaaaga accggaaaag
     4081 cagaagaaaa agaagcggaa gaagccgaaa aagtcccgcc gaccggcaaa aaagaagtcg
     4141 ccggaatcat cggaaaagac cgaaaagcaa aagcttgaac acggctgggc tctcatcaag
     4201 ggcagcctgt ccgtgcttcc gaaaccgttg cggctgctgt ggaaaggcat ctctctgaag
     4261 cagctgacag tgggcgtgca ggtgggacgg ttcgatgcac aggaatgtgc cctcgcctac
     4321 ggagcagcca atgcggcagt gtacaccgct gtggggctgc tgggcagtgc catgcgggta
     4381 caggtgcagc agatacaggt gcagtgtgcg ttcgggcagg agtgctcccg ctgggtcgtc
     4441 cgcggaaagc tgcacctttg ttttctggca gctctggcgg cactgtgcag ctttgccgta
     4501 ggctatgcga tgcagaaaaa ccggtaggaa actgacggga aagagaaaga agataccgca
     4561 gaaaaagaac acgctgcggt gagataacag gaggtaaatg taatggcaga aaagcgtccg
     4621 attcaggatc tcatgggagt tacaatggaa aaaatgcggg aaatggtaga tgtgcagacc
     4681 attatcggag atccgatcgt ggtttctgac aaggtgacca ttgtgcccgt gtcaaaagtg
     4741 tcatacggat ttgcctccgg cggctctgac ctgccggtga aaaccaatcc gaaagacctg
     4801 ttcggcggcg gtgccggtgc cggcgtgtcc attcagccgg tcgcctttct ggtggtacag
     4861 gaggacggcg tgcggctgct gcaaatggac gagggcagcg atgctctgtc cagtgccatt
     4921 cgctcagtgc cggaagtggt agaccgtctg ggcagcatgt tcaagaaggg cgacaagaaa
     4981 aacgacaaaa agactgaaaa tccggaaaaa cctgcggaaa cggattccgg cgaagcagaa
     5041 taaactgccg cttctttcgc atacattgtg gtaattttac aagtatgcgg aggaatctga
     5101 aatgcgaaaa caacattttt tgcggtggct cagcgggata tgggcggtgt gtctggcggt
     5161 gactggtctg ccggtgctgc atgtacaggg cgaggctctg tcggtttcgg cggagtcctg
     5221 cattctgatg gaagcggtga ccggtgaggt gctctatgaa aaggcggcag atcagaagcg
     5281 acctatggca agcacgacca agatcatgac cacgctgctc tgtctggaaa gcggcgatct
     5341 ggatacgccc tttccggtgg acaacgatgc gatccatgta gagggctctt ccatggggct
     5401 ggtagacggg gacattgtga ccaagcgtgc cctctgctat ggcatgctgc tgccctccgg
     5461 caatgatgct gccggagcaa ctgctgtgaa gcttgccggc agctatgccg cttttgcaga
     5521 gcagatgaac cggaaggctc aggaactggg catgacccag agccattttg tcacgccctc
     5581 cggtctgcac gatgcggagc attacgcaac tgcccatgat atggcactgc tgacagcggc
     5641 ggctttgcag aacgaaacct ttcgggagat ctgcgggcag gcttctgcca aggtgtgctt
     5701 cggcaatccg ccctatgaac ggtggctgac caattccaac aagctgctga ccatggacga
     5761 aacggtgatc ggtgtcaaga ccggtttcac agacgaagcc ggccgctgtc tggtgtccgc
     5821 cagtgtgcgg aacggcatca cgctgatttg cgtgaccctg aacgaccgga acgactggca
     5881 ggatcatgaa gtcctgtacg attacggctt cgggcagctt tccccgcagg aggtgacatt
     5941 tcccgccgac tggacgctgc ccgtgaccgg cggcaccaca ggaactgctg cggtggctcc
     6001 tgcggaaatg ctgacagtag gcgtgaaaga cgggaacata ccggcgtggt cgtatcagct
     6061 gaacacgccg ccgttcctct atgccccgct gaccgccgga caggaagccg gcacgtggaa
     6121 gcttctgctg gacggcagag ttgccgcaca gggcaggctg ctggtgcagt ctgcggtaac
     6181 ttatcaggaa aaaacgacag agccggaacc ccaaagctgg tggaaacggc tgctgcaaaa
     6241 atagagcaaa aaaggagtca aacaggaagt ggaaaaaata cgaattcaaa aactgctggc
     6301 agatgccgga tattgttcca gacgacaggc ggaaaagctg atctctgacg gaaaagtcaa
     6361 ggtgaacgga caccctgtca agctgggcga taaggcaaca ctggcagatc tgatcaccgt
     6421 aggcggcgaa cgggtatatc tgccgcgaaa gaaacagtac cgttatctga tgctgtataa
     6481 gccgcggggc tatgtgacca ccacctctga cgagctgaac cgccgctgtg tcatggatct
     6541 gctggaggac gtggaggaac gcgtttaccc cgtgggcaga ctggacaaag attccgaggg
     6601 cttgctgctg ctgaccaatg acggcagctt cgccaacggc atcatgcacc ccagccgcca
     6661 tatcagcaag acctatcgcg ttaccgtccg tccggacgtg accgaggaac agctgatcgc
     6721 cctgtccggc agcatggaac tggacggcaa aatgactcag ccggcaaccg tggttgtcaa
     6781 gaccaaggag ccggggcgtg tcgtcctgct gatgaccatt catgagggca agaaccgaca
     6841 gatccgccgt atgtgtgaat ccatcgggct ggaagttgcc cgcctgcggc gaatcagcat
     6901 cggaccgctg aagctgggca tgctgaagcc gggtacttac cgtgacctca ccgccgagga
     6961 actccgtgcc attcgaaatg ccatgggaac cggcgaaacc gctgctccgg caaagaacaa
     7021 gggcagacgg agataatcgg agtattcata gttctatcga taattctatc aaaaaacgtt
     7081 ctgggaggag gaacaggcaa tgccaatttg tatttcaccg gagctgccgg cgtatcacat
     7141 tctgtccgag gagaatattt ttgtcatgga cagcgaccgt gcggctcatc aggacattcg
     7201 cccgctgcga gtgctggtgc tgaatctgat gccgaaaaaa attgaaacgg aatgtcagtt
     7261 cctgcggctg ctcagcaaca cgccgttgca ggtgaatgtt gaatttctgc atgtatccag
     7321 tcatatcagc aagaacacgt cgcagaatca ccttgccaca ttttatcgga ctttttccga
     7381 aattcaggat cagtattacg acggctgcat tatcacaggt gctcctgtag agcatctggc
     7441 atttgaagat gtggactact ggcaggagat ctgcacgatc atggagtgga caaagtccca
     7501 cgtcttttcc acgctgcaca tctgctgggc ggctcttgcg gggctgtatt atcattttga
     7561 tgtgcccaag tatgacctgc cgcagaagat gttcggcgtt ttcccgcagc atgtgctgga
     7621 gcctcactgt cagctgctcc gcggctttaa cgacgtgttc cacgcaccgc attcccgtca
     7681 cgcggaagtg catcgggagg acattgaaaa ggtgccgcag ctgaaaattc tgacggagtc
     7741 ggaactctcc ggcgtgcaca tcgtcgccaa caaaaacggc agacagtact tcatcaccgg
     7801 tcattccgag tatgatcggg acacgctgaa aggggagtat tttcgggaca aggaaaaggg
     7861 agcagagatc gcggttccct atggctattt tccagatgat gatcccacca aggaacccca
     7921 tttcagctgg gggtgcagtg cgaacctgct gttctccaac tggctgaatc actgcgtcta
     7981 ccagcaaact ccttacgacc tgactgcact ggaactgtac aactgggact ggcaggcagg
     8041 actgtaaggc atcatattgt acaagctgtg aaatcataaa aaacccgtcc gcaccggaga
     8101 gccatggtgc ggacgggttt ttagtttcaa ataaatttag atagtaaaaa caaatttagg
     8161 gaaacactga ataaagcttg tgcgtaagat gcgtagtcag atttttcgg
//
LOCUS       DAWBGR010000115         8179 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2358_length_8179_cov_4.962088, whole genome shotgun sequence.
ACCESSION   DAWBGR010000115 DAWBGR010000000
VERSION     DAWBGR010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8179)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2358_length_8179_cov_4.962088"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2005 a         1991 c         2293 g         1890 t
ORIGIN      
        1 gttttctgcc gtgctccgga aagaacgtgt ttttggcggc ggcttcgccg aaaaatttct
       61 tgaggaaagg aaacatggat tgatgaatat ctggcatgat ataaatccca agcggattac
      121 agtggacgag tttgtggctg tggtggagat cccgaagaac agcaaggtca aatacgaact
      181 gaacaaggac accggcattc tggaaatgga ccggatcctg tacacttcca cccattatcc
      241 ggcaaattat ggctttatcc cccgcaccta tgcagatgac ggtgacccgc tggacgtgct
      301 ggtgctctgt tctcagacgc tgcaccctat gacactggtg cggagctatc ccattggcgt
      361 gatccatatg atcgataacg ggcagcggga cgacaagatt gtcgcgattc cgtgtaatga
      421 cccgacatat aacagctatc acaacatcga cgatctgccc cgtcatattt ttgaggagat
      481 cgtgcacttc tttcaggtgt acaaagagct ggaacataag acgaccgcgg tggacgaagt
      541 ggaagacgtt tacgcagcaa aggagatcat tgcggcagac atcgaccact atatcgataa
      601 gttttgtaag gcttgaccgg cacggggaaa agtaatcaat ggtgcgtttc tccgtccaga
      661 tcggcggtta ggtgtgccta aatatagaaa gaggtatttg agttatggta tcagcatcag
      721 acattttgtt caatcacgga accagcgttg caccgctcgg actgattgca atgaaaggtg
      781 cagaggaact gggcaataag ctgaatgact acttggtaaa ctgggctcac aagggtggtt
      841 atgatgtaga cacattcctg atcgaatcgg aatgcccgcg tttttcttcc ggtgacggca
      901 agggactgat caagtccact gtccgcggta aggatctgtt cattattgtg gacgtaggca
      961 actacaacgt gaaatatcag atgtacgatc agatgaacgc aatgtcgccg gacgaccact
     1021 atcaggatct gaagcgtctg attcaggcag ccagcggtaa ggcacaccgt atcaacgtga
     1081 tcatgccgat cctgtacggc ggccgtcagc accgccggaa ctaccgtgag tctctggact
     1141 gtgcctgtgc actgcaggaa ctgtatcaga tgggcgtgtc caacattctg actgtagatg
     1201 cacacgaccc ccgcgtacac aatgccattc cgctgatggg ctttgacaac atgatgccgt
     1261 cctatcaggt gctgaaggca atgttcaaga cgatcccgca tctcaagaca acaccggacg
     1321 agttcatggt catcagtccg gacgagggtg ctctgaaccg gaacatgtac tacgcttcta
     1381 tgctgggcgt accgatgggt atgttctaca agcgtcgtga ctattccaag atcatcaacg
     1441 gacataaccc gatcgtagca cacgaatatc tgggcaactc cgtagaaggc aaggacgtat
     1501 ttattgccga cgatattatt tcctccggcg aatctatgct ggatattgcc tacgctctga
     1561 aggaacgcaa ggctcgccgc gtgttctgct atgcaactta cggtctgttc gtcaacggtc
     1621 tggaaaagtt cgacaaggca aacgaagacg gctatatcga tgctgtattc tccaccaacc
     1681 tgacttaccg caccgatgct ctgctgtccc gtgagtggtt ccacgaagta gactgctcca
     1741 agtacatttc ctacttcatc gcatctctga accacgatgt ttccatcggc aatgtcattg
     1801 acccgaacgc aaagatcaag gcactgctgg aacgcagaaa caaggaaaac gcataaggca
     1861 actccgcctg acggctttgc cataagtttt gataaataca ggagaacatg ctcctagggc
     1921 aacaccgcac aaagcccgcc tgacggcttt gcacgcaatc tgcgtgcaga ttttccgtca
     1981 tattgcccag aaatcccttg ccatacggca gtatgcctgc ggaatttcta ggcaatctgc
     2041 cgaaaaatct gacaacgcac cttacgcaca agctttgtgc ggtgttgccc tagaaaaact
     2101 gctgtgccgt ttttgccggt gcggcagttt tttctgtctg tttgcgaaaa aaacagttga
     2161 caaacagcac gagatttgct atactaataa tgatgttgat ataaaatggg cgaggaacgg
     2221 aataactgtc agaacagccg tgacagagag agcaggtctg gtgaaactgc tccggtatgt
     2281 ggacaggtgc tacccgcgag cctttccgga aaaccggaac gtatttcctg cgttagagga
     2341 aaagagggaa atgcaaattt ccgaacttgg gtggtaacac gaaggccttc gtcccatttg
     2401 gcggcgacgg tctttttttg tgctctctgc gactgcggag agatgcgaca acaccgtatt
     2461 tttcaaggaa aaaggagtct gaaaaccaat gaagtggcaa ggtgtaaacg aattacgtga
     2521 aaagtatttg tcgttttttg agagcaaggg acatacccgt ctgcccagct tttcgctggt
     2581 accgcagggg gataagagcc tgctgctgat caattccggt atggcaccgc tgaaaaaata
     2641 ttttctggga caggcagttc cgccgaatac ccgtgtgacg acctgtcaga agtgtatccg
     2701 tacaccggat attgaacgtg tcggtcagac cgcacgtcac ggcacctatt tcgagatgct
     2761 gggtaacttc tctttcggcg attactttaa gatcgaagca atcacatggg catgggaatt
     2821 tttcaccaag gtgctggaaa tgccggcaga taagctgtat gtttctgttt tcgaaaatga
     2881 cgacgaagcc tatgacatgt ggaccaagca gatcggcgtg gcagaagacc acatggttcg
     2941 tctgggcaga gaagataact tctgggaaca cggttccggc ccctgcggtc cgtgttcgga
     3001 gatctacttt gaccgtggac cggaaaaggg ctgcggcaag ccggactgcc acgttggctg
     3061 cgactgcgac cgctatgtag aagtatggaa tctggtattc agccagttcg acagtgacgg
     3121 caacggtcac tataccgaaa tggaacacaa gaacatcgat actggtatgg gtctggaacg
     3181 tctggcatgt gtcatgcagg gcgtagacaa cctgttcgaa gtagataccg tgcagaatat
     3241 catgaagcac gtctgccgca tcgccggtgt tacctataac gcagacgaaa agaccgatgt
     3301 ggctctccgc gtgatcaccg accatatccg cagcaccaca ttcatgatcg gtgacggtgt
     3361 aatgccgtcc aacgcaggcc gtggctacgt gcttcgccgc ctgctccgcc gtgccgcaag
     3421 atacggcaga ctgatcggca tcaagaagcc gttcctgtat gaagtggtag aaactgttat
     3481 tcacgaaaac gcttccgcat atccggagct ggaagaaaag aaagcttata tccgcaagat
     3541 cgtggaggaa gaagaaaaga ctttcgccaa gactgtggac aagggcttcg atatgctgtc
     3601 cggcattctg acacagctga aagcagaggg caaggatacc gtttccggcg aagatgcatt
     3661 caagctgtcg gatacctacg gcttccccat tgacttgacg gaagaaatcg ctgcggagca
     3721 gggcgtatcg gtagaccgtg aacgctttgc tgcactggta gaagaacagc ggaatcgtgc
     3781 aagagaggac taccacaata aggcaggttc ttcttgggca gaaggcggtc tgcaaatgga
     3841 tgctccggca acggttttca ccggttatag tcacaccgct gaagaagatg ccaaggtcgt
     3901 tgccattttc cgtgacggcg aatcggtaga cactgctgcg gagggtgaac aggctgtagt
     3961 tgttctggat aagacaccgt tctacgcaga aagcggcgga cagatcggcg attccggttc
     4021 tgttaccaca ccggacagca ctttcaccgt agaaactacc agcaagacag aaaacggtca
     4081 ctatctgcat ctgggtgcta tgacagaggg tgcactccat gtaggtgata ccgtttctgc
     4141 aaagatcgat gcagacaaac gcagcagaac catgcggaac cacactgcgg cacacttgct
     4201 gcaagctgct ctgcggagcg ttctgggcga ccatgtacat caggctggtc agctggtaac
     4261 cgacagccgc tgccgttttg acttctcgca ctttgcagca atgacacatg aggagatcaa
     4321 gcaggcagag gctctggtca acgaaaagat tctggaagca attccggtcg tgacacagga
     4381 actgcccatt gccgaagcca gaaagctggg agcaatggct ctgttcggcg aaaagtacgg
     4441 cgatgttgtt cgcgttgtta ctgtaggcga ttattctgta gaattctgcg gcggtacgca
     4501 tgtggataat accgcaaagc tgggtctgtt ccgcattctg tccgagagtt ccgttgcctc
     4561 tggtgtccgc cgtatcgaag cagttaccgg caccggcgta ctggaactgc tggattccta
     4621 tgagcagacc atggcttctg ctgcaaaggc aatgaagctg agcaacacca ccggactggc
     4681 agaacactgt gcagcactgg tggcagacaa taaggagaag gatcgtcaga tcgaatccct
     4741 gaaccagaag atctccgatg cacagatggc tgctattttc gacaatgcca aggctgtcaa
     4801 tggtgtcaag gtggtttccg caatgctcaa tggcactaca ccgcaggtac tgcggaagat
     4861 gggcgacacc attaaggata tgaaggaatc ggtcgttgca gttctcgcaa gcgtcaatgg
     4921 cgaaaagggt aatctggttt gtgcatgctc tgctgctgcc cgtgataagg gtgctcacgc
     4981 cggcaagatc gtacagcgtg ttgcagcagt taccggcggc aagggcggcg gtaagccgga
     5041 cagtgcaatg gctggtatcg gcaagaccta tatggtagac gaagcactgc tggcactgga
     5101 cggcattgct gcggaattcc tgaaggagga ctaatcatgg aagattgcct gttttgtaag
     5161 atcattgcgg gagagatccc cagcacaaag gtctatgaag atgacactgt gtatgtgttc
     5221 aaagatatta acccgatcac tcccattcac tatttgctga tcccgaagca gcacatctgc
     5281 tgtgccgcaa agatcacgcc ggaaaattcc gcagtcgtag ggcacatcta tgaagtggct
     5341 gccaagatcg cggaaaaaga gggcatcaca gacggctacc gcattgtgac caactgcggc
     5401 gaaaatgctg gtcagaccgt gttccacctg catttccata tgcttgccgg tgtgaaaatg
     5461 ggctggggtg ctgacgcagt acagccggta gaagaatgag gaggtgctgt ctgtggcagc
     5521 gtattataca ttgcacattg ccggtctgac acgggaactg aagatctgcc cgatcagtga
     5581 aaagctggat attgcggcgt ttattctatt ttccgatgtg gaactgacag aagcgtgtgc
     5641 cagggaactg gcaaagcgtg tgccggaatg tgactacatt ctgacggcag aatccaaagg
     5701 cattccgctg gcatacgaaa tgtcccgtca gctgaaagtg ccgtatatcg tagcacgaaa
     5761 gtccgtcaag ggctacatga ctgacgtgct gtcggtacag gtgaaatcca ttaccaccgc
     5821 agccgtgcag accttgtatc tggacggcga aaaggcggca tggatgaagg gcaagcgtgt
     5881 gctgatcgtc gatgatgtca tcagtaccgg cgattccctc cgtgctctcc agcagctggt
     5941 aaagtccgcc ggcggagaga tcgtgggcaa cgcagcacct ctcgcagagg gtgatgctgc
     6001 ggaacgcaag gatttgattt atctggaaca attgccgttg ttcttcaagg attgaggaca
     6061 aggttgagat gaaacggaaa atggtatggt ttgggattcc atggctggcg gggttattcc
     6121 ttgccacagc ctgccagact tccgtgacgg tgagtctgct tctggcggca tttctgctgc
     6181 tgggagcatt ccgtctatat cggcgtatca ccacggggca gctgctctgt gtgggacttt
     6241 ccgcggcggc agccgcagga gcggtgctgc tgtatactac tgccgtgtac cagccgcttc
     6301 tgcgatctgc cggaaccata actacatttt ccggcagagt tttcgcggca aaagtctacg
     6361 ataacgatcg tgcgtcctat caggtaaaag gcacgtttgc ggacggcagg cgggcgaaaa
     6421 ttctggtata taccgacgat gttggtgcac gatacgggga catgctggac gttgccggcg
     6481 gattctccgc actggagaac agctacctct ggaacgggga gagttactac cgagccaagg
     6541 ggattttcct acaggcgaac aatgacgcgt tcgtgtcctg tacgccgacg gaaaacggaa
     6601 agctggtgcg ggcattgcag caataccgcg accgcatttc tgtgcggatc tgtacccttg
     6661 ccggaacgga agccggcggt atggtcagtg ctatgctgct gggaacaaaa gatacccttg
     6721 ccgatgaaac gaacgacttg ctgacacatc acggaatccg ccacgtggta tctgtttccg
     6781 gtctgcatct ggtactgatc ttgtccattt ggggctggtt ttgcaggcgg ttccatatgc
     6841 accgctgggc agtgttcggc acaaccatag tctggaccgt gctctatgcc ctcatggtgg
     6901 gaacgccgat ctccattctg cgagccggtt tcatgtatct gctgatgcag agtgcaccgc
     6961 tgtttttccg gcggggtgat acctgtaatt cgctgtgtat tgccggcgtg ctgatgacga
     7021 tcgggaaccc atatctgata caggacgcat cttttctgct gtcctttgtc ggaacctttg
     7081 gcattggcat gtttgctccg tggctgacca aacgcctgcg gaaaaaaggg ctggaatgga
     7141 gcgtgctccg gcagcttgcc acagtgacca gcgtttccgt ctgcgtgttt ccggtgacgc
     7201 tcctctattt tcgggaagtg tctgtggcat cgcccattgc aaacctgttt ttggtaccca
     7261 tttgttccgc gatgctggca ttgtcgctgt tgatttttct caccggcggt gtgggatttg
     7321 tggcgaaacc agtttgcttt ctgctgaagc tgctgtatga cctcttaaaa ctcatggcgt
     7381 acggactgcg ggatttcatt ccgtttttgt ttcccaccgg ctggaagctg cttcccatgc
     7441 tgtgcggcat tctgctgttg ttcgtgttgc tgattttcta caccaaacgg agccggaaag
     7501 ccgtgtcgct ggcgattgtc atttcctttg gaatcctgct ggcagggcag gcagtctatc
     7561 gggcaagcga acagcgttgc tttctggtca cagttctggg gcagaagcag gaggaagtgg
     7621 tagtcgtcac ctatgccgga aaaaccgatg tgatcgacct gaccggcgac caccggaacc
     7681 cgaaatatgt ggaggcatac tgtgccgaac agggcattac ccgcttgaat acgctgtgcc
     7741 tgacgaaata tgcagaccag atgcgaatcg cctatccgga caccctgtgg gaaatgccgg
     7801 tggcagaaac cgcggtgccg gcagactgct ggattcccgc tgatgcagat tttatgggaa
     7861 caaagccgct gcaaacggac aagtttacag tggaggatac ccagtacact ctgacagaat
     7921 ctgacggtat cgtctggatt acattcggga atctgcattt ctgcgtgggc acgaccatac
     7981 aggaactgcc cgataccgca gatgcggtga tctgtacccg gtggaatgat gaaccagatg
     8041 caatgccgga ggaaacctac gccaaggagg acgcggtgca gatacgggta tatcctgacg
     8101 gcagctggaa tgtgaataca ttagggaaac actgaataaa gcttgtgcgt aagatgcgta
     8161 gtcagatttt tcggcagat
//
LOCUS       DAWBGR010000116         8053 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2392_length_8053_cov_2.854089, whole genome shotgun sequence.
ACCESSION   DAWBGR010000116 DAWBGR010000000
VERSION     DAWBGR010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8053)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8053)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2392_length_8053_cov_2.854089"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2002..2011
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2516 a         1561 c         1694 g         2272 t
ORIGIN      
        1 caagataatc atcaatcatc tgacttgcct tgtccagatt agcatactct tttttgagat
       61 agcggtattc ttcataaagt ttttcccgtt cctcatacaa tgcctgcctt tggtggtcaa
      121 gcgccatttc tgacgggaat ctgccgtccg gatacaataa ttttaactga cttccaacgc
      181 tcttgtactt ttccagtaca ggagcgtttt tctttgcata atctttttgt ttccatgctg
      241 aaagggactt gtacttgtca tgatacggtt tactgcgttg aaattgtcgg acaagttcta
      301 tctgttctga caaagtgttt atctttttat ccagattttc aatgctctgc gtaatcattc
      361 cacgccgtgc cataacagtc atggtggtcg gtttcaattc cgatgtgctg tgaacattgt
      421 aactttcaag caaatttatc atctgagcta cgttcttcat attctgaata tccgcccaat
      481 actgcaagcc cctgttatca aatttactac ggtcaagaat catcttcctg tgtgatgaaa
      541 actgtctgat tctacgctga atattctcag gaagatagta atatcccagt gtttttgctc
      601 tggcaaagcg ttgttgtccc tgtatacgaa atttaagaga aaccgcattt tcaggtttgt
      661 aaactgcttc gattccgttc aatctgcact tttcaagaaa gtcctcaaat gtatcagacc
      721 gctgaatgat acagtccagt tcatagcgaa attttgcctt ccatgataaa ccctgctgct
      781 gttttgtcca ttcatagtgc gaaacacctt tgctgatttc cccgtatgca atcacagaca
      841 gtcccatttc tttacacacc tcgtcagaaa actgacggat tttcttccat gcaggattat
      901 gtttggtatc catgagtgta ccaaacgttt tgtaattttc catattggtt ttgcagaaaa
      961 taatgtgatt atggatatgg tctttgtccg tatgcgtggt aagaacatat tgatatttac
     1021 cctgaaagag tttgtcgcag agcatcagtc cagcctgatg cacctgttcc gctgttgctt
     1081 cacttggtgc aaaactctgt atgatatgat aactgagaac acttcctgta tttcttccca
     1141 tacctctcag cttgctgaat tcgtcagaaa tctcagctgc atttccccag cagccgtatc
     1201 gggtaacata tgctgctttc tcaggattca tgatatactt gatggattta gcttctgtaa
     1261 ctcggattgg atggattgaa gttgttgcca tatctcagtc actccctcct gcaactcata
     1321 aagttcttgt ttgtaaatac ggcttgtgga gttcactctg tgtgcaatct gattgacgtt
     1381 gtttgccaca cgcataaccg cctgccgtat ttttttcagt tcctcatggt cgtattcgag
     1441 atagattcca tatagcatca tctgtctgaa tagatcgctc atgtttgaaa cgttacattt
     1501 ttcaagacgt ttctgtatga tattccactc atcttcattg accttgaaga tgagattctt
     1561 gtcacgcttt tttctgttca ttgttcaaat ctcactttct tatgtctatg ctgttgttga
     1621 ttttcgttgc gtcagcaacg ttccaaaagg gtgttaggga aaacgaagtg tccctaacaa
     1681 gctgatgaag atttaacgga cgttaatatc ttcatcgttg cttgctactc cagatcagct
     1741 gtgttagtcc atttttcaat ctgttcagat ttcccctcac tctatatggc aaatatcatg
     1801 caaaaccgca ccgctataat tgtaaagttt acaattattt cataataaaa atctgaaaac
     1861 acgttcacga tacagttttt tgtatcctag gtgtgctata atatctcaaa ccaaaacctt
     1921 catgtgcaca caagaagttg gttatattaa aacaacctac tttttttatg gaggtcttta
     1981 tgaaagacaa cttacgttat annnnnnnnn ngagatgtac tcacttgtga aaagcagaca
     2041 ggtcgagcgg attctcgtca aggacttatc ccgtctggga agaaactata ttgaaacagg
     2101 tcgatttatt gaccttgaat ttccacgtta taacacgcag ttcatcgctg tgaatgaaaa
     2161 ctttgacagc aatggcggcg acagatttct tgttgcagta tggaatctgc tgaatgaact
     2221 ctatgcaatg gatatttcca acaaacagaa gcagtccatt cgggcaaaaa gtgacagtgg
     2281 caggcacatc acctcaaaga ttccatttgg ttacaaactt gatccggaag ataagcacaa
     2341 atggattatt gatgagcctg ctgctgaaac agtcagagtg atattccgtt tgtttaacgc
     2401 aggcaaaggc actactgaaa tatgcagata tcttaccgag cataagttca tatctccgtc
     2461 caatcataac aagcaggcaa tcaaaggttc tcgtgctgtt gcagacccat actattggtg
     2521 tgtcagcagt gtgatcaata tccttgaccg tcaggaatac gttggtgata cggtaaattt
     2581 caaaacatat catacaagct ttaaggataa aaaagtccgt aaaaacagcc gtgaggatta
     2641 cgtcattatt catgatacac aagaaccgat catctcccgt gaggagtttg aaaaagcccg
     2701 gaaaagaaga aacgccaata agagaatttc aactgaaaaa caatttcatc tgcttgacgg
     2761 tatggtattc tgtggcgatt gtaaaaacag aatgtacctc aatcgcaaga aaagaaaatc
     2821 aaccgttaca tacatctatc tctgcaatcg gtataaaaaa gaaaagcttt gttctgcaca
     2881 ctacattcgt gaggactatc tgatcaaaac tgttctggat cagattgctt tcctgcaaga
     2941 gaaattttca gagggaaaaa ttgagttccg ccgtttcatt tacagtgcga tcaatcatcg
     3001 tacatctgaa catatcagac aggttaactc acggatcaaa gcaattcagg aaagactgaa
     3061 tcaaatcaga acgacagaaa ccaagatgta tgaagataag cttacgggca aagttgacga
     3121 ggaaacattt cataacatca ctcatgttct gaacaatgaa gcaagaaatc tgacagatga
     3181 aaattctcag ctgcttgtca ttcttgacaa agtggaggat ctgaaaatgg gcattgataa
     3241 ctttgtacag aagatcgaac gttttgcaaa ctgcacagtt acagaaaatg accgtgtcat
     3301 aatggaacag cttatcgatc atattgaggt ctatgaaaat gacagcaatg aaatttctgt
     3361 gcgtatcttc tttgcagaca tcggcgtaat tgaataagct gcactttgat tacaagtgcg
     3421 acggtatggc gacgatgtat taaaactttg gtgacaatgt tcccataact attgaaaaaa
     3481 gtccttctgt gcgctattct aaataatgaa taatgcacag gaggatttcg tatatgttca
     3541 ataaaattac agcattgtat tgtcgatact cacgagatga cgggcaggag gaagaaaatg
     3601 caagtatcac acatcaacgt aagttgctga aagaatatgc agaaaataac ggatacacta
     3661 atcttcgctg ttatgctgat gacggataca gcggtacaaa tttcaacaga cctgactttc
     3721 agcgtatgct tgaagatatt gaaaacgggt tgatcggaac cgttattgtc aaggatatga
     3781 gccgtttcgg acgaaactat attcttgtcg gtcagtatgt ggaacttgta cttcccatgt
     3841 atgatgtaaa agtaatcggc gtaacagatc actacgattc tacaaaagaa aacaacgatt
     3901 tgtttgcttt tgagagtatt ttcgctgaaa tgtatgctgc cgatatttcc aaaaaagtca
     3961 cagcctacaa acgaaataag gggatgaacg gcggtgttgt caaaacacgt cctgtatatg
     4021 gttacaaaat cgcacctgac acaaaagatg agtggatcat tgacgaaaaa gttgcaggca
     4081 ttgtctacat gatatttgat aaatttgtca atgaggagat gacggaatat caaattgcaa
     4141 actatttgag aaagcataaa gttcttacta catcggcata tgccggttca aagcggtgtg
     4201 atccgacaag aatttatgca tggtcaactt caacgataac aagaatgctt ggcatggcag
     4261 aatatgccgg tgatactgtc aatttcaagt cacgaacggt ttctttcaaa acaagacaga
     4321 tagagagaat tccaaaagac caatggcttg tatttcatga tacgcatgat gcgatcgtac
     4381 cgagagaaat gtttgaaaaa gcacagattc gtctggcaag accgaaaaag ggatttgaca
     4441 agcgaaaata tgagtacaac acttttttcg ccagaaaatg cagatgctct gaatgcggtg
     4501 gaagaatggc gattcaggta gcaaaaggat atgatgggat cgcatataac tgtcagaaaa
     4561 aaatatcctt tcagacttgc aaatcacatt cggttcggga aatgacactt cgcaatatgt
     4621 tcagagatca gatcaccgct ctgcagcaag cactcatttc taagccaaaa gaaatagaag
     4681 aaaaacttgg ggtgtatcag atttcagata tacagaaaga aattgatgtt tccaatcaaa
     4741 gaattgctga gatcaactcc tatatgcagg aactttttga atcgaagatc agaggtgaga
     4801 tcacacaaga tgatttcctg ttactaagca ggcaatatgg caatgagaaa agcaatctgc
     4861 aaagtctggt caatatgctt gctgaaaaat tagcgatcgg aaagaaaaac gcatcaggga
     4921 ttgttgagat actgactttt attcaaaata cagatttttc tgagatcaca gaggaaatat
     4981 gcagcaaact gatagagcaa gtgatcgttg gtgtatacga aaaaaaagga actgtaaact
     5041 atgggaaaca gcctctgaga tttcagatat atgagatcgg atttatagac gagctggtcg
     5101 atgtaagcta caaaacattt cgggagagaa tcgaagctgt acttcttcgc cgatatgccg
     5161 atcacattat aaccaaacgt ccttatgagg tttatgagga acttggactg acctacaaca
     5221 ttatgaaaaa gggactgcaa agagaaaaca ccaacttcaa tacgatcgtc atcgaactca
     5281 gaaaaaaact gatcacagaa tatatcagac aaggaatgtc tgctgatgaa attttcagaa
     5341 tcacaggatt ttccagtgta aatgtcctct atgcattctg ctataacaat tttgaagtgt
     5401 cgtataagca tttaagaaaa gttatccttt cataaaaatc aagggctgtc gcattgtcgg
     5461 cagccttttt gtttccctaa aatcgagtta tcctaaggtt tgtttctcat tggggaaccg
     5521 caaaaatcag ctctcttgaa aaaggttttc acttgtgata gaatatagtc gtcaaagaaa
     5581 agcctgtctg ttacaaacgg aggaatgtga tgtacagaca gaatcctgag tattttatcc
     5641 ccataggaac tgatccggaa cttgcaaaga tgattcgtca ccagcggtat atcaacgccc
     5701 gtgagcatta ttacacggtt ccgtattacg ataatcttct gatttcagaa acagagcctg
     5761 acatacaaca gcttgcttta tggaatgctc ttgcaagaat gaaggtatca gaaccaaagg
     5821 gatacgctct tttaaccaat ttctattttg gcacttatcg ttccgtttca tcatttgcaa
     5881 aagcgaatgg catttcaaga caggcaatgt caagaaaaat caaacgctat cttcgcttcc
     5941 tgcggatcat tgcttatgag gaactggaca gaggtatctt gaaaactgaa tatggagaac
     6001 gcaattttat ctgattgctg caattgagtt acaaacagcg acgattgggc gacaatcagg
     6061 cgacaatttg atgtttatcg ttgccctcag attctttctg tgatataatg aaaacagcaa
     6121 ttttaagcct gtcggtatag gaggaaaata cttatgcaga caggaaaaat cactgcttta
     6181 tacgtcagat tttcttatga tgacggaatt gacgcagaaa gcggcagcat tgaacatcag
     6241 aagtcgttgc tcagaagcta tgccgaatct aacggtttta ccgatctttc ttactacgca
     6301 gatgacggat atacaggcac aaatttcaat cgccccgatt ttcagcgtat gctcggtgac
     6361 attcagaaag gacttgtttc cactgtgatc gtcaaggata tgagccgtct gggaagaaat
     6421 tatctcatgg tcggtcagta tgtagagatg gaatttccaa agcacaacgt tcgctttatt
     6481 gcgatcagtg ataacgtgga ttctgccaag ggaatgaacg atctgctgcc gatccataat
     6541 ctgatgaatg aatggtacag ccgtgatatt tccaaaaagg ttcgtgctat gatacgtcag
     6601 aaaggcaaca gcggtcaggc tattgcatca aagcttcctt atgggtatta caatacaccg
     6661 gaggataagc agaaatggct gatcgatgaa tctgcggcta atgtggttcg caaaatcttt
     6721 gatttgtacc tcaaccaatc ttttgggatg tatcagattg cgaaaattct caaggctgag
     6781 ggtgtaccaa aaccgaccta tcacagacgg ctggcaaaag gcatcaaggt ggaagctgac
     6841 gatctgtgtg tatggagtgc atctgtagta aggggtattc tgcagcatcg ggaatatatc
     6901 ggtgatacgg ttaatttccg aacggaaatc acgtcataca agaataagaa agtcattcac
     6961 aacagcgatg acaagtggag gatctttccg aatacgcacc ctgcgatcat tgacagggaa
     7021 acctttcaga aagtgcagga caagatctca aaagagatca gacacacaag aaatgtacac
     7081 agttatctct ttgcagatta cctttattgc atggattgtc atagcagaat gcacggcagg
     7141 caatgctatt tgaaaggcgg gagaacactc tcagcttatg agtgttccac ttaccgcaaa
     7201 agcaaaggct gctgcttcca tggagtacct gaaatgtatt tgcaagctga ggtgttagcg
     7261 aaaatacaga atgttcttgc ctttgccaaa agcaatcctg ctgagttccg acagaacatc
     7321 cgtaaggcat tggaagagca gtctgacggc agcaaagcag taatacagga agaactggat
     7381 aaggctcaga cccgaacggc tgagatcgac aagtatattc agggcttatt tgaagcaaag
     7441 gtcagaggtg agattgatgg ctcgttattt gcaagtctga aaaaaacgta tgacgaagaa
     7501 aaggaacagc tcaacaggct gataattgaa ctcattacaa agctgagccg tgaaaacgaa
     7561 gcaaatgaaa aagttcagct tctttatgca gcaatcagaa aatatgatgc cgtatgggaa
     7621 ctcactcccg aagttctgac agattttatt gaaaagatcg aagttggtca ggtcagtgca
     7681 gaaaacaaaa agatctcgtt tgaattacga gataatcaga tttctgtttt cttttggggt
     7741 gtcggcatca tcgattttat atgagaaacg gttccctaag ttgggtacga ccagccgaac
     7801 cgaaagctgt ttgatgttat gaccaaaggc tccggcgatg ccagaatgca gcctttgtat
     7861 ttcctgatta ccacagccgg aacggatacc aatagcatct gctatgaggt acatcaaaag
     7921 gcaaaggaca ttctggaagg cagaaagcac gatccgactt tctatccggt catttatgga
     7981 gcagatgaat cggaggactg gactgacccg aaggtttgga aaaaggcaaa tccaagtctg
     8041 gataagacca tcg
//
LOCUS       DAWBGR010000117         8019 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2403_length_8019_cov_4.509920, whole genome shotgun sequence.
ACCESSION   DAWBGR010000117 DAWBGR010000000
VERSION     DAWBGR010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8019)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8019
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2403_length_8019_cov_4.509920"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1932 a         1937 c         2285 g         1865 t
ORIGIN      
        1 atgttatgct ataaggtgta aaggaaagtt tatttttccc ccgaaaggag tttcttatga
       61 aattgcgtta tctgcttctg ctgccggaac tggtactgct gtttttcttt gtatttccga
      121 tctggaagcg aatctgcaat ccgggcaatc tcgccggcat tctggtgtgc ctgtttctgc
      181 tgttcgtgac catatttccg cggaaatttg cccatatcgt gcagcggtgc tgggaaccgc
      241 tgggcggcaa gatcgggctg tccgcagtag ggatcctgct ggcggcaggg ctgacctttt
      301 ccgccgtgat gtctgtgcaa atggtgcgga cgatctgcac gaaaccgact gcaccggaaa
      361 ccgttgtggt gctgggctgc aaagtccgcg gcacccgtcc cagccgtatg ctgatacgcc
      421 ggctggaggc ggcacaggca tatctggagg agaaccccga cgtgctgtgc attaccaccg
      481 gcggacaggg agccggagaa gacgtgccgg agggacaggc catgcgggac tggctgatcg
      541 ggcacggcat tgccgcggaa cgcatcattg cggaggatac gtccaaagat acgcaggaaa
      601 atctggagca tgcggcggca attttgcagg agcaggggct tggagaacag gtcgtcatca
      661 tcacagacgg ctttcaccag tatcgggcat cgctgctggc gaaacgtgcc ggactgcaat
      721 cggaaagcgt gtgtgccaag acgaaccctc tgtatgtgcc tacctattgg gtgcgggagt
      781 ggatggcact gtttcagctt ctggtgctgg ggcatggtta aggaaacact gcataaagcc
      841 tgcatgcaaa gccgtcaggc gggctttacg gttagataca taggcaacaa tgcacaaaca
      901 ggaatggagg gaatctcatg ttacagcaac tgacaccgcg ggtgtaccag ctggattttt
      961 cgcaggcaca agaccgcccc gtgctggggt atattcgggg cgaccggttt tccctgatgg
     1021 tcgatgccgg caattctccg gaacatgtgc aggcatatct tgccgccgtg gaggagtctg
     1081 gttttcgtca gccggatttt gtggcgttga cccactctca ctgggatcac tgcttcgggc
     1141 tggcatcgct gccaatgccg tccattgcct gtgtgcagac aagacagtca ctggaaatgg
     1201 tttcccgcct gcagtggaca ccggacgcat tggcggaaaa tgtgcggaaa ggcattgtcc
     1261 ctcagctgtg tgctccgcgg atccagctgc actttcccga tccggagagc attcgggtgg
     1321 cactgcccac tatggttttt tccgagagca tgacgctgga cttgggaaac tgcacctgtg
     1381 aactgcggca tgtcaccagt gcccacgccc gcgatacggt gatcgtctgg gtgaaagaag
     1441 aacaaatggt atttctgggg gatgcggtgt atcaggagct ggtgggcgat gtctgggtgg
     1501 agcgaccgga aaagctgcga aagctgatac aggaactgga gccgctggac tttcaactgg
     1561 cacagccggc acatcaaaag gcaatgacaa agaccgacct gctggcgtgg ttccggcggc
     1621 ggttggaaag gacggtgcag tcatgacaga tgcggaactg gcgagagcca ttgctgaaaa
     1681 ggcggcggag cagggcggca cagtgtattt cgtcggcggc tgtgtgcgtg accggcttct
     1741 cggactggaa aacaaggaca ttgacattga ggtgcacggc attcccgccg aagcactgga
     1801 acagctgctc tccgaactgg gagcgtgtat gaaaatcggg aaaagcttcg gcatttacgg
     1861 cttgaaagga cacgcgttgg acattgccct gccccgtacc gaacggcgta ccggcgtagg
     1921 acaccgtgac tttgccgtga cttctgatcc gtttctgggc acctatgaag cggcaaaacg
     1981 gcgggacttc accatgaatg ccctgatgca gaacgtgctg accggcgaga tcatcgatca
     2041 tttcggcggc aggcaggact tgcaggacgg cgtactccgg catgtggacg acgtgacatt
     2101 tacagaggat ccgctgcggg tgctgcgggc ggcacagttt gcggcacggt ttcagttttc
     2161 tgtggcggcg gaaacggtgg aactctgccg gcgtatcgac ctgtctgccc tttcgagaga
     2221 gcgcgtgatg ctggaaatgg aaaaggcact gctccgtgcg gaaaagcctt cgctgttttt
     2281 cggagaactg cgaactatgg ggcagctggg acactggttt ccggaggtgc aggcactgat
     2341 cgggctgcca cagaaccaga tacatcacca agagggcgac acgtggacac ataccatgct
     2401 ggtgctggac gaggcggcaa agcggcggga caaggtgcgg tatccgctgg gctttttgct
     2461 ggcggctctg tgccatgact ttggcaaggc agtctgcacc acggaaatcg acggcgtgat
     2521 ccactcctac gggcacgaaa ccgccggact gccgctggta cagcagtttc tggaacgaat
     2581 caccacggaa acaaagctga tacagtatgt gctgaacatg acagaactgc acatgcagcc
     2641 caacattatg gcggctgcca ggtcgcgttt gaaatccacc aataaactgc tggatcgtgc
     2701 ggtggaaccc tttgatctga tacagctaag cgtctgtgac ggtctgggaa agctgccgcc
     2761 ctgcggggaa acagaatcgt tcctgatgca gcggtacgaa cagtttacag ccattatggc
     2821 aagaccatac gtcatgggca aggacctgat tgcggcaggc ttgcagcctg gtgttcagtt
     2881 ttccgagatc ttgtcctatg cccataagct gcggctggca ggtatcgaga aggagaatgc
     2941 tctccggcag agccttgcct acgccagaaa aatgcgaaag gagaatcctg atgcagctac
     3001 atgaaattcg aaaagcactg cacggagaag cgtatgattt cctgcggacc aatccccacc
     3061 tgggcagtcg tgtgatgctg ctggggctgg gcggttctca cgcctacggc acagatacgg
     3121 aaacttctga tctggacatt cggggctgtg ccgcattgag caaggcggag atcctttgcg
     3181 gcgaaagttt tgagcaggtg actgatgttg ccacggatac cacgatctat gcgtttccga
     3241 agctgataca cctgctgaaa gagtgtaatc ccaataccat tgagattctg gggctgaagc
     3301 cggagcacta cctgtatctc tccgaggcgg gaaagctgct gctggacaac cggaagctgt
     3361 ttctgtccca gcgggcagtc aactcgttca gcggttatgc tacagcacag tttcgccgca
     3421 tggacaacaa gtccgcccga atcgccgaac agcctgtaca ggaaatgcac attctgaaca
     3481 gtatccgaaa tgcgaaaaag cattttccgg aacagttttt tcagtatccg gaagatgcca
     3541 ttcgtcttta tatagacgat gcagtcaacc cgcagatgca gaaggaaatt tttatggata
     3601 tttccctgaa gcactacccg ttgcgggact ataaggaaat gtggggcaga atggcggaca
     3661 ttgtgaaaag ctacagcaga gtggggcagg ggcaccgcaa ccaaaatgcc gttacccaca
     3721 acaaattgag caagcatatg atgcacctga tccggctgta tctgatgtgc atcgacattc
     3781 tggaaaaagg agaggtcatc acctatcgtg ctgccgagca tgatttcctg atgcggattc
     3841 gaaacggcga atatctggac gaaaaccagc agcccacagc ggaatttttc gaaatcatcg
     3901 agcagtacaa ggcacgggtg gcatatgccg ccgagcatac cgacctgccg gaaagacctg
     3961 atgaaaagaa gattcgggaa ttggtgctgg cgatacatga aaagatcgtg ctggaggaac
     4021 agtgagaaac ggtttgcagt gttgctgaaa agtttcctgt ctgtcccttt tgcatatttc
     4081 ccgtcccatt ccgcatatca tgaaccaaaa agtttgtggg aggacacggt atgcaatgca
     4141 aatgcacttg gaaaggaatc ggaaaacggg cgggcatcat tctggcagtc agtgctgtca
     4201 gtgtttctgc aatctacttt ggcggcatgc ggatccggca agctgttacc accatgactg
     4261 ccccgaaaaa ggaaacgccg gtgattgttc tggacgcagg gcacggcggt gcagacggcg
     4321 gctgcgtttc cgtcaataat gtgccggaaa agggcatcaa cctgaatatt ctcctgagcc
     4381 tgcgggattt cctgcgtatg gagggctaca ccgtggaggt cacgcgggac acagaccgga
     4441 gcattcacga tgacggcgtt gagggcattg ccaaccagaa aagttcggat atggataacc
     4501 ggctggcact gttcaaccag tacgacaacg ccgtgtgcat ctccgtgcac cagaaccagt
     4561 tcaccgatgc tgcctattcc ggagcccaga tgttttactc cgacaatgtc aagggcagcg
     4621 gtgctctggc acaggctctc cagaatgctt ttgtgaccca gttgcagccg gaaaacacgc
     4681 gggagatcaa gcagtgcggc aaggaactgt atctctgcta tttcagcgaa aatcccactg
     4741 ttatggcgga gtgcggattc ctgtccaatc cggaagaagc ggctctgctg gagtcggagg
     4801 agtatcagca aaaagtggcg ttcaccattt tctccggttt gaaccagtac ctagaagaac
     4861 gcggttgagt ggactgtagg gtgttgttag ccataagtta catcacaaaa aagccgttct
     4921 gtacgcatct tgtacagaac ggcttttgta tttgcaaatt tctccgcggg ttatctccag
     4981 aataccagtg tatctttcag aacgttccag atcgtggggt gtgccttata cagtgccacg
     5041 ccatcgccgg aggtgatgag aatacagaag tatgccagca cggttccggt cattgccagt
     5101 gcgatgactt tctggctgat ttgtgcagca ttggcggaat agggcttcaa cactgccggc
     5161 acttgcagca gcagcatggt ggacatgacg ctgagcacta ccataatatc cagcaggtag
     5221 cgaatggtga tgcctgccat gcagtagtcc acccacgcca gcagaacggc tccgataacg
     5281 gtaatcagca ccagagcacg gcggatccgg cagtcgtccc gctgtttcca ctttttccag
     5341 agcaggggaa tgctgagcac gcccagcagt accgacggca ttgccaacgc tccgaaagat
     5401 ctttcgacaa agagatacat catgcggttg ggaacaagca ctgcgttgtc tttcaggaac
     5461 gggaagtcgt ccatcagttc catggggtgc aggaaatatg ccgcaaaggc agccggaaaa
     5521 cgtgccagac tgatgcggtt tgcggcggtg ttgtctactg tcatctggta aattgccccg
     5581 aattgcagcg ggctgtcaaa ccggatctgg ttgtaccaca tgatcgctgc cgcaccaacc
     5641 agtgtcggaa gcaggaaaca gccggcactg atgagccggt tttgcagccg caggtctttc
     5701 cggaacagca gatgcaggaa aatcggcacc agcagcagga catagaccag caggttggga
     5761 cgggaagccg ccgtgattac cacgccaatg ccgctgactg ccagcaaggc aaagcgtttc
     5821 cacgattttt tcacggaaac cgcggcgaat cccgtccaga gggcgagcag cagtccgaac
     5881 atacccatga gcaggcagag gtaataccgg tcgttgtagt tcagtcccac aaaagcaccg
     5941 cagcccgcca cggcagaaac cagtccgaac aacagcagca acagcggcgg ctgtttgtca
     6001 tatcgccgca ccagtgtcat gatcagtccg aatatggcga tgataatggc tgccacagtg
     6061 atgcagtacg cgaagttcag agtgggaact tttccggtaa tggcatagaa tggcagatag
     6121 atcagcacca ccggagcaca gccgaaatag cagtaatagt gttcgttgta gtaggcatag
     6181 tcccagttat aggaaacgcc atcgcggttg gaattgtcgt agggattttc cagttctgcc
     6241 agttccggtg tcacgtcgaa attcatgtag gaatgcccgt ccgtccatgc ttccagcgtt
     6301 tttccgtaga cgttgctgat gtccacggaa tggatgtcag tgggcttctg gtcgggcaca
     6361 atgaaagcac tgatgcaggc aaggcagccg aacagggtca tcagcaccgc cagattgtgc
     6421 ttccagtttt ttgcctgata gtggatctcc cagacggaga actgcttgca ggcagtcagc
     6481 cccagtgcga tcagcagcac cagcaggaac cgccctgtgg aaaaggcaaa gggaattgcc
     6541 gcataggcgg tgccgctgtg cagagtgatc ggggcatcga tgttttcgaa ctggagttgc
     6601 agcacgtgaa ttttttcata gggctgcatg gcaaattgca gggtgtcata gctgccggtc
     6661 gcctgtgtct gtgcagtctt ttcgaatcgc tgtgtcaggt tgttgtcttt catggagagg
     6721 gaaacctgat acatcttgtc cttgccgtcc gcatgcagcc ggacacagtg cagctcgtcc
     6781 cagtccagat cagaaaaggt cagggtgcag ttgccggtaa tggtgatctc gcccttgtcg
     6841 ttgaaagaag ccgcttcctc atcgctgatt tctgcggtgg aaaggggcag ttcctctttg
     6901 gttttctggg tggtgaagct ggtgatgtgg aacaccagca cttccagcag aaagacgcag
     6961 ccggcaatgc aggcggcttt ttgcagatac ttccgcagaa cagcagtgtt ctgctttccg
     7021 gaaaaggcag ttcctgcacc gagcagcagc agggtgatcg ccagcggcag cggcggaaac
     7081 aggtgggaac cggtgatctg gaacccgcgg atcagagcat gcagaagcag aagcaccaga
     7141 atttctccgg cagtcagtat tcccattctg actgcggttt ttgagaattc agagccagcc
     7201 ggtgccagct tcggcagttt gcacgccgcc agaagcaggg cgttgcatag aattggcagt
     7261 aaaaagtaga gtaacatctg tcaaaatcct ttcataaaaa aatctgtttt tattatatat
     7321 gataaaagga aaatgaatgt gaacaaacat tgcgaaaatc gtaaatataa gatattttcg
     7381 cggattttcg gggcgtgagg tctttttgat atgttgtgtc cgccgataac tttaccttca
     7441 tgcacaaatt gattaagata taactttctg catattccct tagaataatc aatataatac
     7501 gtgcatttat gcgatttaca gtaatttaac caataattca ctgttttttt ttttttttga
     7561 tataattttt cgtggaaatc gaatctccaa caaatttcca aaataaaagc agattgcttt
     7621 tatgtggttt ggggtgaaat gaactatggg aggatttcaa atgtcaagca ccttaaaatt
     7681 atctattttt tcaattgtgg gaattcttgt attctgcatt gtcggaattt ttattccgcc
     7741 agttttactg ctttcggaag cgttttcgat actatgttgt attgtttttt ctatttctgt
     7801 gatttcaaat attgaaacaa acggagcaaa ttttttgtta aaattgtcgc tggcactttt
     7861 gatttctgct gcaatacaat ttgtaatgcg tattatcttt tatattttag gaaagaatga
     7921 cctcttttca ttttgtgtca acagtatctc tgccggtctg gctctccttt cttttacagc
     7981 aacaattttc attatgctgg tgtggctgat attgaaaat
//
LOCUS       DAWBGR010000118         7987 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2410_length_7987_cov_4.725794, whole genome shotgun sequence.
ACCESSION   DAWBGR010000118 DAWBGR010000000
VERSION     DAWBGR010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7987)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2410_length_7987_cov_4.725794"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1903 a         2174 c         1850 g         2060 t
ORIGIN      
        1 ttgcaagcag atggcgtgca aagtcgccag acgctctttg tgcggtattg ccaaagatat
       61 tactgctttt atttcagcaa atgccgcttt tccgcaaaat actgcttggt atccatagca
      121 actacgccgg acagagcaat cagtgcaatg acgttcggga atgccatcag accgttgaaa
      181 atatctgcgg tgtcccatac ggcttctaca gtcagatacg gaccaatgaa cactgccaca
      241 atgtacagcc agcggtaagc cagtgtcagt gtgtggcttg ccttgccgcg aagataggtc
      301 aggcagcgtt cagaataata actccagccc aaaattgtgg taaatgcaaa gaagatcagg
      361 cagagcatca gaatgcagga agaaacctgc gatgggaacg gcagaccgtg ctggaatgca
      421 gcgtctgtga tctgtacgcc ctccagatcc ggattctgcc atgcaccgga aatgacaatg
      481 gacagaccag tcatggtgca gatgataatg gtatcaataa atgtaccagt catggtgacc
      541 agaccctgac gaaccggttc cttggtacgt gctgctgcgg cagcgatcgg ggcagaaccc
      601 agaccggctt cgttggagaa gatacctctt gcaacaccct tctgcattgc cacgatcata
      661 gcacccagtg caccgccggc aaccgcgtcc atgccaaaag cactcttgac gatttccaca
      721 actgcatggg gaatctctgt cacattgcag atcatcataa tcagacagca cagaacatag
      781 gcaactgcca taaacggcac gaccacctgt gaaatctggg agattcgctt cagaccgcca
      841 atgacaacca gtgccacaca aatggtaatc agcaagccca tgatgactgt cgtccatgtg
      901 tagcttgtgt cgaacagggt gaaagcaatg tgcttgctgt cagcatcaaa gaaattgttt
      961 gccgcagagg tgatgccgtt gatctgcgta atggtaccga tgcccagcag accagccagc
     1021 ataccgaaca gtgcgaacag ctttgccagc cacttccagc gggagcccat accattttca
     1081 atatagtaga acggaccgcc cagatagtgc ccttctttgt ctaccgtacg atatttgatt
     1141 gccagaaagc cctcggcata tttggtcgcc ataccgaaca gggctgcaat ctccatccag
     1201 aacagtgcac ccggaccgcc ggccgcgatt gccgtagcaa cgccgacgat attaccggta
     1261 ccgatcgttg cagacagagc cgtacagaga gcaccaaaac tggtgacttc accctcgcct
     1321 tcctcttcat tcgtgaccat gaatttcagt gccagaccga ttcgacgtat ctgcaatcca
     1381 cggatacgaa acgtcagcca gataccgcac aaaagaatca gcaggatcag cggaagcccc
     1441 catacccaag aatcaatgtt tttgatgatc ttcgtcatca tttccatttg cgttcactcc
     1501 tttattattt ttctacagcc aaaaagctgc acattccgac attgtcagaa aaaaacattt
     1561 ttattatatc atactcgcag gggaattgca agaaaaattg tgcaaaactc cagaaaaaac
     1621 tttttttcag agaaaaaaga aagcgttcgg ctttaccaga cagaaaaatc ccgtaaagtc
     1681 gaacgccatt gttctctttt gtatttagga aacactgcat aaagccgtca ggcgggcttt
     1741 atacagtgtt tcttttatcg attgtctacc ttttccggaa acatgtcatg gtgggtcagt
     1801 ctgtcccaag ccacctgttc ccacttcgtc cccggtctgc catagttgca gtacgggtca
     1861 atggaaatgc cgccgcgggg caaaaacttt ccccacactt caatgtacgc cggctccatc
     1921 agcctgatca ggtcgttcat gatgatgttc acacagtcct cgtggaaatc gccgtgattc
     1981 cggaagctga acagatacag cttcaacgac ttgctctcca ccatgatctc ccgcggcacg
     2041 taggaaatgt acagggtcgc aaagtccggc tgtccggtga tcggacaaag gctggtaaac
     2101 tccggacagt tgaacttgac gaaatagtcc cgatccggat gcttgttcgg aaatgtttcc
     2161 agcacctccg gtgcataatc gctggcatac ttcgtattct gattgcccag cagagtaata
     2221 tctcccgttt cttccttgct tcttcctgcc atatcattca cctcccagtg ccggatccgg
     2281 cacaccgttt gctgcaaatg ctgctgcacg gtcgcggcag gtaccgcaga caccgcaggg
     2341 cttttcgccg ccctcgtagc agctccatgt caatgcatag ggcacgccca gttccagtcc
     2401 tttttttacc acgtctgcct ttgtccactt gacaaagggg gcttcgatcc gcagctgttc
     2461 gccgcttccc agataaatcg cccgattcat tgcttcgtta aaggcatcgc tgcaatccgg
     2521 ataggcattg ccggcggcat catcactatg tgctccgtag taaatgactt cgcagccgtt
     2581 ggacagtgcc atgcttgccg cagaagcgag gaacagtccg ttgcggaacg gcacataggt
     2641 ggaaaccgga ctgccgttgg tttcctgtag ctgttccgca taggtttcct tggggatctc
     2701 ctgtgtggaa ccggagagca gagagcagtt ggaatctgca aaaatcacag acaaatccag
     2761 ctgcttccac gcaacgccat aatacgccgc aattttccgt gcagcctcca cttctttctg
     2821 gtgcttctgt ccataggaaa cagacagtgc cagcacttct tctgcaccgt acttgtccac
     2881 tgccatgccg aggcaggtgg tgctgtccac gccgccgctg aaaagaacca ttgctttcat
     2941 ggtcgtacct ccttaaagta attcctgtag actgcggtcc gtctggaacg caccacagaa
     3001 tgtctttgtg gtcgtgcggg aagaaacgtt gcggattccc cgtgccgtca tgcagctgtg
     3061 cgaaccagta atgacaacgc ccacgtccgg cgttcctact gcttccatga ggatctccgc
     3121 aatgtcctgc cccagccgtt cctgcaattg cagccgcttc gatgccatat cgcagatgcg
     3181 tgcaattttg ctaagtccga tcactctgcc gcggggcacg tatgccacat tgacgttcat
     3241 gtcatacatc agtgccatgt ggtgttcgca atagctgaac acgctgatgt ctttcatcac
     3301 caccgccgtc tgagactgac tgggcgaagg ggcttcaaat gttttcccga acatctctgc
     3361 aatttcatgg ttggaatacg aaatgcccgc aaagatctcc tcgtacattc tcgccacacg
     3421 ctgcggcgtt tcccgcagtc cttcacgatc aggatcttcg ccgatcgctt ccaaaatccc
     3481 ccgaatatgg gtttctattg ccttggtatc cattgtcaca cccccttata caccgcgttt
     3541 ctggggatcc cagatcaatt tatgcagctg caattgcagc cgtacccgat tcagtttgtg
     3601 ctcctcaagg aattccacaa tttccaccgg ctcgattgcc ccgaacaccg gactgaaaaa
     3661 gacggtacat tttgccgtca gggcatactg ccggatcact tccagtgcac ggttcaggtc
     3721 gcttctgctg cccaccacga atttcactgc gtcctgactc cgcagcaggt cgaaattctc
     3781 cggcttcatg gcagattcca tgccgctgtc cggcagctta tagtccatgg taaagcaagg
     3841 gcgaaatgcc agtgccgcaa agggttccag tgccacgctg ccgttggttt cgatctccac
     3901 ccgatagccc agggcaccca gtgccgtgat cagttccccg atgtgaggct ggagcagcgg
     3961 ctctccgccg gtcagcgtca cattctgcac accggtctgc cgcacatatt ccaccagttc
     4021 ctccgcagac tcctctgttg cgggagcatc tgccgcattc gcccacttgg tatcacaaaa
     4081 gccgcagttc agattgcagc cgcggaaccg caggaacact gccagttctc cggcatgtgt
     4141 cccctctccg ttgatgctga caaacttctc caccactgcc aaacgattca tgttctcaca
     4201 cctcataaac tgcacaattt tccggcgttt catagactgc cacacggcgt accggcaagc
     4261 cgtctgcggt cagcttttca tagaagcact tggcaaactg ctccgccgtg ggacgaaacg
     4321 gcactgcgat cagccggaat ccctctgcca gaagtgcctc cacagtggca cgctgcaagg
     4381 tgccttcctc ataaatcata gcgtggtcat agctgtccgc cagtgctttc acggcatgct
     4441 tcacatcgcc gaaatcgatc accatgcccc gatagttgcc ttcttcctgt agagtttctt
     4501 gcccgaccgt cacttcgatt ttccagcggt gtccgtgaag attggaacac ttgccctgat
     4561 agccggacag aaaatgtgca ctgtcaaagc tggctgatgt tttgagttcg aacatacggt
     4621 tttcctccca ataaaaaatg cagccgcatt gcacatacga ctgcatccaa aattcatttc
     4681 aaacaaacac aaaacggttt ttgtgtgatt tctagtttcg gatgccctag ttttgttttg
     4741 cgacaggatg gtgcaaacga actgtcgata tgttaccgaa acacagtata ccacactttt
     4801 tttgaaatgt caagtgattt tgtttttaag ggaacactga ataaagcttg tgcgtaagat
     4861 gcatagtcag atttctgggc aatatgacgg aaaatctgca agcagattgc gtgcaaagcc
     4921 gtcaggcggc tttatgcagt gtttttttaa tcttccggtg agatataggt gggagcgttt
     4981 ttcgggctgg cacccttgat gacactatga tagaactgat aggtcatggg aatgccgtcc
     5041 tcccgattca ggtcacagtc tgtgacttct cccagaaaaa tcgtatgtgt gcctgcgtcc
     5101 atcagcttca ccacgcggca ggtgatccac gccattgccc cgtctggcac cggcatgccg
     5161 tccttttccg tacaggaaac gccggcgaat ttgtctgcgt cccgactgga gcgatagccg
     5221 aaaccgccga tcagagccgg atcggtatcc gttgccagaa tcgccagtgc aaacttaccg
     5281 ctttgggcga tctggtcatg tgtcgcgtta tcgtggttga tgcttacggc aatctgtgcc
     5341 ggtgcagagg tgatctgcat cgcactgttt gccacacagc catacggctt tccgttatac
     5401 gatgtcgtca ccagatacac accgtaagaa agcttctgca aagcctttcg gttcatgtcc
     5461 gatccctccg ttattctgca acttccggca aggtcttgat aatggatacc aagaacttca
     5521 gcagtaccac agcatcattg gaatccactt cgttgttggc atcacagtct gcattgctcc
     5581 gctgctgttc agaaagctgt accgtattgg cagccgcctt gttcagcatt accgcatcgg
     5641 taatatccac tctgccgtcc agattgacat caccgtacag gctggcaggt gtaacatcgc
     5701 cgccctttgt agtggtcgtg gtttcgctgc cggaagttgt cgcagtggtt tcgtcagaat
     5761 ccgttgtgac agattcggtt gttgttgttg tggtttcctc ggtagtagaa gtggtaaaca
     5821 cttcagtcac aatcccgatc tctttggtaa gtgtgatcga accgttggtt gcagtcagtg
     5881 tcacagtacc gccgtctttt gcatgcagcg taacattcag accgctgccc tcgacttcca
     5941 gaacgttcgg gtcagaagaa gtccactgga aatcgggatt gctgccggta taggagatcg
     6001 aagcactgaa atcatcgcct gccataatgt agtacgggaa gtctgcccat tctgcctttt
     6061 ctttctctgt tgttactgtg ctttcttcag tagtcgttgt ggtttcttct gtcgtggtag
     6121 tcgtctgttc tgtagtggta gtgcccggct gcggtgtttc gctgccataa ctgaatacaa
     6181 agtcactgtt aatttcagaa gcagatgcaa ttgcactttg tgcagctgtt gtcgaaccac
     6241 cgtaaacggc atacagtccg gcaattgcca gtgcaaagca gccattgtaa tccagtgccg
     6301 gctctgtgca ggaataagaa tttgtgttat cgtcataaga accgttggca tctgtcggac
     6361 caccgaccaa ggtgccgtac aaaatatatt ttgcagtatc cttatccggg tttgctgctc
     6421 tatgatggat atgctgcgga taagaatcgc caaaaccgat cagatagctg cggtttgccg
     6481 gattatttcc cagcaaatag tccatctgcc cttgtgcata ttcattgtat ttgtcctgtc
     6541 cgctggtata ctttgcataa gtcaaagcat acatctgcca tgcacagttg taacgggatg
     6601 caccccagcc gccgccgacc ggataatacg cctgattctt gttgcccacc agctgatcca
     6661 tatcgaacac cagctcgctg ccgtgtgcac tgtacttctg aggatccatg gaatacagca
     6721 gagcagagta accgccccac accttatccc aggaataaac ccagtaatcg tagatgctgc
     6781 cgttatatac gccgttagac ggtgtgtact gtgcctggta agaatccgga agttttccgt
     6841 tgttgttggc aatcgcacac cacagatcac tccacgctac cttatcttca atattgccac
     6901 aggaatagta gctgccgatt ccctcatagg ttgccttcgg atacttttct gtaaactttt
     6961 ccagtgcatt tgcatacttc aggcaagtat ctgcataatc tgcgttttta tcccgaaatg
     7021 ccagagaagt tgctgccaga gctgctgcca tatgaccgga cgcatctacc gacggattgg
     7081 atgcatctgc ccagtatgtc ggtctgttct gtgtttgtac ttccggtgca gtccagatgt
     7141 tgtgatcctg gtcatcgcct accatatagc agaacgcaat gacattaccg ctgtcatcca
     7201 gataagtcac tttcataaag tagtcgcaca tttttttcag aatgtacaga agatgatctt
     7261 cgcttccggt ttctttatag gaatccggat tttcaaaata agaccaagcc agattagtac
     7321 aggaatatcc cattgtattc gagaatttca aatgatctcc tgcatcatga taaccgccgg
     7381 acacatctac ggtatccaag ccgccgtttg cagctttgat tgctgccacg gaactggatg
     7441 acagtccctt tgcactgcta agcgatgctt ccgcatcata agtatgacat tttccacgcc
     7501 aggtcagtac attatcatct gcaccgcact gatttgcgtc aaaaaagtac agagataatt
     7561 tcagtgcatc tccgtatgta ccagctgacg gctgtgccga ggctgtcatt cccgacactg
     7621 ccgaaaccgt tcccgtcacc gccaaaacac cggcggacag tactgccgtc agttttttct
     7681 ttagatttcc tttccagttc atcctttttc actccaattc tttatattcg actcaaaaag
     7741 ccgatcactg attacgtaca tttcattata gcatatccgc tttccattgt caagaaaata
     7801 gaacaaattt catatctttt atgcagtttg acgaactgaa gtgtatttac ggcagcaccg
     7861 cacaaagctt gtacgtaaga tgcgttgtca gatttttcgg cagattgcat agaaattccg
     7921 caggcatact ttcgtatggc aagggatttc tgggcaatat gacggaaaat ctgcacgcag
     7981 attgcgt
//
LOCUS       DAWBGR010000119         7841 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2463_length_7841_cov_3.569741, whole genome shotgun sequence.
ACCESSION   DAWBGR010000119 DAWBGR010000000
VERSION     DAWBGR010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2463_length_7841_cov_3.569741"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1921 a         1862 c         2103 g         1955 t
ORIGIN      
        1 taaaaccttc ttttgttatt ctgtcagata caatgcatcc ttctccattt ggttcattcc
       61 aatcaattaa tttctctatt ttgatactaa gaaaatcgtt gttcttattt ttcttgaaaa
      121 acatgatgat tcatctcctt aaatctcgac ttgtttccct ttttcaagtt ggatacacat
      181 tgtctgccgt tttctgttac gcttccacat tcttctgata gatcagctgc aagcctttca
      241 gcagaagcag gttgtccagt acgatcttgt gccggcagtg gggaatgata aattccgaca
      301 gcccgccggt ggcaagaaat gtaggcgttt cgcccagttc ctcctcaatg cggtccagca
      361 gtccgtcaat gcagccggcg gcaccgtaga gaatgccgct tttcatgcac tctaccgtgt
      421 tgctgccgat gcagcgtttc ggcggatcaa gggaaatttt cggcagctgt gccgtctttt
      481 ccgtcagagc attcatggag atgcccacgc ccggagcgat catgccgccg atgtgccgcc
      541 gctgggcatc gatgacggaa agcgttgtgg cagtgcccat gtcgataatg acaatgggcg
      601 gctggtattc gtgaagtgct gccacggcat cggcaacgcg gtcgctgccc agctgtgccg
      661 gattgtcaat ggcgatctgt aagccggttt tgacacccgg tcccaccaga accacgtggc
      721 agtgtgcaat tttttccacg gctttccgaa ccgcatggtt aatggagggc accacggaag
      781 aaatgatgca gccctcgatt tctgtggtct ggatctgata gatttccaga attgttttga
      841 tagaaatggc aagctccaga gatgtggcgt tctgattggt agaaacccgt tctacaaata
      901 aaatctgctg ctcctgaaag ccgccgatga caacattggt gttgccgatg tcaatggcaa
      961 gtaccatgaa aagacctcct gagatagggc agcgtgtagg ctgccgtaga ttcgggcgtg
     1021 tttccttagg gcaataccgc acaatctttg tgcggtattg cctatgaaat tcataaataa
     1081 ctttactcat gatatatgat atacttcttt cattgctcct gatagattgg aaacacgcga
     1141 tatgttttat tataacacaa tttcggaaaa agtgcaaggt acgttgactt ctctctgcga
     1201 aaatgctata ataaaaacac aaaaattaag gaaacactga ataaagcccg cctgacggct
     1261 ttatgcagtg tttccttaac gcacaaaaac ggaggtgtac agatgcagaa cgttgcagtt
     1321 ttattcgaac aggcggcgga gcagttcgcg gcagcttatc cggaaaacgg acccttgtcc
     1381 ccgtggatca cggaacagct gggcgttaca gaaccgtatc tgcggggaaa agccggcgtt
     1441 gccctgctgc tgcttcggct ggtacagcgg cagcttaccg gcaactcgat tcccctttgt
     1501 cgggacatgc tccccataga agaccgtgcg gcagcagtgc cggagctgga atcagtacaa
     1561 atctggtggc atcgctggct ggggtgtggt atcggcatgg gcttttccgg ctgggaaagt
     1621 ttgtggcagg cagcagacga ggcgttgcag ctgctgccgg aaacactgcc ggcactctgc
     1681 tgtgcagaga ttctgcggag atttgacgga taaaataaaa cccgttcttg cgtgtgctaa
     1741 gaacgggttt ttataggttc atatatagtg ttgttttctt catctgtccc tctagttttt
     1801 tggcacgttt taaggcaata ccacacaaaa cacgcttttc gacaaagcag tcatcgttac
     1861 ggtgctactt tttatttttc tataggaatc atgattccca cccgaaacaa ttctgcactg
     1921 ggctgtacgt ccagtgtgcc cttgtgcttt tccacaaggt gcatggcaga agaaagcccg
     1981 atgccgtggt attcgctgtt gttctttcgg gtgaaaaagc cctgctgttc ctttggtgca
     2041 agcataccgt cgtaggtgtt ttcgatcacc atataccaga atgcaggctt gcaggcagtt
     2101 ttcagccgaa ttcgttttgt tgcttccggc ggcattcgca gacacgcttc tacggcgttt
     2161 tccagcaggt ttcccagcag ggtgcacaat tctacgtcag tcatggaaaa cggttccgga
     2221 atctgaaatt gcagggacag atcaatgtgc tggatctccg ccagattgtc atagtagaac
     2281 agcagagcat ctactgccgg tctgccgcaa taggtttcca tgatttttcc agatccgctg
     2341 ctgtattcgc ccacagagtc cagatactcc agaatcacct gatcgccggt ttgttctgcc
     2401 agcgtgtgca gtgtgcggag atggtggcgg aaatcgtgga caaaccgccg gcggatctcg
     2461 acctgttccc gcagctggaa ggtgtagttg gtctgcatga tcagctgctt ttcggcgttg
     2521 tggttctgct gctggagcag gcggttctgg gtgtaacttt ccgctaaaaa tttccatagc
     2581 atcgtagcca gcatgtagat cagcaccgtg cagacccatt ccagaaacca tccgccaaag
     2641 atcgggtcgt atgccggata gatacgatcc cagaggcagg cacagccgaa aaagagggat
     2701 acatagaaaa gccgatgatt cttttcgctt ttgcgggcgg tcaggaatgc tgtgaagatc
     2761 agatacgccg aaagcagtag cttatatccg gcaagaagtc gggaaacagc ggcaatgccc
     2821 cagtccggta tgctggctgc accgaaaaaa tacaccgaaa cgccaacgca gatccccaca
     2881 gcaacgccgc agctgatgcg ggaagccgtg gcagcaatgc cgcagacctg attgtgcagg
     2941 tagagcagca gagtcagtgt tccgtagcag cacagcgttt ccagccagta agtgggaaac
     3001 accggcagca gaaagagcgt gtgcagcagg ggatacactg tccatccgat catcagcaga
     3061 cagagcagtg ccagaagggc agcgtttttg tggcgcgtcc gaagccagaa gtacccgcag
     3121 atgagaatgg caacctgaaa tgtgaaaatc gtacagagcc gcagccccag ccggattcca
     3181 cgggtgtaat tcaatgcctg cggtgtaccg aacgctggcg gaaaagtcat gccgctgtag
     3241 aaataagact gattattgac agcgatcagt atggtgatca tgccgttttc gtccggaaaa
     3301 aaactgacca gccggctgcc ggtcgcgtcc cgatagtggt ctgggttggg atttccgata
     3361 gacagagccg gatcgccgtt gatatacagc tggtatgctg aaaatacttc cggcataccc
     3421 agagaataac tgatgcgggt actgggaacc tgaatgcgga gcagataagt gccgcagcca
     3481 agttgtgtct ggccgctctg tccggagaga tccatgcggt ttccggactg gagattgaca
     3541 taggtcatat accggtctgg ggcgccgtcc tgaaagtctg ccggtgtcag tagcacattg
     3601 ggataatatg cccagtcatg ccacagataa cggctggggt gctgctccat gtccgcttcg
     3661 gaaaggatca gcagtccgtt gatcggctga gtggtatgct gcgtatattt gttgttgaaa
     3721 cggtacagcc cgctgaaaaa cagcgatgcc agaagcacgg acagcagcat gcccagatat
     3781 aaccggagca tctgcctgcg attctgccgg ttcatcgttt ttgattcctt agccgcagga
     3841 acagaaagat gcccagtcct gccgcagcga ttccacctgc cagccagaaa atccagtacg
     3901 gagcattatg ctctgcctct gctgccggaa tggagaagat tcccagataa ctggtatcca
     3961 tgcggtaggc atggctgtct gcatcgtatt ccaacagctg tgcggctccg ttgacttcca
     4021 gtgccggttc ggcatctttg gaaaagagcg attccggaaa ccacaatgtc acttcttttt
     4081 gcagggaaac attttcgtca tttacggtga cttgtacggc gtagttgccg tcctcttttg
     4141 acgtgatcga aacagtgacg gtgtcggcgt cggtataggg gaatgctccc tgcggaatgg
     4201 aaagggtaac gccgtttttg gagaataccg cattgccgct ttgttcgaac atgcactgca
     4261 gccggtatcc ggaaatggta tcccgatcgc tgtccgagta ttccagcatg ggagccgcag
     4321 cagttgtctt gctctgggcg gtgctggttt cggaagtggc agaagcagct gtggtctggg
     4381 cagtggcagt ttccgcaggg gtgcggttga cagaatatgc ctttttcttg tgttcttccc
     4441 gcagcgaacc ggcgttgtca ttttcctctg ctgcggcagg cacagctgcc ggtgccttga
     4501 tctgagccgc ctgtgtttgt gtggtggtgg aagcggcaga tcgtgtttct gtcggtgcag
     4561 cttccggagc cgcaggagcc ggctgggaaa ccgccggcgt gggagaagcc acattgtcgc
     4621 tgccgtcccg atcgccgccc atggagatca ccttggtctg actgccgtct tcgaaaatgc
     4681 cgatgtactg cgaacgggta tcttcggtct gcacataaaa ataggtgtac cacttttccg
     4741 gcagacgatt tcgatggatg aaaaagccgt tctggcaaat cgatgccatt tcgttgtcgc
     4801 acaatgtcca tgtacagtcg tccagctcag catcgtcaca gggctgctcg ctggaatagg
     4861 cataaacgct gtcgccctca tggagctccg gcaggcagta aatgacgtgg ctctccgctg
     4921 tggtgcgtgc acagaacagg tcaaatgctt ccggctggct gatgcagacc ggaatggaaa
     4981 tgttctccaa ctccggagca agcacatagt ttgcagcatc tctgtcggaa tcgtcaatgc
     5041 acagatgcac agtaacggta tagcttcccg cagtctgaat gtccaccgct tcccagccgg
     5101 agatctccgc cagaagcagg gcttcgccgc cgttctcatt ataagcgatc agataggaaa
     5161 ccgcatcttc cagataatac tgagattctt ccagttcgag agtatcccct tcggcaatga
     5221 gaaagccgct gaaaaacacg tcggcagaag cgatcgcggt gagaacctcc ggttcttcct
     5281 ccgcttcagc tgaaaccgga atgctgctgc cgaggcaggt cagcagaaaa aggaaaagca
     5341 cggcaaattt gccgaaacgt tttgaagcac acatcaaagc atcaatcctc ctttgccatt
     5401 tggtcgtaga gataggtacg ccagagttcg ccgatttttt tcttgttgcg gatattgatc
     5461 ggcagcgttt cgccgttgga gagccggaac agattgccgt ccatgtcctg aatgtgctgc
     5521 atgtttacga gaatgccgcg gatacagggg agaaagcgtt cgtcagcctc cagcgatgcc
     5581 gtcagctttc cgaaggaaac agcaagagag atgcattccg tttccgtgtg gatcaccgtc
     5641 gtgtgtccct gtacatcaat ataaaggatc ttttcggtgg gaatgctgcg ggggacgttt
     5701 tcctgtacaa tttccagaaa cggcacagca taatccggct gtagttccag aaactgcatt
     5761 gcctgttcga attcctccgg cacaatgggc tttaccagat agtacactgc ccgcacggaa
     5821 tagctttgcc ggagaaagtc ggtgctgctg gagagaaaga tcagttttgt gcggctgccg
     5881 gcggcgaaca gttccctggc agcgtccatg ccggtctttt gcttcatata gatgtccaga
     5941 aaaaccacat cgaaagtccg gtgctgtgcg gcttccagat attcttcagc acaggtgaat
     6001 tcttccagtt ccgcggtgat gcggtgtttt ttcaggtaga cgtgcagata taatttcaga
     6061 ctgttacggt ctgcggcaga gtcatcgaca agtgcaatat tcatatgcaa aaatcacctc
     6121 cgttgcattg tagatttttg aataggcata cgaatacaga ttacattata gcatattttt
     6181 catggaatgt ctagggattt ttttgcgttt catgaagaaa aactgcgttt tgcgtaaatc
     6241 gtattgcata attgtgaaaa atccagtata attatatcat caacagcggt gcttttcgaa
     6301 agcagtgctg gaatagaaat cagagcagga cagatgaaag cgtgttctgc tggggctgaa
     6361 aaggagttag aattaatgaa actgagaaaa atgttagcat ggctgactgc gatggcaatg
     6421 ctcggaactg ccgcactggc aaccggctgc ggctcggaag aaacatcttc ctccaaggcg
     6481 gaaacctctg ctgcggaaac ggaagaagct gctgatgcag cagatgatgc agaagtagat
     6541 gaacttgcag aagatgatgc agaaagcggc agtgtggacg atgaaaccgc agcgtatgcc
     6601 gatgcactgg caggcactct ctggctgggc atggatacag aatataactg ctatgctatg
     6661 ggcttcaatg aagaagagat cgtatttgaa gcagatgacg gttcttctgc ttccggttat
     6721 tggggtgtta ccgcaggtga ccctaccatt tatattttcg atgatgcaga actgaccaac
     6781 cagatcgctt ccattccgtg gagctatgat atggaaaatg acgtgatgat cctcaacaac
     6841 aatgtcatca tggcacaggc agataactac agcttttctg atgcagcaac tgcaatgcag
     6901 gaaatggcaa ctgccgcaaa ggtacaggaa tacttgcagg gtacttactg ggtaggcacc
     6961 gatgacacca gtgcatctgc tatttctatg agcagcgaca cgctggagat cattgaactt
     7021 agtcaggacg gcacactgga tcagggctcg ttcctgtgga gcatggatta tgatactctg
     7081 aatgcctacg atgagaatta caatcttctg gcaagctttg gctgggatat ttcggaagat
     7141 ggctcggaac tgcagctgac cagcgacggc gagagtatca tatatacaca ggtatcggaa
     7201 gaagatgcca atgccgtggt agaatatctg tattcggcaa tgggtctgga agaatccgca
     7261 gaataagtgt gtaaaggaaa cactgtataa aggcagcact gccgcacagg ctttgtgcgg
     7321 tgttgcctga agtttgtctg atggtttggc atgcaatctg ccgaaaaatc tgacgacgca
     7381 tcttatgcac aagttttgta cagtgttgcc ctaaaactat ataattataa tactgttcca
     7441 atggcagact ggaacgacat gggacgaaag gagattatga ccatgaaaag caaaactaca
     7501 gcaattattc tctcgattct tctgggagaa ctgggcgtgg atcgttttta tctgggatat
     7561 accggtttgg gtatcctgaa gctgatcaca gcaggcggtt tcggaatctg gtggcttatt
     7621 gatctgattc tgatcattac cggaaaactg acagccaagg acggcagcga actggtataa
     7681 gcacaaggca acaaaggcat tgttcccctg tgcctttcaa ctgttcatat tggaatgata
     7741 caggctcaca aagccgctgt aaaaagcaga gatttttatc tctgcttttt ttgaaaggca
     7801 ataccgcaca aagagcgtct ggcgactttg cacgccatct g
//
LOCUS       DAWBGR010000120         7789 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2483_length_7789_cov_4.183476, whole genome shotgun sequence.
ACCESSION   DAWBGR010000120 DAWBGR010000000
VERSION     DAWBGR010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7789)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2483_length_7789_cov_4.183476"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7363..7372
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1693 a         2117 c         1880 g         2089 t
ORIGIN      
        1 cgggctttat gcagtgcttc catagggtaa caataaaata aaacgaccgc aagccggaaa
       61 gccgcggtcg tctggggatt ttctgtggtg aatggagtcc attttttgaa atggtctact
      121 cattccgata atcgtccaga aagctatgca ccacgtgatc tttctggtac gaaatgattt
      181 tgttcaggtt gatattctcc acgctgcgga aaatgttctt ttcgatctgc ggattttcct
      241 ctgccagctt ggcgatcagc tgcttcacca ccatacgctt ggaaaccgtc cgttcatcag
      301 gatcacaggt agtgcaggtg tccgtgaagt ggcaggcaca ctggataaac tgcaagtcgt
      361 gataccggca ccagtgcttg atgtcggctt cccgaatcag gtacatgggg cgaatcagct
      421 gcatgccctc gaaattggca ctgtgcagct tcggcatcat ggtttccacc tgtccgccgt
      481 agatcatgct catgagaatc gtttcaatga catcgtcaaa atggtgtccc agagcgatct
      541 tattacagcc cagttccttt gccttgttat acaaatgtcc acgccgcata cgggcacaga
      601 gatagcaggg gttattctgc acgtgataca ccgaatcaaa aatattggtt tcgaacacgg
      661 taatgggaat ttgcagcagc ttggcgttgg attcgatgat ctgccggttc tcggcactat
      721 agccgggatc catgaccaga aagaccagtt caaatggaaa ttttccgtgc cgctgaatct
      781 cctgaaacag cttcgccatg agcatcgagt ccttgccgcc ggaaatacac acggcaatac
      841 ggtcgttggg ctggatcagg tcataggtct tgatcgcctt ggcaaaccgg ctgaagatca
      901 cccgatgaaa ctttttccgc agtccctgtt ccacttcagc agccttttcg tttttggttt
      961 cccgccggat cttctcctga agccacttga catagccacc caccagattc actgccgcgt
     1021 agccttcttc ccgcagctgt tccgccacat cagcactcag gataccgctg cggcagtaga
     1081 tcaccagtgt tttatccttg ggaagattct cgtgagaggc ttccagttcc ttttgcggga
     1141 tcagcactgc cccgtcgata tgccccagag caaacgccgt gctgtcccgc atgtcgatga
     1201 tctgacaggc agacggctcc atggctgcca gttcctgtat tgtgatttct tctatcatgt
     1261 acttacctca ccaaaccgcg gtgatacgcg gcgtttaatt ctgaaatttc tttttctccg
     1321 tcgatatacg gctggaaata gctgtccacc agaccgccgc ccagattgtc acagccgttg
     1381 cagaattcac aggtctgtcc gcagacctgc agcgactggt tcacaatacg gattcgttct
     1441 tcccgtgtgg tatctttgat caatatggat tgcatgatga ttcctcccat agaaaaacct
     1501 gctttcagtg gcattccacg aaaacaggtt ctcagacagc acatgtgcag atactttagg
     1561 aaacactgca taaagcccgc ctgacggctt tgcacgcaat ctgcttgcag attttccgca
     1621 caagctttat tcattgtttc ctttacgctt cgctgtccag tttttcaatg gcttcccgca
     1681 gggtacgcca gcccagcacg gcacacttta cccgtgccgg catatgggaa atatcctgta
     1741 gtgcagaagc ttcttccagc tcctccactt ccgcatcagt tgccgtgccc ttgatcattt
     1801 tcaggaacag gtctgacaga tgcagtgcct ctgccttggt cttgccgatg atcatgccga
     1861 tcataatatc cgcagatgcc tgcgaaatgg cacagccgtc cccgttgaaa gatgcgtcct
     1921 gaatcacgcc gtccttcatg accagattca gccagatgtc atcgccgcag ttggggttga
     1981 caccttccag ctgaatggtc ggacattcca gatcatattt gaattccgga cggacgttgt
     2041 gttccgtcag gatctcgtta taaaaattat tcattgccaa agcccatcct tctccgaata
     2101 ttggaaacgc tctctaagaa ccggtcgatc tcctctttgg tgttatagaa catcaggctt
     2161 gcacgggcag tggaatggat tcccagatga tcgtgcagcg gctgtgcaca gtggtgaccg
     2221 gcacgaatat ccatgccgtc gctgctgagc acttcgctga tgtcgtgcgg gtgcacctga
     2281 tcgacggtaa aggaaacgat accggtatgg ttgtcctccc gctgtgaccc cagcacatgt
     2341 acatggggaa tggcattcat gccgcggaac agatatgctg tcagttcctg ctccacagca
     2401 tggagagtgt ccataccgta ggattccaga taatcgattg ccgcagccag tcccacggct
     2461 ccgccggcat tgaccgtgcc tgcttcgaat ttgtggggca gttccgcata gactgcatcg
     2521 tagcgggaaa cggtttcgat catttccccg ccggtgagga acggcggcat tttttccagc
     2581 agttcctgct tgccgtacag cacgccgatc cccatgggag ccagcatctt atgaccggaa
     2641 aatgccacaa aatccacgtc cagtttctgc acgtcaatgg gcatatgagg ggcactctgt
     2701 gccgcgtcca tcaggattac cgcaccgcag gctctggcac gcttcaccag ttccggaata
     2761 tcgttgacac agcccagcac gttggaaacc tgtgtcactg ccaccagcct ggtgtgttcc
     2821 gaaaaagcct cgtccatttt ttccttgaga atggtgccgt ccggctcgca ttctagaaag
     2881 accagcttgg caccggtggc acgggaaacc atctgccacg gcagaatatt gctgtggtgt
     2941 tccagaatgg aaaccagaat ctcgtcaccg gctttcaggt ttgccatgcc atagctgtac
     3001 gccaccagat tcatgctctc tgtagcattt cgggtgaaga tgatctcctc aggacaggca
     3061 gcatgaataa accgctgcac ggtctttctg gcgttttcat agcgttccgt ggcttctacg
     3121 ctcaaggggt acagtccgcg gagcacattg gcgttatact tttcatagaa ttcctgcacc
     3181 gcatcgatca cacacttcgg cttctgggaa gtcgccgcat tgtcaaaata gacccgatcg
     3241 gtgttctgga gcagcacaaa gtcctgccgc acgttttccg gattccacgc tgtcacgtca
     3301 gatcacctcc tcgattttct ggtgcatata ctctgctgct ttctcgtcag cagtttcctg
     3361 acaaagcttt tccaaccgag cctttgccac gatcttttcc gcttcgctct tgctgatacc
     3421 gcgggcttca aagtagaaca gcatgccctg ttccagttcg ccgatagaag caccgtggct
     3481 gcccttgaca tcttcttcgg tgcagaggat cagcggaatg gtcttattga caacgtcatc
     3541 tcccagcagg agcacgtttt cttcttcctt accctccgag ccggcacagc cacgcttcag
     3601 gtcaatggtg ccgcggaatg ccttttcggc tccgtcgtac aaggtgccgt ctgcctgaat
     3661 gtcactctgg gtgttcttgc cgatatggtt ggcaatcacg ttcatatcca gctttttgga
     3721 cggctttacc agatagccgg atttgcaggt gaagctgctg ccgtttcctg ccagatcggt
     3781 acggataccg gaaatcacat tgccctgtgc cagatacagc cggatcacct cgaaagaagc
     3841 cttttcgccg gtgacacagc ccacatcatt gatgcactct gcactgccat cggcaaggat
     3901 ctgcaccagc cgaacagttg ctccggcttc tgcacgaatc acagtccgca gtgccgcatg
     3961 ggaagcgtct gctttccaga tctgcaccag tgtcagttcg ctgccggctt ctgcctgtac
     4021 atggacaaat gccgcacagt cgccgccgtc ctcgctgccg atctccatac gcagggtgtc
     4081 gctgccgttt tccggaacgg tcacggtcat gccccactga aacaggttgt ccacattcct
     4141 gcccacgccg gtttccacgg cactacagtc ggtgcagggc tccagtgcgg aaactgctgc
     4201 aacaccgttt tcggaaacgg tctgcacggt gcagggcttc accggtgtat agggttctgt
     4261 tttgcaggca ttgacatgca gtctgtgcca tgtcagtgtc ggaagtgcat ttactcgaat
     4321 tgtattattt tccatcatgc tcacctcacc caatgctgcc tttcatttcc agacgaatca
     4381 gattgttcat ttcgatcgca tattccagcg gcagttcctt ggaaacgttg tctgcaaagc
     4441 cgctgacgat gcatgcccgg gcatcttctt cgctcagacc tctggacatc aggtagaaaa
     4501 ttgcttcgtt gctgatcttg ccgatctgtg cttcgtgtcc cacgtctgcc ttgcgggtgc
     4561 ggacatcgat cgccggaatg gtatccgact gggactgtgc gtccagcatc agggattcgc
     4621 aggaaaccga ggacttcgag ccctctgcgt ccttattgac cacaacggaa ctgcggaacg
     4681 tgctgatacc gccgtccttg gaaatcgact tggtgttgat ataagaactg gtgtccggag
     4741 ccgcatgaac gaccttggca ccggtgtcca gattctgtcc ctttccggcg aaagtaatgc
     4801 cggtgaactc catttttgcc cccttgccct tgaggatact catggggtac agataggaca
     4861 catgagaacc gaacgaaccg gaaacccatt ccaccttgcc gccctcttcg accagtgcac
     4921 gcttggtatt caggttgtac atgttcttcg accagttttc aatggtggag taccgcagtg
     4981 tggcattctt gcccacgaac agttcgacac agccggcgtg gagatttgcc acattgtatt
     5041 tcggggcgga gcagccctcg ataaagtgca ggtatgctcc ctcttccacg atgatcagcg
     5101 tatgttcaaa ctgtcctgct cccggtgcat tcagacggaa ataggattgc agcggaatct
     5161 ccacggacac gcccttcggc acgtacacaa aggaaccgcc ggaccagact gcaccgtgca
     5221 gagccgcaaa cttatggtct gtcggcggca ccagcttcat gaagtgcttg cggatcatgt
     5281 ccgcatattc gccgtgcatg gcactctcca gatcggtata cactacgccc agatcggcaa
     5341 cttcctgccg cacattgtgg tacaccagtt cggagtcata ctgtgctccc acgccggcaa
     5401 gggactttcg ctctgcctgc ggaatgccca gacgttcgaa cgtattcttg atgtcctccg
     5461 gcacgtcgga ccacttggtt ttcatattgg tattggggcg gacataggtg acaatgttgt
     5521 ccatgttcag tccgtcgata gacggccccc agtccggcac ctgcaattcg ttgtagatct
     5581 ggagggagtg cagacggaac tgctgcatcc agaccggatc gttcttttct ttggagatct
     5641 gttctaccag tgccggtgtc aagccggcat cgatcttata ggcatcggtt tctgtatctt
     5701 taatatcgta gacacttctg tcaatatctg cgatctgctg tttttctttc acgcgttttc
     5761 accctttcgg tattcctgca acacttgaga gctcgtgttc ataggcgtct atctacgtct
     5821 tttcggcaaa ttttgttgca tactgcgtta aaaattcttg ccatacgaaa gtatgcctgc
     5881 aaatttttgc cttgtctaca aaaaaatttg ctcgaaaatc cgcatatatt tcctacgaac
     5941 acaagctctg agaaacgctg cataaagttc gcctgacggc tctgcatacg atctgcgtac
     6001 agtcttcttt atccagtgtg attactctgc attttcataa gcggcaaagc cgttttcgtt
     6061 gacttcttca atcagggaac tgtcggaggt cttgacgatc ttaccgttca ccatgatgtg
     6121 cgtatagtct accggcagtg cttccagaat cttggtgctg tgggtaatga ccagcaggct
     6181 gcccctggac tgcttgcggt atgcttcgat gcccttggaa acggtacgga cagcatcaac
     6241 gtccagaccg gagtcggttt cgtccagaat gacaaattcc ggattcagca tcagcatctg
     6301 aaggatctca gtctttttct tttcgccgcc ggagaagccc acgttcaggt cacgctctgc
     6361 ataggacgga tccatctgga gcagtgccat tttttcttcc agttcctttt tgaagctgaa
     6421 cagacggaca cgcttgccgg tcttggcttc cagtgcactg cggataaacg cactgacggt
     6481 gatgcccgga atctcaatgg ggttctggaa cgacaggaaa atgccgttct tggcacgttc
     6541 gttatccggc atttcgttga tattctgtcc cttatagatg atgtcgccgc cggtgacggt
     6601 gtaagcagga ttgcccatga gtgcagcacc cagtgtggac ttaccggtgc cgttcggtcc
     6661 catcaggacg tgtacttcat tctcgtgaat gtccagatcg atgccgtgaa gaatttcttt
     6721 ttctccggca catactttca aattctgaat acgcagcaga tcattatgac tcatgttaca
     6781 tcttctcctt catctttata atttaccgca tcggcaaggg taatggaatt cagatagtta
     6841 ttgatcagtg cagaaagccc tttccagatc ggcagtgtgg aacaggaatc cgcagtttcg
     6901 cagatcggcg ttccgttttc aatgcacttt accggttcga tggattcttc cactgcccgc
     6961 agaatttctc ccacgctgta ttgctccggc ggacgggaca ggcgatagcc gccgcccttt
     7021 ccgcttgccg cttccacaaa ctttttcttg gagagcatcg aggtaatgtt ttccagatat
     7081 ttgagagaga tattttgctg ggcagagatg actttcagcg gtgtatacgc ttggtcgtcc
     7141 acctgtgcca gacacatcat cagcttcagg gcatttcgcc cttttgtgga cacaatcata
     7201 gttaccactt tcctttgctc tgtttgattt cattatacac ctactaacct agtatgtcaa
     7261 tgggacaggc ggcaggttta caaaaacttc atggagaatt ttcatgatgc tatgcggaaa
     7321 attcttggag attttgactg gaaacgacat tgcatgcagt gtnnnnnnnn nnttccctaa
     7381 tccatgcaaa acctctccgc cagcttcggc ggtttctccc tgttgccaat ttttctttcg
     7441 ttagctttgg caggttttat gccatttata ccataactgc accgaatgcg gcggcaacgg
     7501 catatgtccg gcagtttatg cccgtggtcg gggcatgaaa aaagcacctc gggtgagatg
     7561 ctttgaaaat atttgtatta ctcccagaat tgaaatccgc tgtcgatcaa tcgtaaaata
     7621 gaattgttgt caccagagta tttttcaaca ccgcataaat gcaaagctgc ggtggcaaat
     7681 tctccgatag tatacgcaac agacctcaaa tagcggttat cgtttttgga taattgaatc
     7741 tggttaataa ttgactcgtc attgactttc catttaacag tgcatataa
//
LOCUS       DAWBGR010000121         7779 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2485_length_7779_cov_3.598912, whole genome shotgun sequence.
ACCESSION   DAWBGR010000121 DAWBGR010000000
VERSION     DAWBGR010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7779)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2485_length_7779_cov_3.598912"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7033..7042
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7457..7466
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1969 a         1976 c         2063 g         1751 t
ORIGIN      
        1 gcaagcagat ggcgtgcaaa gtcgccagac gctctttgtg cggtattgcc tttataatct
       61 aacggcaaaa aggcactgtg cagaagcata gtgccttttg tgttgccttg acaaaagcaa
      121 ttgccacgtg ctataatgcc tttatcagga ggaatgtgtt atgagagaaa tctacccgtc
      181 ccattatgcg gcgtttcact gcattgccgg agcctgtccg gacagctgct gcaaggaatg
      241 ggaaatcgtt gtggacacgg catctgcgga gagataccgt tctgtttccg gcacgctggg
      301 agaacggctg cggcaggcaa tgattaccga tgcagacggc gacatcgttt ttcaggaaca
      361 caacagtcgc tgtccttttt ggaatgcaca gcagctttgc gatattcacg gggaattggg
      421 agaagatgcc ctgtgcgaaa cttgccggct gttcccccgc atcacgcagg actacgggga
      481 ttttatcgag tttgacatct cccccgcctg tccggaagcg gcaaggctgc tgttttcgat
      541 gacaccgaag cagtggcagc tgacagaacg cagttctccg gcaataacag aggaaccgcc
      601 ggaatatgca gcggcgaaca tgcggcggtt acaagaacaa cgacaagcat tattttttcg
      661 gttgcaggac gattctgctt ctgcctgtgc acagctgtcc gcctgtctga cacttgcgga
      721 acaatgggca ggtgtttcgc aggaaccggc ggctgttccc tacatggaag aagctgttct
      781 gtcctttttg cgatcctgtg aagtgctgac ttcggactgg aagcagatgc tggaacaggc
      841 tgcaaatgca ccggaaccgg aagatctttt ggcatacacg cctgcactgg atcaagaaat
      901 tcggctgttt acaatggata tgctgtaccg gtattatctc cgtgcggcat atgccgaaac
      961 tccagaagca gaaattttgc cgttgcaaat ggcagcgttt gctgtgtgcg tggtgctcct
     1021 atacagcaga cggctggaat acagcagacg gctggaattt cacaccgcag aacagcggca
     1081 gcgaatctgg cagcttttcg ttaaggaaat cgaatatgac gggaacaatc tggaagaact
     1141 ggaatggcag ctggaaacca acgctgcttt ttctccggcg gcgttccgtg catatttcgc
     1201 ttcgctggga ggtgtccaat gatttctctg ttttcccacg ccatttctgc actgctgtcg
     1261 aagctgtttg acaaatggac aaacaaatat atcgacagag aaaacgaaga aaattcttat
     1321 cggaaatgcg tgagagtttc taaagctttc agtattgccg gaggcatctg gtttatctgt
     1381 tttgcggcgt ttggcgttct cagcattgtt atctgggctg acggcatgac ggattgggat
     1441 tctttcgctt gcggtgtcct gtttctgctc agcagccttt cgggtatttt ctgcatgctg
     1501 ttcagacgta attaccgcat cttttacaca gatacatttg tggaaaagca cagtaatttc
     1561 gggaaaaagc aggtctaccg ctgggcagaa ctgatttcgg ttcaacaaaa aggtgctgtc
     1621 tacacattga cttttcccag cggaaaactc cgcattgttt ccagcaagga gtatgttggc
     1681 agtgataagc ttgtcgattt tttggaaaaa gaaatatttt tttgaaaaaa gggcttgaca
     1741 agtctttcgt tctatgatat aataagaaac tgtggcggct atgccgcaaa aaatcagaga
     1801 ttcacaggac tactgtcggc acaatggccg atgggctgct gttgatacaa acaacagata
     1861 caggaggtga aaccccatga gagaaggcat tcatccgaat tttgaaaaga ccaccatcac
     1921 ctgccactgc ggcaacgtaa tggaaaccat gtccaccaag aaggacatca aggtcgaaat
     1981 ctgctcgaag tgccacccgt tcttcaccgg caagcagaag ctggttgaca caggcggacg
     2041 cgtagatcgt ttcaagaagc gtttcaatct ggactaattc agattacatt tttcgaacac
     2101 gacaatggaa aagggacggt attttgttta ccgtcctttt ttgtctacgt ggaggtagat
     2161 acaagaatgg aatatcgtac cattgccgca ccggtatcgg cttcgtttgt ggagaaaaaa
     2221 tcggaattta ttgcccagct ttttccggta cgcacgcagg aagatgctgt ggaagccatt
     2281 gaaaatgtcc gcaaacagca ccgcagagcg aggcacaatg tctacgctta tctgctgcgg
     2341 gaaggcaacg cttcccgcta ttctgacgac ggcgaaccgc agggcactgc cggcatgccg
     2401 attctggacg tgctgcaaaa aaacggactg acggatattt gctgtgtggt cacccgatat
     2461 ttcggcggcg tgctgctggg agcgaacggg ttggtgcggg catactctca cagcactgcc
     2521 ctcgccattg aaaatgcaca ggtcaaagtc atgctcccct gctatccggt ttccattcag
     2581 acggactatg ccctgtacgg gaaaatcgcg taccacctgc cgcaggaaga tattctccag
     2641 ctggacacga tctttgaaga tcaggtagag ctgaaactgc tggtgcggga taccctgtgg
     2701 gaacccctga aaaaggaact gatcgacctg accagcggac aggtggagat cgtttccggc
     2761 gggctgcaat acgcagactt ttccaaggcg aaaaaataat tttcgaaaaa agaggagttt
     2821 tgcaatttcc tgcgtatata taaaggaagc attgaggcgg cacggcactg acttgcctgt
     2881 ttcgccttga ataacccctg cccttttcgg aggtgatgtg ccatgaccat tcgtgaacaa
     2941 ctggaacaac aggaactgga atacctttca ccggacgcat gcccaaacat cggacacgcc
     3001 cgccggcttc gtccggaacc gccctgtccc tatcggacgg agttccaacg ggaccgcgat
     3061 cgtatcctgc actgtaactc gttccggcgg ctcaagcgga aaacacaggt tttcctttcc
     3121 cctgccggag atcactaccg cactcgtctt acccatacgc tggaagtcgc acaagttgcc
     3181 cgtaccattt cccgtgctct gcggctcaac gaggatttga ccgaagccat tgcactgggg
     3241 cacgatctgg gacattcgcc tttcgggcac agcggagaat ctattctcaa cagcatctgc
     3301 ccccacgggt accggcacta tgagcagagc cttcgtgtag ttgacctgat tgagaagcac
     3361 gggcagggac tgaatctgac ggcagaagtc cgcaacggca ttctctgcca taccaatcgg
     3421 gtggcagata ccaaagaggg caacgttgtc cgtgtggcag accgcattgc ctacatgaac
     3481 cacgacattg aggacgcgat tcgtgccggc attttgcaaa aagaagaact tcccaaagat
     3541 gtattggaag tattgggtga caccaagacc aaacgcatca cctgtctggt gggagccgtg
     3601 gtggagcacg gttcggaaac cattggcatg gctccggaga ttcaggcaat gcacgacaag
     3661 ctgcatgctt tcctgtttga acgggtatac tataatccca tcgccaagca ggaggaaaac
     3721 aaggcaaagc tgctgctgga aacgctgtat acctacttta tttcccacgc ttccctgatg
     3781 ccgccggaat atctggcaat tgcagaaaag gaagatgtgc accgtgcggt atgcgactat
     3841 atttccggca tgagcgatgg ctatgccgtg gatctgtatc aggaattatt cattccgaaa
     3901 agttggaaat catgatcttg ttccaaaaag gcggtgacaa acaatgacat acagcaatga
     3961 ctttttggaa cagattcggg cagcatgccc catggaaacg attgccggca actacgtcaa
     4021 tctgatccgg cggggcagac actatgtttg caactgtccg tttcactctg aaaaatcacc
     4081 gtcctgtacc atttttccgg aaacccagag cttctactgc ttcggctgcg gggctggcgg
     4141 cgatgtcatt acgtgggttc gccgcatgga caatctggaa tttaccgatg cagtcaagca
     4201 gctggcggaa cgatgcggtc tgcaagtgcc taccgatcgg gaggcagacc gccgtgcata
     4261 tctccgcacc cgcatgttcg cagtcaatcg ggaaactgca aatttctatt tccggaatct
     4321 catttccggc agcgacaaac gcgggctgca atatttcatt tcccgccagc tgaaaccgga
     4381 aacgataaaa aaatacggac tgggctacgc ttcagacagc tggaatgccc tgaccgacta
     4441 cctgctgcaa aaggggttca cagaggaaga actgcttctg gcaaatgtgg cacaccgttc
     4501 taacaaagga aatctgtatg atgcctttcg cggacgtgtg atgttcccca ttgtggatac
     4561 gcggggagct gtgatcggct tcggcggcag agtgctggac gacagccagc caaagtatct
     4621 gaacacggca aaaacaccgg tgttcgacaa gggcagcaat ctgttctccc tcaatttcgc
     4681 caaggacagt tcttccacga tcatgattct ggcggaagga tatatggacg tgattgcgat
     4741 caaccaagcc ggcttttcca atgtggtggc gacgctggga acggcgatca cgccggaaca
     4801 cgcccgaaag ctctccagat ttgccaagga agtggtcatc gcctacgaca gcgacggccc
     4861 cggacagcag gcaacccaga aggcgatcaa ccggctcagt gaggtgggaa ttgccacccg
     4921 cattctccac atgaccggtg caaaagatcc ggacgaatac atcaaaaaat acggctcaga
     4981 gcgtttccgg ctgctgctgg aaagcgctgg tgatgccatc aatttccggc tggaccggtg
     5041 caaagaggga ttagaccccg acacggaagc cggaaaagta cagcttctga aacgggtggt
     5101 atctgtactg gcggaaattc agaacccgct ggaacgggag gtatacctct cccgcacggc
     5161 gaataagtgg gagatctccg cagaagttct gcatcagcag gtggaacgca ccatacgcag
     5221 caaaaagaag ttggcttcta cgcaggcgtg gaaagatatt attgaacaca ccgtccgacc
     5281 ggaaccgcag cagccgcagt ctgcaattcc gctgcgggag tacaaggcgg aggaacgcat
     5341 tctttgctac gtgctgaacc gaccggacga gtcggcatgg atcatagaac agatccagcc
     5401 ggacgcgttc cctacaccgc tgtaccggca ggtgcttgag gcatttgctg acagcgtaaa
     5461 gcggcaaacc ggcttttccc tttcagcaat gggcgatgtg ctctccgaca gtgaaatggg
     5521 aaagctcagc ggcatttctg cccgcaatca ggagatccct gtgacagcag aagaagtcaa
     5581 ggactgcatt cgtactctga aaaacgatat gccgtctgca atcgaaactg atgaagactt
     5641 attaaaactg attcagaaaa agcagcaccc tgacgggtaa acaggaggaa caatactatg
     5701 gaacagaaaa agatgactat tgatgcactg atggaacagg gcaaagcaac cggaaaactg
     5761 accaccaagg aaatcaccga tgtattggac gaactggact tcgacaccga tcagctcgcc
     5821 tccattatca gcagctgtga acagctgaat attgagattg tagacgactc tgttgctgat
     5881 gacgtgttga acgacaatga tctcaacatg tccgacgatc tggagatgac cctttctaca
     5941 gagggcatcg ccattgacga ccctgttaaa atttatctga aagaaatcgg ccgtgtgccg
     6001 ctgctctctc cggacgaaga aaccgagttg gcaaagcgta tggcagaggg cgacagctat
     6061 gcgaaaaagc ggctgtccga agcaaacctg cgtctggttg tcagcatcgc caagaagtat
     6121 gtgggcagag gtatgcagtt tctggatctg attcaggagg gcaatctggg tctgctgaag
     6181 gcggtagaaa agttcgacta caccaagggc tttaagttct ccacctatgc gacatggtgg
     6241 atccggcagg cgatcacccg tgccattgcc gatcaggcaa gaaccattcg tattccggta
     6301 cacatggtgg aaaccattac caaggtcaaa aaggtttcca gccagctgct gcacgaaacc
     6361 ggtcacgatc cgtctgcgga agaaatcgca gacaagctgg aaatgccggc agaacgtgta
     6421 cgggagatca tgcgaattgc acaggatccg gtttctctgg aaaccccgat tggtgaggaa
     6481 gaagacagcc atctgggcga ctttattccg gacgacgacg ctcctgctcc ggcagatgcc
     6541 gcttctctga tgctgctgaa agagcagttg aatgaagtgc tctccacgct gacagaccgt
     6601 gaggcgaaag ttctgcggct gcgtttcggt ctggaggacg gtcgttcccg cacactggaa
     6661 gaagtgggca aggaattcga tgtcacccgc gagcgtattc gccagatcga agcaaaggct
     6721 ctgcggaaac tgcgtcaccc cagccgcagc aagaaggtca aggatttctt ggactaaagg
     6781 aaacactgca taaagcctgc ctgacggctt tacgcagtgc tcacttaagg caataccgca
     6841 caaagattgt gcggtattgc cttaatgtaa gtataattcc tgctgcacac cgatctcgcc
     6901 ttgcagcgta taaataactg tatatgtaac gccgtcctcg gtttcgagga cggttgtttt
     6961 tttctccaga atctccgtct gtgcaagaaa attgtcctca tatcgctgaa tctgctcctc
     7021 cacattttgt gcnnnnnnnn nncggcttct gccggtgtca aggtcagcag acgggtgcgg
     7081 tattcgtgaa aactctcctg atagattccc agcggcaatt ccctgccgaa cagagaaaac
     7141 cagtgatagc cggaagtccg gatactgtct gcatagggcg aatctgtact gcccagcggc
     7201 agatgccatg tgaacaagtc cagatacttc cgcgtggtca ccgcaccggt gggacggcgg
     7261 agttcttggg tgtagggaca ggagaattgc tccgtctgtt catactgccc gataatgctg
     7321 cccatggcat gacgaattcc cacgtgtccg gtttcgtctg tgtacagccc actgaccagc
     7381 aagtcccctt tccgcaccgg ttcccctacc agcttcatca gctgtccccg ataaatcgac
     7441 accgaaacaa tatgccnnnn nnnnnnatga tgttggacgg cttgcgggtc atctgcattt
     7501 ccggttccgg tgtccgttcc agaatttcca ctaccagccg atttcctgtg tgccgcatgc
     7561 ccacccatgc cgcttcctct acggaagtgc agagcctgtt ttcacagtgc ccgaaatcga
     7621 tcgacggaat ccagctgccc cgctgtacgc cgcattcttc cagaattgct gtgacctccg
     7681 atgtggaaat ggtgtcgtcg gcaatcacct cgatctgcat gacgatgttg gaaccgtaga
     7741 acagcagtcc cgctgcaagc agaattccca gcggcaccc
//
LOCUS       DAWBGR010000122         7774 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2489_length_7774_cov_3.255085, whole genome shotgun sequence.
ACCESSION   DAWBGR010000122 DAWBGR010000000
VERSION     DAWBGR010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7774)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2489_length_7774_cov_3.255085"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4029..4038
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2121 a         2009 c         1865 g         1769 t
ORIGIN      
        1 aaaaatttga cgacgcatct gtcgcacaat ctttgtgcgg tattgccttt acttttgatt
       61 gttgacagtg acaaaacgca tgccccagtc agaagacttg aggtctgctt ctttctgata
      121 ggtttcctgt aactcattat agcgttgatt ttgcagggca tttgtggcag tcatttccca
      181 gccagcttgt tctgcggctt ctacataata gaccaggcag gaaccataag agctgtccag
      241 aatcgtcaca tcaccggatt tccgtgcggc atcaaagcac cagttctgcc atgcagagga
      301 aagctgtccc tctgtgatgt tctggtacag tccgccggaa acggtggtat cttctgtgta
      361 ctggtctgcc agtgctgcaa aagcggactc ggtctgttcc ccgctgttcc attcctccag
      421 tgccttttct gcggcactgt gagcaccgtc agaggattca taggtgtttg tggtgaacag
      481 gatctggcga attgtgacag ggtgagattc gtcccgcttc ggttcgtcca acagcaggca
      541 cacggtgatt cgtttgttat ccgtatctgt aaaaagattg gagtcccctg cctttgcttc
      601 gccggaaaat gcccactcgc tgacatcatc accctctgta taagagatgt tatgactgta
      661 gagtgtgctt aattgggact gcaagtcctc ctctgtcaaa gacgtattct tggcatagta
      721 gtcatacacc catgactgga attcatcttt cgtattgcac cgccgcagac tacgggcaag
      781 ttcttccgct tcgctccggc tgatcgttgt actgtcgccg gaatcatcat aggaaaaaga
      841 gaattccata taactgcaaa cgtccatcgt cttggcattc tctttgtaat aggattccag
      901 ttcatcatcg gtaaaagaca tgttctcctc cacgctggtg ctgaaactct gagccttgaa
      961 atacagttcc agtgcatggg tgacgttgtc ctgtgtcacg ctgctcggca gtgtgctgaa
     1021 gtccatctgt tcctttgcag ttttcaccat agcggagata ttctccggaa attcgtaatt
     1081 ttccttgtct gccgcttcac agtacgccag tacgttttcc gcgtattttt gtgccgcatc
     1141 ggtgaaatag gcttcccaag tggtatcgtt atcgtagttc tggtcagcca gcggcttttc
     1201 tgtatccggc aggttcgccg aatcagaacc ggtgctgctg ttgatatagc tgttgtaaat
     1261 ctgccatgcg tagaacgata cttctgccgc attgacagaa taatgttctg tcttttccac
     1321 cggaatcatc tgggtcatcg gtttttcccg cacaaaccat gtgattccgc cggcaactac
     1381 ggcgacagct gccgcacagc ataccacggt ggtaacaatt tttctcttgt ggtgtttttt
     1441 cttttcttcc tttgccgttt ttcggcgttg ttctctccgc tgctgtgcca gaaattcctg
     1501 cttttcggac ggtttctttt tatttgccat aatattcctc gtattctgct gcacgactgt
     1561 catgcaggca atgtgcttgt attcttagag ccagttcagt aagagatact gaactggctc
     1621 ttatagtata gcatatcttt tacaaaagtg caagggcaac actgcacaaa ctatttcgaa
     1681 ttattcttat ccatgcggtt gtacaagaag ctgatcccaa aacagatcac gccgcaaatc
     1741 aggaaactga atgcccgcag ttccagcttg ccgtaggaaa tgtccagcag gatcagctta
     1801 cagatgctga ccagcaccaa tatcaagcca tagatgcgaa ccggtttgcg ggatttccag
     1861 aagccgaaca gaatcagcag aaccgccagc accagcaagc caacgctcag cagcaccttc
     1921 ggcaaagaga acccgcagaa caataccaac agccatgcag tcagctttcc gcagaacagc
     1981 atttccctgc ccgtcatacc ggaacggatt tcgttctttt tcagcagttc catggagccg
     2041 gcaaaataca gcagtgtcaa tgtcagagcc agtgcgattc gctccggcag atgcagtgtt
     2101 tcacaaaaca gcatcagcaa gctgccgatt cccagaagtg cccagttccc cacataggaa
     2161 atccagtagc ggacatttct cgtttcccgg tacaattggt acagcagcac cacgccattg
     2221 accatgccga gcagagaaag aatccagcct gcggcacgct gcggcgacag gtctgcaaag
     2281 cagggcagat tccggagaat ccagccggta cagagcagga acaagccata ggcagcattt
     2341 ccgtaaaaag gcatacgctg ctttttccca aacaggaaca gcactgccag aaatgcgaac
     2401 cacaggacgt gctgcaatag gacagccggc actgcggctc cggaaccgca ggttcgtaca
     2461 aataccggaa gtgccgggat ttccagcaag agcaacgccg aaacagcagc ggtgagataa
     2521 aactgtgtct gatacaaaaa tgttggcatt ctccgccgct gtactgcgaa ccaaaagccc
     2581 ataagcaata cggctccgac tgcatagggc agactggaca gcagtacagt cccaacagtt
     2641 atatcaaacg aaatggttgg tgttccaaat tggtacacgc caaagcacag caggatcagc
     2701 aattccagaa atgtcgaaaa atacaaaatt gcctgcactt tatctgatgc acattgattc
     2761 cggcggaacg ggcgagacag caatgcgaac tgcaccagaa ctgacaggag cagcattccc
     2821 caccgcacat aatcccacgg agcagcaact gtgccattgc ctgcaacggg atattttgtc
     2881 aaccgcccca aaatttcaag cagcggcagc agcgtcagga tatgagcaat tattgctgtc
     2941 accgcatagc ggatctgcgt tttttgcacc gaccatgcca acaaagcgaa tcccagcaga
     3001 atatatgcgg tcaggcaaac gccgtccgcc tccgacaaag cagtccccag cgtaaccgaa
     3061 aggaacagcc ctgccgcggt caacggctgg aacacgtctg gtcgcggttt tgtaaacaag
     3121 ctgcacagac cgacccagat cagaagcaga atgcacacgg tcagcatact gattttcgga
     3181 aacaattgct tgcacaggag aatccactgc aaaagaactg ccatgccgga aacgcccagc
     3241 acggtgaaga atcggcggca gtacttccgg tgcatgcccc accagccaag cccacagaga
     3301 agcagcccaa tggtcagaaa aacaagcacg attcccccga ctggcagaaa ttgccgctgc
     3361 aatatccgca atccctgcaa caatgtgagc actgccggaa tcggcagaag ccatcggaaa
     3421 atcccaaaaa aagcacttcc ggtttttaac gggaatcggt gatgacaaaa cagtacgcca
     3481 taatatgcca tactgcacgt gagcagcaat acggcttgtt tttcccaaat ggtcccggaa
     3541 gaaaaaattg ccagcgttac gaaaaacagc agtccgatgc cgctgtagcg tatgccaaca
     3601 ttggcgaaac ggctgcccag aatgacacag cccaatgcta ctgcacagat acagccgtaa
     3661 aggaccgccc ccgtggcaga atacagaatt gtcagggcac atgtcagaag cagatatttt
     3721 ccgcagaaca atacacccat gccgtttgat ttccagcggg gttcccgaaa atagcggtcg
     3781 ctgttgacag agaaaactac aaccaatgcc aatgtgagca gaagtttcca cggcaggaaa
     3841 agcttctcca atataagcgg cacgaacata aacaatattg aaaaaatggc gagcagattg
     3901 acaatttgcg tgatgccaga caatgccctg ccattttcca aacggcggca cagtactatc
     3961 gtaacacagt tgcaaagaga gaaaaagcta tacagcatca caaagttctg ccaaaacgag
     4021 ccattcagnn nnnnnnnnga tgaagggcaa ctacaccatt cagcacagaa agcagcggaa
     4081 tccatgaagc cagcaaaaac agtccatagg aaaactgcat ggcaaagggg aacttcgccc
     4141 ttctgccgaa tccagtcagc aggagcaggt acagacacca gctcacgatt cgcagcacac
     4201 cgccgcagaa cagaatgcat gccgccgcgg tcagtgctgc gattccaaac gtcagacgat
     4261 aagtggcagt atgtacgcgg tctttctgtg ccgcatacca gaagcctatg cagaacactg
     4321 caccaagcag caatgcggac tgttccagcc agtctgggaa cgacagcatg ctctgccaga
     4381 atccaagcat gcacagccac aacaacagct gcgtcggaac acagcggaaa ctccagacgg
     4441 aatcagaagc tttttgccgt tccagccagc ggtgcatatc ccagaaatac tggaacagca
     4501 gaaagccagc cagaagcaga ctgcacactg ccatggaaat tgcctttgtc tgtacatgca
     4561 ttacgccgtt ccgaaaaaac gtatagaact ggtctgcctg aatcaagatg cgaatgccaa
     4621 gcagtcccac acaaatcccg tttgcatata gcgtaaacaa gtgcttttcc ggtcgttcct
     4681 gccggttggt cagcagatag aacaatgcac tgatgcagac gtaacacgtc attatcagca
     4741 gttgaaaatt tgcctgttcc gaaaaatggt ctgaaaatga tgagagctga aacttggtgt
     4801 aattgtccag ttgggaaaat actgctacca ccatgccgat atgaccaata ttctggaaaa
     4861 ccggcagctt cccgcgggag agcaggtaca tcagtcctgc ccacagcaaa aacaaggcgt
     4921 acatgcccag cacggagagc agttcataat accagtagcc cagcataatg cccagataca
     4981 ggattcccgc accgcagccg gaaacggaca gaaaaaacgt ccggtatgcc gacttcctgc
     5041 gggaagccag catgccgagc agcatcagaa acaagccggc tgccagaatg ccgcacagct
     5101 gtatccaacg ggaacccgat gcccatgccg tcagcaaaat gccaatgaac accagtaagg
     5161 aagcaaggat tcccagcaag ttcttgccga aattgaattc cttgtccttt tttggcggtg
     5221 gtacaggcgg ctgcccgttc tgcggcgttt taggcggttc ttgtctatcc atgataatcc
     5281 tccaaatgtt tgtaatcaat tgttggtatc tttattatag atgataggcc gaaaaatgtc
     5341 aagagatcat ttctttttct tgttattttt tccagttatt ctgtagatta agcaacagga
     5401 aatcggggaa agatatggat acagcaagga ggtcgtgtta tggcatttca aaaagtgatg
     5461 atttccggta tcaataccgc tgacctgaca gtactgaaag aagaagaaaa aacggcactg
     5521 ctgcgggaaa ttcgggatac cggcagcaag gcagccaagg acaagctgat tcagggcaat
     5581 ctgcggctgg tgctgagcgt gattcagaga ttcaccggtc ggggagaaga tattgacgat
     5641 ctatttcaga tcggtgtcgt tgggctgatc aaagcgatca acaacttcga tctttccaaa
     5701 gaagtccgct tctccaccta ttgtgtgcct atgatcagcg gcgaactgcg gcggtatctg
     5761 cgggacttca accagatcaa ggtcagccgt tccctccgtg atctggctta tcaggcaatg
     5821 caggcaaagg aacggctgac gaccaatctc cagaaagagc cgactgtcag cgagatcgcg
     5881 gcggagatcg gggcaaagcg ggaagatgtg gtggtggcac tggagagtgt cagcgaaccg
     5941 gtttccattt atgagccggt atattccgaa tccggagatg cgttgtatgt gctggatcaa
     6001 atcagtgaac gggatactat ggaaaactgg ctgggagaac tttctctgcg aaatgccata
     6061 cgacagctgg gaacacggga acagaatatt ttatacctcc gcttctttca ggggaaaacc
     6121 cagacagaag tggcaaagga aatcggcatt tcacaggcac aggtttcccg actggaaaaa
     6181 aatgcgattt tacaggtgcg ggacgctgcc aatctgcatc accattccta gggcaatact
     6241 gtatgttagg agaggatccg cttgggcata tcccacaaaa ttccttgcca gacacactat
     6301 ctgcctgcgg aatttttatg caatctgccg aaaaatctga cgatgcatct tacgcacaag
     6361 ttttgtttgg tgttgccctt gctttttttt atcgattatg gtataataaa tgcaaggcaa
     6421 taccgcacaa agagcgtctg gcgactttgc acgccatctg cttgcaaatt ctgatgcgga
     6481 ggcgtttctt tcacgttttc acatctctca cgcgaatgga acaaagaacc acttatataa
     6541 tagtatagcg tgctcacata aactgtcctg cttttatgtg aacacgcttt cgtacaatct
     6601 gctcaacgga gggaaatcac ttgaaaattc aaaaatctgc agaagattat ctggaaacca
     6661 ttctgatctt atacaaccgc cgcggcacag tacatgccat tgacatcgcc aacgaactgg
     6721 cattttccaa gcccagtgtc agcgttgcca tgaaaaatct gcgggaaaac ggatacatct
     6781 gcgtcaatga aaacaacgag atcacactga cggaatccgg cagaaaaatt gccgaaacga
     6841 tctatgaacg gcacacgctg ctttctaact ggctggtggc tctcggggta gatccgaaaa
     6901 cagctgcgga agatgcctgc cgcatggaac atgtcatcag ttcagaaagc ttcgctgcga
     6961 tcaagcagca tgtacaggac aacaacgaaa ttcccgcaaa agctgaaatg taaggagcga
     7021 aaaccatgct gaaacgaatc gttaccattc aggacgtttc ctgctttgga aaatgttcct
     7081 ccaccgttgc cctgcctttg atctctgcta tgggcgtgga aactgccgtg atccctaccg
     7141 ccgttctgtc aacacacacc ggcggattca ccggatatac ttttcgggat ctgacggcgg
     7201 acattccgca gatcgcggca cactggaaaa cattacagct gcatttcgac ggcatttaca
     7261 ccggctatct ggggtccaga gaacaggtac agatcatggc agactttttc gatgcgttcc
     7321 gcagcgaaga tacggtcatt gtggttgatc ctgtcatggg cgatgccggc agcatgtacg
     7381 ccggcttcac accggatttt gcagtggaaa tgcgaaaact ctgcggaaaa gccgatgtta
     7441 tcgccccgaa catgacagaa gtttccctgc tgctgggcat tccctatgcg gaacagtgtc
     7501 cgctggaaac tgtgaaaaca tatctgaaac agttgacaga gatcggctgt cccatcgccg
     7561 tcattaccgg tatccgaaac ggcgatcagc acggtgccat tgcctacgac agtaccaccg
     7621 gcagctacta cgccgcctat cgggaacatg ttccagagcc aatgcatggc accggtgata
     7681 tttttgccag cgtactctcc ggtgctctgg tccgcggggc atcgatcgaa caggctcttt
     7741 ccattgcggt caactttaca gccgacacca ttca
//
LOCUS       DAWBGR010000123         7708 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2506_length_7708_cov_5.627205, whole genome shotgun sequence.
ACCESSION   DAWBGR010000123 DAWBGR010000000
VERSION     DAWBGR010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7708)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2506_length_7708_cov_5.627205"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1753 a         2256 c         2139 g         1560 t
ORIGIN      
        1 caacaccgca caaagcccgc ctgacggctt tgcacgcaat ctgcgtgcag atttttgagg
       61 aggtatttcc atgctgaaac cgatttcccc tgacagttcg attttttaca tcggcgtaga
      121 tgacaccacg attgatttgt ttgaaaacca gtaccccgtt ccccacggcg tttcctacaa
      181 tgcctacgcg attcgggacg aaaagacggc tctgctggac accgtagacc cccgtgccac
      241 ggagcagtgg ctggaacagg cggcggaagt gctggacggc acggctccgg attatctggt
      301 gatctcccac atggaaccgg atcacgccgg cagcattcag gctctggcgg aacgctatcc
      361 ggaaatgaca ctggtaggct ccgcaaaggc tctcgctatg ctgccccagt tcttccggaa
      421 tctcccccgg aaccccaccc ttgccgtcaa agaaggggac gtgctggaac tgggcaccca
      481 caccctgcac tttgtcatgg caccaatggt gcactggccg gaagtcatgg tttcctacga
      541 gtctgccgaa aaggtgctgt tctccgcgga cgcattcggc aagttcgggg cactgtgcac
      601 ggacgaaccg tggacagacg aagcacggcg gtatttcctc aacattgtgg gcaagtacgg
      661 cacaccggta cagaccttac tgaaaaaggc ggcaaaactg gacatacaga ccatttgccc
      721 gctgcacggt ccggtgctga ccggcgacct cgcaccctat ctgcacctgt accagacgtg
      781 gagcagctac acgccggagg aaaagggcgt actggtggca cacgcttcca ttcacggcaa
      841 cactgccaaa gcggcggcgt atctggcaga acagctgaag gctctgggcg aaaaggtcac
      901 agtcatcgac ctgtgccggt gcgacatggc ggaagcggtg gcacaggcgt tccggtatga
      961 ccgcatggtg ctggctgcgt ccagctatga cggcggcgtg ttcctgccga tggaagaatt
     1021 cctgctccac ctgcaatata aggcattcca gaaccgcacc gtcgcactgg tggaaaacgg
     1081 ttcatgggca ccctgtgccg caaagaacat gcgggcagtg ctggacacca tgaagcagat
     1141 cactgtactg gagccgaccg tcaccattcg ctccgccgtc aagccggagg acgaggaaca
     1201 cctgcgggca ctggcagaag cggtgcggca ggaagcgtga tgtgcttctt tgggtgaaag
     1261 caggtcaagt tcgctcgtcc tcgccgccta aggcaatgca ctgcacaata acagtcaccc
     1321 cgcaaaacac aaaaactgca cagagatccc aacaaaaacg gatctctgtg cagttttttc
     1381 ctattgtacc aaaatcattt tcgcatcgca ctttcgcagg gcgaccacgc cgccgcgaac
     1441 cgcgtatgcg gtaggatctc cgccaccggc ggtgtgcaga cagcggacgg ctgtgccggt
     1501 gatccagccc agatcctgta accgctgccg gatcgccccg tgcagataca tactggtaat
     1561 caccgccgtt tcgccgtatg ccagcgtgtg cagcggtcgg acaggttttt ctgaaggcat
     1621 tgctttctcc tcctgttcct atcctatgca gacacaccgc ggaatgttac ggcaataccg
     1681 ccagtgcttc ctgataatac gcccgcagct gcatcattct gtccttgtcg gaaaatgccg
     1741 ttgctgcggc acggttgtgg gcacccattt gccggcggag cagcttattc tctgccagct
     1801 gtgcaatggc atctgcacag cttgtaacca gatcgccttt caggcaaaag gcactctcac
     1861 ccttttcggc gtagaccttt gtaccgtggt tccggacggt gatcagcggc agaccggctg
     1921 ccatggcttc cagtgccgcc atgccgcagc cctcccgacg ggacggcata cagaaaatat
     1981 ccgccgcccc cagcaggtcg ggaatatccg tgcggtagcc cagcaggtgc acccgttctt
     2041 ccagatgcag tttttccgtc agcttctgca aaaacaccat atctggcccc tcgccgcaga
     2101 tgacgtagtg cagctgttcc tgtgtccgca gccgtgtcat tgcctgcatc accacccgca
     2161 gccgcttctg catgcgaagt gttcccacag acagcagcac ggtggcttcc tgcggaatgt
     2221 gcagttcccg caggatgtcc tctctggtgc gggcaggggt ggcgtacttg tcgcagtcca
     2281 gccccacgtc cggcagttcg gtcacctgct ccagattcag gtgctttctg gcataggcgg
     2341 catcttccgg acagacggcg aaaaagcggc tggtctgttt ccggtatttt cgctccaccg
     2401 ccgagaacca cagccgttct gccagcaggc agccgtggaa aatgcgaaaa tcgtgggcag
     2461 tatagaacac cggtatttgc agttcctgtg cggcacggcg accataccag ccggcaatgg
     2521 tgccgcggca gtgcagcaga tcgggctgga gttctttcag aagcggcacc agcttttcat
     2581 aggcggcatg gttccggcga acggctcccg cactgtctga aaagggcagg tcatgccagt
     2641 gcagctgggg gaacatctgg ttgtactcct gcagggctgc ctgtgagatg ttgccttcct
     2701 gcatgttgca gaccatgtgg atctcacagc ccagcgtctg gagggcagtg aggtcatatt
     2761 ttgtaaactg caccagcgtt gccgccgccg cagcgagaaa aaccacacgt ttcatgaagt
     2821 aaagtccttt cgtttccttt gagaatattc ctattatagc acaattttat agaaaagtct
     2881 atagggaaca cgaaaatcaa ttttcatgat agcctcccct gaaaggggag gggaaccgcc
     2941 gttaggcggt ggaggggttt ttcgtaaaat ctttttatga catttttcaa tatgaggaaa
     3001 agtttaacac ataaaaaaga tttcaccaaa acctctccac cagcttcgct ggtccccctc
     3061 tcctttcagg agaggcttat ttttctgcaa cataaatttt agctgttgca caacaatagc
     3121 agctacgccg ccgccaaaaa caaaaaacgc cccggggtga gggacgtttt ttgctatgat
     3181 atagggatta ttggggattt gtccacttcg catcggggtg tacgcgaagt ggttcagagg
     3241 atgttacatg ctctctttga gcatggtttt agtatacacg cccaatgtgt tcgtttcgtg
     3301 aaaccatgct gaaaaaccgc tgaaaaccac aaggacgttt tttcacacaa aatagacttc
     3361 gtgtcgattt ccagaacttt cctcaatttt tgtggatatt agacagattc cgcctgcccg
     3421 ttcacagcaa accgccaaaa atttcgcctg caccggcgtt ctgcccccca aattgcaatt
     3481 ttcctcttgc tatttgtttc gaaatgtgtt acaataaaag tgtcaaaaat caagcggatt
     3541 cgaacacgct ttttgtgtat tcagacgaaa aagcatggga aagtgccctt gctttcatgg
     3601 aacaatagga ggtaatacga cacatgagta ttccggaact gttacagcag ctgacgttgg
     3661 aagaaaaggc aagcctttgt tccggcggcg acttttggca taccaaggcc atcgaacggc
     3721 tggggatcgc cgccatgatg atgagcgacg gcccgcacgg tctgcgaaag caggacgaaa
     3781 ccggcgacca catgggcatc aacgacagca tcaaggcggt ctgcttccct gcgggctgtg
     3841 ccactgcgtc ctcttttgac cgcggtctgg tggaacgcat gggcaaggct ctgggcaacg
     3901 agtgtcaggc ggaaaatgtc ggcgttctgc tggggccggc agtcaacatc aagcgttcgc
     3961 ctctgtgcgg cagaaacttc gagtattact cggaagatcc ctacgttgcc ggcgaaatgg
     4021 cagcatcgtt cattcagggc gtacagagcc agcaggtggg caccagcatc aagcactttt
     4081 atgccaacaa tcaggaacac cgccgcatga gcagctcctc cgagatgaac gcacgcacgg
     4141 cacgggaaat ttacctcgcc gcatttgaac gtcccattcg caaggcgaag ccgtggacgg
     4201 tgatgtgtgc ctataaccgc atcaacgggg tctatgccgc cgaaaacaaa gaagccctca
     4261 cggacattct gcggggcgaa tggaatttcc acggctttgt ggtcagcgac tggggtgccg
     4321 tcaacgaccg cgtggctgac ctggaatccg gcatggatct ggaaatgccc tcctctttcg
     4381 gcatgcggga tgcagaaatc gtcaaggcgg ttcgctgcgg cagactgccg gaagccgtac
     4441 tggacagagc cgtcacccgt atcctgcgaa ttgtccagcg gtatctggac ggacggaatc
     4501 ctgatgcagt attcgaccgc gatgcagacc acgaactggc acgggaaatc gcgtcccagt
     4561 gcatggtgct gctgaaaaac gaagaccata tcctgccgct ttcgaaaacc gaaaccgctg
     4621 cctttatcgg caagtttgcg gaaagccccc gttttcaggg cggcggcagt tctcatatca
     4681 actgtcacaa ggtggagtct gcactggaaa tggcggcaga ctaccccgtc ctctacgcca
     4741 agggctatga aacctcggac gatgtggtgc gggaagacct gatggcagag gcagtggctg
     4801 cggcaaaggc ggcaaaagtc gccgtggtat ttgccggcct gccggacacc tttgagtccg
     4861 agggctacga tcgggagcac atgcggctgc cggcctgcca gaatgccctg atcgaagccg
     4921 tggcagaggc aaaccccaac acagtcgttg tcctgcacgg cggctcgccg gtggaaatgc
     4981 cgtggctgca tcaggtcaag ggtgtactgg aagcatacct ctgcgggcag gcagtgggcg
     5041 gtgccacggt gcagatcctg tacggcgatg tcaaccccag cggacatctg gcggaaacgt
     5101 tccccataaa gctggaggac aacccgtcct atgtgggata ccagggcgag ggtgacgtga
     5161 ccgaatatcg ggaaggcgtt ttcgtgggat accggtacta caccaccaag aaaatgccgg
     5221 tgctgttccc ctttgggttc gggctgagct atactacctt tgccgtttcc aatctgcgga
     5281 cggcacagga cgagtaccag tcccatgatg tcatcaccgt ttctgtggac gtgaccaaca
     5341 ccggcgaccg tgccggaaaa gaagtggtac agctgtatgt actgccccag accaagaccg
     5401 actcggtaca gcgtccggtg caggagctgc gggacttcga aaaggtggaa ctggctcccc
     5461 acgaaaccaa aacggtgaca ttcaccctcc acgccaacag tgcgtttgcc tactactgta
     5521 cccagaaaaa acgctgggta gtagaaaccg gcaagtatct gctggcagcg ggcaactcct
     5581 ccgaccacct gactacagaa aaaatgctta ccattcacgg ggaaaaagca ctccccgcgg
     5641 agatcacact gaacacgacc ataggcgacg tgctgcggat tccccatgcg gacaaggaac
     5701 tggaaaaata cgccggtgca ttcggcattt ccagcggttc ttccgatgaa aacgccatgg
     5761 gcgaaagcac cgcagacatg atggaagcca tgatgaaata tatgccgatc cgcggtctgc
     5821 tctcttttgg cggcggtgag gtttctattg ccgagtgcaa ggcactcgtg gaaaagctga
     5881 acgtcctatt ggaaaacgca aatttgaaca ataggagcga atgacatctt ctccaccttt
     5941 catttgtcag acggcacata tcggggcaac gaccggtatg tgccgtttgc tatggcatcg
     6001 caccacagac ggcaccgcag attgcagaac accgtcaccg acagtaccgc tttcggcagc
     6061 acccacggaa tcgtcaccag cggcagcatt tgcagggcat accaccccag cagtgccagc
     6121 agttcccgcc gaccgccgtg catgatctgc atggcggcag cgggcacttt ccaaggcggc
     6181 agttccggac gggcaaagca gacaaagggc acacaccaca accccagaca gacatagacc
     6241 cagcccagca gacacagcac ccccagcacc aacagctgca ccgcccagaa cagatacgga
     6301 gcaccctctg tgcggtgggc ggcggtttgc cccagctggt atgccccgaa caggcagagc
     6361 gtcacggttt gcagcagcag cgtccgcagc agggtgttct gcaacgccag cactagtcgt
     6421 tttccggttc ctgccggacg gcagcggggc agcagggata cggctccggt acagtgatac
     6481 cacagccgcc agcggcagac cgtccacgcc agccacgccg gcaccagcca gcatgcctgc
     6541 actatccgcc acagcactgc ctgctccgtg agcagagatg ccggcggagt gtcctgtatt
     6601 cggagcagca gtgccgctgc gaaaagttcc agcagccaca ggagcacgcc cagcagcacc
     6661 acgccccagc actcccagaa caaatgccgg tgggtgcgga gcgtctgggc ggaaaagctt
     6721 tggttctgac gaatctgcat gacaattcct ctctatggaa acactgcatc agtgcggtgt
     6781 taccttacga attctactac gcctattttt cccccgattt tcatttttat gcgaaaggac
     6841 gtggtttctt tggctgaact ccggttcacc atgcatctcc cccagcccat gaccgcggag
     6901 cagtttctcc ggcggcacaa gggtgtttcc caccgtatgc tggtacggct gaagcggctc
     6961 cccaacggca tctgctgcaa cggcagacag atccgcacca ttgacatggt acagttcggg
     7021 gacgagatcg tgcttaccga ctgcgaagtt ccctccaccc tcgccccctc tgcggcagcc
     7081 gtccctgtgc tgggagaaac agattcctac attgtctaca acaagcctgc cggcatgccg
     7141 gtgcacccct cacagggaca ccacggggac acgctgggga acgtctttgc ggcacagttt
     7201 ccgcagctgc cctttcgtcc ggtataccgg ctggacagcg acaccagcgg catctgtctg
     7261 attgccaagt ccgcctacgc cgcaggacag ctacagggca gcacccgaaa gacctatctg
     7321 gcactggtct gcggggaact ttccaccggc ggcacggtag atgctcccat tggcagagca
     7381 gagggttccg tgctgtgccg gtgtgtccgt ccggagggca agcctgcagt gacgcattac
     7441 acgccgctgc ggagcgacgg cacctacacg ctgctttccc tgcggctgga aacgggacgc
     7501 acccaccaga tacgggtaca catggcatat ctggggtatc ctctcgccgg cgaccggctg
     7561 tacggcacca gttccgaaca gtttccccac cacatgctgc actgcgattc gctggaattc
     7621 tccgatcccg aaacaggaga atgccggcag ttttcagcgg cgtgtccgtg gaattcatgc
     7681 atgtgaataa aggcaatacc gcacaaag
//
LOCUS       DAWBGR010000124         7491 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2602_length_7491_cov_3.748790, whole genome shotgun sequence.
ACCESSION   DAWBGR010000124 DAWBGR010000000
VERSION     DAWBGR010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2602_length_7491_cov_3.748790"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1768 a         2005 c         1736 g         1982 t
ORIGIN      
        1 aagcagatgg cgtgcaaagt cgccagacgc tctttgtgcg gtattgcctt atataatgat
       61 tccctcgaag tggtccattt catgctgaat gatctgtgct tcaaaatctc ggaacgtctg
      121 ctttttccgt ttgaattttc gatccagata ttccaccacg atcacgctgt accgtttgac
      181 cggacgctgt cccgtcagcg acagacagcc ttcttcggtt tcgtaacact gcctggaatg
      241 atcgacgatt ttcgggttca ccatgacgag atagtctttt ccagcctgtg ccaccagaat
      301 ggtctttcgt tccccgatca tattcgccgc catacccaca cagcggtcag cgttggcacg
      361 aaccgtatcc agcaggtctt gtattacagg ctgatcctgt ctggttgccg gctcggattt
      421 ctgactgaga aaaaatgtgt cttttacaat ttcacgaatc attttggcat ctcctcttgg
      481 ttgatctcat ctctattata ccacactttt tgcaaatacg caaaggcaac accacacaag
      541 ctttgtgcgg tgttgcctaa acaaaatgct accgcaccag atacaggcac agcagcattt
      601 ttcatatcag aatcatgttc acttccgcag atccacattc cgcacagcgt cccgcacttt
      661 cagcacgaac gccggcgaaa cggaaagttc gtcaatgccc attcgcagga atgtttccgt
      721 cagcgtagta tccgctgcca gttcaccgca gatgccgatc cacgcaccgt tggcgtgggc
      781 gttcctggca ctcatttcga tgagccgcag aatcgcctcg tggtgggtgt cgatgaacgg
      841 ttcaatgtcc ggattctgcc ggtcgcaggc aagggtgtac tgtgtgagat cgttggtccc
      901 cacgctgaaa aagtccacca taggtgccag acggtcgctg ataatggcag ccgccggcgt
      961 ttcgatcatg atgcccagtt ccatggtgtc ggaataggtc acgccctgct cccgcagttc
     1021 cgccttgact tcctcacaga tggcaaggat cttttccagt tcggacacgc tggtgatcat
     1081 ggggaacata acccccagat tgccgtaaat ggacgcacga tacagtgccc gcagctgcgt
     1141 cttgaagatc tccggtctgg tcaggcaaat gcgaattgca cggtagccca ttgccggatt
     1201 gtcctctttt ttcaggtgga aatagtccac ctgcttgtct gcaccgatgt ccagcgtgcg
     1261 gataataact ttcttccccg ccatgctctc cagcacacgc ttgtacgcca gaaactgctg
     1321 ttcttcgttg ggatagtcat tattttccag atacagaaat tcgctccgga acaatccgat
     1381 accgccggca tcgttgagca gcactgcacc aatattgtcc acactgccga tgttggcata
     1441 gatgttgacc ttggtgccgt ccctggtgac gttttccttg cccttcagtt ccagcagcag
     1501 acgctttttc tcctcgtccg ccttctgctt ttccagcagc cgccggcgag tttcctcgtc
     1561 cggttccacg aaaatctcac cagtaaagcc gtctacgatc gcctctgtgc cgtcctgtac
     1621 ctctttcaga aaatcgcttc ccacgccgat gactgccgga atgttcatat tccgagccag
     1681 aatcgccgtg tgagagttgg acgaaccgtg tgccgtcaca aatgccagca ccttgtcctt
     1741 atccagagaa acggtttcgc tgggggcgag gtcgtccgca caaatgatgt gcttttcgcc
     1801 ggaatcttcc tgcacagcaa cgtttcccgt cagattggca atgatgcggt cggaaatgtc
     1861 ccgcacgtca gccgcacgag cctgcatata ggcatcgtcc atggcagaga acatctcggc
     1921 gaagttgtcc gccgtaatgg aaaccgcata ctcagcattg actttctggg tatcaatgat
     1981 gttctgaatg ctctcgttgt agtcgtcatc ttccagcatc atcatgtgga tctcaaagat
     2041 ctgagcattg gtttcaccca cttctttcag tgccttttca tagatcgcct gtaattgttc
     2101 tgctgctgcg gctttggcag cctctactct ggctttttcc gcttcggtgt ctttcacgga
     2161 agtccgctga atcaaggtat cctgcttctg aaaaacagag atctttccca tagcgatagc
     2221 accgtacaca ccttttccgg taaacttttt catgattccg attcctcctt acgcctctgc
     2281 ggtaccgaac tttttccggt aattttccat tgccttttcc atgactgcca ctgccgccag
     2341 cggtccgccg atgtccttga cttcggtctg cttgccccgt tccagctgct tatacaccga
     2401 gaaaaagtgc atcagttcct caaagatatg cttgggcagt tcgctgacat cggtataact
     2461 catataggtg ggatcgccgt agggaatggc aatgatcttc tcatcgccca tgccgccgtc
     2521 ctccatggtc atgacaccaa tgggatagct tcgcaccagt gtcatgggca ggatcggctc
     2581 ggagcagagc agcagcacgt ccagcgggtc gtggtcgtct gcataggttc tgggaatgaa
     2641 gccgtagttc atggggtagt gcgttgccgt gaacagcact ctgtccagcg acagcatgcc
     2701 ggttttcttg tccagttcat acttcatatt gctgcccatg gagatttcca cgacagccat
     2761 aaaatctgtg ggatagatgc gttcttcatc aatgtcatgc caaatattca tggattttcc
     2821 ctcctatatg aatgtttttc cgagaactca gaggttctgc tggaagaatg cctgcatctc
     2881 tgcgaaagcg gtttcctcgt ctgcaccgtc aacagaaacg gtcacagtgt cgccgcactt
     2941 cacgcccatg cccatgactg ccatcagctt ggtcaggttt acggacttgc cgcccttttc
     3001 cagtgtgata gtgctctgga attcctttgc cttctttgcc agcagtcctg ccggtcttgc
     3061 gtggatacct acttcgtcct gaatggtgta agtgaatgtt ttcatgagta attcctcctg
     3121 taaaatatcg tttcggcaac accgcacaaa gattatgcgt aagaagcggg gtcgccttta
     3181 ttcagacagc aatgcgtgaa acagtgctgt ttgtttactt tgtgagctga atggcagttt
     3241 ctccaacaga aactgttccc ttggcaactg gtgtcaacgc tgcatagtcg tctacattgc
     3301 aaaccagcat cggcgtggtc agcttgtagc ctgcggcggt gattgcttcc atgtcaaagc
     3361 tgatgagcag gtcgcccttc ttgactgcct gtccgtcact gacatgtgcc tcaaaatatt
     3421 ttccctccag ctttacggta tcgataccga tatgcatcag gatctccaca ccgtctgcgg
     3481 aagtcatatt cacagcgtgc ctggtgtcga atacggtttc aatggtgccg tcacagggag
     3541 cgtacaactt gccctctgcg ggacgaatcg caatgccgtc accgagaatc ttctgcgaga
     3601 acacctcgtc ctccacttct tccagcggaa tcgccgtacc gttcatgtgt gtgcacagtg
     3661 tcaggcattc tccggaacca gccgcctttt ctgcgggagc agaattctgt tccggttccg
     3721 gcttggtttc ttccacagta gtttctgctt cggattctgc aaacagactg tccagatgct
     3781 ctgcttccgg cgtttccatg aagttttcca gattggactt gataacagat actttcggac
     3841 catagataac ctgtacaccg ttgcccttgt gaataacacc ggaagcaccg ctggctttca
     3901 gcatggcatc gtctaccagt tctgcatttt gtactgttac ccgcaggcgt gttgcacagc
     3961 agtccagatc ggacaggttt gccttgccgc ccagaccttt cagcaccatt gcactgacca
     4021 tgtctacagc cgcactgccg cctgcttttt tctctgcctt tgccacatta acatcgcttc
     4081 tgcgatagag tttcggctct acatcgtccg gttctctgcc cggtgttttc aggttcagct
     4141 tggtgatcat aaagtagaat acgaagtagt aagcaactgc ataaaccaga ccgacaacca
     4201 cgatccagat ccagtgggtt ttcgcattgc cctgtaaaat accgaacaat gtcaggtcaa
     4261 tggcaccgcc ggagaacgtc atgccgacac caacattcag aatgtgcatc agcatatagg
     4321 acagaccagc cagtacgcag tgtacggcat acatcagcgg agccacgaac aggaatgtga
     4381 attccagcgg ttcggtgatg ccggtgatca tagaggtcag ggctgcggaa agcagcagac
     4441 ttccgaccat ctttttcttt tccggacgag ccgcacggta cattgcaaat gccgcagccg
     4501 gcagaccgaa gatcatgaac ggaaacttac cggacatgaa gcgtgtcgca gaaacggaaa
     4561 attctgttgt agacttggaa gccagttcag caaagaagat gttctgggca ccggaaacca
     4621 tgttgccgtc gatcatcata gagccgccca gttctgtctg ccagaacggc atatagaata
     4681 catggtgcag accaaacgga atcagggcac gttccagaat gccgtagatc catgtaccgg
     4741 cgtaaccgga gcgaagcacc agcgttccca gatgagagat accggactgt accaccggcc
     4801 acagaaagaa catgatgaca ccaacgaaca ggtacaccac ggtagaaata atcggcacga
     4861 aacgagtgcc gccgaagaag gaaagcacct gcggcagttc aattttatag aaccggttgt
     4921 gcagagccgc tacgcccaag ccaaccagaa taccgccgaa cacgcccatt tgcagtgtgg
     4981 tgatacccag cacagaggtg gtggagttgg ctgccattgc ttctacgccg cccttggcag
     5041 aaatcagagc actgatcgcg gtgttcatga ccagataggc gatcgcaccg gaaagagcag
     5101 cgacttcctt ttcttttttc gccatgccaa tggaaacacc catggcaaac aacagtgcca
     5161 gattgtcaaa gacggcactt cccgccttgc tcatgatgtc cagaatggtg tagagcagtc
     5221 cgccctcata gatgatgttt cccagatgat aggtttcaat tgtagtggca tttgtaaagg
     5281 aacttcccaa acccagcaga agccccgcca ccggcagcag tgcaatggga agcatgaagg
     5341 agcgtcccac tcgctgcaat acgccgaaaa tcttgtcttt cattacaaaa gtcctcctga
     5401 aaataatgtt ctgtatcttc acgccggaat cattacttgg gcaataccgc acaaagattg
     5461 tgcggtatta ccctaggtac cagttgtaat gaaatcaagc gtttttaacg ggattcgctg
     5521 acttttttca ggtggaacgc caatgtcatc atttcgtcct ctgaaatcgt gcgtgcaaaa
     5581 tttttcagga tataatcctg taggcacttg gcacagcgat aatgtttttg gaatttctgc
     5641 cggacaaagc ttaaaatctc ctcgtcccga ctaagcacat tggagaattc ctgcgactgg
     5701 aacagccgct tcgccagata cttcagatgt accagaaacc gttcgtaaga aacggtggtc
     5761 ttgtccagct ttccctgata aaaagtatct gcaatttctg cacaaccatt caccagcttt
     5821 gtaatatccg gaacttgtcc catgttggta tgcagccgtg cgttgatgat atgcatggca
     5881 atgaagccgg cttcgtcttt gtgaagcgat attcccagtt gccggcggat ttcttccaga
     5941 caatagtttc caagagcaag ttcctccgga aaacaatcgt gaatggagtc catcagcgga
     6001 ttggcaaaat tgattccctg cttctgccgt tcgatcgcaa aactgatatg gtcggacaga
     6061 ctgatcatca gcgactcttt caacacttcc tgtgaaaact gttcagaaat atgatcgacc
     6121 agatctgatg tcagacggat atgttcgtag gggatttccg ccagacattc caccatgcgt
     6181 ttccgcaggg cagaatcagc gatcatatag attttctgga tctgtgccga atccacttcg
     6241 tcgccgcgtt ttttgctgaa tccgatgccc tttccggata tgatctgttc tttgcctttt
     6301 ttatccaaag cacatactgt gttgttattg ataactttga taattttcat aaaacacctc
     6361 aaaatggtat aaaaaaagcc aacccctata ccgcgtccat tttgcgtgca taaaagttgg
     6421 tgatgcccaa tcagacaaac ttgtcgaaag gtaacattcc aatatttcaa ttttcggaat
     6481 cctgtatcaa attcactata aacagtttac catgtagaag gatatttgtc aagatgctac
     6541 agtgaattgc cgcaggaaat ctcttttttc accaaaagtg acactttcta tttgtgcgat
     6601 aatgacaaaa attccgtttt gttccagtcc ggcaaaggcg tttctctgtg aaaacgaaca
     6661 tattctgaaa aagaattcca cgaaaaactt ctctcctgcc ttccatgatc ttgaaagtgg
     6721 atttacacaa acactgctct tacagcattg actttcaaca caaaatatgc tacaataata
     6781 caaaattcac cacaaaggaa ctacaactat gcttggaaaa acacatatga tggtgggcat
     6841 tgccgcaacg cttgccatta cgcaccctga cacactctcc ggatttgtca ccgcggcagg
     6901 gatcggagcc atcggggcac tgatcagcga tattgacgta ggcacatctt cctcccaccg
     6961 cgatgcaaac agggtcattt tcctgacgat tgccgtcgct gtcatctttc tggcagtgga
     7021 ctacttcgga aaattcggac tggtgcagac cattctgcaa aacagcagca ttgtgcgaat
     7081 tctccttggc gttgccctct ttctggtggt ctgtgcattc ggaaaggaaa cgccgcaccg
     7141 ttcgtttatg cattccttta cggcactggc agcactggac gctgccctgt gcctgattta
     7201 tccgtctatc gcagtctact ttaccattgg gtttctgtcg caccttgcga cggatatttt
     7261 caacaagaaa aaagtccgcc ttttgtatcc gatgaaaggc ggcgtatgcc tgaatctctt
     7321 tcacgcctac ggcactgcca atacagtgtt tttcattgcc ggctgtgcgg ttacggcttt
     7381 ggaaacgatc ctgctgttgg tgcggatcgc cgggaatttt acagcgtgag gaataatttt
     7441 aaggcaatac cgcacaaaga gcgtctggcg actttgcacg ccatctgctt g
//
LOCUS       DAWBGR010000125         7483 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2607_length_7483_cov_3.576467, whole genome shotgun sequence.
ACCESSION   DAWBGR010000125 DAWBGR010000000
VERSION     DAWBGR010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2607_length_7483_cov_3.576467"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7038..7047
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2074 a         1738 c         1586 g         2075 t
ORIGIN      
        1 caccgcacaa agcttgtgcg taaggtgcgt tgtcagattt ttcggcagat tgcctttaca
       61 ccgtcagcgt aaaatctcgc tgaaaagacc tgcatttcat ttttatgcga acacattcta
      121 gtataacaca tcgttttaaa aaaagcaaga aaaaattgat aaagtgaaga acaactacac
      181 tttgaggtta gggtgtcaag ttttattata ctacatcaga ggtaaaaggc gttttcagag
      241 gatagcaaac agcctgagat tccaattgta gaatctcagg ctgtctttcg tttgtatgca
      301 gtgagaaaag tgagatggtt ttggcatcat gtttatcggg aaacactgaa taaagcttgt
      361 gcgtaagatg cgttgtcaga tttttcggca aattgcgtgc aaagccgtca ggcgggcttt
      421 atgcagtgtt tcctttgttc atgcagtttt ccgaaattct tccacaagac catttctctg
      481 cggactgatt ccgctttcgg taaaatcgat cagcggttcg ctcaaagctg ttacatcata
      541 ggcaataccg cattcacctt actctgtcgt atacaacgta tatgcttcgc cagtgactac
      601 ctccgacaat ccgtatacca cactgctgat cactgcttcg ccctgattgg catagacttc
      661 gatcaaattg gaatctacat agatctccag ttcacagccg tccctgagta ccggtgtttc
      721 cgcagtcaat cgatatttgc cgtctttcac aaatacagca gagcgatctg tgaaaagctt
      781 gtcctgttcc cgccagatcc ggtatccgcc aatgttgata cactcgccgt ttttcagcga
      841 ggtctttacc agatagccag actttcccgc ctgctccggc gaagagatct ccttggtaaa
      901 agcgttttgg atatgcggat gaggacggaa ataaatgcga tcgtacttca cagaaaccac
      961 tcttggaata cacatcatgc cgttccactt accatctact gcctgcggca ttctcatcca
     1021 tgcaataaca acacgattgc catcttcatc agtcgtactc tgcggggcat agagatccaa
     1081 cccatagtcc agaaactgaa cgtcatctgc aagctgcatc gttccggtcg cttcatcgaa
     1141 tgtggagaac agacagatcg ctgcggaatc atacgctttt ccatcgttca aaaaagccat
     1201 gggagaaaac accgtaatcc ccttaccgtc cacctcgaaa tagtccggac actcccacat
     1261 ccagccgaag ttgtcctttt ccacgtgact gagatacttc cagttctgca aatccctgct
     1321 ctgataaatc agcagtctgc ccttgtgggc aacagagctt cccagcacca tgtaccacgc
     1381 atctgttcca cgccatacct tcgggtcacg ggtgtgcgtt ttgctgccga tcttatcttc
     1441 tgcaatcgtc ggaatgatcg tcttcttttc ttgaaggtta tcaaaaatca taccgtccgc
     1501 ggaagagatc atcatctgtg ccgagagaaa ctgatcgttc cgacagtggt taatgttctc
     1561 cggatcctcc tccagatagt tgacaccggt atagtacaag tacatcgttc cgtcctgctc
     1621 cacagcactg ccggagaaac agccgctgcg gtcatcggtc ttggtaggat acagggcaat
     1681 tcccttttcc tcccagttca ccagatcctt gctgacagcg tgtccccagt gcattctgcc
     1741 ccactgcggt gcgtagggaa agcactgata aaacagatga tactgtccct gataccagat
     1801 aaatccgttc ggatcattga tccagcaatt cggggctttg aaatgaattt tctgcatagt
     1861 atcctcctaa ttagaaaagg ctctcttcgg tttgtgtatc aaaaaagtgc attctggacg
     1921 gaacaaaggc aaaatcaatg gtatccccgg gcttgctgat ctcatatttg gatactctcg
     1981 ccaccgaggc agtgccaccg aagttaaagt acaggttgtt ctcatttccc ataacttcta
     2041 cactatccac cgttgcagac atgatattat ccttgtaaag atccagattc tgcggatcca
     2101 gcttgatgtc ctcgccacgt atgccgagaa ccagcgttcc ctgtctgccg tttagtcttg
     2161 tcacaacaga ttccggcagc tgcatcttac tgccgtcgga gaaagtcatg gtattccctg
     2221 ccacctttac ttcatagaaa ttcatctgcg gcgaaccgat aaatcctgcc acaaacttgt
     2281 ttgccggatg ctcatacaag tccatcggct tgcccacctg ctggatcaca ccgtctttca
     2341 taatgacaat gcggtctgcc atagtcattg cctctacctg atcgtgcgtc acataaatgg
     2401 tggtactgcc cagctgccgg tgcagcttgc tgatctcgct tctcatgctg actctcagct
     2461 tagcgtccag attggaaagc ggctcatcca tgagaaacag cttctgatcc ttgacaattg
     2521 cacgtcccaa tgccactctc tgccgctgac cgccggaaag attcttcggt ttccgataac
     2581 ggtattccga aagtcccaga atatcgattg cccagtccac cttcttcttg atttccgctg
     2641 ccggtacctt catattcttc aaaccatagg aaatgttctt ttccactgtc atgtgcggat
     2701 agagggcgta gttctgaaat accatagaga tgccacgatc tctcggcaaa actgcattca
     2761 tacgctttcc gtcaatgtag aagtcgccgc tggtgatttc ctcaaatccg gcaatcatac
     2821 gaagtgtggt cgattttccg cagccggacg ggccaacaaa ggcaatgaat tcaccttctt
     2881 caacggaaag actgaaatcg gttacggaat ttttatctgc acctgcatat ttcttacaga
     2941 tgttcttcat ttcaataaag ctcataagtt aaccctcctt ggaacctgtg gaaacaacac
     3001 cctctacaat ctgcttggag aaaagggcat agatgataat aaccggaatg gtaatcaggg
     3061 tgatgacagc aagcgtctgt cccatggttg tattgtcgtt ggatgtgata gagttcaaac
     3121 cgatctgagc ggtcagaaga tcgctggacg tgaacaccag tgacggccag aggtaatcat
     3181 tccatacatt caggaatgtg aaaacactga cagttgcaac gactgtttta gacatcggca
     3241 gcaccattgt aaagaaatac ttcacaggat tgcagtgatc cagctgtgca gcctcccaga
     3301 catcatcggg caggctgaca aaaacctgac ggaacaggaa tatattaaac ggattgacga
     3361 tcatcggaag cacataacca aaaacggtat tcagcagtcc cattttggaa acgaacataa
     3421 agttgatggt gataatggtt tctgtcggaa tgatcatcag catcatgatg atcgccaacc
     3481 atctttcacg aaacggaaac ttgatcttcg ccaaggcata acctgccagg ccgttgacga
     3541 tcacaccagc cacgatcatg attgctgcat agaacagtgt atttgccata aaccggagga
     3601 atcttgtgtt gaacaaaatt tccttatagt tatcgaaaaa tccctttccc ggtgcgggcg
     3661 gaataaatgc actgacggaa ttcatatctg ctgtgattgc agcgtccggt ttaaacgaat
     3721 ttgcaatcat ccaaatgact ggaaacagaa agaataagga caacaccagc attactgcaa
     3781 caaggattcc attagcgatt ttttgctttt ttgttttcat gtttctcctc cttgtccttt
     3841 gtcaaaaatg tttgaatcat agtgagtatg acaacgaata cagtaaagac gattgccatt
     3901 gccgaagcaa gtccaatttc ccagtttacc gtaccggttt cgtaaatatc atagacaatg
     3961 gtataggtcg aatgagacgg accgccgccg gtcattacca tcggctgcac gatcatacgg
     4021 aatgcaccaa tggttactgt gataaagaca aatacacaaa gcggcttcag ttccggcagt
     4081 gtgatattct tgaatttgct ccagccgctg gcgtgatcca tttccgcagc ctcatagagt
     4141 gccggattga ttgcctgtaa tccgccgagg aatatgatca tctgataacc gacaccttgc
     4201 cagacactca tcagtgcaat ggaaggcagt gcctgattgg caccatagat aaacggctgt
     4261 gcatcaatgc caaagtgaga gagaatcgtg ttcagaatac cctccggact atagatctgc
     4321 atccagagat ttgaaacaac tgccagggac atgaccaccg gaataaaaaa tgcgaccttg
     4381 aagtatttct tacaaactgt gactttattg atgagcagtg ccagtcccaa tgctcccagt
     4441 gtttgcagcg gaacaatgat cagtacaaac ttaacggtat ttccaaaagc tgtggtaaac
     4501 aggtcgtcct taaaaatggt actgaaattt tccagtccta caaaatgtgt cagctttgga
     4561 tttaatgcaa aataatctgt aaagctgtaa attaatgtga gtatcatcgg aatgaacagg
     4621 aacaccgtca ttaaaaccag tgccggtccg aaaaaagcca ttccgagaat ggaattcgat
     4681 ttctttttca tgttatcttg tgtaacgctt gagctttgcg ttgatccttt ctacagattt
     4741 atccatttcg tttgtgacgt ttttgccgct caatccaacg tcctccagaa cctgctggaa
     4801 cgatgtgctg acctgcggat aaacaggtgt tttcggtctg ggatgaccgt aatctctcag
     4861 ctgttcgtaa agtgccttat aattttcgtc ttcctcaaaa gtggtgatct ggtcatatgc
     4921 tgcataagtg gaaggcatac tcttggtttt ctcatagaga agaacgccgc tgtccacacc
     4981 gctcatccat tttaccagct ctgtagcggc ttcaatgtgt tccgtttttg tagtggcagc
     5041 ataggcccag gaaccagtcg gcgtatatct gccgccgtcc cagttgtcac tgacaatata
     5101 cggtgcaacg ccaaggtcga tgtcgctgta gctctgatag atcgtgttga cttcccatgc
     5161 accgtcaaac ttgaacgctg cacgtccgct ttcaaacagg ttttcaatgg gtgttgctga
     5221 catatacttg ttttccacca gtcctctgaa gtactggaac gcctccgcat ttttgtccga
     5281 gttgaaatac ccgtctgcgg taagaccgtc ctcactaatg agatcgccgc cattggacca
     5341 gataaacgga gcatagtaat aaatgctgga ttcgccgacc ggaaaggtca tgtccaatgg
     5401 atacccgttt ttgctgtcca tgatcggttt cagctgctcc aaaatatcca gaaatttcga
     5461 gaatgtccag gggtgatcct tatcgggcac ttcaattcct gctttctcca gaattgcctt
     5521 attataataa agacctacgc tggattccat ggcaccgagt gcatacagtt tgtcatcgac
     5581 cgttccctga tccagaatgg aatccagata agccgatttt tcttcctcgc tcaattcggc
     5641 aagcggctga ataatgccgt ttgctgcata ggcagaaata ttcggtccgt ctacggtgat
     5701 gacatcagga agatctcccg acataaccga agcattgatc ttatccgaat aaccgccgcc
     5761 gctgtcattt ctcggaataa attcaatgtc tgcaaaatag gttccgtcat acttttcatt
     5821 gaagcgttcc accgacgctt tataagcttc gccttccggt gtgtcctcaa tggtatgcac
     5881 ccacatgctg atgtactgct ctccctcatc atatcccttt acatcaccag gctttacggt
     5941 tttgctgcat cctgtaagcg gaactgcact cgccagaatt gaaagagccg tcagaaataa
     6001 tggtagcttt ttcatcgctg tttccctccg ttctttttaa gtaaccggtt actttactga
     6061 ttacatcata ccatggaacc ggttacttgt caagactttt tctgattttt gtagcaattt
     6121 ctaaaaatcc tgaaaatgtt ttgtgcaatt caacgaaacc ggttacttta ctgattcttt
     6181 tgttgattca aaaaccgttg tgtgctataa tgaagagaga aacagggagc gtgaaaaatg
     6241 gcgaaaaaaa tgtaaccttt gcagatattg cagcatatac cggcttttcc aaaacaacga
     6301 tctcccgata ttttaaggca ataccgctga gaaacttggg cagagttttg ggagaattgg
     6361 aaattatgta cctctttttg atgttactcc atttgaaaac gagtggacgc gacgtggtcc
     6421 atggattcaa gcgactttct gggttcttac aaaagacatg ataaaaaaag tcagacgttt
     6481 caaagcagcc aaacattgtg agccgtctat tgaaaaatga actgcatttt atatcgtgat
     6541 ataagtgctg ccgttaaaaa acgtaaaaaa aattaagctg aaaaacgagg gagtgatatt
     6601 gtgcatatct ggacacttga gaattggaaa tataacttca caaacattca atcaagaaga
     6661 agcgggctta gattccgttt tacccctgac gttaatacag aagtaagagg agcgtgcctg
     6721 aaatttggaa agtggttgag aaaagagtat tttttcccaa taagggttcc agtgtatgtg
     6781 aaagggaaaa aatacattaa ggcaatggac ggcgaaatgg tatatggtac ttttttccaa
     6841 ccatataaca agacatatga gccatatata cgtgtggcaa cggggagtta tacagacaat
     6901 tcgataacat taggacgaga cgatgcttta gctctaatac tagaaacaat agcacacgaa
     6961 ttgacgcatt attttcagtg gattaacgat attgggctca caaaaattgg atatgagcgt
     7021 caagcttctt cttatgcnnn nnnnnnngat gaatacgcca atgtggttga ccatccttaa
     7081 aaaaatcaag aaagaatcaa aaccaatatc cagcggtctg gatatatctg caaaaataaa
     7141 actgagtcag tatgagaaac tggggcagag tgcttggcaa gctggagatc atgtaccccg
     7201 acagacagac tgactgttgg agaaacttga caaattacgg tatttatttt aatactgtaa
     7261 aaaattgagc ggttgttctt caagccgctc gcaatatcgt tttttgtcac tgttgttgaa
     7321 tttacagaaa ttttttcgca gagactcgat aggagtgttg gttatgaact ttataaaaaa
     7381 aagaaaatgg gtgcttttca ttgccttttt cttgcttttt atctgtatcc ttttattgtg
     7441 caaaattgaa tcaaatatgc agaaagaaag taagcaatta aac
//
LOCUS       DAWBGR010000126         7401 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2640_length_7401_cov_4.054724, whole genome shotgun sequence.
ACCESSION   DAWBGR010000126 DAWBGR010000000
VERSION     DAWBGR010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2640_length_7401_cov_4.054724"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1972 a         1843 c         1749 g         1837 t
ORIGIN      
        1 aaagtaaaca ttccactttt tagacttttt ctcatatcac tcagattact gtcattgtct
       61 tcaataatga ctcctttttc ggtttgcttt gggatataat aatttccttt ttcagaaatc
      121 acgtactttt tcatgaagaa attcaaggat tcgaggaact ggaaactgtg tgtgacaatg
      181 agaatggtca aatcaaaata tttttgaaga gcaacaataa gttcggcgta gataatttgc
      241 cattcagaat ggagatggat ttcaggctcg tcaagaataa gaatatcttt tttcttcagt
      301 acacctgttt caagcattct ttcgatgaga gcaaaagatt taagaccagc tgaaatgttt
      361 ctgaagtcga aatccacacc attttcactg taataatact taccatgact gattacagta
      421 tcaccggcat aggctctttt caaaaccttt ctgatctcat cgagttcttt tttgtttata
      481 acggtttcaa gaatgttgga cattgagtct tcatttatgt ctgcctgtgc ttttattatt
      541 gcactaatca catttctgtc cataggattt agggcagata agaaccatga tacattcgtt
      601 aggttaagcc aatccagtac aaatgggtta ttgatatagt atgcagaatg ctcaatgggt
      661 tcatctaact cacaggagca ggatttttta tttaatagaa ttgtatcgct ttttcccttt
      721 atttctgcag taacagaact tttgcctgtt gaattcaaat tcttgatctg cccattcata
      781 acaagattaa agtagcggaa tacatattcg ttcaaaatat ctttttccgg taggtttaag
      841 tattcaacca gagaagctac cttgttcttt gtaactttta tggggtaatt ttcctcaaga
      901 tattttttta catcttcaac agaaaaatct tcagcctgag aagtgataaa ggacattgtg
      961 ctatcattgt caaggagtct aaccggcaca tccatggagt ttatattcga caagataaaa
     1021 ttcttaaacc ttgaatcttt ctgcatactg atttttgtct tataatcata tagagcatta
     1081 taaaaggaaa aaagcgactt gccaatcgta ctcttaccag tgttattgtc accgcaaatg
     1141 acagtgatcc catcaagttt tatatccgct tttttgattt taccgagatc cttcaacttg
     1201 aaatccatac aatcactcct cctcttctta ctaaaaagcg aatcatacaa catatcatgc
     1261 tgcatcaaaa aataccgcta tataatatta tgagcagata tgctgtaaat actcgtataa
     1321 accaaaaaag cactcaccac agagggtgaa tgcctttctt tttttgttag ttacctgtta
     1381 gttacgtgtt agttaccgcc taaataacac cctattttag gcaaaatcga aacgacaaaa
     1441 acgacgcaaa acaggtgatt ttcagcactc ccaaacggga taaaattatc tctttgagaa
     1501 ctgcggtgca cgacgtgcag ccttcagacc gtacttctta cgttccttca ttctcgggtc
     1561 acgagtgagg aagccagcct tcttcagaac cggacgcagt tccggactga ataccagcag
     1621 agcacgggag atgccgtgac ggatcgcacc agcctgaccg gtgacaccgc cgccggtaac
     1681 tgtgcagata acgtccagct tgtccatgtt gtcagtcaga ttcagcggct gacgaacgat
     1741 cagcttcaga gtatccagac cgaagtattc gtcaatgctt ctgccgttga tcgtgatgct
     1801 gccgttgccc ggatacagac gaactcttgc aacagagtgc ttacgtctgc cggtaccata
     1861 atagtatttt accttagatt cgtacatctt ttctgctccc ttctcttaga actcgcaaac
     1921 ttccggcttc tgagccgcct gcttgtgctc tgcacccttg taagcgtgca gacgcttcag
     1981 ctggtttgca ccctgagtgt tgtgcgggag catgccctta acagcgatcg tcattgcttc
     2041 atctgctttt tcttccatca taaccttata ctgtgtcttc ttcagaccac cgatccaacc
     2101 ggtgtgccag taatagaact tcttttccag cttgttgccg gtcagaactg ccttgtcaca
     2161 gttgatgatg attacgttat caccgcagtc aacgtgcggt gcgaatgtgg gcttgtgctt
     2221 accacgcagc aggtgtgcag cctttgcagc gacacggccc agcggcttgc cggcagcgtc
     2281 cagcacatac cacttgcgat ctacttcgcc agccttcggc atagttgttg ccatagtcaa
     2341 ttcctccatt gaaacttcta tcaaaaataa tctcgatcca gccaaaaaag caggatcagg
     2401 cagagggcag ttgggttaga ctgcactgcc ggcatttcgc aagattataa ccggtctctc
     2461 tgacagtaca agaactatta taccatagat tcggcggaaa tgtcaagcgg aaatccgcag
     2521 ttttttttcg attttcagag gaaatccgct gtggattttg tgcatattgc cattttgccg
     2581 ccgaaaccgg actgccggct tgcaattttt caggaactgc gatataatag tatatcagaa
     2641 catgccccgc caaactgggg atatgctcgg cagatctctg ccggaacgga ggtatcagaa
     2701 aaatggcaca atctaagacg accggcggcg aggggatcgt ttacctgaca cgccgtgcct
     2761 atgaaagcgg tgagatccgc ctgctgctgc ggacagatgc tgtctgtgca gaacgggata
     2821 ccggctggca gctgctgcag ggcagcgaaa ccggactgga actgtccaat ccggaaaacg
     2881 ggctgctggt atccgtcgcc agagcactgg aactggaacc gaagctgaca ccgcttctgg
     2941 gggtatcctc tgctccgtgc agggcggcat atgcctatga cgaaaaaaac ggcgggtttg
     3001 tccccacggc gtatcccgcg gacattccca ccgaatttta agaaaaagcg atttacagaa
     3061 ttgccggcag tgctccggta ataatatgac ttgggtctgc gttggcaagg atcatatagt
     3121 acaccagaca cgcaagcata catataatga aaaaagcgac aacaacacgc agtcggaaac
     3181 gcagaaacgg tttcggcttg tgaatatcat caacacggtg cagctgcttt tgtcttttac
     3241 gcattgcgga actcctttca acaggtttga aacgtatgct actattataa acgataaagc
     3301 ggcattttgc aagtgctttt tgcaggggca acactgaaat ctttcggtaa ttccaacaaa
     3361 ggagaaaact tatgtgtggt tttgtaggat tcaccaatca tattgacaac tcgaacgtgg
     3421 ttctcggaga gatgctggat aagatcaagc accgcggacc ggacgcagag ggtacttata
     3481 ttgacgaaga cattgcactg ggacaccgcc gcctgagcat catcgatgtt tccgaggcag
     3541 gcacacagcc gctgtacagc gaggacggca atctggcact gctgttcaac ggcgagatct
     3601 ataactatca ggagattcgt gcccgtctga tcgaagccgg ctatcacttt gccacacaga
     3661 cagactccga ggtactgatc tatggctatc aggaatacgg tgcagacctg ctgcaacagc
     3721 tgcggggcat gttctccttt gtcatctggg ataagcggga gaaaaaactc tttggagcac
     3781 gggacttttt cggcatcaag cccatgtact atgcccagat gggagaaacg ttcctgttcg
     3841 gctcggagat caagagcttt ctgtgccacc cgcattttaa aaaggtactg aacaccgccg
     3901 cactggaaaa ctatctgacg ttccagtact cgccctgtga cgaaaccttt ttccaaggag
     3961 tgtataagct gccgccggca cactatttca cctatcagga cggcaaattt accatgaagc
     4021 ggtactggga cgttcacttc aaggcagacc agaagccaga tctggacaca tgggtgaacc
     4081 gcatttcgga tacgttccac gacagcgtag aggctcacaa gatcgccgat gtagaagttg
     4141 gctcgttcct gtccagcggc gtggattcca gctatgtggc agccattgct aatgtggata
     4201 agacattcac ggtcggcttc ggtacagaag aacgctataa cgagatcggc tgggcaaagg
     4261 gcttctccga ggcaatcggc aagaagaact ataacaaagt catcacaccg gaagaatact
     4321 gggaaaagct gcccatgatc cagtaccaca tggacgaacc gctggcggat ccggcagcga
     4381 tcgccctgta cttcgtgtgc aatctggctt cccagtccct gaaagtcgtg ctctccggcg
     4441 agggtgccga cgagatcttc ggcggctaca acgtctacag cgaaccgggc gggtctgcct
     4501 atgataagct gccctgcgga ctgcgtcgcg gcatcggaca catcgcagag aaaatgccgg
     4561 cacaccgcgg cagaaacttc ctcgtccgca agggcaagga tctggaagaa cgctttatcg
     4621 gcaacgccta tatgttcaca ccggaggaac gcaaggcact gctgaagatc cgcaccaacg
     4681 ctcccgatcc catggcgatc accaagccgt tctatgacaa ggtgcaggat caggacgatg
     4741 tgacaaagat gcagtatctg gatctgcaca tgtggatggc aggggatatt ctgctgaaag
     4801 ccgacaagat gagcatggcg aattcgctgg aactgcgtgt gccgtttttg gataaaaagg
     4861 ttatggaact ggcagagcag atccccacac ggtaccgcgt cacccgcgaa gccgttaccg
     4921 acgaaaagac gccgtatatc accaagtacg ccatgcgtct ggctgccaaa aaggacactc
     4981 cgccccagac tgcaaagacc gcagccaaga agaagctggg ctttccggta ccgattcggg
     5041 tttggctgaa agaagacaaa tactacagca ttgtgcggga acgctttgag agcgacgagg
     5101 caaaccggtt tttccacacc gaaaagctgg tgcatctgct ggacgatcac cgtgcaggca
     5161 aggcagacaa cagccggaag atctggacac tgtatatgtt cctggtatgg taccagatct
     5221 attttccgga ggcatgcgga gcataaaggt aaaggagcgg cagaacaaaa atggcaagtc
     5281 atgtaaccta tgcctatatc cggcagaatc ccgatattcg ggaatatatc cgccgtgccg
     5341 atatgtccct tgcggcgatc ggctataccg agcattcctt tgcccatgtg gaaaaggcgg
     5401 cacacaatgc ggcaatgatt ctggaaaccc tgaactatcc gccgcggcag gtggaactgg
     5461 cgaaaatcgc cggctttctc cacgacatcg gaaacgtcat caaccggaac gaccacgccc
     5521 aaagcggtgc agtcatggca tttcgtctgc tggaccggct ggaaatgccg gtagacgaga
     5581 tctgttctat catctcggcg atcggcaacc acgacgaggg cactgcccag ccggtagatg
     5641 ccatttccgc tgccctgatc attgcagaca agacggacgt tcgccgcagc cgcgtgcgga
     5701 acaccgactt tatgactttc gatattcacg accgcgtgaa ttatgcggtg gaatactcca
     5761 acctgcattt cagcgaggac aaaaagtcgc tcgttctgga tctgaccatt gacacggaga
     5821 tctcctctgt gctggaatat ttcgaaattt tcatggaacg catgttgctt tccaagcgtg
     5881 ctgccctgtt ctttgataaa aagttcaaga tgtacatcaa cggcagcagt attttgtaaa
     5941 gacaactccg ctggtttcca gcggcattgc cataaattcc cgaagccgat gcacgacttc
     6001 ggagaaaaca acggaggtac aatatcatga tttcaccgta tccgcatctg attgatttga
     6061 acgactatcc tgtttcgtgg tggaaacagg tggtggatct gggggaacag atcaaaaatc
     6121 accccgaaat ttacgccggt gcatgcagca acaagatcat ggcgaccttg ttctatgagc
     6181 cgtccacccg cacccagatg tctttccaga ccgcaatgct gcgtctgggc ggcagactga
     6241 tcgggttcga caatccggca aactcctcgg tatccaaggg cgaaactctg aaagatacca
     6301 caaagatcgt cagcggctac gcagatattc tggtgatgcg tcatccggta gccggtgcgg
     6361 caaaggcagc tgccctgtcc gcagactgtc cggtcatcaa tgccggcgac agcggacacc
     6421 tgcaccccac ccagaccttg accgacctgc tgactctcca gtgtgaaaag ggtagactgg
     6481 atcacctgac cattggtctg tgcggcgacc tgatcaacgg cagaactgtg cactctctgt
     6541 tgaaagcaat gtcctgcttc gagggcaacc acttcattct gatctccaca ccggaactgg
     6601 gactgccgtc ttatgtcaag gatattctga acgccagcaa ctgtgacttt gaggaggttt
     6661 cttctctgga agaagccctt ccgaagctgg acgttctgta catgacacgg atccagcagg
     6721 aacgttttgc cagccgcgag gaatatctgg cacagaagga cacctatatc ctgaccacca
     6781 agaagatgca ggcagccaag aaagacctga tcgtgctgca cccgctgccc cgcgtggacg
     6841 aaatcgaagt ggctctggac gaagaccccc gtgcgatgta cttcaagcag gcaaagtatg
     6901 gcatgtacgt ccgcatggca ctgatcatca ccatgctgaa tctggacacc cacaccacac
     6961 tgctccgcgg caagacgttc cccggtgcag tctgcaagaa tcccaactgt attacccaca
     7021 ctgaaaccta tctgcctaag agctttattt ccctcggaaa ttctctgttg gagtgtgagt
     7081 tctgcaatga acggctgctg atggaacact gatcggaaca gtatttttcc tgtcatttct
     7141 aaaaagcacg atttcccacc gcggaaatcg tgtttttttg cacaaaaata tagatgaaaa
     7201 aagatgaatc tatacaagtt tttcacgaat ccggttaatc ttttgtaaat ccccttgcca
     7261 agaaaatcgg tttgtgctat actaaagggg aagaaaaggg aacgccgtca gcaattccat
     7321 gcaaacttta tgcagcgttt ccttagggga aacactgcat aaagcccgcc tgacggcttt
     7381 gcacgcaatc tgcttgcaga t
//
LOCUS       DAWBGR010000127         7338 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2666_length_7338_cov_4.169024, whole genome shotgun sequence.
ACCESSION   DAWBGR010000127 DAWBGR010000000
VERSION     DAWBGR010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2666_length_7338_cov_4.169024"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1486 a         2134 c         1785 g         1933 t
ORIGIN      
        1 cgtacaaagt cgccagacgc tctttgtacg gtattgcctt tattcaagtt gaaaagcatt
       61 actcataccg cagggcatcg atcggattca gattcgaagc cttgatagac gggatcagtc
      121 caaagacaat gcccaccacc atagagaata ccacggaaac cacaatggac agcgaactga
      181 tcgccaccgg caccgaggca atcttggaga tcagctgtgc cagagcgatg ccgattccca
      241 caccaatgat tccgccgatg ctggtcagca cagaagcttc tgtcaggaac tgtgccagaa
      301 tgctgccctt tttcgcaccg attgcctttt tcagtccgat ctcgcgggta cgttctgtta
      361 ccgataccag catgatgttc ataacaccga taccgcccac cagcagagaa atgctggcga
      421 tccagatcag catggtgttg gtggaggaac tcagtttttg cagggacttc gcctgtttca
      481 gcaagtcctg actgtcatat ttgatctgat tgctgtcccc ttcttttccg acgctattgg
      541 tgcgaatcgt ggcgttcatc agttccgcca ccttttttcc cactgccggc atgctgtcag
      601 tatccactgc ctttgccaga cagttttccg gttcatcata ccgatacgcg atcggccacg
      661 actgaccggg gataaacatc ttgccgccgg aagtgcccat atacgtccag tagtcctcct
      721 cggaattgat gaccggctca aagctgctga cctctgtcac gacgccaacg accgtaaagg
      781 gttccccgtt gatgtcgatg acggaaccga tcgggctctc ggaggagtac aggctcttga
      841 ctgcggtgct gtccagaatc actgccttgg cgttattggt atattccttg ctcaggaatc
      901 ccctgccctg ctgtacttca tagcctgccg tatcgaaata ggcagcatca atgccataca
      961 tggagccact gaactgggtg ttctgataat acacattgtc gcagtaggaa cgtgtacggt
     1021 aaaacgaagc agattccaca tttttcaggc tggtgatctg gtcccgcact tccgcggaaa
     1081 ccacctgtac cccgtccggt gccgcctgat agctgaaatc atagggactt tccccctgat
     1141 acagctgcac attaacagtg ttgttgccgg aaccgatcag attgtttttg atctgctcat
     1201 tggtgccctt aatggtagac acaatggcaa tgatcgccgc aataccgatg ataatgccca
     1261 gcatagtgag aatggaacgc accttatggg acaaaatgcc ctgtaacgat aaccgaatat
     1321 tttccagcat aatatgtccc ccttagtatt ctgatgagcc gttggtttcg ttgaccaacg
     1381 ctccctctac ggcatacttg ccatagggga aacagatata atcttcctga ctgacaccgg
     1441 agagaatctc aatggcatag ccgccgttga tgatcttgcc ggtctggaca ttctgccgtt
     1501 ccagcttctt gtccttggat tcccgcagca catagtactg tccgttttcc tgccgcacat
     1561 agctgaccgg cagatagaac gtatctccga catcgtccgc cgcaccggaa aagctcaggc
     1621 tgacatagct gcccactgac aggctggtgg aatcagtcac tgctgcctta aacggataag
     1681 cactgttgtt cgggtttcca ctgccgttct gattggtgta ggaggtcggt gtcttgtcga
     1741 tctccgtcac ctcggcggtg agcatttcac cggtatccca cgagttgaca gtcaccgttg
     1801 caccgacagt cagcttgtcc agattcattt cgctgacagt gccctgcacc ataacgccgc
     1861 cgctgccctg taccaccaga tacggttcgt ttttctccag cgattccact gaagccgcca
     1921 ccttggtgac aacgccgtcg atcttcgcca gttcctgtcc cttttcctgc ttttctgccg
     1981 cagcatcata ttcgatctgt gccttcttct ggtcgatctg tgccgactga atggcgatct
     2041 gctgctctgc caccttctgt ttcagctggg cacgggtata gacatagttg tcattgtccg
     2101 taccggaaga tgccattgga gtgactgccg ccattggcat tgccgccgcc ataggctgca
     2161 ctgccgcagt cggatctgca ctggtgtctg tatctgccgg ctgggtgtcc ggctgggtat
     2221 ccggagatgc tgtgctgctc tccggtacag taccagtact gccgccggta gtgccggaag
     2281 aagccggttc tgtttccgcg ggggtgttat tggcagggtc ataggagaac gtaccgccca
     2341 cctgcaacgt gccgtaccac gttccgccgc cggagatctg cacaccgccg tcctcggtca
     2401 ccttgatgtt ctgtcccagt gtccagtcac cggtagtggc cagctgatcg ccgcgtacca
     2461 gccagccgta gagcacgctg ccgctctggt catacaccac gaaaacggca cactgctttg
     2521 ccgttttcag ctgctccaga gctgcctgtg tcacaacagt ctgaccggtg caggcaaact
     2581 gatagggatc gtccggcgtg ccggtaccgg ctgccgcctg tgtcagagcg atcactgctg
     2641 aaagtgtatc cttgggagaa tccggtgtat ccggcgtgtc cggttcgtct ggttcgtccg
     2701 gctcatcggg gtaatccgga tcgtccggca ccaccggtgc ttcttctgat ggacgcagct
     2761 ttttcagacg ttccagctct ttctgagcct gccgcacatt ttcctgtgcc accgccagtg
     2821 ccgtctgttt ctgggcaact tccagcccta ctgctgtcat atcatagtcc agcagcggtg
     2881 tgcccttggt cacatggtcg ccctctttta ccagcacctt tgcgatcatc tggtcgtcgg
     2941 agggcgtgac attctgcaca tcaccggaaa ccacctgccc gtccatagag agatcatctc
     3001 cccaccagta ctgggtcaga tttgccaccg gcatcacatc tacgcggttc tttttcgtct
     3061 gctgtgtccg gtaaatcacc gtaccgccga cagtgccgcc ggcaacgact gccaggcaaa
     3121 ccgccactgc aacgatcttt tttccttttg tcatgaagca tcgccctcct gttctgtccg
     3181 gcgttctccg aagccgccct gctccagcat gccgtcccga atggtcacga tccgctgggc
     3241 atgccgggcg atctccggtt catgggtaat catgacaatc gtcacgccct cgctgtgcag
     3301 ctgggcaaac aattccatga cctgttcgcc ggaacgggtg tccagtgcac cggtcggctc
     3361 atccgccagc agcagacggg gctggctcac cattgcccgt gcaattgcca cacgctgttt
     3421 ctgtccgccg gaaagctgag tcggcttgaa ctgtatccgt tcttccagcc ccacacgtgc
     3481 cagcatttcc tttgcccgtt ccagccgttc ctttttccgc acgccggcat acaacagcgg
     3541 cagtgccacg ttttccagca cagactggcg gggcagcagg ttgaagttct ggaacacgaa
     3601 gccgatcttc tggttccgta tcgtggaaag ctggttgtcg ctgcactgtg ccatgtcctg
     3661 tccgtcgaag aaatagtccc cgctggtctg cttgtccagc agcccgataa tgttcatcag
     3721 cgtggatttt cccgaaccgg acggtcccat gattgccaga tattcccctt cttccacctg
     3781 tagctgaatg tggtgcagaa cggggacagg ttcttttccc tgcatatagg atttacagat
     3841 gtcccgcaaa tcaagtacca tctgccgcac cgccttccgc tgtatctgct ccggcttcat
     3901 ctgccggcat ctcgtcaaca ccatcgccgg catcttctcc tgctccggag atcacgctgt
     3961 ctgcggtgtc gccgtctgca ccgtcgcctg ccatgccggc ttcatcatct gctccgccga
     4021 tcacgccttc gtccacaggg tttccgtttt cgtcgtagga aatatcctca ttgctgttgt
     4081 cgtccacggt atcatctgtg ccgtcatctt ccggtgtggc ttcgtcaggg ttggtcgtgg
     4141 ttttcatacc ctcctgatag gaatccgacg ggaatgcgat catatcgtcc tccgacagac
     4201 cacttttgat ctcgcagtcg cccatggtgt cgtctgtctg tcctacttcc acttcccgtt
     4261 tttccagacg gtctttctta tcggcagccc agacataggt cttgccgttt tcatcagtct
     4321 gcacataatc ggagtacagc cagatgccgg ttttttcaat gctttcatcg gctccggtat
     4381 ctgccgaaac cagcagatgc tgccccagca tcagcccgtc actggaatcc agcgtcacat
     4441 aaaaaggata cttggaagcg gtggtcatct catcgctgcc gccgtagtac atgttgttgt
     4501 tctggctgtt gtcggcttcg gtagagatct ccgaaatagt gcccgtccag ctggaatcgt
     4561 ctacgcggga gtgcagcacc atggcttcat ctttataaat cgactgtaca ttctgttcgc
     4621 tgatcgtgca cttcacacgg aattctccgt cggacataat cttcatcaac acatctgtgc
     4681 cgtccgactg aagctgttcg gtcgttttca gggactgcac cgttccggcg atctccgcag
     4741 tcactgtggc attgttaatg gtattctgta atttttccag ttcgatcttc ttggtcttga
     4801 tgtcgtattc cgacttggca atatcggttt gcagggactg gatctgcgtg gtgtaagtca
     4861 gcttatcgtc tgcactggca cttttctttt ccgtttccag ctggctgatc tgctgcttgt
     4921 tggactcgat ctcattctgc atgcgttcga tttccacgtt accctgctgc aattccagct
     4981 gcatggattc cacatcatag gtgaacagcg gatcaccggc gttgacgtgg tcgccctcct
     5041 gcacagcaat gctggaaact gtcttggagg tgtccagctt gacttccttg gtttcctgcg
     5101 gttctaccac accggaaaac tgcaagtcgg aaatcgttgt tgccgcagaa ttcacatctt
     5161 ttatcggcat cacgtatgcc acaccgtcca catcactgcc cctgctgtag tgccgccaca
     5221 ccagcacgcc gctgacgcat accgccgcag ctgtaacacc ggcaactgtg ccgattatga
     5281 ttttcttgga ttttcccata ttccttcctc ccagtccatg ctccgcagca gtgcgttctg
     5341 ccgacggagt tttttgaagc agtcctgcac aaatccaggg caataccgca caaagagcgt
     5401 ctggcgactt tgcacgccat ctgcttgcag agtttcccta gatgctgatt tcatcttttt
     5461 ggcagctgtt tcaactgttg tattcctcat gcaacggatt gcctttagta taccacacct
     5521 cttgacggtt gtcaagaggt ttttcaaaat ttctgcggaa gatttatttg ggaaatttgt
     5581 gcaaaggtga gaggcgggag agggttgtgc ggtgttcccc tagaacggag aaaaatcacc
     5641 ttacaaaatt cgcatatcat tatcgtatca ccctctcctg aacagatgct ctcacgccga
     5701 aatccgacaa taccgttccc cttccagcac ctgctgcata tacttttccg gtgttgtcat
     5761 gcagacagca ctctgaaacg tgaaaggact gcagccggac accagcactg caccggtatc
     5821 gtctttcttc accggaaact gccggtttct gctgcacacg ttccagtaga ccaccgcagg
     5881 cagctgatac cctgccgcct gatattcctg cttcatctgc tggaatatcg tcacgccgtt
     5941 gccggactgc ttatcgaatt ccatgtccga gatcacgtag atcactttcg gcagttcctc
     6001 cggcggcacg tggtgctgaa ctgcactctg aagtatcaga cgaaatactt tttccagatc
     6061 ggtattgctg acttcattat aggacataca gtgctccacc ttttgaaaaa tgtcctcccc
     6121 tttcagagca atcaagcggg ggttgtcgga aaaggtgata aagtggttgt ggaagtatcc
     6181 ctcatttcgt tcggcacagt aaatgcccag cgaaacggca acatgaatcg gcatcacatc
     6241 gccggaatag ttccagtaca tggaaccgga accgtctacc acggcaatgg cattttgttc
     6301 cggcatataa tccggcaggg atttccatgt cacgtccagt atccgctgtt tttgtgcaat
     6361 gtcatcataa aacattgcgt tttcctccaa ttcgatgcag tcccgaacga tctcataggg
     6421 gaaaagctga cctgctttca gcagtccctt ttcctgctct gcccgttcca gaaactcgga
     6481 gtaccgctgt ctgtcatgct tgcagaatgc tttctgatac ttgtacaagg ctctggaggg
     6541 cagtttggca taatcaaagg tgtaatcctt ccggcaaagc cgcttttcca gcacgtctgc
     6601 ataggtgcga agctgggaga gcattttccg gtaagccttt tccgaaagat gcagccgctt
     6661 acagagcagc tttgcctgcc ggcacacgtt ctgattggag gtgtgcacag agggcagcca
     6721 cttcgccagc agagagatcg gcttttcttc cagcatgtcc tgttggtctt gctgtagctg
     6781 cttctggatc agctgtatca gttccggctc acagggcgtg ttcagcaata ccatacaatc
     6841 gtcaaaccgc ccgtactccg gaatccactg caaatttttc cggacacttt cgggatagat
     6901 cgttgccgca tattgcagca tctgccggaa cacgctccgt tcacccaatc cgccgcgggt
     6961 atcccgcagg taaaacagga tccgcattgc gtataccgga ttctctgcat atgccgccaa
     7021 gaatatgtct ttcaggcgtt ttgcagaagc ttcccgcaac gcaccggctg tggaaaacag
     7081 gttcaggcac gcatcgccgg tatcgtgata cgccagtgct ccgttttcgg tgtttgtcca
     7141 gttcagttcc tctgataatg cattcagcat gaaaaacacg tcctttcttg ttatgtactg
     7201 tacgattgtt gaaaaagccc cacacgaaac cggtttcatg gcttcgtatg gggctgccta
     7261 aatgctccac gatgctgttt cgatggagaa cccatcttat tcaattttag gcaataccgc
     7321 acaaagagcg tctggcga
//
LOCUS       DAWBGR010000128         7223 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2713_length_7223_cov_4.785296, whole genome shotgun sequence.
ACCESSION   DAWBGR010000128 DAWBGR010000000
VERSION     DAWBGR010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2713_length_7223_cov_4.785296"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1766 a         1968 c         1785 g         1704 t
ORIGIN      
        1 tctcatctgt tcgtcccatg agataatcta cacttacccc ataaaaatct gcaagatgtt
       61 cccataccac tgtgggtata tcattttctc cagtttcgta tttggaataa ccggtctgtg
      121 acatctgtaa atactgtgcc acctgctttt gcgtcaaatc tgcatcttct cttaaatctc
      181 tgattcgatt tcgcataata ttccttttgc aatatttttt ctactgcttt ctttttggcg
      241 gataaggctt tttctcatct gttcgtccca tgagataatc tacacttacc ccataaaaat
      301 ctgcaagggc attcagcgat tccaaaggga tttctcttgt tcccagttca tatctggaat
      361 atgtcacctg cgagcagtgc aggtaatctg caatgtcttt ttgatataaa tccatatctt
      421 cccgtaaatc acgaagccgc tgtaataaca agttcttttc ctccgttttt tactcattat
      481 atgccaaacc aaaacggtat attgacttat aaaccaaatt ggtatataat aaagcaaaaa
      541 ggaggttttc ccatgacaaa ttacaaaaga ccttacttcc ggttattctg tgccgtctgt
      601 gacaccattg atgcactgga acaaacagcc gcttcgaaaa actgccccga aaccactgcc
      661 aaacttcttt cagaacaggc gaagcagttg cgaaaagtca cacaggaaac agaggaactg
      721 gtcattggca gcgattccta acgtttttac cggctctttt tttccgcaaa acgcttgtaa
      781 tttccgctga aatattgtat aatgaaagct ataggaatct gccctgcggg tttcaattta
      841 tcataaagga gtttttacca tgtacgcaaa tatgatggac accattggtt ttgtttccca
      901 gttcgatccg gaagtcggac aggcaatgaa tgaggagctg ggtcgccagc gtcggaatct
      961 ggaactgatc gccagcgaga acctggtttc tccggcagtt atggctgcta tgggttctgc
     1021 cctcaccaac aagtatgcag aaggtctgcc ccacaagcgg tactacggcg gctgcgaata
     1081 tgtagacgta gtagaggaga tcgctatcga acgtgcctgc aagctgttcg gtgccaagta
     1141 tgccaatgta cagccccatt ccggtgcaca ggcaaacact gctgtatact tcgcactgct
     1201 ccagccgggc gatacggtta tgggcatgag cctggcacac ggcggccacc tgacacacgg
     1261 ttctccggtc aacatctccg gcaagtactt caacttcgtt ccctatgaac tgggcgatga
     1321 tgaatatatc gactacgacc gtgccgagac tctggcaaag gaagtcaagc cgaagctgat
     1381 cgttgccggt gcaagtgcat atccccgcat catcgacttc aagcgtctgt ccgagatcgc
     1441 aaagtctgta ggagcatacc tcatggtaga catggctcac atcgccggtc tggttgccgc
     1501 aggcgttcac ccgtcgccgg taccctatgc cgacatcacc actaccacca cccacaagac
     1561 tctccgcggt ccgagaggcg gtctgatcct gaccaacgac gaggaactgg caaagaagat
     1621 caacaaggca gtattccccg gcacacaggg cggcccgctg atgcatgtga tcgcagcaaa
     1681 ggctgtctgc ttcggtgaag cactgaagcc ggaattccag acctacggca agactgttgt
     1741 tgcaaacgct tccgcactgg ctgacggctt gatgcagcgt ggattccgtc tggtatccgg
     1801 cggcacagac aaccacctga tgctggtaga tctgcgtcct ttcggcgacc tcaccggtaa
     1861 ggaattcgaa cgccgtctgg acgaagtgtt catcaccgtc aacaagaatg caatccccaa
     1921 cgacccgcag aagccgttcg ttaccagcgg tatccgtgta ggtacaccgg cagttactac
     1981 ccgcggtttg cagccggcag atatggaaat tattgcacag gcaatgtacc tcgccgcttc
     2041 cgacttcgag aacaaggcag aggaagttcg cgagagtgtc tgcgaactgt gcaagaagta
     2101 cccgctgtac gaataaacca tgcacacacc ccttgcagat cagattcgcc ccagcacgct
     2161 ggacgatgtg gtaggacagc ggcagctgct cgccgccgga aagcctctgc ggcgaattat
     2221 tgaaagcgga aaaatcccca acatgatctt ctatggtccc tccggcgtgg ggaaaaccac
     2281 cgttgcccgc atcattgcgg acagcacttc gatgacactg cacaagctga acggcacctc
     2341 tgcctcgacg gcggatattc gcgacgtggt ggcagaagtg ggcacctttt ccggcatgaa
     2401 cggcattctc ctctatctgg acgagatcca gtatttcaac aagaaacagc agcagctgct
     2461 tctggaatac atggaaaacg ggcagatcac cctgatcgcc tccaccacgg agaaccccta
     2521 ctttgccgtg tacaacgctc tcatcagccg ttccaccgtg tttgagttca agccggttcc
     2581 gccggaggaa ctggaacgtg ctgttgcccg tggtttccgc tgtatggaac aacagctggg
     2641 cgaaaaaatt cacctgtcag acggtgtctg cggctggatc gcacggggct gcggcggcga
     2701 tgtccgcaaa gccatgaaca cggtggaact gatcgtgctg tccggtgaac acaccgaaaa
     2761 cggcattgtc atttccgatg aactggcaca ggaactgacg caacgcagca acatgcgata
     2821 cgatcgggac aacgaccagc actatgatct gctgtctgcc ctgcaaaagt ccattcgcgg
     2881 ctcagacgaa aatgcagccc tgcactatac ggcaagactg ctggcggcaa atgatttgct
     2941 gtccgtctgc cggcggctgc tggtcattgc ggcagaggac gtggggctgg catatccgca
     3001 ggcaattgtc atcacaaagg catgcgtaga cagtgccctc cagctggggc tgcccgaagc
     3061 ccgtattccc attgcagaag ccgtggtgct cctcgccact tcgccaaagt ctaacagtgc
     3121 ctacgaagcg atcaatcggg caatggcaga cgtggaagaa tatggagcac cggattttcc
     3181 gcggcattta cagaataaac atttcgacgg ggcagaggca gctgtccgcg ggcagcacta
     3241 ccgctatccc cacgactatc ccaaccacta tgtcagacag cagtacctgc cggatattct
     3301 caaggacaag gtctattacc agttcggcga gaaccgacag gaacaggctg cggcagccta
     3361 tcgggaaaaa atcctgcggg aagatcagac gtaaagattt tacctttctc ctaaattcgg
     3421 cgggacagat gctccaacgc catgtcatac agacctgacg gcttgacttc acgccggatc
     3481 aggtcattgg caaagcggat cgtctgttcc agactgccgg aaaagtcggg aatgctctgg
     3541 cgttcttcgg tgtacaagtc ctccagcatg attccatagg tgggctgccg gatcccctgc
     3601 cgcacgatgc tgccggcagt gacacggtag accacacggt tgctgctgta gacttccata
     3661 taaacaatga gttccgcggg gcgggactgg gaacgggttt tcaacttcgt tttcattttg
     3721 tatgctcctg ttctggtaaa tagaatcttt ttctctgggc agtgctgcat tttgcagcag
     3781 atgttccttc tgtctaccag tataacattt ctccgcgaaa atgaaaaggc tgaaaagcca
     3841 cgtgttttgt cgaaattttg agaaagcaca aggaaaaaga caacatcgca caaggtcgac
     3901 agcaaagctt tttgcagcgt tttcccagac ttttccgcga agtgaaacga agcagacaca
     3961 ttgcaaccgc aatacgttaa aaacactgtg ctgtccatgt aaaatccaca agattcctgc
     4021 gattacagtc ggaaatcttt gtcggaaatg cgactagacg aacgcacatg atgttgctat
     4081 aatagagaaa catgcttttt cgaaagggga tctctgttat gacaaaagat atgactgcgg
     4141 ggaaaccgct ggggctgatc ttacgcttct gcggtcccat ggtatttggc aacattttgc
     4201 agcagttgta cagcatggta gatacggtca tcgtgggacg ctatctgggt gccagtgcct
     4261 tggcaggcgt gggtgccacc ggtgctatca gctttctggt ggtgggattt gccaccggca
     4321 tctgcacggg gctttccatt cccgtggcac aggcgttcgg tgccggcgac taccagaaca
     4381 tgcggaaaaa tattgctaac tcctattatt taggtgcact gatcggcgta cttctgacca
     4441 ttctcaccat gatcggcaca ggaacgatcc tgcactggat gaaaacgccg gacgatattt
     4501 atcagtattc ctacgattat atcattgtca ttttcggcgg cattctcacc tcggtgctat
     4561 acaacagcct tgcggcgatt ctccgtgccg tcggtgacag caagtccccg atcttgttcc
     4621 tcgccatttc gtctgttttg aatatcgttc tcgatctggt atttctggtg ggactgcact
     4681 ccggcgttgc cggtgccggc tatgccactg tcatttcaca gggtatctcc ggactgctct
     4741 gcctgtttta cctgtacaaa aaattcgaga tcatgcggtt tcagcaggga gaatgcagac
     4801 tggaactgaa tcggtgcagc cggctgctgt ccattggtct gccgatggca ctacagtttt
     4861 ccattacggc gatcggttcg atcattctgc aaacctctgt caactccctc ggctcgctgg
     4921 cagtcaccag tgtcactgcc gcacagaaga tctgtgccat tgtcatgggc ccgctggaat
     4981 cgcttggcat cacaatggca accttctgcg gacagaatct gggtgccaaa aagttttacc
     5041 ggatccgcga cggcattcgc ctgagcatgt acgtttccat gtgttactgt gcattctgca
     5101 ttgctttcct gtggattctg ggaaaataca ccgcatacct gttcctcgat ccctctgaaa
     5161 cagaagtcat tcagctggca gtacagtaca tgcgtctgaa cgcgatcttc tatccggtgc
     5221 tgggcgtgct ctttatcctg cggaattccc tgcagggcat gggctacagc ctgatgccga
     5281 tgatggcagg tgtcagcgaa ctggcggcac ggtcgctggt ctgcttcggc tttgttccca
     5341 cctttggcta catggctgcc gtcatggcaa gtccggtggc gtggatcttc gccgacgtac
     5401 tgctgatcac cgtttacttt gtggacatgc gaaagctgaa acggcagctg ctccctgcgg
     5461 aggctgcggt tccggcgtga gaacattaag gaaacacacc ccaagaaagg atacttttat
     5521 gaaaaaattc atctcactga tcagtgcagc ggtgttctct gcaacactgg tgctgccgca
     5581 gacgctggct ttttccgctg cggcagaaga atctacactg gaagaaagca ctgtctttga
     5641 tgcagcggca gccgaagcca acccgtttca ggcaaccggc accgccaaca ccctgcccta
     5701 tcagctgcgt ttcgtaccgg aacggaattt cgtcacggca gaagaagttg ccgccggcga
     5761 cgtggtcatt ccggcagctg tgtatatctc cggcagtact gccaaccgct tcggctccgt
     5821 tatggtaaag tacgatgcgt tccgcaacga tgaaaaaact gacagcgttt acttctccgg
     5881 catctccacc ggcgacaccg ctcaccggac ttcggaaaag aaaacctata acagcagtca
     5941 gggcacgttc actacggact atgtgccgta ctgcttcggc tctgtttatg atgacgaata
     6001 cgacagcaat tctccgatgt tcaccaccag agaatatgcc tgtgacccga tcttcggctc
     6061 gacgatcacc agtgccggaa agaacaagat caagttctcc gcttcgttct acaacagtgc
     6121 agacgaacgc aaaaacggcg tgaaaaagac caccaaggaa ttcgttgcag atgtgactgt
     6181 agacgagaat ggcaacggtg tgtactccta cgactacatt gaccagagtt cgttccagac
     6241 cattaccaag acggcaacac tcccccgcta caatccggac acaccggaag gcgaaaagct
     6301 gccggatacc tgcaacagcg tcatgtgggt cgctggtacc agccagctga aggacggtgc
     6361 cgaattcctc ggcagcactt ctgacgaatt cccgctgttc catgtcaata tcgttatctc
     6421 caagggcact gcttccggca tatatgacgt gcagttcctg acacgggaaa ccgtcggtgc
     6481 caacgaggct cccacccagc ttaccagcaa caagagcaag gaattcccca ctgaactggt
     6541 gggcacctcc attgcagtag gcgtggacag cgttgctgtt accgattccc agcaggataa
     6601 cgtacagttc tacacggaca gcaacaccaa catcattcgt gcgacagact ttgcaagcaa
     6661 gatccttgca gacgtgacct ataccgacgg caccacagaa tctgatgtgg acatcacccg
     6721 tctggtggac tgctacggtg caacaccgaa ggaactctat gacaaacagg caaagaacgg
     6781 tttcttctcc tctcccaaca tgcccctgta ctataacggc aaactgctga agtggaagac
     6841 taccgacaag aacgtgacac agaacatcat ggtaggcaaa cgcggcgacc tgaactttga
     6901 cggcggcgta gactccactg atattttcta cctgtccctc tacattgccc aaaaggctgt
     6961 cggtatgaaa accactctgt ataccggtac ttccaccgat gccaacatgg aggattttgc
     7021 attctacctc atggactttg acacgctggg acagaccagc acggcaactc tggacagtac
     7081 agacatttat tatctcctcc tgtacgttgc aaaccgtgca gtaggcaacc acatcgcctg
     7141 ggattctttc gtcctgtaag ctgatccttt tataaggcaa taccgcacaa agagcgtctg
     7201 gcgactttgc acgccatctg ctt
//
LOCUS       DAWBGR010000129         7208 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2721_length_7208_cov_4.052006, whole genome shotgun sequence.
ACCESSION   DAWBGR010000129 DAWBGR010000000
VERSION     DAWBGR010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2721_length_7208_cov_4.052006"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1575 a         1850 c         1702 g         2081 t
ORIGIN      
        1 tctgcacgca gattgcgtgc aaagccgtca ggcgggcttt attcagtgtt tcctttggta
       61 tatgatgtta tcttagatca tgtcaatgtc ttttagttgt tcatatgaca gaatatccca
      121 gtgtgccaga atgttgcggg cattccgcat ctttttcagc atttcaaatt ctgcatagtc
      181 tgtaatcctg ctgccgctgt tctctttgca gatgtagaac agctgcccga tctccagatc
      241 ggctgcctga tctaccgtat cattgttgga actgcgaatc ggcagatact gctgcagttt
      301 ggacgcatac tttcgaatga tctccacacg gaaattttcc agcttcggaa ataccagctt
      361 gatctgtgcc tcccagacgg ctgcacgcac gacgttctgc aagtttgtga ccttgatgtc
      421 attttcctga tacacgcgtt ctgtcacgcc aaccggatcc cgcagcaagt ccagattttc
      481 cgctgccagc agtgcggcac gctcaatgtg gttatgggca atgttgcttg ccacttcaca
      541 gagatacatc ttttcggctc ttccgcagga cagatcggat accatagtca ggcagagcat
      601 catgcagtca tagtccgtga cataatcggt ataccggaat gcggtcagct gcttggttcc
      661 caccgccggt gctccctcta tcattagcag gaaaacgccg tgttcctttg tcggatcaca
      721 gttttccata tactctgaaa tggaagcgat ccattttgcc gcgtgttccg gtcggatacc
      781 agtcaggcag acatcttgct tattcagatt ggtgtcgcgg ctctgtgcca gaaaacgcat
      841 ttcattgccg tgcgtggttg gccagtaaac attcttctcc tcttttctgc aaaattcttc
      901 caacaggtaa gatcctattt cattactatc cgatacacta gatacattca acactctcag
      961 cttgcggtcg tcgttctgat ctgccagccg ctgttcaatg gtttcgatca tgatgtcctg
     1021 ccacggaatc tcgtcatcga aaaacaagac taccgatttt tcgttcatga ccgcaatctg
     1081 aatgtcatcg agaaaccgca cggaattgac aaggcggttc caccagagtt cccctatcat
     1141 tcggatacct cctcctcaaa atagtctgcc atgctctttt ccaccttgtc ctgactgcca
     1201 aggaatttcc ggaatccgtc ggtggcgaat cgataataat tatccatgac actgacaacg
     1261 ttcagatcca gcatttcgtt caggatttct tcgaactggt tgggtttcag cgaagtgata
     1321 cgggtgatgt gatattcttc cgcgatccgc agcagatcat ccctggtata gcccttttca
     1381 ttcggcgaaa cgtagcacag atacgcaatg atcaatgcaa tgatgtgata attgctccgt
     1441 tccttggcat cggtaaacag tgtggcttct agcttctgat cgactttttc agtgaagtcg
     1501 gtatccgaga ggactttttt atagtggctc tcagtcactt cataaaacgg tgtgacagat
     1561 tctccgtagc cggcatagtc ctcgttcttc attgcctcca gaagcttctg gcagtagaac
     1621 tggatcagtc ccgggaagtt ataggtagaa gcaagaatgt tgtcgataat gtcctgattg
     1681 aaccgaaatc ccagatagga aagcgtacag gtcagcagct tggtggcttc ttcccgccgg
     1741 aactgcttga tgacgatcca gttcaggtgg atcaggttgg agttttcgtg catcatttct
     1801 ctgtgatagc ggcttacatt gtgcagaccc gccatgacta atttgaagcg gccggagggc
     1861 aggctcttga gggcagtgat cggagaatcc tctgtgtcac cgctggtctt gatgatctcg
     1921 tccgcctcgt ccagcatcag cagcagatag tggatccgtg tttccggatt ttcgtccatg
     1981 aaccgcttct ggagatgtcc ggcgagggca gtccatgtgt cacagcgggt ggattcgtcc
     2041 agtatcccct ccagaatcag cttgtcagac acgatcttga cggcttctgc tgcactgcgg
     2101 tctttgagtt cttccaccag aacagctcta tcgccgttgc cgtttttatc gatgttgtgc
     2161 cgtgccattt tcagcagggc actcttgccc agctggcggc cgccgtaaac cagatttgcc
     2221 ccttccggcg actcgatctg ggtcagttcc gcttctctgc cggtaaacag ttccggcggc
     2281 atgtcctgtg tagaggattc tacgaaaggc tgatagtacg caaagggcag cgttactgcc
     2341 atgagccgcc gaatcacggt attttccgca tagtgctttg ccagatagaa caaaatgaca
     2401 cggtcgatca cgataaacgt ggtggcgaaa gatttttctt ccttgatctt tctggcaagc
     2461 cgtctgcgtt cttccatgtt catggcgaag tccagcagca caagggtatg ctttgccacc
     2521 gcattgacct cgcggaactt atccatcaga ctgtcacagt cgtatttgcc gtacagacac
     2581 agcacgcgga agccgtctgt ttcgctcttg gagccgaacg ccggtatcgg gtgaacgtag
     2641 ttgatcttac cgatctgttt ccggcagaac acctgatatg cttctgtgtc cagactggtg
     2701 tccggctgaa cggaaaccgg cttaaatccc agataagaga gcagtttttc cagctggttc
     2761 agactggcag gaccgccgga tggaatccag cttttcagca ggtttgcacc gcctctggtt
     2821 tccttacggg cattgtttgt cagatgcagc agtgccagat ccagatcttt tttccccgaa
     2881 tattcggcaa tcgtttcagt aatgtgcttt ccgcccctgc ggactgcacg gtagttggtg
     2941 gcatgctcat tgataaactc gcggaaatat ccaaacggct catcggaata gtcagagatc
     3001 ccgctgacat cgcggcggag gatacagctc atgatgaact ccgcggaaga atagttctgg
     3061 tcattgatct tggcttcaat catttccttg ctgaacacgc caaagtcata gtccggactg
     3121 tctgccagtt cttccagctg gtgcagaagg cgttcgcctt tttgcacggc attgacagaa
     3181 atgcggttgc ggatcacttc cagcagtctg acatagaagc cgtaatcgtt ggtgacacgg
     3241 gtgatgcggt accacgcgta ggcaaccgcc atgatcgcac tcttttcccc gttgatgttc
     3301 gaaagggtgc cgtagctttc acagagttcc agttcatcgg agaaatcctg aaacatggtt
     3361 tcgaaccgct gctttgcctg cttcaggcat tccccgtact gggcaaggtc tttgtgttcc
     3421 ctgatcgctg caatgtccat gtcctctcca taggctttga tgagccttgc agtacggaaa
     3481 ttgtgcttgg tttccacatt gctgagaatt tcggagagcc gttccggcag ttccggatgc
     3541 ggcttggagg catgctgttc gatgcggtac agaatgtgga aatccggcat gccgcagaac
     3601 gtggactggg tttccggcag gtagttgtcg ttcagcagaa cgtcttcgcc ccgcaggaag
     3661 tccatgaaga aatactttct ggttttggtg ttgtatgtgc cgttcatctt ttccagcagt
     3721 tcggtggctg cacggcgaac gctctcggtg ccccagtcga atccccgctc tgcaagcatg
     3781 tcattgcaga tgcgaacgat ctctgtcagt tcctctgtca ccttcggctc cagcgtgtca
     3841 tactccttgg caaaagcatt gtcggtactg ctgaaatgct ctgccacagc gagccagtca
     3901 cagatgcagg aaagcattcg cttcagcgtt gccataatgc tgttccgctt gctgcccttg
     3961 attttttcat gaggtctggc gatgtgcttt ccctcatttt ggatcgcaac gcgggcacgt
     4021 tcccagaaat catcaatgca cttatctgct tttttcacgt ccagatattc catctgtata
     4081 ggcttattgg cacgaatgaa caattccgac accgtatcct tgacaaactg gaacttgtcg
     4141 gtttcatttg ctgcaacgat gttcagacag gtacggagtt cactttcctc gctggcgaac
     4201 agcagttccc gtgtacagcg gacttgtccg tggttctcat acacatcatt ccgcatatcc
     4261 gccgcattac agcactcctt cgcctcatca atgaccgatt caatgacact gctgttggtc
     4321 ttataggcag caaacgagtc catgccgtat ccggtttttt cacggaacga taccagatcg
     4381 cccagcaggg gcagcattgc cggataggta tccagtccga aattttccac cacgaaagaa
     4441 aagtcttcaa tgctgtaatc cggtacagaa ggcgtatgga acagggcatg catggcagaa
     4501 gctgcaaaaa gtgatgcact gatgtccggc aggacgttct gtgcctcatc aaatactgcc
     4561 atgatctctg tgctgagata ctcagagtcc agaagcggac tgtgaaacgc ataggcaaat
     4621 gctgcatctg ccgccctgat cgcctgtccc acatagacag tggtgccgtc ctctgtgttc
     4681 cgcacgattt cggaatcctt tgtcagcatt gccgcactgt tgagatatgt gagcaggcag
     4741 tgcagttgtt ccggctggaa gtgcagagcc ttgctgataa ccgtactgtc atcaattccc
     4801 agagcttcga agccggcggt atccgtgtag gattcatacg ctgtttgcag agattcttcc
     4861 ggctcagagc cttcttcgaa gccatcatct gcatcgttgt ctgcatccat ggcattcgtc
     4921 tgtctttcgg cttcttccaa tgccgccttt ctgtccgcaa gattttgcag cattacccgt
     4981 ttccgttcgc agtcgctgga gagcccgaca tatttttccg cattctcata gtcaccggtt
     5041 tcaaaataca gttctgccag ttcgatataa atacaagtgt tccgtatcgt gtagcgttct
     5101 ttaattttat ttcgcttgac aatatccagc cagtccagca gttcgctgat cgcggaagga
     5161 tagtctttga tttcccgata acaacgtccc ttcccctgca agtaatgcag gatcttgctg
     5221 tgctcgccgg ggtcgcactg ttccatcagg cgattcagga tccggatcgt ttctgcatat
     5281 tggtgtgttt tcatataata ctgctgaagt gcttccagtg cttttaacgc ctttacttcc
     5341 cgatccgcat atttttccac aaacacacga agttcgttca gataccccaa ttcttcattt
     5401 tcattgttca tacgtaaata acacatgatg atcgctgtaa aagcgtcttc ccagcaatct
     5461 ttatccatat aagtcttgca gacctcgaac accttggaat acgctttttt tgccgtccaa
     5521 tggcttatgg cggcactcag ctgctttccg tcctcacttt tttccaaggg ttcttcagaa
     5581 gcaggtgcag atgtttcctt ctctgccgct gctgccttga aaatagaagc tgacaggggc
     5641 agatctgctg atgctgcggc tggcgtttct gccgcagctt caactgcggc attccactgt
     5701 gctgacggca agtaaaacga tgctgttccg cgtttaatgg ataccgccag ataatttttg
     5761 ccgcttttca gcagctggaa ctttaccgga ataggagtca ttgttctttt gttgatcttc
     5821 ttaatatttt tttgaagcga tttgtctgta atgtccttca catcaaacag gtactgttct
     5881 tcgttggggc cctgaatttt tcccttttcc tcgaaataat cgtaagaaat gatatagccg
     5941 gtgtgtgtct tggtgagatc cggctcatta tttgcaggcg gtgtttccgg catgtccaca
     6001 gattccggtt tggtttccaa gccggatttc agagagttgc tctgcaattt ggtgattttt
     6061 ttccgcaccg cattccccgg gaacagatac cgcagacttt ccagcagtgc ctgcatatcc
     6121 tgtagttctg ccgtggtctt tccatgatct acgccaatgg aacgaatgat gcggttcaaa
     6181 gctgccctgc tgctttcttc cacagcatgt tctaatgcat aggtgatacc ggagatgtct
     6241 gccaaccgta cagaggactt ttgcagcacc tctgcggctt cttctttgcc cagtgcatta
     6301 tcctcagaaa gaaacaacgc agcaaaggcg gcagcaagga gagcgtattc ccgttcttct
     6361 tttttctgtg ccccctgata tgccgcacag taggcatacc gcagattgcc ggcatagaac
     6421 agcgattctg ctgccatatc atagtcttca cagaccagct gtaacaagcc cagaaaaaaa
     6481 ttgacacgag gattataatc ccactcattt tctttgatca cattccacgc cagctgaact
     6541 gcctcctgac acttcgaagt ttcatgactt tgcagataat tcacatattt attatatcct
     6601 gttgtcagag ctttcttctc ttgggattcc atagctttaa atgcctgtgt tacagcttct
     6661 ttatcgcatg gaatctgcat gttttcaaaa ccagatactg gtttttgttt tggagccgct
     6721 ggtgccggaa ccgctgccgg ctgtaccggt gctgcttgca aaacaggatc agctgctggt
     6781 tttggctgtg gcatgccggc ttgtgtacat tcctccagta tgccgatctg atcgtaccgc
     6841 agcgtggctg ttgatgtgat ctgtaccgaa agagcgtctt ttccgtcatt ttccatgaca
     6901 atgccctcaa taatctgtcc ggatctgtgt acaagtcgaa catgactgcc tatgggcagt
     6961 gccgctagtt tttggtctaa taaactcatt ggttgtacct cctgtaggta tgccggtata
     7021 aattttgttt ttgccggcat gatggaaata ctatcctata gatagcatag tattattata
     7081 catctgtttt gcccaaattg caacatagaa ttgtaaaact tgatgctttt tcacatttta
     7141 tccatgacgc aacaattctt tggaaaataa aggcgacacc gcacaaagct tgtgcgtaag
     7201 gtgcgttg
//
LOCUS       DAWBGR010000130         7193 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2728_length_7193_cov_5.792799, whole genome shotgun sequence.
ACCESSION   DAWBGR010000130 DAWBGR010000000
VERSION     DAWBGR010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7193)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2728_length_7193_cov_5.792799"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1503 a         1959 c         1895 g         1836 t
ORIGIN      
        1 tatgacggaa aatctgcacg cagattgcgt gcaaagccgt caggcgggct ttgtgtggtg
       61 ttgccttagg aaaatcgtcg tatttccgct ggacaacccg ccggaaatgt gctatactaa
      121 aagtattccc aaaccgttcg tttccggaac ggaaattcaa aaagtcagga acgtgatgta
      181 aatgaagcga tttcagcaga atatccggaa agtggtgccg tatgtgccgg gagatcagcc
      241 ggattttccc gatatgatca aactgaatac caacgagaac ccctacccgc cggctccgga
      301 agtgcaggag gttctggcaa agagtaacgc tgaacagatg cggctctatc cggaccccac
      361 cgcacaccag ctgaatgctg ccattgccga ggcatacggc gtagaggaag cacaggtatt
      421 caccggcgtg ggctcggacg acgtgctggc aatgatcttc cagacctttt tcaacagcga
      481 tctgccgatt ctgtttccgg atattacata cgcgttttat ccggtgtggt gcgacctgtt
      541 ccggatcccc tacgaggtgc agcccctgaa cgagaagctg gagatcgtgc cggaggacta
      601 ctacaagccc aacggcggca ttgtgttccc gaaccccaac gcccccaccg gcatcgctct
      661 gccctctgcg gcaattgagg agatcttgca gcacaatccg gagtcgctgg tggtcgttga
      721 cgaagcctac gtggactttg gcggggaaac cgttttgccg ctgctgaaaa agtatgaaaa
      781 tctgattatg gtgcagacgt tctccaagtc ccgtgctatg gctggcgaac gaatcggctt
      841 tgccattggc tctgtggaac tgatcaaggc tctgcatgat gtgaagtatt cgttcaactc
      901 ctacaccatg aaccgttctg cactggatgc cggaaccgca gctgtgcaga atgccgcata
      961 cttgcaggag accaccgcca ggatcgtaaa gaccagagaa cacaccaagc aggtgctggc
     1021 ggaaatgggc ttccggtttc cggactccaa gagcaacttc ctgtttgtga cacatccgga
     1081 ctgcgatgca catgaactgt tcgaggcact gcgggaggca catattttcg tccgctggtt
     1141 tgattctccc agaatccgga actatctgcg gatcacgatc ggcaccgatg ctcaaatgga
     1201 atcgctgttc gcattcctgc gggcgtatct gaaaaagtaa tttctgttgg acaaacatca
     1261 aaaaagggga agcttcccgt gagaagcttc ccctttttca ataggagata cattttatga
     1321 aactgcggcg taattaccgc aggggagaac ctgtgaatgg acagatccca gcaggtttac
     1381 tggatctcga tctgttttac ttccggcttt gccggctgct ttttcggcag tgtcagggtc
     1441 agcacgccgt tttcataggc ggcatcaatg tgatccacat cgacattgct gatgtccagc
     1501 cgctgcaaat acttgcagaa gctgcgttcc cgctgtaaat aggtgccctt gctttcgggc
     1561 tgttctgccg tttttgtttc gccggattcg ccgtctttct cgggggcttc ttcgttctgt
     1621 ttctctgcgg tatgctctgc cgcaattgtc agaacatcgc cctccaagga cagcttgatt
     1681 tcctctttcc ggaaccccgg catgtcggct tccagcacat agctgtcacc ggtatcccgc
     1741 aggtcggttt tcggcacttt tgtgatctct gtgtctttca gagaattcca cagtgtgttt
     1801 gcaaacggtt gaaaaatggt catcataagt cattcctcct tggaattatc tccagcagtc
     1861 catgctgctg ttctttcttt gttctgtatg ctgtgaaaac agcacttttt ctctctatct
     1921 atttgtcgtg ctctctttgt gcggtgttgc cttagtggat ttccagctga cgggaaaccg
     1981 gagcagccgg ctgctttttc ggcatcgtca gtgtcagcac gccgttttca taaactgcat
     2041 ggagtttgga agtgtccaca ttgctgatgt ccagcttctg ctcataggag ctggtgctgc
     2101 gttcccgacg gagataagtg ccgtcttttg ccttttcctc ctcggtcttt tccttgtgga
     2161 cggcggacag tgtcagagtg tcgctctcta cgctgatctt gatctcgtcc ttctggaatc
     2221 ccggcagctc tgcttccagc acgtagctgt caccggtgtc ccgaatgtcg gtcttgcagg
     2281 aactcatcgc tgtggacttt tcgccgaaga attcattctc ccagtcccgc atggaacgga
     2341 acaggtcata accggtacgt tcaaaaggtg tcaaatatgt catcataaag cattcctcct
     2401 atgcatggtg ttctgatttt gtggtttgcg gaacggtgct gaccggtttg ctgtctgctt
     2461 cttccggagc tcctttccgt ttacaagtat catattaccc ccgctgtgtg aaagttcaag
     2521 gttggttctg tgaaaactag atgaaaatta gcactcttag caaaagagtg ctaacaagtg
     2581 ctaacggtga gtgctaactc tgcaaatgag tatcgaaaag aaaaacgggc acagcatgct
     2641 gtacccgttt gcaatatttg tttccaaccg atccggcttt tcaatgtgag atcacttaaa
     2701 ctgatatacg gcgatatttt caaagccgtc cagcagggtg tccattttgc ggaatgcaat
     2761 ggcagtgccc tttgccacgc agtgaatggc atctttggcg actacgcact tgacctgcaa
     2821 agttcgttct accagtttcc gcagaccgcc cagcatcgca ccgccgccag ccatgagcac
     2881 accgttttcg tagatgtcgc ccaccagttc cggcggcgtt tcttccagaa ccgtcttgat
     2941 cgcttcgatg atgtcggcga tcacatcttc cacggcttca aaaatttcca gttcgctgat
     3001 ctcctgcatc tccggcagac cgctgaccag attccgtccc ttgacccatg atgtcacatc
     3061 ggctctgggg tcgtacaggt tcgtcatttc gattttcagc cgttccgcag tgcgttcgcc
     3121 gatcagcagc ttgtacttgg tctgcatgta gcggataatt gcctgatcca gcgtgtttcc
     3181 cgccgcacgg acggagtgag aggtgacgat gccgttcatg gaaacgattg ccacgtcagt
     3241 ggtgccgccg ccgatgtcca cgaccatgtt gccccgtgcc ttgccgatgt tcacgccggc
     3301 accgatcagg gcggcaatgg gctcatcgat caggtatgcc tttcgggagc cggcactcat
     3361 ggcagcttcc acgacggcac gcttttccac gtctgtgata aaggacggca cacagatgat
     3421 gatgcggggc ttggtgagcc ggtggccggt gacctgtaaa atgaattcgc ggatcatgta
     3481 ctgtgtcatg cggtcgtccg aaatgacgcc gccggacagc gggcgaatga cctcaatata
     3541 atccggtgtt tttccgatca tctgataggc acgcttgccc actgcaagta cttctttggt
     3601 gtgggtgtga taggcaatgg cagagggctc gctaagcacg acaccctttt ttcctaaggt
     3661 gatgaccgtg tttgccgttc ctaagtcgat accaatatct gttttgctca tagcgattcc
     3721 tcctgtttct gggtgtcctt gatttctccg tccggttcgt cagactggga aatcggaagt
     3781 gtctgcgtgg gcttctgctg ttccagcata tcccgcagac aggtatatcg attcatacgg
     3841 cggttgttgt ccaccatttg ataaatgacg ttttgagagc cgatcaggat cagcagccgt
     3901 ctggctcgtg tgacggctgt gtacagcaga ttccggtagt acagcttcgg gaatccgccc
     3961 agcagaggca tgacgaccgc ctgaaattcg ctgccctgac ttttatgtac ggtgacagca
     4021 taggcaagtt ccagctgatc tagctgttca aagggatagg tggcacgtct gccgtcaaat
     4081 tctgcaatga cttcctgacc ttgccggttg atcgcacgga gatacccgat gtccccgttg
     4141 aaaatgccgg tgccgttttc gccgtccttg tgccagatga tgtcgtagtt gttcttgatc
     4201 tgcatcacct tgtcgccctc ccgaaaggtg tacaggatcg aacggcattc cggcttgtct
     4261 acggatttgg ggttgagtac cgcctgtagc tgctggttca gttcaatgac acccatgctg
     4321 cccttgcggg acggggtgat gacctgaatg tcctccatgg gggagtagcc ataggcgttg
     4381 ggcagtctgg ttttcaccaa gtccagcatc agctgtgttg cgtccttttc ctgtaaccgc
     4441 tggaagaaga aacagtcgct gtccttgcgg gacaggtcgg ggtgttcgcc ctgaatgatt
     4501 ttgtgggcgt tggtgatgat gctgcttttc tgtgcctgcc ggaaaatttc tttcagagcc
     4561 accaccggca ctctgtcgct gtggatcaga tcccgcagca gattgccggc tcccacagag
     4621 ggcagctggt cgcagtcgcc caccagaacg accttgcagc ttagccgcaa ggcacggagc
     4681 aggtgctcaa atagcagcac atctaccatg gacatttcgt ccacgatcat tacatcgcag
     4741 tccagcgggt tgtgttcgtt gtgctggaac cgcatgttgc cggacatgtc gtattccacc
     4801 gccagcatgc ggtgaatggt gcttgcctcg tagccggtga gatcagaaat gcggctggct
     4861 gcccgaccgg tgggggcggc gatcatcacg cggtagccct gcttttcgta cagttcgata
     4921 atggcattca gtgtggtagt tttaccggtg ccggggccgc cggtgaggat catgatgctg
     4981 cgggaaaggg cagtgacaat ggcttttcgc tgcaacgccg catactgcat gccctgttcc
     5041 tgctgctgtg tatcgatcat tttttcgaag atctcctgat cttccggtgc actgaagtcc
     5101 tgtatcacac cgatgcggtc ggcgatatag cgttccgcaa tgtagtagtc ttccagatag
     5161 acaaaatccc gtccgttctt ttcatagacg tagagggtgt ggtcttccaa tgctgccgca
     5221 taggcaattt caaattccgc ctcgttgacc tgcaaatact tgcacgccgt aggacggagc
     5281 cggtccagcg gcaggcaggc gttgccgccg ttggtgttgt actgtaagat gtagcagatg
     5341 cccgcctgta tccgctgggg agcattgtgg gcaaactgca tgccctgagc cacggcatcg
     5401 gctttctgga agtccagttc cacgccggag ctgcaaagca gataggggtt ggcggcaagc
     5461 atgtccatgg caccggtgcc ccagtgctga taggcacgca tggcatattt ggaacgaatg
     5521 tcatactgcg acaaaaacag catcagtgtc cgcagggaaa agatctgtcg gacttctgcg
     5581 gcgatcgctt cgcacttttt cggggagatg ccccgaatct ccagaagacg ggtgggctcc
     5641 ttttccatga tctccagcgt tctgtccccg aagttttcca cgattttttt cgccagcgat
     5701 ggtccgatgc ctttgatcgc accggaggaa aggtatcgct ggatattgaa ggcatctgcc
     5761 ggcagcttgc gttcccagta ctgtgcccga aactgcatgc cgaaacgggg gtgcgtggtg
     5821 tattcaccgt ctaccgacag ccgttcgcct tcttcggttt ctcccatttc gcccaccaca
     5881 gtgaccagtg tgccgtccgc gtccagttcc agtacggtat agccgttatc cggattgcag
     5941 tacagcacgt tttccacagt gccttccagg tgcagcgttt ttctttcgtt tggtgtcact
     6001 gcgggttcct cctttgacag cggcagcagg cgttctgcat tgcttctaca gctgccggaa
     6061 tttggctctc ctgtgggcgg gagagtacac ttcttttatt atacctgttt tttcgacaaa
     6121 ttgcaatgac ttttttcaca atctgtgccg cttccaaaaa ttttcgtcaa ctccgacaga
     6181 cggcaatagc aaaagtcctt gtgccggctg caaaacgctg tacatgccgg ttccagcgag
     6241 ccgtccggtg tcgcgtccac caaccagact gtccgcacca tgtcgcggtc cgtccacgtc
     6301 agctgtgccg ccagtgctgc acattgtgtt tcccactgct gtggattttc cagcagggca
     6361 agggtcgcca tatgtcgggg cagctcatgg gaaaacagcc gccggcacag tcccagtgct
     6421 gccagtgccg tgatcgccag cagcccgaac accagcaggc aaaccaatcc gttcatctgt
     6481 gacacctctc tttcagggaa cggtaaatat ccccaccgtc ggcgggaaca gactgaagat
     6541 cccgaagcag acggcaacgc ctgcaaacag caggatcccc aaccatgaac cgcctttggc
     6601 gtggggcgtg tgtttccatg tccagaatgc cgtcactgcc gcacccagca caaatgtgag
     6661 aatatctgcc cagagccagt gcctgccgca gatgccggta taggtgtaaa agagggcagt
     6721 aatggtcagc atgcccagca gtgctccctc tgcccgcgtc aggacgtagc ccttccagcg
     6781 gtgtcccacc cagatgtact cccagacggt gtagaccagc accggaaaga acaacagttt
     6841 cagatgttcc cacggacttt cgttgatcgc ggcatacagc cccacgaccc gattttctcc
     6901 tgtccagccg tacaaaaaat ggagcactgt cccaaagaca atgctccata gaaatccgat
     6961 ccagacactt ctgcggaatt cctgcatcac gcatcacctc aataccaagg tatgcagcat
     7021 gccggaatct ggtgcatgag agtctgtttc gcatcttcct acacgcatct tgcgtgcata
     7081 ttttccgaga tatttcggga aaatactcga aaatccacaa aggattttct ccgtttttcc
     7141 cttcatctct caaaaaatct gacgacgcaa tctgcatgcg tcagatacga aac
//
LOCUS       DAWBGR010000131         7027 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2788_length_7027_cov_4.328313, whole genome shotgun sequence.
ACCESSION   DAWBGR010000131 DAWBGR010000000
VERSION     DAWBGR010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2788_length_7027_cov_4.328313"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1681 a         1744 c         1869 g         1733 t
ORIGIN      
        1 tgctttcgca cggcaaggat ttttaacgac gaacggggaa aaatcacccc aaaagacacg
       61 tgtcaggctt agtgtcaaca caccctaatg tatatgaatg taggaacccc gattatgtct
      121 gtaggaggaa tccatgaagc agtatactgc ggataaacat gcacaatata cgctgcaaat
      181 tgcaacaccg atcgtgacgg cggtgctgat cggtttggta tggtactttc tggcggtgct
      241 gccccattgg ctgctgtgga cactgacggc agtgctggcg gcggcggcga tcctgctgtc
      301 cacactgctg ctgcctatgt ggttctgcac tgtgacctat tgtgtatcgg cgactcatat
      361 cacccgaaaa tgcggcatct ttttcataca ggagcaagtc atgcggacac aggcgttgca
      421 gttcagcagc atttttcagg caccctttgc cgatgccacc ggcatgaatt ttatcccgct
      481 gcatgcctat ggcggcacgg tcattctgtc gtttttgagc agttctgacg cggcggagat
      541 acaggcgttt ctgcaaaaga cggtctacag ccacagccag cggacactgg aagtgcctgt
      601 cgccggaaat cccgcagaca ttccgcagga gccgcagttc gatgcgtttg aaagtgcggt
      661 gcagccgccg tcaggaactg gcagcaatgc ggaagatccg ccggaaagga gcagcccatg
      721 atgcggcttg ggactgccgg tgaggcagtg tatcgggaac acccgatcaa gattttacgc
      781 tacagtgcca agaacctgtg gctgttgatt ttcccgctgc tgcgaagcct gcggttctat
      841 ccgttctcac ttcagaaact tatcgactgg ggagccggtg cgtggtttga cctgctggtt
      901 gccctgctga ttctgggcat cggtacgctt cgctggcatg cctgcacata ttatttcgac
      961 gaagtttcca ttcgttccca aagcgggatc ctcctgcggc gggggacgga gatcccgctg
     1021 gaacgtattg cctccacgat ggaggaacac ccgttttacc tccgccctct gcgggcagcc
     1081 tgtttgcaga tcagcaccgc agccggtgct gtgccggaag cggatatgca cctgaccttg
     1141 tatctgcggg atctgcaccg gctgcggcag catattcccg tgctgcaaaa tggcagcacc
     1201 ggtgcggtgg cgtatcacac gccggcgtgg cgaatgctgt tgttctcggc gttgttctcc
     1261 agcagctttt ccggtgcgat atacattgcc accatctgct ttcagggcgg acgcatcact
     1321 tccgatctgg taaagcagtt tcaggcacag caaattctgg aagatgccac cgacaaagca
     1381 tcgacagcgt tccacggcgt gccgcgaatc gccattacga tcggcattgt gattctggcg
     1441 ttgtggctga tctcgttcgg acggaacctg ctgcggtacg gcagattccg catgcggttc
     1501 ggggaggagt tcatctcggt gcatacggga atcctcaccc gccggcggta tcatctgcgg
     1561 gacaacgcca ttattttccc cgacctgcgg cagaatctgc tgatgaagat attcggcatg
     1621 gtatccctcc acattcgctg ccccggctac ggcagccggc gggatacgct gccggtgctg
     1681 attccgctga ttcggaaaaa aggctcacag gcactattgg aaaagctgca cacggtgccg
     1741 gtcatggagc acccgaaact tcatgcacgc tccaatatcc ggtttttctg gtcctttgtg
     1801 tggccgccgg tgatcgggct ttgtgcgatt ctgccggcac ggtttattct gctgtggctg
     1861 ctgccgaatc tgggggcgat cattcggttc tgttccgtga tgctgattat tccgctggtg
     1921 tggctgctgt gtatccgcat tgtggcgatg ttcacagaat cggtcaccat ggacgaccag
     1981 catttgcaga tgcatttttg cagttggttc acgttccaca ccattacggt gaaccacgcc
     2041 cgcattgtcc gcacggacct gatgcagaca cccgcacaga agatgtacgg cgtttgtcat
     2101 ctgtatatca cctgtaacgg gccccgccag cagcggttca agctgacggc tctgccggag
     2161 gcaaaggcac ggaaaatcgt ggaaacgctg gctcggacag aaatgcagga tatgtcttga
     2221 atcaatttgt aaaaccggtt tctgggggtg gaagccggtt ttttgttgtg aaaaatacta
     2281 aaaaatagtt gactttttac aaaatatgtg ctacaataaa agtgcaaacg atttgcataa
     2341 aaaaggaatg gaggaatgag aagatgcggt tatggaaaaa ggttctggct gcggcaactg
     2401 ccggtgtgct ttgcttggga agtgctggcg tgactggatt gcagaacacg ttggaatctg
     2461 tggggacggt attgtcggcg agtgcattgg tgccaaatga gtatgatgga atttgtggga
     2521 atttgtacta taagaattat tataatgatt tgatttccat agtcggttgt aatacagatg
     2581 ttgaatctgt ggaaattccg gctgagattg acgggaaacg tgtgacgagc attggagatg
     2641 atgcttttcg cgactgcacc agtttgacag aactcacgat accggacagc gtgacgagta
     2701 tcgggaggta tgttttttac ggctgtacca gtttgacagc agtcacgata ccggatggcg
     2761 tgacaagtat tggatggaat gttttttccg actgtaccag tttgacagca atcacgatac
     2821 cggacagtgt gataagtatt ggatggagtg ctttttacaa ctgtactagt ttgacatcaa
     2881 tcacgatacc agacagcgtg acggaaattg aagaaggtac ttttttcgga tgcaccaatt
     2941 tgaaaaatgt ttatatttct gatttggaat cttggtgtaa gattaatttc ggcggtttta
     3001 cttccaatcc attgtgctgt ggtgccaatc tttatgtaaa cagagagttg gcaacagaaa
     3061 ttacgatacc ggacagcgtg ataagtattg gagattatgc ttttgacggc tgtaccagtt
     3121 tgacagcaat catgatacca gacatcgtga cggaaattgg agagcatgct ttttacgact
     3181 gtaccaattt gacagaaatt acaataccgg acagcgtgac aagtattgga gatgatgctt
     3241 ttgataactg tactagtttg acatcaatta cgataccgga tagtgtgaca agcattgaac
     3301 aagaaacctt ttacaactgt accagtttga cagaaattat gataccggac agcgtgacta
     3361 aaattggaga tggtgctttt tgctactgca ccaatttgac agaaattacg ataccggata
     3421 gtgtgacaag tattggagag aatgctttta gaatctgtac caatttggca gcaatcacga
     3481 tattgaatgg cgtgacgagt attagaaagt atgctttttc tggttgtact agtttaacag
     3541 aaatcacaat accggacagc gtgactgaaa ttggagacgg tgctttttac gactgtacca
     3601 atctgacaat ctacggatat acaggttctt atgcagaaac ctatgccaag aaaaatgaca
     3661 ttccttttgt ttctctgaac aatatcacaa caactgaaac cactaccacc actatagaaa
     3721 caacagttac aacgaaagcg acaacgacta cggaaacagt cactacagaa gcgacatcta
     3781 caacgaaagt aacaacaact acagaaacag acactacaga agcaacatct acaactcaag
     3841 cgacaacaac tacggaaaca ggcactacaa aaacaacagc tgcaacgaaa gcaacgacgg
     3901 ctatggaaac agacactaca gaaacaacgg ttacaactaa aacgacaacg acaacaacaa
     3961 ttaccacaga agctaccacc accaccaaac aagattccga aacacctacc gtcatttatg
     4021 gcgacattac ccttgacgga cgtgtagata ttactgatgc tgttttgctg aacaagttct
     4081 gttctggtgc ggtgatcctg aacgatgctg ccaagctgaa cagtgactgt gacggcgacg
     4141 gcgatataac cggaaacgat gctgtagctc tgctgcaatt cttggtgcag atcgtacaga
     4201 agctgcctta tacagcataa ttctattgca ataacccaaa aaccggctcc cgcatttccc
     4261 atgcaggagc cggttttcga ttttcttaca tttgcagaat cgccgtcagg attcgcagga
     4321 tctttgcccc gtagccggca ccgcctgccc agcccctgcc gttgggattc tccggaatcc
     4381 ccagccactc cacatagggg gcacagcccc gctgcacata ccggaagcgg tcgtctacgc
     4441 aggcctgctg caaggctgcc gtgctggcat aggctttcag gtgctggatc tgtgcacgga
     4501 ttcccagctg cggtgtggca aaggaattgc cttttattcc gttgctggtc acgcccatgc
     4561 cgcagaaatt gttctgctcc agcgtcaccg ccgagccacg aaaggtgaag ttccccgttt
     4621 ccagacagga ctgtgcaaag gcaatgtccc cgcggatccc ttcggcagcc ccctcgctca
     4681 ggtagcaggg gatcatgtcc agtacgctct gtgccacatt gggattcact gtacggagat
     4741 aggcggtcat ctgtgcggca gtcgcctgtg tcgtgccggt gatcggcgtg agttcggtgg
     4801 tcagcctgcc gctggcatcg gcacggtaga ccacgccgtc cagcgtcacg gtgcggctgc
     4861 gaaccagctt gccgtcctcg ccgaaataat acaacgcccc gtcgatctct tgcagcccca
     4921 gcagggcggt ttcggtgtcc tcgctgaagt agtagctgct gctgccgtcc gtctgccagc
     4981 cggtctgtat ctcgccctct acattataat aggaaaggct gccgtcagaa aaggcgtgta
     5041 tccctgtgtc ctgcatgccg tatgtttccg agaagctgta gcggtgtccg tcgatttcct
     5101 gttcgccagt ttctttcagt ccgctgtcgt cggtgtaata gcggtagccg ccggactcca
     5161 cccagcccag aaccagttcg ccggtttccg gatcgtagta ccggcgttct ccgtcaactg
     5221 tccgccagcc ggttttctgg gtgccggtgt agtcgaacag ataggcggtg ccgtctatgg
     5281 tgacttcgcc ggtgaccggt gtcccgtcgg catcgtaata gcagcggctg ccgtccccgc
     5341 gggtttccca tgtgccttgg gtggttgcgg ttgccaccgg tacggcaggg aagtcctccg
     5401 ccgctgcgga aacaccgccc agcagacagg cagtccccag ccctgcggca agtgccagcc
     5461 agaatggaaa atgttgcaaa ataaacctcc ttgaaaaaat tggattttgc gttcgttatc
     5521 tattttacca tgctttttgc ggaattgcaa gtgcttttta gcatatttgc atgataaagt
     5581 gctgctgcat gcaacggaaa aatcgagatt tcgtcaaaat tacgggggaa cggcgaaaac
     5641 cggcttgttc tttggcggaa ttgtcgaaaa aaataacgca gactttgttg acaatacggc
     5701 aaaaatccgc tataatgtag gagaacagaa gtaataggat aagattgagg aaaacagggg
     5761 gataccggtg ggagaacgca ccggagaaaa ttgctgcgat ctttgcggca ggaggaattt
     5821 atgtctttac aggaaaaaga aacactggaa ctggcacagg caaaaatgca ggaatatttg
     5881 caggacaatg cggtctgctc catggacgag tatgttcagc acggaacgac cagcacatta
     5941 cagcactgcc tttcggtagt gcggatcagc tgtgccattg ccgttggtct gcatatccat
     6001 gtaaattatg aaaatctgat cctcggggca ctgctccatg atttctactt gtacgactgg
     6061 cacaaccatg tggacgaggg tgtgctccac ggctttgcac acccgcacat tgcctgcaaa
     6121 aatgcggcaa tgcggtttca tgtcaatgca gaggtgcagc atatcatcac cacacacatg
     6181 tggcccttga cgctgcggtt cgtgccccgt tccagagaag cggtgatcgt ctgccttgcg
     6241 gacaagtact gctccacgct ggaaacggca gcgggcatgg ttcatgcagc ggcagctctg
     6301 ttccggaaaa ggagggtctg acagatggta tacaatttaa cagaatgtgc tgcccttttc
     6361 ttcgcctatg ccgccatggg ctggtttatg gaagtcttgt acatgggcgt gcgggaacgg
     6421 catctcacca accgcggttt tctgctggga ccctgctgtc cgatttatgg agccggcatg
     6481 ctgggcatca ccatgctgtt tcagggcagc cactacggct attggcacgt gttcttctcg
     6541 atcattctgc tgtgcagtct gctggaatac gcagtcagtg ccctcatgga acgagtgttc
     6601 cacacacgct ggtgggatta tcacgatatg aagttcaaca ttcacggacg gatctgtctg
     6661 gaaaccatgc tgccgttcgg actgctgggt acatttgccc tctatcgcat caatccgttt
     6721 ctgatgatgc tgtttcaccg gatccccggc tatgtttccg gaaaactggt gactggtgta
     6781 gtcgttttgt ttctggcaga tctggtcatt tctgtttgcg tgctgcggaa tctgtccctg
     6841 ccggacggtg atgcagacca taccgcagag atctcccgcc gcgtctggga agatctgaaa
     6901 aagcggctgg gttaaggcga taccgcacaa agagcgtcta gcgactttgc acgccatctg
     6961 cttgcaaatt ttccgtcata tttcacaaaa atgctcgtga atacagcaag tattcactcc
     7021 gcttttt
//
LOCUS       DAWBGR010000132         7002 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2799_length_7002_cov_4.694544, whole genome shotgun sequence.
ACCESSION   DAWBGR010000132 DAWBGR010000000
VERSION     DAWBGR010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7002)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2799_length_7002_cov_4.694544"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1642 a         1719 c         2068 g         1573 t
ORIGIN      
        1 ccctttaggg gagggggacc agcgaagctg gtggaggggt tctttgtgga atcttctgct
       61 tggtgtaaaa gatttatgta gcaatccctc agtcagcctg cggctgccag ctcagcacaa
      121 ggcacgccct gcgggtgcct tttcagggga gcctgataga aaaaaagcct cccttgaaaa
      181 gggaggggga ccgtcgttag acggtggagg gattctcacg tggaatcttc tgcacaatca
      241 aatcatcatt tatcattcca ctctctcaga gaggagcaaa aacattcatg aaaaagtatc
      301 gtgagtccat tatgaaagtg gtctttctgg tggcagcctg cgtgtccatt ctggcggtgg
      361 tgctgatctg cggattcctg ttcgtcaacg ggttccccgc cattgcagag atcggaccgc
      421 tgaaattcct gaccggcgtg atctggaagc cgcaggaggg cgagttcggc attctcccca
      481 tgattatcgg cagcatttac gccaccggta tcgctattct ggtgggggtg cccatcgggc
      541 tgctgtgttc cgtctttatg gcgagattct gcccgcaaaa gctgtataag atcctgaagc
      601 cggcgatttc tctgctggcg ggaatccctt ccattgtgta cggattcttc ggactggtgg
      661 tcattgtgcc gatcatgcag agcattttcg gcgtgggcaa aagtgtgctc actgcgggca
      721 ttctgctggg cattatgatc ctgcccacca ttatcgaaat ttccgaatcg gcgatccgtg
      781 ccgtgcccaa cagctactac gaaggctcgc tggcactggg ggcgacacat gaacgcagcg
      841 tgtattgtgc caccctgccg gcggcaaagt ccggcatcat ggcaggcatc attctgggca
      901 tcggcagagc cattggcgaa accatggcgg tcgtcatgat cgccggcaac cagaccgtga
      961 tccccaagag cctgctgtcc ggtgtccgta ccatgacagc gaatatcgtc atggaaatgg
     1021 gttacgcaac agacctgcac cgaggagcac tgatcggtac cggcgtggta ttgttcgtct
     1081 ttatcctgct gatcaatgtt tgcttctcgc tggtgaagaa aaaggagcag aaatgaacgt
     1141 ggaaaataca gcgaagatcg tgggcatcaa ccaccagacc ttccggcaga aaatggcttc
     1201 ttataagcgg caccccggct cactggtggt tctgcttctg acgattctgg cggcagtcat
     1261 tacggtaggg gtgctgttga gcctgatcgt ttatatcgtg gtcatgggcg tgccgaatat
     1321 ccgcctgtcc atgttcgcat ggaagtatac gaccgaaaac tgttctatgc tgccgtccat
     1381 tctgaatacg ctgatgatga cagtgctgac actggtgatt gccgtgccta tcggagtgtt
     1441 ctccgccatt tatctggtgg aatactccaa ccgcggcagc aagtttcaga tgacactggt
     1501 gaagatcatt cgtatgacca ccgaaaccct acagggaatc ccgtccattg tgttcggtct
     1561 gttcggattt ctggcgtttg ccgtgacctg taagttcagc tattcgttgc tgggcggtgc
     1621 aattacaatg gcactgatgg tactgccgct gattatgcgt accacggaag aagccctgtt
     1681 gagcgtgccg gattcctatc gtgagggcag cttcggactg ggtgccggac ggctccgcac
     1741 catcttccgc attatcctgc ccagtgctgt tcccggcatt ctggcgggca tcattctggc
     1801 ggtcggtcga atcgtcggtg aaaccgcggc tctgatgtac accgccggaa cgtccaccga
     1861 tgtctgcacc ggtttcctga aaccctgccg gacgctggcg gtacatatgt accagctgct
     1921 gtgcgagggc aaatacatgg gtgcggcata cgccacggct gtggtgctgc tggtcatggt
     1981 cattctcatc aatgccctgt ccggctatgt ggcaaagaag ctgacgaaaa agtgagtgtg
     2041 ggttcggcgg aatcttttgc ttggtgtaaa gggtttatgt aataatccct cagtcaagcc
     2101 tgcggcttgc cagctccctt ttcaagggag cctagtggaa aaagcctccc ttgaaaaggg
     2161 agggggacca gcgaagctgg tggaggggtt cttgtggaat cttttgctta atgtgtgaga
     2221 ttttacggaa gcacccctca gtcacctgcg gtgacagctc agcacaaggc acgccctgcg
     2281 ggtgccctaa aggggagcct aaacaccaaa agggagccta gatataaaaa accgcccacc
     2341 cacatcttat gaggagaaaa acaactatgg caaaatttgc gattcacaac atggacttgt
     2401 actacaacac gttccatgcc ctgaaaaata tcaatctgga attgccggaa catgaggtca
     2461 gtgcctttat cggcccctcc ggctgcggca agtctacgct gctgaaatcc ctgaaccgga
     2521 tgaacgatct ggtggagggc tgcaagatca ctggtgacat tcgtctggac ggcgaggaca
     2581 tctacggcga tatggacgtg aacctgctgc ggaagcgggt gggtatggtg ttccagaaag
     2641 cgaacccgtt ccccatgagc atctacgaca acatcgccta tggcccccgt acccacggca
     2701 ttcattccaa ggcgaagctg gacgagattg tggaaaattc cctgcggagt gcggcgatct
     2761 gggacgaggt caaagaccgt ctgaacaaga gtgcactggg catgtccggc ggacagcagc
     2821 agcggctctg tatcgcacgg gcactggcag tacagccgga agtgctcctc atggacgaac
     2881 ccaccagtgc actggatccg atctccactt ccaaggtgga agatctggtg ctgtcactga
     2941 aaaaggacta taccattatt attgtaaccc acaacatgca gcaggcaacc cgtgtgtctg
     3001 accgcaccgc gttctttctg ctgggagaag tggtggaata tgccgaaaca gaacagctgt
     3061 tttccaagcc gcaggatcag cggacagagg actatatttc agggaggttc ggataatgag
     3121 agaacactac gaagcacagc tgacaacgct gaataccgaa atgattcaaa tgggagcact
     3181 ctgcgaagat gccatttccg gtgccattca ggcactgctg gaggacgaca agcaccttgc
     3241 cgaaaaagtc ggcagcattg aaatggagat cgaccagcac gaacgggata ttgaacggct
     3301 ctgtatgcgg cttttgctga tgcagcagcc ggtggcgacc gacctgcgtg tggtttcttc
     3361 tgccctgaaa atgatttctg atctggaacg tatcggcgat caggcgtttg atatttcgga
     3421 cattaccaag cacgtttctt ttcaaaagta tgagagcaag gtacacatca aggaaatggc
     3481 aaatgctgtc attcacatgg taaccgacag cgtggattcc tatgtgaaaa aagatgcggc
     3541 actggcgaaa aaggtggctg ccgcagacga tcaggtggac gatctgttcc tgaaagtccg
     3601 ttcggaactg gtggaactgg tgcgttcgaa ccaggacatg gcagagggtg cactggattt
     3661 gatgatgatc gccaagtatc tggaacgcat cggcgaccat gctgtcaacg tggcggaatg
     3721 ggtgatttat gccattaccg gcgaccatat gaagctggcg tgaggggagt tgcccttgaa
     3781 aggtggaggg gttctcgtgg aatcttttgc ttggtgtgtg aggtgtcacg gaagtactcc
     3841 tcagtcaagc ctgcggcttg ccagctcccc taaaggggag cctgaatgta aaaagcctcc
     3901 cctgaaaggg gagggggacc agcgaagctg gtggaggggt tctgtgaaat cttttgctta
     3961 atgtgtaagg tattacggaa gaaatccctc cggcacttcg tgccacctcc cttttcaagg
     4021 gaggctgata aaaacggaat cctattttaa ctggggaaac cccgaaaatt tccttgacga
     4081 atgacgggga atatggtacg atatagacgg taaaacgtca ggagggagca tctttttgga
     4141 cgttccctct ttttgttgaa atgacagatg cagagccggc ggaacggctg cacaggcagc
     4201 actgctggta cactctgcgg gaagaaaggg cagtcatatg atttatttag tagaagacga
     4261 cgacagcatt cgggaattgg tgatctatac cctgcggagc accggcatgg acgcaaaggg
     4321 gttcgggctg ccctcggagt tctggcatgc catggaagaa caactgccgg aactggtgct
     4381 gctggatatt atgctgccgg aagaagacgg tctggagatc ctccggaaaa tccgcggcag
     4441 gcgggaaatc aaaaatctgc cggtcattat gctgactgcc aaggggacgg agtacgatca
     4501 ggtagtggga ctggacaacg gggcagacga ctatgtggca aagcctttcg gcatgatggc
     4561 actggtggca cggattaagg cagtgctccg ccggacagcg gaaaagtccg cagagggcaa
     4621 ggtgctccag ctgggcgaac tgcgggtgga tccggttcgc cacgtggtac aggcggcagg
     4681 ggagccggtg aacctgaccc tgaaagaatt cgaactgctt tgcctgctgc tggacaatcc
     4741 gggtgtcgtc tttacccgcg accagctgct gaaccagatc tggggctatt cgttcgacgg
     4801 cgaaagccgt accgttgatg tgcacgtgcg gcacctgcgg cagaaactgg gggactgcgg
     4861 gaagtatatt gaaactgtcc gcggcatcgg ctaccgttcg ggagatggaa acgcatgacc
     4921 aggaaaattt ttcaatccat tatcgccgtc gtgatctctg tgctgctgct aagtctggca
     4981 ctgattaccg gcgtgctgta taaccacttc gaaaccacta tgctggatca gctgcggacg
     5041 acggcacagt ttgcggaaca gggcgtggaa caggagggca tggcgtattt cgacagcctt
     5101 cacgcccaga actgccgcgt gacatggatc gccgcagacg gcacggtgaa atacgacaac
     5161 cgcagcaacc cgaaaaccat ggaaaaccat gccgaccggc aggaggtgcg ggaggcaatg
     5221 gagaacgatt ccggcaccag tgtccggcgt tcatccaccc tgtcggaaca caccatgtac
     5281 tacgccaagc ggctgagtga cggcacggtt ctgcggctct ctatgtccca gcgttccgtg
     5341 ctgttcctca tgggcggcat gctttcgccg ctggtgttta tctttctggc agcctgtctg
     5401 ctggcgggcg tgctgtccta tcgggtgtcc aaaaaaatcg tgaagcccct gagcgagatc
     5461 gatctgaagc atccggaaca ggtggaaacc tatgacgaac tctccccgtt tttgcagcga
     5521 attgccgccc agaatcggga gatcgatgcc cgcatggcgg agatccgcaa gcaacagcag
     5581 gagttctcca tgattaccga gaacatgagc gagggcttgt tcgtggtaga ccggaactat
     5641 cagattttat cctataataa aagtgccatg cagatcttcg gcatggatcc cagacaggaa
     5701 catgaaaatc tgctggcagt gaaccggagc gagggcttcc gcaacgcggt ggacagtgcc
     5761 ctgaaaggtc gccacacaca ggaaaatctg gagctgaacg gcagagtgta ccagatcatc
     5821 gccaacgccg tctgccagcc tgattttgcg gaagatatgg tgggagctgt gattctggtg
     5881 ctggacgtga cggagaaaga ggcacaggaa cagtaccggc gggagttcac ggcgaatgtt
     5941 tcccacgaac tgaaaacgcc gctgacttcc atttccggca ttgcggagat cattcgtaac
     6001 ggcattgtca agccggagga tattccccat tttgcaggga aaatttacga cgaatcccag
     6061 cgtctgatca ccctgatcgg ggacattatc aagctgtccc gtctggacga aaatcaggtg
     6121 cctatggaac gggaaacagt ggacatgctg gaaatggcac gggacgtggt gcagcagctt
     6181 tcctccgttg cccgcaaaaa cggtgtcacg ctggtggcaa acggcaccca cgggcaggtg
     6241 cagggcgtgc ggcaggtgct gggggaaatg gtgtataacc tctgcgaaaa tgccgtgaag
     6301 tacaaccgtg ccggcgggcg tgtctgggtg gacgtgcagc aggtggcaga ccatgtggtg
     6361 ctgcgggtca aggataccgg tatcgggatt ccggcggcgg agcagggacg catttttgaa
     6421 cggttttatc gggtggacaa gagccactcc aaagcggtgg gcggcaccgg actgggactg
     6481 tctatcgtca agcacggggc agctcttcac catgccacga tttccgtcag cagcgaaccg
     6541 gagcagggca cggagatcac actgacattt ccggcggcgt gatgcggcgg ggaatgcatg
     6601 ggaaatccac atgattttgg aaaagaaaaa gagagcagac cgtgaggcgt gctctctttt
     6661 ttgatatgtg ttgttggggt gatgctttct tggcgatttg cagacgaatg agcgtttgac
     6721 atttttcgcg tagtgtgata taataaaaaa ccgagggcat acctgaaacg gtaggcggtc
     6781 aaatccacgc tcccacaatc ttctatttga attgatggga ggtgatgcat gatgatggat
     6841 gtaatttcct ttatcgtaag cattttttgt aatgtctttc gtgcagttct tgcatactta
     6901 gcatacaaaa agaaataacc gccctgaagc taccaacttt ggcgattatt tcttagtcgt
     6961 ttgaatgggg agcagaccgt ctactggtgt gctctcttta ct
//
LOCUS       DAWBGR010000133         6976 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2804_length_6976_cov_3.665222, whole genome shotgun sequence.
ACCESSION   DAWBGR010000133 DAWBGR010000000
VERSION     DAWBGR010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2804_length_6976_cov_3.665222"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1664 a         1816 c         1638 g         1858 t
ORIGIN      
        1 ccttagcgaa tatcgacaaa agcaccggac gaaatcgctc cgtccggtgc ttttgcggaa
       61 tgttcttttt gaatatgttc ctgttggcag ctgctacagt tcacgatcaa acaagaattg
      121 aaacagcaca ggcgtaatgg caggataggt cagattgtca ggcagcgagg agaacgcctt
      181 tcgttctgcc atttcacttt cgggaatgtc ggaaaattgc cgaatcaccg catggaatac
      241 aacgccgttg gcaccatgca gagtattttc gtcccaggca cgataatcgc aaattggttc
      301 gatgtcaaaa tctacggcac cagactcctc aaacagctct cgttttgctg cttccagcgg
      361 tgtttctccg gcttcaatat gaccgccctg cgtttcccat gtggttcgtt ccttgtgccg
      421 gctcaaaatc atttttccat ggctctcgga aaggatgacc acatacttgt aatttttcag
      481 agcatttatc tcgtaggtct tacaacgcat aatcagcttt gattttcctt ttcgatcaga
      541 taatcgccgc agtacatctt ccgcagcttc ggcttctcga ttttgccggt cgcgttgcgg
      601 ggaaccggtg cgaaaatgat tttccgcggt cgtttatagc ggggcatgcc catgcagaat
      661 tcgttaactt ccgcctcggt gcattccacg ccctgcttta gttccacaat tgccgcagca
      721 atttcgccca gccgcttgtc cggcagtccg atcacagcca cgtccttgat cttgtcaaac
      781 ttgctcataa agttttcgat ctctaccgga taaatatttt cgccgccgct gataaccacg
      841 tcttttttcc ggtctacaag gaagtagaat ccgtcctcgt cctgccgtgc catgtcaccg
      901 gtccagagcc agccgtcgtg cagaacttcc gcggtcgctt cgggattgtt gtagtagcat
      961 ttcataacgc cgggaccctt gactgccaat tcgccgactt caccctgtgc caccggttct
     1021 ccgttttcgt caatgatccg gacttcccag ccatagcccg gaatgccaat tgctcctacc
     1081 ttatgaatat tttccagtcc cagatgcacg cagcccggac caatggattc gctcagacca
     1141 tagttggtgt cgtacatatg attcgggaaa tattctttcc agcgtttgat cagactctgg
     1201 gggacaggct gtgcaccgat atgcatcagc cgccactggg agagttcata gttttccaga
     1261 cggacagagc cgttgtccaa tgcttccaga atgtccagtg cccacggcac cagcagccag
     1321 acaatggtac acttttcacg ggaaatagtt tccagaatgg tttccggatt gactcctttc
     1381 agaagcactg cacgtccgcc ggtgagcagg ctgccgaacc agtgcatctt ggcaccggtg
     1441 tgatacagcg gcggaataca cagaaagaca tcgtccttgg tctggtgatg atgattctgc
     1501 tctaccttgc aggcgtgcat caggctttcg tggttgtgaa gaattgcttt cgggaaacca
     1561 gtcgtaccgg aggagaaata aatggcagcg tcgtcctcat ctgttagcgg tactccgggg
     1621 gagatgctgg cacagttgga ggtcagcttg tcatagtcct ctgcaaacga ggggcagtcg
     1681 ccgccgacat aaaagaggat tcgcttcttg ctgatcgatt ctgcaatggt ttcaattcgt
     1741 ccgataaatt ccggaccaaa caccagtaca tcggcttctg caaggtccag acaatattcg
     1801 atttcttccg cactgtaccg gaaattcatg ggcactgcca gagcaccggt tttcagcgtg
     1861 ccgaaataaa tgggcagcca ctccaaacag ttcatcagca gaattgccac cttgtcgccc
     1921 tttttcacgc cgcggctcat gagcagctgt gcaaaacggt ttgccttttc gtcaaagaca
     1981 tgccatgtga tttcccgacg ataccagaaa gacgaggtag gctggatcag gtcgtagtcc
     2041 ttccagagca cccgtcgtgt ttcctgtacc tgcggattta cttctaccag tgccacatca
     2101 ttgccgtact ttctggcgtt ttcttccaat aattctgtga tcggcatatt cgttactcct
     2161 gtccttgttc tgtatggttt ccgttatatg catagccagt gagaaatgcc ttctggttga
     2221 tgtcaatggt tttcggcggc acagtatctg caatgacctg taaccattct tctttggaga
     2281 agtgcatgtg ctgtgcagct acgcccagaa tgatcgtgtt gaatacccgt gcattgccca
     2341 gcttcagagc ctcggcggta gcatctactg ccagcacatt gtattttttg ctcagctcct
     2401 ccagaatgtg ttccggatac tctgcggcac cgatcaccac cggcatcgga tcaatgcggg
     2461 tgtcgtttac gataatggca ccgccctttt tcaggaagtg tgcataccgc agtgcttcca
     2521 gccgttcaaa agcgatcaga atgtctgcct gtccttcttc tacgatcggt tctgccacag
     2581 attcgccgta gcggacaaat gttactacgc tgccgccacg ctgtgccatg ccatgcactt
     2641 ccgaaagctt tacatcatag ccgccagtca gtgcaatctt tcccagcacg cggctggtca
     2701 gcagggtgcc ctgtccgccg acacctacga tcataatatt gatagtctgc atcatgcctt
     2761 gcctcctttt tcccgcggga tcagttcgat tgcgtggaac ttgcaccgtt ttgcacagag
     2821 ttcacagccg ttgcacatcg ccgcatcgat tttcggaatg ccgtttgcct gtttgaccat
     2881 tgcagggcag cccgggacca gacactgtcc gcacttcttg caggcatctt catggagaat
     2941 gcacttgccc ttcggaacaa attctttcag cagggcacag ggggacttgg tgatgataac
     3001 agacaaagcg tcccgctggg tttcctgttt cagcagcttt tccagcccct caatgtcgaa
     3061 cgcatcgatt tcatagacgt gttcaatgcc cattgccttg cagaggtgat agatgttgat
     3121 cgcataggtc agttcaccct gcaaggtctt gccggtagcg gcgtgatcct gatgtcccgt
     3181 cataccagta gtggagttgt ccagaatgat gaccgttccg gttgctttgt tgtacaccat
     3241 gttcatcaaa gagttgacac cggtgtgcag gaacgtagag tcgccgatca gtgccaccca
     3301 gtttttcaca tagtccttgc ccttggcttt ttcaattccg tggagagtgg aaatgcttgc
     3361 acccatgcac acgttaaagt ccataacacc cagcggcgga acggctccca gtgtatagca
     3421 gccgatgtcg ccggcaccgt gcagtttcag ctttttcatt acagaaaaga cgctgcggtg
     3481 cggacatccg gggcagagga tcggcggacg gttcggagcc tgtgccggcg gtgtcagctg
     3541 gacatcctca tgcagaatgg ctttccgcag catattggca ctgtactcac cctgtactgt
     3601 aaagatctcc ttgccgatta ccggaatacc ccaagaacga acttgttctt cgatgaccgg
     3661 ttccagttct tctacaatgt acagtgtttc cacattggca gcaaattctt cgatcagctt
     3721 tttcggcagc gggtgtacca gaccaagctt cagcacagat gtgtccggca gggcttcccg
     3781 tacatactga taggggatac cgctggtgat gataccaatt ttcttgtcgc ggtattccac
     3841 acggttgatg tcaaagtgac aagcatcttc cgccatctgc ttcagccgct gttccacgat
     3901 cacgtgccgt ttcttcgcat tcgccggcat cagtacattt ttctcgatgt tcttttcata
     3961 gggcttgtcc ggtacgtttt cccgttcctc cggatgtact acaccctgtg aatgggacag
     4021 acgagtggta gtgcggacaa tgaccggcgt gtcatatttt tcactgtatg taaacgcaag
     4081 cttggtgaaa atgcgggctt cttcgctgtc ggaaggctcc agcaccggaa ccattgccgc
     4141 acgtgcaacc atgcgggtgt cctgctcgtt ctgggaactg taaatgccgg ggtcgtccgc
     4201 agcaaccagt accagaccgc cgtttacgcc ggcataggaa atcgaataca gcgggtcgga
     4261 agctacattg acaccgacca gtttcataga agccattgcc cgtgcaccgg cgatagaggc
     4321 accgattgcc acttccatag ccaccttttc attgggtgac cattctgcat agacttccgg
     4381 atattttgcg atacattcgc ttatttcagt gctcggcgta cccggatagg cagcagaaac
     4441 cttgacacca gcttcatagg ctccctgtgc aatggcggca ttgccaagca tgatttttga
     4501 attcttcaac agattttccc ttctttcgaa tgatttctgc catacaggct gtaaagcaga
     4561 caagaaaccc gccttttgag aaaaagacag gtcgttcaaa gcagcactat ctttacaata
     4621 agtaagtagt ataataaata agtagtgcag ccttttctat tgtaatacat tttcgaaaaa
     4681 atcacaaggc ttttttcgac ttttggtgat tttgcatatc tccggcaaag tttcttgtac
     4741 attttcacaa aaagtaaaaa gcccgaaaac accaaacggc attttcaggc tttccggaaa
     4801 cacgctctgt ttcgggatta ttcaaaggaa tccagatcga tgccagccag caggtctttc
     4861 agtgcagcca gttcgtcggc ggtcagtgtc acgccttttc ccattctgct gtgatccgga
     4921 gcccagtcac gaatatcgta cttcggttcc ttttcgttcc agctgatccg gttgacttcc
     4981 ttgctccagc ctcttgcatt ttcacccaga gtacccagat tctctacgat ttcatatttc
     5041 agttctgcca taataaaaac actccttgaa aaaatttgat gcaggtgttg cctgaagcgt
     5101 gcacgtgcct acacgcacac gctgcatctc gaatacctat tatacaagat gcgttccgaa
     5161 aatgcaacac cccgccgccg gcttttttgg aaattggaac tttcactaaa aacattaagc
     5221 ttatttcaag caacatacgt tataatgaca acaacatcaa gcgaaaacag ccatttcgca
     5281 cagaagggag gaaacctgta ccggaaaacc tgattccggc agcaggatat atatgggtgc
     5341 aattcagaca ttcgcacgaa aggaaatcaa gtttttactg acagaacagc agtatcagga
     5401 ttttctgccg cttctggaac actacatgaa ccccgacaaa ttttgcgtcg ggggaaaaga
     5461 gtatggcatc tataacgtct attatgatac accggacgat tttctgattc gggaatccct
     5521 tgaaaaaccg gattacaagg aaaagatccg gttacgcagc tatctctcgc cggcacagcc
     5581 ggaggacacc gtgtttctgg aaatcaaaaa gaagatctgc ggcattgtca acaaacgccg
     5641 cgttaccatg acactggcac aggcggaggc ctatttgcag gaaaagattc accctgactt
     5701 aggcggttca tacttgcagg agcaggtcat ggacgagatc gactttttcc gccagcggta
     5761 tgagatattc ccgaagcagt acatcagtta tcagcgtgcg gcgtatttcg gcaaggaaga
     5821 taccgatttc cggctgactt ttgatcgggc actgaccgga cgacgcagcg acctctcgct
     5881 tcgccagcag agttatggtg acctgctgat tccggaacag atgcgtctga tggaagtcaa
     5941 gatcgcagac tccatgccca tgtggctggc agaagaattg gcaaaactgc aagtgtataa
     6001 aaccagcttt tccaaatacg gaaaggcata tcagatgtac gtacaaaatc agctggaatg
     6061 gactcgtccc cagcttacca tagaaaaaag gagacgttac gattatgctt aacaccatta
     6121 ccaatgtctt gacggcgacc agttccgaat tatcttccct gctgtcgaat gtaacagcga
     6181 ccacaagcac cgcttctgta ggagatatgt tgtttgccct gattctgggc gttgtcatgg
     6241 gctttctgat cagtctggtc tacatcttca cccacaagaa atccggctat acccagagct
     6301 atgttatgac attgctgatg ctgccgccga tcgtggcaat tgtgctggtg atgatcaact
     6361 caatggcaag tgccctgagc cttgcgggcg tctttaccct ctgtcggtat cgtactgttc
     6421 ccggtgaccc gaaagatatt acctatgtct tttttgcaat ggcaaccggt gtgatctgcg
     6481 gcatcaacgg cggcggacat gtgtggtttg tctttgtgtt cttcgcagtg atcgcggcag
     6541 tgctgattct gatagaggtg ctgcggtacg gcatctgcaa aaccagcagt atgacactga
     6601 aagtgaccat tccggaaaaa ctcaactata tcggactgtt tgatgacatt cttgcagaaa
     6661 acacgaccag ctggcacctg aagcgtgtca agaccaccaa ctttggctcg ctgtttgaac
     6721 tgatctacag cattcagctg aaaaacgacg tagaccagaa aaaattcatg gacgagattc
     6781 ggaagcggaa cggcaatctg acagtaattc tttccctgta ctgttacgaa gatcaggttt
     6841 atgaaaaata agaccttttt ttcaaaatag aaaaacgaca tattggaata aatttttcat
     6901 acattccttt aaaaccgtgg atttatccgc ggttttcttt tcttttcaaa aacttgcata
     6961 ctaaactata taattc
//
LOCUS       DAWBGR010000134         6964 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2813_length_6964_cov_5.886959, whole genome shotgun sequence.
ACCESSION   DAWBGR010000134 DAWBGR010000000
VERSION     DAWBGR010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2813_length_6964_cov_5.886959"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1775 a         1451 c         1802 g         1936 t
ORIGIN      
        1 atgacaaaaa agcggagaac gtccaaaacg ttctccgctt ctcatttctc tttgcgtgaa
       61 gcctaccgct tacagagccg cgataacttc cacttctacc agcacaccct tgggcaggtc
      121 tgctacgcca acacagcttc ttgccggctt tgcatcgccc atggcttcgg cgtacacttc
      181 attgaatgca ccgaaatccc gtgctacgtc cttgaggaag caagtcgtct tgacaacctt
      241 gtcgaagctg gtgcctgcgg cttccagaac ggctttcagg tttgccatga cctgcttgga
      301 ctgtcccacg atgtcagttg cttctacatt gccggtcgcc ggattgatgg caatctgacc
      361 ggaagtatac agcatatcac cgattttgac agcctgtgaa tacggaccga ttgcgtttgg
      421 tgcgttcttg gtataaatgg tatccatgat aaaaacctct tttcaaaaaa tagggatttc
      481 ttaggcaata ccgcacaaag attgtgcgac agatgcgttg tcaaattttt cgtcagatga
      541 aacaaaaagc ggagtgaata ctggatgtat tcacgagcat ttttgtgaaa tatgacggga
      601 aatttgcaag cagatggcgt gcaaagtcgc cagacgctct ttgtgcggta ttgccttatt
      661 ttagtccacc agatggaatc ctgcttccat gagggcactg cggatctcct gaatgtgtgc
      721 gtaatctctg gtttccatgg tgattcgcag gaagcagccg ttgatgtcgc tgccctcgtt
      781 ggcacgctcg tggtgaatgg agatgacgtt gccgcccaga tcagcgatga tgcgggaaac
      841 atctttcagc tgacccggct tgtccaacag ttcaatgttc agcatgcagc ttctgccgga
      901 catcagcaga ccacgcttga tgacacggga cagaatagtc acgtcgatgt tgccgccgga
      961 aaccagacaa accactttct tgccctctac gggaaccttg tggaacattg ccgcagccac
     1021 agatgctgca ccggcaccct ctgcgatcag cttctgcttt tcgatcagag ccagaattgc
     1081 agcggagatc tcgtcgtcgg tcacagtcac aatgtcgtcc acatacttct tgacatactc
     1141 gaaagtgtga ggccccggtt ccttgacggc gataccgtcg gcaatggtgg aaacggagtc
     1201 caggcaggtg atcttgtcgt tgtgaatgga ctcgtacatg ctgggtgcac cgcttgcctg
     1261 tacgccgtaa accttgatat tcgggttcag ctgcttaatg gcaaatgcca cgccggagat
     1321 cagtccgccg ccgccgatcg gcacaatgac tgcgtccacg tctgccatct gatccagaat
     1381 ttccagaccg atggtgccct gtccggcaat gacgttttcg ttgtcaaagg ggtgtacaaa
     1441 agtatagttg tgttccttct ggagctctac agccttgttg taggcatcgt catagacacc
     1501 cttgaccatg cagacctctg caccgtaact cttggtagct tctaccttgg agatcggagc
     1561 accgtccggc aggcagatga gagccttcat gtggttcttt gccgcagcca gagccacacc
     1621 ctgtgcgtgg ttgccggcag agcaggcgat gacaccccgt gctttttctt cttctgtcag
     1681 ctgggacatc atgtaatagg caccgcgaac cttgaaagaa ccggtgacct gcaggttctc
     1741 tgtcttgaga aacagattgc agtccggaca gagtttcggt gcctgaatca gatccgtttt
     1801 ccggatcacg ctccggagca cgtggcttgc ttgatagatt ttatccaatg tcagcatgga
     1861 taggacttcc tttccataat catattaaga acgctgtgtc agcagctggt cgatcagctg
     1921 ctttgcagca cgggcacttc tgccgctttt ctggagggcg aactgttctg cctgtgcgtc
     1981 cagttcctcc atggtgatgt ccagaccgtt ctgttctgcc agagcgtgga cgatctccag
     2041 atacaggttc ttctgcgggc ggctgtaggt aattgtgatg ccaaatcgtt ccgacaggga
     2101 gatgcgttcc tgtacggtgt cgttggagtg cttgtcgctg ccgtcgtcaa aggtttcctt
     2161 gaccagatgc cgccggttgc tggtggcgta gatgacggta ttccgcattc tggcggcaat
     2221 gctgccctct agagtcgctt tcagcgtgcc gaagttgggg tcgtcctcgg agaatgtgag
     2281 gtcgtcaata aagaggataa acttcagcgg atttttgcac aggtgatcca tgatctgggg
     2341 aatgtagcac agttggtctt tgttcagttc gatcagccgc agtccgtcat ctttgaacgc
     2401 gttggcaatt gccttgacag ttgcagattt tcccgtgccg gcatctccgg cgaggaggac
     2461 gttggaggcg tattttcctg caaggagtgc cttggtattc tctaccacaa tgccgcgttc
     2521 ccgttcgtat cccaccattt gttccagaga aatggggtca ggatactgca ccggaaccaa
     2581 ctcgccgctt gctccgacgg tgaacatgtg gtgccgtgca tagatgccgt agccgtgctt
     2641 gtcgatctcc gctacacgct gggtgtagag cttgtccaga tcggtcttgt gggtctgcca
     2701 tgacggcagg ggaatgctgg tctgcatctc tgcctgaatc atggcagggg tgagcattgc
     2761 aatgcggttc agcaatttca attctgtgtg caggcaggtt tccatgttct gcgaaatggt
     2821 ttcgccggca ccggcacgct ggacataaac gttttcgtgt tccagaacga tctgttccag
     2881 atagccggtg aggtcgtctg cccgttcata cagggcggaa accatggcac tgtaaaagcg
     2941 gatctgtgcg ggggtatttt ccttgtcggt gtggcagaac tgcatcagtg cacgaagcac
     3001 cggatcctgc tgtaaactgc ggaaaatgac aagggcatcc agcccgtatc cgatttcttc
     3061 tctgggagtc atatggagtc atctctttcg gtttttatgt tagtatcagg gaaacaccgc
     3121 ttacttcagc agtgcaatga tcttttcggt gcattcggtg caggacagcg gtgttacgcc
     3181 ctcgccagcc agatcggcag tgcggtagcc tgcattcaga tatgcgtcca cagcgtcttc
     3241 gattgcctgt gcctcgtcca tgaggtcgaa ggagtagcgg agcatcatgg ctgcggagag
     3301 aatggttgcg atggggtttg ccttgttctg tccggcaata tccggtgcag aaccgtggat
     3361 cggctcgtac atgccacgct tggtggcacc cagagaagcc gacggcagca taccgataga
     3421 accggtgatc tgggaagctt catcggacag aatgtcgccg aacatattgg aggtgacaac
     3481 tacgtcaaac tgtctgggat tcttgaccag ctgcatggct gcgttgtcta ccagcatatc
     3541 ggtgagggtg acatccggat agtcctttgc cacttcgtga acgacttcac gccacagacg
     3601 ggagctttcc agaacgtttg ccttgtcgat gctgatgacg ttcttgttcc gcttccgggc
     3661 ggtttcgaaa gccacgcgtg cgatacgctc tacttccatt ttgctgtagc actcggtgtc
     3721 atatgcttct tcgccgtact tgccctcgcg tctgccccgt tcgccgaagt agatgccgcc
     3781 ggtcagttca cgaaccatca tgatgtcgaa gccctttgcc acgatgtctt cccgcagggg
     3841 gcagtcgcct ttcagagccg gatacagctt tgccggacgg aggttggtga acagtcccag
     3901 agcctcacgg attcccagca gagccttttc cggacggaga tgcggcggca tctgatccca
     3961 cttgttgtga ccgactgcac cgccgacagc acccagcagg acactgtcgc ttgccagaca
     4021 gccgtcaatg gtttctttcg gcaggggaat gccggtggca tcgatcgcac agccgccgat
     4081 gaggtacggg gtgcaggtga aggtgtgacc gtatttcttg ccaatcttgt ggagaacagc
     4141 cacagcactg tctacgatct ccggaccgat accgtcgccc cgaagcaatg caatgttaaa
     4201 attcatggga aagactcctt tgtgtttggt ttggcggaac aaatcgttga cataccccat
     4261 ttgttccgca aatactcttt cattatacac caaagctgtg aagattacaa ggtttttgtg
     4321 aaaattctgc atttcgccac aaaaaagccg gtgcggaggg ggctgtgtcg gtgattttgg
     4381 ttgtggattg acttggttgg ggctgtgtgg tataatgaag gtgtggtaaa tcggaatttg
     4441 taaatctgat tgattttata atacaaggta ttggagagaa ataatatatg ttagatttaa
     4501 aaatcaaata caacgaacta acagcagagc aatttattga attatgggaa acagtatggg
     4561 gagatggacc atctcttgag caaacaagat tagcaatgaa acatacccta tttagagttt
     4621 ctatatggga cggagattgc attgttgcta tggcaagaat gaatggtgat atgggactta
     4681 attattatat taaagatgtt gttgtcagac ctgaatatca gggaaaaggt attggaagaa
     4741 tgttaataga tgaattaaga aggtttataa ataacaatgg agtaaaagga acagatattt
     4801 ttgtggagct atgtgcggtt ccggataaga taccttttta tgaaaaattt ggatttgaag
     4861 caaatgaggc acaaagatta aaaaaatttt atcatgtcga gtaatattaa attccagttt
     4921 ttctacatga gaagaacgca tagcgggcaa ctgtgtcggt gaaatttgcg gcggattgac
     4981 tgcgttgggg ctgtgtggta taatggaaat gcggtaaatc ggaagttgtg gaggaatcat
     5041 tgatgatact tttggtagtt gatacacaaa aagcaattac aaatgataaa ttataccaat
     5101 ttgatttatt caaatctcgt gttatagaac tgataagaac agctcgaaat aataagatag
     5161 aagttatttt tgttcggcat gacgatggga ttggcaatga attgacaaaa ggaaatgatg
     5221 gttttgaaat atatgatgga tttcaaccac ttaatggtga acttgttttt gacaagaacg
     5281 taaatagtgc ttttaaagat actggactat tggagtattt aagacaaaaa gaagaaaata
     5341 cgattattat tgtgggattg cagacagatt attgtattga tgcaacgatt aaatcgggat
     5401 ttgagcatgg tttcaaaatg attgttcctg caaatgcgaa tagtacagtt gataatcagt
     5461 atatgaccgc tgaacaaagc taccattatt ataatgaatt tatgtggaat aagcggtatg
     5521 cagaatgtat tccttttgag aaaacacttg aactaatgga ctgataaatt ccagtttttc
     5581 cacatgaaaa gaacgcatgg cgggcactgt gttggtgaaa tttgcggcgg attgactgtg
     5641 ttggggctgt gtggtataat gaaggtgcgg taaatcggaa tttgtgaagg aggagtatgc
     5701 tatgaatata gggttttggc tatgtggagt tttggttatt ccatttgtga ttataggagt
     5761 gttatttgca atatttaaag aaagagcggc taaatttgtt tcgggattta attcgttacc
     5821 gaaagaagaa caggcattgt atgataaagc tcatatttct cgtgatataa aaaatcagtg
     5881 ttttacatgg gctggcataa tgttagtagg agcagtgttg tcttattttt taacaccata
     5941 tatggctatt ccggctttta ttatttggct cgttttattt ttcaaagagg ttcactttga
     6001 cacacacaaa gcatttgaaa aatatttatt aaaataaatt ccagtttctc cacatgagaa
     6061 gaacgcatag cgggcactgt gccagtgaaa tttacggcgg attgactaag ttagaattgt
     6121 gtggtataat ggaaatgcgg taaatcggaa gttgtatgaa actttatagt gataatcgga
     6181 ggaaatgaga tgctgacaca catcggaaca caaacaatag aaacagaaag actgatactt
     6241 cgtcgttttg agtattctga tattgattct atgctacgca actggatagc agatgaacag
     6301 acacaatggg attatggtga accatattat cctacatctg atgatgttca gaagttattt
     6361 gatacgaagt atattgtttc ttattcctgt aaagattatt atcgctgggc agttattgaa
     6421 aagacatcca gcgagtgtat aggacaaatt gcatttttct tagttgacag caacaatcag
     6481 catggtgaga ttgagtatgt tatcggtcct gcatttcagg gtaagggata tgccacagaa
     6541 atgacgaaag cagttattcg ttttggtttc gaaaaaatca actttcatag aattgagatt
     6601 gattgtcgta cagtaaatga agcttcacgc agagttattg aaaagtgcgg acttacatac
     6661 gaaggaacat ttcgtgattt tttctggaga aaagatcatt ttgagggaag aagagtttat
     6721 tcagttctca aaagcgaatg ggaaaagtta aacaagaaat aaaaattcca gtttttccac
     6781 atgagaagaa cgcatagcgg gcaactgtgt cggtgaaatt tacggcggat tgactgcgtt
     6841 ggggctgtgt ggtataatag aaatgcggta aatcgggatt tgacggaggt aatgaaatgt
     6901 ttgaagaact aggaattagt tttggcacag cactaatcgt cttacttgca ttatatttta
     6961 taat
//
LOCUS       DAWBGR010000135         6945 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2827_length_6945_cov_3.483019, whole genome shotgun sequence.
ACCESSION   DAWBGR010000135 DAWBGR010000000
VERSION     DAWBGR010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2827_length_6945_cov_3.483019"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1787 a         1560 c         1610 g         1988 t
ORIGIN      
        1 atagatcgtg tcagacgtat caatttcatt attctggtcg acgtcggcat ttcgctttgc
       61 ggttttgttc agtttcacag ctccggtaag gcatttgttc agtaatacgc agtctgtgat
      121 gtcgattttg ccgtccagat taacatcgcc gtacagtgtt tccagtgtgt tgttttccga
      181 aagtccatag ggatccagcg tgtagaatgt caatgcagta ttattggaag cataccactg
      241 tacgcattga ttgctggtgt tgaaaatggg ctgacagtcg gataaccgat agtttaaggt
      301 ttctacttta gatgtcgcag ctccttcact attgatcgta accgctttga cagtgatgtt
      361 atgctcactg tcaacttctt cccacatcac aaggaactgt tcttcattga gggctaccaa
      421 ttgcggtgtc cgtacactga tgccgtcctt ttctgtatgc tttgtaagaa agatggtgct
      481 tttgacatcg agatctttgg agagtatggt gacaaagacg tttcgctgtc ctgatgcaga
      541 atagttattc tcgtctgtca tatctacaga atttccggca atgatgcagt tttgttttga
      601 aagggcaaat ccgccgacag aaacaccggt ggcattgtcc ccgatcccgc caagaatgga
      661 ataggcataa ttgtatcgga catctgtgat ttttccgtcc acatcacatt tcgtgattgc
      721 aacggcacga ggatttgcat cgccgtggtc cacccgatag accgattttc cgtcagtaca
      781 gataaactgg ttgaaagaat ggctgacata acctgcctgt gcgaggttca tgacatcgga
      841 ataggaatct aaaactttca gtgtattttc atcaagaaca aatgtcatgt ttgcctgatg
      901 attcagtccg tcactggaag tgtacatttc atggcaggtg tggatgtaaa gctttccggc
      961 agtttccgtc attcgcagag aaccggcatc gaatggaata tacgtgtttt ctccatagat
     1021 cgatttggaa tctaattttt cccagttctt agagtatttt acaacacgca ttatttctac
     1081 gctgtcatca ttatcaggat tggactgccc atagactaaa aagttgtagt cgctgccgct
     1141 gtaataaccg ccgaacagtt ttaactcatc agaaatcgtc tgggaatcca acagctggaa
     1201 atctgcggaa tagtgttcga tgacaaagcc tttattgcga atatgttctc ctcgcgtcag
     1261 tgtaccgtcc tcatttttgg tgagatagga attgacagtg ctggaccagc cgctgtaatt
     1321 gttggcggag agattgatgc tggaagcacc cgttgtagtt ttgatgccgt ctgccgcact
     1381 ttttgtgtgc ggtaatacag taattgtaat tgtcgcagaa agcaggccgt ttttgctggt
     1441 gcaggtgatt tctgcggtac cctcatgcca tgcagagatc gtgccgtttt cattgacgct
     1501 gacaacactg ctgtcactgc ttttccagat atattttgcc gaagaagaaa catcagaaac
     1561 tgcggtgagt gtttccgtgt cgccggcgtt cagcgttgtt ttttcagcag aaatggaaag
     1621 ttctgttgca gatctccagt ccatgaattc gagagaatag gtgttgtttt ctttttctac
     1681 gacaacgaca acattatcca ttttcagtaa gttgaaaaca ttgttttgcg ttgtgtttcg
     1741 ggcgagttct gcaccgtcct tcaggcggta ttccaccagg gtattgttat tggcatagac
     1801 gatcagacta tcgttggcgg cattgtagac agtccgcaca ccaatactgt tgtaatcgga
     1861 gtaactgctg gaatcatctt cgacaggacg cgacacggaa agaactgtat ccatgctctt
     1921 gccgttgaac tgataggcat agagcatgcc taccagtaca ctttgtgcga caatgtattt
     1981 gtcattcaga acttccgcag aacgctgatg attcatatag gcatcttggc agagatacag
     2041 aaggcaatcc atatcccgct gaatcagacc gctttccata aaggaattgg aagaatccag
     2101 cagatacagt ttgttctctt ctacctttcc cattgtcagt ccttttccgg ggtgatgata
     2161 gccccatgaa taatagtcat aatagctttc catgtaaaag gtgccgttct gactttcaaa
     2221 accgtcaaag ttatagattt tcacgctggg atcgacctgt gaaaggacat cgccctcagg
     2281 gcttagcaga tagattcgat atttagaggt tttatccgta ttgtatcctg cgaaataaat
     2341 gcgtccctgt gcatccgcac cgatcgctgt accagaatat ccggataccc aaatttgtcg
     2401 gagcaggctc tgcgtttgca gatcgtagac agagatccgg ctctgagaaa ctacatagag
     2461 catatcgccg gtagtgtaag cgtttgtgca gtcagaaaaa ttgtagactt gttcataggt
     2521 ctgggtcacc agagagtaga agaacagcga acttccctgt aaaaagtaaa tgccgtggag
     2581 cttttcagag gggatctgca tcgttgtagt agaaatgctg gtgtaattta aaatcgtctg
     2641 atccagctcc agtgttccgc tggtgggcat ggtgaaacgt tctccggtta cagtgacagc
     2701 aatagaatca gtatattttc cgtcgccggc agatgcagaa atttctgcgg aacccggtgc
     2761 cactgcaatg atgcgtccgg tattggacac tacaacagta ttcggatcgg aggattccca
     2821 cgtaatgtca tctgcaagaa gcggattcag gaatgcagtg accgatgcat tttctcccac
     2881 atgaagggtc gtgctttctg ctgtgagttt caatgtgttg tcaaaggtat ccagatcgtg
     2941 ccagttgaca cggtaactgg aattgtccat taattttttc aatgaatccc agtcactgga
     3001 agaggaaaga tgggcataga tgtgcagatc gtttgtacca aggcagctgg tagaatataa
     3061 tgatggtgca tttccctgaa agatcatcgt tttcagagaa cgacagttat aaaagctgta
     3121 agaatcgatc atggtcaatc cagccggcag aatcactttc tgaactttct ggttgttgtc
     3181 cattgcttcg cttgcgatct gcgtaaccgt ttcgggcact gtcagtacgg tttgattcca
     3241 tgcggacggc acgaccaaca ggctgctttt ctttttgtta tagagaatgc cgtcgacaga
     3301 agcgtaactg gggttatctg catcaacaac gaaattttcc agaccattgc aggcatacaa
     3361 ataagaaacg ttctcacagt ttgtcaatga agccggaaat tgcagggttc gcagggcatt
     3421 gcagtttcta aaagcagaac cgcttaaaga ttctgttccc tccggaatct gaatgtcggt
     3481 caaatgactg caaccgtaaa atgcatttga attgatcgca tacagggtgt ccggaagtga
     3541 aacactcgtc agacgattgc agccataaaa cgcgttttcg ccaatgctgg taacgccgtt
     3601 ttctacaaca acagagcgga tttgattggc ataggtcaaa aatggcgtaa ctgctgtagt
     3661 ttcatcgtca gaccagtgag aagacacata gtcgtacata gcaccaatac cagaaatggt
     3721 caatgtaccg gtggcagcat ccagcgacca tgtcagttca tctccgcaga atcggtttgc
     3781 tgtatttgcg acaaaagaaa aattttctgt tgcagcatat gtttctgcgg tgcttccggt
     3841 actgccaatg atcgtaaaac caccgatgct ataaaaatcg ccataatcgc tcacataata
     3901 gccaacggcg tgatctccga tataagtaac actttccgga atttccaatg ataccataga
     3961 ttcacagccc agtattgcat aatactcaat gcgttccaga gaatccggaa gcgtaatgga
     4021 acaaatatct gtattgaaac taaagcactc cggagagatc gaaaccgttc cttccggtac
     4081 cacaacatct tctgcggagc cgcgatattt gtaaagacag ctgccgatca tgaccagatc
     4141 gtctgaaatg gcattcagat aagctgttcc gtcaaaggct ccccgtccca cactacgcag
     4201 agaatccgtt cctgtgacag atgccagatc tgtgcagttc cggaatgcgg catcgcctac
     4261 ataaacagtc tgattgagat ccaatgaagt cagagcagat ttgcaggcag aagcagtgat
     4321 tacagtgaca ccgtcaggta cggtaaagtc tgtttctgtt ccatcaatcg catacaaaat
     4381 ataaccgccc agtatgatcg tatctgcatc aattgcattc agcatggcgg ttccggaaaa
     4441 ggcatcttca tcgacacggt ggatgttcgg accaaattga acactttcca acatggtaca
     4501 gccgttgaat gcatcttccc gaatcgtttc caacgtttcc ggtaatgtga tacttgtcaa
     4561 ggagctgcag tttgtaaatg cataggtgcc gatctctgtc agatgctccg gcagattgac
     4621 acttgtcagc tgagggcaat cccagaatgt gccgtacggc agcgtggtca tctgttccgg
     4681 aacatgagca ctggtcagtg ctttacagcc atcgaaaata ctgtatccca ttttggtgat
     4741 ggtgtttgga agatcaactt gctgtaaact ttcgcagccg tagaatgttc tgtcgccgat
     4801 tcgggtaatg gaatccggaa tggtaacggt tttgagtttt tccaattcgc aaaaaatgta
     4861 atcgccgata gaagtgatcc catcactgat aacaacttcc tgaatgacat ctttatactc
     4921 ataaaatggc attgaagaat aatatgaagt agcatcctga atttctccgg agcctgtcag
     4981 tgtcagaaga ccgctttgtg tatccagtgt ccacgagata ttctctccat aagtgccgga
     5041 ataagaatcc tcccgtgtcg tggtatcatc attctgtaaa gggatctctt ctgaagtcag
     5101 accagattct gtttctccgg catcatctgt gtcgtccgtc agagcatcgt aaaagtctgg
     5161 tgtgtgcttg tcaatgggaa tctgatcgcc gttagtgtct gtttcgggtt ctgttggttc
     5221 cggagaagct tcttctgtgg agctctccgg aactgtttct gctgcggtcg gtgcagatgc
     5281 ggtgtctgac ggcacagcat cttcggctgc cgctgggaaa gctgacaatg ttccggaagt
     5341 cagcacgccc gccagaagca gtgcagcagc ttttttccaa tgcaaattca tgtaaacatt
     5401 cctccgtttc ttttgcagca ggttcttagg aaaacgccta acggctttat tcagtattgc
     5461 cttagagttc aagagattcc tgcatacaat tctttgaaag caatcagtca taaattattg
     5521 tgattgtttc caattttatt ttaccataat tttcgtatta agtcaacaaa aaaatccagc
     5581 attctgaaga aatgattttc ctgagatcaa ataaatattg gtaagcttaa aattaacaag
     5641 aaatatatct tttgctggca ataaaaaatc tgccctgcac gccatacaag acagattttt
     5701 ccctgaaaac aaaaatatcc ttacagcttt gcagccagtt ctctctgcac actctcgtcc
     5761 ggaatatcga caatgccgta atccttttct ttataattcc aatacgcacg accaatgccg
     5821 tatttatcga acactgcaca gatgtctgtt gcccagcgga tcttggaatc gttgtccgcc
     5881 agatcgatca cgccgtattc gccgcagtac agcggaatgt cccgttcctc tgcaatgtcc
     5941 agtgccggac ggaacaaggt ttcaaagaag tccggtccca tttccggcag ttcgctctcg
     6001 aaaattgctc cggcgagctc ctgcgaaagc attttgcttg cttcccgata attttcaatg
     6061 gtgttgggat aggagatccg gaaatccgac ggcataccct ttacccaata tgcaccctga
     6121 tgtgtaaaga tcagcggctc atagctgtgg aacgtatagg caaccttagg gtcggtgacc
     6181 tctgccagca tcggcacgct cagcacgtta ttgtacatga caccgccaaa aatgatccag
     6241 ctttccggtg caaactttcg gatctctgca atggcatgca gggcgatgcc gttccacgcc
     6301 tccgccacct ccggttcaat gatctcgttc agcagttcga atgccaccag ttccggttcg
     6361 ccgccgtagc gtgctgcgag gattttccac aatgtgtaaa accgttcctg caatgccgtg
     6421 tcatagaaaa ataccgtctt gtcggtttcg tccagcgggt caaaggaata gccaaaggtt
     6481 ttgtgcagat ccaggatcat acggattccg tgggttctgc accaacgcag gcagttgtcc
     6541 agatagagat agccctctga cttcggggtg ccgtcttctt cttccagcag tacgtagtcc
     6601 accggtacgc ggacgtgatc cagtcccagc gatgcaatgt aggcaatatc cttttcggtg
     6661 atgaatgttt cgtagtgttc cttagaagtg tcactgttct gggacagcca gccgcccaga
     6721 ttgacaccgt ggagataatg ttctaatttt cgcatgattt ttcttccttt ctgtgattgc
     6781 tttttgagtg ttttgcattt ggtgatttca ttatacggat tttttgcagt gccgtatatt
     6841 gtttttgttg catttttcgc agaaatgttg tataataaga gaaatgctga ataaaggaaa
     6901 cactgaataa agcttgtgcg taagatgcgt agtcagattt ttcgg
//
LOCUS       DAWBGR010000136         6942 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2828_length_6942_cov_4.474662, whole genome shotgun sequence.
ACCESSION   DAWBGR010000136 DAWBGR010000000
VERSION     DAWBGR010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6942)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2828_length_6942_cov_4.474662"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1484 a         2004 c         1812 g         1642 t
ORIGIN      
        1 gttggcaagc cccaggcgga cagcgaggta cgagcgaatt ccgccggacg gtttcaacac
       61 ggctgtcctt aatccagtat ttcgtaactt tttatatcgt tttcggtaaa agtaatagaa
      121 ctgccatcgt caatgtcaaa acatattgcg tctacttcct cgtcattttc gctcttacct
      181 ttccattccg attgccgcat caaccgcccg cagttcttcc tccgtcagcg gttcctgtcc
      241 gacgatctgg agcgtatcga taagctgttc cggacgggat gcacctacca gcacactggt
      301 cactgccttt tgccgcaaaa tccagcagag tgccagctgt gccagcgtct gtcccctgcc
      361 ctgtgcaatt gcctgcagct gccgcagctg ggagagccgt tcttccgtca gctgggattc
      421 ctgcaggaat ctgccgtcag tccgaatccg gctgtcctcc ggaatgccgt ggagataccg
      481 gtctgtcagc atgccctgtg ccagtggact gtagatgatc acaccctttt gcagctgctc
      541 actcgctgac aaaatgccgt ttttctccga aacacgatcg agaatggagt accggttctg
      601 gttgatgatg aacgggcagt gcagctgttc cagaatcgcc gccgcacggc tcatggtttc
      661 cccgtcatag ttggacaagc ccacgtacaa tgccttgccg ctttgcactg cctgtgccag
      721 tgctcccatg gtttcctcca gcggtgtttc cggatccgga cggtggtgat agaaaatgtc
      781 cacatagtcc agccccagcc gctgcaaact ctgatccaga cttgccagca gatatttccg
      841 gctgccccat tcaccatagg ggccttccca catgtcatag ccagccttgg tgctgatgac
      901 cagttcgtca cgatgtccgg caaatatcgt tttcagcagc ttgccgaagt tccgttccgc
      961 agcacctgcc ggcggaccgt agttgtttgc caaatcaaag tgcgtgatgc cgccgtcaaa
     1021 tgcggtttca cagagctgtg ccatgtgctc cagactgttg gtatcgccaa agttatgcca
     1081 cagtcccatc gaaagcagcg gcagtttcag accgcttctg ccgcaccggc ggtattccat
     1141 ttgggtatag cgttctgcat tcggaatgta tgccattttt tactcctcct tggaattctc
     1201 gttctcatct gagagatagg tttctacaat gtatttcggg cgttccttgg tttccagata
     1261 cacctttccg atgtactgcc cgatcacgcc gatcgccaga agctgtaacc cgccgatcgc
     1321 ccagatcgac acgatcagcg aagtccagcc ggtagtagtg gctcccataa agtgccgcac
     1381 tacagaatag atcagcatgc cgatactcac cagaaagatc agcatgccca gacgggtgat
     1441 caaagtaata ggctttgtgg aaagggacgt gatcccttcc cacgcaaagg agagcatctt
     1501 tttcaaagga tacttggacg taccggcaag gcgttccttg cgggcatagt acaccttatc
     1561 gctggggtag ccgatcagcg gcaccacacc gcggagaaac atattcactt ccttgaacga
     1621 ctccagcccg tccagtgccc gccggctcat cagccgatag tcagcatggt tgaacaccag
     1681 atctgctccc atgtgcttca tcactttata aaagccctcc gcagtacact ttttgaacag
     1741 ggtgtccgtc tttcgggcac tgcggacacc gtagacaatg tcgtttccgg cttcatactt
     1801 tgcaatcatc tgatcgatcg catcaatatc gtcctgcaaa tccgcgtcca gcgaaatggt
     1861 catgtcgcag tgtttcttca ccgtcatcag ccctgccagc agagcgttct ggtgtccgcg
     1921 gttgcgggaa agggaaattc cctgatagat cggattttcc gcgtgaaact tctggatcag
     1981 ttcccatgtc cggtctttgg agccgtcatt cacaaacacg atccggctct tttccgttat
     2041 ttttccggca gtcatcagcg actgcatttt ttccagcagc tgccttgcgg tttccggcag
     2101 cacttcttcc tcattatagc aaggaatgac caagtatagc gtatccgttt tgtgtacctc
     2161 cctgcgtgat tcagcgtttc ttaattgtat catattttcg gcaaaatgtc aaacaaaaaa
     2221 gcactccgga gaagtttccc cgaagtgccc tttggataaa atgattttac aatccgaaat
     2281 atgctgcgat cacagctgct tctgcatctg ccagccgctg taggttggta tcactgagca
     2341 gccagttggt cgcccgcaaa ttggtgtggt aactgtgctc cagcaagatc gccggtgttc
     2401 ccacagaagt tgccccgcgg agcactccgt agtagtcgcc gttgttgccc acacgcttcc
     2461 agatcgtacc accctgtgca gtgctcatta cctgatgcac ggtatttgcc agctgctgtc
     2521 ccagcacgtc catggtgccg gtcacggtac agcatgccag cggagcatct acggaactgt
     2581 tgccgcaggc gttgctgtgc aggctcagga acaggtcaca gcctgcggag gtttttccgc
     2641 gggtttccag tcccagatcc cctgcctgtg tcggtctggt ggtaatgact tccatgcctc
     2701 tggcttccag agccgatttc agcttcagat gcagccgcca gctcatatca gactcataat
     2761 aagctccgtt taccggactg cggttatact tgccgtagtg tcctgcgtcc agacaaatct
     2821 tgattttttt ctgtgtaccg tcggcattga accgatactt cttgccgtca atgacttttt
     2881 cgccggtcac cattgtgccg tctgcgttga aataatattt ctgaccgccg aaagtctgcc
     2941 agccggtttt catgacaccg tctgtgccga agtagtactt cttgccgccg acattctgga
     3001 aaccggtctg catggaaccg tctgtggcaa agtagtattt tttgccgtct accgtctgca
     3061 tgccggtctg tgcaatacca tcgtttccaa gataatagtt cttgccgctg atattctgca
     3121 tgccagtctg catggtgtaa ctgtcattga agtaatagcg gctgccatga tcctccaccc
     3181 agccggacac cggagcaccg ttctgatcgt aatacgatat cgtattatcg gagaacttgc
     3241 aaaatcccag cttctgggta ccagtttcgc tgtcgctttg atagcgtacc ccgtctacgg
     3301 tgaattctcc ggttgtcttt ccggaatccg cactgacata gtagcggtaa tcgccgtagc
     3361 tgacccagcc gctgtccggc tttccggttt ccggatcgta gtaccgccgc acaccgtcta
     3421 ccgtccgcca gccggttttc tgtgccccgc tgtagtcgaa caaatacgct gtgccgtcga
     3481 tctgctgggc accgactgct gcctcataag tcacgctgtc gtagtagtgc catgtttcgt
     3541 cgattttctg ccagccggtc agctgtgtac cgctggaatc gaacaagtac agcacgccgt
     3601 ccactgcggt ggcatgtacg gcaaacgaac cgtcgctgta ctgatacttc cggcagccgt
     3661 cctctatgat ccagccgccg gtaatttccg gtgtttctgt ctgtggctgg aactccggac
     3721 cagttgccgt tgccaccact gccgaagcgg tttctgcggt ttgcagaacc tccggcaaag
     3781 ccgtttctgc cgaagctgtc acaccgccga acaacagcat gccgcctgcc gcagcaccca
     3841 ttgccaacag ggacagccat ttgggataat gcattttcaa acctcttttc taaattggaa
     3901 aaacacttta aggataataa ttatccagac attcccagag agaatttcaa aaagagctcc
     3961 ctctttttat cgatttcaaa ccgatatttt atattataca tggattcggc acaattgtca
     4021 aggggtttct tgtgattttc acaaaaagct ttcctatgtg gtcatgcttt ttagcttttc
     4081 cgcatatagt agccacaaac aaaaacatca tttgggaaga gtttggacag cactgaacaa
     4141 agctttattc agtgtttccc ttgcaaaaga aagaaggcat aacatgaacg gtctgacaac
     4201 caaaaccgca cgggaacgac tgaaacagga cggcgaaaac gaactggcgg cggcagcgaa
     4261 acgccgtccc ctacgcatgt ttctcgggca gttccgagat gtaatggtat tgattctgct
     4321 ggcagccaca ggcatttctg cgtttctggg ggagatcacc gatgcggtca ccattatcct
     4381 cattgtcctg ctcaacgcga ttttgggctt tttgcaggaa taccgcaccg agcagacgct
     4441 ggaatctctc cgcaatctga cggctcccac cgccacggcg tgtcgggacg gcaagtggca
     4501 gaccattccg gcacgggaac tggtctgcgg tgacttggtg cggctggaag ccggcgacaa
     4561 tgttccggcg gacagtgcac tgatacaggc ggcggggctg gcggtgaacg agtccattct
     4621 caccggcgaa tccgaagcag tttccaaaca ggcaggcgac cctgccgaca cccgaaacga
     4681 tctgcataaa cacaacatcg tctacgccgg cacggcaatt ctgcggggca gtgccgaagc
     4741 agtggtcatt gccaccggca cacgcaccca aatgggacag atctccacca tgctgcaaga
     4801 ggtccggcag gaaatgacac cgcttcagaa acgccttgcc ggactgggaa aagtcgtggc
     4861 tctgctgtgc cttggcgtat gcggagccgt gttccttgcc ggtgtgctgc ggggcgagcc
     4921 ggtttttgac atgctcatga ccggcattac cattgccatt gctgccattc cggaggggct
     4981 gcctgccacc gtcaccattg cccttgctct ggcagtcagc cgcatgatga agcacggggc
     5041 actggtgaac cggctgcaca gtgtggaaac gctgggctgt gccagcgtga tctgctccga
     5101 caagaccgga acgatcaccg agaaccgcat gactgtcact gccattgttg ccggcggcga
     5161 acgcttttct gtcacgggga ctggtctgca aaaagccggt gccattcagc tggacggcag
     5221 caacatcaat cctctctcca agccggcact gcgggaactg ctcacctgcg gcagtctttg
     5281 cagcaccgca gagatccaca gcctgcagga aaagcaatcc cgcaaccgcg gcagccgcac
     5341 ggaaaagggc acatggtctg ccaccggtga ccccacggaa acggcattgc tgatcgccgc
     5401 ggaaaaaggc ggcatttcca gaaaagccct gctccgcacg caccctgtcc agcacatgga
     5461 gccgtttaac agtgaaaccc gccgcatggc ggtaacggtg acagacggag ccggcacctg
     5521 cacctattgg aaaggagctg ctgatgtcat tttgcccctg tgcggattcc aaatgcagga
     5581 cggcaaaagc gttccccttt cggagggtgc acgagccgct gtccgcaaac aggtcgccgc
     5641 catgtcagac gaagccctgc gggtgctggc gttcgccaaa caggaaggca gtgcctgcat
     5701 ctttctgggg cttgccggca tgctggatcc gcccagagag tccgcacgca cggcgatccg
     5761 cacctgtgcc aaggcgaata tccgcaccgt catgattacc ggtgaccaca agaacactgc
     5821 cgctgccatt gccagacagg caggactgct ccgcggaaaa aaagccatga ccggagccga
     5881 actggatacc ctgactgatg cccagctaga ccggtgcatt gaccagtatt ccgtctttgc
     5941 ccgtgtcaat ccggcacaca agctgcggct ggttcgagct tacaagcggc gtggagagat
     6001 cgttgccatg accggcgacg gcgtcaacga tgcaccggcg atcaaggaag ccgatgtcgg
     6061 cgtggcaatg ggcaaaagcg gcaccgatgt ggcacgacag gcagcagacg tggtgctgac
     6121 cgatgacaat ctggcaacac tggtggacgc agtggaacag ggacggtgcg tctacgccaa
     6181 tatccgaaaa ttcgtccggt atctgctgtc ctgcaacatc ggcgaagtgc tgaccatgtt
     6241 tctgggaatc ctcatgggca tgccggtggt gctgctcccc acccagctgc tgctggtaaa
     6301 tctggtaact gacgggctgc cggcgattgc tctggggctg gagccgccgg agccggaagc
     6361 catgcagaag ccgccccgca ggcctgacga aagcttcttc tctgacgggc ttatgggacg
     6421 cattttcttc cgcggcatgc tcatcggtat ctgcacactg ggagcatttt ccgctgttgt
     6481 ccgcaccggc ggcacactgg aagccgcacg gacagcggca ttgtgcaccc tgatcctgtc
     6541 ccagctggta catgttttcg agtgcaaatc cgaacggcgt accctgttct ccatgcccta
     6601 cggcaacaat ctgtggctgg tgggagccgt gctggtttct gtggcagtgc tggcggcggc
     6661 agttgccgtg cccatgctcc gcgtgatctt ctccacagtc atgctgaccc gtccgcaatg
     6721 gtacatcgcc ctgggcttct ccctgactgt gcctctgtgc agttccattg ctggattgtt
     6781 ccggcggaaa aaacgggact gacgattgcc atacaaaaaa acggcgtatc cgaaattgta
     6841 ctcggatacg ccgttttgta taatgggaaa attcaaaatg tcatttgttt agggaaacac
     6901 tgaataaagc ttgtgcgtaa gatgcgtagt cagatttttc gg
//
LOCUS       DAWBGR010000137         6885 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2845_length_6885_cov_4.392533, whole genome shotgun sequence.
ACCESSION   DAWBGR010000137 DAWBGR010000000
VERSION     DAWBGR010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6885)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2845_length_6885_cov_4.392533"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1918 a         1727 c         1809 g         1431 t
ORIGIN      
        1 gcagattgcg tgcaaagccg tcaggcgggc tttatgcagt gtttccttta ttatatcggt
       61 ttttgttgag tttgtcaagg gcgatttttg aaaagctacg gaaatccatt ttcatgacag
      121 cctctccgcc gccggcttca ccgtttctcc gttcctttca gggcggctct gaagaatgaa
      181 aatggattca tgcggtgttg tccaaaattc atagttgaca actgcacgaa aatatactat
      241 aataatataa tgtctaagct ggcaggcagt tggtctgcgg tagaaagaac atggcggcag
      301 gcggtctgca cagacacact gccctgccgc acggaatcaa aggagcatga gaattatggc
      361 aaagaagcag atttcaaaat ccggtgcagc caagctgcgg gaagaactgg actatctggt
      421 aaccgtccgt cgtgcagaaa tggcacagaa gctgaaagaa gcacgtgcac agggcgacct
      481 ttccgaaaat gcggaatatg acgaagcgaa aaacgaacag gcgatcatgg aagcacagat
      541 catggagatc cagtacacac tggacaactc tgagatcgta gacgacaacg cttctatcga
      601 tgaagtcggt ctgggttcta ccattaagat caagaacttc cgcaccggca agatcgaaac
      661 cctccagatc gtcagcagca acgaagccaa cttcaaggaa ggcaaaattt ctgacgaatc
      721 tcctatcggc aagggtgcaa tgaaaaagaa agtcggcgac aacttcctca tcgaagcacc
      781 ggcaggcgaa ctgaagttcg agattctgga aatcagcaag taatagatat aggagtgaat
      841 cccatggcaa atcagaacac accgtccgtt ccggaacagg aacaggacag caacaccctg
      901 cggcagatcc gtctggaaaa gctgcggcag cggcaggcag acggcaacga cccgtttacc
      961 attaccaagt atgatgtcac cgcaaaggca gcatctgtca aggcagacta tgaagtcacc
     1021 gaggcaaagt tcaaggaaga agccggcgat gacgaagaag ctttacaggc aaagctggac
     1081 accctgaaag aacagccgat ctccattgcc ggacgcatta tgagctggcg ggatatggga
     1141 cgtgcaaact tcattgacgt gcgggattct actgaccgca ttcaggtata cgtccgcatg
     1201 aacgacatcg gcaaggaaaa tttcaaggaa ttcaagaagt gggacatcgg cgatattgtc
     1261 ggcgtgaagg gcttcgtatt ccgcacccgc aagggcgaaa tttccattca cgcaacagag
     1321 atcaccatgc tttccaagtc cctgctgccc ctgccggaaa agtggcacgg tctgaaggat
     1381 caggacaccc gttatcgtca gcggtatctg gatctgatcg taaacccgaa tgtcaagcag
     1441 acttttgtca ccagaagcca gatcctgcgg gaaattcgtg cttatctgga cggcatcggc
     1501 tatctggaag tggaaacacc ggtgctgctg cctttgcaga tcgctgctgc ggcaaagccg
     1561 tttgtgacac atcacaacac gctggacatg gacatgttcc tgcgtatcga aaccgaactg
     1621 aatctgaaaa agctgatcgt cggcggtttc gaccgtgtgt atgaagtggg acgcatcttc
     1681 cggaacgagg gcatggatcc ctctcacaat ccggaattta cctctatcga aatgtatcag
     1741 gcatacaccg attatcacgg catgatggat ctgatcgaag atatgtaccg cacactggca
     1801 aagaagatct gcggcaagga catcatttcc tatcagggta cagacattcg tctgggcgaa
     1861 ccgtgggaac gtctgacaat ggcagaagct gtcaagaagt atgccggcgt ggactacaac
     1921 gactgggcaa cggatgccga tgcaagagcg tgtgccaagg aaaagggcgt ggaagtcgaa
     1981 gaaggcgaaa acgctaccaa gggacacgtg ctgatcgcgt tctttgacgc atttgtggaa
     2041 gaacagctga tccagccgac tatcatctat gattatccgg tagaaaactc cccgctggca
     2101 aagcggaagc cggaaaatcc ggcgttcaca gagcgtttcg agtacttcat ctatgcccgc
     2161 gaaatgggta acgcgttctc cgaactgaac gatccggtag accagaagga acgttttgtc
     2221 aagcaggtcg aagctcgccg tgcactgggt gatatgaccg gcgaagtaga tgaagatttc
     2281 gtcacagctc tggaatacgg catgccgccg acaggcggtc tgggactggg tctggacaga
     2341 ctggtcatgc tcctgactga cagcccgtct atccgtgatg tcctgctgtt ccccaccatg
     2401 cgaccgctgc cgaagctggg acaggaacag gacgaagcag acgccgggga agaagaaccc
     2461 gaagcataat aatttcattt ctgacaggca gatgcctttg ggcaataccg cacaaagagc
     2521 gtctggcgac tttgaaatga aatttccaaa cacatgaaac gataaggggg aattcgtaca
     2581 gatcgagggt atctgtggga gtttccccct tttgattttc tcaaaaagtg aggtcacttg
     2641 atgcaggaag aagaaaaaca aaaccattct gccggcacgc cgccggaaca gccgaaaaag
     2701 cacaaaaagg aaaaaggcaa atctctcctc gaaaccatgc gggaaatcga tgccaaagaa
     2761 gccgaaaaag aggcggaggc ggaagaacgc cggcaggcac tcctcgcgga acgggagaaa
     2821 aaggaaaaag aggaatacgc caagaagatc cagcaggacc gcattgaatt gatgcggctg
     2881 aaacagggca ttatcaccga gagcgacacc atctacgagg aaaaagagga caagcccaag
     2941 atgtccttct ggaagaaact cggcaatttc ctgtaccaca gcaagtggtg gctgggaatc
     3001 accgtgttta tcgtaggcgt tttcgtgttc ctgatcgtgg actatgtgac gaaagtccgt
     3061 ccggatatga tcgtactgct gattaccgac gacacggaaa tgcagaatca ccgccagcaa
     3121 ctagaagaat atctggagca gttcaccgac gatgaaaacg gggacggcaa ggtgcatgtg
     3181 gacatatacc ccattcccgt cagcgacaac attgacgaca tggactattt caccggcaat
     3241 tccaccaagc tttccgcgga attccagatg ggcgaatcgg tcatggtgat tactgatgcc
     3301 aaggcaaacg aatacatcat ggcagacgaa accctgacag accttagcga aaaatacacg
     3361 gggcacgaaa atatccgcgg caacggctat tacctgcggc acacggattt cgccacgaaa
     3421 atcgactatc cgggaaatgt agaccgcgac ctttccattg gtctgcgtgc accggtaaaa
     3481 acttcggaca gcaaggaaaa gatgcagaaa acctatgacg tggcagaaaa ggtatttctc
     3541 cgcgttatgg acgatctgga caacaccacg gaaccggagg acattgtcac caccgaacct
     3601 gccgaaacgg cagttactac caccaaggag gactgaatga tgctacgaat cggaaaccac
     3661 ctgccctcgt ccaagggcta tctggcaatg ggaaaacatg ctgtcaagct gggggcaacc
     3721 acgtttgcct ttttcacccg caaccccaga ggcggcacgg caaagcccat tgcggaaagc
     3781 gatgtacagg cgtttctcgc ctttgccaag gaacacgaca ttgaccattt ggtagcacac
     3841 gctccctata ccatgaacct ctgttccgcc aagccggaca ttcggcagtt cgggctgaac
     3901 atgttacagg acgacatgaa acgcatggag tatacgccgc accagtacta caacttccat
     3961 cctggcagcc acaccggaca gggtgcagaa gtcggcattg aacagatcgc cgatgccctg
     4021 aaccagacgc tgacaccgga gcagaccacc actgtcctgc tggaaaccat ggcgggaaag
     4081 ggcagcgaag tggggcgttc ctttgaggag ctgcgtgcca ttctcgaccg cgtccagctg
     4141 accgaaaaaa tgggcgtttg tctggacacc tgccacatct gggacgcggg ctataatatt
     4201 gcggaagatc tggacggcgt gttgacccag tttgacaaag tcattgggct ggacaagctg
     4261 tacgctgtgc acctgaacga cagtatgaat ccctgcggtg cccacaaaga ccggcaccag
     4321 aagctgggac agggctatat tggactggaa gcactggtgc gggtgatcaa ccaccctgcc
     4381 ctgaaacatc tgcctttcat tctggaaacg cctaacgagg acgacggcta tgccgcagag
     4441 atcgccttga tgcgggagcg tgcggaatga gtgcggaaag caaacggaaa tggggtgcac
     4501 tggcggcggc tttggtatta ctgctgggcg gtggcacagg cggattcttt ctgggaaaag
     4561 cccagactct gaaacaacag cagcaagtca ttgacgtgca ggactgcacc accatttatg
     4621 caacaatttc tcagatcagc ggcggaaagg ttcgtgctga cggactggaa gtcaacgaca
     4681 tcaacggacg gggcagcttt tcgtttaccc tgtccgagga tacgccgatc acatggcacc
     4741 acaccaagat cgccctctct gccctgcaaa aaggggacac cattgcgatc acctacaccg
     4801 gtgacacaaa ggaaacctat ccggtgcagc tggaacaggt agtcaaggtg gaagtgctga
     4861 acgataccat atagacagac tggctggacg ttccgcatag ataccgcatg aaatcctgtt
     4921 gatttctgta caacaccgac aaattgcgac gtgtttttgt ggaaatattt tctcattttt
     4981 tgcgaaatct cttgacgaat cgcaaaaaaa agagtataat aaaagtacag gcgacacggt
     5041 acaccgattg tgtgcaggat acattctcag atgttgatgc atatgctgaa cagtctgcac
     5101 acagactgtg gcacaaagcc ggcaaacagg cttgtgcggt gttgcctatg atacatgcga
     5161 aaggagtatg tgccctgtgg cacagtagat tgcaagatga aaaatatttt atttgcggct
     5221 tccgaatgcg ttccctttat caaaaccggc gggcttgccg atgttgtagg tgcactgcct
     5281 aaaagtctga atcacaacga gtatgacgtt cgcgttgtca ttcccaacta cacctgtatc
     5341 ccgtgggaat accgcagcca gttccgctat gtcagccatt tctacatgga ctacggcaga
     5401 catgtggaaa atgtccatgt cggtatcatg gaatatgtct atgagggcgt acacttctac
     5461 ttcatcgaca acgagtatta tttcaagtgt gaccgcccct acagcgactc cacccagtgg
     5521 gatattgaac gcttcatttt cttctccaag gcggttctgg cggcaatgcc tgtcatcgga
     5581 ttccaccccg acatcattca ctgccacgac tggcagacgg gtatgattcc ggtattcctc
     5641 aagagcacat ttgccaccca cagcttctac tggggcacca agagcatcat gaccattcac
     5701 aacctgaaat ttcagggcgt atgggacatt cgtcactttg tctacaacac caacctgccg
     5761 gattacatgt tcacaccaga taagctggag ttcaagagcg atgccaacat gctcaagggc
     5821 ggtctggtct acgcggacta catcacgacc gtcagcgaaa cctatgccga agaaatcaag
     5881 accccctatt acggcgaaca gctggacggt ctgctctgtg cacgctcccg ttcgctccgc
     5941 ggtatcctga acggcattga ctacgaggtc tacaatcccc agaccgacaa gaagatcgat
     6001 gtacactatt ccgtcaagga cgctgtggaa aagaaaaagc aggcaaagct ggctctccag
     6061 aaagaactgg ggctgccgga ggacgagaac aagtttatga tcgcgatcat ttcccgtctg
     6121 accgaccaga aaggtctgga tctggtagac tggatcgccg accgcattac cgacgagttc
     6181 acccagtttg tcatcatcgg caccggtgaa gcacggtatg agaacatgtt ccggtattat
     6241 cagaacaagt atccggatcg ggtttctgcg aacattttct attccgatcc gctggcacac
     6301 aagctgtatg ctgcggcaga tgcgatgctg gttccgtcac ggtttgagcc ctgcggtctg
     6361 acccagttga tctcgctccg ctatggtact gtgcccattg tacgggaaac cggcggactg
     6421 aaagacaccg tcgtacccta taacgaatac gaaaagaccg gcaccggctt cacatttgcc
     6481 cattacaatg cagatgatct gctgtatacc atcaactatg cgaagaagat ctacttcgac
     6541 caccgcgagg attggaatga cattctctgc cgcggcatgg aacaggattt ctcgtggagc
     6601 agttctgcat tgcagtatca gggcatgtac gactggatcc ttcggggcat gcaggattaa
     6661 aagaatatca aaggcaatac cgcacaaaaa atcccctgac agacaagggg catgaaatat
     6721 gtactatata aaagagagga cagaccggtt gacggttgtc ctctcttttg cgtttgaagt
     6781 tttgacttgc ccgaaaatag caagcggcat ttgacattag ggagtgagtg gtatggaata
     6841 cgtaacttgg tcagatttga ttcagtacag tatcctgatc gttgc
//
LOCUS       DAWBGR010000138         6807 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2887_length_6807_cov_3.901363, whole genome shotgun sequence.
ACCESSION   DAWBGR010000138 DAWBGR010000000
VERSION     DAWBGR010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2887_length_6807_cov_3.901363"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1853..1862
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1899 a         1496 c         1741 g         1661 t
ORIGIN      
        1 ggggtggaat agtctggacg acaattccat tgctatgatg tattatcagg caatctccga
       61 aaaatacggc atttctttag atgttcctgt aaaggaactc tccaaggaac agctgaactt
      121 atttttgtac ggcacaaaag aagatccact gtttctgcat cgcaccacag ccggcggcag
      181 aactgaggga ttccgtgctc cgtttgaagg tgtaattccc aatctgaaac gacgattcgc
      241 agacggcggg gaatgggcaa aggaagaaat cagcagtgtg atgagtgatg tagcttgtcc
      301 ggtgtgccac ggcaaacgtt tgaagccgga agtactggcg gttacagttg gtgacaaaaa
      361 cattatcgat ctgactgaac tttccgttac aaaagcactg gacttctttg ctcagctgca
      421 attaacagaa cgggagcaat ttattgccgc acgaatcgta aaggagattc gggaacgact
      481 gggattcctg aataatgtgg gactggaata tctgacactt tcccgcaatg ccggtacgct
      541 ttccggcggc gaaagccaac gtatccgctt ggcaacacaa atcggttcct atctaatggg
      601 cgtactgtat attctggacg aacccagtat tggattgcat cagcgggata acgataaatt
      661 aattgatacg ctgaaacgtc tgcgggattt gggcaatacg ctgatcgtgg tagaacatga
      721 cgatgacacc attctggcag cagactatgt ggtagacatc ggtcccggtg caggaattca
      781 cggaggaaat gttgtctatt ccggtgatgt tcagggattg cttgcctgcg aaaaatcgat
      841 taccggtcaa tatctcagcg ggcagcgaaa aatcccgctt cccaagggac gccgtaccgg
      901 aaacggaaat atgctaacca ttcatggtgc aagagagcat aacttgaaaa acattgatgt
      961 ttccattcct cttggtgtat ttacctgtgt aactggtgtt tccggctctg gaaagtcctc
     1021 gctggtcaac gagattttgt ataaatatct ggcggcaagg ctgaaccgtg caagaacccg
     1081 ccacggagat tttgacagca tggagggaac ggagtttctg gacaaggtca ttgacattga
     1141 ccagtctccg attggacgga cacctcgttc caatccagca acctataccg gtgtatttac
     1201 cgacattcgg gaactgtttg ccaaaaccaa tgatgccaaa gccaagggct ataccgcagg
     1261 acgattctct ttcaatgtca aaggaggcag atgcgaagca tgtcagggcg acggtatcct
     1321 gcgaattgaa atgcactttc tgccagatgt ttacgttccc tgcgaagtct gcaaaggaaa
     1381 acgctacaat cgagaaacgc tggcggtaga gtacaaggga aagtctattt atgatgtact
     1441 ggaaatgaca gtagatgaag gcgtggtatt ctttgaaaat cacccgaaaa ttgcccgaaa
     1501 gttgaaaacg ctgcaggaag taggactggg ctatctgaaa atcggtcagc cggcaaccac
     1561 gctctccggc ggggaagcac agcgtgtcaa acttgcaaca gaactttcca aacgtcccac
     1621 cggaagaacc atttatattc tggacgaacc gactactggc ctgcatactg ccgatgtaca
     1681 ccgtctgatc gaagtacttc agcgtctgac tgatactggg aatactgttg tagtaattga
     1741 acacaatcta aatgttatca aggtggcaga ctggatcgta gatctcggac cagagggagg
     1801 cgaccgcggc ggcaccgtcg tagtcagcgg tacaccggaa caggtagcag ccnnnnnnnn
     1861 nnaaactgta tgaaatgcac caaccgcatt tcagaaaaat cgttaaattt caggctgtat
     1921 cggataatat atgaatttcc ctttcacgat gcagtctatc gcagaaagga tgtcaaacaa
     1981 atggcagagt taaacaaggt atctgtagac gatctgaaag tgaccggcaa acgtgttctg
     2041 gttcgcgtgg acttcaatgt tccgatgaag gacggcgtta tcacaaatga taaccgtatt
     2101 caggcagcac tgccgaccat caacaagctg gtttctgaag gtgcaaaggt aattctgtgc
     2161 tctcatctgg gcaagccgaa gaatggtccg gaggaaaagt tctctctgaa ggctgctgct
     2221 gctcgtctgg ctgaactggt ttccgcaaag gttacttttg ttccgtctga tgttgttgta
     2281 gatgatactg taagaactgc tgtagacaag atgcagaacg gtgagatcgt tgttctggaa
     2341 aacactcgtt tccgcaagga agaaaccaag aacatcgaat ccttcagcaa ggatctggct
     2401 tctatcgctg atgcatttgt tatggacgca ttcggcagtg cacacagagc acactgctcc
     2461 actgtcggcg taacagaatt cgtaaaggaa actgctgtcg gctatctgat gcagaaggag
     2521 atcaagttcc tcggcaatgc tgttgaaaat ccggttcgcc cgttcgttgc aattctgggc
     2581 ggtgcaaagg ttgcagataa gctgaacgtt atctccaacc tgctggaaaa gtgcgacacc
     2641 ctgattatcg gcggcggtat ggcttatacc ttcctgaagg cacagggcta tgaagttggt
     2701 acttctctgg tagatgacac caagatcgac tattgcaagg aaatgctgga aaaggcaaag
     2761 gcaaacggca aggctctgtt gctgccggta gacaccgtaa ctgcttctga attcccgaac
     2821 ccgatcgatg ctgcaatcga agttgttacc tatgattcca acaagattcc ggcagacaga
     2881 atgggtctgg atattggcga aaagacacag gctctgtttg cagatgcagt taagactgca
     2941 aagactgttg tatggaacgg accgatgggc gtattcgaaa acccgattct gaacaagggt
     3001 actgttgctg ttgcacaggc tctggcagat actgatgcga ccaccatcat cggtggcggc
     3061 gactccgcag cagcagtaat gcagctgggc ttcgcagaca agatgagcca catttccact
     3121 ggcggcggtg catctctgga atatctggaa ggcaaggttc tgccgggcgt tgcagcaatc
     3181 gctgacaagg actaatcaaa ataagaacgc agctgttttg tgctgctgca aacaggagga
     3241 ttctgtgaaa atggaaacct ccgtttgtgc tgtcttggga aaggaatgat accatgaaaa
     3301 aatgggaagt gcaggattct gtcaaaaatg cgtttttgaa tccggctgct gcatttgcac
     3361 tgggtgcttt ttgcggtctg gcgattggaa tgctctgtat gcctttttcc aaaggcatcg
     3421 tcatcggcag ctataatgga agcaacaaca aggaatccca cgaagaaggg gattatatta
     3481 cagatgcaga agcatcagaa tcatcagaaa agaggaattt gtcatgaaca aggcactgag
     3541 aaaggcaatt attgccggca actggaagat gaacaagaac cgcaaggaag cagcagagct
     3601 gatcaacgct ctgaagccga tcgcagctga caagacctgt ggtgttatca tttgtgtacc
     3661 gtttaccaat ctggaaaccg cactgtccct gactgagggc acaaatatcg aagttggtgc
     3721 agagaacgta catttcgaaa agagtggtgc attcaccggc gaaatttccg ctgatatgct
     3781 gaccgaaatg ggcgtaaagt atgtcatcgt tggtcactct gaacgtagac agtacttcgg
     3841 cgaaaccgat gaaactgtaa ataagcgtac tactgctgca attgaagctg gcatgaccgt
     3901 tattctgtgc gttggtgaaa cactggcaga acgggaactg ggcatcacag aagaagttgt
     3961 tgcaaagcag gtaaaggttg ctctgcacgg tctgactgca gatcaggtaa agaatatcgt
     4021 tatcgcttat gagccggtat gggcaattgg cactggtaag actgctactg cagaacaggc
     4081 tgaagaagtt tgtgcattta tccgtgcaac tgttgcttct ctgtacgatc aggcaattgc
     4141 aaacggtatg accattcagt acggcggttc tatgaacgaa aagaactgtg cagaactgct
     4201 ggcaaaggaa aacatcgacg gtggtctgat cggcggtgct tctctggttg ctgaaaagtt
     4261 cggcgtactg ctggcagaag cttctaagta agctaaaatt ttaggcaata ccatataata
     4321 aaacgggtgt tacctttatc caatacgcaa tcaggcagtg ccgtacagag ccgtaaggtg
     4381 actgcacggt gctgcctttt tggaagaaaa ggagaaatac aacatgagca aaaaacctgt
     4441 tgcattgatt atcatggacg gctttggtca taatcccagt gattacggca atgcaatcca
     4501 cgcagcaaag aagccaaatc tggacaaata tatgcagggc ccgaataccc tgatcggtgc
     4561 cagcgggctg gacgttggtc tgccagacgg tcaaatgggc aactcggaag ttggtcacac
     4621 caacattggt gccggcagaa tcgtttacca gatgctagta aagattacaa aggatattga
     4681 ggacggcaag ttcttcgaaa atcctgcaat ttccgcagca atggacactt gcaaagaaaa
     4741 aggaactgct ctgcacctga tgggtctgct ctctccgggc ggcgtgcaca gccacatttc
     4801 tcacctgtac ggactgctgg aaatggcaaa gcaaaagggt ctgtccaagg tatatgtaca
     4861 tgcatttctg gatggccgtg acgaaccgcc agcatctgcc gctggtttca tgaaggaagc
     4921 agttgccaag ctgaatgaaa tcggcgttgg taaaattgcc accatttctg gtcgttatta
     4981 tgcaatggat cgtgacaatg catgggatcg tgtagaaaag gcatatgatg caatggttct
     5041 gggcgagggt gtgcagacca ctgctgatcc ggtgaaggca atcgaggaca gctatgctaa
     5101 cgatgttacc gacgaattca tgctgccgac cgttctggaa aaggacggca ctgttaaggc
     5161 aggggacagc atggtattct tcaatttccg tccggaccgt gcaagacagc tgacacgttg
     5221 cttcgtagat ccggatttca acggcttcga gagaaagaac ggttattttc cggtacagtt
     5281 cgtttgcatg gctcagtatg atgcatccat gccgaacgtt tccgttgcat atccgccgga
     5341 agatctgcac atgacactgg gtgaatacct gagtaagtgc ggcaagacac agcttcgtat
     5401 tgcagaaaca cagaagtatg cacacgttac attcttcttc aacggcggca gagaacagac
     5461 ctttgacgga gaagaccgca ttctggtcaa gagccctgac gttccgactt tcgatctgaa
     5521 accggaaatg agtgcatacg aagtaacgga taaggttgtt gaagcaatca acagcgataa
     5581 gtatgatgtc atcattctga attatgcaaa ctgcgatatg gttggtcata ccggtgtctt
     5641 tgatgccgca gtcaaggctg tagaagcagt agacacttgt gttggtcgca tggttgatgc
     5701 aatcatgaaa aaggacggta tcgcattcat caccgctgat cacggcaatg ctgacaagat
     5761 gctggaagca gacggcacac cgttcactgc acacactacc aatccggtac cgtttatcgt
     5821 agttggcaag gattgcaagc ttcgcgaagg cggcgttttg gcagatattg ctccgacaat
     5881 gctagaggca atggaactgc cgcagccaga agaaatgact ggcaagagcc tgttcgcgta
     5941 aatcatgact ttagtaagta tcatataaga aaagcacttg tacagaatac tgtacaagtg
     6001 ctttttgcat ttcattatag atatatatca attagaaatc aatttccgtg tcgtagtagc
     6061 aagctttcag cagcttttcc atttcctctt gagagggctg acgcggattg gaaccagtgc
     6121 aggcatcggc aatagcattc tttgccactt ccggcagctt ttccaaaaat tccttttcat
     6181 caatgataga agtatctgcc tttacgccgc tcttaccgta attcttaatg ccctgcggaa
     6241 tatccaactg gtcattcatg gaacggagtt ctgcaatcaa cgaatcaact ttttcctctg
     6301 ttgttgcacc agacaaacca ataaaggatg cgatttctgc gtaccgattt gcagcttctt
     6361 catttttcga attatacttg atgacctttg gcagatacat tgcattggca caaccgtgaa
     6421 tgatgtgatc gccggtataa gccgcaccag tcttgtgtgc catagagtgt acgataccca
     6481 acagtgcatt tgagaatgcc ataccagcca agcactgtgc atcatgcatt gccgcacgag
     6541 cttccatatc tccgtcgtaa gacttcttca agttgttgtg aatcatcttg attgcatgca
     6601 gtgccaacgg atcagtataa ttgcagtgca gggtggatac atatgcctca atcgcatgtg
     6661 tcatagcgtc cataccagta tgtgcaacca gctttttcgg catagtttct gccagttccg
     6721 gatctacaat ggcaacgtcc ggcgtgatat taaaggaaac gctgaacaaa agcacagttg
     6781 aaaaagcaaa tcgataatta cgactaa
//
LOCUS       DAWBGR010000139         6802 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2890_length_6802_cov_3.032311, whole genome shotgun sequence.
ACCESSION   DAWBGR010000139 DAWBGR010000000
VERSION     DAWBGR010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6802)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2890_length_6802_cov_3.032311"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    985..994
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3210..3219
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1954 a         1416 c         1795 g         1617 t
ORIGIN      
        1 atgtgaaaat agtggcagga aatcactaca gtggcagtct caccagagaa cagtttttat
       61 tttacgaaat ccgaaccgtt tcgtcattga tactgcaagg ggaaagctgt gacgaaatat
      121 atcatgtaat atctactgaa aatttgtttc agtttccgac ggaaaagatg ataaaaagca
      181 tcacgaacac ttgctttaag cgcattgatg ccatgaatgc gccggagctt gtatatcatc
      241 tggcaaatgc ttcgctggat gttgcaaagc agatcaacct ctatgcaatc atgtgcgaaa
      301 atcgcattgt gtacgatttc atgacggagg tgatcggtga gaaatatcgt tcacaggaac
      361 ttgacttttc tgccaaggat gtaaatgtgt tttttatgga actttccgag aaagtttcca
      421 ctgtgaatgg gtggagcgaa tcgacacgaa aaaagctgaa acaagttctg gtgcggtttt
      481 tggcggagtg tggttatctg gaaacggtgc ggtcgcagaa cctgctgcca gtatatcttt
      541 atccggagtt ggacgaggca atacgtaaaa aaggggacgc agatgcactt gctgcattta
      601 attgttttat ttgagggtat agcgatgaat catattattt atcaattgaa cgaagtaaca
      661 aagagatttt cagatgaggt gtttttaagc aaccatggat tgtccaacga agtcggtatt
      721 catgtatttt gctacgatcc caaggaagaa atgcgggttt ccagtttttt ttcggatctg
      781 atccaaacgg gaaatcagtc gtttcatctg aaagaatgtg atctatatcg gattttcttg
      841 caaatatgtg aggaaaagcg tattctgaaa agcattccat ctatggagaa aaagaagggc
      901 tcagaatatc tcttgaagca actgcaaaag gcaatttcgc cggaagattt tgtaaaaaaa
      961 atgcagtatg caccccacga gcatnnnnnn nnnnctggac aatttgcagc atatcttttc
     1021 agacattcca gttgtgctgc tctatcccgg tacatacaat ggacaggagc tttcactttt
     1081 tgatgaattt caggacggga actattatcg tgcttttaat atgatctaag ggaggacttc
     1141 tcatgcaatt acgaaatatc tacaagtcag acatcaatcg tgacatcaac ggtgtcatta
     1201 aagtggcaca ggacgatgaa cgctctatgg tgcaggaatt gtccgaatat gtcatcacaa
     1261 aggaactgcg gcgtcatttc aatacttttt tgaatcacta tgagcattcg ctggagcagc
     1321 cgaccgacaa aatcggtgtg tggatctccg gcttttttgg aagcggtaaa tcacactttt
     1381 tgaagatgct ttcttatttg ctttccaatc gtaccgttgg cggaaaaaaa gcagtgcagt
     1441 attttgcaga taaatttgac gatccgatga tgtatgccca gctggaaaac tgcgtcaagg
     1501 ttccgacaga aacaattctt ttcaacattg attccaagag tcctttgaca aaagataaaa
     1561 cggcgatcct gcgtgttttt gcgaaggtat tttacgagca tcagggcttt tacggcaatg
     1621 atctgaaagt cgcaaaactg gagcagttca tttcaagatc cggaaagaca gaacaatttc
     1681 gggaatgctt tgcaaatatc aatggcggtt catgggagga ttctcgtgag tcctttgctt
     1741 tttttgagga tgacattgta gcagcaatgt ctgaagcact ggacatgagt gaaactgcgg
     1801 cacgcaactg gtttaacggg gaagaagaga ttgagctgag tattgagcag cttgtcaagg
     1861 aaattaaaga atatgtagag cgtaagggca aggacaaccg gctgctgttt atgatcgatg
     1921 aagttggtca gtacatcggt tcggattctg atctcatgct gaatctgcag acgattgtgg
     1981 aagagatcgg tacacagtgc ggcggcagag tgtgggtcat ggtgacctcg caggaggcaa
     2041 ttgacagcat taccaagatc agcggaaacg atttctcgaa aatacaagga cgtttcaaca
     2101 cccggctgag tctgagttct tcctcggtgg acgaagtcat caaaaagcgt attcttgcca
     2161 aaacagaaac cgcagaacaa ctgctccggc agcaatacga aaaaaatcaa caggtattga
     2221 aaaatctgtt tacctttagc cgtgcgatac tggatctgaa aggttattcc ggcgagggtg
     2281 aatttataga aacatatccc tttgtgccgt atcagttccg gctgatgcag aatgtgcttg
     2341 cagagatccg gaaacacgga aattccggaa agcatctttc cggcggtgag cgctctatgc
     2401 tttccggttt tcaggaggca gcacaggcga tccaggacaa ggatgaatat gcactggtgc
     2461 cgttctatct gttttatgac actgttcaca cctttttgga aagttcgatt cgccgtgtca
     2521 ttgatcgttg tcaggacgct gccgatcatc atgacggcat tgaaccatac gatgtcaata
     2581 ttctgaagct gctgtacctg gtacgctatg tggacgacat gaaagcgaat gtggaaaata
     2641 ttagtattct tatggctgag gacattcgca cggataagat ttccacacgc ctggaaattc
     2701 agcagtcgct ggacagactg gtatcgcaga actatgtttc cagagcaggc gatacctata
     2761 catttctgac agacgatgaa caggacattg cccgtgagat tcggaatacg cctgtagata
     2821 gtgctattat cacaaaagca atttcggaca ttgtcttcgg gaagctgttt gtcagcaaga
     2881 agttccgtta cggcaaatat gattttcatt acgaccagcg aattgatgaa actgtgatcg
     2941 gtcaattgac aggttctatt gcactgcact ttatcacagt ggcatcagaa atttatacaa
     3001 ccgatgattc tgtttttctg atgcggtcgg ctgctgacaa tgaagttatg attgttctgg
     3061 cagagagcca accctatttc aaagaactgg aagatgccat gaagatcaga cgctatgtca
     3121 agagtaaaaa tgtggcacag ctgccggaaa tgatccagag cattatccgg gcaagacaaa
     3181 accaggcatc cgcacatgaa aaacgtgcan nnnnnnnnnc tgctgccatc accagcggca
     3241 gagtgtatgt tgccggtgat aaactgacac tgaaaacttc ctcggtgaaa gactgcgtcg
     3301 agcgagcaat gggtattctt gtagaaagcg tttacacaaa gcttggatat atcaagaaga
     3361 attatgacag cgacgcagac attctgcaaa ttctaagcgg cagtcagcag atgaccttcg
     3421 caggaacgga gtcgccgaat gccgaagctg tcaaggaact gtatcagtat ctggaaatcc
     3481 agaaaatgaa acaattgccg atctccatgg gagatattca gaaacgttat caggcgatcc
     3541 cttatggctg gcgagagatc gacattgcgg cagttgttgc agaactgatc gcatcacaga
     3601 aactggtact gaaatacggc ggtgctgtcg taccgtctgg tgaaaaaaag atcacagagt
     3661 acctgcggaa aaaaacagaa atcgacaagg cgatcattgc attcaaggtt gcaccaccca
     3721 cggcactgat taaaaagtgc cgtgaatttc tgagcgatta tcataactgt acattgggtg
     3781 cgattccaga cgatgaggac ggtctggttc gctatatcac agacaagttt gacgcagagc
     3841 ggacagagct tcgggaacgg ctcgaaaagg aatacgcttt ctcggcatat cccggaaaaa
     3901 acgtggtgga aaacggtgca gccctctgtg acaagctgct tgcacagaaa aacgacagca
     3961 ttgcgctgct gaaaaaggca gtggaaatgc aggacgattt cctggacttt tccgaggata
     4021 tttccgacgt agacaccttc ttccgggtac agaagagcat ttttgacagt gccagagcac
     4081 aggcagaact ggcagacacc gaaaaagaat attttcaggc agaggacaac gcactttccg
     4141 ccatcgcgaa gatcagagag atcctcgcca tgccgaaacc atacaagcac atcagtgagc
     4201 tgccggaact ggtgcagcag gtgcaggata cctatgcaaa gttgctggca caaaagcgtg
     4261 aggaggttct cggagagatt cgtgcagcca tgggtgagat ccaccagacc gccgatacgc
     4321 tcaagcaggg gaacattgtc cagcgggcag atgcggcact gacggaaaag caaactgctg
     4381 ctgaacatgc agaaaagctg acaagtcttg atgcgatgaa aatccagatc agcaatatcc
     4441 ggcagcagta tctgcaaaag ctggtggttg tggcagagcc ggacgtggat accgtgacca
     4501 tgagccgcag caccatctgc cacaccatgc agctgaaaag cgaggcagac gttgacaaat
     4561 acgttgcaga gatcaggaaa acgctgctgc aaaaactgga cggacacgat gtgctgcata
     4621 ttatttgagg tggaatatga acaagaatgc gattaaaaat tttgccgtaa cggcaagagt
     4681 gacgctgatt caggcggtca cacagaaggc gtttgaatat gagatcacag cggacggcaa
     4741 aaatgatccg tcgcaggact cagtcaacgg tcagacgctg actgccgcag aacgcagcca
     4801 gcgtgcccag ctgatcgaac ggattcgggc gaacggcttt gcacggacca tggaagaggc
     4861 agcctacacc tggttcaacc gtttcattgc tctgcggttc atggaggtga acaactacct
     4921 gccgtcccac gtccgcattt tctcggacga gaacggcagc ttcaagccgg aggtgctgac
     4981 agatgcggtg aatctggaaa tcgacggact ggacaaggca ctggtgctgg aactgctgga
     5041 aaatcaggaa aacgaacggc tgtatcagta catcatcatc acccaatgca acgccctgca
     5101 cgagggcttg ccggagatgt tcgagcacat cggtgtctgg acggagctgt tgttcccgaa
     5161 gaatctgctc cgtgaggaca gcgtcattgc tcacatggtg gaggacatct ccgaagagga
     5221 ctggcaggat caggtgcaga tcatcggctg gctgtaccag tactacaata ccgagccgaa
     5281 ggacaaggtg ttcgccaacc tgaagaaaaa cgtcaagatc agtgcggcgg atattccggc
     5341 ggcgacacag cttttcacgc cggactggat tgtgcggtat atggtggaaa actcccttgg
     5401 cagactgtgg acagagggac acggcaagcc ggaacacgcc gactggaagt attatctgga
     5461 agaggcggag caggaggatg ctgtcagggc ggagctgaaa cagctgcggg aaagctatcg
     5521 ggagataccg ccggaacaga tccggatcat cgacccgtgc atgggcagcg gccatatcct
     5581 cgtctatgcg ttcgatgtgc tgatggacat ttacactgcc tgcggctggt cggagcgtga
     5641 tgcggcggtg tcgatcctgc ggaacaacct ctacgggctg gacatcgacc gccgtgcgta
     5701 tcagctggca tattttgcgg tgatgatgaa agcaagacag tataaccgcc gcattctccg
     5761 tggggagaac cagccaaacc ttgcgaattt cgccgacgtt atgggcgtga atacctctat
     5821 gctgagcggc agtctgcggc agttcgtgga gcagttccag ttcgccgaca cctatggttc
     5881 gctgatgacg gtgacagcac cggcggggct ggacgagatg gtggctgcat tccatcccac
     5941 gatcgggctg gacgagatgc agctgaaagc gatggtgaag ctctacaaga ttctgtcgca
     6001 gaaatatgac gtggtctgca ccaatccgcc gtatatgggc agctccggca tgaatgcggt
     6061 gctgtcggac tatgtgaaga agaactttcc ggacagcaag agcgatttgt ttgcgtgctt
     6121 tatggaaaag tgcggacagc ttgtcaagag gaacggcttg tatgccatga ttacacagca
     6181 cgcatggatg ttcctctcca gttatgaaaa gctgcgggag aaactgaaga tgaactccat
     6241 tgtgaacatg gcacacctgg gtgcaagggc atttgatgag atcggcggcg aagtggtaca
     6301 gacaacggcg tttgtcagct gcggcagacg agtgactgat ttcaagggga catatgtgcg
     6361 gctggtggac atcgtgggcg aatgggagaa ggaagccgag tatcgcagcg gaaaccaccg
     6421 ttacaccgcc aagcaggaaa acttctccaa aatccccggc tcgcctgtgg cttattgggt
     6481 gagtgagaat ttgttaaatt gttttgataa attttctaaa ataagcgatt acttttcgtc
     6541 gtgttctgga acaagtactg gtaataatga aaaatatctt agattatggc atgaagtaga
     6601 taatataaaa atagaattta atcaagaaaa taatgaagtc actaaaaata aatggtatcc
     6661 atgtaataaa ggtggggctt atcgaaagtg ggctggtggt tcagaatatg taatagattg
     6721 ggaaaataat ggaaatagga taaaaaatga acctaaagca tttttaagaa atgtttcaaa
     6781 aatatttcta cctggtatta cg
//
LOCUS       DAWBGR010000140         6759 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2911_length_6759_cov_4.249851, whole genome shotgun sequence.
ACCESSION   DAWBGR010000140 DAWBGR010000000
VERSION     DAWBGR010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2911_length_6759_cov_4.249851"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1470 a         1837 c         1928 g         1524 t
ORIGIN      
        1 tcagtcagtg ctacgcactg ccagctcagc acaaggcacg ccctacgggt gccctaaagg
       61 ggagcctaaa tgtaggagaa atagccttcc ctttagggga gggggaccgc cgtcaggcgg
      121 tggaggggta accccgtgaa atctcttgct tgatgtaaag atttacgaac taacccccca
      181 gtcacctgcg gtgccagctc ccctaaaggg gagcctatta caaataaacc tcccctgaaa
      241 ggggagggga accagcgaag ctggtggagg ggttcccatg tgaagccttt ttcctgatgc
      301 attagatttc atagaaaaaa cctagaattc tacggataca gcacaacgat tcagacaact
      361 tcatatctcc ataaacgcac agaccggctc tccagtctgt gcgttctgat tgttgagcca
      421 aacattttca aattattcct tgacacacat tctgcaatat ggtacactaa atttaaccga
      481 aaaggcaatt cagcgaaagg aatgaacaac acgatgtttc gaagttatga cctcagcggc
      541 gtgtgggact tttccatggc acagatggca aagggcactt tccccacggc gttttccgat
      601 accatttcac tccccaatac tacctctctc gccaaaaaag gcaccccaaa tcccagacgg
      661 gaaaccggtt ttctcaccga tgcctacgct tttgaagggc aggcgtggtt ccgcaaaaaa
      721 atctatatcg atccggaact gatcgaccct gacaccggct gctgccccat gaagctgaca
      781 ttggaacgca ctcgcatgac aaccctctgg atcgacggca ggcgggtggg cagctgtgac
      841 agtttgtgca cgcctcacgt ctatgacatc accgcctatg tgacaaagcc gctggtggag
      901 attctggtgc tggtggagaa caccggctat cccaccaagg gcggccacct cacctcgccg
      961 gacacccagt ccaactggaa cggcatcacc ggtcagatga cgctggaagt gtttccggat
     1021 gtctatgcag accatgtgca ggcgtatccg aatccggcgg aaaaaaatgt caccctcacc
     1081 atgcagcttc acggggcatc gacggcaagc tttaccgcca ccggcagcgt catggaggat
     1141 gacgaggagg acagcattcc gctgtttcag gcacagctga cggcggacga aaacggcatg
     1201 gcttcggtga cgatcccgct ggacgaggcg tactatgcgt ggagcgaata tcaccccgct
     1261 atgtattatc tggtctacaa catcgacgag gcggactgcg gctatgtcag cttcggcatg
     1321 cgggagttct gtgccgacgg cagccagttc acgatcaacg gtcagcccac tatgctccgc
     1381 ggcaaacacg acggactgat cttcccgaaa accggctacg ctcccacaga tgtgaacgag
     1441 tggctgcggg tgctgggcat cgccaaaagc tacggcatca accactaccg cttccacacc
     1501 tgctgcccgc cggacgctgc ctttactgcg gcagatctgc tgggcatcta catggagccg
     1561 gaactgccgt tctggggtac cattgcggct cccggtgagg agggctacaa cgaagccgaa
     1621 cagaactacc tcattgcact gggggacaag atgctggaaa ccttcggcaa tcacccctcg
     1681 tttgtgatgt tctcgctggg gaacgaactg tggggcagtc cggaacggct gggggagatc
     1741 ctgcggcact acaaggaaaa ggacagccgg cacctgtata cgcagggctg caacaacttc
     1801 cagcacttcc cgctgatggt gccggaggac gactattttg tcggcgtgcg gctgagcaag
     1861 gaacggctgc tccgcggctc tttcggcatg tgtgatgctc cgctgggaca cgttcagacc
     1921 gaccgcccca gcaccatgca ccagtatgac gacgtgatct ttccccgaca gactgccgga
     1981 gaggacagcg gcgacacgga ggagatcgag atccagtacg gtaccggcgt gaagaaagtg
     2041 caggtcagca agactgccgg cggactgatc cccaccaagc cggtcatcac ccatgagatc
     2101 ggacagtacg aggtctatcc ggattttcgg gagatcgaga agtacacggg gcctttgaaa
     2161 gcacggaatt tcgaagcctt tcgggaacgc cttgctgcca ggggcatgct gcatcaggcg
     2221 aaccggttct tccggtgcag cggggcactg gcggctgcct gctacaagga ggagatcgaa
     2281 gccgccatgc gttcccgcta tattgccggc ttccagctgc tggacttgca ggacttttcc
     2341 ggacagggca cggctctggt gggcatgctg gacgcatttc tggacaacaa ggggctgatc
     2401 tcgccggagg aatggcgaat gttctgctcc gactgcgtac tgctggcaca gttcccgtcc
     2461 tatgtgctga ttaccggcga gatgtttgcg gcaaaggttt ctctgcggac agtgctgccc
     2521 cagtccggca cggcggaatg gatgctgatt cgggaaaacg gcgacaagct gggcgagggg
     2581 agattttcgg tacaggcgga atccggtctg acggagatcg gcagcatctt ctgccgcatg
     2641 ccggaggagt tgcagcggcc ggaacgagtg cacctcattc tgtcgctggc ggacaccgat
     2701 gtctgcaacg tctatgacct gatgctctat ccggcgatcg ccatgccggc actggaagat
     2761 cagggggaac tgtgtgtcac ggagcagctg gaaacggcac tggcagctct ggcggcaggg
     2821 aaaaagacgg tctttttccc cagggaaacg gcggagagca tacagggctt ttactgtacg
     2881 gatttctggt gctatcccat gttccgcgac atctgcaact ggatgaaaaa gccggttgct
     2941 gtggggacaa tggggctttg cattcaggac gaccaccccg cactggaact gttccccacg
     3001 caggagtaca gcacgcccca gtggtatgac attgtgactg cggcggactg caccatcctg
     3061 gacgacacgc cggcggagtt cacgcccatt gtacagatga tcgacaactt cgaacgcaac
     3121 cacaagctgg gcattctctg ggaagccaag gtgggcagcg gcagtctgct ggtctgcacc
     3181 agccgcctgt ccgagatcgc cacccgtccg gaagtgcgtt ggctggcaaa gagcctgctg
     3241 cactacgcgg catcagaggc attcgccccg cagcagagcg tgacggcgga acagctgcgg
     3301 aagtggtttg gggtatgagg gattttactg tgaagcctga agtggaatgg aaaagatttc
     3361 acggggttac ccctccacca gcttcgctgg tccccctccc ctttcagggg aggctctttt
     3421 acacatttag gctccctttt gtgtataggc tcccctttag ggcacccgca gggcgtgcct
     3481 tgtgctgagc tggcaccgca ggtgactgag gggtgcttcc gtaagctctt ttacgtcaag
     3541 caaaagattc cacaagaacc cctccaccgc ctgcggcggt tcccctccct tttcaaggga
     3601 ggctattctt ccagcgggga cacggtgcaa tctgtcccta cgattccaca ttgattccac
     3661 gatctcaaag gagtatacac atggaaaaat tattactcat tgcaattccc gacacggacg
     3721 gcgtggagca ggtcgcacgt cggctgcggc tgcgaacggt gctggtacag ccggaacagt
     3781 atgcccgcag tctggggcag cttgccggtt atcctgctgc cgcggcagag gaaacaccgg
     3841 cgttgcccct gccggaaacg gggctgctgg tgatgtgtgg gctgaaaaat tcccacatgg
     3901 acaagctgct ggctgccctc cggaacgccg gcatcggcat tccccacaag gcagtactga
     3961 cccctaccaa ccagaactgg aacgtctacc ggctggcaca ggaactggaa cgggaacggc
     4021 aggcgatggg ggagaagtga acggctgttt gaaaattttc tgggcaagta gggcgtgtag
     4081 aagatgttgc ggaagaaccc ttccaccgcc taacggcggt tcccctgaaa gggcacccgt
     4141 agggcgtgcc ttgtgctgag ctggcaccgc aggtgactga ggggtgcttc tgtgaaatct
     4201 aaaacggaat ggaaaagatt tcacggggtt acccctccac cgcctaacgg cggttcccct
     4261 cccctaaagg ggaggctatt tctcctacat ttaggctccc ctttagggga gctggcaagc
     4321 cgtaggcttg actgaggggt tagttcgtaa atctttacat taagcaagag gttttacgag
     4381 agaacccctc caccgcctaa cggcggtccc ctcccctttc aggggaggct cattttagag
     4441 gtgactgagg ggtttccctc tatctccacc aacgcaaaaa cacggcacac ccaaagcggg
     4501 cggtgccgtg tttttgcata taatggaagt taaaaaatga aattgggaaa tgtcgtaatt
     4561 ttacgggctt tgtgcggtat tgccttaacc cagcaggaac ttcatcaatg tgacagcgtc
     4621 ctgaatgttg aaactgccgt cagcgttcat gtcagcacgc tggaaattcg gatcagtttc
     4681 cgacggtaca agtgcaactt ctttgaacag catatcccgc atgatcgcca gatcgaagcc
     4741 gttcagcatg ccgtcctcgt tgacatcgcc gcgaatgaca ttgccgaccg gttcggtgac
     4801 agtggtagtg gtagtggtgg tagtttccgc agtggtttct gtggtgcttg tagtggtgac
     4861 taccggatcc ctgccctcta cgatggcgta gaacgacggc tttgcggtgt agttctcatt
     4921 gaacagcagc ggggtttttg cggctctcca gctcttgtcg tcggtggtgc cccagaatac
     4981 cactgcggaa atgctgtctg catactctac gcatgcgtcc atgatgccct tgtacatctc
     5041 tgcctgcttc tcgaagccgg cttccgaggt gtcagaggtg gtgatgtcca actcggtgac
     5101 ctgaatgtcc agaccggttt cgacaaactt ttccagagcc ttcttatagg tggaaacgtt
     5161 ggggaatcca acgtccagat gggactgcat gccgataccg tcgatcagtc ccttgtcttt
     5221 cagttccaga gccattttgc agatcgcatc ggttttctgc gatatgtact cgttgaagtc
     5281 gttgtagtac agcttggtgt ttgccggagc gtactttctg gcatactcaa atgccggttc
     5341 gataaaggag ttgtcgccga ataccttgac ccagccggaa tagttggcat tctcggactg
     5401 tccgccgccg gtgcggggag agccgttgtc cagccatgct tcgttgacta cgtcccatgc
     5461 gtagaagtct accgtcgggt attcttcttc cagagccgcg aaaacgttct tgatgtagtt
     5521 ttccatacgc tggagcatca cgtccttgga tacccagtcg ccgtcgtcct gaaagccttc
     5581 cttaaagaac cagtccggtg tctggctgtg ccataccagc acgtgtccgc gaaccggaat
     5641 gttgttgtca cggcagtaat tcagaatggt tctggcactt gccagattga tgaccggatc
     5701 agtgttgctg ccggaagcga gggtagcggc tttgtccaga atggattccg gcttcagctc
     5761 gttgcccagc gtgatgctgt tgaaatgctt ggcgatcaga tccttggtgg actggggagc
     5821 cagttcctca gtggtcactg cggtgccgat cttgaaatcg ttggcatata cgtccttgag
     5881 ggacggaatg tcgttctgaa tggggtatac cggagccgtg cgtacttcga aatcgtcgat
     5941 gtacatggtg gctttgtcgt tgcactcaaa gtacaggtat accttggaaa cgtcctcgga
     6001 gaagctgaac ttcacgttgg agaatgttgc ccagtcgccg ttgctggtct gttccttcaa
     6061 attgctgtag tggcgttcgc cgctggcatc ggtgtattcc atgctcagct tgatcgagtt
     6121 gtaccactca gtctttgctg cggcactgac ggtgtactgc acgccggctt cgcacttgtc
     6181 gtccagcagg aactgcgggc cgttccagtt tttcgctctg tcgctgacgc acatggagta
     6241 agagccgctg tatgcctgcg aatcggttgc ggtaatggtt tctacgccgc ctctgccggt
     6301 gaatgccgaa acatcgccgt cctcgaagcc actgctgtag atctctgtgg tgccgtcgtc
     6361 ctctgccgca ctgacaatgc cggcagaact gccgctgaac gctgtcgtta ccggaactgc
     6421 tgcacagagc atcacggcac tcagcacacc ggcagttaca cgagttctga tttccttttt
     6481 catgaatgtg ttcctccctg ccacaaaaat attctatttc attgtagcat gcggagcaga
     6541 aaaagtcaac taaccgcacg gatatttgta tgaaatcaca aaaaagcacg cctcattttg
     6601 tgcaaaagac gtgctttccg gttggggaag tggcggctgc cacttatata agtattgtaa
     6661 tttgttgttt gacatttttt gcaaagtgtg gtatactgga ttttgagggc atacctgaaa
     6721 cggtaggcgg ctcgctatcc ttgggcgagt ctgccgtcg
//
LOCUS       DAWBGR010000141         6709 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2944_length_6709_cov_3.371506, whole genome shotgun sequence.
ACCESSION   DAWBGR010000141 DAWBGR010000000
VERSION     DAWBGR010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2944_length_6709_cov_3.371506"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1656 a         1721 c         1613 g         1719 t
ORIGIN      
        1 cgtggacaaa cccaatggaa aaatgtatga agaaaagttc aagcagatcc agcaggcata
       61 ccagacgatc atggacgaac gccagcacaa gaactccggc ggatatggcg acttctacaa
      121 ccggaacacc tctgcgggag ccggcgacga gaacagcgtt cacttacagg ctgccgtaaa
      181 cttcattcgc aacggcagat ttaccgaagc tgtccgcgta ctggaaggca tcagcaaccg
      241 aaatgcccag tggtactatg tttctgctgt agcacattct ggtgccggca acaacgctat
      301 ggctctccag tatgccagaa ccgctgcaca aatggaaccc aacaattatg aatatcagaa
      361 actggtacag cagctggaat ctcctggttt ctcctaccag caaatgcggc agccttatat
      421 gggcggcggc ttcgaagaca gcggctgtgt tcgctgctgt gcggcaaata ttttgctgaa
      481 cctgattctg gattgctgct gcaattgtac ctgacacaaa gaaaatggtc ttatattttg
      541 gaagtattgc acaatatttt ttcaaaaaac tgtaccatgt gagaaaaatc atggcttctc
      601 tcggaatttt cacggaaaag tgtttacttt tcccgcggaa tatggtatga tgacaattga
      661 ggtcatacca atttcagaag cggctccccc acagccgctt tgaagaatga ggagattcta
      721 tgatgttttt caatcataaa aatacagagc cggagcaatc gcaaagttct aatccatttt
      781 tttactatgg cggaaattat cccggagaaa catcagaacc cttttatcat tccggaatca
      841 agaactacaa cctgcgtgtt tctgttgcaa cacacatcgg aaaagtgcgg ggcaatcatg
      901 aggacaactt ctatctggaa gggttgtata tgaacgacat attccggaac gatttttctg
      961 cttcctatac cattcattac gcagacggca tgagttttgc ggtgttcgat ggtatgggcg
     1021 gagcagcgta tggcgaagtg gcatcggaaa ttgcggtgca gacactccga aaatacgaga
     1081 aaaagctgaa acacgcagac gggacacgta tgctggatca gctggtttct tcttatacca
     1141 cggaggcaaa caacgccatt tgcgacatgc tcacagaaaa gcactgccaa accggcggaa
     1201 ctactttttc catgctttat tttctgggag atgcggtaaa gttatattat ctgggggaca
     1261 gccggatcta tcgctatcag acggatcagc tgacgctgct cacccgcgac cataccgttg
     1321 ccaaccaaaa agtagaagct gcgatctata cagaggaaga agccaagcac agtcccgatc
     1381 agcatcggct gacattgttc atcggctccg accgcaaaaa gacggggtta aatgcggaca
     1441 gcagaccccc cttgccgttg gaagtcggca caaagtttgt gctgtgcacc gatggactga
     1501 ccaatatgtg ttcggacgaa gaaattgcag agcttctctc cgaaaattat ttcaatgaag
     1561 ccgcagtgct ggtgcaggct gcactacaga acggtggcgt ggataatatt acctgtatcg
     1621 tgctggaagt gcttccggca acagacgaca aggaaactga ctgataaact gaggtgacag
     1681 catgtaaaac tgcatgttgc ctttatatcc ggtgataatt gccataacaa gcaaaaccgg
     1741 cacaagatgt tgtgaaagca tcttgtgccg gttttttgta tcattttatg atttttgaaa
     1801 tcagatacgt tctaccttca tcatattggt ggtattagaa acgcctaccg gtgcaccggc
     1861 agtgattact acaatatccc ccttgttgac caaatcggaa cggagggcag cgtgaatcga
     1921 tgcatcaaac agcgagtcgg tattggaaac ttcatcgacc agcatcggat aaacgcccca
     1981 gctcaagttc atctgccggc gtacccgttc gcttaccgta cagctgatga tcgggcagca
     2041 cggacgatac ttggaaattt ctctggcagt gacaccggac atggttactg tcagaatggc
     2101 agctgcgtcc agatcgtgtg ctgtggtaac tgtcgcgtgg gcaatcgcat tggcaatgtc
     2161 cgcgttcttt ttggtacggc ttgcagaatg catgccctta tagtcaatat cgtcttcggt
     2221 ggtacgtgca atcagatcca tggtgcggac aacctctacc ggaaaggcac cggcagcggt
     2281 ttcgccggaa agcatgatcg cactggtacc gtcatagatc gcgttggcaa cgtctgtgat
     2341 ctcggcacgg gtcggacgcg gattggtaat catagattcc agcatctgag ttgcagtgat
     2401 gacctgcttg cccgcctgat agcccttgcg aatcagctgc ttctggacag acggaatctg
     2461 ctcaaagggg atttctacgc ccagatcgcc gcgggcaacc atgatgccgt cagaggcttc
     2521 cagaatctcg tcgatattcc gaacgccttc cacattttcg attttggcaa tgatgcggac
     2581 atcgaaccag cccagactct gggtatactt ccgcagatag tcaatatccg cagaagtccg
     2641 cacgaagctg gcagcgataa agtcaaatcc cattttcgct gcgaattcca agtcgttttt
     2701 atcgctttca ctgaggtatg gcatagacag ctgtaccccc ggcacgttga ttcccttatt
     2761 gttcgaaaga acaccgccgt gaatgacatg gcagacaatt gtagtgacgg tcaccttttc
     2821 cacaaccatt tcaattacgc cgtcattcac cagaatgtgt acgcccggct tgacatcacg
     2881 gggcaggttc ttgaaagaaa tgctgcctac cttatcatca cagagaacat catcagtagt
     2941 cagtgtgtag gtgtcgccgg tttcaatggt gactggctta tcatctacaa atttgcccag
     3001 ccgaatctcc ggtcctctgg tgtccatcag tgtggcaata ggcagaccca gttcttcccg
     3061 caggcgggag atctgctcaa accgtttttc gtgtgtggcg tggtcgccgt gggaaaagtt
     3121 aaaacgagcg acgttcatgc ccgaaagcat aacttgccgc ataacggaat cgtcatctgt
     3181 cgccggtccg atcgtacaaa caatctttgt ctttctcata agtaattcac gcatcctttt
     3241 ggttgtatag attctttcat atgcttatct tttattagta taccacaaaa cacagaaaat
     3301 gaaaagttaa ttttacgaaa agtattttac ttttttcttt tctgagataa gcattctctg
     3361 aaatttcttt gattatcaaa tcagctgtag gattcgtcca aaaatacctc aagattttcg
     3421 gaaagaattg cacgattttc cgaatccgaa agttccaatg caaaaaagcg ttccagttcc
     3481 tttctataat cccacatggg gtagtctacg ccaaagaagc agttctctgc cccgaagtgg
     3541 gacagcagct ttcgaatctc ctccggcggc aaaaacggca ggctgctgct ggtatcgaac
     3601 ctcagccgtt catccggctg caaaatggcg gttgcctctt tccactgtcc ccagccgccg
     3661 aaatgtgctg caatgatccg caatttcggg aactgccgca aaatcattgc cagacggtac
     3721 gggtgggaaa agtcggactt ggcatcaccc atatgcatca ggatcggcaa accggagctc
     3781 tggatcatct cgtacatggg atatgcccgc ggatcatcaa tgggaaaatt ctggaaatcc
     3841 ggatgcagct tgacaccgcg aagctgtagg gatcgcagct gctgaaaatc tgcctccacg
     3901 ttttccgcat aagggtgcag cgttccgaaa gcggtgcaca tggggtatgc cgccgcacaa
     3961 gatgccagaa aactgttgat cgagtgtacc tgtgtcacgg tagttgccgg agagcagacc
     4021 agaaaatgat cgataccagc agccgtgcca ttctctaaca tttctgccac tctgccatgg
     4081 tactgcatcg gcagatcata aaaatgagaa atcccctctg ttgccttttc cgcgatcttg
     4141 tccggaaaca catgggcatg agcatctgtg atacgataac cgtttttcaa gaatcattcc
     4201 tccagctttt tgatttttgg gtggcttgcc attaaccgtt tggtgccgac ctgatcgaat
     4261 ccgacttcca gcattgcatc atttgccatt ttggtaattg aaagcacggt gccgtcgccg
     4321 aaaaccgcat gtcttacccg ttcacctacg tgaaattcct tgggttgtgc ctgatacacc
     4381 cgtttcttgg acgcagccat ttgctgctgc aacaaagaaa tagattgcac agactccgca
     4441 ttgcctgccg gaatcccccg cttggactgc cgccgggaag ttttttcttc cagcagtgac
     4501 ttgtcgattt cttccagaaa ctgcgagggt ggatttcgca tggtcatacc aaacagcata
     4561 cggctggcag cagtagtcag cgtcagcttc tccttggcac gggtcagtgc tacataagcc
     4621 agacggcgtt cctcctccag atcctgcggt gtgctcaggc tgcgatttcc ggggaaaaca
     4681 ccctgctcca tacccaccag aaaaacattc cggaattcca gacccttggc ggaatgcact
     4741 gtcatcagca tcaccgtatc ctgatccggt tcgtatttgt ccacgtcagt gtacagagaa
     4801 atttcttcca gaaatccgcc gagagaagct tcttcagctt cgttctggta ggccaggatc
     4861 gaggttttca attcctcgat gttttccaga cggaacgtgc cctcctcacc catgctttcc
     4921 agcatgccat ggtagccggt tttctccaga atcgtatcca gaaactgttc cagcggttgt
     4981 tcccgttctg cggtgatcaa cgcctcccag agttctgcaa actttttcag tgccgttttt
     5041 ttcttggaaa gtgccggata ctgttccaca ttctgcagga cctccagcgg agaaaggtgc
     5101 agatcctgcg aaatttgcag cagcagggca agagtagcgt tgccgatgcc ccgcttaggc
     5161 tcgttgacga tacgttcaaa ccgcacacgg tcgttgggat tttccacgat gctcatatag
     5221 gcgagaatat ctttaatttc cttgcggtca tagaatcgtg tgccgccgta aatgcgatac
     5281 gggattccct tatgtaccag tgcccgttcc agcatgttgg actgggcgtt catacggtac
     5341 agaatcgcat ggtcgctgta cttttcgcca gctttcacct gtttcagaat agtatccgaa
     5401 acataggcag actcgtccga ttcgtctgct gctctgtacc acgtgatctt ctcccctgct
     5461 ccggcattgg tccagagatg cttggacttt ctgccggtat tgttggcaat cacgctgttg
     5521 gcagcgtcca gaatattctg ggtggaacga taattctgtt ccagacgaat cacggtgcag
     5581 tccggaaatt cttcttcgaa tccgagaatg ttttcgatcg tcgctccgcg gaaccggtag
     5641 atactctggt catcatcacc cacaacacaa aggtttccgc tggcatgagt cagcagcgaa
     5701 atcagctgat actgagcatg gttcgtgtcc tgatactcgt ccaccagtac atagggaaaa
     5761 cggtactgat actttgccag ttcctccgga aaccgccgga acagttccac agtcaggtaa
     5821 ataatatcgt caaaatccag agcgttggat tcccgcaaat gccgctggta cgccgcatac
     5881 agtctggcaa ccgtctgctt gcggtagtct tctccggcat cttcccacat ttcctccggc
     5941 gagatcatcg catctttggc gttgctgatc tcctgtacga taccccgcgg tggaaactgc
     6001 ttttcggaaa cattgcagtc tgcaaggcag cttttcatca gctttcggga atcgtccgca
     6061 tcataaatgg caaagtcact gccgtatccc agccgttcga tgcttcgccg caggatctgt
     6121 acacaggcag agtggaacgt ggaggcatgc acggaagatg ccagttcctc tcccagggtg
     6181 cttgccagac gtgcccgcat ttctccggca gccttattgg taaaggtgat cgccagaatg
     6241 ttccacggac ggatcggggc aattgccagt atctcccgca ggcgttccac gtcttccggt
     6301 tcttttccgt caatgtaggc ttgcagccag acggcatctt cttccggcag gtagccgtct
     6361 gcctgtgcgt agccgtcacc aaaataaatc atgttgacaa ttcggctgat aatggcggtg
     6421 gtcttgccgc tgccggcacc ggcgagcacc agaaccggtc cgtttacgga aaaaaccgcc
     6481 tgctgctgtt gttcattcag agaatgaaaa tattttcgga atgcctgctg ttttaatgca
     6541 atgcactctg cttctgtcat agctttctct ccttactttc gggatttcag attcgcatat
     6601 gtgtttaggc aataccgcac aaagagcgtc tggcgacttt gcacgccatc tgcgtgcaaa
     6661 ttttccgtca tatttcacaa aaatgctcgt gaatacatca agtattcac
//
LOCUS       DAWBGR010000142         6703 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2946_length_6703_cov_5.626053, whole genome shotgun sequence.
ACCESSION   DAWBGR010000142 DAWBGR010000000
VERSION     DAWBGR010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2946_length_6703_cov_5.626053"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1478 a         1775 c         1735 g         1715 t
ORIGIN      
        1 agggcagagg ggaccgctca gtacgagggg gaccagcgaa gctggtggag ggattggaag
       61 taaaatcttt tgttcaagtt taggtggtac tcaaattaac aacaattcac tacaaacaaa
      121 aagcccgccg cattccatat gccggaatgc ggcgggctgt cgttttatcc agttttttcg
      181 ctggtcacat tatgcgaccg gtaaagaagt gaagcttctc agcaggtact gcatcaggac
      241 ggtcacgtct gtgatgtcca gctggctgtc cttctggcag tcggagttgg caagctgtgt
      301 atagttcagc tgcacactgc cgttcaggta cttttgcagc agcacagcat ccagaatgtc
      361 gatcgttccg tcacagttga catcaccgta cagcgaaaca tatgcttcgt cgacacggtt
      421 catatcgatc ggcgtggttt ctgccggttc tgtgtcctgc tgtgatgcag tggtcgttgt
      481 ggtttcaacg gtcgttgtag tggtttctgc cggtgcggta gtttcctctg tggtcgtggt
      541 ctgggcagct gcctgcttca gatagtccat agagcagtag ccgctaatgc cgttatagga
      601 caccgctgcc cacttgccgt tgccggaagt gacagtcacc tgtgccccgt tgggaatgct
      661 gcccaccttg gaagcggacg ttgtgggggc actgcgaatg ttcagagaag atgcgttcac
      721 actgtaggtg ccggcatagc tttcgctgaa atccgaagtg gctgcggttg ccgccgtggt
      781 ggtggtctgt gccggagccg tggtttctgc cggtacggtc gtctgtgcgg cattctgctt
      841 caggtaatcc atagagcagt agccgctgat gccgttatag gacaccgccg cccagctgcc
      901 gttggcagaa gtgacggtta cctgtgcccc gttggggagt acgcccacct ttgcaccaga
      961 ggtagacggt gtgctgcgga cgttcaggga cgaagccgtc accgtatatg taccggcata
     1021 gctttccgag aaatcggaag tgtccgcaga accaccgccg ctgccgttgt ccccgccgcc
     1081 ggaaaccgag gcatcgcccc agcccagcga aacaatggtg ttataggcat tgttcagacg
     1141 gtcggtgtaa tagctggaac tgttcgccag tgcagtggtg tgcagctttg acagtgtcac
     1201 gctgctgtaa gagccgccgc ctgccgccgc gttcaggatc cgttttgcaa cgcccatgcc
     1261 gcggttgtac agttcggcat aatacaccag cacgccggcg ttggagatgc ccagattctg
     1321 tccggcactg atatagccct gtacgtcact gtatgccagt gcgtcctgtt tggactttcc
     1381 ggcggaggta gtcagcaggt tggaagctgc tgtgctctct gcactggaaa atgtacggga
     1441 gttccagttg gaagcattca tcacatcgtt atagaacgag cttcccagaa tggattgtgc
     1501 cgagccggtg tctgcgtttg cgatcgaacg catcagctgg agggcacggt ctgcatgcca
     1561 ctgtaacata ccgatgctga ctgcaccgtt atcgttggga ttgatcgtac cataggtacc
     1621 ctcgctccga gagatgatct gtacagcggc ttttgtcatt tcgtccacgg aaacggcaga
     1681 cgcggaaaac gtcaccgcaa aggacgaagc gaccaaagcg aaaatagtaa gacaggataa
     1741 aacgactgcc atgattttct tacgcataaa aacgctcctt tgagaatttt gcgttgtcag
     1801 cagtcgctgc atcgcagatg tacggagaaa cctgccgctg caatgcgttg tgctgcacac
     1861 gcctgtccgg tcctgaaagc ggaagggata cgccgtgctc tgccggcatt gtcctgtcgg
     1921 atcaaaggat ctgtcacagc cagacgatcc gccccagaac ttgtgtgccg cctgcattgc
     1981 ctcgcccgcc tgtctgtcac aggcaagggc gaacggcaga catcatttcc gtgaggagag
     2041 gttgtcccct cacggcggtg gtcaccgtac aaatgctatt ataccatgtt tgtaaccgtt
     2101 ttgcaagact ttccggcagg ttctccccga acttttgcat atctgcacaa ttttttgtgt
     2161 ggaaaagtgt tgcttttcta cgaatttcag aggaaactga gcaagattct cctatccaaa
     2221 ttgtaacttt ttcagagatc ctgtcatatt ttcgcctgac aaaacaaaaa aagtccggcg
     2281 gctgccggac tgggaaaact atttttctag ggaatttgtc ttattgcaga tgaatgctgt
     2341 tgatcagcgt gtctgcaaat tctgccgcac tgtcctcacg gtaacagtaa cgaaccagca
     2401 gccacttgga ctgatcgccg gttttgttga tgagccacgt cacgctggtg gtgggatctg
     2461 caccggctgt gtggttgatg accagctgat atgccgcata gccccacacg gtgcactcct
     2521 tggaggtgac cagttcattg ccgccggcac ctgtccacat ggatttgtac tgttccattg
     2581 ccgcatagaa ttccgtatcg gaaaccgtca tggacttggc tgccacaatg gcatagtccc
     2641 gatcgcggtt catgaacagg tcatagccgt catactggga agccagcgat tccagcccgt
     2701 aaaaatggct ggtgtcagtg acccgttccc acttctcagg caaatcgatc tggtagccaa
     2761 tggtgtcgtt ggaataggac agcggctccg gttccggtgc atcagccaac tggtcgttct
     2821 ctaccacata gccggtttca ttcagataga tgtggtctgc cagctgatcg cggtagttct
     2881 ggaactcgcc ggtcttgtcc cgcatggaaa agacgtacat tttgtcctgt gcaggacagt
     2941 tgaccgccag aatcgtcaca taggtatcct ctccggcaat tgtccccagt gtttcgccca
     3001 caaacgcctt acagggcata ttttccgcat gggcaatttc cgtttcccga aaatattgca
     3061 gctgcggcag cgtggtcgaa aagttaatca gttcgaacag gtcgtcttcc tgaaattggt
     3121 tgaattcttc ttccggctgg gcatcatacg ctgcgacaac aatggcatca tcgctttccg
     3181 tgccgtcaaa ggacagtctg ccggaaacag cagagttgcc ggagccggtt gtaaaggtgt
     3241 ccggcacttc taccgcacac ccgattccgt ccatggcata cggtttcagc tggaaatccg
     3301 cctgctccac cgtcggacgg ctctccgcat cagctgcttc tgtgctttct gatgcggcac
     3361 tggtttccgc cttttgggca gcgtttttag caggatacga ctttccgcag ccggtcacag
     3421 ccagcagtaa tgccgccgct gctgcaattc ccccaaggca agtttttctc tgtatcatag
     3481 aaatgcattc ctttctcatg tcatctgttc atcagattct ttctttttat cacaaatgcg
     3541 acaaaaatag aataccatat tttctcgaaa aagtcaagtt ccaaagaaca gaatgttgca
     3601 ttttgtcaag gcggcactga ctggcaacaa aaagccgcac catcaccaga gaactccggc
     3661 aatgatgcgg cggcttgcat ttgacatttt ttgcaagata tgatattgaa aggcatatca
     3721 ataatggtag gcggctcgct atccttgagc gagtctgccg tcgccatttc cgctgggcga
     3781 cgggctggtt tgtttctgct cccactgctc atttgaacag atgggaggtg atcagtatga
     3841 tggatcaata tgtcaccttt ggtgatttgc tgaagatttg cagtgttctg ctgtccttag
     3901 ccaatttgat cgtatacatt tactataatc ataaaaatag taatcagaat caccacggca
     3961 agaaaaagaa ataaccgccc cacttctctc aaaactgtgg cggttattct ttaacctcta
     4021 tgcagtggga gcagaccgtc tactggtatg ctctctcttt atttttatta tacgccagaa
     4081 tgccgcattt gtcaagggtt tttttgccgg aatacgcggc aatttttatg ccgcatcagg
     4141 caaaacagtc acaatatgca ccagaaattt catcagcagg gtggcatcat cggcattgac
     4201 ctcgccgctg gcatcacagt cggcattctg atactgctgc ttgttcagca ttaccacacc
     4261 agaaaccgcc ttgttggtca gcacggcatc ggatatatcc accttgccgt ccagattgac
     4321 atcaccatag agcacgctgc cggtatccgg tgcggtggtc gtaggtgccg gagcaattgt
     4381 aattgtgatc gtactgccct gcaccaacgg tgtgcctttt tgtccgttca cattatccag
     4441 ccacatggca tcggcaccac tggcggcacg tgcagacaag ttgaccacat agacatctcc
     4501 tgcctctgca tcgtccggca gccggaaccg aagcgttccg atctttcctg ccggaatatt
     4561 gctgaccgat gcatttacca gccagacaaa accgtccgcc tccgcactgg agtacgcacc
     4621 atagtcaaca ctgctgtcca gatacgtcag acggctgtcc cagctgatgc cgaagttcaa
     4681 agcggaacac tcccgatcaa tatagatcgg aacgctcaca atgtgatcct gctcgtattc
     4741 atctacgccc acttccactc ttcccagcga aaccgtcaga ttctttttca gcggctccgc
     4801 tgtagtgccg gtcgcggtag ttgtagtcgt ttccgcagta gtcgcagtgg tttcctctgt
     4861 ggtggtagta gtcatcgttg tcgtcgttgt ggttgttgtc gttgttgcag aagtggttgt
     4921 ggtcgaagtg gtcgtggtgg tcatcgttgt ttctgtggtg gtagtttctt ctgtggtcgt
     4981 ggtagtttcc tccgcaccgg taatggtgat cgagccactc tccccctgca tcttgctgga
     5041 ttctccggaa gtgctgatcc agtagctgct tttgagtatg cggaaatcca ccgagaacgt
     5101 atcccccgcc ttggtattct ccggcagctg gtaggtgatc gtgccgatcc aaccggcttc
     5161 ctgctcggta tgcgacagat acacacgcag cagatacgaa gtcgaatagc cgctgctggc
     5221 agaagacgtg atgatcttgt ccccgaaatc gcacttcaca ttcgtcagac tgggattgtc
     5281 aacgacgtta aagttcatat cgctaaaact ctggctgaca taaaggggca tctccacccg
     5341 atagttgttc gcctgcaatt cctccagcga catttccaga tccgcaaatt tcagacacat
     5401 ggtgtcctcc aactcgttgg gttcggtggt ggtcacggtg acattgtcgt ccacggtaac
     5461 agaaaatgtg taagaaagac cgttgactac ggctgtcagc gttgccgtgc cgtagcccac
     5521 agcagtggca gtgttaccgc tgacggatac cacatcggtg tccgaactgc tccacgatac
     5581 cggttcctgt agcgtaaagc ctttccggtt gctgtaccgg atcttcagct gcaaggtctg
     5641 ggtatcgccg taggacatga cactctgcac agaatcgtcc attacagtca catcacttgc
     5701 gggaacctcc agcttctgga tacactcccc ataggtgaag ctgaggctgt ccgagccctt
     5761 gccggaactg aattccgcac aggtggtatt ggcataggtt gccgtagagg tgtagaacgc
     5821 accgataccg atatagcggc tgccaggaga gatcatgctt ctatagtgac cggcggcact
     5881 gctgctgctg tcgatcagat aggactcctt ctcgccgtac cactgctcga taccatgcag
     5941 catattcttc tcacggttcc atgcaagaca ctctccgtga gaagatgtcc ccgccggtgc
     6001 ctgaatggca aagcagcttt ctccgttggt acgcacatga tcttctgtct gggaagcctc
     6061 tgcggcacgg atccgtgcga tatactccag tccgtccgac cactggatcg gctcatagtc
     6121 gctcatagtc aggctgccgc tgccgtcagg gttgggatag ccgttctggc aggcttccag
     6181 ccggtagctg ttgatcaagt ccagtgcctc gctgatctgg tcaatatacg tgccctgaat
     6241 gccgatcagc aggttgccgg aactcccctg cgatacgtcc gtttccaata ccggcagttc
     6301 ttccgccgaa gcaaagctgc ccagcgacag ccctgtacaa aacagcaccg cggagagcag
     6361 caccgccata actcgtgacg gcaggctcct gctggtgttt gcggcatgcg atcgtgtttt
     6421 catcttcacc aaactccttt cgaacatatg gcatattctt ctatgtctat tataccacaa
     6481 gtcaaataat gaaatgtgaa tttttcacga cctttgcaga aaatcccgat ttttggagaa
     6541 atccacaaag aaaaaagccg ccaaaggcga ctttcttgca aaatgtataa atgctggaaa
     6601 ataattttcc attttcggtt agggaaacac tgaataaagc ttgtgcgtaa gatgcgtagt
     6661 cagatttttc ggcagattgc atagaaattc cgcaggcata ctg
//
LOCUS       DAWBGR010000143         6703 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2948_length_6703_cov_3.477587, whole genome shotgun sequence.
ACCESSION   DAWBGR010000143 DAWBGR010000000
VERSION     DAWBGR010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2948_length_6703_cov_3.477587"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1995 a         1387 c         1421 g         1900 t
ORIGIN      
        1 agaaaatttt ttctgttttt ttaattttag gggttgactt ttatttgcag gtcttcctaa
       61 gaacaacaaa aaagcccgat ttgacaatag gaatccgagg gtagacagaa tacagcttta
      121 cagaggtttt cttagactta gcccgtaaaa acgggatgcc ctaataagct gatattccga
      181 gtacctcaag ccctattgca aatcaggctt tttgatgact ctttcgtttt cagtcttgat
      241 gttgtccttg actgtgtcta tatcatacca tagtttttcg taaaagaaca gttaataaaa
      301 acacgagaaa attcttgttg aatatgcttt gtaattggtt gcaattagtg tttgaattgt
      361 ttgttattac taaaaatatg cgtattttac tgcatattcg gcagaaatat acgcattttt
      421 tcgagaaaaa ttcgggtttt aaatccgagt tcatgcaaaa aaaattcagg cagtcgtttc
      481 aactgcctga atgattgatc tatactgctt tttgcgcctt gctgcgtggt ttatatcccg
      541 tagtccatgt cgacttgcca atacttctca aataatcatt gacctgataa acattaaagc
      601 cggggacagt attcttaaac cacagatatg tcttatactc ctcgctgtca atttctgaga
      661 tcagcttggt catgatgagg agttcgtttc ggtagacttc ttccagattc aaagccgtga
      721 agaatgccag catcgttccg aggcgaggtg tgtatttttt cttgctgcgg agagcagtca
      781 gtgtttctac gctgcagtca gcggcttccg caaccgcatc ctccgtcatg ccggaacgct
      841 tcatcaggaa tttgacggtt tcgcctctgg tcatttttcg tgtctgcttg acgccatttt
      901 cttcatactc aacagtcaga aggctgagaa gatattcgtc acggttggct tccagtgctt
      961 tcaactgctc ggcactgaat gccatttcat agaatcgagc ctgctgttcc ttatttagtt
     1021 cgccaaaggt atatcgatag ttttccttac catacacacg gcggaatggc agacagcatt
     1081 ccgccatatt cttttttgct ttttctgtca gccaccagcg accgttgaaa ttgataacat
     1141 agtccggatg attcaggcaa aggtgtccgt ctgcatagac aaagctgcct gaaaagacta
     1201 acttgtcaaa tgtgaggttt gtgccgagta ttttggacat ttcctcaatt ggaagggtgt
     1261 atgtccaatc atctgggaaa tcatccggga tctcatgtgc cttggcatac gacctgtcgc
     1321 cccactcaaa tacacctcga actcgcttga atcccaaatg atgcaagcga ttcttggcgg
     1381 tcgtgcgata ggagccgtat gtttcagtga tatgctcgat cagtctttca tagccgtaga
     1441 ggtcggtatg atgaatatct ctgccaagct catccttaaa attctcaagg gtaatatcaa
     1501 ggttttcctt gtaaactact gcacgggctg agatcgcatt tgcctgtatc tccatgctgt
     1561 gtatcgtgtc ctgctgatct tccgacataa agaactcatc aaaatccgga atttccagct
     1621 ttacaagcga ccgataataa tattgaagct cataaaacag catgtgatac tcaatatgaa
     1681 tgcactcgtg aagtatattc atcagataat cagggtcaca cttactgctg acaagaattg
     1741 tgccgccctc tatcagcttc tgaacagcct tgtcaccttc atatacagtg accattttgg
     1801 tatggaatat cgtcttagct tttacttttc cattccttga aagctgttcc acctgaatcc
     1861 tgtagcccat agcctttaca agactttcta tatcaatttt acacggacct tccaatgctt
     1921 ccggctgata tttcaatagg aagttatagg cgtaagcgtc ataatgagct ttgcgaatat
     1981 atggaacaag acagtcatca agatgatgct tcaagcgcag tcgttcacca cgataaatat
     2041 ccacgccgag aaaataatcc gagggagagt aaaacgcatg gtagccgttg acgcagtatt
     2101 tctgatagta gtcctcaccg gtaacaggat catagaagtg gacatcgcaa atgacataca
     2161 ttgcaaaaga gaagctgtcg agctggtcgt agtaaccttt atcgatcagc acatccacga
     2221 tgacaggctt gctgtaagca ccgaaatacg gtaccggcag tctgccgtgg gcaaactccc
     2281 gtcgaataaa ttgtccgtat tctacaacat agttctcctt gagaatctgc gtaaaggaaa
     2341 ctatctccgg tgctttatag catcggggat ttcgcacttt atatgaaaag cccggacgca
     2401 gcggatccgg ctgcatcaga atgggcccga tttcgtagtt cattcaaaca cctccgttca
     2461 aaaaaggtgt ctcctcataa gttcttccac acggtcacag caacaccttg tatcagacat
     2521 tccgtgacat atattggttc ataatcatca ttttcaggct gaagccgtat tctctttttc
     2581 ttcggctccg gatagtatct cttgagcgtg gcttcgtcac cgatcagagc gacaatgatg
     2641 tcaccgggtt cagcataatt ctgctgacgc acaacaacag tatcgccgtc atcaatatct
     2701 gcatccttca tagaatcgcc gtctgctgtc aggagaaaga actctccgct gccgagtaac
     2761 gatgcaggca gctttacata ttctttgaca gcactatccg caaagattgg cggtccgcag
     2821 gcgatgtttc cgtaaatcgg acagagtttc attcctgctc cgagttgcat ttttcttgta
     2881 gcgtatccct tttttccatt gtgttcgagt ctgccctcat ctgtcatttt gagaaggtaa
     2941 cgccccaccg atgactttga tattcccgta ccttctgcta tttcagcgat ttccggtgat
     3001 aatcctgttt cttcatagta ttcgtcaatg aagttttcga ttgcatccaa atttgcctga
     3061 atagcttccg cagatattcg cattataatc acttccactt attagggatt gcattccctt
     3121 ttcttatatt ttatcacact atacagcaat tgtcaatagg aaaatagaac acttgttttg
     3181 atgtgcggaa tcatgcacat gacatagtaa gaggggatag gaaaaaggat gcacctgaaa
     3241 aagtaaaaag gcatataatt gcggtattat ataactgaaa gctattgaaa aaatctgagt
     3301 agtgttgtat aatataggta tgaaaacacc atcttgagta aacggaggag aaaatgagta
     3361 ttttagatga gatcataggc agcacctatg agtatattga aaaaatgccg aaggaacagc
     3421 gaaagcagta tgggcaattt ttcacaagca aggaaactgc tcgtttcatg gcagaattat
     3481 ttaatattcc cgaaaataca aatgaactgt ctatcctcga tcctggagca ggctcaggtg
     3541 tgctttctgc cgctttgata gaacgtctgc aaaactgtcc caatatcaaa tccgtatatt
     3601 tgacttgcta cgaaacagac gataatatcg tagaactgct tgaaagcaat ctggaataca
     3661 taaaaaacaa cacttctttg aaaatgacgt ttgatattgt caggaaaaat ttcattacat
     3721 cacaagaaga taattataat gggacatttt tagctgatcc tgctccaact aaatatgata
     3781 tggtgatcgg aaatcctccg tataagaaga tacctaaaga tgctgccgag gcattgtcaa
     3841 tgccggatat ttgctacgga gcgcctaacc tttactttct attttcacag atgtcactct
     3901 ttgatctgaa agagaacggg gaaatggttt atattatccc tcgttcatgg acttccggag
     3961 cttatttcaa ggcttttcgt cagaaattcc tgtcacaagg tgcgattgag catatccacc
     4021 tttttgtaag ccgtgacaaa gtttttgaaa aagaaagtgt gctgcaggag accataatca
     4081 taaaggtcaa aaaaactgaa gtaaagcctg aatatattac tgttacctct accaacagca
     4141 atcaggactt tacagaaatc acaacatttt ctgcaccgta taagactgtt atttcaggcg
     4201 acgattgtta tgtttatctt gtaacgaatg aggatgaagt gaacacactt gactgtctca
     4261 ataagtttga tgatactctt ccaagcctcg gtctgaaaat gaaaacagga ctaacagttg
     4321 acttccggaa tcatgaagct ctccgcagtg aagcagagaa acaggctgta cctcttttct
     4381 attcacagca tattcaggac ggaaaggtgg tatttcctat cggtaaggaa aatgaatata
     4441 tcgtcactca gcagcaaggc ttactgcaga aaaacgcaaa ttatctcttt gtgaagcgat
     4501 ttacagccaa agaggaacat cggcggctgc aatgcggtgt atatattgcc cgtaaacata
     4561 ccggttatga caggatcagc acacagaaca agatcaattt tattgatgga ttgaaaggac
     4621 tgtccgagtg cgttgtttac ggtttatatg tgctgttcaa ctctaccctt tatgattcgt
     4681 attatcgcat cttaaacggc tctacgcagg taaactcaac agaggtgaac tccatgccga
     4741 tgccgcctat gacatctatc gaagcgatgg gtaaagagct gataaaagta catgatatgt
     4801 cagaagcatc ttgtgaccgt attttaagga gttatgtatg agcaaaattg aagaagcaag
     4861 aaaaatacta agtgaactta acgtccctaa gaaacagcag gctgacctgt gctgctatgt
     4921 tcttctggca atgagtgata taaaagaagg aatagcatgg acagaggcta ccaataatct
     4981 gataaggata catgatgtgc ttgtattcac aagagaaaat tatggggtcg aatatgctga
     5041 aaacagccgt gaaacattca gaaaacaggc aatgcatcat ttcagaaatg cagctttcat
     5101 tgaagataac ggcaaagcta caaacagtcc taattaccga taccgactga ccgatgaatt
     5161 tctcgcactt attcaaagct atggcactga cttttgggag cagaaaaaac aggatttcct
     5221 tttaaaccat gaatcgctcg taaatatgta tgcttcaaag cgttctatgc agaaaatgcc
     5281 tgttattatc aatggtgcag gctttacttt ctcgcccgga aaacataatg aactccaaaa
     5341 ggcgattata gaggaatttg caccgagatt cgctccaggc tcggaatgtt tgtatgttgg
     5401 cgacacaacg gaaaaagacc tcgttaagaa tgtagagaag ctgtcagggc ttggctttga
     5461 gatcacacta catgataaaa tgccggatgt tgtcctttac tccgctgaaa agaactggat
     5521 atactttgtt gaatcggtaa catccgttgg accgatggat ccgaaacgca tcagagaaat
     5581 cgaggaaatg actcaaaatg ttactgccgg caagatatat gtaacagctt ttcttgattt
     5641 cgccacatac aagaaatttg cagaatctct tgcatgggaa acagaagtgt ggcttgccga
     5701 aatgccggat cacatgattc acttgaatgg cgataaattt atgggcccaa gaaataagta
     5761 aatgcgaggc gatgcaacat ggtaagcaga acttttgatt taggcagctt ggaaacgtac
     5821 aagtacgtcg ttgttctctc agagtacaac ggaaaaatat tactgagccg ccataaagac
     5881 cgcactacat gggaaacgca aggtggtcac atcgaggatg gtgaaacgcc ccttgatgct
     5941 gcaaagcgtg agctgtatga aaaatccggt gcgattgact tcgatataga gcctctctgc
     6001 gactacaggg catggaatga ggacactggg catggtgcga atggtgtagt gttcaaggca
     6061 ataatcaggg aattaggcaa tattcctgag agcgaaatgg cagaggttca gacttttgat
     6121 tctctgccga atgagcttac ttatccggcg attacacccg tcctgttccg ttaccttgaa
     6181 acgggacaaa actctgtggt gcttcatcgg atgaagctca aagccgagcc gttccgcaag
     6241 attggggacg gacttaaaat gatagagctt cgcctaaatg atgaaaagcg gcagaacgtt
     6301 caaattggcg actatattga gtttactctt atggatgata catcacagaa gctgactaca
     6361 cgagttgttg ctctgcatca tttttcgtct ttcaaggaaa tgtatgcttc gctgccgaaa
     6421 gaaaaactcg gctattctgc cgatgaaacg tctgctccag accacatgga cgcttattat
     6481 tcgaaagagg aacaggaaaa gtacggtgca ttggggattg aaattgagct taataactat
     6541 aaatgagcat ccctaaaaaa tgcacaaaaa ataaggacaa cagtcccaaa aagagttata
     6601 atgtacttgt aacctacatt aactcaaagg agtgttgtcc tatgaaaaca agtatacact
     6661 attctgaaga aatttgcaag agattggaag caaaaaaatt gac
//
LOCUS       DAWBGR010000144         6633 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2987_length_6633_cov_5.160687, whole genome shotgun sequence.
ACCESSION   DAWBGR010000144 DAWBGR010000000
VERSION     DAWBGR010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2987_length_6633_cov_5.160687"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1520 a         1795 c         1653 g         1665 t
ORIGIN      
        1 atacttccag acattctgct tgccattcaa gggccgtttt aagttaatga cattgtgcgg
       61 gcaatccccc tacacaaaca acaacaatcc aaaaaagcca ctccttacac aggagtggct
      121 tttttacgtg tgttcactgg ctggacttcg caccattact tccggtggaa cagtcctttt
      181 ttctcgtagg gctcgctctc gtgggacagc agagtgtacc caatgccctt gatctcctta
      241 tccagcatag aaatatccaa cggttcttca ctgatgacaa cggtttctcc gttcttcgca
      301 gaagatgtca cctttttcac gctgcagttc tggcgaacca gatcgttcat gtgtgcctca
      361 cacatactgc acatcatacc gtcaattttc aatgttgtct ttaccatgat cgataccgcc
      421 tttcttcttt tctcagggca gctcctgaac ggtctgcctt tgtctattat ataccctgta
      481 ggggtatttg tcaatagctt gtaccggaaa aattttcggc ggcaaaacat tgtccggata
      541 tgccgcaaaa actgcacaat cctgcttttc aaaaaaacat gttgattttt gtacgatttt
      601 gatgatgcgg atattgccat gcacaaaatg atatgtgcaa tattctccca ctttgcataa
      661 tatgttataa aaataagcag aatttgtggt tgcatcttct gagaaatcgt gctataataa
      721 atgcaatgca attggacctg attcgaattt tccttgattt ttcccatgtt ctccggaaaa
      781 gatccggaaa attcgctgaa cgcaaaggag aacactatga aaattggagt aatcggacac
      841 aattataccc acggtgcaga ttttgtgatg gattatccga agggaccggg ctgttatctg
      901 ttccttttgg taaaatcccc tgcacgattt ttgttgaacg ggaaaaagtt gcgtttggaa
      961 aagaatacaa ttctcttttt caccccgcac accgcctgcc gctatgctgc ggaaaacggc
     1021 acctatacag acgactggtt ctactgcaac atgacaccgg aggaggaact cgccctgcga
     1081 aacgagggta tgccctttga cacgccgatg cagacacaga acgctgcgga actgtcagaa
     1141 ctaatccgga aaatggcata cgaacactat tccgcggaac tgatgcacag cgagatcgaa
     1201 caccagtacg ccaacattct gttcatgaag atccggcggg aatacaacgc cggcgatcat
     1261 cacagcacac tccagctgcg ggacaacaac cagcagctga catatctgcg aatgcggatc
     1321 taccagacac cggagcagtt cggcacggtg gactccatgc tgaattttgt cagcatgagc
     1381 cgttccggct tgcagcacct ttataaaaag accttcgggg tcacagtaat gactgatgtc
     1441 acctgtgcaa agatgcagcg tgccaaggaa ctgctgtcct ccacggggct gacggtgggc
     1501 gagatcgcgg agcagtgcgg ctatgggtgc acctacagct ttttacgcca gttcaagaag
     1561 aactgcggca gcacaccctc ggacttccgc aagcaggggc gtgtacaggt gcgtatggca
     1621 acttctgcat aagacgactc tgacgggtga aacaccagcg gcatggaccg caaggaagtc
     1681 ataatgatga aaaaaatatt tgccggagca atggctgccg gtattctctg gtgcagctgc
     1741 accgcttctg tgtcggctct gccccagaag cagtcctcca tgcgggacat caccactgcc
     1801 cagctggtca aggacatggg cattggcatc aatctgggaa acacctacga atcttgcggc
     1861 gactggatcg cccagtgggg ggacggcaca ccggaatcct atgaaaccgc gtggggcagt
     1921 cctgtgatta cccagcagat aatccaaggt tacgcagatg ccggctttga caccctccgt
     1981 gtaccggtgg cgtggtcaaa tatgatggag gataactata ccatcagcga caagtacctt
     2041 tctgcggtgc aggaagtggt ggactgggca atagacagcg ggctgtatgt cattctcaat
     2101 ctgcactatg acggcggctg gctggcgaac ttccccacgg acaaggaaaa ctgcatggag
     2161 aagtacaagc ggatctggac acaggtcagc gatgcattcg ccgactacag cgactatctg
     2221 gtttttgaat cgcaaaatga agaactgggc tgggaaagcc tttggaaccg ctggggcggc
     2281 accgagggaa agacggactc ctatgatctg gtcaatgaga tcaaccagac gtttgtggac
     2341 attatccgtt cctccggcgg caacaatcct cagcgacacc tgctcatttc cggctataac
     2401 actgatgtgg aactgacctg tgacagcttg ttccagatgc ccaacgatcc ggcgggacgc
     2461 tgtgcggtat ccgtacacta ttacacaccc tccggcttcg ccattctgga agaagatgct
     2521 tcatggggca agatgcgttc cacttggggc acagacgacg actacgccga actgaaccgg
     2581 aacatggatc tcctgaaaac cacctatgtg gataaaggaa tccctgtcat catcggcgaa
     2641 tacggctgcc cgaaaaagaa caaggaagaa gaatccgtcc ggagatttct ttcctcggtc
     2701 tgcgaagccg cctattcccg cgacatgtgt ccggtaatgt gggacgtcac cgatttgcac
     2761 tacaaccgga gcagctgcaa gatgtttgac gacacactga tgcagcagct gctggcagtg
     2821 aaacagtccg gtgaaactac gctgacaggc gactgcaacg aggatggtat tgtcagcgta
     2881 gcagacgcgg tactgttgca gcagtatctg ctggacagca agtccctgtc ggaaaccgct
     2941 gcaacattgg caaactgcaa cggcgacggc agcgtgaacg gactggatct ggcagtgctg
     3001 cggcaaaagc tgacagcgta accatgacat tttcagcgtg catctttctt ctccagaaag
     3061 atacgcgttt ctcaaaatgt atttcacagt ttgaatccct gtgaggtaca cacattctgc
     3121 aaaaagcgga cgttcttcca actggaaaaa tgtccgcttt ttgtatttgc agaacagcca
     3181 tgccttgtgg aaaagcagct ttaccagcat gatttccact gcccgttttt ccacagaagc
     3241 tgtttccacg ttttccacat gtttcaacaa ttacactttt ccataaaaga gaaaaccggt
     3301 tgaaaacatc gcagtttcaa ccggttttcc gattttcaat tctatttttg ttttccacac
     3361 acgcaatttt tgctgtggaa aactagggaa acactgaata aagccgtcag gcgtgctttc
     3421 ccctaggtga aatatgctct tagtcctgcg gacgaatgca gtcagtgaac atgtaccgct
     3481 gcaggggttc cggaatgcgg acagagccgt cctcgttgag gttgttttcc aagaatgcga
     3541 tcagcatgcg gggcggagca acaacggtgt tgttcagggt gtgggcaaag tacttgttgc
     3601 cgtccttgcc cttgatgcgg attcccagcc gtcttgcctg tgcgtcgccc agattggagc
     3661 agctgcccac ttcgaaatac ttcttctgac ggggcgacca tgcttctacg tccaggctct
     3721 tgactttcag gtcagccaga tcgccggagc agcactccag tgtccgaacc ggaatgtcca
     3781 gactgcggaa gaactctacg gtatagccgt acatcttatg gaaccagtcc atgctttcct
     3841 ccggcttgca gacaacgatc atttcctgct tttcgaactg gtggatacgg tacacgccgc
     3901 gttcctcgat gccgtgtgca cccacttcct tccggaagca cggagaatag ctggtcagag
     3961 tctggggcag cttttcttcc ggcagaatgg tatcgataaa cttaccgatc atggagtgtt
     4021 cgctggtacc gatcaggtag aggtcttcgc cctcgatctt gtacatcatg ccttccattt
     4081 cggcaaagga cattacgccg gtcaccacgt tgctgcggat catgaacggc ggaatgtagt
     4141 aggtaaagcc cttgttaatc ataaagtcgc gggcatatgc cagaatggag gagtgcagcc
     4201 gtgcaatatc gccgcacaga taatagaagc cgttaccgct ggtgtcgcgg gctgcgtcga
     4261 tgtcgatgcc atggagtttt tccataatgt ccacgtgata cggcacttcg aaatccggca
     4321 caaccggctc gccgaaacgc tctacttcca cgttttcgga atcgtcctta ccgatcggca
     4381 cggagtcatc aatgaagttc gggatcacca gcatgatctc gtggatacgg gcagacagtt
     4441 ctgtttctgc cttttccagt tcttccagct tggtgccgat ggcacctacc tctgccttga
     4501 cagcttcggc ttcttccttt ttgccctgtc ccatgagggc accgatcttc ttgctgatag
     4561 acttccgctg gctgcggagt tcgtcgccct gtacctttgc ttcgcggaat ttctgatcca
     4621 gttccacaac ttcgtctacc agcggcagct tttcgtcctg aaacttcttg cggatgtttt
     4681 ccttgacttt ttccggatcg ttccggatcc atttcatatc gatcatgatt ttttccttcc
     4741 tttatgtatg tgggatgccc acaagattac agtatgtgta gaggcgaaca atgtttcacg
     4801 tgaaacaccc tcgccgcctt actctttcgt cagcttctgt gccagtgcag acaaatcgtc
     4861 tttatcgtag aattccagat tcagtgtgcc cttgtttttg ccggcggtca ctgtgacacg
     4921 ccgtcccaga cggctttcca gactgatctc gatctcctta tagaagctgt ccttatggct
     4981 gaacttcgtc cgtttttccg gcttttcgct gtcggcagcg atcttttcca cggcacggac
     5041 agtcagcttt ccgtctgccg cctttgccgc cgtttcagag agcagcttct cgtcagagat
     5101 gcccagcagt gcacgggcat gtccggcact cagtccgccg tcctccacca gtgttttcac
     5161 atcttccggc agcttcagca gccgcaggga attcgccacg gcactgcggg attttcccac
     5221 acgcttcgcc acttcctcct gcgtcaggtg aaacatgtcc atcagctgtg cgtaggcgtt
     5281 ggcttcttcc agtgggttca ggtcctcccg ctggagattc tcgatcagag ccaccgccat
     5341 ggtttcctcg tcggtcagtt cccgaatcac caccggcact tcgtccatgc ccagcattct
     5401 cgctgcacgc cagcggcgtt cgccggcgac gatctgataa ttcagcccca gcggacgcac
     5461 cagaatgggc tggatcatgc cgtactgctg aatggaatcc gccagcgtct gaatggcttc
     5521 atcgctgaac tgtttgcggg gctggtcccg attgggttcc agttccgaaa cgcggagcgt
     5581 ctttttcacc tgcacctcgg tggcattttc atcatagaga aaatccagac cgctgcccag
     5641 tccgccgatc gtcattggtc accactctcc cttttcttgc cgaacgacaa cagccgccgt
     5701 tttttctccg gcggtttttc tcccagcaat tccagaccca gcagcatata cgcctctgcc
     5761 cccttggaaa accggtcgta atacatcacc ggtttgccgt ggctgggggc ttccgaaagc
     5821 cgcacgttgc ggggaatttt cgtctgaaag accttgttcg ggaaatactt tttgacttct
     5881 tcttccacct gtcgggacac gttgagccgc tggtcaaaca tcacgaacag aatgccgccg
     5941 atgtccagat tggggttata gttgctgcgt accaccttaa tggtgttggt cagctgggac
     6001 agaccttcca gtgcgtagaa ctctgccagc atgggaataa tcagctgatc tgccgccacc
     6061 agtccattga cggtgagcag ccccagtgcc ggcgggcagt cgatcaggat atagtcatag
     6121 tccagtttac aggtcagaat ctgcattttc agccggttca tgcgattgtc catggcggac
     6181 agttccagtt ccgcaccggc aagggtgttc ttcgccggca gcaggctgac gttttcaaac
     6241 gctgtgcgga caatggcttc ctgtatgctg cacttcccca ccagcacctc atatgaagaa
     6301 accggcagct gttttttctt gatgccaaga ccggtggtgg tgttgccctg cgggtcgatg
     6361 tccacgcaga gtacacgctt gccgcgggaa cccagatatg cccccagatt caccacggtg
     6421 gtggattttc ccacgccgcc tttctggttg gcaactgcat acacaatact cacgcgggtt
     6481 ctcctttcct gcggtgtgtt gaaaacgagt tgtcccagca caccgaattt gattgaaagt
     6541 atgtttgagg caacaccgca caaagcttgt gcgtaaggtg cgtagtcaga tttttcggca
     6601 gattgcacag aaattccgca ggcagactgt atg
//
LOCUS       DAWBGR010000145         6612 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_2997_length_6612_cov_7.959738, whole genome shotgun sequence.
ACCESSION   DAWBGR010000145 DAWBGR010000000
VERSION     DAWBGR010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2997_length_6612_cov_7.959738"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3887..3896
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1557 a         1664 c         1714 g         1667 t
ORIGIN      
        1 aaaaatttga cgacgcatct gtcgcacaat ctttgtgcgg tattgcccta tgaatgtatc
       61 aaagcggtta catacgtcct catttcaagg acggtgtgac cgcttttcat atatggaata
      121 ggttctatgg ttggcgacaa gcaggcacag caaaaccggc tctgcacgga gatttccaca
      181 cagagccggt tttgtttatg attcaaagga ttttatgaag tgggtgtctt acttgataat
      241 gtcttcccaa gtcacattca cgcctgcacc cttgcttgcc acatagtaca gggcatagaa
      301 tgcatcggta gaatctacgc tgccgttgcc gtccacatcg gctgctgcga tcatctgctt
      361 gttcttttca gcgtcttcgc tcagaacaaa gtcagcgttg cctgcggaga tgtttgcggt
      421 gtacagcatc atatagaaga cgtcagtaga atccactctg ccgtccagat ctacgtcggc
      481 tctggagtct gtcacttcga tttttacttc gaaagtcagc ggtgtggtat aaccagttgc
      541 ttccacagtc agcttgtatg tgccagactt tgtaaagact gcaccgtcac gggtttctgc
      601 atctgcatga atttcgccgg tggtcgggtc aatgatcttg acaccgtgtt tgccggttgc
      661 ttcatatgtg gtatcgttta cagtaacctt ggaaacattc ttcagatatg cagccagatc
      721 gtctgcggca gcaccctttg caggaacaac agcacccttt tctgcatcat atgcagcagg
      781 cattgcatcg gtggtcagga tcacgctggt agagagcggt gcatatacgc cggattcatc
      841 cgaaacggta atggtatagc tgcccggcag aacgctcttg gcatcaaagg tcatcttgcc
      901 gtctgcataa gcagcaccag tcagcgtgtc tgcggaaacc gctgcggaga aatcttccgg
      961 caggtctgcc agcgttaccg gaatgctgcc ggcagcaact gcaacgcttt ctgcgttgac
     1021 agtaccgttg tgcttcttcg gcagatacag tccgtctgcg aggtgtacgc tgtattcgcc
     1081 cttgctggtc agatagctga tctccacaat ggtgtcaccg gaggttgcag catacggagt
     1141 agaacgcagg ctgtttccgt gtacttcttt ggtcttgaag ccggcagacc atgccagttc
     1201 agagccgccc ttccagatgt tttccagctg gagcatacca tagttgccgg tctttgtctt
     1261 gacgatcacg ccgtaaacca gattgccttc gcccagatcc agaccgtcca tcttcagctg
     1321 atagtcaccg tacttgctgt cggttttgag agtcggcgtg acgttggtca ggtcaacttc
     1381 cggttctgcg gtaatggttg cggacagggt gccgtctgcg aacagctgtt tgtatgctgc
     1441 cggaacaacg gtctgatctt cgttcggggt gaagcctgca aacaggctgc ccagtacgcc
     1501 ggaagtcttg ctgtcctttg cgtcagcagt cagctggtcg tacaagctct gttcgattgc
     1561 cacttctaca ccggaaacgc ccagaatctt gccgccgcca ttttcatcag cttcggaaga
     1621 atacgtggtg tctgcaaaca tgctgttcca ctttttccct gttgcagagg tcactgcatc
     1681 ataagtgccc tctgcacgca tgccggcatt gccagccaga tctgcatcat aggaaatctt
     1741 gccgtcagtg gtatctgtct tgttgcccag ttcaccgtaa tagtaatctg catacgggag
     1801 attcatagta ccgtaaacat actgttccgg ttcgttgtcc actgtgaatg taaagtcctg
     1861 ataaccggta gcataaaccg taacgtccca tctgccgtag ccgtcagtac cctccggcag
     1921 ctggcagcgg tcgctcttgg tcagaccaag ctgaatgccc atggacttgt gcatccagtt
     1981 gtctgcggca aacttggtgc cgtaggtagc cagaacggtg tcaccatcgc cgtagtaatc
     2041 ccagcggact gcgtacatat gagcacccag atcgccgtaa tctccgttga tgtccacgcg
     2101 gataaagtca ccgtagccgg aatcgtgctg gacttcgctg atctctaccg cgtccttgtc
     2161 taccactttt ccatccggtg tttcagaggt ggaacgcttg gagaactgga atgtgccgtc
     2221 ctcctgtttt tctgctacct tgatgccgga agtgttggca tctacctgaa tttcttcgtc
     2281 gatattgacc agattctttt cggtatagcc gcccttgact tctgtcccgt tttcatagac
     2341 cgtataggct ttcttgaagt cctcataatc tgctgccgga acagcaaccg gaacatattt
     2401 cggtcccacg atcacagagt ggtcgtatgc accagccgct tttccgtcta ccataacggt
     2461 tgtggtacca gcatctctgt tgctgaccac atcgacagat tcgttctgtc ccctgccggt
     2521 atagtcgtag ctgtttcctt cggtatcaaa tacctttacg gtgcactgat agctgccgcg
     2581 gtgcagaccg tggttgctgg tggcacgggt gactgcatca tatgcaccca agtcggtttc
     2641 tccgtgggaa tcggcatcag cagaagaagc ctcccagttg ccgtctgcgg cactgtccag
     2701 ataaacgcct tcattctccc aatactcagc ccaagtcaga gatgcggcac agtacacata
     2761 atctgtggtg cctgcttctt cggcatacgc tgtgatcgcc ggagcgacct gtgtagctgc
     2821 cagtacagca gcagacgctg caccgacaag ctttttccag ttgatctctc gcatagtaac
     2881 ctcctgaatt tttcttccgt gctgcattgc gatgaacagc acaatttatg gcaataccgc
     2941 acaaagattg tgcgacagat gcgtcgtcaa atttttcgtc agatgaaaca aaaagcggag
     3001 tgaatacttg atgtattcac gagcattttt gtgaaatatg acgagaaatt tgcaagcaga
     3061 tggcgtgcaa agtcgccaga cgctctttgt gtggtattgc cttatagcaa atcctgcaat
     3121 tttgcaggat acactaccca ataggattta caattagccg taactaatta gtttaaacta
     3181 acttaacaaa taagaaataa ctggaatcaa aaattccagt ttatgagtgc tttttgtttt
     3241 tggcaggttt ctctgccggc ttcggaaaaa tagatttcgg caaaaagtga atgacacggt
     3301 ctgccgcaat gccggtgaag aaaccggtaa tgcatcccgc caccaacagg aacgggaagt
     3361 aggcaataac ctgtaccgac tgcatcgccg caattgccgc aagaatctgt cctacgttgt
     3421 ggagcacggc accgacaatg ctgatgaacc acaggtgctt tttttgcagc agcttgcaga
     3481 gcagtgccat gccgagaaaa caaaggattc cgccggtgag ggaatagagc aacgacaccg
     3541 gcgtaccggt gaatatggaa cccagcagga tccgcagtac cagtaccgca aaggcatctc
     3601 gcgggcggta gacaaccagc acgatcaggg taatgacgtt ggcaagtccc agctttatcc
     3661 ccggaatggg aaccggtacc ggaatctgtg cctccaccac aaagatcgtc agagccattg
     3721 tggtaaagag ggcaagcatg accagatgct taacggactt ggacatcgta agacccctcc
     3781 tcgtcagatg caatggaaat gcgaatgtgg ttcggcagac agacgatggg cagaatgccg
     3841 tccgagatcg ctccctgctt gacacaaagg tgatcggggc aggtggnnnn nnnnnngcag
     3901 gtggcagact gcatggagat ctggtttggt tccaccagaa tgacattttc cgcaccgttt
     3961 tccccgtcaa tggtgagtgt gtaggatttc ttgaccgtgg aaaggtcgat ttcatataat
     4021 aagttgtcct gctgatagat gcgggcgatt ttttccggct gcttgtggtg catgacatac
     4081 aagctgccca gaatcccgcc gcccagcagt acaacggtaa tgccaatgag aatcagcgtt
     4141 tttttcatga attgtttacc tgtaactgat acttgtcgat gtgcttcgag gtgacaacag
     4201 agccgtccga ctgaatgaag accgcttcaa tgccgtccag cgaatcgatg agctgcatgc
     4261 cggcatctgc ccccatgaga aatgtggcag tggaaagtgc atcacagtca atgcctttgt
     4321 ctgcaataat ggtggtgctg accacgtcag attccgacgg ggcaccagtg cagggatcca
     4381 aaatatgatg atagcgtttc ccgttttcct cgaaatattt ttcatagttg ccggaagtga
     4441 caacagcctg tccggtgacg gtgacggaac ccattaagct ttccggcgag aacggatccg
     4501 taatggcaac tgtccagctg ctgccgtcgg gcttggtacc cagtgtcatg acgtttccgc
     4561 ccagactcat cagggcagag gtgacacctt cttcttccag cacctttttc atttcatcgg
     4621 agatataccc ctttgccact gcaccgaagt tcagtgtaac ggcatcactg gaaaaggaga
     4681 gggaactgct ctgtacatca tagcggacat cgctccagcc ctccggtcgg agcagtgcct
     4741 gtatgcggtc gtctgcggga atggcagcgt ggtcggtgcc gatgccccac aggtcgatca
     4801 ggtcgccaat ggtgcagtcc agcatgccgc cgcttttttc gccgtattcc aaagccgcat
     4861 acagcacatt tcccaggtct gcggaaatag ggatctctgt tcccacagca gcttttgccg
     4921 ccccattgac cttgttgact tcggaattcg caatggtgtt ggaaaagacc tgttccagtt
     4981 ccttggcacg ggcaaaggca gcttctgcac cggtctgggc gtgttccccg taggtgcgaa
     5041 ccatgatata agtgcccata aattcctgcg tatcttcata gactgcgtcc gcggcagaac
     5101 tgtctgtgct gtctgccgct gtgcctgttg atgcggaagt gctgtccgaa gatgccgtac
     5161 tggtgcagcc cgtcaggcag agcagtccgg caagtccgat tgcccatagg tgcagatgct
     5221 tcatgctgga aacgattcct ttccgtagaa tggtagttag tatcttctaa cagataccgg
     5281 atttagtata gcatgctttt ttcactctgt caagtccggc ttgaaaagat ttccgcaacg
     5341 ctttttgaag taactgtaaa ccgaaaaaaa tcttttctca accgcagaaa aaaattgtgc
     5401 aatatatatc ggacagcatt gcgaattgct gccatgacag gcttgcaatc tataaaaagg
     5461 tgtggtacaa tagtacaata aaggaaacat ttgtgtgaac atatttgggg cagcactgca
     5521 caaaacttgt gcgtaaaatg tgtcggcagg cgaacctgct gccgtgctgc ccgatgcatt
     5581 caggaggttt gagcgatgat ctatacagtg acttttaatc cggcaattga ctatgtggtg
     5641 cataccgatg ccatgcaggt gggtatggtc aaccgttccc gtacagagga gatctactac
     5701 ggcggaaagg gcatcaacgt gtcaatggtg ctgaaagaac tggggctgga atcccgtgca
     5761 ctgggctttg tggcaggatt tactggtgcg gcaattgaaa agggtgtgca ggcaaagggc
     5821 attgccaccg attttgtgca tttgcaggag ggcttttccc gcatcaatgt caagatcaag
     5881 tccggagaag aaacagaact gaacgggcag ggacctgtca tcggggcaga agcagtcgcg
     5941 gaactgttcc ggaaactgga taagttacag gacggggata ccattgtgct ggctggcagc
     6001 attcccagta cgatgccgtc ggacacctac gaaaagattc tggcacatct gaaaggaaaa
     6061 caaattcgaa ctgtagtcga tgccaccaaa gacctcctga aaaacgtgct gcaataccag
     6121 ccgtttctca tcaagcccaa ccattacgaa ctgggagaac tgttcggagt cacgctgcac
     6181 agtcacagtg agatcgaagc ctatgcccga aaattgcagg aaatgggtgc ccgaaatgtg
     6241 ctggtttcca tggcggaaca gggggcactg ctgctggacg aagccggcat ctgccatatc
     6301 tgcggggtgt gtcagggcac tgtgaaaaat tctgtgggag ccggcgactc tatgcttgcc
     6361 ggttttcttg ccggtctgga aaccggagat tataccgatg ccctcaagct gggcactgcc
     6421 gccggcggag ctacgacttt ctcggagggt ctggcagaac gggcagagat cgaccggttg
     6481 ctgctgcaat tgtaaaaaat atgtgaatcg cccagaatta tcgtatttga aaaactgatg
     6541 tgttatgata ggtttgccaa ggcaataccg cacaaagatt gtgcgacaga tgcgtcgtca
     6601 aatttttcgt ca
//
LOCUS       DAWBGR010000146         6598 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3005_length_6598_cov_2.939936, whole genome shotgun sequence.
ACCESSION   DAWBGR010000146 DAWBGR010000000
VERSION     DAWBGR010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3005_length_6598_cov_2.939936"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    560..569
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4257..4266
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2076 a         1217 c         1577 g         1708 t
ORIGIN      
        1 tcaataacaa catgaaggat gtgcgtgtcc actactatgt ggataacgtg tgtgcgtggc
       61 agaatctgcc ccatagtctg agcggctggc acgccgctga tggcagtggc aatggaaatc
      121 gcagaaccat tgccattgag tgtattatgt cctctgcgta caattctgtt gataaaaagt
      181 cggaggacaa tgcagcgaaa ttggcagcag cccttctgaa acagtatgga ttggacatca
      241 accatctcta cacgcatacc cactggctca atgttcgtga cggacgaaac ggaacgattg
      301 accagctgaa caccatgtac aatcggtaca agatgtgtcc ggcgtatatc ttgccgcatt
      361 ggacggagtt caaggaaaag gtacagtctt atttgaacgc aggaacttcc actatttctg
      421 caccttctac aaagcagctt taccgggtga gaaagtcttg ggcagatgcg aagtcgcagc
      481 ttggtgcgta ttcctctttg gagaatgcga agaaagcctg caaggtcgga tattctgtat
      541 ttgatgccaa cggaaatgtn nnnnnnnnnc tcaaaaatcg gcattctttc tctccgattt
      601 acgtggactt attgccgaaa acgcttgcaa ttccgccgaa aaagaggtaa tatggacacg
      661 tcaaaaggag gtgcagaagc atgaaaacta ttactaaaat tgaggcaaat cgctctgcag
      721 ctgttcatcg aaaatgtcgt gtagcggctt attgccgtgt ttccacagag catgatgacc
      781 agatagaaag tctgggaaca caaaaagaac attatgcggc gtggatcaaa ctgcatacag
      841 agtgggaatc tgcgggtatc ttttatgatg ccggcattac tggaacaaaa gcagaaattc
      901 gtcctggact gcaagacctt ttacaggctt gccgcatggg cagggtagac cgcattctgg
      961 tgaaatccat cagtcggttt tccagaaata cggcggagtg cctcgctctt gttcgggaac
     1021 tatcaggaat tggggtttcc gttttctttg aaaaagaaaa catagacacc ggcagtatgg
     1081 aaagcgaatt gtttctgacg atactcagca gcatggcaga ggaagaatct ttatccatat
     1141 ccagaaatga gaagtggtcg gtacagcacc ggtttcaaaa cggtacctat gtgtcatcgt
     1201 ctttccctta cgggtattgc agaaatgaca ggggagagat ggtgctcgaa cctgaggagg
     1261 cagaaattgt gaaatacatt ttttctgcct tgttattcgg aaaaagttct tgtcagattg
     1321 cagatctgtt ggaacagcag gggatccctt tcaagaatgg acgtcattgg tgtgatgctg
     1381 cgattcgtgg aattgctgcg aatgaaaaat atgtgggaga tgttttgctg cagaaaacgt
     1441 ataccgatgc acattttcat cggcacaaaa atcatggaga agtggaatgt tatcttcttt
     1501 cagatcacca cataccaatt gttagtcggg aaatttttgc aaaagcaaat gcagtcattc
     1561 gacagcgagc tgccgaaaaa ggcattgtgt gtggtacagg aaagtatcaa aagcgatatg
     1621 ctttttccga aaaggtgatt tgcggcaaat gcggcagcac ttgcaaacgc aggatccaca
     1681 gcggcaatga aattgcatgg acgtgtgcgg ctcatattga aagtgctcaa aaatgtccta
     1741 tgaaatatgt gcgggaggat atattgaaag ccgcttttgt tacgatgttg aacaaactga
     1801 ttttcagcag aaagcacatt ttgaaaccat tgttagaaca gctgaaagcg aacagcaatg
     1861 atgaaaatgt ccggcgaatg caggaactgc aaaagcagct ggaatctcat gctgaaaaga
     1921 aaaacacact gcaccgtttg tatgcacaaa aggtcataga tcctgtttta ttccggcagg
     1981 aaatgaatgc tttgcagaaa caagcggagt cctgccgtat ggaaattgca cagttggaac
     2041 aggaaacaca tggagaaact gagataattg cagaattaaa gcagctgctg cgatttacag
     2101 agcagcattc tgcaatgttg acagaatttc aggagacatg gttttctgca tttgcagaac
     2161 aaataatttt gtatgatcgg aatcatattg gatttcggct caaatgcggt ctgctgttaa
     2221 aggaggaaat ttgatgggac agattcctta cggctaccga attaaaaacg gtgctgctgt
     2281 gattatatcg gcagaggcag cacagattcg ccttattttt caaaattata ttgccggtat
     2341 gagtttacag tcggcagcaa gagcagcagg tcatcccatg gcacatagca ctgttcgtcg
     2401 aatgctgcag cgaaaatgct accttggaga tgctttttat ccggcaattc tggacaaaga
     2461 aacttatgct cgggcaaatg cagagtggca gcatcgtgca gatgtaatgc agcgacttgg
     2521 aaaaacgagg agaaagcccg tatgtccaca gacaaaattt ttgttggaac tgccgcagca
     2581 aataccagaa ctagatagaa atacaccatt tcagcaggca gaatatcttt atcatttgat
     2641 acaaaacaag gagtaatgca acaatgccaa aggtcactac aattccacca cgaaagcaaa
     2701 gaaatcatgc tgtcacgtca caggaaaccc ggaggatccg tgtggcagcc tattgccgtg
     2761 tttccacgga tacagaggaa caggcaacca gctatcaggc acaaattgca cattatgagg
     2821 aagtcattca tagaaatccg gaatgggtct ttgctgggat ctatgccgat gacggcatca
     2881 gtgcaacctc tacaaaacat cgggaacagt ttcatcagat gattcaggac tgcatggatg
     2941 gaaagattga tatgctcatt accaaatcca tcagccgatt cgccagaaac acagtagatt
     3001 gcctgaatta catccgacag ctgaaagcac aaaacattcc aatctatttt gaaaaagagt
     3061 ccatcaacac aatggatgcg aaaggggagg tgctgattac cattatggca tcactggcac
     3121 aacaggaatc agaatctctg agtcagaatg tcaaactggg aatgcagtat cggtttcaac
     3181 agggaaaggt gatggtcaat gccagctgtt ttcttggcta tgataaggac gaaaacggag
     3241 atcttgtgat caatccggaa caagccgaaa cggtaaaacg aatctatcgg gaatatctgg
     3301 agggagcaag ctgtcagcag atcgcaaggg gactggaacg ggacggtatc cgaacagcaa
     3361 gagggtatac ccgatggcat gacagttcga ttcggttaat tctggaaaat gaaaagtata
     3421 tgggagatgc tcttctacaa aaaacatata ctgtggattt tctcaagaaa aaacgcatta
     3481 aaaataacgg cgaaatgccg cagtattatg tggaggacga tcatgaggcg attattccca
     3541 gggcattgtt cttacaggtg caggaggaaa ttgcaaggcg cggttcacag gtggactgta
     3601 tgggcagacg gcgtggattt agtgcaaacc actgttttac tggtttgctt tactgtgctg
     3661 aatgcgggga acaattccgc agaatccatt ggaataaccg aggctgcaaa tctgtggtgt
     3721 ggcgatgtat gaccagattg gagaaaaaag gagcgtgtca tgcacgaaca gtctatgagg
     3781 aatctttgaa acaaaccttt gtggatgctt tgaatcaact gacaggaggc agtgaaacat
     3841 acctttctat cttacaggaa aatatggctg aagtgattga aatggaacaa tccaatctgc
     3901 ccgaggaaat acagagaaaa ttagatgttc ttcagaaaaa gttgatcgaa tgtgcagaac
     3961 ggcatgagga ttatgaggag atagcacagg aaatctttcg gctgcgagag caaaaggaac
     4021 aggctttaag ggaaaatgtt tctcaacagg agcagaaaga ccgtatgcgg gaactgcagg
     4081 aatttttggt tgctcagccg catcacatta ccgaatttga tgaaacactg gttcggcatc
     4141 tacttacaaa agtaacggtt tcttccgatc gactggattt tacatttcaa tcaggtgtca
     4201 cggtttccat tgaaaagtga accacttcaa aaatcctcct ttgcaaaata taaaagnnnn
     4261 nnnnnngggg aggattttta gattttataa cggcattgtt gtcttgattt ctccttaaat
     4321 ttatggtata ctaagaaaaa acggaggtgc tgcatcatgg gaatctatct gaacccagga
     4381 aatgatttgt tttactctac agttacttat tctgaaattt atgtggataa gaccatgttg
     4441 atttctttca ccaataaatg tttgtttgga gaaaacaagg agatctgcgt cagccgtccc
     4501 agaagattcg gaaagtcgat ggcagagaat atgctgacgg cttattacag caagggctgc
     4561 gattcgagag aattgttttc caagtttcag atcgcacaga caccggattt tgaaaagcac
     4621 ctgaaccggt ataatgtgat tcacatcgat atgcagaaat tccttggcag aaccaaaaat
     4681 gtccatgaaa tgctggactt cttgcagaaa cgtgtgctaa aagagatgaa acagacattc
     4741 tccgtgatag agccggaaga aaccagtttg attattgcgt tggaagatct gtacggtcaa
     4801 tgtgaagaaa agtttatctt catcattgac gaatgggatt ccattttccg agtacaccgg
     4861 gacaatgcaa ctgctcagaa ggagtacttg gattttctgc gggatcttct gaaaggacag
     4921 ccctatgtgg cacttgccta tatgaccggc attctcccaa tcaagaaata cggtcaacat
     4981 tctgcactga atatgtttga tgaatactct atgaccaatc aatatgcact cgcagaattc
     5041 actggtttta cagagaggga agttcgtcag ctctgtgagc gttatcatat gtctttcgag
     5101 caaacgaaag attggtacga tggctataat gtcaatggtg tatccattta taatccaaga
     5161 tctgtaacat cagcgatgat gaacggcatc tttgacagtt actggacaca gacagaaacc
     5221 tatgaggctt tgaaaatgta tatcgttcgc aatgagaacg gtttgcggga taaaatcgtt
     5281 cggatgattg ccggagaaca tatttccatc aatacgaaaa catttcagaa cgatatgtgt
     5341 acttttgaaa ctgcagacga tattctgact ttgctggtac atctgggcta tctgacttat
     5401 gacttcgata caaaaactgc ctggattccc aacaaagaag tgcggcagga atttctcaat
     5461 tctatccagg gacaagaatt tcagaacgtc aacaatgcca tccatcgttc cgacaagctg
     5521 ctgcaattga cgttggcaca gaatgcggaa aaagtggcgg aaatgcttca ggaagttcac
     5581 tgtgacaact gttctgtaat tcagtataac gatgaaaatt cgctggcttg tgtgctgagt
     5641 ctggcatact attctgcaca agacagctat gcggtttatc gggaattgca aggcggagaa
     5701 ggctttgcgg atctggtatt tgtaccgaga actgggaatc ataacccggc aatgatcgtg
     5761 gagctaaaat ggaatcaaac cactggtatt gcactggaac aaatcaaaga ccgaaagtat
     5821 attcgctgtt tgaaagacta tcatggaaaa gtgctgtttg ttggtgtgaa ctatgataag
     5881 aagagtaaga aacatacttg tcagtttgag atgatggaaa tttgaaaaac tctatagaca
     5941 gacttaacag aacatatatt gaatgcaaat attagggtga aagccataaa aagcacaagc
     6001 aaatccacta ttaattgtca catcctaaac ttatgaaagg tactcgtgta tttgctaaaa
     6061 cttacagtag attagtagct atatatttgg tagactaaga aggattaaaa tatgggaagc
     6121 attattgatt ttagtggtat agaaaattgt gaacaagcca tcagaaaaac atgggatgaa
     6181 tttagggaat gtttagcagc tggtgtattt tcttatgatg aaattgaaaa agctaaaaag
     6241 cagaaatttt taatggtata tgatgatcta ttttataaac attctggtat tgaccaaagt
     6301 acgattgttt ttaaggattt gaaagataag ctaatgggaa gaggtgctat attgaaaaat
     6361 gatgagattc ctaattatga tcgttttctt cctaaagaag agtatatcaa agaagataat
     6421 cgttttagcc ctcctcatgt ggaatggctt tatttggcgg ttggcaatga taacgacatt
     6481 cacgaatgtg cacaggctga atgtcgagcc aaaagaggaa acatgtttgg attttgtcat
     6541 tttcaacgag acaccaaata tgatatgtgc aaattagtag atctaaccat tgcagacg
//
LOCUS       DAWBGR010000147         6558 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3025_length_6558_cov_3.422728, whole genome shotgun sequence.
ACCESSION   DAWBGR010000147 DAWBGR010000000
VERSION     DAWBGR010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3025_length_6558_cov_3.422728"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1605 a         1576 c         1418 g         1959 t
ORIGIN      
        1 tgtcgcacaa tctttgtgcg gtattgcctt agagatactg atcgcattgt gaatcgtgct
       61 ttcgtcatag gcgatcggca caccgtgatg ttttcccagc atgctgtgca aagcgtttgc
      121 cgttccgtag aatgctgtaa agacgttttc gtcgtccggt gaactgtaca aagaggctgc
      181 aagctttaag gcggtcgttt ttccggtaga ggaagttccc accagtgaca cgccaaaaca
      241 atccgcaggc agcttttctg tgatcgtcag gtaggcaagc agtgtggatg ccgctgctgc
      301 acaaagtaca aactgcagtg acggcatagg gagtatcaga cggttcagtt ctgcaaggta
      361 atcctccggt gacatctcca gatgtgcccg cagcacaggc gggctgtgag ctgcaccgca
      421 ccagtgcagt gtattgctgt ccttccatcc cagaacctgt ctggcatcct ctaccggtaa
      481 cgctgccaca gcagcctgca ggtacatgct catcgcttca taagctccga aagtacgatc
      541 caccgcaacg ccgatgcgga acagtgtcgc acagatcttg tctggcagaa gtgcttcaaa
      601 cgggatccga tgaattacgc catcatattg tatatccaga aagtgccggt gtttttcatc
      661 tgccgcaaca cagagaagct caaagtggtc tgccagccat tttacctgtg tatggctggc
      721 aaggtctgcg aatcctgctt cggtgtgcag cacggtgaag ctggttgcat ttacaccttt
      781 tccgaccata aactccagtg cgtacttccg cagacgcatc cattcatgaa ggtcgataga
      841 tgcacctccg aaccgctcca actcgaaccc gacgggaatc ggggtaaact cgatcatgat
      901 gttatacgtc ctttctcaaa caaaaggtga agcgataagc tggtatcgcc ccaccccgtt
      961 tgtctatttt acttttttta ctttagcagt tcaatccgct ttacgttact gaagcgagtg
     1021 ccgctcctct tggatgtcac tgtagagatt tccagtcgaa aagtgtgatt cagaagatca
     1081 tctgtatcgg tggtatttat cgcccgaaaa atgttgctcc acgggtgttt tccaagatcc
     1141 tccagtgtta caaatttcag aaagatcgtg tgatccatgc tggatagttg aaacttgagg
     1201 caaacttcac ccttggcata ttcgaagacg gctatcagtt gagcttcata tgtcccgctc
     1261 tcaagtaggt ctgtttcatc atatagtgtc acgttcatga attcatcctc cattcatcaa
     1321 ttaaacttta cctctgcgta aattgagccg tcttccagtt ggttcgtcgt aaaaattccg
     1381 attttgcgtg actttccttc aggaacttct acgccactca tctcaattag tttttcagtt
     1441 aagcagctgt catacacgta tcctccagaa cgaaatgtta tgctgcactt tgaagtaccg
     1501 tcagtgggaa tgactaaaac ggaatcctct gtaaaataca gctcgattgc aattggactg
     1561 ttcagggcat ctctgaacgt tttgcttaag cgaaatctgc gactgttaac agtgttcgta
     1621 atgatgaagc atcccgcaag actcttatct ggtgtatagg cgtgtgcgtt tacggatgct
     1681 agaaattcgt ttaaattctg tttcataata tattcctttc tcccagtaaa gggactcaaa
     1741 aatcatcttc gagagcgttt tgctctctgc attttattat agtgagtcta atcaattctg
     1801 tcaataggtc gtattttctt gctttatagt gctaatcata tatatattca acgtatttac
     1861 gccgttttta gttttctatt tatcaaagag cggattctcg gcttaggaac cctatggctt
     1921 ccagctgctc caaaagtcag ttgtgcgttt tttatttttt caattcatta tctgcttttt
     1981 tcttgtcgaa aatacgttat tttttgtttt gtatttctgg atagcagcat tcttcccagg
     2041 gatgtctatg gacaaaatgc tctcgtaaag tacgcttgat tttttagaaa gaatctgcta
     2101 taatgagtat cggaaaatgc tgtatcatta ctttactatt tttcagagtg tgcttttgag
     2161 ggattgatct caatgggcat atattttgta aaagatcaca gtaaaaatgt tcatttgtgc
     2221 aacttgatat acttttggtt gcattagcat tattctattg tgcagaacgt agaatcttga
     2281 gatgagggga aaaaaatgtc gaattatcag tatccatggg aggaaatctt tcaagactgt
     2341 gcggatttga tcactgtaca gatcagagac ggcaaaaatg ttccggaaac ctttccgcag
     2401 ttgtatcagc tttttgagaa atggtataca gagcgtgaaa agaaagtacc ggaacagcgt
     2461 cacttcattc gcaaaatgcg ggaagagctg ggcgttgaga gcggaaagaa gaagatccag
     2521 ccgtacttgt acgcctttgt cggaaagtat gacaagatgt cactggagct tctggcagag
     2581 ggaacggcac tttccatggt gtccaacgca tcctggctgt ttgttcggct gcgaagcaag
     2641 atcagcagca cgaccgacaa gaaaaacaca cacttttact atctctcccg caagctaaag
     2701 gaaaagtttc cgcaggacat cctctttctc tcctttgacg tggacaccct tgtgatatta
     2761 tgtaaaaatg aggaatcgaa aaatcgggtg cagtcccatt tccattccat cgaagaggag
     2821 cagtctgtat gagcggcaaa aaaagagggc caaaacccag gtatgaggaa gaagacgttc
     2881 tggaagcgat ggctgtgtat ttctatgagc atatggttgt ccggaaggaa gacctttctg
     2941 tctttacaat ggatgccatt tatgatgaca ttcagcacga tgcttcccag tcgatcctgt
     3001 atcgcaatgt agcatttccc aagtacagcc ggatgcggaa aatgctgatc gaccatcttt
     3061 cagcgtatgg attgtatcag gagaaagaac ggctgactgc tgctgtggca agccggatca
     3121 tagaaactta tgagatcgat gcagagaaag aggacatgga cgtgcttctc tccaatcact
     3181 atacgctgac cacgtatggc ggtccggtca tcacctgcat tgcaaagctt cctgcggtgg
     3241 aagcactgga cacgctggcg aggatcgaaa gcaaggaaaa acagaagtcc ctgacctgga
     3301 gcagaatcaa catgctccgc aaactgtgtc aaaaaatcaa aaaaagaaat gccgatgtga
     3361 tcttagcagt gatcccacag tacaacaaag cgatttacct gaatcaaaac ggcaggatcg
     3421 ataagcacat caacaacatc aatccgttat gtgatacgct tcttttcttt gtcaaagaca
     3481 caccggaggg cagagcgttt gtaaaggaac agaaatttct cgttttccca tttttctcac
     3541 cagaggatga actgcagtaa aaaaatgcgg gtaatccttt tatcccggtg cagtaccgca
     3601 cgcctcagcg gattcgcttt tcgcttgtgc tgcggttcgt cgggcagtaa aaaaaaatcc
     3661 aagtcatgcg tatggcttgg atttttttgt tggattactc gtattggctc gcttgtattg
     3721 gttcgttagt actcttccgc caacagcatg gtgcagtgct ccccatcatc gatgatatac
     3781 accttcgctg tgaccggctt ttctgcctcc gcaatgtacg tcatctcaaa ctgcggctgc
     3841 tccgaggtgt gatgtataat ttgcaaaccg ctctctttgc taagccgaaa cacctgcagg
     3901 tagtctttcg gttcgggcat ctgatcgaca gcctgccaca tcagcacctg aagctcgatc
     3961 ggtatttctg catccacgcc tcgtgtcaag tatctgcctt ttgatttttc aaacatattc
     4021 tttgctcctt tgccttggaa tcaaacatct cgtacagttc gcctcgtcgc atggaaatgg
     4081 tttctctcaa ctccactacg ccaaacccca tatccagcac aatgtcaaaa tcatagggtc
     4141 gcacatgaat cgctttcaca aactgctgga tgaacgtgcg gaactcttcg gtatttcgct
     4201 ttaactcttt ccactgggta tgcaggtatg ccacgtcttc atattgaagt ggttccagca
     4261 gctttagttc gtgcagtctg gtttccagct ttgttttctc attttccagc tgttccgctc
     4321 gctggagcaa gtcatctgta aagatgccct tttcgattgc cctggtgata ttggcgagac
     4381 tgtcctgcac ttcattgtat tgtgcagaaa tcgcttcatg gttggcatca aaatcatgat
     4441 tgaatttctg cacctgttga ttcaccttag caacagaacg ctttagattt ttcggtttca
     4501 gcctttcgcc aatcagcaca gcaatgtagg catctaggta gtccttattc agttccttgt
     4561 tgttgcaagc tgtacggtgc gtattgcatc tgtaggtggc aagccgtgtc ttgctcctgc
     4621 cgctgaatcg aagattcccg atcatccgct tgccgcagac atcacaaacc agttttccag
     4681 tcagcaggta aaactccttg ctgtgatagc atcctgtgtt tcgtttattc ttcgccttga
     4741 tccgctgcac ctgtgcaaac agctttttcc cgatgatcgc aggacagcca ccctcaatgc
     4801 ggatatagct gtccttttga gcgtggttat ttcgtttccc gtctgctctt gccgctgctt
     4861 tattgaacac aaaaacgccg gtgtacttct cattgctgag tatctcatat aaactgttct
     4921 tcccgaacat tttgcctcgt tttgtcttgt atccatgggc gttcagatgg tcgatgatcg
     4981 ctgtatagcc ataaccctct gcaaacattt gaaagatgat ttttactgct tgtgcttcct
     5041 gttcattgac gatcaagtgc cggttttcat ccagatcgta tcccagcggg ggacagccgc
     5101 cggtgtgttt gcactgcaaa gcagtctcgt tcatgccttt catgacttcc cgtgccagat
     5161 ttttgctgta gtattccgac atgccctcca ggactgcttc catcataatg ctctccgggg
     5221 aatcgtccat gcgttccagc acactgcaaa gcttgacatg gttcttcttt agcttctttt
     5281 tgtagatagc actgtcgtaa cgatctcttg aaaaacgatc cagcttatgc accagcacga
     5341 tgtcaaacaa tcccttgccg ctgtcagcga tcatctgctg aaactgcggt cggttatctg
     5401 ttgtcgctga tctcgcctca tcggtatagg tggaaaccac ctgccagtga ttttgcttgc
     5461 agaactgatt catggctcgt atctgtgcat caatggattc acttcgttga ttgtcactgg
     5521 aaaaccgggc gtattgtgca acctttggta tactcagcat cttaccacct caacaggcag
     5581 atagatgtct tcaccgcagc tttcatcaat gattctgaca ccatgcagat agctttctgc
     5641 tccgatttcc aacacttcca caaactctag ttcgttcatc ggaacacaag caaattcctc
     5701 tttttcaagg attacaaaac agccaatgtc atccatgttt tctacttggt attcctgcat
     5761 cattgcttct gcttttttct gaatgtgcat ttgcaaagaa ccattttcga tgcggttcat
     5821 ttcctgcaaa ttggttactt ttatcattca atcacctcca tttcataggg ttctaacacc
     5881 cattctcttc tgactgccgg cgtgagcgga tggcaggcat ttcttccgat ctggtacatc
     5941 ttgccggcat gctgctttat caattccagc aggtatttct gccgttctct gacacgcaaa
     6001 gtattctttc caattgtacc ccaggcaatg ataatggcat cactcattgc agcgtactgt
     6061 tcaatcacgt catctgcttc attgtcaatt agttcatcat cgccattgaa acgaagattg
     6121 agcttttcca tgattctgct gtacaggttc acgatattaa cgctgccgaa gccaagcttg
     6181 ctgacgttat tcagaaccag catcgtggtc atatcaaagt tgacggtatc tgctgtgttt
     6241 ggattgatca tgatgaccat tgcggttggt ttctcgctgt cccattcctt ccgcagaagc
     6301 aaacgatgct ctttgtcgtc tgaaaagatt gctttgctct tgatgattgt ggtttcttcc
     6361 aacatcaaat ttctccctcc aattttgttt caaaaaaaga ggctcagcgt cataccaagc
     6421 ctcccacgaa cagaattttt acttactctt cttgacaagt gccagcagct ctacgccgca
     6481 ggcaacccct gccagaaaca tttcaattgc aatcggcatt gatgtacttc acctcctccc
     6541 gctcctgcat ttcgatgg
//
LOCUS       DAWBGR010000148         6513 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3044_length_6513_cov_5.357076, whole genome shotgun sequence.
ACCESSION   DAWBGR010000148 DAWBGR010000000
VERSION     DAWBGR010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6513)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3044_length_6513_cov_5.357076"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1543 a         1638 c         1878 g         1454 t
ORIGIN      
        1 gacgaaaaat ttgacgacgc atctgtcgca caatctttgt gcggtattgc cctatctacg
       61 gaaaatgtcg aaatttccta ttgacaaatc gacgcgaatc ttgtataata aaaacagagc
      121 aatgctctgc aaaatacctt ataccttatc aagagtggtg gaggaacagg tcctgtgaag
      181 cccggcaacc aatcgctgat gcgatacggt gccaattcct gcggtataac cgagagatga
      241 ggggtttgta caaaaatttt ggacacccta tacgggtgtc ttttttttga tacttttccc
      301 aacgacacag aaaggaagaa tttcatggca agaactttat ttacatcaga atctgtgaca
      361 gaaggacatc cggataagat ctgcgaccag atttctgacg cagtgctgga cgcaattctg
      421 gaacaggaca gcaaggctcg cgtagcgtgt gaaaccattg tggaaaccgg catggtgctc
      481 tgcatgggcg aaatttccac cactgctaag gtggacattc agcagatcgc acgggacgtg
      541 atcgtagaca tcggctacga cagagccaag tacggtttcg acggctacac ctgctccgtt
      601 ttgcaggcga tcaacaagca gtcaccggat attgcgatgg gcgttaacga tgctatcgaa
      661 accagaggtg gcgaatctac cgaaacgggt gccggagatc agggcatgat gtttggctat
      721 gcctgcgacg aaacaccgga actgatgccg ctggcaattt cgctgtccca taagctttgc
      781 aagaagctgg cagaactgcg gaagaataag acactgacct atctccgtcc ggacggcaag
      841 gcacaggtga ctgtggaata tgacggggac aagccggttc gtgtggacac cgttgtcatt
      901 tccacccagc atgccgcaga tgtatcgctg gaaaccattc gccgcgatat gatgcactat
      961 gtgattccgg cagtgattcc ggcagaactg ctggacgaaa acgccaagta tttcatcaac
     1021 cccaccggtc gctttgttgt cggcggtccg cagggtgaca gcggtctgac cggacggaag
     1081 atcatcgtag acacctacgg cggcacggct cgtcacggcg gtggtgcatt cagcggtaag
     1141 gatccgacga aggtggaccg ttccggtgcg tatgctgcac ggtatatcgc caagaatatc
     1201 gtggctgccg gtctggcaaa acgctgtgaa gtacagctgg cttatgcgat cggcgtggca
     1261 catccggttt ccgttctggt ggatacattt ggcaccggca cagtttccga ggaagccctt
     1321 tccaaggcag ttctggacgt gttccagctg acaccggacg gcattatccg catgctggat
     1381 ctgcggaagc cgcagtaccg gaagctggct gcttatggtc acatgggtcg tgaagatctg
     1441 ggcgttgcgt gggaacgcaa ggacaaggtc gatgcactga aggctgctgt agcaaagttt
     1501 cagtaagtaa tccgcaaagc attatgcaaa aggcttttct ccccaaacag gagaaaagcc
     1561 ttttttgcaa attttttcga aaaaagtgtg accttttcgc aggaacggtg tctatataag
     1621 tgaaacactg cgtcaagtat tccagacagc ttcacgcctc tgtgtcactt ctcctaagga
     1681 aacactgcat aaagcttgtg cgtaagatgc gtcgtcagat ttttcggcag attgcacaga
     1741 aattccgcag gcatactgta tgtatggcaa gggatttctg ggcaatatga cggaaaatct
     1801 gcaagcagat tacgtgcaaa gccgtcaggc gggctttatg cagtgtttcc ataagcacag
     1861 aggttgatat actcattccc aagacattgc tctgcttctt ctccgaggca gggcattgtt
     1921 gtttcctttt ataagggatc ggcaggcatt ccccgcagca atgcccccag ctgttccacc
     1981 tgctgtgcca tgcgttcagc gttggattcc ggcagaaatg tggtggcata gtcgtaaacc
     2041 gccagcccgt ctgtgccgtc ggtttccagc acctgcgttg cctgccggac aggcaagtcc
     2101 cactgtgtcg tccattccat actgccggaa cctgcccact ggtcaacttt gccgtacttg
     2161 tgagtgccga tgccgatcac cagcgaaacg ctgtcatagg tgacagtctg cacccattct
     2221 gtgacagttt cggaaaaggg agcggcttcg ttttcgaagc cgaagtatac ctgtgggatc
     2281 aaaacgtcac agtagccggc ttcactgccc cagcggcgga catcggcata ttgtttggta
     2341 tagtttccgt ccatggtgcc ctgcggactg atgctcagca gcagcttcgg tgattttgcc
     2401 ttgacagtgc tgtaaatttg ctgtatcatt tcgttgactt gggagagccg gaactggttc
     2461 aggttgtccg ccttggcatc tgcaaaggcg gcggcatcaa aggcggtttc tgtggtgggg
     2521 tagaagtagt cgtccagatg aatcccgtcc acctcgtatt gctgtacgat ttccgccacc
     2581 ccgtcggcga tcagctgccg tacctccgga taagccggat tcagccacca gtaatcgccg
     2641 accttgcaga gataggtgcc gtttttctgg ctgtcgcggt accactgtcc gatctggtaa
     2701 cggtcagaga gggcacccat gccggcatca ttttgcagcc gcaggggatt gatccagccg
     2761 tgaacagaca ggtgcagccg gtgtgcctcc tccagcagaa tgggaagcgg gtcgaaatct
     2821 cctactgctg ccgctggggg aaataactgg gagcagtaat aggcatcacc gaaggcacgg
     2881 agatgcaggt ataccgtgtt gatgcccata tctgccgcct gctggaatcg ctgatgcata
     2941 ctctcccgaa attgttctgc cgtttttccc gcaagagtgt cggcgtagtc cagatagggg
     3001 aaccacatgc cgtaggtagt ctggtagttg cagactgctg cggtgtccgg cgtttttgta
     3061 gggcagggtg tggtgccgct tgccacgtgc agctgctgta cctgctggac atgcttggtc
     3121 aggtcgtcgc aaaaggcggc gttttccggt acgtggtgtt cggactgtac cgtgcagccg
     3181 ccgagggaaa ccagcagaaa aagagtgaga aatcgtgtga tcttgtgtgt catatgtgcg
     3241 taacctccga aaacagcgaa aatatcgcaa aaaaacttgt ggaaatacag cgaatcaccg
     3301 aaaagaaaaa atcgccttgc attgccctat aaaaaatgtt ataataaaag tatgtttctg
     3361 attgcgaaaa aatgcacggc ttgttcggaa cggccggcag agaaaaggag aattttttat
     3421 gttatcggat atcgaaattg cacagaatgc agaaatgtat cccattggca agatcgctgc
     3481 ggagctgtcc attgcagagg aggacattga gtactacgga cactacaagg caaagctgtc
     3541 ggagcagctg ttccagaagc tggacagcaa gccgaacggc aagctgattc tggtgactgc
     3601 cattaacccc acgccggcag gcgagggcaa gacgaccgtc agcgttggtc tggcagacgc
     3661 actgcatcag atcggcaaga aaacagttct ggcactgcgg gaaccgtctt tgggaccggt
     3721 gttcggcatc aagggcggag cagccggcgg cggctatgca caggtagttc ccatggagga
     3781 catcaacctg cacttcaccg gtgatatgca tgccattaca tcggcaaaca acctgctctg
     3841 tgcgatgctg gacaaccata tgcagcaggg caatgtgctg cggatcgacc agcgtcgcgt
     3901 catgctcaag cgtgtgctgg acatgaacga ccgtgctctc cggaacattg tcatcggtct
     3961 gggcggcaag gtagacggca ttcccagaag cgacagcttc cagatcactg tagcttccga
     4021 agtcatggcg attctctgtc tggcttctga tctggcagac ctgaaagaac gtctgggcaa
     4081 gatcctggtg gcatacgatc tggacggcaa tccggtttat gccaaggatc tgggggcaaa
     4141 tggtgccatg acagctttgc tgaaagatgc actgaagccg aatctggtac agactctgga
     4201 acacacgccg gcactgatcc acggcggccc gttcgccaat attgcccacg gctgtaactc
     4261 gatccgtgct accaagctgg cactgaagct gggtgattac tgcgttaccg aagcaggctt
     4321 cggctctgat ctgggtgctg aaaaattctt tgacatcaag tgccgctacg gtggtctgaa
     4381 gccggactgt gtggttctgg tggcaactgt ccgtgccctg aaatacaacg gcggcgttcc
     4441 caagacggaa ctgactaccg agaatctgga cgctctgaaa aagggcattg tcaacctcca
     4501 ggtgcacatt gaaaacatga agaagtacgg cgtgccggta gtggttgcga tcaaccgctt
     4561 ccagtgcgac accgatgcag aactgcactt cattcaggaa ttctgcgaga gtctgggtgc
     4621 agagtttgca ctgaccgaag tattcgccaa gggcggagca ggcggcaagg aactggcaga
     4681 aaaggtctgc aaaacgatcg aaaccaagcc gtccaacttc cacgtattgt atgacaccga
     4741 actgtctgtg gaagaaaagg tggagaagat cgctaaggaa atctaccgtg cagacggcgt
     4801 gacctttact gcaccggcaa agaaagcact ggcacagatc aacgcactgg gctgcggcaa
     4861 tctgccggtc tgcgtggcaa agacacagta ttccctgtcg gacgatgctg ccaagctggg
     4921 caagccggag cattttacca ttaccgtccg tgacgtgaca ctgtccaacg gtgccggctt
     4981 tgtggtagtg ctcaccggca acatcatgac catgccggga ctgccgaagg caccgtctgc
     5041 cctgaagatc gactgcgaca acaacggcaa tatcactggt ttgttctaat cgaaggagat
     5101 gcacagacgt gttgaaaata cagaaaacaa cggcttctcc ggcggaaacc gtcgcctttg
     5161 cggagcaggt gggcaagcgg ctgcatgccg gtgatgtgat cgcctatttc ggcgggctgg
     5221 gtgtgggaaa aactaccttt acccgcgggc tggcgaaagg catggggctg ccggacgtgg
     5281 tgtcttctcc cacatttgcc attgtgcagg agtaccacgg cagcgggctg tcgctctacc
     5341 actttgacat gtaccgcatc gagggggcaa tggatctgga atccacgggc ttctacgact
     5401 atccgctgga cgagggcgtg tttgccattg agtggtcgga gaacatcgtg tcggaactgc
     5461 cggcaggctg tctgcggatc accattgaac ggctttctga tgcggcacgg cagatcacaa
     5521 tagaaggaga tgagcgtttt gcggatcttg gggattgata cttccggaaa agttgccgct
     5581 gcggctctgt atgacagcga agccggctgc ctgctggcac agcagtctgt ttataccaag
     5641 cgtacccatt cgcaggtgat cctgccgctg gcagagcgtc tgttggcaga taccggtttg
     5701 cagtggcagg acgtagacgg ctttgccgtg gcaaagggtc cggggtctta taccggtctg
     5761 cgtatcggca tcgccgccgt gaaagccatg gcgtatgccc tgcaaattcc ctgtgtggga
     5821 atctccacgc tggagggact ggcgtggcag aatctgtcgt ggcagggcgt gatttgcagc
     5881 gtgatgacgg cacggcaggc tctggtctat gccggactgt atcgcagcga cggaactaca
     5941 gttacagctc tccgtccgga cggcatgcag ccggtggcag aactgtcgga acagctggcg
     6001 gctctgggag aaccagtgct gctggtgggc gatgcaatgg aggagattcc cgcttcggcg
     6061 tttctgcatg ccgcttcgcc ggcgacaaaa ctgcaaaacg gtgccggcat ctgtctggca
     6121 gcagcacagg cggcatggca gacaccggag gaactgtttc cggaatattt gcagctggtc
     6181 aaggcggaaa aggacttgca ggaaaagcaa agggcaaagt gactttatgt acacggaaat
     6241 caattttcaa aaatgtgttg atttaaattg tgccaaatta tgaagtttga caagtgaggc
     6301 tcccctgaaa ggggagctgg cagtgcgtag cactgactga ggggtttcca cttatgccgg
     6361 aaacgccata aaaggatttt acgaaaaaac ctctccaccg cctaacggcg gtccccctcg
     6421 tactgagcgg tcccctctgc cctgcgggca tctccccacc ccgtggggag tcaccccttt
     6481 caggggaggc tatcatgaaa attgatttcc gtg
//
LOCUS       DAWBGR010000149         6431 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3087_length_6431_cov_5.034191, whole genome shotgun sequence.
ACCESSION   DAWBGR010000149 DAWBGR010000000
VERSION     DAWBGR010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3087_length_6431_cov_5.034191"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1550 a         1568 c         1841 g         1472 t
ORIGIN      
        1 agcatttttg tgaaatatga cggaaaattt gcaagcagat ggcgtgcaaa gtcgccagac
       61 gctctttgtg tggtattgcc tttatgtatt caacagccgg tgatgcggct gtgaaggaat
      121 aaacaagtac cggaattccg gtgcgaaagg agttatcatc atgattcgac tgattacagg
      181 taaaaaggga agcggtaaga ccaagatcat tatcgataga atcaacacag cagtcaagaa
      241 caccaacggc tgtctggttt gcatcgaaaa gggcgaaaca ctgcgtcgtt ccatcagcta
      301 caaggttaga tggtgcgata cagagcagtt tgcgatcgac accttcgatg cattctatgg
      361 tttcgtagca ggcatgctgg ctggcaacta tgacatcaag gagatctatg tagacggtat
      421 cctgaagatc gctggtgcgg acttcgagga actgggacgc actctggaaa agctggacaa
      481 gctgaccggc gacgattccg ttgtgacttt caccgtttcc gcagacgttt ccgaactgcc
      541 ggcttctgtg accaagttcg tagactaatg ggtctggatt cccgataaca gggaaaaatg
      601 ctgctttttt cgaatttttt gaaaaaggac ttgacaaggc agctggaaat ttgttataat
      661 aaatgatgca atgcgtgaaa ggaacaggta tgattctaca gttacgggag atttttcaga
      721 tagagggcat gcatctgccg gtggattata cgatctcgct ggaagaactg tccgaggtac
      781 gcggctatac atttgccgca ccggtgtctg tacagggcga gttttcgaac cgtgccggta
      841 tcgtgacact gaaatatacc gtttcctgta ccttagatgc ggtctgtgac cgctgtctgg
      901 cggaactgcg gcgggactat tcgtatgact tttctcacac agtcgtaccg tccctccaga
      961 gcgaaggcga catctatgat acatacctcg tcgcacaaca cgacagcatt gatatgaatg
     1021 agacagcaat ctcagacctt ttgctcatgc tgccgacaaa gatgctttgt cgggaggact
     1081 gcaagggttt gtgcgatatt tgcgggtgca atctgaacga aaccacctgc gactgtcgaa
     1141 agtagaagga ggtgccaaaa catggctgta ccgaagaata aggtctccaa ggcaagacgt
     1201 aacaaaagac gttctgctgt ttggaagctc gatgcacctg caatgagcac atgcagcaac
     1261 tgcggtgcgt tcaaagctcc gcacaaggtc tgcaagaact gcggatttta caagggcgtt
     1321 gctgttctga agatggacga tgcagaataa gtgctgactg acaagacgat tttgttttgt
     1381 acgaggagga gaggcggctt gtgtcgcctc tcttttttgt atgctgtcct cggattgggg
     1441 gcatgatagc gggggagttg tcaccgtagg tgactgaggg atacttccat aaatctttta
     1501 tatgaagcaa aagatttttc ctagcaggct cccttgaaaa gggagctggc agccgtaggc
     1561 tgactgaggg attattgcat gaatctttta caccaagcaa aagattccac aaagaacccc
     1621 tccaccgcct agcggcggtc cccctcccct ttcaggggag gctaatggaa ggcatactac
     1681 agtacagcag tacagcgctt ctgactggaa atagaacgaa tcaaaaaatc aaggagatga
     1741 aaaatcatat ggcattacct gttgtagcgg tggtgggacg acccaatgtg ggaaagtcca
     1801 cgctgtttaa taagctgatc ggacagcggt tgtcgatcgt cgaggatacc cccggcgtga
     1861 cccgcgaccg gatctatgcc aagggcgaat ggcggaaccg ttcgttcatg ctggtggata
     1921 ccggcggtat cgaaacggtg accgatgacg tgattctgaa gcagatgcgg cggcaggcgg
     1981 aactggcgat cgaacgggcg gacgtgatcg tgctggtgac tgacgttcgc tgcggcgtga
     2041 cggctgatga cagtgccgtg gcaagtatgc tccagaaaag cggcaagccg gtggttctgg
     2101 ctgtcaacaa gtgcgattct atcggggaac cgccgatgga actgtatgag ttttacaatc
     2161 tgggcatcgg cgacccgttt ccgatctctg cggcacacgg tcacggaacc ggcgatatgc
     2221 tggacgcagt ttacgatctg ctgccgccgg aggatcagct ggaggacgac gaagatgcca
     2281 ttcatgtggc aattatcggc aagcccaatg tgggcaagtc ctcgctggtg aaccgtatcg
     2341 ccggcgaaga acgggcgatc gtgtccaata ttgccggcac cacccgtgat gcgacggata
     2401 cgactgtgga aaacgaggac ggcaagttcg tgctgatcga cactgccggt atccggaaaa
     2461 aagcccgcgt taccgagcga atcgaacact acagcgtgct gcgtgcctat atggcggtag
     2521 accgtgcgga cgtgtgcctg attctgatcg atgccacaga gggcttcacg gaacaggatt
     2581 ccaaggttgc cggctatgcc cacgaacagg gcaaggcttc cattatcgtg gtcaacaagt
     2641 gggatctgat tgaaaaggac aacaccacca tgaaggagtt cgaggaaaaa ttgcagcggg
     2701 atttcagctt catgtcctat gctccgttcg tgttcatttc tgcaaagacg gggcagcgtg
     2761 tgcccaagct gttcgcaaag attcgagaag tctacgagca gaacgctatg cggatctcca
     2821 caggcatgct gaacgatgtg ctggcatatg ctaccacacg ggtgcagccg ccctcggata
     2881 aaggccgccg cctgaagatc tattatatga cgcaggcaag caccaagccg ccgacgtttg
     2941 tcatttttgt caaccgtgca gatctgttcc acttctccta tcagcggtat atcgagaacc
     3001 agatccgttc caccttcgga ctgaccggaa cgccggtgcg gtttgtggtg cgggaacggg
     3061 cgagagatga aaaataacgg gagagaatat catgctgatt cttgcaatac tcatttctgc
     3121 ggtgctgtcc tatctgctgg gcagtttcaa cagttccatt ctggtggtgc ggctgctgaa
     3181 gcatcaggat attcgggagt tcggcagcca taacgccggt ctgaccaaca cgctccgctg
     3241 cttcggcaaa ggatgtgccg cgttgacgct ggtgggcgat ctggcaaagg gcattgttgc
     3301 cgtgctgctg tccaagggca tctgcgaact gctgggtaca gggctgacgg cacagaacga
     3361 tgtgcatttt atcggctata ttgccggtat tttcgcaatt ctgggacacg tgttcccgat
     3421 ctattaccac ttcaaaggcg gaaaaggtgt gctggtgggc gtgtcggtct ttctgggcat
     3481 tgactggaaa gtgttcctct gcctgatcgt cattttcgct gtggtgctgg caatctcgaa
     3541 gtacgtttcc ctcggctcga tcatcgcggc ggcatgctgt ccggtggtga cgtttttgtt
     3601 ccagttctgg cagcggggcg atctgcccat gtggtacctc tggctcaata ccggactggc
     3661 ggcactgatg ggggcgtggg tcatctatat gcaccgcacc aatatccagc ggctcaaagc
     3721 cggcaatgaa aacaagtttt cgttccattc gaaaaaggcg taggggttcc ttgtggaatc
     3781 ttttgcttga tgtgctaggt cttacggaag tacccctcag tcacctgcgg tgacagctca
     3841 gcacaaggca cgcccttcgg gtgccctaaa ggggagccta aaatgaaaga gggagcctga
     3901 atgtaaaaga gcctcccctt taggggaggg gaaccgccgc taggcggtgg aggggttcca
     3961 caatgttcag tagggtgcga tgtccacatc gtcccgtgta ccagtaaaat tcacgaaaaa
     4021 atccaacaca cgcccaaaaa cccgaaattg taaaggaggt tcatatggca aaaatcacga
     4081 ttcttggtgc aggcggcttc ggtgtcagcc ttgccattgc agcatttcag aacgggcata
     4141 ccgttacggt ctgggatatt tcccagcaga tcgtagatgc aattctgcgg gacggagagc
     4201 acaagaccaa gcttccgggg gtaaagatcc cgaaaggcat cggattcacc acagaccctg
     4261 tctgtatgga acacagcgac atggtggttt ttgtggtgcc gtccttgtat atccgttccg
     4321 tggcacagcg tgtgaagccc tatcttgacg aaaatgtcat tctggtcaac gccagcaagg
     4381 gtctggagga aaagacgttt ctcaccatga gccaggtcat tcagtcggaa tatcccgaca
     4441 atgcggtggg cgttatcact ggcccgtccc acgccgaaga agtgggcaga ggtgtgccca
     4501 ccaccgtggt ggctgcgtcc aaaaatgaag ccgctgcggt gcagatccag gaaacgttct
     4561 ccagtcacac cctgcggatc tacatcaata cagatcctgt gggctgcgag atcggcgggg
     4621 cattgaagaa tatcattgca ctggcggcag gcatctgcga cgggctgcac tgcggtgaca
     4681 acaccaaggc ggctctgatg acgcggggca ttcacgagat caccaatctg ggtgtcaaaa
     4741 tgggcggcag agcggaaacc ttcggcgggc tgtccggtat cggtgacctg atcgtcacct
     4801 gttgcagcat gcacagccgc aaccgccgtg ccggaatcct gatcggcgag ggaacttctc
     4861 ccgaagatgc agtcaagcag gtgggcacgg tagagggcta ctactgctgc cacgccgcgt
     4921 gggaactggc acagcagcac ggcgtttcca tgcccattac ggaacagctg taccatatcc
     4981 tgttcgacgg tgctgacccg cagcaggcac tgaacaacct catgaaccgc ccgaaaaaac
     5041 atgagtatga ggagttctga ttttcgctcc ttttttaaaa tgtgttttct ctttagggga
     5101 gggtttgtcg cagacggtgg aggggttctt tgtggaatct tttaattggt gtaaaaggtc
     5161 tatgtaataa tccctcagtc agcctgcggc tgccagctcc cttttcaagg gagcctaaag
     5221 tgaaagcctc ccttgaaaag ggaggggaac cgccgttagg cggtggaggg attttctgtg
     5281 aaatctttta catgatgttt cagattacgc tgaaaaaccg ccgagattac cccacatccg
     5341 ctttccaatc cacattgcag cgaggtgcag cattatgcta gattatcgaa tggacacgtt
     5401 tttgtccctg tgtgaaacca aaagctatac caagacggct gcgatcctcc acctgacaca
     5461 gccctctgtg acccagcata tcaagtatct ggagcacttc taccagtgcc cgctgtttca
     5521 ctacgacggc aaacaggtgg aaatgacgga ggcggggcag tatctgcggg acaaggtgat
     5581 tctccagcgt gcccagaatg tggaaattcg caaacggctc cagcagatgc aggaaccgat
     5641 ctcctttgac atcgggctga ctccgtctgc cacattttcc tttgcactgc cgcggctttg
     5701 tacctatgtc aaccgcaatg cagaaccgcg gatacagttt catgtgagca acacccgccg
     5761 gctgctgcgg ggcatgcaga acggcatgct ggacgcgatc atcatggagg gcgaaattcc
     5821 tgatatgctg gtagaagcga cggaattgtg tcgggaaaac atcattgccg ctgccagtcc
     5881 ggaactttcg gaagaactat acggttgtac atggagcact ctgctgaaac agcccctgat
     5941 cgtgctggaa accggctgcg ggatccggac gctggtacag cggcacctga gccgcagcgg
     6001 catttcgctc tacgattttc gggaagtcat tgaggtggac tcctttattg tgctgaaaga
     6061 actgctccgg caggggctgg ggatcgcctt tgtgctggaa cacaccattc agcaggaacg
     6121 ggacgaaaaa cggctgggga gaatctatgt ggacaccatg cccatgtatg gctctctcta
     6181 ttttgtcacc atgcgggatc gtgccgacaa tgaacagctg tggaaaattc gcgacgtgct
     6241 gctgcaaaag acgttttcgg cgggaaattg atgaaaggtt gttttttttc gagataaccc
     6301 ctcagtcact tgcggtgaca gctcagcaca aggcacgccc tacgggtgcc ctttcagggg
     6361 agcctatata cacaaataag cctcccttga aaagggaggg ggaccgccgc taggcggtgg
     6421 agggattctt t
//
LOCUS       DAWBGR010000150         6398 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3108_length_6398_cov_3.842031, whole genome shotgun sequence.
ACCESSION   DAWBGR010000150 DAWBGR010000000
VERSION     DAWBGR010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3108_length_6398_cov_3.842031"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1452 a         1782 c         1544 g         1620 t
ORIGIN      
        1 tttttgtgaa atatgacggg aaatttgcaa gcagatggcg tgcaaagtcg ccagatgctc
       61 tttgtgcggt attgccatag taattccgca aaataccaca cgaatcagat tcccgccaga
      121 atcagcacag cagccagtat cactgcccgc agcacggcaa accagatctc gtttccgcgg
      181 aagaaccgga agccgctttc tgcgtttttc ttagggcaga tttccagaca cttctgacac
      241 tggaagcagt ccccgtttcc gttccagctg cccttttccg gcagttccag atcggacgga
      301 cagatcttct gacaggcagt acagcccttt gcacagttcg ccttctgccg gcggacggaa
      361 aagaatggca gcaccggcag cattgtaaag acggctccca tcggacacag gaaccggcag
      421 aagaaccgtt cacagaacac catgcccgcc agaatcagca ccagcagcac acagcccagc
      481 cagtaagcac ctaccttgaa gttaccggag cgaagcatag agaacacgtc ccacgggctc
      541 cagccggtga tctcgccata aacgccggta aaacacagca gcacaatggc aagaagcact
      601 gcgtatttca ggaacgacag aggcttcttc cagcttttct tcaggcgaac gggtttcttt
      661 ttgcacttct tacagagaaa cagatacaga gcatgcaccg catctcccag tgtaccgaac
      721 gcacaggcgt agccgcagaa gaatctgccg aacaccacgg tatacgcaca cagtgccagc
      781 agcacagtga gaaacgaact catcaccacg ggagagccag tgccgatctg tgtcagaacg
      841 gacttgatgc cggaaaaggc tgtggtaaat gccgagggca gaaagaggaa gaacaacagc
      901 tgaatggctg cccgcacata ggcatgtacc tttttgggag tgaaaaactt tttattcatg
      961 ctgcaccgcc tttgcctgct ccagtgcatc ttccacagca cccagaatgc ccctggaact
     1021 aaacgttgca ccgctgacgg tatccacctc tgcggtctgt tcgtccagca cctcatcgat
     1081 cacgccctgt gcggcatcga aataggcagt gtcctctttc tcggcagaaa gcacggtaca
     1141 gtctgtgatc tgtccgtgtt cgatcgtgac ttccattttt accggtccgc cgtagccctg
     1201 tgcctcgccg gtataggttc cgtctgcata aggagaagcc ggtgccgcat tctcctccgc
     1261 agcctgagcc tgctgctctg ccagcttttg tcttgcggca gtttcctgct ctttccggtc
     1321 attcaccacg gcattatagg tacataccag cgaaaccgcc agcatgatgt tgatgatctt
     1381 tactgcaaat acctgcacga tttctcacgc ccctttcctt gcctgttcca gtgcattttc
     1441 cacggcacgg acgatcgcat cagaggaaca ggtggcaccg gaaacggcat caatgctgtc
     1501 cgtctgctgc ttttccagaa tctgtgcaat agtgccggca atctttttgg tgccgtcagc
     1561 ggcacgggaa atatagcggt cgttcagctt gtcgtattcc gcaccggagc cattgacatt
     1621 ggaaatgctg acgatctggt cattctcgat ctgcactttc agcgacagca catattcggc
     1681 aaagtcctcg tcagcgtccg gatagcaatc cgcagttccc tcataagtgc cgttttcata
     1741 aatggccgga accggctctg ttgtcgtttc cgtgtttgtc gtaccggtaa ccggtgtgat
     1801 ggtggtttcc ggcggtgccg ttgtcaccgc agtggtcgtg gtcgttgcag gctgtgccgc
     1861 ctgttttgcc gcttccagag cctcctgaat cgcctccaga ataccctccg aactgaacgt
     1921 tgcaccggaa actgcatcca catcagtact ctgccgctgg atcaccagcg agatcagcgt
     1981 tcccgcacgc ttgaaaaacg cttcatcgtc ctcattggaa accacgtgga tcttctgaat
     2041 ggtgccgttt ttcaaggtca gttcaatttt cgtggtaccg ccatatccct ctccgctgcc
     2101 ggtatacaca ccgtccggat agggaaattt gccggtttcc tgcggcactg cgggaactgt
     2161 agttgccggc ggctctgttt tcgctgtggg aactggtgtg gtttctgccg gcacttctcc
     2221 ggttgccgca tttgccagtg cattccgcac cgcttcgatc aagccggcgg aactatacgt
     2281 tgcaccggaa actgcgtcca cattggtgct ctgggaagca atgatatttt gcagcagtgc
     2341 acaggcattt tgcagatagg ggctgtcatc tttggaagaa agcacctgaa tatccgtgat
     2401 cttcccgccg gaaactgtga cctgcactgt catctgtccg ccaaagccgg tacctgtacc
     2461 ggtatacacg ccgtcccgat aggctgccgg ttctgctatg gcttctactg cgggagcatt
     2521 ttcgctggtc ttgttctggc tctcggaagc tgccagaacc ggcagagcac tgtcagaggc
     2581 actgctgccg ccagcctttt gcaacgcgtc ccgcacggca gagatcagcc ctaccgaact
     2641 ataagttgca ccggaaacgg tatccacgtt tgtgctttgc agggcaacga tctgcttgag
     2701 cagtgccgaa gcattttgca gatagggaga atcgtctgtg gagctgacaa tggtaatatc
     2761 cttgatcttt ccgcccttga taaccacctg caccgtgatc ggtccggaaa atccggtgcc
     2821 ggtaccggta taggtgccgt cccgataggt accggattcc gaaaccggtg tgactgccgg
     2881 agccggtgct tttgtcgtta ccgcagcagt ttttcgttcc tttcgctttt ccgttgtcag
     2941 agtgccggtc tgtgttccgg tttccgaacc ggctgcgtcc agcgacagca ccggtgccgt
     3001 ataatccccc agcgaaaccg caatacatac cgccagaacc gctgtaggca aaaagcccag
     3061 aaccggcaga attttcttgg gattctccca ttttttcatc ttgtttcagc ctcctgattt
     3121 gtttcgatta gaagttagtt tactctaatt agtctagcat aaccaaacga aaaaatcaag
     3181 gagaattcta tgtataaact tttgggaatc ctgtgaattc cccgaaaaca gcacaaaaaa
     3241 cggcttgcgt tttcacaaac acaaaccgtt tttcacaaat acagaaccgc acagaatttg
     3301 cattgcaatg cttatgcctt tcctgtatct tcttctgttg cactgttttg ttttttctcc
     3361 agcatggggg tcatgctctt ggcacagatc gccatggtgg aagcgttatg caggaatgcc
     3421 gaagtcgtgg gagcgataat gccgaacaag cccagcagca gcagcgacga attgaatccg
     3481 atgataaacc gatagttccg gtgaattctg gtcatcagct tttcgctcaa tgtccgcagg
     3541 gttgccagtt ccgtcaggtc agcaccacgc agggtgatgt ctgccgcttc tctggcaatg
     3601 tccgaagcat cactcatggc aacagaaaca tctgccgccg ccaatgccgg agcatcgttg
     3661 atgccgtcgc ccaccatgat gacacggtgc ccgtcggctt tcagctgttc cacatagcgg
     3721 tgcttgtcct ccggcagcac ctgtgcaaaa cacttggtga ttcccagctt tgctgcggtc
     3781 acctctgccg ccttctggct gtcgccggtc agcatcacaa cattttcaat gcctgctttt
     3841 ttcagcaggt caattgcttc cccggcttct gcacggggcg gatctgaaat gcacagcacg
     3901 ccggaaagtt tcccgccaat tgccagatag accaccgagc agccgccgga ctgtgcttca
     3961 attgccgcct gctgttctgc tgtgatctcc acatgttcgt cttctgaaac aaaatggtgg
     4021 ctgccgataa tggcacgctt tccgtggagc gtggtggcaa tgccgtgtgc cacgatatac
     4081 tgcacttcgg catgttcttc ctgatgattc agaccgcggt ctgccgcacc cttcacaata
     4141 gcacgggcaa cactgtgagg aaaatgctcc tcgatgcagg ctgcaattcg cagaatctcg
     4201 tcttcgctgt aatcactgaa cgagatcgcc cgttccagaa ctggttcggc attggtcagt
     4261 gtaccggtct tgtcaaatac cacgctgtcc gcttcggcga aagcctccag atatttgccg
     4321 cccttgacgg taatatcatg gtctgcggct tcccgaattg ccgagatcac agagatcggc
     4381 gtggacagct tgatggcaca ggaatagtcc accatcagaa tggacactgc cttggtgata
     4441 ttccgtgtca gcagcagtgt cagaccaaat cccaagaagc tgaacggcac aatgcggtct
     4501 gccagatttt cggcacggct ctgcacgccg gctttcagat tttcggaatt gtcgatcagt
     4561 tcaatgatct tctggatttt ggtattgccg ctaagagcac gtacctgtac acggatactg
     4621 ccctcatcaa caactgttcc ggcaaatacg gtagaaccct ccgccttgcg aacgctcagt
     4681 ggctctccgg tcatggaagc ttcgttgatg ttggcttcgc cggaagtgac agtgccgtct
     4741 accggaatca cactgccggt gcggacacag atcacatcgc ccacttgcag ctctgccatg
     4801 ggaatcagaa cctctgtgcc gtcctgcacc agccacacct tgtccgtctt tacggcaagg
     4861 ctgtccgtca gggctgcctt ggtcttggct ctggtgtagt cctccaacag tgccgagatc
     4921 gacagcatga acatgatcgt tcctgcggtc ttgaaattcc gctgcaacag gcacgcactg
     4981 acagaggcac cgtccagcac atctacattc agatgtccct ccagcagtgt cttgactgca
     5041 cgccacgcat agcggattcc cttgaacaag atcaccgcat ttgcaatggg agccggcagc
     5101 agagcctttt ggagatatcg ctttgccacc agctttacca gatcagtctt aaagttcgaa
     5161 tcgatccgtt ccgaacaggt gtccggtccc agcggtgcca gcagtctggt gtccagtgcc
     5221 gcaactgtac ggatcacctt ctggcgtgct cctgcttcat aatataccag aataccgccg
     5281 ttttctgcat gggcttctac ctttttgaca gcggcagatt ccagcagctg cttttgcacg
     5341 gcagtttcca gttccttgtc gaatgcatac gcaccgcagc ggaaccgaat ccgccccggc
     5401 ttgtcataaa caatctgaaa cttcattatg catcagcttc ttcgcatgcg tctgtgtcgt
     5461 tgatacctgc ctgttccttg gcatcgcagc aaatatcctg tgcttcgtcc ttcatattct
     5521 ggaaggcagc ctttgcatca ttttgcagct tcatgccctt tgccagaccg gaaacagcca
     5581 gctgacgtgt cttctttgca gtgacgatct tcttgccgat caccattgca acagcaccgc
     5641 cgacaacaca ccacagtttt tcattcttaa agcatttcaa cattgtaaag actccttttt
     5701 gatcaatttc agcttgaaaa tgcatttcgc ttctcaaact ctatacctag tatacaccta
     5761 gactttacaa ttgtcaatca ctttgaagga aatattttgt aagaaaaatc agttggtttt
     5821 ttgtgaagat tacatatgta ttatgaattt cagatagttc aagcaaacta actatgtaaa
     5881 aattatagac agctaaccca aaagtcacaa acattctgaa gtgtttcacg aatttttgcg
     5941 gcatgttgta aaaagcatgt aaaaggcatt caaaaatttc agggcagcac cgcacagggc
     6001 ttttacacaa gatgcccttg gtatatttcc agacatgcct gcatatactg tgcatgaggt
     6061 gatctttgtg gcattttcca atttgcagac actgctgcgg caaagcagtt cttccagaca
     6121 gtttctgttg tccctgccgg tggggattca gctgcggctg cacgaacgca gtcaatttat
     6181 ccacacccaa caggaactcc ggcggcatgc ggcatttttg cagcaagtcc aaaggcttta
     6241 ttcagtgttg ccctaagata ttgagcaggc tacaaaaaat ccctgtccgt tgtgcaagcg
     6301 gacagggatt cctttttgaa aaagtttatt agggggtgtt gacactataa tgatatgcga
     6361 attttgtgag gtgatttttc tccgttctag gcaaaaat
//
LOCUS       DAWBGR010000151         6320 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3142_length_6320_cov_5.528172, whole genome shotgun sequence.
ACCESSION   DAWBGR010000151 DAWBGR010000000
VERSION     DAWBGR010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3142_length_6320_cov_5.528172"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1577 a         1687 c         1481 g         1575 t
ORIGIN      
        1 tgccgccgtt ctctcaaatc gcctggtttt tttctgcatt tgcatcgcct ccttgcaaaa
       61 acgcgttcgc cgcattgttt acgtacagta tatcatattt tcgaaaaatt cgcaagcctt
      121 tttacaattg ttttcacagt ttgcatcaaa aaagacgacc gcccgcatgt cacgggcaac
      181 cgtctttgat tggttgtgca attttatgta atgcgaaatc gttacgcctt ggattcttcc
      241 ttttcgtcat tatcgtcaga agcagaatca gcctctaccg aaacggtttc ctttgccggt
      301 tcttctacag caacaggaac cagcttgtcc cgacggtacg ataccatgaa ggcacgcacg
      361 actgcaaata ccagatatgc tacaagcaga agtgcttcta cgataagcat cggacgaatc
      421 atgttggaaa tcgcgttgga tacagaactg gaaacgctgc cgcttgccgg caccagaatg
      481 ttgtacgcat tggtgtacga tgcagtttca aagtaatcgt ctgcggtttc gttgacatca
      541 ttgatcagtt gattcatctt gtcgcagacg ttcttcatgt ctgtttctac tttttcttct
      601 tcctttttgc ttcccagcgg ggtgttctgc aacttgtcca gacgtgtctg ataatcgctg
      661 atctgctgct gcaaagaaga aacactgccc tgtgcatcag tccgctgtgt gatcagatcg
      721 tcatatgcag tagacggctg tgtcagaacg gttccggtat tgtcagcaga accgcctgcc
      781 ataactacca tggaatcctt tttgtagttg ttgatagaat cggtgatcgt gctcagccgt
      841 tccttggcac tgttcaggct gcggttcagg ctgtcaatac gatactgata atatgttgcc
      901 agggaatcct tgtccttggt aacgttcttg cccatgatgt aagaatacag gctggaatag
      961 tccacgctct gcaaagttgc tgttgcttca ctcaggtcag agaaggtata gccggtcttt
     1021 gtagaacgga agcgggtgtt gtcgttggag gacagagaag aaatatactt cttcagagaa
     1081 tccagactgc tggacagtac atccagtgcc tgcgggtaat cataaccggt ataatctaca
     1141 gcagtcaaag catcaccgaa tgcctgatta tatccgtaag tctgcataaa atagatcttg
     1201 tagttgttca gcatggtgtt caggaaatcc gctgcctgag agcgggacat accggaatca
     1261 ccataggaga actgtacctc aaactgtgtc gggaaatagg aaacgtccat gatcttctgt
     1321 gctgcttcaa tagagttcgt gttggaaaaa acactttcat aagcagtcag tttgtcgatg
     1381 gcatcttccg gaaccacacc ggaaacagtg atattattcc ggatcgcatc tgtttttttg
     1441 acatccatgt tcagatccga aagcgtttct tcaatgatcg ccggcgactt cagggaattc
     1501 gcattaaact cgtttccgtt cgggtccagg ccgttttcaa tgccgtcaaa agtgaatccc
     1561 accattgcag tcagcgggct tgccttgctg ctggacacta ccatagaacc gccgaatacc
     1621 aatcctgcca aaatgacagc aaccagcacc cacggcaaaa aatacttttt caactgccgg
     1681 aagatctcaa ctaccggaat ggtaatggtg tcttcttcct gtgtgttcat gcttccaagc
     1741 tgtgccgcaa tttcttccaa ttccggcgtt ttttctctgc tttttttact cattctgtaa
     1801 tactcctaaa tgtaattgga ttggaattgt ttctgcccgt atcattgcac gaccgcagcc
     1861 aattccgaga aaatgatctg caaccgcata ccttcggaaa tacatcagta tgctcttttt
     1921 attataacac attttaaaag ggctgtaaac cggatatttt ttcaaaaaag ttcctgcaaa
     1981 tccttgaaaa aacagcaaat ttcacaagaa atgatacaaa ggcagtcgtt ttaaggaaac
     2041 acggttttga cagaaacgga atttctgtgg tataatatcg gcaaaaggag gccttgtcac
     2101 catgccatat ctgatctatg tgctgcactg cacaggagat accctgtaca ccggcatcac
     2161 cccagacctg cggcggcgaa tgcggcagca ctgcgggcag cttgccggcg gtgccaaata
     2221 caccaggagt catccgccgg aggaactgct gcaagtgtgg agcacagaaa cccgcactgc
     2281 tgctgcacga tttgaatacg ctttcaagca gctgccccgc agccagaagc tgctgcttct
     2341 ggaatcaccg gagcagtggc agaccgttct gccggaactg gcagaggaaa cctacctgcc
     2401 cgcagagcat ctgcctctgc aaagctatct gtcccgatca tgaacggaac gattttttcc
     2461 gctgatattg cagttcttcc ggcaggaaac cacttctcgc caatgcataa gtcacccaga
     2521 ccactgccgg cggcagcagg ttccacactg ccagaaatac tgtctttccc cacgacaggg
     2581 acgaagctgt cacgtctgca ctgcacagat tgcacagctg caaaaacgga gcattcaaca
     2641 gcttgtacca ccggtaccac gtatccggca gcacaccggc tctcgccagc agcagagaga
     2701 tgcccagcag caaaaacggc agcagcaccg agccgctgta aaacagctgg acggcaatcg
     2761 tatttttccg ctttttgtgc tggtcttcgc gggagcggct cagggcatag ttgacacaga
     2821 cacagaccag taccgcaatg gtacagatac agctgatgag ccgaaacaga ccgccgccca
     2881 ctgccgaaag cgttactgcc agaaacagac acaggatctc tgcaaacaaa tcgctgccca
     2941 gagcctttgc aaaatttttg aattccatat tccacctccc atggaaatga ttgatacggc
     3001 atatctctcc cgtcagcggg gatactagac acagggacag aaccgttgct gacagcgttt
     3061 ctgtccattc cccgaaaagg aggttcgacc gatgacttca caggaacaac acgtactgga
     3121 cgaattctac aagaatgccg ttatgggagc cgatgccacc agcaccattc tccccaaggc
     3181 agaccacccc gacctgcggc aggagctctg caaacagcta gaattctacc agtcccgcaa
     3241 agaggagatc cgcagccaga tgcagcaggc tcatgtgcag ccagtccagc aaagcgacat
     3301 ggcaaagttc tgggcaaatg ccaatattca gctgcactgt ctgggcgggg cttctgccaa
     3361 tgagatcgcc aagctgatgc tcaagggcac caacaccggc gtagtacagc tgacggaagt
     3421 gctccacaac agtccggaca tttccgacca gctgaaacgg cagggaaaag cattcgtccg
     3481 ccacgaggag gcgtacatgg aacgcctcaa agcctacttg taatgacgag gtgacaccat
     3541 gaatccggaa caggaaaaag ccagacagag tgccgaggca gagatgaaga tctatacgcc
     3601 tcaccaggtg ctacaggaaa aacgggacaa tatcgctttc gataatgccc cggtccgcat
     3661 ctatgaacga ccctatcacc ccaaccagcc taccgcaccg ttgggataac tccagagcca
     3721 ggggtgcagc ttcgcactgc acccgtacat cacgattttt tcttggcaca cgcggcacag
     3781 agcccgtaca gcacggtttt tgtatggtcg atgacaaagc cgtggtgctc cagtaagtgt
     3841 tcccccagct ggttcaggta atcacagctg acgtgaaaca actgcccgca ctgggtacac
     3901 ttcaagtgaa aatgttccgt gcaggcgtgg ggttctccgg agaggaactg atagcaggca
     3961 ctgctttttc cgtccagcac atattttcgc acaatgccct cttccaccag ctgctccagt
     4021 gtccggtaga ccgtcgccgt tcccaccggt gtttcgttgt cctgcaaatg ccgcaccagt
     4081 tccgccactg tgatatgccg tcccgcactg ttttgcagaa atgccagaat atattcccgc
     4141 tgtttcgttt tatatgccat aatgttgttc gcgtccttcc taaggcaata ccgcacaaag
     4201 agcgtctggc gactttgcac ggtattgccc taacaactta ccagtgaatc caaattgccg
     4261 cagcagatac cgtttccggc atcttgactg cggcaatttc gttgtataca ggcaaaatcg
     4321 tacaagggaa acactgcata aagcccgcct gacggcttta tgcggagctt ccttattcct
     4381 cgatcgtaac ctttttcata acctgcggtt ccagcggctt atcgttgtag tcggtcttga
     4441 cggaagcaat ttcgtctacc acgtccatgc cctctacgac cttgccgaat gctgcatact
     4501 gtccgtccag atgcggagcg tcttcatgca tgatgaagaa ctgggagccg gcagagttgg
     4561 ggttcatggc acgagccatg gagagtacgc cgcgggtgtg cttcaggtcg ttcttgacac
     4621 cgtttgcagc gaattcgccc ttgatgttcc agccggggcc gcccatgccg gtaccgtccg
     4681 gacagccgcc ctgaatcata aatcccggaa tcacgcggtg aaaaatcagt ccgtcataaa
     4741 agccctgctt taccagcttt tcaaagtttt cacaggaaat cggtgcaatt tccggataca
     4801 gttcgatctt aatcttcttg ccgtttgcca tttcaatgac taccatggtt ttttcctcac
     4861 aaaatcagat atttccggag cactggtaca ttgtaactct gtatgctctt tccagcgaca
     4921 gccctgccaa atcatttcgg ctgctgtgca tggtgtgcct ccagatcatt tttattgtac
     4981 cacaaacgca gaaaaattgc aagggcaaca ccgcacaaag cttgtgcgta aggtgcgttg
     5041 tcagattttt cggcagattg cctagaaatt ccgcaggcat actgccgtat ggcaagggat
     5101 ttctgggcaa tatgacggaa aatctgcaag cagattgcgt acaaagccgt caggcgggct
     5161 ttattcagtg tttccttaaa tggaaacata ttctgaatct gcgtttacag cttgcagata
     5221 cgcatcgatc ttttcctttt ttaccttgtc cccgatcacg atcaagacgg actgcccgtc
     5281 cgccaccgga gagatctcga ttttttccgc cgtagcatta actttcagcc agccgccgtt
     5341 ctctgtgggc agagagccct tgatgcggta gatcttcccg cagtcggaat cagcaaaaat
     5401 ctgctgtatc agcggggcaa tctgtttttc cggcatggca acgtgcatga agtagtgcac
     5461 ctcgctctcc agcgtttccg tgcggaactg cttgacatag ctgcaattcc gatagccggc
     5521 ggactgcaat gcggcatagt cagacggctg caaattcgac cagtccatta ccagcaggtc
     5581 cttcggctga aactgccggc tgcactgaat gccggtcaga gcacggttca ggtggttcag
     5641 aatgcgtttt gtcaataccg gcagtgcttc ctcctgtacg ttctgccact tgctgagaag
     5701 cagcttcccg cagcatgccg cctcagaagc cagcagatat tccatttgag cagaaagcac
     5761 ctccggcatt tcggcatcga ttatcgtaag aatgctgccg atctcaaacc actgatccag
     5821 cggcgattca tacagggtat caaaaaattc gtccatatca aaaatgccgg acggttctat
     5881 gatgactcga tcaaagtgct gcatgcccag cgaaatcagc tgagttttga accgccgctt
     5941 gtggcagcac ggatcaccgc cgccggcaat catttccagc tgacagttct cgcacttcaa
     6001 gtcctgtagc atgaccatat ccacattgac tgccccaaaa tcgttttcca gaatggcaat
     6061 tcgctgcccc tgctgaatca ggtgctcggc gtattggtgg atccatgtgg tttttccaga
     6121 gcctaaaatg ccggtaatca gatcaatttt tgtcatgagc gtttttcctc ccttaaagtg
     6181 atgctctcga atgatatacc gcatttatta tagcagatat ggaatcactt tgcaagggaa
     6241 acacggcttc atgccgcaga atcgctaggc aataccgcac aaagattgtg cgacagatgc
     6301 gtcgtcaaat ttttcgtcag
//
LOCUS       DAWBGR010000152         6313 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3150_length_6313_cov_4.705657, whole genome shotgun sequence.
ACCESSION   DAWBGR010000152 DAWBGR010000000
VERSION     DAWBGR010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3150_length_6313_cov_4.705657"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1243 a         1977 c         1721 g         1372 t
ORIGIN      
        1 ctgacgaaaa atttgacgac gcatctgtcg cacaatcttt gtgcggtatt gccgttgcat
       61 tatttctctg caaacaccgt gctttgcagc tgctgcttgg cggcatcgag gtcgatctcc
      121 agcaccgact gcccgccgac atctgccccc tgaaatgtgc cgtccgccgg cacacgctgc
      181 tgctgcatgt cataggtcgc cagcttgaac ggcgtagtaa gagcatagcc gtacagcccc
      241 agagccgaca tgttggtcgt catcttcggc agtgctccca tgcacacagg aagcagccgg
      301 aacgggttgc ccttgacctt gctcatgacc tggctcatca ctgtccgctg ccgttcggta
      361 cgctcaaagt cggcattgcc cacgtagcgg atacggctgt aggagagagc ctgcttgccg
      421 tccagcgtca gcttaccgcc gccgtccagc aggtcgtcct cccgatcgtc gcccatgatt
      481 tcattgactt cgctgatgag gatctcgttg actgcctccg cctcacggtc ggagatttcc
      541 agttccacgc cgcccaccgc atcgatcatg gcggcacacg cggcaaaagt aatcatcaca
      601 taatcgtcaa tatgtacgtc gaaattctcc tccagcgtgt ccatcagcag ttccggcccg
      661 ccataagaat acgccgcgtt cagcttgtcg ctgccgtatc ccggaatatc cacataggca
      721 tcacgcataa aggaagtcat atagatctga tcggtgcggg agttcaggga caccagaatc
      781 atagagtccg aacgcccccg ttcctcgttg gggtcacgag tgtccgtgcc gatcaccagc
      841 acgcttttca cataggcagc gtccatgctg ccgctggtca cgccgcggtc gccggtgctc
      901 acccgattca tgcccagaat gccgatcatg gcaactgccg agtacaggag gaacaccgcc
      961 accacgatcg tcagcagcac ccgaaacagc ctgcccagaa acgagccttt ccggcggcgg
     1021 tgcacagtcg gacggcggct gctctcctgt cggggagccg tttgctgacg gtgagcactc
     1081 tgcggcggtg cactttgtct gcggggcggt tcctgccgcg gcggtgcact gggacgaggc
     1141 tgctgtgccg gtctgggctg gctctgccgc ggggcactct gtccctgtcg gggagcactt
     1201 tgccgcggct gctggcggta gacattctgc tggggctgcc gtgccggctg gcgatacccc
     1261 tgcggctgtc cgccggtgtg cggagcatac ccgccgccct gcggctgccg gtaaccgttc
     1321 tgagagtacc cctgctgccc gtagccattc tgggagtacc cctgctgacc gtagccgttc
     1381 tgggggtatc cctgctgacc gtagccgttc tgggggtatc cctgttgccc gtagccattc
     1441 tgggggtacc cctgttgccc gtagctgttc tgggagtacc cctgctggcc gtagccattc
     1501 tgggagtacc cctgctggcc gtagccattc tgggcttgac ggcgaacctg ctgggcgttc
     1561 aactgttggg tgtcatactg gtcggtcgaa ccgccggatt cagcggcacg gaagaacatg
     1621 gtttcttcgc cgtcggtatt gtcctgatgt ttatgattgc gttccataat aatcaactcc
     1681 ttgcggcggt cagcccgtcg cccagtggaa atggcggcaa cagttgcccg ttgccctccg
     1741 cggtattgcc ttcaatgttt ccttcgctct cacctaccgc agccgcacag ccagggctgt
     1801 gacaacagtg aggatcacgt gatagatcag gaaaaagtat gccgtcatct tctcataggc
     1861 accggtcacc acgtggatca tcgccagcga cagccccagc agtcctgcca ctaccagcag
     1921 gatcacgccg atcactgcgg cacggcgtac ccgtcttgct cccagcagaa gtgcaccggc
     1981 accgaagccg ctgtccccta cggtcatgga agcactgaca cagccaagac caccggtttc
     2041 ccgctggaac aggtggtgca tcgaggtggg gataattttc agataggatt ccggaaaatc
     2101 aaacagagca gagagccgcc gcagcgaaac ggaacagtcc acgctccgca ggatcagtgc
     2161 cacacgctcc tgccgcagtg cctgcatctg ccgtttgaca tgtgggtctg ccgtaatgcg
     2221 aatggagaac agtgccgcca gcatgccgct gactgacaga tacagcacat cgccgttgcc
     2281 ctccgccagt tcggcttctc tggtcttggt gggcagccct tcgatgttgt ggttcgccat
     2341 catttcacgg ctgccgaaca gcacccgctg attggcgatc cagccgcaga agccctgtcc
     2401 gtcctcgcag acgaactcgt ccaccggcag aagcgttccc acatcgggga tcatctccgc
     2461 aaaggcaccc tgtaacatgc tgtcgccgtg gtgcaccaga ctggctgcgt ccagcagcac
     2521 atcgtccacc ttggcaccgg aaaagacctt catgccctcg atctgtaccg agcccttggg
     2581 gaacaggtcg ttcgcctcta ccagcagggc gttggtgtcg aaaaagtcgt ccacgctctg
     2641 atagcccagc atggcactgt tggaagccgc cgccctgcgg gactcccgtt ccagcggcag
     2701 attgaccacc agtgcactgg cagtacagca gcttgccgtc tggagcagtg ccagcatgga
     2761 gcacaggaaa ctgatccacg gcatgccgac cgccgttccc atgcggatca aagtcatgcc
     2821 cacggcgatc agcagggtga caatgccgca gatcggtgtc attgtccggc acagcccgtc
     2881 cgccatatcc gtggaatagg aataccggag aatatcgcac aagccgtccg ctttccgcac
     2941 tgccgtcaca ataggaatat cgtggagcac gccccgtgtg agcagttccg cagtttcctc
     3001 ctgcgaaaca taggagagca cccgcttttg tccctcgcgg gaaatgacgt tcacgttccg
     3061 cagagcacgg cgtaccatca gcagccgccc gatgatattg cagaacagag ccagcagagc
     3121 acagggcaca tagaggtgca ccgtgtccat tgccagaacg tccggcgaga tcaccacgca
     3181 gactgccccc agcaacgccg gcaccagcgg cagcactgcc atgctgtcac tgtccgcatg
     3241 aaaccggagc aggttccaca gcccgttttt cacggtgggg aacatcacgc ccagccccag
     3301 cagtcccagt gccagctgaa tgatgccgta gccgcggggt gtgaccagtg ccgtaaaggc
     3361 accgttcccc agcacttccg tgcacgccag caccgcccct gccacagtca gcaccagcac
     3421 ggcgataaac cggaaaaata ccacattccg gagattttcc agcgttttct tcaccagttc
     3481 caagttgtcg ggctgccgct tttccgcgtc tgccccaaag gtatggctcc gcacccgctg
     3541 gcggacaggg cgaaaccggc tggtctgctg gctgccctga tagtaatagt cctgttcttc
     3601 ctcccagtgc cgccgcgaaa ccggcggttc ttcatccgtt cccaccggct ctgcccgctg
     3661 gaattcgccg gtgacagatt ccttgaggta ggcactgtcc ggcacattcc ggcggcgttt
     3721 ctttttcttt tcccgtgcac gggtggaatc ttccatctgc cgcacattgg ggcggtgccg
     3781 ctggggtgcg gcggtatctt cttccatcag cttgactgca ccgtcatagc cgctgtcgcc
     3841 ggtttcggtg taagaagcct gataccgtcc ggcaccggcg gcgttgacaa aggacacgtt
     3901 ttcccgccgg aggtgctggg tgggaaagcg ttcccgcagc accggtgccg catctgcttc
     3961 ctgtgctgcc tgtgccgcct tgctggcttt gatgtgggag atcgtagact tgatgtccac
     4021 cagttcttcc gcattctccg gcgacggggc aaccgtgtca tagccggcac gttcccgcga
     4081 aagcggacgc tcagacgttg ccgcattcac cagcttttcg gtttccagtg tcccgcggga
     4141 cagaggctgt tccgaggtgt gccggattcc ggagcgtttg ccgtccgcac tgtattcgtt
     4201 taagatttct tctaatgtaa atttatggtc tgccataata cactcctgtg aaaatttgag
     4261 aagtgattct ccgaaaatga ttgttgccgc cgatgcccta aaactgcctg aaacaagccg
     4321 cccctgaagg ggtggagggg ttctcatgtg aaatcttttg cttgatgaaa aagacttacg
     4381 agataacccc tcagtcagtg ctacgcactg ccagctcccc tttcagggga gcctatagct
     4441 accggttcag cacagacacg cccctataga gaagcctgaa tgtaaaaaag ccgccccttt
     4501 aggggagggg aaccagcgta gctggtggag gggttctcat gtgaaatctt ttcctcaatg
     4561 aaaaagactt acgagataac ccctcagact ctccccgtcc gtcactttgc cagccgctgc
     4621 cgcactgcct catacagaaa gatgcccgct gccacggaag cgttcagcga gttgactttt
     4681 ccacgcatgg gcaggctcag catggcatcg cacttttccc caaccagacg gctcatgccg
     4741 aagccctccg agccgatgac aaacgccgtg ccgccggtga agtccgcatc ggtatagctg
     4801 gtgcctgcgg catcggtgcc gtagatccag acattgtttt tcttgaggat ctccatggcg
     4861 gaagccagat tgggcacccg tgccaccggc agccagcttg cggctcccgc agaggtcttg
     4921 cccacggtca gcgtcaggga cgcactccgc cgcttgggaa taattacgcc gtgtgcaccg
     4981 gcagcttctg ccgtccggat aatggcaccc agattgtggg gatcctgaat cccgtcacac
     5041 agcaccagca gcggcggttc gcccttttcg gcggcacgtt ccagcagttc ttccagcgaa
     5101 acgtatgtgc cgcaggcacc ctccgccgca acgccctgat gcactgcccc gccggtcagc
     5161 tgggtcagct tggactctgt cacaaccttt acgggaatgc cctgctcctt ggcaaggctg
     5221 atgatctcct gcaagccgcc gctttcctgc cccacataga gcatgtcgat tctggcaccg
     5281 ctgcggagtg cttccataac gggatttctg ccggcgatcc ggtcattttc cgtttccggc
     5341 atttccgcag gcttctgccg gcggggctgt ggttttctct ggctcatata ttcctccgta
     5401 tctcctgcgg catggcattt ccccacgccc gttgcaggac actgtctgtt tttttacgat
     5461 gctatccatt atagcatatc tccggagaaa atacaaggtt tttcgtgtga ggggggaatt
     5521 ttaacaagaa agattgcaca ggaacccctc agtcacctgc ggtgccagct cccctttcag
     5581 gggagcctat aagctaccga ttcaacacaa ggcacgccct tcgggtgccc tagcctcccc
     5641 tgaaagggga ggggaaccag cgaagctggt ggaggggttc ttccgtggaa tctttttcct
     5701 cacgcatcag gctctacaaa agaatctctc agaaaagctt tttacattat agggacactt
     5761 tcaacaatca ccatccccca ctacagcacc agcaaacaaa ccgccgccgt gcccagacag
     5821 ctcagcagca ccagcaggag cgtccggagc cagaagcgtt tttctgcccg ttccggtgcc
     5881 gcttcccgca gcagtgcgtc cgcgtccgcc tgcgaaagct gctggggcag cttggtcacg
     5941 ccgggcttga gatagaacac cgcacgttca aaatacagac tgtccggacg gttcacttcg
     6001 atgatttttt tctgtacgcc ttttaccata gccattcctc ctgcggcagc cggtgcatgc
     6061 cggtactgcc gaatgatttc acaggaagta tgtgcacatt gtgaaccact caaacagaac
     6121 aaacctgttt ttccacaaag tcccccgttc cgacaggaat ttctgtggaa aactctgtgg
     6181 aaactgtgga aaacgtccgg tttttccaat ctgtccccta ttgtggaatc cggtggagaa
     6241 cgtttgaaca gtgttttatt ttaggcaata ccgcacaaag attgtgcgac agatgcgtcg
     6301 tcaaattttt cgt
//
LOCUS       DAWBGR010000153         6298 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3156_length_6298_cov_4.174275, whole genome shotgun sequence.
ACCESSION   DAWBGR010000153 DAWBGR010000000
VERSION     DAWBGR010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3156_length_6298_cov_4.174275"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1540 a         1498 c         1731 g         1529 t
ORIGIN      
        1 tctgcaagca gattgcgtgc aaagccgtca ggcgggcttt atgcagtgtt tccttcattt
       61 cggtgcgttt catcgttctg caattatcat atcataattg ggggaagaag taaacacaaa
      121 acagttccga aattgcagag ctttttcgga actgttttct cttgacatat gcggctgcga
      181 agccggtttc agggtgtgtt ctgctgtaga gattcctcct catgctgccg ccgcagggcg
      241 tactttcgct tggtggcaag accgccgcgg atatgacgtt cctgtttgtt ttgctccaga
      301 atttccttga cctgtgtata tagatcgggc tggatctgtt ccagtcgttc gctgacgtcc
      361 ttatgtacgg tgcttttgga aacgccgaac cattctgctg cggcacggac agtggctttc
      421 tggcggacga tatactcgcc gagaatcacg gcacgtgctt cgggctgttc tttcatgatg
      481 cctcactcca attgtggttt tgctacagca tatgcagtac cggcggaaat aagaacgaga
      541 gaagtgctgt gtcggctttt cttacagccc acttgcagat tttaagaaaa gtttatgaga
      601 aatcgttgac gtattccggt aattgtgtta caatagagaa cagcggcata aggtgactta
      661 tgcagagtgt aggacactcc ggaaatacag agtgatccct gacggatcat ggaaaggaca
      721 ttggtacata tggtttggga actcatcaag tccctgattt tcggtattgt ggaaggcatt
      781 acagaatggc ttccgatcag cagtaccgga catctgattt tgctggacga atttttgaag
      841 ctggacgcaa cagataattt caaggaaatg tttgacgttg tgattcagct gggagctatt
      901 ctggcggttg tcgtcatcta ctggaaaaag ctgtggcctt tcggcaagca ggataacccg
      961 caccccatgg cacagaccgg cttcggggca tgcctgaaaa aggacattct ccagatgtgg
     1021 tgcaaggtca tcgtggcaac agtgccggcg gcagtgatcg ggcttctgct ggacgattgg
     1081 agtcaggcac acctgtacaa tgcgtggaca gttgtcatta tgctgatcct gtttggtatc
     1141 gcgtttatct ggattgaaaa gtggcataag aaccgcaccc cgaagatgag caacattgcc
     1201 tcgctgcgat acaataccgt tttgatgatc ggcatctttc agctgatcgc ggcgatcttt
     1261 ccgggaactt cccgttccgg tgcgacgatc gtgggagcac tgctgctggg cgtttcccgt
     1321 acagcggcgg cggaattcac cttctttctg gcgattccgg caatgttcgg ggcaagcctg
     1381 ctgaagctgg tgaagtgcgg actgccctct accggcatgg aaacggcgat gctgctgatc
     1441 ggtatgatct ccgcgtttgt ggtttccatt gtggtgattc gtttcctgat gagttacatc
     1501 aagaagcatg acttcactgt gttcggctgg tatcgtatcg ttctcggcgt tctggtgctg
     1561 atcggcggta ttaccggatt tattggttaa gaaaataaag aaaatccccg tgtatccttg
     1621 ttgcgttgga tacacgggga ttttttatgg gctacgtttt tttactgtgt ggtttcggtg
     1681 gactgcgtta cggcagtggt gtaagtggtg ggagccgttg tgctcactgc tgttccgccg
     1741 aggacgatgg catgctgttt cagataattc tcccacagcg acagggagtt gcccagcagg
     1801 tgaatgccgt cagaggtcag ggacgcgtcc agacagccgt tagcgtccat gacagcttcg
     1861 ttgacattca ggtagaagat attgtcctgc ggatttgcca gagcctccag agcctgattg
     1921 cgggcgttga cagtggcgtt gttgattgcc ccgccggggt tatcctgcga agcggcaacc
     1981 accagaatgc tttccagata gatgacagca ttgggctgtg cctgccgtac ctgtgcgatc
     2041 acctgagcat atgcctgtgc ccagctttct gtggtgcctg tgcccagttc gttcagcccc
     2101 agcatgatgt aaaccttgcc gtactgcttt tgtgccagtt tctgtgccag ggtgacactc
     2161 tcgccggtgt tggggttgcc ggcagctttt tccgtgactt tgaaaatggt aagtcctacg
     2221 ctggagaagt agtccgcatt ggtcaggctg ccatagaggg caagtccctc ggtacgggag
     2281 tcgccaatga aaagggcatc gtcaaagtag gaatcgtcta ccgtctgaaa taccggcgtg
     2341 ggagcagttg tagtggtgac aaccggctga ccggcagcca gcgtggtctg tgcggcggaa
     2401 gtggtcgtct gtgaagctgt cgttgcggag ttgtcgattt tcttggcgtt ctgtactacc
     2461 tgccagatgt ttgcaacggc aatggaaacc acgaatgcca agatcagagg gtattgtttg
     2521 agaaatttca ggaaattcat ggaatgctcc tttcaaggca cggtcagaac tggaagtaca
     2581 ggaacgggtt gttggtctgt gtcatcatcg cacgaatgga gagtacaaac agaatcaaca
     2641 gcaacagcag acagaatccg cttttccgga attttttgta gtacatcagc ggcagaggcg
     2701 tactgaacag cacacacggc acgagaattt tccagtagtt gtggaacgcc tgcaccgcat
     2761 ctcgggtgtt ggactgcact tcgtcagagg tgaggaacgg gaacagcctt gtgaaatagg
     2821 tgcccagctg gtgcatatcc ggaatggcga aaaccatcca tgtcagcggt atgatgaaga
     2881 tgatgtaggc atgtttcagc agatgggtgc gttccaggaa tttgccgtag acgttttttt
     2941 ccagtgtcag aagcacgaag aagatcagtc cccacagcac aaagttccag cttgcaccgt
     3001 gccacagtcc ggtaaacgcc cagactgcca gcatgttccg catggttttc cacttgccca
     3061 cacggctgcc gcccagcggg aaatagatat agttcttgaa ccagcgtccc agcgtgatgt
     3121 gccatcttct ccagaactcg ccggcggtgc cggacatgta cgggaggttg aagttctggg
     3181 gaagctggaa gcccagcatt tttcccacgc cgattgccat catggaatag ccggagaagt
     3241 cgaagtagat ctgaaagctg taggcaaacg ctcccagcca cgcatagggg gtggagatac
     3301 tatcataccc gataacggac agattattcc acagaccgcc catggggttc gccagcagcg
     3361 tcttggcact cagtccgagc acaaaggttt ccatgccctc ctccagcaaa cggacatcga
     3421 cccgacggtg tttcagctgc ggctgaattt cactgtacat gcagatcgga ccggagatca
     3481 gctgcgggaa catgcagagg tacacgccca gatccagaat actccttgcg ggacgcgtct
     3541 ttccggtgta gacatcgatc aggtaggacg tgatctgaaa ggtgaagaag ctgatgccaa
     3601 gcggcatcac aagcgatacc ttgggaattg ccgtatgaaa aatagagagc agggaattca
     3661 ggttgccgat caggaagttg gtgtacttga ataccaccaa caatccaaag ttgtaaatca
     3721 atcctgcgat caaatagtgc ttggcggact cagggtgtct ggcatgtgtt ccgctgatca
     3781 tgcgggaaag cccgtagttg atcagcaggg acagcagcag caacagggta taccagccgc
     3841 tgccaatggc gtaaaagaca aggctgccga tcaccagtgt gatatttcgg tatttctgcg
     3901 gtgtggcata atatgccagc aggaaaatcg gcagaaactg aaacaaaaat gcaattgtgc
     3961 tgaaaaccat agatgattcc tcaattcccc ggagagtatt tttcttcaac ggcactgcat
     4021 gtgttttgtg cagtgctgct tttattgtat gcagatttgt acaaataaca acaccccgac
     4081 aagcggggtg tctcatacta tatcataggg gaaaactgtc gatttttcaa gaacttagtc
     4141 ataacaaata aattacaatt cggtgacaaa ctgaattgcg ggatcagtcc ttcttttccg
     4201 gagctgcata gcaggtaaag acctgttcca cgtgcttggg atctttgacc tttgtcaggg
     4261 cactgacgac cggcaccacg atcagagaga gcagcatggc aatggctccg gcgttgatcg
     4321 gcgaagccag attcagcggc aggcttgcgg cagccttgga aagttccgga ttccagccga
     4381 agccgaacag gaacatgtgc accagtgtca gaccgattcc cagaatgaag ctggtccata
     4441 ctgccggctt ggtgatcttc ctgctgaaca gaccgtacat gaacggcccc aggaaagcac
     4501 cggcaagggc accccatgaa taggacatca gtgtggaaat ggaagcgttt ttgttgaacg
     4561 ccagcagtac ggaaatgacc agaaagacgg cgatcagcac acgcatgatc agcatttcct
     4621 ttttgtcaga gcactttttc agattgggtt tgatgaggtc gatcgtcagc gtggaactgg
     4681 aagtcagcac cagcgaagac agggtggaca ttgatgcgga aagcaccagt acgactacca
     4741 gaccgatcag aatatccggc agagccgtgt gcagcaggga cgggatcatg gcatcgaagt
     4801 ccggcttgcc gttggatagg gtagagatca cggtcttgcc gctgccatcc tctgtcagtg
     4861 tgcagaacaa ccggtcaaag ccgcccagca ggtaagaacc gccggcaacg atcagggcaa
     4921 aaacagtaga gatcacagca ccctttttga tgtcgttttc actcttgatg gcgtagaact
     4981 tatgtatcat ttgcggcagt ccccatgtgc cgagagaagt cagtattaca acacccagca
     5041 ggttcagcgg gtcgggaccg aatacggagt tcagggtgtt ggtctttccg ttgacttcaa
     5101 tctctcccag tgcagccaga gccgaagtca gaccgccctg attctgaatg gcacagatca
     5161 caacagcggc gatgccgatg agcataacaa tgccctgaat gaagtcgttg atcgcagttg
     5221 ccgcgtagcc gcccagaatg acatagatcg cggtcagcag tgccatgaca ataatgatga
     5281 ttttataggc accctctgat tcgatctgga acgccatgtt gaacagacgg gacagaccgt
     5341 tgtaaacaga agcggtatag ggaatcagga acacaaatac gatcagtgcg gcggcgatct
     5401 tcagcccttt ggagttccaa cgcttttcga aatattccgg catagtcttg gcacccagcg
     5461 actgtgtcat gatgcgggta cgccgtccca gcacgatcca cgccagcaga ctgcccagca
     5521 gggcgttgcc tatgccgacc catgcggcgg aaacgccgta ggtccagccg aactgtccgg
     5581 catagccgat gaataccacg gcggaaaaat aagatgtgcc gtaggcgaac gctgtgagcc
     5641 atgagccaac actgtggctg cccagcacaa agtcgctgac ggaacgggtc tgccttgcgg
     5701 tatagacgcc gatgcccatc atgatcgcaa catagatgat gatcattcca atggtgattg
     5761 cggttgtcat attcctctcc tcacggcagc gtttggctgc ctgtataaat ttttacaggg
     5821 gcaataccgc acaatctttg tgcggtattg cccaggatgt tttttgcaga tgcaaaaaac
     5881 acacccttcc aattataatc gagttgtcgg tttttgtcaa cccatatttc tcggtttcgg
     5941 gcgatttgct gtatgaatat gaaatatcac aggaatgtta cataatgttc acctggattc
     6001 tggcagaaaa atcggaaaaa ggcttgacaa gccggtaaat gtgtggtatt ctatagttag
     6061 tcaatgctaa ctcttgaaaa agcaagggga aaacacagaa caaagccaat ggaatagcca
     6121 atggaataga ctttatgcga cgtttttcaa agatccccag ctgctttccg gtggttgacg
     6181 aatcatcgga caagcagttc ttgggaaatg ctgcataaag gaaacacagc acgcagattg
     6241 catgcaaagg caataccgca caaagagcgt ctggcgactt tgcacgccat ctgcttgc
//
LOCUS       DAWBGR010000154         6264 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3173_length_6264_cov_3.831857, whole genome shotgun sequence.
ACCESSION   DAWBGR010000154 DAWBGR010000000
VERSION     DAWBGR010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3173_length_6264_cov_3.831857"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1663 a         1470 c         1666 g         1465 t
ORIGIN      
        1 atataattcg cttgacaaat tatttttttg tgctataata agattgtaaa aagaaaccgg
       61 aggtgagcag atgaaacgaa atgcatacag cctgattttg atggacgatg tggttgcggc
      121 agtggatcgc cttgcggcac agcagggcac cagccgttcc aatctgatca atcagatttt
      181 ggcggagcat gtctgctgta ccacaccgga gcagcaaatg cggcaagtgt ttacctgttt
      241 gacacaggcg atgaactcgg cgttccgcgt acaggagcag ggttccgatg ccatgctttc
      301 gattctgggt tcggtacagt acaagtaccg tcctaccatt cgctatagcg tggaactgca
      361 tcgggaactg cacagtgaaa aagtcgggct gctgaaaatt tcctgccgga cccagagccg
      421 gacactgctg gaagcaatac agcagttctt cctgttctgg gtacagctgg aacaggagta
      481 tgaaccggag ggtgcctgtg cactgggttt gtaccagatc gaaccgaacc gcctgacacg
      541 ggtgcttctg cggcatggca ttacgtccaa tgaggtactg ggaacagcaa ccggaaagta
      601 tatccagatg ttccacacgg tgctgcaatc ctattttcag ggcttgcagc agggacttcc
      661 ggcggcagtt ttgcagcatg cattggaaca gcaatatgcc gcactgcatg agcagctggt
      721 cagccagctt taggaaaaca tgaggcagca ccgcataaag cccgcctgac ggctttgtac
      781 gcaatctgct tgcagatttt ccgtcatatt gccctaagca atctgccgaa aaatctgacg
      841 acacatctta cgcacaagct ttgtgcggtg ttgccataag acaaaaaaga gcaacaaaaa
      901 cagaatgcaa gaaataggag ggagttctca tgaacacaca gaaattgaca caaaagtctt
      961 tggaagcgat tcaggctgca aatggtcttg cagtggagaa ccagaaccag cagatcgaac
     1021 aggtacacct gttgagtgct ttgctggagc aggagggcgg tctgattccg cagctgtttg
     1081 agaaaatggg cgtgtctgtc gataacgtac agattcagct gaaaaacgcc attgataatc
     1141 tgccggcagt aaccggaagc ggtcggaagg cagacgaggt ctatgtatct caggacgtag
     1201 accgtgcatt gcgggaggca gaaaaagaag ctgcccgcat gaaagatgat ttcgtatcgg
     1261 tagaacatct ggtactgggc ttgttcgatg ccatgggcaa agaactgcaa aacgtgctga
     1321 aacctttctc cgtgactaag gatgcattcc ttgcggcatt gatgaaagtt cgcggcaacc
     1381 agcgtgttac cgatcagaat ccggaagaaa cttacgatgt gctggcaaaa tacgggcagg
     1441 atctggtaga acgtgcccgt gccggaaaac tggatccggt cattggcaga gataacgaga
     1501 ttcgaaacgt cattcgaatc ctttccagaa agacgaaaaa caatccggtg ctgatcggtg
     1561 aaccgggtgt cggtaaaacg gcaattgccg agggtctggc actgcgaatc gttcgcggcg
     1621 atgtgccggc aaatctgaaa gaccggaaaa tcttttcgct ggatctgggt gccctgatcg
     1681 caggtgccaa gtaccgcggt gaattcgagg aacgactgaa agccgttctg aatgaagtca
     1741 agaaaagcga aggcggcatt atcctgttta tcgatgaact gcataccatt gttggtgccg
     1801 gtaaatcgga cggtgctatg gacgcaggca acctgctgaa gccgatgctg gcacggggtg
     1861 aactgcactg cattggtgct acaacgctga acgagtaccg gcagtatatt gaaaaagatg
     1921 ctgctttgga acgtcgtttc cagccggttc tggtagacga gcctactgta gaagacacga
     1981 tctccattct gcgtggtctg aaagaacgct atgaagtatt tcatggcgtt aagattcagg
     2041 atcaggctct gattgcagcg gctacactgt ccaaccgata tatcacagac cggttcctgc
     2101 cggataaggc aatcgacttg gtagacgaag cttgtgcaat gattcggacg gaaatggact
     2161 ccatgcctac agagctggac gaaattcgcc ggaagatcat gcagcatgag atcgaagaag
     2221 ctgccctcaa gaaagaggac gacaaactgt cgcaggcaca tctgcaagaa gtacagcaag
     2281 aactctctca gatgcgtgac cagttcaatg ccatgaaggc aaaatgggaa aacgaaaaag
     2341 atgccatttc caaagtgcag aaactgcggg aagaactgga gcaggtcaac gccgagatcg
     2401 aacgtgctga acgggactac gatctgaaca aggctgccga actgaaatat ggtcgtctgc
     2461 cgcagctgaa aaaacagctg gaagacgaag aagccattgc agaaaagcag aaagagagca
     2521 agaactctct gctgcgggac aaggtgaccg aagaagaaat cgcccgcatt gtcggcagat
     2581 ggactggcat tccggtaacg aagttgatgg agggtgaacg ggaaaaactc ctgcacatgg
     2641 acgaagtact gcaccgccgt gtaattggtc aggacgaagc tgtggaaaag gtcagcgaag
     2701 ccattctgcg ttctcgtgca ggcattcagg atccgaaccg tccgttgggc tcgttcctgt
     2761 tccttggacc gactggtgtc ggcaaaaccg aattggcaaa ggcactggca gaagctttgt
     2821 tcgatgatga gaacaacatt gttcgtatcg atatgagcga atatatggag aaattcagcg
     2881 tgagccgtct gatcggagct cctccgggat acgtgggtta tgaagaaggc ggtcagctga
     2941 cagaagctgt tcgccggaag ccgtattctg ttgttctgct ggacgaagtg gaaaaggcac
     3001 atccggacgt gtttaatatc ctgctgcaag tgctggacga cggcagaatc accgactcgc
     3061 agggcagaac ggtggacttt aagaacacca ttatcattct gacctcgaac ttaggttcgc
     3121 cgatgattct ggaaggcatg gacgaaaacg gcaacatctc ggaagaagcc agaaaggaag
     3181 tcgatacgct cctgaagcag aagttccgtc cggaattcct gaaccgtctg gacgagatcg
     3241 tcttctacaa gccgttgagc aagaccgaga tcttccgtat tgtagatctg ctgctggaga
     3301 agctgcggaa gcgtctggaa gacaagcagc tggaactgga agttactgat gctgccaagg
     3361 agtgcattgt ggcacagggc tatgatgtca actatggtgc acgtccgctg aaacgactga
     3421 tccagtccaa ggtagaaact cttgtggcaa gaaagatcat tgccgacgat ccggcaccgg
     3481 gtactgtaat tcgtatcgac tatgacggtc agaagatgat ctgtcagtaa aacaatagac
     3541 aattgcaaac ctccaaaata aaacgccacg ccgcacagat catgttacgt ctgtgcggcg
     3601 tggcgttttt tattttgttg aagttgcctt aagcaaccgg aatgctctgt acatcttccg
     3661 gcagcagtgt cagcagttct tcgtccgaaa gcggctccgg cagttttgcc agccggtctg
     3721 cctgattggt cagggcaaat tcgaaaaagt tccggacatc tctggcattg gcaaatcctt
     3781 ttcccgcttc tgcacagcgt ttccggaaat attcttgtac ataggcatag gtgtctgtcg
     3841 gcattttcag acagtagctg tcgcataggt tagagaagat gagcatcagt tcttccggct
     3901 tatagtcatc gaaaaagatc acctgccgga agcgggaacg cagccccgga ttggtgtcca
     3961 gaaactgttc catttccttc ggatagccgg caacgataac gatcaaatcg tcccgatgat
     4021 cttccatttc cttgagcaga gtgttgactg cctctgtgcc gaagtcgttg ctgccgccgg
     4081 aactggtcag cgtgtacgcc tcgtcaatga acaacacgcc gcccatagcc ttttccacga
     4141 ccttcttggt tttgattgct gtctgtccca catagccgct gaccagcgtc gcacgatcag
     4201 cttctatcag atgccctttt ttcaggacac ccagtcgggc atagatttta gacagcagcc
     4261 gtgcaactgt ggttttgccg gttccgggat tgccggaaaa gaccatgtgc agggaagtct
     4321 gcggcagggg cagccctcgc tcctgccgga tcgttcgaat cctgagcagg tgaatcagac
     4381 tttccaactc gtctttgacg ttttgcagcc ctaccagctg gtgcagctgt gccagcagtt
     4441 cgtccagcgt ttccgtagga gcttctgtaa tttcagcatc atcgacgacc gcttcttttt
     4501 cagccggaat cggcggcttt tcggcattga aattcgccgg aatgacagcc gccttttcca
     4561 gttccggacg acgacagcca cgcatggcgg catctcgata acgggaaagc tgctgcatca
     4621 tggagttctg cacgatctgc tgctgtggct ctacttttcc ggcagtattc agcgaaaact
     4681 gcgttcccat ttgtgcaatg gtttccagcc agagaaggga aatgctgccc tctgcaccgc
     4741 tgccctgcaa atcgacacgc accagtgccg ccagcagtgc cagtaaagaa tctgccgcac
     4801 cggaaaagga aatggagaac tgctgcaaaa agcgttccat tgtctgtgtc gtggcagact
     4861 gttgcagaca gttgttgata aaattggttt ctgcatcggt gatgacacgg tcctgatttg
     4921 ccagaaacag cagatatttc attgcggcaa agttggcgta ttgtgtcagc ggcatgcggc
     4981 tgccggtgat cgtgatgccc tgtgcttcat atgtagcgta cagcgtatgc aagtacgcca
     5041 gtgcgtcctg tagatgattg tgtggcataa tgattattcc tttccgtatt tgttgtctgc
     5101 ggtttcatcg gaaagctgat gcttttgtgc agcacgcttg tccacttttc gcttgataaa
     5161 gattcccgct gccgccagca gaacgatcac gcctgccagc acgatcgtca ggatatagtt
     5221 gccctcccat aaactttttc ccataaaaac agtcaggact gttgcaggtg tccgtcccag
     5281 tgttgtcagg aaaaacagat acatcggctt gatttttgga ttgaatgaaa ccagataggt
     5341 aagggcatct ttcggcagcc ccggcaacag aaacaccaga aagatcagca gttctgttcg
     5401 cttgccgtcc agcagaaaat caaatttttt gaaatggctt tccgaaacaa aacggtctac
     5461 cagcggtctg ccgatccact gcaccagata gtacaccaga accgttccca gaaaaaatcc
     5521 ggcaagggac agaaacagac cgccccactg tccgaacagg attccaccga taataggcac
     5581 cggaccgccg gggatcacgg caatgaccac ctgtaacact tgcagcagca caaacagcag
     5641 gggagcgaag atgccgaaac tctgcatttt gtcgatgatg acctgctgtc cgtgctcgct
     5701 ggacagcaaa tggatcagtg gcagcatggc aacgctgacc acagcaaaga ccactaccac
     5761 agcgatcaga ctaaaaatgg ttttccggga aatttttttg tcagatgttt ttttggacgt
     5821 tttatttttc atgtgaaata ctcctttcac agaaatccct ataaaagcga tattgcttta
     5881 gaaatagtat accgcgattt tgtaaaaaaa tcaagggtaa tgctacagaa agcttgtaca
     5941 gaagatagat tatcagattt ttcagcagat tgcatagaaa tttcgccggt atttttgcgt
     6001 atggaaaggc actcctgtgc agtttgacga ggaatctgca aacaggtaaa tttttttgtg
     6061 gtattgccca agattttttg ggagcctatt tatgacattt ttacaaaagg gcttgacttt
     6121 ttcaaagaat gcgtgtataa taaaacgtat catctggtaa gaagccgtgc atagcggcac
     6181 gctgccggtg agtatcactt caggtagaaa ggaacttgtt ctatgaaaac aaatcgagca
     6241 agatggattg cctgtttgtt ggct
//
LOCUS       DAWBGR010000155         6188 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3211_length_6188_cov_3.738464, whole genome shotgun sequence.
ACCESSION   DAWBGR010000155 DAWBGR010000000
VERSION     DAWBGR010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3211_length_6188_cov_3.738464"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1549 a         1452 c         1598 g         1589 t
ORIGIN      
        1 atctgacgaa aaatttgacg acgcatctgt cgcacaatct ttgtgtggta ttgccctgaa
       61 acatgcaaat tctaaaaatg cgtaccgatt tttggtcggt acgcattttt tgtaatatct
      121 taccacatgg tctggactgc cgttattcga tcagtccgct gttcttcagc aggatttcaa
      181 tatccgtgcc cttgactttt ttcagaacga tcttgagcag cttcttttcc acaaaggaaa
      241 gttcttcgtc ggtgagtggc tttttgtcaa tggcataata gcaggcacgc agcagttccc
      301 gcacgtcgta cttgctgccc cagacttccg gcacaccgcg gtcaatgtcc agcagggtgt
      361 agacagattc cataccggta cgcatggaat attccgtggt gaaaatggtg tcacgaggtg
      421 tttctgcaaa ctgcccgata aaggcgaagt tgacggctcc ctccggaacc acacgcggac
      481 ggtcgatttc cttgcggggc tggaaaaagg cgttgatata cggcatgaaa caggtcgtgg
      541 tgttgcaggc gtttttcgcc agcgatgcaa ttttttcttc cggtatgccg atatgataca
      601 gccattcctg acaaacttct tcgccggtgc agtcccgcat tgcctttttg acatagtttc
      661 cctctttatt ggtgttcagg gaatacagcc atatcagcac catgtttttg tcctgcgagt
      721 ggaactgggg ctgccggttg atcgtccacg agaggtacca gttgtcggtg ctgtccttta
      781 cggtgacgat accgccggtg gtgactttgc cggcacgggg atcccgtttg caaacgttga
      841 taatatgttg aatgatctcc tcgtctgcgg tggcaacggt ggcactcatc cagtttgtcg
      901 catcaatgtc gctgcaaaag acttccggat tgccgaattc gccgtgaact gcttgagccg
      961 caatcgcttt ccacagatcc cacgattctc cgcagccgtt tttcagatgg gacagatccg
     1021 gtgcgtgggt ctggtcgccg tagcaggagg tgtctgtgca gcagccgttg gtgatgaaca
     1081 ccagatcgtc ttcgatcagg tcaatggtct gttctttgcc gtctttccgg tatacgatct
     1141 gcttggcaac ctttttgctg cctacggttt caatgatgac gttcttcacg tccatgccgt
     1201 attcgatctg tacgccatgg gcttccagat atttcaccag cggcaggatc atgctctcat
     1261 actggttgta tttggtgaac cgcagtgcac tgaaatccgg cagcccgtca atgtggtgca
     1321 catagcggca cagataccgc ttcatctcca acgcactgga ccaacgctga aaggcgaaca
     1381 tggtctgcca gtacagccag aagttggtgt cccagaatgt gtccggcaga acatctgaga
     1441 tcttcttgtc ctccagatcc ttttccggtg tcaggaatag cttggaaagt gccagagccg
     1501 atgccttgtc cagagcgaat ttcttgtcgg tgtgagcatc tttgccgcgg tcaacagaag
     1561 cacggcagag ggaataattg gggtcgtgct tattgagcca gtagtactcg tccagcacgg
     1621 aaacgtccgg cgtttcgaga gaggggacat cgcggaatgt atcccacatg acttcaaagt
     1681 ggttgtccat ttcccgtccg ccacgcatga aaaagccctt ggtgatgtcc ttacgtccgt
     1741 cgcagctgcc gccggcaagt tccagttttt cgaacaggtg gatatgttcg cccttcatct
     1801 gtccgtcccg taccagatag aatgctgcgg taagaccggc aagaccggtg ccgatgatat
     1861 acgcggattt tttgtccaca tttttcggct tttcgggatg tgcgaatgct tcataagttc
     1921 ctgcggaata atacataaca atgcctcctt ataaaataaa aatagttata aaaataattt
     1981 tttgcggtat cgtccatgca ttttgtttat tggtataatt ctattatacc cgattttcag
     2041 gaattcacaa tcagcaaaaa aggctccgtg ataaaaaact tatcacagag ccgaaaagtg
     2101 taaaaaattc tatcaaaaat gcgaaaatga taaaaacagg gtaataccgc acaaagtcgc
     2161 cagacgctct ttgtgcggta ttgactcagg gttttatgca gtgttccctt tataaccgga
     2221 accgcgaaat ggctgcggtg acggagcctt gcagcagctt tcccagccgg ctgacgatct
     2281 cctgcggatt ttcccgcata tcgttcttga tccagtccag catcacgcca atgaaaatat
     2341 aggcgtagac cttggcgata aactgcttgt cctcgtcccg caaagtcatg cctgcggctt
     2401 cctcgtcgat aacgtccagc agcagttggt ctaccagcgg ctgtaggtat ttctccacct
     2461 gttcccgatg cacacagcgg tagacgttca ggataaacgg cttgttttcc tgtactgctt
     2521 cgaagatttg caaaagtccc tgctgccacg tgtcataggt ctttttttcg tccagagccc
     2581 gtctggcatc ttccagacaa gcccattcca caaggtcgta gatgtccttg aaatggtagt
     2641 aaaaggtcat gcggttgatg ccgcagtcct cggtaatatc gccgatagtg atttttgtca
     2701 gcggcttttt cagggattgt tccagagccc gtttggttac atttgacatc gatgttgtcc
     2761 tttcagtttt ggtaacagat ctgttctccg gtctgcatgg gatagcgaca taactggtcg
     2821 ctgtccagat tttcccgatg catgcagact gcggagatct ggtgattttc ataggtggta
     2881 tagtagtaga tgcccttgtc ggcattgcag caggaagtgt aaatggtgat ctcgtactgt
     2941 ccgtctttca cttcgcagca gccgcgtggc tggtcaacgg ctccgagaat atggaaaaac
     3001 tggctgacgc tttccgcttc agaactgccg gaaacggcat tcgcccgcac gaaagctgct
     3061 cgtacaaagc gggactggga ggagaggtca ccgggaagcc ccagtgctcc catgcctctg
     3121 ctgtacgtct gcaacggcag cttgtccgaa aagttgttct gcggctgctt gggagagagg
     3181 tgcagaaagt cgttgaggcg gaacatctgc ttcggaaaag gcggattgtt ggtcaggaca
     3241 ccggcgggat tgtcatagac ctgaattccc gcggcagtgg cttctactgt aatggcttgg
     3301 tgcttgtcag cgatgagcca gtgcagctgt gccgcaggca tcttttcgtg aaacggcgtg
     3361 cccagcaggc aaagcgtttc cactttttcc ttggcttcgg cagtgctgga gcactggcta
     3421 agaatccacg ggatcaattc atactgtgcc acattgtccg aatgatctgc aggtcgggag
     3481 gcgtagcagg cgttgcccac aaagttcagc ccagccatgc aaagcccctt ttcgttgacg
     3541 gcatcataaa aaagcgggta gccctgggca acgtgtgcag tgccgatcat ggcgtagtgc
     3601 tgtgcagata cgcccatgtg cagaaatgac agccggaact gccgcggcac aatgacgatt
     3661 tcttccccgt aggaactttc gtagtccagc gttctgccaa aataaaaatc atttgtctga
     3721 taagttgcag cggtacacat tggaaattcc tcctgtgcaa taaatttgat atgtatatta
     3781 tagcacaaaa ttcggaaaga tgcaaataat aatattgaca tatgcggaat ttcatgttat
     3841 aatatatcta tatttttgta aaggagaatt tatatgccaa attatgttgt tttacaggcc
     3901 gttctgaagg agaagctgat cggaaccggt tccggaaatc tgtctgaact ggaaaaggtc
     3961 atcaacgcac aggctgccaa gggataccgt ctgcacacga tcaccacttc ttcttccgga
     4021 agcaagggat ttctgggcgg tgaccgcatt caggcaacca tggtgtttga aagcctgaac
     4081 agagcatgaa acgggttctg gtaaagtctg tatatgatgc ctttgaatat gttatgcagc
     4141 atttctactg tttcggacag gcggatatgg cggaacgaaa ggatacgtat gcggtcattt
     4201 cgattcagga tacgcacacg cagggattcg gatttcagtt ttgcgagaac cagttctgca
     4261 agggtgttct gacgctgtac tttgatgaca ttatccgaga ggtggacggg gcagttttgt
     4321 ttaccgatga aatggcagat cagatcattg catttgttct ggcacatcgg tctgtggaaa
     4381 cgctgctggt gcactgctac ggcggacaat cccgttccag agccgtggga gcctttgctg
     4441 tggaaatgct gggcggcaat tccgaaaggt attttgaaac cggttctccc aatcagtatg
     4501 tctacaatgt gctggaaacg gcgtggatca gacggcagct gggggcgttt tgaaacgaat
     4561 tttcatgaga atgcgggaaa cttcttgaaa atgtaaaaaa atagtggtat actatatagg
     4621 tgacacagta cagaaatgag gaggattctg atccatgaat atcgcagttg caggaaccgg
     4681 ctatgtggga ttatccattg ctacactgct tgcccagcac aacaaagtta cggcggtaga
     4741 catcataccg gaaaaggtcg aactgatccg ccagaaaaag tccccgattc aggacgattg
     4801 cattgagaaa taccttgcag aaaaggaact ggatctgacg gcaacgctgg acgcagaagc
     4861 ggcatatcga gatgcagatt ttgtggtcat tgctgccccg accaactatg acagcaagaa
     4921 aaattttttt gacaccagtg ctgtagaagc ggtcattgcc cttgtcatgc agtataatcc
     4981 gaatgccatt atggtcatca agtccaccat tccggtaggt tataccaaga gcattcggga
     5041 aaagaccggc agcaggaaca tcatcttcag tccggaattt ctgcgggagt ccaaggcgtt
     5101 gtatgacaac ctctatccgt ccagaatcat cgtgggaacg gacatggacg atgcaagact
     5161 ggtagaagcg gctcacactt ttgccggact gcttcagcag ggggcaatca aggagaacat
     5221 cgacaccctg ttcatgggat tcaccgaagc agaagcagtc aagctgttcg ccaacactta
     5281 tcttgccctc cgcgtgtcct attttaacga actggacacc tatgcggaac tgaagggact
     5341 ggacacgcgg cagatcattg acggcgtatg cttggatccg cggatcggaa cgcattataa
     5401 caatcccagc ttcggctacg gcggctactg cctgcccaag gataccaaac agctgctcgc
     5461 caactatacc gatgtgccgc agaatatgat gtcggcaatc gtagaatcca accggacacg
     5521 aaaagatttt attgctgacc gcgtgctgga aaaggcaggg tactatgatt acagccagaa
     5581 caatgactgg aatgccggca cggaaaagca ggttaccatt ggcgtgtacc gtctgactat
     5641 gaaatccaac agtgacaatt tccggcagtc ctcgattcag ggcgtgatga agcgtgtcaa
     5701 ggcaaaggat gctacagtca ttatctacga gccgacattg caggacggag caacgttctt
     5761 cggcagcgtt gtggtcaacg acctggaaaa attcaaggcg gcggcagatg cgatcattgc
     5821 caaccggtat gatgtctgtc tggacgatgt ggagaacaaa gtctatacca gagatatttt
     5881 ccgcagagat taaggaaacg ctgcataaat ccgtcaggcg ggctttatgc agtgtttcct
     5941 taacgacgaa cggggaaaaa tcaccccaaa agacacgtgt caggcttcgt gtcaacacac
     6001 ccgaaaacaa aatcgagaaa cagaatggct tggattctcc ggaatttgag ccatttcgat
     6061 ttttttccaa aaaatttgaa aaaaggcttg acaagtcgat caggaagtga tataataaaa
     6121 gcaatctcaa attcgaggaa ggaatgtgag agaaggaata tttatttaaa agtagaatga
     6181 cgtgatgg
//
LOCUS       DAWBGR010000156         6182 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3215_length_6182_cov_3.926718, whole genome shotgun sequence.
ACCESSION   DAWBGR010000156 DAWBGR010000000
VERSION     DAWBGR010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3215_length_6182_cov_3.926718"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1572 a         1708 c         1527 g         1375 t
ORIGIN      
        1 ttgcctcagg tgtataccaa tctgccgaca ggctttcgaa cacaaaacac agagaggagc
       61 aaatctcatg gaaaaacaca ctttcggaaa tgaatacggc tttcagacca gagctgttca
      121 tacaggaaac gacatcgatg cggaaaccgg agcaatcaag cgtccgatca ccatggcaaa
      181 cagctatgcc ctgccctatg accccagtga tctgaactgg agcagtgcag gcaaaaatct
      241 gtacacccga aacggcggct ctaaccagca gtatttacag gaaaagctgg cttcgctgga
      301 gggtggtgag gactgcgttg tgctggcaag cggcgtggca gcactgtccg gcttgttctt
      361 cgccctgctg gaaagcggcg atcatgtgat attttccagt gtgacctata ttgccgtata
      421 ccgcctgctg aacgaactgc tgaaaaacaa gttccacgtg gacgcgacca ttgtggacac
      481 ctctgatctg gaagctgtga aaaaggcgat tcgtcccaac accaagctgg tacacatcga
      541 aacgccgggc aatcccacgc tgacgatctc ggacattcgt gccattgcag acattgccca
      601 cgaaaacggt gctctgctgt cggtggacaa cacctttgct tcgccctata accagcgacc
      661 tattgaactg ggagccgatt tcaccgtgga aagcctgacg aaatatatca acgggcacgg
      721 agatgccatg ggcggtgcga tcattggcaa aaagaaatat ctggacatca tccggacaca
      781 atcgcaggtg aatctgggtg cgacgatcag cccgtttaac gcatggctca tcatgcgtgg
      841 tgcagttacc ctgcccctgc ggatgcggca gcacaatgcc aacgcattgc aggtggcaaa
      901 gtatcttcag acggtaaagg gtgtccgctt tgtggcatac cccggactgg aaactcacaa
      961 gggacacgag atcgccgcag aacagatgca tggcggtttc ggcggcgtgc tgtcctttgg
     1021 gctgcatgcc gatcacgaca cctataaccg ttttgtcagc catctgaagg tgattacctc
     1081 ggcggtttcg ctaggacatg acaagagcct aatcgtcttt ctgggcgaaa atgacgaacg
     1141 gcagtatctg tatcccgagg aattccacgg cggatttttc cggttcagcg tgggcattga
     1201 ggacacggaa gatattatcg ccgatctgga acaggcattt gcaaaggagg gactgctgta
     1261 atggcgttgc acgcattttc cagaacggaa ctgctggtgg gaaagccggc aatggaacgg
     1321 ctggcacagg cacgggtggc agtgttcggc atcggcggtg taggcggcta taccgtagag
     1381 gctctggccc gcagcggcgt gggagcactg gatctcattg atgacgaccg cgtgtgtctg
     1441 accaacatca accggcagat ctatgccact cgaaagacgg tggggaaata caaggtcgag
     1501 gtggcgaaag aacgcattct ccagatcaat ccggcttgtc aggtagaaac ccactgtgcc
     1561 ttttacatgc cggacactgc cgcacagttt gatttccgcc agtacgatta tgtggtagat
     1621 gccattgaca ccgtcaccgg caagctggaa atgatagagc aggcgtatcg tgccggcacg
     1681 ccgatcatca gttccatggg agccggcaac aaaatggacc ccactgcctt tgaagtggca
     1741 gacatttacg aaacctctgt ctgcccgctg gcaaaggtca tgcggcacga actgcgaaag
     1801 cgtgggattc ccaagctaaa agtggtctac tccaaggaaa agcccattgc cccgaaagag
     1861 gacatggcaa acagctgtaa ggcccattgc atctgtccgc cgggcactgc ccgcaagtgt
     1921 acccagcggc ggcaggttcc cggcagcaat gcctttgttc cgtcggtggt ggggctgatc
     1981 attgccggag aagtggtgaa agatctgatc ggagagatcg agtaagggca gcagtgcaca
     2041 caaaacaaac cgctctcatt gacgatatgc aaaagaaggc tgtacacttt aaaatcgtgt
     2101 acagccttct ttttgtcaat tggtgttgtt caagaatttt gttgcagatt tccctagtgt
     2161 tcccccacac aaaacaaaac acgcagttcc gaaaaatctt cgaaactgcg tgttgattct
     2221 ggtctgagtg acccgacttg aacgggcggc ctctaccacc ccaaggtagc acgctaccaa
     2281 ctgcgccaca cccagataaa gtgctttctg cacaccagca taaataatta tagcatgttt
     2341 tttctgggtt gtcaatgatt gcgtagaaaa ctatttgtga tatggataaa tcaacaaaca
     2401 aaatgtattt ctgaaaatga atctgccgtt ccgcacgatc gccgctcctt attattgcga
     2461 agtcgcgtta ttgcgaaacc gcaggctgtt ttcgccgcca ttttcccgtg cggtattcca
     2521 gaaatgaaat cagccagttg cagaaccagc cgatgggcac ggcacaccac accataaaaa
     2581 tgccaaaccg cggagccatt gtcacagcaa cgatcacgcg aatactaagg ttcaccagat
     2641 tggcgaccgt gaacattttc atatccccag ccccacgcag caagccgtcc actgccattt
     2701 taaaaccgat aaaacagaag aaccacccca taaaggtgag gtagccctcg cctacggaga
     2761 acgctgtttc tgtgccgtct gctcccagaa acagagcgac cagcgggcgt tccagcagtt
     2821 ccacgcaaac gcagatcagg acagcactgc aaagtaccat gaggttcgtg gcatgatacc
     2881 cttttaccac tcgttcttcc tgtccggcac ccagattctg ggcagtgtag gaggagagag
     2941 cgttgccgaa cgccgccata ggcacaatgc aaatggactc cacccgcatt gccgccgaaa
     3001 atccagccaa tgcctcggaa ccgaagctat ttatcaccga ctgcaccagc atcatgccaa
     3061 tggaaacggt ggactgctgt aaaatggagg gcagggcaat gcgtgtcatt tgcagcagtt
     3121 ccgtgtgact gaagatgccg gtttttccgc tggggaaacg cttcagttcc cgcagcagca
     3181 cgagaaagga aagcactgcc gaaatgccct gtgcgatcag cgttgcccat gccacaccgg
     3241 tcacgcccat gtttgccttt gtgacaagca ctatgtccag caccacgttg aacaccgagg
     3301 aaaagatcag aaaatataag ggaatacggg attttcccag tgcattgaac atggaggaga
     3361 acacgttgta caaaaacaaa aatggcagcc ccgcgaagta gatgtttaaa tagtccacgg
     3421 ccatgtccag cacatctgcc ggcgtttgga ggacttccat gatctgtcgg ctcaatgcca
     3481 gcccgaaacc gcccagtaga atgctgactc ccagaaatgc cagacaggcg gtgtaaactg
     3541 ccagcttcat gcgggggaat tcctttgatc cgaaatagcg gctcaccgcc accgaagccc
     3601 cgatgccgcc gccaatggca atgcagatga aaatgttggt cagggcgtag gaggctccca
     3661 ctgccgccag agcctgttct cctacgtatc tccccacaat ggcagaatcc atcagggtgt
     3721 aggtctgctg gaacagattc cccagaatca tcggcagggc aaacaccagc agtgcccgaa
     3781 acggtttctg ccgaatcaga tagtcgtttt ccatgatgtc atactacgga aacgccggaa
     3841 gcttttatgt acaagcttta tgcagcgttt ccttgctcct tgtcaaaaat ttagagtgtg
     3901 ttcccgtaaa atgcttttgc cgaaaagacg tgcattttat ttttatggaa gcgtactcta
     3961 tagtatagga cgatctccgt ggaaaagtca accacttcct gtgaataaaa aacgacaagg
     4021 gaaatgagtt gttgttattt gatattccta cgatgacact tcatacgacg aaaaaaccgg
     4081 cagcacgtca ggaaacttcc tgttccgtac tgccggtatt tgccttagct attcctccaa
     4141 atacgcaatc gaatcggtat aatacgtatt gttatagatg tcctccagca cgtaataaat
     4201 aaaggtatcc cccagttgct ctgctgcatc tgcatagccg acttccagac tgtcctgcac
     4261 ggtgaaaatc tcatcgtcca gcgtcatcgt ttccacatag ttgagatctt cgtcgtagat
     4321 gtcatagtac acctgaaacg tgtccccgtc acagatgtca tacaacccct tggctgccac
     4381 gctgtccgta tacaccggac gaacgccggc agcatatccc gacgggtgct ccgtatccca
     4441 gacaagcacg atttccgcat cgataccgtt atagacacac ggcacagaac cgcaggtgaa
     4501 aaagctgtcc ccgtcactgg tataggactc gtggaaaaac ggcacgacct gtccgtccag
     4561 tgccacccat gtctggtcgt attccacgat cagattgccg tagtcgtcga actcgcagta
     4621 atcgtccagc cccatatcca catagaatcc gtcatagacg gcaaacatct gcattgcggc
     4681 atcattgacg atctcccagt cttcttccga aagctccagc acacacctgc cgtccacctc
     4741 cgtggtttcc agcaggctca ggtctgcact ataggtttcg tagtaggatt cgtcatagcc
     4801 ggcatcagca tcgaaccact gaaagccgct gtattcgctg gcagctgcgt gattctgggt
     4861 attggaaaaa ccggaaacag agatctgtcc ccctgccata acattggcaa actgctccag
     4921 aaacgccgga tatgcatcat cataccccaa agtctgatac atctcctcca gcatatccag
     4981 tgcggataga tcataatagg ggaacaaaat ggataacccg ttgtccccgc tgccgttctc
     5041 cgtttcctga taatatacaa cacagtcgga aaccgcctgt tccagctggt ctgccgtttc
     5101 gctctggctg tgctgtgcat agtccagcag atcgatgtga tccagttctg actgatacac
     5161 cgcacgggta ttgctgcggc tctgggagat ccggcggtac tcccctgcgg aaagctgctg
     5221 cactgcatca tcggagaact ggctcaatgc cggcagcagc ttctgtgtca cctgctgcaa
     5281 atcaaacatg cccagcgtgg aatacatact gggcgaatcc cagccgcttt ctgtgatata
     5341 gtcgtcaatg atctgccgcc cgtaacgttt cggcggaatc ccacagtttt ccgaaagctt
     5401 tccgatccag tcggtatagt accagccgca ccccggctcc ggttcttcgg aggcaatcag
     5461 ataatcggca tacggtgccg ccatcaggca ggtttcaaag ttcgccatca ggcaggcatc
     5521 aaaccccagc atatccagat gcacactggc atcgcccaga gcacgggaca tttccgtcag
     5581 gctcatgctg tcgccgccga aattttcgtc atagccatag ccgccaacca caccaccgcc
     5641 gtggtcccac agcaccagcc cgtagtgatc ggctgcgtaa ttgctgctgc aatattggat
     5701 aaagtcggtc agcgtagcac tgtctgccat gttctgcatg cccagactgt cgtccaccag
     5761 ttccagaccg tcctctgtca cccgataccg ctggcagcgg tcatctgcaa ctaccgtatt
     5821 ttgccattct gctgtgccgc cagtctgcac gatcacattc aggttgccgg cggtatagcc
     5881 atacatgatc tcgttgaggt cttccgtggc aaatccgcac tcggattcca gatcggaagc
     5941 acacatgtac agcatcaggg tataggtatc cgaagcattc ccctgttcct ccggcggaat
     6001 atcttcttcc cagtccagac agccgactgt cactaagctc agtactgccg ccatgcaaac
     6061 tgcggcacat tttcgcaaaa ataccggcat aaacagttcc tttcgtcatt tctcattttt
     6121 tcattttcgg caataccgca caaagattgt gcgacagatg cgtcgtcaaa tttttcgtca
     6181 ga
//
LOCUS       DAWBGR010000157         5996 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3329_length_5996_cov_3.287325, whole genome shotgun sequence.
ACCESSION   DAWBGR010000157 DAWBGR010000000
VERSION     DAWBGR010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3329_length_5996_cov_3.287325"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1510..1519
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1651 a         1504 c         1103 g         1728 t
ORIGIN      
        1 tattttattc ctttgaggtt agggtgtcaa gttttattat actacatcaa ccacaaaaca
       61 acataattct acaaccactt tcagacaata cagcagactg tcactcttac tttattttat
      121 agtaagcaaa ttttcccttt ccaacgagca aaagctttcc ttcttccgtc atcttaacaa
      181 gcagacgata tgcctgcccc tgattaagat gcaaaagctg cattacatcc tttctagtta
      241 ctttgccttg attatttttt gcaaacttca aaacaagttc tggataacga atccgatcaa
      301 tccctgtctg ccgcacgtat ccaattacat tgtccgcatt tttataaagt gctgcactca
      361 aaatataagc tctgctgttt ccatttccgt ccgcttccac aagtccggat tcaactaatt
      421 tttcaacggt tgtgcgtact ttgtactcgt tgagatgaat catatgtgca agttctccaa
      481 gccgcagtct tcgattggat ttcagagcat ttaatatcag caatgcattg atagataatg
      541 gtctgccgat cttgctttgt tcttctgcaa tcattttaat aaacgcctta tccggcaaac
      601 ttttaggaat aaatagtttt acttgatttg tattggattc tgaatagtcc ggcaatgatt
      661 ttccatagga aagtgagcct tcaaaaatac gatctacgcc tcgtccagta cgctctgcaa
      721 gaccgattcg ctttaataca tcagcaagtg caggatttct tccatgtggt tcggctgtca
      781 ataaattttc aattgttaca ccttcaataa atccacccgg attactgatg gtcaaaccat
      841 catcatccag taaaacacga acacgtccca acatcgtata atcccgatgg cagaaagcat
      901 ttaccaatgc ctctcgaaat gcccttttat caaaatccgg aatggaaatt cgaaacagtc
      961 cttcttccat ttcttttgta tggtttctgg catccatata tgattctatt ttctcaaaag
     1021 ccatcaatat cggcaataca aaggattcat ttactgctac atccgttccc tgtagttctt
     1081 gaaaggctgc ttcagcagtc ggaacaagcg ttgaaagccg ttctcggcgt cctattagta
     1141 acataccaca gtaagtagga acaaaagtac cattttgttc tactgccaaa cgaagtgcaa
     1201 aatctaattc cgtatcatca agatcaagca aatttttttc accatgataa ttttgtataa
     1261 tgttacgaag atgttctcgt tctaccggat ctaaatctga atatactgcc tccggaacag
     1321 gctgagcaga ataatccagc atacgcaaag aagaaagccg tgtattcatt tcatatggat
     1381 acatcgggat attttccggc tctccatcta attttatgcg tcttctcaga acttttccat
     1441 ttgaagcagc aacaatcgct gtatatttcg gaactgttac gatcaagtac tttacatcat
     1501 ctgcatccan nnnnnnnnng atgcagtcct gtaatatcac cattatcttc tacaccaaga
     1561 aaaaatttac caccgtcagt attcgcaaaa gcaactacac tttcgattat cacatcatct
     1621 gataactttt tcagatcgct cttgaattca accgtcatat tttctcgaaa tccaatattc
     1681 attttaacac tccttttacg ctcggtgagc attgaatcaa attcattata acacgaccga
     1741 gcgttaaaag caagcgttaa acgataaaaa attttatttg catttgaaaa aagcacggaa
     1801 tttcggaaca tttatagaat ttggtttaac actcgctaac gctcgcttcg tgcgttaaac
     1861 cgagtgttaa aatgactttg gctcattttc caatatattt taaggataga ttttcaaaaa
     1921 gcacatcacc ttcctcactc ctaaaaagcc ataaaacaac cagcctgaga ttccaatcgc
     1981 agaacctcag gctggtgtta tctttatata ccgtgaaaaa agtgacacac tttcctcaaa
     2041 tcacatcccc tgcaatatct cctccactcg ttcttccagc tcgtccgtct cataatcgtt
     2101 ttcccgcaag acccggatcg ccaggtcata atatcgcttg gcattttcct tatcccctgc
     2161 tttgtggtaa ttgtctccta ccataagata tgcccattgc atttgttcat gcagttcccc
     2221 gaaattcttc tgaaaacatt ccaacgcctc agctgcaacg atgttacaat cctggaattc
     2281 tcccaattct gacaacacag agctataccg catcaacaaa tttgcaagtg taacttcatt
     2341 gcacgcatca tgctccatca aaatttccaa cgcctgttcc atagcagctt tgcatttttc
     2401 cagcagttcc cggcttggct cagcgttgta ccagtcgatc aatgcacagg cataagtagc
     2461 ccagatctct ccggtttctt catgttcctc tccatagagc tgatttgcca ttgcgattgc
     2521 tttctcatag ttttcttcca tttcagaaaa ttgtttcatt ttcaaattgc agaatgccat
     2581 atcagtttga aaatgcacaa gctttgtatt tactgtcacg gattcttcct gcatttccac
     2641 ttgaaacaat tcttgttctt tttgataaca cgccattgca cgggaaaact gctcacaatc
     2701 ccgataaatg tcccccaact ctgaccaaaa tgatatatct tgttcctgtt ccgccgtttg
     2761 ttctgcaaag cactggtttg cataaaacac cgttttttcg atataaaaag atttattgca
     2821 atcgattcga taaatatttt ccaaccagat gaaattggaa tatgctaaag caagtttcat
     2881 aggctctccg atttcttccg caagctgtat gatctgttct acatacaact ttgcctggtc
     2941 aaagtcacgt tcttcgtata tatattcaaa taatactcct tttaaaacag aaaattgctc
     3001 cgatgcagac aactgcaatt ccgctaattc tgctgccgct ttttcgctga tctctgtatc
     3061 atgcagttta atggcaaaat cctgaataaa agataacgta tttatcagtc gaacccttga
     3121 gtcttcctcc tgtctcagaa tccgaagttc ctgctgtgct gcccataggg catcctcaca
     3181 acgattttct cccagataca gtcgataaca catcatagca aagaaaaatg ataattccgg
     3241 gtcgtcgccc tgaatatgat gtgccatatg atccagccac tgcacagata aaaaccgttg
     3301 ttctgcattt tcggattctt caatataacc agcaacactg ccagcaagtt ccaccatatt
     3361 tttgcattcc ctgctgttcg gacgcagttt ttcacacagc acttctacaa tcagcggatg
     3421 agtggaaaga atgcacattt catcctgata cacttcggag atccagccgc tttgaatcag
     3481 ccgatccagg ctgctgatct ctgcctcgtt caagtcactg atgacttcaa aatactgccg
     3541 ttccattgac gttgcaccga ccagggcagc acagcgaagc acctgctttt ccgattcgga
     3601 caaatccagc accgagaaca ggatctccat atgatggaac gctgtttttt cccgaagtgt
     3661 actgtctttc agctgacgca ctttaccgtc atctccggaa atgccttctg cacagagttt
     3721 ctcatacatt tcacgggagg tgagctgatg cagtgccatt tgctttgcca ccagttccac
     3781 tgccatggta tgtccgttca gtgccttcaa tatggcatcg gtgtacaagt ccggctctcg
     3841 ctttgtatag tacgccatca tctgatacaa cgctgattcg tcagacaaaa tgtccacctc
     3901 atactgggga aaaataccgt cataatccac tctggaggtc accagaatgg ggcaaggcag
     3961 ttctgtcaga aagtccaggt tctcgcagtc accagtatca aaattgtcca gcaggatcag
     4021 atgccttggc gtacagattt gatgcagtac ctgcaatttc cgttccagat atgccgcatc
     4081 actttccccc tcagagcgtg tacaatgcac tatgggaaaa ttggtatcgc tgagaatggt
     4141 gctctctata ctatccagaa accgaacaaa cacaacggca tcatactcct ccgcatacgt
     4201 catagcatag tgctttgcca gttctgtttt tccgataccg ccgacaccgt ggagcagcag
     4261 gaacttttcc tgctgcaaaa gggtatggat ctcctgcaat tcgatatccc gtccgcaaaa
     4321 gtaattgtta cccaagggca gtttggtttg cagataaggc tttggctgtt gaaatgaatc
     4381 cagaaaaggt tcgatctgtg tcagaagttc cgcacaggag gcataccgct tttccggatt
     4441 ggcagccaag gtctttcgca gcagctccgt gaccatgcgt ctgatttccg gagaagttcc
     4501 ggcaagcacc gagtccggga actccgtatc ccataccgca cgatttctcc tgtcctttac
     4561 ttccggagca cggcgaaaca acaggtacag caacaatccg cccagcgtat agacatcgga
     4621 cacaacgccg atccttctgc ggttttgctg taacacttcc ggtgctgccc actttgccgt
     4681 acaggagatc actgcatcag aaagccggct tttttcgatg gcactgtcaa aatcaaacag
     4741 catcaccagt tccggcgttt ccggatagag cagtacgttg gcaggcttta agtccagata
     4801 gagatatcca tgctgatgca gattgtccag ctgctgtgcc actgcacgca gtacccgaaa
     4861 tgtgtcataa agcgacaact cctgctgttc cagcggaatg ctgttctgac agttcatagc
     4921 aatataccgg gtgccgtttc cggcaaaaat gccctggaca ttgctgatac tgttgcagct
     4981 ttccggaaac tgccggaact gcatctgctt ttgatatccc cagataaagc tttgcatctg
     5041 ccgttgaaaa gcttcttcgt ctgccgcaag caaattgccg tcctgctgcc gaatcaggtg
     5101 tagattttcc ggacagaact ctttcagccg gagatagaat tcgttttgtt ctccgtccct
     5161 gcaaaccgcc gtatagacca gacagctggc tcctgttccg atcagttcct gaatctgcac
     5221 tgtgatctgc tgctgggttc caatggcgga aagggtcaga tgtgtaccga ctggcagtgg
     5281 cgttcgcatg gtaagttcct cttttcaaaa gttgtgatat tgatttatga cggcatctgt
     5341 aaaaggtatt cctgcaattc ctgcaatgtc tgaaatcgcc ttttccaatt gctacacaat
     5401 gttttctgat aaaattccga gtgtgacgga aagacagcaa accgtcttcg ctccagccat
     5461 gtcaccggtt ttccctccca ttgaaaatac agatagctgc cgatcaaata gacatagctg
     5521 ctttctgtgt aaactgcctt ctgcctgcat tccggtgcga gcagtgacca caaagggata
     5581 cccgaaaaaa atggtacgtg ctgtttcaaa aaaactgccg gggaaacgga ctccttttgc
     5641 aaaacagaga tcagttccat tgcctggcaa acacattcct gcaatcccat cacctcccga
     5701 ctgtcatcgt aacacaacgc catacggcat ttgtaacaac cacttgaaaa gtcattgtta
     5761 caaacccatt tcttcctatg atacaatgaa tcatgcggaa atctctccgc caatttacaa
     5821 caggaggaaa aaattatatg aaaccatttc aggaaacaaa ctacattgcc aaagacaaag
     5881 aaaacgaaca atggaaaagc agtctgccgc cggaagaagt caaggaaaag atgctggcac
     5941 agcgtggatt tactcaaggc aataccgcac aaagagcgtc tggcgacttt gcacgc
//
LOCUS       DAWBGR010000158         5965 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3346_length_5965_cov_4.240271, whole genome shotgun sequence.
ACCESSION   DAWBGR010000158 DAWBGR010000000
VERSION     DAWBGR010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3346_length_5965_cov_4.240271"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1560 a         1450 c         1547 g         1408 t
ORIGIN      
        1 gggggaccgc cgttaggcgg tggaggggtt tctacgtaac gttcaacgtc aaacaaaaga
       61 ttttacatca gtggaagatt ctctgtataa tcttcgtgta atgcttttgc aatgtagtac
      121 aagtgcaacg cgaactcttt tctttttttg cttcttttta gcaaagaaaa aaagaagcgt
      181 aactctttgt gaaaccggtt gcttgctgta gcgaattaca cggaagaacc cctccaccag
      241 ctttgctggt ccccctcccc tttcagggga ggctttttat tcaggctctt ttgaaaagcg
      301 aagctatcct tccccacacg ccaacaaaaa cagcccattt ccggcagaca caccggaaac
      361 gggctgtttc ttatttatca gatgagagaa tgatacaatc gaacgcttcg attagtatgc
      421 aacaccctgc cacttcatag catcagcaac cttcaggaag ccggcgatgt ttgcaccaac
      481 aaccagattg ccctctacgt cgtactcctt ggaacgatcg tatgcgttgt ggaagatgtt
      541 gatcatgatc tgcttcagct tagcgtcaac ttcttcgaat gtccaggaca gacgctcgct
      601 gttctgggac atttccagac cagaggtagc tacgccgcct gcgttagcag cctttgccgg
      661 tccgaacaga atgccggcag ccaggaactt gtcgatagct tccggagtag acggcatgtt
      721 tgcaccttct gcaacaacct gaacgccgtt cttcagcagg atctcagcag attcgccgtc
      781 gatctcgttc tgggttgcac acggcagagc aacgtcacac ttgatagtcc agatgccctt
      841 gcagccttcg gtataggtag caccttctac gcggtttaca tattccttga tgcgtccgcg
      901 ttctacttcc ttgatctgct ttacaacgtc cagcttgatg ccgttcggat cgtagatgta
      961 gccgttggag tcagacagtg caacaacctt tgcacccagt tcctgagcct tttcagttgc
     1021 atagatcgca acgttgccgg aaccggaaac aacaacagtc ttgcccttga tgctgtcatt
     1081 cttcatgcag ctcatcattt cttctgtgaa gtacagcaga ccataaccgg tagcttctgt
     1141 tctggccaga gaaccgccgt aggacagacc cttaccagtc agaacgccgg tgaattcgtt
     1201 acgcagtctt ttgtactgac cgaacatgta accgatctca cgagcacctg taccgatgtc
     1261 gccagccgga acgtcggtgt cagcaccgat gtgcttgcac agctctgtca tgaagctctg
     1321 gcagaagcgc atgatctcgt tgtcagactt acccttcggg tcgaagtcag aaccgccctt
     1381 gccgccgccc atcggcagtg tggtcaggct gttcttgaag atctgctcga agcccaggaa
     1441 cttcagaatg cttgcattta cggacgggtg cagtctcaga ccacccttgt acggaccgat
     1501 tgcagagttg aactgtacac ggtagccacg gtttacctgt acgttgccgt tgtcgtcaac
     1561 ccaggataca cggaactgaa tcatgcgctc cggctcaaca atacgatcaa tgacaccgtt
     1621 tgtttcatag gacggatcct ttgcaacaac cggctccaga gacatcagga cttcgcctac
     1681 tgcctgcagg aattccggct cattgctgtt cttcgctacg accttgtcgt atacaccctg
     1741 aagatatgcg ttcttaaatg taaatgccat gggaatctcc tcctgttgga taaaaaatta
     1801 aaatgtagca aaacattctg tcaagaatac aggtgatcct gccgctgcga gaggcgtttt
     1861 gccggcaaac tgccctgcaa aatcccgcct gtgtgtgttg gcgatgatcc catatcccca
     1921 cgctgtccaa actcaaaatg tatgtgatgt ctgtgcattc gcttcaaaca cctgtacacg
     1981 cacttcgaac aatatctatt atacaccatt tggctatgtt tttcaatgca aattttgcag
     2041 atttttgcac tttcttcata tcgaaatccg agaaaccgta ttttcaaaag aaatcctgct
     2101 ttttcgtttg aattgcaaat tttgaaacgc gaaaagaatc aaattgcaaa gcggacagcc
     2161 gcatggtctt ttccgcggga cggaggaacc gttcccgctg tactgccgtc accggagcaa
     2221 agaaaaaagc cctgcctttg tggaaaaggc agagccaaaa gaagcatttt ctgcaaagca
     2281 ggcagctggt cgtagaacaa aaaaataaaa attcttgggc gatgccgcag agagccgttc
     2341 ggaaacgccg tgcggatcgc cgcagataaa acagctgtca gctgcaaaaa agcggaacgc
     2401 agcacgttcc ggaatgccgt tctcattccg aaagcatctg ttttgctcca aaaaacatta
     2461 tatcgaaaaa agaccggatt gtcaagcaaa tttcaatttt tcccgaaata tgcgaaaaaa
     2521 caaacacgtt ttttgtcaga tctccatgaa acgcaggaga tcggcgggag ttcgggcgag
     2581 atcgtctgct ccggcacgtt ccagctcctg ttctccgcgg aatccccaga cgcagccaat
     2641 ggtacgcacg ccggcatttt tgcctgtctg aatgtccacg tcggaatcgc cgatcatcac
     2701 cgcctgtgcc ttggacacgc cggcagtttt caggatctgt tccacgatcg ccggagccgg
     2761 ctttttcggg cggtcgtcgc tgctgccgaa gatctggtcg aagcagtcgc tgccgaaata
     2821 gtgccggaca atggtttcca caaagggctg tgtcttgttg gaagccaccg ccagatgtac
     2881 gccggcagct ttcagctgtg ccagcaccgc gggaatgccg tcataaggag ccgttttgtc
     2941 tagacagtgc tggctatagt agtcgttgaa ttccgcaaaa aaagtttcta cttctgcggc
     3001 aggcgtgtcc ggcggcagac agcgttcgca gagcttgtgc acgccgtttc ccacaaaatg
     3061 gtagtaggca tcgatggaat gcgtgggcag gtgccgctgt tccagcaggt ggttggcggt
     3121 gccgcccaga tcggcgatgg agttgacaag tgtgccgtcc agatcaaaaa tggcaagctg
     3181 atacataaaa catgctcctt tggtcggaac cgcatggcag ttccggtttt ggtgcgggca
     3241 gcctgtgcgg gctgtccatg catttattat agtacatgct gggggatttg tcaaaccaag
     3301 tagacttttc agcgtgcttt ttggggacac ttcttaaaaa ataacataat tgagaaaaaa
     3361 agtgtctttt tttcttgaca aacccgtggg aatttgatac tctatagaat ggaagcgaaa
     3421 agaagaaaca tcgtatatga ttcgcaatag gaaaggggaa atgtgtcatg aaacgaaaaa
     3481 ccatttgttt tctggcagcc tttggggcaa ttctgggcgg cagcgtgctg ttcggcggaa
     3541 cggcttctgc ggaagaagcg gaagtgacgg acgtgcctgt agcaacagag gtcacagagg
     3601 tcacggttcc ggcagtggaa aattccggct ggcaggacga ggacggggta cgccgctatt
     3661 atgacgaatc cggaaacttt ctgacgggtg aacaggaaat tgacggggcg tattatctgt
     3721 tcgactatga cggcgtgcag aaaaccggct ggcggacggt caacggcgtg cggcggtatt
     3781 acgatccgga aaccggaaac atcgtcagcg gctgggtgga ttactgcgat taccggtatt
     3841 atacagatgc agacaccggc aaaaaaaccg gagaactgca agacggcgaa gaacggtatc
     3901 tgctggacgc agaaaccgga cagcagcaac tggggctgtg cacattttcg gatcacactg
     3961 cttcgtatta tgatgcaaac ggcaaaccag tttccggctg ggtgaaggac aagggaagaa
     4021 cataccattt caatagcaag catctgatgc agaccggctg gcaggacttc ggcggcaaaa
     4081 ggtactactt tgcttcttcc ggtgtcatgc agaccggctg gcaggagctc tccggtgcaa
     4141 agtattattt tgattccgac ggtgcgatgc acaagggatt tctgcggatg aacgagaata
     4201 catattatct gaacagtcag ggaaaaatgg cgaaaagctg gcagactgta aacggacaga
     4261 aatattactt cgacaacaac ggcgttatgc agaccgactg gaagatgatc ggcggcaagc
     4321 tgtatttctt cggcgataac ggcatcatgc agaaaaacaa ggagatcttc tgctactatg
     4381 atgaaaagca ctacggcgac tattatttgc aggcagacgg cactgcgatc tccatggcat
     4441 gctaccggct gaatcaggct tcgctgaagc cgcataccag ttttgttgtc tataaccggc
     4501 agaaaagttc gcacagccag tggacttcct atatttccgc aaaggacaag cagatcctgc
     4561 aaaaatttat ccagcagcat ttcaaggcag gcatgacgcg ggaagagcag ctctggacga
     4621 ccatggagtg gattcacaac aacgtggaat atgcgtatgt acagaacggt gcatgggcac
     4681 agatcacgaa caagacctat gtcgatgccg ttttcacata tcggaaggga cagtgcattc
     4741 agtacaacgc ggcaatggca gcgatgatgg catatctggg ctatgatgtc aatctggttc
     4801 agggctatgt aatgagcgaa ggaaaccagc atttctggtg tgaagtgcac atcaacggca
     4861 agacctatgt catggaaacc ggaaatgcag gcaaaaacgg agactggatg cacttcctgg
     4921 aaccgtacag tgaagcaaca gagtatattc agcattaagg aggttttacc atgaaaaaaa
     4981 gacaaagact tctcgctgca ctgaccgcag cgggaatgac agcggcggca gttcccgctg
     5041 tggcagtatt cgcagacgac agcgttgccg ttactcccag caagtatttg caggatcaaa
     5101 agggcacact gaccatgacg gtttccggtg atcgggacgt tcgggtgaag atcgagaagg
     5161 acacgctgga cggcgttatc acgtactacg acaccacact ggaaaacgac ggtacctacg
     5221 acttcacgct ggacagctgt gagtataatg tgaatacgga aacctatgat tcctctttca
     5281 ccgtcagcgt gtgggacaag gcagacagca gttgttccta tacagaagaa catctggtcg
     5341 tcatggatcc gggctttacc cagaatgtgg aacggagcca gtttgactgg aacctcactt
     5401 tgcaggaggg ggacaagcag gacgtttccg cagtggtgac agatgcttct gtgcaggacg
     5461 gcatctgggc aggcggcaca gacctgacca tgcagtatgt gccgtatacg ctgggagatg
     5521 tggacaacga cggctttatc gattccactg acgtgttcga actgctgctg tacgtggcaa
     5581 agaaaagttc cggtcagaag aatgcgactt tcacaggaga tgcgtcctct atggcggaaa
     5641 ccgtggcagc caatgcggca gatattgaca aaagcgggga catggacagt acagatcttt
     5701 actatctgct gtattacatc gccctgaacg gtgccggcgt ttcgacaaac tgggaagaga
     5761 ttctggcaaa ctaaggcaat gccgcatatt agaagcacat agcagcacaa caccgcatgc
     5821 cctttccgga tctcttccgg ttaggcaatg cggtgttttt tcataggctg aatggagcag
     5881 ggacagattt cgcaggaacc acggaaatca attttcaaaa atatgttgat ttaaattgtg
     5941 ccaaattatg aagtttgaca agtga
//
LOCUS       DAWBGR010000159         5959 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3350_length_5959_cov_2.991023, whole genome shotgun sequence.
ACCESSION   DAWBGR010000159 DAWBGR010000000
VERSION     DAWBGR010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3350_length_5959_cov_2.991023"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1083..1092
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1364..1373
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2027 a         1054 c         1326 g         1532 t
ORIGIN      
        1 gatgtaatgt tatcacacaa aaatatagca tcaaatattt atgctatgca aacatgtgaa
       61 gatatacgtg atacagatac aaatattgca tttttaccat ttcatcatat atttggatca
      121 acatgtataa taatgatgat agcttcagga gtaaaaacag tattcccaga tggattaaga
      181 tatataaaga aaaacttaaa tgaatataaa gttacaattt ttgttggtgt gccaatatta
      241 gttgaagcaa tatataaaac tataatgaaa gaagttgaaa aacaacgaat cacaaaagtc
      301 ataacccaag acttcggaat ctccatgcac ctgaaaggca gtcactacat agaagaagct
      361 gtcgctttgg cacttcaaga ttcaagcatt tttgtaatgg agatgtatca agccatagca
      421 gaaaagcaca acaaaagtgc tgccagcata gaacacacca tacgttatgc cattgaaatc
      481 acctatgaca aaaacaaatt gaatcacttc tttccacatt ccttcggtcg gccttccaat
      541 tctgaggtga tctggacaat tgctgaaaac gtcaaaaggg gcatgttgat tcacaatatg
      601 tgtgcggtga cttgttcaat aagcgcaagt tagccgcaaa tctgaggtga aagatcagct
      661 tcttcaagcg aattctaccg attgaatcaa ctcaaaagga aaaacatgac caacgatcta
      721 accttcggtc agttcatttc tgaacaaaga caaaagcatt ctatggaaag tcaggagctt
      781 gcaaaagcgc ttgggatttc aggcgcctat ctttctcaat tggaacgagg tgtccggacg
      841 aatcccagca cagagcttct ggacaaaatc gcaatggttt tgtgtctgaa caaaattgaa
      901 acagaaacat tgtacgatct gtatgcaaag gtaagcgggc aaattagtcc tgatcttgcc
      961 gtatatgttt cctccagtga aacagtgcgg caagcactgc ggactgcccg tgatgccgat
     1021 gcaacagagg aagattggaa actattcata gaacagctga acaaatgaac aataattttg
     1081 cgnnnnnnnn nnagcaatcc gcaaaattat tgcagttgtt ttagctaggt agttaaaatt
     1141 gaggtgaacg aacaaatgtg aaagaaaggc aacataaatc acagcatcag gtacgttgtc
     1201 cgttttgtaa tggtaggtta tttgatattg tgacagatga aaatttggca cagcactatg
     1261 tgtcctgcat ggttgtagtc aaatgttgga agtgccgtca agaaatcaaa atcatatatt
     1321 ctgacttagt acagacacca cgtatataaa atatagagta ggannnnnnn nnnctttaaa
     1381 agcgggatgg agttttttat gccctttttt caagtatggc agtgcagcat tgccaagtat
     1441 ccatagcctt cgtttgattt aaaaccttat atatcaaacg gaggtgagaa aatgaagaaa
     1501 agcacagttc gtatttatga tacaatttca aaagaatatg ttgacattga agtcagtgag
     1561 gaagtctata catattacaa taggtcacaa tggaatatgg atgacaatga tgattcgttc
     1621 tataagcatg aaattcagtt ttcagcgtta attggcggca atgatggtac gtttgagaat
     1681 ttcagagaat ttagaattga aaacagcgtt gaaaaagagg tgataagtaa gcttttcacg
     1741 gaaaagcttt atgaatgtct caatttatta tcagcatcag atcgtcaact gatagagatg
     1801 ctgttttttg aggggaaaac agaacgagaa tgcgcagcat actatggcat cagccagaaa
     1861 aatatcaaca aaaagaaaag gaggattctg tgcgatctgc acaaactttt agaaagtata
     1921 aaataaaggg tatcaaattc ccgacttctt cccctataca agcgaaagga gataaaaatc
     1981 gaatttcatg tatcttgaaa attgaataca cagcatcggg aatacatgaa tcctgcgggc
     2041 ggaataaacc ccgtcagccg cagaagccat acgccacgac ctcaaaagag cgagcgagaa
     2101 atatgcagct tccaaatccg gaatagcttt tcggaacggc gatgaaacgc aggaggataa
     2161 tgctacttcc gtccagtcat agcacccctt ggtcggggaa tcaggcaatg agggcggctc
     2221 cgggagatcc tcggagggat gagatttcca tgagactgat acgctgtcag tcgtttccga
     2281 tgttccgccg tgacgggtgt taagaacaaa tatcacggaa aatcgtctga ggaatcagac
     2341 gaaggcaaaa cgttaaagtt ataccgcact gttttttgct aaggcaaatc aaatctatca
     2401 gtgcggatat aacgcaagtg caggagcatt ttttcctgca tttgcgttat atataatgaa
     2461 ttggagtgtg gtaagttgaa agagaagtta agctggaacg aaatcagaaa tctgattttg
     2521 catagaagaa aaaaatttca ttacggcgaa aattgccgaa aatgtatctg ggcagataca
     2581 agatcgggaa aaattttatg ttccaggtac aaatgtgtaa aggaaagagg aaaatgaaca
     2641 agtacggaca agccgaatat gcctatggga ttctccgaca gatttcagag aaactgcttg
     2701 ccgagggaat tctgacagaa gaacaactga aacagctgaa tgcgctaaac aaagagggct
     2761 gctttcgtca gttctgcaca gctttagcgg cgtgatttca gtgggtttga tgataccaat
     2821 tttcaagaaa aagtggtatt gtatgtagtg aaaaaatcag attggaggga gcaaagtgaa
     2881 tccaaacgta acggtaattc agccgacaat tacattggaa caggagcaga aaatccgggc
     2941 tgcagcatat tgtagagtca gttccgattc cacagatcaa ctgaattcgt tcatgacgca
     3001 gatgcggtac tatgaaaatt tccttgctga tagtaaaacg gaaacattga tttcggtata
     3061 cgctgatgaa ggcataacgg gcacccgaat ggataaacgt gaggagtttc agcggatgct
     3121 caaagactgc cgcagaggta aaattgatcg tatcatagca aaatcaataa gtcgtttcgc
     3181 acggaatacc aaggaatgcc tgaccgttct gcgggaacta aaatcacttg gaattacggt
     3241 actttttgag aaggaaaata tcgatacggc gaatatttca gatgaaatga tgatcacgct
     3301 gatgggtggt cttgcgcagg aagagtcggt atcgatttcc cagaatatgc ggtgggcaaa
     3361 tcgaaaacgc atggcagatg gaagttataa aatctcctgt ccgccctatg gtttttccat
     3421 ctgcaatgga cgattggaaa tcaatgaaac agaagcaaaa attgtcagaa atattttcac
     3481 atggtactta agcggctatg gaattacgaa aatcgcaaat atactgaatg atttgaaaat
     3541 tccaagcagt atcagtaaat cggcgtggtc attttttagc gtgaaatata ttttgacgaa
     3601 cgagagatac attggcgatg cggtttttca gaagacgtat aaaacgacaa ctttgccata
     3661 tatgaagaaa ataaatcatg gagagtatga aaaatattat gtttctggaa tcaatgacgc
     3721 cattgtggat aagttagatt ttgaaaaagt gcaggaattg ctggcgattc ggggacaaaa
     3781 agcacaatct gcatgctgtg aacatccgct gtccagaaag cttatctgcg ttttttgcgg
     3841 tgcaactttt aaaaggaaac gctgtcgtga aaaatattac tgggtatgta gaacgcacaa
     3901 tgagaatgcg gaaaagtgcg gcggacagcg gttgccagaa tcaaaaattt atgaaatgtt
     3961 tatgcgggtt tacaataagc tgaaatcgca atacagcatg atttttcctc ctataatttc
     4021 acagcttcag gagctgaaaa accgcaaatt cagcggcaat cagcagtaca tggagattgc
     4081 aaaggaaatc gccaaactta aggagcagaa tcacgtactt gcccggctga aaacaaaagg
     4141 atttctggac gaagcaaagt accttgagca aattgctgaa ctcaattcca agattaacaa
     4201 actcagccgt gaacagcata aaatcgtgcg ctccgatgat gaggacgata tgatagatca
     4261 aatcaaggat atcgcttcaa tcattgaaaa cggcatggag ctgatgacgg aattcaatga
     4321 gatcatgttc gaaagtctgg ttgagaaaat tattgtgatt aaccagaatg aactggaatt
     4381 ccatctgtac ggcggtctga aatttactga aaggctggtg taaaatatat gaaaaacaga
     4441 acaatcccat ttggctacat gatgcggaac ggagaaattc agccaaagcc aacggaaagt
     4501 aaagccgtgc agaatatatt tcaggcttac ttgaacggca gttccctgct tgccattgca
     4561 aattggatga gtagtcaagg cattccttac aatggcatcg attttgtgtg gaacaaaaac
     4621 atgataaagc ggattctgga aaatgaaaag tatctgggca gaaacggcta tcctgcaatc
     4681 attgatgaag atatgttttg cagagcaaat atgcggaaga aaattaaatc gactacagtc
     4741 gtcgagattt ctgaggagct tcaggcaatc cgcagtctta catattgtgc ggaatgcggt
     4801 catcggattt caagaatcgg cggcaatacg cactctgaaa aatgggactg ccgcaatccg
     4861 gaatgctcac gattcagcca cagaattaca gatcagatgc tgattggaat aatcgtcgct
     4921 gttctgaata cagtgattgc aaatcccaat ttgcttgatg ctgatgcaga aatcagcaga
     4981 tatacgccga gtattgagat aacccgtcag cagaatgaaa tcagccgctt gatggaaact
     5041 cctgatgttg attttgaaag tgcgaaagaa gaaatatgca agcttgcaga actgaaatat
     5101 gactgctgta cctacagcaa taaaccgcag aaaacaacgc agttgcgaga agttcttgca
     5161 agtcatgaac aactgaatac gcttgacatt ggcttactgc aatcctgtat ttcacggatt
     5221 ctggtaagcc atttttgcac cgttgaaatc gaatttatca atggcgttgt catcaaaaat
     5281 atcatagaaa ggaagatgca aaatgaccac agcgcccaat gtcagggtaa ttcctgcaaa
     5341 gccgcaaact acggaaaaca gggataaata ccatcagctt agagtggcgg catactgtcg
     5401 tgtatcgacg gaacaggagg agcagcaaaa cagctactag gttcagatcg catattatac
     5461 ggatttgatc aatcgcaaaa aggaatggac actggctggc atatttgccg acgaaggaat
     5521 ttccggcact caagcaaaga aaagaccgga atttttgaaa atgatacggc tgtgtaaaaa
     5581 gcagaaaatt gacattgtga ttaccaagtc aatatcaaga ttcgcccgaa acactgtgga
     5641 ttgcttggag tatgtgcggc aattaaaaga ccttggaatc ggcgtcatct tcgagaagga
     5701 aaacacatca acagcaaaag tgccgtaaat tcgcagttta cggcactttt agtttgatat
     5761 tgatgtttaa ctggtgtttg aatttatgaa acaataataa acaaaatatc acataagtca
     5821 atatagggta tctctgctga aaatttataa atttctgaac cattagttac cgtcatgttt
     5881 ctttcatttt ggtgccactt tgatattcgt aatgattgct ctgtctatga ctggtttttt
     5941 aaattgatct tgttccata
//
LOCUS       DAWBGR010000160         5956 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3351_length_5956_cov_3.879342, whole genome shotgun sequence.
ACCESSION   DAWBGR010000160 DAWBGR010000000
VERSION     DAWBGR010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3351_length_5956_cov_3.879342"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1511 a         1544 c         1449 g         1452 t
ORIGIN      
        1 agcattgatg cacgatattt tccataacaa cgacaacaaa cggaaataac cgccctgact
       61 gctaaacccg ggcggttatt caaaaacgct atgagggagc agaccgtcta ctggtatgct
      121 ctttctattt ctattatacg cgatttgcac cgacttgtca agtctttttt cgttgggcga
      181 ttgacagacg aacacagttc actctgcaac agttttcaca tacaaagcca aaaaagcacg
      241 cagtccatac agaccgcgtg caaattcctg gagctgttaa ccagattcga actggtgacc
      301 tcttccttac caaggaagtg ctctaccaac tgagctataa cagcatttct tttattgctc
      361 tcccgccgtt tttggcgaat caacgtatat tattatagca caaatgctcc gatttgtcaa
      421 tgtattcttg tcattttaaa aaaaagaaag tttttatatg atgtattttg ttttttcttg
      481 taaaacaaaa ctttcaatct gattatacga aaaagcatct gtaaaaataa agctcttttt
      541 tgtaaacaac gacgtttcct acaattcgaa acacaaaaaa ccgctgccag tttcacaaga
      601 gaaaccagca gcggtctttc attcgttttg tacagtatta tgaaaacacc gttttattcg
      661 caagtcacga tatatccgct gatattatcc ccggaagcgg tataggtgcc gctgggaatg
      721 gtaacctgta cgtcctttgt gccaccaatg cccacataga acacctgata ggtcttgccg
      781 tcagccgtga tctccagcgg gttgtccgca ctgtagctgt gtacacggtc gatgtattct
      841 tccagacaga ggttgtgttc ctgcatatag agagcgtggg gtacgcctac atagcggaat
      901 gtatatgtac ggctgtcctc accggttttt tcgtccttgc cgtccggata gcgtaccaca
      961 aagccatatt ctgcggcatg ttctgcgatc cagctgtact tcggatactt ttctgcatta
     1021 taatagtccg aagtgccgtc cccgaaattg attttcaggt tgaagctgcg gccgctgtga
     1081 taatcggaat agccgccacg gaatccggtg ctgccgtcct catagcggga atcctgatct
     1141 gccttgtccc gatagccgct gaacacctgc atgccgccaa gaccggtttc ggtttcaaaa
     1201 gcggacatca ttttgttcag ctgggcaatg gtttctgcgt ccagctgtac ttccatatcg
     1261 ctgacgctgt aggaactatt tcgtttctcg tagacacttt ccaacttggg atcaccgttg
     1321 gggaaatcgt agctgtgttc cttgttgatg agaatcagat tgccctgatc gacacccttt
     1381 gccgccagcg tgatctgctt cccgtctttt cggctggttt cctcggtgct ggttgtggtc
     1441 ttggtggtat cggttgccac gtccggctct gtggtctttt tgggcttatc tgttaccttt
     1501 tcggtctgct tcgtggttgc cgttgccaca gccgcattgc cggaatcctc aggctgcttc
     1561 cggcgttggc agctgtggaa gcaaagtgct ccgatcagca gcacgatcag cagcagcggc
     1621 acgaatactg ctgcgatccg gtcaaaccgg agttttcgct tgcggacatg actgcctcct
     1681 ttggaagaag cttttttatc catatccggt tttggctccg gctctttggc atgactgatc
     1741 ggttttttcg gttcgctcat gattgcaatt cctttcttgg tatcggcaat ccgcacatga
     1801 cattcacggg gattccctat gatttttcgc agactccgca gaatccttgc cgcggaaaat
     1861 gctgactaca ctagtatagc atattttccg gcacttttca agtcggaatt caggagaaaa
     1921 tccgaaagcg atcaccgata catacaaaaa ggctgcacag tccgaaaacc gtacagcctt
     1981 tccaaagaac ctttggtgcg gacgacagga cttgaacctg cacgccgaag caatagaacc
     2041 taaatctatc gtgtctgcca gtttcaccac gtccgcaggg aatgttttta gtataccacg
     2101 attttccgga tttgtcaagc acgtgcatca attttttccg aaacgacacc cccaaggcaa
     2161 acaagccaac cagacaaatg gacaaaaaac cagcgaaatt acgtgcattt caccttgtct
     2221 ggtgcaattc cagccgtcag acaaacagga acaaaaccag acaaacaggc tctaagcctc
     2281 ccttttcaga ggaggctatc atgaaaattg atttccatgt ccgagcaact cactgccttg
     2341 acgatttccc aaattcgtgt tacaatagta ccatactgaa aatttggagg aacgtaacat
     2401 gcgaatctta gtagttggca tgggactgat cggcggctct atctgcaaat cgataaaagc
     2461 agccacttcc catgccgttt acggatgtga cgtacagcag gcagttctgg acgatgccat
     2521 taccgacgga gcgatcgacg gcaccggcac tatgacagac gacacttatg acatgatcat
     2581 tctctgcctg caccagcgta tcgccattca aatggtacag gaagccctgc ctaaaattcc
     2641 ggcaggcacg attctggtag acacctgcgg tctgaaaggg cgtatggtgc gggatctgac
     2701 ctaccgctgc cggaaagccg gtgtgcgata tgtgggcaca caccctatgg ctggacggga
     2761 gaaaaacggc tactacgcca gcattcccaa cctgttccag aacgccaacc tcattgtcac
     2821 accagtggac ggcaccgatg aagcggctct ggacaccgtc aaggaactgg cagaccagat
     2881 gggcttcggc aagatcgtca ccgctacgcc aatgtggcac gacaacatga tcgcctatac
     2941 ttctcagctg gctcacgtgg tttccagctc ttatgtcaag gacttctgca tggacgatgc
     3001 cctgtctttc tccggcggca gctttcagga tatgacacgc gtggcaacca tggacgaatc
     3061 tatgtgggct gccctgtttc tggacaatcg ggacaacctg ctgtaccacc tcgatctgct
     3121 gatcgaaaac cttacggact accgcgatgc cctgaaacag cgggacgagg aacggctgca
     3181 atatctcatt gccgagggac ggcagattca ggaagaaaat gtccgtaaac gtcaggaaca
     3241 aaacgaactg caaaaaggag aaaccgcatg aactggacac aagttgatat ttacaccgcc
     3301 acgcctgcca ttgacctgct gagcgtccgg ctgcaggatc tggggatccg cggctgcatg
     3361 gtacaggacg cacaggattt tcaggagttt ctggaacaga aagacggcaa gtgggactat
     3421 ctggaggacg ggctcatgga actggcaagc tgtgaaacct gcgtcacggt ctacctgccg
     3481 gaagatgcac agggtgccga aatgctggca gctctgcgaa ccatgctggc acagatgaaa
     3541 gccgaggaca ccgaacacca gtacggcaga ctggaagctg tctgctccgg cattcgggag
     3601 gaggactggg cgaacaactg gaaacagtat ttcaagccat ttccggtggg aaacaagctg
     3661 tacatcaagc ccagctggga agaacccacc gcagaagccg ccggcagaac cattctggag
     3721 atcgatccgg caagcagctt cggcaccgga cagcaccaca ccacacggct ctgtctggaa
     3781 ctgatggaag atgagatcac ccctgccacc cgcctgctgg acttgggctg cggcagcggc
     3841 attctgttta tcggcggcat gctgctgggg gcaaaggaag cctatgccgt agacattgag
     3901 gaaaatgcca cccgcatcgc caaggaaaac gccgcagaaa atcacctgaa tccggaaaac
     3961 tataccgttt cctgcggcaa catcattacg gacaccgccc tgcgggaatc gatcggcagc
     4021 ggctatgatg tcatcacagc caacattgtg gcagatgtca tcaagggcat ggctccgctg
     4081 tttccgtcgt tcctgaaaga caacggcatt ctgatcctgt caggcatcat cacggaacgg
     4141 gcagacgaag tgctggacac tgtgactgcc tatgacttcg ccgtgctgga acggcgggaa
     4201 gcatccggct ggtgtgctgt caagctgcaa aagcttgccg gagccggcac ctgctgcgac
     4261 accctgcgga agtaaccatg gaactgctac agctggaata cttttatgca gtggcacaga
     4321 accagcacat gacacgcacg gcggagcagc tgcacattgc acagccctcg ctcacccaga
     4381 gtatccgccg gctggaaaag gaactgggcg tgcctctgtt ttaccgcagt ggcaggaaca
     4441 ttgccctgac cacatacggt gaggcactgc agaacgctct gacaccggtg ctgaaaactc
     4501 tgcaccagat tcctacgcaa ctacagcaaa tggctctgga acgggaaaag accattcggc
     4561 tgaatgtact ggcggcttcc acactggtga cacaggcact gatctcctat aaaaaagacc
     4621 atgacgggct gcatttccgg ctgattcaaa actcgcagtg cgaggacgca gacatcacgg
     4681 ttttcacgcg ggaaaatttt cagattccca cagataccaa ggaacggtat gagctgttta
     4741 aggaacgcat ttttctggca gttccacaca attcgcccta cgccagcgaa gaaagcgtcc
     4801 gcttacagga ctttgcccat gaggatttca tcagcctgtc cggttcccgc agtatccgca
     4861 cgatctgtga ccgctactgc atgcaggcgg gatttgtccc gcacatcatc tatgagagcg
     4921 acagtccgga cactgtcaaa aacctgatcg ccagcggact gggagtaggc ttctggccgc
     4981 actatacgtg gggcatggac agcatggagc agattcgcct gctgcctatt gcggaaccta
     5041 cctgccagcg tgccattatc ctacagctgc accgccacgc cagagggcag gaggcagtgt
     5101 gcagctttta cgcctttctg agccgctatc tgcggcagct gaaagccgcg gaacagcatt
     5161 aagaaaacaa accaccgcat cactccctcg gatttctccg gcaggtgatg cggtggtttt
     5221 tgtttgacat ttttgtcgaa tgaagatttg cagtgttctg ctatccttgg caaatttgat
     5281 cgtatacatt tactataatc ataaaaatag taacgacaat caccgcaaga acaaaaagaa
     5341 ataaccgccc cacagcttcc aactaaaggc ggttattttt ttaccaacta gtgggagcag
     5401 accgtctact ggtatgctct ttctatttct attatacgcc cgaacgccgc atttgtcaag
     5461 ggcttttttg tgggaaacgc ggctaatctc atttgacatt tttgtcgaag tgtggtatac
     5521 taccatttga gggcatacct gaaacggtag gcggtcaagt cttttttgat ttttgctccc
     5581 actgctcatt tgaacagacg ggaggtgatt cgtatgatgg atcaatatgt cacctttggt
     5641 gatctgctga agatttgcag tgttctgcta tccttggcaa atttgatcgt atatatttac
     5701 tataatcata aaaatagtaa tgacaatcac cgcaaaaaca aaaagaaata accgcccctg
     5761 agttccccaa cttgtggcgg ttaagtcttt ttaactacta acagtgggag cagaccgtct
     5821 actggtatgc tctttctatt tctattatac gccagaacgc cgcatttgtc aagggctttt
     5881 ttgtggaaaa tataagagcc tgtctgcgtt gtcaatagat gtgacccatt ggatgaaaag
     5941 atgccagaga taaggt
//
LOCUS       DAWBGR010000161         5888 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3390_length_5888_cov_5.025716, whole genome shotgun sequence.
ACCESSION   DAWBGR010000161 DAWBGR010000000
VERSION     DAWBGR010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3390_length_5888_cov_5.025716"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1403 a         1562 c         1665 g         1258 t
ORIGIN      
        1 tggcgacttt gcacgccatc tgcgtgcaga ttttccgtca tattgcccag aaatctgaca
       61 acgcacctta cgcacaagct ttgtgcggtg ttgccctaac agaaaggaag atgatacaaa
      121 tgcgaagatg cacacgtgcc gcggctctgc tgaccgcagt ctgtctgggt gtcagtgtcc
      181 tgccgccggt gtctgtttct gcggcggact tttccgccaa ctacgccgag gctctccaga
      241 agtccatgta cttttatgaa tgccagcagg caggaccgct tccggaatgg aacgaagtgg
      301 agtggcgtgc cgattccacc atgtccgacg aaatcaccgg cggctggtac gatgccggcg
      361 accatgtcaa gttcaatctg cctatggcgt attccgcttc catgctggcg tggggactgt
      421 accagtaccc tgacgggctg gagcagaccg gcgaactgga aacctatgtg aacaacctct
      481 cttttgtgct ggactatctc gccgcctgtg acgagggcga caccgccgtt tatcaggtgg
      541 gcaacggaca gatggatcac acctggtggg ggccggtgga gctgctggaa tacggcatga
      601 aggacaacgg ggcagactac gccaaagcca gagccgtact ccgcggaacg gattccgctg
      661 tctgcggtga aatggcggcg gcactggcgg ctggctcctg tgccctcaag ggcagaagcg
      721 acaagaccgg cgactatctg aaacacgccg agaacctgtt ccggctggca gacgaaacac
      781 ggagcgacga agcctataat aatagtgatg ccagcggatt ctaccgttcg tcccacttct
      841 atgacgaact gttctacgcg gcaaactggc tgtacatcgc caccggcgag cagtcctatc
      901 tggacaaggc gacttcctac attcccaatc tgggaaagga actgggctct gacgaactga
      961 aatattcgtg gggcatgtgc tgggacgacg tgatgcaggg cggtctgctc ctgtacgcga
     1021 tcaacaccaa cgacagcttc tacatcggac gggtacagaa gcacctgaac tactggacgg
     1081 actctgtaaa aacgctggac ggcggtgcaa agtggctgac cacctggggc tgtctgcggt
     1141 acgccaccac tgccggattt ctggcaagcg tggcgtgtga cactgtgctg aaaggcaccg
     1201 acacgacaaa gtatcagcag ttctacgaag accagatcaa ctactgtctg ggggacaatc
     1261 cggacgggca gagtttcatg gtgggctacg gagaaaaatc cccgcagaat ccccaccacc
     1321 ggacggctca cggctcgtgg aaaaatgcac tggatacgcc ggaaaccaac cgccacatct
     1381 tatatggagc actggtgggc ggccccaacg aggacggttc ctacgaggac gaccggcaga
     1441 actacatcaa caacgaggta gcatgcgact acaacgccgg tttcacggca ctgctgtgca
     1501 agatgaccga agcctacggc ggcacaccgg atccggattt tccggaaccg gaaacgcggg
     1561 acaaggaatt ctatgtggaa accaagctga cggaaacttc cggcggcgtg acgctgagtt
     1621 tcaagctgac caaccactcg gcatggccgg cacggatcga ggacaacttg tcctatcggt
     1681 attacatgga tctgtctgaa gtcatcaacg ccggcttcca gcccggcgat gtagtcatgc
     1741 gggtagaccg cgatcaggcg aagatgtacg acgactacac gccggcacag atctcggaag
     1801 tgcagcacta caaggacaac atctactata ttgaagtgac ctatccggac ggccgtgttg
     1861 ccatgccgat ctccgaggga cagcaccagt gcgaactgat gctggctctg gttttcccgg
     1921 attaccagac tggctgggac gcttccaacg actattccaa cacggacttg ctgaaaaacg
     1981 tgggcgagta tgtcatttcg gactgcattc cggtatacca gaacggcgtg ctgatctccg
     2041 gcagagaacc ggacggcaag gagccggtga caactactgt taccacgccc cagaagccgg
     2101 atacgctgct gggcgatgtc aatacagaca gcaaggtgaa cactgctgat ctggtgctgc
     2161 tgatccagta tctgctgggc aacaagaagc tttccaaggt cggagctgcc aacgccgatc
     2221 tccacgccga caagacggtg aacggtctgg acgcagcagt gctgcggcag aatctggcgg
     2281 gggattgaaa gtcttttttg taatgaaaaa gattccacgt gggaacccct ccaccgccta
     2341 acggcggtcc ccctcctcta aaggggaggc ttggcttgtt caggctctcc tttgttgtat
     2401 tataggctcc cctttagggg agctggcagc cgtaggctga ctgagggatt tcttccgtaa
     2461 aacctaacac gtaaagcaaa agattccaca agaacccctc caccgcctgt cggcggtccc
     2521 cctccccttt caggggaggc tgatttatat gcgggattgg ttgccctagt tcttctcgtc
     2581 cagaaagaag tctacctgtt gtttatagtt gagccactct ttccggtact gttccagcag
     2641 tgccctgccg gaaactgtga tgcggtagta ccggcggttt cgcccttgat aggggcggtc
     2701 ataggtggta aggctgccgt tcttttgcag ccgccgcagc accggataca gggtggattc
     2761 tgaaacggca aagtgttcct gaatgtcctt ggtgatctga tagccgtagg tgtcctcctt
     2821 ggtcagcaga gccagcacca ttgcatccag cagcgttgca ccaattgaaa atgcagacaa
     2881 tggtttcacc tcctcagggt gggttttagg caataccgca caaagattgt gcgacagatg
     2941 cgtcgtcaaa ttttttgtga aatatgacgg aaaatttgca agcagatggc gtgcaaagtc
     3001 gccagacgct ctttgtgcgg tattgccttt attgcaggaa aattcgaaga attcccaaaa
     3061 taagcagatg aacaggataa aaaagataga aaaaccattt ggaaaatgtc cggcatcgtg
     3121 ttccgcgtgc tccattatag cacaacaaaa tgccgagggc agatgtgcca attcccgcca
     3181 tacttaaaaa tgcatacagc aggttttctt ctaaggagaa acgtcccaat ccacccagta
     3241 cattcattcc gccaaacagc agtgctgcaa cagcaaatgc aatggcttct ttggtgcggt
     3301 tctttcctgc ccagcgaaac agcagtgtga aaatcggtgc cagccatgcc cagtcgctga
     3361 tgctgcttag caggatcagt gccaaaaccg caacgccctt tatccagcga ttgtttccgt
     3421 gttccagcac ccaaaggatt ccaaagcaaa tacagagggt aaacagcatg ttcaacccgc
     3481 agaacctcag cgtatctgtt tcagaaaatg ccaagtcata cggcagctgg gagattcccg
     3541 caaacaacag caagcggaaa aagtagtttc gcttcgaacg ggtgtagcca tatccctcca
     3601 ccaaaaaata gaccatggaa atcgctgtga agtatcccac tgcggtgaac agttcacaca
     3661 gcaccgtccc agcgggcaga aaaattgtag cgatgtgatt gagcagcatc gtccccatag
     3721 caatgtattt taggacgttt tggctccagc ccgcatgtaa ttttgtgtca gactgataca
     3781 tcagataccg ccttcctctc tctttgtgga atgtgcgttt ctttttttat tgcaatatta
     3841 tacgtcgtat aatattgatt acgcatatat tatagcacgg tgaagcctat ctgtcaagcc
     3901 aaaacatggt aaaaatggct tcgaatgtaa acaatcataa gacatcgtgt aaattcgagt
     3961 tcatagttgc acgtcggcgt gcaacttttg ccctctgaga gcctattagt gagaggggtt
     4021 gtcggaaacg aacagttcct gtcgcagaaa accgaaagga gaaatggaaa gatatgtcaa
     4081 actcaacgga atccaaacgg cgtgccctgt tctgctgtca ctatgccaga ctgggcgatg
     4141 tcgcagaggc ggcacggtgt gccggatacc caccggaaac ggctgcggca gacggggcgg
     4201 cgattctccg gcagcagtgt tatcgccgca tggtggcgaa ctaccgcagt gtgctccacg
     4261 atgatccggc ggcagcggta cgggcaggac tggaacggct ggcgttcggc agaagcaatg
     4321 atgcgatcgg gctgctgctg gcagaggaac cgcctggggc ggcagcggta caggctctgg
     4381 atctgtttcc ggtgtcctct gtgaaacggg acaaaaacgg cggcatggag atccacttct
     4441 ttgaccggct gaaagcgttg acggccctgt atgagtacgg cggcgatgca gacggcaagg
     4501 cggcggcaca tgccctgctg acggcacttt ccggcagaga ggcggtggac agcgatgcag
     4561 aagattaaga acttttcgcc ccagcagaaa ctcgccatgt cgtggtggag ccgcaagccc
     4621 tactgcgact atgatgccat tatctgtgac ggtgctgttc gttcgggaaa aactctcgcc
     4681 atgtctatcg gctttgtcag ctgggcgatg acctgtttcc agaacggcag ctttgccctg
     4741 tgcggcaaga cgatcacgtc cctgaagcga aatgtcatga caccgctgct ggaactgctg
     4801 ggcagtctgg ggttcacctg tctggaacgg gtgagcagga attatgtgga catttctctt
     4861 tccggcagga cgaaccggtt ctatctcttt ggcggcaggg acgaaagttc tgcggcactg
     4921 attcagggca tgacgctctg cggggtattt ctggacgaag tggcactgat gccccgttcc
     4981 tttgtggagc aggcactggc acggtgcagt gtgacggggt cgcggctgtg gttcaactgc
     5041 aaccccgacc acccgaacca ctggttctat cgggagtgga tccgcaagac caaggaaaaa
     5101 cacgccctgt atctccattt caccatggcg gacaacccct ctctttccaa gcgtgtgcgg
     5161 cagcggtacg aacgcatgta cagcggggca ttttatgacc ggtttgtact gggcaagtgg
     5221 acggcggcgg acgggctggt gtatcccatg tttacaccgg aacgccatgt ggtgccgtcg
     5281 gctccgccct gtgaccgcta ctggatctcc tgtgattacg gcacagtgaa cccgtcctct
     5341 ttcgggctgt gggggcactg tgacggcacg tggtaccggc tggaggaata ctattatgac
     5401 gcaagagccg agggcgaacg gcgaaccgat gaggaacact atgccgcatt ggaacagctt
     5461 gccgccggat atgacatcga aactgtggtg gtggaccctt ccgcagcaag ctttatcgct
     5521 tgcattcaca gccacgggaa atttcgggtg cttccggcag acaacgatgt gaacgccggc
     5581 attcagcagg tcagccgcct gctgttgcaa gacaagctgc ggttctgcga atcctgtcgg
     5641 gatattcggc gagagttcag ccagtactgc tggaacgatt ccattcacgg agatgctccc
     5701 aaaaaggagc atgaccacgc catggacgac atgcgttatt ttgtacgcac agtggtctgc
     5761 cggaatccgg cagacggatt ttttgcggta tccgcagcaa ggaggtgaga tgacgggaaa
     5821 agcactccgg caataccgca caaagagcgt ctggtgactt tgcacgccat ctgcttgcaa
     5881 atttcccg
//
LOCUS       DAWBGR010000162         5884 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3396_length_5884_cov_3.080631, whole genome shotgun sequence.
ACCESSION   DAWBGR010000162 DAWBGR010000000
VERSION     DAWBGR010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3396_length_5884_cov_3.080631"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1521 a         1388 c         1401 g         1574 t
ORIGIN      
        1 caatggattt ctaaaaccag aaacggcggt tccggaactg cactggtcaa aaatatcctg
       61 caatcccgtg agtatcagaa caaaagcaag aatgccagtg atgcagatta tattgcagac
      121 ctctatcagg cgtttttcgg ccgcagctgc aatacttctg aggtgcaatc gtggaaaaat
      181 gttttgagca acggtgtttc ccgcaattat ctgatggcac agtttgcatc ttcggcagaa
      241 ttcaaaaaga cttgttctgc gggcggtatt tccagcggaa atattacact gactgaagaa
      301 cgggacaagc accccggcgt ggcaaaaatg gttgctggct gttatcagat tctgggaaga
      361 acgcctgcgg ggacagaagt ggaaaactgg gtcaaaaaga cgatcactac cggttctggt
      421 gcggaactgg cagacggatt cttcaaatcg caggaatatc acaacaaaaa taccagtaat
      481 gcacagtatg tcaacgacct ttatacggca attttcggaa gaactgccga cagcagaggc
      541 ttttcttcgt ggaaaaacgc tttggacaat ggtacttccc gcgatacagt tcgaaatgcc
      601 ttctatgaga gtgctgagtt caaacagctt tgcaaaaaga atggtattgt agacaagaaa
      661 aatcgctatc cgaaagctgc ggctgttctg aatcaggtag gctgggatct gaaagcggcg
      721 ttccagtggt ctgccggcat gaagtattcc aagtacacgg cgacagctgc tccgggaacg
      781 gaatattacg caaattatgg attcacctgc aaaaccggaa attgctatgt tatggcagct
      841 acattctgcg aaatggcacg ggaactcggc tatgacgcaa aacagatttc cggcagcgtt
      901 ccgttgagaa gcggcggata tggtccgcac tcttgggtgg aaattgaaat caacggaact
      961 acttatgtat ttgaccccga ctttaccaat gaaaccaaac gaaatggata ccagatcaca
     1021 tatggacagt ctggtacatg gagatacaat cgtggttctg taatgaactg agaaaacaca
     1081 acttgggaat ggagaagaaa aatgaagaaa acttacatga catggcttgc cgcatttttg
     1141 gcagcgggtg tatttttgac tgggtgcagt caggagaagt cgtcttccag tgaatctgca
     1201 actgaagaaa ctacagcagc aacggtcgct actgctacca cagaagaaac tgaaccggct
     1261 accgaagccc cgacagaacc ggaactgaaa acgattggcg aaaaggcaga aggcgaacac
     1321 gtttacaacg tgacgctgaa aaatgcaacc ggaaaggaaa tctccggcgt ggcgatcaag
     1381 gacagttctc aaagcgaatt ccccgaaaac atgatgaaag acagtgatgt ttttgccgca
     1441 gatgaagaac gtgttcttta ctatgatgca acagaggcaa tcgctgccaa cgctgcggaa
     1501 gaaacagatt ccgacaagat tttgactccg gaatacacga tccagctgac attctctgac
     1561 ggtactacag cagagctgca tcagttcccg tttgatgatg tagaagctgc ggagatccac
     1621 tgggaagatg aaattgctta tctggtatac acttctgttt cctctcaaac cgaggtaaat
     1681 acaaaggaag cagaacagat gcaggtacag acgagtgcag aaacagagcc ggctgcacaa
     1741 gatgcagaag aagtggcagc tgaggacggc tatcaggaac agcagcccgt ctatgtagaa
     1801 ccggaagaat cttatgacga aacaccggca gctgctccgg tagaaaatac accggcggac
     1861 gaaacaccgg caaacaataa tgatgctgcg gatccgaata atggctgcct tggagatgag
     1921 ggattattta actaatggaa atcacaaaac gacgtgaaat tttctttctc tcggaattgc
     1981 gtgcaattgc ctgcattgcc attgtcatac tgcatacgtt ctatgccgcc agtgtttacg
     2041 caccggatca tactgcaaaa attgccgctt tgacgacgag aaatctcatg atgtgggctg
     2101 ttccctgttt tgtcatggtc agcggtgcac tgcttctgga ttcagcacgg gaagtgacat
     2161 atcggaagat attccgaaag tatatcccgc gagtgctggc tgcactggta ctgttctccg
     2221 cagtctttca gatttttgac gatgctaccg gcgaaagcgg cattcagttc aaaacgattc
     2281 tttatgggtt gcaaaatgca gtgaccggaa gcggctggaa gcacatgtgg tacttgtatc
     2341 tgattacagc attgtatttg ctgctgccgt tttatcggaa aattgctgct tccctcaact
     2401 caaaagatac gtactacttg ttaagcgttt atctggtgtt tctatcgctg ctgccaatgt
     2461 tgcaaacact aacagaaaaa tcgacagcat tctatatttg tgtttatagc gtctatcccc
     2521 tttatctttt cttgggtgat gcttttatgc aaaaacgcat ttgcaaaaag ccgtggctga
     2581 tgattcttct ggcttctgtg ggaacaattg cagttgcctg tctgacaatt cttagtgaga
     2641 aaaagtccat cgccgcattg tcagaacttc ttaccagtta tgcattcccg ttgatcgtat
     2701 tgcagtctgc cggaattttc gggctgatgt cctacagaga aaagaaagca cctaaagtgc
     2761 tctattggat tttacatcag atcgatcagt gttcgttcgg tgtttatttg atacacatgc
     2821 tgtttctgaa actggtgctt gtggtctgga aatggaatcc ctatatgcat ggcggatttt
     2881 ttatgatcct ggcactttcc gttggaacgt ttctctgctc gttcctgtgt attcggttgc
     2941 tgaaatatgt tccaatactg aaaaaaattc tttaaggcaa taccgtacaa agtcgccaga
     3001 cgctctttgt gcggtattgc cttaagtttg aaaaaacgat ttgattatca aaaaagagta
     3061 tctcaaatga gatactcttt ttgtttgtca ctttctattt tggcaatact acttttgcaa
     3121 aaacacatta ccgcattgca cgaatcagcc ggaacacctg ctctgctgta tcagtcagat
     3181 tttgccttgc agtttctctg tatagagcgt tctccaatgt ggttatttct cgcagtatcg
     3241 ggaaaaaggc atcgattccg tgaacattgc aaatcgtcgc tgcatgttct gtgcagccgc
     3301 aaaatgccag cactggtttc tgatattgct tggcaagagc agcaaccccg ataggtgctt
     3361 ttcccatgat gctttgtgcg tccagtttcc cctcgccagt gataacaagg tctgcattga
     3421 caattgcctg ttccagttga gtttcccgca taacgatctg aatgccggat tccagttcag
     3481 cgtgcaggta gtttttcaac gcaaatccca gtccgcctgc ggctccgctg ccggaacagt
     3541 tggggtcact ttccggaaac agcatttttg tcagcttggc atatcgggaa agccattcgt
     3601 ccatttgggg aatcattgct gcatcagctc ccttttgcgg ggcaaagacg gcactacagc
     3661 cctgttcccc gcagagtgga ttggtgacat cacatgccac ctgaaatgtg caagccgata
     3721 attccggcag tacattttcg gcggtgattt tttgtaactg cgaaagtccg actgctccgc
     3781 agggaatctc ggtgttatct gcgtttcgaa aagaaaagcc cagtgcctgc aaacagccta
     3841 cgccgccgtc attggtcgca ctgccaccaa taccgatgat gaattttcga cagcccttgg
     3901 caatggcatc tgcgatcatt tctcccacgc caaaagtcgt ggtgtaaagc ggattccttt
     3961 cggattcgga caacagcgga agtcctgccg cagctgccat ttccattacg gcggtaccag
     4021 acggcaaaat gccatagttt gctgtttgct ttctgcccag cgggtcggat actgttactg
     4081 tttccaaata tccggacaac ccattaacca aggcttctgt agtcccctct ccgccgtctg
     4141 caattggtga aacctggatc tcagctggca tgccggtccg tcggatccca tcggcaatag
     4201 catttcctgc ttccagagag gataaactgc ccttgaaaga atctgtcgca atcagaattt
     4261 tcatgcttct tgtgtctcct ttcggagtgc ctgcttcata ctgcacagat ccagtccatt
     4321 cagctgaccg tctgcattgt aatctccggc agtccaatca gtcacattgc cgctgcctaa
     4381 cagccatttc tgcaccataa caacatctgc aatggtgaac tgtccgtctg cgttgacatc
     4441 tccgatcact ttatgcgaat tttcctcaag ctgcaatgca tcaacacgct gtatcatgtc
     4501 tgattcccga tcataggtaa actgtattga aattttgtcg ccagcttcgt agtggctaag
     4561 atttttgctt tcgtccccgt gatagtagga gtccccttgg aagaaaaatg tgccgtattc
     4621 tgcaaatgtg acagaagtat tcggtactat ttcagaaatg gtgtctgtga acgatacggt
     4681 ttcataagcc ggctgtgttt ctgaaaccgt tgttgtaacc gtagttgttg tggtttctgt
     4741 tgtttctgca gtagtttctg aagttgtggc agttgtggtt gtagatggtg ccgtaagatt
     4801 gaatctaccg tcctctttcg ccagatatga ccagagatac cggaaaaacc aaccgtcaaa
     4861 gttagtgatc gtttgagaag aacctgccat catgatcgac gtgccgccgt ccggaaatcc
     4921 tcccggcgga aagccgtcgg attctccctc gccgccataa aaatcagtca gtccgaagct
     4981 gtgtcccatt tcgtgttcta gaatatgcat actggaagtg ccggcgatca agcccaaaat
     5041 ggcgttgtct gaaagctgct gtccccagtg gtagccgaag ccgcccatat cgatcatgcc
     5101 ttgcgttgcc tgcaagtaca tatcgtaacg attttcgtag ccgtctggat actggtatga
     5161 ctgatctgcc cagtgaatat agcggtatcg tgtttcgtcg ccgattggtt ccaatgtagg
     5221 cagctccgaa accgccatgt ttccgtctgt gatgtagctg gtatctgctg gaatcgtggt
     5281 ggtatacacg gtttcgtccg gctgcagatc cagcaggcag ttctgatcca gcactgccca
     5341 gccgacaatt tgcacattga catgggtgta tggccagttt tcataaccta ccagccagtc
     5401 agtccagtcg ttcactgccg tttccagaac ggtttgcagc tgcttccgct gctccagtgt
     5461 gattgtctgt ttgctctgcc attttacaac atacttgagt gttccgtttc cggcgacgat
     5521 ctggtcgtaa atcgttgccc agtctttcac agagccctct gaaatgatac gattttgcag
     5581 aatccactcc gccgcagatt gatatgcact gggcatatct gccagcgttg taccggaaga
     5641 tgcagcctgc actgcaaccg gcgttacggt attcggaacg gatatgggtg caaccagagc
     5701 acaaacacta agtacggctg ccgccgcggc ggaaagccat ttctttttca tacaattcct
     5761 cctgttctga tttccaatcg aaaacatctt cttttattat gccatatgtc gaaataaatg
     5821 tcaatataca tagggcaata ccgcacaaag agcgtctgac gactttgcac gccatctgct
     5881 tgca
//
LOCUS       DAWBGR010000163         5713 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3518_length_5713_cov_3.862496, whole genome shotgun sequence.
ACCESSION   DAWBGR010000163 DAWBGR010000000
VERSION     DAWBGR010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3518_length_5713_cov_3.862496"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1336 a         1444 c         1670 g         1263 t
ORIGIN      
        1 tgcaagcaga ttgcgtgcaa agccgtcagg cgggctttat gcagtgtttc ctttattata
       61 acatatgttt ccggagattg caactgcttt ttgcggcggc gatttgtcct ttcacaaaaa
      121 ccctctgtac attcccgcaa aaatgtgcta tactatatac attaaggcaa caccgcacaa
      181 agcccgcttg acagctttgc acggtgttgc cttagaccaa tgtgaaacag ggaggattac
      241 atgaaactac ttgccattga cggaaacagt atcctgaacc gtgcattcta cgggattcgt
      301 ccgctcagca atacacaggg cgtgtttacc catgccattt tcggctttat gaatatttac
      361 ctgaaaaatc gggacgaggt gcagccagat gctgtggctg tggcatttga cctgcgaaag
      421 cccacgttcc gtcacaaggc agttgccacc tataaggcga accgcaaggg catgcccgaa
      481 gaacttgcca tgcagctgcc ctatgtgaag cagctgctga cactgctggg gattccggtc
      541 atcacctgtg agggttacga agcagacgac attctgggaa cgctggcggc ggcatgtacg
      601 gcacaagatg cggactgtgt gatcctgaca ggggacaggg acagcctgca gctggtcagc
      661 gatcatgtga cagtgcggct tactaccaac aaggaaacca ttccctatac gccggagcgt
      721 tttcaggagg agtacggttt tgctccactg tccctgatcg acctgaaagc tctcatgggg
      781 gacagttcgg acaacatctc cggtgtagcc ggcgtaggac agaagactgc ttccaagctg
      841 attcaggcgt ggggaactgt ggaaaatctc tatgctcatc tggacgaggc agggctcacc
      901 aagtcggtgt acaataagct gactgccgga caggacgcgg caaaggagag caagtggctg
      961 gcaacgatcg tgaccgatgt gccgatcgac atggacgtgc agcactatct gccccagccg
     1021 gtgcagaccg aggaagtccg caggctgctg ctggaactgg aaatggcaaa actgctggaa
     1081 aagctgaagc tttccggaac agatgctccc gcaccagaaa cggcagagct ggcagaacag
     1141 cagcagcctc tgacggaaca gcccttgacg gcggctctgc tggacacatg ggctgctgac
     1201 aaaatggaac tgccctgctg tctgtttgac ggcagccgca tctgcgtata ccacgccggc
     1261 acgccggaag cggtgtacac ttcgacagat actgccctgt gggaacagct gtttcagatg
     1321 ccgcgtctga cgttcggggc aaagccccag tatcggtttc tgctggcaca gggcatcacg
     1381 ccggacagcg gcagccctgc ggcaaaagat gcggaactgg ctgcgtatct gctgaactcc
     1441 accacgtccg cctatgaggt cgaacggctc tgcgtgacct atcatgtgcc ctatccccgc
     1501 agtgcaggtg aatatgccga tctggcggct ctcccgaagc tgtacgccgc tatggagaag
     1561 ctggtggagg aacagggcat gacccgtctg ctgcactttg aacaacagct gaccgtggtg
     1621 ctggcggcaa tggaacacga cggcattgcc attgaccggc agggcgtaga ggacttcggc
     1681 gtgtacttgt cggaacagat cgaggaaatg cagcaaatgg tctacgacga agccggacac
     1741 actttcaaca tcggttcccc gaagcagctg ggagaagtgc tgttcgggga aatgcagctg
     1801 ccccacggca aaaagaccaa gaccggctat tccaccaatg cggacatttt ggaaaaactg
     1861 cggggagagt atgccattgt ggactatgtg ctgaaatggc ggcagtatgc caagctgaaa
     1921 tccacctatg tggacggatt gctgaaaacc gttggggcag acggcagaat ccacacggtg
     1981 tacaaacaga ccgaaacccg tacagggcga atctcctcca cagagccgaa tctccagaac
     2041 attcctgtcc gcacagaact ggggcggaat atgcgaaaat tcttcgtggc acgggagggc
     2101 tgtgtgctgc tggacgcaga ctacagccag attgaactgc ggctgctggc aaacctgagc
     2161 ggcgaccggc agatgcagga ggcattcctt agcggggcag atattcacgc agccacggcg
     2221 gcacaggtat ttggcatgcc gcgggaaatg gtgacaccgg agatgcggag tgcggcgaaa
     2281 gcggtaaact tcggcattct ctatggcatg ggagcctttt ccctgtcgca ggacattcac
     2341 gtttccgtgc ggcaggcgaa ccagtatatc cagaactata tggcacagtt ccccagcgtg
     2401 ggggcattca tggaaaagac cgtggacact gcccgcatca acggctatgt gaccacctat
     2461 ttccagcgac gccgaccggt gccggaactg ctcgccagca acaaaatggt acaggcggca
     2521 ggaaaacgaa tcgccatgaa cacgcccatt caggggacgg cggcggacat tatcaagctt
     2581 gccatgatct acgtgtaccg gcggctgcaa agcgacgtgc cggacgcgaa actgatcttg
     2641 caggtacacg atgaactgat cgtcgaggta ccggaacagc atgccttaca ggcgggaaaa
     2701 gtgctgggcg aggaaatgca gaacgtgatc cggaataccc tttcgccgga ggagcaggag
     2761 aaattccctg tgcccctgac agcagatgtg agcatggggg agagttggta tgcggcaaaa
     2821 ggttgagatc gcaccggcgg cagaagccga tctgccggag attctgtcgc tggcgaaagc
     2881 agcatttccc gatccgtgga cagaacagca gttccgccag accatgtgct gttgttacag
     2941 ccgtatctgg tgtgccaggg cggcagggga attctgcggc tatgtggcag tttcgctggc
     3001 aggagatgcg gtgaatctgg acgacatcgc agttgtaccg gcgttccggc ggcagggcat
     3061 cggcagacag cttctggact gggcacaccg gcagtttccg gaaaacgaat tctggctgga
     3121 agtgcgggag tccaacgccg ccgctattgc cctgtatgaa tccgcaggct atcggcaggt
     3181 gggctttcgg aaacggtatt accacgatcc ggaggagggt gctgtgttga tgacgcgaga
     3241 ggtgaaaaaa acatgacgga actacgaatg caactgaaag cttttctgct ttggacattg
     3301 ttgcttgtac ctccgtttgc gttgactgca ctgaatttgt ggaacctttt tgcggagtgc
     3361 agcggcagga gaaaactggc tcccagactg tttgccggac tttccatggg aattggtgcg
     3421 gcagagtatt ttcttctgtt gtgtttttgg tttgaatata gcggaaagga ttatgaccag
     3481 gcgacatatg cttttcagac acataatctg atcgactcgg cacatgcact ttctttctgg
     3541 ctgccggttt tggtgggagt cgtcgggttg gtgcttttgc tctggtgcag accggaacag
     3601 ctgccgccgc tggtttctgc cggtgcagtg gcaggggtgc tggtgggaaa tatcatgcag
     3661 atacttctgg cagtgcagat tgccgaacag gtagatcttc acaggtttct ggattggtat
     3721 ttctaccttt atcacggcaa tctgctgctg ttgtctgcga tggggatccg ccggcatatc
     3781 gccggacaga caaggctcct gcggaaacgg cagacggtat tccggcggcg ttgggtgctt
     3841 cggctgtatc agtttttaga aaagacgtca cattttcggc tggctgcctt tttgctggtg
     3901 ctgccccttg cggcagtgct ggaaatagtg ctgattcttt gcggacaagg tgcagacggc
     3961 acagttcagg cgtttaccca gacggcggac tggacatttt cacagcagct tccgccgcct
     4021 cctttggaat ataagggaca ctatctctgt acagttgccg ccggcggaca ccggaaactg
     4081 gtgcgtccgc ttcggctggg gtatcgccgt ggtgccgtga tcgtggtaaa ccgacagctt
     4141 gccgtggcga atgcctttga agaactgctt caggaacgtc tgccgcatct gcaccggctg
     4201 gtacggcatt tctacgataa tcacggctat ccgctttcgc aaaaaatcac tacgccgttt
     4261 cgggcggatc tggtgtattt gctgatgaag ccattggaat ggctgtttgc agcggttttg
     4321 tatttgttcg atcttgcacc ggaatgccgt attgcaaggc agtataccgc aggaaagcct
     4381 gtgaaaaatt ggagtgaaaa aacatatgct gatattagga attgaaagct cctgcgatga
     4441 aaccgcagcg agcgttgcca gaaaccacac ggagattctc tccaatgtgg tttcctcaca
     4501 ggtagaggaa cacaaacaat acggcggcgt tgtgccggag atcgcctccc gcaggcactg
     4561 tgaaaacgtg ctgcctgtgg tacagaaagc actggacgat gccggtgtga cattggcgga
     4621 actggacggc attgccgtga ccgcataccc cggactgatc ggggcactgc tggtgggcgt
     4681 gaacttcgcc aagggactgg cactgaccgc cggaaagccc cttgtgccga cgcatcatct
     4741 ggcagggcac attgcggcga attacattgc acatccggaa ctgaagccgc cctatctctg
     4801 tctggtcgtc agcggcgggc acagtcatct ggtggaagtg ctgacctata cgaagttccg
     4861 gattctggga cggacacggg acgatgccgc aggggagtgc ttcgacaagt gtgcccgtgc
     4921 tatgggctat ccgtatcccg gcggcattca tctggacaaa gcgtcccagt ccggcaaccc
     4981 gaaagcattt tctctgccaa aaccgaaaat tgcggaaaat gcctatgatt tcagcttttc
     5041 cggcttaaaa actgccgtgg tgaacctgct gcacaatgcc cagcagaaag gcgaaaccat
     5101 ttgtcgggaa gatctggcag cttcggtgca ggcgactatt tccgaggtgc tgacagaaca
     5161 caccattcgg gcggcagtgg agcacggcta ttccaccatt gccattgccg gcggcgtttc
     5221 tgccaacagc ggcgtgcggg aatctctgca aaaagcctgt gacaagcgga acttcaccct
     5281 gtacatgccg ccgctttccc tgtgcggcga caacggagcc atgatcgcct gtgccggttc
     5341 ctatgccttt gatgcaggga tccgtgccga ttccagcctg aacgccagtg ccactatgga
     5401 actgaccgac tggggcgata tgggaaatgg gcggtgatgc ctaagtttta caaagcagca
     5461 aaaagaatcg ttcgtatttt cctcgcaatt gtatcatttg tcattctttc gtcatgtgat
     5521 atgcatgacg atgtaaaacc aagaaatcaa gatgatacaa ggtggattac agctgatcaa
     5581 aatatatttt tttctgttat gtacgatgga aactataata tttgtttagg tgctttaaaa
     5641 acgactgaaa cagtctatca tattagtctt gaatttgatt atggctccgg acttatcatt
     5701 agtgattatg ata
//
LOCUS       DAWBGR010000164         5618 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3578_length_5618_cov_6.469890, whole genome shotgun sequence.
ACCESSION   DAWBGR010000164 DAWBGR010000000
VERSION     DAWBGR010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3578_length_5618_cov_6.469890"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5298..5307
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1627 a         1198 c         1041 g         1742 t
ORIGIN      
        1 gaaaattttt tctgtttttt taattttagg ggttgacttt tatttgcagg tctttgcata
       61 tttttgacga taggtctgag aactaaattc cgctaatatt tggcgaatca cttctgcttg
      121 ccgaatatct gcaaacaata gcaattgctc caaagcaaca gattgttctt ctgtaagcat
      181 atttttatat ggatgatacc aatctgctac tctaacaccg ccgccattcc cggaacacgt
      241 ctccagagga tagtccaatg caagtgcatg aatgtcgttg cgaacagtat tacaacatac
      301 acacaattcc tgtgcaagaa gtggaactgt tgtttgtctg cgagcaacta aaatcttcat
      361 gatttcggca cgccgttcat tcacactcac atggacaccc cctttcctaa tcgtcgaact
      421 tactctatca ttcaaactac cagccttttt ggtagtttga aaagagttca caaaacattt
      481 tcaatttgta caggcataaa aacaccgaca aggtacagaa aaaaattctg cacctcatcg
      541 gatgttctca cttttttacc aaactggtca gccatggagc aatgggtctt gcaatcattc
      601 tcgcattcag atatgccatt tccagtgtca gacaagtact gcccagataa tatccgtttc
      661 gttctgtcaa ggtcatggca aggttcggtt tttccatatc tgttaaacag atcggcagca
      721 gaaactgcaa ttgattctgg tatccctgcg gtactaccag ccccggctca attactgctt
      781 ttcgtctgcc cagttccact gctgtttcca gcagcaatga cagattcttg aaccgaagca
      841 gcttctttgg cagccgttct cgattttccg ggtcgctgag aatatgttct gcatttaccc
      901 gaattggcca ttccggattg aagtttacac cattttgcat catcgggaaa tatggctttt
      961 tgggcagcgg ctctacatat cgcagcttgg aagaaacagc atcacagaag ccggtgaaat
     1021 accatttcaa tgtggtgtct ttctttttat ttcgttcaaa gcaggcgtag attgcctgat
     1081 actgccttgt gtacagtcct gtatgaaagc aggcacagtt gttttccaca tggaaatacg
     1141 ccgtttctcc ggtgttgtaa tcgatgctca gtttccggaa catcatatgg agataccgtt
     1201 ccaaaatcgg cgtatctgta tttttacatt cggtctgcgg ttttcggaat cgccatgctt
     1261 ccggtaacgc catttctgcc aattgttcta actgcccata ccaatccggc acataggcaa
     1321 attcaaataa atctgtttct atcattttat caccttacac ttagaaagtg cctccagata
     1381 ctttgtttgt atctctgctt tctgatcttc acacacatac tggtgttctg tgccgtctga
     1441 acaaatctca accacagttg acaatgcact gatatatgca tcgtaatact gcagcagctt
     1501 ttcctgtgca tcatggtccc ctttgacagc acgatacacc gtttcataat ccagtttcat
     1561 gcttttgtgt cctttcgtaa taactacgaa tatttgcaaa tgcgtttttc ttccgttctg
     1621 acacggagcg tctggagatc cgcaatgttt cggaaatctc ggaaatagac attccatacc
     1681 agaattctaa aataaccact tccttttgtt tgggcggcaa cagcagcatt gcctgataca
     1741 gccattccgt tgcaacgaca cagcagtatc cgtttccggc atcgatgaaa tgttcggatg
     1801 gatagactgc attttcaccg tgtgttttca aaatgtactg tacgatatct gtgttgaccg
     1861 actcattttt ccttcttgtt tttgcagcag cagcgaaatt tctgcttgca tttctcatca
     1921 ctgttttact gaaactatcg aaaatctcaa gcttcagctg atcatcatgt gagggagcag
     1981 acattatttt ccctccctcc atattcagtt ttgagaagcc cttttttgcc ctcctgaaat
     2041 tactaagaca attgagagaa gcaaaatggg aaggttttag atgtaaattt tatatgaaca
     2101 gacaggagaa agaaaaaagc agcatacaaa accggtcatt tcgccggatt gtatgctgct
     2161 tgaggacaaa aaaaaggggc aagtccaaca gtgttaatgt tgaacttgcc ccgaaaaatg
     2221 aaaggaatgg agaatcatta gaacggacaa ttttcatgaa aagatgcaag catacagatg
     2281 tgaaagggaa gtgctggaat cgattctgcg tggaaaagtt tattttaatg ataggttcca
     2341 tctgatgctg catccttccg gcgtaaaaat acaccataag cggattttag gatgattgga
     2401 acagtttcat ccaaattgga acagctattt tgagaagcac aaattcgcct agatgctgca
     2461 tcatttcaaa ccggaaggat acagcatcta cattttgtag atgccgtatg cttaattgta
     2521 ttgttttgaa atttccggct tttaccgttt ggagtcctct tcctgtttta acaaattata
     2581 acgcatctgg caaaggtcaa atacattcaa agtttgatct ccattgaaat ctgccgcttt
     2641 ccagtctgca agctgtgtgt ctggaacaga caacagccat ttttgcagca gtacaacatc
     2701 agaaattgta aatgcaccgt ctgcattaac atccccaatg attcgctcct gatactgaat
     2761 agtggctcct gttagctttt cattcagcag accaatggta atctgattcc actcttcttc
     2821 cgttccgtta taggaaactg cacgcagatt tttgcagtta taaaatgcgg aatctcccac
     2881 cttaatcagg gaattcggaa ttgtaagggt ctccagataa ggattctcgg aaaaagcata
     2941 cattgcaaca gatgtaacac cgcttggaat gatatatgca gcatctgtct ttgctgccgg
     3001 atagattatg agtgtatctt gcttcttgga atacagaact ccatttagac tagaaaaatt
     3061 gggattatct ttcgcaacct gaatctcctg caatgcactg caagagccaa acgcattttt
     3121 atcaattttt gcaactccag ttgaaagttt cacagacttc aaagaagtac agcccaaaaa
     3181 cgccatttca ccaatggaag ttactgtatt tgggatttca atagatgtaa ttgcagagag
     3241 ataaaatgct ccgacagaaa ttgcagtaac tggaagtcct tcgatctcag agggaattac
     3301 aacatcagaa atggaattgt caaagccggt gatctcaatt gaatctccat ggttgacata
     3361 ggtgaatgcc gatgtagctg ttgcaatacc agtagatact tgatcagggt ctttgacagc
     3421 aatgtccaga taacacgtca catcagaaaa tccaccgacg ccctcacact gataggcata
     3481 ccaatgcttt tccgcatcat agttcacaga gttttctttt acatatccct ctgcaatcag
     3541 ctgatcaaac agtgcgtccg ccatttgctt tctggtttcc tgatctgcat tctgaaatgt
     3601 ggtatcgctg gacagtgcgc ctaccttttg tgcgatttct gccattcttt cggcatcagc
     3661 atccgatacc gcatcataaa ttacgacaga aaaggttttt gtggtagaaa ctgtcccatc
     3721 agcatctgtc aggcgagcat aataagaata agaaccaact gccgcattct caaacgacag
     3781 cacagcagac caggttctat cattttccag gtcatctcca ctaacgtcga agttgccgtc
     3841 atccagcatt tttgcgacta cttcgtttgt tttggcatca accagttcta catcgcttcc
     3901 ggaatacttt aagcaagtag ccgtaaagac caccttttta tcggtttcat cctccataaa
     3961 ctctgcgttt ccagtatgct gatttaaccg caatgtaaaa gaaggatact ccgctgcaca
     4021 aatttgctcc gcaccgacaa aaaagcaggt gccgctcatc attgcagcag tgagcagact
     4081 cagaactttt ttcttcataa aaacttcctc ctcattaaaa ttatatttat gataccataa
     4141 aaaaagtacc tgtgcgagaa gtctacgaaa cttacagtgt tttcgacgaa acttacagtt
     4201 tttcatattt tgtttttatt ttgcagccat gcttttttac aaatacagag ccacctcagc
     4261 attaaaatag acatcattat gatggagttg acagattcca tcatattttt cagctgctga
     4321 ttctacattt ttcaatccat atccatgtga ttctgtatct tcttttgttg ttacgatttc
     4381 gccattttca taatgaacct ttcctggata ggtatttttt acagcaataa agagcatacc
     4441 ctttcgataa gtcattgcga ttttcaactc acgttttccg tcagtttttt gaagtgcctg
     4501 tactgcattg tccaacagat ttgtcaaaat tgtggaaagg tctacaattt ctatgggaag
     4561 ttctgttggt ataacaatgt ctacggttac agcaacattc caatcagaaa tagatcttaa
     4621 cttgtaattg ataatgctgt ctaccacaat atttcctgaa gtagagtaca gtacagactt
     4681 tttttcttct ttctgtatca actcttgtag atattgtgaa gcctcgttgg ttttgcgacc
     4741 ttcaagaaaa gcttctacaa tagaaaaatg attggaaatg tcatggtgaa atcttctggt
     4801 gtcttccact gcattctgca tcagctgaca ctggttctgg tagtaatttt tttcttgctc
     4861 tgccactgtt gcttttgcag cccgtgcata tgcaattgac agtgattcat acagaaaaaa
     4921 tactacgata ttgatgataa ataagagaat catggaaact gtgaccgatt gtgctgtcac
     4981 tccagaaaca gtcgtaaaca gcaattctat gccaattgtc agaattggta tgcacggaga
     5041 aacaaacatt gtccatattg gaagaatctt ttgctcattt ctgtgatgcg aaaaatatcc
     5101 taatagcaac acaatccaaa agattaccat acgagataaa aagattccga tctcattttc
     5161 atattcatat ctttgaagtg gttctatata ggcatttttt gtaaccacag atacaattaa
     5221 ttccacggca aatcctaaaa caatatattc taatattgca agcaagcgtt ttggaaaagt
     5281 ggtattatag cataatgnnn nnnnnnntgt aaaaccaaga gcataatgag atagaaaaaa
     5341 gtgagattat cgtgaaaatc agattcaaaa aaggaatgtt tgcaacgaaa tgaatcccaa
     5401 ttgtcaatac aaaatataat gcataaacgc taattgtaat taccttgttt cgtatctgtg
     5461 ttccaaaaag cgattgaaaa aaataataaa taatatagat ggaaaataat tccgaacaag
     5521 cgtatacaat atcgtagttg gataaactca tacggactcc tttcgattga tagcaaattg
     5581 ttctgctctg atctcttttc gttttgactg tgcgattg
//
LOCUS       DAWBGR010000165         5537 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3641_length_5537_cov_5.939073, whole genome shotgun sequence.
ACCESSION   DAWBGR010000165 DAWBGR010000000
VERSION     DAWBGR010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3641_length_5537_cov_5.939073"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1181 a         1494 c         1573 g         1289 t
ORIGIN      
        1 caactaccga tgtcagcggt gcaactaccg atgtaagcgg tgcaaccact gatgtaagcg
       61 gtgcaaccac tgatgtaagc ggcggtacca caactgacgg cagtgaaacc actaccgacg
      121 gcagtggcac tactacagaa actctgccga ccggcgtttc ttctgtagaa gtaaagactg
      181 ttgacgctga aactgctgaa aacatctacc tgtccgatga agaatccttc aacgtggctg
      241 gtctgatcga atccgtaacc ctgcatctgg aaaatggcga agatgttacc gtagatccgg
      301 caactgcaat tgacttcgaa atgactccgg ctgaagctta cgaaactgta acagaaaaga
      361 ctgctggcaa ggtttacttt gacggcgaag ttggtctgaa gtacaagggt gaagaaaaag
      421 ttgagatcac agataccgta aaggtagcag ttgctctgaa gggcgacact acactggacg
      481 gcaaggttga tgcaactgac ctgttcgaaa ctgcttacta tgttgccctg cagggtgctg
      541 gtaagactcc gatctttgat accgtaaaga acggcactgc tctggagagc aagctggctt
      601 atctggcatc tgatattgat acggaatcca aggcaggcga aaatacagaa gatggaaagc
      661 tggatgctac cgacatgctg tacactgcag tatatcaggc atacaacggt gctggtaaac
      721 acattgactg ggcagaagct atcaagatgg ctaacggcgg taagtaagct tttctaagat
      781 tcacaaactt ttatccattt gcttgtatat attagcggtg tgaggtgcat atcgcatcgc
      841 tggtatatca catataattt tatttttgaa aggaaataga aacaatgaag actacattga
      901 aaaagacaat ggcagctctc tctgctgctg ctgttgtagc tgcatctgca gctgttatgg
      961 ctgttccgac aagtgctgct ggcgtaagcc tgactatggg cagcaagacc ctgactctgg
     1021 ctgaactgaa ggctgctaac tatcaggtaa ctctgccgat cggcgttacc gatagcatca
     1081 acagtgttgg tttcggtgta actctgggcg aaggtctgac ttatgttgac ctggaagctt
     1141 ctgtaaagaa ggctacctgc ctggcagcta ctaacgatgc tggtaacttc gtattcgctc
     1201 cgtttgcata tgcaactgtt ccgctgaagg ctgttagcgg tcagatcgca gaaatcaagg
     1261 taaaggttag cgaaagtgct aagccgggcg acaccttcac tctgtctatc gacggtaagg
     1321 attccgacgg cgtagaaacc tcttacaaga acggcgaaac tggcgaaact ggcgttccgg
     1381 gcagcggtgc tggtaccatc accatcgttg aagatccgac cgagccgacc accgaagcta
     1441 ccacagcagc tccgactgct acaccggtag ctgctactac taccactgct aaggttgcta
     1501 ccccgaccaa gccggctaag tccacttctt ctccgaagac tggcgacgct ctgccggtag
     1561 ctggcgtagc tgtagctgta gctgtaatcg gtggcgtagc actggttgct aagaagagaa
     1621 agtaagcatt cgctgcgata gcagcaacat gtgtaactga atctacatgc acataagtgc
     1681 atcgagaggg catctccttc gggaggtgcc ctctttgttt taggcagcac cgcacaagct
     1741 ttgtgcggtg ctgcctttat tgcaaatata cttccccgta cgttggaaac aatcgtactt
     1801 cccgcaggag cagtccagcg gcatgcctac gctgttccaa gctggcatct gcccaccatg
     1861 tttcccaatt ttctgcgggc tgtaacggcg gttcagaggc gttctgacgc agttcctgtt
     1921 ccagccgctg gtgctctgtc cccagtcgct ggagttcttc ccgcagaaat accgttgccg
     1981 catgcggttc ccccagttca gccgccagcc gccgcattcg ctgttccagc ttttccagag
     2041 ccaccgccgc ggcagagggt tccggctctg ccgactgtgc atagggcagc actgccgccg
     2101 cctgttccgc cagcaccgga gccactgcct gttccactgt ttccgtccgc agtccggcaa
     2161 tgccggtgca gatgccctgc cgtttgccgc ggcagacaaa ataggtgtag gctgcccctt
     2221 tgccgttgct gcgggcataa cagcgtgtgc cgcacagccc gcagactgcc agtccctgta
     2281 gccacgaggt gtgtcctgtg ccgcggttgg tgctgccgcc gcgggcattc agccgttcct
     2341 gtacgctccg ccagagcgtg ccgtcgatga agccgcggtg cagtccggcg gcaacatgct
     2401 cgccctccag atgcaccagc ttggaaccct gccgccggct ggggctgccg tagaccagat
     2461 agccgtttcc gcagcagtac gcctccaacg catgtgtcag cactgccccc tgccgctgca
     2521 aaaataccgc acaggacaga tctccctgca catagaccgg acttcgcagc agccggagca
     2581 ccgcactgtt ggaccacctc gccccctgtc gggtacggct gccggcttgg ttggcggcct
     2641 gtaccagctg ctccacgttc tgccctgcca cgccgtatcc ggtgtaaaat gcctgtacct
     2701 gctgtgcctc ctccggctgc acttccagca tggtggtggg gtgccctccg tgtgtcaccg
     2761 gctttgccgt gtaaccgtaa ggcggcacac cgcccagtgc cagctgctgg acggctcggg
     2821 cgtagtaatt gtcccgcacc cgtccggcaa tggttttctg ctccagttcc gcgaacatca
     2881 tcagcagatt cagcagcatg ccgccgattt ccgtctgggt gtcgaagctt tcccgcacgg
     2941 agtgcagggt cacgcctcgg cggcggaatt cctgcatcat tgccgcaaaa tcgcacaggg
     3001 aacgggaaat tctgtccagc ttatagacat agacgctttc gatctgtccc tgttccaccg
     3061 cctccagcat ctgctgcaat gccggtcggc gggtgttggt gccggagaag ccgcggtcgg
     3121 tgtagacctg ctgaggcacc gactgggggc aaatgcggcg gcagtgctcc acctgcattg
     3181 caatggaaat ggaatccgga cgttccacgg attgtcgtgc gtaaatcgcg atcatggtat
     3241 cccctcccgt acagggtatg cggcttttcg tgcaaaattg ccggtgacaa cgccgcaccg
     3301 ccgttccggg gaaagttgca cccatgaccg cgggagagat catcgcagac accatttgca
     3361 atattctcgg aggagaggag cgttgcccat gaacgacctg acaggtgagc gaatcggctt
     3421 tgtggtgacc ggttcgttct gtaccttttc cgccgcgttt gcacaggcga agctgctgcg
     3481 agaagccggt gcggaactga caccgatttt ctcggagcat gccgccgcca tagatacccg
     3541 tttcggcaca gcggcagaac gggttgccga attggaggag atctgcggca aaccggcgat
     3601 tcgcagcatt gcacaggcag agccgcttgg ccccaggaac ctgctggatc tgctggtggt
     3661 ggcaccctgt actgccaata ccgccgcaaa gctggcgaac ggcatcacgg ataccacagc
     3721 gaccatggcg gtaaagtcca tgctccggcg gcagaaatcc attgtgctgg caatcgccac
     3781 caatgatgca ctgcgggcat cggcgaaaaa tatcgggctg ctgctgaata cgaagcacta
     3841 ttactttgtg cccctgcggc aggacgcacc ggcggaaaag ccggcttcca tggtggcgga
     3901 tttcacgaag ctgccggaaa cggtggctct tgcacgggac ggcaaacaga tccagccgat
     3961 tttcgggtaa gcggaactgc tgcaaaaatt ctgcggtacg ccatattgcc gccatatttt
     4021 tttcaatgtc atgtgatata ctagaaaatg ggcgaagttc ggacgttccg gatttcgccc
     4081 atttttaggg aaacactgaa taaagcttgt gcgtaagatg cgtagtcaga tttttcggca
     4141 gattgcctag aaattccgca ggcatactgc cgtatggcaa gggatttctg ggcaatctga
     4201 cggaaaatct gcaagcagat tgcgtgcaaa gctgtcaggc gggctttatt cagtgtttcc
     4261 ttagagtaca cgcgttgttc tgggattgag gtgtatggat ttggcatgga tcattttgaa
     4321 gcaggcaatt ctcatgctgc tgctgaacgt ggtgggcatc attgcctatc ggacaaaagt
     4381 cgtggacaaa aacggcggca ggcagttttc caactttgta ttggaaattg tcacgccggt
     4441 gctggtggtg aacgcctacg ccgacgtgga gtatgagtcc cgtcttgtgg tgaatatgct
     4501 ctggacgttt ctgctggcgg cggtgtccta cgtggtgttt attgcggcag tgtatctgct
     4561 ggtgcggcag aagccggggc gggaaacgga aatcgaacgc ttttccgcca tttacagcaa
     4621 ctgcggcttc atggggattc cgctggcgag tgcgattctg gggaacgagg gcgtgtttta
     4681 taccacggca tttctgtcgg tgttcttctg cttcgcgtgg acacacggca tcatgctgct
     4741 gacagggcag cacgaccgga aagcactggt gaagaagctg tgctctccca ccatgatcgg
     4801 tatcgtgatc gggctgctgc tgttcttctt ccggattccg ctgccggatc tgctgggaaa
     4861 gactttcggc tatgtggcag ggctgaatac gcctatggca atggtggctt cgggtatcag
     4921 cgtggcacag gcagatctgt tccatgccct gaagaatccg cgggtgtatt atgtcagtgc
     4981 cgtgaagctg ctgatcgtgc cggtgattct ggcggcactg tttctgccgc tgggcttcat
     5041 tccggagtct gtccgtattg tggtgctggt gctgatggca gccccctcgg cggcaatgtg
     5101 cacgctccag tgccagaaac acgacatgaa cgatgcctac gcttcccaca tcttcgccgt
     5161 caccacgctg gtgtccatgc tgaccatgcc cgcggtggtg aagctgttta cgtgggcgtt
     5221 ggagttggtg tgaagtatag cttttactct tgtttcaagt ttcacagaag aaacccctca
     5281 gtcaagcctg cggcttgcca gctccccttt cagggaagcc taggtacaaa taagcctccc
     5341 ttgaaaaggg agggggacca gcgaagctgg tggagggatt tctccgtgaa atgcaacttc
     5401 tagtgctgcc atgtcgaaac cgtccggcgg aattcgctcg ttgccctcgc tgtccgcctg
     5461 gggcttgcca acgccggttg ttgcccttgc ctgtatcggt aatgttgata ttcgattgtt
     5521 tttcttatgc aagtggg
//
LOCUS       DAWBGR010000166         5409 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3741_length_5409_cov_3.466941, whole genome shotgun sequence.
ACCESSION   DAWBGR010000166 DAWBGR010000000
VERSION     DAWBGR010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3741_length_5409_cov_3.466941"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1922..1931
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4585..4594
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1432 a         1488 c         1141 g         1328 t
ORIGIN      
        1 agcgtcggat tgtggtcccg aagatcgtgg gttcgaatcc catctcccac cccacaaaat
       61 atcacgcaaa aaaggtataa tacagttcct gaaccggaat ttgtattatg ccttttttgt
      121 gttatcctaa caaaatccta acgtgcaaag ccaaagttag catcccccat ttcctgacga
      181 atccatcccc aaagcaaaaa aattcctccc aaccactaac ctgttgagag gaatcttttt
      241 ttaataaggt ttattacagt ttacgcagcc agaacgctgt ccataccttc aatggtaaag
      301 gtaatgctaa agctgttctt cggcatggta taatccaacg ctgccagatc agtgttgtaa
      361 atgttcacca gttccatgta gaaattgccc tgatcatcag ccattaccag cggtttgtca
      421 atcgggatct cctgatcgtc gcactttaca acaacatcgc tgactttcag cgtgttgtta
      481 aaggcaaagt ctgtgctgac aaacagcatg ttcagcttgc tgctgtcaga ggagaaatcg
      541 taaccgtcca cagaaacggt ataagtaccg tctgcggaaa tggtcgcatc tgtaaatttc
      601 gcaccagtgt caatgatctg gtcgtcctcg ccccacttga tcagtgtgtt gaagtaatcc
      661 gtgtccttgc cgtacttggc atcgctgcag gtgttccgga atgtgtaaac ttccgtctga
      721 atgccgatgt atgcatgtgc atcgtctgtc ttttccacct tattttctac gacagtatcc
      781 agaccagcct tcgggtcttt gttctttact gctttcagaa tggtggagat gatctccggc
      841 tgtgtctgct catatgtgtt gcggtcgaac agaccaaagc catacacgcc gttaaagccg
      901 ttatcccagt ataccggaat catgcccatt gaagcagcct tacctgcaac atagccgtcc
      961 cagtatgcac ggttaccctg aatctgtccg gcaaaatctg cataagccga ggacttgtcg
     1021 atacagccat actcaccaat gataaccgga atatcattct ttacgaattt ttcctgcatg
     1081 cgactgaaca gatcgtccag ataagattcg tcgccccatt ttgccttatt catcttcggg
     1141 aagccgttca cttcaatcag attatcgccg tactcgcccc acagaatggt tttcagatcc
     1201 tctataccac agtaatccca cgggtcatag tagtgtaccg aaaccatcat gcggctttct
     1261 ccagcagaac atttttcatc agtaggcatc tcaaagccat agtcccctat ggtgtactca
     1321 atgttggtgt tccagcccgg aaccaaaagc cagcggtgcg tgttcttttc gcccgtacca
     1381 cgaaccgtat ctacaaaaat ctgattgtac tgattcagat ttgcatagta ctccttgttc
     1441 gggtcgccat catatgtacc gtcagattct tcgttcatgg attcgaaaat cagatgttcg
     1501 tcatactttg caaagcgttc cgcgatctgc ttccataccg cttcatactt ttcgcgaatc
     1561 atatcctgat cttcgccgtt taccaggaac cagccgccgt caatgctatt atagccatcg
     1621 ccgtgcatat tgataatcac atacagtccc tcattatagc aatactgcac cacttcttcc
     1681 acacggtcca gccaagcgga atccacctga tagccgttcg catcatcaat gcgatccaga
     1741 taggaaaccg gcacacgaac agtcgtgaat ccagcatcag ccactgcgga aatcagttcc
     1801 tgtgtgatct ccggattgcc ccatacggtt tcgttgactt ttccgccgga attggcttcc
     1861 agctgatttc ccacattcca gccggcaccc atagcttcag cgatcttgtc cacaccgatg
     1921 gnnnnnnnnn ngacatatac ctctattata ccatagaatc tgaagctgtc aagaattttt
     1981 gtggcatttc acagcagaat tcaccccaaa atcggaatgt tgcataaaaa agcactgcac
     2041 aaactgtgac attcgttgat gcagtgcttt ctatagggat tatcgcacaa agcttgtacg
     2101 gtgctgccct catatttctt tttccagttc attatccacc aaatcatagt gagcatcgat
     2161 tgccttttcg caggcgggat aatctccgcg gcacaacgcc tgaaacagca tggtatgtga
     2221 ttcctgtagc ttttgcttga cagtatcact tgccgtctgc aatgcggtat cgatccactt
     2281 gcggtaaact tctgtaattg gttccagcaa tgcgatccag aactgatttt ccgtggcatg
     2341 gatcagcaaa aaatgaaatt gccgatccag ttcgatctgc cgatccagcg gctgttgttc
     2401 ctgtgccttt tgcagcaagg cggctgcctg ttccttccag cggaaaaagg tgtcctgatc
     2461 cagaatcgcc cgacagactg ctttttccat attccgccga aacgagcaga tctcttttcg
     2521 gtcagtctgt cccagcaaca gcatcatatg taccatttcc gtgattgggc gggatacgtt
     2581 tccggtcaga tagttgccgg agccacgctt gcagacgatc acgccggtat gctccaacat
     2641 tcgcagagcc tctcgggtag agtttcgccc gatcgacagc gtttccgcaa gactgcgttc
     2701 tgtaggcagc cgagaaccaa tagccagttc tccgctggcg atcatctggg acaaataaga
     2761 gatcactttc tgatactctc tggtttcttc ctgcataagc cacctcacat gatatgtgaa
     2821 aaaatcgttg ccccgattac ggtaatcaaa ccggcaacta cgatggacag gctgctcatc
     2881 gcaccttcca cttctcccag ttccattgcc tttgcagtgc cgatggcgtg ggaagccgtg
     2941 cccaatgcaa tgccttttgc cacaggctcc tgaatccgga acagccggaa aacgatttcc
     3001 gcaaaaatat tccccagcac accggtaatg ataattgcgg caacgctgat agacacatag
     3061 ccgcccagct tttccgaaac gcccatgcca atggcagtgg taatggactt cggcaaaaac
     3121 gttacatatt cctcatggct gaacctgaac agcactgcca ttcccagaat tgcccccagg
     3181 ctggacaaca cgccggacaa gatgccgatc aggactgcgt gccagttctt cttcaacaat
     3241 tccagctgct cgtacagcgg aatagcaagg caaacggttg caggggtcag cagatagctc
     3301 agatacttcg caccctcatc gtatttctca tagctgattc cggtacacgc cagaaatgca
     3361 atggtgataa taatggagat cagcagcgga ttcagaattg ccagcttgaa ttttttcttc
     3421 agcagcagtc ccaggccata agatgccacg ctgatcacca cgccaaaaaa cgctgaattt
     3481 tccaaaaagt caaacatgat tttcctgcac tccttttttt ctatgtcgaa tgactgattg
     3541 tgttacctta ccggaaataa tcatcacgat ccatgtggaa agcactgtga ccgccacata
     3601 ctccagccac gccggtgcga tcacgtccca tgtttccagc aagcccaccg ccgccggaat
     3661 aaacatcagc ggcatgatct cgatcagaaa ttttcccgtt tcatgcacgg attcatgggg
     3721 aatgatccgc agcttcaggc agagaaacag cagtatgatg ccgtaaatac tcgccggaat
     3781 cggcagcggg atccactttt ccaacacctc tcccacaaaa gaaaccaaca gaatgattcc
     3841 aaattgtttc atccatttca tttgacaaac acctctttaa ttggtactac caatctatta
     3901 taacacggag attatgacac gtcaagcatt ttacagtacg aattttatgc agaaatagaa
     3961 aaaactgcca tatgcctttg gagcatacag cagcttttca aacgcactca ggcagcagcc
     4021 gatttccggc acgttcggac gcgtacccag ataaaaaaca cgatatagac aagactggtc
     4081 agtatccacg tgaccggata ggacagataa accgtttcga ttcggtgata ttccggaatc
     4141 tgaaacaccg tcgccagcca caccagacgc agcacacaca cgcccacaat ggaaacgatc
     4201 atcggcataa cagaatatcc gataccacgc agtgctccca ccataacgtc cataatgccg
     4261 cagagagcgt aaaccataga gatatagtgc agacgcacga ttcccgcggc aatgacttcc
     4321 ggattgctgg agtagatgtg aagcaattcg tttccgaaaa atacaaccgc atttcccagc
     4381 agcgtaccag tgacgatcac gcaggcttct cccagaagta cgattcgatg gattcgcttc
     4441 tgttcccctg ccccgtagtt ctggctgaca aaggaaaggg tggtctgata aaaggcgttc
     4501 attgccatgt acacgaaccc ctccagattg gcagcggcag agtttccggc aaccacgatg
     4561 tttccgaaaa tgttgaccga ggacnnnnnn nnnnccacga ccgtggagcc aaaggaattg
     4621 atagccgact ggatcaccac gttggacaaa gcgaatacca cgccctgaaa tcccgccggc
     4681 aaaccaattc gcaaaatctg tatcatcttc tttcgatgaa tgtgcagagc acgcagttcc
     4741 agatgaatcc cgccagactc tttcatcaga caacgcagca ccagcaatgc ggaaacggtt
     4801 tcggagatca ccgtagcaat ggcaacaccc attacgtcca gatgaaagcc gactacaaaa
     4861 atcagattca gcaccacatt gataacaccg gcacagagca ggaaatacag cggccgtttg
     4921 gtgtccccca ctgcccgcag cagagcactg ccaaagttat agaccattgt agaagccata
     4981 cccagaaagt agattcgcag gtatgtggtt gccagcggaa gcacctcctc cggtgtctgc
     5041 atccagatca gcatttgtcg ggcaccgatc acgcccagca ccatcagcaa gccgccgctg
     5101 atcatgctta acagcataga tgtatgcacc gtgtcccgca gatcgtccca cgtctttgcc
     5161 ccgtaggaac gtgccgccac aacatttgca ccaatggaca gcccgataaa caagttggtt
     5221 agcagattga tcagggcagt gtttgagccg actgccgcca gcgaattgtc accggcaaac
     5281 tttcccacca ccacaatgtc cgctgcatta aacaacagct gcaaaatact ggacagcatc
     5341 agcggcagtg caaacatcag cattttccgc aggatcggac cgctgcacat atccatttcg
     5401 tattgtttt
//
LOCUS       DAWBGR010000167         5392 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3752_length_5392_cov_4.992693, whole genome shotgun sequence.
ACCESSION   DAWBGR010000167 DAWBGR010000000
VERSION     DAWBGR010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3752_length_5392_cov_4.992693"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1276 a         1523 c         1408 g         1185 t
ORIGIN      
        1 aaaatttgac gacgcatctg tcgcacaatc tttgtgcggt attgccataa tgaaacgata
       61 aaaacggaca gtggcaaagc gacaatcgcg gacactgtcc gtttttttga aaggagcatt
      121 tttgatgcag gagccgttcc cgaattttcc cactgagccg ccgcagaagc ctgccggcga
      181 accgcttccc ccacaggaag aaacaccggc accgcctaca gaacagccgg ttccaccagt
      241 gcaaaatcca ttaccgcctg tacagaacaa ccccgttccg ccagtgcaaa atccattacc
      301 gcctgtacag ggcatgccgc agaataatcc gattccgcca atgcagaatc cattaccgcc
      361 ggtacagggc accccgcaga acaaccccgt tccaccagtg caaaatccat taccgccggc
      421 acaggacatg ccgcagaata atccgatccc gcctatgcaa aacccaatcc ccaactggta
      481 ccagaaccag cagcccacca tgggaaactg gcagattccg ccgcaggtac cgcctgcacc
      541 gccgcaaaaa cagcctctga caccggaaca gaaagaccgc cgcacagcgt tctggctgaa
      601 aatcgtagcg gctctgctgg cagccctgct gctgtactgt atcggcagcg atgtggtttc
      661 ctaccgccgc ggcggtgctg caccggctgc tgccacagct tcggacactg acagggacga
      721 aaccgccggc agcaaggtga tcattcagca gcaggataaa cccgaattgg acaagaacgc
      781 agaaaacacc gatgcagatg gtgcctacac cgtagagggc gttgcttctg tggtgtgtcc
      841 ctccattgtg gaaatcgaaa cctacggcga tgccaaaggc gaacagctta gcggcaccgg
      901 ctccggcatc atctccagcg aggacggcta catcatcacc aacgcccacg tcctgactaa
      961 cgggaagtct tttcgtgtca tcatgtccga cgaaacggag taccctgcca ctgtcattgg
     1021 caaggacaac aaaaccgacc ttgccgttat caagatcgag ggcagcggct tccctgccgc
     1081 cgtcatgggc aattccgacg aggtcgttct gggcgaatcc gttgtggcga tcggcaatcc
     1141 cgcagggctt tccggctctg tcaccaatgg cattgtgtcc ggtctgaacc gacagatccg
     1201 ctctgatgcc accggattcg acatgaactg tatccagacc aacgccgcga tcagtccggg
     1261 caactccggc ggtgcactgg tcaacatgta cgggcaggtc atcggcatca cctcctccaa
     1321 atacgtcagc tccagctatg aggggctggg atttgccatt accattaacg aggtgctgcc
     1381 cattgtgcag gatctgatcg acaacggcta tgttgccgac cgtgtgcggg taggcattac
     1441 ctttatttcc ctgatccccg aagaagtccg gcagggcttt gccgacgaga tcggcatgaa
     1501 aaccgtaccg gataacctgt ccggcgtgtg gatcacggaa attgcagatg actgtgacat
     1561 tgccagcact gacctgcaag tgaatgatgt tatcgtatcg gtacagggcg aagccgtaga
     1621 cgactacgac tccctgaatg ccgccattgc cggactgaaa ggcggcgaca ccatagaggc
     1681 acgctgccgc cggtaccagc cggacggaaa ctttgaggag ttctccattc agtgcaagct
     1741 aatggcagac acctccggaa actattaagg caacaccgca caaagcgtgt gcggtattgc
     1801 cttaaggaaa cgcaatttta tgtaaaaaag cgttcagagc agatgaatga acctgctctg
     1861 aacgcttttt gcatctgatt cagcctttgg cgaaattctt tgccaacact ttcactgtcg
     1921 gcagacacgg cacatgtgct ctgccattct cactgtccgc gtttctgtgc cttgttcagg
     1981 aaacgttccg catcgaccag aaaccgcttg tgcacgccgg aaccgatcac gcctacgccg
     2041 tcctctgcgg taacgtgata ggagatctcc cgcccgttga caccggtaat ttctgcggta
     2101 gcagttaccg tcagccccac cggcgttgcc gcatcgtggc tgatgtccag cttggtgcct
     2161 acggtggttt cgccctcgct gaggaacgga gccactgcat tgcaggcagc ctcctccatg
     2221 agggctgcca tcatcggggt ggcaaatacc ggcagcgagc cgcttcctac agcaactgcc
     2281 agattggttt catctacctg taccgaggcg gttcccttgc ttccaattgt aatcggttcc
     2341 atacatattc tcctttagtc cgaagtcgtt tccgtggttt cttctgtagg atccggcagc
     2401 acatctaagg cactggggga aacgatgtcc ccaaggatcc ggtcataagt cagcgtatcc
     2461 agctttttgt ctgcctcctg catgggcatg tagttggtgg agtacaccgg ctcagtttcc
     2521 atttcgttca tcatagtctg ggtgaagctg tcacggggtg catcgatcag ctgcatcacc
     2581 agatacgggg catagtatgt ggaaaccgct ttcgacggca gcaaactggt gttcagtccg
     2641 tagttgttcc acaggatata tcgctgttcg tacagggtgc tgcaattttc gctggtgatg
     2701 ttcagctggc tgtagatgcc gtcgtccccg cggagggacg ggaaatggtc acccacaaac
     2761 agtacaatag tcggctcgtc gatctgggag agccgttcca gaaagtctgc cagaccgtct
     2821 gccgaatgct ggatccggct caggtagttg ataaattcat catcgctgtc cccgtcagaa
     2881 tagggctgat ggttctgcat ggtagtggca tgcacataca gcggctcgtc agtagatgcg
     2941 atcagagatt cgatctggtt gtacaccaca ttgtcgtcga tatattcccc gtaggtcgtt
     3001 accggaacgg tgaagtcatc ttcaaaaatc atctggtcaa agttgaactg tccatagata
     3061 cgtttccggc tgtagaagct gctctggaat gggtgcacat aggcagtgga atagccccag
     3121 cttttataat atgcaggcac tgtgggctgc tgccgttcca gcatcattct ctgcggcatg
     3181 ttggggtcat tcagggactt caccggcagc ccgaacagca gttcaaactc tgtccgtacc
     3241 gtatagctgg cgtaagtcgg cacgatcagt gtcccctctg ttcccataga agcggctttg
     3301 tccagtcccg cataataggg atccagattc gtatagccca gttccgacag cttatcggaa
     3361 aaggcacgga aatccgcaaa cgattccgac atgatctcca ccacgttggg cttcacgccg
     3421 tcatcgaagt ccagatccgg cacttctgtt gccagatact gcttcacggt gtcgctgctg
     3481 tcctctttat aatcctccgg ctcctccagc tggttggaga ggttctccga acccgtctgc
     3541 ataaagaacg ccagaaaacc gttgttgtcg aacttttcgt tcaggataaa ggtattgtct
     3601 gcctctttgg tgtccagacg gaacagggcg taaaccggct gggaaattgc cggcacggta
     3661 acgaccatga cgcaggcgat caggcaagcc agaccggggg caagccgttt ccgcagcttc
     3721 agccgcattt tcagtcttgg gttgaaccag aatgccagca ggataaacgc ggcacaaatg
     3781 gcaatgtaca agaccagcct tggcgtaatt ttgatatagg cgaatgaagt cagacttttc
     3841 agactgcgga acagcttcat gtctgtcatg atcaggtggt tgccgttggt gttgtacttg
     3901 aatagttccg taatactgag agccatatac aggatacttt ccagcagcat agagaaccag
     3961 cccgacttga acaggagcag tgccccgata aaaatcagtg ccgcgatcag cacgttaaac
     4021 agcatgatcg tggggtggtt ggtgatgaac agcaccaact tactgggata tttgtcctga
     4081 ttcagttccg ccatacacac gatgaaaatg ggatacagca tcatcagcag ccggttggtc
     4141 atgggataac gcagcgtatt gtaagaacgt ttctcatgca cggaacagaa ccatgcacgc
     4201 agggaagaaa agcgtgcata ccatgtcaat tgttctttca tgtttgtgag atcacctttg
     4261 tttcaaaaaa gaattgcaga agtcagaatt cccctgattt ctgcgggatt gtcgtaacaa
     4321 tactatagca caaacccgcg gttttcgcaa gtattctcgc caaaaactca tgcagttttc
     4381 acggaatttg tggattttat cgaaaaagtc atgccccgaa aaaaggtttt gtgcaagttg
     4441 cagagggcaa atttcccgta tttgggtgaa aatcggcact tctcctgccg tctgtcagcc
     4501 gcaccagaaa ccgccgttta caggaatatc ctgtccggtg atatacgccg cctgttcgga
     4561 caccagaaat cgcacgacat ctgccacatc ttccggtttc cccagccgtc ccagcggtgt
     4621 ttcctctgcc agttcgtcca gtgtttcctg cggcaggtgg gacaccatat ccgtagcgat
     4681 cgtccccggc gaaacggcat taacgcgaat gccggaggga ccgacttcct tcgccagtgc
     4741 acgggtaaac gccagaatcg cccccttggt cacggagtaa tccacttcac aggaagctcc
     4801 ggtttcgccc cacatggagg agatattgac gatacagccg gcatggcggg agagcatcga
     4861 cggcagcagc aggcggctaa gttccaccgg agccgtcagg ttcaccgcat agagccgccg
     4921 cacctgttcc ggcggcaggc actggaacag attgatatac gagcatgcgg cattattgac
     4981 caacacgtcc acatgtccca gatgttccag cacggttccc gccagttcgg gcagggcatc
     5041 ggtctgtgac aggtcggcgg caatggggat cacgccgcag gcggcagcca gttcttccat
     5101 ggcatcgcgg ctatggcagt acacgccagc caccagatac ccgtctgctg ccagaccctt
     5161 tgccacagcc gccccgatcc cgcgggacgc accagtgacc aatgctgttt tcagcatatg
     5221 cagcacctcc gttccaaaag tctattttcc agaaaaatta tagcatgcac aagaaaaaaa
     5281 gtcaagcggc gggcggcggg cggcgggtcg ccgctgaccc tacgccccaa tttcaactgt
     5341 aaaaagggta atgttacggc ggcagcagcc gcctgtatgc cccgccgggg ga
//
LOCUS       DAWBGR010000168         5387 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3754_length_5387_cov_3.474681, whole genome shotgun sequence.
ACCESSION   DAWBGR010000168 DAWBGR010000000
VERSION     DAWBGR010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3754_length_5387_cov_3.474681"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1132 a         1427 c         1439 g         1389 t
ORIGIN      
        1 tttgcaagca gatcgcgtgc aaagccgtca ggcgggattt attcagtggt tccttaaaaa
       61 cggcagccag ctgtccagca gccgcatcgt accttgttcc agttcctccg gcgaaagaca
      121 agcgtagccc agtacgatct gcggcgtggg cggcactgcc tgcggcgttt catagtagtc
      181 ggaaagccca tagacaacaa cgtgtgctgc tgcggcagct tgtaccagtt cctgttccgt
      241 gcagggcagc tgcacttcca gcaacaagtg cagtccggca gcttctccgc agatgtgcag
      301 atacgctccc agcgggtgag agcgaagcag ttctgtcagc cgcttttgcc gttggcggta
      361 ggtctttttc atgcggttaa ggtggcgttc aaaatagcct tcctgcaaaa aatgtaccag
      421 cgtgtactgt tcaaaacagg ggacactgga cgaatagaaa gagaagtcct gttcgaactg
      481 ttttgccaaa ggcggcggca gcacaacata accgattcgc agcgatggtg caagggtacg
      541 ggcgaacgtg ttcatgtaga taacgtgccc actagtgtcc atgctgtaca gcgacggcag
      601 cggcttcccc agaaaccgca gttcgctgtc atagtcgtcc tcgatgaggt agcggtctgt
      661 ctgagcactt gcccatgcca gcaactctgc ccgtctgcgt accggcatga ttataccggt
      721 ggggaactgg tgggacggtg tcacatgtgc cacggaagca ccggattgct gtagggctgc
      781 cagttgcagc ccattttgat ccatcggcac atcgatcacc gaggcaccgt tgctgcgtag
      841 aatgcgttta cccttggaat agccgggttc ttccacggca tagcggcggg acgcacccaa
      901 cagctgcacc agaatcccca gcagatattc cgtaccggca cccaccagaa tttgctccgg
      961 tgccgcatga atatcccgaa acgcccgcag gtgagccgca attgccgccc gcagttcgta
     1021 caagcccatt gccggtggcg attgcagcag cttttcctgt ttggaggaaa gcacctgtct
     1081 ggaaagcttt gcccatgtgg caaagggaaa ctgctccaga tcgacaccgc tggtggaaaa
     1141 aacaattcgg tcatccgggt cagtctttgc cggctgtgat gagggaaaag ccgtttcttt
     1201 tggcatatgt gccagcagcg gtgcatccgg atccacaaag taaccggcac gtggacggga
     1261 atacagatac ccctctgccg ccagctgttc ataggcagtc tgtaccgtca ctatgctggt
     1321 ttgcaagtgt gtggaaagag cacggcggga agggagtttc tcgtcagggt gcagaacgcc
     1381 ctgtgcaata tcctcccgaa tggctgtgca gatctgctgg tacagtggtt caggattgct
     1441 gggattcagc agaatggtca gcatcttggt cgattccttc gccgttcagg aactgctgca
     1501 tcttgtcggc agaccattcc atttctacat cagaagcctt ggcagctttc gcctgttcct
     1561 ccgaagcgtc aaagatctcc gaaatttctt ctatagactg attggtgagt gcatcatatt
     1621 ccgacgaatg aggcgatgtg ccggatagta cgtggaagat gtcctccgcc gacagttcac
     1681 ttgcagcatc ttctgtagcc ggctgttctg tcggattttg ttccggagca gccggttctt
     1741 ctgcctgcgg ttctgcaaca gtatcttctg tttccgttgc tgccgcagat gcttccggag
     1801 cagcctctgt gaaatctgca tcatctgctg ccggtacatc tgtattttcc ggaatttcag
     1861 tttccggagt ttctgcggtt tcttccggct gttccggtgt ttctgtcggt tccggctgct
     1921 tcggcggctc tgtgaatggg tgtttcaggt gccagagcat ttcgtctgcg tccatgaagt
     1981 ccgcactgtc tttctgtaca gcctgtgccg cacgtttttg ccgcagttca gccgcaaccg
     2041 tagaacgaat atttgcagga acggtaagcg tatctgtcat gcggggatac atctccggaa
     2101 tttccagtac gtccagcttg aacttgccgc cggtgataaa gacacgcatg ctgtgtgtgc
     2161 cttccggcag ctggtcaatg accagacaag cctgacgggg cagacagctg ggaacaaagc
     2221 aattttcgta catggtgtgt ccgtcgatca ataccgtaaa ttctgcattc tccgcagtac
     2281 caacaatagc aatgccgccg ccctcaaagg tacaggagaa ttccgcaccg gttttcgctt
     2341 ccatagaggt gcggttgtag aagtggtagc tttccatggc atgtacttcc cagttttcgt
     2401 tgtacaagat gccgtttccg aagcagtcca cacgtcggac atacggcggc tgtaccgaag
     2461 ccggctccag gtgaatgttc cggaacatat tccatgcgta ttcactgcaa aggctggcac
     2521 gaccgctgtt gatcatgcac tgcggcgtat actttgccag gaaattgccg ttgatgaaga
     2581 agaagatcgc gttgcccagt accaccagtt tcagcttgtt ccactgtttg gggtcaatgt
     2641 gctgcgtaat gccctctgcc gcaacacttt ccatatccat taatttccag ctgccgtcgc
     2701 cgtaaagcct gccgctgtag ccgcacatgg aagtgaactc acaggtgaca gtagaattat
     2761 agcggatacc gattccggcg aagttgtgtt cctctagatt ggaaagcttc acttctgcct
     2821 ccatgctgta gcttctccac ttgtcatcgc cgaagcaggt cagcggcagc ggtgtcccgc
     2881 ggaaccgcca gttggtgggt atgatctgcg gcgtgatctt ctggcagatc gcaaagccgt
     2941 cctctgtttc taccaactcg aacgcaccgc cctgatcggt catgtatctg ggagcacagc
     3001 cgcggacagc aatttcttct ttggtgtact gtaaattttc cgtgtaggga agtgccaggc
     3061 gggaggacag atgcggctgt actgccggaa atgtgttcac gccgtccacc cagctcacgt
     3121 ccatagtcgt cactgtcata atggaacgtg gcggcacctt aatggtaaat gtgtcccgat
     3181 gattccggat cggccggatt ttcttgccga tgcggaacca gttggcatca tacgcctgtc
     3241 tgccggaagg tcccttggtg ataacactgg aaaatacggt aggctcaaat gccatatcct
     3301 tgacttgcac cgtataaatg cgaacgtcct cgctttcgtt ggtgaagaac atggacattt
     3361 cgctgcggtc cggtgccgtc agcgtcatga aattgtgagt ggtgttggca atggcatgat
     3421 tttcctcgcc gtccccgtag caggcaccgt tgacatacat ccagcccttt ctggcaaagc
     3481 ggctgaaatg cattgccagc cagaaaccaa tatccagaac aaagtgtccg gaccacggct
     3541 cccaggcccg aatcagctgc ttcggggcat agcaggcacc gtcatagtaa gcggcaactg
     3601 ccggctggaa ctcgtacatg accattttgc cgttgtaata gctgttgatg atacggtttg
     3661 ccacatcaat aggaccgttt ttgcctacca gtccggactc gtctgcctgt actgtcagtt
     3721 ccggtacatt gcagggggca ataccctcgc tgtaccagat ctccttgccg tatgcttcgt
     3781 tcagcagatt ggtataagaa tccccgaatg tggtgtagtg caagccgatg acatcgaccg
     3841 catttcgcag aacgctgttg tccaccattt gttccgcgat gttccgcgtg ccgacctcgt
     3901 cagaggcgat cagcttgatt ttgctgtagt catagggggc atttttttcg ttgtccagca
     3961 tgtgccgcag gtagaggatc cacgcctcgt ccggtgtatc cgtttcgttc tggtctgcac
     4021 tgatatagtc gaatttcagc ccgtacaccg cataagctgc gtccagtgtt tccttgtacc
     4081 agcggtatct ggcacgcagt ccgtgttcct gactgatgac aaacgcatct gtgacccact
     4141 tcggctcgcc ccagcggagc atgtcgatgg taatatccgg attgattgcc ttggcatctg
     4201 cggcaaactg gaatccggca ccgcgtgtca cgtctgccgg ttcattcagg gaacgcttgg
     4261 tgcagggctc tgtgccggag gaggaattga catctgctcc cagttccagc ttgatgtgtg
     4321 tcagacctgc accataatcc ttctgaaaca gcagtcgcat gatctcctca taggcatgag
     4381 ggtgttctac tttatagtcc agcagcaagc gggaggaatt gttgccgctg acacagccca
     4441 gtccacgata acaagcggca gggttttcat ttgcctgtgt gccgtctgca aggacttcca
     4501 tcggtgtaat ggttgagcgt tctttgaagt ttgaaaaatg gttgacaatc ttgttgtttt
     4561 tcatggaaaa gtccattgtc atcgtttcct ttcgctatat ttttcactgt attgcagcag
     4621 ttcccgcaga gattcttccg gaacactggg atccggttct gcaatgagaa acttgatctt
     4681 ttttcccatt tcgtcgaaca tgtgttcgtg ttcagtttcg aagtaccgca aagtcggttc
     4741 gctatggaga tcacgggtca ttgtcgtaat gcggaatccc gcttccggaa aatattccag
     4801 cgactcctca aataattcgt catcgtcagt tttgaaccag atttccccgt ccagcagcat
     4861 gcggtactgc accagctgcc gcgggtgtgt cagccggcgt ttcttgtgct tgccccgcgg
     4921 ccacggattg cagaaattga tgtagattcg atccagccgg tctgtttcag caaaacattc
     4981 ccgcaggtac ataatattat cgatcataat gcggacgttg tccagcggca gctgctgttc
     5041 ggcataggcg gcttccagct ttcgctttgc cagtaccagc atttcatttt tgatgtcaat
     5101 ggcgatgtaa ttgtattccg gattggcagg agcctcctgt gcaataaagc cgcccttgcc
     5161 gcagcccagt tccagccgca gcggctgtgt ctttggaaat tgtgcctgcc attgtccttt
     5221 gcaggctttg ggatcagaga tataaaacgg gcatgcttcc agttccggct ttgcccaagg
     5281 tttacagcga attcgcatga aaaagtgtca atcctttcgg tttctaggga agcactgaat
     5341 aaagcttgtg cgtaagatgc gtagtcagat ttttcggcag attgcac
//
LOCUS       DAWBGR010000169         5359 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3775_length_5359_cov_5.243213, whole genome shotgun sequence.
ACCESSION   DAWBGR010000169 DAWBGR010000000
VERSION     DAWBGR010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3775_length_5359_cov_5.243213"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1541 a         1202 c         1238 g         1378 t
ORIGIN      
        1 gaaaaatctg actacgcatc ttacgcacaa gctttattca gtgtttcctt aaaaatgtca
       61 ggaggaaaaa tatgaacatc tttgaaaagc cgatcaacaa agaatacgtt agagaatatc
      121 tttatgattt gaaagatgat tttgatggag aggaatggaa tgaagatttt gaggaattac
      181 cagatgtatt ctatcaaaat gataccgtaa ggatttcaca tctatccgaa aagaacgttg
      241 tggaatgtgt ttttttacaa gatgatgtca atgtttattt tgctttcata aatatcgatg
      301 tttctgaaaa cggtttacaa aacgccttga cagccatttc ccggtcagat gctgattgtg
      361 ctgaaaaatt cgatcgcatc tgtgaattga tcttccacaa aaaatttgac aggtcgctcg
      421 aaaagatcga tgaatatgga ctttccctga gatgttctgt ctatagcgaa attgctgaaa
      481 aggataatgc aaacgctcaa tatgaacgtt taggcaacga gtttgcagtt gatgttgggt
      541 ttcattttaa caatgcaaag gttaatctat gctttttctc agaagaaatt gctgccattc
      601 gggaaaatca taaaattgca gagttagcaa accggataac ttcttatgat aaaaaactga
      661 gggattgcct taacaatctc tatcattacc tataatggtg tcgagtagcg taaacttata
      721 aaattcttat attttgaggt gaaaatatgc caattatgat tgaaaacatc aacgcccaga
      781 cgatcacccc ggcactggga aagaatccac gacagctcta tgaacatcag gaagaggcaa
      841 tccggaagtt agatgccatg gacaaaaaag ggtcattccg taccctgctt gttttgccca
      901 caggcggcgg aaaaacgtta acagccgcat attggctgct tcggaatgca gtagaccaga
      961 acaaaaagat tctctggctt gcacacaggc atctgctcct ggagcaggcg gcagaggctt
     1021 tcgcccgaaa cgcctatact gatacaatgg taaaccgcac ggttttcaat taccgcatca
     1081 tttccggaat gcacgataag cccgtacata ttcagaaaac cgaccgtatt cttattgcca
     1141 gcaaggacag catgatccgc agtctggata aactgaaaaa ctggctgaat ggggaagaaa
     1201 tctatctggt gatcgacgag gcacatcacg ccgtggcgaa atcctacaag aaaattatcc
     1261 agtatgttgc agatcacgcg aaatccatga agctgctggg actcacggcg acaccgttcc
     1321 gtacctcgga ggacgagcag ggtgctttaa aacaagtctt tacagatgat attgtgtaca
     1381 aaactgacct cgatgccctt ataaaaaagg gtatccttgc aactccgaaa ttcaaaaaat
     1441 ggaatacaaa tctacagttt acggagcact taggcgttaa ggcgttgaaa agcatagaaa
     1501 atctggatat gctgccagaa aatatcgtta atgatattgc aggaagtaag gaacgtaaca
     1561 gaattatcgt tgatgaatat ataaataact atgaaaaata cgggcctacc attgtttttg
     1621 cactgaataa aaatcacgcg attaccttaa atgcactttt taacgaaaag ggaaaaaaat
     1681 acggtatcaa atcagagttt atcatttccg aagtacagga tatgataaca ggcatcacca
     1741 tttccaatgc agacaacgaa cgcaaaatcg aagcataccg caatggagaa atccaggtac
     1801 tcatcaacgt caatatcctc acagagggaa cagacctgcc aaagacccat acggtatttc
     1861 tgacacgccc caccgtttcc acaacactga tgacgcagat ggttggaagg gcacttcggg
     1921 gtcttaaagc tggcggtaca aaggaagcat acattgtcac ctttattgat aactggaacg
     1981 ataaaatcgc atgggtaaat ccggaaacac ttacggaggc agagtatcac gaaaaagaga
     2041 cccttgccga gacccaaaag cagcagatcc ggctgatcgc aatttcaaag atcgaagagt
     2101 tcgcacgaat ggcagatgct gccgtggata cgtccgcatt ggacagcacg ccggcaatcg
     2161 aactgattcc tcttggtatg tatatgctgt ccacgctgga atgcaatcac cagattctgg
     2221 tatacaacag cacacagaat gcctaccaga gcctgatccg tgacctgccc aatctgatgg
     2281 agcattacag cattgagggt gaaaccattc cggaagaaac gctggacgac atgaccgagc
     2341 attgtttcca aagctacttt gacgaaaata tgattccctc ctgcaatcgg aacgacatcg
     2401 agcatctgct gaagttttat gcacagaagg ctgttgcacc tctgtttgtg acgatcgatg
     2461 aaatggagcg aaaaaagctg gatgtttctg aaatcgcaaa aaggatctac gacgaagata
     2521 tgcgtagaag cgagaaaaat gcctatattc agagcctttg ggcggaagag ggcagcctgc
     2581 tccctgtgta ctataccaac ccgtacttct ttaagaagct gatcgatctg gaactggaca
     2641 agctggacgg agacattgaa attgcagcag cagaaccgca gacagaagca gaactgcgaa
     2701 atctcgaaca gttaccccta cagaagatca ttgaactgta tccgaaaatt ggcttgcaac
     2761 taaaggaaga cgcttttgct gctgcaagaa atgatgacgg cagctatgta tgtgccggct
     2821 gcggtgaagt atttccgaca cggctctttt tgcaggtaga tcatattgtc cccatggcaa
     2881 aaggcggtct gagtgttccg gataatttac aggtactttg cagaacctgc aaccagcgta
     2941 agggagatca ctgatgtcac atacagcata ttggatcacg ccagacgatg ttgcggtata
     3001 tctatttctc gaaaatacgt ctcaccaaag agaaaacgag attttatggg acttgtttga
     3061 aaatcacaag gcacatatcc cgacagaata tggcagcaca tatttgcagt tcaagcagag
     3121 cgtcatgaat ctgctgaaca tttacgaact ggatgcagtt tcctatgacg aagcggcact
     3181 gatcctaatg gaaatagagc atacttcgct ttattccgag gaagaatccg attgctttga
     3241 cgcttatttt aagctgatct ggctgcaatt gaggtattcc ggcatcgcat accggaaagt
     3301 gaaactgaga aagctgctgc gggattttgg ctacaaaaga aggtcggaaa aactgacaag
     3361 ccgcatacag caggcaatcg acaagctaga gctgaaaacg tacctgcggg gatatgtgcc
     3421 atgcagcatt gatgctattt cgcttgagga tgtgattgtg attcggttgg gtgagggcag
     3481 tatgttgtaa cagagcggta ccccatatcc taaaggcaac accgcacaaa gcttgtgcgt
     3541 aaggtgcgtt gtcagatttt tcggcagatt gcctagaaat tccgcaggca tactgccgta
     3601 tggcaaggga tttctgggca atatgacgga aaatctgcac gcagattgcg tgcaaagccg
     3661 tcaggcgggc tttgtgcggt gttgcctaaa cactgcggct gcctgcaact gcacttggaa
     3721 agactttgaa aatcgaattt gtatgagaca aagagcggac aattaatggt tgtccactct
     3781 ttttttccga tatattgaaa ctcagccatt cacctgctgc ggccataccc attgtcaagc
     3841 aactttggct catattttta ttcgcaatcg caatataggg tgcatttgag tatagtataa
     3901 taaggatttg caccctatag agggggcttt catatgtgtg atttttcttg gcagatgttc
     3961 ctatatgtct cctcttccaa tagagcttga tggtaagaat acaagtccag aaagagcctg
     4021 acttcataac aaaaaaagaa aagcctgccc ccaacagggc agacctttcc ttctatccca
     4081 aagcctcgca aatcctcagt gattctgaat aatctcgtta aaatagccgt cgatcacgct
     4141 gtccgccttg ttgcgctcgc tgtcgaaggt ttcctggtac acgttcttat acacatccgg
     4201 tgtactccag ccgcccatct ccatggcata tttctctgga atattcagca ttgccgtggt
     4261 gctggcgaag atatgccgca ggtcgtgaaa acggatctct tccagaccgt gtgcctccag
     4321 caatttcttg aaccgcttgc ggatggtgtt tggtgtgaac ggaacaatat gatcggttcc
     4381 cgactgatgg ggcacttgct gtatcagtgc ataaatgtat gccggcagat tgacatagcg
     4441 catactcttg acagttttgc agtggtcgat ctctgcccag ccgctgtcca ccttgacgat
     4501 ctcccggcgt acatagagcc gcatctgcac cgggtcaatg tcacagaact gcagacctgc
     4561 cacttccccg atgcgcagtg acagccacat ggcaagcaat accggcagtt cgctgctcgt
     4621 ccccttgacg atggcataga tcacatctgc ggacgggagt cttcgttccc gcacctcctt
     4681 tttcggcagg cgaatgctgc ccattttgat agtaatatca ttggcattca gcaccgcgcg
     4741 caggaatccg actgcgttct tgatggactt ataaccgaga cgctgagcgt ccacattgat
     4801 ggcgatttga atgtcacggg cagtcagatc ttcgattttg atgtccatga tgtactgcag
     4861 cttgttgcgg agcatttccc gataacagca cagggtggtg ggttccagca ctctgcttct
     4921 gctgtcaata aactgcagca tagcttctct cacagtgtaa ggatttcgtt cctcttcttc
     4981 gatgcccttg agccactttg tcgctttgaa atacacttcg tcctctgtgc tgccggtaaa
     5041 cattctgcgg tttcgcttgc cgtccggacg aacgcccttg gacacctggg tgaaatatgt
     5101 gccgttttct gcctgctgaa tgaccagttt tgtctttttc attttcataa gctccttttc
     5161 aaaatgtgca ggctctaata taccgcctgc tctgtgatta cgccgccaat agatcgtcca
     5221 gtgtcagatc gttgatgcag aggaacgtgt ccaccatttt cagcgtaagc cctctcgcat
     5281 catgatcgta gttctggagc gttctcgtgg aacagttcag atacatcgcc agctcgtcat
     5341 ctgtccagtc gtggagcag
//
LOCUS       DAWBGR010000170         5347 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3781_length_5347_cov_4.277211, whole genome shotgun sequence.
ACCESSION   DAWBGR010000170 DAWBGR010000000
VERSION     DAWBGR010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3781_length_5347_cov_4.277211"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1213..1222
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1221 a         1498 c         1284 g         1334 t
ORIGIN      
        1 cctatgaccc tctgcttgta aggcagatgc tctcccagct gagctaagct tccatgattc
       61 agcgacgaaa tttattatac catatctttc ggcatcatgc aagctgattt cgcagggttt
      121 tcaaaaaatt cacatttcga aactgacaaa taaaaactgc ctgcaccgag ccggcacagg
      181 cagtctatca ttccatgaaa tcgcgaagcc gcaattacat accagttacg ttgaaagtta
      241 cttcaatggt tgtccagtcg ccaatagcag acggatccag aatggttgca gaatagtcgg
      301 acttgtttgc cagatcagca atgttgccct ctgcacttct tgcatcgccc ggcatgccgt
      361 cgtccggtac ccactcgttg aaaatgttgg aacgaatatt gttgtgcttt acgccgttga
      421 tctcggtttc ttctgtattg gtgaagcttt tcttggtcag cgggatctca gtaccgtcta
      481 ccttgacagc agttacatcg atcactgcgt tcggggtagc agttgcaccg tccatgatct
      541 gtacagccat gaatgcagtg ccgtttgcaa tcaggtcgcc gtttacgtcg ccggttgcat
      601 cgtaacggat cgcgttggta tctacggtaa cgctgacagt gtattcgccg ttgccggtga
      661 tcggagcaac acctgcatca tagcacagat aatccgtatc gctgccggtg tactggagcc
      721 accactgacc gtctgccatg ctcagataag cctcgccagc ctgcggaacg acagcttctt
      781 cgaagtcgct ggtctgtacc tgctgcggag cagagctttc gtctgcggaa gcttcttcac
      841 tggattcctc tgcttcgctg gattcttcag cactggactc tgcttcgctg ctggagtcct
      901 tggagctgtc ggaagaaccg ccgcaggcgg tgaacgcagc agtcatcatt gcagatgcca
      961 gcaggaatgc caggatcttt ttggtcttga acatcataat tttcctcatt tccttcagtc
     1021 ggaaagtgca caggcacttt cgaacgttct tcaaaatgca gacagatcct gcaaaagcag
     1081 aggtctgctc agtactatga tactagattt ttcaccaaga tgcaagatgc tttttacaca
     1141 aatttcacct gataaaagac ctcacaattt gtgcaaattc ccagacattc tgcaaacaga
     1201 tccgctttat acnnnnnnnn nntcgccaga cgctctttgt gcggtattgc cctatataaa
     1261 aggtatccct gcgggcacaa aaaaacagca cgtgttgtgc acacatgctg ttggttctgg
     1321 agcgagcgac gagaatcgaa ctcgcaacct cagcttggga agctgatgtt ttgccactaa
     1381 actacgctcg catctgcctt tcggcagttc cgacaggcgg cagtgcctgt actttcgcgg
     1441 agcagaaaaa tggagctgga aacgtgactt gaacacgcga cctgtgcatt acgaatgcac
     1501 tgctctacca actgagctat tccagcaccg gttataccgt gagatctctc acgcaagatt
     1561 acctatttat tataccacaa attcttgatt tgtcaagagg tttcaaaaaa attctctgaa
     1621 aaatcaaaaa aacgattgaa atcgcagaaa agatgtgcta taataatgag gtactgtaaa
     1681 agtgctcgct gacaaatgat gcggcggaaa aaatttccgg ttcagacttt tgcacttttt
     1741 ctgcaaaaat gcaaaaaagc gattgaaatc gcagaaaaga tgtgttataa tagggttaaa
     1801 tcccatgact tcgtaaaaag gtaggtagca ttatgaaaaa gaaatccaaa tccattcttg
     1861 ccattgtact ggcgatcctc ctcgtgatcg tggtggcaat ctctctgacc acgaacattc
     1921 ttttttcccg aaaagacaca ccgaagattt tcgggcacta catctatctg atggaatatg
     1981 atactatgga agcagcggca ggggctgccg ttgccaccgg cacttccgga caggctgccg
     2041 gctcagtcga tgacaccagt gctgcggctt cgatccacca gcacactgcc gtgatcgctt
     2101 ccgcctataa agaaactgat ccgattgcca agaacaacgc tgttctgtgc gtactggctc
     2161 cggacgacgc ttccacggac atcgacgccg accgcgttgc tgtccgccgc atctacaaca
     2221 tcgaacagga cgaaaccggc gttctgaagt actatcccac gaccatgaaa gcgagtgatg
     2281 tgggaactga gccgcctgta actgtggaca gcattctcgg caagtgctcc tacgaaagcg
     2341 gcgaactgta tacatatgta aaattcgcca ccggcgttcc gggaatcctg atcctgatgg
     2401 ttctccccag catcattctg gtcatcatgc tgatcgtcac cattgtacgg gcaagcatgg
     2461 gacacgatga cgaatacact tttgaagaaa actatgacga agtttacagt gaagattcct
     2521 acgatgacgg cgattctgct tatgatgacg aagaactggg cggacgctct ccgctgtacc
     2581 gtccgtccga agcaacccag agccgttcct ttgagaagaa gcgttcttcc attgcggaga
     2641 acttcgaacg gaaacaggta aaccccaact cgccgtacca gaaggctcgt accatgcagt
     2701 tcaaggcaca gcatgatgtg ccgatctaca ccaatccggc agatcagctc ccgctggacg
     2761 gttctgcacc ggcagccggc agcaacacta ccgcctatga gggcacacat gctgcaagca
     2821 cttccaacac tgcaacattc ggcagctaca gcagcaacac ctacagcacc ccgccgatca
     2881 cagacgaaac acctgtcgca ccggcacagc cggatgctcc ggtttccact taccgcggca
     2941 gccacgaagc aagctctccg gaggaagcac ggaatctggg cgataccccg ttccgcagca
     3001 gccgtgcatc tgcaccggct cccgcaccga agcctgctgc accgtctgtt cccaagaaca
     3061 aggaagaccg cattgcaagt gcttctgtag acgaactgct ggcaatgatc gaagacgaaa
     3121 agaaaaagct gtaatcagtt acagcatcat cgaaaagaaa acggagaggg cgaaatgctc
     3181 tctccgtttt tttgtacagg aaacactgca taaagctcgt ctgacggctt tgcacgcaat
     3241 ctgcttgcag tgtttcctat attctgttct tttcggttat cccagttcgt cctccagcag
     3301 gatcatctgc tccatggttt cgtccatctg cacccgcagt tcctccatac gctggcactt
     3361 ttcctgcatc agctgatagt cgctgcaaac ggcttcgtcg gtcagttccg ctgtcagggt
     3421 gtccagttcc tcctgcatct tgtccagctg ctgttccaga cttcgcaggt cagcacgcat
     3481 tttcgcctgc tgggcacgct gtttcttgga acggtacgcc ggcttctccg ccggtttttc
     3541 cttgtcagca gccgatcttt ccgcaggggt ttcctccggt tccggctgtt tctgctgtgc
     3601 ctccagccac ttgctgaagc caccctgata ggtcgttgcc ccgtcggctt ttagttccag
     3661 aatgtggtct gccaccttgt ccagcaggta ccggtcatgg gacacaaaca gcagcgtccc
     3721 cgcaaaggac tgcatggctt cctcgatgac ttccttcatg gcaatgtcca ggtggttggt
     3781 gggttcgtcc agaatcagca cgttgccatg ctccagcatc atcaccgcga aacagacctt
     3841 tgcacgctcg ccgccggaga gtacgcccac cggcttgaac acgttttccc ctgtcaggcg
     3901 tacctgcccc agcagattcc gcacttccag atctgtccat gacggtacac ggtcatgaat
     3961 ttcctgcatc accgattttg tggggtgcag gttggtgctc tcctgttcga agtaggagat
     4021 cttgacattg ctggtccagc ggactttccc ctgatgcggt agcttgttct gcaagatttt
     4081 cagcagcgtg gacttgccaa taccgttatc tccgataatg ccccactttt cgccgcggcg
     4141 aacttcaaag gacacgtccg gcagcagcgt tttccgttct gtcccctcgc ccacggaaat
     4201 atcaacgtcc tgcactttca gcacttcctg cggcggatcc atttcatacg taaattccac
     4261 ccgtgcctgc ttgcgggaaa ccagcggctt ttcaatgcgt tccatttttt ccagctgctt
     4321 cacgcggctc tgggcggact ttgccgtcga agcacgggtg aggttgcggg caacgtagtc
     4381 ctccagcttg gcgatttcct tttgctgcat ctcgtactct ttccactgcc gtgccacagc
     4441 cgcttctttc agctgggtga aagtggtgta gttgcccttg taccgcgtca gtctgccccg
     4501 ctcgatctca cagacgctgg tgcacagccg gtcgaggaaa tagcggtcat gggataccag
     4561 cagcagtgct ccctgatagt ccttgaggta atcttccagc cacataacgg tcttgaaatc
     4621 caagtggttg gtaggctcgt ccagaatcag cagattcggt tcctccagca gcagccgtgc
     4681 aatggcaagc cgtgtttttt cgccgccgct gaaaccggag atgatgcggg aataggtttc
     4741 ctgtgtgaaa cccataccgt tcagcaccgt gcggatcttc acgtcgatct gatagccgcc
     4801 gttgttttcg aaccaggttt gcaggcggtg atattcctcc gcaccggcca tgtcaccgga
     4861 ctgcatggct tcttccagct ggtgcattcg ctcctgcgtt tccagcagcg gacgaaacac
     4921 gctccgcatc tcctcccaga cggtgctttc ccgatccagt ccgcccatct gttccagata
     4981 gccgatgctg gtcttggcgg atctggcaat ttcgccgtcc ccctccacca gatgatccgg
     5041 cagttcctgt tccgtgagga tccgcagcag cgtggacttg ccgcagccat ttacgcccac
     5101 aagcccgatg cggtcgtggt cttccaccgt cagtgaaacg tgggagagca cctgattgcc
     5161 gttataaaat ttattgatgt ctgtcatacg cagcagcata ctgttttctc ctttatccga
     5221 aaagttcgta atctttttta ttgtagcata tttccggaaa aattacaact ggttttttgg
     5281 aaacatctcg cggaaatcaa ttttcatgat agccccccct gaaaggggag ggggaccgcc
     5341 gttaggc
//
LOCUS       DAWBGR010000171         5344 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3783_length_5344_cov_3.758366, whole genome shotgun sequence.
ACCESSION   DAWBGR010000171 DAWBGR010000000
VERSION     DAWBGR010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3783_length_5344_cov_3.758366"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1237 a         1347 c         1487 g         1273 t
ORIGIN      
        1 cttacgcaca agctttgtgc ggtgttgcct tagcaacttt ctctattata caacattttt
       61 cgcagattga aaagagggta ttttggaaat ttttcaaaca ggacggaatt gccgttactt
      121 cgcactctgc cgccgttcca gttcttccag aatcatcttg ttcaccttgt tgacatcgcc
      181 gcagcccagt gtgatgacca gatcgccagc ctgtgcatgg tcacagatat agttggtgac
      241 ctgttcgaag ttctggtatt tccgttcgtc ggtggtttcc ggatcgcttt cgtcctgtgg
      301 gaaccagact gcgttgggaa ttttttcacc cagctgccgt gtgaagatgt gataggtgtt
      361 cttttcgcgg gagcccataa tgtccgtcag gacagcgtga tccggaatgg acagagcttc
      421 tgcgaattcg tccagcagca tggcggtgcg ggagaaagtg aacggctgga acactgccca
      481 gacctttcgg aacggcattg ccattgcagc tttcagggtc gcagtcagtt cggtggggtg
      541 gtgagcgtag tcgtctacca gcgtaatgcc gccgacttcg cccttcttct cgaaccgtct
      601 gccggcaccg cggaattctg ccagcccctt tgccacatct gcaaaggaaa cgcccacata
      661 catggcagcc gcagccgcag cagtagcgtt cagcacattg tgctcaccgg caacatgcag
      721 gacgatctcg cccagttctt tgccaaggtg catcagctgg aagtgggttt ccagaccgga
      781 aacgtggcgg atatttgcgg gataatagtc acagtccgca gttttgccga aggtgatgca
      841 ttccttgccg gtaatgccct ccatagcttt gcaggtgttg gcatcgctgc cgttgtagat
      901 aactgccttg gaggtcagct gtgcgaactt gtggaatgac ttgatgatat tttccacggt
      961 gccgaagtaa tccagatgat cggcatcgat gttcagcagc acggcgatgt ccggatagag
     1021 tttcaggaac gtatctacaa attcgcaggc ttcacaggtc atgatgtcgc tggagccgct
     1081 tctgccgctg ccgtgaatgc agggcagctt gccgccgatg aatgcggaca gatctacgcc
     1141 ggcggtgaac aggatctgcg tcaccataga ggtggtcgtc gtcttgccgt gggtgccggc
     1201 aacgcagatg gcattgtcat accagctggt cacaataccc agcagttcgc tccgttccag
     1261 caccggcacg ccgctggctt tggctgcaat cagttccgga ttgtctgcca tgatcgccgc
     1321 ggtgtgtacg atcaggtcag ccccctcaat attttccgca cgctgtccca gatatacggg
     1381 aatgcccatg tcgcggactg cctgtagggt ttccgtttcg ttgttgtcgg aaccggtgag
     1441 gtagaatcct ttggtgtgga ggatctgtgc cagcggatac atgccgctgc cgccgatgcc
     1501 gataaagtgg atatgctttt ttccgtttaa aatgtcaatg ttcaaaaaaa tcaccttttt
     1561 ctttcttgca attttcaaat ttatcgtgta tactgataag taacgtactt caaaggagaa
     1621 gcgtttgtgg tgacacggac gtttctcctt tttttgttac gaaattgaaa attcattttc
     1681 aatttctgcg gtgttcctct ggaaacggaa tttacctttg aagtcagcat ggtccgtttt
     1741 ccaaatgaca gaacggagcc ccgcagtatg tggaggaggt catcacatgg aaatcacaga
     1801 tattaaaatc agaagaatca ttaccgaagg acggcttcgg gcagttgttt ctgtcaccgt
     1861 agacaacatg ctcgccgtgc acgacatcaa ggtcgtacag ggggacgaac ggctgttcgt
     1921 ggcaatgccc agccggaaag acgaaaacgg tgtattccgc gatattgtgc accccatttc
     1981 tgccgccgcc agaaaaatga tggaacgaga gattctggag gcttatcacg agcatctcgc
     2041 gttaatggaa gccgaagcac aggcagaagc tgcggaggaa agtcctgagg cacagcctgc
     2101 tgcggtataa gttccggaat aacaatctaa gataccctga aagtcgtcgg agctgcctct
     2161 ccggcggctt ttttgttcaa gtcaacatca cacaagatgc ggtgcaatat ctgaaactta
     2221 ctgttccttt tgcagcaaca cgtaagtttt cagcagggca atctgtgttt tggcatcggg
     2281 gatctcgccg tccatgacca tttgcactgc ctgttccagc ggcagctccg tcacgtccag
     2341 aaattctccg tcgtccagat cctgtgcttc cggagccgag agctcctgtg ccagatacat
     2401 gtgaatgacc tctgtatcat atgccggtgt gggaaagagt tttcccagcg gagtatattt
     2461 tccgcaggtt ctgccggctt cctcccgcag ttcccgtctg ccgcagtctg ccggatcttc
     2521 gcccgcttcc agcttgccgg cggggatttc cagtgtgacc tgctgcatgg gatagcggaa
     2581 ctgctttacc atgaggacag tgcccttttc cgtcaggggt actacgcaga caccgccgga
     2641 gtggtgtacc acatcacgct gtacctcctt gccgtcctcc agttccgctg tgtcccgcgt
     2701 cacgtagaaa actttgccct gataggtctt ttcgcttccg gttgtttttt cctgtaagtg
     2761 catgttgtat acctccactt ttctgtttat gaagtttctt ctgtgccggt gttgtcttcg
     2821 gcaggcgttg tggtttgtgc ttccgtgttg tcatcaggtg cagtcagttc cggcaggatc
     2881 tcctcttctg cggaaaccgg atagtctgca cagcagacgc gttccgtttt gcggcggatt
     2941 cccattttcc agcagatcag catgtatgcg gcaaacagga caacgccgcc ggcagtcagt
     3001 gccgcaccag cgtgcagacc gggtgttgtg tagtggaaca caatggtatt gtcaccggct
     3061 tctgccgcta ccgccataaa gccgttgtcc acttcttcca cgtctgcagg cttgccgttg
     3121 actgtggctg tccagccctc gtcatagggg acgctgaaaa agaccagatt cgggctttcc
     3181 agcgaaatcg ttgcagtaaa gccgctggaa tcatagcgga acgaatcgca ggcagatgcg
     3241 gcacggttgc tgcattcctg caagtattct tcttccgtca tctgcacatt gttgttagaa
     3301 atgggctgca tatagctgcc gtatttttca atttgcttgt tggagagaac cagtgcacgc
     3361 atgagcaaat tggtgcggta agaagtagag gtgccgtccc acatgccctt ggaaaggtag
     3421 tagtcaaagg tgaagcccat cggcacatag gcggtatttt taaaccggta gaatccgttt
     3481 tctgtggtgt catagacaaa gcccggcagg tcgcaggtag gcgtgtcggc agtgatcttg
     3541 ccgatttcct gctgctggta gtaatattct acagaaagca gtccccgcag agcgtagtag
     3601 gaagtgtctg cacgggaggc aacatcacgg gtgataccca gttccttgta gaagtccatg
     3661 atcgatgccg gaacgacact gtggaacgtc cgcatgctgg gcagcttcca cagcatgggg
     3721 tagttgtcgc agttttccga aatgtcgatg cggaagaagt tgtcctctga tacagaaagg
     3781 gagatctcct gttccggatc gattgcctga tcgacgtaag tgcgggcacg gaaaggttca
     3841 aatgctccga aataaaccac tgccatggtg catgccacgc agaagccggt ggtcagagcc
     3901 attgcctgtt tgaggaatgg cttgtgcttt ttccggcagc ggagcaggta aatggaaacc
     3961 gccagaccgg cagtgcagat cagcagcacc aagtagaagt ggaacggata ttccgcaaag
     4021 gagaaccagt gaatttcatc gtcctccttg gtgggcagca gggagatcgc tgcaaatgcg
     4081 gcaaagaaga taaagcagat gcggaaaccg tatttccagt cgatctgccg gttgtccagg
     4141 ctgtaggcag tcatcagggc gagaatcaga atgggcatgt agaaccagcg tgcataatac
     4201 gaaccgttca gtgcatagaa tgcactgttc aggatcggta cacaggcaca gactgcacag
     4261 atcacagtaa tgcgttttgc ccagtgcccg tcacgctgct tgaggaatgc cagtacgcct
     4321 gccatggaga acatgggcag gtatccgccg atggaagccc attttccgaa gtcggaggag
     4381 aacaggttcg ggcgagccgg cacgtcggga atcaggaaca ggctcaacag gattcgccag
     4441 atacgggtgc ggtcgctgta agccaccatg tccatgccga acagatgctc ggaaacccga
     4501 ttgtttgcca gaattgccag agccgacggc agcagaatga cacaggcgat catcacgcca
     4561 atgactgcct ccagcagaac gaggaaaaac tttttcagcg atgcgtggaa attcttcgag
     4621 aagcaccgca cgatgaagta cagcaccaga aagaccactt gaccggtaaa gaaaaagtag
     4681 ttgatcgctg ccagcagtgc caccgaaagg gcaaacacgc cgcggcggtt ctggttgacc
     4741 agttcttcca tggcgatcag cagcaacgga aaaaacgcgg tcacgtcctg aaagtggttg
     4801 aagaaaatgt tgaacagctg gaagccggaa aaggcataca gcaggccgcc gatcatggca
     4861 gcgtgaggac tgcgtacaaa ccgccggata taggcgtatg ccgtcagaga ggcgatgccg
     4921 tgtttcatgc acagcagcac cggcatggca aatgttaccc agctgcgggg gagaatggtg
     4981 gtcagccaga aaaagggact gcccagcaga taaaaggcat aggaaccgat gaagttgaca
     5041 ccgaggtcgg tgtaccagtt ccagccgaag ctgccgctgc gaacggcatc atttgccaga
     5101 ttgtaaaagg ggatctgctg cgaattgtag tcgccatagt agatgtaata gccgccgtct
     5161 gcgatgatga tcggcagaat caccaccagc aggctgccga agcccagcag gaatgccagc
     5221 aaataccggc ggagttcctc ccgcagagca gggcggcgaa gtgtcactga tttcatgaaa
     5281 aaagctcctt tgtcagggaa gtgatgcacg gatttcattt tacggcatat gctaggtata
     5341 ggca
//
LOCUS       DAWBGR010000172         5334 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3790_length_5334_cov_4.335859, whole genome shotgun sequence.
ACCESSION   DAWBGR010000172 DAWBGR010000000
VERSION     DAWBGR010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3790_length_5334_cov_4.335859"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1320 a         1434 c         1311 g         1269 t
ORIGIN      
        1 acccccgacc tgctgattac aaatcagctg ctctaccaac tgagctacac cagcactcgt
       61 gtgctgcaac gataattatt atagcacaac ttcggggtac tgtcaagcat ttttttgagt
      121 tttttctttt tcaaagtttc ggaatgtgaa acttgtcttt ctaaatcaaa atatagctga
      181 ttttagctat acaggaatct gaaaataatt tttgtgggac aggcagtttg acagagagag
      241 cctcagctgc cggcacaatt caggatttat cggggcgatg tgggcatcgc cccctacttt
      301 ataaattagg cttccttgaa aaagacaccc aaactgagct gacagccgca agctgactga
      361 gagattttgc tgtgaaatct gcaattcaag aaaaaatttc accagaatcc ctccaccagc
      421 ttcgctggtc cccctccctt ttcaagggag gcttattcca aaataaaaag aacctgtttt
      481 gcatctgtgc ttatacagat gtaaaacagg ttctaaggga acaccgaata aagcttgtgc
      541 gtaagatgcg tcgtcagatt ttttggcaga ttgctcagaa attccgcagg cagactgtat
      601 gtctggcaag ggatttctgg gaaatctgca agcagattgc gtacaaagcc gtaaggcggg
      661 ctttattccg tgtttccgta aggaagaagt gggaatctgc cctcatggaa caggcagaat
      721 ccacatgaag atgtcaggat ttaaccaact gcttcgacag catcataatc gccaagttcc
      781 gcatcggaaa tatcgtattc gttgtaattt tcaacgctga acagttcctc agtctgggga
      841 tcatcgaaga acgaatcctt aataggaatt tgcagatcaa tcgcattgcc ctgatctgtc
      901 agaccgtcct ccgaccacag gtaaagttcc acataggact cccctgcctg catcggtcta
      961 tccagaatcg catactgcat gagcagctgt aaagattcgc tctggttcaa cggctggcag
     1021 tcagaagcat aggcatagtg accgctgtaa gccgaatcgg aagaaccgcc gtaaacattc
     1081 atgttgacgg aaacattctt atcactcttg gtcagttccg acagcattgc cagatcgtcc
     1141 ttttgtgccg catagagcat ttcgtagaat ttctttgtcg tttccggtgt cagattctga
     1201 tagatcatgc aatccagcgt gtatgatctt ccgtttttac ggaatgtgta atcgatgtcg
     1261 ctcacccatg tatcgtcagt actgccgttc tgcattgtct gatacagggt ttcaaagtct
     1321 gctgactgca attcctcccg ataggtctgc aacagctcct ctgcctgctc tgtactcaga
     1381 tagcaggaac ccacctggtc gcggaatgct gcagtgccct tgagttcctg cggcggattt
     1441 cgccatgcgt ccacatagtc cgcattggtc tgcaatgcat ccagcagctt ttccgtttcg
     1501 gaaacaggca catagatcag ccgacaaagt gtctggtctt tcgtgcggat aatcacgtga
     1561 taagcacaat aggaatagtc actttcggtg taatacttgc tctgataagc atcgctcccg
     1621 ccttcttccc acgttttcag gttttcgtcc agatagtagg acaccagtgt cacggcagtg
     1681 gggtcggtga tcgcaatgtc acggctcatc aggttgatat aatcccgata gttcagatag
     1741 ctgctgccgg aaatatagtc cgtgccgtct gtgctgcaaa atgacacgct ttcgatctcc
     1801 tgcattgccg gacgctgtgc cacgatgtgt ttttccaccg catgcatgcc cagcaggatc
     1861 cccacattca gcagtgccac aatgcccagc cccggcagta ccgtcagcag gtttttccac
     1921 ttcttcgtgg taatcagttc gtaagcaaag tacaccagtg ctgccaccag atagagcacc
     1981 aggaacgcaa accattcgtc ccgcatcata ccggtagtca tttcgctgaa catcacacaa
     2041 acgataaaga tacagatcac catggtcacc acaatgcggt agatgtgctg gagcagacgg
     2101 ctgggagcag actgtccggc agcctcactg cgacggcggt ggaacaagaa cgccgcgatc
     2161 gcaaaataca acagccccag caggaacgtg tacagatatg cctgccagct gggagaaaag
     2221 agagtttcta tgctcgttcc gtcgttttcg gacatggaaa agaccacgtt gacaccagag
     2281 aacaacaggt tattggcatt ccggaaaaat cccgttcccg cagaatccga cagaaacggc
     2341 agggcattca ccatagacga ctgcaacgcc agtacacaga gccgcggcaa aaaaagcagg
     2401 attccggcaa acagcaggtt cgtgaaaaca gtaccggtaa tgctcatgga aaccagcatg
     2461 ccggcaacca gcagaaaact catgcagaac aggctcgccg caaagggcag caggctgtcc
     2521 atcagcagag tgatatactt gtgtgtaaat ccacaggtga tgacagaagt cagcgtgccc
     2581 agcaccagca gcagcactac ccacagcagc acggctccgg cataactcaa gtacagggtg
     2641 atccgtttat ggggcaatgc gtgatacaag tctgatgccg cccgattatc cagaaagtgg
     2701 aacagcatca gcgtcatcag cggtgccgcc agcagcacca caagcagcgt cacattgatg
     2761 gcccacgctt catagacgac tttggtataa tgagggctgc tgcccaagct gatcgcctgg
     2821 gcaaccggct ccagaacggc acccagcacc acgatcacgg cactgaagat cccgatcatc
     2881 ttgcactgct gaaagccttc ccacagcagc cgcagggaaa agtgcatctt cttttgtttc
     2941 atctgataaa tcctcccctt gcgtgaaaat acgccacggc acaaaccgca gccagtccca
     3001 ataccaccag cagcagagcc agcgaaccca gcagctgccg gcgagcttcc tgccgatcct
     3061 gtcttccggc ataccgatgt tcaagatttt gcatgttcgc tctcctcctc taacaggtca
     3121 tcgatctgga acgtatagcc cagcgtttcc atttcatatg taaagacttc ttccagtgtc
     3181 agcggaagta cgtccagcag aatcggttcc attgcccgca gcttctgcac aatctcgtcg
     3241 cggtcgccgc tgacgatcag attggcaacg ctgccactct tctcatagtg ggaaacctga
     3301 atcccctgga acttgctctt gtcgtactcg ccgcggaacg cgatctggat cttgaactga
     3361 gaagttttca ggttctcgat gtcgctttcc agcaccaaac cgcccttatg gagcagtgcc
     3421 agctggtcac agatatcctc cagttcccgc agggagtggg aggtgatgat cgccgaggct
     3481 ccccgttcca gcacgtcctc gcagatcagc tttttcacaa agttccgcat taccggatcc
     3541 aatccgtcaa aggtttcgtc gaagaacaga taatccggac ggcaggacag tgccagaatg
     3601 gttgccgcct gccgccgcat gcccttggag aactgtccca gcgacttgtt ccgcggcagc
     3661 tgcaaatggc tgcggagttt ttcgaaacga tctctgtcaa aatccggata cagattccgg
     3721 tacatgcgtt ccatgcggtt cagatttgcc cctgccagaa aatagagttc gtccggcaca
     3781 taggcaatgc ggcgtttcac agcaggatta tcccagaccg gctcgccgtc gatgcggatt
     3841 tcaccgccgt ccggctgata cacaccggtg agcgtccgca agaacgtgga ttttccggca
     3901 ccattagaac cgaccatacc ataaatacag ccggagccga tggtacaggt gacattttcc
     3961 agtgcggtga aatcctgaaa ccgttttgtg agattcttga tctcgatcat gcctgactgc
     4021 cccctttctg caattctttc gcctcagtaa acgcctgctc cacacaggca gtgatctcct
     4081 ctttcgtcac accagccatt gccagtgccg tcagcatctc cgtcaggtct ttcatcttct
     4141 ctctcccttt ctccagcagc ttctgctggg catctgcaca gacatagtag ccttttccgg
     4201 gaacagactg gatcagccct cgggtgtcca gttcagtata ggctttcaga atcgttcgtg
     4261 gattgatcga attggtcatc gaaacgctcc gcaccgatgg gatctgtgcc tcaggctgta
     4321 acacgtccgt cagaataaac tgttccacct gatgaatgat ctgttcataa actggttccc
     4381 gcgacatcgc gtcgatctgg aacatggcaa aacctccatt ctcctgttat ttttcgggaa
     4441 gcactgcatg caatctgctt gcagtgtttc cttcacattc ccaagttcgt gttgcatgcg
     4501 ttttaggtgt tgtatctctc atgcaacacg ttaagtatac catatccgtt ctctcttgtc
     4561 aagaggtttt gcaaaaaaat ttgaggcaat accgcacaaa gagcgtctgg cgactttgtg
     4621 cggtattgcc ttgacaggca cgatatttgc cattgatgta taaatcttcc gattttgcag
     4681 acaatagctt tgcatcgcag cagcatgcga gattgtcggg gcagtgctgt cccgagcaag
     4741 gaggattttt gagatatgaa agcaactgga attgtccggc gaatcgacga tctggggcgt
     4801 gttgtgatcc cgaaggagat tcgccgcacc atgcggatcc gggagggcga cccgctggag
     4861 atctacacca gcagtgacgg tgaggtcatc ttcaagaagt actctcccat cggagaaatg
     4921 ggagaaagtg cggcgcaggt ggcagagatc atgcaccggc tggcagggtg tcccgtggca
     4981 gtgttcgacc gcgaccatgt cgtttcggta tccggagccg ccaaaaagga gtggaacgcc
     5041 cgacgggttt caccggaact ggaagaactt atggagcagc ggcggcagta cttttccgat
     5101 gccggcgaac cggaatttct gcccgcagag ggcatggaaa aggcggctgt ggcatgcatg
     5161 cccattctct ccgccggtga cgtgaccgga gccgtggcat ttctggaaaa cggcgagcac
     5221 gccgcactga gcgaaaccca gaagagtttg attcaggcgg cttcgcagtt tctggggaaa
     5281 caactggaag actaaaggca ataccgcaca aagattgtgc gacagatgcg tcgt
//
LOCUS       DAWBGR010000173         5303 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3824_length_5303_cov_4.427973, whole genome shotgun sequence.
ACCESSION   DAWBGR010000173 DAWBGR010000000
VERSION     DAWBGR010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3824_length_5303_cov_4.427973"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1522 a         1067 c         1259 g         1455 t
ORIGIN      
        1 caggtactga tgatacgatc atgtcgaccg catagaaaaa ccgccttcgg gcggaacata
       61 gggacataac aaactggtaa aagagtgctg ctgacgattg ctttccttag gaaacgctga
      121 acaaaagcgt agttaaggaa acactgaata aagcccgctt gacgacttta ttcagtgctt
      181 ctttataata atatccttgc tctcttcgat cagctcgccc cagtctctgt cgtttgcctt
      241 gaagttcacg aaaaaggaga gcgtatccgc cagctgatct gccagatcat acgggatctg
      301 ttcactggtc agcacctcat acagagatgc catatccaca ttgcctttct ggatcatatc
      361 gctgatggca gcatgcacgg ctgcctgctg cttgctgccc atattggggt tgtgagattc
      421 cacaatggac tcgatttttc ggatggtatc ttccccattg ccggagtcgc ccagtttgag
      481 caggtcaacg gggattccgt cctcttcgat gcggtggaag gtgatatgct tggcgatata
      541 cgcccggaca tcctgctctg ccgtttcacc gcagcccaca gacagctttt ccagtatctc
      601 ctcttctgta aaggattcac tggtatcaaa gacaataacg ggacggtgca gcttttgcag
      661 gcttaccatt cgttcggtga gacagtgtgt tttgccggag ccgcttttcc ccagtgcaaa
      721 gaagtgcata ttatccttgc gtttttcgtc aaagacctgg taggaaacac cgccgatgca
      781 gttttctgtg agggggataa aatgctctgg tgcaggtgct gtgctgtacc attctgcata
      841 gcggctttcc tcgatcatga gttccacgtc ggaatccaga atccggtcgc ttttcagggc
      901 aatcagagca ggccgcaggc ttctgtggtt ggaatacacc accagcggat aacggttttt
      961 cttggtggca tggagatatt cgcacgcttt caggcatttc agaatgcggc aggtgttgtc
     1021 ggcagttctc atagatggga tcagcacatt ctctatcgca gattcttcca ggatcagcag
     1081 cttgtccttg acgatcagct ggtttgtgcc ttcctggaag tccatttcct tgctgtgccg
     1141 gaggatcttg agcttgccgc tgcgaatggc atcattgagc gcagcaataa aatgctctga
     1201 caccgcatct tctgaagtgc cggaaactgt ctgagaatcg tacaaaatgg ggtagatcaa
     1261 tggggcgata ggctgactgt cgaaatcgag aatgcagtct atcacgcaaa agtaaacagt
     1321 atccagtaat gcaccaaccg caatgatttc tttgcttttg ccttggaaat agggactgta
     1381 ttgctcgaat tcagagttga agatatcgat aaagctttgt ttgaacctat ctcgaaacag
     1441 ttcgtagttt ttacagatgt gagatactat gatgtttacg agataattca agtcatcaca
     1501 catctccgcc tcttcttcct ttgtcatagc aagggcaaaa ttttccggca gcgtcagaca
     1561 gatctttttc tccggaggga gcaggtattg tgcatgggtg gagaaaatcg caagattatg
     1621 gggctgacaa tccatgctgt cattttccgt taggatgtgc tgcagcattt ccgttctttt
     1681 tttgtcatcg tcgataatgc tgcggtcgct caggataacg gtttcatcct ttgcgtggag
     1741 cagttccgtt ctcaccgcac gcttgttgga acagagggtc agcggctttg tgcctctgtt
     1801 gaagatctgc atgaggagcg tcgcaaatcg ttccgatgcg gcatcaggtg cagatatggt
     1861 cagaatacgc tccatgctgt atttctcttc ccggaaaaaa gaactcagca gaccgcagag
     1921 ggaaaaaacg aagagcatca tcttcttgtc tgtgtcaagg tacttttcgc agattccctt
     1981 tataccgaga gggcggtctt ttgtactggg aagaactttt ttggtgaaat agggggagca
     2041 gtgtttcagc aatttcatgt caaagcattt tctgttgcag tcaaatcgaa tagaggtgct
     2101 gttttcactc tgttcaatga aagaaaagcc ggcatattcc ggaaaatatt gcgtgctttt
     2161 tgggggaaaa cagcgaatgc agtacgcaat ataatcgttt gccatcgcct tgcttttgca
     2221 catccattga aaagattcaa acaggggcaa caggttcttg ctggctaatt tgtcacaagg
     2281 aacgactgca agatgggtgc tgtcatccat actggaatat gagacatata aaaactcttt
     2341 tcgttgtccg tctttgtcaa tatagcaatg gtgaatgctg tgaattctta gcttgccgat
     2401 tctttttatt tttctttttt cttgatctat gcagcttccg acacccgtta tttgatcttc
     2461 gccatatact ctgagaacct cctgaaatgg cattgccccg cttggcacag catgatttgc
     2521 agctacctgc atgtgattta tggcgatcaa ttgttctgcg gttgacattc cgtacattcc
     2581 gttcatatag gtgacctcct aaaaactttt tattgaaata cattttacct ggcacacggc
     2641 acaggcggtt ctgtatttcc aaaaagcatt atagcacatc ggaaaatttc atgcagacaa
     2701 aacgctctta tttttttgca ggcgctctgg gggaggggtg gaaaacgtaa aacgaacaaa
     2761 aaacacctgg cacacggcac agaaaaaaat caaaacccgt ttgtttttgt actccttttc
     2821 tgaaattgcc gtcggaaaag ttcctgtttg ggcgaaaatc aaacttctcc gatgcgtttt
     2881 tggaaaaagg ggaacaaaaa ttgtccggac ttttttcgga ggacgctctt gtgccgtgtg
     2941 ccgggtaaaa attatgcggg tgggagaatt ggggctgtgc cgtgtgccag gtaaaaaaat
     3001 tttggaaaag agatttatag tgctgccaag cagctgaaaa aagaacagtt tttttcaaat
     3061 cctcttttct ttccccggaa tatgtggtat gatatagata tcttgtaaaa caaaaggggt
     3121 tgcatccgta tggcatcgat ggaagaactg tataacacca tagcagacat atgggattct
     3181 tatgatatgt accggcagga acacgctgac aaatacggcg aggcagagga aagtccaagc
     3241 accatagaac agatcaagcg gcatttttat tatgtcgaaa agaacaaagc cggtggactg
     3301 atcccaaaga taaaaaataa gatcaacctg gatctggaac tcaaagaaac agacagcccg
     3361 gaggaaaggt cggacaagct ctggctgctg gaacagatct acgttgcaga aaagatcggc
     3421 gtatatttat attcgacgaa aaatgccgaa ctgccggaac gggagttttt gagccgatgc
     3481 aaggttctgg atattatcgc aaacccgaaa gggatgtatg tgtacaataa ttatgatgat
     3541 tacaacaatg aaattatgga ggcgtttgac acagtataca cgaatgtcca agcgtctgtg
     3601 aaaaatgcag atgaaattga gtggacggta gatgcgctat acagcggttg gtttcatatc
     3661 aatgcgtttt tctgtttagg tatttgtaca ggcaatgttt ttggggataa actctcagag
     3721 atgaaaaagc aaagagatgc tgttgaaaag attgcattcg atccgagtcg cattctttct
     3781 acaccaaaga atggaaaagg agtaaattcg gatacagcag aaacacaaga gccgcaaaca
     3841 tactccaatt tgaaagaacg tttctttatt atgctggtca ctgcgcaaaa aagagcggag
     3901 atcaacgata tgctcctcgg cacatttgac tatacaaagg aaaaagtgtt ggctgaccgt
     3961 gtgatggata taagagttct gaaggagttt ggaaagaacg aactctataa aaaagagatc
     4021 tcctttgagg agtttacaga gcttttgcgt tttggcagcg acgaagacaa actgtgcttg
     4081 tggcggggca ttacttcaaa gtacgctcct gagattccgg gaaaggaatt ctttcagagc
     4141 cggggtgaat tttccaagat cataaagtac atgaaaaaca tagaagagcc gattacatct
     4201 tctcatgatg tgaaaagagc ggttacccaa tggtgtttat tattcaagat cgtttatgat
     4261 ggtttgcagt ttgagcaaaa actgatctcc tacaactggg agggaaaaaa ggttctaaaa
     4321 actatgtcat cgatcttcaa gagctataat gcggacagaa atgtaaaatc attgtctgac
     4381 gatccccatt ccaggcgaaa ggaaagggaa gacagaaaag aaatgcattc tgctgcgatc
     4441 ctgcatcagt ttacctggga ctgtattgcg caaatgcaca gttcgccaga gatcacagac
     4501 tgcaaaaatg ccatcaaaaa gaaaatgctg ctggtgatgc aggagacttt ttcctgtaag
     4561 cttcagacgc tggacgatat cagaaaagcg aactacacaa tgcagtttgt ttttctgatg
     4621 gattggttta gtgatgcgtt tttggatgtg tgcgaaaaga tggtgcagga aaatttttgg
     4681 aagaacttca aaaattctgt aaatcaggtg ctgcatcaga agaatccgag cttagaacca
     4741 tatcgttctc cattgatcgt aaacagaaaa tatgagattt tatactgcaa tgatctggta
     4801 aaaagaagat tattaagcat tgcagcagca cattgtatca atccgaacat agtacagcaa
     4861 ataggaaaga ggttcgatgg catcgggcaa ataattgcac cgctttacga ttgtattacc
     4921 gaagaatcag atttatctaa aagtgtgaaa tatgaaagag agattgattt tgaaccattt
     4981 ccatatatga cagtgtccac aaaactttac atcaccatag accacttcaa caaaaccatc
     5041 accattgacg actgcatctt tgaagaacca tacgagcagg aactgcggca gtatctgtga
     5101 gtttttgccg gggagaaaat ttgaatcaat tggtattgac agctgcggtg taatagatta
     5161 taataaaatt atacattgga ctgcggctat attccaacaa tttatttgta aatatccgaa
     5221 atttagtcat tgatacggat ttctcataca aggaggcgat gcaaatgcag aaaaaaacca
     5281 ggcgatttga gagaacggcg gca
//
LOCUS       DAWBGR010000174         5272 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_3849_length_5272_cov_3.847230, whole genome shotgun sequence.
ACCESSION   DAWBGR010000174 DAWBGR010000000
VERSION     DAWBGR010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3849_length_5272_cov_3.847230"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1162 a         1561 c         1228 g         1321 t
ORIGIN      
        1 gtgcaaagcc gtcaggcgtg ctttattcag tgtttccttt acagtatcac ccttattttt
       61 ccccgtacag cagggcatca acgccctcct cgccggtacg gacacgaacc acatttgtca
      121 catcggaaac aaaaattttt ccgtcgccga tatgaccggt gtacagctct ttcttgacga
      181 attccagcag tccgtccagt tcctcagtgg gcagaacgat catgacaacc tctttcggca
      241 gcaggctgat ctccttgttc ttctcctcca cttcaaattc ctgtgtgccg tgctgcacac
      301 cgcagcccag cacctgatat accgtcatgc cggtgatgtg gaattttccc aacgcctttt
      361 tcagcggctc gatcttggaa ataccggtga tgatctcaac tctggataat ttcataacga
      421 attcctccca cgattacaaa aaatttccca aaaaagaaaa gatgccgaat ttccgcgaaa
      481 ttcgacatct ttgtctttct gtactctatt atagcagatg acacacctga aaaattgtaa
      541 aaatcagaac ctgcaaaata ctgcggtgtt gccttactga aaacggtcgt accgcagtgc
      601 ggacaagtcc actgctgttt cctgctccat tgccagctgt gacagtacca gtcccactgc
      661 cggcgagatg ccgaagccat gtccggtaaa gccgcatgcc agaatcagtc ccggcacttc
      721 ctcgaccttg ctgatgaccg gcacatggtc ggcacatgcg tccatccagc cagcccacgt
      781 ccggacgacc ttgatgtttg ccagatcagg gaaataatgc atgataccgc ggcaggctgc
      841 cgaagcggca atgctggacg acagcggctt acccggcttt gcatacggtt ccaagccgga
      901 gttcaagccg aatacgaaag agccatgctc agtctggtgt ccgtagaagt ctgcatctgc
      961 ggtacccagc atctgccaga acatcggacc tactgcttct gtgaccagtg tttccagcag
     1021 cacctgctgc atgggcacgt caatgcctac tgtggaggca ataaaacggc tctcatagcc
     1081 tgccgccaga atgatcttgt cgccttcata gacattgccg gacgctgtga cgacctgtct
     1141 tgccgcgcct ttgatcttcc gcagttccac aaccttttcg ccggagatga aatgtgctcc
     1201 caggcggcgt gccattttat aatagcccat ggtcgccatt agcgggttgg cgtgaccgtc
     1261 cgtgggacac cagcttgcac aggtgactgc ctcagacagg tacgggttga tgcgtctggc
     1321 ttcgtcgccg gtgatcatgc ggacatcgac accgtgtgca gcggcactgt ctgccagacc
     1381 tgtcaggatc ttctgatgct ggggcgtgct gcccagacgg agattgccct cctgataata
     1441 ttccacgttg gtatccagca tatccgaaag ctgcggccag atgtgctcaa tggagtacat
     1501 caccagcggc agttctctgg gatcacgtcc ggactggcga acaccgccgc cgttgcgggt
     1561 ggagccgccg ttgccgatat tttcgctgcc ctccagcaca aggacagaaa cgccattttt
     1621 tgcaaggtta tacgctgccg cacagccaat gatgccgccg ccaacaataa tgacttctgc
     1681 attcattttg tttcctcctt atcttttgcc agcactttca tttcgatcgg acgcatggga
     1741 gctctgccgg ttgccggtcc caactcgttg ggacgtacac ccagttcccg tgacacaatg
     1801 cctttcacca gtttggtaca ggtctgtccc tgacaaagcc ccataccagt tctcagatag
     1861 cgacggattt ccgtcaatgt aaacatgccg tcatgtacgg cacggcgaat ctcacctttg
     1921 gtaatttctt cacagcggca aacaatcata tcatcgtccg gctgcggtac gaagggtctc
     1981 agttcccggt ttctgtcatt catgctgtct cctcctcccg aaaaaatctt gctttcatgg
     2041 catattcttt cggtactttc atcgtaagca gattggtgtg gtcgaacgcc ttggcactcc
     2101 gcacatgaac cacctctgcg gcacagacct tttcaccctt tcggttcaga gccacgccct
     2161 tgtcgccctt ttgcggcagc ggcaggaact cataaggcag tgtcacttcc gccgtgccgt
     2221 cgccgcagtc ttcgttgacc aagaagatcg cctgaccgga acaggaagcc acgcacatgc
     2281 cgcagccggt gcatttcttc tccggatcaa tggacggcag tgccgtgatg ttgctgccga
     2341 tacggataca gtgcttcgga caagcgtcct gacacgggtt gcacggaata ttctgagtac
     2401 actcaatgac agggtgcaca ccggcactgt gtgtcacacc ggggaaccgt tcgatttctt
     2461 cctcagcaac gaaaccatgt ttcaacaggt tttgcgaaat ttccacacct tcttcggttt
     2521 cggtgatctg cttgccacgc atacccggag caaacatgcc ctgccgcagt ccctccaaag
     2581 cggcttcctg cttggcagct tcttccttac aggtttcctc cgaaatgaat cccagtcttg
     2641 ccgctgcggc aatgccggcg attctgccct cgatcatggc actggaagct tcttcaatac
     2701 cggacacgtc accggcagca tagatgccgg gaaccgatgt ttcaccgtac tcgttgacag
     2761 tgggaacaaa tccagccttt gccggattat cctccatggc acagcccgcc atatcgcaaa
     2821 gcttggacat cggcgaaagc ccgactgcca gacaaatcgt atccacatcg aaatgttttt
     2881 ccgtgcctgg aatgatctgc cagttcttgt ccacttctcc gatcgtcacg ccggtgacgc
     2941 aatcttcccc gtctgcggaa ataattgtat gggacagata aaacggtacg ccgcatcttg
     3001 ccacctttgc cgcatggaca ccatagccgc ctacgcggga agctgcatcg gcaacggcaa
     3061 ccacttcaca gccggcctgc atcagctgga agctgactac cagaccgacg ttgccggagc
     3121 ccagcatcag cacacgcttg cccggcaggg tgccgtgaag gttcatcatt gtctgtgcgg
     3181 cacctgctcc gataacgccc ggcagggtcc agcccggaaa tgtgaccatg ttctcgctgg
     3241 caccggtggc gatcagaatg gtatcgcctt tcacatgaat gacgtgatct tttaccttta
     3301 cggtaacttc cttttccgga aaaataccga tgacaatggc attcaggaca acctctacgc
     3361 ccagttctgc ggcttctgcc agaagttctt ccccgatgtg gaatccgcga atctttgcct
     3421 tgtgttcctt ggagccgaaa aacttatgga tctgtttgaa cagctgtccg ccgggcttgg
     3481 cattttcgtc gtaaacgaca acgtgcattc cctttttcgc ggcttcaatg gctgcggaca
     3541 atcctgccgg acctgcaccg acgacgatca tatcataacg cttcatcatt ttgctgcacc
     3601 tcccggctgt tctgctgtca caccatattg tgtccgaatc tccatgcctt cttccaaagg
     3661 cgtaatacat gtccgcacat tcggctgccc gtttacgacc attacgcagt ctgtacagcg
     3721 accgatcgca cagaagattc ctctcggttt gtgttccttt cgggtatagc ggtgtaccat
     3781 cacgccggca tttttcagtg ccattgcaat gggctctccg gcatatccct ccaaaggctt
     3841 tccgtcaaaa gtaaaggaaa ccagctttcc cttttcatat gtccccagaa tcgggtgttc
     3901 ctgaattctc atgtctgctc ctcctgtctg atcataccat ttgcggggta tcccaaagct
     3961 tcagctttgt accgatgcct tttttcagtg cattgcggta aaccatagtg ccccaggcta
     4021 catcttccac cggcatgccg ccaatggaat acacaataat ttcgtcctct ttccgatgca
     4081 gcggaatgtt gcccatcaga atatcgccaa gatcatcgac ctgctccggc ttcatcttgc
     4141 cctctgcgat cagatccatg acatgcaccg ccggaatcgg cacggtgttg taggcattcg
     4201 gtttcatttc ttcttcccat gcctcgtaca gcttgatgtt gtccgttaca gttctcgcac
     4261 ggtgaattat gaagtcatca tcaaaccgga gagccgccgt ggaactgatc agtgcacccg
     4321 gcttgatcca ttcctctttg atgtacggat acaggctggg gtcaccggta ggggtggaag
     4381 tacttgccga aacaatgtcc gcatttcgga ctgcttcttc tatggtatcc acagcgtaca
     4441 catttacctg cggatattct tttttcacgt gggcaatgaa cttttccagt gaagccttgc
     4501 cgcgtccctt gaccttaatg gtttcgatgt tcggacggac tgccatgatc gccgccagtg
     4561 cggtcttgct catcacgccc ggaccgataa tggcaaccac cttggaatcc ggcctggaca
     4621 agtgcttata tcctaccccc ggcactgcac cagtccggta tgcggaaaga atatttgcgg
     4681 acatgtatgc caccggtgca ccagtatcct tgtcgttcag cgtcagcatc agaatggaac
     4741 ggggcaaacc cttgtccttg ttggcacagt tggagccgta ccacttcatg cctgccatat
     4801 caaacacgcc gcccagataa gccggcattg ccatgaatct tctgtccggt ccatctaccg
     4861 gcatttccgg aaacggagaa gacggcggga acatgaccat acaaccgtgg gagttcccgt
     4921 tcgcaccgcc catgcggtag tttcccacct tcatcagctt gaacatttcc tccatggctt
     4981 cgatacaacc tgccatatcc ttgacaccgg ctgccaccat gtcctcctca ttcagataca
     5041 aaaaatcaac ttctggatag ctcatctgaa atacctcctc cgcaacgcat cacgcacgac
     5101 gctgtacgct gctttcctga aaaataaaaa ggagttcaga tgactgattc atctgaactc
     5161 ctttgttcga gtattatctt atcacaatta cgggaggaaa aattgtaaaa aacggaacaa
     5221 gggcaatacc gcacaaagat tgtgcgacag atgcgtcgtc aaatttttcg tc
//
LOCUS       DAWBGR010000175         4964 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4092_length_4964_cov_4.794459, whole genome shotgun sequence.
ACCESSION   DAWBGR010000175 DAWBGR010000000
VERSION     DAWBGR010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4092_length_4964_cov_4.794459"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1590 a         1003 c         1111 g         1260 t
ORIGIN      
        1 cctttctaac cgtatatttt tatggtttag ataaaaaagt gattctcgtt gtacaaaatg
       61 cacaataaaa aaccagagat ttatcattgt ttgtgtaaaa gtgttgcaag aataattgag
      121 ttctgttata atacacttac agctcaagca gctgacaact ttaaaattga ataaaggagg
      181 aaatcaagat gaatgaaaca aattctcatt taccccctgt gcagggtgga aacggattgt
      241 ttgtaccaat cacacttgaa gagatggcaa aaagtcctga aatttttgga agagcaggtg
      301 cagcgtattc agaagtggca ggaactccac ctgtcgttcc tcaagaactg caaaacgctg
      361 tgactgagcc aaaagtccag agactgaatt gtgcaaatgc tagtgagata ttttatctgg
      421 tggtaaacat actcaatcag caaaagcctt tatggcggta ccaaaaccac ttttacatga
      481 aggagaacaa tgtatatgtt ccttacaata agaaggaatt catatgcaga gtgtattctg
      541 atgttacagc gcttggctat gagttcacac agcctgccat ttgcagtatt acaaatgagt
      601 tggaaatgcg agctgatctg taccaaggag agccaaatga cgaacgctat acctattgtt
      661 cgaacggctt tctgaataac cgtacaggat tgtttgatat tgtttctgat tactttccga
      721 ccattcagct taccggggcg tatctgggat ctggcccgca gtatcatcct aacatggacc
      781 gtttcatgtc aattctgttt gctggagata agatgcttga agagagagca taccagatca
      841 ttggctactg catcagcagt gacgctcatg cgaaacgctt ttttgttttc cctggtgctt
      901 ctggagataa cggtaaatct acctttatcc acctgatacg cagtttgcta agtccttctg
      961 cagtcaaagg cttatccatg aaaaatctgc ttggcggcaa ttttgcaatg tcagagattc
     1021 aggaggcacg gatcaacgtt agctctgatg aaggtgcctt agaactcaat acagatcagc
     1081 tggcaaagct aaagagcatc tctggacatg accggcttac ggttgacaag aaaaattcga
     1141 atcaggtcag ctttacgtct acttgtaagg tcattatcgc aagtaatcac aatattggta
     1201 tggcgtacag cagcagtgac agcgccattg ttcgcagaat ctgcacactg ccatttgatg
     1261 tgaagatccc gaaaagtcag caaaatccga acattgtttc agagatactg gggtctgaat
     1321 tgaatgcagt cacaacagag gctttgaatg cgtttttcag actgaaagca aatggctatc
     1381 actttgccgg tgacgatgcc gggctggatg actacgctct gcaattcacg ccgatcaatg
     1441 cgcaatatca caacatagaa cgttttgtgc agtgtcatgt gagatctatg cccggtaatt
     1501 tcgtgctgac aaaagacttg tacgatgttt tccagaattg tctcgacttt aactgtgatg
     1561 tattcaaaga tgtgacgggt ttttcacagg cattaaacaa ttaccttctc tcgaatggat
     1621 acgttgtaaa aaaagtcaaa aagcatcagg gaaacggata tgagggcata gccatttcta
     1681 atccctaaac cgaaaggagt aaaatgttat gataacgatt aaagacttga aatcaaaagc
     1741 atcttcaatg gagtccgacg gtgtgtatat catgccgttc aatcctaagt atgaaaaaga
     1801 tgaacttgaa gaaagaatgc tgaaagtagt agaggagcaa ccgagcgttg ctcttcggtt
     1861 cttatgtttg attggcgatt ataaagttgg cacctcaaag cagtatatca gtcagatcat
     1921 tgactcgaac gatattccaa gcttgggaga gtcaatgctt atcgcctctc caactggttc
     1981 tggcaagaca agcgcagtaa tcaagatgat caagcacact tcaatgccgg tcatctatgt
     2041 gaccaacaga aagatgacgc tttgtcagtt caaaaaggac tatatcaagg catcgaaagg
     2101 actcgatgtt cctgcagagt tgcttgacag catttccttg ggagagaaca tcatcgccat
     2161 tacctatcaa gaacttgctg agacaacgta taagtataaa ggcaaaaaat acctcctcat
     2221 acttgacgaa gtccattgtc tgctggaaga tgctaatttc tcagtgtacg cagagaaaat
     2281 tattcggtat ctgaaagcaa atcgtgacaa taccgcaaga atttacctca cggcaacacc
     2341 agatgcggtc attcctgtga tagcggaaat agaatgtgaa tcaggacaag agcaggcgct
     2401 ttttgcaatg gactgggata ccaatgtaaa atctgttttc catgcgtatg cttcatacaa
     2461 aacaagattg aagatggttt actcgatgga aagcaattgg aactatatca acttcaagct
     2521 atacacccca gacgacacaa aggaacttgc tgattatatc aaacgagaaa atgagcaggg
     2581 caccaaaagc ctgatctatg taaatgacat cagcaaggga aaagtgcttc aagaagtgtt
     2641 aggcaatacc cagcacatct actcagatga agacaaacgt gcagaaattg cagagatcgc
     2701 tcaaaatgag agattttcag atcgaaacct gatcaccacc aaggtggctg agaacggagt
     2761 gtccttgcac gacgatgaat tgaatctgat tgttgtcgaa acactggatc caatcacact
     2821 aaagcaggtg attggcagag cgagagtcaa ccgcaagaat cccagagaga tcacggttct
     2881 tattcttgac tactctatga ccgacatcgg caatgcgatc gcaagtgcag agcagcaaat
     2941 gagaatggtc aaagaggtca aagcagatcc agatctggca ttggagtatg cagcgaatta
     3001 tcctgcactc gtatattacg acagcaaagt aaagcaccct gttgtaaata caatggcaga
     3061 aaaggcactt gcgtatcaga tcgactttct cagaggcttg cgaaaagacc ccgaaaagcc
     3121 tcatgccttt gcccaggctg tccttgaact ctatggtaaa aagccagtca tctcagacga
     3181 tatgttcctg tactatgaca gaatccttga cttcaagaca aaggctgcgg aggcttttga
     3241 tacatacaag aactctgaga agaacgccga gtgccttgac agcctcaaag caaagctgaa
     3301 ggttgcctgc aacagcacca aggtttacaa tgatggcaaa cagctcacca gcaacattca
     3361 aatcagtacg gttaatgaca tactgaaggc agcaggaatt gcgtacgaaa taaaggcaaa
     3421 agttgaagtt ttcaacatag cctctgtcta attataccat tacctataag cacttgtcaa
     3481 tagaggagaa tgatttttct attgacaggc gctattattt cagttgtata ctatagtcat
     3541 gacagaataa tctggctata tcctaaaagg aggaatttaa catggaaaca cctaaattta
     3601 ctggacgaaa cgtccctatt accgaaatcg caaaagcaac cggcaagagc cagcagttta
     3661 tacgcattgg cattcagaaa cgtatccttc aatttggata tgccctcaaa caagaaggat
     3721 ccagcgaata caattacttc tgcccagaca aaaaggtatg ggaggagacc ggctatttta
     3781 aggatatgag caataacgac gtagtataaa gttgtttaag gcaaagaagg gagatgatgc
     3841 caatgaaact gggaaacggc gttggtacag tttataagct gtccggcaaa agacgtaatc
     3901 cgtacatcgt acgcaaaaca gtcggttggg agatcgatgt ggaaaccggc aagtgcaaac
     3961 aagtctgcgt taccatcggt tatgctccga caagagcaaa aggtcttgag atgcttatgg
     4021 agtacaacaa aaatccatat gacattgcgg tatcaaagat cactttttct gaggtgttcg
     4081 acaaatgggc atctgagaaa tttccgacga tctcggaatc gaatgtaaag ggctatcagg
     4141 catcctataa ggcttgtcag ccgctgcata accgagtgtt caaggatctg aaactgatgg
     4201 atctgcagag cgttgtagac acctgcggaa agaattatcc cacacttcgt aaattgaagg
     4261 tgctgctgaa ccagatgtac gactatgcca tgaagtacga gctttgcagc aaggactatt
     4321 cacagtacgt gaatatccag aaattcaaga acaagaatcc aaacaaaatg gatcgcacgc
     4381 cgttcacaga aggcgagatc caggctcttt ggatgcagaa ggacgatatc tatgcgcaga
     4441 tcgtactgat gctgatctac agcggtgtgc gtgtgtcaga actgctcgac ttgaaacgtg
     4501 aggatgttca tatagacgag cattgtttca acgttgtgcg tagcaagacc agtagtggca
     4561 tcagaatggt tccgatacat gacaagacct atcccatatt ccaaaagtgg tatgaagagg
     4621 gctgtgagta tttgctgcac acacccgatg gaggtcactt tacatatcgt aactactatg
     4681 attcctattg gactcctatc atgaaacgca tcggatgcac gcacaagccc cacgatacac
     4741 gtcatacttg tatttctatg atggcagcaa agaatgtcaa tcctacactg ataaagaaga
     4801 ttgtaggaca ttcaggcgca atgtcggtta ctgaaaaggt ctatacacac gtaaatgtgg
     4861 aagagctttt ggaggcgatc aataagatat aagaaaaagc tgggacttcg gtctcagctt
     4921 tttttgtaat gtagttgtta ttcaaagctc tctataaaaa atct
//
LOCUS       DAWBGR010000176         4938 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4123_length_4938_cov_3.243293, whole genome shotgun sequence.
ACCESSION   DAWBGR010000176 DAWBGR010000000
VERSION     DAWBGR010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4123_length_4938_cov_3.243293"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3611..3620
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1727 a          985 c          996 g         1220 t
ORIGIN      
        1 gtattatgat atgcaaggag gtattaataa tcatgaaaaa gaatcaaaaa gacattttgc
       61 tacaagattg gcttgatacg tggtatgaga tttacgcaaa accgttcgta aaaaaatcaa
      121 cactggtttc ttacgaatgc tacattcgtc ttcatatcaa gccatatatc ggaaatatca
      181 aattgtgtga ttttaacacg atgacatttc aacgcttttt caatcaacag tttcaaaacg
      241 gcagctgcaa agggacagca ctttctccca aaaccatcta taatctgcga atgatgcttc
      301 atgaagcaat cggcgatgcc gtaaagaatg acttaatgaa gtgcaactat atagaatacg
      361 ttaaacttcc aaagcttcaa aaagaagatc agcgtgttct tacgaaaaag gaacaagcaa
      421 agcttcttac aacactgaag gcaagcgatg atccgtttgc tttcggtgtt tttttctgtc
      481 ttgctacagg aatacgtttg ggagagttgt gcggtttacg ctggagcgac ttttcagaac
      541 acaacggaaa agtaactgta cacattcaga gaactttaaa ccgtattccg gacttggaaa
      601 aaggcacggg aacgattatt aacatttcag ctccaaagac aaaagctgca aacagaatca
      661 tcccattgaa tcaaactgtg gttgaattct tgaaaaagca ttggatgaaa cagacagagt
      721 tacttggaaa agaatatgct acaggagatc actatgttct tagccgtatt ccaaacaaac
      781 cagtagaacc aaaattcatg cagatgcgtt tcaaacgttt tctatgcgag tctcatataa
      841 ctgatgcaaa cttccatgca ttgcgacaca ctttcgctac aagggctctg gaagcaaatg
      901 tagattataa gacacttagt gtacttcttg gacacgctaa tgtcactaca acaatgaatt
      961 tgtatcagca tattctatta gatcaaaaaa tcgttgcaat ggaaaaaatc ctacagcaat
     1021 tttgatactg aaaacgaaat aaatattttt cgtaaagctc atgccgttgg cgtgagcttt
     1081 tttattgccc aaaggaggtt tttaatctat gaacgctgaa aaaaagagtg attggtattt
     1141 ggcaagaaaa aagatgctgc gtcatgaaaa atatctcata gaaaaagatc aagaacatgg
     1201 catctttctt tcaggtgata cagagattgc cgcaaacatt gacgtttata aagcagtaga
     1261 acgaaatatg atgttcgcaa aattacgaaa aaccattcag cagctcaatg aaaaagaaca
     1321 gtatctaatc acttgcattt attttcgtga tatgacaatt cgccagtatg cgaaagaaca
     1381 acacattgcc tatgcaactg catgggaacg tcatgataag ctcttgaaaa agatgaaaaa
     1441 cgtaatgatt tctgtgtaaa acggcacatc catacaaaca cgcatatgaa aaaccttatt
     1501 ttacaaaaat atgaaattgc aaaaaggcaa aaggagtgtt ttacttgcaa aagataattt
     1561 ctcatggctt tatcgcattg aaaccagaaa aaatttctga actgtcactg gaagcatatg
     1621 gcgttctttc catgatggta aacgaccctc aatgtgactt cataaccctt caggaacttt
     1681 gtgaactttc tccaaaagac agcaaatcga tcttgaaaag catcttggag gaactcgtaa
     1741 ataaaaactg ggttttcgaa acagtcgata acaaatttat ggtaaacaaa gaaaaaatga
     1801 ttatgaatat gacttacgta ggtgcaacga tcaatagggg gtgatgatat tgccgattgt
     1861 acgaaaaaag gtgaaatgtg acttcacaac agtacacaat gcatttcttc gagacaacaa
     1921 acttggggca actgccagag gagtactgct aacaatgatt tccatgccgg aaaactggaa
     1981 attttcaatc aagggattat ctgctattct tcccgacggg gaacgtaaaa tcagcactgc
     2041 tctaaaagag ctggaaaaca gaggatattt aatacgcaag cgaatttatg taaacggaaa
     2101 aatcgcagaa tggatttatc ttttcagcga tgagcccatg gaggaagacg aagaaccgga
     2161 cctgccacca gatctgactg aaacagaacc acccactaag ggcgatacgc agcatcctca
     2221 agcttcaaaa aaggacgata taccagccgc cgaagatgta caaaaccttg atctacaaaa
     2281 cgtcgatcaa gaaaatgtag atcaagaaaa cagcgtctat aatcaaatac tatataatca
     2341 aagactatat aatcaagtat tatataatca atatcctatc catcacagta atacgttaac
     2401 ttgtatcagc ggctctgatc ggatagatgc ggatagattt gaaatcacac agcagtctgt
     2461 actagagcaa atagaggctg aggcattgct gtctgaaaaa aaccgtaaga caggaatgct
     2521 cttgtacgat gctgaaacaa tcagggaatt ggtgaattgc atcacatggg tttacgttac
     2581 gcctaaaaca actctgccta tttgcggaga caacctgagc acggacattg tccgaaagga
     2641 atttcagaaa ttaacctgcg aacatatcgc ctacgtactg gactgcattg cttctaccgg
     2701 agctgctatc aaaaaccgcc gaaattacct gttaacgtgc ctttacaacg ctcccaccag
     2761 catggcaggc tattatcgca atcttgccca acacgatttt gcaacacaac acggtacgga
     2821 aaataccgaa actgacaaga gcccctatgc ctatgggcaa tttatagcaa atttataatt
     2881 tcacaggagg attcacatga acatgaccat tttgacacag aacgacgtat tcctgaacta
     2941 cgaccatatc atcgcaatcc gagtttattc cggagaagta tcgctggata acgggaaaac
     3001 tatcgctgca tatcaggtcg ttgcagattt atctatgcca attttgaatc tggacgaaac
     3061 agaaaacgca caggaagccc cacagacgca gattgagctg ggggtatact tcgacgaggg
     3121 agaatgccag aacgctgcca gagcgctgtc agaatggctt aataacgctg atagcagacc
     3181 gcatttgttc aaaatgccaa tggcaaattc tatttccgga tattctgaca cgaatgctgc
     3241 cgcttctaaa ctggaatatc gaccggatga agacgaagct taaaacttgc atatagccaa
     3301 tacaggttac tttgcgaagt cggaggtgaa aacatatgcc agacggaaca gaaaaagatg
     3361 tccgaagtgt cgtcgatctt gtattgcaat gctcagacat caccaaagag ctagtacagc
     3421 aagcaatgcg tgactatttg cagcaaaacc aagataaaag cacacatgga aagacaagtc
     3481 ttcggaaatt agccgaacaa agcaacggaa atttagattc catcgaagtt tcttctgcaa
     3541 atttgcagga tttcaaggaa attgcaaaaa aatatgacat aacatatgcc ataaaacaag
     3601 attccagcac nnnnnnnnnn cagcactacc ccacccgcaa accatgtgtt ttttgcgaca
     3661 aatgacacgg ataatttcaa gcgtgctttc acggagtacg cagccgaagt atcagaaaaa
     3721 gcgaaaacac ggtatgagat accacgagaa cagttcaaag cacgtgctgc taagatcgaa
     3781 aaagagccac gccaaaaaga acaggttcga accagagaaa aacaagaaac gagatgagga
     3841 acatggatac ccagaaagtg aaaaaaggaa ttctttttgc aattccaacc atcatgtttg
     3901 gctatgccgg agacaagcta gcatttgcat ggagaattac agaaggcaca aatatttccg
     3961 aaaaactttt cccgtttttc acgaatttgg gtgtttcttt tgcgaatcct gcacccagtc
     4021 tgcacccgat agacttgttc attgggctgg cattgggcat tttaattcaa gtcatcatgc
     4081 atttcaagcg taaaaatgct aagaaattca gacatggcga ggaatatggt gctgcacgtt
     4141 ggggtacgcc aaaggacatt gaaccgtata tggacaaatc caattttgag aataatatta
     4201 ttttgactca aacagaacgg ctgacaatgg gaaaaccatc tagtcctaag tatgccagaa
     4261 acaaaaacgt tcttgtaatc ggcggctccg gctctggtaa aacacgattt ttcctaaagc
     4321 ccaatctcat gcaaatgcac agcagctacg ttgtaactga ccccaaaggc actgttttgg
     4381 tagaatgcgg aaaaatgtta cagcgaaaca aataccagat tcgtgttttc aatactgtgg
     4441 atttcaaaaa atccatgcat tacaatccct ttgcgtatat tcgccccgaa acaagagagc
     4501 aagacattct gaaatttgtc gaagttctca tcaaaaacac ttctggtagc gaacaaaagg
     4561 gcgaagattt ctggacgaaa gcagaacgac tgctttacac cgcctacatc gccatgatgt
     4621 atacggtttc cccaccagaa gaatggaact ttgcaacgct aattgacatg atcaactgtt
     4681 ccgagtgtcg ggaagaagat gaaacgtttc aaaatgcgat tgacattcag ttctccatgg
     4741 tggaaaagtg gttgaatcaa cagctggatg aggaagaaaa tcttggcgaa tttgaagcaa
     4801 tgcgtgaaat atcaccgaca aaagagcaaa tgtcaatcgg aagctttgca gtaaagcaat
     4861 acaaagcata taaactggct gctggtgttg tatgctctaa aagacttctt aatcaagcag
     4921 ttgggaagtc tcttagaa
//
LOCUS       DAWBGR010000177         4859 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4194_length_4859_cov_5.416320, whole genome shotgun sequence.
ACCESSION   DAWBGR010000177 DAWBGR010000000
VERSION     DAWBGR010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4194_length_4859_cov_5.416320"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1237 a         1183 c         1398 g         1041 t
ORIGIN      
        1 tgacgaaaaa tttgacgacg catctgtcgc acaatctttg tgcggtattg cccagggtgt
       61 cctttttcca tgcagcccgt tttttctgaa aaggcggttt gcagaacagg cggaggagaa
      121 ttctgccgtc atgcggcggc aatgtgcctg ctgaacaggc ggcagtctgt gcgagtgccg
      181 attcggcagg cagactgaca gtgatttcgg aaaagagaac atttgtgctg tccccacgtg
      241 agaaagtgag gatatttatt tggcaaacga aaaagaacag gtaagagaag tacgaaacaa
      301 tccccagccc cgcaagagaa gctatggggc aagaccgcgg cagcagggtg ccggcgagcg
      361 tccctccggc ggcaatcgcc gtcacaacaa caatccacgc cccagaaaca acaacaatgc
      421 acagccgaca cggcagaacc gccagaagcg gacaccggta cgtatcattc cgctgggtgg
      481 tctgaacgag atcggaaaga acatgaccgt gtttgagtgc tgcaatgaca tgttcattct
      541 ggactgtggt ctggcatttc cggatatgga tatgcctggt gtggatattg tcattccgga
      601 tttcacctat gtggagggca acaaggacaa gattcgcggc atcgtcatta cccacggtca
      661 cgaggaccat atcggcggtc tggcatatct gctgaaaaag atcaatgtgc cgatttatgg
      721 cacccgcatg acactgggac tgatcgaggg caagctggaa gaacacgggc tgctcaagca
      781 ggcgaagctg atcgaagtgg aaccccgcac caccgtgaga atgggctgca tggcagtgga
      841 attcatcaag gtgaaccact ctatccccgg tgcagtcggc atggcgatcc atacaccggc
      901 tggcatcatt gttcacaccg gtgactttaa ggtggacttc tcgccgatcg acgatgagat
      961 catcgatctt gcacggttcg gcgagctggg cagccgcggc gtactggcac tgatggcaga
     1021 ctccaccaat gcagagcgta ccggatttac atgctcggaa cgcaccgttg gcggcagctt
     1081 tgaaaagctg ttcaagcggg cggagggaaa gcgaatcatt atcgctacat tctcctccaa
     1141 tgtgcaccgt gtacagcaga tcatcgatca ggcaaagaat tacaatcgaa aagtggcggt
     1201 gttcggccgc agcatggtgc atgtcatcga aaaggcgacg gaactgggct atctgaaagt
     1261 gcccaagggc attatgatcg atattgatga aatgaaccgt tatccggacg accagatcgt
     1321 actggtgact accggcagtc agggcgaacc gctgtcggct ctgacacgta tggcaatgaa
     1381 cagccataaa aaggtgacga tcacaccgaa tgactttatc atcatttccg cgaagccgat
     1441 tccgggcaac gaaaaatatg tcacacgggt agtcaatgaa ctgatgaaat ccggtgcgga
     1501 agttatctac gaatccatgt atgaagtgca tgtttccggt cacgcgtgtc aggaggagct
     1561 gaagctgatc caggctctga cgaagccgaa gttcttcatt ccggtgcacg gcgaatataa
     1621 gcatctggtc aagcatgcgg aaattgcaaa gtctatgggc atgcccagcg atcatatcat
     1681 catttccaag atcggtgatg tcatcgaaac cgacggcaac tccatgcgta tcgtttcaca
     1741 ggtgcctgcc ggtcgggtta tggtagacgg tctgggtgtc ggcgatgtcg gttctatcgt
     1801 gctgcgggac agaaagcatc tgtcacagga cggtctgatc attgtagtga tcggcatcga
     1861 acgggcagcc aatgccgtag tggcaggacc ggacattatc tcccgcggtt ttgtctatgt
     1921 gcgggagtcg gaagaactca tggacgaggc acgcaatgtc atgacgactg tgctggacaa
     1981 ctgcaacgca cgggacctca aggaatgggg aacgctgaaa accaagctgc gtgatgtcct
     2041 gtccgacttc atctatgcca agaccaaacg cagcccgatg atcctgccga tcatcatgga
     2101 ggtataaatg cctgttacag gctcttgaag atagctgttg ctgaaaagcg gctgtctgca
     2161 aaggagaatg cagcatgaaa cgaaaatatg gggtgatgat cgccacatgg atcttgtggt
     2221 ttatcagcgt ccttgtaccg ctgttttgta tggtgctcca gcgagtgccc cagcggtttt
     2281 tgaccataca atttctggtg atcttgctgt tggggattct gctctgtgtc cagtggatct
     2341 ggctgcggcg tgcagaacgg atacagaata ccgctgtccg cggcgagatc gagggtgccg
     2401 gcatggctgc cctccagagc atgactgtgc cgatgattct ggcggaacgg gacggaaaga
     2461 tcctctggag caatgagata ttccagaacc gcttcgtgtc gggacgctct gtgctggggc
     2521 gttctgtgca ggacgtgatt catctgaatc tggcggctct ggatcaaaac cgtgacctgt
     2581 taatggagca caacgggctg agctatcggg tgcatgctgt ttccagtgcc cagaaccagc
     2641 aggaaatcat tgcggtgcag tttctggaaa tcacagatat tctgcggttg cagcacgagc
     2701 agaccaactc ccgtccctgc gtaatgctgc tggtgatcga cagctatgac gatctgctgc
     2761 aatacgccag agaaagcgaa aaggcacagg tttccgccga ggtggaacgg gtgctggaaa
     2821 actttatgca ggggacagag ggcattatcc gcaaggtaga aaacgacctg ttctatgcgg
     2881 taatggaatc ccgctatctg cacgagatca tgaacaaccg gttccatgtg ctggacgagg
     2941 cacggcagat tcgtgtcaac gaccgcatgc acatcacctt ttccattggc gtgggtgacg
     3001 gcggttctac attggcggag agcgagaagt ttgcccgcca gtcgctggat atggcactgg
     3061 gacggggcgg cgatcaggcg gcagtgaaaa cggaaaacgg gttctgcttt ttcggcggtg
     3121 cctccaaggg catcgagaag aagtccaaaa ccaagattcg ctccattgcc cttgccatgc
     3181 aggaactgat cgaaaattcc gatcaggtgt tccttatggg acaccgcttc ggcgatctgg
     3241 attccgtagg ttctgcctgc ggtctggcgg gagccgtgcg gctgatgcag aagccggcgt
     3301 atgtggtggt gaaccagcaa agctgtcttg ccacccagct gatcgaccgc atgcagcagt
     3361 gtccggacgg tccgaagttt atggaaccgg tggacgcact ggcacaggtg acggacaaca
     3421 gcctgctgat cgtcgtggat acccacaaca aggacattct ggaaagcgtg gatctgtacc
     3481 atgcggcacg gtatgtcatt gtcatcgacc accaccggaa aaatgtgaat ttcattgaaa
     3541 atgcggtgat tttccaccac gaaccctatg catcgtccgc ttcggaaatg gtgacggaga
     3601 tcatacagta tttccggctg gataccgagg tttcctcggc atatgccgat gccctgctgg
     3661 cgggaatcat gctggatacc aagaattttg tcatgcgtac cggtgtgcga acctttgaag
     3721 cagcggcgta tctgcggaag atcggggcgg atacggtaca ggtcaagtct ctgttctcca
     3781 atacgatttc catgtactcc atgcgtgccc agattgtggc acatgcggaa ttgtaccgga
     3841 acaacgccat ttctgccgtg aagctacagg acaaaaatgc ccgtgtgctg gcggcacagg
     3901 cggcagatga actgttgagc attcagggcg tggaagcttc ctttgtgctg tatctggaag
     3961 aaaccggcgt gggaatctcc gcacgctcta tgggtaatgt caatgtacag gtcatcatgg
     4021 agcagctggg cggcggcggt caccagacaa tggcggcaac gcagctgaag ggctgtacca
     4081 ttcaggaggc aaaggaacag ctgctgctga ttctggaagc acaggaaaat gaaacataag
     4141 gaaacactgc ataaagcccg cctgacggct ttgcacgcaa tctgcgtgca gattttccgt
     4201 catattgccc agaaatccct tgccatacat acagtatgcc tgcggaattt ctgtgcaatc
     4261 tgccgaaaaa tctgacaacg catcttacgc acaagcttta ttccgtgttt ccataatcgg
     4321 ataagcattt ccttaaagta aagaaaagga gagagaacca tgaaagtaat ttttatgcag
     4381 gacgtaaaag gctctggaaa aaagggcgaa gtgaaaaacg tttctgacgg ctatgcccgc
     4441 aacatgctgc tgaaaaaggg gctggcagta gaagcaacac cgcagaacct gtcggaactt
     4501 tccggaaagc agtcctccgc agcccacaag atcgacgtgg aaaagcagaa tgcacgggca
     4561 attgctgaca agctgaaagg acagaccgtt cacgccggag caaaggctgg caacggcggc
     4621 agactgttcg gggcagttac agcggcacag atcgcggagt gcattgaaaa acagttcggc
     4681 tgcaaaatcg acaagaagaa gatcggtctg aaatcggaca tcaaaaattt cggcagctat
     4741 gaggcagata ttcgcctgta caccggcatt tctgtcaagg tgactgtaga agttgtcgag
     4801 gaataaaggc aacaccgcac aaagagcgtc tggcgacttt gcacgccatc tgcttgcaa
//
LOCUS       DAWBGR010000178         4832 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4214_length_4832_cov_4.136487, whole genome shotgun sequence.
ACCESSION   DAWBGR010000178 DAWBGR010000000
VERSION     DAWBGR010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4214_length_4832_cov_4.136487"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1046 a         1240 c         1324 g         1222 t
ORIGIN      
        1 cgaaaaattt gacgacgcat ctgtcgcaca atctttgtgc ggtattgccg taagagtgta
       61 tccgttactg cactaccgat tcccagcttg tgggcaaacc ggcacttttc tgtgcaatat
      121 acagcaatgc atagtaaata tccgtggaat ccagtgtatc gttttcgtca atatctgccg
      181 ccagaaaagc cgcaccctcc agcagatctt cccgaatgcc ggcggaactc ttggcacagg
      241 agtacagcat ctcgaacaga tcctgcgtgt ccacactgcc gtcagcgttg acatcgccct
      301 tttcgcaggt gagcagttct gcctgcatgg tcagcgtgta ctcctgatcg gaaaaagcag
      361 agtagctttt attttccact ttcacataga acgtgccgcc gcgaaccaga gccgtgtcac
      421 tggtggtctt ggtttgttcc ggtgcagcct gcatggtgag caccggagtt tcccgcaggg
      481 aaccggcttt ttcgtcgtac tgatagcagg tcacagtcca cgcattgccg cttttgctga
      541 ggttgtccgg cagggcatgc tccagcgtca gggaaaactg tgcggcactg tctgatgtga
      601 ccttgtagaa atcggtgtcg ttgacggaaa ccagttttcc ggtgacaggt gcattcaccg
      661 gcagagtgtc ggcattggcg gtggtatcgt tgggttcgct ctcgtaggtg tcaccggtgg
      721 gggtgaaatc cacagtcagc tggtattgtt ccgcatattt ccagttgttt gtcaaggtgt
      781 tgtctgtctt cacctgcacg taaaatattc ccgcgttgaa tccctgttcc ggaatcgtta
      841 ccgaagtgct gctgtttcgc agggcagcct tgcccaccgg ttcctgtgct tcattgtata
      901 gaattatggt gcagttcgca gtgctctggt caggattttc cagtgtgaca gaaatttttc
      961 ccgcctcctc tgtggaaaac gtatagcagt cggcatcatt ggaatcgctg aaaacgcctt
     1021 tgcagggggt cgccagtgca atggggttgg cggtgttaaa attgccgttg ggttccaact
     1081 catagctgct gtcagcggtc cactggcttt tcggagtgaa gtttgccgtc agggtgtagg
     1141 cactctttgg cgggtaggtg tcatactccg gcttcacaga gaagtaatag acacctgctt
     1201 tccagccgtc agccggaaac tgtatatttt ctgcaacata gtcatcctga aaaacggtcg
     1261 tgccgtcggc ggtgtaaaag gcaacgcgat aggattcccc gaagcctgaa acccagtccg
     1321 cagtcatgcc catttggagt gtgccgtcct ccggcagggt gatcttgtag tagtccacat
     1381 caccctcggc ggagatctct ccggaaactg ctgtattcag gggcagttct tccgcggcgg
     1441 cgagggtatc gttttctccg gcagtgtctg cggtttcggc tgtcgcggaa aaggaagccg
     1501 tcaggcagct gatgcagagc atgccggcga aaatggcggc aaatcgtttt tgcttgctgg
     1561 ttttcataaa aataatcctt tctgtggcag acattcagtg tttcccttag tataccacag
     1621 aaaggttaat tcggcatgga gccgtgggtt aaatgttggt gaaaagtgaa tccggctcct
     1681 gcggaagctg cggctgaaat ccgttttccg tcagctgtac aatggcatca tagaggatca
     1741 catgccgcgg gtcgcactgc ttggacaggt gatagtgtcc ggagaaccaa aggcggaaat
     1801 tcagcttttc gtccagctcc tgaaagaaat cggtcagccg gttctgggga taattccact
     1861 ctttgaacag cttttgctgc atttttgtgg aaaggcaatg gctgattacc acgtcaacat
     1921 tccagttcac atgctccagt gcctctctgg catgctgcaa ctccgtttcg gcggggagtt
     1981 cttccgccca ccacgacacg cctgccctgc ggtattcttt gtcacggctt tctgcaccgc
     2041 ccatggtgaa gatgcggcgg ttttccagct gaaatacctg tccgcggcag agatgaaaaa
     2101 tgtgaggcgt gatcttttgg attttgccgc cgtgccattc cgtggcggga tatgtatgca
     2161 gccggtcaaa attttcgtgg ttgccgtcca cccatagggt tgtccacggt ttttcttcca
     2221 gccagttcag ccagcggcgt tcttcggcac agtcctgcca aaccagcccg aaatcgccgc
     2281 agatgatgac aaaatcttcg cgggacagct gcggctgcat gggaaaattg cttgcggaaa
     2341 gcttttgaat gtcacggctg ccgtgtatat ctccagttac aaaaatcaaa gaaatcccgt
     2401 cctttcaggg caatgccact gccttagaat aaaactacgc ccaaagctgc cagcaccagc
     2461 agtcccacgc cgacacagag cagtacaacg cctgccacgc cggcggtgga aaccacgtgt
     2521 tctacgtagg cacgcttggg atttttcggg tcatagacta ccggcagtgt gttgcccagt
     2581 cgccacagtt ctccggcagc agcttccaga ctgtgaaccg ttccggtctg ctgtatgtgg
     2641 aaaacgtcgt tccggacagc aatgcgggtt tctccggcag tgccgtctga aatggggaca
     2701 acttttttgg aaacagctat acctttgtaa tgccggtagg cagtgtaagt ttcacctgcc
     2761 gcctgaaatt ccaccaccgg tgcgatcgat gtaccacagc cgctgcccct gcgttgcagg
     2821 cggtagcgaa ctacggtgcc ggtggtgcgg gcagaacatt tccgccggag catgccggtg
     2881 tgctggaaaa atgggatcga cagcagggca gtcaggatcc ctgcaccgcc aatggcaagg
     2941 caggcaatga ggttgggtgt gagtgtcatg gtttctctcc tttttagtag agaatgcttt
     3001 ttctgatcag ctgtgttaca acaggactgc gttcagataa gattccagtg ataattttga
     3061 aataggtcaa aagcatggaa gcttcctctt catgtaatgc cagcgacaga tgattacaga
     3121 aaagggttag ataaagtctg atattatatc agatatgaaa aatatacttg agccaatcca
     3181 aaaaatactg gcatgcttct tcttctgttt caaacgaatt gaatgaagtg cgtttcccgc
     3241 gttctgaata gtaagtatac cattttccat tagattcttc tatgcaatat gtttcatttg
     3301 gaagaccgcc atttaggtca tatgcacgtt tgtcaatgct taattcattt aactttcttt
     3361 ctaacgcttt ttttgtcatc tttattccgc cttcattccc cacagattcg cagaatcgcc
     3421 gctgccatca tgcaggtgtt ctcctccagc tgctccagcg gcaaaaattc atcgtccccg
     3481 tgcatgtgat agtcccagtc ggggtattcc atgccgaatg ccacgccgcc ggcaatgtca
     3541 tgcacatagg tgccgccgcc aatggcaata cattcgcctt tggtgccggt ctgctcctca
     3601 tagactgcca gcaagtcctg tacaaaaggc gtgtccttgg gggtgtgatg cgggtcgctc
     3661 tgaatgcgtg cttcgcaccg gaatcccttt gcggcaaagg cagcctgtac ctgttccagc
     3721 actgcctctt tggtggtgca gaccggaaag cgaatatcaa cacagccggt gagcatgccg
     3781 ttttctacgt ggagcatgct gcaaatacaa gtgagggcac cggattctgt gtcagcacag
     3841 gcaatgccca gaccgctgcc gtcggtgcag ccgtggggga acagctgtgc cagagccttg
     3901 cagtcggcaa aggcggcatc gctgcctaga taggtcagca gtccggtaat ggcgttgtcg
     3961 ccgctttccg gcgtggaagc gtgggctgca acgcccttgt aggcgagttt gtttccctgg
     4021 caggtaattt gactgccctg ttttgctgca ccgcagaacg ccgcagaaag gctcacagag
     4081 gcatctgccg gaacagcatt gggagcagtg ccggcggaga agcggacaat gggatctgcg
     4141 gtctgcttgg tgaactccag ccggagcata cccttttcca gatgaatgat cggatagctg
     4201 ccgtccggcg tgaatacctg cggcggcatg gcagcatgtg ccagataata ggcgaggtcg
     4261 gtagagccgt tttcctcatt gcagcccagc aggaaccgga cgtttttccg caggggaatg
     4321 ccggcggctt tgatagcaca gagagcgtac agcactgcca cagccggtcc cttgtcgtca
     4381 atggcacctc tgccgtaaat gcggtcgttt cggatctcgg cagtgaacgg gtcggaagtc
     4441 cagttcgcct gtaccaccgg aaccacgtcc aggtggcaca gaattcccag atgcggcggc
     4501 agcgtgtcgt cccagtcagc ggctcccatg tggtagtcat agttggtgca ggtcatgccc
     4561 agtgcggaca gctgttccag cataaagtcc agagcctcgg cagcgggctt gccataggga
     4621 aagccatcct gcggctgcgg tgtggcaacg ctgggaaagc cgatcagttt ggaaagcgta
     4681 tctacggctt cctgccggtg agtgtgaatg taagttttaa ctgcttcagg cagcataaaa
     4741 gttccttctt ccttttcaat atatcgaatg gtaatatagg aaacactgaa taaagcttgt
     4801 gcgtaagatg cgtagtcaga tttttcggca ga
//
LOCUS       DAWBGR010000179         4782 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4274_length_4782_cov_3.628305, whole genome shotgun sequence.
ACCESSION   DAWBGR010000179 DAWBGR010000000
VERSION     DAWBGR010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4274_length_4782_cov_3.628305"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1135..1144
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1385 a          944 c         1110 g         1333 t
ORIGIN      
        1 gccgaaaaat ctgacaacgc accttacgca caagctttgt gcggtgttgc cttagacttt
       61 cttattttgt tgtgtaatca ccgttggcat agaaaattct tgtgcataag atacacatgc
      121 ctttgttaaa ggagtacttg ccttatgaaa gaacgttttg aaaaacaatt gcagagcgat
      181 ttcccgttta tgcagcaaaa tcgggacaaa aaagaaagaa atatatatcg caggtggggg
      241 tgcgaatgca gttcaggctg gttcgaactg atctacgaat tatgtcaaag aatcacgaat
      301 agatatgctg aagaaggtat tccggcaaat gagattgact tggaaattct tcagataaaa
      361 gagaagttcg gtacactcag gttttattac aaatttaaag gcgtatctgg cgaactacag
      421 gctattgatt tcctcggtgt tgcttctttg agatttgaac ctcagaattg tgaggataac
      481 gaagcaaaga agaaactcag gcatgatata tcagaactcg ttcgaggata tgaaaaaagg
      541 tctgcacata tctgtgaata ctgcggtgag agaggtatgt tcagaatgga tatgccatgg
      601 aaaaaaacgc tttgtgatga ttgttataac gcatacttga gaaaatacga agagaaaaca
      661 aaatcgtaaa caacagcatt gctttatgag catctatcta agaacccgtc ttcacaagcg
      721 tatacacgtg tcttttcggc aaattttgct gcatactgcg tgaaaaacac ttgccgggcg
      781 acagcccgcc tgcgtatttt ttccttgtct gcaacaaaat tatgctcgaa aatccacata
      841 tatttcttgt gaagacgggt tctaaaaaat ccccttacca gtattcgctg ataaggggat
      901 tttgcataga tgtggtttta ttttgtggtt tctctgcgga tcagaatcgg tgtaagcacc
      961 ttgtgaatcg gtgcagcaag cggcttgggt ctgcgttttt ttcgttcgct gatttgcagt
     1021 gaaagcagtt ctactgcctc ctgtgcgatt ttgtccattt gttggccaac tgtggtaatg
     1081 ggaaaaatcg cctctctgga aacgggagag ttgtcgaaac caatgatttt atacnnnnnn
     1141 nnnnatttta taccgttccg gcaatgtgcc gtattttcgt ataataagat tgagcagaat
     1201 gttggcgtgg gtgtcattgg gcataaagat tcccactttt ttctctgcat atgtttcttc
     1261 gatctcttgt aaaagctttg cgatctctgc attgttttct tcaaagttgg caccaaaatc
     1321 ccgaatcatg atgcgatgtt ccaacccgtt tgtttcgcaa aaatcctgaa atccctgaat
     1381 gcgtttgtaa gctggaatgg tatttggaaa atctgcattt gcatggagca gaatgtcgca
     1441 gccgcttttg gcgaggacgg ttgcagcctg cacgccgccc atgtagttgt ctgtattgac
     1501 actgcacaca tattcgtctt cccgctcaat ggtaacgata ggaacgtgat attcttccag
     1561 ttctttggaa gagagcgtgt gacttaaaat aatcagcccc tctgtcttat aggcgagaag
     1621 ttcctgaata tatcgccgtt ccacatcggc gtcttcgttc cctgcaaata cgatgaattt
     1681 atatccgtat tcctcatagg tcgagatgat gtgatccagc atatccgaat aataatgcat
     1741 atataagttg ggaatgatca cgccaaccat ttctgttttg ccgtttgcca tgactcttgc
     1801 cagcttattt tccttatagt ccagcacctc taaggcattt gcgatctttt cctgattttc
     1861 cacagtgatg gagtcgggat tgttaaaata tcgggaaatc gttgttttgg aaaaaccggt
     1921 atatgctgca atatctgcaa aggttacatt ttttttcgcc attttttatg cctcctgttt
     1981 ctttcttcat tatagcacat aatcgtttgt gaatcaataa aagaattagt aaagtaacca
     2041 gtttcgtaga actgcacaaa acgttttcat attttttaga aattgcgaca aaaatcagaa
     2101 aaaagccttg acaagtaact agtttcatgg tatgctgtaa tcaacaaagt aactagttac
     2161 tttcaggaaa cgctgcataa agcccgtctg aaggctttgc atgcaatctg cttgcagatt
     2221 ttccgtcata ttgcccagaa acccctttcc agacataccc tgtatacaat agagtgaggt
     2281 gcggatatga acttaaaaaa tgaaattatg ctgattactt atgcggacag catgggaaat
     2341 aacttgaaag aattgcatga actgctgaat caacactatc agaatgctgt tggcggtgtg
     2401 catattctgc cttttttccc atcttctgcg gacagagggt ttgccccgat gtgctacgac
     2461 aaggttgacg agaaattcgg cgacttttca gatgtagagg cattgggaaa agagtattat
     2521 ctgatgtttg actttatggt caatcatatt tcagcaagct ctgtgtattt caaagatttt
     2581 ttggagaaga aagatgcttc agcctataag gacatgttta tccggtatag ccagttctgg
     2641 aaaaacggag aacccacaca ggagcagaca gatctgatct ataagcggaa accgagagca
     2701 ccatatgtgg atgtcacttt ttcggacggc agccgtgata aagtatggtg tacattctgt
     2761 gaggaacaga tcgatctgga tattcgcaca gatgtgacaa agaaatttat ccgggataca
     2821 ctgctctcta tgtgtcatca cggggcatct gtcattcgac tggatgcatt tgcctatgct
     2881 gtaaagaaac cggataccaa ttgctttttt attgaaccgg acatctggga actgctgcat
     2941 gagatcgaag atgttgtcaa aaaggaacat gtagagatct tgccggaaat ccacgaacat
     3001 tacaccattc ccatgaaaat ttccgatcag ggcttttggg tttatgactt tgctcttccg
     3061 gtgctgacac tgcatgccct ctataatcat gacggacaat atttgaagca ttggctggaa
     3121 atgtgtccca tgaaacaatt tacgacattg gacacgcatg acggcattgg cattgtggat
     3181 gtaaaagacc tgctgccgga cgaggaaatt gaaaaggtca aagaacagat gtacagccag
     3241 ggggcaaatg tcaagaagat ctacagctcc gaagcttatc ataatctgga catttatcag
     3301 gtaaatacga cgtattattc ggctttggga aacaatgata aggcgtatct gcttgctcgt
     3361 gcaatacagt tttttgcacc gggaatcccg caggtatatt atgtgggaat gcttgccgga
     3421 agcaatgaca ttgctctgat ggaacagacg aagaacggca gagacatcaa tcgccattat
     3481 tactcaaaag aagaagtggc aaaagagcag gaacgtcctg ttgtacagga actgaaaaaa
     3541 ctaatgacac tgcggaatac ccatccggca ttttctctgg agggaacgat tcaggtaaat
     3601 tctgcaaacg atttgctgac aattacaaga acctttggaa atgattccat tacattgcac
     3661 gcaaacttga cgatttatga ttatacgata gaatgaggga aatgcagtga aaaaatatga
     3721 tgtaacagct ttaggagaac tgctgatcga ttttaccgaa aacggaatca gtccgcaggg
     3781 aaacccctta ctggaagcga acccgggcgg tgctccctgt aatgtgcttg ccatgctttc
     3841 caagctggga aagcatacgg cgttcattgg aaaagtcgga gcagatatgt tcggcaggca
     3901 gctttctgaa accataaaag aggttggaat ttgtgcagac ggactggtgg cagatgcagg
     3961 tgttcccacg acgcttgcct ttgtgcatac ctttgccggt ggtgaacggg agttcagctt
     4021 tattcgtgat ccgggtgcag acatgatgct gaccaggcag gaagtgcgta cagatttgat
     4081 tcaggattcc agaatctttc actttggtac actttcctcg acccatgcag gcgttcggga
     4141 agccaccaga tttgccatag ataccgcaat agaaagcgga gcaatgctct cctttgaccc
     4201 gaatctgcgg gaaccgctct ggaacaatct tgaagatgca agaactgaaa tcgaatacgg
     4261 gttctccaaa tgcaatcttt tgaaaatctc agacaatgaa gtggagttta tgttcgggca
     4321 ttcggattat gaccgggcag caagggaact cttggaaaaa tatccgcaga tacagctgat
     4381 ctgcatcacg ctgggcggaa acggaagctg tgcttacacg agaaatgcaa cagcaaaagc
     4441 accggtctat gatgtgaaat ctgtggaaaa aaccggtgca ggagatacat ttttcggttg
     4501 cgtgttgaac tatgtgctgg agcatgaagt gaacaagctc acaaaagagc agcttctgga
     4561 actgctgcgt tttgcaaatg ccggagcatc gctgattacg acgagaaagg gtgcgttaaa
     4621 ggtgatgccg aatcggcagg agatacagga tctgatgaaa tcggaaatta tttcagaagc
     4681 ataaaagcat aaggcaatgc cgcacaaaga gcgtctggcg actttgtacg ccatctgctt
     4741 gcaaatttcc cgtcatattt cacaaaaatg ctcgtgaata ca
//
LOCUS       DAWBGR010000180         4708 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4360_length_4708_cov_5.004083, whole genome shotgun sequence.
ACCESSION   DAWBGR010000180 DAWBGR010000000
VERSION     DAWBGR010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4360_length_4708_cov_5.004083"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1113 a         1202 c         1242 g         1151 t
ORIGIN      
        1 tttgcaagca gatggcgtgc aaagtcgcca gacgctcttt gtgcggtatt gcctctatta
       61 taacggatca aagcgttctt tttcaagggg gattttgtga aaattacttc ttgccgccgt
      121 cctcggggaa cagcttgctt ttcagcttct tcaggctgta gccctccttg accttgaggt
      181 ctgcacgttc cacagccagt gcgtaatccg gcacgtcctt tgtgaccgta gaacctgcgg
      241 cagtataagc acccttaccc agttctaccg gtgcgatcag gttggtgttg cagccgataa
      301 agcagttatc accgatcaca caacgctgct tcttcagacc gtcatagttg acggtgacag
      361 tgccgcagcc gaagttgact ttcttgccca cgtcgctgtc gcctacgtat gtcaggtgag
      421 caatggcagt ctgctcgccc acggtggagt tcttgacttc cacgaaatcg ccgatcttga
      481 caccgttttt caggtgtgaa ttcggacgga tatggacaaa cgggcctgcc tttacagcat
      541 cttcgatctc ggactcgaac gcctgtacat agttcagcgt cacgtggtca ccgatgttgc
      601 agttttcgat aaggcagttc gggccgatgg tacacttggc accgattctg gtcttgcctc
      661 ttaaaatggt acccggcaga atggtggttt ccgcaccgat ctccacatcg ttgccgatga
      721 tgatgccgtc ggtgttgaca aagttgacac cgttttccat gtgcttctcc agaacccgtt
      781 cctgtgccac cttgttcaga tgcagcaggt cttttctggt gttggcaccc aggatcacgt
      841 ccggattctc gctgcgatat gcactggctt tccagccccg ttccagtgcc agtcccacag
      901 tatcggtgag gtaatactcg ccctgtgcgt tggactggga cagattcccc agcagttcca
      961 gcagagcctt ggtcttgaac cagtacgcac cggagttcac ctcgtggatc tcccgctgtg
     1021 catctgtggc atctttttcc tctacgatcg ccgcaatgcc ggtttcgctg cggagaattc
     1081 tgccgtagcc ggtgggctgt tccaaagtgg cagtgatgac tgtcacggca ttttcctgtg
     1141 cctcgtgcag cttcagggca ttggcaatgg tttcgctgtc cataaacggg gcatcaccgc
     1201 agagcaccag tgtgttgccg tcggtgaatt cctgcatgaa tttttctgcc tgcatcactg
     1261 catgaccggt gcccagctgt tccgcctgca aagccgtccg atagcggttg cccagataca
     1321 gttccacgta ctgtgcctta tagccggtca ccacgcagat gcggctcaag cctgcaccct
     1381 cgcatgcacg gatcacccag cccagcatcg gcacgtccag tacctgcagc atcggcttcg
     1441 ggatcgcagc cttcatacgt ttgccgtgtc cgccggcaag aataacaacc tgattcatgt
     1501 tttatgtcct cctgtaatac ccccctccgg aaatttccgg agcagagcga ttttgcatca
     1561 ttgcatggat tgggtgctcc tccggacagc cgtctgaaaa agcccctttt tacattatga
     1621 caaaattttg aaaggattgc aagtcttttt tcaaaaaaaa ttcgtgtatt gtgatttttg
     1681 tataacttgc cacaaaaatg ggcgtaattt tttcttgaaa agtgtatagt attttcatga
     1741 gaagtatgct ataataataa tgagccggtt gagaaaggga aatttgccag atttccccga
     1801 atgcaatcac gcagccggac attcaatcga aatcaaaaat tggaggtatt taccaatggc
     1861 aaacaaatgg gtttatatgt ttagcgaagg cgacatgacc atgcggaatc tcctcggcgg
     1921 taagggtgca aacctggctg agatgaccag catcggtctg ccggtaccgc agggtttcac
     1981 catcacaact gaggcctgca cacagtacta tgaggacggc cgcaagatca acgacgagat
     2041 catggctcag gtcatggacg gcgttaagaa gatggaagag atcaacggca agaagttcgg
     2101 cgatctgaac aatccgctgc tggtttccgt tcgttccggt gcacgtgctt ctatgccggg
     2161 tatgatggac accatcctga acctcggtct gaacgacgac gttgttgctg caatgatcaa
     2221 gggcaatccg gatccggctt tcgagcgttt cgtatatgac tcctacagac gtttcattca
     2281 gatgttctct gacgttgtta tggaagtcgg caagaagtac ttcgaacagc tcatcgacaa
     2341 gatgaaggaa gaaaagggcg taaagttcga cgtagagctg accgctgctg atctgaagac
     2401 tctggctgaa cagttcaagg ctgagtacaa gaaccagctg ggcacagact tcccgtctga
     2461 tccggtagaa cagctgaagc tggctatcga agctgtattc cgcagctggg acaacccgcg
     2521 tgcaaacgta tatcgccgtg acaacgatat tccgtacagc tggggtaccg cagttaacgt
     2581 aatgccgatg gtattcggta acctgaacaa cgagtctggt actggtgtag cattcacccg
     2641 tgacccggct accggtgaaa agaagctgat gggcgaattc ctgatcaacg cacagggcga
     2701 agacgttgtt gccggcgttc gtactccgat gccgatcgct cagatggcac aggaattccc
     2761 ggaagcttac gatgaattca tcaaggtttg tgataccctg gaaaaccact atcacgacat
     2821 gcaggatatg gaattcactg tcgagaacaa gaagctgtac atgctgcagt gccgtaacgg
     2881 taagagaacc gcacaggctg ctctgcagat cgcttgcgac ctcgtagatg aaggacataa
     2941 gacagaagaa gaagcagttc tgatgatcga tccgagaaac ctggacaccc tgctgcaccc
     3001 gcagttcgat gccaaggctc tgaaggctgc tactccgatg ggcaagggtc tgggtgcttc
     3061 cccgggtgct gcctgcggta aggtcgtatt cactgctgac gatgcagaag cttggaacgc
     3121 aaagggcgaa aaggttattc tggttcgtct ggaaacctct ccggaagaca tcaccggcat
     3181 gaaggcatct cagggtatcc tgactgtccg cggcggtatg acttctcacg ctgccgttgt
     3241 tgctcgtggt atgggtacct gctgtgtatc cggctgcggc gacatcaaga tgaacgaaga
     3301 agacaagcag ttcgaactgg ctggaaagac cttccacgag ggcgactaca tttctatcga
     3361 cggttctacc ggtaacatct atgacggcat tatcccgact gttgacgctt ccatcgccgg
     3421 cacattcggc cgcgttatgg gctgggcaga taagtacaga actctgaagg ttcgtaccaa
     3481 cgctgatact ccggctgacg caaagaaggc tcgcgaactg ggtgcagaag gtatcggtct
     3541 gtgccgtacc gagcacatgt tcttcgaagc tgacagaatc gcagcattcc gtgaaatgat
     3601 ctgctctgac actgttgaag aaagaaaggc tgctctggca aagatcgaac cgatgcagca
     3661 gggcgacttc gaggctctgt acgaagctct ggaaggcaat ccggttacca tccgtttcct
     3721 ggatccgccg ctccacgaat tcgttccgac cgaagaagaa gacatcaaga agctggctga
     3781 tgctcagggc aagactgtag atcagatcaa ggctatcatt gcttctctgc acgaattcaa
     3841 cccgatgatg ggtcaccgtg gctgccgtct ggctgttact tatccggaaa tcgctgaaat
     3901 gcagaccaag gcaatcatca aggctgctat caacgtaaag aagaatcatc cggattggac
     3961 cgttaagccg gagatcatga tcccgctggt cggcgatgtg aaggaactga agttcgtaaa
     4021 gaaggtcgtt gtagaaactg ctgacgctga aatcgctgct gctggttctg acctgtctta
     4081 tgaagttggt accatgatcg agatcccgag agctgctctg actgctgacg acatcgcaaa
     4141 ggaagctgac ttcttctgct tcggtacaaa cgacctgact cagatgactt acggcttctc
     4201 tcgtgacgat gctggtaagt tcctcggtgc atactatgat gcaaagatct tcgagagcga
     4261 tccgttcgct aagctggacc agactggtgt tggtaagctg atgaagatgg ctatcgatct
     4321 gggtaagccg gtcaacagca agctgcactg cggtatctgc ggtgagcacg gcggcgaccc
     4381 gacatctgtt gagttctgcc acgaaatcgg tctggactat gtttcctgct ctccgttccg
     4441 tgtgccgatc gcaagactgg ctgctgcaca ggctgcaatc aagaacaagt aagtttgatt
     4501 cgttcgttta tccacgcagt cgcctgactg cctgactccg gacatgtgca ggacctgacg
     4561 gacaagttat ggacaagtca ctgtcgaaaa tgattcccga acaattttga tatatcgaaa
     4621 aaaatccctg tacatcggac gtgatgtaca gggatttttg gcaataccgc acaaagattg
     4681 tgcgacagat gcgtcgtcaa atttttcg
//
LOCUS       DAWBGR010000181         4648 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4432_length_4648_cov_3.124537, whole genome shotgun sequence.
ACCESSION   DAWBGR010000181 DAWBGR010000000
VERSION     DAWBGR010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4432_length_4648_cov_3.124537"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1873..1882
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1137 a         1023 c         1011 g         1467 t
ORIGIN      
        1 aggtgtagct cagtttggta gagtgcttgg tttgggacca agatgccgca ggttcgagtc
       61 ctgtcacctc gaccatatgt aaaacggctt agatgcgaaa tttgacgcat ttaagccgtt
      121 tttttgtgct ctgatatttc cggcggaaga tggatttttc ttaaaaatgc caactttttc
      181 gggtatgtgc caacgaaaag tgccaactga aaaaaacaag ggacttcgaa aagccccttg
      241 cattcttgtg gcttctgtca tttttcaatc gttcttgtcg attacgacct tgttgaagta
      301 atcgtctatg atgccgtctg ccttgttgcg ctcgctgtca aaggtttcct ggtatacgtt
      361 tttgtataca tccggtgtac tccagccgcc catctccatg gcatatttct ctggtacatt
      421 cagcatggca gccgtacttg caaagatatg tctgcaatca tgaaagcgaa tatcctcaat
      481 accgttgtct cggagcagtc gcttgaatcg ctttcggatg gtattgggtt tgaccgaaat
      541 aatgtggtct gtctcttttt ggtgtgggat tgcctgaatt aagttgtaga tatgtatggg
      601 aagatttaca cagcgcacac ttttctctgt tttgcagtgg ttcacttctg cccagccgga
      661 atcaactttt acgatctcac ggcgcacata cagacgcatt gttttggcat cgacatctct
      721 gaattgtagc ccagctactt cgccgatgcg cagtgacaac cacattgcaa gcagaacagg
      781 caattcactg tccgtgccct tgacgatctt gtagatttcg ggcgctgtcg gaagcttacg
      841 ttctcgaacc tcttttttcg gtaatctgat gctgcccagc ttgatatcaa tgtcatttgc
      901 aatcaaaacg cttttcagga atccataagc attcttgatg gatttatgac tgagacggtg
      961 tgcatccaga ttgattgcga tttggatatc ctttgccgtg agttcttcga tattgatgtc
     1021 catgatgtac tgtaacttat tgcggacaag ttctctgtag ttgcagatcg tagtcggttc
     1081 aagcacacgg cttcggctgt caataaattg cagcattgcc tgctttactg tgcatggtgt
     1141 gttcttttct tcgtcaatgt gtttgagcca cttcgtagcc ttgataatga cttcatttgg
     1201 cgtactgcct gtgatcttgc tctttttgcg gtattttcca cttccttcag atacccaggt
     1261 atagaaattg ccgctgtctg tttgcttgat gttgaacttc tttttcattc tgattgctcc
     1321 ttttcaaaaa aagagcagac ggtacctcgc atttaaggta tcgtctgcgt tatgtgttat
     1381 gcggtcatta attcttccag tgtcaactcg ttcacgcaga ggaacgtgtc cactgtcttt
     1441 aatgtaaggt ttttggcatc atgatcgtag ttctggagcg ttctcgtgga acagttcaga
     1501 tacattgcca gttcatcgtc agtcagatca tgaagcagct gccagtagcg aatcttgcac
     1561 cagatggtct tctggagcgg aatgcgcttt ttcgttttca tagccattgt ttgtccctcc
     1621 tcacggcaat aatatatgtt tgttttgggt gatatttaca catcagcaga cagtgctgaa
     1681 gagcttctgt ctgtgatctc aaagcgtctg atctcaggag atacgatttc atgaatgcct
     1741 gcaaataaga ggatatcatt cacagtgtcg atctggatat tggatttcaa ttccttcgga
     1801 taagcgccag tttcattgca ggcggctttc agttcctcct tgagggcctt caaagcatcc
     1861 ggatcacagt tgnnnnnnnn nngacttata tttgccccag gctgatacga tgcgctgctg
     1921 acaagccttt gtgatatcgt aatccaggaa taattcttcc atgccttcgg ttggctttcc
     1981 gtaagtctcc aataccttgc gtacaaaggc atgggaattt ttttgctctt ctgtcttcaa
     2041 ggctcttatg tgattcagtt gggttcgcag ctgctgaaga ccaatgcggt tcaccagagg
     2101 cttttttaag atcgcatcat agcaaacata tgggttaggc tggttcagat actgcagggc
     2161 aaagtccgga ttatctgctg ccttttggaa tgcagacagc tgcatataaa gcttaccctc
     2221 catatttccc agaagactaa ggtcatagtc cgggataagc acctgaattt cacgtggctt
     2281 tttgcggcca actcttgcac ggcctatgat ctgttgtaac tcgaccatat cacaagtttc
     2341 cgctacaata agagacaggt tgtcatcatg aattgaccag ccattttccg ctacctttgt
     2401 tgtcaccagt gcactcgcat cgaaaccttc ctgcaaagca atcttttgga tctcatcctt
     2461 cttgtcatca ctggaataga tacgctgact gtctcccagc agctcttgca tctctgcgcc
     2521 agcttccatg tcgttgatgt aaatcagtgc cttttttcct tgggcgcaga atttctgaat
     2581 gtattcagcc aattccttac gtttggctgg gtagtatact gcaaatttca gataagtcca
     2641 gtccgactgc atgatgtagg tatgtttgat tctggtgcag tctgcatctg gtgttactcg
     2701 tagaaaatgc tgtatattgt cagggtcaaa ctgacagaga ggcttatcgg acaaattctc
     2761 aatgtcccat agaataggaa gaacatcact tggcgttgct gtaatataga ttcgtttcgt
     2821 attgtcgagg ttgccattga gaaaattcac cacatgctgt ggataaagag aaaacgttgc
     2881 atcttccgca aaacagtggc attcgtcaca gatcaaaacc agcttttttc cttggtactt
     2941 atgctttttt cttgcaaatt cctggtatgt catgacttcc acattatcat tgacctttat
     3001 tttttcgatc aaggctcccg gaatattcgg gcatttgatc aaatccttga gcacctgtgt
     3061 tttgcatatg cagcggtttg ccaggacaat gagatgtgaa tccgaggata aggcggctat
     3121 gatcaattcc atcgcttttg tcttgcctga gccagtcggt gagaagacac acaaggattc
     3181 accgtattcc agcttgtcca catctttcgc attgatgatc gaagtcagat attccttaga
     3241 accgccaagc gtagagcctg ttatcaggca gagggacgca agcattggat tcttcacatt
     3301 tgcaatctct gccgcatgag cagagacaac tgccgggtcc attttttctg ggtaaatcat
     3361 aatagacata atttttcctc ctttattgct gcggcgttac gcatgaagct taacgccgta
     3421 aaaaccacgg ttgttctgtc cgccaattcg ttttctggtc ttttccagtt tttctacgtt
     3481 gacacggtaa aattcctggg aaaagcttgt gatatcttga aatacgctgc catatacagc
     3541 gcagaatgcc atatacaagt caaaggtact tgtaaaagca tccggatcgg aaaagtcaca
     3601 gtttgtacag ctgaaaactt ggattgcatt gtaggcgctg ttcagtggta caaacagtga
     3661 cgactgcatt gtatatgcag tacagtttgg gaatttatag ttgttttctc tgagtgccag
     3721 ataactgcga aatgcctctg tagcgatggc atctttctct gccagcagct tttccagaat
     3781 atatggatct tgctgttcct ttagaatgga aaccggataa ggaattgtaa caattcttct
     3841 tgcaaaagcc ggatcgatat tggaatacgc cataccgata ttgttgttgc tggaaattac
     3901 gattttgctg gtacagggaa aggatacaaa ttctttgaac ttgacatttg ctgtcaaatc
     3961 atcatgtcct gaaaaggttt tcagataacc aatatctctt gcgcccaggt ctaattcgcc
     4021 ttcatctgct gagatttcca ttctgatatt acgcagacgt gctttgccaa actctgataa
     4081 gagcgttcta atgggcataa agtctacact tccttttgac agaaggtttt tttgcagatt
     4141 cagctctgta gattttccat tgttgccaga aggtccatat agaaagaaaa gggcttttgc
     4201 atgagcatct gaagagagac agtatcccca atatttccag tgcagatcaa tcaacactgg
     4261 gtcaccaccg ctaatggtat acaggaactg atctgcagct gggtgattca acactggatc
     4321 tgtcacatag tttgcctcta cgcaaattgt ggagaaataa tcatctggca ggctaacgat
     4381 ttgaccagtc agattggaaa cgcagcagtt tctgaaaaca gtatactcct ctccgtttgg
     4441 gattccgtta aattttagac ttcgaagcat cacctccttc ataacatcag cgcagttgga
     4501 aaacgtcaat gttaccccat cctgagccgc tagttgatat atccagttgt aaaattcgtt
     4561 tttgtcaacc tttacatcct ggcatccatt gcgcacataa agatcgcctt catagatcat
     4621 aaagtgatgc gtactgcata tataatcc
//
LOCUS       DAWBGR010000182         4625 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4454_length_4625_cov_4.698906, whole genome shotgun sequence.
ACCESSION   DAWBGR010000182 DAWBGR010000000
VERSION     DAWBGR010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4454_length_4625_cov_4.698906"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          985 a         1319 c         1206 g         1115 t
ORIGIN      
        1 cacttgtact gtaagattcc aatatcacac gaagattaca cagagaatct tccactgatg
       61 taaaatcttt tgtttgatgt tgagagttac gtaggaacct ctccaccgcc taacggcggt
      121 ccccctcccc tttcagggga ggctttttcc cttgtgaatt ttcatcaaca aaaatctcag
      181 ggtcagcggc ggcttgctat ccttgggcaa gtctgccgtc gccatttccg ctgggcgacg
      241 ggctaactcg ccgcaccccg gcggggcata caggcggctg ctgccgccgt gacactaccc
      301 ttttcacatt tgaaattggg gcgtaaggtc agcggtgact caccgcaaaa aagcgtgaag
      361 cttccctgct ccacgctttt tcactgcaat ttgccttata tcctcaatta tcgctcagat
      421 catatggttc ttcctgattg gaaaacgccg catcgagaat ctttccgtaa aatcccgccg
      481 gattcagcat gatctgttcg cccagatact tggcttgggt atagtccggt gcatagagtt
      541 tcatgtccag cagatcgtcc tcaatgtcca gacagcgaac atgcacatag tagccttttt
      601 tcagcgggat atactcgatc ttgacttcgc tctcccgctt gatgcgtgcg aaatagtgga
      661 acgccgccga aaccagcttg tcccgatacg cctttcccac atagtttcgc agttcctttg
      721 ccgtccgaat cccgttttct gtcagtacat agtaatcgac agctttctca ttctgacgca
      781 ctgccacaga attgtgctcc gtcagataac tgatcgaatc ctgataggca aaatagttga
      841 taatgttgct tcctaccgca atatcgtaca aatggctgac ctgcaccggt ttatcgatct
      901 tatacagcaa ataacaaatc agaatatttg ccgtatgcgg atccatgatc ggcggcgttg
      961 ccattttcga cattttcatc atattttctt cctgcacagc ctgtcacctc gcatctcaca
     1021 agtgcaatct acggtgtttc ctcaaaaatg cggataatcc atcatatggg aaagcagagc
     1081 gatgttcacc gccgcttcca cacagggcac tgcacggggc acaatgcacg ggtcgtgccg
     1141 tccctgaatc tggagcatct cgtttgtctt tgcccgaaaa tccacggttt cctgcggctt
     1201 ggcgatcgaa gcggtgggct tgatcgccac attcagcgtg atcggcatgc ccgaagaaat
     1261 gccgcccaga ataccgccgt ggttattggt ctttgtcatc acgtggccct tttcgtcaat
     1321 atagaacggg tcgttgttct ggctgcccac catttcggca acctgaaaac cggcaccgaa
     1381 ttccagcccc tttaccgcgg gaatgccgaa gatcagctgg gcgatggtgt tttccagtcc
     1441 ctcaaaaatg ggcgagccta caccggcagg catattgatc gtgacacact caatgatgcc
     1501 gcccagagat tcgccgccgt ggctggcgtt gcgaatgtct tctttcatct tttcaccctg
     1561 tgcatcgttc cgcaccggaa aatccttttc ccgtacttcc agaatgtcct ccttggtcac
     1621 cttggttcgg ctgaacgcat catcatggat tccgtggacg ctggcaatat gggcaccggt
     1681 gtaaatgccg cggcgttcca gaatctgtcc gcagacggct ccggcgaaac acagcggtgc
     1741 agtcagtctg ccggagaaat gtccgccgcc gcgaacgtca ttgaacccgc ggtaccgcat
     1801 ggcaccggta tagtccgcat gaccgggacg gggcagtctt gccagattgc tgtagtcctt
     1861 ggaacgggta tccgtgttct ggataaacgc acacagcggc acgccggtgg tgcggtcatt
     1921 cagcagaccg gacatgatct gaggcagatc tttttccccg cggggtgtgg tggtgccgtc
     1981 ctttttcggg gcacggcgtg ccataaactg ccgcagcttt tccagatcaa tgtattcccc
     2041 cggcggcaga ttgtcgatca cgacaccgat cgcaggtccg tgggactccc caaaaataga
     2101 aagggaaata ttacgactcc agaacgatga cattggcgat tcctcctaac tgacgataat
     2161 cctcatagaa ccgaggatat gatttttcca cgcattctcc atggagaatg gtcacttctc
     2221 cggcacagcg tgtggctgcc accgcagcac acatggcgat acgatgatcg ccgcagctgt
     2281 ccacggtgcc gccgtgcaga tgttccaccg gttcgatctc cagaccgtcc ggtgtggcgg
     2341 tcaccttgcc gcccagccgg ttcagcaggt cggcagtcga tgccagccgg tcgctctcct
     2401 taatggcaag gcggtgtgct ccggtgatgc tggacggtgc cgtaccaaat gtggcaagca
     2461 ctgccagaat cggcacaagg tcaggaatgt ctgccgcatc tacggtaaat cctgccggca
     2521 gcccctgttc tctattataa cacatctcgc ggacaatttc cacaattttt ttatcgccct
     2581 gcacactttc cggattcaga ttggtgaggg tgatgtcgct gcccagtgca ttcgccacgc
     2641 agaagaacgc cgcctgtgaa taatccccct ccactgtgag gtcacaggca tgatactgct
     2701 gtccgccggc aacagcgtaa ccctgttcgg tttcctgcac ggcaatgccg aagtgccgca
     2761 ggcaggcaat ggtcagatcg acatagggct tggattccag cttggacaac atgcgaattt
     2821 cggaatcccc gtccagcagc ggcagggcaa acagcagacc ggtcacatac tgggaagaaa
     2881 cgtcgccctc cagttcgaac acgccggatc gcagcttgcc gtcaatggta aagggcatgg
     2941 tgttgtggta gtcgaacgtg atgccctttt tggtcagttc ccgcaggtac gcggtaatgg
     3001 gacgctgagg cagtctgccc tgtccgaaaa aggtggtgtg tatacccagt gctgctgcca
     3061 cgggaatcag gaaccgcagc gtagagccgc tttcgcagca atccgccaca gccgcctgcg
     3121 gtcgggcatt gcccacgccg gttacggtaa tggtgctgcc ctcctgccgg aacgatgccc
     3181 cgaacgcaga gagcacgtcc atagtggcgg tgatgtcacg ggacatatcc acgccggaaa
     3241 tgactgaagt gccctctgcc agtgcggcac agatcagcag ccggtgtgcc atgctcttgg
     3301 aagcagggat ccgcacggta ccatgcaggc gggacggcgt aatcagaatg ttcatgcgtc
     3361 cacgcctccc atccatgcgg caaattctgt gaaaggcatg gagcgaatct ccgccctgcc
     3421 gatggtttcg cagacgataa accgcagcga tgcactggca cgtttcttgt cattgccgca
     3481 caggggcacc agttccgcaa cgggagcgtc tacttctgtg ggcaggtcgt atgcctttac
     3541 gcagtcgcac agccgcacat acagttccgg cgtacaggtc gctttggtca tcatgcacat
     3601 gccggcggca actgcactgc cgtgggtata ggtttcgtag tggtagtagc tttccaccgc
     3661 atgtcccagc gtgtgcccga agttcaggat catacgctcg ccggtgtcga attcgtcgtg
     3721 ttccaccacg tcccgcttga tgtcgatgca ggtgggaacg atctcgtcca tgacttcgct
     3781 gacggtttcc agcgtgtgct gttccagcag tgtgaacagt ttttcgtccc gaatcatgcc
     3841 gtacttgatg acttcgccca tgccgtctgc cagaaacagc ctcggcaggg tgtgcagggt
     3901 gtccagatcg cagagcactg ccaccggctg tttaaaggct cccaccagat ttttgccgcc
     3961 ggtcaggtcg atcgcagtct ttccgcctac ggacgaatcc acctgtgcca gcaggctggt
     4021 ggggatctgg agatagtcca gaccgcggag ataggtcgcc gccgcgaatc cggtaatatc
     4081 tcctaccacg ccgccgccca gtgcaaccag acagtcggtg cgggtgatat tattttcaca
     4141 gaggaagtcg tagatctggt tcagcgtttt gctgcacttg gaagcttctc cgtgtgggaa
     4201 cacgaacttg atcgtccgga agccattttc ttccagcgac ttggtcacaa tgtccccgta
     4261 atagcagtct acatggtcat cggtaatgac agcggcagtc tgtgccttgg tctgctgttt
     4321 cagatatgtg ccacactgtc ccagcagtcc tctcccgatc caaatgggat acgtctggct
     4381 ggcgtgcacg gtaattgttt tcatggtatt ttcctcccgt tggagccgaa agcctgttct
     4441 gcatctgacg tgttcagacg cgtggcaggc tctgaaagcg tacacatctt cgaatagatt
     4501 ctcatatttt attatactaa aaatcctttt tcttttcaac aacaaattgt aaaccatgca
     4561 aaaatgcggt gagtcaccgc tgaccttacg ccccaatttc aagtgtgaaa agggtagtgt
     4621 tacgg
//
LOCUS       DAWBGR010000183         4541 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4545_length_4541_cov_3.753232, whole genome shotgun sequence.
ACCESSION   DAWBGR010000183 DAWBGR010000000
VERSION     DAWBGR010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4545_length_4541_cov_3.753232"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3959..3968
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1029 a         1083 c         1001 g         1418 t
ORIGIN      
        1 ctgtagttgc ctcggttgtt gcggttgttt cgtttgtggt agttgtttcc tcagttgtcg
       61 cggttgtttc atttgtggta atagtgcttt cctcggtggt ggcagatgtt tctcttgtgg
      121 taacagtgct tgcctcagtt gttacagtcg tttcgtttgt gatagtagtt tcttcagttg
      181 ttacagtcgt ttcgtttgta gtagtttctt cggttgttgc ggttgtttcg tttgtggtag
      241 tagtttcctc agttgttgcg gttgtttcgt ttgtggtagc agttgcctta gttgtcgcgg
      301 ttgttccgct ggtaacagtc gttgccttgg tcgcagaagt cgttccgctg gtggcagtcg
      361 ttgccttggt cgcagaagtc gttccgctgg tggcagttgt tgccttggtc gcagaagtcg
      421 ttccgctggt ggcagttgtt gccttggtag tggcagttgt ttcctttgtt gtggaagtcg
      481 tttcgtttgt agtggtagtg gtctgtgcat ttaccacaac cgtgccggtc acaacattat
      541 agttctcgct gtcctccggg gtgaactccc atgccagggt atttttaccg gcagccagtg
      601 ctttgagttc actgacaatc tttgccgcac ccttggtgct gtccttgctg atgctcagcc
      661 ccagcttttc cacaaggtcg ccctcggtgt gggtgtcgcc gtccacagta acttctaccg
      721 tcgggtcagc ctttgccacc aggaaatgca aggtcaggtt tgcggtgttg ccgtttttcg
      781 cggtaaatgt aaaggtcact tccctgccgt ccgtgtacgc ctttgcaggc ttgccggaaa
      841 gcttactgcc gtccaactgc atgccgtcca gcatggtaga accggttgca accttgacgg
      901 aaacttctcc cactgcgtta gcatttttca cgtaatcctg caagttaatt tcgtccatgt
      961 cctgaccgta aacgcacttg atctgcgtgg tcgctgtggc aacctgaacc ggaacaggtt
     1021 tcacaatttc cgacagttct acctctttgg cagcttctgc gtcggtttcg tcagacagga
     1081 aatacttttt gcagttctca caggtccagt attccacgtt tccggcttcg gtgtaagtcg
     1141 catccttggc atcggttttc acgagagaat gacgatatgt taccttttca gatgcttctc
     1201 cgaaatcgtc ttttgccagc gtagaatcgc aggaaaccgt aatttcaagc gatttatcgc
     1261 agttatcaaa tatatcaatt ccgatgcttg tgacactact tggaatcgtg atgctggtca
     1321 agttggtgca tgcataaaac gcaccacttc caatgcttgt gacactttct ggaatcgtga
     1381 tgcttgtcaa gctgctgcaa ccggaaaacg cagattctcc gatgcttgtg acactacttg
     1441 gaatcgtgat acttgtcaag ccagtgcagt tggaaaacgc agattctccg atgcttgtga
     1501 cactacttgg aatcgtgata cttgtcaagc cagtgcagtt ggaaaacgca cattctccaa
     1561 tttctgtgac atttttggga atcgttactt tcccctgaca agtttgtcca tcgattaaaa
     1621 ttccatttac aatcacaagc ggattttcat ctcgtttgtt tcttaaccaa ggcgtgttag
     1681 aaaacgcatt cctttcgatg cgtgtgacac tatttggaat ggtaatgcta gtcaaatttt
     1741 tacaaccaga aaacatactc ctttcgatgc gtgtgacgct ctccgggatt tcaatgttgg
     1801 ttaagctggt gcaattctga aacgcccttt ctccgattct tgtgacgctc tccggaattt
     1861 tgatgctggt taagctgtca caaacgaaaa atgcacaatt tttgatattt gtgacactat
     1921 ccggaatcgt aatatttgtt aagtttttac aagtaaatgc aaaatcgcca atgcttgtaa
     1981 caccatcttc aatcacaact ttttttattt tactattgtt gaatacaggg ctattataag
     2041 ggcgacgacc ttcaaggtta tcatcgtaag atttcattgg ccctgtgccg ctgatggtca
     2101 ttgtgccatc ggtgttcagt gtccaagtca aaccatcatc ttcgttcagg cggattatga
     2161 ctttgcttgt atctacgccg agatcattgt atgtcagcgt agagttgcct tccagaagca
     2221 cctcggtcaa agcagtacag ccattaaacg cattcgttcc gatgcttgtg acactacttg
     2281 gaatttcaat gtttgctaaa ttgctacagt tctcaaacgc agaatatccg atgcttgtga
     2341 caccttctgg aattatgatg ctggtcaagt tggtgcattt ctgaaacgca gaaactccga
     2401 ttcttgtgac atcgttcgga atcgttactt ctcccgaaca agttgtgccg tcgatcaaga
     2461 ttccatttac aatcacaagc gggtttcctt gctgttcgtt tgttaaccaa gctgtgccag
     2521 aaaacgcacc ttctccaatg cttgtgacac tacttggaat cgtgatgctt gccaagctgc
     2581 tgcaaccgga aaacgcagaa cttccaatgc ttgtgacact atttggaatg acaatgctag
     2641 tcaagctact acaaccagaa aacatatccc attcgattct tgtgacacta tttggaatga
     2701 taatgctagt caagctgcta caattggaaa acacaaaaaa tccgatgctt ctgacactat
     2761 ccggaatttc gatgctgatt aagccagtgc aaccggaaaa tgcattattt tcgatgcttg
     2821 tgacaccatt tggaatttca atgattgcta aattgctaca gttctcaaat gcagaacttc
     2881 tgatacttgt gacaccatct ggaatggtga tacttgtcaa gctggtgcaa ttgtaaaacg
     2941 cagaacttcc gatgcttgtg acaccactcg gaatttcgat gcttgctaaa ttgctgcaat
     3001 cggaaaacgc agaatctccg atgcttatga cactattgga aatcgtgatg ctctctaaat
     3061 tgttacattc acaaaacgca taattaccga tacttgtaac gccatcttca atcacaactt
     3121 tttttatgtt gctattgctg catgcaggac tatccgtcat ctcccctttg ccgctgacgg
     3181 tcattgtgcc ctcagcgtcc agtgtccaga tcaggttgtc ttctgtccat gtctgcgttc
     3241 ccgattctgt ccctgcacct gcatcctccg cactcgccgt gatcccccac gagatatttt
     3301 gcagggtatc ggtcgggaag tacagcagcg ttcccaccgc catgactgct gccgacgaaa
     3361 gggcagccag ccgtttccat tgttttgtca tattttcctc ctttttgttt gaatgtatta
     3421 tatattctga atgatattat tatagcacgg caaggcagta tttgtcaata gaaaaagtgt
     3481 atttttccat tcttttttga aaaatagtat cattctatct tcttaatcaa aaagatgcag
     3541 tattgcctta tatcactgcc cgttcaatcc ggcaagtatg ccgcttctcc ttatcataat
     3601 tcacgccaac caaaagaacc tcgccggtat aattcttcag gcaatccaga taatccttcc
     3661 gcttgatctg ttccactgcc tcttctgcgg aatgccccca cttcagctca acgatcagag
     3721 caggcaaatc gcagtttcga ttcggctcaa acacaatatc tgcaaagcct tttccagccg
     3781 gcatttctcg gtgcagggtg tagttcttct gagcagagta gtatgcaagg gagatcacgc
     3841 aggacagagc gttttcatcg ttgtatttca agatcgatgc attcgcctga tgtgcctgtt
     3901 cgatcagttc ggcaactgtt tccgcatcgc cggaaagggt agcttgcagc agccgctcnn
     3961 nnnnnnnnct gtacaactgg ctcccagccg ccgtcttcaa tggaattgat aaactcctgc
     4021 cgcacttctc cgttcggaat ccacaccaca cgattctgtt catcataggt caagtatccc
     4081 agatgcacca gcagcgtaag aacatcatcc gcactgtgga atgtggtcat gtcattctgg
     4141 aacttatccg gattcacagg cactttttcg cctgcgatca tctgtacgat cttgtcacgc
     4201 agcccgtcct gattcaggtc aaggtacact ttcagtgctt cataggtttc tgtctttgtc
     4261 cagtagttgc tgaattttcc acggagcata gcttctacca cagacttggg attgtagatc
     4321 gaaacgccgt ctacacagta accgtcatac cacttctttg tctctgtaaa cggcatcttg
     4381 tactgctcac acagtcggcg gacttcctgt tctgtaaagc cggtaaactc ttcaatggga
     4441 gaggcatccg tcatagaata ttcgtaaaaa gcattgatgg cagagtgctc tccgtatttt
     4501 ttgatgggta aaattcccgt catgtaggca agggcaatgt a
//
LOCUS       DAWBGR010000184         4540 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4547_length_4540_cov_9.296098, whole genome shotgun sequence.
ACCESSION   DAWBGR010000184 DAWBGR010000000
VERSION     DAWBGR010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4547_length_4540_cov_9.296098"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1226 a         1086 c          816 g         1412 t
ORIGIN      
        1 aaaaaagaac attcaggcaa atcaacagct aaacttgcac ctccagctgt ttcacttgct
       61 tgaatgttct tttcattttg gttgcgggag ccagatttga actgacgacc ttcgggttat
      121 gagcccgacg agctaccgag ctgctccatc ccgcgatata ctttcgtccg tacctctcgt
      181 accgacttaa ttagtatacc acattttgcg tcgcatgtca accatttttc cgatttcaaa
      241 aaataaaaaa gttcaacagc atcaaattta taaatataag gtgcaatcaa cttgtaaagt
      301 gcaattcagc aattatataa gcccaaaacg caatcgaaaa agttgtatct ttcaaattac
      361 cattgctcct gcaaaagcaa aattcaggcg tatccgcata aaaaccaacg ctctgcggca
      421 gaatcctatg ccaacacgcc caaatcacac gcataatttc gtcgtttttc caaattattg
      481 ttatgccatg atcgcattct tatagcgaat caagtccaaa taaatctcct gctgtttctt
      541 gtcaatagca tactgaatat acttgtccac gtttttcttc gtaacaaaat ccgtctttcc
      601 cagaagcacc tgaatcagct gcaaattacc gttgtcaatc agcatcttca cgcccttggt
      661 aagcatcttt ttcacatacg ccttagcttc tgcgttgtcg tgcaagtcga tcatttctac
      721 agccatctga aatttcatac acagcggcat tttcacctct gtgtccattt ttccggttcg
      781 cagcatttct ttcaccgcaa caatatcttt ttccacttca atataatagc caacaatcag
      841 gaacttttca tcagaaatcg caattttaat attggtcaga ctttcacacc cccaaaacgc
      901 cgcttctaca atattgatta cacgcttcgg aatcataatg ctggtcaaac ttttacaaca
      961 ggcgaatgcc caatttccaa tactgttcaa actgtcggga atcttaacac tagtcagcct
     1021 ttcgcagcta ttccatacac cacttccaat actagtcaca ccatctggaa ttgcgatact
     1081 gattagatta gtacatttat aaaacgtcca atctccaatg ctagtcacat gatttggaat
     1141 tgtgatattg gtcaaactta tgcaatcaac aaatacacac ttccaatact agtcacactg
     1201 tcaggaatcg taacgctggt caaactttta caactatgaa acatataatc tccaatagtg
     1261 gtcacgccgt tcggaattac aatacttgtc aggcttgtac actcacaaaa cgcatgatcc
     1321 tcaatactag tcacactatc cggcattgtg acactagtca gaccttcaca cctcgaaaac
     1381 gcccaatcac caatactagt caccccgtcc ggaatcacaa catctccgcc tgcaccagta
     1441 tactttacca gtacaccatt ttcaatttca aagtcattgt tactgctcat ttgcattttt
     1501 cctttctgta taaaacgacg gtatcacatc aattataaac tgtcttaaaa taaatgtcaa
     1561 gagtaattcc atagattgta aaacagtttt gtttcatttg atgctaatat tatgccactt
     1621 tagtttttca aataaaatag aactatcaaa attctgccga aaaaacaaaa aaacagggca
     1681 aaaacactgc cctgcttttc gtttgcctgc agatgaatca agacggttcc tgtgtaaaat
     1741 cggtgatttc ctccacgatt tgtgcaattt tgaccatttg ttccggcgac agcccattca
     1801 tagcaacctt gatgcgatga aaagcgtctt cttcccccac ttccgtatta gaatcaatgt
     1861 ccagcaattt cgggatcgac actttcagcc cgttggcaat cttcgaaacg gtttcaatcg
     1921 tggggcattt ttccccgcgt tccaccttgc cgatataagc ggggtgaatt tctgatgcaa
     1981 aagccagttc ttcctgacta aggtgctgcc gggtgcggaa ctgacggatt cgttctccga
     2041 cagcagcggc gacgttggtt ttgttcttca taatggaaat cctctgtttc tgctctaaag
     2101 tattctatat ttctatttta aagggtatct gaaacagatt caaatattta tagtgccttt
     2161 gtcaataaaa aactgagcca cctcgctaat aaaaaagtga gccagttgtg ataaaaaaat
     2221 tttgagccac taatcgtgat tcaaggttga gtcgatgcgg taagacgagc tgcagttcat
     2281 gttcagaaca aaggatcgga aggtgaccct gtctatgagt gcagcaagca gcatttcatt
     2341 ttcaaaaagc tgtgtccatt gtgaaaattc aagatttgta cttatgataa cactggctct
     2401 ttctgaacgt tctgatatga tctgaaaaag catctctgac tgatacctgt taaaagtcag
     2461 gtaactgagt tcatcgatga tcagaagatc tatcttactg agactctttt caagtttgga
     2521 aagcctgttc atctgaactg cttctgcaag ctcagcagca agattgacag ccgtatagaa
     2581 ttttacccga taccccgcct cacaggcgtt catgccaagt gcaatcgaaa gatgtgtctt
     2641 tccactcccg ggatttccga tcatgacaat gttttgacgc tgctttatga aatcacagga
     2701 agcgagctgc tgtatggtgc ttttactgac gttttcaagt cttgccatat caaacgcatc
     2761 cagcgttttc agtgttggga attttgcagt ttttatcctg cgtttctgac ccgctgctga
     2821 acgttctgca agctcctgtt tcatcagctc tatcagaaat tgtgcataac catcgtcagc
     2881 agaaagctgt cgtatgactt tttcatagtt attgaaagtc ggcagtctga gttgttttgc
     2941 atagagcctg atcatttctt tctgtacgct gttcataccg catcaccgcc tttcagaagc
     3001 gaatcatatc gtttaagatc cggcggtacg atcccgatgg aggattctat ctgtattttt
     3061 cttaccggac ttccgatcac tcctgcatac agcgtgttgt aggaatctgc ataaggcaag
     3121 tgtttcaata ccgcatttcc gatatccagg tacagccgca gcaccttgac aatttctttg
     3181 ggactgctca gtttcatcaa aaactgataa agttcggtcg gaacagtttt cttgatcggt
     3241 gcagcattgt aaacacttct cggacgcttt tcgatcaatc cgatgtaatg ctctaaacga
     3301 taatgggtct ggttatgacg gtaatcacga tcatattttg caatgatctt accgcggaac
     3361 agcattatga cctgattgcc gcttcctttg accgttatca tcttggaact gtactgatac
     3421 ggcactgcgt atttgttgtg atcaaatctc acaagtgaga aatcatccgc tttgatctga
     3481 agtgtatttg atgtatcata gcggtacggc ggtaaaggga tccaatgctc cttacagttt
     3541 tcagccattt cacctaccgt cagatctttt ccgggtatct tatgatttct gtacacagcg
     3601 caatattcca gaagcttttc attcagctca tctatggtcg atatgttagg gacaggcacg
     3661 aagaaatttc ttctgacaag tcctaccaga ccttctacaa gccccttttc atgaccctgt
     3721 gccggattgc agaatactgg tttgaaggaa tagtgtgccg acagggaaag atatttgtct
     3781 gttgcttttg catgacatcc aaatccttct tttacagcaa cacgagcatt atcaaaaatg
     3841 atcttctgcg gcgttccgcc aaaatgttca agtcctttta cgatcccttc cagaaagctt
     3901 tcttcattct gccggtaaaa agcggaaaca aagatatccc cgctgtggca ttcacgcata
     3961 caccatattt ggattttctg cctaatcccg ccaagtatta cagttgcttc accccaatcc
     4021 acctgaacgg cttcggctgg gtcataggac aatggtacaa acacattgct caccttgcct
     4081 ttgagttctg ccaccgtccg tcttacagaa gcttcacagc catcaaaacc acattcggca
     4141 accaggcgtg tgtatatctt atgtgctgta tgctgctgct tccgcggtgc tttctggtca
     4201 gaatccaggc acttcatgat aaatgctttg acatcatcgg tgatgatgtc attttttctg
     4261 ccgctgccag gtttgcgttc ccatggaact gttcgtcctt cccagtattt ctttactgtg
     4321 tttcgtgaga tccccagtat ctttgctaca ttacgctggc tgaagtctgt atgctctcga
     4381 taatatctga tcttttcata gatctccatt gctattgcca ctccttattc ctcctgtata
     4441 caagttcttt ctcttatgat acaggatttt tattatttgt ggctcacttt tttattatca
     4501 tttttccctc agctggctca gttttatttt agcagatata
//
LOCUS       DAWBGR010000185         4537 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4552_length_4537_cov_4.688532, whole genome shotgun sequence.
ACCESSION   DAWBGR010000185 DAWBGR010000000
VERSION     DAWBGR010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4552_length_4537_cov_4.688532"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2829..2838
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1032 a         1196 c         1189 g         1110 t
ORIGIN      
        1 cgcagggcgt gccttgtgct gagctggcag tgcgtagcac tgactgaggg gtttctactt
       61 atgccgaaaa cgtcataaaa agattttacg aaaaaacctc tccaccgcct aacggcggtc
      121 cccctcccct ttcaggggag gctatcatga aaattgattt gcgtgttttt cacctgctcc
      181 ggtttgacaa tttccgcaaa acatgataca atagaagctg ttgttccttt cggaacactc
      241 catgcagaaa gggttgtttt atgaaattcc tgaaaaaata ttataggctg atcggtacgc
      301 tgatcaccgt ggtggcattc gtctttgtca tcaagaagat cgtgactatg gacgtggact
      361 ggtcgatgtt cgcttccggc aaaccgctgg gcatcattgc cggctgtgtg ctggtacaga
      421 ctgccattat cttcttcatg tccaccccgt gggtgcagtt tgtgcgaatc ctgtccggca
      481 agaaaatcgc catgaaagac gctctgccgg tatacaccaa gtgcaatctg atgaaatacg
      541 tgccggggaa tgtgttccag tacgtggggc gaaaccagct tgccgcagac ctgcacatca
      601 gccacgtgga cgttgcctgt gccaccgttc tggaaattct gtgttcgctg gtggctccgc
      661 tggtgtggat cctgctgctc atgggaaaag acatggtggg actgatccgc acctatgaga
      721 agaatttcct gctggtgctg ggcatcggtg ttgccgtact ggtgctggcg tttttcctgc
      781 tccgctggaa gtttcgggag ccgctgcggc ggtatttcga aaagtaccgc aaactgctga
      841 accggaaaat tttgctgcgg gtcgttggcg ttttcctgct ctacgtgctg caatacctgt
      901 tctccgccac catgtatgcc gtgccggcgt tcctgatgtt cgacgttccg cgggcacaga
      961 tggggctgtt tctgggaacc tacctgttct cgtgggtcat tggcttcatc acccccggtg
     1021 ctccgggcgg catcggtgtc cgcgaagcag tcatggtgct cacatgcagc acatttctgg
     1081 acaccaacac cattatgctc tacgccgtta ccatgcgaat catctccaca ttcggcgacg
     1141 tgctggcgtt cttcctgggc tggctgctgc atctcatctg gaagcggcaa aagaccaccg
     1201 cgtaacacta ctattataat ataacacttt tcacagcaaa catacaaaaa agctgtcggc
     1261 ggaatcattt cccaccggca gcttttctct gtacagttcc atctgtcttg tcaaagttca
     1321 ctgtgcactg ctctgtgtat tctgcgtatt ctgtgtgttc gcagcttcat tggcagttgc
     1381 catgtgcata ttgaaaaatt ccggatacag ccggcgtgaa acctcaaagg tattttccga
     1441 cacccgcttt gccatataca ggtccatgtg tacgccatgg gcataaatac gatctacttc
     1501 tccgatcgca tgtgctccgg aatccagttc ctttacatat acaatatctc ccacctgtgt
     1561 attcggggta acggcagaaa ccgtataagt catcttctct gtcagcatcg tgatctgctc
     1621 ctttccgtat cctgtgggaa gccgccgcga aatgccagtc gcacaaaacg cgggctgcac
     1681 tcccctatct tttttatttt actacatttc tctcgtttcg tcaagtgttt ccagcaggtt
     1741 cctgcaaatt tctctgaaaa tccccttgac aaacccttgt tcctatgtta taatacgaat
     1801 atgaaaatca ttttcaattt caaatactgc aattccaacg gacggaagtg agagaatatg
     1861 aaacgaaaac tggctttttt gctgatgctc tgcttcgtgt ggagcttttt tttgaacggc
     1921 tgcggcggaa cgctgccgaa aacagacgac cggctgcaag ttgtctgcac catttttccc
     1981 gaatacgact gggcaagaca gctgacacag ggcgtggaca acgtagacct gacactgctg
     2041 gtgaaaaacg gcacagacct gcacagttat cagccctctg tacgggacat tgccatgatc
     2101 gccagggcag atgtgttctg ctacgtaggc ggcgtttcgg atacatgggt caacgacgtg
     2161 ctggacacca ccgagaaccg caccgcacag ctggtttcca tggcagaagc cgctgacgct
     2221 gacgaggaag tgctggcaga gggcatgcag gcagaacatg aacacgaaca tgaacacgac
     2281 caccacgatg acgaaagcga ttcccacgag gagccgccgg aactggacga acatgtgtgg
     2341 ctgtccctgc ggcgggctgc actggcatgc actgccatga aagaggcgtt gtgcaaggca
     2401 gatcccgcac atgaggacat ctacaaccag aactgcactg actatctcca gaaattacag
     2461 gagctggaca gcgactttac cgaaatgact tcgcaggcgg cacgggatac cattgtggtg
     2521 gcagaccgct ttccgttccg gtatctggcg gaggattacg gactgactta ttacgccgca
     2581 tttccgggct gctccgcaga aacggaagca agctttgaaa ccattgtatt cctatcggaa
     2641 aaggtagaat ccctgcacct gccctgtgtg ctggtcaccg agggcagcaa ttcctcactg
     2701 gcgaaaacca ttctggaaaa tgccggctca gacgggaaaa ttctgacgct gcaatctcta
     2761 caggcagtat cgcaaaagga gatcgatacc ggcgttacct atctgtcaaa aatgcaggaa
     2821 aattatgann nnnnnnnngt gctgaaacag gcactgaact cttgaggggg cgtgaaaatc
     2881 tatggcacaa ctcattggaa aagatgtcac attgggctat gagggcaatg tggtcatcga
     2941 acacctgaac ttctccgtgc agacgggaga ttacctctat attgtagggg aaaacggctc
     3001 cggcaaaagt acgctgatga aagcgattct ggggctgaaa tcccctatgg cgggaaccat
     3061 taccaccggc gacggattac agccggacga gatcggctat ctgccccagc agacggcagt
     3121 acagcgggac tttcccgcat cggtgctgga aatcgtccgt tccggctgcc tgaatcacag
     3181 cggcttccgt ccgttttata ccaagcagga aaaacagctg gcactggaca acatggaacg
     3241 gctgggcatc acgcatctgg caaagcggtg ctatcgggaa ctttccggcg gccagcagca
     3301 gcgggtgctt ctggcacggg ctctgggggc aactcagaaa atgctggtac tggacgaacc
     3361 tgtcacgggg ctggatccca aggcacagct ggaactgtat gagctgatcg cccagctgaa
     3421 ccggaaagac ggcatcacca ttctcatggt ttcccacgac atggaagccg ctgtaaaata
     3481 cgcctcccac gtgctgcaca tcagcaaaac gcccctgttt ttcggcacca aagaggacta
     3541 tctccgcagc aagatcggac agacatacac aggagggaca gaagcatgag tatctgggaa
     3601 cagctgacag ggtatctgtc attttcattt gtccggtacg ccctgatcgc aggggtactg
     3661 atcgctctgt gttcctccct gctgggagtt acgctggtgc tgaaacggtt ttccgtcatc
     3721 ggggacggac tttctcacgt ggcattcggg gcacttgcca ttgccacggt gctgcggctc
     3781 agcaacaaca tgctgctggt aatgccggtc acagtggtct gtgcagtgat cttgctctgt
     3841 gccaaccaga atgccaagat caaaggcgat gctgccattg ccatgctgtc tgtcagtgcg
     3901 ttggcaatcg gctatctgat acagaacttg ttctcctcct ccgccaacat ctccggcgat
     3961 gtgtgcagca cgctgtttgg ctccacgctg atcctgacgc tttcgaaaag cgatgtctgg
     4021 ctctgcgtgg gcatgtctgt ggtcgtcatg gcgattttcc tgttgttcta ccacaagatt
     4081 ttcgccgtca cctttgacga aacctttgcc gcagccacgg ggatccgtgc cggcatgtac
     4141 aacctgctga tcgcagtagt cacggcagtc atcattgtcc ttgccatgaa tctggtaggt
     4201 tctctgctga cttccgcact gatcgttttt ccggcactgt ccgccatgcg gctgttccgg
     4261 agttttcgcg gcgtgatttt ctgtgcagcg gtgatctccg tcgtctgtgc tgtagtggga
     4321 ctgctgatct cgatactggc ttccacgccg gtgggggcaa ccattgtcat tgccgatctg
     4381 gtagcatttc tgggctgctg cggcacaggg gcagtgctcc ggagaaaata aaagaaacag
     4441 ccataaaaca aaagcggctt actgaaaaag taagccgctt ttttgtttag gcaataccgc
     4501 acaaagattg tgcgacagat gcgtcgtcaa atttttc
//
LOCUS       DAWBGR010000186         4492 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4605_length_4492_cov_2.723011, whole genome shotgun sequence.
ACCESSION   DAWBGR010000186 DAWBGR010000000
VERSION     DAWBGR010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4605_length_4492_cov_2.723011"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1287 a          916 c         1072 g         1217 t
ORIGIN      
        1 gatcgacagc cgtgcattct ccaatttttg tggaatcaac tcaccgagcg aagtcccaaa
       61 cggtgatacg agatgtagat ttatcaattt tttcctcgtt tgcgttttga tattttgttt
      121 tacgatattc gttcacttta cttttcgcag tttccagatt ttcaaaatcc tttctaattt
      181 cacctactgt tgtattttta ttatatccac aaaatgaaat ttttatatct tttttgtctt
      241 cggccattat tttctctcct taaaatttgt gcagtattgc cttgaatctc atctgccctc
      301 ttgcttgttc ttcaaaaaag ccagacaggc atttgcagtt ccgtgggtga agccttgctt
      361 gtcgatcaac atggtgaaat cctgacgggc aaaatataga acgatccacc gtttatcgca
      421 tcgaatccat gtcaggttct catattccag ctggtatttt tccaaggagg aagtactcat
      481 catgcggata cattccggta aaaaagtgta ctgattgact gcctgcagat ccagtttttt
      541 ccttgtgcgg cgaatgcggt cttgtatctg ttttttcggt agatgccaca tccaaaaggc
      601 ggttgccagc aacagtaaaa ataagaaatc caaaaatcca aggtgaaagt ctgccgtatc
      661 gccaatttcc aacaaaattc cccgatagat cagccgttcc aaaaacggta ttgccagcag
      721 aattccaatg gcacggcaga taatgcagtg ctgtttcttc tgcatctgta aaattgcagc
      781 gtatccgtct tctgtaatgc gtgttgtgtt ctcaaagtag tccaaaacgg cacctcctgt
      841 ttctgcgata tttttattat accatgcggg aaacggtttg taaagttgtt tcggaaaaaa
      901 gtcgaattat gtcttgacga aactggggct tctatggtac aataaaatat acacaaaact
      961 aaatttttat attggaggaa aactgtatga agttcacaaa aatcatagct gctgcactgg
     1021 cactgctctg cggttctgcc atgctgccga aagccactgt cggtgcggaa gaagtactct
     1081 tggattctgg cattgattac acggaatcta cggaaacact tgggaatccg ggagccggat
     1141 acagttctgg tgttgcggtc agctgcaagc ctaacgacac caaggtgtac aacccgactg
     1201 ctagtctggt ggtgctcttt gtggacattg gcggcttttc cagcggagca aacggaacga
     1261 ctgacgacag cggcaattat accgctggta cggactatga tctggacgaa acgttcttta
     1321 caaacttccg aaaaacattg gaaaactgcc gcaaaaacgg ctgtaccatt ggtattcgtt
     1381 ttcggtatga tgcgaatggt gtccgcaatc ctgaaccggc tacttttgag cagatgtgca
     1441 agcatatcga acaaatacgg aaagacggtt ttctggaaga ttacaaggac attattgcct
     1501 atgtagaaag cggctttgtg ggttgctacg gcgaacaatg gggcggaaaa tactgctctt
     1561 tggaagacaa ggcgaagctg ctggatctgt tgttagatgt ggttcctgac ccgattccgg
     1621 taactgtaag aacaccgaat atctttgcca aatgggccgg cattgaggaa tcggaactgg
     1681 gagaatatgt gctggaaccg aacagcaagg cttcccgtgt gggattgtac aacgacggtt
     1741 acatgggttc tgattccgac ctaggaacgt ttcacaatcg tgaacgagat ttgaagtggc
     1801 tgcggcagca gacatatacc tcctattatg gcggagagtt ttccggaaat ctggactttg
     1861 ccaaacagta cgatacatat cttccggaaa atgcggtgcc ggaaatgtat tattcgcact
     1921 tgagttatat caattcgaat atttttaaac tgtatcagga ttacactttc ggaaaagaat
     1981 atgatgtaga gaacgtagac aacagtgctt actatgggga aacggttttc aaattcatac
     2041 gggatcacat tgggtatcgt tttgtgctgc gggattccga tttgagcgaa agcgtggagc
     2101 aaaacggtat tttgaaagtt tgcaccaaaa ttgagaatac cggttttgcc aacccgatta
     2161 tgcagcaaaa ggcagagatc attctggaaa aggacgggaa ttatatccgg acagctgttg
     2221 atgccaatgc aaaccagtgg tattcctgca caactgtttc tccggaattc gacctgaagc
     2281 ttcccgcagg cattgaagcc ggcaagtgga atgtctatct gaaactttcc acgggagaaa
     2341 acgacatcag cgaaactaac gttcgttcta tacagtttgc caatcatgat acctggaact
     2401 ctgctctggg tgccaattat ctgggtagct tttctgcaac ggaatctgat aagccgcaga
     2461 cagacaacga cttttatcag atcaacacag aacaggaact cccccattct gacggctctg
     2521 tctacactta tcagaacctt tctgttgtag acggtatctc cagcaatgac acggaatatg
     2581 cggaggacaa gtgcactgtc acagacgata atggcaatca gctgtacatc actaacaacg
     2641 accaatattt atacgtcttt gctgatttga catatgatgc tgcttctccg gtgtacaacc
     2701 tttcattcca gaacaaaaca agtggcaaac ggtgctggat ctactatcag ggaaacggat
     2761 ttgtgtattt caacaatggt gttcccagcg gatgtgtcca gaagcacggc ggcagaaaag
     2821 tcgaatttcg gatccctttg ggagaactgg tagatttgaa ggctggaact gaaatttcca
     2881 acatcagtgt gaccattcag gatcaggcaa attcgtgggt aaatgctggt tctgtcaacg
     2941 cggacagcta tattttacag gacaattttg atgtgtattc cgcaaagcag acagtttacc
     3001 ttgcagaaaa ggacaccatg ctgctgacac cggaaacctc tggaacgggg ctttcttacc
     3061 agtggtacca caatggagaa gcaattgacg gagcaacgga aaaagcctat accgttacag
     3121 caaatacgca ggaggactgc ggggtttatt ctgttgcagt ttcctctccg agtggcacag
     3181 tgaaaattgt ggaattgtgt gatgtcaagg gcatcatttc tcagacaatt tcgggtgatg
     3241 tgaatcagga cggcgttgtc aacacggcag atatagtggt tttgcagaaa tatttgttgg
     3301 gaatcgatat actgacagag gaacaggcag aactggctga tacaacggca gacggcacgg
     3361 tgaatggttt agatctggga ttgctgcgga gcatgatcgg ataaacaaga agtaaggcaa
     3421 tatatcgcag ctataggcaa aagaagtcgg aacagagtat ggaatatact ctgttccgac
     3481 tttcttcttc cagaaactgt ttaaaaaatt aaccaggaat tattttactg cttttctttg
     3541 cgatttgctt atggtataat atgaataagg ataaattacc agattcgatt ccgcatattg
     3601 ggacatccag ccaaaacaag aatttcgctt gtcacaaaat cattgtttta tgcgttaaat
     3661 tctatgagaa ctgttttggc ttgaaaatat gcggaatcga tttggctgtc caaatcaatt
     3721 acaagggaga tcagaggcgt ttatgaaaaa gaagcatctt cagaagcaaa ttcttgctgc
     3781 aatgctgact ggcgtaattg tggtaaacac ttgcccgatt gtcgctatgg cagcagaaac
     3841 tatgccgccg gaaacggaaa tggtgcagga agcaacagaa gaaacgactg caacagaagc
     3901 ggcagaaatc ccagaaactc cgggagcaga agtgcaaacg gaactttccg gttcggaagc
     3961 tgtggaagtt acaaccgcag aagcggaaac agaaatggct gcggcagatg ttgaaactgg
     4021 aacggaagaa attccgcaga cggatgcacc ggaggatatc gcagaagatt ctgtcacggc
     4081 atttgtcaac cggctgtaca acgtgtgctt ggaacgggaa ccggattctg ccggcttaaa
     4141 aaattggcat gacaggatca gcagcgacag catttctgcg gtagatgctg ttaagggatt
     4201 tttgaacagc aaggaatatc agaacagaca gctcagtgat gaagtgtata tcgctaattt
     4261 gtatcaggtt ttcttgaatc gaaccggaag cagttcggaa attcagcact ggctgattat
     4321 ttatcagcag ggcgtttcta aaaactatct gatgcatggc ttttccaatt ccactgaatt
     4381 cacaaatctg tgtgcttctt acggcgtgac acgcggcagt attgcattga cagaagaacg
     4441 tgacaagtat ccgaatgttg cgaaaatggt tgtgaactgt tatgctgttc tg
//
LOCUS       DAWBGR010000187         4491 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4606_length_4491_cov_3.854824, whole genome shotgun sequence.
ACCESSION   DAWBGR010000187 DAWBGR010000000
VERSION     DAWBGR010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4606_length_4491_cov_3.854824"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4290..4320
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1061 a         1168 c         1140 g         1091 t
ORIGIN      
        1 gctttgcaga gtgcggcagg gtgtcagttc cagctgtacc ccgtttgcct gtgccagttc
       61 ctgtacagtt ttgcaatgct ttgcgtaaaa gcgaaactga tatgtgtttc cctgacaatg
      121 ctgtttccgg cagcacaccc ccgctttctg catggcgttg ataaatgcat acaattttcc
      181 gccggctgcc gccgccttat actctccccg cagatgctgt tccattgcgt gccccctttg
      241 gtgtcagtgt gatcgactgg atctttccgt gaatccgcag gctttccggt gaacggctgt
      301 ccgtctgcaa ttcgctcccc cagatctcca gcaccagatc tgtggtttgc aggacaattc
      361 gaatgtcatt gtattccagt atcctgcggc aattctcaat gaaaatttcc cgatcgctgt
      421 gcatttccag atgtgtttcc agctgcaaca cacgtcgtcc tgcctgaatc agccggttct
      481 ccatgaacat cacctcgaca aagtctatgc gaatccataa catattatgt gtgccgctat
      541 acataccatg gaacaggagg gatcgtttat gactgcacat ctttttcgcc tgcggtggta
      601 taccgttttt ttcttattgg gactgttggt gctctgcacc tgtttcgccg gacagataca
      661 ggcagaactg caaactgcgg tgaaccgctg tctgacagtc attatcccgt cgttgtatgc
      721 catgatgatt ctgtcccagc tgtttctgga aagcggtgcc tggcaggcgt tggcacggcc
      781 gcttcgccgg ttcagcaccg ccttgttcgg gctgccggaa tcttattttg ccctgttcct
      841 gctcagccag ttcgccggtt atccggtggg agcaagcatg ctctgtacac tgacaaaaca
      901 gggtgtccta tccaaggaag atgcgtccag actgctctgt gtctgctacg gcggcggacc
      961 ggcgtttttg ttgggactgc tgggaacggt ttcctgccgg agaacctgct ttctgctgat
     1021 attttctgcc aatctgttcg ccaatctcct gctctgcttt ctgttatttc accggaagcc
     1081 gatttcgccg ccggtgaatg ggaacaatgc aatagccccg ttttccacac aaatgctgac
     1141 gtttgccgtc acctccgccg gacggacact gttaaaactc tgcggcatga tcctgtgttt
     1201 tgcggcactg actggcatat tccaggcggc aggactgttc cgttgctgca ctgccctgat
     1261 gcagcaactg ggagtgtcat ttccgctagc agatctggtg cggagcatac tggagatctc
     1321 caactgcgtg gaactgccgc tgccgattcc cctgcggatt ccggtgctgg caggactgct
     1381 gtcgtttggc ggtgtctgcg tgctgctgca aatttgtgcc gtcacaggga aaaccatttc
     1441 cgtcggaaag ctggtctggg cacggggctt taccggactg ctctccggcg gcatctgtgc
     1501 tgctgtactg catttttccc cgattgctgt atcggagcag gtgctgacaa catcagcagc
     1561 cccgcaaacg gctgctcacc cagtttttcc gattctgatg ctgctgtgca tgatgctgct
     1621 ggtctttcgg gaggagcaga agctgcagca atctccgtgc agaaaaaaga atgcctcata
     1681 aatatgcggt gttgcccaaa gaaacactgc ataaaaattt tctagaaatc acttgacttt
     1741 ttggacagaa catactataa taaaagagta tgaatgtgct cgctttcaga gcattgcaca
     1801 aaatttctgc acagttacag aaaggggata ttgatccatg aaactcaaaa agaaacttgc
     1861 tgcagctgcc gtttccggca tgatgctggc aacgtgtctg tcttccgctg ctccggtggt
     1921 acagacaagc ctgtccgctg ctgccgcaga tgataataac gacgactggc tgcacgcaga
     1981 gggcagcaag ctttacgatg caaacggcaa tcaggtctgg ctgactggtg caaactggtt
     2041 cggcttcaac tgcacggaaa actgtgcaca cggtctgtac gcaactgaca ttgatgcatt
     2101 cctgaaaacc gtcggcgacc acggtatcaa cctgattcgt atgccgatct ccacggaact
     2161 tctggtttcg tggatgcgtg gcacaccgga tcctgtcagc tccgttaatg ccggctatga
     2221 gccgccgacc gatcaggtgg gcgaggacgg cactgtcacc aaggctggta cttatgtcaa
     2281 caccaacaag gactttgtca aggaagacgg caagacgctg aaaaacagta tggagatctt
     2341 cgacgtcatc atgcagaaga tgaaggacta cggtctgaaa gtcatgatcg acgtgcacag
     2401 ccccgcagag cacaactccg gtcacaacta caacctgtgg tattatactt ctgccgcttc
     2461 tgacggtgac accatggcta ttggtgcggc ttccggtgaa aagatcacat gggatatgtg
     2521 gaaggattct gtcacatggc tggctgaaaa gtacagcaat gacgatacca tcatcggcta
     2581 cgacctgaaa aatgaaccgc acggcaaacg cggctacagc ggaaacaagt gccctgaaga
     2641 tattgccaag tgggacaatt ctaccgacca gaacaactgg gcatattctg cgacagaatg
     2701 cggcaactct attctggatg tgaaccccaa cgctttgatc tttattgagg gtgtggaaca
     2761 gtatccgaaa acagaaaagg gctacaccta tgatactgcg gacatctggc aggctccggc
     2821 agaccagtcc ccgtggtatg gtgcatggtg gggcggcaac ctccgcggtg tcaaggatta
     2881 tccgatcgac ttcggctccg gttccagaaa cagccagatc gtatattctc cccacgacta
     2941 cggtccgtcg gtttataacc agacatggtt tgacaaggac ttcaccacgc agaccctgct
     3001 ggacgactac tggtatgaca catgggcata catcaacgat cagaacatcg ctcccctgct
     3061 gatcggcgaa tggggcggtc acatggacgg cggcaaaaac cagaagtgga tggaactgct
     3121 ccgtgattat atgatcaaca accacatcaa ccacactttc tggtgcctga acccgaactc
     3181 cggcgacacc ggcggtctgc tgggctatga cttctccaca tgggacgatg acaagtatag
     3241 tctgtttgaa ccgtccctct ggcagacaca ggaaagcggc aagtacatca gtcttgacca
     3301 caagcgtgca ctgggtaccg gctccaccag tatttccctg agtgattatt acagctctta
     3361 cgatgcttcg gagggcagca atattgacgg cggcagcaag ggcagccacg gcgataccaa
     3421 gccgatacag tcgggcacga ccagtgccgg cacaacgacc actacaacta ccacaccgga
     3481 aacggttgtc ggcgatatta cgctggacgg tacagtgaac gtcaaggaca tgatcctgct
     3541 gatccagtat ctgtccggag aaaaggtaga cagcaagggc aaggacttca aggctggcga
     3601 tgtgaacggc gacggcacac tgaacggtct ggatctggct ctgtaccggc aggtactgtc
     3661 aaaggcaatc agcggattcc cgagctaagg cggtggactg atgaaacgtg acgattattt
     3721 gtcgtgggac gaatatttca tgggcattgc cctgctctcg gcacagcgaa gcaaagacag
     3781 caacacacag gtaggtgcct gcattgtcaa tcaggaacac aaaatcgttt ctgtgggcta
     3841 caacggcatg cctaccggct gcaacgatga cgacatgccg tgggaacggg agggtgattt
     3901 cctcaacacg aaatatccct ttgtctgcca tgcggaactg aacgccattc tgaaccgcag
     3961 tgccggcagt ctacaggggt gcagtttgta tgttaccctg ttcccctgca atgaatgtgc
     4021 caaggcgatc attcaaagcg gcattcggga aatcatctac tacgacaaca agtatgcgga
     4081 ttctgatgcc acgaaagcct cccagagaat gtttctgctg actggtgtga cctgccgacc
     4141 gtatcaccac acggaacgac agatgacatt ggtgctctaa ggaaacgcta cataaaacaa
     4201 agatttccag acaccctctc cttccggtat ttctgaagtg agagggtgtc tttttctgtc
     4261 catgtgcagc aacatttttt cttgcaaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 gttcttaaca atattttttg tgaaatatgc ctaatttcaa aattttctgg tttttttcaa
     4381 ttttcctttg tgttttgtcg gaaaatatgc tataataaaa tagatcataa acttaggaaa
     4441 acactgcata aagcccgcct gacggctttg cacgcaatct gcttgcagat t
//
LOCUS       DAWBGR010000188         4439 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4671_length_4439_cov_2.848084, whole genome shotgun sequence.
ACCESSION   DAWBGR010000188 DAWBGR010000000
VERSION     DAWBGR010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4671_length_4439_cov_2.848084"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1434 a          861 c          946 g         1198 t
ORIGIN      
        1 gtatcgtgta catcccgacc aacagctttt atcaggtgct ttcggcagaa gcctattcca
       61 agcatggttt caacattcac ggagttgtgt ttgatgaact gcatacgcag tcgtacccaa
      121 ctaagggaac catattgttt ataatttatt tacattcaaa aattaagaca aacagacagg
      181 cttaaatttg caaagaaaat tgaatagata acgaaaagcg ttcttcatat atcagtgagg
      241 aacgcttttt ctatttattc ggaggttatt atggcaaatg aatcaaattc atcggttgtc
      301 agagtgcata agaacaccga cttcacgatc atgagcaatc atcatttgag aaatcagaaa
      361 ctcagtctga aagcaatcgg gctgatgtcc aaaatactgg gtctgccctg tgactggaac
      421 tattccattg ccggactggt gaagatctgc aaagagggtg aaacggcagt tcgtgctgcc
      481 ctccatgaac tgatcgacga gcggtatgtg tatttggaga agcttccgcc gaactattcc
      541 aaaagcggac ggtttgaata tgtttatcac atctatgaaa ttccatatga gaatattcct
      601 gatggtctgg aatgtccgga actgtttctc aaaaagcagt ctgaaacaaa tcaaaatgca
      661 gattctccag atacagaaaa acaagatgca gaaaacctgt atcttgaaaa tcaggatata
      721 gaaaaccagg gacaattaaa tacttacata tcaagtatga aagaatcaaa tatgaaagaa
      781 ttaaatgaaa cggcttctgc tccgccgaag aaaaaggcgg ataagaagaa atatgcagaa
      841 gctgtcacca tgacggaaga agaatatcag aagctgtcgg aacagcattc caaggcattt
      901 gtggataagt gcattgagat tctcaacaac tacaagcttt ccagtggaaa acgatacaag
      961 tcagattatc acaccattct aaactgggtc atcgaaagag tttccaagga ttatcctcag
     1021 ctggacaaat caactgcaca aagttcggct tacgatgtca atgtcaatcc atttgaccag
     1081 tttgtgaggt aagaaaatgt ttatcaatac ggaattactg gagtttatcc gcttgaaaaa
     1141 tcccctttct tctgttatcg ggaaatacga agtgataaca gatgcttgct gtaaatgccc
     1201 attctgtcac agcaaaacca attctctgaa tcttttccat gatgagatct acacctgttt
     1261 tcactgcggt gaaagtggtg atgtatttgg atttgtcagc aaaattgaaa atctttcgtt
     1321 tgcggaaaca gttcgtaaaa tggcgaaagc atcgggaatt tatacactgg aagaattaga
     1381 acagaagaat attttccgct tttgggacag atttctgatc ctctgtgagc attatcacat
     1441 tgcagcagac agcatattgc aggaatattc ttcggagctg acaaaagaat atcttctcag
     1501 tctgaaatat ggcaaagaac cggatactgc tccgctggaa aatatcatgc agatgaaata
     1561 cggcatatcg aaagaatttt ggaatgctgc tcctgagcat attatggagt atctgacaac
     1621 agaaaggaac cacaatgaat gatacattga aaaacatttt tcagacactc tctgaaagtg
     1681 cagaggaaaa cttcgtaaag cacaaaaatg ccaatgacta tctcgctaag gacggtttgt
     1741 ggcactgcgg aaaatgcggt acagcaaagc agttccgact gcctgagcga tttatgaaaa
     1801 tgggaatgcc tgaaattgtc ggctgctgct gtgcctgtca gtctgcaaag gaaaaacggg
     1861 agaatgcaaa gcaacagctt gcatctgtca tcagagagaa taaagaaaac gcagatattc
     1921 ccgaacatta tctgtcagct gatatggcaa ttttggaatc tcctgaagcc aaaagaatcg
     1981 gcaggaacta tatcaaaaac ttcgagaaac tgggcagaat cggacttctc ctctatggcg
     2041 atgtaggaac gggcaaaaca tttctggcag cctgcattgc aaatgctctg ctgaatcagg
     2101 gcgtaagcgt aaaatggctg acctctatgc agattgtgga acacagctgc ttctacagcg
     2161 aatcagaata tgccgaatat atccgaagca ttaccgctcc cgacttgctg atcatcgatg
     2221 atctgggagc ggaacgtggc acagattttg cgttggagcg tgttcacagc cttgtggata
     2281 cccgtatctc agccaacaaa ccgatgatcg taaccaccaa tatcgacatt acggatatgg
     2341 gcaattgtgc tgatctgaaa aagaaacgca cgtttgaccg cattatgcct gcaacatttg
     2401 cctttgccat gaaaggcacg tcttaccgta tgaagcaggc acagaaaagc tacaaaacac
     2461 tcaaagatct gctcttgagt gaaaaaacaa ttggaaagga cagaaacaca aatgagaatg
     2521 atcaaaactt atgagggcat gacaatgaac tgcgatgtga ttgcaacaat tcagtcggtt
     2581 ttcatgaata tcgctaccgg cagcatcagc gaaatggtcg atgacagctt cgatccggat
     2641 accctggaat ttgctgtgac tgcatttact acacttggag atgagatagt ccttgcagtt
     2701 tatgcaacgg aagaagaacg tgattatgcc agatacaagc tggaaaactg gcttgtttat
     2761 gatgttggca gctattacac catgacagaa agaaaataac ggaggttttt actatggaaa
     2821 aaatgatcgt aacagagtac ggcagaccga ttatgctgaa caaggtcaag gaatttacac
     2881 agagaaccat gtttcttgca gacgaaagag tgatccccta tgaagtgttc gcactgcttg
     2941 acagcggcga actggtgaac gtcggcaatt ttgatgattt ggataccgct gagatctccc
     3001 agatcattct ggacatcttc acagaagaca aaaaggcggt attcgatgta aatctggaag
     3061 tgttcggaat caaaaagttc cttgaaatgc ttcgatatgt gtcagctgac agcgattctg
     3121 tctatcgaat tttgataaac gaactgaaac agcagctgaa aagcggtgaa ctggatgtca
     3181 gcttttcttg acggctacat acagcttgct catgccattg ttgctgtggc agcagaaagc
     3241 tacaaatata cgctcttagc tgtcaaaaat cataccagaa gcgattctgt tttcaggcga
     3301 aaagaggaac tggaaagctt ttttctgtcg gagtggtttg cactgctgtc cggacttgac
     3361 ggaagatatt ttatacagaa aatgcaggag gtatactatt ttgacaggta aagagttttt
     3421 atggcagtac attatgattg atcggaagat caggagcgtg aagcttcagt tggatagtat
     3481 gacagaggtt gcgaaagaaa acgcaatgag cagtgtcttt cagaatgaaa tcactgattt
     3541 gaagaatgaa ctgaaaaatc tgattttgga atctgccgct gtcaaaaggt gtattcttga
     3601 tcgaattcag atgattgaaa atgacgaacg cagacaaatt ctaatcaaac gctatttaga
     3661 caataaaaca gttgtaacga tttccaaaga gatgttcatg acttatcagg gtatttatta
     3721 tcaccttgaa attggagaaa aagagatcga aaaactcata taaaacagca gctataagtc
     3781 gaaaagcact ctcactttac aaaagtggga gtgcttgttc tatatagata ttatctttgc
     3841 tccagctgtt ccttagcgtt ttcaatagca attgccaatt tttcttccag taattttttc
     3901 ggcggcattt ttgtcaggta ttgtgcaacg tgaatgcttc ccttatctaa ttccatcagt
     3961 tctactgttt cctgtgattt ttctgcacaa agaatcaaag caatcggatc atcttctccc
     4021 tcgcacatct cattcttttt cagccatcgc agatacagct ctacctgccc tttgtgttca
     4081 ggttcaaacg caccaagttt caattcaata agaacaagtc gtctgagcga acgatgatag
     4141 aaaagcaagt ccatgtagta atcttttccg tccagaacga aatgtttctg tcttgccata
     4201 aaagcaaaat cactgcccat ttcaagtata aatcgttcaa gttcagacaa aatcgcattt
     4261 tccaaatctt tttcgctgta tgtatctttc agtccgagaa aatcaagcac ataaggatca
     4321 cggtaaaata aatccaatga catcttatct ttttccataa gaagtgcaat atcattttta
     4381 atcgtttcat caggcttttt ggaaatagcc gtgcgttcat aaagcataga gtttttacg
//
LOCUS       DAWBGR010000189         4360 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4760_length_4360_cov_4.770499, whole genome shotgun sequence.
ACCESSION   DAWBGR010000189 DAWBGR010000000
VERSION     DAWBGR010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4760_length_4360_cov_4.770499"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          841 a         1216 c         1173 g         1130 t
ORIGIN      
        1 gcaatccccc ggcggggcat acaggcggct gctgccgccg taacattacc cttttcacac
       61 ttgaaattgg ggggtaaggt cagcggcgac tcgccgccgc cgcatgctaa tccgatgaag
      121 aactactatt ttcgccgcgg agcgttgcat catcttcaga aatgatgtac cacttctccc
      181 cgatcttgcc cagtgtggga tagaccacat ctgtgccgga gtcattgctg ccggagaagt
      241 tccgcttgac ttccagcgtt actgcgtcgg aaatatcggc accctcattg cccagcattt
      301 tctccatatc ggtattccac tcggatacgc cggcatcgcc ctgtgaggac atgccctgga
      361 tctgataggt caccttgaca tcgtcgccgt aggtttcctt ccattcctgc accgtgttgt
      421 tgcagccctc tgtgaaactc tccacaacgt cgtcctcaga cttgctgtag gttttcatgt
      481 aatacgtcca gtactccggc ggtgccatca gctgagtcag ggtctgtccg tctgcggaga
      541 gcaccgcagt ataataactg ctgagtacgt cggcatagtc tgacgaatcc ggtgcggctt
      601 tctgtgcctt ttgttctttc tgataccaga acagtccgcc gaacgccagc cctgccgctg
      661 ccgctaccat gatgataccg gcaatgatgc ggatatgccg gcgttttctt gccgcctgct
      721 ctgcctgttt ttgatttcgg gtggttttct ttgccattgc tgatcgattc ctccgttacc
      781 agacgttata gccgttattc tggcacacat agtccatgtc ttccattgct ttcatacagc
      841 accagcggct gccgatcttg acgaacgtcg ccttctccga agccttggtg ccgccgtcat
      901 caatggtcag gctgacttct acggctttgc tgatctccac ggtaatgtca taactggtgt
      961 ggagttcgtc ctccagtgcg gcgagctcat cggcatcact gatgtgctgc acatgcccga
     1021 tgtcatagga cattctgccc tctgtgccgt agttttcatt ccagtagtcg aagtagtcgc
     1081 tcatcatgga cttgacttcc cgccgtgtgg tggaataggt gctttccaga tagtccatat
     1141 acgcgtccgg caccagtgcg gtatacatgc tgccgctgga ataatccacc cctttgacat
     1201 agcggaatac cgccagctta tagccccaga aaaagacgta gtacaccacc gccagcagga
     1261 tcagcacaaa cgccacagcg gcggcgacga tccttcgggt gcggcggaac ttcttttcct
     1321 gtgccgcccg ccggagttcc tccggatctt cggcagcgat gccgtcagct ttttccagat
     1381 ctgccggtgc agagagaaaa ggcatggaaa atttttctgc cgtgggggac ggctcttttt
     1441 tcgcctgctc ctctgccgcc ggcttcttgg gacggggcag gatctcaccg cattccggac
     1501 agatcacggc acgttcgggg gcttcatatc cacagtgttc acatttcatt gctgttcttt
     1561 actccttgcg gcaaactgcc attgccctta acctacactc tggcttgcat tatagcaggc
     1621 agttgcataa ctatacattg catcgtacac ataccactgt ccgtcgatct gggtcatggt
     1681 aagataggtg tcgttgcttt catcgttcat ctttacggtc atgctgacat ctacggcagc
     1741 atcgccggca atgccatagg tttccagacc gtcattcagt tctgacagtt cttcatcggc
     1801 aacgtctgtg ccgccggtca catctacaga gagttccggt gtgccgtcct cgccgaagct
     1861 gccggaaacc atttcctcca gcatgctggt cagatagctt tctgcatcgc tctggctgac
     1921 accgtattcg gcatcgatca gttcccacca gctttccggc accagtgtgg acatggtagc
     1981 tgcatctgca tttacaacgg cgttccagta ggtctgtacc atatcgccga attctgcgat
     2041 atacttggca gaagcggcat agtcagaacc gcacagctga tcgaaatcgt ccattgcaga
     2101 caggctgcac cacttgccgt cgatctttac cgtccacaga gaatagtcat cgctgtcgga
     2161 atcgtctgca ccggtgacgg tacaggttgc atcaatgcac acgccggtgg aaactttcag
     2221 accgaacttg tcggtatctt cccgcaccgg atccagctgt gcagagctgc ccatgccggc
     2281 agttacgcca ttctgttcca ttttgtagga caggtcgccg ccgagggtgt cggaataatc
     2341 ctgcatgtac tggttcatcg ctgccacagc atcttcctcg gacaggtcgt aggtatcgga
     2401 aatataatcc cagaagctgt ccggacagag ttctgccagt gcctcgccgt cacagttgat
     2461 gtgggcattc caataggcag cattgttctt ggctgccttg gtacccagat tgacaccgat
     2521 tccgacgatc acgcccacaa cgatcaggat cagcaggatc gggatcagga tcagggcaac
     2581 gatcagaccg gttttcttct tctttttggg cggctgacct accggcggca ccggattcac
     2641 agtgggatca tagggcggca cattggcacc ctgtccctgc ggcatctcaa acggatttgc
     2701 cgctgcattg gggattccgc tgccggcggg ggctgcggtc ggctccggtg ccggtgcagg
     2761 ggtcggtgca ggggtcggtg caggggtcgg tgcaggggtc ggtgcagggg tcggctcggg
     2821 tgccggagca gctgcttccg gtgcagccat gggattcccg caggtcggac agaatttttt
     2881 gccgtcttct acggcagttc cacatttttt acaagtcatg ttttttctcc ttatatcata
     2941 gttggtatta tactagttgg tattgccttg agtcgggttc gggaaatcgg gtttgggaaa
     3001 taatgggctg tttgagaggt ttacttgtgc aaccggaaaa gattacacgg gagaacccct
     3061 ccgccagctt cgctggtttc cctgctctta ttcgccgatt ttcatgccgc aggaagtgca
     3121 gaagcggttg ccgtcgggga caggctttcc gcagttgggg caggtgcgtt ccggtgcagc
     3181 cggctggggc gtttccggta caggctctgc ggctgccggc tggtctgtca tttcgtgacc
     3241 gcagcgggta cagaaatgat tctcctccgg aaccggattg ccgcagttgg ggcaaagccg
     3301 tccctgcact tctgccggtg cagcctccgg agcaggagcc gctgtttcag gagccgtaac
     3361 cacttccggt tctgcgggct gtgccgccgg tgcttctgcg ggagcagctg ccggtgcggg
     3421 agatacggga gccgtttcag tcggaaccgg ctggggaatc ggctgttctg ccggagcggg
     3481 agccggagcc ggtgcaggcg taggtgccgc agccacaaag ggatttccgc agaaggtgca
     3541 gaacatcgca ccagccatgc ggggcttgcc gcagttcgga cagcactctg caccagccgg
     3601 agcctgtggt gcctgagcag ccgcctgacg ggcagcagct tccgcttccc gctgtgctct
     3661 ttcggcggct tctcttgcgg cacgttcttc tgcaagctgt ttgcccaccg gattttctgc
     3721 cccgcacttg gtgcagtaca gcacagtttc ggggatctct gccccgcagc cctgacagat
     3781 cagcaaatgc ttggtcttac ggatctgttt ccgatattct gcgatctttt cctgacttgc
     3841 cgcaatggca gacagaaatt cctgaaattc cggttccgcg tctgcggtat gcagttcagc
     3901 gtacttcctg cccatattga gataggcggc gttgatgttc ttttcttcat cgttgatcag
     3961 accattcaga cgaatggttt cggtagaaga cttggttttt tctacaacgg aatgtgccgt
     4021 attggtgaca gtttttccaa gtttatcgaa aaaagacata tggattcctc tctttctttt
     4081 aatgttacag gttcttttaa tgttataggc aacagtgttt ccggcgattc tacaataaca
     4141 tagaacaacc tatgatacat tatactatat ttttcagtat catgcaagca tttctctgca
     4201 attttcacaa cattttcatg ttgcggcaag ctgcaccccc atttcaggtg tgaaaagggc
     4261 agtgttccgg cggcggattt ttgccggcaa aaattgcgga aacactgcat aaagccgtca
     4321 ggcagaattt aggaaacact gaataaagcc cgcctgacgg
//
LOCUS       DAWBGR010000190         4337 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4789_length_4337_cov_3.355441, whole genome shotgun sequence.
ACCESSION   DAWBGR010000190 DAWBGR010000000
VERSION     DAWBGR010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4789_length_4337_cov_3.355441"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1213..1222
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1906..1915
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1086 a         1025 c          964 g         1242 t
ORIGIN      
        1 ccgacaaagc ggtcagaatg gaacgatggg aagtgcctgc ctgtgtggac ttgatgcccg
       61 catttgccat taagccgatt gcctgtgcgg tatcttctac agagaacccc agcgcacccg
      121 caacaggtgc ggcatacttg aaagtttcgc ccatcatgga cacattggta ttggcattgg
      181 agcttgcagc cgccaacaca tcagcaaaat gaccgctgtc ggcagcagtt aagccgaaag
      241 cggtcaatgc atctgtaaca atatcggatg tcgtcgccaa atcctcaccg gatgcagcgg
      301 caagattcat aatgccctca ataccggaca gcatatcttc tgttttccag cctgccatcg
      361 ccatgtagtt catggcttca gcggcttcac ttgcggaaaa ctttgttttg ctgcccatgt
      421 ctcgtgcttt gtctcgcagt gcatccaatt cttcaccggt tgcaccggaa acagcggcga
      481 ccttactcat ggcagaatcg aaatcggcac ctgtctttac tgcaagcgtt cccaaggcag
      541 tcactccggc tgttatgggc atcagttttt cgcctgcacc ggagattttg tttccgacat
      601 tctgcattac ttcaccggca gcaccaattt tcgcaaatgc agtatttgtt ttatctgcct
      661 cggtttgtaa acggcggagt tcctgttctg tttcgacaat ttcacgctga agtgcatcat
      721 attgctgctg cgaaatctca ccgtttgcaa gagcagtgtt tgcctgttct gcagcaatct
      781 tcagggtaga cagtttgtct tttgtggcgg aaaccgcatc ggcaaggagc ttatgctttt
      841 gggagagcag ttctgtattg gtcgggtcca gcttcagcag cttttccaca tctttcagct
      901 gtgtctgtgt atttttgatg ttcttgttga cgctctccag agccttggaa agcttggtgg
      961 tatcgccgcc gatttctacg gtgatgcctt tgatgcggtt tgccatgggg gtcacctgcc
     1021 ttttttcaaa aataggttga atttatccta acaatatggt ataataacag caaggaggtg
     1081 ttcgtatgat gatagataca aacacaatta tttctatgac agaagcaaat cagaattttt
     1141 ccatggtaac aagaattgta gaccggtatg gaacggccgt tattttcaaa aataataagc
     1201 cccgttatga agnnnnnnnn nncggatgaa gaagtgcttt ctgtttcaaa aaaactgatg
     1261 aaacgcaacg ctgctgttta tgaggaactt gccaaatgaa acgactcaca aaggaacagg
     1321 taatgctgct tcacaaagaa ttggtgaagg aatcaggcgg ctcagcggaa attcgtgatg
     1381 aaggacttct ggattcggca ttgaatgcac ctttccagac gtttgatgat gcagaattat
     1441 atccgacaat catagaaaaa gcagctcgtc ttggatacag tttgataaaa aatcatgcgt
     1501 ttgtagatgg aaataaaaga atcggcacgc atacaatgct tgtatttctt tctctgaatc
     1561 atattgaagt ggaatatgat gacgatgaat tgattcaaat gattctcgga attgcagccg
     1621 gtgaaatgga tgaccgacaa ttgcaggaat ggctgtggaa acacatcata taggttaaaa
     1681 cgcatcaaaa tcctcctgcg ttgccagaga atcatagtta caatcatcat tctcacgttc
     1741 tgtaaacata tcattcacca aaccaatcgt cagcaaatcc agatcgccca ttgacagtcc
     1801 taattgtaca caccgcagca gaaaaagcgg cgttgtcatt tggcggtcag tcgggcgatg
     1861 ttttttttag attctgcctg cgtttcgatg ttcagacccc acaagnnnnn nnnnnccaca
     1921 actcaatgag ctgaggcagg atttcataaa tgctgaatgt gttgaagcct tccagccact
     1981 cgtcgggaga atcgggaaca tttgcagggt cagcgtgctt tgccataatg tatgcgatgt
     2041 tttcaaagac ttccagactc tcaatatcga gagcagagtt ttcggaatca ttttctccca
     2101 cagatttttg caggctggag aaatctttgt aaatatctct gtgaaatttc agacgataaa
     2161 ggcgaggaac cgcagcactc gccttaaacg gcacttccat gccgtccacc agaatatttt
     2221 tcttgattgc catagcgatg cctccttatg aacttgcctt tgtggttgtg gttttcgctg
     2281 tcgtatcggg attgtacggc atcttgtacc agttattgta ggtgctttca tcagttgctt
     2341 cacaggtttt tgccttgacc aatccggtgg gaagtgccga tgcggtcagt gtcagtgttt
     2401 ccgtcttgac ttctgtggaa tcttccttgg tctgaccttc tgtggccggt ctgcttgccg
     2461 tgcagcaata cagtacatgg cggatcttgt gcttatctcc cgaaaattca aacatcaatg
     2521 caaactgcga cggttctgcg gtgttcttct ccacaagcac accgttgtta tcgagaattt
     2581 ctcccagaat ttcggtggca aattcggtgg tgacaagcgc cacttccagg tcaccttcgt
     2641 aaccggaatt gttgttgatg acatagtaca cactgtcatc ggcataaaaa ttctcatttt
     2701 caccattggc atcaatggac agtgaaaccg cacccggtaa acgcatggat tcaccgtaca
     2761 caggcacggt gggtgttccg tcagcgtccg caccccactg ggtaatcttt gcccaatgga
     2821 cgttgctcag accaaacttg accttgtttc ttttgttcgc cataaaatca aacctccatt
     2881 tcataaagca cttcatagag cctttccgac tctatccatg cttctgtctt gttgtaaaaa
     2941 atatgatgct gccttaaaat ctcctccaca cgttcttcca cttccggtga tttcttatcc
     3001 gtgtacagtt caatgtccag ctgtttgaag ctgtaataca tcagattatc agccgaaaag
     3061 gtatgctcgc cgggagaaag gaaaagtaga aagggcggtg caggactttc gccctctgca
     3121 aagtgatgat aggaaaaagg acaatccatt tcctgcatca tttcattgat ttcttcatag
     3181 gtcatgataa tgcctttctg atcagatttt ccaataattc ttcgccattc tgttcagccg
     3241 gagcaatatg cggttttcct gcaacgcgcc caccgccacg cttggcgtga ccatgctcca
     3301 ataaatgagc cagctgatag cggttcttgg aataaaccgt catttgcaga gaatggctgt
     3361 tctcgctgac ttttttggct gtccagcttt ttgcgtaagc accagtgtct tccggagcat
     3421 ttgaagatat ctcttttcta acttcggttg cagtttttcg gactgccttt ttcacctctg
     3481 tatcggcaag gtcagcatat tcctgtaagc ctttcatgat ctcacttgcc atgtcatcaa
     3541 tagatgtcac tgggagcacc tgcctttcgg acttctccct caattgcgag atagtccatt
     3601 ttttcatagt cgggttttac actgacaata tcaaatgtct gtccacggaa cagaatcctg
     3661 tgtgttgtgg cgttcaaaga cagcagatag gaactctgcc ggacaaggaa tgacacggac
     3721 tgtatttctc tggtgactcc cgtattcact tgttcggcag agcttttcac gctgactttc
     3781 gcccagcagg agaaaacctc gtcccacttg gaagtatggt ttccgatttc atctaccacg
     3841 gtgcgatgct ccagaatggc gatacgctga ttcagtttat caaaatccat tacaccacac
     3901 cctctcgctg tgcaaacaga atggaacgca ggctcatggt aagaccatga tagtcgggct
     3961 tgcttctgtt ttcatacaga taaccgagtg caaacaatac cgctgttctc gtcacatctt
     4021 caaaacaagt gaatttttcc tcatccattc tgcccacgtc cttgaccagt gattttgccg
     4081 tatcgagcag ttggaggatg agcttgtcat cctcctcatg gtcgacacga agataatttt
     4141 tggcttcgtt aagggtaatc atgctatcac gcctttttga ttgtaagagt ttttacggcc
     4201 tcgggcagaa tcagcttgcc gtccacacgc tgtgatgcaa gaaaaccgac ctgtccgttc
     4261 atagcgaaaa gctcattcag acgcttaagg aaacgctgaa caaaagcaca gttgaaaaag
     4321 caaatcgata attacga
//
LOCUS       DAWBGR010000191         4290 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4855_length_4290_cov_10.735537, whole genome shotgun sequence.
ACCESSION   DAWBGR010000191 DAWBGR010000000
VERSION     DAWBGR010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4855_length_4290_cov_10.735537"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          992 a         1116 c         1024 g         1158 t
ORIGIN      
        1 aagccgtcag gcgggctttg tgcggtattg cctatactgt attatcgcat acgtttatgg
       61 aaactgcgtg aaaatcctgc gtgaagctgc aaaaagctgc gtggaagaaa cttccgcatt
      121 tcaacgggca acagaaaaac gcccgacaga atctgccgga cgctttcccg aaaaataata
      181 aggagaatgt atgaaaaaac aagatgtcaa gaatgttgga gcaacgccgc acaaagcctc
      241 tgcatcagat gctttgtttg atgctgcatc aagaacgcag atcagttatt ttctgtctgc
      301 tgcacttctg ccagtgtcac gtcaaccttt ttggtttcgc cggcacggac ataagtcagc
      361 gtgatcgtat ccccagcctt atactggttc ttgactgcgt tcagttcttc tgtggtgctg
      421 atcgtctgat cctgaatgcc ggtgataaca tcacccggct gtaaaccagc ctgatcggct
      481 gcaccgccgg cggtcacgct aaagatatag gcacccaccg gaatattgta agcctgtgaa
      541 accgcttctg tcacgtcctg acaggagatg cccagctgcg ggcggccggt cacatagcca
      601 tagttaatca aatcatcgac aatgctcttt gcctccgaca tcggaattgc aaagccaatg
      661 ccctctactg tcgcttcacc ggaataggtg ctggacattt tggcggagtt gataccgatc
      721 acctgaccag agctgttgat cagcggaccg ccggagttac cgttgttgat ggcggtatcc
      781 gtctggatca gcgtcatggt gttgtcgtta atggtaacct tccgatccag tgcggagata
      841 atgccgcagg ttacggtgtt ctggaactgc aagcccagcg ggttaccgat tgccacaacc
      901 atttcgccga cttcacagct gctgctgtcg ccgaattctg cgggagtcag gttgtctgca
      961 tcgattttca atacagcaat atccgcttcc ttgtcatatg ccacgattct ggcatcataa
     1021 gtggtttcct cgtcagacat ctggatctgt acggaagaag cttcgccata accgtattcg
     1081 tcatcgtaaa taacgtgggc gttggtgaca atatagccgt ccttcgacat gatgatgccg
     1141 gtgccgacgc cagtcatacc ctgcgaaccg ttgttgccct gcgagccgta gccaaagaag
     1201 ccgccgtagc cgttcatggg gttattgttg ctgccgttgg acgaggagaa agtggactgt
     1261 acgccgacaa ccgacggtgt cactttcttt acaatatccg ctaccgacat ggaaccgttg
     1321 gtagaagcca gctgaatcca gctctgactg gagtcgctct ttttggaaga actgctgtcg
     1381 ccggtgctgc tttctgcggc agaactgtcg ttgcttgcaa cgatcgaact gctgttgtcc
     1441 gcatttgagg aacgaatgcc ctcatagaca ccgatcgaac cgccggacac cagaaccatt
     1501 gccagcacaa acgctgccac tttcagtccg gtatgctttt tcttcggctt tttgttgggg
     1561 tcgccgccgt tgggatcgga gtagtagttt acataagagt agccgccgtt gttgccgctg
     1621 ccgccgtttc cggtgggagt ggtctgaccg cctgccggat tgttataggc agagccggca
     1681 gcgttctgct gctggtatgc accgctgtgc actgcctcgt cctgatgcgg tgtggtgttg
     1741 cggtagtaac cgttctgata tgcatacgga tttgctgcac cgttgacacc ggaaccggca
     1801 tttcctgccg gagccgcggt atttgcagca ccgctgttgt tggtggtatt gtcggcattc
     1861 tgtgccacac cattctcatt ccattgtgca ttgtttttgt tttcgaagtc agccataata
     1921 ctcttccttt ctatcgtgaa ccgcttgttt tctgcggtat gtgttcttcg ctttctgtaa
     1981 ttattattat acggttttat gtgataatca tatgataatc gatagaaaag acggtaaaat
     2041 acagcccaca aaggcttcac attccctgaa aacagctttc acacaggaaa ttatgcctga
     2101 ttctgtgcaa gatagtctgc cagatcgaca tgaacgccgg caccggtctg tgccatatag
     2161 tacagccaca ggaaaatatc ctgcgaatcc agcgtgccgc tgttgtctgc gtccagagcc
     2221 tttcgctgaa tcggattcag ttcggtgtcc agaccagcac ctacccgtgc aatattgtac
     2281 aggcagagaa acagatcctg tgtatcaatg ctgccgctgg catcggcatc gcccatactg
     2341 catgccattt tctgatgaga tgcggctccg gtgatgatgc tgtctgccga gggacgattg
     2401 cttttcagat cgccgtcctt gccggtagcg gtataggcat tggaaatcac gctgccgtcg
     2461 ccgcaggaga gcatctccga ggactggatc gcagcagatg ccagcatgac ctgccccagc
     2521 ggagtgaaag tcatgttcag atccatgatg ttgctgaatt caaagccgat cgccttttca
     2581 ccggctgccc ctacataagg cgtatagaag tcggctgcag aagcattctg caagccgcgg
     2641 atcttgtcat tcagtccggc attgtctgca atggtcattg atgccagtcc cgtgccgctg
     2701 acattgccgc cctccaagga aacgtccaga tactgataca gatatgcgat tacagtcttt
     2761 cggttctggg atacaccgga catgccgtaa tagtccacgc ccagcggatt gttgacagtc
     2821 tgtactacca cattggcaga acggttcagg gacttgactt cagtaaccgc cgcttcaatg
     2881 tttgccgcaa cggtttccac cagagccggc agatcccgag caagcttgct gtttgctgca
     2941 gaaagcttcg agccatcact ggtcagcttg tttgccacgt cagtaagcgt cttacagttg
     3001 tccatatcca ccaggtcggc atttttatac agtacttctt ctataaagtc gttgacaccc
     3061 acagagatca ttaccacatc tgcggtctgc acatcgcgtt tcatattctg attggactgc
     3121 aagtcagaaa ccagcttacc ggaagtggtg cccactgtgg catagttcac cgcattgccg
     3181 cccagatagt ccgccatata ggaaactgcc gactcgctgc cgctgacttc tgccagacag
     3241 tcgtcaccca gtgccacgac cttcggggta actgcaaggc cggaagtgtc tgccgcaaat
     3301 accggcagtg ccagtccggt ggctgctgtg acaacggcac aggctgccgc aatgcctctt
     3361 ttccaaaaat gttttttact catatcgttt ttcctttctg tacaggcaga gccgccgtac
     3421 cccccttatg gcaacactgc ataaagctta cttgttgact tcatctgatg ctgccctatg
     3481 gcaataccac acaaagagcg tctggcgact ttgcacgcca tctgcttgca aatttctcgt
     3541 catatttcac aaaaatgctc gtgaatacat caagtattca ctccgctttt tgtttcatct
     3601 gacgaaaaat ttgacgacgc atctgtcgca caatctttgt gcggtattgc cctatatttt
     3661 ttcgcacggc tctgcatttc ctttacgata ttgtcattat accgtgtttt tccgattctg
     3721 tcaagataaa accgcacaaa gctgatgcat aagatgcatc accagatttt tcagcagagt
     3781 ttctccaaaa agcgaaaccg tgcctgccct caaaaggaca agcacggttt cagtaaagca
     3841 atgagaaaaa gagagataac tgcaaaattc aaaaattgca tgtggaaatg tgactgacat
     3901 ctcgcaattc tattttgtca gcccatatcc gttcgaagca tcatcacacg cgggatttcc
     3961 gttttctgtg aatcatctgc cttgcttctg tgataattat atcatatttg caccattctg
     4021 tcaacaggaa agtactgggt ttcgtgtaaa atatccaatt cgttttttgt gcaatttcac
     4081 aacacggatt ttatgcacca tattttcctt gtccagctgg tacaatttct gcaagcattt
     4141 cccttgtgaa aaacgttttc cacaatttta tccacttttc cacatacacc cgtgtggaaa
     4201 gcggtggaaa agggcgggct ttgtatggtg tttccttagg caataccgca caaagattgt
     4261 gcgacagatg cgtcgtcaaa tttttcgtca
//
LOCUS       DAWBGR010000192         4289 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4859_length_4289_cov_2.884270, whole genome shotgun sequence.
ACCESSION   DAWBGR010000192 DAWBGR010000000
VERSION     DAWBGR010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4859_length_4289_cov_2.884270"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1274..1283
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1014 a         1093 c          923 g         1249 t
ORIGIN      
        1 tctgggtttg ttccttccgg gtcagggctt tcaggatacg ttcgtcaatc gtacccttgg
       61 tgatgagatg ctgaatgaca accgtttccg attgctgccc ctgccgccac agtctggcgt
      121 tggtctgctg gtagagctcc agactccagg tcagtccaaa ccacaccaac acattgccgc
      181 ctgcctgcaa gttcaagcca tgaccagcag cggcaggatg tagcagacca acttgcagct
      241 ttccggcgtt ccagttccga atactgtcag aggactggat ttcctgatag gaaacattca
      301 gctttcgcag tcgctcttga atccgctcca aatcatgctt gaaccagtat gccaccagaa
      361 cgggtttgcc gttggcagct tctattaggt cttccagtgc atccagcttt cggctgtgaa
      421 tgggaatcac tgctccggtg tcgtcataca ccgctccatt cgccagttgg gaaagtttgt
      481 tgctgagggc ggcagcgttg gcagcagtga tttctatatc ctgcatctcc agaatcaatt
      541 cagacttgaa ccgcttgtag gtttcttgct ccatgtcgga cagctgcacg ggatattcgt
      601 tggaaagcaa ttccggcatg tgcaggtggt caactgcttt catcgaaacg gtgatgtccg
      661 atattttttc gtagatccgt tcttcggcat cgggcaggga tttgtaggaa tacacgatat
      721 agccgttctg cttatcgggc ttgaagtagg cgttccggta ctgcccgatg aatctgccga
      781 gccgctgccc catatccagc agacgaaatt ccgcccataa atccataaaa ccattactgg
      841 caggagtgcc tgtcaaacct acgattcttt tcacgttcgg tcgaactttc atcagtgcct
      901 tgaagcgttt gctttggtgg ttcttgaaac tggacagttc atcaatcaca accatgtcat
      961 agtcaaaggc ggtattgctg acaagccagt ccacattttc acgattgatg atgtagatgt
     1021 cggcatctgc cttcaaagct gcaatgcgtt cttctgccgt tccgactgca acgctgtatc
     1081 gtagcggttt taagtgctcc cacttttcga tttcagcaga ccaggtatcc cgtgcaactc
     1141 gcagcggtgc aataatcaga acctttctaa ccacaaacaa atcaaacatc aaattgtgga
     1201 ttgcggtcag tgttgtaatc gtcttaccaa gtcccatgtc cagaaaaagt gctgcaattg
     1261 gatgttcttc gatnnnnnnn nnntttcacc tccgagatga ttttttcgat gccctcacaa
     1321 gcatccaaga cataaaccag aaaacccaat cgcctcagaa gtttatgccg ggaaagttga
     1381 agcggtctgg gtttctctcc gggtgctttc acttccacaa aagcgatcct gccgccaggc
     1441 atcaatacaa tgcggtctgg aactcctgcc gttccgggag acgtgaattt ccagcacaca
     1501 ccgccatttt gctttactgc ctttttgagt ttttcttcaa tgattttttc tagcatggga
     1561 gtacatacac actttcacca tttttcatag cttcagttat ttcatcaaga acccttggga
     1621 catcatctat tttggcaaga gttctttttt tcctatcggt cataatcaca agattttcag
     1681 gatgtaatgt gtcctgatag agtgcatcaa atttttcaag gttaaccaaa agattatttt
     1741 tcttgatcca cataatgttt cctcctaaaa agtacgcaat atgggaattg tgccgctcgt
     1801 agccggtcat ttacaagcct tatatataga agaaattttt actttttttc tcgcctgcgt
     1861 aaagactgta tatgaccggc ttacaccggc acactcccga tttttggggc ttttttcgta
     1921 tttttgtgcc ggtcaagtta gtcgaggatg ccataagtca attgaattcc aatgatgtat
     1981 ttaccatccc tcatcttttt tctcttgtat cctgcctgtt ccagagccgc ataaaaatca
     2041 gaggtactgc ggatgtattc accattttca atgcagtatt ctctgtagtt gttgtaaagt
     2101 tcaccggatt tctcctgata gcttctattc acgatacagc attcattgat gaagtttcca
     2161 agccagtcat tgccttcccg ataggctccg attgcatcta aaacacactg cggtctgttg
     2221 atctgatagt ttgccgcaat cacctttctt gcaccctcaa tcagctagga aagcactgca
     2281 ccgcctgcgt tatccacaag atgctgcgtg tagtttttga tgtccttaga gccttgaatt
     2341 tttgcgtgaa acggaatgac aatcaatctc cgccatgtgc catcatccga tgcaccgacc
     2401 tttggaagat gattggtata cagcaccaaa gtatgagatg gttcaaagtg gaatggtgct
     2461 ttgaatttct tctcggcaaa aatcgggtcg gtcgagcaca gctgtttcac cacgctggtg
     2521 ttcagccgca tcccttcttg caattctgcc gcaataatca tccgttttcc tttcagttcc
     2581 gccatctccg gcttcacgtt tctcttgcaa ttgacggtca gtgcgtccgc tgaaatgtta
     2641 ccgctgtaac ttcccagaac cttgtaaatg acattccaga acgtactctt gccgtttcgt
     2701 ccgtcaccgt aggcaataat catcgcctcc aaatacacct tacccacaat acaaagtccg
     2761 caaatcatct gcacatagtc aatcaggctt tggtcaccgc agaagaacac ctgcaacgca
     2821 tcattccaga gttcttcgcc ttcattgctc ggaacgaccg ccgtcacttt cgttaagagg
     2881 tcggcagggt ctgtgggctt ccagccattc aatccttcgg gcagataata cgtgcctccg
     2941 ggggtattca agagcatggg attgctgtcg agggcttcgg gattgtggag cacaagcggc
     3001 tttgcggcat ccagtgcatt ggtcatactg cgaacatggc gatatttcat gacaaacgcc
     3061 ttgaaagcgg catagtactg atactccttg tatgcggcga tctgttcctc gtccagacta
     3121 tcccgaaact ttttaccgcc attaattgct gcatctcttg caacaccgag gctttccagt
     3181 ttcaaaagtg acgcttccac ctgcttttcc gcctctgcca gctgtacgtc tgtatgttcg
     3241 atcatagcaa gggtgacagc gtgttctgac tcctcccaat aggttccgtt gtagcgaaga
     3301 taatcggtcg caatggtaaa tgccacctca tctgaaaagc cttctacaaa tgtgcgagcc
     3361 tctccaacgt ccgaaaaatc atcgggaatc aaggactgtt tgccgtatgc ttcaggagaa
     3421 atatatcctt cctgcgaggt tacttttttt ccgaatttgc aggcactgtg ccagattgct
     3481 tccagttctt catccggtag cggcggttcg cattctgctg ctttttccag gaacttctga
     3541 taactttcct cagtcacacc aaaacgcttg accagttttc cagccatgcg agacattgtg
     3601 ctgtttcgct gtccctgcgg aatgttacga tttgacttca tcagtgtaag ccaatcctca
     3661 atggaaagac tgccttcatg ccatacaaca tcactcggac agccaaaaag aaaacgtgag
     3721 gcatccagtg cattgccgtc gaagaacggc agttccttat ggattttctg ctttatcgcc
     3781 ttatgagaat ttgcatcgtt gcagggtgct gtcggaaaaa atacatgaaa acgtggtctt
     3841 gcggatttat tccctttcgc cagcatatga tgacggctgt aggttactgc aaatgcaaca
     3901 tcactgagga aacttgccag tttttcctgt gtgatccagt ctttcgggtc atctgaatgg
     3961 tcgttgtcac aatccatggg tactacatca gacaacagaa aattggcatc actgcgggca
     4021 aaattctcat actgagcaca gacatgatcg tagacaacgg cttttttcaa atccgcttct
     4081 gaagtaatga ccttttggtt gggataaagg atattctttt cattgccggt acagtttgct
     4141 gtatagagcg taaatttcat tctatttcct ccagttcttc tgtaaaatgc cgaatggtca
     4201 tatgccgccg cttcgcccat ttgatttcct gctgcatacc ctccgaccgc acagaaccaa
     4261 acacccacag ctgggcacac tttgacagc
//
LOCUS       DAWBGR010000193         4268 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4889_length_4268_cov_8.472110, whole genome shotgun sequence.
ACCESSION   DAWBGR010000193 DAWBGR010000000
VERSION     DAWBGR010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4889_length_4268_cov_8.472110"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1225 a          955 c         1071 g         1017 t
ORIGIN      
        1 cgattgaaag aaatgaacgc tgaaattgaa caggcagatt atcagctttc tatgctgatg
       61 gcagaaacag aaacacagaa tttcacgcgt gccggaacga tgtttgctct gaccaccaaa
      121 attcgtgcct ctgctgtggc aggacgcaaa gaagaactgt atgcagcctt gaaagaaaac
      181 ggctgcggag atctggtcta tgaaactgtc aatgccaata gtctgtctgc ttttgtcaaa
      241 gagcagatcg cagaaaatca ggatactgta ccagagtggc tgagcggtct tgtccatatc
      301 tatgagcaga cctctgtatc tgttcgccaa tctgcaaaat gaaaggatga aatcaatgaa
      361 aaatgaaatg atggaaacca accaaacagg cttccttgct ctgcaggact ttgatcttgc
      421 caatgtgatg tgtgcagaaa tggacggctt atctgctaca tttgaacgag tcaagatccc
      481 atctggcggc ggtgtgatgt ttgaaattcc cggtgagaat ccggaggaac cggacacggt
      541 aaaaacattt tcagctgtga tcctctatca gcattctttg aatgcctact accaaagcga
      601 atatcagggc ggctccaatc cgccggactg cggcagtttt gacgggcatc atggggaagg
      661 aaatcccggc ggcagctgcg attcctgccc actgaatcag tatggatctg gaaaaaatgg
      721 tgcaaaagcc tgcaagaacc gccgtcgtct gtatctgctt cgggagggcg atatttttcc
      781 ggtaattctg tctctgccta ccggttctct gaagtccttt acccgttatc tgatgcgtgt
      841 gattcccaaa tacaagaatt ccaatgctgt ggtgacaaag ttcacactga aaaaagcagt
      901 cagcggcact ggcatgagct attcccaggc acagtttgct gtagaacggg cattgtctcc
      961 ggaagaatat ccgctgatcg cagctgtgac agaacaggtc aaggctctta gtaaaaatgt
     1021 gggctatgat acagaagatg cactgcgtgt ggacccggaa accggcgaag tcatggagcc
     1081 gctgaattga ggagaatgct atgaaaaatt acagatgtat cacttcggtg caggaaattc
     1141 agcagtatat cagcaatgcc gccgttgtgg cttttgacta tgaaactgca ccggatgagc
     1201 cttaccgcat cgaagaaaag gcggctctgg atccgcagaa aaaccatatg accggatgca
     1261 gtttttctgt caaagagtat accggaatat atgtcccagt ggctcacaaa gtaggagaaa
     1321 atatcgaact gacatctttt ttgcaatttc tgcgagcttt tctcacggac aagcgtatcg
     1381 ttaaagttgc tcacaatatc gcctttgaat ctgcaatatc ctgtcaacag aatatcgtca
     1441 tacagccgcc ggtgtatgat accatttgtg cagcacaaat gaccttgaaa agcaactacg
     1501 catttcgcaa acttgctgac agcggtctga aaaaactggc ggaagaattg tgtcatgaac
     1561 ggctgcccac tttttcggat gttacaaacg gcagacactt tgacgaactg ggtgcacagg
     1621 atatggaaac aatacgctat ggctgtgcgg actctgattt tgcactgcgg ctgtatcata
     1681 ttttcaacag ctggtttgac cgttttctgc cgaaacatcg gtatcttgtg gaacagatcg
     1741 aatcaccgac agcggtgtac ttagggctga tgaaatacaa cggcgtgcct gtgaatgcag
     1801 atttgatgaa agtgcgtcag caggagtcaa gacagcaaat ggagcgtatc cgcaatgaaa
     1861 ttgcaatgtg cattggcgat gttccgattg gtgcaaactg cagcactaaa gctttcaagg
     1921 agtatctgta tcagacttta cagctgcctg ttatgaaagt cacagcatcc aacaaagaag
     1981 cagcagatga tgcgtctatg atcttgctga aggagtggtg cgatgccaac cgtccggaac
     2041 tttctccttt gttcacactg gtacaggaat accggaaatg gagcaagatc aagtccacct
     2101 acattgacgg ctatctgaaa ttccgaaacg ctgcaacggg caggatccat ccggatttct
     2161 ttgcattgtc caccgaaaca ggccgcatga attgccgcaa tcccaattta cagaactgtc
     2221 ccagaaaaag caacgatccc atcggtgtcc gcaattttat tcaggctccg gaaaatcatc
     2281 tgatcttgtc tctcgatttt tcgcaaatcg aactgcgtgt gggagcattc tactgccggg
     2341 acaagacgat gatggaaacc tatcagaacg gcggggatat tcatgcggca accactgccg
     2401 tcatttttgg ctgtacctat gcagaagcac aaaacaagca tcgaccggaa tacaaggaac
     2461 agcgtaccat cgccaagaac gtgaactttg gcacatttta cgggctattt ccaaagggac
     2521 tgcagagcac attgaagttc aaagcaggcg tagagaaatc tataccggaa tgtgaagaga
     2581 tcattcgcaa tctgaaggct ggctatccgg ctttgacggt ctggcagaat gaaacaaaga
     2641 tgaccgcaaa gcagaaactg tatacagaaa cctggctggg acgcagaagg tatcttccca
     2701 atattcgcag cgacaactgg ggactgcagt cctttgcgga acgatgtgcc ttgaataccc
     2761 cgattcaggg aacggctgcg gatattctga agctggcaat tgtccgtatt ttagaaggac
     2821 tgccgtcacg cccatggctg agaccgatcc tgcagattca cgatgaactg acgtttctca
     2881 ttccgaaaga cagattgcag gaagcagtgg cttttgtgaa aggctgtatg gaacagcagc
     2941 cgtttccgga gcttgatctg ccccttgtgg cagaagcatc tgccggagaa agcttcggca
     3001 atttagagga actggaggaa tgaacttggc agaaacacac aacaaggaag gatacttctc
     3061 gccgaccgaa tttgaagcaa tgaaaagaat agagagcgag gagaaaaagg tacggagact
     3121 ggcggcattc cgaccgcttg tatatatctg ttctccctat cgtggcaata ccaatgaaaa
     3181 cattgaaaat gcccggaaat acagtcgttt tgcggtaata catcacagta ttcccattgc
     3241 accgcatttg ctttttccgc agtttctgga cgatacccta ggggaagacc ggcaaactgc
     3301 aatgttcatg aattatgtgc ttctgacaaa atgtgtccag ctgtgggtat tcggcagcag
     3361 catttctgag ggcatggcac aggagatcca atgggcaaaa cgcaggcata tgccgattcg
     3421 atatttcaca gaagagatgg aggaagttgt atgaacatat cggcacagga cgtgatcaat
     3481 gctatcttca acccggacga tacggtatgt ctgcgtattt ttgatgatcg gaaagaaggc
     3541 atctttaccg gtgccaagat gtccgtggag gcaggaaaat tttttgcagt agagtccacg
     3601 ctgaaagaac ataaccagaa aaatcatggc atcttttttg tggtgaactc cggcggtcag
     3661 accgatgaca gcatcacccg catcaatgca cagtttgtgg agatggacga taaaactttc
     3721 gaggaacagc aggcactcat cgatgcgttt ccgctgccgc cgtctatggt tatcagaaca
     3781 agaaaatccc tgcatacata ctggtttgtc aaagaggcaa aggtgtcatt gtttcgcccc
     3841 attcaaaagg cactggtgca gcatttcggc ggagatcctg cctgcgtcaa cgaaagccgt
     3901 gtcatgcgtc tgccgggatt ctatcactgc aagaaagaac cggtcttggt ggagtgcatc
     3961 tcgtttcacc cggaacgaag atacacacag gaacagctga tagaaagact gccggtttcg
     4021 caagaagcag aagaacaacc aaaagtaccg ctgcatggag aacagaaagg gatcggcatt
     4081 gtagaagcag aatgcgattt tatcaagtac tgccgggaca atgcagctgt actttctgaa
     4141 catgactggt atgcgatgat ctccaattta agtgtgtttg aaggcggtgc agcggtcata
     4201 catcgatact ccaagccgta tccgaagtat tcttttgaag aaacgcagaa taagatccag
     4261 cattttct
//
LOCUS       DAWBGR010000194         4248 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_4919_length_4248_cov_5.321727, whole genome shotgun sequence.
ACCESSION   DAWBGR010000194 DAWBGR010000000
VERSION     DAWBGR010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4919_length_4248_cov_5.321727"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1171 a          986 c         1105 g          986 t
ORIGIN      
        1 tctgcaagca gattgcgtgc aaagccgtca ggcgggcttt atgcagtgtt tccttaattc
       61 gcaatggaaa aggaggttac ttccatggac gataaaaatg taatggaaaa tatcctgctg
      121 ctggaaaagg gcgtttgcga cctgtatgta cacggcgtga tcgagtccag caccagcaac
      181 gtgcaccaga cgttcagtta tgcactgagc gattccctcg ccatgcagga taccctgtac
      241 gacaaaatgg cggcaaaggg ctggtatccc accgaccgtg ccgagatgac aaaggtacag
      301 gcggtcaaga agaagttcca gtcgatgcag ggctgacgca gacacaaaaa agctgccgcg
      361 tttttgcggc agcttttttc ggtgttgtcc ttgacatctg atacagggca tgatacaata
      421 caggcaacgc tgtacaaagc cggcaggctt tgcacggtgt ttccttaaaa tatcgtatgg
      481 tgtaaaatag agggtgtaat atgaaacgaa attttttatt ccatgtattg ctgctttctg
      541 gacttatcgc aattagcatc actggtttta caggctgttt ctatgaaaac acaaaagatg
      601 catcgcatgt ttctgaaacg gaattagagc agcttgcgga cgatgcagat gctgttgtcg
      661 aagcatattt taaagaaaag tatgatgtac aagctgctgt tacagaccgg tctattgccg
      721 ggggcgtttt tttaggaccg gatccgtcgg cagaacccta ttataattgc actgtcagca
      781 ttacagatga cgaggattat acggtgtgca atgccgaagt gtacggaaaa cgggcggaca
      841 aggaaataga attatacgta aaaaacgaat cctattatgg gaaattcatg aaagataaaa
      901 cgatacagtg gatagaacca tatatcctgc aagccggttt tcaggatcat atcatagagt
      961 acagttgtac ggagctatgc tttccgtcag aatattctgc cgatctcacg gcggatacat
     1021 ttgtttcatt cacttcaaaa gacagaagct tagccgcctg ttttcagttg atgatttcag
     1081 aatcagagta tatgcagcat gaggatctat ccagagaatt tgacggcttg aaagatcatt
     1141 tggagcaaat tgacggcgaa ataacattgc gtatgtatgt ctatgatgat gaagattata
     1201 agcagatcaa aaatggcagt gatgctcatt ttcatagtat tctatcctca gaaattttta
     1261 gctgcacagg gtgatacgac attgcataag atgtgcaatc cgattttgcg gtagcttttt
     1321 cgacgttgat tttgacaggc tgcacaaaaa atcttgacat ctcacgcaag atatgataca
     1381 ataaaaataa ttggacaatt ttttgcctgt aagagcagga gcgttttttg gaaaggaagg
     1441 attgattgtc tatgaagaag tggatcgcgg cgatttctgc cgcagtgctg gcagtgaccg
     1501 gaatggcttc ggcaattcct gcggcaacgg tgaccgctgc tgacagcaag tacaactatg
     1561 gagaggctct ccagaagtcg atgtttttct acgaggtgca gcagagcggc aaaaagccgg
     1621 attggaacga agtcagctgg cgttcggact gcatgaccaa cgactatgtg acaggcggct
     1681 ggtttgatgc cggagatcac ctgaaattca cactgaccaa tgcgtactcc gcagcactgc
     1741 tgggctgggg tctgctgaac tacggcgacg gtgtggaaaa ggctggtcag cgtaccatgt
     1801 atgagaacaa cctccagttt gcactggatt atctggtggg ctgcgatcag ggtgacaaca
     1861 ttgtctatat gatcggcgac ggctcgttcg accacgtatg gtggggctct gccgaagtat
     1921 acatggacaa gtacgaacta atgaagggcg aaactgagcg tccgtactat acctgtgagg
     1981 acagctgcat tcaggccgac atggcggcgg cactctgcac cggctatctg aacttcaagg
     2041 actccaagcc ggaaaaggca aaggaatatc tggaacacgc cattgacctg ttcgaccgtg
     2101 ccgacaagct gcgtgcgatc ggcgatgatg ccgcagaaca gccgtactac aagatcacca
     2161 cattctatga tgatctgttc tatgctgcaa actggctgta catggcaacc ggcgagcaga
     2221 agtatctgga tctgtgcaag accgactata tcccgaatct gggcaaggaa gaacagtcca
     2281 gcgaaatgaa gtacacatgg ggcatgtgct gggacgatgt gatgcagggc ggcgtgctgc
     2341 tgtatgcgat caacaccggc gaatcccagt ggaaagacca gttcaccaag catctggaat
     2401 actggacgac tggttacggc ggcaagcaga tcacctatac accggacggt ctgccgtggc
     2461 tgttccagtg gggttctctc cgccacgcta ccacgactgc atttctggca tatgtggcag
     2521 tcgatcagct gtatcaggac gataccgcca aggctgaaaa gtacaccaag tttgctgacg
     2581 atgtcatgaa ctactgcttc ggcgacaact ccaaaaactt tagctatgtg gtcggcatgg
     2641 gtgacgatta tccgcaggca tggcaccaca gaacttccag cggtgcatgg aatgacaagt
     2701 ggagcaacat cggacagacc gagggcgaag atgccaagcc ccacgcacac attctgtacg
     2761 gtgcactggt cggcggtccg gatcagaagg acagctattc tgacaagatc ggcgactatc
     2821 agtatactga agttgcgatc gactataacg ccggctatac cgcagcactc tgtgcaatgg
     2881 tagaaaagta cggcggcacc tctgaccctg acttcccgcc gacagaaact ccgaagtggg
     2941 acgaattctt catgaaggct tctgtgaacc agtcggcttc cagctatact gaactgaaag
     3001 cctttgccat gaaccacagt gcatggccgg caagaaccat caagaacctg tcctataact
     3061 attacttcga catcagcgaa ctggtcgatg caggttattc gatcaatgac gtttctgtaa
     3121 agatcggcta cgaccagcac agcagcgata agggcaagat ctctatttct gatccgatcc
     3181 agtacagcgg caatatctac tatgtcaagc tgtcctttgc agacggcagc gtggtaatgc
     3241 ctaccggtca gtcggaacac cgcagcgagt gccagttccg catttcgatt ccggacaaca
     3301 ttcagggtgt ctgggatccg acaaacgact attcctatgc cggtctggaa cagggcggcg
     3361 aagatgctat ggtggcaacc gaccatatca ccatgtatga cggcgacact ctgatctggg
     3421 gcgtagagcc ggacggcacc aagccggatc cggcagttac aacaacaaca acgacgacga
     3481 ctacagaaca gaccaccaca acgacaacaa gagctacaat gacgaccact tcgaacgaaa
     3541 ttatctatga atccgcgggt gcacttctgc tggacgatga gccggagaag ctgacctatc
     3601 gtgttgggga ggatctggat ctcaccggac tgcgaatttc cctgaagtac tatcacggaa
     3661 aagacagctg tgatgtgatc tatgacaagg tatcgcccgc cgattatccg gataaattca
     3721 ccatagacac ttcggaattt gacagttcca agtcgggcac atataccatt cgtgtgaaag
     3781 caagctcgga tttgatcttg aactaccggt tgtcctttgc cgaggtttct ttcaaggtta
     3841 ctgttgaaga tcatgagagt acaacagaaa cttcacctgt taccacaacc accaccacaa
     3901 ccgcttccgg acagcctaca ccgtcaggag cagtcctcta tggtgatacc aatctggacg
     3961 gcagagtgga catcaccgat gcggtgcttc tgaataagtc agttgccgga gccgtagttc
     4021 tggagggcga tgccaagcag aatgcagact gtgacggcag caacgagatc gattccaatg
     4081 atgcagtcgt actgctgcgg ttcttggtac acatcatcaa ttctctgccc agtgcagaat
     4141 gattccggtg atgtctgaac gacacgcgaa aaactgtata agggaaacac tgcataaagc
     4201 ttgtgcgtaa gatgcgtagt cagatttttc ggcagattgc ctagaaat
//
LOCUS       DAWBGR010000195         4195 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5002_length_4195_cov_2.847585, whole genome shotgun sequence.
ACCESSION   DAWBGR010000195 DAWBGR010000000
VERSION     DAWBGR010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5002_length_4195_cov_2.847585"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1088 a         1067 c          698 g         1342 t
ORIGIN      
        1 gccggattgc caagcagttt ttgcactttg tactctgcca actcgtgggc acacgtgcct
       61 tcacgagcat aatcagtgac tttatccggt aaaaccgcac aaagctgtgc ggagggtgga
      121 cacgccagcc aacgagcact ggatgaagca gaaagcactg cgtgtaaacg gtttgcatga
      181 tcgttaagtt ccaatctgct tcgcctcctc taacaagacc gcatattctt cgggagaaac
      241 accagacagc tttgatgccc cgtgtttctg aagcagtgcc tttactgaat ctgtaaaacc
      301 agaacgtgac ttttctgcca gtaccgctcg aatctcagaa atagaaacgg tcggcgtatc
      361 tttcacagac accggcttct gtacagcctc cgtattgcct tcttccggcg gatatacctg
      421 ctcaaatgtc tgtacttccc gttctgtcat ggtttccgcc atagcttcca atttgtctgc
      481 caattggcgg atcacatgaa tcacatccag taatgttgta ggttctttac tcattttctt
      541 tgaccttctt tcttagcatt tttgatggga tttagaaaca cgccatcatg caccacctcc
      601 ttccataaat gcagtcgaaa aaatcagcat aaaatcgaac cctatcagta gaaaaatcaa
      661 aattttttct tgatttgggc tttgattttc gccatgcgat gccgaattgc cgtttccgat
      721 acgccttctt ctcttgctac ctgtgtcata gggtttcctt ccacgaccac tctgcgatag
      781 gtatcctgct gcttcagcgt aagactggac acaacctcat gcaaacgctg gatttccaaa
      841 gattccactt cagtatcgac aggttttgca caatgttctt tcacctttcg ctgtttcaga
      901 ttacgataca cctcacggtc atccaacttg tgcaaaaagt cgatgatctc agtgcttaca
      961 ccctgttctc ccgaatgcag cacagcgact gttccatctg caaagcgata gatataaacg
     1021 gatctggctg ctgttcttgt tttacgaaat ttcatataca tgactccttt ctgattgata
     1081 gaagtcagct tgcaaaaaaa ctcaagtgaa gtcaagtata tgaaacaaaa atagccgaac
     1141 agcatataaa acagtcgtct catatactat ccggctattt ggtagtcaaa tcactccgtt
     1201 gctcggtata ttatctatct cttatcagcc atgcacatct cggatctgca ggaaactttc
     1261 acgatattcc ggcagtttgg gcatttcagt tcaataatca ctggaatttt aggtagcaca
     1321 gaaatatcaa aggcacgttt cccacatctc ggacacttca tcttatacac ctgctcacac
     1381 ctccaatatc agttcactgt atggcagtga ttctgcccac ttgcaaaatt catgccactc
     1441 atcaagttta tgattttttc ttgcttggca ggcatttcgc agcacttcat agttcaacac
     1501 tacagttctt cgctgattct aactttccgg aagcatctga atcatctgcc accagtaaat
     1561 cttctttttg gtttccaaat acttttctcg tgctttattg agggctttaa ttgtgtacat
     1621 aaaatctttg agaaactctg ttccttcctc ggtgccatta aacagatatt cgcatgaaaa
     1681 atcgttcaat acaaattcct tttcagcaat tttgtgcatt gtagagcagg aatcggtaac
     1741 cgttccgatt ttgtacgtgt caaattgttt ccaccaatag aaaggtgcga ttatatcaca
     1801 actcactgta atcattcgca taaatttccg atgatcagtt cctgctcgga ctaatttttg
     1861 cattaaggtc aaatcattat cgccaatgca aaacggattc ttgccaagat tagtccaagc
     1921 ccacccacaa tgagagcaac cagaattgtt acatttggct gttatgggtt ctttgcaata
     1981 acaactgtct gatttttccc acgaattttt cggatttcgc attccacgga tggctgcttc
     2041 ccagccgtat acttccgtat tttcgatttt tatcatgtca atccctccat aaatgccgcc
     2101 ataaccgcct ttgccactgc atccgctgtt tcatcaaatt gaatcaaaca ccgcttaaac
     2161 aattcggtct tgaaagatgc cattgtgcga tcatccattg cacctttttc ccgcagttcc
     2221 aaaagttgct cgttcgtaag cattgaccat agcagttcta atgtttcatc gctcatttcc
     2281 aattactcct tttcatattc taatttaatc agacacttta tcgctgctaa agctgtgtca
     2341 attgccgcaa catcaaggca aaaagcgtta tcttcttcgt cttcaaaatc agctgcaaag
     2401 ccctcacggt cgcaacgtaa gtcttccaac tgttcgattg cacttatcaa gttttcaatt
     2461 gactgctggt cttgggtttc aaagtcacct tcgtagccaa ctgaacttcc atcttcgcaa
     2521 attgcaaccg ctgttacatc atgcttaaca gcaactgcgg caacagtcat gtggatttct
     2581 tccgattcgg tgcatttggt tccgataaat gacattgata ccgcatctgc atatttgtca
     2641 tcaatctcaa caatcagctt tttcatgatt tccctcctgt tttatttcca ttagtttttc
     2701 catgtaccac tttcttcaga tttttgcagc gttttataaa atcagctctt tagcttaagc
     2761 acttcaattc tttttcgacc tcatacgaac ttttgtaagg gcgattttct cttgtccacc
     2821 tgtctgaatc ataataacaa gtttccagtc tatcttcata agcatagtgc ttgacataaa
     2881 tcaacgtcca gtgatttcca catatccact ggtcaacttc atatgtcaga taatcccaca
     2941 catattcgtc ttttcgggcg ttttcaacac ttccctgaaa aatttctaca cactggtcat
     3001 aagcatttct tgcataaatg aggacttgtc cgtagtatga ggtactagcc aacatctcat
     3061 gaagaatcat ctttctttta cctcctgatt taactccatc aattttccca tgtaccactc
     3121 cgccttttct atatcttcct gtccattttt ccgacttgcg cgaaaacggt atttatatac
     3181 gttgcacata cagaaatggc gaacagcatc cacgccaaac aatgcgacca tctcatcaat
     3241 gcactcgtac tttccttgat agtgaaatgg atgattcaca ttatccggac tcggatgaag
     3301 cccgatactt tccttacaca ttttctcatt caccaccttt cagttctttc tgacagaaac
     3361 cagaacagca tatcccttca tctgttatct gtattgtttt ctgccctgta ttctcgcaaa
     3421 caatgccacc ctgtttctgc gtaataaccg cagcaggtgt ccggatgact cttgtgtttt
     3481 tggactggtt tgcatatttg cagtttacac aatcgttcat tctgcctgtc cccattcaaa
     3541 aatttctcca gttggtttct cattgcccca ccgcaatttt ccatctcttg ttgcaaacca
     3601 gatattttct ttcggaatca ttccgaaaat cccatacaac gctttttcaa tctcacttgc
     3661 attgttaaag tcacgaaata cattcaactt tgtcggacga tctccggttc gatctgtcaa
     3721 atgatgctct tcgcaagcct gtaaaaaggc atcagtgttg gaactgttcg tctgtaccca
     3781 tacgtcaccg gaaataaatc tgtcccaatc aaaagccgtt tccggtgcag aacccataca
     3841 atcaagcagc cgctccaaag ccaattttgc accaaaggca aaatcaaaag catcctccgg
     3901 acagcacctt gcaatgcttg cgtttacttt cttgccgtta acatactgtg tagccatcac
     3961 tgcgttccca ttttgcaaaa tgacaacctt tgtttctttt tcaatcttca ttatttttgc
     4021 tcctttcatt gaacggttga ggcagtgaca tccaagccag cacctcataa ttttcgtctt
     4081 catcagttat ttcaagaatc tttgagtaat cccaaaactg ccagtagtta ttgccacgct
     4141 gcccatagta tgtattacta aaatccgtgc atctgtttcg gaccgttatc aatac
//
LOCUS       DAWBGR010000196         4191 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5007_length_4191_cov_3.731867, whole genome shotgun sequence.
ACCESSION   DAWBGR010000196 DAWBGR010000000
VERSION     DAWBGR010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5007_length_4191_cov_3.731867"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1170 a         1039 c         1215 g          767 t
ORIGIN      
        1 caagcagatg gcgtgcaaag tcgccagacg ctctttgtgc ggtattgccc taaatcacag
       61 aaaggggaag ctttcatgaa aatcttacat cttagcgatc tgcatctggg gcggcgaatg
      121 tacaactatt cgttcctgcc ccaacagaaa aaactgctgg agcaggtggt gaccattgcc
      181 gaagaacagg cggtgcaggc ggttctggtg gcgggggata tttacgataa aaacgtaccc
      241 gttgcagaag ctgtcaccgt gctggacgag ttcctcacag cactgtcggt gcggcagata
      301 ccggtatttc tcatcagcgg caaccacgat tccgcagagc gtctgcaatt cggcagccag
      361 ctgctggaac accagcaggt ctacattgcc ggaaccttta ccggaaagat gcagacctac
      421 gatctggaag atgcatacgg aaaattacac ctgtgtctgc tgccctttgt gaagcagtcc
      481 acgctggctt ctctgtatcc ggacgaaaaa ttccacagcc tgtcagaggg catcgcccat
      541 gttctcgagc agacaccgct gtcagaaaat gaccggaatc tgctgatgta ccatggcttc
      601 gtgctgcaca acggcgacgg gccggaactg tcagaatcag aattgcagct gggcggcaca
      661 cagctggtgg aagcgtcggt attttcggca tttgactacg tggcactggg gcatatccat
      721 aagccccaat gggtaaaaag cggtaagatt cgctacagcg gttctctgat gaagtattcg
      781 ttttcggaaa gcctgcaaaa caagagtgtc acactgctgg actggaaagc gaaagatgac
      841 ctgaccgtca ccacgatccc cctgcagccg gaacaggata tgcgtgtcat tcagggaaaa
      901 ctggaagacc tgctggagca tgcagagcct tcggacgact ggattcgtgc ggaactgacc
      961 gacagggaac tgatccccta tgctctggaa agactgcgaa tgtcttatcc caatcttatg
     1021 gagctggtgt atctgtatca ggaggagcag attgccgcca gcaatacgga agtgaaactg
     1081 gtgccggaac agtcgctggt ggaactgctg ggcggctttc tggacagcgt atatcagtat
     1141 cagctggagg aagaaccgga ggcgtatcag ctgatggaag aaatctgtaa ggaggcggag
     1201 caggaaaaat gaaaccattg caattatcga tgaaagcatt cggggcattt gcagaggaat
     1261 gccgcattga ctttacgccc tttgacaagt cggggatcta tctgatcacc ggcgaaaccg
     1321 gtgcgggaaa aactaccatt tttgatgcga tctgctacgc cctttatgga acggcaagcg
     1381 gcgatgtccg cgatccgaag cagtttcgca gtcagtatgc tgaccccaag cagatcactt
     1441 gtgtagaact gcggttcttc tgcgggaaca aacagtatct ggtaaatcgc acaccgccgt
     1501 atctgcgtgc ggcactccgc ggcagcggca tggtagaaca aaaggcagag gcagaactct
     1561 gggagattct tccgaataag acggcagaga aaaaagagat cctctgttcc ggagcacagg
     1621 cagtgacgga caaagtggtg gaactgaccg gcatggacag caaacagttc cgccagatcg
     1681 tcatgatcgc acagggcgaa ttccggaagt ttctgctgtc cagttccgtg gaaaagggaa
     1741 agatcctgcg gcagctgttc cacacccaga actgtgacaa gattcagcag atcttgaaag
     1801 agcggctgga tcagcagagg gcacatgtca gggaacagga aacccagatg ctgaccctgc
     1861 tgcatcaggc ttcaccggca ggggaaaaac aggcagaact ctatgaagca cggctgaatt
     1921 tttccggcat ggcggcagca gaggaactct gtgacatgct gaaaacatca gcggcggaac
     1981 tgtcggaaca ggcagcatca cagtccgaac aggtgcagga acagcagaaa gccatagaat
     2041 ccctgctaca ggaaatcgaa cagggcaggc agcacaacgc cgttttgaaa cagccgcggc
     2101 aggcggaaca ggagcagcag aaaggtgctg tccagctgga actttgcgaa gctgcggcga
     2161 aaaaagccga tgccgatgca gaaaaggtac cagaattgca ggaacaggtg accctgctgc
     2221 aacacagcct gcccaagtac gaggaagttg ctgtgttgca ggcggcagaa tctgctttac
     2281 agatgaagcg gaaaacacag gaacaggctt gtgcacaggc gaagctgtac tgggagcaga
     2341 ccaacgccag ctgtcagcgg ctttccaagg aattggaagt ccacgtggat tctgcggtgg
     2401 actgtgccaa tctggaacat gccatcgaaa aggacaccca gcggcagcag agcgtccggc
     2461 agctgcaaaa gcagatgacg gaacagcaga aagcacagaa agaactggaa cgccggagcc
     2521 agcgtgccca gcaggcggaa acatggtatt tcagcgagga aaagccagca tacgaccgga
     2581 cagaacggca gtttttccag agcatggcgg gcaatctggc acggaagctg aaggagaatg
     2641 agccctgtcc ggtgtgcggt tcacgggaac actcgtgtcc ggctctgtac acggaggaca
     2701 ccgttacaga agaacaattc caaaaggcaa aagaacggca gcagaacgcc ttacagcaaa
     2761 tggaagagca taagaaatct ttcacccagc aggaggaacg ctgtaagatg ctggaacagc
     2821 aggtgcagca gcagctggtg cactgcggac tgaacgaaac cgccggctcc gcagcggttc
     2881 agcagtttga ggcagatttg caggcagcga tcacccagaa caaccggctt ttgaaagaac
     2941 ggaaagagca gctgttggca cagcagcaga agaagcagca gctggcaaag cagcaggcag
     3001 aattgccccg gttacaggcg gcttatgaga gcagccacgc ccagctggaa cggctgacac
     3061 aggaactggc agtgaaaacc gcagaacggg aacagtgtca ggcgggactg ccctatgcat
     3121 cgctgtcgct ggcaaagaaa cagctggagc agaagcagag caccatttgg cagctggaac
     3181 aggcacagca gcaggcagca cagaaatggc agcagcagaa agaacaggtt tcttcgcagc
     3241 agaaactggt gcagcacctg cgggaaacag tcaaggacaa gccggcgtat gatctgcaaa
     3301 ccgcagaaca gacgctggaa cagaaacgca gcgaaaaaga acgcactgaa aaacgctgcc
     3361 ggcagtccag tcagcaggca cagcagtgca agaccattgc cggagaggta cagaaacggt
     3421 atcgctcttg ggaaagcgaa gccagacagg ggaatacctt ggaaaagctg taccagatga
     3481 tgactggtac gctgaaaaaa ggcgaacgca tttcgtttga acgctatgtg cagacttatt
     3541 atttcaatcg ggtgctgcag ttcgccaacc agaagctcta tcagctttcc aacggcagat
     3601 accagttccg gcggcggcag gaagaaacgg tacacaacat ttcttcgggg ctgaatctgg
     3661 acgtgctgga tcagtatacc ggcaagtcca gaagtgtgga aacgctttcc ggcggcgaaa
     3721 cgtttctggc atcgctggca ctggcactgg gactttctga caccgtgcag cagcaaagcg
     3781 gcggcattca gctgaacgcc atgttcattg acgagggctt cggctccctg gacagcactg
     3841 cactggacaa cgcggtgcag gtattgcagc agctgtcaga ccaccagcgg acgatcggca
     3901 tcatttccca cgtgtcggaa ctggcagaac ggttcgaggc acagcttcag gtggaaaaaa
     3961 gcgaaaacgg cagccatgtg atctgccatt atgaataaag gcaataccgt acaacgccct
     4021 ttgcagacga gttcctctgt ccgcagaggg cgttttaccg tttttgcaca attttttgac
     4081 aaaaatgttc tgcaaaattg tttgtggcat tttcatttcc tgaaaatgcc gccttgaggc
     4141 aataccgcac aaagagcgtc tggcgacttt gtacgccatc tgcttgcaaa t
//
LOCUS       DAWBGR010000197         4165 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5042_length_4165_cov_4.908759, whole genome shotgun sequence.
ACCESSION   DAWBGR010000197 DAWBGR010000000
VERSION     DAWBGR010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5042_length_4165_cov_4.908759"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1031 a         1033 c         1112 g          989 t
ORIGIN      
        1 gagtaacaca tacgaatcac taaaggatgt tgttgagtcc gaaaataagg aaaatgctat
       61 tgcattattg aaatcatatc ttgataatca atggtatgat atccataaag aatgtgcatg
      121 gcatgattct cataagtcaa caaaaacaac gtattatgga tattggagtt ttgaatcagg
      181 ggcattggct aaagcacttg aactcgatga ttcatcatta aaggaacaac tctattatcc
      241 gtatgactta gtacataggg gtgatgacta atatggataa taacgagcag tttaaagtcg
      301 aatcctatat ttctgattcg cgtcttgcgt ttttggtgtt tcactttctg gtgaaaatca
      361 tcagcggcaa tacattagag aattgggata tagaaataga aagaaataga aaagccgccg
      421 ctgtgcaatt tttcatgcac ggcggaggct ttttatcgga atgtgatagc aggtttcggt
      481 gttgcttttc gtatactcaa tgcagggcat gcggtgccag ttctggcacg ccaatagaag
      541 ttgttccctc ccgcaggagc accagtgtca gttctgccat gtgggtcggt gtttccggac
      601 atccgttttt cagccagtgt tcgatcagtc ccatgcagcc ggcgagggcg aaggaaatgg
      661 catattgcag atcctgttcc cgcagttctc tgggggcaaa tgaccgcagc gtctgtacgc
      721 ccttttcccg aatcatcttt tcaatgttcc gtaaaaaggc aatgtcgccg ttcggtccga
      781 tcagggcaga acaaagctgt gcattttccg agagaatggt gaaagcctgt tccagaaacg
      841 cgaaaaagtt tttcggcgtt tccggcaagg tccagttctg gtttaccgcg tccagcagct
      901 gctgcaacag ccgctgctct gtcttttcca gcaggtcgta aatgtcggta tagtggagat
      961 aaaaggtaga gcggttgatg tccaccagat ctgccagttc ccgcactgtg atttccttga
     1021 tgcttttctg ctggagcagg gtagtcagtc cgttcaggat cagagcctct gtgcggcggt
     1081 accgccgttc ctgtttgata ttatttgtca tgatacatct tcctttatct agcatagatg
     1141 ctttttatcg ttttccttgt catgacattc ggggctgtca taacacataa tagacacgat
     1201 gtctattact aggcgaaatt tgcaaaatca acggttgcaa attatcttcc cttatattat
     1261 aatagataca ggacgaaaag tcaacagttt tctatttgta gacaaaaact tttttgtaga
     1321 cagctgtgtt tcgtcactcg cagagaagca aaactgtgca ttcggggaaa gcagtctttg
     1381 tttcacaata gaaaggaagt aggtatttat gcgatcgagc gattccggaa aaaagcagaa
     1441 ctttttctgg cggcatttca atggtatcgc tatggcggca attgtgctga tcccgaccat
     1501 ttacaccacg ctgtttctgg gctccatgtg ggatccgtac ggcaacgtgg accagctgcc
     1561 ggtggcagtc gtcaacaacg acaaatcggt agactatgag ggaaagaccc ttgccgtcgg
     1621 agatgaactg gtggataagc tgaaagaaaa tgaccagctg gatttccact tcgtcagtgc
     1681 agacaaggcg gaaaagggca ttgcagacgg cacatattat atggtgatta ccattccgtc
     1741 agatttctct gaaaatgctt ccaccctcat ggacgatgag ccgaagaaaa tggaactgca
     1801 atatgccacc aatcccggca ccaactacat cgccagcaag atgagcgaaa cggctctggc
     1861 aaagatccag aaggaagttt ccgaaagtgt gaccaaggag tacaccgaaa cgatcttcga
     1921 tcagatggga accgtaggcg acggcatgag tgatgcggca gatggtgcac tgcaaatcaa
     1981 ggacggtgtt tccgatctac aggacggcaa caccaccatt accgacaatc tgaagctttt
     2041 gtctgacagt acgctgacat ttgtagacgg tgcaaagacc ctgcaaagcg gtctgcaaac
     2101 ctacaccgac ggtgtggcaa ctgtcaacag cggggctcag actttgtctg gcggcatgga
     2161 tcagctggca agcggtgccg gcacactggc agacggtgca gtggcattgt ctgacggttc
     2221 cggcacgttg tccagcggtg tcaacagcta tacggacggc gtggctgccg cttacagcgg
     2281 tgctcagacc ttgtccggca acagcagcca gctgcaaaac ggtgcagctt ctctgaccag
     2341 cggtgctgca cagctgcaag ccggcagtgc acagatgacc gctggtttgc agactttgtc
     2401 tgacagtctg ggcagcagcc tttctgcgga gaacctccag cagatggaac agctgaatgc
     2461 cggactgaca cagctgcagc agggcattga caccctgaac accacgctgc aaaacacttc
     2521 gatgccggat accagtgcac tgacacaggc actgaccgca agcctgactg cgatcggcac
     2581 cagtgctcag gatgccggtg cacagctgca aagcttgcag tcggcattga ccacaatgac
     2641 ccagacagag gtgttccagt ctttggatgc cgcttctcag cagcagcttc tgagctgctt
     2701 ctcagagccg atgaccgcac tggcaaccga tgtttcgaac atcggcacac aggtgaccgg
     2761 cttgtcccag acattgcagg gtacagatct cagcagttct gccgccgcaa tggatcagct
     2821 gaaaaccagc gtcagtgcat tggcaactgg tgcagatcag gcactgccgg gagcacagca
     2881 ggcgatttcc accttgtccg gcggcttgca gtctgtacag tccgcagtga atgcccagct
     2941 gatccccggc tctcagactc tggaagctgg tctggatcag ttggcaaacg gcagcagtac
     3001 actggaagcc ggcgtatctc agtataccgg cggcgtatcc agtctgacag acggcttgca
     3061 gacactggac tccaacgccg gtgcactggt aggcggtgct tcttcgctgg atgacggtgc
     3121 caagacgctg gcaaacagcg tgccgacact gaccgatgcg gtttcacagc tgcaaaacgg
     3181 tgcggctcag ctgtccgacg gtacagcaga actggcagcc aataatgcga cactggtagg
     3241 cggtgcttcg cagctgacgg acggtgccgg acagattcag gacggtgctt ccaagctggc
     3301 agacggttct gtggaactgg gtgacggtct ggtgcagctg tccaacggtg ctgtgacact
     3361 ccagacctct ctggcggacg gtgcggacga agtcaacagc atcgacacca ccgacaagac
     3421 ctatgacatg ttctcggctc cggtgatgac caacgaaacc tatgaaaccg ccgttcccac
     3481 aaacggcaat gctatggcag catatatgat gtccgttggt ctgtgggtat ccggtctggc
     3541 attctgcgtt atgctgtcgc cgtatcatca gaagatcggt aaccgcaagg caggaaaggc
     3601 atggcttctc acgctggcaa agctgtgggc aatgggactg ctgcaggcag tactgatgat
     3661 cctgtgcctg tcgtggatca acggcttcca gccggaatac atgggcaaga cactgatcat
     3721 tgcatgcctt gcttctatcg cattcctgac actggaatac tgcgtcaact tcttcctcga
     3781 cattatcggc gactttatcc tgctggtatt catggtgctc cagctgagcg gctgtgcagg
     3841 tacctatccg ctggaactgt ccgataagtt ctatcaggta ctgaatccgt tcatgccgtt
     3901 cacctatacc gttcacggct tccgcagcgg tatcgcgtcc ggtctggaca tcaccaccga
     3961 ctgtgtggtt ctggcagtga tcgctgtggt attcgcagga ctgctgctgg cgggcttccg
     4021 taagcgtgcc aagaaggaac aggaatctga agtggaagtt gctgaaaaag caatttccgg
     4081 cactccggtt caggcataaa ggaaacacta aggaaacact gcataaagcc cgcctgacgg
     4141 ctttgcacgc aatctgcttg cagat
//
LOCUS       DAWBGR010000198         4116 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5110_length_4116_cov_4.989658, whole genome shotgun sequence.
ACCESSION   DAWBGR010000198 DAWBGR010000000
VERSION     DAWBGR010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5110_length_4116_cov_4.989658"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1122 a          963 c         1133 g          898 t
ORIGIN      
        1 ctaccggctc ataaccggtc ggtcctgggt tcgagtcccc gatggcccac catatcagag
       61 ccgccagtgt ttcacgcacc ggcggctttt tcatattgac agaacgatgt cgattcggtt
      121 ataataaagg tatcggaatt cttaccaaca ggaggaacag catgaaagca attatttttg
      181 atatggacgg cgtaattttt gacacggagc gtgtcggcga tcaggcttgg cggcaggcgg
      241 ctgaagaaat gggctttgcg cagatagacg atgccatttc tcagtgccgc gggttgaacc
      301 gtgccgatac caaggcgttt ttcgagaggg aatatccaga tttggattac cttgcgtttc
      361 acaagcggaa tcacgagatc atgaaccagc tgctcgcaga cggtatgccg gtgaaaaccg
      421 gtgcggtgga actgctccac tggctgcggc aggaggagtg gcgtgtggct ctggcaactt
      481 ctactggtcg ggagagcacc atgcaccatt tggaagcggc acacatgacc gacctgtttg
      541 atgcgatcat caccggcgaa caggtggagc acggcaagcc tgatccggaa atttacgaaa
      601 tggcatgcga tgccattggg gttacggcgg ggctgtcctt tgcggtagag gattcgccca
      661 acgggattcg cagtgccgcg gcggcaaatc tgcgtgtcat catggtgccg gacttggtga
      721 agccgaatct ggaactgcgg gaactggtag taacagtaca ggaaaacctg ctgggcgtgc
      781 agaagttttt ggagaagttc ggagagcttt aatcataaga aaattgtgca ttttacaaaa
      841 aaatatgcac aattttatgc gaaatagaaa ttgacgttgc tgggatttta tgttatacta
      901 aaaatgaaat cgatgcattt ccccagactg tgaggaaagg gtttgcagtc tggggcaatg
      961 aaaaagaaag ggtgatttct atgaaaaaaa cgaaaaggat actgtcttct ttgatggcat
     1021 tgacactttc tgccgcaatg attggattat caccggtttc agcacaggaa gatgctacag
     1081 tgggaaacag tgcaagcagt ctggcacaga tgcccgaatc caaggacacc acccagaacg
     1141 cactggatct gttggaagaa gcattgcaga aggatccgaa tccggatctt gcccgcgaga
     1201 gattgtgcga agatctgggc gtttccgctc tcactgacct gacttacagc acgccgtatc
     1261 actattacga tttctatacg caggaactgg ctgttccgga atcgggagat gcctacacct
     1321 actggatctt gctgtatcgc gacggcgttt attgcggcaa tctgtcggta acgccggcac
     1381 tggggctgat tcagaaagat ctgaaaccct atgcctgcgt ggaaacggct ctggaacagc
     1441 aggacgatat gtggttcggc ggtgcaatcg ttgacggcct gccggcggaa ctggtgtata
     1501 ccggcggcgt gatctacagc ctgaacttcg gcgaaaccaa gaccgatctg gtttggggac
     1561 agctgccgca ttcggactac aacagcggca tgataattga aaccactacc acaccggcag
     1621 tcaccaccac acaggaatcg aacctgtcca ttctgggtga tgtcaatctg gacggcattg
     1681 tagacctgaa agatgcggtc ttgctgaaca aggcggcaaa cggcacagtg gcactgaacg
     1741 atgcacagct gcaaaatgcg gactgcaacg ccgatacgga agtgagtgca caggacgctg
     1801 tttctctgat gcggttcttg gtacacaaca tcagcacgct gccggatact tctgcggaat
     1861 aacatcgtct gattgcggaa tcatttgtat tcgtgaacga acgcatctct ctggttagag
     1921 aggtgcgttt tttgtttttg cggcaatcac aaatttgaat aaaatgtaaa ttttccggaa
     1981 tatctcttga cgaaactagc acatgtgcta taatagtgtt agcacttaaa aagcaagagt
     2041 gctaatttcg catttttgca attgattttg acaaggaagg agatgtgccg cggagccgag
     2101 aggctctccg gtgcaaacag aaacaaatgg aacagaaaca atttcaggca gaatccaaaa
     2161 aactgatgga catgatgatc cattcgatct atacccataa ggagatcttt ctccgcgcac
     2221 tgatctccaa ccccatcgat gccattgata agctgtattt caagtcgctg acggacgaca
     2281 gcgtgggcat gaacaagtcc gacttcaaga tcacactggc tgtggacaag gatgctcgca
     2341 ccctgaccat taccgataac ggtatcggca tgaccaaggc ggaaatggag gagcatctgg
     2401 gcacgatcgc caacagcggc tcgctccagt tcaagaaaga gaacaatctg gcagaagata
     2461 accagatcat cgggcagttc ggtgtcggct tctattccgc attcatggtt tccaagcgtg
     2521 ttacagtcaa gtcgctggca ttcggcgaaa agcaggcgta tgtatggcag tccgagggcg
     2581 tagagggcta caccatggat acctgcgaga aggataccgt gggtacggag atcacactgg
     2641 aactgctgga cgatgtggca gcagatgcag agggcaacgg cggcgaaaac tacagcgaat
     2701 tcctcgacca gtaccgaatt cagagtctgg taaagcggta cagcgattat attcgcttcc
     2761 cgatctgtat ggacatgacc aagagccgga agaaagagga ttccgacgag tacgaggaat
     2821 atgtggaaaa tgtcacattg aacagcatgg tgccgctgtg gcataagaac aagaatgaga
     2881 tcagcgaaga agaatacaac agtttctata aagataagtg cggcgattat acggatccgc
     2941 tgtgccatat gcatgtgcgg aacgaaggta ccattactta tgatgccctg ctgtatatcc
     3001 cgtcccatac tccgttcaac tactacagca aggactacga aaagggcttg cagctgtatg
     3061 ccaacggtgt gctgatcatg gatcgctgcg aagatctgct gccggattat ttcagctttg
     3121 tcaagggtct ggtggactcc gaagatctgt ccctgaacat ttccagagaa atgctccagc
     3181 acgacgcaca gcttcgccag atcgcacgga gcatcgagcg taccattaag aatgaactcc
     3241 agcgtatgat gaagaacgat cgggagaaat acgagaagtt ctatcaggca ttcggcttgc
     3301 agctgaagta cggcatctat caggactacg gcatgcacaa agatctgctg caagacctgc
     3361 tgctgttccc gtcctccaag gacggcaagc tggtatccct gaaagaatac gtagaccgta
     3421 tgccggaggg acaggacaag atttactatg ccgcaggcga gagcattgcc cgcattgaat
     3481 ctctgcccca gaccgaacag gtcaaggata agggctacga gatcctgtac ttcaccgaga
     3541 atgtggacga attcgcaatc cgcatgatgc agggctatca ggagaaacag ttccagtccg
     3601 ttctggacag caacctcgat ctggaatctg aggaggaaaa gaaggaactg gaagaaaagc
     3661 aggaatccag caaggacatg ctggaagctg tcaagaaggc tctggagggc aaggtcggca
     3721 acgtcaagct gtccggccgc ctgaaatcgc atccggtctg cctgtccagc gagggcgaac
     3781 tctctatgga aatggcaaag acgctgaacg caatgccggg caccgaggga cagaagttgc
     3841 aggcacagac tgttctggaa atcaatccgg aacacccgat ctatcagcgt ctgatggaac
     3901 tccagactgg cgacagtgca aagctggacg actacgcaaa gctgctgtac gatcaggctc
     3961 tgctgattgc cggcatgccg gtagaagatc cggtggcatt ctccaatcgt atctgtgact
     4021 tgatgtaaaa ttgcaggctg tccaagcctg agaaaacact gaataaaggc aataccgcac
     4081 aaagagcgtc tggcgacttt gcacgccatc tgcttg
//
LOCUS       DAWBGR010000199         4027 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5258_length_4027_cov_6.091390, whole genome shotgun sequence.
ACCESSION   DAWBGR010000199 DAWBGR010000000
VERSION     DAWBGR010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5258_length_4027_cov_6.091390"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          935 a         1177 c         1012 g          903 t
ORIGIN      
        1 gctggcagcc gcaggctgac tgagggattc ttctgtgtaa ctgaaacatt aacaaaagat
       61 ttcacagaac ccctccacca acttgcaaac gctgcatcaa ataaaggtta accacctgca
      121 caagaaaact cttgaatacc aacattaccg gcaacaaccg gcgttggcaa gcccaaggcg
      181 gacagcgagg aacgagcgaa ttccgccgga cggtttcgac acggctgccc tagaaaatca
      241 ctgtcctcgg gcaatcctgc ggggcgatgt gggcatcgcc ctctacacat tccaagaggt
      301 tcttccatga agtctgacat atcaggggag aaccttttac aaggcaaagc acaaccccac
      361 ccactgtttc acgtgaaaca aaaaaccggc agagcctgtc ccctgccggt tttcccattt
      421 ctaaaacttc cctagccaat caagcccagc ccgatgtaaa acgccgccgc acatgccgcg
      481 aagcagccgc acaacgtcca gccaatgccg cagagccagc ggcacacggg ctttttcccg
      541 ttccggaacc gctggaatgc cagccccaga aacactgccc cgatgacaca agtcggcgta
      601 atgtagcggt agttcatgga acaggtaaaa ggttccgccg cggaaagctg ataaaatccg
      661 cccatcagca ccgcgtagaa cagcaccaga aacagcttcg gcagccgtcc gccagtctgt
      721 tcccgtttgc cgcagagcca gagcattgcc gcaaacgctg ccgccgccag caccacattg
      781 agccagaaca gcaccacgcc ggtgaggatt cgccacaggc tgcaatccag cgtgtactcg
      841 ttgatatact ccccgaacat ggcgtttttc agcagggcga tcagcgggtt atactcgttg
      901 tagctgccgc cggttcgctg tacccactgt tcgaaaacgc ttgccacctg atgcggcgaa
      961 aagtccagga tccgtgagag aaacgactgt tcccccagat actgcacaga gttttccggc
     1021 atttcctgca cataggtcag cggtacgccg aaccgcacca gattccgcac cggataccac
     1081 agccccagcg ggaaacagat cactgcaaat ccgccgagct gcccgaggat ccgccagttt
     1141 ctctgccgca gctgccgtac cagcaccacc agaaagactg ccgccaccgg tattgccacc
     1201 attgccgccg aaagctttgt catcatgccc agcccgatgc agagggcaat tttcacgatg
     1261 ccctcccacg tctgtttctc gtaccatttc agcgtccaca gcactgcccc cagaatgaac
     1321 accaccgaca acgcatcgtt gttgatgctg ccggaaaaga gaatgaagct ggggtgaaat
     1381 gctaccaccg ccagcggtgc atacaacgcc atgccctcca gccggaagtg ccgcagaatg
     1441 cggtaggcag tgatgacgat cgccatggcg tagaacaggg acagcgtttg cagggactcc
     1501 tgagctgcgg cattgctgat gccgaacacg tgttccgaca gccacagcca cagggcagag
     1561 atcatgtgat gcaggggtgg gtggtagaat tgccagtgtc cccgcggatc aaaatccggc
     1621 agctggtgat gctgcaacag ataggtgatg tagccggcat ggttgtccgc atccccgaat
     1681 tttcccacat cgtgctgccg tcgggtaatg ggcgtgtagc acacgtagaa aaatttcagc
     1741 agaaagcttg tccccagcag gagcagtgaa acccgccgcc gctggttgtg gtactgcttt
     1801 cgggtgagcc agaacagtcc gcttagcaca gcacagccgc cgaccagcac ccacagtccg
     1861 gagtgtctgg cttcgtgata acaccacgcc ccagccagca ccagcagtgt atacaccaca
     1921 ctgacgcaga ccgccgccgg tttcttctgg ggctgatacc gcaccagccg ccacaagact
     1981 gctgctcccg ccgccagcag agccagcgtt tccagaagca cggcattttg tgccagatac
     2041 gcttctcccc cgaagtacag tgcatgcagc agcagacaca cgaacagact gcacagatag
     2101 gggtgccgtt ccagcaggtg aaaataccgg ttggactccg ccgccggcag gcagatacag
     2161 ctgcaccggc agccgtgaga acgattgccg gtgcgtttgc tgtttttgga aaagagctta
     2221 cttctgctca tgatattcct tttcctggtt gacgttccag accggaccct tgtccgtgga
     2281 atcgtgcttg atcgcttctg ctgcgtgaat ggcggtcagc atggagtgat ccatattatt
     2341 gtagcggtgc tgtccgtttc tgcccacgca gaacagattc tcaaaggtgt tcagataatc
     2401 cactacctga tcgaactgtg cataggtgtc gaaatatgcc ggataagcct tcttgacttt
     2461 ttcgcggtgt gcgtccagta cggctccctc ggagataacg cccattttgg tcagctcctt
     2521 gacggcgaag tccacgcact ccttgtcgga catgttccag aacttgtccc cttcggcaca
     2581 gaaatactcc agaccgatcc agacggtgtc ttccggcttt tctaccatat aaggcgacca
     2641 gttattgaag atctggatac gacccagctt gacacccttg tcctgtacat agatccagca
     2701 atccggtaca atattgccca gtgtcttagt cccagtctga ttgaccagat tcagcttgtt
     2761 gaccagcagt cccacggtga caaagtcgcg gtacggcaga ccggcagcga tccgctgtac
     2821 ctcggtattc tggcagccgt ccatgccggc gatcagatcc tggatcggca tagaggaaat
     2881 gaagatgtcg ccggaaatgg tcttgctgcc ctccggtgtt tcacagacta cagaagtcac
     2941 cttgccgttt tcggtgtgaa tggtcttgac gctgtgatgg aacaggatct tgcagccctt
     3001 ttcctcggct ttcttgccga ccagttccca aagctgtccc ggaccatact tcggatacca
     3061 gaactgttcg atcagagagg tttccgcatt tttgttgttc ttcttgccgg aacgcttgga
     3121 gatcatgtct ttcagcacgg cacggataga cagccccttg acacgctgag caccccagtc
     3181 tgcggagatc tcgctggggt gtctgcccca caacttttcc gtgtagccct cgaagaacat
     3241 gctgtacagc accttgccga accggttgat gtagaagttt tccagattgg tttccggctt
     3301 cttggagatg caggaaccga ggtagctgaa gccagccttc atggttgtgg caaagcccat
     3361 gctctgaatg gtgttcttgt tcatagtcac cggataatcg aagaagtgct tgttgaaata
     3421 gatgcgggaa acgcggtcac gcaccagcat gactacgtct tccttttccg gatcagggcc
     3481 gccggcttcc agcggctttt cccgtcccag ttttttatca tcaaaggacg gctgtccctg
     3541 aatgggcatc aggtctgacc accatttcat gacggtgtcg tccttggaga aaaagcggtg
     3601 tccgccgatg tccatgcggt tgccgtgata gcgaaccgtc tgggagatgc cgcccaatac
     3661 gccgctttct tccagaatgg tcacttcgta ttcgtccttg gtgtctttca gcagttcata
     3721 cgctgctgtc agaccggcag gaccgccgcc taaaatcact acttttttca taaaagaaaa
     3781 atcctttctt ttttagcagc actgcataag ctgtgcatag tactgctttt taattttcaa
     3841 agcatgcttg atgctgtcag atgcatgcga aagaacgcag aacatactct atatattata
     3901 ccacaatttt cgtagtttgt caatttattt ttctgtaatt cctcattttt tgggcagtgt
     3961 tgccctaggg caataccgca caaagagcgt ctagcgactt tgcacgccat ctgcttgcaa
     4021 attttcc
//
LOCUS       DAWBGR010000200         4004 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5291_length_4004_cov_5.133452, whole genome shotgun sequence.
ACCESSION   DAWBGR010000200 DAWBGR010000000
VERSION     DAWBGR010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5291_length_4004_cov_5.133452"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          835 a         1145 c         1038 g          986 t
ORIGIN      
        1 acccgaaggg cgtgccttgt gctgagctgg caagccgtag gcttgactga ggggttacct
       61 gctacagcag ccaaccggtt tcacaaggag ttacgcttct ttttttcttt actgaaaaga
      121 aaaaaagaag caaaaaaaga aaagaattcg cctacccttg tactgtatgg caatgcacca
      181 cccgaagatt acacaccaac aaaactcccc cggcggggca aacaggcggc tgctgccgcc
      241 gtaacattac ccttttcaca gttgaaattg gggcgtaggg tcagcggcga cccgccgccc
      301 gccgcaacgg gagaatattg ctctcgaaat agcatacaaa aaaacaacac cgcacaaagt
      361 ctgacgaaca gctttgtgcg gtgttttttg aaataatgca atggcttact tgctggacgg
      421 catcaccttt gccaatgccc ctgccatacc ggacagcggc aaggtctgga gccagatgcg
      481 tcccggaccg gtgaccttgg tgttgaacag tccctcgccg ccgagcagca tgttgcctac
      541 acctttgacc atttccacgt caatcgtgca ggttgcttcc atggcagcca gattgccggt
      601 gtccaccatc atggactgcc ccggagccag gtcatatgcg accatgctgc cgtctacttc
      661 cagaaacagc atgccgtttc cggagaattt ctgcatgatg aagccctcgc cgccgaagaa
      721 gccgcctgcc agctttttct ggaagaacag ctcgaagtga acaccgactt ccgaagccag
      781 aaaggcagac ttctgtgcca caatggaacg ctccggagag atctgcaccg gcatgatctc
      841 tcccggcact gcggaagcaa aggcgatcat gccctgtccg cccttggcgg tgtagtggtt
      901 ctggaacatg gattcaccgg tgaacgcacg gctgaacatc ttgcccagtc cgccgccctt
      961 ggtctgcatc tccatgttgg gggtcatcca tgccatagcc cctttctggc ataaaacggt
     1021 ttcctccggc tctacggtca gaacgaccac cggatagggc tgtccctgaa tctgatactg
     1081 catgttgtaa atcctcctgt atatttttta gggcaaatgt atgaatgtat gctccaatta
     1141 tccctgtaat ccctttgcac cgatgtgcac ttctccggcg tggagggttt ccacgttgcc
     1201 gaagctgtcc cgcacgacca gaccgcagtc gtcggcaatt tccagtacag ccactttttt
     1261 gacagtaccg tcatcttttg tcagttccac ggtctttccg atgaggcagg aacggcggcg
     1321 gtattccgcc agaaaccgcc gctggggcag ttcccgcagc acctgctcca tttcatagag
     1381 cacagttgcc agcagctggg cacggcagac gtgaatgtcc ggacgggctt cttccagcga
     1441 agtgaccgtg tgccgcagtt cctccggcat ggcaatgctg gtgttccgga cgttgatgcc
     1501 gatgccgata atggcatgtt ccagcttgcc ggattccaga ctgacaccgc cctcggtgag
     1561 aatgccgcag catttctttc cgtccagata gagatcgttc acccacttga tctgcatttg
     1621 cgtgccgtat aaagcgtcaa tggctctggc ggcggcgaca gcagcacagg cagtcagcag
     1681 cggtgcgtcc tgtagggaaa ccgcctcgga caggatcatg gaaaaataca gccccgaacc
     1741 ggcaggggat acaaaagtgc gtccttgtct gccttttccg gcagtctggc agtttgccgc
     1801 caccaccgtg ccgtgtctgg catgatcccg caccagcgtt ttgcaggtgt tgttggtgga
     1861 gcccagttcc gaatagacgt ataacgggtg tcccagaatc cggctgtgca gatacgggcg
     1921 gacggattct tctgtcagca gatccacgcc ctgtgccagc cggtagccgg cctttttctt
     1981 ggcttcaatg ggaaagccct cgtcctgtaa caggcggacg gctttccaga cagcgtttcg
     2041 gctcacgccc agcttttgtg ccagcgtttc accggagaga tattgtccgc ttgcttcggt
     2101 caggcaggcg gcaacggcat cttttactcg catgatgcat aatgctcctt tgtttgtctt
     2161 tgcaaaaatt catatttttc gcaggctttc gatattagta ttataaacaa ttttcatggt
     2221 tttgtcaagg gaaaatttac acgcagattt cgtgaaaagc cgtcaggcga caggctgacc
     2281 gccgcaaaaa acagccgtcc ggaatccgaa cggctgttgc tgttatgaca ataccgtaca
     2341 aagattgtgt ggtattgcct tatgtttctt gtaaatgctt tcgcaggatc aataagcctt
     2401 gccccacagc accatgtgct ttgccggctt gccgcagcag acacacttgt ccgagatgtg
     2461 cttctgctcc agcggaatgc agcgggaacc tacgccggta acttccttga ccttgtcctc
     2521 gcaggcttcg tcaccgcacc acattgcttc tacaaaaccg tcgccctttt cttccagagc
     2581 cttggtgatc tcgtccatgg tctggcagac ataggtcttg tttgtccggt tttccagtgc
     2641 cttccggaac atgccctgcg gcactacgtt ctccagcagg tcgctgacgg tgtcgcacag
     2701 agcctcatag ttctcgtcca gagcgaccgt ctgcttctcg ccgttgaagc gggtggcaac
     2761 gacacactgt ccggcttcga tgtccttggg gccgatctcg atccgtaccg gaatgccctt
     2821 catctcgtac tgggcaaact tccagcccgg agagttgtcg gaatcgtcca gctttacgcg
     2881 gattcccttg gcagccagag ccttgtacag ttctgccgcc ttgtccagaa cgccttcctt
     2941 gtgctgggca atgggcacga taactgcctg taccggagcc acagccggcg gcagtaccag
     3001 accgctgtca tcgccgtgtg tcatgatgat cgcaccgatc agacgggtgg tcacgcccca
     3061 gctggtctgg tgcggatatg ccagcttgtt gtccttgccg gcgaactgaa tgccgaatgc
     3121 cttggcgaag ccgtccccga aatagtggga agtacctgcc tgcaatgcct tgccgtcgtg
     3181 catcagagcc tcgatcgcat aggtagaaac tgcaccggcg aacttgtcgc tttcggtttt
     3241 cctgcccttg actaccggca ttgccagaca cttttcgcag aagtcggtgt atacgttcag
     3301 catacgctcg gtttcttcaa ttgcctcctc tgcggtggcg tgaatggtgt ggccttcctg
     3361 ccacaggaac tcacgggaac gcaggaacgg acgggtagtc ttttcccagc gtaccacgct
     3421 gacccactgg ttgtacagct tcggcaggtc gcggtagctg tgtacaatgt ccttatagtg
     3481 atcgcagaac agggtttccg aagtcggacg gacgcaaagc cgttcctcca gcttttcgct
     3541 gccgccgtag gtgacccatg ccacttccgg agcgaagccc tctacatgat ccttttcctt
     3601 ttccagcagg ctctccggaa tgaacatggg catgttgaca ttcacgtgac cagtcgcctt
     3661 gaacatgccg tccagaatcc gctgtatatt ctcccagatc gcatagccgt agggacgaat
     3721 gaccatacag ccctttacgc tggtataaga tacgagttct gcctttttgc agatgtcggt
     3781 gtaccactgt gcaaaatcca cgtccattgc cgtgatctct tccaccattt tctgattctt
     3841 ttttgccata gtatcctcct gtggttgttt ccggtgcagc cgcaccgtca gtctgttttc
     3901 agtatagcat attttttgcg gaattgcaag gggattcttg gatttgtaag gaaacactgc
     3961 ataaagcttg tgcgtaagat gcgtagtcag atttttcggc agat
//
LOCUS       DAWBGR010000201         3983 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5319_length_3983_cov_2.985489, whole genome shotgun sequence.
ACCESSION   DAWBGR010000201 DAWBGR010000000
VERSION     DAWBGR010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5319_length_3983_cov_2.985489"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3001..3010
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1324 a          685 c          789 g         1175 t
ORIGIN      
        1 aactaatgaa tgccgatccg gaacaattga taaagtcagt caaaagcatt cgatgtggta
       61 acatgagaat caagtttcaa atgtatgcct taaagcataa catatcatat ttgcggcaaa
      121 tgcagcagga acatggaagc attgatgcct attatcattc aatgactaag tatgacttag
      181 ttaagacgtt agataataat ttaaaagaaa tgagcgccac gcttatttct gaatacttac
      241 cgagtgtagg tattgatctt cctaaaccgg atagacatat tcaacgcata cttggcagcg
      301 agagacttgg attttcaagc cgaaaaatgg catctattca agagtgttat gaggttattc
      361 atgattttgc agttgcaaca tctgaacacg aaaggtacat tgacgttatt ttatggtctt
      421 attgtgcgga aggtgaaaag aatatttgtt gtgcaagtcc tcggtgtgag aaatgtgtaa
      481 ttagcgagta ttgcaactac aaaggaaaaa gtgaaaaata aatcaagctc taagaaatta
      541 aattttatgg gaatattaaa catccacggc tacggcggtt cacctcataa cgcagcttat
      601 gccgcattac aagcaaatag aagaatgatt atttgaaaag aacggtttct caatacaaga
      661 agtcattatt ataaggagta attacaatga atcagatcac aactttcaga gatacatatc
      721 gctttctcaa agatacacat cgctttctct ccaactttta tcagtatccg ttcacttaca
      781 agggacttgt gtatcccaat gcagaggcag catttcaggc acagaaatgt tcttcggatg
      841 cggacagaat caaatataca ctccagaaaa gtcctgtcga agccgaacga atgggcaaga
      901 aagagcctaa tcttccgtca aactgggacg aaatatccta tgatatcatg tttgatattc
      961 ttcgtgcaaa gttttccgta ccggagttgg ctgaaatgtt agagtctacg ggtgacactt
     1021 atctggagga gatcaataac tggcatgaaa atcgctgggg aagatgcacc tgtgaaaaat
     1081 gcagagaaaa agagggcaag aattggcttg gagaaatatt gatgcgtgta cgccatatga
     1141 atagaaccaa atatatgcat atagaaaact tagaaaaaat cacatcacat ctgaacagac
     1201 ctgctctcat tgcattggct gcacgtccgg gtgtgggaaa aacaactctt gcaatgaata
     1261 ttgcaattta tgaagcagag cattacaaca agaatgttgt ttacttttcg cttgaaatgt
     1321 atgaaaaaca attatatgtc aattataaca tacaaggtaa atcatccata cgtattattg
     1381 ataattgtct taatgattat cttacagtat cacagatgaa agaaaaactg caaaatctgg
     1441 gaaatgtaga tttagtcatt atagattact tgcagttgat aaaatctgaa tccgaacaca
     1501 aaaaacggct tgcttggctc tttgatattg gaagagaact gcatgatctt tcacaggaac
     1561 gaaaaattcc ggttctcttc ttatgtcaac ttcccagaaa tatagactat cgtacagatc
     1621 aacgcccgtc tatgcaggat tttagaaact ggatcggtct tgaacaactt gtagatattg
     1681 agatgctcct ttatcggaat tcttatccga aatgcggtga accattttac tatcattact
     1741 atcattttga tcccattata gaatgcaata ttgctaaaaa caaatatgga gaaaccgaaa
     1801 taattccatt gaactgccga cttgatttca aagtattaca atcaaataat gaagtttaat
     1861 taggagctga ttatatgatt tttatcacag gcgatttgca tgggtatcat gatatagctg
     1921 tttttatagg tggtctatta tgaatattta tccgtaccat tcggcttatc ctgcctatac
     1981 ggtaaaaggt aatgatatat atgagtacca ttcagtttat cctgcttata cgataaaggg
     2041 caatgagata tatccgtacc attcagctta tcctgcatat acaataaaag ataattgatc
     2101 gggggtcaaa atatgcagta tatcaaacga ggcagacagt atacttttga tggttttgaa
     2161 gtcaccaaat tcaacagcaa agcctttgat gccgcaaaga aatttgctga gaacgctgat
     2221 tccaagcctt tagcgttatt cggcggtaca gcaaccggca aaacgcatct gctctatgca
     2281 gtcaaaaaca tgattgagca gaatgagcct aagttgacgg tgatcctcac cacagcagca
     2341 gaaatgcgaa cttcgctggt gaatactctt caaaacgacg gtacagcgga gcagtttcgt
     2401 gaaaagtata tgcaggcaga tgttctgttg gtagacgata ttcaggagct tgcacgtaag
     2461 aaagcgattc agaatgagct gattttgctt ttcaattggt tctatgagtc gggtaagcgt
     2521 ttcatgatga cttcttctaa gaaggaagct aactgcggaa tgcgtagccg gctggtcatc
     2581 cgttcattct ggggagattt tgcagtaatt tctaaggcat atatccatgc acagtttccg
     2641 tctgatcatg gacgaaccga aatacgtaac actctcttag aaaacggcaa atatctaatt
     2701 gatgactttg aactcgacag cttcaaaacg tattataaaa atgtttggaa atacttcaaa
     2761 agatacatcg gactttttga tgtggatcgt aaagatattc agctaataaa caatctgaat
     2821 gatatcctga acctattact atatgaaaca cctttagagg aacgggaacg ccagacttgt
     2881 attttattca caaaagcatt gctatacacc atatcaacat acgatatttg tatattcaga
     2941 ggcggcttct acagcgaagg cgttctttac attccattgc cgcatcatgc aggaacaagt
     3001 nnnnnnnnnn gaacaagtga tttagaaatc aaaattgatg agtttgatga ggaatttaat
     3061 aaattctgtg aggaatatca ggaatatgag gatttctgaa atttttatca aaaagaataa
     3121 gaagaattgg cgttttgatt tatgatcgga ggtattgatg aacgtatctg tttattcaag
     3181 agaagccatc gaattcatta ttgcagccgg taactttccg aagaatactg ctgttatcag
     3241 cttttacgat ccggcgataa agcgtatcga cgaggactac tcccacgttg attacagcgg
     3301 tgtctgcaat acagtttttt acagcgaact ggacgacctt gaccttgatg tactgagacg
     3361 caagggatat acatacgata cttatttccc cgaagcacct gatattgccg ccttcatcta
     3421 ccgtgcatac gatattggta tgaatatcat atgccagtgc gaatacggtc agagcagaag
     3481 tgctggttgt gcagcggcta ttttggaata tttttatcaa aacggtattt ctgtatttgc
     3541 aaattataat tattatccca atcaggttgt gttccataaa atttatgatg cacttgtaaa
     3601 aataaaccct gcatattgca ataaatatca ttcaagcaaa ttaagcacag gcattgaagc
     3661 tttgttactt aagcaatctt tagaaaaaaa tgattgttat cattcattgg aagaagcata
     3721 ttttaggctt tgtcatggta ttgctaaagt taatgtttct ttgcatatcg ataatgcaaa
     3781 ggtttgtcac ggaaaaagca tgggagaaca taatgttggt gtgaaaacaa tttttaaata
     3841 ttacgacaaa gagatacctc ttattattta tatgcctaaa ataataaaat atatcaagtc
     3901 agataatcag gagtattatt taggaacaat aggggcttca attgctcgtt tcagaagacc
     3961 tcatgcttcg gtttcctttg ata
//
LOCUS       DAWBGR010000202         3794 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5635_length_3794_cov_6.722118, whole genome shotgun sequence.
ACCESSION   DAWBGR010000202 DAWBGR010000000
VERSION     DAWBGR010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5635_length_3794_cov_6.722118"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1001 a          844 c         1046 g          903 t
ORIGIN      
        1 tgattaccaa cagcagcgaa attatcggaa aaggattccg caataccata taaaacaaaa
       61 caaccccgca caaaatttgc ctttcggctt ttttgtgcgg ggttgttttc agttatgaaa
      121 ggaaatattc tttcgcaatt agtcgttgaa gtaacgcttc aggaagccgg caaatgcaca
      181 gccgtgtctt gcttcgtcct ttgccatttc gtgtacagtg tcgtggatcg cgtccagatt
      241 cagctgcttt gcctttgctg ccagagcctt cttgccggca catgcaccgt tttccgcagc
      301 gacacgcatt tccagattct tcttggtgga atcggtaacc acttcgccca gcagttctgc
      361 aaactttgca gcgtgttctg cttcttcata agcagccttt tcccagtaca gaccgatttc
      421 cggatagccc tcacggaaag cgacgcgagc cattgccaga tacatgccga cttcggagca
      481 ttcgccgttg aaattttccc gcagaccctg cacgatttct tcgtctacgc cctgtgccac
      541 gccgacaacg tgctgatcag cccatgccag attttcttcc ttctgctcca cgaacttgtc
      601 tgccggtgca ccgcagagag gacatgctgc cggagcagat tcgccttcgt atacataacc
      661 gcaaacagaa catacaaact ttttcatgag taactacctc cataaaatat catttgccag
      721 gagatctttt tgtccccctc agcttgtctt tattatagca taagaataat tccttgtcaa
      781 gatgtattct caactagtta caaaaaatta acatatagat ttttagggca ataccgcaca
      841 aagattgtgc gacagatgcg tgcgggaaga tacgaaacag gctctgaagg gaaatttgca
      901 agcagatggc gtgcaaagtc gccagacgct ctttgtgcgg tattgcctta gggaaacacg
      961 gattaaagct tgtgtatagg attcatcgtc aggcgggctt tatgcggtat tgccttatgt
     1021 cacttgacac acttgttatt ttgcattata ataagtatca cgattacgga gaaatatggg
     1081 caactacgca caggctctca gaaaggggaa cggtgatgaa aaagagaaga tttgactggc
     1141 tgcgaaagac cagttcggca gatgtgatga acccgaaaaa ttatgaatat cggctgaaac
     1201 agctggacga tgacaatccg gcagaacaga aagaagcata tacgtggttt cagaagcacg
     1261 cagaagaagg cgatgcatct gcacagtatc agaccgcaat gtgttttctg catggagttg
     1321 gtgtggaaaa agatgaggaa caattcctgt attggctcac aaagtctgcg gaacaggagt
     1381 acggcgaggc actgacgaca atggggattc attatttcta tgattttgat gccgtagaag
     1441 aaacagaaca ggggattccg tggatggaac gtgccataga acagcacgat gataatgcgg
     1501 ctgcgatatt gggtgtatat cttgtggaaa ctccgaaaga ggactgccat gaggaagaac
     1561 gcaggcaccg cggctttttg ttgctgcaat cttctgctct ggaaggcaac cttgaagatc
     1621 atctgcggat acaggcgttt tttgaattag ggcattgtta ttattacggc attggaacag
     1681 aaacaaatat tgaagaagct tatacgtggt atgcccgtgc tgcggaatcg gggagtgaag
     1741 gtgctcaata tttggcggca tctttgatac tggacaagcg gtgttctgaa acggataccg
     1801 caaaagccat tgcgtggctg cggcaggcag cagaacaggg ctgtatgtat tccacgatgc
     1861 ggttagggga atgctatctg gaaggcaacg tggtgcccaa agatgcggaa gaagcttttc
     1921 agtggtttca gaagtcagcg gagcttggca accccgctgc ggtcaatatg gtgggacgct
     1981 gctatatgga cgggaacggc gtagagcaag atcaggaaaa ggcatttcag tattttcaga
     2041 aggcatcaga agctggtgac gaatctgcta tgtttaatct ggcagactgt tatttcaacg
     2101 ggctgggaac ggagcagtcc gaagaacagg cagtcccgtg gtatcagaaa gccgccgaag
     2161 cgaacatttc cattgcccag tacatgctgg gaacctgtta tcattttgga aagggcgttt
     2221 cgccgaatga agaactggca ctgcactggc ttcgaaaagc tgctgaacag gactatgtcg
     2281 gggcacagta cgcattgggc acgcttctgt tccggaatga ggatagcgga gaaaacgatg
     2341 ccgaagcagt ccgttggctg gaacgtgccg cagaactggg cgatattggt tctcaggtga
     2401 agctgggcgt ttgctatcag gatggataca acgtgctgga gcagggaaag gtctgcttca
     2461 aggaggacat ggaaaaagca gtggaattgt ttcagaatgc tgcggaacag ggtgatgcag
     2521 aaggacaatt ccagcttgcc aacagctatc gtttcggttt aggcgtgaaa aagaatgtgc
     2581 cgaaaatgct gtactggctg cggctgtctg ccatgcaggg ctatgcagag gcacagtatg
     2641 cactggggat ctgctatcag gacggtatcg gtgtaaaagc ggatatagag aaggcaatgg
     2701 actggtatga gcgttccgct gcccagaatt atcccgatgc catttgttcc gtgggcatgt
     2761 actatctgga cgaggaggac gatgtggaaa ctgccaagcg tctggtgtat aaagcggccc
     2821 agatggactg tccggaagca cagtatttaa tgggtatgtt tctctatgag ggtgatgaca
     2881 gcgaagaaga tgaggaagct gtcgcatggt ttcgaatggc agcacggtat cagaatcacg
     2941 gcagtgccat gggcatgctg ggctattgct atatgaacgg caccggtgta gagcaagacg
     3001 acgaaatggc aagggtctgg ctcaaaaggg cggcagagtg tcgggattat gatgccattg
     3061 aactgctgaa aaattactac atgatcgacg actacgacga tttcgaatac gaagattacg
     3121 gcgatgagcc ggagtatgat gacgaggacg acgactggga agattaaagg caataccgca
     3181 caatctttgt gcggtattgc ctaaaaccgg aatccccgca tatcctgtcc ctccagtgtc
     3241 agcaggtcga tcttccggcg gtcattgccg aaatagccgc ccagcttgcc gttggcaccg
     3301 accacccgat gacagggaat gaaaatggca atggggtttc gtttcatggc accacctacc
     3361 gcctgtgcgg acatcttggg aatgcccatt tcccgtgcag cttcctgtgc cagcatgccg
     3421 tatgtggcag tgcagccaaa gggaatctgc tgtgccagcc gccagatccg cagctgaaac
     3481 ggcgttccag ccggacggag caggggcgta gtttccggag ctttgccgtt gaagtaggtg
     3541 tccagacagc ggcacacgct ttccagaaac ggtgtggctg actgcggcag cgttgccggc
     3601 acatcaaaag ccgcttctgc cagagcattg tcctcggcgg taagacggat atttcccaga
     3661 ggggaatggt agatcaattg tgtttgcata agatgttcct ttctttacat gatatacaaa
     3721 tacagcccga gaataacagt ggcaataccg cacaaagagc gtctggcgac tttgcacgcc
     3781 atctgcttgc aaat
//
LOCUS       DAWBGR010000203         3786 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5652_length_3786_cov_3.285446, whole genome shotgun sequence.
ACCESSION   DAWBGR010000203 DAWBGR010000000
VERSION     DAWBGR010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5652_length_3786_cov_3.285446"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          898 a         1001 c          818 g         1069 t
ORIGIN      
        1 gactgtcatc catgaactgc ggaaacagca agtggggagc gatagggaca tagtgcgtat
       61 ctactgcaaa gcggctgtat cgtctggcgt tttcgatatt gtcattgata cagccatggg
      121 aatagggaga acaaatgtat accagcggtc gataagcggc agcttttttc gccctgcgtt
      181 cctctcgttc aatacggctc agtgcttcat aagcagtgag atcaatgtac ccttcggcat
      241 tataccgatt catgcaatac tcctttcagc cgcttcagtg tgcaggcatc gcagtaaaca
      301 gcactgctga aaatgtcaaa gttttctgct gtccagaaga tactcagatc aaccggcact
      361 tctgcaccgc actgcgggca gtggcagtat acgttttcgt tgttgatctc cacggagata
      421 ctggtggtgt cattcaagtt ttctttgatg taaaacatat ggaatcctcc taatctttct
      481 tgtaaaagct gcattcatat ccgtctgccc gaagcagcag tccctttgcc cagtctggcg
      541 ttatcgccat ctgctgacag atctcatcca gctttgtatc tttcgggcat tcgatgatca
      601 tttcatcgtg aatatgaccg acaatgaagt attgtgatag tgtctgcatg gaatagaaga
      661 gcagatcccg tgcggttgcc tgaacaatgt tttcgaccag cttgccggag taagtttcca
      721 agcgttccca ttttctgccc gtgccaatgc cctcataagt gatagaatca ccgccgaagc
      781 gattttcacc gatgcgtggc ttgacatatg ccaaccgtct gccagacagc agcttgataa
      841 acagaaaacc agattcataa gagaagtgaa tgccgtgggt ctctgtttcg gttttatccc
      901 gcacagcttt gatggctgca ttttctacat cccaccacaa ttgtacaatg tgtggagaag
      961 cagttcgcca gtccgtcaca atttgcttta gttctgcatc ggacatttcc gatccaccca
     1021 tggctttcat tgctccgacc gatccgccgt agccgcatgc caattctgcg accttgcctt
     1081 tctgccgaag atgcccgttg atgccatgct tgactactgg cacaccgaaa atcttagatg
     1141 ctgatgcaca gtagatgtcc ttgccctctg cgaacgcctg cattcgccac gtttcaccgg
     1201 caagccacgc aatcactctt gcttcaatcg ccgagaaatc tgccacgagg aatttgtagc
     1261 tgggctttgg cacgaacgcc gtccgaatca gctgtgagag cgtgtccgga acatcttcat
     1321 acagcagttc taccgcctct aaatcaccag acttcacaag cttccgtgca tcttccaaat
     1381 cgggaagatg attctgcggc aggttttgca gctgaatgat acgaccagcc tcgcgccccg
     1441 tacggttcgc tccgtagaac tgaaacattc ctcttgcacg accatccgag cagacggcat
     1501 tctgcatagc ttgatatttt ttgaccgagg attttgatac ttgttgtcgc agtaacaaca
     1561 cggttcgcaa gtccggcgaa gcgtttttca gctgttcctg tacttctttt ttgcccaacg
     1621 attctaactc cagtccgtgt tctgccagcc attgtttcat ttgctgaaca gagttcggat
     1681 tgtccaagtc ggtcagatct ttcagttgat gcagcagctt gtctttcgtg agcgagtcca
     1741 tgcggattgc ctgttgcacc aactgtaaat ccagttgtat tcctcgatcg ttgacggtct
     1801 ggtcaagatc gtactctcgc cagacaaatt ccggcacagg aaaccgagca attttttgtt
     1861 caatcgcttg ttccgtctcc acatcccgtt ggttgtatgc ccgaaagacg ctccacttct
     1921 ttggagcatc ggcaggtgca tgaaacaccg gaatggcatt tacatggtca tacggcacgc
     1981 agaaatagcg aatcagggct ttcccctcgg acattttttg ttgctgtaac tgtagaactg
     2041 ctcccacgcc ggcaaggctc agcggcaagc ccagataggc agctgccacc atcgtacacc
     2101 gccatgcttt cgggtttaga tagttgccgc aggcatcctc cggtgagccg taggagacga
     2161 accgttccgg gtagtttcgc cgcagccaga ccgacaggca gacccgttca aagctggcgt
     2221 tgaaagcgtg tttctggatg cggtcatcgg tcagagcgtt caggatttct tcaggcagat
     2281 gttcgccgca ggcgaggtca accacctgca ccggggcatc gtccacggaa tatgcaaaaa
     2341 gcaaaatatc aaaatacggg gaatccgcat agcggtaaac cccggctttt gtaatatcca
     2401 catcactttt tgtttctaag tcaatcatca atttttgcat tgttacacct attacccacc
     2461 cgaacggata ctccgtcagt cgcccacccg acatttttgc ttacttgtga ttcttgaaac
     2521 gatcaatcag tgcgacaacg gaaattgctg cccagcagaa cattgaaatg caccaaagaa
     2581 ccgcaataac aacggaaaga attgcctcca tttttctcac cgtccttatt actaaattgc
     2641 cattttagtt aatcaaggaa atcgtcactt tcaagagcat cgaaatcatc agcagcattg
     2701 gtatgtccac taagcggttc accatcccgt accttctgaa tattgcccaa accgcaggca
     2761 atgcccttat ttccgttgct gttaaacgca tagaatgtta ctgcaactct tgcatagcag
     2821 ccactgtaga cctcattctg atcgagaatc ggctgtacct gctggtcaac gatctgcgga
     2881 ggagtagtgc tatttgcatt gacaaaatag cagtctttgt acacttcatc ctccggacgt
     2941 tctgcatcgc catctctcag cggcaacttc agagcagcct tactcggctt ctttcctccg
     3001 aactttccaa tgccatcttc aatggcagca tcaattgcag tctgaatttt tgcaagagtt
     3061 gccttatcag acttcggaat cagcaaggaa acactatact ttgcggcacc atctttgatg
     3121 gatttcggtt cccagatgtt tgcgtaactc aaacgcacag ttcctgtaat cacttttgtt
     3181 tttctttcgt ttgccattta tttttcctcc tgtattgttt caaaatcttt ttctgcggaa
     3241 ttccaagtcg gtcgcttgtc cgaaattggt acaagtgcag gcttacccgg cggtttgtaa
     3301 gtataatctc ccagaagctc atcaaacttt ttcttaccac cgagtagttt ggtcattgca
     3361 gtaactccca gcaattccgg ttcgttgtac ggatttttcc cataggactt gaccttttca
     3421 atgacttttg cctcatcggt atactttcga ttcgaccgac cttccacaac tttgtaccca
     3481 ttccactgtt tgccggaaat tgctcgctgc aaagcatatt ccttgatatc ggatgcccat
     3541 gaaaccaatt gatcagcttt ttccaatact gcctcgattt cagtatccac cagcatttcc
     3601 gggggagcga agtcatactg tgccagctga agattgtatt ctgcacgttt tcggcaagtt
     3661 gccttcactt tacaaaaccg acagtgttca ccagcacaga aatctccctc gcctttggat
     3721 gcaagttctg ctttcgtttt caattctgtt tctgcccaat gcaacagttc agaaataggc
     3781 ataacg
//
LOCUS       DAWBGR010000204         3761 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5695_length_3761_cov_4.439557, whole genome shotgun sequence.
ACCESSION   DAWBGR010000204 DAWBGR010000000
VERSION     DAWBGR010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5695_length_3761_cov_4.439557"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          872 a          964 c         1080 g          845 t
ORIGIN      
        1 tgcaagcaga tggcgtgcaa agtcgccaga cgctctttgt gcggtattgc ctttacaggt
       61 ttgtttttcc tgcgaataac agaaatgtat caaatgtcgg cttgcaattg ctgggaaaat
      121 agggtataat agaaaagcac tgcacagaaa tgtgcgaatc tgttgggaac aaggagagat
      181 tctatgaaac caaaaaaggc gggcagtgtc agacggctgc tgccctatat gaaagggtat
      241 tggaaagagt gcattctcgg accgatattc aagctgctgg aggcgatctt tgaactgatc
      301 gtgccgctgg taactgcgaa aatgatcgac gtgggtatcg ccaatcggga taccggatac
      361 atctggaaaa tgggcggggc aatgttcctg ctggcggcgt gcggactggc attcgctttg
      421 atttgccagt attacgcttc caagtgtgcc tacggcttcg gcacggcact gcggcgggca
      481 ctgtaccgcc atgtgaacac cctttcgcac agtgcggtag accgtatcgg tacggcttcg
      541 ctgatcaccc gaatcaccag cgattccaat acggtgcaaa gcggtctgaa catggtgatt
      601 cgtttagcga cccgctgccc gttcctgatt atcggtgcgg caattatggc aatgcggatc
      661 gacctgaagc tgtccattat tttcctgatc gccattccga tcatcggcgt gctgctgtac
      721 agcgtgacct gctggacgat tccccgctac ggtgagaatc agggcaaact ggacaacatt
      781 gcacggcata cccgtgaaaa tctggacggt gtccgtgtta ttcgggcgtt ctccagacag
      841 gacgaggaaa tcgaatccta ccggaatgac tgcgatgtat tcgccaagcg ttccattgcc
      901 gtgggcagag tgggtgcgat tctgaatccg gcttctttct tcattatgaa tatgggcatt
      961 gtggcagtgc tgtggttcgg cggcattcgc gtggacaccg gtcatctgac acagggtgaa
     1021 ctgactgcat ttgtcaacta catgacacag attgccctgt ccatggtacg tatggcggaa
     1081 ctgttgatct cctttaataa ggcggcggct tctgccaagc gaatttccga cgtgctggca
     1141 gaggagccgg agatcacaga cggcgaaaag cctctgacgg tacaggaaca ggctccggtg
     1201 ctggcattcg atcacgtgac atttgcctat ccggacggcg gtgaggctgc cattcatgac
     1261 atcagcttta gcctgcgggc aggagaaacg ctgggcatta tcggcggtac cggcagcggt
     1321 aagagtacca ttgccaacct gatcccgcgg ttctacgaca ccactgaggg aacggtgcga
     1381 atctacggcg aagatgtccg cagctacaca ctggattccc tccggcagat aatcggcgtg
     1441 gtgccacaga aggcatctct ggtcagcggt acgatcgcgg aaaaccttcg ctggggagat
     1501 gcggcggcag atgacgcaga actggaacat gcctgcaaaa ttgcacaggc gtgggaattc
     1561 gtcagccaga cctccagggg actggaaacg aaggtgacac agggcggacg cagcctgtcc
     1621 ggcggacaga agcagcgtct gaccattgcc cgtgcactgg taggacaccc gaagctgctg
     1681 attctggacg acagtatgag tgcactggac tatgccaccg acttggaact gcggcggcag
     1741 ctggcggcgg aaatgggcga tgtgacaaag atcatgatct cccagcgtgc cacttccatt
     1801 cagcatgctg accacatttt ggtcatggac gacggaaagt gtgtgggcta tggcacacat
     1861 gacgaattgc tggtacagtg tccggtctat gcggatattt accacacaca gatgcagtga
     1921 ggagggaaag tttatgctga cagtcattcg gcgaatttta tcgtatacca agccgtgccg
     1981 caaggaactg ctgggaatgc tgctctttgc gttgatcggc acaggacttt ccctgtttgt
     2041 gccgatcctt attggtaagg cagtggactg cgtggttgct gtacacgagg tagactttcc
     2101 acagctgtgg aaaatcatca tcgttctggc actgaccatt gccgtcagtg ccctgttcca
     2161 gtggctcatg tccctctgta ccaaccgtct ggcggcacac accattcgcg acctgcggtg
     2221 cgacctgttc agccacttgc agcatgtccc gctgcggtac atcgacggac aggcgagagg
     2281 ggatctgatc ggcagagccg tttcggatat ggagatcatt tctgacggtt tcctacaggc
     2341 gttcacccag tttgtcactg gtattttcac cattctgggt acgctgatct ttatgctgac
     2401 gatcaacgtc cgcattacca ttctggtagt cattctcacg ccgatctccc tgctggttgc
     2461 ggcgaaaatt acacagatgt cccgttcctc ctatctgaaa tcctccgatg cccgcggagc
     2521 actgggcggt ctggtggagg agatgatcgg ctcccagaaa gtggtcaagg cgtttaccta
     2581 tgaaaagcgt gcggaagaac ggtttgacgt atacaatact gaattgcagc agaccggagc
     2641 aaaggcaaca tttttcggtt ctatcaccaa tccggtgaca cgttttgtca acgctctgat
     2701 ctatgccgct gtgggtgtct gcggagcact ggcagctacc ggcaatgtgc cgctgatcgg
     2761 tgtgatcacc gttggtcagc tggcaagctt cctcacctat gccaaccagt acaccaagcc
     2821 gttcaacgag atcagcggcg tggtggcaga actgcaaaac gccgtggctt ctgcaaagcg
     2881 tgtgtttgca gtcattgacg aaccggcgga gtcggacgac agccacctgc cggaactgca
     2941 aaactgcaac ggcaccatgc agctttccca tgtgaaattt tcctatgtgc cagaccgtcc
     3001 gctgattacg gatttcaatc tggacgtaca ctgcggacag cgaatcgcca ttgtagggcc
     3061 taccggctgc ggcaagacta cactaatcaa tctgctgttg cggttctatg acgtagacgg
     3121 cggcgaaatt tccgtggcag ggcagaacat tgccggtgtc acacgggaca gcctgcgtgc
     3181 ctgctatggc atggtgttgc aggaaacgtg gctgttcacg ggaagtattg cggaaaatat
     3241 cgcctacagc cgaccggacg cgacacggga agaaatcgtg gctgcggcga aagctgccta
     3301 tgcagacggc tttatccgcc ggctgcccaa gggatatgac acggtgctga cagaggacgg
     3361 cagcggcttg tcacagggac agaagcagtt gctctgcatt gcccgcatca tgctgacaga
     3421 cccgccgttc ctgattctgg acgaagccac cagcagcatt gacctgcgga cggagcagcg
     3481 gatccagaag gcatttgaaa agctgatggc aggcaagacc agctttatca ttgctcaccg
     3541 tctttccacg atcaaaagtg cagactggat tctggtgatg aatcagggaa atgtgctgga
     3601 acagggaacg cacgattcgc tgatggcaca gaagggcttc tacgcaaacc tgtatcagag
     3661 tcagtttgca gacagcgaaa ctacagaata gcagcgtaaa ttacactatg gcaataccgc
     3721 acaaagagcg tctggcgact ttgcacgcca tctgcttgca a
//
LOCUS       DAWBGR010000205         3744 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5732_length_3744_cov_4.557875, whole genome shotgun sequence.
ACCESSION   DAWBGR010000205 DAWBGR010000000
VERSION     DAWBGR010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5732_length_3744_cov_4.557875"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          969 a          960 c         1010 g          805 t
ORIGIN      
        1 tgcaagcaga ttgcgtacaa agccgtcagg cgggctttat tcagcgtttc cttagagata
       61 tgtgaggaac tgtgatgcca gaaccaccag taccagtgcg atcggatagg ttgcagcgta
      121 agcacttgca acgttcggtg tgccgctggt attgatgaga gcacccagag ccggtgtgct
      181 ggtcataccg ccggtgatag aaccgaggtt gttgaacagg ctcagcttca gcaccttctt
      241 cgcgaggaag aagccgatga acagcggaat gatcgtcatc agtgcacccc acaggaacag
      301 cagcggaccg tacttttcca gtgtctgtac gaatccggca ccgccgtcta cgccggcacc
      361 gatgaggaac agaaccagtc cccattcctg aaagacttcc agcagatgct tgttgacctt
      421 catgctcagc ttgccgattc tgccgaagtg tccgaaaatc agacccatga tcagcgggcc
      481 gccggtagtg cccagattaa aggaacctgc acccagcgga atgttgatgc agcccagaat
      541 ggcaccagtc agaacggtca gggcaaatgc acccagaccg aatttgtcca tttcaaacag
      601 ctttttctgc ttcagcggac tttcttcgcc agtgtctaca gaagtcagct tggcacgttc
      661 ttcgtccatg ttggctttca gtaccttcgg tacgatctgc acgaacagaa caacgccgat
      721 aacgccgaac ggatatgcca cggcatgccc tacggcgatt tccttgaaca ccggagaact
      781 ttccccgatc gcttcctgtg ctgcggcaaa tgccggcgta ctggtcagtg caccggacag
      841 cagaccaacg ctcatggcag aattcatgtc cggcaccagc agtgtgatca gagcacagac
      901 acctgcaccg gcagcaatga tgatcgcacc cagcagaacg taggacttgg cgtttttctt
      961 cagattcttg aagaatgtcg gaccggcgat cagaccaacg gaagtgacga acaggatcag
     1021 accaaagctc tggatcaact tgaaataaaa gttgacagcg tcctgtgcct ctacgtcagc
     1081 gatccacacg gcaataccgt tgtcgccgaa tgccacgccc ttgaagtggc caacgaccag
     1141 agccaccaga aagatggcag cggttcccag cgaaacgccc ttgatgttga tcttgcccag
     1201 cagatagcct acgacaatga tcgcaaagac aaagaacagg accatgacaa tggcggacat
     1261 gctgaaaata tgcgtgcctg ccacttcgaa cagttttaga ctcatgaaaa aacctctctc
     1321 cttatacatg aagctgggag aaaccgacag gctctcgttg ggatgccagt ttctccgcga
     1381 taaacacctg tgtgccgtat aaaaacaagc ggaacacaac atactttatt attataactg
     1441 atttcagttt ttttgtcaat aatgccgaac gattttctga aaattgtacg tttccaatga
     1501 ttttccaccc taaaagtgtc gattgactgg ggaaaagcac aaaaccagat gtaacagttt
     1561 gaaaaaatta gtggatttcc aattgcacag tacgaattcc tcaccgaatt tttggaattg
     1621 gaggaaattt ttgtgagtga tgtgaatttg cacaaagata catctccgga ttgacagaaa
     1681 attataaata tgatataata aaagcagttg aaaattctgc aattgcagaa aaaggaggtg
     1741 tcggtaaatg cggcacaatt ggatcacccc caggatgggg ctttttttgg cggcggcaat
     1801 gctgatgtca tcgctttcgg cgttgccgcc ggtgtctgcg gcggaaactg ccatgacaga
     1861 tgatctgctt tcggtgcagg ggattccggt ggaacacacg ccggaaattg ccctttacgg
     1921 ggcaggggac acgtccccgg acgaacaggc agtgtatccg gaggacattt acagttccac
     1981 aatgggctat gacgttctga acgatgcaca aaaaagccta taccgcaaga tcaaggcggc
     2041 ggcacacgca ttttataccg gaactgcggc ggcagaagga gtgtcttacg gttctgatga
     2101 ggaaaagtta ccctgctttg ccatagtcag caatacggat tcttctctct ccaacgagga
     2161 taccgtcaag gtcatttcca tgttccgcaa tgacaatccc atgtattttt ttgtggggaa
     2221 caactacttg tacagcatgg actacgattc tgaaacggaa gaaaactatg ttggtgcggt
     2281 ttatatcgcc tgtgtcgagg aatacaccag cgggacggca cgacaggcgg aacgccgtgc
     2341 actggaaaca cagatcactg cggcacggga acaggtggaa gcacaggata ctgcatgggc
     2401 aaaagccaga gccgccaatg actggctctg caacagcctg acttacgcct atgatgccag
     2461 cggtaatccg gacgattcta tggcatcaca cagcattgta ggggcgtttg atgaacgcta
     2521 ttgtgcggca gtgtgcgagg gctatgccaa ggcgttccag ctgctgatga acgctgccgg
     2581 cgtggcgaat gcctacatta tcgggctggg aaacggcggc gggcatgcgt ggaacatggc
     2641 tcaaatggac gacggctact attattattt tgacgtgacg tggaacgact ccaccagttc
     2701 agacaaatat tttgccgcgg gagaaacgag tttttccaag aatcacacgc ccaacacggc
     2761 agacggcgag agatgggact atctctatga cctgccggac gtgccggagg atgacggcac
     2821 tgatgaaacg ggtactgttc tgacagaggg agatttcact taccagctgt acacagacca
     2881 cgcggttctg acggcgtaca ctggcgagga cgtatctgtt tccgtgccgg aggaggcaga
     2941 cgggctgccg gttacagcga tcaaaggggc atttgccgga aacgctgccg tacaactggt
     3001 ggacctgccg aaaaccgtga ctgcggtttc ctacggcacc ggtggcatcg gggcatttga
     3061 aaactgtacc gctttgcagg cggtttccat gattttgact tcccgcgtgg aataccactc
     3121 gttccggaac tgttctgcgt tgcagactgc cacgctgccg cagacagtta cccttgtggg
     3181 agcaggtgct tttgcggcgt gtaccagttt gaacacgctg cgtgtttatt ccagtgactg
     3241 cacctttggg gcagcatcgg cagttcctgc ggaaacggtg ctctacggct atgcgggttc
     3301 taccgcacag gcttacgcga aaaagtatga ccggacattt ctgttgttcg ggactgtgac
     3361 gactgccgca accacagctg caactgaaac gacaacgacc accacacaga cgacaaccac
     3421 gacccgagcg acaacacaga ctacctctga cagcaccact gcaaccacga cagcaacaac
     3481 tgtcactgcc ggagagattc tgctgccgga ggtgggggac tgtaacaatg acggcgtgtg
     3541 cagggtagat gatctggtgc tgctgaatca gtacctgttg ggcagcgtac agggcagcga
     3601 agtgcagcga cctgcgatgg actgtaacgc agacggcaca gtggacagca gggacagcca
     3661 gctgctggcg atgttcctca tgcagctgat cccacggctg ccggcataac aacggatacg
     3721 aggcaatacc gcacaaagag cgtc
//
LOCUS       DAWBGR010000206         3720 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5789_length_3720_cov_5.201910, whole genome shotgun sequence.
ACCESSION   DAWBGR010000206 DAWBGR010000000
VERSION     DAWBGR010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5789_length_3720_cov_5.201910"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          959 a          910 c          931 g          920 t
ORIGIN      
        1 atgaaagatg ataaacagat aaacttgcaa acgccgtatc aaataaagtt gaccgtttgc
       61 acaagtaacg ccctagaaaa acaacactgc aaacaactag cggcgttggc gagccccagg
      121 cggacagcga ggtacgagcg aattccgccg gacggtttcg acgcgactgc cctagaagtt
      181 acgctttgcg taggaacccc tccaccagct tcgctggtcc ccctcgtact gagcggtccc
      241 ctctgccctg cgggcatctc cccaccccgt ggggagtcac ccctttcagg ggaggctata
      301 agctgatata attaaagctg gtgaaagagt tcgatataga actcctttac cagcttttct
      361 gtttccctta tgggtaggtt tgtagctgca attttctgcg ggacacagtg gtcaaagtca
      421 cagattctgc aaagttgcgg taaaatattg acaaccccgc tgaaatgggg tacaatagaa
      481 gaaagcgttt tttcgggagc attttccgga taaaggcaac accgcacaag ctttgtgcgg
      541 tgttgcctaa aaaattactt tgctgcaaaa aaggagatta ttgtatgaat attgctgttg
      601 cccagtccgg cggaccgacc tgtgccatta acgcttcact ggtaggcgtt tttcgggaat
      661 ccctcaagga acctgcaatt gatgcgattt ttggctctgt caacggtgta gagggcatta
      721 ttcaggatca cctcattgat ctgaaaacca tgatccgcac caatgaagat atggaactgc
      781 tccggcagac cccttccacc gtgctgggtt cctgccggta taaactgccg gattttcacg
      841 aggacagcag tgtctacgaa aagatcgcag ctgtgctgaa acgccgcaag atcggtgcgt
      901 ttttctacat cggcgggaac gattccatgg acaccgtaca gaaactttcc gcctatttca
      961 aggaaatcga tctggacgta aaagtcattg gcattcccaa gacgatcgat aacgatttgt
     1021 gcatcaccga ccacactccc ggcttcggct ctgcggcaaa atatgttgcc actaccatgc
     1081 aggagatcat ccgcgacagc tctgtataca gtattccctc tgtgacgatt gtcgagatca
     1141 tgggacggca tgcgggctgg ctcactgctt ccagtgctgt gctccacgcc tacggcgaac
     1201 cggctccgca tctggtctac gtgccggaaa cccatttctc cgctgagcgt tttctccacg
     1261 acgtggagca ggaaatggca aaacataagg cggtgatcgt ggcagtttcc gagggcgttt
     1321 ccgtgccgga gggggtacag tccggtctgg tggacaactt tggacacaag tatctgtccg
     1381 gtatcggcaa gtatctggag cagctggtgc gggatacgtt cggctgtaag gtgcgttcga
     1441 tagagctgaa cgtcatgcag cgttgctcct ctcacctgag ttccaagacg gacattgacg
     1501 aggcagaaca gatcggtgca gccggcgtgc agactgccct ccgcggcgaa accggacggg
     1561 tcatgatctt ccgccggatt cacaacaagc cctataccat tactatggag ccggcagatg
     1621 cgtcccagat cgccaatcgg gaaaagttcc tgcccagaga gttcctcacg ccggcaggaa
     1681 acaacatcag tgacgaagca atggcatatt tcctgccgct gatccagggc gagctggatc
     1741 tggtaatgcg gggcggcatt ccggtacact tcaccattca ggagtccgtg ttgaagtaaa
     1801 cgtaattttt tcaggcgaat cgtataaagg caaaaccgcg gcagtgtttc acgtgaaaca
     1861 ctgccgcggt ttttgatatt acggagacaa tgcatcaatc ctttgctgca tcggtatcat
     1921 ctgatttttc tgttctgttc tgatacttct gataccaggc actctgctct gcctggctca
     1981 tgttctccca ctcttcctcc cgcttttcaa ttttctcaac ggtgtatgcg tccatgccgt
     2041 agcactcatt tccgtccata tcctgataaa ccgcaatatc gtcttccaag ccgtgataga
     2101 tatactcgtg acgcatcagg ttgcgaaagg tgatataatc cactaaatgt tgattcatgg
     2161 tctgcatgct gccgtagtgc agagagagtg tttctacgat ttcagcatag tccgttttcc
     2221 ctctggaaaa atacaattct tcgagaatgg cagcaagcac attgtcgttg aattgcaggg
     2281 tgactgcgtc cttgtctgcc ttgaccagcg aaacgtcgaa atcccgcaga atcaccgcaa
     2341 gagcagcaat cgtttgtccg gaaaaccgat cctggctctg gatcaggctg tctgtgctga
     2401 cctcaaagta atccgccagt ttcttcaaaa taatcgcaga cggaacgctt ttcttatcga
     2461 tataattgcg aatggacttg gggtcaacca aaatttcagc cgccagcttg ttgatggaga
     2521 tgttccgctt acccatgagc ctttgcagtg tgtccgaaaa gttctttttc cacgcagttt
     2581 cgtcaatttc atagttattc atcatgaaaa ttcctccttt gaaaattatc ctgtagaaaa
     2641 ctccttgcta tctcgatttc cctgtgctaa aataagggta tgactaaaat cacagcaagt
     2701 gcagagaaaa ttccccagtt cagtgcttag agtataccgc atttgtttca ctttgtcaag
     2761 agaaaatcac aaaaaatttt tttggcgaat gctttgggaa ataccgcaca aagattgtgc
     2821 ggtattgcct tacatgcaaa cagcttccgg caaatatcaa agaaaggatc ccaacttcgg
     2881 agcaatcgaa cgaggataac agcgtattca ggaaacattg aatacgctcc aggtgacaga
     2941 gaataatgaa aggtacattt aaaagcggct taaccgcagc agatttgaaa acaatgggca
     3001 tcaagctttc cccgccgcaa tttgacagca gcctgcactt tgacgtttct gtcactggtg
     3061 acagggtgga ggcgttacag aatcgggaag ttttctgcaa tgtggtaaac ttcaacctga
     3121 gagccacggg gaactttatt ccgcggctgg aatatgcaga actttgcagc cccatgtacg
     3181 cgatccagaa aaagatgcag cctgccgact tcaaggttcg tgtttttggc ttcgttcggg
     3241 agaacagaac agcggtcgtc aactggcagc tggaagatgt tgcaacagac atcaagaaga
     3301 tctggaaagg cattgtccgg cagattcagc tggtgcgtgc tgtgaatgac gtgatgcctg
     3361 atgcagacaa aatcctgcgt gcattcaaag tcaaagaggt atctcaggat tatactggca
     3421 caagggtgct tctaggcgga gagttggcag gaatccagcc gagcattgca aacatgacga
     3481 atgtgagcga atttgctcag acacagaacg gtgacatcgt gatgtatttc agcggcaagt
     3541 atgctacatt cacattggta aacggggcta aggttacttt cgtgctggag aatcctgcaa
     3601 cggggatcaa gagcgaacag gaagaactgt atcagggtat ctttttcaag ggtgcatatc
     3661 acaagcggcg ggcggcgggg cgccgctgac cctacgcccc aatttcaact gtaaaaaggg
//
LOCUS       DAWBGR010000207         3711 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5805_length_3711_cov_4.061543, whole genome shotgun sequence.
ACCESSION   DAWBGR010000207 DAWBGR010000000
VERSION     DAWBGR010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5805_length_3711_cov_4.061543"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1276..1285
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1072 a          851 c          892 g          886 t
ORIGIN      
        1 tgagtatggt ataatgtata agagattttg ataagtttcg attttcatat caaaattagt
       61 aaaatgaaaa ggaattttcc tattatgaaa agaattgcag tcgttctttt gtcatttgtc
      121 atgcttgtat catcaactgc ctgcaaaagc aatacaagta caactggcaa tcatgaatcc
      181 gcagcagaga atctacaaga ttctgaaaag atacaactca ccaatcagat gacaccaact
      241 gaactgaaat ctgtgagcaa acatgaactt acacttcttg atgacgcttc tgaagcacaa
      301 acagaatctg ctgtaacatc agaacataaa gtttgtgttg agtattatgc agcagatgaa
      361 cttcgcgaaa ctcttgagaa taatcgcagc aatatgacag aagaagaatg tcaaaaattg
      421 gagcaattga taaatggcag tgagcaatta cctttaaaat atattcttgt agatgatgtt
      481 ttgctgttat atcccgttcc aaacagtgat cccaattatg acggcggcac aattgacttt
      541 gcactcagca atgatggtgc acaagaaaat aagtcctttg ctaattttga agaatatctg
      601 gattggatta gggaagacga aaagcagtat gattattctg atgcggaaat tgagaatgac
      661 atacttcgta tgaggattgc caatgaggca ttgcaaaccg gagaatatga ggtgcttccg
      721 gcgggaagca ttgatgcaac agatcagtcc ctgtatgcca gcaatcaaaa tgcagattat
      781 cgaaacgtat gggaatatga ccgcgatgca gtagagaaaa tcaaggattc tattgacgaa
      841 atcaacattt atgatgagga attagatgtt gattttttgg tgcatgtcac tctaccgccg
      901 gactatgata acagcaaaac atatcccgta ttttttctga cagacggtgt atggcgattt
      961 ggaaattgta cagaactgag aagcgtcatg gaaaatggtg aagcagctcc tgttatactt
     1021 gtcagtctgg gatatgatta caacattgac ggcactgaca acgacaaccg catcacctat
     1081 tttattcaaa aaggcagtat gctgcttgac tttatcacgg ataatctaat gccgtatctt
     1141 ggagaaaatt attctattga ttatacgaat tcaacactgt atggacattc caacggcggc
     1201 gtttttacac atgaagcatt atttcagtct gatttatatg aaaatcagcc gtttgggaat
     1261 tacattattg gaagtnnnnn nnnnnctagg tgttgatccc gaagactaca gcaatgatta
     1321 cggctatttt gacaggaatg atacacttga taaaaaggtt tttctgtgtg gcggaacgtt
     1381 ggaagactct gactacagcg aatactataa cggtcatgat actacactag aaggtttgga
     1441 aaagctgaat cagcggctgg aatctcataa tgcaaatgtc acctatcaat tatatcacac
     1501 acatcactac cagtacattc cggaaatgct gacagcatat ttaaagcaaa catatccagt
     1561 aacttagatt tcatattttc atactgatta tttacgaaaa gaaaggagcg actccaatcg
     1621 ctcctttttc attttcaaaa cctttttgac ttagaattta caattgtgat acaatgcgaa
     1681 atacctgcat atagacaacc gccaactttt ttggtatact atatgataag caaaccaaaa
     1741 ccacacacag aaaggacagc atatgacaga aacatttgtg ttggaatccc accatgaccg
     1801 cctgctgctg catgggctgg tggttcgtcc ggagcaggaa ccctgtcggg gcattctaca
     1861 ggtggcacac ggcatgtgcg agcacaagga acgctacctg ccgttcctac aggaaatggc
     1921 agaccacggc tatctctgca tcattcacga ccaccgcgga cacggtgaaa gcgtccgcca
     1981 gccggaagat ctggggtact tttatccgga cggcggcacg gcgatcgttt ccgacctcta
     2041 tcaggtgaca cgttggatac gggaacagta tccggaactg ccgttgtatc tgttcgggca
     2101 cagcatgggt tcgctggtgg tgcggtgcta tctgaaacgg tatcccgatg tgccggacgg
     2161 tgtgttcgtc tgcggctctc ccagtgccgt ttttggcatg gggctgggcg aacgggtgcg
     2221 ggcactgctc gccaggaaaa agggtgcaca gtatcacagc ccgattctgg cagaagcact
     2281 gttcggcgga ttccagaaac cgtttcggga agaagacaga cctaacgcat ggatctgtag
     2341 tgaccccgcc gtggtagatg catacaacgc agatccgctt tgcagcttca acttttccct
     2401 gaacggctac gaagcattgc tctatctgct gcggacagtc tacagcacag aggactggcg
     2461 ggtgacacag ccggaactgc cgatccggtt tctctccggt gcagaggatc cctgccgcga
     2521 cagcgaccag aaattccagc aggcagcgga actgctccgc agtgtgggat accaaaacgt
     2581 gaccgccaga ctgtttccgg cgatgcggca cgagattctc aacgaaccgc aaaaagaact
     2641 ggtctatcag gacattctga aaaccataca gatatgggag gaaaactaat gaacattcca
     2701 aaggaaatac aggaaagccg cattgcttgg gcggcaaatg atgccaagcg ggacagcggc
     2761 ttgcaggagc cgcagggcat agaaaaattc tgcgatctgg actacgccgg tgacggcatc
     2821 acggagcact tgctggacgt ataccgtccg gtgcaaaacg gcgaaaatct gccggtgctg
     2881 atcagcattc acggcggcgg ctggttctat ggggacaagg aactgtaccg gttctactgc
     2941 atgcacatgg cggaactggg tttcgtggtg gtaaactgca actatcgcct tgctccggag
     3001 cacccctatc cggctgccat tgccgatgtg tgtcaggtga ttcgctggac actggcacac
     3061 cggaagcagt acacgtccaa cccccactgg tttctggtag gcgattccgc cggtgcccag
     3121 ctggtcagcc agtactgcat tctggcagga aacgccgcct atcgggaaca gctggacttt
     3181 gccacctatg accagctgcc ggacggcact gctctgaact gcggcatcta tgacatgtca
     3241 ctggaaaata tacacaaaaa catttacaca caggaagccg ctccggaaca gcagaagctg
     3301 tttgaacaag ttctcaccta tatgaacagc agctttccgc cctcgtatct catggcaagt
     3361 gtcaacgacg ggctgcgtcc ccgcacaacg ccgatgcggc agcggctgga agcatgcggg
     3421 ataccgttca cctttgcgga atacggcgag ggcgtaccgt cagacagcca cgtattccac
     3481 ctgaacttgt acagtgaaaa cggcaaagcc tgcaatcagg cagaggcgga tttcttccac
     3541 catctggagc aacaggaaac cagacacgcc tgaacacgca gcaatatcca aagggcaaca
     3601 ctacccaaac caaatgcccc cgatttttcc aaaaagaaag tcgggggcat tctgttattc
     3661 atagggcaat accgcacaaa gattgtgcga cagatgcgtc gtcaaatttt t
//
LOCUS       DAWBGR010000208         3679 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5866_length_3679_cov_4.863135, whole genome shotgun sequence.
ACCESSION   DAWBGR010000208 DAWBGR010000000
VERSION     DAWBGR010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5866_length_3679_cov_4.863135"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          968 a         1071 c          840 g          800 t
ORIGIN      
        1 ctccgctttt tgtaatatac aattatacga attttattta tccgattctt tgttatagaa
       61 aaacataacc actgccatag caatgatgat acagtacccc agaatgctca gccagtcggg
      121 aatctgtgca aacagcaaaa atcccaagcc tgcggcaaaa atgatctggg tgtaatcgta
      181 aacggaaatt tcctttgccg gtgcatagca gtacgcagaa gtgacaccga actgtccgcc
      241 tgccgccgaa agccctgcca taaccagaca caacagctgc ttccacgtca taggctcata
      301 gcagaacagc agatacggca atgtcaccac acaggaaaag gcggagaaga aaaagacgat
      361 atacgcctta ttctccccga tctgccccag acggcgtacc atggtatagg caacgcctgc
      421 cccgaagccg cccagtagtc cgatcaggga cggaaacaaa tccgcattgg caaaggtggg
      481 cttgatgaca cacagtgccc ccacaaatgc ccccagcacg atcagcccct gtttccagtt
      541 cagtttttcc cgcagaaaga tccacgaaca gagaattgca aaaaacgggg acatcttatt
      601 cagaatcgac gcgtccgaca gtgccagatg atcgatcgcg taaaaattgc acaggatccc
      661 cagcgttccc gccgtggcac gcagtaccag aaacggcaga gatccctttt tcggcagaaa
      721 gcagccgtgt tcccgcagaa gcatcgccag tgcgaacaac agtgccacca gattccggaa
      781 aaagcttttc tggataaacg gcagatcgcc ggagagccgc acaaaggcat tcatccatgc
      841 aaaacaaaac gccgacaaaa tgatgcagat catgcccttc tgccggtcag acaaagaacg
      901 ctttgtcatg attttcatct tctttcattg acgtgtttta tggctgacat agccgccggt
      961 gaacttggag gacaggaacg tctgttcccc atacagcccg taatagccgg agagcagata
     1021 gctgacggcg actgccagca gataatacgg agcacccgcc ataccgaaca gttcagatgc
     1081 gatcatcata gaagccagcg ggcagttggt cacgccgcag aacagtgccg ccatgcctac
     1141 tgccgcacag agagagggcg aaattcccag caagtgcccg aacagacagc caaacgttgc
     1201 cccaatgaaa aaggaaggta cgatctcgcc gcccttaaag ccgccgccca atgtcaacgc
     1261 cgtgaagatc atcttcgcca gaaacgcata ccagaccacc tgccctgcca cggcacgccg
     1321 catcacctcg ccgccaatgc ccagatagtc ctgattatgt accagcaacg ccagcaccca
     1381 gagcacaccg cttgccgcca gaatccgcag atatggattt ttgcaatact ttttcaggaa
     1441 gtgttccgtc tggtgcagca gcacacagaa cagaatgctc accgccgcac agcaaacgcc
     1501 cagcagcaaa attttcaatg ccggcaccac ctgaaacgcc ggaatttccc cgatcgtata
     1561 cttcgccagc ggaatgccgc atttgcccgc cagccacgaa gccgtcagag aagcaatggt
     1621 acagggcacc agtgccgcgt accgcatggc accgacacac accacttcca tggcgaaaac
     1681 cggtgccgca atgggcgtac ggaaaattgc cgaaaacgcc gccgacatgc cgcagagcat
     1741 cagcacctgt ttatcccgtt ccgccagacg gaacacccgt cccagcatac tgcccacact
     1801 gccgcccagc tgcaacgccg caccttcccg tccggcactg ccgccgcaga agtgggtcac
     1861 tacggtggaa atgaaaatca gcggtgccat ttgcataggc agttccgttt ctgcccgcag
     1921 cgaagccagc accatattgg tgcccttgtc gttctggtcg tgactgatgc ggtacagcca
     1981 cacgatcagc agaccgccgg cgggaagtgt cagcactgtc caccaatgcc ccgtccggaa
     2041 tgtcgtcacc cactgtatgg catacacaaa agcaatcccg ataaatccca ccagaacgcc
     2101 catcagcaca gagtaaaaca cccaccgcag cagcagccgc aaatgggaaa gaatgtggat
     2161 accgctgtgc agcagccgtc ctttccagtg ggaaagaaaa cgttcccttg aacgcatacc
     2221 attcctcctg acacaaacgc aatcctccgg aaagtcatgc tttccagagg atttccgtcc
     2281 aattttattt gcaagatatt tgcaaggaaa gtgatttcct taaggcaaca ccgcacagag
     2341 cttgtacgta agatgcctta atagttctca gccttgacct gaaaatagga ctgcgggtgc
     2401 tggcagaccg gacagacctc cggagccttt ttgcctacga caatgtgacc gcagttggca
     2461 cattcccaga tgacatcgcc gtccttggaa aagacgcagt cgccctcgat attggcgagc
     2521 agcttgcgat aacgtgcttc gtgttccttc tcgatcgcac ccaccgcctc aaacagtgcc
     2581 gcgatctgtg tgaagccctc tgcctttgcc tgctccgcga actcgacata catatctgtc
     2641 cactcgtagt tttcgccggc agccgcatct ttcaggttct ccgttgtttc cggcatcacg
     2701 ccgccatgca gcagcttgaa ccagatcttc gcgtgttcct tttcattgtt ggcagtttcc
     2761 tcgaacagct tgccgatttg cacataaccg tctttctttg ccttggaagc gtaataatta
     2821 tacttggtga atgcctgtga ttcgccggca aatgccgcca gcaaattttt ttccgtctgt
     2881 gaacctttca gttctgccat gatatgacct ccttttcatc ttggagaaag caaaacaaaa
     2941 ctgttgcata caaacaagtt ttattccgca tttttcctat gtggttcttc ggtggtttgc
     3001 agctgtgccc gctgttccgc cgcctttcga caggcgggac acaatccgga aaagacaatg
     3061 tgatgggatt ccaccagaaa atcctcgcag cctacgatct tgctgcacag gtcgctctga
     3121 tagggcatgt tcacatcgaa cacccgattg cagctgcggc attccagatg atagtggggc
     3181 ttggtgttga aatcgaaaca gtctgccccg tccggaatgg gaatgtgcag gatctcgccc
     3241 tgctctgaca ggatcttcag attccggtag accgttgcct tgctgatcag cgtgttctct
     3301 gcggcgatct cccgaaagat ttcgtctgcc gtagggtgat tgtgcattcg ccgtaccgtt
     3361 tccagcacca gacgacgctg aagcgtattg cgatttttca taaaaatcaa tcctttaagc
     3421 cgagcatata caagatatgc tctaatagta tcagttctta attaacatta tatactatct
     3481 gatttgactt gtcaagagat ttttctaaaa aggcaacaca acaacatcat ttcccctctt
     3541 ttattgtcgc cggtttccgg aagattatac aaaaaagcct gccttgacaa aaagcaagac
     3601 aggctcgata ccacaaatta cgctttacgg caataccgca caaagattgt gcgacagatg
     3661 cgtcgtcaaa tttttcgtc
//
LOCUS       DAWBGR010000209         3677 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5871_length_3677_cov_5.861126, whole genome shotgun sequence.
ACCESSION   DAWBGR010000209 DAWBGR010000000
VERSION     DAWBGR010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5871_length_3677_cov_5.861126"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          914 a          829 c         1018 g          916 t
ORIGIN      
        1 ccccgtgggg agtcacccct ttcaggggag gctatcatga aaattgattt ccgtgcttta
       61 tgtagtattt cccttgcaat tgtcaaataa atatggtata atgaatgcat ggcaacgcgg
      121 tatttgtgga gtgctgccat atctcagaac aaaggagtca gaagaacatg aagatttgga
      181 ttggatgcga ccacgccggt tacgagcgaa agcaggaagt gatcgcactg ctgaaagaac
      241 ggcagttgga agtggaggac tgtggctgtg acggagagag agccgattat ccggtgatcg
      301 ccaaggcagt tggtgccgca gtagcatcgc actccggtga catgggaatt ctgatttgcg
      361 gcacaggcat tggcatgtcg atcgctgcca ataagatccg cggcgttcgt gccgcactct
      421 gtgcagatgc gttttctacc aagtataccc gtctgcacaa tgatgccaat gtgatgtgca
      481 tgggtgcccg cacactgggc agcggtctgg cactggaatt ggtggagatc tttctggata
      541 ccccgtttga gggcggcaga cacgaaaagc gtgtgcatat gatcacagaa ctggaacagg
      601 aatggtaaca caggaggaaa aaattatggc aggaattcat gtagtagatc acccgctggt
      661 gcagcataag atctcactga tgcgggataa aatgacaggc gtaaaggaat ttcgggaact
      721 gacttcggaa acagcaatgc tgatttgcta tgaggcaacc agagatctgc cgatgaagga
      781 aatcacgatc gaaacacatc agggaatcgc aaagaccaag gtcatctccg gcagaaagct
      841 ggcattcgtg ccgattctcc gtgcaggtct gggactggtt gacggcgtga cttcgttgat
      901 tccggcggca aagatcggtc atatcggcat tttctgcgaa ccgggcacac atgatgcagt
      961 gaagtattat tccaagatgc cggacgatat tgcagaacgg gaagttatcg tagtagacgc
     1021 gatgctggca accggtactt ctgcgatcgc agcgatcaag gatctgaagg aaatgggtgt
     1081 caagagcatt aagttcctgt gcattctggc ttgtccggag ggacgcgatg ccctgctggc
     1141 agcacatccg gacgtggaca tctactgcgg cggcgtggac gaaagcctga acgaaaaggg
     1201 ctatatcgtt ccgggtctgg gcgatgccgg cgaccgtatt ttcggcacaa agtaaaaggc
     1261 aataccgcac aaagcccgcc tgacggtttt gtgcggacaa catctgaaat agatttcaaa
     1321 aaaaccgtag cggagagaat cgctctttcc gctgcggttt ttgttgttat ggcaataccg
     1381 cacaaagatt gtgcgacaga tgcgtgcgag aagatacgaa acaggctctg aagggaaatt
     1441 tgcaagcaga tggcgtgcaa agtcgccaga cgctctttgt gcggtattgc cttagataag
     1501 cagacgctta ttcatatttt gcaatgattt ccagagaaac ctcttttcgg gtgcgtctgg
     1561 tgtccattgc acttttctca atgtaacggt gtgcagcttc ttccgacatg ccctgttccg
     1621 tgatcagcag gaactttgca cgggaaacgt accgcagttc ctctaacttg gcacgaagct
     1681 tgttgttttc cttttgcagc cccagcaaac gatggcgaaa ggcacttccc atgcggatcg
     1741 cctgtaacag ttcctgcttg ttcagcggct tgggaaccac acagactccg tagtcactgg
     1801 tgcgattggt catttcctct gccagatctg ccttgcagat cagaatgaca ccggaacatg
     1861 tggactcaca gagtttcact gccagctggt gtccgaattc gtctttcagc ggcgtgttga
     1921 tcaaaatcag gtcaaagtca tcatgaggaa tcatgcgtct tgcttcgctg ccgctggaga
     1981 tgactgcaac ggagccgcag ccacaggctt tcaaaaagct ggtgacactg tccagtcctt
     2041 tttcagagcc ggagatcatt aacgtgcgtt ctgaatacac cgttgtgttt cctcctcttt
     2101 ttcagattgc agttttccgg ctgcttaaaa atatcggtcg aagcagaatg cttccggatc
     2161 ttttgccaga tgataggcat cgacttcttt ctgcattgcc gcggaaaaat cgtggagaat
     2221 tgccttgggc acagtgttac ggacaaattc gctttccagt gccagctgga tagattcttc
     2281 cagggagtgg ggaagtgtgg gaatggcaga attttcattt gctgcattca tgttgtcgga
     2341 cagttcctcg ttgttctgga ttcctgccat gccggcactc agcagcatct ggaatgtgat
     2401 gtacggattg cagtatgcgt cagcggaacg gaattcaatg gagccgtccc gacccggagc
     2461 atacagcagc tgtaccagcg gaatgcggtt ttcctcggac cagttgatgc gggagggtgc
     2521 cgcacgatag ccgaagcggt cgtaggagtt gatcgtgggg ttggagaaca tggtgaattc
     2581 ccgtacccga ttgaggattc cggcaataaa ggctctgccc tccggggtaa gggcttcgtg
     2641 gctgttgccg aaaatgctct tgtcgtcttt cttgatgcac aggatgatct tcagcccgct
     2701 gccgctgcag tccttgaacg gcttcggcat aaacgaagcg tagaagccat tctgtgccgc
     2761 aatggtcttg actacggtct tgtagtgagc catgttgtcg gcagctgtca gcggattgct
     2821 gcatgcgaag tcgatctcgt tttgtccggg gccgtgcttg tggcaggagg gggtagggtt
     2881 cagtcccatt tcctccagcg acaggcagat ctcgcggcgg gtgttttcgc atttgtccag
     2941 cggagccacg tccagatagc cgccctgatc gcagggcttc ttggtaggct tgccctccat
     3001 gtcggtttcg aacaggtaga actcgcagcg ggtgcccatt tcacactgat agccctgatt
     3061 ctgaagggac ttcatggttt cccgcagatt gcggcgaaga tcgccttcgt agggagtgcc
     3121 gtccatgtgg tacagtttac agaagaaacg taccacacga ccggactggg gccgccacgg
     3181 cagaatggac agggtagagg tgtccggttt cagcagcagg ttctggtgcg atgcctccag
     3241 cagtccggtg gcattgaacg gaatgccgtc tgtaatggca cggtgcagtt cccgtggcat
     3301 gatggcaata tttttcatat ttccaaacgt gtcgcaaaag gtcatgcgaa taaattttac
     3361 gtcattttcc tcgatgaagt cggataggtc agtttcagaa aaaaacatat gatacctcct
     3421 gaaagtatac tatttttagt atacaatata tgtttcttga taaagcaatt agaatgtgta
     3481 caatatgtaa aaaaacgaat tccgtattga aaaagataag aaactatgat ataatgtaaa
     3541 tagaataata aaaagctttt tccgaagaac ttaaaaagac atatattgaa agctgaattt
     3601 caaagcttta tgggaggcta tcaatggaaa atctgaaatt taaggatata aggcaatacc
     3661 gcacaaagag cgtctgg
//
LOCUS       DAWBGR010000210         3671 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5880_length_3671_cov_5.145465, whole genome shotgun sequence.
ACCESSION   DAWBGR010000210 DAWBGR010000000
VERSION     DAWBGR010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5880_length_3671_cov_5.145465"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          819 a          962 c         1015 g          875 t
ORIGIN      
        1 acgaaaaatt tgacgacgca tctgtcgcac aatctttgtg cggtattgcc cttgaacaca
       61 cgtaaaaatg tttgggcagc atctgccctt acttctcctc ctccgcgtgg tgagaaccgc
      121 cggaggtgac gatctttcgc cggaaatcgg attctccctg tccgatgacc ggtcgtgcca
      181 gctggtagat gtcggttgcc tgtgattcca gtgccgcaca gcgtcctgcc tccggactgg
      241 tgtgagccag ttccgccagc gagtgggaaa agggcagcag ccgcttgttc ttggaatccc
      301 gcaggatctc ccgtccggtg tcgttgaacg ccagaatccg accgtatgcc ggataattct
      361 ggatgtctgc cgtgcggatg tccagcagaa tgtgcagcag gatccgccgc agccgtgcca
      421 tgggatagcg tttggtgcgg atcgcctgta acagttcttc cagcgaagcg gaacgctttg
      481 ccttgtacag acggtgctcc agtccctgtc cgatctcggc aatgtcgtgg agatcgtctg
      541 catcggtggt acgcagtttg tacagaatga gccgttccag atattccata gaggcgatgt
      601 gtccgttctg ttttgcttcc cgcagggcat gccatgcata agaaggcatc attcgccggc
      661 agtcgccgcc ctccgcaaaa aactgccgga tataggtggc actggcggtg ccgtccggtg
      721 ccggctgact gctgttgtgt ccggctcctc tgcggggaat ggtcatgggc tgcaggggac
      781 tgtccatttc ttcaatggcg ttgaggtagg caatggcaag ggtgttgttg gggctgcgga
      841 gcacggcagc tgtttcttcc ccgtagagct gcccgaccat ctgggagagc acctccggat
      901 aggaatagcc ggaacggaga aaatcgtcca tgacatcggc gtagtctgtc ttgcagacct
      961 gacacgcctg tgccgcctgt gagagcaggt gaatgtcccc gcactcgctg ccgaaagaga
     1021 tctcctgcac attccccaga gccgtcagaa gtgcaacgcc gcccatggcg aagttctccg
     1081 ccggagccag agcatagggc accggcagtt ccagcaccag atcgacaccg ccctcaatgg
     1141 caagacgggc acgggtgaac ttgtccagca gggcagcatc accacgctgg acgaagttgc
     1201 cgctcatgac ggcaacgatc ccctctgcac cgccccggcg aacctgttcc agatgataca
     1261 ggtgtccgtt gtgcagggga ttgtattcac agataattcc actgattttc atgtatgaac
     1321 ctcccgaaaa gctgtagaaa atgagaaaat tgcatgattg gtcggggaat ttgcggaaac
     1381 cccttgcaat ttttgcaaaa aagcttataa tagtatatgg gacactcctg gtgtccgaaa
     1441 aatgatgtca gcggcagagg tctgcttcgg ggacatatcg gaaaagcaag caggtactgc
     1501 cgccggcaga gtttgctttt ccgatgtgtt ccgacaggtg ccgcaaaaat gaaaaaggag
     1561 aaattttcct atgattattc ttgttgtcaa tgcaggaagc tcgtcactga agtatcagct
     1621 catcaatatg gaagatgagt ctgtcatcgc aaagggcaac tgcgaccgta tcggtatcga
     1681 tggtcacctg agccacaaga ccgcttccgg cgtaaaggtg gaaaaggact gcgatttccc
     1741 gacacatgca gaagcatttt cctgtctggt agacgcactg actaccggcg agggtgctgt
     1801 gatctccagc atggacgaaa tttccgcagt tggtcaccgt gtagtacagg gtgcagaagt
     1861 gttcaccaag accactctgg ttaccgatga aatcatcgaa aagatcgacg aactccgcga
     1921 actggctccg gtacataacc acggtcatgc actggctctg tgggcttgcc gcaagatcct
     1981 gccgaacgtg ccgcaggtcg ttgtttttga caccgcattc caccagacca ttccgcagaa
     2041 ggcattcatg tacggcttcc cgtatgagga ttacgagcag ttccatgttc gtaagtatgg
     2101 cttccacggc acttctcacc gctttgtcac tgccaagctg gcagaaatta tgggcaagga
     2161 tctgaaggat ctgaaggtgg tttcctgtca tctgggcaat ggttcttcca ttaccgcagt
     2221 acaggacggc aagtctgtag ataccaccat gggcttcaca ccgctggacg gcgtactgat
     2281 gggtacccgc tgtggtgccg ttgacccgtc tgctgttacc tatgttgcag gcaagcacgg
     2341 cttcggcgtt tccgaaatgg acacctacat gaacaagaag tccggtatgc tgggtatttc
     2401 cggtaagtcc tctgacttcc gcgacatcac cgcagcagca gaagcaggcg acaagcgtgc
     2461 aacactggca aaggatatgc tggtatacga catcaagaag gacatcggtg cctatgccgc
     2521 agctatgaac ggtctggacg ctgtgatctt caccggcggt atcggcgaaa atgcaccgaa
     2581 ttgccgtgag gaagtctgcg agaacatgga atatctgggc atcaagatcg acaaggctgc
     2641 caacgatttc aagggcgagc tgaagaagat ctccacaccg gattccaagg tagaagtctg
     2701 ggtaatcccg acaaacgagg agctgctgat cgctcgcgac acactggcaa tgatccagaa
     2761 gtaagccgtt tatcagttgc tttcaatttt tcaaatactg gaaagcgaag tcttttcgaa
     2821 ggcttcgctt ttgttatacc ggagggaaaa tcaatgtcag atatgagcac ttttgataat
     2881 gccgaactgt tgcagcaggc tctgtctgcc cgcagaaacg cctatgctcc ctattcccat
     2941 tttcaggtgg gagctgtgct ggttactgcg gagggacagt acttttccgg ctgtaatatc
     3001 gaaaacgcgt cctattcgct gacggtgtgt gccgaacgca gtgcgatttt cggggcagtt
     3061 tctgccggat tccgacagtt ccggaaaatc gccattgtag gcggcatgac ggaagaagat
     3121 gcccgtaccc agtcctgtcc gccctgcgga gcctgtttgc aggtgctggc ggaattctgt
     3181 ccgctggaat ttcccgtggt gctggcagac agggaatacc agctgcggga gttcctgccc
     3241 cgtcagtttc ggctgtaact ctagggcaac accgcacaaa gcccgcctga cggctttgca
     3301 cgcaatctgc aagcatctta cgcacaagct ttatgcagtg ccttctttat tttttagact
     3361 tatccttgtc cttgtctgcc ggtcggtagg aagaaccgtc cggacgaatg acaacggtgt
     3421 tgtccagctg tcctttcaga tttccgatga cggaacggcg gtactccagc cggagagcgt
     3481 tctgctcctc ctgttcggca ggagtcagcc cttcttctgt gcgggatttt ttcgccagtt
     3541 cgttgatgcg tgcgattttc ttgtcgtcca tatgtgatgc ctccttagag aaaacatttt
     3601 ccttgtatct tacaatttaa ggcaataccg cacaaagatt gtgcgacaga tgcgtcgtca
     3661 aatttttcgt c
//
LOCUS       DAWBGR010000211         3669 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5884_length_3669_cov_4.503597, whole genome shotgun sequence.
ACCESSION   DAWBGR010000211 DAWBGR010000000
VERSION     DAWBGR010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5884_length_3669_cov_4.503597"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    350..359
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1023 a         1016 c          928 g          692 t
ORIGIN      
        1 ataatgtatt tattcactat aacaacatgt gaagtgaaac acagaaccca caaaaatcgg
       61 aaagaaggaa tgaaatcaga tgaaattcaa aaaactcatg gcggcagtca ccaccggtgt
      121 gcttgctgtc accagtctgt ccctttcggg actgatcggc tccgcagagg tgcaggagct
      181 gatgaacgac acattcgaaa gcggctacgg agcatggaaa ggtgtaggct cctcgctgtc
      241 cctgtccacg gagcaagccc acaacggcgg cacttccctg tactgctatg accgcaccgc
      301 cagctggggt gctccccgct gctccctgac cggaatcgtg gctgccggan nnnnnnnnnt
      361 acgagatctc tgccagtgcc atgtacgagg gcagcggtca gcagaacatg gcgatcaaaa
      421 tgatctacac cgatgccaac ggcacagacc actacgatca ggttgccgcc gcacaggcaa
      481 ctgcgggaca gtgggtggag atgaagggca actacaccgt tccctccggt gccaccggca
      541 tgatcctcta tgtggagatg ccgggtgcca ataccgacca gacctattat attgacgacg
      601 ttgtcattaa aggcgaaaag acagagatcc agctggacga caaattcgaa tccgacttcg
      661 acaacaactc cacccagaag tggaacggcc gcggcagtgc caaggtagaa ctctccacca
      721 agtacgccca cagcggcacg accagcctgt atgtttccgg cagaacccag ctgtggaacg
      781 gtgcgacccg cagtttgtct gacatcatgg aagccggcgg ctactacaaa gtcggcacct
      841 acgtgctcta tgacggcgac cagtactccg acacccagaa attttccatc aacctgcaat
      901 acgacctgaa cggcaaggaa aactactaca ccattgccac cgaaactgca aacaagggcg
      961 aatggaaata cgtgggcagc gaattcaccg tgccggaggg tgcgaccaac ttctatacct
     1021 atgtacagac cggctacacc agtgccccca aggaacagga tctcatgaac ttctacatgg
     1081 acgatgcagt cggcgaacac ctgccggatc ctgccattca ggacgacatt gcttccctga
     1141 aagatgccta ttccgactac tttaagatcg gctgttcctg taccggttcg gaattcgcac
     1201 agggtgccac caaagacctc atcaaaaagc actacaacag cctgacactg ggcaacgaac
     1261 tgaagccgga cagcgtgctg gatcaggcac tgtcccagaa gtacgtggca gaaaccggcg
     1321 atgacaccat gccccagatc tctctgaacg aagcagacaa gattctgaaa ttcgccggcg
     1381 aaaacaaaat tccggtacgc ggtcatgtgc tggtatggca cagccagaca ccggactggt
     1441 tcttcaagga aaacttcgat cccaacgggg catgggtttc caaggacaag atgaccaagc
     1501 gtctggaaaa ctacatcaag accgtcatgg aaacgctgaa aaaggattac cccgatgtgg
     1561 aattctacgc atgggacgtg gtgaatgaag ccgcttccga tgccggcacc attcgcgatg
     1621 caggttccaa caacgaggtc gacggacagt ctgcatgggt caaagtctat ggcgaccagt
     1681 cctatatccc gctggcgttc gaattcgcca agaagtacgc accggcaggc tgcaagctgt
     1741 tctacaatga ctacaacgag tactctccca acaagcaggc gtacatcatc agcgacattc
     1801 tgaaaccgct ggtggaaaag aacctgatcg acggcgtggg catgcagtcc cacatctcca
     1861 tgagttatcc caccattgat ctgtacaagt ccgcgatgca gcagtatgca gacctgggac
     1921 tggaagtaca ggtcaccgag ctggacgttt ctgagaagtc caacgaatac gccaaccagc
     1981 tggcactggc acagcggtat caggacgtgt tcaagatgta caaggaaatg aaggattccg
     2041 gcgtgaacct gtccgctgtt gtcctctggg gcatcaccga ctccaccagc tggatcggcg
     2101 gctatcccct gctctttgac aaggactatc aggcaaagcc gtcctattac gccgtgatcg
     2161 acactgactc cgaagtggaa aaactccaga ccatgaccgc ataccgctat gacggcacag
     2221 atgccgatct ggaacgggca ttggaaatcg gtacggcaca gtatctcagc accaaaaccg
     2281 gcagtaccaa cacttatttc aaggcagcct ggtctgacga aaacatgatc gttcgcatct
     2341 acaatccggc agtttcagca gatggcaaaa attcctatgt tgcaatttac ggaaaccaag
     2401 tggattctat gggaggcttc ccgattgcca gccaaaccat tacttccgac atggaatata
     2461 ttgacctgaa atggaaatcc aacagtaatg ctggttggga accgaaaaac ggcagtaccg
     2521 tgcacttaga tgtattcaac aatgatgctg catggaattg cattgatctg gcaaaaagct
     2581 tttttgacat gatgaatgaa agtgaaccga ctggcttcgg tgcattgccc aagtgcggta
     2641 ttgtcaccct cgcagaacag cccaagtatg ccgaggcaac caagactgaa acacccatta
     2701 ccattgacgg ggacatcgat gccgcatggg cagatgccaa caccattgat gtcaacacct
     2761 actccatggg caacggtgcg accgccgttt ccaaaatgct ctgggacgag aattacttct
     2821 atgtgctgac cgaagtcacc gatcccgtac tgagcgtggc aagtgccaac gcctacgagc
     2881 aggacaccgt ggaagtattc ttcgacgaga acaatcacaa gacttcctct tacgaaagcg
     2941 acgacattca gtgccgcatc aactacgaga acgacaagac cgttaccgac ggacgttcca
     3001 ctgacgcatt cctctccggc acgaaaaaga ccgacaaggg ctatatcgtc gaggtggcaa
     3061 ttccgtacac tatcggttcg ttccacgctg accagatcgt aggctttgac gtacaggtca
     3121 acgatgacgg caccggcgac ggcaagcgta ccagcatggc caactggaac gacctcaccg
     3181 gtcagggcta catcaacacc agcggtttcg gtgtactgaa actggtgggt gacggcacag
     3241 ttaccacaga caccactgta acgaccacca ccactggtac aacttccacc acaacagaaa
     3301 ctgtcacctc tgccaccacc tccgacagca gcacaaacac ttcggaaagc ggtgaaagcg
     3361 gactgacact gtacggcgat gtcaacatgg acagccgtgt agacattacc gatgcggttc
     3421 tgctgaacaa ggcagtggca aacgttgtca cactgggaag cacccagaag aagaatgcgg
     3481 actgcgatgc cgacagcgag atcagcggca atgatgctgt cgttctgctg aagttcctgg
     3541 tatccattat ccacaccctg cccagtgcag aataagcgta cactgacaag cgaacacgat
     3601 gtaacggtta aaaatgagga taagcctccc ttgaaaaggg agggggacca gcgaagctgg
     3661 tggagggat
//
LOCUS       DAWBGR010000212         3641 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_5940_length_3641_cov_4.090351, whole genome shotgun sequence.
ACCESSION   DAWBGR010000212 DAWBGR010000000
VERSION     DAWBGR010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5940_length_3641_cov_4.090351"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          704 a         1098 c          947 g          892 t
ORIGIN      
        1 tttgcaagca gatggcgtgc aaagtcgcca gacgctcttt gtgcggtatt gccttctgtt
       61 attgtacctt tgccgtgatc tcaccctctg ccagctgcac ccgcagcaca tcgccggact
      121 gcacctgtgc ggcactgcgt accagttctt cgccgtggta cgccagtgca tagccacgcc
      181 ccagaatctt catggggctg agcatgtcca gacggttggt ctgatgcacc agctgttcct
      241 gacattgctg ccagatctgc cggatacccg cttccagccg cttctgcaaa tggcagagtt
      301 cctgctgtgc cagttcctgc cggtgtgccg gtgactgtaa tttcagccgg tcttgcagcc
      361 gttccagttc ctgtgctttc tgccggaact gctgctgcat ggcgttttcc agccggcggg
      421 agcagtcctg tacctgctgt agcatctgcc gcacatcagg gaccgccagt tctgccgctg
      481 ccgacggagt cggtgcccgc aggtctgccg ccgcatctgc cagtgtccag tcggtttcat
      541 gccccacggc agaaaccacc ggcacggtac agttgtaaat cgccatgacc acctgctccg
      601 tattgaacgc ctccagcgtt tccgaagcac cgccgccccg tccgacgatg aggacatcgc
      661 agctgtcccg ttccgccgcc gcaatggcac ggcagacaga agccgccgcc gcatcaccct
      721 gcacctgtgc tggatagacc agcaggtgtc cgatcggata ccgcctgccg ataacgtttt
      781 tcacgtcctg taaagcggct ccggtatcgg aggtaatgac accgattttt ttgggcattg
      841 ccggcagcgg tcgctttgat gccgcatcga acacgcccat tgccgtcagc tttttcttga
      901 gttgttccag tgccagagcc tttccgccgg tgccgtccgg ctggagatct gtcacataga
      961 tctgaaaggc accgtcccgt tcatagaggg cagcggaggc ggcggcaatg acgttcatgc
     1021 cctcctgcgg cagaaatttc agccgctggg catggctccg gaacatcact gccttaacac
     1081 agctttccgc gtcacgcaac gtgaaataca ggtgtccgga acgatagtga ttggtgaaat
     1141 tggtgatctc tccccgcacc agaacgcttt gcagggcacg atcctctttc agcttgaacc
     1201 ccacatagcg gttcagctgg gtcacgctta aaattgccac agttttcctc cttgcttccg
     1261 ccatgcacca tagactgcca cacctgcggc attgtcacag gcgaactccg gtgcagcaaa
     1321 ccagcattcc gcattcgccg ccagcagcgg acgaatcagc tggttcgaca tcacgccgcc
     1381 ggcatagagc accggcagct tgccgtgccg ttcctgtgcc gccttagtca tagcgatcac
     1441 agtttccgcc acagtcatca ggcagaaacg ggcaatatct gccggcggtt caccctgctc
     1501 cagctttttc cggcactggt tttccagtcc ggacaggcag cagtctacgc cccgtatcac
     1561 cggtttgatc cggaacggtt tccggctttg ctgggcaagc tgttccagtg ccttgccgca
     1621 gggaaactgc aatcccagca tcagccccac ccgatcgatc gcctgtccgg ctttcagatc
     1681 cagagaggta ccgcaggggg tgatcttcaa ggcagcctgt tcgtccggtt cgcagtacag
     1741 ggaatctgtc gtgccgccgc tgacatggaa cgccagaaac ggtttttcac aggctcccag
     1801 cctgccggca gagtacagtg ccgccagcac atgccccacc tgatgtgagg tttcatacag
     1861 gggtacgccg gtaactgcgg caacggcacg ggctgtgcct acgccagtga gaaaacaggg
     1921 catataagag ccgtccacat tgcggggacg ggtggaaacg ctgacaccgc agaccggatt
     1981 ttccgccaga aacggacgaa gccgttccag cagttccggc agctggcggg tgtgatgaaa
     2041 taccgcatca ctctgccgca gtccggcttc tccggctttc accggcagca gctgtttttc
     2101 ctgatgtacc tcgccggttt ccaagtccag cagtgccgcg gacgtggtat agttgctggt
     2161 atcaatcccc agaatttgtg ccatcggcag tgtcctcctt taccgccggc ttcgaacggg
     2221 cataggcacc cagtacgccg ttgataaagg cggtatcttc tgcataggca tacgcctgcg
     2281 acaggtgcac ggcttcgctg atgactacgt tcaccggtgt ctgggggaga tttttcagtt
     2341 cgtacagtgc cagccgcaga atcgaacggt tcaccgccgg cacacggttc aggctccgct
     2401 gtttactgta ttcggaaatg atgttgtcca gttcctctgt ctgggacatc acgccggaaa
     2461 cgtactgctt caccttttcc ggaaccggca gttccagcat ttcctctgtg gaagcgtaca
     2521 gatcctcaat ggtggtcagt tcctcggctc ttaccattgc ctcgtacagc agcaaaaatg
     2581 cgtattctct ggtttcgtgt tttgtcataa ctacctccat attcatattc tgacattttc
     2641 tgaaattctt gtccgcctgc aacgctttct gcacttttgc aaaaaagaaa gctgctgtag
     2701 acattttaca gcagctttcc ccatgtattg cttttgtttc ctttggtatc tctatagaat
     2761 tccatagaat cgaaagaaac cgcatttggt atgctgcccg ttccatgcca gtccgaacaa
     2821 gttctttctt ctattatacc aaaaaacaac gccagataca actattttac ctcaaaaatt
     2881 cgaatatttt ctgccggaac cgaaacttcg cccaaaatca cgtctttgat tgctgccgcc
     2941 tgagctgcgt ccagcccctc ggtctggaca acgaccttgg cactgtcccc gtccagatag
     3001 acgatacagt tggaataacc ctgtgcctga atcagggatt cgatcgtgct ttcgctttcg
     3061 atcagcttcg aagtttccac agcggcaatg gcatcggtca ccagttcgtc ctctgtccga
     3121 tctcctgccc ccagcatgga ctgcaacgcc gccacgcttt cgtcacggct ggaagtccgt
     3181 tccagtcttg cctgtgcaaa ataattctcc gcactgccgg cggcggcgta gtcggcttcg
     3241 acctctgcgg cagtttcttc agcaggggct tccccgttgg tggaatatgc cccgtctgct
     3301 gcggattcct ctgtcggtgt gctgtcctct gccgccggtt cgctctgttc cgcttcggcg
     3361 ttgacaaagg cggtgtcccc gtaggtcacg ctgtccttgg actgcacctg tgccgtttcc
     3421 agcttgccgt cggacatggc gtagttgaca tagaccgcaa tggcaagcat cagggtcagg
     3481 cacgagagaa tgatctgttt ttttccgatg atgagagatg gctttttcat aatgtattcc
     3541 tttctggttt tgagcgtgtt gctttgcgga aacgctacgg gaaacgctga ataaagcttg
     3601 tgcgtaagat gcgtagtcag atttttcggc agattgctca g
//
LOCUS       DAWBGR010000213         3606 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_6011_length_3606_cov_3.803999, whole genome shotgun sequence.
ACCESSION   DAWBGR010000213 DAWBGR010000000
VERSION     DAWBGR010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6011_length_3606_cov_3.803999"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          997 a          705 c          860 g         1044 t
ORIGIN      
        1 tgcaagcaga tggcgtgcaa agtcgtcaga cgctctttgt gcggtattgc cctataaaaa
       61 attatctgga taggaggttt ggaacatgct tgacaagctt ttatcttcat tgcaggggat
      121 accggcatat tctattttaa tcagtgctgt tgttgtcatt tttattgctg ttagtgttct
      181 ggtgatgttt ggaactgcac ggcaggaaaa agaaatcgaa cagcttcgtt gggaaaaggt
      241 cagtgctcac atggcattgt acgacacttc acgccgtgtt gtcattccgt tggaagcgga
      301 tgaaatcctg atcggcagac acggagccgc agatattcgt tttacggaca tgagtgtttc
      361 ccgttatcat gcagtgcttt atgtgtctaa cggggtgtgg agcatcatgg atttggattc
      421 caaatccgga acatttgtca acggcaggcg gattcgttcg caggtcaaac tcaaagacat
      481 ggacgagatt cggtttggaa caaaatcagt gataatccgg cagcgaagga ggaaacagaa
      541 tgtataaaaa cggactcact tatggaaaat gcaccacaat tgtcttgttg acacacatta
      601 ttatgctgct tgcgattctg ctccgcggca aatatgatac gccgcaagac attttgccgt
      661 tggcagttgc tgtgatcggg ttagatctca cgtatagtat agtgctgcga ttcctgtacc
      721 gccaaatgac ttatacgctg gattttgtat tgctgctgct tatcaacatc agtgtcatgt
      781 ttcaatcctg ttttggcggt gttggatttg cggcaaagca ctatgttact tgtattgtag
      841 cactggcagt ttgtcaagtt ggtttccttt tgacacgaaa tcatgtttgg attcaatcta
      901 aaaaaattgt gctgtatatt ctgttgggtg tgctgatttt gagtattttg ctgctgactg
      961 gcagtcgaag catgtggatc aacatcggac caatcagtat tcagccgtca gaatttatga
     1021 agccagtttt tgttttgatc tgtgcgacct ctattcggga acagcatgag aagaaaaaga
     1081 ttctgttttt ctacgtttcc aaggaaatga tgatcctgac aggagcattt attgtcattg
     1141 taggtttgca gtggtggtgt cgtgatttag gttcgctgcc tacttttgcc gctgcgtacg
     1201 gttgtgcgtt ggcatgtcgc ttgtgttatc caaaagcaaa attttccaag aaaacaatta
     1261 tcggaatcag tgctattgct gcgattcttg tagcattggc attgcgattt gcaccgggtt
     1321 atgtacaaaa ccgtctttcg gttgacatct ggaatcaaat ggagggagat ggataccagc
     1381 agtgtaaagc tttgattgcg attgcagagg gcggtttgtt cggaaaggga ccgggctgcg
     1441 gctccttgta taaagtgttt gcctcggaca ccgatattgt tttttctacc atttgtgaag
     1501 aatggggctt tttggtggca ttgctgtcga tattgatctt aatggcaatg atgctgcttc
     1561 cgatgatcaa taaacctcgt agctatttcc atacaactat gattcttggc tgcgtggcag
     1621 tgttgattgt gcaaatgggc ttgaacatct tcggctcctg caatctgatc ccgtttactg
     1681 gtgttacaat tccgtttatt tcgcagggtg gtagctccat gattaccagt ggcttcttag
     1741 ccggtatgct gaaagcggga cagtcaccgg tatttaagaa accggaaccg aaaaagaaaa
     1801 aagccaaagc aagaaaggcg gtggctgcgg catgaaaagc attcaaagta tccgacgcgg
     1861 agaacggctg accattctga ttttgattgt gtttttccta ggcatgggtg ttttggtttg
     1921 gaaagtagta aaagaatctt ccttttatat gtcccattct gatgatgtga cgctgggaat
     1981 ggtctatgac cgaaataatc agattttatt tgatccaaat gcttctacag aaacctatga
     2041 cgaaaattac ttcttagatg tgggaaatgt gattggcgat gacagcggtc agatgacaag
     2101 tacgctggtg tccgaaaaca ttgaaaaact gcaaaactat agtttgattt ttggtgcaac
     2161 gcctcacgga aaaacggcaa tttactccac attggatcat aaagccaacc aaacggtata
     2221 caatgctttt ggcagcaaaa acggaacggc aatcgcttac aactatcaga caggagagat
     2281 tctggtgtgc gtcagcaaac ccagtgtcaa tattctggat aattacagca atatcagcga
     2341 attgccggac ggcagtttga tttgcaaagc gttttatgaa accactcccg gctccacaca
     2401 aaaaatagca accactgctg cggctctgga aacattcggt tatgatggat tgatgtccaa
     2461 aacatatact tgcaacggca tctataccac aaaatacaat cagcagatca aatgtcatga
     2521 ccttaacgga catggcacac agaacattgt gcaggggttt gaaaacagct gtaatccgtt
     2581 ttttgcacag ctggtacagg atatgccgtt ggataacatt attcaaacat acacacgtat
     2641 gggcattgcg gtaaacgata caaaaatgca gaaaatccaa tttgacgggt tatcttcttt
     2701 tagtgcttct acaaagctga cagataccag tgacttcgac accatgtgga gttgcatggg
     2761 acagagcgaa gcacttgcaa gcccactgca aatgatgctg tgggagagtg ccattgccaa
     2821 tgccagcgga aaagtgacag agccctatct cattgaccat actactaacg tgactgggag
     2881 cacaaaatat gaagcaaaaa ccacctatgg agagaacaac attttttccg cagaagtagc
     2941 cagtcagata aaacagatca tgcgacaaaa tggacaggaa cgctacagtt ctattggcta
     3001 tcccgttggt gtaaaaagcg gtacagcaca ggtacagaac ggggcatcgg aaaattcttt
     3061 gttggttgga ttcaatgatg atcccaattt accgattgca ttttgcgtag taattgaaga
     3121 ccgtgtttct ggtgaaatct ctacttcgga tattgtttca acaatgctga atgcactgaa
     3181 tcaaaactaa tttgcatcag cacaataaac aaaaaagcgt tttgcaggca gaatataatc
     3241 tgccttgcaa aacgcttttt tgttatgcat aaaatgcctt aaagcaaatt gatttttttg
     3301 aacatgtcct gcatcttctg atcgattcct gaaatagttt cggaacactc tttcagttcg
     3361 ccagtcaggg aagcggcaat tttaggatcg ccagtagact tgtaatgcag ctgatgctcc
     3421 agacttgccc agaagtccat ggcaatggtg cgaatctgaa tctctacagg agcaatttct
     3481 ttgccgttgg acatgtagac cggtacatcc agaatcatgt gaaaactccg gtaaccattc
     3541 ggttttggat ttttgatata gtccttgatt tttcgcagct gaaaagcact gtggtctgtc
     3601 aggagg
//
LOCUS       DAWBGR010000214         3556 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_6119_length_3556_cov_4.970865, whole genome shotgun sequence.
ACCESSION   DAWBGR010000214 DAWBGR010000000
VERSION     DAWBGR010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6119_length_3556_cov_4.970865"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          968 a          891 c          957 g          740 t
ORIGIN      
        1 accaaacggc ccgttggtca agtggttaag actccgccct ctcacggcgg catcaccagt
       61 tcgagtctgg tacgggtcac catttttctt ttagtcggtg tttatggcaa agaggttcca
      121 cccgttccct ttccgaacac ggaagttaag ctcttttacg cctacgatac ttgatcatct
      181 tgatctggaa aaattgggca atgccggcac aagagcacat gtaaatgtgc tcttagatat
      241 tcctctatag ctcagtcggt agagcatgcg gctgttaacc gcagggtcgt tggttcgagt
      301 ccaactggag gagccaagat aagcgaatca cgaaagtgat tcgctttttt gttttatata
      361 tgatgaattg aaaaaaagcc cgattcagtt ttcaccgcaa aggcaaactg gatcgggctt
      421 ctttctttat acagataaaa caaaaaccgc cgcaatatca tgcgacggtt tttccatttg
      481 gcaggggcac caagaatcga actcgggaca cgtggttttg gagaccactg ctctaccagc
      541 tgagctatac ccctatgaaa attattttgt acaggaaacc tgcaacgtaa attattatag
      601 cacaaaagaa ccgccttgtc aactactttt tgcaattttt ttcatttgac atttgtacac
      661 ttcgtacata tgtccgaaaa tgtacggaat tccggcagaa tcggggcaga aaaggggaaa
      721 actgcggcac gattccgaca aaagccgaca gcagaatttg atataataga cctgtcggaa
      781 cgcagaaacg gcaaaagccg gaagccatcg gcgatcgcaa tggctttgcc gcccgacagg
      841 tctgacaaaa ggaggctttt tcatatgcag aatctttgga aacaggtggc agaccaatat
      901 tgcacctctg cggaggaagt gcaggcggaa atcgcagagg caatccggca gggcatgaac
      961 aatccggacg cggacgtgca gcaggcgtgg gcagaaatcc cccgtcagca ggacgagccg
     1021 cagccggagg acgttgtcgc atggattgcc gcacagctgc tggcggcggg aatcctgccg
     1081 gaatccgcag ctgtatcgga acagccggaa atggcagtca aacggtaatt cccaaatctg
     1141 tacatctttt gcggaaaatg tgccatattc ctgaaaccac gcaggggacg gtgaaaaccg
     1201 tccttttgca ttttctggag aaacagtttc gctgggaacg tgttcttctg cattcataaa
     1261 aattccatgc aaactgcccg aaaagccctt gcatctccgg aaaaactgtg ctatactaat
     1321 attatgcagc ctcagcgaaa atatgcttcc ggctgttgag atcgagagaa agaagtgcag
     1381 ataaaaatga caaaaattac aatattctcc ggctttttgg gtgccggtaa aaccacgctg
     1441 atccagaagc tgattcagga aggctatgtt ggcgaaaagc tggtgctcat cgaaaacgaa
     1501 ttcggcgaga tcggtattga cggcggcttt ttgcaggacg ccggcgtaga gatcaccgag
     1561 atgaactccg gctgtatctg ctgtagtctg gtaggcgact ttggcaaggc tctggaaaag
     1621 gtgctggaac agtaccaccc ggatcgcatc ctgatcgaac cttccggcgt gggcaagctg
     1681 agcgatgtca ttcgtgcagt ccagaatatc catgcccatg acgtggaact ggacggcttt
     1741 accacagtag tcgatgccaa gaagtgcaag atgtatcaga agaacttcgg cgagttcttc
     1801 aacaatcaga tcacctatgc aagctgcctg atcttgagcc ataccgctgg tctgtcgcag
     1861 gagaagctgg acgactgcgt tcgccgcctg cggacctgca acgaaaaggc tcctgtcgtc
     1921 accacggact gggcagaact gaccggcaaa cagctggttg aggcaatgac ccagaagaac
     1981 acactggacg acgaattgca ggaactgctg gcagaagccg ctgcacacga tcaccatcac
     2041 cacgatcatg accatgagga acacgaacat catcaccacc acgatcatga ggaacatgac
     2101 gagaactgca cctgcggctg tcacgatcat gaccacgacc atgaggaaca cgaacatcat
     2161 caccaccatg accacgagga gcacgacgag aactgcacct gcggctgcca tgaccacgat
     2221 cacgagcatc accaccacca tgcagacgaa gtgttcacca gctggggcgt agagtctgcc
     2281 cggaagtaca ccaaggagga gatcaccaac gctctgacgg ctctggaaga cgcagacaag
     2341 tacggcatga tcctccgtgc caagggcatt gtaccggcag aggacggcag ctggatccac
     2401 ttcgactatg taccgggcac accggacgtt cgcagcggca gtgccggcac caccggacgg
     2461 ctttgtgtca ttggctccgg cattcacgaa gatgccctga ccgaactgtt ccacgcagaa
     2521 taaaaggagg acaacatcat ggcaggagga caacagcctc aggaaaatga cgttccggta
     2581 tacctgttta ccggctttct ggaagcaggc aagaccaagt ttattcagga aaccttagag
     2641 gacaagcggt tcaattccgg cgaaaagacc atgctgctgc tctgtgagga gggcgaggag
     2701 gaatacgaca tttccgctat gccctgtcag gagatctata tccgcaccat tgaggagccg
     2761 gaggaactga atccggcaca cttacaggaa ctgctgaaag aatgcggtgc cacccgcgtg
     2821 gtactggagt acaacggcat gtggcagctg caacagctgt tccagaacct gccggacgac
     2881 tgggcagtgt atcaggaaat gttctttgcc gatgccacca cgttcctgca atataacgcc
     2941 aatatgcgga gccttgtggt ggacaagctg aacacctgcg aactggtagt attcaaccgg
     3001 ttcggcaagg acatggacaa aatggaattt cacaagatcg tccgcggtgt cagccgccgg
     3061 acagatattg cctatgagta tgcagacgga cacgtggaat acgacgagat cgaggatccg
     3121 ctgccctttg acgtaaatgc accggtgatt cggctggcag acacggacta tgctctgtgg
     3181 tatcgggaca tcatggaaga tccgaaaaag tatgacggca agaccgtttc attccgcggc
     3241 attgttgccg tagatccgaa attcccgccc aatacgtttg ccgtaggcag acacgtgatg
     3301 acctgctgtg tggaagatat tacgtatagc tgcgttgtgg cagagtggga aaaggcaagc
     3361 gtgctccaga cacggcagtg ggtgcaggtg accggaaaga tccacgtgca gaagcataag
     3421 ctgtaccgcg gcaagggacc ggtgttgcag gtgcaggaag tcgtgatgac aagtgcaccg
     3481 gaacaggaag tggcaacgtt ctattaaggc aataccgcac aaagagcgtc tggcgacttt
     3541 gcacgccatc tgcttg
//
LOCUS       DAWBGR010000215         3535 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_6162_length_3535_cov_5.585920, whole genome shotgun sequence.
ACCESSION   DAWBGR010000215 DAWBGR010000000
VERSION     DAWBGR010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6162_length_3535_cov_5.585920"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          707 a         1057 c          977 g          794 t
ORIGIN      
        1 caaccggcgt tggcaagccc caggcggaca gcgagggcaa cgagcgattc cgccggacgg
       61 tttcgacatg gctgcactag aacacacaaa cagtttacgc aacaggctcc cctgaaaggg
      121 gagctggcag ccgtaggctg actgaggggt ttcttccgtg aaatctgaaa caggagtaaa
      181 agatttcagc agaacccctc caccagcttc gctggtcccc ctcgtactga gcggtcccct
      241 ctgccctgcg ggcatctccc caccccgtgg ggagtcaccc ctttcagggg aggcttatgc
      301 acaagcctgc ggggcgatgt gggcattgcc cctacttatc tcaccccagc ggctgtttct
      361 tgatcttatc gctgcggcga gggtacttct gcggggtagg ggcagttttc cggatccgga
      421 tcagcttccg ttcaccggca gttggcagag tatagctttc ttcgccgacg atctcgccgc
      481 ccagcagctt cgccgctgtt tctgcctgct ccgccgtttc cgacggtcct ttcattgcgg
      541 caaatacgcc gccctgcttc acaaacggca ggcagtattc cgtcagcacc ggcatggctg
      601 ccacggctct ggcagtcgcc atgtcaaact gctcccgcag ttccggcagc ttcgccccgt
      661 cctcggctct ggcgtggata cagcggacgt tggcaagtcc cagctttgcc gccgtctgct
      721 ccagaaaccc gatgcgtttt tgcaggctgt ccaacagtgt cacctgtaaa tccggccgca
      781 gcagtgccag caccatgccg ggaaagccgg ctccggtgcc aacgtcaata caggtcgccc
      841 ccagcggcag ctgcaccttt tgcagcaaga tcgcacagtc cagaaaatgc ttctcccaag
      901 cctctgccgg ctcggtgatc gccgtcaggt tcatgtgccg gttggtttcc gtcagttccg
      961 ccagatagca ctggaactgg gcaaagtggc tctccgtcag gggcagtcct gctccgtcaa
     1021 acagctgctt cgccgcttcg tatgtcatca tgcatttccc tcctttgcag cgtcccagac
     1081 gatcagcacg gaaatatccg ccggtgatac gccggagata cggctcgcct gtcccagatt
     1141 ctggggacgg tgggcgttca gtttttccgc agcttccagc cgcagcccgt gaatggtggt
     1201 gtagtccaag tcctccggca gcttccggtt ttccagcttc cgcatgcgtt cgatctgttc
     1261 cagctgcttc ggaatatagc cgtcatactt gatctggatc tccacctgtt ccccaatcac
     1321 cggttccagt gccggacggg tggggtcaaa aggagcaatg acagcatagc ccagctgggg
     1381 acggcgaatc aggtctgcga ctttacaacc ggtagtgatg ccggaggtgc cgtgctgttc
     1441 cagaaacgcg ttcagttccg gagcaggtgc cagattggtt ttcagcaccc gctttttctc
     1501 ctgtgccaca agacggtatt tttccagcat gtgctgatag cgttcctcgg agagcagccc
     1561 cagttcgtgt cccagcggca tcagacgctg gtcagcgttg tcctgccgca ggatcagccg
     1621 gtactcgctg cgggaagtca tcatgcggta gggatcctca cagcccttgg tcaccagatc
     1681 gtccaccagc gtgccgatat aggaagtggc tctgtccatg acaaacgccg gcttgccctg
     1741 cactgccagt gcggcattga tgccggcaac cagtccctgt gcggcagctt cctcgtagcc
     1801 ggaactgccg ttgaactgtc ctgctccgaa cagtccggcg atcttcttgc tctccagcga
     1861 tgcccgcagt tctgtggggt ctacgcagtc atattcgata gcatacgccg gacgcatgat
     1921 ttccacgtgt tccagtccgc ggatcgtccg cagaaatgcc agctggacat cttccggcag
     1981 agaggacgac atgccctgca aataatactc ctcggtttcc agccccatcg gctccacgaa
     2041 aagctgatgc cgcggcttgt cggcaaagcg gacaattttg gtttcgatag acgggcagta
     2101 gcgggggccg atgccctcga tctgtccgga gaacagcggc gaacggtgaa gattttcccg
     2161 aatgatgcgg tgggtttcct cgttggtata ggtgatgtag caggaaacct gattgtgcat
     2221 ccgggacgga tcagagccga atgaaaacgg cacaatgtcc tcatcgccgt cctgccgttc
     2281 catggcatca aagtcaatgc tccggcggtg gacacgacag ggagtaccgg tcttgaaccg
     2341 ccgcattcgc agccccagtt cctgtagatt ttttcccagc ggacggctgg gaagagccgc
     2401 atcgggaccg ctttcgtagg aaacctgtcc cacgtggatt ctgccgttca gatatgtgcc
     2461 ggtggcaact acggcggcgg aaacggtgta ctgtgctccc agcgaagtga caacaccggt
     2521 cactctgccg ttttcggtct ggatctctgc gatctctgcc tgcttgatct ccagcagggg
     2581 tgttttttcg cagacttgtt tcatgtagtt gtggtagcga acgcggtctg cctgtacccg
     2641 caggctgtgg acagccggcc ctttgccgcg gttcagcata cggctctgga gaaagcaggc
     2701 atcagcagcc tttcccattt cgccgcccag agcgtcgatc tcccgcacca gatgcccttt
     2761 tgccgtgccg ccgatagacg ggttgcaggg catgttggcg atctgatcca gagatatggt
     2821 aaacagcact gtgcggcagc caaggcgggc agaagccaag gcggcttcta caccggcatg
     2881 accggcaccg atcacagcga cgtcataagt tcccatgtga taatattcca taggtatcac
     2941 ctcaattgat atgaatattt ataaatattg cagacaaatc ctgttcgccc gcgaaaagat
     3001 ttttgtgcgg cgggtcagcc caaggatagc aagccgcctg ttcgccccgc cgggggattt
     3061 ttgcggcgtg taatcttcgt gtgatacttg accttgtagt acaagtgaaa cgcgaactct
     3121 tttctttttt tgcttctttt tttcttttca gcaaagaaaa aaagaagcgt aactccttgt
     3181 gaaaccggct ggctgctgta gcgaataccc ctcagtcaag cctacggctt gccagctcag
     3241 cacaaggcac gcccttcggg tgccctaaag ggaagcctta ttcacaagct tgcaaacgcc
     3301 gtatgaaata aagttacccc acttacataa gaaaaatact tgaatatcaa cactacatgt
     3361 acaggcaagg gcaactagcg gcgttggcaa gccccaggcg gacagcgagt gcaacgagcg
     3421 aattccgccg gacggtttcg gtgcggctgc actagaaagt acactttgtg ggagaacccc
     3481 tccaccgcct agcggcggtc cccctcccct ttcaggggag gcttatgcac aagtc
//
LOCUS       DAWBGR010000216         3530 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_6170_length_3530_cov_4.183022, whole genome shotgun sequence.
ACCESSION   DAWBGR010000216 DAWBGR010000000
VERSION     DAWBGR010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6170_length_3530_cov_4.183022"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          820 a          899 c         1041 g          770 t
ORIGIN      
        1 cccattgtgc cacccgtctg cgattggttg tcaaagacga aaagagcatt tgcaaagagg
       61 agatcgaaga aattgaaggc gtcaagggcg tatttatcgc acagggacag ttgcagatca
      121 ttttcggcac cggcgttgtc aatcgggtct atgaggaatt cgtagttgtt tccggcattg
      181 aaaccgccac aaaggaagat gtcaaggcgg cagccggaca gaagatgaat ccgttcaaga
      241 aagcgatcaa ggtgctgggg gatattttcg taccgatcat tcctgcaatc gtggcaaccg
      301 gtttgctgat gggcttgctg gagggcttca gtaccttgtt tccggcaatg gcaaattcgg
      361 atacctataa gattttccac atgttttcca acactgcatt tacatttctg ccgatcctga
      421 tcgccaccag tgcggcaaag gcatttggcg gcaatatgtt cctgggttct gtaatcggca
      481 tgatcatgat ccaccccgac ctgatgaatg cgtggagcgt ggcatctgcg gtggaagccg
      541 gcgaggcaat cccgaagttc tccgtctggt tcggactgta tgatgtcaac gcggtgggct
      601 atcagggaca tgtcattccg gtaatcattt ccgtatggct catgtgcttc ttggaaaagc
      661 ggctgcataa gatcgtgccg gcatcaataa atttgtttgt cacaccgctg gtcagcgtgg
      721 gcgtgtccgg ttatctggca ctgacggcta tcggaccggt attctcccag ctggaaacat
      781 gggtactgga cggcgtgcag tggctgattg cagtgcccta cggcatcgga gcattcctca
      841 tgggcggact gtattcctcc acagttgtgg ctggcgtaca ccacatgtat acgatcattg
      901 acttgggaca gctttcccag tacggcgtga cacactggct gccgctggca tcggctgcca
      961 atatggcaca gggtgcagct tgtctggcag tgggactgaa aagccgtggc aatcagaaga
     1021 tgcggtcgct ggcatttccg gcagctctgt cctgcatgat gggcatcacc gagccggcaa
     1081 ttttcggtgt caacctgcgg catatcaaag tgtttatctg cggttccatc ggcggagcat
     1141 gcggtgcact gatgacctcg attttccatt tgggagcaac ggcgaccggc gtaaccggta
     1201 tcttcgccat tctgctgcac ctgaaccagc cggttcagta tatcgtttcc atgctggtgg
     1261 caatgggcgt ggcattcctg ctcacatgga tctttggcta taaggcagaa aaaccggcag
     1321 cggcaaagac tgcatcgaat gcgtgaacgg aggcagaatc ctatgagtac tgttagaaca
     1381 cagaacctga aaaatgcttg tcgggcattg gaacggagca ccgaccggac ttcgacgtgg
     1441 cgaccggtgt ttcatctggc tcccgtgacc ggttggctga acgaccccaa cgggctgtgc
     1501 cagatcggag aaacccatca catctttttc cagtattctc cctttgatgt caagccgggg
     1561 ctgaactact ggggacacta taccacagaa gattttatcc actaccagta ctgcgaaccg
     1621 gcactgtgca gcgatgaaaa gtttgactgt cacggcgtat actccggttc tgcactgggg
     1681 caggacggca ccatgtacct gttctatacc ggaaacgtgc tccggcggga cagagaggac
     1741 tacgactata ccactgccgg cagagaacac aacaccattc gggcagagag cgaggacggg
     1801 ctgcacttca ccaaaaagac ggtgctgctg tacaacgagg actatccggc agacctgacc
     1861 tgtcacgtcc gtgacccgaa ggtctggaaa gacggcgaca gcttcgcaat ggtgctgggt
     1921 gccagaagaa aagacgacgt aggcgaagtg ctggtgtatc gttccgacga cctgaaagcg
     1981 tggacgctgg caaaccgcat cacctctcag aagcctttcg ggtacatgtg ggagtgtccg
     2041 gactgcttcg aattgcaggg aacccgcttc ctgtcctttt ctccgcaggg cgtggcggcg
     2101 gaggactgga agtatcagaa catctaccaa agcggctact gccggctgga gggtgacctg
     2161 tacggggaat acaccctgtc tgcatttgag gagtacgacc acgggtttga cttctacgct
     2221 ccgcagacct acacagaccg gcagggacgg cggatcctga tcggctggct gggtatgccg
     2281 gacagcgact acggctatcc ggaacgggaa ttcggctggg ccaatctgct gactattccg
     2341 cgggtgctcc atgagaaaaa cggcaggatc caacaggagc cgctgccgga attgcagcag
     2401 cttcgcggtg cggaacagac tgtcgttgta cagcaggaat tctcgtgcag gaccgcagcc
     2461 gcttatgagg cagagattcg cctgaccgcg ggaaagtccc tgcgggtgca gttcggcaca
     2521 gacggcgtgc tcacatggga cggcagcttc ctcacactgg aattcggagc aagcggctgc
     2581 aatcgcccca tgcggcaggt accgctggaa cggctggaac agctgcggct gttctgtgac
     2641 acttcttctg tagagatttt tgccaacgag ggcgaagccg tgttcacgtc ccgcttctat
     2701 gccgatgcag agcagtcgaa gctgacagta caggcggagc aggcagaggt acacctttgg
     2761 gaaatgcagc cgttttacgt agaaaatttg ttttccggcg gcaagcaggc gtgacaggcg
     2821 ggaatgcgta tgaaacagat attggcaatt ggagaagcac tccgccagcg gctgtttgcc
     2881 ggctcctgcc gtatacggct ttactccatg ggaaaaggcg gtgccattgc atcatactcc
     2941 gaccgagaaa catttctcgc gtggaaacag aacagaaagg attgagcagt tatgggaatg
     3001 cttgattttt tgaaaaagaa taaagcggca gcagacctgc cggaggctcc ggcgaatact
     3061 ttgctggcag tgcagaacgg caagatcgtt ccggtagaca cactgccgga tccggttttc
     3121 gcagaaaagg tactgggtga cggctatgcc attgacccga ccgacggcaa ggtgctgtca
     3181 ccggtttccg gaaagatcgt agacatacag gattccctgc acgcttacgg cattgaaaca
     3241 cctgacgggc tggaagtgct ggtgcatatc ggcatcaata cggtttccct gaatggtgcc
     3301 ggcttccgga cgaagcacaa ggtaggcgat accgttcacg ccggcgaatt gctggcagag
     3361 gtgaacttga atttgttgga gcagaaccag ctgccaaagt ttacagttgt cctgatcacc
     3421 aacatggacg cggtgcatgt gatccgtccg aattatacag ctgccgaagc cggaaagact
     3481 gcggcactga cttacgagaa atagggcaat accgcacaaa gattgtgcgg
//
LOCUS       DAWBGR010000217         3515 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_6195_length_3515_cov_3.888150, whole genome shotgun sequence.
ACCESSION   DAWBGR010000217 DAWBGR010000000
VERSION     DAWBGR010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6195_length_3515_cov_3.888150"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    227..236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          960 a          858 c          760 g          927 t
ORIGIN      
        1 cctcatttta acacatacag tgaaaaaata cacggttttg cactgaatcg cctgagattt
       61 gaaaaattga tagttccgtc ttttttgcat ttttttgtaa agcaaacgat gcaggatgtc
      121 gaatcgtgtc gtattctgtc gaatccgaaa ctttttttcc aaaaaaagtc tggatttcca
      181 ttgatttttt acgcaccata tgttataatt ataataaatt ctatatnnnn nnnnnnaaaa
      241 tagaatagtc gcatttccga tttgtaaaaa acatgtaatt acacaagaaa acgacgtata
      301 ggcaaactga ccgtgcgtaa agccgacagg cagacattcg tgcggcattg cctgcaacac
      361 acattgtcaa attcagagat tccataagca acactgcaca gagccgccat gcgggatcct
      421 gtacagtatt gcacacagaa caggaggtct atccaatcac atggttccaa tgaaaattgc
      481 tattgcagac gatgaactgt atgtctgtca gcagattcga gaatgccttt tgcaatacag
      541 tacacaaaaa aacgtggaca tggatgcacc ggacatttac accgactgcc gcacgctgta
      601 caacaccgat ctgagtggca aacgctatga tgttgttttt atggacattg acttttccgg
      661 tgaaaacaac atcgccagac ttttctctga cagctatgct gtacgggaac ggtgctctga
      721 cggactggca ctgggcacgt acttgcgaca catactggga catcagaatt ttgacattat
      781 atatgtgaca tctcatgaaa attatgccat ccatgccatt cctattcgtc cggcagcatt
      841 tgtgcaaaag ccggtcactt ttcagaaaat tgcccgtgct ctggacgata tgctgtatta
      901 tcgggacatt tcccgccgca tcttcaaatg taccagcaac aaatctctgg tgcatgtgca
      961 ggtgtcccat attcgctatc tgaacagctg cggaagaaag gtgtatctgc aaactacgga
     1021 ttcccgcatc tccttttacg gaaagctgtc agaagtacag caggagccct gcttcagttc
     1081 atttatccag atccacaagt cctatctggt caatccggac tatatcgacc atatttcctc
     1141 caacaccgtc tttttgcagg gcagttctca tgaacagctc cctattagca gagcctatca
     1201 gccgctggta gagcagtggc tcatgaaggg ctaagtgcca tgaaacagta tatcacgatc
     1261 atttctaata ttttccacat ttacgcagtc tatcagtttt ctcaactgct gtttcaaaag
     1321 gaccccagcc ggaaacggac agagatcctg gcgtatatcg gatatttttt gctgaacagc
     1381 ggctgtttta tcttgtttca caatattgtg ataaatgttc tcagcaatat tctcctgttt
     1441 ttctgcatta ccctgctcta tccgaccaca tgggcacacc ggatccttgc aactgtttcc
     1501 atttatatca tcagcatggc agcagatatt ctctgtacca cgatctcgat ctggtgcgag
     1561 tataccctgt tcttcaccag cggattcgct gcctcacttc tcatgctgat cattgcacgc
     1621 atgagcctgc actttcatct gtacaaaagc gatgtcattc cgcggctaaa catattttat
     1681 gtttttaccg ccattttcat tcccttgggc agtatccttc tggcacacta cattgcccgc
     1741 acactttcat ggcgttcctt ggtggctgcc attgtccttc tgctgatcaa tgtcagtgtc
     1801 ttttccctct atgaccgcat gattcacctg atgcaggagc gtcacaccgc actgctgatt
     1861 gaaaagcaaa acaaagccta tcagcaacag ctgcaattca tgcagaagag ccagatgcgg
     1921 ctgcggtttt tacggcatga catgaaaaac cacttattcc agatcaagca ttttctggaa
     1981 accaacgaca tgaagggaat gtacacgtat atcaacgatc tggaggataa tctggatctg
     2041 acccagagca tcacctacac ccagcatgaa gccatcaaca gtattctgaa ctacaagctt
     2101 ctgccgctgc ggacagacgg cgttgcactg gaagtagacg tacagctgcc ggatcagctg
     2161 acatatccca tttttgattt gaatgtactg ctgggcaact tgctggacaa tgccattgaa
     2221 gccactgcat tggtgactcc gaaagaaaat caaaaaatca cactgaaaat gaaagaacgg
     2281 ttcggtgccc tgcaactaca ggtcggaaac cgtttcaaca gcaatattgt acctaaaaac
     2341 ggcacacaca agtcggacaa gatcaaccac ggactaggac tgaaaagcgt acaggaaatc
     2401 gttcggcgac atcacgggaa aatgtggatc gttccggaaa aagactggtt cgagatcaat
     2461 atcatcctat atgattctaa tccggaaaaa ctgcaaggtt ccggaaagtc tatataaaaa
     2521 gcaacaccgc ataactattt atgcggtgtt gtttttcgtt tttcactgca cgattcgcag
     2581 tttttcctca aaggaatcgt gcagcagcag cttctggtat tttttcggtg tcagaccggt
     2641 ataggctttg aagtcatgca caaagtgcga ctgatcaaaa tatcccagat ctgcggcaat
     2701 ttccgttaaa gaacgcccct tgtgctgatt ctggttcagg gcagaaaccg ccgcctgaaa
     2761 ccgtacaatt ttggcaagct gcttcggact cagacccgtt tcattgcgga agcagcggtc
     2821 aatataccgt gcagtataac cggtgtcctc tgccagctgt tcaatggaaa tgctgccggc
     2881 agagcggatc agcaggttga atgaatggag caccagcagg ttgctgtgtt ccatgggaca
     2941 ggcacgacga taaatgttca tgtaggaacg catgaatgcc cgaatctgtt tgcggaaatc
     3001 ggttgtgccg cagattccct ccagcatgcg gcgatcctga atcaactcgt ccagcgggat
     3061 ctgatgattc accagctggg acatgatctc cgaacttccc agaaccggat tgtaccccgg
     3121 caaaaaccgc acgccgaaat agcaggtatc ggagtgcagc agaactgcct gcggagccag
     3181 aacagtgccg cagatctctg cgtaaggctg cttgggatca cagcaaaata ccatgtccac
     3241 acagccgtcc ggtacagcat agcggggttc cgttcccgta aagcactgat aaaagtgcac
     3301 aatgccgtac cgatttaaaa taaactttgc gtatgctctc gatgatgtca caaaaaatgg
     3361 ttgcttgggt gtcatataaa tctgttgttc cattgcggtc cacttcccat ttttcgggca
     3421 gacttgacag gcgtgccttg tgcaggactg cccttgcttc ttcaaggcaa taccgcacaa
     3481 agattgtgcg acagatgcgt cgtcaaattt ttcgt
//
LOCUS       DAWBGR010000218         3489 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_6249_length_3489_cov_11.306639, whole genome shotgun sequence.
ACCESSION   DAWBGR010000218 DAWBGR010000000
VERSION     DAWBGR010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6249_length_3489_cov_11.306639"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          782 a          987 c          841 g          879 t
ORIGIN      
        1 acgggaaatt tgcaagcaga tggcgtgcaa agtcgccaga cgctctttgt gcggcattgc
       61 cttatctcaa tgcagtccgt aagccttatg cttcttcctt catctttgca tagcatgcac
      121 tgcagtagat cggacggcct tcacggggct ggaacggcac tcttgcttcg ccgccgcagg
      181 aatcgcagac agcagtgaac atttcgcgtt cctgtctggg agcaccgcca cgctttgcat
      241 cacggcaagc cttgcaacgc ttcggctcat tggtgaagcc tctttctgcg tagaattcct
      301 gttcgccagc agtgaaaacg aattcattgc cgcagtcctt gcataccaaa gtcttatctt
      361 cgtacatagt ataaccttac ctttctcgag ggtgtccgct tgggattcct cgtgccggaa
      421 tggtctttcc ggctgttcca tgaaaaatgt tccggcggca ggataccgtc cggagaatcc
      481 cggtgcaatt actgcaaaac gccttccgaa tgaagttttc gtcgggcacg ctgcagtgca
      541 ttatccactg attttacaga gattccgagc tgttccgcag cctgtgcata ggacgctcct
      601 ccgtagatca gcatgcaaac ctgatattcc cgccgggata agcggttcac cattgcacag
      661 aacatctccg ccgcctgtga ccggctctgt accacagaat ccggcggcag ggcggtgtcc
      721 tgtacctgag aaagcggagg catgtccgat gcccccacag gtaacggtaa attgcggcag
      781 tgccgcaacg cagatacaat gctgtttgta atgcatttgc ccgcataggc agaaaaaggt
      841 acgccacgca tgaaatcata ccgacatacc gcagaaatca gacccaaaaa gccctcttgt
      901 gcgagatcct ccggttcaga aatgccctga aactgccgca cctgatgcca gacggcaccg
      961 gtatagcgtt tcaccagcga ttccattgcc ccacggtcat ttctcgccag gagcacaagc
     1021 tcctgatccg gtagttgctc aaaaggtacc ggcttcaata cgcattcccc tatcaatccg
     1081 tgaaattatt cagtttgcta ttgctcagga agtgcggcac gcacccgttg acggcacgca
     1141 ctgcactttt cttattatat cggaaaacgg cagaattgtc aatatctatc ctcagaaatc
     1201 ttgaaaccct tgggaaaacc ggcatttccc ccagttttgc cggcggcttt tctgacaata
     1261 tgtacagttt tatgaaaggc aacacaaaac ggcagccgtg tcgggctgcc gctggttctt
     1321 tttcagggaa tattttctca cttccgcttc gaacgatttt ttcgttcggt tccacgcttg
     1381 atgtcgcaca tgcgttcctc gctggtctgc ttgaagcggg aaagcatatc ctcgaaagac
     1441 atttcagagg gatcctgccg ctttttcggc tcccagacgt tgggcttgct gcggcggggc
     1501 ggacgggact gctgtccgtc accgtttccg cggaacggct tgccgccgcg ttctccgcgg
     1561 ggagcgtggt cagagtcact gcgtctgccg ccgtgctctt tgttttcttc cgcctgcttc
     1621 atggaaagac tgatcttccc ctgtggattg gtgccgatca ccttgacacg taccgattgc
     1681 ccctctgtca cgaaatcgcg gatctcgttg acatagctgc gggagatctc agagatgtgc
     1741 accatgccca tcaccttttc ctcctccggc atttgcagct ccacaaatgc accatacttg
     1801 gtgatatttt ttactgttcc ctcataaatt tttccgattt cgacctgcat gtaactccgt
     1861 atccctttct ctcgcatttc attgctgggt gttttcctag ggcaataccg cacaaagatt
     1921 gtgcgacaga tgcgtcgtca aatttttcgt cagatgaaac aaaaagcgga gtgaatactt
     1981 gctgtattca cgagcatttt tgtgaaatat gacgggaaat ttgcaagcag atggcgtgca
     2041 aagtcgccag acgctctttg tgcggttttg ccctagtttc tggatgtgtc atagaaccgg
     2101 tactcgtccg gataagcata gttgtattct tcccgtgcca gacgctccat atagccgtca
     2161 atatcgccgc tttcgaggat ccgctgcaaa tcctcgttgt ccgcggtgag atcgtcgatc
     2221 tgtttctgca cctgttccag ttccgttttc ttttccgcga tgctggactg attcgacaca
     2281 atgctgatgg tacagccggc gatcgcaaga actaccgctg tccggctgat gatccgaaag
     2341 atcaagcgtg accgcttttt ttccgttttt gtttcctctg ccatgataac gccgcctttc
     2401 gtttcaaaat tccactatca ttatacaaca tttttctatc tctctgcaag ggtaaatgcg
     2461 aaaaaaaaga tgcttttcga aaaagtttgg cgaaatgtcc aaatttccgg tgcaacggag
     2521 tgcaaatacg cacgacaccg tggaaaagcg ggcgaaacag ccgccgcaaa ctgccgaaaa
     2581 tccccgccag aacccgtccc agaaagggca ctgtgacgca gcccagcgta caacggtaca
     2641 gcaaaaatcc cagcacgctg ctcatgacat aatagccgcg gaattctccc cgtgccagca
     2701 cggtggaaaa acagagcagt gctcccgccc acagcagcag gaacgccaca tcttccagag
     2761 ccgccgccag cttgccgtgg ggcagcagca gccggagcat ccgcagcaca tcgaacacca
     2821 cgcccagcgg gattcccaga agcaccgcaa agatcagcaa ccgtgtctgt tccggcacgg
     2881 tgaaaaaagt ttccagctgc atcatcggaa cagctttccc agcagggaca gcggaccgcg
     2941 gcgatccttt tccccgtatt gcagcagcca gatgtcgccg gtaatggtca tgtcgccggt
     3001 ttccacgctg acatcattga tgtggagatt ccgcccttta atggtcagct cccccagccc
     3061 cgtgaataga ttaatcatgc gttcatcaaa gcagtccaca tcagtcacac ccgtcagcgt
     3121 cagcttggca cgatcttcca gaatgaggtt gtggtgtccg gtgggtgcag gcagttgttc
     3181 ttttttggtc atatcaattt cctcctgtca catggtcttg cattcgtttc agtgtatgcg
     3241 gcgaggagtg aaaacatgct tgcggcggca ttcaggcacc cttttcaaaa caggctcccc
     3301 tctaggagag ctgtcaccac aggtgactga ggggtacttc tgtaaactct tttacatcaa
     3361 gcaaaagatt tcacaagaac ccctccaccg tctaacgaca gttcccctcc cctttcaggg
     3421 gaggttgatt tttattcagg ctctcacttt tgtgcatagg ctccccttta ggggagctgg
     3481 caagccgca
//
LOCUS       DAWBGR010000219         3471 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_6287_length_3471_cov_3.971897, whole genome shotgun sequence.
ACCESSION   DAWBGR010000219 DAWBGR010000000
VERSION     DAWBGR010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6287_length_3471_cov_3.971897"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          797 a          995 c          957 g          722 t
ORIGIN      
        1 ctgcacgcag attgcgtgca aagccgtcag gcgggctttg tgcggtgttg ccctaaggaa
       61 acagtgcaca cgttcacctt aaagaaagga atcacaacac tatgtcaaat aaagtcaaag
      121 tcgtgatctg cggcaaggat ttcactttgc agaccgcaga atcctccaac tatgtcttcg
      181 gtctggcacg cacgctggaa tcccgcatca cggaaattac cgatgccaac agcagtgcct
      241 ctccgtttac cgcggcaatt atggtcgggc tggcaacgct ggacgacctg aacaaggcaa
      301 atgccaagct ggacagcatt cgggatcagt ccaaggaata tgtggacgaa gccggaaaga
      361 cccgtctgga acgggacgct gccatgcagc aggtggaagc cctgaaatcc cgtatcgcag
      421 aactggaacg ggaactccgt gagaaagact ccaaatgaga acacctgaaa ttttagcacc
      481 tgccggctcg gaagatgcct tgctggcggc tctgcggtgc ggtgccgatg cggtctatgt
      541 gggcggaaaa caattctccg cacgacagaa cgccgccaac ttcgatctgc cccaattaca
      601 gcgggcagcg gaactctgcc acctctatgg ggcaaagctg tacctaacag tcaacacgct
      661 ggtatttgca ccggagttcc ctgcactggt acagtttctg gaagcggcga tccggctggg
      721 cgtagatgca tttctggtac aggatttcgg actgctggaa ctgatccggc agatctctcc
      781 tgacgtaccc attcacgctt ccacgcagat gaccattcac acgccggagg gtgccttctg
      841 ggcaaaggag cacggcattt ccagagtcgt ggtttcccgc gagctctccc gcaaagaaat
      901 tgccgcgatc tgtaaaaccg gcatcgaagt ggaaaccttt gtccacggtg ccctctgcat
      961 gagcgtatcg ggacagtgcg gactgtccgc catgatcggt tcccgcagtg ccaaccgcgg
     1021 cagatgtgca caggcatgcc ggctgccctt ttctgcaaag ggtgataaaa acggctgtgc
     1081 cctctctctg aaagacctct gtctggtgcc ttacgtggca gaactggcag aggacggcgt
     1141 ggcatcgctg aaaatcgagg gcagatgcaa acggccggaa tacgttgccg ccgcagtcac
     1201 tgcattagtg caggcacgtg ccggagaaac gccggatctg gacaccctgc gggcagtatt
     1261 ctcccgcagc ggattcacag acggctatta taccggcaaa cggcagcaga tgttcggcac
     1321 ccgccaaaag gacgatgtgc tccaggggca gaaagtcctg ccccagctgg cacagctata
     1381 ccagaagccc acaccgcaga ttccgctgga catgcatgtc actgtacaga ccggtcagcc
     1441 ggcagtgctg aaactgcggg acggcgacgg tatttccgtt tctgtttccg gtgatgtggt
     1501 acagccggca cgcacgaaag caaccgatct gccccagctg gaacgacagc tgggaaagct
     1561 gggcggcacg ctctataccc tgtccggttt gcaggcagac tgcgacggaa ctggcatgct
     1621 ccccgcttcg gcgtggaacg ctctgcggcg ggagggcatt gttcaaatga acgccgccag
     1681 aatcgctgcg aatacgccaa agtataccat gcggcacatc acgctgcccg cacttccagc
     1741 agccggacaa cacaccatac aataccgcat catccagaag cagtgggaca gccagtcagc
     1801 cacactgctg aagcagcctg acgtggaggc actgctgctg ccggcggata cggtttccga
     1861 tgctgtgccg gcagtctgtc gggaacgaat tttcctgacg ctgccgcggt tttgtgcaga
     1921 cgaagccaaa gtgcgtctat ggctggaaca agccaaaact ctgggattct cccacgtggt
     1981 ctgtgaaaac gtttcccata tccggctgtg ccgatcgtta gggctgacgc ttcacggcgg
     2041 catgggactg aatgcggcaa atccgtcctg tctgtctttt ttgcagcggg aatccttgca
     2101 ggacgtgatc ctctcaccag aactgaccat tgcccagagc cgacactgca ccggactgcc
     2161 cgcaggagcg tatgcctacg ggttccagcc ggtcatgacc atgcggaact gtcccgtaca
     2221 ggcagaagtg ggctgccgga actgcaccca tggtctgaca gaccgcaccg gcagaacttt
     2281 tccggtatac tgccacaagg cagccggtat cacaacgatg tacaacgccg ttcccacgtg
     2341 gatggcggac aagcaggaac agctgtcaca cagcagcttt ctggtgctgg actgtaccct
     2401 gcagcccgat gcctgttccg tgctccgtgc ctaccagaac ggcgaaccta caaaagaagc
     2461 aatgacacgg ggactgtttt tccgtggtgt tgaataggag gcttttatgt atatttctgt
     2521 tcagaagctg cgtccgcacg cccagattcc caagcgtgcc accgcacaaa gtgccggcta
     2581 tgatctgtac gccctgccgg aggaaccgct gaccattcag ccgcaggaga tctgcaaggt
     2641 gcctaccggc atcgctgtgg ctccgtcccg tccggacgtt gccctgtgca tcttcccccg
     2701 ctccggactt tccaccaagc acggcatcac gctgatcaac agtgtggggc tggtggacag
     2761 cgactatcgg ggtgaactgc tgatcccgct gatcaaccac ggctcggagc cctttaccgt
     2821 agaagcaggc atgcggatcg cacagctggt cgtgcttccg atcctgacac cggaactgaa
     2881 ggaagtcacc cgtctggaca ccaccgaacg gggtgccggc ggcttcggtg caagcggact
     2941 gtcataattt cagaaaggag gacttcagaa gccctatgaa aaagtttctt gtgtatctct
     3001 gttccattgt ggtcgccgtg attctcggcg gtgtcatcgg caatctctgt gtggggatcg
     3061 ctcccctgag ctggctggac tacgccccgc cgctgcacct caaaactcac gagtggaatc
     3121 tgtatgtggc agacctgacc ttcggcattc agtcgaaatt taacatcgcc cagctgctgc
     3181 tgacgatcat tgcctgcttt gtcgctccca aaatcgagcc tttgatcggc aaaaagaaat
     3241 aaggcaatac cgcacaaaga gcgtctggcg actttgcacg gtattgccat aagacagcat
     3301 tggtttgact atttggtaca tttgctgtaa tttttacatc gtttttcgtt tgattttcac
     3361 aaaatgatag aatttcgatt tcgcagaaaa ttctgaaaaa aatgattgcc accccgcttt
     3421 tttcgtggta taatagaata cggtaacacc gcacaaagag cgtctgacga c
//
LOCUS       DAWBGR010000220         3391 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_6445_length_3391_cov_4.922662, whole genome shotgun sequence.
ACCESSION   DAWBGR010000220 DAWBGR010000000
VERSION     DAWBGR010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6445_length_3391_cov_4.922662"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          793 a          898 c          803 g          897 t
ORIGIN      
        1 aaatttgcaa gcagatggcg tgcaaagtcg ccagacgctc tttgtgcggt attgtcttat
       61 gcctttacct ttacgcccag cttgttgctt tccttgtacg gacgaaccag caggtagatc
      121 attgcaacga tcagtgcgaa tgcagcgatc aggctgatga tgtttacaac accgttgccg
      181 gcagtcatca gctgaccggt gaacaggttg cccagctggt tgacgatcag ggcaatgaca
      241 tatgcaaagc cgcattcgta accaattgcg aaccagaacc acttggtgtt gttcatctca
      301 cgcttgatcg cacccattgc agcgaagcac ggtgcacaca gcaggttgaa gatcaggaag
      361 gacatacctg ctacgccggt gaatgcagct gccagattgc tgtacacgcc gtcgccgccg
      421 ccgtacagga tacccatagt tgcaacgatg ttttcctttg cgaccagacc ggttacagcg
      481 gcaaccgttg cctgccagtc accccagccc agcggagcga agatccagca gatgccctta
      541 ccgatgtaag ccagaatgct gtggctcatc tggtcttcac tcagcatctg gaatgcaccg
      601 tcaacccagc cgaagtaaga agtgaaccag acgaagatgg tggacagtgt gatgatggta
      661 ccagccttct taatgaagga ccagccgcgt tcccacatgg agcggagcac gttgccgaca
      721 gtcggcatgt ggtatgccgg cagttccata acgaacggtg ccggatcgcc ggcgaacatc
      781 tttgtctttt tcagaatgat accggaaaca acgatcgctg ccatacccag gaagtaagca
      841 ccggttgcga tccatgcaga accgccgaag attgcaccgg cgatcattgc gataaacggc
      901 tgttttgcac cgcaggggat aaaggtggtt gtcataacag tcatacggcg gtcacgttcg
      961 ttttcaatgg ttctcgaagc catgataccc ggaatgccgc agcctgtgcc gaccagaatc
     1021 gggataaagg acttaccgga cagaccaaac ttccggaaga tacggtccat aacgaatgcg
     1081 atacgagcca tgtaaccgca ggcttccaga attgccagca gaatgaacag taccagcatc
     1141 tgcggtacga agcccagaac tgcaccaaca ccggcaacga taccgtccag aatcagaccc
     1201 ttcagccagt ccacgcagtt gacagcgtcc agtccctttt cgatcagtac cggaataccc
     1261 ggaacccata cgccataatc tgccggatcc ggttcaccct ttgcgaattc gtctgccgca
     1321 gtggtcaggt cgtccatgga agcggtttcg gtggtaactt ccagtgtttc ttcatcttct
     1381 acatcatagg agaagtctgc gttgtaatcc ttttcatagt tcaggatcat gtcctttgca
     1441 gcttcgccgt caaaggtgtc tgcttctgcg tcaatgtagc tttccagttc ttctgtatca
     1501 acgccctgtt cgcccagata agccacatag ccgtcaatga tcgcattggt gtcgccgtat
     1561 tcatcagcgg catcgttgta ggagctggtg ccgatgccca gcaggtgcca gccgtcgccg
     1621 aacagtccgt cgtttgccca gtctgttgca gcggaaccta cggtaaccat ggaaatatag
     1681 tacaccagga acatgatgac tgcgaagatc ggcagaccca gccaacggtt ggtgacgatc
     1741 ttatcgatct tgtcagaagt ggtcatgccg gttctctttt tcttcaggca gtccttgatg
     1801 atcgaagcaa tgtacagata ccgttcgttg gtgatgatgc tttctgcatc gtcgtccatt
     1861 tccttttctg cggcttcgat gtcttgttca atgtgctttt tggtagcttc cggaatgttc
     1921 agctgttcca gaaccttgtc gtcccgttcg aagatcttga tcgcatacca tctctgctgt
     1981 gcttccggac ggtcatgcag acaagcttct tcaatatgtg cgatggcatg ctccacgcag
     2041 ccgcagaact tgtgctgggg aaccatgggg atcttgcttt ccgcagcctg catggcttcc
     2101 tttgcagctt cgtcaatgcc ggtgcccttc agtgcggaaa tttccacgac cttgcagccc
     2161 agttcctttg ccagctggtc aatgcggatc tggtcgccgt tcttccgaac gatgtccatc
     2221 atgttgatcg cgacaactac cggaatgccc agttctacca gctgtgtggt cagatacagg
     2281 ttacgttcca gattggtgcc gtcgatgatg ttcaggattg catccggacg ctcctgaatc
     2341 agataatttc gggcaacgac ttcttccagt gtgtacgggg aaagagaata gatacccggc
     2401 aagtctgtga tgacaacatc tttgtgccct ttcagcttac cctctttctt ttccacggta
     2461 acacccggcc agttaccgac gaactggttc gaacctgtta atgcattgaa cagtgtggtc
     2521 ttaccagcgt tcgggttacc ggctaatgcg attttaatac ccatttcact ccatcctttc
     2581 gtgttagtgt ttactaatta gcaattacta acttgtgact aaaaaaataa tttcgccgta
     2641 aaatgaagat gtgaatccgc cgatgcagga cagattcacg gattttcttc tacagcatgc
     2701 acaacgatct gtgcggtgct tattctactt cgatcagttc agcgtctgct ttccgcagag
     2761 agagttcata gcctcttaca gtgatctcta tcggatcacc cagaggtgcc accttgcgga
     2821 tatagacctc gacacccttg gtgatgccca tatccatgat tctgcgtttt actgcacctt
     2881 cgccggtgag cttcttgaca cggacggtct gtccgatctt ggcatctttt aatgtagcca
     2941 tactccagtt cctccctctt tatatcataa tttttcctgc catttctttc gtaatggcaa
     3001 tacgggttcc ttttacattc acgataagat ttccgtttcc ggaatttact accgtgagcg
     3061 ttccgccgac aacaaagccg aggtcttcca agtgcttttt ggtttcagca cttccgccta
     3121 cttttttgat gattacttcg tcaccgggat tcgcaaacat cagcggcatc atagcatgct
     3181 ctcctcctta tatcagaacc gttcctctgt ttctcagagc cggctctgga attagaaaaa
     3241 gctaaccatt tgagaaatga taaaggttag aatcttctaa ccctcttgta agtatacaac
     3301 ttctcatggc atttgtcaag ggcaaaatgg agattttcaa attttgtgag attgcacaaa
     3361 taaaaaatct gaaagagaaa atgcattggg c
//
LOCUS       DAWBGR010000221         3370 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_6508_length_3370_cov_4.975566, whole genome shotgun sequence.
ACCESSION   DAWBGR010000221 DAWBGR010000000
VERSION     DAWBGR010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6508_length_3370_cov_4.975566"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          598 a          932 c         1036 g          804 t
ORIGIN      
        1 acgaaaaatt tgacgacgca tctgtcgcac aatctttgtg cggtattgcc ttggcttttc
       61 cctgcgacag cgtttccttt atgctcccag gatcttcttg gtcatgccgc ccaggatctc
      121 gatgcccttg acgatctgtt catcagacgg cgtggagaag ttcaggcgga acgacgtggt
      181 cggggcagtt tcgtccacca gaaaggcgtt tccggggaca acggcaatgg agtactcctg
      241 cactgccttg gtgcagaacg ccggcatgtc gcagtcgtcc ggcagggtgc accacaggaa
      301 cagaccgccc tgcggcattt cccactgtac cttgccggag aagtgacggc gaatgccgtc
      361 tgccatgagg ttgcacttcc ggcggtatac cgcacgcagt ttttccagat aggtgtccat
      421 gtccgtccgt tccagaaatt ccgcacacac tgcctgtgcc cagatgttgg tgtgcacatc
      481 ggcaacctgc ttgcagacgg tgatcttggc gatgatctcc ttgggggcac tgacatagcc
      541 cacacgcagt cctggtgcca gaatcttgga gaaggtgccg gagtagatca cgcggtgttc
      601 ggtgtccatg ctcttaatgg agggcacgtc gctgccggcg aagcggaggt caccgtaggg
      661 gttgtcctcc agaatgatga agtcgtactt gcatgccagc tcgtacagcc cctttcgctt
      721 ttccagcgac atcactctgc cggtggggtt ctggaaattg ggaatgacat agaccagctt
      781 cgggctcttg cttgccttga cggcttcttc cattgctgcc aagtccatgc cgtcctgttc
      841 catgggaatg cccaccagat cgacaccgta ggaacggaac gcgttcaggg agccgataaa
      901 gctgggggct tcgcagaaca gggtgtcacc gcggttcagc agcactttgc aggaaagctc
      961 atttgcctgc tgggcaccgg aggtggtgat gaggtcgtct gcatctgcat cgaagcattg
     1021 ctgtgcagcc atacgctgtt tcagcagatc gcggagtttc gggtatccct ccgtgatgtt
     1081 atactgcaac gccagaatcg ggtctttttc gaacagttct gcggagatct tccgaatggt
     1141 gtctaccgga aacgcctccg gagcaggatt gccggcggca aacgagatca caccgggcat
     1201 gctggtgaat ttcagaattt cacgaatcgc agagggctgc aatgcgtcaa cattttctgc
     1261 aaatttgtat tgcatgggta cattcttcct ttcgggcagc gttgcggtca gatacagtat
     1321 ccgcctgctg cacaggattt tggagccaca gcaggctcct gttggacaat gccgcctttg
     1381 acggcattac ctctctatta taacacatat tttcgttttc ggcaacataa atttttctat
     1441 ccggtgcaaa aaattcggaa acgctggata atgagtttcg atagaatccc tccaccagct
     1501 tcgctggtcc ccctcctttt tcaagggagg ctgatttcta tagttttagc cattacatca
     1561 tactagtcgg cactgcacaa cgcgtttcca ttctcaggac aggagaaaaa gctatgtttc
     1621 gaaaacaatc gttctggaaa ggctctgccc tgctgctggc atctgccatg actgccaagc
     1681 tgctgggggc atgcttcaaa attccgctga ccaatgtgct gggcggcacc ggcatggggt
     1741 atttcagcac tgcctacggg ctgctgctgc cggtgtatgc tctgtctgtc accggactgt
     1801 ctgccgccgt ggcacagacg gtttcccgtg cggcggctct ggaacactgg tcggaagtcc
     1861 gccggatcca ccatgcggca cggcttgcct gcggggcact ggggctgctg ctcagcctgt
     1921 gcatgtttct gctggcggag ccgtttgcca cgcagatcgc cgcccagccc catgccctgt
     1981 tctctctgct tgccattgca ccggcggcac tgttcggctg cctgactgcc gtggaacggg
     2041 gctactggga aggcttgcag aacatgaccc ccacggcgtt gtcgcaggca ctggaagcgg
     2101 tggcgaagct gggcttcgga ctggcactgt gcatgtgggt gctccggaat ccggaggtcg
     2161 tctgccgctg ggtgccgccg gaggtgccgc tttcggcact ggctgccgcg ggagccgtgc
     2221 tgggtgtcac gctatctaca gtgctggggt gggtgtactt cctgttgcat ggtctgcggc
     2281 gggatacccg actgccccgc gatgaccgtc cggcggcacc tgtttcccag attttccggc
     2341 ggctgctcta cgtaatgatt ccggtggcac tgggctcgct ggtgacgaac ctgacctctc
     2401 tgctggatct ggtgaccatg atgcgttgcc ttggacgggt gcagatgttc cacggggcag
     2461 aactggcgga gagattcggg gcatttgccg cccagccgga ttttccggca tttgtctacg
     2521 gctctttcac cggcatggcg atcactgtgt tcaatctggt gccctctgtc accaacatgc
     2581 tggggaaaag tgctctgccc tgtgcggcgg cactgtgggc gaagcaggac tgccttggca
     2641 cagcggcaca tgttcgctct gtcaccggag ccgcggcggc gatggcgttt ccggcggcag
     2701 gcgggctgtg ggtgctggcg gagccggtga tgcatctgct ctacgccggc agaccggaag
     2761 aagctgccat tgccgcagaa gccctccgga cactgctccc cggcatggtc tgcctgtgcc
     2821 tgacatttcc cttgtgcagt gtgttacagg gcatcggcag ggcggggatc cctgtgctgt
     2881 gcatgctggc aggggtggcg gtgaagctgg cggggaatct gctgctgctg tcccgtccgg
     2941 cgttctgcat caccggtgcc ggtattgcca catcagcgtg ttatctggtg ctgctgctgc
     3001 tgattctgtg gcagctgcac cgctgtctgg gagtacggct gcacttgctg cggggactgt
     3061 tcccgccggc ggcagcggcg gcgtgctgta ccggtgcggc gtggttctgc catacctacg
     3121 cccagccgct gggcatgctg ctctcggcgg cagcggcttc cggtgtgggc tgtctggtgt
     3181 atctggcggt gtggggactg ctgatgcgtt gccggaaacg aaaggctctt atataatagt
     3241 agggcagcgt ggtgaagcgg caactggctc tgaaacgggg aatgcgggga cacaacacca
     3301 tggaaattaa ttttaaaaaa tatgttgatt taaattgtgc caaattatga agtttgacaa
     3361 gtgaggctcc
//
LOCUS       DAWBGR010000222         3278 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_6766_length_3278_cov_11.843624, whole genome shotgun sequence.
ACCESSION   DAWBGR010000222 DAWBGR010000000
VERSION     DAWBGR010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6766_length_3278_cov_11.843624"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          725 a          844 c          835 g          874 t
ORIGIN      
        1 ggataccacc gttccggtgg caacggatac cacccagact acagaaggca gcaacagcgg
       61 tacaactgaa acggttcctg tcggtgtcgt tctgtacggc gacgtcaatc tggacggccg
      121 tgtagatgtt accgactgtg ttctgctgaa caaggcagtt gccggttcgg tacagctgga
      181 caccgcagcg aacaagaatg cagactgcaa cggcaacggc gaaatcagct ctgatgacgc
      241 aactgttctg atgcagttca ttgtaaacct cgtcaagacg ctgccgtaca acggctgatc
      301 tcagaacctg tcttcacaat catttgcaaa ccatttcatg atatgacaaa aggcacagct
      361 ttttcggaag ctgtgccttt tcattatggc aataccgcac aaagattgtg cgacagatgc
      421 gtcgtcaaat ttttcgtcag atgaaacaaa aagcggagtg aatacttgat gtattcacga
      481 gcatttttgt gaaatatgac gggaaatttg caggcagatg gcgtgcaaag tcgccagacg
      541 ctctttgtgc ggtattgcct tttgtcaatt tttccgtcac tgctcttgca gcatttcgtc
      601 cagcagttcc gctgctgttt ccacgaaccg cacacagggg cgtaccttgt aatattgttc
      661 ggtacgcggc tgcgggtctt tctggagttt ctccggcagg gatcgcagca gctcccgaca
      721 gaccagcgtg tcatgcttct ggcgaaactg ctcagccagc tggcggatac gggcataatg
      781 agcctttttt ccgtcgtaat ccgcagggtc gtcatatccc atcagcatgc cggcaaccag
      841 aaacatgccg gtgacagcac cgcaggtttc ccgcagtccg cccatgccgc cgccgaaaga
      901 agatgccagc cgcgtcgctt ctgacaggga cagccccatt tcctcatgaa atgcccctac
      961 gaccgactgg gcacagttat agccctcccg aaagagatct ctcgcttttt ctgcgtgatt
     1021 catatgtata cacttccttt gccctagaaa acactgaaaa aagcgttggc acagtgtctg
     1081 caccaacgct tttcattata tcacagtgaa aagaatttgt caattttttg tggaattgct
     1141 gtctgcatca gcggtttctt cttctgcttc tgccttttcc agagcctcat acgcctgttc
     1201 gtagaagtat tcctgtgaat tgatcagcgg ttcctgtgcc agctctctgg tataggtgcc
     1261 gtccggctgc tgtacccgag ccttgacgtt gtccgtcatc atcacatcga acatatccag
     1321 aatgcggtgc ttgatgtctg catcataaat cggtgcggca acttccacac gtcgtctggt
     1381 gtttcgggtc atccagtccg cagaggcaat gtataccttg cagttttcgt gtgccccgaa
     1441 gatatagatg cgggtgtgct ccagaaaacg tcccacaatg ctgcgaacag agatattttc
     1501 tgtcagttcc ggaacgcctg cgatcaggca gctgatgccg cgaacgatca tttcgatttt
     1561 tacgccagcc tgtgaagcgg cgatcagttt ttcgatcagg tatttgtctg tcagcgagtt
     1621 gatcttgatg ccgatatagg caggcattcc ggatttcgcc agttccgtct gctcgtcgat
     1681 catatctgcc agcttgtttt gcaggcactt cggtgctacc agcagatgct cggtgtgctc
     1741 cacagtctgt cccagacaga gtgcatggaa tacccgattg gcttccagac cgatgtcgtg
     1801 gttggcggtc atcagggaat agtctgtgta aatggcagag gtcttttcgt tatagttgcc
     1861 ggtacccacc tgtgtgatat aggaaatttc cgtacccact tttttggtga tcaggcaaag
     1921 cttgctgtgt actttcaggt tgtccagtcc gtagatgatg cggcagccgg catcttccag
     1981 acggcgggac cattcaatgt tgttttcctc gtcgaaccga gcccgcagtt ctaccagaac
     2041 cactacgtcc ttgccgtttt ctgccgcttc gatcagggct tctacaattt tactgttgga
     2101 agcaacgcgg tacagggtca ttttaatgga aacgacctgc ggatctctgc cggcttcgtt
     2161 cagcagggtg aggaacgggc ggatgctttc gtaggggaat gccagaaaac ggtcctgttc
     2221 ctctacttgt tcgatcatgc gtttggatat atcccgccat gccggcttct gcggaacccg
     2281 tcgctgatag aacaggcact tgttgtggcg gagcatatcc cgtaccaccg acagtacaga
     2341 gagattcagc ggagcttccg agtagaatac ctgttcttct gtcagattca ggtagttaca
     2401 cagtgagtga atgacagcag tgtccatcag gcgggagaat tccagcttga tcgggcaaag
     2461 ctttttccgg tcacgaatca actgttccat tgcttcgcgg tagtcgcatt cttcttccat
     2521 gcgttcgtcc ggttcaatgt ctgcgttgcg aatgatccgg atcagggact tgctcttgat
     2581 agtgtggcgg gagaaaatct ccggtgcaaa gtgcaggatc agttcttctg acagaatgaa
     2641 ccggttcttg ccggtgggca gtgaaacgat tttcggaaat acgccgctgc tgcacggcac
     2701 aaggcccagc ttttctgttc ctttcttttc cagtaccaca acggcataga tgttcttgtt
     2761 ctggagaaac gggaagggct gctttttgcc gatgatctgg ggggacagca gcggctcgat
     2821 ctctgccttg aaataggctt ccagccatgc cgcctcctct gtggtaatgg tgtggaaatc
     2881 tacaatgtcc acgccctgca tgctcagttc tgcacgcagg tcggaataag catcgtcctt
     2941 gtcacggagc agttcccgca cccgtgcata gatcgcagtc agctgttcct tacaggtcat
     3001 attggttttg ttttcgcgaa agtgggggtc ttcgcacatc tggtcgtaca aagaaccgca
     3061 gcgtaccatg aaaaattcgt ccagattgct ctggaaaatg gagagaaacg acagccgttc
     3121 acacagggga ttggcttcgt ccctggcttc ttccaatacc cgctgattaa atttcagcca
     3181 ggaaagttct cgattgtcat aacagtttgc gttcattaca atccctccga ttgcagttat
     3241 tcagtaatgt attagggcaa taccgcacaa agagcgtc
//
LOCUS       DAWBGR010000223         3251 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_6841_length_3251_cov_8.815081, whole genome shotgun sequence.
ACCESSION   DAWBGR010000223 DAWBGR010000000
VERSION     DAWBGR010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6841_length_3251_cov_8.815081"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1904..1913
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          752 a          846 c          736 g          907 t
ORIGIN      
        1 ttgcaagcag atggcgtgca aagtcgccag acgctctttg tgcggtattg cctcagaaaa
       61 agcgattatc cccagacttc ttctgcgatt tcctttacca gtgcgagctt tgcccactcg
      121 tctgcttctg tcagcttgtt gccgatctcg caggaagcga atccgcactg cgggctcagg
      181 caaaggcgat ccagcggaat atactttgct gcttcgtgaa tccgtgcaat aactgtctgc
      241 ttgtcctcca gttccggctt cttggaagtg atcagaccga gaaccacctt tttattcgga
      301 gaaacctttg caagcggttc gaagccgccg cttctttcgt catcatattc cagcagcagg
      361 gcatctacat tttcctgtgc aaaaacatag tctgcaacag aatcatacgg tccgctggtg
      421 aagaatgtgg agtgatagtt gccgcggcaa atgtgagaag tgatcaccat gtcagccggc
      481 ttgttctcca gtgccagatt gttgaccgcg agaagctgct tcttgacatc ttccagatca
      541 atgcccaagg actgataccg ctgttttgca gcatttccca cgatcgcacc ccaagtgcag
      601 tcgtccagct gcaaattgcg gcagcctgca tcatagaact gacggatcac ttcctgatat
      661 gcaacgccga tgtcgtggat cagttcttca ttggtggcat agaattctct ggtgctctgg
      721 aaattctgcg gcacgatcat ctgctggaac atctgtgccg gtgcaggaat ggtatacttt
      781 gcaacggttt catcatcttc aaactgtttc aggaactgga agtatgtcac aaacgggtgc
      841 ggcactacct tgacctttcc ggtcaggtag gtatcttcca gatctgccat ttctccgttg
      901 aactgcacac cgccgccggt cttttcatgt ccaacgccgg caaagcccca cataaaatcc
      961 agatgccagt attcacggcg atattcaccg tctgtgatga tgtgatagcc tgcctgcttc
     1021 tgcttggcaa ccagttcacg aatcgcagca tcttctacag cggtcagctg ttccttgctg
     1081 atttttcctg ctctgtaatc ttttcttgcc tgcttcaggt tttccggacg caggaagctt
     1141 ccgacaaaat catagcggta cggagatgtg atcttctgca tagtcaattc ctctttctga
     1201 acaaatgtat ttgattttag tataccatga aacagcaggt ttgggaaata tatttataga
     1261 gcagttgcct atagctttaa actatatcga aaaatctctt tcagttttta aggaaaatct
     1321 gcaagcggat tacgtgcaaa gccgtcaggc gggctttgtg cggtgttgcc ttaacttccg
     1381 taaaataaat ttgcaatgtc accttcccaa ctgtagttcc ggtaggtgcc atagtccgtg
     1441 attttgaatt ttctggtagc cgtgtaggtg ttgcctaccc agctgtaagt ataccgataa
     1501 gtgccctcca ttccggaaac tgatgcatca acactagtca ggttccagtc gtaactggaa
     1561 ccgccgtact tgtcggcaaa cacgttggct gcttcaattg ccagttccga attgccgttg
     1621 cagggaatgt tgtaggaatt ggttttctga tacttggacg cttccgtcgg cggctgggta
     1681 atgacttcgg aatattcgga gcttccggaa tttccggtac ttttttcgga atcgtcgccg
     1741 gaagaaccga tcaccgccgc gagaaccacg accaccagaa cggctgccac cgccagaatg
     1801 atccacttct ttttggactt tttctttttc acaggactct ccgcctctgc cgcaggaact
     1861 gcctcgtcca gctttttgcc gcacttctca cagaatttgg aatnnnnnnn nnnaattgtc
     1921 ctcaatctca cttccacagt atttgcaaaa cattgttcaa acttcccttc ctctttttct
     1981 gttgtggata actttatttt agcacaattg cagaagtctg aaaatacaaa acagttccga
     2041 aatttgccgg aaatttcgga actgttttca gttgacaatg tttcttcctc aggtgctgcc
     2101 cttattttcc cagaattgtc tgaaaaaacg attgcagcgg tagtgtcgca ccggaatgcc
     2161 tgccattcgg cacctgcaca agatctgcat cgcacagcag ccgtttcgta aacgcgtgat
     2221 ctccgatcac ttcgtcttct gccccgatga tacagtgcac cagactgctg tcgattgcag
     2281 caagcttgct gaacagcacc atcagcggct gtattggctg cgtatttccg agccttggca
     2341 gtacgtcaaa cggcatcaga caggggttca ccaagatggt gggaagatgc agttccgcac
     2401 agagcagtgc cgcaaaaaat ccgccaaagc ttgtccctat caaagtatcc gcgtgacatg
     2461 tcacagccat ttcccgcagt gttttcagca ctttctccgg acacactgca tcataatcca
     2521 gctgcggcga ggtcacatcg cagcccagcg aaagcaatgc tgtataggca ctgttctgtg
     2581 cactgccgcc gtagccatgt atgttgagaa tcattttttg gtttctcctc gtctttcaaa
     2641 aaatgtgcag ttgttccaag gttcttttgt cctcgacgcc gcgatagaat ttgtacagca
     2701 cacgggaaaa caagtcgtcg tccggtgcag cctgccggaa cagtgtcatg caggaacgca
     2761 ccttgcgtgc atctgtttgc ccgaacacgc tccacgggtt attggaggga atctccagca
     2821 gcaccgccgt gatctcccgc agccttgccc ccagaatcgg gtgctgatag tacgctaccg
     2881 cctcttccag atcgtaaatg gaaaaataca ccgcgataaa acttttcccc agcccctgaa
     2941 tctgcgggaa cacgtaccag atccagtgcg tcattttttg cccctgtttc atctcgtcaa
     3001 gtgccttggc ataggtttct ccaaattcgt acccgtgatc ctgtgcatct acaaaccgct
     3061 gcaacagttc ctgatttatg ttttgtggca taaagcatca cctttctttt ttatggcaat
     3121 accgcacaaa gattgtgcga cagatgcgtc gtcaaatttt tcgtcagatg aaacaaaaag
     3181 cggagtgaat acttgctgta ttcacgagca tttttgtgaa atatgagagt ctgtttcgta
     3241 tctgacgtat g
//
LOCUS       DAWBGR010000224         3203 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_6963_length_3203_cov_9.758577, whole genome shotgun sequence.
ACCESSION   DAWBGR010000224 DAWBGR010000000
VERSION     DAWBGR010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6963_length_3203_cov_9.758577"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          767 a          829 c          651 g          956 t
ORIGIN      
        1 gcagacggtc ggtctgggtg caggggctga tgctctgcac agtacaaacc gtctgtcaga
       61 ttgggacagc ctgggtgctt gcagggcttc ttacatttct tcggcacagc agtcacagcc
      121 tttgcaacta tcttctgttt ctgcagagag ttttttcaat gctttttggt attgttcctt
      181 cacccaggca acgctgtcat tcagttcatc tgcaatggca tcccatgttg cagcgtaaag
      241 ataccgcaaa cgaaggatct cacgctggtc ggcattgtga tttgccatga taagttcttc
      301 cagcttccgt ttcaaccgaa ttgatgcaat cagatcgtcc cacgctgcct ccacgatctc
      361 atgtatttca tcttcatcga tttccatcgc catagctttc caatcctgat aaatcacact
      421 ctgttccttg atgcgtctgt ttagatccat actgtttctt aaaacttctt ttgcaagcat
      481 atcgattctc ctttatggac acgaaaaaca gccctcgcag aatttcttcc gcaaaggctg
      541 tttcgctttc tcctgttttc ctactttaca gtataccaca tatgcgaact atcatcaagt
      601 gttatgaact atcatgaact atcaactttt catccctgcc aaggcttccc ggtgcaaacg
      661 ataacaggaa ggtttactgt atcccatttc ttctgcgatc tgattccagt ccttgaattc
      721 cagataacgc tttgccagaa tatcatgatg ctccgtatct gtgacggctt ttatcgcagt
      781 atcaaaaacg gctttcagat cttccagttc cttttttgca gtctttactt cttcctccaa
      841 ggataagatc tgagaaacgc cgctttccac ggcgtgagat tccggcgata cgggtttggg
      901 caaatcagaa taggcaggtg atttgggaaa agaaagtttc tgacgaagag catctgcttc
      961 cttttgtttt cggtcaatcc ttctaagaag tctttgtgcc tgtttcatgt attcttttgc
     1021 tgtcatgccg tgatctcctc cagcattctt tttacctcct ccacagaacg gacgatggca
     1081 gcgtttccgc cgcatttttg tattttgcga agagccgatt cctgcaaagc agttgctttc
     1141 cctttctccg tttttacttc aaaggcaaag aacctgccgc caatgcaggc gatcacatcg
     1201 gggattcctg ccgttccata catcccgcca tgctccttcc agcaaaaaca attcggcacg
     1261 gttttcagat acctcaaaat cgcccttacg atatccgctt ctttcaaact gctcacctct
     1321 tacctcttta ctgattttac agggaaattt ctattatact cataaaaaat gagaaaatat
     1381 atggggatat aaaataggaa atatataaaa gattacggga attccctgca aagcctgtaa
     1441 accctgtcag aacgctgtgc agacctctcc cctggcaagc tacacatggc tttctgaaaa
     1501 gctgatgcct ctccatgtcc tccgttttcc ggttctgtct gctgctttca cgaccgtggg
     1561 aaaatttgct tccagttcgt tgttgaaatt ctgctgactg tatggagcca tgccgcagct
     1621 gtcacagtat gctttatacc gtgcaaagaa ctccattctt cccacttctg catccatttg
     1681 caaagtacag cagtcccgaa caaacgccag cacactgttg ctgtcctccc ggtatttctg
     1741 gagttcctgt gcatttgcct gtgtttctga aaaatgaaaa tgattctgca tcagccgccg
     1801 cagtccttct aaggcaaatt gaaagatccc atctgcttca cagcggaact tctccagaag
     1861 ttccggatct cgcttgtcct ccggcacaga atgattgaac cggacaatga tcagacggcg
     1921 gtaaaagccc tccgatttgt ccccatagtt cttcggaatg ctgttgcagg aaaagagcag
     1981 cctcgcatag ggctgaaaag aaaagggatt tttgtttttc ttttccacag tcagataatc
     2041 ctctccgacc aacgccttga aaatgccgtt gtcttcaatg ccctttgtgg gcagctctgc
     2101 acagatattc gcccacttgc caaaaagttc tgcggtcttg aatcgatcat tcaatgcctg
     2161 ccatgctaca ttggacacat tttcttttcc cagcagcagt tcattcagca cccgcagcag
     2221 cacagacttc ccggcaccgc cttttcccac aatgataaag cacttctggg catgattgac
     2281 cggaatgaga aagtagccca gcatctcctg aatcagcgtc acctgatcct cctccacgga
     2341 ttcatgcaga aactgcagaa atctgggaca ctttgcatcg gacatatatc gcacattcag
     2401 ctgtaccgta gacagatact ttgcggtgtg ttccgataag gtttcgtcca gcacattgta
     2461 cagtccattt cgcacattga tgagataggg attgggattg agttcccgaa tatccttctg
     2521 tacctgcatc ttccattgtc cttcggtatc attgatctga gacagctttg tgtatctggt
     2581 cagcatttta tcccgtacca tatttcttgc tgtcagttcc gtaatgctgt gataaacgcc
     2641 attttcatag caataatact gctcggcaga ataaaacaca ggggcattct gtgtcatgta
     2701 ttctgcaagc acaccgggca gaaacttcga accccgttct gtcatttcat accagtcggg
     2761 aatttccatg ccggagcgat gcttccgtgt ttcggatttg ttttgaaatg ccttgtacag
     2821 ttctttttgc agagcaagca gcggcttgac atctgcattt ttgaaaccga aatgctgctt
     2881 taaatcgtaa tggatcatcg attcggcagt cacgctgtcc acattgtaaa gatattccga
     2941 cacaaagttt cgtgcagtct gcaaatcttc caccacggca ttttgcacct tttgctgcag
     3001 cagcagtgcc cgaatgccat caatggaaag cggctgaaag cacagagccg caggcgattt
     3061 acatttgcac tgtccgctgt tcagcttcgg gcaatgaaat cctttctctg caatagtacg
     3121 gcatgtcata ggcttggttc cgctgctgag aaaatgctgg atcttattct gcgtttcttc
     3181 aaaagaatac ttcggatacg gct
//
LOCUS       DAWBGR010000225         3200 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_6970_length_3200_cov_4.640700, whole genome shotgun sequence.
ACCESSION   DAWBGR010000225 DAWBGR010000000
VERSION     DAWBGR010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6970_length_3200_cov_4.640700"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          848 a          854 c          862 g          636 t
ORIGIN      
        1 taagcctccc ttgaaaaggg agggggaccg ccgctaggcg gtggagggat tctttcatcg
       61 ctacggcagc caaccgattt cacaaggagt tacgcttctt tttttcttta ctgaaaagaa
      121 aaaaagaagc aaaaaaagaa aagagttcgc attccacttg taccacgcgg caatgcacca
      181 cccgaagata tgacattttt attttaagat aagattaagc attctcccct ataatagaag
      241 ctggaaatct gtattctgaa aggaggattc tccatgaaaa ttcttgtagt agaagacgaa
      301 gtccagctgg cggatacact ggcggaaatt ctcaaacgca ataaatacaa cgtagatacc
      361 gttttcgacg gtgacgatgg tctggaatac gcccgcaccg gtatctacga ctgcatcttg
      421 ctggacatca tgctccctgt ccgcagcggc accgagatcg tgcagattct ccggcaggaa
      481 aaaaatgcga ccccgatcat gctcctgact gccaagtccg aagtggaaga caagattcac
      541 gggctggact gcggggcgga cgactacctc accaagccct tttccaccgg tgaactgctg
      601 gcacgaatcc gtgcccttac cagacgaaaa ggcgaagtgg aagtggattg cctgcacttc
      661 gcagaactcc agctgaaccg gcagacctat gacctgatac agggcgaaaa ccgcatgaag
      721 cttagcctga aagaatatca gatcatggaa atgctgctgt ccaaccccga acagatcatt
      781 cccaaggaac gctttatcga aaagatctgg ggctatgaaa gcgatgtcga atataacaat
      841 atcgaggtgt atatctcctt cctgcgaaaa aaactgaccg caattcactc cacagtgcag
      901 attcgcacgg ctcgcggcat cggctatttt ctggagaaaa aagcatgatc cggcgggtac
      961 ggcggcagtt tatctgcatt accatggcga tgctgacaac ggtgctcctg atcccgttga
     1021 ttgccctgaa tgtgattacc gaagccatga gctacaacca gacacggaac ctgctggagc
     1081 agatcgcgat cagtgaaact actgtgcggc agatgccgga cggtgctccg ccggacggaa
     1141 aacggccgca ggaaaccaca gacgctgttc cggctgccgg ctcggatact tccacggaaa
     1201 ctacagttac taccacagcc agcaccacta ctactgcgga aactaccacc gtccagacgg
     1261 aacgtgctcc tgcggaaacc aaggcggcag gaagcggtgc tccggctccg gcggcagtgc
     1321 agacacagcc acccacgccc cggcagacag ctgctcccaa ggcaacaacg gcagccccaa
     1381 gggcaaccac cgcacggcag accaaggctc ctgtcacgac taccaaggcg gttgtttcac
     1441 gtgaaacaaa gcccgcggca acacagccgc ctcagcctga tccccctcgt cagacagagc
     1501 cggatctgcc gcctgatccg ccgccgtggt ggggctggga cgatccgcct catgactacg
     1561 aggacaacag ggaaaattac gccgcccggc agccggtgct ctacgccatg ccgctggcaa
     1621 gtctggcaca gtctgtgccc atgaacaacg gcggtcggaa cgctccgccg tttccggaca
     1681 aacggggcga cgtggtgacg atcgaccact tcctctgttt tgccaacgcc gagggcacgc
     1741 tgatgcggct ggacggtacg gaaaactaca ccgaggacga tgcacagaca atgctggact
     1801 acgtgctgga aaagggaaag acagacggct ggtacggcag cctgcaatat ttccgcaagg
     1861 attacgacag gggaacgctg gtagtatttt ccgaccgcag tgcggaacag ctgctgctgc
     1921 acaaggtgct gctggtcagc attctggtgt tcctgctgat ggagggcgtg gtattttttc
     1981 tgaccatgat cctgaccaaa cgagccatgc gtcccatgca ggaaaccttt gaacgccagc
     2041 ggcagtttat ctccgatgcg gggcacgaac tgaaaacgcc cttgacgatc atttccgcca
     2101 atgtggacat tctccacgac gagatcgggg agaacaaatg gctcagctat atccagtcgc
     2161 aggcggagcg aatgcgggtg ctggtgggcg agatgatgaa cctgaccaag ctggaaatgg
     2221 gggacaaggg aaaagatttc gtggacttct ctttgagcga agccgtttcc ggtgcggcac
     2281 tgccctttga ggggcaggcg ttcgagcagg aaaagcagct ggaactggag atacaggagg
     2341 acatttccta ccacggcaac cccgaccaga tcaaacagct ggtgggtatc tttatggaca
     2401 acgccctgaa atacagcaag gaacacggag aaattcgggt gacgctgcaa caggtacaga
     2461 acaagaaaac gctgaaggtc tataacaccg gcaagggcat tccggagtcg gaaaaggaga
     2521 agatcttcca gcggttctac cgcagcgacg cgtcccgtgc cagagccacc ggcggttatg
     2581 gtctggggct gtctatcgcc cagagcattg ccgatgccca taagattcgc atacaggtgg
     2641 aatcggaata cgaacactgg atatgcttta ttctgacatt ttagatgacg ttttagaaaa
     2701 taaatcggat atgcaaaaat ccctctgcga gtgcaggggg atttttttac ttttgcagca
     2761 ttggatttta cgaaagaacc tcttggaatg tgtaggggaa tgcccacatc gccccgcaga
     2821 cttgtgcata agcctcccct gaaaggggag ggggaccagc gaagctggtg gaggggttct
     2881 gctgaaatct tttactcctg ttttaggttt cacggaagaa acccctcagt caagcctgcg
     2941 gcttgccagc tcttaccgag ctgtcccctt tgtcacttcg tgacatttcc ccattccatg
     3001 gggaatcacc cttttcaagg gagcctaagt acaaatgagc ctcccttgaa aagggagggg
     3061 gaccgccact aggcggtgga gggattctcc gccacagcag ccaataggtt tcataagaag
     3121 caaaaaaaga aaagagttcg cattccactt gtactgtaag attccaatat cacacgaaga
     3181 ttacacagag aatcttccac
//
LOCUS       DAWBGR010000226         3181 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_7018_length_3181_cov_6.865963, whole genome shotgun sequence.
ACCESSION   DAWBGR010000226 DAWBGR010000000
VERSION     DAWBGR010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7018_length_3181_cov_6.865963"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          932 a          702 c          839 g          708 t
ORIGIN      
        1 cagaaggttg ccattcgtgc caatacacct ctgttcgcca gtgcagaaac tacatctgta
       61 accagaagaa tcagcggtac ttactatctg tatgatggca ttgcctgcaa gaacggtcgt
      121 tatcgtatca ccacaaagcc ggagttctgc ggaaagacac cagtgggacg atttgtgact
      181 ggttatgttt cttgggataa tttcggggtg atcggatgaa tgcagaacaa aaagaccaga
      241 tccggcagct gcacagcagc ggtctgggct acaagaaaat cgcagcccaa ttagggctgt
      301 ccgtcaacac cgtagcttct ttctgcaaac ggcagagaga aagcgaatcc tgcccacact
      361 gtccgcagtg tgggcgttct gttgtgcaga caccgcaccg aaagccgaaa cgattctgtt
      421 ccacacaatg ccacaatact tggtggaatc accatgctgt atcgaggaac ggcaaattac
      481 agcagctctg ccctatttgc aaagagccgt tttttgccta tcccagttca caccgaaaat
      541 attgttcccg tctttgctat gggaagtaca gaaaggaaat gtgtcatgga aaaagaacat
      601 taccataaga tcattacgta tcaaaccaca gtttcgattt tgaaaagctg gatgcatgct
      661 ggattggtca cgccggagga attccaaaaa atcaacacca taattgccga acgttccggc
      721 atatctttgt gcagtatatt ccttgactcc tgcccgatcg tacggtaata tgtcatcgga
      781 aagggggaga ttatcacggc acgagtgata caaaaagttg catttccaca gaaaaagccg
      841 ttcctgttga aacggacggc agcctatgcc agagtgtcca gcggaaagga tgccatgctc
      901 cattctctgt cggcacaggt cagctattac aatcagctga tccagagcaa tccgaagtgg
      961 ctgttctgcg gtgtttatgc agatgaggca ttgacgggaa caaaggaaaa tcgtgcagaa
     1021 tttcaaaagc tgctgaatcg atgccggcgg ggagaaattg acttgattct gacaaagtcc
     1081 atttcccgtt ttgcacgaaa cacggtcacc ctgctggaaa cggtacggga actgaaaaca
     1141 ctgggcgttg atgtctattt cgaggaacag cggattcatt ccatgagttc agacggcgag
     1201 ctgatgcttt ccattctggc atcttacgca caggaggaaa gctattctgc cagcgagaac
     1261 aaaaagtggc agatgcgaaa ggactttgaa cagggaaaag tcgggagtat gcgaatgctg
     1321 ggctatcggc gaaccaagtc cggaaaacta gaaatcgtac cggaggaggc agaaatcgtt
     1381 agaatgattt ttctatatta tctgtctggt atgggcaagc tggcaattgc caagaaactg
     1441 aatgaacagc agatatgcac ggtgcgtggc tgtgcatgga cgacagagga cgtaaggcga
     1501 acgctccgca atgaaaagta caccggaaat ctgttgctgc aaaaaagttt tcgggaaaat
     1561 cacattacca agaaaaaggt ggctaacatc ggacagcttc cgcagtattt tgttgccggt
     1621 tcgcatgaag ccatcatttc gcaggaacag tttgatgcgg tgcagaaaca aatggcggaa
     1681 cggcagaaaa aatatgccgg ttcctgtacc acaaaccgat atccatttac gcagaaaata
     1741 cggtgtgcct gctgcggcaa gtattaccgc agaaaaacga ctgtgaccgg tgtggtctgg
     1801 atttgttcca cttacaacac caaagggaaa aaatactgtc caacggcaaa acagattccg
     1861 gaaaatacgc tgatttctgc ctgctgtgat gttttggaaa tatcggaatt tgatgcggag
     1921 cgatttgcgg aacaaatcga acagattcag attcctgcac ccaatgaact gcaattctgc
     1981 ttttcggacg gaacggaaca aaccgtatct tggaaagacc gttcccgttc ggaaagctgg
     2041 acggcggaaa tgcgagagaa agcgaggcag aaaaaatggc gacagtccta aaaataccgg
     2101 caaagtttca tcccataacg catttgccgg aaaccaaggt tcagaaacgc agagtggcag
     2161 cctatgccag agtttccaca gattccgagg aacagcagac ctcttatgct gcacaggtag
     2221 atcgctacac caagtacatt caggaacggg cagactggga gtttgttgcg gtctacaccg
     2281 atgagggcat ttctgccctg aataccaaac atcgggatgg tttcaatcgc atggtggcag
     2341 atgctctgga tggcaaaatc gatttgattg tcaccaagtc ggtcagccgg tttgcacgaa
     2401 acactgtaga ttctttgacg actgtgcgaa agctgaaaga aaaaggcgtg gaggtgtttt
     2461 ttgagaaaga aaacatctac acgctggatt ccaaaggcga gctgctgatc accatcatgt
     2521 ccagtctggc acaggaggag agccgttcta tttcggagaa tgtaacttgg ggacagcgaa
     2581 agcgaatggc ggacggcaag gtcagcttgc cgtacaagca ttttctgggc tatcgaaaag
     2641 gagcagatgg cttgccggaa attgtgccgg aggaggcgga gattgttcgg aacatctatc
     2701 gttggtttat ggaggggaaa acgccaactg gcattgcgag aacattgaca gaacagggcg
     2761 ttccgacacc tgccggaaag gaacaatggt gttccagtac agtgaaaagc atcctgacca
     2821 atgaaaaata caagggttct gctctattgc aaaagagatt tacggtggat tttctcacca
     2881 aaaaatctaa ggtgaatgag ggcgaagtac cccaatacta cattgaggaa agtcaccctg
     2941 ccatcatagt gccggaggaa tttgaactgg tgcaggcaga attgctgcgg aggcaaaacc
     3001 tgcggcgaca gtacaatgga aagagcgtat ttgctgcccg gcttgtctgc ggcgactgcg
     3061 gaaatttctt cggggcaaag gtctggcatt ccaacagcaa ataccggcag gtgatctggc
     3121 agtgcaatca taaattccaa ggggtgtgca aatgccagac accccatttg caggagagcg
     3181 t
//
LOCUS       DAWBGR010000227         3130 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_7143_length_3130_cov_4.745041, whole genome shotgun sequence.
ACCESSION   DAWBGR010000227 DAWBGR010000000
VERSION     DAWBGR010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7143_length_3130_cov_4.745041"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          774 a          832 c          728 g          796 t
ORIGIN      
        1 ttctgcctcc cttgaaaagg gagggggacc gtcgcagacg gtggagggat tctactgaaa
       61 tcttttcctt ttgtttcaga ttccacgaga taatccctca gtcagcctgc ggctgccagc
      121 tcagcacaag gcacgcccta cgggtgcctt ttcaagggag ccttttctgc accttgaatt
      181 ctgcctccct tgaaaaggga gggggaccgt cgcagacggt ggagggattc taccaaaatc
      241 ttttcctttt gtttcagatt tcacgagata atccctcagt cagcctgcgg ctgccagctc
      301 ccttttcaag ggagcctttt ttaccttata gtccacctcc acacaatctc ttcacaaatg
      361 tgaaaaaacc acgacagaat gacaaaaccg ccggaaacca aacaaagcac ccagcaccca
      421 atgtgctgaa tgctttgtta ttgtcttagt tacttaatta gatacttaag aaacgtctag
      481 attacaaata tgtgccaaca ttattgctca ttgtatatgt agatgttgta ttattacgag
      541 tataagtcaa accagtaacg cgtccatcgg catcaacagt aatgctctta acagtatcag
      601 ccggaacttc tgccaagctt tcaaccgcat ccaaatagtg attatcatca gtattagttt
      661 ccgctccctt gatggcatca aaactaatta caaggttacc aaccttatat gctccgctac
      721 catcagcata tgcaccagaa gcaatcgtct gtgccgcctg aacagcagac tggcactcac
      781 tacgcacact ctgttccttt gtcttcttaa tataaccagt caaagacgga atcagagccg
      841 cggccagaat tgccatgatg accagaacga cgatcagttc gacgagggag aagcccttgc
      901 ggttcttttt cttcttcatt gcagtgttga atacgttcat cggggttttc atattggttt
      961 ccacctttca aatttgaatt aagtaaaata aaataactgt gttcccgaaa tctccgcttt
     1021 cgcgtctagc cggcatcgcg tccggagttt caggagaatc aaacgagagt tcttatcaac
     1081 gttccctctc gttgtttgca agtttattat agcacaatct ggcggtgctg tcaatagtgt
     1141 ttgtgcatat tctgcatttg gaacaacaaa caagctcact ttttgtgcac atctacaaca
     1201 catcggaaaa gtggcagaat tgcgtggctt ttcctttcca ttttttagct gattctgtac
     1261 gattccgata gtgcggctct aattttgata aaatcgtgtc aagttttaca taaaactatt
     1321 ttgattctgg agatttctca aatgcagtcg attttccgcc tcaaaatgcg aacgaaaaat
     1381 tactcccaac ttttccgcga atttgataca ttttctctaa aaatcatgta acgcgttaca
     1441 tttcgcggga gttgagggga gaatgttgcg gggatttttt ataggcacct ttttctgcct
     1501 cccttgaaaa gaaaaagggg accgccgcag gcggtggagg gattttcact gaaatctttt
     1561 ccttttgctt cagatttcac gagataatcc ctcagtcagc ctacgggtgc ctttttctgc
     1621 ttcccttgaa tttgcctccc ttgaaaaggg agggggaccg ccacaggcgg tggagggatt
     1681 ttcactgaaa tcttttcctt ttgtttcaga tttcacgaga taatccctca gtcagcctac
     1741 ggctgccagc tcccttttca agggagcctt ttttctacac cttttctgcc gttctcccag
     1801 caaacgcatc acccattcgc cccacgcact gccaggaaca ccataaatcc cgccagcacg
     1861 actacagcca gcaccagcac cggcgagaac atcgcggtgc ggcttcgggt tgcctgcttc
     1921 tgggtgggat acggcagggg ctggcggcgt tcgtacagca gccagaacaa cacgcccatg
     1981 atcgaaaatg ccagtaccgc cataccaaac ggcaggattc ccacattggc gactttctcc
     2041 ggcagcttct cctgtagcag tgacagcacc atgctgttgg tattcactgc catgtgtacc
     2101 agtatggacg gcagcagaga gttatgccgt gccacgatct tccccagaaa cagccccatg
     2161 acgaagccca gaataaactg ctggaaattg ccgtgcatca gtccgaacag cagagcactg
     2221 gtgacgattc cgaagcggac gctgaccgcc gacaggcttt tcatcaggaa gccgcggaac
     2281 agcagttcct ctgtcaccgg agcaagcacg caggtgtaca gcaatgccag cagcatggac
     2341 tgggtgcttt ggaaatagtc gaaagatgct tccggcagtt ccagatgcag cagctttgtc
     2401 accgcgtaca gcaccacgga aagctgctgc aatgcgaagc cgatgcagat gtactgaaag
     2461 gctttccagc cggaaaaatc cgtcgtccgg aacagtccgc ccagcggctg gcggatacgg
     2521 cggcagccca gccatgccac cagcgtgttc attcccccga agatcaggca gtgcaacgcc
     2581 atgtagatgc ccgacgattc catggtgcgg atcgctccgg actggtcgcc gttgaagtag
     2641 gaagccgccg tgccgtccat gcacagcagc agcaccataa tcatcagcgg gaacaacagc
     2701 tggctgccca tacagcccag caacgcccct gctccggcgg tgttcatctg gcggcgaatg
     2761 cgggaacgct cctcccgttc cggaaacagc gggatatgaa agtccggcag caggatctgc
     2821 ggcagaaacc gccggtaatc cgtccggtat gcggcgtttt ccaatgcatt ttcaaaagga
     2881 ttctgcggat cgatctgccc cgcagtttcc tcggtatagg gttcgtttgt catgggttga
     2941 cctccttcgc gttaaggaaa gctgtcacgc agcttttcct gaggcaatac cgcacaatct
     3001 ttgtggggta ttgccctgaa tatacaacag tataccatat ttttcgggaa ttgtacagag
     3061 ggctttcaca gtattcacga aaatcaattt tcatgatagc ctcccctgaa aggggagggg
     3121 gaccgccgtt
//
LOCUS       DAWBGR010000228         3124 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_7159_length_3124_cov_4.458456, whole genome shotgun sequence.
ACCESSION   DAWBGR010000228 DAWBGR010000000
VERSION     DAWBGR010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7159_length_3124_cov_4.458456"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          759 a          853 c          843 g          669 t
ORIGIN      
        1 ggggtacttc tgtaaactct tttacaccaa gcaaaagatt ccacaaagaa cccctccacc
       61 gtctaacgac ggttcccctc ccctttcagg ggaggctttt ttaggctccc ttgaaagggg
      121 agctgtcacc gcaggtgact gaggggtact tctgtaaaac ctgatacatc aagcaaaaga
      181 tttcacagaa aaatccctcc accgtctgac gacggtcccc ctcccttttc aagagaggca
      241 attttatttt tcttgacatg tgggattttt ttctgaaaag ggcttgcttt tttttcggga
      301 atgtggtata atgtgaaaga taatgaatgt ttgcaggttt ctgcatcgtt cagcacgaag
      361 tcaccgctgt tatgctgccg ctttgtgtac tcgaaactag acgggaggag ggactttccc
      421 atgagcgtac tggaaatcat tgttggtgct gtggtactgc tgatcagcgt tgttctgatt
      481 attatctgca tgatgcagga gcagaagccg cagaacgcca ctgctgcact gactggtgca
      541 agcaacgatt cgttctatga caaaaaccgc ggacgcacaa aggaagcacg tctgaagaag
      601 atcacattca tctgctcagt cgttttcttt gttttgattc tgtttatgga tattgtaatg
      661 ccgctgctga ccaagtaaac ggcagatcgt cctgtgcttc aggagcctgt ttcgattcta
      721 ggttatctag gcggaacagg ctcttatgtg ttttccgtcc ggaacagcac aagtacagga
      781 cttttttctg actgcacatc ttctttctgc acattcggca atatcgtcag aaaaacaaca
      841 gcaatgagaa agccgcacag ggcggcagaa gtgaaaggat ttttatttta tggctaatac
      901 ccaactgggc ggggctgaaa cgatcgacag ctaccgcaag aagatcatga cgatcctgaa
      961 gaaaagcggc cgcacaccgg tcagcgaccg cgatctggct accaagtgcc gcaccaaccg
     1021 cggcggggct gccaatttcc gcaaggcggt gcaggaactc tgcaaagagg gtgcgatctg
     1081 tgaacgccgc cgcggctatg tttcctccgc aaacatgggc tactatccgg caacgatcgt
     1141 ccggctgagc cgcacattcg gctttgcccg tcccgaagaa gaaggggctc aggacgtgtt
     1201 tatccccggc aagttcttac agggtgccat gaccagggat cgcgtcctca tcggcaacat
     1261 tccctcccgc agcggcaagc ctgaaggcga agtgctggcg attctggagg aagcagacag
     1321 caagatgacc ggcgttatca ttgaggacaa cggcaagctg atgttccgtg cggactccat
     1381 gtccaagact cccattcaga tccagcgaaa ggacagcgtg atctatcacg agggcgaaaa
     1441 ggtgctggca gaagtggttt accgcggtca gcggcacgcc gagcacaagg tcaagatcgt
     1501 gttcagcttt ggtgtctgca cccgtgcaag cgtctgtgcc gaagcaatgg ttgccgttcg
     1561 cggtgtcaac gtggactttc cggaagaagt cgttgccgaa gccgaccagc tggcaaatgc
     1621 tcccattccg caggaggaat acgcaaagcg tctggacctg cgggaaaccc cgatcttcac
     1681 cattgacagt gccaagtcca aggacctgga cgatgccgtt tctctggaaa agaccgaaac
     1741 cggctaccgt ctgggcgtgc acatcgcaga cgtttcccac tacgtccgcc cgcacacccc
     1801 gctggacaac gaagcactgg atcgcggcac cagcatctac tatgcaaacc gcgttattcc
     1861 catgctgccc accagcctgt ccaacggcat ctgctccctg aacccctgcg aagaccgtct
     1921 ggcattctcc gccctgatgg acttggacga aaacggcgag atcgtcaagt acgagttccg
     1981 gaagaccgtg atccgttccg ctgtcaaggg cgtttacagc gaggtcaacg agatcctcgc
     2041 cggcacggct tctccagagc tgctggaaaa gtacaaggct gttcagcaca tgatcccgct
     2101 gatggacgaa ctgtgcgaca ttcgcctgcg ggaacggaaa cgccgcggtg ctccggaact
     2161 ggaaaccgca gagggtgcac tggtcatcaa tgacgagggt gtctgcgtgg acgttgtggc
     2221 acgggaacgg ggcaagagcg agtgcatcgt cgaggaaatg atgctgctgg caaacgaagc
     2281 tgccgcaaaa atggcacggg aaaagaacct gccctttgtc tatcgtgtgc acgacaagcc
     2341 ctctccggaa aagatcgagc gtctggaaca ggtcctgctg cggctgggtg tcgaagtgcc
     2401 ggtattcacc gacatcaagc cccgtcatct ggcagagatt ctggacaacg cacggggtac
     2461 tgaactgtat ccggttctga atgtcatggt gctgcgttcc atggcaaagg cagcctatgc
     2521 tccggaagcg atcggacact tcggtctggc actgaaagac tatgcccact tcacttcccc
     2581 gatccgccgc tatcccgatc tggcaattca ccggatcctc agcaactact gcgagaaccc
     2641 caacgccgaa gcgatgcacg aaaagtacga tgctttcgtg atcgaagctt ccatgcgttc
     2701 cagcgacacc gaagtacgtg ccattaccat tgaacgcaac gccgacgact gctacaaggc
     2761 agagtacatg cagcagcatc tgggcgaaat tttcgacggc atcattgccg gcgtgaccga
     2821 cttcggcttc tacgtggctc tgcccaacac cgtagaaggt ctggtacacg tttccaccct
     2881 gccggaaagc gactggatca acgacgacag cattgctttc aaggaagaac agggcagtgc
     2941 atgttaccgt ctgggcgaca ccgtgcgggt acagtgcacg aaaatcgatg tcaacagcgg
     3001 caacgtagac ttcgcccttg tagaagaagg acaggaagcc gccgccaagt aaaggcaatt
     3061 ttcaatctca ggcaataccg cacaaagagc gtctggcgac tttgcacgcc atctgcttgc
     3121 aaat
//
LOCUS       DAWBGR010000229         3092 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_7254_length_3092_cov_4.905499, whole genome shotgun sequence.
ACCESSION   DAWBGR010000229 DAWBGR010000000
VERSION     DAWBGR010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7254_length_3092_cov_4.905499"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          736 a          893 c          809 g          654 t
ORIGIN      
        1 agtcgccaga cgctctttgt gcggtattgc ctataggagc ctgctttcct tttgaaaaca
       61 ggctcctatc acatcatatt acaaaaaaag gaatttttca aggacatttc tgcggaacag
      121 atgcaacgat tctggtaaaa gttctcattc aaatacccga tactttttct cgttgtgtcc
      181 gtaggcactg accacaagcc ccagtgcaac gtacatgctc acagttgccg taccgccggc
      241 actgataaag ggcagcggca caccgataac cggagccacc ttcagcacca tgccgatatt
      301 catgacacag tggataaaca gcatacagaa cacaccggta caaaggaaca tgcccatttt
      361 gtccttggtg ttgaagccgt catagagcac tttcagacag aggaatatca gtaccagcac
      421 cacagcaact gcaccgacaa atcccagtgc ctgccccacc tgtgcaaaca taaagtcatt
      481 gtgaatttcc ggaacagaaa catagttttc cttgccggca tgcagcccca cgccgaaaat
      541 ttttccgttg gacagggctt ccagaccacg gtcctgctga tactcgatat tctccggatc
      601 tgtgccgggg tgcagcagca ccagaattcg ttttttatga gtgtccccca atatgaaatt
      661 ccacgcagcg accagtgcca gcggaatggc aatgagaaat gccagcacat atttccatga
      721 gatcttcgcc gcaaagagca tacccagaaa aaccaccgca aacacgatcg ccgtaccata
      781 gtccccctgc aaggcgatca gccccgtggg gatcgctccg tgaacacaca gcagcagcat
      841 gtgcaggggc ttgttcatgt tgctctcatc tttggcaagg tgcagggaaa atgtcaggat
      901 aaacgccagc ttcagcactt ccgagggctg gaactggata aagcccagat tcagccacgc
      961 ctggtcatcg gcaccggcac gcttgtgtcc caacgccgta aaggtcagca gcgtcagcac
     1021 cagtgccacc ggagcataca ggaaccagag ccggacgagt ttgtggtaat cgatcgccga
     1081 gagcaccagc aggcaagcca aacccagtcc gcaggagagc agctgcgtcc ggtaatagct
     1141 ggagcccaca gaactgataa tgtgattctg ccagatcgag tacagcagga taatgctcag
     1201 cacagaacag agcagtgctg ccagaatcag cggcttgtcc agccgccgcc agtagaccat
     1261 cagcgggctt actggttttt cacgtttgtt ggatttgtat gatcggttca tgaaatgtcc
     1321 tttctttcag gcaatatcta tcagccgtta gtgccccagt atttccgatc cagactgcgg
     1381 aactgggcgg ctgcggcaat gtgctgtttc cgaatgatct cgctgccgtc cagatcggca
     1441 atggttctgg caactttcag aatccggtca taggcacggg cactcagccc cagcttatcg
     1501 aacacagcac ggagcagtgc cgttgcggca tcgtccatgg gacaaaagtc ctgtagcatg
     1561 cctgccggaa tcgctgcgtt gcagaaaatg cccgttccgg caaaccgctg gttctgtagt
     1621 tcccgagccg tctgcacccg ctgccggatc gccgccgacg attccgcctt tgcctttgca
     1681 gagagagaat cgaacgacac cggctctact tcaatatgca gatcaaaccg atccagcagc
     1741 ggaccggaaa tgcggttcag atactgccgc acctgccgtt cggaacaggt acacttccgc
     1801 ttcgggtgcc cgtagtagcc gcagggacag gggttcatgg ctcccaccag cataaacgaa
     1861 cagggatagg aaaccgttcc cgccgcacgg gtgatggtaa ctttgcgatc ctccagcggc
     1921 tgccgcatga tctccagtgt ccgatggtcg aactctgcca gttcgtccag aaacagcagt
     1981 ccgttatggg caagggaaat ctcccccgga cggggcactg tcccgccgcc caccagaccg
     2041 gctccggaaa tggtgtggtg cggcgaacgg aacgggcggc gtgtcatcag cgggtgtttg
     2101 ccgtccagca agccggcaat ggaatagatc tgcgtggttt ccatggactc cgcggtagtc
     2161 attgccggca ggatcgacgg gatccgcttg gcaagcatgc tcttgccgct gccggggctg
     2221 ccgatcatca gggcattgtg cccgcctgct gcggcaattt ccaaggcgta ctttgcattc
     2281 tgctgtccct tgacatcggc aaagtccagc atttcctcgt actcctccgg ctggggcgta
     2341 tagggttcct gcggacgaat tgccggacca gtgtcctcgt tcaggtgggc aatcagttct
     2401 cccacgctgt gcacaccgta caccggtatt cctgccgcgg aagcttcaga agcattctcc
     2461 gccggcacat acaattccgt cacgccgttc tgggcggcaa gcagtgccat aggcagcaca
     2521 ccgctgacag aacgcaccag tccggacagg gaaacttccc cgataaaaca gcggctggcg
     2581 ggctgtgccg gaatccgttc catgcacacc agaatcccca gcagaatcgc cagatcgtgc
     2641 atggtgccga ttttcttcac gtccgccgga gccagattca ctgtgatcag gcgggcagga
     2701 agggcaatgg acaagctgga cagagccgaa cggattcgtt cccgactctc ccgcactgag
     2761 gtatccggca gaccgacaat ttccatttgc ggtttgccgc ggttcatgct gacttctaca
     2821 tctacagaaa aggcattcag cccgaacagt cctaaacttt tcagttgggt aaacaaaagg
     2881 gttcacctcg ttttttcaaa tgctgtcccg tcattgcggc ggaacagttc ttccggcagc
     2941 accgtgcaaa accggcatgc ggaactggtg cggtgctgcc tctatcagta tagcatgatt
     3001 cacaaaaaat gtaaagacaa tctgcaagaa agattttggg caacaccgca caaagcttgt
     3061 gcgtaaggtg cgttgtcaga tttttcggca ga
//
LOCUS       DAWBGR010000230         2999 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_7530_length_2999_cov_4.839674, whole genome shotgun sequence.
ACCESSION   DAWBGR010000230 DAWBGR010000000
VERSION     DAWBGR010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7530_length_2999_cov_4.839674"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          826 a          757 c          765 g          651 t
ORIGIN      
        1 tgcacgcaga tggcgtgcaa agtcgccaga cgctctttgt gcggtattgc cccagcttcc
       61 ctgcacatga caaaaatgaa aaattgaaag gaattcacat aaaatggcac agaaaaaaga
      121 acatgtacag acacattatc ttcccaccga aagctatctg gcaaccagag ccgctgctct
      181 gcactttccg gcaaagcagt ttgcggacat gattaacgac cagaacagcg tttacggcat
      241 ggtcattgat atgccgatca accccgttac cctgtccaca ctggtggtat acatcaacgg
      301 tgcggcaaac gtttacttca acaacggcgg ctcctacacc ggtgcttccc agcggtatca
      361 gaccgtggta caggccggac ggctgctggt ggcaaacgct ccgagaatcc tcggcgaatg
      421 cagtcagact acctctttcc cgctgcccac ttcccagccc caccacctgt atctgctgac
      481 caagaagggc attttcgaaa agaaagtcct gccctctgcc atcaaggaag aaagcgaaga
      541 catgcaggcg atctatttcc tctatcagca ggtcatgcgg gaacttcgtt ccgcacagct
      601 gaaagatcag gcaatgtccc agaagtaaac cggaacggag gtgtttcctg tgattccata
      661 cgaaaagaaa gaacactata ccattgacga tctggcggag atcgtgacgc tgctccggag
      721 caaggacggc tgtccgtggg acaaggtgca gacgcatgaa tccattcgtc cggacctgat
      781 cgaagaaacc tatgaagtca tcgaagccat tgaccagaac gaccccgaaa tgctgcggga
      841 ggaactgggc gatctgctgc tgcaggtggt gttccacagc cgtatcgaaa cggaacaggg
      901 acactttaca ctggacgatg tctgcacaga catctgcaac aagctgatta tccggcaccc
      961 tcacgtgttc agcacggtgc aggcggacac cactgaagaa gtcctgaaaa actgggacac
     1021 catcaaggaa gaaaccaagc accagaccac ccgcacccag acactggaag ctgtggcaaa
     1081 aaccctgccg gctctgatgc gtgcccagaa agtctgcaag cgtgccctca agggcaacaa
     1141 ccgctttctg tgcaatgaat ccgtcctcgc cagcgtgact gaatgcaagc acacgctgga
     1201 agaagccatg cacatggaag atgcggagca gatcacgcgg gcaatgggcg aactgctgtt
     1261 ctgctgtgcc ggcatggctc agacactggg gctttcggcg gaacaggtac tcaccgacac
     1321 caccaaccag ttcattgact gtttccgtgc catggaggaa acctgcaccg cggaaaagcg
     1381 ggaaatggaa accctctccc acggcgagtg gaacgccctc tggaagaaaa ccaaacaaaa
     1441 tttagaggag gaagcgaccc atgcggctgg ataaatatct gaagatctcc cgactcatca
     1501 aacggcgtac cgttgccaac gaagcatgcg atgccggaaa gattctcgta aacgacaagg
     1561 tcgcccgtgc atcgtatgat gtcaaggcgg gcgatgtcat cgagatccag atgggacaga
     1621 aggctctgaa agtccgtgta gaacaggtgg ctgaagtcgt ccgcaaggag gacgctgccg
     1681 gtctttacgc cgtggtttca gagtgagcag aagcagcatc acaaaaaaac aagatcggaa
     1741 agcggacggc atagactgtc cgcttttttg gtcactgttt tcataaacaa atcacaaact
     1801 ttcggaaaat ttgtttccgc ttttgacttt ttgcttttcc gcaaaaggca ccgctgtgac
     1861 agcagatggt gcaaaacgtt cattttatgc aaacaagtgt ctgtcttctg caatcatatt
     1921 ttttcttata catctataaa aaaacaaggc atttcgacgt tttttgtccc gcaggcatat
     1981 ttgggcaagt tgctattcat tcaacctaga aatttgcata aaatgcacga ggttttcaca
     2041 ggaatcttgg tagaatagta cattgacaat ttgcaacaga aaatgctata atttgagtat
     2101 agatatttcc gcacaaaacg gcgatgattt tttgtgcggc atcgtctgca atttgtgtat
     2161 aaaggggaac atcacagatt gacatggagt gtgcatgcac aaacacgtac acacaacaac
     2221 gaaatctatg agaaaaaatg gagggagtat ttatggaaaa tctaggttat cttctggaag
     2281 atgatgacat atcgcctgcg gtgaaacgcc gcagcatctg cacagctgga aaatgtttcg
     2341 aagacgtatc tgtttgtcaa gcgtctggca gacgtggtgc tgtcattcct ggcagtgatc
     2401 gtgttgagcg tgcccatgct gctgatcgca ctggcagtct ttttgcagga cttccacaac
     2461 ccgatcttca agcagctgcg ggtgaccaag gacggtcgct ttttcaccat gtacaagttc
     2521 cgcaccatgt acgtgaatgc cgaacaaaag ctggaggagc ttcgtgctca gaacgaggca
     2581 gacggtccgg tctttaagat gaaaaaggat ccccgtgtca caccgatcgg cagatttctg
     2641 cggaaaacca gtctggacga actgccgcag tttttcaatg tactgaacgg ttccatgagt
     2701 ttggtgggac cgcgtccgcc tcttcccaag gaagtggaaa agtacacacc gtaccagatg
     2761 caccggctgg acgcaaaggg cggtatcacc tgctactggc agtgttccgg cagaagcaac
     2821 atcatgtttg acgagtgggt ggaactggat ctgaagtaca ttcgggatcg ttctttcgcc
     2881 acagatgcaa agatcttgtt ccgtaccgta ggggctgttc tccggcggga cggtgcagaa
     2941 taaaggcaat accgcacaaa gagcatctgg cgactttgca cgccatctgc ttgcaaatt
//
LOCUS       DAWBGR010000231         2962 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_7655_length_2962_cov_4.566219, whole genome shotgun sequence.
ACCESSION   DAWBGR010000231 DAWBGR010000000
VERSION     DAWBGR010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7655_length_2962_cov_4.566219"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          615 a          847 c          743 g          757 t
ORIGIN      
        1 tggcaagccc caggcggaca gcgagtgcaa cgagcgaatt ccgccggacg gtttcagttc
       61 ggctgcacta gaagctgcac ttcatgtagg aacccctcca ccgcctaacg gcggttcccc
      121 tcgtactgag cggtcccctc tgccctgcgg gcatctcccc accccgtggg gagtcacccc
      181 tttcagggga ggcttattta cagcatgcaa acaagctgca cttcgcggct gacgcggggc
      241 gatgtaggca ttgcccacta cacattccaa gcaaaaacaa aaacaggctg ttgtacctct
      301 tgcaccggta caacagcctg ttctatttct acaaattcgc cgcgtgctta gtcatcactg
      361 tccagcttca gcacgctcat aaatgcttcc tgcggaactt ctaccgagcc cagctgccgc
      421 attctctttt taccctcttt ctgcttctcc agtagctttt tcttacgggt aatgtcgccg
      481 ccgtagcact tggcaagcac gtctttccgc atggccttga tggtttctct ggcaatgacc
      541 ttgccgccga tcgccgcctg taccggcacc tcaaacagct gccgcggaat gttctctttc
      601 agcttctcca cgatttttct tgcccgttcg tatgccttgt cggtgtgaat gataaacgac
      661 agagcatcga caatttcgcc gttcagcagt acgtccagtt tcaccagctt ggattccata
      721 tagtcgctga cttcataatc aaaagatgca tagccgcggg tgcgggattt cagcacatca
      781 aagaagtcgt acacaatttc attcagcggc agcacatagt gcagggacac gcgggtgtcg
      841 tccagatact gcatattgac aaacacgccg cggcggttct ggcagagttc catgacattg
      901 cccacaaagt ccgccggtgt caggatctcc gccttgacca tcggttcttc cgctttggcg
      961 atcactgccg gatccggata gttggtgggg ttgtcaatgt actgtacttc cccgctggtc
     1021 agggtgacac gatagataac agagggagca gtggtgatca gatccagatt atactcccgt
     1081 tccagacgct cctgaataat ctccatgtgc agcagtccca gaaagccgca gcggaatccg
     1141 aagcccagtg caatggaagt ttccggctca aaggaaaggg acgcgtcatt cagctgtaac
     1201 ttttccagtg cgtcccgcag gtcgccgtag cgtgcaccgt ctgccggata gataccggaa
     1261 aagaccatgg ggttgacctt gcggtaaccg ggcagcggtt ctgcacaggg gcggtctgca
     1321 tgggtgacgg tatcacctac gcgggtatcg ccgatgctct tgatcgaagc ggagaggtag
     1381 ccaacttcgc ctgcacgaag cacaccatcg ttgaccagcg tgttcgttgt catatagccg
     1441 gcttccacaa tggtgaattc cgcaccggtc gccatcagct taatggtatc cccgaccttg
     1501 acggtaccct ctttgacgcg gatatagatg acaacgccct tataggcatc ataatagctg
     1561 tcgaagatca gagcctgcag cggagcatcc ggatcgccct tgggtgccgg aatgtccgtc
     1621 acaatttttt ccatgacggc ttcgatgttg atgcctgcct ttgcggagat ccgcggggca
     1681 tcttctgcgg gaatcccgat cacattttcg atctcctcgc agacacggtc aggatctgca
     1741 gcaggcaggt cgatcttgtt gataaccggc actacttcca gatcgtgttc gattgccaga
     1801 taagtatttg ccagtgtctg ggcttcgatg ccctgtgaag catcgactac cagaatcgca
     1861 ccctcacagg cggcaaggct gcgggaaacc tcatagttga agtccacgtg accgggggtg
     1921 tcgatcaggt tgaacacata ggtttccccg tccttggcac gataattcag acggactgca
     1981 cgtgccttaa tggtgattcc ccgttcccgt tcaatatcca tgttgtccag gatctgttct
     2041 tccatgtccc gcttggcaac ggtataggtc tgttccagaa tccggtctgc cagtgtggac
     2101 ttgccgtggt caatatgagc aatgatgcaa aaattgcgga tatgttccat tctatgatct
     2161 tccttccatt tgagccgagc cggctctgtt ccgaaattac tccatggtat attataacgg
     2221 tttttcagga gattgtcaag gcacagcttt cccgccgccg caggagcatg cagttacgtg
     2281 ggatttttca gcgtgggcag gtggctgggg agatacccca gcacaatttt ttgcagttcc
     2341 tcgttccagc agaaatacag acgtgccagt ccctttgcct tgatgccgta cttgatgtgc
     2401 atgtccagca gatacttccg gtcgtccacc tgtgccagat aatcttcccg atgcacccgc
     2461 agggtttccg ccccgcagcg ggacacgtcc cagcaatatc gctgaccata cagttccagc
     2521 agttcctcgc tcaggttccc ctgccggaac tttccatagg cactcagata gaggataccg
     2581 tcacagatca ctgccagatc cagcttgctg ctgaatttcc gcagactgtt tctggcacgc
     2641 tgggtaaaga tcaggctgtc cgagaaatgc ttttccgccc actctggaat ggcttccttg
     2701 tctgtgggga atgtcgcggc aatgtctgcc ttttcccgat agaactgcac gatctccgta
     2761 gactgctgat aggaattttt tttcgaatcg tattcctgtt ccagaacccg atacgccgca
     2821 ttccgccgtt ccagttccgc ttctttttct gccagctgct tctgatagat tcgcagctgt
     2881 tccccattca tacgcatatc cgtgttctgc tgggtaaaat ctttcagctg gtttttcagc
     2941 tcgtcgtttt cctgctgtaa ga
//
LOCUS       DAWBGR010000232         2923 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_7800_length_2923_cov_2.746513, whole genome shotgun sequence.
ACCESSION   DAWBGR010000232 DAWBGR010000000
VERSION     DAWBGR010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7800_length_2923_cov_2.746513"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2657..2666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          905 a          537 c          600 g          871 t
ORIGIN      
        1 aaaaaagccg atacaagtgt gggggacagg cacgcctggc actattgtga cagtatcgct
       61 ttttacagca actgcggata ccatggtttg tgctgatggt tcatggaaaa ttgctttaca
      121 gccattatgt gccggagcag gatatacact tgtggcatca gatacagcgg aatccattgt
      181 gattcaaaat gtagctgttg gagaagtctg gcttgccggc ggtcaaagca acatggaact
      241 ggcactgaaa gacagtgaaa acggcagcaa gatttctgag gaatacagtg gaaaaaacat
      301 acggttttat caagttccga aatgttctat gctggatgaa aatcagaaag agcaggaaga
      361 aaacagcaca tggcagattg cggataaccg gaatgtgggc gaaatgtccg ctatagcgtt
      421 ttattttgca agccgtcttt cagaaacgct gcattgcatc gtaggtatca tagattgcta
      481 ttggggcgga agttccatta cctgttggat gagcagagaa cagcttgcaa aactggaaag
      541 cggaaagaat tatttggaaa actatgctgc tcttgtggga gaaaaatcct ctcagcagta
      601 tgcaaaagaa atggaagaat acaactgtgc atatcagcac tggttaaaaa gcacagaaga
      661 agtaaaacag aaagatcctc aggcatcgtg gaaagaaatc cataaaattt gcggagaatg
      721 tccgtggccg cagccagttg ggtggcagtc gccgtttcgt cccaatggac ttcatgaaac
      781 aatgattcgg cgtatagcac cttattctct gcgtggattt ttatactacc agggagaaga
      841 agacgtgcag cggtacgcag attattcgga tatgatgctg atgcttgtaa aacaatggcg
      901 aatggattgg aatgactgga atctgccgtt tttgtttgtt caactgccga tgtatcttgc
      961 agacggtgaa aaagatgaca gttattgggc attgcagaga gaacagcaat acattgcctc
     1021 catgatgatt caaaatgccg gcatgatctc tctggcagac tgcggtgaat atgacaatat
     1081 tcatccattg gataaaaaaa caccgggaga acgtttgttt cagcttgcga aaaatctggt
     1141 atacgacaag cagggaacga tcacagcaac agcggcacag ctgttttcag acaatgggaa
     1201 gctatatgtg acatttgatc atctgcatga aaaattggtt atgcagacag cgaatggctg
     1261 cccttttgaa atttctggag aagacaatct attttatcct gcaaaggcag acgtaattca
     1321 cggaaatatg ctctgcatat caaatcccgc agttaagttt ccatctgccg tcaaatatgc
     1381 ctggtacaat tttggtgagg cagtcttgtt tacagaaagc ggaaatccag tttctccatt
     1441 tttcaaatgc gtataaaaac atgacgggaa gtatgattca tgcaaaacat agtcaatccc
     1501 aatgcaacag aaaaagcaaa agcacttctg aattttcttt cagaaaccgc aggaaaagcg
     1561 atcatcaccg gacagcacac ccagacaaat ccgatggagg aaattgacta tatcaaaagc
     1621 aaaacgggaa aagagccttt gctccgcggg tttgaaatgc tggcatattc accgaacatc
     1681 aatgataacg atgtgtcaga agcctgcctt actgaggttt atgaaaatcg aaataccatg
     1741 gaaactgcac tgaaatgggc aaaagcgacc ggcggtattg tgacattgaa tattatggtc
     1801 gcattggcga atctgtatct ggctgccaga aaatctctga cggtctgatt cagtgcatct
     1861 ttgcgatgga aaatggaaca tttctctaac cactcagcat tttgctgggt ggttttgttt
     1921 gtaggtggag attatgcggt gttgccctaa atcgtaatct agcagcggct gcctttcaaa
     1981 cgcatagaga tttcgaggtc ttggggtatg acttttgtga ttagttgctt ttcaacttct
     2041 ttcatggact tcaattcctc tcttagagtt ttttattctt tagtgcattg gggttatgaa
     2101 cagttcacaa tgcctactgt acagtttcac atctcattaa atcacaattc ctcaacgaat
     2161 tttgtatatt ttccaagaaa aagttcctca atcttaatcc attttgtatg aaaacatccg
     2221 gattcttgac atttttcttt ttgatatgca cctttcggca tctgaactgg caattcgcca
     2281 agatagaaga acgtgtccga catatttcgc ttagaagtca taatcaatcc caattcataa
     2341 tcaaaacaaa aatcgccgat tggtctgcca tgttcatctt ctttttcata tcgttttgca
     2401 attggaataa atggatatgt tgacacgtca tggaacaatt atttaatcca cttttttttg
     2461 taaggattat atattaactt ccaataactt ttaggaaatt ttttgagatt attggcaaca
     2521 atttcaggat acatatgcaa tgcatcagct aaatcaaatg cctgatcata ttttttttgt
     2581 ataagaagat tctctagctc gttcatattt gtgattaaaa attctatttt ttctttgtcc
     2641 tttaataacc ctgattnnnn nnnnnngcat agaacactcc atttatcatc acatatatta
     2701 agactaatca ttaattttcg aagtcgatta tcatcaattt cttcaacgcc atttttatat
     2761 aaatcagtaa ctttttttaa tatatatata ttattcttgt cagttatttc atttctaata
     2821 actgttatta aataataaaa atcataaagt tcatgagtag taattatcat attaatcatt
     2881 ctccaagaaa tcattttata ataacatcaa tggttctttt atg
//
LOCUS       DAWBGR010000233         2922 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_7804_length_2922_cov_4.808162, whole genome shotgun sequence.
ACCESSION   DAWBGR010000233 DAWBGR010000000
VERSION     DAWBGR010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7804_length_2922_cov_4.808162"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          606 a          767 c          736 g          813 t
ORIGIN      
        1 ccccctcccc tttcagggga ggcttttttt ctaggctccc ctgaaagggg agctggcaag
       61 ccgcaggctt gactgagggg ttatctcgca gcctttttta ctgttccgtc aacgcagacc
      121 actctgtgat ctcgccggtg tagatctgct tgatctgatc cttggtaatc gcgtcgagag
      181 aattgtcctt gttgacgatg actgcaatgc cgtccattgc cagaacagtg ctctttacgc
      241 cggtttcagt gtccttcagt tcgcgggaag ccatgccgat gtcgcatacg ccctcctgtg
      301 tggaagtcac gccggtggtg gagtcgctct gctgtacgtc tacggtcacg tccggattca
      361 cgtccgcgta agcctctttc agcttagtca tgaccggtgt gacagaagaa gaaccggcaa
      421 ccaccacgct gccctttgca ccgttggagg tatagctttc tgtgccctgt gccacatagc
      481 cctctttgct gatgacatct gcggcatcgg tgctcatgat gtagttgaag aaatcctgtg
      541 ccgcatcgct cagttcctcg ccgtccttgg tgacgatgtt gaacggacgg gaaatggaat
      601 agctgccgtc ggccacggtg tccacgctgg gtgctacgcc gtctacttcc agagccttga
      661 cggaatcgtc cagagagccg acagaaacat agccgataga agccgcattg ccggcaacgg
      721 tcgtcagcat aactgcggtg ctgttcgtga cctctgcact ggaaactgtg ttgtccttgt
      781 cgtcctccat gatgccgcac agttcggtga atgctccgcg ggtgccggaa ccgtcctcac
      841 gggtaatcac ggagattgct ccggaagcac cgccgccgca gcctgtcaga ctgcttgcca
      901 tcatgcccaa tgctgcaact gctgccgcga tctttgtcat ttttttcata acgcataact
      961 ccatttccgg acacgcttcg ctgtgtccct tgccgattct gcccagaatc gtgtttcgtt
     1021 ttttattctt gttccaatga acaatgttat tataacaagc ctatgtaaag tctgttatcg
     1081 cggcaatgtg aaatctaggt taaatgttaa gttttgcggc atgtcctttg ttaactccga
     1141 aaaaaacacg gaatttcggt atattttcag ccggcatgca ctgagaggac agattccggc
     1201 agtgtgttcc ttatgtcagg ttatagacaa atgacgcata agcctctgca ttttcacacc
     1261 gcgggggctg gggtttgtcc ggacaggacg gcttttcgtg gcgttcccat gtccgcagag
     1321 ccgctttcca gtcccgcatc gggtgctttc cgatgttcca gccgttggct tcgtagtagt
     1381 cgtaaaactg ttctgccgat accgtgctgc cccgatgcat gcagtagttc cgtatctcct
     1441 ccagtgtcgg cttggaaaaa gcagtccttt ttttccggct gccggttgtg gtggttgttt
     1501 ctggaactgc cgcggcagcg gtctggctct ccggaaacaa ctctttctct gcatctgact
     1561 ctgtttcttt ctctatctct aactctttct ctatctcttt ctctacgtca caatgtgcag
     1621 ccggtgtgtc acaatgtgca gcttcggtgt tacaagcttc tgccggtgcc tgaaccggtt
     1681 cctgcggctt ggcatctgcc ggtttctgcc gtgcttttcg catgcgttcc gcagaagcac
     1741 tttccgaacc cgtcaccgca cctacctccg gaagatagta gaccgtttcc gacttctgtt
     1801 ccagccggtg cacacgcatc agaaactcca gcgtgtccct gaccagttct tcgtcctcgt
     1861 ccagtgtcag tgccagttcc gtgtgaaaat ccggctccag tccgtcgaag tacaactcgc
     1921 cgctgctgga aaggcttagc aacagcattt tcagatagat catcacgcag gtatccccgt
     1981 tgggcattcg ccgcagtttt ttcactgcct tgtcctgaaa gaaatgttcc tgtaatttca
     2041 gccaataaaa ccgtttgctt tgcatgtgca gttcctcctg ttcccgcccg tccggagcac
     2101 gcttcgggct ggcatttcgc aatgtaaaaa agcccctcca tatataggct ctcagagggg
     2161 aaaagttgca cgccggcgtg caactttgaa cgagaattta cacagtttct gatgaaagtt
     2221 cggaatattg gaagtttgct ggaagtttcg ggcaggttct gttgggagag gcagtgctgc
     2281 ccgggcaaac agatcgaaca tgacatgcta tactattata atagtataga cgtgcaatgg
     2341 ctcgactgca cggcagcaaa aaagccgcac cacccaccgg aacgctccgg cagtgatgcg
     2401 gctgtttgac attttttgca aagtgtggta tactgaattt tgagggcata cctgaaacgg
     2461 taggcggtct ttccacgctc ccatgatttt atcaaaatcg atgggaggtg atgtgcatgg
     2521 agaactttgt aacatgggag cagctgcgtt ttttcgcatg gctcatactt acattgattc
     2581 agatagcgat tctgcgttcc aatcatcatg atgataaacg gaaataaccg ccctacagct
     2641 gcaactcaag gcggttattt taagtaactt attgggagca gaccgtctac tggtatgctc
     2701 tttctatctt tattatacgt cagaacgccg catttgtcaa gggctttttt gccgggaaat
     2761 gcggctgatt ttatactgaa aactatcgtt tgacattttt tgcaaagtgt ggtatactaa
     2821 attttgaggg catacctgaa acggtaggcg gtcgattcct gctcccggaa gggagtgagt
     2881 gcaaatgaac ctggacattc tcgtcaagtg cattagcatg gt
//
LOCUS       DAWBGR010000234         2903 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_7874_length_2903_cov_4.474719, whole genome shotgun sequence.
ACCESSION   DAWBGR010000234 DAWBGR010000000
VERSION     DAWBGR010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7874_length_2903_cov_4.474719"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          743 a          705 c          823 g          632 t
ORIGIN      
        1 acagttgaaa ttggggcgta aggtcagcgg cgacccgccg cgggcgtagg gtcagcagcg
       61 actcgccgct cgccgcactt ctttttcgct ttttgcatac gttaagaaca ggagtttgtg
      121 tatttgcaca actttctgac atttggtgct gtgatgatgc actgcctgcc tttgacgtgc
      181 aattcgccgg cagacagtgc tttccacagg ttttccacag ggaaagaagt tgataagttg
      241 aaaatctgtt gaaaactaag tcgatttccc ctgtggaaaa ccctgatttt cagcaagcgg
      301 aggaaacgtg tatgaaatcc gacaaagtca ttcttgtcgc aggcggtgat ctgcggcagc
      361 agtatgcggc acagcggctc cggcagaaaa ccggtgcggc ggtctggacg gtgggactgc
      421 cggcacagcc gaacctgcac ttcgccgaaa caccggcaga ggttccggca tacgacgtgc
      481 tggtgctgcc tgtccttgcg ggcagcagcg gggaaaccgt gccggctcca ttcggaacat
      541 ctcccctgtc gctgcccgaa ctggcggcac agggaaaacc ggcggcactg gtcgccggcg
      601 gcggttgctc cggcgtggca cattggttcg aggctgccgg catgactgtg accgactacc
      661 tccggcggga ggaactgtgc ctcgccaatg ccgtccccac tgcggagggg gcgatccgga
      721 tcgccatgga agaaactgcc cgcaccctgc acggggcatc ggcactggtg gtgggctatg
      781 gcaggatcgg catggcactg gctccgcggc tgcgggcgtt gggcatgcag acggagatct
      841 gtgcgcgtcg ttgtgaaacc cgtgcactgg cacagatgca gggcttttcc gccgtgccgg
      901 tgtccgctct cgcccaaagg gcagcagcgg cggacgtgat cttcaacacg gtgccggtgc
      961 tgctgctgtc tgaaacggtg ttgaaagccc tgccgccgga aacgctggtg atcgatctcg
     1021 cttcccgccc cggcggaacc gacttcgatg cggcaaaacg tctgggcatc cgcgtggtgt
     1081 gggcattggc cctgccgccg gaagtgcggc gttgccacat tccggaaaag gcggtttctg
     1141 cggaattcca tagaaaaaat gttgaaaagt cggtggaaag tgtgttgaat tcagaaaaac
     1201 ggcttcgaaa ttgtgaaaaa gcgacaaaaa cggggataat atccagcaaa gaattttaaa
     1261 aaaaatcggg ctttgggctt gacacgggca caataatatg ttaaaatacg tatgtaaatt
     1321 gtttacgagg gcttttttat aggaccttgt agacataaga acaattgccg cagcgtgcgt
     1381 ttccggcaga tgaagcggga ttaccggaag cgaatgcgtc tgatgcgggc ggtggaacac
     1441 cacctgtgcg gacggcaatc aatttaatta aaaagagagg taataagaaa tgaagaagac
     1501 atttaacaga cttcttgctg caactgtcgc aattccggtt gcactgggtc aggttcttgc
     1561 aatttctgca aacgctgcgg aagctcctgc agcactgaag gttacagctg acaagctgct
     1621 gaaggtggaa ccggctactg gtttcccgga aactgttagt gcagatgcag acactattac
     1681 ttacactgta gagtccgatt ggaacactac tctggcaaag cagctgaaca cagaaacttc
     1741 caacaagact gtgactgtgg acgcaaagaa gtttgtcgca ggcatcaact ccgcaaacta
     1801 ctatgtagaa ctcctgaaga aggcagtaga cgcatctgaa aatccgaccg ctacggtaaa
     1861 ggatggcgtg gttaccattt ccggtactgc tgatttcagt gctgcaacag acaagctggc
     1921 tgaaaagctg gacactctgg acggctatga aaatttcact ctggatactt ccattttgac
     1981 tggtgttgct tacactgcaa ccatcaagac agatttcgca aacagcaaga gcgttgacgc
     2041 tgatctgagc ttcaccgcaa acggcaagac ttatggcgta agcacagcaa ctgagtatgc
     2101 aaacgatgta tacaccaatc tggctggtca ggtaactgct gctgtagacc agaaggtcaa
     2161 ggaactggct gctgaataca acatgaccga agatgaagtt cgtgcaaatg ctgacttcga
     2221 catcgctggt gatgttgctg ctctgaaggc agaaactgac aagctgtctg ctgacattgc
     2281 aaaggcacag aagaagtacg attctttcaa gaatctgaca aaggctgcaa agagctatga
     2341 ttccgcagac gctgctctgg ctgctgctgt aaattatctg tcaaagaacg ttgcagctgc
     2401 tgcaaatcag ccgaagacag ttgatggcat ggttgcaaag tatggtgcaa acttcgataa
     2461 cggcgttgca tctgtaaaca gcagcctgaa ggacgctggc gtaaacgtag agatcgctgt
     2521 ttcctctgct gatgttgctg ctctgctgaa gagtgcaaca aaggtaacca ttggtgcagc
     2581 agctggcact tacactgctg aactggaaat cactgatgct gaaaaggctg aagtagaaaa
     2641 gtacgtagaa gaacaggttg cagaaaagct gccggaaaag accgttgttt ctgttgacac
     2701 cgtaaagacc gtaacggttt ccggtgcagc tgacggcgtt gctgctttcg atgtaactcg
     2761 tgacgtaacc gttgttctga aggacaagga tactacaact actacaacaa ctaccactgg
     2821 cgacagcggc acaactacta ccgatgcaag cggtgcaact accgatgtca gcggtgcaac
     2881 taccgatgta agcggtgcaa cca
//
LOCUS       DAWBGR010000235         2901 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_7879_length_2901_cov_2.747716, whole genome shotgun sequence.
ACCESSION   DAWBGR010000235 DAWBGR010000000
VERSION     DAWBGR010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7879_length_2901_cov_2.747716"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          923 a          664 c          731 g          583 t
ORIGIN      
        1 atgaagaagc tgccgacgat cgcatgatcg atctggcggc tgatgttctc gttgcgattc
       61 tgatggagga gcgtgcatca tgagatactg ggtcgtacga ccgccgccgg agtgcggttg
      121 gagtggcaag aaaaaacaga agggaaaggg attttgcaat gaatacatca aaaaagacgc
      181 agcttgcact cgtctatatt cgctatagtt cacatgccca ggacaacggc aatagtccgg
      241 cggcacagat gacgtgtgcg gagaattaca cagaagcaca tcagatgacc attgaacaga
      301 tctatcacga ttacgccaag actggcagaa acaccaatcg ggaaggctac cagcaaatga
      361 agaatgatat cctgtccgga aaagtacagg caaagatcgt tgtggtaaga gcactggacc
      421 ggctccacag aaacaccggg aatcagatcg aagacctgaa atggatggaa tcacatggga
      481 tccggctgat cgctgtgaat gacagcattg atacagcatc tgaggactac agcaagtttg
      541 ccacgatcgt aaaggcggca gcggcagagg aaacatcaga caacatttct aaaaatacca
      601 gagcggcact gctggaaagt gccagacagt gccgtcatct gggcggcgtt ccacccatcg
      661 gctatacagt caatgaggct ggactctatg agatcgatcc tctgacagca ccgatcgttc
      721 gtgacatcta taccctttac agcagcggca tgggctacag ctacatcaaa aaacatctga
      781 gagaaaaggg atacaagacc accggcggca acgacttttc agacaccgcc atccacacca
      841 tcctgacgaa ccagaaatac aagggcactt acacctacga ccgcacagct gccaaggaca
      901 gcgacggaca ccgaaactcc cacaagatca agccggatcc catagagatc ccggacggga
      961 tgccggcgat catcgagccg gcacgcttcg acaaggtcca ggagaagctg gcacagaacc
     1021 gccggcggac agccagccga accggcaaga actactacgc cctcaacggt ttcacacgct
     1081 gtccgaagtg cggcaagccg ttctccggca acgtcaacca cagcaacggg cacaagtacc
     1141 tgcagtaccg aagaagctgt gactgcggca tcccagcgtg cgggcagacc agctggcacc
     1201 gtttgtgttt cacgcagtga agaactgtct ctttcagccg gccaaccagc caaagatcat
     1261 cgaggcagtc aaccagaagc tttctctccg aaaactgatg cagagcggag agaccaacac
     1321 catacaaaat cggatcaacg gactggaaaa ggcaaacgcc aatctgatgg catacctgga
     1381 acagggacgt gctaccgaaa ccatcctgcg ttccgttgag aaaaacgaac aggaactcca
     1441 gcagctgaaa cagcagctgg atgctttgga caatccgatc tctgcggtga atgatgcaac
     1501 ataccaggag atcgttcaaa agtttacaaa ctacatgtgt actgaaagat cgccggaggc
     1561 agctgcactg aaagaggctg tgctacggga cattcagatc ggtaaagaaa ccatcaccgt
     1621 gaactttcag cacggtgttt tcatagatga agaaacaaaa gcatacttca acgacaacag
     1681 aaaggaattg gcatcatgac atatcaattt gaaaatccca ttgcgtgtac cgaagcactg
     1741 gtaactgcaa ttgagaaatt gaacgacttt gaacaatata cgctgacgat tctgggaatt
     1801 gaccagaatg gctgttacac caacaaggga accgtgacga tcaacgaaga agaactgcag
     1861 ccgcttctgc aggagaaaaa gctttccttt ccaaaaggca tcatcggaca aagagtcaag
     1921 atcatctctc tcgagaagct gcggcaggaa caggcaaaag cagcactttt ccagaagatc
     1981 gcagaagacg gcgaagaaat caacgtggaa ttttgaaagg ggctattttt atgaagcaga
     2041 ttttcgtagt tacttataac tataccaaca agaagttagc acagatggca attgcttgtg
     2101 tcatccgatg cagaagtacc catggaaagc agctgcgtgt acgggacatc attgaaatga
     2161 accgtcccgg actatatcaa cagctatctc gtggagataa gtgcagagtg ggcagagccg
     2221 tcagcaagct gtacaatcag ggaatgctgc cgcatctgac caaaggaaag aagaagggtt
     2281 caaccaatac gtacttctgc taagtagcag aaagaacatg tcacaatgag aaagctccgg
     2341 atcgtcctaa gtggcgggaa aatccggagc tttttgtgta tgaaaaagtt gctttttccc
     2401 ctctgctgtg gtataataaa gggaaagaga ttgaaaacac agcaaggagg gaaatgcaat
     2461 gggtgtttac ttgaatccgg gaaatgttga ttttcagaag agcttaaatt ctgagattta
     2521 catcgataag acagaactga ttgcgtatac gaataagaaa ttaaatacag aacaggggta
     2581 catctgtgtc agccgtccac gaagattcgg aaagtctatg gcggcgaata tgctgacggc
     2641 ttactacagt cgtggctgtg attccagaga aatgtttcaa ggactgaaaa ttgcaaagca
     2701 tacgggattt gaagagcatc tgaatcaata caatgtggtt cacttgaata tgcaaaactt
     2761 tctgagtaaa acacaaacca ttgaacagat gatttcgctg gttacaaaag cagtagggcg
     2821 tgatctgctg cgggcatatc cggatgtgga ttatttggat aagactattt taacatttat
     2881 gttagatgac atttatgagg a
//
LOCUS       DAWBGR010000236         2893 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_7907_length_2893_cov_3.523961, whole genome shotgun sequence.
ACCESSION   DAWBGR010000236 DAWBGR010000000
VERSION     DAWBGR010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7907_length_2893_cov_3.523961"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          731 a          821 c          752 g          589 t
ORIGIN      
        1 atctgccgaa aaatctgacg acgcatctta cgcacaagct ttatgcagtg tttcccttaa
       61 caggtaaagc aacagagtag ccggcagttt ttcactgcca aaacgagaaa aggacggcga
      121 tcatattgta taatccgcaa tcccttcggg cggaagaatt tatcaaccat gcagagatcc
      181 tggacacgct ggaatacgcc agagcacaca aaaacgacac ttccctgctg tcagaaattc
      241 tggcaaaggc ggcacggcga aagggactga atcatcggga ggcggcggtg ctgctgctct
      301 gtgaagatga tgctgtgaca gagcagatga atcgtctggc aaggcagatc aagctggatt
      361 tctacggcag ccgtattgtc atttttgccc cgctgtatct gtccaactac tgcgtcaacg
      421 gatgtaccta ctgcccctat cacgccaaga acaagcacat ctcccgcaaa aagctgtcgc
      481 aggaggacat tgttcgggag gtcacggcgt tgcaggacat gggacacaag cggcttgcca
      541 ttgaagccgg cgaagacccg ctgcacaacc cgatctctta cattctggaa tgcatcgaca
      601 ccatttatca cattcaccac aaaaacggag caatccggcg ggtgaatgtc aacatcgccg
      661 ccaccacgga agaagaatac cacatgctga aggaagccgg catcggaacg tatatcctct
      721 ttcaggaaac ctatcacaag gaaagctatg aaaagcttca ccccaccggt ccgaagcaca
      781 attacgacta ccacaccgaa gccatggatc gtgctatggc tggcggcatt gatgatgtgg
      841 gactgggcgt gttgttcggg ctggaaaatt atccctacga gttggtaggg cttctcatgc
      901 acgcagagca tctggaagct gtccacggcg ttggtcctca caccatcagc attccccgca
      961 tcaaaaaggc agaggacatc aaccccgacg atttcgacaa cggcatcagc gacgatattt
     1021 tcgccaaaat ctgtgccctc attcgcattt ctgtccccta caccggcatg atcatttcca
     1081 cacgggaaag tcaggcagta cgggaacggc tgcttccgct gggcatttcc cagatcagcg
     1141 gcggctcccg taccagcgtc ggcggctacg atataccgga aacacctgat gacaattccg
     1201 cacagtttga tgtcagcgac cggcgttcgc tggacgaagt ggtgcggtgg ctcatggaaa
     1261 tggactatat ccccagtttc tgtaccgcct gttatcgtgc cggacgcacc ggcgaccggt
     1321 tcatggctct ctgcaaaagc ggacagatcc agaactgctg ccaccccaac gctctcatta
     1381 ccctacagga atttctgacg gactatgctt cgccggaaac cagagcactg gggcaggaag
     1441 tgctgaagcg gcagcttctg gaaattccca acgaaaacgt ccgtgccaaa accgaaacct
     1501 atctgcgtga aatcctgcaa ggggcaagag atttccggtt ttaaggagaa acgcttatgg
     1561 aacagaaccg caaccagaca ccccgtgcca atcgggtaca cattggcttt ttcgggcggc
     1621 ggaatgccgg taagtccagc ctgatcaaca ccgttgccgg acagacgatc tctgtggttt
     1681 cccctgtctg cggcaccacc acagacaccg tggaaaaagc aatggaactg ctgccgatcg
     1741 gagcagttgt gctgatcgac acccccggtc tggacgacac cggcacactg ggagaactgc
     1801 gggtacaaca ggcaaagcgg gcattttctc acacagatct ggctgtgctg gtaatcgatg
     1861 cctccaccgg catgactgaa gcagaccata ccattttgca gcagctggaa caacagcaga
     1921 ttccggtgtt gcaggtttgg aacaaatgcg acatcgcaga cagcgttccg gaaaccggca
     1981 ctgctgtcag tgccagaacc ggacagggta tccagatgct caaggaacag ctgggaaaac
     2041 tctaccagca gcatgccgaa aagcgaccgc cgtccagtct gttcggcagc ctgctccgac
     2101 cggaggacgt ggtgcttctg gtcactccca ttgacgaatc ggctccgaaa gggcgtatga
     2161 ttttgccgca ggtacaggca attcgggaga ttctggatca gactgccgtc tgtctggtga
     2221 cacagccgga acagctggct cacacccttt ccctgctgaa acagccgccg cggctggtgg
     2281 tcactgacag tcaggcgttc ggacaggtca aatcactggt gccggaggag atcttgctga
     2341 catcgttttc cattttgttc gcccgctata agggcgttct cgctccggca atgcaggcgg
     2401 cacagactct ggaaaccctt ccggaaaaca gccggatcct catttctgag ggctgcaccc
     2461 accaccgcca gtgcaacgac atcggcacag taaaactgcc ccgctggatc actgcacaca
     2521 ccggcaagca attttcgttt tcgttttcct ccggcaacag ctttccggaa gacctcacgc
     2581 cctaccgcct tgtggtgcac tgcggcggct gtatgctgaa ccagcgggaa atggaaaccc
     2641 gtgccgcgag agccgctgca cagggcattc cctacaccaa ttacggcgta ctgatcgcct
     2701 acctcaacgg catcttagag cgaagcctga cgtttttgaa agccctggaa aactcggaat
     2761 aaaggaaaca cggaataaaa atccctgctc cggatcatac cggaacaggg attcttttct
     2821 gtctgtagga tacaggattt tttagggcaa taccgcacaa agattgtgcg acagatgcgt
     2881 cgtcaaattt ttc
//
LOCUS       DAWBGR010000237         2874 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_7964_length_2874_cov_5.584604, whole genome shotgun sequence.
ACCESSION   DAWBGR010000237 DAWBGR010000000
VERSION     DAWBGR010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7964_length_2874_cov_5.584604"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          691 a          742 c          830 g          611 t
ORIGIN      
        1 tgcaagcaga tggcgtgcaa agtcgccaga cgctctttgt gcggtattgc ctaaaaaaat
       61 tcgggaagca ctgcataaag cctgtgcggt gtttccttgg gaaaggtcga atcatatgct
      121 gtacatatta ctatttctct gcttgataca actggctgcg atcatctggc tggcattaca
      181 ggtacagaag ctgcggaatc cccaggacga caacaccctg cgaaccctgc ggcaggagat
      241 cgctgccgac acccagaacc agatgcagat gctggggaat ctgctgtcgg aaaatcagcg
      301 gcagtccggc gactatcagg cagaacgact ggaacaaatg gaacggaaca tggacgcacg
      361 gctgcggcag tttgaaagtc gtgtgggaaa tctggaacgc tccaatgcac agggcatgga
      421 gcagattcgc tctgccgttg cagcaaatct gcaccgcatg caggcacagc agcaggacag
      481 tctggcagag atccagacca ccgtcaacga gaaactggca cagtccctga accagcagat
      541 cggacagtcc tttcagctgg tcagcagccg gctggagcag gtctatcagg ggcttggcga
      601 aatgcagacg ctggcggtgg gcgtaggcga cctgaaaaag gtgctgtcca acgtcaagac
      661 ccgcggcatt ctgggagaag tacagctggg ggcaattttg caggaaattt tgtcgccgga
      721 gcagtacgcc accgagatcc ccactctccc caacagccgg aaccacgtgg aatttgccgt
      781 gcgtatgccc gcagacgggg aacagccggt atggctgccc attgactcca agtttccggg
      841 ggatacttat gcggcgttgc aggacgcacg ggaacagggc gatcctcagc aggtggaggc
      901 ggcacggcag aaactgctga ctgttgtccg cggctgtgcc aaggacattc gggacaagta
      961 tgtccagccg ccgtatacca ccaacttcgg aatcctgttc ctgccctttg aggggctgta
     1021 tgccgaggta gtcaacagcg gtctggtgga acagttacag cgggaataca gtatcagcgt
     1081 tgccggtccg tctactatgg cggcgattct gaacgccctg caaatgggct tccgcaccct
     1141 tgccattcag aagcacagcc acgaagcatg ggtggtgctg ggagcagtcc gcggggaatt
     1201 cgaaaaattt gccggcacga tcgaaaaggc acagaaaagc ctgcagggag ccgaacagca
     1261 gctggaactt ctggcaggca cccgcacccg tgccattgtc cgtcagctgc gggaagtgga
     1321 acgcttacag ccggaggaac tgcgaaagta atcaaggcaa taccgcacaa agtcgccaga
     1381 cgctctttgt gcggtgttgc ctcaagacag acaaatgccg cccattccca acaagggaac
     1441 aggcggcatt tttacgtgaa gacaggttct tattcttttg aggtgtcctt ttcggcttcc
     1501 gttctctttc tggaaacgcg gctgcggagg gactgcacag attttcggat atgccactgc
     1561 cgcggtttgg gcgtggactg attctccggt gaaccggtgc tgccggtggt cttttcccga
     1621 atcacggtgt aaatgataga catgatcgga acgccgacga tcatgcccag tacgttgaac
     1681 aagccgccgc ccacagtgat ggcgaacagc acccagattg ccggcagtcc gacggaagtg
     1741 ccaaccacac gggggtagat caggttgctg tcgatctgct ggacaatgat aacgataatg
     1801 acaaatgcaa tggcatgact gggcttgacc atcagcagga tcaacgcaca gggaatcgta
     1861 cccatccacg gtcccacata ggggatcatg ttggtgatgc cgatgataac cgcgatcatc
     1921 atggggtatt ccagtccgcc gatactcatg acacagaaaa tcagcacgcc cagaatgaac
     1981 gcatcagaca gctgtccgct gacgaaatta gagaacgtgg aaaaagtcag tctgccgtag
     2041 tgggcaacct gttcgtagtg ctttttcggc aggaaatgcc ggaaaatcag ctttgcctgt
     2101 ctgcggagtt tgttccgtcc ggcgaggaca tatacggaga agatcagccc gatgaaaaag
     2161 tcaatgacac cgccgatcag cccgcttgcc catgtactca gtgtgcccac tgcgtccgga
     2221 atgtaggtgg tcaggctcat taacttggtg cggagcattg cgggattgat gtcgttgtcc
     2281 tgtaacagtt tgagaatctg ttcgccgcgg tcgctgttca ggaatttcat gatattgttg
     2341 tagtatacca tgatgttgtc cccgaagtcg cggatactgg tgatgaactg cggcacaatg
     2401 atccagatga caatgctgag cagtgccagc atgaggaagt atgcacagaa caccgccatt
     2461 gcaccggcag ggaaccgcgg gaatttttgc tgtaccttgc ggaatagctt gtcgatctga
     2521 caggtgggac ggtataggac aaatgcgatg accagcccga tggtgatcgg ccgcaggaca
     2581 tcgaacacat aggcgagcag tccggtgaag atttcccgct tgatgaacag gaacgccacc
     2641 aacagcagca ctacgccgct gaagatgatc gttgccgccc actgtttgtt gacaaagtcc
     2701 cacttgcgga cttttttttc gtcttccttt tgagaatccg tcatggtttc ggtttcgttt
     2761 ggttctgttg tcataatgcc ctcctttgga atggcggcgg gtgatttcag attcgcttgt
     2821 ttaggaaaca ctgcataaag cccgcctgac ggctttgcac gcaatctgct tgca
//
LOCUS       DAWBGR010000238         2862 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8014_length_2862_cov_2.551478, whole genome shotgun sequence.
ACCESSION   DAWBGR010000238 DAWBGR010000000
VERSION     DAWBGR010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8014_length_2862_cov_2.551478"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          831 a          650 c          646 g          735 t
ORIGIN      
        1 aaaacatctg cacatataca aggaagtgca gtcagcttaa ttttattcat tataccacct
       61 cacaaattcc gatttatagg gctgtcacgc cctacagcat ataacccaat tatatcacat
      121 tccactctca aaagtcaatg aaaggagctg aaactttggt tttctctctc atcctcgaaa
      181 acgccgccgg cgaacaaatc gacctaacca ccacagccaa ccgctatatg gtctccgaaa
      241 tcgacggtct gtatccccct gccggcacag tcagcacctc cacctatgcc ggcatgaacg
      301 gcagctatct gaacaacgca ttcatcgaaa agcggaacat cgtcattacc tttcagatgc
      361 gtggagtgaa catcgaaaaa cgccgtcacg ccttgtaccg tgtggtgaaa ccctcccgct
      421 atctcaagct ctattacaaa acggcaggca ttgacgtgtg caccgaaggc tatgtggaaa
      481 cctgccctgt ggaacacttc agtgataagg tcagcggtca gatttcaatg atttgtcccg
      541 acccgtactt ttacagcacc tttgcaattc acgcctacta cagccagatt gcaggggcat
      601 ttgtatttcc ctttccgcaa agtgacgagc cgtttcccct tggaatttac agcaccacgg
      661 ataatatccc catacaaaat gacggtgatg aaacaggctt taccattcgc atagaagcat
      721 gggcaaatga aacagtgccg gaaatcgccg cagtaacacc aacgatatac aatgccgaca
      781 ccggcgaata tcttcaaatc aaaggcgaaa ttctcaaagg agatgtcatt acaatcacca
      841 ccaaaacagg cagcaaaacg gtgacgctca cccgaaacgg cgtggatttc aacatcatca
      901 atcgactggt ggcaggttcc acatggctga cgctccgtga gggacaaaac acgtttcatg
      961 taaccgccgt tcgcaatgtg aaaaatatcc ttgtgacctt gatgcacaca aatgcgtatt
     1021 taggagtgta aaagatgcag attgaaattt attgttttac ggcaaaagaa cgcagtatct
     1081 cagcagagct tatggcaatc tgcgacagtt tttcttcgct gctctgggac gtggaatatt
     1141 acaaatgcgg ctgctttgaa gtgtacattg ccgccaatgc caaaaatgtg gagattttcc
     1201 ggacaggaag aatcatcggg cgtaatgatg acaaggaaca cttcggcatc atcgagtcgg
     1261 tacaaattga taccgatgca gaaaatggtg attatctgac cgtttcaggc agattcctga
     1321 catcactgct ttcccgcaga atcatttacc cgtcattttc cgcaggcaat acctttgaaa
     1381 atattgtgcg gagtgtgctt tccgacaatg ccatatccgc tggcacccgc accatttccg
     1441 gactttctat gggaacagtc agcggcgaat gctggcaaag cagaacaaga ctgcaagtca
     1501 gctatgataa tctgatggaa tggctgtata ccatttgcga aaccattggc ggttcggtaa
     1561 atatccgcat ggaaggcaat gcactgaaat gtgatcttct gcaaggcaca gaccgcagta
     1621 ttttacagaa cgacaatccc catatcgtcc attgctgcat tttccacgca gtatcagtac
     1681 aacaaagact attttgtcgg cgattatgtc actgtgaagc atgtgcgttt cggactcata
     1741 cagccgaaaa ttcagcttgt gggcatgatt gaaagctttg atcagaacgg cagaagtctg
     1801 acaccaacat tcaaaatata ggaggtactt atggcatttt catatggatt tttcaatgca
     1861 aaaaatctgg acagagttta cacagcagaa gatttcacag gatatttgtc aagcatgatc
     1921 tgcaatggtg tattggatac ttacgggaac aatttttcgg ttactgccgg agaaaagctc
     1981 tcggttctca tcggcacggg aaaagcatgg ctgaacggtc actatttcat caatgacact
     2041 gtatataccc tcgatttgcg gaaatatgca gatgaatccc tttcacgcta tgtgaccatc
     2101 ggcatcagct gtgatgtcag tgattccgtc cgtgcctgca aactggaagt aaagtccgga
     2161 acagcggcaa ctgcgccaac gattcctgtt tttgaaaata cggatacgaa aacatatctg
     2221 acccttgcgt cggttcgtct gaacggcggt gtaacgaaaa ttacggatgc caatatcaaa
     2281 gattatcgtg cagatgagaa gaaatgcggc tatgtgaagt gtattctggg aaaatgcagg
     2341 gtgtctgaaa tgcttgttgc tatgacggtt ttaacagaca gagttcaaga aatgactggt
     2401 aaactcgaca cgctgcaaag cactgtggat atgcttcagc taaaggtgga tgaccttaca
     2461 ggcgatgtaa ttgctgtcgg ttctcttggc aaggagattt actatgttct ctactggtgc
     2521 gtggcacggg agaaatgtat gattatgatt tggacaccaa cttatctccc ttgcgcggca
     2581 atagcgacat tcaagctgtt gtaattgatg agggaatcgt ttctgtaggt gattatgttt
     2641 ttgaaaattg caaaaacatg aaaaatatca cactcccgcc ctcgctgaca agtatcggac
     2701 aagcagcttt catgcaggga gataattata tcggtgaggt gtgtggttta acgaatattg
     2761 caattccgtc aaaagttaca agcatttcgg gcagtgcatt ctggggtgct gcaattgaaa
     2821 gccttgttat tcttgattca gttacggaaa ttggaaaata tg
//
LOCUS       DAWBGR010000239         2853 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8048_length_2853_cov_4.153324, whole genome shotgun sequence.
ACCESSION   DAWBGR010000239 DAWBGR010000000
VERSION     DAWBGR010000239.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8048_length_2853_cov_4.153324"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          683 a          711 c          853 g          606 t
ORIGIN      
        1 tgcaagcaga tggcgtgcaa agtcgccaga cgctctttgt gcggtattgc cctatggaaa
       61 ggaattacaa ctatggctta tgtaattggc gtggactgcg gtaccagcgg taccaaaact
      121 gtactgtttc gggaagacgg caccgttatg gcaagtgcga cggtagaata tcccatgtat
      181 cagccgaaaa acggctacgc agaacaggat ccggcagact ggaaagctgc aatgatccga
      241 accattcaga ccgttgtcac caaaagcggt gtggcaaaag aggagatcaa gggcattggc
      301 atttccggac agatgcatgg tctggtcatg ctggacaagg aaaatcaggt tctgcggaag
      361 tcgatcatct ggtgcgacca gcgtactgcc gcagaggtcg aggaaatgaa ccgtgttgtc
      421 ggtcgggaaa agctgatgga gatcactgcc aatccggcac tgaccggctg gactgccgca
      481 aagatcctgt gggtacggaa caacgaaccg gacgtatatg aaaagtgccg gcacattctt
      541 ctgccgaagg actatctgcg tctgatcctc accggcgaat atgccaccga agtttccgat
      601 gcttccggca tgcagctgct ggacatcgcc aaccgctgct ggtcggacga attgctgcac
      661 ctgcttcaga tcgacaagaa tctgctgggc aaggtgcacg agtcctgcga agtgaccgga
      721 acgctgactc gcgaaatggc agacacactg ggactgtgcg agggcacggt tgtagtcggc
      781 ggtgcaggcg ataacgctgc cgctgcggta ggtaccggcg ttgtcaggga cggcaaggcg
      841 ttcaccacga tcggtacctc cggcgtggta tttgctcaca cttcttctat ggcaatggac
      901 acaaagggca gagtacacac ctgctgtgca gccgttccca acagctggca cgttatgggt
      961 gtgacacagg gtgccggtct gtcgctgaag tggttccgtg acaacttctg tgccgcagaa
     1021 aagcagactg cttctctcat gggcgtggac gaatattacc tcatggacaa ggaagccgag
     1081 caggtgccgg taggggcaaa ccgcctgttg tatctgccgt acctcatggg cgaacggaca
     1141 ccgcatctgg atcccaacgc ccgcggggtt ttctttggac tgtccgcaat gcacaccaag
     1201 cgggatatgc tccgtgctgt gctggagggc gtaacgtatt ctctgcggga ctgcgtggaa
     1261 gtgttccgcg aaatgggcat ctccgtagac gatatgatgg catgcggcgg cggcggtact
     1321 tctccgctgt ggcggagcat gctggctgac ctgtacaatt gtccggtcaa gactgtggct
     1381 tccaaggaag ggccgtcttt gggcgtggca attctggctt ctgtgggtgc cggcatttat
     1441 ggcagtgtgc cggaagcatg cgatgccatt gtcaagaccg acaagacaca gaatccgcag
     1501 gcagaaaatg ttccggtata tgaatcctac taccagctgt atcgggagat ctatccggca
     1561 ttgaaagcac agtttgcgac actggcaggc ttgcagtaaa ctttgatata gtaaaaagaa
     1621 aggcagtgct gtttgggaaa tggcattgcc ttttttgaaa caggaggact caccatggaa
     1681 aaactcatct ccgttgccgt tatgcgtgac agcgatgccg ccgaaattgc cggaggaaca
     1741 gacagcaaaa tcttgatgta tcgtgcaggg ctggggatat tccgcagctt tgactggcac
     1801 ggcaaaatcg atattgtcac cggcagcggc aacaatgccg gtgacgggta tgtactggct
     1861 ctgctgctgc accggcacgg gattccctgc cgcatcgtcc gcaccagcga aaagtgttct
     1921 ccggacggta gctacttttt caccaagtgt caggaacggg ggattcccgt caccgcagaa
     1981 gcggactttt ccggcacaga ccagatcgtg gactgcctgc tgggaacggg gttccgcggg
     2041 gaacttcgcc cgaatctgcg gcaggtggtg gaacagatca accagtctgc tgcggcagtg
     2101 ctgagtgtgg acatcaacag cggcatgaac ggggacaccg gcacgggctt ttgtgtgctg
     2161 tctgaccgga cggtttccgt cggctgtctg aaatacgggc atctgctggg aatggcacag
     2221 ggaaagatcc ggagcctgta caactatgac atcgggattc cctgcaaggg cagctatctg
     2281 cctttgttta ccgcggacga ccgcacaccg gagaactggg actttacgcc ggaggacact
     2341 tttccacaaa ttctggacag tttccacatt cctgccggaa cgccggcaca gcgaatgcaa
     2401 aaactggcag aggcactgcg agaaaaaatc tgtttctgct ctgccagtga agcaggggaa
     2461 attccgtata cggcattgtg atcttatctt gcaattttcg ccgtggtgtg ttataataga
     2521 gctggaacaa gtcgttgttc ttcgctgcgg cgaatttcaa aaaaacggag cgtgaaaaca
     2581 tatgcagtat tcttatactc cacacggtgt ctgttcccgt gcgatcctga tcgatgcaga
     2641 agacggtgtt gtcaacaagg tgcagttcat tggcggctgc agcggcaaca cacagggcat
     2701 tgccgctttg gtagagggca tgccgataga cgaagtcgta aagcggctgg aaaacatcaa
     2761 gtgcggcagc cgtccgacct catgtccgga tcaactggca aaggcactga aaaaagtagc
     2821 agaggaacag taaaggcaat accgcacaaa gag
//
LOCUS       DAWBGR010000240         2845 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8074_length_2845_cov_3.469176, whole genome shotgun sequence.
ACCESSION   DAWBGR010000240 DAWBGR010000000
VERSION     DAWBGR010000240.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8074_length_2845_cov_3.469176"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          797 a          611 c          759 g          678 t
ORIGIN      
        1 acgaaaaatt tgacgacgca tctgtcgcac aatctttgtg cggtattgcc ttagctgatc
       61 gcctgcaaag ctgtcagact gattttatgc atacttttaa tttttttcaa acttttttag
      121 ttctatccat tttgaaaaac tggagttgta taatcaaagc ataaaggcaa taccgcacaa
      181 agctttacac agcgttttcc aaaggaatgc atggcatcgg aaaatttcga aaagcgtgtt
      241 ggattttgtc gaatcttgtc gaatgtaaga aatttagatg ttgacaagtc gaacgaaatg
      301 ttgtataata aagaacaaca gtaaaataca ggattgcagg gaggtgacag acaaatggat
      361 atgtcacgaa ttttgattgc caaaacggga aagactgcat cggagtggct cccgctggcg
      421 gtgcatctgg aagatacggc cgggatcatg caatgtatga ttcgggatat gattccgccg
      481 tcttttccgg aatcctgtgc cctctctccg gaggcgtttg aaaaaacagc agtttttttg
      541 gcatatgtgc acgacattgg aaaggcaatg cttgtgttcc aatataagat cagcagaaat
      601 cttccggaga tgcggagccg tctggagcat tttcagctgc aatttcctga aagctatgaa
      661 aaggataaaa tcaacgtgat cccccatgca caggcagggg aagaaattct ggtcacgctg
      721 gggtgccctg aaagtgtcgc cgctgttgca ggagcacatc atggcatgcc gtcagagcac
      781 atacagaaga atctgatgca gccgcgaaga aatatcaaat tctacgagaa ttactatggc
      841 tatgcagagg gaaatgtatc tctgcttacc gaaacctgga atcacatttt acaaagggcg
      901 ttgcaaattg cccggatcga ttcggtacag gagcttccgg tactgacgaa accggcacag
      961 atgctgttgt ccggtctgct gatcatggcg gactggatcg cgagcaacac agattatttc
     1021 ccgctgattc ccacggacga ggacattcct gaaaatccgg aaagaatcgc tgatgcatgg
     1081 gaaacgatcc aattccccga aatgtggaag tctgcacgat tggactattc cgatgcggcg
     1141 tttcgcaaaa acttttcgtt tgcaccgcgt ctggtgcaga cagagtttct gaacgtgata
     1201 aagaacatag agtcaccggg aatctgtatt cttgaggctc ccatggggtg cggaaagacg
     1261 gaggctgccc ttgccggaac agaaatcctc gctgcgaaat gccggaaaaa cggtatgttt
     1321 ttcgggctgc cgacacaggc aactgcgaac ggcatttttc cccgcatgat gcagtggggc
     1381 gaaaagcagt cccagaattt ctaccattcc attcagctga aacatggcag ttccgctttg
     1441 aatacctgtt ttcagaaaat tcagagaggg ataccggaac aggaaacgga cagcggactg
     1501 atcgtgcaca gctggttctg cgataacaag aaagcctgtc ttgccgactt tgtcgttgca
     1561 acggtggatc agatgctgat gatggcattg aagcgaaagc atgtgatgct tttgcacgtg
     1621 ggcttgtctg aaaaagtagt cgtgatcgat gaagtgcatg catatgatgc gtacatgaac
     1681 gaatatctgg aaatggctct ccaatggctg gggtactata aaacgcctgt gatcctgctg
     1741 tccgcgacgc ttccggcaag ccggagaatg tcgctggttc gtgcatacct cggaatcaga
     1801 gaatccgaaa aggcctttga aaacgaaatg gggtatccgc ttctgacgtg gacggacgga
     1861 actgagatcc ggcaaaaaag attgacgtat cagggagcac atacgcatgt tcagatgcac
     1921 ccatgtacag aagatgctgt tctggcagtg gttcagaatg ttgtagagca tggcggctgc
     1981 gttggcgtga tcgtgaatac agtgaaacgt gcacagactt ttgcggaaga aatacgaaat
     2041 cagacccatg caaaagtgct tttgtatcat gcacagttta tattccccga cagaacatta
     2101 aaagagcagg ctcttttgaa aactgttggg aaagacagca gtgcagaggt tcgtcgcgga
     2161 accgttgtgg tcgggacaca ggtcttggag cagtcgctgg acattgactt cgatttgctg
     2221 atcacggaca tttgccctat ggacttgctt ttgcagagaa tcggcagact gcaccggcat
     2281 gcctttcggg aacaaagacc ggaagttgtg aaaactccgg tctgctatgt catcatggac
     2341 gaactgcatg gggaaaatac cgcgtctaaa aagatatacg gcgatttcct gctgcatcga
     2401 acagaggaaa acctgcctga aagcattgtg cttccggacg atatatcgcc ccttgtgcag
     2461 gcagtctata cgttttccga ggacgatgcg atgtatcaga catatcataa tcaacaggaa
     2521 atccaaaaac ggcgtgcacg ctgtttccgg atcggaaaac ctacgggaaa agatatacac
     2581 cggctgttgt cccgcgatat agaagataaa aatgaatgtg aagtggaggc agctgttcgg
     2641 gacgggattt cttccataga agtgctgctg atgcggcgta tgaaagacgg cagcatttgc
     2701 ttcttgccca atcagcatgg cggcaggaaa acagaggcgt ttccggacga ggcagagtgt
     2761 cgagaaattg cagagcagaa gctgcggctg ccgacagtgt tcagccagaa gtggagcatc
     2821 gaccggacga ttcacgaact ggaaa
//
LOCUS       DAWBGR010000241         2839 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8098_length_2839_cov_4.316092, whole genome shotgun sequence.
ACCESSION   DAWBGR010000241 DAWBGR010000000
VERSION     DAWBGR010000241.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8098_length_2839_cov_4.316092"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          626 a          711 c          665 g          837 t
ORIGIN      
        1 catcttacgc acaagcttta ttcagcgttt cctaaagtca ttttcggcga tcttctgatg
       61 tggaaaggtg atgaaaaatc atgggaatta tgggcgtgat cgtgctgatc attctggtga
      121 tcggcgttac caaatacggg atacgataac attttaaatc ggggaggatt ttgaacatgg
      181 taaaactgat tctgctggca cttttcgtat gggtcatctg gctcacactc cggaaaattt
      241 tcagaggata ctaaaggcaa taccgcataa agcccgcctg acggctttgc acgcacaagc
      301 tttatgcagt gtttccctga ttcctgcgaa cacaaggcga caaaaagtcc tgctctcctt
      361 ccacggagaa caggactttt ttcactgctt cgcagcgtat atccgttgct ttattcgccc
      421 ttgttcggca gtgtgtgaat gatcgatacc aggaacttca gcagtactac ggcatcattg
      481 ctgtctactt cgccgctttc gtcgcagtca gcgtttgcct gctgctgtgc agaaagagat
      541 accgcatttg cagccgcctt gttcagcaga accgcatcag tgatgtctac tctgccgtcc
      601 agattgatgt cgccgtacaa tgtagcagct tccactgcac cctttgtggt aactgtggtt
      661 tcacttgccg ttgtagcggt ggtgtctgca ctggtttctg ttgcagaaga ttctacagtc
      721 gtttctgtgc tttcttcggt atccgcagta gaaacggtgg tttccacagt cgtttctgcg
      781 gtatcttctg tggtggttac tacagaaaaa taagtagttg ctgtggtttc cgtttccgta
      841 gtagtttctg ctgtcgtatc agttgttgca tctgttgtta cagttgtgcc cggttctgca
      901 ttgccataca gatctttcgg cagttcgtcc agtgtgatgc agtaatcgct ggtgtacatc
      961 ttcttcagct gagccgcatc gttatactgg ctggacatca ggtgatcgcc gtaccacggg
     1021 cagaaataca gccagccggc attttcaatg accatgtttt cctcagacgg aatggagtca
     1081 ttttctgcca tagcaaccat tttcttgccg ctggtcagtt catacagata gttgaagatc
     1141 ggagaaatgg cactcaggtt cggtgtgccg gagctctgac cgtcgtcgcg gttaaaatcg
     1201 cagttatact tgtcgtatgc cacgatgtct acatagtcat cacccggata ccatgtatca
     1261 gagtttgcat agttgtagct gttgtattcc cagatgatgt tgtgcagacc atattcttcg
     1321 gtcagcgtgg tgtacagcag cttccacagt tccttgtata cttctgctcc gcggtcgccc
     1381 caccagaacc aagaagtacc gtcgctgtag ttaccttcgt tgccctgtgc ttcatgcagt
     1441 ggacggagaa tgatcggcac gttggcatcc tgtagcttct ggagctgttc tgccagatcc
     1501 ttcatagctt cctggaagta ctcgtattcc tttgtgcctt ccttgactgc gtttgctgtg
     1561 ttgaaagtgc tgttcgatgt ctgatagttc ttatacgtgc atttcttcca gtcaacctcg
     1621 tcacccagcg tgtatttgtc aaaatcgatc ggcacattga tatgccacga tgcagtgacg
     1681 ataccattct gttcttttgc ccatttgatt gcacgttccg ttgtgccgtc ttcccagcca
     1741 tacagcgggt tgtagttcat aaagtcaaaa ccgcggatcg ccggcagctt tccggtattg
     1801 tccttaatgt agttgaattc cttttcggaa tcgccgtcat tcccgccgcc gtaaatttcc
     1861 tgctgaccag aaatgatgtg agaaccatat acgctcttga ggtaggacat cagagccttg
     1921 gtttccttgg ttgccttggg gtctaccggt gttgccgttg ccttttcata ttccggcttc
     1981 ggtgtctgtg tcagagtcat gttgtcgtac aggcaatagc cccagccgcc gccgaatgta
     2041 atggtgtttt cacccttggt cagcttcaga tcgccccagt tgtaatcgtt ccagtcatct
     2101 gtatgtgcga tcttgacggt cttggtagcg gtactgtcgt cagaacgtgt aacagtcacc
     2161 tgtgcttccc gaatgtcgct ggaagaagtt cccagataca taatgcatct ggtcatcagc
     2221 cgatacatgc cagtttccgg tgcatcaatg gtaaagctca tggtgccgga actggtgacc
     2281 cagacaaatc cggcaccggt ataacccgga tattcatcat tatagacttt tgttgcaacc
     2341 tgtgcatcac ttgacaaagt cagatcctca cactccgcag agaacagcgg atccgcagca
     2401 gataccacag ccgccggcac tgcctgtgcc atgcccagac aaagtgctgt cgctacgccg
     2461 gcactgccca gacgtttcca aaaattcttc attgctccat cttcctttct ggttacagag
     2521 tttatcactc aacctgcagc ggcagtattg cacaaaacag ccgctgaatt tcatagaaca
     2581 attatacacc ctttccgaaa atttgtcaat agtcacaaaa cggtttctgg aaaagttttt
     2641 tggactgggc atttttccgt agtcgaattg acaaggcaaa tcgaaggtgt tataattatt
     2701 aagaaaacac tgcataaagc ccgcctgacg gctttgcacg caatctgctt gcagattttc
     2761 cgtcagattg cccagaaatc ccttgccaga catacagtct gcctgcggaa tttctgggca
     2821 atctgccgaa aaatctgac
//
LOCUS       DAWBGR010000242         2829 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8121_length_2829_cov_4.339221, whole genome shotgun sequence.
ACCESSION   DAWBGR010000242 DAWBGR010000000
VERSION     DAWBGR010000242.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8121_length_2829_cov_4.339221"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          635 a          839 c          640 g          715 t
ORIGIN      
        1 tggcgaaaaa tttgacgacg catctgtcgc acaatctttg tgcggtattg cctaacaaac
       61 cacatacaaa aagccgtatc ggtaattcca ttccgatacg gcttttttct gtccgttcaa
      121 aaattcatga ttgtaaagcg gtctgcgtgt tgttcttctg ttacgccacc agcatctccc
      181 acgtggtggg aatccccgca ctgttccgtg caatatacaa cagcacatag aacacgtccg
      241 tagaatcgac tgcaccgctg ccgtcaatgt ccgccgcttt ttcctgtgcc ggtgtccagc
      301 cggaatccat gcccactgca ttctgtgcaa cgcccagcag gatgtaaaac acgtccgtag
      361 aatccacgct tccgttgttg tccaagtcgg caagaagcag ttcttcaagg atactattat
      421 aatgtattgt cgcctgctcc agcagttcat tccctgattc gatctggaca ttgctccact
      481 cgtcttccga catgacagtg tacactgccg acagtttcgt acagtcggaa aaggcagatt
      541 ccccgatttt tgccagattg ccctgtactg tcagcgtttc cagaccagaa cactgccaga
      601 aagcacccca atcaattttc gtcacacttg ccggcagcat gatcttctgc aaatttccgc
      661 agttgtaaaa ggcaaagttc ccgatttccg tcacaccgtc cggcacttct accgctgtac
      721 cagagcacgt tgtcgcgtca atcaaaatat ggttcacctg taccagtgga ttctccgtct
      781 gctgattttt cagccacgcc gtatcgcaaa acgcatctaa tccgattttc tgtaaggtca
      841 ccggaatttc caattccgcc agcaagccac aggaacgaaa gcaattatct cctacagcag
      901 ccagattttt cggcagcgtc acatgctgta acttatgaca actccaaaac gcattctttc
      961 cgatcgtctg taccgtgtcc ggtatctcca cactggtcaa attttcacaa aattgaaacg
     1021 catcttctgc aatttccgtc actggttttc cggcaatttt ttccggtatt accacttttt
     1081 ccgccgtttc ctcacatttc gtgataactg ccccgtcaga ttccaagata tacgtcaacg
     1141 ccccaacagt tcccgtttcc tctgctgccg ctgccgaaaa tgccggcaga agcggctgca
     1201 cctgcggcat actgataaca cagagcattc cggcaagcac tgctgccagc ggttttttcc
     1261 aaattttcat gtcattctcc ttgtcctgtt gtttctctcg catctttctc cagcaccgaa
     1321 aacgcctgcc ggagattccg cacgccggca atgcgaatct gataggagcc gcctttcagt
     1381 tcgtgcaggc actgatgcgg cacaatgcac cgcgtaaagc ccatgcgttc cgcctcctgc
     1441 acccgccgcc gaatgtggga aatgccacgc acttcgccgc ccagtccaac ttcgccaaag
     1501 gcaaccgtcc gttccgagat cggcttgtcc accagcgagg agtacagaca cagtgccacc
     1561 gccaagtccc ctgccggttc gtccagctgg aatccgccga ctacattcag gtacacgtcc
     1621 agcgttccga aaaacatgcc cagatgtttt tccagtaccg cgatcaggat caccatgcgg
     1681 ctatagtcaa acccctgcgt ctgccgccgc ggtgcagaaa agccgctttt ggtggcaagc
     1741 acctgaattt ccgccagaat cggacgggag ccctccatca tacaggtaac acaggtgccg
     1801 gaaacgccgt gaggtctgcc gtccagcagc attgccgaag ggttgggcac ttcgtgcagt
     1861 ccgctgtcct gcatctcgaa cacgccgatc tcattggtag aaccataccg gtttttcaca
     1921 gcacgcagca gccggtagga caggttccgt tctccctcga aatacagcac ggtgtccaca
     1981 atatgttcca tgaccttcgg accggcaatg gctccgtcct tgttgacatg ccccacgatc
     2041 cacaccggaa tgtccagctg tttcgccgtg tgcataaaca ggttggtgca ctcccgcacc
     2101 tgtgtcaggc tgccggcact ggaggaaatt tcctccattc gcatggtctg aatggagtcg
     2161 ataatgacaa tatcggggcg gctggcggaa atggtgtcgc agatgctctg ggcatcggtg
     2221 actgtcagca gaaacagatt tttcgttgtc acgcccagac gctccgcccg cagcttgatc
     2281 tgtttggcgg attcctcacc ggaaacatac agcacagtgt gttcctttcc gaagtactgg
     2341 cagatttgca gcagcagtgt actttttccg atacccggct cgccgcccag cagcacaatg
     2401 gagccacgca ccagtccgcc gcccagcacg cgatccagtt cgcctacgcc ggtatgataa
     2461 cggacatcag ctgtggtgtc cacttcttcc agttcctgta ttttatccga caagtccacc
     2521 ggtttccggc ggttgttgcc caccgcctgt gtgggcagcg ggacatcttc ttccaacgtg
     2581 ttccacgagc cgcaggaggg acacttgccg ttccacttgg cactttcgta gccgcatgct
     2641 gtgcagcggt atatgatttt aggctttgcc atagaggttc tcctttttgt tgtgttattt
     2701 gtattatacc atgatgcgga tacaagtgca agaggataaa aaataaagac cttaacttgc
     2761 atggtacaag ccaaagtctt ttaagaaaca aattaaggtt gccgttccta ttattgttct
     2821 gtattctta
//
LOCUS       DAWBGR010000243         2820 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8152_length_2820_cov_4.506691, whole genome shotgun sequence.
ACCESSION   DAWBGR010000243 DAWBGR010000000
VERSION     DAWBGR010000243.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8152_length_2820_cov_4.506691"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          743 a          712 c          731 g          634 t
ORIGIN      
        1 ttgcaagcag atggcgtgca aagtcgccag acgctctttg tgcggtattg cccaagagct
       61 ttctgatgaa ttccgtggca ctatcaaaaa atctgtgtaa aattgtaaaa aagtacttgt
      121 attttgcttg ttcctatgct ataataaaag tactaaccgg acgtgagggg ggtatacctg
      181 acgtttcggt aatcttacta ttttacgaaa aggagttccc tcatgaaatt tggctatttt
      241 gatgacgcaa acaaagaata cgtcattcag aatccgaaga ctccgtaccc gtggatcaac
      301 tacctcggta cacaggagtt cttctctctg atctctaaca ccgcaggcgg ctatcacttc
      361 tataaagatg cccgtcttcg cagaatcacc cgttaccgtt acaataacgt accggtagac
      421 atgggcggtc gctacttcta catcaaggac ggcgaaaccg tatggtcgcc gggctggtcg
      481 ccggtaaaga cagatctgga cgcttacacc tgccgtcacg gcatgggcta caccgtgatc
      541 tccggtaaga agaacgacat ctccgcagaa gtgactttct tcgtacccca gaactacaac
      601 ggcgagatcc agagtctggt tctgaagaac gagggcagcg aggcaaagac gatcaagctg
      661 ttctccttca ttgaatggtg tctgtggaac gcacaggacg actccaccaa cttccagaga
      721 aacttcaaca ccggtgaagt ggaaatcgaa ggctctacca ttttccacaa gaccgaatat
      781 aaggaacgcc gcaaccactt cgcattctac actgtaaacg atgcaatcga cggttacgac
      841 accgatcggg aaagcttcct cggtctgtac aacggcttcg agaatccgca ggtcgttgca
      901 gcaggcaagg cttccaactc cgtagcagac ggctggtcgc cggtggcttc ccactataag
      961 gaaatcacac tggctccggg cgaatccaag cagctgatct tcattctggg ttatgtagaa
     1021 aacccgaagg atcagaagtg gaacgctgac ggcagcatga acaagtcccg tgcatacgag
     1081 atgatcgaac agtacaacac accggaaaag gttgctgctg cactggctga actgaaggca
     1141 atgtgggacg ctctgctgtc caagtacagc gtcaagacac cggacgacaa gatgaaccgc
     1201 atggtcaaca tctggaacca gtatcagtgc atggttacct ttaacatgtc ccgttccgct
     1261 tcttacttcg aaagcggtat cggccgtggt atgggcttcc gtgactccaa tcaggacctg
     1321 ctgggcttcg tacaccagat tccggacaga gcaagggagc gtctgatcga tctggcttcc
     1381 acacagctgg aagacggcgg ctgctatcac cagtatcagc ctctgaccaa gaagggcaac
     1441 aacgagatcg gcggcgactt ctctgacgac ccgctgtgga tgatcttgtc tgtagctgca
     1501 tacatcaagg aatccggtga ctactccatt ctggacgcta tggtgccgta tgacaacgac
     1561 gaatccaagg caaagaccat gatggatcat ctggaaaaga gcttcttcca tgtggtagaa
     1621 aacgttggtc ctcacggact gccgctggca atgcgtgcag actggaacga ctgcatcaac
     1681 ctgtcctgct tctctgacga gccgggcgaa tccttccaga cctctacttc tccgaagtac
     1741 ggcgaggata agtattccaa ggttgcagaa tctgtcatgg ttgctgctct gttcacctat
     1801 gcaggtccgg aatatgtggc aatgtgcaag aagaagggtg acgaggctgg tgctgccaag
     1861 gcacaggctg ccatcgacca gatgaagaag aatatcatgg aatacggctg ggacggcgaa
     1921 tggttcctgc gtgcatatga tgacttcggc aagaagatgg gctctcacga atgtgaagaa
     1981 ggcaagatct tcatcgaacc gcagggcttc gcggttatgg gcggcgtcgg taaggaaacc
     2041 ggtgcagacc tgaaggctct ggagagcgta gacaagtacc tgaactctac acacggtctg
     2101 gttctgaacc agccggcatt caccaagtac tacatcgaat acggtgagat ctctacttat
     2161 ccggcaggct acaaggaaaa tgctggtatc ttctgccaca acaatgcatg gatcatctgt
     2221 gcagaagcat ttgttggtca cggcgataag gcattcgaat actacagcaa gatcgctccg
     2281 gcatatcgtg aagaaaagta ctccgacctg caccgcaccg agccgtatgt atacgctcag
     2341 atgatcgcag gtaaggacgc aaagcgtcac ggcgaagcaa agaacagctg gctgaccggt
     2401 actgctgcat ggaacttcgt agcagtttcc cagtacattc tgggtgtcat tccggattgg
     2461 gacggtctga agatcgatcc gtcgatcccc cacgaatggg acggcttcac catttccaga
     2521 cagttccgcg gtgcaaccta tgatgtcacc gtcaagaatc cgaaccacgt ttgcaagggc
     2581 gttgtatcgg taactgtaga cggcaaggca atcgagggca acgtactgcc ggtattcagc
     2641 gacggcaaga gccacgcagt agaagtggtt atgggctaag ctgagtttcc ctgagataat
     2701 atatccctat ttccttttca aaaagaggga ctgccatttt tcgtggcagt ccctctttgt
     2761 ttttaggcaa taccgcacaa agattgtgcg acagatgcgt cgtcaaattt ttcgtcagat
//
LOCUS       DAWBGR010000244         2811 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8180_length_2811_cov_3.601234, whole genome shotgun sequence.
ACCESSION   DAWBGR010000244 DAWBGR010000000
VERSION     DAWBGR010000244.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8180_length_2811_cov_3.601234"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          669 a          749 c          579 g          814 t
ORIGIN      
        1 ggtcgaccac ggtcgctgcc gtgaaccgtc catgtgcttt gcaccgcaca cacagtggat
       61 gcttccgcag gtacgccttg ctgagcctct gccacctgct gccgtagcca cgcttggcgg
      121 cagatggtcg gtctgggtgc aggggctgat gctctgcaca gtacaagccg tctgtcagat
      181 tgggacagcc gggatgctta catggtttca gtgccttcct cggcataagg ttcacctccg
      241 gatacaacga aagcccatgc ggaacaccac agggctttcg gtcagttttc tatgatatta
      301 ttatatcatg tttattcagc aatttcgtcc atcaaattac ctcatgcctt tccgtacaaa
      361 agcaaagcaa gatgctgcac tgcacgattt ttcttattgt aagctgtaga acgttcgata
      421 tgcaggtgct cacagatatt gtaaacagca tcaatctgtt tttcttctcc tccatagaac
      481 tgttccagca cataccgttc atcctccgac aggctgtccc atgcaggctg aaaccattcc
      541 atgtactcct ttgcctgacg ataccgttcc cgcagcacat cgatttcgtc aatggcagtg
      601 atgattcgca tttcgccgga ctgcgggttc ggactgccgc ccggcatatc tgtaaatgcc
      661 ggactgccaa gggttgtggt gtcttcatgc acctgtgcga tttcttcgtc tgtgtgtgca
      721 aggatgtaag ccatgctgct gtaatccttc agtgcgttta cagcggcact ccgtttgtct
      781 aagtactgcc aaatgatatt catctgctac ctccaagttc tgctttgact gcctgcatca
      841 aagcggtctg ggtttgttcc ttccgggtca gggctttcag gatacgttcg tcaatcgtac
      901 ctttggtgat gagatgttga atgacaaccg tttcagattg ttgtccctgt cgccatagtc
      961 tggcgttggt ctgctggtag agttccagac tccaagtcag tccaaaccaa atcaggtgag
     1021 aaccgcctgc ttgcaaattc aggccgtgtc cggcagcggc tgggtgcagc agaccaactt
     1081 gcagcttccc agcattccag ttccgaatac tgtcagaaga ctggatttcc tgatacgaaa
     1141 cccgaagctg ttgcagtcgc tcttgaatcc gctccaaatc atgcttgaac caatacgcta
     1201 tcagaacggg cttgccgttg gcggcctcta tcaaatcttc cagtgcatcc agctttcggc
     1261 tgtgaatggg aatcaccgct ccggtgtcgt catacactgc accatttgcc agctgggaaa
     1321 gtttgttgga tagacttgca gcgttggcgg cggtaatctc gccatccggc aagtctaaca
     1381 ccaattcttg tttcaattgc ttgtatcgct ctctttcttg cttagaaaga cgaactggaa
     1441 cttctgtcaa caaaagttct ggcatttgca aataatcaat cgctttcatg gaaatggtga
     1501 tgtctgaaat tttatcatag atttgtttct ctgcctgcgg caaaggcttg taagaataaa
     1561 ccaccatccc atttcgttta tccggctgaa aataggctgt tcgatattgt ccaataaatc
     1621 ttccaagccg ctgtcccata tccagcaaac gaaactctgc ccataaatcc atcaaaccat
     1681 tactggacgg tgttcccgtt aagccaacaa tgcgtttcac ctttggtcga actttcatca
     1741 gggctttgaa tcgttttgtc tgatggttct taaaaccgga taactcatca atcaccaaca
     1801 tatcgaaatc aaacggaata ctgctttcct ctaccagcca actgatattc tcacgattca
     1861 aaatgcaaat atccgtcctt gcatgcaggg cttgtctgcg ttctgcggat gtcccaactg
     1921 ctacactgta tttcagatgc ttcaaatgtt cccacttttc aatttctgct gaccaagtat
     1981 cccgtgccac acgaaggggt gcaatcacta aaacacggcg gatttcaaaa cgatcaaaca
     2041 acaactcgtt gattgctgtc aatgttgtga cagtcttccc taatcccata tccagtaata
     2101 atgcagctac aggatgctcc gtcaaaaact gaatcgcata ttgttgatag tcgtgcggaa
     2161 tgaacttcac ggtgtttcgc ctccaacttc atccaaaatg gggcggatca aatccagacg
     2221 atccagacaa tacacggaaa agcccactct ctcaagctgt tgttttctcc ggatttgtaa
     2281 cgcccgcatc ttctcacccg gagcctttac ttccacaaac gcaatttttc cacccggcat
     2341 caatacgatt cgatccggca ctccatccgt tcctggactg gtaaatttcc aacaaagacc
     2401 tcctctggac tgcacctctt gtaccaaccg gctttcaatc attttttcac gcattttagc
     2461 caccttttca agttttttct tttttggggt gatggtctgt tacggtcaat atataaaacc
     2521 ccttttaggc tgaaaatttg gtaaaaatta cctataagaa gagtttacga aatgaccctc
     2581 tccgaccatc acccctgccc attattccaa aaattctgac ttgattttta agccgtaaac
     2641 gatgacacct ttcttggttc tctttcgttc aaagcctaca ttttcaagcc ccgtgtaaaa
     2701 gtccgttgta ctccttgtgt actctccatt tctggcacag taagcacgat attcctgata
     2761 cagttcgcct gacttttgct gataggtctt atctacctca cagcagtctt c
//
LOCUS       DAWBGR010000245         2766 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8348_length_2766_cov_3.589819, whole genome shotgun sequence.
ACCESSION   DAWBGR010000245 DAWBGR010000000
VERSION     DAWBGR010000245.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8348_length_2766_cov_3.589819"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          711 a          643 c          859 g          553 t
ORIGIN      
        1 cgtgcggtgt tgccttaggg ggcaacactg cataaagctt gtgcgtaaga tgcgtcgtca
       61 gatttctggg caatatgacg gaaaatctgc aagcagattg cgtgcaaagc tgtcaggcgt
      121 gctttatgca gtatttcctt agacaggagg aattgcaaat gcagaaacag gaacaaaaca
      181 taaagacgat gagcccggac agtaacgagg cactgtgcat tcggatacag cagggcgacg
      241 aacaggcaaa gagccagcta ttgcagcaga atgaaggtat gatccatgcg gttgcatggc
      301 gggaacgccg gcggtatgcg tatcttgcac tggaactgga agacctctgg gcagcgggac
      361 agctgggcat gctccgtgcg gcaaggatgt tccgtccgga aactggaaac cagtttgcca
      421 cctatgcgtg gcctcacgtg cggcaggcaa tccagcggga aatcatttcc ggcggaacca
      481 ttatccggat ccctgtgcac ctgcacgaca gaatgcacaa gctgtccgta tatcgggaac
      541 caaacggcag tgtgaactat gaagcgttgg cagagcgtgt taccgcagaa gaacgaaccg
      601 gacacggcat gacggcttcg gaggtcaagg agtttctgtg tcgggtagaa cccatggtgt
      661 ccctgcggag cctgaacgaa atgctgaagc tggacggaga aaccgaacgg caggagatgc
      721 tcgccgagcc agatgccaga acgccggacg aagaagtgga gaaccatttg tttctggagc
      781 agtgtctgga acagctgaat ccgcgggaac gggaagtgat cttcatgcgg tacggcttgc
      841 agaacaacga gagcatgaca ctgctggagg tggggcagaa gctgcatatc accaaagaac
      901 gggttcgcca gctggaacag cgggcaatgg aaaaaatgcg ggattggctc agtgcatgag
      961 atatggggat attgtcctta acaaagaatt tctgtaatga tgaaactggg aaaacgcttt
     1021 acaccatgtt ttccgttcga caaaaatcct gtacaggttt gtggtatact gttttcggag
     1081 ggagtgattt ctcacaaaac ggcgaattcg ctggggcgaa gccgtcggga aaggaaatgg
     1141 tcatatggca gtagaactgc aaacgcagga aaaaacactg atcgcgattc tcagcggcga
     1201 gatcgatcac cactgtgccc agccgatgcg tgcggagatc gatgcaaaaa tcgacgatct
     1261 tcagccgtcc gtgctggtat tggattttgc cggtgtgaca tttatggaca gctccggtat
     1321 cgggctgatt atgggcagac accggacaat ggaggcgatc ggcggaagcg tgatcgtgcg
     1381 ggatccgccg ccccatatcc gccgggtcat tcggctgtcc ggcatggagc gaatcgcaca
     1441 gattcagata cagaaaccgt cctgccgcga gggggatttt ggcatcggag gaggtgaaaa
     1501 tgcatgaaag tggtaaatgc agtaaaattc attttccccg gcatctccga aaacgaacgc
     1561 accgcacggg cagtggtttc cgcttttctg gtacagctgg atcctacggt agaggaactg
     1621 gcagatattc gcacggcagt ttccgaggcg gtgaccaatg ccattgtcca cggctaccgg
     1681 aacagcaggg gtgacgtgga attacatatg aagctgctgg aagatcggga ggtctatctg
     1741 cgaatcaaag atcacggctg cggcattgcg gacatcacac aggcgatgca gcctctgttt
     1801 accacggcac cggaagaaga acgtgccgga ctgggctttg cggtcatgga aagcttcatg
     1861 gacaagctgc aagtcaaaag tgtaccggaa aagggaacga ctgtgaccat gcggaaaaag
     1921 ctggggagcc tgccatgtcg ggtgaagtaa caacgccccg tgcagaggaa catctgggac
     1981 tggtgcacct ttgtgccaac cgctttcgga atcggggaat cgattatgac gagttgtact
     2041 ccgcaggctg cgtgggcttg ctgaaagctg taaaggcgtt cgactgtacc cgcggtgtgc
     2101 agttttccac ttacgcagtg ccggtgattc tgggcgagat caagcggctg tttcgggacg
     2161 gcggcattgt acacatcagc cgcagtctgc gggaacgggc aacaaggctg caaaagttac
     2221 aggaaacatt tgagcagcag cacgggcgtt cgcctacggt ttcagagttg agtgccctga
     2281 gcggggactc tgtggaggaa tgcggggaag cactgtgcgt aacgcagaca cccctttccc
     2341 tgacacggga cagcgacgag gacgacgacg ggcagttcga cattccggtg gaagcccccg
     2401 accggcaaat tggcgatctg ctgtccctgc ggcagctgat gggggcattg ccggaaaaag
     2461 accgcatgct gttggaactg cggtattttc gggaactgac acagagcaaa acggcggcga
     2521 tcctgggaat gacacaggtg caggtgtcgc ggcgggagaa aaaactgctg acgcagctgc
     2581 ggcaggagtt gttgtcttag aattgctgtg attggaaaga ggactgtgaa ggtcctcttt
     2641 tttgttggaa aaacaaccaa aacaaaagcg tgcttcattc accggacagg ctcttaggca
     2701 gcactttgat attaaggaaa cactgaataa agcacgcctg acggctttgc acgcaatctg
     2761 cttgca
//
LOCUS       DAWBGR010000246         2766 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8347_length_2766_cov_3.969015, whole genome shotgun sequence.
ACCESSION   DAWBGR010000246 DAWBGR010000000
VERSION     DAWBGR010000246.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8347_length_2766_cov_3.969015"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          656 a          742 c          631 g          737 t
ORIGIN      
        1 aatttgcaag cagatggcgt gcaaagtcgc cagacgctct ttgtgcggtg ttgcccaaga
       61 aaaacgctgc cgccggttat accatcagca tgcggaacat ttcgatcacc tgttcctgcg
      121 ttgcctctct ggggttgccc ggatagcagg catctgccag agcatctttt gccagctgct
      181 ccagatcttc ttccctgatg cgttcgttct tttccggaat gcctacgtcc ttggacagct
      241 gacgaactgc cgcaattgcc gcatcacggt attcctcctg tgtcatggtt tcagtaccct
      301 ctacgcccat tgcctttgca atcgcacgat accggtcacc ggtgtagtcc cgattgaact
      361 ccatggagat cggcagcagc attgcacaag ccacaccgtg cggggtgtca tagtgtgcac
      421 tcaatgtatg tgccatagag tggtcgatcc ccagccctac attggaaaag cccatgccgg
      481 caatgtactg tgccagagcc atgccctctc tgccgctggg cttattggct acagcatcac
      541 ggagattctc cgagatcagg cgaatggctt ccatgtggaa catgtccgga atctcccatg
      601 ctgccttggt ggtgtatccc tcaatggcat gggtcagtgc gtccataccg gttgcggcag
      661 tcagtccctt gggcatggag gacatcatat ccggatccac aatggcaaca gccggaatat
      721 cattggtatc cacgcagacg aacttccgct gtttttccac atctgtgatg acatagttga
      781 tcgttacttc tgcggcagtg ccggcagttg tcggaatcgc aatagtggga acggcgtggt
      841 tcttcgtcgg agcaacaccc tccagcgaac gcacatctgc gaattccgga ttggtaatga
      901 taatgccgat cgcctttgcc gtgtccatag aggagccgcc gccaatggca acaatggcat
      961 ctgctccgca agcctgaaaa gccgttacac cctcctgcac gttctgaatg gtgggattcg
     1021 gcttgatctt cgagtaaacc gtgtatgcaa tgccggcatt gtccaaaatg tctgtcacct
     1081 ttgccgtcac gccaaattgc agcagatccg gatctgttgc aataaacagg tgccggtagc
     1141 cgttgctttt tacttcactg acgatttcct gaatcgcacc ttttccatga taagaaatgt
     1201 tgttcaatac aatacgattt gccataatgc atgcacctca cgataaaatt tattggtact
     1261 tccattttcg aagcacaagc atattataca tcatttttca gtaaatgtca atatgtaatg
     1321 tgcaatatca caaaaatgta tctgtatcca atggtatggg cgaattgaaa gcgtaaaatg
     1381 ctgctatttt cggatcatgt tcattcacaa tatcttcctc tataccgtaa tgctgcatgc
     1441 cgtattgcag cagctgcctg cccgaatctt ttccgtgttc ctccggcaga aggaaaaaca
     1501 ttaccagccg ctttccggca gtgcccataa aaccgacagg ctcccctgat gcggattcct
     1561 ttccgtttgc tttgcgtgtc tgtaagttcc cgcaatccgt gaaactccta tggcaatacc
     1621 gcacaaagat tgtgcggtat tgccatataa aaaaagccgc ctctgcggac agaggtgact
     1681 tctgagaaat gatttcagtt actttttctt gtttttcgtg tcctgccgga gcagctgcat
     1741 tcgcagtgcc aagcaagtga ctgccagaat cactgcaccg ccgacaacga tccagcccac
     1801 gactacattc cgcatatggt aaatatcaga atagggcact gtcgccagaa tgatgtaatc
     1861 gccgctggtc tgatagtgca ccatttccgt tttgccgtct gcatctttca cattaaattt
     1921 gcccttgttc cgctgtaact gttccttgtc gtagtctgct gtcttgccga tcaggtctgt
     1981 attggtgcag ctgatatatt tcagcgtgtc ggtttctaga attgccgttg taccggaaga
     2041 atacagcggc atatccgaag caatgtttgc ccagtctgcg tcctgtaaca gagataccac
     2101 ggaatccgca gaaaacgtga tctgtaccag accgtttccg ccgtccagtg aagatgccgc
     2161 cacaatggtg ggcgtatccg aatccagcag aaacagaaca tcggtataga catcatctgt
     2221 caggtgcgaa cgaaactctt cccgaatgga gttccccgca ccggacgggt ctgtactggc
     2281 gatcacattg ccctcatgat cagaaatact aatcatgtct gcattgacgg tcacgcgaag
     2341 ttcctccagt gtccggtcat cggtaataaa ggaactgtcc tgcgacagca gcatggcgat
     2401 agttctggtc ttggcagaat agtcctcttt gaattcctct gtcagcagtt ccgttgcatt
     2461 ctggttcttc tccagatatg cctctatcga gccaatattg acatcgatct gtgcgatcac
     2521 attgttcatg ccgaccacac ccacagtata gccggcggaa catgccagaa ctgcaataca
     2581 aattgccacg cacaaaatca cactgcgata taatttccaa aaacgtttta ccatttttgt
     2641 aaaaacacac ctaccctttt atgaatccgt ttctctctgt tgaaagaagc ggatcgcctg
     2701 ttgtcatact caaggaaaca ctgcataaag cccgcctgac ggctttgcac gcaatctgct
     2761 tgcaga
//
LOCUS       DAWBGR010000247         2739 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8449_length_2739_cov_16.415797, whole genome shotgun sequence.
ACCESSION   DAWBGR010000247 DAWBGR010000000
VERSION     DAWBGR010000247.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8449_length_2739_cov_16.415797"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          654 a          810 c          542 g          733 t
ORIGIN      
        1 aagtgaactt ttcttgaaca actccaaccg ctaactgtcg atctcccgat ccgttccaca
       61 gtttccatta ttcccaagtt tcctcaactc tcgctctcgt gcgacagctt tattattata
      121 tcacacttct tccactgtgt caagtagaga attgtaaaga atacaagctt gattttattt
      181 attttgcaca tatgtattcc gtatacagca aaacaggctc tgacgacaca ccgccaaagc
      241 ctgttttaag cgattcatat tcttgttctc acttatccag tttctccgca atccgcttca
      301 aatacgcttc acaagctgct gcatgttgtt ttgtccggtt tgtgtcagat accatttctc
      361 cataagaaga aaccaacact tttatcagcc aaccatacat tgcaaacaga actgatgctc
      421 ctacgataat ccagccaatc ccgagttcgt cccaagacat ccagacaaat cctacaatgg
      481 ctccagctat cgcaatcagc accgatccta taaaatagtt ccttgccaat tttataattg
      541 tgtcgccgga acttcttgtt atttcgctgc cactgcttgt actgtttgag atatactgta
      601 cgcttttact ttcgcctgtt tgaaaatgat agccacaagt acaatctacc gcgtcatctg
      661 gatttgattt tccgcagttc atacacgttt tcacgacaaa ttcctccttt tttccagtgc
      721 gatctgcacc gttgtccaat atcagtatac cacagtattt caatattgtc aagcaatttt
      781 tatataatag tatttagaaa ttttactagg aggtattgtc atgtttgacg aacgcatcag
      841 agagctccgt cattcgctgg gcatcaacca agtggaattc ggcaaacgcc tgaatgtcac
      901 gaagcaatgc atcagcaact gggaaaacaa caacatcatg ccgtcgatcg acatgctgat
      961 tcggatttcc aagacgtttt cggtcagtgc cgattatttg ctcgggctgg aaaaccgaca
     1021 gacgctggac gtaagcggtc tgtctgccga acagatcctg cacctgcaag ccctcatcaa
     1081 cgatttgaaa cgcaactaaa aacgcccgcg aaacgcaatg gttccgcagg cgttttgcct
     1141 taggcaatac cgcacaaaga gcgtctggcg actttgcacg ccatctgctt gcaaatttcc
     1201 cgtcatattt cacaaaaatg ctcgtgaata catccagtat tcactccgct ttttgtttca
     1261 tctgacgaaa aatttgacga cgcatctgtc gcacaatctt tgtgcggtat tgcccttatt
     1321 tggtttccgg ctgttcttct gccgtttgct ccggcagaat ctccgccttg attcgcgaga
     1381 tccagttttc ttccatgttc agcaccgtaa accggatatt ttcccactgt accgacggat
     1441 tttcccgtgc ctccggaata taccccagca gatccaccac aaaaccgctc atggtgtcga
     1501 attccatatc ctccggcagc tcgtgcccca acagcggcag cgtttcctcc ggatcggcat
     1561 gtccgttgat gaggtacagc aaatccgtca cccgatgcac ctcggcttcc tcctcgtcgt
     1621 attcgtcctg aatattgccg acgatttcct ccagaatatc ctccatgcag accacacccg
     1681 cggtgccgcc gtattcgtcc acaacgatcg ccatttgtgc cttttggcgg gacatttccg
     1741 aaagcacgtc atcgcacttc gccgtttccg gtacaaaaag agcctcccgc atgaaatatc
     1801 tcacgccgta tccggaaata tcctgccgcc cgatcagccc cagcaggtct ttcgcgttca
     1861 gcactccaat gatattgtcg atattgtcct gatacaccgg catgcgggaa tatttctcgt
     1921 cctgtgccag cttcaccaca tcggcaatcg ccatttccac gtcaatggca accagttcct
     1981 tccggtgcgt catgacatca gaaatgggaa tatcgtcaaa ttcgaagatg ttgttgatca
     2041 tttcccgctg ctgctcctcg atcacaccgt tttcgttgcc ggcattgacc agatgcagaa
     2101 tatcttcctc tgtcacgcgt tcctttgccg gacgttttcc gccggcaagg cagtccatac
     2161 cctcatagat accgtagacc ggcagcgaca gcactgccac cagcttcacc aatcccagtg
     2221 actttcccgc caccagttcc gcctgctttc gccccagtcc tctcgcacag ccgtccagca
     2281 gcagtgccgc cgtcagcgaa accccagccg gcagcagcag tgtcagccag atgttccagt
     2341 gatgtgccca gccctttgca aaaatcagca ggtacgccgc cagtgtcagc agtgccgtga
     2401 acgagatcac ctccgccact gccgtgatcc gcagccggtt cttttgtttc agatagtact
     2461 gcatgggcgg gtgttcttcc gccagcgtgc tcatctgtcc gcccagcttc tgcaccgatg
     2521 ccattgccgc cgaaaaccac aggtgcagtc cgcacagcaa cgccgccgcc agaatcagca
     2581 cagagtccaa cgctgtgccg aacgtatcat agtccataat ccactcctaa aattatcctg
     2641 aatttatcat caggcagcac tgcacaaagc ttgtgtcatg ccgccttaag gcaataccgc
     2701 acaaagattg tgcgacagat gcgtcgtcaa atttttcgt
//
LOCUS       DAWBGR010000248         2739 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8450_length_2739_cov_4.640835, whole genome shotgun sequence.
ACCESSION   DAWBGR010000248 DAWBGR010000000
VERSION     DAWBGR010000248.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8450_length_2739_cov_4.640835"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          599 a          774 c          575 g          791 t
ORIGIN      
        1 aaaaatttga cgacgcatct gtcgcacaat ctttgtgcgg tattgccttt actttgcgta
       61 atcgaccacg cggctttcgc gaatcaggtt gaccttgacc tgacccggat aatccatttc
      121 gttttcgatc tgcttggcga tctgtcttgc caccagtacc attttctcat cgttgatgat
      181 ctccggtacc accatgacac gaatctcacg accagcctgc aatgcgtagc acttttctac
      241 gccctcataa gaagaacaga tgccttccag cttctgtaac cgcttgatat aattctcgta
      301 gttttcgctt ctcgccgccg gacgtgccgc agaaatggca tctgccgcct gtacgataca
      361 tgcgattgcg gttttcggtt ccacatcgtt gtggtgagcc tcgatcgcgt gaatgacctc
      421 gttgctctcg ttgtacttcc ggcaaacatc aacaccgatc tgtacatgag aaccctcgat
      481 ctccgcagtc agagccttac caatatcgtg gagcagtccg gcacggcgtg ccagattggc
      541 atcaacgccc agttccgaag ccagcacacc gcacagcttg gaaacctcga tagagtgcat
      601 cagcacattc tgccgatagc tggtgcggta atacagccgt cccagcagct tgaccaactc
      661 gttgttcagt ccgtgcacat tggtttccag cacggcacgt tcgccctcct gccggatccg
      721 atgttccact tcccgctttg ccttttccac catttcctcg atcttggtcg ggtggattct
      781 gccgtctgcg atcagctttt ccagagcaat ccttgccact tcccgacgaa catggtcgaa
      841 acaggacagg gcgattgcct ccggtgtgtc gtcaatgatg atgtccacgc cggtgagggt
      901 ttccagtgtc cggatatttc tgccctctct gccgatgata cggcctttca tttcgtcgtt
      961 gggcagtgcc acaacggaaa ctgccgtttc tgaaacctga tctgccgcac acttggcaat
     1021 ggcgagggag atatagctgc gagccttttc ctcacactcg tccttgatct gctgctcata
     1081 gtaggagatc ttcacagcct tttcgtgcac cagttcgctg tccagcgtgc tcaggatata
     1141 ttcctttgcc tgatcctgtg tcagtccgga aatgcgttcc agttctttca gctggctgtt
     1201 cttgacttct tccgcctctg ccagcttctt gtctgcattt ttcagacgct gcttgaggga
     1261 ttcttccttc cggtccagat tatctgtctt tttgtccaga ttttcctctt tctgctgaat
     1321 ccggtgttct tgtctgccga tttcagagcg tctgtctttg atttcttttt ccgcttcaga
     1381 acggagttta taaatttcgt cttttgcttc caccatggca ttcttccgct tggcctctgc
     1441 ctcttttttg gcatcttcca gaatgcgtcc ggcttctttt tcggcggagc cgatagtagc
     1501 ttctgcctgt tgtttccgct gttcaacgcc ggactgtacg cccttgcggt acgccagaaa
     1561 tccggcaatc gcggcaccca ccagcaaagc caccagacaa agcactgctg ccagtgccca
     1621 gttcatcgct tgcatgtcta tgcactacct cctttaaaat ctgttttgta tttgtctatc
     1681 tgtatacaac acagcggcgt tctgccaaag gcggcatgtg cgtcttgtat tcacttgtgt
     1741 ttcccgacag tcgggaattt tcctcaggaa gacgtgcatc tccttcccct ctccgtcctg
     1801 cacagcaaat ccgcggcatt ctcccttccg ccgaatcgct ttcagacagc atcacacaaa
     1861 gtcgccagac gctctttgta cgatattgcc ttatgcattg atgtcttagg acgtatcatg
     1921 aggatcatag acaatcccat ccaaaacgct gttcaggatc gcctttcttt cctgtatgcc
     1981 gtgcggcaga atcctgttcc gcacggctca caagtccatt tccagaaaaa aagcggtttc
     2041 ccacacatcg caggaactgc cttctctgct ctatcggaac ccattgccgg aacaaatctg
     2101 ccggcagacg ggatacatga ataaccacga cgcacacgca aaaaacgcag cgtcacccag
     2161 cattttcact gagaatcgtt tgtaatttga tacaacactt ttattttaca ctgtttccgt
     2221 gaaattgtca agacgtttgc agaaagattc acatttttct tgagaaacac tgaataaagc
     2281 ttgtgcgtaa gatgcgtgca gtgtttccct tacaagacac aaaaggaccc ggcacagcag
     2341 tgccgggtcg ggtttcaaaa tattatacag attctaatgc ttttttcgat ttcttttcgg
     2401 catgcttatg attttcttcg aactttttct tcacaaattt caaatcaacc tcttctatgt
     2461 aataagaggt tgcggctttt ccgatagaat atgtaccacc aaacgcaatc gtagcactaa
     2521 tagcagagcc agccccggga aatagtccat ttgctaattt tgatgtctgc tgagcaatca
     2581 acttaaaaag cattcctgca ccaaccactc cacctaagga cgctataaac tcttttgcag
     2641 catcaaacga caactctctt ccgcttaacg ctgcgatcaa catcaccata attgcctgca
     2701 aagatatcaa tatataagta tcagcaatag ggattggcg
//
LOCUS       DAWBGR010000249         2718 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8546_length_2718_cov_3.739392, whole genome shotgun sequence.
ACCESSION   DAWBGR010000249 DAWBGR010000000
VERSION     DAWBGR010000249.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8546_length_2718_cov_3.739392"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          689 a          649 c          548 g          832 t
ORIGIN      
        1 atttctatta taccatattg tctaacaaaa ggggagcatt tcatatagct ttgccggatt
       61 ggaatttcgc aaacgaaaaa aagttgcata aagccgtcag gtgggcttca tgcaacgttt
      121 ctttagtctt tgtaactcct atgttttcag atcacgcctc aaacggtgta atgataaatc
      181 ccaatttcat cggctgatct gcatggattc tgtactcctc atgcaccggt gcaccccagg
      241 aatcatcgcc gccgacaccc atttgctttg cagcaattct gacccatgtg tagcgaacac
      301 ttggaagttc atccagatgc attgcatttt cgagttcata ggcactgtat ggcagtacac
      361 tcatttcaaa cggagcagat gccttttgga acgtcagacc atgctgcata tcatcatgaa
      421 ttgagagcgt tcttacaccg gtacggttgc cgcattcctg cggattgaga tagccggaaa
      481 gattttcttt tgccgtagat ttccacaatc ccagtcttgc tcctttgcat cggtcgctgt
      541 agttttcatc aggaccgagt ccgtaatacc ggaaactgca aagctgtttt ttcatcttga
      601 agtccaatgc aagaaccggc atatcgggca ttccgcttac tcctgcataa tttacacata
      661 cgtccagctt tccgtcaaag tgtgcggtat aggttacagt aaactcaaaa gtcggaacac
      721 aggcagcctg atatgtgaat gtgattttca aagcatcttc ctgttccagc catgaaacgg
      781 ttttatactt tgcaaatttg cctgcggcat accactgtgc aagattgtac ggctcggaag
      841 caccggtgtc attgtctgtc atcgctctcc agaagctgac ctttggtgtg cgtgtgatat
      901 attcaaaatc attgtatttc agagaactga tgccgccctc ttttttgtcg aacatcatgg
      961 agaagttttc tccgtgaacg ccaatgataa catcgccgta tacaatcgtt ggcttttgca
     1021 atgtggtttc cggaatctga ttatctttta cttcaaacac ttcctgtgca aaggcaattt
     1081 cgtgccctgc atctgcccat agtgtctgat ctgcggtaac ggcggaaaca tgataaacat
     1141 attctcctgt actgtctatt ttgtgaagtg tcagtttcag tgtcttgctt tctccggcag
     1201 gaacattcag agaaaatgta tcggatttca gaacaacgcc gttcttttcc aatcgtacaa
     1261 caaaggacaa gttatttgta tctgcaaaga gatttcgatt ttctacggta agcattccgt
     1321 tttgaataga cattctgatg ttggagtaaa gagccttcat ttcctgaacc tttggagagt
     1381 aggttctgtc agcatagatc acaccattgg tgcagaatcc gtaatcgctt ggtctgtcct
     1441 caaaatctcc gccataagcc agaaccgttt tcccgtcgtc atttttggtt tcaattgcct
     1501 gatcgatgta gtcccagatg aaaccaccct gataagcctc atatttgtcc tcaaggtccg
     1561 tgtaaaggct caaaccgcca agagaattgc ccatagcgtg catatactca cagctgatat
     1621 acggtctgcc gttattctgt ttcaggtatt cttccacttc ctgcggtttt gcgtacatac
     1681 ggctttccat atccgtgata gaatcatact gatgattcgg aactcttgta acgccctcat
     1741 agtgcaccag tcttgtctgg tctgcacttc tgaaatattc cgacatcgca gcaatatctt
     1801 ctccgcagta ggattcgttt ccgcaagacc agatgagaac acttgcgtga ttcttgtcac
     1861 gttcatacat cgattttgct ctgtccagca cagcttcttt ccattccgga agtgaacccg
     1921 gtacattcca agacggatct gttgcaccaa gcttctgcca tgtaccgtgt gtttccagat
     1981 tggtttcatc aatcagataa atgccatatg catcacaaag ctgataccaa agagaattgt
     2041 tcggataatg acaggtacga acggcattga tattgttttt cttcatgaac tgaatgtcaa
     2101 acagcatatc ttcctttgtg attgctctgc ctgttttcgc atcaaattcg tgtcgattga
     2161 tacccttgaa aataatacgt tttccattca ggcacatgat tccgtttttc agttcaaatc
     2221 ggcggaatcc aaccttggtt ctgctgacct cgatttcatt tccgctttcg tctgtcagaa
     2281 tcactgtaaa cgtgtagaga ttcggtattt cggcactcca aggcttgcta ttttcaattg
     2341 tccagtttac agattccttt tcattggatt ctgcaatgac cgttccattt tcatctgtca
     2401 agatcgattt cacagaacac ttgcccgcaa tatccagcgt tgcagagaag ataccatttg
     2461 taccgtcata atctgcaatc actttcatat cacgaacatg ggcagacggt gccgcataaa
     2521 ggaatacgtc acggaaaatg ccggagaatc tccagaaatc ctgatcttcc agccagcttg
     2581 cagaacttct cttgaacact gcaacagcaa gtttgttgtc cttttcacgg atatatggtg
     2641 tcagttcaaa ttcagacggt gtaaaagaat cctcgctgta gccgataaat tcgccgttca
     2701 gccagacata catagcag
//
LOCUS       DAWBGR010000250         2695 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8640_length_2695_cov_3.124242, whole genome shotgun sequence.
ACCESSION   DAWBGR010000250 DAWBGR010000000
VERSION     DAWBGR010000250.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8640_length_2695_cov_3.124242"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1952..1961
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          784 a          562 c          604 g          735 t
ORIGIN      
        1 tctgacgaaa aatttgacga cgcatctgcc gcacaatctt tgtgcggtat tgcctttaaa
       61 atgtatttgc gggggatttc tgaaaaacaa gcggggacag ggtacgaaac cgattttgat
      121 gcatttggat gattcaggaa gcacggaata aagccagcct gacggctttg cacgcaatct
      181 gccgaaaaat ctgatgacgc attttacgca caagctttat gcagtgtttt cttcactttt
      241 tgcggaggca acatcgtgca aaactagcag atggtttaca tatgaacttg tttaaaattg
      301 gaaaatataa ttattatcat atcgactatc gaacataata ttcatgttat gattataaca
      361 taattctctg caattgtcaa tagaaaatct ctaaaatgcg tttaaaaagt tcgagtggac
      421 ataaaagtag cgacattttc tccaagtcag agcaaatgtc gctaaaaatg atttgtttca
      481 aaattttata tcggcacata ttgaaagtca gtatgccttc ggaatgtcaa acatcttaca
      541 attccacatg atgtctgcca gatccggcgt ttttccagtc ttcatgcact cgtacaagta
      601 ccggaggatt ttgtacatga taacttccat gtcgtttttt gccattgtgt gctttatcta
      661 taatccgttt aatctatagc ttcaattgtt ttaatttcgt ctgcgtcaat ccaaacgcct
      721 gtgtccaatg caattccgtc tacgccgtca ttgtcaggtg ctgggacaaa cacgttacaa
      781 taccctgaaa tcattgttcc atcatctttg taaatgatgc gaacagtgtg cttataatat
      841 tgatttaaac tcatttcaca tcatcgcttc cttttcttgg gtacaacgtg cgtccttttt
      901 tcatctcaaa ttgcccttgt caatcaaaaa cagttccgaa aatgtcccgt atttttggaa
      961 ctgttttgta tttactctcc tgaaaaactg tgctatgctg tttgcaggag gtgagaacag
     1021 atgtttatga atggtgagat cttaaccgat ttgaatgact taaaacgctg tttttccatt
     1081 gatgaactgc tgtacagcta cggcaatggc gaactggaaa tttttcttga aaagatcggc
     1141 gagcatgaaa aagcagaaca aattcaggaa atttccgaaa acaacgccct gctgctgatt
     1201 cggctgtatg atattttgga tttgccgtat gaagactcgg aggagaagat ccggcgtaat
     1261 tttgcgtaaa gccggaaatc ttttgtggtc tgaccggcac accccaagag cacaccgctc
     1321 ctcgttggcg tgctcttttg tgctacaggg agattgccac acagttcctc atatccaaag
     1381 gaaacattgc ataaagcacg cctgacggat tgacacgcaa tttttgtact tgccatagta
     1441 cttcaaaaag tgtataatga aggaaacacc gcacaaagcc tgcccgacgg tttttacgca
     1501 atctgccaac gcaccttacg cacaatcttt gtgcggtgtt gccttaagag agggggagaa
     1561 acatgcatgg gctgatgcca aatccactga caaaattatc ggaaagtact gaagaagacg
     1621 aagtccttcg cagcagaaaa cgaaaactgg acatggaagc agaatcagac ggcgaacagc
     1681 tgcgggaaca gcttcgtctg catctggagg acaagcggat tctgaacggc gatgccggaa
     1741 aacggctcag gcgggatttc ggaagacttt tgaaaagcgg cgaggaggga gtgccaatgg
     1801 cagatataga ttacgagatc tacaagtata tcaacgaact tgccgatcag tacatagaac
     1861 tggacgatct gctggaacag aacggcgttt cctacagcga agcactggag caggcagcac
     1921 aatttcggaa acagtttttg tgcctgctgg annnnnnnnn naatcaggat ctagttgtca
     1981 cttgctggga cttaatggaa cgtatcgagc agcagccgga acatccggat ttgcagtacc
     2041 tctatttctg tgttgtaaca gactttggtc tgttgaatgc agtcaacgcc gcagaccgca
     2101 cggaggaaca gaagatccgc aattatttcc gccagcagaa cgcactgcgg gaattgacag
     2161 acttttttca ggcacagtct gaaaatttac agaaatatca tgcattgctg gaatatctga
     2221 aaaagccgcg gcgtgtggaa agcggtgtcc ggaacgagga agattttgac ttgctgtatc
     2281 atctcactgc acagcacgcg tttttgtacg gctgcaaaac caatccggtg tacgaggaaa
     2341 atctgcgtgc cctgcaactg cacatcaaca acgatgtcaa gttaaaagcg gtcaagccgt
     2401 atatcatttt cgcggtgctg gcaagaaaga ccggtatgat gcagaaacga aagtacttca
     2461 tgccgaatct gaaatcgatt tttcaatatc agaattataa tatctacaag gacaacggca
     2521 aaaatttcaa ccagtatgct tccgagctgg aactctatga ccatttgaga agaagctaca
     2581 tcgatgactg cgaaactgac gtgggattgt gcgatttttg ctttgcgaat ctttctccgc
     2641 ttagtgagtg gtattatcag aactgtgaac cgaatgagga gattcccatg gctct
//
LOCUS       DAWBGR010000251         2678 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8697_length_2678_cov_4.958445, whole genome shotgun sequence.
ACCESSION   DAWBGR010000251 DAWBGR010000000
VERSION     DAWBGR010000251.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8697_length_2678_cov_4.958445"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          544 a          714 c          843 g          577 t
ORIGIN      
        1 acgcactgcc agctcagcac aaggcacgcc cttcgggtgc cctttcaggg gagcctgaat
       61 aaaagcctcc cctgaaaggg gagggggacc gccgttaggc ggtggagggg ttcttttgtg
      121 aagtcttttc ttggtgtaaa agagttacga gataacccct cagtcaagcc tgcggcttgc
      181 cagctcagca caaggcacgc ccttcgggtg ccctaaagtg gagcctaatt attattttag
      241 agtttacttc tgcggcttct gcttcttgtg ttcggggcgt tttttcttct ttttccggac
      301 ggaatccccg aagttcccca gctttctgcc cagagcgaag tattcgccgt gggcgtatgc
      361 catcagaccg cactgctgga gattcagctt gcccttgctg tcgaggtact gttcctcgac
      421 gttgacaccg gtgatctccg cttcgaatac cgtatgggtg cccagttcct tggtggcggt
      481 gacgcggcat tccagactga cggggcactc tgcgatcagg ggagccttaa cggtctgtgc
      541 cggctccggt gtcaggtggc actgggcgaa cttgtccacg tccctgccgc tgcggacacc
      601 gcagaagtcg gtggctttca tcatggcaga ggtggggaga ttgatgacga attcgccgga
      661 tccccgaatc agatcatagg aatagcggga gggacgaacg gaaatatatg tcatgggcgg
      721 gtgcgttgcc agcacgcctg tccatgcaat ggtcagcacg ttggggtgtt ccatggtgcc
      781 gcaggtgacc agcgtgggcg gaacgggtgc cagcaggacg ctgcctttcc agtgttgttt
      841 tgccataagt atctgctcct tgctttcggc agtaccgtgt gcggtgtcgc ctttataata
      901 taggctattg tatcacggag cgggcggtgt gtcaagtgcc ggcgggaata tttgggcaat
      961 accgcacaaa gagcgtctgg cgactttgca gacactcctc gttgacatct tctgcaaaac
     1021 cttgtataat acaggtagca ttcgaacagc accgtctgat gccgtgctgt ttgaaaaaac
     1081 aacgaggtgg aattcatatg atacattgtg cagccatttt tacagaccac atgctgctcc
     1141 agcggggcaa gcccattgcc gttttcggga cgggcaccgc aggggagccg gtgactgttt
     1201 ccgttcccca gcggcactgt accgtttctt ctgcggtgcg gcaggacgga acatggcggg
     1261 tgacgctgcc gccgatgcct gcgggcacgg gctgtaccct gacggtaaac gaacaggaat
     1321 tttccgacgt ggcattcggg gaagtctggc ttgccggcgg gcagtccaac atggaattta
     1381 tgctgaaaga tgccaggaac ggaacggcgg aactggagca gtgccggaac tccaacgtgc
     1441 ggtattttca ggtgccgcgg aatacgttcg cagatgatgc ctacactgcc gcgtgggacg
     1501 ctgcccgctg ggaactgccg gatccggaaa ccagcggcac atggtctgcg gcggcgtatc
     1561 tggcggcgaa ggaactggca cagacacagg gcgttcctgt ggggattatc ggctgcaact
     1621 acggcggctc atcggtcagc tgctggctgc cggaatccga tctgcgggca cacactgccg
     1681 gtcaccccta tttgcaggac tacgccgatg ccgccgcggg aaagacggac gcggaaatga
     1741 tcgccgagta tgacgaatac gtggcatatc acaccgcatg ggagagccgc atgcagaagt
     1801 gctatcagga ggacagcaat atgccgtggg acgagatcat tcgccgctgc ggagaaaacc
     1861 gttaccccgg gccgctgggc atcaagagcc cctaccgtcc tgccggcatg tatcacacca
     1921 tgctgcaaag ggtggtgcct tataccattc ggggattttt ctattatcag ggcgaaaacg
     1981 acgagatccg accggatact tatggtacac tgctgacaat gctgatcgcc cgctggcggc
     2041 aggattttga agatgtacag ctgccgttca ttctggtaca gctgcctatg cacacctatg
     2101 cggacacgcc ggaaaacggg gcgtggagcc ggctgcggga ggcacagatg caggtatacc
     2161 agacggtaaa gcacaccggc attgccgtga ttctggactg cggcgaatac tgcaacattc
     2221 accctgtgga caagcaccct gtgggacacc ggctggcgtt gcaggctcgg cagctggtct
     2281 acggagaacg ggagctggag gcgttcgggc cgatcttccg caactttgtt gtggagggca
     2341 gtacgctgac ggtgttcttt tcccatgcgg cacagggcat ggagtggcac ggggaaccct
     2401 gcggcttcca gatcgccggt gcagacgggg tgtatcacga tgccagagca gtgctggacg
     2461 gcgacacggt gcggctgacg gcggcagaag tgccggctcc tgctgcggca cggtacaact
     2521 gggtgaacta cggtgcagtt acgctgttcg ggaaaaacgg gattccggcg gcaccgttcc
     2581 ggacggaagc ttctccgctg ccgctgacgg cagtttgtga ataaaggcaa taccgctttt
     2641 tgtttcatct gacgaaaaat ttgacgacgc atctgtcg
//
LOCUS       DAWBGR010000252         2671 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8728_length_2671_cov_5.487003, whole genome shotgun sequence.
ACCESSION   DAWBGR010000252 DAWBGR010000000
VERSION     DAWBGR010000252.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8728_length_2671_cov_5.487003"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          548 a          659 c          782 g          682 t
ORIGIN      
        1 tgcaagcaga tggcgtgcaa agtcgccaga cgctctttgt gcggtattgc ctttaataag
       61 cggcggttgt agtgccggtt tcagatgccg gttctgtaga accgctgtcc ggcgtgacag
      121 ctccgtttgt cagggtgatg ataaagccgc tgctgttgtc accggaaacg gtgtagactg
      181 cattttccgg cagggaaacc gttgtggtgc cgctggcatc tgcgggaacg tagtagatcg
      241 tgtaagactg tacgccgtca gaatatgcca gcccgccgga ttccatggtg taattccgga
      301 gatagtccag gtattcctcc agacagaagt tgttttcgtg catgatcgcc gcgtgaatct
      361 tgcccacata ccggaagtgg tgcggggcgt agggtgtgcc ggtggtttct gtcttgtcgc
      421 tgggataccg cagaatgaag ccgtattccc acgagttcgt caccatccac tcattgcgtt
      481 tggtgatgta gggaacaact tcgccggtgc tggtgatcag accaatgtca aagccgtagc
      541 cgctgctgcg gtcagggaga acattggtat aaatggaggt gctgctcagc gtggtgtcca
      601 gcgtgctgta aacctgtaga ttggcatagc catttgcagc ggcgtatgca tcgaacagac
      661 tgcacagcgg ctccgtgatt tccgactgca tttgcaggga agtatctctg ggcttgacgt
      721 tggtgtccgt cacggcggaa aagtctgtcc atgcctgtgc accggcatag ggatgtgcgg
      781 catctaccag aatcagagag ccgtttgcct catcggctgc ggtcagctgt agtactgttc
      841 cgctgggttg tgttgcttcg gtggtatcag cgttttcctc tcctgcggaa gaagtgtcag
      901 aggcggacgg cgtagaagca aatccgccca cgcccacaat attttcggga acattctgca
      961 agaaaacgcg gtaaaatgcg tccagtgcga agatcccgac aatcaggagc actaacgcaa
     1021 ggagaaagcc ggcgggactc atgtgtcttt gtttccgttt gctcatagcg tggaaccagc
     1081 ctcctttcct gtgaaagctg ctgtttcggt tatgcagccg gcacagcagt atcagtagta
     1141 tcggtgaacg tgtctgtagg gtccacggca gaaccgtttt catcggtttc cactgggctt
     1201 aggtctgtcg gcataggata gccgttgtgg atcagaccat agatcgcaaa ggtatcgaat
     1261 acggctctcc aataagtagg gcctttggcg gtgacagcga taaaatgctt gccctcggtg
     1321 tcagtggcat agctgaccag acagttgcct gcctcgtcgg tgtagccggt ctttccggca
     1381 tcaatggaaa tgccggtgac ttcgttgccg tacatgcggc tgtacatggt gctctccagc
     1441 agaatgcctt ccgggtgctg ctctgtggca gtggtggtgt actgataagt gcccagaatt
     1501 tcgcggcagg tatcattttc catgaccgca gacatcagca tggcaatgtc tgtggcagtg
     1561 ctgtagtggt tggggtcgta aagtccgctg gcgttggtga agtgggtatt tttcagcccc
     1621 agctcttcca ccttttcgtt catcagcttg acgaattcgt cctcagagcc gcagagataa
     1681 atggcgatcg cggtggtagc atctgcacca gagggcaatg cgatcccgta catcagatct
     1741 ttcagggaaa cggtttcacc ctcttcaaag ccggcacggg aagcttccgc ttcgatcagc
     1801 ggggcaatga tctcgtaagt catggtaaat gtatcgtcca tgttttcgat atgttccgcc
     1861 gccactaaaa gcgtcatgat ctttgtcatc gatgccggat aaatgcgtgc gtccggatcc
     1921 cgctgtgcca gaacggtgtt gttttccacg tccagcagca cagcatgttc gctggagata
     1981 ttcgaagcgg tcagttctgt gaggttgtct gccggtgtgg gatatgtcag cagggcaggc
     2041 ggttcggtgg gagcctctgt gggggcttcc gtctgtgtag caaccggagc ctgtgcattt
     2101 gccgccggag aaagctgctg ctccaccttg ggcttgccgg tctgtgtcag aagtacgatg
     2161 ataaatacta ccacgataaa tccgcaaagg acaccgatca tggtattaat ccagagtttt
     2221 gtattgtggg gatttttctt tctgcgaact ttcattgctg cacctcatac caaaattcga
     2281 tcttttcagg gcgtgtcctt gtccaaacac ggaaacacgt ccgcagattt tgtgcggcag
     2341 ttctacggca aggaagcaga aaacctgttt tttgtcggac tgcctttaca attataatcc
     2401 atttttaccg ttttgtcaat ggatttagag gattcttcat ggaaatctgc aacatcgccc
     2461 cgcaggcttg tgcataagcc tcccctgaaa ggggtgactc cccacggggt ggggagatgc
     2521 ccgcagggca gaggggaccg ctcagtacga gggggaccgc cgttaggcgg tggaggggtt
     2581 cctccgcaaa gtgtaacttc tagtgctgcc atgtcaaaac cgtccggcgg aattcgctcg
     2641 ttgccctcgc tgtccgcctg gggcttgcca a
//
LOCUS       DAWBGR010000253         2659 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8779_length_2659_cov_4.898618, whole genome shotgun sequence.
ACCESSION   DAWBGR010000253 DAWBGR010000000
VERSION     DAWBGR010000253.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8779_length_2659_cov_4.898618"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          585 a          680 c          676 g          718 t
ORIGIN      
        1 aatttgcaag cagatggcgt gcaaagtcgt cagacgctct ttgtgcggta ttgccttcat
       61 tatagcatgt tttttggggg attgcaaggg ggaatgggga actttgcggg gacaccgttg
      121 ataattgagg ggttatctca taagcctgtt acatgaagaa aaagattcca cgaagaaccc
      181 ctccaccgcc tgccggcggt ttccctcgta ctgagcggtc ccctctgccc tgcgggcatc
      241 tccccacccc gtggggagtc acccctaaag ggaaggcttt ctcgttcagg ctccctcttt
      301 tatttcaggc tcccctttag gggagctgtc accgcaggtg actgaggggt acttccgtgg
      361 aatctgatac aacagggaaa agatttacga aatatccctc caccagcttc gctggtcccc
      421 ctcccttttc aagggaggct ttttcattca ggctcccttt ttgtgtatag gctcccctat
      481 agggcacccg cagggcgtgc cttgtgctga gctggcaagc cgtaggcttg actgaggggt
      541 tatctcgtaa atcttttaca tcaagcaaaa gattccacga agaacccctc caccgcctaa
      601 cggcggtccc cctcgtactg agcggtcccc tctgccctgc gggcatctcc ccaccccgtg
      661 gggagtcacc cctaaagggg aggctttttc aggctttccc gcctattact cattataaat
      721 aaacgactga tacgcgtcgc cgttttcgct ttggggcgtg ctgggtttgc tgccggtctt
      781 tttgctgctg ggcggggtat ctttcgtgtc cacgtcgaca cgggtgcggt atccggcttc
      841 cgtccagcgg gtgaggatcg agttcagata ttcgaaaacc agtttgcccg tgctctccac
      901 cgtcttttcg taagcgtaga cgatcagttc catggggatc tttttatcct gccacatgtc
      961 gatgaactcc tgctgtttcg tggtgggctt ccggtgcatc tcgaaggctt tctgaatgtg
     1021 tccggtaaag ctgcggcggt attccatatc gttaatggca tcgttgacct gtgtcagcgt
     1081 gtgaatgccc tcgttccacc actgcgtaat gatgctttcg gcgtagttga cgctgcactt
     1141 gtcgatggac ttgcagtaga gcagcaccgt gagaatgatc tcgctgccga tgttgttgcc
     1201 gtcgtgcatc cagacgaggg acttctgctc gatgtgccgc agcggtctgc ccagctgctg
     1261 ctccgacatg cgaaacaggt ctttcaagtc ctcggaagct tccagtgcgg tggaaatttc
     1321 cagcgggctc atctcgccgc attttttgct ggtctggagc agatgcggct gactgccgcg
     1381 ggactgcttt tccgcagccg gtctggctgt gggctgctcc gcgttttttt gcggctgttc
     1441 ggcagactgt tcttccacag tgaacagggt agtctgcgac cagaacagca ccgcttcttc
     1501 caccagttcc tccggaatgc ccagagcctt ggcgatctca tcgtgtgcaa ggttcgggcg
     1561 gttgctgcgg agcagataga gcagcacttt cagctgggac ggcgttgcca gcttcagata
     1621 ttggtctacc acggcattcg gcacgccgaa catctgcccg aagccggaga tatgtacgct
     1681 aagattcaca aaaaattcct ccctgcgaaa aaattgcaaa aaagacttga cagcggttga
     1741 cgtttgtgct ataattagtt atgttgttgc cgacatgttg gaattggcag acgagacgga
     1801 ctcaaaatcc gttgccagca atggcgtgtg ggttcaagtc ccactgtcgg caccaaattt
     1861 gatcgcctgt accttttggt atgggcgatt tttgttttcc atgggagatg ccccatgaaa
     1921 ttctatgcct gtccggcggg gaacatgccc gactgccggc aaacagatag aaaaaaaacc
     1981 catgctacat aatgtaacac aggtatgttt ccgtacttcg aaagaaatac ttggcgtgcc
     2041 cagagggatt cgaacccccg acctactggt tcgtagccag tcactctatc cagctgagct
     2101 atgggcacat tttcttttga gaaccgttgt cctcacaaca ataactatta tatcacatct
     2161 tttgcagctt gtcaagcgtt ttttcgaatt tttttctgga ttttcgaaaa aagtctgtat
     2221 cgcacgcaat ttcggtgttt tttgtttagg agagtgacag aaagggcaaa cgcagtgcat
     2281 ttgctgccaa gcaaaaacag acttgacaaa ccggagaaaa tcgcttataa taaaagtaaa
     2341 gagagcacac cagtagacgg tctgctccca tttacgaata tgttatagaa taatcgtcac
     2401 aagttgggga acttcagggg cggttatttc tttttcttct tgtgattacg gttgccatgt
     2461 tttttataca atgaagtgat aaatgttgcc agagaaatca gaaacgttcc gatttccagc
     2521 aaatcacgaa atgtaacaaa actatccact gcaatcacct cccatatgct ggattatgca
     2581 aatgggaggg agtactccga acaaaccgcc taccgtttca ggtatgccct cggtttttta
     2641 ttatatcaca ctacgcgaa
//
LOCUS       DAWBGR010000254         2630 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_8891_length_2630_cov_5.158835, whole genome shotgun sequence.
ACCESSION   DAWBGR010000254 DAWBGR010000000
VERSION     DAWBGR010000254.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8891_length_2630_cov_5.158835"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    713..722
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          623 a          728 c          697 g          572 t
ORIGIN      
        1 taatcttcgg gtgatattgg aatcttacag tacaggtgga acgcgaactc ttttcttttt
       61 ttgcttcttt ttttcttttc agtaaagaaa aaaagaagcg taactccttg tgaaaccggt
      121 tggctactgt agcggggaac ccctcagtca agcctacggc ttgccagctc agcacaaggt
      181 acgcccttcg ggtgccctaa aggagagcct tattcacaag cttgcaaacg ccatatcaaa
      241 taaagttgac cgtttacaca agtaacgcct tagaaaaaca acactgcaaa tacaggcaag
      301 ggcaacaacc ggcgttggcg agcctcaggc gaacagcgag gtacgagcga attccgccgg
      361 acggtttcga catggtaaag ctacacttca cgagggaacc cctacacatt ccaagaggtg
      421 catctaagga aacactccag tcaccccttt ctggaaaggc ttatgcaatc acccgcaaaa
      481 acataaggca ataccgcaca aagagcgtct ggcgactttg tgcggtattg ccataaaaaa
      541 agagcaaacc acatttcgtg atttgctctt ttctgcgttg gtgacccgta cgggaattga
      601 acccatgatt ccgccgtgaa agggcggcgt cttaacctct tgaccaacgg gccatatggt
      661 agcggcagca ggattcgaac cagcgaccaa tcgggtatga accgagtact ctnnnnnnnn
      721 nnagtactct agccaactga gctatgccgc cacatcgaaa caggtttcgt tcagtgcttt
      781 ttgtttactc ctgaattcct gcgactatga tagtatacta cattttcgga taaatgtcaa
      841 gcactttttt gaaaaacggc aatttataca actgtacaac aaaatcaaaa ttaattctac
      901 agcatgttgc ataaattccg aggctgtttc gctgaaaaaa gacctatttt tgcttcgggc
      961 gaaaaattgc ggcacagacg acaccgaaca ccattgccgc agtgatgccg gcggaagatg
     1021 cagtcagacc gcccatgaag atgcccagca gtcccttttc gtccacggat tcccgcacgc
     1081 cctctgccaa caggttcccg aagcccgtca gcggcacgct ggcaccgccg cctgccagtt
     1141 ccaccagttt cgggtagatg cctactgccg acagtgccac gccggcaacc acatacgccg
     1201 tgaggattcg tgccggtgtc agcttggtgt agtcgatcag cacctgcccc acggcacaga
     1261 gtgcaccgcc aaccagaaat gcccagaata gatccattgc cattgttgtt cctcctattc
     1321 atcgccgcag aagtgtcagg gcaacaccgc acaaagcttg tgcgtaagat gcgtagtcag
     1381 atttttcggc agattgctta gaaattccgc aggcatactt tcgtatgaca agggatttct
     1441 gggcaatatg gcggaaaatc tgtacgcaga ttgcgtgcaa agccgtcagg cgggctttgt
     1501 gcagtgttgc cttaattcta ccaaatgcga aatacccgga atgctctcgc cctgctgaga
     1561 agcagtaggg gacatcaggg caccggttgc cgcaaacagg atctgcttca tggctccggt
     1621 ttgcagagcc ggcagaaagt gcccgcacag catgctggca ctgcacccgc agccagagcc
     1681 gccggcgtga gtgtcctgcg ttttccggtc gtacagcatt gcaccgcagt cccgatgcac
     1741 cgcagaaata tcatagccct gcttcatcac cagatcgcag agcagctcgc tgccgatcag
     1801 ccccagatcg ccggtgacaa tggcgtcata cgtgtccggt gctgtgttgg tgcctttgaa
     1861 gtaccggaca agggtatctg ctgccgcagg agccattgct gctcccatgt ggttggcatc
     1921 tgtcacgccc agatctacca tggtgccaat acagccgccg gtgacacaca cgccgtctgg
     1981 tatgccgtcg ggctgctgta ggatcacggc tccggagccg gtgcatgtcc actgtgctgt
     2041 aggcggacgc tgcccgccgt aggacagggg aaaccggaac tgacgttctg cggtggagaa
     2101 gtgggaagat gtcacggcgg cagtgtactc gcctacgccg ccggctgcca gaaacgatgc
     2161 cagcagcagc ccctccgcca tggtggagca ggccccgtaa atgcccagca ggggaatctc
     2221 cagcggacgc atgccgtagg tagagccgat gcactggttc atcaggtcgc cggcacagat
     2281 gcaggagagg tcgctgtcct gtaagccgca cttatccagt gccagctgga cggttcgccg
     2341 ctgcatcacg ctttccgcct gttcccatgt gtcctgtccg aaatgagcgt ccggctccac
     2401 gcagtcgaag caggcaccga aaggaccgtc gccctctttt tgtccgacgg tgctggcgta
     2461 gctgatgatg cggggcggct gggcgtattg gaatacgccg cgggataaat tgcgtaccat
     2521 tcgaatcacc tcgcggatag tatgtgttgg tttcggggaa ttatgcaagg cataggcggc
     2581 gggtcgccgc tgaccttacg ccccaatttc aactgtgaaa agggtaatgt
//
LOCUS       DAWBGR010000255         2596 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_9042_length_2596_cov_5.080677, whole genome shotgun sequence.
ACCESSION   DAWBGR010000255 DAWBGR010000000
VERSION     DAWBGR010000255.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9042_length_2596_cov_5.080677"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2312..2321
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          662 a          623 c          732 g          569 t
ORIGIN      
        1 tgacgaaaaa tttgacgacg catctgtcgc acaatctttg tgcggtattg cccagtattc
       61 tcgggctgtt gttttttggt gttatgcaca ggcagtttcc agtgcgattc gcatcatatc
      121 ggtgaaagag gtctgtcgtt cttctgcggt ggtggattcg ccccgtagcg gacagtccga
      181 aacagttacg atgcacaggg cttcctttcc ggcggcagca gcattcatgt acagtgcagc
      241 cgactccatt tccgtggcaa gcacgcccat tttcgcccac tgcggcaaag ggcttggctc
      301 gttgtaaaac acatcggtgg agagcaggtt gcccacgtgc accggaatcc ccagcctgcc
      361 ggcagtctgg taggcttgtt ccagcagggc gaaggaggcg ataggggcgt atgtgccggc
      421 gaggtgatac tgtgccgcat agttggagtc cgtgcaggca gcctgtccca gtaccacatc
      481 acggaggtga atgtggtcgg caacgccgcc ggcagtgccg atgcggagaa tccggtctac
      541 gtcatactcg tggaacaact cataggaata gatgccgatc gagggcatgc ccatgccgct
      601 gccctgtacg gaaaccggta cgccgtgata ggtgccggtg tagccgtaca tgccgcggac
      661 ggtattgtag cataccgcat tttccagaaa atgttctgcg atgaatttcg cccgtagcgg
      721 gtcgccgggc atgagtacgg ttttggcaat gtcgcctttg gcggctgcgt tatgaggggt
      781 tgccataaaa atcagtcctt tcacatatga aatgaggtta ttttctgcgg cgaagctgtc
      841 ccagtgtctg ggtgatagcg ttgaagttgt acagcaccat aaagacaaat cccacagcca
      901 gaatcaaaat ccacataggc ggagcctcca ccacgacaat gcccagcagg aacagggcgg
      961 cggtgttggc ggcgaatttg gcgtggttga ccggaaacgg aacatacttc cgtgcgtcca
     1021 cgatccggat acagtagcag acaaagtagc tggcgaatgt ggcaattgcc gcacccatga
     1081 tgccccagat gtgaatgagg ataatgttca gcacgatgtt cacgattgca gccaccagac
     1141 tggtccagaa cgagtgtgtg gtgtgctggg tcgcagagta aacgctgctc aggaactggt
     1201 tgaagcacat catcagcacg cctacgatca gtaccggcgt atagaggaac gcggtggcgt
     1261 attccggata ggtgttggaa tcgatcagaa tgttggacag gatccggaca aatacgatca
     1321 gtccggtact ggcgataaac atgaacgact ggtaggtatc gaatacacgg tgatagaacc
     1381 ggcttctgtc cttggagtcg ttttcctcaa tggcggacat gttccacgcc tgaaagaaaa
     1441 tcgtggaaat ggtggagatc aggttcggga tcttggtgga aacaccgtag ataccggcgg
     1501 cagtttcgcc caccagtcct gccggaccgg acatgtaccg gataaacagc cggtcggaaa
     1561 agccggtcac cagccacagc agggcggacg ggatcagcgg aatggaaaac cgcagcatca
     1621 tgcgtaagat cttcttgtcc atgtaccgca gccggaagta ccggtgcagt ccggcgaccg
     1681 cccacaggaa cagccccgac agcaggtcgg acaagatcac ggagatcagg aagcctgtta
     1741 cacctagatg gaatcccgca ataaaggtca cattgaacag gaacagggtc agcgtggcga
     1801 gtatgccgtc tatggcaaac aatcgcacca accctcgtgc ccgcacaaac tgggaggcga
     1861 tctgtcggaa tccggaagtg atacagtaga tcaccagcag caccacatag ccctctgtat
     1921 agggcagcag ggtgagtgcc ggcgaaagca gcagcagaat gccgatgccg aacagctctg
     1981 tcacacaggc ggaagtaaag accttgcggt tgtcgaagtt tttgtcaatg ccgtagcgaa
     2041 tgactgcatc ggcaatggaa aaagtaatga tcggaatcag aaagtttgcg gtctgttcca
     2101 gcagctcctt cgtgctgttg tcgccggggt tgatgtttcc cgtataaagc cgcgtcagca
     2161 gcaggagcag aatcttggaa ccgaatgagc cgatcgcaaa tatcagcgta ttcgaaacga
     2221 gttttttgta tttgttcatg aaagcccacc tttatctgtg tttcaccgca ccggaaaatc
     2281 tgacaacgcc tctgatgcac aggtttcgca cnnnnnnnnn nttataacat aaatcgatgc
     2341 aaaatggaat ctttttttgc aaatctctgc cggaaaacag aatttgtgca tctttaacaa
     2401 tttgatgatc gcaaaattgt acatatatac aaacctcaaa aattttttct ggaaagtgca
     2461 acatttcggg gtgctcgatt gtgtatatag tgaagggatt ttttgtgggg tgaagaaccc
     2521 cgcagaaatg ggcgatcaga aatcgtccga aaggaacgga aatgtgaaat cataatggaa
     2581 acacagaata aagctt
//
LOCUS       DAWBGR010000256         2582 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_9116_length_2582_cov_4.072418, whole genome shotgun sequence.
ACCESSION   DAWBGR010000256 DAWBGR010000000
VERSION     DAWBGR010000256.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9116_length_2582_cov_4.072418"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          576 a          733 c          602 g          671 t
ORIGIN      
        1 ctgccaccag atttgccggc agctgttcat agcgggcaaa tgccatggag aaggttccct
       61 cgccctgtgt catctgccgc agctgcaatg cataatctgc catttcccga agcggtactt
      121 ctgccgaaat gaaagtcata ccctgatctt ccacagaatt catgcccagc actctgccgc
      181 gacgcttgtt caggtcgccc ataatatcgc cggtgcattc gtccggaacg gttacgctca
      241 tcagtcccac cggttccagc agcgtcggtt ctgcctgctt catgccttct ttatatgcaa
      301 gagaagccgc cgtcttgaac gacatctccg aagaatccac agggtgatag gaaccgtcca
      361 ccagaattgc ctttacgccg accaccggaa agcctgccag aacacccttg gtgtaggatt
      421 cctgcaagcc cttttccact gcggggaaga aattccgcgg cacagcaccg ccgaatacct
      481 tttcttcaaa gaccagttct tccgacggat acggttcgaa ctcgatccag acatcgccgt
      541 actgtccgtg gccgccggac tgtttcttgt acttaccctg aactttgacc tttttccgaa
      601 tggtttcccg ataagcgatc ttcggcacgg aaagtaccac atctacccca aaatcagctt
      661 tcagcttgga aatcaccgca ctcaggtgct gctcgcccag tccggagagg atcatctcat
      721 tggttgccgg attctgctga taggacaagg tctggtcttc ctccagaatc ttctggatcg
      781 ccgcagccac ctttgcctca tcgccccgtg ccgccggacg cactgccata ctgtagcacg
      841 gtgtcgggaa gctgctgcgg ggcaggctga ctacgcggga actgtcacac agtgtatcac
      901 cggtattggc ggaaagcttt gtcactgcca ccagatcccc tgcttctgca acagccgttt
      961 ccgtctgctt cttgccgcag aggaagaaca gtttgttcag tttttctgtg gctccggttt
     1021 tgctgttcac cagttcggca ccagctttca gcgtgccgga aatcacccgc atcatagaca
     1081 gtttgcccac ataggggtct gccagtgtct ggtatacctg cacgcagacc ggttcttccg
     1141 gcgactggtg cagtacgact tcttcgccgt tctggttcag tgccgcgatc tcgtcagaag
     1201 cttccggcac caacagttcc atttccttgc acagcaggtc tacgccctcc agcatctgag
     1261 ccgaaacgca ggcaaccggt gtgatcgtgc cgtcacgcat gcccttatgg atgccgtcca
     1321 cccgttcttt ctgagtaaac tcctcgccgg agaagaactt ttcaaacagt tcctcgtcag
     1381 tttccgcaat tgcctctgaa aacgcctgaa tcaagccgtc tacccgataa ctcaattttt
     1441 cggaaacctc ggaagtcggc atggggatct cctgagcctt gccgtccttg tcgtagcgat
     1501 aggctttcat ttcgatcagg ttgatgtagg aatccacctt ccggtctgca atgaccggta
     1561 ctactaccgg acaaaccgat ggcccgaaag cggatttcag ttctgtcagt acattataaa
     1621 agttggcgtt ttcatcgtcc agctttgtga ccacaaacat tcgcggcttg ccctctgctg
     1681 cacggtatgc ctttttcgtg cccacccgaa cgccggattt tccggatacg gtaatcatta
     1741 cgcagtccgc cgcagagata ccctgcacca tgccgccggc aaaatcaaac atgcccggtg
     1801 tgtccagcag gttgatcttc tgaccgtcca gcatgatgct gccggtggcg gtgtacacgg
     1861 aagaatgccg cttgatctcc tccggtgcag catcagaaac tgtagtgccc gccgcaacgc
     1921 tgcccatgcg ttccacgcag ccaccctgat acaaaagggc ttccagtaat gtggtttttc
     1981 cggtgccggc atggccagcc agtgcaatat tcttgatgat catagctttt tcgctcctat
     2041 cataaattct atttcgcagc aatttagcaa gttgcctaat tttatttcaa ctgcatattg
     2101 caattatagc acatttcaca tcacattgca agcccttttt gaaaaaagtt gaattttttt
     2161 cgaagaaccg gaaagtattc cgcctgtccg gtgtgtccga ctgtcaatat ttcttgtgga
     2221 gtttccacaa acttttgaaa tgctattgac aagtgcacaa aaaggtgata gaatatgatt
     2281 taaaatattt cagtcaacac tgtacaaagc cggcaaacgg actttatgca gcgtttcctt
     2341 tatgagagta actggaggaa atttttcatg gacgccgccg atgacagcga cacaacaaat
     2401 ccgaatcgaa tcatgatgac agcacgatgc cctgtgatac agcagaggta tggcattttt
     2461 tgcgttcgtg ctgcctgtag aaggcatagt ctgcaccgta tgcggtgcta gctgtgtctt
     2521 tttgtatttt ttaggcaata ccgcacaaag attgtgcgac agatgcgtcg tcaaattttt
     2581 cg
//
LOCUS       DAWBGR010000257         2580 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_9129_length_2580_cov_3.783762, whole genome shotgun sequence.
ACCESSION   DAWBGR010000257 DAWBGR010000000
VERSION     DAWBGR010000257.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9129_length_2580_cov_3.783762"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          780 a          586 c          590 g          624 t
ORIGIN      
        1 gcaggatctt gaagattgac cgctatggct ttctgaccta ctccaccttt gaagtgcaag
       61 aaagtttccg gttcggcaag tgggttgatt ggtacgacga gttggaagaa gagtattaca
      121 cacagcagga ggaactgctg ttggagatgt gcaattgcta cggcgtgaca gaagatgaca
      181 ttttcctgtt gctggactat ggttactctg ccgatgagat cgaggaaatg ctgtgcgatg
      241 cgacttttct tcatgatacg atttgtgcaa ttcagtgtca agaaagcaat acggcaatct
      301 tttaaatgtt ttggctgtct cagaaagtac actttttgag gcagtcattt ttttctcaaa
      361 aacctgtgcc aaaaggtccg tttttgactt ttgagacgtt ccaaaagtgg caggactttt
      421 tgagacaatt ggaggtgttt ttatggacaa cagaacgttc ggctatgccc gtgtttccag
      481 cagggaacag catgaggaca ggcagatcga ggctctcaca aattatgggg tcagcagaga
      541 taatatcatt gtggacaaat gctccggcaa agacactgag cgtgaaggct atcagtatct
      601 gaaaaagcag attctcagga acggcgatac actggttatt aaggaattgg acagactcag
      661 cagaagtaag tctgatatca agcaggagct ggagtttttc aagtgcaacg gcattcatgt
      721 caaaatcctc gacattccca ctacattgac cgattttccg ccggagcaga tgtgggtcat
      781 ggatatgatc aatgcgattc tgattgaggt gcttgggagc attgcggaaa atgaacgcct
      841 gaaaatcaga cgcagacagc gtgagggcat cgatgccgcc ttgaagaaaa atgtcagatt
      901 tggtcgtccg gcaattgcaa aaccggagaa ctgggacact gtagtcaaac aaattgatgc
      961 aaaagaaacc acggtttcac aggcgttgca gacactcaat atctcccgtt cctcctacta
     1021 tcggttcaga caaaataatc cattttaatc agatatacaa caaatccata aaataatcta
     1081 tggaggtatc cgatatggaa cgaaaaaaga gaaaccacaa cgaaggcagc atccgcgaac
     1141 gggcagacgg cagatttgag attcgtgtca cagcaggcta cgattttgag atcggaaagc
     1201 cgaaacgcat ctcctactat gctaaaacca aggcagaggc aattcaaaag ctgcacgaag
     1261 aggagtacaa gattcacttt cagaaacacg ttgatccaac gtcaaccacc tttctcgact
     1321 ggctgcggct ctggctggaa acctacatga aaaacaaggt caagcagtcc acctatgtca
     1381 gctatcgtgg ctacatcgaa aatcatcttg caccagcatt tccaaccctc aaactgaaag
     1441 atctcacgac aaagctcctg caggacttct acaactacaa gcagaatacg cagggacttt
     1501 cgcccaaaac cattctgaat cttcaccgct gcctgcacaa ggccatgaat caggcagtac
     1561 tggaacatta catcgatttc aacccctgtg atgcagtcag tttgccacgc aatgaaaaac
     1621 cgcaggtcga gattctcacc cgtgaggaac agcaaaagct tacctatacc agttacaaat
     1681 accgatacgg cattttcatc cgtttgacac ttgcaacagg gattcgtctg ggtgaacttc
     1741 tgggactccg ttgggaagat gttgatttca ataaaagaat gctctctatc cgccgcacaa
     1801 taaaccgtct gcccaaagtg gattacaacg gtgtgggcaa ttccacggag atcgtcattc
     1861 aggagccaaa aacgaaaaat tccattcgct ctatcccctt gattcccaat atcgcaagcg
     1921 aattacagca atggaaaaat gtacagcaga acgatgccat gacatctagt gcagcctatc
     1981 aggactccgg ctttctggtg acaaacccat ttggcggata cttggaaccc agaacattca
     2041 aggatgcata cgatgaaatt ctgaaagcct ctggtcttgg gcattatacc tttcacgccc
     2101 tcagacatac ctttgccacc cgagccatgg aacaaggcat ggacgcaaaa accacttcaa
     2161 ttctgttagg gcattcctcg gtttcgttta cgctggatac ttacacacac gttctggaca
     2221 gtcaaaagca agaggaaatg aaagtcatgg aggagttctt cactctgccc gatatgccgc
     2281 aagtacagtc ctatgcaatt gcagtaacgc caatggcaaa tggctttctg ctaaatcctg
     2341 tggactttga ggatatgagc attgaggcaa acgatctgca atacggcatt cagtgtctgc
     2401 aaaccgccat tgcccagaaa ttagccacaa tgtacccgcc tacgccgaca cctgtgaacg
     2461 agatcgtttt gcaacaaggt gaatttgtgg tgattgtcaa tttataaagg aaatggagca
     2521 ggatgtgaaa tgctcccctt ttgttagaca atatggtata atagaaatat accaattaaa
//
LOCUS       DAWBGR010000258         2575 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_9153_length_2575_cov_3.490476, whole genome shotgun sequence.
ACCESSION   DAWBGR010000258 DAWBGR010000000
VERSION     DAWBGR010000258.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9153_length_2575_cov_3.490476"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          767 a          509 c          693 g          606 t
ORIGIN      
        1 acgtgatact gcacaaagcg gtcttttttt gtttccggca gagatgaaaa agactgcttc
       61 aaaatacatt gcggaggaag aatatgaaga agttacattt taacagcaca ctgaatcaca
      121 aagcatcgga ttacaagcct tgtgaaatct gcgtggaaag ggtcgtcacg ctctatggca
      181 gaagctttga ggaattgaaa aatcatctaa tgcatgatga tccgtacatc gttgcatatc
      241 gtgaccttat gtacacggag ggtgatacgg cacattgtct gttgtttgtg gactatgaca
      301 gcggcgatgg agtactggtg gagtccgaag gcagcaatta cgccagaaaa tcacagttta
      361 tccccaacgc aagagcactg ctggagagca atgaaatgac cgcagctgaa acaaggctgc
      421 ataacgattt gaaaaaaatt gccgacaaag ttgcggagct tgcccattgt ggtgaaataa
      481 gcctcgcatt tgacgaactg ctggcagaat ccgatctgga tgtaaaatcc gttctgcgtg
      541 atgcggtaac agcaatgctc cgagaacgtg aggacattca gatggcagaa agtcagtcca
      601 tcgaagttcc ctttcagccg gatatcacgg tagaagcgaa accgacacag gaattgacat
      661 tttactgtcc gctaaaaatt gtcagagaat cggaagaacc tgattacgaa tgggacgagg
      721 aagttacaga ggaagatttt gaggaaattc cgtctgcata tgccgaaggc tgtgtggatg
      781 agatcaacga ttttattcgg aattgcgaag aaccggatga agaacaccgt ggattgatgg
      841 cttactatta cgatgatcct gccattcgag aaaaagtttt ctcggcgatt ccatccgtca
      901 gagatgttaa cggcgaactc gtaggcgtat ttgaatgtga aatttccggc gaactgacag
      961 taagtgaact ggaagattta cgcagctatc tcactggtca ggcaagcgat ggttggggcg
     1021 aaggtcttga acaacacggc gttaaaaccg ctgattttgg cgagatttat gtaagttttt
     1081 ggaatgacag caacgactgg tcgctgcaga cagaagaaga aatgggattg tctcaggctg
     1141 agaaacacac ggaagaaccc agcatgggca tgacgatgtg aaaggagcag ctatgtttta
     1201 tagcagagat aaagtggaac aactccgaca gaggtatccg gaaggcacga ggatttgcct
     1261 tgacagcatg gaaaatgatc cccgaccgat tccgtcaggc acaaagggga cggtaattac
     1321 agtagatgac gccggtcaat tattcatgaa gtgggacaac gggtgttccc tttcgctaat
     1381 ccccggcgag gacagattcc atgtgattca gccggaagaa aatttgtcgg aggaaatgca
     1441 agaaactgag aatccctcag aagaacttga aatgcatatg tctatgtagg cggtttgatt
     1501 tgaaaaaact gaactgtctt tttgcccaaa tgaaaggaaa aggtgaaaat gattcgatat
     1561 tttgaaagct tcgcaggcat cggcgcattc cgatcggcat ttgaaaaaat gggcggattt
     1621 gaatgtgtag gctggtgcga aatcgaccga tatgcgcaaa aggcttatcg agccttgtac
     1681 gacacaggag gagaaaagtt ttatgaggac atcacaaaaa tcgattacgg aggtatgccg
     1741 gattttgatc tgctcgttgg cggaccatgc tgccaatcat ttagtgtcgc agggcgcaga
     1801 ctcgcttttg cggacgacag aggaaacctc tttttcaact atatccaaat ccttgaagcc
     1861 aagcgacctc gctattttat tgctgaaaat gtacccaatc tgttgggaat atcgcagggg
     1921 gaatgctaca gaattatcct tgaaaagatt tcagaactgg ggtatcgtat gtgctggcgt
     1981 gtgcttaaca gctccggttt cggaatacca cagtcaagac gaaggctgtt ccttgtcgga
     2041 tatcttggag acagatgtcc cgccgaaata ctggctttcg gaggaaatga tgcggaaaat
     2101 tgcggcaaag gaaaaactga acagctgatc ggcggcagtc agggttcgag ggtgtattcc
     2161 gcaaacggca tagcggttac acagtgcagc ggttcaggcg gcatgggagg taaaacagga
     2221 ctttatttta tagactgtaa tccaagtcct caaatgacgg acgcagcaag atgtattact
     2281 gcaaggcaga acagcggaat aagtcatcac agaggtgaac attccgctgt ttttgtggat
     2341 gatgcgccga gagccattct caatccgttt aaggagcata cccgtcagaa cggcagaaga
     2401 atcaaagagc ccaacgagcc gatgtttaca cttacggtga cagacaggca tggaattgtt
     2461 cacagaggac ggattcgtcg gcttatgcct gtggaatgct ggaaattaca ggggtttcgc
     2521 aaggatcaat ttgaaaaggt tgcggcaacg ggaatgtccg acgctcagct ctaca
//
LOCUS       DAWBGR010000259         2573 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_9160_length_2573_cov_6.954329, whole genome shotgun sequence.
ACCESSION   DAWBGR010000259 DAWBGR010000000
VERSION     DAWBGR010000259.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9160_length_2573_cov_6.954329"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          719 a          715 c          579 g          560 t
ORIGIN      
        1 acttccctaa attttcaatg gcaaccttcc caaaaaaagg cccgaaacta cgtcaaatcc
       61 accccaaatc ccgcaagttc gaatcttgtc acctgacacg catgcaacct gtttggattt
      121 tccctgagcc tgcaacccct gcaggctctt ttttattgca aaatcgccgt ttttggaatc
      181 tccgacgtta tcccatcaaa tattcacaaa atattttcaa ttaaaagtgc aacatttgac
      241 cctcctcaat tgtgtctatt atagagggaa aaatatccgg agcactgccg ctggtctttg
      301 cagagaaagt ccaaactgtt gcattttccg aactctcgga caaatggaaa ggatttttat
      361 gaaaaaacaa ttcacaacac tactggcact ctccaccgca gcagcgtgta tgctgacctg
      421 cacaccgatc accaccagtg cagaggaatc tctcgatgat ctacagattt tctaatttaa
      481 aagggaggaa tttttatgaa aaaacaattt gcagcattac tggcactgtc taccgcagca
      541 gcctgcgttc tgagctatca gccggtacag gctggtgcag aaagcgttct gggcagcatg
      601 acacaggaaa atctgaccga catcaccaac gaacagaaag atgcgttcta tcagtcatct
      661 ctgggggaaa tagagccgtc ggcagaaccc agcgaactgg aacaggctgc cactgcaaac
      721 ggcatgtaca aggacacatt ctcgttcacg aaccactcct gcaacctgtc agaggacgac
      781 ttcaaccaga aggtattcga accgatctgg aacagcatga ccaatattga acggctcgcc
      841 tatctcaaca agagcagtgt ataccggaac gtctgccaag gcagccagtg cttcggcatg
      901 gcagccctgt ccattctggt acacaacggc gaactgtccc ccagcgacat tcaggcaggt
      961 gcagaaaccc tgcatgacat cacgctgacc aacgacgtag aacagctgat cacctactat
     1021 gacatgctgc tggaatatca ggcagtttcc aacgtggtca tgtatgctcc gacactgctg
     1081 acacaggacg aacgcgtaga caacctgctg aactgtgcag aacgctgcaa ggaaaagggc
     1141 acttacttca tggttgccat tcgggccggc ggtacgcaca tgatcgtagg catggacaag
     1201 gtttccggca gctggacatt tgacggcgtg aactacgaca cctgcattgt tgcctacgac
     1261 agcaacttct acaacaagac caccaaggaa tcgctgttca acgacaacgc ctgcatctat
     1321 gtcaactcta agaccaagca gttctgcatt ccggcatacg ggttcacttc agaaaagggt
     1381 gaatctgtac tgtatgcttc cgatgacgat tccgtattga cctatcgtgc accgctgaac
     1441 ggcaccacag agatcaacac cgatgcttcc aatatcgtaa atctgcattc ttaccgcggc
     1501 atcacaccgg aggacgtatc ggtcactgcc accgatgcag acggcagcac ccacgacttt
     1561 ttgagcgaat gcaagctgcg gtcctcggat tccaactact acggcaccgg ctctgcattc
     1621 catctggaaa agaccaagcg ttccaacggc tttgacttct acctcaaggg caagggctac
     1681 cgcgtagaag tatcgcagta tccgcagggc ggctccatga gcagccccta cgacaaaaga
     1741 acgtatgacg tgggaaccaa gtgcaaggtg gacatcaccg gcgacagcat ttcctacacc
     1801 aataccgaca ccgcaagcct gccgcttgag ttcagcatgg cttatgacga gggcaactac
     1861 aacttctctc cgctgaacgc cgccaaggtt acctttgacg tagcaccgaa tcagacgatc
     1921 accattacca agaaggacac aggcttcgca ctcaccacca acatggaaac ggttgttacc
     1981 attaatccgg agtacaacga tgatgcgatc aacgcattcg agggcagcga acacgacttt
     2041 gtacagtatg tcacagctta ctacctgcgg tttgtcatgg agagcaacgc catggtcaac
     2101 tacaacatgg aaaagaagtg cagtgaactg tcattcgact tcaacaatga cggtgtgtac
     2161 gacgagtccc ccattaccgg cgatgcagac tgcaacggtg ttccctatga gatcaacgac
     2221 atctactgga ccatgcagca gattgccgaa tctggtgcat acggtgccgg cagcacctac
     2281 ttccccacca cagccggcga cattgacggc aacggcaagg tagacaccaa cgacctgttc
     2341 gatatcacct atgagtgtgc tctgcgtggg gcaggactga agaagtaact ggtacttaca
     2401 ctaaggaaac actgcataaa gcccgcctga cggctttgca cgcaatctgc ttgcagattt
     2461 tccgtcatat tgcatagaaa tcccttgcca tacggcagta tgcctgcgga atttctatgc
     2521 aatctgccga aaaatctgac tacgcatctt acgcacaagc tttattcagt gtt
//
LOCUS       DAWBGR010000260         2567 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_9180_length_2567_cov_3.333201, whole genome shotgun sequence.
ACCESSION   DAWBGR010000260 DAWBGR010000000
VERSION     DAWBGR010000260.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9180_length_2567_cov_3.333201"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          624 a          568 c          591 g          784 t
ORIGIN      
        1 gtgaaacagc tggaggtgca agtttagctg ttgatttgcc tgaatgttct ttttttatta
       61 tatcatgatg aatgcttttt gtcaatcctt gtgagtcgag ttttctcggc tcattttttg
      121 ttgacttgaa aactgccagg tcggcaatgg ttcgtgctcg tcggcgaatc cgtcggcatg
      181 aaatggcaga atgcccattt ttgataggac ggggctgaaa acgtccgaaa agttctggat
      241 gcctgcgaca tttttaattt tgttggtgga gtgggacttt gacattaaaa ttgaaggtta
      301 gaatgctgat ttttctgaaa attgaatatg gttgtaaaag ggaacatctc tttcctcacc
      361 accccaccca gtgaaaaaag tgataccctt ccccaaaccc tctataaaat cttcttccac
      421 ttccgaaaaa aatacctgga atcccgttgt tggaattcca ggtatttttc ttatgcaggg
      481 ttctgttcaa acagagcagc atccagcttg tcggcggctt cctgttcgac ttttttcagc
      541 acatgactgt aaatgtccat ggtgatctga tagctggaat gtcccagccg ctcctgtgcg
      601 accttgggag agataccaag cgaaagcatg atcgtggcat tgatatgccg aaggtcatga
      661 aaccgaatgt gcttcagatc gttggctttc agaaatcgcc gaaacttcat ggaaaaggaa
      721 tcgcttttgt agggagagcc gtcctcctgg cagacgatgt aatcattttg tccggcgtgg
      781 cgttcttctt tggtgttttt caacagccgc agcagcgttc cgggaatctg aatctcccgt
      841 ctgccgcttt gtgttttcgg ggctttggtg atcgtttgat tgttgacagt taccagattt
      901 tcttcgacag taagacagcc ggtttgaaaa tccacatgat gccaccgcag tgcgagcagt
      961 tctccacgcc gcagacccaa acaaatctcg atcatcagtg gcagataaag ggcagtgttt
     1021 ctggcacatt gcagcagggt ctccacttct tccatgctgt acatttccgg ctggtaatgt
     1081 tccagcttgg gcagtgtgag atcgtcacag ggattcttcg gaatcagttc cagcatgact
     1141 gcctttttca ttgccgccga gagattcaac ataatgtttt tgatcgtttt gggcgaaagc
     1201 ggctttcctg ttctgggaga tgccttttgc agggaaaaga cccatttctg aatgtcgatg
     1261 tttttcagtt gctgcaaaca ggtatctcca aactgtggaa tgatgtactt ttccgtctga
     1321 tcgatgtagt gctgtacagt ggtaggggag agctgatttt ttacgtagat ctccagccac
     1381 tggtgcatga aatcccgaac cgtgatccga ttgtctttga caaaagttct ggtttccagt
     1441 tcatggatca tatcccgcat gactttttct gcttgttttt tcccgccctg aatggtcttg
     1501 taatagcgtt tgcgttttcc gttggcaaaa gaggattctt cttccacgat gacctgatag
     1561 gttgtgatcc cggatttggt cgtccgcttt cgcacatgac cggtatatgg cataaaaccg
     1621 cctcctttga aaagtataac agaatgttct tcgggctttt cttgaaaaag acggaaaaag
     1681 attagtattc ttctgcaaac agcatggtgg catagggctt ttggtttgca tcatattctg
     1741 caatcacata gattttcgct gtgaccggcg aaaacggcac ggcaaactga tagcattttt
     1801 cataaggcgg aacttcctga caatgcaaaa tgatctgctg gctgcctgct ccggaaagcc
     1861 gaaatatttg cagataatcc ttgggaacag gcagttctgc aatgcagttc cagagaaata
     1921 attgcagaaa aagcggtagt tcctcatgga ttctttttgt cagatagcgg tttcccgaaa
     1981 acatcagcag catcttcttt ccacaagttt ctgtgcttct gtggaaaatg ggatgtagat
     2041 atcctcgctg tatccgtccg aagatgccca gacagtgtgg atccagtcag agtgggaaaa
     2101 ggaaaggatc tcatagaatt ccaaaccttt gtcgagcaga taggatttct cctgtttttc
     2161 taagagaatt acagaaagga tatcacccat gtcacagtga taggtttttt cgatgttttc
     2221 cattttctgc accagatatg atttgagatc tgacggcatt gcagcatttg aaatagttgc
     2281 aatagagtgt aatttttgca tgattttcct ccaattttgt ttcaaaaaaa gaggctcagc
     2341 gtcatgccaa gcctcccgca aacagaattt ttacttactc ttcttgacca gtgccagcag
     2401 ttctacgccg caggcgactc ctgccagaaa catttcaatt gcaatcggca ttgatgtact
     2461 tcacctcctc ccgttcctgc atttcgatgg cgatcccgat gccgccgatc accagtgtca
     2521 gtaccagtcc tgcgataaat tccttcatgt caaattcctc caatttt
//
LOCUS       DAWBGR010000261         2544 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_9295_length_2544_cov_4.784251, whole genome shotgun sequence.
ACCESSION   DAWBGR010000261 DAWBGR010000000
VERSION     DAWBGR010000261.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9295_length_2544_cov_4.784251"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          700 a          588 c          709 g          547 t
ORIGIN      
        1 cgagcatttt tgtgaaatat gacgggaaat ttgcaagcag atggcgtgca aagttgtcag
       61 acgctctttg tgcgatattg ccttaaaata tatgccgaaa tgtagacgat agggggtatt
      121 cccgtgccgc aggacgaagc cgcgtttgcg aagttttatg cacggcatgt cgatacggta
      181 tatcgtatgt gctacatgcg gatgaaaaat cagatggatg cggaagatgt gacgcaggac
      241 gtttttttgc gagcgatgca gcaaccgcag ctttggtcag atgatgacca tgccagagca
      301 tggctgttgg tgactgcgtc gaatctttgt aaaaatgcga tgcatcactg gtcacggacc
      361 aagcgggaag aagtcgaaga ctgggaggca actcttggaa caacggcatc gccggatgag
      421 gtttctgctg tgatgtcggc agtaatggga ttaccggatc aatacaagac cgtagtgtat
      481 ttattttatt atgaagggta ttccggagca gaaattgccg gaatgctggg aaaaaaagaa
      541 tccacagttc gttcgctgct gcaccgcagc aagaagatgc tgttgaaaaa gttaggaggt
      601 gagccgtatg cagaaacagg aagatgaaaa gctggaacgc cgtctgcatc aagtgatgga
      661 gcagatccag cctgatgaag atgcgaaaga ccgcatgtat ctggagatca tgcggcgtgc
      721 ctctgttgct gaaacaccgc cggcaaagcc gaagccgtgg tatctccggt ggcagaccag
      781 tgccggagcc tgtaccgcag cactgctctg tgtcgttgtg gtggcggcgg cagtgcacgg
      841 gagaatgccg cagggcggca ccggtcaact ggatattgtt tctcccacag tgacgacaca
      901 ggcttctgaa ctgccggcag acacggagca gccggacgaa acagaaacaa gaccgcagac
      961 agaaaccact gccgcagaaa ccggcgtgac agtgcactct gcgggaaatg ccagtgctgc
     1021 cgcggaaagc agtactgttc cggcggaaga tgaaatcgtt tctccggttt ctccggaaat
     1081 caccgtgccg cagaagccgc agcagcagga aacccagccg gtcgtgacga ctgcggctcc
     1141 gcagaccact gccgcagcaa cgactgttcc gaaagcggaa accactgcgg caccggaaac
     1201 ggtcaccgaa ccgccggcaa ccacagcatc atctatggaa actgtgccga tccggcagaa
     1261 catctacctg tattataagc tgacgtggga gggaaatccc tacgatacag attatatgga
     1321 agtgtccagc agttctctgg atcagtattt gggatacggc gtgacacggg gcaatgatgt
     1381 ggaagatacg tataccgtgt tgatctactc tattgttggc gtggatcctg tacagcaggt
     1441 agcagtacag tatgcagggg aagaaaagta ctatgttttc acgatccagt gaatataaga
     1501 gcttattgaa aaaatgaaat ccggaatcgg tgtcactaat ctgccgtggc accgaatttt
     1561 ttttagggca atactgaata aagcttgtgc agtgttgttt tatcggaaat attgtcttga
     1621 caaatctgta aaaaaacagt atactagaac aggaacgctg aatatgcggt atttttttct
     1681 gataacgggg gtatcaaatt ggcaaccaaa cgagtaaaac aactggaaaa attcctgatc
     1741 ggactgggaa ttgctttgct ggcgatgatc atcatctgca ttatcgtggt cagccgtgcg
     1801 gacaaggttt ctgcaccgga cgcgattccg attctgacga caacgacgac aacgacaacg
     1861 acgacagtca ccacgaccac gacgatcaca tatgattaca ccatgcacat cgatatggaa
     1921 aaggtcaagc agcatcatga gaaaaatccg gacgttgtgg gctgggtcta tgtcaatgat
     1981 acacccattg attaccccat tgtacagaag gacgacaatg tatattatat cgaccgtgac
     2041 tgggagggca atcacagctc tgccggcagt atcttcgagg acttccgcgg caagatcggc
     2101 gaaacggaaa acaccctgct ctatgggcat aacatgggaa atggctccat gttccactcg
     2161 gtcaagagtt tcaaggaaga agaatggggc agacagcaca tttatttcga ggtggcaaca
     2221 ctggacaagc ggtatctgta ccgcatcgtg gcatcttccg tgctgaacgg tgaggacggt
     2281 acagcattcg actactggaa ccgcatcact ctgaatcagg aggaattcca gaagtacatt
     2341 gaggagattc gcagtacggc gttgatctgg tacgcaccgg acgacgaccc gcctgcatac
     2401 ggggacaaga tgattgccct gcaaacctgt aactccggtg cagacgacgg aatccgctgc
     2461 ctgctgttcg gacagtgtct gggcgaattc taaggcaaca ccgcacaaag cccgcctgac
     2521 ggctttgcac gcaatctgcg tgca
//
LOCUS       DAWBGR010000262         2531 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_9357_length_2531_cov_4.246365, whole genome shotgun sequence.
ACCESSION   DAWBGR010000262 DAWBGR010000000
VERSION     DAWBGR010000262.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9357_length_2531_cov_4.246365"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          542 a          728 c          628 g          633 t
ORIGIN      
        1 tctgggcaat atgacggaaa atctgcaagc agattgcgtg caaagccgtc aggcgtgctt
       61 tattcagtgt ttccttaagg actataaaaa aataccggat accgtatcga tcgaatacag
      121 tatccggtat ttttatgccc gcagcacctt gtccagcacc acaaactgct gttccttggc
      181 agtcagacaa atttcatctc ctgcctgtaa cgcattgtgc agcatcttgt cggcaatggg
      241 attctccacc atggttgcca gatactgctt catggggcgt gcaccgtact ggcggatttt
      301 cggtgcgttt gccagaagcc gcactgcgtc cggtgtatac tccatggcga tttccatatg
      361 agagagccgc tgctgcaatt cctgtaacat gcgttctgcg atctgggaca agctttcctc
      421 cgacagcttc tgaaaccaca gtacaccgtc cagccgtccc agaaattccg gcgagaaatg
      481 ccgcttgagt gcatcagtgg cagcagcatt tccgcccttg ccggaatcgg ggagaaatcc
      541 cagctgttca ccagtacgca acgcttcgcc gcccagattg gaggtcataa tcagcaatgc
      601 atggcgaaag ctggtcgtcc tgccgcagga gtccgtcaga atgccgtcct ccatgatttg
      661 cagcagcaga ttgcacacat ccgggtgagc cttttcgatc tcgtcgaaca gcactacgca
      721 ataaggtcgc cggcgaaccc gttcgcagag catgccgccg tcctcaaatc ccacatatcc
      781 gggaggagca ccgatcagct tggaaacggc atgcttttcc atgaattccg acatgtcaat
      841 acgaatcagg ctttccttgt catcaaagag gtgtgccgcc agagcacttg ccagagccgt
      901 ttttcccacg ccggtgggac ctgcaaaaag aaatgccccg atgggacggg acttgtcctg
      961 taagccggtg cttgcacggc agagagccgc cgacagtgtg tccactgcat catcatgccc
     1021 gaccacctgt tgttttagta cgctgcccag tgtcagcagc ttttgccgtt gtgcctccgt
     1081 gattttttcc gtgggaattc ccgttcgcac agacacaatg gcggcaacat cgtcctcctc
     1141 cacatcagcg atttccgtgg tgtccttttc cgcctcccat cggattcggg cacgggagca
     1201 tgcttcgtcc aacaagtcga ttgccttgtc gggaagaaac cggtcgtgca gatagcggtc
     1261 tgcatagtga attgccgtgc ggagaacggt gtctgaaatg tgcacccgat gaaattccgc
     1321 ataggtttcc cgcaatccct gcagcatggc gtagcattgt tccgcagagg gttcgtggac
     1381 tgtgaccggc tgaaaccgcc gttccaaggc accgtccttt ccaatggtcg atcggaattc
     1441 ttccggtgtg gtggcaccga tcagccgaat ttcacctcgt gccagctgcg gtttcagcat
     1501 gtttgctgca tcaattgcac cttctgcggc accggctccc acaatggtgt gcagctcatc
     1561 aataaacaaa atgacattgc cgtgctccga agcctctgcc agacagttct tgagccgttc
     1621 ttcaaagtcg ccccgatact ttgcaccgga cagcaatgcc ggcagatcca gtgcaaacag
     1681 ctgcatttgc tgtaaggact ccggcacgtc accctgtaaa atttttctgg caatgccctc
     1741 cacgattgca gtttttccaa cgcctgcttc tccgatcaga caggggttgt ttttggtgcg
     1801 tctggagaga atgcggatca tctgctgcaa ttctttttcc cgtccgatca gcggatcata
     1861 gcggtaagga tttgacggat ctgtcatcag ctttccgtac tgaaacagtg ccggacaatt
     1921 ttttcgggaa agggtatcca tacggtttcg ttccattcgc accccatagg cagtgtcgct
     1981 gctgcaaagc gtgcagagct ggttcagact gacacccaac tttttcagaa tggtgactgc
     2041 actggaacag ggggcgtgga taattgccag cagcaaatgt tccgttcccg ccagacagca
     2101 ccccgcatct ctggcacgtt ccttggcatc ttccagaatt gccaccagcg aaggggtcag
     2161 acaatcctcc tgtacctgtg caggaatccc gcgtcccacc agatcgacaa tgccgtttcg
     2221 cagccgttcc gtgttcagct gctgtgccgc caatgcacta gcagccgcat tgccgccctc
     2281 ctccatcagc cccagaagca ggtgctcact tccaatatac gtgtgcccga actcctccgc
     2341 ggcgtgtatc gcttctttca tggcaaggat cgccttgccg gtaaaacctt ctgtatatcg
     2401 catgttgccg ttcctttctg ctgtgtgttc acactttatt tcttttgaac gggacaagta
     2461 tagcatattt tttctgcaag atttgtcgaa gcagtcacac tttttctcca cgggcatatg
     2521 atgtactatg a
//
LOCUS       DAWBGR010000263         2512 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_9448_length_2512_cov_3.493691, whole genome shotgun sequence.
ACCESSION   DAWBGR010000263 DAWBGR010000000
VERSION     DAWBGR010000263.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9448_length_2512_cov_3.493691"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          545 a          714 c          663 g          590 t
ORIGIN      
        1 atgcggcagc gttcctccag cgaaagggtt tcctgctggc tgtcctgcaa tactgccagc
       61 cgtgcatctg aatgaaacag gatcctgcca aatgtaaagg ctccgcgttt gagcatctgc
      121 cgcagttcct gcttccgctg gcgagaaaag ccgtccatgt ccctggcgta ctgggcatag
      181 gaatagcgtt ccgccgtact gccgtgccga aggatcagca tgtccccctc ctgcaacgca
      241 agcttatact tgcgggaaaa ttcttccaga cagcggtgct ccacgaaaca gaccaccccg
      301 aaccccagca gcttttccgc cagtttctca aacggaaata ccggcagttt ctccgtcgtt
      361 tccgcctttg ccggcgaagc tgcttccggt ttgagtttca tcttgctgct gacatgccgc
      421 ttgacacggc ggttcagcgg ctgctgtaga tttttggtca cagtcgattc cgcttcattc
      481 atctgcacat tgcggtcata aaagtgcagc atgtcgttat gggcagaaag ctctgcggaa
      541 aaggactgta gtttcggcac agctgaaacg tccagctgct ccagatccag ctttttctgc
      601 actgtaccgc tgtccgccac aacgaccaga ggcatgtcga aatctgctgc gtgacagaga
      661 gcatcaaagt aggcaatgtc gcttttcttc cgaatctcca tggcttttcc gttcagcaga
      721 aaaccgtttt tgagaaatcc cacatgggga ttctgtgcca gtgccggaat gatcatagga
      781 cggaacactt cacagggagc cgtgctgtct gacggttcag aacaaatggt gcggacaccc
      841 acttccacat cgtccttgca gagcaggaaa gagatctcgg tttcaaaaat tctgccgctg
      901 accgcaggac gttcctgcgg agtacccaga ttggcaccaa tgtccggctc agtgatctga
      961 aacgaccaca gcccgcggtt ttccagataa atcacatcga cagccgcccc caccgaggag
     1021 tgaaacgagt gcagctgctg catggaaaag gcttcatatt gtgccggctc cggtgtgcag
     1081 atgtcctccg gcaggctgct gaattttttc aggcgggaac ggagccaatg cagggtttcc
     1141 agcacacaga tgcagaaaac ctgttccgca ggcagttttt tctgtgccgt tctggcgtgg
     1201 aattgatagg taggataacg cttggtgggc tgtaggttgg tagtctgata tttcatgtac
     1261 atctctcctt ttccggaatg atttatgcgg tattgcctat tgtaattttt catagggagc
     1321 gattcccacg gcatcgaagc cggcgatctc gccatatgct gccagtgcaa agtcgaacag
     1381 tttggcaccg atcacgccgc cggtgccctc tcccagacac atgtcgcagt ccagataggc
     1441 acgcagcccc agtgcctcca gtgccagctt tccggcaggc tctctggaac agtgagaggc
     1501 gaacagatac tgccggcaga gaggaaccag ccgcacggca caattcgccg ccaccgcgga
     1561 aataaagccg tcgatcagca ccggcacatg gtagactgca ccgcccagaa atgccccagc
     1621 catgccgcag atgtccagcc cgccgacctt ggaaagcaca tcgagcacat cgttcctgtc
     1681 gggctggtgc agggcaatgg ctttcgcaat gacttccacc ttgtgccgca gtcccgcacc
     1741 ggacagaccg gcacctcgtc cggtgacctg ctccggcggc agctgctgca aaacggaaaa
     1801 gaccgcactg gaagtggtgg tgttgccaat gcccatttcg ccggtgatga tcaggttgta
     1861 gccggcggcg accttttctc ccaccagtgc aatgccagtg agaatggcac gttctgcctg
     1921 ctcccgcgtc atagccggct catgggcaaa gttgcgggtg ccggcggcga tctttctgga
     1981 aattatgccg tcgcagtgca gttcctccgc cacgccgatg tccacgggaa aaacatctgc
     2041 cccgcagaca cgggacaggg cgttgactga agcgatgccc atggtaaaat tgcgggtaac
     2101 ggcggcagtg actgcctgtg tggtctgcgt cacgccctct gccacaacac cgttgtctgc
     2161 acagaagatc aggactgcct tttttcgcgg cggcaggttc gccgtaccgg taatgcctgc
     2221 cagctgtacc accagatctt ccagccgccc cagactgtgc aggggcttgg caatcgcgtc
     2281 ccagtgctgt cgtgctgttt ccatagccgc ggaatccagt ggtgagatct gctgtaacat
     2341 ttcttgaagc atgggagttc cctctttctt ttcagtattt cctttcagtt cttgttttca
     2401 ttatagcacg ttttgtcgat ttgtgcaagg cattgaaaaa gtggaacttt cccaaaattc
     2461 aaaagaaaac atataatttt tcctatattc tattgacttt tcagatatga gt
//
LOCUS       DAWBGR010000264         2489 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_9556_length_2489_cov_4.269926, whole genome shotgun sequence.
ACCESSION   DAWBGR010000264 DAWBGR010000000
VERSION     DAWBGR010000264.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9556_length_2489_cov_4.269926"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          652 a          621 c          633 g          583 t
ORIGIN      
        1 atatgacgga aaatctgcaa gcagattgcg tgcaaagccg tcaggcgggc tttatgcagt
       61 gtttcctaaa gcaccgctgc atcagcatat cgtctatcat taaaaaaagg agttttacgt
      121 atgaaaaaga actggacaat ccgcctgctg gcaggcgttg cagccggagc aatggcattt
      181 tccatggtgg gctgcggcag cagcaaagac aattccaaaa acggcggcaa ggaaaaattc
      241 attgtaggct ttgatgccgc attcccgccc tatggctatc aggacgaaaa cggcgaatat
      301 gtgggattcg atctggatct ggctgcggaa gtctgcaagc ggaacgactg ggaactggtc
      361 aagcagccca tcgactggaa cgccaaggat atggaactga attccggtac gatcagctgc
      421 atctggaacg gcttcaccat gaatggccgt gaggacgaat acgcatggac agaaccgtat
      481 gtagacaact cgcaggtatt cgtggtcaag gaggacagcg gcattgctac ctttgatgac
      541 ctcgccggca agagcgttgc tgtacagacc gattcttccg cagaggctgc cctcaaggaa
      601 gatgacaaca aggagctgaa ggattctttc aaggaactgg tagtcgtttc tgattacaac
      661 acagccttta tgaatctgga ctccaatgca gtagaagcca ttgccatgga catcggcgtt
      721 gcaaagtatc agatggaatc ccgcgacggc aagtacaaga ttctggacga cgtgctctcc
      781 gcagaacagt atgcagtggg cttcgccaag aacaacaccg agctgcggga caaggtacag
      841 aaaacactgg acgaaatgcg tgaggacggc aagtttgcag agatcgctga aaagtgggat
      901 ctgacggatt ctgttattga ctaaatctcc gaaaggaaac ctgatatgtc tttttttgaa
      961 atcgtcgcac aattgagcga cagcatgctg cgttccacat ggatcttcct gctgacactg
     1021 gtattttcca tgccgctggg acttctcatc tgtgtggccc gcatgtatgc atggaagccg
     1081 gtacagtggc tgatgaaatt ttacatttct gttgtccgcg gcacgccgct gatgttacag
     1141 ctgctggtca tctatttcgg aaagtatttt ctgttcggac ttcccctgac gaccaattac
     1201 cgcttctggg cagtaatcat cgctttttcg ctgaactacg ccgcttattt tgcagaaatt
     1261 ttccgcagcg gcattcagtc cattcccatt ggacagcacg aagctgccaa gattctgggg
     1321 tacagccgca tgcagaaatt cctgcggatc attaccccgc agatgatcaa gcgagtgctg
     1381 ccggcagtga ccaacgaatc gatcacactg gtcaaggata cttccctcgc ctttgcgatc
     1441 tcctacattg aactgtttac agaaacccag atgatcgcgg cttcggaaac ctccatgctc
     1501 ccctatctgg ctgctgcgat cttctactat gtcttcaatc tggtagttgc cggtctgatg
     1561 tccctgctgg aaaagaagct gaactactac aagatttaag aggtgccaca ttgaattact
     1621 tagaagttga acacctgcaa aagcattttg gagaggtgca ggttctcaag gatatttcct
     1681 tttccgtaaa gccgggagag gtgctctcca ttatcggtcc ctctggctcc ggaaaatcga
     1741 cactgctgcg atgctgcaca tttctggaaa cgctctccag cggaaaaatt tcctacatgg
     1801 gacaggaggc agtgcaggcg aaggacggca agcgtccggt ctatgccccc aaggcggaat
     1861 tccgcaagat ccagcactat ttcggtctgg tattccagaa cttcaacctg tttccgcact
     1921 attccgtgct ccggaacatc accgaagccc cgcggctgat cctcaagaaa agccgtaccg
     1981 aagcagagaa aacagcaatg gaactgctga aaaaagtggg tctggctgac aaggcaaaag
     2041 cgtatccctg cgaactgtcc ggcggacagc agcaacgagt tgccattgta cgtgctctgg
     2101 caatgtcccc gtcgatcctg tttttcgacg aaccgacttc tgcactggat ccggaactga
     2161 ctggtgaaat tctggaagtc attcgtctgc tggcagaaga tcacatgaca atggtcattg
     2221 ttacccacga aatgagtttt gcaagagcca tttccaatcg gatcatcttc atggcagacg
     2281 gttacattgt tgaagaaggc acgccggaac agatcttcga gcgtcccgca caggaacgca
     2341 cccgtcagtt tctggcacat tatcagtaat aacagaaaac gcaagaatca gaaaacacca
     2401 gccgcaggca atccgtattt gccagcggct ggtgttttta gggcaatacc gcacaaagat
     2461 tgtgcgacag atgcgtcgtc aaatttttc
//
LOCUS       DAWBGR010000265         2466 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_9665_length_2466_cov_5.246371, whole genome shotgun sequence.
ACCESSION   DAWBGR010000265 DAWBGR010000000
VERSION     DAWBGR010000265.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9665_length_2466_cov_5.246371"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          690 a          520 c          564 g          692 t
ORIGIN      
        1 gtttcgtatc tgacgcatgc agattgcgtc gtcagatttt ttgagagatg aaggggaaaa
       61 cggagaaaat cctttgtgga ttttcgagta ttttcccgaa atatctcgga aaatatgcac
      121 gcaagatgcg tgcgggaaga tacgaaacag gctctgaaga gaaatttgca agcagatggc
      181 gtgcaaagtt gccagacgct ctttgtgcgg tattgccttt tgttgctgcc gcatgtctgc
      241 ggcgaaaaac gaggcaggaa tgcatctgca ttctgcctcg ttgtaaaagc ctgtgttgcc
      301 gtatgttatg tcctgtgtgt ttatacgtta aaccggaaca gcacaatgtc accgtcctgc
      361 atgacatagt ccttgccttc cagacgaacc agtccttttt ccttggcagc cgccatagaa
      421 ccgcatgcca tgaggtcatc ataggaaacg acttccgcac ggataaagcc cttttcgaaa
      481 tccgtgtgga tttttccggc agcctgcgga gcctttgtgc ccttggtaat ggtccacgca
      541 cggacttcct gcttgccggc agtgaggtag gagattagtc ccagcaggga atagccctcc
      601 cgaatgatac ggttcagacc agattcttcc agtcccagat cttccaaaaa cattgccttg
      661 tcgtctgctt ccatgtcggc aatgtcttct tcgatcttgg cacagatcgg cagcactgcg
      721 gcatgttcgg cttcggcaat tgccttgact tcctgataat gctcgttctt gtccacgccg
      781 ttgcggaaat cttcttcgct catgttggca gcgtagatga ccggctttgc ggacagcagg
      841 ggcatgtcgt ggatccagct caactcgtcc tcggtgaaat caaaaccgcg ggcagacttg
      901 ccttcttcca gatgtgcctg caaacgctcc agaaagtcca cctgtacctg taatttcttg
      961 tcacccttga gggctttctt ggtgcggtca atgcggcgtg ctaccatttc catatcggag
     1021 aagatcagtt ccagattgat ggtttcgatg tcggaagccg gattgattct gccgtctacg
     1081 tgaatgatgt tgtcgtcctc gaagcaacga accacgtgta caatggcatc gacttcgcgg
     1141 atgtcgccca gaaacttgtt gcccagacct tcgcccttgg aggcaccctt taccagaccg
     1201 gcaatgtcca caaattccag cgttgccggt gtgaacttgt ccggttcata catttctgcc
     1261 agcttgtcca gacgagcgtc cggcacagaa acgataccca catttttttc aatggtgcag
     1321 aacggatagt ttgcggattc tgctccggca tttgtcagag cattgaacag cgtgcttttt
     1381 cccacattgg gcagcccgac catacctaat ttcattgtta cagttccacc tttttttcaa
     1441 aattatccgg catttgccgg cagttgacat acactcattc ttatagttta gcatacaacg
     1501 ccggatttgt caagaaagca attttcaaat tcggaaaact tagagcttgt gttcatagcg
     1561 aaaaagtgat ataataggag acatgaaaaa agagtatgaa gcaacaaatt acgaaagtga
     1621 tttgacagac gagcaagggg aagcaattaa aaaaattttt cttggatcaa aatgaactaa
     1681 gaggcgacat taatactgtt gcagatatcg taagacaaag aaaaaaagag gctaactcgc
     1741 catgaagaaa ttaaagtgtg tttttatcat tatatttcta atcatagcat ttacaaccgt
     1801 tgtatctatt tcaaaatgcc aagaaaacga acttggttcg gtagctcaag gtataataga
     1861 tgagatcgaa acttcgggaa acgatgaatg tatggtcgaa ttgaaaaccg ttaccaattt
     1921 tgaatgggat agagcaatta ttgcttcagc tgattttttc gcaatgggat attcttctga
     1981 aacactggaa gatatttgga aaattgagta tgatcctcag tcagatttta gatcaaggat
     2041 tttatttgta aaagataata aagtagtata tgaagaatca tatgcggcat ctattgagga
     2101 gtctgcgaaa tttaatattt cggtatcacc atctctggat tattattgta tattgccata
     2161 tgatgattct tttgcatttg ttacaaaaga aaagcacaaa tcaagtactt gttacagttt
     2221 aataatctac ccaaaaaata aaatgaatta gagcttgtgt tcatcggtaa agtatgcgga
     2281 ttttcgagca taatttttat gctgacaagg aaaatatccg caggaatact gtcgtattgc
     2341 aaggatattt gacgaagttc agcaggaaaa tttgccgaaa agacgtgtgc tgaaccgatg
     2401 aacacaagct cttagtacag gataccatgg aaaaacagtt gatatggaga agtgcagtga
     2461 caagca
//
LOCUS       DAWBGR010000266         2461 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_9684_length_2461_cov_3.001247, whole genome shotgun sequence.
ACCESSION   DAWBGR010000266 DAWBGR010000000
VERSION     DAWBGR010000266.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9684_length_2461_cov_3.001247"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          579 a          645 c          475 g          762 t
ORIGIN      
        1 gccagcttcc tttaccagat aatctgcaaa gttcttgatg tccgactttc cctccagctt
       61 tgccagaaac gggattacaa tcaatctccg ccacgttccg tcatcattcg caccgactct
      121 cggcagatga ttcgtgtata acaccagcgt atgagcaggt gtatagcgga acgggtcttt
      181 gtatttcttc tccgcttgga tttcatcggt cgagcaaagt tgcttgatga cggcagtatt
      241 caaccgcatt ccttcttcca gttctgctgc aatgaccagc cgtttgcctt ttagttccgc
      301 catctccggc tttacatttc gcttgcagcc taccgtcaat gcatccgcag acattgtccc
      361 actgtaacta cccagcaccc gtgacaccgc attccagaat gtcgacttgc cgttgctgcc
      421 ttcaccgtaa gcaataatca atgcttcttg atacacctta ccaatcgcac aaagtccaca
      481 aatctgctgc acataatctg ttagactttg atcgccacag aagaaacaat gcaaagcctc
      541 ttgccagatt tctgcaccaa cattgtccgg cgaaacagca gtcattttag tgagataatc
      601 ctctgggttg tgcgaccttc cgccatacac gcctttttgc aggtcgtagg ttgccgctgg
      661 cgtattcagc aggaattcct gactgtcaaa gtctgcaatg tctttcagca gcatcggttt
      721 tgctgcttgt aatgccgaag agatgtactt catatctctg cgtttcatga cgaaagttcg
      781 gtaagtcagg gcagaacgat attcgatgta cgcttttctg ctgacatcat ccacagcttt
      841 ttccagcacc tttccgccct tggagattgt ttcggcatcc actccgctgt ccagcagcat
      901 cttgtgtgtc atttccagtg ttttttctgc ttcctccagc tgcgtatcca gaaatgcttc
      961 gcatcttcca acagcagtct gttttgattc tacccaatgt gtctgaagat agcagaggta
     1021 ttccgttgca tcggtgtagg caagttctcc ttgtacctgt ccagcaaaaa ctttcgcttg
     1081 cccgatgtct gaatagtcct ccggtcgcag gctatacatc tgcccatata actctggagc
     1141 aatatatccg tcctgtttgg atactcgctt tccgaaattt tttgcactct gccaaatcat
     1201 gtgcagttct gattctgcca gtggcgggtt gcacttttct gctgcctttt gaaacaattg
     1261 atacgctgcc tctgtattgc cataacgctt gatcagtttt ccggcaatat gactcattgt
     1321 gctgtttctg gaaccctctt gaatcaattc tgtctgagca tcccattctg caaaagcatc
     1381 tttttcaaaa aattcagcaa gcgtcagatt gccttgatac cattccactt ttggattctc
     1441 cacaccaaaa aagaaatgtg cctcgtccag tgccttttca tcgaaatagg gaaactgttc
     1501 cagaacctgt tttttcaaat tctgtcgttc tgttacggac attccttttt ctgcttcaaa
     1561 atagacatga aacttaggac gtgcaattct gtttcccttg tttttcatgt ggtttctgct
     1621 ataggcaact gcgaatgcta cgtctgaaaa tgtcaatgcc agttccaaag gtgtaaccca
     1681 atcttctgga ttttcagagt ggctattatc gcaatcaaac atcagacaat cgctctctat
     1741 aaagctggca ttgcttcttt tatcatccgt gaattttgca gaaacgtggt caaattgcac
     1801 cgcagacttc aaactttcct cgtcaattac ctctacatcc ttcggatatt tgatattttt
     1861 tgcgttttca cggcaagtag cagtataaag cgtaaatttc atttctttgc ctccagttct
     1921 gcaatcagcg tatttgtctg actcataatt ccgcacactt gcttttgtat atcacgcaaa
     1981 gagtccatag taattacatc tccagactgt tcgccctctt gccccgttaa taaataatct
     2041 gtcgacacgc acaagtaatc cgccattttt aacaaaagtc tcggcgaagg agccgtttca
     2101 ccttttaggt acagagagac tgtttgcggt cgaactccaa cgtgttcagc cagttctttt
     2161 tgtgtaattc ttctgcggta cgttggatga cactccatca atttttgcag tatttgtggg
     2221 aattgataca tcactctatt tcctccagtt cttctgtaaa ataccgaatg gtcatatgcc
     2281 gccgctttgc ccatttgatt tcctgctgca tcccctccga ccgcacagaa ccaaacaccc
     2341 acagctgggc acactttgac agcagtacca aattcatgaa catcgctgtc tgacgatctt
     2401 cgcccagact gtcatccatg aactgcggaa acagcaagtg gggagcgata gggacatagt
     2461 g
//
LOCUS       DAWBGR010000267         2444 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_9786_length_2444_cov_3.872332, whole genome shotgun sequence.
ACCESSION   DAWBGR010000267 DAWBGR010000000
VERSION     DAWBGR010000267.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9786_length_2444_cov_3.872332"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          588 a          683 c          579 g          594 t
ORIGIN      
        1 tttttgtttc atctgacgaa aaatttgacg acgcatctgt cgcacaatct ttgttcggta
       61 ttgccttatg ttattccgca ctttcctgct ttgcagcttc ccgatccatg cgaatcgcct
      121 tcagcacgat cgcacattcc agacgcttct tttcgatctc acaggacagc ttgtgtgcct
      181 tgtgcacatc gccgctgaac tggtagttat tcgcgttaca gccgccgctg caatagaatc
      241 ttgcccagca gttccggcag tccggcttgc tgtagatatg ggtcttggcg aagtcggact
      301 tcatttcacg gttaaatgtg ccgtcgtcca cgttgcccat tttatagtct gcctcaccga
      361 caaactgatg acatgggtag atgtcgccgt ccggtgtgat tgccacgtac tcattgccgc
      421 agccgcagcc acgcagacgc ttgatcgcac aggggccctg atccagatcc agcatgaagt
      481 ggaagaagtt gaacttcttg ccggttgcct catagtccag aatcttctgc atcagccgtt
      541 cgtattcttc aaaaatacgg ggcaggtctg cctcggtaat tgcgtaaggt tcttccgcat
      601 cagcgataac cggttccaca gagatctggt cgaagcctgc gtccagcaga gcgaacacat
      661 catcggagaa gtccagattg tacttggtgt aagtaccgcg gacgtagtat tccttttccc
      721 cacgctggtc taccagcttc ttgtacatgg gaatgatctt atcataagaa cccttgccgt
      781 ctacgcgggg acgcatgcgg tcattgacct cttttctgcc gtcgatagac agtaccacgt
      841 tggacatttc ccgattgatg aagtcaatct tgtcatcggt gagcagcatg ccgttggtgg
      901 taatggtgaa ccggaacttc ttgtgataga ccggctcctg tgaacgggcg tattccacaa
      961 tttttttcac agtttcgaag ttcatcagcg gttcgccgcc gaagaagtcc atttccagat
     1021 tcacgcggtc gccggaattt tgcagcagga agtccaccgc ttttttaccg gtttcatagg
     1081 acatcagctt tctgccctcg ccgaaatcgc cggtagaggc gaagcagtac ttgcaacgca
     1141 gattgcagtc gtgggcgata ttcagacaca tcgccttaat gggagaagcc acagcgaagt
     1201 tggcatactt ttcataatcg tccggcgaaa agagaatgcc ttcctgatat gcctccagaa
     1261 tttccgcata gcaggaacgg acttcttccg gatcgtaaaa ccgcgacagc tttgtcacga
     1321 cttcctccgg acattccgga gcaaaaggcg gtgcaacatt atccagcatg tcataggtca
     1381 gctcgtccac cacatgcaca ccgccgctgt tcacgtccag cacaatgtta tagtcgccca
     1441 gtttatactt atgaatcata attctgtttc ctctttccgg ctgccttggg gaacatgcag
     1501 caccataaaa attcgtgtat agatgcgaaa aaagggacag aactgtccct ttcttctatc
     1561 agttgcagac ttgcgtgtga aaggttttca aaaaccctta gtgctcgcac ttctggttcg
     1621 ctactgtaca ggaagtcttg caagcagact gacaggaagc ctgacacttg ccgcagccgc
     1681 cctttgcagc agaagccttc aggtttgctg cattgatggt cttaatgtgc ttcattcgga
     1741 aaccctcccc caaaaaaagt ttaactacat tatatcacaa atccgccgga ttttcaagcg
     1801 ttctatgcaa aaacggaaaa aaatttacat cttccgtgac aattcgccgg actgtcgctt
     1861 tctgtgctga cggttactgt tatttcggcg gcttcttgtg gggagcattc acgccggaga
     1921 tgccgcccca gatactgccg ccgcagatca gcagcagccg caccggactg ccggcagtcc
     1981 cctgccagaa cagccccaca gccaacagga tcccgcacag cagcagcccg cagcacagcc
     2041 ctgtcagcca gcccttgcgg cggcggtgaa atcccgcaaa tcgtcccatg ctgtaaccgg
     2101 aacaaaacaa cagcagcccc agcagcaaag cggagatcct ttccgacaag tcagacagca
     2161 gcagtaagcc agacagggca gccagtcctg ccagaagcaa gccgattccc acggacaatg
     2221 ccgccggcag atatccgaac cggttttccc agatgctctg ccttaccggc tgccgtttgg
     2281 ttctcattgc actgcacctc cctgccatga tgtctgctga cagtgtatgc ctgcggcagc
     2341 gggattagaa cagggacagc gtacaaacag caaaaaacgg accgcaaaat tgaactgacc
     2401 ccaaaaagtt agacaaagat tcaaaagaaa atctatgcaa agcg
//
LOCUS       DAWBGR010000268         2418 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_9920_length_2418_cov_22.575116, whole genome shotgun sequence.
ACCESSION   DAWBGR010000268 DAWBGR010000000
VERSION     DAWBGR010000268.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9920_length_2418_cov_22.575116"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    381..390
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          637 a          671 c          547 g          553 t
ORIGIN      
        1 tcgcgtagtg tgatataata aaaaaccgag ggcatacctg aaacggtagg cggtcgattc
       61 ctgctcccag aagggagtga gtgcaaatga acctggacat tctcgtcaag tgcattagca
      121 tggtttgcga agtcttcaag gcatatcttg cttacttaaa cttcaagaaa cggaaataac
      181 cgcccctaaa gctgcaaact tggacagtta tttcaaataa tcaattcatg ggagcagacc
      241 gtctactggt gtgctctctt tacttttatt atacgcgatt ttctccggtt tgtcaagtct
      301 gtttttgctt gacaaactgt attttcccct gcgaaaagca agataaacta tggtttgaca
      361 tttttcgcgt agtgtgatat nnnnnnnnnn ccccccgccc gccgcacaaa cgtcggagta
      421 ccgcgggaat tccgtacatg cctgcccaga tcagagccat cagcaccggc agcccgatca
      481 gatgactgcc gaacacctgt tcaaaccaga ccagcggcga accggaggac gggcgttcca
      541 gaaagaaaaa gttggtgccg aagtggcggt tcaccagcga aaccggcaca cacgccgccg
      601 ccacacaggt gaagcaaaac ggcagccgtt tccagttggg gcgaagtatc ccgcgagcca
      661 gcagcaccgc cggcaccagc accagcagcc cgtggagcag gaagctgtgc aggctgaaaa
      721 agttccacag gggcagcgag aaccagttgg gaaacagcag tgccagacac gctcccggca
      781 gtgaaaggct gtagaccggt tccagcagca gcggacgagg ttttcgcact gcggcaaatt
      841 ccacaaaaat ggacagcccg cacagatcca gcggcagata gttgggacga aactgccccg
      901 tgtacagcag cacgccatcc ttgcagatct ccagcaaaag tgctgcccat gtcagcccgc
      961 ataacagccg atgcacagtt tccggtttcc agcggcggca ggtcagggca atcgccagca
     1021 ctgccagaac gatcccgccc agccacagca aatgggcaat gccaaacagc cgaaacccca
     1081 gcccgtccgg cacatgttcc tgaattgtcc agaagtacgt catctcctca ccctctccta
     1141 ttcagtatac cgcatttttg cgaaaaatac aagcgattct caccggaaca cacgaaaatc
     1201 aattttcatg atagcctccc ctgaaagggg agggggaccg ccgttaggcg gtggagaggt
     1261 tttttcgtaa aatcatttta tgacgttttc ggcataaatg gaaacccctc agtcagtgct
     1321 acgcactgcc agctcagcac aaggcacgcc ctacgggtgc cctttcaggg gaaccttact
     1381 tgtccaactt catgcacaat taaaatcagc atatttttaa gaattgattt ccgtatctga
     1441 ctcccttttc ccctgcatca atcccgcact gccccacaat tctcaaaatt tccggaaaat
     1501 tttcatgaaa tggctgtatc tacctgttat actaaaacaa atgagaacag ttctcattaa
     1561 ggcaacaccg cacaaagccc gcctgacggc tttgcacgca atctgcgtgc agattttccg
     1621 tcatattgcc cagaaatccc ttgccagaca actgcgttcg aggcaggatt ctgctcgaga
     1681 acaaaggcgt gtcgcactaa gattgattgc gaccatacag tctgcctgcg gaatttctgt
     1741 gcaatctgcc gaaaaatctg actacgcatc ttacgcacaa gctttattca gtgtttcctt
     1801 aattgtgcat tagagccttg tcgtatggca aggacttttg acgtagttct gccgaaaaga
     1861 cgtgtggaaa aagatactga agtcaatacc gcacaaagag cgtctggcga ctttgcacgc
     1921 catctgcttg caaattttcc gtcagagcct gtttcgtatc ttcccgcacg catcttgcgt
     1981 gcatattttc cgagatattt cgggaaaata ctcgaaaatc cacaaaggat tttctccgtt
     2041 ttccccttca tctctcaaaa aatctgacaa cgcaatctgc atacgtcaga tacgaaacag
     2101 actctcatat ttcacaaaaa tgctcgtgaa tacatcaagt attcactccg ctttttgttt
     2161 catctgacga aaaatttgac gacgcatctg tcgcacaatc tttgtgcggt attgccctaa
     2221 aaagtccggc tcccctgaaa gggcacccga agggcgtgcc ttgtgctgag ctggcagtgc
     2281 gtagcactga ctgaggggtt tccacttatg ccggaaacgc cataaaagga ttttacgaaa
     2341 aaacctctcc accgcctaac ggcggtcccc ctcgtactga gcggtcccct ctgccctgcg
     2401 ggcatctccc caccccgt
//
LOCUS       DAWBGR010000269         2387 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_10092_length_2387_cov_4.751286, whole genome shotgun sequence.
ACCESSION   DAWBGR010000269 DAWBGR010000000
VERSION     DAWBGR010000269.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10092_length_2387_cov_4.751286"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          531 a          601 c          624 g          631 t
ORIGIN      
        1 tggattccac cgacagcttc tatatcatgt actacgtggc tctccgcgga gcagggatcc
       61 accagacttg ggaggaagtt ctcgcaaagt agtccctcaa aacttgcctg acatttatac
      121 ggcattacga aaaaacagca cttttgcccg cgggtctgcg gctgcaaaag tgctgtttct
      181 atttatgaaa gtgagagcca taaaccggca caaaatgtta tgccgtcagt cctcgtcgcc
      241 ggaagaaatc ggaatcgtct tttcagcatt atctgcggaa tcgtcctgtt ccggctttgc
      301 atcgtcggca ggttcttctt ccggcatgtg cacggttgtc ggctggtctg cttcgtcgtc
      361 ctcggaatcg atagagcaaa taccatctgc acagcagcag cctgctgctt cggcagtgtt
      421 ttcggtatct tctttgtttt ccggttcgtc acagcaggag cactcgtcgt catttgtgca
      481 gccgccctcc ggcaggtcgt cacaattgca tgcggcttcc agtcgcttgt cgtgtgcctt
      541 tttcagtgcg taaatagttg cagcggaagc tgcggcagct gctgcaacca ttgcagcgat
      601 aatcgttcct ttcatatcga tacctctttt ctccgacacc atgtcctgtg caggcgtgtg
      661 cgaaagtgta cacgctgtgg ttcttctgcg ggtcggcgac agaaaacaca gacgtttcca
      721 gattacagct ttcgtttcgc tgtaatattt ctacatctat agtataccat attttcaaag
      781 aaaatacaag ccccgacatg cagatatttg cagattatcc gacaaatttt tcagaggtga
      841 ctaggaaaat atctttaggc aataccgcac aaagattgtg cagtgctgct ttatacacgc
      901 tccagtgcct gctgcaagtc ggcgagaata tcctcgatat tttccagacc tacagaaatg
      961 cggatcagtc cgccgtcaat gcctgccgct ttcagctgtt cttcggtcag ctggcggtgg
     1021 gtggctgatg cagggtgcag cacacaggtg cgaatatctg ccacgtgtac ctcgatggaa
     1081 gccagctgca atgcgtccat aaagcggaca gcagcttctc tgccgccctt gatcgagatc
     1141 gaaatgacac cgcaggtgcc gttgggcaga tacttctttg ccatttcgtg atagggatcg
     1201 tccggcaggt tggggtaggt gacagaggcg attttgtcag actgcttcag catttttgcc
     1261 actgccatag cgttttcgca gtactgcttc atacgtaccg gcagagtttc cagtcccaga
     1321 ttcagcagga acgcggagtg tgctgccgga taacagccaa agtcccgcat cagctgcatt
     1381 ctcgccttga tgatgtatgc cattttgccg aactctctgg tgtagactac accgtgatag
     1441 ctttcgtccg gctctgtgaa gtcggggaat ttgccgcttt tttcccagtc aaatgtgccg
     1501 ccgtccacaa taacgccgcc gcactggaga gcgtggccgt ccatgtactt ggtggtggag
     1561 tggatcacaa tatctgctcc gaattcaatg ggacggcaga ggatcggcgt ggcaaaggtg
     1621 ttgtccacga tcagcgggat ctggtgggca tgggcgatct tggcgaactt ttcaatgtcg
     1681 aatactgtca gtgccggatt ggcaatggtt tcgccgaaga tcagcttggt gttgggcttt
     1741 atcgcctgcg aaatggtttc ctcatctgcc agcgggtcta cccagatgca ctcgatgccg
     1801 aaccgcttca gcgttacgcc gaacaggttc acagtgccgc cgtaaatggt ggaaacggaa
     1861 acaatgctgt cgccggcact gcacagattc agcaccgaca gcagggaagc tgcctgtccg
     1921 ctggaagtga gcattgcccc gacgccgccc tccagtgcag cgatcttctg ttctactgca
     1981 tcgcaggtgg gattggcgaa acgggagtac atcggttccg tgggcatgtc aaacagttcc
     2041 gccacctttt cggtggagtc gaaacgataa gtggtgcttt gtacaatggg tactgtgccg
     2101 gggccgccgt ttttcggggt ataaccggcg tgcaggcatt tggtttccag ttttgcattc
     2161 ggattgcagg gctgcataaa aattcctcca gagtgtcggc agtacacggg caggattgcc
     2221 cgaaatcatg tactgcaagt tgtattttta taataaaaaa gatgatacta ttatagcaca
     2281 ttttattcgt tttgcataga cggaattttc aaatctaaac ccatagaaaa aatctatggc
     2341 aataccgcac aaagattgtg cgacagatgc gtcgtcaaat ttttcgt
//
LOCUS       DAWBGR010000270         2357 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_10253_length_2357_cov_2.428323, whole genome shotgun sequence.
ACCESSION   DAWBGR010000270 DAWBGR010000000
VERSION     DAWBGR010000270.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10253_length_2357_cov_2.428323"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1515..1524
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2098..2107
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          620 a          501 c          459 g          757 t
ORIGIN      
        1 gaagtctttt agagcataca acacctgcgg caagtttgta agcgtgatat tgcttgcagg
       61 cgaatgcacc cagtcgtttt tgttcctcat tcggctgatt tgctttcatt tcctgaaatt
      121 cgttgctgat ttctacatcg ttaggaaaat catcgttgat ccagcattca ataaacgcaa
      181 atagtcggtc gatcgcattc tgaaatgttt catcgtcctc acggcattcc gaagaattga
      241 tcatttcaat cagcgtttca aagttctgtt cttccggcgg attgatcgta aagatcatag
      301 cgatatatgc agtgtaaagc agcttttctg cctttaccca gaagtcatcg ccgccgtttt
      361 gctgagaact ctgcgtattc ctaatgagaa cgtccacaaa tttcagaata tccttttctc
      421 tgttcttttt ggagatgtag gcaaaggggt tgtagtgcat ggacttgcca aaatcaatag
      481 tattgaacac cttgatccga tacggctgat aggatagatt gcctttttcg tccttgtacg
      541 gtctgccacg ttccagcatc tttccgcatt cgatcagtac tgtccctttt gggtctgtca
      601 cgatgtagga actgtgcatc tgcatgagat tcggctttac gaaaaatctc gttttaccgg
      661 acccggaacc gccgatcacc agaatattct tgtttctggc gtacttcggg tgagatggct
      721 ttcccatggt caagccctct gtctgtgtga gaatgacgtt gttgtctttc tctttgaagt
      781 ccatatatgg ttcaatgtcc ttttccgtac cccatacggc agagccgtat tctgttcctt
      841 gccggaactt ctttttattg gcggctctca ttttcatcac aaatttcata gcaacgccaa
      901 cagcaattcc aataagtaaa tcaatcgggt gaaatgacgg aatgacatgt gcaaaagccg
      961 tgccgagatt gttcagggca gggagtatct tttcagaagc gttttttcca tcagctgtcc
     1021 ggaacgcaaa acagatgata ttgcctgcat aaccgaaaat gacataggca aggttattga
     1081 ggacaaattt ttttatcttt cgtgaatcca ttccaatcac cttaatgcgt catttcttta
     1141 gacttctcac ggactttttc tttcttcttt ggctcatgag aaacctgctt tgccatatct
     1201 ttgatctgtt gtcgggtaat ttcagcacgg ggattgagaa gttcctcttg ttttttgctg
     1261 gcaaattcct gaaaagccct cttgaaatct tccattttgc ccgcctgaaa gaaaacatga
     1321 taggtatcgc tttccggttc tttcttgacg gcatagtcaa tgtcatattt ctttgccacg
     1381 ctcagaaagt ccgcaatgcc tttgatctcc acgttttcca gcttgccgga atgctccgat
     1441 agctttttca tggaaactct gccttttctc tgcgactttc cggaaagaaa atcctgcatg
     1501 gcagatttca aagtnnnnnn nnnntgcatc gtcatattct gcaaccacaa taccgttttc
     1561 catatcgcct ttcagataga ccatgaagcc gtatccgctt ccgtcatcgt actcaccaca
     1621 agtgacacac gaaacggcat tagagtagtt gaatgcagtt ccgtcctgcg agataattga
     1681 taaggtcata cgctcactcc tttcccgctt actattttca gtttagcaaa atgaaaagct
     1741 taaatctctc aagtattttc aagtgttttt cagcttgtta aagttgtatg cagctttttt
     1801 aagtgatttt cagtaaagaa aaaagcttac ttttgacaac cgaaaacagt ttttatattg
     1861 aattttcaga tgatttatgg tataatacag acagtcatca tatcaaaaaa ataaagttgc
     1921 gacactgtca caacttttta agaaaggaat ttttatgagc gagttgatga aatacgcaga
     1981 gtctgctgat ctctattcca aaatatcaaa tctcataacc gcaaaaaaat atactgtttc
     2041 acgtgctgtc aattcagcca ttgtctttct ctattgggag attggcgaaa ctgtttgnnn
     2101 nnnnnnnaag gatttgaccg aacagcaatt ttcagaatgg tgcagtttta tcaagagttt
     2161 cctgataaag aaaaagttgc gacactgtca caacaattga cttggtctca ttttgtagtt
     2221 ttgcttccaa tcgaagatga attaaaaaga gatttttacg ctgctatgtg caaatcagaa
     2281 aattggagtg ttcgtgtact gagagaacgt aaaaactcta tgctttatga acgcacggct
     2341 atttccaaaa aggctga
//
LOCUS       DAWBGR010000271         2328 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_10431_length_2328_cov_3.312363, whole genome shotgun sequence.
ACCESSION   DAWBGR010000271 DAWBGR010000000
VERSION     DAWBGR010000271.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10431_length_2328_cov_3.312363"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          603 a          538 c          533 g          654 t
ORIGIN      
        1 aaaatctgac tacgcatctt acgcacaagc tttattcagt gtttccctag gaattacttt
       61 ctgcccagca cgaagtccca agtcggattc ttgcctgcac cctgcagggc aacgtagtaa
      121 aggatataat acacgtcggt ggaatctacc gaaccgtcac cgtcaatatc agcagcggcg
      181 atctggtcag cggtgagatt gctggacatg cctgcaccgc ggtatgccac gcagagcatg
      241 gcttcgaata catcgttggt gtcgagcacg ccgtcaccgg aagcgtcacc ctttgcagct
      301 gccggagccg ttgtggtagt ggttgcagtc gttgcggttg ttgctgtggt ggtgttggtg
      361 gtagttgttg tagttgtagc tgtagtttct gcggtggtgg ttgtggtttc ggtggtggta
      421 gcagctgttg tggttgatgt ggtggtctgc tctgtggtag tctgttcggt ggtggattct
      481 gtggtggcag tagtggttgt ggtggtggtt gtagtagtcg ttgttgtcgt tgtggtagtt
      541 gtcatgatag gctgaccggg aatctgttcg ttgggatccc agttgtagtg gtgttctgca
      601 ccaccaagag cacctacacc cggaatatca ttccaatctt cctcagatcc tgtataatac
      661 acatcagtca gtttatcgca gcggttaaaa caaagggtat tgatttttga gagtccctta
      721 taaagcaaaa tgctttccag gctagtgcag aaatagaacg taccgtcctt caaggtcaaa
      781 acattttccg gaatcgcaat aaatttcaga gcggtacagc cgtaaaaagc gttattttct
      841 atgctcataa cgctgttcgg aagataaatg tattccagtt tgctgcacat attgaaaata
      901 ttatcaccga taaaagtgac ggaatccgga ataacaatac ttgtcagtcc atcgcatttg
      961 gcaaaggcag aaccgcaaat gtcggttacg ttccccggaa ttgtgatctc tgcgagtgca
     1021 tcacaattcc aaaatgcacc atttccgatt ttctgtaggc tgttcggcaa tttcacatct
     1081 gttaagttgc cgcagttgga aaatgtgccg tctgccaatt cagtgaatcc ctccggaatt
     1141 tccgcggaag tcagagaaga acaaccggaa aatacttctt ttcctgtact tgtcagaccg
     1201 ttcggcaaag tcacctgcac caatccggaa caattataga atgcactggc acccatactt
     1261 gtcaaacctt ccggaaaagt tatctgttcc aatccggaac acccatagaa tgcaccttcg
     1321 ccgatcgttg tcagaccatc cggcagcgat atctgctgta atttcgaaca gtttttgaat
     1381 gcacgatact cgatggaagt gacagaatcc ggaatttcca cagaggtcaa agcagagcaa
     1441 tccgaaaaca tactttcgct gatgcttgtc aagccattgg gcaaggtcac ctgtttcagc
     1501 ttgctgcaag atgcaaatat accatttcct acagtagcaa cagaatccgg aagtgccagt
     1561 tccgttaaag cactgcactg gttaaatgcc gattcaccaa tttcggtcag attttccgaa
     1621 agggtaatgc ttgtcaattc gttacagcct gaaaaagcaa acgttccgat tgtttttaca
     1681 ccatttccca tgtccacact tctcaaatga ctacagtcat taaaagcacg gctttcgatc
     1741 gtggtcacac tatccggtaa tgttactttc agcaatttgg actttgagga caatgcacgt
     1801 tctgcaatcg ttgttaccgg ctttccctcg atctccgacg gaacgatgac tgaagtcaca
     1861 tcatcacagc ctgtgatagc cacctctgta ctgtcgttga tcagttggta agtcaacccg
     1921 tctgcggatt ccgcacttgc cacaacagac acctgcggca aagcaacctg cactgccggc
     1981 aatgctccga cgcacaaggt tgccgccgtc aaagcggcta aaatttttcc gattttcttc
     2041 aatttcattt actcctctca caatttttcc atgatacgaa cgtatatctt tccaatatgt
     2101 atgctagcat ttcctattat accgcaaaaa aaaaaaaaaa acaacttttt gaggatattc
     2161 tgtgaattat gaaatttgtg ttgctgcaaa tgcaaaaaac aaccctgtat aaagccataa
     2221 aacaggttct gtacagggtc gcttttcttt aagagaggaa tatttttgaa agggattgcc
     2281 taaggcaata ccgcacaaag agcgtctggc gactttgcac gccatctg
//
LOCUS       DAWBGR010000272         2294 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_10610_length_2294_cov_3.714158, whole genome shotgun sequence.
ACCESSION   DAWBGR010000272 DAWBGR010000000
VERSION     DAWBGR010000272.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10610_length_2294_cov_3.714158"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          530 a          557 c          657 g          550 t
ORIGIN      
        1 agagcgtctg gcgactttgc acgccatctg cgtgcagatt ttccgtcata ttgccgaaaa
       61 atctgacaac gcaccttacg cacaagcttt gtgcggtgtt gccttaggaa aaggagcagg
      121 atcatgatgg aggaaatgga aaagcattcg gaacagcagc cccatgaacc ggaaacccag
      181 ccggcagagg tgccgctgct ggaaaccata catctgtcgg acacgccggc accccgaaag
      241 cctcagcatg ccgatgacat cgccctgacc cagtgtattc tctgtctgct gctggtgctc
      301 tgcgtgtttg ggctgcactg gctgaaaccg gagtggcaga tcatgctgct ggaacagtac
      361 gccgcaaaac gggatgcacc gccggtgctc tggctggatc agctgctccg tgccgtgcag
      421 cagtggatca ccaagtgatt tgctggcact ggcgtgggac ggtgttcacc gtggacttca
      481 gcttctttgc cgtggcggca ctggtggcag tccgcggcgg agccttgtat tttctgctgc
      541 tgtttctggc gtgtctgctg catgaactgg gacatctggc aatgctgctg ttgtttcacc
      601 gccatgtgca gacagtttca ctgtgcggtg ccggagcgtt gtttgtgccg tctgctgcct
      661 gcggggcata ctggcaggac atactggttt ctcttgcagg tccggcagtg aatctgctgc
      721 tggggacagc gtttttgtgg ggagatcgcg gttcggtttc cggtatgctg catctgggac
      781 tgggcttgtt caacctgctg ccctatgcca gactggacgg cggggcagtt tgccgcagtg
      841 tgctttctgt ctgcggctgc ctgccggaac agaccgagcg gctccagaat ggcattgcag
      901 ttctgatgac cgtactgctg gggacagtct gctggcagtt ccggctgcaa aatctgcctt
      961 tctgtctgat gtgtgtttat ttgctgtgga tacagctgtg ggaatcatac gcttagggtg
     1021 acattcattg gctttgtgcg gtgttgcctt aatcccaacc aaacgcggtg aaatcgtcaa
     1081 aaaacacaaa actttgggga agtaattatc aataatcatg aaaatacaga ttttgcttgc
     1141 attttttatg gtaatctgtt acaataaatg tgagaattat gatagataca gtatacgttc
     1201 tcataggaag atatactgtt ggaacgaaag gtgagatttg caatgaatat cagtatgatc
     1261 gcacagctgg cggaagtatc tacggcaacg gtgtcccgct atctgaataa tggatatgtc
     1321 agcgaacaga agcggaagcg gattcaggcg gtgatcgaac agaccggtta tacgcccagt
     1381 tcctctgccc agacgctccg caccggcaga aacaatctga tcggtgtgat tgttccgaaa
     1441 atcagctcag aatccgtcag ccgaatggtg gacggcatca cagaggttgt caacaacgcc
     1501 ggttatcagg tgttgctggc gaacaccgga aacaacacgg aacgggaaat ggaatatctg
     1561 aaaacgttcc ggctgaacaa cgtagacggc gtgatcttta tcggaacgct gatgacgaaa
     1621 aaacacctcg ccctgatgaa atcctataag aagccgatcg ttttaatggg acagcaggaa
     1681 tccgatgttt cctgcgtata ttttgacgac tacggtgccg caaagctggc gaccggctgt
     1741 ctggttcgct gcggctgcca gaatattgtg cacctcggtg tgaccttaaa ggacaaggct
     1801 gccggacgtg ccagaagggg cggttatctg gatacgctgg aagaaaacca tattgctttt
     1861 cgggaggaac tgatcgcaga gtgtgccggt ttcaccacac aggacggcag cgacgccatg
     1921 gcggcatttc tggaaaagca gattcctttt gacggcgttt tctgtgctac agataccatt
     1981 gcattcggag caatcgacgt gctgcggcgt gcaggaatcc agattccgga acaggtgaag
     2041 ctgacagcag tcggcaacaa ccgcatgtcg gcaatctatt ctccccacct gacttctgtc
     2101 agtctgtccc accggacagg cggtctggaa gccggtgcca ttctcatgga tatgatccag
     2161 aacgacaatg atgctgttcg taccgcacag atgcagtgcc ggctgtacga acgagaaagc
     2221 actggtgcgg cggaataaat atggcaatac cgcacaaaga ttgtgcgaca gatgcgtcgt
     2281 caaatttttc gtca
//
LOCUS       DAWBGR010000273         2280 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_10689_length_2280_cov_5.440000, whole genome shotgun sequence.
ACCESSION   DAWBGR010000273 DAWBGR010000000
VERSION     DAWBGR010000273.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10689_length_2280_cov_5.440000"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          547 a          619 c          627 g          487 t
ORIGIN      
        1 taagcctccc ttgaaaaggg agggggaccg ccgctaggcg gtggagggat tctttgtggg
       61 gaatcttttg cttaatgtgt gaggctttac ggaagcaccc ctcagtcacc tgcggtgaca
      121 gctcagcaca aggcacgccc taaaggggag cctattccac ccgcatctca ccgcctcttt
      181 tccggaggct catttccaca cataccaatt cataaggagg tactgtccat gcagggaaac
      241 tatttggaac aagcactagg catgattcac tcgcggcggc tcgctgcgga acagcagaat
      301 cgggaacgca ttatggaaat tgaaagccgc attccggagg tgtcggaggt caactaccag
      361 atgttccgca ccagccgcga cctgatgcag atcattgtca agggcgaaaa cacgaaagaa
      421 cgggtggcag ccctgcggca gcagaaccta caggctcagg agatgatccg ccggcttctg
      481 aaagaacacg gctatccgga ggactatctg gaactccact acacctgcga aaaatgccat
      541 gacaccggtt attttcagca gaaatactgc acctgtctga ccgatctgat cgccaagctg
      601 tccgtcaggg aactgaacaa gagcgttcag tttgagcagt gcagcttcga gaatttcgac
      661 ctgaactatt atcggggctt ccagaccgaa aacggcggca gctgctatca ggcaatggaa
      721 aaggtctact tcttctgtca ggaatacgcc cgccagttca ccaaccactc cagcagcatt
      781 ctgatgttcg ggaagaccgg tcgtggaaaa acccacctct ctctcgccat tgccaatcgg
      841 gtgctgcaaa acggatacaa tgttctctat gattcggcga tcaactttct gcggcaggtg
      901 gaaaaggaac acttcggcag aggcggcagc aatgacgaca cgctggacac gctgctgtcc
      961 tgcgatctgc tgattctgga cgacctcggc acggagttcc acaagcagtt ctaccagtcc
     1021 accgtttaca acatcatcaa cacccgcatg aatcgggatc tccccaccat catcagcacc
     1081 aacctgaatc acaacgaaat ttccgccctc tacgacgagc gaatcacttc ccgcatttac
     1141 accacctaca cctgcctgca cttcgtggga caggatgtgc gggtgctgaa aaagtcgaga
     1201 atgcagaacg ggtgagtgta gaagaaaaaa gcctcccttg aaaagggtga ctccccacgg
     1261 ggtggggaga tgcccgcagg gcagagggga ccgctcagta cgaggggaac cgccgttagg
     1321 cggtggaggg atttcttgtg gaatctttta catgatgtaa tagatttagg ggtaacccct
     1381 cagtcagtgc tacgcactgc cagcttagca caaggcacgc cctgcgggtg ccctaaaggg
     1441 gagcctatac accaaaaggg agcctatgta caaataagcc tcccctttag gggaggggga
     1501 ccagcgaagc tggtggaggg gttctttgtg gaatcttttg cttaatgtgt gaggttttac
     1561 ggaagtaccc ctcagtcacc tgcggtgaca gctcagcaca aggcacgccc tacgggtgcc
     1621 ctaaagggga gcctgaatgt aagcggcttg ccagctcagc acaaggcacg ccctacgggt
     1681 gccctaaagg ggagcctaaa caaaaggaag gcttatattt ctgatgttat aaaaaaatcc
     1741 caaccggaaa cgccacagaa atttgacaaa ttatccaaca tatgctatac tattgttgac
     1801 tggaggaggg ggctttgaac gtgggcgaat atttcttctg tcccagatgc gggcaggctt
     1861 tgcaaaatgg tacggcctac tgtcctgcct gtgggctgcc gctgacattt ccggcgggag
     1921 atctgccgcc ctgtccgccg ctccggaaaa acagcagcaa ttgtctggca gtcacttcgc
     1981 tggtgctggg catctgttcg atattgtcct gcctgctgct cctgatcgat tcggtgggag
     2041 gcttcttctg tttcaacagc attccgctgg gggctgccgg cgtggcgttg ggcatcattg
     2101 cccgcggaga aatccgcagc cgtgaccccc gcggcagcac ggctctggcg accggcggca
     2161 tggtctgctc gatcatcggg ctttgcatcg gcgtgctgct ggcgcttctc tccatagcga
     2221 ctttggcagt actgattcgg ctgctgtttt cctacattta aggcaatacc gcacaaagag
//
LOCUS       DAWBGR010000274         2230 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_10970_length_2230_cov_7.081379, whole genome shotgun sequence.
ACCESSION   DAWBGR010000274 DAWBGR010000000
VERSION     DAWBGR010000274.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10970_length_2230_cov_7.081379"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          628 a          526 c          552 g          524 t
ORIGIN      
        1 agcatttttg tgaaatatga cgggaaattt gcaagcagat ggcgtgcaaa gtcgacagac
       61 gctctttgtg cggtattgcc cataatcata tacacaggag atttcccatt attttgtccc
      121 atacgccgtt atttcttgct cggcgataaa aactctatac agtatcatgt ggcggatagt
      181 catgcggcag cattctccac tggcagcctg tctttacgat atacagcact gcttctacca
      241 atggatcctc ttatgatatt tacttttatt tccggatgga aagaattctt tgattgcttc
      301 tcattgctcg tctgtcaaac cactttcgta atgacaccca cagaatttcg aaaatcgcag
      361 ggataaaatt tactttacaa atatataaat tgcatgacaa tcctgcaata caagaaatct
      421 tctttctgtt acaataaatg tagtttgaat tgaagtttac atcaacaaaa ggaggaattt
      481 tttgtatgaa caagcaaaaa tggctggcag gactgactgc ggtactgtgc agtgcaggtg
      541 cattatcctg tttcccggca atttcaggaa cagtatctgc ggcagagctg gtcagcaatg
      601 actttgaagt caactacggc ggctggtacg gcagtgctga tgctgtagct ttgacagcag
      661 aagacggtat cggtcacaac acgtcccgtg gtatgtctgt taccggacga accagcacgt
      721 ctgacggtgc cagtgcagaa aagggcttct atctttccgg cggcgaaacc tacacataca
      781 gcgtgtgggt gtacagtgaa actgcggagc gttttcacct gtccctttcc tgtgcagatc
      841 tggacactgc acaggaaacg gaaatcgaac tgacggcaaa acagaccaga gccggcaaat
      901 ggacagaact ttctgcttct tatcgtgcac cggaaaacag cggcgaattc cggctgacca
      961 ttaccacaga cagcaccaac gattttgtgt ttgatgatgt caccgtaaca ggaaagtcgg
     1021 acagcagtga ggtttctgcg gcggctgcgg aaaagggact gaaagacgag ttcgccgatt
     1081 attttcgggt gggcaatatt ctcaacggca gtactgtaaa gaactccacc attactgcca
     1141 gcgtgctgaa ggactataac agcatcgagt gcgagaacga aaccaagcca gatgcaacgc
     1201 tggtgcagtc tcagtgcagc gaaacgaata tcggtgtttc tctgaacaat gctgccagca
     1261 tcatggactt ctgcgtcaat aataatattg ccatgcgtgg acacacactg gtttggcata
     1321 gccagacacc gctgtggttt ttcaaggaga acttcaatgc cagcggaaac tgggtttcct
     1381 cggcagtcat ggatcagcgt atggaaagct acatcaaaaa tatgtttgcg gcaatcaaga
     1441 cccagtatcc ggatctgaat ctctatgcct acgacgtggc gaatgagtgt atcagcgacg
     1501 acagcaaccg taccgccaac aatggcggca ccagagagcc gggcgagaac atcagcggac
     1561 aatctccgtg ggtacaggtc tacggcagta atgcctttgt ggaaaaagcc tttacctatg
     1621 cacggaaata tgcaccggaa agctgtgccc tttactataa cgattacaat gagtattggg
     1681 atcacaaacg ggatgctatt tatagtatgt gcaagtccct ctatgaaaaa ggcttgctgg
     1741 acggcattgg catgcagtcc cacatcaacg cagattatga cggattctcc ggcgtgtcgg
     1801 catacaccac ggcaatgaaa aagttcctgt ccattggctg cgatttgcag atcacagaat
     1861 tggacatcac tatggaaaac ggcaagtata ctctccagca gcaggcggat aagtacaagg
     1921 cgatctttca ggctgctatg gactggaaca aaaatcccag ctcagacggc agagtgaccg
     1981 ccgtgtgcat ctgggggccg aacgatgcca acacatggat caaaacggag aacaccccgc
     2041 tgttgtacga taccaatcat cagccgaagc tggcatatac cacactgacc tctatgatcc
     2101 cccagtcgga gtggggcgac ggcagcaatc ccggcggcaa tgacaaaccc attgaaccga
     2161 atgaatacgg ctggtatttc caaagcacat ttgagggcga tacagacagc tgggaaggca
     2221 gaggcagtgc
//
LOCUS       DAWBGR010000275         2217 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_11038_length_2217_cov_4.370490, whole genome shotgun sequence.
ACCESSION   DAWBGR010000275 DAWBGR010000000
VERSION     DAWBGR010000275.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11038_length_2217_cov_4.370490"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          409 a          675 c          625 g          508 t
ORIGIN      
        1 ggcaaccacc ggcgttggca agccacaggc ggacagcgag tgtaacgagc gaattccgcc
       61 ggacggtttt gacatggcag ccccagaagt tcccgcggag cgatgtggac atcgccccct
      121 acgcactccc aaaaacaaag ctagccacag tctgcccgca aatttcctgt ccggctattc
      181 cgctatctct gctccggctt cacatacaat gtgtgctgat tggtgaagat caccatgccc
      241 ggcttggctc cggcaggctt tttcacatac cgcacggcac agtaatccac cggcacctgt
      301 gccgaatccc gtgccttgct gtgatatgcc gccagctgtg ccgcttcttc cagcacctgc
      361 tgcgaaggct ctctgccgcc ggtttccaga atcacgtgtg aaccggggat attctgggtg
      421 tgcagccaga tgtcgttctt gtttgccgtt ttcaaggtca gctggtcgtt ctggcgattg
      481 ttccgcccca ccagaatccg tgtgccgtcc gaagcggtaa actccatcgg cggcagagat
      541 ttcggcggct tgccctttcg ccgcagcagc cggagatagc cctgttctgc cagttccgcc
      601 cgcagctgtt ccagatcatt ttccgtttcc gcacggctca gagcgtccag cacgctgtcc
      661 agatacgtca gttcttccct gcccttggcg atctgctccg tgagaatttt ctccgccgtg
      721 caggctttct tatacgcctg atagtacttc tgggcgttct ggggcggtgt caggcgagcg
      781 tccagtgcaa tgtgcaccgt cgggcagtcc ggctcgtaga agtcctccag aaccgcctct
      841 gtatcgccct tgtgcagccg gtagaggttg gcggagatca gatccgcttt ccggcggtct
      901 gtgtcctttt ccccgcaggc ttccagttct tcagactggg aaacgattcg ccgctggatt
      961 cgctccgttg cgtggagcag caggtggaac aagtcgttcg cccgctgttt cagccgtgcc
     1021 atgtggtcgc ggctggcgaa gaattcgtcc agcagagcac aggcagaggg catgactttt
     1081 gtcaccatca gcgtgccgta ctggtgcagc ggcaggaacg agaaatcctt cggctgtccc
     1141 tcgcgtgtgc tgacaatggt gtagactgcc tcgccctgtt ccagaatttc ccgcgttctg
     1201 ccaatggtgt aacagagccg gtctttctgg tcatcggtaa tggattccgc ccgacagggc
     1261 tgtccctttc cggtatagta tgcccattcc cgcaccagaa ccggcgaaac gccctcaaaa
     1321 gtttgcagca gagccttgga aagttctccc gttttggtgg acagcaccgc cagcatctgt
     1381 tccggtgccg cctgtaaaaa gttcagccgt tcgtcccgcg gcggcatctc atagggcagc
     1441 cccggcagca ccagccgctt gctggacatg gtggcatcga cacgtttcat gctgtcgatg
     1501 acctttccgg tttcgtcaat gaggatcaga ttggaatacc gccccatgat ctcgatcgcg
     1561 agagtcagct gcacaatatc acccagttcg ttgacgcagg agaagtcgaa gtacagcacc
     1621 cgttccagtc cgtcctgccg aattgccagc agcttgccgt ttcccagccg tttccgcagg
     1681 agcatgcaga acataggcgg agccttgggg ttttccacag ccacctgtgt gaggtgcacc
     1741 cttgcacggt ctgccgcaca ggaaaacagc acctttgccc cgccggtacg cccgcggaaa
     1801 gcgacaagca gttcttcccg tgtcggctgg tggatttttt caatacggga atccagcaac
     1861 ggcagcagtt cctgccgcac cgcgtgtaaa tatgcaccat ctaatgccat gtttgattcg
     1921 tcctttctta cggcgggcgg cgagtcgccg ctgaccttac gccccgattt caagtgtgaa
     1981 acgggtagtg ttacgtcggc agcagccgcc tgtttgcccc gccgggggat tgcggcgagt
     2041 cgccgctgac cttacgcccc aatttcaagt gtgaaacggg tagtgttacg gcgggcaagc
     2101 cgcctgtttg ccccgccggg ggatttttgt tggtaaaaaa ttgtagcaag aactacagaa
     2161 attagcctcc cctgaaaggg gtgactcccc acggggtggg gagatgcccg cagggca
//
LOCUS       DAWBGR010000276         2194 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_11185_length_2194_cov_4.822814, whole genome shotgun sequence.
ACCESSION   DAWBGR010000276 DAWBGR010000000
VERSION     DAWBGR010000276.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11185_length_2194_cov_4.822814"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          484 a          569 c          617 g          524 t
ORIGIN      
        1 tgcaagcaga tggcgtgcaa agtcgccaga cgctctttgt gcggtattgc ctcaatgcta
       61 agaaaacacc gcacaaagcc gtcagacggg ctttatgcgg tgttttctta aaagtctgct
      121 ctctctgctg caacgcagtc attccgtaaa cgtaacagtg gcatctacaa cggtcttatc
      181 gccgctgatc aggatctggt tccggctgct gtcattcaga gagccggcgg cgtaagagta
      241 accaacatta tagtcgctgt ttttgccgtg gagattcagc tgtacatcgc cgtagtccat
      301 ggcaatggtg ttcttgccgt acagggcggt atcaatggtg ctgaaatccc cgaattccag
      361 atcaaagctg ctgttccaga gggtactggt ttcgcagtgc acatcaccgt cgaaaatggt
      421 gaacgcactg aactgtgcct gatcggtttc ggtaaacctc tgcaatttta agctgccgta
      481 gtccagttcc atttcgctgc tgccgcagga cacgtctgtc agccgcacat caccggcatc
      541 gtttttcacc ttacagaggt ttccgatctc tgtctgcttg acagtgaggt caccgtagtc
      601 cagctccagg gaagctgtgc cgctgacaat gctgtctgac agcaacagat cgccggcatc
      661 attatccgca gtcaggtttg ccgcagtgac atttttcacc tgacaatcgc cgtagtccag
      721 atcgacagac agggtgctga catgaatgcc ggaaatggta cagtcgccgg catggttggc
      781 aatggcaacg gctctgtatt cggcgggcag ggtgacggtg atctttgcgg caggggcatg
      841 gaacgggtga aatccgaaat gggtgtagct gtaccacggt tcggaatcgg aattcagctg
      901 gatttgcagc gtgtcgtctt ctacggcaac atgatagctg ttctgcggca cattttcagc
      961 agtaatggaa aatttgctgc cgttttgaac tgtcacgtcg gcgttgtcaa tgtccatgag
     1021 cacagaggta aatgtttccg agggtgtgtc ggtctggtcg atgaggtgca ggttttcccg
     1081 atagcggttc tgatccagtg ctccggcaca aaccgctgca ccacagcaga tcgttccggc
     1141 acaaaggcag ctgacaccgg caatgatcca aggtttcatt ttcatttttt taattgctcc
     1201 tttcagtggt ctgcaatgcc tgtttcagct gggcaaactg ttctgccggc agcgtcagcg
     1261 tgacatcgga atcggaggga aacaagggca gccgcgaacg aacggaaagt gtggtgcccc
     1321 gttgtttggt gatacagcgg tctgcggttc gaatggacgt ggattttccg gaacggatcg
     1381 aaaggtgttc cagctgaatg gtttccacgg ctgccggtgc agcggtctga gatcttgccc
     1441 acggcgaaaa ggaacccgtg gcaccccagc ccagcagcac cagcccatag gcggcagcga
     1501 cgacaagaat tcgttttttc actgggtttc gtcccctttc agcacaaagt gcagcatttt
     1561 tttcgtggta aatatggtga accggacaat gcccttgcac gccagccaga tgggataggc
     1621 aacgagaatg ccgacaccgc cgctgcccag tccgaagccc agcagcatca gagcggtggg
     1681 aacgtcctga aatacggtaa ttacaaagct gaacagtccg cccagcagag tggctcccag
     1741 cgaaacggga atgcatatca gcacgatcag ggcggtgagc agcaggctgt accatgtgac
     1801 cagcagcggc agccagatgt aaaaggtgca tgccagaacg atcagctttg ccgtgcggtt
     1861 cgagtgtttt atgacagcgg gctggggaga aatgccgttc tcccgcagga tctgctgtgc
     1921 cagtgcttcc ggcgtgccca gtgcggcacg ttcttcttcg ctggcatctt ccagaaattc
     1981 cagataatag cggatcgttt cctgccgttc cgctgccggc atgagagaaa gtgctcgttc
     2041 cagacgggca cagtatactt gtggtgtcat aggaatatcg ctcctttttg atgattgctt
     2101 tttggatcat gaggggttgc cctaacgaag aaactttcat gaagcaaccc ctccaccgcc
     2161 taacggcggt ccccctcccc tttcagggga ggct
//
LOCUS       DAWBGR010000277         2191 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_11207_length_2191_cov_3.771067, whole genome shotgun sequence.
ACCESSION   DAWBGR010000277 DAWBGR010000000
VERSION     DAWBGR010000277.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11207_length_2191_cov_3.771067"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          573 a          539 c          612 g          467 t
ORIGIN      
        1 gcaagcagat tgcgtgcaaa gccgtcaggc gggctttatg cagtgtttcc taaaggcaac
       61 actatgctaa acctgcaagc gggagttgtg cggtattgcc atagaggaca tgctatgcaa
      121 aaggggagga ggctttatgc atatgaatat caatcaagac ttacaggaac agctgtttcg
      181 ccagcgggaa gaacagatct gccacttgga ttacaatcag gagtttcgct attacaatgc
      241 cgttgcttca ggggatatgg aaagcgtcaa ggaatacctg atgccggaaa atgatgacgc
      301 atacagcggc agcgagtacg gaaagctttc ccaaaacagc ctgtggaatg cccgctatca
      361 ctttgtggta gcggtggcac tgatcgcacg gatctgcgtg gagcaccaca tggaacggga
      421 gatcgcctac acgcttagcg atgtgttcgt tcaaaaaatt gatgactgca agaccgttgc
      481 ggaaattgcc gccctgcata atattatggt gcgggacttc accaagagaa tgcagctgct
      541 gcaaaaagcg gcatactcca tgcacgtggc tcgggcaatc gactatatca ccattcacct
      601 gcacgaaaag cttcagaccg gcaacatcgc agatgcgatc tcgatcaacc gcggttatct
      661 ttccacattg ttccggcagg aaaccggaaa gtcacttcac gactatatcc ttgcggaaaa
      721 aatccatgcc gcagctcaga tgatcaccac ttcggaaatg tcattctctg aaatttctca
      781 atactacagc ttttcttccc agagccactt tatccaatgc ttccgaaagg aactcggcat
      841 gacaccgctg gaatatcgaa aacggtatta tcgttccagc gaagtgagtg aaggaaacac
      901 tgaataaagc ttgtgcggtg ctgccataac tatacgaata cgaagaaaac aggagagaga
      961 atatgagtga aaaatgggta acggcatggg gcaatgccat ttcagttgct gaacgccgtc
     1021 cggaaaatta tgcgaaaaat ctgacactgc ggtatccggc aaaaatgatg ctggacggca
     1081 gtgcactgcg gatcacgttg gacaacttct gcggaaacga accggtgcat ctgactggtg
     1141 ccaccgttgc cgtgtcggac ggtgcagacg gcattctgcc ggaaacactg gttccgctga
     1201 ctttttacgg aaatgctggc gtgacgattc cggcaggaga acggctgcaa agtgatgcgg
     1261 cagcgtttcc ggtggaaaag ggaacgacca ttgcagtgag tctgtacttt gccgaattca
     1321 cggaaatgcg ttctggtgta gtgattaccg gaccgctgtc cggcggctat tttgcagtgg
     1381 gtgaccagac tgccaacgcg gtgctggata cggacacttc gaaaaagacc cacacggtgt
     1441 atttcctcag tgacattgac gtgctgactg ccacagaaaa tcgtaccctg atctgcttcg
     1501 gggattcgat cacagcacag gcgtggccgg attacctgat ggaacgcact ctgcaatgcg
     1561 gggacggtac cactgcggtg attcggaaag cagcaagcgg aacgcgaatc ctgcggcagt
     1621 atgacaacat cacctatgat tcctacgggc tgaaagggaa aacccgtttc cccagagaaa
     1681 ttcaggttgc cggtgcagac acggtgctga tccagcacgg catcaacgat attattcatc
     1741 cggtgggaac agatgtcaac cggttccgtc cgtggagcga cctgcccacc gcggcagaaa
     1801 tgatcgaggg gctgcggttc tatatccgta ctgctcgtgc cggcggactg cgggtgtaca
     1861 tgggaacgct gctgccgatc gagggctggc gaacctatgc ggactttcgg gaaaagctgc
     1921 ggagcgaagt caaccagtgg atccggacaa ccgacgaaat tgacggctgc gtggactttg
     1981 accgtgcagt ttgcgatccg gagcacccta ctgcttttgc agcaggctat gacagcggcg
     2041 atcatctgca ccccagcctg accgcctatg ccagaatggc agaggaagtg ccggaagcac
     2101 tgctgcgaaa cgaggagagt cactgacgtg cgaagtcctt atcaaaagac gatgtacgcc
     2161 tgttttatcg gctatattgt gcaggcagtg g
//
LOCUS       DAWBGR010000278         2187 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_11238_length_2187_cov_4.192777, whole genome shotgun sequence.
ACCESSION   DAWBGR010000278 DAWBGR010000000
VERSION     DAWBGR010000278.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11238_length_2187_cov_4.192777"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    562..571
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          529 a          508 c          615 g          525 t
ORIGIN      
        1 gtccttgata aggccaacgc aggacttgca gactttcttg tcgttgaagt cgatcaagtc
       61 atctgtgcgt ccgcaaagag cacagctgca ctgtgccttc tggataatga ttttttcacc
      121 ttcggtgtag atctgcaggt agtcgttgca ctcgatctgc agctgccgac gcagttcttt
      181 cggcaggaca aaacgtccga ggctgtctac acgacgcata atgccagagt ctttcatttt
      241 acacgatctt cctttctgcc tgtttgcttt taccagaagt aaacagattc ctttgcctgt
      301 ttttgaaaag aaatacacag tttctgccgg atccctgcgg gcagacctgt cggttttacc
      361 gctgaaaaaa cagcaattcc gcagtgttac agagatctaa agtgcatttt ctatggaaaa
      421 caggtcactt ttagaccgga ggaatgtatt ccgatctaca agtgctatta tacaacattt
      481 tccgaaatct gtcaatctat aaaatgactt ttttctccct atttcggaaa cttatcattc
      541 cataattttt tcttatatta annnnnnnnn naaaagcact tgacagaacc ggaatgatgt
      601 gctataataa tatacgttgc ttgtagcatc atgagatatt agctcagttg gcagagcata
      661 cgccttttaa gcgtagggtc gagggttcaa atccctcata tctcaccaag aagtccgtat
      721 catggtttgt gatacggatt ttttgctgta tgcggcaatc tcataaaaat ccgcctgtcg
      781 gatttttatg aggttgtcct aaggcaatac cgcacaaagc ccgcctgacg gctttgtgcg
      841 gtattgccct gaagccggtt ccgcgggcag ccggcaggag agaaaggcgg cgtagcggcg
      901 tggaactgga aaaacaaatc cggctggctg gtcgggtaga ttctgtcatt caaaaaggca
      961 tgcaggaggg aaagatcacc ggtgcctgcg taggcgtata ccagaatcag gaagaatatt
     1021 tgtgtaaggc atacggtctg gcagatcggg aacaggaaac gcccatgcgg acggacaccc
     1081 tgttccgcat cttctccatg accaagccgg tgacggcggt ggcgaccatg cagctttggg
     1141 agtccggcaa gctggaactg agcgatccgg tttcgtggta tattcctgcc tttcgggaga
     1201 agctggtaga tgtgcacggc gaactggtgc cggcgaagcg ggagatctgc attcaggatt
     1261 tgctgaacat gacctccggc attccctatc cggactgctg gagcgaatcc cagaagaaaa
     1321 tgggagcgtt ttatggcgag atcgaccgcc ggaacgccac tgaccaccct gtggatacgc
     1381 aggaatttgc acggcgtgtg ggggaagaag tgccgctgct gtttcagccg ggagagaagt
     1441 ggtcttatgg agcttcggcg gatattctgg gggcagtgct ggaaaaggcg gcagacatgc
     1501 cattggacga gctgtaccgg aagcagatct tccagccgct gggcatgaca gatacagatt
     1561 tctacgtgcc ggcggaaaag cgttcccgtc tggcacagct gtatcagtat cactggggcg
     1621 aagatttctg cgggctggtg atagagccgg atccccatct gggtatgacg gactaccgga
     1681 agaagccggc gtttttctcc ggcggggcag ggctgatctc taccatgcag gactatgccc
     1741 ggtttgccaa tctgctggcg ggaaaagggc tgcatcggga aagcggcgtg cgtctgatcg
     1801 gcgaaaacac atggcggtat ctcaccacgc cgcagttgag cgatgcccag aaaacgggaa
     1861 tcgactggga gcagctgaaa ggctatacct atggcaacct gctgcgggtg ctggaagatc
     1921 cgctggtgtg tatgaccaat gtgccggctg gggaattcgg ctgggacggc tggacaggac
     1981 cgtatttctg tgtgtccccg caggacggca ctgtgctgtt gtatctgatt caggtctgcg
     2041 gcggcagcac cagtgatatt gtgcggaaac tgcgggcgat cacattcggg tgcttgtgat
     2101 gataaagtga aaaacagact tgacaaatca gcaaaaatcg cgtataataa aagtaaagag
     2161 agcacaccag tagacggtct gctccca
//
LOCUS       DAWBGR010000279         2181 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_11274_length_2181_cov_4.964252, whole genome shotgun sequence.
ACCESSION   DAWBGR010000279 DAWBGR010000000
VERSION     DAWBGR010000279.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11274_length_2181_cov_4.964252"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          603 a          574 c          584 g          420 t
ORIGIN      
        1 ccgaaaaatc tgactacgca tcttacgcac aagctttatt cagtgtttcc taaagctttg
       61 cggcggattc cagggaaaag aggaaatgta atttggcaaa catattttgt gtggaagacg
      121 acaccggcat tcgggaactc atcacctgtg ctctccagtc cggcggctat actgcgaagg
      181 gcttccctga cggaaaaagc tttttccagc gtgtacagga agaacagccg gcattgatcc
      241 tgctggacat catgctaccg gacgaggacg gcatctcgat tctgaaacga ctgaaatccg
      301 atgcggcaac ggcggaaatt cctgtcatca tgctttcggc gaagtcctcg gagatcgaca
      361 aggtcaccgg actggaaaac ggtgcagacg attatatcac caagccattc ggcatcatgg
      421 aactgctgtc ccgcgtcaag gcggtgctgc ggcggagcca tgtgcaaaag ccggaacagc
      481 agcaggtgct ctctgcccat gacctgacca ttgacctgaa ccagcgtacc gtggtctatc
      541 tggaaacgga gatcgttctc acctataagg agttcgaact gctgtcctat ctggtacgca
      601 accgcggctc tgccatttcc cgcgaccggc tgctggaaca ggtttggggc ttctcctatg
      661 agggcgaaac cagaaccgta gacgctcatg tcaagacgct gcggcaaaag ctggaggctg
      721 ccggctgtac caatctcatt cacacagtac ggggttacgg ctataagatc ggagggtaat
      781 tatggaatgg ctcggttggg catttgctgc ggcaacggct cttttgctga caatttttat
      841 ttttctgtat ctgcaccaga aacagcaggc acagcatcag cacaagaaaa gtcaaaaggc
      901 tcttggaaag ctggaaaacg aaatgaaccg catgcagaaa gagctgcacc acgaacggga
      961 catcatcggg ctgattcagg agcacatggt agagggcatt ctgattctgg acgattccga
     1021 caagatccag cttgccaacc gcacggcaac ggcactgctg aacatcagca aggaaagctg
     1081 ggacaacaaa tccattttca ttctgacaaa caatcaggac tttctggacg cactgcgaaa
     1141 atccaaaaca gcacctcagc atgaactgct gccggaagac agtgaactgc acaagggctg
     1201 ctctgtgcgg cagctgctga agatcgacgg caagtatatt cgggcgtaca tcacccgcgt
     1261 ggaaatgggc ggcattttcg gcacgatcgt gctgctggtc gatgtaacct acaacgtcaa
     1321 ggcggaaaaa atgcggcagg aattcaccgc aaacgtttcc cacgagctga aaacaccgct
     1381 gacgaccatt aagggattcg gcgaaatgtt cggcagcggc atgatcacca aagaggaaga
     1441 cgttgccaag tacggtgcca tgatcgaacg ggaaagcgaa cgcctgctgt ttctcatcaa
     1501 cgacattatc cgcctgtcgg agattgagga acacacggaa atgctgaaca caccggtggc
     1561 actggaagct gcggcaaaga acgccctgca aattctggag ccgcagattc agcggaacaa
     1621 aattcacgtc cggctggagg gcgactgtct gctgctccac agcaacgagg gctacatacg
     1681 ggaattgttc atcaacctca ttgacaacgc gatcaagtac aacaatccgg agggcaccgt
     1741 gacgatcacc atacagcacc tcggcacaca ggcgaaaatc gtcattgcag acaacggcat
     1801 cggcattccc ctacaggcac agagccggat ctttgaacgg ttctatcggg tggacaaaag
     1861 ccgttcgaaa cagcgtggcg gcacaggact ggggctgtct atcgtcaagc acatcgtaga
     1921 ctgccacaac ggcaccattt ctctccgcag cgaactggga cacggtacgg aaattacgat
     1981 tctgctgccg gcaaagtaac gataaccccg cacaaggttt gtgcggggtt gtccaaaaga
     2041 aaggacaaac aacatgagaa acgtcatgca ggaacaagat gtgaccgact ggaaagaacg
     2101 tcttgctgcc tatacgcccg aaaccgaaca ggaacgccgc gaccggaacg agatactcct
     2161 tgcagcggaa cagtacggca c
//
LOCUS       DAWBGR010000280         2134 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_11596_length_2134_cov_5.392015, whole genome shotgun sequence.
ACCESSION   DAWBGR010000280 DAWBGR010000000
VERSION     DAWBGR010000280.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11596_length_2134_cov_5.392015"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          467 a          601 c          533 g          533 t
ORIGIN      
        1 gtcgccagac gctctttgtg cggtattgcc taaggaaaca ctatttccac acttctcccg
       61 cgttttccac agcactttca acaacttgtg gaaaacaaaa accgtggttt tttgcgtatc
      121 ctttcgaatt tatgacaaac attgcacaat ttacggggat ccggcaccaa caccctgtgc
      181 aaaactcgcc agatcgtaac cggtttgtaa ctggttttcc cctgtgttgt ggaaaactgt
      241 gttgaaaagt tggggaaata ccggtgaaag atcctgtcga ttctgtaaaa ttcgcggaat
      301 ttcgcagatt ttacagccgt ggaaatgtgg aaagagaata tttcgcggaa tgtgggaaat
      361 cctaattctg cgaggcattg aagcacattc ctgcaaaatg gaatgcattt cttcttcgcg
      421 actttcaaaa acgaggtgag atccatgaaa aactatcgat ttctggcact gcctatggca
      481 atgctcctga tcggcagtgc attcaccggc tgcggcagta agaatgacag ctccgccggt
      541 gattccacaa gcacttctgc cacagatacc agccgcgaag acggcggcgt gatgagcgat
      601 gtagaggacg gcgtgcggga tattgtcacc gatgcaggcg atgcggtacg tgatgccggc
      661 gatgcagcgg gcgacgccgt caaagatgca ggggacgctg ccggcgatct gatgaacgac
      721 gacgattcca agacagattc cacggacgac agcagccgct aacccagagc gtgtgcacat
      781 gctccggaca aaacaagagc ctcatttctg caaaagctgc ggaaatgagg ctcttttcat
      841 gcattttcag ggaatgctct gcaattactg ctgttcaaag cgacgcttca gcttttcaaa
      901 gaaactctgc cgcttcgcct gatttttttc ttccagactg tcgtcgaact tttgcagcag
      961 ttccttctgc tggcgtgtca gctttctcgg cacttctacc actacggtga catactggtc
     1021 accataggat tctctgccca gatacttgac accctcgccg cgtagacgga acttggtgcc
     1081 gctctgtgta ccctccggaa tgtcatattc caccttgccc ttaatggtag gcacagtcac
     1141 cttatcgccc agcactgcct gtgcgtaagt gatcgggatc tcacagtaga tgtccacgcc
     1201 tctgcgggta aacagatcgt gcggacgtac atttacggaa acattcaggt cgccgctggg
     1261 accgccgttc agtccggcat caccctgtcc gccgacacgc agcacctgtc cgttatcgat
     1321 accagccgga atcttcagtt caatatcgct ggtagtccgg cagcgtccgc tgccgcggca
     1381 cttgctgcac ggattggtga cgatcttgcc cttaccgccg cagcgggagc aaactcttgt
     1441 ggaagatacc gtgccgaacg gtgtccgctg gttgacctgc acggaaccac ggccgtggca
     1501 ctcggtacaa gtgctggttg tggtattggc atctgcacca gtaccgttac actggtcgca
     1561 cttcaccatg tgagtgatcc gcatcttctt cttgacaccc ttgcaggctt ccatgaagtc
     1621 gatcgtcacg ttgctgttta catcgccgcc acgccgcggt gcattggcac tggacgcagt
     1681 gcttctggaa ccgccgccga agccgccgaa gaaactttcg aaaatgtcgc tgacatcact
     1741 gaaaccgcca aatccaccga agccgccggc tcctgcaccg gctccgccgc cgtaattcgg
     1801 gtctacgccg gcatgaccga actggtcgta ccgtgcacgt ttctgggagt cagacagcac
     1861 ttcgtacgct tcgttgactt ccttgaattt ttctgctgct tctttatcgt ccggatgcag
     1921 gtcagggtga tatttccgtg ccatggtctt gaacgctttt ttgatctcgt cctcggaagc
     1981 acccttttgc agccccagca cctcgtaata atcccgtttt tctgccatgt ctgctggctc
     2041 ctttcccttg aacagacccg atcggaatcc ggctgttcct ctatggcaat accgcacaaa
     2101 gattgtgcga cagatgcgtc gtcaaatttt tcgt
//
LOCUS       DAWBGR010000281         2086 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_11954_length_2086_cov_2.895126, whole genome shotgun sequence.
ACCESSION   DAWBGR010000281 DAWBGR010000000
VERSION     DAWBGR010000281.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11954_length_2086_cov_2.895126"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1753..1762
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          621 a          475 c          420 g          560 t
ORIGIN      
        1 cgtcatattg cccagaaatc ccttgccata cggcagtatg cctgcggaat ttctaagcaa
       61 tctgccaaaa aatctgacaa cgcaccttac gcacaagctt tgtgcggtgt tgccctaaga
      121 acttgttccg aatttctggt tctattatag cagtttagtt tttcagaagt gatataactg
      181 caaaattttt ttagagcaat attgctgttt ctatcaagtt gcacagtttt tacagcatcg
      241 catttgtcta attttcctct ggctttttgt cgcaaaatat gctataatat gattatgcta
      301 tatgataaaa cgataaacgc acctcaacaa cgctgcggca gtgcggcttt gcttacaaat
      361 tccaatggag ggaatatgat ggaaaaccat tcgaacaaca aaaaaacagt atataccctg
      421 aacgaatatg tttcttacgg aacatccgga gtatgcaaga tcgaatctct ggaacagcgt
      481 tccttcgatg gggaacatga ggaatcctat tacaagctcc gtccgctgga caactccaat
      541 tctacatttt atgtacccat agaccgtgct gcggaacggc tgcgtccact gctcaccaag
      601 gagcagatct accagctgat cgacgaaatg gcagaaggaa aactggaaga aacagaatgg
      661 tgcagcaaca gtcgggaacg acgcggcatt tttcagaaca ttctgcacgg cgacgactat
      721 cgggaaatga ttcaaatgat gcgttctctg tatcaccagc aggaacgcaa acggaaaacc
      781 ggtcaccggc tatcctccgc agatgtggca gccatgcatg cggcggaaaa ccgcatgtat
      841 caggaattcg gcgttgttct gaacattcgt ccggaacagg tacataactt tatcctgaaa
      901 cgcatggagg gcagcactgc tcccgcaaaa taagaacaac aaaaaatcct tgcagtattt
      961 cactccgaga tactgcaagg atttttttgt ttagggcaat accgcacaaa gtcgccagac
     1021 gctctttgtg cggtattgtc tatagtacta cactatcggc attgtgtgaa actctcataa
     1081 aacatttcgc gaaaacgggt gcgacattgt aaattcaaaa tcaatttttc ggagaatctg
     1141 acagtttggg ttaatcctgc gtaatatgga aaatgacaga aaaaaagcag cctgccttcc
     1201 caaaacatct cgcaattgcg aaattgtttt gaaaaggcag actgctgata acgattcaca
     1261 aaaagtttga gcagacctac aaaaaatcat atttttgcaa agtatccgat gacatctttt
     1321 cgggtaataa ttcccacaaa cacgtttcga tcatcgatca ccggcacaaa attctgatcc
     1381 atgacacgct gcaaaagatc gtccatagtt gcagaaactt tcaccggcgg attaaaatcc
     1441 gggcgaagca aatccgaaac agactgatgt tccagactgc gaatatcctg ctgcaacgct
     1501 gcattgacag gattttttcc gagcacgttc cagagaaaat ccccttctgt aatcgtacca
     1561 caatagattc cgtcatgatc aattacagga atcgcggtat acttatgata gtacattttt
     1621 tccagaccct gccgcacaga atcatcggta tacaaatagg cagtatcaca tttcggcttg
     1681 atcaaaaaga taatattcat cacagtaccc tttcttccaa aaatataacg aaaaaaggtt
     1741 cgttccgtaa gannnnnnnn nngtatattt ctattatacc atattgtcta acaaaagggg
     1801 agcatttcac ttttccaatc tgccctgatt tttttctttg catcggtcgg ctgccatgtt
     1861 tacagcagtt gtgccgagtc catttctgct gggactcggt tcgaatccct ctacaccgaa
     1921 aaaaatgccc aaaaatatct acggtttttc acctgctttt ttcgtcgttt gggcatgaaa
     1981 aaagcccgat atttcgggct ttttcgacca tatatctttt ttatggtcac aatatggtgg
     2041 gccatcgggg actcgaaccc aggaccgacc ggttatgagc cggtag
//
LOCUS       DAWBGR010000282         2072 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_12059_length_2072_cov_4.011403, whole genome shotgun sequence.
ACCESSION   DAWBGR010000282 DAWBGR010000000
VERSION     DAWBGR010000282.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12059_length_2072_cov_4.011403"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1543..1552
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          568 a          513 c          563 g          418 t
ORIGIN      
        1 aacccttacc ggcttgtaag gtgttacccg actaattata ttgtaacagg agtgaaaccg
       61 aatgaaaacc ctcatagatg tccttacagc actcagcgat ttcgtaaaga tcgcatcaga
      121 gtgggcagaa agtgcttcca aggcggaagt ggagacgttc acacagatct gtccgcaaaa
      181 ggaagaaacg gtcaaaaaag cagtggaaaa gtcggtcaca ctggaagagg tccgcagcgt
      241 tctggcaaat ctgtcccgca gcggacaaaa ggaaacggtg ctgaaactgc tgcaaaagta
      301 cggcggcagc cgattgtctg aagttccgcc agaacagtac gctgcactgt tcgcagatgc
      361 acaggaggca gcccatgccg aataaacacg cagtactttc tgcttcctcc agtgctcgtt
      421 ggctggcgtg tccgccgtcc gcacagcttt gtgctgccct gccggatatc gtgacagact
      481 acgccctgga aggcacgtgt gcccacgagt tggcagagta caaagtgcaa aagctgcttg
      541 gcaatccggc atctaatccc acggagaact tagacttcta cgatgcagaa atggaagact
      601 gcacggacag ctacgcccag tacatcgccg aactgctggc aaccctgcaa gaacccatgg
      661 tcttagtgga acagcgtctg gatttcagcc gatacgttcc ggacggcttc ggcacgggag
      721 attgcgtgat tgttgcagag aatgttttaa ccgtcatcga tttcaagtat ggcaagggtg
      781 tggcggtatc tgccgaccac aactcgcaga tgatgctgta tgctcttggg gcgttggaac
      841 tattcgatgc cctctatgac attgtagaag ttcggatggt aatctttcag ccaagaatcc
      901 agaacctcag cgaatgcacc ctgtcgctgt cggagttgct gcactgggcg gaaaccgaac
      961 tgaaaccaaa agccgcactt gctgccagag gcgagggcga attctttgcc ggagaacact
     1021 gccgtttctg caagcgaaaa gccacctgcc ggaaacgggc ggagtacaat ctgcaattgg
     1081 caaagtatga ttttgctatg ccggacaagc tgaccgatac cgaaatcgaa acgattttag
     1141 caacggctga ccagctgacc gcttgggtgg cggacgtcaa ggaatacgct ttgcagcagt
     1201 ccttacaggg aaaagcatgg aagaattgga agctggttga aggcagagcc agacgagcat
     1261 attgcagtga aactgcagca gcggaggcgg tacaagctgc tgggttcgac ccatatgaac
     1321 ataaggtaat gggcattatc gcaatgacca gaatgctggg caagaaaaaa ttcgaagaat
     1381 tgttgggaaa tttgcttgtg aaaccacagg gaaagccaac acttgttccg ctatcagaca
     1441 aacgacctgc gtggaatact gcacaggaag atttcaaaga ataaaggagt ttttatcatg
     1501 gcaaagtata tcaatcctgc aaaagtagta accggtgtat gcnnnnnnnn nngattcagc
     1561 tacgccaacc tctgggaagc caaggcgatg gacgagaaca gcaagccgaa gtacagcgtt
     1621 tccctcatca ttcccaagtc ggacacgaaa accatcgaaa agattcgtgc cgccattcag
     1681 gctgcctacg aggaaggaca gggcaagctg aaaggcaaca gcaagtccgt tccgccgctg
     1741 acttccctca agacgccgct tcgggatggt gacttggagc gaccggacga tgaagcctat
     1801 gccaacagtt atttcgtcaa cgccaattcc atcactgctc cgggcatcgt ggatgcagcc
     1861 tgccagcaga ttttagacca cagcgagatt tacagcggtg tctacggcag agccagcatc
     1921 acgttctatg cgttcgctac aaagacctct cggggcattg cctgcggctt gcagaacatc
     1981 cagaagattc gggatggcga gccgctgggt ggccacagcc gtgcagagga cgacttcgca
     2041 accgtagaag acgaggattt tctgaactaa ga
//
LOCUS       DAWBGR010000283         2071 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_12067_length_2071_cov_4.566468, whole genome shotgun sequence.
ACCESSION   DAWBGR010000283 DAWBGR010000000
VERSION     DAWBGR010000283.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12067_length_2071_cov_4.566468"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          520 a          481 c          606 g          464 t
ORIGIN      
        1 gacgaaaaat ttgacgacgc atctgtcgca caatctttgt gcggtattgc cttgagtgtt
       61 cagaacaaac tgtaggctct gtaaaagata gtcgttctag gtttcacctg gaacggctgt
      121 ttttgttttc atgtgacaac accgcacaaa gcccgcctga cggctttgtg cggtgttgcc
      181 atgggtgagc tgtgtcatgg aaaccgaaac atctcctcat ctggtatgca aaaaaaatga
      241 aaaactggca attgcagcaa tgccagaatt gtggtatagt gaagataatc tctccggtac
      301 cagccggagc aaactactat tctggaggaa ttatcatggg aagtcgttat gaactgaata
      361 aaaatctggc acagatgctc aagggcggtg tcattatgga cgttaccaca ccggagcagg
      421 caaagattgc cgaagcagcc ggtgcctgtg ccgttatggc tctggaacgc attccggcag
      481 acattcgtgc agccggcggc gtgtcccgta tgagtgatcc gaaaatgatc cgcggcattc
      541 aggaagctgt cagcattccg gttatggcaa agtgccgtat cggtcatttc gcagaagcac
      601 agatcctgca ggcgatcgag atcgactata tcgacgagag cgaagtgctc tctccggcag
      661 atgacaagta ccacatcgac aagaccaagt tcgatgtgcc gtttgtctgc ggtgcaaagg
      721 atctgggcga ggctttgcgt cgtatcaacg agggtgcttc catgatccgt accaagggtg
      781 aaccgggtac cggtgacgtg gttcaggctg tccgccatat gcgtatgatg caggcagaga
      841 tccgtcgtct tcagtctatg gcagaagatg aactgttcga cgctgccaag gaactgcgtg
      901 tgccgtatga tctggtgcag ttcgtccacg acaacggcaa gctgccggta gtaaactttg
      961 cagcaggcgg cgtggctact ccggcagatg cggctctgat gatgcagctg ggtgcagagg
     1021 gcgtattcgt tggttcgggt atctttaagt ccggcgatcc ggcaaagcgt gccgcagcga
     1081 ttgtcaaggc tgtcaccaac tatcaggacg caaagatgct ggcagaactg tccaccgatc
     1141 tgggcgaagc aatggtcggc attaacgagc aggagatctc cctgctgatg gcggagcgtg
     1201 gtaagtaatg cgaatcggaa ttctggcact gcagggtgca tttgcagagc accgcatgat
     1261 tctggagaaa ctgggagcag aaagctttga gatccggcag aagcgggact tggaacagcc
     1321 gatggacggg ttgatcattc ccggcgggga aagcaccgtc atgggaaagc tgctgaaaga
     1381 actggactgt tatgacgaag ttcgcagccg catcgagaac ggtctgccgg tattcggcac
     1441 ctgtgccggt ctgattctgc tggcaaaaga agcagagggc ggcaaggtgc agctggggac
     1501 aatggacatt gcggctcggc ggaacgccta cggcagacag ctgggcagtt tcgcagtgac
     1561 ggcagaaatg aagggcgtgg gagaagttcc catggtcttt atccgtgccc cgtacatcac
     1621 cgcagtttcc ggcacgacag aggttctggc agaggtagag ggcaagatcg ttgccgccag
     1681 agagggaaac cagctggtca cagcgttcca ccccgaactg acagcagaca cccgcgtgca
     1741 tgactatttc ctgcatatga ttgcagaagc aaaagcgtaa agataacaca caccgcatgg
     1801 tttccgtttt ttcggaaaac acatgcggtg tgtttgttgt gtatcttggg gaatgccctc
     1861 tgttgcattt cccagcaaaa aagcccttga caaatgcggc attctggcgt ataataaaag
     1921 taaagagaga gcataccagt agacggtctg ctcccatgtt aagctatcaa gagataaccg
     1981 tccaagttag cagctcaggg cggttatttc cgtttattgt cgttgttatg gaaaatatcg
     2041 tgcatcaatg ctataatagc aacgatcagg a
//
LOCUS       DAWBGR010000284         2070 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_12075_length_2070_cov_4.054094, whole genome shotgun sequence.
ACCESSION   DAWBGR010000284 DAWBGR010000000
VERSION     DAWBGR010000284.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12075_length_2070_cov_4.054094"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          497 a          509 c          503 g          561 t
ORIGIN      
        1 tcatctgacg aaaaatttga cgacgcatct gtcgcacaat ctttgtgcgg tattacctaa
       61 aataaaactt ttatgcgttt tccagagaca tctgacgcag acgggacaga tccagaccgt
      121 ttactgcttc gtccgtgttt acatctgcac aatcgtaagc ctcttttgtc agagtgactt
      181 cacccagcag gtacttttgc agcagaacaa cgtctacaac agtgacttca ttatccagat
      241 tgacatcacc cggcagatgc tgatctggct tttccggaac gtcggggtca gttaccagtt
      301 cagcagctgc gattgaagag atctgataaa tagtcatatt cacaccggca cgggcaaaca
      361 gatgtgcagc tgtgctcaga tcgctgcccc aagcctttgt gatcgcttcg tgagaatagt
      421 aagcgatacc agcttcttcg tcaatgtcat acgcgttgat ttctgtccag ttgccccagc
      481 tgtcgttgca cagtgccaga atcggagccg caccgccggt gtagcgaatg gcaatttcgc
      541 ggttctcgga aatctgctct gccgtgatat ccagtcccgg tgtgcagttg ccgccattgc
      601 cgtccgaagc gtccagaacc agaccgctgg cattctcata aatgttgtcg ccgctgttga
      661 tgtctgcatg tttccatgta ggcaggtggc tttcgctgcc ccagacaatg ctgtcgtcct
      721 gcatcacagt catcatagca tcaacaaccg gttcagaagc ctcataccaa gtgttgttgc
      781 tgcggtctac atagccgtga gcctctgaag cagatgtgga attggtgata gcattgttgt
      841 cccaaagcac gcaggggatt cccagctttt ttgtccatgt cagataatag gttgcccagt
      901 cgcatcttgc ggcagtattg ttaaagttgg aagcactgaa ttcgcccagt accaccggaa
      961 tatccttgtc tgtgaaatag taccggatgt cagagaacag cgtatccaga tcactctgat
     1021 agttgccgga gaaagtggtg tggtcaccat cgcccatggc aaatgcataa ggggtgtacg
     1081 catggagcga aactgccaca tagtcatcgt ccggcacgtc cagggcagag taggaacttg
     1141 cgtatgccga agcagcataa gacgggatca tcagcagacg ggtgttctga taaggagaat
     1201 caatggaacg taccgtttcc acgaaatcgt tgtccagctt gttgactact tcatagcatt
     1261 ccgcgttgcc ctgccattcg taggaagctc cggtctgtcg cggttcgttc atgccctcga
     1321 agatcagatg ctggtcgtag tccttgaagt aggtggcgat ctgtacccac atctgcttca
     1381 gccgttcgga catttcctca taagcagtgg gaaagtcaga acgattgatc cactcctcgt
     1441 ggtgcagatt cagaatgaca tacatatcct gattataggc gtagtccgct acttccttga
     1501 cacgctgcaa ccactgctcg ctgatcgtgt aaacaggctt gccgttttcg tcagtagtcg
     1561 ttgtgacatt ctgataccat gtggtgggaa cacgaatggt gttgaatccc tttgccttga
     1621 cggtgtcgat cagttcctgc gtaactctgg gattgcccca gctggtttca ccgctgtatc
     1681 cggtggaatc cagagagttg ccaatgttcc agccgatcgt catgttttcc gtaatatcat
     1741 cggcggtcat ggttccctca gcggaagcgg ttgtctggaa aaatgtcatg ccggaaagca
     1801 gcatgactgt ggcagtaccg gcagcaaata acttgcgaaa ctgaaatagc atagttttcc
     1861 tccttggaac gtgttctttt cacacatcat ttttcatcaa acgaaatgcc ggaaaaacgg
     1921 catattttct tttattatat catgaaggaa tggaaaagtc aatagatttt gattcacttt
     1981 cacaattttt tctgtacaaa tttgcagtag cattcttagg gcaataccgc acaaagattg
     2041 tgcgacagat gcgtcgtcaa atttttcgtc
//
LOCUS       DAWBGR010000285         2045 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_12262_length_2045_cov_4.648744, whole genome shotgun sequence.
ACCESSION   DAWBGR010000285 DAWBGR010000000
VERSION     DAWBGR010000285.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12262_length_2045_cov_4.648744"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          478 a          579 c          563 g          425 t
ORIGIN      
        1 aagggaaatt tgcaagcaga tggcgtgcaa agtcgccaga cgctctttgt gcggtgttgc
       61 cctaaggcaa tactgcacaa gacctgtgcg gcattgcctt gacagataaa ggagggattc
      121 cccatgctgg tcaagcgtgg cgaaatttat tatgcagacc tgagccccgt tgtcggctct
      181 gaacagggcg gtgtccgacc ggtacttatt gtgcaaaacg atgtcggaaa ccggcacagc
      241 cccaccgtga ttgcggcggc gatcaccagc cggcgggaaa aagccaagct gcccacccac
      301 atcgacgtgc aggcagccac ctgcggactg accaaggaca gcgtggtgct gttggagcag
      361 atccgcacac tggacaaacg gcggctgaaa gaccgcatgg gcgagctgga cgaaccgtcc
      421 atgacacggg tagaccacgc cctgcaagtc agtttcggac tgccgcttta aagtacaaaa
      481 agagcgtaca cccacatcgg gcatacgctc tttctctttg caaaatggaa ttcgctttcg
      541 cttactttgc agcgttcagc ttctttgcca gctgggactt cttacgagca gctgcattct
      601 tgtgcatcag accctttgca caagcctgat cgacacgctt gatggcaacg cgtacagcag
      661 tttccttgtc agcagcattt gctgcacaag ctgcgtcagc cttcttgatc atggtcttca
      721 ggttagactt gcgagcctta ttggcagcag tcttggttgc acttactttt acacgcttct
      781 tagcagaacg aatattcggc atagttcaga tctccttttt tacaatcaaa aataatgggt
      841 agaattccct cgaatcaccg gattctgtac gtcatacact aatgacagca ttgacacggt
      901 actgtcattt cgtggggaaa cttccccgca attggaacga ccaaatgctg gcattcttat
      961 cagaacgccg tgactaaatt ctattataac acagcccgtc cgaaaatgca ataggtattt
     1021 tcaaggaacg ccacaatttt ttcgcgtgaa aagccagcaa tttgcacaaa aacacaggag
     1081 gttatgccac atggagccaa ttcgcactga cctagccgtc gaacgcaccg ccgccgcccc
     1141 cagtctgccg ggggtcacac aggaaacccg tgggaacgcc ttttccgtca cagagatccg
     1201 catcaagaat gatgtctgcg gtgcccctat cggaaagcct gccggacgat atgtcacact
     1261 ggaagcccct gccatttccc gcagcagtgc caactatcag gaaatggcag aggagctcgc
     1321 cgcggaactg ggaaaatttc tgccgcagga gggctgtgtt tttctggcag ggctgggaaa
     1381 ccgtgccatt acgccagacg ctctgggtcc ccgcgtcgct tcgaaagtgc tggcaacgcg
     1441 gcacctccgc accgccctca ctgcggagga actcacctat ctaccggaac tgcggccagt
     1501 ctgtgtgctt gccggcggtg tgctggggca gaccggcatg gagtcggcag aactgatcga
     1561 agcggtctgc aaacagatcc ggccggcggc agtggtggcg gtagatgctc tggcgtgtgc
     1621 ggaactctcc cgactgggca cgacaattca gatgagtgac agcggcattt cccccggcag
     1681 cggcgtggca aatcaccgtg cggaactctc ccgccggacg ctgggggtgc cggtgatcgc
     1741 actggggatt cccactgtgg tagacctcca caccgccgta cagggcatct acggcaaaag
     1801 cgttcccaag gatcgcccca acatgatggt cacgccacgg gacatcgacc ggctgatcga
     1861 ccacgccggc gatctgctgg cgtgtgccct caacatggca ctccatgacg gcatgacctt
     1921 tgcacaggcg gagggactgg ggtgaaccct atggaaacgc tgaataaatt gcagtgataa
     1981 acgcttgaca aacgaatttc atgatagcct cccctgaaag gggaggggga ccgccgttag
     2041 gcggt
//
LOCUS       DAWBGR010000286         2018 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_12482_length_2018_cov_5.498217, whole genome shotgun sequence.
ACCESSION   DAWBGR010000286 DAWBGR010000000
VERSION     DAWBGR010000286.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12482_length_2018_cov_5.498217"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          593 a          430 c          545 g          450 t
ORIGIN      
        1 gtcatattgc ccagaaatcc cttgccatac atacagtatg cctgcggaat ttctatgcaa
       61 tctgccgaaa aatctgacta cgcatcttac gcacaagctt tattcagtgt ttcctaagca
      121 ttcgtaaaac agaaaagaaa catactttta cccgaaagga gaaacgatca tggaaaaatt
      181 cgtaccattt gaaaaactaa gcaagaagaa acagaaagca ctgaacgacc agaaacgcgg
      241 cgattgggga aatgtcaacc ctgccacaca ggtcttgccg gacaagaaga aatattccag
      301 aaaacagaaa cacaagggga gtgaactcga tgattaaaat agaaggcact tataacacag
      361 cgatcgtcta tgcagatacc atagaagcgg gtgccgaggg gcagattcag aagctggtat
      421 cacaggaatt caccaagggc agccggattc gcattatgcc tgatgtacac gcaggcaagg
      481 gctgtaccat tggaacgacc atgaccatta cggataagat cgtgccgaat ctggtgggtg
      541 tagacattgg ctgcggcatg gaaacggtgg aattgaaagc caagagaatc aacctgccgg
      601 aactggacag tttcattcac aagaatattc cctgcggctt tgagattcgc agtgccgcac
      661 acaaatatat cgccaatacc agactggaag aactgtactg taaagaagca gtttccatgc
      721 cccgtgcgga atgcagtctg gggacactgg gcggcggcaa ccactttatt gaagtggaca
      781 aggcagagga cggcacactg tatctggtag tgcattccgg cagccgaaat gccggcttac
      841 aggtggcaaa gtactatcag gacaaggcgt atgctctgct gaaagaaaac ggggaaattc
      901 cctatgaatt ggcatactgt gaagggaaac tgctggagca gtatcttcac gatatgcaga
      961 tcatgcagga attcgccgac tggaaccgtc gggcaattac ggatactatt ctgaaaggct
     1021 gtaagctgaa agaacaagga cggttcacga cgattcataa ctatatcgat atagaaaacc
     1081 ggattcttcg gaaaggggcg gtttctgcac aggcagagga gttgctgctg attccgatca
     1141 atatgcggga cggttcgctg atttgcagag gaaagggcaa tgccgagtgg aattgttctg
     1201 ctcctcacgg agccggcaga ctgatgagcc ggagcgatgc caagaattct tttaccgtgc
     1261 atgcctatcg gaaggaaatg gagggcattt attcctcgac tgtcagtgca gaaacgctgg
     1321 acgaatgccc tatggcatac aagagcaagg aagccattct ggaacaaatc acgccgaccg
     1381 cagaagtggt gcgggaaatc cagccggtct ataactttaa ggcggagtga ttgaatatgt
     1441 atggcatggt tttaggtggt gcgtggctgc tttcggtatc ggaaacggca attgaagtat
     1501 tgcatcaccc ctctgaaact tttgagtttg attctgttgt gaagctggtg cagcaatacg
     1561 gaaacgccag tcaacagaag ctggcggaac agtatgcctg cacgccgaac gacacgctga
     1621 aacggcagct gattgcggtc taccaggaca cgtggtgcaa agtgcgtgcg tggggtgtgt
     1681 ttcaggagac agtgactttt cgaattgcct ctgtaacata ccagtggtat ccggcgatca
     1741 cagcatttct gcgtgccaac ccgcgatttc gggaatcgaa aattacggtg gaaccggagc
     1801 aggcatggga gaaaagagca tatattgatg cagtgccata tgatgtgatt ttgaatccgg
     1861 cgaatcggga gcgttttcca aaagcattga catctggaac tgacttcaga tagaatgttt
     1921 gcgtgaagtg atgattttat gccattgttc gcagtatgga atcgtaaagg caataccgca
     1981 caaagattgt gcgacagatg cgtcgtcaaa tttttcgt
//
LOCUS       DAWBGR010000287         2008 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_12566_length_2008_cov_3.151050, whole genome shotgun sequence.
ACCESSION   DAWBGR010000287 DAWBGR010000000
VERSION     DAWBGR010000287.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12566_length_2008_cov_3.151050"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          619 a          387 c          487 g          515 t
ORIGIN      
        1 atctgccgaa aaatctgact acgcatctta cgcacaagct ttattcagtg tttcctttaa
       61 ggaatcatcg tgaaaggaac cattccccat gttacagcaa acctatattg accgactgat
      121 taccaccgca ctggacgaag atattcacta tatcgatgtg accactgaca atctgctgga
      181 cgacgcacac accagtgagg cgtattatct ggcaaaggac agcggtgtgc tctgcggcat
      241 tgacattgcc aagcgggtgt ttgaactggt tggcggcgac ttcaccatgg atattcgcat
      301 tcatgacggc gaaccggtga aaaagggcga tattatcgcc accatgaccg gcagcaccaa
      361 gaccctgctg aaaggcgaac ggactgccct gaacattctc cagcacatga gcggcattgc
      421 cactgccaca caccagtgcg tggagctggt ggctggcacc aacgcccaga tcaccgatac
      481 ccgcaagacc ctgccggggc tgcgtccgct gcaaaagtat gccgtgacgg tgggcggcgg
      541 caagaaccac cggtacaatc tgtctgacgg tgccatgctg aaggacaacc acatcgatgc
      601 ttacggcggc attacgccgg ctgtggctgc cctgcggaaa aagatcgggc acatggtgaa
      661 gatcgaagtg gaagtgcgga cactggaaga actggaagaa gccctgtctg ccggtgcgga
      721 catcatcatg ctggacaaca tgagctgcga ggatatgacc aaggcagtgc agcgtgtgga
      781 cggcaaggtg ctgctggaag cttccggcaa cgtgaccgcg gaaaccattc ggggcattgc
      841 agaaaccggc gtggacatca tctcgctggg agcactgact cattctgtca agtgctttga
      901 tatttccatg cggatctctg caaagaaata agaattgaat ttttcataag agcattcccg
      961 gtgcagcatt gctgtgccgg gaatgcagaa acaagtactg tttgcagaat gacggaaaag
     1021 catggcacaa caaaagttct aaaataactt tacttttctt ttgaaatgtg atataattaa
     1081 atggaaattg gtctaccata tgtatttccg atattgtcat atgcttaaaa aagaggaaaa
     1141 ttatgaaaaa agaaattgca gaagaacgta tgcaaaatat gaaaaatgcg tatttgaagt
     1201 tggacagtgt attgaatcgt ttgcctgatg ctattccaga aaagatgaag agcgttttgc
     1261 gtgacaaaat attaggagac aaacagttaa aagaattgat ggaaggcatt gatgagcata
     1321 gaccacctcg ctttttcttg atcggtcgga ctggtgttgg aaaaagtagc ttgattaacg
     1381 ctttatgtgg gagttacttg gcaaaggtta gtgatataga aagttgtact tctaatgtcg
     1441 atatttacca ttgtatggat ggggatagag tccttttaga tatattagat actcgtggaa
     1501 ttgcagagtg caataaaatc aatgattctt atgatgctga aaagcaatta atcgatcaag
     1561 tggttgactt ttgtccagat gtagctattt ttgttttaaa tgctacaact cgtgatgaca
     1621 gtattttaca agacatagaa cttttgaaaa aggtgctgaa aaagtatcag gcgataaata
     1681 agatagaact tccagttgtt gctgttgtta acaaagtgga tgaaataccg ccggcacgta
     1741 ttaaaaatcc aaaagagtat acagaaaata agataaatag tatttctgaa aatgtaaaaa
     1801 gatataaaac tacaatcgtt tctaatggtc ttaaaattca cagcattgtt ccggtgtctt
     1861 cgttgatgga ttggcaaacg ccagatggaa ttgaagttaa tgcagataac attaataatt
     1921 ttccgaagag tgacattgaa aatctgcaaa ttcaatttga cggcagattt caaattgaag
     1981 ttttattaaa tgatttggag gatgccat
//
LOCUS       DAWBGR010000288         2003 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_12603_length_2003_cov_5.548768, whole genome shotgun sequence.
ACCESSION   DAWBGR010000288 DAWBGR010000000
VERSION     DAWBGR010000288.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12603_length_2003_cov_5.548768"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          429 a          547 c          534 g          493 t
ORIGIN      
        1 ccgataatct cttacaattc cgactttcat aacgttactt aaattataca tgattcacaa
       61 gtttttgtca agtgttattt tttcggaaac attggcatgt tacgcttcca ccggtgcaaa
      121 ccgaatccgg atctgcggca gttcgtggag aacggtgtcg cagtggtaaa gcccgtcttt
      181 cacagacagg tcattctcac cggttgccgg cggtgcgaaa attttcaagg tcaggtcagc
      241 ttcgccctgc aaaggcttct cgaagaatgc cgccatcaga tctaccgtct tggtcttggg
      301 tgccttatag aaccacttgc cgttcagttc cagcatcagg ttgctttcct cggtaaaggt
      361 cagttcgcag aatgtggggt tcttctcaaa atgcagcggg atttccagac cttccgcgtc
      421 ctcggagttg ctccagcgga gtgtcaccgg cagctgtacc tcgcctgccc gacgggtgat
      481 ctggggcctg aaccagtcac gctgaatgat ttccttatag gtatccagca ccggctgtgc
      541 gattcccaca tttgcattgt tgggatcctc gatctccagc cccagtctgc cgcggtcgcg
      601 gaactggtag aacgtgaacg cactgaacca gtccgcctgt tcccgttcca gtgtatcgca
      661 gaattccttt accatgtctg cctgttcata aggaccggtc acgtcgccgt cagcgttgaa
      721 ctctgccata gtcataggct tttccacgcc gccgttgaga taacggaacc gttcgaagct
      781 gcgtttggtt ttctcgtagg tgtccgccac agtgccgcgg ctgtgagtag tgccgccgtg
      841 ttctgccacg tcatagggcc agccccagtg cagtgccatg tacttgtcca ccgaccagat
      901 gtcagctgcg ggaatggtct gtgcgaactc ggcttccttt tccatttcct ccttggagag
      961 atcctcgatg ccgccgatgc agataatggt ctgcacgttg ggagcatgct ccttgatgat
     1021 acggctggca cggacaaaaa agtctgccac ttcctgatag gtgcagcgtt tgttgaacga
     1081 gaaccagttg cccgttgcct cgtggttgat acgcaggaac actctgccga aggacttcag
     1141 atcctcgccg atcgccgcca gatgttcatc ggaaacgtgg ggatcaatgg tcagtgtcag
     1201 ggaaacgtcc tgtccgtgct gccggatctc ccgcatgtag ttgaacggtt cgccgtcggt
     1261 gctgccgccc agtccgccct tgtaggtgaa atagtcatcg tagggataat cccacgcaaa
     1321 gctgacatca gggctggcgt gtaccacgct ggcacgcccc ggccagcctt cgtccagcgg
     1381 ataatagcag tacatcaggc tgatactgcg gtgaggtctg cccattttcg ccagaatgta
     1441 atcctgatcg acatattcac cccgttcgct gccgtccccc agatcaacgg cacactttgc
     1501 cttgcccatg tgaatgcggt aaacttccat gtatattctc cttttcggca gagcatgctt
     1561 caacagcacg ctctgatgtt tctggcaata ctgcacattc tgtgcgattg ttcttggttt
     1621 attataccga attccggcgg tgctgtcaag ggattttttg agaacaggaa taagcttctc
     1681 ctaaaataga agcctataca ctaaaaggaa cggaaataaa aaacctcccc tgaaaggggt
     1741 gactccccac ggggtgggga gatgcccgca gggcagaggg gaccgctcag tacgagggga
     1801 accgtcgtca gacggtggag gggttcttct gtggaatctt ttgcataatg tgtcaggtta
     1861 cacggaagta cccctcagtc acctgcggtg acagctcccc taaagggaag ccatatacac
     1921 aaaagggagc ctgaataaaa atagcctccc ctgaaggggt gactccccac ggggtgggga
     1981 gatgcccgca gggcagaggg gac
//
LOCUS       DAWBGR010000289         1999 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_12632_length_1999_cov_4.443416, whole genome shotgun sequence.
ACCESSION   DAWBGR010000289 DAWBGR010000000
VERSION     DAWBGR010000289.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12632_length_1999_cov_4.443416"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          483 a          469 c          527 g          520 t
ORIGIN      
        1 ccttcagagc ctgtttcgta tcttcccgca cgcatcttgc gtgcatattt tcccttcatc
       61 tctcaaaaaa tttgacaacg catctgtcgc acaatctttg tgcggtattg ccttaacata
      121 ataacatagc atatttaaaa ggactgaccg atttccccgg tcagtttttt tatagcactg
      181 ccgccgcacc ccaccaccac tttttgtcaa ttttataaat tatgtcactt ctcccgcgaa
      241 acacttgaaa actattggca atcatgctat actattacca taagggaaac aatcgaggtt
      301 tgacaaaagg aggtgtgccc gatgaaatgg ctgtcaaaga aaagtctgct gatctgtctg
      361 acactgctga tgctggttgt ggtagtggtt tccatgatcg ttaccacggg catgcctgtg
      421 agagaagatg cggtgaatat ggtgctggat gactggacta cggagcaggt cattgacccg
      481 ccggacgagc cttattacag cggagaaaag acagtctatc agctggtgat gtcccataaa
      541 attacatcgg aaatgcgtgg gaaagtgatc tctttccgga caaatgattc ctatgtggat
      601 gcctacctca ctgacgtgac aggcacgccg gaagacgatg cagtacacat ctaccacttc
      661 ggggaacgct taaagatctg cgattcaccg gggacttata cccattttat ccgactgccg
      721 gagtccgcag tcgggcaggt gaccatttgt gtggaaacgg tatatgccaa caagttcatg
      781 ccctcttatg aggtggcgat cggcacccag aacgaactgc tgttcaacta cctgcggcag
      841 aatctgctga gcggcatttt caacgtagca atgctgcttg ccggtgtgct gctgctggtg
      901 atctatacgg tgggcagagc gaagcatgtt tctgcaccgg aaactctgtc gctgggctgc
      961 ctttcgatcg tatttgcggt gaatctgaac tgcccgctgt ttctgaatca gttcctgtat
     1021 caaaatgcag tggtgcagta ttatgtcaac tacttttcgc tgtttctgct gccgctgctg
     1081 gcgatgtttt attttgagga tattgtgccg gatctccgca tcaaatggcc gttttatagc
     1141 tttctggtgc tggagtgcgt gttgagtgtt ctgcacttca ccggcatcgc gtcctatacc
     1201 cgcaccgtta aagtttttag tgcagcactg ggcatacttg ctgtgatgtt tattgtgatg
     1261 atcgcaaagc gataccggca aatggagaag atcaaccgca tcagtttact gctcctgctt
     1321 gcctttgtgt gcagcaatgt tctgttcttc attttcgttt ccaccgtagg cgaccagacg
     1381 ttcattatcc ggacaggctt cctgctgtat ctggcacttt ccgtggtgaa cggactgcgg
     1441 aaactgatgg tggagatgaa tcgggaacgg gaaaccaggc tgctccagac actggcgtat
     1501 acggacaacc tgaccaagct ggggaaccgc tatgctctgg aacgcgatgc aagaaagtgc
     1561 gtgctggaac gtaccagtat tgtgtccatg gatctgaacc tgctgaaaat ggtgaatgac
     1621 acctttggac atgccggcgg ggatatgctg ctgcaaagtg ccgccaagtg tatgacttcc
     1681 gtgtataatc aggtgtatcg ggtaggcggt gatgagttca ttgccctgct ggacggcaaa
     1741 gacagtgacg acttgcagcg gctttacgac cggctgaagc agaagatcac tgagatgaac
     1801 gacagccgga aagactacgg ggcattctca cgggaggcgg agttccggtt aagcattgca
     1861 gtagggtatt ccgcttatgc ggcaggggac agcagctatg agcagatcat gagccgtgca
     1921 gatacggcga tgtacgcaga aaaaaagaca atgcatcacg gcagaagccg gtgattttaa
     1981 aggcaacacc gcataaaga
//
LOCUS       DAWBGR010000290         1989 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_12726_length_1989_cov_3.761634, whole genome shotgun sequence.
ACCESSION   DAWBGR010000290 DAWBGR010000000
VERSION     DAWBGR010000290.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12726_length_1989_cov_3.761634"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          424 a          545 c          513 g          507 t
ORIGIN      
        1 ctcagtcaag cctgcggctt gccagctccc ctaaagggga gcctatgcac aaaagggaaa
       61 gcttgaataa aaatcagcct cccctttagg ggaggggaac cgccgttagg cggtggaggg
      121 gttctttcgc acgaagtttt tgacataacc tctcttttta aaaagtgtta gtggctttgg
      181 tatttgcagt tttcacaaat gtttttccag ttttcttttt ccttaaactt ttcggggata
      241 ctttcttctt tcaaatcttg attttccatg caatccaccg gatttatagg ggcattaatc
      301 aatgggcaaa tcactttttt ccattttttg tgtacctccg atacgtcaca tacacatacg
      361 ggatctcacc gtcatggcgt tcttctgcga ctcgtgcaaa ctgtcctgcc agcagcggcg
      421 gaaagaacac gtccccttga aagaacccgt cgatctccgt gagatacagc atttccgcca
      481 gcggcattgc ctccgcgtag agccgtgcac cgccgctgat aaagacccat tccgtctttg
      541 ccgccgccag agcctctgcc aacgtgggaa cggtttgcag atgggaaccg gaaaactgcc
      601 ggctggcgga aaccacaatg gtttcccgac agggcagcgg gtggccgatc tcctcatagg
      661 tacgccgccc gaagatcacc gtctgtccgg tggtaagctg ccggaaccgc tgctgctctc
      721 cggcaatgtt ccacgggatt ttgccgtttt ttccaatggc acggctccgg tcgcaggcgg
      781 cgatcagtgc aatgtgtttc atgacagcag catcaccagc atcatgatca gagaaaagat
      841 gatagaggct cccatctgaa tgccgagcat tgccaccagg aaccagccgt tcacgccgcc
      901 ctcctgccgc agtctgcggc gtttcatatc cggtgaaaca tcttcctgta ccagcttgtg
      961 cacctttcgc agcacaaagc ggtaataaag gcggttcccg aaaagcccca gcagaatgct
     1021 gatgatgatc tccagataac tgctgatcat ggcagcgttg tacagcaggg tttcgtgagc
     1081 gtccagcctt ctgctgaatg cctgaaatgt ttgcagcagc tccgccggtg ctccggtctg
     1141 ccgcagtgtc ggcagatagt ccggcagcag tatctgcatt tgctgtaagg actggggaat
     1201 tttcagcagg agcatgatgc ccatgacgat cagagccgcc gcccacattt tccggtacgc
     1261 cagataatac tggggaaaga gcaggcaggg gaagttcaga gaaattctgc cgcccttgct
     1321 gaaccgcagg aacttcggca gatagtacag gttgttctgc tccacgaagt tcgccacatc
     1381 ttccagccgt tcgccgtcca gcttctgttc ctcgttcagc ccgcaggtag gatcagcact
     1441 gcccatgcgt tctgcggctt gctgcaactg ttcgcggagc gtcggcgtga cctcctgctg
     1501 ttcctccggc cggagaaacg gcttgccgca gtgactgcag tgggtggcat cttccggatt
     1561 gccggtgccg cagtcggggc agatcttggt gttcttgact tcttccctgg gcttggtgga
     1621 ttcccagctt tttccggact catgaagcgg caggttgatg caggcaccgt ttttctgcca
     1681 gcaggcacgg tgatacggcg tgccgcattc cggacaaact acaacgtcat ctgcatctgt
     1741 gaagacctgt ccgcagacga tgcatttttc tccggtgtgt attcccatag cgtatccctc
     1801 ttttctaagt gtttcttcaa caactttttc cggtgtgttg cctgaagttt cagccgttac
     1861 aacccaccag caactttatt cacggaaatc aattttcaaa aatatgttga tttaaattgt
     1921 gccaaattat gaattttgac aagtgaggct cccctgaaag gggagctggc agtgcgtagc
     1981 actgactga
//
LOCUS       DAWBGR010000291         1988 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_12741_length_1988_cov_4.554578, whole genome shotgun sequence.
ACCESSION   DAWBGR010000291 DAWBGR010000000
VERSION     DAWBGR010000291.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12741_length_1988_cov_4.554578"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          433 a          494 c          540 g          521 t
ORIGIN      
        1 gtatatttct attataccat attgtctaac aaaaggggag catttcaata ttcctgtacc
       61 ggtttctttg ttttattgat tcagtgtgaa aatcacgcgt ccacgacttc gtccagtgtg
      121 ggctgtccca gttccagata gtaatggttc agatactggt ggtaggattc ctgaaaggtt
      181 tcatcgtttt ctttcagctt gaacagatac ttgtggatat tctgctgttc gtcgatttcg
      241 atcagcacgc cggcaatgtt ggaacaatgg tcggaaatgc gttcgatgtt ggtgagcaga
      301 tcctgataga tgtagcccag ttccacggtg cattcttcgt tttgcagccg cttgatgtgg
      361 cggtttttca gttccagatt cagggtgtcg ataacttctt ccagcggctc tacctggtgt
      421 gcaatggcga ggtcgtcctt ttcgaacgcc tgatatgcca tgttcagtgt ttccagcaga
      481 gcatcacaca gggtacgcag ttcctttgcg gcatcgccgg agaagtgcag ccccttttcg
      541 tggagttcgt gtgccgattc catgatattc accgcatgat caccaattcg ctcgaaattg
      601 ccgatgcagt gcagcatttt cgaaactgtc cggctgtcct tggaggaaag gctgtgcttg
      661 ctgatgcgta ccagataggc gttcagctta tcctcgtatt tgtcgatgcg gttttcgttt
      721 tcctcaattt gttcttcggt ggctttgtca tactggaaca gcatgttgat cgccagtttc
      781 attgtggtct tggacaactc tgccatttcc atggtcacac cgtgacagac ttcaatggca
      841 actgctggtg ttgccagcag gcgttcgtcc agcatagcat gcttttcctt ggcttccttt
      901 tcctttttgc tgtcccgtac cagcgtatac gccagctttt ccagcagctt ggtgaacggc
      961 agcagcattg ctgtagtgac aatattgaag atcgtgtgta ccagagccac gccagctgga
     1021 tcaattgcag agccgacaaa ggcgaagtcc agcagccagt acagtccgta gaatatcggc
     1081 agaatcacca gtgtgccgat gcagttaaag cagatgtgca cacatgccac ccgcttggca
     1141 ctgcggttta cgccgatgct ggagagcagg gcggtgacac aggtaccgat gttctgtccc
     1201 atgatgatcg ggatcgccat gccgtaggtg acctgtccgg tgagggagag tgcctgtagg
     1261 attcccacag aagcggcgga actctggatc acgccggtga aaacggctcc taccagaacg
     1321 cccatgatcg ggttccggaa cgcggtgaga atgtgggaga actgcgggga atctgccagc
     1381 gggctgacgg ctccgctcat cagggtcatg cccgtcatca gcacggagaa tcccaccatg
     1441 atcttgccga tgtccttggt gcggttatgc ttggcagtca tgatcataat gacaccgatc
     1501 agagcaatga tcggggagaa cgattccggc ttcagcattt tcaggaaggg attgtccccc
     1561 tcgatgccgg aaaggctcag gatccatgca gtcagcgtgg tgcccacatt ggagcccatg
     1621 agtacgccga tcgtctggct cagttccatg atgccggagt tgacgagccc gaccagcatg
     1681 acggtcaggg cggaggagga ctgaatggca atggtaatgc ccatgcccag caacaggctc
     1741 ttgaacttgt tggaagtcat ctttttcaga acgatttcca gcttgccgcc ggcgagtttc
     1801 tccagaccgg aggacatgac gttcatgccg tacaggaaga acgccagacc gcccagcagg
     1861 gtaatgacgg aaaaaatatc cataggtttc gactccttat tgctgccccg tgaaaacagg
     1921 ggcatttttc tggggcaata ccgcacaaag attgtgcgac agatgcgtcg tcaaattttt
     1981 cgtcagat
//
LOCUS       DAWBGR010000292         1979 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_12824_length_1979_cov_3.970374, whole genome shotgun sequence.
ACCESSION   DAWBGR010000292 DAWBGR010000000
VERSION     DAWBGR010000292.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12824_length_1979_cov_3.970374"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          636 a          402 c          520 g          421 t
ORIGIN      
        1 tgacgaaaaa tttgacgacg catctgtcgc acaatctttg tgcggtattg cctatgtgtc
       61 ccttttgtac atacctacta taccacaaat gtccgtcgaa atcaattgaa aatcaaagtt
      121 ttctgagaat ctgaaaaaaa tttcgtgaaa gtatggactt atgcgaaaaa atatagtata
      181 ataaaaaagt atcgggcaag acagttcggg gaatgctccg gctttcggcg gcgtttcctt
      241 tgtcctgctg ctggcttgga atgcggtttg aaccggcatt tcgggaccgt ttattttatt
      301 cggaggtatc aaaactatgt tcaaatggaa aaaggctgcc gctgctttgg cggctgccgc
      361 aatgactgtt gctggtgcag gctgcactgt cggcagcggt tctgcgtatg caatgacaat
      421 cgacggcgag cagatcaatg caggtatcta tatctactat tcctatgcat cgtacatgga
      481 actgacacag accttgcagt cccagaattc ggaactggac gtaaaggacg acaatgtcgt
      541 aaaagaacag aaaatggacg gtgtcagctc ggaagaatgg atcaagaaca aggcactgga
      601 atattgccag cggtatgtgg caattgaaaa gaagttcgac gaactggatc tgtccctgac
      661 agaagaagag tccaaagacg tggagaacac cattgattct ttctgggata ccaatggcga
      721 gacctacgag aaaaacggca tttccaagaa ttctgtcaag agcgttctgg aaaatacgta
      781 tatgaccaac gaagtattcc tctattatta cggcgttgac ggcgaagaag gcactaccga
      841 ggacgatctg aagcagtact atgaggaaaa caatgcccgt gtccgctaca tcaagtttga
      901 tctgacagac ggaaacggcg aggctctgga tgatgccggc aagaaggaaa tgaaggcaaa
      961 ggtcgaggac tatctgggcg agatcaacgc actcaagggc gatgaggacg caatggaaga
     1021 cgaaatggac aacgtgcaga gcgactataa cgcttatgtg acttccattt ccgaggaagc
     1081 tgccgcagca acagccactt ctgcaaccga tgcggacggc aacgagatcg ctgcaacaac
     1141 agaagcaact actgtcgctg ctgaaactac cacagaaact accgcagccg cagaggaaag
     1201 tgctgctaca gaaactgccg gtgcagaaga agctgcggaa accactgcgg cagaagaatc
     1261 tgcttcggaa gaaactacca cgacagaagc agctgtggaa accgatgaaa acggctctga
     1321 agtgactacc accacgactg ctccctatgc aaatgaacag atcattgcaa aggtgactac
     1381 aaaagagaac accaaggaag aagatgtgac ctataatccg tgcaagaatg tatacgacta
     1441 tgtattcggc gacggacaga agaactatgg cgtggcaact gtggtagaag atgacacggc
     1501 ttactatatc gtaatgtgcc gtgacatcaa ggagcgtatg acagaggacg acctgtggac
     1561 agaaacacag cagaatactg tgatctctca ggcatatagt gatgcgtttg aggatatgct
     1621 ggacggctgg actggcgagc agaaggttga gaagaacgac agtgcgatca agaagtacga
     1681 tgcgttcaag atcgatatgg acagcagcag tcagagtgca taacagaaaa tttgaataga
     1741 attatgcgaa agcccgattg gaaacagtcg ggctttttgt atgcatttga ggagaagata
     1801 tgcatactgc acaattcgga gaaatatatg ttcacaattc tctacttgac acagtagaag
     1861 aagtgtgata taataataaa actgtcgcac gagagcgaaa gtcaagagaa taaaggacaa
     1921 cggaaactgt gaaacggatc gggagatcga cagttagcgg ttggagttgt tcaagaaaa
//
LOCUS       DAWBGR010000293         1931 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_13244_length_1931_cov_3.923774, whole genome shotgun sequence.
ACCESSION   DAWBGR010000293 DAWBGR010000000
VERSION     DAWBGR010000293.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13244_length_1931_cov_3.923774"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          460 a          423 c          506 g          542 t
ORIGIN      
        1 cgaaccagtg gcttactgct tagaaggcag ttactctatc cagctgagtt aaaggcgctt
       61 ttggagcggg tgatgggaat cgaacccacg taaccagctt ggaaggctgg aattctacca
      121 ttgaactaca cccgcatggc gtttatcggt ttcgtttatc tcgtcaaccg acttatatat
      181 tatagcacag tacaacgaat ttgtcaagct tatttttcaa tgtttttcca tcaatttatg
      241 cgattaaaag tagaattctg caaaataaac gaaagtaaat ggagcgagtc acactaaaca
      301 tgccgaaaac tgatttttac gcttcttcat ttcgatttgc tattcagacg aaaatgtgct
      361 ataataaaaa tgacagcatt caggaaacac tgcataaagc ttgtacgtaa gatgcacggt
      421 cagatttttc ggcagattgc gtgcaaagcc gtcaggcgga ttttatgcag tgtttccgta
      481 atatatagaa agaggtattc gctatgggaa tagaaaaagt tcctgccaag atcgatatgc
      541 tgcacgggtc actttgggac aagctgccac gctttgcact tccggttgca gccacggcga
      601 ttctggaaca gctgtttaat gcatcggata ttgccattgt aggcaatttt tcaagcgggg
      661 ataaaactgt tgccgttgcg gcggtagggg caaacagtcc gctgatcggg ctgattctaa
      721 acttatttat tggtattgca ctgggtgcca acgtcgtaat tgccaatgct atcggcagaa
      781 aggacatgcg gtccgtcaag aaagccgtac ataccgcctt aatcgtagcg gtgctcagcg
      841 gcattgtggt aacagttatc ggggaactgg tagcaggaaa agtcatggga ctgcttcacg
      901 tgccggacga tgtttttccg gaggcattgc tgtatctgcg gatttatctg ttggggctgc
      961 cggtcatttt tctctacaat tttgaagcgg cagtgttccg gagcatcggc gatacgaaga
     1021 ttccattgca ggctctattg atttccggcg ttttgaacgt gattctgaac ctgttctttg
     1081 tcatcgtgct gcatatgacc gtcaacggcg tggcaattgc cacagtaatt gccaacggtg
     1141 tcagctcttt gattttgctt tggaaactgc tgcatactga caaatgcgtt cgcgtgtcac
     1201 ggagcgactt gtgcattgac cggcagagtt tgcttcgcat tgtgcaaatc ggacttccgg
     1261 cgggaattca gagtgctgtt tttgcaatgg caaacattgt gatacagtct gctatcaaca
     1321 gtctgggaac tacggtgatc gcggcttcca gtgcggcgtt caatctggag atactggcgt
     1381 actacgtgct gaattcgttc agtcaggcgt gcacgacgtt tgtgggacaa aattacgggg
     1441 caggcaatat gcggcgttgc cgaaaggtac tggggctgtg tctggcggaa gatgccattg
     1501 catctgcggc ggcaatcggg ctgattttga tttccggtaa attcctgctg tcgattttca
     1561 accacgatcc ggaagtaatt cagatcgggt atgtccgtct ggtcaccatt ttttccgcct
     1621 acgtgttcag catgctgtat gaagtgatgt ccggctatct ccgcggattt ggcatttcgc
     1681 tggtgccggc aattttgaca acgatcggtg tttgcggaat ccggttcgcg tggatctata
     1741 tcgtgttcca gaaaagtcag acatttcaga cgatcatgct ggtttatccg gtcagcctcg
     1801 cagcaaccgc agtattgatc ggcattgccc tgctggtcta tcgtccgtca cggcggctgg
     1861 aaaagaaagc tgtataaggg aaacactgca taaagcccgc ctgacggctt tgcacgcaat
     1921 ctgcttgcag a
//
LOCUS       DAWBGR010000294         1930 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_13254_length_1930_cov_3.096533, whole genome shotgun sequence.
ACCESSION   DAWBGR010000294 DAWBGR010000000
VERSION     DAWBGR010000294.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13254_length_1930_cov_3.096533"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1387..1396
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          481 a          456 c          392 g          591 t
ORIGIN      
        1 taaatccctt tggaactatt ttatcgcttt tttagtgccg aatcaacgtt agggtctggt
       61 tggcggttac gctacttcaa caatattatc cagaatcacg gataaagcaa caccgcacaa
      121 agaccacctg tcggctttgt acggtgttgt caaaattacg atgttttcgg acaagaggaa
      181 aggtctgctc tcagaggcag acctttcttt ttgtacaccg accccaaatg gagtagctcc
      241 atctggtcaa aaggtatttc ctgtacagcg tttccctaaa ggagaaagat tgcacccagc
      301 ggtcggattc agtattgcaa gtgccttgga cttatttcaa gttcttcaaa taggcttcaa
      361 acatatcttt ctctgcaaaa taaccttttt gaatcagcat tgttacaaat tcctgtacct
      421 ctgcattgtc gagccatgca cagtcgccgc caaatcggca gagttccagt tgctgtgttg
      481 tgccatcttc gtcatgatac aggacataga gtgtttgttc gccgtaatca gttattccaa
      541 agccccaggc tttcatttcg cagtccttgt attttgctgc attttttgtg aattccgaga
      601 tcttttgcaa aacctctgca tctgtgatgt acgattcctt ttcaccatct tctttgatgt
      661 ggttcatgtg gtcatcaacg gagagataca cgcaattgca caatggagct gaataccggt
      721 ttccgttggc ggtgattatt tgttcgtaat cttcgccatc ccagccgttt tctgacatga
      781 taattctata atcattgata aacaaaaccg gcgtatcatc atactggttt tgattcatca
      841 actcacgttt catcatgcaa aggtcaaacg cattcagtac gccgtcttca tacagatcgg
      901 ctgctttcca gttggaaagt gttgcatcgg gaacacccgt cagccatttc tggagcatga
      961 cagcgtcagc ggcattcaac ttcccgtctg cattgacatc gccctttaca ggctcagaaa
     1021 attcagattc gtctatttct tcgacggaca tatcttcacc gatcttataa taaccgacat
     1081 ctctgacgtt tttcgtctgc tctggatccc aaggtctgcc ttgagagatc gtaactttta
     1141 ctgtgccagc ggatactgct tcatatacat agacagcatg actttggctt ccaggtggaa
     1201 tcggctcatc ggaagaaata ttgtattctt ttacttcttt gatttccgcc gttccaagct
     1261 gctccagaat cacctcatca ccggtactat aattgatggt atcacaataa acgatatagt
     1321 tgccatgaac ggatacattt ccgtttttcg caatgaatgc ttcataatca tccattgtag
     1381 caggcannnn nnnnnngcag ccagtcgaat tcatcgacag attcaagagt gatcgtcttt
     1441 tcgtcatggt caatcgcctt gactctgatc gtgccgtcac cgatgtattt gctgtaatac
     1501 tgcggcagaa atacaggaag gacaaggtca ttgatatggt agtcatagca tagcagatga
     1561 gtatttctgt cgatcgtatc accgacggaa agcgttctgc cgtccagata agtcacgccc
     1621 tcaatatcgg gtgcgacata cataacaagt ccgtcaattc caacaatttc agcatcaaac
     1681 ggaagcacat aatcgccgct gttcagataa acaagatcat aatcaccctt tgtattgaca
     1741 acgttcagac agctccctgc tccgtctcct gtgtagtgca gggtatcgta ctttttcagt
     1801 tttgttccca gtgtaagttc ggaggtctga taggtcttgc ttgtctccac gtcatctgaa
     1861 acatctatgg taatggtctt atctgttcca atattggaat ggcttactgt aatggtcgct
     1921 gtgccgttgc
//
LOCUS       DAWBGR010000295         1913 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_13409_length_1913_cov_5.139397, whole genome shotgun sequence.
ACCESSION   DAWBGR010000295 DAWBGR010000000
VERSION     DAWBGR010000295.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13409_length_1913_cov_5.139397"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          432 a          529 c          474 g          478 t
ORIGIN      
        1 ctgtgcaatc tgccgaaaaa tctgactacg catcttacgc acaagcttta ttcagcgttt
       61 ccttaagcat attcgcataa aattttgccg aaaagacgtg catggcattc taatgtgcac
      121 acgctcctat tcaaggcagc atcatgtaaa gcttgttccg gaattccgca ggcacggtgc
      181 tgctcccaat actgcttttt gacagagaat cgaggtcatt gcatcatgcg tcaaccacca
      241 aaagaatctt cgcaatccct tagctatcat attgccctcg gaggaatcat tgctgcactc
      301 tgcatcgcct gcatgtttct cacaggcgtg ttccccatgt tctatctgat tttgcccatg
      361 ctggcgagtg ccttcatcta tatcatgaca ctggaaaccg gcggctactg gggctttctg
      421 acctatttgt ccgtgggact gctctccgtt tttgtcacgc cgaacaagga cgcttcactg
      481 gcattcctgt tctttttcgg ctattttccc ctgctgcgaa acggcatgga acggttccgc
      541 tggaagtttc tcgggttcct gctccggctg gcagtcttta atgccgcagt gctgacgttc
      601 ttctgggtgt ctgtctatgt actgggcatg aaagagctgc tggaatctct gggcgattac
      661 ggcaaatacg gcggctggat cctgctgggc attgcaaacg ttatgttttt ctgttacgat
      721 ttcagtatgg gaagctttcc cgcattgtat cgccgatacc tgaaaccacg aatctctccc
      781 ggtgcacatc gggagtcctg aaacaacact gaaaggagca tacacatgtc tgaaaaaaaa
      841 gaacgcattg ctttttttga caccaagcct tatgaccgtg tctggttcga ccagctgaac
      901 cggcactacc acatcaaata ctatgaacac aagctgacac cggattccgt gtcactggca
      961 cagggctgcc gtgcggtgat cccctttgtg aatgacacgc tggacaaaac cgttattgac
     1021 gggctgtgcg aagccggcgt ggaaatgatc gccctgcggt gtgccggcta caacaacgtt
     1081 gaccggcagg cagccgccgg acgcattgcc gtgacacgtg tccccggcta ttcgccttat
     1141 gccgtggcag aattcaccat ggggctgctg ctgacactaa accggaaaat tcacaaggca
     1201 tacttccgca cccgtgattt caacttttcc ctgaacggtc tggtgggatt cgatcttcac
     1261 ggcagaaccg tcggtgtgat cggcaccggc aagatcgggc agatctttat tcgcatctgc
     1321 cgcggcttcg gcatgaatgt agtggcatat gacccctatc ccctgccgga cgcagacttc
     1381 ccctatgttt cgctggaaga gctgcttcag cagagcgaca tcatctccct gcactgcccg
     1441 ctgacggaac agtccaggca cctcatcaat gccgacacca ttgcccagat gaaagacggt
     1501 gtgtatctgc tgaacacatc ccgcggcatg ctagtagaga gtgaagcact gctggacgct
     1561 ctgaaaagcg gcaagatcgc cggtgccggt ctggacgttt acgaggaaga aacggatttc
     1621 ttctttgagg accgctccga tatggtcaag cgggacaccc tgctctccct gctggtgtcc
     1681 atgcccaacg tggtactgac atcgcatcag gcgtttctca ccaaggaagc cctgcacaat
     1741 atcgcacagg tcacgctgga aaatctggac gcttacttca aggatggcac tgtgctgaat
     1801 ccggtgcagt gagaacagaa acacggttcg ccgtgttctt acatatagga aacactgaat
     1861 aaagcccgcc tgacggcttt gcacgcaatc tgcttgcaga ttttccgtca tat
//
LOCUS       DAWBGR010000296         1901 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_13513_length_1901_cov_4.416576, whole genome shotgun sequence.
ACCESSION   DAWBGR010000296 DAWBGR010000000
VERSION     DAWBGR010000296.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13513_length_1901_cov_4.416576"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          414 a          483 c          600 g          404 t
ORIGIN      
        1 cgaaaaattt gacgacgcat ctgtcgcaca atctttgtgc ggtattgcca taagagtctg
       61 ttcggcatag acagaaaagg agagatcaca tgctgactgt aaaagaagta tatgaagcca
      121 ttgacgcatt cgcaccgttt gcctcgcagg aatcgtggga caactccggc ttgctggtgg
      181 gcggtatggc acagcctgcc gaccgcattc tcaccacgct ggatatttcc gaagccattg
      241 tggcacaggc ggtggcggaa caggtgcagc tggtggtggc acatcaccct gtgatttttt
      301 cgccgctgaa aacgctgccg ccggacagtc cggtctacca gctggcggct cacggcattg
      361 cagcgatctg tgtgcacacg ccgctggaca tggcaccgct ggggctgaac acctttgcac
      421 ataccctttt ggaaaagccg ctggctctcc agggtacaca ggaagtgctg gagccgtcgt
      481 ggacggacgg cagaggcttc gggtggatcg acaccagtgc ccgcacatgg acaccgcaaa
      541 cgctggcaca gacgctgcaa acggcattcg gggaagtgcg gtattccgcc gcagagcgtt
      601 ccattcggaa aattgcctac tgttccggct cggctgcgtc catgctggaa ctggctgcgg
      661 caaagggctg tgacgctctg attaccggcg acatcaagca cgaccggtgg tatgcggcag
      721 agtttgccgg catcgctctg ttcgattgcg ggcactacgg cacggaacgc tttgccgccg
      781 gcattctggc ggaacagatc cgaaaggcgt tgcccgtggc acaggtgaca gagaacgccg
      841 gcagaaatcc ggctgagttt tgcaggggag tggaaaaagc atgagtgttt ggaagtgtcc
      901 ggtatgcggt tcgtttatgc agcaccgcgg gaaatcgtgg agctgtgcac agggacataa
      961 cttcgatatg gcgaaaagcg gctatgtgaa tctgctgccg gtgaaccgga agcacgccaa
     1021 gaatccgggg gacaacgccg tgatgatgcg tgcccgcaag aattttctgg acgcggggca
     1081 ttacgctctg ctgttacagg ctctggaaca ggcggcagtg cggtacggca gagccggcgg
     1141 cgtgctgtta gatgcgggct gcggcgaggg ctattatacc gagggcgttt cgaaagcact
     1201 ggcacaggcg gatctgcaaa tggaaaccta cggcgtggat atttccaaga gtgcggcaga
     1261 tgcggcggcg aaacgctgcc cgaatgccca tttcgcggtg gggagcgtgt atcacttgcc
     1321 ggtggcagac cggtgctgtg atatgctgat gacgattttt gccccctact gcggcgagga
     1381 gtaccaccgt gtgctccata agggcggcac tatgctgatg gtgattccgg ggcagcagca
     1441 tctgtggggg ctgaaagaag cgatctacga tgtgccctat cccaacgcag tgcgggacta
     1501 tgcactggac ggctttgaac tgatacaggc ggagtatgtg gacagctggc tgaagctgga
     1561 cgatccggca gatattcaga atctgtttca gatgacaccg tattactata agaccggacg
     1621 ggaggaacac gcccgactgg aacagctgga aagcctgtct acgcagatct ctttcgagat
     1681 cttgcagtat cgggcggtgg aaacggagca ggtgatgtga tagggagtgg gcattgtttg
     1741 tccgtaaatg ccgaaaattt gacgacgatt ttcgtgcttt ccggcatttt ttgtttctgc
     1801 gtttatccgg tttcatcgaa ttacacagcg tgtaaaagat tttacggaag aacccctcca
     1861 ccgcctaacg gcggtccccc tcgtactgag cggtcccctc t
//
LOCUS       DAWBGR010000297         1887 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_13665_length_1887_cov_3.204694, whole genome shotgun sequence.
ACCESSION   DAWBGR010000297 DAWBGR010000000
VERSION     DAWBGR010000297.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13665_length_1887_cov_3.204694"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          547 a          423 c          395 g          522 t
ORIGIN      
        1 aaagtacgca gttataaagt ttaacattca caccgttcac attccaaaag cctatctgca
       61 aaaaatgtat gattattttg tgcgtcggac agcagacaac gatttctgcc tggtgataaa
      121 cgaagacaaa tctcatgaaa ccattggaaa tatcatttct cacattgtcg cagaatacga
      181 aaaaaaggat gctatgtata tgcttgcagt gcagtcagga attcacatgc tgctgatcga
      241 aattgcaaga aacgttgaaa aatccggcat catgcagcgg gaaaagcata cagacatcag
      301 cctgtcatgt tatcatattc tggaatatat tgatacgcat tctgctgaac cgctggaggt
      361 acagcagctg gcagagatgt gccatatgag ctactccaat tttgcaagac tttttcggga
      421 aaattatggc cgttcctgta aggagtatat caagtatatt cgcttgaaca aagcacagga
      481 tctcttgctg aactctgatt atgatctgga ttatattgct caggaaaccg gattctttga
      541 ttgcagccac tttatccaaa cctataaaaa atggcgtgga atcacaccaa aacaagaacg
      601 aatgcagaac agagaagtcc tgtgaccata caaaaaaagc cgccttatat gcagtgagaa
      661 aagtgacacg ctttgccgca agccctctca acgtacaggt gtatgaaaaa agcggcagct
      721 tatcaaaaag ccaccgcttt tcaaagaaac aaaacctaaa aaccattgga acagcatgaa
      781 ttttcagaag cttcttttca tctgcggctt ggaacagagc cgcagagatt tattgcaaat
      841 gattcttccc attgggggag aaaatcattt tttactttga aaaatcccac gaagatagtt
      901 ataaaaatgc ggctgaacgc tagatgaatc atgtcctcca tcagaaacag aatgccagag
      961 atgttcaatt ccatttgcag acaagatatt gtgataaaca gaaggctgtg agccaactgt
     1021 accatctttc ccaccgccgg taatcatgat cagattcggc atctggtatg ccggtttcag
     1081 ctgattttcc tgcagctgac cggggtgcat actcaaatct gttccaggtg tcagtcctgg
     1141 tgcaggacag gcggcaccca catagccaaa caaatctgaa cgggtcaagc cgatgaacag
     1201 tgattctctc gcacccatgg aaaatcctgt aattgcagtg ttctcacgcc cggtcttcac
     1261 ggagaaattc ttttcgatat acggcatcag atcgtttgtc agatcatgga taaagttgtc
     1321 ataattcaat gaattctcca gatttaatcc atcgcatgcc tctttggttt tgctggtgta
     1381 gatatatgga aaaacaacga tcatcttttc cgcttcgcca tttgcattca aattgcccag
     1441 catggtacgg gcactgctca tagctgccaa agaatcctct gtttcccaat aaccatgcag
     1501 tgcatagagt actggatagg tttcgttttc attgtagtca gccggtaaaa gcacattcac
     1561 gttgacattt cgttcccgtg ttgtggaata gtactgatat tttttcagag taccgtaaga
     1621 aacgccagtt ttttcttccg tgattcctgc cggggcgtat tctgtcatca tattttttac
     1681 ctgttccata tatgcagttg gattcgtttc atcagaaccc gcactgcttt ttgagaatgc
     1741 tgtgattttt cccagcagat aatcctgaat cagctggaga tctgtaccgt ttacgctgcc
     1801 gctctggtct acgtctgctg ccagacttgc tgcattgccg gagaatgcac tggaaatgcc
     1861 ctgccgtgcc agtgccagat ccatgcc
//
LOCUS       DAWBGR010000298         1881 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_13716_length_1881_cov_4.754655, whole genome shotgun sequence.
ACCESSION   DAWBGR010000298 DAWBGR010000000
VERSION     DAWBGR010000298.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13716_length_1881_cov_4.754655"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          403 a          514 c          480 g          484 t
ORIGIN      
        1 gtcgccagac gctctttgtg cggtattgcc tcaatatttt cctgcaacca gatctgcaaa
       61 ggaagtggtt tttccgatgc cagcatatgc acaatacagc atcagataaa atgcatctgt
      121 ggaatcgaca agtccgttac cgtcaatatc gcatgccgca ggttttacat tttccggcac
      181 tgccacaccg gcggacttcg aagcaacgta atacatcaga taaaacacgt ctgtggtatc
      241 gatcgcaccg ctgccgtcgg tatctccggt cacaaacgga cgatacagca gagagatgtc
      301 acgagatacc tgcaaattgc cctccgcagt gacttccgca gtcggttctg aagcggacac
      361 cggttccgtc atctgctccg gaaacgaaac cgcatatgta tacgtgctgt ttccgctgac
      421 agatgcttcc atgtgggggt ctgcgatcag cacgctgtcg tcctcgaata tggctccgtc
      481 tgtatccggt tcgctgacgg aaacgtgata cacggagtta taaatgcagg tcagggacgg
      541 cgatgtataa gtatgcggaa attccgccgg attctgcggg cattcgcagt agtccaccag
      601 cagcacatac gctgttatgt cggcgtcttc tgccggcgac aggttcatat cataataggt
      661 tggtgtgccc tccggtgtga acttctgcac ggtcacatgc accggatagt cctgtacttc
      721 caccacaatt ctgccggtga tctggctgtc cggcaccggt ttcggcacat cggtagtcag
      781 ggctacactc tttgcacatt ccaccgtctt tttcacaggg gcatcggttt cttctgccga
      841 agcagcccac agagttccca gcatcgtgcc gcacagcacc gatgccgtca gtccggcaaa
      901 cagtttttgc aacatgatct tccctcctta atacggcata gtgaactgtc ccacacgcca
      961 cgagccgtcc tccagcacca gcgtgaacgt gtcctccttg gtagaggcag agccgtcttc
     1021 gggatcttca tagctggaag tgactgcaaa ggtcagctgg ctggaagtgc tggcggtgag
     1081 agccgttacc gcagacgata cataagaaat atcggctccg cggtcgccca ttctgccgta
     1141 cagcttatcc tcggacattt ccagcatgcc gtcaaagtcc gaagcgtgag cagacttggc
     1201 aaacacggca tagtacggtg cttcggcatc ttccagcgtg tcacagctgg tgatccgcac
     1261 gtagtcatcg gcaatggtgt cctcagtatc atacgtaaag ccgctgctgg agcagagata
     1321 ggtgtaatag gtctgtgcag ccttttcgta catctgctga gcctgtgcca cccattcgct
     1381 ttctgtcatg ccggtatccc cgactgtccc ctgagaggcg gctgcctctg cggtgactgc
     1441 ggcagtggtt tctgtggtgt cggcagtttc tccggcagct gtcgtgtcaa tggtttcggt
     1501 gtcctctgcc gcagtttccg tttctgccgg ctgagtttct gctgcttctt cggaagccgt
     1561 tgtttctgcg gtcatattta ccggtgtctg gctgtcgcca ttgccggaaa catctgtgca
     1621 tcctaccata agggctgcca gtgcacaaaa agcggtacag gcagcgagca tttttttctg
     1681 catgaagttc gaactccttt cttgcaggga cggtttcccc ttggggcaac caatataaaa
     1741 ttgcccccaa tctttcgact gtgtaacttc tattataaca catctttttc aaaaaggcaa
     1801 tgctttttct gcaattttcc ggagagaatt ctgcgttgtc aatagatgtg acccattgga
     1861 tgaaaagatg ccagagataa g
//
LOCUS       DAWBGR010000299         1864 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_13872_length_1864_cov_2.217247, whole genome shotgun sequence.
ACCESSION   DAWBGR010000299 DAWBGR010000000
VERSION     DAWBGR010000299.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13872_length_1864_cov_2.217247"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1333..1342
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          495 a          473 c          313 g          573 t
ORIGIN      
        1 gtctgtgtgg cgtaaaacaa cattccagtc attccacgga tactccggtt ctgtaccgta
       61 ttgttgcttg caccagctaa atacttcttg tcgtgtcatt catcccatca gtcctatatc
      121 attacttctt atctgtcaat atccattttc cttttctatt agacccctca cggacaatct
      181 tgccttcctg ctgcattttt gccatagtac gtttcactgt tgccgctgaa acagcaagta
      241 tctctccaag tgctttgtag gtaagctcag gagatttacg aattacttct tctaatcgag
      301 tttcgaggtc aattttgggg tcaacgggat catgattgag gtcattgagg tcacgattga
      361 gatcattgac cccattctga cctcgataaa gattgattcg gaacgcaact tccatgtcga
      421 taaattccgg ctcacgcaaa ccatattctt tcatagcctg cataagtttc ggaataccgc
      481 tgtcccatgc ttcaacaaga ttcatatatg caaaagcatt agcgagcgaa cgattcctga
      541 tttttgaaaa tccttctttc atcaaatcaa tcgtcacacc aggaagcaga ccacccggag
      601 acgtaatttc cagtcggtca tcataaattg ccacctgaat atttgaagcc tgaatgtaac
      661 tgcaattcat aaccgcattt acaatcaact cacggatact atccggcggc agctcataaa
      721 tatcctgacg atacacgcct tccaaccggc aacccatgcg gatatttcgc agaacaaatt
      781 ttaccgcttc gtctacttgg tcccaaagtg gtccttcgta attccggcga tctaaaaata
      841 ctgcacgggt tgttcctttg aacacgccgc actgtatcac ggaacgtaag ccgccttgcc
      901 ctgtcagata aacatacgca ttggttggat agatatttcc atcttcactt ttttttaaaa
      961 tgccccaact gatcagaaca ttttttgtca cgtcctttac aactgctctc tggagatcag
     1021 acttgccgtt cgcaatggca acctccttca tttgcctgca aagcgtgtca atatcttcat
     1081 ctgtcacctt caaatctctg cgaatcacag aatcaaaagc atgtccatcg cattcatagt
     1141 acaattctct ggacatctca cgatcagctg gacgagtagt gccagacaca cggatataca
     1201 caccattttc caaaccatcc gctttaatat aatatggttt ctgtcgtccg gctctaattt
     1261 cggcaacaat tactggtttt tcatttatgt tctgcaaata cacatctggg ataattgttg
     1321 gttcacagct atnnnnnnnn nntcgcattg gtaattgcgt cgatttcctg aaaaacaaca
     1381 tcttccgaaa ttccaactac ttctctggtt ttatcatcaa taccaaatac aatctgccca
     1441 cctttgccat tggcaaaagc aacaacagtt ttcatgtatt tgatactctt atccgggcgt
     1501 tgcaccttga actctacgtt ctgggattcc ccggcaagaa tttcttctat ggtcatattg
     1561 ccacctccca tatttaattt gcccgtatat cttcaaagta acacataggc attatcctca
     1621 taacatttta tcacaggcta tcctctttga acagataacc gttatattca aaaagagaac
     1681 tgccgataag tacgaggttt gtctctgtcc accgtcatgc ggtttggcat caacggatac
     1741 agagccacaa cttcgccctt gccgtttcag ataatctgtg cataggcatt gccccacaga
     1801 atattgctcc aattatccta agtatagcca tacttttttg actgtattca aggtactcat
     1861 attt
//
LOCUS       DAWBGR010000300         1840 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_14129_length_1840_cov_2.876190, whole genome shotgun sequence.
ACCESSION   DAWBGR010000300 DAWBGR010000000
VERSION     DAWBGR010000300.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14129_length_1840_cov_2.876190"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1612..1621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          410 a          466 c          407 g          547 t
ORIGIN      
        1 atgaaacaaa aagcggagtg aatacttgat gtattcacga gcatttttgt gaaagaagta
       61 ttgccttagc ctttttgcaa agcctgtcgc agtgctgtca agtcagttcc gtttatcaat
      121 gcatctttcg catagtcagc cgcctttgcc tgctgctctg tcagtgtcgt ttttcccagc
      181 aggtactttt gcagcaagat gatgtctgcc agatttactg cattgtccag attgacatct
      241 cccgccagta cagcatcaga atcggcatca ctgccgtcca gcggatattt tttcagatcc
      301 ggcagttcgt ccaacgtgat acagtactcg ctgttataga tctcttttag attctctgca
      361 gggttgttct gctcactggt aaggtagttc atataccacg ggcagaagta cagccacgaa
      421 gccttgtctt tcagcaggtt ttccaaagaa ggaatggtgt cgttttctgc cattgctacc
      481 attttctgtc cgtctgtact ctgcaccaga ctatagaatg tggaagaaat tgaacttagg
      541 tttggcttgc cgtctacggc attatactta tcgtatccta caatatctac cacatcatcg
      601 cccggatacc agtccggaga tgtggaatag gtgtagctgt tccagaccca aatcagattg
      661 tccaatccat agacgttagt cagctggtcg tagagaaggc ggtacagctt tttgcagggc
      721 tccggtcctt cggctcccca ccagaaccac gctccttcag cttcatgcag cggtcgccac
      781 agaaccggca catcggcatc tttcagcttt ttcagctgct ttgcaatttc cgcaatgtcc
      841 gccatcagca ccttattttc ccatgtgcct tcttccagtg ctctggaaat actgaatgtg
      901 gtatagttgg cactggcaga ttccacatag aaattgcagt ctgtactccc ctcctctgat
      961 ggcacgctcc agtgccacac aagggcagca atgcctttgt attcattcac ccactgaatg
     1021 gtacgttcgg ctgcgttatc gtcccatgtg gcattggaac gatagttcaa gaagtcgatt
     1081 ccgcgaatcg cacacatttt gccggtcgtc ttttccagat actcgaattc ctgttcgtta
     1141 tccttgatga aattggtcgg atctgcgtac aggttgtagt tatgcgagcc acagtactcc
     1201 tgctgtccgg aaatgatatg ctttccgtat tggtcgcaga gatactgata cagccgctta
     1261 gtggaatctg acgcattggg attggaaagc gttcttgttg gtgaaagatt tgtcaggctt
     1321 tcatctgccg gctgaatcgt caggtaatca aagaatgtat agccccaata ggcactcaat
     1381 tcaatggtgt tctcgccggc attgagcatc actacaccac cagaagtttc ctcgaatttc
     1441 aggttgtgag aaaagctgac ttctccctga tttacgccat tgacattcag gtattgcacc
     1501 tttttgtttt tgtcataggg ttcacagtag gaaatcttca tctcgtaaag accggcttct
     1561 tcgacggtaa cctttaggct gacagttgtc cccttttgat ccagatagga annnnnnnnn
     1621 ntcctcgccg ctgccgtcct cctcggtatt gcccttccag ccctcgttgt aaatttttcc
     1681 gccgctggtg cttccgtttt caaattcgta cttctgaaca gtgtcactgc tctttgcagc
     1741 tgccagaacc ggcattgcag aagatgcacc catggaaatt gtacatgctg ccgcaacgca
     1801 taccgcggcg actgaatatc gttttcttgt tttcaacgta
//
LOCUS       DAWBGR010000301         1805 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_14517_length_1805_cov_3.746286, whole genome shotgun sequence.
ACCESSION   DAWBGR010000301 DAWBGR010000000
VERSION     DAWBGR010000301.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14517_length_1805_cov_3.746286"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          449 a          476 c          402 g          478 t
ORIGIN      
        1 aacgagatta tttttaataa aaatgccgac aggcaggcgg tcgtgtcgga acggctaccc
       61 gcctgtcggc ggcgctcatt gctgccattg cctttacgaa ttgtctgcga gagatcttca
      121 tttttgttct cctccaaagt ccttattccg tagggtgatc ttcgtactgt gtcgcaccgg
      181 ccgggtcgat tttcctgctt ttgtcagctc ttacaaaagc agcgagagaa ttttgtgcat
      241 catcacatta cacagtttct tttctcttgt tgtgccatta ttatactatc tgtcagtagt
      301 ttcgtaaatg caaaaatgcg acttgttata tctcaatttg aaggaatact gcgaattatg
      361 ttgaagtcat aacgcatttt ggtatataat aagaaaaagc ggacaggagg cagcttgtat
      421 gcaagaatgt acaaaaagaa acgtatcaac ggaaacaaaa ttcaagttcc atgtgtttca
      481 ggatgagcga ttcgtcgatc tcaacttata ccaatatggc tgggagcaga cagagcctct
      541 gcactcctat ggtccctatg cccgcaacca ctatctgttc cactatgtca tctccggcaa
      601 agggcttctg cttgcaaacg aacaggaata caccatcgag ggcgggcatg gttttctgat
      661 cactccgggt gaggtcacca cctaccgtgc cgatgaacag gatccgtggg aatacacatg
      721 gatcgagttt gacggtctgc gtgcccacga agccctgaac cttgcaggca tcagcggaca
      781 gaacccggtg tactcgcctg cgagcaccaa agccggacag ctgctgcaag agcagatgct
      841 cttcctggta aatcacagtc aggacagccc catgcgtcag atcggctacg gctttctgtt
      901 tctggatcag cttgtacagt cctctgcctc ccatcaggca caaagtcccc gccgcctgcg
      961 ggacttctat atgaaagagg ctctgtcctt catagagcag cattacagcg aagatatttc
     1021 catcgaggac atctcctcct cctgcggact gaaccgcagc tatttcagca aggtgttccg
     1081 agataccatg ggcgaaagcc cacaggggtt cctgctgcac taccgcatgg cacgggcggc
     1141 acagctgctg actgaaagcc gccttcccat cagcaccatc agcaccatgg tcagctatcc
     1201 caatcagctt cacttttccc gtgcgttcaa aaacgtatat ggcatcagcc cacgggacta
     1261 tcgtgcaaag cattttgcac tataaaaatt ggtctcattc tccgctatac gcctttcgtt
     1321 tttccgacat ataaaaacac cccatttgtc agaccgaata ctgtcggaca aatggggtgc
     1381 tgttcattca ggggcgcacc ctgttttttg tttgccttta atcttctaac caatggctcc
     1441 cggttacgtt tttcaaatat ttctccgggt cgccgttcag aatcaaatcc gcatagccca
     1501 gtgggtcatt gtagataagg tagtccagtt ccgtctgctg tgccatggtc acatccagtg
     1561 catcctcaac cccggtacag tcaatggaaa tttttctccc atcccggagc agcagttcca
     1621 cgcatccggt gtctatgttg aatttgcagg ttctttcatc gtacttcatc aaatgtacat
     1681 ccacaactat gattcatggc agggatttgg acaatcgaac agacatcacc ttaatataga
     1741 aagaggtttc cttatgaaca aactgctttc ctgccgctac aacatggaca ccaaccgggt
     1801 ggaag
//
LOCUS       DAWBGR010000302         1792 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_14660_length_1792_cov_2.922855, whole genome shotgun sequence.
ACCESSION   DAWBGR010000302 DAWBGR010000000
VERSION     DAWBGR010000302.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14660_length_1792_cov_2.922855"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          487 a          411 c          340 g          554 t
ORIGIN      
        1 cgtttttctg tcacacgaat ggcaaacggt tcaatttcag aagaccagac aggcaaaatg
       61 cctgccagca gtccggcaag cggaaaaccg ccgctaccgt caaagaggct gccaagggtg
      121 agcgatctat tcattagtca cctccagatt atttcctgct ttatcgcatg ataaaagaaa
      181 cgccctgcat actgataatt ctgcaaagcg tttacatttc tctataactt tacttcgtat
      241 ccatattggt gcggcaggaa cgttgttata tcttccatat tcgccaaaca tacactccat
      301 accaacattt cttgcctgaa ctgcctcttg aaatgtatcg tagtatccaa gatgaatatc
      361 cagctgactg attttgattc ttgcacgata tttctttctg ggtggataat aacttactcc
      421 gcttacaccc gatgtgttat ttttttgaag cggttgattt atttgatttt gctgatgtgt
      481 acagaaacgg acattgcatc ttcgattatc caatgtatca agattgatat gatccagctc
      541 cattcctttt cttgtactaa aaagcacttg atgcagcggt cgaccatggc agtcaacgat
      601 atatatttgc ctacctttcc tgcttttata agagacatac catttgatat cttttattct
      661 actgaataaa tcagcatcaa acatgaatat cgtcccatcg gaaagatgac catagccaat
      721 tataccatca tcagaaaatg tgtaattcac attgccgata tcactcacgt cctctctgag
      781 cgtcattcac attgctgtct tgaatattca gttcgtctgc tgaaaactca tgtacttctg
      841 aacacgggta ctttactccg ttgcgcagaa catacacgcc atcagctgaa ccaacagcag
      901 caatgtatct tctgatgatt gctgttgcat atttgggatc aagttcttgt gtgtaacaga
      961 ttctgttggt ctgttctgat gcaatgagtg tagagccgct gccgccgaaa agatcaagaa
     1021 tgattccgtt ttcctgtgat gacatacgaa tcggatatgc aatcaatgga agagttttca
     1081 ttgtaggatg tagctttgac tttttcggcc tgtcgaattc ccatacagta gtctgcttgc
     1141 ggtcaccgta gaatttgtgc tttgcagtat ccttgaaagc ataaattact ggttcgtgcc
     1201 gcatttggaa atccattctg ccgataacaa gtgtatcttt tacccagata caagttgtag
     1261 aatagtggaa tcccgcatta actgttgctt tataaaaatt acatttttct gcatctgaat
     1321 ggaaacagta aaatgctccg ccgtctgcga gagatgtata tgcgtttttg aacgcatcca
     1381 agagaaactg atagaacttc tcactgtcag accatttgtc attcataatt gtcatacctg
     1441 taccgccgga atatgcacaa ttatacggag gatctgtaat acaggcattt gctttctgac
     1501 cgtccatcag caaggcaact tcatcaggtt tggtggaatc tccgcagcga agtctgtgtc
     1561 tgccgagaag ccagatgtca ccattttcaa caaatggttc aaactctgcc gccttatcta
     1621 catcaaaatc atcatctttt acatcttcat ctgatgcaaa taagtccgca agttcctttt
     1681 catcaaatcc agtcatggaa aggtcgaatc cgagctcctg tagttcctgc atttcaacgg
     1741 acagcagttc ttcgtcccag cctgcatcca atgccatccg gttgtcagca ag
//
LOCUS       DAWBGR010000303         1787 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_14721_length_1787_cov_4.196305, whole genome shotgun sequence.
ACCESSION   DAWBGR010000303 DAWBGR010000000
VERSION     DAWBGR010000303.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14721_length_1787_cov_4.196305"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          495 a          382 c          457 g          453 t
ORIGIN      
        1 acagactctc atatttcaca aaaatgctcg tgaatacatc aagtattcac tccgccgcaa
       61 tttgtaaaag taaccagttg tttcttttgt gaacagctgg ttatttttgt atcttttcga
      121 aaccgcaact gtttttgacg attcaaatgc tatttttgac aatttctaaa aagtgaagaa
      181 aaagtgtgct ataatgatac acaaggatta caggaaacgc aggagaatga tattcttcgg
      241 ttctgcgatc cgcttctgaa ataccgaagg gtgcgaaaaa acgggaaagg gggaatgctt
      301 tctgccggaa tgaaagtcgc ttctgaatcc ggcaggagca ggaggagaag tatccaaagg
      361 catttcaaac cacttatact atggcgtatc acacgccgcg gaatgtgtcg aagatgacac
      421 caacagaaag gaaaggtttc aaaatgaaag caaaaatgat gaatgtttac ggtgtgtttg
      481 cagcagcctt tgccgcagca gcaatgatga tgaccacgct ggtggcaaac tccacctgca
      541 tctacttctc ctatcaggac gagctgccgg agcgtgcaag aaagctgcgg aagttctgat
      601 gtttgcgaag ctgtcagata ccattgcacg ttcttttgtg gcacgcggaa ccatttcaga
      661 agaacgtttt tccatatgcc gctatgggat caagcaactg ttttccgtct gtctgaacct
      721 gctcacaacg ctttgtatcg gcatggtttt cggtctggtg tgggaaagtg tcttgtttac
      781 ggcggcgtat atcccgcttc gcagctttgc cggcggattc cacgcgaaaa cgccggtacg
      841 gtgctactgg tattcggcgg caatgatcgc gatcgtgctg gcactgctgc ggtttgtgcc
      901 gtttcccctg tggacggcgg ttccggtata tatgatttcc agcatactgt tgtggctgct
      961 tgcaccggtg gaaacttcgc ataagcctct ggacgagctg gaacagcggg tgtaccggca
     1021 tcgtgcccgt ctgatctggt gcgtggaaag tctggtcatg atcgggttat ttttcctgca
     1081 attggagcag gcaggaaatt gtattttgct gtctatggca gcgttaagcg tgatgctggc
     1141 ggcgggaaaa ttccagctgc atcacacaca tcaggcataa gggaacactg cataaaaaca
     1201 gaaaaacaaa agaaaaacaa aagaaaaaca aaagaaagaa acaaaacgag tgagaaccga
     1261 tccgttttcg ccgcgaaaaa aatatccgga gagaaaaata tatcggactg cggcatgcac
     1321 tgcgtttgga gacaacaaaa cacgcaaatg cggaaaatcg gaatacatga ggtgcagcct
     1381 tgtacagtgc tgccggaaaa gcgttcggaa gccagaagtc ttccggacgc ttttctgcac
     1441 cttttttgga aaagtgaata tactggggac agaggacacg gaatgtgtgc ctgtgacaaa
     1501 ttacatgtat gtttgaaagg agtttcatat gaaaatcgtt gttgtcaaga gcccgaagct
     1561 gttgtccagt ctgtttcgca tgattttcag gatccagaaa gaaacgccgg cggaagaaca
     1621 gtaattttgc cttagacctg tgagcagaag catgctgaca ggtctatttt tctgtcctgc
     1681 ttcatatggt agtagcacaa tatcgccgca agtttagggc aacaccgcac aaagagcgtc
     1741 tggcgacttt gcacgccatc tgcgtgcaga ttttccgtca tattgcc
//
LOCUS       DAWBGR010000304         1786 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_14728_length_1786_cov_3.035817, whole genome shotgun sequence.
ACCESSION   DAWBGR010000304 DAWBGR010000000
VERSION     DAWBGR010000304.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14728_length_1786_cov_3.035817"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          538 a          354 c          474 g          420 t
ORIGIN      
        1 gagaataaga acactgaaaa agagaggcta tgcaactatg atattgttat ctgcacaaaa
       61 tattgccaag acatatatgg aacgaaaagt actggacgac gtttccttct ttttgaatga
      121 gggtgataag gtggggatta tcggcatcaa tggtaccgga aagtctacac ttttgcgaat
      181 ccttgccgga gccgaagagc cggacagcgg taatgtcatt cgcactaacg gaatccgcat
      241 ctcatacctg ccgcaaattc cggagtttga accgcacggc agcgtattgg aacaagtcat
      301 gctgcatttg ccggcagatc ttaaagaggc aaagacgttt gaagcgaagt cgattttggg
      361 aaaattgggc attacagacg aaaccagaga tattagtaca ttgtccggcg gcgaaaagcg
      421 gcgtgccggc attgctgctg ctctgattca gcccagcgat gttttgctgc tggacgaacc
      481 gaccaaccat attgacaacg aaacggttcg attgctggaa gaacagttat tgaaatatcg
      541 gggtgccatt gttatggtaa cccacgaccg atactttcta aaccgcatta cccgaaaaat
      601 cgttgaagta gacagaggaa agatttttga atatgaggga aattacagta cgtatctgga
      661 acagaaagca cagcgggaag ttgacgcaga agcaaaggaa cgaaaaaatc gttctttgta
      721 tcggcaggag ttggaatgga ttcgccgcgg cgttcgtgca agaggcacca aatctaaaga
      781 tcggatcgca cggtttcacg aattggaaaa tcgtgagaag ccagcagaag cagaaaaaat
      841 gcagctgcaa tcggtttcct ctcgactggg aaagaaaact gtggaaattc atgcaatcac
      901 caaggctgtg aatggcagaa tgctgattca tgatttttcc taccaacttc cacgcgatgc
      961 cagaatcggc attgttgggc aaaatggtgc cggaaagtcc acgctgatca agatgctctg
     1021 cggacaaatc tcgccggaca atggttctat tgaactcggt gatacagtac gcattggcta
     1081 tttttctcaa gagtgtgaaa gcatggatcc caaccagcga gtcattgatt acattcgggg
     1141 aactgcagag cgaattcaaa caccggacgg cacggttact gcggcgacaa tgctggagcg
     1201 attccttttt acttcagaat tacagtggaa tcgaattgaa aagttgtctg gcggtgaacg
     1261 gaaacggctt tatcttttga aagtgctgat gactgcccca aatgtgttgc tgctggacga
     1321 gccgaccaat gatctggata ttgcaacact tactattttg gaggactatc tccgcagttt
     1381 tcaaggggct gttcttgcga tttctcatga ccggtatttc ttagataaaa tggcttcgga
     1441 aatctgggaa ctgacgggga acggtgaggt tcgccgctac aacggcaatt atcaggatta
     1501 catggaaaag gctctgccag aaaatgcgac agaaaagccc aaaaagcaaa cggaaaagaa
     1561 agaacgcgtt ttcaacggca ggaaaaagct gaagttttct ttcaaggagc agcgggaata
     1621 cgataccatt gatgatgaaa ttgccgattt ggaagaacag attcggctga cagaaacgga
     1681 gattactgcg agtgtgtcgg attatgtgaa gctgcaggaa ttgtctgacc ggaaagaaga
     1741 actggaagtg cttctggcag agaaaatgga gcgttgggtg tatttg
//
LOCUS       DAWBGR010000305         1780 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_14795_length_1780_cov_5.851594, whole genome shotgun sequence.
ACCESSION   DAWBGR010000305 DAWBGR010000000
VERSION     DAWBGR010000305.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14795_length_1780_cov_5.851594"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          441 a          480 c          415 g          444 t
ORIGIN      
        1 gcaagcagat ggcgtgcaaa gtcgccagac gctctttgtg cggtattgcc ttaacaacta
       61 taaaaaaacg ccgccagatc tgttgcactg gcagcgttct gttcgtatca cgaatttacc
      121 gttcttcgca aatcagcttg attgcagtgc ggtcttcgcc atcaatctga atattggcaa
      181 acgccggaat gcagatcaga tccacaccga tcggagcaag atagccgcgg gcaatggcaa
      241 ttgcttttac tgcctgattt gccgcaccag caccgactgc ctgtacctct accatctttt
      301 tttcacgaat cacgccggca attgcaccgg caaccgaatt cggagatgag tgcgatgata
      361 ctttcaatac ttccatgata aaagcctccc ttagtacaac caatgattcc atgttgtatt
      421 cgatattccg cctgtaaaaa aacgacggca aatctgtgta aaaccgatca gttctgctcc
      481 ggcttccggc actcggttta ggcagcaccg cacaaaattt ataccgtgct gcccttacac
      541 accctcatta tacaacaaag cctttgaaat tgcaagtact tcccgaaaaa tttgtgcatt
      601 atcgaacaat cagccgttcg attttcgtgc atttgccaca cttttcatca aatgtgacga
      661 ttacgccgtt caaaaagcag ggatcgccgc cttctgtaaa acgtgtagga tagtggaaac
      721 ggaaccggtc cattgccggc tgcaccgcaa cgcccagaat cgattcctcc ggtccggtca
      781 tgcccacgtc ggtgatatag ccggtatgtt cggacaaaat ggtttcgtcg gcagtctgca
      841 catgagtatg cgttcccacc actgccgtta cacggcctgc cagaaaatag cccattgctt
      901 ttttctctgc cgtcgcctcg gcatggaaat ccaccagaat attcggagtg tcgatctcct
      961 ccagaagctg ttccgcaatg gtaaacggat tatccagcgg atccagatac gccgtaccgg
     1021 acaggttcag caccgccacc tgacagggac cgaaatcaat ggtgcatacc ccgtgacccg
     1081 ggcagccctc cggatagttc gccggacgga gcaccaccgg attttcgtag atcgccattg
     1141 cctcccgccg cttgaagcag tgattgcctg tggtgatgac atctgccccc gcagaaaaga
     1201 tctgttccat ggagtgcttg gtgatgccgt ttccctgtgc ggaattttcg ccgttgacaa
     1261 caatcatatc gattttgtgt gtgcgtttga ttgccggaag atgttcctgc aaaaagcggc
     1321 tgcccgctgc accgacaaca tctccgatgt gtaatagttt catgtatggg ttccctttct
     1381 aagagcgtgc tggcatgaaa gaaacactgc ataaagcttg tgcagatgcg tcgtcagatt
     1441 ttttcggcag attgcgtgca aagccgtcag gcgggcttta tgcagtgttt cctgaattat
     1501 agcatgtatt ccgcggctgt gtcaagcagt ttttgtcgaa caccctgacc agacacaaaa
     1561 aataaagcac acaagagcgt tccttgtgtg ctttacaatg gaagagataa tacaatgcaa
     1621 atggagcgga ttacgaggct cgaactcgct acctccacct tggcaaggtg gcgctctacc
     1681 agatgagcta aatccgcatc cgaaacgtgt tagtcaacac gttccatggt gcctccgatc
     1741 ggaatcgaac caatgacacg gggattttca gtcccctgct
//
LOCUS       DAWBGR010000306         1778 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_14821_length_1778_cov_5.068485, whole genome shotgun sequence.
ACCESSION   DAWBGR010000306 DAWBGR010000000
VERSION     DAWBGR010000306.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14821_length_1778_cov_5.068485"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          445 a          495 c          462 g          376 t
ORIGIN      
        1 ctccgctttt tgtttcatct gacgaaaaat ttgacgacgc atctgtcgca caatttttgt
       61 gcggtattgc cttagggaaa cactgcataa agcccgcctg tggctttgca cagtgttccc
      121 ttcgcaaaat cagacagccc gatgcagatc tggatacggg ctgaccaaaa gaaccgcctg
      181 acgtgcgaat atcctgctgt cgtcagtgcg tgaaatcgaa tttggcggcg gaactgccgc
      241 gataaaggat aggaagcgtg aatgttatgg aaaacatgga aaacaatttt gacgtgatca
      301 ttgccggttg cggtgctgcc ggactgtacg gtgccatcaa tctgccgtca gactgccgga
      361 tcctgctgct gtgcaaacag gaactgaccc tgtgcaactc tgcactggct cagggcggga
      421 tcgccggtgt gtacaagtcg ccggaggatt ccattgaact gcacgagcat gacaccatgg
      481 ttgccggcgg gtttaccaac aatccggaaa cggtgcacat tctgaccaca catgccgcac
      541 acgagattca ggcgattctg gatttcggcg tggactttga ccggacaccg gacggtgaac
      601 tccaccggac actggagggc ggtcacagcc gccaccgcat tttccaccac aaagatgcca
      661 ccggtgcgga gatcctggac aagctgatca aaaaggtaaa gaccctgccc aatgtgacca
      721 ttctggaaca cgccatgatg acgggcatgc aaaagaccga caccggcttt tctgtcaatg
      781 tgatgcaggg cggctcctgc accgtgtaca gtagccactt tatgatactg gctaccggcg
      841 gcatcggacg ggtctaccgg ttcaccacca attccaagat cgctaccggc gacggcattg
      901 catttgccta tgaaaacggt gcggagattc ggaacctgca tctgatccag tttcacccca
      961 cagcattcaa cgaccaccac accagagagt gcttcctgat ctcggaggct gtccgcggcg
     1021 agggggcata tctgctgaac tgcaacaagg aacgcttcat ggaccggtat gacgaacggc
     1081 tggagctggc tccccgtgat gtggtttccc atgccattat tctggaaagc cgcagaaccc
     1141 attctgacaa cttctatctg gacatttcct ataaggactc ggagttcatc aagaaccggt
     1201 tccccatgat ctacgaaaag gttctggaac agggctacga catgaccaag gagccgattc
     1261 cgatcttccc ctgccagcac tacctcatgg gcggcattca ggtggacgac aattccgaaa
     1321 ccacagttcc caatctgtac gctgccggcg aatgctccca taccggtgta cacggcaaca
     1381 accgccttgc cagcaattct ctgctggagg ctctggtgtt ctccagacag gcggcactgg
     1441 acattgccaa gaagctgccg gacgctccca agacctttac cgccgcagaa ttccctgcca
     1501 agccgaaccc gcagaagatt ccgaaaggac tgcgtaccga aattcggcag atcatgcagg
     1561 acaactattt tgttattccc aacaaggaaa agctgccgga gggattccgg agaatcaccg
     1621 agatccgcga ccagctgctg aacggcaact atattgtaga tgccgactat gtagaagcaa
     1681 agtcgctggc aacggtggca tatattattc tgaaagaagt actttaagga aacgctgaat
     1741 aaagcccgcc tgacggcttt gtacgcaatc tgcttgca
//
LOCUS       DAWBGR010000307         1772 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_14887_length_1772_cov_4.041351, whole genome shotgun sequence.
ACCESSION   DAWBGR010000307 DAWBGR010000000
VERSION     DAWBGR010000307.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14887_length_1772_cov_4.041351"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          481 a          412 c          475 g          404 t
ORIGIN      
        1 ggcatggatc tggcactggc acggcagggc atttccagtg cattctccgg caatgtagca
       61 agtctggcag cagacgtgga ccagagcggt agcgtaaacg gtacagacct ccagctgatt
      121 caggattatc tgctgggaaa aatcacagaa tttccgattg cagaaaaaat agtggataca
      181 gcagcaatgg aaaagctgtt tgccggaatt acgccaactg ccagctataa agtagaaggg
      241 gaaaataacc cgctgtttac ccagcgattc ggagctgacc ctggtgtcat ggagtataac
      301 ggcagagtgt atgtctacac caccaacgat gtcattgagt atgacagcaa tggaaatgtc
      361 accgaaaata cctacgcaca agtcaacaag atcaactgta tctcctccga cgacatggta
      421 aactggaccg atcatggtgc gattccggtg gcaggtacag agggaatcgc caaatgggca
      481 acttgttcct gggcaccctg tgccgcacac aagaccatca atggcaagga aaagtttttc
      541 ctctatttct gtaacggcgg aaatggtgtc agcgtgctga ccgcagacag ccccactggt
      601 ccgtggagcg atccgctggg aaaggcactg attaccagag ccacacccaa ttgcggtgat
      661 ataacatggc tgtttgatcc ggcagttatg gtagacgatg acggaaccgg ttatctctgc
      721 ttcggcggcg gtgtgccgga cggaaaagat gctatgccgg gaacttcccg cgtggtgaaa
      781 ctgggcgagg atatgatcag tcttgccgaa acgccggtga ccattgaagc accgtatctg
      841 ttcgaggatt ccggtatcaa taaaattggt gacacctact attacaccta ctgctccaac
      901 tggaatactt ccggaaattc ctacggaatg accagcggtg ctattgagta tatgactgcc
      961 agcaaccccc ttggtccgta tacttacggc ggagaactgt tcccgaatca ggggaagttt
     1021 ttcggactgt acggcaacaa tcatcattcc atctgtgcgg taaatggaca gctgtacttg
     1081 ttctatcaca accgcagcgt agaaaaggca atggggatcg agggcaatta tcgcagtccg
     1141 caggtggatc agatcacaat gaccggtacg aaaatcaata cggttaccgg cacaatgaaa
     1201 ggaatcgcac agcagaagtc tgtgaatccc tacgtcaaga atccggcgga gatgatgtcc
     1261 gaccaagccg gcatcaatgt ccgtggactg ggtgatacag ttgtcacgga aattgacaag
     1321 ggcgactgga tcaaggtcag cggtgttgat ttttccaagg gtgcatcgca gatcgttctg
     1381 acagcatctt caaagttcgg ctgtgcagtg aagatctgca ctggttctcc tacgggaaag
     1441 gctgtggggt atgctgagat tccggcaggc ggtaagctgt ccgaagtttc ggctgcggtg
     1501 cagggactta ccggaaatca ggatttgtac tttgtattca gcggtcaggc agaaatggat
     1561 tgctggatcg caaagtaaag aaaacaccag tttccctaaa aaaacaaaac gctgaacgat
     1621 cttgtgcata agaccgttca gcgttttctt attgattatg tccgcgtaaa aatgaataca
     1681 tgtaaggcaa cactttcagt gttttcctca gttttggctg aggcaatacc gcacaaagag
     1741 cgtctggcga ctttgcacgc catctgcttg ca
//
LOCUS       DAWBGR010000308         1751 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_15119_length_1751_cov_5.681604, whole genome shotgun sequence.
ACCESSION   DAWBGR010000308 DAWBGR010000000
VERSION     DAWBGR010000308.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15119_length_1751_cov_5.681604"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          427 a          441 c          526 g          357 t
ORIGIN      
        1 aatatgacgg aaaatctgca agcagattgc gtgcaaagcc gtcaggcggg ctttatgtag
       61 tgtttcctga aaatactacc gaaaaaagga gcaaatgcca tgctatattc gtatacactg
      121 gaaacaccgg cagaaaatct ctgcccgatc acggcacagg tacagggagc cgtagcggac
      181 agcggcatca cagacggcat ctgtgtcgtt tactgccccc ataccaccgc cggtatcacc
      241 attaatgaaa acgccgaccc cgatgtggta acagatctgc tgctggcata tcggaaggcg
      301 tttccggaca gaccggaatt tcgccatatg gagggcaatt cctctgccca tgcccgtgct
      361 tcggcaatgg gcagcagcgt gaccattccc gtgcggaacg gcagactggt gctgggcaca
      421 tggcagggcg tgtacttctg cgaattcgac ggtccacgcc gccggacgtt ttatgtcagc
      481 gtcattgccg gatagaaagg ggcaggtatc atggaaacgg aaaaaatcaa acaattgcag
      541 caatgggtgg atcagtcgga tcgtatcgtg ttttttggcg gagccggcgt ttctaccgaa
      601 agcgggattc cggacttccg cagtcaggac gggctgtatc atcagcagta tgacgtgccg
      661 ccggaaacga ttctgagcca cacctatttt gaacggcata ccgcggagtt ttaccgcttt
      721 taccggaaca aaatgatctg tctgacggcg aagcccaatg cggcacacaa gaagctggcg
      781 gagctggaac aggcaggaaa actgctggca gtagttaccc agaacattga cgggctgcac
      841 cagctggcag gcagcaggag tgtgctggaa cttcatggca gtgtgcaccg gaatatctgt
      901 caggtgtgcg gtgccgtcta tgatgccgag tggatcatga tgaccaccgg tgtgccacac
      961 tgcgacaaat gcggcggcag agtgaagccg gacgtggtgc tctatgagga atctctgccg
     1021 gaggacacgc tgacacgctc tgtgaatgcc attgccaacg cagacttgct gctggtgggc
     1081 ggcacttcgc tgacggttta tccggcggca ggactgctgc ggtatttccg cggggaacat
     1141 ctggtactgc tgaaccgtga tgcaacgccc atggacgaac aggcagacct gtgcattcgg
     1201 gaacccatcg gcaaggtgct ggaacaaatt caagtgaggg atcaggcatg aaaatgttgg
     1261 tacagcgtgt aaaacacgca aaagtcaccg tagacggcaa tgtcaccggt gcaatcgaac
     1321 aggggtatct ggtgctgctg ggcattgcac cggaggatac cacggagatc atggaaaaaa
     1381 tggtggacaa gctgctgcgg ctgcggatct tctcggacga aaacgataag atcaacctct
     1441 cgctgcaaga tgtcggcggc agtctgctgc tggtgtccca gtttactcta tatgcggact
     1501 gccgcaaggg gaaccgcccc agttttgtgc acgcggcacc gccggcacag gcggaagccc
     1561 tttatgaagc atgcaaggcg tactgtcggg aacgggtgtc tgtggtggaa tccggcatat
     1621 tcggggcaga tatgcaggtg gaattgtgca acgacggacc gtttaccgtt ctgctggatt
     1681 ccgcggaaat catcaagaac taaggcaata ccgcacaaag agcgtctggc gactttgtac
     1741 gccatctgct t
//
LOCUS       DAWBGR010000309         1729 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_15353_length_1729_cov_2.663680, whole genome shotgun sequence.
ACCESSION   DAWBGR010000309 DAWBGR010000000
VERSION     DAWBGR010000309.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15353_length_1729_cov_2.663680"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          567 a          311 c          381 g          470 t
ORIGIN      
        1 tggaaaatga tgcactatac gaggagcacc gtggtcaaat gatgctgggc ggcgagggcg
       61 aatataaccc gattcaggtt tccattggac agttttacgg catcgagatc aacgattttg
      121 cggtgacggt tgcaaaaaca gccctgtgga tcgcagaaag ccagatgatg caggaaacag
      181 aaaagctgat gcagatcaat ctgaatttcc tgccgctgaa atcctatgca aatatcgtag
      241 aaggcaatgc tctgcggatt gactgggaaa gcgttgtgcc gaaagaaaag ctgaattata
      301 tcatgggaaa tccgccgttt gtgggtgcaa gattgatgaa caccatacaa aaggatgatg
      361 ttttatcgat atttgatggt gttaaaaaca acggaaatct tgattatgtt tcctgttggt
      421 ataaaaaagc ggcggatatg atgacaggaa cagaaattag aacggctctt gtttctacga
      481 attccattac tcagggagaa caagttgcaa tcctttggaa aaacctgttt gagcatgggg
      541 ttcatattga ttttgcatat cgtactttcc gttgggatag tgaagcaagt ttgaaagctc
      601 atgtgcattg tgttattgtt ggatttagca aagcatcaaa tcaaaaacca aagcggattt
      661 ttgatggtgg ctctgtaaag attgcgaaaa atataaatgg ttatcttatt gaagcagata
      721 atattgttat tgaaagtaga actaatccta tatgtaaagt aaatacaatt agaatgggca
      781 atcagcctat tgacgatgga aattatatct tcacagaaga agaaatggca gaattcatta
      841 gaatagagcc taaatcaaaa ccgtattttc atcttttcta tgggtcgaga gaatttatta
      901 accgtattcc tcgatactgc ttatggcttg gagattgttc gccaaaagaa ctgcattcta
      961 tgccagaatg tatgaaaaga attgaaaatg tccgtcaatt tcgcttatca agtaaaagag
     1021 caagcacgct tagactcgct gatacaccaa caagatttca aactgaaaat atgcccacca
     1081 caaattacat tgtaattcca gaagtatcct cacagaatcg tcgatatgtg ccaatgggct
     1141 ttatgacacc ggatattatg tgcagcaata aagtgagact tatacctaat gcgactttat
     1201 atcatttcgg tgtcctgaca tctaatgttc atatggcgtg gatgagaaca gtgtgtggaa
     1261 gattgaaaag cgactatgat tattctatca aaattgtcta caacaacttc ccttggtgca
     1321 atccaacacc tgaacaaaaa gcaaaaatcg agaaaaccgc tcaggcaatc ctggacgcaa
     1381 gagcacttta ccccgactgc tcccttgccg atctctacga tgaaaccacc atgccgccgg
     1441 aactccgcaa ggcacatcag gcaaacgatt tcgccgtgat ggctgcctac ggctttgacc
     1501 ggaaaatcac agagagcgag tgcgtggcgg agttgatgaa acggtatcag gagttggtga
     1561 aggtaaaaaa gtagaatatt tatctaattt aaaaggaaaa tgatacaatc aataaaagga
     1621 gtcaattttg gatatatcga cttttatttc taactttaag aatcaccctg ttttgtttgt
     1681 aggtactggc atgagtctca gatacttaga aaactcatac acatgggat
//
LOCUS       DAWBGR010000310         1717 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_15495_length_1717_cov_3.070999, whole genome shotgun sequence.
ACCESSION   DAWBGR010000310 DAWBGR010000000
VERSION     DAWBGR010000310.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15495_length_1717_cov_3.070999"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          436 a          416 c          390 g          475 t
ORIGIN      
        1 gtgcaaagtc gccagacgct ctttgtgcgg tattgcctta tgtggatttt gttgtcatgg
       61 tcttctgatg gaactgacac tttttcaaca acatatctgc tgtttccgca ctggggctca
      121 atactttcaa ctcctccaga tagtgcagat atgccagaat cgcagaagaa atggattttt
      181 ctttcagcgt gatcaaaaag aaattctcgt ggaagttcat gaccagaacg attgccccgt
      241 cattccagat gtccagaccg tgaatattcg catgatccat gaacgaatat ttcagcatct
      301 gaaaacgcca gcggtcactg ttctgtttca gaatactgga cagcatctca taccgccgtt
      361 ccttggagat cggtgtggag atcgattcgg aatattcgta gtacaggtcg ctctggaaaa
      421 actcctgcat gccctgctcc gaaaaaatga tgtgttcgca tttgacacgt gtcatatagt
      481 ctaccagata ggtatagcag gtatttgcgt tataaccgga ttcctcctct gacagataca
      541 tggtctgcaa gtcccgctcc gaaatcccgc tggaaatgca gggatgcttg gcgagaatat
      601 agagttcctc tccctgccgt tcgttgacca tatttcgtgt ctggttgatt gcctgaaaaa
      661 attccggcaa gctgaaaaac ttgtgacaga aaggattgct gcggtcaaac agaaaggcgt
      721 actgcttctc gtacagagaa actgtcgccg gctggcagaa ccacattgcc ttggtgcagt
      781 cccgtgaaat tgtcagggca ccccgttcgg tcacaataaa ataggggaac aaatgctgct
      841 ccaacagctc cggcaaagct ccataagtac atcgaatttc catacgcccc gcacagatga
      901 ctggcagagc tttcgaaaat acgtccagat ttgccgccga aatgccgctg tcagaaggtt
      961 ccagccagaa cagccactgc atggacaccg tatcgccgca ggaagccacg catcgtccta
     1021 agatctccaa caggtcctga tcctccggct gtatggcgat ccgcacggaa gcggtgttca
     1081 ggcaaagccg ctgcaccgcc gcaaggactg ccattttgtt ggtgtacagg tggccgtttt
     1141 ccgcagaaac cggcgtatcc agacactcct ctttttgata atactgaggg cattccacct
     1201 cgtacagccg cttcatggaa gcttcgatct gctcgtaggt gtgcccgaat ttggacatct
     1261 gataggacag gtacagttcc ttttcttcct gcggatttag gtgcatggct tttgccagct
     1321 gttccaccag tgaaaaatct gtgggaagaa tcccaccgtt tttgatcttg ctgaaacggg
     1381 acttccgaat ggacagtgtt tcgcatagtt ccaccgattt caggtgtttc tgccgcatca
     1441 gacgggtcag ggtttcgcta aattgtgaca tgatgttttc attcttctcc caattcaaaa
     1501 agcattacac atgcttttga tttcgtgttt tccaatccaa tcaaacatgc acactctttt
     1561 gcatgtttag atttatatta tactatattt tcgcttgtgt cgcaatatct tttgaaatgt
     1621 ttccacttaa aaaatgctgg caggaaacat ttttagtcaa agtctggaat attcgcacaa
     1681 aatcgggaaa aactgttggt aaattagaat acgttca
//
LOCUS       DAWBGR010000311         1716 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_15505_length_1716_cov_3.507526, whole genome shotgun sequence.
ACCESSION   DAWBGR010000311 DAWBGR010000000
VERSION     DAWBGR010000311.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15505_length_1716_cov_3.507526"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          450 a          433 c          444 g          389 t
ORIGIN      
        1 agcagatggc gtgcaaagtc gccagacgct ctttgtgcgg tattgccttt acagaaatca
       61 cacataatat tgtagtaaga aaggttggaa ttttttctca aatggtaggt ttaattttat
      121 tgcagatcgc cctgatcgcc ctgaacgccg tattcgccgg cacagagatc gcggtgatct
      181 ccgtcaacga ggcaaaactg gaaaaagaag tggaaaacgg caacaagcgt gcccgctggc
      241 tgttgaaaat ggtgcggaac tcctccggat ttctggcaac gattcaggtg gcgatcaccc
      301 tgtccggctt tctgggtgcg gcatttgcct ccgacagctt cgcggaaaag ctgacggggc
      361 tgcttcaaaa gaccggcatc gggttgagta cctctgtgct gaacacgatt tccgtcattc
      421 tggtaacact ggtcatctcc tatttttcca ttgtctttgg cgaaatggtg ccgaaacgca
      481 ttgccatgaa gaatgccgaa aaattttcgc tgacaatgtc cggaccgatc acggtaattt
      541 ccaagctgtt cgccccgctg gtgtggctgc tgaccaaatc caccaacgga atcctgcggc
      601 tctgcggcat tgaccccaac agtgaggacg agatcgtcac ggaagaagac ctgctgctca
      661 tggcagatgc gggacacaaa aaaggaacga tcgacaccga agaaaacgac ctcattaaaa
      721 atgtatttgc atttgacgat ctgactgtcg gcgagatctg cacccaccgc aaggaagtgg
      781 cggtgctctg gctggacgaa accattgctc aatgggaaca gactattcat gaatcccgcc
      841 actcggtcta tccggtctgc ggggacagcg tagaccagat cgtaggcgtg ctcaacgcca
      901 aggacttttt ccggctgcaa aaccgcacca aggaattcgt catggaacac gctgtacaca
      961 cgccctattt cattcacgaa accatgaaag ctgacgagtt gttcagcaag atgaaacggc
     1021 acgcagacca ttttgcagtc gttgtggacg aatacggcgg catgtgcggt atcattactg
     1081 tgaccgatct ggtggaacag ctggtcggag atttcgacga tgacgaaacc gaacggcagg
     1141 aaccggaact ggaacgtctg gattcgaaaa cgtggaagat ccgcggcacc tgttccctcc
     1201 acgatgtggc aaaggctctg gatcacgaat tttccgatgc ggaggaatac gaaaccttca
     1261 gcggctttct catcggcaag ctgggcgaga ttccaaagga cggcagccgc ctgaagctgg
     1321 agatcgaggg atacctgatc cgcattctca acgtccggca gcactgtgtg gaaaaggcaa
     1381 tcgtccggct ggagcattct ccggcaaatc cactgtaaca gaagactttt ccggagaaaa
     1441 cagcattaga atacaaaaaa gcatctcgtt gagatgcttt ttttcatgtg cggtgttgtg
     1501 aaattcagtg tttccttagg gcaatacctc agactgccct ctccagcttc gacaaaacgc
     1561 aacttatttt ttacgcagat tttacatagc caccgtgcag actttacaga gaaccggtat
     1621 aataatggca gtacaacgca agcactttgc ggtgttatcc cagggcaata ccgcacaaag
     1681 agcgtctggc gactttgcac gccatctgct tgcaaa
//
LOCUS       DAWBGR010000312         1715 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_15516_length_1715_cov_4.024096, whole genome shotgun sequence.
ACCESSION   DAWBGR010000312 DAWBGR010000000
VERSION     DAWBGR010000312.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15516_length_1715_cov_4.024096"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          366 a          476 c          450 g          423 t
ORIGIN      
        1 ggtcagcggc gacccgccgc gggcgtaggg tcagtggcga cccgccgccg ctgttgcaaa
       61 cgggttctct gtggggtagc gtatgccctt gggctggttg tagccgatct ctcctgtgtc
      121 tacgcctaag tacttgaata cctcgtagat tgccttgccg tagtgcccga atgctaccgc
      181 accgtggtgc gggaagtggt gctctaccag tacgtgccga taaaaccgtg ccatttccgg
      241 aatcgcaaaa atgccgatgg aaccaaaaga atgtgtcgct gccggcagta cctctccctc
      301 tgctacgtaa gcattcagct tcgcatcaga ggtggactgg agccggaaga atgtcacatc
      361 gcccggcaga atgtcgccct ccagtgtgcc ctgtgtcact tcctccggca ggctccgtgc
      421 catgatcagc tggtatttca tttcgcagaa cgccagcttc ttggtggtgg tgttgccgca
      481 gtggaatccc ataaaggtgt ccgtcagacg gtacgggaac ttccctgcaa tttccttttc
      541 atacaggtct gccggtaccg tgttgttgat gtccagcaga gtgacagcgt cttcgctgac
      601 cactgtgccg atgaattcgc tgagtgctcc gtagatgtcc acttcgcagg aaaccggaat
      661 ccctctgccg gtcagacggc tgttcacata acagggcaca aatccaaact gagtctggaa
      721 tgccggccag cacttccctg caatggcaac aaattcccga taacccttgt gctcctcgat
      781 ccagtccagc agcgtcagtt cgtactgtgc cagcttcggc agaatgtccg gcttcttgtt
      841 gccgtcaccc aactctgctg ccatgtctgc cgcaacttcc ggaatccgcg gatcgttttc
      901 gtgcttgtgg aacgcttcaa acagatccag ttcggagttt tcttcgatct ccacgcccag
      961 attgtacagc tgctgaatgg gtgcattaca tgccagaaag ttcagcggac gggggccaaa
     1021 ggaaatgatc ttcaggtggt tcagcccgta gaccgcccgt gcaatgggca ggaaatcgtg
     1081 gatcatgtcc gcacactctg cggcagttcc taccggatac tccggaatgt acgctttcac
     1141 gccacgcagc ttcagattgt agctggcgtt gagcatgccg cagtacgcat caccgcggct
     1201 ggaagaaagc acgccggtgt tttcctcggc agcggcacag aacatcttcg gaccgtcaaa
     1261 gtgctttgcc agcagcgttt cggcaatttc cggaccgaag ttccccagat agacgcacag
     1321 ggcattgcag ccggcttttt tcaggtcgtc cagagcctgt accatatgga tctcgctctc
     1381 cacgatacag actggacatt cgtaaatatc ttctgcgttg tacttttggg tataggcatc
     1441 gaccagagcc ttgcggcggt tcacggacag actttccgga aagcagtcac ggctgactgc
     1501 aacgatgccg actttcattt tcggaatatt gatcataagg cagtttcctt cctgcacctg
     1561 tggtgcgctt gatttgtaac tatcatagca taacgattgt cggattacta gcaaaaacgt
     1621 acattttctg atgaaaatca tatcagaatc gtatatttcg tgggcaatac cgcacaaaga
     1681 ttgtgcgaca gatgcgtcgt caaatttttc gtcag
//
LOCUS       DAWBGR010000313         1685 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_15915_length_1685_cov_5.038037, whole genome shotgun sequence.
ACCESSION   DAWBGR010000313 DAWBGR010000000
VERSION     DAWBGR010000313.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15915_length_1685_cov_5.038037"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          357 a          546 c          441 g          341 t
ORIGIN      
        1 gcaagcccaa ggcggacagc gaggaacgag cgaattccgc cgaacggttt cgacacgact
       61 gccccagaaa gtacacttca cgaagaaccc ctacaccgtc tgaaacaaaa acgtttcatc
      121 ttccagcagc gaccggcata gccggcggta tgcctgtacc agcaagccgg caaactccag
      181 cttctgggca gcgtctgtgt tctgtgcgat cgttccggca aaggtgcagg ctgcccgatc
      241 tctggcggcg gtgatctccg tgtaccggag aaacgccacc gctgccgcca gccagttcag
      301 acgggcatcg tcctcgtctg ctccgtcctt tagagcctgc tgcacttcca gaaccgcttc
      361 gtccagaaca ggctgtaccg cgtcaggcag ttcctcctcg ccggcaaaca gcaaaaacag
      421 cgagcggatc tgattttgta ccatttttcg aatccctcct cagcaattct ccaactgttg
      481 ttccagctgt gccgcctgtg cgttccacag ccgtgcctgt gcatactcgg tttcgtcctg
      541 tagattgatg ttgctccact ccaccgaaac acctgccgca gaccccgcca gccgcaggaa
      601 tgcgatcccc acacggcgga gcaccggctc cagcaggcgg cggtaatatt ctaattcaga
      661 agtcagaata tccgcctgct ggctgctcat gcgttcggta gaagtccagt tcagccccag
      721 cagaaacggc ggaatggaca gcttggcgat cagctgttcc aacagctgcc gcaccggcac
      781 ttccgtatcc agcagggcat tgtccgcccc gatgactcga atgtccacat cgccggcaca
      841 gataaaatcc cgaatctcgc cctgtgtgcc ggcacgcatg ccctcgctcc actctctggc
      901 gatctgtctt gcccgttcgc cggcataggc acgttcagcc ggatcggcag agggcttata
      961 ggtaacagca tagcggacgt tgcccatgcg gtcgtaattc tgcccgatac actcaaagat
     1021 ccgcagcaga atccggctca atgccggcag tccacgcaat accgatactc cataaacacc
     1081 gccggcaggg ggatttagtg ccgtaaacag gatgcgttcc ggtcttgcga tccgcaccat
     1141 gtccgattcc tgctccccgc gaagccagta ctgccgttcc agcggctgac tgccggagcg
     1201 aatctgtacc aactctgcgg ggactgtctg taagccgccc agaaagccgg tgcggttgtc
     1261 gatcagcatt tcccccagag cattgccgta ggtcaacagg ctgtccagat aacagtccgc
     1321 aaaactttgc agggactgtc cggtcagccc caccggcacg ttctgtacaa aatcgtccag
     1381 ctgcggctgc atgcgtctgt cctcgcacac cagccggaaa ctgccggtga gccggacgat
     1441 ttttccgatc gcggcatcga tcaccggcac cgcccgacgc agtccggtgt acagtgccgc
     1501 ttcacaggga ttcggcgttt ccggcacggc ataaggctgt tcctgccgtg ccgcagtctg
     1561 taaaatgccg ccgcccacag gcggtttctt tttcctcgga aataatccca tacattctga
     1621 cgggaaaagg agggcaatac cgcacaaaga ttgtgcgaca gatgcgtcgt caaatttttc
     1681 gtcag
//
LOCUS       DAWBGR010000314         1671 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_16117_length_1671_cov_4.102104, whole genome shotgun sequence.
ACCESSION   DAWBGR010000314 DAWBGR010000000
VERSION     DAWBGR010000314.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16117_length_1671_cov_4.102104"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          350 a          417 c          487 g          417 t
ORIGIN      
        1 tgccgaaaaa tctgacgacg catcttacgc acaagcttta ttcagtgttt ccttaagcat
       61 gtgattttgt gaagtgctgt ttttatgcac agacatagta ctggttgtcc agttcgatga
      121 ggaacacggt gacttcgtcg cagtagaccg tatcagtgcc gttggtgagt accggctgta
      181 cggtcagcgt tttgcagctt tccatgtggt cggtacaata gttgctgtcc tcgttcagtt
      241 cgttcagcat gccggtgagg tattccgcat tggagccggt ttcgtcctgt ttgaggcagt
      301 cgttcaccag aatctgcgag aagtgaatgt caccgctaag ctgcttttca tagctttcct
      361 tctgctgggt gacataggcg ttgtcgtcca gcagtccgcc gtaggcgttt tcatagaggg
      421 attccatgta cttttccacg gtgcaggact ggaacagttc cacgtcctcg ttctggatcg
      481 aagcgtagta gtttgccagt gcgtccagtt cgccgtcctc cagaaaacgc ttgtcgtatt
      541 ccagcggcac tgcacggctg tcgtcgtccc gcatggtggc accgtattcc agatcttcca
      601 tggcgatgtt cacgccggat tcgctgtcag aggaattgct gtcggagcag ccggcaaaac
      661 cggtgcagag cagtgcggca gcggtcgcac agaccagaat ccgccggaac gtattgcgat
      721 tgtatgtcat gaggtatcag ttcctttcga tgatatgcta ccttatactg tagcacattt
      781 tcggcaggtt gtcaaccaat atagaaaggg aaatcgttat gccgagattt tttttagaag
      841 aactagacga atctgatgtg tgcatcaccg gccctgatgc ccgccatatc ggactggctc
      901 tgcgaatgcg tgtgggggag atgctgacag tctgtgccta cgggcgggac taccagtgcc
      961 ggattcgttc cattacgccg gaagaagttc atctggacgt ggtttcctcg gaactgtgtg
     1021 cggcggaacc gtcggtgtcc ctgaccttgt atcaggcagt gcccaaagcg gacaagctgg
     1081 agcagatcat tcagaaatcc gtggaactgg gagccacacg aatcgttccc gtgctgacac
     1141 gccgctgcat ctcccgtccc aagccggcgg agtttcagaa gaagctgccg cggctgcaaa
     1201 aaatcgcggc ttctgcggca aagcaggcgg ggcgtggcat cattccggaa atcgctccca
     1261 tgctgacctt tgcacaggcc tgtgcggaga tgcagcaggc agaccgtgcg attttgctgt
     1321 acgagggcgg cggtgttcgg tttgatgagg tggacctgca cgactgccgt tccattgccc
     1381 tgatcgtggg cagcgagggc ggcttcgatc cggaagaggc ggagcagctg caacagaacg
     1441 gagcagtcgc agtctggctg gggcaccgga tcctccgctg tgaaacggca ccgctggcgg
     1501 cgatctctat tgtgatgcac cggacgggaa atctttgaaa atctaggaga gaatctagga
     1561 aaacgcaaaa aacggcatga ctttttcggt catgccgttt cgtattttct gtgaagttgc
     1621 tttttaggca ataccgcaca aagattgtgc gacagatgcg tcgtcagatt t
//
LOCUS       DAWBGR010000315         1645 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_16448_length_1645_cov_4.235849, whole genome shotgun sequence.
ACCESSION   DAWBGR010000315 DAWBGR010000000
VERSION     DAWBGR010000315.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16448_length_1645_cov_4.235849"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    719..728
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          412 a          395 c          454 g          374 t
ORIGIN      
        1 ttgcgaggac tagcacctga agctagcgcg tctgccaatt ccgccatatc cgcatagata
       61 cgctttctcg actatatgaa cttttgcatc aatgaaaaca tggtgcggat aagaggactt
      121 gaacctccac ccccttgcga ggactagcac ctgaagctag cgcgtctgcc aattccgcca
      181 tatccgcatt tcgcatgccg tgttttcagc gacaagaatt attatagcac aacactttcg
      241 atttgtcaag cctttttttc aaattttttc gattttttct gatttctttg agagtgccga
      301 aaagtcacgc aatttcaggg cttttcttct acctgcacaa atacttgcac aaaatgataa
      361 actgtgatat aatagaaaag cattattccg aatcgaattc tggaatcatt tgtaggggga
      421 atcacaatgg aacattattt ggataacgct gcaacgacca agccgtgtgc agaagctgct
      481 gcggcagtga tgagctgcat ctctgcacat tacggcaatc cgtcctcgct gcaccgcaag
      541 ggactggagg cacagcacga agtggaatac gcccgcaatc agatcgccgg tgctctgggc
      601 tgcaaggcac aggaaatcgt tttcacttcc ggtgcgacag aaagttccaa tctggcgatc
      661 cgcggggcgg cggcagcata cggcagaaag cgaaagaaaa tcgttgccag tgctgtggnn
      721 nnnnnnnngc tgtggagcac gcttctgtga aagcaactct ttcctatctg aaaacgctgg
      781 gctttacggt cgttaccgtg ccgccggctg cggacggaga gtatcataca gaagactttc
      841 tgcgggaaat agacagcagc gtctgtctgg tgagcatgat gctggtgaac aatgaaaacg
      901 gctgccggct gccggtggaa aacgtattcc gcatggtgaa gcgaaatgat ccggaaatca
      961 tcacacactg tgatgccgta cagggattcc tgaaaattcc gtttaagccg agaacactct
     1021 gtgccgatct ggtttcagtc agcgggcaca aggtacatgc tttaaaaggt gtcggtgctc
     1081 tgtatatccg gcatggcgtg cgggtgaaac cgatcctgtg cggcagcgaa caggaacggg
     1141 ggctgcgtcc gggcacagaa agtgtgccgt tgattgcttc gttcggggcg gcaacggaaa
     1201 agctgtccct gaacatcgaa gaacgtctgg aacgggtttc tcagcttcgc agcagtctga
     1261 tcgcacagct gcgggaaatg gacggcgtga tcattcggga catagaggga gcatctccct
     1321 atgtgctgaa catttccctg ctgggctatc gctcggagat cgtgctgcac tatctggagc
     1381 agcgggaagt ctatgtttcc agcggctcgg catgttccaa gggagccagc agcggcgtac
     1441 tggaattatt cggggcaaag cccgcagagg tggacagtgc cctgcgaatc agcttctgtg
     1501 cagagaacac agaggaggac attgcggcac tggtacaggg actgcgggac gcacagaaag
     1561 aactgatgca ctgatacggc agaaatgctg aaggcaatac cgcacaaaga ttgtgcgaca
     1621 gatgcgtcgt caaatttttc gtcag
//
LOCUS       DAWBGR010000316         1617 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_16839_length_1617_cov_4.963508, whole genome shotgun sequence.
ACCESSION   DAWBGR010000316 DAWBGR010000000
VERSION     DAWBGR010000316.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16839_length_1617_cov_4.963508"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          351 a          467 c          512 g          287 t
ORIGIN      
        1 aatcccccgg cggggcgaac aggcggctgc tgccgccgta acattaccct ttttacactt
       61 gaaattgggg cgtagggtca gcggcgaccc gccgcggggc gtaaggtcag cggtgacccg
      121 ccgcagaagg agaatctatt atgcatacca ttgcagcaat cgcaacccca cacgctgccg
      181 gcggcattgc cgtgatccgt atttccggtg ataccgcaat ctccgttgca cagcagtgct
      241 tccggagcac cggcggaaaa tctctcgccg agatgtccgg ctatacctgt gtctatggca
      301 gctttctgga taccgccggc agcgaactgg acgacggcat cgccactgtg ttccgtgccc
      361 cgcatagcta taccggcgag gacaccgtgg aattttcctg tcacggcggc atttttgtca
      421 cccgccagtt gctggaaaca gtctacgctg ccggtgtcca gccggcagaa gccggagaat
      481 tcaccaagcg ggcattcctt gccggcaaaa tgacactgac acaggcggaa gccgtcatgg
      541 acgtgatcgg tgccgccgga aaacgggaac tggcattcgc ccacgctcag cagaacggag
      601 cactgtttca ccgcgtgcag cagatctcgc agggcatcgt ccgcagtctg ggagatctgg
      661 cagcgtgggc agactacccc gaggacgaga tccccgcagt gacaccggag agcctgcacg
      721 ctgccctcca gccggtgctg gaacagctgg acagtaccct tgctacctac gactatggca
      781 gaatcttgca gtccggcgtg tcggcggtca ttgtgggcaa gcccaacgtg ggcaaatcca
      841 ctctgttcaa tctcctgtcc ggcagccagc ggagcattgt cacggaaatt gccggcacta
      901 cgcgggacgt ggtggaagaa cagatccggc tgggcaatgt gacactgcgg ctgtcagata
      961 cggcaggact gcgggaaacc gaagatgtga tcgagcaaat gggcgtgaag attgccgagg
     1021 aacggctggc acaggcagac ctgattctcg ccgtgttcga caccacccga ccgctggacg
     1081 acgacgaccg ccgcatgctg gaacagctgc cggagaagcc ggtgatcgca atcctgaata
     1141 agtctgacca gtctgcacag ctggacacgg acgaactgga gccgcatttc tcccgcgtaa
     1201 ttaccatggc agccagagag ggcagcgggc tggagcaatt gcagcaggcg gtggaggaac
     1261 tgttctatca ggcagaagtc acaccggaac tgggcattgt ggcaaacgcc cgccagaagc
     1321 agtgtctgga gcaggcacgg gagcaggtgc agctggcact ggaagcactg gacgcagggg
     1381 aactgctgga cgcggtgact gtcctgctgg aagaagccgc gaacgccttg ctcaccctca
     1441 ccggcgaacg ggtttccgag gccgtggtaa acgatgtatt ctcccgcttc tgcgtgggga
     1501 aataatggga tggtgacttt ttgaaatgtg tagggcgatg cccacatcac cccgcggact
     1561 tgtgcataag cctcccctga aaggggaggg ggaccagcga agctggtgga ggggttc
//
LOCUS       DAWBGR010000317         1598 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_17118_length_1598_cov_4.514582, whole genome shotgun sequence.
ACCESSION   DAWBGR010000317 DAWBGR010000000
VERSION     DAWBGR010000317.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17118_length_1598_cov_4.514582"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          380 a          424 c          370 g          424 t
ORIGIN      
        1 tgcaagcaga tggcgtgcaa agtcgccaga cgctctttgt gcggtattgc cttaagtaca
       61 agtataccat aacacgcccc gtttgtcact acttttccgc acattttgga gatttgtgca
      121 aaatcgaaag ggaaagctgt taattttttg acaaatgaaa gaaaagtggg cttgctgtgg
      181 ggagaattgc cgttttccgc aagggcagac ggagaaattt tcccatatcc attattgcct
      241 catcaatgat gccactatga tttttcaaca ttttcgcata ttctcattga caattgccat
      301 agaatatggt atactaaaca tacactacag aaactgacga tctgcggcag cccgcaaacc
      361 gtccgatttc aattcatatt aaaggagaat acttatgatt tgtccgaaat gcggcagcaa
      421 caattctgac ggcacagcat tctgtgcaag ctgcggagct ccgctgtcca atgaagcacc
      481 gactcccaac ggagcagttc cgaacaacaa cgttccgccc cagacaccgc cgccggtatt
      541 caacggcaac ccctacggcg gtcccatcga gcagcgtaac attgcactgt gtatcatttt
      601 gtcgatcgtg acatgcggca tttacatgtg gtactggatc tacaagctga ccgaagatgt
      661 caacaaactg acaggtgatc ccaatgccac ttccggcggc atggtcattc tgctgagcat
      721 catcacctgc aacgtctaca tgtggtactg gctctacaag cagggcgatg ccattgatca
      781 ggtgaaagcg tcccgcggac tgccctccgg caacagcggc attctctatc tgattctggc
      841 aattttcggg ctgggtatcg tatcctatgc cctgatgcag aacgaactga accagctggc
      901 ataagagcct gtgcttttcc cgctgaacaa aacacaaaag ctgcacggaa cgattccggt
      961 gcagcttttt tctgtacgac aaactttccc gcaaccggca aggaggtatc ccttttgctg
     1021 caaaatctgc gtttcttttt ccgcatccac cgcaaacagc tccgaactgc cggcatcttt
     1081 tttctgctgg ggctggcgta ttatatcgtc acccagctga caccgctgcg gatcccctgc
     1141 ctgttccaga aggtcaccgg tctggcatgt ccgggctgcg gcatctctca tttttgcatt
     1201 cgcctgctgc atctggattt ctttggtgcc gccagggaaa atctggcgat cgctctgctc
     1261 tctcccctgt ggctggcggc atttctggtt cgggtgctgt ggaatccaaa gtggttcgcc
     1321 aagggcagca aaggggaaaa tcttctgctg tggggcagcg ttgttgtgct tctgctgttc
     1381 ggcgttgtcc gcaatcttcc ccatatggaa tttctcctgc cgtcctatct gcaatagcac
     1441 tcctttttcc ctccgcgaat ataataaaac agtatcgatt atttttgaaa atcacgggca
     1501 tactaagagc gtgatcacac aaagccattc atcgttgtgt tttctaaggc aataccgcac
     1561 aaagattgtg cgacagatgc gtcgtcaaat ttttcgtc
//
LOCUS       DAWBGR010000318         1559 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_17738_length_1559_cov_4.626330, whole genome shotgun sequence.
ACCESSION   DAWBGR010000318 DAWBGR010000000
VERSION     DAWBGR010000318.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17738_length_1559_cov_4.626330"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          324 a          423 c          377 g          435 t
ORIGIN      
        1 ctgccgaaaa atctgacgac gcatcttacg cacaagcttt atgcagtgtt tcctttattc
       61 agcgttttcc aatttttcgt attctgcatc gcttaccggc tcgcaccatt cgttgctgca
      121 gttttcgccg gggcattcca ctgcaatgtg gctgaaccag ctgtcctttt tggcaccgtg
      181 ccagtgtttg acctctgccg gaatggtgat tactgtgccg gctttcaggc tgacggcagg
      241 cttgccctcc tcctgatacc agccctctcc ggcagtgcag atcaggatct gtccgccgcc
      301 cttggtggca tggtgaatgt gccagttgtt ccggcatctc ggttcaaagg ttacgtttgc
      361 cagaaaaacc ggtgccttct ccggttcagt caggggtttc agaaacgact gcccgataaa
      421 atactgggcg tatgtgtcgt tgggagcacc gatgccgaat gcatcagctg cttcaaatgc
      481 ggtttttttg gtgtatttca tggaagattt ctccttagtc atgcaccttc agctggtgca
      541 acatttttac aaagtccgga ttgttatggt ctacgatttc gctgtgtcca atgtccagct
      601 ttgcaatctc tgccatatcc tctgccgaca aggtgaaatt ccagatgtcg aagttctgtg
      661 ccatacgttc cgccttgacc gacttcggaa tgacaacaac gccacgctgg acattccaac
      721 gcagtgccac ctgtgcggca cttttgccgt atttggctcc gatcgcagtg agtaccggat
      781 gcgtgaagat gtcgtgattg ccctcggcaa aaggacccca tgcctccggt gtcacaccgt
      841 atttctgcat cagctttaaa gcatctgcct gctggaagaa tgggtgcagt tccacctgat
      901 ttactgccgg aatcacggaa accgtttcac acaggtctgc cagccgtgcc ggatagaagt
      961 tgctgacacc gatcgcccgc agctttccgg cgtggtatgc ttcttccatg gcacgataag
     1021 cacctacata atcacccatt ggctggtgga tcagatataa gtccagatag ttcagcccca
     1081 gcttttgtaa ggaagtgtca atggcttttt gggcagcttc atagcttgcg tcctgtaccc
     1141 agagtttggt ggtgatgaac aatttttcgc gaggaacttt gcttttggcg atcgctgctc
     1201 ccacagcttc ttcattataa taggcagcgg cagtgtcgat cagccgatag cctgcggcaa
     1261 tggcttctgt cactgcctgt tcgcaaattg cggcatcggg tacctgaaat acgccgaacc
     1321 cttccagcgg catttttacg ccgttattca atgtaataaa ttccatttgt catacctcct
     1381 gattttgggc tgtagcgatc cgtagaagca ggaaaccgtt gtaccgccag ccaatccgtt
     1441 atttagagca gcaccgcaca aagcccgcct gacggctttg cacacaatct gcttgcagat
     1501 tttccgtcat attgcccaga aatcccttgc catacggcag tatgcctgcg gaatttcta
//
LOCUS       DAWBGR010000319         1529 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_18195_length_1529_cov_5.010855, whole genome shotgun sequence.
ACCESSION   DAWBGR010000319 DAWBGR010000000
VERSION     DAWBGR010000319.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18195_length_1529_cov_5.010855"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          404 a          403 c          408 g          314 t
ORIGIN      
        1 aaagggaggg ggaccgccac taggcggtgg agggattctc cgccacagca gccaacaggt
       61 ttcataagaa gcaaaaaaag aaaagagttc gcatttcacc tgtactgtaa gattccaata
      121 tcacacgagg attacacgag aaactgtcat catagattct caccaacaaa actcccccgg
      181 cggggcaaac aggcggctgc cgccgccgta acactaccct tttcacaatt gaaattgggg
      241 cgtaaggtca gcggcgactc gccgcacgcc gcagaataga aataatgcaa gttcctatag
      301 aatttgtact tgattttttc tgcaaaatcc gctatgataa agttagcaac tcaatacgct
      361 ctcacagaac ggaggaatct gatcatgaca gaagaagaaa aaatgcttgc cggaaaactc
      421 tatgacccca gcgaccccaa actggatgcc cgccggctgc tggctcaccg gctttccatg
      481 gaatacaaca acacactgga aaccgatgcc gaaaaacgca aagccattct ggcacagctg
      541 ctgcccgatt gcggagagga cacatttctg caagggccgc tgcaatttga ctacggctgc
      601 tatacccgct tcggcaaacg ctgctacgcc aacttcaacc tggtcgttct ggacgtgtgt
      661 ccggtgactt tcggcgatga cgtgttcatt ggcccgaact gctcgttcgt ttctcctatg
      721 cacgccctgc taccggaaga acgcaacctg aaacagcgtc cggacggctc ctactatgat
      781 ctggaatacg gcaagcccat taccgtgggc agcaactgct ggttcggcag caacgtcacc
      841 gtctgcggcg gcgtgaccat tggtgagggc tgcgtgatcg gtgcaggcag cgtggtgacc
      901 aaagacattc cgccccattc gctggcggtg ggcaacccct gccgtgtgct gcgggaaatt
      961 acggaaaagg acagcgtgca gtataaagga atcactgcat aaagcccgcc tgcatgccaa
     1021 tattataatt ggaaaatctc ccgcacttca cacagaaagg acgattcata tggcagtgat
     1081 tcgattgaca aaggaaaatt ttcaggcaga agtgatgcag gcggacaagc cggtgctgat
     1141 cgacttctat gcagactggt gcggtccgtg ccagatggtt tcgccgatcg tggacgagat
     1201 cgccggcgaa cgcagtgaca tcaaggtctg caaggtgaat gtggacgaac aaatggaact
     1261 ggcacagtct ttcggtgtgt ccagtattcc cacgctggtg gtgatctccg gcggaaagct
     1321 ggtaaagcat gccgtgggtg cacagccgaa gtctgcgatt ctggacatgc tggaagctgt
     1381 agaatgatcc acatattccc gaaaaaaaca aagccgctgt cctgtcagtt tcacggggca
     1441 gcggcttttt tgcggagatg cggcaaaagt ccacgggcaa taccgcacaa agagcgtctg
     1501 gcgactttgc acgccatctg cttgcaaat
//
LOCUS       DAWBGR010000320         1527 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_18225_length_1527_cov_4.859375, whole genome shotgun sequence.
ACCESSION   DAWBGR010000320 DAWBGR010000000
VERSION     DAWBGR010000320.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18225_length_1527_cov_4.859375"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          321 a          415 c          454 g          337 t
ORIGIN      
        1 ggagcagacc gtctactggt atgctctttc tatctttatt atacgccaga acgccgtatt
       61 tgtcaagagc ttttttgccg ggaaatgcgg ttatttttat gccatgagat tccgcagaaa
      121 caaaactacg tactttttgc gtacttttgt acttttctac aatgcagttt gcaccatgcc
      181 gcaatgcccc tacagaccgc caaaaaccgc acctccgctg ttcctgccgc atacgataaa
      241 ccagcagaag gaggaatgca tatgcctttg atcagtcttt gcatgatcgt gcgaaatgaa
      301 gaagcggtgc tggggcggtg tctggattcg gtggcagatc tggtagatga gatcattttg
      361 gtggacaccg gctccaccga caacaccaag gcagtggctg cggaatatgc cgccaaaatt
      421 tatgattttc cgtggtgcga cgatttttcc gcggcacgga actacgccgt ttcccaagcc
      481 gtaggagatt actggctgtg gctggacgcg gacgatgtca tcgaggggga aaaccacgaa
      541 aagctgcgaa aaatcctgga ggctccggaa gcagacatgg tatttcttcc ttactggctc
      601 agctttgacg ctgccggaac gccccagatg atctcccggc gagaacggat tttccggcgg
      661 agccgcggct atcgtttcac cggagccgta catgaagccg tggtgccctc cggcagtatc
      721 cgctacggcg atgcggcagt gtcccaccgg aagccgcacg ccggcgaccc tgaccggaat
      781 ctgcacattt atcagaaact actgctgtcc ggcaaacgat tttcgccgcg ggagcagtac
      841 tattatgcaa gggagctttg cgaccacgga gcctatcggg cggcactgcc ggtgctggaa
      901 cagtttttgc aggagggcag gggctggaca ccggacaaca tcggtgcatg cctgcttgca
      961 ggtcgctgct atgcccggct ggaacagccc gacgaggcaa tgcaaagtct gttccgttcg
     1021 ttctgctacg gcattcccca gccggagatc tgctgcgaga tcggcgggct gtttctggag
     1081 cggcagcagt acccgcaggc agctttctgg tatcatctgg caatcgaaac cggtcaccag
     1141 ccccacgagg gcttctggcg gcaggagtgc tgtgactatc tgccggcgat ccagctgtgc
     1201 gtgtgctatg accgtatggg tgacatttcc acggcggcag catataatgc attggcaggc
     1261 agttttcgcc gcggcgatgc ggcagtggcg tataaccggc agtatttcgc ggaaaaggct
     1321 gcccgccgga aatgatgcga aaggagcgat atagaatggc atattgtaat tgcggagaac
     1381 ccagccagcc tgaccgctgc tgcatgccgg caccgccgcc tgtgccctgt tgtctgcaag
     1441 gtccggcagg tgtgaccggt ccggtcggtg cgacaggtcc taccggtgcc acaggtgcgg
     1501 acggtgccgt tggtgcaaca ggtccta
//
LOCUS       DAWBGR010000321         1521 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            NODE_18300_length_1521_cov_4.313779, whole genome shotgun sequence.
ACCESSION   DAWBGR010000321 DAWBGR010000000
VERSION     DAWBGR010000321.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18300_length_1521_cov_4.313779"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          444 a          340 c          353 g          384 t
ORIGIN      
        1 aaaccgtgcg tttcaggatt gcccaaattt gagtaaggta actatttcgg acagtgtaat
       61 tgattttgga gaaaatgtat ttaccagaga tacaagtttg cggactgtga aacttccggt
      121 caatctaaaa gaaatgggaa caggaacgtt ttctggttgc accagtttga caaaaatgac
      181 attgccgaaa acgcttccaa cagtgccgca agatacattt tctggctgca aaaatctgaa
      241 tacggtttca gttccggagg gcattgtgtc attgggacgt aatgctttta tggaatgctc
      301 caatttggaa tctattacgc ttcccagtag tttagggagt atggagagca gtgcatttga
      361 aggttgtact gcactgaatt caattgcgat tccggacgct gtattggaat tgccgagcag
      421 agcttttgat ggctgtacca gcttgaaaga agtgacattg cctgaggaat tgactgcaat
      481 tgggggacag gcttttgctg gttgttccag tttgaaagaa attacacttc cggctggcgt
      541 gaaaacaatt ggggatagtg cattttttga gtgcggaaac ttggaaaatg ttacattctc
      601 agaaaaatta actcaaattg gaagcaaagc atttgaaaac tgcacaaagt tgaataacgt
      661 aactttccct gatagtctgg tgtctattgg atttgcagca ttttcaaatt gcaaggcatt
      721 tacaaaaatt gtaattccgg aaaatgtggc agagttacat tatgattctt ttacaggttt
      781 gagttatacg tttagcggtt gcagcaatgt ttatgcggtt caaattccta agagcgttat
      841 tgcaattgca ggcagtgctt ttagcggtga tatttccgat gtttactaca caggttcgga
      901 agaagattgg ggtaaaatga gcattcatag caataataaa tctttgctga acgcaaccat
      961 ccactacaac tggaacccca acgatcccat tcccgaccag accgtaacta ccactgaaac
     1021 cacaaccgaa accaccacta ccaccacaac aacgacaact accacgatca cgacaaaatc
     1081 caccactgaa cagacgacca acaccacgac caataccaca aacaccacca cggcgacaac
     1141 cgcaacgact gcaaccacta ccacaacggc tccggcagct gcaaagggtg acgcttccgg
     1201 tgacggcgtg ctcgacacca acgacgtatt cgaagccatg ctctacgtgg catactgcgg
     1261 tgcaggcatg tccggcaacc tcaccgacga ccagatcgcc gctgccgata ttgacggtga
     1321 cggctcggta gattccaccg acgtgtacta tatcctctac tacgttgccc tgcagggtgc
     1381 aggcaagaat ccgacttggg acttcatact gggcagaaag taattcctag ggaaacacgg
     1441 cataaagctt gtgcgtaaga tgcgtcgtca gatttttcgg cagattgcat agaaattccg
     1501 caggcagact tggtgtctgg c
//
LOCUS       DAWBGR010000322         1391 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            k99_2680_length_1391_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWBGR010000322 DAWBGR010000000
VERSION     DAWBGR010000322.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_2680_length_1391_cov_6.0000"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          407 a          302 c          360 g          322 t
ORIGIN      
        1 gaattatgga gcaggaattt actttccttc atgaataaac aatcatccat gtccagcaac
       61 cgatttcaaa aaaagtttta tgcaaaggat tttacaggaa aaattgtgat atttcaatgg
      121 cggaattaga gttactcttg tttgttcata aaatattgta gatattttgc cagaaaaacg
      181 ctataatata tagcagattg cacgaatctt atcaggacat ttctgaaaga agtatcaaaa
      241 ataggaggaa tcacttatga aacgcagaat cggaatttta accagcggcg gcgactgccc
      301 gggactgaac gccacaattc gcggcgttgc aaaggcttgt tatgaaatgc tggggacgga
      361 aaatgtagag atcgttggaa tttcagacgg ctattatggc ttgatccaca acatctgcaa
      421 ggagatgtca ccggacgatt tctccggcat tctcacacgc ggcggtacga ttctgggtac
      481 caaacgtcag ccgttcaaga tgatgaaagt catcggcgat gacaatgtgg ataaggtcaa
      541 gaacatgaaa aagacctatc aggatcagaa gctggattgc cttttgacac tgggcggaaa
      601 cggcacacat aagacctcca agctgctggc ggacgaagga ctgaatgtca ttggtctgcc
      661 gaaaaccatt gataatgaca tcttcggtac agatgtgact ttcggattcc atactgcggt
      721 agacattgcc acagatgcac tggatcgact gcacaccact gcaaacagcc attcccgtat
      781 cctgctctgc gagatcatgg gcaataaggc tggctggctg actttgagtg caggcattgc
      841 cggcggtgcg gacatcattt tgattccgga aattccgtat gacattgaca aggtttgcaa
      901 gtccgtgcag aagcgtagcg aaagcggcaa aaacttctct atcgtcgctg tggcagaggg
      961 ctgtttcgac aaagaagaag ccaagatgaa gaagaaagaa cgcaccagac tgcgtcagga
     1021 agctggcatc accactgcga ccaaccggat cgcagcacag attcagcaaa agaccggact
     1081 ggaagcccgc gtttgcgtac cggggcacat gctccgtggc ggaagcccaa gtgcgtatga
     1141 ccgcgttctg gcaacacagt tcggcgtata cgcagcagaa ctgattcggg aggaacatta
     1201 cggtatgtct gtagcaaagg ttggcggcac gatcactgcc aataagctgg acgacattgc
     1261 cggtaagaca aagtttgttt cgccggacga tcagctggta cagactgcaa gaaatctggg
     1321 aatttctttc ggcgactgag tagagttatt gaaatctatc caagaagcgg gtgcattttc
     1381 gatgtgtccg c
//
LOCUS       DAWBGR010000323         1297 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            k99_11141_length_1297_cov_7.0000, whole genome shotgun sequence.
ACCESSION   DAWBGR010000323 DAWBGR010000000
VERSION     DAWBGR010000323.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_11141_length_1297_cov_7.0000"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          370 a          287 c          347 g          293 t
ORIGIN      
        1 aattatattg taacaggagt gatcgtaatg ttttgtcaga actgcgggaa aaagattgag
       61 ggaggggact tgttctgtcc gttttgcggg gcaaaacagc agcaggtgaa cccgtcagaa
      121 aatggtggtg cttcgttcac tgccggctca caaaagctca ccagaaaagg gttgggcatt
      181 gccgtgatct gtgtgctgat cgtcattgtg ctggcgttgg tgctgatcgg ttccctgtgc
      241 accggcggac cgaaacaggt agtcaaggag tactatcagg caattgaaaa gtgtgatgcc
      301 aagaagctgc tggacaccgt cccgaaagac tacctgaaag aagtcatgga cgaaaaagac
      361 ctgaccaaac gggaactgct caaggaagtg caggcgtatc tggacaagta ctacagcggc
      421 tatgacgaca tcaaagtcag cattaccgat caggaaaaat tggatcggga cgatttttcc
      481 gactattttg actttgatct ggaagaagat gcagatctgg agatcaaaaa ggcagtgcag
      541 tttgacctga aggttcgcta tcagtctgac gaaggcaaac agacagaaaa agaggaattt
      601 atcgtatttc gatacgaaaa cggctggtac agcatggacg cggtatttct ggttgccatg
      661 tcggcctacc tttaaaacag gagaaacctc atgacaatac tacaaaccga acgcctgctg
      721 ctgcgtccgt tggctccgac agattttaca gcattccgca catttgctgc ggacggcgaa
      781 ctgacgaagt atatgctgtt ctacccgttg gagcaggaag cagaactgca cacgtttttg
      841 cagaatgctg cccaagcgtg gaacagtctt ttcccacaga cgtatgaatt tgcagtgctg
      901 cgaaaagcgg aactgctcgg cacggtcaca ttggagtaca tgacaccgga acagggcatg
      961 ctgggctggc tgtttcgtgc agatgcacac ggaaagggct atgcgacaga agccgcgaaa
     1021 gctgtggtgg agtggtgtcg ggaaaagttg ccctatcgca cattggttgc ttgctgcgat
     1081 gcacggaacg ccgcctcgga acgggtgatg caacgcattg gcatgcactt ttcggaaact
     1141 ggcagccgca catatcccca gaccggagaa acggcagaag aacgaacata catcatgaca
     1201 ctgtagaaat atcccaattc tgttctgtat tgctcatcta aaatgaaaat caaaattcac
     1261 caatatatcc atatattttt aggacaatac atctcaa
//
LOCUS       DAWBGR010000324         1178 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            k99_63888_length_1178_cov_6.0000, whole genome shotgun sequence.
ACCESSION   DAWBGR010000324 DAWBGR010000000
VERSION     DAWBGR010000324.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_63888_length_1178_cov_6.0000"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          276 a          254 c          341 g          307 t
ORIGIN      
        1 atgctactgg agcgtttttt gaagcggtgc atttctaatt atgaacaggt agaagacccg
       61 aatgtgagaa aggcatacgg gacatgcagc agtgtggtgg gaatttgctg caatattctg
      121 ttgtttgtcc tgaaatatgt catgggtacg ctttcccact cgatcgccat tgtttctgat
      181 gcgttcaata acctgtcaga ctgtgccagc tgtttgatta ccctgtttgg ctataaactg
      241 gcggcaaagc cggcagacaa gggacacccc tttgggcacg gcagaatgga gtacctgact
      301 tcactggtca ttgctgtgat gatcgccctt atggggtttg aactgctgcg gaattctgtg
      361 gaaaagatca ttcgtccgga cgcggtgcag ttccgctggc tgacgctggc ggtgctggtt
      421 ctgtcaattg gagtcaagct ttggatggcg tggttcaaca ccaaactggg gaaacggata
      481 cagtccacgg tgatgatcgc cacggcgaag gacagccgca gcgatgtggc agctaccgga
      541 gcgaccattc tggcactgct gctgtcgttg gtgacaacgc tgccggtgga cggtattatg
      601 ggtacggcag tttctctgtt cattctgaaa acggctttcg acatcattcg ggatacggtc
      661 ggcgatcttc tgggaaagcc ggcagatgtg gaaacggtgc agaagctgaa agaactggtg
      721 ctggcacatg aaaaaatcat tggtgtgcat gatctgatcc tgcacaatta cggtccgggt
      781 cggactatgg gaagctgtca tgtggaagtg cggagcgact ctgattttgt tgcggtgcat
      841 gaagttgtgg acttgatcga acgggaaatc gaggaaaagc tgcacattct gatgacgatc
      901 cacatggatc ctgtggaggt ggacaacgcc gctgtcaacc actatcggca catggcagaa
      961 caggtgattg ccgaactgca tcagaatttg agcctgcacg attttcggct ggtgtccggc
     1021 gaaaacacca atcgtttgat ttttgacata ctggtacctt ttgattgtac ctataccaat
     1081 gatgaattgt tggatatgat ttccggcgaa ctgtcccgca tggacaaaac ctgtcaggtc
     1141 agtctgacgt ttgatcggga ttttacataa ggcaatac
//
LOCUS       DAWBGR010000325         1155 bp    DNA     linear   ENV 27-AUG-2025
DEFINITION  MAG TPA_asm: Ruminococcus callidus isolate gSpreadC_ID_1374
            k99_73528_length_1155_cov_8.0000, whole genome shotgun sequence.
ACCESSION   DAWBGR010000325 DAWBGR010000000
VERSION     DAWBGR010000325.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37994462
            Sequence Read Archive: SRR3917648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus callidus (human gut metagenome)
  ORGANISM  Ruminococcus callidus
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Ruminococcus callidus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k99_73528_length_1155_cov_8.0000"
                     /isolate="gSpreadC_ID_1374"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:40519"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.829 N 96.7 W"
                     /collection_date="2016-07-18"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          294 a          284 c          285 g          292 t
ORIGIN      
        1 gctttattca gtgtttccct agattcatct taccagaaaa agtggattct gtcaactgtt
       61 ctttccggaa tattaaggga acactgcaac aagaatagca tgttccggaa tcctgcaatt
      121 atgcccttga ccttttttcg gaaaaacgct ataatgttac ttatatcaac atgacaggag
      181 gaaatataca tgaatccaat tcgaatatgc tctgccttct ggacggcttc ttctgcggtt
      241 gccacagata ccacagatct ggcacctgcc gtacagaaca tggtggacac tgtaggggaa
      301 gaagtaaagc agacttccgg catactgaac aaaatttttc agcctattct ggacaagctg
      361 ccgtccctga tctttgccat actgtttttg ctgatcggct tttttctggt aaaacagatc
      421 atgcggatcc tgaagcgggc attcaaccgt tccaacatgg acggcatcat gtccagtttc
      481 attcgttccg ttgccaagat cgtactgtat gtgctgctga tcgtcattgc tctgtccatt
      541 ctggacgtgc ccatggattc cattgttgcc gtgatcgcgt ctgccggcgt agcagtcggt
      601 cttgccctga aagacagtct ttccaatctg gcaggcggtt ttatcatttt gttctccaag
      661 ccgctgaaag agggggacac cattgaagct gacggcgtga cgggtaaggt agaagccatt
      721 accattctct acacccgcat tgtcactgcg gacaacaaga ccgtttttat cccaaacggc
      781 gtagtcgcca gcggcaagat catcaactat acagacaagg atacccgccg cgtagacctg
      841 aacttcggca tcagctatga ggacgatatt gatgctgccc gcaaggtact gctggagcag
      901 gtgcaggcgg ctccggaggc tttggcagaa ccggaagcga aagtggtggt gttcagtcac
      961 gacgacagtg ctgtggtgct gcaattgcag gtgtggacca agagcgaaaa ctactggcag
     1021 ctgcactacc ggctgctgga ggacgtgaaa aaggcgttcg acagccacgg catctctatt
     1081 ccgtatccgc aaatggacgt acatatgccg gaagaaacca cggattctaa ttcctaagga
     1141 aacactgaat aaagc
//
